LOCUS       DBNGZC010000001         2006 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_97936, whole genome shotgun sequence.
ACCESSION   DBNGZC010000001 DBNGZC010000000
VERSION     DBNGZC010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_97936"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="AC16G0_00005"
     CDS             <1..645
                     /locus_tag="AC16G0_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013844.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HIR5098031.1"
                     /translation="AAVAELKRGGSIRAAREGIRQVAELPDAGLADLHAPPVGEAEQP
                     GTLVRVTPGQRVWVRHLGQAGTVLSEASIQGLVEVQLPMGKTRVRVASLSSAPAAASR
                     PTGGVTWRAGAGDGLRDEVNVIGCTVEEATDRVQRFLGDAVLGGLSRVRIIHGKGTGR
                     LRRGMAELLKVHPLVAGFHLASFDEGGAGATIVDLGSRDGEGTPSPLPSGPSHA"
     gene            653..>2006
                     /gene="dnaG"
                     /locus_tag="AC16G0_00010"
     CDS             653..>2006
                     /gene="dnaG"
                     /locus_tag="AC16G0_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR5098032.1"
                     /translation="MIPEDVVAEILQRTDIVELIGAYLPLKAAGRTHKALCPFHTEKT
                     PSFVVNPERQIFHCFGCGEGGDAITFLVKHERLTFPEAARFLAERVGVTIPASRGGGG
                     SPAGDGRLALLEIQRQALEHYRENLRGSEATSVREYLAGRGLTPALIERFQLGYALPR
                     WDGLLRALKQRGHPDKALEAAGLVLPRQGGGGYYDRFRNRLMIPIWDVSGKVVGFGGR
                     ALDPKEVKYLNSPETATYRKGTHLYGLNLAARSIREQKQAIVVEGYFDAIMLHAHGFE
                     QAVATLGTALTTDQARLLGRYASTVVLLFDPDAAGIGAARRNLEHLINVDLDWRIVLL
                     PEGLDPDAFLRAQGAPAFAAALHGAQDLVEFFLDRRVSGLDMADPVQQARAVDALVEV
                     LSTIDNPIRREGYVHQVAQRSALTDRTLLEAVARQRGRAGRQDAAPAAPKPPAAPPTA
                     E"
BASE COUNT          309 a          642 c          729 g          326 t
ORIGIN      
        1 gcggctgtgg cggagctgaa gcggggcggc tccatccggg cggcgcgcga gggaatccgc
       61 caggttgccg aacttccgga tgccggtctg gccgacctcc acgccccgcc cgtgggtgaa
      121 gccgaacagc ccgggaccct ggtccgcgtg acgcctggac aacgggtttg ggtgcggcac
      181 ctgggccaag cgggtaccgt gctctcggag gccagtatcc aggggttggt cgaggtccag
      241 ctccccatgg ggaagacgcg ggtccgcgtg gcgtccttga gttccgctcc ggctgcagcc
      301 tcccgaccca ccggcggtgt cacctggagg gccggggcag gggacgggct ccgggacgag
      361 gtgaacgtga tcggttgcac cgtggaagag gccacggatc gcgtgcagcg gttcctgggg
      421 gatgctgtgc tcggcgggct atcgcgggta cgcatcatcc atggaaaagg gacgggtcgt
      481 ctgcgacggg gaatggccga acttttgaag gttcatccct tggtggccgg gttccacctc
      541 gcctcgtttg acgagggggg cgcgggagcc acgatcgtgg acctggggtc gcgcgacggg
      601 gaagggactc ccagtccgct ccccagcgga ccctcgcacg catgaggaca gtttgatccc
      661 cgaggatgtc gtcgccgaga ttctgcagcg gacggatatc gtcgaattga tcggtgcgta
      721 cctgccgctc aaggcggcgg gacggaccca caaagccctc tgcccgttcc acaccgagaa
      781 gaccccatcc ttcgtcgtca atccggagcg ccagattttt cactgcttcg ggtgcgggga
      841 ggggggggac gccatcacgt tcctggtcaa gcacgaacgc ctgacctttc ccgaggcggc
      901 gcgattcctg gcagagcgcg tgggcgtcac gatccccgct tcccggggcg ggggcggatc
      961 gcccgcggga gacggccgcc ttgctctcct ggagattcag cgacaggcgc tggagcacta
     1021 ccgcgagaat ctgcgcggct ccgaggcgac cagcgtccgg gaatacctgg ccggccgggg
     1081 cctgacgccg gccctgatcg agcggttcca gttgggctat gccctgcccc ggtgggacgg
     1141 gctgctgcgg gccctgaagc agcgcgggca cccggacaag gcgctggagg cggcggggct
     1201 ggtcctcccg cggcaaggcg ggggcgggta ttacgaccga ttccggaatc gactcatgat
     1261 cccgatctgg gacgtgagcg gcaaggtggt cggattcggg ggacgggcgc tggatcccaa
     1321 agaggtgaag tacctgaact cgcccgagac cgccacctac cggaagggga cgcacctgta
     1381 tgggctgaac ctggccgccc gatcgatccg cgagcagaag caggccatcg tggtcgaagg
     1441 ctacttcgac gcgatcatgc ttcacgccca tgggttcgag caggctgtgg ccaccctggg
     1501 cacggccttg accacggatc aggcgcgcct gttgggccgc tacgcctcga ccgtggtcct
     1561 gctcttcgat ccggacgcgg ccgggatcgg cgcggcccga cggaacctgg aacatttgat
     1621 caatgtggat ctggactggc gcattgtgtt gctccccgaa ggactcgacc cggacgcctt
     1681 tctccgggct cagggcgccc cagcctttgc cgcggccctc cacggggcgc aggatctggt
     1741 ggaatttttc ctggatcgtc gggtctccgg cctggacatg gctgacccgg tccagcaggc
     1801 tcgcgcggtt gatgccctgg tggaggtcct gagcaccatc gacaatccga tccgccggga
     1861 agggtacgtc catcaggttg cacaacgcag tgcgctcacg gaccgcacgc tgttggaagc
     1921 ggtggcgagg cagcgcggac gtgcggggcg tcaggatgcc gcccctgcgg ctccgaagcc
     1981 gcctgcggct ccccccacgg ccgagg
//
LOCUS       DBNGZC010000002         1981 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1859082, whole genome shotgun sequence.
ACCESSION   DBNGZC010000002 DBNGZC010000000
VERSION     DBNGZC010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1859082"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1614)
                     /locus_tag="AC16G0_00015"
     CDS             complement(<1..1614)
                     /locus_tag="AC16G0_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5098033.1"
                     /translation="MFRRILVAYDGSAAARAALRVGIQLAQAPETELASVSVEESLPR
                     YAATMGEVEEAKEDIEQHGRQLTAEAQELARAGGVTVETEIRQGHEVEMILALARERR
                     ADLLLIGYQGHSRIFKGLLGSTALTLARVAACSVLVVRPLEFVPGSGGGFQRVLVGLD
                     GSPNGRLALHAAIDLAKPSGGTVIGLTVLETSPLGVSGGLDSGYAQRVQTAAEEHGRT
                     AGAQFEGVTRVGHAVQALCDQAEEGRADLIVLGATGLARPWSPTIGGTATGVVSQAPC
                     SVLLVRPPQAALRVRDVMVRAVSSVSEDESLVRVVELLLRRNIKALPVVDYRRHVVGI
                     ITGGDLLTRGDAGVRLSIQRELPPAIWRGNLCELVRGAKTARDVMTPGVYTVKAEADL
                     LTAIRLMARHRVKRLPVVDEEGELIGILNRADVLRVLAAQPEPAMPAELEAQAQAGTV
                     GDALTTGIPVVSSNTSAEDVLVKLLECPLRRVVVAEPDGRVLGLISDRDLLLRAGPDL
                     RPWVLRALTGLRPVRRPGEAGKELAGPEGP"
BASE COUNT          318 a          750 c          607 g          306 t
ORIGIN      
        1 cggcccctcc gggcccgcca actccttgcc ggcctcaccc gggcggcgta caggccgtag
       61 cccagtgagc gcgcggagga cccacggcct gaggtcggga ccggcacgga ggagcaggtc
      121 ccggtcgctg atgagcccga gcacccggcc gtccggctct gccactacca cccgccgcaa
      181 tgggcactcc agcagcttca ccagcacgtc ctcagccgag gtgttcgaag acacgaccgg
      241 tatcccggtg gtcagggcgt cacccaccgt gcctgcctgc gcctgagctt ccaactccgc
      301 tggcatggcc ggctccggct gggccgcgag cacccggagc acgtccgcgc ggttcaggat
      361 cccgatcagc tccccctcct cgtcaaccac gggcagccgt ttcacccggt gccgcgccat
      421 gagccggatg gcagtcagga gatccgcctc ggcctttacc gtgtacacgc ccggagtcat
      481 cacgtcgcgg gccgttttcg caccccggac aagctcgcaa aggttcccgc gccaaatcgc
      541 cgggggcagc tcccgctgga tgctcagtcg taccccggca tctccccgag tgagcagatc
      601 ccccccggtg atgatcccca ccacgtgccg gcggtaatcg accaccggga gcgccttgat
      661 gttgcgccgc aggagcagct ccacgacccg aaccagcgac tcgtcctcgc tcaccgagga
      721 cacggcacgc accattacgt cccggaccct gagcgctgcc tgcggcggcc ggacgaggag
      781 gacggagcag ggggcctggc tcacaacacc ggttgccgtc cccccgatgg tgggactcca
      841 cggacgcgcc agcccggtcg cccccaggac gatcaggtcg gcccggccct cctccgcttg
      901 gtcgcagagc gcctgtaccg cgtggccgac tcgcgtgacg ccctcgaact gcgcgcctgc
      961 cgtgcgcccg tgttcctcag ctgccgtctg gacccgctgg gcgtatccgg aatcgagccc
     1021 cccagacaca cccaaggggg acgtttccag gacggtcagc ccgatgaccg tcccgccaga
     1081 gggcttcgcc agatcgatcg ccgcgtgaag agccagccgc ccgttgggcg acccgtccag
     1141 accgacgagg acacgctgga agccacctcc gctccccggc acgaattcga ggggccgcac
     1201 caccaagacc gagcacgccg ccacccgggc gagcgtcaag gctgtgctgc ccaggaggcc
     1261 tttgaagatg cggctgtggc cttgatagcc gatcaggagg aggtccgccc gccgctcgcg
     1321 ggcgagggcc aggatcatct ccacctcatg tccctgtcgg atctccgtct ccaccgtgac
     1381 accgcccgcc cgtgccagtt cctgcgcttc ggccgtcagc tgacggccgt gctgttcgat
     1441 gtcttccttc gcttcctcca cctcgcccat cgtcgcggcg tagcggggga gcgattcctc
     1501 caccgagacg ctggccagct ccgtctccgg agcctgggcc aactggatcc cgacgcggag
     1561 cgccgcccgc gcagccgcag acccgtcgta ggccaccagg attcgccgga acatggcgcg
     1621 cctcccttgt cagaagagcc gtagcagcag caccacgagc acgatggcac agatgcacac
     1681 gacatgctgg ttcacgcttc ccgactgcat gtcttgggcg cgccgcatca gcactatggt
     1741 cggcgcggtc ggggcaggcc ctgcgcctcc atgtagcgta gaagccgaac gatctgctcc
     1801 tgtgtccgac tggtcgtctc ctatagcgat ggcttcgcga aactctcttc cgggaaagcg
     1861 accgtgagct ggtatccgcg cctcgccaac cggaccggga tcacaacttc ctgttgttgc
     1921 ggatcattgt gaggttcgag gacttgcacc tcaagctccg gatcctcaac cactgcccaa
     1981 g
//
LOCUS       DBNGZC010000003         3857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1272232, whole genome shotgun sequence.
ACCESSION   DBNGZC010000003 DBNGZC010000000
VERSION     DBNGZC010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1272232"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="AC16G0_00020"
     CDS             <1..218
                     /locus_tag="AC16G0_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5098034.1"
                     /translation="FLKAIRQQGTDPAFKVKSGTSDMNVVGPAWNCPILAYGPGDSSQ
                     DHTPHEHLDLAEYHRAISVLTRVLRLL"
     gene            657..1874
                     /locus_tag="AC16G0_00025"
     CDS             657..1874
                     /locus_tag="AC16G0_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIR5098035.1"
                     /translation="MQETRRIGLLGWILIGFVVGVLFGILFKGPSLTISWVGDLFLRL
                     LKMLIVPLVFFSLLTGTASISDPIKLGRVGVKIIGIYLATTAIAVIIGLLLATFFNPG
                     VGMNLAPVAGFKAPAIPALKDVMLNLVPTNIFGSLTSGDILPIIVFSIFFGIAAVLTG
                     EKGKPVVRVADAAAETMYKLTAIVMWFAPLGVFSLIATTVAKQGPAVLLPFLKFIIVC
                     YIAYFIHVAVIYSSIVKAMAKVNPLRFFKAISEVMLMAFVTRSSNATLPVTLRVSEEN
                     LGLPRSITSFTLPLGATVNMDGTAIYMGISALFVAAVYSIPLGFSHYLMIILLATLGS
                     IGAAGVPGAGLIMLAMVLQGVGLPLEGIGLIAGIDVILDMGRTCLNVTGDVACTAAVA
                     YSEGEFSPKMVNA"
     gene            2383..>3857
                     /locus_tag="AC16G0_00030"
     CDS             2383..>3857
                     /locus_tag="AC16G0_00030"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit 5 family
                     protein"
                     /protein_id="HIR5098036.1"
                     /translation="MGMSQNELLLPLIVFVPFAAAILALTVGRITGRHTGILMVLAAL
                     ASFAMALRLLQAGWAGSPVIFTRPWIPALNVNLGLRGDSFGLFFALLISGIGILVGVY
                     SLGYMPKLDPGRLGQFYAALLAFMGSMLGVALADDLVVLFILWEITSLTSFILIGFWY
                     EQEASRKGAMTALLITALGGLTMMIGFIIVGITAGTFSLGALTQNADLARRLTGSPLF
                     LPALLLVIIGAMTKSAQWPFHFWLPGAMVAPTPVSTYLHAATMVKAGIFLLGRMLPIF
                     GGTPVWAGILVPIGLATFLLGAIQAFQDTDLKGILARTTVSTLGLVTMLYGLGAAGQD
                     ALQMFSHGTYKGALFLVAGIIEHATHTRDLRQLGGLRRRMPVTFGICLLGALSLAGVF
                     PTFGFLAKEALYTDLLSGTMSGGWVPSWLILVLGIVANAFLFAVGCRIVIGIFLGSPT
                     EHTQHAHEAGPALWLPAGALAGGALLLGVIAPVTGHLVSRFS"
BASE COUNT          615 a         1273 c         1159 g          810 t
ORIGIN      
        1 ccttcctcaa ggccattcgc cagcagggga ccgatcctgc cttcaaggtc aagagcggga
       61 catcggacat gaacgtggtc ggccccgcct ggaactgccc catcctcgcc tacggcccgg
      121 gcgattcgag ccaggaccac accccccacg agcatctcga cctcgcggag taccacaggg
      181 ccatctcggt cctcacccgg gtcctcaggc tgctctgaat cgcagaaacg attcatcagg
      241 cctacggccc tcacagcctc caagcatata agcgcgggat ttcgagggtg ctgccctgtc
      301 actattcact gcacaaccga accaagcttg ctctttaacc ttgacatcgg atgaagagga
      361 ggaatagctt ggcctcgcat tggacgggtc cctgctggat gatggagcaa cggtgacggg
      421 tcccgcgtgt actctcggac gaagaatcag cacgccacgc atcctacgtg aatagccttt
      481 cgggaaggct actcccacgg ggcacttttt catcggaccc ctgttcctca cggggaatca
      541 gccgaaggag gtgaagcgac aactcgaatt gcctcgggaa attgacatga ccatgccatt
      601 cgattggatg gagaccggga ggggggacgg tcaccttgtc aaggaaggag atggtcatgc
      661 aggagacaag acgcatcggt ttgctcggat ggatcctgat cggatttgtc gtaggtgtct
      721 tgttcgggat cctgttcaag ggacccagcc tcacgattag ctgggtgggc gacctgttcc
      781 tccgcctgct gaagatgctc atcgttcccc tcgttttctt ctcgctgctc accggcacgg
      841 cctcgatcag cgaccccatc aagctcgggc gtgtgggcgt gaagatcatc gggatctacc
      901 tggccaccac cgccatcgcc gtcatcatcg gcctgctctt agcgacgttt ttcaaccccg
      961 gcgtcggaat gaatctcgcc ccggtggcgg ggttcaaggc gccggccatt cccgctttga
     1021 aggacgtgat gctgaacctc gttcccacca atatctttgg ctcactcact tcgggagaca
     1081 tcctgccgat catcgtcttc tccattttct tcgggatcgc cgcggtcctg acgggggaaa
     1141 aggggaagcc cgttgtccgc gtggctgacg ccgcggccga gaccatgtac aagctcacgg
     1201 ccatcgtgat gtggttcgcg ccccttggcg tcttctcgct cattgctaca accgtcgcca
     1261 agcagggacc agcggttctc ctgccgttcc tcaagttcat catcgtctgt tacatcgcct
     1321 acttcattca cgtggcggtg atctattcct cgatcgtcaa ggctatggcc aaggtcaacc
     1381 ccctgcgctt cttcaaagcc atatccgagg tcatgctgat ggccttcgtc acgcgcagca
     1441 gcaacgccac gctcccggtg acgctgcggg tctccgagga gaatctgggc ctaccgcgga
     1501 gcatcaccag cttcaccctc cccctcgggg ccacggtcaa catggacggc acggcgatct
     1561 acatggggat ctctgcgctg ttcgtcgctg ctgtttactc catcccgctg gggtttagcc
     1621 actacctcat gatcatcctg ctggccaccc tgggctcgat cggcgcggcg ggggttcccg
     1681 gcgcaggcct gatcatgctg gccatggtgc tgcaaggggt cggattaccc ctggaaggga
     1741 tagggctcat tgcggggatc gacgtgatcc tggacatggg ccggacgtgc ctcaacgtga
     1801 cgggcgacgt ggcctgcacc gcggccgtgg catactcgga aggagaattc tctcccaaga
     1861 tggtaaacgc gtagttctcg tgctcgacga cgccaactat tggggcatcg tccccggatg
     1921 ctaccgcatg ccgggggcga tgccctggca tcgtgctgtg gcccatcggt ctcacaggac
     1981 gagtatccgg actgtttgat cctgcgggac caggattacc ctccacgcct gggtttgcca
     2041 ccccgccgcc gctcgacccg gccggcgctt gtgtggttgc tcagccaatc cgtttgggct
     2101 ctgttcaaat cgtttgaccc agggagcgat ttctggcata tacacacctc ggcaaagctg
     2161 cagcgcgatc cggtgtgtat gccgcggcca tggctccgtc tccaaaacgg agcaccaacc
     2221 atcggagggg gagaggacgg agaccgatgg gatacggcaa tctggaggac gtcatccccc
     2281 ttcccgctgt cgtgcctcgc gccgtagacg ccttccggtg ggtcccataa cgcacccccc
     2341 atccctggcg ctttccttca cgcggtcatc gtaccgaggc agatgggtat gtcacaaaac
     2401 gagttgctgc tcccgttgat cgtgttcgtt cccttcgccg cggcgatcct ggctctgacc
     2461 gtcgggcgga tcacggggcg acacaccggg atcctgatgg tcctggcggc cctggcctcc
     2521 ttcgccatgg cgctccgact cctccaggcg ggctgggccg ggtcccccgt catcttcacc
     2581 cggccctgga tcccggccct caacgtcaat ctgggattgc ggggcgacag ttttggactc
     2641 ttcttcgccc tcctcatctc cggcatcggc atcctggtgg gtgtctactc cctcggctac
     2701 atgcccaagc tggatcccgg ccgtctgggc cagttctacg cggcgctgct ggccttcatg
     2761 ggctcgatgc tgggggttgc cctggccgac gacctcgtgg tcctcttcat cttgtgggag
     2821 atcaccagcc tcacgtcctt catcctcatc ggcttctggt acgagcagga ggcgtcccga
     2881 aagggcgcca tgacggccct gctgatcacg gccctgggcg gcctcaccat gatgatcggc
     2941 ttcatcatcg tcgggatcac cgcggggacc ttcagcctcg gcgcgctgac gcagaacgcc
     3001 gacctcgccc gccgtctcac cgggtctccg ctcttcctgc cggccctgct gctggtgatc
     3061 atcggcgcca tgaccaagtc ggcgcagtgg cctttccact tctggttgcc cggcgccatg
     3121 gtggccccga caccggtctc gacctatctg catgccgcca ccatggtcaa ggcgggcatc
     3181 ttcctgcttg ggcggatgct ccccatcttc ggagggaccc cggtctgggc ggggatcctg
     3241 gtccccatcg ggctcgccac cttcctgctg ggggccatcc aggccttcca ggacaccgat
     3301 ctcaagggaa tactggctcg cacgacggtc tccaccctgg gcttggtcac catgctctac
     3361 ggtctcggag cggcgggtca ggatgctctc cagatgttct cccacgggac gtacaagggg
     3421 gcgctgttcc tggtggccgg catcatcgag cacgccaccc acacgcgcga cctgcggcaa
     3481 ctcggcggcc tgcggcggcg catgccggtg accttcggga tctgcttgct gggggccctt
     3541 tcgttggcgg gcgtattccc cacgttcggc tttctggcca aggaggccct gtacaccgac
     3601 ctcctgagcg ggaccatgtc gggcggatgg gtgccatcgt ggttgatcct ggtcctggga
     3661 atcgtggcca atgcgttcct cttcgccgtg ggctgccgga tcgtgatcgg gatcttcctg
     3721 ggtagtccga cggagcatac ccagcacgcc cacgaggcgg gcccggccct ctggttgccg
     3781 gcgggagcgc tggcgggggg cgcgctgctc ttgggagtga tcgcgccggt cacgggtcat
     3841 ctggtgagtc gcttctc
//
LOCUS       DBNGZC010000004         2322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1663642, whole genome shotgun sequence.
ACCESSION   DBNGZC010000004 DBNGZC010000000
VERSION     DBNGZC010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1663642"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="AC16G0_00035"
     CDS             <1..420
                     /locus_tag="AC16G0_00035"
                     /inference="COORDINATES: protein motif:HMM:NF025019.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HIR5098037.1"
                     /translation="APKVYGFDTGFVCYHRGWSALRADDLGLLWEHFVLNELLAALQG
                     EPIHYWRDKAGHEVDFVLKRRGQPPVAIECKWSAAEFSTENLRAFRYRYPDRENVVVA
                     QDADRPLIRTLGAIRVRVEPLSGLIQRLQGKPGKPAR"
     gene            723..2322
                     /locus_tag="AC16G0_00040"
                     /pseudo
     CDS             723..2322
                     /locus_tag="AC16G0_00040"
                     /inference="COORDINATES: protein motif:HMM:NF013761.7"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
BASE COUNT          430 a          743 c          760 g          389 t
ORIGIN      
        1 gcgccgaagg tctacggctt cgataccggc ttcgtctgct accatcgggg ctggtcggcg
       61 ctccgggccg acgacctcgg gcttctctgg gagcacttcg tgctgaacga actgctcgcc
      121 gccctccagg gcgagcccat ccactactgg cgcgacaagg cggggcacga ggtggacttc
      181 gtcctgaagc gccgaggcca gcccccggtc gcgatcgagt gcaagtggtc ggccgcggag
      241 ttttcgaccg agaacctgcg ggccttccgc taccggtacc cggaccgcga gaacgtggtg
      301 gttgcccagg acgccgaccg tcccttgatc cgcacgctgg gagcgatccg cgtgagggtc
      361 gagccgcttt cgggtctgat ccagcgcctg caaggcaagc cggggaagcc tgcacgatag
      421 aggagccgcc ggctgccaat ccccacggac tcgtgctccg gtcgagggtt gccaccggcc
      481 cacaagccaa ggcatacgcc taggcgccaa cccgacgatt tgctctgccc aaccggcggt
      541 ccgccctccc ggcatcacag ttacctccgc acctatgtcg ctgctgaatc tccgctgaag
      601 cgtgcctttc aacagtatgc atggacgcca ggctgggtct ccagtagaat catctcggcg
      661 gctagctcgt ggaggattgg tggcgcgatg aatcctgcgc gaggaggaat gtcatgggca
      721 cgatgcgtgg ggatgcggtg gtcgagaagg gcttggagat cttccggctc atggaggcgg
      781 agcccccggc ggtcttcaac aagaagttct gggtggggaa gatgatggag gcggcgatgg
      841 accacccgga actgaagatc gccctgttcc ggttcatcga cgtcctgccc accctgacga
      901 cgacacagca gctctcccgg catatcaagg agtacttcct ctccgagggt tcccaggttt
      961 ccggcgtgat gaagaccctg ctgtccggcg ccaccaccgg cctcaccgcg ggaatcgccg
     1021 cctcggtgat caagcagaac atcaccagtt tctccaagaa tttcatcgtt ggggagaatc
     1081 cccaggacgc catccggacc ctcaggcaga tctggaacga gggcaagagc ttcaccctgg
     1141 atctcctcgg ggaggcggcc gtctcggagg tcgaggctga caggtatcgc gacctgtacc
     1201 tctcggtggt ggacattctg tcgaaggaga tggcctcctg gccggcccag gacccggagc
     1261 atgagaagca tttcccccgg ctcaacctct cggtgaagat cagctccctc tactctcgca
     1321 tcggacccat gaactacgag gacagcctgt agcaggtgaa aaaccggctg cgaccgatct
     1381 tccataaagt caaagcggcc ggcggcgccg ccctcatgga catggaggtg cggggcctca
     1441 aggacctgac gctcgatgtg ttcttcggcc ttctggagga gccggagttc cgggggtggg
     1501 agcacgcggg gatcgccctc cagacatacc tcacgtccgc gcagcaggac gtggcgcgcg
     1561 tcatcgactg ggcgaagacc cacgaccacc ggatcggtat ccggctggtc aagggcgcct
     1621 actgggagta cgagaagacg atcgcaggcc agcgggggtg gccggtaccg gtcttcaccg
     1681 tcaaggccca caccgactgg agctacgagc ggtgtctgga tctgatcctg gagaatcacc
     1741 agcatatcac cacggccgtg gcctcccaca atgtgcgctc cattgccaag gcgctggtca
     1801 cgatccagca actgaaggtg ccgaagaacc ggtacgagtt tcaggccctg tacggaatgg
     1861 ccgagccgat caagcacgcg ctgaagcaga tgggccacgt cgtgcgggag tacgctccgg
     1921 tcggcgatct gattcccggc atggcctacc tggtgcgccg gctgctggag aacacctcca
     1981 acgaggggtt tctgcggaag aagttcgtcg gaaacgtcga tcccgaggcg ctcctggccg
     2041 agccggaccc gtacccgggg gagcctgtgc cagcgaaggc cgggggactc acgcccttcg
     2101 cgaacgagcc ccctctcgac ttcgcggttg ggaagatccg cacggccttc cgggaggcca
     2161 tcgccagcgt ccgcctggag atggggcggg agttcccggt gatctacgct gggaaggagc
     2221 atcggacccg ggatcggctt gagtcgcgga accccgccag cccgacggaa gtggtctgca
     2281 ccacctcgac cctcagccgg gaaatggccg acgaggcgct gg
//
LOCUS       DBNGZC010000005         3523 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2642083, whole genome shotgun sequence.
ACCESSION   DBNGZC010000005 DBNGZC010000000
VERSION     DBNGZC010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2642083"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            507..968
                     /locus_tag="AC16G0_00045"
     CDS             507..968
                     /locus_tag="AC16G0_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008920364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098038.1"
                     /translation="MKQIMWAIGLAILVLACAMVPAEAASGGGFHGGGGSFHGGGGGF
                     HGGGGNFHGGHFHGGTRVFIGGGFGWWGWPGYWGWPGYWGPPYPYYGYYGASPVVVQQ
                     GPTDYIQQDPSPSSAPQYWYYCQEAGAYYPYVKECPGRWMQVVPQPASPGR"
     gene            complement(1333..2193)
                     /locus_tag="AC16G0_00050"
     CDS             complement(1333..2193)
                     /locus_tag="AC16G0_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009178608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098039.1"
                     /translation="MIPSIPQPAYARRLYTAYVLAFFVYLFAPLVVVAVLAFNDSLFP
                     ALPWRGFTLDWFLAQGPRRVGLLYDQGLLRGMATSAKVAVWVAGLSVVVGTCNAFLFE
                     QEEFPCKQVLYALSLTPLVIPGVILGISILLFSSTLATAIEGWTGVEMDVLRPGLPLV
                     VLGQFSFITTLTTLVISARLRKFDRSLEEAALNLGASRAVAIGTVTLRFLRSALIGAG
                     AVAFLMSFENFNTTLFLVGSDATLPITMFINLRDGSTPVINAVSLVLMVGSATLGLIG
                     IFGGFDAKKS"
     gene            complement(2190..3011)
                     /locus_tag="AC16G0_00055"
     CDS             complement(2190..3011)
                     /locus_tag="AC16G0_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098040.1"
                     /translation="MTPAALWLLLLIVLPHVDMFLLSIRSKGAAGALGLSNYARFFAE
                     PIYWNTFARTAIYSVLATLLTLIIAFPVAYYIAKVAPGRGKTILFLLCLLPFWVSELV
                     RTYGWMILLRESGVLSGFLVWAGLVAKPVEMLYNDVAIMIGLIYTSMLFMVVPLVTVL
                     ESLDDSLIEAAYDLGGTAPSILWEIVIPHAMPGIAAGSIVVFMLTLGNYLTPTLLGGK
                     SSLWFTEQIYNQFIVRFNWEQGAAFGFLLLILSSAIVWIGLKLTGQTLAGSIQSR"
     gene            complement(3050..>3523)
                     /locus_tag="AC16G0_00060"
     CDS             complement(3050..>3523)
                     /locus_tag="AC16G0_00060"
                     /inference="COORDINATES: protein motif:HMM:NF019998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HIR5098041.1"
                     /translation="MNQGRFEQVDTPQGLYYHPRTAFVAGFVGESNRWQGQVIAVSGQ
                     EARVRTSDGLELVARTSERLTASQTVDLFIRPEAISLAREAGEIGTLANRIAGSVESI
                     LFDGANSRILVKELQTKGEVTATLPQTGRFADLAPGQTVHLGWDAGGGLCFERGR"
BASE COUNT          692 a         1068 c         1138 g          625 t
ORIGIN      
        1 gcgatcatgg gaggacctca ccctacgaat ccctgtgcgc atgtcggaga gcccggcggc
       61 gcgccactcg accgctgagc gcacgtcgct tcgacctagc ctctcaggat gggattcctc
      121 ctatggcggt cacacgacta cccagcgtgc tgttgccacg gtaggatgtt gtggccgccg
      181 ccatctagta cgagaaggcg gtatggctat ttgtagatgt tcttgacagg gcgagaattc
      241 cgggagtacc ttcttagcgt tgccccccac attgatgatc aatgggagtg gcagcatggg
      301 gtcggccagg gacggccctc aacccccgag acgggcaaac ggttctgtgc accccgcttg
      361 agggggcgtt tgggttgcta tccggtctat gtcggtgtgc cctggggtga ctggaccgga
      421 tgtcatggat gttgccacaa gtggtgcggc tgttatagca ggatgagcct gatgtagtgt
      481 tcagggatgg cagtgggaga ttggccatga agcagatcat gtgggcaatc ggattggcaa
      541 tcttggtgtt ggcttgcgcc atggtccctg cagaggcggc gagcggcggg gggttccatg
      601 ggggaggcgg tagcttccac ggaggaggcg gtggattcca cggtggaggc ggtaacttcc
      661 acgggggcca cttccacgga ggcactaggg tctttatcgg gggtggtttt gggtggtggg
      721 gatggccggg atattgggga tggccgggat attgggggcc cccctaccct tactatggct
      781 actatggtgc ttctcctgtg gtggtccagc aggggcctac ggattacatc cagcaggacc
      841 cctcgccttc atccgccccg cagtactggt actactgcca ggaagcgggg gcgtattacc
      901 cgtacgtcaa ggaatgccca ggcaggtgga tgcaggtcgt tccccagcca gcctcacctg
      961 gccgctagat atcagaatcg ccgaggccag ccaatccccc gggtaggtcc ttagacttac
     1021 cgggggattc ttttttcgat tgggtgaccg ttggatggat cttgaggact cccgtgccgg
     1081 ttggtagctc tgctactccc ccgcgacccc tattgagcct ccagggcctc tgcccgccgg
     1141 tcgcagcgcc actcacaggt gatactcggg ttacggcctc gatttcgtaa tctcaattca
     1201 gaccaggttt cggtttgaac cctcgtggtc cgcgagaatc atgtcggcac gctcatcgtg
     1261 taccgctccc gctttcctgg acacgacata agcatcatca cgcacgccgc gtcggccgcg
     1321 gatttctcag gcttaagatt tcttggcatc gaaaccgccg aagatgccga taaggccaag
     1381 ggtcgccgag ccaaccatga gcacgaggct caccgcgttg atcaccgggg tggagccgtc
     1441 ccgcaggttg atgaacatcg tgatgggcag cgtggcgtcc gagccgacca ggaagagcgt
     1501 ggtgttgaaa ttctcgaacg acatcagaaa tgccaccgcc cccgcgccga tgagggccga
     1561 cctgaggaac cggagggtca ccgtaccgat ggcgactgcc cgggaggcac caaggttgag
     1621 cgctgcctct tccaggctgc ggtcaaactt acgcagacgg gcggagatga ccagggtggt
     1681 tagcgtggtg atgaaggaga actgccccag gacaaccagc gggagacccg gccgcagcac
     1741 gtccatctcc accccggtcc acccctcgat ggccgtagcc agggtgctgg aaaacaacag
     1801 gatcgagatc cccaggatga cccccgggat gaccagtggc gtcaacgaca gagcgtagag
     1861 gacctgcttg cacgggaact cctcttgctc gaacagaaag gcgttgcagg tcccgaccac
     1921 caccgacagc ccggccaccc agaccgcgac cttcgcgctg gtagccatcc cacgaaggag
     1981 tccctgatca tacagcaagc cgacccggcg ggggccctga gccaagaacc agtccagggt
     2041 gaatccccgc cagggcaagg ctggaaacag gctgtcgttg aacgcgagca cggccaccac
     2101 caccagcggc gcgaacagat agacgaagaa ggcgagcacg taggccgtgt agagacggcg
     2161 cgcataggcc ggctggggga tggaggggat catcgcgact ggatggatcc cgccagcgtc
     2221 tggcccgtga gcttcagccc gatccagacg atggccgatg acaggatgag cagcaggaag
     2281 ccgaaggccg ccccctgttc ccagttgaac cgcacgatga actggttgta gatctgctcc
     2341 gtgaaccaga ggctggattt tccgccgagc agcgtcggcg tcaggtagtt ccccagggtc
     2401 agcatgaaga ccacgatgct gccggcggcg attcccggca tggcatgcgg gatcacgatc
     2461 tcccacagga tggagggggc ggttccgccc agatcgtatg cggcctcgat caggctgtcg
     2521 tccagactct ccagcaccgt gaccagcgga accaccatga acagcatgga ggtgtagatc
     2581 agaccgatca tgatggcgac gtcgttgtag agcatctcca ccggcttggc caccaagcca
     2641 gcccacacca ggaagccgct caggacaccg ctctcgcgca gcaggatcat ccagccatac
     2701 gtccggacca gctcgctcac ccagaatggg agcaggcaca ggaggaagag gatggtcttt
     2761 ccgcggcccg gtgccacctt ggcaatgtaa taggccactg gaaacgcgat gatcagcgtg
     2821 agcagggtcg cgaggacgga gtagatggcg gtcctggcga aggtgttcca gtagatcggc
     2881 tccgcaaaga acctcgcata gttcgacagc ccgagtgctc ccgcggcccc cttcgaccga
     2941 atggagagga ggaacatatc cacgtggggc aacacgatga gcaggagcag ccacagcgcc
     3001 gccggggtca tgaagaccgc caagccgacg tagccgcgga cccgcgggct caccgtcccc
     3061 tctcgaagca aaggccaccc cctgcgtccc agcccagatg gacggtttgt ccgggtgcca
     3121 gatcggcgaa gcggcccgtc tgcggtaagg tggcggtgac ctcccccttg gtctggagtt
     3181 ccttcaccag gatccggctg ttggccccgt cgaagaggat gctttcgacg gacccggcaa
     3241 tgcggttcgc caaggtgccg atttcccccg cttcccgtgc caggctgatc gcctccggcc
     3301 ggataaagag atcaacagtc tggctggcgg tcaagcgttc ggaggttcgg gcaaccagct
     3361 ccagcccatc gctcgtgcgc acccgtgcct cctggccgga gacggcaatg acctgtccct
     3421 gccagcggtt gctctccccc acgaacccgg cgacgaaggc ggtgcgcggg tggtagtaga
     3481 gcccctgagg agtgtccacc tgctcgaagc ggccctgatt cat
//
LOCUS       DBNGZC010000006         2325 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_196471, whole genome shotgun sequence.
ACCESSION   DBNGZC010000006 DBNGZC010000000
VERSION     DBNGZC010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_196471"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..944)
                     /locus_tag="AC16G0_00065"
     CDS             complement(54..944)
                     /locus_tag="AC16G0_00065"
                     /inference="COORDINATES: protein motif:HMM:NF014046.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="damage-control phosphatase ARMT1 family protein"
                     /protein_id="HIR5098042.1"
                     /translation="MRTTPTCLTCVLGDVYAAARQVTPDAQTQLKVAKDAMEFLAGSF
                     GHEKVPSYYITEVHRILKRDTGVATPFAESRRQLNQVAMEIAVRLQAEAEKLEGLARF
                     RFLALWALAGNSLDSRTVGIGYDFNPDKTLHHMQVYVDRGIAVDHLDRLYQLVLARPP
                     ILYIHDNVGEIALDKLFIQELRRHGCRVTSALRGGPITSDATMEDGRTVGLDKAVDRL
                     IQAGPDTLGISWEEASPELSQAMQEASLIIAKGQANYYVFSEHRAKLPGQVFCLFTIK
                     CQPVADVIGVTPKQAVGIFL"
     gene            complement(941..1759)
                     /gene="thiD"
                     /locus_tag="AC16G0_00070"
     CDS             complement(941..1759)
                     /gene="thiD"
                     /locus_tag="AC16G0_00070"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIR5098043.1"
                     /translation="MSGRSFPRALTIAGSDSGGGAGIQADLKTFTAFKVFGMSAITGL
                     TAQNTRGVQGVELPTCNFIRAQLRAVLEDIGADAAKTGMLATGEIIQTVAEAVREFGL
                     QNLVVDPVMIAASGDSLMQHEAAPVMREHLFPLATVVTPNLDEATFLCERKIRTVEEM
                     EAAARTIHAMGPRYVLIKGGHLPGEQVVDLLYDGKEFVRRESRRIPGEFHGTGCTLSS
                     AIAAGLAKGLGVRDAVATAQGFMELALKTAPAVGRGRRPLNHFPEGFVPPEDRR"
     gene            complement(1774..>2325)
                     /locus_tag="AC16G0_00075"
     CDS             complement(1774..>2325)
                     /locus_tag="AC16G0_00075"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR5098044.1"
                     /translation="WQCLYPLGHFPLVSQQATLQGLDPYLVTALIREESAFAPRAVSR
                     AGARGLMQLMPETAEQVARRYKVRLPSAPPLESPEVNIQLGTMHLAELLRDNGGSLSL
                     AIASYNAGRQQVQRWIQRFGFTTEEEFTEDIPYNETRNYVKRVLGSYERYTSLYGKKK
                     AESREPRAAKTRVRHAAARRSHR"
BASE COUNT          362 a          807 c          735 g          421 t
ORIGIN      
        1 tctttgcacg acccgaacag tcgattcgcc accaagaagg gctgcagagc aactcacaga
       61 aagattccca ccgcctgctt gggcgtgacg ccgatcacgt cggctacggg ctggcacttg
      121 atggtgaaca gacagaagac ctggccgggg agcttggcgc gatgctcgga gaagacgtag
      181 taattggcct gccccttggc gatgatgagc gacgcctcct gcatcgcttg gctgagttcc
      241 ggcgaggcct cctcccagga gatccccagg gtgtccggac cggcctggat gagccggtcc
      301 accgctttat ccaggccgac agtccggccg tcctccatgg tggcgtcgga cgtgatggga
      361 ccgccccgca gggccgaggt gacgcggcag ccgtgtcgcc gcagctcctg gatgaaaagc
      421 ttgtccagag cgatctcgcc gacgttgtcg tggatgtaga ggatcggcgg cctcgccagc
      481 accagctggt acaggcggtc cagatggtcc acggcgatcc cgcggtccac gtagacctgc
      541 atatggtgta gcgtcttgtc ggggttgaag tcatacccga tcccaaccgt ccgggagtcc
      601 aggctgttcc ccgccagcgc ccagagcgcc aggaagcgga agcgggccag tccctccagt
      661 ttctccgcct ccgcctggag acgcacggca atctccatgg ccacctggtt cagttgccgg
      721 cggctctccg caaagggcgt agccacgccc gtgtcccgct tgaggatccg gtgcacctcg
      781 gtgatgtagt aggaaggcac cttctcgtga ccgaagctcc ccgccaggaa ttccatggcg
      841 tccttggcga ccttgagctg tgtctgagcg tcgggcgtga cctgccgtgc cgcggcatac
      901 acgtcgccca ggacgcaggt gaggcaggtg ggcgtggttc tcatcgtcgg tcctccggcg
      961 gcacgaaacc ttcggggaaa tgattcaacg gccgcctccc ccgtccgacc gcgggggccg
     1021 tcttgagcgc cagctccatg aatccctggg ccgttgccac ggcgtcccgc acaccgagcc
     1081 ccttggccag cccggcggcg atggccgagg agagggtgca gccggtcccg tgaaactccc
     1141 ccgggatgcg ccggctctcc cgccggacga actccttccc gtcgtagagg agatccacca
     1201 cctgctctcc cggcagatgt cctcccttga tcaggacgta gcggggtccc atggcgtgga
     1261 tggtccgcgc cgccgcctcc atctcttcca ccgtccggat cttccgctcg cagaggaagg
     1321 tcgcctcatc caggttgggc gtgaccaccg tggccagcgg gaacaggtgc tcgcgcatca
     1381 ccggtgcggc ctcgtgctgc atcagggaat ccccgctggc ggcgatcatc accgggtcca
     1441 ctaccaggtt ctgcagaccg aattcccgga ccgcttccgc caccgtttgg atgatctcgc
     1501 ccgtggccag catccccgtc ttggcggcat cggccccgat gtcttccagc accgcccgga
     1561 gctgcgcccg gatgaagttg caggtgggca gctccacccc ctgaacaccc cgcgtgttct
     1621 gggcggtcag cccggtgatg gccgacatgc cgaacacctt gaacgccgtg aaggtcttga
     1681 ggtcggcctg gatccccgcc ccgccgcccg aatccgatcc ggcgatggtg agcgctctcg
     1741 ggaaactcct gccactcatt ccctgttgcc tccctagcga tgtgaacgcc gcgcggccgc
     1801 gtgtctcacc cgtgtcttcg cggctcgcgg ttctcggctc tcggcttttt tcttcccgta
     1861 caggctcgtg tatcgctcgt agctgcccag gacccgcttc acgtaattgc gggtctcgtt
     1921 gtacggaatg tcctcggtga actcctcctc ggtggtgaac ccgaagcgct ggatccatcg
     1981 ctgcacctgc tgtcgacccg cgttgtacga cgcgatggcc aggctgaggg agcctccgtt
     2041 gtcccgcaag agctccgcca gatgcatcgt cccgagctgg atgttcacct cgggcgactc
     2101 cagcggcggg gccgacggca gccggacctt gtatcgccgg gccacctgct ccgccgtctc
     2161 cggcatgagc tgcatcagcc cgcgggcgcc ggcccgagac acggctcgag gagcaaaggc
     2221 gctctcctcg cggatcaagg ccgtcaccag gtacggatcc agtccctgca gtgtcgcctg
     2281 ctggctcacc agggggaagt ggcccagggg gtacaggcac tgcca
//
LOCUS       DBNGZC010000007         8308 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_848, whole genome shotgun sequence.
ACCESSION   DBNGZC010000007 DBNGZC010000000
VERSION     DBNGZC010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 8308)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8308)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8308
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_848"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1233
                     /locus_tag="AC16G0_00080"
     CDS             130..1233
                     /locus_tag="AC16G0_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HIR5098045.1"
                     /translation="MKGINTMGGLGFSNNRFECSPDAERVGGERYKAEFHKKDTTCNR
                     CPVACGKTFEVKEGEYAGTVWKMPEYETIYAFGPMCGNLDPASLIKLEELCDQLGLDT
                     ISMGVTIAFTLECLEKGFLSEKEVGVPAGWGNHRAIQTLVEMTARRRGFGDILADGSA
                     RLAARLGPDAQKLLYAVKGLEVAGHSARALKGMSIGYATGTRGGSHHDTRPTPQYGPD
                     FDRRSPQGKPAWAIRSQHFTAVDDSLVLCRFTSERGGYGMYLNDHYVRMVNAATGWDL
                     DLPELERIGERICNLERALNVREGISRKDDTLPWRVQHEPIPDGPSKGMYCPPEELDR
                     MLDEYYDLRGWTPDGIPTPERLRDLGLGFAVRS"
     gene            1651..2856
                     /locus_tag="AC16G0_00085"
     CDS             1651..2856
                     /locus_tag="AC16G0_00085"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098046.1"
                     /translation="MRHRDWTWWAWIIGLGFLGLLLAGGPTQAQEYRIGFISAITGPA
                     SSLGVPERDVAVMLQKEIDAKGGITGPDGVKHPVKIIIHDDESKADTAARVAKKLIAE
                     DKVLVVIATSQSADSLAVVPVATEYKTPQVSMASSHAIVTPLKDRFWIFKTPQSNIHV
                     AFHQVKYLKAKKITKIASLYVNNAFGEDSRRGLAELMKGTDIQVVYEDKFEATDKDMT
                     PQLTKVKASGAQALLVHTTPPSASILTKNFRDLGLTIPLIHNQGVGNKPFIDLAGGAA
                     DGVIFPVGKMLVAETLPDADPQKKVLLDFIKAYESATGKPRSTFAGHGWDALQMSFMV
                     LAGLRANTPLEQARAEVRDGLEKIKNFAGTAGIFTMSAQDHVGLDERSMVLVRIEGGA
                     WKYFPPEKW"
     gene            2979..3824
                     /locus_tag="AC16G0_00090"
     CDS             2979..3824
                     /locus_tag="AC16G0_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5098047.1"
                     /translation="MTTGSIYALIALGFVTIYNVTGIINFAQGEFAMLGAMLAVSLLP
                     LGLPQPVTFLLAVAGVVVIGAGLERLAIHPARRASPVTLIIITIGADIAIRGIALLIW
                     GTDPYPLPAFTSGPPLMLDGAVLTRQAIWIMGTTAVSLALLAAFFERTYLGKAVRACA
                     INRLSARLTGIRPDRMSLLAFGLSAGLGAVGGIVIAPMTLVSYDMGLALGLRGFVAAI
                     MGGMVSAPGAVLGALLLGVLEALVAGLFRAAFKEAFAFLVLFLILVLRPQGLFGRGPA
                     PGAGL"
     gene            3824..4819
                     /locus_tag="AC16G0_00095"
     CDS             3824..4819
                     /locus_tag="AC16G0_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5098048.1"
                     /translation="MTALRRPWRITGLGGIVLALALIPVLDSIPSHLDVLIFIGIHTI
                     VTVGLCLLTGYAGQISLGQAAFYGLGAYASGLLTTKLEVPSLLALLAGVIVTASLAYL
                     LGIPILKLHGHYLAMATLAFGIIVYLAFVEFADLTGGPSGLPGVPPMRIAGFVFDTDL
                     KRYYLVLVAVLTVLLLAWNVVHSRVGRALRSIHGSEIAAETLGVDTTKYKLQVFALSA
                     AFASLAGSLYAHHLRLVSPSPFGFIGSVEFLVMAAVGGLASVWGAPFGAAAITLLAEG
                     IRRIVPHLFRYASGEHEVIAYGLLLMLIMIFMPEGLVSGTADLLRRRRGRTGGRG"
     gene            4823..5605
                     /locus_tag="AC16G0_00100"
     CDS             4823..5605
                     /locus_tag="AC16G0_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5098049.1"
                     /translation="MVAGSPVLETRGLCKAFGGVVAVANVNLAVREGQIHAVIGSNGA
                     GKTTLLNLISGLTPPDAGEIRFRGEPLAGRRPSDRAALGVARTFQNLQILGNMTVLEN
                     VMVGRHLKSNSGLMAAALRLRRAQQEERAIQREALRYLEFMGLAERRDDPAGSLPFGQ
                     QRWLEIARALATEPSLLLLDEPAAGLNPTETDCLGGLIQIIRNRGITVVLVEHDMDLV
                     MRVSDTVLVLNYGEVLAEGPPAEIQNNLAVVDAYLGSELDDA"
     gene            5598..6374
                     /locus_tag="AC16G0_00105"
     CDS             5598..6374
                     /locus_tag="AC16G0_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5098050.1"
                     /translation="MLEVADLHVAYGGIEALKGVTLSVKAGSITCLIGANGAGKTTLL
                     RAISGLLPIRGGRIQYAGQDITGLPAEDVVRLGVSQVPEGRGNFTSMSVRDNLLLGAY
                     RLPPATRRDGTEVDLQRIFELFPVLQSRQRQLAGTLSGGEQQMLAMGRVLMARPSVIL
                     LDEPSMGLAPLLVREILRTIQRLPARGTTPLLVEQNARAALRIADYGYVMENGRVVLQ
                     GSSRDLLEDPRVRRAYLGQDRTTSAGPVAIPWDLEAGDES"
     gene            complement(6498..7130)
                     /locus_tag="AC16G0_00110"
     CDS             complement(6498..7130)
                     /locus_tag="AC16G0_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5098051.1"
                     /translation="MIRILVADDHTVVRCGLRQIVSEEADMTVTGEACDSQELVALIR
                     EKPCDVLVLDISMPGRSGLEVLKDVKQEYPTLPILVLSVHPEDQYAVRTLKLGASGYL
                     TKDSAPEELVHAIRKVLGGRKYVSPALAEKLATNLITDSGRPPYETLSERERQVLCMI
                     ASGKSITEISDELAISVKTVSTYRTRVLEKMNMRNNAELTHYAIASRLVE"
     gene            complement(7127..>8308)
                     /locus_tag="AC16G0_00115"
     CDS             complement(7127..>8308)
                     /locus_tag="AC16G0_00115"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR5098052.1"
                     /translation="LDQLAHAFDHMAEILQQREAERQRAAATQARLTAILEATTDFVA
                     TADPEGQVLYCNRAARRMLGIGEGENIAGLRIPDTHPAWASAVVLTEGIPAAKRHGAW
                     SGETAFLSRDGREIPVSQVILAHASRSGQIEYFSTIARDISERKQAEEDLRKSREQLR
                     ALAAHLESVREEERSAIAKEVHDELGQGMTGLKIDLAWVASRLPEAKPALQEKVSETL
                     TRLDDMIRVVRRLTEELRPSLLDVLGLVEAIEWQAQEFRARTGLECAVAAEEGIPPLG
                     GAQATALFRICQEALTNVARHAHASRVTIRLEEDADHLTLAVEDNGKGITQQEIVKGS
                     SLGLLRMRERALLLSGGFAVVGRPGGGTTVTVRIPLGAQHLQDIGEQAETPAPLAGGK
                     S"
BASE COUNT         1343 a         2779 c         2696 g         1490 t
ORIGIN      
        1 ggctcgccgc ccgagaaagt cactccgccg ccggacgact cgtagaacgg cgtgtcgcgc
       61 acgacctccg ccatgacctc catgaagaat cgtacgtaca gcctccacgt cttcggcacc
      121 gcgaacctcg tgaaggggat taacaccatg ggcgggctgg gcttctcgaa caaccggttc
      181 gagtgctcgc ctgacgccga gcgggtgggc ggcgagcgct ataaggcgga attccacaag
      241 aaggacacca cctgcaaccg gtgcccggtg gcctgcggca agaccttcga ggtgaaggag
      301 ggcgagtacg ccgggacggt ctggaagatg ccggagtacg agaccatcta cgccttcggc
      361 cccatgtgcg ggaacctgga tcccgcctcc ctgatcaagc tggaggagct ttgcgaccag
      421 ctcggtctgg acaccatcag catgggggtg accatcgcct tcacgctgga gtgcctggag
      481 aaaggcttcc tgtccgagaa ggaggtgggc gtgccggctg gctggggaaa tcaccgggcc
      541 atccagacac tggtggagat gaccgcgcga cgccgtgggt tcggcgatat cctggctgac
      601 ggctccgccc ggcttgcggc acgactgggc cccgacgccc agaagttgct gtacgccgtc
      661 aaggggttgg aggtcgcagg gcactccgcc cgcgccctga agggcatgag catcgggtac
      721 gccaccggca cccggggtgg gagtcatcac gacacccggc cgacgcccca gtatgggccc
      781 gatttcgatc gtcggagccc gcagggcaag cccgcctggg ccatccgcag ccagcatttc
      841 accgccgtgg atgattccct ggtcctgtgt cgcttcacgt cggagcgggg cggctacggg
      901 atgtacctca acgaccacta cgtgcggatg gtcaacgcgg ccaccgggtg ggacctcgat
      961 ctcccggaac tggagcggat cggcgaacgc atctgcaatc tggagcgggc cttgaatgtc
     1021 cgcgagggga tctcgagaaa ggacgacacg cttccctggc gcgttcagca cgagccgatc
     1081 ccggatggcc cgtcgaaggg gatgtactgc ccgcctgaag agttggaccg gatgctcgac
     1141 gagtactacg accttcgcgg ctggacgcca gacggcatcc cgacgccgga gcgcctccgc
     1201 gatttgggac tgggtttcgc cgtccggtcc tgacctcacc cagagggctt tcccctggaa
     1261 cggtcctcgc gacaagtgct cggccggcat gaggacactt tcgtgtagcc acgcttaatc
     1321 tgttccggaa ggtggatggt gctgcctccc tgcttgtctt gacaggcagt taactcccca
     1381 aaaggacctt gacacgctgt ttgctcccaa atatattccg cgcacatttg cgatcggata
     1441 tggccaacct tttctggcac ttctcgccct ccgggatccc gcgttagccc gtcttgtggc
     1501 cgcaggcgcg gcgtccgact gttgtcggac gacatcttca ggggtgaggt cgaaaggcgc
     1561 cattgggcgc tgggccgacg agggaagggt cccgacaccg gagtgcctgg ggtgaatcat
     1621 tcgtacggtc ttgcacgagg gagggaaacc atgcggcatc gagattggac ctggtgggcg
     1681 tggatcatcg gcctgggctt cctggggttg ctcctggcag gcggcccgac gcaggcccag
     1741 gagtaccgga tcggcttcat ctctgccatc accgggccgg catcatctct cggggtcccg
     1801 gagcgtgatg tggccgtcat gctccagaaa gagatcgatg ccaagggggg catcaccgga
     1861 cccgatgggg taaagcaccc cgtcaagatc atcatccatg acgatgagtc caaggcagat
     1921 accgcggccc gcgtggcgaa gaagctcatc gccgaggaca aggtgctggt ggtcatcgcc
     1981 accagccaga gcgctgacag tctggccgtt gtcccggtgg ccaccgagta caagacgccc
     2041 caggtctcca tggcctccag ccacgccatc gtcacgccgc tcaaggaccg cttctggatc
     2101 ttcaagacgc cgcagtcaaa catccacgtc gcctttcatc aggtgaagta cctcaaggcc
     2161 aaaaagatca ccaagatcgc cagcctgtat gtgaacaacg ccttcggcga ggactcgcgc
     2221 cgaggtctgg cagagctcat gaagggcacc gacatccagg tcgtgtatga ggacaagttc
     2281 gaggcgaccg acaaggacat gaccccacag ctcaccaagg tcaaggccag cggggcccag
     2341 gccctgctcg tccacacgac tccgccgtcc gcctccatcc tcaccaagaa cttccgcgac
     2401 ctcggcctga ccattcccct gatccacaac cagggagtcg ggaacaagcc gttcatcgac
     2461 ctagccgggg gcgcggccga tggggtgatc ttccccgtcg ggaagatgct ggtggcggaa
     2521 accctgcccg acgccgatcc ccagaagaag gtgctcctgg atttcatcaa ggcctacgag
     2581 tccgcgacag gcaagccccg tagcactttc gccggtcacg gctgggatgc gctccagatg
     2641 tccttcatgg tccttgccgg cctccgggcc aacacgcccc tggagcaagc acgtgccgaa
     2701 gtgcgcgacg ggctcgagaa gatcaagaac ttcgccggga cggccggcat cttcaccatg
     2761 tcggcccaag accacgtggg tctcgacgag cgatccatgg tcctggtacg aatcgagggc
     2821 ggagcctgga agtattttcc ccccgagaag tggtgacagt gagggcatcg cggtagggcg
     2881 cgccgcgggg agacccggta gccacccgtc tccgcggcgc gtttccttgg aggaccgaca
     2941 ttcctgccgg ccaactgctc cagctcatcc tgagcggcct gacgacaggg agtatctacg
     3001 ccctgatcgc cttgggcttc gtcaccatct ataatgtcac cgggatcatc aacttcgccc
     3061 agggcgagtt tgccatgctg ggagccatgc tggcggtctc gctcctgccc cttgggcttc
     3121 cccaacccgt gacgttcttg ctcgccgtgg cgggcgtcgt ggtgatcggt gccggcctag
     3181 agcgcctggc catccacccg gcccgtcgcg cctcgcctgt cacgctcatc atcatcacca
     3241 tcggggccga tatcgccatc cgggggatcg cgctgctgat ctgggggacc gacccgtatc
     3301 ctctccccgc cttcacgtcc ggcccgccgc tgatgctgga cggggcggtg ctcacgcgtc
     3361 aggcgatctg gatcatggga acgacggccg tctccctcgc gctcctggcg gcgttcttcg
     3421 aacggaccta cctcggcaag gcggtgcggg cctgcgccat caaccgactt tccgcccgcc
     3481 tgacgggcat tcggccggac cggatgtccc tcctggcctt tgggctctcg gcggggctgg
     3541 gtgcggtggg gggcatcgtc atcgcgccca tgaccctggt gagctatgac atgggactcg
     3601 ccctcggcct ccgcgggttc gtggccgcca tcatgggcgg catggtcagc gcacccggcg
     3661 ccgtgctggg ggccctcctc ttgggcgtcc tggaggccct ggtcgcgggc ctcttccggg
     3721 ccgccttcaa ggaggcgttt gccttcctgg tgctgttcct gatcctggtc ctccggcccc
     3781 aggggctctt cggtcgcggg cctgccccgg gagcgggcct gtgatgacgg ccctgcgacg
     3841 gccgtggcgc atcacggggc tcgggggaat tgtgctcgcc ctcgccctga tccccgtcct
     3901 ggattcgatc ccctcgcacc tggacgtcct catcttcatc ggcatccaca ccatcgtcac
     3961 cgtcggcctg tgcctactga ccggctacgc ggggcagatc tccctggggc aggcggcctt
     4021 ctacggatta ggggcctacg cctcgggttt gctcacgacg aagctggagg tcccgtccct
     4081 tctggcgctc ttggccgggg tgatcgtcac ggcgtctttg gcctacctcc tggggattcc
     4141 catcctcaag cttcacggcc actacctggc catggccacc ctggccttcg ggatcatcgt
     4201 gtacctcgcc ttcgtcgagt tcgccgacct caccggcggc ccttccgggc tgccgggcgt
     4261 ccctcccatg cgcatcgccg gcttcgtctt cgacacggat cttaagcggt actacctcgt
     4321 cctggtcgcc gtcctgaccg tcctgctcct cgcctggaac gtggtgcact cccgcgtggg
     4381 gcgggctctc cgctccatcc acgggagcga gatcgccgcc gagaccttgg gcgtggacac
     4441 gacaaagtac aagctgcagg tcttcgccct gagcgcggcc ttcgccagcc tggccgggag
     4501 cctctacgcc caccacctgc ggctggtgag cccatcgcca ttcggcttca tcggctcggt
     4561 ggagttcctg gtcatggccg cggtgggggg actggccagc gtctggggcg cccccttcgg
     4621 ggccgccgcc atcaccctcc tggccgaagg catccggcgg atcgtgccgc acctcttccg
     4681 ctacgcttcg ggcgagcacg aggtcatcgc gtacgggctg ctcttgatgc tgatcatgat
     4741 cttcatgccg gaaggtctgg tctcggggac cgccgacctt ctgcgccgac ggcggggcag
     4801 gaccggcggg aggggctgag gcatggtcgc aggctcaccc gtgctcgaaa cgcggggtct
     4861 ctgcaaggca ttcggcggcg tcgtggccgt cgccaacgta aatctggcgg tccgggaggg
     4921 gcagatccat gccgtcatcg gatccaacgg cgccggcaag acgaccctcc tgaatctcat
     4981 cagtggtttg accccgccgg atgcgggaga gattcgattc cggggagagc cactcgcggg
     5041 acggcggcca tccgaccggg cagccctggg cgttgcccgg acctttcaaa acctccagat
     5101 ccttggcaac atgacggtgc tggagaacgt catggtgggc cgccacctga agagcaacag
     5161 cggcctcatg gcggcggccc tgcgccttcg ccgcgctcag caagaagagc gggccatcca
     5221 gcgggaggcc ctgcgatacc tggagttcat gggcctggcg gagcgccgcg acgatccggc
     5281 cggcagcctc cccttcggcc agcagcgctg gctggaaatc gccagggccc tggccacgga
     5341 gccttccctt ctcctgctgg acgagccggc cgcgggtctg aatcccacgg agaccgactg
     5401 cctgggcggg ctgatccaga tcatccggaa ccgtggaatc accgtggtcc tggtggaaca
     5461 cgacatggac ctggtcatgc gggtctcgga tacggtgctg gtcctgaact acggcgaggt
     5521 tctggcagaa ggaccgccgg ccgagatcca gaacaacctc gccgtggtgg atgcttacct
     5581 ggggagcgag ctggacgatg cttgaggtgg cagatctgca cgtggcctat ggcgggatcg
     5641 aggcgctgaa gggggtcacg ctcagcgtca aggcaggaag catcacctgc ctgatcggcg
     5701 ccaacggggc cggcaaaacg accctgctcc gggccatctc cggtctgctc ccgatccgtg
     5761 ggggccgcat ccagtatgcg ggacaggaca tcaccggact ccccgccgag gacgtcgtcc
     5821 gtctcggcgt cagccaagtc ccggaaggcc gtgggaattt caccagcatg agcgtgcggg
     5881 acaatctcct gctgggggca taccgcctcc cgcccgccac ccggcgggac ggaacggagg
     5941 tcgatctcca gcggatcttc gagctttttc ccgtcctgca gtcacgacag cggcaactgg
     6001 cgggaaccct gtccggcggg gagcagcaga tgctggccat ggggcgcgtc ctcatggccc
     6061 ggcccagcgt gatcttgctg gacgaaccct ccatgggtct cgcccccctt ctggtccgcg
     6121 agattcttcg cacgatccag cggctgcccg cccgggggac aacgccactc ctggtggagc
     6181 agaacgctcg ggcggccctt cggatcgcgg attacggcta cgtgatggag aacgggcggg
     6241 tcgtgctgca gggatcgtcc cgggacctcc tggaggaccc gcgggtgcgt cgggcctacc
     6301 tcggccagga tcggacgacc tctgccggac cggtcgcgat cccctgggac ctcgaggcag
     6361 gagacgaatc atgaccacag gcggcgcgag tctagcctgg agttgttgga acagggccat
     6421 ggcaggaaga gggcaggcgc cagagcctgg agggagtctg ggggcaccac gggagacgcg
     6481 accgatctca gaccacgtca ctccaccagc cggctggcga tcgcgtagtg ggtgagctcg
     6541 gcgttgttcc gcatgttcat cttttccagg acccgcgtcc ggtatgtgct gacggttttg
     6601 acgctgatgg cgagctcgtc gctgatctcc gtgatggatt ttccggaggc gatcatgcaa
     6661 agaacctggc gctcgcgctc ggagagggtc tcgtaaggcg ggcgtccgct atcggtgatt
     6721 aagtttgttg ccagcttctc cgcaagcgcg gggctgacgt atttccttcc ccccaagacc
     6781 ttccggatcg cgtggaccag ttcttccggc gcgctgtctt tggtcaggta ccccgacgcg
     6841 ccaagcttca gggtgcgtac cgcgtactga tcctcgggat gcacgctcaa gaccaggatc
     6901 ggcagggtcg gatactcctg tttcacgtcc ttcaggactt ccaggccgct cctgccgggc
     6961 atggagatgt ccaacaccag gacgtcgcac ggcttctctc ggatgagcgc aaccagctcc
     7021 tgggaatcgc aggcctcgcc ggtcaccgtc atatcggctt cttcgctcac aatctgcctc
     7081 agcccgcagc gcacgacggt gtgatcatcc gcgaccagaa tgcgaatcat gacttgcctc
     7141 cggctagggg agcgggcgtc tcggcctgct ccccgatgtc ctgaagatgc tgcgccccga
     7201 gggggatccg gaccgtcacc gtggtgccgc cgcccggccg acccacgacg gcgaaccccc
     7261 cgctcaggag gagagcccgt tcgcgcatgc gcagcagccc gagagaggat cccttgacga
     7321 tctcctgctg ggtaatccct ttgccgttgt cctctacggc cagggtgaga tgatccgcgt
     7381 cctcctccaa ccggatggtc actcgcgagg catgggcgtg acgggcgaca ttggtcaggg
     7441 cctcctggca gatgcggaaa agcgccgttg cctgggctcc ccccaggggc ggaatcccct
     7501 cctccgctgc gaccgcacac tcgaggcccg tgcgggcgcg gaactcctgc gcctgccatt
     7561 cgatggcctc caccaggccg agcacatcca ggaggctggg acgcaactcc tcggtgagcc
     7621 ggcggaccac gcgaatcatg tcgtccagcc gtgtgagtgt ctccgagacc ttttcttgca
     7681 gggccggctt cgcctcgggc agacgagacg ccacccacgc gagatcgatt ttcagccccg
     7741 tcatcccctg ccccagctca tcgtggacct ctttggcgat ggcggaccgc tcctcctcgc
     7801 gtacggattc cagatgggcg gccagggccc ggagctgctc tcgggacttg cggaggtctt
     7861 cctccgcctg cttgcgctcg ctgatgtctc gcgcgatggt ggagaaatac tcgatctggc
     7921 cgcttcggga cgcatgtgcc aggatcacct gcgacactgg gatctcgcga ccgtcccggc
     7981 tcaggaaggc cgtttccccg ctccaggcgc cgtgccgctt ggctgccggg atcccctccg
     8041 tcaggacgac cgcactcgcc caggcgggat gggtgtcggg aatccggagc ccggcgatat
     8101 tctccccctc gccaatgccg agcatccgcc gggctgctcg gttgcagtag agcacctgtc
     8161 cctccgggtc cgcggtggcc acaaagtcag tggtcgcctc cagaatggcg gtcaggcggg
     8221 cctgggtggc tgccgcccgc tggcgttccg cctctcgctg ctgaagaatt tcggccatgt
     8281 ggtcaaaggc atgggcgagt tggtccaa
//
LOCUS       DBNGZC010000008         3279 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1761888, whole genome shotgun sequence.
ACCESSION   DBNGZC010000008 DBNGZC010000000
VERSION     DBNGZC010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1761888"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="AC16G0_00120"
     CDS             <1..628
                     /locus_tag="AC16G0_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5098053.1"
                     /translation="TSLEKAMRSHFQTHRHVSYLFAGSKESTLRDMAARERSPFFKFG
                     RLVPLGPIPIAEFAPYLEERFRRGRLRVSREILDAVLAAADDVPYNVQRLCHELWALR
                     AGSANRIVEQDIGAAILRIVEQDASHFSAMWDRLSLHQRQVLQAIARSGGRNIFAADF
                     LASHRLGSHSSVQTSLRMLLKDQILFRTNGEYRFADAFFREWIATRLP"
     gene            complement(852..1328)
                     /locus_tag="AC16G0_00125"
     CDS             complement(852..1328)
                     /locus_tag="AC16G0_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098054.1"
                     /translation="MMLRKILGSLLVLLVASQASAAELRVTLVPDLSDEKVWQFEVIN
                     DSVDILDVVRLTATFYANGRRLWSAPVVLTPSLLRPGEPGWVALDARMVPKQSPLRID
                     WELTWNPHSVPVLPQFWKTEHVASLEIRPVSPSTGAAQAPDKQSPEPEPKDPTWRF"
     gene            1630..3210
                     /locus_tag="AC16G0_00130"
     CDS             1630..3210
                     /locus_tag="AC16G0_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098055.1"
                     /translation="MRKCIGALAVLMGIFVLALPAWAGTPRSGGTLVVAIGADGTGLD
                     PRTVMNNESGFVMSAVLEGLTRYKLGTTEVEPALSEGWTVSSDGKTITFRLRKGVKFH
                     DGTPFDADAVIFDFDRILNEKSQYYYRAGVTAGSFVPEFYGEVAGYQKVDQYTVRVTL
                     KRKFAPFLDNLATAFSGIVSPAAVKQWGPVEVAKHPVGTGPFKFVEWVRNDHMTVEVN
                     PDYWGGKPYLDRIVYRIIPENSVRLLNLEQGSVDIVDGINPDDVQRIKANSKLVLLEQ
                     AGATINGIAMNNQKAPFDNPKVRQAMNYAVNREEINNFLYKGVAVLSTNPVPPVTWSF
                     DKSLTGYPYDPAKAKQLLAEAGHPNGFKVEMIAFPNPRGYNPVGGARLAVAVQDYLRR
                     VGVEVSIKQLEWGTFLKTIRSGDYLIGPAGWSADNGDPDNFLFSLYSSTTWGSGNNAR
                     YKNPEVDKLLQEAQETFDREQRMQLYRKAEKMIMDDAAWIFINHTKLVRATTARVQGY
                     VLNPTQMFFHMNQVWVTQ"
BASE COUNT          604 a         1065 c         1025 g          585 t
ORIGIN      
        1 caccagcctc gaaaaggcca tgcggtccca ttttcaaacc catcggcacg tgagctacct
       61 gtttgccggc tcgaaggaaa gcacgctccg cgacatggcg gcccgcgagc gaagcccctt
      121 cttcaaattc ggccggcttg tccccctcgg ccccattccg atcgcggaat tcgccccgta
      181 tctcgaggag cgctttcgcc gcggccgtct ccgggtgtcc cgggagatcc tggatgccgt
      241 cctggcggcg gcagacgacg tcccctataa cgtgcaacga ctctgccatg aactctgggc
      301 gttgcgcgcg gggtcggcga atcggatcgt cgagcaggac atcggcgctg cgatcctgcg
      361 gattgtcgag caggatgcgt cccacttcag cgccatgtgg gatcgccttt cgctccatca
      421 gcggcaggtc ctgcaggcga tcgctcgcag cgggggccgg aatatcttcg ccgccgattt
      481 cctcgcgtcc caccgcctgg gctcccactc ctcggtccag acctcgctgc gcatgctctt
      541 gaaggatcag attcttttca ggacaaacgg cgagtaccgg ttcgccgatg ccttcttccg
      601 ggaatggatc gccacccggc tgccctgagt ccgaacaaat cagttggacc gcagggcgca
      661 ccgagcacgc ggagaggacg actggacttc agtggatgcc gcagcctggc cggcaaggat
      721 tccaagatac ttcgagagag aatcctcccg cgtcggctgc ctcggcgtgc cttgcgggcc
      781 tggcggtcat ggaattgggt tgagtcctgc gtctgcagac acgccccggg cgtttttcgt
      841 gaactccgct ctcagaaccg ccaggtgggg tccttgggtt ccggctccgg gctctgcttg
      901 tctggagctt gggccgcccc ggtggagggg gacaccgggc ggatctcgag gctggccaca
      961 tgctcggtct tccagaactg cgggaggacc gggacggagt gtgggttcca ggtcagctcc
     1021 cagtcgatgc gaagcggcga ttgcttgggg accatccgtg cgtccagcgc cacccagccg
     1081 ggctcccccg gccgcagcag cgatggcgtg agcaccacgg gcgcggacca gagccgacgg
     1141 ccgttcgcat agaaggtcgc ggtgagccgc acgacatcca ggatgtccac ggagtcgttg
     1201 atcacctcga actgccacac cttttcatcg gacaggtcag gaaccagcgt cacgcggagc
     1261 tcggcggccg aggcttgact cgctaccaac agaactagaa gcgatcccag aatctttcgc
     1321 agcatcatgt tccccccctc ctgtctatcg cctcccaaag tctagcatgc caagatcgat
     1381 ctggggatgg ggggagacgt ttccccgtgc cgccacggaa cacgctcggg ccaccagcct
     1441 acagtcgccc acctgtatag cggggtgaac cgccctgaac gcgatcgggc ttgacaccct
     1501 tcccggaccc ggaatacatt tgcgcacttt catccagggt tgcttgtggt tcttcgttgg
     1561 gttgatccag gagccgggcg tcttccccgg gctggacctg cgggaggcac ggcagagagg
     1621 gaggatgtca tgcgtaagtg catcggcgca ctggctgtac tcatgggaat cttcgtcctg
     1681 gcgcttcccg cctgggccgg gacaccccga tcggggggaa ccctggtcgt ggctatcggg
     1741 gccgacggga ccggcctgga tccccgtacc gtgatgaaca acgaatcagg gttcgtgatg
     1801 tccgccgtcc tggaaggcct cacccgatac aagctcggga cgacggaggt cgagcctgcc
     1861 ctgtccgagg gctggaccgt gtcctcggac gggaagacga tcacctttcg cctgcgcaag
     1921 ggcgtgaagt tccacgatgg aacccccttc gacgcggatg cggtgatctt cgacttcgac
     1981 cggatcttga acgagaaaag ccagtattac taccgcgccg gcgtgacggc cggcagcttc
     2041 gtcccggagt tctacggcga agtggcgggc taccagaagg tggatcagta caccgtcagg
     2101 gtcacgctca agcggaaatt cgccccgttc ctggacaacc tggccaccgc cttctccggt
     2161 atcgtaagcc cggcggccgt gaagcagtgg ggtccggtgg aagtcgccaa gcacccggtg
     2221 gggaccgggc ccttcaagtt cgtggagtgg gtccggaacg atcacatgac ggtcgaggtc
     2281 aacccggact actggggcgg caagccctac ctcgaccgaa tcgtctaccg gatcatcccc
     2341 gagaactcgg tccgcctgct gaacctggag caggggagcg tggacatcgt ggacgggatc
     2401 aatccggacg acgtccagcg gatcaaggcc aacagcaaac tcgtcctcct ggagcaggcg
     2461 ggggcaacga tcaatggcat cgccatgaac aaccagaagg cccccttcga caaccccaag
     2521 gttcggcagg ccatgaacta cgccgtgaac cgggaggaga tcaacaactt cctctacaag
     2581 ggggtggccg tcctctccac caacccggtg ccgcccgtca cgtggtcctt cgacaagagc
     2641 ctgacggggt atccctatga tccggccaag gccaagcaac tcctggccga ggccggtcac
     2701 cccaacggct tcaaggtcga gatgatcgct ttccccaacc cccgcggcta caacccggta
     2761 ggtggcgccc ggctcgccgt ggccgtccag gattacctgc gccgcgtggg ggtggaggtg
     2821 agcatcaagc agcttgagtg ggggaccttc ctcaagacca tccggtcggg cgattacctc
     2881 atcggccccg cgggctggtc ggcggacaat ggcgatccgg acaacttcct gttcagcctg
     2941 tacagctcca ccacctgggg ctcgggcaac aacgcccgct acaagaaccc cgaggtggac
     3001 aagctccttc aagaggctca ggagaccttc gaccgcgagc agcgcatgca gctttaccgg
     3061 aaggccgaga agatgatcat ggatgatgct gcctggatct tcatcaacca caccaagttg
     3121 gtgcgcgcca ccacggcgcg ggtccaggga tacgtcttga acccgaccca gatgttcttc
     3181 cacatgaacc aggtgtgggt aacccagtaa gcgaggtcga acgatcgtcg ccctcgtcct
     3241 ccgtcgtttg ctccataccg ggcctgtcct ggtggcggc
//
LOCUS       DBNGZC010000009         3424 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1664099, whole genome shotgun sequence.
ACCESSION   DBNGZC010000009 DBNGZC010000000
VERSION     DBNGZC010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1664099"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="AC16G0_00135"
     CDS             complement(<1..863)
                     /locus_tag="AC16G0_00135"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006696992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5098056.1"
                     /translation="MAAGIDIRRVRKSFGSVVAVRDVSLTVESGEFFTLLGPSGCGKT
                     TLLRTVAGFCPLDGGEIWVGGRRVDRIPPHRRNTGMVFQNYAVFPFLNVFDNVAYGLR
                     ARGMPRHAIDERVGRCLEMVQLTEYAPRMPHQLSGGQLQRVAIARCLAIEPEVLLLDE
                     PLSNLDAKLRVQMRSEIRRLQQALKITALYVTHDQEEALVLSDRLAVMSGGTIQQLGR
                     PSEVYHQPANAFTANFMGSTNLLRGRVVRHAQNRTGVAVGGQEILLPQVARPAEAEVL
                     FCLRPEAIRLAP"
     gene            complement(1069..2094)
                     /locus_tag="AC16G0_00140"
     CDS             complement(1069..2094)
                     /locus_tag="AC16G0_00140"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5098057.1"
                     /translation="MKWVRWSAIGVGFLLAGCIALVTVSPVFSADTSLVVYSANDTDV
                     NNFVFGMFQKETGITVQPVTAGSGVLLKRMAAEKDRPLGDVLWGVSTVILDANKAYFE
                     PYASKNIEAVPATIRDPQNLWAATNAHVIVIQINRKLLGSMASPRTWRDLQDPQWKGK
                     VVLADPANSGGAFTQVTRMLDLFGGGDTEKGWQELEKVIRNTRVLNKISLVYTGVGNG
                     EYPVGLSLEYAAYEYVQGGAPVELVYPEDGTVLQPEGMGIMKGAQHREAAQKFEDFIT
                     RKDVCEAILKKFFRRPARTDLDFAALGIKLPPLKDIKFASYNPAAWAKDRDAVLKHVQ
                     EIILKTR"
     gene            2516..2773
                     /locus_tag="AC16G0_00145"
     CDS             2516..2773
                     /locus_tag="AC16G0_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098058.1"
                     /translation="MESQLTIRLPEALHQRLDRIAKRLDRRRSDLARLAIQRYLDEIE
                     GESQPRPYARLHDLLGSLDSGVSDLGSRHRKHLLANFRRRG"
     gene            2766..3176
                     /locus_tag="AC16G0_00150"
     CDS             2766..3176
                     /locus_tag="AC16G0_00150"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR5098059.1"
                     /translation="MVDLLLDTGAWVALLDRREREHVRCVEALRGLTGRLISTEAVLT
                     ETLWLVSGLRDGPRRCAEFVQRGAVTVVPVSSESLARAVDLMEQYRNVPMDYADATLV
                     TLGEELDLDTVFTLDRRGFGVYRLRGRRSFQIVP"
BASE COUNT          596 a         1156 c         1054 g          618 t
ORIGIN      
        1 ggcgccaacc ggattgcctc cggccggaga cagaagagaa cttcggcctc ggcaggccgg
       61 gcgacctgcg gcaggaggat ctcctgcccg ccgaccgcca cgcccgtccg gttctgcgcg
      121 tgccggacca cgcggcctcg cagcaggttc gtggacccca tgaaattggc cgtgaacgca
      181 ttcgccggct ggtggtacac ctcgctgggc cgccccagtt gctggatggt gccgccggac
      241 atgacggcca gacgatccga caggaccagc gcctcttcct gatcgtgggt gacgtacagg
      301 gccgtgatct tcagcgcctg ctgcagcctg cggatctcgg agcgcatctg cacccggagt
      361 ttggcgtcca ggttggagag gggctcatcc aggagcagca cctcgggctc gatggcgagg
      421 caccgtgcaa tggccacccg ctggagctgc ccgcccgaca gttggtgcgg catccggggg
      481 gcgtactccg tgagctggac catctccagg catcgcccga cccgctcgtc aatggcgtgc
      541 cgcggcatcc cgcgggcccg gaggccgtac gccacgttgt cgaagacatt gaggaagggg
      601 aagacggcgt agttctgaaa gaccatcccg gtgttgcggc ggtgcggcgg gatccgatcc
      661 acgcgccggc cgcccaccca gatctcaccg ccgtccagcg gacagaaccc ggccaccgtc
      721 cggaggagcg tcgtcttgcc gcacccgctg ggcccgagga gcgtgaagaa ctcccccgac
      781 tccacggtca ggctcacgtc gcggacggcc accaccgagc caaagctctt ccgcacccgc
      841 cggatgtcaa ttcccgccgc catgcagctc cctcgcctcg cgtgcgttgg ccaaccgtct
      901 gcgctctaag atcggcagtc agctttaaat ccttcgattc ccccgcattc gcagaggcgc
      961 ggaggcgata aggcgcagag gtgaccacgc ttccctgccc tccctgcgcc tctgcgcctc
     1021 tgcgtccccg cgctctccga cgctgaccgc tggtcactga cggctgtact accgagtctt
     1081 gaggataatc tcctggacgt gcttgaggac cgcatcccgg tccttggccc atgccgcagg
     1141 attgtaagag gcaaatttga tgtctttgag gggcggcagc ttgatgccca gcgccgcaaa
     1201 gtccagatcc gtgcgcgccg gacggcggaa gaacttcttg aggatcgcct cgcacacgtc
     1261 cttccgggtg atgaaatcct cgaacttctg ggccgcctcg cgatgctggg cgcccttcat
     1321 gatccccatg ccctcgggct gcaggacggt gccgtcctcg gggtacacga gctccactgg
     1381 tgcgccgccc tgaacgtact cgtaggccgc atactccaga ctcaggccga cggggtattc
     1441 cccgttcccc accccggtgt agaccaggga gatcttgttc aggacgcggg tgttccggat
     1501 gaccttctcc agttcctgcc agcccttctc cgtgtctccg cccccgaaga ggtcgagcat
     1561 gcgggtcacc tgggtgaagg ctccgccgga gttggccgga tccgccaaga ccaccttccc
     1621 tttccactgg ggatcctgca ggtcccgcca agtccgagga gaggccatgc tccccaggag
     1681 ctttcggttg atctgaatca cgatcacgtg ggcattggtc gcggcccaaa gattctgggg
     1741 gtcgcggatc gtcgccggca cggcctcgat attcttcgag gcgtagggct cgaagtaggc
     1801 cttgttggcg tccaggatga ccgtgctcac gccccagagc acatccccca ggggccggtc
     1861 tttctcggcg gccatgcgct tcagcaggac gccagagccg gcggtcaccg gctggacggt
     1921 gataccggtc tccttctgaa acatcccgaa gacgaagttg ttgacgtcgg tgtcattggc
     1981 cgaatagacg accaggctgg tgtccgccga gaagacaggg ctcaccgtga ccaacgcgat
     2041 gcatcctgcc agcagaaatc ctacgccgat cgcgctccac cgcacccact tcatacccca
     2101 cctccctttc gttggacaga tggcctgccc aggacctccg ccatcggcga aggtccgatc
     2161 gctatttccg ggagacgcct cccatatgag ccgacgcgag ggtagaccgg ctcctgccag
     2221 ggctccccct ccgcgcccca tcccagatgg caagcacagc gcatccaacg ggcgagatcg
     2281 gctgaaacgc cgagatgccc cggaacatgc cgcacgagaa ccggacgtgt caagtcaaaa
     2341 acggtccggt attcgcccgg tggaatggtc gggcgggcca tcggatgccc gcggcagaag
     2401 aaccacgttc ctggcaacgg ggaatccgaa acggctgtcc gtctcagcgt acccttgcgt
     2461 acgcagtgca tatgcaatat aatgcattgc aatgtaaacc atccaggagg atgccatgga
     2521 atctcagctc accatccggc tgccggaagc cttacaccag cggctggacc ggatcgcgaa
     2581 gcgcttggac cggcggcgct cagatcttgc ccggctcgcc attcaacgct atctggacga
     2641 aatagagggc gaatcgcagc ctcgccccta cgcgcgtctc cacgatctcc tcggatccct
     2701 ggacagcggc gtctcggatc ttgggtcccg acatcgcaag caccttctgg ccaactttcg
     2761 ccgtcgtggt tgacctcttg ctcgacaccg gggcgtgggt ggccctcctg gatcgtcgcg
     2821 agcgggagca tgtccgctgc gtagaagcac ttcgagggct caccggccgc ctcatatcga
     2881 ccgaggcagt cctgaccgag accctgtggt tggtgtccgg actgcgcgac ggaccccgac
     2941 gctgcgcgga gttcgtccag cgcggcgccg ttacggtcgt gccagtttcc tcggagagtc
     3001 ttgcgcgggc tgtggatttg atggagcagt atcgaaacgt gccgatggac tatgcggacg
     3061 caacgttggt gactctcggc gaggaactag acttggacac cgttttcacc ctcgaccgtc
     3121 gcggattcgg cgtgtaccgc ctccgcggtc gccggtcctt ccagattgtt ccctgagtct
     3181 gtccatggtg tacgggcacg atcatcccga atccagctct cgccggaagg cgggtcctcg
     3241 cttctccgag gtatccagag ggacgcgcca ctgcgattcc gatgcagcca ttgaccgagc
     3301 gccatgtgac gtgctagggt gaacgcggtg atgaggaaca tggtcacgcc ggcaccggta
     3361 aaggttacct tggaggagta cctcgccctc cccgaaacca cactccccca tgagctcgtg
     3421 gatg
//
LOCUS       DBNGZC010000010         2430 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1077255, whole genome shotgun sequence.
ACCESSION   DBNGZC010000010 DBNGZC010000000
VERSION     DBNGZC010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1077255"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..954)
                     /gene="istB"
                     /locus_tag="AC16G0_00155"
     CDS             complement(196..954)
                     /gene="istB"
                     /locus_tag="AC16G0_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007505752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HIR5098060.1"
                     /translation="MAIQLLKAHLKQLRLPAMGLEFEKLAREAAASNQSYEQFLLKLT
                     EIELAARAANALAARIKQADFPVLKDFDTYDFSVMPQLSKPKVLELARGEWIEQKFNC
                     CFIGSPGTGKTHLALALGQAVCRAGYRVRFFTAAALVSCLEQAQKQYTLERFLKQLDR
                     ADLLICDELGYVSFSRAGVELLFRVFTDRYERASLLITGNLAFGDWGQVFQGERMTAA
                     LLDRITHRCHIFEMNGESYRFRESMKAKKGRQSD"
     gene            complement(1042..>2430)
                     /gene="istA"
                     /locus_tag="AC16G0_00160"
     CDS             complement(1042..>2430)
                     /gene="istA"
                     /locus_tag="AC16G0_00160"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HIR5098061.1"
                     /translation="PPKQRHTAMQIFRRRRDEHGYTGSYGQVRRYRSAHRRQHRETFI
                     PLAHPPGHRLEADFGHIHVDFPDGRRRVPVLVTVWAYSNYPFILALPTERTEAILAGL
                     VAAFDFFACAPREVWWDNPRTVATLIFQGRQRQLHPRYAALASHYAFDPLFCLPAHGN
                     EKPDAESTVKAVQRRFATPVPRVTDLDTLNAQFRQLCLAEQERIVQSLSGPFLIAARF
                     AEERTAAGRLPSRPFDPCIVRPAVQVDKYQTVTFDNNRYSVPRAYAFGPVTVKGYVER
                     VVVVADGRVVASHPRCYGRCPPVLDPLHYLATLGRKPAALDHAPVYRDWKLPVAFTEL
                     RAALEERHGALAGARQFIRVLELLRVHPQARVERAIVACRGEQVTTVEAILQRTRTLA
                     AQDQERGCQETCKCELATVPEIRVPRPDLGRFNQLLSGPGSGGAPGVERGDRGGEPCQ
                     EEGKSYDQDQIL"
BASE COUNT          449 a          792 c          786 g          403 t
ORIGIN      
        1 aagctggaag agaagcaagg aatgcctcca aagagatgcg agcagtgaat caacccggag
       61 gggagataac ccatctacgc tcttggatca tcaagagtgc gggagaaccg taaaacggac
      121 ccaccctgag cgcctaaaac ggtcccacct cgactttcaa aatagccgcc ctgggcaatc
      181 gcggcaacgc ttccctcagt ctgactgtcg acccttcttg gccttcatcg actcccgaaa
      241 ccgatagctc tcgccgttca tctcgaagat gtggcagcga tgcgtgatcc ggtcgagcaa
      301 cgccgccgtc atccgctcgc cctggaacac ctggccccaa tcgccgaatg ccaggttacc
      361 ggtgatcagc agactcgctc gttcatagcg atcggtgaac acccgaaaga gcaactctac
      421 cccggcgcgg ctgaacgata cgtagcccag ctcgtcacag atcagcagat ccgcccggtc
      481 cagttgtttc aggaaccgct ccagcgtgta ttgcttctgc gcctgttcca gacagctgac
      541 cagcgccgcc gctgtgaaga accgcacccg gtagcccgca cggcacaccg cctggcccaa
      601 ggccagcgcc aagtgggtct tccccgtgcc aggactccca atgaagcaac aattgaactt
      661 ctgttcgatc cactcacctc gcgccagctc caggaccttc ggcttggaca gctgcggcat
      721 cacgctgaag tcgtaggtgt caaagtcctt caggaccggg aaatcggcct gcttgatccg
      781 cgccgccagg gcgttggccg cacgggccgc cagctcgatc tcagtaagct tgagcaagaa
      841 ctgttcataa ctctggttgg aagccgcggc ctcacgcgcc agcttctcga actccaggcc
      901 catcgccggc agacgcagct gcttgagatg ggccttcagc aactgaatgg ccacctcatc
      961 ggtcttcgga ccactccttt cccagggaca tcgcggggtg gaaccgggcc tgaccaccag
     1021 gcccggtcac gagacgtatg gtcacagaat ttggtcttga tcgtatgatt tcccttcttc
     1081 ctgacagggt tccccgcccc gatcgccacg ctcgactccg ggcgcccctc cagaccccgg
     1141 accgctcagg agttggttga atcggcccag gtcgggccga ggcacacgaa tctcaggaac
     1201 agttgccagc tcacatttgc atgtttcctg acatccacgt tcctggtctt gagcggccag
     1261 cgtccgggtc cgctggagga tcgcctcgac cgtggtgacc tgttctccgc gacacgccac
     1321 gatcgcccgc tccacccgcg cctgggggtg gacgcgcaac agctccagga cccggatgaa
     1381 ctgccgcgcg ccggccaacg ccccgtgccg ctcctccaac gcggcacgca gttcagtgaa
     1441 ggcgacgggc agtttccagt cgcggtagac cggcgcgtgg tccagcgccg cgggcttgcg
     1501 gcccagcgtg gccaggtaat ggagcgggtc gagcactggt gggcagcgtc cgtaacaccg
     1561 ggggtgcgaa gccacgaccc ggccgtccgc caccaccacg acgcgctcga catagccttt
     1621 gaccgtcacc ggcccgaacg cataagcccg cgggacgctg taccggttgt tgtcgaaggt
     1681 gacggtctgg tacttgtcca cctggacggc gggccgcacg atgcatggat cgaagggccg
     1741 actgggcagc cgccccgccg cggtccgctc ctcggcgaag cgggccgcga tcaggaacgg
     1801 cccggacaac gactgcacga tgcgctcttg ctcggccagg cagagttgcc ggaactgggc
     1861 gttgagcgtg tcgagatcgg tgacccgcgg caccggggtg gcgaagcggc gctggacggc
     1921 cttgaccgtg gactcggcat cgggcttctc gttgccgtgc gccggcagac agaagagcgg
     1981 gtcgaaggcg tagtgactgg ccagcgcagc gtagcgggga tggagctggc gctggcggcc
     2041 ctggaagatc aacgtggcga ccgtcctggg gttgtcccac cacacttcgc gaggcgcaca
     2101 ggcgaagaaa tcgaaggcgg cgaccagtcc cgccaggatg gcctcggtgc gttcggtggg
     2161 cagggccagg atgaaggggt agttggagta ggcccagacg gtgaccagga cggggacccg
     2221 gcgtcgtccg tcggggaaat cgacgtggat gtggccgaag tcggcctcga ggcgatggcc
     2281 cgggggatgg gccagcggga tgaacgtctc gcggtgttgc cgccgatggg cggaccgata
     2341 gcgccggacc tggccgtacg agccggtgta gccgtgctcg tcgcggcggc ggcggaagat
     2401 ctgcatggcg gtgtgccgct gcttgggagg
//
LOCUS       DBNGZC010000011         1272 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1566478, whole genome shotgun sequence.
ACCESSION   DBNGZC010000011 DBNGZC010000000
VERSION     DBNGZC010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1566478"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1217)
                     /locus_tag="AC16G0_00165"
     CDS             complement(join(85..779,779..1217))
                     /locus_tag="AC16G0_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611506.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HIR5098062.1"
                     /translation="MEKYRVTLTLEERAALEQLVSKGKAAARKLTHARVLLLADDARG
                     EECPDDEIVATLGISPRTVARVRKRLVTEGFQAALDHRPQPARPDKIQIGGDVEQKLV
                     KLACADPPRGRCRWTLQLLADEMVVLGLVDAISLETVRQALKKTNIQPWIVQTWCLPP
                     DADAEFVWRMEDVIQTYRLPYDPRYPVVCFDEVCKQLFGEVRPPRGARRGKPARVDYE
                     YERKGVCHQFLMCEPLRGWRHVELTERRTRRDYARCVRDLVDRDYPRARMVRLVQDNL
                     NTHDGASLYEAFAPEEARRLLDRIEFHYTPKHGSWLNMAETEISIMDRQCLDRRLDGQ
                     GRMAEEDAAWEHGRNAREARIHWTFTLAAARRKLRKLYPSIDV"
BASE COUNT          188 a          447 c          403 g          234 t
ORIGIN      
        1 gacccagcaa ccggactact tggagcaggt ctgggtggcg tgtggcgcag ccgatcggcc
       61 ccgcttacgg cgtctagtgg gccttcaaac gtcgattgac gggtagagct tccggagttt
      121 ccgccgcgcg gccgccaggg tgaaggtcca gtggatccgt gcctcgcgag cgttccgccc
      181 gtgctcccaa gccgcgtcct cctcggccat tcgaccctgg ccgtccaacc gccggtccag
      241 gcattggcgg tccatgatgc tgatctccgt ctccgccatg ttcagccaac tgccgtgctt
      301 gggcgtgtag tggaactcga tccggtccaa gagccgacgg gcttcctccg gagcgaaggc
      361 ttcgtacagg ctcgccccgt cgtgcgtgtt caggttgtct tgcaccaacc ggaccatcct
      421 cgctcggggg taatcccgat ccaccaggtc ccgcacgcag cgggcatagt cccgccgggt
      481 gcgccgctcc gtcagctcga catgacgcca gccccggagc ggctcacaca tcaggaactg
      541 gtggcacacc cctttccgct cgtactcgta gtccacccgg gcgggcttgc cccgtcgcgc
      601 cccgcggggc ggccgcacct cgccgaagag ctgcttgcac acctcgtcga agcagaccac
      661 cgggtacctc gggtcgtagg gcaatcggta ggtctggatc acgtcctcca tccgccagac
      721 gaactcggca tcggcgtccg gcgggagaca ccaggtctgg acgatccacg gctggatgtc
      781 gtttttttca gcgcctggcg caccgtctcg aggctgatcg catcgaccag gccgagcacg
      841 accatctcat cggccagcag ttgcaaggtc caacggcacc ggccccgggg tgggtcggcg
      901 cacgccagtt tgacgagctt ctgttcgacg tcccccccga tctggatctt gtccggcctg
      961 gccggctgag ggcggtgatc gagggccgcc tggaatccct cagtgacgag ccgcttccgg
     1021 acccgggcca cggtccgcgg gctgatgccc agggtggcga cgatctcgtc atcggggcat
     1081 tcctcccccc gggcgtcgtc ggccagcagg aggacgcgcg catgggtcag cttgcgagcc
     1141 gcggcctttc ccttggaaac cagttgctcc aacgctgccc gctcctccag ggtcaaggta
     1201 actcgatact tctccatcgg tgcactcctg tcgttgaggg ccgtctcgtg gttcgatgcc
     1261 tcaacggaca gg
//
LOCUS       DBNGZC010000012         1678 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2642710, whole genome shotgun sequence.
ACCESSION   DBNGZC010000012 DBNGZC010000000
VERSION     DBNGZC010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2642710"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="AC16G0_00170"
     CDS             complement(<1..533)
                     /locus_tag="AC16G0_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098063.1"
                     /translation="MAARSPGEGGLVPFAFYRRLTRGQQRAYRQSDEVAAVRLPGAED
                     FRPLVDALAVALENGDRAATQAAADRLLARMTAALRVAPLRAEVQTVRPSRHWGELHG
                     LYTPGSNGRLAQVTVWMRTAQRHQVVKFRTFLRTLLHELGHHLDYEMLGLPDSFHTEG
                     FYKRESSLLRQLLGTDI"
     gene            complement(557..1498)
                     /locus_tag="AC16G0_00175"
     CDS             complement(557..1498)
                     /locus_tag="AC16G0_00175"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018394737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR5098064.1"
                     /translation="MGKMTEMREAWGQTMLELTKTHPNVMVMDGDLSNSTRSDIVAYN
                     APDFYMNMGIAEQNMMGVAAGMASVGLIPWVSSFACFLANRSLDQLRVTVAQPHLNVK
                     LAGHYSGILTGKTGKTHIDVEDIAIMRAMPNMVVVAPADAVECRLAALALTDLQGPAY
                     LRVIRDAVPAIFGEDYQFVLGKVVPLREGKDLTIFGTGAQTGRALEACDLLAAEGISA
                     HLVHVPTLKPMDVGGIVAAAKQTGRVLTTEDHTILGGLGGAVAEVLGQHHPVPMKIHG
                     LQDCFGESGPNDAMLEKYGLTPKHVAAAAKALLQKRR"
BASE COUNT          263 a          559 c          561 g          295 t
ORIGIN      
        1 tcgatatccg tccccaggag ctgtcgcagc aggctcgact cgcgcttgta gaagccctcg
       61 gtatgaaagg aatccgggag gccgagcatc tcgtagtcca ggtggtgacc cagctcgtgc
      121 aagagcgtac ggagaaacgt ccggaacttg accacctggt ggcgctgggc ggtccgcatc
      181 cacaccgtga cctgggcgag ccgtccgttg cttccaggtg tgtacaagcc gtgcagttcg
      241 ccccagtgac gggacggccg caccgtctgc acctcggcgc gcagcggggc gacccggagg
      301 gccgccgtca ttcgcgccag gaggcggtcg gctgccgctt gggttgccgc gcggtcaccg
      361 ttttccagcg ccacggccag ggcatccacc aggggtcgaa agtcctccgc acctggcaag
      421 cgaacagccg cgacctcgtc gctctggcga taggcccgct gctgccctcg ggtcagtcgt
      481 cggtaaaatg caaaaggcac gaggccccct tcccccgggg atcgggccgc catcctgccg
      541 cgacgggaga ggatgttcaa cggcgcttct ggagcagcgc cttggccgcg gcggcgacgt
      601 gcttgggcgt gaggccgtac ttctccagca tggcgtcgtt ggggccggac tccccgaagc
      661 agtcctggag gccgtggatc ttcatgggga cggggtggtg ctgccccagc acctcggcca
      721 cggcgccgcc caggccgccg aggatggtgt ggtcttccgt cgtgagcact cggccggtct
      781 gcttggcggc ggccacgata ccccccacgt ccatgggctt cagcgtcggc acgtggacga
      841 ggtgggcgct gatcccctcg gccgccagca ggtcgcaggc ctccagggcc cgaccggtct
      901 gggcgcccgt cccgaagatg gtgaggtcct tcccctcccg caggggcacc accttgccca
      961 ggacgaactg gtagtcttcg ccgaagatcg ccgggacggc atcccggatg acccggaggt
     1021 aggcgggacc ctgcaggtcg gtcagggcga gggcggccag gcggcactcc accgcgtccg
     1081 ccggcgccac gaccaccatg ttgggcatcg cccgcatgat ggcgatgtcc tcgacgtcga
     1141 tgtgggtctt ccctgtcttg ccggtcagga tcccgctgta atgacccgcc agcttcacgt
     1201 tcaggtgggg ctgggccacg gtcacccgca gttgatccag ggaccgattg gccaggaagc
     1261 aggcgaagct gctgacccag gggatcaggc ccacgctcgc catgccggcg gccaccccca
     1321 tcatgttctg ctcggcgatc cccatgttca tgtaaaagtc cggggcgttg taggccacga
     1381 tgtcggatcg ggtggagttg gacaggtccc catccatgac catcacgttt ggatgggtct
     1441 tggtcaactc cagcatggtc tggccccagg cctcccgcat ctcggtcatt ttgcccatca
     1501 ggctcctccc gtcgtgatac tcagggtcaa aggaccaaag accgattctc tatggtccgc
     1561 attggcactc ggggttgcag gaatcggagg tcccactcag tcattggtca ttaggatttc
     1621 gtcgaacccc ggacagtacg gctcaggcgg gtcgctgggc atccagttcg gccagcgc
//
LOCUS       DBNGZC010000013         2942 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_392627, whole genome shotgun sequence.
ACCESSION   DBNGZC010000013 DBNGZC010000000
VERSION     DBNGZC010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_392627"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="AC16G0_00180"
     CDS             <1..294
                     /locus_tag="AC16G0_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HIR5098065.1"
                     /translation="GADAIAGFRSFHGAKWSEEAQAAYPAAVIAEVRRRPFVAGQSIW
                     LLADYQGPAWRTARFTPLQRPKEQNNQGLLDEYRRPKLAYDRVQALYRAWADE"
     gene            926..1750
                     /locus_tag="AC16G0_00185"
     CDS             926..1750
                     /locus_tag="AC16G0_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HIR5098066.1"
                     /translation="MKWITFSRKMGTNGSEVAKRVASELGYRFYDTKAINHMAQELGF
                     LESVREVDEKVPSIFQRLFSQRPMLYLERLYSVIYELAKQGDAVFLGRGSYILLRDFN
                     CALHVRVTASPEKCIHTLMDQGLNREAAARAITRSDDERAAFVKFAFGVNWEDPARYD
                     LILNMDKLSVNLAVSTVIHVVRSPEMSDASRDAISALETMALTSRAEAALIEATFGQG
                     LVTSLSVSVVGPGKVRVSGNVETEGRRVEAEKILRSVKGVESIENAITVVPFQPGM"
     gene            complement(1799..>2942)
                     /locus_tag="AC16G0_00190"
     CDS             complement(1799..>2942)
                     /locus_tag="AC16G0_00190"
                     /inference="COORDINATES: protein motif:HMM:NF013528.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR5098067.1"
                     /translation="ATSALLVHHNDADGLTSGALLSLALEAEGWTVERLALEKIFPEV
                     LAAIHARRAPVILYADLAGQNAGAIAALAGGDSCTLILDHHRPERIQAPHVCLVNPEL
                     VGLSGDVDCSASALAYLFLRHLMGGEGRHADLGVLGALGDGQAADGRLRGLNAGVLLE
                     AEASGSVRRIGDDVRTAVFSRFADRAGADLAADLTRLGSIGYYRGGPALAAAACRDGF
                     DDRLRAYLRELSKLEAERFAAVEARLRREGLRKVGRIQWFDVGDDFASMGVKSVGTFC
                     ERVAAADWADPGSYVVGMQPLPGEIPGLGHFDWVLRKVSVRVPRLLARAVLSGRSPDL
                     MHLVPQASEAAGGFADGCHRLMAAATIPGDATGTFIQALARATEVG"
BASE COUNT          525 a          984 c          934 g          499 t
ORIGIN      
        1 ggggccgacg ccattgcggg cttccgatca tttcacgggg ccaagtggtc agaggaggcg
       61 caggccgcgt accccgccgc cgtgatcgcc gaggtgcggc ggcggccctt cgtggccggc
      121 cagtccatct ggctgctcgc ggattaccag gggcccgcct ggcgcaccgc ccgtttcacc
      181 cccctgcagc ggcccaagga gcagaacaac cagggactcc tggacgagta tcgccgcccg
      241 aagctggcct acgaccgggt ccaggcgctg taccgagcct gggcggatga gtgaggagca
      301 tatccgtcga ccgggagtgt cgggcccgtc cggcctggga cagcctctcg gacctcgtgc
      361 gcggctcacg cctggctcgc cacattctcc catgtgggca acctggcact ctgggcgaac
      421 cagcgggaat cctgtggggg cgactcgccc caggaccccg gcctccaact tgacaggcat
      481 cccggttcgg cgtagcttcg catcagcttc cccgcatgat tcggtgtccc ggttgggacg
      541 ctgacctttc gcagcccatt cgggcaccgc gctggacacg tttccctgtt ccttctgtgc
      601 caggctctgc gtgcgacaag gaggctttcg tggcttccgt ggcgccgaag atgggactga
      661 cccgctggct atcctcgagc cttctcatcc ccttgcccac cggtgcattc tcccctgaga
      721 gaagccgctg cgtgggtgac tccagccccc ccgttatctc gtccttttcc gcccggaaaa
      781 gcttcgcggt ccgcgccgtc cacaacccag gctgtttcgg atgcctgacc tgtgaccggg
      841 gaaacggcgg cagttcgctc ccgagtctgg ggagattccg taagactctg gagaagaatc
      901 gccgccacct tcaggagggc gtgagatgaa atggattacg ttttccagga agatggggac
      961 caatggatca gaggttgcca agagagtggc aagcgaactc ggctatcggt tctacgatac
     1021 caaggccatt aaccacatgg cccaggaact gggcttcctg gaaagtgtca gggaagtcga
     1081 cgagaaggtg ccttccatct ttcagaggct gttttcccaa aggcccatgc tctacctcga
     1141 acggctctac tccgtcatct acgagctggc caaacaggga gatgcggtct tccttggcag
     1201 ggggagctac attctcctgc gggacttcaa ttgtgccttg catgtccggg tgacagcctc
     1261 cccggagaaa tgcattcata cgttgatgga ccaggggctc aatcgagagg cggcggctcg
     1321 cgcgatcaca cgcagtgatg atgaaagggc agcgttcgtc aagttcgcct tcggggtgaa
     1381 ctgggaagat cccgcgcgct acgacctcat cctcaacatg gataagctga gtgtgaatct
     1441 ggcggtcagc acggtgatcc atgtggtccg ttcccctgaa atgtcggatg cctcccgaga
     1501 cgccatcagc gcgctggaaa cgatggccct caccagcagg gccgaggccg ccctgatcga
     1561 agcgaccttc ggacagggac tcgtcacctc actgtccgtt tccgtggtgg gacccgggaa
     1621 ggtccgggtg agtggaaatg tggagacgga ggggagaaga gtcgaggcag aaaagatcct
     1681 gagatcggtc aagggtgttg aatcaatcga gaatgcgatc accgtggtgc cgttccaacc
     1741 cggcatgtga catctgcagg ggaaggcagg gtgcggatat cagaccgccc gctttcgctt
     1801 acccaacctc ggtggcccgg gccagcgcct ggatgaaggt ccccgtggca tcacctggga
     1861 tcgtggccgc ggccatgagg cggtgacagc cgtccgcgaa accgccagcc gcctcagacg
     1921 cctgcggcac gaggtgcatc aagtccgggg atcggccgct cagcaccgcc cgcgccagga
     1981 gtcttggcac ccgcaccgac accttccgga ggacccagtc gaagtggccg aggcccggga
     2041 tttcgccggg aagcggctgc atgcccacga cgtaggatcc gggatccgcc cagtcggccg
     2101 ccgccacccg ctcgcagaag gtcccgacgg atttgacccc catcgaggcg aagtcgtccc
     2161 cgacgtcgaa ccattgaatc cgtccgacct tccgcaatcc ctcgcgccga agcctcgcct
     2221 ccaccgccgc aaatcgctcc gcttccagct tgctcagctc gcgtaggtac gcccgcaggc
     2281 gatcgtcgaa gccgtcccga catgcggcag cggccagcgc gggtcccccc cggtagtacc
     2341 cgatggagcc gagacgcgtg aggtctgcgg ccaggtccgc cccggctcga tcagcaaagc
     2401 gggagaagac ggctgtccgg acatcgtccc cgattcgccg gacggatccg ctggcctcgg
     2461 cctccagcag gaccccggca ttcaatcccc gcagcctgcc gtctgccgcc tgcccatccc
     2521 caagggcccc cagaacgccg aggtccgcat gccgaccctc tccgcccata agatgccgga
     2581 ggaacaggta cgcgagggcg ctggccgagc aatccacatc cccggacagc ccgaccagct
     2641 ccggattcac caggcagaca tggggcgcct gtatccgctc gggccgatgg tggtccagta
     2701 tgagcgtgca ggagtcgcca ccagccagcg cggcgatcgc gccggcgttt tgccccgcca
     2761 ggtcagcgta caggattacc ggtgcgcgac gcgcgtgaat cgccgccagc acctccggga
     2821 agatcttttc cagggcaagc cgctcgaccg tccagccttc ggcctcgagc gccaggctga
     2881 ggagcgcccc cgacgtcagg ccgtcggcgt cgttgtggtg gacgagcagg gcggatgtcg
     2941 cg
//
LOCUS       DBNGZC010000014         1736 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1468832, whole genome shotgun sequence.
ACCESSION   DBNGZC010000014 DBNGZC010000000
VERSION     DBNGZC010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1468832"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="AC16G0_00195"
     CDS             complement(<1..1112)
                     /locus_tag="AC16G0_00195"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098068.1"
                     /translation="MGAGVKQRLGIRRREFLRVGGVAAAAAALTGVLPRVRRAFAAEE
                     PVRIGILGPLTDFTGRDIQRAAQIAADEINPGGGILGRQIQLFSGDSEGVPEKAIQAF
                     QQLVVRDKVHAVIGGFRSGAVLALVPYVGRLKVPFIITGAASPDIMKPVTENYATYKY
                     LFRAWVNSERQALSLAFVCRDILKAQAGFTRFAIDAENLKWARDYADILKVKLKEYNL
                     ELVYETSHDPATTDFTPIFKAAQAAKAQALLEIISNQAGFVIVKQWRDQRVPMALAGN
                     NNPSYLISSFWKDTDGKCKYELSAYTKAPLSSKSLPFWDKFEKRYGETPFYTGTGAYD
                     AVYLVKVAAEAAKSMDRDALVAALEKVDYEGIIGRIR"
BASE COUNT          299 a          568 c          538 g          331 t
ORIGIN      
        1 cggatgcgcc cgatgatccc ctcatagtcc accttttcga gcgcggccac cagggcgtcc
       61 cgatccatgg acttggccgc ctccgccgcc accttgacga ggtagaccgc atcgtaggcg
      121 ccggtcccgg tgtaaaacgg cgtctcgccg taacgcttct caaatttgtc ccagaagggc
      181 agcgacttgc tcgacagcgg cgccttggtg taggcggaca gctcgtactt gcacttccca
      241 tcggtgtcct tccagaagga ggaaatcaga taggacgggt tgttgttgcc ggccagggcc
      301 atgggcacgc gctggtctcg ccactgcttc acgatcacga atcccgcctg gttggagatg
      361 atctccagca gagcctgggc cttggcggcc tgggccgcct tgaagatcgg cgtgaagtcc
      421 gtcgtggcag gatcgtgcga ggtttcgtac accagctcca gattgtattc cttcagcttg
      481 accttgagga tgtcggcata gtcccgagcc cacttgaggt tctccgcgtc gatcgcaaag
      541 cgggtgaaac ccgcctgggc cttcaggatg tcccggcaca cgaaggccag gctgagcgcc
      601 tgccgctcgg aattgaccca ggcgcggaag aggtacttgt aggtggcgta gttctcggtc
      661 accggcttca tgatgtccgg gctggccgcc ccggtgatga tgaacggcac tttgaggcgc
      721 ccgacgtagg ggaccagggc gagaacggcg ccgctgcgaa accccccgat gaccgcgtgg
      781 accttgtcgc ggaccacgag ctgctggaac gcctggatgg ccttttccgg cacgccctcg
      841 gagtctccgc tgaacagctg gatttgccgg cccaggatgc cgccccccgg gttgatctcg
      901 tcggcggcga tctgcgccgc ccgctggatg tcgcgcccgg tgaaatccgt gagcggtccg
      961 aggatcccaa tccggaccgg ctcctccgcc gcgaaggctc gtcggacgcg gggcaggacc
     1021 cccgtgaggg cggctgcggc ggccgcaact ccacccaccc tgagaaactc tcgtcggcga
     1081 atccctagac gttgcttcac tccggcgccc ataatgtctc ctccttcttg tggcgatggc
     1141 ttcagacggc cgctcctcgc tccaggttcg gttctctggt acccgtcctc cgtcctcggc
     1201 tcaggctgga ccacgagccg ggattccgcg cggccaaaga tgagcgggcg cgatgcagct
     1261 cggcccaaga ttgatactga tgcaatacgg cgccgcggac gcgaagactg gagaggtttc
     1321 ccgcgaggga aatgagacat ggacaaccga ggggcagctc acaactatcg actccccatc
     1381 ctgccgcgac gtgcgtcgca gccatcggat acctcagcgc tcggccgttc gatccgaagg
     1441 gaaaacttgg cacgtgaaac tcccaagttc ctccatgctt gtcaagggaa aatcgcgctg
     1501 atcgcgttga tccgtaccag tcctggcccc tatcgggccc gtctgtttgt gcactcctcc
     1561 ctccgttctg acgagatgtc cacagcatct tcctgacgtg ggttccgggc cagcgaaggc
     1621 aggagcagtc cagcgtcttg cccgggatca gctgccaacc cgcccggccg tgaagaggct
     1681 cttgggcagc atagcgtgaa cacctttcac cgtgttcacg agctgcgtga tccgga
//
LOCUS       DBNGZC010000015         4542 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2055856, whole genome shotgun sequence.
ACCESSION   DBNGZC010000015 DBNGZC010000000
VERSION     DBNGZC010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4542)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4542)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4542
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2055856"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="AC16G0_00200"
     CDS             complement(<1..313)
                     /locus_tag="AC16G0_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098069.1"
                     /translation="MPGTGTAVQSPQVKLERVEVSSYFPYAPPPARVPLVLGFIYNLT
                     NPNEIPVTLEEFKFTTSFEAKPGEYFPLSTPIVYETMQIPGKATNQLRVTVVLDSLIV
                     PG"
     gene            complement(684..1451)
                     /locus_tag="AC16G0_00205"
     CDS             complement(684..1451)
                     /locus_tag="AC16G0_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5098070.1"
                     /translation="MYQEVILALKGVSLEVPPGGIVGLLGANGAGKSTTLKAISGLLK
                     HENGEVTDGSIEFLGQRIDRKSAEEIAGMGIIQVIEGRRVFEHLTVEENLRVGAHLRG
                     NGAGMRRGLDSVYHYFPRLKERRDIQAGYVSGGEQQMTVIGRALMAQPRIMLLDEPSM
                     GLAPMLIKEIFEIITRLNRDQGISILLVEQNVKLALATASYAYVLETGRVVMDGMAET
                     LRENPDIRDFYLGLTDLGARKSVRAVKHYKRRKRWLT"
     gene            complement(1507..2790)
                     /locus_tag="AC16G0_00210"
     CDS             complement(1507..2790)
                     /locus_tag="AC16G0_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098071.1"
                     /translation="MRRRMGMVVCLLVVLAGGVPAQAQTVVKMGTLADMTGPTSDVGK
                     DVAIGMREAVQYVNDTGGINGKPIKMFLYDYGYRVQEAITTYKRFLDFDKVVAVWGWG
                     TGDTEALSPTVNADKMPFLSDSFSSNLTDPKKTPYNFVYGTDYSTSARGALTAWYDEV
                     WLKDPKFKADREKGKKPRFVCFYSWPTPYSSAPIKAIKDQAALLGFEVGPDQDVSLTA
                     LDTKSQVLAAKSFGPHVVWHGNTTMSVATALKDAAALGLGADHLVNIWGYDENLIKLA
                     GASSERVIGTPANAFYGEKVPLMDKVVEYAKKVDPNVPQENRLVRTVQAWVDVLLMRE
                     ALVRADKAGKLFDQSGKELPQAVRGPAMKDAFESIREWTPGLGRAPITITPTDHRPGS
                     LVRVYVIRDRKFTLLASVDLKKRWPEKWEKEWLGW"
     gene            complement(2877..3941)
                     /locus_tag="AC16G0_00215"
     CDS             complement(2877..3941)
                     /locus_tag="AC16G0_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5098072.1"
                     /translation="MYLPSGVYHESYAQDQAWWQTHLIRAKMLGLLLFLLAVFPLGAN
                     LYLVSVANVVGYTILSAMGVQLLIGYCGQLTLGHAAFIAVGAYTTAIAMLHYHLPYPA
                     GLILGAIAAGLWSIVFGLPSARVKGFYLIMTSMAAQFITVDFIITQYVSQIGGRGTFF
                     SIPPGTIRIGPWVIDSDLKVYYLMVVLVILCTAAVANLLRTRVGRAWIAIRDNDIAAE
                     AIGVNVVAYKLLAFFVAGVLGGIAGGFWISSLLFVSPEHFQWSWSLWIVGVILIGGTG
                     SLHGTIFGSLFLSLILEALKFVVIPLSEVYPALQEGFVYVKESAFGLAIVLFLLYEPK
                     GLAYRWQQIKHYFNLWPFSY"
     gene            complement(3994..>4542)
                     /locus_tag="AC16G0_00220"
     CDS             complement(3994..>4542)
                     /locus_tag="AC16G0_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5098073.1"
                     /translation="WGTDTRVFAPPVFSQEPIQLGRVIGGQVYVWSFLAALAMLGVVV
                     AFFKYTRWGLSMQATADDEMASLSLGVSAHRVYALAWAIAFMAAGVGGTLLGNINGLN
                     ISVGSLGLLVLPAVVLGGLNSVPGAIVGGIAIGVLQNLSDGYLSRFTPGGIKEIAPFV
                     FMVVILLFRPYGLWGWTRIERV"
BASE COUNT          853 a         1524 c         1358 g          807 t
ORIGIN      
        1 tgcctggaac gatcaacgag tccaggacca cggtcactcg aagctggttc gtggctttgc
       61 ctggaatctg catcgtctcg tacacgatgg gggtgctcag cgggaaatac tcgcccggct
      121 tcgcctcgaa ggaggttgtg aacttgaatt cctccagcgt gaccgggatc tcgttgggat
      181 ttgtcaggtt gtagatgaat ccgagcacga gaggcacccg cgccgggggt ggtgcgtatg
      241 ggaagtagct ggaaacctct acccgctcca acttcacttg cggggactgc accgccgtgc
      301 ctgtacccgg catgctggca cacccgaccg cgagcagccc gacaagacaa ccaatggccc
      361 cgagcctgat cccacctgtc cgcatgtctc cccccttccc cgtggcgatc gtttgagccg
      421 ggtgaatcga cgatgggtgc gggctggtgg agccttccac cagcccgatc actctccttc
      481 tgatggtccg cccacttcgc gtacggctag taacctcctc cgcccccgcc gccgcctttg
      541 ctctcaacct gatccggcgg gatcggcgcg tacgccatgt tttgggcgca cccagccgcg
      601 gcaagcgccg ccaggatgag cacacccgct gcaagtctct gagttccttt ccgcatggtc
      661 atcccctccc tacaaggccc acactaggtg agccaacgtt tccgccgttt gtaatgcttg
      721 accgcccgga cgctcttgcg agccccgagg tcggtcagcc ccaggtagaa gtcccggata
      781 tccgggtttt cacggagcgt ctcggccatt ccgtccatga ccacccgccc cgtctccaag
      841 acataggcat aggacgctgt cgccagggcg agcttgacgt tctgctccac cagcaagatg
      901 gagatcccct gatcccggtt gagccgggtg atgatctcga agatctcctt gatgagcatg
      961 ggggctagcc ccatggaggg ctcgtccagc agcatgatcc ggggctgagc catcaacgcc
     1021 cgcccgatga cggtcatctg ctgctcgccg ccgctcacgt agcctgcctg gatgtcgcgc
     1081 cgctccttca agcgtggaaa gtagtgatag accgagtcca gaccgcggcg catccccgca
     1141 ccgttccccc gcagatgggc acccacccgg agattctcct ccaccgtcag gtgctcgaac
     1201 acccgccggc cctcgatgac ctggatgatg cccatgccgg cgatctcttc cgcggacttc
     1261 cggtcgattc gctgacccag gaactcgatg ctgccgtcgg tcacctcgcc gttctcgtgc
     1321 ttgaggaggc cggagatggc cttgagtgtc gtgctcttgc ccgccccgtt ggcccccagg
     1381 agacccacga ttcctcctgg gggcacctcc agcgagaccc ccttgagggc cagaatgacc
     1441 tcctggtaca tcacctcgat gttgttgatc ttgagcgcgg tctccggccc cgtcacgacc
     1501 gcccccttac cagcccagcc attctttctc ccacttctcg ggccagcgct tcttcaggtc
     1561 aacggacgcc aggagggtga actttcggtc ccggatgacg tacacccgga ccagggatcc
     1621 cggccggtga tccgtcgggg tgatggtgat gggagcccga cccagcccgg gtgtccattc
     1681 cctgatagat tcgaaggcgt ccttcatcgc cggaccgcgc accgcctgag gcagttcctt
     1741 tccgctttga tcgaagagct ttccagcctt atccgcccgc accagggcct cccgcatcag
     1801 gaggacatcc acccaggcct gcaccgtccg caccaggcgg ttttcctggg gcacgttcgg
     1861 gtcgaccttc ttggcgtact cgaccacctt gtccatcagc gggaccttct cgccgtagaa
     1921 ggcgttcgcg ggcgtgccga tcactcgctc ggagcttgcg cccgccagct tgatcaggtt
     1981 ctcgtcatag ccccagatgt tcacgaggtg atcggcgccc aggcccagcg ccgctgcatc
     2041 cttcagtgcc gtggccaccg acatggtggt gttgccgtgc cagaccacgt gtggcccgaa
     2101 gcttttcgcg gccaagacct ggctcttcgt gtccagggcg gtgagcgaga catcctggtc
     2161 cggaccaacc tcgaagccca ggagcgccgc ctggtccttg atggccttga tgggcgcgct
     2221 ggaatacggg gtcggccagg agtagaaaca cacgaaccgc ggcttcttgc ctttttcgcg
     2281 atcggccttg aacttggggt ccttcagcca gacctcgtcg taccaggccg tgagcgcacc
     2341 gcgcgcgctg gtcgaatagt cggtcccata cacgaagttg tacggcgtct tcttgggatc
     2401 ggtcagattg gaagaaaagg agtcggacag aaacggcatc ttgtcggcgt tcaccgtggg
     2461 cgagagagcc tcggtgtccc cggtccccca gccccagacc gccacgacct tgtcgaagtc
     2521 caggaaccgc ttgtacgtcg tgatggcctc ctgcacccgg tagccgtaat cgtacaggaa
     2581 catcttgatg ggtttgccgt tgatccctcc cgtgtcgttg acgtactgca ccgcctcgcg
     2641 cataccgatg gcgacgtcct tgcccacgtc cgaggtcggc ccggtcatgt ccgccagggt
     2701 tcccatcttc accacggtct gcgcctgcgc cgggacgccg cccgccagca cgaccagcag
     2761 gcagaccacc attcccatcc ttcgtcgcat ctcccccctc ctctcgattg agcggttcga
     2821 ctccctgcgt gattcttccc gcccccggtc cggggccggc aggtggcccg ccaggatcag
     2881 tacgagaacg gccacagatt gaaatagtgc ttgatctgct gccagcgata ggccagcccc
     2941 ttcggctcgt acagcaggaa caggacgatg gcgagcccga aggcggattc ctttacgtac
     3001 acgaatccct cctggagggc cgggtaaacc tcactcaacg ggatgacgac aaacttcagg
     3061 gcctccagga tcaggctgag gaagagggag ccgaagatcg tgccgtgcag gcttcccgtc
     3121 ccgccgatga ggatcacccc cacgatccag agagaccagc tccattggaa atgctccggg
     3181 ctcacaaaca gcaaggagga aatccagaag cccccggcga tcccgcccag gacaccggcc
     3241 acgaagaacg ccagcagttt gtaggcgacg acattcaccc caatggcctc ggcggcgatg
     3301 tcgttatccc ggatggcgat ccacgcccga cccactcggg tccggagcag attggccaca
     3361 gccgccgtgc acagaatgac cagaacgacc atcaggtagt acaccttcag gtccgagtcg
     3421 atcacccatg gcccgatccg gatggtcccg ggcgggatgg agaaaaatgt cccccgccct
     3481 ccgatctggc tcacgtactg cgtgatgatg aaatcgacgg tgatgaattg ggctgccatg
     3541 ctggtcatga tcaggtagaa gcccttcacc cgggccgagg gcagcccgaa cacgatgctc
     3601 cagagtcccg cggcgatggc cccgaggatc agccccgcgg gataagggag gtggtagtgc
     3661 agcatggcga tggccgtggt ataggcccca acggcgatga aggcggcatg ccccagcgtc
     3721 aactgcccgc agtagccgat gaggagctgc actcccatgg cggagaggat ggtgtagccg
     3781 acgacattgg cgacggacac gaggtagaga ttcgcaccca ggggaaagac cgcgagcagg
     3841 aacagaagca gacccagcat cttcgcccgg atcaagtgtg tctgccacca ggcctggtcc
     3901 tgcgcgtagg attcgtgata gacaccggac ggaagataca tgaacacctc gcctcaatgg
     3961 aaaacgcacg tgggacaccc gacatccagc gcgtcaaacg cgctcgatgc gggtccagcc
     4021 ccagaggccg taggggcgaa acagcaggat gacgaccatg aagacgaagg gggcgatctc
     4081 ttttatgcct cccggggtga accggctgag gtagccgtcg gacaggttct gcagaactcc
     4141 gatggcgatg ccgcccacga tggccccggg caccgagttc agcccaccca ggacgactgc
     4201 cggcagcacc aggaggccca gggagccgac cgagatgttc aggccgttga tgttccccag
     4261 gagcgtcccg ccgacgccgg ccgccatgaa ggcgatggcc caggccagcg catagacgcg
     4321 atgggcgctc acaccgagcg agagggaagc catctcgtca tccgcggtgg cttgcatgga
     4381 cagcccccag cgggtgtact taaagaaagc cacgactacc cccagcatgg caagggcggc
     4441 gaggaagctc cagacgtaga cctgcccccc gatgacccga cccagttgga tcggctcctg
     4501 gctgaagacc ggaggcgcga agacccgggt atccgtcccc ca
//
LOCUS       DBNGZC010000016         6983 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1371052, whole genome shotgun sequence.
ACCESSION   DBNGZC010000016 DBNGZC010000000
VERSION     DBNGZC010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6983)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6983)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6983
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1371052"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="AC16G0_00225"
     CDS             complement(<1..407)
                     /locus_tag="AC16G0_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HIR5098074.1"
                     /translation="MKRALLALADGTVFQGTGYGAEGETVGEVVFNTCMSGYQEILTD
                     PSYKGQIVTMTYPLIGNYGVNPEDVESRRPFVEGFVIKEGSPLPSNWRGRRSLEDYLR
                     EYGIVGIQGIDTRALTRHIRDHGAQEGVISTMDL"
     gene            complement(550..1854)
                     /locus_tag="AC16G0_00230"
     CDS             complement(550..1854)
                     /locus_tag="AC16G0_00230"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR5098075.1"
                     /translation="MRILIEGGRVIDPAKDVDGVRDVLIEDGRIADVRGSDEQAKGGR
                     PTPSADRILYARGKVVCPGFVDLHVHLREPGREDMETIATGTRAAARGGFTSVCAMPN
                     TNPVNDNQSVTDFVLDRSRREGAVNVFPVGAISKGQQGEELAEIGELIGAGCVAISDD
                     GKPVMNAELMRRALEYASMFQVPVIQHAEDLHMTGKGVMHEGVVSTELGMRGIPAVSE
                     AIIVARDILLAELTGSHYHVAHISTEEAVRLVREAKAKGLHVTCEAAPHHFALTDEAV
                     TSFSTNTKMSPPLRSARHVAAVKAALRDGTIDAIATDHAPHTIQDKEVEFDYAANGII
                     GLETALGLTLTVLVDGGILSLNQAIARLTWDPARTFHLPKGTLAVGADADVTILDPTR
                     EWVVDVKRFASKSKNSPFDGWKLRGQVLCTIVGGKIVWELEG"
     gene            complement(1913..2851)
                     /locus_tag="AC16G0_00235"
     CDS             complement(1913..2851)
                     /locus_tag="AC16G0_00235"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIR5098076.1"
                     /translation="MAIEWKRKDLLGIQSLSLEEMNLVLDTAVSMKEIASRDIKKVPT
                     LRGKTVINLFYEPSTRTRTSFEIAGKWMSADVINISTSSSSVVKGESLKDTGLTLQAM
                     HPDVVVIRHQAAGAPHLLAGLLKASVINAGDGAHEHPTQALLDLFTIRERRESLQGLK
                     AAIVGDILHSRVARSNIFAMRKAGMQVRVCGPPTLLPRHVETLGVQKYLAVEPAIADV
                     DIIMMLRIQRERMGAGLFPTLREYSKLFGLNPDRLKMAKPDVLIMHPGPINRGVEIAP
                     EVADGPYSLILDQVENGLAIRMALLYLLAGGQAVSN"
     gene            complement(3006..4730)
                     /gene="pckA"
                     /locus_tag="AC16G0_00240"
     CDS             complement(3006..4730)
                     /gene="pckA"
                     /locus_tag="AC16G0_00240"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIR5098077.1"
                     /translation="MTNLLDIRTPAQGEARGLKGDYGLDNHGLTNLNQAYWNLPTEAL
                     YEEATFRREGRITHEGPLVVNTGKHTARAANDKFIVREHGSKDHVWWGQYNRPFAPDK
                     FNELFNRLQGFFQGRDLFVQDCYVGADPEYRLPVRVITEYAWHSLFARNMFLLPKSLE
                     EYRQHVPEFTVICAPSFKGIQQIDGTASDTFIAASFDQRLCIIGNTAYAGEIKKSVFT
                     VLNYLLPLQDVLPMHCSANRGKDGDVAIFFGLSGTGKTTLSADPERGLIGDDEHGWSD
                     QGVFNFEAGCYAKVIQLSPTAEPQIYACTQRFGSILENVVYDPTTRRIDLDDESLTEN
                     TRASYPLEYIDHAVPEKLGGHPKNIILLTCDASGVMPPIAKLTPDQALYQFISGYTSK
                     VAGTEVGLGREPEITFSTCYGAPFMVHHPSVYADLLKRKIQRHGVNCWLVNTGWIGGP
                     YGVGKRISIAYTRGLLKAVLGGELLGVEYATDPIFGYQVPRSCDGVPPRVLNPAEAWP
                     SKEQYMQRYRQLAFRFADNFKKFAAGCPPEVAQAGPAGLTTIHARPISWKAEPAPRQA
                     EPEAVNAD"
     gene            complement(4727..5944)
                     /locus_tag="AC16G0_00245"
     CDS             complement(4727..5944)
                     /locus_tag="AC16G0_00245"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014856044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR5098078.1"
                     /translation="MSISQLARSIAESPTLRLNEEARLLREKGLPVTHLGGGEPKNKA
                     PISAILSSAARLTNADIKYVPTDGIPSLKKAIIRYTEENYERMVQPENVIVSAGAKQS
                     VYNVLFSILNPQDEVIVLAPYWVSYPEMIRMVYGVPVVVTPEDGRYLPRMADIRQAVG
                     PATRAIIVNSPNNPSGLVYPEELVAEIVRFCERKGLYLIMDDIYHKLIFDGKKAASAY
                     KYVSKDLDSSKLIIINGISKLYGMTGFRIGWTIADRKLVEVMNNVQAQTTSCASPITQ
                     AAAEVALTGLQTIVEGLRLTIENNRNVMMQELHSFTGVKVATPDGTFYCFPDFRAYSQ
                     SSVELSNFLLKKALVVTVPGREFGMEGHLRLSYCGSVKDITEGVARMKWALDPNAPND
                     IYIGDRKLIRDWL"
     gene            complement(6012..6566)
                     /gene="pyrR"
                     /locus_tag="AC16G0_00250"
     CDS             complement(6012..6566)
                     /gene="pyrR"
                     /locus_tag="AC16G0_00250"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HIR5098079.1"
                     /translation="MNERREKAQVLDATGIVRAITRIAHEIVERNKGVQDVVLVGLRS
                     RGVGLAHRIASKLEEIEKAKVPVGALDITLYRDDLGRAVTQPVVRRTEISFPINDKKV
                     VLVDDVLYTGRTCRAAMDALMDLGRPCLIQLAVLVDRGHRELPIRADYVGKNVPTSIQ
                     EEVQVLLAEEDGADRVVIFDRIGA"
     gene            complement(6720..>6983)
                     /locus_tag="AC16G0_00255"
     CDS             complement(6720..>6983)
                     /locus_tag="AC16G0_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098080.1"
                     /translation="IHESFVKNLDSMTNDKFPMTNGGGRVHRAVPEHSGGGIRNWSLG
                     FGHSPDYSRTRLRIIGTKDGLSEPGVGQSGEPGGRAAGRQIFA"
BASE COUNT         1136 a         2329 c         2165 g         1353 t
ORIGIN      
        1 aggtccatcg tggagatgac cccctcctgc gccccgtggt cgcggatgtg gcgcgtgagg
       61 gcccgggtgt cgatcccctg gatgccgacg atgccgtact cgcggaggta gtcctccagg
      121 gaccggcgcc cccgccagtt gctgggcagg gggcttccct ccttgatgac gaagccttcc
      181 acgaaggggc gacgggactc cacgtcctcc ggattcaccc cgtagtttcc gatgagcggg
      241 taggtcatgg tcacgatctg acccttgtag gaggggtcgg tcaggatctc ctggtagccg
      301 ctcatgcagg tgttgaacac gacctcgccg acggtttccc cctccgcccc gtaaccggtc
      361 ccctggaaca ccgtgccgtc ggccagggcc agcaacgctc tcttcatctg cgaaacctca
      421 ccctagagcc actggcgtca accgacgcct gcaatgcaaa accggcaact gacaattgaa
      481 aatgggggac aatgtcggtt gttcattgtc cgtttgaaat tgtgcgttgc ggcaagccga
      541 tgcttggtgc tacccctcca gctcccacac aatcttgccc cccacgatgg tgcagagcac
      601 ctggccccgc agcttccagc catcgaaggg ggagttcttg ctcttggagg cgaaccgctt
      661 cacgtccacc acccactccc gggtcgggtc caggatggtc acgtcggcat cggcccccac
      721 cgccagcgtc cccttgggca gatggaaggt gcgggccggg tcccaggtga gacgcgcgat
      781 ggcctggttc aggctcagga tgcctccatc caccagcacc gtcagggtga ggcccaaggc
      841 ggtctccagc ccgatgatgc cgttggcggc gtagtcgaac tccacctcct tgtcctggat
      901 cgtgtggggg gcgtggtccg tggcgatggc gtcgatcgtc ccatcgcgaa gggccgcctt
      961 gacggcggcg acgtggcggg ccgagcgcag gggcgggctc atcttggtgt tggtgctgaa
     1021 gctggtcacg gcctcgtcgg tgagggcgaa atggtggggg gccgcctcgc aggtcacgtg
     1081 taggcccttg gccttggcct cgcgcacgag tcgcactgcc tcctccgtgc tgatgtgcgc
     1141 cacgtgatag tggctgcccg tcagctctgc caggaggatg tcgcgggcca cgatgatcgc
     1201 ctccgagacc gccgggatgc cgcgcatccc cagctccgtg ctcaccacgc cctcatgcat
     1261 gacccccttg ccggtcatgt gcaggtcctc ggcgtgctgg atcaccggga cctggaacat
     1321 gctggcgtat tccagggccc ggcgcatgag ctcggcgttc atcaccggct tcccgtcgtc
     1381 ggagatggcc acgcaccccg ccccgatcag ctcgccgatc tccgccagtt cctccccctg
     1441 ctgccccttg ctgatggccc ccacggggaa gacgttgacc gctccctccc gcctggaccg
     1501 gtcaagaacg aagtccgtca ccgattgatt gtcgttgacc gggttcgtgt tgggcatggc
     1561 gcagaccgag gtgaagccgc cccgggctgc cgccctcgtt ccggtggcga tggtctccat
     1621 gtcctcgcgt cccggctccc gcaggtgcac gtgcaggtcc acgaaacccg gacagaccac
     1681 cttcccgcgg gcatacagga tccgatccgc cgagggggtc ggacggccgc ccttggcctg
     1741 ttcgtccgac cctcggacgt cggcgatgcg tccgtcctcg atgaggacat cccgcacccc
     1801 atccacatcc ttcgccggat cgatgacccg cccgccttcg atcagaattc tcatggttgc
     1861 tccgttcgag gttcgaggtc ggccaaccta gaacattgaa ccgtgaaccc gcctagttac
     1921 tcaccgcctg cccgccggcc aagaggtaca agagggccat gcggatggcc aggccgttct
     1981 ccacctgatc caggatcagg gagtaggggc cgtcggccac ctcgggtgcg atctccacac
     2041 cgcgattgat ggggccgggg tgcatgatca ggacatcggg cttggccatc ttcagccggt
     2101 ccgggttcag gccgaacagc ttggagtact cccgcaaggt gggaaagagc ccggcaccca
     2161 tgcgctcccg ctggatgcgc agcatcatga tgatgtccac gtcggcgatg gcgggctcga
     2221 cggcgagata tttctgcacg cccagcgtct ccacgtgccg gggcaagagg gtgggcggcc
     2281 cgcagacccg aacctgcatg cccgccttgc gcatggcgaa gatgttggag cgcgccaccc
     2341 ggctgtgcag gatgtccccc acgatcgccg ccttcaatcc ctggagcgat tcccgccgct
     2401 cgcggatggt gaacaggtcc agcagggcct gggtgggatg ctcgtgcgcc ccatcgcccg
     2461 cattgatgac cgacgccttg aggagaccgg ccaggagatg gggcgctccg gccgcctggt
     2521 ggcggatcac caccacgtcc ggatgcatcg cctgcagggt caggcccgta tccttcaggc
     2581 tctccccctt caccacgctg ctgctggatg tggagatgtt gatgacgtcg gcgctcatcc
     2641 acttgcccgc gatctcgaag gaggtccgcg tgcgcgtgct cggctcgtaa aacagattga
     2701 tgacggtctt tccgcgcagc gtgggcacct tcttgatgtc gcgggaggcg atctccttca
     2761 tggagaccgc cgtgtccagg acgaggttca tctcctccag cgagaggctc tggatcccca
     2821 acaggtcctt ccgcttccat tctatcgcca tcgcccacct ccggctcgga ttccccgatc
     2881 cgtccaaagc acccctggtc tcgtgctgac acccccggtc tgcgcccgtc cgccaagcct
     2941 atctcggcgg gtacatccat ccggccgatc cctctttgcc cgaggcggcc gatccgtcgc
     3001 tgcagctaat ccgcgttcac ggcctccggc tcggcctgcc ggggtgccgg ctctgccttc
     3061 caggagatcg ggcgggcatg aatcgttgtc aggcccgcgg ggcccgcctg ggcaacttcg
     3121 ggagggcagc ccgccgcgaa cttcttgaaa ttgtccgcga accggaatgc cagctggcga
     3181 taccgctgca tgtactgctc tttgctcggc caggcctctg ccgggttgag gacgcgggga
     3241 ggaacaccgt cacagcttcg agggacctga tacccgaaga tcgggtctgt ggcgtattcc
     3301 acgccgagca gctccccgcc caggaccgcc ttcagcaggc cccgggtgta ggcgatgctg
     3361 attcgcttgc caacgccgta cggcccgccg atccagccgg tgttcacgag ccagcagttg
     3421 accccgtgcc gctggatctt ccgcttcaac aggtcggcgt acacggacgg gtggtgcacc
     3481 atgaacggcg cgccgtagca cgtgctgaag gtgatctccg gctccctccc cagacccacc
     3541 tcggtgccgg cgaccttcga ggtgtagccg gagatgaact ggtagagcgc ctggtccggc
     3601 gtcagcttcg ctattggcgg catcacgccg gaggcgtcgc aggtcaacag gatgatgttc
     3661 ttggggtggc cgcccaactt ctccgggacg gcgtggtcga tgtactccag cgggtaggag
     3721 gcgcgggtat tctcggtaag ggactcgtcg tccagatcga tgcgccgggt cgtcggatcg
     3781 tacacgacgt tctccaggat cgagccgaac cgctgcgtgc aggcgtagat ctgcggctcc
     3841 gccgtcggcg acaactggat caccttggcg tagcagccgg cctcgaagtt gaacacgccc
     3901 tgatcgctcc acccgtgctc gtcgtcaccg atcagccctc gctccggatc ggccgacagg
     3961 gtggtcttgc ccgtcccgga gagaccgaag aagatggcca cgtcgccatc cttgccacga
     4021 ttggccgagc agtgcatggg aaggacgtcc tgcaagggca gcaggtagtt cagcaccgtg
     4081 aagacggact tcttgatctc gccggcgtac gccgtgtttc cgatgatgca cagccgctgg
     4141 tcgaagctgg cggcgatgaa ggtgtccgag gcggtcccgt cgatctgctg gatgcctttg
     4201 aaggaggggg cgcagatcac cgtgaactcg gggacgtgct gccggtactc ttccaggctc
     4261 ttgggcagga gaaacatgtt ccgggcgaag aggctgtgcc aggcatactc cgtgatgacg
     4321 cgcaccggca gccggtactc gggatccgcg ccgacataac agtcctgcac gaacaggtct
     4381 cggccctgga agaagccctg cagccggttg aacagctcgt tgaacttgtc cggagcgaag
     4441 ggccgattgt actgccccca ccagacgtgg tctttgctgc catgctctct cacgatgaac
     4501 ttgtcgttcg ccgctcgggc cgtgtgcttc ccggtgttca ccaccagcgg cccctcgtgg
     4561 gtgatccgac cctcgcgacg gaaggtcgcc tcctcgtaca gcgcctccgt cggcaagttc
     4621 cagtacgcct gattcaggtt ggtcaacccg tgattgtcca gcccgtaatc gcccttgagc
     4681 ccgcgtgcct cgccttgcgc cggtgtcctg atgtccagaa gatttgtcat agccagtccc
     4741 ttatcagttt gcggtcgcca atgtagatgt cgttcggcgc gttggggtcc agggcccact
     4801 tcatgcgggc gactccttcg gtgatatcct tgaccgatcc gcagtagctg agccgcagat
     4861 ggccctccat gccgaattcc cgccccggca ccgtgaccac cagggccttc ttcagcagga
     4921 agttggaaag ctccaccgag ctctggctgt acgcccgaaa atcggggaag cagtagaagg
     4981 ttccatccgg cgtggcgacc ttgacgccgg tgaaggagtg cagctcttgc atcatcacgt
     5041 tgcgattgtt ctcgatcgtc agccgcaagc cttccacgat ggtctggagc cccgtcagcg
     5101 ccacctccgc cgcagcctgg gtgatcgggg atgcgcagga ggtggtctgg gcctggacgt
     5161 tgttcatgac ctcgaccagc ttccggtcgg cgatggtcca gccgattcga aagccggtca
     5221 tgccgtacag cttcgagatg ccgttgatga tgatcagctt cgaggagtcc aggtccttgg
     5281 acacgtactt gtaggccgag gcggccttct tcccgtcgaa gatcagcttg tggtagatat
     5341 cgtccatgat caggtagagg cccttccgct cgcagaaccg cacgatctcc gccaccagct
     5401 cctccgggta cacgagaccg gatggattgt ttgggctgtt cacgatgatg gcccgggtgg
     5461 cgggacccac agcctgccgg atgtccgcca tccggggcag gtagcggcca tcctcgggcg
     5521 tgaccaccac cggcaccccg tagaccatcc ggatcatctc tggatagctc acccagtacg
     5581 gggccagcac gatgacctcg tcctggggat tcaggatgct gaagagcacg ttgtacaccg
     5641 actgcttggc cccggccgag acgatcacgt tctccggctg caccatgcgc tcgtagttct
     5701 cctcggtgta gcggatgatc gccttcttga gcgaggggat cccgtccgtc gggacgtact
     5761 tgatgtctgc attggtgagc ctggccgccg agctcaggat ggccgagatg ggtgccttgt
     5821 tcttgggttc cccgcctccg aggtgggtca cgggcaaccc cttctcccgt aagagccggg
     5881 cttcctcgtt gagccgcagg gttggcgact cggcaatcga cctggcaagc tgactgatgc
     5941 tcatgtatgc ctctccctcc tgtccccctc ccgcacgctg gcaaccgcgg tcaacccgtc
     6001 gacatcgccc ctcaggcgcc gatgcggtcg aagatcacga cgcggtccgc gccgtcctct
     6061 tcggccagca agacctggac ctcctcctgg atggaggtcg gcacgttctt ccccacgtag
     6121 tcggcccgga tcggcagttc ccgatgcccg cggtccacca gcaccgcgag ctggatcagg
     6181 cacggccgcc ccagatccat gagggcatcc atggccgccc ggcaggtgcg gccggtgtac
     6241 agcacgtcat ccaccaggac caccttcttg tcgttgatgg ggaacgagat ctcggttcgc
     6301 cggacgactg gctgggtcac ggcccttccc aggtcatcgc gatacagcgt gatgtccagc
     6361 gcccccacgg ggaccttggc cttctcgatc tcctccagct tgctggcgat tcggtgcgcc
     6421 aggcccaccc cccggctgcg caggccaacc aggacgacgt cctgcacacc cttgttccgc
     6481 tccacgatct cgtgcgcgat gcgggtgatg gcgcggacga ttcccgtcgc atccaggacc
     6541 tgggccttct cacgtcgctc gttcataccg cctccgggca caaaaaaacc tcctcgcccc
     6601 tgagggcgag gaggttgttg gaatccagca gtctgagcgg cagcaccttc ggctccttta
     6661 cggacttcac ggagtccgct taaaaggtgc catgtttgta tcagaggcgg cttcagccgt
     6721 caagcgaaaa tttgccgccc ggcagcccgg cctcccggtt cgccggattg tccgacgccc
     6781 ggttcggaga ggccgtcctt agtcccgatt attcgcaaac gagttcgcga ataatcgggg
     6841 gaatgaccaa atcccaatga ccaattgcgg attccccctc cggaatgttc cgggacggcg
     6901 cggtgtacgc gcccaccacc attggtcatt ggaaatttgt cattggtcat tgaatccaga
     6961 tttttcacga aactttcgtg aat
//
LOCUS       DBNGZC010000017         2407 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1175449, whole genome shotgun sequence.
ACCESSION   DBNGZC010000017 DBNGZC010000000
VERSION     DBNGZC010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1175449"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            577..1260
                     /locus_tag="AC16G0_00260"
     CDS             577..1260
                     /locus_tag="AC16G0_00260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020881568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR5098081.1"
                     /translation="MAHSAQIRMDRRAFLHLTAATAVVTAAGRTGLGNAAQDLQSIPL
                     PPPQLDGGRPLMQVVKDRRSARAFSQKPLPSQVLGNLLWVAFGVSRQDSGGRTAPSAS
                     NSQEMEIYVATADGLFLYAAKAHRLQPILGEDIRALTGRQGFVKDAPVNLIYVSELAK
                     MKAAAEDREFYAGAHAGFIGQNVYLFCASEGLATVVRASIDRQALARRMGLRPEQRIT
                     LSQSVGYPA"
     gene            complement(1754..>2407)
                     /locus_tag="AC16G0_00265"
     CDS             complement(1754..>2407)
                     /locus_tag="AC16G0_00265"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HIR5098082.1"
                     /translation="GSPLRAMAHRGVAEVSLAEDLARVQAVRKAIGPEVRLMIDANGA
                     WDVGTAVKMGRAFEPSDIYWYEEPVCPDDLLGSAEVASKVTIPVAGYETCSYGRVSFR
                     DYILARALHFVQPDVAWAGGLTESLKIAHMAQAWNLPFAPHIHGSAVAVAAAAHMLGA
                     VPNASMAEMVFPAHPLMAELVKEPLVVDSTGHIELTDRPGLGIELDPGVVTKYRVDG"
BASE COUNT          414 a          787 c          772 g          434 t
ORIGIN      
        1 aagaatcaag cacttagact cgacgagtgg ttcgatgtgt tgcgggctac ttggtagcag
       61 acgtggcaac agaagtcaag gtcatcaggc gaggatgtac cgtcgaactt tcgacccgac
      121 gaccgaggcc tgctgactgg caagcagatg cgatccggcg acctcgggaa acggtcagct
      181 cggccgaggc agcgctcaca cctgtctacc caggatggca tcgcctcgcc aggctggccg
      241 cttctcccgt ggtcctgcat agcccatcgg acaaccggcg gcctcgtgtt gacgccgcct
      301 gcaagggagg ggtaggcttt gtccgagcat cttgccatcc atctacacca tcgcgggagt
      361 gctcaaccat cctctagctc ccgcgaaaaa cggggcttgt tggcctttcc agttgccacc
      421 cagaggtcag caaccgatct ggatgcgcat gatcttgagc aaaccgtcct ggaatcgtgg
      481 gatcgtttgg ggccactggc atggcacaga ctcggtcatt gtagttgcgg ggcgctggcg
      541 gcgttgcggc cacctcgcgc ccgaaggagg gacggcatgg cgcactcggc acagatccgc
      601 atggatcgac gcgcattcct ccacctcacc gcggccaccg cggtggtaac cgcggcgggc
      661 cggacgggcc tcggcaacgc cgcgcaggac cttcagtcca tcccgctccc gcctccgcaa
      721 ctcgacggcg ggcgaccctt gatgcaggtg gtgaaggacc gccggtcggc ccgggccttc
      781 agccagaagc cgctgcccag ccaggtgttg ggcaacctcc tgtgggtggc gtttggcgtg
      841 agtcgccagg actccggcgg gaggactgcg ccctcggcga gtaactccca ggagatggag
      901 atctacgtcg ccacggctga cggcctcttt ctctacgcgg cgaaagccca ccggctgcag
      961 ccgatcctgg gggaggacat ccgcgccctg acggggcgac agggattcgt gaaggacgcc
     1021 cccgtgaacc tgatctatgt gtccgaactg gccaagatga aggccgctgc ggaagatcgg
     1081 gagttttacg ccggggccca cgccgggttc attggccaaa acgtctatct gttttgcgcg
     1141 tcggaaggcc tggcgactgt ggttcgcgcg tccattgatc gccaggccct ggctcggagg
     1201 atggggcttc gtcccgaaca gaggatcacc ctctcccagt ccgtcgggta tccggcgtag
     1261 ggtcgatccg ggaaccaagg ccaggaccgg gacgacagat accgggcggc tcatcgatcg
     1321 gggggtaccg ccccgccctc atcggcgccg taggcgtgtc cgggggccgg cattctgctg
     1381 atgctgatcc ccctgcctcc cgcaatcatc gcccgaccca gctttctggc atcagggatg
     1441 tttgcgcaca ggcaaccgcc tccacccagg tcgggctgcg ggggaggccc ggtcatcggc
     1501 acctggcctc tatctcagtt tgtgaaatct cggtgggacg gctggttccg tggctctggg
     1561 atttctctgg agccgcttgg gtggtccgtg tcgcatttgt cctctagatg caggggacgg
     1621 gggacgatgg ctcggattac ctccacgcca tggaccgatt agcgccccag catctggtgg
     1681 cgatatccgg atgagcatca acgccaccct tccctaccgt ttttgactgg acccttggag
     1741 gagatggctc ctcttatcca tccacccggt acttcgtaac cacgccagga tccagctcga
     1801 tgcccagacc cggacggtcg gtcagctcga tgtgcccggt agagtccacc accagcggtt
     1861 ccttgaccaa ctcggccatc agggggtggg ccgggaagac catctcggcc atgctggcat
     1921 tgggcacggc ccccagcata tgcgctgcgg ccgccacggc cacggcagac ccatggatgt
     1981 gcggggcgaa gggcaggttc caggcctggg ccatgtgggc aattttcagg gactccgtca
     2041 gccctcccgc ccaggcaaca tcgggctgga cgaagtggag cgcgcgggcc aggatgtagt
     2101 cgcgaaaact cacccggccg tagctgcacg tttcgtatcc ggccaccggg atggtcacct
     2161 tgctcgccac ctcggcgctc ccaaggaggt catccgggca gaccggctct tcataccagt
     2221 aaatatctga gggctcgaag gcccgtccca tcttcacggc cgtccccaca tcccacgcgc
     2281 cattggcatc gatcatgagg cggacctctg ggccgatcgc ctttcggaca gcctggacgc
     2341 gggcgagatc ctcggcgagt gagacctcgg ccacaccccg atgggccatc gctcggaggg
     2401 ggctccc
//
LOCUS       DBNGZC010000018         5262 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2056010, whole genome shotgun sequence.
ACCESSION   DBNGZC010000018 DBNGZC010000000
VERSION     DBNGZC010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5262)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5262)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5262
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2056010"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..1010)
                     /locus_tag="AC16G0_00270"
     CDS             complement(195..1010)
                     /locus_tag="AC16G0_00270"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR5098083.1"
                     /translation="MASGRSRTHQSLDRGLRIIEATAAIGGAATLSEIARKTGLPRST
                     THHLLRALVDFGYLVQDGETRPYTLAPKLFRLTGCTWTNEQLAEISMPFLDELSRRTG
                     EGTSLAILRDGVVTVVAKRESEGPVRVVQEVGARRPIYCTAVGKALAAWLPERELDGM
                     IARTVFEPKTARTIRSPAAFRRDLARIRATGFAIDNQEHIQGIRCIATPVRDHSGEVR
                     ASLCVLGPTSRLPQRRLMEIRRPLAAVAADLSTRLGYGSAEGDNPIAEDRTCR"
     gene            complement(1077..1307)
                     /locus_tag="AC16G0_00275"
     CDS             complement(1077..1307)
                     /locus_tag="AC16G0_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098084.1"
                     /translation="MVVEPKTQDLPAILRRETDGLGPECTFDCVGNATLLEQAIGLVR
                     RLFPGIVLSAPSRPKTHRMHANPFVPGGGVAG"
     gene            1534..2352
                     /locus_tag="AC16G0_00280"
     CDS             1534..2352
                     /locus_tag="AC16G0_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR5098085.1"
                     /translation="MGSVAKVFRILETVVAHQSRGLAYSEVVARTHLPKATAHRLLKS
                     LVQAGYLRFDAEAGRYYGDLKLSSLGSAVTSHFDLKEYVRPHLMRLQAETKHTSNLGV
                     LSGEAGVYLDKLESSEAYGIKLYSAIGKRFPLHCTAMGKVLLAGMTPAARRKILARKL
                     ETFTPSTITDPAALERELNTVRRCGYALDREEITRGIMCVAAPIQNSEGETLAAISLA
                     FPAYIREERGLRDEIRAVTRCAVEVTRLLAGKSGGNGALRRRGGHLAPATKKRG"
     gene            2356..3147
                     /locus_tag="AC16G0_00285"
     CDS             2356..3147
                     /locus_tag="AC16G0_00285"
                     /inference="COORDINATES: protein motif:HMM:NF016826.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HIR5098086.1"
                     /translation="MGNAWLFRGTHRVQGRKRIIHQHNCGLEFLRYGRIVLGSGGGKV
                     DLESQDEELGFVCLSGTGAIRVDGQSYSLGKYDALYLPRDSRCTVSSAGAFDLAEIGA
                     PSSQKYPVQFVRFEDVLKNPKLVRKAGFEPYARTLHTLIGEDNVQAARLLAGVTFSQD
                     GNWTSWPPHDHHKEKEEIYLYIDMPEPNFSLHLNYTDGKDMEMVVPVWEGDAVAIKKG
                     YHYNVATPGTATGFLWMMAAVREEKDRVFTQVTVQPEFDGRFKLF"
     gene            3216..3584
                     /locus_tag="AC16G0_00290"
     CDS             3216..3584
                     /locus_tag="AC16G0_00290"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5098087.1"
                     /translation="MSEQQPKYYYHIADVPLVPLTDKVGTRFIAGQKILCSFIEQPPG
                     ATFPIHSHPAEQILVILEGSEEHICGGEKFTMKAGDVCVHPSNVPHGGHTPGGFKGID
                     IFAPPREDYLELMRKHGLLK"
     gene            3672..4691
                     /locus_tag="AC16G0_00295"
     CDS             3672..4691
                     /locus_tag="AC16G0_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR5098088.1"
                     /translation="MVTRRQVLSGVIGVTAAAGLRGGLLRPSPAKAQALRMKFAHFAD
                     ENHPGHLAAKMFAEAVDKRTNGQIKIDIFPNNMLGSPPEQAEQVKLGTTDMGLPTQGQ
                     FDKWIKAMGVVMLPFAYDDYDHVHRTVDGPAFQWFSQLAEKEGFILLSNWEYGFRNLT
                     NNKRAINAPEDVKGLKIRVPPEMQIGAAFEALGAQTTVIAFPELYMALAQGVADGEDN
                     PVSVIYFMKFYEVQKHLALTRHIYNNMIHTISAKTWAKLTPEQKTIFQEESKRAGAFM
                     RQQIVSQEGDLVAKMQQGGTQVTRPNLALFRAAMKPAYDKIFKYVGEENGKKYMEFVE
                     TARKK"
     gene            4802..>5262
                     /locus_tag="AC16G0_00300"
     CDS             4802..>5262
                     /locus_tag="AC16G0_00300"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR5098089.1"
                     /translation="MVTAAIFGLLVLLMFLGIPIAVSMGLTAVLSFLALGTPNLLMMV
                     PQRMYAGTTSFTLLAIPFFILAGNLMNTGGITTRLVHFAQCLVGHIRGGLGHVTVVTN
                     MIMAGMSGAAVADAVGTGNILIPAMERAGYPRVFSAALVGAASTIGPIIPP"
BASE COUNT         1038 a         1602 c         1693 g          929 t
ORIGIN      
        1 ttcggggcct gagcctgcgc cgtggtgcac acgacgacga atgacagagc cagcagcgat
       61 agcagcataa taactctggc ggcggtcttc atgatgcgtc tccttttcct ggagaatcat
      121 gttgccagca gtctggagat gcgtgtggtc tctcgcctcc tttccattga gcatccctcg
      181 aaagtgtcgc ggcgctatcg gcaggtacga tcttcggcga tcgggttgtc gccttccgcc
      241 gagccatagc ccaatcgcgt cgagagatcg gccgccacgg cggcaagcgg ccttcgaatc
      301 tccatcagcc gccgctgcgg caagcggctg gtcgggccca ggacgcagag cgaggcgcgg
      361 acttctccgg aatggtcacg caccggcgtg gcaatgcagc ggataccctg gatgtgttct
      421 tggttgtcga tggcaaagcc ggtcgctcgg atgcgggcca agtcccgacg gaaggcggcg
      481 ggactgcgga tggtccgtgc cgttttcggc tcgaagaccg tgcgcgcgat catgccgtcg
      541 agttcccgtt caggcagcca cgcggccagc gctttgccga cggccgtgca gtagatcggc
      601 cgcctggcac cgacctcctg gacgacccgc acgggaccct cggattcgcg cttcgccaca
      661 accgtcacga cgccgtcgcg caggatcgca aggctggtgc cctctcccgt gcgacgactt
      721 agctcgtcca gaaacggcat ggagatttcg gcgagctgct cattggtcca ggtacacccg
      781 gtgagtcgaa agagcttggg cgccaacgtg taggggcgcg tctcaccgtc ctggacgaga
      841 tagccgaagt cgacgagtgc ccggaggaga tggtgggttg tgctccgcgg gaggcctgtc
      901 ttgcgtgcga tctcggaaag ggtcgcggct cccccaatgg cggctgttgc ttcgatgatc
      961 cggagcccgc gatccaggga ttgatgtgtc cgggatcggc cggaggccat ggggcaagtc
     1021 tcctgtttca tatactgaaa caattgccgt tacgatatct cagactgctg gacatgtcaa
     1081 ccggcaactc ctccgcctgg gacaaaggga ttggcgtgca tgcggtgggt cttcgggcgg
     1141 ctgggggcgc tcagaacgat ccctgggaag aggcgccgaa ccagcccgat cgcctgctcc
     1201 agcagcgtgg catttccgac gcagtcaaag gtgcattccg gcccgagacc gtccgtctct
     1261 cgcctgagga tggcgggcag gtcttgggtc ttgggttcca ccaccatggc cccttctcct
     1321 tggcgacggc cgacgtccgc ggcgtcggag ttggtcggca attcaccatg cgcgaggcgc
     1381 gacggaaccg ggagggaacc acaaaaccac ttgacaattg cccagggtca ggggtaatag
     1441 atttccatat tggaaactaa gttccatata tggaactgca acgactcccg gagggcgact
     1501 ttccgctcat aaaggtatat tggagtcagc gccatgggca gcgtggcgaa ggtgtttcgc
     1561 atccttgaga ccgtggttgc ccatcagtcc cgaggcctgg cctactccga ggtggtggcg
     1621 agaacccatc tgcccaaggc caccgcccac cgcctattga agtccctggt tcaggcgggc
     1681 tatcttcggt tcgacgctga ggcggggagg tactacgggg atctcaagct ctcttccctc
     1741 ggctctgccg tcacctccca ctttgatctg aaagaatatg tccgtccaca tctcatgagg
     1801 ctgcaggccg aaaccaagca tacctccaac ctgggggtcc tgagcgggga agccggggtg
     1861 tatctggata agctggaatc gtccgaggcc tacggcatca agctttactc cgccatcgga
     1921 aagcgcttcc cactgcactg tacggccatg ggaaaggtcc tgctggctgg aatgacgccg
     1981 gccgcgcgtc gcaagatcct ggcgagaaag cttgagacct tcacgcccag caccatcacc
     2041 gaccctgccg ccctggagag agagctgaac accgtccggc gatgcggcta cgcgctggac
     2101 cgggaggaga tcacgcgggg gatcatgtgc gtcgcggccc cgatccagaa cagcgagggc
     2161 gagacgcttg ccgccatcag cctcgccttc cccgcataca ttcgtgagga gcggggactc
     2221 cgggacgaga tccgggcggt gacgcgctgc gcggtggagg tgactcggct gcttgcaggg
     2281 aaatcgggcg ggaacggcgc gctgcgccga cgtggcggcc acctcgcccc agcgaccaag
     2341 aaacgggggt aactcatggg aaatgcctgg ttgtttcgag ggacgcatcg ggtacaggga
     2401 cggaagcgca tcattcatca gcacaactgc ggtctggagt ttctccgata cggccgcatc
     2461 gtcttgggtt cagggggcgg caaggtggac ctcgaatcgc aggacgagga gctgggattt
     2521 gtctgcctca gcggaacagg ggccatacga gtcgacgggc agagctattc gctcggcaag
     2581 tacgacgccc tctatctgcc gcgggacagc cgctgcaccg tatcgtcggc gggcgcgttc
     2641 gacctggcgg agatcggggc gccatccagc cagaagtatc ccgtgcagtt cgtccggttc
     2701 gaggatgtgc tcaagaatcc gaagcttgtg aggaaggccg gttttgagcc atatgcccgc
     2761 acgcttcaca cgctgatcgg ggaagacaac gtccaggcgg cccggctgct ggcgggggtg
     2821 accttcagcc aggacggcaa ctggacaagc tggccgcctc acgaccacca caaggaaaag
     2881 gaggagatct acctgtacat cgacatgccg gagccgaatt tctccctcca tctcaactac
     2941 accgacggga aggacatgga gatggtggta cccgtctggg agggagacgc cgtcgccatc
     3001 aagaaaggtt accactacaa cgtggccacc cccgggacgg ccaccgggtt tctctggatg
     3061 atggcggctg tccgcgagga gaaggaccgc gtcttcacgc aggtgacggt gcagccggaa
     3121 ttcgacgggc ggttcaagct gttttaacca tccctccttt ggccctctcc tccaatcgtg
     3181 gagaggacaa ggaggaggca caaaggagga aaaccatgtc ggagcagcag cccaagtact
     3241 actaccacat cgccgacgtt ccgctggttc cactcacgga caaggtggga acccggttca
     3301 tcgccgggca gaaaatcctg tgcagcttca tcgagcagcc tccgggcgcc acctttccca
     3361 tccacagcca cccggctgag cagatcctcg tcatcctcga aggctcggag gagcacatct
     3421 gcggcggcga gaaattcacg atgaaggcgg gcgacgtgtg cgtgcatcct tcgaacgtcc
     3481 cccatggcgg acacacgccg ggcgggttca agggcatcga catattcgcc ccgccgcggg
     3541 aggactatct ggaactcatg cggaagcacg gcctgttgaa gtgagtggcg cgccgtcgcc
     3601 ggagacgtgt cgggaaacgt ggcgatggga tcggaaggca acgaaggccc aatcacagag
     3661 gaggtcgcgt catggtgact cgacgtcaag tactgtctgg ggtgatcggc gtgacggccg
     3721 cggcggggct gcggggcggt ctcctgcgcc cgtcgccggc caaggcccag gccctgcgga
     3781 tgaagttcgc gcacttcgca gacgagaacc atccggggca tctagccgcc aagatgttcg
     3841 ccgaggccgt ggacaagcgg accaacggcc agatcaagat cgacatcttc ccgaacaaca
     3901 tgctggggtc gcctcccgag caggccgagc aggtgaagct gggcaccacg gacatggggc
     3961 tgcccaccca gggccagttc gacaaatgga tcaaggccat gggagtggtg atgctcccct
     4021 ttgcctacga tgactacgat cacgttcacc ggacggtgga cggtccggcc ttccagtggt
     4081 tcagccagct agccgagaaa gagggcttca tcctcctctc gaactgggaa tacggcttcc
     4141 ggaacctcac caacaacaag cgggcaatca acgccccgga ggacgtgaag gggctgaaga
     4201 tccgggtccc gcccgagatg cagatcgggg cagccttcga ggccctggga gctcagacca
     4261 cggtcatcgc ctttcccgag ctgtacatgg ccttggcgca gggggtggcg gatggggagg
     4321 acaatcccgt ctcggtcatt tacttcatga agttctacga ggtccagaag cacctggcgc
     4381 taacacggca catctacaac aacatgatcc atacgatcag cgccaagacg tgggccaagc
     4441 tcacgccgga gcagaagacc atcttccagg aagagagcaa gagggccgga gccttcatgc
     4501 gccagcagat cgtctcccag gagggggatc tggtcgccaa gatgcagcag gggggcaccc
     4561 aggtgactcg acccaacctc gccctgttcc gggcggccat gaagccggca tacgacaaga
     4621 tcttcaagta tgtcggcgag gagaacggga agaagtacat ggaattcgtc gaaacggcgc
     4681 ggaagaagta ggcggacagt ctccagtccc ttcatcccat gcatcagggg ccccagcttg
     4741 cctggggccc ctgatggctc aagctccatt ctccaggata aggtagagga gaagcaagct
     4801 gatggtcacc gccgcaatct tcggactgct cgtactgctg atgttcctgg ggatacccat
     4861 agccgtcagc atgggcctga cggccgtcct gagcttcctc gccctgggga cccccaatct
     4921 gctgatgatg gtgccgcagc ggatgtatgc tggcaccacc agcttcacgc tcctggcgat
     4981 ccccttcttt atcctggccg gcaacctgat gaacacgggg gggatcacga cccgtctcgt
     5041 tcactttgcc caatgcctgg tcggccacat ccgcggggga ctcgggcacg tgaccgtggt
     5101 cacgaacatg atcatggcgg ggatgtccgg ggcggccgtg gcggatgcgg tgggcaccgg
     5161 caacatcctc atcccagcca tggaaagggc cgggtacccc cgggtctttt ccgcagccct
     5221 ggtgggtgcg gcctccacca tcgggcccat tattcccccg ag
//
LOCUS       DBNGZC010000019         3024 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_197263, whole genome shotgun sequence.
ACCESSION   DBNGZC010000019 DBNGZC010000000
VERSION     DBNGZC010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_197263"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..548
                     /locus_tag="AC16G0_00305"
     CDS             54..548
                     /locus_tag="AC16G0_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HIR5098090.1"
                     /translation="MAITNRPSVNEQHVNDMRSAIEHRATWFYLLVDEAEKAGHSSEE
                     FARKAITRCGVFHGHTKFTQTEDLQAFAREFGNEVYSKIFEMEVKEASADTFEVHFHY
                     CPLVAAWLKHTRDEKKIETLCDIAMDGDRGILSAFPRFTFELGDTIAKGGKVCEIVIR
                     KTRA"
     gene            676..2769
                     /locus_tag="AC16G0_00310"
     CDS             676..2769
                     /locus_tag="AC16G0_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006512723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5098091.1"
                     /translation="MRKLPGMAWVAIGFVPWIMYWILSGLGLWTAAVVAGLLVSIPPN
                     AYRLYRRQAKALELVALAFFATHFLVTVHLGSPLFRSYDAVLINGALAAMAWGTLLAG
                     APFTYQYAREDWPREYWSNSHFRRANEIITVAWGVIFLGNLALGGLSLAHPSARTWLA
                     AVLPNCGIGVGVAFSVWFPRWYPRRVAARRIAEQTPYNWPAPAFASARPEPANRHDVI
                     IVGAGIGGLTAGALLAHRGLKVLVAEQHSQPGGYCTSWAASVQRGDTRLRYVFDAGVH
                     DVSGLGPRGPVRNLLRQLDLEGKLDWRPMQHEYFLPGHHLKVPPRVEDFLAILEGHFP
                     GERQNIRAFFGEMEAVYRELYADVDRTGGVPCYPDSVDEMVAFPRSHPHAFRWMEMPF
                     GKMLDAHFRDPGLRQFLSSLTGYLSDRPDTLTTGSMAPIFGYYFDGGFYPAGGSQSFA
                     NALTEVIRQHGGQICLRTAVSGLRIQEGRAIGVELTSGEVQHAEAVISNADVRKTFLE
                     LVGREHLPKDFARRVEGLEFSASAFEVFLGVDYEPEIAPITKVSLDGRKVEIAIPSKA
                     DPSLAPPGHASLTLVKLVPQAEAITWDRNAPDYALRKRRVGEDLISTAEKVMPGLREH
                     IVWRQDGTPATYARYAWTTGGAIYGPAADQWRPPNKTPIERLYLAGAGVFPGPGIEAV
                     VISGTLAADAVYRRP"
BASE COUNT          512 a          977 c          974 g          561 t
ORIGIN      
        1 agtctcgtcg tgtgagagtc cgcaacaacg caaaccgcac gagggactgt tcgatggcta
       61 ttacaaaccg gcccagcgtg aatgagcagc acgtcaatga catgcgatcc gccatcgagc
      121 atcgcgccac ctggttctac ctgctcgtgg acgaggcgga gaaggcgggg cattcttcgg
      181 aggagttcgc ccgcaaggcc atcacccggt gtggtgtgtt ccacggccac accaagttca
      241 cgcagaccga ggacctccag gccttcgcca gggagttcgg gaacgaggtg tactccaaga
      301 tcttcgaaat ggaggtcaag gaggcctcgg ccgatacgtt cgaggtgcac tttcactatt
      361 gtccacttgt cgcggcctgg ctcaagcaca cccgagacga gaagaagatc gagacgctgt
      421 gcgacatcgc catggacggc gaccgcggca tcctctccgc gttcccgagg ttcacgttcg
      481 agctgggcga caccatcgcg aagggtggga aggtgtgcga gatcgtgatc agaaagacgc
      541 gagcctgagg ctcgagtctg gcgttcctgg ctacattcca cggacccggt cgggccagcc
      601 ccgcggggac gtgcgggctc tgcagcccat cggggacttg ccggaatccg tcaaccgcgg
      661 taggggcctg aattgatgcg caagcttcct ggaatggctt gggtggccat cggcttcgtg
      721 ccctggatca tgtactggat cctgtctgga ttgggcttgt ggactgcggc ggtcgttgcc
      781 ggactcctgg tttccatccc tcccaacgcc taccgtttgt atcggcgcca ggccaaggca
      841 ttggagttgg tggccctggc gttcttcgcc acccacttcc tggtcacggt ccatctgggt
      901 tcccctttgt tccgctccta cgatgccgtc ctcatcaatg gcgccctggc ggccatggct
      961 tggggaacgc tgttggccgg cgcacccttc acctaccagt atgcccgcga ggattggccg
     1021 cgcgagtact ggagcaactc ccacttccgc cgggcgaatg agatcatcac cgtcgcgtgg
     1081 ggcgtcatct tcctcgggaa cctcgccctc ggagggctga gcctcgccca tccttccgcc
     1141 agaacctggc tggccgccgt gctgccgaac tgcggcatcg gcgttggtgt ggctttctcc
     1201 gtttggtttc cccgatggta cccgcgtcga gtcgcggccc gacggatcgc ggagcaaact
     1261 ccctacaatt ggccggcgcc ggctttcgcc tcagctcgtc cggagccggc gaaccgccac
     1321 gatgtgatca tcgtcggggc ggggattggc gggctgacgg cgggagccct gctcgcccat
     1381 cgtggattga aggtcctggt ggccgagcag cactcccagc ccggcggcta ctgcacctcg
     1441 tgggcagcga gtgtccagcg gggcgacacc cgcctccggt acgtcttcga tgccggagtt
     1501 cacgacgtga gcggactggg cccccgggga ccggtccgga atcttctccg gcagttggac
     1561 ctggagggca aactggactg gcgtccgatg cagcatgagt actttctgcc tggccatcac
     1621 ctcaaggtgc ctccgcgggt cgaggacttc ctcgccatac tggagggaca ttttccaggc
     1681 gaacggcaga acatacgcgc cttcttcggt gagatggagg ccgtctaccg tgagctgtat
     1741 gccgacgtgg atcggacggg tggggtccct tgctaccctg acagcgtgga cgagatggtg
     1801 gcctttccac gcagccaccc ccacgccttt cgctggatgg agatgccctt tggaaagatg
     1861 ttggacgccc atttccgcga ccccgggctg aggcaattcc tgtccagcct caccggctac
     1921 ctcagcgatc gtccagacac gctcaccacg ggctccatgg cgcccatctt cgggtactac
     1981 ttcgacggag gcttctatcc tgcaggcggc tcgcaatcct ttgccaacgc gctgaccgag
     2041 gtcatccgcc agcatggagg ccagatatgc ctccgcacgg cggtgagcgg cctgcgcatc
     2101 caggaaggca gggcgatcgg cgtggagctg acctccggcg aggtccagca cgccgaggcc
     2161 gtgatctcca acgccgacgt gcggaagacc ttcttggagt tggtcgggcg ggagcatctg
     2221 ccaaaagatt tcgcgcggcg tgtcgagggg ctcgagttct ccgcttcggc cttcgaggtg
     2281 ttcctgggcg tggactacga gcccgaaatc gcgccaatca cgaaggtgag cctggacggc
     2341 cggaaggtgg agatcgcaat cccgtcgaag gcggatccaa gtctggcccc gcccggccac
     2401 gccagcctca ccctcgtcaa gctggtcccg caggccgagg cgatcacctg ggaccggaac
     2461 gcacccgact atgcccttcg caagcgacgc gtcggggagg acctgatcag cacagccgag
     2521 aaggtcatgc cgggtttgcg ggagcacatc gtctggcgcc aggatgggac cccagccacc
     2581 tacgccaggt acgcctggac caccggaggg gccatctacg ggccggcggc cgaccagtgg
     2641 cggccgccca acaagacacc catcgagcgg ctgtaccttg ccggcgcggg cgtcttcccc
     2701 ggcccgggga tcgaggcagt ggtcatctcc ggcaccttgg ccgctgacgc cgtgtaccga
     2761 aggccctgac cttgaccgac atctctaacc gccaaggaca tccagcgctg gcgctacgtg
     2821 atcttggcgg ttcaagagtt tctttggcct tggggctatc ccaatatgga ctacgatcgc
     2881 attgccgcgg aatacgcccg gaaccgccag gtgcaccccg gagtgttggc cagcctccta
     2941 tccactggcg gtgttggcag tgactcccgg gtgctcgagg tgggatgcgg gaccgggaac
     3001 tacatcgtcg ccttggcagc gagc
//
LOCUS       DBNGZC010000020         2040 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_295147, whole genome shotgun sequence.
ACCESSION   DBNGZC010000020 DBNGZC010000000
VERSION     DBNGZC010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_295147"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="AC16G0_00315"
     CDS             <1..263
                     /locus_tag="AC16G0_00315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR5098092.1"
                     /translation="TLAAQLGPTRVQPGCLRCDLYREVEDQGAITLVEEWESQADLDL
                     RLRSEDYRSVLAAIELAQEPPVVHFDTVTRRAGLEIVASARG"
     gene            389..646
                     /locus_tag="AC16G0_00320"
     CDS             389..646
                     /locus_tag="AC16G0_00320"
                     /inference="COORDINATES: protein motif:HMM:NF015644.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HIR5098093.1"
                     /translation="MRLRGFLFLVIILAFALLSMVGQILGLHIDWLWFREVQFTSVFL
                     TVLRTQVILAAVTAVAFFLIFYVNVLLAWRLAPRDLLVMAP"
     gene            744..950
                     /locus_tag="AC16G0_00325"
     CDS             744..950
                     /locus_tag="AC16G0_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098094.1"
                     /translation="MLTLTGRNSWRRRACDRTAGLRARLLTGDAEAAQVTRGRFHLLY
                     WEHQGQQIFPLLDAQEPPRRQADR"
     gene            993..>2040
                     /locus_tag="AC16G0_00330"
     CDS             993..>2040
                     /locus_tag="AC16G0_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HIR5098095.1"
                     /translation="MDKKHIASVWYFLAAFLILTALQDYVFAPHVETLSYGEFKTLVE
                     KGKVNDLAIGPKIITGRLKREGLAGLLPQARLEALTKSGTGEEPFMTVKVDDPALVHD
                     LQQANVNFRGEVTSTWLPMLLSWLLPMGIFFLIWRYAMKKMGGGGGLGGLMALGKSKA
                     KVYVQKDTGVTFNDVAGIDEAKAELVEVVEFLKTPERFQRLGGKIPKGVLLVGVPGTG
                     KTLLAKAVAGEAHVPFFSLSGSDFVEMFVGVGAARVRDLFAQAVQHAPSIIFIDELDA
                     LGKARGMNPLGGHDEREQTLNQLLVEMDGFDTNNGVIIMAATNRPEILDPALLRPGRF
                     DRQVLVDRPDLKGRE"
BASE COUNT          335 a          667 c          695 g          343 t
ORIGIN      
        1 ggacgctcgc cgcccagctc gggccgacgc gcgtgcaacc gggctgcctc cggtgtgatc
       61 tctaccgaga ggttgaggac caaggggcca tcaccctggt ggaggagtgg gaatcgcagg
      121 ccgatcttga cctccgctta cgctccgagg attaccgctc cgtcctggcg gcgatcgagc
      181 tggcgcaaga gccgcctgtg gttcacttcg acacggtcac caggcgcgca ggcttggaga
      241 tcgtcgcgtc ggccagaggg tagttccgca tgccgcgtcg gtatgccaag gctgggatcg
      301 gaatcggctc gtgaggcttt cgtccggact cggccgtgcg gaccgtcccc agagcacgca
      361 agtaccccca gaaaaggaga gtcgccccat gaggctgcga ggcttcctgt tcctggtcat
      421 catcctcgcc ttcgccctcc tgagcatggt cggccagatc ctgggcctcc atatcgattg
      481 gctctggttc cgcgaagtgc agttcacgtc ggtcttcctc accgtgctcc gaacgcaggt
      541 catcctggcg gcggtcacgg ccgtcgcctt cttcctgatc ttctacgtca acgtgctcct
      601 ggcctggcgg ctcgctccgc gcgatctcct cgtgatggcc ccttagctcg tggcctgtcg
      661 ccatcaggtg gggcgccgga ccggagaatc cagccggggg cgatagggcg cgcaaggatc
      721 ggacgtaggc accgaggatc accatgctga cgttgactgg tcggaactcg tggcggcgac
      781 gcgcctgcga ccggaccgcg ggcctgcgag cccggctcct gacaggcgat gccgaggccg
      841 cgcaggtcac gcgcggcagg ttccacctcc tgtattggga gcaccagggc caacagatct
      901 tccccctgtt agacgcccag gaaccccctc ggcggcaggc cgatcggtag cacgtcccga
      961 cgggggcgat cgcccctcaa ggagaagctg gcgtggacaa gaagcacatc gcgtccgtct
     1021 ggtactttct cgccgccttc ctgatcctga cggcgctcca ggactacgtc tttgcgccgc
     1081 acgtggaaac cctctcctat ggggagttca agaccctggt ggagaagggg aaggtcaacg
     1141 acctggcgat cggcccgaag atcatcacgg ggcgcctgaa gcgggagggg ctcgcggggc
     1201 tgctccccca ggcgcggctc gaggccctga ccaagtccgg gacgggcgag gagcccttca
     1261 tgacggtcaa ggtggacgac cccgcgctcg tccacgacct gcagcaggcc aacgtgaact
     1321 tccgggggga ggtgacgagc acctggctcc cgatgctgct gagctggctc ctccccatgg
     1381 ggatcttctt cctcatctgg cgctacgcca tgaagaagat gggtgggggc ggcggcctgg
     1441 gcgggctgat ggccctcggg aagagcaagg ccaaggtcta tgtccagaag gacacggggg
     1501 tgaccttcaa tgacgtggcc ggtatcgacg aggccaaggc cgaactggtc gaagttgtcg
     1561 agttcctgaa gacccccgag cgcttccagc gactgggcgg gaagatcccg aaaggggtcc
     1621 tcctggtcgg cgtgcccggc accggcaaga ccctgctggc gaaggcggtg gccggcgagg
     1681 cccacgtccc gttcttcagc ctgagcggct cggacttcgt cgagatgttc gtcggggtcg
     1741 gcgccgcgcg ggtccgggat ctgtttgccc aggcggtgca gcacgcgccc agcatcatct
     1801 tcattgacga actggatgcc ctgggcaagg cccggggcat gaatcccttg ggcgggcacg
     1861 acgagcggga gcagacgctc aaccagctcc tggtggaaat ggacgggttc gacaccaaca
     1921 acggcgtcat catcatggcg gccacgaacc gcccggagat cctcgatccg gccctcctcc
     1981 ggcccggccg gttcgaccgc caggtcctgg tggaccggcc ggacctcaag gggcgcgagg
//
LOCUS       DBNGZC010000021         2211 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_980090, whole genome shotgun sequence.
ACCESSION   DBNGZC010000021 DBNGZC010000000
VERSION     DBNGZC010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_980090"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2162
                     /locus_tag="AC16G0_00335"
     CDS             <1..2162
                     /locus_tag="AC16G0_00335"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5098096.1"
                     /translation="KRAEGKLRLQGAALEAAANAIVITDREGRITWVNPAFSRLTGYT
                     VGEALGQNPRLLKSGVHDPAFYRDLWDTILSGRVWRGELVNRRKDESLYIEEQTITPV
                     PDASGVARYFIAIKQDITQRERALEALRQSEQKLALHVQQTPLAVIEWDTETRVVKWN
                     PGAERIFGYTQTEALGRRAADLIVPPSAREHAARVWRDLLAHRGGERSTDANNTKNGR
                     RIQCEWYNTPLVTATGSVIGVASLVLDVTESKVAEEALVARTGQLETIRAVTFEITHE
                     LDLNVLLGLIHRYAAKLVGASAGVIWLWDDQAQLLIPRAWRGLEDLIGTLRYRLGEGV
                     AGTVAQQRVGLLVNDYPTSPYAQAPVVERTGIRAILAEPLLYRGQLLGVLELLNEPPR
                     EGFTEEDRQLLALFAAQAAIAIENSRLYEAVRQQAAVLEERVRDRTQELAAANQQLKA
                     ASRHKSEFLANMSHELRTPLNSILGFAQVLQEQTKEVLGTKQARFLTNILTSGQHLLQ
                     LINDILDISKVEAGKFILQPEPIPVPATLEDILVIARGLANKNSQVIQADIPADLPAL
                     HADPVRFKQILFNLLSNAVKFTPKGGTITLTARQVDGESEQGRIGETETQPPGDGVPP
                     RGRSPDALICGESAYLEICVQDTGMGIKADDLPRLFQEFVQLQASATKENEGTGLGLA
                     LTKRLVELQGGRIWAESSGEGSGSVFTVLLPLRGSGR"
BASE COUNT          431 a          705 c          718 g          357 t
ORIGIN      
        1 gcaagcgggc ggaggggaag ctccggctgc agggggcggc actggaagct gcggcgaacg
       61 cgattgtcat cactgatcgt gaggggcgca tcacctgggt aaatcctgcc ttcagccgtc
      121 tcaccggcta cacggttggc gaagccctgg ggcagaaccc gcgcctgctc aagtcgggcg
      181 tgcacgaccc ggcgttttac cgggatctct gggacacgat cctctccggc cgggtgtggc
      241 gcggcgaact cgtcaatcgt cgaaaggacg agagcctgta cattgaggaa caaacgatca
      301 ccccggtgcc ggacgcgagt ggggtggccc ggtacttcat cgccatcaaa caagacatca
      361 cccagcgcga acgggcgctg gaggcgcttc ggcaatccga gcagaagctg gccctgcatg
      421 tccaacagac tccgctggcc gtcatcgagt gggacacgga gacccgggtc gtgaagtgga
      481 atcccggcgc cgagcggatt ttcggttaca cgcagacgga ggccttggga cggcgcgccg
      541 ccgacctcat tgtcccgccg agcgccaggg agcatgcggc cagggtgtgg cgagatctgc
      601 tggcgcatcg aggtggcgag cggagcaccg atgcgaacaa cacgaaaaac ggtcgacgga
      661 tccagtgcga gtggtacaac acccccttgg tgaccgccac cggaagcgtg atcggagtcg
      721 cctctctggt cctggacgtg accgagagca aggtggcgga ggaggcattg gtggcgcgta
      781 cgggccagct cgaaaccatc cgggcggtca ccttcgagat cacgcatgaa ctggacttga
      841 acgttctcct tgggctgatc caccggtacg cagcgaagct cgtgggtgcc tccgcgggag
      901 tcatctggct gtgggacgat caggcgcaac tcctcatccc tcgggcgtgg cgtggcctgg
      961 aggacctgat aggcacgctc cgttaccggc tgggcgaggg ggtggcgggg acggtggccc
     1021 agcagcgtgt gggtctgctg gtgaatgatt acccgacctc cccatacgcc caggcccccg
     1081 ttgtggaacg gaccgggatt cgggcgatcc tggcagagcc gctcctgtac cgggggcaac
     1141 tcctcggggt cctcgaactg ctcaacgagc ctccccggga gggctttacg gaagaggacc
     1201 gacagctcct cgcgctgttt gccgcccagg ccgccatcgc catcgagaac agccgcctgt
     1261 acgaagcggt ccgccagcag gccgccgtgc tggaagaacg ggtccgagac cgtactcagg
     1321 aactggcggc ggcgaaccag cagctcaagg cggcctcccg acacaagtcc gaattcctgg
     1381 ccaacatgtc ccatgagctg cggacccctc tgaactccat cctcggcttc gcccaggtcc
     1441 tccaggaaca gaccaaggaa gtgctgggga ccaagcaggc tcgtttcctc acgaacatcc
     1501 ttaccagcgg ccagcacctc ctccagctca tcaacgatat cctggatatc agcaaggtcg
     1561 aggcaggaaa gttcatcctc cagcccgaac ccatcccggt cccggccacc ctcgaggaca
     1621 tcctggtcat tgctcggggt ctcgcgaaca agaattctca ggttatccag gccgacatcc
     1681 cggccgacct tcccgcactg cacgccgatc cggtccgctt caagcaaatc ctgttcaatc
     1741 tgttgagcaa cgccgtgaag ttcaccccca agggcggcac catcacgctc accgcgcgac
     1801 aagtggacgg tgaatcggag caggggagaa tcggagaaac ggaaacgcaa ccacccggcg
     1861 acggcgtccc cccgcgtggc cgttcccccg atgccctaat ttgcggagag agcgcgtacc
     1921 tggagatttg tgtccaagac accggcatgg gcatcaaggc ggacgacctc ccccgactct
     1981 tccaggagtt cgtccagctc caagctagtg cgaccaagga gaacgagggc accggcttgg
     2041 gcctcgccct gaccaagcgc ctggttgagt tgcaaggcgg ccgaatctgg gccgagtcgt
     2101 ccggggaggg tagtggaagc gtctttaccg tcctgctgcc cttgagaggt tcggggcgat
     2161 gatgggcgcg gagaatctgg aggcgctcct gcgggcgaca cgatccgtca t
//
LOCUS       DBNGZC010000022         1853 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_295275, whole genome shotgun sequence.
ACCESSION   DBNGZC010000022 DBNGZC010000000
VERSION     DBNGZC010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_295275"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="AC16G0_00340"
     CDS             complement(<1..344)
                     /locus_tag="AC16G0_00340"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5098097.1"
                     /translation="MTDYTSRFTLEGKRALVTGASKGIGAEIAAVFADAGADVAIVGR
                     DAAGLELTRQIVLREGRRCLVIEADLRTVEGPRFAGEEALEFFGTVDILVNNAGVVYV
                     SNFLNTSLEHWE"
     gene            complement(462..839)
                     /locus_tag="AC16G0_00345"
     CDS             complement(462..839)
                     /locus_tag="AC16G0_00345"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR5098098.1"
                     /translation="MTGMQVGYQDRYAARIKTIEEHIRLENLHDLDALMGTFGAAARY
                     EVEPFGDRHLGRDAVRALYDGIFQVLPDLHIAVKDRYCSEEALILEVAIRGTHSAEWM
                     AVAATGKRLESPPLCHLYLRRRR"
     gene            complement(879..>1853)
                     /locus_tag="AC16G0_00350"
     CDS             complement(879..>1853)
                     /locus_tag="AC16G0_00350"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5098099.1"
                     /translation="EVARHDSAAGWTLQLSLAVDSSLAWLPDEGAAEILDGHLEVIFG
                     TSFTPTGGRAIPVEGGYRLTGQWPFVSGAHDCRWFLFLPRLMDGDQPRLNTRGVPMQL
                     WAFLPADEASILDTWHTLGMRGTGSHDVAVSDLFVPERHTAPLVPLEQPGQAYQGPLY
                     RLTIWPPVALLAPPALGVARAAIDDLLELARAKTPTYTDATLRARQVVQRQVAEAEAT
                     LGAGRAYLYETFQENWDAAVQGAEITLERKLTMQLATTHANLAAATVVDLVHAAAGTS
                     AIRNEYRFQRYFRDVHTMTQHAFSSASRYESVGALMLGVESDWPFFAF"
BASE COUNT          332 a          617 c          585 g          319 t
ORIGIN      
        1 tcttcccagt gctccaggga ggtattcagg aagttgctca cgtacacgac gccggcgttg
       61 ttgacgagga tgtccacggt gccgaagaac tccagggcct cctcaccggc gaagcgaggc
      121 ccctcgaccg tccgcaggtc agcttcgatg accaggcagc ggcggccctc ccgcaggacg
      181 atttgcctgg ttagttccag ccctgccgcg tctcggccca cgatggcgac gtccgccccc
      241 gcatcggcga agaccgcggc gatctccgcc ccgatcccct tggacgcccc ggtgaccagc
      301 gcccgcttcc cctcaagggt gaaccggctg gtgtagtctg tcatccctgt cacctgatcg
      361 cgttatcagc ggacgcatag aaggccgtcc cgccgaccga agggacgccc atctgttcga
      421 ggatcatcgc tcggtcgaag tagatccttt cccccgccag cttatcgtcg ccgtcgaagg
      481 tatagatggc acagaggggg ggactccagc cgcttgcccg tcgccgcgac ggccatccac
      541 tcggcggagt gggtgccacg gatggcgacc tccaggatca gtgcctcctc ggagcagtag
      601 cgatccttca ccgcgatgtg gaggtccggc aggacctgga agatcccgtc atagagggcc
      661 cggacggcgt cccgaccgag gtgccgatcc ccgaagggct cgacctcgta gcgtgccgcc
      721 gcgccgaagg tccccatcag agcatcgaga tcatggaggt tttcgaggcg gatgtgctcc
      781 tcgatggtct tgatccgggc ggcatatcgg tcttggtatc ccacctgcat tccagtcatc
      841 gtgacctccg aagtggatgt attcgatcaa acgcaggttc agaaggcaaa gaagggccag
      901 tctgactcca ctccaagcat cagtgccccg accgattcat agcgactggc cgaactgaat
      961 gcgtgctgtg tcatcgtgtg cacatcccta aagtaccgct ggaacctgta ctcgttgcgg
     1021 atcgccgacg tgcctgccgc agcatggaca aggtctacca ccgtcgccgc ggcaaggttg
     1081 gcgtgagttg tggcaagctg catcgtcaac ttccgctcca gggtaatctc tgcgccctgg
     1141 acggccgcat cccagttctc ctggaaggtt tcatacaggt aagccctgcc tgcaccgagg
     1201 gtggcttcag cttcggccac ttgtctttgc accacctgac gtgctctcag ggtcgcgtcc
     1261 gtgtaggtcg gcgttttcgc gcgcgccagc tccagcaggt cgtcaatcgc tgcccgggcg
     1321 actcccaagg cagggggagc caggagcgcg accggaggcc agatggtcag gcgatacagc
     1381 ggaccctggt aggcctgacc gggctgctcc aggggcacca acggcgccgt gtgccgctcc
     1441 gggacgaaaa ggtcggacac ggcgacgtca tggctgcccg tgccgcgcat gcccaaggta
     1501 tgccacgtat ccaggatgct ggcctcgtca gccggcagga acgcccacag ctgcatgggg
     1561 accccccggg tgttgaggcg gggctggtcc ccgtccatga ggcgtgggag gaacaggaac
     1621 cagcgacaat catgggcgcc gctgacgaag ggccactgcc cggtcaggcg gtagccgcct
     1681 tccaccggga tcgcccgacc gccagtggga gtgaacgatg tcccaaagat gacctcaaga
     1741 tgaccatcca ggatctccgc cgccccctca tccggcagcc aggccaggga cgagtccaca
     1801 gcgagcgaga gctgcaaggt ccagccggcg gcgctatcgt ggcgcgccac ctc
//
LOCUS       DBNGZC010000023         2169 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_99743, whole genome shotgun sequence.
ACCESSION   DBNGZC010000023 DBNGZC010000000
VERSION     DBNGZC010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_99743"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1125
                     /locus_tag="AC16G0_00355"
     CDS             <1..1125
                     /locus_tag="AC16G0_00355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5098100.1"
                     /translation="PVVERASRGLAKHFPGQNSVVLVVDAGSRDGTPDAVREWKAGAP
                     PLPLVQLVALAGPPVEGRAIHAFLDAARRLEARACALLDADLSGLAPDWVERLLQPVL
                     REEADYASPAYTRSPPEGTLTTNLLAPMTRALYGKRIQQLLGGCLALSQAAVRQYLEA
                     DVWEGDLATHGIRMWLPTEALASGRKVVEVPLGRKIVSPGTREPDLAATLVHTVGPLF
                     SLMGRYASVWQDIRGSRPLPMLGDPPDLLWETREVHLDRMVRAFRLGLKDLLPVWEQV
                     MPDETLAQLYPLGILPAEEFRLPPSLWARVVADFALAYHERRLPREHLLRALMPLYLG
                     RVASFLHEARATPSHRLQDLLEAIGGAFESEKESLQSRWR"
     gene            1129..1830
                     /locus_tag="AC16G0_00360"
     CDS             1129..1830
                     /locus_tag="AC16G0_00360"
                     /inference="COORDINATES: protein motif:HMM:NF017373.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR5098101.1"
                     /translation="MRETWTEILVTSFQEVMRRLAQVTPQILAMLALIVLGLTVGAAA
                     RRLAARVLQAVDFDRRCARWGLTAALTRAGFRESPTLLTGRLIFWMIFLVALLMGIQA
                     LEMPATAGLVGMALRFLPDLIVAALVMVTGWVLANFLSQAALIGAVNAQVAGAPLIAG
                     AVRWIVLVFAGAAALTQLGIAREMVLLAFGIAFGGTVLALALAFGLGGKDLAREILES
                     RLRKHDEDTDRLTHV"
     gene            1927..2169
                     /locus_tag="AC16G0_00365"
                     /pseudo
     CDS             1927..2169
                     /locus_tag="AC16G0_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221481.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
BASE COUNT          305 a          737 c          745 g          382 t
ORIGIN      
        1 cccgtggtgg agcgggcatc caggggtctg gcgaagcact ttcccggcca gaactcggtc
       61 gtcctcgtcg tcgacgccgg ttctcgggac ggcactcccg acgcggtccg ggagtggaag
      121 gcaggcgccc cgcccctccc gctggtgcag ctggtggcct tggccggacc ccccgttgag
      181 gggagggcta ttcacgcctt tctcgacgcg gcgaggcgtc tggaggcccg cgcctgcgcc
      241 ctcctcgacg cggacctgag cggcctggcg cccgattggg tggagcgcct gctccagcct
      301 gttctccggg aggaagcgga ctacgcctcc cccgcctaca cccgcagtcc tcccgaaggg
      361 acgctcacga caaacctgct ggcccccatg acccgcgccc tgtacgggaa acggatccag
      421 cagctcctgg gcggctgcct ggcattgtcg caggccgcgg tccggcagta tctcgaggcc
      481 gatgtgtggg agggcgacct ggccacccac ggcatccgga tgtggctgcc cacggaagcc
      541 ctggcctcgg gccgcaaggt ggtggaagtc cccctgggtc gaaagatcgt gagcccggga
      601 acccgggagc cggacctagc cgccacgctg gtgcataccg tcggcccgct gttcagcctg
      661 atggggcggt acgcctcggt gtggcaggat attcgcggga gccgtcccct ccccatgctg
      721 ggagacccgc ccgatctctt gtgggaaacc agggaggttc atctggaccg gatggtccgg
      781 gcgttcagac tgggtttgaa agatctgctg cccgtatggg agcaagtcat gccggacgag
      841 accttggccc agttgtatcc cctggggatc ctgcccgcag aggagtttcg attgccaccc
      901 tccctctggg cccgcgtggt tgccgacttc gccctggcct atcacgagcg ccgtctgccg
      961 cgggagcatc tgctgcgggc gctgatgccg ctctacctcg gacgggtggc ctcttttctt
     1021 cacgaggccc gggccacacc gtcgcatcga ctccaggacc tgctggaggc gatcggcgga
     1081 gccttcgaat ccgaaaagga atccctgcag agccgatgga ggtaagccat gcgagagacg
     1141 tggaccgaaa tcctcgtcac ctcgttccag gaggtgatgc ggcgactggc acaggtcacg
     1201 ccccagatcc tggccatgct ggcgctgatc gtcttgggac tgaccgtcgg ggcggcggct
     1261 cggaggttgg cggcgcgggt cctccaggcg gtcgacttcg atcgccgctg tgcgcgctgg
     1321 gggctcacgg cggccctcac ccgggcgggc ttccgggagt ctccgaccct gctgacgggt
     1381 cggttgatct tctggatgat cttcctggtg gcgctcctca tggggatcca ggcgctggag
     1441 atgccggcca ccgcggggct ggtcggcatg gcccttcgct tcctgcccga cctcatcgtc
     1501 gcagccctgg tcatggtcac gggatgggtc ctggccaact tcctgagtca ggccgccctg
     1561 atcggcgcgg tgaatgccca ggtggcgggc gcgcccctga tcgccggtgc cgtgcggtgg
     1621 atagttctcg tcttcgccgg cgccgcggct ctcacccagc ttggcatcgc ccgggagatg
     1681 gtcctcctcg ccttcggcat cgccttcggc ggcacggtcc tggccttggc cctggccttc
     1741 gggctggggg gcaaggacct ggcccgggaa atcctggaga gccggctgcg aaagcatgac
     1801 gaggatacgg atcgcctgac ccacgtgtag ccccccagca cgggtctcaa cgggctagga
     1861 gacggctgcc catggcatac gcgcccatcg aggactacgg gatcatcggc gacctccata
     1921 cggtggcgct ggtgggagat ggtcaaccgc tcggccttgg ttttgaagct cctcaccttc
     1981 gaaccgacgg gggccatcgt ggcggcgccc accagcagcc ttcccggcgg cctgggcggg
     2041 ggacgcacct gagactaccg gtacacgtgg atccgagatg ccagcttcac cctctacgcc
     2101 ttgctgcgga tcggcttcac tcgcgagggc caccacttca tggactggct ccaggcgcga
     2161 tcgcgcgag
//
LOCUS       DBNGZC010000024         2951 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1273865, whole genome shotgun sequence.
ACCESSION   DBNGZC010000024 DBNGZC010000000
VERSION     DBNGZC010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1273865"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="AC16G0_00370"
     CDS             complement(<1..512)
                     /locus_tag="AC16G0_00370"
                     /inference="COORDINATES: protein motif:HMM:NF013213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR5098102.1"
                     /translation="MELFDAHAHLGSEEFAADLPAVLARAEEAGVCGILAVGETLEEA
                     ERILTLAERHPLIRPAAGLYPTILDQYAAEAMVAFVQTHADRLVALGEVGLDHWVVKD
                     EAGWKTQEEILARFVVLSNALDLPLNLHSRSAGRHAIGFLKERGARRVLMHAFDGRAS
                     AAAEGVRAGF"
     gene            complement(535..2343)
                     /locus_tag="AC16G0_00375"
     CDS             complement(535..2343)
                     /locus_tag="AC16G0_00375"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR5098103.1"
                     /translation="MANQTLSAVGVRWDLSDLFASHDDPRIRETLADCRARGEAFARR
                     YRGSINVSGGPEPAYLLGAIRELEELQEALGRVGAFAGLLYATDTSRPEYRDLRERVE
                     LQATEIGNLVLFFELEWMGLPDDAADGLMGAPILAGYRHYLRRSRRFRPHMLSEAEEQ
                     LLNERDNTGRRAFARLSTELTTSLSFPLEQDGKIQPLTMSEILALVHHTDREVRRRAH
                     EGLFEVLSKHDLVLTSTYDTLLQDHLTMDRLRHFPHPMAERHLSNEIDEEAVEQMLRV
                     TEANYGIAHAYFRVKARLLGLPLLALYDQYAPVSQELPSYSFDRARDVVLEAFGAFTP
                     SFREIAEQFFRRRWIDAEVRQGKQGGAFCSSPSPALHPYVLCNHTDNMRDVMTLAHEL
                     GHGLHGWLARKQTLFNYDTPLTLAETASVFGEFLVFDHLVRTQKDPRVQLALLCGKIE
                     DTCATVFRQNVLTRFEQAVFERRKAGRLTTEGVCQAWLDANRPYYHNAVEMTDGYRWG
                     WSYIPHFIHTRFYCYSYVFGELLVLSLYRRYKEEGASFIPKYVRLLEAGGSDAPESLL
                     QPLGVDFHDPAFWQKGFDELQSLVERAENLARDLGS"
     gene            complement(2414..>2951)
                     /locus_tag="AC16G0_00380"
     CDS             complement(2414..>2951)
                     /locus_tag="AC16G0_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098104.1"
                     /translation="EGRQTMHTLATGPVRRQIRMTLLALAGMVLAACSTQGTYGPLVA
                     GKPIYPPAVFAHRVATSDVTIYWNCSQPEPGLLQLDGIAQNIGGRDVRFLELHLTAVD
                     AKDRNILEAAAAVPDIVLYTNQLSPFHLDLRTPGTEVRFDLFYQYSLGSRLSKQDMQF
                     MARDVCSPTQHRVTNMGG"
BASE COUNT          522 a          986 c          950 g          493 t
ORIGIN      
        1 aagaagcctg cccgaacacc ctccgccgcc gccgaggccc tgccgtcgaa ggcgtgcatc
       61 aagacgcggc gggccccccg ctccttcagg aagccgatgg cgtggcgccc cgccgagcgc
      121 gaatggaggt tcagcgggag atcgagcgca ttggacagga ccacgaatct cgccaggatc
      181 tcctcctgcg tcttccagcc ggcttcgtcc ttcaccaccc agtgatccaa gccgacctct
      241 cccagcgcga ccagcctgtc ggcatgggtc tggacgaacg caaccatcgc ctcggccgca
      301 tactggtcga ggatcgtcgg gtacaatccg gcagcgggcc gaatcagggg atgtcgctcc
      361 gcgagggtga gaatccgctc cgcctcctcc agggtctctc ccacggccag gatgccgcag
      421 acgcccgcct cctcggcccg cgccagcacc gccgggaggt cggcggcgaa ctcttccgac
      481 ccgagatggg catgagcgtc gaacagttcc acggcttccc tgctcctcag tcccctagct
      541 ccccagatcc cgtgccaggt tctccgcgcg ctcgaccaga ctctgcagct cgtcgaagcc
      601 cttctgccag aaggccgggt cgtggaagtc cacacccaac ggctggagca aggactcggg
      661 cgcgtcggag ccacccgcct ccaagaggcg gacgtacttc gggatgaagg acgccccctc
      721 ctccttgtag cgccggtaca acgaaagcac caggagctcc ccgaacacgt agctgtagca
      781 ataaaaccgc gtgtgaatga aatgggggat ataggaccac ccccaccggt agccgtctgt
      841 catttccacc gcattgtggt agtaggggcg gttcgcgtcg agccaggcct ggcacacgcc
      901 ctcggtcgtc aggcgccccg ccttccggcg ctcgaagacc gcctgctcga aacgggtgag
      961 gacattctgc cggaagaccg tggcgcaggt gtcctcgatc ttcccgcaga gcagggccaa
     1021 ctgcacgcgg ggatccttct gcgtgcgcac caggtggtcg aagaccagga actccccgaa
     1081 caccgaggcc gtctcggcca gggtgagcgg cgtgtcgtaa ttgaacaggg tctgcttccg
     1141 cgcgagccac ccgtgcaacc cgtggcccag ctcgtgggcc agggtcatca cgtcccgcat
     1201 gttgtcggtg tgattgcaca gcacgtacgg atgcagcgcc ggcgaggggg aggagcagaa
     1261 ggcgccgcct tgcttgccct ggcggacctc ggcatcaatc cagcgccgcc tgaagaactg
     1321 ctccgcaatc tcccggaaag agggcgtgaa ggccccgaag gcttccagga ccacgtcccg
     1381 cgcccggtcg aaagagtacg acggcagctc ctggctcacc ggcgcgtact gatcgtagag
     1441 cgcgaggagc ggcaggccga gcaatcgtgc cttcacccgg aaataggcgt gggcgatgcc
     1501 atagttcgcc tcggtcacgc gaagcatctg ctccacggcc tcttcgtcga tctcattgct
     1561 caggtggcgc tcggccatgg ggtggggaaa gtgacgcagg cggtccatgg tcagatgatc
     1621 ctgaagcagg gtgtcgtagg tggacgtcag caccaggtcg tgcttggaca gcacctcgaa
     1681 caagccctca tgcgcccgcc gccgaacctc cctgtccgtg tggtggacca gcgccaggat
     1741 ctcgctcatg gtcagcggct ggatcttgcc gtcctgctcc agggggaagc tgagactggt
     1801 cgtgagctcc gtggagaggc gggcgaaggc gcgacggccg gtgttgtccc gctcgttgag
     1861 gagctgctct tcggcctccg agagcatgtg aggacggaac cgtcgcgacc ggcgcaggta
     1921 gtgacgatac ccggccagga tcggggcacc catgaggccg tcggccgcat cgtcggggag
     1981 acccatccac tccagctcga agaacaggac caggttgccg atctcggtgg cttgaagctc
     2041 gacccgctcc cggaggtcgc ggtattccgg tcgcgaggta tccgttgcgt agaggaggcc
     2101 ggcgaaggcg ccgacccgtc ccagtgcctc ctgcagctcc tccagctccc gaatcgctcc
     2161 aagcaggtat gccggctccg gtccccccga gacgttgatg ctgccgcggt accgccgggc
     2221 aaaggcctcg ccgcgcgccc ggcagtctgc cagggtttcc cggatgcgcg ggtcgtcatg
     2281 ggaggcgaac agatcgctga ggtcccagcg gaccccgacg gcgctgagcg tctggttggc
     2341 cacgctgcac tcctctaggg tttgcgggga aagcggatgc tgagcggaag actcatcgga
     2401 ttcccgagcg gcgtcatcca cccatatttg tgactcggtg ctgggtcggc gagcagacgt
     2461 ctctggccat gaattgcatg tcctgcttcg acaaacggct gcccaggcta tactgataga
     2521 agagatcgaa ccgcacttcg gtgcccggag tccgcaggtc caggtggaac ggggaaagct
     2581 ggtttgtgta gagcacaatg tccggcacgg cggcggcggc ctccaggatg ttgcgatcct
     2641 tggcgtccac ggccgtcagg tgaagttcca ggaaccggac atcccgtccc ccgatattct
     2701 gcgcgatccc atccagttgc agcaaacccg gctcgggctg cgaacagttc cagtaaatcg
     2761 tcacatccga tgttgccacc cgatgggcga acacggcagg tggatagatc ggctttcctg
     2821 ccaccagggg tccgtacgtc ccctgtgtgc tgcaggccgc cagaaccatc cccgccaggg
     2881 ccaggagcgt catccggatc tgcctgcgaa ccgggccggt cgccaacgtg tgcattgtct
     2941 gccttccttc c
//
LOCUS       DBNGZC010000025         3190 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_980311, whole genome shotgun sequence.
ACCESSION   DBNGZC010000025 DBNGZC010000000
VERSION     DBNGZC010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_980311"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="AC16G0_00385"
     CDS             <1..182
                     /locus_tag="AC16G0_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098105.1"
                     /translation="RDSIKDARHLGYAGPPSSKASEALAKFIIVDMFAKAIQGTSPEE
                     ALAWATGELKKIYAA"
     gene            264..1250
                     /locus_tag="AC16G0_00390"
     CDS             264..1250
                     /locus_tag="AC16G0_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5098106.1"
                     /translation="MVAPGGSTSVPVVVVPSRSITSGLARLRRATATERREWRFALML
                     LMPAVLMIGTFVAYPFIRGIWLSLTDTVVGREGEFVGLQNFAKLWDDSIFRRAAQNTV
                     FYTAAATVLKLIMGMVVALMLNNLLGFKRILRASMLLPWIVPTVLSTLAWKWMLDPTF
                     SILNWVLFQLGFIRFRVDWLGGPVMALWSVILVNVWRGTPFFAITLLAGLQTISPEVY
                     EAASIDGANAWQRFWTVTWPLLMPVTMVVMLFSVIQTFADFQLVYILTGGGPANSTHL
                     FATYAYQVGVATGKLGEGAAASLAMFPVLFGIVLFQLWYIRRTESGSKKRGA"
     gene            1256..2107
                     /locus_tag="AC16G0_00395"
     CDS             1256..2107
                     /locus_tag="AC16G0_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007681665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5098107.1"
                     /translation="MVAGGRWKRWVFLYLPLGAFVVITLFPFYYMAVTSIRPDREMYI
                     PWNRPNFAPFWTLQPTLEHFTLLFRETLFLRWLLNTLIISVLSTGIALFCGLLAAYAL
                     ARLRFPLGGTLGTSIFVTYLVPQTLLFIPLGEVIRSFQLSDSLWSLIFTYPTFLIPFC
                     TWLMMGYFRTIPRELEECAQIDGATRIQAMVRIIFPVAMPGILSAGIFAFTHAWNEFI
                     YALVFLSSATNKTVPVGVVSELVRWDTFFWGPLMAGALLGSVPVAFIYSFFVENYVSA
                     MTGAVKG"
     gene            complement(2516..2743)
                     /locus_tag="AC16G0_00400"
     CDS             complement(2516..2743)
                     /locus_tag="AC16G0_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014778214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098108.1"
                     /translation="MSLFVICINNDSNRASLILGKVYRALPDAQAEAHKMFRVLDEDK
                     SEPDGYLYPASMFAPIELPEAAARALMAASD"
     gene            complement(2740..2973)
                     /locus_tag="AC16G0_00405"
     CDS             complement(2740..2973)
                     /locus_tag="AC16G0_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006623181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098109.1"
                     /translation="MEWELVSEIRDIETIATGRGLFIRRYLERAYGKGRWRKMKGRAT
                     VRLADGTICEAEIHWYEAHGVGRRDFKVKRVIR"
BASE COUNT          505 a         1015 c          978 g          692 t
ORIGIN      
        1 tccgggattc catcaaggac gcgcggcacc tcgggtacgc aggaccgccg agttcgaagg
       61 cttccgaggc gctggcgaag ttcatcatcg tggacatgtt cgccaaggcg atccagggca
      121 cctcgccgga agaggcgctc gcctgggcga ctggcgaatt gaagaagatc tacgccgcct
      181 gagatcaatc gtctcccggc ccggggtctc gcgggaggcc cccaggtcag ctcctctcca
      241 ccccgttgtg ggagattgcc gccgtggttg caccgggagg gagcaccagt gtgccggtcg
      301 tggtggtgcc gagccgctcc atcacttccg gtctcgcccg tcttcgacga gcgaccgcca
      361 cggagcggcg cgagtggcgg ttcgccctca tgctcctcat gcctgcggtc ctgatgatcg
      421 gaaccttcgt ggcctacccg ttcatccggg gcatctggct gagtctcacg gacaccgtgg
      481 tcggccgtga gggagagttc gtcggactcc agaacttcgc caagctctgg gacgacagca
      541 tcttccggcg ggcggcgcag aacaccgtct tctacacggc ggcggccacg gtgttgaagc
      601 tcatcatggg gatggtggtt gcgctgatgc tgaataacct cctcgggttc aaacgaatcc
      661 tgcgggcctc catgctgctc ccgtggatcg tcccgacggt gctctcgacg ctggcctgga
      721 agtggatgct cgacccgacc ttcagcatcc tgaactgggt cctgtttcaa ctgggattca
      781 tccggttccg ggtggactgg ctcggggggc cggtgatggc cctgtggtcg gtcatcctgg
      841 tcaacgtctg gcgcgggacg ccgttctttg ccatcaccct gctggccggg ctccagacca
      901 tcagccccga ggtgtacgag gccgcctcca tcgatggggc caacgcctgg cagcgattct
      961 ggaccgtgac ctggcccctg ctcatgccgg tgacgatggt ggtcatgctg ttctcggtga
     1021 tccagacctt cgccgacttc cagctggtgt acatcctcac cgggggcggc ccggcgaaca
     1081 gcacccacct cttcgccacc tatgcctacc aggtcggcgt ggcgacggga aagctgggcg
     1141 aaggcgccgc cgcctccctg gccatgttcc cggtgctctt cgggatcgtg ctcttccagc
     1201 tttggtatat ccgccgcacc gagagcggca gcaagaagcg aggtgcctga tggcgatggt
     1261 cgcggggggg cgctggaagc ggtgggtgtt cctgtatctg ccgctggggg cgttcgtcgt
     1321 catcaccctg tttcccttct actacatggc ggtgaccagc atccggccgg accgggagat
     1381 gtatatcccg tggaaccggc cgaactttgc tcccttctgg acgctccagc ccacgctgga
     1441 gcacttcacc ctcctcttcc gcgagacgct cttcctccgc tggctcctca acacgctgat
     1501 catctcggtg ctgtccacgg gcatcgccct gttctgcggt ctcctggcgg cctacgccct
     1561 ggcccggctg cggttcccgt tggggggtac cctcggcacc agcatcttcg tgacgtacct
     1621 ggtcccgcag acgctcctgt tcattccctt gggggaggtc atccgtagct tccaactgag
     1681 cgactcgctc tggtcgctga tcttcacata ccccaccttc ctcattccct tctgcacctg
     1741 gctgatgatg gggtacttcc ggaccattcc ccgggagctg gaggagtgcg cccagatcga
     1801 cggggccacc cggatccagg ccatggtgcg gatcatcttt cccgtggcga tgcctggaat
     1861 cctctcggct ggcatctttg ccttcacgca cgcctggaac gagttcatct acgccctggt
     1921 cttcctctcc agcgcgacca acaagaccgt cccggtggga gtggtgagcg agcttgtcag
     1981 gtgggacacg ttcttctggg ggccgctcat ggccggcgcc ctgctcgggt cggtgcccgt
     2041 ggccttcatc tattccttct tcgtcgagaa ctacgtgagc gccatgaccg gggcggtgaa
     2101 ggggtagggg aagggttatc ctaggccacc accgctgctt cgatcttcgg cccgctgcgg
     2161 tgatcccaac catgaggaac ccttcaccgg ttgctcaaca ggcgattgga tcgatccggc
     2221 taggctccga tagaatttgc tggtggctaa tgtgccggca tgggctcgaa ggccatgtct
     2281 gcactcggca cagggacgga gcagggctgg tgttttctcg attgtttccg cgtggtcgat
     2341 gaaggcgatg gggcgtcacc gaggagagtg cgaatcaacc cacggcctac tcgcggaaga
     2401 tggattcccc cctgctggca aggaggagac gtcactacgg gcgacagtcg gaccaccgag
     2461 actgaggcat ctcgcatcat ggccagccgc cgtaggcggc gagtccgtat tgggtttagt
     2521 cgcttgctgc catcagggca cgggccgcgg cttctggcaa ctcaatgggt gcaaacatgg
     2581 aggctgggta gagatagccg tctggttccg acttgtcctc atccaggacg cggaacatct
     2641 tgtgcgcttc ggcctgtgca tctggcagtg cgcggtaaac cttgcccaag atcaggctgg
     2701 ctcgattact atcgttattg atgcagatca caaaaaggct catcggatta ccctcttgac
     2761 cttgaaatcc ctgcgcccta cgccatgcgc ttcgtaccag tgtatctcgg cttcacagat
     2821 tgtgccgtct gcaagtcgca cagtcgccct gcccttcatc ttgcgccagc ggcctttacc
     2881 gtaagcgcgt tccagatagc gcctgatgaa gaggcctcgg cctgttgcga ttgtctcgat
     2941 gtctctgatc tcacttacta gctcccattc tatcatcgtc caccgtagat gagtgacagg
     3001 aatttcattt tcgggcttgc gcggccattc ccgcacgctg cgccacttcc gggccaccgc
     3061 tgtcaggctc gctgcggcgc ctggactctc tcggagcatc cagccatgta ggtgggaatg
     3121 tcaaggggga atacgttgcc atgttgccat gctagccgta gtgtcatacc aacgtgttcc
     3181 ccagcagttg
//
LOCUS       DBNGZC010000026         4472 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1860895, whole genome shotgun sequence.
ACCESSION   DBNGZC010000026 DBNGZC010000000
VERSION     DBNGZC010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1860895"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="AC16G0_00410"
     CDS             <1..964
                     /locus_tag="AC16G0_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012267.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR5098110.1"
                     /translation="VYAPHEFDHGLGARRAIYIPHANAIPQVAILDPEYCVFDGNCAK
                     ACPRDCIDFTQQAEEQSVDVGAVIVASGLNITPMGAKKEYGGDRYVNVMNPLAMERVQ
                     SSNGPYGHVLRPSDGKVPKSVAYVQCAGSRDASIGVPYCSRVCCMYALKQAIMIKHQL
                     HDTEVTIYHMDIRAFGKGYEQFYRRAMAEGIRVVKAKVAKITETQTHDLIVRVELIDE
                     GGRVADIQHELVVLSQGLIPAWHPGDIIPIGEARDGFFDVPELATLPARSTCEGIFLA
                     GVSTGPKDIPDAIIEAGAAAMEAAMYLDRTRTGQAAAAGSGHRA"
     gene            990..1286
                     /locus_tag="AC16G0_00415"
     CDS             990..1286
                     /locus_tag="AC16G0_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098111.1"
                     /translation="MNGTKRLEWREALKVFRERMGGLTDAKKAYFKADRDTRKALREA
                     LRFGPKTIPELSQQLNIAGEKVTWYVMALKRYGEIVEAGQAGEYFRYGLKESER"
     gene            1283..2446
                     /locus_tag="AC16G0_00420"
     CDS             1283..2446
                     /locus_tag="AC16G0_00420"
                     /inference="COORDINATES: protein motif:HMM:NF024581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5098112.1"
                     /translation="MSVRVDTGVVSELAKYGGDTVSKCFNCGNCTAVCSLSKEDTVFP
                     RRYIRYTQLGLKGKMLESVDPWLCYYCGDCSDTCPRDAKPGKLMMASRRWLISMYDWT
                     GLSRLMYKREAWELGMLAIVALVVLGLFTLPSNFGFRLLASHPEARQTVALAYFAPKA
                     IVHWGDIVMAVLLAGLLLSNAARMTYYAMRTSHPVPLSAYLIELKQLILHAVTQKRWR
                     DCTTDNTKHWLRHVALVSGYGTMFVLVVVFLYWFQVEDATFHWTSLLGYYATLVLLGS
                     TLWIIRDRLEKKDQIHKDSDLADWLFVGLLLLTSLTGILLHLVRLLDLPMPTYVMYLV
                     HLMIAVPMLVVEVPFGKWAHMLYRPLAIYLSAVQARARQPHVSHVKAAPPLPA"
     gene            2549..3196
                     /locus_tag="AC16G0_00425"
     CDS             2549..3196
                     /locus_tag="AC16G0_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5098113.1"
                     /translation="MFKRMTGGDFSRPIPRIPGPKDIRRHIAYESELDPGFGERIARK
                     AYGEKLFSCIQCGTCSGTCPVSHYMDYTPRKIIAMIREGFRDEVLHCVTIWLCASCYS
                     CTVDCPREIKITDVMYALKQEAMSQGVYPGGFSIPVLAREFFRGVERGGRSNELPIML
                     RFFLRTNPLRMLQNAPVAWRLWRTGRLSLRREQTRQRDSVRTLLRAAKAAEESTP"
     gene            3193..4104
                     /locus_tag="AC16G0_00430"
     CDS             3193..4104
                     /locus_tag="AC16G0_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HIR5098114.1"
                     /translation="MSYWYYPGCSLKSNGSPYEQSLLAVFRELDTPLRELDDWNCCGA
                     TSYMSIDEVQAFSLAARNLALAEQASHEAPEKGAPQLVAPCSACFLVLTKTQRYIKQH
                     ENLARQVHTGLRAIDLDYQGVVKVRHPLDVFVNDIGLPRIRKAMKRSLDGLKVACYYG
                     CQVVRPFATFDSAHNPTSMDRLVETLDAKPIPWPLKTRCCGGTLTGTIRDVGLRLSQI
                     LLKDAAQRGAEVIITVCPLCQFNLECYQEEIVRTFPETTRLTVLYLTQLMGKAFGIPD
                     AALGMRRLFVSPEPAFARHQGGQPVHA"
     gene            4097..>4472
                     /locus_tag="AC16G0_00435"
     CDS             4097..>4472
                     /locus_tag="AC16G0_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide reductase"
                     /protein_id="HIR5098115.1"
                     /translation="MHDYSGNGTPRIGVYICHCGTNIAGVVDVVALAEYAAGLPGVVV
                     SREYKYMCSDPGQEMIQQDIRDHKLDRIIVASCSPLLHEHTFRHATAQGGLNPYFFQM
                     VNIRENVSWVNQDKQAATQKAKD"
BASE COUNT          822 a         1481 c         1400 g          769 t
ORIGIN      
        1 cgtgtatgcg ccccacgagt tcgaccacgg gctgggggcg cggcgggcca tttatatccc
       61 ccacgccaat gccatccccc aggtggcgat cctggacccc gagtactgcg tcttcgacgg
      121 caactgcgcc aaggcctgcc cccgggactg catcgacttc acgcagcagg cggaggagca
      181 gagcgtggac gtgggcgcgg tgatcgtggc cagcggcctc aacatcacgc ccatgggtgc
      241 gaagaaggag tacgggggcg accggtacgt caacgtcatg aaccccctgg ccatggaacg
      301 cgtccagtcc tccaacggac cctacggcca cgtcctccgg ccctcggatg gcaaggtgcc
      361 caagtcggtg gcctacgtcc agtgcgccgg aagccgcgac gcctccatcg gcgtgcccta
      421 ctgctcccgg gtctgctgca tgtacgccct caagcaggcc atcatgatca agcaccaact
      481 gcacgacacg gaggttacga tctaccacat ggacatccgg gccttcggca agggctacga
      541 acagttctac cggcgcgcca tggccgaggg catccgggtg gtcaaggcca aggtggccaa
      601 gatcaccgag acgcagaccc atgacctcat cgttcgcgtg gagctcatcg acgaaggcgg
      661 gcgggtggcg gatatccagc acgagctggt ggtcctgagc cagggcctga tccccgcctg
      721 gcaccccggg gatatcatcc ccatcggcga ggccagagac gggttcttcg atgttcccga
      781 gctggccacg ctgccggcga gaagcacgtg tgagggaatc ttcctcgcgg gtgtatccac
      841 cggaccaaag gacatcccgg acgccatcat cgaggcgggg gccgccgcca tggaggcggc
      901 gatgtatctg gatcggaccc ggacgggtca ggcagccgcg gcgggaagcg gtcatcgcgc
      961 gtgacggaac cgcagccagg gggacagcca tgaacggaac gaagcggttg gaatggcgcg
     1021 aagcgctcaa ggtgttccgc gagcggatgg gtggcctgac cgacgccaag aaggcctact
     1081 tcaaggcaga ccgggacacg cgcaaggcgc tgcgggaggc cctccggttc gggcccaaga
     1141 cgatcccgga gctcagccag cagcttaata ttgccggcga gaaggtcacg tggtacgtca
     1201 tggcgctgaa gcggtacggc gagatcgttg aagccgggca ggcgggcgag tatttccgct
     1261 acggcctgaa ggagtcagag cgatgagcgt ccgcgtcgat accggggtgg tctcggagct
     1321 ggcgaaatac ggaggggaca cggtcagcaa gtgcttcaac tgtggaaact gcacggccgt
     1381 ctgctccctc tccaaggagg acacggtctt tcctcggcgg tacatccggt acacgcagct
     1441 tgggctgaag gggaagatgc tggagtccgt cgatccgtgg ctgtgttact actgcggcga
     1501 ctgctccgac acctgtcctc gcgatgcgaa gccaggcaaa ctcatgatgg ccagccgccg
     1561 ctggctcatc agcatgtacg actggaccgg cctgagccgg ctcatgtaca agcgagaggc
     1621 ctgggagttg gggatgctgg cgatcgtcgc cttggtggtc ctgggcctct tcacgctccc
     1681 gagcaacttc ggcttccgac tcctggccag tcaccctgag gcgcgtcaga cggtcgccct
     1741 ggcctacttc gcccccaagg cgatcgtcca ctggggcgac attgtcatgg cggtgctctt
     1801 ggcggggctg ctcctgagca atgccgcccg catgacctac tatgccatgc ggaccagtca
     1861 ccccgtcccg ctcagcgcgt acctgatcga gctgaagcag ttgatcctgc acgccgtcac
     1921 acaaaaacgc tggcgtgact gcaccaccga caataccaag cattggctgc gccacgtggc
     1981 cctggtgagc ggctacggga cgatgttcgt cctggtggtg gtcttcctgt actggttcca
     2041 ggtcgaggac gccacgttcc actggacgag cctgctgggc tactacgcca ccctcgtgct
     2101 cctcggcagc accctctgga tcatccggga ccgcctggag aagaaggacc agatccacaa
     2161 agacagcgac cttgccgact ggctgttcgt gggccttctg ctcctcacca gcctgacggg
     2221 catcttgctc caccttgtcc ggctgctcga cttgcccatg ccgacctacg tcatgtatct
     2281 ggtccatctc atgatcgccg tccccatgct ggtggtcgag gtgccgttcg gcaagtgggc
     2341 tcacatgctc tatcgccctc tggcgattta cctctcggcg gtgcaggcgc gcgcccgcca
     2401 gcctcacgtc tcccacgtga aggcggctcc gccgctcccg gcctaggcca gacgcccgtt
     2461 tggcacggcg gattgcatcg gaggactcgg aagcacgctg cacccacaag gggtgcgtgc
     2521 gacaaggtat gcgaggagga atcgacctat gttcaaacga atgaccggcg gggatttctc
     2581 ccgtccgatt ccgcggattc ccggccccaa ggatatccgc cggcacatcg cctacgaatc
     2641 agagctcgac ccgggattcg gcgaacgcat cgcccgcaag gcgtacggcg agaagctgtt
     2701 ctcctgcatc cagtgcggaa cctgcagcgg gacctgcccg gtcagccact acatggatta
     2761 caccccccgg aagatcatcg ccatgatccg ggaaggcttc cgcgatgagg tcctgcactg
     2821 cgtcaccatc tggctgtgcg cctcctgtta ctcctgcacc gtggattgcc cgcgggagat
     2881 caagatcacc gacgtgatgt acgcgctgaa gcaggaggcc atgtcccagg gggtctatcc
     2941 gggcggcttt tccatcccgg tgctggcgcg cgagttcttc cggggcgtcg agcgcggcgg
     3001 tcgcagcaac gagttgccca tcatgctcag attcttcctg aggacgaacc cgctgcggat
     3061 gctccagaac gcgcccgtcg cctggcgtct ctggcgcacc ggccgactgt ccctccggcg
     3121 ggagcagacg cgacagcggg atagcgttcg cacgctgctg cgtgcggcca aggcggcgga
     3181 ggagagtaca ccatgagcta ttggtactac ccgggctgct ccctcaagag caatggcagc
     3241 ccctacgagc agtctctcct cgccgtgttc cgggagctgg acacgcccct cagggaactg
     3301 gacgattgga actgctgcgg ggcgacgtcc tatatgtcca tcgacgaggt ccaggccttc
     3361 agtctggcgg cgcggaacct ggcgctggcc gagcaggcct cgcatgaggc tcccgagaag
     3421 ggcgcgcctc agttggtagc gccgtgcagc gcctgcttcc tggtattgac caagacccag
     3481 cgctacatca agcagcacga gaatctcgcc cgccaggtcc atacgggact ccgggcgatc
     3541 gatctggact accagggggt tgtcaaggtc cgtcatcccc tggacgtctt cgtcaacgac
     3601 atcggtcttc cgcggatccg gaaagccatg aagcgatccc tcgacgggct gaaggtcgcc
     3661 tgctactacg gctgccaggt ggtacgcccc ttcgccacct tcgactcggc ccacaacccc
     3721 accagcatgg accgccttgt ggagaccctg gatgccaagc cgatcccctg gccgctgaaa
     3781 acccgctgct gcgggggcac cctgaccggg accatccgcg acgtgggcct caggttgagc
     3841 cagatcctcc tcaaggacgc cgcccagcgc ggggccgagg tgatcatcac cgtctgcccg
     3901 ctctgccagt tcaacctgga atgctaccag gaagagatcg tacgcacctt cccggaaacc
     3961 acccgactca cggtcctgta tctgacccag ctcatgggca aggccttcgg catcccggac
     4021 gcggcgctcg gcatgcggcg attgttcgtg tcacccgagc cggccttcgc ccgccatcaa
     4081 ggagggcaac ctgtccatgc atgattacag cggaaacggc accccccgca tcggcgtgta
     4141 catttgccac tgcggaacaa atatcgccgg tgtcgtagac gtcgtggcgc tggccgagta
     4201 tgccgccgga ctccctggcg tggtggtatc gcgcgagtac aagtacatgt gctccgaccc
     4261 gggccaagag atgatccagc aggacatccg ggatcacaaa ctcgaccgta tcatcgtcgc
     4321 ctcctgctcg cctctcctcc acgagcatac gttccggcac gccacggccc agggcgggct
     4381 caacccctac ttcttccaga tggtgaacat ccgcgagaat gtctcgtggg tgaatcagga
     4441 caagcaggcc gccacccaga aggccaagga tc
//
LOCUS       DBNGZC010000027         2512 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2252134, whole genome shotgun sequence.
ACCESSION   DBNGZC010000027 DBNGZC010000000
VERSION     DBNGZC010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2252134"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1697)
                     /gene="nuoL"
                     /locus_tag="AC16G0_00440"
     CDS             complement(<1..1697)
                     /gene="nuoL"
                     /locus_tag="AC16G0_00440"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIR5098116.1"
                     /translation="MLKLVWLVPVLPLVGVVINWILGRWVRTRAHILGVGTTGLSFLI
                     GLAIFLQVAGGQTLNWDAYVWVPVQNLEATVGFQLDPLSAVMMLVVTFVGFLIHVYSV
                     GYMHGDPGYARFFTYLNLFMTSMLILVLANNYLLMFLGWEGVGLCSYLLIGFWYQKKS
                     ASDAGKKAFVVNRIGDAGFLLGMFLIWRTFGSLQYDQVFPMVHEVQDILQGPSVFGFS
                     LVTWLTLLLFVGAIGKSAQLPLYVWLPDAMEGPTPVSALIHAATMVTAGVYMVARSHA
                     LFSLAPFSMDVVASVGALTAIFAASIALVQTDIKRVVAYSTISQLGYMFLGAGVGAYA
                     TAIFHLATHAFFKALLFLGCGSVIHGLSGEQDVRKMGGLRKHMPVTAYTFLLASLANA
                     GIFPLAGFWSKDQILHAAIASDRPFLWFLGAAGAFCTAFYMFRLYFLTFEGESRVDHD
                     VAHHVHESPRVMTIPLVVLAVFTVGAGLFGYSPEFGVYYKLVRPVFEVEVVGGEALTH
                     GPSELLMAIISVAIAIAGIALAYQMYLRRPGMADRIAARFSGLYRLLLNKYWVDEIY"
     gene            complement(1882..2190)
                     /gene="nuoK"
                     /locus_tag="AC16G0_00445"
     CDS             complement(1882..2190)
                     /gene="nuoK"
                     /locus_tag="AC16G0_00445"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HIR5098117.1"
                     /translation="MIPLSHYVILGAIIFSLGVFGALTRRNAIAILMSIELMLNAVNL
                     NFIAFARYLPQTLLQGQIFAIFVITVAAAEAAVGLAIVLGLYRNFKTVNVDEINLMKW
                     "
     gene            complement(2199..>2512)
                     /locus_tag="AC16G0_00450"
     CDS             complement(2199..>2512)
                     /locus_tag="AC16G0_00450"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J family
                     protein"
                     /protein_id="HIR5098118.1"
                     /translation="IYVGGITILLLFAIMLTSRISAQSTRVINDQVLLSAIAAVGLLI
                     GLIYAGTRGIGALVAPPRIPETSSFLGRALLTTYAQPFEAVSVLLLAAMVGAIILARK
                     E"
BASE COUNT          549 a          764 c          816 g          383 t
ORIGIN      
        1 ttgtagatct cgtccaccca gtacttgttg aggaggagcc ggtacaaccc agaaaaccga
       61 gcggctatcc gatcggccat tccgggccgc cgcaggtaca tctggtacgc cagggcaatc
      121 ccggcaatcg cgatggcaac cgagatgatc gccatgagca gctcggacgg tccgtgcgtc
      181 agcgcctcac cccccacaac ctccacctcg aacactggcc tgacgagctt ataatagacg
      241 ccgaattcgg gtgagtagcc aaagagcccg gccccgacgg tgaagacggc caggacgaca
      301 agggggatcg tcatcacgcg gggggattca tgcacgtggt gggcgacgtc gtggtccacc
      361 cgcgactcgc cctcgaaggt caggaagtag aggcggaaca tgtagaaggc cgtgcagaag
      421 gctcccgccg cccccaggaa ccagaggaag ggccgatccg aggcgatggc ggcatgcagg
      481 atctggtcct tggaccagaa acccgccagg gggaagatcc cggcgttggc cagcgaggcc
      541 agcaggaagg tgtaggccgt gacgggcatg tgcttgcgca ggccccccat ctttcgcacg
      601 tcctgctcgc ccgagaggcc gtggatgacg gagccgcagc ccaggaacag gagggccttg
      661 aagaaggcgt gggtggccag gtggaagatg gctgtggcgt acgccccgac cccggccccc
      721 aggaacatgt aaccgagctg cgagatggtg gagtaggcca ccacccgctt gatgtcggtc
      781 tggaccaggg cgatgctggc ggcgaaaatg gccgtgagag cccccacgct tgccaccacg
      841 tccatgctga agggggccag gctgaagagc gcatgggacc tggccaccat gtagacgcct
      901 gcggtcacca tggtcgcggc gtggatcagg gcggagaccg gggtcggccc ttccatggcg
      961 tccggcagcc agacgtagag cgggagctgg gccgacttgc cgatagcacc gacgaacaag
     1021 aggagcgtca accaggtaac cagactgaac ccaaaaaccg agggaccctg gaggatgtcc
     1081 tggacctcgt gcaccatcgg gaagacttgg tcgtactgga gcgagccgaa tgtccgccag
     1141 atcaggaaca tccccagcag gaacccggcg tccccgatcc ggttgacgac gaaagccttc
     1201 ttgcccgcgt ccgacgccga tttcttctgg taccagaagc caatgagcag ataggagcag
     1261 aggcccaccc cttcccagcc caggaacatc aagaggtagt tgttcgccag gaccaggatg
     1321 agcatggagg tcatgaacag gttcaggtag gtgaagaacc gggcgtatcc gggatcgccg
     1381 tgcatgtagc cgacggaata gacgtggatc aggaacccca cgaaggtgac caccagcatc
     1441 atgacggcag acagggggtc gagctggaag ccgacggtag cctctagatt ctgcacgggg
     1501 acccagacat atgcgtccca attgagcgtc tgcccgccgg ccacctgaag gaagatcgca
     1561 agaccgatga ggaaggagag gccggtggtg ccgaccccca agatgtgggc gcgggtccgc
     1621 acccagcgac ccaagatcca gttgatcacc acccccacga gggggagtac cggcaccagc
     1681 cagaccagct tcaacatacg gggtccttag tcatcggcag tgaattcact gtcctcggcc
     1741 atcaacttcg gtcgacagag ccaatgaaag acggacaacg gacaaccgac aaccggccat
     1801 gtggtcatga cccttcatgt tccggtttgg tcggttgtca gttgtcggtt tcccatttga
     1861 aattccgccg ggtcaggcgg ctcaccattt catcaggttg atctcgtcca cgttcaccgt
     1921 cttgaagttg cgatacaacc ccagcacgat ggcgaggccc accgccgcct cggctgccgc
     1981 cacggtgatg acgaagatgg cgaagatctg cccctggagc agggtctggg ggaggtagcg
     2041 ggcgaaggca atgaagttca ggttcaccgc gttcagcatc agctcgatag acatgaggat
     2101 ggcgatggcg tttcggcggg tcagcgcccc gaacaccccg aggctgaaga tgatcgcccc
     2161 caggatcacg tagtgactca gcgggatcat cggaccccct attccttcct ggccaagatg
     2221 atggccccca ccatggccgc caggagcagg acggagaccg cctcgaaggg ctgcgcatac
     2281 gtcgtgagca gcgcccgtcc gaggaacgac gaggtctccg ggatgcgcgg cggggccaca
     2341 agtgcgccga tgccccgcgt cccggcgtag atcaggccga tcaggagacc caccgccgcg
     2401 atggcgctca ggagcacctg gtcgtttatc acccgcgtgc tctgggccga gatccggctg
     2461 gtcagcatga tggcgaacag gagcagaatc gtgatcccgc cgacgtagat ga
//
LOCUS       DBNGZC010000028         4057 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_686801, whole genome shotgun sequence.
ACCESSION   DBNGZC010000028 DBNGZC010000000
VERSION     DBNGZC010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_686801"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..508
                     /locus_tag="AC16G0_00455"
     CDS             227..508
                     /locus_tag="AC16G0_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008592560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HIR5098119.1"
                     /translation="MATLQVKGLDEGLYKALGARAKQDNRSISQEVVKLIQDFLARPG
                     GSAEDSTRAFLALCGSWDDPRSAREIAGSIRKSRRAGKRFAKGGGDVFA"
     gene            495..896
                     /locus_tag="AC16G0_00460"
     CDS             495..896
                     /locus_tag="AC16G0_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002740915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR5098120.1"
                     /translation="MYLLDTDTLIYALKGDPSVTRRLDEKAAQPKALSVITYGELFFG
                     AVKSSAPQANLAKVRRVTELFPVIEVSRAIMETFGSLKADLQKRGKAVEDFDLVIAST
                     ALVLSCTLVTNNERHFRNIPGLSVENWARPA"
     gene            1245..2066
                     /locus_tag="AC16G0_00465"
     CDS             1245..2066
                     /locus_tag="AC16G0_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HIR5098121.1"
                     /translation="MSIIAVSRGTFSGGEALAKGVAERLGYGCVSREVIHEAAERYGF
                     AVEELVAAMDKRPPLWERLAGKRTAYLICMRAALCDRALKGQLVYHGYLGHLLLPGIT
                     HVLRVRVIADMEFRIRAAMQQQQLARKEAEAYIEKVDKERRQWTRFLFDVAWDDPNLY
                     DIVLNLSHVSLAAACETVVRLAALSEFMPTSASMKAMQDLALGSRVAAACARDPRTRD
                     TNLEVTADDGIVTVTGTTQSPVVVGAVPMVARQVEGVRDVRAEVRLLREGRGGPA"
     gene            complement(2150..2707)
                     /locus_tag="AC16G0_00470"
     CDS             complement(2150..2707)
                     /locus_tag="AC16G0_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR5098122.1"
                     /translation="MAKVSKKPGTYLYPLPPSLITSGPAEKPNIITLAWMGTLCSEPP
                     IIGINIRPSRYSHGLVKQSGEFAVNVPTAEMVRVVDWCGTVSGHSEDKFAAMGLTPVP
                     TQVIRTAVIKECPLNIECQVVQTIHLGSHDLFLGKVVAVQVDESILDAQGEIDLTKAN
                     PLAYGGHAYWTLGQFVERQGYSASH"
     gene            complement(2752..3303)
                     /locus_tag="AC16G0_00475"
     CDS             complement(2752..3303)
                     /locus_tag="AC16G0_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HIR5098123.1"
                     /translation="MAAGGRTVVLGLGNPVLSDDGVGLAVAAELKRLLAVAPIRGVDV
                     LASTRAGFELIDLLRGYTRAVILDCILLPDARPGRVCRLTLDDVSGCARLVNAHEVSI
                     GTAFRLAERMGIPMPHEAVVIAVEAADTATLAEGLTPAVQAAVAPLAQEIYESLKRDE
                     PATDPLDTQDFSQRRAFYSPGDE"
     gene            complement(3310..3798)
                     /locus_tag="AC16G0_00480"
     CDS             complement(3310..3798)
                     /locus_tag="AC16G0_00480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HIR5098124.1"
                     /translation="MTQQQPRILVLGLGNDILGDDAVGLIAARRLRPHCSSQVDVVEA
                     AESGLGLLDLLEGRRHALLLDAILTGRHPAGTVLEFSQADFHGTIGPSPHYAGLPEVL
                     RLADCLAIPVPTDLCVLAMEVEDPYQIREGLSLTVDQALPGYVERARQILDGWQRARR
                     TP"
     gene            complement(3795..>4057)
                     /locus_tag="AC16G0_00485"
     CDS             complement(3795..>4057)
                     /locus_tag="AC16G0_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HIR5098125.1"
                     /translation="QGLIKKANLIVATQGNSARMAMGIEKSAKGLIHAGKVDDGLLNM
                     IEMAFRAYDPCHGCATHSLPGEMPLIVSIRDASGQVLQEIRR"
BASE COUNT          756 a         1202 c         1364 g          735 t
ORIGIN      
        1 ctctgccgca attgtaggcg ggaaagcctg cccccgcaac cggatagggg acttgtctgg
       61 caaatcagaa gagcaagcat gcggaattgg cgcaatgctt attccaatgt ctcgccggtg
      121 ccacccttcc gacgttcatg aaattagatt gcactttcat gaagcagcat gtatccttct
      181 tcgtaagcgt ttaggcaatt ccttactcgt cggggaggtg gtagacatgg caaccttgca
      241 ggtcaagggc cttgatgagg gcctttacaa ggcattgggt gcgcgcgcga agcaagacaa
      301 ccgctcgatc agtcaggagg tggtgaagct gatccaggat ttccttgcgc ggccaggtgg
      361 ttctgcggag gattcaaccc gggctttcct tgccctttgc ggctcgtggg acgacccgcg
      421 ctcggcgcga gagatcgccg gaagcatccg aaagagtcgg cgtgctggca agcgcttcgc
      481 caaggggggc ggcgatgtat ttgcttgata cggacacgct gatctatgcc ctcaagggcg
      541 accccagtgt gacgcgccgc ttggacgaga aggccgcgca gccgaaggcg ctttcggtga
      601 ttacctacgg ggagctattc tttggtgcgg tgaaatcatc ggcgccacag gccaatttgg
      661 cgaaggtacg tcgggtcact gaactttttc cggtaatcga ggtgtcgcgg gcgatcatgg
      721 aaacgtttgg ctcgctaaag gcagatctac agaagcgggg gaaggccgtg gaagatttcg
      781 atctcgtgat cgcctcaacc gcccttgttc tgagctgcac gctcgtgacc aataacgaac
      841 gtcatttccg caacattccc ggcctttcgg tggagaactg ggctcgccca gcctgaactc
      901 tccgatgaag gggagaggta tgcctgcagg aaagcagagc gatgcaagaa gtttcttgga
      961 aagggtcggg cgtcgttcgc tgactcttgg aggactcctc cagtcgattc gcctgtgtga
     1021 aaagatgtct caggccacca tcaccaagac acttggcatc tcagcctctc atctatgcca
     1081 tatcgagaag ggcaggagag gcggcagtca agccccggtg aatatgacaa atcgagccgg
     1141 tgtccctccc acaggggaac tgtcggtgat aggaagggca aatctggggg ggatggactt
     1201 ttcgaggatc gggtcgttgc tggcggatcg gaaaggagag gacgatgtcc atcattgccg
     1261 tttcccgggg aacgttcagc ggcggggagg cgttggccaa gggcgtggcc gagcggctgg
     1321 ggtatgggtg cgtcagccgg gaagtgattc acgaggcggc cgagcggtat gggttcgcgg
     1381 tggaggagct ggtcgccgca atggataaac ggccgccgct ctgggagcgg ctggcaggga
     1441 agcggaccgc ctatctcatc tgcatgcggg cggccctgtg cgatcgggcc ctgaagggcc
     1501 agctcgtcta tcacggatac ctggggcatc tcttgttgcc aggcatcacc cacgtcctgc
     1561 gcgtgcgggt gatcgccgac atggaattcc gcatccgggc ggccatgcag cagcagcagc
     1621 tcgcgcgcaa ggaggcggag gcatatatcg agaaggtgga caaggagcga cggcagtgga
     1681 ccaggttcct tttcgacgtg gcatgggatg accccaacct ctacgacatc gtcctgaacc
     1741 tcagccacgt gagcctggcc gccgcgtgcg aaacggtggt tcggttggct gccctatccg
     1801 agttcatgcc gaccagcgcg tcgatgaaag ccatgcagga cctcgcgctg ggcagccggg
     1861 tcgcggccgc ctgcgcccgg gatccccgta ctcgggacac taacctggag gtaaccgccg
     1921 atgacggcat cgtgaccgtc accggaacga ctcagtcgcc ggtggtggtg ggggcggttc
     1981 ccatggtggc ccgtcaggtg gagggggtga gggacgtcag ggcggaggta aggctcctcc
     2041 gggagggtcg tggcggccct gcttgactcg gcggatccaa ccctgaagcc ggctccctat
     2101 cagccctcgg cttcccggcg gcggaaaacg aagagtggct gagagcggct caatgggagg
     2161 cgctgtatcc ttgccgctcc acgaactgac cgagggtcca gtaggcatga cccccatagg
     2221 ctaacgggtt ggccttggtc aggtcgatct caccctgcgc gtccagaatg gactcgtcca
     2281 cctgcaccgc gacgaccttc cccaggaaca gatcgtgcga gccgaggtgg attgtctgca
     2341 ccacctggca ctcaatattc agcgggcatt ccttgatcac ggctgttcgg atcacctgcg
     2401 tgggcacggg cgtcaggccc atcgcggcaa acttgtcttc cgagtgcccg gacacggtcc
     2461 cgcaccaatc caccacgcgg accatctccg ccgtggggac gttgacggcg aactcgccgg
     2521 actgcttcac cagcccgtgg gagtaccgcg acgggcggat gttgatgccg atgatcgggg
     2581 gctcggagca gagggtcccc atccacgcca gggtgatgat gttcggcttc tccgccgggc
     2641 cactggtgat caggcttgga ggaagcgggt aaaggtacgt cccgggcttc ttgctgacct
     2701 tcgccatggc tgaatcatcc ttccgcgaga tggtcgatcc aaccgtgcca gtcactcatc
     2761 tccaggggag tagaacgcac gccgctggga gaagtcctgg gtgtcgagag gatctgtggc
     2821 aggctcgtcc cgcttgaggc tctcgtaaat ctcctgcgcc aggggggcga cggcggcctg
     2881 aactgccggg gtgagacctt cggcgagggt ggcggtgtcg gccgcctcga ccgcgatgac
     2941 caccgcctcg tggggcatgg gaatgcccat tcgctcggcg aggcggaagg ccgtgccgat
     3001 gctcacctca tgcgcgttca ccaagcgtgc gcagcccgag acatcatcca gggtcagccg
     3061 gcacacgcgg ccgggccggg catcggggag caggatgcag tcgaggatga ccgcccgcgt
     3121 gtagccccgc aggagatcga tcagctcgaa tcccgcccgc gtgctggcca ggacgtccac
     3181 cccgcgtatc ggcgccaccg ccaggaggcg cttgagctcg gccgctacag ccagacctac
     3241 accgtcgtcg gagagaacgg gattccccag tcccaggacg acggtgcgtc cgccggcggc
     3301 catgaggatt cacggggtgc gccgagctcg ctgccagccg tccaggatct ggcgagcgcg
     3361 ctcaacatag ccggggagtg cctgatccac cgtaaggctc aggccttctc ggatctgata
     3421 ggggtcctcc acctccatgg cgagaacgca caggtcggtg gggaccggaa tggccagaca
     3481 gtcggccagc cgaagaacct ccggaagtcc ggcatagtgg ggagaaggtc caatcgtccc
     3541 gtggaagtca gcctgcgaga attcgagcac ggtccctgcc ggatggcgtc ccgtcaggat
     3601 cgcgtccagc agaagggcgt gtctccgtcc ctccagaaga tcgaggaggc ccaggccgga
     3661 ctcggcggcc tccacgacgt cgacctgcga ggaacagtgt gggcgaaggc ggcgggcggc
     3721 aatgaggccc actgcgtcgt ctccaaggat gtcgttgccg aggccgagga ccaggatacg
     3781 cggctgctgc tgggtcaccg ccggatttcc tgcaggacct gcccagaggc atcgcggatg
     3841 gagacaatca gcggcatctc gccgggaagc gagtgggtgg cgcagccatg gcacggatca
     3901 taggcccgga atgccatctc gatcatgttc aggaggccgt catccacctt gcccgcgtgg
     3961 atgagcccct tggcgctctt ctcgatgccc atggccatcc gcgccgagtt cccctgcgtg
     4021 gccacgatca gattggcctt cttgatcagt ccctgtt
//
LOCUS       DBNGZC010000029         4168 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_589244, whole genome shotgun sequence.
ACCESSION   DBNGZC010000029 DBNGZC010000000
VERSION     DBNGZC010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_589244"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /gene="atpD"
                     /locus_tag="AC16G0_00490"
     CDS             <1..871
                     /gene="atpD"
                     /locus_tag="AC16G0_00490"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR5098126.1"
                     /translation="VFSGVGERTREGNDLWLEMKESGVISKTALVYGQMTEPPGARLR
                     VGLTGLTAAEYFRDAGQDVLLFLDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMG
                     QLQERITSTKKGSITSVQAIYVPADDITDPAPANAFAHLDATTVLSRQISELGIYPAV
                     DPLASTSRILDPRFLGEEHYQTARSVQKVLQRYKDLQDIIAILGMDELSDDDKLVVSR
                     ARKIQRFLSQPFFVAEAFTGQPGRYVPVKETIRGFRGLVEGKGDDLPEQAFYMVGTID
                     EAREKAEKLKAVK"
     gene            907..1335
                     /locus_tag="AC16G0_00495"
     CDS             907..1335
                     /locus_tag="AC16G0_00495"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HIR5098127.1"
                     /translation="MAELGRTSFQLEVVTPDRLVVSDEVTELVAPGAEGYFGVLPGHI
                     PFITTLSIVELTYWRGRDERHLAVTWGYVEVLGEKVIVLAETALRAEEIDAERARKAR
                     QRAEGRLKEWTDGSGAIDHVRAEAALRRALTRLEVATKGR"
     gene            complement(1566..3050)
                     /locus_tag="AC16G0_00500"
     CDS             complement(1566..3050)
                     /locus_tag="AC16G0_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098128.1"
                     /translation="MDLHRIQDDLEQFARALCQEEYLIRAGLKDESRAAVIRERFSSL
                     GTRSAFEEARAEALAVAEPDRARQLRHLVEFLGTNCIEYQVRILIDRLATAEAGQTIT
                     VDGERLPLHAAEVRIRNEVDRARRARLESARLAAISEMSGLRREILQRAHEEAERLGF
                     AGYTAFCRELSGVDFAALRVLSQPILTRTLVMYQELIAWYLKRWVGVELRAARRHDLA
                     RLFRASDLDSSLPPGNLREAAEGSLHKMRIDPDAAGRIRVDDDPRPKKVPRAFVATIR
                     IPDEVILVVRPSGGVDDYQAFLHELGHALHLANTDPGLDVLDRRLGDPSVTEAFAFLL
                     DGLLRERGWLRRFVGLSQPHEVLRFTALHKLWYLRRYTAKLEYELVLHAGGSTSQAAD
                     LYRDLLSGATLVDWPRELYLADVDPFFYAARYLRAWIFEAQLRDLLRDRFDEEWYRND
                     RTGPFLLELWRQGQRYNLEELSGQLGLGPFTLDPLLNQITADLA"
     gene            complement(3203..3577)
                     /locus_tag="AC16G0_00505"
     CDS             complement(3203..3577)
                     /locus_tag="AC16G0_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR5098129.1"
                     /translation="MPIYEYLCQDCRRRVSVLVRSISRPGTPTCPRCGGRRLERLMSR
                     FSRVRSEDARLDSLADPSSLGDLDENDPKSVARWAKKMGKEMGEDLGEDFDQVMEEAM
                     AEEGAGKEGGETAAGDDLGDDL"
     gene            complement(3609..>4168)
                     /locus_tag="AC16G0_00510"
     CDS             complement(3609..>4168)
                     /locus_tag="AC16G0_00510"
                     /inference="COORDINATES: protein motif:HMM:NF052219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HIR5098130.1"
                     /translation="MYAHTTRQGSRTRTVDPYRLLPSPRGWDLIAYCHDRRRVLKIPL
                     TRLRDASVTRRRFPPLPARLLERHLHRSQAASLGIQWVRILCRPPLVQALRRHPPVGS
                     LMWEDGPEGSVIFTVGTRRMEDFIPWLLACGDAVEVLEPVSLRQQISKIARTIADRHS
                     AGSLASGGVTLSGIRGSRPSPEPTT"
BASE COUNT          756 a         1333 c         1411 g          668 t
ORIGIN      
        1 ggtcttctcg ggcgtgggcg agcggacacg cgaggggaac gatctctggc tggagatgaa
       61 ggaatcgggc gtcatcagca agacggccct cgtgtacggt cagatgaccg agccgcccgg
      121 cgcacggctg cgcgtgggac tcacaggcct caccgcggcc gagtacttcc gcgatgccgg
      181 ccaggacgtt ctcctgttcc tcgacaacat cttccggttc acccaggccg gatccgaggt
      241 ctcggccctc ttgggtcgga tgccctcggc ggtgggatac cagccgaccc tggccacgga
      301 gatgggccaa ctccaggagc gaatcacctc caccaagaaa ggctccatca cctccgtcca
      361 ggcgatctac gtgccggccg acgacatcac cgacccggcg ccggccaacg ccttcgccca
      421 cctggacgcg accacggtgc tctcccggca gatctccgag ctggggatct acccggcggt
      481 ggacccactg gcctccacct cccgcatcct ggatccgcga ttcctgggtg aggagcatta
      541 ccaaaccgcc cggtccgtcc agaaagttct ccagcgctac aaggacctcc aggacatcat
      601 cgccattctg ggcatggacg aactgtcgga cgatgacaag ctggtggtgt cgcgcgccag
      661 gaagatccag cgctttttgt cgcagccctt cttcgtcgcc gaggcgttca cgggccagcc
      721 cggccggtac gtcccggtca aggagacgat tcggggcttc cggggactgg tggagggcaa
      781 gggcgacgac ctgccggagc aggccttcta catggtcggg acgatcgacg aggcccgcga
      841 gaaggcggag aagctcaagg cggtgaagta atctccggga caagtcaggc cgtcagcgag
      901 tgtgccatgg cagagcttgg acgaacgagc ttccaattgg aagtggtgac gccggatcgg
      961 ctcgtggtga gcgacgaggt cacggagcta gtggcgcccg gagccgaggg ctacttcggc
     1021 gtcctgccgg ggcatatccc cttcatcacg acgctctcca tcgtggagct gacctactgg
     1081 aggggtcggg acgagcgaca cctggccgtc acctggggct atgttgaggt cctgggagag
     1141 aaggtcatcg tcctcgccga gaccgctctg cgcgccgagg agatcgatgc ggagcgggca
     1201 cggaaggcgc gccagcgggc ggaggggcgc ctgaaggaat ggacggacgg ctccggggcc
     1261 atcgaccacg tccgcgccga ggccgcgctg cgacgtgcgc tcacacggct ggaggtggcg
     1321 accaagggcc gctgagaaac taacccctca ttcaccacag attgcgcaga tctccccgaa
     1381 gagtcctgtc cgaaattcct gtctcccgaa tctgtccgtc atgtttatga cggtgcccgt
     1441 ccgcccagtc gaggtgcgcg gaagagcgca tgcgtccgca ggaaacccgc gcattcggtt
     1501 cgggaccatt ccgacggaat ctgcgcaatc tgcgtaatct gcggataaac ttgtgccctc
     1561 ggaggtcagg ccaggtcggc ggtgatttgg ttgaggaggg ggtcgagggt gaaagggccg
     1621 agaccgagct gccccgataa ctcctccagg ttatagcgct gtccctggcg ccacaattcc
     1681 aggaggaaag ggccagtgcg atcgttcctg taccactcct catcgaagcg gtcgcgcagg
     1741 agatcgcgga gctgggcctc gaaaatccag gcgcggaggt atcgggcagc ataaaagaag
     1801 gggtccacat cggcgaggta cagctcgcgc ggccagtcca ccagggtggc gccggagagg
     1861 aggtcgcgat acagatcggc ggcctgcgag gtcgatcccc cggcgtgaag cacaagctcg
     1921 tattccagct tggcggtgta tcggcggaga taccagagct tgtgcagcgc ggtgaacctc
     1981 agaacttcgt gaggctgcga gaggccgacg aagcggcgaa gccagccccg ctcccggagg
     2041 aggccgtcca gcaaaaaggc gaaggcctcg gtcaccgagg gatcgcccaa tcgccggtcg
     2101 aggacgtcca ggcccggatc ggtgttggca agatgaaggg cgtgtcccag ttcatggagg
     2161 aaggcctggt aatcgtccac gcccccggaa ggccggacca ccaggatgac ctcgtccggg
     2221 atccggatgg tcgccacgaa cgcgcgcggg accttcttcg gccgggggtc gtcatccact
     2281 cggatgcgac ccgcggcgtc ggggtcgatc cgcatcttgt ggagcgaacc ttcggcggcc
     2341 tcgcggagat tccccggggg caggctgctg tcgagatcag aggcgcggaa gaggcgggcc
     2401 agatcatgcc gccgggcggc ccgcagctcc acgccaaccc atcgcttcag ataccaggcg
     2461 ataagctcct ggtacattac caaggtacgg gtcaggatcg gctgggagag gacccggagg
     2521 gcagcgaaat cgactcccga cagctcccgg cagaaggccg tgtacccggc gaagccaagg
     2581 cgctccgcct cctcgtgggc gcgctgcaga atctcgcggc ggaggccgct catctcggag
     2641 atggcggcga ggcgggccga ctccagcctc gcccggcgcg cgcgatcgac ctcgttccgg
     2701 atgcggacct cggcggcatg aagcgggagc cgctccccgt cgaccgtgat cgtctggccc
     2761 gcctctgcgg tggccagacg atcgatcagg atgcgcactt ggtactcgat gcagttcgtt
     2821 ccgaggaact cgaccaggtg ccgaagctgt cgggctcggt ccggttccgc aaccgctagg
     2881 gcttcggccc gggcctcctc gaatgcggat cgggtgccga gggaggagaa gcgctcccgg
     2941 atcacggcag cccggctttc gtctttcaga cccgcccgga tcaggtattc ctcctgacag
     3001 agggcgcggg cgaactgttc gagatcgtcc tggatacgat gaaggtccat ggtggaaaga
     3061 cagccatcag ccgtcgacat ccggcggcca gctcgggact cggaacggaa cgggagaccg
     3121 gtgcgagaaa cccaccggtc cagcgttccg ctggacgctg atgggcgatg gctgaaggat
     3181 tgccttcgct cttagcggga tgctagagat catccccgag atcgtccccg gcagcggtct
     3241 caccgccctc cttgccagcg ccctcctcgg ccatggcttc ttccatcacc tggtcgaagt
     3301 cttccccgag gtcctcgccc atctcctttc ccatcttctt ggcccagcgc gccacgctct
     3361 tgggatcgtt ttcgtccagg tcaccgaggc tgctgggatc ggcgagggag tcgaggcggg
     3421 cgtcctcgga tcgcacccgg gagaatcggg acatcagacg ttccagtcgg cgaccgccgc
     3481 agcgagggca cgtgggggtg cccggacggc taatgcttcg caccaggacg cttacccgac
     3541 gtcggcagtc ctgacagaga tactcgtaaa tcggcacggc ccctcctcca tcgatgaggc
     3601 ccagacgcct atgtcgtcgg ttcgggagat gggcgactcc cgcgaattcc ggaaagggtc
     3661 actccccctg aggcgagcga ccccgcagaa tgacggtcgg cgatggtccg ggcgatctta
     3721 ctaatctgtt ggcgcaggct aaccggctcc agcacctcga ctgcgtcacc gcaggccagc
     3781 agccacggga tgaagtcctc catcctccgc gtaccgacgg tgaagatcac gctgccctcc
     3841 ggtccgtcct cccacatgag ggaccctacc ggcggatgcc gcctgagcgc ctgaaccagc
     3901 ggggggcggc agaggattcg cacccactgg attcccaggg aggccgcttg gctgcggtgc
     3961 aggtgtcgct ccaggagccg ggccgggagg ggaggaaacc ggcgtcgcgt caccgaggcg
     4021 tcccgaaggc gggtcagggg gatcttcaag acccgacggc gatcatgaca atacgcgatc
     4081 aggtcccacc ccctggggga gggcaggagc cggtaggggt ccacggtacg agttcgcgac
     4141 ccctgtcggg tcgtgtgagc atacatga
//
LOCUS       DBNGZC010000030         3875 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_589266, whole genome shotgun sequence.
ACCESSION   DBNGZC010000030 DBNGZC010000000
VERSION     DBNGZC010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_589266"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..213
                     /locus_tag="AC16G0_00515"
     CDS             85..213
                     /locus_tag="AC16G0_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098131.1"
                     /translation="MKISYVGGGGLVWDTDLLTDMALTAAAHEMPEAIKLYLPEFR"
     gene            233..577
                     /locus_tag="AC16G0_00520"
     CDS             233..577
                     /locus_tag="AC16G0_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR5098132.1"
                     /translation="MHAAGLRCPNDVVVDRRSALHGLRRPAAHDVALPVEDAGRRAAE
                     RLQEWIRRGNTSTDAEMLPCRLIVRESCGAPAEHDAQPGRRPRKAKNEGTLAEETAPH
                     CTATPARARGTP"
     gene            574..1830
                     /locus_tag="AC16G0_00525"
     CDS             574..1830
                     /locus_tag="AC16G0_00525"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098133.1"
                     /translation="MKRGALFATVLLVLGMVLLAGSSAQAQVTTLRFLWFTDGPDKPA
                     IEALVKRFNDTNKDIKIEFTIVPFAELNQLLVTQASGGQAPDLARVTEPYRFFQHTLD
                     LRPHLKNANFVKEFLDEPVKSVTGARGEFYGIPHDLSMNGPLVNVSLFKKAGVALPKG
                     DRVTWEEWMAAAAKVKKTTGVPFAAAVDRSGHRLDGFLQSYKGGFFTADGKDVRITSP
                     ETKKGVEAFVGFHKDGTMPLDIWAGGTGYVGANQQFVNGQLVYYLSGNWQVSQFQQGI
                     GDKFEWAAVQNGYNAQAGGMPGGKFLIAFKNTKAPDKLARLVEFLGSADAMSEYASKA
                     MFLPTRKDLVRKGVRYPARNEDMNVFIKGVGQLPQSAYVDNYNLHFGQVGNEVRDRVT
                     QVITGELTVDKALELAQTKAKELLKQ"
     gene            1929..2807
                     /locus_tag="AC16G0_00530"
     CDS             1929..2807
                     /locus_tag="AC16G0_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5098134.1"
                     /translation="MRTQRTSFRLTPYLFLTPNVAIFVVFIIAPAIYNFFLSLYRTSP
                     FRPPVFVGPENFLYLLQKDDLFWRAVRNILVFVVGDVTLITGLSVLVGVLLNQNVRFR
                     GFFRSAFFYPVLLSPVVVALVWQWILNSQFGVLNGILQGLGLARQPWLLRADWAMFWA
                     IFVHVWATLGFFAMIVLAGLQAIPAALYEAADIDGAGRWKTFRHVTVPMLMPTLMVVL
                     ILGMIRSFEVFDHIYVLTGGGPGTATLLIVQYIYRSAFQLDQFGLAAAASVVLFVVIF
                     TLTVAQYLLGRSRESV"
     gene            2889..3746
                     /locus_tag="AC16G0_00535"
     CDS             2889..3746
                     /locus_tag="AC16G0_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5098135.1"
                     /translation="MATSSKSMQYRGGRVDAGTIGAYGCLVLGTAVMFLPVLWIVLSS
                     FKYLNEIYRIPPTFLPEHWITSNYGGALERFPFLRYLTNSVVVTVVATAITLAINSMA
                     AFALSKYRFPGREAIFLVILGTLMIPLQVIMLPVYLVIAKLGMANSLLGIIIPPSATP
                     TGVFLLRQYMLTIPDELIEAARMDGAGEFRIYWQVVLPLTRPALAVLTVFSIMWRWND
                     FLWPLIVINQDSLFTLQLGLARFRGELVTDWHYVLAMTVLSIIPITLVFAVLQRYLVS
                     GIATTGLKG"
BASE COUNT          665 a         1262 c         1245 g          703 t
ORIGIN      
        1 atcggatggt gggaaggggc agaattgaac tgcctgttga tttcgcctcg aaagcgcgtt
       61 attgataagt cgttttacct atagatgaag atcagctacg ttgggggtgg cggcctcgtt
      121 tgggacacgg acctgctcac cgacatggcg ctcacagcgg ccgcgcacga gatgccggag
      181 gccatcaagc tgtacctgcc ggagtttcgc tgagccatgg ggggcgcgcg gcattcacgc
      241 cgccggcctc cggtgcccga atgacgtggt ggtggatcgg cgatccgccc ttcatggcct
      301 tcgccgcccc gccgctcacg acgtggcgct ccccgtcgag gatgccgggc gacgggccgc
      361 ggagcggctc caggagtgga ttcggcgagg gaacacgagc acagacgccg agatgctgcc
      421 ctgccggctg atcgtgcgcg agtcctgcgg cgcaccggcg gaacacgacg cccagccggg
      481 gcggcgtcct cggaaggcca agaacgaagg cacactcgcg gaagagaccg cgcctcactg
      541 cacggcgacg cccgcaaggg caagggggac cccatgaaga gaggtgcact gttcgcaacc
      601 gtcctgctcg tcctcgggat ggtcctgctc gccgggtcat ccgcccaggc gcaggtgacc
      661 acccttcgct tcctctggtt caccgacggg ccggacaagc ctgccatcga agcgctcgtc
      721 aagcgcttca acgacaccaa caaggacatc aagatcgagt tcacgatcgt gcccttcgcc
      781 gagctgaatc agctcctcgt gacgcaggcg tccggcgggc aggcgccgga tctggcgcgg
      841 gtcaccgagc cctaccgctt cttccagcac acgctggatc tgcggccgca tctcaagaat
      901 gcgaacttcg tgaaggagtt cctggacgag ccggtgaagt cggtgaccgg cgcgcggggc
      961 gagttctacg gcatcccgca cgacctgagc atgaatgggc ccttggtgaa cgtgagcctc
     1021 ttcaagaagg ccggcgtcgc gctgcccaag ggcgaccgcg tgacatggga ggagtggatg
     1081 gcggccgcgg ccaaggtgaa gaagacgacc ggcgtgccgt tcgcggcggc cgtggaccgg
     1141 agcggccacc gtctggacgg cttcctccag tcttacaagg gcggcttctt cacggccgac
     1201 gggaaggacg tccgcatcac cagccccgag acaaagaagg gcgtcgaggc gttcgtgggc
     1261 ttccacaagg acgggacgat gccgctggac atctgggcgg gcggcaccgg ctacgtcggc
     1321 gccaaccagc agttcgtaaa cggacagctc gtttactacc tgtcgggaaa ctggcaagtg
     1381 tcgcaattcc agcagggcat cggcgacaag ttcgagtggg cggccgtcca gaacgggtac
     1441 aacgcgcagg ccggcgggat gccgggcggg aagttcctga tcgccttcaa gaacacgaag
     1501 gccccggaca agctggcccg gctcgtggag ttcctgggca gcgccgacgc gatgtcggag
     1561 tatgccagca aggcgatgtt cctgccgacg cgaaaggacc tcgtccggaa gggggtcagg
     1621 tacccggctc ggaacgaaga catgaacgta ttcatcaagg gcgtggggca gctcccgcag
     1681 tcggcctacg tggacaacta caacctgcac ttcgggcaag tggggaacga ggtcagggac
     1741 cgggtgacgc aggtcatcac cggcgagctg accgtggaca aggcgttgga gctggctcag
     1801 accaaggcca aggagctgtt gaagcagtga cggcccgagc ggccattcgc cgttggccct
     1861 gatcctacat cccacaccgc aggcggccaa tgggcgaacg gctggtgggc gtccgaatga
     1921 ggcattcgat gcggacacaa cgaacgtcct ttcgcctcac gccgtacctc ttcctgacgc
     1981 cgaacgtggc gatcttcgtg gtcttcatca tcgcgcccgc catctacaac ttcttcctga
     2041 gcctgtaccg cacctcgccc ttccgccccc cggtcttcgt cgggcccgag aacttcctgt
     2101 accttctcca aaaggacgac ctcttctggc gggcggtgcg gaacatcctc gtgttcgtcg
     2161 tcggggatgt cacgctcatc accggcctgt ccgtgctggt cggcgtgctg ctgaaccaga
     2221 acgtccggtt tcgcgggttc ttccggagcg cgttcttcta cccggtgctc ctgtcgcccg
     2281 tggtcgtcgc cctcgtctgg cagtggatcc tgaacagcca gttcggcgtg ctgaacggga
     2341 tcctccaggg cctgggcctc gcgcggcagc cctggctgct ccgggcggac tgggccatgt
     2401 tctgggccat cttcgtccac gtgtgggcca cgctgggctt cttcgcgatg atcgtgctcg
     2461 ccgggctgca ggcgatcccc gccgccctgt acgaggcagc cgacatcgac ggcgccggcc
     2521 ggtggaagac cttccgccac gtgacggtgc ccatgctcat gccgaccttg atggtcgtcc
     2581 tgatcctcgg catgatccgc agcttcgagg tcttcgacca catctacgtg ctgaccggcg
     2641 gcggacccgg cacggcgacg cttctgatcg tccaatacat ctaccgctcg gcgttccagc
     2701 tggaccagtt cgggctcgcg gccgcggcgt cggtggtgct gttcgtcgtc atcttcacgc
     2761 tgacggtcgc ccagtacctg ctcggccgct cgcgggagtc ggtgtgagag aagcgctcag
     2821 cgattagcaa tcaacggtca gctcagtgca ctccacagct gatcactgca agctgatgag
     2881 aggaagacat ggcgacttcg agcaagtcga tgcaataccg gggcggacga gtggacgcag
     2941 gcaccatcgg cgcctacggc tgcctcgtgc tcgggacggc ggtgatgttc ctgccggtgc
     3001 tctggatcgt cctgtcgtct ttcaagtacc tgaacgagat ttatcggatc ccgcctacct
     3061 tcctgccgga acactggatc acgagcaact acgggggcgc ccttgagcgc ttccccttcc
     3121 tccggtacct gaccaacagc gtggtggtca cggtcgtcgc caccgccatc acgctcgcca
     3181 tcaactcgat ggcggccttc gccttgagca agtaccgctt tcccggccgg gaagccatct
     3241 tcctggtgat cctcgggacc ctcatgatcc cgctgcaggt catcatgctg ccggtgtacc
     3301 tggtcatcgc caagctcggt atggcgaact cgctcttggg catcatcatc ccgccatcgg
     3361 cgacgcccac cggcgtcttc ctcttgcgcc agtacatgct gaccatcccc gacgagctga
     3421 tcgaggcggc gcgcatggac ggcgccggcg agttccgcat ctattggcag gtggtcctgc
     3481 cgctgacgcg accggcgctg gcggtgctca ccgtgttctc catcatgtgg cgctggaacg
     3541 atttcctctg gcccctcatc gtgatcaatc aggatagcct gttcacgctt cagctcggcc
     3601 tggcacgatt ccggggggag ttggtcaccg actggcacta cgtcctcgct atgaccgtcc
     3661 tctccatcat ccccataacg ctggtcttcg ccgtgctcca gcgctacctc gtgagcggta
     3721 tcgcgaccac cggcctgaag ggctgacgag gaggatcagg tgtgcccgag acgcctcctc
     3781 tgtcctgaaa tcgagagcgc catcgccgtg gaggtgcagg catgaaaccg tggcaggccc
     3841 aacgctgggc attcgaaggc gaaggccgtg acctc
//
LOCUS       DBNGZC010000031         4198 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_882744, whole genome shotgun sequence.
ACCESSION   DBNGZC010000031 DBNGZC010000000
VERSION     DBNGZC010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_882744"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="AC16G0_00540"
     CDS             <1..321
                     /locus_tag="AC16G0_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5098136.1"
                     /translation="NRKGEVLSGGEQQMLTIARTLMGNPDLVLLDEPTEGLSPLMVTE
                     VMKIIRELKEKGETILLVEQNSTLALSVSQRAYILENGHIVYAGPADALSADREAKQR
                     YLGI"
     gene            complement(726..1403)
                     /locus_tag="AC16G0_00545"
     CDS             complement(726..1403)
                     /locus_tag="AC16G0_00545"
                     /inference="COORDINATES: protein motif:HMM:NF016874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HIR5098137.1"
                     /translation="MPTSTPKGRRQWPAKRPDRHRIYCAGPLFNRPEQEEMAGIAHTL
                     EGAGFSTFLPHKDGFVFEDVHREFLRGGYDDAEASRMIQRAIFCLDTYEVVSACDGLV
                     LNLNGRVPDEGAVAEAAMAWMANKSIVLYKSDSRSLIRGNDNPLVAGLGNFVKVSTLP
                     EIAYAFTQVFRARRAVSSPALPRLVRDAVEPGRRLSKTLADCQSPADIVPAIVAFARR
                     LPRFCAD"
     gene            complement(1434..2102)
                     /locus_tag="AC16G0_00550"
     CDS             complement(1434..2102)
                     /locus_tag="AC16G0_00550"
                     /inference="COORDINATES: protein motif:HMM:NF014269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR5098138.1"
                     /translation="MSVVIHIPIFPLPDVVLFPHTLLPLHIFEARYRQMVRDCLAGDK
                     RLAMALLKPGWENDYYGRPPIYPIAGAGEIIQHEELPDGRFNIILRGTMRIGILTELP
                     PDKPYRMARARPLPDGYSNEDPKGLADRVERLKVFYLRILAEVQKGQGEVAKIFSGIK
                     DPGIILDRIAGAAIPEAEVRQQVMEAVELPMRLLRVQEQLLGVLARISDRNAPAERAS
                     GRQN"
     gene            complement(2266..3057)
                     /locus_tag="AC16G0_00555"
     CDS             complement(2266..3057)
                     /locus_tag="AC16G0_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5098139.1"
                     /translation="MDLGLKVKVAIVAASSKGIGRAAALGLAAEGARVTMLARNESQL
                     RKAAEEIRDTCRAEVLAVPADVTRYEDIRRVVGSTVERWGAVNILVNNAGGPPVGNFD
                     DMDDARWQSAFELTLLSAVRFVREVRPHMSKAGGGAVINVQSTSVKIPLDNMILSNTI
                     RSGVMGLAKTLSLELAQDKIRVNTVLPGATLTDRLRHSMAAHAQKLGKTLDEVLRARE
                     ATIPLGHLGEPEDVANMIVFLASDRARYVTGVTIQVDGGLVRSIL"
     gene            3332..3841
                     /locus_tag="AC16G0_00560"
     CDS             3332..3841
                     /locus_tag="AC16G0_00560"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR5098140.1"
                     /translation="MTRFEQVMVAANRWVIFVMMAVMATLVFVNVICRYVFNFSIIWA
                     EEVSQYLMIWIAYLGAGLALREGRHVALELLQDSLPVALGRKLRMVVAGLVLAFLGAV
                     TVLGFQFAVFVWNQETPVLNISLGIPSLAIPIGSLLFSVHFLLMFRNYADRRYVPGYV
                     LEDLSGEKV"
     gene            3845..>4198
                     /locus_tag="AC16G0_00565"
     CDS             3845..>4198
                     /locus_tag="AC16G0_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR5098141.1"
                     /translation="MGITLVVTFLLGMAIGFPVAISMGGSGFLALLVDGKFSLLTVPQ
                     RFFNGINSFPLMAVPFFILAADLMSASGITMSLLRFTNNLVGHIRGGLGHVNVLTEMI
                     FSGISGSALADAAGPG"
BASE COUNT          737 a         1331 c         1306 g          824 t
ORIGIN      
        1 aaccgcaagg gcgaggtgct gagcggcggc gagcagcaga tgctcaccat cgcgcgcacg
       61 ctgatgggca acccggacct ggtgctgctg gacgagccga ccgagggcct ctcgccgctg
      121 atggtgacgg aggtcatgaa gatcattcgc gagctgaagg aaaaagggga gaccatcctg
      181 ctggtggagc agaattccac actggccctc agcgtctcgc agcgggctta catcctggaa
      241 aacggtcaca tcgtgtatgc tggaccggcc gacgccttga gcgccgaccg cgaggccaag
      301 cagcggtacc tcggcatctg agaggactca cgacctggag gccatcccca gaagcatcac
      361 ggttccacga acgttgaagc ttggttgatc ggacccggcc cctcctggca ggaatccttg
      421 gccgtcttcc tcgcacgctt cccgacgcac agaacctgcc actcgcatcc gtccttccct
      481 tgccctcatc gcggcgcttg atgggttgag agccagccca tctgaaccca acttctgcaa
      541 ttcaaccgca agaagcgcaa aggaacctcg tggattcccc gctgacgagg aaccactcgg
      601 atgaacggcc cggaagattt cgatcctgat gacgttctgg attgaggaga gctggccagt
      661 ctttgcgatc ttggcggtct tggtggtgaa attttccggc tgaatctgag gctggggcga
      721 gcggctcagt cggcgcagaa gcgggggagg cggcgggcga aagcgacgat agccgggacg
      781 atgtctgcgg gtgactggca gtcggccaag gtcttcgaga gacggcgacc gggctcgacc
      841 gcgtcccgca cgagtcgggg gagcgcggga ctcgaaaccg cgcgccgggc ccggaagacc
      901 tgggtaaagg cataggcgat ctccggcagg gtggagacct tcacgaagtt accaaggccc
      961 gccaccagcg gattgtcgtt tccccggatc aggctgcggc tgtcggactt gtagaggacg
     1021 atggatttgt ttgccatcca cgccatggcg gcctcggcta ccgcgccctc gtcgggaacc
     1081 cgtccgttca ggttcaggac caggccgtca caggcggaga cgacttcgta cgtgtccagg
     1141 cagaagatcg cgcgctggat catccggctt gcctcggcgt catcgtagcc tccccgcagg
     1201 aactcgcggt gaacgtcctc gaagacgaat ccgtccttgt gcgggaggaa ggtagagaaa
     1261 cccgcccctt ccagagtatg ggcgatcccc gccatctctt cctgctccgg gcgattgaac
     1321 agcggcccgg cacagtagat tcgatgacgg tccggccgct tggccggcca ctggcgccgt
     1381 cccttgggag tggatgtggg catgggctga tgggatgaga tcgtctgccg cggttagttc
     1441 tgacgtccgc tggctcgctc ggcaggcgcg ttacggtccg agatgcgtgc cagaactccc
     1501 aggagttgct cctgcacccg gagcagccgc atcgggagtt ccacggcctc catcacctgc
     1561 tggcggacct cggcttcggg gatggccgcg ccggcaatcc ggtccaggat gatgccggga
     1621 tctttgatac cactgaagat cttcgcaacc tcaccctgcc ctttttgcac ttcggccagg
     1681 atccgcagat aaaacacctt gaggcgttcc acccggtcgg ccagcccctt gggatcttcg
     1741 ttcgaatacc catccggcag cggccgggcg cgagccattc gatagggttt gtccggtggc
     1801 aattccgtca ggatgccgat acgcatcgtg ccacgcagga tgatgttgaa ccggccatcc
     1861 ggcaactcct cgtgctggat gatctctccc gcgcccgcga tcgggtaaat cgggggccgt
     1921 ccgtagtaat cattctccca gccgggcttg aggagcgcca tggccaggcg cttgtcgccc
     1981 gccagacaat cgcggaccat ttgcctgtat cttgcctcga agatgtggag cggcaggagc
     2041 gtgtgtggaa acaacaccac gtccggcagc gggaagatcg ggatgtggat gacgacgctc
     2101 atttggggca tcggtccagt agtctgtaag tgtgttttca ggcgttttac aataccacga
     2161 aaggcgggtg ctgggaagca ggagaaatgg gccatggcgg ccccgcggaa ccgatctctc
     2221 aatcggaccg gccgggacat cgtcgccatg tccacccaac tggggctaca ggatgctccg
     2281 caccaacccc ccgtccacct ggatcgtcac gccggtgacg taccgggccc ggtccgatgc
     2341 caggaacacg atcatgttgg ccacatcctc cggctccccg agatgcccca gggggatggt
     2401 ggcctctcgt gcgcgcagga cctcatcgag ggtcttcccc aatttctgcg catgcgcagc
     2461 catggagtgg cgcaggcggt ccgtcagggt ggcaccgggc aagacggtgt tcacccggat
     2521 cttgtcctgg gccagctcca gcgagagggt cttggccagg cccatgaccc cgctacgaat
     2581 cgtgttggac aggatcatgt tgtccagggg gatcttgacc gaggtggact gcacgttgat
     2641 gacggctccg cctcccgctt tcgacatgtg cggccgaacc tcgcgcacga atctgacagc
     2701 gctcaagagg gttagctcga acgccgactg ccaccgggca tcatccatat catcgaaatt
     2761 ccccaccggg ggtccgccgg cgttgttcac caggatattc acggctcccc atcgctcgac
     2821 cgtgctcccg accactcgtc ggatgtcctc atatctggtg acgtcggccg gcacggcgag
     2881 aacctccgcc cggcacgtgt cccggatctc ctccgcagcc ttcctgagtt gggattcgtt
     2941 ccgagccagc attgtcaccc tggccccctc ggcagcaaga cccagagccg cggccctccc
     3001 gatgcccttg ctcgacgcag ccacgatggc cactttcacc ttcagcccaa gatccatctc
     3061 gcgctctcct tcttgtcttc tgcttatcct gtccactgcc cggaggcccg ccggccggca
     3121 ctgcctaaga ggcggcgaga ttatactcga cggttggcca ttcgacaaaa gccaacccgt
     3181 acgagatgtt cgacctggga cgtgcaggga cgcaggcgaa agctcccaac gctcggagaa
     3241 cgtccgctgg aggataccgc tggaggatat ccccttgacg gcgaagtagg ctagagatac
     3301 tgatgggccc cgggaaagga ggtatccctg cgtgactcgc tttgagcagg ttatggtcgc
     3361 ggccaaccgg tgggtcatct tcgtcatgat ggcagtcatg gccaccctgg tgttcgtcaa
     3421 cgtgatctgc agatacgttt tcaacttctc catcatctgg gcggaggagg tctcacagta
     3481 cctgatgatc tggatcgcct acctgggggc tgggctggcc ctgcgcgagg ggcgtcatgt
     3541 ggccctcgag ttgctgcaag acagcctgcc cgttgctctt ggccggaagc tgaggatggt
     3601 ggttgcgggc ctcgtgctgg catttctcgg ggcggtgacg gttctcggct tccagttcgc
     3661 cgtctttgtc tggaaccagg agacccccgt cctgaatatc tccctcggga tcccatccct
     3721 ggcgattccc atcggttccc ttctcttcag cgtgcacttc ctgctcatgt tccggaacta
     3781 tgccgacagg cggtacgtgc ccggatacgt cctggaggat ctttcggggg agaaggtctg
     3841 agccatggga atcaccctgg ttgtgacctt tctcctcggc atggcgatcg ggtttccagt
     3901 cgccatcagc atgggcgggt cggggtttct ggccctcctg gtggacggca agttctccct
     3961 cctcaccgtt ccccagcggt tcttcaacgg gatcaactct ttccccttga tggcggtccc
     4021 cttcttcatt ctggcagccg acctcatgag cgccagcggc atcaccatgt ccctgctccg
     4081 cttcaccaac aacctggtcg gccacatccg gggcggcctc gggcacgtca acgtgctgac
     4141 ggagatgatc ttctccggga tcagcggctc ggcgctggcg gacgccgcgg gccccggg
//
LOCUS       DBNGZC010000032         2147 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_882760, whole genome shotgun sequence.
ACCESSION   DBNGZC010000032 DBNGZC010000000
VERSION     DBNGZC010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_882760"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="AC16G0_00570"
     CDS             complement(<1..294)
                     /locus_tag="AC16G0_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098142.1"
                     /translation="MRTYGELQLFTPGPVNVPARVLAAGARPMLHHRTAEFSRILSCL
                     IEKAQRLIGTHQDILPVHASGRGAMDGTITNLFSPGDEILCICNGRFGEMYAEI"
     gene            complement(511..1701)
                     /locus_tag="AC16G0_00575"
     CDS             complement(511..1701)
                     /locus_tag="AC16G0_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HIR5098143.1"
                     /translation="MHEKAKALLDVAKTIKGGQLYKWGTGGSFSSHALAGSLPVRNYG
                     TNIFPEHEQMSGEYVRTHFEHRDKACWACGLVHTKFTKVTEGPYTGYEGEEPELECMV
                     AWGPVIGNKDPGAAVMLTDVTDRLGLDVNEAGWVVAWAMECYEKGLLSRADLDGLELG
                     WGNPEAAKALLEKIARREGIGDLLAEGVKRAAERTGGDAVKMGVYVLKGATPRGHDHR
                     AVWSEMLDTCVSATGTIQSAARLTSPANFGQPPLSNPFSPWEVAGANARLEGWFVFLD
                     SLPICRFISIHPQLTMDCVNAITGRNLSLSQALLIGRRSVNQLRVFNFRHGLDPGLEA
                     PSPRYGSAPTDGPAQGKSIAPYFQWMKSFYFELMGWDPATGKPLPQTLKSLGLEKLIP
                     DLEA"
     gene            complement(1705..2052)
                     /locus_tag="AC16G0_00580"
     CDS             complement(1705..2052)
                     /locus_tag="AC16G0_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014750.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5098144.1"
                     /translation="MKSAGYDAVIIQGRAPAWVYLHIAEGRAELRDAAGLLGTDTWDL
                     EEAVRRDLGAGKGLSVFGVGPAGEHGVRFAVVAGDQGHICSKNGCGAVMGAKRLKAVA
                     VTRGRIPVPVFDK"
BASE COUNT          389 a          724 c          647 g          387 t
ORIGIN      
        1 tatctcggcg tacatctcac cgaatctccc gttgcagatg cagaggatct catcccccgg
       61 agagaagaga ttggtgatcg tcccatccat cgccccgcgg cccgacgcat gcaccggcaa
      121 tatgtcctgg tgggtcccga tgagccgctg cgccttctcg atgagacagg agaggatacg
      181 cgagaactcg gccgtccggt ggtgcagcat gggtcgggca cctgcggcta gtacccgagc
      241 ggggacgttg acgggtcccg gcgtgaacag ttgaagctcc ccgtatgttc tcatgcgcgt
      301 ccctttcccc gaacaggctg gtgaaaaaga ctcatgtact gaagtgcggt gtccgaagcc
      361 ctccggtcgc tcctgtctgc agtggtcgat gggcggcgag agaatcgcgc cacccaatgc
      421 agattgtcaa gagggaagac cgtcactgac ctgggatcag acctcgcatg gagccccctt
      481 caggcagcga gatggagact ccctcgacca tcaggcttcc aggtcgggga taagtttctc
      541 cagcccaagg gacttcaggg tctggggcag gggcttgccc gtcgccggat cccatcccat
      601 cagctcgaag tagaaactct tcatccattg gaaatagggt gcaatggact tcccctgggc
      661 gggcccgtcg gtaggcgcag agccgtagcg aggggagggg gcctcaaggc caggatcaag
      721 gccatgccgg aagttgaaga cccgcaattg atttacgctg cggcgcccga tcagcaacgc
      781 ctgggatagg ctgaggtttc ttccagtgat ggcgttcacg cagtccatcg tcaattgcgg
      841 atggatgctg atgaatcgac agatcggcag actgtcgagg aacacgaacc agccctcaag
      901 ccgggcgttt gcgcccgcga cctcccacgg ggagaaggga ttgctcagag gcggctggcc
      961 gaaattggcc ggggaggtca ggcgggcggc cgactggatt gtgccggtcg ccgacacgca
     1021 ggtgtccagc atttccgacc agacggcacg gtgatcgtgc cctctgggag ttgcgccctt
     1081 gaggacatac acccccatct tcaccgcatc tccgcccgtc cgctcagcag cccgctttac
     1141 gccttcggct agcaagtcgc caatgccctc gcgccgggca atcttctcga gcagcgcctt
     1201 ggccgcctca ggattgcccc accccagctc cagtccatcc agatcggcac ggctgaggag
     1261 gcccttctcg tagcactcca tggcccaggc gacgacccag cccgcttcat tcacatccag
     1321 ccccagccgg tcagtgacgt cggtaagcat cacggcggcc ccgggatcct tgttgccgat
     1381 gaccggcccc caggcaacca tgcactccag ctcgggctct tcgccttcat acccggtgta
     1441 gggcccttcg gtcaccttgg tgaacttcgt gtggaccagc ccgcaggccc agcaggcctt
     1501 atcgcgatgc tcgaaatggg tgcggacgta ctccccgctc atctgctcgt gttcggggaa
     1561 gatattggtc ccataattgc gaaccgggag gctgcccgcc agcgcgtggg acgagaagct
     1621 gccgcccgtc ccccacttgt agagttgacc gcccttgatg gtcttcgcca catccagcag
     1681 ggccttcgcc ttctcgtgca aaagttactt gtcgaacacg gggaccggga tgcgccctcg
     1741 ggtcacggcc accgccttga gccgcttggc gcccatgacc gccccgcagc cgttcttcga
     1801 gcagatatgg ccttggtcgc ccgccacgac cgcaaagcgt accccatgtt cgcccgccgg
     1861 cccaaccccg aagacgctca gtcccttgcc cgccccgagg tcccgccgga ccgcctcctc
     1921 cagatcccag gtgtccgtgc ccaggagacc ggcggcatcc cgtagctcgg ctctcccctc
     1981 cgcgatatgc agatagaccc aggccggcgc gcggccctgg atgatcaccg catcatatcc
     2041 ggccgacttg atgtaggccc cccagaagcc attggcttgg ctggagacgc cgcttgagca
     2101 gcatggggtt cgacccggcc gcgacgacgc gcaggccttc atcgaca
//
LOCUS       DBNGZC010000033         2060 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_491549, whole genome shotgun sequence.
ACCESSION   DBNGZC010000033 DBNGZC010000000
VERSION     DBNGZC010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_491549"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /locus_tag="AC16G0_00585"
     CDS             <1..1284
                     /locus_tag="AC16G0_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098145.1"
                     /translation="RRLKLRLLGGLVVWIATVYCGTSSRTGRGRGREGTGSYPELAPL
                     GIRKGATPALQSQVGRLTALLPSVELARDELRRQGPTLDEKAVHRMARQLGAEVLATR
                     TRDLQRYRDGQLPAGLEMAGHHIVAQMDGGRVRIRTQVEAKKRKGVKYRRKIRVEWRE
                     PKLLILYLSDRKGRMLRGTRPWIDGTMNGPDHLMELLAFHLHRLGAAQAKTVSFVSDG
                     APWIWNRLDWVVRRVGLDARRTERILDCCHAVHHISLALQALGLAEAERTATYRTLRH
                     QLRAGRSRDVVATLRTMAEGQPLDSEVWTEIEYLNKHEPHLRYDWFGYRGRPLGSGAV
                     ESAIRRVINLRLKGNGIYWKEENAEAMLVLRAAVLTGRWEETMERTQAAMARDRRRDW
                     QWAAPDIEAELNADAVIKPPMAQPEPSEQSEAIAA"
     gene            1399..>2060
                     /locus_tag="AC16G0_00590"
     CDS             1399..>2060
                     /locus_tag="AC16G0_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_277861803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HIR5098146.1"
                     /translation="MSLLDHPEAQALLDDATVTPDAVHDCTDRLTAFLQRDLPKFYRT
                     EQRENATLVIRGLISGLERKTCEPIAIEAGLPRKPVQFFVGAGKWDDEAVMGELRTHV
                     REELAEPDGVVVIDPSGFPKKGAESCGVARQWCGRLGKVDNCQIGVFLAYAAKAGYAP
                     LDRRLYLPEDWAADEARREKCHVPPEVEFQEKWRIAPDLLDRSLPGLPHGWITGDAEF
                     GRA"
BASE COUNT          342 a          662 c          731 g          325 t
ORIGIN      
        1 cggcggctga agctccgact cctgggcggt ctggtcgtct ggatcgccac ggtctactgc
       61 ggcacctcgt cccgcaccgg ccggggtcgg gggcgcgagg ggaccgggtc ctaccccgag
      121 ttggccccct tgggcatccg caagggcgcg acgccggccc tccagagtca ggtgggccgt
      181 ttgaccgccc tgctcccctc ggtcgaactg gcccgcgacg aactccgtcg ccaggggccg
      241 accctcgatg agaaggccgt ccaccgcatg gcccggcagt tgggtgccga ggtcctggcg
      301 acacggactc gcgatttgca gcgatatcgt gacggtcaac tgccggcagg tctggagatg
      361 gccggacacc acatcgtcgc ccagatggac gggggccggg tccggatccg gacccaggtc
      421 gaggccaaga aacgcaaggg agtgaagtac cgccggaaga tccgcgtcga gtggcgcgag
      481 ccgaagctgt tgatcctcta tctcagcgac cgcaaggggc ggatgctccg ggggacgcgc
      541 ccctggatcg acggcacgat gaacgggccg gatcacctga tggaactgct cgcattccat
      601 ctccatcgcc tgggagccgc acaggccaag acggtgtcgt tcgtctcgga cggagcgccc
      661 tggatctgga atcgcctgga ttgggtcgtc cggcgcgtgg ggctggacgc gaggcgcacc
      721 gagcggatcc tggattgctg ccacgcggtg catcacatca gcttggcatt gcaggcgctg
      781 ggcttggcgg aagcggagcg gacagcgacg tatcggacct tgcgccatca attgcgcgcc
      841 ggccggagcc gcgacgtggt ggcgaccctg cggacgatgg ccgagggtca gccgctcgac
      901 tcggaggtgt ggaccgagat cgagtacttg aacaagcacg agccccacct gcgttacgac
      961 tggttcggct accggggtcg tccgctgggc agtggtgcgg tcgagagcgc gatccgccgg
     1021 gtgatcaact tgcggctcaa aggcaacggg atctattgga aggaggagaa tgccgaggcg
     1081 atgctggtgc tgcgtgcggc ggttttgacg ggtcgatggg aggagacgat ggagcggacg
     1141 caggcggcga tggcgcggga tcgccgccgc gattggcaat gggcggcccc ggacatcgag
     1201 gcggagttga acgcggatgc ggtgatcaag ccaccgatgg cacaacctga accctcagag
     1261 cagtctgagg cgatcgccgc gtaacacttt tccggaatcc accctacttc tactgtaacg
     1321 gatttttcct gacaccctcg gttcattccg atatcatggc gtacgcggag gttacgctcg
     1381 ccgctcggag gccccgcgat gtcgctcctg gatcaccccg aggctcaggc cctcttggac
     1441 gacgccaccg tcacccccga cgccgtccac gactgcaccg accgcctgac cgccttcctc
     1501 cagcgggacc tgccgaagtt ctaccgcacc gagcagcgtg agaacgccac cctggtcatc
     1561 cgcggactga tcagcggcct ggagcgcaag acctgcgagc cgatcgccat cgaggccggg
     1621 ctcccccgca agccggtcca gttcttcgtc ggcgccggca agtgggacga cgaggccgtg
     1681 atgggagagc tgcggaccca cgtgcgcgag gagttggccg agcccgacgg cgtcgtggtc
     1741 attgacccca gtggcttccc caagaagggg gccgagtcct gcggggtggc acgccagtgg
     1801 tgcggccggc tcggcaaggt cgacaactgc cagatcggcg tcttcctggc ctatgctgcg
     1861 aaggccggtt atgcccccct ggaccggcgt ctgtacctgc ctgaggactg ggcggccgac
     1921 gaggcgcggc gcgagaagtg ccacgtcccg ccggaggtcg aatttcagga gaagtggcgg
     1981 atcgcgccgg acctcctgga ccggagcctg cccgggttgc ctcacggctg gatcacaggc
     2041 gacgccgagt ttggccgggc
//
LOCUS       DBNGZC010000034         2490 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_980848, whole genome shotgun sequence.
ACCESSION   DBNGZC010000034 DBNGZC010000000
VERSION     DBNGZC010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_980848"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1236)
                     /locus_tag="AC16G0_00595"
     CDS             complement(<1..1236)
                     /locus_tag="AC16G0_00595"
                     /inference="COORDINATES: protein motif:HMM:NF017197.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HIR5098147.1"
                     /translation="MLPIRDFLTRYWKKITIWTAALLAVFTLVGFFVIPPILKSILSK
                     QLTTALHRDVSIREVRVNPFALSLALRGLTVKERSGPETFASFEELYVNLESYSVLRL
                     AAIVREVRLTKPFARIVRNPDQSYNFSDLLEGSAPPKQAKQEPAKPPRFSINNIRSID
                     GGADFLDQVTQTKHTVREVNVGVPFISSIPSYVQTFVQPGISANVNGTRYAIQGKTKP
                     FADSRETTLDVNIADLDLPYYLAYVPQEFLTFAMPSGRLDAKLVIVFLQQKSQQSLSV
                     KGDVGFREIAIDEKKGGPVVKIPSLGISIASAEPLAQKVHLARVALESPELTVRREKT
                     GTTNLETLLPKPPPAKPAAEKTPTPAPQGGGPILDVDEISIAGAKVFVSDLLPRLPFK
                     TTLAPIDIKVAQLSNRQGTT"
     gene            complement(1423..>2490)
                     /gene="hutU"
                     /locus_tag="AC16G0_00600"
     CDS             complement(1423..>2490)
                     /gene="hutU"
                     /locus_tag="AC16G0_00600"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013082675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HIR5098148.1"
                     /translation="KTGYCEVMTTNLDEASALALGSKRAGIPKSIGLVGNAAEVHPEI
                     LKRGILPDLVTDQTSAHDSLNGYVTTGLSLEAARELRQKDPEQYIHRALDSMAVHVQA
                     MLEFQRRGAIVFDYGNNIRAQALSRGVKNAFDFPGFVPAFIRPLFCTGRGPFRWAVLS
                     GDPADIAKIDDVILREFKDNDSLVRWITMAREKVHFQGLPARICWLGYGERDRLGVLV
                     NRMVKSGDLKAPVVFGRDHLDAGSVASPNRETEAMRDGSDAIADWPILNALLNTSAGA
                     SWISVHHGGGVGIGYSIHAGMVVVADGTADTERRLQRVLTTDPGTGIMRHADAGYPEA
                     IAAAKEKGVKIPGLEAYPDHG"
BASE COUNT          444 a          739 c          841 g          466 t
ORIGIN      
        1 cgtggtaccc tggcggttgc tgagctgtgc caccttgatg tcgatggggg ccagggtagt
       61 cttgaagggc aggcggggga gaagatcgga cacaaagacc ttggccccgg cgatgctgat
      121 ctcatccacg tccagaatag gaccgccccc ttgaggagca ggggtgggtg tcttttccgc
      181 cgctggcttg gcaggcggcg gcttggggag cagggtttcc agattcgtcg tacccgtctt
      241 ctcccgacgg accgtcagct cgggcgactc cagggccact ctggcgaggt ggaccttctg
      301 ggccaaaggc tcggccgagg cgatcgagat gccgagggag ggaatcttga caaccggccc
      361 gcccttcttt tcgtcaatcg caatctcgcg aaagcccaca tcccccttca cgctgaggga
      421 ctgctgtgac ttttgctgca agaacacgat gacgagcttg gcatccagac gtcccgatgg
      481 catggcaaag gtgaggaatt cctgcgggac gtaggcaaga taatagggca ggtcgaggtc
      541 cgcgatgttg acatccaggg tcgtctcacg ggagtcggcg aagggcttgg tctttccctg
      601 gatggcgtac ctggtcccgt tcacgtttgc cgaaattccc ggctggacga aggtttggac
      661 gtaggatggg atactcgaaa tgaaggggac gccgacgttg acctctcgga cggtatgctt
      721 cgtctgcgtc acctggtcca gaaagtctgc gcccccatcg atgctgcgga tgttgttgat
      781 cgagaagcgg ggcggcttgg ccggctcctg cttggcctgc ttcggaggtg cgctgccttc
      841 cagcaggtcg gagaaattgt aggattggtc cggattcctg acgattcggg cgaagggctt
      901 ggtcagacgt acctctcgga cgatggctgc caaccggagc actgagtaac tctcgaggtt
      961 cacatacagc tcctcgaagg acgcgaaggt ctccggtccg gagcgctcct tcaccgtcag
     1021 gcctcgcagc gcgagcgaaa gcgcgaaggg attcacgcgt acctcgcgaa tactcacatc
     1081 ccggtgaagg gccgtggtca gttgcttgct caagatggac ttgaggatcg ggggaatgac
     1141 aaagaagccg accagcgtga agaccgccag gagggctgcc gtccagatgg tgatcttctt
     1201 ccaatagcgt gtgagaaagt ctcgaatcgg gagcatgagg cacctccggc tcagaaagca
     1261 gacgcatccg tgcgacccgt agggggtcgt ctacaggatg aagaaggatc cctctccacc
     1321 gacccgtggc cagcctgtat ggaagtggcc atccctcgtc cgcaccatgc ctgcggttgt
     1381 ccgcgaagcg tctggaaagg tgtggaatgg atcggctcta gctcacccgt ggtccgggta
     1441 ggcctcgagt cccggaatct tgaccccttt ctctttggcc gcggcgatcg cctcgggata
     1501 gccggcgtcg gcatgccgca tgatgccggt gcccgggtcg gtggtgagaa cgcgctggag
     1561 tcgccgctcg gtatcggccg tgccgtccgc cacgaccacc atgccggcat ggatggaata
     1621 gccgatgccg acgccgccgc cgtggtgcac ggagatccag gacgcccccg cggaggtgtt
     1681 gagcagggca ttcaggatcg gccaatcggc gatggcatcg ctgccgtccc gcatggcctc
     1741 ggtctcgcgg ttgggcgagg cgaccgagcc ggcgtccagg tggtcgcgac cgaacaccac
     1801 gggggccttc agatcgcccg acttcaccat ccggttgacc aggacgccca atcggtcccg
     1861 ctcgccatag cccagccagc agatgcgggc gggaagcccc tggaagtgca ccttctcgcg
     1921 cgccatcgtg atccagcgga ccagggagtc gttgtccttg aactcccgca ggatcacatc
     1981 gtcgatcttg gcgatgtccg ccggatcacc ggacaggacg gcccagcgga aggggccgcg
     2041 tcctgtgcag aacagcgggc ggatgaaggc gggaacgaaa ccggggaagt cgaaggcgtt
     2101 cttcacgccg cggctcaggg cctgggcgcg gatgttgttg ccgtagtcga acacgatggc
     2161 gcccctgcgc tggaactcca gcatggcctg gacgtgcacg gccatggagt ccaaggcccg
     2221 gtggatgtat tgctccggat ctttctgccg cagctcgcgg gctgcctcca ggctcaaccc
     2281 ggtggtaacg tagccattca gggagtcgtg ggccgaggtc tggtcggtca cgaggtcggg
     2341 gaggatgccg cgcttcagga tctcgggatg gacctcggcg gcgttcccca ccaatccgat
     2401 ggacttgggg atgcccgcgc gcttcgagcc gagcgccaga gccgaggctt cgtccaggtt
     2461 cgtcgtcatg acctcgcagt aaccggtctt
//
LOCUS       DBNGZC010000035         3129 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1176617, whole genome shotgun sequence.
ACCESSION   DBNGZC010000035 DBNGZC010000000
VERSION     DBNGZC010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1176617"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..673
                     /locus_tag="AC16G0_00605"
     CDS             179..673
                     /locus_tag="AC16G0_00605"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5098149.1"
                     /translation="MNPYAKYLENRDPLTVAAATPAKIVSLIRGLTPRQMAKRPAPGK
                     WSIQEIISHLADTEMVMCCRARWIAFEDRPALVPVDQEKWAAGRTREKEPLAETLERF
                     RLLRRSQIRLFRNAPRQDFRRTGFHPERGVVTLKVQLETLAGHDLNHLAQIRRLAALH
                     RARQ"
     gene            complement(834..1184)
                     /locus_tag="AC16G0_00610"
     CDS             complement(834..1184)
                     /locus_tag="AC16G0_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HIR5098150.1"
                     /translation="MASVELYYRDGCPSCAEAKEFLSQHRIEYAAHDVVRDPLNAHRT
                     MTLVGPCRKFFVKAGDEMFLWDRDQEEVPNEALRRHFVHTDGFIRIPFLIVDDTAVRG
                     FLPEIYSELLATPG"
     gene            complement(1228..2139)
                     /locus_tag="AC16G0_00615"
     CDS             complement(1228..2139)
                     /locus_tag="AC16G0_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIR5098151.1"
                     /translation="MQVAFQQVDVFTDRPFAGNPLCVVPEATGLSSEQMQAIAREMNL
                     SETTFVLPPTDPQATYWMRIFTPAKEIPFAGHPSIGTAHVMARSGRFRLGEPVTRVFQ
                     QVGIGTLPLDIEVAGGTPGRVIMTQGTLRFGPVIRDLDPVANALGLDPMILARAKLLI
                     QVVSTGLEHLMVPLPDLETVRALRPNLAALDVVLRKCSALGCFVFTLATGSSDALAHA
                     RMFAPGAGIIEDPATGSAAGPLGAYLAAHGVLPRERSSFVIEQGIEMGRPSRIWVEVG
                     RDGSGAPVAIRVGGNSVPVIAGTIQVS"
     gene            complement(2179..>3129)
                     /locus_tag="AC16G0_00620"
     CDS             complement(2179..>3129)
                     /locus_tag="AC16G0_00620"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HIR5098152.1"
                     /translation="DAEKAATKGIIMTEPTALQTLQDRFAIPGVVRFEMGERGLIRAN
                     IRTLRAEAQVWLHGAHVTHYRPGGDRPVLFTSERSRFEPGAPIRGGVPIVFPWFGPKA
                     GNFSAPMHGFARLEEWGVESVTQAPDGSVTMTLGMESGAKPHPHWPHAFVLRYRVVIG
                     ERLDLGLEIHNRAEAAITFEEALHTYLAVADVRQVSVAGLTGSRYIDKTDGMRRKVEE
                     SALVHITGETDRVYLETKATCILDDPVMGRRLSVEKSGSEATVVWNPWIAKAKAMPDF
                     GDDEWQRMLCIESGNVADHAVTLEPGQRHELRVSIRSLWS"
BASE COUNT          579 a         1032 c          979 g          539 t
ORIGIN      
        1 tcgacctttc ggtcaagagg ctcatcctcc cggggagcca gggttacctc tggcgccgtg
       61 aaggcgttcc tgcttgcggc acgtcagaat tcctgagaaa gtagcacgct acgtgtctcg
      121 ggatccgttc acagactcga ttggcgccgg aacaccattg aacggaaggg aggaaatcat
      181 gaacccctac gcaaagtatt tggagaaccg ggatccgctc acagtcgcgg ccgccactcc
      241 ggcaaagatc gtctcgctga ttcgcgggct cacccccagg cagatggcga aacgcccagc
      301 gccgggcaag tggtcgatcc aggagatcat cagccacctg gcggacaccg aaatggtcat
      361 gtgctgccgg gcgcgctgga tcgccttcga ggatcgcccc gcgctcgtcc ccgtcgacca
      421 ggagaagtgg gcagcaggcc ggacgcggga gaaagaaccg ctggcggaaa ccctcgagcg
      481 ctttcggctt ctgcggagat cccagatccg gctgtttcgg aatgccccca ggcaggactt
      541 caggcggacc ggcttccacc cggagcgggg tgtcgtcacc ctgaaggtgc aactggaaac
      601 actcgcaggt cacgatctga accatctggc gcagattcgg cgcctggccg cactgcaccg
      661 ggcgaggcag tgatttccgt ctggccgcga gtttccccgc atattgtggc ccgttcgaag
      721 cccaccagca agaatggttc acgcggtctt gccggggcgg gagccccacc cggtcggatc
      781 cgggggagcg ggggtgattg cagtgtgggc tgctgaagga cctctcgcct ggatcaaccg
      841 ggagtcgcga gaagctcgga gtagatttcg gggaggaagc cgcggacggc ggtgtcatcg
      901 acgatcagga acgggatgcg gatgaacccg tccgtgtgca cgaagtggcg gcgcagggcc
      961 tcgttcggga cctcttcctg atcgcgatcc cagagaaaca tctcatcacc cgccttgacg
     1021 aagaacttcc ggcacgggcc gacgagggtc atcgtcctgt gggcattcag cggatcccgg
     1081 accacatcgt gcgccgcata ctcgatccga tgctgcgaga gaaattcctt ggcctcggcg
     1141 caggagggac atccgtctcg ataatagagt tccacgctgg ccatgagtcc ctcccgtcga
     1201 accccggtgt gggcgttcga ccggtcttca cgacacctga atcgtgccgg cgataaccgg
     1261 gacgctattg ccccccaccc ggatcgcgac tggtgcgccg cttccgtcgc gtcccacctc
     1321 gacccagatg cggctgggcc gccccatctc gatcccctgt tcgatgacga agctcgaacg
     1381 ttcgcgcggc agcacgccgt gggcggccag gtacgctcca agaggacccg ccgccgatcc
     1441 ggtggcgggg tcctcgatga tgcccgcacc cggcgcaaac atgcgagcgt gggccaaggc
     1501 atcactcgat cccgtcgcaa gcgtgaacac gaagcagccg agcgcgctgc acttccgcag
     1561 gacgacgtcc agggcggcca ggttgggccg caatgccctg accgtctcca ggtcgggcaa
     1621 cgggaccatg agatgctcga gtcccgtcga gaccacctgg atcaggagct tcgctcgcgc
     1681 cagaatcatg ggatccagcc ccagggcatt cgccacgggg tccaaatctc ggatgaccgg
     1741 accgaaccgc agcgtcccct gggtcatgat gacgcggccg ggggtccctc ctgcaacctc
     1801 gatgtccagc ggcagcgtcc cgatgcctac ctgctggaag acgcgcgtga ccggctcccc
     1861 caggcgaaat cttccggagc gagccatgac atgggccgtc ccgatgctcg gatggccggc
     1921 aaaggggatc tccttggcgg gcgtgaagat ccgcatccag taggtcgcct gcggatccgt
     1981 gggcggaagg acgaaggtcg tctcggacag gttcatctcc cgcgcgatcg cctgcatctg
     2041 ctcgctggag aggcccgtgg cctcgggaac cacacacagc ggattccccg caaatggtcg
     2101 gtcggtgaac acgtccacct gctggaaggc tacctgcata tcccgcctct ccgttggatc
     2161 accattgcca tcccagaatc agctccacag actgcgaatc gagacccgca attcgtggcg
     2221 ctggccgggt tcaagcgtca cggcatggtc cgccacgttg ccgctctcga tgcagagcat
     2281 ccgctgccac tcgtcatccc cgaagtcggg catggccttg gccttggcga tccacggatt
     2341 ccagaccaca gtggcctcgg agcccgattt ctccaccgag aggcgccggc ccatcacggg
     2401 atcgtccagg atgcaggtgg ccttcgtctc gagatagact cgatccgtct cgccggtaat
     2461 gtggacgagg gcggactcct ccacctttcg tctcataccg tcggtcttgt cgatatagcg
     2521 ggaacccgta agcccggcca cggatacttg ccgaacatcg gccacggcaa gataggtgtg
     2581 cagagcttcc tcgaaggtga tggctgcctc cgcccggttg tggatttcga ggccgaggtc
     2641 cagcctctcc ccgatgacga cgcgatagcg gagcacgaag gcgtgaggcc aatgcgggtg
     2701 tggcttggcg ccagattcca taccaagagt catggtcacg gagccgtcgg gagcttgagt
     2761 gaccgactcc acaccccact cctcaagtcg tgcaaacccg tgcatcggcg cggaaaaatt
     2821 gcctgccttc ggcccgaacc aggggaacac gatcggcacc ccgccccgga tgggcgcacc
     2881 cggctcgaag cggctccgct cgctcgtgaa cagcacggga cgatcccccc cgggccggta
     2941 gtgagtcacg tgtgccccgt gcagccacac ctgtgcctcg gcccgaagcg tgcggatgtt
     3001 cgcgcgaatc agcccacgtt cccccatctc gaaccgcaca accccgggaa tcgcgaatcg
     3061 atcctgcaat gtctgaagcg cagtcggttc tgtcatgatt atgcctttgg ttgcggcttt
     3121 ctctgcgtc
//
LOCUS       DBNGZC010000036         4028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1274583, whole genome shotgun sequence.
ACCESSION   DBNGZC010000036 DBNGZC010000000
VERSION     DBNGZC010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1274583"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(260..844)
                     /locus_tag="AC16G0_00625"
     CDS             complement(260..844)
                     /locus_tag="AC16G0_00625"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018989890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HIR5098153.1"
                     /translation="MRLVVGISGSTGSIYGIRLLEVLRDIPDLESHLIISGPGKRTIL
                     EETDYSIKDVERLATHVYDNRDIGAALASGSFRTAGMVIAPCSVKTVSALANCHADTL
                     ICRAGDVTLKEGRPLIVVVRETPLHIGHLRQLLALAEIGAVILPPMPAFYTRPKTLDD
                     IVNHTVGRILDRLGVPHELTQAWTGTRTSRTAES"
     gene            complement(841..1335)
                     /locus_tag="AC16G0_00630"
     CDS             complement(841..1335)
                     /locus_tag="AC16G0_00630"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR5098154.1"
                     /translation="MTRSLGEALPDALLAMLDGRDLPGQMGKAILVATTDARGWPHPA
                     MLSYGEVVAMDRRRIRLAAYRESMTSGNLQRDGKLTLCLIDVGSVYYIKVQARTQQDP
                     MAGHAHLTRFEGVVEAVLEDRAREDLEPWAGVTSGITFRSGRPGVEVLRDWQAVLTGL
                     RSEG"
     gene            complement(1338..2117)
                     /gene="lgt"
                     /locus_tag="AC16G0_00635"
     CDS             complement(1338..2117)
                     /gene="lgt"
                     /locus_tag="AC16G0_00635"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR5098155.1"
                     /translation="MFASPGAIALQLGPLAIRWYGLLIATGVLLGSTLAHREAIRRGQ
                     NPDILLNGIVLTVLTALIGARLYYVLFNWDYYSAHPSKIVAVWEGGLAIHGGLIVGAV
                     TTALYCRHARLSLPMTTDILSPCVAIGQAIGRWGNFFNQEAFGTPTDLPWKLYIDPAH
                     RPPQFAGSDYFHPTFLYESVWNFLVFLLLWFGLRKRVESRPGALTLCYLGLYSIGRFF
                     VEGLRTDSLMLGSLRIAQVVSLILVVASGIGLLWMASKKKT"
     gene            complement(2387..2884)
                     /locus_tag="AC16G0_00640"
     CDS             complement(2387..2884)
                     /locus_tag="AC16G0_00640"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR5098156.1"
                     /translation="MPLILVLSLMLVLGTETSGFAQTLDIGQPAPELVLTDLAGNTHH
                     MSEYRGKGVLLNFWATWCVPCRTEMPSMERVYRALSEKGLVVLGVSLDAGPRGPVDDF
                     VKELALSFPILLDSPGKSTQTYRVFGLPTSFLIDRNGRLAGREVGARDWNGGEARKKL
                     EALLK"
     gene            complement(2976..3935)
                     /locus_tag="AC16G0_00645"
     CDS             complement(2976..3935)
                     /locus_tag="AC16G0_00645"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5098157.1"
                     /translation="MILITGATGFLGRHLVRRLVGAGETVRCLVRAESPGRAWLQAQA
                     VEVVPGHLLDAEAVAAACRGSRQIVHLAAPVHEGGDAVVERVNRQGAEILVDAARSAR
                     VERVLMVSPLGTASSAGLPFLRSRGAAEECLRESGLPFVILQSSLMFGRGDRLISGMI
                     RVLQRTGLLLVPGTGKTMLQPIWVGDMVSCLLRTLYDEGALDRTIPIGGPQHLTYEEV
                     ADQVAKMLNVPRMKIHLSRRAVAWIARLLEGVGQNPFLGYRHLELLEVGTITALDAVK
                     RSFGFQAMPLIEGVAYQLVPTTEHGAARPPSSVSRGSQAKDHR"
BASE COUNT          765 a         1361 c         1249 g          653 t
ORIGIN      
        1 cgtgaggatg atcaccaccg cacgcacttt ccggctgttt accatgcctg cctcctttcc
       61 ttagatcggg agaatagcgc atgacggcgg taagctcgac atgcgcagca agtgaggagc
      121 cattccggca tccgaacggc cgcccgcggg tgctggcgtt cttgccgcgg ggaccatcca
      181 ccgtcgaatt cgaagtggcg tggaagcgtg ctctgcgcct gggcacgctg gatgcggcag
      241 gcggaggagt ccataggtct catgactcgg ccgtgcgcga ggtccgcgtc ccggtccacg
      301 cctgggtcag ttcgtgcggg acccctagac ggtccaggat acggcccacg gtgtggttca
      361 cgatgtcgtc cagcgtcttg ggccgcgtgt agaatgcagg catggggggc aggatcacgg
      421 ctccaatctc cgccagggcc aggagctggc gcaggtgccc gatatggagc ggcgtctccc
      481 ggacgaccac gatgagggga cggccctcct tcaaggtgac gtctcccgcc cggcagatca
      541 aggtatccgc gtgacaattc gcgagcgccg agaccgtctt gacgctgcag ggagcgatca
      601 ccatcccggc cgtcctgaac gagccgctcg ccagggccgc cccgatgtcc ctgttgtcat
      661 agacgtgggt ggccagtcgc tccacgtctt tgatgctgta gtccgtctcc tccaggatgg
      721 tccgcttccc cgggccactg atgatcaggt gactctccag gtcgggaata tcccgcagca
      781 cctccaggag gcggatgcca tagatggatc cggtcgagcc agagatgccg acgaccagcc
      841 tcatccctca ctccgcagac cagtcagcac ggcctgccag tcccgcagga cctctacccc
      901 cgggcgcccg gagcgaaacg tgatcccgct ggtcacaccc gcccacggct ccaggtcttc
      961 cctcgcccga tcctcgagga ccgcctctac cacgccctcg aagcgggtga gatgggcgtg
     1021 ccccgccatg gggtcttgct gcgtcctggc ctgcaccttg atgtaataga cgctccccac
     1081 atcgatgagg cacaaggtta gcttcccgtc ccgctggagg ttccccgaag tcatactctc
     1141 ccggtaggcg gccaggcgga tgcggcgccg atccattgcc acaacctccc cgtacgacag
     1201 catggctgga tgcggccacc cgcgggcatc ggtcgtcgcc accagaatag ccttccccat
     1261 ctgcccaggc aggtcccgac catccagcat ggcgagcaag gcgtccggca gagcctcacc
     1321 cagcgaccgc gtcattgtca ggtcttcttc ttcgaggcca tccacagcag gccgatcccc
     1381 gaggccacga ccaggatcag gctcaccacc tgcgcgatcc ggaggcttcc gagcattaaa
     1441 ctgtccgtcc tgagaccctc cacgaagaag cgccctatcg agtacagacc cagatagcac
     1501 agggtcaggg ccccgggcct cgattctacc cgtttgcgca acccgaacca caggaggagg
     1561 aagaccagga agttccagac agactcatac aggaaagtcg ggtgaaagta atcggagccg
     1621 gcaaactggg gagggcgatg cgccggatcg atgtacagct tccaggggag atccgtcggg
     1681 gtcccgaagg cctcctgatt gaagaagttc ccccagcgtc ctatggcttg cccgatcgcc
     1741 acgcagggtg acaggatgtc cgtcgtcatc ggcagcgaga ggcgggcgtg ccggcagtag
     1801 agggcggtgg tgaccgctcc gacgatcaat cctccatgga tggcgaggcc gccctcccag
     1861 actgccacga tcttgctcgg atgggcgctg tagtagtccc agttgaagag gacatagtac
     1921 aggcgggccc cgatgagtgc ggtgagcacc gtgagcacga tgccattgag taggatatcc
     1981 ggattctggc ctcgccggat ggcctcccgg tgggccaagg tcgatcccaa gagaaccccg
     2041 gtcgcaatta acaggccgta ccaccggatt gccagcggcc cgagctgtaa tgcaatggct
     2101 cctggcgagg cgaacatggt tcctcccccg tgcctcaact tccaatcggg atgaacgccg
     2161 atcgcgtcga cgccgatgga ggctccctcg gtcagcgccg ggatgacgtc acgttacgcc
     2221 gttcacaata atcggggatt ccaggtcaag tcaagcggga agcgcaatcg aagctggcgg
     2281 ctcgtagccc atgcgagcgg ccagcctccc tccactggag gtgggtcgcc gcgccaagga
     2341 cgagcaggag tgcgagcagg atcatgggcg gaggaatgag ttgacgctac ttgaggagcg
     2401 cttccagttt cttgcgagcc tcaccgccgt tccagtcgcg agccccgact tcccgtccgg
     2461 cgagccggcc gttccgatcg atcaaaaagc tggtcggcag accgaaaacc cggtaggtct
     2521 gggtggactt tcccggggaa tccagcagga ttggaaaact gagggcaagt tctttcacga
     2581 agtcgtccac ggggccccgg ggaccggcat ccaggctaac ccccaggacg accagaccct
     2641 tctcgctgag ggcgcggtaa acgcgctcca tggagggcat ctccgtccgg cacggaacgc
     2701 accaggtggc ccagaagttg agcaggaccc ccttccctcg atactccgac atgtggtgtg
     2761 tgttgcccgc gagatcggtg aggactaact cgggcgccgg ctgaccgatg tccagggtct
     2821 gcgcaaaacc cgaagtttcg gtccccagca ccagcatcag gctgagcacc aggatgagcg
     2881 gcatgagtgc cctctacctc ccgtcggcga cacgacaaaa aaacccggcg gaccggacag
     2941 atccccgggc tcggacgatg cgaccacccc acgggctatc ggtgatcttt ggcctgcgac
     3001 ccccggctca cgctggaagg cggccgcgcc gccccgtgct ctgtcgtcgg gaccaactga
     3061 taggccacgc cttcaatgag gggcatcgcc tggaaaccaa agctacgctt gacggcgtcg
     3121 agggcggtga tcgtcccgac ttccaacaac tccaggtgcc ggtaccccag aaacgggttc
     3181 tgccccaccc cctccaggag tcgagctatc caggccacgg cccgacgcga gaggtggatc
     3241 ttcatacgtg ggacattcag catcttggca acctgatcgg cgacttcctc gtacgtcaga
     3301 tgctgtggcc cgccaatggg aatggtgcga tctagggcgc cctcgtcata gagggttcga
     3361 aggagacacg agaccatgtc cccgacccag atcggctgca gcatagtctt cccggtcccc
     3421 ggaaccagca agagaccggt gcgctggagc acccggatca tgccgctgat cagccggtcc
     3481 ccccgtccga acatcagaga ggattgcagg ataacgaaag gcaggccgct ctcccgcagg
     3541 cattcctcgg ccgcgccccg gctacggagg aacggaaggc ctgcggatga ggccgtcccc
     3601 aaggggctca ccatcaggac ccgctcgacc cgggcagacc gcgcggcatc caccagaatc
     3661 tcggccccct gacggttgac ccgctccacc acggcatccc cgccctcgtg aaccggtgcc
     3721 gcgaggtgaa cgatctggcg actcccgcgg cacgcagctg ccacggcctc ggcgtccagc
     3781 agatggcccg gcaccacctc gacggcctgg gcctgaagcc acgccctgcc gggagactct
     3841 gcccggacga gacatcgaac ggtctcgccg gcgccgacga ggcgccggac caggtgccgt
     3901 cccagaaatc cagtcgcgcc agtaatcagg atcatggctt ccgtatccgc tctccacccg
     3961 cgccggagac cgcgaatctg ctggagccgg cgatcgtgcc gccctctgtc tcacccggga
     4021 gcgctgcc
//
LOCUS       DBNGZC010000037         7258 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1763765, whole genome shotgun sequence.
ACCESSION   DBNGZC010000037 DBNGZC010000000
VERSION     DBNGZC010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7258)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7258)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7258
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1763765"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1781
                     /locus_tag="AC16G0_00650"
     CDS             147..1781
                     /locus_tag="AC16G0_00650"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5098158.1"
                     /translation="MSWSKSLEVRLLTAVCGVALLSIGIFAWLSIRYEQKAHIDLMMR
                     GASQFSDTVKRSIHYAMLQNRWVDAFQIMDTIGKQEGVTRVRVFNKEGLILFSTDRSE
                     VKSAVDKRAEACYGCHAAERPLERLNLPDRGRTFRDPEGNRQLGMITPLYNESGCSQS
                     CHIHPANQRVLGVLDITLSLAKADEEIASVTRRTAAFAAATILLLAAILAVVVRRGVV
                     RPVQELVEGTRLVAHGDLKHRLPVRTVDEVGVLAASFNRMTDALAKAQGELDALVQTL
                     EQRVEARTSELKETQAHLLQTERLASLGRLSASIAHEINNPLAGILTYAKLISRRLRA
                     GPPNTEETLTAVQQLGLVEREAQRCSSIVRNLRDFSRQRTPSFQAVDVGAVLAEALSL
                     LENRLAIQNVTVTREIEPLPPVQADFGQLRQACVNIIMNACEAMPEGGSLRVVAHEVL
                     SGSGGTAGSSPAGPCPRAPFIEVAVTDTGQGIPAEHLSKIFDPFFTTKEKGTGLGLSV
                     VYGIVEKHGGTIGVDSQVDHGTTVTIRLPVAAPSAA"
     gene            1788..2351
                     /locus_tag="AC16G0_00655"
     CDS             1788..2351
                     /locus_tag="AC16G0_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaemetzincin family Zn-dependent
                     metalloprotease"
                     /protein_id="HIR5098159.1"
                     /translation="MAGPGIILLQWMGEAPMDGGVLERIRGQVEAAFDIPVRLRTADD
                     PPGDSLDPRRRQHSSTKILRWILGSGLPAGAKVLGVTDVDLFIPVLTFVFGEAQLRGP
                     AAVVSVARLRRTYDDQPALEVTMEGRLLKECLHELGHTFGLAHCADGACVMSRSNTVL
                     DVDRKRAAFCRDCRLRLRTLTEEVEGA"
     gene            2348..3706
                     /locus_tag="AC16G0_00660"
     CDS             2348..3706
                     /locus_tag="AC16G0_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR5098160.1"
                     /translation="MKVKSCILVVDDEEIVRDSLASWLEEDGYQVDTAPDGQTAVAKL
                     AGQSYAILLVDLKMPGMDGLQVLAQARTLQPNAAVIIMTAYATVDTAVQAMKQGAYDY
                     LVKPFEPEELSLMVGKLTSQQSLRRENVLLRKALKRQFEFKDLVSKSPKMEAVFELAR
                     TAAKSNSTVLILGESGTGKELLARAIHAESPRHDGPFVGVSCAALTETLLESELFGHE
                     KGAFTGANSGARGKFEIAAGGTLFLDEIGDISAKLQMDLLRVLEAREFRRVGGSQVIK
                     TDVRIIAATNRDLKKLVDSGSFREDLYYRLKVIPVTLPPLRERKEDIPLLAEFLLSQF
                     RAEMRKPLEGVSSEALEVLMAYDWPGNVRELRNVLERGAVLAKGSILTPMELELTLPT
                     PVGGLAPTEPTDSLRDAERRHVAAVLKQNKWNISRSARTLGIDRVTLYNKIKRYQIRE
                     DE"
     gene            3977..4195
                     /locus_tag="AC16G0_00665"
     CDS             3977..4195
                     /locus_tag="AC16G0_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098161.1"
                     /translation="MRCPFFEEIVVAFCRAYPVKKMVPSDRIQANCVCTHEGFEDCAM
                     FREIMARMGPSAAAADRTEPERPGGPEA"
     gene            4242..4949
                     /locus_tag="AC16G0_00670"
     CDS             4242..4949
                     /locus_tag="AC16G0_00670"
                     /EC_number="1.4.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF013743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HIR5098162.1"
                     /translation="MVAILVVCTIVLFITADLVVQWLRRRRVSHAPSPKPQPTGPVDL
                     LLPSLQAERFALPGGLFFHRGHTWANVLFSGQVKVGVDDFFQKLIGRVDAITLPPMGV
                     EVKEGQPFAAVRQGGRTAMLTAPLDGVVCAVNSELAKMPGLLKRDPYTRGWLVAVRPT
                     DLAANLPRLTIGESAITWLKGELARLQEFLHITLTLDRDALVGATAADGGLMADGLLE
                     HLDEVTWKEFQSKFLGA"
     gene            5044..5373
                     /locus_tag="AC16G0_00675"
     CDS             5044..5373
                     /locus_tag="AC16G0_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c(7)-type cytochrome triheme domain-containing
                     protein"
                     /protein_id="HIR5098163.1"
                     /translation="MVWTVGVILALGGSAAAQQLRLPPDLVYAKAEGSPGKVVFSHQS
                     HVAYADKCTACHVKFFRILQPTRQVTHAAMEKGASCGTCHNGQMAFGPTDPAACIRCH
                     VGEGKPS"
     gene            5370..6116
                     /locus_tag="AC16G0_00680"
     CDS             5370..6116
                     /locus_tag="AC16G0_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5098164.1"
                     /translation="MKGAILFDSTKCIGCEACAAACKEKNQLPAAIDPKLTAYTWTVV
                     EQHNKAFVRRMCMHCESPTCASVCPVGAFSKRPDGPVVYDSSRCIGCRYCIMACPFDV
                     PKYQWDRAIPIVQKCDMCADRITAGQPTACASVCPTGATTFGDRDKLIAEARARVAAA
                     PQTYLPRIYGLEEVGGTSVLMLSSVPFTTLGFRPDLPLGPLPMLTWRVLSKIPDLAVV
                     GGAFLYGIWWITNRRTMVKEVMAREASKRG"
     gene            6144..>7258
                     /gene="nrfD"
                     /locus_tag="AC16G0_00685"
     CDS             6144..>7258
                     /gene="nrfD"
                     /locus_tag="AC16G0_00685"
                     /inference="COORDINATES: protein motif:HMM:NF015851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR5098165.1"
                     /translation="MKTDTGQMSAARWPEITFWRLVFLVVMATGTVAAYLRITRGLGA
                     STNLSDQFPWGLWIGFDMLCGVGLAAGGFAITASVYLFNLKRFHPIIRPTVLTTFLGY
                     LFAILGLLFDLGQPQRIWHAMIMWNPRSVMFEVAWCVMLYTTVLALEFSPVVFERLNL
                     EWPRRIIRAITVPLVILGVILSTLHQSSLGSLYLIVPEKLHALWYSPLLPVLFFTSAI
                     AAGLAMVIVESYLCQRAFNHHLEMTLLEPVARTMVVVLSVYGILRLQDLARRGVLASL
                     RNPGYEGTMFFLEIGLGVLLPILLLSIPRIRSTQPGMVTGAFLAVLGFVMHRLNVSVT
                     GMERASRVSYFPSWMEIAVSLALVAAGFALFGLAVRYL"
BASE COUNT         1286 a         2242 c         2378 g         1352 t
ORIGIN      
        1 cactgttgaa tttttcggca ctcggcatgc actctaccaa agtcaatggc aatggcgatg
       61 gttttgtggg gcggactgtc ccaggttcgc tttggactgg gacagctacg ggttcgtcaa
      121 cagtataaac ccatatagaa ctggatatgt cctggtcgaa aagtctggaa gtcagactct
      181 tgacggcggt gtgtggcgtt gccctgctgt ctatcggtat cttcgcgtgg ctcagcattc
      241 gctatgaaca gaaagcgcac atcgatctga tgatgcgggg agcgagccag ttcagcgata
      301 ccgtcaagcg cagcatccac tacgcgatgc tgcagaatcg ctgggtggat gcctttcaga
      361 tcatggacac catcgggaag caggaagggg tcacccgggt tcgagtgttc aacaaggagg
      421 gcttaatcct gttctccacg gatcgctcgg aggtgaagag cgcggtggac aaacgggccg
      481 aggcctgtta tggctgccat gccgccgagc ggccactcga gcgcctgaat cttcccgacc
      541 gcggacgcac gttccgggat ccggaaggga atcgtcaact cgggatgatc acccccctgt
      601 ataacgagtc gggctgttcg cagagctgcc acattcatcc ggcgaaccag cgggtcctgg
      661 gtgtcctgga catcaccctt tccctggcca aggcggacga ggagatcgcc agcgtcacgc
      721 gtcgcacggc cgccttcgcc gccgccacca tacttcttct ggccgccatc ctggcggtgg
      781 tggtgcggag gggggtcgtg cggccggtcc aggaactggt ggagggcacc cgcctggtcg
      841 cgcatgggga cctgaagcac cggctccccg tgcggaccgt ggacgaggtc ggcgtgctgg
      901 cagcctcctt caaccgcatg accgatgccc tggcgaaagc ccagggagag ttggacgccc
      961 tggtgcagac gctggagcag cgggtggagg ctcgcaccag cgagctgaaa gagacgcagg
     1021 cccacctgct tcagacggag aggcttgcct ccctcggcag gctgtccgcc tctatcgccc
     1081 acgagatcaa caacccgctt gccgggatcc tcacgtatgc caaactcatc agccggaggc
     1141 tccgggcggg cccgccgaac accgaggaga cgctcaccgc cgttcagcag ctcggcctgg
     1201 tggagcggga agcgcagcgc tgttccagca tcgtccggaa tctccgggat ttctcccgac
     1261 agcggacgcc gtcgttccag gccgtggacg tgggggcggt gctggccgag gccctctcgc
     1321 tactggaaaa tcggttggcc atccagaacg tgacggtgac gcgagagatc gagccgctgc
     1381 ctccggtgca ggccgatttc ggccagctcc ggcaggcgtg cgtgaacatc atcatgaacg
     1441 cctgtgaggc catgccggag gggggatctc tgcgggtggt cgcgcacgag gtcttgtccg
     1501 gaagcggagg gacggcaggc tcctctccgg cgggaccctg tccacgagcc ccgtttatcg
     1561 aggttgccgt caccgacacc gggcagggca taccggctga gcatctctcc aagatttttg
     1621 atcccttctt cacgacgaag gagaaaggga ccggcctcgg gctctcggtg gtgtatggga
     1681 tcgtggagaa gcacggcggc acgattgggg tggacagcca ggttgaccac ggcaccacgg
     1741 tgaccatccg tctgccggtg gcggccccca gcgcggcgtg aggcaccatg gccgggccgg
     1801 gcatcatcct gctccaatgg atgggtgaag cgccgatgga tggcggggtg ctggagcgaa
     1861 ttcgcgggca ggtcgaggcc gccttcgaca tccccgtccg tctccgtacg gccgacgacc
     1921 cgccaggaga cagcctcgat ccccgccgcc ggcagcattc ctcgaccaag attctccgct
     1981 ggattctggg ctccgggctg ccggcggggg ccaaggtcct gggggtcacg gatgtggatc
     2041 tcttcatccc tgtcctgacc ttcgtcttcg gagaagctca actgcgtggc ccggcggcgg
     2101 tggtgagtgt cgcccgcttg cgccggacct acgatgatca accggcgctc gaggtgacga
     2161 tggagggccg tctcctgaaa gagtgcctgc acgaactggg gcacacgttc ggcctcgccc
     2221 actgtgcgga cggggcatgc gtgatgtccc gctcgaacac ggtgttggat gtggaccgca
     2281 agcgcgccgc cttttgccgt gactgccgcc tgcgcctgcg caccctgacc gaagaagtgg
     2341 agggagcatg aaggtcaaga gctgcatcct ggtggtggac gatgaggaga tcgtccggga
     2401 ctcgctggcc agctggctgg aggaggacgg ctaccaagtc gacaccgcgc cagacgggca
     2461 gaccgccgtg gccaagcttg cggggcagtc ctacgccatc ctgctggtgg atctgaagat
     2521 gccggggatg gatggcctcc aggtcctggc gcaggcccgc accctccagc cgaacgcggc
     2581 cgtgatcatc atgaccgcct atgcgacggt ggacaccgcc gtccaggcca tgaagcaagg
     2641 ggcgtacgat tacctggtga agcccttcga gccggaagag ctgtccctga tggtggggaa
     2701 gctgacaagc cagcagagcc tccggcggga gaacgtcctg ctccgcaagg ccttgaagcg
     2761 tcagtttgag ttcaaagacc tggtcagcaa gagtcccaag atggaggcgg tcttcgagct
     2821 ggcccggacg gcggccaaga gcaattcgac cgtcctgatc cttggcgaga gcggaaccgg
     2881 gaaggaactc ctggcccggg ccatccacgc ggagagcccg cgacacgacg gccccttcgt
     2941 gggggtctcc tgcgcggcgt tgaccgagac cctgctggag agcgaactct tcggccacga
     3001 gaaaggggcc ttcaccgggg ccaacagcgg cgcccgaggg aaatttgaaa tcgcggccgg
     3061 cgggaccctg ttcctggacg agattggcga catcagcgcc aagctgcaga tggatctcct
     3121 gcgggtcctg gaagcgcgcg agttccgtcg agtgggcggc tcgcaggtca tcaagaccga
     3181 tgtgcgcatc atcgcggcaa cgaaccggga tctcaagaag ctggttgact cgggaagctt
     3241 ccgggaggat ctctattacc gtctcaaggt catcccggtg acgcttccgc ccctgcgcga
     3301 gcggaaggaa gatatcccgc tcttggccga gttcctcttg agccagttcc gcgccgagat
     3361 gcgaaaaccg ctggaggggg tttccagcga ggcgctggag gtgctcatgg cctatgattg
     3421 gcctggtaac gttcgggagc tcaggaatgt tctggagcga ggagccgtcc tggccaaggg
     3481 ctcgattctc acccccatgg aattggagct cacgctgccg acacctgtcg ggggactggc
     3541 gccgaccgag ccgaccgact ccctccggga tgcggaacgc cggcatgtgg cggcggtact
     3601 caaacaaaac aagtggaaca tcagccggtc ggccaggacc ctcggcatcg accgggtaac
     3661 tttgtacaat aaaatcaagc gctatcaaat acgcgaagac gaatgaggag aggggttgct
     3721 gagaaactcc acagtaccat tgtcgttctc aacacttgca acaatccccc acaaaccgtg
     3781 gagttaggca attatcgaga ataggacgta aaggatttca acacccacct gtccatttga
     3841 tttacatcac tcattccagc ctttcccatc aaacctttag aacacagaga ctttcaggcg
     3901 ccatgacggg tgcgcccgtg atggcatccc aattgtaatg gcagcaagta gaaccgactt
     3961 actggaggca attcccatgc gctgtccgtt cttcgaagaa attgtggtgg ccttctgccg
     4021 ggcataccca gtgaagaaga tggtcccgag cgatcgaatc caggccaact gcgtgtgcac
     4081 ccacgagggc ttcgaggatt gcgcgatgtt tcgcgagatc atggcgagga tggggccctc
     4141 cgcggccgcg gcggaccgga ccgagcccga gcgtccggga ggccctgaag catgaggctg
     4201 ctgccggaat gaccggcacg agtgccgaaa gggggtggat catggttgcc atcttggttg
     4261 tgtgcacgat tgtcctgttc atcaccgcgg atctggtggt gcagtggctg cggcggcggc
     4321 gcgtgtcgca cgcgccgagt cctaagcccc agccgaccgg gccggtggac ctgctcttgc
     4381 ccagcctcca ggcagagcgc ttcgcgcttc cgggcgggct cttctttcac cgagggcaca
     4441 cctgggcgaa cgtcctcttc tccggccagg tgaaggtggg tgtggacgac ttcttccaga
     4501 agctcatcgg gcgggtggac gccatcacgc tgccgcccat gggtgtcgaa gtgaaggagg
     4561 ggcagccgtt tgcggccgtc cgtcagggtg ggagaaccgc catgctgacg gccccgctgg
     4621 acggggtggt ttgcgccgtc aactcagagc tggccaagat gcccgggctc ctcaagcggg
     4681 atccctacac ccgcggatgg ctcgtggccg tacggccgac cgacctggcc gccaatctcc
     4741 cgcggctcac gatcggggag agcgcgataa cctggctcaa gggagagctg gcccgcctgc
     4801 aggagttcct gcacatcacc ttgacgcttg accgggatgc cctggtcggg gccacggctg
     4861 cagacggcgg tttgatggcc gacggcttgt tggagcatct ggacgaggtc acctggaagg
     4921 aatttcagtc caagttcctg ggtgcctgag cgctaccgct gctctgggtc gacgcggcac
     4981 gtcccccttc tcgggatcag gccgccggag ggtgaaacgg tgagatgcag atggagtgca
     5041 agtgtggtct ggacggtggg tgtcatcctt gccctggggg gctctgcagc cgcccagcag
     5101 ctccgcctgc cgccggacct ggtgtacgcc aaggccgagg gcagtcccgg aaaggtcgtc
     5161 ttcagccacc agtctcacgt ggcctacgcg gacaagtgca cggcctgtca tgtgaaattc
     5221 ttccgcattt tgcagcctac ccggcaggtg acgcatgccg ccatggagaa gggcgcgtcg
     5281 tgtggcacct gccataacgg ccagatggcc ttcggcccca cggatccggc cgcctgcatc
     5341 cggtgccacg tgggtgaggg gaaaccgtca tgaagggcgc catcctcttc gacagcacca
     5401 agtgcatcgg atgcgaggcc tgcgctgctg cgtgcaaaga aaagaaccag ttgcccgcag
     5461 ccatcgatcc gaagttgacg gcctatacct ggacggtcgt cgagcaacac aacaaggcct
     5521 tcgtccggcg gatgtgcatg cactgcgagt cgcctacctg cgcctcggtt tgtccagtgg
     5581 gggccttcag taagcggccc gacggcccag tggtctacga ttccagccgg tgcatcggct
     5641 gccgctactg catcatggcg tgccccttcg acgtcccgaa gtaccagtgg gaccgggcga
     5701 tccccatcgt ccagaaatgc gacatgtgcg ccgatcgaat cacggccgga cagccgacgg
     5761 cctgcgcatc cgtctgcccc accggggcga cgacctttgg cgatcgagac aagctcatcg
     5821 cggaggcgcg ggcgcgggtt gcggccgccc ctcagacgta cctgccgcga atctacggcc
     5881 tggaagaagt cggcggcacc tcggtcttga tgctctccag tgtgccattc accacgctgg
     5941 gcttccggcc agacttgcca ctcgggccgc tgcccatgct cacctggaga gtcctttcga
     6001 agatcccgga cttggccgtg gtggggggcg cgttcctgta cggtatctgg tggatcacca
     6061 accgccggac catggtcaag gaagtcatgg cgcgggaagc gtcgaaacgg ggctgatccg
     6121 tcgaggcaaa tgggggcagg catatgaaaa cagatacggg gcagatgtcc gcggcgcggt
     6181 ggcccgagat caccttctgg aggctcgtgt ttctggtcgt gatggccacc gggaccgtgg
     6241 cggcgtacct acggatcacg cggggcctgg gggccagcac caacctcagc gaccaatttc
     6301 cctggggcct ctggatcggc ttcgacatgc tctgcggggt ggggttggcg gcgggcgggt
     6361 ttgctatcac cgccagcgtc tacctcttca acctgaagcg gtttcatccg atcatccgac
     6421 ccacggtgct cacgacattt ttggggtacc tcttcgccat tctgggtctc ctcttcgatt
     6481 tgggccagcc acagcggatc tggcatgcca tgatcatgtg gaatccccgc tcggtcatgt
     6541 tcgaggtggc ctggtgtgtg atgctgtaca ccacggtgct tgccctggaa ttctctcccg
     6601 tcgtctttga gcgcctcaac ctcgagtggc cccggcgaat catccgagcc atcaccgtgc
     6661 ccctggtgat cctgggcgtg atcctctcca cgcttcacca atcctcgctc ggctcgctct
     6721 acttgatcgt gccggagaag ctgcacgcgc tctggtattc gccgctcctg cccgttttgt
     6781 tcttcacgtc ggccattgcc gcaggcctgg cgatggtaat cgtggagtcc tacctctgcc
     6841 agcgcgcctt caatcaccat ctggagatga ccctgctgga accagtggca cggaccatgg
     6901 tggttgtgct cagcgtctac ggcatcctga ggcttcagga tctggcccgc aggggggtcc
     6961 tggcgagcct ccgcaacccc gggtacgagg gcacgatgtt cttcctggag atcgggctcg
     7021 gcgtcctcct cccgatcctc ttgctctcaa tcccccggat ccgatcgacc cagccgggga
     7081 tggtgacggg cgccttcctg gccgtcctgg gcttcgtcat gcaccgactg aacgtcagcg
     7141 tcacgggcat ggagcgggcg tcacgggtca gttacttccc gtcctggatg gagatcgccg
     7201 tttccctcgc cctggtggcg gcgggtttcg cgctctttgg cttggccgtc cggtatct
//
LOCUS       DBNGZC010000038         1076 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1763854, whole genome shotgun sequence.
ACCESSION   DBNGZC010000038 DBNGZC010000000
VERSION     DBNGZC010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1763854"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="AC16G0_00690"
     CDS             complement(<1..418)
                     /locus_tag="AC16G0_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR5098166.1"
                     /translation="MSFEELGLHADLVKVTQRLGFEHPTPIQRLAIPPLMQGRDVLAT
                     AVTGSGKTAAFLLPILHDLMGKRRGTTRALVVAPTRELAAQIADHVRDLAGHTGLTGA
                     AVYGGVAMGPQEHAFRKGVDVLIATPGRLLDHFQYPY"
     gene            783..>1076
                     /locus_tag="AC16G0_00695"
     CDS             783..>1076
                     /locus_tag="AC16G0_00695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5098167.1"
                     /translation="MAADRDAALTVINTAARWYREFLPPEELHDPEMTPAQWEAEAQR
                     MTWYGAFVEGVLVGVMGLEYVRDAALLRHAYVLAGHQRGGIGTVLREHLEAQVR"
BASE COUNT          183 a          337 c          361 g          195 t
ORIGIN      
        1 catagggata ctggaaatgg tcaaggagcc gtccgggggt cgctatcagc acgtccaccc
       61 ctttccggaa ggcatgctcc tgcggcccca tggcaacgcc tccatacacc gccgccccgg
      121 tgagaccggt gtgacccgcg aggtcgcgga cgtgatcggc tatctgtgcg gccagctcgc
      181 gtgtcggggc cacgaccagc gccctggtgg ttccccggcg cttgcccatc aggtcgtgca
      241 ggatggggag gaggaatgcg gccgttttgc cgctgcccgt cacggcggtg gccagcacgt
      301 cccggccctg catgaggggt gggatggcca gccgctggat gggcgtgggg tgctcgaacc
      361 ccaaccgctg ggtgaccttg acgagatctg cgtggagtcc gagctcttcg aacgacatac
      421 ctgtgcctgt gctttccgcg aacacaccaa acccgggtat gggttctgcg tgctcgctgc
      481 tatgtccggc tgagtggatt tgaaagggat aacggtggga tcgccggtga agccgccgtt
      541 caggctctga gacctgccct acgctgtggg tcccgctcca gtgcctgaaa cattggcttc
      601 cgtcgcggtg agccggggga caataccaca ccaggcgaga ttcaccaagg gttattccgg
      661 caacggcgga aaaagtcccc ggactgccag ggggagagca tccttcgtgt gaaatcgcct
      721 tgctcgatga gcgcagaggg tgcgatactt ccgctatggt cgaaccgaag atccgctccc
      781 tcatggccgc cgaccgagac gccgcgctta ccgtgatcaa tacggctgcg cgctggtacc
      841 gggaattcct gccccctgag gagctccacg atcccgagat gactcccgcc cagtgggagg
      901 ctgaagcgca gcgcatgacc tggtacggcg ccttcgtcga gggggtgctg gtcggcgtca
      961 tgggactgga gtatgtaagg gacgccgccc tcctccgcca cgcctacgtc ctggctggac
     1021 accagcgcgg cggtatcggc acggtgctgc gcgaacacct tgaagctcag gttcgg
//
LOCUS       DBNGZC010000039         2457 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1763863, whole genome shotgun sequence.
ACCESSION   DBNGZC010000039 DBNGZC010000000
VERSION     DBNGZC010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1763863"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1473)
                     /locus_tag="AC16G0_00700"
     CDS             complement(<1..1473)
                     /locus_tag="AC16G0_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098168.1"
                     /translation="MESLFSALWYRVADLKPRIRSHANIHRHRYRGETWYVLHDLASG
                     RAHRFSPASYLVIGLMDGRQTIQEIWDTAMGRLGDDAPTQDEMIQLLSQLHSADVLQC
                     DVSPDAAELFQRAEKQQRKKMFGQLMSPLWWRVPLVDPERFLTRLLPWARPFIGPAGA
                     CVWAIVVGVALVLTGVHWKDLTSDLLDRMLMPSNLLILWCLFPFLKAFHEIGHGLAIK
                     AFGGEVHDMGVMFLVFSPLPYVDASTASAFSSKWQRVVVGAGGMMAELFLAALALFVW
                     LAAEPGLVRSLAYNAVLIAGLSTVLFNGNPLLRYDGYYILADLLEIPNLYQRSRTYLG
                     YLCERYLFGHREAERPPDSPSERVWFVIYAVSSFIYRIFVIIAISLFLFYKAFYLGMV
                     AVAARVGAWLVVPAWKIVHFLLASPRIRRVRVRAIAISTVLVALVVVGITLVPIPSRT
                     GAEGIVWVPEDALVRAAADGFVERLVARPGSHVRPGDLRLV"
     gene            complement(1473..>2457)
                     /locus_tag="AC16G0_00705"
     CDS             complement(1473..>2457)
                     /locus_tag="AC16G0_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5098169.1"
                     /translation="TLERPANQPFEPATVEVCEATAALAGPVLEIQRREDRWLLTKAG
                     DSTRLLLGKVIGPGHLILKLSLLAAAGLGLFLAFATGEYRVSATATLEPVVKRAVAAP
                     FAGYLAEAPIRAGDVVRKDQVLARLDDRELKLQRLKWGSQYEQLAKQHQQALAQRNAA
                     QVAVLAAQMDQARAEVALLEDQLARIQMRSSIEGLVVTGDLSQSLRAPVERGQVLFEV
                     APLDAFRVILQVDEHSITDVRLGQTGSLVLSGMPHEAFPFRVAKITPVSTAREGRNYF
                     RVEATPEGIPEHLRPGMEGVGKVSVDNRLLVWIWTRDVINWVRLKIWTWLP"
BASE COUNT          481 a          838 c          777 g          361 t
ORIGIN      
        1 caccagccgg aggtcgcccg gacgcacgtg cgatccgggt cgcgccacca gccgctcgac
       61 aaagccgtcc gcggcggccc gcaccagcgc gtcctcaggc acccacacga tcccctccgc
      121 gcccgtccgg gaggggattg gcacgagggt aatccccacg accaccagag ccaccaacac
      181 cgtgctgatc gcgatggccc ggacgcgaac gcgccggatg cgcgggctgg ccaggagaaa
      241 gtggacgatc ttccaggcag gcacgacaag ccaggcaccc acgcgcgccg caaccgcgac
      301 catccccagg tagaaggcct tgtaaaacag aaagagcgaa atcgcgatga tgacgaagat
      361 ccgatagatg aaggagctca ccgcatagat gacgaaccag actcgctccg acggagagtc
      421 cggaggccgc tccgcctccc gatgtccgaa gaggtatcgc tcgcacaggt acccgagata
      481 ggtgcgagag cgctggtaca ggttggggat ctccaggaga tccgccagga tatagtaccc
      541 gtcataccga agcagcgggt tcccattgaa caggaccgtg gagagaccgg cgatgagcac
      601 ggcgttgtag gcgagacttc tgacgagccc gggctcggct gccagccaca cgaaaagggc
      661 cagggccgcc aggaacagct ccgccatcat gcccccagcg cccaccacaa cccgctgcca
      721 cttgctggaa aaggccgagg ctgtggaggc gtcgacgtac ggcaagggcg agaacaccag
      781 gaacatcacc cccatgtcgt ggacctcgcc gccgaaggcc ttgatggcca gcccgtggcc
      841 gatctcgtgg aacgccttga ggaagggaaa caggcaccag aggatcagca gattactcgg
      901 cattaacatt cggtcgagga ggtcggacgt caaatccttc cagtggactc ccgtcaggac
      961 gagggcgacc ccgaccacga tcgcccagac gcacgcgccg gccggtccga tgaacggccg
     1021 cgcccagggc agcagacgcg tcaagaatcg ctcggggtcc accagtggaa cccgccacca
     1081 gagcgggctc atcaactgcc cgaacatctt tttccgctgc tgcttctcgg cgcgctggaa
     1141 gagttcggcg gcgtcggggg agacatcgca ttgcaggaca tcggcgctgt ggagctgact
     1201 caaaagctgg atcatctcgt cctgggtcgg cgcgtcgtcc cccagtcgtc ccatggccgt
     1261 gtcccagatt tcctggatgg tttgccggcc gtccatgagg ccgatgacga ggtagctggc
     1321 cggcgaaaac cgatgggccc ggcccgaggc cagatcatgc aggacatacc acgtctcgcc
     1381 gcggtagcgg tggcgatgga tgttggcatg gctgcggatc ctcggcttga gatccgccac
     1441 ccggtaccac aaggcgctga aaagcgattc catcagggca gccaggtcca aatcttcagg
     1501 cgcacccagt tgatgacatc gcgcgtccag atccagacca gcaggcgatt atccaccgaa
     1561 actttcccga ccccctccat gccaggccgc aggtgctcgg gtatgccttc cggcgttgcc
     1621 tccacccgga agtaattccg cccctcccgt gcggtcgaga ccggcgtgat ctttgccacc
     1681 cggaacggga atgcctcgtg gggcatgccg gagaggacca gcgatccggt ctgccccagc
     1741 cggacatcgg tgatcgagtg ctcatccacc tgcaggatca cccgaaacgc atccagggga
     1801 gccacctcga agagcacctg gccgcgctcc accggcgccc gaagcgactg gctgaggtcg
     1861 ccggtcacca ccagaccctc gatggaggag cgcatctgga tccgcgccaa ctgatcctcc
     1921 agcagggcca cctcggctcg cgcctggtcc atctgagcgg ccagcacggc cacttgcgcc
     1981 gcgttccgct gggccagggc ttgctggtgc tgcttggcca gttgctcgta ctggctcccc
     2041 cattttagtc gctggagctt gagctcccga tcgtccagtc tggccagcac ctggtccttc
     2101 cgcaccacat ccccggcgcg gatcggcgcc tcggcgagat aaccggcgaa gggggccgcc
     2161 actgcccgct tgacaaccgg ctccagcgtc gcggtcgcgg atacccggta ctctcccgtg
     2221 gcgaacgcga ggaagagtcc gaggcccgcg gcggccagaa gcgagagctt caggatcaga
     2281 tggcccggcc cgatgacctt gcccaagagc aagcgggtcg agtctccggc cttggtgagc
     2341 agccaacgat cctcacgacg ctggatctcg agcacggggc cggccagtgc cgcggtggcc
     2401 tcgcacactt cgacggtcgc cggctcgaac ggctgattcg cggggcgctc cagcgtg
//
LOCUS       DBNGZC010000040         2176 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_198549, whole genome shotgun sequence.
ACCESSION   DBNGZC010000040 DBNGZC010000000
VERSION     DBNGZC010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_198549"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="AC16G0_00710"
     CDS             <1..439
                     /locus_tag="AC16G0_00710"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR5098170.1"
                     /translation="DPDDIDQMTAVLEEQARQIADGGTGMEADSAFHLLLAQAAKNKV
                     ILKLNESIVDSLRETRERSLQTHGRPARSLAGHRKILEAIRTKNPAKARRAMLAHLRA
                     IERNVLKPCALKEKGGEGGGVKGEDAVGIMNPDGKLGSLPQEG"
     gene            442..1608
                     /locus_tag="AC16G0_00715"
     CDS             442..1608
                     /locus_tag="AC16G0_00715"
                     /inference="COORDINATES: protein motif:HMM:NF026048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster-binding protein"
                     /protein_id="HIR5098171.1"
                     /translation="MTGSVEVVYRGIFQKTLGQRITRAIVLAAWKEGKVGMSFGRYGD
                     SPERNGIPAKSFAVVADTEEELQAFLARYEPTNNDVTVAVDDTLCKGVESWAWYGLQP
                     INKLTRPGGTVLVTSMQTPEELLKDCHRKGAEWTLGIVKGTASVSGLWVYKDDHTDVR
                     VLGAIAKVAPQVVKLASIEAIIRDEWKDELKVASARKAYERVSVRKVGPAEGNPDKPY
                     EFQLLKWWEMKEGLVIPGIPVKQEVPGWTGGYRPGRNPTFKKYATRTMRPVVNFDTCT
                     KCTLCWLQCPDSCFDITPTGHYDANMEACCGCGVCEAVCPVEDCITMVNEQAFEDNAS
                     QFEAYERDKETYLTSWLQEKIKDKVITHRSHGFRYRGQYEEELAEAAADGGGDD"
     gene            1708..>2176
                     /locus_tag="AC16G0_00720"
     CDS             1708..>2176
                     /locus_tag="AC16G0_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HIR5098172.1"
                     /translation="MSVATVAAPAVQTGAATEKEMLISGCAAIAQALRLASIDVVTAY
                     PIRPYDTVMQAVAKLIANGELDAEYIVAASEHDQFEIVKHASAVGGRVFCGSSGVGWM
                     YAMEALAVTPALRLPLVALVGNRALDDPGAFGCEHNDALCVRDLGWMLTWVDTA"
BASE COUNT          423 a          650 c          757 g          346 t
ORIGIN      
        1 cgatccggat gatatcgatc aaatgacggc ggtcctggaa gagcaagcgc ggcagatcgc
       61 cgacggcggg accggaatgg aggcggactc cgccttccat ttgctgttgg ctcaagcggc
      121 gaagaacaag gtcatcctga agctgaacga gtcgatcgtg gacagcctgc gtgagactcg
      181 ggagcgttcg ctccagacgc acggccgccc cgcacgctct ctggccggcc accgcaagat
      241 cctggaggca attcgcacta agaaccccgc caaggccagg cgggccatgc tggcgcatct
      301 tagagccatc gaaaggaatg tgttgaagcc gtgtgctctc aaagagaaag gaggagaggg
      361 gggcggggtg aagggtgaag atgccgtggg catcatgaat cctgatggga agctcggttc
      421 cttaccacag gagggttgag catgaccggt tcggttgaag ttgtgtatcg cggcatattc
      481 cagaaaactc tgggccagcg gatcacccgt gccatcgtcc tggcggcctg gaaggaaggg
      541 aaggtcggga tgtccttcgg gcggtacggg gattcgcctg agcgcaacgg tatccccgcc
      601 aagagcttcg cggtggtggc ggataccgag gaggagttgc aggccttcct cgcccgctac
      661 gagcccacta acaacgacgt cacggtggca gtggatgaca ccctgtgcaa gggggtggag
      721 tcctgggcct ggtacgggct gcagccgatc aacaaactga cccggcccgg cgggacggtc
      781 ctcgtgacct ccatgcagac accggaggag ttgctgaagg actgccaccg aaaaggcgcg
      841 gagtggactc tcgggatcgt gaagggcacg gccagcgtct ccggcttgtg ggtctacaag
      901 gacgaccaca ccgacgtgcg ggtcctgggg gccatcgcca aggtcgcccc gcaggtggtc
      961 aagcttgcgt ctatcgaggc gatcatccgc gatgagtgga aggacgagct gaaggttgcc
     1021 tccgcccgga aggcgtacga gcgggtgagc gtccgcaagg tgggaccggc cgaagggaat
     1081 ccggacaagc cctacgagtt ccagctcctg aagtggtggg agatgaagga gggcttggtc
     1141 atcccgggca tcccggtcaa gcaggaggtc ccggggtgga ccggcgggta ccgcccgggc
     1201 cgcaacccca ccttcaagaa gtacgcgacc cgcaccatgc gcccggtggt gaacttcgac
     1261 acctgcacca agtgcaccct gtgctggctc cagtgccccg actcctgctt cgacatcacc
     1321 cccaccggtc actacgacgc caacatggag gcctgctgcg gctgcggggt ctgtgaggcg
     1381 gtctgcccgg tggaggactg catcaccatg gtgaacgagc aggccttcga ggacaacgcc
     1441 agccagttcg aggcctacga gagggacaag gaaacctacc tcacctcctg gctgcaggaa
     1501 aagatcaaag acaaggtgat cacccatcgc tcacatgggt tccggtaccg cgggcagtac
     1561 gaggaggagc tggccgaagc ggcggcggac ggcgggggcg acgattgagc ttcggatgcg
     1621 cgcccggctg gtcccgggcc gccggccgcc cgcttttcgg cctgcggcca ggaatgtctc
     1681 caaccgatga cgactggagg tatcaacatg agcgtagcga ctgtagcggc acccgcggtc
     1741 cagacgggag ccgcgacgga gaaggagatg ctgatcagcg ggtgtgcggc catcgcccag
     1801 gccctgcgcc tggccagcat cgacgtggtg acagcctatc ccattcgccc ctatgacacc
     1861 gtgatgcaag cggtggccaa gctcatcgcc aacggcgagt tggatgccga gtacatcgtg
     1921 gctgccagcg agcacgacca gttcgagatc gtgaagcacg cctccgcggt gggaggacgc
     1981 gtcttctgcg ggtcgagcgg cgtcggctgg atgtatgcca tggaggcctt ggccgtgacg
     2041 ccggccctgc gtctgcccct cgtggcgttg gtcggcaacc gggccctgga cgatccggga
     2101 gccttcggct gcgagcacaa cgacgccctc tgcgtgcggg atctcgggtg gatgctcacc
     2161 tgggttgaca ccgcgc
//
LOCUS       DBNGZC010000041         6748 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1763886, whole genome shotgun sequence.
ACCESSION   DBNGZC010000041 DBNGZC010000000
VERSION     DBNGZC010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6748)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6748)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6748
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1763886"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="AC16G0_00725"
     CDS             complement(<1..318)
                     /locus_tag="AC16G0_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098173.1"
                     /translation="MAPRVLILDGFWNKTLAAVRSLGRRGFFAAVGETTRFAPAAFSR
                     YCRRKLVYPSVARQPKDFLAALERELATGQYDVLFPMELSTQLLVTRNRERLEKLVRI
                     PFAD"
     gene            complement(336..1691)
                     /locus_tag="AC16G0_00730"
     CDS             complement(336..1691)
                     /locus_tag="AC16G0_00730"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR5098174.1"
                     /translation="MNLLLVSPLTSKSLLGADFYFRMPTLGLLRVAGATPPDWTVTIL
                     DEKVEPIDFSRSVDLVGITGMTPAINRAYEIADTFRARGVPVVMGGIHVSMLPEEALA
                     HSDAVVVGEAEDLWPRLLSDFRQGALQRCYRHQDFPPLSQLGSPRWSLYDGKRYLPFH
                     CVETSRGCPHGCDFCSVTNYFGGCYRTRPPEEVEQEIRGLRPFEGRFTLKNVVFFVDD
                     NIVGKRTHARDLLTRIIPYQLKWVGQASTTIARDDELLELCRKSGCMGLLIGFETLSK
                     ERLKAVRKGFNSPEGYMDAIHKIHDFGIGVSGAFVFGLDGDDPGVIDRTYEFVQKAKL
                     ESPYFSILTPYPGTRLHQKLYGEGRIFDLDWSNYNTNNVVYRPMGMTARELFDGYYRL
                     QMAVHTVPAILQRFWGTTSKANFWLPMNYGFRRSIKKLAARAGEFRPVESVVGDTLQA
                     S"
     gene            complement(1688..2779)
                     /locus_tag="AC16G0_00735"
     CDS             complement(1688..2779)
                     /locus_tag="AC16G0_00735"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HIR5098175.1"
                     /translation="MFSRIGAIQLPVPRLHLELTNRCNFSCEFCPDRSMRRPRGTMPL
                     SMVERLLAQAGQEGLARQVHFHVMGEPLCYPALPEVVRLARRHGMQAWVTTNGSLLTP
                     ELLSELQAAGLFHLSISLQTPDAPTFGRRGSGLLSFEQYRERLISTTHAFLGQPSGMH
                     LSIGFLSNPLRRFHAPGASGIRAAESGRELRAHMARWVEWIFSGTALDRQVPALLARL
                     KGAGILKENRVPLTEDLDFRVRALGNWAGHFEGRNVPARFGYCPGLSENFGILWNGDY
                     VICCADYDGKTVLANCSQTPLRDYLSLPAVQEIAEGFRRYRVIHPYCRQCLGDGHPVS
                     AILRQAGSILYFKVYRKLMHGDTVGREAA"
     gene            complement(2781..3485)
                     /locus_tag="AC16G0_00740"
     CDS             complement(2781..3485)
                     /locus_tag="AC16G0_00740"
                     /inference="COORDINATES: protein motif:HMM:NF015510.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA family protein"
                     /protein_id="HIR5098176.1"
                     /translation="MLAAFLFSWGPLGPGDVAPAEIPILGKERLEVIEKLQERQRAVT
                     SVRATVIQKKRHPLLAAEVVSEGTLLFKRPNLVRWEVNKPDRTIILIDDRWLVTYHPD
                     RREAERRDLRDDFGARAAVDFFTSGMSLAVVDLEKRFQVDLYRDDELLVIRLTPRSRF
                     VAQAVASVTISQRATDAIPRQIVVLGQKGDRTELTLSSVSINPHVPEDAFTLHLGPEV
                     RVTDAGKLASEGGSDR"
     gene            complement(3529..5049)
                     /gene="tes"
                     /locus_tag="AC16G0_00745"
     CDS             complement(3529..5049)
                     /gene="tes"
                     /locus_tag="AC16G0_00745"
                     /inference="COORDINATES: protein motif:HMM:NF045702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraether lipid synthase Tes"
                     /protein_id="HIR5098177.1"
                     /translation="MITLEGRQIPPITRSLPRTVRSLCPECHQTIPADEFIEDGRVIM
                     QKTCPAHGTFRDIIFSDADMFLRMEEWHFGDGQGFVNPAGDPEGECPHRCGICSWHTS
                     HTSLANVDLTSRCNLACHICFADANQHPYELSFEQAQETLRRLRAQRPAPAFAVQFTG
                     GEPTVHPRFLDILASAKAEGFSHIQVASNGLALADPDFACRARDAGLQYIYLQMDGTS
                     DDVFRKIRGRALLDKKLAVLQAARKAGLRVIFVPTILKGVNDHEIGDLFRLAFEHLDV
                     LSGISFQPMTFTGRMPEGDRLRLRFTLSDFAREFSGQTGITHPQEDWFPLSSAVPLIR
                     YVGALSGAALVNHTCHPHCGLMTLLFVDQDRRATPITQFLDLYRLLRDLEGLTPKAQK
                     TRVKALSKLQALQALNRFFKSERAPEGLTFLRFLQTLDGFADKKYSWDDRYRGHTYKT
                     FYLAGMHFMDAYNYDLERVARCGVHYSAPDGRIYPFCSYNSGPTYRNRVEGEFGCA"
     gene            complement(5084..6031)
                     /locus_tag="AC16G0_00750"
     CDS             complement(5084..6031)
                     /locus_tag="AC16G0_00750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR5098178.1"
                     /translation="MGTPVPDGLARHLNREVIVETMADTFARQKGYRHLLSVPSFYRL
                     GFAAAQCIPASVLYGVADLLGMATYLICRAPGTIVRANLVRAFPNASDREVSRLTAKI
                     FRNYARYLVDYGRYRWVPRADSQTVIPVLEGRENLDAAFKIGRGVILVTGHVGNWELG
                     GVSFGLRGIKVNVVTLPDGSRQIDAIRKGYRGQYSINTIVLDDSPFASLEMMAALKRG
                     EVVAMLVDRWGRSDGVLAPFFGGVHCLPRGPFVLSRVTGAPILPAFVVRDGRSYRGIL
                     ESPFVVEEDGLRPYALRVSNALEAVIRRYPDQWYNFVPL"
     gene            complement(6042..>6748)
                     /locus_tag="AC16G0_00755"
     CDS             complement(6042..>6748)
                     /locus_tag="AC16G0_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5098179.1"
                     /translation="RSLEIAKALRSRGVSKRWLLVQTRTDIICREPGLLEAWRPLAKD
                     FDIFFGFEAANDAGLAQVNKDAGVSSSLEAVRIARSLGYGVNGNFLVDPEWTEADFRE
                     LWDFVAQHGLRRAGFTILTPLPGTQFFQGLAPRLAGQPWFKYDMHHLLWEPRLGARRF
                     FELYAETWRRSILNASGDKKWLDWMRQVRPAKIPYLTSVLWRTQRMMKPEAYLREYEA
                     TQVMCDKVGREAPASP"
BASE COUNT         1302 a         2137 c         2136 g         1173 t
ORIGIN      
        1 atcggcgaag ggaatccgga ccagcttttc caggcgctcg cggttcctcg tgacgagcaa
       61 ttgtgtggag agctccatgg ggaagagcac gtcatactgg cccgtggcca gttcccgctc
      121 caaggccgcc aggaaatcct tcggctgtct ggcgacggag ggatagacga gcttgcgacg
      181 gcagtaccgt gagaacgcgg ctggagcgaa ccgggtcgtc tcccccacgg cggcaaagaa
      241 accccgccgg cccagcgacc gcacagccgc cagggtcttg ttccagaagc cgtccagtat
      301 cagcactcga ggagccatgc cgcaccgcct gggcgtcagg aggcttgcag ggtgtccccc
      361 acgaccgatt cgaccggccg gaattccccc gcgcgagccg cgagcttctt gatcgagcga
      421 cggaaaccgt aattcatggg cagccagaag ttcgccttgg aggttgtgcc ccaaaaccgc
      481 tggaggatgg cgggtacggt gtgcaccgcc atctgcaacc ggtaataccc gtcgaacagt
      541 tcccgtgccg tcattcccat cggccggtac accacattgt tggtgttata gttcgaccag
      601 tcgagatcga agattcggcc ctcaccgtag agcttctggt gcaaacgcgt tccggggtag
      661 ggggtgagga tggagaaata cggactctcc agcttggcct tctgcacgaa ctcgtaggtc
      721 cggtcgatga cccccggatc gtcgccgtcc aggccgaaga cgaacgcgcc gctgacgccg
      781 atcccgaaat cgtggatctt gtggatcgcg tccatgtagc cttcagggct gttgaagccc
      841 ttccgcaccg ccttgagtcg ctccttggaa agcgtctcga agccgatcaa gaggcccatg
      901 cagccgctct tccggcacag ctccagcaat tcgtcgtctc tggcgatggt agtggaggcc
      961 tggccgaccc atttgagctg gtaggggatg atgcgcgtca acaggtcgcg ggcgtgggta
     1021 cgctttccca cgatgttgtc gtcaacgaag aagaccacat tcttcagggt gaaccgccct
     1081 tcgaatggac ggagcccccg gatctcttgt tccacctctt ccggcggtcg ggttcggtaa
     1141 catcccccga agtaattcgt gacggaacaa aagtcgcatc cgtgcgggca cccgcgactg
     1201 gtctccacgc agtggaacgg caggtaccgc ttcccgtcgt agagggacca gcgagggctg
     1261 ccgagctgcg agaggggggg gaaatcctga tggcggtagc agcgctgcag ggcaccctgc
     1321 cgaaagtctg aaaggaggcg cggccagagg tcttccgcct cgccgacgac cacggcatcg
     1381 gaatgcgcca gcgcctcctc cggcagcatg ctgacgtgga tcccgcccat gaccacgggc
     1441 acgccgcggg cgcggaaggt gtcggcgatc tcgtatgccc ggttgatggc cggggtcatg
     1501 cccgtgattc cgacgaggtc caccgaacgc gagaaatcta tcggctccac cttctcgtcc
     1561 aggatggtga cggtccagtc gggaggggtc gcccccgcga cccggaggag gcccagggtg
     1621 ggcatccgga agtaaaaatc ggcccccaac aggctcttgg aagtgagagg agagacgagc
     1681 aggaggttca tgcggcctcc cgtccgaccg tgtccccgtg catgagcttc cggtacacct
     1741 tgaagtagag gatcgaaccg gcctggcgca ggattgcgct cacgggatgc ccgtcgccca
     1801 ggcactgccg gcaataggga tgaatcacac ggtaccttcg aaaaccctca gcaatctcct
     1861 ggaccgcggg gagcgagaga tagtcccgca gcggggtctg ggagcagttc gccaagaccg
     1921 tcttgccatc gtagtccgca cagcagatca cataatcccc gttccacagg attccgaaat
     1981 tctccgacaa cccggggcaa tacccaaacc gggcgggcac gttccggccc tcgaaatgtc
     2041 cggcccagtt cccgagggcg cggacccgaa agtccaggtc ctcggtcagc ggaacccggt
     2101 tttccttgag aatccccgca cccttgagcc gggccaggag ggcagggacc tgccgatcca
     2161 gcgccgtccc gctgaagatc cactccaccc agcgtgccat gtgggcgcgt aactcccttc
     2221 cggattccgc cgcccgaatg ccggatgcgc ctggggcgtg gaacctgcgc aggggattgg
     2281 acaggaagcc gatcgagagg tgcatccccg acggctggcc cagaaaggcg tgggtggtgc
     2341 tgatgaggcg ctcccggtac tgctcaaagg aaaggaggcc cgacccgcga cgcccgaagg
     2401 tcggcgcatc gggtgtctgg agggatatgc tgaggtggaa gaggccggcc gcttgaagtt
     2461 ccgacagcaa ctctggggtg aggaggcttc cattggtggt gacccacgcc tgcatgccgt
     2521 ggcggcgggc gagacggaca acctcgggca gagcggggta gcacagcggt tcccccatga
     2581 cgtggaagtg cacctgcctg gcgagaccct cctgccccgc ctgggcgagg aggcgttcca
     2641 ccatcgagag cggcatcgtc ccgcgaggcc ggcgcatgct ccgatcgggg cagaactcac
     2701 aggagaaatt gcaccgattg gtcagctcca ggtgtagccg ggggaccggg agctggatgg
     2761 ctccgatcct ggagaacatc ttaccggtca ctgccgccct cactggcaag cttgcccgca
     2821 tccgtcaccc ggacctcggg gcccagatgg agggtaaagg cgtcctcagg cacatgggga
     2881 ttgatgctca cgctcgagag cgtgagctcc gtacggtctc ccttctgccc gaggaccacg
     2941 atctgccggg gaatggcatc ggtagcgcgc tgggagatgg taaccgaagc caccgcttgc
     3001 gccacgaatc gggaccgggg ggtcagcctg ataaccaaga gctcatcgtc gcgatacagg
     3061 tccacctgga accgcttctc gaggtccacc accgccaggc tcatccccga cgtgaaaaaa
     3121 tccaccgcgg cgcgagcgcc gaaatcgtct ctcaggtccc tccgctcggc ctcccgtcta
     3181 tccgggtgat aggtaacaag ccagcggtca tcgatgagga tgatggtgcg gtcgggcttg
     3241 ttcacctccc accgcaccag attgggtctc ttgaagagga gcgttccctc gctcaccacc
     3301 tcggccgcca ggagcgggtg ccgcttcttc tggatgactg tggcgcgtac gctcgtgacg
     3361 gctcgctgtc gctcctggag cttctcgatg acctccaggc gttccttccc cagaatcggg
     3421 atttcggccg gcgccacatc ccccggccca agtggacccc aggaaaacag aaacgccgcg
     3481 agcacggcgg cccaaagaat aatgccggtg gggattcgga ctttctatct acgcgcatcc
     3541 gaattccccc tccacgcgat tccgatatgt cgggccggag ttgtacgaac aaaacgggta
     3601 gatccgtccg tcgggggccg agtagtggac tccgcaccgg gccacacgct ccaggtcgta
     3661 gttgtaggcg tccatgaaat gcattccggc caggtagaag gtcttgtagg tgtgcccccg
     3721 gtaccgatca tcccatgagt acttcttgtc ggcaaatcca tccagggtct ggaggaatcg
     3781 gaggaaggtc aaaccctccg gggcgcgctc cgatttgaag aaccgattca aggcttggag
     3841 ggcctgaagc ttggacaggg ccttgacacg ggtcttctgt gcctttggcg taagcccctc
     3901 aaggtctctc aggaggcgat acagatccaa gaactgcgtg atcggcgtgg cccgccggtc
     3961 ctggtccacg aaaagcaagg tcatgagacc gcagtgaggg tggcaggtgt gattgaccag
     4021 agcggctccc gagagcgcac cgacgtaccg gatcaggggc acggccgagc tcaagggaaa
     4081 ccaatcctcc tgggggtgcg tgatacccgt ttgaccgctg aactcccgcg cgaaatccga
     4141 gagggtgaag cgcaggcgga gccggtcccc ctcgggcatc cggccggtga acgtcatcgg
     4201 ctggaaggag ataccggaaa gcacatccag atgctcaaag gcgagccgga acagatcccc
     4261 gatctcgtgg tcattcaccc ccttgaggat ggtcgggacg aagatgaccc ggagccccgc
     4321 cttccgcgcc gcctgcagaa cggcgagctt cttgtccaag agggctctgc cgcggatctt
     4381 ccggaagacg tcatccgacg tcccgtccat ctggaggtag atgtactgca agcccgcatc
     4441 ccgggctcga caggcaaagt ccggatcggc gagtgccagc ccattgctgg ccacctggat
     4501 gtgggaaaaa ccctcagctt ttgccgaggc aaggatgtcc agaaagcggg gatgcaccgt
     4561 cggctcacct ccggtgaatt gcacggcaaa ggctggggcc ggccgctggg cacgaagcct
     4621 cctgagcgtc tcctgagcct gctcgaagga cagctcatag ggatgctggt tagcgtccgc
     4681 gaaacagatg tgacacgcca gattgcatcg ggacgtgagg tccacattgg ccagggaggt
     4741 gtgactggtg tgccagctac atatcccaca ccggtgcggg cattccccct cggggtcccc
     4801 tgcggggttc acgaatccct ggccgtcccc gaaatgccac tcctccatcc ggaggaacat
     4861 gtcggcgtcg gagaagatga tgtccctgaa ggttccatgg gccgggcagg tcttctgcat
     4921 gatcacccgc ccgtcctcga tgaactcgtc ggcagggatg gtctgatgac actccggaca
     4981 gagagaccgg accgtcctgg ggagtgacct ggtgatgggc gggatttgcc gtccctccag
     5041 cgttatcatg ggtgtgcccc acccttcctt tcggctcccg gtgctacagg ggaacaaaat
     5101 tgtaccactg atccgggtag cgtcggatca cggcctccag cgcgttgctg actcgcagcg
     5161 cgtaggggcg aagaccgtcc tcttcgacca caaatggaga ctccaggata cctcgatagg
     5221 agcgcccgtc gcgaacgacg aaggcgggca ggatcggcgc gccggtcacg cgactgagca
     5281 cgaagggtcc ccgggggagg cagtgtacgc ctccgaagaa gggggcgagg accccatccg
     5341 atctccccca tcggtccacg agcatggcaa ccacctcgcc ccgcttcagg gcggccatca
     5401 tttccaggga ggcgaagggc gagtcgtcaa gcacgatggt gttgatcgag tattggcccc
     5461 tgtacccctt ccggatggcg tcgatctgac gactgccatc cggcagcgtg acgacgttga
     5521 ctttgatgcc ccgaagaccg aaggataccc ctcccagttc ccagttgccg acgtggcccg
     5581 tgacgaggat cactcccctg cctatcttga aagccgcgtc caagttctcc cttccctcga
     5641 ggactgggat gacggtttgg gagtccgccc gtggcaccca tcgatatcgg ccgtagtcga
     5701 cgaggtacct cgcgtagttc cggaagatct tggccgtgag gcgggacacc tcccgatcgg
     5761 aggcgttcgg gaaggcccgc accaaattgg cacggacgat ggtacccggc gctcggcaga
     5821 tcagatacgt cgccattccc aggagatccg cgacgccgta caggaccgat gcgggtatgc
     5881 actgtgccgc cgcgaaccca agccggtaga acgagggaac ggagagcaag tgccgatagc
     5941 ccttctgtct cgcgaaagta tcggccatgg tttccacgat cacttcccgg ttcaggtgtc
     6001 gcgccagtcc atcagggact ggcgtcccca cccactccgc gctagggcga agcgggtgct
     6061 tccctcccga ccttgtcaca catcacctgc gtcgcctcgt actccctgag ataggcctcg
     6121 ggcttcatca tcctttgcgt ccgccacaag acgctggtga gataggggat cttcgccggt
     6181 cggacctgcc gcatccagtc cagccatttc ttatccccgg acgcattcag gatggaccgg
     6241 cgccacgtct cggcgtacag ctcgaagaag cgtcgtgccc cgaggcgggg ctcccagagc
     6301 agatggtgca tgtcgtactt gaaccacggt tggcccgcga gtcgaggagc gagtccctgg
     6361 aagaactggg tgcccggcaa gggtgtgagg atcgtgaacc cggctcgccg caggccatgc
     6421 tgcgcgacga aatcccacag ttccctgaag tccgcttctg tccactcggg atccaccaga
     6481 aagttcccgt tcaccccgta acccagcgac cgggcaatcc gcaccgcctc caggcttgac
     6541 gagacccccg catccttgtt gacctgcgcc agaccggcgt cgttcgccgc ctcgaaacca
     6601 aaaaagatgt cgaagtcctt ggccaggggt cgccaggctt caagcaggcc gggctcacga
     6661 cagatgatgt cggtgcgggt ctgcaccagg agccaccgct tcgacacacc acggctgcgg
     6721 agcgccttgg ctatctctag gctgcgcg
//
LOCUS       DBNGZC010000042         2969 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1763915, whole genome shotgun sequence.
ACCESSION   DBNGZC010000042 DBNGZC010000000
VERSION     DBNGZC010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1763915"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="AC16G0_00760"
     CDS             complement(<1..271)
                     /locus_tag="AC16G0_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5098180.1"
                     /translation="METPAIDVVENRFPISRNLTEFPFKSELSLAPLITTWSNGASGD
                     RQIAAMYHRQVLEEVRKAPELSAPIDDLSILDKYRDLVHVLMTRVF"
     gene            complement(427..>2969)
                     /locus_tag="AC16G0_00765"
     CDS             complement(427..>2969)
                     /locus_tag="AC16G0_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5098181.1"
                     /translation="QLDSVQAYRNLIVSTGNGAPVYLRDVAQVRDSVQDERINMRFWV
                     RGYPPPSATVVVAVNRQAGANAVEVAQRVRNLLPLVSAELPGSVRVTPIYDRSQTIVH
                     SVQDVETTLVIAFVLVEVVIFLFLGRATDTLIPSVALPLSLLLTFVAMRLLGYSLDNL
                     SLMALTLAIGFLVDDAIVFLENTVRRMEQGAGALEASVGSAKEISFTILSMTVSLAAV
                     FLPLVFMPGLVGRIFREFAITIVVAIFASGLVSLTLTPLMCARLLADRGPGTRRTWME
                     RVIGGAERRVLRMYGASLWWFLRRRWISALIWIVCLAGTVGLLLIVPKAFLPPGDSSF
                     IWGVMIAREGSSPAQMQALQDQADNVLRQEPGVGATFTMTGNGQFLSSNQGLLLAFLK
                     PHEERAPIQNVAGQLMGRLSAIPGVFPFLRPFPVLEISTGATNQNQGQYAFSLSGVNP
                     GQVYDVATKLMARLRGYPGFLTLSSDYFNNTPNLDIDIRREQARTYGVSETRILELLR
                     TAYAENYLYLIKKPEDQYQVILEVEDAARSKPEDLARLYIKSDDGKNLVPLSALVTWM
                     PSLGPQAVNHLNQFTSVTLFFNLKPGVAIGDATDFIRTAAAEIVPPTVRGSLQGEALT
                     FRDTVTNLTILMALAVFVMYVILAILYESYLHPLTVLSTLPTALVGGLLTLVVFGEQA
                     SLYAFVGMFMLMGIVKKNGIMIVDFARQRVAAGASAEQAIHDASLDRFRPILMTTLAA
                     VMGALPIAAGYGADGASRRPLGLVIVGGLVVSQFITLYITPVIYLYLERFQETVLDRT
                     SFLRSGYSRRPSEAGTAATVAPVPDMLTVAVGTPGDGLNGTGMATRTG"
BASE COUNT          549 a          939 c         1003 g          478 t
ORIGIN      
        1 ggaacaccct ggtcatcagg acgtggacga ggtcccgata cttgtcgagg atggaaagat
       61 cgtcgatggg cgcggacagt tccggcgcct tccggacctc ctccaggacc tggcggtggt
      121 acatcgcggc gatctgccga tcccccgagg cgccgttgct ccaggtggtg atcaggggag
      181 ctagactcag ctcgctcttg aaggggaact cggtaaggtt gcgactgatg ggaaatcggt
      241 tctcgaccac gtcaatagct ggcgtctcca tgcttcctcc accacaccgg gagcagtccg
      301 gcgagcttgt ccccggcgaa cgcctccagg cggctcgtgg ggattcacct gcaacttggc
      361 gattatacca ggggtactgc cgaaagacga gcatctccgt cctgaatgga ggcaacagcg
      421 cgccggtcaa ccggtcctcg ttgccatccc tgtgccgttc aggccgtcgc caggagttcc
      481 gacggcaacg gtaagcatgt cgggcacggg ggcaacggtt gcggcggtgc cagcctcgga
      541 ggggcgccgc gaatatcccg agcgcaggaa ggaagtgcgg tccagaaccg tctcctggaa
      601 ccgctccagg tacaggtaga tcacaggcgt gatgtacagg gtgatgaact gggacaccac
      661 caggcccccg acgatgacca gcccgagcgg ccggcgcgag gcgccgtccg ccccgtaacc
      721 tgctgcgatg ggaagcgcgc ccatgaccgc ggccagggtc gtcatgagga tggggcggaa
      781 gcggtccagg ctggcgtcgt ggatggcctg ttcggccgat gcaccggccg ccacccgctg
      841 acgagcgaaa tcgacgatca tgatcccgtt ctttttcacg atgcccatga gcatgaacat
      901 gcccacgaag gcatacagcg acgcttgctc gccgaacacc accagtgtga ggagcccccc
      961 cacgagcgca gtgggcaggg tcgacagcac cgtgaggggg tgcaggtagc tctcgtacag
     1021 gatggcgagg atcacgtaca tgacgaacac cgccagtgcc atcaggatgg tcaagttggt
     1081 gaccgtgtcg cggaacgtca gggcctctcc ttgcaggctc ccgcgcaccg tcggcggcac
     1141 gatctccgcc gccgccgtgc ggatgaagtc cgtggcgtcc ccgatggcca caccgggctt
     1201 gaggttgaag aagagcgtga cgctcgtgaa ctggttcagg tggttcacgg cctgggggcc
     1261 cagcgagggc atccaggtca ccagcgcgct gagcggcacg agattcttcc cgtcgtcgga
     1321 cttgatgtac aggcgtgcca ggtcttcggg cttcgagcgt gcggcgtctt cgacctccag
     1381 gatgacctgg tactggtcct cgggtttctt gatcaggtag aggtagttct ccgcgtacgc
     1441 ggtgcgtagc agctcgagga tccgagtctc ggacaccccg tacgtcctgg cctgctcccg
     1501 ccggatgtcg atgtcaaggt tgggcgtgtt gttgaaataa tcggaggaca gggtcaagaa
     1561 ccccgggtag ccccgcagtc gggccatcag cttcgtggcc acgtcgtata cctggcctgg
     1621 gttcaccccg gagagggaga aggcgtactg cccctggttc tggtttgtcg cccccgtgct
     1681 gatctccagc accgggaacg gccgcaggaa cgggaagacg ccgggaatgg cgctcagcct
     1741 gcccatcagc tggcctgcca cgttctggat gggcgcccgc tcctcgtgcg gcttcaggaa
     1801 ggcgagtagc agcccctggt tggaggacag gaattggccg ttccccgtca tcgtgaaggt
     1861 tgccccgacg ccgggttcct gccggagcac gttgtcggcc tggtcctgca gcgcctgcat
     1921 ctgcgcgggc gaggagccct cccgggcgat catgacgccc cagatgaagc tgctgtctcc
     1981 cggagggaga aaggctttgg ggacgattag gaggagcccg accgtgcccg cgagacagac
     2041 gatccagatc agggcggaga tccaccggcg tcgcaggaac caccagagcg acgcgccgta
     2101 catgcggagg acccgccgct ctgccccgcc gatcacccgt tccatccacg tccgccgggt
     2161 gccggggccc cggtccgcga gcagccgcgc gcacatgagc ggcgtgagcg tcagcgatac
     2221 caggccggaa gcgaagatgg cgaccacgat ggtgatggcg aactcgcgaa agatgcggcc
     2281 gaccagtccg ggcatgaaga ccagcgggag gaacaccgcc gccagcgaca cggtcatgga
     2341 caggatggtg aagctgatct ccttggcgct cccgacgctg gcctccagcg cccccgctcc
     2401 ctgctccatc cggcggaccg tgttttccag gaacacgatg gcgtcgtcca ccaggaaacc
     2461 gatggccagg gtgagcgcca tgagcgacag gttgtccagg ctatacccca agaggcgcat
     2521 cgccacgaag gtgaggagta gcgatagcgg cagcgccacc gatggaatca gagtatccgt
     2581 ggctcggccc aggaacagga agatcacgac ctccaccaag acgaaggcga tgaccagcgt
     2641 cgtctctacg tcctggaccg agtgcacgat ggtctgcgaa cgatcgtaga tcggggtgac
     2701 gcgcaccgac cccggcagct cggcgctgac cagcggcagg aggttccgaa cacgctgggc
     2761 cacctccacg gcgttggcgc ccgcctgccg attgaccgcc acgacgacgg tggccgaggg
     2821 cgggggatag cctcggaccc agaaccgcat attgatccgc tcgtcctgaa ccgagtcccg
     2881 cacctgggcc acgtcgcgga gatacaccgg ggcgccattg ccggtgctga cgatgagatt
     2941 gcggtacgcc tggacggagt cgagctggc
//
LOCUS       DBNGZC010000043         2252 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_296455, whole genome shotgun sequence.
ACCESSION   DBNGZC010000043 DBNGZC010000000
VERSION     DBNGZC010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_296455"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..823
                     /locus_tag="AC16G0_00770"
     CDS             26..823
                     /locus_tag="AC16G0_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098182.1"
                     /translation="MRGGAMRALILPVMTLWWREIVRFCRQRSRLTGAFLQPLVFWLL
                     LGGGLRASFRPPGTLSGTSYVEYTYPGIIAMVLLFTAIFATISVVEDRQQGFLQGVLV
                     APVPRASIVLGQAVGCTTLAVVQGVIFLLLAPLAGISLNVASAFWVTVVMVLVAFGLS
                     NLGLLIAWRLDSTQGFHAIMNLILLPMWVLSGAFFPAAGLPVWVEWTMRLNPLTYGMA
                     ALRRCLYLGNPAAAGAVPGLPLAVGVTIAFSIAAFVAATAATRDRAA"
     gene            1036..1467
                     /locus_tag="AC16G0_00775"
     CDS             1036..1467
                     /locus_tag="AC16G0_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098183.1"
                     /translation="MISGGTWILWVHLLSVALWLGGDAVLLGAILPVLGKEASVATAR
                     RAQFLTSRAMEVLVITGVLNLLVRGLASQMAFSRGFYAMLAVKMVLLFGMAGLQVWMG
                     FVWKRPDVDVATAARKARIGITLQLLFGAMAVLLGMGLHAA"
     gene            1844..>2252
                     /locus_tag="AC16G0_00780"
     CDS             1844..>2252
                     /locus_tag="AC16G0_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HIR5098184.1"
                     /translation="MKELVRPLKRGQWSESAMRVLRERYLDRSRRSPSDDAAGAARAG
                     EWVGETPEDMCWRVASAIAAAEATWGKTAEQIHEVASQFYELIVDGVFMPNSPTLMNA
                     GKDNGLQYSACFVLPVGDSMDDIFEAVKRAAIIH"
BASE COUNT          352 a          686 c          739 g          475 t
ORIGIN      
        1 accgcctctg gggcgaggag ggggcatgag gggaggagcg atgcgggccc tgatcctgcc
       61 ggtgatgaca ctctggtggc gcgagatcgt gcgtttttgc cgccagcgga gccgcctgac
      121 tggcgccttc ctacagcccc tggttttctg gctcctcctg ggaggggggc tgcgggcctc
      181 gttccggccg cccgggacgc tgtcgggaac gagctatgtg gagtacacct atcccggcat
      241 catcgccatg gtcctgctct tcaccgccat cttcgcgacc atctccgtgg tggaggatcg
      301 ccagcagggc ttcctgcagg gtgtgctggt ggcgcctgtg ccccgcgcga gcattgtgct
      361 aggacaggcg gtcggctgca ccacgctggc cgtggtccag ggcgtgatct ttctcctgtt
      421 ggcgcccctg gcagggatct ccctcaacgt ggcctcggcc ttctgggtga cggtcgtgat
      481 ggtgctggtg gcattcggat tgagcaacct ggggctgctc atcgcctggc gtctggattc
      541 gacccaaggc ttccatgcca tcatgaacct gatccttctc cccatgtggg tgctttccgg
      601 ggccttcttt cccgccgcag gactgcccgt gtgggtcgag tggaccatgc ggctgaaccc
      661 gctgacctat ggcatggcgg ccctgcgccg ctgcctctac ctgggaaacc ccgccgccgc
      721 cggggcggtg cccgggctgc cattggcggt gggcgtgacc atagccttca gcatcgcagc
      781 ttttgtcgcg gctactgccg cgacgcggga cagggcggcc tagcagtatg ctgaagaacc
      841 cgttttcgtt caggctgctc aaaaaggtcc agatgcaagg cggcaagccg ggtacccgtc
      901 cactaggatg ggtgcaggtg cgaggcgtac ttagtccgta cgccgcagcg ccgcgcgagc
      961 gcgccaacgc cgcagatggg cctttttcag cagcctgcta gccgaggtcg agtcaccctc
     1021 acaaccggag gcaagatgat cagcggaggc acgtggatcc tgtgggtgca cctcctgtcg
     1081 gtggccctct ggctgggcgg cgacgctgtt ctcctcggag cgatccttcc ggtattgggg
     1141 aaggaggcgt ctgtggccac ggccaggcga gcccaattcc ttacgtcgcg ggccatggag
     1201 gtcctggtca ttaccggcgt tctgaacctt ctggtccggg gactggcgag ccaaatggcg
     1261 ttcagccggg gattctacgc catgcttgcc gtcaagatgg tcctgctctt cgggatggcc
     1321 ggattgcagg tctggatggg tttcgtctgg aagcgtccgg atgtggacgt tgccaccgcc
     1381 gcccggaagg cccgcatcgg catcacgctc cagctccttt tcggcgccat ggcggtcctt
     1441 ctgggcatgg gcctccacgc ggcatagcct gcctgatacg ccgaaccccc tcagattccc
     1501 gtaatcattg aggcatgcgt agcctgtttc cgggcttcgc aagcaccttt ctcctgaacc
     1561 ctcaggtccc gaattttttc cttgacagtg ctatatcttg ggcatataaa ccaatcccac
     1621 ccaagatatg gaccaatcaa atgtttggct attataatca ctgcgtggaa aatctccatt
     1681 tggcggggag gtctaaggat ttcgtgcggt gcttccaggc agttgttctc atgattcgct
     1741 cctttcagtt gggcgacacg caggcttcgg cttgacctca gagaccgcct cgactgtctc
     1801 cgactggtcc gatgccgcgt ctagggtcta gggaggtttt tcgatgaaag agctcgtccg
     1861 accgttgaag cgtggccagt ggtccgaatc cgcgatgcga gtcctgcgag agcggtacct
     1921 ggaccgttcc agaagatctc cgagcgacga cgctgcgggt gccgcccggg cgggcgagtg
     1981 ggtcggtgag acgccggagg acatgtgctg gcgggtggcc agcgccatcg cggcggccga
     2041 ggcgacgtgg gggaagacgg cggagcagat ccacgaggtc gccagccagt tctacgagct
     2101 gatcgtggat ggggtcttca tgccgaactc cccgaccttg atgaacgccg gaaaggacaa
     2161 cggtcttcag tattcagcct gcttcgtcct gcccgtcggg gattccatgg acgatatctt
     2221 cgaggcggtg aagcgggccg ccatcatcca ca
//
LOCUS       DBNGZC010000044         4256 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1177325, whole genome shotgun sequence.
ACCESSION   DBNGZC010000044 DBNGZC010000000
VERSION     DBNGZC010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1177325"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="AC16G0_00785"
     CDS             <1..1081
                     /locus_tag="AC16G0_00785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5098185.1"
                     /translation="QAIQGLLAAGGRAKGVVQQILTFRRQTEQERKPVPVHLIVKEAL
                     KLLRASLPTTIEIRQQVDPEAGMVLADSTQIHQILMNLCTNAEHAMREKGGVLAVHLT
                     AVEVDPEFAASHPPLTPGPHVRLVVRDTGPGMEPRVRDRIFEPFFTTKGPGEGTGMGL
                     AVVHGIVAGHGGKITVDTAPGRGTKFEIYLPRCDVVQAVEMSPEEQARGRTDAPAPGQ
                     GERILIVDDEPYLAILWRVMLERLGYRVTGCSNSLEALEIFRAAPNSFDLVLTDQTMP
                     HMTGEALVKEVLRIRPDTPIILCTGFSHAMTEEKARALGIRAFLLKPISRRDLSLAIR
                     CVLDRRMPSVLQAAPGLVIVSSPTF"
     gene            1234..2304
                     /locus_tag="AC16G0_00790"
     CDS             1234..2304
                     /locus_tag="AC16G0_00790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014653.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HIR5098186.1"
                     /translation="MPGVDAGILFPADRLREWTQVVFQKVGVTREDAAILADSLIEAN
                     LRGVDTHGITRMLCVYVKRIQVGIMSPKTNLTVVRDHPSTALIDCKNSLGQVASAYAM
                     RKAIQKAETTGVAFVATTHSNHYGAAAYWAMMPLPHGMIGFSATNAPATVAPTGGRTA
                     MLGTNPFAMAIPAGSEKPMVLDLATTVVARGRILLYAKQNKPLEPGWAFDERGRPTVD
                     PQAALKGLLAPIGGYKGYGISLAVDLLCGVMTGSNYGAHFPGFLADNLKEPTDVGSIF
                     AALRVESFMDLSEFKGGMDKALREMKTSAKAEGVERIYIPGEIEFETKAERLAHGIPI
                     PEPVVQDFIALGKELGVPFPAD"
     gene            complement(2806..3798)
                     /locus_tag="AC16G0_00795"
     CDS             complement(2806..3798)
                     /locus_tag="AC16G0_00795"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5098187.1"
                     /translation="MPPLGLGLIGSGRIAQAHLKAAANLPSRVRAVSIAGRRLEQAEA
                     TARSFGIPNAVDDYRRLLSDPAVDAVLVATPNDSHASIVCEAAQAGKHILVEKPMALQ
                     AQSAEAMVRAAEAAGVTLMVAQSRRFSDAVRELVRRLPEIGEVFRIHITFLVSFPAPP
                     TDWWRSSTQAGGLVILLQGSHSLDSVCWWLNRTPSRVVATASRRNPAWEGEDEADILC
                     TFPAGEVASVHLSLSTSPALHEALVVGSLGHLRLIERAAGIPFENMYRLELDGQVLMD
                     APQTPSIYTHQLQEFADAVREKRAPLASGREILPVMRVLDAARASARTGLPVDL"
     gene            complement(3871..>4256)
                     /locus_tag="AC16G0_00800"
     CDS             complement(3871..>4256)
                     /locus_tag="AC16G0_00800"
                     /inference="COORDINATES: protein motif:HMM:NF014841.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5098188.1"
                     /translation="PLTAETRHLMNEKTLGLMKPTALLVNTARGGIVDTDALLRALSD
                     GRIGGAGLDTLEQEPISTDSPLLSLPNVLLAPHAGWYSDQSIIDLQRKTAEAAIAVLR
                     GQHPYSAVNPDVYATSKLRSKVPIL"
BASE COUNT          766 a         1375 c         1361 g          754 t
ORIGIN      
        1 gcaggcgatc caagggctcc tcgccgcggg cggacgggca aagggcgtgg tgcagcagat
       61 cctcaccttc cgccgccaga ccgagcagga gcgcaagccc gttccagtcc acctgatcgt
      121 caaggaggcg ctgaagcttc tgagggcatc tttgccgacg accatcgaga tccggcagca
      181 ggtcgatccc gaggccggga tggtcctggc cgattccacc cagattcacc agatcctgat
      241 gaacctttgc accaatgcgg aacacgcgat gcgtgagaag ggaggggtcc tggctgtgca
      301 cctgacggcg gtcgaggtgg atccggagtt cgcggcgagc cacccacccc tgacgcctgg
      361 accccacgtc agactggtcg tccgcgacac cggccccggc atggagccta gggtgcgaga
      421 ccggatattc gagcccttct tcacgaccaa gggtccgggc gaggggacgg ggatggggct
      481 cgcagtcgtc cacgggatcg tcgccgggca tggaggcaag atcacggtgg atactgctcc
      541 ggggcgagga accaagtttg agatctacct ccccaggtgc gatgtcgtcc aggcggtcga
      601 gatgtccccg gaggagcagg cccgtggcag gactgacgcg ccggcccctg gtcagggaga
      661 gcgcatcctc atcgtcgacg acgagccgta cctcgcgatc ctgtggcggg tgatgctgga
      721 gcgtctcggg taccgggtta ctgggtgctc caatagcctg gaagccctgg agattttccg
      781 cgcggcccca aactcctttg acttggtgct caccgatcag acgatgcccc acatgaccgg
      841 agaggcattg gtcaaagaag ttctgcgtat ccggcccgat acccccatca tcctctgcac
      901 cggcttcagc cacgccatga cggaggaaaa ggccagagcc ctcgggatcc gcgccttcct
      961 tctgaagccc atctctcgcc gcgatctcag cctggccatc cgatgcgtcc tggaccggcg
     1021 gatgccctcc gtacttcagg ccgcccccgg cctcgttatc gtctccagcc caaccttctg
     1081 atcccccgtc gccccccatg cttggccgca ccgttagcgg catccaggag gagttgtgct
     1141 acaatcggcc gtgacggtgc cagatctctc atcacggcca ccgcccgaga ctgcgcggtt
     1201 ccctcacagg cctcacagaa aggagcccga cccatgccag gtgttgacgc tggtatcttg
     1261 ttcccggctg accgactgcg cgagtggacc caggtggtct tccagaaggt cggggtgact
     1321 cgcgaggatg cagccattct cgccgactcc ctgatcgagg ccaacttgcg cggggtagac
     1381 acccacggca tcacccggat gctctgcgtc tacgtgaagc gcattcaggt gggaatcatg
     1441 agcccaaaga cgaacctgac ggtggtccgc gatcatcctt ccacggcgtt gatcgactgc
     1501 aagaacagct taggtcaagt cgcgtcggcc tatgccatgc ggaaggcgat ccagaaggcc
     1561 gagacgaccg gggtcgcctt cgtcgccaca acccactcca atcattatgg ggcggcggcg
     1621 tattgggcca tgatgccgct cccgcacggg atgatcggct tcagtgccac caacgcgcct
     1681 gcgactgtgg cccccaccgg tggccggact gccatgctcg ggaccaatcc gtttgccatg
     1741 gccatcccag cgggctctga gaagccgatg gtactcgacc tggccaccac ggtggtggcc
     1801 cgcggccgga ttctcctcta tgccaagcag aacaagccgt tggagccggg atgggcattc
     1861 gacgagcgcg gccggccgac cgtggaccca caggctgcct tgaagggcct gctggccccc
     1921 atcggcgggt acaaggggta cgggatttcc ctggcggtgg acctcttgtg cggggtcatg
     1981 accggctcga actacggagc ccacttcccc ggcttcctgg ccgacaacct gaaagagccc
     2041 acggatgtcg gcagcatctt tgcggctctc cgtgtcgaga gcttcatgga tctctccgag
     2101 ttcaagggcg gaatggacaa ggcattgcgc gagatgaaga cgtcggccaa ggctgaaggg
     2161 gtcgaacgga tctatatccc cggcgagatc gagttcgaga ccaaggccga gcggctggcc
     2221 catggcatcc cgatacccga gcccgtcgtc caggacttca tcgccctggg gaaggaactg
     2281 ggtgtgccct ttccggcaga ttgaaaaggc ggatcattca tccgcagata acgcagattg
     2341 acgcagattc ctgacggggt ctttcacgga tgcgatggcc tcgggcgtac ccccgaggca
     2401 attgcctttc tcggcgggtc tctgcgggaa gatcccggga taagtccaca tgacgccatg
     2461 ccgccaaccc gcgccgcagc ggcgccaggt tcagcggtga gttatgcgac gggttgcgtc
     2521 ttgttaacaa ggtcacggcc ggaggtcggc ggaggcagcg gcttgttgtc cttcctgtat
     2581 agggcaatcg tttcttcgac tatctgacaa agctcatcga agacccgctt ttcgtcatcc
     2641 ccatggcagc cgccgtagag caaacccggc gaacttccca cgtagcactg atcctcggcc
     2701 aatgtcaaac ctgacagaag gcgcgtctca gaggtcgctc atgtccttct ggagaatctg
     2761 cgtgatctgc gtgatctgcg gataattcag aggggctcgg ctccgctaca ggtccaccgg
     2821 aagccccgtc cgggcagagg cccgcgccgc atccagtacc cgcatgacgg ggaggatttc
     2881 ccggccggat gccagaggtg cgcgcttctc ccgaaccgca tctgcgaact cctgcaactg
     2941 gtgtgtgtat atgctgggcg tctgcggggc gtccatcaga acctgcccat ccagctccag
     3001 ccggtacatg ttctcgaacg ggatgccagc cgctcgctcg atgagcctga ggtggcccag
     3061 gctgccgacc accaaagcct cgtggagggc cgggctagtg ctgagggaca ggtggactga
     3121 tgccacctct cctgcgggaa aggtgcacag gatatccgcc tcgtcctcgc cttcccaggc
     3181 ggggttccgc ctgctggctg tggcgacgac gcgggacggg gttcggttga gccaccagca
     3241 gacgctgtcc agcgagtggc tgccctgaag taggatcacc agccccccgg cctgcgtgga
     3301 cgatcgccac cagtctgtgg gaggcgccgg gaaggagacc aggaacgtga tgtggatgcg
     3361 aaagacctcg ccaatctcgg gcagccgccg caccaactct cgcacggcgt cggagaagcg
     3421 ccggctctgt gccaccataa gcgtgacccc ggcggcctcc gcagcccgga ccatggcctc
     3481 ggcagattgg gcctggagcg ccatgggctt ttccaccagg atgtgcttcc ccgcctgcgc
     3541 cgcctcgcac acgatgctgg cgtgcgagtc attgggcgtg gcgacaagca cggcatccac
     3601 ggcgggatcg ctcagcaatc gccgataatc gtcaaccgcg ttcgggatcc cgaaggatcg
     3661 agccgtggct tccgcctgct cgagacgtcg gcctgcaatc gacaccgccc ggacccggct
     3721 cgggaggttg gctgcggcct tcaaatgggc ctgggcgatc cgaccactgc cgatgagacc
     3781 cagacctaga ggcggcattc ctttggacat tgccattctc cgtgagattg cgaggacctc
     3841 gacggcgccg gcacggcgct tgggcgcggg ctacaggatc ggcaccttgg agcgaagctt
     3901 cgatgtggcg tatacgtctg gattgaccgc cgagtatgga tgttgccctc ggagcaccgc
     3961 gatcgccgcc tcggccgtct tgcgctggag gtcgatgatg gattgatccg agtaccagcc
     4021 ggcatggggc gccaggagga cgttgggcaa cgagagcaac ggggaatccg tcgaaatcgg
     4081 ctcctgctcc agggtatcca ggcccgctcc cccgatgcgg ccgtctgata gcgcacgcag
     4141 caacgcgtcg gtgtccacga tcccgccccg cgcggtattc accaggaggg cggtcggctt
     4201 catcagcccc agagtctttt cattcatgag gtgccgtgtc tcggcggtga gcggca
//
LOCUS       DBNGZC010000045         1649 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2547286, whole genome shotgun sequence.
ACCESSION   DBNGZC010000045 DBNGZC010000000
VERSION     DBNGZC010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2547286"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..406)
                     /locus_tag="AC16G0_00805"
     CDS             complement(173..406)
                     /locus_tag="AC16G0_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098189.1"
                     /translation="MNVVAITPANVGRVARWRGERSGTRTYLQALMDGEWCQVVETRS
                     DPECLPPRRLRRMAGEFVWSPSRMSSGGSGRTG"
     gene            569..931
                     /locus_tag="AC16G0_00810"
     CDS             569..931
                     /locus_tag="AC16G0_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098190.1"
                     /translation="MAELRNAAGLNPDLVEELVKKHFGVEFTSKITWDFVKGGFAPSD
                     LPRRLATPQKVLGIRIGWKTFGEFTDNIGFRLELWDPAYLRQAEALVEEYNKKTEGKK
                     LELYPVSMYWPASARQAA"
     gene            1142..>1649
                     /locus_tag="AC16G0_00815"
     CDS             1142..>1649
                     /locus_tag="AC16G0_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR5098191.1"
                     /translation="MAILADQLRTVPYFRDLEPRVIERIRGRVFEVHLDKGQILFTEG
                     EPAEAMYVVRSGQVKICKLSPDGREQVLRIMGPGECFNEVPIFDEGPNPANAQALEPA
                     LVWGIRRADMRQLVAEHPAVAIGFLKVFAGKLRYFTRKVEDLSFRSVTSRVAKLLLEI
                     AEDDGRGGL"
BASE COUNT          321 a          550 c          491 g          287 t
ORIGIN      
        1 actcccactc ttgccgagct tgccggcaaa tcgtcgtgga gtctggatga gacacagtgc
       61 cgcctatcgc cctgccacgg cgatagcagg tcaagatcct tcaagcggcc ggcgtgagta
      121 tatgcaggag gttgtccgga atcaacagca ggcggtccaa tgtcagccaa tcctaacccg
      181 ttctcccgga gccgccggag gacatgcgtg agggagacca cacaaactct cccgccatcc
      241 gccgaaggcg tcgcggcggc agacattcgg ggtcgctcct cgtctcgacg acttggcacc
      301 actccccatc catcagggcc tgcaggtagg tccgggtgcc gctccgttct ccccgccagc
      361 gcgccacccg tcccacgttg gccggcgtga tcgccaccac gttcattgga atgccttcct
      421 ccaccaaacc cttgcagatt ccttccgatt acggtaagaa tgcagaacac gcaggctggt
      481 cgccaatcgt caaaatgcca tgtgcccaca ggggagacgc ggcgaggcgt ccagccggct
      541 gagccaatca ccggaaaaga ggaggatgat ggccgagcta cgaaatgccg ccggactcaa
      601 cccggacctc gtcgaggagc tggtcaagaa gcacttcggg gtcgaattca cctcgaagat
      661 cacctgggac ttcgtcaagg gcggattcgc cccatcggat ttgccgcgac gtctcgcgac
      721 tccgcagaag gttcttggta tccggatcgg ctggaagacc ttcggcgagt tcaccgacaa
      781 catcggcttc cgactggaac tctgggatcc cgcctatctt cgtcaggccg aggccctggt
      841 cgaggaatac aacaagaaga ccgaggggaa gaaactcgaa ctctaccctg tctccatgta
      901 ctggcccgct tcggcaaggc aggcggcctg atcggagcca gcctccaggc cttggcaccc
      961 tccaccgcac agacacttca agtgccgacg ggtctgcgtg atccgcggcc ctgatattac
     1021 ccgcgcccgc tggaatgtca atcgggccat cggatctcgg cccggccgtc acgcggttga
     1081 cctgcctcgc ccatccccct agaatagcca tgctcgtgcc ccctgaggga gacgcaggcg
     1141 catggcgatt ctcgcggacc aactgcggac cgtcccttat ttccgggacc tggagccgcg
     1201 ggtgatagag cgcatccggg gaagagtgtt cgaggtccac ctggacaagg gtcagatcct
     1261 cttcacggag ggagagcctg ccgaggcgat gtacgtcgtc cggtccggcc aggtgaagat
     1321 ctgcaagctg tcccccgatg gacgggagca ggtcctccgc atcatgggcc ccggcgagtg
     1381 cttcaacgag gtccccatct tcgacgaagg ccccaaccct gccaacgccc aggccctgga
     1441 gcctgccctg gtctggggaa tccggcgtgc agacatgcgc caactcgtcg cggaacatcc
     1501 agccgtcgcc atcggcttcc tcaaggtgtt cgcgggaaaa ttgcgctact tcacgcgcaa
     1561 ggtcgaggac ctgtccttcc gcagcgtgac cagccgcgtg gctaagctcc tcctggaaat
     1621 cgcggaggat gatggccggg gcggcctcc
//
LOCUS       DBNGZC010000046         2880 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1177526, whole genome shotgun sequence.
ACCESSION   DBNGZC010000046 DBNGZC010000000
VERSION     DBNGZC010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1177526"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="AC16G0_00820"
     CDS             <1..774
                     /locus_tag="AC16G0_00820"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR5098192.1"
                     /translation="VAALAQAPDVKAVFATHSETSTGAVHDVQAIAAVVRRPPAILVV
                     DAITSLGVMDLPMDAWGVDIVVAGSQKALMLPPGLAFAALSDRAWARVPEARLPKFYF
                     NFAAERKSIEKNQSAYTPAVSLVVGLRESLRLILAEGLPNVFARHDRLARATRAGVQA
                     LGLELFPDHPGCACTAVTAPAGIDGGAIVKGFRTRGITIAGGQGSMKGKIFRIAHMGY
                     VDAFDVLTALGALELVLSDLGYPVKFGEGVRAAEQLLQK"
     gene            902..2509
                     /gene="serA"
                     /locus_tag="AC16G0_00825"
     CDS             902..2509
                     /gene="serA"
                     /locus_tag="AC16G0_00825"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIR5098193.1"
                     /translation="MTSQIDLPASRMRVLVTDNLAQKGVELLRQAPGIEVEVQPKLAP
                     DALKEHCQGKHALILRSATRVTADLLSSAQTLKVVGRAGVGVDNVDLEAATARGVVVM
                     NTPGGNTISAAEHTISMLLSLAKHIPQATASMKSGRWEKGKFLSVELSGKVLGIIGLG
                     RIGSEVAKRARAFNLRVLVYDPFVSAEAAESLGVELVDLPELYRQADFITIHTPLTPE
                     TTNLICAETIAQMKGGVRIVNCARGGIVNEADLCEALKSGKVAGAGLDVFAQEPITDS
                     PLFSLPNFIATPHLGAASEEAQEAVAVEIAQQVIDYLQKGIIRNAVNAPSVPLELLRR
                     LQPYLLLGEKLGRLASQLSEGRLAEIRLAYRGEISSLETTPLTVATVKGIRDPIHDRV
                     NMVNAMAIARGRGVRVVDSKSTEPTDFTSLITVGLHTDRGKSEVEGTLYHQDPRLVGI
                     DGLRLEAVLDGLLLVYSNMDVPGVIGRIGTMLGKNNVNIAGMQLGRQQRGGRAVSIVN
                     VDDPIPAPVLEEIRRMPNIVYAKLVRV"
     gene            2612..>2880
                     /locus_tag="AC16G0_00830"
     CDS             2612..>2880
                     /locus_tag="AC16G0_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR5098194.1"
                     /translation="MIPRTTIPKGVRVFTPAETAQRRVVEEKLLSVFQRWGFREIITP
                     TFEYLEVFEQPTGEAADDQIFKFVDRQTGRLLALRVDLTSQVARMV"
BASE COUNT          499 a          946 c          966 g          469 t
ORIGIN      
        1 gtcgcagccc tcgcccaagc gccggacgtg aaggccgtct tcgccaccca tagcgagacc
       61 tcgacgggag ctgtccacga cgtccaggcg attgccgccg tcgtccggcg acctcctgcc
      121 atcctggtgg tagatgccat caccagcctg ggtgtcatgg acctccccat ggacgcgtgg
      181 ggcgtggaca tcgtggtcgc cggatcgcag aaggcgttga tgctgccgcc tggcctggcc
      241 tttgccgccc tctcggacag ggcctgggcc cgcgtccccg aggccaggct gccgaagttc
      301 tacttcaact tcgccgccga gcgaaagtcc atcgagaaga accagagcgc ctatacccct
      361 gcggtgtctt tggtggtcgg cctgcgggag tctctgcgcc tcatcctcgc cgagggcctg
      421 cccaacgtct tcgccaggca tgaccggctg gctcgagcga cgcgcgccgg cgtgcaggcc
      481 ttgggtctgg agctgtttcc ggatcatcct gggtgtgcct gcacggcggt cacggcgccg
      541 gcgggcatcg acggcggtgc catcgtcaag ggatttcgca cccgcgggat cacgatcgcc
      601 ggtggtcagg ggagcatgaa gggcaagatc ttccgcatcg cccacatggg gtacgtggac
      661 gcattcgacg tcctgacggc cctgggggcg ctggaactcg tcctgagcga cctcggctat
      721 cccgtgaaat tcggcgaagg cgtacgagcc gcggagcaac tcctgcagaa gtagcccgtg
      781 acccgaccgc aactcaccgg caaggcgcca agaacgccaa gacgcgatcg gccgattccc
      841 gaatccttgg cgaccttggc gccttggcgg ttcataggtt tggtttggat gggggaccct
      901 catgaccagc cagattgact tgcccgcctc ccggatgcgg gtcctggtga ccgacaacct
      961 ggcccagaag ggggtggagc tgctccggca ggcacccggc atcgaggtgg aggtgcagcc
     1021 caagctcgcg cccgatgcct tgaaggagca ttgccagggg aagcacgccc tcatcctgcg
     1081 gagcgccacg cgggtcaccg ccgatctcct gtcgtcggcg cagacgctca aggtggtcgg
     1141 gcgggcgggg gtcggcgtgg acaacgtgga cctcgaggca gccacggccc ggggtgtggt
     1201 ggtgatgaac acgccgggag gcaataccat cagcgccgcc gagcatacga tctcgatgct
     1261 cctgagcctg gccaagcaca ttccccaggc cacggcctcc atgaagagcg ggagatggga
     1321 gaaggggaag ttcctcagcg tggagctgtc gggcaaggtc ctggggatca tcggtctcgg
     1381 tcgcatcggg tccgaggtgg ccaagcgggc cagggccttc aacctccgcg tgctggtcta
     1441 cgaccccttc gtcagcgccg aggcggccga atccctgggc gtggagctgg tggacctgcc
     1501 cgagctgtac cgtcaggcgg atttcatcac gatccacacg cccctgacgc cggaaaccac
     1561 gaacctgatc tgcgccgaga ccatcgccca gatgaagggc ggcgtccgga tcgtcaactg
     1621 cgcccggggc ggcatcgtga acgaggccga cctgtgcgag gcgttgaaga gcggcaaggt
     1681 ggcgggtgcc ggcctggacg ttttcgccca ggagcccatc accgatagcc ccctgttctc
     1741 cctcccgaat ttcatcgcca ccccgcacct gggcgccgcc agcgaggagg cgcaggaagc
     1801 ggtggcggtg gagatcgccc agcaggtgat cgactacctg cagaagggca tcatccggaa
     1861 tgcggtgaac gcgccctcgg tcccgctgga gctgctccgc agactccagc cctacctcct
     1921 gctgggcgaa aagctgggga gactggcctc ccagctctcc gaaggacggc tcgccgagat
     1981 ccgcctggcc tatcgtggcg agatctcctc cctggagacc acgccgctca ccgtggccac
     2041 ggtgaaaggg atccgggatc ccatccacga cagggtcaac atggtgaacg ccatggccat
     2101 cgccaggggc cggggcgtcc gcgtggtcga cagcaagtcc accgagccca ccgacttcac
     2161 cagcctgatc acggtcgggt tgcacacgga ccgcgggaag tcggaggtgg agggcacgct
     2221 ctaccaccag gatccccggc tcgtgggtat cgacgggctt cgcctggagg ccgtcctgga
     2281 cgggctgctg ctggtctatt cgaacatgga cgtacccgga gtcatcgggc ggatcggcac
     2341 gatgctcggc aagaacaacg tgaatatcgc cgggatgcag ctcgggcggc agcagcgcgg
     2401 cggccgcgcg gtctcgatcg tcaacgtgga cgatccgatt cccgcgccgg tgctggaaga
     2461 gatccggcgc atgcccaaca tcgtctacgc gaaactggtc cgtgtctgac cccgaagcga
     2521 ggttgaccgg ttcgaagctg gaggttcgac cttccggatg ttgaacctcg gaccttggac
     2581 cttgaacgtt gaaccgtgaa ccatcgagcc aatgatccca cgcaccacca ttcccaaagg
     2641 ggtccgcgtc ttcacaccgg ccgagacggc ccagcgccgg gtcgtggagg aaaagctcct
     2701 ctcggtgttc cagcgctggg gcttccggga gatcatcacc ccgaccttcg agtacctgga
     2761 ggtcttcgag cagccaacgg gggaggcggc agacgatcag atcttcaagt tcgtggatcg
     2821 gcagacgggc cgcctgctgg ccctccgcgt ggacctcacg tcccaggtgg cccgaatggt
//
LOCUS       DBNGZC010000047         2276 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2253378, whole genome shotgun sequence.
ACCESSION   DBNGZC010000047 DBNGZC010000000
VERSION     DBNGZC010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2253378"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="AC16G0_00835"
     CDS             complement(<1..640)
                     /locus_tag="AC16G0_00835"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098195.1"
                     /translation="MTSPQSVGSTIPPSHAVEGSGESRRRRWREREFWLQYGTLYAVI
                     ALVVVSSLVSPYFLTVENLFNVIRQWSMVALISVGMTFIIINRGIDVSGGSILALGCV
                     LGAAILPATGVGAMIVLVLLAGTLCGILNGVAITRGNIPPFITTLGMMTIARGLALII
                     AEGRTIPANLSSGFQFWFGRGRILNVPTPILLVVLVFLAAGFVLRMTKYGRHV"
     gene            complement(727..1710)
                     /locus_tag="AC16G0_00840"
     CDS             complement(727..1710)
                     /locus_tag="AC16G0_00840"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019124009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR5098196.1"
                     /translation="MREITYREAIREALREEMRRDERVIILGEDVADAGGVFKVTLGL
                     LAEFGPERVIDTPLSESAIVGAAVGASLMGMRPVAEIMFADFTTVAMDGLVNHAAKIH
                     YLYGGELSAPLVVRMAYGAGPKWGAQHTQSVESWLANVPGLTIVMPATPADAKGLLKA
                     AIRTPNPVVFLEHKLLYGKKGQVPDGEHIVPIGRADIKRPGDDVTIVASGMMLDRALA
                     AAATLAKEGTGAEVIDLRSVQPLDAETILASVEKTGRLVIVHEAPVHGGFGGEIAAVV
                     ANKALGFLDAPIQRVGGPWAPIPFGPILTDAYLPKEGDIIQAVRTTQSVAA"
     gene            complement(1707..>2276)
                     /locus_tag="AC16G0_00845"
     CDS             complement(1707..>2276)
                     /locus_tag="AC16G0_00845"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR5098197.1"
                     /translation="GDGAAQRGDFHEGLNFAGVFRLPAVFVLENNGFAEMTPVAKHFA
                     GLDFACRAQGYGFPGVRLDGNDIFAVYEAVQTAVARARAGDGPTLLECVTYRLRGHSE
                     SHLPTELREQAEIDQWRAKDPLPRMREHLLERGILSPDIVAKIGAETTEEIEDAVKFA
                     EESPSPDPGEIHRDVYAPEAPDLVVGRQA"
BASE COUNT          383 a          816 c          708 g          369 t
ORIGIN      
        1 ccacgtgccg gccgtacttg gtcatccgca agacaaaacc cgccgccagg aacaccagca
       61 ccacgagcag gatgggtgtg ggcacgttca ggattcgacc ccgtccaaac cagaactgaa
      121 agccggaaga cagattcgcg ggaatggtgc gcccttccgc gatgatcagc gccaggccgc
      181 gggcgatggt catcatcccc agggtcgtga tgaagggcgg gatgttcccc cgggtgatgg
      241 ccacgccgtt caggattccg cacagcgtgc cggccagcag caccagcacg atcatggccc
      301 ccaccccggt ggcgggcagg atggcggcgc ccaggacgca gcccagggcc aggatcgatc
      361 cccccgagac atcgatcccc cggttgatga tgatgaacgt catgcccacg ctgatcaggg
      421 ccaccatgga ccactggcgg atcacgttga agaggttttc gaccgtcagg aaatagggag
      481 acaccagcga agagaccacg accagggcga tgaccgcgta aagcgtgccg tactggagcc
      541 agaactcccg ttcccgccag cgccggcgcc gagactcccc ggacccttcc acggcgtgcg
      601 acggcgggat ggtcgacccg acactctggg gagaggtcat ctcgtccacc tcgggcccgt
      661 cgggcgtctt gcgtcttcgt gcctgagcct cccgcacttc atcggactgt gtcctctccc
      721 gttcgtctac gccgccacgc tctgcgtggt ccggaccgcc tggatgatgt ccccttcctt
      781 cggaagatac gcgtcggtca agatcggccc aaagggaatc ggcgcccagg gtccgcccac
      841 gcgctgaatg ggcgcgtcca ggaatccgag tgccttgttc gccaccaccg ccgcgatctc
      901 cccgccgaat cccccgtgga ccggggcctc gtggacgatc accagccggc cggtcttctc
      961 cacagaggcg aggatcgtct ccgcgtccag gggctggacc gaacggagat cgatcacctc
     1021 ggcgccggta ccttctttcg ccagggtcgc cgccgcggcc agcgcgcgat ccagcatcat
     1081 gccgctggcg acgatggtca catcgtcccc cggccgcttg atgtccgccc ggccgatcgg
     1141 gacgatgtgc tcgccgtcgg gcacctggcc tttcttgcca tacagcagct tgtgctccag
     1201 aaacaccacc gggttgggcg tgcggatcgc cgccttgagc aggcccttgg cgtctgcggg
     1261 agtcgccggc atcacgatgg tgaggcctgg cacgttcgcc agccaggact ccacgctctg
     1321 ggtgtgctgc gcaccccatt tcggcccggc gccgtaggcc atccggacca ccaggggtgc
     1381 cgacagctca ccgccgtaca ggtagtggat cttggcggcg tgattgacca gcccgtccat
     1441 cgcgacggtc gtgaagtcgg cgaacatgat ctcggcgacg ggacgcatcc ccatcaggga
     1501 ggctcccacg gccgccccga cgatcgccga ctcgctgagc ggcgtgtcga tgacccgctc
     1561 ggggccgaac tccgccagca gacccagggt caccttgaac actcccccgg catccgccac
     1621 gtcctccccc agaatgatga cccgttcatc gcggcgcatc tcctcgcgca gggcctcccg
     1681 tatggcctca cgataggtga tttccctcat gcctgcctcc ccaccaccag gtccggtgcc
     1741 tccggtgcgt agacgtcgcg gtgaatctcc cccggatcag gagaaggact ctcctcggcg
     1801 aacttcaccg catcctcgat ctcctccgtc gtctcggcgc caatcttggc cacgatgtcc
     1861 gggctgagga tgccgcgctc gagcagatgt tcccgcatcc gggggagggg gtccttggct
     1921 cgccactgat cgatctctgc ctgctcccgc agctccgtcg ggagatgact ctcggagtgt
     1981 ccgcgcaaac gatacgtcac gcactccagg agcgtcgggc catcgcctgc ccgggcacgg
     2041 gccacggccg tctgcaccgc ctcgtacacg gcgaagatgt cattgccgtc cagccggacg
     2101 ccggggaagc cgtacccctg ggcccggcag gcgaagtcca agccggcgaa gtgcttggcc
     2161 acgggcgtca tctcggcgaa accgttgttt tccaggacga agacggcggg gagcctgaag
     2221 acccccgcaa agttgagccc ctcgtgaaag tcgccacgct gggccgcccc gtcgcc
//
LOCUS       DBNGZC010000048         2461 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2155674, whole genome shotgun sequence.
ACCESSION   DBNGZC010000048 DBNGZC010000000
VERSION     DBNGZC010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2155674"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1069)
                     /locus_tag="AC16G0_00850"
     CDS             complement(113..1069)
                     /locus_tag="AC16G0_00850"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR5098198.1"
                     /translation="MRIHLTIPEKKALDVVGVGINVVDYLFRVPRFPQPDTKSDALAV
                     TTQGGGLTATAMVACRRLGLRARYIGKFGSDEVGHLAREMLAAEGLDLDGSVQAADAP
                     HRLSVVLVEEGTGHRTIVRHRDLRIWLQPEELSREAVCAGRLLHLDGYEGAAALQTAR
                     WAREAGIPVSVDAEDSTERREELFRLADILILSHGFGQELTGKTDVTDILRGLVRLGP
                     ACAGVTLGEKGSALLHRGQIVEVPGFRVDVVDTTGAGDVFHGAFLYGLLQGWEAEDIL
                     LFANAVSALKCTRLGGRAGIPRVAEVRAFLGARGEKKFLDPS"
     gene            complement(1148..1924)
                     /locus_tag="AC16G0_00855"
     CDS             complement(1148..1924)
                     /locus_tag="AC16G0_00855"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5098199.1"
                     /translation="MRLVGKSAIITGAGRGIGKATALRFAQEGADVLVPDLDLATSQQ
                     TAGEIKALGRKGLPMQVDLTRMADIQRMLETALREFGKIDVLVNNAGITQVRDPMQLT
                     EADWDRTLNLNLKAVFFCSQAVAREMIKRRTGVILNAASISGRSGKPMLVDYCASKFG
                     VIGLTQSLALALAKHGIRVNAVAPGIVDTDMWVSIDREWSALEGKPTGEMKRSRVAAI
                     PLGRIETPEDVAKLYTFLASDEADYITGQTYNVCGGLQLN"
     gene            complement(1997..>2461)
                     /locus_tag="AC16G0_00860"
     CDS             complement(1997..>2461)
                     /locus_tag="AC16G0_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5098200.1"
                     /translation="LIPWFILFTKMKMIDTYTALILTHLIVALPLVLWVMIGFFEDVP
                     GELIEAARIDGCSNFSAFLRIALPLVKPGIVATAILGFIFSWNNFLFSLIIAGFKTRT
                     LPIAVYNFLSYEEINWGGLTAAATIITFPVLILSLFIQKHIVRGLTFGAVKG"
BASE COUNT          439 a          798 c          802 g          422 t
ORIGIN      
        1 gccaccttga tctggacgtc gaaggggccg ctgggggtcg gaatcgggaa gtcttcgata
       61 tccagttgct cgatgccctt ccagaccgct gccttcatgg aatccgctcc tttcaggatg
      121 gatcgagaaa tttcttctcc ccgcgtgcgc cgagaaaggc ccgcacctct gccacgcggg
      181 gaataccggc gcgaccaccc aggcgggtgc acttcagcgc cgagactgcg ttggcgaaga
      241 ggagtatatc ctcggcctcc caaccctgca acaggccgta caggaatgcc ccatgaaaca
      301 cgtcacccgc gccggtggtg tccaccacat ccacccggaa gcccggcacc tcgacgatct
      361 gtccgcggtg gaggagggcg gagcccttct cgcccagggt cacacccgca caggcgggcc
      421 cgaggcgcac caagccgcgc aggatgtcgg tcacatccgt cttgccggtc agctcctggc
      481 cgaagccgtg gctcaggatg aggatatccg cgaggcggaa cagctcttcc ctccgctccg
      541 tggagtcctc cgcatccacc gagacgggaa tccccgcctc gcgggcccag cgggcggtct
      601 gaagggcggc ggccccctcg tagccgtcca ggtgcagcag gcgccccgcg cacaccgcct
      661 cgcgggatag ctcctctggc tggagccaga tgcgcaggtc gcggtggcga acgatggtcc
      721 ggtgacccgt cccctcctcc accaagacca cggagaggcg atggggtgcg tctgcagcct
      781 ggacggaccc gtcgagatcc aggccttcgg cggccagcat ctcgcgggcc aggtgtccga
      841 cctcgtcgct gccgaacttc ccgatgtacc gcgcgcggag acccaggcgc ctgcacgcca
      901 ccatggccgt ggcggtgaga ccgccgccct gggtcgtcac cgccagggcg tccgacttgg
      961 tgtcgggctg gggaaagcgg ggaacgcgga acagatagtc cacgacgttg atcccgacgc
     1021 cgacaacgtc caaggccttc ttctctggga tggtgagatg gatccgcatg gggtcccagc
     1081 ctgttcgacg ttcaggcttc ggggtcaaac gtccaatgtc caacgcccaa cgtcggatct
     1141 tgaatcttca gttcagttgc agtccgccgc acacgttgta ggtttggccc gtgatgtagt
     1201 ccgcctcgtc ggaggcgagg aacgtgtaga gcttggccac atcctcgggc gtctcgatcc
     1261 gtcccagggg aatggcggcg acgcggctgc gcttcatctc gccggtcggc ttgccctcca
     1321 gggccgacca ctcgcggtcg atgctcaccc acatgtcggt gtccacgatg ccgggcgcca
     1381 cggcgttgac ccggatgcca tgcttggcca gggccagggc caggctctgg gtgaggccga
     1441 tcaccccgaa cttggaagcg cagtaatcca ccagcatcgg ttttccggat cggccagaga
     1501 tggaggccgc gttcaggatg acccccgtcc ggcgcttgat catctcgcgg gccacggcct
     1561 gcgagcagaa gaagaccgcc ttcaagttga gattgagcgt gcgatcccag tcggcttccg
     1621 tgagctgcat ggggtcgcgg acctgggtga tgcccgcgtt gttcaccagg acgtcgatct
     1681 tcccgaactc ccgcagcgcg gtctccagca tgcgctggat gtccgccatg cgggtcagat
     1741 caacctgcat gggtagaccc ttccggccaa gggctttgat ctcccctgcg gtctgctggc
     1801 tggtcgcgag gtccaggtcc ggcaccagga cgtccgcgcc ctcttgcgcg aaccggaggg
     1861 cggtcgcctt cccgatgccg cggccggccc cggtgatgat ggccgatttt ccgacgagtc
     1921 gcatgacaag ctccttggat cggccactgg ccatgggcac aattcgcgat ttcggctaaa
     1981 ggcccagggc tcggcctcac cccttcacgg cgccgaaggt cagtccccgg acgatgtgct
     2041 tttgaatgaa cagggacagg atcaacacgg ggaaggtgat gatggtggcg gccgccgtaa
     2101 ggccgcccca gttgatctcc tcgtaggaga ggaaattgta cacggcgatg ggcagcgtgc
     2161 gcgtcttgaa gccggcaatg atcaaggaga acaggaagtt attccaggag aagatgaagc
     2221 caaggatggc cgtggccacg atgccgggct tcaccagcgg cagggcgatc cgcaggaagg
     2281 cggagaagtt ggagcagccg tcgattcggg ccgcttcgat cagctccccc ggcacgtcct
     2341 cgaagaagcc gatcatcacc cacaggacca ggggcagcgc cacgatgaga tgggtcaaga
     2401 tgagggcggt gtacgtatcg atcatcttca tcttggtgaa caggatgaac cacgggatca
     2461 g
//
LOCUS       DBNGZC010000049         2806 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_981933, whole genome shotgun sequence.
ACCESSION   DBNGZC010000049 DBNGZC010000000
VERSION     DBNGZC010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_981933"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="AC16G0_00865"
     CDS             complement(<1..833)
                     /locus_tag="AC16G0_00865"
                     /inference="COORDINATES: protein motif:HMM:NF025843.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR5098201.1"
                     /translation="MARITYVSEESQYVVARLDVPGKPDPVTIVGTVLSLTPGETLRV
                     HGRWSHHPKYGEQFQVDRYESIVPASINGIRKYLGSGMIKGIGPVFAGRLVETFGEET
                     LRVIEEAPTRLAEVEGIGPKRQQRITAAWAEQREIREVMLFLQGLGVSPAYAVKIFKM
                     YGQAAIPTVRENPYRLARDIRGIGFKTADKIARELGIPADSPLRAAAGILHTLNELTD
                     EGHVYVPETELLRAAETRLEIPAGLLPDALAALSADEAVVVEPLPSGRAVYPTGLHVA
                     ET"
     gene            complement(1288..1542)
                     /locus_tag="AC16G0_00870"
     CDS             complement(1288..1542)
                     /locus_tag="AC16G0_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098202.1"
                     /translation="MERHQDDRSPDRLSDEELEYYGDLYIQCKIKESGLDFESFLGKP
                     EYYLRKYAQGRWDDGRDNEKGRKGFLRYLRFRPVARMPSE"
     gene            1884..>2806
                     /locus_tag="AC16G0_00875"
     CDS             1884..>2806
                     /locus_tag="AC16G0_00875"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5098203.1"
                     /translation="MAGRSGSEVVAVPPEDLLRIPEGSKLFTMPGSQPVAWDGRRHAF
                     HPAERVRIGGRSFACTTVAAFMPPGYTRTLLPAAFYPSPQPPLPLWPYTAVGWDGEDF
                     VAASIRTDPMDHSAARHYDDRELLPLIQERLRRAPENPILHQLVRCATEYHCLAAKNL
                     FLERWEAPLPVANSCNSACVGCISWQPSTACQASHERIRYMPTAEDVVEVALPFLTAV
                     PEAIASFGQGCEGEPLLRADVIAEALRRLRASTDRGTLNCNTNGSLPRRVEQIAQAGL
                     DSIRISLNSALGPTYTAYYQPRFYRWEDVRES"
BASE COUNT          487 a          935 c          875 g          509 t
ORIGIN      
        1 gtctcggcca cgtgcagccc cgtgggatac accgcgcgtc ccgaggggag cggctccacg
       61 accaccgcct catcggcgct gagcgcggca agggcatcgg ggagaaggcc cgctgggatc
      121 tcgagcctcg tctcggccgc ccgcagcaac tccgtttccg gaacgtacac gtgcccttca
      181 tcggtgagct cgttgagtgt gtgcaggatg cctgcggcgg cgcggagcgg tgagtcagcg
      241 gggatgccca gctcccgagc gatcttgtcc gccgtcttga agccgattcc ccggatgtcc
      301 cgggcgaggc ggtagggatt ctcacggacg gtggggatgg cggcctgacc atacatcttg
      361 aagatcttca ctgcgtaggc cggggagacg ccgagtcctt gcaggaagag catcacctcc
      421 cggatctcgc gctgctccgc ccacgccgcg gtgatgcgct gctggcgttt cggaccgatg
      481 ccttcgacct cggcaagccg ggtgggtgcc tcctcgatga ccctgagcgt ctcctctccg
      541 aaggtctcga ccagtcgccc ggcgaagacg ggaccaatgc ccttgatcat gcccgagccg
      601 aggtattttc ggatgccgtt gatgctggcg gggacgatcg attcgtaccg gtcgacctgg
      661 aactgctcac catacttggg gtggtgggac cagcgaccat ggacgcggag cgtttccccc
      721 ggggtcaggc tcaggacggt gccgacgatg gtgacggggt cgggttttcc tgggacatcc
      781 aggcgagcca ccacgtactg cgactcctca ctcacgtacg tgatgcgagc caccgccccc
      841 tcgatcgtcg cgtacggctc ggcgcgaagt tggagatgat taccggtggc tgcaccagga
      901 tgaggactac gtggacgcat tcggctatcc gctcggacca agccgttcga gacccccgca
      961 cacactggag acgatcgacg ccctgggcct gccgtcgttt cgcctatgtg tcaccgtcat
     1021 tattgcctga atagcccgag gtgtcgagtt cgttagatgg caggaggcct cccatgtgcg
     1081 tccggatggc gtccggcagg atcgtctgat ccttggtaag attcgtggct ggagaggctg
     1141 gaacaggggt atgggaaagc cgaggcgtga gaggtgaaca ctgagacgta ccggacattc
     1201 gcggggcaga agaaactcac aggggatcaa cgagggcagg gctccgacgg tggtgccggg
     1261 cccgagccac ccacggggcc attcgaatca ctcggacggc atacgggcga ccgggcggaa
     1321 ccgtagatag cggaggaagc ccttccgccc tttctcgttg tcgcgcccgt cgtcccaacg
     1381 cccttgggcg tacttccgga ggtaatactc gggtttgccc aggaagctct cgaagtccag
     1441 accggattcc ttgatcttgc actggatgta gaggtcgccg taatactcaa gctcctcatc
     1501 cgaaagacgg tccggcgatc ggtcgtcttg gtgccgctcc atacccctcc tctacgtctt
     1561 tcccttctga acctcctgca atgcgagaat atagcacccc gatccgaggc cgacaacttc
     1621 tatcccggat gatccagaag cagaggagag attcctgcac ctcctagcga cagttgccgc
     1681 ttctcacctc tgaagctata ctgcaatccc agatgggatc ttgccggaaa ccacggaatc
     1741 cccggcacgc gcagataccg tcgaccagat ttcttccgtg aaatctgcgg aatgtgtgga
     1801 atctgtggac tagagaaagt ctcatgccga aactcgtcgt ggccgattcg tcaggacaga
     1861 tccttgacca tccttccctc gggatggcgg gccgctcggg gagcgaggtc gtcgccgttc
     1921 cccccgagga cttgctccgc atccctgagg gaagcaagct cttcaccatg ccaggaagtc
     1981 agccggtggc ctgggacggc cggcggcacg cattccaccc ggcggaacgg gtgcgcatcg
     2041 gcgggcggag cttcgcctgc accaccgtgg cagccttcat gccccccgga tacacgcgga
     2101 cgctattgcc ggcggcgttc tacccttccc cccagcctcc cctgcccctc tggccgtaca
     2161 cggccgtggg ctgggacggc gaggacttcg tggctgcctc cattcgtacg gatcccatgg
     2221 accactccgc ggcccggcac tacgacgatc gcgaactgct cccattgatc caggagcgcc
     2281 tgcgccgggc ccccgagaat ccgatcctgc atcagctcgt gcgctgtgcc accgagtacc
     2341 actgcctcgc ggccaagaac ctgttcctgg aacgctggga ggcgccgttg ccggtggcca
     2401 acagctgcaa ctccgcctgc gtgggctgca tctcttggca accctccaca gcctgccagg
     2461 cctcgcacga gcgcatccga tacatgccga ccgccgagga cgtggtcgag gtggccctgc
     2521 cgtttctcac tgccgttccc gaggccatcg ccagctttgg ccagggctgc gagggcgaac
     2581 ccctcctccg ggccgacgtg atcgccgagg ccctgcgccg gcttcgcgcc tcgacagacc
     2641 gcggcacgct caactgcaac acgaacggga gccttccccg gcgggtcgag cagatcgccc
     2701 aggccggctt ggacagcatc cgcatcagcc tgaacagcgc cctggggccc acctatactg
     2761 cctactacca accccgcttc taccgctggg aggacgtgcg ggaatc
//
LOCUS       DBNGZC010000050         4260 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2645015, whole genome shotgun sequence.
ACCESSION   DBNGZC010000050 DBNGZC010000000
VERSION     DBNGZC010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2645015"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="AC16G0_00880"
     CDS             <1..266
                     /locus_tag="AC16G0_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR5098204.1"
                     /translation="ADEPTGRPPTHTAAGTDLAVIAKAAGIPQAVSVTDEAAFSAAVD
                     RALAEPGPWFIAAKVMPEKATARPPRDPLFFKYRFMRAIEKAR"
     gene            531..908
                     /locus_tag="AC16G0_00885"
     CDS             531..908
                     /locus_tag="AC16G0_00885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR5098205.1"
                     /translation="MSEREQKAEALIEKMKKDRGYIYPEWEFAARSDPDFVEAYNNLY
                     RAALNDGQALDAKTRELVALGILAYRRDANAVKAHILRALRLGATTQELLEAIETTIV
                     PGGAPTFFVGLSALMQALNDTKG"
     gene            1111..2163
                     /locus_tag="AC16G0_00890"
     CDS             1111..2163
                     /locus_tag="AC16G0_00890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018210630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HIR5098206.1"
                     /translation="MAEKISVSVDKLRWHCQGVLTQVGLRETHAALVAESLLFADLRG
                     VSSHGVVRLSSYLDRVHAGVMRVDPEMKLVRDNPASGLLDACNGFGQVAGVKAMDIAI
                     KKAEDSGASIVAVKNSNHFGVAAFYAMRAVAARMIGVVLTNASPAMTPYNAKKRLLGT
                     NPLAVAIPAGSQKPIVLDMSTSVVARGKIRLVASRPGEKIPVGWAVDADGRPTDNPVE
                     ALKGSLTPIGGPKGAGLSLVIDLLCGALTGTALTGEVKNITDTSGPSRTGHMFVALNV
                     AKFIDAQTFSESIDSVIQGIKALPSADGTPVYLPGEIEFNLEARRRVEGIPLATDVVE
                     TLNRLGERHGVGKLLS"
     gene            2308..3204
                     /locus_tag="AC16G0_00895"
     CDS             2308..3204
                     /locus_tag="AC16G0_00895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR5098207.1"
                     /translation="MEIGCHLPMMQAPEATREALLTFAREAERRNIASLWVGDHIVLP
                     RVSEGYPGGGAFPRPPDTPYLEPVVALSAVAACTTRPRLGASVFILGHRHPVVMAKVL
                     ASLDAVSGGRLICGVGVGWWRQELDILGVPFHARGRQGDEILRVFKELWTAPNPAFEG
                     EFFRFRDIGFAPKPFQKPHPPIWVGGASAGAFRRVVTLGDGWHADSGSPAELRDGLTR
                     LRAVAEAAGRPFETITISLRFTLNDGLLAQGAQAVIDRLCEYKQLGVDHLLLVFRREE
                     LARMLEILALVTDTIRPAVDSA"
     gene            3238..>4260
                     /locus_tag="AC16G0_00900"
     CDS             3238..>4260
                     /locus_tag="AC16G0_00900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HIR5098208.1"
                     /translation="MPISPFPHLFSPFTLGNVTLKNRLVMAPMALNYSTEKGEVQQRQ
                     VDYYLERARGGIGLIITESNYISPEGRSERHRLGLYSDHMVPQHRKLVEAIHRENTPI
                     FVQLLHAGRNARAGAIGQYPVGPSAVPLLTKGQLYVGTIPRALTTPEIQQLVELHGKA
                     AWHGLSAGFDGIVVHASNGYLLHTFLSPRSNKRTDRYGGSEENRARLLLEVVRKIREV
                     IGPEMPMVVRVTAEEHQDGGYGLDFICRVAQWLEGAGVTEVNVSSGTHEEAEWTIPPR
                     TVPEGFNVENAARVKAAVRIAVSTVGRIKRPAMAEQILTEGKADLIWMGWALIADPHL
                     PEKAQEG"
BASE COUNT          779 a         1314 c         1419 g          748 t
ORIGIN      
        1 aggccgacga gcccaccggg cgcccgccga cccacaccgc cgccgggacc gacctggccg
       61 tcatcgccaa ggcggcgggg atcccccagg ccgtcagcgt gacggacgag gcagccttct
      121 cggcggccgt ggaccgagcc ctggccgaac ccggcccctg gttcatcgcc gccaaggtca
      181 tgcccgagaa ggccacggcc cgccccccgc gcgacccgct cttcttcaag taccgcttca
      241 tgcgggcgat cgagaaggcg cggtgagacg gatcttggcc gaagagggat cgggtcctcc
      301 tgccagccgg gatcggatga aggcagtccc cgtcagcgaa ccgtgatgcg aggtatggga
      361 caggggtcgc cggacaatgc cctcttgaga aagtgtgtct ggggctctct ttcgatcgtg
      421 gtgattcacg cccgggcggg ctctcagaag gatcatgtgc tccccaggca tgttgccagg
      481 accttgtagc atagctcata tcccactggt aacgggaagg gggggtaacc atgagcgagc
      541 gcgagcagaa ggcagaggcc ctgatcgaaa aaatgaagaa ggaccggggt tacatatacc
      601 ccgagtggga attcgccgct cgaagcgatc ccgacttcgt tgaagcctat aacaatctct
      661 atcgggcagc cctgaatgac ggccaggccc tggacgccaa gacccgagaa ctggtggccc
      721 tcggcatcct cgcgtaccgc cgggatgcca acgcggtcaa ggcacacatc ctccgggcgc
      781 tgcgcctggg ggccaccacc caggagcttt tagaggcgat cgagaccacc atcgtcccgg
      841 ggggcgcccc aaccttcttc gtcggcctga gtgccttgat gcaggccctc aacgacacca
      901 aggggtgagg ctggacccgg ccccgcaggg gaatacgggg tcctgtgccc gcggggcgcc
      961 ggtggtaggc tgcgcctgga cttggaccag cacgcagggg atgcccggca ttcctcgcct
     1021 tcggtcgaag atgtagtctg tattggcgcg gaggatacac accgagcgag gaacccaccg
     1081 cgcgaggcat ccactgcccg gagacggacg atggccgaga agatatcagt ttcggtggac
     1141 aagttgcgat ggcattgcca gggggtgtta actcaagtcg gcttgcgcga gacccacgcc
     1201 gccctggtgg ccgagtctct cttgttcgcc gatctccgcg gggtgagctc tcatggggtg
     1261 gtccgcctgt cctcctatct tgatcgggtc catgccggtg tgatgcgggt ggatccggag
     1321 atgaagctgg tgagggataa tcctgcgtcg ggattgcttg atgcgtgcaa cggcttcggg
     1381 caagttgccg gcgtgaaggc gatggacatt gcgatcaaga aggctgaaga ctccggggca
     1441 agtattgttg ccgtgaagaa ctcaaatcac tttggcgttg ccgcgttcta tgcgatgcgc
     1501 gccgtcgccg caaggatgat cggtgtggtg ctcaccaatg catcgcctgc aatgaccccc
     1561 tacaacgcga agaagcgact cctgggcacc aatcccctgg ctgtggcgat acccgccggc
     1621 tcacaaaagc cgatcgtgct cgatatgtcg acttcggtgg tggcgcgcgg gaagattcgc
     1681 cttgtagcat cccggccggg cgaaaagata ccggtggggt gggcggtcga tgccgatggg
     1741 agacctactg acaatccagt cgaggcgctg aaggggagtc ttacgccgat cggcgggccg
     1801 aagggagcag ggttgtcgct ggtcattgat ctgctttgcg gggcattgac cggcacggca
     1861 ttgacgggcg aggtgaagaa catcaccgat acctccgggc cctccagaac gggacatatg
     1921 ttcgttgccc tcaatgtcgc gaaattcatc gacgcgcaga cgttctcaga aagcatcgac
     1981 agcgtcattc aaggaatcaa ggcgctgcct tccgcggatg ggacgccggt ctatctccca
     2041 ggcgagatcg agttcaacct tgaggccaga cgtcgggtgg aaggtattcc gcttgcaacg
     2101 gatgtggttg aaactctcaa tcgtctcggc gaacgccatg gcgtgggaaa gcttttgtcc
     2161 tagagatgcc cgcgagcttg acgcgcggac tttcatctcc ccgtagacat tcatccggtc
     2221 cgcctccgcg cggaggaaaa cctgttccct ccaactgagc agccctgact cctggacccg
     2281 atccggtcag cacggaggaa tagacgaatg gagatcggct gccatcttcc catgatgcag
     2341 gcgcccgagg ccacgcgcga ggccctgctt acattcgcgc gtgaggcgga gcgccgcaac
     2401 atcgcatcgc tctgggtggg ggatcacatc gtccttccac gtgtatcgga gggctacccc
     2461 ggcggaggcg cattcccccg tcctccggac acaccgtacc tggagcccgt cgtcgccctt
     2521 tccgcagtcg ctgcctgcac gacccgaccg cggctgggtg cgtcggtatt catcctgggg
     2581 catcggcacc ccgtggtcat ggccaaggtg ctcgccagct tggacgccgt ttccgggggg
     2641 cgcctgatct gcggggtcgg cgtgggatgg tggagacagg aactggacat cctcggcgtc
     2701 ccctttcatg ctcgcggacg ccagggcgat gagatcctgc gcgtcttcaa ggagttgtgg
     2761 actgccccga accctgcctt tgagggcgag ttcttccgct tccgggacat cgggttcgcg
     2821 ccgaagccct tccagaagcc ccatccgccg atctgggtag gaggtgccag cgcgggggcg
     2881 ttccggcggg ttgtgacgct gggggacggc tggcacgcgg actccgggag tccggccgag
     2941 cttcgggacg ggctcacccg tctgcgagcg gtggcggaag cagccgggcg ccccttcgag
     3001 accatcacga tctcgctccg gttcacgctc aatgacggtc tcttggccca gggtgcccag
     3061 gcggtcatcg atcgcctgtg cgagtacaag caactcggtg tcgatcacct cctgctcgtg
     3121 ttccgccgtg aggagctcgc ccggatgctg gagatcctgg ctctggtaac ggataccatc
     3181 cgaccggcag tcgactctgc gtgaggcgcg aacgcgtccc gctggaaagg agaggccttg
     3241 ccgatttcgc ccttcccgca cctcttctcc cccttcacgc tgggcaatgt gacactcaag
     3301 aaccgcttgg tgatggcgcc catggcgctg aattactcga ccgagaaggg agaagtgcag
     3361 cagcggcagg tcgactacta cttggagcgg gcccgggggg gaatcgggct cattatcacg
     3421 gaatcgaact acatcagccc ggagggccgg agcgagcggc accgcctggg cctgtactcc
     3481 gaccacatgg tgcctcagca ccggaagctg gtggaggcga tccaccggga gaacacgccg
     3541 atattcgtgc aattgcttca cgccggccgc aatgcgcggg ctggcgccat cggccagtat
     3601 ccagtgggac cttcggccgt gccgctcctg accaaggggc aactctatgt gggcaccatc
     3661 ccccgggcgc tcacgacccc ggagatccag caactcgtcg agctgcacgg caaggccgcc
     3721 tggcacggcc tgtcggcggg gttcgatgga attgtggtcc acgcctccaa tggctatttg
     3781 cttcacacct tcctctcgcc ccgctccaac aagcggaccg accgctatgg cggcagcgaa
     3841 gaaaaccgag cccgcctctt gcttgaggtg gtgcgcaaga tccgtgaggt catcgggccg
     3901 gagatgccca tggtggtgcg ggtgaccgca gaagaacatc aggacggcgg ttacgggttg
     3961 gacttcatct gccgggtggc ccaatggttg gagggggccg gggtcaccga ggtaaacgtc
     4021 agttccggca cgcacgaaga ggccgagtgg accatccccc cgcggaccgt gccagaggga
     4081 ttcaacgtag agaacgcggc ccgagtcaag gcggcggtga ggatcgcggt cagcactgtg
     4141 ggccgcatca agcggccggc gatggccgag cagattctga cggagggcaa ggcagacctg
     4201 atctggatgg gatgggcgct gatcgccgat ccccatctgc cggaaaaggc gcaggagggg
//
LOCUS       DBNGZC010000051         1917 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1960563, whole genome shotgun sequence.
ACCESSION   DBNGZC010000051 DBNGZC010000000
VERSION     DBNGZC010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1960563"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /locus_tag="AC16G0_00905"
     CDS             complement(<1..1406)
                     /locus_tag="AC16G0_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002728364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR5098209.1"
                     /translation="MASDHAVRVPATSGRLLMSGNEAVARGALEAGVSVASSYPGTPA
                     TEILEALARYPDIYAEWAPNEKVAFEVAMGASLAGARSMVAMKHVGLNVAADPLMSGS
                     YIGVHGGFLIAVSDDVGMASSQNAQDTRYFARFAKVPLFEPSDSQEAKAFVPEALELS
                     EQHDTPVILRLTTRISHVKGLVDLRERPVARFLGFAKNPAKYVVLPAHARKRHPIVLQ
                     RLKDLALLAGASPLNRLEWGTRDIGIIASGPAYMYAREAFPNASFLKLGFSFPLPEQL
                     IREFAAHVERLFVIEELEGLVEADILAMGIRVDSREKLPRIGELTPQRLRQALVGPAP
                     KEVSSAAASAPPELERQPFPRPPTLCAGCPHMGVFYTLGKMKDLVITGDIGCYTLGAG
                     FPWNAIQTCVCMGASLGNALGIEKAYRMANQGVKVVAVIGDSTFLHSGMTGLLDIVYN
                     GGNVTVVILDNRTVGMTGG"
     gene            1655..1849
                     /locus_tag="AC16G0_00910"
     CDS             1655..1849
                     /locus_tag="AC16G0_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098210.1"
                     /translation="MAELIHVSRIRIVKDKGPVRRAYIENFPEPVRYGVHGGIKKFYG
                     VEPEEELPSTLDHVIAAVAG"
BASE COUNT          347 a          653 c          588 g          329 t
ORIGIN      
        1 ccacccgtca ttcccaccgt ccggttgtcc agaatgacca ccgtcacgtt gccgccgtta
       61 tacacgatgt ccagaaggcc cgtcatcccg gagtgcagga aggtggagtc gccgatgacc
      121 gccacgacct tgacgccctg gttggccatc cggtaggcct tctcgattcc cagggcattc
      181 cccaggctgg ctcccatgca cacgcatgtc tggatcgcat tccagggaaa gcctgccccc
      241 agcgtgtaac agccgatgtc gcccgtgatg accaggtcct tcatcttgcc cagggtgtag
      301 aacacgccca tgtgggggca accggcacac agcgtcggcg gtcgcgggaa cggctgtcgt
      361 tcgagctccg gcggtgccga ggctgcggca gaagagactt ccttcggcgc cgggccgacg
      421 agggcctgac gcagacgctg cggcgtcagc tctccgatgc gtgggagctt ctccctgctg
      481 tccacccgga tgcccatggc caggatgtcc gcctccacca agccctctag ctcctcgatc
      541 acgaacaggc gctccacgtg cgcagcgaat tctcggatga gctgctccgg cagcgggaac
      601 gagaagccca gcttgaggaa ggacgcgttc ggaaacgcct cgcgggcgta catgtacgcg
      661 gggccgctgg cgatgatccc gatgtctcgg gtgccccact ccagccggtt cagcggactc
      721 gcgcccgcca gcagcgcgag gtccttcaat cgctggagaa cgatggggtg cctcttccgc
      781 gcgtgcgcgg gcaggaccac gtacttggct ggattcttgg cgaagccgag gaatcgcgcc
      841 accggccgct cgcggagatc caccagcccc ttcacgtgac tgatgcgggt ggtgagccgc
      901 aggatgaccg gcgtgtcgtg ctgctcgctg agttccaggg cctcgggcac gaacgccttc
      961 gcctcctggc tgtcgcttgg ctcgaagagc ggcaccttgg cgaagcgggc gaagtagcgc
     1021 gtgtcctgcg cgttctggga gctggccatg ccaacgtcgt ccgagacggc gatcaggaat
     1081 cccccgtgca caccgatata ggagccgctc atcaagggat ccgccgccac gttcagcccc
     1141 acgtgcttca tggccaccat ggaccgcgcc cccgccaggg aggcgcccat ggcaacctcg
     1201 aaagctacct tctcgttggg cgcccactcg gcatatatgt ctggataccg cgccagggcc
     1261 tccaggatct ccgtggccgg tgtccccgga tagctcgagg cgacgctgac acccgcctcc
     1321 aaggcccctc gcgccaccgc ctcgttcccc gacatcagca gacgcccgga ggtcgcgggt
     1381 actctgaccg cgtgatcgct cgccatatcg ttatcctctc cctgtcgcca gaagggttcg
     1441 gggaatgggt tgatgaaaca agagcgagac agggtaacat ggattcactc aatacgaaag
     1501 cccgccggaa gcctccctgg acctttgttc gcatgggcta caaacgcatt ccgaccttgc
     1561 cccctttccg ccgagttggt gccgattgcc agcacatcgc aaatccggta cagtcctgca
     1621 acctttcaac ctgaaacaac caggaggagc acccatggcg gaactgatcc acgtctcacg
     1681 catccggatc gtcaaggaca aaggtccggt gcgccgcgcc tatatcgaga acttccccga
     1741 gcctgtgcgc tacggggtcc acggcggcat caagaagttc tacggggtgg agccggagga
     1801 ggagctgcca agcactctcg accacgtcat cgccgcggtc gcggggtgaa tgaccggcac
     1861 gctggccgtg gcgctggcgg caaggaagat cccgacacaa cccgaccgat tgtcctc
//
LOCUS       DBNGZC010000052         3010 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_101869, whole genome shotgun sequence.
ACCESSION   DBNGZC010000052 DBNGZC010000000
VERSION     DBNGZC010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_101869"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..640)
                     /locus_tag="AC16G0_00915"
     CDS             complement(38..640)
                     /locus_tag="AC16G0_00915"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR5098211.1"
                     /translation="MRRAVSHLKRVDPVLGEIIQRVGPCRIQYAEPDFETLARSIVFQ
                     QLNGKAARAIFARLKGALGNGSRMTPRETLSLSPEQMLALGLSRRKAEYLRDLAEKTE
                     AGQIEFSRLAALSDRDVIEHLTTVKGVGVWTAHMFLLFALRRPDILARGDFGIRSAIR
                     KAYRLRKLPTPARVEKLGAMWHPHSSLACWYLWRSTETVI"
     gene            complement(657..2015)
                     /locus_tag="AC16G0_00920"
     CDS             complement(657..2015)
                     /locus_tag="AC16G0_00920"
                     /inference="COORDINATES: protein motif:HMM:NF016354.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HIR5098212.1"
                     /translation="MQPLKIQAVEPGSPAAEAGVAPGAFLVSLNGQAVQDALGLRFLE
                     TARRVDLVWRDGDGAEHRARIQKPEDLSLGLDVDPLKMRACNNKCAFCFALQNARGMR
                     RALYFKDDDYRFSFLNGNFATLTNLSRADLDRIVEQRLSPLYISVHATDWALRNQILG
                     NPRAPNILEQIKGFAAARIFMHTQVVLCPGINDGEHLKQTLEDLSRFQPFVQTVALVP
                     VGLTQYREKLPVLRSPDRAYALSLLAWAEPWRRRFLEELGTRLAFPSDEFYLLAGRPF
                     PPGRTYEGYPQLGNGVGGCRKFLDEFGRLARKLPLSVSPARRFTVVTGVLAGPVIAGA
                     IQRLNRIGGLRVEMISVVNEFFGGSVTCAGLLTGTDVVKTLDRRRDQLGDAILIPSVA
                     FKDDEDIFLDDLPLRDLGTHLGRRALRVDASARGLVDAALTWTDAQSASSELPGSPLR
                     PT"
     gene            complement(2015..2548)
                     /locus_tag="AC16G0_00925"
     CDS             complement(2015..2548)
                     /locus_tag="AC16G0_00925"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR5098213.1"
                     /translation="MLTLANRLTIFRILMTPVITVLLLYRHVGLALALFILAAISDGL
                     DGFIARSRKEKTTLGMVLDPVADKLLLMSAVVTLTILKELPRWFAVILVSRDILLIGG
                     AVILYMFLGKVAMPPSWLGKITTGFQLVTVLVAMLDNFLPHIQLAILPLSVVTMLLTA
                     ASGVAYIIRGTRLLNDQ"
     gene            complement(2559..>3010)
                     /locus_tag="AC16G0_00930"
     CDS             complement(2559..>3010)
                     /locus_tag="AC16G0_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HIR5098214.1"
                     /translation="DQLFATLDPTLRRVVLPGDRVALLSDTVGFIRKIPHQLVAAFQA
                     TLEEIQEASVLLHVVDVSHPHAQLQQEAVEAVLGELGLADRPTILVYNKIDRLGQSPF
                     PWRAGSGRVATSAKTGAGLDDLRHEIVTCLDGAAIPAAAPAVEAFGG"
BASE COUNT          551 a          989 c         1000 g          470 t
ORIGIN      
        1 ggattcgccg gtctgggggg gggcccagga ctctgtctca gatcacagtc tcggtgctcc
       61 gccagaggta ccagcaggcc aggctggagt gggggtgcca catcgccccc aacttttcca
      121 ctctggccgg ggtgggcagt ttgcggagtc gataggcctt tcggatcgcc gaccggatgc
      181 cgaaatcccc gcgtgccagg atgtcgggcc ggcggagggc aaacaggagg aacatgtgcg
      241 cggtccacac cccgacgccc ttgacggtcg tgaggtgttc gatcacatcc ctatctgaga
      301 gcgcggccag ccgggagaac tcgatctgtc cggcttcggt cttctccgcg agatcgcgga
      361 ggtattccgc tttccgtcgg gacagaccga gcgccagcat ctgttcgggt gacagcgaga
      421 gggtttctcg cggcgtcatc cgcgacccgt ttcccagggc gcccttcaac cgggcgaaga
      481 tggcgcgggc ggccttgccg ttgagctgct ggaaaacgat gctccgcgcg agcgtctcga
      541 agtcgggctc ggcatactga atccgacaag gccccaccct ctggatgatc tcgccgagga
      601 caggatccac tctcttgaga tgagacacgg ctcgtcgcat gcggttcctc cggttgtcag
      661 gtcgggcgaa gcggggagcc tggcaactct gaggaggcag actgcgcatc cgtccaggtg
      721 agggctgcat cgaccaggcc tcgagcggag gcatccacac gcagggcacg acgcccaagg
      781 tgcgtcccca ggtcgcgcag cgggaggtca tccaggaaga tgtcctcgtc gtccttgaag
      841 gccaccgacg gaatgaggat cgcatcgcca agctggtccc gccgccggtc gagggtcttg
      901 acgacatccg tgccggtgag caggcccgcg cacgtcaccg agccgccgaa gaactcgttc
      961 accacggaga tcatctcgac acgcaggccg ccgatacgat tcagacgctg gatcgcaccc
     1021 gcaatgacag gaccagccag gacgcccgtg accacggtga agcggcgagc cggggaaacg
     1081 gagaggggga gcttccgtgc cagccgtcca aactcgtcca ggaacttccg gcagcccccg
     1141 acgccgttgc cgagctgtgg atatccctcg taggtgcggc ccggcgggaa cggcctgccg
     1201 gccaagaggt agaattcgtc ggagggaaag gccagccgcg tacccagctc ctccagaaag
     1261 cgccgccgcc acggctcggc ccaggccagg aggctcagcg cgtaggcccg atcgggcgag
     1321 cgaagcaccg ggagcttctc gcggtactgc gtcaacccca cggggacgag agccacggtc
     1381 tgcacaaatg gctggaaccg gctcagatcc tccagggtct gcttcaggtg ttccccatcg
     1441 ttgatccccg ggcacagcac cacctgggtg tgcatgaaga tccgggccgc cgcaaaccct
     1501 ttgatttgct ccaggatgtt cggggccctg gggttgccca ggatctggtt gcgcagtgcc
     1561 cagtcggtgg catggaccga gatgtagagc ggactcagac gctgctccac gatccggtcc
     1621 agatcggctc gggaaagatt cgtcagggtg gcgaagttgc cattcaggaa agagaaccgg
     1681 taatcgtcgt ccttgaagta gagggcccgg cgcatgcccc gggcattctg gagggcgaaa
     1741 cagaaggcgc acttgttgtt gcaggcccgc atcttgagcg ggtccacgtc caggcccagg
     1801 gacagatcct ccggcttctg gatgcgggct cgatgctccg ccccgtcccc gtcccgccag
     1861 acgaggtcca cccgacgggc cgtctccagg aatcggagcc ccagcgcgtc ctgaactgcc
     1921 tggccgttca gactcaccag aaaggccccc ggagccacgc cggcctccgc cgccgggctc
     1981 cccggctcca ctgcctgaat cttcaagggc tgcatcactg gtcgttcagg aggcgcgtgc
     2041 cccgaatgat gtaggccact cccgaggccg ccgtgaggag catcgtcaca acgctgagcg
     2101 gaaggatggc gagctggatg tggggcagga agttgtccaa catggcgacc agcaccgtga
     2161 ccagctgaaa gccggtggtg atcttgccca gccacgacgg gggcattgca acctttccca
     2221 ggaacatgta gaggatgacg gcaccgccga tcagcaggat atcccggctc accaggatga
     2281 ccgcgaacca ccgcggcagc tccttcagga tcgtcagcgt caccacggcc gacatcagga
     2341 ggagcttgtc tgccaccggg tccaggacca tgcccagggt ggtcttctcc ttcctggagc
     2401 gggcgatgaa cccgtccagg ccgtccgaga tcgccgccag aatgaacagc gccagggcca
     2461 gcccgacgtg ccggtacagg agcagcaccg tgatgaccgg ggtcatcagg atccggaaga
     2521 tcgtcaggcg gttcgcgagg gtcagcaccc ggccctcctc atcccccgaa ggcttccacg
     2581 gcaggggcgg ccgccgggat ggctgccccg tcgaggcacg tcacgatctc atgccggagg
     2641 tcgtccagcc cggcccccgt cttggccgag gtggcgacac gaccgctccc tgcgcgccag
     2701 gggaagggag actgtccgag acgatcgatc ttgttgtaca ccaggatcgt gggacgatcg
     2761 gccagcccca gctcccccaa gaccgcctcc accgcctcct gctggagctg ggcgtgaggg
     2821 tggctcacat ccaccacgtg gagcaggacg ctggcctcct ggatctcctc caaggtggct
     2881 tggaaggcgg ccacgagctg gtgcgggatc ttgcggatga aacccaccgt gtccgacagg
     2941 agcgccaccc ggtcgcccgg caggacgaca cggcggagcg tcggatccag cgtggcgaac
     3001 aactggtccc
//
LOCUS       DBNGZC010000053         1652 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2156180, whole genome shotgun sequence.
ACCESSION   DBNGZC010000053 DBNGZC010000000
VERSION     DBNGZC010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2156180"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="AC16G0_00935"
     CDS             <1..404
                     /locus_tag="AC16G0_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR5098215.1"
                     /translation="AKLGYRQVALGIISGWGGGPRLVHLLGRGRALRLMATGDLLSAE
                     EALAIGLVDQVVSPDQVMPSALALARRIAGNAPLAVRAYKRLVQLTVRVPLEAALEFE
                     TELFGPVWVSEDHDECVRAFFEKRQPNLLGR"
     gene            459..1643
                     /locus_tag="AC16G0_00940"
     CDS             459..1643
                     /locus_tag="AC16G0_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5098216.1"
                     /translation="MNRTFRWVLMAAFLFASAGVGLAWAGEVKPILVGTSVSLSGSYA
                     ASALYSLEGYKLWVEQVNKRGGLLGRPVKLIYYDDKSDANTGVQLYEKLITADKVDLI
                     VGPYSSGVTSAVSTVAEKHKMAMLGPEAADVKIYSRGYTYNFQGQTQAGKYMLGALVL
                     AKANGYKTLAMLAEDTAFPKAVSEEVAKIAGSYGLTVVFNETYPKGASDFSALLTKVK
                     QLKPDVIFANSYLPDSQGIIRQAREQEVNAKLFAVAVGAAEPEFGNLGSTADYVFGAT
                     QWAANMKTLGNAEFVQAYKQMFGRDPDYHSASNFGAMEVVEAAVKKAGSLDQDKIAAA
                     IASTEMDTVYGKFKVDGRGVQIGYVSALLQWQQGKQVVVWPETAALGKAMLPTPPWMA
                     RK"
BASE COUNT          308 a          477 c          592 g          275 t
ORIGIN      
        1 cggccaagct ggggtaccgg caggtcgcgc tcggcatcat cagcggctgg gggggcgggc
       61 ctcggctggt ccacctcttg ggcagaggcc gggcgctgcg gctgatggcg acgggcgatc
      121 tgttgtcggc ggaggaggcg ctggcaatcg gcctggtgga ccaggtggtc tcccccgacc
      181 aggtcatgcc ctcggctctg gccctggctc ggaggatcgc cgggaacgcg cccctggcag
      241 tccgggccta caagcgcctg gtgcaattga ctgtccgggt tccactggag gctgcgctgg
      301 agttcgagac ggagctgttc ggtcccgtgt gggtctcgga ggaccatgat gagtgcgtcc
      361 gagcgttctt cgaaaagcgc cagccgaacc tgctgggacg gtgatggggg tgaagtgacg
      421 ctcgatgaaa acttcgtgaa tgcagagggg gaggggaaat gaatcgaaca ttccggtggg
      481 tcctgatggc tgcgtttctg tttgcgtcgg caggtgtcgg cttggcgtgg gcgggcgagg
      541 tcaagccgat cctggtcggc acctctgtgt cgctcagcgg gagctatgcc gccagcgccc
      601 tgtactccct agaggggtac aagctctggg tggagcaggt caacaagcgg ggcggtctcc
      661 tgggtcggcc ggtcaagctg atctactacg acgacaagag cgacgccaac acgggcgtcc
      721 agctctacga gaagctgatc acggccgata aggtcgacct gatcgtgggg ccctattcca
      781 gcggcgtcac ctccgccgtg agcacggtcg cggagaagca caagatggcg atgctcgggc
      841 cggaagccgc cgacgtcaag atctacagtc ggggctacac gtacaacttc cagggccaga
      901 cccaggccgg aaagtacatg ctcggcgcct tggtgctggc caaggcgaac ggctacaaga
      961 ccctggcgat gctggcggag gacacggcgt ttcccaaggc cgtctcggag gaagtggcca
     1021 agatcgccgg gagctacggc ctgaccgtcg tcttcaacga gacctatccg aaaggcgcga
     1081 gcgacttcag tgccctgctg accaaggtga agcaactgaa gccggacgtg atcttcgcca
     1141 actcctatct gccggactcc caggggatca tccggcaggc ccgggagcaa gaggtgaacg
     1201 ccaagctctt cgcggtcgcc gtgggcgcgg cggagcccga gttcggcaac ctgggcagca
     1261 cggcggacta cgtctttggg gcgacccagt gggctgccaa catgaagacc ctaggcaatg
     1321 ccgagttcgt ccaggcctac aagcagatgt tcggaaggga tcctgattac cacagcgcgt
     1381 ccaacttcgg cgccatggag gttgtggagg ctgcggtcaa gaaggcgggc tcgctggatc
     1441 aggacaagat cgcggcagcc atcgcctcga cggagatgga cacggtgtac ggaaagttca
     1501 aggtcgacgg acgaggggtt cagatcggct acgtctcggc cctcctgcaa tggcagcaag
     1561 gcaagcaagt ggtcgtctgg cccgagacgg cggccctggg gaaggccatg ctgcccacgc
     1621 cgccgtggat ggcgcgcaag tagggggatt ca
//
LOCUS       DBNGZC010000054         3552 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_493323, whole genome shotgun sequence.
ACCESSION   DBNGZC010000054 DBNGZC010000000
VERSION     DBNGZC010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_493323"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /gene="eno"
                     /locus_tag="AC16G0_00945"
     CDS             <1..1183
                     /gene="eno"
                     /locus_tag="AC16G0_00945"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR5098217.1"
                     /translation="VLGRGASPCGTSVGQHEAFVLRDGGTRYAGLGVQRAVRNVVEVI
                     FPALKGRSVLDQRAIDQCLIVLDGTPGKSRLGANAIYSVSIAVARAASCALRVPLYRH
                     LGGPEACLLPVPMFNMINGGTFGDVQMEFQEFLVIPAAADRYSEALRVGVEIYSALGE
                     VIGHRYGKDRVRIGSSAGYGDPASEPAEVLEVLLEAERAAGYGGQCRLGLDCAASHFY
                     DPKAGCYRLGARAVGREELIASLENLARSYDLFMIEDPLEENDFDGFATITRRIPTLI
                     VGDDLFVTNLERVKQGVALRAANAMVLKPNMVGTISEALDAARFAQAHGYRLVGSGRA
                     GGSIDDPIPDIAVAVGAPLVKFGAPRTGERLGKQNCLLRIEEELGDSARFAGPEIFRV
                     S"
     gene            1293..2030
                     /locus_tag="AC16G0_00950"
     CDS             1293..2030
                     /locus_tag="AC16G0_00950"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR5098218.1"
                     /translation="MESRSGHPRPPRIGVSWVKAEQVATDPLVLAVRRAGGEPVPLMI
                     DAPSWTEQIQTLDGLVLSGGNAVDPRRYGQVNEGLCRTVIPQRDEREYEAFRFCRERS
                     LPVLGVCRGIQFLNVACGGSMLQDLPITTVLHEAVGETSRFHPIEVLAGTRLASIAGP
                     HKSLRVNSRHHQGLRLEHLAPGLRASALASDGVVEGLEGTGDPFLLGIQFHPERTGEV
                     PEMVAIFDVLVAQAKGQVPARGSSRAG"
     gene            2039..2992
                     /locus_tag="AC16G0_00955"
     CDS             2039..2992
                     /locus_tag="AC16G0_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098219.1"
                     /translation="MGQYILRRVLQAIPMLVGISFAAFVLLRLAPGGPMAVYAQNPNM
                     SEADMRRIEHILGLDQPIHIQYLKWAAGMATGNLGYSYRTGRPVGQIILERIPATFQL
                     MGCAYLIAISLGVTTGIVSAIRRHSLFDYCATTGAMIGLSVPTFWFGLVVIILFAAML
                     RWIPSGGIATLGAPFSIQDRLIHLVGPASVLGLWMTATWSRYTRSSILEVVGQDYIRT
                     ARAKGLRARAVLLRHTLRNALIPLITLAGLEFRNLFGGALVTETVFSWPGVGRLYLDS
                     LNYQDYSVILGLLLMTSVMVLVGSLLADIMYAVADPRIRLK"
     gene            3028..>3552
                     /locus_tag="AC16G0_00960"
     CDS             3028..>3552
                     /locus_tag="AC16G0_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024313.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098220.1"
                     /translation="MSAAAASPSASELKMLAGPSLFAAGARRNRSIVWAKFRRNRLAS
                     VSAVVVLLLILLAIFAPWIAPHDPLYIDMDHKFARPGEARFLLGADELGRDVLSRLLH
                     AGRVSLSVGLMTALITVLVGSLLGALAGYYGGVVDAVIMRLVDVLLSMPTIFLLLALA
                     AFLKPTVFTITLIIG"
BASE COUNT          547 a         1135 c         1228 g          642 t
ORIGIN      
        1 cgtgctgggg cggggcgcgt ctccctgcgg cacttccgtg ggccaacacg aggctttcgt
       61 cctgcgggac ggggggacgc gctacgctgg cctgggtgtc cagcgggccg tgcgaaacgt
      121 ggtcgaggtg atcttcccgg cgctcaaggg tcggagcgtc ctggaccagc gggccatcga
      181 ccagtgcctg atcgtgctgg acggaacacc gggcaaatca cgtcttgggg cgaacgcgat
      241 ctacagcgtg tccatcgccg tggctcgcgc ggcgtcctgc gccctgcggg taccgctcta
      301 ccgtcacctg ggcggacccg aggcatgcct ccttccggtc ccgatgttca acatgatcaa
      361 cggggggaca ttcggggacg tccagatgga gtttcaagag ttcctggtga ttccggcggc
      421 ggcggatcgg tattcagaag ccttgcgggt gggggtggag atctatagcg ccctgggcga
      481 ggtcatcggg caccggtacg gaaaggatcg cgtgcgcatc ggctcctcgg caggctatgg
      541 ggatccggcg agcgagcccg ccgaggtgct cgaggtattg ctggaggcgg agagggcggc
      601 ggggtacggc gggcagtgtc gcctcggcct ggactgcgcg gcgagccact tctacgaccc
      661 gaaagcgggc tgctaccgcc tcggagcgcg cgcggtaggt cgcgaagagc tcatcgcatc
      721 gctggaaaac ctggcccgct catacgacct gttcatgatc gaggatccgc tggaggagaa
      781 tgactttgac ggcttcgcga cgatcacccg ccggattccg accctgatcg tcggggacga
      841 cctgttcgtg acgaacctcg agcgggtgaa gcagggtgtg gctctccgcg cggcaaacgc
      901 catggtgctg aagccaaaca tggtgggaac gatttccgag gctctggatg ccgcccgatt
      961 tgcccaggcg cacgggtacc ggctcgtggg ctccgggcgt gccggcggga gcatcgacga
     1021 tccaatcccc gacatcgccg tggcggtggg cgcccccctg gtgaagttcg gggcgccgag
     1081 gacgggggag cggctgggga agcagaattg cctgctgcgg atcgaggaag aactgggcga
     1141 ctccgctcgc tttgccggtc cggagatctt cagggtaagc tgacagacaa tcgccggcct
     1201 ggacaggcga ggggcatctc gaatagactc cgctcgagcc caaggcaagc ggtcgagcga
     1261 ccccatcgct ggtctctggg agatcggaaa atatggaatc gagaagtggg cacccccggc
     1321 caccccgcat cggcgtgagc tgggtcaagg ccgagcaggt ggcgaccgat cccttggtcc
     1381 tggcggtccg aagggccggc ggtgagccag ttccgctgat gattgacgcg ccctcctgga
     1441 ccgagcaaat ccagacgctg gacggcctgg tcctgagcgg cgggaacgcg gtggacccgc
     1501 gccgctacgg gcaggtgaat gaggggttgt gccggaccgt cattccgcag cgcgacgaac
     1561 gggaatacga agcctttcgg ttctgccggg agcgcagtct accggtcctg ggtgtctgcc
     1621 gtgggatcca gttcctgaac gtggcctgcg gcggatccat gctgcaggat cttcccatca
     1681 cgaccgtcct gcacgaggcc gtcggcgaga cttcgcgctt tcaccccatc gaggtgttgg
     1741 cgggcacgcg gctggcatcg atcgcgggcc cccacaagag cctgcgggtg aacagccgcc
     1801 atcaccaggg gctccggctg gagcacctgg cgccagggct gcgggcttca gccctggcct
     1861 ccgatggcgt cgttgaggga ttggagggaa ccggcgatcc gttcctgctc gggatccagt
     1921 tccatcccga gcgtacgggc gaggtgcccg agatggtcgc catcttcgac gtgttggtgg
     1981 cgcaggccaa gggacaggtc ccggcaagag gatcgtcgag ggcggggtaa gggacggtat
     2041 gggacaatac atcctcaggc gagtgctgca ggccatcccc atgctggtgg ggatctcctt
     2101 cgcggccttc gtcctcctgc gcctggcgcc gggcggcccc atggcggtct acgcccagaa
     2161 tcccaacatg agcgaggccg acatgcggcg gatcgagcat atcctcggcc tggaccagcc
     2221 catccatatc cagtacctca aatgggccgc gggcatggcc acgggcaatt tggggtactc
     2281 ctaccgcacc ggccggcccg tggggcagat catcctggag cggatcccag ccaccttcca
     2341 gttgatgggc tgcgcctatc tgatcgcaat cagcctgggg gtcacgacgg gcatcgtaag
     2401 cgccatccgg cgccacagcc tcttcgacta ctgcgccacc accggggcga tgatcggcct
     2461 ctccgtcccg accttctggt tcgggctggt ggtcatcatc ctgtttgcgg ccatgctgcg
     2521 gtggatcccc tcgggcggca tcgccaccct gggggcaccc ttctcgattc aggaccggct
     2581 catccacctc gtcgggccgg cctcggtcct gggcctctgg atgaccgcca cctggagccg
     2641 atacacgcga tccagcatcc tggaggtcgt cggtcaggac tacatccgga ctgcccgggc
     2701 caaggggctc cgagcccggg ccgtgttgct gcgtcatacg ctccggaacg cgctgatccc
     2761 cctcatcaca ctggccgggt tggagttccg gaacctcttc ggcggggccc tggtgaccga
     2821 gaccgttttt tcctggccag gggtgggccg cctgtacctg gactccctga actaccagga
     2881 ctactcggtg atcctggggt tgctcctcat gacttcggtc atggtcctgg tggggagcct
     2941 cctggcggac atcatgtacg ccgtggcgga tcctcgcatt cgcttgaagt aggcggctgg
     3001 catgctttta cctcggaggg cccccagatg agcgcggcgg ctgcgagtcc gtccgcatcg
     3061 gagctgaaga tgctggcagg cccgtcgttg ttcgctgccg gggcccgacg gaaccggtcc
     3121 atcgtctggg ccaagttccg acgcaaccgg ctggcctctg tcagcgccgt ggtggtgttg
     3181 cttctgatac tgctggcgat ctttgccccg tggatcgccc cgcacgatcc gctgtacatc
     3241 gacatggatc acaagttcgc caggcccggc gaggccaggt tcctgctggg ggccgacgag
     3301 ctgggccgag acgtcctcag ccgcctcttg cacgcgggcc gcgtgtccct ctcggtcggg
     3361 ctgatgacgg ccctcatcac ggtcctagtg ggctccctcc tgggagccct ggcggggtac
     3421 tacggcgggg tcgtggacgc cgtcatcatg cgcctggtgg acgtgctgct gtccatgccg
     3481 accatcttcc tgctcctggc gctggcggca ttcctcaaac ccacggtgtt caccatcacc
     3541 ctcatcatcg gg
//
LOCUS       DBNGZC010000055         1937 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_102339, whole genome shotgun sequence.
ACCESSION   DBNGZC010000055 DBNGZC010000000
VERSION     DBNGZC010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_102339"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="AC16G0_00965"
     CDS             complement(<1..991)
                     /locus_tag="AC16G0_00965"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR5098221.1"
                     /translation="MKAWRFTWLGKLILLLLLAGAMENPALAQTQMGPSTDLSLGEAV
                     KLALEKHPALRAASHQAAAAATGVDQARAGFLPRVDFSEGMTRSNNPVYAFGSLLNQG
                     RFTQADFAVDNLNHPDPISNWRTNIGGSVPLFMGGRNLLGYQQAQLSQEAAERGRART
                     EQEIIYGVVRAYYAILLSQEARATVDASVRTAEANLAAAQARYESGVVVASDALAARV
                     RLARLKQEAIAAANQVALDRAALNDAIGVALDQSYRITGNLDLSPLRYERLEGVEALA
                     KEQRPDYRQAVLEEQRLEKEVLRTKGALLPTLHLMGNYEINNHQFASQGQDNWS"
     gene            complement(1206..1490)
                     /locus_tag="AC16G0_00970"
     CDS             complement(1206..1490)
                     /locus_tag="AC16G0_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR5098222.1"
                     /translation="MRHDKTSELTPHHEMSMSCRELFERLSEYVDGELPPEICEEIQR
                     HMDGCSPCVAFANTLKKTADLCRRLPAKPMPTEVADNLRAFIATYLSESK"
     gene            complement(1543..>1937)
                     /locus_tag="AC16G0_00975"
     CDS             complement(1543..>1937)
                     /locus_tag="AC16G0_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR5098223.1"
                     /translation="TWLYRIASNACLKKRRRGVHDPSPEQELSLDELMPRPDSEGRKP
                     EIADWSDDAERALLRGELSGQMEAAIDKLPKEYKIVLVLRDVEGFSAEETAEMLKLSI
                     PAVKSRLHRARVFVRRELAGYFQDQSKQ"
BASE COUNT          335 a          594 c          634 g          374 t
ORIGIN      
        1 cggaccagtt atcctgtccc tgggaggcga attggtggtt gttgatctcg tagttgccca
       61 tgaggtgcag cgttggcagc agggcgccct tggtgcggag cacctctttc tccagacgct
      121 gctcctccaa tacggcctgc cggtagtcgg ggcgctgctc cttcgccagg gcctccacac
      181 cctccagacg ctcgtagcgg agcggggaaa ggtccaggtt gccggtaatc cggtaggact
      241 gatccaaggc cacgccgatg gcatcgttca gcgcggcccg atccagcgcc acctgattcg
      301 ccgcggcgat ggcctcttgc ttcaggcggg cgagccggac ccgggcggcc agggcatccg
      361 acgccaccac gacgcctgac tcgtagcggg cctgggcggc tgccagattt gcttcggccg
      421 tccggaccga ggcatccacg gtggctcggg cctcctggct caggaggatg gcgtaatagg
      481 cccggacgac gccgtagatg atctcctgct ccgtgcgggc ccggccccgc tcggcggcct
      541 cctggctgag ctgcgcctgc tggtagccga gcagattgcg gccgcccatg aagagaggaa
      601 cgctcccccc gatatttgtt cgccagttgc tgatgggatc agggtgattc aggttgtcca
      661 cggcgaaatc tgcctgcgtg aagcgtccct ggttgaggag agagccgaag gcgtacaccg
      721 gattgttact ccgcgtcata ccttcggaga aatccacgcg ggggaggaag cctgcgcggg
      781 cctgatcaac ccccgtggcg gctgccgcgg cctgatggct cgcggcgcgg agggcagggt
      841 gcttttccag ggcgagcttc acggcttcgc cgagactgag atccgtgctc ggtcccatct
      901 gcgtctgggc caacgccgga ttctccatcg cccctgcgag gagtaggagc aggatcagct
      961 tgccgagcca ggtgaatcgc cacgctttca tcgggtcctc cgcgaagggg gcacatgtcg
     1021 gcctactgct ttcggtccag aggcagagat aaggattcac ggaaatcact cgacatgaga
     1081 tagccggata ccggagggga acaaccggcg tgtgcctatc atatacgtac cggcccaagc
     1141 gcttcaactc atcggaaggg atccggtccg acgtacaccg gtctcaaaag aggagggatg
     1201 tgggtttact tggactctga tagatacgtc gcgatgaagg cgcgcagatt gtcggcaacc
     1261 tccgtcggca tcggctttgc cgggaggcgc cggcacagat cggctgtttt cttcagggtg
     1321 ttggcaaagg ccacgcaggg ggagcacccg tccatgtgcc gctggatctc ctcacagatc
     1381 tctgggggga gctcaccatc cacatattcg gacaagcgct cgaacagttc gcgacagctc
     1441 atggacatct cgtggtgagg ggtcagttcg gaagtcttgt cgtgtctcat tgtctatgat
     1501 ccagaaagtt ggagttggat tcaaccggag catatcttgc tgtcattgct tggattggtc
     1561 ctggaaatac cccgccagct cgcgccggac gaagacgcgg gcgcggtgga ggcgggactt
     1621 caccgccggg atcgaaagct tcagcatctc ggcggtctct tccgcggaga agccttccac
     1681 gtcgcggaga accaggacga tcttgtactc cttgggcagc ttgtcgatgg ccgcctccat
     1741 ctgcccggac agctcgcctc gcagcaaagc ccgttcggcg tcgtcggacc agtcggcgat
     1801 ctcgggcttc cgtccctccg aatcgggacg gggcatcaac tcgtccaggg acaactcctg
     1861 ctccggcgag gggtcatgca cgccccgccg tcgcttcttc aggcaggcgt tggaggcgat
     1921 ccggtagagc cacgtcg
//
LOCUS       DBNGZC010000056         3518 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2645571, whole genome shotgun sequence.
ACCESSION   DBNGZC010000056 DBNGZC010000000
VERSION     DBNGZC010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2645571"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1441
                     /locus_tag="AC16G0_00980"
     CDS             <1..1441
                     /locus_tag="AC16G0_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR5098224.1"
                     /translation="GTFFGTIVGVLPGLGPTASIALLLPVTIGMDMVSALIMLAGIYY
                     GAMYGGSTTSILVNIPGEAASVVTCIDGYQMARQGRAGPALGMSAFGSFIGGTVSVVA
                     VMLISPILADTALKFGPPEMFALLFFAFTCIGGLTGKSKAQGLLMASVGLLLGSVGTD
                     EIHGVDRFVFGTLYLRDGIGLVPLAMGLFGLGEILFNIEQRETAGSVLETPKNLLPTL
                     RDWKDSAWPILRGSFLGFGIGVLPGGMPAVASLVRYAVEKRIARRPELFGKGAIQGVA
                     GPETANNAATAGGFVPLLTLGIPTNSVLALMLAALMLHGVTPGPLLLLKWPDVFWGVI
                     ASMYIGNVMLLVLNLPMIGLFVQLLKVPYGILSPLIIMFVLLGAYALDNSVADVLLTV
                     LFGLAGYLMKRFGLEPAPLVLAFVLGPMLENNLSKALIISRTGSPLFFFTRPISATLL
                     TLAILVLVYPLLRWCLRRGLGRSKRGAIA"
     gene            1540..2505
                     /locus_tag="AC16G0_00985"
     CDS             1540..2505
                     /locus_tag="AC16G0_00985"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HIR5098225.1"
                     /translation="MKPKVLVTETIHPAGWDRLRGEVEAVAWAGQERQPLALAVGDVQ
                     GILVRVAKLTGEVIQAAKQLKVIGKHGVGTDNIDIPAATAHGVLVTNTPLANSTSVAE
                     HALALLLGVARRIGESDRDLALGRLRSQRTYQGIELSGKVLGVIGLGSAGLRLAGMAG
                     QGLGMRLLGFDPYKAPWPEGVEHCGELASLLRQADFISIHVPLTKETRNLIDETALAA
                     MKPTAILVNTARGGIVDEAALAAALKTGGLAGAGLDVVVDEPLKPGHPLLGVPNLILT
                     PHMAGVTEEAMMRMALDSAEDILRVLRGEPPKYPVNREVLTPGRR"
     gene            2581..>3518
                     /locus_tag="AC16G0_00990"
     CDS             2581..>3518
                     /locus_tag="AC16G0_00990"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR5098226.1"
                     /translation="MKIVDVLANNLTIPYPGEVRPAWQPGLVATSHNFTLVRIRTDEG
                     ITGYAGTSGHAAGVISAQVKPYLVGEDPFATERLARVYRSAGNGVWCIDMALWDIIGK
                     AANLPLHKLWGSYSLRVPAYASAVEVGTPEDRARLALHYQALGFRAMKLRLHNEEMAD
                     DLAILDACLKATQGKMRFMVDANQATSLPCPLPGVRWTYQRALRMARELEARGVLWLE
                     EPLSRYDFDNLIRLREATEIAIAGGEKNQGLHEFRWLIERGVYDIIQPDCAMSEGVSQ
                     LRKVAAMAEMYQLGFVPHHSMSGIGLAAVIHLACSFP"
BASE COUNT          570 a         1117 c         1212 g          619 t
ORIGIN      
        1 ggggaccttc ttcggcacga tcgtcggtgt tctgcccggg ttggggccga cggcatccat
       61 cgccctcctg ctgccggtca ccatcggcat ggacatggta tcggcgctga tcatgctggc
      121 ggggatctac tacggagcga tgtacggagg gtcgacgacc tccattctgg tcaacatccc
      181 tggcgaggcg gcctctgtcg tcacctgcat cgatggctac cagatggccc ggcaggggcg
      241 ggcgggcccg gcgctgggga tgtcggcctt cggctcgttc atcggcggca cggtcagcgt
      301 agtggcggtg atgctgatct cccccatcct ggccgacacc gccctgaaat tcgggccgcc
      361 cgagatgttc gccctgctgt tcttcgcgtt cacctgcatt ggcggattga cggggaagtc
      421 caaggcacag ggcctcctca tggcgagcgt cggcctcctg ctcggctcag tgggcaccga
      481 cgagatccac ggggtggatc gcttcgtctt cgggacgctc tacctccggg acgggatcgg
      541 cctggttccg ctggccatgg ggctcttcgg actcggcgag atcctgttca acatcgagca
      601 gcgagaaaca gccgggtcgg tgctggagac ccccaagaac ctcctgccga cgctgagaga
      661 ctggaaggac tcggcctggc cgatcctgcg gggaagcttt ctcggattcg gcatcggcgt
      721 tttaccggga ggcatgccgg cggtggcatc gctggtgcgc tatgccgtcg agaagcgcat
      781 tgccaggcgg ccggagctgt ttgggaaggg ggccattcag ggggtggcgg gccccgagac
      841 ggccaacaac gcggccacgg cgggggggtt cgtgccgttg ctcacgctcg ggattccgac
      901 caacagcgtc ctggcgctca tgctggcggc cctgatgctg cacggggtca cgccggggcc
      961 gctcctgctc ctcaagtggc ccgacgtgtt ctggggggtg attgccagca tgtacatcgg
     1021 caacgtgatg ctccttgtcc tcaacctgcc gatgatcggg ctgttcgtgc agcttctcaa
     1081 ggtgccctac gggatcctgt ctcccctgat catcatgttc gtcctgctgg gggcgtacgc
     1141 cctcgacaac agcgtcgccg acgtgctcct caccgtcctg ttcggcctgg caggatatct
     1201 catgaagcgg tttggactcg agcccgcccc tttggtcctc gccttcgttc tggggcccat
     1261 gctcgagaac aacctgagca aggccctcat catatcgcgg accgggagtc cgctgttctt
     1321 cttcacgcgc ccgatctcgg ccacgctgct cacactcgcg atcctggtcc tggtctaccc
     1381 gctgctgcgc tggtgtctgc gacggggtct ggggagatcg aagcgaggcg ccatcgcgta
     1441 gcgctcggcc cggatctgcc gggtcggggg agtcatggca acaccagagc gcagtcagtc
     1501 acgcactatg gacaactcac cacaggggcg aattcaatca tgaaaccgaa ggtattggtc
     1561 accgagacga tccatccagc aggctgggac aggctgcgag gcgaggtgga ggctgttgcc
     1621 tgggctgggc aagagcggca gccgctggcc ttggcagtcg gagacgtgca ggggatcctg
     1681 gtccgtgttg ccaagctcac gggtgaggtg atccaggcgg ccaagcagct caaggtcatc
     1741 gggaagcacg gcgtcggcac cgacaacatc gacatcccgg ccgccaccgc gcatggtgtg
     1801 ctcgtcacca acacaccgct ggcaaactcc acgtcagtgg cggagcacgc cctggcgcta
     1861 ctcttggggg tggcccgccg gatcggcgag tccgaccggg acttggccct cgggaggctg
     1921 cgatcgcaac ggacctacca gggcatcgag ctgtcgggaa aggtgctggg ggtgatcggg
     1981 ctggggagcg cggggctccg cctggcaggc atggcagggc aagggctggg aatgcggctg
     2041 ctcggttttg acccctacaa agcaccctgg cccgagggtg tggagcactg cggggagctt
     2101 gcgtcgctcc tccgccaggc cgacttcatc tcgatccatg tcccgctgac caaagagacg
     2161 cgaaatctga tagacgagac cgcgctggcc gcgatgaagc ctacggccat cctggtgaac
     2221 acggcgcggg gcggcatcgt ggacgaggcc gcgcttgccg ccgcgctcaa gaccggaggc
     2281 ttggccggcg cggggctgga cgtggtggtg gacgagccgc tcaagcccgg acaccccctc
     2341 ctcggcgtgc cgaacctgat cctgacgcca cacatggcag gcgtgaccga agaggccatg
     2401 atgcggatgg ccctggattc cgccgaggat atcctcaggg tgcttcgagg ggagccgccg
     2461 aagtacccgg tgaaccgaga ggtgctcacg cctgggaggc ggtagtcggc tgaacgagaa
     2521 ctggcgacga gatgccgccg ggatcggccg tcccaggccg gagtcacagg agttggtctc
     2581 atgaagatcg tggacgttct cgccaacaac ctgacgatcc cctatccggg cgaggtgcgg
     2641 cccgcctggc aaccgggtct cgtcgccacc tcgcacaact ttaccctggt tcgcatccgc
     2701 accgacgaag gcatcaccgg ttacgctggg accagcggcc atgcggccgg ggtgatctcg
     2761 gcgcaggtga agccctacct cgtcggcgag gaccccttcg ccacggagcg gttggcccgt
     2821 gtctaccgca gcgccggcaa cggcgtgtgg tgcatcgaca tggcgctctg ggacatcatc
     2881 gggaaggcgg cgaatcttcc gctgcacaag ctctggggga gctatagcct cagggtcccc
     2941 gcctatgcca gcgcggtcga ggtggggaca cccgaggatc gcgcgcggct cgccctccac
     3001 taccaggcgc tggggttccg ggcaatgaag ctccgcctcc acaacgagga gatggcggac
     3061 gatctcgcca tcctggatgc ctgtctcaag gccacgcagg ggaagatgcg cttcatggtg
     3121 gatgcgaatc aggccaccag cctgccgtgt ccgctccctg gggttcgatg gacttaccag
     3181 cgggccctcc gcatggcgcg ggagctggag gcgcgcgggg tgctctggct ggaagagccc
     3241 ctctcccgct acgacttcga caacctgatc cgcctgcggg aggcgaccga gatcgccatc
     3301 gcgggcggcg agaagaatca ggggctccac gaattccgct ggctcatcga gcggggggtg
     3361 tacgacatca tccagccgga ctgcgccatg tcggaggggg tgtcccagct ccgaaaggtg
     3421 gcggccatgg cggagatgta ccagctgggg ttcgtccccc atcacagcat gagtggcatc
     3481 gggctggcgg cggtcattca cttggcctgc tcgtttcc
//
LOCUS       DBNGZC010000057         5384 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2450533, whole genome shotgun sequence.
ACCESSION   DBNGZC010000057 DBNGZC010000000
VERSION     DBNGZC010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5384)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5384)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5384
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2450533"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..974
                     /locus_tag="AC16G0_00995"
     CDS             156..974
                     /locus_tag="AC16G0_00995"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HIR5098227.1"
                     /translation="MRLSYLFATADCKSKKVLGLRGDLDRCCAQLKAFGYDGVELMVR
                     NPRELDQVGIARTIAQHGLSVPDIGAAPLYGEDKLALTDPDPTVRQSVQGRLRDVIDF
                     SARFGAQVHVGRFRGYLPEDGGAKQALAWLTDSLLEAAEYGKSRGVRVLLEPLCHFEA
                     NTFLTVLETVEFLRSLCHPNLGVLADTFHMNIEERCLAASLMAAKGYLTHVHFADSNR
                     RYPGAGHINFTDALEALQLIGYDRFITIEVKQEPDPETAARLAAKTVRALLDTL"
     gene            1048..1176
                     /locus_tag="AC16G0_01000"
     CDS             1048..1176
                     /locus_tag="AC16G0_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098228.1"
                     /translation="MKDLRFKDAPPETTPRRVETLPAEIGMKAGAELVRRTLGYLD"
     gene            1241..2200
                     /locus_tag="AC16G0_01005"
     CDS             1241..2200
                     /locus_tag="AC16G0_01005"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5098229.1"
                     /translation="MARVLVTGGAGWLGAAVVRRLAARGDQVVVLDNFQAGAPANLAG
                     LETAVRLVPGDITDLSGLLLVIKEHRIQRIVHAAAIVSVVSSLGAPSHVTRVNIEGTL
                     NVLEAMRLFDVERTVHISSEETYGDFRCEPVDEEHPLAPTAPYGITKVASEQLGRFYR
                     IQHKTDFVNVRTSWVYGPGFPRQRFPRTLIEAGLEKRPLHLPRGGDARIDHTYLEDCV
                     DGILLALDHPSHSHDVYNISSGQSWSTSELVAMVRELIPGADLTVGPGPLWFTDEMVA
                     PKKGALDITRARKVLGYQPKYDLRQGLAANIEWYRRGRPVAHG"
     gene            2294..3253
                     /locus_tag="AC16G0_01010"
     CDS             2294..3253
                     /locus_tag="AC16G0_01010"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5098230.1"
                     /translation="MSVLVTGAMGHVGYMTALACAEAGFNVVGQYHATYHKRWAQAAG
                     PRVTWVRSDVMDFPHLLEISDKYGVEGVIHSAALPNDTLCRPVPVRSVAVNVLSTQHL
                     LELARQRKWRRVVYVSTGAVYQASDPKGFIQETDPVTPRDVYSTTKHMGELLVNMYQK
                     NYGVSACTVRVSWVWGPPLVPVGFDGPRGPIPYFLIRAVNGEKVHEPSGGEFIANFTY
                     VTDVANALLLAYRKESLSSTLYNVSNGQHYSTAEIVETIRRVLPGADVFVGPGMKPWI
                     DYTVLRGSFDLSRIRTELGFEVQYPLEKAIADYAGWLKANLVS"
     gene            3326..4285
                     /locus_tag="AC16G0_01015"
     CDS             3326..4285
                     /locus_tag="AC16G0_01015"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5098231.1"
                     /translation="MSCVLVTGGAGFLGAHLVRALALRGDRVVALDNFLTANPANLED
                     LRDRVEVVSGDITDLSHVLRVVKEHQIKRIVHTAAISSALPAIAKPALTVRVNVEGTI
                     NILEAMRIFDIQRCLHISSEETLGSFQCESADEEHPQNPFAPYAVTKMAAERLGRGYR
                     QLFGLDVISIRTSSVYGAGLPRDRPPKSFIQNALAGRPTVLPIGGDHRMDHTYIRDFV
                     DGALGAFDLPTHPYDVYNVASGRGCTIQEMAQCVRELIPGAVITVGGGLLEWIPGSTV
                     PVKGALDVTRAKETFGYQPKYDLKAGLAEYIAWFRRGQPATEI"
     gene            4303..4707
                     /locus_tag="AC16G0_01020"
     CDS             4303..4707
                     /locus_tag="AC16G0_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013653265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5098232.1"
                     /translation="MAKPTKPKAKILRAEEAFWEQPPGHFEAFSKMLVHPTNADTRHF
                     DFRISSYQPKGFAEVHVHEIAENIYYILQGRGIVELDGQRHLVEPHMVIHIPPGVRHG
                     IFNTGLEDLLFIVVASPPSDMPEVRPGRKAQP"
     gene            4804..>5384
                     /locus_tag="AC16G0_01025"
     CDS             4804..>5384
                     /locus_tag="AC16G0_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5098233.1"
                     /translation="MAEDLVLQLEGISKQFPGVKALDDVSFDLRRGEVHMLIGENGAG
                     KSTLIKILAGAHRHDTGRIVINGQPAEIQTPAQALAQGVSVIYQEFNLVPHLNVAQNI
                     FLGREPRHARFPGLIDHARMIAESQALLESLGVDIDPRATVHYLGVAQQQMVEVAKAL
                     SVNAQIVVMDEPTSALTTREIEQLFAAIRRLTAR"
BASE COUNT         1038 a         1619 c         1808 g          919 t
ORIGIN      
        1 agcaggcgag gtggctggcg aatcagggcg cagccagggg tcgagatcag gagatcccta
       61 tggcagaggt agatcacggg gcgtgcatcg actcaaaccg agagagaggt ctgtcggtac
      121 tctctggcaa agcgcccccg gaaggaatac gtgcagtgag gctgagctac cttttcgcca
      181 cagccgactg caaatcgaag aaggtcctcg gccttcgtgg ggaccttgac aggtgttgcg
      241 ctcaactcaa ggccttcggc tacgacgggg tcgagctgat ggtccggaat ccgagggagt
      301 tggaccaggt gggcatcgcc cggacaatcg cgcagcacgg cctcagtgtg cccgacatcg
      361 gtgcggcgcc cctatatgga gaagataaac tagccttgac cgacccggac ccgacggtga
      421 gacaaagcgt ccaagggcga cttcgggatg tgatcgactt ttcagcacgc ttcggggcgc
      481 aggttcatgt cgggcgtttc cgcgggtacc tgccagagga cggcggggca aagcaagcac
      541 tggcctggtt gacggacagc ctgttggagg cggctgaata cgggaagtcc aggggggttc
      601 gggtcctgct agagccgctg tgccacttcg aagcaaatac cttcctcacg gttctggaga
      661 cggttgaatt cctgcgctct ctgtgccacc ccaacctcgg cgtcctcgcc gacacctttc
      721 acatgaatat cgaggagcgg tgcctggcgg ccagcctcat ggcggccaag ggttacctga
      781 cccatgtgca tttcgcggac agtaaccgcc ggtacccggg tgcggggcat atcaacttca
      841 cggatgcgtt ggaggccctc cagctcatcg gatacgaccg gtttatcacg atcgaggtca
      901 agcaggagcc ggaccccgag acggcggcac gcctcgccgc caagacggtc cgggcgctgc
      961 tggacactct atgacgggcg gctgagatga agatcgccgg acacacgatg ggaacacccg
     1021 aatacaccct gcctggtcca tgtccacgtg aaggacctgc gcttcaagga cgcaccaccc
     1081 gagacgaccc cgaggagagt ggagaccctg ccggccgaga tcgggatgaa ggccggcgcc
     1141 gaactggttc gacggaccct ggggtatctg gactgatcga acaccgacga ggatcatcag
     1201 gccaatcgat ccacagcctg ttaccaggaa aggagcaggg atggcacgcg tgctcgtgac
     1261 agggggtgcg ggatggctcg gggcagcggt cgtgcgacgc ttggcggcac gaggcgatca
     1321 ggtggtcgtc ctggacaact tccaggcggg tgccccggca aacctggcgg ggctcgagac
     1381 cgctgtgcgc ctcgtcccgg gcgacatcac ggacctcagc gggctcctcc tcgtgatcaa
     1441 ggagcacagg atccagcgga tcgtccacgc cgcggccatc gtcagcgtgg tctcctccct
     1501 gggagcacct agccacgtca cccgggtaaa catcgagggg acgctgaacg tcctggaggc
     1561 catgcggctc ttcgatgtag aacgaaccgt tcacatctcg tccgaagaga cctacgggga
     1621 cttccggtgt gagccggttg atgaggagca tcccctggcg cccaccgccc cctacggcat
     1681 caccaaggtc gcctcggagc agttgggtcg gttctatcga attcagcaca agacggattt
     1741 cgtaaacgtc cggacctcgt gggtgtacgg cccgggcttt ccccgccagc ggttcccccg
     1801 caccctgatc gaggcagggc tggagaagcg gccgctgcat ctcccccggg gcggggacgc
     1861 gcggatcgat cacacatacc tggaggactg tgtggacggc atcctgctgg cgctggatca
     1921 tccctcgcac tcccacgacg tctacaacat cagtagcggc cagagctgga gcacctcgga
     1981 attagtggcg atggtgcgcg agctgatccc cggagccgat ctcacggtgg ggccaggtcc
     2041 gctctggttc acggacgaga tggtggcgcc gaagaaaggg gcactggaca tcacgcgagc
     2101 tcggaaggtt ctcggctacc agccgaagta cgacctgcgg caggggctgg cggccaacat
     2161 cgaatggtat cgccgcggtc ggccggtcgc gcatgggtaa cacggcgggg ggcatgttgg
     2221 acttcgtcct cctcggagtg gccagagggg cgtacaagga cgattggctc gcacggacag
     2281 gaggaagcaa aaaatgagcg tcctggtgac gggagcgatg gggcacgtgg gctacatgac
     2341 ggccctggct tgcgccgagg cggggttcaa cgtggtcggc cagtatcacg ctacctacca
     2401 caaacgctgg gcgcaggcgg ccgggccgcg ggtcacctgg gtgaggtctg atgtaatgga
     2461 cttcccacac ctgctggaga tctctgacaa gtacggggtg gagggagtga tccactcggc
     2521 cgcgcttccc aacgacaccc tttgccggcc cgtgccggtt cgctccgtgg ccgtgaacgt
     2581 tctcagcacc cagcatctgt tggagctggc gcgccagcgg aagtggcgcc gagtcgtcta
     2641 cgtcagcacc ggagcggtgt atcaggcctc ggacccgaag gggtttatcc aggaaaccga
     2701 tccggtaacg ccgagagacg tgtatagcac gaccaagcac atgggggaac tgctggtgaa
     2761 catgtaccag aagaattacg gggtcagcgc ctgcaccgtc cgggtctcct gggtgtgggg
     2821 gccgcccctc gtcccggtcg gcttcgacgg cccgcgcggc ccgatcccat acttcctgat
     2881 ccgcgcagtc aacggggaga aggtccacga gccgagcggc ggcgagttca tcgcgaactt
     2941 cacctacgtc acggacgtgg ccaatgcgct tctcctggcc tatcgcaagg agtcgctgtc
     3001 gagcaccctg tacaacgtga gcaatgggca gcattactcc acggccgaga tcgtggagac
     3061 gatccggcgc gtcctgcccg gtgccgacgt gttcgtgggc cccggaatga agccctggat
     3121 cgattacacg gtgctgcgcg ggtcgttcga tctctcccgg atccggacgg agctaggctt
     3181 cgaggtgcag tacccgctgg agaaggccat cgcggattac gccggctggt tgaaggcaaa
     3241 ccttgtttcg tgacctcaca tgtgaaggga gttgacgacc aactcctggc ggtcttcaac
     3301 ctctggaggg gggatgggag gcagcatgtc gtgcgtgttg gtgacgggcg gcgcgggatt
     3361 cctgggggcc cacctggtcc gggccttggc cctgcgcggc gaccgggtgg tggccttgga
     3421 taactttctc acagccaatc cggccaatct ggaggacctg cgggaccggg tcgaggtggt
     3481 gagcggggac atcaccgatt tgagccacgt cctccgggtg gtgaaggagc accagatcaa
     3541 gcggatcgtg cataccgccg cgatctccag cgcgctgccg gcgattgcca agccggccct
     3601 gacggtgcgg gtgaacgtcg aggggacgat caatatattg gaagccatgc gaatcttcga
     3661 tatccagcga tgcctccata tcagctcgga ggaaaccttg gggagcttcc aatgcgagtc
     3721 ggccgacgag gagcatcccc agaacccctt tgctccttac gcggtgacga aaatggcggc
     3781 cgagcgcctg ggaaggggtt atcgccaact cttcgggctg gatgtgattt ccattcggac
     3841 gtcctcggtg tacggtgcgg ggctgccccg ggatcgtccc ccaaaatcgt tcatccagaa
     3901 tgcgctggcc gggcggccca cggtgctccc gatcggtgga gaccatcgga tggaccacac
     3961 ctatatccga gactttgtgg acggggcgtt aggggccttc gaccttccga cgcaccccta
     4021 tgacgtctac aacgtggcca gcgggcgggg gtgcacgatc caggagatgg cccaatgcgt
     4081 gcgggaactg atcccgggtg cagtgatcac cgtcgggggc ggactcctgg agtggattcc
     4141 ggggagcacg gtgccagtga agggggccct ggacgtgacc cgggccaagg agaccttcgg
     4201 ctaccaaccg aaatatgacc tcaaggccgg tctggccgaa tacatcgcgt ggtttcgccg
     4261 gggacaaccg gcaacggaga tctgaggggg agggaggtat ccatggcaaa accgacgaaa
     4321 cccaaggcga agatcctgcg ggcggaggag gccttctggg agcagccgcc aggacacttc
     4381 gaggccttct cgaagatgct ggtgcatccg accaacgcgg acacacgcca cttcgatttc
     4441 cggatctcca gctatcaacc caagggcttc gccgaggttc acgtccacga gattgcggag
     4501 aacatctact acatcctgca agggaggggg atcgtggagc tggacgggca aaggcatcta
     4561 gtggagcccc acatggtcat tcacatacct cccggagtcc gacacgggat cttcaatacg
     4621 gggttggagg atctcctgtt catcgttgtg gcctcgccgc cgtccgacat gcccgaggta
     4681 cggccaggcc ggaaggcgca gccgtgagaa catgcctgtc cgagctggca aatgccgacc
     4741 ggcatttccc cgccggaaac agcatggctg aaccgggtcg gccgtcggca tggggaggga
     4801 gtcatggctg aggatttggt attgcagctc gaaggcatca gcaagcagtt tccgggggtc
     4861 aaggctctgg acgatgtcag ctttgacctg cgtcgcggtg aggtccacat gctgatcggg
     4921 gagaacgggg cgggcaaatc caccctgatc aagatcctgg caggggcgca ccggcacgac
     4981 acgggtcgaa tcgtgatcaa cgggcagccg gcggagatcc agactcccgc ccaggccctg
     5041 gcccagggcg tcagcgtcat ctaccaggag ttcaatctgg tgccgcatct caacgtggcc
     5101 cagaacatct tcctgggccg cgagcctcgg cacgcaagat ttcccggtct catcgaccac
     5161 gcccggatga tcgccgagag tcaagcgctc ctcgagtccc tcggcgtgga catcgatccg
     5221 cgagccacgg tccattacct gggggtggcc cagcagcaga tggtcgaggt ggccaaggcc
     5281 ctgtccgtga acgcccagat cgtggtcatg gacgagccga cgtccgccct gacgacccgg
     5341 gagatcgagc agctcttcgc ggccatccgg cgtctgacgg cccg
//
LOCUS       DBNGZC010000058         3276 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2548926, whole genome shotgun sequence.
ACCESSION   DBNGZC010000058 DBNGZC010000000
VERSION     DBNGZC010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3276)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2548926"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1034
                     /locus_tag="AC16G0_01030"
     CDS             <1..1034
                     /locus_tag="AC16G0_01030"
                     /inference="COORDINATES: protein motif:HMM:NF014443.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR5098234.1"
                     /translation="RAAASLQESMDLQTVGQVAGQLRTVIRVTVAAEGLGALLLYWRW
                     IGSEEVGKPAFAAVFHAVSAFCNAGFSTLTLNLVPFQTDWLTNAVIGALIVLGGLGFP
                     LFHHFASVVRSWRRVAGGPIISLHARLALMTTGVLLVGGTVACLAFEWNESLAGNSGP
                     DRLLVAGFLSVTARTAGFNTVDVGLLRPATLWALMLLMFIGGCPGSTAGGIKTTTAAA
                     MVATLLAILRGNERVQAFRRTVPAEQVSKALALVGASLVVVSAGILGLLLLEPGDPLV
                     LMFEAVSAFGTVGLSAGATARLGTIGKLLIAGLMFVGRLGPLTLAFAMAARQHAHRVR
                     YPDEKIMIG"
     gene            1040..1708
                     /locus_tag="AC16G0_01035"
     CDS             1040..1708
                     /locus_tag="AC16G0_01035"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR5098235.1"
                     /translation="MRRYMVIGLGRFGQAVAEGLTRAGAEVIGVDRNLALVDGMRDRI
                     AVAVQADSTDPEALRAAGAESVDGAIVAIGEGLEAEVLTVAICKELGVKEIIARASGE
                     REQRILELVGATRTAIVEAEMGHRLAAALAVPGVGERLPITEDISFIVREADERFFGK
                     TVAESQLRARWGLDLIGVKRPKLDGAFSVHVMPEADFRLLPKDLLLLVGANARIRSFA
                     NPEK"
     gene            complement(2418..3221)
                     /locus_tag="AC16G0_01040"
     CDS             complement(2418..3221)
                     /locus_tag="AC16G0_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5098236.1"
                     /translation="MTLGRRGRDARRLALAAAMGVVLVGCAETRSEIQTTFREGRIAQ
                     ESYYAGKAKYDHADYAGAIPHFERTLSLDPKYDEAEVYLGWSYYHQGKFPDATRYFRQ
                     VVARQPRWEGAWNGLGWSQYRAREYPVALEAFQEAVALDPTYRDAAIGLAYSLFELGR
                     YREALPHLHRLTREAEGQVFGVTAAGVEEVRSRYAWCLYYVGRYQEARAEFAKGLRAR
                     PTWAGLHSGLGWTYLKLGNRPKARESFQQALRLAPDDADARSGLAEANR"
BASE COUNT          491 a         1056 c         1158 g          571 t
ORIGIN      
        1 tgcgtgcggc tgcctccctg caggagagca tggacctgca gacggtcggc caggtggcag
       61 ggcagctccg gacagtcatc cgggtgacgg tcgcggcgga gggcctaggc gccctcctgc
      121 tctactggag gtggatcggg tccgaggagg tagggaagcc cgccttcgcc gccgtgttcc
      181 atgcagtgtc agccttctgc aatgcgggct tttccacgct cacgctgaac ctcgttccct
      241 tccagaccga ctggctgacc aatgccgtca tcggcgctct catagtcctg ggggggctgg
      301 ggtttccatt gtttcaccat ttcgccagtg tcgttcgatc ctggcggcgc gtcgccgggg
      361 ggccgatcat ctcgctccat gcccgtctcg cactgatgac caccggggtc ctcctggtgg
      421 gcggtacggt cgcctgtctg gccttcgaat ggaacgaaag cctggcaggg aactcgggac
      481 ccgaccggct cctcgtagcc ggattcctgt cggtgacggc acggacggcc ggcttcaaca
      541 ccgtggacgt tgggctcctg cgtccggcaa ctctctgggc actaatgctc ctcatgttta
      601 tcggcggctg ccccggctcg accgcggggg ggatcaagac caccacggcc gccgccatgg
      661 tcgccacact gctggcgata ctgcggggga acgagcgcgt ccaggccttc cggcggacgg
      721 tgcccgccga gcaggtctcc aaggcgttgg cattggtggg tgcctcactg gtggtggtct
      781 ccgcaggcat tctggggctc ctcctgctgg agcccggcga tcccctggtg ttgatgttcg
      841 aggcggtgtc cgccttcgga acggtgggcc tctccgcggg ggccacggcc cgcttgggga
      901 cgatagggaa actactgatc gcgggactga tgttcgtggg ccggctcggg ccgttgacgc
      961 tggcattcgc catggcggcg cgccagcacg cacatcgggt gcgctatccg gacgagaaga
     1021 tcatgatagg gtgacgggca tgcgacgcta catggtgatc gggttgggtc ggtttggcca
     1081 ggcagtggcc gaggggctga cccgggcggg ggccgaggtt atcggggtgg atcggaacct
     1141 cgccctggtg gacgggatgc gagatcgcat cgccgtggcc gtgcaagccg acagcacgga
     1201 ccccgaggcc ctgcgggccg cgggggcgga gagcgtcgac ggcgccatcg tggccatcgg
     1261 cgaagggctg gaggccgagg tcttgacggt ggccatctgc aaggagctcg gggtcaagga
     1321 gatcatcgcc cgggcctcgg gtgagcggga gcagcgcatc ctggagcttg tcggcgccac
     1381 ccgaacggcc atcgtcgagg cggagatggg tcaccggctg gccgcagccc tggcggttcc
     1441 gggcgtggga gagcggcttc cgatcaccga ggacatctcc ttcatcgtcc gggaggcaga
     1501 cgagcggttc ttcggaaaga cggtggccga atcgcagctc cgtgcgcgct ggggactgga
     1561 cctgatcggg gtcaagcggc ccaagctgga cggcgccttt tcggtccacg tgatgccgga
     1621 ggcggatttc cgccttctgc ccaaggacct gctcttgctg gtgggggcga acgcgcgaat
     1681 tcggagcttc gccaaccccg agaaatgatg cgcgcgcgtg atgccgtcct ggcgacagaa
     1741 tggcggggcc gtgggacgtc gtgtgaccca agacgtgagg gtacgaacgc tcgcctagcc
     1801 taccacacgc gcccgtctca tccgtctgtc ctccgcccgc tggtgggagg cccgttgggc
     1861 ttcatgatca gggggaggcg tttgtggggt aggccgataa gcgcgttcgt atcgcgaggc
     1921 tgctttaggg tgagcggggg ttcgatggcc tttggacatt tagggcaacc tccgagcggc
     1981 ctagcctccc ccgaagccac attttctgga cttccaggct cagcatctgc cacaggtatt
     2041 gcctcggatg gttgggtccg ggggagcgga cccataatgg agcgaactat ccgatccgtc
     2101 accgggaggc attggaaggt gatcggcatt ggttaggatg ccggggggac ggtcccctaa
     2161 tggaacagtg gatcacctct cccagcggcc gacgttggag ggtcaatttg gccgcaggcg
     2221 cttaacgcgt caagagaact catcagcaag tgaggcgcgc cgatcaggca ggcggcatcg
     2281 cggtgggacg gtcgacagca ccgactcgcc ggtcgcccgc ggctcgttgg accccaggcg
     2341 cgtcgatcgg tccctcggcg agccctattg gctcaggacc atgcgagcga ggatgtcgta
     2401 ccggataggc gacccggcta ccggttggcc tccgccagcc cactccgggc gtcggcatca
     2461 tccggcgcga gccgaagcgc ctgttggaag ctctcccgtg ccttggggcg gttcccaagc
     2521 ttgaggtacg tccagcccag cccgctgtgg aggccagccc aggtcgggcg cgcccggagc
     2581 ccctttgcaa actcggcgcg cgcctcctgg tagcgcccca catagtacag gcaccaggcg
     2641 taccgggagc ggacctcctc gacacccgcg gccgtcaccc cgaagacctg cccctccgcc
     2701 tcccgggtca ggcggtggag gtgcggcagc gcctcccgat accggcccaa ctcgaagagc
     2761 gaatacgcca ggccgatggc cgcatcgcga tacgtcggat cgagggcaac cgcctcctgg
     2821 aacgcctcca gcgccaccgg atactcccgg gcgcggtact ggctccagcc caggccgttc
     2881 caggccccct cccagcgggg ctgtcgggcc accacctggc ggaaatagcg ggtggcgtcc
     2941 ggaaacttgc cctggtggta gtagctccag ccgagataga cctcagcctc gtcgtacttc
     3001 ggatcgaggg agagcgtgcg ttcgaagtgc gggatggcgc cagcgtaatc cgcgtggtcg
     3061 tacttcgcct tccccgcgta gtagctctct tgggcgatgc gcccctcgcg gaaggtggtc
     3121 tggatctccg atcgcgtctc cgcgcagcca accaggacca ctcccatggc cgcggccagg
     3181 gcgagccgcc tcgcgtcccg gccgcgtcgt ccgagggtca tggcgtcagg tcctgcctag
     3241 gggcgcgtcg gtgtgccgcc cgacctcgtc gtcccc
//
LOCUS       DBNGZC010000059         1632 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2450723, whole genome shotgun sequence.
ACCESSION   DBNGZC010000059 DBNGZC010000000
VERSION     DBNGZC010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2450723"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="AC16G0_01045"
     CDS             complement(<1..570)
                     /locus_tag="AC16G0_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098237.1"
                     /translation="MTTPLTLLRLTPEQSSGWIWWPEYERRVVEATLLFAPRTAPEKV
                     IAHLRRLWGTAPLTLGAWMIFRDPVPGNGHRGLHTVGHLCSWVVTDEWGETSILLYQL
                     RVDRGIRATVLAQPVLDDLEDWRHEINVRYAMEQARQGQPVREVVQRVKFFTTRGMHV
                     WLRYLRGLVRVADVEEAGTLDMLTVRRERE"
     gene            complement(567..>1632)
                     /locus_tag="AC16G0_01050"
     CDS             complement(567..>1632)
                     /locus_tag="AC16G0_01050"
                     /inference="COORDINATES: protein motif:HMM:NF037897.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ADP-ribosyltransferase-containing protein"
                     /protein_id="HIR5098238.1"
                     /translation="ESPSVWDRLGELAGWSGLALPGRGGFGTAPFSAVMEGGKPKRVY
                     HGTPRVFEEFDPRKVNPEALYGPGFYFTENPEIAGGAVKEASRTVAFPTKEAAEAAPG
                     RNYFVWTDPSTGEYLTTVKETALARRGYAHAIPPEFVVDDPVQRMNIVREIDQPSAFW
                     RQRGYLSEAERAEVERRLREFEMAPKGYVPGPMGDLGLLHDMLSGQPYNIDVLKAARR
                     VGVQPNAPNVRPAYLDITKPFDMDAPVGVKELKQLGGLAMQRLGGKQKGQQNLFGPFP
                     EIGRGAELGAPPPGEEVYEELTRFLGSRAEATKILQQAGYDGITHMGGKITGSDPHRV
                     WIGFKPGQIYSSFTPPEQLP"
BASE COUNT          256 a          603 c          491 g          282 t
ORIGIN      
        1 ctcgcgctcc cgcctgaccg tcagcatgtc gagcgtgccc gcctcttcca cgtcagcgac
       61 gcgcaccaag ccccgcagat accggagcca gacgtgcatc cctcgcgtcg tgaagaactt
      121 gacgcgctgg acgacctcgc gcaccggctg gccctgtcgc gcctgctcca tggcgtagcg
      181 gacgttgatc tcgtgccgcc agtcctccag gtcgtccagc accggctgcg ccagcacggt
      241 ggcccggatg ccccggtcca cccgcaactg atagagcaga atggaggtct ctccccactc
      301 gtcagtcacc acccaggaac agaggtgccc caccgtgtgc aacccccggt gcccgttgcc
      361 cggtacgggg tcgcggaaga tcatccacgc cccgagggtc agtggagccg tgccccacag
      421 ccgccgcagg tgcgcgatca ccttctcggg agccgtgcgc ggagcgaaca gcagcgtggc
      481 ctccaccaca cggcgctcgt actccggcca ccagatccag cccgaggact gctccggggt
      541 cagacgcagc agggtcaggg gcgtcgtcac ggcagttgct ccggcggcgt aaaggacgag
      601 tagatttgcc ccggcttgaa cccgatccac acgcggtgcg ggtcgctgcc cgtgatcttc
      661 ccgcccatgt gcgtgatgcc atcgtagccc gcctgctgaa ggatctttgt ggcctcggcc
      721 cggctcccga gaaatcgcgt gagttcctca tacacttcct caccgggcgg tggcgcaccg
      781 agttccgcgc cccgcccgat ctcggggaag gggccgaaga ggttttgctg gcccttctgt
      841 ttgccgccga gtcgctgcat ggccaagccg cccagttgtt tcagttcctt gacgccaact
      901 ggggcgtcca tatcgaacgg cttggtgatg tccagatacg ccggccgcac gttcggggca
      961 ttcggctgca caccgacacg ccgggccgcc ttgagcacat caatgttgta gggttggcca
     1021 ctcagcatat cgtggagcaa gccgaggtcc cccatcgggc caggaacata ccctttcggg
     1081 gccatctcga actcccgcag acgacgctcg acctcggctc gctcggcttc ggacaagtac
     1141 ccgcgctgcc gccagaaggc gctcggctgg tcgatctcgc ggacgatatt catgcgctgg
     1201 acaggatcat ccacgacgaa ctccgggggg atcgcgtggg cgtagcctcg ccgagccagc
     1261 gcggtctctt tcaccgtcgt caggtactcc cccgtcgagg ggtcggtcca cacgaaatag
     1321 ttgcgtcccg gtgccgcttc cgccgcttcc ttggtcggga acgccaccgt gcgcgaggct
     1381 tccttgacgg cccctcccgc aatctccggg ttctccgtga agtaaaagcc cggcccgtac
     1441 agggcctcgg gattcacctt cctcgggtcg aactcctcga acactctcgg cgtcccgtgg
     1501 tacacccgct tgggcttccc gccctccatc accgcgctga acggcgctgt gccgaatccc
     1561 ccgcgtcctg gtagcgccag gcccgaccac ccggccaact cccccagccg gtcccacacg
     1621 ctcggcgact cg
//
LOCUS       DBNGZC010000060         3043 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2645885, whole genome shotgun sequence.
ACCESSION   DBNGZC010000060 DBNGZC010000000
VERSION     DBNGZC010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2645885"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="AC16G0_01055"
     CDS             <1..1053
                     /locus_tag="AC16G0_01055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5098239.1"
                     /translation="VEKDGIASACSGATFALINVSHKDPGHYAALSLESAKLFAGLEQ
                     ELGEDLDYRPVGNMSSLVEDEKELPGLEQFIRRQNQVPGLRLELLSADEARTLEPALT
                     PNILAACFSAQDGHINPYKLTVGYARAARRLGATFLTRTKVIGIEFERNRVQAVVTDQ
                     GRILTETVVVTAGIHMPEIGRMMGLTIPVVPSRGQVITTERTRPILNRPVGSLRQTED
                     GTIIIGVTYQFVGPDKQVTYDDMTRNAQRALRLFPGLRNLNVIRLWAGLRPFTVDGLP
                     ILGPVGRLKGVLLATGHSGITLAQVTGKVISELVTSGGTSTPIEEYTLERYTSARYKF
                     DMDAYARAQAQIGAGA"
     gene            1070..1366
                     /locus_tag="AC16G0_01060"
     CDS             1070..1366
                     /locus_tag="AC16G0_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR5098240.1"
                     /translation="MNGKSRRLTSNLERGRRIDLVVDGQSVSAYEGETLAAALLAAGR
                     RTFRHTVPGGHPRGVFCGIGVCFDCLVTVDGGQIRACVTPVRDGMQVSTITMVE"
     gene            1464..2849
                     /locus_tag="AC16G0_01065"
     CDS             1464..2849
                     /locus_tag="AC16G0_01065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5098241.1"
                     /translation="MREAEIVIVGGGPAGLAAALEAATAGAEVILIDEYHTLGGQFYK
                     QVPDAFSLKGGKAEGAEIIAQVAASKIEVLLGTLVWGIFEERTLALYRDEKTERIRAQ
                     KLIIAPGAQEVPVAFPGWTLPGVMMGGAAQALMVSQRILPGQRVVLAGVGPLQLKVAS
                     QFIDAGAEVVDVLEASSKPPMSMENALRSLGHWGKMREGLEYWLKLKTAGAPYHHHQV
                     PVRALGNEELEAVVVAEVDAEWRVRPGTERTLAADSLCLSYGFLPANQLPRLAGCRSE
                     YDAESGGWITRHDDNQETSVAGVYVAGEVGGIGGADVAEEEGRIAAIAALRALGKVKP
                     DGRRKEEEKIRARLAEARNFAQVTRDMMRLRPALFDLMTDETVVCRCEEVSVRAVKDA
                     IVDGDPTLRGVKIRTRAGMGQCQGRMCGSLISRLITRQTGARLEEIEPDTPRPPVKPV
                     PLSALAAGVGQ"
BASE COUNT          553 a          903 c         1112 g          475 t
ORIGIN      
        1 gtggagaagg acggcatcgc gtcggcctgt tcaggggcga cctttgccct catcaatgtc
       61 tcccacaagg atcccgggca ctatgcggcg ctgagcctgg agagcgccaa gctcttcgcg
      121 gggctcgaac aggagctggg ggaggatctg gattaccggc cggtcgggaa catgtccagc
      181 ctggtggagg acgagaagga actgccgggg ctggagcagt tcatccggcg gcagaaccag
      241 gtgcccggcc tcaggctgga gctgctgtcg gcggacgagg cgcggacact ggagccggcc
      301 ttgacgccga atattcttgc cgcctgcttc agcgcccagg acggccacat caatccgtac
      361 aaactcacgg tcggctacgc cagagccgcc cggcgactcg gggcaacctt cctcacgcgg
      421 accaaggtga tcggcatcga gttcgagcgg aaccgggtgc aggcggtcgt gaccgatcag
      481 ggccgcatcc tcaccgagac cgtggtcgtg accgcgggca tccacatgcc cgagatcggt
      541 cggatgatgg ggctgaccat tcccgtggtg ccttcgcggg ggcaggtgat caccaccgag
      601 cgaacacgtc cgatcctcaa ccggccggtg ggatccttgc gccagaccga ggatgggacc
      661 atcatcatcg gcgtcactta ccagttcgtg ggcccggaca agcaggtcac ctacgacgac
      721 atgacgcgga acgcccagcg ggccttgcga ctctttcccg ggctacgcaa tctgaacgtg
      781 attcggctct gggccggcct gcgtcccttc acggtggacg gcctcccgat tctcgggccg
      841 gtcggccgcc tgaagggtgt cctgctggcc acgggacaca gcggcatcac cctggcacag
      901 gtcaccggca aggtcatcag cgagctcgtg acaagcggcg gcacctcgac cccgatcgag
      961 gagtacacgc tggagcggta cacgtccgct cggtacaagt tcgacatgga tgcctatgcg
     1021 cgcgcccagg cccagatcgg ggcgggagcc tagcgccaag gaggaagcgt tgaacgggaa
     1081 atcgcggcga ctgacgagca acctcgagcg tggccggagg atcgacctgg tggtggatgg
     1141 ccagtcggtg tcggcctatg aaggcgagac cctggcggcc gccctgttgg cggcgggccg
     1201 ccggaccttc cgtcatactg tgcccggtgg gcacccgagg ggggtgtttt gcggcatcgg
     1261 ggtatgcttc gactgtctgg tgacggtgga cggcggtcag atccgcgcct gcgtcacccc
     1321 agtccgggac ggcatgcagg tctcaactat aacaatggtc gaatgaccaa tttccaatga
     1381 caagtgacca atgaaatccg ggctcgtcat tggtcattga gatttggtca ttggtcattg
     1441 gttcagtaag ggaggcagcg acggtgcgag aagccgagat cgtcattgtg ggtgggggcc
     1501 cggcggggtt ggccgctgcc ctggaagccg cgacggcggg cgcggaggtc atactcatcg
     1561 atgagtacca cacccttggc ggccagttct acaagcaggt gccggacgcc ttcagcctga
     1621 aaggcgggaa ggccgagggg gccgagatca tcgcccaggt tgcggccagc aagatcgaag
     1681 tcttgctggg cacgctggtc tggggcatct tcgaggagcg gaccctggcc ctctatcggg
     1741 acgaaaagac cgagcggatc cgagcccaaa agctgatcat cgcgcctggc gcccaggaag
     1801 tgccggtggc cttcccggga tggaccctgc cgggggtgat gatgggcggg gccgcccagg
     1861 ccctcatggt cagccagcgg atcctgccgg gccagcgcgt ggttctggcc ggggtgggcc
     1921 cgctgcagct caaagtggcc agccagttca tagacgcagg ggcggaggtg gtggacgtcc
     1981 tggaggcctc gagcaagccg cccatgtcca tggagaacgc cctgcgctcc ctcgggcatt
     2041 ggggcaagat gcgcgagggt ttggagtatt ggctgaagct caagacggcc ggagccccgt
     2101 accaccatca ccaggtgccg gtgcgggcgc tggggaacga ggagctcgag gcggtggttg
     2161 tagccgaggt tgacgcggag tggcgggtgc gaccggggac cgaacggacc ctggccgcgg
     2221 atagcctctg cctctcctac gggtttctcc cggccaacca actgccgcgc cttgcaggct
     2281 gccggtccga gtacgacgcg gagtcgggcg gctggatcac gcggcacgac gacaatcagg
     2341 agacgagcgt ggcgggggtg tacgtcgcgg gagaggtggg gggcatcggc ggcgcggacg
     2401 tggccgagga ggaggggcgg atcgcggcga tcgccgcgct gcgcgcgctg ggcaaggtga
     2461 aaccggatgg gcgccggaag gaagaggaga agatccgggc acgtctggcg gaggcgcgga
     2521 atttcgcgca agtgacgcga gacatgatgc ggctccggcc cgccctgttc gacctgatga
     2581 cggatgagac cgtcgtgtgt cgttgcgagg aggtcagcgt cagagccgtg aaggacgcca
     2641 tcgttgacgg ggacccgacg ctccgaggcg tgaagatccg aacccgggcc ggcatggggc
     2701 agtgtcaggg ccggatgtgc ggctcactga tctctcgctt gatcacaagg cagaccgggg
     2761 ctcgcctcga ggagatcgaa cccgacacgc ctcggccgcc cgtgaagccg gtccccttga
     2821 gcgccctggc agccggcgtg ggacagtagc atgagcctga cgggcatcgg cttcagccgc
     2881 ctgcccgtgg aggaaggggc gccatttctg aaggcaactc aatcggccga ataaccggct
     2941 caccgtgatc atccccgaca tcggcagggg aaggaggtgg agaggcgaag aggacacaat
     3001 gccaccaggg agtcgttgga tggtttcacc gaaggaggag gga
//
LOCUS       DBNGZC010000061         2595 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2352818, whole genome shotgun sequence.
ACCESSION   DBNGZC010000061 DBNGZC010000000
VERSION     DBNGZC010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2352818"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="AC16G0_01070"
     CDS             <1..1013
                     /locus_tag="AC16G0_01070"
                     /inference="COORDINATES: protein motif:HMM:NF039835.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIR5098242.1"
                     /translation="LTCTSPDPCAEVFLPGATVTLTVTAAASSDFTGWSVTPTDVTCP
                     GTDPCTVPMGVARTGTATFTLKTFALTLVKGGTGTGTVTSSPSGITCGSTCSKVFDYN
                     TVVTLTATATSPAVFTGWSGAGCSGTGTCVVTMTDLQTVTATFAPPTVALTVAVGGSS
                     GGTVTNTPLGLSCTTTCPVEVASGTPVTLTVTAGAGGAFKGWRGDCTGAGTCVVTMSA
                     PRSVTAVFSKTFTDTLTGGSTPIKAVHFTELRAAIDTLRSHYPGLGAFAWTDPTLTPG
                     STQVKRQHLLDLRTALDAAYAAAVPPKAHAAYTDPAPVTGTTPIKASHLTELRSFVQG
                     LE"
     gene            1374..1691
                     /locus_tag="AC16G0_01075"
     CDS             1374..1691
                     /locus_tag="AC16G0_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006002065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HIR5098243.1"
                     /translation="MATEKTRNATAAEATEAAPAGPKIRWDDTHMQSAYANVCNVAST
                     REEVVLLFGINQAWHSGQQEIPIQLSSRIIVSPHVAKRLSILLDNVLKEYESRFGALP
                     VSR"
     gene            1750..>2595
                     /locus_tag="AC16G0_01080"
     CDS             1750..>2595
                     /locus_tag="AC16G0_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5098244.1"
                     /translation="MDRENRTLSDEPSTPRPPRQAGAPHSHDPDQAAWRHFVEAKSPE
                     TFYLGWLALQCRFVQGVSGAVLVLGTPDTGPFAPVATWPEGGEVPSHLTQVADRALEE
                     RRGLVVPSEVAEVDGAPVRIRYAVAYPINVSGRRYGVVALDIDPRPERELQAALRQLQ
                     WGVAWVEILWLREEAGQSTAAKARLQSALDLTATAVSEDRFHSAATAFVTAMSTELGC
                     DRVSLGFLRGGKIRVRSISHTAQVTKHMNLVRSIAAAMEEAIDQKAAVAYPPGPDARP
                     QVTRAH"
BASE COUNT          442 a          917 c          838 g          398 t
ORIGIN      
        1 ggctcacctg taccagtccc gacccctgcg cggaggtctt cctgcccggg gcaacggtca
       61 ccctgaccgt cacggcggct gccagctcgg acttcaccgg ctggagcgtg acgccgactg
      121 acgtcacatg ccccggcacc gacccctgca ccgtcccgat gggggtcgct cggacgggca
      181 cggccacctt cacgctgaag accttcgccc tcaccctagt caagggcggg accggcaccg
      241 ggacggtcac ctcgagcccc agtggcatca cctgcgggag cacctgtagc aaggtcttcg
      301 actacaacac cgtggtcacg ctcaccgcca cggcgaccag ccctgccgtc ttcaccggct
      361 ggagcggggc gggctgtagc ggtaccggga cctgcgtcgt gaccatgacc gacctccaga
      421 ccgtcacggc caccttcgcc ccgcccaccg tcgccctcac ggtggcggtg gggggaagta
      481 gcggggggac tgtgaccaat acccccctcg ggctcagctg cacgacgacc tgccccgtgg
      541 aggtcgccag cggtaccccg gtcaccctga cggtgaccgc gggagcggga ggcgccttca
      601 agggctggcg gggggactgc accggggccg ggacctgcgt cgtcacgatg agtgcccctc
      661 ggtccgtgac tgcggtcttc agcaagacct tcaccgacac cctcaccggc gggagcaccc
      721 ccatcaaggc cgtgcacttc accgagctcc gggcggccat cgacaccctg cggagccact
      781 accccggcct gggggccttc gcctggacgg atcccaccct caccccaggg agcacccagg
      841 tcaagaggca gcacctgctg gatctccgca ccgccctgga tgcggcctac gcggcggcgg
      901 tcccgcccaa ggcccacgcc gcctacaccg acccggcccc cgtcaccggg accaccccga
      961 tcaaggccag tcacctgacc gaattgcgga gcttcgtgca gggcttggag taggggccta
     1021 cccccggggg gccgcacgcc ccccgatccc ccgggggagc gctatggcgt gagtctccgg
     1081 ccagaatcta cgctgatcat ccaacgacgt tccaagtgag agaagtccgg atccagggaa
     1141 ccggtccggg agtggcgtcc tcccgcgtga cgggaaccgg gtaagcctac ggagtctatc
     1201 agggatcgag gagcggttca gaccggttac gcgtccgggg ggtcgacggt ctaggcgtac
     1261 gtttttgcaa tcatacgccg attgagtctt gcatcgcaga agagttttgg gtagggttca
     1321 ggcagcatcg gcgtcgaaag taccgatcat tccacacacg aaaggagagg cagatggcca
     1381 ctgagaagac ccggaatgcc acagcagcgg aagcgaccga agccgccccc gcaggtccaa
     1441 agattcgctg ggacgacacg catatgcaga gcgcctatgc gaacgtctgc aatgtggcga
     1501 gcacgcgcga ggaggtcgtt ctcctcttcg gaatcaacca ggcgtggcac agcggccagc
     1561 aagagatccc catccagctc agcagccgga tcatcgtgag tccgcatgtg gcgaagcggc
     1621 tgtcgatcct cctggacaac gtgctgaagg agtacgagtc gcgcttcggc gcgcttccgg
     1681 tttcccggta gtcgtccccc tctctatcta taggatcccc tcggctgtcc gaggggatcc
     1741 tggataccta tggaccgcga gaaccggacc ctctctgacg agccatcgac tcctcggccg
     1801 ccacggcagg ctggcgcgcc gcattcccac gacccagacc aggctgcctg gcggcatttc
     1861 gtcgaggcga aatctcccga gaccttctac ctcggatggc ttgccctgca gtgccgattt
     1921 gtccaggggg tctccggtgc cgtgctggtc ctcggcacgc ctgatacggg cccctttgca
     1981 ccggtggcga cctggcccga aggcggcgaa gtcccgagcc acctgaccca agtcgccgac
     2041 agggcattgg aggagcgtcg cggtctcgtt gtacccagcg aggtcgcgga agtcgatggc
     2101 gcccccgtgc gcattcggta cgccgtcgcc tatcccatca atgtctccgg gcgtcgctac
     2161 ggggtcgtgg cgctggatat tgacccgcgc cccgagcggg agctccaagc agccctccgc
     2221 cagctccaat ggggtgtcgc ctgggtcgag atcctctggc tgcgcgagga ggcgggacag
     2281 agtacggcgg ccaaagcccg gctgcagagt gccctggacc tgaccgcgac cgccgtctcg
     2341 gaggaccggt tccatagcgc cgcgaccgcc ttcgtcaccg ccatgtccac cgagctgggg
     2401 tgcgatcgcg tcagcctcgg tttcctgcgg ggcggcaaga ttcgggtccg gtcaatctcc
     2461 cacaccgcgc aggtcacgaa gcacatgaac ctggtccgga gcatcgccgc ggccatggag
     2521 gaggccatcg accagaaggc ggcggtggcg taccctccgg ggccggacgc ccggccgcag
     2581 gtgactcgcg cccac
//
LOCUS       DBNGZC010000062         5438 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1374590, whole genome shotgun sequence.
ACCESSION   DBNGZC010000062 DBNGZC010000000
VERSION     DBNGZC010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1374590"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(740..2236)
                     /locus_tag="AC16G0_01085"
     CDS             complement(740..2236)
                     /locus_tag="AC16G0_01085"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017409569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0016837 - carbon-oxygen lyase activity,
                     acting on polysaccharides [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltF family protein"
                     /protein_id="HIR5098245.1"
                     /translation="MRHLVYLGTLMAAVLLIAAGRAQSQAPTKQAAAPATPSGRAGYT
                     LPNVVTLKWVGDLDGMIKRRQIRILTAYSKTYYFVNRAVQRGVAYDIGQLLETDLNKK
                     LRGKHLRVRVLMVPVARDEIIPALLEGRGDVAMANLTITPRRMEQVDFTNPFIQNVAE
                     ILVTGPQAEPIGSIQELSGKEVYLRKSSSFHESIQTLNAEFARTGLAPVKVRPAPETL
                     EIEDILEMVNAGLVKATIVDRHMAEFWRQIFPKLTLHPDIAVRTGGEVGWMVRKNSPQ
                     LKAELNAMLARYPEGSTRRNMILQKYLKDTKWAKAATSEEELAKFERTVAFFRKYGFR
                     YNMDYLLMVAQGYQESQLNQAARSPAGAIGVMQLMPTTGKEMRVGDIRQLEANVHAGV
                     KYMRFMMDQFYADEPMDWLDKGLFTFASYNAGPGRIQQLRGLAAKRGLDPNKWFNNVE
                     LVAAEKIGRETVTYVSNIYKYYLAYEMLTEQRAEREKAKETIWRGESM"
     gene            complement(2526..3131)
                     /locus_tag="AC16G0_01090"
     CDS             complement(2526..3131)
                     /locus_tag="AC16G0_01090"
                     /inference="COORDINATES: protein motif:HMM:NF015823.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HIR5098246.1"
                     /translation="MGSLLQCISAAGCVIALLGVSGCGGTKETRFYVLTPLPAAERPG
                     DASAGENPAIGLLPVSLPEQLDRPEIVTRMGENMLQLAEFDRWAAPLQENFTRVLAGN
                     LAVLVPTERLSVFPWGKAGSPDYEVAVEVARFEGTLGGDCSLVARWSVLKRDGKRVAV
                     TGTSSYREPAGDSYATLVAAHSRLVAALSRDIAAALKAVPQ"
     gene            complement(3140..4240)
                     /locus_tag="AC16G0_01095"
     CDS             complement(3140..4240)
                     /locus_tag="AC16G0_01095"
                     /inference="COORDINATES: protein motif:HMM:NF014523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIR5098247.1"
                     /translation="MGRRPNPAIIGGFVVGAVALAVAGLVVFGGGKFFRHTQRWVAYF
                     DESIKGLAIGAPVTFRGVKIGSVTDIKAVIDRQARQIRTPVFFQLEADRITDPEGRQV
                     AFLPEEAGSRLLIERGLRAQLEVQSFVTGQLAINLDFHPDASLHLVGASPGYPEFPTV
                     PSAIAAIRKRVEEIDLGELSGDVKDMVQGINRMVNSPEVKQVLVSAVESLEGVKKLAA
                     TADAKVASLGPALERSTVSINDTLETIQQLVRRVDSQTVPAVNETLRDAQQVVRHVDA
                     ETVPAMNQVLADLRPLVAEVGRTAGAARAALEQAQKALATAETAVEEGSPLQYQMRLT
                     LQEVAGAARAVRALSSYLERHPDAVLFGKDGK"
     gene            complement(4244..5008)
                     /locus_tag="AC16G0_01100"
     CDS             complement(4244..5008)
                     /locus_tag="AC16G0_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5098248.1"
                     /translation="MAPAKACIQVRNLDMAYGDRVIQHDLDFTVGQGDIFIIMGGSGC
                     GKSTLLRHLIGLIRPARGDVFYDGQNFWGAAPDERDQMLRRIGVLYQSGALWSSMTLA
                     ENVALPLGEYTALSPAEIRDLVSFKLALVGLAGFEDFYPSEISGGMRKRAGLARAMAL
                     DPDILFFDEPSAGLDPISSRLLDNLILELRESLGATVVVVTHELASIFAIGTNSVFLD
                     AETKTMLATGSPKRLLAEPPHPTVRRFLTRGEAEGG"
     gene            complement(5021..>5438)
                     /locus_tag="AC16G0_01105"
     CDS             complement(5021..>5438)
                     /locus_tag="AC16G0_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MlaE family ABC transporter permease"
                     /protein_id="HIR5098249.1"
                     /translation="QVNEEIDALTTLGISPMEFLVLPRMLALAVMMPLLCIYADLMGI
                     LGGGSVGLGMLDLGSVQYYLRTASAIRLTDVAAGLIKSGVFGVLVAIAGCLRGMECGR
                     SSAAVGWAATSAVVTGIVCIIVSDALMTVIFDLIGF"
BASE COUNT          970 a         1821 c         1655 g          992 t
ORIGIN      
        1 gatggccgga acgatggcaa gtgcatggag cgcgagggta acgaggatga ttagctcagc
       61 cgcagacgcc gcacgcatgc ccccgctgct cctccccgaa ctgggcacca gcgtttcgag
      121 tcgcgtatac tgtgacttta cattgccagc ctagcctctt caagaccgca gttaactctg
      181 atccggaatt cggcatctcg ccaagatcac ccaactccag gctgggtacc cggttcgcca
      241 agacccacga gcatattctg ggcacagcag gcaagagacc caccgcccct ggaaggggtc
      301 atccgatatg ggggattccg tgattaaacc tggcctttct ttgcgccctt ggcgtctttg
      361 cgagagacgg atttcttcgg attggggtta acagatactc cgccattgcg agaacaccag
      421 ggatatactt gagagatgcg aggaaatgtc aaggagaaga gccatcctgc caggggccac
      481 aagggtttct ctcgcccccg gtttgaaacg gactcttgtt caattggcag tccgggggac
      541 cggaccccaa atggagcaat ggatctcctc ttccgctagc aaacggcggg gaaccgcttt
      601 agctgggggg gcagttagct cgataaaaac agtggacttc cgcaggctct tcaggcttca
      661 agtcgaccaa tccaggcact ggcgggagtg agttccgatc ccacacgcga tacttccaaa
      721 ccttcgcgat ggcgtcgcgc tacatgcttt caccccgcca aatcgtttcc ttggccttct
      781 cacgctccgc gcgttgctcg gtgagcatct cgtacgccag gtagtatttg tagatgttcg
      841 agacgtacgt caccgtctcc cggccgatct tctcggcggc aacaagctcg acattgttga
      901 accacttgtt gggatcgagg ccgcgtttgg cggcgagccc gcggagctgc tggatgcggc
      961 ccgggccggc gttgtaggag gcgaaggtga agagcccctt gtcgagccaa tccatcggct
     1021 cgtccgcgta gaactgatcc atcatgaagc gcatgtactt cactccggcg tggacgttgg
     1081 cctccagctg ccggatgtcg cccacccgca tttccttgcc ggtggtgggc atcagctgca
     1141 tcaccccgat cgcccctgcc ggacttctgg ccgcctggtt gagctgggat tcctggtagc
     1201 cctgcgccac catcagcagg tagtccatgt tgtagcggaa accgtacttc cggaagaacg
     1261 cgaccgtccg ctcgaacttg gcgagctcct cttccgacgt cgcggccttc gcccacttgg
     1321 tgtccttcag gtacttttgg agaatcatgt tgcgtcgggt cgagccctcg ggatagcggg
     1381 cgagcatagc gttcagctct gccttgagct gcggactgtt cttccggacc atccatccga
     1441 cctcgccgcc ggtgcggacc gcgatgtccg ggtgaagggt gagcttgggg aagatctgcc
     1501 gccagaattc ggccatgtgt cggtccacga tggtcgcctt gaccaggccg gcgttcacca
     1561 tctcgaggat gtcctcgatc tcgagcgtct ccggtgccgg ccggaccttc accggcgcga
     1621 ggccggtccg ggcgaactcc gcgttgagcg tttggatact ctcatggaag ctcgacgact
     1681 tgcgaagata gacttccttg ccggagagct cctggatgct gccgatcggc tcggcctgcg
     1741 gcccggtgac caggatctcg gcgacgttct ggatgaaggg attggtgaag tccacctgct
     1801 ccatgcgtct cggggtgatc gtcaggttgg ccatggcgac atccccccgc ccctccagca
     1861 gggccgggat gatctcgtcg cgggccacgg ggaccatcag cacccgcacg cggaggtgtt
     1921 tgcccctgag tttcttgtta agatccgtct ccaggagttg cccgatgtcg taggccacgc
     1981 cgcgctggac cgcccgattc acaaagtaat aggtcttcga gtatgcggtc agaatccgaa
     2041 tctgccgccg cttgatcatg ccgtcgagat cgccgaccca cttcagcgtg acaacattgg
     2101 gcagcgtgta gcctgcccgc cctgatggag tagccggcgc ggcggcctgc ttggtcgggg
     2161 cctgggattg tgcgcggccc gcagcgatca agagaacggc agccatgagc gtaccaagat
     2221 acaccaagtg tctcatggtg ttcctcctcg cagcgtgctc tggtcggcct gcactccgtg
     2281 cgggaaaacc caacccgccc aagacaatga gaccaggaag tatgaccagc ctcttcagca
     2341 ttcaataaat cgtcgtggtt agccgaatcc acagtctcct aatcagccgc cgcgagcctt
     2401 cgaaatatag ccacagctgc caagtcttca ggaaagtcca ccggacatgg gtcaccgctt
     2461 atgagcccta ttcagtggcc tgccagaagg agcaaacgga gacggcctgg tcccacgacg
     2521 cagccctact gcggaacagc cttgagcgcc gcggcgatgt cccggctgag cgcggccacc
     2581 aggcggctat gggccgccac cagcgtggcg taactgtcgc cggccggctc gcggtaactc
     2641 gacgtccccg tgacggccac tctcttccca tctctcttga gcacggacca gcgcgcgacg
     2701 agcgagcaat cgccccccaa ggttccctcg aaccgggcca cctccacggc cacctcgtag
     2761 tcgggggaac cggccttccc ccaggggaac acgctcagac gttccgtggg gacgaggacc
     2821 gccaggttgc ccgccagcac ccgcgtgaag ttctcttgga ggggagccgc ccaccggtcg
     2881 aactccgcaa gctggagcat gttctcgccc atccgggtca cgatctccgg ccgatccagt
     2941 tgctcgggga gggaaaccgg aaggaggccg atggcgggat tctccccggc agaggcatcc
     3001 cccggccgct cggcagcggg caggggagtc aggacgtaga acctcgtttc cttggtcccc
     3061 ccgcagccgc tcacacccag gagggcgatc acgcaccctg cggccgagat gcactgcagc
     3121 agagagccca tcagtcgcct cactttccgt ccttgccgaa cagcacagca tcgggatgtc
     3181 gctccagata gctggacagg gcgcgcaccg cgcgggccgc tcccgccacc tcctgaagcg
     3241 tgagccgcat ctgatactgc aggggcgagc cctcctccac cgccgtctcc gcggtggcca
     3301 gggccttctg ggcctgctcc agggcggccc gggccgcccc ggcggtcctt ccgacctccg
     3361 ccaccagcgg ccggaggtcc gccaggacct ggttcatggc cggcacggtc tcggcgtcga
     3421 catgccggac gacctgctgg gcgtctcgca gggtctcgtt cacggccggg acggtctggc
     3481 tatccacgcg gcgcacgagc tgctggatcg tctccaaggt gtcattgatg gacacggttg
     3541 atctttcgag agccggtccc aggctggcca ccttggcatc cgcggtcgct gccagcttct
     3601 tcaccccctc cagcgactcg acggcactga ccagcacctg cttgacctcc ggcgaattga
     3661 ccatacgatt gatcccctgc accatgtcct tcacgtctcc gctcagttca ccaaggtcta
     3721 tctcctccac gcgtttcctg atggcggcga tggctgaggg gaccgtgggg aattccggat
     3781 acccgggcga tgccccgacc aagtgcagcg aagcgtcagg gtggaagtcg aggttgatgg
     3841 ccagttggcc ggtcacgaag ctctgcactt ccagttgtgc gcgcagaccg cgctcgatca
     3901 aaagccttga gcctgcctct tccggcagga acgccacttg cctcccctca ggatcggtga
     3961 tgcggtccgc ttcgagctgg aagaagacgg gggtacgaat ttgcctcgcc tgccggtcga
     4021 tgaccgcctt gatgtcggtg acactcccga tcttgacccc ccgaaacgtg accggcgcgc
     4081 cgatggcgag acccttgatg gattcgtcga agtacgccac ccagcgctgg gtgtgccgga
     4141 agaatttccc cccgccgaag accaccaggc cggcgactgc cagggccacc gcgcccacca
     4201 cgaatccccc gatgatcgcc ggattgggcc tacgtcccat gactcatccc ccttccgcct
     4261 ccccgcgcgt gagaaatcgt cggacggtcg ggtgcggcgg ctctgccagc aggcgcttcg
     4321 ggctcccggt ggccagcatc gtcttggtct ccgcgtccag gaacacggag ttggtgccga
     4381 tggcgaagat gctggccagc tcgtgcgtca ccaccaccac cgtggcgccg aggctctccc
     4441 gcagctccag aatcaggttg tcgagcaggc gggagctgat cggatccagg ccggccgacg
     4501 gctcgtcgaa gaacaggatg tcggggtcca gggccatcgc ccgcgccagc ccggcccgct
     4561 tccgcatgcc cccgctgatc tccgacggat agaagtcctc gaagcctgcc aggccgacta
     4621 gggccagctt gaacgaaacc agatcccgga tctccgcagg actgagggca gtgtactcgc
     4681 ccagcggcag cgccacgttc tccgccaggg tcatggagct ccagagggcc ccgctctgat
     4741 agagcacgcc gatccggcgg agcatctggt cgcgctcgtc cggtgcggcg ccccagaagt
     4801 tttgcccgtc gtagaacacg tcgccccggg cgggccggat cagcccgatc aggtgtcgca
     4861 ggagcgtact cttgccgcac ccgctccctc ccatgatgat gaagatgtct ccctgcccca
     4921 ccgtgaaatc caggtcatgc tggatcaccc ggtcgccgta tgccatgtcg aggttgcgca
     4981 cctggatgca cgccttggct ggtgccattc cctcactacc tcagaagcca atgagatcga
     5041 agatcaccgt catcagggcg tcggacacga tgatgcagac gatacccgtc accaccgccg
     5101 acgtggcagc ccagccgacg gccgcggagc tgcggccgca ctccatcccg cggagacacc
     5161 cggcaatcgc caccaggacg ccgaacaccc cgctcttgat caggccggcc gccacgtcgg
     5221 ttagtcgaat ggcggaggcc gtccggaggt agtactgcac gctgcccaga tccagcatgc
     5281 ccaggccgac gctgccgccg cccaggatgc ccatgaggtc tgcgtagatg cagaggagcg
     5341 gcatcatcac cgccagggcc agcatacggg ggagcaccag gaattccatg ggggagatcc
     5401 cgagggtcgt cagggcgtcg atctcctcgt tcacctgc
//
LOCUS       DBNGZC010000063         3219 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1569929, whole genome shotgun sequence.
ACCESSION   DBNGZC010000063 DBNGZC010000000
VERSION     DBNGZC010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1569929"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..781
                     /locus_tag="AC16G0_01110"
     CDS             92..781
                     /locus_tag="AC16G0_01110"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR5098250.1"
                     /translation="MNARTIAVRREQVPRLPLRIAHLSDIHIGSIGSSALAAIIAKTN
                     DLRPDVVLITGDLFDNANSTTRLAAAQLRAFVAPVLFTSGNHEAYAGYGNVGQMLSAT
                     QVRWLRNERVDFKGVRIIGVDNSYGTELLQSVLARTSPAPAFTLLMNHQPTGFAVAAR
                     HQVGLTLSGHVHNGQIWPFNYVVGFFYPYLKGLHENAGAFLNVSTGTGIWGPPMRLGS
                     RSEIVLLEPAP"
     gene            886..1632
                     /locus_tag="AC16G0_01115"
     CDS             886..1632
                     /locus_tag="AC16G0_01115"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR5098251.1"
                     /translation="MRHNRAKEKLAAGKPISVVAPSYTSAGLVELLGRMGFDAIFIDC
                     EHGPAGWDEVENMVRAAELTDATPIIRVQSNDPSTITRALDRGAGGVQVPHINTREEA
                     QAAVRAAKFAPLGQRGYAGGRSALGVKGNFTLHANEETMLVAMLEEAEALKNLDTILR
                     VDHIDAFFIAPGDLSQSMGYPGQVDHPDVQAAIHDAVRRTRAAGRAPGVLTTNAESAR
                     RYLALGALFIYVSLASLLQPGAAEFLSVFR"
     gene            1664..3157
                     /locus_tag="AC16G0_01120"
     CDS             1664..3157
                     /locus_tag="AC16G0_01120"
                     /inference="COORDINATES: protein motif:HMM:NF014763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR5098252.1"
                     /translation="MTWPSVSSLRVRLLLLVLLALIPARGLGIYTAWQMRQDARAESL
                     QDAMRLARIASTAGERLVEGIHQILIVLAHLPEVRRQNVSACSALFADLLKPYPYYSN
                     LGAAKPNGDVFCSAVPYEGVANVGDRTYFQRAIQRRDFAPGEYQIGRITGKAVINIGY
                     PVLDGGGQVAAVVFAGLDLSWLNRLLAESRLPENSTITVVDGAGQVLARYPEPEKWVG
                     KLFPDAPFIRAAISQQEEGTVEGSGLDGTPRLFAFTRLQVWERERFAYVYIGIPRDSA
                     FARVNQIDTRSLVGFGGVLVFALLATGIGSRMLILHPVTALVQTIQRLGAGDLSARTG
                     LPHTPSEMGRLAAALDEMAGGLEARQAGASSMTGLLQECEERFRRLADSAPAMLWLSG
                     PDGLRTFFNRAWLDFTGRSPAEAAGSGWLASVHPEDRDPYLHASIAALAARREFRVEY
                     RLRRADGEYRRILDAGIPRILPDGSVVGYVGTGTDVIGGEQAATTSR"
BASE COUNT          542 a         1048 c         1055 g          574 t
ORIGIN      
        1 gcagatactg acgcggttta accccctccc gccaatggtg tgggccatcg gggtttgctg
       61 gcttgcctga ggtctcgccc tgtacgctgt catgaatgcc cgcacgattg ccgtccgccg
      121 ggaacaagtc ccccgtctgc cactcaggat cgcccacctg tccgacatcc acatcggctc
      181 gataggatcc tcggcgctcg ccgccatcat cgccaaaacc aatgacctca ggcccgacgt
      241 cgttctcatc accggggatc tgttcgacaa cgccaactcc accacccgtt tggctgccgc
      301 ccaattacgg gcattcgtcg ccccggtctt gttcacctcg ggcaatcacg aggcctacgc
      361 tggatatggc aacgtgggcc agatgctttc ggccacacag gtccgctggc tgcgcaatga
      421 aagggtggac ttcaaggggg tccgaatcat cggcgtggac aacagttacg gcacggaact
      481 gctgcagagc gtgctggccc gcacgtctcc tgcgccggcg ttcacgctcc tcatgaacca
      541 ccagccgaca ggctttgccg ttgccgcccg tcaccaggtc ggcctgacgc tctccggcca
      601 tgtgcacaac ggccagatct ggccgttcaa ctacgtcgtg gggttctttt atccctatct
      661 caaaggtctg cacgagaacg caggcgcatt tctgaatgtc tcgaccggca ccggcatctg
      721 gggcccgccg atgcgcctcg gctcacgcag cgagatcgtg ttgcttgaac cggcgccatg
      781 aaagccgcgt cgaccttgag catttctgga tagcctatcg gagggagcag agacgtcatt
      841 tcgcggggaa acggggatct gcccttcaaa gataaggagc ggagcatgcg acacaatcgc
      901 gccaaggaaa agctggctgc cgggaaaccg atcagcgtgg tggccccgag ctacacctcg
      961 gccggactgg tggagttgct cggtcggatg ggattcgacg cgatcttcat cgactgcgag
     1021 cacggtccgg ccggctggga cgaggtcgag aacatggtgc gcgcggccga acttaccgat
     1081 gccaccccaa tcatccgggt gcagtcgaat gacccgtcga ccatcacccg ggcgctcgac
     1141 cggggggcgg gcggcgtgca ggtgccgcac atcaacacgc gggaggaggc ccaggcggcg
     1201 gtccgggcgg ccaagttcgc gccgctgggt cagcgtggct atgccggcgg ccgctccgcc
     1261 ctcggcgtca aagggaattt caccctgcac gccaatgagg agaccatgct ggtggcgatg
     1321 ctcgaggaag cggaggcgct gaagaatctg gacaccatcc tgagggttga ccacatcgac
     1381 gccttcttca tcgctccggg tgatctctcg cagtcgatgg ggtacccggg tcaggtggac
     1441 catccggacg tgcaagcggc gattcacgac gccgttcgtc gcacgcgggc ggctggtcgc
     1501 gcccccggcg tcctgaccac gaatgcggag agcgcccgcc gctatctcgc cctgggagcg
     1561 ctgttcatct acgtctcgct ggcgagcctg cttcaacccg gcgcggctga gtttctctcg
     1621 gtcttcaggt gatgcaccga agcggagagg ggaggtcgga tccatgacgt ggccctccgt
     1681 gtcgagcctt cgggtgcgcc tgctgctcct tgtcctcctg gccctcatcc ccgcccgcgg
     1741 ccttgggatc tacacggcct ggcagatgcg ccaagacgcc cgggcggaat cgctccaaga
     1801 cgccatgcgg ctggctcgga tcgcctccac cgcaggggag agactggtcg aggggataca
     1861 ccaaatcctc atcgtgctgg cgcacctccc cgaggtccgc cggcagaacg tctccgcctg
     1921 tagcgcactc ttcgcggacc tcttgaagcc gtacccctac tattcaaacc tcggcgcggc
     1981 caaaccgaat ggcgacgttt tctgcagcgc ggtcccgtac gagggcgttg ccaatgttgg
     2041 ggaccggacc tattttcagc gagcaatcca gcggcgtgac tttgctcccg gagagtatca
     2101 gatcggccgc atcaccggaa aggcggtcat caatatcggc tacccggtcc tggatggcgg
     2161 tgggcaggtg gcagccgttg tcttcgccgg gctagacctg tcgtggctca accgcctcct
     2221 cgccgagtcg cgattgcccg agaattcgac gataacggtg gtggatgggg ccggccaggt
     2281 cctggcgcgc tacccggagc cagagaagtg ggtcggcaag ctcttcccgg acgcgccgtt
     2341 catccgggct gccatctccc aacaggagga aggcactgtg gagggctcgg gcctggacgg
     2401 cacgccccgc cttttcgcgt tcacccggct ccaggtctgg gagcgggaac ggttcgcgta
     2461 tgtgtacatc ggcattccac gggacagcgc ctttgccagg gtgaaccaga tcgacacccg
     2521 gagcctcgtc gggtttggag gggtcctggt gtttgcgctg ctggccacag ggatcggatc
     2581 gaggatgctc atcctgcatc ccgtgactgc gctggtgcag accatacagc ggctcggcgc
     2641 cggggacctc tccgcgcgca ccgggttgcc ccacaccccc tcggaaatgg gccggctcgc
     2701 cgcggccctg gatgagatgg ccgggggttt ggaggcgcgg caggccgggg cgtcctccat
     2761 gacagggctt ctccaggaat gcgaggagcg ctttcgcagg ctggccgaca gtgcgccggc
     2821 catgctgtgg ctatcgggac cggatggcct tcggaccttc ttcaaccgcg cctggttgga
     2881 cttcacgggc cggagtccag cggaggctgc ggggagtggc tggctcgcca gcgtgcatcc
     2941 cgaggatcga gacccgtacc tgcatgccag tatcgccgca ctggcggccc gccgggaatt
     3001 ccgcgttgaa taccggctac ggcgcgcaga tggcgagtac cgtcgcatcc tggacgcggg
     3061 cattccacgc attctcccgg acgggagtgt ggtgggctat gtgggaactg gaacggatgt
     3121 catcgggggt gagcaggccg cgacgacatc gcgctagcct ccacagtgga tgggccgagc
     3181 ggccgggtac gaccgcgcac cagcggccga atccacgag
//
LOCUS       DBNGZC010000064         3071 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_592060, whole genome shotgun sequence.
ACCESSION   DBNGZC010000064 DBNGZC010000000
VERSION     DBNGZC010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_592060"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..351)
                     /gene="rpmI"
                     /locus_tag="AC16G0_01125"
     CDS             complement(154..351)
                     /gene="rpmI"
                     /locus_tag="AC16G0_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015750507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR5098253.1"
                     /translation="MPKMKSNRGASKRFKTTGTGKLRRAHSSKSHLLTGKTTKRMRRL
                     RHGALVDKTDLKRMRVLFPYG"
     gene            complement(484..993)
                     /gene="infC"
                     /locus_tag="AC16G0_01130"
     CDS             complement(484..993)
                     /gene="infC"
                     /locus_tag="AC16G0_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR5098254.1"
                     /translation="MSKGLRVNERIRIKEVRVIGPDGAQLGILPIEQALQTAFKLQMD
                     LVEVAPEARPPVCRIMDYGKYRYEQAKKAREAKKKQTIIELKEIKLRPKTEEHDFQFK
                     AKHAERFLKEGNKAKITMMFRGREMVRMDRGKALLDRFVEVLKEVGVVEQAAKVEGRN
                     MTMILAPKH"
     gene            complement(1030..3042)
                     /gene="thrS"
                     /locus_tag="AC16G0_01135"
     CDS             complement(1030..3042)
                     /gene="thrS"
                     /locus_tag="AC16G0_01135"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR5098255.1"
                     /translation="MAGAELILMDGRRAQIPAGAPILASLPGLGEPGTKGAVVVRWNG
                     QLLDFMTPAPADAKLELVPADSPEGLQVIRHSAAHLMAAAVQVLFPDAKFAIGPAIED
                     GFYYDMDLPRPLVPEDLPIIEAKMTELAGRRLSYVRREIRMEEALTWAHDHRQDYKVE
                     ILEAIRQHGTSRPETHEPRAASGAEELGELASEGTPGTDMVSFYQTGDFVDLCRGPHV
                     PDTTWLKAFRLTHIAGAYWRGDERRPMLQRIYGTAFLTKEALERHLFQLEEARRRDHR
                     RLGQDLELFSVADDVGGGLVLWHPKGGIVRKVVEDFWRDEHLKNGYDLVYTPHVGRAK
                     LWQTSGHLGFYSDLMYPRMEREGQEYYLKPMNCPFHIMIYQSRVRSYRDLPLRFAELG
                     TVYRYERAGVLHGLLRVRGFTQDDAHIFCTPEQMDEEITRAVKFSLFFLRTFGFKDYV
                     VYVATRPDKFVGDLATWDAATEALKRASERAGQTYEVDPGGGSFYGPKIDIKVKDALG
                     RPWQCTTVQFDFNMSERFGLSFIGEDNRQHRPFMVHRALLGSMERFLGVLIEHYGGAF
                     PTWLAPVQARVLPVADRHHAYAESLVTRLKTEGLRGEADLRNEKIGFKIREAEVQKIP
                     FMLIVGDKETAAGTVSVRLRGGQDKGAMPLAELVRVLAAENSPQVA"
BASE COUNT          521 a         1051 c          890 g          609 t
ORIGIN      
        1 gatcttggct ttattgcctt ccttcaagaa tcgctcggcg tgttttgaac ccaccctttg
       61 ctcgtggcat gacctctatc cctcgcttcc ccggtgtctc cgaatcctga accgccgatg
      121 agaatccgca gcctgcaatc agacgaatcc ggtttacccg tacgggaaca gcaccctcat
      181 ccgcttgagg tctgtcttgt ctaccagcgc cccatggcgg agcctccgca tgcgtttggt
      241 cgtcttcccg gtcagtagat ggctcttcga cgagtgggcg cgccggagct ttccggtgcc
      301 cgtggtcttg aaccgctttg acgctcctcg gttgctcttc atcttgggca tgaacgagat
      361 ctccttgcga ctacgggaca gacccacccg tcgggtgcaa aaattcttca ggaccacccg
      421 ccagccatcg atgacgtcga ccccttctga tcgtccgtca tccctgcact gcaccagcgg
      481 ccgctagtgc ttgggcgcca ggatcatcgt catgttccgc ccctcgacct tggcggcctg
      541 ctccaccaca cccacctctt tcaggacctc cacgaaccgg tcaagcaggg ccttcccccg
      601 gtccatgcgg accatctcgc ggccgcggaa catcatggtg atcttggctt tattgccttc
      661 cttcaagaat cgctcggcgt gttttgcctt gaactggaaa tcgtgctcct cggtcttcgg
      721 gcgaagcttg atttccttca attcgatgat ggtctgtttc tttttcgcct cccgggcctt
      781 cttcgcctgc tcgtaccggt acttgccgta atccatgatc cggcagaccg ggggtcgagc
      841 ctcgggggca acttcgacca ggtccatctg cagcttgaac gccgtctgga gcgcttgctc
      901 gatcggcagg ataccaagct gggccccgtc gggaccgatc acacgtactt ccttaatgcg
      961 gatccgctcg ttgacgcgaa ggcctttact tatggaccct acctcccgcc gcggttggcg
     1021 gccgccggat caggcgacct gcggcgaatt ttcagcagcc agtacgcgga cgagctccgc
     1081 caggggcatc gcgcctttgt cctggccgcc ccgcaggcga accgacaccg tcccggctgc
     1141 cgtttccttg tctcccacaa tgagcatgaa cggaatcttc tgaacctccg cctcgcgaat
     1201 cttgaagccg attttttcat tccgcaggtc tgcctcaccc cgcaggccct ccgtcttgag
     1261 ccgcgtcacc aagctctcgg cgtaagcgtg gtgccgatcc gccaccggca agacccgcgc
     1321 ctgcaccggg gccagccagg tcgggaacgc gcccccgtag tgctcgatca ggacccccag
     1381 aaatcgctcc atggagccga gcagtgcgcg gtgcaccatg aagggtcggt gttgacggtt
     1441 atcctcgccg atgaagctga gcccgaaccg ctcggacatg ttgaagtcga actgcacggt
     1501 cgtgcactgc caggggcggc ccagggcatc cttcaccttg atgtcgatct tgggcccgta
     1561 gaacgacccg ccgccggggt ccacctcata ggtctgaccg gcacgctccg acgcccgctt
     1621 cagggcctcg gtggctgcgt cccaggtcgc cagatctccc acgaacttgt cgggccgcgt
     1681 ggccacgtaa acgacgtagt ccttgaaccc aaaggtccgg aggaaaaaca ggctgaactt
     1741 gaccgcccgc gtgatctcct cgtccatctg ctccggtgta cagaagatgt gggcatcgtc
     1801 ctgggtgaac ccgcggaccc ggagcaaccc gtgcagcacg ccggcccgct cgtagcggta
     1861 caccgtcccc aattcggcga accggagcgg caggtcccgg taactgcgca cacgggactg
     1921 gtagatcatg atgtggaacg ggcagttcat gggtttgagg taatactcct gcccctcacg
     1981 ctccatccgc gggtacatga gatccgagta gaatccaaga tgaccgctcg tctgccacag
     2041 cttggcccgc cccacgtgcg gtgtgtagac gaggtcatac ccgttcttca ggtgctcatc
     2101 ccgccagaag tcctcgacga ccttccggac gatgccgccc ttggggtgcc acaggaccag
     2161 gccaccgccg acgtcgtcgg ccacgctgaa cagctccagg tcctgcccca gccgccggtg
     2221 atcccgtcgg cgggcttcct ccaactggaa gagatgccgt tccagggctt ccttcgtgag
     2281 gaaggccgtt ccgtagatcc gctggagcat cgggcgacgc tcgtccccgc gccagtaggc
     2341 acccgcgatg tgcgtgaggc gaaaggcctt cagccaggtc gtgtccggaa cgtgggggcc
     2401 gcggcacagg tccacaaagt ccccggtctg gtagaagctg accatgtctg tgcccggagt
     2461 gccctcactt gccagctcgc ccagctcctc cgctccgctc gcggctcgcg gctcgtgggt
     2521 ctcgggtctc gaagtcccgt gctgcctgat ggcctccaag atctcgacct tgtagtcctg
     2581 ccggtgatcg tgcgcccagg tcagcgcttc ttccatccga atctcgcggc ggacatagga
     2641 aagccgccgc ccggccaact ccgtcatctt cgcctcgatg atcggcaggt cctcgggcac
     2701 gagcggcctg ggcaagtcca tatcgtagta aaagccgtcc tcgatggccg gcccgatggc
     2761 gaactttgca tcgggaaaga gcacttggac ggcggccgcc atgaggtgcg cggcgctgtg
     2821 acggatcacc tgcaagccct cgggggagtc cgccggcacc agctcgagtt tggcgtcggc
     2881 gggcgcaggc gtcatgaagt ccaggagctg cccgttccat cggacgacca cggccccctt
     2941 tgtcccgggc tcacccagcc ctggcaagct ggcgagaata ggcgcaccag cggggatttg
     3001 ggcgcggcga ccatccatca ggatcagttc ggcaccagcc atgggtcacc acagaacaaa
     3061 aaagcaccac g
//
LOCUS       DBNGZC010000065         3578 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1668500, whole genome shotgun sequence.
ACCESSION   DBNGZC010000065 DBNGZC010000000
VERSION     DBNGZC010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1668500"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="AC16G0_01140"
     CDS             <1..917
                     /locus_tag="AC16G0_01140"
                     /inference="COORDINATES: protein motif:HMM:NF023746.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051258 - protein polymerization [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR5098256.1"
                     /translation="EDTDKILQHLQGADMVFITAGLGGGTGGGAAPIIANLAKEMEIL
                     TVGVVTKPFLFEGKRRGEQAERSLEELKANVDSLIIIPNQRLINHASKNTGVKEAFAM
                     ADNVLRQAVQGIADIIVKPGYINVDFAVVRTVMSERGLAMMGIGEAADDNAAVDAVQK
                     AINSPLLENVSIKGARGVLLNFTHASTMPIYQLNEASKIVHDMVDKEANIIFGDVLDD
                     DMAGDVRVTVIATGFDEANSEPVEEVPRVTELRAYTPKPAPAPATAPDRGTYLRKGKK
                     VVGGAQLPLEDLTEEVLDIPTILRRQAD"
     gene            complement(967..1899)
                     /locus_tag="AC16G0_01145"
     CDS             complement(967..1899)
                     /locus_tag="AC16G0_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007504704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098257.1"
                     /translation="MDKDRDSQDQGGVSQPTLDALAVASGSARESRSLWSDARSRLLR
                     NPVAMAGGVVSILLILVAFAAPYIAPYDPVHQDLANSLAGPSMMHLAGTDVHGRDIFS
                     RIIYGTRISLRIGFLGMLLGCVVGVILGLVAGFYGGWWDTVIMRLMDVQLAFPGLLVA
                     ICIIAIIGPGLENVIVAVGIFSVPLFARVTRGQVLTLKEQEFIVAARMLGAQDGRIML
                     THLLPNAVAPLLVLCTLRIATAILTAASLSFLGLGAQPPTPEWGAMLSDGRAYLAIAP
                     HVATTPGLAILITVLSFNLLGDGLRDALDPRLKV"
     gene            complement(1892..2812)
                     /locus_tag="AC16G0_01150"
     CDS             complement(1892..2812)
                     /locus_tag="AC16G0_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011228608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098258.1"
                     /translation="MLRYILRRLAYMTPILFGVSLVIFLILHLVPGDPARLVAGLDAT
                     QEDVKIIRSSLGLDRPLPVQYLSWLGNVSHGDLGRSTRTHRPVTQELRLTFPATVELT
                     LSATVVAVLIGVAAGTTAAERSNSIFDYSSMVVALLGVCTPSFWLGLMLMLVFAVKLD
                     LLPTTGRGGLENLVLPAVTLGAGAAAIIARVTRSSMLEVLGMDYIRTAHAKGLAARAV
                     VLRHAVKNALIPVITVIGLEFGYLLAGAVVTETVFAYPGVGWLLVEAIGFRDYPVIQG
                     ALLLLALQFALVNLVVDVLYAVVDPRISYG"
     gene            complement(2910..>3578)
                     /locus_tag="AC16G0_01155"
     CDS             complement(2910..>3578)
                     /locus_tag="AC16G0_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098259.1"
                     /translation="LNYAVDKQAVIKAVLRGQGKPADSPLAPRVWGYTPVKTYPYDPA
                     KAKALLTEAGYPNGFKAVLRGNNQTDTKDAMVAIQGQLKQVGVVVEVLALPAAALSAE
                     RFLPQAENKGEMDFAGWSPSTGDADWGIRPLLTKDNWPPTNFTIGFYTNPKLEAAIKA
                     GLETADAAKRKAAYAEAQRVIMDDCPWIFLWVNNLLGGSRANVGGISLQPDAIGFYRS
                     VYYK"
BASE COUNT          679 a         1134 c         1233 g          532 t
ORIGIN      
        1 tcgaagatac cgacaagatc ctccaacacc tgcagggggc cgacatggtt ttcatcactg
       61 ccggcctggg cgggggcacc ggcgggggcg cggcccccat catcgccaac ctggcgaaag
      121 agatggagat cctgaccgtc ggggtggtca cgaagccgtt cctcttcgag ggaaagcgcc
      181 ggggcgagca ggccgagcgg agcctggaag agctcaaggc caacgtggac agcctgatca
      241 tcatcccgaa ccagcggctc atcaaccatg ccagcaagaa caccggggtg aaggaggcct
      301 tcgccatggc ggataacgtc cttcgccagg cggtgcaggg catcgccgac atcatcgtga
      361 agcccggcta catcaacgtg gacttcgccg tcgtccggac cgtcatgtcg gagcgcggtc
      421 tggccatgat ggggatcggc gaggcggccg acgacaacgc cgcagtggac gcggtgcaga
      481 aggccatcaa cagcccgctc ctggaaaacg tctccatcaa gggggcgcgc ggggtgctct
      541 tgaacttcac ccatgcctcc accatgccca tctaccagtt gaacgaggcc tccaagatcg
      601 tccacgacat ggtggacaag gaggccaaca tcatcttcgg ggacgtcctg gacgatgaca
      661 tggcggggga cgtgcgcgtg acggtcatcg ccacgggctt cgacgaggcg aacagcgagc
      721 cggtggagga ggtgccgcgg gtcacggagc tgcgggccta tacgcccaag ccggcgccgg
      781 ctccggcgac cgcaccggac cgggggacct acctgcggaa ggggaagaag gtcgtgggag
      841 gggcgcagct ccccctggag gatctcaccg aggaggtcct ggatatcccg acgatcttac
      901 gacgacaggc cgattgagcc ggccgcggcg tgggtgaagc tgtccacgga cacggcggga
      961 cgactctcac accttcagtc gtggatccag ggcatcgcgc aggccgtcgc ccaggaggtt
     1021 gaaggagagg accgtgatca gaatggccag accgggggtc gtggccacgt gcggagcgat
     1081 agccaggtat gctcggccgt cgctcagcat ggcgccccac tcgggggtag gcggctgcgc
     1141 cccgagcccc aggaagctga ggctggccgc cgtgaggatg gcggtggcga tgcgtagcgt
     1201 gcagaggacc aggaggggtg ccacggcatt gggcagcagg tgggtcagca tgatgcggcc
     1261 gtcctgcgct cccagcatcc gggccgccac gatgaattct tgctccttca gggtgagcac
     1321 ctgcccgcgg gtcacacggg cgaacagggg caccgagaag atgcccacgg ccacgatgac
     1381 gttctccagc cccggcccga tgatggcgat gatacagatg gccaccagga gccctgggaa
     1441 agccagctgc acgtccatca ggcgcatgat gaccgtgtcc caccagccgc cgtagaagcc
     1501 ggcgaccagg ccgagtatca cccccaccac gcaccccagc agcatcccca gaaagccgat
     1561 ccgcagggag atccgggtcc cgtagatgat gcggctgaag atgtctcgcc cgtggacgtc
     1621 agtgccggcc aggtgcatca tcgagggtcc ggccagggag ttggccagat cctggtggac
     1681 cggatcgtag ggggcgatgt aaggggcggc aaacgccaca aggatcagca agatcgagac
     1741 gacgccaccg gccatggcca ccgggttccg cagcagacgc gaacgggcgt cgctccacag
     1801 acttcgcgac tcacgcgcgg agcccgacgc caccgcgagc gcatccaagg tcggctgact
     1861 cacacccccc tggtcttgcg agtctcggtc tttatccata cgagatccgc gggtctacaa
     1921 cggcgtagag tacatccacg accagattca ccagggcaaa ctgcaaggcc agcaagagca
     1981 gcgcaccctg gatcaccggg tagtcgcgga agccgatcgc ctcgaccagc agccacccca
     2041 ccccaggata ggcaaagacg gtctccgtca ccacggcccc ggccagcagg tagccgaact
     2101 ccagcccgat gacggtgatc acggggatca gggcgttctt caccgcatgg cggagcacca
     2161 ccgcccgggc ggcgaggccc ttggcgtggg ccgtgcggat gtagtccatg cccagcactt
     2221 ccagcatgga ggagcgtgtc acccgggcga tgatcgccgc cgccccggcg cccaaggtca
     2281 cggccggcag caccagattc tccaggccac cgcgaccggt ggtggggagg agatcgagtt
     2341 tcaccgcaaa cacgagcatg agcatgaggc ccagccagaa ggaaggggtg cacaccccca
     2401 atagggccac caccatgctg ctgtaatcga agatggagtt actccgctcg gccgcggtcg
     2461 tccccgcggc cacgccgatg aggacggcga cgacagtggc gcttagggtc agctccaccg
     2521 tggccggaaa ggtgaggcgc agctcctgcg tcaccggccg atgcgtcctc gtcgagcgtc
     2581 ccagatcgcc gtgggacaca ttgcccagcc aggagaggta ctggaccggc agggggcgat
     2641 ccaggcccag gctggagcgg atgatcttca catcctcctg cgtcgcgtcg agcccggcca
     2701 ccaggcgcgc cggatcgccg gggaccaggt ggaggatgag gaagatcaca agggagaccc
     2761 cgaagaggat gggggtcatg tacgcaaggc ggcggaggat gtagcgtagc atggctcacg
     2821 aggagacagg cagtgggaga cgggagccgg gtgtctggac ctcgtctccc ccgacctgtt
     2881 tctggtttcc tttcccggag gtgcggccgt tacttgtagt acacggatcg gtagaatccg
     2941 atcgcatcag gctggagtga gatcccgccc acgttggcgc ggctgccgcc cagcaggttg
     3001 ttcacccaga ggaagatcca ggggcaatcg tccatgatga cccgctgggc ttcggcgtat
     3061 gcggccttgc gcttggccgc gtcggccgtc tcgaggcccg ccttgatcgc cgcctcgagc
     3121 ttggggttgg tgtagaagcc gatggtgaag ttggtgggtg gccagttgtc cttggtcagc
     3181 agggggcgaa tgccccagtc ggcatcgccg gtggaggggg accagccggc aaagtccatt
     3241 tcccccttgt tctccgcctg gggcaagaat cgctccgcgc tgagggcggc agccggcaag
     3301 gcgaggacct cgacgaccac gcccacctgc ttgagctggc cctggatggc caccatggca
     3361 tccttggtgt ctgtctggtt gttcccgcgc aggacggcct tgaagccgtt gggatagccc
     3421 gcctcggtca ggagggcctt ggccttggcc ggatcatagg ggtaggtctt caccggggtg
     3481 tagccccaga cgcggggcgc caggggagaa tctgccggct tcccttggcc ccgcaggacc
     3541 gccttgatga cggcctgctt gtcgacggcg tagttcaa
//
LOCUS       DBNGZC010000066         3848 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_885727, whole genome shotgun sequence.
ACCESSION   DBNGZC010000066 DBNGZC010000000
VERSION     DBNGZC010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_885727"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="AC16G0_01160"
     CDS             complement(<1..330)
                     /locus_tag="AC16G0_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098260.1"
                     /translation="MQGCRPDWVKESGRDPDSVYLEEDALQTARDLTQALYTAAVALG
                     RTRDAEEVVRVAIAETQRAAGVEAVALHLLDASREVLLLRELAGTTAAFREQMGVLPL
                     SEAPLAEI"
     gene            complement(508..1851)
                     /locus_tag="AC16G0_01165"
     CDS             complement(508..1851)
                     /locus_tag="AC16G0_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR5098261.1"
                     /translation="MSVAYPSQRVAYPYFVVALLLFVLQVLVGTWLAVNYWASLPQWV
                     VDVFPFSTARAIHTNLLVLWLLLGFMGSTYYLVPDETGRELYSPALAYLQLVILAGTG
                     VVGIVGFLFYWTEGKPLLELPRPLDFLIVIGALMFLFNVGMTMLKARNWTAIQGSLLG
                     GLVFLALLYLFDLPFYRNLTIDWYYWWWVIHLWVEGAWELVTAAMTAWILMKITGVPR
                     ASVEKWLYVELGLFLFTGIAGTGHHYYWLGAPRYWLWVGGIFSALEPVPIVLMLWDAW
                     QHVKERQIPIRNRVVFTYAIGMAIFHLVGAGVWGFIHTLPQINYYTHGSQVTASHGHM
                     AFFGAYVLVNLMFFYYAMPELRGVAGFPDRRGHIAFWWMCLCMVGMGLSFGVAGVLQA
                     YIERVMGLGFMTAQAQMRFWFAVTWVFGLCLLVGVVLAVVDLLSLRAAPAAQERA"
     gene            complement(1879..2562)
                     /locus_tag="AC16G0_01170"
     CDS             complement(1879..2562)
                     /locus_tag="AC16G0_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5098262.1"
                     /translation="MSARAAKWIFYVGTLISAALFLGLTLDTHRQIQSLTHADQLSEQ
                     VVAGKRVWQKYNCNDCHTILGFGGYYAPDMTKAYWRRGDEGIKAVVRTPEKFTTWRKM
                     PRLPVSEQELTQLVAFLKWTSEIDTHDWPPQDAKLRRTAQAAVGIGVSPGATLFKEKG
                     CFGCHRLYETGGALGPELTHVGGRLDRNTLEKIMEDPKSVNPKAIMPAPVLSNDERDQ
                     MADFLTGLK"
     gene            complement(2729..3652)
                     /locus_tag="AC16G0_01175"
     CDS             complement(2729..3652)
                     /locus_tag="AC16G0_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR5098263.1"
                     /translation="MAVGLYILVFSLKLAVYFATGVVVILAEALHSLSDVLISSFLLA
                     ASIWSRKEADEVHMFGYGRAQNVAALVAATLFISLTSFKVFEEAIPRLFRAGESSYQN
                     LALALGVIIVSMVLAALPLIGLFREKNRGAAAKAQLLELVNDELGLLAALIATLLVAW
                     GLPIADPIAAIAVATIIAVNAVGLLRENLSVLLGRSPGPEVLGKVERLARSVEGVLGV
                     HELRAEYIGPDTVRAGFHIDVERGLPIEQADRVAEEVRQRVHQATGCRYCVIHVDPVK
                     PERTPPTVKDAPTPGAVRPPAEGSDASPPPS"
BASE COUNT          769 a         1281 c         1157 g          641 t
ORIGIN      
        1 aatctcggcg aggggtgcct cggaaagtgg caggacgccc atctgctccc ggaatgcggc
       61 ggtggttccg gcgagttctc taagcaagag cacctcccgg ctcgcatcca ggaggtgcag
      121 cgccacggct tccacgccgg cggcgcgctg cgtctcggca atcgccacgc ggaccacctc
      181 ttctgcgtcc cgagtacgcc cgagcgccac cgccgcggtg tagagcgcct gcgtcagatc
      241 cctggccgtc tgcaaagcat cctcttccag atacaccgaa tcggggtccc gccctgactc
      301 tttcacccag tccgggcggc acccctgcat tcaaggccaa attggctgaa ctgtcaagtc
      361 atccgggaag ggaaagatgc gcggcagaga caggacggat tcctgctagg gcctgacgcc
      421 ttgatccctc atcgtcccaa ggaatacagc cggagccacg aggctccggc tgtgtgttcc
      481 attccgcctg aggagattct tccccgatca ggctctctcc tgggcggctg gggccgcacg
      541 caggctgagc agatccacca ccgccaggac cacccccacc agcaggcaga ggccgaagac
      601 ccaggtcacg gcgaaccaga agcgcatctg ggcctgggcg gtcatgaagc cgaggcccat
      661 gacccgctcg atgtacgcct ggagcacccc cgccacgcca aagctgaggc ccatgcctac
      721 catgcacagg cacatccacc agaaggcgat gtgaccccgc cgatccggga agccggcgac
      781 acctcgcagc tccggcatcg catagtaaaa gaacatcaga ttgaccaaaa cgtatgcccc
      841 gaagaaggcc atgtgcccgt gggaggccgt cacctgactg ccgtgggtgt aatagttgat
      901 ctgcggcagg gtgtggatga agccccagac gcccgccccg acgaggtgga agatcgccat
      961 cccgatggcg taggtgaaga ccacccgatt ccggatcgga atctggcgtt ccttcacgtg
     1021 ctgccatgca tcccagagca tcaggacgat cggcaccggc tccagggcgc tgaagatccc
     1081 gccgacccag agccagtagc gcggcgcacc gagccagtag taatgatggc ccgtgccggc
     1141 aatccccgtg aagaggaaca gccccagctc gacgtagagc catttctcga cggaggcccg
     1201 gggcaccccg gtgatcttca tcaggatcca ggcggtcatg gctgcggtca ccagctccca
     1261 ggccccttcc acccagaggt ggatgaccca ccaccagtag taccagtcga tcgtcagatt
     1321 ccggtagaac ggcagatcga agaggtagag cagcgccaaa aagaccagac cgcccagaag
     1381 cgatccctgg atggccgtcc agttccgggc cttgagcatg gtcatcccga cgttgaacag
     1441 aaacatcagc gccccgatga cgatcaggaa gtccaggggg cggggcagct ccagcagcgg
     1501 cttcccttcg gtccagtaga agaggaagcc tacgatcccc accacgccgg tcccggccag
     1561 gatgacgagc tggagatagg ccagggccgg gctgtacagc tctcgtccgg tctcatcggg
     1621 gacgaggtag taggtgctgc ccatgaagcc caggaggagc cacaggacca ggagatttgt
     1681 gtggatcgcg cgcgcggtgc tgaagggaaa gacatccacc acccactggg gtagggacgc
     1741 ccagtagttc acggccagcc aggtccccac gaggacctgc agaacgaaca gcaggagcgc
     1801 gaccacgaag tacgggtacg ccacgcgctg agatgggtag gcaacggaca tgagtagatc
     1861 cctcctctcc gccccagatt atttcaatcc cgtgaggaaa tcagccattt gatcccgctc
     1921 gtcattggag agcaccggcg cgggcatgat cgccttgggg ttgacagatt tcggatcctc
     1981 catgatcttc tcgagggtgt tccggtccag ccgcccgccc acgtgggtga gttccggccc
     2041 cagcgccccg cccgtctcat acagccggtg gcacccgaag caccccttct ccttgaagag
     2101 cgtggccccg gggcttaccc cgatgcccac cgctgcctgg gcggtccgcc ggagcttggc
     2161 atcctggggc ggccagtcgt gcgtgtcgat ctcgctggtc catttcagga aggcgacgag
     2221 ctgggtaagc tcctgctcgc tgacgggaag ccggggcatc ttgcgccagg tggtaaactt
     2281 ctctggcgtg cggacgacgg ccttgatgcc ctcatcgcca cggcgccagt aggccttggt
     2341 catgtccggt gcgtagtaac cgccgaaacc gaggatggtg tggcagtcgt tgcagttgta
     2401 cttctgccag acccttttcc ccgccacaac ctgctcgctg agctgatcgg cgtgggtcag
     2461 gctctggatc tgcctgtgcg tgtccagcgt gagccccagg aagagtgcag cggaaatcaa
     2521 tgtgcctacg tagaagatcc actttgcagc cctcgcactc ataggcgatc cctccttcct
     2581 gcatgatgac tgcgagaaag acaccgatca atccgagtcg gagcaaatca ggctctacaa
     2641 ttagtagaca ccggtcatcc ccgggtatga ccaggatcat attctcggcc tgcgccgctg
     2701 ccgcgggcat cagcccgccg atcagcactc aggatggcgg aggagatgcg tcgctcccct
     2761 cggcaggcgg tcgaaccgca ccgggtgtag gggcatcctt gacagtcggg ggagttctct
     2821 ccggtttgac cggatccacg tggatcacac agtatcggca tcccgtggcc tgatgcactc
     2881 gctggcgtac ctcctcggcg acccgatccg cctgctcgat gggaaggccc cgctccacat
     2941 cgatgtgaaa tcccgcacgg accgtatccg gcccgatata ctcagcccgc agctcatgga
     3001 cacccagcac tccctcgacg gagcgcgcca gacgctccac cttccccagg acttcgggcc
     3061 ccggcgagcg tcccagcagg acggagagat tctcacgcag caggccgacg gcgttgaccg
     3121 caatgatcgt ggccacagcg atggcggcga ttggatccgc aatcggcagg ccccaggcga
     3181 cgagcagcgt agcgatcagg gcggccagca gacccagctc gtcattcacc agttccagga
     3241 gctgggcctt ggctgcagcc ccgcgatttt tctctcggaa caggccgatc agcggaaggg
     3301 cggcaagcac catcgagacg atgatcacgc ccagcgccag cgccagattc tggtaggagg
     3361 attctcccgc tcgaaagagc cggggaatcg cctcttcgaa aaccttgaag ctggtgagcg
     3421 agatgaacag ggtcgccgcc acgagggcgg ccacattctg cgcccgaccg taaccgaaca
     3481 tgtggacttc atccgcctct tttcgggacc agatcgaggc cgccagcaag aagctggata
     3541 tgaggacgtc gctcaggctg tgcaatgcct ccgccaggat caccaccacc ccggtcgcaa
     3601 aatagaccgc aagcttcagg ctgaagacca ggatgtacag gcctaccgcc atcttcagtg
     3661 agcgagtctc cccccctgct tcagcgacct tcacttctgc ccccaccctg gcgaggctac
     3721 gccgtcggac acagacgggc tcaattcttc ttctgcgtcc ggcgcagctc ttccgccatg
     3781 gtcttgatct ccgtcagatc gcgcgccatc tcgctccacc cgtaccagag cgaatagtcc
     3841 gggctggc
//
LOCUS       DBNGZC010000067         3736 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1473020, whole genome shotgun sequence.
ACCESSION   DBNGZC010000067 DBNGZC010000000
VERSION     DBNGZC010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1473020"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="AC16G0_01180"
     CDS             complement(<1..515)
                     /locus_tag="AC16G0_01180"
                     /inference="COORDINATES: protein motif:HMM:NF016974.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HIR5098264.1"
                     /translation="MSHIEPVAYIETWQLATSLFVRQDLFNAVTLRQETPGSPHAATR
                     SIFLMGPKFDDPPDVSLWFQDVPQFKYPILAEWPVAEVMLEEVIRVVNGHTWGKAMIV
                     ELAPGSAVAWHVDEGAYAEAHDRYHLPLVTNPFAYLYSGGEQIHASPGVLVKFDTRVL
                     HSAANFGQSPR"
     gene            complement(512..1684)
                     /locus_tag="AC16G0_01185"
     CDS             complement(512..1684)
                     /locus_tag="AC16G0_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098265.1"
                     /translation="MPTQSLTVTAPAGATSVVVTGAFGTGYQAVVTPAWNATVVVSAK
                     TPSQFTVTFGTPAPPGGSTFDAVVETAGAPVSPSITCVQAGTLMTRMRAKIPDSVFIN
                     DVPQPEMDGKFRAQTIYGWFDDAVQVLTQLTGWTMTDWWAMPQVEFQPFYDVDVRWVS
                     MESAFSNQWPVDVIQMEESDTIWPQTSGQLSQQSLTAYVRRLGTNLQVGLWPTPNLDD
                     PQANLVGAISDSSTAPITLTTTANFLSFGYVQIDNEILAYQLVTTPSPTGIAVISRGI
                     GGTTPAAHADNAVVQHLGFWIKGRRLPGPIVNSLSCVEVPRGWISHLETYVLGQCRME
                     QKRFAEGDKLLANFEKACRNIDADPNWKVNRGLIPAYGSPRVGPIYFPGGGGVVVP"
     gene            complement(1712..2230)
                     /locus_tag="AC16G0_01190"
     CDS             complement(1712..2230)
                     /locus_tag="AC16G0_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098266.1"
                     /translation="MRRYTALALAAFKDQRGAFAMTVQGFGSVIQTPVMQVAGPVPPI
                     QIADAAFPASAGTGDRLVGATQNLVYACSAFRVVIYLKAFVLGTSSAAGNGPLFSVEG
                     SNSATFASGIYASGLHQAQNIAGATLTQEIEILWRAPVVPVQYFRIVVDSTLSTGGSM
                     AGTYDALIEAAP"
     gene            complement(2310..3284)
                     /locus_tag="AC16G0_01195"
     CDS             complement(2310..3284)
                     /locus_tag="AC16G0_01195"
                     /inference="COORDINATES: protein motif:HMM:NF033394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HIR5098267.1"
                     /translation="MAGVIGSGLFQPPVTLINTADAITKKYFTPLLADSIFKPSPVWW
                     SFTRLGKKVRGGGSIVWPSIMSEETTGGAYWGVQKLDNTPTDPIQPAEIQWKGYQEAI
                     VIPVMDALLNEGEGQVIDLVKSKDETAMGSMLQKLSRAMQGVAPQNTAIDLDNIPEAL
                     GALGGTYAGITLASPWACNGGSGPSAGGPVSFANMMSDYMSASQGNEQPDRCFTTANG
                     YTNFWGLLTQQQRQIEDSETIRAGFKMHFMFNNAVVMWDAFVPAGEMQWFTSKYARAV
                     FHSRDYFSVDPFIQPSDQRVLIARIYVLMNLQFFTLRQHARRTGITNA"
     gene            complement(3339..>3736)
                     /locus_tag="AC16G0_01200"
     CDS             complement(3339..>3736)
                     /locus_tag="AC16G0_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098268.1"
                     /translation="GKQYVLKREYEGILRNLGDRYTTRYNPDGKGSSWDQQQFLKFVM
                     DNQVPNLEVAYRAYTADQEAEFRARQAEEAGVERGRKAAEVPRIPFGRRRPAPKPKDL
                     PETFQEVSVDQIAEDPDIQAAMAKDYGAE"
BASE COUNT          661 a         1227 c         1163 g          685 t
ORIGIN      
        1 atacgcggcg actgcccgaa gttcgccgcc gagtgcagca cacgggtatc gaacttcacg
       61 agcacgccgg gggaggcatg gatctgctct ccgcccgagt agagataggc aaagggattg
      121 gtcacgagcg gcagatggta gcgatcatgc gcctcggcat aggccccctc atccacgtgc
      181 cacgccacgg cacttccggg agccagttcc actatcatcg ccttgcccca ggtatgacca
      241 ttgaccacgc gaatgacttc ttccagcatg acctccgcca ccggccactc ggcgaggatc
      301 gggtacttga actgcggcac gtcctgaaac cacagactca cgtcgggcgg atcatcgaac
      361 ttcgggccca tgaggaagat cgaccgagtg gccgcgtgag gactcccggg cgtctcctgc
      421 cgcaaggtca ccgcattgaa gaggtcttgc cgcacaaaga gggacgtggc gagttgccac
      481 gtctcgatgt acgccaccgg ctcgatgtgg ctcatggcac cactacaccc ccgccacccg
      541 ggaagtagat gggccctacc cttggactcc catacgccgg aatcagacca cggttgacct
      601 tccagttcgg gtccgcgtcg atgttgcgac acgccttctc gaagttggcg aggagcttgt
      661 cgccctcggc aaaccgcttt tgctccatgc ggcactgacc gagcacgtag gtctcaaggt
      721 ggctgatcca cccacggggc acctcgacgc aggacaggct gttgacgatg gggccgggaa
      781 gcctccggcc cttgatccag aagccgagat gctgcaccac agcgttgtcg gcatgggccg
      841 cgggcgtggt gcccccgatc cctcggctga tgacggcaat ccccgtgggc gagggcgtcg
      901 tcacgagttg ataggccagg atctcgttgt cgatctgcac gtacccgaac gacaggaagt
      961 tagccgtcgt cgtgagggta atgggcgccg tagacgagtc gctgatcgcc ccaacgagat
     1021 tagcctgcgg gtcgtccagg ttcggggtcg gccagaggcc cacctggaga ttcgtgccca
     1081 ggcgtcggac ataggccgtc agggattgct gactcaactg cccgctcgtc tggggccaga
     1141 tggtgtccga ctcttccatc tggatcacat cgacgggcca ctgattgctg aaggccgatt
     1201 ccatgctcac ccagcgcacg tccacgtcgt agaacggctg gaactccacc tgcggcatcg
     1261 cccaccagtc cgtcatggtc cacccggtca gttgggtgag tacctggacc gcatcatcga
     1321 accagccata gatggtctgc gcccgaaact tcccgtccat ctcgggctgg ggcacgtcgt
     1381 tgatgaagac cgaatccggg atcttggccc gcatccgggt catgagcgtc cccgcctgga
     1441 cacacgtgat cgagggggac acgggcgccc ccgccgtctc caccaccgca tcgaaggtcg
     1501 agcctccggg gggagccgga gtcccgaagg tcaccgtgaa ctgggagggg gtcttggcgg
     1561 aaacgacaac ggtggcgttc caggccggag tgaccacggc ctggtagccc gtgccaaagg
     1621 cccccgtcac cacgaccgag gtggcccctg cgggcgcggt gacggtgagg ctttgagtcg
     1681 gcacgagcgc ctccttgagc gcgagcgagt gttacggagc cgcttcgatc aacgcatcgt
     1741 aggtgcccgc catgctccca cccgtcgaga gcgtggagtc caccacgatg cggaagtact
     1801 ggacgggcac gaccggcgcc cgccacagaa tctcgatctc ctgcgtgagc gtggcccccg
     1861 cgatgttctg cgcctgatgc agccccgagg catagatccc cgaggcgaac gtggcgctgt
     1921 tggagccctc gacactgaac agcgggccgt tccccgccgc cgagctcgtg ccgagcacga
     1981 acgccttcag gtagatcacc acccggaacg ccgagcaggc atacacgagg ttctgcgtgg
     2041 cgcccaccag ccgatctccg gtgcccgccg aggcggggaa cgccgcatcc gcgatctgga
     2101 tgggcggaac cggccccgcc acctgcatca ccggagtctg gatcaccgac ccgaatccct
     2161 gaaccgtcat tgcgaaggcc ccgcgctgat ccttgaatgc ggcgagcgcc agagccgtgt
     2221 accgcctcat gctgtctctc atgtcaaccc tctttccgct ccctatggag ccgagcaatg
     2281 tagcgggcag aaccgccaac cattagtgat tacgcattgg tgatcccggt cctgcgggca
     2341 tgctgacgca gggtgaagaa ctggagattc atgagcacgt agatgcgggc aatcagcacg
     2401 cgctgatcgc tcggctggat gaacggatcg acgctgaagt agtcccggga atggaagacc
     2461 gcccgggcgt acttgctggt gaaccactgc atctcgcccg cgggaacgaa ggcgtcccac
     2521 atgaccacgg cgttgttgaa catgaagtgc atcttgaagc cggcgcggat ggtctccgag
     2581 tcctcgatct ggcgctgctg ctgcgtcagg aggccccaga agttcgtgta gccgttcgcc
     2641 gtggtgaagc agcggtcggg ctgttcgttg ccctgcgagg cactcatgta atccgacatc
     2701 atgttcgcga aactgaccgg gccgccagcg ctcggccccg acccaccgtt acacgcccac
     2761 ggggacgcga gggtgatccc ggcgtaggtg ccgccgaggg ctcccagcgc ctccgggatg
     2821 ttgtcgaggt cgatcgccgt gttctgcggg gccacgccct gcatggcccg gctcaacttc
     2881 tggagcatcg agcccatcgc cgtctcgtcc ttcgacttga ccagatcaat gacctggccc
     2941 tcgccctcgt tgagcaaggc atccatgacg ggaatgacga tggcttcctg ataccccttc
     3001 cactggatct ccgcgggctg gatggggtcg gtgggcgtgt tatcgagttt ctggacgccc
     3061 cagtaggcgc cgcccgtcgt ctcctcactc atgatcgagg gccagacgat cgagccgccg
     3121 cctcggacct tcttccccag tcgggtgaac gaccaccaga cggggctggg cttgaagatc
     3181 gagtcagcca gcagcggcgt gaaatacttc ttggtgatcg cgtccgcggt gttgatgagt
     3241 gtgaccggag gctgaaacag acctgaaccg attacaccgg ccataatacg cgctcctttc
     3301 tctgtgtggc cgggtcaggc ccgttgccct gcccggactt actctgcgcc gtagtccttc
     3361 gccatcgccg cctggatatc cgggtcttcg gcgatctgat cgacactgac ttcctggaac
     3421 gtctccggca gatccttcgg cttgggcgcg gggcgccgac gaccgaacgg aatccggggc
     3481 acctccgcgg ccttccggcc tctctccacc cccgcctctt ccgcctgtct cgcccggaac
     3541 tcggcttcct gatcggcggt atacgcgcgg taggcgactt cgagattggg gacttggttg
     3601 tccatcacga acttgaggaa ttgctgctga tcccacgacg atcccttgcc gtctgggttg
     3661 tagcgcgtgg tgtagcgatc cccgaggttt ctcagaatcc cctcgtactc tcgcttcagg
     3721 acgtactgct tgccgt
//
LOCUS       DBNGZC010000068         4486 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2157344, whole genome shotgun sequence.
ACCESSION   DBNGZC010000068 DBNGZC010000000
VERSION     DBNGZC010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2157344"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="AC16G0_01205"
     CDS             <1..537
                     /locus_tag="AC16G0_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HIR5098269.1"
                     /translation="GVAVVYDSKVHGSWNALRVQTDRLHGSVKDLGERTWIRRYVDER
                     RAWLIGCADPLPRTVYRMEEFTGLIQAAKWSLSLPIRVRGVTIDEEALQGGTPVRASA
                     ESDGERILRLRTPAMQGSDVASLQRALFAAGIRIRPDRADGIFGAGTDAAVRRFQQRR
                     GLKVDGIVGPATRAALGL"
     gene            660..1058
                     /locus_tag="AC16G0_01210"
     CDS             660..1058
                     /locus_tag="AC16G0_01210"
                     /inference="COORDINATES: protein motif:HMM:NF024806.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5098270.1"
                     /translation="MKWWNRGLAYAVVLLLGLAVSVAAQDGAKPPDPRMQADAHYELG
                     VMYHERIFESLDQAITEYQTAVKLRPDYAEAHYNLALSYHTKAKLGTDDKVLYRRAVA
                     EYKLYLRYSPKGELADKAKQNIRAVEAKLR"
     gene            1151..2170
                     /locus_tag="AC16G0_01215"
     CDS             1151..2170
                     /locus_tag="AC16G0_01215"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR5098271.1"
                     /translation="MALGQACLAAGLAPRQAGAFSVFDEAGDVKIGREADPEILKKFG
                     YYDGPEIQNYVAQLGQRVAATSDTRFNFQFKVVDQPYINAMALPGGFVYVTRGMLGEL
                     NDEAALATILGHEMTHVNSRHGAKLLTKAMGAQIATLLGLGAAAASGGGSALAGVAMI
                     SSHITNYMLLGYGREYEQEADEVGLRYAHTAGYDPRRMVVFLRALRRKEMLSGQTMYH
                     GFEATHPDTSVRIAKADTMASLLVSEGGPLLVKADEYKEHLDGLTYGEAKEQRLIRVY
                     TARPGDSLASIAKEAMGNEGRRYELAGLNGLRDDAVVAPGSRLKLVFPRGESTKSLDL
                     QLKKD"
     gene            2296..3006
                     /gene="ubiE"
                     /locus_tag="AC16G0_01220"
     CDS             2296..3006
                     /gene="ubiE"
                     /locus_tag="AC16G0_01220"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HIR5098272.1"
                     /translation="MSDVVDERGAAVERMFSAIAPRYDFLNRLLSAGRDRVWRREAVR
                     ATALPAQGRLLDVCTGTADVALEATRQFPNIQVFGVDFSRPMIALGQTKVERAHLTDR
                     IRLQVAPAEALPFPDESFDAVTVAFGLRNIPDRDRGLREMARVLKAGGRAVVLEFTTP
                     PSPVFRRVYLWYFHRILPRIGGLISGHPSAYAYLPASVTDFPSPADLSSLMRTTGFSD
                     VSYQLLTMGIAAIHVGVK"
     gene            complement(3361..4215)
                     /locus_tag="AC16G0_01225"
     CDS             complement(3361..4215)
                     /locus_tag="AC16G0_01225"
                     /inference="COORDINATES: protein motif:HMM:NF025056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HIR5098273.1"
                     /translation="MPHLFISYSREDRDFVDGLIRDICEAGFEVWLDREDIRGGSQWR
                     AAITQAIHECSAFLVILSPRSVGSRNVGRELELASDNNRSILPIIYQPCEIPAEFEYH
                     FAGVQRIDFSDKSGRETPLDEVIDALKALTGTNSKKKVTPRRQPTPTAQSPTTPQPQP
                     TSVLPSFDPQTGVGTQPTPSLLQLLPGRWSIRIRHPFAMVEANLVVDLAPNGMFRGQL
                     QKPMGMTAIQGQWRATFTNQIILQGQETDGLQFAPYGAAIQINQVTPNQLMGVTSGGE
                     EVLWQRIA"
BASE COUNT          823 a         1328 c         1512 g          823 t
ORIGIN      
        1 ggcgtggccg tggtgtacga cagcaaggtg catggctcgt ggaacgccct gcgggttcaa
       61 acggaccgtc tgcatgggtc ggtcaaggac ctcggggaac ggacgtggat ccgccgctac
      121 gtcgacgagc gtcgggcctg gctgataggc tgtgccgacc cgctgcctcg caccgtgtac
      181 cgcatggagg agtttaccgg attgatccag gcagccaaat ggtccctttc cctcccgata
      241 cgcgtccgcg gtgtcaccat cgacgaagag gcactgcaag ggggaactcc cgtgcgcgca
      301 tcggccgagt ccgacgggga gcggatactc cgcctccgga cgccggcgat gcaggggtcg
      361 gacgtggcta gcctgcaacg ggcgttgttc gcggcgggta tccgcatccg gcccgaccgt
      421 gccgatggga tctttggtgc cggcaccgat gcagcggtgc ggcgattcca gcagaggcgc
      481 ggcctgaagg tggacggtat tgtcggcccg gcgacgcgcg ccgccctggg cctctaacag
      541 ccgaatgtgc tgggttattg ctgggggaga ttgggtgtgg tatgctgccc cccggtggac
      601 cagcaacagg caagcaggag gcactgtcat caacgccagg tgatcggagg attcgaacgg
      661 tgaaatggtg gaataggggt ttggcgtacg ctgtggtgct ccttctcggc ctggccgttt
      721 ctgtggcggc acaggacggc gccaagccgc cggatccccg gatgcaagcc gatgcgcact
      781 acgagctcgg ggtcatgtac cacgagcgga tcttcgaatc tctggatcag gccatcacgg
      841 agtatcagac ggccgtgaag ctgcggccgg actatgcaga ggcccactac aatttggctc
      901 tctcgtatca caccaaggcc aagttgggca cggacgacaa ggtgctctac cggcgggcag
      961 tcgccgagta caagttgtac ctgaggtaca gcccgaaggg ggagcttgcc gacaaggcaa
     1021 agcagaacat ccgggccgtg gaggccaagc ttcgatagcc attcaactcg ggaataggca
     1081 gggacgagcg atgtggtggt gttcgacgtg cacggagggt tccctggggt ggagccgtcg
     1141 acaggttctg gtggcgctcg ggcaagcgtg cctggccgcg gggctggccc caaggcaagc
     1201 cggggccttc tcggttttcg atgaggccgg ggacgtgaag atcggccgcg aggccgatcc
     1261 cgagattctc aagaagttcg ggtactacga cggcccagag atccagaact atgtggccca
     1321 gctcggtcag cgcgtggcgg ccacctccga tactcgtttc aacttccagt tcaaggtggt
     1381 ggaccaaccc tacatcaacg ccatggcgct gccggggggc ttcgtctatg tgacgcgggg
     1441 gatgctgggg gaactgaacg acgaggcggc actggcgacc atcctgggcc acgagatgac
     1501 acacgtcaac tctcggcacg gggccaagtt gctcaccaag gccatgggcg cccagatcgc
     1561 cacgctgctc ggtctgggtg cggcggctgc gagcggcggc ggcagcgcgt tggcgggcgt
     1621 cgccatgatc tccagccaca tcaccaatta catgttgctc gggtacggcc gcgagtacga
     1681 acaggaggcg gacgaggtcg gcctgcgcta tgctcacacc gcgggttacg atccgcgacg
     1741 catggtggtc ttcctccggg ccctgcggcg caaggagatg ctcagcggtc agacgatgta
     1801 ccatggtttc gaggccaccc acccggacac ctctgtgcgg atcgccaagg ccgacaccat
     1861 ggcgagtctc ttggtctccg aggggggtcc cttgctggtc aaggccgacg agtacaaaga
     1921 acacctcgac ggactcacct acggtgaggc caaagagcag cgcctgatcc gagtgtatac
     1981 ggcgagaccc ggcgacagcc tcgccagcat cgcgaaggag gcgatgggaa atgagggccg
     2041 ccgctacgaa ctggccggcc tgaacggcct tcgggatgat gccgtggttg cgccgggctc
     2101 ccgcttgaaa ctcgtctttc cacggggcga gagcaccaag tcgctggatc ttcagctcaa
     2161 gaaggactag gcggccgagg gcgaaggtag gcaccgccta aggacccggg gcgttttcgg
     2221 aggggatgac tcagggctct ggccctaagc cgtgggccgg gagtcagcgg gagggccgat
     2281 cacagggtcg agtacatgtc agatgtggtg gacgagcggg gggcggcggt cgagcggatg
     2341 ttttcggcca tcgccccccg ttatgatttt ctgaatcggc tcttgagtgc gggacgagac
     2401 cgtgtctggc ggcgcgaggc agtcagagcg accgccttgc ccgcccaggg ccggttgctg
     2461 gatgtgtgca cggggaccgc ggacgtggcc ctggaggcaa ctcgacagtt cccgaacatc
     2521 caggtcttcg gcgtggactt cagccggccc atgatcgcgc tgggccagac gaaggtcgag
     2581 cgggcacacc tcacggaccg gattcgattg caggtggccc cggccgaggc cctcccattt
     2641 cccgacgaat ccttcgatgc cgtcacggtt gcatttggcc tgcgaaacat cccggatcgg
     2701 gatcgggggc tacgtgagat ggcgagagtg ctcaaggcgg gcgggcgcgc ggtcgtcctg
     2761 gagttcacga cgcccccgag cccggtattc cgccgggtgt acctctggta tttccatcgc
     2821 atcctccccc ggatcggcgg cctgatttcc ggacacccct cggcctatgc gtacctcccc
     2881 gcgtcggtga cggactttcc ctcaccggcc gatctatcct ccctcatgcg gacaaccggc
     2941 ttttcggatg tctcctacca gctcctgacg atgggaatcg cggcgatcca tgtgggggtg
     3001 aagtgaaggg gggactccag cgatcgacgg tcacccctcg gccgcgaaag gggagcggcc
     3061 aaccggtctg gactcccagc cggggtgtcc acgatcctgg ccccaccagg cggattccgt
     3121 tgcacggtct agaaaagcag gtggcagtca tttcacacga ctcggtggag ggccgcggcg
     3181 tgtggatgaa cgtgcccctg tgtcgtcgga tccagtctag ccgggcggcg gccgagtgcg
     3241 tgcttggtct ggcgctcgcg aaggccgtgc tgacggcgga gtgacaggaa gtcctctaaa
     3301 ctccgacgca cgaagtagag ccgcgtgcat cggctcgaag gcgtggccgt tgcagcagca
     3361 ctaagcgatt ctctgccaca gaacctcttc gccgcctgag gtgacgccca tcaattgatt
     3421 gggtgtgacc tgattgatct ggatggccgc gccgtagggc gcaaactgca atccgtccgt
     3481 ctcttgtccc tgcaggatga tctgattcgt gaatgtcgcc cgccattgac cttggatggc
     3541 cgtcatcccc atcggctttt gcagttgtcc tcgaaacatg ccgtttggtg ccagatccac
     3601 aaccagattt gcctcgacca tcgcgaacgg atgtcgaatc cggatgctcc atcttccggg
     3661 caatagctga agcaaggaag gagtgggctg agtcccgact cccgtctggg gatcgaaact
     3721 gggtaacacg gaagtgggtt gcggctgtgg cgtggttggc gattgcgccg ttggggtagg
     3781 ctgacgacgc ggagtgacct tcttcttcga gtttgtgccg gttagtgctt tcagcgcatc
     3841 gatgacttca tccagtggag tctcgcgacc gctcttatca gagaagtcga tccgctgtac
     3901 accggcaaaa tgatactcga actcagcagg gatttcacat ggctgataga tgattggcag
     3961 gattgaccga ttgttgtcgg aggcgagctc gagctcgcgg ccaacgtttc ttgagcccac
     4021 agatctgggc gataggatca ccaagaatgc agaacattca tggatcgcct gggtgatcgc
     4081 tgcacgccac tgcgacccgc cccgaatatc ctctcgatcc aaccacacct cgaagccggc
     4141 ttcgcagatg tcgcggatca atccgtccac gaaatcacgg tcttcccggg aataactgat
     4201 gaaaaggtgt ggcatagagt tctacaacgg cagggtcatg gcttcgtgcc gagggtttcg
     4261 tcaactggag cggggagaaa atgtgaccct ggcccctgcc tgccgaatcg ccctagttcg
     4321 ctcccgcgac ggtcggactc cacctcccaa ctggcgtgga ggctacttca ggcgggggct
     4381 gggtgtcaac cggaaccggg gaagcatgca tgcgcacccc gctacggacg ggcaagggca
     4441 gccaggccag gtggctgcgc atcaatcagc gttgcccgag gtgcag
//
LOCUS       DBNGZC010000069         5294 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_299053, whole genome shotgun sequence.
ACCESSION   DBNGZC010000069 DBNGZC010000000
VERSION     DBNGZC010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_299053"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="AC16G0_01230"
     CDS             complement(<1..243)
                     /locus_tag="AC16G0_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098274.1"
                     /translation="MSPQPSPSDRPSPAVTLIRLLLLASLFLCGAARAGSAAEQPTDP
                     ALDQMVILGKSTGGQVTRMLVQSSGRDIWNAIFTQPP"
     gene            552..1169
                     /locus_tag="AC16G0_01235"
     CDS             552..1169
                     /locus_tag="AC16G0_01235"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR5098275.1"
                     /translation="MPDLSGLLDHWGYVAIFVFVILGNMGVPLPEETVLALAGYMVWN
                     GQLEFVAVLVIGILSAILGDNLGYWLGRRYGRGAIERYGQWVLGRPARLKSIECFVAR
                     YGFMGVFVARFVPGLRFMAGPLAGALQLPFSRFVVANLCGAASYVPVAVGVGYAIGLG
                     FGEYVEALRRIVGSVEHTVLVTALCLSAAVLLWRMLRALMEWKGT"
     gene            complement(1206..2090)
                     /locus_tag="AC16G0_01240"
     CDS             complement(1206..2090)
                     /locus_tag="AC16G0_01240"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5098276.1"
                     /translation="MGLEPIAGAAYYLPGRVNIGVLVGEGQAAFIDTGLDESAARKAV
                     QAAEQAGLRVSAVINTHAHADHCGGNAFVKKRTGASIYAPPIEAAFIQHPEFEPFYLF
                     GAAPFKAIDSKWLHATPSSVDHLLDATLSLCGLELEVIPAPGHSMNQMALATPDVAYL
                     ADTLVAAEILDRYPIQYCYDVLAHRATLDRLSGLRRAWYVGAHFSPTEDLGPLLEANR
                     TNLERTATAVLDTLNPSASTEEAVRGVAEALGVAPMDPAAYLLNAAAVKAHLSAHVRQ
                     GRIAFEIQGQRPVWRRLS"
     gene            complement(2285..3502)
                     /gene="larC"
                     /locus_tag="AC16G0_01245"
     CDS             complement(2285..3502)
                     /gene="larC"
                     /locus_tag="AC16G0_01245"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HIR5098277.1"
                     /translation="MRIGYFDCFSGASGDMILGALVDAGLPLDRLQAVIAGLVPGVEF
                     TAEQVRRGAFTGTRVHVKTDERGQPHRRLPDIQAILDRADLAEEVRRDAGRIFRRLAE
                     AEAKAHATAVEAIHFHEVGALDAIADVVGAVWGIRQLGLEHVHVSPINLGGGFVHGAH
                     GKMPVPAPGTAALLVGVPAYGSDIPVELTTPTGAAILTTLALSHGPMPLMTLQRVAYG
                     AGERDLREQPNLLRLMIGETSDSPEHDQISLLEATIDDMNPQFFEPLLDRLFEAGALD
                     AFLSPVIMKKSRPGNTLTVLAEHARADALASVILANSTTFGVRCHTVSRRKLLRDFIS
                     VSTTHGSVRVKRGWQGGRITILSPEFDDCRRLAQAAGVPIRLVYEEACEAARREAEAS
                     HLQAASHLNASSA"
     gene            complement(3602..4411)
                     /gene="larB"
                     /locus_tag="AC16G0_01250"
     CDS             complement(3602..4411)
                     /gene="larB"
                     /locus_tag="AC16G0_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HIR5098278.1"
                     /translation="MNREELLKLLSQVHSGELAPETAAEQLRHLPYEDLGFARMDNHR
                     ALRQGFPEVIFCEGKTAEQVTAIAERILARHGVLLATRASTEMFEALKTVEPRATYHP
                     LSRAIVVKPTQPPEPIGRVLVLSAGTSDIPVAEEAAVTADVLGSRVQTVYDCGVAGLH
                     RVLDQRAALEEANVLIVVAGMDGVLPSVVGGLVAKPVIAVPTSVGYGASFQGLAALLT
                     MLNSCASGIAVVNIDNGYGAGVLAHRINALGAHTDSDSDRDSGSRERKKPR"
     gene            4659..>5294
                     /locus_tag="AC16G0_01255"
     CDS             4659..>5294
                     /locus_tag="AC16G0_01255"
                     /inference="COORDINATES: protein motif:HMM:NF014841.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5098279.1"
                     /translation="MKPRAIVDPHPRTLELLFSRDDLARLKSLLSLCVWEGSRMPLHM
                     LEKHLPEACVIVGQTDLPRNRLERAKKLKAIINVEGNFQPNVDYEFCFARGIHVLSTG
                     VAFGKAVAEMALGLAIALARGIPAADRLFREGKEVYGRFSNQDSFLLSDAEVGFIGFG
                     NLGRALIRLLAPFQCRIRVYDPWLPGRWLEEFGMIPSSLEDLLTSSQMVFVL"
BASE COUNT          877 a         1633 c         1793 g          991 t
ORIGIN      
        1 gggcggttgg gtaaagatgg cgttccagat gtctcgacca cttgactgaa ccagcatcct
       61 ggtaacctgc cctcccgtgc tctttcccag aatcaccatc tggtccagcg cagggtcggt
      121 cggctgctct gccgcggagc ccgcccgcgc ggcaccacac aggaacaggg aggcgaggag
      181 gagcaggcga atcagcgtga cagcagggct cggccggtcg gatgggctgg gctgcgggct
      241 catgctcgta cctccaggga gtgaattggt cgacgtttgc accagctcag tgtgtttgac
      301 tgcggccggt ttgtcaatcc tggcctacgg ggttgagcgg tcacgggcat ccacggcgga
      361 ggccttggtg ccgacgggtg aattgagctg aatgggcact cgtgtatcca ctgcgggaac
      421 gggcactctc gatgacggaa aaccgctacg gtatcccggt gagaaatggc cagtggtgcg
      481 tgaacttcgg aggagggtcc gctgcatgaa cctgacaagg aaggcaggtc tcgcccgacg
      541 gggtctgcct catgcctgac ctgagtgggc tgctcgacca ctggggatat gtcgccatct
      601 tcgtcttcgt cattctcggc aacatggggg tgcccctccc ggaggagacg gtcctcgccc
      661 tcgccggcta catggtgtgg aacggccagc tcgagtttgt ggcggtcctg gtcatcggca
      721 tactcagcgc gatcctcggc gacaacctcg gatactggct ggggaggcgc tacgggcgtg
      781 gggccatcga gcgatacgga cagtgggtct tggggagacc tgccagactc aagtcgatcg
      841 agtgcttcgt ggcgcgctac ggtttcatgg gggtcttcgt ggctcgattc gtgccggggc
      901 tccgcttcat ggcgggtccc ctggcgggcg ccctccaact tccgttctcc cgctttgtgg
      961 tggcgaatct atgcggcgcg gcctcgtacg ttccggtggc ggtgggtgtg ggctatgcga
     1021 ttggccttgg gttcggggaa tatgtcgagg cgcttcgtcg aattgtgggt agcgtcgagc
     1081 acaccgtcct ggtcacggcc ctctgtttga gtgccgcggt gctgctttgg cgcatgctgc
     1141 gcgctctcat ggagtggaaa ggcacctgag tccggcgggg gcagctcgga acatcgcctc
     1201 tggcctcacg agagccgacg ccacacggga cgctgtccct gaatctcgaa ggcgatccgc
     1261 ccctggcgga cgtgggcgga gaggtgggct ttgaccgccg ccgcgttgag caaataggcg
     1321 gcgggatcca tgggcgcaac tcccagggcc tcggccacac ctcgcacggc ctcctcggta
     1381 ctggcagacg gattcagcgt gtccagcacc gctgtggccg ttcgctcgag attcgtccgg
     1441 ttggcctcga gaagaggccc gaggtcctcg gtgggtgaaa agtgggcgcc gacgtaccag
     1501 gcccgacgca ggccggagag ccggtccagt gtcgcccggt gcgccaggac atcgtagcaa
     1561 tattggatag ggtaacggtc cagaatttcc gccgccacca gggtgtcggc cagataggcg
     1621 acgtcgggcg tggcgagggc catctgattc atggagtggc ccggtgccgg tatcacctcg
     1681 agctccagac cgcacagact gagcgtggcg tcgagtaggt ggtccaccga cgatggggtg
     1741 gcgtggagcc acttgctgtc aatggccttg aatggtgcgg ccccgaacag gtaaaagggc
     1801 tcaaactccg ggtgctggat gaaggccgcc tcgatcggcg gcgcgtaaat cgacgcgccg
     1861 gtgcgcttct tgacgaaggc gttgccgccg caatgatcgg cgtgggcgtg cgtgttgatg
     1921 accgcggata cgcggaggcc agcttgctcc gccgcctgca cggccttccg cgcggcactc
     1981 tcatccaggc cagtgtcgat gaaggccgcc tggccttcgc ccaccaggac cccgatgttc
     2041 acccgtcctg gcaggtagta ggctgccccc gcgatcggct caagccccat ttgccgtctc
     2101 ccggaacacc cagtgatgcg tgagactgcc acccgaagag ttcgaaccgg tcctcccggt
     2161 cgccaattgc cccgttagca ggtgtcggct gtctgtttca acgggcccga cggggagagc
     2221 ctgcgtatac gctgcctgcg caccgggcgc tcgagcgggt gcagttgatc gcgattgtca
     2281 gggctcaggc gctggacgcg ttgaggtgag aggcagcctg gaggtgagac gcctctgcct
     2341 ctcgccgggc agcctcgcac gcctcctcgt agaccagccg gatgggaacg cccgcggcct
     2401 gggccagccg ccggcagtcg tcgaattccg gcgaaaggat ggtgatccgc cccccctgcc
     2461 agccgcgctt cacgcggaca ctgccgtggg tggtgctgac gctgatgaag tcgcggagga
     2521 gcttccggcg cgagacggtg tggcagcgga ccccgaaggt ggtgctgttg gcaaggatca
     2581 ccgacgccag agcgtccgcc ctcgcatgct ccgccagaac cgtgagggta tttcccggtc
     2641 gtgatttctt catgatgacg ggggagagga aggcatccag cgccccagcc tcgaacagcc
     2701 gatccagcag gggttcgaag aactgggggt tcatgtcatc gatggtagcc tccaggaggc
     2761 tgatctggtc gtgctccggc gagtccgagg tttcgccgat catcaggcgc aggaggttgg
     2821 gttgctctcg cagatcgcgc tcgccggcgc cataggccac gcgctgcagc gtcatgagcg
     2881 gcatgggacc gtggctgagg gcaagcgtcg tcaggatggc tgctcctgtg ggcgtggtga
     2941 gctccaccgg gatgtcgctg ccgtaggcgg gaacgccgac gaggagggca gccgtgccgg
     3001 gtgccgggac gggcatcttg ccatgagcgc cgtggacgaa cccgccgccg agattgatgg
     3061 gggagacgtg gacgtgctcc agcccgagtt ggcgaatgcc ccagacggcc cccaccacat
     3121 ccgcgatggc gtccagggca cccacctcat ggaagtggat cgcctccacc gccgtagcgt
     3181 gtgccttcgc ctcggcttcc gccagcctgc ggaagatccg tccggcgtcc cttcggactt
     3241 cctcggcgag atccgctcgg tccaggatcg cctggatgtc cggcaggcga cgatgcggct
     3301 gtccccgctc atcggtcttc acgtggaccc gggtccccgt gaaggcgcca cgcctcacct
     3361 gctcggcggt gaactcgacg cccggcacga ggccggcgat caccgcctga agacgatcca
     3421 ggggaagccc ggcatccacc aaggccccca ggatcatgtc cccactcgcg ccgctgaagc
     3481 agtcgaagta accgatgcgc atgtgagagt cagtgctcct gtggttgtcg gattcgcgta
     3541 tcgctgcccg ccttttcctt ggatggtttc gacagcaaca gggatagcga cagcgaaaaa
     3601 actatcgggg cttcttcctt tctctggatc cgctgtccct gtcgctatcg ctgtcggtat
     3661 gtgcgcccag cgcgttgatc cggtgggcga ggacgccggc gccatagccg ttgtcaatat
     3721 tcacgacggc gatgccggat gcgcaagaat tgagcatggt gagcaatgct gccaggccct
     3781 ggaagcttgc cccgtaaccc acgctggtcg ggacagcgat caccggcttc gctaccagac
     3841 cacccaccac cgacggcagg accccatcca tgccggccac gacgatgagc acattcgcct
     3901 cttccagggc ggcgcgctgg tccagaacgc ggtgcagacc cgcaaccccg caatcgtaga
     3961 ccgtctgcac tcggctcccg agcacgtcgg ccgtcaccgc cgcctcctcg gccactggga
     4021 tgtcggaggt tccggcagac agcaccagga ctcggccgat cggctccgga ggttgggtcg
     4081 gcttgactac gatggcgcgg ctcagaggat ggtaggtggc ccgcggctca accgtcttga
     4141 gcgcctcgaa catctcagtc gaggcacggg tggcaagcag taccccgtgt cgtgccagga
     4201 ttcgctcggc aatggccgtg acctgctctg ccgtcttgcc ctcgcagaag atgacctcag
     4261 ggaagccctg acgcaaggcg cgatgattat ccatgcgggc gaaaccgagg tcctcgtacg
     4321 ggagatgccg gagctgttcc gctgccgtct cgggcgcgag ttcgccggag tgaacttggc
     4381 tgagcaactt cagcaactct tctcgattca tggttccctg ttctcgctaa aggtgaatcg
     4441 tctggatcgg gcggcgaaaa acagtaccat gaaagcggcc cccggccaat ggctttcgcg
     4501 cattcttccg gggaatccac cacgccgggg gtggtgcatg ggtctggagc gccgatccgt
     4561 cgaggcgagc cccccgaccg tctttcccct tgactcccgg atctgcagtg atagcgttcg
     4621 aggccgggtc aaggcacggt gaaatccagg agggatgcat gaagcccaga gccatcgtgg
     4681 atccgcatcc gagaacgctc gaattgcttt tcagccggga tgacctcgca cgcctgaagt
     4741 cactgctgag cctctgcgtg tgggagggga gtcggatgcc cctccacatg ctggagaagc
     4801 acctgccgga ggcatgcgtg atcgtcgggc agaccgatct gccgcgaaac cgtttggagc
     4861 gcgcgaagaa actgaaagcc atcatcaatg tcgaaggaaa ctttcagccc aacgtggatt
     4921 acgagttctg tttcgctcga gggattcacg tgctgagcac cggtgtggcc ttcggcaagg
     4981 cggtggcgga gatggccctg ggcctggcga tcgccctggc tcgcgggatc ccggcagcgg
     5041 accggctgtt ccgagaaggg aaggaggtct atggccggtt ctcgaaccag gattccttct
     5101 tgctttcgga tgcagaggtc gggttcatcg ggtttggtaa cctgggtcgg gcgctgatca
     5161 ggctgctcgc gccgttccaa tgccgcatcc gggtgtatga cccctggctc cctggccgct
     5221 ggctggagga gttcggaatg attccctcgt ctctggagga tctcctgacc tcctcgcaga
     5281 tggtgttcgt gctt
//
LOCUS       DBNGZC010000070         1695 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_396737, whole genome shotgun sequence.
ACCESSION   DBNGZC010000070 DBNGZC010000000
VERSION     DBNGZC010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_396737"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1695
                     /locus_tag="AC16G0_01260"
     CDS             <1..>1695
                     /locus_tag="AC16G0_01260"
                     /inference="COORDINATES: protein motif:HMM:NF014598.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIR5098280.1"
                     /translation="IVAVWAGYKFYAGRMDRTVIMADPNKATPAKMYNDGVDFMPASS
                     SVLFGYQFKSIAALGPIVGPIVAVQWGWLPAVLWLILGVFFIGWIQDYTSAMLAMRND
                     GLTMGGLSYKLISPRARTILLSFLYFYLLLIMGAFAAIVAGVFSNPNLPIGFIILTLM
                     GVLAGQMTYKWRKDLVMTTVLTVVLSLVGIWLGTTGPLRAVVDWINGLNLVSYATPYG
                     PMTWPVWFWGIVALGFCYLGSVLPIWRFAQPVNYVSFWLVLMGIVGSIVGILFTMPSF
                     GDFPAFTGFMAKGIIAPGFNTPLWPILFVTIACGAISGWHSLVSTSGTARQLEKETDA
                     LPVGAGAMFTEAVLAILSVTFAVTAFGGFGGYMEKLTKGGGPGAVFADGMAKYLNVLG
                     IPTTFGGAFGRAFLVIMALTVMQLVLRFMRVASAELVGDKMPAFRNVHFGTTVGVLLT
                     LVLLWTGFWSRIWILFGGSNQLFAGLALVLITIWLAQTGKAYQWAMWPAIFMYVTTVA
                     ALIVTAWVSFQAAFTPGLKAAFVFGNLLSAVIGFALVILAIILALDAVRAFGKARSL"
BASE COUNT          242 a          525 c          542 g          386 t
ORIGIN      
        1 catcgtggct gtttgggccg gttacaagtt ctatgcgggc cgcatggatc ggacggtcat
       61 catggccgac ccgaacaaag ccacgccggc gaagatgtat aacgacggcg tggacttcat
      121 gccggccagc tccagcgtgc tcttcggcta ccagttcaag tccattgccg ccctgggacc
      181 gatcgtcgga cccatcgtcg cggtccagtg gggatggctg ccagccgtcc tctggctgat
      241 cctgggtgtg ttcttcatcg gctggatcca ggactacact tcggcgatgc tcgccatgcg
      301 gaacgacggg ctcaccatgg ggggtctgag ctacaagctc atctctccgc gggcccggac
      361 catcctcctg tcgttcctct acttctacct gctcctgata atgggggcct tcgcggccat
      421 cgtcgcaggg gtcttctcga acccgaacct tccgatcggg ttcatcatcc tgaccctgat
      481 gggtgtcctc gccggccaaa tgacttacaa atggcgcaag gatctcgtga tgaccaccgt
      541 cctcacggtc gtgctgtccc tcgtggggat ctggctgggg acgacggggc ctctccgggc
      601 cgtcgtggac tggatcaacg ggttgaatct cgtctcctac gcgaccccgt acggtccgat
      661 gacgtggccg gtgtggttct gggggatcgt ggcgctgggc ttctgctacc tgggctcggt
      721 cctgcccatc tggcgtttcg cccagccggt caactacgta tccttctggc tggtcttgat
      781 ggggatcgtc ggctccatcg tcgggatcct cttcacgatg cccagcttcg gggactttcc
      841 ggcattcacg gggttcatgg ctaaggggat catcgcaccg ggcttcaaca cgccgctctg
      901 gcccatcctg ttcgtgacca ttgcctgcgg cgccatctcg gggtggcaca gcctggtgtc
      961 cacgtcgggc acggcacggc agcttgagaa ggaaaccgat gcccttcccg ttggggcagg
     1021 cgcgatgttc acagaggctg tgctcgctat cctttcggtg accttcgccg tgaccgcttt
     1081 cggcggattt ggcggctaca tggagaagct aaccaagggt ggtggcccgg gggcagtctt
     1141 cgccgacggg atggccaagt acctcaacgt ccttggaatt ccgacaacct tcggcggggc
     1201 gttcggacgg gcgtttctgg tcatcatggc cctcaccgtg atgcaattgg tcctgcggtt
     1261 catgcgggtg gccagcgccg agttggtggg cgacaagatg ccggccttcc ggaatgttca
     1321 cttcgggacc accgtcggcg tcctgctgac cctggtgctc ctctggacgg gcttctggtc
     1381 ccgcatctgg atcttgttcg gcggctcgaa ccagttgttc gcaggcctgg ccctggtgtt
     1441 gatcacgatc tggctggccc agaccggcaa ggcgtaccag tgggccatgt ggccggcgat
     1501 cttcatgtac gtgacgacgg tcgccgccct gatcgtgacg gcctgggtct ccttccaggc
     1561 cgcgttcacg ccgggcctca aggcggcgtt cgtgttcggc aacctgttgt ctgcagtgat
     1621 agggttcgcg ctggtgatcc tggccatcat cctggccttg gatgcggtca gggcctttgg
     1681 gaaggctcgc agtct
//
LOCUS       DBNGZC010000071         2028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1277826, whole genome shotgun sequence.
ACCESSION   DBNGZC010000071 DBNGZC010000000
VERSION     DBNGZC010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1277826"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /gene="tdh"
                     /locus_tag="AC16G0_01265"
     CDS             <1..1061
                     /gene="tdh"
                     /locus_tag="AC16G0_01265"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF003808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HIR5098281.1"
                     /translation="ATGRGRSLEMMAIVQAAEGPGVELQHVPVPEPGPEEVLIRVKAA
                     AICGTDLHIAQWNAWAQHAGIRLPLVMGHEFSGEVVSIGSRVRALKPGDYVAGETHLP
                     CGECYQCRNGLQHICMRLKLFGIHRDGCFAEYTTIPEICAYRVPAALSPRVAAMLEPL
                     GTSLRAVLELDVSGGSVAVIGCGPIGLFAVACAKALGASRIIGLDVREERLALAKRLG
                     CDMSLDPRHEDATATILEATGGVGVDAIVEASGNAVALEGAFAYLRKGGRCALVGLPS
                     EPVHLNIGADVVFKEATIVGIHGREMFRTWTRMRQLLASGLLNVDPVITHEMPLEGYA
                     EGLALLAAGKGGKVILVP"
     gene            1179..>2028
                     /locus_tag="AC16G0_01270"
     CDS             1179..>2028
                     /locus_tag="AC16G0_01270"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR5098282.1"
                     /translation="MKRVGRMAGLMLIGLLVSALAGPAWGAYKAEYKMSVRTTQTSPW
                     GAGAQKFADTVREKSGGHINIKVYYNGALFADDQTKEFMLTKQGVADFALGSTINWSP
                     QLRVLNLFNLPFMFPSYEALDAVKNGAAGKMIKEQMDKFGVVFLGWGENGYRELTNRV
                     HPVVTPDDMKGLKVRAAASPIFIDTFKAMGANPVLMNWGDALSALQQGTIDAQENPVN
                     VIIIPYKIAQMHKYITVWHYAIDPLILGVSKTTWDTFTAADKRMMQQAVDEAMKWELK
                     EARDGLV"
BASE COUNT          369 a          604 c          701 g          354 t
ORIGIN      
        1 gagcgacagg cagggggcgc agcttggaga tgatggcgat cgtgcaggcc gcggaggggc
       61 cgggggtaga gctgcagcac gtacccgtcc ccgaaccggg accggaagag gtgctcatcc
      121 gggtgaaggc cgccgccatc tgtggaaccg atctgcacat cgcgcagtgg aacgcctggg
      181 cccagcatgc cggcatccgg ctgcccctgg tgatgggtca cgaattttcc ggcgaggtgg
      241 taagcatcgg aagccgggtg cgggctctga agcccggcga ctacgtcgcg ggggagaccc
      301 acctcccttg cggggaatgc taccagtgtc gaaacggcct gcagcacatt tgcatgcgcc
      361 tgaaactctt cggaattcac cgggatggat gtttcgccga gtacacgacc atcccggaaa
      421 tctgcgccta tcgggtgccg gcggcgcttt cgccgcgggt cgcggccatg ctggagccct
      481 tggggacctc gctgcgcgcg gtgctggagc tggacgtgtc gggcggctca gtggccgtca
      541 tcggctgcgg tcccatcgga ctcttcgccg tggcgtgcgc caaggctctg ggggcctccc
      601 ggatcatcgg cctggacgtt cgggaggaac ggctggcctt ggcaaaacgc ctcgggtgcg
      661 atatgagcct ggaccctcgc cacgaagacg ccacagccac catcctggag gcgacgggcg
      721 gggtgggtgt ggacgcaatc gtcgaggcgt cggggaatgc ggtggccctg gagggggcgt
      781 tcgcctactt gcggaagggc gggagatgcg cgctggttgg gctgccctcc gagcccgtcc
      841 atttgaacat cggggcggac gttgtcttca aggaggcaac gatcgtcggc atccatgggc
      901 gggaaatgtt ccgcacctgg acgaggatgc ggcagttgct ggcgagtggt ctcctgaacg
      961 tggatccggt catcacccac gaaatgcccc tggaaggcta tgcagagggc ctcgcgcttc
     1021 tcgctgccgg gaaggggggc aaggttatcc tggtcccatg agtgcaggcc gcgactcggg
     1081 cccgagtgca ttcccggctc gcgggccgtg atgagtatcc tgtccgccgg gcaaatcgtt
     1141 ttccacttgc atcctgagca atcaggggag gagacgccat gaagcgtgtc ggaagaatgg
     1201 ccggtttgat gctgattgga ctgctcgttt ccgccctggc cggcccggcc tggggcgcgt
     1261 acaaggccga gtacaagatg tccgtgcgga cgacccagac atcgccctgg ggcgccggcg
     1321 cgcagaagtt cgccgatacc gtccgggaga agtccggcgg ccatatcaat atcaaggtgt
     1381 actataacgg ggcgctcttt gccgacgatc agaccaagga gttcatgctg acgaagcagg
     1441 gggtggcgga tttcgccctt ggctccacga tcaactggtc gccccagctc cgggtgctca
     1501 acctcttcaa tctccccttc atgtttccca gctacgaggc cctggacgcg gtgaagaacg
     1561 gcgctgccgg caagatgatc aaggagcaga tggataagtt cggggtggtg ttcctgggct
     1621 ggggcgagaa cggctaccgg gagctcacga accgcgtgca ccccgtggtc acccccgatg
     1681 acatgaaggg attgaaggtg cgggccgccg cctcgcccat cttcatcgat accttcaagg
     1741 ccatgggggc caacccggtc ctgatgaact ggggggacgc gctgtccgcg ctccagcaag
     1801 gcacgattga cgcccaggag aacccggtca acgtgatcat catcccgtac aagattgccc
     1861 agatgcacaa gtacatcact gtctggcact atgccatcga tccgctcatc ctgggagtca
     1921 gcaagacaac ctgggatacg ttcaccgccg ccgacaagcg gatgatgcag caggcagtgg
     1981 atgaggccat gaagtgggag ctcaaggaag cccgggacgg cctcgtcc
//
LOCUS       DBNGZC010000072         3609 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1375352, whole genome shotgun sequence.
ACCESSION   DBNGZC010000072 DBNGZC010000000
VERSION     DBNGZC010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1375352"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..474
                     /locus_tag="AC16G0_01275"
     CDS             235..474
                     /locus_tag="AC16G0_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098283.1"
                     /translation="MEVVTLIIAVLALVLAVAAFARTGGIRDLRSQLDSVASKTETAR
                     DRTADALARLERLIRGKEKSEGGTEGGSGGAQGPR"
     gene            532..1638
                     /locus_tag="AC16G0_01280"
     CDS             532..1638
                     /locus_tag="AC16G0_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HIR5098284.1"
                     /translation="MIESTLTDLQSLLSRRISQLGLKLEGSPVERYVQMLHRELERKG
                     LKHFRPPCYLTDEWGCPSGEPIIGIPFYLADSRLARLEQEIDGLEDEREIRRYLRHEA
                     GHAFNYAYKLYETPEWRDLFGPFQRPYRDRYRPIPFDRNFVRHIEGWYAQKHPDEDFA
                     ETFAVWLTPASRWRTRYRDWPAIHKLVYVDRTARSLGNVEPPVRLASTDITTDEMHVT
                     VGEFFQSALSALTPVDVALDHDLPDFFIRRGRRKKGLRPAWEMIREHRTHLINKVEYW
                     TGVHRTVVRALVESIERAAERLQLSVAPSAESTALIELTTYTTTLCMNFLTRRSFIPK
                     GRPGKTSPQPQRGETSSRAEADDGATPNRDRPRP"
     gene            1601..2608
                     /locus_tag="AC16G0_01285"
     CDS             1601..2608
                     /locus_tag="AC16G0_01285"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR5098285.1"
                     /translation="MARLRIAIAHGPDDHPEASGPGKPTRRKSDIQEIAEVLTRAGHE
                     VFFVVVDGSRDCLVKLAAVQADLVYNLVEGFGADDTKEPSVAAYYDLLGLRYTGSGPR
                     GLTLAMDKALAKKVLQFHGVLTPRFVTHFRGRLDWAHDLEFPVIVKPLREDGSIGIGF
                     RALVSSIKELMERIDELHADFDHPVLIEQYIDGREIYVGVIGNVKPEAFPPVELDLSH
                     LPEGTPRIAGTEVKWQAGTRAYRGSKYRIPTDLPEPVVERIQAAALTSFQALQLRDYA
                     RFDFRVTSDHTPYLIEANPNPYLYSRGEFMLGARATGRTHPRVILEIVEAALSRSGAP
                     A"
     gene            2706..3167
                     /locus_tag="AC16G0_01290"
     CDS             2706..3167
                     /locus_tag="AC16G0_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HIR5098286.1"
                     /translation="MIKALIRWGLVAGVVVLLAIQLVPYGRRHTNPPVRQEPSWDSPR
                     TRELVVRACYDCHSNQTAWLWYTNVAPGSWLVQRDVDEGRRKLNYSEWDRHQKDARKA
                     ADKVEVGKMPPIYYSAFHPKAWLADGERAELIRGLANTFGRRVLGSHGKYE"
     gene            complement(3200..>3609)
                     /locus_tag="AC16G0_01295"
     CDS             complement(3200..>3609)
                     /locus_tag="AC16G0_01295"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR5098287.1"
                     /translation="VQGKVRQVVRAGLPCRDLLSQGEQAVREHRFAPYGRFVAHGIGM
                     VSHEQPDISPVNARPLEAGMVLSIETEFVHPEVGHVKIEDAVAVTEAGCEGLGDLGRD
                     WHVLPAYTPMPVPSGSQRWDEGYPAKDVKAGRQ"
BASE COUNT          663 a         1171 c         1167 g          608 t
ORIGIN      
        1 acctcgaccg ggtcctgagc cggcgcgggg gagagggggg cggcaaaggg ggatgacggc
       61 atcgcgcagc ggtcccccac ttcagccgcg ccgcgggaaa ggcgaaaggg gcccgcgaac
      121 ccggcccttc gtgcccggta cctcacgttg tctgggctga ggctgctttg atccggatcc
      181 ttcagaaagt ctcctcccca accgttcccc tctggcagag aaaggaggat tgccatggag
      241 gtcgtcaccc tcatcatcgc cgtccttgct ctcgtcctgg ccgtggcagc gttcgcccgc
      301 accggcggga ttcgcgacct gcgcagtcag ctcgattcgg ttgcctccaa gaccgagacg
      361 gcacgggaca gaacggcgga cgccctggcc cgcctggagc gtctcattcg cgggaaggag
      421 aagtccgaag gaggcacgga aggcggctcc ggtggcgccc agggcccccg atgaggcccc
      481 gattgcagaa cgggcttctg cgtggcaata taggggtgga tgtgcgtgcg tatgattgaa
      541 tcgacgctta ccgatcttca gtcgctgctt tcccggcgta tcagtcagct cggcttgaag
      601 ctggaaggct caccggtgga gcgctacgtg cagatgctcc accgcgagct ggagcggaag
      661 gggctgaagc attttcggcc gccgtgctat ctgaccgacg agtggggatg tccatccggc
      721 gagcccatca tcgggatccc cttctacttg gccgattcca ggttggcccg gctggagcag
      781 gagatcgacg ggctggaaga cgagcgggaa atccgcaggt atctccgcca cgaggcgggg
      841 catgcattca attacgccta caagctctac gagactccgg aatggcggga tctcttcgga
      901 cccttccagc ggccataccg cgatcgatat cggccgatcc cctttgatcg gaacttcgtc
      961 cgccacatcg agggttggta cgcccagaaa cacccggatg aagatttcgc cgagacgttc
     1021 gcggtctggc tgacccccgc ctcgcgatgg cggacgaggt accgcgactg gccggcgatt
     1081 cataagctcg tctacgtgga tcggacggcc cgcagtctgg gtaacgtcga gccaccagtt
     1141 cgcctggcct cgaccgatat cacgacggac gaaatgcatg tgacggtcgg ggagttcttc
     1201 cagtcggccc tgtcggcatt gactcccgtg gacgtggcgc tggatcatga cctgccggac
     1261 ttcttcatcc ggaggggacg ccggaagaaa ggactccgac ccgcctggga gatgatccgg
     1321 gagcaccgca ctcacctcat caacaaggtc gagtactgga caggcgtgca tcgaacggtc
     1381 gtgcgcgccc tggtggaaag catcgagcgg gctgcggaac gccttcagct ctccgttgcc
     1441 ccctcggccg aatccaccgc cctcatcgag ctgacgacgt acaccaccac cctgtgcatg
     1501 aacttcctca cccggagaag cttcatcccg aaagggcgcc cgggcaagac atctccccag
     1561 ccccagaggg gggaaacgtc atcccgggcc gaggcagacg atggcgcgac tccgaatcgc
     1621 gatcgcccac ggccctgacg accatccgga ggcgtcggga cctggaaaac ccactcgaag
     1681 aaagagtgac atccaggaga tcgccgaggt cttgacccgg gccggccacg aggtcttctt
     1741 cgtcgtggtg gacgggagtc gggactgcct ggtcaagctc gcggcggtgc aggccgacct
     1801 cgtctacaac ctggtggagg gtttcggcgc cgacgacacc aaggagccct ccgtggctgc
     1861 gtactacgat ctgctgggtc tccgctacac ggggtcgggg ccgcggggcc tcaccctcgc
     1921 catggacaag gcgctggcca agaaggtcct ccagttccac ggagtgctga cgccgagatt
     1981 cgtcacccac ttccgtggtc gcctggactg ggcccacgac ctcgaattcc cggtgatcgt
     2041 caagcctctc cgcgaggacg gctccattgg gataggcttc agggcgctcg tgagcagcat
     2101 caaggagctg atggagcgga tcgacgagct gcacgccgac ttcgatcacc cggtgctgat
     2161 cgagcaatac atcgacggtc gcgagatcta cgtgggggtg atcggcaacg tgaagccgga
     2221 ggcgttccct ccggtggagc tcgacctgtc ccacttgccg gaggggacgc ctcggatcgc
     2281 cggaaccgag gtcaagtggc aggccgggac gcgcgcctat cgcgggagca agtaccgtat
     2341 ccccacggac ctgcccgagc cggtggtgga acggatccag gccgcggcgc tgaccagttt
     2401 ccaggccctc cagctccgcg actatgcccg cttcgatttc cgcgtgacct ccgaccacac
     2461 cccgtacctc attgaagcca atcccaatcc ctatctctat tcgcgcgggg agttcatgct
     2521 cggcgctcgc gcgacgggcc ggacccatcc tcgggtgatt ctggaaatcg tcgaggcggc
     2581 cctctcgcgc tccggcgccc cggcttagcg aggcatgctc actttcaggc caagggcgag
     2641 gcgttcgatg cagagtggtg aagttataaa ggcctgactt catgtcagtg caggaggaag
     2701 gtatcgtgat aaaggcattg atcaggtggg gacttgtcgc aggtgtcgtc gtgctgctgg
     2761 ccattcaact ggtgccctac ggacggagac acaccaaccc tcccgtccgg caggagccgt
     2821 cctgggatag ccctcgcacg cgcgagctgg tggtccgggc ctgctacgac tgccacagca
     2881 accagacggc ctggctgtgg tacaccaacg tcgccccggg gtcctggctg gttcaacggg
     2941 acgtggacga gggccggagg aagttgaatt attcagagtg ggaccggcac cagaaggacg
     3001 cccgcaaggc ggcggacaag gtcgaagtcg gaaagatgcc gcccatctat tattcggcct
     3061 ttcacccgaa ggcctggctg gccgacggcg agcgcgccga gctcatccga ggcctcgcaa
     3121 acaccttcgg ccggcgggtt ctgggaagtc acggcaagta cgagtaaata gcggactccc
     3181 gcccggaaaa cccggccggt cattgccgcc ccgccttcac gtccttcgcc ggatagcctt
     3241 catcccaccg ctgggacccg gaagggacgg gcatgggggt gtacgctgga aggacatgcc
     3301 aatcgcggcc gagatcgccg agtccctcgc agccggcctc cgtcacggcc acggcgtcct
     3361 cgatcttgac gtggccgacc tcggggtgca cgaactctgt ctcgatcgac aggaccatgc
     3421 cagcctccag cggccgtgca ttcaccggag agatgtcggg ctgctcgtga gacaccatgc
     3481 cgatgccgtg ggccacgaac cgcccatacg gggcgaagcg gtgctcgcgg actgcctgct
     3541 ccccctggga cagcaaatca cggcaaggca gaccggcccg cacgacctgt cgaaccttcc
     3601 cctggacct
//
LOCUS       DBNGZC010000073         4771 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_6174, whole genome shotgun sequence.
ACCESSION   DBNGZC010000073 DBNGZC010000000
VERSION     DBNGZC010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_6174"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="AC16G0_01300"
     CDS             complement(<1..740)
                     /locus_tag="AC16G0_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR5098288.1"
                     /translation="MHEKLDAVERRHEDIVAKLSEPAVLADQAEFQRLAKLHADLVPV
                     VEKYRGLKKLQRELEEARALLHETIDSEMRDLAQAELEALTAKRQAAEESLQLMLLPK
                     DPADERNIILEIRAGAGGEEAALFAAELLRMYTRYAETQRWKVEMIDTSMTGIGGIKE
                     AILTVEGRGVYSCLKFESGVHRVQRVPATEASGRIHTSTVTVAVLPEAQDVDVQINPN
                     DLRIDVYRSTGPGGQSVNTTDSAVRITHL"
     gene            complement(1075..1314)
                     /gene="rpmE"
                     /locus_tag="AC16G0_01305"
     CDS             complement(1075..1314)
                     /gene="rpmE"
                     /locus_tag="AC16G0_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR5098289.1"
                     /translation="MKAGIHPAYGPALITCACGEVVHTRSTVPMLRVEICSKCHPLFT
                     GRQKLIDSEGRVERFQRKYGRKTGPAPTAEPKAEA"
     gene            complement(1422..2669)
                     /gene="rho"
                     /locus_tag="AC16G0_01310"
     CDS             complement(1422..2669)
                     /gene="rho"
                     /locus_tag="AC16G0_01310"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012645774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR5098290.1"
                     /translation="MNLAELKEKTIAELTAIARSLNVVGASGLRKQEMIFKILEAQTE
                     KNGLIFAEGVLEILPDGFGFLRAPDYNYLPGPDDIYVSPSQIRRFDLRTGDTVSGQVR
                     PPKEGERYFALLKVEAVNFENPELIKEKILFDNLTPLFPNQRFHLETTGDEISMRIMD
                     LITPRGTGQRGLIVSPPRTGKTVLLQKIANSITKNHPEVILIVLLIDERPEEVTDFQR
                     SVKAEVVSSTFDEPATRHVQVAEMVIEKAKRLTAPKRDVVILLDSITRLGRAYNTIVP
                     PSGKVLSGGVDSNALQRPKRFFGAARNIEEGGSLTIIATALIDTGSRMDDVIFEEFKG
                     TGNMELHLDRRLVDKRLFPAIDIFRSGTRREELLLTPTELNKMWVLRKVLGTMGVVEA
                     MELLQEKVRETKSNEDFLRAMNQ"
     gene            complement(3086..3733)
                     /gene="coaE"
                     /locus_tag="AC16G0_01315"
     CDS             complement(3086..3733)
                     /gene="coaE"
                     /locus_tag="AC16G0_01315"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR5098291.1"
                     /translation="MLVVGLTGGIATGKSTVAGMFAAQGAAIVDADRIAHDLQEPGRS
                     CYREIVEAFGTEILDRTGRIDRRGLGALVFANPLARRRLERIMHPAIREACRVEIRAA
                     EAAGRTVCLVDAALILETGQRHRYQKIVLVTAPEEVQVARLVQGRGLTPEEARQRLRA
                     QWPTSAKAAYADFIIDNGGDRAATETRVAVVYAALKECACGKDTGPCLPADEGER"
     gene            complement(3823..4701)
                     /locus_tag="AC16G0_01320"
     CDS             complement(3823..4701)
                     /locus_tag="AC16G0_01320"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HIR5098292.1"
                     /translation="MPKAPRLVLVDGSSCLYRAYFALPPLSNSSGTPTHAVLGFTTML
                     LKLLREEEPEALGVAFDGPGPTTRHHEFADYKAQRPEMPDAMVRQIPYVHQVLQAMRV
                     PILMIPGEEADDILGTMAVRAAAGDFTVTLITGDKDLLQVVGERITARDTMKQRTAGP
                     AEVQERYGIPPAHLPDFLALTGDSIDNIPGVPGIGDKTARDLLQRFGTLETLLERVDD
                     VPRPKLREALRAHADQARLSKRLATVRTDLCVPWRIADLIRQAPDSAALLALFRELQF
                     TRLAQQFAQGQFHIPT"
BASE COUNT          791 a         1563 c         1505 g          912 t
ORIGIN      
        1 aggtgggtga tgcggacggc cgagtccgtg gtgttgacgc tctgaccacc cggaccggtg
       61 gagcggtaga catcgatgcg caggtcgttc ggattgatct gcacatccac gtcctgggcc
      121 tcgggcagga cggcgaccgt gaccgtggag gtgtggatgc gcccgctggc ctccgtggct
      181 gggacccgct ggacacggtg tactccgctc tcgaacttga ggcagctgta gaccccgcgg
      241 ccctccacgg tgaggatagc ctccttgatt ccgccgatcc cggtcatgct ggtgtcgatc
      301 atctccacct tccagcgctg ggtctcggcg tagcgggtgt acattcggag gagttcggcc
      361 gcaaacaggg ccgcctcctc tccccccgcg ccggcccgga tctccaggat gatgttccgc
      421 tcatccgcgg gatccttggg cagcagcatg agctgcaggg actcctccgc ggcctgtctc
      481 tttgccgtca gcgcctccaa ctcggcctgg gccaggtcgc gcatctcgga atcgatggtc
      541 tcgtgcagga gggcccgagc ctcctccagt tctcgctgca gcttcttcag gccgcggtac
      601 ttttccacca ccggcaccag atcagcgtgc agtttggcca ggcgctggaa ctccgcctgg
      661 tccgccagga cggccggctc ggagagcttg gccacaatgt cttcgtgacg gcgttcaacc
      721 gcgtcgagct tctcatgcaa tgtggacatc ggatcacctt caagacactg cccctccggg
      781 catccggcgc cgggagcttc gcactgccaa aggaaaggcc agaggtaggg gacgagagga
      841 agcggagctc cggtctgccg aggacaggct agtgatggtg cccgtcgaca gcaggctggc
      901 ggtgagggtg tgcggagtgg gagtaaagcg ggcagcccag cccatcacgc agacgtgggg
      961 cccgggcgat gaatgaatgc accccggggg cggtacacct atggaacagg aggcgtgaag
     1021 gttccctgtc tcgaatcatt gggatacccc tcccgggttc tcccccagat ggggtcaggc
     1081 ctcggctttc ggctcggcgg tcggggccgg tccggtcttg cgaccgtact tgcgctggaa
     1141 ccgctccacc cggccctcac tgtcaatcag cttctgccga ccggtgaaga gcggatggca
     1201 cttggagcag atttccaccc ggagcatcgg caccgtggag cgcgtgtgca ccacctcacc
     1261 gcaggcgcag gtgatcaacg cgggtccgta agcgggatga atcccggcct tcatcgtttg
     1321 gcctccctgt tcgttttcaa gactttcggg aaactcaagc gcggtattct agtcgacttt
     1381 cccgaggctc acaagcacaa agcgctggct gcccagggcg cctactggtt cattgcccgc
     1441 aagaaatcct cgttgctctt ggtctcgcgg accttctcct gcaggagttc catggcctcg
     1501 accaccccca tggtccccag gaccttccgc aggacccaca tcttgttcag ctccgtcggg
     1561 gtcaagagca attcctcgcg ccgggtgccc gagcggaaga tgtcgatggc cgggaacagg
     1621 cgcttgtcca ccaacctgcg atccaggtgg agctccatgt tgcctgtgcc cttgaactct
     1681 tcgaagatca cgtcgtccat gcggctcccg gtgtcgatca gggccgtggc gatgatggta
     1741 aggctgcccc cctcctcgat gttccgagcc gcgccgaaga atcgcttggg ccgctgcagg
     1801 gcgttgctgt ccacgccgcc cgagagcacc ttcccgctgg gcggcacgat ggtgttgtag
     1861 gcccgaccca atcgcgtgat ggaatccagc aggatgacga cgtcccgctt gggcgcggtc
     1921 agccgcttgg ccttctcgat gaccatctct gccacctgga cgtgtcgtgt ggccggctcg
     1981 tcgaaagtgg agctcacaac ttcggccttc accgatcgct ggaagtccgt gacttcctcg
     2041 ggccgctcgt cgatcaagag gacgatcagg atgacctccg gatggttctt ggtgatgctg
     2101 ttggcgatct tctggagcag caccgtcttc ccggtccggg gtggcgatac gatcaatcct
     2161 cgctgccccg tcccccgcgg agtgatgagg tccatgatgc gcatggagat ctcgtccccc
     2221 gtggtttcca ggtggaaccg ctggttgggg aagagggggg tcaggttgtc gaagaggatc
     2281 ttctccttga tcagttcggg attctcgaag ttgaccgcct cgaccttcag gagggcaaag
     2341 taccgttctc cctctttggg gggtcgcacc tgccctgaca cggtgtcacc cgtgcggagg
     2401 tcaaaccgcc ggatttggga cggagagacg tagatgtcgt ccggtcccgg caggtagttg
     2461 tagtctggcg cccgcaggaa tccgaagccg tctggcagga tctccaggac cccttcggcg
     2521 aagattagac cgttcttctc cgtctgggcc tccagaattt tgaagatcat ctcctgcttc
     2581 cgcaggccgc tggccccgac cacgttcaat gagcgggcaa tggcggtcag ttcggcgatc
     2641 gtcttctctt tgagttcggc caggttcatc gaaacccctc tggtggaatc accctgctcc
     2701 cgctcccgga tagtccgggt ggggcgctcc gcttccacgg ctgacattgt tgtccccagg
     2761 gacatccctc tcgtaacaga agcagcttgg gctggcatcc cgtgaccggc acgggacaaa
     2821 cttctacacg gggaggtggc gcagtagttt cttgcactcg gatggctgta aattcaccta
     2881 tcggtcccga aactgtcgca ggcgattctg gccttcgaat cctcgagggc ggatatcttc
     2941 agtgggaaaa tgcgaggaca gctcgaagtt ggcgatactc tatggggcgt cgtgggcttt
     3001 gtcaagtcct tttctgccgg aggagagaaa aaacccggga tgtccatcgc gcaggacgaa
     3061 agcctcccgg cactttcggc cgcccctacc gctccccctc gtcggccggg aggcacggtc
     3121 ccgtgtcctt gccgcaggcg cactccttca gcgcggcgta gaccaccgca acccgggttt
     3181 cggttgccgc ccggtccccg ccattgtcga tgatgaagtc ggcataggcc gccttggccg
     3241 aggtcggcca ctgggcccgc aatcgctggc gcgcctcctc cggcgtcagg ccccggccct
     3301 gcacaagcct tgcgacttgg acctcctccg gtgcggtcac cagaacgatc ttctggtacc
     3361 ggtgccgctg tccggtctcc aggatcaggg cggcgtccac cagacacacc gttcggccgg
     3421 ccgcctcagc cgcccggatc tcgacccgac acgcctcgcg aatggccggg tgcatgatgc
     3481 gttcgagccg acgccgtgcc aagggattcg cgaacaccag cgctcccagg ccccggcggt
     3541 cgatgcggcc tgttcgatcc aggatttccg tcccgaatgc ctccacgatc tcccggtaac
     3601 aggaccgccc cggctcctgt aggtcgtgtg cgatccggtc agcgtccacg atagcagccc
     3661 cctgcgcggc gaacattcca gccaccgtgg atttgcccgt ggcgatcccg ccggttaggc
     3721 cgacaacgag catggaccct ggccccgcct gcccgccaat gcaataccga cgaacgacaa
     3781 cggacgaccg gtcaacgcag gcacatggcc gggtgtcgtc tgtcaggtag gaatatgaaa
     3841 ttgaccctgc gcaaactgct gggcaaggcg cgtgaactgc aattcccgaa agagcgccag
     3901 gagcgccgcg ctatccggcg cctgccggat gagatcggca atcctccacg gcacgcagag
     3961 gtccgtccgc accgtggcca gacgcttgct gagccgagcc tggtccgcgt gcgctcggag
     4021 ggcctctcgc agcttgggcc tgggcacgtc atccacccgc tccagcagcg tctccagtgt
     4081 cccgaagcgc tgcagcagat cgcgggccgt cttgtcgccg atccccggca ccccggggat
     4141 gttgtcgatg ctgtcacccg tgagggccag gaagtccggg aggtgtgccg gggggatccc
     4201 gtaccgctcc tgcacctcgg cgggtccggc ggttcgttgc ttcatcgtgt cccgggccgt
     4261 gatgcgttcc cccaccacct ggaggagatc cttgtccccg gtgatcagcg tcacggtaaa
     4321 gtccccggcg gcggcacgca ccgccatcgt ccccaggata tcgtccgcct cttcacccgg
     4381 gatcatgagg atcgggactc gcatcgcctg tagcacctgg tggacatagg ggatctgccg
     4441 gaccatggca tccggcatct ccggccgctg ggccttgtag tcggcgaact cgtggtgtcg
     4501 ggtcgtcggg ccgggcccat cgaaggccac gcccaacgcc tccggctcct cttctcggag
     4561 gagcttcagg agcatggtgg tgaaccccag gacggcatgg gtcggtgtcc cgctcgagtt
     4621 actcagcggt ggcagggcga agtaggcgcg atagagacag gagctcccat ccaccaggac
     4681 caaacgcggt gccttcggca tgtcccacgt cctcgggatg aacgaaccac gagcgccaat
     4741 gtaagacgga caaccgaaaa ctgacaaccg a
//
LOCUS       DBNGZC010000074         3575 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_984277, whole genome shotgun sequence.
ACCESSION   DBNGZC010000074 DBNGZC010000000
VERSION     DBNGZC010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_984277"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..128
                     /locus_tag="AC16G0_01325"
     CDS             <1..128
                     /locus_tag="AC16G0_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu C-terminal domain-related protein"
                     /protein_id="HIR5098293.1"
                     /translation="GDNVTMTVELIQPIAMEKELRFAIREGGRTVGAGVVSEVVE"
     gene            217..366
                     /gene="rpmG"
                     /locus_tag="AC16G0_01330"
     CDS             217..366
                     /gene="rpmG"
                     /locus_tag="AC16G0_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HIR5098294.1"
                     /translation="MREILSLACGECKRRNYSTRKNKRNTTERLELSKYCPWCRKHTP
                     HRETK"
     gene            435..510
                     /locus_tag="AC16G0_01335"
     tRNA            435..510
                     /locus_tag="AC16G0_01335"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:468..470,aa:Trp,seq:cca)
     gene            591..791
                     /gene="secE"
                     /locus_tag="AC16G0_01340"
                     /pseudo
     CDS             591..791
                     /gene="secE"
                     /locus_tag="AC16G0_01340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
     gene            963..1499
                     /gene="nusG"
                     /locus_tag="AC16G0_01345"
     CDS             963..1499
                     /gene="nusG"
                     /locus_tag="AC16G0_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HIR5098295.1"
                     /translation="MPPHHKWYVIHTYSGFENKVKQSIEQRVAALGLGDAVSQVMIPI
                     ETVVELKKGKKREASRKFFPGYVLVRMDMTDDLWYLIMNTPKVTGFVGPGVRPTPIPE
                     DQVKTIIQQVETGAERPKHKVLFSRGESVRVIDGPFSNFTGVVDEVKPDKGRLKVMVS
                     IFGRPTPVDLEFLQVERV"
     gene            1524..1961
                     /gene="rplK"
                     /locus_tag="AC16G0_01350"
     CDS             1524..1961
                     /gene="rplK"
                     /locus_tag="AC16G0_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HIR5098296.1"
                     /translation="MSKRVGKKVTAQVKLQVPAGKANPSPPVGPALGQHGVNIMEFCK
                     AFNAQTQAQEGLIIPVVITVYADRSFTVITKTPPAAVLLKQAAGIAQGSKEPNRNKVG
                     KVTRKQVEDIAKTKLPDLNAFNLEAAVKIIEGTARSAGIEVVA"
     gene            2149..2868
                     /gene="rplA"
                     /locus_tag="AC16G0_01355"
     CDS             2149..2868
                     /gene="rplA"
                     /locus_tag="AC16G0_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HIR5098297.1"
                     /translation="MAKVGKRFAKALEAVKQAVGSNGGLDLGQASALAKANAAAKFDE
                     TMDLAVRLGVDPKHADQMVRGTVVLPHGTGKKVRVLVFAKGEKEKEAREAGAEYGGAE
                     DRVEKIQGGWLDYERAIATPDVMGLVGRMGKILGPRGLMPNPKTGTVTFEVGKAVREF
                     KGGKIEFRVEKAGIIHVPFGKASFTAEQLQENAMAILEALLRAKPASSKGRYVKGISI
                     SSTMGPGIPVDANAVQALIKI"
     gene            2881..3405
                     /gene="rplJ"
                     /locus_tag="AC16G0_01360"
     CDS             2881..3405
                     /gene="rplJ"
                     /locus_tag="AC16G0_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019878838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HIR5098298.1"
                     /translation="MGRTEKATSIAELREKLDSARSAVLTDFRGLSVADLTELRTLLR
                     KSAVEYAVVKNTLAKIAVKDTSLAGLSAYLEGPTAIAISRADPVAASKVLSTWGKTRP
                     TFSVKGGMVEGKIVGPAEIAALGDLPSREVLLARMAGAFQAPLQGLVQVLAATIRSLA
                     VVLDQVRQHKEKGA"
     gene            3473..>3575
                     /locus_tag="AC16G0_01365"
     CDS             3473..>3575
                     /locus_tag="AC16G0_01365"
                     /inference="COORDINATES: protein motif:HMM:NF027644.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HIR5098299.1"
                     /translation="MAKVSVDDVLESIDSWTVLDLNKLVKGIEEKYGV"
BASE COUNT          733 a         1046 c         1250 g          546 t
ORIGIN      
        1 ccggggacaa cgtgaccatg accgtcgagc tcatccagcc catcgccatg gagaaggaac
       61 tccgcttcgc catccgcgag gggggccgga ccgtgggcgc cggggtggtg agcgaggtgg
      121 tcgagtagtc cgaaatcccg cgaacgaccc cccacgagat gggatctgcc gggtgcccgg
      181 ccggggtttc cagagggggg ctggagcaat gccatcatga gagagatcct gagcctggcc
      241 tgtggcgagt gtaagcggcg gaactattcc acccggaaga ataagcggaa caccaccgag
      301 cggctggagc tgtccaagta ctgcccgtgg tgccggaaac atacaccgca ccgggaaacc
      361 aaatgacctg cggtcgagaa gcgtgcgccc cggcctcctc agagcgggcg cggcatccgc
      421 atggctcagc gaataggcct gtagctctaa cggtagagcg gcggactcca aatccgtagg
      481 ttgggggttc gaatccctcc aggcctgcca cggattgaag agccgacagc cagagggacg
      541 ggggagcctt cgaggactgg ctgtcagatt ccggaaagga catggcgcag atgacggcag
      601 tggcggtgcg ggcgcaggtg tggtggggga ccacggttca gttcctcaaa gaggtccggg
      661 tggagatgaa gaaggtcacc tggccccagc ggaaggagat catcggctcc accgccgtgg
      721 tcatcctggc cagcttcgtc gtggcctttt tcctggggtt cgtggatctc atcctgcaga
      781 agttgctcgg gctcatcctc aaataccgta acgcactcat tcccacgtca ggacgaatca
      841 tgggcgagga cgaagcacag gccgtggtgg cgccacccgg gacgcctcag gatgcagcag
      901 ccccggccgt ggtggagacc tccggagagg tgggggggga gcagccggca gcctctccgc
      961 cgctgccgcc gcaccacaag tggtacgtca tccacacgta ctcggggttc gagaacaagg
     1021 tcaagcagag catcgagcag cgtgtggccg ccttgggcct cggcgacgcc gtctcccagg
     1081 tcatgatccc catcgagacc gtggtcgagc tgaagaaggg aaagaagcgg gaggcttccc
     1141 gcaaattctt tccgggctac gtgctcgtgc gcatggatat gaccgacgac ctttggtacc
     1201 tgataatgaa cactcccaag gtgaccggct tcgtcggccc gggggtccgg ccgacgccga
     1261 tccccgagga ccaggtgaag acgatcatcc agcaagtcga gaccggggca gagcgcccga
     1321 agcacaaggt cctgttcagc cggggcgaaa gcgtcagggt catcgacggg ccattctcca
     1381 acttcaccgg cgtggtggac gaggtgaagc cggacaaggg gcgcctgaag gtcatggtga
     1441 gcatcttcgg ccgcccgacg cccgtggacc tcgagttcct gcaggtcgag cgcgtctaac
     1501 ccaatggcaa gcgaggagcg ggcatgagca agcgtgtcgg caagaaggtg acggcccagg
     1561 tgaagctaca agtcccggca ggcaaggcca acccctcgcc accggtgggg ccagccctgg
     1621 ggcagcacgg cgtcaacatc atggagttct gcaaggcctt caacgcccag acccaggccc
     1681 aggaggggct gatcatcccc gtggtgatca cggtctacgc ggaccggtcc ttcaccgtta
     1741 tcaccaagac gccgccggcg gccgtcctgc tgaagcaagc cgcgggcatc gcccagggat
     1801 ccaaggaacc gaaccggaac aaggtcggaa aggtcacgcg caagcaggtt gaggacatcg
     1861 ccaagaccaa gctgccggac ctgaacgctt tcaatcttga ggcggcggtc aagatcatcg
     1921 agggcacggc tcgaagcgcg gggatcgaag tcgtcgcgta gtcacgcggc gcaatgaaaa
     1981 atggaaaacc gacaacggac aatgggccat cggtcagcga cattggccgg ttgtcggttg
     2041 tcagtttgaa atttgaaatt ggctgtgtcg ggcgacggtt cacctcagcc gctgcccgga
     2101 gaactttaat ttttgcgcgg tagcgcaaaa atcaggaggg agccaaacat ggcgaaggtg
     2161 ggcaagcggt tcgcgaaggc gctggaggcg gtcaagcagg ccgtcgggtc aaacgggggc
     2221 ttggacctag gccaagccag cgccctcgcc aaggccaacg ctgcggccaa gttcgacgag
     2281 accatggatc tggcggtgcg gctgggggtg gaccccaagc atgccgatca gatggtccgg
     2341 ggaacggtgg ttctccccca cgggaccggg aagaaggtcc gggtcctggt cttcgctaag
     2401 ggggaaaagg aaaaagaggc gcgggaggcg ggcgccgaat acggcggcgc cgaggatcgg
     2461 gtggagaaga tccagggggg ctggctcgac tacgagcggg ccattgccac gcccgatgtg
     2521 atgggcctgg tgggccgcat gggaaagatc ctgggtcccc ggggcctcat gcccaacccc
     2581 aagacgggca ccgtgacctt cgaggtgggc aaggccgtgc gggagttcaa ggggggcaag
     2641 atcgagtttc gtgtggagaa ggccggcata atccatgtcc ccttcggcaa ggcctccttc
     2701 acggcggagc agcttcagga gaacgccatg gcgatcctgg aggctctgct gcgggccaag
     2761 cccgcctcca gcaaggggcg gtacgtgaaa ggaatctcca tctcgtccac catgggcccc
     2821 gggatcccgg tggacgccaa cgcggtgcag gcgctgatca agatctagga ggaggtacct
     2881 gtggggcgca cggagaaggc caccagcatt gcggagcttc gcgaaaagct ggacagcgca
     2941 cggagcgcag tgctcaccga cttccgcggc ctgagcgtcg cggatctcac ggagctccgg
     3001 accctcctcc gcaagagtgc ggtggagtat gcagtcgtca agaataccct ggccaagatc
     3061 gccgtgaagg ataccagcct ggcgggactc tcggcctacc tggaaggacc cacggccatc
     3121 gccatcagcc gggcggaccc ggtggcggcc tccaaggtcc tgtccacctg gggcaagacg
     3181 cggccgacct tctcggtcaa ggggggcatg gtcgagggga agatcgtggg accggccgag
     3241 atcgcggcct tgggcgacct gccgtcccgc gaggtcctgc tggccaggat ggccggcgcg
     3301 ttccaggcgc cgttgcaggg actggtgcag gtcctggcgg caaccatccg gtccctggct
     3361 gtcgtcttgg accaggtccg tcagcacaag gagaagggcg cgtaaggccg ggctcgtcgc
     3421 gccacatgga gacgtaactc ttccgtttgg atccccgaaa gaggaggcgg caatggcaaa
     3481 ggtaagcgtg gacgatgtgc tggagtcaat cgattcctgg acggtcttgg acctgaacaa
     3541 gctcgtcaag gggatcgagg agaagtacgg cgtct
//
LOCUS       DBNGZC010000075         2441 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1278176, whole genome shotgun sequence.
ACCESSION   DBNGZC010000075 DBNGZC010000000
VERSION     DBNGZC010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1278176"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(299..391)
                     /locus_tag="AC16G0_01370"
                     /pseudo
     CDS             complement(299..391)
                     /locus_tag="AC16G0_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005576373.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(416..2149)
                     /locus_tag="AC16G0_01375"
     CDS             complement(416..2149)
                     /locus_tag="AC16G0_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HIR5098300.1"
                     /translation="MDATLELTHERVDDVPLLVGFLIQLRFPEVLDRHLPPHPLHQGL
                     SNGWLITVWIAYILSQADHRKSHVQEWVDSLHHTLETLIGQPLRPVESSDDRLTLVLK
                     RLSDPAVWHELETDLWHTQCDVYAVPPVERVRLDATTSYGYHAINDDGLMQLGHSKDH
                     RDDLPQIKLMAAAAEPSGLFLAGDVHPGHAADDPLYLPLYRRVRALLGQTGLLYAGDC
                     KMAAIETRAEIAANGDYYLTRLPLTGEVAAQFAAWVEAAITGEAAATMVEIRREDELI
                     GRGYEFVRSQTTVVEKTEYTWIERVQVIRSEALAQSQAAALERRLEKAEAAVRGLTPP
                     PGPGKTQFTTGWELERAVAAVLSEQDVEGLLEVNWEREETSRTRYVGRGRGGPNRPKM
                     TTWSVRYQITTVLRNEAAIRKRVERMGWQVEVTNLPAARLSLGEALLAYRGGWFVERL
                     FHLLKDQPLGIRPLYVQRDDQIQGLTHLVTLALRVLTLFEVLVRCGQERCGEKLPGLY
                     PGQAKRVTDRPTAKRVLEAISRARITLTQVESVGEVRWHLPALPVLVKRVLGYLGLSE
                     AVYTQLVINSV"
BASE COUNT          462 a          770 c          750 g          459 t
ORIGIN      
        1 gctcagctcg actggtgcag attctcggcc ctatgctgcg gtggcgaggg cggaaaacat
       61 gacctcgcgg atcggggccg ggagttcttg aatggctcgg tcctcatccg cctgtgcaaa
      121 aacccaatcc gaccacagcc aacgagggtg tgaccgacga atgatgtaat tgaagttggc
      181 ctggaagccg gacttatgga aaaccggcag cttctccagg gaaaaatggc ctctcctgag
      241 acgtgcgttt cgcagcctgt ttttcggctt acatcattcg tcggtcacac ccttgacgac
      301 acagatcccg caggatcgga ccgatctgcc gcctcagcct tccgtagaag accttccggc
      361 ggaatttcgg tatgatcacg acgtggtact gccctttcgc gtattgaaag agtgctcaga
      421 ctgaatttat gactagctga gtgtatactg cctcggacag tcccaggtag cccagcaccc
      481 gcttcaccag cactggcagg gctggcagat gccagcgaac ctcaccgacg ctttccacct
      541 gggtcagcgt gatccgggct cgggaaatcg cctccaacac ccgcttcgct gtcgggcggt
      601 ccgtgacccg cttcgcctgg ccgggataca accccggcag cttctcaccg caccgctctt
      661 gcccgcaacg caccaagacc tcgaacagcg tcaacacccg caacgccagc gtcaccagat
      721 gggtcaagcc ctggatttgg tcgtctcgct gcacgtacag cggccgaatc cccaggggtt
      781 ggtccttcaa cagatgaaac agccgctcca caaaccagcc cccacggtag gccagcagcg
      841 cctcccccag cgacagccgc gccgccggga gattggtcac ctcgacctgc cagcccatcc
      901 gctccacccg ctttcggatc gccgcctcgt tgcggagcac cgtggtgatc tggtagcgga
      961 cgctccaagt ggtcatcttg gggcggttag ggccaccgcg gcctcgaccc acgtaccgag
     1021 tccggctggt ctcttcacgc tcccagttca cctccaacag cccctccaca tcctgctcgc
     1081 tcaggaccgc agccacggcc cgttccaact cccagccggt cgtgaactgg gtcttgcccg
     1141 gccccggcgg tggcgtcaag ccgcgtacgg ccgcctcggc tttctccagc cgtcgctcca
     1201 gcgccgccgc ttggctctgg gccaacgcct cggagcggat gacctgcacc cgctcgatcc
     1261 aggtgtactc tgtcttctcc accacggtgg tttgcgaccg cacgaactcg tatcccctgc
     1321 cgatgagctc atcctcgcgc cgaatctcga ccatcgtcgc cgccgcctca ccggtgatcg
     1381 ccgcctccac ccaggccgcg aactgggccg cgacctctcc ggtcaagggc agccgggtca
     1441 ggtagtaatc cccattggcg gcgatctcgg cccgtgtctc gatggctgcc atcttgcagt
     1501 cgccagcgta gagcagcccg gtttgtccca gcagcgcgcg gacccgccga tacaagggga
     1561 ggtacagcgg gtcgtcggcc gcatggccgg gatgaacatc gccggccagg aacaagccac
     1621 tgggttcggc cgcggcggcc atcagcttga tctggggcaa gtcgtcccgg tgatctttgc
     1681 tgtgacccag ttgcatcaac ccgtcgtcgt tgatggcgtg gtagccgtag ctggtggtgg
     1741 cgtcgaggcg gacccgctcg accggtggca cggcatagac gtcgcattgg gtgtgccaga
     1801 ggtcggtttc cagttcatgc cagaccgccg ggtcactgag gcgtttgagg accaaggtga
     1861 ggcggtcgtc ggaggactcg acgggccgaa ggggctgtcc gatgagggtc tcgagggtgt
     1921 ggtggagact gtcgacccac tcctggacat gggacttgcg gtggtcggcc tgggagagga
     1981 tgtaagcgat ccagacggtg atgagccagc cgttggagag accttgatgg aggggatgtg
     2041 gggggagatg gcggtcgagg acctcgggga atcgcagttg gatcaggaag cccaccaaca
     2101 gggggacgtc atcgacgcgt tcgtgagtca attccagggt ggcgtccatg gtggtactcc
     2161 gacgagggct gcggggaaag cctcgttgta ccgccaggga cggccggacc ggaagatcca
     2221 agaggaggaa cgtgaacata ggaacgctgc acagaatggg ctaaaacata attagcggtt
     2281 tactaaacat ttgctacgcg aaagcggagc ttgtgtacaa agattccacc aattttcaag
     2341 acgcccaccg ccctgagaac tgagaagtat agtatttttt ttgttgagag gtggaatagt
     2401 ccctgctatg atcgcaggaa gcgccccttg atgggagaac c
//
LOCUS       DBNGZC010000076         4643 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_397245, whole genome shotgun sequence.
ACCESSION   DBNGZC010000076 DBNGZC010000000
VERSION     DBNGZC010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_397245"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="AC16G0_01380"
     CDS             <1..276
                     /locus_tag="AC16G0_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR5098301.1"
                     /translation="QVRNVRIFGAGFSLAAVAAGRLDAYWEQSAKPWDVAAGALLVAE
                     AGGRVTDGAGRPFGVEHSTILASNGRVHRRLVGALNGRPASPHTRRT"
     gene            276..1427
                     /locus_tag="AC16G0_01385"
     CDS             276..1427
                     /locus_tag="AC16G0_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5098302.1"
                     /translation="MPTSPPLTLGVAVLGYAGVAKAHLNALKKVPYIFWPSPVRFRLV
                     GIGGRSPAGVEDAARRYGFEYGTTEWQRLVEDPRIGLFINCAPNDAHAAPCLAAVGQG
                     QHLLCEKPMARDAREARRMWEGVRRAPVRHQVSYNYRFIPAVRLAREMIRAGDLGDII
                     HFRTRYTDDTLSDPATPHTWRQEKRRAGTGAVGDIASHVLDLGRFLVGEVRSVAGLSR
                     IVVPRRPVKPGSRQTKVVDVEDLYEGVLEFAGGAVGTLEVSTFCPGRKNYLTFEVNGT
                     RGSLGFNLERLNELEVFRVESRGRRDRGFKTVLVTDSDHPYGGRWWPPGHILGWEHTF
                     VHQLQHLVTAIAGGGTVGPLAADFHDGYVNAVLCDALVKAARTGKRLRV"
     gene            1653..2795
                     /locus_tag="AC16G0_01390"
     CDS             1653..2795
                     /locus_tag="AC16G0_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 periplasmic-binding domain-containing
                     protein"
                     /protein_id="HIR5098303.1"
                     /translation="MRIRPTTSLGFCLTLVLAFALLMAPTPAPAAAPTELVIADSQSG
                     ANFQAYWQKYVIPAVKQALGVNLKYLVSSDAEQIQKAKAWKPGQGDVHIIFPKSIATW
                     VASGVPLETLTTSNIPNSAKIEPHFLKTDEGVDLQSKGVLYWRTSYALIYNSQNIKNP
                     PKSWKEYYDRRAEFKGHIGLIRPDAKSSSAWRQRLTFLHAFLKDKMTQPIGQLEKDPA
                     FVDAWNKLKDFTKYAKLPLPAEPPNMFEDFNAGDTWISLYAQDYSLWSARQGTMPPTI
                     KAVYPEEGADEVGTGYLAVPANIPPEYKDVALKVVNYLLSDDQQIRLITTMWQYPGTD
                     ITEQVPPIVWEIVPKLDVLTKTAIPRERIRKDMIDYIKGHAKELIP"
     gene            2882..3742
                     /locus_tag="AC16G0_01395"
     CDS             2882..3742
                     /locus_tag="AC16G0_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098304.1"
                     /translation="MTALWRRHSSAMVTLFLLLPALVGFFGLFFYPMLLTLVLSFRPE
                     GQTAGWTLQNYTTFLSDPDGRWVILLTFILALGATVLSVLLSVPLSLILREKLRGHRF
                     YRLMIIVPLVIPGLIGALGLLLFFGTRGWFNLFLLQFAPFVAPPLAVNYTIHGLILFY
                     VWLYFPYTCVTTMSALESLDQGIEEAGAVSGANRWQVLRHIVLPLITPGILAGSVLTF
                     MAAFGAFSVPLVAGGNYRPLAVEIYKEISVPIPGHWSAASAIAVVMGALQVVFLTLYM
                     RFVRKMGGPQ"
     gene            3739..4575
                     /locus_tag="AC16G0_01400"
     CDS             3739..4575
                     /locus_tag="AC16G0_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098305.1"
                     /translation="MTLLRAYLRGLWDGRLVLGYTLLLSLSLFILAPFGMIMLRSVGK
                     GWFGRLWLPPQLTLEWYRWAVEVGNIGEVMKNTLIIGAIAVILSTVIGIFAGWAFGRR
                     KLPGKEVLMAMILLPRMIPPITYALGVAKIFYTLDLVDTHLGVALAHVSVCAPYAILV
                     LSATFEGLDERVLEAAGVCGANTLKRFFYVVLPLIMPGILASMLFSFTHSYNEFTLTL
                     LTYGPHTVTLPVRTYLAVGDGYWEITSAMAVIMVVPSLLILAIIQRRAKPEKLIGGFK
                     GA"
BASE COUNT          773 a         1569 c         1464 g          837 t
ORIGIN      
        1 caggtgagaa acgtgcggat cttcggggcg ggcttctccc tggccgccgt agccgcaggc
       61 agactggatg cgtactggga acagagcgcc aagccttggg acgtcgccgc gggcgccctg
      121 ctggtcgccg aggcaggcgg ccgcgtcacc gatggtgccg gccgtccctt cggcgtggag
      181 cacagcacca tcctggccag caatggccgt gtccaccgcc gcctggtcgg cgccttgaac
      241 ggccgaccgg cttcccccca cacccggagg acctgatgcc cacttcaccc cctctcacgc
      301 tcggcgtcgc ggtgctggga tacgccggcg tggccaaggc ccacctcaac gcgttgaaga
      361 aggtgcccta catcttctgg ccctcgccgg tccgcttccg gctcgtgggg atcggcggcc
      421 gctcgcccgc cggtgtggag gacgccgccc gccgctacgg cttcgagtac gggaccacgg
      481 agtggcagag gctcgtggaa gacccccgga tcgggctctt catcaattgc gcgcccaacg
      541 atgcccacgc cgcgccgtgt ctcgccgcgg tgggacaggg ccagcatctc ctgtgcgaga
      601 agcccatggc gcgggatgcg cgcgaggccc gacgcatgtg ggagggggtg cgccgcgccc
      661 ccgtccggca ccaggtgagc tacaactacc gtttcattcc cgcggtcagg ctggcgcggg
      721 agatgatccg ggccggggac ctgggcgaca tcatccactt ccgcacgcgc tacaccgacg
      781 acaccctgag cgacccggcc acccctcaca cctggcgcca ggagaagcgg cgcgccggga
      841 ccggcgccgt gggcgacatc gcctcccacg tcctggacct ggggcgcttt ctggtggggg
      901 aagtccggag cgtggcgggt ctttcccgga tcgtggtgcc tcgccgtccg gtgaaaccgg
      961 gttcccgcca gaccaaagtc gtggacgtcg aagacctcta cgagggggtc ctggagtttg
     1021 ccggcggtgc cgtggggacc cttgaggtct ccaccttctg ccccggccgc aagaactacc
     1081 tcaccttcga ggtgaacgga acgcgtggga gcctcggctt caacctggag cggttgaatg
     1141 aactggaggt cttccgcgtc gaatccaggg gcaggcgcga ccgggggttc aagaccgtcc
     1201 tggtgaccga cagcgatcat ccctatggcg gcagatggtg gccccccgga catatcctgg
     1261 gctgggagca taccttcgtc caccagctcc agcacctggt gacggcgatc gctggcgggg
     1321 gaacggtcgg gccgctggcc gcggacttcc acgacgggta cgtgaatgcg gtcctgtgtg
     1381 acgctctggt caaggcggcc cggacgggga agcgcctccg ggtatagaaa ccacaaggga
     1441 gagctatgga ccacaatccg gcacacggcc catcgctggg ggctgaaatc ggatggctga
     1501 agctcaccct tgacctgcag agagagaagt gagacgcttt ggacattcag ccatgatttc
     1561 gctggaaagg gggtgatgtt tggagcagca gtacagtgtg taagccgcgc gtatggcacg
     1621 atgtgctttc tcattccgga aggagagcgg cgatgagaat tcgacccacg acaagtctgg
     1681 gcttctgcct caccctggta ctggcgttcg ccttgctgat ggcgccaacc cccgcaccgg
     1741 ctgcggcgcc gacggagctg gtcatcgccg acagccagtc gggggcgaac ttccaggcct
     1801 actggcagaa gtacgtgatc ccggccgtca agcaggccct gggcgtgaac ctgaaatacc
     1861 tggtcagcag cgacgccgag cagatccaga aggccaaggc ctggaaaccg ggccaaggcg
     1921 acgtgcacat catcttcccc aagtccatcg ccacctgggt cgcctcgggc gtgcccctgg
     1981 aaaccctcac cacctcgaat atcccgaact ccgccaagat cgagccccac ttcctgaaga
     2041 ccgatgaggg cgtggacctg cagagcaagg gcgtcctcta ctggcggacc tcctacgccc
     2101 tgatctacaa cagccagaac atcaagaacc cgcccaagtc ctggaaggag tattacgatc
     2161 ggcgggcgga gttcaagggc cacatcggct tgatccgccc tgacgccaag tccagctcgg
     2221 cgtggcggca gcggctcacc ttccttcacg ccttcctgaa ggacaagatg acccagccca
     2281 tcggtcaatt ggagaaggat ccggccttcg tcgacgcctg gaacaagctc aaggacttca
     2341 ccaagtatgc caagcttccg ctgccggcgg agcccccgaa catgttcgaa gacttcaacg
     2401 ccggcgacac ctggatctcg ctctacgccc aggactattc cctgtggtcg gcgcgccagg
     2461 gaaccatgcc ccccaccatc aaggccgtct atcctgagga gggcgccgat gaggtcggca
     2521 cgggctacct tgccgttccg gccaacatcc cgccggagta caaggacgtc gctctcaagg
     2581 tcgtcaacta cctcctgtcc gacgatcagc aaatccgcct gatcaccacc atgtggcagt
     2641 atcccggcac ggacatcacg gagcaggtcc ccccgatcgt gtgggagatc gtccccaagc
     2701 tggacgtcct gaccaagacc gcgatcccgc gcgagcggat ccggaaggac atgatcgact
     2761 acatcaaggg gcatgcgaag gagttgattc cttagctcac gcagccaaac acgtcgcggc
     2821 ctaacgggtg ggggcgggga tacccgcccc ctccttttgc gccgcagcgc gaagggagag
     2881 catgaccgct ttgtggcgca ggcattcctc ggccatggtg accctgttcc tgctcttgcc
     2941 cgccctggtc gggttcttcg gcctcttctt ctaccccatg ctgctcaccc tggtcctgag
     3001 ttttcgcccg gaggggcaga cggccgggtg gacgttgcag aactacacca ccttcctgag
     3061 cgacccggat ggacggtggg tgatcctcct cacgttcatc ctggccctgg gggccacagt
     3121 cctgtccgtg ctcctgagcg tcccgctctc gctgatcctg cgagagaagc tccggggaca
     3181 ccggttctac cgcctgatga tcatcgtccc cctggtgatc cccggtctca tcggagccct
     3241 cgggctgctc ctgttcttcg ggacgcgggg ctggttcaat ctgttcctcc tgcagttcgc
     3301 gccctttgtg gcgcccccgc tggcggtcaa ctacaccatc catggcctga tcctgttcta
     3361 cgtctggctc tacttcccct acacctgcgt caccaccatg tcggcgctgg agtcgctgga
     3421 ccagggcatc gaggaggccg gggccgtctc gggggcgaac cgctggcagg tgctgcgcca
     3481 catcgtcctc cccctcatca cacccggcat cctggccggg tccgtcctca ccttcatggc
     3541 ggccttcggg gccttcagcg tgccgctggt tgcggggggc aactatcgcc ccctggcggt
     3601 cgagatctac aaggagatct cggtgcccat cccgggtcac tggtcggccg ccagcgccat
     3661 cgccgtggtc atgggcgccc tgcaggtggt cttcctgacg ctctacatgc gcttcgtgcg
     3721 gaagatggga ggcccgcaat gacgctcctt cgggcgtacc tgcggggcct gtgggacggg
     3781 aggctcgtcc tggggtacac cctgctcctg agtctcagcc tgttcatcct ggcccccttc
     3841 gggatgatca tgcttcgctc ggtgggcaag gggtggttcg gtcgtctgtg gctgccgccc
     3901 cagctcaccc tggaatggta ccgatgggcc gtggaggtgg gcaatatcgg cgaggtcatg
     3961 aagaacaccc tgatcatcgg ggccatcgcg gtgatcctca gcaccgtgat cggcatcttc
     4021 gcgggctggg ccttcggccg gcgcaagctg ccgggcaagg aggtgctgat ggccatgatc
     4081 ctcttgcccc ggatgatccc gcccatcacc tacgccctgg gcgtggccaa gatattctac
     4141 accctggacc tggtggacac tcatctcggg gtggccctgg cccacgtctc ggtctgcgcc
     4201 ccctatgcca tcctggtcct ctccgccacc ttcgagggtc tggacgagcg cgtcctggag
     4261 gcggccggcg tctgcggcgc caacaccctc aagcggtttt tctacgtggt cctgcccctc
     4321 atcatgccgg gcatcctggc ctccatgctc ttctcattca cccattccta caacgagttc
     4381 acgctgacgc ttttgaccta cgggccgcac accgtgacgc tgccggtccg aacctatctg
     4441 gcggtgggcg acgggtactg ggagatcacc agcgccatgg cggtgatcat ggtggtcccc
     4501 tccctgctca tcctggcaat catccagcgg cgggccaagc cggagaagct gatcggcggg
     4561 ttcaagggag cgtgaggcat gcccgatgcc atgatcgaat tccgcgaggt gagtaagcga
     4621 tacgacaccc tggttgccaa tga
//
LOCUS       DBNGZC010000077         4228 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1669527, whole genome shotgun sequence.
ACCESSION   DBNGZC010000077 DBNGZC010000000
VERSION     DBNGZC010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1669527"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            862..2772
                     /locus_tag="AC16G0_01405"
     CDS             862..2772
                     /locus_tag="AC16G0_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5098306.1"
                     /translation="MYKHIYVPVDNSEHSNAAIELAVVLGKAFGATLVGSHVYAARMH
                     DYRFRQMEYTLPPEYQDEEELEKQRRIHDSLITMGLQLISDSYLEVLTQKAEAAGLTC
                     KKVMMDGKNYAELVRDIRQSAYDLVVMGALGMGAVKQTSLGSVCERTIRRISTDVLVV
                     KSVTSPDAVSGAPILVGIDGSPQSYAGLQAAMALGRVFHSPVEAVAVYDPVLHYTVFH
                     SLASVLTAKAARVFKFKEQEQLHEEVIDTGLAKIYQSHLEVARQVAHEAGVDLRISML
                     DGKAFERILRHASQREPWLLVLGRVGVHSDDAMDIGATTENLLRLAPCNVLITSRKFY
                     PPMDVKAEASIAWTPEAEARMERVPGFVKAMARTAVLRFALERGHSVITNSVIEQVMD
                     IFMPSRTADKTQDLALELAVEAIRAGRGPVYICRTCGHTVRDAEPVLCVVCGADRARF
                     EKLDSVTVEALAASEGVLQEEETFDGLTLRWTEEANRVLRLVPSGYIRRRAKARIEKA
                     ARVQALQVIGKDLAWPIAAEVIEDHKGMQDRDTLTPEQHLLRATRATGRAEDLFTWTQ
                     DAEARLNRVPEGYMRTMTKKRVEEIAAERATREITLEIVEAGIEAGKQMMAKLLEAYN
                     QTGKPASPVPQP"
     gene            2769..4157
                     /locus_tag="AC16G0_01410"
     CDS             2769..4157
                     /locus_tag="AC16G0_01410"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5098307.1"
                     /translation="MTAFLPYTVSWNLTQRCNQQCAHCYLSAFPHASQTEELTTEECI
                     RVMEEMAGVNPDLLLILTGGEPFLRKDLFDLAGQAVQRGFSVVLGTNGYLLREKQARL
                     MRQHGIQGASISLDSTDSFRHDAFRQFRGSWEGAIRATEALRTEGLPFSLHMSVTEWN
                     VGEIPAMIDLARQLGAQVLNLFFLVRTGRGEGLTDIPAEEYERVLTEVARAQVDAPAD
                     SPRWAHESGDPEESWGSELGRTGGLVIRAKCAPFFRRILYGLDPASVLLRNFAEGSCP
                     AGRHYCRIMPNGDVTACPYMPVVAGNVRQTAMGDLWRDASLFQDLRGGRLAGRCGSCE
                     FSRICGGCRCRAYATFGDYLAEDPACAYQPGAYGGRVIELPTHQTFGIPAEPTLAWSV
                     EAQARLKGIPGFARGMVVAGVERYARNRGVAVITPGLMQEVREQMGGRFPLFRRERGE
                     GRTEQYRMSNTE"
BASE COUNT          798 a         1298 c         1416 g          716 t
ORIGIN      
        1 ctcccgaccg tattgataga gcgccaaccc tggaggaggg accaacgtgc tcaggatgtc
       61 ctccaaattt cggctaggag ccatttccta tcatcggccg gggttggtaa ggacgggaac
      121 gggacccgct gccgaagggg accgcatggt ggcagggaat cggaatatct gaccacgggt
      181 ggtagaggaa ggtccgggga aattcgccga gggacgttcg tcgcacggga agcccctttg
      241 actcgtggtg gttgagtatc tctccctggc cgcgcaatgt ttacctgcta acacaggggt
      301 gtcaagcccc taactcgccc caaacttgcg gtcctgcttt tcacgtatgg tccatctcac
      361 tcacatcgca gcccccgctt gggcctatcc gcaagcgggg tgtggggccg ctggcgcttg
      421 ccccggagat ccctgacacc ggaaagcctc cgaccgtgga tgtcctgagc agggaagggg
      481 ccatgcgaac cgagcgcaca gccgcctgac aagaatccct gcgcctggag atttttccgc
      541 cgaagcaccg aaataggact tgaccgctcg gatttactat ggcagttctt ccttgccaag
      601 agcgccggag cttgcgagca gcagttgggg agcgtcgagg gcgacgatcc ggaaggttcg
      661 acgcgaagag tacggaccac gtgctcgatg cgccccgtca catagttcca tccgacgcca
      721 gtgcctcagg gcgaacccgc gcgctagctc aggaccctat gagcacgctc tcgtctcagg
      781 ggtggagtgg cgccgatgcc cacctcgcgg tcacatctta aagatggccc gacacctatg
      841 cccaccgggg cggaggcctg catgtacaag cacatctacg tccccgtcga taactcggaa
      901 cattcgaatg cggccatcga gctggccgtg gtgctgggga aagccttcgg ggccacgctg
      961 gtgggcagtc acgtttacgc ggcccggatg cacgattacc ggttccggca gatggagtac
     1021 acgcttccgc ccgagtacca ggacgaggag gagctggaga agcagcggag gattcacgac
     1081 tccctgatca ccatggggct ccagctcatc tccgattcct acctggaggt gctgacccag
     1141 aaggcggagg ccgccgggct cacctgcaag aaggtgatga tggatggcaa gaactacgcg
     1201 gagctggttc gagacatccg gcagtcggcc tacgatctgg tggtgatggg ggctctggga
     1261 atgggggcgg tcaagcagac cagtctgggc agcgtctgcg agcggacgat ccgccgcatc
     1321 agcacggacg tcctggtggt gaagagcgtc acgtcacccg atgcggtttc cggggcgccc
     1381 atccttgtcg gcattgacgg cagcccccag tcctatgccg ggttgcaggc ggccatggcg
     1441 ctgggccggg ttttccactc cccggtggag gcggtggcgg tctacgatcc cgtcctgcac
     1501 tacaccgtct tccacagtct ggcctccgtg ctgacggcga aggcggcccg ggtgttcaaa
     1561 ttcaaggaac aggagcagct ccacgaggag gtcatcgaca ccggcctggc caagatctac
     1621 cagtcgcatc tcgaggtggc ccggcaggtt gcgcatgagg cgggtgtgga cctcaggatc
     1681 agcatgctgg acggcaaggc cttcgagcgg atcctccgcc acgccagcca gcgggagccc
     1741 tggctgctgg tcctgggccg ggtcggggtg cacagcgacg atgccatgga tatcggcgcc
     1801 accacggaga acctgcttcg cctggccccc tgcaatgtcc tgatcaccag ccggaagttc
     1861 tatcccccca tggatgtcaa ggccgaggcc agcatcgcct ggacacccga ggcggaggcc
     1921 cgcatggagc gggtgcccgg cttcgtgaag gccatggccc ggaccgcggt cctgcggttt
     1981 gccctggaac gcggccactc ggtgatcacc aactccgtaa tcgagcaggt gatggacatc
     2041 ttcatgccga gccgaacggc ggataagacc caggacctgg ccctggagct ggcggtggag
     2101 gcaatccgag ccggccgcgg tccggtgtac atctgccgca cctgcggcca cacggtccgg
     2161 gatgcggagc cggtcctgtg cgtggtctgc ggggcggaca gggcgcggtt cgaaaagctg
     2221 gatagcgtga cggtggaggc gctggccgcc tccgaaggcg tcctccaaga ggaggaaacc
     2281 tttgatggcc tcacgctccg ctggaccgaa gaggcgaacc gggtcctcag gctggtcccc
     2341 tcgggataca ttcgccgacg tgcaaaggcc cgcatcgaga aggcggcacg agtgcaggca
     2401 ctccaggtca tcggcaagga cctggcctgg ccaatcgcgg cagaggtgat cgaggatcac
     2461 aagggaatgc aggaccgtga cacgctgacc ccggagcaac acctgctgcg cgccacccgt
     2521 gccaccggtc gagccgaaga cctcttcacc tggacccagg acgcggaggc tcgcctgaac
     2581 cgggtgcccg aggggtacat gcggaccatg accaagaagc gggtggagga gatcgcggcg
     2641 gagcgcgcga cgcgagagat taccctggag atcgttgagg caggtatcga ggcgggaaag
     2701 cagatgatgg ccaagcttct cgaggcttac aaccagaccg ggaagccagc ttcccccgtg
     2761 ccccaaccat gacggcattt cttccgtata ccgtctcctg gaacctgacc cagcgctgca
     2821 accagcagtg cgcccactgc tacctgagcg ccttccccca cgccagccag acggaggagc
     2881 tgacaacaga ggaatgcatc cgggtcatgg aggagatggc gggagtcaat ccagatctct
     2941 tgctaatcct cacgggagga gagcctttcc ttcggaagga cctctttgat ctggcaggtc
     3001 aggcggtgca gcggggcttc agcgtggtcc tcggcaccaa cggatacctg ctccgggaga
     3061 agcaggcgag gctaatgcgg caacacggca tccagggggc gagcatcagc ctggactcca
     3121 ccgactcttt ccgccacgac gcttttcggc aattccgggg atcttgggag ggggcgatcc
     3181 gggccacgga ggccttgcgt accgaagggc tccccttctc cctgcatatg agtgtcaccg
     3241 aatggaatgt gggtgagatc ccggcgatga tcgatctcgc caggcagctc ggggcccagg
     3301 tcctgaacct gttcttcttg gtccgcacag ggcgtggaga agggctcaca gatattccgg
     3361 ccgaagagta cgagcgcgtc ctgacggagg tcgcccgagc gcaggtggat gcaccggccg
     3421 attcgccacg atgggcccac gagagcggcg acccggaaga atcgtggggg agtgagctgg
     3481 gtcggaccgg ggggctggtg atccgagcga agtgcgcccc atttttccgg aggatcctgt
     3541 atggactgga tccggcatcc gtgttgctcc gcaatttcgc cgagggaagc tgccccgcgg
     3601 gacggcatta ctgccggatc atgcccaacg gggacgtgac ggcctgcccc tacatgcccg
     3661 tggtggcggg caatgtgagg caaaccgcca tgggcgacct gtggcgagat gcgagcctct
     3721 tccaagatct gcgcgggggg agacttgcgg gccgctgtgg ctcctgtgag ttctcccgga
     3781 tctgcggcgg gtgccgctgc cgcgcctatg ccacctttgg cgattacctg gcggaggacc
     3841 ctgcctgcgc ctaccagcca ggcgcctacg gcggtcgagt catcgagctt ccaactcatc
     3901 agaccttcgg gatccccgcg gagcccacac tcgcctggag cgtggaggcc caagctcggc
     3961 tcaagggtat ccccggcttc gcccggggga tggtggtagc aggcgtggag cgatacgccc
     4021 gcaatcgcgg ggtagctgtc atcactcctg gcctgatgca ggaagtgcgc gaacaaatgg
     4081 gcggacggtt cccgcttttt cggcgggagc gaggcgaggg acgaacagag caatatcgaa
     4141 tgtcgaacac cgaatgacga atctcgaacg gggacggcat cctggggatg cttcgcctcc
     4201 gcgttcttca ttcgacaccc ggaattca
//
LOCUS       DBNGZC010000078         2681 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1474000, whole genome shotgun sequence.
ACCESSION   DBNGZC010000078 DBNGZC010000000
VERSION     DBNGZC010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1474000"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="AC16G0_01415"
     CDS             <1..528
                     /locus_tag="AC16G0_01415"
                     /inference="COORDINATES: protein motif:HMM:NF013961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HIR5098308.1"
                     /translation="KKASPVGSSTYCLTFKTGNCTDFESLWASLARAKGIPTQIVYGS
                     FLKPELRDRDEDQSYHCWAMFYAPGLGWLPHDVAVADLYKADIPITTTNAKLVRLTTP
                     DGTFGADPAKVNYFFGNLDERRVVWSHNRDLTMNPKQDGEPVNALPKAYIEVDGKVLP
                     ETSGWMRKLTYKERT"
     gene            574..1185
                     /locus_tag="AC16G0_01420"
     CDS             574..1185
                     /locus_tag="AC16G0_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013379.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR5098309.1"
                     /translation="MAEPGEPGDSLRTDTVLLFLERAQGVRCVGCGANVCGHEAVMSL
                     AMGFKSGPRCLSCLAAGLAREPRELRDHLFAYVDHHECLRAGWDWASRQEGFDPESPP
                     ACLWPAGEGVPHVADDTNDRPQPRTSSQSVPDAEWDAGDAGCGDLVLELRMRLQSMKP
                     GEILKLTARDPGAPEDLPAWCRLTGHSLLSSQHPEYWIERKEG"
     gene            1190..1552
                     /locus_tag="AC16G0_01425"
     CDS             1190..1552
                     /locus_tag="AC16G0_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HIR5098310.1"
                     /translation="MAGKFCVSLTSAKDNRDKATVAFVVANAAVASDKDTLVFLSTEG
                     VHLSQKGYADDIREEGFAPLKELMENFVKAGGKIYVCSPCFKRRKLDEGNLVPGATIV
                     GGAKLVEFLADGSPCITY"
     gene            1757..2074
                     /locus_tag="AC16G0_01430"
     CDS             1757..2074
                     /locus_tag="AC16G0_01430"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR5098311.1"
                     /translation="MRGRGPGLRQRPAPDHPRCHGAARRGIEIQNLEVSLKARADNIL
                     VFLGIEDSGHPGLRGIEGSLYVEADAEDDVLQALWEETQLRCPVTQSLVRQVPVNISL
                     KRA"
     gene            2074..>2681
                     /locus_tag="AC16G0_01435"
     CDS             2074..>2681
                     /locus_tag="AC16G0_01435"
                     /inference="COORDINATES: protein motif:HMM:NF013847.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin-B12 independent methionine synthase"
                     /protein_id="HIR5098312.1"
                     /translation="MSSWPRYPVTVVGSWPRPSWLLDAMKRKRAELRAMQDEATLLAI
                     KYQEDAGVDIVSDGGQRRDNFYSFICDRVDGIRLMSMAELLEYVEDKAAFENLLRALD
                     VPAFAIKNPTVVGKLKPRGPLVLDDFRYLRQHTKKPVKVTLPGPYLLNRSTWVKNLSE
                     AAYPTRESLAEDVVDILRTELRALADAGADIVQFDEPVLTELV"
BASE COUNT          486 a          849 c          880 g          466 t
ORIGIN      
        1 aagaaagcat cgccggtcgg gagcagcacc tattgcctga ccttcaagac cggcaactgc
       61 accgacttcg agtctctctg ggcctcgctg gcccgggcca aggggatccc gacccagatc
      121 gtctacggct ccttcctgaa gcccgagctg cgcgatcggg atgaggatca gagctaccat
      181 tgctgggcca tgttctatgc gcccggtctc ggctggctgc cccacgatgt ggccgtcgcc
      241 gatctgtaca aggccgacat tcccatcacc acgacgaatg ccaagctggt tcgtctcact
      301 acccccgatg gcacctttgg cgctgacccg gcgaaggtca actacttctt cgggaacctg
      361 gacgagcggc gggtggtctg gtcgcacaac cgggacctga ccatgaaccc gaagcaggat
      421 ggcgagcccg tcaacgccct gcccaaggcc tacatcgagg tggacgggaa agtcctcccg
      481 gaaacctcgg gatggatgcg aaagctcacc tacaaggaac gcacgtaggc ttcgggggca
      541 gcctgccctc gcgcgattat gggccagact cccatggctg aaccaggcga accgggcgac
      601 tcccttcgca cggatacggt cctgctgttc ctggaacgcg cgcagggcgt gcgctgcgtt
      661 ggctgcggtg ccaatgtctg cggccatgag gcagtaatgt ccctggccat gggattcaag
      721 agcgggccgc ggtgcctgtc ctgcctggcc gcggggctgg cccgcgagcc acgggagctg
      781 cgggatcacc tgttcgccta cgtggaccat cacgagtgcc tgcgggcggg ctgggattgg
      841 gccagccggc aggaagggtt cgatcccgag tctccccccg cctgtctctg gcctgcgggg
      901 gagggggttc cgcacgtggc cgacgatacg aatgatcgcc cccaaccaag gacctcctct
      961 caatcggtgc cggatgccga gtgggatgcc ggtgatgcgg gctgtgggga tttggtcttg
     1021 gagctccgga tgcgcctcca atccatgaag cctggagaga tcttgaagct cacagcccgg
     1081 gacccgggcg ctcccgagga ccttccggcg tggtgccggt tgaccggaca cagcctgctg
     1141 tcctcgcagc atcccgagta ctggatcgag cgaaaggaag ggtgaccaaa tggccgggaa
     1201 gttctgcgtc agcttgacct ccgccaagga caaccgagat aaggcaaccg tggccttcgt
     1261 cgtggccaac gccgccgtcg cctccgacaa ggacacactg gttttcctga gcacggaagg
     1321 ggtccatctt tcccagaagg gctacgccga cgacatccgg gaggaggggt tcgccccctt
     1381 gaaggagctc atggagaact tcgtgaaggc gggcggcaag atatacgtct gctccccttg
     1441 cttcaagcgg cggaagctgg acgaggggaa tctcgtcccg ggcgcgacca tcgtcggcgg
     1501 ggccaagctg gtggagttcc tggccgacgg cagcccctgc atcacttact gacccggatc
     1561 cttcgcgaac gcgttcgcga agtatccggg aatgaccaaa tcccaatggc caatgaccaa
     1621 cggcggatgc ccatcccgtc tccatccgat aactcgcact ctgcgccctt catcattggt
     1681 cattggaaat ttgtcattta tctttttggg ggagggccat gactgctgag gggaacctcc
     1741 cgcaggccga cgcggtgtgc gagggcgggg acctggactg cggcagcggc ctgctcctga
     1801 tcatccgcga tgccatgggg ccgctcgccg cgggatcgag attcagaacc tggaggtatc
     1861 gctcaaagcc cgggcggaca acatcctggt gttcctgggg atcgaggata gcgggcaccc
     1921 cggcctgcgg gggatcgagg gctcgttgta cgtcgaggcc gatgcagagg acgacgtgct
     1981 ccaagccctc tgggaagaga cgcaactccg ctgcccggtc acgcaatccc tggtccgcca
     2041 ggtgccggtc aacatctcac tgaagagggc atgatgtcat cttggccgcg gtatcccgtc
     2101 acggtggttg gaagctggcc gcgtccgtcc tggctcctgg acgcgatgaa gcggaagcgc
     2161 gccgagttac gggcgatgca ggacgaggcg acgctcctgg cgatcaagta ccaggaagac
     2221 gccggggtgg acatcgtctc cgacggcggg cagcgccggg acaactttta ctccttcatc
     2281 tgcgatcgcg tggacggcat ccgcctcatg tccatggcgg agctgctgga gtacgtcgag
     2341 gacaaggcgg ccttcgagaa tctgctccgc gcgctggacg tgccggcctt cgccatcaag
     2401 aatccgaccg tggtggggaa gctgaagccg cgcggtcccc tggtgctgga tgacttccgc
     2461 tacctgcggc agcacaccaa gaagccggtc aaggtgacgc tccccgggcc ctacctgctg
     2521 aaccgctcga cgtgggtcaa gaacctttcg gaggcggcct accccacccg agagtccctg
     2581 gcggaggacg tcgtggacat cctccggaca gagctgcgcg ccctggccga cgcgggagcc
     2641 gacatcgtac agttcgacga gccggtgctc accgagctgg t
//
LOCUS       DBNGZC010000079         2244 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2647384, whole genome shotgun sequence.
ACCESSION   DBNGZC010000079 DBNGZC010000000
VERSION     DBNGZC010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2647384"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(530..1099)
                     /locus_tag="AC16G0_01440"
     CDS             complement(530..1099)
                     /locus_tag="AC16G0_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HIR5098313.1"
                     /translation="MGQTLAEPRPTSPLPSPTVARNLERIAWLMDRAIKIPGTNISLG
                     LDALLGLLPVGGDVLTGFVQAGLVLVALKHYRVPRAVAARMMGNVLLDIAVGAIPLLG
                     DMFDVFFKANTANLKLLAPYRRNRETPEVILAPERESAPLPRVRDRRSVDVRSMGTPW
                     RFLLPIAVVLVAALTLLLIGLITIVRWLM"
     gene            complement(1227..1493)
                     /locus_tag="AC16G0_01445"
     CDS             complement(1227..1493)
                     /locus_tag="AC16G0_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR5098314.1"
                     /translation="MIGSLIGWIVLGLVAGALARLLHPGQDVMGVASTIVLGVLGSLL
                     GGGAAYLLKLGTSPYEPGGWILATVGALVLLAFGWFGSRSRATV"
     gene            1927..>2244
                     /locus_tag="AC16G0_01450"
     CDS             1927..>2244
                     /locus_tag="AC16G0_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HIR5098315.1"
                     /translation="MSAGIAWGSLLWIAGLHVGALLAFLPASFTWQALAVCLALHWLT
                     GGIGICMTYHRLLTHRSFATRPWWLEYALTAIGCCASEGGPIGWVADHRKHHAHSDDE
                     HDVH"
BASE COUNT          437 a          687 c          728 g          392 t
ORIGIN      
        1 atcacatgcg aagacgtgga ggaagaaaca ccggggcgtc aaattcctgc ggctgtagaa
       61 gtagcaatgg atactgctcc gaaaatcccg ggcgggattc ggtgatgggc gcgacaaaac
      121 cccttacccc ccaggaggca caggggggta acaccgttcg caggggggcg tgcttctgtg
      181 gtgccgaacc cgggattagg gcacagactt cggcgcgccg tagagaccaa caccgacaga
      241 ccaccggaag cgggcggcga gggacggcgc gtggggggac attctgctcc gcgtgctccc
      301 cggggatccg ggggcgacaa ttcagggtcg accgggaaaa actttttctg gccggaaagc
      361 gacgtcgggc gtggaacgct cttcactttg gcgctaacgt tcgcggtggt gttcgccacc
      421 ccctcggatc gtgagccgag cctgacgaga ctacccgaga gcgatacaag gtcccagagg
      481 gacttgagat gagcccagtg gcgtcttgtt ggtacggctt cgagatcgtt tacatcagcc
      541 agcggacgat ggtgataagc ccaatgagca ggagagtcag ggcggcgacc aggaccacgg
      601 cgatcggcag gaggaaacgc cagggcgtcc ccatcgaacg cacatcgaca ctgcgacggt
      661 ccctcactct gggcaacggg gcactttccc gctccggagc caggatgact tcgggagtct
      721 cgcgattccg ccggtacggt gccagaagtt tcaagttcgc ggtgttcgct ttaaagaaca
      781 cgtcgaacat gtcgccgagc agcgggatcg cgccgacggc gatgtcgagc agaacgttgc
      841 ccatcattcg ggccgccact gccctgggta cgcggtagtg cttgagagct accagcacga
      901 gcccggcctg gacgaacccg gtcagcacgt cgccgccgac cggcagcagg cccagcaggg
      961 cgtcgagccc gagggagatg ttcgtaccgg ggatcttgat cgcccggtcc atcaaccagg
     1021 caattcgctc caggttgcgg gctaccgtag gggacggtaa cgggctcgtc ggcctcggct
     1081 cggcaagtgt ctggcccatg gggtcttcgg tgcgcgtttt ctggtgaggg cttcaagagt
     1141 gacgaggcgc tggagtggag agatgaactg ggctggtcat gacaggtcgt gaccggctca
     1201 gtcgtcatgc tggatgcacc cgacgcttac acggtagccc gcgagcggga gccaaaccag
     1261 ccgaaagcca gcaggacgag ggcgccgact gtggccaaga tccagccgcc cggctcgtaa
     1321 ggtgaggtgc cgagcttcag caggtaggcg gccccgccgc ccaggagcga gccgaggacg
     1381 cctaggacga tggtcgaggc gacacccatc acgtcttggc cggggtgtag caggcgggcc
     1441 agggcaccgg cgacgaggcc caggacgatc caaccgatca gggagccgat catgagaatc
     1501 ctctctttgg tatgtgcgat caggattcac tcgtcccggt ttagagcggt tgacgcatgg
     1561 cttgcgcaca cgcgagaggc tgtgcaatga accccccttg tgaagggggg cgaggggcca
     1621 gccaccgatt tcaccccgaa gtctgtgcgc aagccatgcg tcaaccgctc tagctcccga
     1681 gccgggtgac atcagggaaa tagaacaaga actgatcacg caaacgataa gccaaaagat
     1741 cggtttccgt atcaatacac tgtggtattt tctatgaaat caactttaga ttctcctccg
     1801 gcacatctct tgcaccgtat ccctagcatc tggttttccc gcattatggc tccggggctt
     1861 cggaaacaaa gactcccgcc ttcctctgtt cccacggtgg atatgccgtc tacgaccatc
     1921 cggcccgtgt cggccgggat tgcctggggt tccctcctct ggatcgcagg gctgcacgtg
     1981 ggggccctgc ttgcgttcct ccccgcctct ttcacctggc aggccctggc cgtttgcctc
     2041 gccctgcatt ggctgaccgg cggcatcggc atctgcatga cctaccaccg cctcctgacc
     2101 caccgcagct tcgcgacccg gccctggtgg ctggaatacg ccctgacggc catcggctgc
     2161 tgcgcctcgg aaggggggcc gatcggctgg gtggccgatc atcgcaagca ccacgcccat
     2221 tcggacgacg agcacgacgt ccac
//
LOCUS       DBNGZC010000080         3133 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_886862, whole genome shotgun sequence.
ACCESSION   DBNGZC010000080 DBNGZC010000000
VERSION     DBNGZC010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_886862"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="AC16G0_01455"
     CDS             complement(<1..970)
                     /locus_tag="AC16G0_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR5098316.1"
                     /translation="MAVFVYSGRTRGGQTLTGEMEAANREAVVAKLRTQQIIATSVRA
                     KAREIVIPGFGGGVTEKDIVVFTRQFATMIDAGLPLVQCLEILASQQENKVFKKTLMD
                     IRQSVEGGSTFAAALKQHPKVFTSLYANMVEAGEAGGILDTILNRLAQYMEKAMSLKK
                     KVKSAMVYPSTIITVAVVVVIFLLIFVIPTFKSMFEGFGATLPLPTQIVLEMSRIVRF
                     YFLYGVAVIAALIFGLKWWYGTPGGKMAIDTFLLKTPVFGILIRKVAVAKFTRTLGTL
                     ISSGVAILDGLDITARTAGNKVVEAAVLKTRASISEGKTIAEPLKES"
     gene            complement(989..2077)
                     /locus_tag="AC16G0_01460"
     CDS             complement(989..2077)
                     /locus_tag="AC16G0_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR5098317.1"
                     /translation="MPTLHELLKEMVDKGASDLHITTGVPPMIRLHGHITPIGSEALS
                     AAETKSMAYSILTEVQKQKFEAQKELDLSFGVKGMSRFRANVFVQRGAVAVAIRTIPY
                     KIFSFEELGLPKVVSDLCERPKGLILVTGPTGSGKSTTLATMIDKINSEKEEHIVTIE
                     DPIEFVHSHKKCVVNQRELHADTLSFTAALRSVLREDPDKVLIGEMRDLETIEAALTI
                     AETGHLTFGTLHTNSAVQTINRIIDVFPPHQQPQIRAQLSFVLEGVLCQMLLPKANGQ
                     GRVLVLEVMVPNAAIRNLIREDKLHQVYSMMQAGKEKSGMQTMNESLVQHYQRRNLTL
                     DQVLSASTQPDELQNMLQRSGGSQLTRR"
     gene            complement(2118..>3133)
                     /locus_tag="AC16G0_01465"
     CDS             complement(2118..>3133)
                     /locus_tag="AC16G0_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR5098318.1"
                     /translation="RLKAAITSRIKIMSQLDIAERRLPQDGRIKIKLGGKEMDYRVST
                     LPTLFGEKAVLRLLDKGNLQLDMTKLGFDQQALTDFESGLLMPFGMVLVTGPTGSGKT
                     TTLYSALNRLNTTETNIMTAEDPVEFNLGGINQVQTKAEIGLTFAAALRSFLRQDPDI
                     IMVGEIRDYETAEIGVKAALTGHLVLSTLHTNDAPSTISRLLNMGVEPFLVAASTNVI
                     LAQRLARRVCQGCKEVTPIPQQALVNIGFSPEEAKTVKPVKGKGCMACSETGYKGRVA
                     LYEVLLIKENIKEAILQGASAMELRELGRSNGMKTLREAGLQKIREGMTTIEEILRVT
                     TGQ"
BASE COUNT          521 a          963 c          996 g          653 t
ORIGIN      
        1 ccgactcctt caggggctcg gcgatggtct tcccctcgga gattgaagcc cgggtcttca
       61 agaccgccgc ttccaccacc ttgttgccgg ccgtccgggc cgtgatgtcc agcccgtcca
      121 ggatggccac gccgctgctg atcagggtcc ccagcgtccg ggtgaacttc gccacggcca
      181 ccttccggat caggataccg aagaccggcg tcttcagcag gaaggtgtcg atggccatct
      241 ttcccccggg cgtcccgtac caccacttga gcccgaagat aagggccgcg atgaccgcca
      301 cgccgtacag gaagtagaat cgaacgatcc gcgacatttc caggacgatc tgggtgggaa
      361 ggggcaatgt ggcaccgaac ccctcaaaca tggacttgaa tgtagggatc acgaagatca
      421 ggaggaagat caccacgacc accgccaccg tgatgatggt ggagggatag accatggcag
      481 acttcacctt cttcttcaag ctcatggcct tttccatgta ctgggccagc cggttcagga
      541 tcgtgtccag gatcccgccc gcctccccgg cctccaccat gttggcgtag agggaggtga
      601 agaccttagg atgctgcttc agggcggcgg cgaaggtgga gccgccctcc acggactgcc
      661 tgatgtccat cagcgtcttc ttgaagactt tgttctcctg ctgggaggcc aggatttcca
      721 ggcactgtac caggggcaga cctgcgtcga tcatggtggc gaattggcgg gtgaacacca
      781 cgatgtcctt ctcggtcacc ccgccgccaa accccgggat gacgatctcc ctggccttgg
      841 cccgcacgga ggtggcgatg atctgctgcg tgcgcagttt cgccaccacg gcctcgcggt
      901 tggctgcctc catctctccg gtgagggtct gcccgccccg ggtgcgtccg ctgtagacga
      961 agaccgccat agattcctcc tcccgctgtt agcgtcgggt caattggctg cccccagacc
     1021 gctggagcat gttctgaagt tcgtcgggct gcgtcgaagc cgacaggact tggtccagtg
     1081 tgagattccg gcgctggtag tgctggacca acgattcgtt catggtctgc atgccggact
     1141 tctccttacc ggcctgcatc atgctgtaga cctgatgcag cttgtcttcg cggatcagat
     1201 ttcggattgc cgcgttgggc accatgacct ccagcaccag tacacgcccc tgcccgttcg
     1261 ccttggggag gagcatctgg cacagcaccc cctccagcac gaaggagagt tgggcccgga
     1321 tctgcggctg ctggtgcggg ggaaacacgt cgatgatgcg gttgatggtc tgcacggccg
     1381 agttggtgtg cagggtcccg aaggtcaggt ggccggtctc ggcgatggtg agcgccgcct
     1441 cgatggtctc caggtcccgc atctcgccga tcagaacctt gtccgggtcc tcgcggagca
     1501 ccgacctcag ggccgccgtg aaggacaggg tgtcggcgtg cagttcacgc tggttcacca
     1561 cgcacttctt gtgcgagtga acgaactcga tggggtcctc gatggtgacg atatgctctt
     1621 ccttctcgct gttgatcttg tcgatcatgg tcgccagggt ggtggacttg cccgagcccg
     1681 tggggccggt caccaggatg agccccttgg gacgctcgca caagtccgag accaccttcg
     1741 gcaatcccag ctcctcgaag gaaaaaatct tgtagggaat cgtgcggatg gccacggcca
     1801 ccgcgccccg ctggacgaag acgttggccc ggaagcggct catgcccttc acgccaaagg
     1861 acaggtcgag ctccttctgg gcctcgaact tttgcttctg cacctccgtg aggatgctgt
     1921 aggccatgct cttggtctca gcggcgctca gcgcttccga cccgatggga gtgatgtgac
     1981 cgtggaggcg gatcatgggg gggacgcccg tggtgatgtg gagatccgag gcccccttgt
     2041 ccaccatctc tttcagcagt tcgtgtaagg ttggcatgcc gtggctctct ctcccgattc
     2101 tcctccggac gccggggcta ctgaccagtg gtgacgcgca ggatttcctc gatggtggtc
     2161 atgccttcac ggatcttctg caggccggcc tcgcgaaggg tcttcatgcc gttggatcgt
     2221 cccagctctc gcaattccat ggcggaggct ccttgcagga tggcctcctt gatgttctcc
     2281 ttgatcagca agacctcgta cagggccacc cggcccttgt agccggtttc actgcaggcc
     2341 atgcaaccct tgcctttgac cggcttgacg gtcttggcct cctcggggct aaagccgatg
     2401 ttcaccagcg cctgttgagg gatgggggtg acctctttgc acccctggca aacccgacgc
     2461 gccagccgct gggccaggat gacgttggtc gaggcggcca ccaggaacgg ctccaccccc
     2521 atgttcagga ggcggctgat ggtgctgggg gcgtcgttgg tgtggagcgt ggacagcacc
     2581 aggtggccgg tgagggctgc cttgaccccg atctccgccg tctcgtagtc gcggatctcg
     2641 cccaccatga tgatgtccgg atcctgccgc aggaacgacc ggagggccgc ggcgaaggtg
     2701 aggccgattt ccgccttggt ctggacctgg ttgatgcccc ccaggttgaa ctccaccggg
     2761 tcctcggcgg tcatgatatt ggtctcggtc gtgttcagac gattcagggc ggagtagagg
     2821 gtggtcgtct ttcccgaccc ggtgggcccg gtgacgagga ccatgccgaa gggcatgagc
     2881 aggccacttt cgaaatccgt cagagcctgc tggtcgaatc ccagcttggt catgtccagt
     2941 tggagattcc ctttgtcgag aaggcggagg acggccttct cgccgaaaag ggtggggagg
     3001 gtggagacgc ggtagtccat ctccttgcct cccaacttga tcttgatgcg accgtcctgg
     3061 ggcaggcgcc gctcggcgat gtccaattgg gacatgatct tgatgcgaga ggtgatggcc
     3121 gccttcagcc gga
//
LOCUS       DBNGZC010000081         2521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_104612, whole genome shotgun sequence.
ACCESSION   DBNGZC010000081 DBNGZC010000000
VERSION     DBNGZC010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_104612"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="AC16G0_01470"
     CDS             complement(<1..486)
                     /locus_tag="AC16G0_01470"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR5098319.1"
                     /translation="MRFQPETLAAIQAAEAAGRILLRRFGRENAVRFKGAKDPVTAAD
                     VEAERRIRTRLARRFPRIGFLGEEGGGVEGPDGRWIVDPLDGTLAFIAGLPFFAVCIA
                     LERRRRVEAGVLLLPRLGELFVAERGRGATLNRRPIHVSRTTRLRDAVISLWHDDTVW
                     GN"
     gene            complement(483..1553)
                     /locus_tag="AC16G0_01475"
     CDS             complement(483..1553)
                     /locus_tag="AC16G0_01475"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013486085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5098320.1"
                     /translation="MDKAYVELVRLSKRFGRVTAVGDVSLEVPEGQITTLLGPSGCGK
                     TTLLRLIAGFFPPDTGEVRIKGERVDHLPPQRRSTAIVFQDYALFPHLSVFENVAYGL
                     RRRRIRTPEITRRVRDMLRFLGLEAQEAAPPLRLSGGQQQRVALARALVVEPDVLLLD
                     EPLSNLDAKLRTRVRTELKEIQQSLGKTTIFVTHDQEEALSISDRVAIMDAGRIRQVG
                     TPIQVYTRPADRFVADFVGIANFLPGTVREVTAEGLVVDSPLGILTAQPRPAVRQGHR
                     VTILLRPEALRLTLTPEGGGLSARIRSTSFLGGTRRHRVQVGELELVVDEAFSISGEE
                     PGRETWLHVDAERLHLLPGEAA"
     gene            complement(1546..>2521)
                     /locus_tag="AC16G0_01480"
     CDS             complement(1546..>2521)
                     /locus_tag="AC16G0_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098321.1"
                     /translation="PLLATEAWYRIDGWGDLRGATLLAATLLPPAVLLFLAERYWVGR
                     RTYAVIGGRGSSLDRPPLPGLLKWGLLGFCGLLAGLILSLYFGVVAGAFTRTWGVDWS
                     PTLDQWRVAVTKLDHIRNSLVFAGWAGLLATVLATLTAYLVDQKAFRAGRALDALCVL
                     PAAVPGVFFGIGYLLVFNRPGVPLSGTALVMILAFAFHNLPFSYQIARAGLAQIHQNL
                     ADAAADLGAFRFRILWDVHLRLIFPACVAAWTTAFVNSVTNLSIAIFLVGGGNQVATF
                     SILGLIGDNRLEAASAVTTALLFLTLAVVAAAWRLSRGARVIPGVLGG"
BASE COUNT          426 a          860 c          900 g          335 t
ORIGIN      
        1 gttcccccag accgtgtcgt cgtgccagag ggagatcacg gcgtcgcgga gccgggttgt
       61 ccgtgagacg tggatgggac gacgattgag ggtagccccc cgtccccgct ccgcgacgaa
      121 cagctcgccc aggcggggga ggaggagcac gccggcctcg acccgcctcc ggcgctccag
      181 cgcgatgcag accgcgaaga atggcaggcc ggcgatgaag gcgagggtgc cgtccaacgg
      241 gtccacgatc cagcgcccat ccggtccctc caccccgccg ccttcttcgc ccaggaaacc
      301 gatccgcggg aagcgccgcg ccagcctggt ccggatgcga cgctccgcct cgacgtcggc
      361 tgccgtcacg ggatccttgg cccccttgaa gcgcacggca ttctcgcgac cgaatcgtcg
      421 caggagaatc cgtccggcgg cctcggccgc ctggatcgcc gccagggtct cgggttggaa
      481 tctcatgccg cttcccccgg cagcaggtga agccgctcgg catccacgtg aagccacgtc
      541 tcccgcccag gttcttcccc cgagatcgag aaggcctcgt ccaccacgag ctccagctcc
      601 cccacctgga cgcgatggcg ccgcgtcccg ccgaggaagc tcgtggaccg gatgcgggcc
      661 gagaggcctc cgccttccgg tgtgagcgtc aggcgcaagg cctctgggcg caagaggatg
      721 gtgactcgat ggccttgcct cacagccggc cgcggctggg cggtcaggat ccccaggggg
      781 gagtctacca cgaggccctc ggcggtgacc tcccggaccg tccccggcag gaagttggcg
      841 atccccacga agtcagccac gaatcggtcg gcggggcggg tgtacacctg gatcggggtt
      901 cccacctggc ggatgcgtcc cgcatccatg atggccaccc ggtccgagat ggacagggcc
      961 tcctcctggt catgggtcac gaagatggtg gtcttcccca gggactgctg gatctccttg
     1021 agctccgtcc gcacccgcgt ccggagcttg gcgtccaggt tgctcagagg ctcgtccagg
     1081 agcagcacat ccggctcgac gaccagggct cgggccaggg ccacgcgctg ctgctgtccc
     1141 ccggacaggc ggagcggagg ggccgcctcc tgcgcctcca ggccgaggaa gcgcagcatg
     1201 tcccggaccc gccgcgtgat ctccggcgtg cggatgcggc ggcgtcgtaa gccgtaggcc
     1261 acattctcga agacgctgag atggggaaag agcgcgtagt cctggaagac gatggccgtg
     1321 ctccggcgct gcggcggcag gtgatcgacc cgctccccct tgatccgcac ctcgccagtg
     1381 tccgggggga agaagccggc gatgagccgc agcagtgtgg tcttgccgca gccgctgggc
     1441 cccaggagcg tcgtgatctg accctcggga acctccaggg agacgtcgcc gaccgccgtc
     1501 acgcgtccaa agcgcttgct caggcgaacc agctcgacgt aggccttatc caccgaggac
     1561 tcccgggatc acgcgggcgc ctcgagacag gcgccaggcg gcggccacca ccgccagggt
     1621 gaggaacagg agggccgtgg tcacggccga ggcggcctcg agacggttgt cgccgatgag
     1681 acccaggatc gagaacgtcg ccacctggtt ccctcccccc accaggaaga tagcgatgct
     1741 caggttcgtg acgctgttga cgaaggccgt ggtccaggcg gcgacgcacg cagggaagat
     1801 cagccggagg tgcacgtccc acaggatgcg gaagcggaag gcccccagat cggccgcggc
     1861 gtcggccagg ttctggtgga tctgggccag gccggcccgc gcaatttgat agctgaaggg
     1921 caggttatgg aaggcgaaag ccaggatcat gaccagggcc gtcccggaca ggggaacccc
     1981 gggccggttg aagaccagca ggtatccgat gccgaagaag actcccggca cggcggccgg
     2041 caggacgcag agggcatcca gggcccgccc ggcgcggaag gccttctggt ctaccaggta
     2101 ggcggtcagg gtggccagga ccgtggccag gaggcccgcc cagccggcga agaccaggct
     2161 gttccggatg tggtcgagct tggtcacggc cacgcgccac tggtccaggg tgggcgacca
     2221 atccacgccc caggtgcggg tgaaggcgcc ggccacgacg ccgaagtaca ggctcaggat
     2281 caggccggcg agaagcccgc agaagcccag gaggccccac ttcaggaggc cgggcaaggg
     2341 cgggcggtcc agggagcttc cgcgaccacc gatcacggcg taggttcgtc tgcccaccca
     2401 gtagcgctcg gcgaggaaca ggagcaccgc aggcggcagg agcgtggcgg cgaggagtgt
     2461 ggccccgcga aggtcccccc agccgtcgat ccggtaccag gcctcggtgg cgagcagggg
     2521 a
//
LOCUS       DBNGZC010000082         4002 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2354512, whole genome shotgun sequence.
ACCESSION   DBNGZC010000082 DBNGZC010000000
VERSION     DBNGZC010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2354512"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1468)
                     /locus_tag="AC16G0_01485"
     CDS             complement(86..1468)
                     /locus_tag="AC16G0_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR5098322.1"
                     /translation="MGKAFDLEPKDVRLIDTKFRKIVTRIPAPESLGVLQTLYRCEPI
                     ATRGQPPIVWDKAEGCQVYDPYGNMWLDWSSGVLVANAGHGAPEVKEAILEVVEKGLL
                     HTFGFANPQRAELVEYLTRIAPQGLSKVFLLTTGSETTECAIKLSRTHGQKVGGPEKI
                     GMVSFERAFHGRTLGAQMIGGIPSLKTWIVNLDPDMHQVPFPDGFRTPDTRFDLFLRT
                     LVEKGVGPNRVAGVILETFQGGGASFAPKEYIQALARWCRENHVLLIFDEVQASFGRS
                     GKLFSFEHYEVTPDLICCGKGISSSLPLSAVIGRPEVMDLYGPLDMTNTHGGNPVCAA
                     AALASVRKIVEGKLVENARAMGEILHAGLARLVKRHPDIIGAVHGRGLVAGLHIVRRG
                     GKDPDGALAFSIVERAFQKGLLMFAPVGVGEATVKICPPLVISEEAVLDGLAALGEAL
                     EEATGESGRK"
     gene            complement(1793..2227)
                     /locus_tag="AC16G0_01490"
     CDS             complement(1793..2227)
                     /locus_tag="AC16G0_01490"
                     /inference="COORDINATES: protein motif:HMM:NF015862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG/HbpS family heme-binding protein"
                     /protein_id="HIR5098323.1"
                     /translation="MITRQALKLNWEGTNRILQAAVRKAEQGGIPMSIAVVDEATQLL
                     GFARTDEGKPHNIRIALVKARAAASNRLPTGKINSTGQPLNDLMAIALPLAAGTDMYL
                     TFAGGLPIMLDGHCVGGVGVSGGTGEQDTEVAKAGLSALAGK"
     gene            2549..3391
                     /locus_tag="AC16G0_01495"
     CDS             2549..3391
                     /locus_tag="AC16G0_01495"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5098324.1"
                     /translation="MGRLSGKVAMVTGCGGEHGIGRGIARRLAADGADLVLTDVAPRG
                     TPIVNTKPETGWGGLEAVAEEIRAVGHRAITALLDIRSVAQIERVIAATLDAFGRLDI
                     LVNNAAAPPGADRVPVVQLSEEAWDVVLDTNLKGSFLCAKAAATLMLRQKIRGRIINI
                     ASDRAKIGTASLAAYCSSKFGLVGFTQSLAMELAPAGITVNAICPGGVDSERLDYLGR
                     REDGAYDQERRAERVKQLSAAVPLGRLTRPEDVAEVAAFLASDAAEYITGQAINVSGG
                     TIRH"
     gene            complement(3430..>4002)
                     /locus_tag="AC16G0_01500"
     CDS             complement(3430..>4002)
                     /locus_tag="AC16G0_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098325.1"
                     /translation="RLIGEEGSEMSRQVLDRRQSLRRSMQFLVGLGVGAAALNLLGGG
                     SPWLVTAADLPPGATYQATGFYERTAIGRETLVALVLVPLNGTAPPVIQDGPVLERRD
                     LGSATLETLRGQVDGMQQRILFDHGALRAVVYSARTLDGEYAQEKRYLIGRSELEIEN
                     PRARVQKIEGGGGHGGGGSGSGGGSGGGGM"
BASE COUNT          699 a         1359 c         1182 g          762 t
ORIGIN      
        1 cgtcctggtg gtgttcaatc cactggagcg ctcgttcgat ctcaggcatc cggcgtctcc
       61 tgcgattcgc gatgccagca cagcctcatt tgcgcccgga ctctcccgtt gcctcttcca
      121 gggcctcgcc gagagccgcc agtccatcca ggacagcctc ttcgctgatc accagcgggg
      181 gacagatctt caccgtggcc tcgccgacgc ccaccggcgc gaacatgagc agacctttct
      241 ggaaggcccg ctccacgatc gaaaacgcca gggccccgtc ggggtccttg ccgccccggc
      301 ggacgatgtg gagcccggcg accagccccc gtccatgcac agcaccgata atgtccggat
      361 gtcgtttgac cagccgtgcc aggccggcgt gcaggatctc tcccatggcc cgggcgttct
      421 cgaccagctt gccctctacg atcttccgaa cactggccag cgcggccgcg gcgcagaccg
      481 gattcccgcc gtgggtgttg gtcatatcca ggggaccgta gaggtccatg acctccggcc
      541 ggccgatcac cgccgacagg gggagcgagc tggaaatgcc cttcccgcag cagatcaaat
      601 cgggggtgac ctcgtaatgc tcgaaggaga agagcttccc cgatcttccg aaactggcct
      661 ggacctcgtc aaagatcagg aggacatggt tttcccgaca ccagcgcgcc aaggcctgga
      721 tgtattcctt gggggcgaag ctcgccccgc cgccctggaa ggtttccagg atcaccccgg
      781 caacccgatt cggcccgacc cccttctcca ccagtgtccg taagaacaga tcgaaccgcg
      841 tatccggggt tcggaagccg tccgggaacg gcacctgatg catgtcggga tcgaggttca
      901 cgatccatgt cttcaggctg gggatcccgc cgatcatctg ggcccccagc gtccggccgt
      961 ggaaggccct ctcgaagctg accatcccga tcttttccgg accgccgacc ttttggccgt
     1021 gcgtccgaga cagcttgatc gcacactcgg tggtctcgga tcccgtggtc aacagaaaga
     1081 ccttgctgag gccttgcggg gcaatccggg tgagatactc gaccagctcc gcccgctggg
     1141 gattggcaaa gccaaaggtg tgcaggaggc ccttctccac cacctccagg atggcctctt
     1201 tgacttccgg cgctccgtgc ccggcgttcg ccaccagcac gcccgaactc cagtcgagcc
     1261 acatgttccc gtatgggtca tacacctggc agccttcagc cttgtcccag acgatcggcg
     1321 gctgccccct cgtggcgatc ggctcgcatc ggtagagtgt ctgcaggact cccagcgact
     1381 ccggcgcggg aatcctggtg acgatctttc taaatttggt gtcaatcaat cggacgtctt
     1441 tgggctcgag atcaaatgcc ttgcccatgg ggctcgctcc ttcctcggac ctggattcag
     1501 tggcctccca aaaaagcgaa cgccttcccc ggatccggca ggacccaagg aaggcatccg
     1561 atcattctct ggctctcccg gctcaatggc ccacccttcc cggaagatcg tcgctacagc
     1621 ggtcgtcggt gcttcgactg cggcatcgta tcaaatgcgc cgaacagaac tcaagggaat
     1681 tcgctcccgc agggcatccc cccacgctga aggtccgcca tggcacccgg tgttgctgga
     1741 aactgaaccc cgtttgctca ttggtcattg atctcttgtc attgagattt gctcacttcc
     1801 ccgccagcgc ggagagaccg gccttcgcca cctcggtatc ctgctctcct gtccccccac
     1861 tcaccccgac gcctccgacg cagtggccgt ccagcatgat gggtaatccg ccagcgaagg
     1921 tgaggtacat gtctgttccg gccgccaggg gcaaagcgat cgccatgagg tcgttgagcg
     1981 gctgccccgt cgaattgatc tttccggtgg ggagccgatt ggacgccgcc gcccgcgcct
     2041 tgaccagcgc gatgcgaatg ttatgcggct tgccttcgtc cgtccgggcg aagcccagca
     2101 actgtgtcgc ctcgtccacc acggcgatgc tcatggggat tcccccctgc tctgcctttc
     2161 gcaccgcagc ctggagaatg cggttcgtcc cttcccagtt caatttgagc gcctggcggg
     2221 tgatcatggc ccctcccttt ctcgcgcatc cgtttgcgat ttcagccttg aatcaacaag
     2281 atcccagcgg gaattcgagt gaggcgcggt aggtgttcag gatctcctgg atggaagcat
     2341 ctgggaagaa cccgaggcgg tttgctcgtt ccgccgcgat gtatttcgac gggtcatgca
     2401 gatgccccac agccgttccc ctcctggttc cgccacactg taactgccgc tgcggccaaa
     2461 tcctggacag tatcaggtct tcgggtgtac atagtcggtg cgaccctgag gacttcgtgg
     2521 cgaaacaata gcaccgaggg aggtgggcat ggggaggctc agcggcaagg tggcgatggt
     2581 gaccgggtgc ggtggcgagc acggcatcgg acgcgggatc gcccgcagac tagcggccga
     2641 cggggccgac ctggtcctga ccgacgttgc gccacgcgga accccgatcg tcaacaccaa
     2701 accggagacc ggctggggcg ggctggaagc cgttgcggag gagattcgag cggtcggcca
     2761 tcgtgcgata actgcgctac tggatatccg gtcagtcgcc cagattgagc gggtcatcgc
     2821 ggcgacgctg gatgctttcg ggcgcctcga cattctcgtg aataacgccg cagcaccccc
     2881 gggagccgat cgggtgccgg tcgtgcagct ctccgaagag gcctgggacg ttgtcctgga
     2941 tacgaatctc aagggaagtt ttctctgcgc caaggcggcg gccaccctga tgcttcgcca
     3001 gaagattcgc gggcgtatca tcaacatcgc ctcggatcgt gccaagatcg gcaccgcgag
     3061 cttggcagct tattgctcct caaaattcgg cctggtgggc ttcacgcagt ctctcgccat
     3121 ggagcttgca ccggcgggga tcaccgtgaa cgccatctgt ccaggaggag tggacagcga
     3181 aaggctggat tacctggggc ggcgggagga cggagcctac gatcaggaga ggcgcgccga
     3241 gagggtcaag cagctctccg ccgcggttcc cctcggtcgc ctcaccagac ccgaggatgt
     3301 ggccgaggtc gccgccttcc tcgcctcgga tgccgccgag tacatcaccg gccaggccat
     3361 caacgtctct ggcggcacca tcaggcactg accgtacagg caacggactg gattctgggt
     3421 ttttcccggc tacatcccgc ccccgcctga cccgcctcca cttccacttc cgccgccacc
     3481 gtggcccccg cccccctcaa tcttctgcac gcgcgcccga gggttctcga tttccagctc
     3541 gctcctcccg atgaggtatc gcttctcctg ggcatactca ccatcgaggg ttctcgccga
     3601 gtacaccaca gcccgaagcg cgccgtggtc gaacaggatt ctctgctgca tcccgtcaac
     3661 ctgtccccgg agtgtctcca gcgtggctga gccgaggtcc cgcctctcca gaaccgggcc
     3721 gtcctggatc acgggaggcg ccgtcccgtt caatggaacg agcacaagcg ctaccagggt
     3781 ttcccgtccg atggccgtcc tctcatagaa cccggtggcc tgatacgtcg cccccggagg
     3841 aaggtctgcg gcagttacca gccagggaga ccccccaccg agtagattga gagctgcggc
     3901 tccgacaccg aggcctacaa ggaactgcat ggaccttctt aaagactgtc gccggtccaa
     3961 cacctgcctg ctcatctccg aaccctcctc ccctatgagc cg
//
LOCUS       DBNGZC010000083         5322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_593742, whole genome shotgun sequence.
ACCESSION   DBNGZC010000083 DBNGZC010000000
VERSION     DBNGZC010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_593742"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..597)
                     /locus_tag="AC16G0_01505"
     CDS             complement(262..597)
                     /locus_tag="AC16G0_01505"
                     /inference="COORDINATES: protein motif:HMM:NF016839.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIR5098326.1"
                     /translation="MSFAPSPDRPPLRRHLRLVIIFIIALVAVSVMGNRGLIRLYQMH
                     RDKAVLEREIEHLSSANATLAEEVRLLKSDPARIEGIAREELGLVKPGDLVYEFQATP
                     RPPAPPEAR"
     gene            complement(635..1927)
                     /gene="eno"
                     /locus_tag="AC16G0_01510"
     CDS             complement(635..1927)
                     /gene="eno"
                     /locus_tag="AC16G0_01510"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR5098327.1"
                     /translation="MSEISEVRAREILDSRGNPTVEVEVLLESGALGRAAVPSGASTG
                     TREALELRDGDARRYLGKGVSKAVENVNQTIAPEVVGMEATSQTLVDQLLCELDGTAD
                     KSRLGANALLGVSLAVAKAAADEGGLPLYRYLGGVNAKVLPAPMMNILNGGKHADNTV
                     DLQEFMIMPLGASRFSEALRMGAEVFHQLRTVLKKKGYNTAVGDEGGFAPDLKTNEEA
                     VQLILTAIEKAGYKPGTDVFLALDPAASEFYTDGRYVLEGEGGKRLTSEEMVEFYARW
                     VAQYPIVSIEDGMAENDWDGWAALTQRIGGKVQLVGDDLFVTNTKILREGIAKGVANS
                     ILIKVNQIGTLTETLDAILTAHRAGYSAIVSHRSGETEDTTIADLAVATNAGQIKTGS
                     ASRTDRIAKYNQLLRIEEDLGDSAVFWGRESLPRFRRG"
     gene            complement(2003..3433)
                     /locus_tag="AC16G0_01515"
     CDS             complement(2003..3433)
                     /locus_tag="AC16G0_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HIR5098328.1"
                     /translation="MTNIKFGTSGWRGVIAEDFTFTGVRAVSQAIAEYVKGQGPGAPA
                     QGVVVGYDTRFLSEAFAAQAARVLAAEGVRAFLCVRDTPTPVVAFEILRRRAAGGIII
                     TASHNPPEYNGVKFSAAWGGPALPAATKQIEERANAILTGPRMGEVPLPEAEAQAVFE
                     RIDPQSAYLERVRELVDLETIREARLKVVMDPLYGSAQGYLDLLLRDAGCDVSVLHAW
                     RDPTFGGRSPEPSEDHLQELSFQVAETGAHLGLATDGDADRFGLVDADGSFLEPNYFL
                     GLLLYHLVKTRGWTGGVARSVATSHLVDAVARHLQIPVYETPVGFKYIGELIAKDQVV
                     LGGEESAGLSVKGHIPEKDGILACMLAVELVASRGRARLKTLLQELYAEVGTVLTKRL
                     NYHLEPAALEQLRERLQQPPGSLADLAVIEVNRLDGVKMILEDGSWLLLRPSGTEPVV
                     RLYAEAPTEAQLTALVAEGEVLIGNR"
     gene            complement(3467..>5322)
                     /locus_tag="AC16G0_01520"
     CDS             complement(3467..>5322)
                     /locus_tag="AC16G0_01520"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GlgC family sugar phosphate
                     nucleotidyltransferase"
                     /protein_id="HIR5098329.1"
                     /translation="LLEYRLAHQDILAGALNVKLLGRRVEGLDKAIWLGGGSRVDFTA
                     GLRGAVLIGKDTRVEANARITNSVIGNNCVIEEGAVISGSVLWNNVFVGRGAILTENV
                     VGRGCEIKARARLFEGALVADECCIGEGSVVKADVKVWPQKVVEDGATLSTSLVWGER
                     WARSLFGAYGITGLGNIEISPEFAAKLGAAFGATLREHAIVSTSRDSHRTSRIINRAL
                     ICGLLSSGVDVHDLRVAPVPVVRYKIGARGAVAGLHVRKSPYDPELIDIKFFDERGMD
                     ISANREKGIERLFFREDFRRAKVDGTGTLSFPEYDVEYYQQGILSFINQAAIRARAPK
                     VVIDYSYGSASTIFPAILGKLGCEVIALNAYMDEGRITKTAEEFQRSLTQLCEIVRIL
                     GADLGFMLDTGAEKLFLVDDKGDLLPDDLALAVVALLVARTQAVGTIGVPITASGVLE
                     ELVAPHGFRLKRTKIAPRALMEAATLEGAVFVGDAFGGFIFPGFQPAFDAMLASLKIL
                     EMLALSDLSMHEAIRNVPQRATSRDQIPCPWEMKGTVMRSLIEATKDAQVELVDGVKI
                     HLGTDWVILYPDPDRPVFHIQAEASTRAKADQMIGDYRDKLKGWLASVVPA"
BASE COUNT          886 a         1844 c         1676 g          916 t
ORIGIN      
        1 atattgaatt cccaaccaag ccagcatgaa aacagatacg aacatgcgag ccacgaccac
       61 cacatgcgtc tcggttgtca ttgtggcgtg gaacgccaaa gattatgtca tcgagtgcct
      121 tcactccttg cagcacaata cggatcagat ctcggtggag tgctcgaggc aacgtggcag
      181 tagagtatag tgaaggaatc ccggctctgc aaggagtttc aggcggccgc ggaagcggga
      241 aacttcacgc ggcgactgga gttagcgcgc ctcaggggga gccggggggc gtggcgtcgc
      301 ctggaactcg tagaccaggt cgccaggctt taccagtccc agctcctctc tggcgatccc
      361 ctcgatcctg gcggggtcgg atttgagcag gcgcacctcc tccgccaggg tggcgttggc
      421 ggaggagagg tgctcgatct cccgctccag caccgctttg tcgcggtgca tctgatacag
      481 gcggatcagg ccccgattcc ccatcaccga cacggccacc aaagcgatga tgaagatgat
      541 caccagccgc aggtgccggc ggagcggcgg ccgatccggg gaaggcgcga agctcatgct
      601 gccctcagcc tgctcgacaa gacgtccttt tggctcaccc gcgccggaac cggggcagcg
      661 actcccgccc ccaaaaaaca gcgctgtcgc ccagatcctc ctcgatccgg aggagctggt
      721 tgtactttgc aatccggtcc gttcgcgagg cggagccagt cttgatctgg ccggcgttcg
      781 tcgccacggc gaggtcggcg atggtggtgt cttccgtctc cccggagcgg tgggagacga
      841 tggccgagta gccggcccgg tgagcggtca ggatcgcgtc caacgtctcc gtgagcgtgc
      901 cgatctggtt aaccttgatc aggatggagt tggcgacccc cttggcgatg ccctcgcgca
      961 ggatcttggt attcgtgacg aagaggtcgt cccccacgag ttggaccttc ccgccgatcc
     1021 gctgggtcag ggccgcccac ccgtcccagt cgttctcggc catgccgtcc tcgatggaga
     1081 cgatggggta ctgggccacc cagcgggcgt agaactcgac catctcctcg gaggtcaggc
     1141 gcttcccgcc ctccccctcc aggacgtagc gtccgtcggt gtagaactcg ctggcagccg
     1201 gatccagggc cagaaagacg tcggttccgg gcttgtagcc cgccttctcg atggccgtca
     1261 ggatgagctg caccgcctcc tcgttggtct tgaggtcggg cgcgaacccg ccctcatcgc
     1321 ccaccgcggt gttgtacccc tttttcttga ggacggtgcg cagctggtga aagacctccg
     1381 cgcccatgcg gagcgcctcg ctgaaccgcg aggcgcccag cggcatgatc atgaactcct
     1441 gcaggtccac cgtgttgtcg gcgtgcttgc cgccgttcag gatgttcatc atgggtgccg
     1501 gcagaacctt ggcgttcacg ccgcccaggt agcggtagag gggcagaccg ccctcgtccg
     1561 ccgccgcctt ggccacggcc agggagacgc ccaggagggc gttggcgccg agccgtgatt
     1621 tgtccgccgt cccgtccagc tcgcacagga gctgatccac gagggtctgg ctcgtggcct
     1681 ccatccccac cacctcgggc gcgatggtct ggttcacatt ctccaccgcc ttggagacgc
     1741 ccttgcccag gtagcggcgg gcgtcgccgt cgcgaagctc caacgcctcc cgcgtgccgg
     1801 tggacgcccc ggaaggaacg gcggcccggc ccagggcccc gctctccagg agaacctcca
     1861 cctccaccgt gggatttccc cgggaatcca agatctcacg tgcccggact tcgctgatct
     1921 cgctcatgaa tgggctccgt tagtgtgaaa gagaaagcca tcgcgtagaa gatccctctg
     1981 cgggccgacg ccctctcccc gatcatcgat tgccgatcaa gacctccccc tcggcaacca
     2041 gggcagtgag ctgcgcctcg gtcggggcct cggcgtacag gcggactacc ggctccgtcc
     2101 ccgatggccg aagcagcagc caactcccat cctccaggat catcttcacc ccgtccagtc
     2161 ggttgacctc gatcaccgcg aggtccgcca ggctccccgg cggctgttgc agtcgctccc
     2221 ggagctgctc gagggctgcg ggctccaggt gatagttgag tcgtttcgtc aggaccgtcc
     2281 ccacctcggc atacagctcc tgcaggagtg tcttgagccg ggcgcggccc cgcgaggcca
     2341 ccagctcgac cgccagcata caggccagga tgccgtcttt ctccgggatg tgccccttga
     2401 ccgagaggcc cgcgctctcc tccccgccca ggaccacctg gtccttggcg atcagctccc
     2461 cgatgtactt gaatcccacc ggcgtctcgt aaaccgggat ttgcaggtgg cgcgccacgg
     2521 catccacgag atggctggtg gccacggatc gggccacacc ccccgtccag ccacgcgtct
     2581 tcaccagatg atacaggagg agccccagga agtagttggg ctcgaggaag ctaccgtcgg
     2641 cgtccacgag gccgaaccgg tccgcgtcgc cgtccgtggc caggcccaga tgggccccgg
     2701 tttcggccac ctggaaggat aactcctgga ggtggtcctc tgacggttcc ggtgagcgac
     2761 ccccgaaggt gggatcgcgc caggcgtgaa ggacgctcac gtcgcaaccg gcgtctcgga
     2821 gcagcagatc caggtagccc tgggcagagc cgtagagggg atccatcacg accttgagcc
     2881 gcgcctcacg gatcgtctcc aggtccacca gttcgcgcac tcgctccagg taggcgctct
     2941 ggggatcgat gcgctcgaac acagcctggg cctcggcctc cggcagcggt acctccccca
     3001 tccgggggcc ggtcaggata gcgttcgcgc gctcctcgat ctgcttcgtg gccgcgggca
     3061 gggccggccc accccaggcg gcggaaaact tgacgccgtt gtactcgggc gggttgtggc
     3121 tggcggtgat gatgatcccg cctgccgcgc gacgccggag gatctcgaag gcaaccacgg
     3181 gtgtgggcgt gtcccgcacg cacaggaagg cccggacccc ctccgccgcg agcaccctgg
     3241 ccgcctgcgc ggcgaaagct tccgacagga accgggtgtc gtaccccacc accactccct
     3301 gggcgggggc ccccggtccc tggcccttga cgtactccgc gatggcctgg ctgaccgccc
     3361 ggacccctgt gaatgtgaag tcctcggcga tgacgccgcg ccagcccgag gtaccgaatt
     3421 tgatgttggt catgatgctg tgtgcccccg gtttatccgc cccagctcag gccggtacca
     3481 cgctcgccag ccaacccttc agcttgtcgc gatagtcgcc gatcatctga tccgccttgg
     3541 cccgggtgct tgcctccgcc tggatatgga agacgggccg atccgggtcc gggtacagga
     3601 tgacccagtc ggttcccaga tggatcttca ccccgtccac cagctcgacc tgcgcgtcct
     3661 tggtcgcctc gatgaggctg cgcatgactg tccccttcat ctcccagggg caaggaatct
     3721 ggtcacggct ggtggcacgc tggggcacat tccggatggc ctcgtgcatg ctgaggtcgc
     3781 tcagggcgag catctccaga atcttcaaag acgccagcat tgcgtcgaag gccggctgga
     3841 acccggggaa gatgaagccg ccgaacgcgt cccccacgaa gaccgcgccc tccagggtcg
     3901 ccgcctccat caaggctcgg ggggcgatct tcgtccgctt caggcggaag ccatggggag
     3961 ccaccaattc ctccaggacg ccgctggcag tgatcgggac accgatggtg ccgaccgcct
     4021 gggtccgggc caccaagagg gcgaccacgg ccagggccaa gtcatccggg aggaggtccc
     4081 ccttgtcgtc caccaggaac agcttctctg ccccagtgtc cagcatgaag cccaggtccg
     4141 cgcccaggat ccgcacgatc tcgcacaact gggtcaggga ccgctggaac tcctcggccg
     4201 tcttggtgat ccgaccctcg tccatgtagg cgttgagcgc gatgacttcg cagcccagct
     4261 ttcccaggat ggcggggaag atggtggagg cgctgccgta ggagtagtcg atcaccacct
     4321 tgggggcccg ggcccggatg gccgcctgat tgatgaagct cagaatgccc tgctggtagt
     4381 attccacgtc gtactcggga aagctcagcg tcccggtccc gtccaccttg gcccggcgga
     4441 agtcctcgcg gaagaacagc cgctcgatgc ccttttcccg gttggcagag atatccatgc
     4501 cgcgctcgtc gaagaatttg atgtcgatca gctcagggtc gtagggggac ttccggacgt
     4561 gaagcccggc caccgcccca cgcgccccga tcttgtagcg gaccacagga acgggtgcca
     4621 cccggaggtc gtggacgtcc acccccgacg acaggagacc gcagatgagc gcgcggttga
     4681 tgatccggga ggtccgatgg gagtcgcggc tggtgctgac gatggcgtgc tcccgcagcg
     4741 tggcaccgaa ggccgcaccc agcttggcgg cgaactccgg cgagatctcg atattgccca
     4801 ggcccgtgat gccgtaggcg ccgaagaggg atcgggccca gcgctcgccc cagaccaggc
     4861 tggtggacag cgtggcccca tcttccacca ccttctgggg ccagaccttc acgtccgcct
     4921 tcaccaccga gccttccccg atgcagcact cgtccgcgac cagcgccccc tcgaagaggc
     4981 gcgcgcgggc cttgatctcg cagccacggc cgaccacgtt ctcggtcaat atggctccac
     5041 gccccacaaa gacgttgttc cacaggaccg acccggagat gacggccccc tcctcgatga
     5101 cgcaattgtt cccgatcacg ctgttggtga tgcgggcgtt cgcctcgacc cgggtgtcct
     5161 tgccgatcag gaccgcaccc cgcaagcccg ccgtgaaatc cacccggctg ccgcccccca
     5221 gccagatcgc cttgtccaga ccctccaccc tgcggccgag gagcttgacg ttcagcgccc
     5281 ccgccaggat atcctgatgg gcgagtctgt actccagcag at
//
LOCUS       DBNGZC010000084         4461 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_887173, whole genome shotgun sequence.
ACCESSION   DBNGZC010000084 DBNGZC010000000
VERSION     DBNGZC010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_887173"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2076)
                     /locus_tag="AC16G0_01525"
     CDS             complement(<1..2076)
                     /locus_tag="AC16G0_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR5098330.1"
                     /translation="MRRSLKRSLWVLTLVLAVLGSLVSTATSAEIVRATLKNGLRVVI
                     VRDTLAPVVTTQINYLVGSNETPAGFPGMAHALEHMMFRGSRGLSAAQFATISAAMGG
                     HFNADTQQTVTQYFFTVPADDLEVALRIEVLRMRNLLATEQLWGQERGAIEQEVAQDL
                     SNPEYVFYSRLLEAMYQGTPYAHDALGTRESFQQTTGAMLRKFHADWYTPNNAILIVV
                     GDVNPDRTLATVKRLFEVIPSRPLPPRPEVRLQPLKPSNITLDTDQPYGTAVVAYRLP
                     GFDSPDFAAGEVLADVLDSRRGNLYALVPEGKALFAGFNGSALPKAALGYATAGFPHG
                     GDGAALVASMKAIIADYVKNGIPENLVEAAKRHEIADAEIQKNSVAGLAFAWSQALAV
                     EGRTSPDDDIEAIKKVTVADVNRVAKEYLVNDTAVTAVLTPRPSGQPVASQGFGGAES
                     FAPKQTKPVPVPSWAKAAVTPPRLPAPIPMPTVTELPNGLRLIVQPETISRTVSVFGR
                     VKNNPDLQMPEKKEGVAEVLDGLFSYGTTSLNRLAFQEALDEIGANQTAGPSFSLQVL
                     ADGFERGVQLLADNLLAPALPEGAFKVVQEETKSTVAGRLQTPNYLSRRALRMALYPA
                     GDPALRQATPETVGSLTLDDVKAYYAKVFRPDLTTIVVTGHVTSEQAKTVIEKYFGEW
                     KAVGPKPETD"
     gene            2397..3596
                     /locus_tag="AC16G0_01530"
     CDS             2397..3596
                     /locus_tag="AC16G0_01530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5098331.1"
                     /translation="MPPQRDIVIVGAGAVGSSIAYHLAKHGAPAQILERESIAARASG
                     KSWAVFAYPPRFFALEGQPQEQLFSMPVGSVGPWLELFWLGYHRLPDIALEIKETAGV
                     DIGYGELSWVRLALTEEQEEANRTALDLQRERGWHEGYWMDASDLKRLFPDIHPQVRG
                     GAVVPYLQVEPYRYTLGLAQAAEAKGVNIRQGEVVGFRKQGSRVAAARLTTGAEVEAD
                     VFILATGPWSGRSTSWLGKEMPVLINREQCIRMEVPKRLPPYGLTAPNGLTIVPKVSG
                     DVIVGHAGLADLQTDFDVSLTTEECKMMLLNDAVELLPSIGEAKLVEQRGDFEAWSPP
                     PRRIQPVLGRHPEWDNVYIATRFGTLGMMMSLGTGRVMADLILAGGKAPARFQHLLEV
                     LSPAGLG"
     gene            3779..>4461
                     /locus_tag="AC16G0_01535"
     CDS             3779..>4461
                     /locus_tag="AC16G0_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5098332.1"
                     /translation="MTTWNIPALLDEDRQHLLHPLYHPADHAAPHVWVRGQGAVLTDA
                     EGREYIDGLACLWNVNVGHGRKELAEAAATQMATQAYATNYTGSSNVPAIQLARRLTE
                     LAYPNLCATYFASGGAEANESAFKTARFYWKAKGRPQKVKIISRQFGYHGVTLAAMSA
                     TAVAGYAKMFEPQVPNFIHTAAPYGYRLEGMKPGETVGQAASRMLEETILRDGPETVA
                     AVIAEPVEGA"
BASE COUNT          812 a         1380 c         1470 g          799 t
ORIGIN      
        1 gtccgtctcc ggcttgggtc ccacggcctt ccactccccg aaatacttct cgatcaccgt
       61 cttcgcctgt tccgaagtca cgtgtccggt gacgacgatg gtcgtcaggt cggggcggaa
      121 caccttggcg tagtaggcct tgacatcatc cagggtgagc gaccccaccg tctccggcgt
      181 ggcctggcgc aacgctggat cgcctgccgg gtacagggcc atgcggagtg cgcgccggga
      241 caggtagttg ggcgtttgga gccgcccggc cacggtgctc ttggtttcct cctggacgac
      301 cttgaaggcg ccctccggca gggccggagc cagcaggttg tccgccagca gttggacccc
      361 tctttcgaag ccgtccgcca gcacctgcag cgagaagctc ggtcccgccg tctggttcgc
      421 cccgatctcg tcgagcgcct cctggaaggc gagtctgttc agcgaggtgg tgccgtaaga
      481 gaagagaccg tccaggacct cggcgacgcc ttccttcttc tccggcatct ggaggtcggg
      541 gttattcttg acgcgcccga agacgctcac cgtgcgactg atggtctcgg gttgaacgat
      601 gaggcgcaat ccgttcggca gctcggtaac cgtgggcatc gggatcgggg cggggaggcg
      661 cggcggcgtc accgcggcct tggcccacga gggcaccggc acgggcttgg tctgcttcgg
      721 agcaaaagat tccgctcctc caaagccctg ggacgccaca ggctgtccgg aggggcgggg
      781 cgtcaggacc gcggtgacgg cagtgtcgtt gacgagatac tccttggcaa cgcgattcac
      841 atcggccacc gtgaccttct tgattgcctc gatgtcatcg tcgggagacg tgcggccctc
      901 cacggcgagg gcctgggacc aggcgaaggc cagtcccgcc accgagttct tctgaatctc
      961 ggcgtccgcg atctcgtggc gcttggccgc ctccacgagg ttttcgggaa tgccgttctt
     1021 cacgtagtcg gcgatgattg ctttcatgga ggcgaccagg gcggcgccgt ctccgccgtg
     1081 ggggaatcct gcggtggcgt accccagggc tgccttgggc agcgcgctgc cattgaagcc
     1141 ggcgaacagc gccttgccct ccggaaccag ggcgtagagg ttcccccgtc gactatccaa
     1201 tacgtctgcc aggacttctc cggcggcgaa gtccggactg tcgaatcccg gaaggcggta
     1261 ggccacaacg gccgtcccgt agggctgatc cgtgtcgagg gtaatgttgc tgggcttgag
     1321 cggctggagc cggacttcgg gtcgcggcgg caggggccgt gacgggatga cctcgaacag
     1381 ccgcttcacc gtcgccaagg ttcgatcggg attcacgtcg ccgacgacga tcaggatggc
     1441 gttgttcggg gtgtaccagt cggcgtgaaa tttcctgagc atggcccccg tcgtctgctg
     1501 gaaggactcg cgggtgccca gggcgtcgtg ggcgtaaggc gtcccttggt acatggcctc
     1561 gagcaggcgg gagtagaaga cgtattccgg attggacaga tcctgcgcga cctcctgttc
     1621 gatggcccca cgctcctgcc cccagagctg ttcggtggcg agcaggtttc gcatgcggag
     1681 cacctcgatg cgcaacgcca cctccagatc atcggcgggg acggtgaaga agtactgggt
     1741 caccgtctgc tgcgtgtcgg cattgaagtg gccgcccatg gcggcgctga tggtggcgaa
     1801 ctgggcggcg ctgagccctc gactgccgcg gaacatcatg tgctccaggg cgtgagccat
     1861 gcccggaaaa ccggctgggg tttcattgga gccgacgaga tagttaatct gggtcgtcac
     1921 gaccggcgcc agcgtgtcgc gcacaatgac gacccgcagg ccgttcttca acgttgcgcg
     1981 gacaatctcg gcgcttgtcg cggtgctcac caggcttccc aagacagcga gcaccagagt
     2041 caagacccac agactgcgct tcaagctgcg tcgcatgaca cctcccttag ccaagaagct
     2101 ctgtgtgaag ttacgtggtc cattaatcct aacacagcag cgcagaatga aaaggcgccg
     2161 atttgtccac ggggattctc ggccatcaga cacgaggtcc cacagccggc gctggcgcaa
     2221 tgcggatctc accccccatg cgttgcaccg ccagcatccg aatggacctt gaatgtcaag
     2281 accggatttg atagaagggg cacacccaga cgggcgattt cagcgtgcaa agcaccgggg
     2341 gagcctgcgg cagatccccg acgggactcc tgggagtttc cgagaggagg ttaaccatgc
     2401 caccccagag agacatcgtc atcgtcggcg caggggccgt cggtagctcc atcgcgtatc
     2461 acctggccaa gcacggagcc cccgcccaga tcctcgagag ggagtctatt gcagccagag
     2521 cctcggggaa gtcatgggca gtcttcgcgt acccgccgcg ttttttcgcc ttggagggcc
     2581 aacctcagga gcaactattt tccatgccgg tggggagcgt gggcccgtgg ctggagctgt
     2641 tctggttggg gtaccaccgc ttgcccgata ttgccctgga gataaaggaa actgccggcg
     2701 tggatatcgg ctacggggaa ctgtcgtggg ttcggctggc cctgacagag gagcaggaag
     2761 aggcgaatcg gacagccttg gatttgcaga gggagcgggg gtggcacgag ggctactgga
     2821 tggatgccag tgacctgaaa cgcctcttcc ccgatatcca cccacaggtg cgaggaggcg
     2881 cggtcgtccc ctatctccag gtggaaccct atcggtacac ccttggcctg gctcaggccg
     2941 cggaagcaaa gggggtgaac atccgacaag gtgaagtcgt tggattccgg aagcaaggct
     3001 cgagggtcgc cgcagctcgc ttgaccacag gagctgaggt cgaggccgat gttttcatcc
     3061 tggccacagg gccatggagc ggccgcagca cttcctggtt gggaaaggag atgcccgtcc
     3121 tgataaaccg ggagcaatgc attcggatgg aggtgccgaa gcggctcccc ccatatgggc
     3181 tgacggcgcc caatgggctg actatagtcc ccaaggtcag cggcgacgtg atcgtgggcc
     3241 atgcgggatt ggccgatctc cagacggact ttgatgtcag cctgaccaca gaagaatgca
     3301 agatgatgct gttgaacgat gccgttgagc tgctcccatc catcggcgag gccaagctgg
     3361 tcgagcagcg gggggacttc gaggcatggt ccccgcctcc gagacgaatc cagccggtgc
     3421 tgggccgtca tccggagtgg gacaatgtct acatcgccac acgcttcggc acgctgggga
     3481 tgatgatgag cctggggaca ggccgggtca tggccgatct catcctggct ggcggcaagg
     3541 cccctgcccg tttccagcac ctgttggagg ttttgagccc ggccggtctg ggatgaccac
     3601 cagccggccg gaggccgtgt aggccacaca cagatgtcac caaatccacc acggaaagga
     3661 ggagttacct ctgagctagc tcttggcttt ctgcaacccg tggatccttc ggacaagaca
     3721 ccgagaacga gtttgagcag atctcgagaa ctgagacttc tgcgggagga cacgacccat
     3781 gaccacatgg aatatcccgg cgctcctcga cgaagaccgg cagcacctcc tccatcccct
     3841 ctaccaccca gcggatcacg ccgcgcctca tgtctgggtt cgaggccagg gggccgtctt
     3901 gaccgatgcc gagggccggg agtacatcga cggcctcgcc tgcctctgga acgtgaatgt
     3961 cggccacggg cggaaggagc tggccgaggc cgccgcaacg cagatggcga cccaggccta
     4021 tgcgacgaac tataccggct cctccaatgt gccggccatt cagctcgccc gtcgcctcac
     4081 ggagctcgcc tatcccaacc tctgcgccac ctattttgcc agcggcgggg cggaggcgaa
     4141 tgagagcgcc ttcaagacgg cccgcttcta ctggaaggcg aagggccgcc ctcagaaggt
     4201 gaagatcatt tcccgccagt tcggctacca cggggtgaca ctggccgcca tgagcgctac
     4261 tgccgtggcc ggctacgcca agatgttcga gccgcaggtg ccgaacttca tccacacggc
     4321 cgctccctac ggctaccggc ttgaagggat gaaaccgggc gagacggtgg gccaggccgc
     4381 atcccgcatg ctggaagaga ccatcctgag ggatggaccc gagaccgtgg ccgccgtcat
     4441 cgccgagccc gtggagggag c
//
LOCUS       DBNGZC010000085         2823 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_985322, whole genome shotgun sequence.
ACCESSION   DBNGZC010000085 DBNGZC010000000
VERSION     DBNGZC010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_985322"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="AC16G0_01540"
     CDS             <1..572
                     /locus_tag="AC16G0_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HIR5098333.1"
                     /translation="TKLARACGANFRVNVYQPVKSDAFSLGYEEFWEGWRRLLETTGV
                     VSTTEPILSAMLGLDGLAGCACGRRTIRVSPKRRVIPCVYWRGAGLPLERLWELGEAV
                     FQTADFCGARLVPAACRGCALEATCGGGCMGRRLLAGDPTAPDPYCPIIRGDSLGLSA
                     RVLPGTALLKSGSACTTILRAEAGDGREV"
     gene            569..1579
                     /locus_tag="AC16G0_01545"
     CDS             569..1579
                     /locus_tag="AC16G0_01545"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HIR5098334.1"
                     /translation="MSDPRAPRPAVVPDLPAVLYVETTNRCDSLCQTCIRTFNTLEPI
                     KDLTLDELIRIVAQFPRLERVILHGIGEPLLNPDLFQMIAYLKGRDACVLFNTDAIGL
                     TGKKREGLVQSGLDELRVSLDAATPETYRAIRGVAAFGRVCENVAALADLRRKASTGA
                     PVISMWFTVLRRNVGELPDFIRLAGKIGADRVHLQRLVHYGQGLAVEEQSLHGALSAL
                     ENQSMAEASRLAGEMGIQLQASGNTTPAASLTPEEQRRPWSGCQRPWTLSYITANGNV
                     LPCCISPWTAKDYQGLRLGNVFAQQFLDIWNGARYQEFRTRFESDGAPDPCRGCGRLW
                     SI"
     gene            1681..2364
                     /locus_tag="AC16G0_01550"
     CDS             1681..2364
                     /locus_tag="AC16G0_01550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5098335.1"
                     /translation="MTVIIPALDEEGTIGTTVAQLDRTVIHEVIVVDNGSTDRTAECA
                     RTAGARVVLEQRRGYGSACSAGVRAAVASEVLVFLDGDGSDVPEDLPVVLGPILRGEA
                     DLVLGSRTLGEREPGSVSRHQLWGNRLVISLLGRLTGVRLTDFGPFRAIRTEALRRLD
                     MSHPTYGWPIEMVVKAIRRDLRILEVPVRTRRRMIGRSKVAGTVRGSLLAGYHLLVTV
                     VRYAWGPNP"
     gene            2404..>2823
                     /locus_tag="AC16G0_01555"
     CDS             2404..>2823
                     /locus_tag="AC16G0_01555"
                     /inference="COORDINATES: protein motif:HMM:NF021365.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HIR5098336.1"
                     /translation="MRRALFVMAKDPRAGQVKTRLCPPLTPEMAARLYLCFLLDVVDL
                     VAGIPGVDPVIAFSPPDAREEFSRLVSGQFLLIPQQGADLGARLENSFRLLFQQGYDQ
                     VAAVSTDSPDLPLEYLREAFTRLEKAPVVLGPCPDGGY"
BASE COUNT          453 a          901 c          967 g          502 t
ORIGIN      
        1 tgaccaagct ggcgcgggcc tgcggtgcga acttccgggt gaacgtgtac cagccggtca
       61 agtccgacgc cttctccctg ggctacgagg aattctggga gggctggagg cgtcttctgg
      121 agaccaccgg cgtagtcagc acgaccgagc ctatcctgag cgcgatgctc gggctggatg
      181 gtctggcggg atgcgcctgc gggcgtcgga ccatccgcgt gtctccaaag cgtcgggtca
      241 ttccgtgcgt gtactggcgg ggggcgggcc tgccgctgga gcggctctgg gagcttggtg
      301 aggccgtctt ccaaacggcc gacttctgcg gtgcccgcct cgtgcccgct gcctgccgcg
      361 gctgtgctct tgaggccacc tgcggaggcg gttgcatggg tcgccggttg ctggcaggag
      421 accccaccgc cccggacccg tactgcccca tcatccgcgg agacagcctt ggcctgagcg
      481 cacgggtact tcctggaacg gccctgctca aatccggatc ggcctgcacc accatcctgc
      541 gagcggaagc cggtgacggg agggaggtgt gagtgacccc cgagcgccac gacccgctgt
      601 ggttccggat ctgcccgcgg tgttgtatgt cgaaacaacc aaccgctgcg actctctctg
      661 ccagacctgc atccgcacgt tcaataccct cgagccgatc aaggatctga cgctggacga
      721 gctgatccga attgtggcgc agttccctcg tctggagcgg gtcatcctgc acggcatcgg
      781 agaacccctg ctcaaccccg acttgttcca gatgatcgcc tacctcaagg ggcgggacgc
      841 ctgcgtgctt ttcaacaccg acgccatcgg cctgacaggg aagaagcggg agggtctcgt
      901 ccagagcgga cttgacgagc ttcgcgtctc cctggatgct gcaacgcccg agacctaccg
      961 ggccattcgc ggcgtagccg cctttggccg ggtctgtgag aacgtcgcag ccctggcgga
     1021 cttgcgacgg aaggcgagta ccggcgcgcc cgtcatctcc atgtggttca cggtgctccg
     1081 gcgcaacgtg ggcgaactcc ctgatttcat ccgcctggcc gggaagatcg gtgcggatcg
     1141 ggtgcatctc cagcgcctcg tgcattacgg acaagggctg gcggtggagg agcaatcact
     1201 tcatggcgcc ctctctgctc tggagaatca atccatggcc gaggcctcgc gtcttgcggg
     1261 agaaatggga atacagctcc aggcgtcggg caacaccacg ccggcggcga gcctgacccc
     1321 tgaagagcag aggcgcccct ggtccggctg tcagcgcccc tggacgctca gttacatcac
     1381 cgcaaacggc aacgtgctcc catgctgcat atctccatgg acggccaagg actaccaggg
     1441 tctcagactg ggcaatgtgt ttgcccagca gttcctggac atctggaacg gagcacgcta
     1501 tcaggagttc cggacccgct tcgagagcga tggggcgccg gacccctgcc gtgggtgtgg
     1561 tcggctgtgg tcgatttagg gacggtcgca ttcgggcggg gcgcgaccgc gccgcgacga
     1621 aacatgaccc aaccggaagc gacggctctt gggccaccga cggctcacga cgtccggcga
     1681 gtgaccgtga tcattcctgc cctggatgag gaggggacga tcgggaccac agtggctcaa
     1741 ctcgatcgga ccgtgattca cgaggtcatc gtcgtggaca acgggagcac cgatcggacg
     1801 gcggagtgcg ccaggaccgc aggcgcgcgg gtcgtgttgg agcagcgccg cggctacggg
     1861 agcgcgtgct ccgcgggggt tcgggccgcg gtcgcgtcag aggtcctcgt gttcttggac
     1921 ggcgacggga gtgacgtacc cgaggatctc ccggtcgtac tcgggccgat ccttcgcggg
     1981 gaagcggatc tggtgctggg ctcgcgcacg cttggggagc gggagccggg gtcagtgtct
     2041 cgacaccagc tctggggaaa tcggctggtg atcagcctcc tcgggagact caccggggtg
     2101 cgcctcacgg acttcggccc attccgggcc atccgcaccg aggccctgag gcgactagac
     2161 atgtcccatc ccacctacgg ctggcccatc gagatggtgg tgaaggccat ccggcgagac
     2221 ctccgcatcc tggaggtacc cgtgcgcacc cggcgacgga tgatcggtcg gtccaaggtg
     2281 gcgggaactg tccggggaag tctgctggcc ggatatcatt tgctcgtcac ggttgtccgc
     2341 tatgcttggg gacccaatcc atgagcgacc gtacctcctc catcccgtga acgcggagcc
     2401 aagatgcgac gggcgctgtt cgtgatggcc aaggatccca gggcggggca ggtgaagacg
     2461 cgcctgtgtc cgccgctcac gcccgagatg gcggcgcgcc tgtatctgtg cttcctgctc
     2521 gacgtggtgg atctggtggc ggggattccg ggggtggatc cggtgatcgc cttctcgcca
     2581 cctgacgcac gcgaggagtt ttcccgcctc gtatcagggc agttcctgct catcccgcaa
     2641 cagggcgcag acctcggagc gcggctggag aactcgttcc gtctcctgtt ccagcaaggc
     2701 tacgaccagg tcgccgcggt ttcgacggac agtccggacc tgcccctgga atacctgcgg
     2761 gaggcgttca cccggcttga gaaggccccg gtggtcctgg ggccgtgccc cgacggcggc
     2821 tat
//
LOCUS       DBNGZC010000086         6510 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1963563, whole genome shotgun sequence.
ACCESSION   DBNGZC010000086 DBNGZC010000000
VERSION     DBNGZC010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6510)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6510)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6510
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1963563"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="AC16G0_01560"
     CDS             <1..225
                     /locus_tag="AC16G0_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098337.1"
                     /translation="GDPEAIATLKQWRQELEKAEEAEIETGEERPFVVTYSVTKDRTI
                     WAHSLAEAEEMAETDLAYEGDVEILDVEPE"
     gene            226..456
                     /locus_tag="AC16G0_01565"
     CDS             226..456
                     /locus_tag="AC16G0_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098338.1"
                     /translation="MLTRIWREVYDPARQQWVMLPGSERVTTDAPAPLRRGLRDPVGG
                     PRERVLMPYVTKSRPLYTLAKVRRHREGRKRG"
     gene            704..856
                     /locus_tag="AC16G0_01570"
     CDS             704..856
                     /locus_tag="AC16G0_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098339.1"
                     /translation="MQDYMTEAERLVAAFKPGQTVSIPEETRKRFLAAVDQLMVRTAM
                     TPTEPA"
     gene            856..1137
                     /locus_tag="AC16G0_01575"
     CDS             856..1137
                     /locus_tag="AC16G0_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098340.1"
                     /translation="MDLDIQKTVTVQAKELRIYTKVRDEFSASLHDQDGVMICKQDQD
                     YVPGFMPGDHYGDYLILNIDVETGQITNWRRPPVAEVAAWVAKCRGESP"
     gene            1149..1224
                     /locus_tag="AC16G0_01580"
     tRNA            1149..1224
                     /locus_tag="AC16G0_01580"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1182..1184,aa:Thr,seq:cgt)
     gene            1362..1598
                     /locus_tag="AC16G0_01585"
     CDS             1362..1598
                     /locus_tag="AC16G0_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098341.1"
                     /translation="MDVTVVLSALAFCGATVLREGVVGDEQFVRLAGNKYATTWVLVR
                     DLPVIDAECRKYGKPGLYVENIGEPKTREPWRPE"
     gene            1839..2024
                     /locus_tag="AC16G0_01590"
     CDS             1839..2024
                     /locus_tag="AC16G0_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098342.1"
                     /translation="MSHEQVIQQVSLDDLEDFLEYAPRPRPLDTRTVLLRLVREVKKL
                     RAEMDALRRSADLPWRL"
     gene            2157..2393
                     /locus_tag="AC16G0_01595"
     CDS             2157..2393
                     /locus_tag="AC16G0_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098343.1"
                     /translation="MRLALRWGAEFEQLIDGRWRPKDLRSGMWATEALKTIAALLAYA
                     DGHAYNRVCREDPEDVQRRLDALDPNDYPEVLAG"
     gene            2644..3006
                     /locus_tag="AC16G0_01600"
     CDS             2644..3006
                     /locus_tag="AC16G0_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098344.1"
                     /translation="MATQVRHGSNPQFTVKTSGKFRCVIEGYYTDQGVYIRRDPRRSR
                     RFFLWRWFDACRAAVVEEAEPATEGLGAGGVTEVQWHSERPEKRVAELLVLRTCFRSF
                     YEQTMKQLGHDPNGAYRL"
     gene            3615..4025
                     /locus_tag="AC16G0_01605"
     CDS             3615..4025
                     /locus_tag="AC16G0_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098345.1"
                     /translation="MGEAGVGVNRPGVTRTREQEAALQAQMAAHAREQALIAQRNAEP
                     PPPPVRRGFPSRAGSLDKTLLGLGVTVGEVYGQNPETAFTDDDEAEREALLKEQQAQR
                     IAASVLNTPTQPTAGEFTEPDVVRDAEDEADDRR"
     gene            4012..4308
                     /locus_tag="AC16G0_01610"
     CDS             4012..4308
                     /locus_tag="AC16G0_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098346.1"
                     /translation="MIAADLTWTQITALTLAWLLILATAWVGFLMWFRVRALKGRERV
                     RLHESRFGALGVSAPDEADPEHQPMTDAEEALREQIARTQRRVMRQVGNRTEAL"
     gene            4471..4605
                     /locus_tag="AC16G0_01615"
     CDS             4471..4605
                     /locus_tag="AC16G0_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098347.1"
                     /translation="MDQTLALLPGVCDAVGLPRHDVYGRHHTNVFVVWTAAGVTQPAG
                     "
     gene            4634..5209
                     /locus_tag="AC16G0_01620"
     CDS             4634..5209
                     /locus_tag="AC16G0_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098348.1"
                     /translation="MWRRWRTMRWTTKAGVVAALVMILGTSGTLFYAWAQATQFFVSN
                     IAPQVNGTSTVGLDVGLATRKWRHAYFAGTVTANQFSPQLSAPTTLTVGNGTTITKVQ
                     VFSQVVSPTLMAAPAGPAGVFSSAEQTVTVNGLTTADKVFVNGPAQTALCPYSSVRVS
                     GASTLAITFLQLTTGTCQPATGTFEITAIRS"
     gene            5216..6349
                     /locus_tag="AC16G0_01625"
     CDS             5216..6349
                     /locus_tag="AC16G0_01625"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR5098349.1"
                     /translation="MREWPEHGAAISVAALLVGLPWLTWFVWWGYKTGGEAAREIPLF
                     LYTALALMALGGLLTRWDLYLGLFVAYTAGRAVVSHTALALAVAHWVTVGAIVVTWVR
                     AWPASWRTALRWGLVVSGALECGYVAVQAWGDIDPLWSWTGWRVWPAVTVHGTLDHAS
                     LTAAMLAMLTPLAPLWLLPLYAVGLYATHSLLATTAAIIGVLIRWRSRLTARGVAVGA
                     VLSVAAVVWRFWGKSHDSYHERMFAWKTALLTMDHNWGALLFGFGAGAWYRIVPQAQL
                     AAQPGRTEVFYQGHNELLQFFFEGGVIAMALLVAWVYTHRKALFVSGSAVALMVLCTG
                     TFPWHVAPTAVTALAVLALALAALSRREEKPGPAPAQSYRYWM"
BASE COUNT         1097 a         1973 c         2389 g         1051 t
ORIGIN      
        1 ggagatcccg aggctatcgc gacgttgaag cagtggcggc aagaactgga gaaagccgag
       61 gaagcggaaa tcgaaacggg cgaggaacgg ccgttcgtgg tgacgtacag cgtcacgaag
      121 gacaggacca tctgggcgca cagtcttgcc gaagccgagg agatggccga gacagacctc
      181 gcgtatgagg gtgacgtgga gatcctcgac gtggagcccg agtgaatgct gacccggatc
      241 tggcgcgagg tctacgaccc ggcccgtcag cagtgggtga tgctgccggg gagcgagcgg
      301 gtgacgacgg acgccccggc cccgctccgc aggggcctgc gcgatcccgt cggagggcca
      361 agggagcgcg tgctgatgcc ctacgtcacg aagagccggc cactctacac gctggccaag
      421 gtgcggcggc accgggaggg gaggaagcgg ggatgagcaa ggcaagcgag tgggcctcac
      481 gacgccaagc agtcgaatct actcggcccc agcacatgga ggtggtgccg tgtcatggat
      541 cgcctctggg ggcgggcgtc agtgacaagg gcgaggcgac ggtgacatat ggaacacaca
      601 cgatgtggct gacgccagaa gccgccgtca agctcggcca gtggctcctt cacacgtttg
      661 gagaggaacc attttgagag gcgacctcaa gttggagcaa ctagtgcagg actacatgac
      721 cgaggccgag cgtctggtgg cggcgttcaa gccggggcag acggtgagca ttcccgagga
      781 gacgcgcaag cgattcttgg cggcggtgga ccagttgatg gtgcggacgg cgatgacgcc
      841 cacggagccg gcgtgatgga tctggacatt cagaaaaccg tgacagtgca ggccaaggaa
      901 ctgcggatct acaccaaggt ccgtgacgag tttagcgcgt cgctgcacga ccaggacggg
      961 gtgatgatct gcaagcagga ccaggactac gtgcccggct tcatgccagg cgaccattat
     1021 ggcgactacc tgatcctgaa catcgacgtg gagacggggc agatcaccaa ctggagacgg
     1081 ccccctgtcg ccgaggtggc tgcctgggtc gcgaagtgcc ggggggagag cccatgacat
     1141 catcgcgtgc ggcggtagct cagtggcgag agcagcgccc tcgtaacgcg caggtcgcag
     1201 gttcgactcc tgcccgccgc gccatcgtga tggggttgct cgtgctctgc tgctgtcagg
     1261 ggcttggcgg tggcctgtgc tgcgcggagc aggcggcgtg cctggggctg gtgcaggggt
     1321 gcctgtgcgt gccggggtgc agcggggtgc aggggccgta aatggacgtg acggtggtgc
     1381 tctcggcctt ggccttctgc ggcgcgacgg tgctgcggga aggcgtcgtg ggggacgagc
     1441 agttcgtgcg gctcgcgggc aacaagtacg ccacgacgtg ggtgctggtc cgtgaccttc
     1501 ccgtgatcga cgccgagtgt cggaagtacg ggaagccggg gctgtacgtc gagaacatcg
     1561 gcgaaccgaa gacccgtgag ccatggaggc ccgaatgagc cgcgaggggc cgtgattgga
     1621 gatggggctc tacgcctgcg gcccgtgttt cgagcagtac cgactgactc ccgcgtttgg
     1681 gaccgtcgtc ggccaatgcg aaatatgctt cgacttgcat gacgatgact cctcgaagtt
     1741 ctgccagttt acctgggatg tgacacgccg cgtgggggct cccgatgacc agccgattcg
     1801 tgagcaactg catcggttga tggaggtgag gagaacggat gagccacgag caagtgattc
     1861 agcaggtgag cctcgatgac ctcgaagact tcttggaata cgcgccgcga cctcggccgc
     1921 tcgacacccg tactgtgctc ctacgtctcg tgcgggaggt caagaaacta cgggctgaga
     1981 tggacgcctt gcggcggtcg gctgacctcc cgtggcgctt ataggcgatg gagggaagag
     2041 ccatgcctca tcgcgccctc acggccgggg acatccgcgc cctcaagcag ggggtcccga
     2101 agatcctcgc gggccaggaa ctagtctggg gcctcggccg ggaacgctga ccctcgatgc
     2161 ggctggccct ccggtggggg gccgagttcg agcaactcat tgacgggcgg tggcgaccga
     2221 aggatcttcg cagtgggatg tgggccaccg aggcgctgaa gactatcgct gccctgctgg
     2281 cctacgcaga cgggcacgcc tacaaccgcg tgtgccgcga ggaccccgag gacgtgcagc
     2341 ggcggctgga cgcgctcgat ccgaacgact acccggaggt gctggcggga tgaaggcaaa
     2401 gaatcgggag cacaagagac tcctggccct tgtcgtgcga acccctttcg actgggagcc
     2461 gtggggcgac gagtcagtga acggtgatta caccgattgc tcacaggggt gccgatggta
     2521 tcgcccgctc gaaggcgacc tcgcgtggga ctggggggtc tgcacgaacc ccaagagtca
     2581 tcggtgcggg ttgctgacgt ttgagcatca aggatgccga gcgtatgatg gggagcagtc
     2641 ctgatggcaa cccaggttcg ccacggctcc aatccccagt tcaccgtcaa gacctccggc
     2701 aagttccggt gcgtgatcga gggctactac acggaccagg gcgtctacat ccgccgggac
     2761 ccccgacgga gccggcgatt cttcctgtgg cggtggttcg atgcctgtcg agcggccgtg
     2821 gtcgaggagg ccgagccggc gaccgagggg ctgggggccg gcggggtcac cgaggtgcag
     2881 tggcactccg agcggcccga gaagcgggtg gccgagttgc tggtgctgcg gacgtgcttc
     2941 cggtccttct atgagcagac catgaagcag ttgggccacg atccgaacgg ggcgtatcga
     3001 ctctaacccg aggaggcagt cggccgctca actccagcag gccccgcaga ccggcggggc
     3061 atgaggctgg gccgggcaac gtggcaacat cgcgggaggt gccctcgtcg gataggctat
     3121 actcctctcc gacctccggg gggcagcgcg tcccgcgcac gatccctgct cgtgccctcc
     3181 gggggtcagt aggaaggtgc ttgatacccc gagaagggcg gtgcgggggc aggcaggtct
     3241 cagtcgcgct ggactaggtg aagaggctgt ggtggagagc cggcaaaggc gtcaacggcg
     3301 ctgtccggta gccacgccga cgcagatggc cgtgagcgtt ctcatcacgg tgcttgacgc
     3361 gacccggatc tgaagccgga atcccacacg agagcccggc gatggcagca cccggaggca
     3421 ctccgttgct ctgcctccgg ccctttcttt cctcggaacc ccgcccgtag tgttctgccc
     3481 ttgacttccc cccatcaaag gccccacaat cccgccaagc gagggtgtcg tctgcgactg
     3541 acggcctagc ccccctctga tgggtgccag tctctcacgg gtgggagaca cccccgaggg
     3601 aggaggcatc cctgatgggc gaagcgggag tcggggtgaa ccggcctggg gtgacccgga
     3661 cccgagagca ggaggccgcg ttgcaggcgc agatggccgc gcacgctcgc gagcaggctc
     3721 tcatcgcgca acggaacgcc gaaccgcctc cgccgccagt ccgccgggga ttcccgagcc
     3781 gtgctggcag tctggacaag acgctcctcg gcctgggcgt gaccgtcggc gaggtgtacg
     3841 ggcagaatcc cgagacggcc ttcaccgacg acgacgaggc cgagcgcgag gccctcctga
     3901 aagagcaaca ggcccagcgc atcgccgcca gcgtcctcaa caccccgacc cagcccaccg
     3961 ccggagagtt caccgagccc gacgtggtgc gggacgccga ggatgaggcc gatgatcgcc
     4021 gctgacctca cctggacgca gatcaccgcg ctgaccctcg cgtggctcct catcctggcg
     4081 acggcttggg tgggtttcct gatgtggttc agggtgcggg cgctcaaggg acgagagcgc
     4141 gtgcgcttgc acgagtcccg cttcggagcc cttggggtga gcgctcccga cgaggcggac
     4201 cccgagcacc agccgatgac ggacgccgag gaagccttgc gcgagcagat tgcgaggacc
     4261 cagcgccggg tcatgcgaca ggtggggaac cggacggagg cgctgtgaac gacgccgtcg
     4321 agttcccgca tcacccgcac atttgccggg actgcggccg agtgtggcag tgtagtcagc
     4381 acctttgctt ggaatcgggg ttgccgtgcc cccgctgccg ccgccgcgtg atacccgtca
     4441 ggatcggatg agcctggtcc tgcgggagcc gtggaccaaa cactggcatt attgcccggt
     4501 gtgtgcgacg cggtgggatt gccacgacac gacgtgtatg ggcgacatca cacgaacgtg
     4561 ttcgtggtgt ggacggcggc aggagtgacc caaccggccg ggtgagccga atcacccaag
     4621 gagagagcag accatgtgga gacggtggcg gacgatgcgg tggacgacga aggcgggggt
     4681 ggtcgcggcc ctcgtgatga tcctcgggac gagcggcacg ctgttctacg cctgggcgca
     4741 ggccacgcag ttcttcgtgt ccaacatcgc gccgcaggtc aacgggacct ccacggtggg
     4801 gctcgatgtt ggcctggcca cccggaagtg gcggcacgcc tatttcgcgg gcacggtgac
     4861 ggcgaaccag ttttccccgc aactgagcgc tccgaccacg ctcaccgtgg gcaacggcac
     4921 gaccatcacc aaggtacagg tcttcagcca ggtggtgagc ccgaccttga tggccgcccc
     4981 ggctgggcca gcgggcgtgt tctcgtcagc ggagcagacg gtgacggtga acgggttgac
     5041 gacggcggac aaggtctttg tcaatggccc ggcgcagacg gccctgtgcc cgtactccag
     5101 cgtgcgggtg agcggggcgt ccaccttggc catcacgttc ctgcaactga cgacgggcac
     5161 ctgtcagccc gcgaccggca ccttcgagat cacggcgatt cgttcgtagg ggccgatgag
     5221 ggagtggccg gaacacggcg cggccatctc ggtcgcggca ctcctcgtcg gtctgccgtg
     5281 gctgacgtgg ttcgtctggt gggggtacaa aaccggcggc gaggcggcgc gggagatccc
     5341 gctcttcctc tacacggccc tggcgctcat ggcactgggg ggcctgctca cgcggtggga
     5401 cctctacctc ggcctgttcg tggcctacac ggcggggcgg gccgtcgtga gccataccgc
     5461 cctggccctg gccgttgccc attgggtgac ggtgggggcc atcgtggtga cgtgggtgcg
     5521 ggcgtggccg gcttcgtggc gcacggcact gcggtggggc ttggtcgtca gcggggcgct
     5581 ggaatgcggg tacgtcgcgg tgcaggcgtg gggcgacatc gacccgctct ggtcatggac
     5641 cggctggcgc gtctggccgg cggtcaccgt gcatgggacg ctggatcatg cgagcctgac
     5701 ggctgcgatg ctcgccatgc tgacaccgct cgcgccgctc tggctcttgc cgctctacgc
     5761 ggtggggctc tacgcgacgc acagcctgtt ggcgacgacg gccgccatca tcggggtgct
     5821 catccggtgg cggtcgcggt tgacggcgcg aggggtcgcg gtcggcgcgg tcctgagcgt
     5881 tgccgccgtg gtgtggcggt tctggggcaa gagccacgac agctaccacg agcgtatgtt
     5941 cgcctggaag acggcgctgc tgacgatgga ccacaactgg ggggccttgc tgttcggctt
     6001 tggggcgggc gcgtggtatc gcatcgtgcc gcaggcgcaa ctggccgcgc aaccggggcg
     6061 cacggaggtc ttctaccagg ggcacaatga actgctccag ttctttttcg agggaggcgt
     6121 gattgccatg gcgctcttgg tggcctgggt ttacacgcac cgcaaggccc tgttcgtctc
     6181 tgggtcggcg gtggccctga tggtgctctg caccgggacg ttcccgtggc acgtcgcgcc
     6241 cacggcggtg acggcgctgg ccgtcttggc gctggccttg gccgcactct ctcggaggga
     6301 agagaagccg gggccagccc cggcgcagtc gtaccgctac tggatgtaaa agggaaggag
     6361 accacccgat gccgtcaggg atcaacgtcg cggcgattcg gatgcagttg gacacggcct
     6421 tcgccgcctt gaacgaggcg ctggatgtgg ccgcgcaggt ggacgcgctg gaaggccagc
     6481 gggccgtggc gctccgcgag gcgagggccg
//
LOCUS       DBNGZC010000087         4805 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_202742, whole genome shotgun sequence.
ACCESSION   DBNGZC010000087 DBNGZC010000000
VERSION     DBNGZC010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_202742"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="AC16G0_01630"
     CDS             <1..479
                     /locus_tag="AC16G0_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5098350.1"
                     /translation="EGRRVFPGLTVRENLILGASNRPMRWQELEDDMAQMFHIFPDLE
                     RLKHALGWTLSGGQQQMLAIARGLMARPHVLLMDEPSLGLAPILVQEVFRVIKEINAT
                     RSTTVLLVEQNASMALSISHRGYVMETGRIVLEGPAQDLLQDPQVKEAYLGGRKAG"
     gene            669..1355
                     /locus_tag="AC16G0_01635"
     CDS             669..1355
                     /locus_tag="AC16G0_01635"
                     /inference="COORDINATES: protein motif:HMM:NF024938.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HIR5098351.1"
                     /translation="MNPIQDALVHGRPALTEAEAKRFLSGFGIPICRETLAVDAERAA
                     TEAERIGFPVVVKASGTTLLHKTEVGGVALNLRTASEVREASQRLLKISGCQAVLVQE
                     MIRGDRELVCGLTRDAQLGPCVMFGLGGILTETLDDAVFRLAPLTSTDALEMLQEIQS
                     AKILGPFRGQAPADRTTLSRILIALGTIALEHEAIREIDLNPVKIRPDGSPIAVDALI
                     TLKATEPAPR"
     gene            1437..2855
                     /locus_tag="AC16G0_01640"
     CDS             1437..2855
                     /locus_tag="AC16G0_01640"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024994.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043758 - acetate-CoA ligase (ADP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HIR5098352.1"
                     /translation="MEDTAKKSLVPFFEPRSVAIIGASATPGKAGYEAIRNVLANGYP
                     GKIHLVNPKGGEILGLPVTPTISDLPEGVDLAVVILPAEATPKALRACAAKGIRHAAL
                     MAGGFAEVDESGSQIQQELVDIIRETGIRVLGPNISGHTSTPQSYTSAFFPLGKIRRG
                     TVSFIAQTGNFATHTMKYILTGEHFGVCRVIGLGNKVDVDESDALEYLADDPETSAIV
                     IYLESFKRPRRFLDIARAVTHRKPVVMLKSGTTEAGKQAAVAHTAAMASEDRLVDGLL
                     RQAGIVRVWEYSHLILAAKALSMLPLPRGNRVSFLAPSGAMLVAYTDLCVRLGLEVPP
                     VSPETVRRLQEISPPYIRMRNPVDIWASATVRGVEFAYREGMEAVLKDPNIDAVVPVL
                     MLTREIGVPPFDFVLDLAKKYPEKPVLVSFSGAKECIEECKAYLEPRGVPTFPDIEQP
                     FEALSILVRCHTAMIRHVPGQN"
     gene            3092..3550
                     /locus_tag="AC16G0_01645"
     CDS             3092..3550
                     /locus_tag="AC16G0_01645"
                     /inference="COORDINATES: protein motif:HMM:NF013629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR5098353.1"
                     /translation="MHTAELKQTLHDRGLRLTRSRMAVLQVLGATGEHLKVAEVHRRA
                     RQIDDRIGLASVYRTMGLLAEMGLVKNVHVEQRHRRYAQITNGHAHHLVCTGCGLVQE
                     FSDCPADELARTLARRMKFRIEGHCMEFFGRCAKCRQQSRTSSRGRRRKS"
     gene            3547..4458
                     /locus_tag="AC16G0_01650"
     CDS             3547..4458
                     /locus_tag="AC16G0_01650"
                     /inference="COORDINATES: protein motif:HMM:NF013465.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HIR5098354.1"
                     /translation="MTWCRRLAVLSLLFSLPAWFVACESRRPQGQTAGFQKMTVVTTL
                     TVLADLIEQVGGDKVQVKALVPPGGEVHTYQPTPDDIRRVAQARIVFYNGAHLEEWID
                     ETIRSAGRAELRIVVLSQGLPTIVASGDEPNPHLWLDVTNAKAYVEKIRDSLSQMDGA
                     NSAYYADRARTYLTKLDELDDWIRAEVGNIPSARRKLVTFHDAFPYFAKRYGFSLQGV
                     VVASPGKEPSARDLSALVQRIKRERVPAVFVEAQFSPKILEVLAHDAGIKVVSNLYND
                     SLSTGPEANSYIAMMKHDVAQIVEALR"
     gene            4455..>4805
                     /locus_tag="AC16G0_01655"
     CDS             4455..>4805
                     /locus_tag="AC16G0_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5098355.1"
                     /translation="MISNSEIRDSKSKEPGVASGKPWKYCLEVKNLSAGYDRGPAIQG
                     ISFAIRPGQMVGVIGPNGAGKSTLFKAILGLMPRLQGEILLHETDAHHQRAMMGYLPQ
                     LDAIDRTFPVSVEDV"
BASE COUNT          903 a         1539 c         1519 g          844 t
ORIGIN      
        1 ccgagggccg gcgcgtcttc cccggtctga cggtgcgcga gaatctgatc ctgggcgcgt
       61 ccaaccggcc catgcgctgg caggagctgg aagacgacat ggctcagatg ttccacatct
      121 tcccggatct ggagcgcctg aagcacgccc tgggctggac gctctccggc gggcagcagc
      181 agatgctggc catcgcccgc ggcctcatgg cgcggccgca cgttctcctg atggacgagc
      241 cctccctcgg cctcgccccc atcctggtcc aggaggtgtt ccgggtgatc aaggagatca
      301 acgccacgcg gagcaccacc gtcctgctcg tggagcagaa cgccagcatg gccctctcca
      361 tttcgcatcg gggctatgtc atggagacgg gacgcatcgt cctggagggg ccggcccaag
      421 acctgctgca agatccccag gtcaaagagg catacctcgg cgggcgcaag gcggggtgat
      481 ggcgttcccc tgcatacggg gacgctaggc tgcattgccc aggtgcccga tccaagaaac
      541 tagggctttc cttctccgcc ttgttccctc agaatccccc cgcccagtca tgcgctggca
      601 tccggcagcc tgaagtccca ccgagacctt ctcttcaagt gaggtcgcat tcaagagacg
      661 gaggggccat gaatccgatc caggacgccc tcgtccacgg ccgaccggcc ttgactgaag
      721 ctgaggccaa gcgtttcctg tccggatttg gcattccgat ttgtcgcgag accctggcgg
      781 tcgatgccga gagggcggca accgaggccg aaaggatcgg cttcccggtt gtcgtgaagg
      841 catccggaac caccttgctc cacaagaccg aggtgggggg cgtcgccctg aacttgagga
      901 ctgcgtcaga agtacgggag gcgagtcaac gactcctgaa gatctcggga tgccaagccg
      961 tgctggttca agaaatgatc aggggagatc gggagctggt gtgcgggctg acgcgggatg
     1021 cccagctcgg cccgtgcgtg atgttcggcc tgggtgggat cctcaccgag accctggatg
     1081 atgcggtctt tcgccttgcc cctctaacca gcacggatgc gctggaaatg ctgcaggaga
     1141 tccaaagcgc gaagatcctc ggccccttca ggggacaagc gccggcggac agaacaacgc
     1201 tctcgaggat cctgatcgca ctggggacga tcgccctgga gcacgaggcc atccgcgaga
     1261 tcgatctcaa tccggtcaag atccggccag atgggtcgcc gatcgctgtg gatgcgctca
     1321 tcaccctcaa agcgacggag cccgcaccgc gctgaccctc gggccgagga atcggcagga
     1381 ccagcccctt tcgatccctt gccaggattc tcctggacgc tgtggggagg catgccatgg
     1441 aagataccgc gaagaagtcg ctggtgccct tcttcgagcc gcgtagcgtc gccatcatcg
     1501 gggcgtcggc cactcccggc aaggcgggtt acgaggccat acgaaacgtc ctggccaacg
     1561 gatatccggg aaagatccat ctggtgaatc cgaagggtgg cgagatcctg ggtttgcccg
     1621 tcactcccac gatctccgac ctgcccgagg gcgtggactt ggcagtcgtc atcctccctg
     1681 ccgaagccac gcccaaggcc ctgcgggctt gtgccgccaa aggtatccgg cacgcggcgc
     1741 tgatggccgg gggatttgcc gaggtggacg agtccgggtc ccagatccaa caagaattgg
     1801 tggacatcat ccgggagacc gggatccggg tcctggggcc caacatctcg gggcatacct
     1861 ccaccccgca gtcgtacaca tccgccttct ttcccctcgg caagatccgg cggggaaccg
     1921 tgtcgttcat cgcgcagacc ggcaacttcg ccacacacac catgaagtac atcctaaccg
     1981 gtgaacactt cggcgtctgt cgggtgatcg gtctgggcaa caaggtggac gtcgacgaga
     2041 gcgacgcact ggagtatctg gcggacgatc ccgagaccag cgccatcgtg atctacctgg
     2101 aaagcttcaa gcggccgagg cgctttctgg acatcgcgag agcggtcacc caccgaaagc
     2161 ctgtcgtcat gctcaagagc ggcacgacgg aggccggcaa gcaagccgcc gtggctcata
     2221 cggcagccat ggcgtcggag gaccggctgg tggacggcct gctgcgccag gccggcatcg
     2281 ttcgcgtctg ggagtactcc caccttatcc tggcggcgaa ggccctttcc atgctgcccc
     2341 tccccagggg gaatcgggtc agcttcctgg cgccgtccgg ggcgatgctg gtcgcctaca
     2401 cggacctctg cgtgcgcctc ggactcgagg tgccgccggt gagtccggag accgtgcgga
     2461 ggctccagga gatcagcccg ccgtacatcc gcatgcgcaa tccggtggac atctgggcct
     2521 cggccaccgt ccggggggtc gagttcgcct accgcgaggg gatggaggcg gttctcaagg
     2581 acccaaacat cgatgcggtc gttcccgtcc tgatgctgac gcgggagatc ggcgtcccgc
     2641 ccttcgactt cgtccttgat ctcgccaaga agtatccgga gaaaccggtc cttgtcagct
     2701 tcagcggcgc gaaagaatgc atcgaagaat gcaaagccta cctcgagcct cggggcgtac
     2761 ccaccttccc ggacatcgag caacctttcg aggccctgtc aatcctcgta cgctgccaca
     2821 cggcgatgat ccgccacgtg ccgggtcaga attgaagctc ggattctatt gtagcttgct
     2881 gacccgcctt cccctcggat ccatcgctct tccgccggca tccccggacg gcctatgagc
     2941 agatgtgtgg tatggcactg gccgccgcgc tcgcgactgg tctccccacc acggcgctct
     3001 cgccgaatcg agcacgagac cggcacttga cacgcctgga aagggatggt atccagattg
     3061 agaaattttc tcaattgatg gcggttcgac catgcacacg gctgaactca aacaaacact
     3121 gcacgatcgt gggctcaggc ttacccggtc tcggatggca gtcctgcagg tgctaggcgc
     3181 aaccggggag cacctgaagg tggccgaggt ccaccggcgc gcccggcaga tcgatgatcg
     3241 gatcgggctg gccagcgtct accggaccat ggggctcctg gcggagatgg ggctggtgaa
     3301 gaatgtgcac gtggagcagc ggcaccgtcg ctacgctcag atcacgaacg gccacgccca
     3361 ccacctggtc tgcaccggct gcggcctcgt tcaggagttc tcggactgcc ctgcggacga
     3421 gctggcacgc acgctggccc gccggatgaa attccggatc gaggggcact gcatggagtt
     3481 ctttgggcgc tgtgccaagt gccgtcagca atcccgcacc tcctcccgtg gccggagacg
     3541 gaagtcgtga cgtggtgcag gcgcctggct gtcctgtcac ttctgttctc gctgcctgca
     3601 tggttcgtcg cctgcgaatc ccgacgaccc cagggacaga cagcgggctt ccagaagatg
     3661 accgtggtga ccacgcttac ggtgctggcg gatctcatcg agcaagttgg gggagacaag
     3721 gtgcaggtca aggcgctggt cccgccggga ggcgaggtcc acacctacca gccgactccc
     3781 gacgatatca ggcgtgtcgc ccaggcccgg atcgtcttct acaacggcgc ccacctggag
     3841 gagtggatcg acgagaccat ccgcagcgcc ggaagggcgg aactacggat tgtcgttctc
     3901 tctcagggct tgccgaccat cgtggcgtcg ggggacgagc ccaacccgca cctctggttg
     3961 gacgtgacca acgccaaggc gtacgtggag aagattcggg acagcctgag ccagatggac
     4021 ggcgccaact ccgcgtatta cgcagacagg gcgcggacct acctgacaaa gctggacgag
     4081 ctggacgact ggatccgtgc cgaggtgggc aatatccctt cggcccgacg aaagctcgtc
     4141 acgttccacg acgcctttcc atactttgcc aagcgctacg gtttctcgct ccaaggcgtc
     4201 gtggtggcga gcccaggcaa ggaaccgtca gcgcgagatc tctccgcctt ggtccagcgg
     4261 atcaagcggg agcgcgtccc ggccgtcttt gtcgaagcgc aattcagtcc caagatcctc
     4321 gaggtcctcg cccatgacgc tgggatcaag gtggtgtcga atctttacaa cgacagcctc
     4381 tccaccggac ccgaggccaa ctcctacatc gccatgatga agcatgacgt tgcgcaaatc
     4441 gtggaggctc ttcgatgatt tcgaattccg aaattcgaga ttcgaaatcg aaagagccgg
     4501 gggtggcctc cgggaaaccc tggaagtact gcctggaggt gaagaacctc tcggccggat
     4561 acgatcgggg gcccgcgatc caagggatca gcttcgccat ccgtcctggc cagatggtcg
     4621 gcgtgatcgg acccaacggc gccggcaaga gtacgctgtt caaggcgatc ctgggcctga
     4681 tgcctcgact gcagggggag atcctgctgc acgagaccga cgctcatcac cagcgagcca
     4741 tgatggggta cctcccccag cttgacgcca tcgaccgaac cttcccggtc tctgtggaag
     4801 acgtg
//
LOCUS       DBNGZC010000088         4438 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_691317, whole genome shotgun sequence.
ACCESSION   DBNGZC010000088 DBNGZC010000000
VERSION     DBNGZC010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_691317"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..849
                     /locus_tag="AC16G0_01660"
     CDS             229..849
                     /locus_tag="AC16G0_01660"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013505664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HIR5098356.1"
                     /translation="MDRKRVGILIFPEVEVLDFCGPYEVFSVTRLNEERRREEPSPFE
                     VLLVAEHWVPVVTTGSMRVLPDAILDHCPPLDVLVVPGGWGTRREMSNSRLIGWIAER
                     VTRVETLASVCTGALLLGKAGVLDGHRATTHWRSLDLLRQLFPAVAVEEKLRVVDEGR
                     IVTSAGISAGIDLALAMVARYHGETVARATARHMEYPYPEDNLRHD"
     gene            978..1583
                     /locus_tag="AC16G0_01665"
     CDS             978..1583
                     /locus_tag="AC16G0_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017299466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR5098357.1"
                     /translation="MSGDLPGGAAPNPTGTSKQPADTALLLRALQFSAERHRDQRRKD
                     ESASPYINHLIAVASVLANVGGVTDITVLVAAVLHDTLEDTRTSAVEIEARFGEEVRR
                     LVEEVTDDKSLAKVERKRLQVEHAPHLSTRAKLIKLADKICNVVDVTHTPPTSWSMER
                     RQEYLDWTERVVAGCRGSNLALERRYDEVLRQGRLAVAAAR"
     gene            1611..2306
                     /locus_tag="AC16G0_01670"
     CDS             1611..2306
                     /locus_tag="AC16G0_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dph6-related ATP pyrophosphatase"
                     /protein_id="HIR5098358.1"
                     /translation="MQERVIMGWSGGKDSVFALHEILRDDRYTVAGLLTTLTAGYDRI
                     SMHGVRRALLRHQAEALGFSLWEAVIPVKASNQEYEAQMGNALAEVRSRFPDVSSCVF
                     GDLFLEEIRAYRERMLGQVGMRGVFPLWRRDTAVLAREFVRLGYKAVVVCVDTSVLPQ
                     RFAGMEFDEDLLRALPAGVDPCGENGEMHTFVYDGPIFARPIAHSKGEVVLRESRFAY
                     CDLIEDHRPGPPV"
     gene            2746..3729
                     /locus_tag="AC16G0_01675"
     CDS             2746..3729
                     /locus_tag="AC16G0_01675"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR5098359.1"
                     /translation="MSEDRRVLSRLHETMVRIRVFEQHAERLFLDKKIPGFIHLYVGQ
                     EAIAAGVISQLRADDYLTSTHRGHGHMLAKGADSKRMMAELFGKASGYCKGKGGSMHI
                     MDFSLGVLGANGEVAGGIPIAAGTGMAIRLIKKTDQVVVCFFGDGASNRGAFHEGLNW
                     AAIYRLPVVFVVEHNCYASTMPTTESTSVKDIAIRAQGYGLEGMCVDGNEVLHVYQAA
                     RHAIQKARQGDGPTLIEAKTYRLKGHFVGDPQKYRDPQEVEARWRDEPIARFERYLLE
                     QQALTEEERRAVWLRYEAEMDEAVRFAEDSPYPQPSEALTDLFVVDSGYDY"
     gene            3742..>4438
                     /locus_tag="AC16G0_01680"
     CDS             3742..>4438
                     /locus_tag="AC16G0_01680"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014452707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR5098360.1"
                     /translation="MAIEMTFADAICRALKEEMERDPTIFTYGEDIARQGGIFGQYKG
                     LLGSYQDRVIDTPISEEVIYGAAVGAALAGCRPVVEFHFADFLFTGMGAIINQIMKIR
                     YMSGGQRALPIVLRGPDGRARAAAAHHSQSVETLFMHIPGIKVAIPSTPADVYGLLKT
                     ALTGQDPVIIFEHKMLYGLKGNVPDEDLYIPFGKADVKRTGRDVTLVTTGRMVHECLA
                     AAKRLAQENGVSCE"
BASE COUNT          793 a         1331 c         1519 g          795 t
ORIGIN      
        1 catcaccagc cttccggtcc cgtccgtctc gcggtctttg cgttaatctt ctaagatacc
       61 ggcggtcgga gagggacagg cggtaagccg atgaatttgc ggccacacgg gcagcggacg
      121 catccagcct gatggttttc tggattgatg tgagctgctg agtctttacg atctcggcga
      181 acttggtggt gagatttccg ggctcggcag ggaagtggag tgcgaggcat ggaccggaag
      241 cgggtgggca tcctgatctt ccccgaggtg gaggttctgg atttctgcgg gccctacgag
      301 gtcttcagcg tgacacgact gaacgaggag cggcgccggg aagagccatc ccccttcgag
      361 gtgctgctcg tcgccgagca ctgggttccg gtggtgacca caggaagcat gcgggttctt
      421 ccggatgcca tcctggacca ctgtcctccg ctcgatgttc tggtggtgcc gggggggtgg
      481 gggactcgca gagagatgag caattcacgt ctcatcggct ggattgccga gcgagtgaca
      541 cgcgtcgaga cgctcgcctc ggtgtgtacg ggggcgctct tgctgggaaa ggcgggggtg
      601 ctggacggac accgggcgac gacccattgg cggtctttgg acctcctgcg ccaattgttt
      661 cccgcagtgg ctgtggaaga gaagctccgg gtggtggacg agggacggat cgtgacgtcg
      721 gcagggatct cggccggcat cgacttggcg ctcgccatgg tcgcgcgtta tcacggcgag
      781 actgtggcgc gagcaacggc ccgacacatg gaatacccct atccggaaga caacctcagg
      841 cacgactgag ggcgaaacgt ccgcgcgaga tgccgcactt cgtgcgggat tgagaacagc
      901 ccgaggcaac tgatgccagg ggatgtattc cacgcgctgg ccgccaagac cgatcggctg
      961 tattcggagt cctccgcatg agcggtgacc tgcccggagg ggctgccccg aacccaactg
     1021 ggacatcgaa acagccggca gacacggccc tgctgttgcg ggcgctccag ttttccgccg
     1081 aaaggcaccg ggaccagcgg cgcaaagatg agagcgcctc cccctacatc aatcatctca
     1141 tcgcggtcgc gagcgtgctg gccaacgtgg gcggtgtcac ggatatcacc gtgctcgtcg
     1201 cagccgttct gcacgacacg ctcgaggaca cgcggacgag cgcagtcgag atcgaggccc
     1261 gcttcggcga ggaggtcagg cgtctggtcg aggaagtgac cgacgacaag agtctggcca
     1321 aggtggagcg gaagcgcctg caggtcgaac acgccccgca cctgtccacc cgggcgaaat
     1381 tgatcaagct cgccgacaag atctgcaatg tcgtcgacgt gacgcacaca ccgcccacca
     1441 gttggtcgat ggagcgtcgg caggagtacc tcgattggac ggagcgtgtt gtcgccggat
     1501 gccgcggaag caacctggcg ctggagcggc ggtacgatga agtcctgcgg cagggcagac
     1561 tggcagtcgc cgcggcacgg tagtctgtcc tggtcgagga gggggaggaa atgcaggaac
     1621 gcgtgatcat ggggtggagc ggagggaagg acagcgtctt cgcgctgcat gagatcctgc
     1681 gcgacgatcg gtacacggtg gccggcctgc tcaccacgct gaccgcggga tatgaccgca
     1741 tcagcatgca cggggtgcgg cgtgccctgc ttcgccatca ggccgaggcc ctgggcttct
     1801 cactctggga ggccgtgatt ccggtcaagg cgtcgaacca ggagtacgag gcacagatgg
     1861 ggaacgcgct ggccgaggta cgctccagat tccccgatgt ctcctcctgc gtcttcggcg
     1921 acctcttcct cgaggaaatc cgggcctacc gcgagaggat gctgggtcag gtcgggatgc
     1981 gcggggtatt tcccctctgg cgacgggaca cggcggtcct ggcccgcgag tttgtccgac
     2041 tcggctacaa ggcggtcgtc gtctgcgtgg acaccagcgt ccttccccag cgattcgcgg
     2101 ggatggagtt cgacgaggac ctcttgcgcg ctctcccggc gggggtggat ccgtgcgggg
     2161 aaaacggcga gatgcacacc ttcgtgtacg acgggccgat ctttgccagg ccgatagctc
     2221 acagcaaggg ggaggtcgtg ctgcgcgaat cacgcttcgc ctattgcgac ctcatcgaag
     2281 atcaccggcc ggggcccccc gtctgacctg acggcttcag cctgttctgc ctgcctcgga
     2341 tctccacgcc gccctctcct ttcgcccgga tccgcgtgtg gtcaggtcaa cgacacaagg
     2401 cggcgagggg tccttgacac cgcggggagc ctctgatatc aagcccgagg gtggcacgga
     2461 gcgatgcgtg gttcgagggg ggctgcatgt ctgccggatg ggcaggctcc agcagtgcgt
     2521 cggcgacttg tccgttcatc gggtgagtcg ccgtcacttt ttgccgtttc ctttggtcca
     2581 ggacgtgggt gggtgacgca ggcgaacgca tccgcctctc tgttcctcca cgagtcgcgc
     2641 ccgtgacccc gcatccgatt cagccatgac agccgccgct cccgcggttc gccctccgga
     2701 ctcctgacga accggcttcc tggtttcatg aatgaggtac ctccgatgag cgaggacaga
     2761 cgggtcctgt cgagacttca cgagacgatg gtgcgtatcc gggtgttcga gcaacacgcc
     2821 gaacggctgt tcctggacaa gaagatcccc ggattcatcc atctctacgt cgggcaggag
     2881 gccatcgcgg ccggggtgat ctcccagttg cgcgccgacg attacctcac cagcacccac
     2941 cggggccacg gccacatgct cgccaagggg gccgacagca agcggatgat ggcggaactc
     3001 ttcgggaagg caagcggcta ctgcaagggg aaaggcggct cgatgcatat catggacttc
     3061 tccctcggcg tcctcggggc gaacggggag gtcgcgggag gaatcccgat cgccgcgggc
     3121 acgggaatgg ctatccggtt gatcaagaag accgaccagg tcgtcgtctg cttcttcggg
     3181 gatggggcct ccaaccgcgg ggcgtttcac gaggggctga actgggcggc aatctatcgt
     3241 cttccggttg tcttcgtggt cgagcacaac tgctatgcct ccaccatgcc gaccacggaa
     3301 tcgacctccg tgaaggacat cgcgatccgg gcgcagggct acggactgga ggggatgtgc
     3361 gttgacggga atgaggtcct gcatgtctac caggccgcgc gccacgcgat ccagaaggcg
     3421 cgtcagggcg atgggcctac tctcatcgag gccaagacgt accgtctcaa ggggcacttc
     3481 gtcggagacc cgcaaaagta ccgggacccc caggaggtcg aggcgcgctg gcgggacgag
     3541 cccatcgcgc ggttcgaacg gtatctcctg gagcagcagg cgctcaccga ggaggagcga
     3601 cgagcggtct ggctgcggta cgaggccgaa atggacgagg cggtccggtt tgccgaggac
     3661 agcccctatc cccagccatc cgaggccctg acggatcttt tcgtggttga ttcgggctac
     3721 gattattgat ggagtgccgc catggccatc gagatgacct ttgccgacgc catctgccgg
     3781 gccctgaagg aggagatgga acgcgacccg acgatcttca cctacggtga ggacatcgcc
     3841 aggcagggcg ggatcttcgg gcagtacaag gggctgctcg gctcctatca ggaccgcgtc
     3901 atcgacacgc cgatctcgga agaggtgatc tacggggccg ccgtcggcgc ggcgctggcg
     3961 ggctgccgcc ccgtggtgga gtttcacttc gcagacttcc tctttaccgg gatgggcgcc
     4021 atcatcaatc agatcatgaa gatccggtac atgtcgggtg ggcagagggc cctccccatc
     4081 gtcctgcgcg gccctgatgg ccgggcgagg gcggcggccg cgcaccattc ccagagcgtc
     4141 gagacgctct tcatgcacat tccggggatc aaggtggcca ttccctcgac gcctgccgac
     4201 gtgtacggcc tcttgaagac tgccctgacg ggtcaagatc ccgtcatcat tttcgaacac
     4261 aagatgctct acggcttgaa ggggaacgta cccgacgagg atctgtacat tcccttcggc
     4321 aaggcagacg tcaagcggac cgggcgggat gtgaccctgg tgaccacggg gcggatggtg
     4381 catgagtgtc tggctgctgc caagaggctc gcgcaggaaa acggggtctc ctgtgaag
//
LOCUS       DBNGZC010000089         1970 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_496203, whole genome shotgun sequence.
ACCESSION   DBNGZC010000089 DBNGZC010000000
VERSION     DBNGZC010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1970)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_496203"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="AC16G0_01685"
     CDS             <1..990
                     /locus_tag="AC16G0_01685"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF040249.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR5098361.1"
                     /translation="VALYTRGFHPSAICGAFGAAAAAGHLFRLSPAKYAIALGLALQQ
                     ASGLLAWASDHTENSRPFNPGLAARNGTTAAYLAHLGFGGPPTPFEGKYDVFTAFSGE
                     RHPEALLADWGKHYYLPEFAYKLYSSCSFTHPGLDALLSLARDHHLQSTDVREIVLRF
                     PRSGAHMIDNNELKSHCAQYILPVGLVFGQVMIDDILQDRMRHPEVARLRAHTRMVAD
                     PKLDRHYPEVYASIIEVATTAGRHLSCRVDYAKGTMQNPLTPEEIHRKYLKLATTVTT
                     AAHAERIADAVQRIDRLRDLVGLCAMLRILRGATPMRTKGETRRPKATGKART"
     gene            1073..1954
                     /locus_tag="AC16G0_01690"
     CDS             1073..1954
                     /locus_tag="AC16G0_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR5098362.1"
                     /translation="MGAVGLGALLAPAVTGTRTASAAAAPQETTIDRIKRTGVFNLGA
                     RDADPPYGFLENNKHVGFSTEIAERVHERVEKELKASIKLNYVSVTSRTRIPLLLNGT
                     IDMEAGATVITKDRVKVVDFSVPFFLTASNLIVPADSPIKRLADLSGKRLGGPRGGLQ
                     EMLFTKKLQAQGIFRSPVRFIGFENPSEGFTALQGGSVDAYTNDGPLVYGLLKSAQDP
                     SKWRAFDPNVDASPQAFPIRQYSSSFATLINLTIVDLCESGRWQELYQKYFVPVGLPK
                     EPDDTLKFLVRLNSWSD"
BASE COUNT          380 a          656 c          600 g          334 t
ORIGIN      
        1 gtggcgctgt acacccgggg ctttcaccct agcgccatct gtggcgcctt cggggccgcg
       61 gcagccgcgg gtcatctgtt ccggctctcc ccggccaaat acgccatcgc cctgggcctg
      121 gccctgcaac aggcctcggg tctcctggcc tgggcgtccg atcacaccga gaactcgcgc
      181 cccttcaatc cgggcctggc cgcgcgcaac ggcacgacgg cggcctatct ggcccacctg
      241 ggcttcggcg gcccgcccac gcccttcgag gggaagtacg acgtcttcac cgccttctcc
      301 ggcgagaggc atcccgaggc cctgctggcg gattggggga agcactacta cctgcccgaa
      361 tttgcctaca agctctattc ttcttgctcc ttcacccacc ctggtctgga tgccttgctc
      421 agcctggccc gcgaccatca cctgcaatcc accgacgtcc gcgagatcgt cctgcgtttc
      481 cccagatccg gcgcccacat gatcgacaac aacgagctga agtcccattg cgcgcagtac
      541 atcctgcccg tcgggctggt cttcggccag gtcatgatcg acgatatcct gcaggaccgg
      601 atgcgccacc ccgaggtcgc ccgcctgcgt gcgcataccc gaatggttgc cgaccccaag
      661 ctggacaggc actatcccga ggtctacgcc tcaatcatcg aggtcgcgac gaccgccggc
      721 cggcacctca gttgtcgcgt agactacgcc aaggggacga tgcagaaccc cctcaccccc
      781 gaggagatcc atcggaagta cctgaagctg gccacgaccg tgacgaccgc cgcccacgcc
      841 gagcggattg ccgatgccgt gcagaggatc gatcggctgc gagatctcgt cggcctttgc
      901 gccatgctgc ggatcctgag gggggccacc ccgatgcgaa cgaaaggtga aacacggagg
      961 ccgaaggcga cgggcaaagc tcgcacgtga actgaaccat ccgcgggagg gaaccattgg
     1021 acgcaagaag attggggatg agttccggac gagtcggcga cggatgctcg gggtgggggc
     1081 agtcggactt ggcgcgctgc tcgcaccggc cgtgaccggc accaggacag cgagcgcggc
     1141 cgcagccccg caagagacca ccatcgaccg catcaagcgc accggagtgt tcaacctcgg
     1201 cgcgcgggac gcggaccctc cctacggatt cctcgagaac aacaaacatg tcggattctc
     1261 cacggagatt gccgagcggg tacacgaacg ggttgaaaaa gagctcaagg ccagcataaa
     1321 actcaactac gtgtcggtga ccagccggac caggatccct ctgcttctca acggcacgat
     1381 cgacatggaa gccggggcga cggtcatcac gaaggaccga gtgaaggtgg tggatttctc
     1441 tgttccgttc ttcttgacag cgtccaatct gatcgtgcct gccgacagtc cgatcaaacg
     1501 gctggctgac ctgtcgggga aacggctcgg gggaccgcgc gggggccttc aggagatgct
     1561 gttcaccaag aagctgcagg cgcaaggcat cttccgcagc ccggtccgat ttatcggatt
     1621 cgagaacccg tcagaaggct tcacggcgct gcaggggggc tccgtggacg cctacacgaa
     1681 cgatgggccg ctggtgtacg gattgctgaa gagcgcacag gatccgtcaa aatggcgagc
     1741 gttcgacccg aacgtggacg cctcgcccca ggcctttccg atccgccagt acagctcgag
     1801 tttcgccacg cttatcaact tgaccatcgt ggacctctgc gagagcggcc gctggcagga
     1861 attgtaccag aagtactttg tgccagtcgg tctcccgaaa gagccggatg acaccctgaa
     1921 attcctcgtc cgattgaact cctggtcgga ctaggctggc gtcgtccgca
//
LOCUS       DBNGZC010000090         3502 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1866362, whole genome shotgun sequence.
ACCESSION   DBNGZC010000090 DBNGZC010000000
VERSION     DBNGZC010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1866362"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="AC16G0_01695"
     CDS             complement(<1..564)
                     /locus_tag="AC16G0_01695"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR5098363.1"
                     /translation="MSSHEVSVVGAGPNGLAAAITLARAGLAVSVYEAKDSIGGGARS
                     AELTLPGIVHDVCSAVHPLALASPFFRTVPLANHGMAWIHPPAALAHPLDDGTAVILE
                     RSIDATARALGSDAAAYRRLMGSLADEWDALGASILGPPLRLPRHPLAIARFGAYALL
                     PARWLAERLFRQALARALFAGIAAHSLL"
     gene            complement(619..2328)
                     /gene="ggt"
                     /locus_tag="AC16G0_01700"
     CDS             complement(619..2328)
                     /gene="ggt"
                     /locus_tag="AC16G0_01700"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione gamma-glutamate
                     hydrolase [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR5098364.1"
                     /translation="MIRSTVVATKDTPLAPRGLVVAAHPLGADVGASILKRGGNAVDA
                     AVATAFAMTVVEPFMSSIAGGGTMLVHLAKRGETIALDFNVQAPAACHETIYPLADGV
                     ATDLFPWRKVADDANVFGHRSVAVPGSVAGLCLALERYGSMELRDILAPAISLARDGF
                     PSDWYLALTTAIHAEELAAFPATARTYLRGGHYIHRPPTIAEGDRVTFPDLARSLDLV
                     GREGSAAFYKGAIAQAIDEEMRAHGGFLTRNDLATYAVRVQPALVGQYRGLGLAFSPG
                     ATGGITSLETLNILSAFPSREVGFDTPQGLHLRAEAVRHAFQDRLKYLGDPAQVEAPW
                     AALASREYGVQVAGAILTHGRRSQRPAPDPWHFEGKTGRAEGPACGKGESGDCTTHIG
                     VVDRQRNMVSLTHTAVSIFASRVVVPGTGILLSNGMLWFDPEPGRPNSVGPGKRALVN
                     MVPVLAFRRGQPYLTLGAPGGRKIISAIPQVLSNMADLKLSPQAAIEAPRLHTEGDDL
                     WVDDRVGEKLPAALRKLGHRVTVRQESPSTLYFSRPVAIRVTKKGLEAGLDHLRAAAA
                     SGF"
     gene            complement(2447..2860)
                     /locus_tag="AC16G0_01705"
     CDS             complement(2447..2860)
                     /locus_tag="AC16G0_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098365.1"
                     /translation="MAETIRLIDYFYIEAPDKPGEGVKVLKQLKEAGVNLEAFSGFPK
                     GRRAQLDFIPSDAAAFKAVAKKAKWKVVGPKKGFLIQGEDRVGAMADLLESLAAAKIN
                     VTASDAICAGAGRFGSLLWVKARDVKRAAKIFGIA"
BASE COUNT          599 a         1122 c         1211 g          570 t
ORIGIN      
        1 gaggagagag tgtgctgcga tacctgcaaa gagcgcacgg gccagtgctt gcctgaacaa
       61 tcgctcggca agccagcgcg cgggcaggag ggcgtacgca ccgaatcgtg cgattgcgag
      121 ggggtggcgc ggcagccgga gcggggggcc gaggatcgag gccccgagtg cgtcccactc
      181 gtccgccaac gaccccatga ggcggcggta ggcggccgcg tccgacccga gcgcgcgggc
      241 ggttgcgtca atggagcgct ccaggatgac ggcggtgccg tcgtcgagcg gatgggccaa
      301 ggctgcgggc ggatgaatcc acgccattcc atgattggcc agcgggaccg tgcggaagaa
      361 cggcgaggcc agggcgagtg ggtgcacggc ggagcagaca tcatggacga tgcccggtaa
      421 ggtcaattcg gccgaccggg cgcctccccc gatgctatcc ttcgcctcat acacgctgac
      481 ggcgagcccg gcgcgcgcca gggtgatggc tgccgccagc ccgttcggcc ccgcgccgac
      541 gacgctcacc tcatgagagc tcatcccggt ttcatgcctg cggtctccgc acggccgtgc
      601 ggtcttctgt tcgggggatc agaagccgga cgcggccgca gccctgaggt ggtcgaggcc
      661 tgcctccaaa cccttcttcg tcacgcggat ggccacgggc cgggagaagt agagcgtcga
      721 cggactctcc tgcctcactg tcacccgatg ccccagcttg cggagggcgg ccggcagctt
      781 ttctccgacc cggtcgtcca cccagaggtc gtcgccctcc gtgtggagcc gcggggcctc
      841 gatggcggcc tgtgggctga gcttcaggtc agccatgttg gacaggactt gagggatggc
      901 ggagatgatc ttgcgcccgc cgggggcgcc aagcgtgaga tacggctggc cccttctgaa
      961 ggcgagcacc gggaccatgt tcaccagggc ccgcttgcca ggccccaccg agttgggccg
     1021 tcccggctcc gggtcgaacc agagcatccc gttcgagagg agaatgccgg ttccggggac
     1081 gaccacgcgg gaggcaaaga ttgagacggc cgtgtgggtg agcgacacca tgttccgctg
     1141 ccggtccacc acaccgatgt gcgtcgtgca gtcgccggac tccccctttc cgcacgcggg
     1201 cccctccgcc cggccggttt tcccctcgaa gtgccagggg tcgggtgccg gcctctgcga
     1261 gcgccggcca tgggtcagga tggcgcccgc gacctggact ccatactccc tcgaagccaa
     1321 ggcagcccag ggagcctcaa cctgagcggg atcgccgagg tatttcaggc gatcctggaa
     1381 ggcgtggcgc acggcctccg cacggaggtg gagaccctgc ggggtatcga aacccacctc
     1441 cctggatgga aaggcggaga gaatgttcag agtctccagc gacgtgatac ctccggtcgc
     1501 gccgggagaa aaggcaagcc ccagtcctcg atactggccg accagggcgg gctggacccg
     1561 aaccgcgtag gtggccaggt cattccgcgt gaggaatccg ccgtgggccc gcatctcctc
     1621 gtcgatggcc tgggcgatgg ctcccttgta gaatgcagcc gatccttccc gcccgaccag
     1681 gtcgaggctg cgggcgagat cgggaaaggt gacccggtcc ccctcggcga tggtcggggg
     1741 tcggtggatg tagtgtccgc ctcgcaggta cgtccgggcc gttgccggga aggcggccag
     1801 ctcctccgcg tggatcgccg ttgtgagggc aaggtaccag tcggaaggga acccgtcgcg
     1861 ggccaggctg attgccgggg cgaggatgtc acgaagctcc atggatccgt agcgctccag
     1921 ggcgaggcag agcccagcca ccgagcccgg aacggccacg gagcgatggc cgaagacgtt
     1981 ggcgtcatcc gccactttcc gccaagggaa gaggtctgtg gccaccccgt cggccagggg
     2041 gtagatggtc tcatggcagg cggcgggggc ttggacgttg aagtccaggg cgatcgtctc
     2101 cccacgcttg gccaggtgga cgagcatggt ccccccgcct gcgatcgagg acatgaatgg
     2161 ctccacgacc gtcatggcga aggccgtcgc cacggcggcg tcgacggcgt tgcccccacg
     2221 cttgaggatg gatgcgccca cgtccgcccc aaggggatgt gcagccacca ccaggccgcg
     2281 tggggccagg ggggtgtctt tcgtcgccac gaccgttgac cgaatcatcc tctccatttc
     2341 cctccagaaa gaaccgccac catggaggcg acttgtctcc cggtgtcagc cggcgcaagt
     2401 cacctcgaca tggtggcggc gaagcgctgt ccgatcagcc cagacgtcag gcgataccga
     2461 agatcttggc ggcgcgcttg acatcgcgcg ccttcaccca gaggagcgag ccgaagcgac
     2521 cggcaccggc gcagatggca tcgctggccg tgacgttgat tttggcggct gccaggcttt
     2581 ccaagagatc cgccatggcc ccgacacggt cctcaccctg gatgaggaag cccttcttgg
     2641 ggcccacgac cttccacttt gccttcttgg caacggcctt gaatgccgcc gcgtcactgg
     2701 gaatgaagtc cagttgggcg cgtcggccct tcgggaaccc ggagaacgcc tccagattga
     2761 ctcctgcctc cttgagctgt ttcaggacct tgacaccctc tcccggcttg tccggcgctt
     2821 cgatgtagaa gtagtctatg agtcggatcg tctcggccat gggaccctcc tttcgtgtgt
     2881 atgcggcttt tcctgtccaa aggaagccgc gggaatcatg gaggtggtgc aaacgggccg
     2941 attgcccccc ttggcgtcga agacctgaat ttccccgtag cgcatgacag aaaccgtgac
     3001 gtatggccag tgctcatacg cattgtatat ctagaggcca cgtataacag gggaccggca
     3061 gggtgtcaat gggtaggcca cactggaagc tgaaattccg aggccttaat gaaggcggag
     3121 aggcgggttt ccctttcgag ggactaccgg gggggtgcga ccaacgcgat gctgctgcct
     3181 ggaatgtgcg acggcgcgcc gccgcagatc cctcacagcg tcgcggggct caaggggctg
     3241 tatctgagct gtcacctgga gggggcaaaa gggcgcccat cgtcccgcac cccgaccggg
     3301 ggtgggataa cccgaagtgc tgggcgggac ggcgtcccgg tcgtcaggga agagtcaggg
     3361 tgtagccgac ggtgcaaaac gccacggcat caccgagctc ctgggccaat cgggcgccgg
     3421 cctgaaggcc ggtgcagtga gacagaccca gcctcttcac gcccagcccc ttcaggatgc
     3481 cgatcgtttg acggagctgc tc
//
LOCUS       DBNGZC010000091        11123 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_691606, whole genome shotgun sequence.
ACCESSION   DBNGZC010000091 DBNGZC010000000
VERSION     DBNGZC010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 11123)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11123)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11123
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_691606"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="AC16G0_01710"
     CDS             complement(<1..611)
                     /locus_tag="AC16G0_01710"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF025065.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR5098366.1"
                     /translation="MAERLPMIFAAPGTYVQAPGILKTASPHLARLGGRIFGVVDPNV
                     WADVEPALAEACRAEGLAWRAERFPGECTEATVATLAEAAKAWAPAAIVAAGGGKSLD
                     TGTLMAESLRLPSAMVPTIASTDAPTSRVAVLYDDNHVQVRVARLRANPALVLVDTAV
                     IVKAPVRFLVAGMGDAISTWPEARANVAGAARTPVGGSQTRAAL"
     gene            complement(672..1928)
                     /locus_tag="AC16G0_01715"
     CDS             complement(672..1928)
                     /locus_tag="AC16G0_01715"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR5098367.1"
                     /translation="MDGKYLTEFLRAQRGEAEGSKAPLKDIRIIDFGSVVAAPFGATT
                     LADYGAEVIKIEPPSVPDAIRYWAMVGEHQPFWLLAGRNKLPVTLNLKHPKGQQVFAR
                     LVERSDVLLENMRPGTLDRLGFPATKLWEINKGLIIGRVSGYGQTGPSAAKPGFGTLA
                     EAMSGFTYMNAHPGGPPTNSPLPLADMIAGLHLALGVILALRHSKRGEFGGQELDLSL
                     YEPLFSLMGADFLTYVLSGTSPTPMGNEAHYTSPRNSYQTKDGKWVVLSASSQATFER
                     LMDLVGRPDLKQEPGFRNNQERTQQASRQLLNHVIGSWIKGRPIQTVLEECEKAGVTI
                     GPIYSMEDIAKDPNIKARGSIISMFDPVSEGDIPFPEAPIRMSGTPGKIRFPGLPMGA
                     ANEVVLGDLLGYSPDAIAALKSSGAI"
     gene            complement(1938..2267)
                     /locus_tag="AC16G0_01720"
     CDS             complement(1938..2267)
                     /locus_tag="AC16G0_01720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR5098368.1"
                     /translation="MYVLVVTIEIKAGFKDKFIAAMLDDARGSVGKEPGCVRFDVIQD
                     EKVANRIYLYEVYKDRAAFDFHMTTPHFLTWNETVKDWLASPPVVGAGPSIFPADHDW
                     NRKWKAR"
     gene            2705..3514
                     /locus_tag="AC16G0_01725"
     CDS             2705..3514
                     /locus_tag="AC16G0_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR5098369.1"
                     /translation="MKVRGMVVVGVLVLLALAWAPVASAQQTSRLDDIVKAGVVRIGV
                     FTDLEPYGFLDASGKNVGYDVDIGTELAKRLGVKTEFVQVAGPTRVPSLVAGKVDVVI
                     ADFTRTLERSKLIAFTDPYIVVGNTYMVLAKRAELKTAADLNKPSIKVAISRGGTSEQ
                     TVPASLPNATLVRFDNYNDILTAVRTGKADVVSEDNIFTGQQVAKSPKEWKVIPGLFS
                     HEDICIGVPKGDFVWQNWVNLFVHEINVDGTNKALFKKWFKADVMKLQPSY"
     gene            3596..4855
                     /locus_tag="AC16G0_01730"
     CDS             3596..4855
                     /locus_tag="AC16G0_01730"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR5098370.1"
                     /translation="MTQKFVLDCSYCGHRHEASYLGMRCERCDGPLIVNTDLGPLKTG
                     PAARTNLHDSSLPGMWTYRSLLPIADRSAVVSLGEGNTPLTLAGRLAQQLGIDRLYLK
                     DETRNPTGSFKDRMLAVGVTRARELGKRTIAVQSSGNVAAAAAAYAAKAGLQAKIFVP
                     RTAPEEKLVQVMMYGADLFRIDHSSPTDIFELLRWACEEFDWYLASTAAIFNPFTLEG
                     AKTIAYEIAEQTGFDLPEWIIVPVGGGGNIGSLWRGFNDLRALGLVARLPRMVGVQAA
                     GCAPIVEAIRLGRTAKEALTWRWPAIDTVAGAIADDVVFDAHIALPAVRESQGTALAV
                     PDDDTLAMERLLASTEGIFVEPASATTLAGLKKLLDQGLIQRRDRVCCVLTGLGFKDM
                     ASARKLVPPIETVPLSREAILARVRRP"
     gene            4863..5534
                     /locus_tag="AC16G0_01735"
     CDS             4863..5534
                     /locus_tag="AC16G0_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR5098371.1"
                     /translation="MTGSYTFDFLAVLRYGPELLKGAWVAALLSILTFILGTILGIFG
                     AIGRISRRRAVYAVSSFYVELFRNTPLLVQMYIFYYGLDALSINLSAFTAALLAMSLN
                     SGAYETEIIRGGIIAVGKGQLEAAFSIGMTYAQTLRKVVIPYALQVIFPPLGNQFIGI
                     ILGSSVASIITVPEITYVALNIGSRSYRDFETFLVIWVIYLALNLALSALFRLSGRMA
                     FRPIF"
     gene            5547..6200
                     /locus_tag="AC16G0_01740"
     CDS             5547..6200
                     /locus_tag="AC16G0_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR5098372.1"
                     /translation="MENQPVVIQYAVFFLKAAGITLGISALSLLVGSVIGFGFSLLRI
                     SRQRIFSALTFAYAEIFRGTPILVQLFLVYFGLPIVFRINVSAFTTVAVSMILYTAGY
                     MTEIFRAAIQAVDRGQWDASYTIGMTYTQALLKVIVPQAMRIAVPPTLGQFISILKDS
                     SLASIIGFMELTKAGMAVRAATSSTLLAFTIVTCLYFAICYSLSVASRRVELRVKKA"
     gene            6220..6954
                     /locus_tag="AC16G0_01745"
     CDS             6220..6954
                     /locus_tag="AC16G0_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR5098373.1"
                     /translation="MPILRTENLNKHYDRLHVLKNVSFSVERGETLIILGPSGSGKST
                     LLRCLNQLERIDAGTIYFEEQPLVYDRRSVYRNRRNFGMIFQSFNLFPQKTALENITL
                     GPITVAKVPRAEAEREALAFLEKVKLGDKAHAYPAKLSGGQQQRVAIARALAMHPKVL
                     LFDEPTSALDPETIGDVLEVMRKLAQEHTTMIVVTHELGFAREIAERIIFMDAGEIVE
                     EGLSQQLLTRPKSARLTAFLDAVIHK"
     gene            6968..8128
                     /locus_tag="AC16G0_01750"
     CDS             6968..8128
                     /locus_tag="AC16G0_01750"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR5098374.1"
                     /translation="MKITDVRTTIVSIPFKKPEIWSMGRRVGVSNVIIEIETDTGLIG
                     LGESIGFPSCDVIKPIIDSWKDDLVGADPLRTEALMQRLEAQGGWQHFDHTGNVARAG
                     VDIALWDLVGKAAGLPLYVIFGGLVQESMSFMYFLPRGPIREMTAQAARGVKEGWETI
                     YIKVGVDIEEEIEVVRAIRKTVGPRPKIRVDANEAWRRYDALRFIRAVEPFGIEFFEQ
                     PILKEDFDGLKWLRSMTSVPIAANQSSWTEQDILKLIRQEAADIMLMDPYQIGGLARF
                     ARAAWIAQVAGVAAVRHSWCELGIGMYASMHVMAASANFPMANQAYPEYLSDDVCKGD
                     LFRFRKGRLSILHGPGIGVELDRRKLEKYARVYRQEGEVSAYAPHEGARKKP"
     gene            8797..9399
                     /locus_tag="AC16G0_01755"
     CDS             8797..9399
                     /locus_tag="AC16G0_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit B"
                     /protein_id="HIR5098375.1"
                     /translation="MGDSLTYLVLVASAVVCAFFAMTSRRMLTSTLFLAGVSAAVALT
                     LYALGAYQVAVIELSVGAGLVTVLLVYAISVVGDDATDAVSVIPKPIAVVLAVVATVL
                     LGRMGLSLASNGTSGDFPPLLSSLWEQRVLDVWIQMVLIFAGVLGILGLLAEGKRAKH
                     HTEPLKIGTHRTVKVIPATACGFPTVTPRKVEEIQEEVHV"
     gene            9396..9707
                     /locus_tag="AC16G0_01760"
     CDS             9396..9707
                     /locus_tag="AC16G0_01760"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HIR5098376.1"
                     /translation="MTLSPFHVILAGAIGLLGVGCYGLLITRNLIKVVIALQVLVKAV
                     VVALVLAGQSGGQPGIGQSLAATVIVADTMVAVVGMALAVQIQRRFGGVDVSAISTLR
                     G"
     gene            9711..>11123
                     /locus_tag="AC16G0_01765"
     CDS             9711..>11123
                     /locus_tag="AC16G0_01765"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit 5 family
                     protein"
                     /protein_id="HIR5098377.1"
                     /translation="MAQWLILLTIGLPWLGALATWLTRNENERAQHTLAVGFSVAAGL
                     SALTLISQAGSGVALRIPIGGLFGDMTFVADGLGAFLAAVATVVGSLAVIFSVDYMHG
                     DPQLGRYYSQVLLFIGAMAGLVLSNSLLFLFFFWEITALCSYALISYYNDDPKAVSAG
                     IEALIMTQVGGVGLLGGALLVYASLGTYEIDAFLGAAGTMLPVSLAWMAFGFLVAAAA
                     KSAQFPFQTWLPDAMEAPTPVSALNHAATMVNAGVYLLARFYPAFAPVPGWKTAVVAV
                     GVISALITACMALVAPDLKRTLAYSTVSQLGYMVYAVGVGGILASSFHLFSHAIFKAL
                     LFLGAGAVIHAVGTRDMGKMGALGARMPFVRTVFVIGALSLAGLPILNGFWSKELVLE
                     AGLTGGPAWALWGMLVGAGLTACYTFRMVWLVFYGEARSKLHAHDATLAMRISLSLLA
                     LGTLTSWLLAGSFGHLLESTL"
BASE COUNT         1915 a         3580 c         3466 g         2162 t
ORIGIN      
        1 agggccgcgc gggtctgcga gccgcccaca ggggtacgcg ccgcgcccgc cacatttgcc
       61 cgcgcctcgg gccaggtcga tatggcgtcg cccatcccgg cgaccagaaa tcgcaccggg
      121 gctttcacga tgactgccgt gtccaccagg accagggccg gattggcacg aagcctggcc
      181 acccggacct ggacgtgatt gtcgtcatag agaacggcga cccggctcgt gggagcgtcg
      241 gtcgaggcga tggtggggac catggccgaa ggcaggcgca agctctctgc catcagcgtc
      301 cccgtgtcca gggacttccc gccgccggca gcgacgatgg ctgccggggc ccaggccttg
      361 gcagcctcgg cgagcgtggc taccgtggcc tctgtacact cgcccggaaa gcgctcggct
      421 cgccaggcca gcccctctgc ccggcaagcc tcggccagcg ccggctccac atcggcccag
      481 acgttcggat ccacgacccc aaagatgcgc ccgcccaggc gggcgaggtg tgggctcgct
      541 gtcttcagga tccccggcgc ctggacataa gtgccgggag cggcgaagat cataggaagg
      601 cgttcggcca tgatggtcct cctggatagc gtcagatgcg gttgtcaacc atcgaccgtc
      661 aacctgccag gctaaatcgc ccccgaagac ttgagggcgg cgattgcatc cggggagtag
      721 ccgaggagat ctccaaggac gacctcgttc gccgcaccca tggggagacc ggggaagcgg
      781 atcttgccgg gggtgccgga catccggatg ggagcctcgg ggaacgggat gtctccttcc
      841 gagacgggat cgaacatgga gatgatgctg cccctcgcct tgatattggg atccttcgcg
      901 atgtcctcca tgctgtagat cggaccgata gtcacgcccg ccttctcgca ctcctccagc
      961 actgtctgga tcggtcgccc ctttatccag ctcccgatga cgtggttcaa gagctgccgg
     1021 ctcgcctgct gggtccgctc ctgattgttc cggaagccgg gctcctgctt cagatccggc
     1081 cggcccacga ggtccatcag ccgctcgaag gtggcctggg agctggcgga gaggacgacc
     1141 cacttcccat ccttggtctg gtagctgttc cgcggcgacg tgtaatgggc ttcgttcccc
     1201 atcggtgtcg gggatgtccc cgacagcacg taggtaagaa aatccgctcc catgagggaa
     1261 aagagcggct cgtagagcga aaggtccaac tcctggccgc caaactcccc ccgcttgctg
     1321 tggcggagcg ccaggattac gcccagcgcc aagtgcaagc cggcgatcat gtccgccagg
     1381 ggaagcgggg agttcgtggg cgggccgccc ggatgggcgt tcatgtaggt gaatccgctc
     1441 atggcctcgg ccagcgtgcc gaagcccggc ttggccgcag aaggaccggt ctggccgtag
     1501 cccgagacgc gcccgatgat caatcccttg ttgatctccc agagcttcgt ggcgggaaat
     1561 ccaagccggt cgagggtccc ggggcgcatg ttctcgagga gaacatcgga ccgctccacg
     1621 agccgggcga agacctgctg ccccttcggg tgcttcaagt tcagcgtcac cggaagcttg
     1681 ttccgcccgg cgagtagcca gaagggctga tgctccccga ccatcgccca gtaccggatg
     1741 gcgtctggca cgctgggcgg ctcgatcttg atcacctctg ccccatagtc ggcgagcgtc
     1801 gtggccccga agggggcggc gaccaccgac ccaaagtcga tgatccggat gtctttgagc
     1861 ggggccttcg atccctccgc ctcaccccgc tgcgcccgga gaaactccgt gagatacttc
     1921 ccgtccatgg ggccccctta gcgtgccttc catttccggt tccagtcgtg gtcggcggga
     1981 aagatgctgg ggccggcgcc aacgaccggg ggtgatgcca gccagtcctt caccgtctcg
     2041 ttccacgtga ggaagtgagg cgtcgtcatg tggaaatcga acgccgcccg atccttgtag
     2101 acctcgtaca gatagatgcg gtttgccacc ttctcatcct ggatgacgtc aaagcgcacg
     2161 cagccgggct ccttgcccac cgagccgcgg gcgtcgtcca gcatcgccgc aatgaacttg
     2221 tccttgaatc ctgccttgat ctcgatggtc accacgagga cgtacatgcg gcctcctttc
     2281 ctccccttaa ccagacgcac ccggccgcac tccagccatg cacaggctgc tcccacggct
     2341 cgggctgttt catccattcc cgcggcgaga cgctacccgc gagccccgag ggagtcaagg
     2401 ggaaagcgcc gggaggcttg cgcggaaaat ggacaaggtg tacgctcagg ggcgtctcag
     2461 gtctttcgtc ctggaagtcc aaacccgcac gtcagccagc gatctacggc tccaaccatc
     2521 ctatcgaatc ggttgccggg tgatggtttc ggtaggccgg cttcaagaac ttatcgggaa
     2581 ggaggtgggg cagcgggggt cgcaggcccc cattagagat ggcgactccc aggggtgtgg
     2641 cggctcggac actccgagcg acgggaagca atcggattct cgtacggagg acaaggaggt
     2701 tgccatgaaa gtccgaggca tggtcgtggt tggagtactg gtactcctcg cgttggcgtg
     2761 ggcgccggtg gcctctgccc agcagacgag ccgcctggac gacatcgtga aggccggtgt
     2821 ggtgcggatc ggggtcttca cggatctgga gccgtacggg ttcctggacg ccagcggaaa
     2881 gaacgtgggc tacgacgtgg acatcggcac ggagttggcg aagcggcttg gcgtcaagac
     2941 cgagttcgtt caggtcgcgg gtcccacccg ggtcccctcc ctcgtggccg ggaaagtgga
     3001 cgtggtcatc gccgatttca cccggaccct ggagcgcagc aagctcatcg cgttcaccga
     3061 cccctacatc gtggtcggta acacctacat ggtgctggcc aagcgggccg aactcaagac
     3121 cgccgccgat ttgaacaagc cctcgatcaa ggtcgccatc tcgcgtgggg ggacgtctga
     3181 gcagacggtt cccgcgtccc ttcccaatgc caccctggtc cgcttcgaca actataacga
     3241 tatcctgacg gcggtccgca ccggcaaggc ggatgtcgtc tccgaagaca acatcttcac
     3301 cggccagcag gtcgccaaga gccccaagga gtggaaagtg atccccggcc tcttctccca
     3361 cgaggacatt tgcatcggcg tccccaaggg ggatttcgtc tggcaaaact gggtgaacct
     3421 cttcgtccac gagatcaacg tggatggcac caacaaggcg ctcttcaaga agtggttcaa
     3481 ggccgacgtc atgaagctcc agcccagcta ttgacgacca actgcatccg gggaagggag
     3541 gatggacgcc attctccctt cccgatttcg atctcccctc agacgaggtc cgccaatgac
     3601 ccagaaattc gtgcttgatt gcagctactg tggccaccgg cacgaggctt cctacctcgg
     3661 gatgcgctgc gagcgctgcg acgggccgct gatcgtcaac actgatctgg gtcccttgaa
     3721 gaccgggccg gcggcaagaa cgaacctgca tgattccagc cttccgggga tgtggaccta
     3781 ccgctcactc ttacccatcg cggaccggag cgccgttgtg tccctggggg aagggaatac
     3841 cccactgact ctggcgggtc ggctggccca gcaacttgga atcgacaggc tctacctgaa
     3901 ggatgagacc cgcaacccca cgggatcctt caaggatcga atgcttgccg tgggggtcac
     3961 gcgggcgcgc gaactcggca agaggaccat tgccgtgcag tcctcgggga acgtcgccgc
     4021 ggcggcggcg gcctacgcgg ccaaggccgg gctccaggcc aagatcttcg tcccccgcac
     4081 cgccccggaa gaaaaactcg ttcaggtcat gatgtacggg gccgacctct tccgcatcga
     4141 ccacagttcc cccacggata tcttcgagtt gctgcgctgg gcctgtgagg aattcgactg
     4201 gtacctcgcc tccaccgccg ccatcttcaa tccgttcacc ctggaaggcg ccaagaccat
     4261 cgcctacgag atcgccgagc agacaggctt cgacctgccg gagtggatca tcgtgcccgt
     4321 gggaggcggc ggcaacatcg gaagtctgtg gcggggattc aacgacctcc gggccctcgg
     4381 cctggttgcc cggttacctc gcatggtcgg cgtgcaggcg gccggctgcg cccctatcgt
     4441 cgaggcgatc cggctgggtc ggacggcgaa ggaggccttg acgtggcgct ggcccgccat
     4501 agacaccgtg gccggcgcca tcgccgacga tgtggttttc gacgctcaca tcgccctgcc
     4561 tgcggtgcgg gagtctcagg gtacggcgct ggccgtgccc gacgacgaca cgctcgccat
     4621 ggagcgtctg ctggcctcca ccgaaggtat ctttgtcgag ccggccagcg ccacgaccct
     4681 ggccggcctg aagaagcttc tggaccaggg actgatccag cgacgggacc gcgtctgctg
     4741 cgtcctcacc gggctcggct tcaaggacat ggcgtccgcg cggaagctgg tgcccccgat
     4801 cgagactgtt cccttgagtc gcgaggccat cctggcccgc gtgcgtcgcc catgaggtct
     4861 gaatgacggg gagctacacc ttcgacttcc tggcggtgct gcgctacggg ccggagctgc
     4921 tcaagggggc gtgggtggcg gcccttctga gcatcctgac cttcatcctc gggaccatcc
     4981 ttgggatctt tggcgctatc gggcgcatct cccgccgacg ggccgtgtat gcggtcagca
     5041 gcttttacgt ggagctgttt cgcaataccc cgctcctggt ccagatgtat atcttctact
     5101 acggcctgga tgccctctcc atcaatctca gcgccttcac cgccgccctg ctggccatgt
     5161 cgctcaactc cggcgcttat gagacagaga tcatccgcgg gggcatcatc gctgtcggca
     5221 aagggcagct ggaggcggca ttctccattg gcatgaccta cgcccagacc ctgcggaagg
     5281 tggtcatccc ctacgccctg caggtgatct tccccccact tggcaatcag ttcatcggca
     5341 tcatcctggg atcctcggtc gcctccatca tcaccgtccc cgagatcacc tacgtcgccc
     5401 tcaacatcgg ctcgcgctcg taccgggact tcgagacctt cctggtgatc tgggtgatct
     5461 acctggcgtt gaacttggcc ctctcggccc tcttcaggct gtcggggagg atggcgtttc
     5521 gtccgatctt ctgagaggtt tcgccggtgg agaatcaacc ggtcgtcatc cagtacgccg
     5581 tcttcttcct gaaggccgca gggatcacct tgggcatctc ggcgctctcg ctcctcgtgg
     5641 gttcggtcat cggcttcggc ttcagcctct tgcggatctc gcggcagcgg atcttctcgg
     5701 cgctgacctt cgcctatgcc gagatcttcc gcgggacccc catcctggtg cagctcttcc
     5761 tcgtgtattt cggcctgcca atcgtcttcc gcatcaacgt ctccgccttc accaccgtgg
     5821 ctgtgagcat gatcctctac accgccggct acatgaccga gatcttccgg gcggccatcc
     5881 aggcggtgga ccgcggccag tgggacgcct cgtacacgat cggcatgacg tacacccagg
     5941 ccctgctcaa ggtcatcgtc ccccaggcga tgcgcatcgc ggttccgccc accctcgggc
     6001 agttcatctc gatcctcaag gactcctccc tcgcctccat catcggcttc atggaactga
     6061 ccaaggccgg tatggcggtc cgggcggcga cttccagcac gctcctcgcc ttcaccatcg
     6121 tcacgtgcct gtatttcgcc atctgctatt cactctctgt cgcgtcccgg cgcgtcgagc
     6181 tccgcgtgaa gaaggcctga ccgctcgtca gggggatcca tgccgatcct gcgaaccgag
     6241 aacttgaaca agcattacga caggctgcat gtgctcaaga acgtctcctt cagcgtggag
     6301 cggggggaga cgctgatcat cctggggccc agcggcagcg ggaagagcac gctcctccgc
     6361 tgcttgaacc agctcgagcg catcgacgcc gggacgatct acttcgagga gcaacccctc
     6421 gtctatgacc gccgctcggt gtaccgcaac cggcggaact tcgggatgat cttccagtcg
     6481 ttcaacctct tcccgcagaa gacagccctg gaaaacatca cccttgggcc catcaccgtg
     6541 gcaaaggtcc cgcgggcgga ggccgaacgg gaggccctgg catttctgga aaaggtcaag
     6601 ctaggcgaca aggcgcacgc ctatccggcc aagctgtccg gcggccagca gcagcgggtg
     6661 gccatcgccc gggcactggc gatgcacccg aaggtcctgc tcttcgacga gccgacctcc
     6721 gccctggacc ccgaaacgat cggcgacgtc ctggaggtca tgcgcaagct ggcccaggaa
     6781 catacgacca tgatcgttgt gacccacgaa ctgggcttcg cccgggaaat cgcggagcgc
     6841 atcattttca tggacgctgg agagatcgtg gaagagggtc tgtcgcagca actcctcacc
     6901 cgaccgaagt cggcccgact gaccgcattc ctcgacgccg tcattcacaa gtaggcaagg
     6961 aggcagcatg aagatcaccg atgttcggac cacaatcgtg tccattccct tcaagaagcc
     7021 cgagatctgg tccatgggaa ggcgcgtggg cgtctccaat gtcatcatcg agatcgagac
     7081 ggacacgggg ttgatcggcc tgggcgaatc tatcgggttc ccctcatgtg atgtgatcaa
     7141 gccgatcatc gattcctgga aggacgacct cgtcggagcg gatcccttgc gcaccgaggc
     7201 gctcatgcag agactggagg cgcaaggggg ctggcagcac ttcgaccaca ccggcaacgt
     7261 cgcgcgcgcg ggggtggaca tcgcgctgtg ggacttggtg ggaaaggctg cggggctccc
     7321 cttgtacgtc atctttggag gcctagtaca ggagagcatg tctttcatgt acttcctacc
     7381 ccggggaccg atcagggaga tgaccgctca agccgctcgg ggggtcaagg aaggctggga
     7441 aactatttac atcaaggtcg gcgtggatat cgaggaagag attgaggtcg tgcgggccat
     7501 tcgcaagacc gtgggcccac gacccaagat ccgcgtggac gccaatgaag cctggaggcg
     7561 ctacgatgcg ttgcggttca tccgcgccgt ggagcccttc gggatcgagt tcttcgagca
     7621 gccgatcctc aaggaggatt tcgacggact caaatggctg cgcagcatga cctccgtccc
     7681 tatcgccgcc aatcaaagct cctggacgga gcaagacatc ctgaagctca tccggcaaga
     7741 ggccgcagac atcatgctga tggaccccta ccagattggc ggcctggccc ggttcgcccg
     7801 tgcggcctgg atcgcccagg tggcgggtgt ggcggcggtc aggcacagct ggtgcgaact
     7861 ggggatcggg atgtatgcct ccatgcacgt catggccgct tcggcgaact tccccatggc
     7921 caaccaggct tatccggagt acctctcgga tgatgtctgc aaaggcgatc tcttccggtt
     7981 ccgcaaaggc cggctgtcga ttctgcacgg ccccggcatt ggcgtcgagt tggaccggag
     8041 gaagctggaa aagtacgcgc gcgtttaccg tcaggaagga gaggtctcgg cgtatgctcc
     8101 gcatgaaggc gcgaggaaga agccctgacc gtcgcgggga tgtcccgtga tcgagcttcc
     8161 tcctcttggt ggccctgcgc gagattgatg tcccgcaagg cagagtgttg gttggattcg
     8221 gaccctccaa tcttgcgcct gggccggcgg ccggtcggct ttaacgaccg tcgacccagg
     8281 cgatgccttc tcccttggta gaagctagta ggagcaaaat ccccttaaag taaaggccaa
     8341 agacggtacg ccgccacccc cgtgacccaa atagcaacct ctccgttctc ttgaggcctg
     8401 cttttccgac ccaactcatg ccttccggtc ccacctttcg acgacaactc gtcccattcg
     8461 gctgatcaag ccccaggcac cactcggacg gcctttgatg ccaaaggatc ttggcgccta
     8521 tctgctttgt gtggcaacgt gtcccagaga aaatgtaacg gattgaattc gtattttttt
     8581 ctatattaac aatgagttct gcagatcaat atgttgcaat gtttaccagg aataaaacaa
     8641 agtggctctt cacttgcaac tccatgtaca tgaatctcgt ggtctaacca gcagaccaag
     8701 tagcttattt ggagcgattg aatccgtttc ccgacgacgg atgtttctgg tttctatcaa
     8761 cgctgattcg catatacatc ggagggtaga tgcgccatgg gtgatagcct gacctacctg
     8821 gttttggtcg ctagcgccgt ggtctgcgcc ttttttgcca tgacgtccag gcgcatgctg
     8881 acgtccacct tgttcctagc tggtgtgagc gcggcggtgg cgctcacgct ctacgcgctg
     8941 ggcgcctacc aggtcgccgt tatcgaattg agtgtgggcg cggggctcgt caccgtgctg
     9001 ttggtttatg ccatcagcgt ggtgggcgat gacgccaccg atgcggtctc cgtcatcccg
     9061 aagcccatcg ccgttgtcct ggctgtcgtg gcgacggtgc tgctcggccg gatgggccta
     9121 tccctcgcaa gcaatgggac gagcggggac ttcccgccgc tgctctcgag cctctgggaa
     9181 cagcgggtcc tggatgtctg gattcaaatg gtgctgatct tcgcgggcgt gctgggcatc
     9241 ctgggactcc tggccgaggg gaaacgtgcc aagcaccata cagaaccgct caagatcggg
     9301 acccatcgga cggtcaaggt cattccggcc acggcatgcg ggttcccaac cgtcactccc
     9361 cggaaggtcg aggagatcca agaagaggtg cacgtatgac gctctccccg tttcacgtca
     9421 tcctggccgg cgccattggc ttgctcggtg taggctgtta cggcctcttg atcacccgca
     9481 acctgatcaa ggtcgtgatc gccctgcagg tgctggtgaa ggccgtggtg gtggcattgg
     9541 tcctggcggg acagtccggc gggcagccgg ggatcgggca gagcctggcg gcgaccgtga
     9601 tcgtcgccga caccatggtg gcggtggttg gaatggcgct ggcggtccag attcaacggc
     9661 gattcggcgg cgtggacgtc tctgcaatct ctaccctgcg aggctgaggc atggcacagt
     9721 ggctgattct tctcaccatc ggtttgccct ggctgggggc gctggcgaca tggctcacgc
     9781 gaaatgagaa cgagcgagca cagcacaccc tggccgtcgg cttcagcgtg gccgcgggac
     9841 tctcggccct gaccttgatc tctcaggccg gaagcggcgt ggccctccga atccccatcg
     9901 gcggcctctt cggtgacatg accttcgtgg cggacgggct tggggccttt ctggctgcgg
     9961 tggccacggt cgtggggagc ttggcggtca tcttctccgt ggactacatg cacggcgacc
    10021 cgcagctcgg ccgctattac tcccaggttt tgctcttcat cggggccatg gccgggctgg
    10081 tcttgagcaa cagcctcctc ttcctcttct tcttctggga gatcacggcc ctctgctctt
    10141 acgccctcat ctcgtactac aacgacgacc ccaaggccgt ctccgccggc atcgaggctc
    10201 tcattatgac ccaggtgggc ggggtgggcc tcctgggcgg ggccttactc gtgtacgcca
    10261 gcctggggac atacgagatc gacgccttcc tcggtgcggc cggcacgatg ctgcctgtaa
    10321 gcctcgcctg gatggccttc ggcttcctgg tggccgcggc ggccaagtcg gctcagttcc
    10381 ccttccagac ctggctacct gatgccatgg aggctccgac gcctgtctcg gccctgaacc
    10441 atgccgccac catggtgaac gccggcgtct atcttctggc gcgcttctac ccggccttcg
    10501 cccctgtccc cggctggaag acggcggtag tcgccgtggg cgtgatctcc gccctcatca
    10561 ctgcctgcat ggcgctggtc gcgccagatc tgaagcgtac gctggcctac tcgacggtca
    10621 gccagctcgg ctacatggtg tacgcggtgg gtgtcggcgg gatcctggcg agcagcttcc
    10681 acctcttcag ccacgctatc ttcaaggccc tcttgttcct cggggcgggt gccgtcatcc
    10741 acgccgtggg gacgcgggac atggggaaga tgggggcctt gggggcccgc atgcccttcg
    10801 tgcgcaccgt cttcgtgatc ggggccctct ccttggccgg cctgcccatc ctgaacggct
    10861 tctggagcaa ggagctggtc ctggaggccg gcctcaccgg cggtccggcc tgggccctgt
    10921 gggggatgct cgtcggggct ggattgacgg cctgctacac cttccgcatg gtgtggctgg
    10981 tcttctacgg cgaagctcgc agcaagctac acgcgcacga cgccaccctg gcgatgcgca
    11041 tctcgctcag cctgctcgct ctcgggacgt tgaccagttg gctgcttgca gggtccttcg
    11101 gacacctgct ggaatccacc ctg
//
LOCUS       DBNGZC010000092         2683 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2551818, whole genome shotgun sequence.
ACCESSION   DBNGZC010000092 DBNGZC010000000
VERSION     DBNGZC010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2551818"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..1074
                     /locus_tag="AC16G0_01770"
     CDS             46..1074
                     /locus_tag="AC16G0_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR5098378.1"
                     /translation="MVFDWIFGMFSNDLAIDLGTANTLIYVKGRGIILSEPSVVAIKK
                     GTNQVLKVGREAKEMLGRTPGSIVAIRPLKDGVIADFDVTEQMIRAFIVKTHNRKTLV
                     RPRVIIGVPSGITQVEKRAIRDSADQAGAREVYLIEEPMAAAIGAGLPVQEPTGSMIV
                     DIGGGTTEVAVISLSGIVYSQSVRIAGDEMDEAITQYLKRKYNLLVGERTSENIKIQI
                     GSAYPFDEPRKMEIKGRDLVDGIPKTLTINDSDIREALHDPIFAIVDAVKTALERTPP
                     ELAADIAEKGIVLAGGGSLLHGLDTLIALETHLKVRVADDPLSCVVLGTGKVLDELDL
                     LKTVCLES"
     gene            1188..2057
                     /gene="mreC"
                     /locus_tag="AC16G0_01775"
     CDS             1188..2057
                     /gene="mreC"
                     /locus_tag="AC16G0_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR5098379.1"
                     /translation="MSTIVRLLARYRRAIILATALILAFLLMTLQVRQERAVVAVTRQ
                     AILLVMTPFIRVTAVIVDSVTDVWRDYVDLRDLRGENKRLRQEVGTLTRRIEQLEEQA
                     LETQRLQRLLALRQVPQAESLTARVEGKDATNWFKTILVDRGSLDGVRRNQPVVAPDG
                     LVGRVVEVTPASAKVQLLTDPISAVGGLVQRTRVTGIISGNLGAGTRVRYLPLMSDVA
                     VGDLVVTSGMGGVFPKGIPIGRITAVERRSGALFQEASLQPSVDLSRLEEVLILTAPP
                     PGEGVGTASGSQP"
     gene            2054..2536
                     /gene="mreD"
                     /locus_tag="AC16G0_01780"
     CDS             2054..2536
                     /gene="mreD"
                     /locus_tag="AC16G0_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR5098380.1"
                     /translation="MKWFLPGLLACTITQAVLLSPLSLGGVRPDLFLLLVFFLSLRCG
                     EETATLQGFLIGLCQDALSGGPLGLRTFTYSLLGFLTSRLSHDLYTENLLAQFWLLLG
                     GCTAAGGITLVLLTFFLGPPPLLTTFLWVIAPEALYTAVVGLLLLRLPHLRAAVTRPA
                     "
BASE COUNT          442 a          897 c          865 g          479 t
ORIGIN      
        1 agcaactcta gggatgtact ccttggagga ccgaagggag acgcaatggt gtttgactgg
       61 atatttggga tgttctccaa cgacctcgcc attgatctcg gcacggccaa cacgctgatt
      121 tatgtcaagg gccgtgggat catcctgagc gagccgtcgg tggttgcaat aaagaaggga
      181 acgaaccagg tgctcaaggt cgggcgcgag gccaaggaaa tgttgggccg cacccccggc
      241 agcatcgtgg ccatccgccc cctgaaggac ggcgtcatcg ccgacttcga cgtgaccgag
      301 cagatgatcc gcgccttcat cgtgaagacc cacaaccgca agacgctggt gcgcccgcgg
      361 gtcatcatcg gcgtcccctc cgggatcacc caggtggaga agcgggccat ccgcgattcc
      421 gcagaccagg caggcgcccg cgaggtctac ctgatcgagg agcccatggc cgccgccatc
      481 ggcgcggggc tcccggtcca ggaacccacc ggcagcatga tcgtggacat cggcgggggc
      541 accacggagg ttgccgttat ctcactctcc ggcatcgtct acagccaatc ggtccggatc
      601 gccggggatg agatggacga ggccatcacc cagtacctga agcggaagta caacctgctg
      661 gttggcgagc ggacgtcgga gaacatcaag atccagatcg gctccgccta tcccttcgac
      721 gagccgcgca agatggagat caaggggcgc gacctggtgg acggcatacc caagaccctc
      781 accatcaacg acagcgacat ccgggaggcc ctgcacgacc ccatcttcgc catcgtggac
      841 gcggtcaaga ccgcgctgga gcggaccccg cccgagctgg ccgcggacat cgccgagaag
      901 ggcatcgtcc tggccggggg cggttctctg ctccacggac tggacacgct gatcgctctg
      961 gagacgcatc tcaaggtccg ggtggccgac gaccccctgt cctgcgtcgt cctgggaacc
     1021 ggcaaggtcc tggacgagct tgacttgttg aagacggtct gcttagagtc gtaggtatcc
     1081 gacgggaggc cggacctccc ccggaagcgg atggctgcgc gcggcgccca ctcctgggtt
     1141 ccgaggaaaa ctgacatggg ccgcccagga catccgaggt atatctcgtg agcacgatcg
     1201 tgcgtctctt ggcccggtac cgccgggcca tcatcctggc cacggcgctg atcctggcct
     1261 tcctgctcat gacgctgcag gtgcgtcaag aacgggccgt cgtggccgtc acgcggcagg
     1321 ccatcctcct cgtcatgacc cccttcatca gggtgacggc cgtgatcgtg gacagcgtca
     1381 ccgacgtgtg gcgcgactac gtggacctcc gcgatctccg gggggagaac aagcgcctgc
     1441 gacaggaggt ggggaccctc acccggcgca tcgagcaact cgaggaacag gcgctggaga
     1501 cccagcgcct gcagcgtctg ctcgcgctgc gccaggtccc tcaggccgag tcgttgaccg
     1561 cccgggtcga gggcaaggat gccaccaact ggttcaagac gatcctggtg gatcggggca
     1621 gcctggacgg agtgcgccgc aatcagccgg tggtggcccc ggacggactg gtgggtcggg
     1681 tggtggaggt gacgcccgcc tcggccaagg tgcaactcct caccgatccg ataagtgctg
     1741 tgggcggtct ggtgcagcga acgcgagtca ccggcatcat ctcgggcaat ctgggtgcgg
     1801 gcactcgagt gcgctacctg ccgctcatgt ctgatgtcgc cgtcggcgac ttggtggtga
     1861 cctcgggcat gggtggcgtg ttccccaagg gcatccccat tgggcgcatc accgccgtcg
     1921 agcgacggag cggcgccctt ttccaggagg cgtcgctcca gcccagcgtg gatctgtccc
     1981 gcctggagga ggtcctgatc ctgaccgcgc ctccgcccgg cgaaggggtt gggacggcga
     2041 gcgggagcca gccatgaagt ggttcctccc cggtctcctc gcctgcacca tcacgcaggc
     2101 cgtgcttctg tctcccctca gcctgggggg cgtccgaccc gatctgttcc tgctcctggt
     2161 gttctttctt agcctgcgct gcggggaaga aacggcgacg ctacagggat tcctcatcgg
     2221 gctgtgccag gacgcgctct ccgggggacc cttgggcctg cgcaccttca cttacagcct
     2281 actgggattc ctcacgtcgc gtctcagcca tgatctgtac accgagaacc ttctggcgca
     2341 gttctggctc ctcctcggag gctgcaccgc agccgggggc atcaccctcg tgctgctcac
     2401 gttcttcctg ggacccccac cgctcctcac gaccttcctc tgggtgattg cacccgaggc
     2461 gctctacacg gcggtagtcg gcctcctgct cttacggctg ccccaccttc gcgccgccgt
     2521 cactcgaccc gcatgagcct ggatccactc ccgctgagtc ccgcccgggc cctcccatcc
     2581 cgcttgcgga agttggccct gtgcgtgggc attggtgtgc tggtgctcct gctgcgtctg
     2641 tggtacctcc agatcctgga gggcggattc tactctgccc tct
//
LOCUS       DBNGZC010000093         3909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_301314, whole genome shotgun sequence.
ACCESSION   DBNGZC010000093 DBNGZC010000000
VERSION     DBNGZC010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_301314"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..1228
                     /locus_tag="AC16G0_01785"
     CDS             311..1228
                     /locus_tag="AC16G0_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HIR5098381.1"
                     /translation="MRLAVTGSRGLLGSALVPFMLTGGHQVIRLVRGSTRGSEEASWD
                     PARGLADPASLEGVDAVIHLAGENIAAARWTRERKAEIRRSRVEGTRRLCEGLARFPR
                     PPKALVCASAIGYYGDRGDEILREDSPPGTGFLAEVCQLWESAAVPASQAGIRVVHMR
                     FGMILTPAGGALRKMLVPFRLGLGGKMGNGGQYVSWIALDDATLAVHHVLSNDSVRGP
                     VNAVAPAPVTNAEFTRTLARVLSRPALATLPAFAVRLAFGEMADALLLASQRVVPSRL
                     QASGFGFRFPTLEGALRHLLGRRDRDRVG"
     gene            1264..2757
                     /locus_tag="AC16G0_01790"
     CDS             1264..2757
                     /locus_tag="AC16G0_01790"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: protein motif:HMM:NF013071.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HIR5098382.1"
                     /translation="MRRLNDRPERSGGDFVLYWIQMYQRAEQNFALSAAIDAANRLGV
                     PVVAHQELAHDLPHANDRIHRFLLEGVTELSKRFTSRGVRYHFHLRQAEGDPRDLVLN
                     LARRAALVVTDDFPAFVVPTESRRIAEQVDVAVLAVDSNGIVPLAAIPGEQYGAYTLR
                     PRLRRLLPDHLRPIPEPRPRRDSLGIRLDLPETRVNPSTMDALIAGCAIDHSVLPSRV
                     YLGGYAQARARLNRFIAGPLRQYAKARNQPGEDRTSRLSAYLHTGQIAVHEVALAVRE
                     APGIPAEDRDAYLEELIVRRELAYNFCRFNPHHRSLAGLPAWARQTLASHENDPRPYV
                     YSSDEFERARTHDYLWNATQAELLTTGAMVGYYRMYWGKKIIEWSRTPAEAQAIMIRL
                     HEKYALDGRDPNTYSNILWCMGKHDRPWFERPVFGTVRYMSLGGMEAKTDVAAYVERV
                     NRWCVEAGRPDLRVTAPAKPTRTKRTQKGRREVAPMPRVDWSYHRPG"
     gene            2770..3066
                     /locus_tag="AC16G0_01795"
                     /pseudo
     CDS             2770..3066
                     /locus_tag="AC16G0_01795"
                     /inference="COORDINATES: protein motif:HMM:NF041106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family (seleno)protein"
     gene            3066..3575
                     /locus_tag="AC16G0_01800"
     CDS             3066..3575
                     /locus_tag="AC16G0_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TspO/MBR family protein"
                     /protein_id="HIR5098383.1"
                     /translation="MGPAKLEQDARKRLAPQAVALGLCLAACFAAAGLGSMATVPALG
                     EWYAGLVKPAWNPPSWVFGPVWTTLYATMAVAAWLVWKRGGPGRPPALILFGVQLALN
                     ASWSWLFFGLRQPGAALAEILILWLAVAATAWAFGRLSRPAALLFIPYLLWVSFAAVL
                     NWALWRLNA"
     gene            3641..>3909
                     /locus_tag="AC16G0_01805"
     CDS             3641..>3909
                     /locus_tag="AC16G0_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015860290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-thiouracil desulfurase family protein"
                     /protein_id="HIR5098384.1"
                     /translation="MTPQTGLPGQDSRPIRVGISSCLLGNEVRYDGGHKRDRYLTDTL
                     SRFFEWVPVCPEVEMGLGTPRETLRLVGEPEAPRLAFAKTGEDQT"
BASE COUNT          617 a         1289 c         1350 g          653 t
ORIGIN      
        1 gcatgatcgg ctcgctcgct gcattgcagg gagggcggtg cgccgtaccc ggtacgtgca
       61 cgccggggtg attgccgtgc cggccgaggc cgtgatcgtg tgctaggtgg ggccgggtgc
      121 tctggaacgc ctgacgccag cgtgagagcg cgcagatgtc gaagcccgtt acatcctccc
      181 catcggtccg ctcgggcggt tggtcgccgg gggcgcagtc caacgaaagc tccgcaggat
      241 tttcgcctat cgtcatgcgg tcacccgggc ggacgtcgag gtccatgggg ggcgagcggg
      301 ggagccggcc atgaggctcg ccgtcacggg ttcacgcggc ctgctcgggt cagccctcgt
      361 accgttcatg ttgacgggtg gccaccaggt catccgactg gtccgcggca gcacgcgggg
      421 aagcgaagag gcctcctggg atcccgcccg cgggctggcg gaccctgcct ccctcgaggg
      481 tgtggatgcg gtgattcatc tggctgggga gaacattgcg gcggcacgat ggacgcggga
      541 gcggaaggca gagatccgcc gcagccgagt cgagggcacc cggcgcctgt gtgaggggct
      601 ggcccgattt cctcgacccc ccaaggcgct cgtgtgcgcc tcggccatcg ggtattacgg
      661 agaccgtgga gacgagattc tccgcgagga cagcccgccc gggaccggct ttctggccga
      721 ggtctgccag ttgtgggagt cggcggccgt gccggcctcc caagccggga ttcgggtcgt
      781 gcacatgcgc ttcgggatga ttctcacgcc ggctggcggc gcactgcgga agatgctggt
      841 gccattccga ttggggctgg gcgggaagat ggggaacggc ggacagtacg tgagctggat
      901 cgccctggac gatgcgaccc tcgccgttca tcatgtcctc agcaacgatt ccgtgcgggg
      961 cccggtcaac gcggtggccc cggctccggt caccaacgcg gagtttaccc gtaccttggc
     1021 gcgggtcctg tcacggccgg ccctggccac gctcccggcc ttcgccgtgc ggctcgcctt
     1081 cggcgagatg gccgatgcgc tccttttggc gagtcaacgc gtggtcccgt cccggctcca
     1141 ggcttcgggt tttggattcc ggtttccgac gctcgagggg gcgctccgac acctcctggg
     1201 caggcgagac cgcgatcggg tggggtaaca aacgtgtccc gtgtagcgat tcccgcagag
     1261 cgcttgaggc ggttgaacga ccggcccgag cgatccggcg gcgatttcgt gctgtactgg
     1321 atccagatgt accagcgcgc cgagcaaaac tttgcgctca gcgctgccat cgacgccgca
     1381 aaccgtctgg gggtgccggt cgttgcccac caggagctgg cgcacgatct tccccacgca
     1441 aatgatcgga tccatcggtt tctcctggag ggcgtgacgg agctttcgaa acggtttacc
     1501 tcccgggggg tccggtatca cttccatctg cggcaggcgg agggcgatcc gcgggacctc
     1561 gtcctgaacc ttgcccggcg agcggcattg gtcgtgaccg acgacttccc ggccttcgtc
     1621 gtgccgacgg aaagccggcg gatcgccgag caggttgacg tggccgtcct ggcggtggac
     1681 agcaacggga tcgtgccgct ggcggcgatc cccggagagc aatacggcgc ctacaccctt
     1741 cgcccgcggc tgcgccggct gctgcccgat cacctgcggc ccattccgga gccccgcccg
     1801 cgacgcgact ctctggggat ccgcctggac ctgccggaga cccgcgtcaa cccgtcgacg
     1861 atggatgcat tgatcgcggg ctgcgccatc gatcactccg tgctcccctc ccgcgtgtac
     1921 ctgggaggat acgcccaggc acgggctcgg ctgaatcggt tcatcgccgg cccgctccgg
     1981 caatacgcga aggctcgaaa ccagccgggg gaggaccgga catcgcgcct gagcgcgtat
     2041 cttcacacag gccagattgc cgtgcacgag gtggccctgg cggtgcgtga ggcacccggc
     2101 atccctgccg aggatcgcga cgcctacctg gaggagctga tcgtgcggcg ggagctggcc
     2161 tacaacttct gccgcttcaa tccgcatcac cgatcactgg cggggctgcc cgcctgggcg
     2221 cgccagacgc ttgcgtcgca cgagaacgac ccgcggcctt acgtgtactc gtccgacgag
     2281 ttcgagcgtg cccggaccca cgactacctc tggaacgcca cccaggcgga gctgctgacc
     2341 accggcgcca tggtcgggta ttaccggatg tactggggga agaagatcat cgagtggagc
     2401 cggacgcccg ccgaggcgca ggcgataatg atccgtctgc acgagaagta cgccctggac
     2461 gggcgcgacc ccaacaccta cagcaacatc ctctggtgca tgggcaagca tgatcggccc
     2521 tggtttgagc gcccggtctt cggaaccgtc cggtacatgt ccctcggcgg gatggaggcc
     2581 aagaccgacg tcgccgccta cgtggagcga gtcaaccgct ggtgcgtgga ggccggacga
     2641 ccggaccttc gcgtgactgc gccggccaaa cccacgcgga cgaaacgcac gcagaagggc
     2701 agaagggagg tcgcacccat gccacgcgtt gattggagtt accaccgccc cgggtgaaag
     2761 acctgcgcca agacgcaggg gttccttgcg cagcacgaca tccagacagc gtcccagatg
     2821 gacgcccgga aaatgccgct gaaggagaaa gaggcgctga gcctgcttcg cgatgtagat
     2881 gagttgtacg tcagcaaggg caagcaggtt gtgcacctgg acctgaagaa ggagaagccg
     2941 gatcgggccg ccctggctgc gctccttctc gggccgaccg gcaacctgcg ggccccgacc
     3001 ctccgcaagg gtcgaacgct catcgtcggt ttcgacgagg cgacgtacgc caagctgttc
     3061 ggatgatggg gcccgccaag ctggagcagg acgcgcggaa aaggttggcc ccgcaagcag
     3121 tggcccttgg gctctgcctc gcggcgtgtt ttgccgcggc gggcctcggc tcgatggcca
     3181 ccgtgcccgc cctcggcgaa tggtatgcgg gccttgtcaa gccagcctgg aacccgccga
     3241 gctgggtctt cggtccggtt tggacaactc tctacgccac gatggccgtc gcggcatggc
     3301 tcgtctggaa gcgcggaggg ccagggcgcc ccccggccct catccttttc ggcgtccaac
     3361 tggcgctcaa cgcttcctgg tcctggctgt tcttcggcct gcgtcagccc ggcgcggccc
     3421 tcgcagagat cctgatcctc tggctggcag tcgccgccac tgcttgggct ttcggccggc
     3481 tctcgcgacc ggcggccctc ctcttcatcc cctaccttct ctgggtgagt tttgccgcgg
     3541 tcttgaactg ggctctgtgg aggttgaacg catgaggaat cccgagacca ggagccccag
     3601 ggattcgacg gcgtcggctc gaccccagaa ggagccatcc atgacaccac agacaggact
     3661 gcctgggcaa gacagtcgtc ccatccgtgt cgggatcagc agttgcctcc tggggaacga
     3721 ggtccggtac gacggggggc acaagcggga ccggtatctc acggacaccc tgagccgctt
     3781 cttcgagtgg gtgccggtct gtccggaggt ggagatggga ctggggacgc cccgcgagac
     3841 cctccgcctc gtcggcgaac ccgaggcccc ccggctggcc ttcgccaaga ccggggagga
     3901 tcagacgga
//
LOCUS       DBNGZC010000094         5093 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_301543, whole genome shotgun sequence.
ACCESSION   DBNGZC010000094 DBNGZC010000000
VERSION     DBNGZC010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_301543"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..1018)
                     /locus_tag="AC16G0_01810"
     CDS             complement(14..1018)
                     /locus_tag="AC16G0_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098385.1"
                     /translation="MHADYLARRFGVFLLILWLAATLNFFLPRLSGQDPVRVKLLQQA
                     QLGGYVQSGIEAMVKEYETRFGLDKPLWRQYLTYLQDVTRLNFNYSIANYPRTVNEII
                     AEAIPWTIGLLGTTTLLSFGIGTILGALLAWPRAPRWMRWLMPPLWALHAIPFFLFGL
                     ILMYVLAFRFNLLPMFGGYSAGAFPALSLEFAGDVLTHALLPALSIVLVATGGWALAM
                     RGMMVTTQGEDYVTFAEAKGLKSLTIFLRYCVRNAILPQTTALALALGQILSGAVLVE
                     VIFGYPGIGTLLFLAIRENDHFLIQGIVFTVIVALGLATLLLDVIYPLLDPRISYRRK
                     "
     gene            complement(1097..2872)
                     /locus_tag="AC16G0_01815"
     CDS             complement(1097..2872)
                     /locus_tag="AC16G0_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098386.1"
                     /translation="MPERKSAKQLSRREFIKAGSVMAGAAALSPGLAAAQAPRPKAVP
                     RNRTLILTWIGSREGRWVDYDLWNPYSIGSNHQNGPGILYEPLAYYSAFADKEYMWLA
                     ESYKYSPDFKELTIKTRSGIRWSDGAAFSAEDVAYTLNSLRDLGPKVRWGVDVQQFMQ
                     EARATDANTVVVKFKIPSPRFFYFMTYKYDIGVYIVPKHVFQGQDWTTFKHFDVAKGW
                     PVTTSPWKVVFSSPEQKIIDRRDEWWAVKAGLAAMPKVERNIWLPNAGEQQSAQGLIT
                     NQIDYGMSMQPATFPTMIRQNPKIITHSGQKPPYGYMDWWPLSLYVNNERPPFNDKDI
                     RWAVSYFIDRQQIVDVGYVGASIVSPLPLPEYPPLRPYIDSVKDLLTTHNTLEFNPKK
                     GEEILSRKGWKKDSAGFWIDAQGNRVKLDIIGFGGSGPAVGPVITEQLRRNGIDASMS
                     LPPDFDDRFQKGQYTGSIYGHGGSVNDPYHTLRLYQSVSVAVPGGHLVNFARWKNEAY
                     NKIVDDVYVTDMKDKAKLLNLFRKAMEIWIPELPDIPLVHNIHRVPMNTTYWTNWPTE
                     QNPYVNGAFWHLTYAIVLWNLQPTQ"
     gene            3411..4691
                     /gene="lysA"
                     /locus_tag="AC16G0_01820"
     CDS             3411..4691
                     /gene="lysA"
                     /locus_tag="AC16G0_01820"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR5098387.1"
                     /translation="MLDLARYPLERLADRVGTPFYFYAADVLRSSLSQLAELVRAPLL
                     AARYAMKANSARAVLETVRAAGLWIDAVSGNEVVRAVKAGFPGGTDPPVIMLTTDVFR
                     DNALEVVCDHRVLPSLGSPRMVEELAACGYRGPIALRPNPGFGHGHVQACDTGGPSSK
                     HGIWPDDLPTVRRRAESVGMHVVTLNVHVGTGPEPGEFALNMRRVAAFFAETLPEFPE
                     VTAVNFGGGLPHPYHPCDSAYDLATYRPILQDARERLAHVVGHPVRVEIEPGRFLVAG
                     MGVLVTRVKDLKRTRTNDKGVGHTFVMVDAGFCDLLRPALYGAYHQIDVVNTGGVRRG
                     TGPCVVAGPLCESGDVFTRDAGEFLAPRLLPLPEPGDLLVLQDAGAYGASMSSHYVST
                     GQVPQVWWDGGQARLIARRETVEDITCAECDVGL"
     gene            complement(4725..>5093)
                     /locus_tag="AC16G0_01825"
     CDS             complement(4725..>5093)
                     /locus_tag="AC16G0_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR5098388.1"
                     /translation="RFTWEMPTGGVRPGESVEAAAQRELAEEAGRRAGRLTWISTYHT
                     SKSVMNETAHLYLGEDLTPIERAADETEFIERRVFPFHEVLDLVRRGEITDSMTVISV
                     LHAALRPHVEPQADQVSEGR"
BASE COUNT          855 a         1583 c         1642 g         1013 t
ORIGIN      
        1 gccccatgac ggactacttc ctcctgtagc tgatgcgggg atcgagcagg gggtagatca
       61 catccagcag cagcgtcgcc agccccagcg ccacgatgac cgtgaacacg atcccctgga
      121 tgaggaagtg gtcgttctcc cggatggcaa ggaagagtag cgtcccgatg ccgggataac
      181 cgaagatcac ctcgaccagg accgcgccgg agaggatctg tcccagggcc agcgccaggg
      241 cggtggtctg gggaaggatg gcgtttcgga cgcagtagcg caggaaaatg gtgaggctct
      301 tgaggccctt ggcctcggcg aaggtgacgt agtcctcgcc ctgggttgtc accatcattc
      361 cgcgcatggc cagcgcccac ccccctgtgg ccaccaggac gatggacagc gcgggcagaa
      421 gcgcgtgcgt gagcacgtcg cccgcgaact ccaagctcag ggccgggaat gcgccggcgc
      481 tgtagccgcc gaacatcggg aggaggttga accggaacgc caggacgtac atcaggatga
      541 ggccgaagag gaaaaagggg atagcgtgca gggcccacaa cggcggcatg agccagcgca
      601 tccacctggg cgccctgggc caggccagca gcgcccccag gatcgtccca attccgaacg
      661 agaggagggt ggtcgtgccc agcaggccga tggtccaggg aatggcctcg gcgatgatct
      721 cgttcaccgt tcgcgggtag ttggcgatcg aatagttgaa attcagccgg gtgacatcct
      781 ggagatacgt caggtattgc cgccataacg gtttgtccag cccgaaccgc gtttcgtatt
      841 ccttcaccat ggcctcgatg ccgctctgga cgtagcctcc gagttgcgcc tgctggagga
      901 gtttcacccg gacagggtct tgtcccgaca gtcgaggcag aaagaaattg agcgtggctg
      961 caagccatag gatcagtaga aaaacgccga accgcctcgc caggtaatcc gcgtgcacgg
     1021 tctctccact ccgcgcggga aggctgtacc ggtaccgcct ccccgcggct gtgccggcgc
     1081 atacgcgccg ctctggctac tgggtcggct gaaggttcca gagcacgatg gcatacgtca
     1141 ggtgccagaa ggcgccattg acgtatgggt tctgctccgt cggccaattc gtccagtagg
     1201 tggtattcat gggtacgcgg tggatattgt gcaccagcgg gatgtccggc agctcgggga
     1261 tccagatctc catggccttg cggaacaggt tcaggagctt ggccttgtcc ttcatgtcgg
     1321 tgacgtacac gtcgtccacg atcttgttgt aggcctcgtt cttccaccgg gcaaagttga
     1381 ccaggtggcc gcccggcacc gccacactca cgctctgata cagccggagg gtgtggtacg
     1441 gatcattcac gcttccgcca tggccgtaaa tcgatccggt gtactgccct ttctggaacc
     1501 gatcgtcgaa gtccggcggc aggctcatgc tggcgtcgat cccgttgcgc cgcaactgct
     1561 ccgtgatcac gggtcccacc gcgggcccgc tgccgccgaa gccgatgatg tcgagcttga
     1621 cgcgattgcc ctgggcgtcg atccagaagc ccgcgctgtc cttcttccag cccttccggc
     1681 tgaggatctc ctcccccttc ttggggttga actccagcgt attgtgcgtg gtcaagaggt
     1741 cctttaccga gtcgatgtat ggacgcaggg gaggatactc gggcagaggc agcggcgaca
     1801 cgatcgaggc cccgacgtac cccacatcca cgatctgctg ccggtcgatg aagtagctca
     1861 ccgcccagcg gatgtccttg tcgttgaacg gcggccgctc gttgttgaca tagagcgaga
     1921 gcggccacca gtccatgtag ccgtagggag gcttctggcc ggagtgggtg atgatcttgg
     1981 ggttctgccg gatcatggtc gggaaggtgg cgggctgcat ggacatgccg tagtcgatct
     2041 ggttcgtgat cagcccctgg gccgattgct gctcgccggc attggggagc cagatgtttc
     2101 gctccacctt gggcatagcc gcgaggcctg ccttgaccgc ccaccactcg tcccgccgat
     2161 cgatgatttt ctgctcgggg gagctgaaga ccaccttcca ggggctggtc gtgaccggcc
     2221 agcccttggc cacgtcgaag tgcttgaacg tggtccaatc ctggccctga aatacgtgct
     2281 tggggacgat gtagacgccg atgtcgtact tgtaggtcat gaagtagaag aagcgcggcg
     2341 agggaatctt gaacttgacg actaccgtat tggcgtccgt ggcccgcgct tcctgcatga
     2401 actgctgcac atccaccccc cagcggacct tggggcccag gtccctgaga ctattgagtg
     2461 tgtatgccac gtcctccgca ctgaacgccg cgccatcact ccaccggatg ccggatcgcg
     2521 tcttgatcgt caattccttg aaatcgggac tgtacttgta gctctcggcc agccacatgt
     2581 attctttatc ggcgaaggcg ctgtagtagg ccagcggctc gtacaggatg ccgggcccgt
     2641 tctggtggtt ggacccgatg gagtacgggt tccacaggtc gtagtcaacc caccggcctt
     2701 cgcggctgcc gatccacgtc aggatcaacg tccggttgcg gggcaccgcc ttgggccgcg
     2761 gtgcctgggc ggccgccagc ccgggggaca aggcagcggc cccggccatc acgctgccgg
     2821 ctttgatgaa ctctcgtctg ctgagttgct tggcgctctt tctctcaggc atggtattct
     2881 cctttctctg ctagccctgc gcagctgccc ggagaccgtg ttcttccagg cacgtatcgg
     2941 caaatccgcg acaccccgca cgtctgggcg tggccatgtc tggatcctga ggccgacatc
     3001 agttctatcc tgagtgcgat cctagagact ttcttcagat caagcgcctg acggctcgtc
     3061 ctgcttggta ggtaatttgg atactcgagt tactccgggc atggtgggat ggggtgccgg
     3121 aaaactccgg cgggagccta tctctcgcat gctgaggtgt caagggggcc tcgtgcgtat
     3181 ggcctccttt ggacatacga accgactttg ccgcttcggc cgatggacgc catcaaggct
     3241 tcgtttgggc acctctccgc gttgacaaga gcgcacccct tcgctaggct gctgcccacg
     3301 tcttcggatc gcacctctgg gtcgagccct ttctcctgac acggcatcca tggctcgtcc
     3361 tggacagacg atgctcctgg gcgccactcc tcgagccggt aggaggtctg atgctggatc
     3421 tcgcgcgtta tcccctggag aggctggccg accgcgtcgg gaccccgttc tacttttatg
     3481 ctgcggatgt tctgcgctcg agcctgagcc agcttgccga gctcgtccgt gcacccctcc
     3541 tggcggcccg ttacgccatg aaggcgaact ccgctcgggc cgtgctggag actgtccgtg
     3601 cggcgggcct ctggatcgac gcggtcagtg gtaacgaggt ggtgcgcgcg gtgaaggcag
     3661 gcttcccagg tggtacggac ccaccggtga tcatgctgac aaccgacgtc ttccgggaca
     3721 atgcgctcga ggtcgtgtgt gatcaccgtg tgctgccgag tctcggctcc cctcgtatgg
     3781 tcgaggagtt ggccgcatgc gggtaccggg ggccgatcgc gctgcggccc aaccctggct
     3841 ttgggcacgg acatgtccag gcctgcgaca ccggtggccc gtcctcaaaa cacgggatct
     3901 ggccggatga tctgccgacc gtgcgtcggc gcgccgagtc ggtcggtatg catgtggtca
     3961 cgctgaacgt gcacgtgggt accgggccgg agcccggaga gttcgccctc aacatgcggc
     4021 gtgtggcagc cttcttcgcc gaaacactgc ccgagttccc tgaagtcacc gccgtgaact
     4081 tcgggggcgg actcccgcac ccgtaccacc cttgcgattc tgcctacgat cttgcgacct
     4141 atcgcccgat cctgcaggat gcccgtgagc gcctcgccca tgttgtcggg cacccggtcc
     4201 gcgtcgagat agagccgggc cgcttcctgg tggcgggaat gggggtcctg gtcacccgcg
     4261 tgaaggacct caagcgaacg cggacgaacg ataaaggcgt cgggcacacc tttgtcatgg
     4321 tggatgccgg gttctgtgac cttcttcggc cggcattgta cggggcctac catcagattg
     4381 acgtggtgaa caccggcgga gtacggagag gaaccggacc gtgcgtcgtg gcggggccgt
     4441 tgtgcgagag cggcgacgtg ttcacgcgcg atgccggcga gttcttggct ccgcgcctgc
     4501 tcccgctgcc cgagccaggc gatctcctgg tcctgcagga tgcgggggcg tatggcgcgt
     4561 ccatgagctc acactacgtc tccacgggac aggtgccgca ggtatggtgg gatggcggac
     4621 aggctcgcct gatcgcacgc cgggaaacag tggaggatat cacgtgcgcc gaatgcgacg
     4681 tgggccttta gcaggctgct gaataggatc gcaccccgct acccctatcg tccctcagat
     4741 acctgatctg cctgtggctc cacgtgcggt ctcagcgcag cgtggagcac cgagatcacg
     4801 gtcatgctat cggtgatctc gccccgccgg accagatcca gaacctcatg gaaggggaag
     4861 actcgccgct cgatgaactc ggtctcatcg gccgctcgct ctatcggcgt gaggtcttcg
     4921 ccaaggtaga ggtgggctgt ctcattcatc acgctcttgc tggtatggta ggtgctgatc
     4981 cacgtcaggc gtccggctcg gcgcccagcc tcttcggcca gttcccgttg cgctgccgcc
     5041 tccacggatt ccccggggcg gacgccgcct gtgggcatct cccacgtgaa ccg
//
LOCUS       DBNGZC010000095         2098 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2355850, whole genome shotgun sequence.
ACCESSION   DBNGZC010000095 DBNGZC010000000
VERSION     DBNGZC010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2355850"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            535..>2098
                     /locus_tag="AC16G0_01830"
     CDS             535..>2098
                     /locus_tag="AC16G0_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098389.1"
                     /translation="MDVGDSSSIRTTALLQHLNSLKTVSVPDPPDYAVYVRDRNALRL
                     LGKALFWDQQLGSDGQACASCHFHAGADNRSLNQLDPGLRNTTPGVDNTRFSTELGFG
                     PNYQLQMTDFPFHKLSNPDDRTSALISDTPNVTSSQGVFNLNFTALGIPGDTGVPNLT
                     GPGAIFNVGGSVLVRNVAPRNTPSVLNSVLNHRNFWDGRARSEFNASNPIGLLDPTAQ
                     IVQVGEGGQANFVSVRIENSSSASQAVGPALSNMEMSFNGRQFPQLGRKMLSDSLVPL
                     GQQFVAPDDSLLGLYSRYPERGIRFGYEKLVRKAFQRAWWDAPGQVVDVSGATPVIKQ
                     GGPGSHQFSIMEYNFPLYFGLAIREYEKLLISNDSPFDRFMEGDLSALSAEEQQGLVV
                     FMGRGKCIACHGGPEFTNAGVTNFERLQVLERMIVGNFQTVQDLNEGYPRDPIGFHLA
                     VYDNGFYNIGVRPTTEDLGVGDRIGPDNLPLSNSRRFLEQVQSRVKALQAADPNLDLD
                     SVVLQANQEFGVP"
BASE COUNT          360 a          716 c          655 g          367 t
ORIGIN      
        1 ggcatcagcc aggtgcaggg cctggcgacg cgcccgttcc ccttgcagct ctcccagccc
       61 gccgtaagct ctccggtccg gcaaccgcag ccggcggctc tgtccggtgc tcggagcgga
      121 ggcgggcaag taccgcagag ccaggggggc ggcgtgaccg ttgccccggc gcctcccccc
      181 gctgctccca cttcaccgtc cgcgccggct gatccgtccc ccacggcttg ggtcagccaa
      241 ctgctgctcc gggcgcgaaa gcaccgcctg cgcccgggac ggagccgcgg aacgctgttg
      301 ctggaggaaa tcggccccga cccccttccc cacgcgcaag accatggtgc tcgcgatcgg
      361 cgcgccgtgg ctggcggctg taatgcctcg gaccgctcct ccggctccct ggatccatgc
      421 cgtgggttcg gcccgcagga tcgccggcct gacaccccca tcctgtcggg gctcgactcg
      481 ctggccttat cgacctcggc attcgcttca acgctgacga atccctccgt catcgtggac
      541 gtcggggact cctccagcat ccggacgacg gcgctcttgc agcacctgaa ctcactcaaa
      601 acagtcagcg tgcctgaccc tccggattat gcagtgtacg tccgcgatcg gaacgccctg
      661 cgactcctcg ggaaggcact cttctgggat cagcaactgg ggagcgacgg ccaagcctgc
      721 gccagctgtc acttccacgc gggggcggac aaccgatccc tcaaccagtt ggacccgggt
      781 ctccgaaaca cgactccggg cgtggacaac acccgcttct ccaccgagtt gggctttggt
      841 ccgaactacc agttgcagat gacggacttt cctttccaca agctgagcaa cccggatgac
      901 cgcacctcgg ccctgatctc cgacacgccc aacgtcactt cgtcgcaagg cgtcttcaac
      961 ctgaacttca ccgcgctcgg gatcccgggc gacaccggcg tgccgaacct caccggaccg
     1021 ggcgccatct tcaatgtggg cggatccgtg ctcgtgcgaa atgtggcgcc tcgcaacacg
     1081 ccttccgtgt tgaactccgt cctgaaccac cggaacttct gggatgggcg ggcccgcagc
     1141 gagttcaacg ccagcaatcc gatcggcctc ctcgacccga cagcccagat cgtacaggtc
     1201 ggcgagggag gtcaggcgaa cttcgtgagc gtccggatcg agaattcgtc cagcgcatcg
     1261 caggcggttg ggcctgcgct cagcaacatg gagatgtcct tcaacggccg gcaattcccg
     1321 cagctcggtc ggaagatgct gtcggacagc ctcgtgcccc tgggtcagca attcgtggcg
     1381 ccggacgaca gcctgctggg gctttacagc cggtatcctg agcgtgggat ccggtttggg
     1441 tacgagaagc tcgttcgcaa ggccttccag cgggcgtggt gggacgcgcc ggggcaggtg
     1501 gtggacgtct ccggagcaac gccggtgatc aagcagggcg gtccgggatc gcatcagttc
     1561 agcatcatgg aatacaattt ccccctgtac ttcggcctgg cgatccggga gtacgagaag
     1621 ctcttgatct ccaacgactc ccccttcgac cgcttcatgg agggggacct cagtgcgctg
     1681 agcgcagagg agcagcaggg cctggtggtc ttcatggggc ggggcaagtg catcgcctgc
     1741 cacggcggac ccgagttcac gaatgccggc gtgacgaact tcgagcggct ccaggtgctt
     1801 gagcgcatga tcgtgggcaa cttccagacg gtgcaggacc tcaacgaggg atatccgcgg
     1861 gatcctatcg ggttccacct cgctgtctat gacaacggct tctacaacat cggtgtccgt
     1921 ccgacgaccg aggacctggg tgtgggtgac cggatcgggc cggacaacct cccgctctcc
     1981 aacagccgcc gtttcctcga gcaggtgcag agtcgagtga aggccttgca ggcagcggat
     2041 ccgaacctgg atctcgacag cgtggtcctg caggccaatc aagaattcgg tgtgcccc
//
LOCUS       DBNGZC010000096         3790 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2454060, whole genome shotgun sequence.
ACCESSION   DBNGZC010000096 DBNGZC010000000
VERSION     DBNGZC010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2454060"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..1243)
                     /locus_tag="AC16G0_01835"
     CDS             complement(203..1243)
                     /locus_tag="AC16G0_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR5098390.1"
                     /translation="MSESRRYTRWMHDQLSRREFLQAGSGALGGLMLAGWGRPAGAAA
                     ASDVAGKTIGFSMSWTTTEWVVQQHQGVTQMAAKYGMKVISFDAGDNPAKEVTNLEDL
                     VTRKVDVIIISTYYAEAITPAIRTINSAGIPLVVLSSSLATGTNFNSHLSVDTLATAR
                     EAGRLYVKRLGGKGKVVQIEGKPGSVVNQMRGKGFREVIDKEKGIEVVAHVVANYERG
                     QALRVMEDVLRSQRKIDAVYCHNNEMALGAIRAAKEAGRAKEMWFTGYDGLTVEELQA
                     IYNGDLYGCFYYLPFGAEGVEVAVRILRGQKVPKEIVYPSPFMSKENITDWYDPATKS
                     RKPAVPRPIPEA"
     gene            complement(1351..2367)
                     /locus_tag="AC16G0_01840"
     CDS             complement(1351..2367)
                     /locus_tag="AC16G0_01840"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098391.1"
                     /translation="MPNTPSPMSAGGSTAVDDAPRSMVRAFSAGGLGRLLIRWSNIWA
                     LALLFGISAAISPYFLSVTNIFNVIRGASMVGIVSIGMTFVILNRGIDLSVGSILGLS
                     AALAAGMASHGSAFVLILALLVGSLLGLINGWIITRLQLQPFIATLGMLIFARGVVYI
                     YTSGSIIVVQNPPESFTYLGSGRIGPVPVPIVLFVLVWAIAAYVLSRTVYGREVYAVG
                     ANEEGARLSGINVMRNKVLVYGISGFLASLAGVVLLSRLTAAEPNAGTLFELDAIAAT
                     LIGGTAFEGGIGGVHGTIAGVLILAFLGNILNLTNVSPYTQMLLKGVIIVLAVVVSEL
                     KNRR"
     gene            complement(2360..>3790)
                     /locus_tag="AC16G0_01845"
     CDS             complement(2360..>3790)
                     /locus_tag="AC16G0_01845"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR5098392.1"
                     /translation="LALRAGEIHALVGENGAGKSTLMRIFDGIYQPDTGEIRIDGRVV
                     QIDSPHRAILLGVSMVHQEPKLALPLSIAENIFMGRLPTKGQGRVDWPRLNANARELL
                     GSLGLDLDPRTPAIGLSIAHRQLVQIAKALSYRTRVIILDEPSASITPVELETLFGVL
                     LRLKERGVGLIYISHRLDEVFRIADTVTVLKDGRRVAAMPVAQIDKSGLIALMVGRKL
                     GYTFPPKRQRAGEIVLEVRGFSGEGFRNISFTARRGEILGIAGLVGAGRTEMIRAIFG
                     AELPAHGELSLHGRRVRVSSPRDAIRLGIGYLAEDRRDSLIMPLSVRENITVATPEKV
                     SRYGLFDGVRQRAIAQEYVAKLNVRTPTIEQLVLNLSGGNQQKVAFAKWLVKGTEVLI
                     FDEPTRGIDVGAKVEIYLLLDQLAREGKAIIMISSELVEILGMSDRVLVISEGRLVGE
                     LPGEGATEERILELAIPHSTRESSHA"
BASE COUNT          699 a         1243 c         1136 g          712 t
ORIGIN      
        1 gcctctgttt tcgggagaat aattggtcgg ggggactggg gttgagccag cggccacttg
       61 gtcccgaata ctctcccgca cgctttcgca cagacgtggg ctctgcgccg attaggattt
      121 cgagcggcat cccttcccag gcatcgggga cacacggcgc cggtccgcca ccccgctccg
      181 agcttccctc ggcgcggtcg cctcaggcct cgggaatggg tcgaggtacc gcaggctttc
      241 ggctctttgt ggccggatca taccagtcgg tgatgttctc cttgctcatg aatggggatg
      301 gatacacgat ctccttgggc accttctgcc cacgcaggat gcgcacggcg acctccacac
      361 cctcggcgcc aaacggcagg tagtagaagc agccatacag gtccccgttg tagatggcct
      421 gcaactcttc cacggtcaga ccgtcatagc cggtgaacca catctctttg gcgcgtcccg
      481 cttccttcgc ggcgcggatc gcgcccaggg ccatctcgtt gttgtgacag tatacagcgt
      541 cgatcttccg ctgggaccga aggacgtctt ccatgacgcg caaggcctgt ccgcgctcgt
      601 agttggcgac cacgtgggcg accacctcga tgcccttctc cttgtcgatg acctcacgga
      661 aacccttgcc gcgcatctga ttgaccacgg agccgggctt gccttcgatc tgcacgacct
      721 ttccttttcc accgagccgc ttcacgtaga gtcttccggc ctccctggcg gtcgccagcg
      781 tgtccacgga aaggtggctg ttgaaattcg tccccgtcgc cagactggag ctgagcacca
      841 ccagagggat gccggcgcta ttgatggtgc ggatggcggg cgtgatggct tcggcgtagt
      901 aggtcgagat gatgatcacg tcgacttttc gggtgaccaa gtcttcaagg ttggtgacct
      961 ccttggcggg attatccccg gcgtcgaagg agatcacttt catgccgtac ttggcggcca
     1021 tctgagtcac accctggtgc tgctgcacga cccactcggt ggtggtccag gacatggaga
     1081 agccaatggt cttgccggcg acatcgctgg cggcggccgc tccggcgggc ctcccccagc
     1141 cggccaacat caaaccgcca agcgcacctg accccgcctg aagaaactca cgccggctga
     1201 gctggtcgtg catccaccgt gtatatcgcc tggactcgga catggctatc ctccttcctc
     1261 atggagcagc aatcagggtg tcccctccga gcgtgattgc tcttacggtc cttccgctgc
     1321 accacggatc cccagtgggg agagcagttc tcatcggcga ttcttcaatt cgctcacgac
     1381 cacggccaag acgatgatca cccccttgag cagcatttgg gtgtacggtg agacgttcgt
     1441 caaattgagg atgtttccca ggaaggccag gatcaagacc ccggcgatgg tgccatgcac
     1501 gccaccgatc ccgccttcga acgccgtgcc gccgatcagc gtggctgcga tggcatccag
     1561 ttcgaagagg gtgcccgcat tgggctccgc cgcggtcagc cgactcagca gcaccacacc
     1621 ggccagcgac gccaggaatc cgctgatacc atagaccagg accttgttgc gcatcacgtt
     1681 aatgccggag aggcgtgcgc cctcttcatt ggccccgacg gcatagacct cacgaccata
     1741 cacggtccgg ctcaacacgt aggcggcgat cgcccacacc aacacgaaga gaacgatggg
     1801 aaccggcact ggcccgattc ggccggagcc caggtaggtg aagctctcgg gcggattctg
     1861 cacgacgatg atgctgccgc tggtgtagat gtatacaacg cctctcgcaa agatcagcat
     1921 gcccagcgtg gcaatgaagg gttgcagctg gagccgggtg atgatccacc cgttgatgag
     1981 acccagcagg gatcccacca agagcgccag gatcagaacg aaggcggagc cgtgtgaggc
     2041 catgccggcc gccagggcgg ccgagaggcc gaggatggag ccaacggaga ggtcgatccc
     2101 ccgattcagt atgacaaacg tcatcccgat gctgacgatg ccgaccatgc tggcacctcg
     2161 gatcacgttg aagatgttcg tgacgctcag gaagtaggga gagatggccg cggaaatgcc
     2221 gaacaagagg gccagcgccc agatattgct ccaccggatg agcaggcggc cgaggccgcc
     2281 cgctgagaac gcccggacca ttgaccgggg ggcgtcatcc acggcggtgg agccacctgc
     2341 ggacattggg gaaggagtat taggcatgac tgctctccct cgttgaatgc ggaatcgcca
     2401 gctcaagaat ccgctcctcg gtcgcgcctt cgcccggcag ttctcccacc aaccgaccct
     2461 cgctgattac gagcacccga tcgctcatcc ccaggatctc gaccagctcg gacgagatca
     2521 tgatgatggc tttgccttct cgagcgagtt gatccagcag gagatagatc tccaccttgg
     2581 ccccgacgtc gatcccccgg gtcggctcgt cgaagatgag aacctcggtc cccttcacca
     2641 gccatttggc gaacgccact ttctgctggt ttcccccgct caggttcagg accagttgct
     2701 cgattgtggg ggtccgcacg ttgagcttgg ccacgtactc ttgggcgatg gcacgctggc
     2761 ggacgccgtc aaagagtccg tagcgactca ccttctcggg ggtggccacg gtgatgttct
     2821 cccgcacgct caacggcatg atcaggctgt cccggcgatc ctcggccagg tagccaatcc
     2881 ccagccggat cgcatccctc ggcgacgaga cgcgcacccg ccgcccatgc aggctcagct
     2941 ccccatgcgc cggtagctcg gcaccgaaaa tggcccggat catttcggtc ctcccggccc
     3001 ccaccagtcc cgcgatcccc aggatttctc cgcgccgcgc ggtgaagctg atattcctga
     3061 agccctcccc gctgaagcca cgcacctcca gaactatctc acccgcccgc tggcgcttgg
     3121 gtggaaaggt gtagcccagc ttcctgccca ccatgagcgc gatgagtccc gacttgtcga
     3181 tctgtgccac gggcatggcg gcgacgcggc gaccatcctt caacacggtc acggtgtcgg
     3241 cgatccggaa cacctcatcc agccggtggg agatgtagat gaggccgacg ccccgctcct
     3301 tgagcctcag caggaccccg aagagggttt ccagctccac gggcgtgatg ctggcagacg
     3361 gttcgtcgag gatgatgact cgcgtccgat aggagagcgc cttggcgatc tgcaccagtt
     3421 gccgatgcgc gatgctcaag ccgatagctg gcgttcgcgg atccaggtcc agaccgagac
     3481 tccccagcaa ctcgcgagcg ttggcattca gccggggcca atcgacccgc ccctgccctt
     3541 tggtcggcag gcgtcccatg aagatgttct cggcgatgct gagcggcagg gcgagcttcg
     3601 gctcctgatg gaccatgctg acgccaagca gaatggcccg atgcgggctg tcgatctgca
     3661 ccacacggcc gtcgatgcgg atctctccgg tgtcgggctg atagatgcca tcgaagatcc
     3721 gcatcagcgt cgatttgccc gcgccgtttt cacccacgag cgcatggatt tccccggctc
     3781 tcagggccaa
//
LOCUS       DBNGZC010000097         2119 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_986652, whole genome shotgun sequence.
ACCESSION   DBNGZC010000097 DBNGZC010000000
VERSION     DBNGZC010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_986652"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..721)
                     /gene="purN"
                     /locus_tag="AC16G0_01850"
     CDS             complement(116..721)
                     /gene="purN"
                     /locus_tag="AC16G0_01850"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010077288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR5098393.1"
                     /translation="MLTLHLGFLASHGGSNMQAILDACKGGRLRAKPCVVISNNSDSM
                     ALQRAETGGVPGYHISSQTHPGEAEDREILRVLRLHGVDTVILAGYMKRLGPVTLAAY
                     RGRILNIHPALLPKFGGKGMYGRHVHEAVLAAGEQVTGVTVHVIDENYDTGPILNQCR
                     VPVQPGDSVETLSARVLKQEHRLYVETLQKISEGEIILCGA"
     gene            complement(979..1371)
                     /locus_tag="AC16G0_01855"
     CDS             complement(979..1371)
                     /locus_tag="AC16G0_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR5098394.1"
                     /translation="MATIQPYCAKDVFDPPGYTQAIKVTGAQTILFLSGQVAYDEKGG
                     PAHRGDFTAQARTVFRAIKAQVEAGGGTLANVVKLTTYLTDIRHRADLVPVREEFFGK
                     KLPASTLIGVAALAHPDWLIEVEAIAVV"
     gene            complement(1428..1925)
                     /locus_tag="AC16G0_01860"
     CDS             complement(1428..1925)
                     /locus_tag="AC16G0_01860"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HIR5098395.1"
                     /translation="MLSKAVQNAINEQINAELYSAYLYLSMSAYCAATNRPGSAGGMR
                     VQAQEELGHAMKFFGYVTDRGGRALLKAVGQPPAEFKSLLDIFQQALEHEEEVTRKIH
                     KLYEIATKENDYATQVELQWFITEQVEEEKTAGEIVQQLKMIGDQAAPLFMLDRHLGM
                     RAGGK"
BASE COUNT          308 a          735 c          658 g          418 t
ORIGIN      
        1 cgccctcccc gcgcacccac gcctgcggcg cgggtacccg ccgagagagg tgggccgcca
       61 cccctcctcg gaccctcccc cggcctgtgc ggtcttggtg ggagtgtcgc ctagtctacg
      121 caccgcacag gatgatctcc ccctcgctga ttttctgtaa ggtctcgacg tagagccggt
      181 gctcctgctt caagaccctg gcgctcagcg tctccacgct atcgcccggt tgcaccggca
      241 cccggcactg gttcaggatc ggcccggtgt cgtagttctc gtcgatcacg tggacggtga
      301 ccccggtgac ctgctccccc gccgccagga ccgcctcgtg gacgtgcctc ccgtacatcc
      361 ccttgccgcc gaacttcggc aggagcgcgg ggtggatatt caggatcctc cctcgatacg
      421 ccgccagggt gaccgggccc agccgcttca tgtagccggc caggatcacc gtatccaccc
      481 catgcaggcg caggacgcgc aggatttcgc ggtcctctgc ctcgcccgga tgcgtctgcg
      541 agctgatgtg atacccgggg acgcccccgg tctcggcccg ttgaagggcc atggagtccg
      601 agttgttgct gatgaccacg cacggcttgg cgcggaggcg cccccccttg caggcgtcaa
      661 ggatcgcctg catgttgctg ccgccgtgcg acgccagaaa gcccaggtgc agggtcagca
      721 tctgctcgcc catctcaccg caggtcccgc cccatgcaag acacgacggc ccctcaaggc
      781 ttgtgtcgcc tcaaggggcc gtcggaattc cggtccaatt cgccggtctg tgtcaagctg
      841 gattcatcgc ttccggtggc cgccatcatc caggtccctc gccgcaatgg acgctgccag
      901 gcgctccgga tagaggctag tcccattccg ttgccgatgt caatccctcc ctgtgtgccc
      961 gccgtcctta cccgagcatc agacgaccgc gatggcctct acctcgatca gccaatccgg
     1021 atgggccagg gcggccaccc cgatcagcgt ggacgcgggg agcttcttgc cgaagaattc
     1081 ctctcgcacc ggcaccaggt cggcgcggtg ccggatgtcg gtgagataag tggtcagctt
     1141 cacgacattc gccagggtac ctcccccggc ttccacttgg gccttgatgg cccggaagac
     1201 ggttcgagcc tgggccgtga aatccccccg gtgggctggt ccccccttct cgtcgtaggc
     1261 cacctggccc gagagaaaga ggatggtctg ggctccggtg accttgatgg cctgggtata
     1321 gccgggtgga tcgaagacgt ccttggcgca gtacggctgg atcgtcgcca tcgctgcctc
     1381 cttcccgccc cgtgccccga gaggtgcctc gggtgtgggc agtgcgatta cttcccgccc
     1441 gcccgcatgc ccaggtgccg gtccagcatg aagagcggtg cggcctgatc gccgatcatc
     1501 ttcaactgct ggacgatctc acccgcggtt ttctcctcct ccacctgctc cgtgatgaac
     1561 cattgcagct cgacttgggt ggcgtagtcg ttctccttgg tcgcgatctc gtagagcttg
     1621 tggatcttgc gtgtcacctc ttcctcgtgc tccagagcct gctggaagat gtccagcagg
     1681 gacttgaact ccgcgggcgg ctggccgacc gccttgagca gcgcccgccc gccgcgatcg
     1741 gtcacgtagc cgaagaactt catggcgtgc ccgagctcct cctgggcctg gacgcgcatc
     1801 cctcccgcgg agcccggccg atttgtggcg gcgcagtagg cggacatcga caggtacagg
     1861 taggcggaat agagctctgc gttgatttgc tcgttgatag cgttttgaac ggccttgctc
     1921 agcatggtcg ccctcctctc tgtggtgtgt ggtcctgcgt ctcacctcga ccgggattcc
     1981 tgcttatgca ggctcggaca gacgcaggat gatgtcggcg gcggtccggg cggcgatccc
     2041 gcagatctgc ggacagccgg tgcgatcgtg gccccctgcc tcgtgaaaag ccttgacctc
     2101 cgcgggatcg tacagacgg
//
LOCUS       DBNGZC010000098         4723 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2649129, whole genome shotgun sequence.
ACCESSION   DBNGZC010000098 DBNGZC010000000
VERSION     DBNGZC010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2649129"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="AC16G0_01865"
     CDS             <1..315
                     /locus_tag="AC16G0_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098396.1"
                     /translation="FAVGNAVTGKGNILISQKHGRTVSQNMLEHYLLGTASGYEEILA
                     GAAVSAQEKVEAIAERLAGRAPLPAPRVASLLSWVKALQGRVSYPGEYRTWIELVRLP
                     EV"
     gene            613..1089
                     /locus_tag="AC16G0_01870"
     CDS             613..1089
                     /locus_tag="AC16G0_01870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR5098397.1"
                     /translation="MRSLAAAVTVVTTRDREGEPRGLTATAVCLVSFEPQLLLVCIDR
                     TAECHEAFLTAETFAVHLLREGQEALSRRFARKDSQKFEGISHRQGVMGVPILADVLA
                     SVECRVHARYPGGDHTILVGEAAHSVVAPSGEAGTPLVYFQGCYAHLGNRLPREAP"
     gene            1189..2697
                     /locus_tag="AC16G0_01875"
     CDS             1189..2697
                     /locus_tag="AC16G0_01875"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5098398.1"
                     /translation="MWPKGGWNLSLRGKLLAMMFGLLMLVLAILFLMYWRAESNLIAQ
                     VEHHTTDLSTAIQISVEQLTSKGRTSEARLQDYVQRLQRRGVREISIVSNEQEVIASS
                     NPRRVGVRVDPKRKDVLITARLGEESAGGLNQKTYNLFVPVVVSNQRSGYILISMILD
                     DFSELVRVNFLKRLIATILVFAVGIAAAVALAWTYTKPISRLVEAARRVAQGHLDERL
                     PETRRDEIGRLNRSFNEMVEGLRGKVELEARLHQAERMSAMAHLASGIAHEVRNPLNL
                     INLSIDHLRARFFPAAPAEQEEFGRVVDHIKQEVHRLNNMIETFLKYGKPLKLDRRPA
                     DVRTLLDEMLEVARQRAASQQVQVAREYAATMPEVWVDAPHLKTCFLNLILNAFQAMP
                     EGGVLTVAAGALTGPEGQGPGLGPPALSPQSPHSNGWVEVRIQDTGIGIAPEDLSRAF
                     EPYFTTKEVGIGLGLALTKKILEEHGGHIELTSDPGKGTVARLRLPAGVPST"
     gene            2694..4145
                     /locus_tag="AC16G0_01880"
     CDS             2694..4145
                     /locus_tag="AC16G0_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR5098399.1"
                     /translation="MSKGQILVVDDEASQREILRAILSSEGYRVEIAGSVAEAIQKSE
                     QKRYDLVLTDLRMPGADGLSLVGRLTREDPPTLVILMTAYGSIDTAEQALRQGAFDYL
                     TKPLEREELLLIVSRAFERIRLIQENRLLRQQLEERFRVEGIVGSHYRMQEVFDKLHR
                     ISSSTSTVLITGESGTGKELIARSIHRHSPRKDRPFVAISCAAIPETLIESELFGYEK
                     GAFTGAVSRRQGLFEAADKSTLFLDEVGELNVNLQAKVLRALQEREIRRVGGREDLKV
                     DVRIIAATNKDLEAEVARGTFREDLFYRLNVVTINLPPLRERSTDLPQLVEYFVARAC
                     TEAGRPPMAITTEAMRLLMSYHWPGNVRQLEAVLQRAVLLSGGQTIDYMDLPIEVRFS
                     SLPPREPQETVASGGGYRFALPSQGINLEEVEREFILQAMEISGGVIAKAAKLLGLSY
                     RTLQYRLEKFQIRRDGGEATGSPEEQEAGKAEP"
     gene            4191..4286
                     /locus_tag="AC16G0_01885"
     CDS             4191..4286
                     /locus_tag="AC16G0_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_369335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein bS22"
                     /protein_id="HIR5098400.1"
                     /translation="MGSVVKKRRKKIAKHKYKKLLKRTRHQRRNK"
BASE COUNT          885 a         1418 c         1618 g          802 t
ORIGIN      
        1 ttcgccgtcg ggaatgcggt gactggcaag gggaacatct tgatctcgca gaagcacggg
       61 cgcacggtat cccagaacat gctggaacat tacctcctgg gaaccgccag tgggtacgag
      121 gagatcctgg cgggtgcggc ggtatcggcg caagagaagg tggaggcgat agccgaacga
      181 ctggcgggtc gggctcccct gcccgccccg cgggtcgcga gcctcctgtc ctgggtgaag
      241 gcgctccagg gtcgtgtgtc gtacccgggg gaataccgca cgtggatcga gctggtacgt
      301 ctgccggagg tgtgaggagc cgggattgta gggcatgagg cacaaggcgt gaacccggat
      361 gacgccaggt gacgcgtcgc gaattggcga agccggggag ttggtgggtg ggcacgggaa
      421 gatcccggag gcccccatcc gcgggactcc gacccccgaa acccctcttt cgcctcagcc
      481 tcgagcggtc cgcaggcctt gctgtttctg cctaacacgt gtatcctccc agccgcatcc
      541 cgcggggacg ggctcttcga ccgccggggg aaagggccga tgcttatgga ggtgaagagt
      601 ttccgcaggt tgatgcgatc gctggcggcg gccgtgaccg tggtgaccac gcgggaccga
      661 gagggggagc cgcgggggct cacggctacc gcagtttgcc tggtctcctt cgagccacag
      721 ctcctgttgg tttgcatcga tcgcacggcc gaatgtcacg aagcattcct cacggccgag
      781 acctttgcgg tgcacctgct gcgggaaggc caggaggccc tgtcacgacg tttcgccagg
      841 aaggattcgc agaagttcga ggggatctcg caccggcagg gggtcatggg agtgcccatc
      901 ctggcagacg tgcttgcctc cgtggagtgt cgggtgcacg cgcggtaccc tggcggggat
      961 cacacgattc ttgtgggaga ggccgcgcac tctgtcgtcg ccccgtccgg cgaggcaggg
     1021 actcccttgg tgtacttcca gggatgttac gcccacctgg gcaatcgact cccccgcgag
     1081 gccccctagg cacggttcgt gcctgcccgt tcctgcgccc cgcactgttt catcctttac
     1141 agccagcgtc cgtcttggat agactacccg gaaagggagg ccgaaccgat gtggcccaag
     1201 ggtgggtgga atctcagtct acgaggcaag ctgctcgcaa tgatgttcgg gctgctgatg
     1261 cttgtgctgg ccatcctctt tctcatgtac tggcgggcgg agagtaacct catcgcccag
     1321 gtggagcatc acaccacgga tctcagcacg gccatccaga tcagcgtgga gcagctcacc
     1381 tccaaagggc ggaccagcga ggcccgcctc caggattacg tgcagcggct tcagcggcgt
     1441 ggcgtgcgcg agatatccat tgtcagcaat gagcaggaag tcattgcgtc ctccaatccc
     1501 cgccgggtcg gcgtccgcgt ggacccgaag cggaaggatg tgctcatcac ggctcgcctc
     1561 ggtgaggaga gcgcgggcgg tctgaaccag aagacctaca acctgttcgt tcccgtggtg
     1621 gtgagcaacc agcggagcgg ctacatcctg atcagcatga tcctggatga tttttcagaa
     1681 ctggtccgcg tcaatttcct caagcggctc atcgccacca tcttggtttt cgcggtgggc
     1741 attgcggccg ccgtggccct ggcctggacc tacaccaagc ccatctcacg tctggtggag
     1801 gcggcgcggc gagtggccca gggccacctg gacgaacgtc tccccgaaac ccgccgggat
     1861 gagatcggca ggttgaaccg cagcttcaac gagatggtcg agggcctgag ggggaaggtg
     1921 gaattggagg cgcgcctgca ccaagcggag cgtatgtcgg ccatggcaca cctggcgtcg
     1981 gggatcgctc acgaggtgcg gaatcccctg aacctgatca atctgagcat cgaccacctt
     2041 cgggcacggt ttttcccagc ggccccggcc gagcaggagg agttcggtcg cgtggtggac
     2101 cacatcaagc aagaggtcca ccgcctgaat aacatgatcg agacgttctt gaagtacggc
     2161 aagcctctca aactggaccg gcggcccgcc gacgtgcgga cgctcctgga cgagatgctg
     2221 gaggtggccc ggcagcgggc ggcctcacag caggtgcaag tggcacggga atacgccgcg
     2281 accatgccgg aggtctgggt ggatgctccg catctcaaga cctgcttcct gaacctgatc
     2341 ctcaatgcgt ttcaggcgat gcccgagggc ggtgtgctga cggtggcagc gggcgcattg
     2401 acgggacccg aaggccaggg accagggcta ggaccaccag ccctcagccc acaatccccg
     2461 cattcgaatg gatgggtgga ggtgcggatc caggatacgg gaatcgggat tgcccccgag
     2521 gacctctcgc gtgccttcga gccctacttc acaaccaagg aggtggggat cggtctgggt
     2581 ctggccctga caaagaagat cctggaagag cacgggggcc atattgagtt gactagcgac
     2641 ccggggaagg ggacggttgc ccggctgcgc ttgcccgcgg gagtgcccag cacatgagca
     2701 agggtcagat cctggtggtg gacgacgaag cctcgcagcg cgagatcctg cgggccatac
     2761 tctcctctga aggctaccgg gtggagatag ccgggagcgt tgccgaagcg attcagaaga
     2821 gcgaacagaa gcggtacgat ctggtgctta cagaccttcg gatgcccggg gccgacggcc
     2881 tctccttggt ggggcggctc acgcgtgagg atccaccgac gctggtgatt ctcatgaccg
     2941 cctacggctc catcgatacg gccgagcagg ccttgcgcca aggggcgttt gactacctga
     3001 ccaagccgct ggagcgagag gagctgttgc tcatcgtcag ccgggccttc gagcggatcc
     3061 ggctcatcca ggagaaccgg ttgctccggc agcaactcga ggagcgcttc agggtcgaag
     3121 ggatcgtcgg cagccactac cgcatgcagg aggtcttcga caagcttcac agaatttcga
     3181 gcagcacttc caccgtgctc attaccgggg agagcggcac cggcaaggaa ttgatcgccc
     3241 ggtccatcca ccgccatagc ccccgaaagg accggccatt cgtggcgatc agttgcgccg
     3301 ccattcctga gacgctgatc gagagcgagt tattcgggta cgagaagggg gccttcaccg
     3361 gggccgtcag tcgccgacag ggcctcttcg aggcggcgga caagagcact ctgttcctgg
     3421 acgaggttgg cgagctgaat gtgaatctcc aggcgaaggt cctccgagcg ctgcaggagc
     3481 gggagatccg ccgggtgggc ggacgcgagg atctgaaggt ggatgtccgg atcatcgccg
     3541 ccaccaacaa ggacctggaa gccgaggtgg cgcgtgggac ttttcgggag gacctgttct
     3601 accgcttgaa cgtggtgacc atcaacctgc cgcccctgcg ggagcggagc acggatctcc
     3661 cccagcttgt cgagtacttc gtcgcgcggg cctgcaccga ggcggggcgc ccccccatgg
     3721 ccatcacgac tgaagccatg cgcctcctca tgagctacca ctggccgggc aacgtccgcc
     3781 agctcgaggc ggtgctgcaa cgcgcggttc tgctctccgg cggccaaacg atcgactaca
     3841 tggatctgcc catcgaggtc cgattctcca gcctgccgcc gcgcgagccg caggaaaccg
     3901 tcgcctcggg aggcgggtac cgcttcgccc tgccgtccca ggggatcaat ttggaagagg
     3961 tggagcggga attcatcctg caggccatgg aaatctccgg gggggtcata gccaaggcgg
     4021 cgaagctctt gggcttgagc tatcggacgc tgcagtaccg gctggaaaag ttccagattc
     4081 gccgagatgg gggtgaggcg acagggagtc cggaagagca agaggccggg aaggccgagc
     4141 cttaaccgtc aaacacgcag gccgccctgc ggcgcgaaag gggggatggc atgggtagcg
     4201 tcgtcaagaa gcgtcggaag aagattgcga agcacaagta caagaagctg ctgaagcgga
     4261 cccggcacca gcgccggaac aagtaagtac gctatcttgc ctgggtgggg gccggacgtc
     4321 agagggagaa gcctcgggcg gacggccccc gtgccacgcg gggaccggtt gcccaatagg
     4381 ccgttcgaac ttctcagagc ccccgggttt gctgatcgag actaaaccgc tggcgggcta
     4441 tctggtctaa ctccatagaa gaaggaagga ggccaggcaa gggcgcaggg gcacggcctg
     4501 tcgggccctg agtttcggca ggcgtatccg ctgaggcggc ccccgcgcct cggcgtgcgg
     4561 ggccgaggtt ttcggaaccc tcgaatgctc caagggcagc gccaggagga ttcatttcca
     4621 cgcggtccag aggctgcagc ggaagctcca ggtgttcccc agcgctggct cgtccaatct
     4681 cgaacgggga gaccgtcgtg ctggcgaatc gccggacgca ttt
//
LOCUS       DBNGZC010000099         2031 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_8844, whole genome shotgun sequence.
ACCESSION   DBNGZC010000099 DBNGZC010000000
VERSION     DBNGZC010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_8844"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="AC16G0_01890"
     CDS             complement(<1..1077)
                     /locus_tag="AC16G0_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR5098401.1"
                     /translation="MATAKKYVFFFGKGKAEGRAEQKNLLGGKGANLHEMTRLGVPVP
                     PGFTISTEACVYYQDHRKQHPPGLWKQVEENLKKTEKAMGAVFGDPANPLLVSVRSGA
                     RVSMPGMMDTVLNLGLNDTTLKGLIARSQNERFAYDSYRRFLQMFGDVVLGIEKSAFE
                     RLLEGMKHAKKVKLDTELSAGDLKELVELFKKVVREKTGKDFPQEPKEQLRLAINAVF
                     ESWNNPRAVTYRKLHNISGDWGTAVNVQTMVFGNLGENSGTGVAFTRDPATGERRFFG
                     EYLMNAQGEDVVAGIRTPQPISELKKAKPKGYAELERIYQRLEKHYRDMLDIEFTIQD
                     GVLYMLQCRVGKRTAAAAVKIAVDM"
     gene            complement(1098..>2031)
                     /gene="glyS"
                     /locus_tag="AC16G0_01895"
     CDS             complement(1098..>2031)
                     /gene="glyS"
                     /locus_tag="AC16G0_01895"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HIR5098402.1"
                     /translation="PACRAAAERAAHLCKADLVTSLVKEFTSLQGIMGREYARRSGET
                     AEVAQAIEEHYLPRFAGDRVPASNAGAVVGLADRLDSIVGCFGVGLIPTGSEDPYALR
                     RAALGVVQILIQRGLRVSLGAMVDQALAGLTTLVTADPEKTRREVLDFLRGRIQVVLL
                     DRGFPADVVEAVLSASADDPLDAVRRAAALAALKREADFAELSTAFRRGVGIVPNGFD
                     RPVEPTRLVEGAERGLYGEATSIRAEVERLVAAQEYLPALQRIAALKPMVDMFFEEVL
                     VIGPDAELTQNRFAVLKAVGDLFARIADFTKLLG"
BASE COUNT          311 a          710 c          664 g          346 t
ORIGIN      
        1 catgtccacg gcgatcttga cggccgcggc cgcggtgcgc ttgcccacac ggcactgcag
       61 catatagagg acgccgtcct ggatggtgaa ctcgatgtcc agcatgtcgc ggtaatgctt
      121 ttcgagccgc tggtagatcc tctccagctc ggcgtagccc ttgggcttgg ccttcttcag
      181 ctcgctgatg ggctgggggg tccggatgcc ggccaccacg tcctcgccct gggcgttcat
      241 caggtactcg ccgaagaagc gtcgctcccc cgtggcgggg tcgcgggtga aggccacccc
      301 ggtcccggag ttctcaccca ggttcccgaa gaccatggtc tggacgttga ccgcggtgcc
      361 ccagtccccc gagatgttgt ggagcttgcg gtaggtgacg gcccgcgggt tgttccagga
      421 ctcgaagacg gcgttgatgg ccaggcgtag ctgctccttg ggctcctggg ggaagtcctt
      481 gccggtcttc tcccggacca ccttcttgaa cagctccacc agctccttca gatcgccggc
      541 gctgagctcg gtatccagct tcaccttctt ggcgtgcttc atcccctcga gcagccgctc
      601 gaaggcgctc ttctcgatgc ccagcaccac gtcgccgaac atctggagga agcggcggta
      661 ggagtcgtag gcgaaccgct cgttctggga gcgggcaatg agccccttca gggtggtgtc
      721 gttcaggccc aggttgagca cggtgtccat catgcctggc atggacaccc gggcgccgga
      781 tcggaccgag accagcaggg gattggcggg gtcgccgaag acggcgccca tggccttctc
      841 cgtcttcttg aggttctcct ccacctgctt ccacagcccc ggggggtgct gcttgcggtg
      901 atcctggtag tagacgcagg cctcggtcga gatggtgaag cccgggggca ccgggacccc
      961 gaggcgcgtc atctcgtgga ggttcgcgcc cttcccgccc agcagattct tctgctcggc
     1021 gcggccctcc gccttgccct tgccgaagaa gaagacatac ttctttgccg ttgccataga
     1081 gacctccgct tttcccctca tcccaagagt ttggtgaagt cggcaattcg cgcgaacaga
     1141 tcccccaccg ccttcagcac cgcgaagcga ttctgggtca gttccgcgtc tggtccgatg
     1201 accagcacct cttcgaagaa catgtccacc atgggcttga gggccgcgat ccgctgcagg
     1261 gcggggagat actcctgcgc ggccacgagc cgctccacct cagcccggat ggaagtggcc
     1321 tcgccgtaga ggccccgctc cgcgccctcc accaggcggg tgggctccac gggacggtcg
     1381 aagccgttgg gcacgatccc tacgccgcgg cggaaggcgg tggagagctc cgcgaaatcc
     1441 gcctcccgct tgagcgccgc cagggccgcg gcgcgccgca ccgcatccag agggtcatcc
     1501 gcgctggccg agagcacggc ctccacgaca tccgccggga atccccgatc cagcaagacc
     1561 acctggatcc ggccgcgcag gaaatccagg acctcgcgcc gggtcttctc ggggtccgcc
     1621 gtgaccagcg tcgtgagccc ggccagggcc tggtccacca tcgcgcccag agagacccgg
     1681 aggcctcgct ggatcaggat ctggaccacc ccaagcgccg cccgccgcaa ggcgtaggga
     1741 tcctccgagc ccgtggggat gaggccgacg ccgaagcagc ccacgatcga gtccagccgg
     1801 tccgccaggc ccaccaccgc ccccgcgttg gaggccggga cccgatcccc ggcaaagcgg
     1861 gggaggtaat gctcttcgat ggcctgggcg acctcggccg tctcgcccga gcgccgggcg
     1921 tactcgcgcc ccatgatgcc ctggagcgag gtgaattcct tcaccagcga ggtcaccagg
     1981 tccgccttgc agaggtgggc ggcccgctcc gcggcggcgc ggcaggcggg a
//
LOCUS       DBNGZC010000100         2439 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1475920, whole genome shotgun sequence.
ACCESSION   DBNGZC010000100 DBNGZC010000000
VERSION     DBNGZC010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1475920"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="AC16G0_01900"
     CDS             <1..272
                     /locus_tag="AC16G0_01900"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HIR5098403.1"
                     /translation="EGRYHADAEALARLKTQGQIVFRYSTPDGRLTDEANPNGSLEHI
                     AGVCNEGRNVLGLMPHPERAAEALLGSTDGIPLFRGILEALLPAA"
     gene            327..>2439
                     /gene="purL"
                     /locus_tag="AC16G0_01905"
     CDS             327..>2439
                     /gene="purL"
                     /locus_tag="AC16G0_01905"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HIR5098404.1"
                     /translation="MHEPGVITPELIAQHGLTPEEYQRVRQILGRVPNFTELGIFSVM
                     WSEHCSYKSSRVHLKQFPTHGECILQGPGENAGVVDIGDGLAIVFKIESHNHPSFIEP
                     YQGAATGVGGIIRDIFTMGARPIALLDPLRFGPLTEARTRYLVGGVVAGIAGYGNAVG
                     VPTVGGELCVSETYAGNPLVNVFCLGIAPKDRIFLARASGEGNPVIYVGAKTGRDGIH
                     GATMASETFDEGSEERRPTVQVGDPFREKLLIEACLELMAGDAVIGIQDMGAAGLTCS
                     TCEMASRGGTGIEIDLAEVPRRETGMIPYEVMLSESQERMLLVVRRGREPEVMSIFQK
                     WDLDAVIIGRVTGDGLMRVKDGPRVVAEIPAVALAEEGPVYHRPMQRPAYLAQTGEAD
                     LSSIPVPEKPDQVLLELLASPNIACKRPVWQQYDHMLFCNTVVEPGGDAAVLRVQGTR
                     KWLALSADGNGRYTYLDAYEGGKLAVAEAARNVVCAGARPLAITNCLNFGSPERPEIM
                     WQFAEAVRGMADACRAFGTPVTGGNVSFYNQTLTAAIFPTPVVGMVGLLDDIRQATTP
                     WFKVPGDVVLLLGETQDDLGGSEYLKVIHDLETGRPPRLDLDREQSVQQACLEAIRAG
                     AVRSAHDCSDGGLAVTLAECCMIGPEVRLGASLSLPGEMRPDALLFGESASRIVISAA
                     PDAVEGIRGIARRLAVPCAEMG"
BASE COUNT          392 a          773 c          900 g          374 t
ORIGIN      
        1 gcgaaggacg gtaccacgcg gacgccgaag cactcgcccg tctgaagacc cagggacaga
       61 tcgtcttccg ttacagcacc ccggacgggc gcctcacgga tgaggcgaac ccgaacgggt
      121 cgctcgagca tatcgccggg gtgtgtaacg aaggccgcaa cgtcctggga ctcatgccgc
      181 atccggagcg ggccgccgag gcccttctgg gttccacgga cggcataccc ctcttccgtg
      241 ggatcctgga ggctctcctc cccgccgcct gatgagtggg tcggaccggc cgatcatcag
      301 cgcacaccac gcagtcgagg aaccgcatgc acgaaccagg cgtcatcacg cccgagctta
      361 tcgcccagca tggtttgacc cccgaggagt accagcgggt ccggcagatc ctggggcgcg
      421 tcccgaactt cacggaattg gggattttct cggtgatgtg gtcggagcac tgcagctaca
      481 agagttcgcg ggtgcacctg aagcagttcc ccacgcatgg ggagtgcatc ctgcaagggc
      541 cgggcgagaa cgccggtgtg gtggacatcg gggacgggct ggcgatcgtc ttcaagatcg
      601 agagccacaa ccacccctcg ttcatcgagc cctaccaggg cgcggccacg ggcgtgggcg
      661 ggatcatccg ggacatcttc accatggggg cacggcccat cgcgctcttg gacccgctgc
      721 ggttcggccc gctcacggag gcgaggacgc gctacctggt ggggggcgtg gtggcgggga
      781 tcgcgggcta cgggaacgcg gtgggcgtcc cgacggtggg cggggaactc tgcgtctccg
      841 agacctacgc cgggaatccc ctggtgaacg tgttctgcct cggcatcgcc cccaaggacc
      901 ggatcttcct ggcgagggcc agcggcgagg gaaacccggt catctacgtc ggggccaaga
      961 cgggccggga tgggatccac ggggccacca tggcctccga gaccttcgac gaggggtccg
     1021 aggagcggcg gccgacggtc caggtagggg acccgttccg ggagaagctt ctcatcgagg
     1081 cctgcctgga gctgatggca ggggacgcgg tgatcggcat ccaggacatg ggggctgcgg
     1141 gactcacctg ctcgacgtgc gagatggcca gccgcggcgg cacggggatc gagatcgacc
     1201 tggccgaggt cccgcggcgc gagacgggaa tgatccccta cgaggtgatg ctctccgagt
     1261 cgcaggagcg gatgctcctg gtggtccggc gcgggcgaga gcccgaggtg atgtccatct
     1321 tccagaagtg ggatctggac gcggtgatca tcggccgggt gacgggtgac ggcctcatgc
     1381 gggtcaagga cggcccgcgg gtcgtggccg agattccggc cgtggcgctg gcggaggagg
     1441 gtccggtcta tcatcgcccc atgcagcgcc cggcctacct ggcgcagacg ggggaggccg
     1501 acttgagttc gatccctgtc cccgagaaac cggaccaggt cctgctggag ctcctggcct
     1561 cgcccaacat cgcctgcaag cgacccgtct ggcagcagta cgatcacatg ctcttctgca
     1621 acaccgtggt ggagccgggg ggggatgcgg cggtcctgcg cgtccagggg acgcggaagt
     1681 ggctggcgct ctcggccgac ggcaacggcc ggtacaccta tctggatgcc tacgagggcg
     1741 gaaagctggc ggtggcggag gcggcgcgga acgtcgtctg tgccggtgcg cgccccttgg
     1801 ccatcacgaa ctgtttgaac ttcggcagcc cggagcgtcc cgagatcatg tggcagttcg
     1861 ccgaggcggt gcgggggatg gcggacgcct gccgggcgtt cggcacccct gtgaccggcg
     1921 gcaacgtgag cttctacaac cagacgctca ctgcggccat cttcccaacc ccggtggtgg
     1981 gaatggtggg actgctggac gacatccgcc aggcgacgac gccgtggttc aaggtgccgg
     2041 gggatgtcgt gctcctcttg ggcgagacgc aggatgatct gggcgggagc gaatacctca
     2101 aggtcatcca cgacctggag acgggccgcc caccccggct cgacctggac agagaacaat
     2161 ccgtccagca ggcctgcctg gaggctatcc gggcaggggc ggtgcgctcg gcgcacgact
     2221 gctcggacgg gggtctggcc gtgacgctcg ccgaatgctg catgatcggg cccgaggtcc
     2281 ggctcggggc gagcctcagc ctgccggggg agatgcgccc ggacgccctg ctcttcgggg
     2341 aatcggcttc tcggatcgtg atcagcgccg cgccggacgc ggtcgagggg atccggggga
     2401 ttgcccgccg cctcgccgtc ccctgtgcgg agatgggcg
//
LOCUS       DBNGZC010000101         4393 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_986942, whole genome shotgun sequence.
ACCESSION   DBNGZC010000101 DBNGZC010000000
VERSION     DBNGZC010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_986942"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            331..1368
                     /locus_tag="AC16G0_01910"
     CDS             331..1368
                     /locus_tag="AC16G0_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098405.1"
                     /translation="MQIIRSWLFLLAAWLLLVGGLGWAPPAATAAETVNAYSIWPENW
                     ARPMFEEFEKATGIKVNFVRFSSGEALARVIAEKNNPRVDVLFGGPVETHAAGIKEGI
                     FEPYKPPTFSVLEARFKHPEGQWIAIADDPLVFMSNTKFLQEHKLKPPASWSDLLDPA
                     YKNMIQMADARTSGTAVTRIFSILEVNGRDETKAFDYMKKLRPNVQLYTKSGGGGTLP
                     VGLGQAGAGIFFIVDALDTKAKGYDVTISFPKEGIGSSAEAIALLKGAKNPGVGKKLI
                     DWATSPAMQSLYAKYKINFVPAHPDVKVEASLAEVLKGAKIFHIDDVFAGANRKRIVE
                     RWVSEVLPLSQ"
     gene            1478..3151
                     /locus_tag="AC16G0_01915"
     CDS             1478..3151
                     /locus_tag="AC16G0_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098406.1"
                     /translation="MRRTLRDPALSAAVLVLWGLLALFILFPLAALLVRTFTEGGHVT
                     LAGFLAILKDPSNRQAFWNSLLLAALVGVCGTVLGFLYAFTAVRANLPRGWSTALDAA
                     SLLPLISPPFTTAIAMIFSFGPRGLITYHLLGIQHFTVYGLHSTLFSETLTYFPIAYL
                     TLKPILAAIDPNVEDMAFSLGSSRWRVFRTVTLPLTVPGVANAFLLLFAASLADFATP
                     LILAGNSFPILPTQAYLQITGLFDLKGGAVLSFVLLVPALTVYLLQRFWVSRKSYVTI
                     TGKAGAQTSIKSLAPWARVPLLGACLLVAVVILYFYVLLFYASVVVALGANHAWTWHH
                     YRVIFTEGLKAIRDTLLIAGVGMPLGGLYGILLGYLVAKKIFPGRQAMEIVSMINYSL
                     PGTIVGIAYLIAFNDPPIVLTGTALIIVACYVFRYSPTGIRATVALLQQIDPSIEEAS
                     EGLGAGSGTTFRRITLPLILPAFFAGLGVVFIRSMTAISATIFLVSINWTLITVRILE
                     NMTELSLGPAAAFSVLVVVIVFLVIALIGLLLRRFRAPGAVEMTSILGG"
     gene            3153..4247
                     /locus_tag="AC16G0_01920"
     CDS             3153..4247
                     /locus_tag="AC16G0_01920"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5098407.1"
                     /translation="MTAAGIRIQDVSKRFAHRVKGEVYAVRDVSLTVQPAELLTLLGP
                     SGCGKTTTLRMIAGFEQPDAGRVFIGEQDVTGLMANQRNIGFVFQNYALFPHLSIFEN
                     VAYGLRVQRKSGDEIARAVGEVLSLVGLQGYDRQFPNQLSGGEQQRVALARAIVIRPR
                     VLLFDEPLSNLDAKLRVHMRGELRDLQKRLQITAVYVTHDQEEAMAISDRIAVMHQGR
                     IVQEGTAETLYHRPASAFVAQFIGRTNLLAGKVLSASPGGVEVDVAGVHLKISTDAAG
                     LKAGEAVQLVARPEIIDLKSGAQAGGIQGTVVSRTFLGEKAEYRVQVGADILQVASYN
                     PGPHGVLAPGQTVTLQLRTDGIPLLPGDAA"
BASE COUNT          690 a         1554 c         1328 g          821 t
ORIGIN      
        1 ggccgtggag ctacctcatg ctcctgggct atgcagtcat cttcctcggc acacatccgg
       61 gccgcatcct tggcctagac ggcccgctct cccggcgcct gagcccatcg gccctcgccg
      121 gtcgtcggtg ggcaatccta ctctccctgt tgacctgacg caccgggtac ctcacgtccc
      181 tcccgctccc cgcctccagg cgttccagcg acgtccgcgg cctggaacat ttcctacccg
      241 cgcgttgaca gcgtcctggg gccttgatat ggtgaccccg tcttcctgtc catctcacgc
      301 gagataattt cttcccgaaa ggaggctggc atgcaaatca tccgctcctg gctcttcctc
      361 ttggccgcct ggctcttgct cgtcggcgga ctggggtggg cacccccggc cgcgaccgca
      421 gccgagaccg tcaatgcgta ctccatctgg cccgagaact gggcccggcc catgttcgag
      481 gagttcgaga aggccacggg gatcaaggtg aacttcgtgc gcttctcctc gggcgaggcg
      541 ctggcccggg tgatcgcgga gaagaacaac ccgcgggtgg acgttctctt cggcggcccc
      601 gtggagacgc atgcggcggg catcaaggaa gggattttcg agccgtacaa accccccacc
      661 ttcagcgtcc tggaggcccg cttcaagcac cccgagggcc agtggatcgc catcgccgat
      721 gaccctctgg tcttcatgag caacaccaag ttcctgcaag agcacaagct gaagccgccg
      781 gcctcctgga gtgacctgct ggatccggcc tacaagaaca tgatccagat ggcggatgcc
      841 cgcacctccg gcaccgccgt gacccgcatc ttctccatcc tagaggtcaa cggccgcgac
      901 gagaccaagg ccttcgacta catgaagaag ctgcggccga atgtccagct ctacaccaag
      961 agcgggggtg ggggcacctt gccggtgggt ctgggccagg cgggggccgg catcttcttc
     1021 atcgtggatg ccctggacac caaagccaag ggctacgatg tcaccatcag cttccccaag
     1081 gagggcatcg ggagctcggc cgaggccatc gcccttctca agggggccaa gaatcccggc
     1141 gtggggaaga agctgatcga ttgggcaacc tcccccgcca tgcagtcact gtacgccaag
     1201 tacaagatca acttcgtgcc ggcccatccg gacgtgaagg tcgaagccag cctggccgaa
     1261 gtcctgaagg gcgccaagat cttccacatc gacgatgtct tcgccggcgc caatcgcaag
     1321 cgcatcgtgg agcgctgggt gagcgaggtg ctgccgctct cgcagtgaga cctgctcccg
     1381 cgacgaggga ccagccggcg cccatggcgc cggctggtcc ccgtgtgttt gaggtgcctt
     1441 gtcagtgact ggcctcgccg ccgcctggaa tcaaaacgtg cgtcgcaccc tcagggaccc
     1501 tgctctctcg gcggccgtcc tggtcctctg ggggctcctg gccctcttca tcctgttccc
     1561 gctggccgcg ctcctggtgc ggacattcac cgagggcggg catgtcacct tggccggttt
     1621 cttggcgatc ctcaaggacc cgagcaaccg ccaggccttt tggaacagcc tgctcctggc
     1681 cgccctggtg ggcgtctgcg ggacagtcct gggatttctc tacgccttca ccgccgtgcg
     1741 ggccaacctg cctcggggct ggagcacggc cctggacgcc gcctccctcc tccccctgat
     1801 ctcgcccccg ttcaccaccg ccatcgccat gatcttctcc ttcgggccgc ggggactcat
     1861 cacctatcac ctgctgggga tccagcactt caccgtgtac gggctccaca gcaccctctt
     1921 ttccgagacg ctcacctact tccccatcgc ctatctgacg ttgaagccga tcctggccgc
     1981 catcgacccg aacgtggaag acatggcctt cagccttggg agttcccgct ggagagtgtt
     2041 ccgcaccgtg accctcccct tgaccgtgcc gggcgtggcc aacgccttcc tcctcctctt
     2101 tgccgcctcg ctggcggatt ttgccacacc gctgattctg gcgggcaact cctttcccat
     2161 cctccccacg caggcctacc tccagatcac cggcctcttc gacctgaaag gcggggcggt
     2221 cctttccttc gtcctgctgg tgcccgccct gaccgtgtac ctgctccagc gcttctgggt
     2281 gagccgcaag tcctatgtca ccatcactgg aaaggcgggg gcccagacga gcatcaagag
     2341 tctcgcgccc tgggcgaggg taccgctcct cggcgcctgc ctgctggtgg ccgtggtgat
     2401 cctttacttc tacgttctgc tgttctatgc ctccgtcgtg gtcgccctgg gcgcgaacca
     2461 tgcctggacc tggcatcact atcgggtaat cttcaccgaa gggttgaagg cgattcgcga
     2521 cacgctgctc atcgccggcg tcgggatgcc cctgggggga ctctacggga tcctgctggg
     2581 gtacctggtg gccaagaaga tcttcccggg gcgccaggcc atggagatcg tctccatgat
     2641 caactactcc ctgcccggga ccatcgtggg gatcgcctac ctcatcgcct tcaacgatcc
     2701 ccccatcgtc ctcacgggga ccgccctcat catcgtggcg tgctacgtgt tccgctacag
     2761 ccccaccggc atccgggcca cggtggccct cttgcagcag atcgatccca gcatcgagga
     2821 ggcctcggag ggcctggggg ccgggagtgg caccaccttc cggcggatca ccctccccct
     2881 gatcctcccg gccttcttcg cggggctggg ggtggtcttc atccgctcca tgaccgccat
     2941 cagcgcgacg atcttcctgg tctccatcaa ctggaccctg atcacggtgc gcatcctgga
     3001 gaacatgacc gagctgtctc tcggcccggc cgcggccttc tcggtcctgg tggtggtcat
     3061 cgtctttctc gtcatcgccc tcatcggcct cctgctgcgg cgcttccgcg ctccgggcgc
     3121 ggtggaaatg acgagcatcc ttggagggta acgtgacggc agccgggatc cggatccagg
     3181 atgtctccaa gcgcttcgcg catcgggtga agggcgaagt ctacgccgtc cgggacgtga
     3241 gcctcacggt ccagccggcc gaactcctga ctctgctggg cccgtccggg tgtgggaaga
     3301 ccaccaccct gcggatgatc gccggcttcg agcaacccga cgcgggccgg gtgttcatcg
     3361 gcgagcagga cgtgacgggc ctcatggcca accagcggaa catcggcttc gtcttccaga
     3421 actacgccct cttcccccat ctctctatct tcgagaacgt ggcctacggt ctccgcgtcc
     3481 agcgaaaatc cggggacgag atcgccaggg ccgtgggcga ggtcctgtcc ctggtgggtc
     3541 tccagggata cgaccggcaa tttcccaatc agctctcggg cggcgagcag cagcgcgtgg
     3601 cgttggcccg ggccatcgtc attcgcccgc gggtcctgct cttcgacgaa ccgctgtcca
     3661 acctggacgc caagctccga gtgcacatgc ggggtgaact ccgcgacctg cagaagcggc
     3721 tccagatcac cgcggtgtac gtgacccatg accaggagga ggccatggcc atctccgacc
     3781 gcatcgcggt gatgcatcag ggccggatcg tccaggaggg caccgccgag acgctctacc
     3841 atcgccccgc gtccgcgttc gtggcccagt tcatcggccg gacgaacctg ctcgccggga
     3901 aggttctctc cgctagtccg ggaggcgtcg aggtagatgt ggcaggggta cacctcaaga
     3961 tctcgaccga cgctgccggc ctcaaggcgg gagaggcggt tcaactggtc gcccgccccg
     4021 agatcatcga cctcaagtcg ggggcgcagg caggcggcat ccaggggaca gtcgtgtcgc
     4081 ggacattcct gggcgagaag gcggaatacc gcgtccaggt gggcgcagac atcctccagg
     4141 tcgcaagcta caatccgggc ccgcacggcg tcctcgcgcc cggccagacg gtgacgcttc
     4201 agctccgcac cgacggcatc ccactcttgc cgggagatgc cgcatgagcg cccccccgaa
     4261 tttgtcacac ggccgccttg ctcctgcctc tcccggacag tgggagagga ttcctctgag
     4321 ggccatcgtc ctggcgctgc tcctcctccc cggccctgtc ctggcagcag gcgcggagat
     4381 cggaagagcg tcg
//
LOCUS       DBNGZC010000102         2787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2160080, whole genome shotgun sequence.
ACCESSION   DBNGZC010000102 DBNGZC010000000
VERSION     DBNGZC010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2160080"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..116)
                     /locus_tag="AC16G0_01925"
     CDS             complement(<1..116)
                     /locus_tag="AC16G0_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HIR5098408.1"
                     /translation="MFEGFKKFIARGSVMDLAVGVIIGAAFGAIVDSLVKDI"
     gene            complement(230..856)
                     /locus_tag="AC16G0_01930"
     CDS             complement(230..856)
                     /locus_tag="AC16G0_01930"
                     /inference="COORDINATES: protein motif:HMM:NF024879.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HIR5098409.1"
                     /translation="MRSTISLLLVVMVAGLGVAGCETIQENPKTAIGVGAGAAGGALV
                     GGLIGHNTTGVVVGGLVGSLAGGAIGYYLDRQDKTAAQAAADTGYQPSQGVVVRVDKV
                     VVDPSAVAPGGTEYLGVTYTILTPTPNQTVQVSETREVRHNGALVANPTSQFTRQNGT
                     FTSALPITLPANAARGTYEVTTTVTVGTLFIATPGERTSRGIATFAVK"
     gene            1257..2495
                     /locus_tag="AC16G0_01935"
     CDS             1257..2495
                     /locus_tag="AC16G0_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014000200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family RNA-guided transposase"
                     /protein_id="HIR5098410.1"
                     /translation="MDVILERCAGLDVHQATVTACLRLPDPGRTRTQIVQTFGATTPD
                     LLTLRDWLAAHQVTHVAMESTGVYWKPVYYVLEDTFTLLLVNAAHMANVPGRKTDVAD
                     CVWIAQLLECGLLRGSFVPPPAIRELRDLTRYRKALLQDRTREANRLHKVLQDAGIKL
                     ATVATDILGVSGRAMLTALVAGTTDAAVLADLARGKLRAKLPALRQALTGRFRGHHAF
                     LVTQLLAHLDYLDETITRLSDEIAGHLAPFAREVARVQTIPGVKQRTVENLVAEIGVD
                     MTRFPTAGHLTSWAGICPGNNESAGKRRRGRTRKGDRWLKITLIEAALAAIRVKGSAL
                     GARYRRICRHRGHKIAIVAVARAILEIVWHILTSATTYHELGAAYLDRRDREQAARRY
                     VRLLENLGHRVTLEPAPIAA"
BASE COUNT          462 a          952 c          895 g          478 t
ORIGIN      
        1 atgatgtcct tcaccagcga gtccactatc gcgccaaatg ccgccccgat aatgacgccg
       61 actgcaaggt ccatgacgct tccgcgggca atgaacttct tgaacccctc gaacatggca
      121 cacctccttg tcaggccccg gcatgcgccg agggctcggt ggatttcgta tcacacctct
      181 gctgcgattc gaagcctcaa gaaatccgcg gtgcccggcc gccgagtctc tacttcacgg
      241 cgaaggtcgc gattccgcgg gaggtccgct cgccgggggt cgcgataaag agggtgccga
      301 cggtcaccgt cgtcgtcacc tcatacgttc cgcgcgccgc attcgccggg agcgtgatgg
      361 gcagggcgct cgtgaatgtg ccgttttgcc gtgtgaactg cgaggtggga ttggccacca
      421 aggcgccgtt gtggcggacc tcgcgggtct cgctcacctg gaccgtctga ttgggggtgg
      481 gggtgagaat ggtgtaggtg acgcccagat attccgtccc accgggggcc accgcactcg
      541 ggtccaccac gaccttgtcc actcgcacga cgaccccctg cgatggctgg taaccggtat
      601 ccgccgcggc ctgggcggcc gtcttgtcct gacgatccag gtagtagccg atggccccac
      661 ccgccaggct gcccaccagg ccgcccacga cgacgccggt ggtattatgg ccgatgaggc
      721 cgcccaccag ggcgccgcca gccgcccccg cgcccacccc gatggcggtc ttcgggtttt
      781 cctggatagt ttcgcagccg gcaaccccta ggcccgccac catcacgacc aatagcaagc
      841 ttatcgtgga acgcatggct cgccctcctc tctcccctcc cttgatcatc gcgccggact
      901 cctggcgtct tctcggtgag cctgttttct ctggatgggc ctccgtcctg gaacgtagtc
      961 aaagggaagg ccaattgtca agggtcattt tcccctggga aaatggccgc ccggaccttt
     1021 ccgaggcgaa ttacttatca gtcgccatgg gcctatgccc acccacgcca catgaaaatg
     1081 agctatcctg tctgcgcgcc gtgcgccctg gtgaggtcgg taccccttgg taccctgagt
     1141 ccgcgatcag tctgccaccc gggagcttca ggtaccccgc cctgtcgcca cgagcacgcc
     1201 gagcagagtc tcgctctcga agctcctcgg ggcgccggcc gcacaaggag gagcccatgg
     1261 atgtcatcct ggaacgctgc gcgggtcttg atgtccacca agccacggtc acggcctgtc
     1321 tgcggctgcc cgatcccggt cggacacgca cgcaaatcgt ccagacgttc ggggcgacca
     1381 cgccggacct cttgacactc cgggactggc tggcggccca ccaagtgacg catgtggcca
     1441 tggagagtac gggggtgtac tggaagcccg tgtactacgt cctcgaggac accttcaccc
     1501 tcctcctcgt gaacgccgcc cacatggcga atgtgccggg gcggaagacc gatgtggccg
     1561 attgcgtctg gatcgcccag ctgctggagt gcgggctcct gcgggggagc ttcgtgccac
     1621 cgccggcgat ccgggagctc cgggacctca cgcggtaccg gaaggccctc ctccaggacc
     1681 ggacccggga ggccaaccgg ctccataagg tgctgcagga tgccggcatc aagctcgcca
     1741 ccgtggccac cgacatcctg ggcgtctcgg gccgggcaat gctgactgct ctggtcgcag
     1801 ggacgaccga cgccgcggtc ctcgccgacc tcgcccgagg gaaactccgg gccaagctcc
     1861 cggccttgcg gcaggctctc accgggcgct tccgggggca ccacgccttc ctggtgaccc
     1921 aactcctcgc gcacctggac tatctcgacg agaccatcac gaggctgagc gacgaaatcg
     1981 cgggtcacct ggcccctttc gcccgggagg tcgcccgcgt ccagacgatc ccgggggtga
     2041 agcaacggac ggtggagaat ctggtggccg agatcggggt ggacatgacg cgcttcccca
     2101 cggcggggca cctgacgagc tgggccggca tctgtcccgg caacaacgag agtgcgggga
     2161 aacgccgccg gggccgcacc cgcaaggggg accggtggct gaagatcacc ctgattgagg
     2221 cggccctcgc cgccatccgg gtcaagggtt ccgccctcgg ggcgcgctac cgccggatct
     2281 gccgccaccg cggccacaag atcgctatcg tcgccgtggc gcgggccatc ctggagatcg
     2341 tctggcatat cctcaccagc gcgaccacct atcacgaact cggggcggcc tacctcgatc
     2401 gccgcgacag ggagcaggcc gcccggcgtt acgtccgctt gctggagaat ctcggccatc
     2461 gcgtgacctt ggagccggca cccatcgccg cctgagaggt cggcatcacc tattttctga
     2521 gcagggggac acggatacca tcgagggtga agccagtgga aaggatggta cgggggagaa
     2581 ctccatcggg agtgaggcgg gtttgttggt cgaggacact aaatgccgcc agcctgaccc
     2641 gttcgtcgag atcccccgcg ctgctgtcca cttcagctcc tctgccgcga gagcgtgggt
     2701 accctccgca gtgtgaagcg agaagtgcaa aggccagtgg ttggcccaga gtgcgcgtcg
     2761 gcggtggtgt ggtggtggtc gctcgca
//
LOCUS       DBNGZC010000103         1632 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_986946, whole genome shotgun sequence.
ACCESSION   DBNGZC010000103 DBNGZC010000000
VERSION     DBNGZC010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_986946"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1117
                     /gene="leuB"
                     /locus_tag="AC16G0_01940"
     CDS             38..1117
                     /gene="leuB"
                     /locus_tag="AC16G0_01940"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008928478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR5098411.1"
                     /translation="MSIIAVLHGDGIGPEVTREAIKVLRAAALRFNLTLEFREAVIGG
                     TAYEATGTPLPDVTLALCRKADAILFGAVGGPRWDSLPRKVRPEQGILALRKALDLFA
                     NLRPAKLFAALTDASTLKPEVIQGVDLLVVRELTGGLYFGQPKGIQPVPEGERGVDTL
                     IYTTPEIQRIARMGFEAAGRRRKHLTSVDKANVLATSELWRRVVTEMGTAFPDVRLDH
                     MYVDNAAMQLIRQPTSFDVIVTENTFGDILSDQAAMLTGSIGLLPSASLGTGAGLYEP
                     IHGSAPDIAGQDRANPIAAILSAAMLLEYSLQAGSAAATIQAAVGQILDQGYRTPDLQ
                     QAGTQAVGTCRMGDLIAEAVRAGAA"
     gene            1235..>1632
                     /locus_tag="AC16G0_01945"
     CDS             1235..>1632
                     /locus_tag="AC16G0_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR5098412.1"
                     /translation="MAAQTSDILVEQSDTYARIIFNRPHVRNAISVRMWEELPKLIRR
                     LQGQRDVHAIVLSGAGGKAFAAGADIHELPHFAEPKTARTYETLVVRALDSLVRCPLP
                     VIAMIQGHAMGGGCHIATACDLRIAGESAHL"
BASE COUNT          295 a          572 c          523 g          242 t
ORIGIN      
        1 tcccacacgc gccggcggcg gcaatccgag gagtatcatg tccatcatcg ccgtgcttca
       61 cggtgacggc atcggccccg aggtcacgcg cgaagccatc aaggtgctcc gcgccgccgc
      121 cctgcgattc aacttgaccc tggaattccg cgaagcagtt atcggcggca ccgcctacga
      181 ggcaacaggc acacccttac ccgacgtcac cctagccctc tgccgaaagg ccgacgccat
      241 cctgttcggg gccgtgggcg gcccccgctg ggactccctg ccgcgaaagg ttcgtccgga
      301 acagggcatc ctggccctgc gcaaggccct cgacctcttc gccaacctgc gcccggcgaa
      361 gctctttgcc gctctgacgg acgcctcgac gttgaagcca gaggtaatcc aaggcgtgga
      421 ccttctggtg gtgcgggagc tgaccggggg actctacttc ggccagccga aggggatcca
      481 gccggtgcca gagggagaac gaggggtgga tactttgatc tacaccaccc cagagatcca
      541 gcgaatcgcc cggatgggct tcgaggcggc aggacggcga cgcaaacacc tcacgtccgt
      601 agacaaggcc aatgtcctgg ctacatccga gctctggcga cgggtcgtga ccgagatggg
      661 cacggccttc cccgacgttc gcctggacca catgtatgtt gacaacgccg ccatgcaact
      721 gatccgccag ccgacttcct ttgacgtgat cgtgaccgag aacacctttg gcgacatcct
      781 gagcgaccag gcggccatgc tcacgggctc gattgggctc ctcccctcgg cgagtctcgg
      841 gaccggcgcg gggctgtacg agcccatcca tgggagcgcg ccagatatcg cggggcaaga
      901 tcgggccaac cccatcgccg ccatcctctc ggccgcgatg ctgctcgaat attctctgca
      961 ggcggggtcc gcggcggcga cgatccaggc agccgtgggg cagatcctgg atcagggcta
     1021 tcggaccccg gacctccaac aggccggcac ccaggctgtc gggacctgcc ggatgggcga
     1081 cctcatcgcc gaggcggtac gggcgggcgc ggcgtagcaa cccacggaaa gaccgcggga
     1141 gatcccaccg tcggggggtg cagaggcggt tcggtgcacg gaagcgagga cggtcctggc
     1201 caaccgagga cggggcggac cgcaaggtgc gaacatggca gcacagacat cggacatcct
     1261 ggtcgagcag agcgacacct acgcccggat cattttcaat cgcccccacg tccggaacgc
     1321 catcagcgtg cgcatgtggg aggagctacc gaagctgatc cgccgcctcc agggccagcg
     1381 ggacgtgcac gccatcgtcc tcagcggcgc cggcggcaaa gccttcgccg cgggcgccga
     1441 cattcacgaa ctgccgcact tcgccgagcc caagaccgcc cggacctatg agaccctggt
     1501 cgttcgcgcc ctggacagcc tcgtgcgctg ccccctcccg gtgatcgcca tgatccaggg
     1561 acacgccatg ggcggcggct gtcacatcgc cacggcctgc gatctccgga tcgccggcga
     1621 atcggcccat ct
//
LOCUS       DBNGZC010000104         3131 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1084723, whole genome shotgun sequence.
ACCESSION   DBNGZC010000104 DBNGZC010000000
VERSION     DBNGZC010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1084723"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2026)
                     /locus_tag="AC16G0_01950"
     CDS             complement(<1..2026)
                     /locus_tag="AC16G0_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5098413.1"
                     /translation="MEIISTHVNADFDALASMVAASKLYPKARLLFPGSQERNVREFL
                     RETRFPLQAERLKGFSLDAVTRLILVDAKRGTRIGPLRDIVGRPGLEVHVYDHHPAHP
                     KDIQGSLEVLREVGATTTILLDLLREQELPVSPQEATVLALGIYEETGLLTFTTTTEA
                     DLQAAAFCLSRGANLGLVADFIRRELSAEQVGLLNDLIRSAETYTINGVRVVVSTASL
                     DRYVGDLAMLTHKLRDMENINVLFTLVRMDNRVHMVARSRLEAVDAGQIADAFGGGGH
                     ATAASATVKDLTLFQVKERLLQLLKVRVRPLKQARDIMTAPVKAIPERFTIRGAAEIM
                     NRFNLAYLPVVRRGEMVGLITREVVDKAMFHRLGDAPVHDYMSGEFHRVTPDTPLSQV
                     QRLMVERSLGFLPVLEGQRLKGAVTRADLLRHSYEDLLMRPTFPAAEERELGGPVARQ
                     MAGLLANRLPPRIQSTLRLAGTVADELGAKVYAVGGFVRDLLLQHENLDVDLVVEGDG
                     IAYAEVLGRHLSGKVTSHRRFGTAVVSLPDGFKIDVATARTEYYEYPAALPTVEHSSI
                     KMDLYRRDFTINTLAICLNADRYGELLDFFGGQQDLRDKTLRIIHNLSFVEDPTRILR
                     AVRFEVRFGFHLSRHAEQLITNAVQMGLLAKLSGARLFTELQLILQEARPL"
     gene            complement(2150..3007)
                     /locus_tag="AC16G0_01955"
     CDS             complement(2150..3007)
                     /locus_tag="AC16G0_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR5098414.1"
                     /translation="MAGRRGWFRRVGGEIVRHLQENDLMTMAAAISFYWLLGIVPLLL
                     LGTSAVGYFLGSSDRAADEVLAVTRRLLPRATGREVQEFLRGLIQSRHITGVLGIGFL
                     LWVAVGVFEIIATSLTALTGARDTRSFLRRKLVALVLMCTVGFLLILTLMGGWFLAAW
                     PNIQDLMGFRLVLPAFLTDRSFPHYFTTFLLGVALAIVYRVAPMRAIGWPAAVTGASI
                     AALLWHQAKTLFAWYVTHYSRVNIFFGILGGFVILVLWIFYTAIILLVGGILADVLDR
                     GGRPLKRDD"
BASE COUNT          573 a         1044 c         1020 g          494 t
ORIGIN      
        1 ccagggggcg cgcctcctgg aggatcaatt gcagttcggt gaacagccgg gcccccgaca
       61 gcttcgccag cagccccatc tggacggcat tggtgatgag ctgctcggcg tgccggctca
      121 gatgaaagcc gaaccgcacc tcgaaacgga ccgcgcgcag gatgcgcgtc gggtcctcga
      181 cgaagctcag gttgtggatg atgcggagcg tcttgtcgcg caggtcctgc tgcccgccga
      241 agaaatccag aagttctccg taccggtcgg cgttcaggca gatggccagg gtgttgatgg
      301 tgaagtcccg ccgatagagg tccatcttga tggagctgtg ctccaccgtg gggagggccg
      361 ccgggtattc gtaatactcg gtcctcgccg tcgccacgtc gatcttgaac ccatcgggca
      421 agctcaccac cgccgtgccg aagcgtcgat ggctagtgac cttccccgag aggtgccggc
      481 ccaggacctc ggcataggcg atgccgtccc cctccaccac caggtccacg tccaggttct
      541 cgtgctgcag caggaggtcc cgcacgaagc cgcccacggc gtacactttg gcgcccaact
      601 cgtcggccac cgtgcctgcc aaccgcaggg tggactggat ccggggaggc aggcgattgg
      661 ccaggaggcc agccatctgc cgggccaccg gcccccccag ctcacgctcc tcggcagccg
      721 ggaaggtcgg tcgcatcaag aggtcctcgt agctgtgacg aaggaggtcc gcccgggtca
      781 ccgccccctt gagccgctgc ccctccagca ccggcagaaa gcccaggctc cgctcgacca
      841 tgagacgctg tacctgcgag aggggcgtgt ccggcgtcac gcgatgaaat tccccggaca
      901 tgtagtcgtg gaccggcgca tcacccaggc ggtggaacat cgccttgtcc accacctcgc
      961 gggtgatgag gcccaccatc tctccgcggc gcaccaccgg gaggtacgcc aggttgaacc
     1021 ggttcattat ctccgccgcg ccccgaatcg tgaaccgctc cgggatggcc tttaccggcg
     1081 ccgtcatgat gtcccgcgcc tgcttgaggg ggcgcactcg caccttcaag agttggagca
     1141 gacgctcctt cacctggaac agggtgaggt ccttcaccgt ggccgaggcc gccgtggcgt
     1201 gacccccacc gccgaaggca tcggcgatct gccccgcatc caccgcctcc agtcggctgc
     1261 gcgcgaccat gtgcacccgg ttgtccatgc ggaccagcgt gaacaggacg ttgatgttct
     1321 ccatgtcccg cagcttgtgg gtgagcatgg ccagatcgcc cacgtagcga tccagggagg
     1381 ccgtgctcac caccacccgg accccgttga tcgtgtaggt ttcggccgac cggatgaggt
     1441 cgttcagcag ccccacctgc tcggccgaga gttctcgccg gatgaagtcc gcgaccaggc
     1501 cgaggttcgc ccctcgggac aaacagaagg ccgccgcctg caagtcggcc tcggtggtag
     1561 tggtgaaggt gaggagcccc gtctcctcgt agatgcccag ggcgagcacc gtagcctcct
     1621 gcgggctcac gggcagttcc tgctcgcgca ggaggtccag caggatggtg gtcgtggccc
     1681 ccacctcgcg taggacctcc aggctgccct gaatgtcttt ggggtgggcc gggtggtggt
     1741 cgtagacgtg cacctccagc cccggcctgc caacgatatc tcgcagcggc ccgatccgag
     1801 tgccccgctt cgcatcgacc aggatgaggc gggtgaccgc gtccagggag aaccccttca
     1861 gacgttccgc ctggagtggg aagcgggtct ctcgcaggaa ctcgcgcacg ttccgctctt
     1921 gcgagcccgg aaagagcagg cgggccttgg ggtagagctt cgacgccgcc accatggaag
     1981 ccagggcgtc gaaatcggcg ttcacgtggg tgctgatgat ttccatggag atgggcatcc
     2041 taccacgccc caccctgttc gccaatttcc ggaggtcgct ggaaggccag gggtgcccga
     2101 gccttctcac ccgcaggcag ggacctcgac ctcggtgccg cccgggccct cagtcatcac
     2161 gcttcagcgg gcggccccca cgatccagga cgtcggccag gatgccgccc acgagcagga
     2221 tgatggcggt atagaaaatc cacagcacca ggatgacgaa cccccccagg atgccgaaga
     2281 agatgttcac gcgggagtag tgcgtcacgt accaggcgaa caacgtcttg gcctgatgcc
     2341 agagcagggc ggcgatgctg gcgccggtga cggccgctgg ccagccaatg gcccgcatgg
     2401 gggcgacacg gtacacgatg gccagcgcca cgccgaggag gaaggtggtg aagtaatggg
     2461 gaaagcttcg gtcggtcagg aaggctggga gcaccaggcg gaatcccatg agatcctgga
     2521 tgttgggcca ggcggccagg aaccacccgc ccatcagggt caggatcagg aggaaaccca
     2581 cggtgcacat gagcaccagg gcgaccagct tccttcgaag gaacgaccgc gtgtccctgg
     2641 cgccggtgag cgccgtgagc gaggtggcga tgatctcgaa aacccccacg gccacccaga
     2701 gaaggaaccc gatgccgagc actcccgtga tgtggcgact ctgaatcagg cctcgcagga
     2761 attcctgcac ttcgcggccc gtcgcccggg gaagcaggcg gcgcgtcacg gcgagcacct
     2821 cgtccgcggc ccgatcggag gatcccagga agtagccgac ggccgacgtc ccgagaagca
     2881 ggaggggaac gatgccgagc agccagtaga aagaaatggc cgccgccatg gtcatgagat
     2941 cattctcctg gaggtgccgg acaatctcgc ccccaacccg acggaaccac cctcgtcgac
     3001 ccgccatttt atgcctccga tacccgaata tgccgcttag catagtcagc cttgtcctcc
     3061 agggcaagcc aaagccgcag cgtctgacgg atcggagcat gcatcgctca ctctggcgtg
     3121 agtggcgtcg a
//
LOCUS       DBNGZC010000105         1947 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_889077, whole genome shotgun sequence.
ACCESSION   DBNGZC010000105 DBNGZC010000000
VERSION     DBNGZC010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_889077"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..843
                     /locus_tag="AC16G0_01960"
     CDS             16..843
                     /locus_tag="AC16G0_01960"
                     /inference="COORDINATES: protein motif:HMM:NF024587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HIR5098415.1"
                     /translation="MAIIAISRGTFSGGEALAKRVAERLGYQCIGREMNLEVVTKDYD
                     ISPDGLMAAMEKRPSFLERVVGEHTVYLTCVRAALCGQAQGDRLVYHGYLGHLLLPGV
                     SHVIGVRVIADMEFRLQAVPPEKHFTREEAIAHIHKVDKDRREWTRFLFGVDWDDPHL
                     YAIVLNLSRISLDLACEMLVQVTERDEFQPTAASIKAMRDLTLSSQVSAKLARDFRTR
                     DADLKVTADDGIVTIVGTTPWAEIVDAVPVVARGVEGVKEVRTDVTGVTPLHPLNFY"
     gene            complement(940..1512)
                     /locus_tag="AC16G0_01965"
     CDS             complement(940..1512)
                     /locus_tag="AC16G0_01965"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013666959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5098416.1"
                     /translation="MQRHSRSLVMTRLTSLAVACLMPLMTASAAQGLDDLPYVPYVST
                     PEAVVLEMLEIANVSAKDIVYDLGSGDGRIVIAAAKRFGARGVGIEINPDLIREARDN
                     AEQAGVSHLVRFLEGDFFSADLREASVVTLYLLPEVNRQLLPKLLAELRPGTRIVSYK
                     YDLGSWAPEKTVPVSRGTVYYWVVPLGARR"
     gene            complement(1575..>1947)
                     /locus_tag="AC16G0_01970"
     CDS             complement(1575..>1947)
                     /locus_tag="AC16G0_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR5098417.1"
                     /translation="ISATLVKPALLQLPVLAGTALRIPMAGLVLWLTPWTRGTLDAIR
                     GSSRDERWRLATVCLLNAVGSALFTIAIRSGGVAIGNALASTAPLFAIPLEIGILKQR
                     PSRRTIVGAVCTVAGIACLSF"
BASE COUNT          387 a          598 c          644 g          318 t
ORIGIN      
        1 tcaaggagga gtccgatggc aatcatagcc atatcccgag ggacattcag tggcggggag
       61 gcgctcgcca agcgagtggc agagcggctg gggtatcagt gcatcggccg ggaaatgaat
      121 ctcgaggtgg taacgaagga ttatgacatc tcgccggacg gtctcatggc ggccatggag
      181 aaacggccct cgttcctgga acgggtggtc ggcgagcaca cggtctacct cacctgcgtg
      241 cgcgcggccc tgtgcgggca ggcccagggc gacaggctcg tctatcatgg ctacctcggg
      301 catctccttt taccaggcgt ctcccacgtc attggtgtgc gggtgatcgc cgatatggaa
      361 ttccgccttc aggcggtccc gccggagaag catttcaccc gcgaggaggc aatcgcccac
      421 atccacaagg tggataagga ccgacgggag tggacccggt tcctgtttgg ggtcgattgg
      481 gacgaccccc acctctacgc catcgtcctg aacctgagtc ggattagtct ggatctcgcc
      541 tgcgagatgc tggtccaagt gaccgagcgc gacgaattcc agccgaccgc cgcatcgata
      601 aaggccatga gggatctcac gctgagcagc caggtctcgg ccaagctggc cagggatttc
      661 cgcaccaggg atgccgatct gaaggtaacc gccgatgacg gaatagtgac catcgtcggc
      721 acgaccccgt gggcggagat agtggatgcc gtccccgtgg tcgctcgcgg ggtcgagggg
      781 gtaaaggaag tgcggactga cgtaaccgga gtcaccccac ttcaccctct gaacttctat
      841 taggaggcat gggctgaatc accctgagac atccaagaca agggtggccg agtatcctgc
      901 gtcaccaggc cgcacacccc cagaatcctc gtgtcagtct caccgccttg ctcccagcgg
      961 aacgacccag tagtagactg tgccccggct gacgggaacc gttttctcgg gagcccagct
     1021 ccccaggtca tacttgtacg aaacgattcg tgtgccaggc cgcagctcgg cgaggagctt
     1081 ggggagaagt tgccgattga cctccggcag aaggtacagc gtgaccacgc tcgcctctcg
     1141 caggtccgcg gagaagaagt cgccctcgag aaagcggacg aggtggctga ccccggcctg
     1201 ttcggcattg tccctggcct cgcggatgag atctggattg atttcgatac cgacgccgcg
     1261 ggcgccgaag cgcttggccg cggcgatgac gatgcggccg tcgccggagc cgaggtcata
     1321 gacgatgtcc ttcgcgctca cgttcgcaat ctccagcatc tccagcacca ccgcctcagg
     1381 cgtggacacg tagggtacat agggcaagtc atccaggccc tgcgccgccg aggccgtcat
     1441 gagcggcatg aggcaggcga cggcgaggga cgtgaggcgg gtcatgacca gggaccgaga
     1501 atgacgctgc atgcggagtt ccccgagcga acgtctcgga cggcgagttc agtaagggct
     1561 ggcatcggcg ccacctagaa gctcagacag gcgatcccgg cgaccgtgca caccgcccca
     1621 acaatggtgc gccttgaagg ccgctgcttc agaattccga tttccagcgg gatcgcgaac
     1681 agcggggcgg tggaggccag ggcattgccg atcgccacgc cgcccgagcg aatggcaatc
     1741 gtgaagagag ccgagcccac ggcattgaga aggcaaaccg tcgcgagccg ccagcgctca
     1801 tccctgctgc tgccgcggat agcatccagg gtgccccggg tccacggcgt cagccacaga
     1861 accaggcccg ccatggggat ccggagcgcg gtcccggcga gcaccggcag ttgaagcagg
     1921 gcgggcttga ccagggtggc cgagatg
//
LOCUS       DBNGZC010000106         3027 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_302365, whole genome shotgun sequence.
ACCESSION   DBNGZC010000106 DBNGZC010000000
VERSION     DBNGZC010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_302365"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="AC16G0_01975"
     CDS             complement(<1..983)
                     /locus_tag="AC16G0_01975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR5098418.1"
                     /translation="MKLDVRLGGTLSQAGRLAREAEAMGFDGAWTSETAHDAFLPLVL
                     AAEQTTRLTLGTSIAVAFPRSPMTTAYIAWDLQRLSGGRFILGLGSQVKGHIERRYGL
                     AWDAPGPRLRDYIRALRAIWDCWQQGTSLSYQGEFYLHSLMTPFFNPGQIDHPRIPIH
                     IAGVQEYMCRLAGELCDGLQVHPFHSEKYLREIVLPAVEQGLRASGRTRADVCLASSA
                     LVVTGETAARMQTARDDVRRQIAFYASTPNYRVILECHGWGQIAKELTRRSVRADWEG
                     MANLISDEMLEAFAIVGNPSEIPEKIRRRYAGLLDRLAIYETFDPGRNLAA"
     gene            complement(991..2436)
                     /locus_tag="AC16G0_01980"
     CDS             complement(991..2436)
                     /locus_tag="AC16G0_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HIR5098419.1"
                     /translation="MAYSDLREFIAALEQAGELRRITTEVDPILEVTEITDRVSKRGG
                     PALLFERPKGAAMPLLINAFGSPKRMALALQVKSLDDLAAELQEVLELKAPEGLLDKL
                     RMLPKLQELASAFPKVVKDGPCKEVIIRDDPSLAGIPILQCWPRDAGRYITFPLVFTR
                     DPETGVRNCGTYRMQVFDERTTAMHWHVQKGGAAHYRKAKRQGRRVEVGVAIGADPAV
                     CFAGTLPLPEGIDEMLVAGFIRKKPVELVRCETVDVEVPANAEIVLEGYVDTQELRRE
                     GPFGDHTGFYSLADDYPVFHLTCVTHRRRPVYHTIIVGRPPMEDCHMAAAIEQLFLPV
                     MRKQLPEIVDFHMPFEGIFHNLVLVSIDKQYPGHARKVMHALWGLGQAMVSKVIVVMD
                     REVNVRDYSEVTWKALNHIDPERDIEFVLGPVETLDHASRLPKYGSKMGVDATRKWPE
                     EGFTRPWPDEMVMDETTRRLVDQRWKDYGLE"
BASE COUNT          510 a          974 c         1004 g          539 t
ORIGIN      
        1 tgcgctgcca ggttgcggcc tggatcgaag gtctcgtaga tcgccagtcg gtccaggaga
       61 ccggcgtagc gccggcggat cttttccggg atttcactgg gattccccac gatggcgaag
      121 gcctccagca tctcgtcgct gatgaggttt gccattccct cccagtcggc gcgcacggac
      181 cggcgcgtca gctccttggc gatctggccc catccgtggc actcgaggat cacgcggtag
      241 ttaggcgtgg aggcgtagaa ggcgatctgg cgcctgacat catcccgtgc ggtctgcatt
      301 cgagcggcgg tctcgccggt cacgacgagg gcggagcttg ccaggcagac atccgcccgt
      361 gtcctgccgc tcgcccgcag accctgctcc acggcgggga gcacgatctc gcggaggtat
      421 ttttcggaat ggaaggggtg aacctggagc ccgtcgcaga gttcgcccgc cagtcggcac
      481 atatattcct ggacgccggc gatgtggatg gggatccgtg gatggtcgat ttggcccggg
      541 ttgaagaagg gggtcatcag actgtggagg tagaactccc cttggtagga gagcgatgtc
      601 ccctgctgcc agcaatccca gatggcgcgc agggcccgga tgtaatcacg gagacggggg
      661 cccggcgcat cccaggcaag gccgtaccgc cgctcgatgt gccccttcac ctggctgccg
      721 aggcccagga tgaaccggcc cccggagagg cgctggagat cccaggcgat gtacgccgtc
      781 gtcatggggc tccgggggaa cgccacggcg atgctggtcc ccagggtgag gcgcgtggtt
      841 tgctccgccg ccaggaccaa ggggaggaag gcatcgtggg ccgtctccga ggtccaggcc
      901 ccgtcgaagc ccatggcctc cgcctcccgc gccagcctcc ccgcctggga gagggttccg
      961 cccagccgaa cgtcgagttt catgcccgtc tcactccaga ccgtagtcct tccagcgctg
     1021 gtccacgagc ctccgggtcg tctcgtccat gaccatctcg tccggccagg gacgagtgaa
     1081 gccttcctcg ggccacttcc gggtggcgtc cacgcccatc ttggagccgt acttggggag
     1141 gcggctggcg tgatccaggg tctccacggg gcccaggacg aactcgatgt cccgctccgg
     1201 gtcgatgtgg ttcaaggcct tccaggtcac ctcggaatag tcacgcacat tcacctcgcg
     1261 gtccatgacg acgatgacct tgctcaccat ggcctgcccc agaccccaca gggcgtgcat
     1321 caccttgcga gcgtggccgg gatactgctt gtcgatgctg accagaacca ggttgtggaa
     1381 gatcccctcg aagggcatgt gaaagtccac gatctcggga agctgcttcc gcatgacggg
     1441 caggaagagc tgctcgatgg cggccgccat gtggcagtcc tccatggggg gtcgcccgac
     1501 gatgatggtg tggtacaccg ggcggcgccg gtgcgtcacg cacgtgaggt ggaaaaccgg
     1561 gtagtcgtcg gccagggagt agaagccggt gtggtcgccg aaggggccct cgcggcgcag
     1621 ctcctgggtg tccacgtagc cctccaggac gatctcggcg ttggccggaa cctccacgtc
     1681 cacggtctca cagcggacca gctccacggg ctttttccgg atgaagcccg cgaccagcat
     1741 ctcgtcgatc ccttcgggca ggggcagggt tccggcaaag cagacggcag gatcggcacc
     1801 gatggccacg cccacctcga cccggcggcc ctgccgcttt gccttccgat aatgggcggc
     1861 gccgcccttc tggacatgcc agtgcatggc cgtggtccgc tcgtcgaaaa cctgcatgcg
     1921 gtaggtcccg cagttccgga caccggtctc ggggtcccgg gtgaagacta gggggaaagt
     1981 gatatagcgg ccggcatccc ggggccagca ctggaggatg ggaatccccg ccagcgacgg
     2041 gtcatcacgg atgatgacct ccttgcacgg gccgtccttg acgaccttgg ggaaggcgct
     2101 ggccagctcc tgaagcttcg gcagcattcg cagcttgtcc agaagccctt ccggcgcctt
     2161 caactccagg acctcctgga gctccgccgc caggtcgtcc agggatttca cctggagagc
     2221 cagcgccatg cgcttcgggc tgccaaaggc gttgatgagc agcggcatgg cggccccttt
     2281 cggccgctcg aagagcaggg ccggccctcc ccgcttgctg acgcgatcgg tgatctcggt
     2341 cacctccagg atcgggtcca cctccgtcgt gatgcggcgc agctcgccgg cctgctccag
     2401 ggcggcgatg aactcccgca gatcactgta cgccatggct ctcctcgatg cgaatgatgg
     2461 gttccgccga cggggcatgg aagagacaag ggtgagcagc ctcacccgga accgaagtgc
     2521 acgtactcta gcacataccc ccataccggg aaaggggccc tgagatgccc gtcgcgctgt
     2581 ccacaggcca catggaacgc gatctcccct ctgcgggaag gtccccggag gggaggtgcg
     2641 gccgggaggt tggtggcgac cgcctctcgg tcgagtgcgc cttcgggtgc ccactcgcct
     2701 ctgatgctcc gcggcagttg gcccctgctc agcccatccg ccatacggat cgtgcatgtg
     2761 cacggacatc ggccgagggt catggatatc tcattaggat gagatatgtc gggtacgagt
     2821 cctgcaacct gttcgaccgc aagcgggcaa ctgcacagat accggccgag gaggccagac
     2881 acactttgaa tctacgctgg ggagccacaa cgccttctct ggattcgacc agagaaggcg
     2941 ttgttggtgt ttgaggactg cattcaccct ttttgccagg tcgcgtgatg attcctgggg
     3001 cttatgaaag ctgaggagag atccacc
//
LOCUS       DBNGZC010000107         5379 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2553051, whole genome shotgun sequence.
ACCESSION   DBNGZC010000107 DBNGZC010000000
VERSION     DBNGZC010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2553051"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(536..1174)
                     /locus_tag="AC16G0_01985"
     CDS             complement(536..1174)
                     /locus_tag="AC16G0_01985"
                     /inference="COORDINATES: protein motif:HMM:NF046206.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HIR5098420.1"
                     /translation="MLVGRRMRTSLITVTKDATLAQARELLHSHRIRHLPVVEEDRLV
                     GILTDRDIRQASPSSTAGISPDRTSAFLAQIPVRGAMVRDVRTVSPYTTIEEAARLMI
                     EHKIGCLPVTEENRLVGIITETDILGVFVDVMGISEPSTRIELTVPDRPGALADVTQI
                     FKVHQVNISSIVTMSHEEPGKRLAIIRLKTINPKPILKDLAAAGFEPPPLPQ"
     gene            complement(1301..2695)
                     /locus_tag="AC16G0_01990"
     CDS             complement(1301..2695)
                     /locus_tag="AC16G0_01990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5098421.1"
                     /translation="MTSPHQLEALAGGEGLGAYCRYRLGETQAMLRRVLQAPGGRVTA
                     AIAGRTLAGYAAIFQPGPEERWGQRRIPGLLELGALEVDRRFRRQGLARRLLQTTFVG
                     GELDQAIVVAPQDAGDWDLEFSGFSPREYQQVILRLFRRHGFAEFVTDDPLMAADPRN
                     FLLVRVGEKASPSLYQAFRALLAIAPPRVSEAQVGLQQQYLGSALTSVRQINHLPAEE
                     REAIYRSLIPPRVAELLGLDLATSLDARGNRLVTYLCPEDQAFVRIEVRRDPLDRDCV
                     FLLKLTQPTDEFLEVAFIIVNDLEAERFDVDRDPAGGTGGILSGIRNLAEELRAMRAG
                     LAPGQVRRGLRLFREALPLVESFAAELGREQISIEALFYHHAILYEHHGFGYLTGRDR
                     LEEIHRGFLPGGCLHRRLDGSTPFRRPEAAQTVRGRSWAIHDGILGEPWKVPRLYKIV
                     GRAMAMPTFPDFTY"
     gene            complement(2838..3455)
                     /locus_tag="AC16G0_01995"
     CDS             complement(2838..3455)
                     /locus_tag="AC16G0_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HIR5098422.1"
                     /translation="MFGLRLRRSAKFFDMFIEEARNIHQGAKAFQQMLEDFHGTDEQW
                     RAVEEYEHEGDKITHRILSVLHRTSSPVREREDIHKLAVTMDDVLDLIEGSAARMAMY
                     KVAKPTPEALRLAGIIVRCTDQIVQGVSALPRFANIQPHCIEINRLENEADEVSREAI
                     AALFVGEAAPIEVIKWKEIYETMESATDFGEDVANLLEEICLKYF"
     gene            complement(3465..4109)
                     /locus_tag="AC16G0_02000"
                     /pseudo
     CDS             complement(3465..4109)
                     /locus_tag="AC16G0_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250595.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
     gene            complement(4423..4848)
                     /locus_tag="AC16G0_02005"
     CDS             complement(4423..4848)
                     /locus_tag="AC16G0_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR5098423.1"
                     /translation="MAMSLPSRAADRRLLSEINVTPLVDVMLVLLVIFMVTTPLLQRG
                     TDVQLPQAQQSDLKEEERITLTLKRDVRLFLNDQEVPRASLRDRLVALARGRERVVHF
                     RGDAQVPYGMVIEVMDALKAAGIETVGMITERPSTAGRP"
     gene            complement(4859..>5379)
                     /locus_tag="AC16G0_02010"
     CDS             complement(4859..>5379)
                     /locus_tag="AC16G0_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR5098424.1"
                     /translation="DQGKLDLALSLCDHAKGSHVASVLAAGMRELLPIASGNPGLHAG
                     PSLDRASVPAREAMQRVTAQEIERLEQRLTSLATLGNISPFVGLFGTVIGVMRAFEAI
                     SRTGSGGLGTVSAGIAEALVATAAGLFVAIPAVTAYNHFVSRVKGCATEMDGATSELL
                     GRVQRAASSLGE"
BASE COUNT         1018 a         1745 c         1671 g          945 t
ORIGIN      
        1 aatgtgaaca ggagtgtaag gaggctaaca tctacccagg tgagactcat gactcgctcc
       61 aactgtcgcg attgcccaga cctcgtactc agcagtttga actatagcat aagcaaaata
      121 tatgccatcc ccaccgaagt atccggacga gactaagttc acttctaatt gagaggataa
      181 ctcgttgatt tttcggcgat ggcttctttg cctcttcgta gtgcctgtca tgtcgcaacc
      241 tctccctact gacaacatga atccacggct gagtccaatt gcgacagttt attcccaatt
      301 ccgcactttg gccgccgcca tctgcctcgc actcgcagga tcgggcaagg aaccgcggct
      361 acgacacgac tcatccaggg cgtcggggtc gcaagagtgc agaccggcac ccggagaaag
      421 gagcgccccc aacaaccgcc atcaggctat cttctggagg tctagacggg aacctgagga
      481 atttgagtcg gtcgaagcgc cgatggacgc cggatgctca gtccaccggc cgctttcatt
      541 gcggaagtgg cggcggttcg aatcctgcag cggccaagtc cttcaagatc ggtttcggat
      601 ttattgtctt aagccggatg atggccagcc gctttccggg ctcctcgtgg gacatggtga
      661 cgatgctgga aatgttgacc tgatgcacct tgaagatttg ggtcacatct gccagggccc
      721 ctggccgatc gggcaccgtg agttcaattc gagtgctcgg ctcgctgatt cccatgacgt
      781 ccacgaagac gcccagaata tcggtctcgg tgatgatacc caccaagcgg ttctcctcgg
      841 tgacaggcag acagccgatt ttgtgctcga tcatgagacg cgcggcctct tcgatggtgg
      901 tgtaggggct caccgtgcgg acgtcccgca ccatggcgcc cctgaccgga atctgggcca
      961 ggaatgcgga ggtccgatct gggctgatgc ccgcagtgct gctgggagag gcctgccgga
     1021 tgtcgcgatc cgtgaggatg ccgaccagcc ggtcttcctc caccaccggc aggtggcgga
     1081 tccgatggct gtggagaagt tcgcgcgcct gcgccaaggt ggcatccttg gtcaccgtga
     1141 tcagggacgt gcgcatcctt cggccgacca gcatgatgac ctccttgctc ccacccccat
     1201 tgtttggaaa gagccactgg gtcggactct ccccgcatgt agccccaggc cgcccggaca
     1261 ccccgcgcgc ggccggtgga atgcaggaag gttcgccgcc tcaatatgtg aagtccggaa
     1321 atgtgggcat ggccatggcc cgccccacga tcttatagag tcgtggcacc ttccagggct
     1381 cccccaggat cccatcgtgg atggcccaac tcctcccgcg gaccgtctgt gccgcctcgg
     1441 gccttcggaa cggcgtcgat ccgtccagtc ggcggtggag acatcctccg ggcaagaagc
     1501 cccggtgtat ctcctccaag cgatcacgcc cggtcagata gccgaatcca tggtgctcgt
     1561 acaggatggc gtgatggtaa aagagcgctt cgatggagat ctgctccctg cccagctcgg
     1621 cggcaaaaga ctccaccagg gggagggcct cacggaacag tcgcaggcct cgccgcacct
     1681 gccccggggc cagaccggcg cgcatggccc ggagttcctc ggcgagattc cggatgcctg
     1741 acaaaatgcc cccagtccct cccgcggggt cgcggtccac atcgaatcgt tcggcctcca
     1801 ggtcgttcac gatgatgaag gcgacctcta gaaattcatc cgtgggctgg gtgagcttca
     1861 acagaaacac gcagtcccga tccagtggat ctcggcggac ctcgatccgg acgaaagcct
     1921 gatcttcggg acacaggtaa gtgacaagcc gattcccccg ggcatccagg ctggtggcca
     1981 gatccaatcc caaaagctcc gccacccgcg gcggaatcag ggatcggtag atcgcctcac
     2041 gctcctcggc gggcaggtga ttgatctgac gcacggacgt gagcgcggag cccaggtatt
     2101 gctgttgaag accgacctgc gcctcgctca cccgcggtgg cgcgatcgcc aagagggcac
     2161 ggaaggcctg gtagaggctg ggcgatgctt tttctcccac gcggaccaag aggaagttac
     2221 gggggtcggc cgccatgaga ggatcgtccg ttacaaattc cgcaaagcca tgccggcgga
     2281 agagccggag gatcacctgc tggtactctc gcggcgagaa gccagagaac tccaggtccc
     2341 aatcgcccgc atcctggggt gcgaccacga tggcctgatc cagttctccc ccgacgaagg
     2401 tcgtctgcag gagacgtcgt gccagaccct gtcggcgaaa tcgccgatcc acctccaagg
     2461 cgcccaactc aaggagtccc gggatccgcc gctggcccca ccgctcttcc ggtcccggtt
     2521 ggaagatggc cgcgtatccg gccagggtcc ggccggcaat ggcggcagtc acgcggccgc
     2581 ccggcgcttg gagcacccgc cggagcatcg cctgtgtctc cccaagccgg tagcggcaat
     2641 acgcgcccaa gccctccccc ccagcgagcg cctccagttg gtggggggac gtcaagatgc
     2701 gcagatcgat cccatcccct ggcgtctgat gtactcccga gctctcggca ggcaagaatg
     2761 cggacggcat ggaacgcgaa tcccccgcgg cccggccgcc cgcaccgagc atgacgaacc
     2821 ggccgctttc ccccggatca gaaatacttc aagcagattt cctcgaggag gttggcgacg
     2881 tcctccccaa agtccgttgc gctctccatc gtctcgtaga tctccttcca cttgatgacc
     2941 tcgatggggg ccgcctcccc gacaaagagg gcggcgatgg cctctcgcga gacttcgtcc
     3001 gcctcgttct ccaggcggtt gatctcgatg cagtgcggct gaatgttggc gaagcggggg
     3061 agagcactga ccccctgcac aatttggtcg gtgcagcgga cgatgatccc ggccaggcgc
     3121 aaggcctccg gggtcggctt ggccaccttg tacatggcca tccgcgccgc cgagccctcg
     3181 atgaggtcga ggacgtcgtc catggtcacg gctagcttgt ggatgtcttc gcgctcccgc
     3241 acgggggagg aggtacgatg caggaccgag aggattcggt gggtgatctt gtccccctcg
     3301 tgctcgtact cctctacggc ccgccactgc tcgtcggtgc cgtgaaaatc ttccagcatc
     3361 tgctggaacg ccttggcccc ttggtgaatg ttccgcgcct cttcgatgaa catgtcgaag
     3421 aacttggcgc tgcgcctgag acgaaggcca aacatggtgt caccctactc ctctcctggc
     3481 tggggcactc ggcgcggcca gtggcgccag acgaaccagg ccgcggccga gaccagaccg
     3541 atgacgatga caacgtccaa ggagtgcagc acgcccccga cctgctccca gtgctccccc
     3601 aggagcatcc ccacgtacgc caggataaag ctccaaggaa gcgagccgag gaaggcatag
     3661 acgaggaatc ggcctatggg gattcgtgcg actcccgccg ggagcgagat gaatgtccgg
     3721 atcactggga ggagccggct gatgaacgtc gccgcaatcc cgtagcgtgc gaaccagcga
     3781 tccgcgcgct ccacctcgtg agggctgatc aggacgtacc ggccgtatgt aagaatgaac
     3841 tgccgtcctc cttaaacacc cacagcgtag gccgctgcgg agccaatggt gcaccccagg
     3901 gcccccgcca gagagactcc ccacaacgtg aagcgtccct ggctcacgag gtaccccgag
     3961 aatggcatga tgacctcgct gggcagggga atgcaggcgc tctcgatggc catggccaac
     4021 acgatccccc cgtagcccag gctgccgatg atccagatga caaaattgga gacaagggca
     4081 agaatctggc tgaagagatc catgggcatc tcgccgtcgc cggtcagtga ccgtgcatgt
     4141 tgctctatcg gttcccgatt ccgactgcag gctgggccga gcttggccta gcaggtcgcc
     4201 gaaaaccccc gcgtcgcgga ggctgctcga agagtttcaa ctgcaaggcg gcgcgcccat
     4261 cttcccatgg gtacccggcg cgggataccc accgaagggg gggtggaagc atactgcgtc
     4321 cgcatgtcgt cgttccgtgc gagcgcacca ggtacccact cggagaatga gcacccatcc
     4381 actgggtggg ttcccggcag atgggcctta atccgcagcc tcctaggggc gcccggcggt
     4441 cgagggacgc tcggtgatca tccccacggt ctcgatgccg gcagccttca aggcatccat
     4501 gacttcgatc accatgccgt agggcacctg ggcgtcaccg cggaagtgga cgacccgctc
     4561 ccggccccgc gccagggcaa ccaatcggtc gcggaggctt gcgcgaggga cctcttggtc
     4621 gttgaggaag agccgcacgt cgcgtttgag cgtgagggtg atccgctcct cctccttcaa
     4681 gtccgactgc tgggcctgag gaagctggac atcggtcccc cgctggagca gcggggtcgt
     4741 aaccatgaag ataaccagga gaaccagcat cacgtccacc agcggcgtga cgttgatctc
     4801 ggagaggagg cgccggtccg cggcgcggga ggggagggac atcgccatgg gatctcgctc
     4861 actcgccgag cgacgaagcg gcgcgctgaa cccgcccgag gagttcggag gtggccccgt
     4921 ccatctcggt ggcgcagccc ttgactcgcg aaacgaagtg attgtaggca gtcacggccg
     4981 ggatggccac gaacaggccg gccgccgtgg cgaccagagc ctcggcgatc cccgcagaga
     5041 ctgtccccag ccccccgctt cccgttcggg agattgcctc gaacgcccgc ataaccccga
     5101 tcacggttcc gaagaggccc acgaacgggc tgatgttccc cagggtggcg agggaggtca
     5161 ggcgctgctc cagtcgttcg atctcctggg ccgtgacccg ctgcatcgcc tctcttgcag
     5221 gtacgctggc ccgatccagt gaggggccgg cgtggagccc tgggtttccc gacgcgatcg
     5281 gcagcaactc ccgcatgccg gccgccagga ccgacgcgac gtggcttcct ttggcgtggt
     5341 cacacaggga cagggcgaga tccagtttcc cttgatcca
//
LOCUS       DBNGZC010000108         1636 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1573536, whole genome shotgun sequence.
ACCESSION   DBNGZC010000108 DBNGZC010000000
VERSION     DBNGZC010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1573536"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="AC16G0_02015"
     CDS             <1..213
                     /locus_tag="AC16G0_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HIR5098425.1"
                     /translation="DSLAKSLIVFGVVLALLGGVLLMAGKIPFLGRLPGDIFVRREHW
                     SFYFPLTTSILISVLLTLLFSLFSRR"
     gene            210..1379
                     /locus_tag="AC16G0_02020"
     CDS             210..1379
                     /locus_tag="AC16G0_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HIR5098426.1"
                     /translation="MSRPPALRPRRTLALAALILCLPALAGARIAEAQPVRVLLAEGR
                     TTAAVASAGGITITDPTGRRILAQQANGVPFRLVPRHSGVMVREAGVVAPILLVWPGR
                     IPTLDLDGQSYRGRLEVLPVNGGLTVVNVVELEDYLQGVLKDEIPPGWPAEAAKAVAI
                     AARTYAVYQRQQNPGALFYLRATTASQVYRGARGEDARTSWAVQATRGQILTFGGSPI
                     SAFYHSCSGGATEDAMDVFGLNFDTIVGVKDDFSLNCPYAFWVERLTAQEVQRALTQA
                     GYPVGRLLRLHVLARSRTGRILRLAVEHTRGQLVLEGKRFREALGNELLRSTDFEVQN
                     DRSGFTFIGRGAGHGVGLSQWGAKEMADLAYQYQEILKYYYPLAEIRSMPESAGP"
     gene            1421..>1636
                     /locus_tag="AC16G0_02025"
     CDS             1421..>1636
                     /locus_tag="AC16G0_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HIR5098427.1"
                     /translation="MQLSDFDYELPPDLIAQEPAAQRDAARLMVLDRPTGRIQHRRFS
                     DLPEYLRSGDLLILNDTKVIPARLYGVF"
BASE COUNT          251 a          536 c          536 g          313 t
ORIGIN      
        1 gattcgctgg caaaatcgct gatcgtcttc ggggtggttc tggccctgct gggcggtgtg
       61 cttctgatgg ccggaaagat ccccttcctc ggccgattgc ccggcgacat cttcgtccgt
      121 cgtgagcact ggagcttcta ctttcccctg acgaccagca ttctgatcag cgtcttgctc
      181 accttgctct tctcgctgtt cagccgccga tgagtcggcc accggccctt cggccaaggc
      241 gaactctcgc gttggcggcg ctgatcctct gcctcccggc ccttgccggc gcgaggattg
      301 ccgaggcgca gcccgttcgt gtcctcctag cggaggggcg gacgactgcc gcggtagcca
      361 gcgctggcgg catcacgatc acggatccca cgggacggcg catcctggcg cagcaggcga
      421 acggcgtccc attccggctg gttccgcggc acagcggcgt catggttcgc gaggcgggag
      481 tcgtggcgcc gatcctcctc gtctggcctg gacgcatccc cacgctggac ttggacgggc
      541 aatcctaccg tggccggctc gaggtgctgc ccgtgaacgg tgggctgacc gtggtgaacg
      601 tcgtggagtt ggaggactac ctgcagggcg tgctcaagga cgagattcct cccggctggc
      661 cggccgaagc ggcgaaggcg gtggccatcg cggcccggac ctatgccgtc tatcagcggc
      721 agcagaatcc gggcgccctg ttttacctgc gagccaccac ggcatcccag gtgtaccgcg
      781 gggcgcgcgg agaggacgcc cgtacctcct gggcggtgca ggcaacccgc ggacagatct
      841 tgaccttcgg gggctcgccc atctcggcct tctatcattc ctgctcgggt ggggccaccg
      901 aggatgccat ggacgtgttc ggcctcaact tcgacaccat cgtcggggtg aaggacgact
      961 tcagcctcaa ctgcccatac gccttctggg tggaacgctt gaccgctcaa gaggtgcagc
     1021 gcgcgctcac ccaggcaggg taccccgtcg gccgactcct tcgcctccat gtcttggcgc
     1081 gcagtcgtac aggtcggatc ctgcgcctgg cggtcgagca cacgcggggg cagctcgtcc
     1141 tggaggggaa acgcttccgg gaagcgctgg ggaatgagct tctccggagc actgacttcg
     1201 aggtgcagaa tgaccgcagc gggttcacgt tcatcgggcg cggggcgggg cacggggtcg
     1261 gtctgagcca gtggggggcc aaggagatgg ccgacctggc ctatcaatat caggaaatcc
     1321 tgaaatacta ttaccccttg gccgagatcc ggtccatgcc tgaatctgcc gggccgtaag
     1381 cccgagccgt cgaattgtcg aacaccctcc ggtttgtgcg atgcaactga gcgatttcga
     1441 ttacgagctg ccaccagatc tcatcgcgca ggagccggcg gcccagcggg atgctgcgcg
     1501 cctgatggtc ctggaccggc cgaccggccg catccagcat cgccgttttt ctgacttgcc
     1561 cgagtacctc cgctccggcg atctcctgat cctgaacgat accaaggtga tccccgcgcg
     1621 gctttacggg gtcttt
//
LOCUS       DBNGZC010000109         2088 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_204569, whole genome shotgun sequence.
ACCESSION   DBNGZC010000109 DBNGZC010000000
VERSION     DBNGZC010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_204569"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="AC16G0_02030"
     CDS             complement(<1..304)
                     /locus_tag="AC16G0_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR5098428.1"
                     /translation="MLDKFREACGVFGIIGHPEAANLTYLGLYALQHRGQESAGIVVA
                     DEDGLHLEKGMGLVADVFTEAKLRRLKGSLAIGHVRYSTTGSSQLKNAQPIVASYRR"
     gene            complement(359..1525)
                     /gene="alaC"
                     /locus_tag="AC16G0_02035"
     CDS             complement(359..1525)
                     /gene="alaC"
                     /locus_tag="AC16G0_02035"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HIR5098429.1"
                     /translation="MEEFKRIKRLPPYVFAIVNELKVKARARGEDIIDFGMGNPDLAT
                     PDHIVDKLIEAARNPRNHRYSASRGITKLRHAVCDWYRRRYGVELDPETEAIATIGAK
                     EGLSHLALAIVEPGDVALVPNPTYPIHAYSVVIAGGDVRHVRLGPDEDFFANLLAAFR
                     ESWPQPKLLILSFPHNPTAAVVEPDFFAKVVAFAQDNDLIVVHDFAYADLTFDGYQAP
                     SFLQTPGAKEIGVEFFTLSKSYNMPGWRVGFAVGNPRIIAALTRIKSYLDYGMFQPIQ
                     IAAIIALNGPQECVEEIRRTYASRRDALCDGLTRVGWKVDRPRGTMFVWARIPDQFRI
                     MGSLEFSKFLLDQAKVAVSPGIGFGQYGDEYVRFALVENEQRTRQAVQGIKRVL"
     gene            complement(1525..>2088)
                     /locus_tag="AC16G0_02040"
     CDS             complement(1525..>2088)
                     /locus_tag="AC16G0_02040"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIR5098430.1"
                     /translation="SVARDDLPDGGAGQFAATVRAIQELNPGAQVEVLVPDFKASAES
                     IRTVVASRPTIFNHNLETIERLTRQVRVQARYQRSLEVLRLAKAMGQPITKTGIMLGL
                     GEREDELLGLFRDARAVGCDVLTVGQYLQPTKAHLPVVEFVHPDRFKALEVAAYALGF
                     KAVYSGPLVRSSYHAELTARKAEETAA"
BASE COUNT          331 a          729 c          677 g          351 t
ORIGIN      
        1 cccggcgata gctggccacg atgggctggg cgttcttgag ctgcgagctg cccgtggtcg
       61 agtatcggac gtggccgatg gcgaggctgc ccttcaggcg gcggagcttc gcctcggtga
      121 agacgtccgc caccaggccc atacccttct cgaggtggag tccgtcctcg tcggccacga
      181 cgatcccggc actctcctgc ccccgatgct gcagcgcata gagcccgaga taggtcagat
      241 tcgccgcctc gggatggccg atgatcccga agaccccgca cgcctcgcga aacttgtcca
      301 gcatcctgcc cccctgccca cctctgatgc gccgcgccat ccgacgcggt cttccagttc
      361 acagcacccg cttgatgccc tggacggcct gccgggtccg ctgctcgttc tccaccaggg
      421 cgaagcgcac gtactcgtcc ccgtactgtc caaagccgat cccgggggag accgccacct
      481 tggcctggtc cagcaggaac ttgctgaact cgagggagcc catgattcgg aactggtccg
      541 ggatccgggc ccacacgaac atcgtccccc ggggcctgtc gaccttccat cccacccggg
      601 tcagcccgtc gcagagcgcg tcccggcgcg acgcgtaggt ccgccggatc tcctccacgc
      661 actcctgcgg cccgttcagc gcgatgatcg cggcaatctg gatgggctgg aacatcccgt
      721 agtccaggta gctcttgatc cgggtgaggg ccgcgatgat gcgaggattg cccaccgcga
      781 atcccacccg ccagccgggc atgttgtagc tcttggagag cgtgaagaac tcaaccccga
      841 tctccttggc cccgggcgtc tgcaggaagc tgggcgcctg gtacccgtcg aaggtgagat
      901 ccgcgtaggc gaagtcgtgc acgacgatca ggtcgttgtc ctgggcgaag gccaccacct
      961 tggcaaagaa gtccggctcg accaccgccg ccgtggggtt gtgcggaaag ctcaggatca
     1021 ggagcttggg ctgcggccac gactcgcgga acgcggccag cagattggcg aaaaagtcct
     1081 catccggccc caggcgcacg tgccggacat cgccgccggc gatcactacc gaataggcgt
     1141 gaatcgggta ggtggggttg ggcaccagcg ccacatcgcc cggctcgacg atcgccaggg
     1201 ccagatgcga caggccctcc ttggccccga tggtggcgat ggcctcggtc tcggggtcca
     1261 gctcgacccc gtagcggcgc cggtaccagt cgcagacggc atgccggagc ttggtgatcc
     1321 cgcgggaggc cgagtagcga tggttacgcg ggttccgcgc tgcctcgatc agcttgtcca
     1381 cgatatggtc gggggtggcg aggtctgggt tacccatccc gaaatcgatg atgtcctcgc
     1441 cccgggcgcg cgccttgacc ttcagctcgt tgacgatggc gaacacgtaa ggcggcagcc
     1501 gcttgatgcg cttgaactct tccatcaggc cgcagtctcc tccgccttgc gcgctgtcaa
     1561 ctccgcgtgg taggagctcc gaaccagggg gcccgaatac accgccttga accccagggc
     1621 gtaggccgcc acctccagcg ccttgaaccg gtcggggtgc acgaactcca cgacgggcag
     1681 gtgcgccttg gtgggttgca ggtactgccc gacggtcagg acgtcgcagc cgacggcccg
     1741 ggcatcccgg aacaggccca ggagctcgtc ctcgcgctcg cccagcccca gcatgatgcc
     1801 ggtcttggtg atcggctgcc ccatcgcctt ggccagtcgc agcacctcca gtgaccgctg
     1861 gtagcgggcc tggacgcgca cctgccgggt cagccgctcg atggtctcga ggttatggtt
     1921 gaagatcgtg gggcggctcg ccaccaccgt gcggatgctc tcggcgctcg ccttgaaatc
     1981 cgggaccagg acttcgacct gggcgccagg gttcaactct tggatggccc ggaccgtcgc
     2041 cgcgaactgc cccgccccgc cgtccgggag gtcatcccgc gccaccga
//
LOCUS       DBNGZC010000110         2985 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2454952, whole genome shotgun sequence.
ACCESSION   DBNGZC010000110 DBNGZC010000000
VERSION     DBNGZC010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2454952"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..935)
                     /gene="cysE"
                     /locus_tag="AC16G0_02045"
     CDS             complement(231..935)
                     /gene="cysE"
                     /locus_tag="AC16G0_02045"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003411985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - L-serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HIR5098431.1"
                     /translation="MFQTLRRDIRSVLERDPAARGATEILFCYPGLHAVWGYRVSHWL
                     WRHGFTLLARWVSHLARALTGIEIHPGAVIGPGFFIDHGMGVVIGETAEVGADVTLYH
                     GVTLGGTSLAKGKRHPTLRDRVVVGAGAKILGAITIGEDSRIGANAVVVKSVPANSVV
                     VGVPGQIVRRSKPHTAQDAPDLEHTVMPDTIGLALRALMTRVDALENRGGKGSEVGHT
                     PPALDLATSRGEDYSI"
     gene            complement(985..1917)
                     /gene="cysK"
                     /locus_tag="AC16G0_02050"
     CDS             complement(985..1917)
                     /gene="cysK"
                     /locus_tag="AC16G0_02050"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015290593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019344 - L-cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HIR5098432.1"
                     /translation="MRIANDVTELVGNTPLVRLQSLTEGCVADVVAKLEFFNPAHSVK
                     DRIGVAMIEAAEEVEAIRPDTIILEPTSGNTGIALAFVCAARGYKCVLTMPESMSKER
                     RMLLRAFGAELVLTPASQGMAGAIRKAEEMAAADPRYFIPQQFKNPANPEVHRRTTAE
                     EIWRDTDGTADILVAGVGTGGTITGVGEVLKARKPSFKCVAVEPEASAVLSGGPKGPH
                     AIQGIGAGFVPDILNVKIYDEIIRVKNDDAFTTARRLAREEGLLVGISSGAATWAALE
                     VARRRENARKLVVVIIPSFGERYLSTPLFADLAE"
     gene            complement(1951..2526)
                     /locus_tag="AC16G0_02055"
     CDS             complement(1951..2526)
                     /locus_tag="AC16G0_02055"
                     /inference="COORDINATES: protein motif:HMM:NF018696.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HIR5098433.1"
                     /translation="MSPTRPDLQVILQMVPEGARVLDLGCGDGTLLAQLEAHKAALAR
                     GVEVDEANVRACVAKGLSVRHGNIEDGLADFPEGMFDFVILSQTLAYLNRPLAVLKEM
                     LRVGRSAVVSLDNAGYWRARWRALCGDGAGTTLASREPRVRSITLGQLQEALGVLDLK
                     AEGRVFLRGQHRVCVWPALGAETAVFVITKA"
     gene            complement(2523..>2985)
                     /locus_tag="AC16G0_02060"
     CDS             complement(2523..>2985)
                     /locus_tag="AC16G0_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004078806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098434.1"
                     /translation="GKRFTARFDANSFLYITKAIDYFDLSLGQADLAAALTGIQAKFL
                     VLSYSSDWLYPPEQSEELVRALLRNGIDATYVEIQSDYGHDAFLLEVDRLGELARDFL
                     AKLERGATALRSAAGRPDTTTGGERVAPLAPWSAKQRGPVSLNGASRRESA"
BASE COUNT          496 a         1062 c          902 g          525 t
ORIGIN      
        1 atgagtttgt cctccagatg tgccgcgatg ccgacacccc cgtggccatc actctgggtg
       61 gaggctacgg tcagtcgctg caggataccg tggaagccca ctgcaacacc gtgcgagcgg
      121 ccaaggcagt cttcgacgac aaggttcccg gcctcaccta gcccttcttc tgccccccag
      181 ggtgtccgct gcctcagggt atccgtgctc gatggaagcg gcatcgctta tcagatggag
      241 tagtcctcgc ccctcgaggt cgcgaggtcc aaagccggcg gggtgtgccc gacctcgctc
      301 cccttgccgc cgcgattctc cagcgcgtcc acccgcgtca tcagggcccg gagcgccaac
      361 ccgatcgtat ccggcatcac cgtatgctcc aggtcggggg catcctgggc ggtgtgcggc
      421 ttgctccgcc ggacgatctg ccccggcaca ccgacgacga ccgaattggc cggcacggat
      481 ttcaccacca ccgcattggc tccgatccgg ctgtcctctc cgatggtgat cgcccccagg
      541 atcttggccc cggcaccgac caccacccga tctcgcagcg tgggatggcg cttgcccttg
      601 gcgaggctcg tccccccgag tgtcacgccg tgatacagcg tcacgtcagc gccgacctcc
      661 gccgtctcgc cgatgaccac gcccatgccg tggtcgatga agaagccggg gccgataacg
      721 gctcctggat gaatctcgat gccggtcaag gcccgagcca ggtgggatac ccatcgcgcc
      781 agcagcgtga acccgtgcct ccacagccag tgggacaccc ggtaccccca cacggcatgt
      841 agcccgggat agcagaacag gatctccgtc gcgccccgcg ccgccggatc ccgctccagc
      901 acagaccgta tgtctcgccg aagtgtctgg aacatgtttt cccccctcct gaggagaccg
      961 ccgcctgagc tcagcgatct tcgcctactc cgccaggtcc gcgaacaacg gggtactcag
     1021 gtatcgctcg ccgaacgacg ggatgatcac cacgacgagc ttcctggcgt tctcccggcg
     1081 ccgtgccacc tcgagcgcgg cccaggtggc ggccccggag gagatcccga ccagcaagcc
     1141 ctcctcccgt gccaggcgtc tggccgtcgt gaaggcatca tcgttcttca cccggatgat
     1201 ctcgtcgtag atcttcacgt tgaggatgtc cggcacgaat cccgcaccga tcccctgaat
     1261 cgcatgcggg cctttgggac cgccggagag caccgccgag gcctcgggct cgacggccac
     1321 gcatttgaag gatggcttgc gcgctttgag gacctcgccc acccccgtga tcgttccccc
     1381 cgtcccgacg ccagcgacca ggatgtcggc tgtgccgtcc gtgtcgcgcc agatctcttc
     1441 tgccgtggtc cgcctatgga cctcgggatt ggccggattc ttgaactgct gcgggatgaa
     1501 gtatcgggga tccgcggctg ccatctcctc tgccttccgg atggcgccgg ccatgccctg
     1561 gctggccggg gtcagcacca gttccgcccc gaacgcccgg agcagcatgc gccgctcttt
     1621 gctcatcgac tcgggcatgg tcagtacgca cttgtacccc cgcgccgcgc agacgaaggc
     1681 cagggcgatg ccggtattgc cgctggtcgg ctccaggatg atcgtgtccg gtcggatcgc
     1741 ctcgacctct tccgcggcct cgatcatcgc cacgccgata cggtccttga cgctgtgcgc
     1801 cggattgaag aactccagct tggcgacgac gtccgcgacg cagccctccg tcaggctctg
     1861 aaggcgcacc agcggagtgt tgccgacgag ttccgtgacg tcgtttgcaa tacgcatgtc
     1921 ttctctcctt catccggaaa aattcgagga tcaggccttg gtgatgacaa atacggccgt
     1981 ctcggcccca agagccggcc acacgcacac gcgatgctgg ccgcggagga agacacgccc
     2041 ctccgccttc agatccaata cccccaaggc ttcctggagc tgtcccaggg tgatggatcg
     2101 gacccgcggc tcgcgggacg cgagcgtcgt ccctgcgccg tccccgcaga gtgcacgcca
     2161 gcgcgccctc cagtacccgg cgttgtccag ggagaccacg gccgacctcc ccactcggag
     2221 catctctttg agaacggcca gagggcgatt caagtaagca agtgtctgac tcaggatgac
     2281 gaaatcgaac atgccctcag gaaagtccgc caggccgtcc tcgatattgc cgtggcggac
     2341 tgacagcccc ttggccacgc acgcccgcac attggcctcg tccacttcca cgccccgcgc
     2401 cagggcagcc ttgtgcgcct cgagctgcgc caggagcgtt ccatcgccgc agcccagatc
     2461 caggacgcgc gcaccttccg ggaccatttg aaggatgact tgcagatccg gccgggtagg
     2521 actcatgcgc tctcccgccg gcttgcgccg ttgaggctca ccggaccgcg ctgcttggcc
     2581 gaccatggcg ccaggggagc cacccgctct ccacctgtcg tggtgtccgg tcgaccggcc
     2641 gccgagcgca gagcggttgc tccgcgttcc agcttggcca agaagtcccg ggccagttcg
     2701 cccagccgat caacttccag caggaaggcg tcgtggccgt agtcgctctg aatctcgaca
     2761 taggtcgcgt cgatcccgtt ccgcaggagg gcccggacga gctcctcgga ctgttccggc
     2821 gggtagagcc aatcggagct gtagctgagg acgaggaact tcgcctggat tccagtcaac
     2881 gctgctgcca gatcggcttg ccccagagac agatcgaagt aatcgatggc cttggtgata
     2941 tacaggaagg agttggcgtc gaagcgcgcc gtgaaccgct tgccg
//
LOCUS       DBNGZC010000111         2667 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2258601, whole genome shotgun sequence.
ACCESSION   DBNGZC010000111 DBNGZC010000000
VERSION     DBNGZC010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2258601"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="AC16G0_02065"
     CDS             <1..216
                     /locus_tag="AC16G0_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098435.1"
                     /translation="IAHSLGIWIIALIQKILPMAKELPTLADPIGYLAILSIFIIITA
                     VARRVAFAILLVGWALILIRIAVMAGV"
     gene            693..1001
                     /locus_tag="AC16G0_02070"
     CDS             693..1001
                     /locus_tag="AC16G0_02070"
                     /inference="COORDINATES: protein motif:HMM:NF015102.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HIR5098436.1"
                     /translation="MRRHRKRGRHLSARPAADLRLLGVRHVYELVWMAPLDLQGQRNF
                     GARSLQEVQTKLSALGLRLGMSLDRPSYAAAVTAALVEEQFTQALTRVRALRGSKAAA
                     "
     gene            1019..1438
                     /locus_tag="AC16G0_02075"
     CDS             1019..1438
                     /locus_tag="AC16G0_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5098437.1"
                     /translation="MTRRRVLVVEDDAVIRKAMQKLLERSGYIYAGTGNGTQGLPLAA
                     RLHPDVILLDIRMPGLYSYEVCRRLKENPSTAAIPVIFLTAVHETSLERLAFDAGAVA
                     CITKPFRSGALLALIDAAIMSAELRAKHKRKGRGTGH"
     gene            1613..1987
                     /locus_tag="AC16G0_02080"
     CDS             1613..1987
                     /locus_tag="AC16G0_02080"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR5098438.1"
                     /translation="MVETPNRRRVARLAVPLHLGGLELELPLGRLLDLSAEGARIEFP
                     GHLHEGLVLFVELPPDLGGGRLTCEVVWTKLCKGGQTVEDDEHVYYYQSGLAFIGITP
                     EQQAALAVALAILKSGERPAGA"
     gene            2214..2348
                     /locus_tag="AC16G0_02085"
     CDS             2214..2348
                     /locus_tag="AC16G0_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
                     /protein_id="HIR5098439.1"
                     /translation="MTQTLDANGKLLTQLDPPGTTTYTWDARNRLTALSGPTVSASFA
                     "
     gene            2355..>2667
                     /locus_tag="AC16G0_02090"
     CDS             2355..>2667
                     /locus_tag="AC16G0_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098440.1"
                     /translation="MGRRTTKTINGRSTSFRYDGLDVTFEDGASGPASYLRTLAIDEA
                     LSRTESSGTLNYLTDALGSTLGLVDDAGALASTYTYAPFGNTAASGSSSANPFQFTGR
                     EN"
BASE COUNT          483 a          893 c          833 g          458 t
ORIGIN      
        1 attgcccact ccctcggcat ttggatcatc gcgctgatcc aaaagatcct ccccatggcg
       61 aaggagctgc cgacgctcgc cgatccgatc ggctacctcg cgatcctctc gatcttcatt
      121 atcatcacgg ctgtcgcgag gagggtggcc ttcgccatcc tcctcgtggg atgggcgctg
      181 atcctcatca ggatcgcggt catggcgggg gtctgagacg atgcctcgcg ccttctcccg
      241 cagctggccc ccttcccgcc cgaggaggtg acggcctatc ccgtcagtcc actcgtcaac
      301 aaccctgcga atgacactcc tgagtgccat gagccgatca cgttgtgaag atgtggtttg
      361 atcttcttcc aacattccca ctcgatcctc acgaccctct tgacgcaagg ccacctgttc
      421 gctagggtgt ctcttgagcc ttcctgctcc caccccacca cactgcggaa cggctcgccc
      481 agcccagtac cgaccgcgca gaatgggcca tgctggccct tgctccttag ctctcgtagt
      541 gcagcattcc ccttcgcatg tggccgacgg gtgcagcatg cccgagcgca gtcctcatct
      601 cctcacgcgc ggatccagcg acgggccgcc ctgggtccgc ctcgtcgtgc atccaatcga
      661 ggaggtctga accgccatcc tggtgggggc gcatgaggcg ccaccggaag cgcggtcgcc
      721 acctatccgc ccgccctgcg gcggatctcc gcttgcttgg cgtccggcac gtctacgaat
      781 tggtatggat ggcccccctg gacctgcaag ggcagcggaa cttcggcgca agatcgctcc
      841 aggaggtcca gaccaagctg tcggcccttg gactcaggct cgggatgagc ctcgaccggc
      901 catcctatgc ggcggcggtg acggccgccc tggtggagga gcagttcacg caggcgttga
      961 cccgggtgcg ggccctccgg ggctccaagg cagcagcgta gggcaaaggg ggaggaccat
     1021 gacgagaagg cgcgtgctcg ttgtggaaga cgatgccgtt atccgcaaag cgatgcagaa
     1081 gctactcgag aggtccggct atatttacgc cgggacgggc aatgggaccc agggcctccc
     1141 cctagcagcg cggctgcacc cggacgtgat cctgctggat attcgaatgc cgggactcta
     1201 tagctacgag gtctgtcggc gactgaagga gaatccgtcg accgcagcca ttccggttat
     1261 ctttctcacg gccgtccacg agacctcgtt ggaacggctc gcgtttgacg cgggtgccgt
     1321 cgcctgcatc acgaagccct tccgctccgg ggccctcctg gccctgatcg atgccgccat
     1381 catgagcgcg gaactccggg cgaaacacaa gaggaaaggc agagggactg gacactgacc
     1441 ctgccgggcg tggctgctct gaccggtgcc ggacgactac caggcacgcg aaccgggagc
     1501 acgccctggc gcggcgagag gagaacccga cgcacgccct ggcccaggtc caggctatcc
     1561 gcaatcggag gaccaggccg ggaccgaggc ccacaggggg gaggatcaga tcatggtcga
     1621 aacaccgaac cgtcgccgag tagcacggct cgctgtcccc ttgcatctcg gcggcctcga
     1681 attggaactc ccgctggggc ggctcctcga tctcagcgcc gagggggccc gcatcgagtt
     1741 ccctgggcac ctgcatgagg ggcttgtatt gttcgtagaa ctcccgcccg atcttggggg
     1801 aggtcgcctc acctgcgagg tcgtgtggac caaactctgc aagggcggac agaccgttga
     1861 agatgacgaa catgtctact actaccagag cggccttgcc ttcattggga tcacgcctga
     1921 gcagcaggcg gccctggcgg tcgcgctcgc aatcctcaag tccggggaac ggcctgccgg
     1981 agcgtaagcg gtcctgctga cccttcccga tagtctcgac gcacgaggct acatgctgga
     2041 ggcgatcaaa ctagtggggg cgaaggccag gaagacctgg gagagaagag cgcagccatg
     2101 acaccagtga tgggggacgc tccaattctc gtcagcttgc ggggagcggg ctcgagctgg
     2161 cggtcggggg agccgttcgt gaccaatggg actcatagat cgctttcggc ccggtgaccc
     2221 agaccctcga cgccaacgga aagctcctga cgcagttgga ccccccaggc accacgacct
     2281 acacctggga cgcccgaaac cgcctgaccg cgctcagcgg gccgacggtc agtgcgagct
     2341 tcgcctagga tgccatgggc cgccggacga ccaagaccat caatggccga agcaccagct
     2401 tccggtatga tggcctggat gtcacctttg aagacggcgc ttccggcccg gcctcgtacc
     2461 tccgcactct ggctatcgac gaagccctca gccggacgga aagctcgggg acgctcaact
     2521 acctcaccga cgccctcggg agcaccctgg ggctcgtgga tgatgccgga gccctcgcca
     2581 gcacttacac ctacgccccg ttcggcaaca ccgccgccag tggctcgtcg agtgcgaacc
     2641 cgtttcagtt caccgggcgg gagaatg
//
LOCUS       DBNGZC010000112         2651 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_107303, whole genome shotgun sequence.
ACCESSION   DBNGZC010000112 DBNGZC010000000
VERSION     DBNGZC010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_107303"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1618
                     /locus_tag="AC16G0_02095"
     CDS             <1..1618
                     /locus_tag="AC16G0_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012586313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098441.1"
                     /translation="AAALTAPTSALLLWLYRRAVLRAMAQAAGGGAPSTAANATLGGE
                     PRPLGISVIGSGERASMPAPAQSAFRDVSRRLRRAALVYLLAGLAYAIVLATPWMVFA
                     EGGFIPTRFLWLMSCYSWPIVLAVSLVAATSRRERLLVGGIYFAILACIGLWALVRNA
                     ELSAGQLGFFWLWENVPGTVLLLAFLQRRVRAVGPLVLVFMVAGVTGAFLAIGLAGSS
                     PALLRPIVAVGSFFGLGATALFVLLHLIGFAAFGLLGWWGLGRIGRRYRAKRTSDQAL
                     TLDAMWLLFAVAQSIEFAFEGWAWIFTGLVGFAAYKLVVRAGYSLTAAGSSGPMLLLL
                     RVFSLGHRSQRLLDALSKRWLRAGNIGLIAGPDLATATVEPHEFLEFVGGRLSRQFVQ
                     GEADLERRVAEMDRRPDPDGRHRVSEFFCHADTWQMTMRRLAGECDAVLMDLRSFSPA
                     NQGCRYELEQLLAHVPLDRVVLVVDESTDRAFLERALRDLWQRVPSDSPNRDLGQPEA
                     RLFEARGQSPAEIRTLLTLLFGASALKTAA"
     gene            1802..2134
                     /locus_tag="AC16G0_02100"
     CDS             1802..2134
                     /locus_tag="AC16G0_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010142788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098442.1"
                     /translation="MDLDTDGFIYRKWGAEQRCKRGDWLVDNEGDIYSVDSQVFAKTY
                     RKVGPGRFVKTTPVWAEVATGPGSVVTKEGRSHYTAGDYLVSNNEDGTDAYCMSAARF
                     GSMYEPDE"
BASE COUNT          435 a          835 c          862 g          519 t
ORIGIN      
        1 cgcggccgcg ctcaccgcgc ccacctcggc cctcctactg tggctgtatc gccgtgcagt
       61 cctgcgcgcc atggcccagg cggccggcgg tggtgccccg tcgaccgcag ccaacgcgac
      121 gttgggaggc gagccgcggc cgcttggcat ctcggtgatt ggctctgggg agcgggccag
      181 catgcccgct cccgcccaat cagcgtttcg ggacgtgtcc cgccggctgc ggcgcgccgc
      241 cttggtttac ttgctcgccg ggctcgccta cgcaatcgtc ctcgccacgc cgtggatggt
      301 ctttgccgag ggcggattca tccccacacg cttcctctgg ctcatgtcgt gctacagttg
      361 gcccattgtc ctcgcggtga gcctggtggc cgcgacgagc cgtcgtgagc gcctcctcgt
      421 cggggggatc tattttgcga tcctcgcctg cattgggctc tgggcactcg tccggaatgc
      481 cgagttgagc gcagggcagc tcggattctt ttggctctgg gaaaacgtgc ccggcaccgt
      541 gttgctgctg gcgtttctcc aacggcgggt gcgcgcggtc ggcccgctgg tgctcgtatt
      601 catggtggca ggtgtgacgg gcgcttttct cgccatcggc ctggccggaa gcagccccgc
      661 tctgcttcgt cccattgttg ccgtcggtag tttctttggc cttggggcaa ctgcgctgtt
      721 tgtccttctc cacctcatcg gcttcgccgc cttcggcctc ctcggctggt ggggacttgg
      781 ccggataggc cgccgctacc gggcgaagcg taccagcgac caggcgctca ccctcgacgc
      841 catgtggctc ctcttcgccg tggcacaatc gatcgagttc gccttcgagg gctgggcctg
      901 gatcttcacc gggctggtcg ggttcgctgc ctacaagctc gtagtccgcg cgggctattc
      961 gctcaccgcg gctggatcga gcgggccgat gctgcttctc ctgcgggtgt tctcgctcgg
     1021 gcatcgcagc cagcggctgc tggatgcgct ctccaagcga tggctgcgcg ccgggaatat
     1081 cgggctcatc gccggccccg acctcgccac cgccacggtg gagccccatg agttcctgga
     1141 gttcgtgggc ggccggcttt cccgccagtt cgtccagggg gaggcggatc tggagcggcg
     1201 cgtcgccgag atggaccgcc gtccggaccc cgacggccgt caccgggtga gcgagttctt
     1261 ctgccacgca gacacctggc agatgaccat gcgtcggttg gccggcgaat gcgacgcggt
     1321 cctcatggac ttgcgaagct tctcgcccgc gaatcaaggc tgccgctacg agctggagca
     1381 gctcctggcc cacgtgccgc tggatcgggt agttcttgtc gtggacgagt ccaccgaccg
     1441 ggcctttctc gagcgcgccc tgcgggacct gtggcaacgc gttccctccg actcgcccaa
     1501 cagggacctg ggacagcccg aagcccggct cttcgaagca cgcggccagt cgccggccga
     1561 gattcggacg ctgctgacgc tgcttttcgg tgcgagcgcc ttgaagaccg ccgcctgaag
     1621 gcagaatagg gacgtcttcg acagtggcag ccggtcgaat gttcaaaccg gtgaggacca
     1681 gaaaggtaga ctcgcagggg gatgttggcg tacgtctgcg tcccctggta aaatgccaga
     1741 cactatggca actcgccgcc gctacagaaa gaaggtcgac cgcttcgttg tcgccgttca
     1801 gttggacctg gacacggatg ggttcatcta tcgaaagtgg ggggccgaac aacgctgcaa
     1861 acgtggggac tggctggtcg acaacgaggg ggacatctac tcagtcgaca gccaagtctt
     1921 cgcaaagacc tatcgcaagg tgggccccgg ccgcttcgtc aagacgactc cagtctgggc
     1981 ggaggtcgca acgggacccg ggagtgtggt cacgaaagaa ggtcggtccc attacacggc
     2041 gggggactat cttgtctcca acaacgaaga cggcacggac gcctactgca tgagcgcagc
     2101 cagatttggc tccatgtatg aaccagacga atagaaggct ggtgaaaaag gctcatctgc
     2161 ggcgacccac cttacccgca agcccacctt tcaactgtac ctgaccctct tccccagtcg
     2221 gaatagataa acagatgctg tgggactcac ggtctcatgc gtctcgagac tgtgggcgcg
     2281 ctgacctttg agcatgtagc aggcgtcgga ggagggacag cggacagccc cagttgctac
     2341 agcggtgggt ggcggatcct tggggacccg agacggctga agtaccatca agtctaatgt
     2401 tgaaggatag atgcgccgaa acctgttgga gtatttttcc agggggaggc ctcggcataa
     2461 tcaatgcaca ccgagtaact cctgtatgag ctccaatgtc tctggcctct cctgcccaca
     2521 gttcgcatgc catcgagggt gactgaatcc acaatgggac ggggcaggtg gaccggacga
     2581 taaccggcac gtctgatagg agatcgccga gttcgactcc aggcgccaaa aagggctggg
     2641 aagttggtgt c
//
LOCUS       DBNGZC010000113         5843 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_889451, whole genome shotgun sequence.
ACCESSION   DBNGZC010000113 DBNGZC010000000
VERSION     DBNGZC010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_889451"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            389..4216
                     /locus_tag="AC16G0_02105"
     CDS             389..4216
                     /locus_tag="AC16G0_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5098443.1"
                     /translation="MGSPTRDELIAEVTQLRREVKSLTARQRELTALLQAHQTIFSSL
                     DLEQILQAIVEQAAAISGCASIRLFLLDETSQVLRFRVGFGPPAAELAALEVPTAESF
                     SGQVASTGTPLAVADTRGDPRVWRPDLASKYGHVSYLGLPVKLASRLFGVLAFSTPVP
                     RAYSGEEIALLSGFADRAALALENARLLHQERQRRRQLVALRAVSAEITRELDLPTLL
                     QLITQRAMELTGAAGGDVFLWDETSRLLTLQAAHGLAVGREKTTRRLGEGVMGTVAQR
                     RQGMLLNDYRSSAWAYPHALTPGEMTAVLVEPLLYHDRLLGVIGVNRVEGGQPFTQQD
                     RETLALFAVQAAIAIENARLHTAAVRRGAELEALLRGTQSIMSGLDLQGIFDRILAEA
                     AQITGCSHVKVLLVDKEAGRLRVGAIQGTAMSKQDRLPIGIGHSGIVAMTGQPLFSPD
                     APNDPRNAYAQQDRELGIVTYLGLPIKIRDEVRGVLSFNTTVPRQYTPAELAYLTSFA
                     DQAAIAIENARLHDAALRRGRELEALLRGTRSIMSGLDLQGIFDRIAAEAVRSTGCSH
                     VKLLLMDQEAGVLRVGSLLGTTLPPGFQIPLQGSLSGLVARTGQPVFRSEVSTDPENI
                     LAEQDRETGVVTYLGLPIKGRDEILGVLTFNTKEPRRYSSEELAYLTSFADHAAIAIE
                     NARLYEVVRRELDERSRAEKTLVARTQQLEAVRAISEELTRELDLPVLLELIIRRAEE
                     LVDASSGMVRLWDEVAQLLVPHAWTGMAAQRSTLRLQLGEGVAGTAAERREGLIVNDF
                     RTSQYASPRLLQDAAHTAVIAVPLLYRERLVGVLSIHRDQAGYPFTHTDQELLNLFAT
                     HAAIAIQNAHLFAELNQSYQRLRQAQEEMVRSEKLRALGQMAAGIAHDLNNMLAAILG
                     QVELLQFRVSDAEVQAGLAVLETAATDGAHVVRRLQDFARQRTHSSLKPLKLRPVIDE
                     ALAITRPRWEDEAQRRGAVITVQTHLDDCPPILGLAPEIREVLTNIILNAVDAMPQGG
                     TLTLTATLVRQPECTEAEGRGDQGEGAPQPDDPLASQPFVELSITDTGIGMTEAIRQR
                     IFEPFFTTKGGQGTGLGLSVAYGIMERHGGRIQVRSVPGQGTTVCLRFAVAPEETVAS
                     LGPRRTPASPQRVLLIEDDLTVRQTLASILQAAGHSVQEADGGAEGIDLLARVPVDLV
                     LTDLGMPGVTGWDVARAVRARTPRLPIILLTGWGEQAAVEAGAEGLIDRVLGKPVRMD
                     DLLQVIADLTSGG"
     gene            4370..>5843
                     /locus_tag="AC16G0_02110"
     CDS             4370..>5843
                     /locus_tag="AC16G0_02110"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5098444.1"
                     /translation="MAIRRLWQHIAPILRRYRRYFFLGILALLATDAIHLLIPWFTRL
                     ALDNLSAARKEWWGLLHYPALIVLAACGQGVFRYFWRTNVFGFSRNIEWDLRNVLFAH
                     LQRLPLSYFQHVKTGDLMSRLTNDLASVREMLGIGAVAALDGAVLILSALPLMIRIDP
                     WLTLWSLLSLPGITLLVLGFGNRIHRGYREIQQFLGKLSIFVQENLAGIRVVQAYAQE
                     ENQRQRFADLSAAYMRKNLVTARLSGMLWPLIAVFSGLGAATVLWLGGRRVLTGELTI
                     GQFVQFNGYLAVLTWPVMAIGYVVNLYQRGSSALVRLAEILETPVAPGSLMPGDGGSK
                     RLRGDIEFRGLSFRYGPEMPWVLRDVWLKIPAGTRCAIVGDVGAGKSTLVNLISRLFE
                     APEGGLFLDGHDVRTIPPPTLKASIGLVPQDIFLFSETIGENILFGRPDATVQDMEEA
                     ARIAALLPSIQEFSERFDTVLGERGVRLSGGQKQRTALARA"
BASE COUNT          940 a         1993 c         1857 g         1053 t
ORIGIN      
        1 ccctggtgga tcagcgggct ggagatgacc ttggtcgcgg tcatcggggg ggcggtgacc
       61 tatgcccttg gattggcgtt cgcgcttcac tgagccgctt tccacccgaa gggcccggtc
      121 cgaccacacc gccgcggaga gatctcttct ccccgacgac cggcgcccct cctggggtcc
      181 gcagacacgg cccttgacag cccatcgggc gccgcctaga cttcgctaca agttgaattc
      241 tgagcttgcg attcagcgct ctcctggcga tgagggacgg agccctccat actgagcctc
      301 agtgtcggcg gcgatcaggc cttgttcctc ggctggtccc gcgggtccat gacaaatgga
      361 acaagcctcg caaagtggga gcagtctcgt gggatcgccg accagggacg aactcatcgc
      421 agaggtcacc cagctgcgaa gagaagtcaa gagtctcacg gcccgccagc gcgaattgac
      481 cgccctcttg caggcccacc agaccatctt ctcctccctg gacctggagc agatccttca
      541 ggccatcgtc gagcaggcgg ctgccatctc cggctgcgcc agcattcgat tgttcctcct
      601 ggatgaaacc tcccaggtgc ttcgtttccg ggtgggtttt ggtccgccgg cagcggaact
      661 cgcggcgctc gaggtcccga ccgccgagag cttttccggc caggtcgcct ccacgggtac
      721 gccgctggcg gttgccgata ctcgcgggga tcctcgcgtc tggcgtcccg atctcgccag
      781 caagtacggc cacgtctcct atctcggcct cccggtgaag ctggcgtctc gactcttcgg
      841 ggtcttggca ttcagcaccc cggtgcctcg agcctactcg ggagaggaga tcgccctcct
      901 gagcggcttc gccgaccggg cggccctggc cttggagaat gcccggctcc ttcatcagga
      961 gcggcagcgg cgacggcaac tggtggcact ccgggcggtg agcgcggaga tcacccgaga
     1021 attggatctc cccaccctct tgcaactcat cacgcagcgg gccatggagc tcacgggggc
     1081 agcgggagga gatgtctttc tttgggatga gacgtcgcga ctcctcaccc tccaagccgc
     1141 ccacggtctg gctgttgggc gggagaagac cacccgccgg ctcggagagg gcgtgatggg
     1201 gaccgtggcc cagcgccggc aaggcatgct cctgaatgac tatcgctcct cggcctgggc
     1261 ctacccgcat gccctgacgc ctggggagat gaccgcggtc ctggttgagc ccctcctcta
     1321 tcacgatcgg cttctcggcg tgatcggtgt aaaccgagtc gaggggggtc agccctttac
     1381 ccagcaggac cgtgaaaccc tggccctctt tgccgttcag gctgccatcg ccatcgagaa
     1441 tgcccggctg cacaccgctg cggtgcgccg cggggcggaa ctggaagcac tccttcgcgg
     1501 cacacagtcg atcatgtcgg gcctggacct gcaggggatc ttcgatcgga tcttggcaga
     1561 ggccgcccag atcaccgggt gctcccacgt caaggtgctc ctcgtggaca aggaagcagg
     1621 acgactccgc gtcggggcga tccaagggac agccatgtcc aagcaggacc gtctgcccat
     1681 aggcatcggc cattccggga tcgtcgcaat gaccggtcaa cccctgttca gcccggatgc
     1741 gcccaacgat ccccggaatg cctacgccca acaggaccgg gagttgggga tcgtcaccta
     1801 cctcggcctt ccgatcaaga tccgggacga ggtgcgcggc gtcctgtcct tcaacaccac
     1861 ggtcccccgg cagtacacgc ccgccgaact cgcctacctg acctcctttg ccgatcaggc
     1921 ggccatcgcc atcgagaacg cccggctgca cgacgcggca cttcgccggg gaagggagct
     1981 ggaggccctc ctccgcggca cccggtcgat catgtcgggt ctggacctcc aggggatctt
     2041 cgatcgaatc gcggcagagg cggtccggag caccggatgt tcccacgtga agctccttct
     2101 gatggaccaa gaggccgggg tcctgcgtgt cgggtcgctc ctggggacca cgttgccgcc
     2161 cggcttccag atccccctcc aggggagcct gtcaggcctc gtggcgcgga ccgggcaacc
     2221 tgtgttccgg tcggaggttt cgaccgaccc tgagaacatc ttggccgaac aggatcgaga
     2281 gacgggtgtc gtcacctacc tcggcctccc gatcaagggt cgcgatgaga tcctgggtgt
     2341 gctcacgttc aacacgaagg aaccccgccg gtactcctcc gaggagttgg cttacctcac
     2401 ctccttcgcg gaccatgccg ccatcgccat cgagaatgcc cggttgtacg aggtggtcag
     2461 gcgggagctc gacgagcggt cgcgggcgga gaagaccctg gtggctcgta cccagcagct
     2521 cgaggcggtc cgcgccatca gcgaagagct cacgcgagag ctggaccttc ccgttctcct
     2581 ggagctcatc atccgtcggg cggaagagtt ggtagatgcc tcctccggga tggtccgcct
     2641 ctgggacgag gtggcccagc tcctcgttcc ccatgcctgg accggcatgg cggctcaacg
     2701 ctcgaccctt cgcctgcagc tcggagaggg ggtggccgga acggcggccg agcgacgcga
     2761 gggattgatc gtcaacgatt tccggacgtc gcagtatgcc tctccacgcc tcctccagga
     2821 cgccgcccac acggcagtca tcgccgtccc cctcttgtac cgcgagcgcc tggtcggcgt
     2881 cctctccatc catcgcgatc aggccgggta cccattcaca cacacagacc aggaactcct
     2941 caatctcttc gccacccacg ccgccatcgc catccaaaac gcccacctct tcgccgagct
     3001 gaatcagtcc taccagcgcc tgcgccaggc gcaggaggaa atggtccgct cggagaagct
     3061 gcgcgccctg ggccagatgg cggcgggcat tgcccatgac ctgaacaaca tgctggccgc
     3121 catcctcggc caggtggagc tcctccagtt tcgggtgtct gacgccgagg ttcaggccgg
     3181 actggccgtt ctggagaccg ccgccacgga tggcgcccac gttgtccgcc gcctgcagga
     3241 ttttgcccgc cagcggacgc actcctcttt gaaacccctg aagctccgtc ccgtgatcga
     3301 tgaggccctg gccatcaccc gccccagatg ggaggacgag gcgcagcgcc ggggggcggt
     3361 gatcacggtc cagacgcacc tggacgattg ccccccgatt ctcggcctgg ctccggagat
     3421 ccgcgaggtg ttgaccaata tcattctgaa tgcggtggac gccatgcccc aggggggcac
     3481 gctcacgttg actgccacac tggtccggca gccggagtgt accgaggcag aggggcgagg
     3541 cgaccaaggc gaaggtgctc cgcagcccga cgaccccttg gcttctcagc cattcgtgga
     3601 gctgtcgatc acggacaccg gcatcgggat gaccgaggca atccgtcaga ggatcttcga
     3661 acccttcttc accaccaagg gtggccaggg aaccggtctc ggcctctccg tcgcctatgg
     3721 catcatggag cgtcacggtg gccgcattca ggtgaggtcg gtgcccggtc agggcacgac
     3781 cgtctgcctg aggttcgctg tcgccccgga agagacggtc gcctcgctgg ggccgcggcg
     3841 tacgccggcc tcgccccagc gcgtcctctt gatcgaggac gacctcacgg ttcgtcagac
     3901 ccttgccagt atactccagg ctgcaggcca ttcggtgcag gaggcggatg gcggcgcgga
     3961 gggaatcgat cttctcgcca gggtcccggt ggacctggtc ctcaccgatc tcgggatgcc
     4021 tggggtaacc ggctgggatg tggcccgagc ggtgagggcc cgcacgccgc gcttgcccat
     4081 cattctcctc acgggatggg gggagcaagc agccgtggag gcaggtgccg aaggcttgat
     4141 tgatcgcgtc ctcggcaaac cggtgcggat ggatgacctt ctgcaggtga tcgcagacct
     4201 caccagcggt gggtagtcaa gccgtgaacg ccccgcctcg cctgcgactc ccgagcgtcc
     4261 ggatcgcctg atccatcctc cgccgccacg aaattcttgc aaactggcgc ctcacccatc
     4321 agaatttcgg ctcgtatctc catccacgac tattctatgg gtcaggaggg tggccatccg
     4381 taggctctgg cagcacatcg caccgattct tcgccgctat cggcggtact tcttcctggg
     4441 catcctggcc cttctggcta cggatgccat ccacctgttg atcccctggt tcacccggct
     4501 cgccctggat aatctgtcgg ccgcccggaa ggagtggtgg ggtctgcttc actaccccgc
     4561 gctgatcgtc ctggccgcct gcggccaggg agtcttccga tacttctggc ggaccaacgt
     4621 ctttggcttc tcgcgaaaca tcgagtggga tctccggaac gtcctctttg cccacctgca
     4681 acggcttccg ttgtcgtatt tccagcacgt gaagactggc gatctgatgt cccgcttgac
     4741 caacgacctg gcctcggtgc gggagatgct cgggatcggg gccgtggccg cgctggacgg
     4801 ggcggtgctg atcctctcgg cgctgcccct catgattcgg atcgacccct ggctcaccct
     4861 ctggtcactc ctctccctgc cgggcatcac cctcttggtc ctgggtttcg ggaaccgcat
     4921 tcaccgggga taccgggaga tacagcagtt cctggggaag ctgagcatct tcgtgcaaga
     4981 gaacctggcc ggcatccggg tggtccaggc ctatgcccag gaggagaacc agcgacagcg
     5041 attcgccgac ctgagcgcgg cctacatgcg caagaacctg gtgacggccc ggctcagcgg
     5101 gatgctgtgg ccgctcatcg cggtcttctc gggcctcggg gcggccaccg tcctctggct
     5161 gggcgggcgg cgggtgctga cgggggaact caccatcggc cagttcgtgc agttcaacgg
     5221 ctacctggcc gtcctgacct ggccggtgat ggccatcggc tacgtggtga acctctacca
     5281 gcgcggatcc tccgccctcg tccggctggc ggagatcctg gagacacctg tggccccggg
     5341 ctccctcatg cccggagacg gcggatcgaa gcggcttcgg ggagacatcg agttccgagg
     5401 cctctcgttc cggtacgggc cggagatgcc ctgggtgctg cgggacgtct ggttgaagat
     5461 tccagccggg acccggtgtg ccatcgtggg cgacgtgggt gcgggcaaga gcaccctggt
     5521 caacctgatc tcccgcctat tcgaggcccc cgagggaggc ctgttcctgg acgggcacga
     5581 cgtccggacc attcccccgc ccacgctcaa ggcgtccata ggcctcgtgc cccaggacat
     5641 cttcctcttc tccgagacca ttggtgagaa tatcctgttc ggccgcccgg acgccaccgt
     5701 ccaggacatg gaggaggccg cgcggatcgc cgccctgctc cccagcatcc aggaattcag
     5761 cgagcggttc gacacggtcc tgggagagcg gggtgtccgg ctgtccggcg gccagaagca
     5821 gcgcacagcc ctggcccgcg ccc
//
LOCUS       DBNGZC010000114         6717 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_107365, whole genome shotgun sequence.
ACCESSION   DBNGZC010000114 DBNGZC010000000
VERSION     DBNGZC010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6717)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6717)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6717
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_107365"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..617)
                     /locus_tag="AC16G0_02115"
     CDS             complement(96..617)
                     /locus_tag="AC16G0_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family A"
                     /protein_id="HIR5098445.1"
                     /translation="MPDTDRVEISIRDAVVGDGAALAVIYNQGIVDRVATLETEERTP
                     EERRAWLDARDPRHPVMVAVADGQVVGFGSLNVFNPRKAYDHVADFSIYVERAWRGKG
                     VGSRLLRAIIQRARHIGYHKLVLSAFPFNESGVALYKKFGFRVVGIYQEQGLLDGRWV
                     DTIIMEKLLEGSS"
     gene            886..1542
                     /locus_tag="AC16G0_02120"
     CDS             886..1542
                     /locus_tag="AC16G0_02120"
                     /inference="COORDINATES: protein motif:HMM:NF013982.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HIR5098446.1"
                     /translation="MGAVGVQSEGCTMWGVKSSDRDFYSAFSQHAAATARAAGLLVEL
                     FEHPEREPLAVQIEREEHEGDKIVHGVINALRGTWITPFDRQDIQNLISRQDDMLDLI
                     HASSKRVALFDIQAASDEALALTRYILKASETVKQAVSMLNDLKRSEEILKLCEVLNE
                     IENDADEVFRRSLAKLYKKGNDPLEVMKWREIYENLETALDRGEDVANQIESLVLEYS
                     "
     gene            1539..2552
                     /locus_tag="AC16G0_02125"
     CDS             1539..2552
                     /locus_tag="AC16G0_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005315 - phosphate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIR5098447.1"
                     /translation="MTFVVVGIVGLALVFDFINGFHDAANSIATVVSTRVLSPRVAVV
                     WAAFFNFVAFLVFQTKVASGIAKGVDPEVATLTIVAAGLVGAIVWDLLTWWWGLPTSS
                     SHALLGGLAGAAIVKVGGFEALDAAFFLKTAAFIVISPAIGLVLAVLVMNGLMYFLGD
                     RAPGPLDRVFRKLQLVSAAFYSLGHGGNDAQKTMGIIVAALAAAGLYQHAPSGTHGEI
                     PLWVVLACHAAMGLGTMSGGWRIVRTMGMRITRLAPTGGFCAESSGAASLFLATALGV
                     PVSTTHTITGAIVGTGSVRKFSAVRWGVAGRIVWAWILTVPGSAAIGGLAYLLAHAAG
                     IPG"
     gene            2635..3525
                     /locus_tag="AC16G0_02130"
     CDS             2635..3525
                     /locus_tag="AC16G0_02130"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR5098448.1"
                     /translation="MSVLQIVVLAAIQGLAELLPVSSSAHVILAQRLMGLDPAAPEMT
                     FLLVMLHTGTMFSVLVYFWARWKRWLLPAGANGDLPAGSTGMSFLGQLVVATGCTGVI
                     GLGLKIFIERVILEGTLGHPKAEVEELFRNLPLIAAALLAAGILIIAAGLWAAEPKAA
                     AFDMANSVMIGVVQGFCLPFRGFSRSGATISTALFCGIRRDLAEDFSFALAVLLTPPV
                     ILIELRRLLMSSASHGAAGPSLAAQILPGLLGMVASFLAGLVALRWLSSWLEKGRWRY
                     FGYYCVFFSVVVFVGHRLGL"
     gene            3547..3951
                     /locus_tag="AC16G0_02135"
     CDS             3547..3951
                     /locus_tag="AC16G0_02135"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HIR5098449.1"
                     /translation="MQAIEQTVEFPVPADRLFDAYLNPMEHARITGGEVTIGREAGAE
                     FKAFNGMITGRILVVVPKRLIVQTWRASHWKETELDSILVLVFELMPTGGRIRITHVN
                     VPDHDHQGVTEGWEKYYWAPWRAYLERKQSTG"
     gene            4057..5070
                     /locus_tag="AC16G0_02140"
     CDS             4057..5070
                     /locus_tag="AC16G0_02140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtaA/CmuA family methyltransferase"
                     /protein_id="HIR5098450.1"
                     /translation="MRPAPAMTSRERFLAGIRGERVDRTPLGSPTSVATVEQMELTGS
                     FFPKVHLDGTAMARLAAGAREVLGYDAIMPIFSVTQEAAALGCEMDWGSIDTMPVAKT
                     HPWSEPDQVHVPEDFLKRPPIRAALDAIQILRRDYGDQVAIIGKVMGPWTLSYHLHGL
                     QDFLMETLLEPGKVRAFLDRLKAVPLLFGTAQIAAGADVLCLADHATGDLVRGSMYRD
                     FLQPIHQDLVRELACPVVLHICGDTLDRIGYIADAGFAAFHFDSKVDAKDAAQAAGRM
                     PLVGNVNNPELLLRGTPEAVVQHARYAVDSGVRVLAPECAIPLRTPLENLKALHRAVR
                     QEG"
     gene            5379..6146
                     /locus_tag="AC16G0_02145"
     CDS             5379..6146
                     /locus_tag="AC16G0_02145"
                     /inference="COORDINATES: protein motif:HMM:NF024257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR5098451.1"
                     /translation="MAAQTPSGPLIFAGSGSNVPITRLLAEAFRLVHPEITIEIPASI
                     GSGGGIRAAADGAIAVGLISRPLKDVEQGWGLTVLPYAKTAVIVGVHPSVAEAGITFD
                     ELVLIFRGIKTRWQDGHPIVVLTREPGDSSLEVLERDIPGFLAASAESYRTKRWKTLY
                     TDQEMTATLAKTPYALGIADLGTVAAERLPIRALKLNGHIPSPEHVRSGSYPLIKTLA
                     FVFRRDRLPAGARTFLDFVRGPAGAKILQANGYVLEE"
     gene            6150..>6717
                     /locus_tag="AC16G0_02150"
     CDS             6150..>6717
                     /locus_tag="AC16G0_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098452.1"
                     /translation="MPSAGQRLQLRRQLARRLAPVTFAIVGLISLGIPAAYYMLEADD
                     VQNRATTEARELADRLGPLLVDVPALWKYQGPKYRQLLDEYIRHRQVASIQVRDASGE
                     LVPEYAFRMADPPGRWTAPVSFGTAPLFANNQRMGSVTLGISHMPLLWATLQLFVFST
                     AVGVSLALVAYLYPIKIVKRLEAHIEDLV"
BASE COUNT         1137 a         2091 c         2190 g         1299 t
ORIGIN      
        1 cggttggcat cctgcgaccc tctctcacag cacgacaact cactcaacac gcacgtcgga
       61 atcgaggacg tcccgctgcc caaggcccga caagctcagg acgagccctc caggagcttc
      121 tccatgatga tcgtatccac ccagcgtcca tcgagaaggc cctgctcctg gtagattccg
      181 acaacccgga agccgaactt cttgtacagg gccacaccgc tctcgttgaa gggaaaggcc
      241 gagaggacaa gcttgtgata accgatgtgg cgggcccgct ggatgatggc ccgcagcagg
      301 cggcttccca ctccctttcc ccgccaggcc cgctcgacgt agatggagaa gtccgcgacg
      361 tggtcgtaag ccttgcgggg attgaagacg ttcaggcttc cgaagcccac cacctgacca
      421 tctgccacag ccaccatgac tggatgccga gggtcccggg cgtcgagcca ggcccggcgc
      481 tcttccggcg tccgttcctc cgtttcgagc gtggcaaccc gatccacgat cccctggttg
      541 tagatgaccg ccagggcggc cccgtctccc acgacggcat cccggatgga gatttcgact
      601 ctgtccgtgt cgggcatggc gcctccgatt gaacacgtgg cggcgatcat tctattcagc
      661 ggcctcgggc tgtcaagctc gggctggggg cacggaggac gatggaaaac gcattcggcc
      721 accgggtggc agaacctgcg gacggcatgc ggcacgtatt cttcagttgc cttctccgtt
      781 catctgcgct atgtgtgagc tatccgccag gctgttcacc tgggaagagg ggggactctt
      841 ctgctgaacc cgccccccat ccatcccatc cacgcgacgg gaaacgtggg cgcagtgggg
      901 gtccagagcg agggctgcac tatgtggggt gtcaaatcgt ccgaccggga tttctattca
      961 gccttctccc aacacgcggc ggcgacggca cgtgccgcag gactgcttgt cgagctcttc
     1021 gagcaccccg agcgagagcc tctggccgtg cagatcgagc gggaggagca cgaaggcgac
     1081 aagatcgttc acggggttat caacgcgctg agggggacgt ggatcacccc cttcgatcgc
     1141 caggacatcc agaacctgat ctcccgtcag gacgacatgc tcgatctcat ccatgccagt
     1201 tccaagcgcg tcgcgctctt cgacattcag gccgcgtccg atgaggcgct ggcgctgacc
     1261 cggtacatcc tgaaggcctc cgagactgtc aagcaggcgg tcagcatgct caacgacttg
     1321 aagcggtcgg aggagatcct gaagctgtgc gaggtcctga atgagatcga aaacgacgcg
     1381 gacgaggtct ttcgtcgatc tctggccaag ctctacaaga agggaaatga cccgctcgag
     1441 gtgatgaagt ggcgtgagat ctacgagaat ctcgagacag cgctcgaccg gggcgaggat
     1501 gtcgccaacc agatcgagag cttggtcctg gagtattcat gacgtttgta gtggtcggca
     1561 tcgtcggtct ggcgttggtc ttcgacttca tcaatggttt tcacgatgcg gcgaactcca
     1621 ttgccaccgt ggtgtccacg cgcgtgttgt cgcctcgtgt cgcggtggtc tgggcagcat
     1681 tcttcaactt tgtggccttc ctggtctttc agaccaaggt agcctcgggc attgccaaag
     1741 gcgtggatcc cgaggtggca acgctgacga tcgtggctgc cgggctggtg ggggccatcg
     1801 tgtgggacct gctcacgtgg tggtggggat tgccgacctc ctcgtcgcac gcattgctcg
     1861 gcgggctggc aggcgcggct atcgtcaagg tggggggctt cgaggccctg gatgcggcgt
     1921 tcttcctgaa aaccgcggcg ttcatcgtga tttctcccgc catcggactg gttctggccg
     1981 tcctggtgat gaacggcctc atgtatttcc tgggggatcg cgcgcccggc cccctggatc
     2041 gagtgtttcg aaaactgcaa cttgtctcgg cggcgttcta ctcgctcggc cacggcggca
     2101 acgacgcgca gaagaccatg gggatcatcg tcgcggcgct ggctgcggca ggactctatc
     2161 agcacgcgcc atcgggcacg catggcgaga tccccctctg ggttgtcctg gcgtgccatg
     2221 cggcgatggg tcttgggacc atgagcgggg ggtggcggat cgtgcgcacc atggggatgc
     2281 gcatcaccag gctggcgccg acgggcggct tctgcgcaga gtcgagtggg gcggcgtcgc
     2341 tcttcctggc gacggcactg ggcgtgcccg tttccaccac ccacacgatc acgggggcga
     2401 tcgtggggac gggatccgtc cggaaattct ccgccgtccg ctggggtgta gccggacgta
     2461 tcgtgtgggc ctggatcctg acagtcccag gctctgcggc catcggcggg ctcgcgtatt
     2521 tgctggcgca cgcggccggg attcccgggt agattttcgc cgccccggcg tcctgcagga
     2581 tcagctcgca cctcgcgacc ccttcccctg acaattcacg gtgagattga cagcatgagt
     2641 gttcttcaga tcgtggtcct ggccgccatc caaggactgg cggagctctt gccggtctcc
     2701 agctcagcgc acgtgatcct ggcgcagcgc ctcatggggc tcgaccctgc agcccccgag
     2761 atgacgtttc tgctcgtgat gctccacacg gggaccatgt tttccgtcct ggtgtatttc
     2821 tgggcgcgct ggaagcgctg gctgttgccg gcgggggcga acggcgacct gccggcaggc
     2881 tcgacgggga tgagcttcct ggggcagctt gtcgtcgcga cggggtgcac gggcgtcatc
     2941 ggcctgggtc tcaagatctt catcgagcgc gtgatcctgg agggaaccct gggtcatcca
     3001 aaggccgaag tggaggaact gttccggaat ctcccgttga tcgccgcggc gctgctggcc
     3061 gcagggattc tgatcatcgc agcgggactc tgggccgccg agcccaaggc cgcggcattc
     3121 gacatggcca attccgtgat gatcggagtc gtccaggggt tctgtctgcc gtttcgcgga
     3181 ttctcccggt ccggggccac catttccacg gcgctgttct gcgggatccg gcgagacttg
     3241 gctgaggatt tcagcttcgc cctggcggtg ctgttgaccc ctccggtgat cctcatcgag
     3301 ctgcggaggc tcctcatgtc ctcggcgtcc cacggggcgg ccggcccgag cttggccgcg
     3361 cagatcctgc ccgggctgct ggggatggtc gccagctttc tcgcgggact ggttgcgctg
     3421 cgatggctct cgtcctggct cgagaagggt cgctggagat actttggcta ctactgcgtc
     3481 ttcttttccg tcgtggtctt cgtgggtcat cggctgggtc tctgagaata cgaaggggga
     3541 aggggaatgc aggccatcga gcagacggtg gaatttccag tgccggcgga tcgattattc
     3601 gacgcgtatc tcaatccaat ggagcacgcg cggataacgg gaggtgaggt caccatcggc
     3661 cgcgaggcag gggcggagtt caaggccttc aacggaatga tcaccggccg gattctggtg
     3721 gttgtgccga agcgcctgat cgtccagacg tggcgcgcct cgcactggaa agagacggag
     3781 ctggactcga tcctcgtttt ggtctttgag cttatgccga cgggcggtag gatcagaatc
     3841 acccacgtca atgttccgga ccacgatcac cagggcgtca cagaaggatg ggagaagtac
     3901 tactgggcac cctggagggc ctatctggag cggaaacaga gtacgggtta gcacgccgat
     3961 tctcctcccc gcattcattg gccctggagg aggaggcggt cgaggtgtgg ggaggggaca
     4021 ggctcccatg gaagacccgg tgatgaagtc ggtgcaatgc ggccagcccc ggccatgacg
     4081 tccagagagc gcttcctggc agggatccgc ggggagcggg tcgaccgcac gccgctgggg
     4141 agccccacct ccgttgccac cgtggagcag atggagttga ccgggagctt cttccccaag
     4201 gtgcacctgg atggaacagc catggccagg ctggcggcgg gcgcccgcga ggtcctgggc
     4261 tacgatgcga tcatgccaat cttcagcgtc acccaggagg ccgcagccct gggctgcgag
     4321 atggattggg gttccatcga cacgatgcct gtggcgaaaa cccatccctg gtccgagccc
     4381 gatcaggtgc acgtgcctga ggactttctc aagcgaccgc ccatccgggc cgccctggat
     4441 gccatccaga tattgcgccg cgactacggc gaccaggtcg ccatcatcgg caaggtcatg
     4501 ggcccgtgga cgctgtcgta tcacctccac ggtcttcaag acttcttgat ggagacgctg
     4561 ctggaacccg ggaaagtccg agccttcctc gacagactca aggcggttcc cctcctgttc
     4621 ggcacggccc aaatcgcggc gggtgcggat gtgctgtgtc tcgcagacca cgcgaccggc
     4681 gatctggtgc gcggcagcat gtatcgggac ttcctgcagc ccatccacca agacttggtt
     4741 cgggaactgg cctgccctgt cgtcttgcac atctgcggcg acaccctcga ccggatcggc
     4801 tacatcgcgg atgctggctt cgcggccttc catttcgatt ccaaagttga cgcgaaggac
     4861 gccgcccagg ccgcaggccg catgcccctg gtcggaaatg tcaacaaccc cgagctcctt
     4921 ctccggggaa ccccggaagc cgtggtgcag cacgcccgct atgccgttga ctccggcgtc
     4981 cgcgtcctcg cccccgaatg cgccatcccc ctccgcaccc cgttggaaaa cctgaaggcc
     5041 ctccatcgcg ccgtcaggca agaaggctag cccgcctcga gccgtctccc agtcttggcc
     5101 gtcggaagac cattgacgtg gccatttccc gtttgacttc ctgctcggca gaagggtaga
     5161 gtccttccgg tgtccgaggt tcctctgcgg gcggtctggg gggctgcatg tcgctcatga
     5221 ggattcgccc ggagacgcgc atatggcccc gtggagtgct ctgctccaaa gggctgcgct
     5281 ctttctggga cgggttggac gcgaggacgc tcgtgaatcc gagccgttgg atggtcgtcg
     5341 ggctgctcgt tcttctgggt gccctaccag gatatccgat ggctgcccag acaccttccg
     5401 gcccgctgat cttcgcaggg tcggggagca acgtgcccat cacccggctg ctggcagaag
     5461 cctttcgcct ggtccatccc gagataacga tcgagatccc ggcgagtatc ggatctgggg
     5521 gtggaatccg ggccgctgcg gacggggcca tcgccgtggg gctaatctcg cgtcctttga
     5581 aggacgtaga gcaaggatgg gggctcacag tcttgcccta cgccaagacg gcagtcatcg
     5641 tcggcgtgca ccccagcgtg gcggaggctg ggatcacatt cgatgagctg gtcttgatct
     5701 tcagggggat caagacgcgc tggcaagacg ggcacccgat cgtcgtcttg accagggagc
     5761 cgggggatag ctccttggag gttctggagc gtgatattcc ggggtttctg gcagcctctg
     5821 ccgagagcta ccggaccaag cggtggaaga ccctctacac agaccaggaa atgaccgcca
     5881 ctcttgccaa gacgccatac gccctcggta tcgcagacct gggaaccgtc gcggctgagc
     5941 ggttgcccat tcgggcgctg aaactcaatg ggcatatccc ctcgccggag cacgtgcgca
     6001 gcgggagcta tcccctcatc aagaccctgg cctttgtctt ccggcgggat cgactccctg
     6061 cgggagcccg gaccttcctg gacttcgtgc gcgggccggc gggagcgaag atcctccagg
     6121 ccaacgggta cgtgctggag gaatagacca tgccgagcgc gggacagagg ctccaactca
     6181 ggcggcaact ggcccggcgg ctggccccgg tgacgttcgc catcgtgggc ctgatcagcc
     6241 tcggcatccc cgcggcctat tacatgcttg aagcggatga cgtgcaaaac cgcgcgacca
     6301 cggaggcgcg agaactggct gaccggctcg gacccctgct cgtggatgtg cctgccctgt
     6361 ggaagtacca ggggccgaag taccgtcagc tcctggacga gtacatccgc cacaggcagg
     6421 tggccagcat ccaggtgcgg gacgcctcgg gtgaactggt cccggagtac gcattccgga
     6481 tggcggaccc gcccggcagg tggaccgccc cggtttcatt cggcactgcg cccctcttcg
     6541 caaacaacca gcggatggga agcgtcactc tcgggatctc ccatatgccc ctgttgtggg
     6601 caaccttgca gctcttcgtc ttctcaacgg ccgtaggcgt cagccttgcc ttggtggcgt
     6661 acctttaccc gatcaagatc gtcaaaagat tggaggccca catcgaggat ctggttg
//
LOCUS       DBNGZC010000115         6091 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1085266, whole genome shotgun sequence.
ACCESSION   DBNGZC010000115 DBNGZC010000000
VERSION     DBNGZC010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6091)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6091)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6091
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1085266"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /gene="rny"
                     /locus_tag="AC16G0_02155"
     CDS             complement(<1..1463)
                     /gene="rny"
                     /locus_tag="AC16G0_02155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIR5098453.1"
                     /translation="MWVIMALAVSVALIGLAIGLALERRRQNRLKQQAGDEAKRILRD
                     AEREVEARRKEAELEIKDKMLQARTEFEQESRARREETQSFERRLNQREEILDRKLEV
                     LDRRERDVIGREQEIRNRDTALGQQEARWHQLVEEAKRKLEQVAGLTAEAAKAQLVQS
                     LEQEAKLHAAARVKRVEDEANEMADERAKQVIGRAICRIASEYVAEAAVSVVDLPSDD
                     MKGRIIGREGRNIRALEKATGIDIIVDDTPETIILSGFDSIRREIARVAIQRLIQDGR
                     IHPTRIEETVEKVTKEVEKDIREAGEQACFELGITSLHPELVRMVGRLKYRFSYSQNQ
                     LAHTKEVATLAGLLAAEVGLDVKALKRAALLHDIGKAADQGTEISHALLSAELAKKYN
                     EDARVVNIIAAHHEEVPPQYPEAILLQAADTLSAARPGARREIMETYVKRLAKLEEIA
                     TSFKGVEKCFAIQAGREIRVVVESEQINDQAAFVMAR"
     gene            complement(1751..1940)
                     /gene="ssrS"
                     /locus_tag="AC16G0_02160"
     ncRNA           complement(1751..1940)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="AC16G0_02160"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(2029..2349)
                     /locus_tag="AC16G0_02165"
     CDS             complement(2029..2349)
                     /locus_tag="AC16G0_02165"
                     /inference="COORDINATES: protein motif:HMM:NF017016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0000921 - septin ring assembly [Evidence
                     IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR5098454.1"
                     /translation="MTEQRKIEVEIFGERYVLRASDAPGYLQQVAEYVDGKFREVAKE
                     SPALVPSKVAVQASLNIADELFKQGEAARRLEEAVLARIDGLVELLHRGSAAAASDQT
                     AAPR"
     gene            complement(2349..2642)
                     /locus_tag="AC16G0_02170"
     CDS             complement(2349..2642)
                     /locus_tag="AC16G0_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098455.1"
                     /translation="MDTVLERLQMLEGKVRETLANLSQVRGERESLEAKVSTLQAELR
                     SREQDFVALQAAQDRLQEEVQRLRSEREEVRSRVEGLLEEIGRLETVVQGVGS"
     gene            complement(2831..5305)
                     /gene="pheT"
                     /locus_tag="AC16G0_02175"
     CDS             complement(2831..5305)
                     /gene="pheT"
                     /locus_tag="AC16G0_02175"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR5098456.1"
                     /translation="MSWLKEYVDVPLPVAELAERLTLAGLEVSSIQTIGLPGSPLPWD
                     PERILVGQIQEVRPHPSADRLVLATVEYGAAQPKTVVTGAPNLRVGESGQKVAFALEG
                     AQHWDGYAATAQVATLKGRKVRGVFSDAMILSEKELGLSDDHTGVLILAASAPVGVPL
                     ATYLGDVVLDVEITPNMARCMSVLGVAREVAALTGGRVRPPSVEMQATGAPIDGQVVI
                     AISDPSLCARYAATLIVGVAIGPSPEWMRRRLRLAGVRPINNVVDVTNYVMLECGQPL
                     HAFDYDVLVRRAKGVPQITVRPARPGEILTTLDGVTRPLGLERLLITDTAGPIAVAGV
                     MGGAETEVTPATTRILLEAANFNFVSIRKTTQALKLPSEASARFGRGVPPSAAGPAAM
                     RATELMRTLGGGEIARGVVDCYPAPQAPSVVTLTTTEIRRILGMEMPRAEVERVLTSL
                     EFRCEPVGETGLLVTAPDHRLDIGTGVVGAADIIEEVARVTGYDRIPMTEIADTLPPQ
                     RDNLTVDLEERVRDLLVGAGLQEVITYRLTTLEREAALVPGQGQAAGRSYVTLANPIS
                     TDRVVMRQSLLPGLLEVVAQNARLRDRLWFFEVGPVFLPQDASPLPAEPRKLGIAMAG
                     SLAPASWQDTTPARTGFFELKGVLDSLRQGLHLQAGGLEPISHPSFVPGRTALLSVGG
                     REVGVLGEIHPDVRKAFDLPAGPICLAELDLDGLLARVSLTYRVPPVPRFPPALQDIA
                     LVVDEDISAAQVLETIRSSAGSLLAEVRLFDVYRGKQVPAGKKSLAFSLAFQAMDRTL
                     TDVEVEAEKHRILDAATAQLGARLRG"
     gene            complement(5348..>6091)
                     /gene="pheS"
                     /locus_tag="AC16G0_02180"
     CDS             complement(5348..>6091)
                     /gene="pheS"
                     /locus_tag="AC16G0_02180"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR5098457.1"
                     /translation="VTLPGQPAAMGRLHPSTQVLRRICQIWANMGFQIYRSPDVETDE
                     MNFELLNIPAHHPARDMWDTFYTTTDGILLRTHTSPGQIRAMREYCPEPIRVILPGMC
                     YRYEQITARSEIQFHQVEGLAVGRNVTMTDLKGTLVSFAQRMFGGNTRVRFRASHFPF
                     TEPSAEMDVECILCGGSGCGVCKHTGWLEILGCGMVHPTVLKNGGYDPEVYSGFAFGM
                     GPERITMLRHRIEDIRYFWANDLRFLEQF"
BASE COUNT         1010 a         2111 c         1874 g         1096 t
ORIGIN      
        1 tcgcgagcca tgacaaaagc cgcctggtcg ttgatctgct cgctctcgac caccacccgg
       61 atctctcgtc cggcctggat ggcgaagcac ttctccaccc ctttgaagga ggtggcgatc
      121 tcctccagct tggccagccg cttgacgtaa gtctccatga tctcacgacg ggcgccgggt
      181 cgcgcggcgg agagggtatc cgctgcctgg agcaggatgg cctccggata ctgcgggggg
      241 acctcctcgt gatgggcggc gatgatgttc accacccgcg cgtcctcgtt atacttcttg
      301 gccagctccg ccgagaggag ggcatgggag atctcggtgc cctggtccgc cgccttgccg
      361 atgtcgtgca gcagcgcggc ccgcttcagc gccttgacgt ccagacccac ctctgccgcc
      421 aggagacccg ccagggtggc gacctccttg gtgtgggcga gctggttctg ggagtagctg
      481 aaccggtact tgagtcgtcc caccatccgc accagctcag ggtgaagact ggtgatcccc
      541 agctcgaagc aggcttgctc gcctgcctct cggatgtcct tctcgacctc tttcgtcacc
      601 ttctcgactg tctcttcgat gcgggtcggg tgaatccgcc cgtcctggat cagccgctgg
      661 atggccacgc gagcgatctc acggcggata gagtcgaacc ccgagaggat gatggtctcg
      721 ggcgtgtcat ccacgatgat atcgatgcct gtcgccttct ccagcgcgcg gatgttccgc
      781 ccctcgcggc cgatgatccg ccccttcatg tcgtcgctgg ggaggtccac gacggatact
      841 gccgcctcgg ccacgtactc ggaggcgatc cggcagatgg cccgtccgat gacctgcttg
      901 gcccgctcat ccgccatctc attggcctcg tcctcaaccc gcttgacacg ggcggcggca
      961 tgcagcttcg cctcctgctc cagggactgc acgagctgcg ccttggccgc ctccgccgtg
     1021 agccctgcca cctgctccag cttgcgtttg gcctcctcga ccagttgatg ccagcgcgcc
     1081 tcctgctggc ccagggccgt atcccggttg cggatttcct gctcccgtcc gatcacgtcg
     1141 cgctcgcggc gatccaggac ctccaacttg cggtccagaa tctcctcacg ttgattcaag
     1201 cgccgctcga agctctgtgt ctcctcgcgc cgcgcccggc tctcctgctc aaactccgtc
     1261 cgggcctgga gcatcttgtc cttgatctcc agttcggctt ccttgcggcg cgcttccacc
     1321 tcacgctcgg cgtcccgaag gatccgcttg gcctcgtctc cagcctgctg cttcagtcga
     1381 ttttgccgcc gtcgttcaag ggccagtccg atggccaagc ctatcagggc cacggacacg
     1441 gccagagcca ttatcaccca catatccacg gaggtccctc cctcatccgc gtgccttcac
     1501 agagtcggct tcggcaccct gggccgaccc gtcaccactc atgtgagccc tggacatgtc
     1561 tccggaggta tccgagccgg gctcgggcat cggtcccgca gccggaggcc acgtgcgtcc
     1621 accactgcca gatcgtcacg atcaccgcag aaggctacaa acagagtgcg cagcagggaa
     1681 cagagagagc gagtccgagg cgaggctaaa cggccggagc gggcaagaag atcaggcgtg
     1741 ccggaataat agaaccccta ccattgtgcc gtgtgtcggt ctttagttga acctggtaaa
     1801 ccaggtggag ttcccggaaa cgctttaggc ttcccttccg ccgaagcggg caggcctgcg
     1861 caccgcgttc tcgatgggag ctccgttcta ggggtgttgg ctcaaaaaaa gtcaccgcat
     1921 cacgtgcacg gtaggggctc tcggcattcc ttaccgctga tggccacgct agcacaggac
     1981 gtccctcgct gcaagccctt tctgaccctc gccgcacctt cccggtgctc accgcggtgc
     2041 tgcggtctgg tccgacgctg cggctgcgct gccgcgatgc agcaactcca ccagcccgtc
     2101 tatccgcgcg aggactgctt cctcaagccg gcgagcagct tctccctgct tgaacaattc
     2161 atcggcgatg ttgagtgagg cctgcaccgc cacctttgaa ggaaccagag cagggctctc
     2221 cttcgccacc tctcggaact tgccgtctac gtactccgca acctgctgga ggtaccccgg
     2281 cgcatccgac gcccgcagca cgtagcgttc accgaagatc tcgacctcga tcttccgttg
     2341 ctctgtcatc agcttccgac cccctggaca acggtctcca gtctgccgat ctcctccagc
     2401 agtccctcca cccgagaccg gacctcctcc cgctccgagc gcaaccgctg gacttcttcc
     2461 tgaaggcggt cctgagccgc ttgcagggcg acaaaatctt gctcgcggga acgtagctct
     2521 gcctgtagcg tcgagacctt ggcctccagg ctctcgcgct cgccccggac ctgggacaga
     2581 ttcgccaggg tctcgcgcac cttcccttcg agcatctgaa gtcgttccaa caccgtgtcc
     2641 atgcaatcac cccacgtatg ttgtctcaac ctagcaaagc cctaggcctt tgtaaagaag
     2701 gatttcaggg tgcgtcgctc ctggttcgcg ggtgaggctt cggggagtgc gcacgtggga
     2761 tggacggttc gggcaccgcc gattcgtggc ggacggccac gggaatcgac ccgccggata
     2821 gggtcaaaac tcacccccgc aggcgagccc cgagttgcgc agtcgccgcg tcgagtatgc
     2881 ggtgcttctc ggcctccacc tctacgtcgg tcagggtgcg atccatcgcc tggaatgcga
     2941 gcgagaaggc cagactcttc ttgcccgccg ggacctgctt tccccgatag acgtcgaaga
     3001 gacggacctc cgccagcagt gagcctgcgc tggaacggat cgtctccagc acctgggccg
     3061 cggatatgtc ttcgtcaacc acgagtgcga tgtcctgcaa agccggtggg aaccggggga
     3121 ctggcgggac gcgataggtg agcgagactc gcgccaggag cccgtccaga tccagttccg
     3181 ccaggcagat cggtccggcg ggaagatcga aggcctttct cacatccgga tggatctcac
     3241 ccaggacccc gacctcgcgc ccccccaccg acaggagcgc ggtccgtccg ggcacgaagc
     3301 tgggatgcga gatcggttcc aggccgccgg cttgaaggtg gaggccctgt cggagggaat
     3361 ccagcactcc cttcaactcg aagaaacccg tgcgggccgg agtcgtgtcc tgccaggagg
     3421 ccggtgccag agaaccggcc atagcgatcc cgagtttccg tggctcggcg ggcaacggac
     3481 tggcgtcctg agggaggaac accggcccca cctcaaagaa ccacagacga tcgcggagcc
     3541 gtgcgttctg cgccacgacc tccaacagac cggggagcaa gctctgacgc atgaccacgc
     3601 gatcggtgct gatgggattc gccagcgtga cataggagcg gcccgccgct tgaccttggc
     3661 ccggcaccaa tgccgcttcc cgctccaggg tggtcaggcg gtaggtgatg acctcctgca
     3721 agccggcccc caccagcaga tccctcaccc gctcctccag gtccaccgtc agattgtccc
     3781 gttgcggcgg gagcgtatcc gcaatctccg tcatcgggat ccggtcgtaa cccgtgacgc
     3841 gcgccacctc ttcgatgatg tcggcggctc ccaccacgcc ggtcccaata tccagtctgt
     3901 ggtcgggagc ggttaccagc agccccgtct cccccacggg ctcgcaacgg aactccagcg
     3961 aggttagaac tcgctcgacc tcggcacgcg gcatctccat ccccagaatg cgtcggatct
     4021 cggtggtcgt cagggtcacg acggatggcg cctgaggggc agggtagcag tccacgacac
     4081 cccgggcgat ctcccccccg cccaacgtac gcatcagctc ggtggcccgc atggcggccg
     4141 gccccgctgc cgacgggggg acgccccggc caaatcgggc gctggcctcg ctggggagct
     4201 tgagtgcctg ggtggtcttc cggatggaga cgaagttgaa gttggcggcc tccagcagga
     4261 tccgggtggt ggctggggtg acctccgtct cggccccgcc catgacgccg gccaccgcga
     4321 tgggccccgc cgtgtccgtg atcaggaggc gctcgaggcc gagagggcgt gtcaccccat
     4381 ccagggtggt caagatctcc cctggccggg ccgggcgcac cgtgatctgc ggcacccctt
     4441 tggcgcggcg caccagcacg tcgtagtcga aggcgtggag cggctgcccg cactccagca
     4501 tgacgtagtt ggtgacgtcc accacattgt tgatgggacg gacccccgcc aggcgaaggc
     4561 gccggcgcat ccactcgggc gagggaccga tggcgacacc cacgataagc gttgccgcgt
     4621 agcgcgcgca gagcgaggga tcgctgatgg cgataacgac ctgtccatcg atgggcgcgc
     4681 cggtagcctg catctccacg ctgggggggc ggactcggcc ccccgtcagg gcggccacct
     4741 cccgggcgac gcctagcacg gacatgcacc gggccatgtt gggtgtgatc tccacatcca
     4801 ggaccacgtc ccccagatac gtggccaggg gaacccccac cggggcggac gcagccagga
     4861 tcagcacgcc ggtgtgatcg tccgagaggc ccagctcctt ctccgagagg atcatggcgt
     4921 ccgagaagac gccgcggacc tttcgtccct tgagcgtggc gacttgcgca gtggccgcgt
     4981 atccatccca gtgctgagcc ccttccagcg cgaaggccac cttctgcccg gactcaccca
     5041 cccgcagatt gggggccccc gtcaccaccg tcttgggctg ggccgccccg tactccacgg
     5101 tcgccagaac aaggcgatcc gcactggggt gcgggcggac ctcttggatc tgtcccacca
     5161 ggatccgctc cggatcccaa ggcagcgggc tgcctggcag accgatcgtt tggatgctcg
     5221 acacctccaa cccggcaagg gtcagccgct ccgccagctc tgccaccggc agaggaacgt
     5281 cgacatactc cttcagccaa ctcatcggca cgcgcatgga tgtcaacccc gtacttggac
     5341 ggggtcctca gaactgttcc aggaaacgga ggtcgttcgc ccagaagtag cggatatcct
     5401 cgatccggtg ccgcagcatg gtaatgcgtt cgggacccat gccgaaggca aaccccgaat
     5461 agacttcggg gtcatagcct ccgttcttga gcaccgtggg gtgcaccata ccgcacccga
     5521 ggatctccag ccagccggtg tgcttgcaca ccccgcagcc gctgccccca catagaatgc
     5581 actccacgtc catctcggcg ctgggctcgg taaacgggaa gtggctggca cggaatcgca
     5641 ctcgcgtgtt gccgccaaac atacgctggg cgaagctgac gagggtgccc ttcaggtcgg
     5701 tcatggtcac gttccgtccc accgctagcc cctcgacttg gtggaactgg atctccgacc
     5761 gggcggtgat ctgctcgtag cggtagcaca tccccggcaa gatgacgcgg atcggctccg
     5821 gacagtactc gcgcatggcg cggatctgcc ccggcgaggt gtgggtccgt aggaggatgc
     5881 catccgtcgt ggtgtagaat gtgtcccaca tgtcccgggc cggatggtgg gccgggatgt
     5941 tcagcagctc gaagttcatc tcgtcggttt cgacgtccgg gctgcggtag atctgaaacc
     6001 ccatgttcgc ccagatctgg cagatgcgcc tcaggacctg cgtgctcgga tgcaggcggc
     6061 ccatggccgc gggttgcccg ggcagcgtga c
//
LOCUS       DBNGZC010000116         8418 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_498170, whole genome shotgun sequence.
ACCESSION   DBNGZC010000116 DBNGZC010000000
VERSION     DBNGZC010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 8418)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8418)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8418
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_498170"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="AC16G0_02185"
     CDS             complement(<1..865)
                     /locus_tag="AC16G0_02185"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5098458.1"
                     /translation="MRILVTGGAGFIGSHLVDALAARGDTVVVYDNLAQGQSENLAAV
                     QGRVVVRDGSVVDLDLLERTVREHLVDKIVHVAAIASRDVLDPSLIISVNIQGTVNVL
                     EAARRCEVRRCVLLSSDEVYGEFQYEPANENHPLVPRHPYGISKLCGERFAEYYHEFH
                     GVDAVSIRTARVYGPRFLGERVPKNLIQNALRGKATRMPKGGDDKADYTYVRDAVRGI
                     LMALDAEKPRYYVYNITGGKAYTTTQVGQIIQGMFPSAEVDIGPGPVEVDGVRLPMKG
                     TMDITRARDDLG"
     gene            complement(1004..1714)
                     /locus_tag="AC16G0_02190"
     CDS             complement(1004..1714)
                     /locus_tag="AC16G0_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5098459.1"
                     /translation="MAVLEVEGIDVFYGDLQALQHVTLRVGEGEIVALVGSNGAGKST
                     TLRTISGLIRPRAGTIRYDGNMIDRLPAAEIARRGIGHVPEGRRLFPMMTVWENLQIG
                     ACTPDAKRRFDETLTVIFGLFTRLKDRRHQLAGTLSGGEQQMLAIGRALAMRPRLLMM
                     DEPSLGLAPKLAQEIFATVRNINTQGVTVLLVEQNVKQALRLAHRAYVLENGKLILEG
                     KSSDLAQNEHIHKAYLGI"
     gene            complement(1701..3512)
                     /locus_tag="AC16G0_02195"
     CDS             complement(1701..3512)
                     /locus_tag="AC16G0_02195"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HIR5098460.1"
                     /translation="MGRTDRQSQVGVLGAFGPRGIAIGLLLLVFLLLPLFVRSPYHLH
                     LVILFLLWVGLGQSWNLLGGFTGQVSFGHAAFFGTGAYVTSLLYVGPGIPLVVGGLLG
                     GLFAAVLSIPIGLICFRLRGPYFALTILGISEILRIIAINWRSLTKGPVGVLFPPIFR
                     DRLPFYYIVLALAVLTMVVVWLTLRSKAGYYFRALRDDQDAATALGIDPTRFKLVSLI
                     LSAFLTGLLGGFFAPYQGYIDPDVVFSIPDISIAMIVVVVLGGMGNLWGPPVGAIIVV
                     VLSEIFRATLGEAHLLVYAALMIVIVVFLPEGLVGWFARIFSQWSSHRRPAVSTLPDW
                     PAATVSPEPGPAGRALLAVGGDGGPDGVLLRAEKVTKAFGGLLALQSVDFQVRQGEVL
                     GLIGPNGAGKTTLLSVISGFEQATAGKITFGSHDITRLPAYRAAHLGVARTFQIVRPL
                     KKMTVLDNVVTAALANTVRVGAARQKAEEILQFVDLYHRRQVLAAGLTLGERKRLEMA
                     RALATSPKLLLLDEVAAGLNSTETKAVVAILERIREAGISIVAVEHVMQVIMSISDRI
                     VVLDYGQVLAEGKPAEVARNPEVIQAYLGASEANGSA"
     gene            complement(3543..4421)
                     /locus_tag="AC16G0_02200"
     CDS             complement(3543..4421)
                     /locus_tag="AC16G0_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5098461.1"
                     /translation="MGSQVFLQSVLNGILLGGLYALIGAGMSLIFGVLRVINLAHGEM
                     LMVGMYVTFWLFTLFGVNPYVSVLISFPVLFLLGAVTQQFLVGPLLRRNAPEENQLLL
                     TMGVGLGLTEVVRLLFTANYRTFSTPFATATLTVLGISVSLALLSAFAIALVISLLLY
                     SVLLKTDLGRSLRATAQDKDAASLYGIDGDRVYTLAFGIGAGLAGAAGSLLLPIFYLS
                     PTVGGSFTMKAFIVTVLGGMGSVLGALVGGLTLGIAESLGAVYVSTAYRDVIGYLVFI
                     IVLSLRPSGLVGRSRF"
     gene            complement(4553..5794)
                     /locus_tag="AC16G0_02205"
     CDS             complement(4553..5794)
                     /locus_tag="AC16G0_02205"
                     /inference="COORDINATES: protein motif:HMM:NF024825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098462.1"
                     /translation="MMIRRKRCPGRTFRPALWLLASCLVLVTLLPGVAVQAAQFKGSI
                     KVGVPLPLTGRHAPFGEIIKNSFVLAADEVNAKGGVRGGYKLDLVFEDSRSDVQAART
                     VTEKLINQDKVVMLTGQYSSVETFPMAQMVQQYGIPFLCSTAAADQITEQGWKYIFRM
                     VQPASEFDDGLKDFFEKVAKPKSIAILFENTLFGTSTAAAMKEWATKNRDEAVMYEPY
                     DAGAPDYRPLLLRVKEKKADVIYMVSYLLDAVLLMRQAKEVDLNPQAFTGAAAGFSIL
                     PFIKGAGDVAENVLSSTMWEASVPYPGAANYLKDYIARFGSPPSYHGAQGYAAVFVVA
                     DVLNRAASLKPDDIVESLSKTDMMTMYGRIRFESYKKFQNQTKLPTFVIQVQKGKFVT
                     VWPKDVATAPAAYPTPPWSKR"
     gene            complement(5996..6835)
                     /locus_tag="AC16G0_02210"
     CDS             complement(5996..6835)
                     /locus_tag="AC16G0_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR5098463.1"
                     /translation="MNEHSLKKDRNQAIGGPIIQSLQRGLGILEIIAKKGKGITMAEV
                     SREIGLHTSTTFHLLRTLSTLGYLVQDETTREYLLGSKVFHLAASAYTEAQISKIAKP
                     FLVEMARQTGEASHMAILERGEVIVIDKIDGSSPVRVSDRVGYPRPAHCTAIGKTLLA
                     YLPEAELKTFLEASDFRPFTAKTITAAPILEQELKRVRKQGYAFDDEEFAQGIRCLAA
                     PVRNFTGHVVAAIGISGPVWRVSLDRVAQLTEFVKTMGHRLSQQLGHSGEEGGDGEDP
                     PKP"
     gene            7128..>8418
                     /locus_tag="AC16G0_02215"
     CDS             7128..>8418
                     /locus_tag="AC16G0_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR5098464.1"
                     /translation="MDLWREFHGPNAGYVLELYDRYRQNPASVDEATRAFFARWTPPA
                     TGTPGSPAPACETIVGAVNLAQSIRFYGHLAARLDPLGSPPRGDPSLLPETYGITEAD
                     LQRLPAHLIGGPVAERAANAHEAIQALRAVYSSTAGYGYGHIQAPEERDWLRHAAESG
                     CFRSPQDPINHQNLLQRLTQVEAFEHFLHRIFPGKTRFSLEGLDMLVPILDEIIGEAA
                     ETETRSILIGMAHRGRLNVLAHVLNRPYAQILAEFKDPTRGRNFTIREDLSWTGDVRY
                     HLGAHRAIEDGMPVDVVVSMPPNPSHLEYVNPVVEGMARAAGTRADGPGPPLFDPHIS
                     LPILIHGDAAFPGQGVVAETLNLSRLPGYHTGGTIHIIANNQLGYTTPPEDERSTLYA
                     SDLAKGFEIPIVHVNADDPEACIEAARLAFAYRARFHK"
BASE COUNT         1611 a         2740 c         2534 g         1533 t
ORIGIN      
        1 agccgagatc atccctggcc ctggtgatgt ccatggttcc cttcatgggg agcctgaccc
       61 cgtctacctc cactgggccg gggccgatgt ccacctccgc tgacgggaac atcccctgga
      121 tgatctgtcc cacctgggtg gttgtatagg ctttaccccc ggtgatgttg tagacgtaat
      181 accgtggttt ctcggcatcc agtgccatca ggattccccg gacggcgtcc cgcacgtagg
      241 tatagtccgc cttgtcatcc ccgcccttcg gcattcgagt cgctttcccc cgcagggcgt
      301 tctggatcag attcttcggg acgcgctccc cgaggaatct cgggccatag acccgagccg
      361 tccggatgga taccgcgtcc acaccatgga attcgtggta gtactcggcg aagcgttcgc
      421 cacacagctt gctgatgccg taggggtgcc gggggaccag tgggtgattc tcattagccg
      481 gctcgtactg aaactcgccg tagacctcat ccgagctgag gagcacgcat cggcgaacct
      541 cgcatctcct cgccgcctcc aggacattta ccgtcccctg gatattgaca ctgatgatga
      601 gacttgggtc gaggacatcg cgagaggcaa tggctgcaac atggacgatc ttgtctacga
      661 ggtgctcgcg caccgttctt tcgagtaggt cgaggtcgac gacgctgcca tcccgcacta
      721 ccacgcggcc ctggacggcc gccaggttct ccgactggcc ttgagccagg ttatcataga
      781 caacgaccgt gtcccccctg gccgccagcg cgtccacgag atgggagccg atgaaccccg
      841 cgcccccggt gacgagaatc cgcatcagcg tccccgtttc cagatcactc ccgccattac
      901 gcgagcggcc tggcacacca acgatagaca gtggcaacac cgtctgtgcc gatcgctgcc
      961 tgaattccac tgcccccgag aggtctcatt ccgatcccac cactcagatg ccgagatagg
     1021 ctttatggat atgctcattc tgggcgaggt cggaggactt gccttcgagg atgagtttcc
     1081 cgttttcgag gacgtaggcc cggtgcgcga gcctcagggc ctgcttcacg ttctgctcca
     1141 ccagcaggac ggtcacgccc tgggtgttga tgttccggac cgtcgcaaag atctcctgcg
     1201 ccagcttggg tgccagtccg agcgaaggct cgtccatcat caggagtcgc gggcgcattg
     1261 ccagagcccg gccgatagcc agcatctgct gctcaccgcc tgacagcgtg ccggcgagct
     1321 ggtgccggcg atccttgagc cgggtgaaga gtccgaagat gacggtcagc gtctcgtcga
     1381 atctgcgctt cgcgtcgggt gtgcaggcgc caatctgcag gttttcccac accgtcatca
     1441 tggggaagag gcgccggcct tcgggaacgt ggccgatgcc gcgtctggcg atctcagcgg
     1501 cggggaggcg atctatcatg ttcccgtcgt agcggatggt ccccgcgcgt ggccgtatca
     1561 ggccggaaat ggtcctgagg gttgtgctct tccccgcgcc gttggaaccc acgagcgcga
     1621 cgatctcccc ttccccaacc ctcagcgtga cgtgctgcag ggcctgcagg tccccataga
     1681 aaacgtcgat tccctcgacc tcaagcactg ccattcgcct ccgacgcacc aaggtacgcc
     1741 tggatcactt cggggtttcg ggccacctcg gctggtttgc cctcggcgag aacctgaccg
     1801 tagtcgagta ccacgatgcg atccgaaatg gacatgatca cctgcatcac gtgctccacc
     1861 gccacgatcg agatcccggc ctcccggatc cgctccagaa tggcgactac tgccttggtc
     1921 tcggtggagt tgagacccgc cgccacttcg tcgagcagca ggagcttggg gctcgttgcc
     1981 agcgcgcggg ccatctcgag gcgtttccgc tctcccaggg tcaggccggc cgccagcacc
     2041 tggcggcggt ggtacaggtc gacaaactgg agaatctcct cggccttctg ccgggcagca
     2101 cccacgcgga cggtgttggc cagcgccgcc gtcaccacgt tgtccagcac ggtcatcttc
     2161 ttgagtggcc gcacgatctg gaaggtgcgc gccacaccaa ggtgcgcggc ccgatacgct
     2221 ggcagtcggg tgatgtcatg ggagccgaag gtgatcttac ccgcggttgc ctgttcgaac
     2281 ccggagatca cggacagcag ggtggtcttt ccggccccgt tgggtccgat cagacccagg
     2341 acctcaccct gccggacctg gaaatccacg ctttgaagcg cgagcagccc cccgaaggct
     2401 ttcgtgacct tctccgcgcg cagcagcacg ccgtccggcc ctccatcccc gcccaccgcg
     2461 aggagcgccc gccccgctgg accgggttcc ggggaaacgg tcgcggctgg ccaatccgga
     2521 agggtggaga ctgcaggtct acggtgagac gaccactggg aaaagatccg ggcgaaccag
     2581 ccgaccagcc cttccggcag gaagacgacg atgacgatca tgagcgcggc gtagaccagc
     2641 aggtgcgcct cgcccagggt ggcacgaaag atctcggaga gcaccaccac gatgatcgcc
     2701 ccgacgggtg gaccccagag atttcccatg cccccgagga caaccacgac aatcatggca
     2761 atggagatgt cgggaatgga gaagaccacg tcggggtcga tgtagccctg gtacggggcg
     2821 aagaatccgc ccaggaggcc ggtcaagaag gcgctcagga tgagtgagac cagcttgaac
     2881 cgggtgggat cgatgcccag ggcagtcgcg gcatcctggt cgtcccggag cgcccggaag
     2941 tagtagccgg ccttgctccg cagcgtgagc cagaccacaa ccatcgtcag caccgcgagg
     3001 gccaggacga tgtagtagaa ggggagccta tcccggaaga tcggcggaaa cagcaccccc
     3061 actggcccct tggtcaggga ccgccagttg atggcaatga tccgcaggat ctcggagatg
     3121 cccaggatcg tcagggcgaa gtatggccct cgcagccgaa aacagatgag cccgatgggg
     3181 atggacagga cggcggcaaa caggcccccc aggagcccgc ccaccaccag gggaatgccc
     3241 gggccgacgt agagcaggct cgtcacgtag gcgcccgtgc caaagaaggc cgcatgcccg
     3301 aaggagacct gcccggtaaa cccccccagc aagttccagc tttgccccaa acccacccag
     3361 agcaggaaca ggatcacaag gtgcaggtga tagggactcc gaacgaacag cggcagcagg
     3421 agaaagacca gcaggagaag gccgatcgcg atgcctcgcg gtccgaaggc acccaggacc
     3481 ccgacctgcg actgtctgtc cgtccgaccc atggaagacc tcaacccgtc gcccggttcg
     3541 cgtcagaatc gacttcggcc cacgagcccg gagggccgga gactgaggac aataatgaac
     3601 accaggtacc cgatgacgtc ccgatatgcc gtggacacgt acacggcccc cagcgactcg
     3661 gcaatgccca gagtgagccc accgaccagg gcgcccagaa cgcttcccat cccgcccagc
     3721 acggtcacga tgaatgcctt catggtgaag gagcccccca cggtcgggga gaggtagaaa
     3781 atcggcagaa gcagagaccc tgccgccccc gccagtccgg caccgatccc gaaggccagc
     3841 gtgtagaccc gatcaccgtc gatcccgtac agggatgccg catccttgtc ctgcgcggtc
     3901 gcccgcaagg accgaccgag atcggtcttc agcagcacgc tgtagaggag gagagagatc
     3961 accaaggcga tcgcgaaggc ggagagcagc gccaggctga ccgagatccc caataccgtg
     4021 agtgtggccg tggcgaacgg cgtgctgaat gtgcggtagt tggcagtgaa caggagccgg
     4081 accacctcgg taaggcccag gccgacaccc atggtcagga gcaactggtt ctcttccggc
     4141 gcgtttctgc ggaggagcgg gccgacgagg aattgctggg tcactgcccc caagaggaac
     4201 agcacgggga aagagatcag cacggagacg taaggattca cgccgaagag cgtgaagagc
     4261 caaaacgtga catacatccc caccatcagc atctcgccgt gggcgaggtt gatgactcgc
     4321 aacacgccga agataagact catcccggcg ccgatgagcg cgtagagccc acccagcagg
     4381 atgccgttca gcacggattg gagaaagacc tgactgccca ctcttcccgt ctcctttgtt
     4441 gggcagatgc cccttgcagg gagggcgtca cccacagggg ggagcggagg ctccccaacc
     4501 cgtgggtgag cgcctgaatc tcagtcggtt ctcctcccac tccgggagtg gatcaccgct
     4561 tcgaccacgg gggcgtgggg taggctgccg gggcggtcgc cacgtccttc ggccacaccg
     4621 tcacaaactt gcccttctgt acttggatga cgaaggtcgg cagcttggtc tgattctgga
     4681 atttcttata gctctcgaat cggatccgcc cgtacatcgt catcatgtcc gtcttgctga
     4741 gcgactcgac gatgtcgtcg ggcttgaggg acgcagcccg attcaggaca tccgccacca
     4801 cgaacacggc ggcataccct tgcgccccgt ggtaggatgg gggactcccg aatcgcgcta
     4861 tgtaatcctt gaggtagttc gccgccccag gataggggac cgacgcctcc cacatcgtcg
     4921 aggacaggac attctcggcc acatcgccgg cccccttgat gaaggggaga atgctgaacc
     4981 cggcggcggc ccccgtgaag gcctgagggt tgaggtccac ctccttcgcc tgacgcatca
     5041 gcaggaccgc gtcgaggaga taggacacca tgtaaatcac gtccgccttc ttctccttta
     5101 ctctgaggag gaggggtcgg taatccggtg cgccggcatc gtacggctcg tacatcaccg
     5161 cctcgtcgcg gttcttggtg gcccactctt tcatggccgc tgccgtgctg gtcccgaaca
     5221 gcgtgttctc gaacaggatg gcaatggact tgggcttggc taccttctcg aagaagtctt
     5281 tcaggccgtc gtcgaattcg ctcgccggct gcaccatgcg gaagatgtac ttccagccct
     5341 gctcggtgat ctgatcggcc gctgccgtgg agcacaggaa gggaatgccg tactgttgca
     5401 ccatctgcgc catcgggaac gtctccacgc tggagtactg cccggtcagc atcaccacct
     5461 tgtcctggtt gatcagcttc tcggtcacgg tccgggctgc ctgcacatcg ctgcgggagt
     5521 cctcgaagac caaatcgagc ttgtagcctc cccggacgcc gcccttggca ttcacctcgt
     5581 ccgcggcgag gacgaaggag ttcttgatga tctccccgaa cggggcatgt ctcccggtga
     5641 gagggagcgg aacgcccacc ttgatgctcc ccttgaactg agcagcctgc acggcaacac
     5701 ctggcagaag cgttacgaga acgaggcagg atgccaggag ccagagcgcg ggacggaagg
     5761 ttctgccggg gcatcgtttc cgccgaatca tcatcagacc ctccttttga gtatctggtt
     5821 gggaggacgc acaatctgga gcgccgttcc gaggggccgg gtgccggatg cgacagccga
     5881 gagattcgga attgcctcct ccggctttcg aatggttggc cctgcaattc cagaccttca
     5941 cctccctccc cgttgtatga gccggctaca cctgtcctgc cgttgggcgc cagactcaag
     6001 gcttcggagg gtcttcccca tcgcccccct cctcacccga gtgtcccagt tgctgagaca
     6061 ggcggtgtcc catcgtcttc acgaattccg taagctgagc cacccggtcc aggctcacgc
     6121 gccagaccgg acccgaaatt ccgatggcgg ccacgacatg gccggtgaag ttccgcaccg
     6181 gggccgcgag gcagcgaatg ccctgggcga actcttcatc atcgaaggca tacccctgct
     6241 ttcgcacccg cttcagttct tgttccagga tcggagccgc cgtgatcgtt tttgccgtaa
     6301 acggacggaa atcgctggcc tcgaggaacg tcttcaactc ggcttccggg aggtacgcca
     6361 ggagcgtttt tccgatggcc gtgcaatggg cagggcgggg gtatccaact ctgtcagaga
     6421 cccggacggg gctgctcccg tcgatcttgt cgattacaat aacttcgccc cgctcgagga
     6481 tcgccatgtg cgacgcctca ccagtttgcc gagccatttc caccaggaaa ggcttggcaa
     6541 tcttggagat ctgggcctcg gtgtaggctg acgcggcgag atgaaagacc ttcgagccta
     6601 ggaggtattc cctagtggtc tcatcctgga caagataccc tagcgtggag agggtgcgca
     6661 ggaggtggaa ggtcgtgctg gtatggaggc cgatttctcg actgacctcc gccattgtga
     6721 tgcctttgcc tttcttggcg atgatctcga ggatacccag cccccgttgc agcgactgga
     6781 tgatcggacc tccgattgcc tgattcctgt ctttcttcaa gctgtgttca ttcatgaagt
     6841 tatcctctcc tacttagttt cagtttgctc ccgtttcctg gcaggaaaag gtagaggatt
     6901 ctcgtaatgt gaaagtcttt ttgaatatat gaaaatatgc gtcgtgttaa ttacctaagt
     6961 atgaatagga ttttcattat gtgatttcaa taccaaattt atgtatggtt tgatgcatga
     7021 gtatgggatg caggcagcat tcagattgcc ccgaaagtat tcttagccgc ggacccgata
     7081 ccgaccgctc cccttgccac aaacccattg gcaggctata tagggccatg gacctctggc
     7141 gcgagttcca cggacccaac gccggttacg tgctggagct gtacgatcgt tatcgccaga
     7201 accccgcctc cgtagacgaa gccacccgcg ccttttttgc acgctggaca ccgccggcca
     7261 cgggaacccc cggatcgccg gcccccgcct gtgagaccat cgtcggcgca gtgaatcttg
     7321 cccagtccat ccgattctac ggccacctgg cggcgcggct cgaccccttg gggagtccgc
     7381 cgcgaggtga cccgtccctt ctgcccgaga cgtacggcat cacggaggcg gacctgcagc
     7441 ggctccccgc ccatctcatc ggaggaccgg tcgccgagcg tgccgccaac gcccatgagg
     7501 ccatccaggc cctgcgtgcg gtctattcct ccaccgccgg ctatggctat ggccacattc
     7561 aggcaccgga ggagcgggac tggctgcgcc acgctgccga gtcggggtgc ttccgctcgc
     7621 cccaggaccc catcaaccat cagaacctgc tgcagcggct cacccaggtg gaggccttcg
     7681 agcacttcct gcatcgcatc tttcctggca agacccgctt ctccctcgag ggcctggaca
     7741 tgctggtgcc catcctggac gagatcatcg gggaggcggc cgagaccgaa acccgtagca
     7801 tcctgatcgg catggcccac cgaggtcgcc tcaacgtcct ggcccatgtg ctcaaccgac
     7861 cctacgccca gatcctggcc gagttcaagg atcccacccg cggacggaac ttcaccatcc
     7921 gcgaagacct gagctggacc ggcgacgtgc gctatcacct cggggcgcac cgggctatcg
     7981 aggatggtat gccggtggac gtggtggtct ccatgccgcc aaatcccagt cacctcgaat
     8041 acgtgaatcc cgtggtggag ggcatggccc gagcggccgg cacccgtgcc gacgggccgg
     8101 gcccgcccct cttcgacccg cacatctccc tgccgatcct cattcacggt gatgcggcct
     8161 tccccggcca gggggtggtg gccgagaccc tgaatctctc ccgattgcct ggctaccaca
     8221 ccgggggtac gatccacatc atcgccaaca atcaactggg ctacaccacc ccgccggagg
     8281 acgagcgaag cacgctgtac gccagcgacc tggccaaggg gttcgagatc ccgatcgtcc
     8341 acgtcaacgc cgacgatccc gaggcgtgca tcgaggccgc cagactcgcc ttcgcctacc
     8401 gcgcccgttt ccacaaag
//
LOCUS       DBNGZC010000117         3640 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2455208, whole genome shotgun sequence.
ACCESSION   DBNGZC010000117 DBNGZC010000000
VERSION     DBNGZC010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2455208"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="AC16G0_02220"
     CDS             complement(<1..206)
                     /locus_tag="AC16G0_02220"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5098465.1"
                     /translation="MTLLDLLSHSAGRVPERPAFVFRERRMSYADLQRGALNLADRLL
                     TLGVNPGERVVLMLPYVPEFGLGY"
     gene            complement(300..1355)
                     /locus_tag="AC16G0_02225"
     CDS             complement(300..1355)
                     /locus_tag="AC16G0_02225"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HIR5098466.1"
                     /translation="MFQEIERVLGPAAKELLEHQCKGIPRELLHMPGPDFVDRVWGVS
                     DRTPQVLRSLQTLFRSGRLAGTGYLSILPVDQGIEHSAGASFAPAPIYFDPEKIVELA
                     LQGGCNAVATTFGVLGIVARKYAHKIPFIVKLNHNEFLSYPNRYDQILFGTVEEAWNL
                     GAVAVGATIYFGSDESTRQIQEVSVAFKRAHEMGMATILWCYLRNAAFKTAEKDYHVS
                     ADLTGQANHLGATIEADIVKQKLPENNGGYTALKFGKTHKKVYSDLTTDHPIDLARYQ
                     VANCYMGRIGLINSGGASAGESDLADAVKTAVINKRAGGMGLISGRKAFQRPMRDGVG
                     LLNAIQDVYLSPEITVA"
     gene            complement(1637..2623)
                     /locus_tag="AC16G0_02230"
     CDS             complement(1637..2623)
                     /locus_tag="AC16G0_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR5098467.1"
                     /translation="MSTVRIPRAARLLVVDDMEAIRLALEDSLRLQGYEVVSASSGEE
                     ALELMRSQCFDLLLTDQGMPGLTGIELAEATARIHPTMPVVLLTGHNDVELARASLQR
                     GASDFVTKPFSIRDLPILIERNLTRRRLEVKRLKEREGQVLFEAVKALASAVDAKDPY
                     TARHSMRVTRLSLILADAVGLSSEDKYVLELAAWMHDVGKIGVPDRILCKPACLTEEE
                     AGVMRSHPGKGGEIVGQIEEMTRVSEVIRHHHERPDGDGYPDGLRGEAIPLASRIILI
                     ADAFEAMTSNRSYRTGQGRDRAFDELRRYGGSQFDPDLVEVFITKVESLPPD"
     gene            complement(2620..>3640)
                     /locus_tag="AC16G0_02235"
     CDS             complement(2620..>3640)
                     /locus_tag="AC16G0_02235"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR5098468.1"
                     /translation="RTFWTLSRTLSVTLNMDELLRLTLHLIGRSLHVDAYSLMLLEEG
                     GKRLRVRAAFGLPEGARQDLSLSLGEGISGLVAQTGQAMLVPDVSAEPRFVEGALFGQ
                     MRGSFICVSLRIRGGEILGVLNAHRPDPQEFTGVDLDQFQAVANQVAAALENAQLYQR
                     TKELSSRDDLTGLFNRRAFFENLEKEVQRARRYRRAFSVLMLDLDHFKNYNDTHGHLK
                     GDEVLKELARLLLSNTRRADVAARYGGEEFVILLPEIGKQGGAVVAEKIRAAVEQSPF
                     YGRSQQPGGRVTVTLGLAAFPVDSEDGLELVDVADRAMYVGKQQGGNRVSVAPDRSAT
                     PSPVS"
BASE COUNT          675 a         1193 c         1113 g          659 t
ORIGIN      
        1 aaatacccca gaccgaactc gggaacatag gggagcatca agactacccg ttcgcccgga
       61 ttcaccccga gggtcagcag gcggtctgcc agattcaacg cgccccgctg gagatcggcg
      121 taggacattc gccgctcgcg gaaaacgaac gccggccgtt cgggaacacg gccggcgctg
      181 tgagacagca gatcaaggag ggtcatgggc cccgtgcggc gggcaccggc gggggaatcg
      241 gctggggcaa tgattcccac accggaactc gcaggctgag gacgtatccg cggtctgtct
      301 taggccaccg tgatctccgg ggacaagtac acgtcctgga tcgcgttcag caagccaacc
      361 ccatcccgca tgggacgctg gaaggccttc cggcccgaaa tcaagcccat gcccccggct
      421 cgcttgttga tgaccgcggt cttgacggcg tcggccaggt cgctttctcc cgcagacgca
      481 ccgccggagt tgatgagccc gatacgtccc atgtaacagt tggcgacctg gtaccgggcg
      541 aggtcgatcg ggtgatcggt cgtcaggtcc gaatagacct tcttgtgggt cttcccaaat
      601 ttcagcgccg tatagccgcc gttgttctcg ggcagctttt gtttgacgat atcggcctcg
      661 atggtggccc ccagatgatt cgcctgcccc gtaagatcgg cggacacgtg gtaatccttc
      721 tcggcggtct tgaaggcagc gttccgcaga tagcaccaga ggatcgtggc cattcccatc
      781 tcgtgggccc gcttgaaggc cacactcact tcctgaatct gacgggtgga ctcgtccgag
      841 ccgaagtaga tcgtggcgcc cacggccacg gcgcccaggt tccaggcttc ctccaccgtt
      901 ccgaagagaa tctggtcata cctgttcgga tagctcagaa actcgttgtg attgagcttc
      961 acgatgaagg ggatcttgtg ggcatatttt cgcgccacaa tccccaagac gccgaaggtc
     1021 gtggccaccg cgttgcaccc cccctggagg gccaactcca cgatcttctc ggggtcgaag
     1081 tagatcgggg ccggggcgaa ggaggcacca gctgaatgct cgatcccttg gtctacaggc
     1141 aggatggaga ggtatccggt tcccgctagc cgtccggaac ggaaaagcgt ctggaggctc
     1201 cgcaaaacct ggggtgtccg atccgagacg ccccataccc tatccacaaa atccggtccc
     1261 ggcatgtgca gcaactcccg ggggatacct ttgcactggt gctccaaaag ttccttggcg
     1321 gccggtccta acactcgttc aatttcctga aacatcgccg acctcctcgc cacccagggc
     1381 gcctcccatt gcgttcccgc cccacatgct ttgccagctc ggccaaaacc gaccggatcc
     1441 tggccggtca cggcgagcgt tcagaggcag ctggcgaaaa gtataacaga ccatccggtc
     1501 ggtcccaagc ggattcccgt ggattcggcc ggcggcacag cgggcgaacc actcccccgg
     1561 gttgtgccgg ccctattcgg aagagggagg tttccagagg gaacaagtat caggagtgca
     1621 cagggggcga gtggtctcaa tctggtggca gggattccac cttggtgatg aacacctcca
     1681 ccagatccgg atcgaactgg ctgcccccat acctgcgtag ctcgtcgaag gcgcggtccc
     1741 gtccctggcc cgtccggtag ctgcgattcg aggtcatggc ctcgaaggca tccgcgatga
     1801 gaatgatccg ggaggccaaa gggatggcct cgccccgcag accgtcggga tagccatcgc
     1861 catccggacg ctcgtgatga tgccgaataa cctccgagac ccgggtcatt tcctcgatct
     1921 gcccgacgat ctccccgcct ttccccgggt ggcttcgcat cacgccggcc tcttcctccg
     1981 tcaggcaggc tggcttgcac aggatacgat ctggcacgcc gatcttgccc acatcgtgca
     2041 tccaggcggc cagttccagg acgtacttgt cctccgagga caagccgact gcgtctgcca
     2101 agatgaggga cagccgggtg acccgcatgg agtggcgggc tgtgtagggg tccttcgcat
     2161 ctacggccga tgccagggcc ttcacggcct cgaacagcac ctggccctcg cgctccttca
     2221 accgcttcac ctcgagccgg cgccgggtca gatttcgttc gatgaggatc ggcagatctc
     2281 ggatgctgaa gggtttggtc acaaagtcgc ttgccccccg ctggagcgag gcccgggcta
     2341 gctccacgtc attgtgtccc gtgagcagga ccacgggcat ggtgggatgg atgcgagcgg
     2401 tggcctcggc caattcgatg ccggtgagac cgggcatccc ctgatccgtc agcagcaggt
     2461 cgaagcattg cgaccgcata agttccagcg cctcctctcc cgacgaggcc gacaccacct
     2521 cgtacccctg gagccggagg gagtcttcca gggccagacg gatggcctcc atatcatcca
     2581 ccaccaggag gcgcgctgcg cgggggattc gtaccgtgct catgacactg gggacggggt
     2641 tgcgctgcgg tcgggtgcga cactcacgcg gttacccccc tgttgctttc cgacgtacat
     2701 ggcccgatcg gccacgtcca ccaattccaa gccatcctcc gaatccaccg gaaacgccgc
     2761 cagccccagg gtcaccgtga cccggccgcc tggctgttgg ctgcggccgt agaaggggga
     2821 ttgctccacc gcggcccgga tcttctccgc caccacggcc ccgccctgct tgccgatttc
     2881 cggcagcaag atcacgaatt cctcaccgcc atagcgggcc gccacgtcgg cgcggcgggt
     2941 gttgctgagg aggagacgcg ccaactcctt cagcacctcg tcgcccttga ggtggccgtg
     3001 ggtgtcgttg tagtttttga agtgatccag atccagcatg aggacggaga aggcccgacg
     3061 gtagcggcgg gcccgctgga cctctttttc cagattctcg aaaaaggccc gccgattgaa
     3121 aaggcccgtg aggtcgtcgc gagaactcag ctccttggtc cgctgataaa gttgcgcgtt
     3181 ctccagggct gccgcgacct gattggccac cgcttggaac tgatccagat cgaccccggt
     3241 gaattcctgc ggatccggcc gatgcgcatt gagcaccccc agaatctcgc cccccctgat
     3301 ccgcagggag acgcagatga aggacccgcg catttgacca aacagggccc cctcgacaaa
     3361 acgcggctcc gcgctgacgt cgggaaccag catggcctgg ccggtctggg cgacgaggcc
     3421 agagatcccc tcccccaggg agaggctgag atcctgcctt gcgccctcgg gcagtccgaa
     3481 ggccgctcga accctcagac gtttcccacc ctcctccagg agcatcagcg agtaggcatc
     3541 cacgtgtagc gaccgcccga tgagatggag ggtcaggcgg agcagctcgt ccatgttcag
     3601 ggtgaccgac agcgtccggc tgagggtcca gaaagtccgg
//
LOCUS       DBNGZC010000118         2289 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1965913, whole genome shotgun sequence.
ACCESSION   DBNGZC010000118 DBNGZC010000000
VERSION     DBNGZC010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1965913"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="AC16G0_02240"
     CDS             <1..935
                     /locus_tag="AC16G0_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR5098469.1"
                     /translation="LLDAEGPTGGKLVINGKPVRSIGFMHCVGSMQQKGIHPPTPDGK
                     THAYCSRVCCTACLHAETELKARDPDLHVFDFHEDIRTYGRWHEDYYSRASEEGVAFV
                     RFNPLRPPRVERDPRGQAPLVVRTVDRLTFGEEIEVPLDMLVLATGLVAGEMTRLVDL
                     LRCSVGPDRFLLEVHPKLRPVELAVFGLFLAGSVQGPMDTVESSAGAAAAAAKASVLI
                     SQGSIELDPFIARVTEELCSGCRICLSVCPYDAITRDEAKRVAAINESVCTGCGTCVA
                     TCPCNAITQFGFTDQEVKSEVLALLGKQLAAVAA"
     gene            949..1455
                     /locus_tag="AC16G0_02245"
     CDS             949..1455
                     /locus_tag="AC16G0_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HIR5098470.1"
                     /translation="MAEVSAAHQQPQAAPASWEPRIIGFLCYWCSYTGADSAGTARMK
                     YPPNVDIIKVMCSGRIDPELITTAFANGADGVLVLGCHIGDCHYISGNHKTMVRMPLM
                     RRVLEDLGIEPERFRHEWVSAAEGEKFSKLVAEITEQVRELGPLKWPQLVHERGVGHG
                     MDLKAWGD"
     gene            1459..>2289
                     /locus_tag="AC16G0_02250"
     CDS             1459..>2289
                     /locus_tag="AC16G0_02250"
                     /inference="COORDINATES: protein motif:HMM:NF013242.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIR5098471.1"
                     /translation="MGANGKLKIGMYWASSCGGCDISLLEIGPRLLDLIKVADVAFWP
                     CAADFKYQNVAAYPDGYIDACFYNGGVRNSEQEEVARLLRKKSRTLIGYGACAAEGGI
                     PALANLKSGAGIYDIVYHTNPSIDNRDRVEPQPSVETPVGTLEIPTFYPGVLRLKDIV
                     PVDYEIPGCPPQADRVWESIQAVVTGAVPPRNAAVKVGCTGKTVCDECSLEKKLTKIK
                     QFKRHHEFRPEPGWCLLEQGLLCMGPATRSGCGALCLKAGMRCEGCYGPPVNAEDQGM
                     S"
BASE COUNT          395 a          760 c          762 g          372 t
ORIGIN      
        1 gactcctcga cgcggaaggg cccaccggtg gcaagctggt catcaacggc aagccggtcc
       61 ggagcatcgg cttcatgcac tgcgtgggtt ccatgcagca gaaggggatc caccccccca
      121 cccccgatgg aaagacccac gcctactgca gccgcgtctg ctgcacggcc tgcctgcacg
      181 cggaaaccga gttgaaggcc cgcgaccccg atctgcacgt cttcgacttt cacgaggata
      241 tccggaccta cggccggtgg cacgaggact actacagccg cgccagcgaa gagggggtgg
      301 ccttcgttcg cttcaacccg ctccgccccc cccgggtgga gcgggatccc cgtggccagg
      361 ccccgctcgt ggtccggacg gtcgaccggc tcaccttcgg tgaggagatc gaggttccct
      421 tggacatgct ggtcctcgcc acgggcctgg tggccgggga aatgaccagg ctggtggatc
      481 tgctccgctg ctcggtgggg ccggaccggt tcctgctgga ggtgcacccg aagctccggc
      541 cggtggagct ggcagtcttc ggcctcttcc tggcggggtc ggtccagggg cccatggata
      601 ccgtggagtc ctcggccggc gctgccgccg ctgccgccaa ggcgtcggtc ctgatcagcc
      661 aggggagcat cgaactcgac cccttcatcg ccagggtcac cgaagagctt tgcagcggct
      721 gccggatctg cctgagcgtc tgcccctacg acgccatcac ccgcgacgag gcgaagcgcg
      781 ttgcggccat caacgagtcg gtctgcactg gctgcggcac ctgcgtggcc acctgcccct
      841 gcaacgccat cacccagttc ggttttactg accaggaggt gaagtccgag gtcctggccc
      901 tgcttggcaa gcaactcgcg gccgtcgccg cctaagggga gatcagccat ggccgaagta
      961 tccgccgctc accaacagcc gcaggcggca cccgcctcgt gggagccgcg catcatcggc
     1021 ttcctctgct actggtgctc ctacaccggg gcggatagcg ccggcacggc gcgaatgaaa
     1081 taccccccca atgtggacat catcaaggtg atgtgctcgg ggcggatcga cccagagctg
     1141 atcaccaccg ccttcgccaa cggcgccgac ggggtgctgg tcctggggtg tcacatcggc
     1201 gactgccact acatctcggg caaccacaag accatggtcc gcatgccgct catgcgccgg
     1261 gtcctcgagg acctcggcat cgagccggag cgcttccggc acgaatgggt cagcgccgcg
     1321 gagggggaga agttcagcaa gctggtcgcc gagatcacgg aacaggtccg ggagctcggt
     1381 ccgttgaagt ggccgcagtt ggtgcacgag cgcggcgtgg gacacggcat ggacctcaag
     1441 gcgtggggag actagccaat gggcgccaac gggaagctca agatcggcat gtactgggcc
     1501 agtagctgtg gcgggtgcga catctcgctg ctggagatcg gtccccgcct gctcgacctg
     1561 atcaaggttg ccgacgtggc cttctggccc tgcgcggctg acttcaagta ccagaatgtt
     1621 gcggcctacc cggatggcta catcgatgcc tgtttctaca acggcggcgt ccggaacagt
     1681 gaacaggagg aggtcgcccg gctgcttcgg aagaagagcc ggaccctcat cggctacggg
     1741 gcctgtgcgg cggagggcgg catcccggcc ctggccaact tgaagagcgg ggcggggatc
     1801 tatgacatcg tctaccatac caacccctcc atcgacaacc gggacagggt cgagccccag
     1861 cccagcgtcg agacgcccgt gggaacactc gagatcccca cgttctatcc tggcgtgctc
     1921 cgcctcaagg atatcgtccc cgtggactat gagattccgg ggtgcccccc tcaggcagac
     1981 cgcgtctggg aatccatcca ggcggtcgtc acaggggcgg tccctcctcg caatgcggcc
     2041 gtgaaggtcg gttgcacggg gaaaaccgtg tgcgatgagt gctctctgga gaagaaactg
     2101 acgaagatca agcagttcaa acggcaccac gagttccggc ccgagcccgg ctggtgcctg
     2161 ctggagcagg gcctgctgtg catgggaccc gccacccgca gcggctgcgg cgcgctctgc
     2221 ctcaaggcgg ggatgcgttg cgagggctgc tatggcccgc cggtcaacgc cgaggatcag
     2281 ggcatgtcc
//
LOCUS       DBNGZC010000119         2688 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_792383, whole genome shotgun sequence.
ACCESSION   DBNGZC010000119 DBNGZC010000000
VERSION     DBNGZC010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_792383"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="AC16G0_02255"
     CDS             complement(<1..1169)
                     /locus_tag="AC16G0_02255"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098472.1"
                     /translation="MLRRIGITMVGLLMVLGFAFGTQAQEVVKIGIVAPLSAPGGVET
                     GQALVDGAKIAAEELNASGGLLGKKVDLVIGDTSGQPEKGTAVMERLATNDKVIAVGG
                     EAHSSVALAEIEVAHRYGIPLVISEAWSDSVTAKGYPEVFRVTVSNSLIYSKAARWIK
                     DSGFKHVAVIGENSDWGLGVIQVFKDSLSSAGVKVTSMSAERTVTDFTPQLMQLKGMT
                     PPVDFLVDGFTGTGELLMIKQAYQLGFAPSKATAVLGAGMDTLYPGFWDTVGDAGVHV
                     LSNPAGLPGLPKTKTSEKFVKAFKAKLNREPDAVAMEGYDTLMVIAEAVKAAKSTSGK
                     ALVDALENKVNWEGTRGTIKFSKEKSPAWAYHMWMDVPVFIIQFTKVKQDFSQAA"
     gene            1970..2341
                     /locus_tag="AC16G0_02260"
     CDS             1970..2341
                     /locus_tag="AC16G0_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope biogenesis protein OmpA"
                     /protein_id="HIR5098473.1"
                     /translation="MKLLLILGVILFLAGCAATNPVLSPNAHLQQVGKDQAQRDISEC
                     QDLADRSVQPTPAERVAQETAIQPRRGESVGVAGGGINVAPPPVLTPAPAAPTRGSSA
                     WKDFVSRCLRDRGYELAGWEK"
BASE COUNT          471 a          893 c          838 g          486 t
ORIGIN      
        1 atggcggcct gggagaaatc ctgcttcacc ttggtgaact ggatgatgaa caccgggacg
       61 tccatccaca tgtggtaggc ccaggcgggg gacttctcct tgctgaactt gatggtgccg
      121 cgggtcccct cccagttcac cttgttctcc agcgcatcca ccagcgcctt cccgctggtg
      181 gacttggcgg ccttgacggc ctcggcgatg accatgagcg tatcgtagcc ttccatggcc
      241 accgcgtccg gttcccggtt cagcttggcc ttgaaggcct tgacaaactt ctctgaggtc
      301 ttggtcttgg ggaggccggg aagaccggca gggttggaga ggacgtggac ccccgcgtca
      361 ccgaccgtgt cccagaagcc cgggtagagc gtgtccatgc ccgcccccag gacggcggtg
      421 gccttcgagg gcgcaaagcc gagctggtag gcttgcttga tcatcagcag ctcgccggtg
      481 cctgtgaagc cgtccacgag gaaatccaca ggcggcgtca tgcccttgag ctgcatgagc
      541 tgcggggtga agtcggtgac ggtgcgctcg gccgacatgg aggtcacttt caccccggcc
      601 gatgacaggc tgtccttgaa cacctgaatg acgccgagcc cccagtccga gttctcgccg
      661 atgacggcca cgtgtttgaa cccggaatcc ttgatccacc tggccgcctt ggagtaaatg
      721 agggagttgc tgacggtgac ccgaaacacc tccggatagc ccttggcggt gacggaatcc
      781 gaccaggcct cggagatgac cagcgggatg ccgtaccgat gggcgacttc gatctcggcc
      841 agtgccaccg agctgtgcgc ttcgcccccg acggcaatga ccttatcgtt ggtggcgagg
      901 cgctccatca cggccgtgcc cttctcgggc tgtccgctgg tgtccccgat gactaggtca
      961 accttcttcc ccaggagtcc gcccgaggcg ttcagctcct cggcagcgat cttcgcccca
     1021 tccacgagcg cctggcccgt ctcgactccc cccggagcgg atagcggggc cacgatcccg
     1081 atcttgacca cctcctgggc ttgcgtgcca aatgcaaatc ccaagaccat caggagaccg
     1141 accatggtaa tgccaatcct gcgaagcata ctacccctcc ttccattccc accaggttgc
     1201 cagtcgacca ccaaagcaag cgccgggcgc caagcgccaa acaccagagg tcagagaaca
     1261 gcggtcagca aagaccgtgc ccgtgctggt ctctgacttc tgacctctgt cctctgccct
     1321 gggctcaatg cgccatgcgc ccagagggct gatagactga cgtctacgca cctacccttc
     1381 tggtagtgaa gatccttgtc cctgcgtatc gcgaaacgca agcgggtgat gctacctcgg
     1441 aggtgggggt gaggaccctt cgtgttcggg ccggacggcc agcgcgccct caccagtcca
     1501 gtgcggctac ggcccctccc gccagcgccg cgaagcatgc agcagaagag ggagcagtgt
     1561 caagccgaat ctatgccgca gaccggtgag cgcgcacacg cacggcctgc gtcgcctgca
     1621 gtgccgacac cctcgcgatc cagatccacc tccggtcgga gatcgcttcg agcggcgggg
     1681 tccagtccct gagaaattcg gaatggaaat ccgtggccaa gtacgacgac gggtgtgaaa
     1741 gctgctcccc ccaacaagtg aagagagttg cgcgtgaatc tggagcgtag tatctttcct
     1801 gtacgatgct gtagggagga tcggcattca gtttcgcggc cgcggaacgg gggacgctcg
     1861 cggcctctag caccacattt cccaggcggg ggtgagcctt ggccaggagc ccgctcggtg
     1921 gtggttgtct ttgcgatcag actgggccca ttggaggatt ggaattcaca tgaagctctt
     1981 gctcatcctg ggtgttatct tgttcctcgc cggctgcgcc gccaccaatc ccgtcctctc
     2041 ccccaacgcg cacctccagc aggtggggaa ggaccaggcc caacgcgaca tctcggagtg
     2101 ccaggacctt gccgatagat cggtccagcc cacccccgcc gagcgagtag cccaggagac
     2161 ggccatccag cctcggagag gagagagcgt cggcgtcgcg ggtgggggca tcaatgtggc
     2221 tccgccccct gtcctcaccc ctgcgcccgc tgctcccact cgaggttcat cggcctggaa
     2281 ggatttcgtc agccgctgcc ttcgggaccg cggttacgag ctggctggct gggagaagtg
     2341 atgctggggt gagctgccga tgacggcgtc ccgccgcgga tggctgaggc tgaagcccag
     2401 gtaacggact gccggaatct cacggcccac cgtactcggc agcgtctcag accctgggcg
     2461 ggagttccag ttgccggatt gccgaccgcc atgtgccggt ccacggcagg aggacctgcg
     2521 cgctgtccgc ggccgtcggc tcaatgctgc ctcggcttcg ggaccgtcac cccgcggaga
     2581 cgcagcctgg tttcccgcca ggccgcctgc aggagtcccg gcccctgaca cacccagacc
     2641 ccggccaggg tcaagccccc tccgaacaac tgggtgaacg tgagtctg
//
LOCUS       DBNGZC010000120         1807 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_792451, whole genome shotgun sequence.
ACCESSION   DBNGZC010000120 DBNGZC010000000
VERSION     DBNGZC010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_792451"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..596
                     /locus_tag="AC16G0_02265"
     CDS             <1..596
                     /locus_tag="AC16G0_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098474.1"
                     /translation="TRISWRRRLQTTTSGVCTRHRLLCIQQGAVACLAFLLVSSGAAL
                     AWGVFTEQILIPLPSTPTAVAAGPVAEAGGLWLRVKFAAWDRSHAYARAKHPILTNER
                     PARRLEVRMSLQNPTDQPQAVSPHEFQLRARSGAVWPAVSVLPVTLVRPKQAVTSILA
                     FNVPAQAAGLQLVWTKAGHEVRIPLGDAASSQDRDGS"
     gene            685..1635
                     /locus_tag="AC16G0_02270"
     CDS             685..1635
                     /locus_tag="AC16G0_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016491336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR5098475.1"
                     /translation="MRRTIVSAIAFILAVMTLTSAGLPPALAAPPGSPWGASYFPNVP
                     LITQDGQTVHFYDDLLKGKAVVINLIYTSCKDECPLETAKLVQVQKLLGDRVGKEVFF
                     YSISIDPKRDTPTVLKAYAAKFHVGPGWLFLTGNKQDIDLISKKLGLSSLTDAANPDG
                     HQASLMLGDEATGQWMRHSAVDNPQYLAVAIGSLLAGGRAHPQGRSYAEAAPIPMPAQ
                     GRHLFAGQYLFQTRCAACHTLGKGDGVGPDLRGVTTRRDRAWLTRFIATPDRVLAERD
                     PLALALFAQYQEVRMPNLRLGDEDVAALLQYLEAQAVAGK"
BASE COUNT          312 a          639 c          556 g          300 t
ORIGIN      
        1 ccacgcgaat atcgtggcgg aggcggctgc agaccacgac ctcgggggtt tgcacgaggc
       61 accgattgct gtgcatacag caaggcgcag tggcctgtct ggcgttcctc ctcgtctcca
      121 gcggggctgc cctggcgtgg ggcgtgttca cggaacagat attgatccct ctcccatcaa
      181 cccctacggc tgttgccgcc gggccggtgg ctgaggccgg cggtctctgg ctccgggtga
      241 agtttgcagc ctgggatcgc tcccacgctt atgcgcgagc caaacatccg atactcacca
      301 atgagcgacc ggcgcgtcgc ctggaggtcc ggatgagcct ccagaacccc accgatcagc
      361 ctcaggcggt cagcccccat gagttccagc tccgggcccg gagtggcgcc gtctggccgg
      421 ccgtgagtgt tctcccggtg accctggtga ggcccaagca ggccgtgacc agtatcctgg
      481 cgttcaacgt gcccgctcag gctgcaggac tccagctcgt gtggaccaaa gccggacacg
      541 aagtccggat cccgctgggg gacgccgcct ccagccaaga tcgtgacggg tcctagtgag
      601 agaccacaag agagccgcag ggtcgccccc gtgggctccc tccgaccgct gctcgcggcc
      661 gatcctgcat ggagacccca catcatgcga cgcacgatcg taagcgctat cgccttcata
      721 ctcgctgtca tgaccctaac ctccgcgggt cttccccccg ccctggccgc gccccccggc
      781 tccccgtggg gggcgagcta ctttcccaac gtccccctga tcacccagga cgggcagacg
      841 gtgcacttct acgacgacct gctcaagggc aaggccgtgg tgatcaacct catctacacc
      901 tcctgcaagg acgagtgccc cctggagacc gccaagctgg tccaggtgca gaaactgctg
      961 ggcgaccggg tgggaaagga ggtcttcttc tactccatca gcatcgatcc caagcgggat
     1021 actcccaccg tgctcaaggc ctatgctgcg aagttccacg tcggacccgg ctggctgttc
     1081 ctaaccggca acaagcaaga tatcgacctc atcagcaaga agctgggtct ctcctccctg
     1141 accgacgcgg ccaacccgga cggccaccag gccagcctca tgctggggga tgaggccacc
     1201 ggtcagtgga tgcgccactc cgcggtggac aacccgcagt acctggccgt ggccatcggc
     1261 agtctactgg ccggggggcg ggcccacccg cagggcagaa gctacgccga ggcggccccc
     1321 attccaatgc ccgcgcaggg gcggcatctc ttcgcaggcc aatacctctt ccagacccgc
     1381 tgcgcggcct gccacaccct cggcaaaggc gacggggtcg ggccggacct ccgaggggtg
     1441 accactcggc gggaccgggc ctggctcacc cgcttcatcg ccaccccgga ccgcgtgctg
     1501 gcggagcgcg accccctcgc cctggccctc tttgcccagt accaggaggt gcggatgccc
     1561 aacctgcgcc tgggggacga agatgtggcc gccctgcttc agtacctgga ggcacaggcc
     1621 gtcgccggga aatgatcgga aggggcgcag actgttgcga ctcaccgcct cgatcgccct
     1681 gaggaagttg aaggtggtga gccacacgaa cagtccttgt cccgaaatcc ctgtagtttt
     1741 cttgcgtgat ctagtgtcct gtcccagagg ttagataaac aagtccggcc gttccggccc
     1801 gcccctc
//
LOCUS       DBNGZC010000121         2770 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_107812, whole genome shotgun sequence.
ACCESSION   DBNGZC010000121 DBNGZC010000000
VERSION     DBNGZC010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_107812"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="AC16G0_02275"
     CDS             <1..912
                     /locus_tag="AC16G0_02275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5098476.1"
                     /translation="TNRQYTQNIHVFAPASWFILARNVSPGQRFDKGTELYRLADLSQ
                     VWILADVFENEGQHFKRGSTVKVSLTNQQRTFQARVSDVLPLFDATSRTLKVRLEADN
                     PGFLLRPDMFVDLEVPVMRPPAVTVPVDAVVDSGIKKVVYVDKGEGIFEPRKVETGWR
                     HGDRVEVVKGLMPGERIVISGTFVLDSESRMKAAAAGIYDETSEDPVCGMEVDQAKAK
                     AAGLTSQFRGQTYYFCSDDDKVKFDKEPTRYAGGAVEKKEVLRPKHLRGVEWKGAPVL
                     APETWGGTRREMGRTSGSPSHQGQPHD"
     gene            905..>2770
                     /locus_tag="AC16G0_02280"
     CDS             905..>2770
                     /locus_tag="AC16G0_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5098477.1"
                     /translation="MINRIIEFSVKNKFIIFLLIAAAGIIGWWSMKHLPLDAIPDLSD
                     TQVIIYSRWDRSPDIVEDQVTYPIVNAMLGAPRVKAVRGFSDFGYSYVYVIFEDGTDI
                     YWARSRTLEYLSTALARLPQGVRTEMGPDATGLGWVFQYALVDTSGKHSLADLRSYQD
                     WYLRSYLKAVPGVAEVAPIGGFSRQYQVNVDPNRLQAYGIPINRVVDAVRGGNNDVGG
                     RLIEFGGTEYMVRGKGYVRSIRDLENIVLTASESGTPIRIKDIGQVVVGPDLRRGVSD
                     LDGTGEAVSGIVILRQGENALDVITRVKAKLREIAPGLPPGVKVIPIYDRSELITRAI
                     GNLRSTLIEIMLTVAFVILLFLWHFPSAIIPMLTIPIAVLVAFIPFRLMGVSANIMSL
                     GGIAIAMGALVDAAIVVVEQTHKKLELWGRTDRQSDYRSVILTAVQEVGGPSFFALLV
                     IAVSFLPVLTLEAQEGRLFKPLAYTKTLSMLVAAVLAITLDPALRLLFTRLKDFDFRP
                     RWLCRAANGLLVGSIHPEEQHPLSRRLIRLYEPVATWALRWKWLVIGGACALMLITIP
                     IYQRLGAEFMPSLDEGALFYMPSTMPGISIGEAQKLLQVTDRLLKQFPEVDRVLGK"
BASE COUNT          493 a          884 c          894 g          499 t
ORIGIN      
        1 acgaaccgcc agtacactca gaatatccat gtcttcgctc cggccagctg gttcattctc
       61 gcccggaacg tctcccctgg ccagcgtttc gataaaggta cagagttata ccggctggca
      121 gatctcagcc aggtctggat cctggcggac gttttcgaaa acgaggggca gcacttcaaa
      181 cggggctcga cggtgaaggt atcgctcacc aatcaacagc gcacttttca ggcaagggta
      241 agcgatgtcc ttccactgtt cgacgcgacg agccggacat tgaaggtccg cctggaggcc
      301 gataatcccg ggttccttct tcgcccggat atgttcgtgg atctcgaggt gccggtgatg
      361 cggcctccgg ccgtcaccgt cccggtggac gcggtcgtgg actcggggat caagaaggtg
      421 gtgtacgttg acaagggaga gggaatcttt gagccgcgga aggtggagac ggggtggcgg
      481 cacggcgatc gggtggaggt cgtcaaggga ctcatgcccg gtgagaggat cgtcatttcg
      541 ggtacctttg tgctcgactc cgagagccgg atgaaggcgg cggcagcggg aatctatgac
      601 gagaccagcg aggacccggt ctgtggaatg gaagtggatc aggccaaggc caaggcggcc
      661 ggactgacga gccagttccg ggggcagacc tactatttct gctcggacga cgacaaggtg
      721 aaattcgaca aggagccgac ccgctacgcc ggcggggcag tcgagaagaa agaagtgctc
      781 aggcccaagc atctccgggg cgtcgaatgg aagggagctc cggtgctcgc gccagagaca
      841 tggggaggta cacgacggga gatgggccgg acctcaggct cgccgagcca ccagggccag
      901 ccccatgatt aaccgcatca tcgagttctc ggtcaagaac aagttcatca tcttcctgct
      961 catcgccgca gccgggatca taggctggtg gtcgatgaag cacctgccct tggacgccat
     1021 ccccgatctg agcgacaccc aggtcatcat ctattcacgc tgggaccgca gccccgacat
     1081 cgtcgaggac caggtcacct acccgatcgt caacgccatg ctgggggcgc cgcgcgtgaa
     1141 ggccgtccgc ggcttctccg acttcggcta ttcctacgtc tacgtcatct tcgaggacgg
     1201 gaccgacatc tactgggccc gctcccggac gctggagtac ctctccacgg cgctggcacg
     1261 ccttcctcag ggtgtgagaa cggagatggg gccggacgcc accggtctcg gctgggtctt
     1321 ccagtacgcc ctcgtggaca cgtccggcaa gcacagcctt gcggacctcc ggtcctatca
     1381 ggactggtac ctccgcagct acctcaaggc cgtgccgggc gtggccgagg tcgcgcccat
     1441 cggggggttc agccgacagt accaggtgaa cgtggatccc aaccgcctcc aggcctacgg
     1501 tatcccgatc aaccgtgtgg tggatgccgt gcggggcggc aacaacgacg tgggcggccg
     1561 gctgatcgag ttcggcggga ccgagtacat ggtccggggt aaggggtatg tccgctccat
     1621 ccgcgacctg gagaacatcg tcctcacggc cagcgagagc gggacgccca tccggatcaa
     1681 ggacatcggc caggtggtcg tgggcccgga cctgcggcga ggggtctcgg acctcgacgg
     1741 gaccggggag gccgtctccg ggatcgtcat cctgcggcag ggggagaacg ccctggatgt
     1801 gatcacccgg gtgaaggcca agctccggga gatcgccccc ggactccctc ccggcgtcaa
     1861 ggtgatcccg atctatgacc gctcggagct gatcacccgg gccatcggta acctcaggtc
     1921 caccctgatc gagatcatgc tcacggtggc cttcgtgatc ctgctcttcc tctggcattt
     1981 ccccagcgcc atcatcccga tgctcaccat ccccatcgcg gtcctcgtcg ctttcatccc
     2041 cttccgcctc atgggggtga gcgctaacat catgtccctc gggggcatcg ccatcgccat
     2101 gggggccctg gtggacgcag ccatcgtggt ggtggagcag acccacaaga agctcgagtt
     2161 atgggggcgg accgaccgcc agtcggatta ccggagcgtg atcctcaccg ccgtgcagga
     2221 ggtggggggg cccagcttct ttgccctcct ggtcatcgcg gtctccttcc tgccggtgct
     2281 gaccctggag gcccaagaag ggcgcctgtt caagcccctg gcctacacca agaccctctc
     2341 catgctggtg gcggctgtcc tggccatcac cctcgacccg gccctgcgcc tgctcttcac
     2401 gcgcctgaag gacttcgatt tccggccccg ctggctatgc cgagcggcga atgggctcct
     2461 ggtggggagc attcatcctg aggagcagca cccgcttagc cgccgcctca tccggctgta
     2521 cgagcccgtg gccacctggg cgctccgctg gaaatggctg gtgatcggcg gggcctgcgc
     2581 cctgatgcta atcacgatcc ccatctatca gcggctgggc gcggagttca tgccctctct
     2641 ggacgagggg gctctcttct acatgccgtc gacgatgcca gggatctcca tcggcgaggc
     2701 ccagaagctt ctccaggtca cggaccggct cctcaagcag ttccccgagg tggaccgggt
     2761 cctggggaaa
//
LOCUS       DBNGZC010000122         1570 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_10227, whole genome shotgun sequence.
ACCESSION   DBNGZC010000122 DBNGZC010000000
VERSION     DBNGZC010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_10227"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..1327)
                     /locus_tag="AC16G0_02285"
     CDS             complement(join(195..889,889..1327))
                     /locus_tag="AC16G0_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611506.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HIR5098478.1"
                     /translation="MEKYRVTLTDEERTALEQLISKGKAAARRLTHARILLLADDSRG
                     GGCPDEEIVATLDASPRTVARVRRRLVTEGFQAALDHRPQPARPDKIKIRGDAEQKLV
                     ELACTDPPQGRCRWTLQLLADEMVVLGLVAAISIETVRQALKKTNIRPWIVETWCLPP
                     EADAEFVWRMEDVIRTYLLPYDPKYPVVCFDEACKQLFGEVRPPRRARRGKPARVDYE
                     YERKGVCHQLVMCEPLRGWRHIAVTERRTRRDYARCVKDLVDRYYPRAEAIRLVQDNL
                     NTHDGASLYEAFPPEEARRLLDRIEFHYTPKHGSWLNMAETEINIMDQQCLDRRLDSQ
                     GRIAEEVAAWEKERNAREARIHWTFTLAAARRKLRKVYPSIEA"
BASE COUNT          280 a          500 c          482 g          308 t
ORIGIN      
        1 aagatgaccc ggtggaggtg gatggtttaa gtccagtgtc cgctaacatg aggcgatgac
       61 gacttcaact ggtcgcaagc cagaaacccc ttgaattcaa gtgaaaaacc aacatagacg
      121 atagaacaga taagcagtac tgttgtccag atgttgcctg ttttgcctcc atgggaatcc
      181 aggctagtgg ggcttcaagc ttcgattgac gggtaaactt tccgcaactt ccggcgtgca
      241 gcggccaggg tgaaggtcca gtggatgcgt gcctcccggg cattccgctc cttctcccag
      301 gccgcgacct cttcggcgat ccgaccctgg ctgtcgagcc gacgatccag gcactgctga
      361 tccatgatgt tgatctccgt ctccgccatg ttcagccagc tgccgtgctt gggcgtgtag
      421 tggaactcga tccggtccaa gagccgacgc gcttcctcgg gcgggaacgc ttcgtacagg
      481 ctcgcaccgt cgtgcgtgtt caggttgtct tgcaccaagc ggatcgcctc ggcgcgcggg
      541 taatatcggt ccaccaagtc cttcacacat cgggcatagt cccgccgggt gcgccgctcg
      601 gtcaccgcga tgtgacgcca gccccggagc ggctcgcaca tcaccagttg gtggcacacc
      661 cccttccgct cgtactcgta gtccacccgg gcgggcttgc cccggcgcgc ccggcggggt
      721 ggtcgtacct caccgaagag ctgtttgcac gcctcgtcga agcagacgac cggatatttc
      781 gggtcgtacg gcaacaagta ggtccggatc acgtcctcca tgcgccagac gaactcagcg
      841 tcggcctccg gtgggaggca ccaggtctcg acgatccagg gccggatgtc gtttttttca
      901 gcgcctgacg caccgtctcg atgctgatcg cagcgaccag gccgagcacg accatctcgt
      961 cggccagcag ctgcaaggtc catcggcacc ggccctgggg cggatcggtg caggcgagtt
     1021 cgaccagctt ctgttcggcg tccccccgga tcttgatctt gtccggcctg gcgggctggg
     1081 gccggtgatc gagggccgcc tggaacccct cggtgacgag ccgcctccgg actcgggcga
     1141 ctgtccgcgg gctggcgtcc agcgtagcga cgatctcctc atcggggcat ccccctcccc
     1201 gggagtcgtc cgccaggagg aggatgcggg cgtgagtcag cctgcgggcc gcggccttcc
     1261 ccttggagat cagctgttcc aacgcggtcc gttcctcgtc ggtcagggtg acgcgatact
     1321 tctccatggg aggattcctg tacctgaggg ccgtgccatt gcccgctgcc tcaacggaca
     1381 ggataccaaa tttacccgtc aatagaagct tgaagcccca ctaagcgctt aacgtttggt
     1441 cggacaacga accaccatcg tagtaaagag aggaggtgtt tggagggcag ttatcatgag
     1501 accctattcg atcgatctcc gccagcgcgt cgccgccacc ctcgatgaaa acgagggctc
     1561 gcagcggcag
//
LOCUS       DBNGZC010000123         5852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1183698, whole genome shotgun sequence.
ACCESSION   DBNGZC010000123 DBNGZC010000000
VERSION     DBNGZC010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5852
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1183698"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="AC16G0_02290"
     CDS             complement(<1..590)
                     /locus_tag="AC16G0_02290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014923.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
                     /protein_id="HIR5098479.1"
                     /translation="MTAFFATASAGTEDLLADELRELGLPGVRPVRGGVRFTGKTVDA
                     MRVCLWSRIAMRVLLPLAEFPAATADALYAGARDIPWQEHLDETRTFAVEAVGRTEAL
                     GHTHYTALKVKDAVADALRERRGTRPSVDVRDPDVHIVAHLAGGRASLALDLAGEPRF
                     KRGWRLAQTQATLKETLAAAILRACGYEGTRPLIDP"
     gene            complement(922..1674)
                     /locus_tag="AC16G0_02295"
     CDS             complement(922..1674)
                     /locus_tag="AC16G0_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0120010 - intermembrane phospholipid
                     transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HIR5098480.1"
                     /translation="MGARVRVVSAGCAALLVCGCSTLTQTGPNSLPPLRTYEAVVTDG
                     GPHLLEVDDPIEGFNRGTYRFNYYVDEYLYRPVVRAYEIVLPDYVKDRISDALDNIGE
                     FGNLTNNLLQIKFKDAGITLSRFLLNSTAGFAGLWDPATAIGLTRKPADFGQTLGHYR
                     VGSGPYLVLPALGPSNVRDTIGLAADSAEFSLVGPVAWVNEPAVSGAYGGISGIDKRH
                     RIPFRYHQTGSPFEYDLLRMLYTIKREFDVAH"
     gene            complement(1689..2975)
                     /locus_tag="AC16G0_02300"
     CDS             complement(1689..2975)
                     /locus_tag="AC16G0_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5098481.1"
                     /translation="MNRRFAVILVVACFSYAKCVDAASPSYSYPYDNPYEATVLGLPP
                     SLEVQLPAAVPTREFRLKVFPEREIPEVFWYEDGLVCSLVYQDHPAPLVFVIGGTAAH
                     YDSPTVVKLQKLLYQAGFHAIGITSPGHMDFVVNATTDLPGDSLGDARDLYRVMRLAY
                     EQVRERITVSSFALTGYSLGAFNAAFLAQLDAEEKQFNFTRVLLINPPVNLYTSVSLL
                     DQLLVDNIPGGMDRLDDWFRSVLGHVLAVSRQMGRTELSGEFLYNIYDSLPHDQKNLQ
                     ALIGLVFRLSAAHMIFTADVMNGGGYIVPTQARLTNSTSLTRYAMVAYRTRFVDYFND
                     VLLPHLQKEEPGLTRQALLERLSLRRLEPYLRGADTVGLVHNADDIILAPGELQYLEG
                     VFGTRAHIFPSGGHLGNVFHPEVARTIVGFLTGKDN"
     gene            complement(3518..>5852)
                     /locus_tag="AC16G0_02305"
     CDS             complement(3518..>5852)
                     /locus_tag="AC16G0_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5098482.1"
                     /translation="SPYYQQSALYPVIDHLQRVLQWQRYATPEAKFDALERVLRTYRL
                     PLEEVIPLFATLLSVPLPERYPPLTLPPQQQRQKTHEALIAWLLEETERQPVLAVWED
                     LHWADPSTLEHLSLVLDQAPTARMLILLTCRPEFRPPWAAHVPVTQVTLTRLARPPVE
                     AMILHLTGGKALPAEVVQQIVAKTDGVPLFVEELVKMVLESGLVREEDDRYVLAGPLP
                     PLAIPSTLHDSLMARLDRLSAARELAQLGAVLGREFAYDVLQGVSPLDEMTLQRGLAQ
                     LVEAEMLYQRGLPPRSRYVFKHALIQDAAYQSLLKSTRQQYHQRIAQVLEARFLEAVE
                     RHPELVAQHYTGAGLHEQAVTYWQRAGARALQHSANAEAIAHCRAGLELLPTLPQTPA
                     RLQQELALQITLGPALIATRGHAAPEVEHTYARARELCQQVGETPQLFRALRGLWLFY
                     NVRAAHQTARELGEQCLRWAQHVQDPALLLEAHRPLGVTLFCLGEVAAARAHLEQGIA
                     LYDPQQHRSHAFLYSIDPGMACCSYVAWALWLLGYPEQALQRSQEAILLAREHLLSQA
                     QALSWAAWLHQLRREPHAARERAEKAVALCSEQRFPYFLAWGTILQGWALATQGEGEA
                     GIAQMRQGLVAHRATGAEEHRPFFLALLAEAYERAGQAEEGLSVLAEALAAVDRTGER
                     VYEAELYRLKGELLQKQAVVVGAVEECFRHAVSVARQQSAKSLELRAVISLSSLLKAQ
                     GKTAEARQMLTEIHAWFTEGFDTADLREAKALLEMHA"
BASE COUNT         1084 a         1836 c         1928 g         1004 t
ORIGIN      
        1 atcgggtcga tcagcggccg cgtgccctcg tagccgcagg cacgcaggat ggccgcggcc
       61 agcgtctctt tgagagtcgc ctgggtttgc gccaggcgcc agccgcgctt gaagcgcggc
      121 tcgccggcga ggtcgagggc caggctcgcc cggccgccgg cgaggtgcgc cacgatgtga
      181 acgtcggggt cgcgaacgtc cacgctgggt cgcgtcccgc gacgctcgcg cagggcatcc
      241 gccaccgcgt ccttcacctt gagggcggtg tagtgggtgt gccccagggc ctcggtgcga
      301 cccacggcct cgacggcgaa ggtgcgtgtc tcgtcgaggt gctcctgcca ggggatgtcg
      361 cgagcgccgg cgtacagggc gtcggcggtc gcggcaggga actcggcaag gggcagcagc
      421 acccgcatgg cgatgcgtga ccacaggcag acgcgcatgg cgtcaacggt cttgcccgtg
      481 aagcgcacgc cgccgcgcac ggggcgcacg ccgggaagac ccagctcgcg cagttcgtcg
      541 gccagcagat cctcggtgcc ggccgaggcg gtggcaaaga aagcagtcac gcgccctcca
      601 gcttcgcgaa ttccggccgg ccgtccggtg gggacttcac ggacgttcct ctttcccagg
      661 cttccggacg cccttctcgt gtccaccccc gccggcgaca ctgaaccagc gcgactctgc
      721 cgcaaccgta tcgagggtgt caagaggaaa cccagcccga gtacctacag tctcagacag
      781 gaattgtaag acggggaggc cggaagccga tcctcaccga gtagggagga gtttgagtcc
      841 tcgttggctc cgcgccaaag gcgaatctgg gcgctggaaa gtgagcgtcg cggccggagc
      901 gatcagaccg gtgggtaaac cttaatgggc cacgtcgaat tctcgcttta tggtatagag
      961 catgcgcagc aggtcatatt caaacggcga accggtttgg tggtaccgga aggggatgcg
     1021 gtgccgcttg tcgatgccgc tgataccccc gtaggctcca gaaacggcag gttcattgac
     1081 ccaggcaacg ggaccgacga ggctgaactc agccgaatct gccgcgagcc cgatagtatc
     1141 ccggacgttg gacggcccca gcgccggcag cacgagatat ggaccgctgc ccaccctgta
     1201 atgtcccagc gtctggccga agtccgcagg tttccgcgtg agtcctatgg cggtggcagg
     1261 atcccacaga cccgcgaagc ctgccgtgga attaaggagg aagcggctca aggtgatgcc
     1321 agcatccttg aacttgatct gaagcaggtt gttggtcagg tttccgaatt cgccgatgtt
     1381 gtcgagagca tccgagatcc gatccttcac atagtccggc aagacgattt cgtaggctcg
     1441 cacgacgggg cggtacaggt attcgtcaac gtaatagttg aaccggtagg taccgcggtt
     1501 gaaaccctcg atggggtcat cgacctcgag caggtgtggg ccgccatcgg tcacgacggc
     1561 ctcgtaggtt cgcagaggag gcagactatt cggccccgtt tgcgtgagag tgctgcagcc
     1621 gcagaccagg agcgcagcgc aacctgcgct gacgacacgt acccgtgctc ccatgaaagc
     1681 cgccatggtc aattgtcctt tccggtgaga aacccgacga tggtccgggc gacctcgggg
     1741 tggaacacgt tccccaggtg gccgccgctg gggaagatgt gcgccctcgt gccgaagacg
     1801 ccctccagat actgaagctc gccaggcgcg aggatgatgt cgtcggcgtt gtgcacgagt
     1861 ccgaccgtgt cggcgccccg gaggtaaggc tcaagcctcc gcaggctcaa gcgctccagg
     1921 agcgcctgcc gggtgagacc tggttcctcc ttctggaggt gcggcagcag gacatcgttg
     1981 aagtagtcga caaaccgggt gcggtaggcg accatggcgt aacgggtgag cgacgtggag
     2041 ttggtgagcc gggcctgcgt cgggacgatg tatccgccgc cgttcatgac gtccgcggtg
     2101 aagatcatgt gggcggcgct taacctgaac acgaggccga tcaaggcctg gaggttcttc
     2161 tggtcgtgcg ggaggctgtc gtaaatgttg taaagaaact cgccgctcaa ctcggtacgg
     2221 cccatctgcc ggctgacggc aagtacgtgc ccgaggacgc ttcgaaacca gtcgtccagc
     2281 ctatccattc cccccggaat gttgtccacc agcagttgat ccagcaacga caccgaggtg
     2341 tagaggttga cgggcggatt gatgagcagc actctggtga aattgaattg tttttcctcg
     2401 gcgtccagtt gggcgaggaa ggcggcattg aaggcaccga ggctataccc cgtcagagcg
     2461 aagctcgaga cggtgatccg ctcgcgcacc tgttcatagg ccagtcgcat gacccggtag
     2521 aggtccctgg catccccaag ggaatccccg gggaggtcag tcgtggcgtt taccacgaag
     2581 tccatgtggc cgggcgaggt gatgccaatt gcatgaaagc ctgcctggta gaggagtttc
     2641 tgaagcttca ccacggtcgg cgagtcgtag tgcgccgcgg tgccgccgat gacgaagacc
     2701 agcggggcgg gatggtcctg gtacaccagg gagcagacca ggccgtcctc gtaccagaac
     2761 acttcgggga tctcgcgttc tgggaagacc ttcagcctga actcgcgggt ggggacggct
     2821 gccgggagtt ggacctccag tgagggcggc aaccccagga cggtggcctc ataggggtta
     2881 tcgtagggat aggaatacga cggagacgca gcatcgacgc acttggcata ggagaaacag
     2941 gccacaacaa ggatgacggc gaatcgtcgg ttcatcctgt cctccgcaga acatcagtta
     3001 gcgagtcggc tcgaggtgcc gcgtccggga ggggctcggt acaccgaacc tcggctcgaa
     3061 catcataaag catggaacat gctccggagc acaactcctc tcgggcgcag accggcagag
     3121 gcagtaacaa tgaaccactg agggtgcccc agcaaaggcc tccgcccgtt cagtatagaa
     3181 tggtagtgga ctgactggaa gtttgccagt catgcccggt ccgaagacaa gggatggtcc
     3241 gatcgagttg cgtcgaaacg tcaggaagaa gagggcgaac gtgctgacca ggagccgtct
     3301 cagaagcgaa taggctgggc gcgttcacgt gccggccgtg ccacgatgac gcgcaatcct
     3361 atcagatcct ccgctccgcg ctgttcgtca ttatcgatgc ccgacagcgg aacgcgattt
     3421 ggcaggccac cccgcgagaa gctgcatgag gatcgcgagt gcccgatcca cgaatctggc
     3481 agccgccgca tagggggatt gaccaccctc cgtcgcctca tgcgtgcatc tccagcagtg
     3541 ctttggcctc ccgtaggtcc gccgtgtcaa agccctcggt gaaccacgca tgaatctccg
     3601 tcagcatctg tcgagcttct gctgtcttgc cttgcgcctt caagagagaa ctcaggctta
     3661 tcaccgcccg cagctccaga gatttcgcac tttgttggcg cgccacactc acggcgtgac
     3721 gaaaacattc ttccactgca cccacaacta ccgcttgttt ctgtaataac tcccccttca
     3781 gccgatatag ctctgcctcg tagacacgct cgccagttct gtccactgcc gccagtgcct
     3841 cggccagcac gctcaatcct tcttcagcct ggcccgctct ctcatacgcc tcggccagca
     3901 gggcaagaaa gaacggtcgg tgctcctctg cccccgtggc ccggtgagcg accagcccct
     3961 ggcgcatctg ggcaattccc gcctcgccct ccccctgcgt ggccagcgcc cagccctgca
     4021 ggatcgttcc ccaggccaaa aaatacggaa acctctgctc gctgcacagg gctaccgcct
     4081 tctccgcgcg ctctcttgct gcgtgtggct cccggcggag ctgatggagc caggccgccc
     4141 agctcagggc ctgcgcctgg ctcaagaggt gctcccgggc caggagaatc gcctcctggc
     4201 tcctctgcag agcctgctcc ggatagccca gcaaccacaa ggcccacgcc acatagctac
     4261 agcaagccat cccagggtca atgctgtaga ggaacgcatg ggagcggtgc tgctggggat
     4321 cgtagagagc aatcccctgc tccaggtggg cgcgggcagc cgcgacctct ccgagacaga
     4381 acaacgtgac tcccaggggc cggtgggcct ccagaaggag tgcggggtct tgcacatgct
     4441 gggcccagcg gaggcactgc tcccccagct cccgtgccgt ctggtgcgcg gctcgcacat
     4501 tgtagaacag ccacaatccc cgcagtgccc ggaagagctg tggggtttcc cccacctgct
     4561 gacacagctc ccgcgcccgg gcgtaagtat gttccacttc tggggccgca tggcccctgg
     4621 tggcgatcaa ggcggggccg agcgtgatct gcaaggccag ctcctgttgc aggcgggcgg
     4681 gcgtctgcgg gagcgtgggg aggagctcca ggcccgcgcg gcagtgggcg atggcctccg
     4741 cattggccga gtgctggagg gcgcgcgcac cggcccgctg ccagtaggtc acggcctgct
     4801 catgaaggcc tgccccggtg tagtgctgtg ccacgagttc tgggtgcctt tcgacggcct
     4861 cgaggaaccg cgcctccaat acctgggcaa tccgctggtg gtactgttgc cgggtgctct
     4921 tgaggagcga ctgataggcg gcgtcctgaa tcagggcatg cttgaagacg taccggctcc
     4981 gggggggcag cccgcgctgg tagagcatct cggcctcgac caactgcgcc agcccgcgct
     5041 gtaacgtcat ctcatccaag ggcgagaccc cctggagcac gtcgtaggca aactcccgcc
     5101 ccaggacggc gccgagctgc gccagctctc tggccgctga gagccggtcg agccgggcca
     5161 tcagtgaatc gtgcagggtt gagggaatcg ctaagggggg cagcgggccg gccagcacat
     5221 agcgatcgtc ctcctcccgc acgagaccgg attcgaggac catcttgacg agctcttcga
     5281 caaacagggg caccccgtcg gtcttggcca cgatctgctg caccacctcc gcaggcaggg
     5341 ccttgccgcc cgtcaggtgg agaatcatcg cttcgaccgg gggccgcgct aagcgggtga
     5401 gggtgacctg cgtcaccggc acatgagcgg cccacggcgg gcgaaactcc gggcgacagg
     5461 tcaacaggat cagcatccgc gccgtcggcg cctgatcgag caccagactc aggtgctcga
     5521 gcgtcgaggg atcggcccag tgcaggtctt cccagacggc cagaacgggc tgccgctccg
     5581 tctcctcgag cagccaggcg atcagggctt cgtgggtctt ctgccgctgc tgttggggcg
     5641 gcagggtgag cgggggatag cgttctggca acggcacgga gagcaaggtg gcgaagagcg
     5701 ggatgacctc ctccaggggc aggcgatacg tgcgcagcac ccgctccagc gcgtcgaact
     5761 tcgcctcggg ggtggcgtac cgctgccatt gcaggactcg ctgcaggtgg tcgatcaccg
     5821 ggtacaatgc gctctgctgg tagtagggag aa
//
LOCUS       DBNGZC010000124         5660 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1672883, whole genome shotgun sequence.
ACCESSION   DBNGZC010000124 DBNGZC010000000
VERSION     DBNGZC010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5660)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5660)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5660
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1672883"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="AC16G0_02310"
     CDS             complement(<1..706)
                     /locus_tag="AC16G0_02310"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIR5098483.1"
                     /translation="MRLHPLDPMLAAVMRRRAAAGRRPRPLAEFDPTRVRSILAVSST
                     AIGDTLFATPAWRALRQRYPTARIVGHVRDKVLPLFSDNPHLDAVIPYAGGYRRFLGT
                     VAALRGERFDAVVILHGMGPQAIPMAVLSGAPCVIRIPNSGEYGYLLSNALPSPDNLP
                     YPGEHAIQCRLRMAGMLDAKSDDVRMVLPVTQEDRRAAQALLARAGVDPETPTLGLVP
                     GAATTFKQWPPERFAAA"
     gene            complement(720..2648)
                     /gene="asnB"
                     /locus_tag="AC16G0_02315"
     CDS             complement(720..2648)
                     /gene="asnB"
                     /locus_tag="AC16G0_02315"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR5098484.1"
                     /translation="MCGIAGLVRFDGDKIGDAPERMVAALRHRGPDDSGVAYFDAARG
                     MALGTQRVDAERANAALGFARLSIIDLTPAGHQPMANEDGTIWIVFNGEVYNFAEVRP
                     WLLERGHRFRSRSDTEVLLHAYEEEGVGCLERFRGMYGLAILDLPRRRLFLIRDRLGL
                     KPVKYVANSRVLAFASELKALCALPEVVRRLDPAAVEAYLTYRYVPAPHTGLEGVCKL
                     PPASILELDLAGGTHRITRYWRPPRGTPVHRPEAEVVAEAQARFDEAVALRLIADVPV
                     GVFLSGGIDSSAVVASAVRAQPDLQTYSVGFDDPRFDELAYARTVAARFGTRHHELVV
                     RPALGGDMEAVVRAFDEPFADPSAVPSFYLAKATAAHVRVALNGDGGDELLGGYKRYR
                     VHRRTWRWQRWLPERRQWLARAAAMLPPDVDKKRWRGRLGRLLLEASLPYERAYQLRF
                     SGLDPRSREDIRGPWLRERMSGQDPLNRLAQVFAASPESDPLVRLLDVDLVTNLPDDI
                     LAKADLAGMAHSLEARSPFLDHRFVEFVTQLPRHLRTDRKLLLRRMLADVLPPEILGR
                     KKMGFNPPIEGWIRGELSGLVRDALLSPGAITGPVLERRTVQAMVDHHLAGRGNLGER
                     IWLLLVLELWVRIFGVTL"
     gene            complement(2648..3829)
                     /locus_tag="AC16G0_02320"
     CDS             complement(2648..3829)
                     /locus_tag="AC16G0_02320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5098485.1"
                     /translation="MRVAYVDPQAYPSATPEALQVFHTCVGLASHAEAVWLVGGRGRP
                     TDVAAYYGVVQPANLRLLRFPRFRREQGWVRPAWTAPFHFLALARLRRLVRVQEIQAV
                     LTRDLKLAQFFLRAHRRRPLPPVVFESHEIFADTLREAEVRSGRGLSTKLERLAAREA
                     EVYREAGGLIVLTHALAALLRQRFATRGHIQVAPDGVDLAGPLPVAANAANGPVTYLG
                     SFHPWKGVEILVRALASVPEARLRLVGGAPEPRARIEGLAKAMGIADRVDCVGPVPPP
                     ARWQYLAEAGMCVLPLIRSAYGTSFTSPLKLFEYMAAARPILASNLPALREVLRDEEN
                     GLLVPPDDPEALAAAIRRLRADPPLANRLASQAARDVRAYSWDARGKSILEFLGSLGT
                     D"
     gene            complement(3867..4976)
                     /locus_tag="AC16G0_02325"
     CDS             complement(3867..4976)
                     /locus_tag="AC16G0_02325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5098486.1"
                     /translation="MGLRLCHLESSAGWGGQEIRVLTEAAGLRERGHEVWIVCKPESV
                     LALRTREAGLPLILDRMPFAHDPRTIARLIGHFRRLRPQVVVTHSSVDSWCGGVSAWT
                     LRIPVVRMRHLSVSIGDHPATRFAYRSLCHTVITTGGAIREHLIRDVGIPSDKVVSIP
                     TGIDTARFDPRKADGAKVREALGIPRDAPLAGMVAVLRDWKGHRVFLQAMVEVRKRVP
                     AVRALIVGDGPQQRNIERDRREFGLEGVVVLTGHREDIPDVLASLDVVVSASTGAEGV
                     PQALLQALAMERPVVATAVGAVPEIIRDGETGRLVAPRDPLQLAGVLEAVLRDPAAIR
                     PLALAGAALVRAQWDIGHMLEAVERVYTRVAAPPW"
     gene            complement(5033..>5660)
                     /locus_tag="AC16G0_02330"
     CDS             complement(5033..>5660)
                     /locus_tag="AC16G0_02330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5098487.1"
                     /translation="EICREYTQRVYVDEWRGFGAHKNLCLSRAAHPWVLSLDADERVT
                     PALQTAIQEVLAAEGSLDGYFIPRRNYFLGRWVRSSGWYPDHVLRLFRKARGKFKEVA
                     VHEAVEVEGKVGYLEEPLEHLTYRSISDFLSRMDRYSGLAAEEMLRQGRKAGGLDLVL
                     RPPVTFLRMFLLRGGFREGWHGLVLAGLYASYTFAKYARLWEMRRAGK"
BASE COUNT          969 a         2079 c         1807 g          805 t
ORIGIN      
        1 ctgccgccgc aaaacgctcc ggaggccatt gcttgaaggt ggtggccgct cccgggacca
       61 gccccagcgt gggcgtctcc gggtccactc cggcgcgcgc cagcagggcc tgcgccgccc
      121 gccggtcctc ctgggtgacc ggcaagacca tccgcacatc gtccgacttc gcgtccagca
      181 tgccggccat gcgcaagcgg cactggatgg cgtgctcgcc cggatacggc aggttatcgg
      241 gagaggggag agcattcgag agcaggtatc cgtattcacc gctgttggga atccggatca
      301 cgcagggcgc ccctgacagg accgccatgg ggatggcctg cggccccatg ccatgcagga
      361 tgacaaccgc atcgaagcgc tcccctcgca gcgctgccac cgtgccaagg aatcgtcgat
      421 atcccccggc atagggaatc acggcatcca ggtgcgggtt gtccgaaaac aggggaagga
      481 ccttgtcccg cacgtgcccg acgatcctgg ccgtggggta ccgctggcgc aacgcccgcc
      541 aggcgggagt ggcaaacagc gtgtccccga tcgccgtcga agagaccgcc aggatgcttc
      601 gcacccgggt cggatcgaac tccgccaacg ggcgaggacg acggccggcg gccgccctcc
      661 gccgcatgac cgctgccagc atgggatcca gcggatggag gcgcatcagc cagccgacct
      721 cacagagtca ccccgaaaat ccggacccac aactcgagca ccagaagcag ccagatccgc
      781 tccccgaggt tgccccgtcc cgcgaggtga tgatccacca tggcctgaac cgttcggcgc
      841 tccaggaccg ggcccgtgat ggcacctgga ctcaggagtg cgtcccgcac cagacctgat
      901 agctcgccgc gaatccatcc ctcgatcggc ggattgaagc ccatcttctt tcggccgagg
      961 atctccggcg gcagcacatc cgccagcatc cgccgaagca gcagcttccg gtccgtccgg
     1021 aggtggcggg gcaattgcgt gacgaactcc acgaagcggt ggtccagaaa gggggatcgc
     1081 gcctccaggc tgtgggccat tccggccaga tccgccttcg ccaggatgtc gtcggggaga
     1141 ttggtcacga gatccacatc cagcagacgc accaagggat ccgactccgg agaggccgcg
     1201 aaaacctgcg ccagccgatt caacgggtcc tgaccgctca tgcgctcgcg gagccacggc
     1261 ccccggatgt cctcccgcga ccgtggatcc aggccgctga agcgcaactg gtaggcccgc
     1321 tcgtacggga gagacgcttc gagcagcaga cgccccaggc gcccgcgcca gcgtttcttg
     1381 tccacgtccg ggggcagcat cgccgcggct cgcgccagcc actggcgccg ctccggtagc
     1441 cagcgctgcc agcgccacgt gcggcgatgc acccgatacc gcttgtaccc gccgagcagc
     1501 tcatccccgc catccccgtt cagcgccacc cggacatgcg cggccgtggc cttggccagg
     1561 tagaagctgg ggacggccga cggatcggcg aacggctcgt cgaacgcccg aacgacggcc
     1621 tccatgtccc cgcccagagc cgggcggacc accagctcgt ggtggcgcgt cccgaaccgt
     1681 gccgcgaccg tgcgggcgta ggccagctcg tcgaagcgtg ggtcatcgaa gcccacggag
     1741 taggtctgaa gatccggctg ggcccggacg gccgacgcca ccacggcgct ggagtcgatc
     1801 cccccgctga gaaagacccc caccgggaca tcggcgatca gccggagggc caccgcctcg
     1861 tcgaaccgcg cctgtgcctc ggccaccacc tccgcctccg gccggtggac cggcgtcccg
     1921 cgcggcggcc tccaataccg ggtgatccgg tgggtgcccc cggccagatc cagctccagg
     1981 atcgaggctg ggggaagctt gcagaccccc tccagcccgg tgtggggggc cgggacgtac
     2041 cggtaggtga gatacgcctc caccgccgcc gggtccaatc gacggaccac ctcgggcaac
     2101 gcgcagagcg ccttcaactc cgaggcgaaa gccaggactc tcgaattggc gacgtacttt
     2161 accggcttca gccccaggcg atcgcggatc aggaagagcc gccgccgtgg gagatccagg
     2221 atcgccagcc cgtacatccc tcggaatcgc tccaggcatc cgacgccttc ttcctcgtac
     2281 gcgtgcagca ggacctcggt gtccgagcgg gagcgaaatc ggtgtccacg ctccaggagc
     2341 caggggcgta cctcggcgaa gttgtagacc tcgccgttga acacgatcca gatcgtgccg
     2401 tcctcgttgg ccatgggctg gtggcccgca ggggtgaggt cgatgatgct cagccgggcg
     2461 aaccccagcg ccgcattcgc cctctcggca tccacccgct gcgtgcccag cgccatgcct
     2521 cgagcggcgt cgaagtaggc gaccccggaa tcgtcggggc cccgatgccg gagcgccgcc
     2581 accatgcgtt cgggggcgtc cccgatcttg tctccatcga accggaccag gccggcgatc
     2641 ccgcacatca gtcagtcccc agactgccaa ggaactccag gatgctcttg ccccgagcgt
     2701 cccacgaata ggcgcggaca tccctggcgg cctgactcgc cagccggttg gccagcggtg
     2761 gatcggcccg gagtcgccgg atcgccgccg ccagcgcctc cgggtcgtcg gggggaacca
     2821 gcaatccgtt ctcctcgtcc cgcaagacct ctcgcagggc gggcagattc gaggccagga
     2881 tgggccgggc cgccgccatg tactcgaaga gcttcagcgg tgacgtgaag gaggtgccgt
     2941 aggccgaccg gatgaggggc aggacgcaca tcccggcctc cgccaggtac tgccaccgag
     3001 ctggaggcgg cacggggccg acgcagtcca ccctgtccgc gatgcccatg gccttcgcca
     3061 atccctcgat ccgcgcccgg ggctcgggtg cgccacccac cagcctgagc cgcgcctccg
     3121 gcacggaggc cagggcccgg accagaatct cgactccctt ccacggatgg aagctgccca
     3181 agtaggtcac gggtccattc gcggcgttcg cggccaccgg gagcggtccg gccaggtcca
     3241 ccccgtcggg cgccacctgg atatgtccac gggtcgcgaa gcgctggcgg agcagggccg
     3301 ccagtgcgtg ggtgagcacg atgaggcccc ccgcctcgcg atagacctcc gcctcgcggg
     3361 ccgccagtct ctccagcttg gtcgaaagac cccgccccga acggacctcc gcctcccgca
     3421 gtgtgtcggc gaagatctca tggctctcga agaccaccgg cggaagcggc cggcggcgat
     3481 gcgcccgcag gaagaactgc gccagcttca ggtcccgcgt caagacagcc tggatttcct
     3541 gaacccggac gagccgacgc agcctggcca atgccaggaa gtggaacggg gcggtccagg
     3601 cgggccgtac ccaaccctgc tcccgccgga agcgcgggaa tcgcaggaga cggagattgg
     3661 cgggttgcac cacgccgtag taggctgcca catccgtcgg gcgcccccgt cctcccacga
     3721 gccagacggc ctccgcatgg gacgccaggc cgacgcaggt gtggaagacc tggagcgcct
     3781 ctggggtcgc cgaggggtag gcctgggggt ccacatacgc cacgcgcatc gccgtcacgc
     3841 tgctccaggc tccaggctgg tcaggctcac catggcgggg ccgccaccct ggtatacacc
     3901 cgctccaccg cctccagcat gtgtccgata tcccactgcg cacggaccag cgctgctccc
     3961 gccagagcca gcggccggat cgctgcggga tcccgcaaga ccgcttccag gactcctgcc
     4021 agttgcaaag ggtcacgcgg ggccaccagt ctccccgtct ccccgtcgcg gatgatctcc
     4081 ggcaccgcgc ccacggccgt ggcgaccacc ggtcgctcca tggccaacgc ctgcaggaga
     4141 gcttgcggaa cgccctccgc ccctgttgag gcggacacca ccacatccag gctcgccagg
     4201 acatccggga tgtcctcccg gtgtccggtg aggacgacca cgccttccag cccgaattcc
     4261 cgtcgatccc gctcgatgtt cctctgttgc ggcccatcgc cgacgatgag ggctcgcacc
     4321 gcggggacgc gtttccgcac ctccaccatg gcctgcagaa aaacccgatg acccttccag
     4381 tcccggagga ccgccaccat gccggccagc ggtgcgtccc gcgggatccc cagggcctca
     4441 cgcaccttgg ctccgtcggc cttccgggga tcgaagcgcg ccgtgtcgat ccccgtcggg
     4501 atgctgacga ccttgtcgga agggatcccc acgtctcgga tcaggtgctc gcggatcgcc
     4561 ccgcccgtgg tgatgaccgt atggcacagc gatcggtacg cgaaccgggt cgccggatgg
     4621 tccccgatgg acacggacag atgccgcatc cggacgaccg ggattcggag cgtccacgcc
     4681 gacacccccc cgcaccagct atccacggag ctgtgggtca ccaccacctg tggccggagc
     4741 cggcgaaagt gcccgatcag cctggcgatc gtccggggat cgtgggcaaa gggcattcga
     4801 tccaggatca ggggaagccc ggcctcccgc gtcctgagag ccaagacgct ctccggcttg
     4861 cagacgatcc agacctcgtg tccccgttcc cgcagaccgg cggcctcggt caggacgcga
     4921 atctcctgcc cgccccaacc agcggaggat tcaaggtgac agagacgcaa ccccatgagt
     4981 cctggctctc aactcccgcg gccattcgcc gaccggaact cggcccccgc cgtcacttgc
     5041 cggcgcgcct catctcccag agtcttgcgt acttggcgaa ggtgtaggag gcgtagagac
     5101 ccgccagcac cagaccgtgc cacccttccc ggaagccgcc tcgaagcagg aacattcgca
     5161 ggaacgtgac cggcggcctg agaaccagat ccaggcctcc ggccttccga ccctgtcgaa
     5221 gcatctcctc cgccgccagg ccggaatacc ggtccatccg gctcagaaaa tcgctgatcg
     5281 agcggtaggt gagatgctcc aggggctcct ccagataccc gaccttccct tcgacctcca
     5341 cggcttcatg cacggcgact tctttgaact tccctctggc cttccgaaag aggcggagga
     5401 cgtggtcggg ataccagccg gaggatcgga cccagcggcc caggaagtaa ttccgccgtg
     5461 gaataaagta cccgtccagc gagccctccg ccgccagcac ctcttggata gcagtctgca
     5521 gggccggcgt cacccgctca tcggcgtcga ggctcagcac ccacgggtgt gcggcgcggc
     5581 tcaggcaaag gttcttgtgc gccccgaagc cccgccattc atccacgtag acccgttgcg
     5641 tgtactcccg gcagatctcc
//
LOCUS       DBNGZC010000125        14135 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2357768, whole genome shotgun sequence.
ACCESSION   DBNGZC010000125 DBNGZC010000000
VERSION     DBNGZC010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 14135)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14135)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14135
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2357768"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..507)
                     /locus_tag="AC16G0_02335"
     CDS             complement(<1..507)
                     /locus_tag="AC16G0_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5098488.1"
                     /translation="MNMTLLETEEDILVIDAGLMFPEEEMLGIDLVLPEVTYLLEQRE
                     KVRGIVLTHGHEDHTGGLPYLLPQLSVPIHGTTLALSLVQEKLREFGLDEAADLRRIR
                     PRDVLHLGGFTLEFIRVSHSIPDGVAVAVQTPAGLVIHSGDFKFDQTPVDGELTDFQR
                     FAALGEQGV"
     gene            complement(674..3055)
                     /gene="lon"
                     /locus_tag="AC16G0_02340"
     CDS             complement(674..3055)
                     /gene="lon"
                     /locus_tag="AC16G0_02340"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR5098489.1"
                     /translation="MPSQIPLLPVRDVVVYPFMILPLFVGRDKSIRAVDEALSRERLI
                     LLVAQRDADSEDPGPEDLHPVGTVAMIMRMLKMPDGRVKILVQGVSRAKILGFTRQDP
                     CFEARIAEVAEPETGPQGLEVEALIRSVKEQIQKGVNLGKGVSPDVLVIINNLEHPGR
                     LADLVASNLDLKVEQAQQILEIFDPVERLKRLSELLGKEIEVLEMQHKIQSQAREEMD
                     KTHREYYLREQLKAIQKELGETDERGQEIQELEQKIEKAKMPPAVAQEAKTQLGRLSR
                     MHPDAAEASVIRTHLDWLIDLPWAKQTQDKLSIKQAHKILEADHYDLEKVKERILEYL
                     AVRSLKKKLKKKMKGPILCFVGPPGVGKTSLGRSIARALGRKFIRISLGGVRDEAEIR
                     GHRRTYIGALPGKIIQGIKQAGTNNPVFMMDEVDKIGADFRGDPSAALLEVLDPEQNE
                     TFTDHYLGVAFDLRNVMFITTANLADPIIPALKDRMEVIEISGYTEEEKLHIAKRYIL
                     PRQLEEHGISKKLLDLTDDAILKTVQSYTREAGLRNLEREVATICRKVARDVAEGKKG
                     PTRVTGASLDRFLGAPKFLPEPEQEKDEIGVATGLAWTQTGGDILYIECTMMRGKGIL
                     SLTGHLGDVMKESAQAAMSYARSRGTTLGIKEETFAKHDVHIHVPAGAIPKDGPSAGI
                     TMATALISAMTKTPVRRDVAMTGEITLRGKVLPIGGLKEKALAARRMGIKTVIVPAKN
                     DKDVKELPANVRRGMHFVYVDSMDQVLEVALAKRGTARRATASPAERRAAVRA"
     gene            complement(3244..3513)
                     /locus_tag="AC16G0_02345"
     CDS             complement(3244..3513)
                     /locus_tag="AC16G0_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098490.1"
                     /translation="MTQRPPPRHPQTSDPDHGGGVTPRQDEPETEDSAQGPVEPGEEP
                     DPYDDLWDPDMDTRPERIRKTKARSRTESRTRGGPPPRGKRREGA"
     gene            complement(3551..4423)
                     /gene="galU"
                     /locus_tag="AC16G0_02350"
     CDS             complement(3551..4423)
                     /gene="galU"
                     /locus_tag="AC16G0_02350"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HIR5098491.1"
                     /translation="MTIRKAVFPAAGLGTRFLPATKAQPKEMLPIVDKPTIQYVMEEA
                     VASGIQEIIVVTGRGKNAIEDHFDRSIELEMMLSRKGRSDLLQVVRRISELAHVYYVR
                     QGEPLGLGHAVLAARELVGDEPFAVLLGDDILTDTTPALQQMIAVHEKHHCSVMCVKR
                     VPKEEVSRYGVIKGRKLEDHLYHIMDMVEKPEVGQAPSDLAVIGRYILTPEVFPILEK
                     TAEDRTGEIQLTNALKGLLKGGRNQIYALEFQGRWHDAGTVLGFLKTTVEFALQRSDI
                     APSFKGFLRGLNLT"
     gene            complement(4436..4828)
                     /locus_tag="AC16G0_02355"
     CDS             complement(4436..4828)
                     /locus_tag="AC16G0_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR5098492.1"
                     /translation="MLLFLLRWAATALAIGVAAQVLPGISVDGPWAAIVAALLLGFVN
                     ATLRPILLLLTLPISVLTLGFFALVVNGAMLALVAAVVKGVHVAGFGSAVLGGVVISL
                     VGSFLTWLLQPSPRVHVTLWHSRSEHRG"
     gene            5534..7759
                     /locus_tag="AC16G0_02360"
     CDS             5534..7759
                     /locus_tag="AC16G0_02360"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR5098493.1"
                     /translation="MAKQYKTLRVVLIKPSKYDDNGYVIRFWRGVLPSNTLNVLHGLT
                     VDIAQRRVLGDIEIRIDTFDETVEKVPVKKIVKWSRRPATKLVVCLVGVQTNQFPRAQ
                     DLGREFRSHGLDVILGGFHTSGTINMLSEQEPDIQELVRESIIVVSGEVEGYWGGILA
                     DILNGRPKPIYSFAQDLKSLVDIEKAPLPVMSPSTMKHFARSSFGTVETSRGCPFTCS
                     FCSIINVQGRTMRERSPEWIVDLIRRNYRKHGIDFYFFTDDNFARKKSWREIFEALIR
                     LRKEEGIKVSFMMQVDLARKPKDFVRLAAEAGCSQVFMGMESVNPQNLKVESKGQNKV
                     EAYREIIREWHEAGIVVHAAYIIGLPFDTKEQVPRDIQYLMDVIQPDQASFFMLTPIP
                     GSQDHVEIKKRGEWLDPDFNKRDSFHATVKHPHMTAEEWTQAYRNAWAAFYGKENLIK
                     ILARWTHNPSVYWSLIFVHMWYKNAALIEKEHPMIAGFFRLKDRLSRRPGFAIDPWPV
                     HLWKRTKDVIHLAWSWILFLKEMEEIWLQTRPRSDREKQFCEEIERIQGEIWRTLKIA
                     EWQRTYNDAKASLPAKARALLDPFEDLASKILLSPKDLDAFLEKWGSLQTRMQELYHR
                     VAGDPGPTKRWLDRLAALHKEAWQNPKVQEWHDSYEGFRDRLPSRLQLLYVKFDAVSN
                     RVVYSRQDLRRVWARTWEHLHGKQLWGIRPSRLAVACVKEVVLTTAFTLRVIASFQPK
                     Y"
     gene            8106..8321
                     /locus_tag="AC16G0_02365"
     CDS             8106..8321
                     /locus_tag="AC16G0_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006790110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HIR5098494.1"
                     /translation="MTGKVKWFNDAKGYGFIERADGDDVFVHYTAIQGSGFRSLSEGQ
                     EVEFEVVDGPKGKQAANVTKAVAAQQQ"
     gene            8535..9425
                     /locus_tag="AC16G0_02370"
     CDS             8535..9425
                     /locus_tag="AC16G0_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5098495.1"
                     /translation="MSKRFGDLVAVDGIDFEVEAGECFGLLGPNGAGKTTIARMVQAV
                     VPVTSGDLLVLGHRVDSEPVAVKRRLGVVPQEENLDPDLTPMENLLVFARYFDIPRAE
                     ARRRAADLLDFVGLTTRAHSPLDELSGGMKRRLLIARALLNQPDLCVLDEPTTGLDPQ
                     TRHLVWQRLRGLKAQGVTQILTTHYMEEAAQLCDRVALLHAGRILRQGRPTDLVRAEI
                     GNEVIEIRGDDALHGKIMALLAGKPLRWERAGDTLYVYCREGRSLLPALSALKPSHLL
                     HRPASLEDLFLTLSGRNLEE"
     gene            9553..10323
                     /locus_tag="AC16G0_02375"
     CDS             9553..10323
                     /locus_tag="AC16G0_02375"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098496.1"
                     /translation="MQAWPTWRAARFWQRNLDVFLKYSLPSLVGPSIGEPLLYLLGLG
                     FGLGTLVGSVGGQRYVEFVAPGMVMSSTMFAAAYECTYGAYVRMIHQKTYEAIITTPL
                     SLEDVVAGDILWGASKGVFNGTVMLLVVSLFGLAPSGWTIAVPLVILLLALLFACLSL
                     MVAALARSFEFFNYYLTLVVTPMFLFCGVFYPLSSLPAWAQGLARFLPLTYAVQAARV
                     LMQGNPGPGTLLPLLWLIPPTIAAFLLASRLIRRRLIH"
     gene            10471..10878
                     /locus_tag="AC16G0_02380"
     CDS             10471..10878
                     /locus_tag="AC16G0_02380"
                     /inference="COORDINATES: protein motif:HMM:NF024093.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5098497.1"
                     /translation="MLWYVGYVVLFVEDFDRALSFYSDKVGFPVRLRAEGYAEFAVEG
                     AKLALLARSRVPKLTGDTQAGRPAPGAHEGAVTILVEDVDRTYRELSGRGVPFLGTPQ
                     DRPWGQRTVYFQDPEGHLIEIGTNLHRPERTGV"
     gene            11027..12079
                     /locus_tag="AC16G0_02385"
     CDS             11027..12079
                     /locus_tag="AC16G0_02385"
                     /inference="COORDINATES: protein motif:HMM:NF014653.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HIR5098498.1"
                     /translation="MRTVRADDLRALVASACEKMGARPEDADQVATCLVWANLCGYDS
                     HGVYRLGQYHEWWQKGLLDPAARPAVVAEKGFSAKVDGRRAFGQIVACYATQVAIAKA
                     KAGGIAVVTAMNSNHMGRLADYAEALKGAGLIGLLSVNDSGAGQSVVPWGGMEPRLST
                     NPIAMGIPGSQGPGILFDFSTSAAASGKVRQFWMRGQPVPPGWLIDSAGSPTSDPAAL
                     FTEPHGFLLPAGGHRGYALSLAVEVLSGILSGAGFANPQPGPEEMNGLFVLALNVAWF
                     MPPDQFLAEVDRLTAYVKSAKPQPGAEPIHIPGERSRIEAARRVQEGIRLDEKTCAVL
                     SEVFTSLGLPAELPSQ"
     gene            complement(12151..13668)
                     /locus_tag="AC16G0_02390"
     CDS             complement(12151..13668)
                     /locus_tag="AC16G0_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013703136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HIR5098499.1"
                     /translation="MKRKPAEAYTELIRREKEIALLDSCASLLDWDHQTYMPPRGTTH
                     RAEQLALLVGLSHEKSTDPVIGELLDEAESGDLGREEGSPVPVNLREIRRTYDRTRKL
                     PRSLVEELARTTALAHDVWVEARRESDFAVFEPWLEKVVALKRQEAEAVGYSGVPYDA
                     LLDHYEPGETTAKLIGLFAELRDELVRLVEAIAASGRQPNLSILQRAYPVEQQEDLGM
                     AASAAIGFDFTAGRLDVTTHPFCTGIGPGDTRITTRYNPRDFGDAFFSILHETGHALY
                     DQGLDPIQYGTPMGSAVSLGIHESQSRLWENFVGRSRDFWVHFFPRTRQAFPAALDDV
                     SLDVFYFAINDVRPSFIRVDADEATYNLHILLRFEVEQLLISGDLKVGDVPGAWSERF
                     RQYLGLTPPDDARGCLQDIHWSGGGIGYFPTYTLGNLYAAQFFERARLDLGDLERQFA
                     RGEFAPLTEWLTTKIHRHGQRYRAGDLVLAVTGRPLSPRPLVDHLRSKYAELYGI"
     gene            complement(13755..13937)
                     /locus_tag="AC16G0_02395"
     CDS             complement(13755..13937)
                     /locus_tag="AC16G0_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098500.1"
                     /translation="MPGRFAAAKQAGATDDELMETLYYAMRGRARAAWSTIKYIDGIE
                     DLNKKYKEPFERDGKK"
     gene            complement(13938..>14135)
                     /locus_tag="AC16G0_02400"
     CDS             complement(13938..>14135)
                     /locus_tag="AC16G0_02400"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR5098501.1"
                     /translation="KRGGAMRRTSPVEAWTLTPKEADMSTAVDTSMKEFAGIFYREGA
                     LDKKTMQLVAMAAMAAAGCTS"
BASE COUNT         2456 a         4567 c         4401 g         2711 t
ORIGIN      
        1 gacaccctgc tcccccaggg cggcgaagcg ctgaaagtcg gtcagctccc catccaccgg
       61 cgtctggtcg aacttgaagt cgccgctgtg gatcaccagc ccggcggggg tctgcaccgc
      121 gacggccacg ccgtcgggaa tgctgtggct gacccggatg aactccagcg tgaaaccgcc
      181 caggtgcagc acgtcgcggg gccggatgcg gcgcaggtcg gcggcctcgt ccaggccgaa
      241 ctcgcggagc ttctcctgca ccaagctgag ggccagggtt gtgccgtgga tgggaaccga
      301 aagttgcggc aacaggtagg gcaggccgcc ggtgtggtcc tcgtggccgt gggtgaggac
      361 gatcccccgg accttctccc gctgctccag caggtaggtc acctcgggga ggaccaggtc
      421 gattcccagc atctcttctt ccgggaacat caggcctgcg tcgatcacca ggatgtcctc
      481 ctcggtctcg aggagagtca tgttcatccc gatttcgccg agtcctccca gagggatgat
      541 gcgcacccac ggttcagtct ggctcaaaac ggattggctc ctccttcaca ctcaagggca
      601 cgctggcaag ccggcagctc gtcaaacgag ggcgtcaggt ggccggattg ccaggctgcc
      661 cgttgccatt caatcacgcg cggaccgccg cccgacgctc ggcaggcgag gccgtggccc
      721 gccgtgccgt gccccgcttg gccagggcga cctccaggac ctgatccatg gaatccacat
      781 acacgaagtg catcccgcgc ctgacgttgg cgggcagctc cttgacgtcc ttgtcgttct
      841 tggcggggac gatgaccgtc ttgatcccca tgcggcgggc cgccagggcc ttctccttga
      901 gtccgccgat gggcaggact ttcccgcgga gcgtgatctc gccggtcatg gccacgtcgc
      961 ggcgcaccgg ggtcttggtc atggccgaga tcagcgccgt ggccatggtg atcccggccg
     1021 agggtccgtc cttggggatc gcccccgccg gcacatgaat atggacgtcg tgcttggcga
     1081 aggtctcctc cttgatgccg agggtggtgc cccgggagcg ggcgtagctc atggcggcct
     1141 gggccgactc cttcatcacg tcccccaagt gccccgtgag ggagaggatc cccttgcccc
     1201 gcatcatggt gcactcgatg tacaggatgt ccccgccggt ctgggtccag gccagacccg
     1261 tggcgacccc gatctcgtcc ttctcctgct ccggctcggg caggaacttg ggcgccccca
     1321 ggaaccggtc gaggctggcc ccggtgacgc gggtgggccc cttctttccc tcggccacat
     1381 cccgtgcaac cttccggcag atcgtcgcca cctcgcgctc caggttgcgc agccccgctt
     1441 ctcgcgtgta gctctggacc gtcttcagga tggcgtcgtc ggtcaggtcc agcagcttct
     1501 tgctgatccc gtgctcctcc agttggcggg gcaggatgta gcgcttggcg atgtgcagct
     1561 tctcctcctc ggtgtagccc gagatctcga tcacctccat ccgatctttc agggcgggga
     1621 tgatgggatc ggccaggttc gccgtcgtga tgaacatcac gttccggagg tcaaaggcaa
     1681 cccccaggta gtgatcggtg aaggtctcgt tctgctccgg atccaggacc tccaggaggg
     1741 ccgccgaggg atcgccccgg aagtccgcgc cgatcttgtc cacctcgtcc atcatgaaga
     1801 ccggattgtt ggtcccggcc tgcttgattc cttggatgat cttgccgggc agggcgccga
     1861 tgtaggtccg gcggtggccg cggatctccg cctcgtcccg gacgcccccc agggagatcc
     1921 ggatgaactt gcgtcccagg gcgcgggcaa tggagcggcc cagggacgtc ttccccacgc
     1981 ccggcggccc cacgaagcac aggatcggac ccttcatctt cttcttcagc ttcttcttca
     2041 gggagcggac cgccaggtac tccaggatcc gctctttgac cttctccagg tcgtaatggt
     2101 ccgcctccag gatcttgtgg gcctgcttga tgctcagctt gtcctgggtc tgcttggccc
     2161 agggcaggtc gatgagccag tccaggtgcg tgcggataac cgaggcctcg gcggcgtccg
     2221 ggtgcatacg ggacagccgt ccgagttggg tcttggcctc ctgggcaacg gccgggggca
     2281 tcttcgcctt ctcgatcttt tgttccagct cctggatctc ctggccccgc tcatccgttt
     2341 cgcccagctc tttctggatg gccttgagct gctcccggag gtaatactcc cgatgggtct
     2401 tgtccatctc ctcccgggcc tgggactgga tcttgtgctg catttccagg acctcgatct
     2461 ccttgcccag cagctcggag aggcgcttca gccgctccac cggatcgaag atctccagga
     2521 tctgctgcgc ctgctccacc ttgaggtcca ggttgctggc gacgagatcc gccagtcttc
     2581 cgggatgctc cagattgttg atgatgacca ggacgtccgg cgacaccccc ttgcccaggt
     2641 tgaccccctt ctggatctgc tccttgaccg agcggatgag ggcctcaacc tccaggccct
     2701 gcggacccgt ttcgggctcc gccacctcgg caatcctcgc ctcgaagcac gggtcttgtc
     2761 gggtgaatcc cagaatcttc gcacgggaga cgccctggac caggatcttg acacggccgt
     2821 cgggcatctt cagcatgcgc atgatcatgg ccaccgtgcc cacgggatgg aggtcctcgg
     2881 ggccggggtc ctcggaatcc gcgtcgcgct gggcgaccag caggatcagc cgctcgcggg
     2941 acagggcctc gtccaccgcc cggatggact tgtcccgccc gacgaagagc ggcagaatca
     3001 tgaatggata gaccacgaca tcccgcaccg gcagcagcgg gatctgggac ggcatcttcc
     3061 gctcgccctg gggctgaggc tgctcgatgc ccacgccggc ttcgggttgg ccctcaagcg
     3121 cctccgcgtc cttgtcgttg gcctgttcgc cgctggcctg ccccgcctcg tgttctttca
     3181 aggctcactc tcctttgtct cacccccgcc gcctgggggc gtcctggtgt cgatgggcga
     3241 tgctcacgcc ccttcccggc gctttccccg aggcggggga ccgcccctgg tgcgggactc
     3301 ggttcgcgac cgcgccttgg tcttgcggat gcgctcgggc cgggtgtcca tgtccggatc
     3361 ccagaggtcg tcgtagggat ccggctcctc gccgggttcg accggtccct gcgcgctgtc
     3421 ctcagtttcc ggctcgtcct ggcggggggt cacacccccc ccatggtccg gatctgacgt
     3481 ctgcggatgg cgtggcggtg gtctctgcgt catgctcccc tgccgttcct ccccgaagat
     3541 ccccgtatgc ttacgtgagg ttcagacccc gcagaaaccc cttgaaactc ggagcgatgt
     3601 ccgaccgctg cagcgcaaat tccacggtgg tcttgaggaa gcccagcacc gtccccgcgt
     3661 cgtgccagcg gccctgaaat tccagggcgt aaatttgatt ccgcccgccc ttgagtaatc
     3721 ccttgagggc gttggtcaat tggatctcgc cggttcgatc ctcggcggtc ttctccagaa
     3781 tcgggaagac ctcgggcgtc aagatgtacc gaccgatgac ggccagatcc gagggggcct
     3841 ggcccacctc tggcttctcg accatatcca tgatgtggta gagatggtcc tccagcttcc
     3901 tccccttgat cacaccatag cgggagactt cctccttcgg cacccgcttc acgcacatca
     3961 cggagcaatg atgcttctcg tgcaccgcga tcatctgctg gagggccggg gtcgtgtccg
     4021 tcaggatatc gtctcccagc aggacggcaa acggttcgtc gcccaccagc tccctggccg
     4081 cgaggaccgc gtggcccagg cccaggggct cgccctggcg aacgtagtag acgtgggcca
     4141 attccgagat ccgccggaca acctgcaaca gatccgaccg gcccttccgg ctgagcatca
     4201 tctccagctc gatggagcgg tcgaagtggt cctcgatggc gttcttgccc cggcccgtta
     4261 cgacgatgat ctcttgaatg ccggaggcca ccgcctcctc catgacatat tgaatcgtgg
     4321 gcttgtctac gatgggaagc atctccttgg gctgggcctt ggtcgccggc aagaaccggg
     4381 tcccgagacc ggccgcggga aagaccgcct tccggatcgt cacgtggctt cctccctagc
     4441 ctcggtgttc gctgcgggaa tgccagaggg tcacatgcac gcgggggctc ggctggagca
     4501 gccaggtcag gaagctgccc accaggctga tcacgacgcc ccccaagact gcgcttccga
     4561 aacccgccac gtggactccc ttgaccacag ccgccaccag ggccagcatc gcgccgttca
     4621 ccacaagtgc aaaaaagccc aaggtcagga ctgatatggg cagtgtcagg agcagcagga
     4681 tcgggcgcag ggtggcgttg acgaaaccga gcaagagtgc cgccacgatg gctgcccatg
     4741 ggccgtccac ggaaatgccg ggcagcactt gggccgccac cccgatggcc agtgctgtag
     4801 ccgcccagcg caagagaaac aagagcatca gtcactccaa gtaattcagc aggttacctt
     4861 tataccaact tagcgccact tgtcaaccgc gttcgccccg cagggctgac ggctgatggc
     4921 tcatgctcca tcataaatac cactcgcatg agcgatcagc cacccgtcat ccctgaaatt
     4981 gcgattgaca ttcgggggag tccgcgatag ggtgctcagg aattttcgtc cctgcctcag
     5041 cccgaatgcc tgccttccgg atttccgcag ggtcaggcct cccatcgagc cagagacgcg
     5101 atcgcggcct ctgcagggga acgagacacg cccaatgttc gtcgcaattc accatctcgt
     5161 tactgccatc gtgactgccg cgtgatcctc caatgcctga tgccggtgcg tccatgcatc
     5221 acctgggagg ctcgcggttg gagcaccccg cgcgggaccg cacagacctc accaccgttg
     5281 gaatgcggca ggtcatgagt gccgccatgg tccggatggt tggaagatcg aagctcgcta
     5341 tccagcagac gggcgatcct gatacccgat tccgccacgc tggagtggcc cacacagcaa
     5401 cccaactcac tcggagagtt tcgggggata agtgatgggg aacaacagcc gcacccgcgg
     5461 tgacatgtca ccgcttgccg ttatagactc cgcatctcaa ggctcggaag gatcgtcgtt
     5521 ggagttgctc ccggtggcca agcaatacaa gaccctacgg gttgtgctca tcaaaccgtc
     5581 aaagtatgac gacaacgggt atgtcatcag gttctggaga ggggtactcc ccagcaacac
     5641 cttgaacgtg ctgcacggtc tgaccgtgga catcgcgcag cggcgcgtcc tgggggacat
     5701 cgaaatccgg atcgacacct tcgacgagac ggtcgagaag gtccccgtca agaagatcgt
     5761 caaatggtcc cggcggcccg ccaccaagct ggtggtgtgc ctggtcgggg tccagaccaa
     5821 ccaattcccc agagcccaag acctggggcg ggagttccgg tcgcatgggc tcgacgtcat
     5881 cctggggggg ttccacacga gtggaaccat caacatgctg agcgagcagg agccggacat
     5941 ccaggagttg gtccgggagt ccatcatcgt cgtctccggc gaggtggagg gatactgggg
     6001 ggggatcctg gccgatatcc tcaacggacg gccgaagccc atctactcct tcgcccaaga
     6061 cctcaagagc ctggtggaca tcgagaaagc cccccttccc gtgatgagtc cctcgaccat
     6121 gaagcacttc gcgcggtctt ccttcgggac cgtggaaacg tctcggggct gccccttcac
     6181 ctgctccttc tgctcgatca tcaacgtgca aggccggacc atgcgggagc gctccccgga
     6241 gtggatcgtc gatctcatcc ggcggaacta ccgcaagcac gggatcgact tctacttctt
     6301 caccgacgac aactttgccc ggaagaagtc ctggcgggag atcttcgaag cgctgattcg
     6361 cctgcggaag gaggagggca tcaaggtctc cttcatgatg caagtggatc tggcgcggaa
     6421 acccaaggac tttgtccgcc tggcagcgga ggccgggtgc tctcaggtct tcatgggcat
     6481 ggagagcgtg aatccccaga acctcaaggt ggagagcaaa gggcagaaca aggtcgaggc
     6541 ctaccgggaa atcattcgcg agtggcacga ggccggcatc gtcgttcacg ccgcctacat
     6601 catcgggctc cccttcgaca ccaaggagca ggttccccgg gacatccagt acctcatgga
     6661 cgtgatccag ccggaccagg cctctttctt catgctgacc ccgattccgg gatcccagga
     6721 tcacgtggag atcaagaagc ggggcgagtg gctggatccg gacttcaaca agcgggattc
     6781 gttccacgca acggtcaagc atccgcacat gaccgccgag gagtggactc aggcctaccg
     6841 gaatgcctgg gcggccttct atggcaaaga gaatctcatc aagatcctgg ctcgctggac
     6901 ccacaaccca tcggtctact ggagcctgat cttcgtacac atgtggtaca agaatgctgc
     6961 cctgatcgag aaagagcacc ccatgatcgc cggcttcttc cggctcaagg accggctgtc
     7021 ccgccgcccc ggattcgcca tcgacccgtg gccggtgcac ctgtggaagc gcaccaagga
     7081 cgttatccac ctggcgtggt cttggattct gttcctcaag gagatggagg agatctggct
     7141 ccagacgcgg ccgcggagcg accgggagaa gcaattctgc gaggagatcg agcggatcca
     7201 gggcgagatc tggcggaccc tgaagattgc cgaatggcaa aggacctata acgacgccaa
     7261 ggcctcgctc cccgccaagg cacgagccct gctggatccc ttcgaggatc tggcctccaa
     7321 gatcctgctg agccccaagg acctggatgc gttcctggaa aaatggggga gcctgcagac
     7381 ccggatgcag gaactctacc accgtgtggc gggcgatccg ggaccgacga agcgctggct
     7441 ggatcgcctg gcagccctcc acaaggaggc ctggcagaat ccgaaggtgc aagagtggca
     7501 cgactcctac gagggtttcc gggaccgctt gccatcaagg ctgcagctcc tgtacgtcaa
     7561 attcgatgcc gtgagcaacc gggtggtcta ctctcgtcag gacctccgcc gggtctgggc
     7621 caggacctgg gagcacttgc acggaaagca gctctggggc atccggccct cgcggctggc
     7681 ggtggcttgc gtcaaggaag tcgtcttgac caccgccttc acgctgcggg tcatcgcctc
     7741 gttccagccc aagtattgac atcctgtctc aaccgtgagg caagcgctcg aaagattctg
     7801 gttgtcgcaa taatacaaat tccccggttc tttgaaaaaa ttcttgacaa agactctatc
     7861 ccacgttact gtccgccgcg attgaacaat cgcgtaatac cagatagatg ctcgggatgg
     7921 gcggtttgca ttctgcaagg tgttcaccag tcgcgatcgc gaattgaatt cgccgtcgcg
     7981 cttttttatt tgagcccatc cccgttttat ggggtttggt tgggggggtg cgggtagtct
     8041 ggcccggaag gccctctcgt ttgacgatca tgcgcggaac gaaagaagga ggaccggaag
     8101 tgagactgac tgggaaagtg aagtggttca acgacgcgaa ggggtacgga ttcatcgagc
     8161 gggccgatgg cgacgatgtc tttgtacatt ataccgccat tcaaggaagc ggcttccgct
     8221 ccctcagcga gggccaggag gttgagttcg aggttgtgga tggtcccaag gggaagcagg
     8281 cggcaaacgt caccaaggcg gtcgccgccc agcagcagta gcccaccaga atccgccccg
     8341 agcggattga cgccaaaccc ccggcgacgt accatcgcgc cgggggtttc ttttacggcc
     8401 aaggctgctc aggtccggta actgttcccg cctggccgag catcccgtgg gtgctgaggc
     8461 ggcaactccc tgccggacct ctcggtcctc ggttgcagat tcccatgtct ccacccgcca
     8521 tcactgcccg tgacttgagc aagcgcttcg gggatctcgt cgctgtggat gggatcgatt
     8581 tcgaggtgga ggccggcgag tgcttcggcc tcctcggccc caacggcgcg ggaaagacga
     8641 ccatcgcccg gatggtccag gcggtcgtcc ccgtcaccag cggcgacctc ctggtcctgg
     8701 ggcatcgcgt ggacagcgag ccggttgccg tcaagcgtcg actcggagtc gtcccccagg
     8761 aggagaacct ggacccggac ctcaccccga tggaaaacct gctcgtcttc gcccgctact
     8821 tcgacattcc ccgggctgag gccaggcgcc gtgccgcaga cctcctggat ttcgtggggc
     8881 tgaccacccg cgcccacagc ccgttggacg agctgtccgg tgggatgaag cgccgcctcc
     8941 tcatcgcgcg ggccctgctc aaccagcccg acctatgcgt cctggacgag cccacgactg
     9001 gcctggatcc gcaaacccga cacctcgtgt ggcagaggct gcggggactg aaggcccagg
     9061 gggtcacgca gatcctgacg acgcactaca tggaggaggc cgcccagctc tgcgaccgcg
     9121 tcgccctgct ccacgccggt cgcatcctgc gacagggacg gcccacggac ctggtgcggg
     9181 ccgagatcgg caacgaggtg atcgagatcc gcggggatga cgcgttgcat gggaagatta
     9241 tggcactctt ggccggaaag ccgctccgct gggaaagggc cggagacacc ctgtacgtgt
     9301 attgccgcga gggccgcagc ctcttgccgg cgctgagcgc gctgaagccg tcccacctct
     9361 tgcatcggcc ggccagcctg gaggacctgt tcctcaccct ctccggacgg aacttggaag
     9421 agtagagaca taggcatcta tcatggatcc gcagattacg cagatttcgc agattcccga
     9481 aatccgattc cctaccccaa aaatcgcgta atctgcgata tctgcggata atgagatccg
     9541 gaggttctcc gaatgcaggc atggccgacg tggcgggctg cgcgtttctg gcagcgaaac
     9601 ctggacgtgt tcctgaagta ctcgctgccg agcctggtgg gtccgagcat cggagaacca
     9661 ctgctctacc tccttgggct gggcttcggg ctcgggacgt tggtgggcag cgtgggggga
     9721 caacgctacg tggagttcgt ggctccgggc atggtcatgt cttcgacaat gttcgccgcg
     9781 gcctacgagt gcacctatgg cgcctacgtc cggatgatcc accagaagac ctacgaggcc
     9841 atcatcacca ccccgctgag cctggaagat gtggtggcgg gggatatcct gtggggggcc
     9901 agcaagggtg tgttcaacgg aactgtgatg cttctggtcg tcagcctgtt cggtctggcg
     9961 ccatcgggct ggacgatcgc cgtccccctg gtcatcctgc tgctggctct gttgttcgcc
    10021 tgtctctccc tgatggtggc cgcgctcgcc agaagcttcg agtttttcaa ctactatctc
    10081 acgctggtgg ttacccccat gttcctcttc tgcggggttt tttatcctct cagctctctc
    10141 ccggcttggg cccaggggct cgcccggttc ttgccgctga cgtacgccgt gcaggcagct
    10201 cgagtcttga tgcaaggcaa cccaggcccc ggcaccctcc ttccgctctt gtggctgatc
    10261 cctcccacca tcgccgcctt cctgcttgcg tctcgtctga ttcgtcgccg tctcattcat
    10321 tagcccgtgg actacgattc ggagcgctat ccatcaggca tcacccacaa ccagggtgct
    10381 cttgacatac cggtcaaggt ggggtatcca gtctgaaccc ttgcaggatt ctgcgtgcgg
    10441 cgacaatgac acagtcaaga ggaggaggcg atgctctggt acgtagggta tgtcgtgctg
    10501 ttcgtggagg actttgacag agcgctgtcg ttctattcgg acaaggtcgg gtttccggtg
    10561 cgacttcgag ccgagggtta cgccgagttt gcggtcgaag gggcgaaact tgcgttgctg
    10621 gcacgatcac gagtgccgaa attgacaggc gacacccagg caggtcgacc ggcacctggg
    10681 gcgcatgagg gcgcggtgac catcctggtg gaggatgtgg atcgcaccta tcgcgagcta
    10741 tccggacgcg gggttccctt ccttggcact ccccaggatc gcccttgggg gcagcggacc
    10801 gtctatttcc aggatcccga aggtcacttg atcgagatcg gaacaaacct ccaccgcccc
    10861 gagcgtaccg gcgtctaatt ctctggccga ctccgattga aaccacagat ttcgcagatt
    10921 actcagatgc atccatccga aggcccgttt ccccgtgaaa tctgcgtaat ctgcgtaatc
    10981 ggcggttaaa atggttcggt tgtctccgga gactgtggag atggggatgc ggaccgtgcg
    11041 agccgatgac ctcagggcgc ttgtggcgag cgcctgtgag aagatgggcg ctcggccgga
    11101 agacgccgat caggtggcga cctgcctggt gtgggcgaat ctgtgcggct atgactcgca
    11161 cggcgtgtac cgcctgggcc agtaccacga gtggtggcag aaggggctgc tcgacccggc
    11221 cgcccgtccg gctgtggtgg ccgagaaagg tttctccgcc aaggtggacg gccggcgcgc
    11281 cttcggccaa atcgtggcct gctatgccac acaagtggcc attgccaagg ccaaggccgg
    11341 cggtatcgcg gtcgtcaccg ccatgaacag caaccacatg ggccgcctgg cggattacgc
    11401 ggaggctctc aagggtgccg gcctcatcgg gctgctcagc gtcaatgact ccggtgccgg
    11461 acagtcggtg gtcccctggg gcgggatgga gccccgactc tccaccaacc cgattgccat
    11521 ggggattccc ggatcccagg ggccgggcat cctcttcgat ttctcgacga gtgccgccgc
    11581 ctccggcaag gtccgccaat tctggatgcg aggtcagccc gtgccgcccg gctggcttat
    11641 cgatagcgct ggctctccca cctctgatcc cgccgcattg ttcaccgagc cgcacgggtt
    11701 cctgctccca gcagggggac accggggcta cgcgctgagc ctggctgtgg aggttttgtc
    11761 gggaatcctc tccggggcag gtttcgccaa cccccagccg ggaccggagg agatgaacgg
    11821 cctcttcgtc ctggccttga acgtcgcctg gttcatgcca ccggatcaat ttctcgccga
    11881 ggtggaccgg ctcacggcct acgtcaagag tgcgaaaccc cagccaggag cagagccgat
    11941 ccacatcccc ggcgagcgga gccggatcga agccgctcga cgcgtccagg agggcattcg
    12001 actggacgag aagacctgcg cggtgctgtc cgaggtcttc acgagtctcg ggctgcctgc
    12061 agaactgcca tcccaataat cgacgcggat cggcggcagc ggcagtaatt tgatgccgcg
    12121 gtcggagatt gttctcggcc ctcgtagtcg tcagattccg taaagctctg cgtacttgga
    12181 ccggagatgg tccacgaggg ggcggggact caggggcctg ccggtgaccg ccaagaccag
    12241 gtctccggcg cggtagcgct ggccgtgccg gtggatcttg gtcgtgagcc actccgtgag
    12301 cggggcaaat tcgccccgcg cgaactgcct ctccagatcc cccaggtcca gccgagcccg
    12361 ctcgaagaac tgtgccgcat acaggttccc cagagtgtac gtggggaaat agccgatccc
    12421 acccccactc cagtggatgt cttggaggca gccccgcgcg tcgtccggcg gcgtgagtcc
    12481 caagtactgc ctgaagcgct cgctccacgc ccctggaacg tcgccgacct tcagatctcc
    12541 gctgatgagt agctgctcca cctcgaaccg caagaggatg tggagattgt acgtcgcctc
    12601 atcggcgtcc acccggatga aggacggtcg aacgtcgttg atggcaaagt agaagacgtc
    12661 caggctcacg tcgtccaggg cggcgggaaa cgcctggcgc gttcgaggga aaaagtgcac
    12721 ccagaagtct cggctccgcc ccacgaagtt ctcccacaag cgggactgtg actcgtggat
    12781 gcccagggag acggcgctgc ccatgggcgt cccgtactgg atgggatcca gtccttggtc
    12841 gtagagagcg tgtccggtct cgtgcaggat gctgaagaag gcgtccccga aatcgcgagg
    12901 gttgtaccgg gtcgtgattc gcgtatcccc gggcccgata ccggtgcaaa agggatgggt
    12961 cgtcacgtcc agcctcccgg cagtgaaatc gaacccgatg gcggccgacg cggccatgcc
    13021 caagtcctcc tgctgctcca ccggatacgc gcgctgcagg atcgagagat tgggctgcct
    13081 ccctgatgcg gcgatggctt cgaccagccg gaccaattcg tcccggagtt cggcgaaaag
    13141 cccgatgagt ttggcggtgg tctctccggg ctcgtagtgg tccaggaggg cgtcgtaggg
    13201 gaccccggaa tagccgacgg cttccgcctc ctgtcgcttg agggccacga ccttctccaa
    13261 ccacggctcg aagactgcaa agtccgactc ccgccgcgcc tccacccaga cgtcgtgggc
    13321 cagggccgtg gtgcgcgcca gctcttccac caaggacctg ggaagctttc gggttcgatc
    13381 gtaggtccga cggatctccc tcaagttcac ggggacaggt gatccttcct cgcgacccag
    13441 atcgccgctt tccgcttcgt ccagcagctc accgatcacg gggtcggtgg acttctcatg
    13501 gctgagaccc accaggaggg cgagctgctc cgcccggtgg gtcgtgcccc tcgggggcat
    13561 gtaggtctgg tgatcccagt ctaagagcga ggcgcacgaa tccaagaggg cgatctcctt
    13621 ctcgcgccgg atcagctcgg tataggcctc tgcaggtttt cgcttcatgc atgcccccgg
    13681 aatgattgcc ggatgaacgc cacggcccct tcgccgtgac gaaggggccg tgaggcagcg
    13741 acaaaggcaa ggaactactt cttgccgtcc cgctcgaagg gctccttgta cttcttgttc
    13801 agatcctcga tcccatcgat gtacttgatg gtgctccacg cggcgcgggc cctaccccgc
    13861 atggcgtagt acagggtctc catgagctcg tcatcggtgg cgccggcttg cttcgcggcc
    13921 gcgaagcgtc ccggcactca ggatgtgcag ccggccgccg ccatggcggc catcgccacc
    13981 aactgcatgg ttttcttgtc cagggccccc tcccgataga agatgcccgc aaactccttc
    14041 atgctcgtgt ccaccgccgt gctcatatcc gcctccttcg gtgttagggt ccacgcctca
    14101 accggcgagg ttcggcgcat cgcgcctccc cgctt
//
LOCUS       DBNGZC010000126         4687 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_596960, whole genome shotgun sequence.
ACCESSION   DBNGZC010000126 DBNGZC010000000
VERSION     DBNGZC010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4687)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_596960"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="AC16G0_02405"
     CDS             complement(<1..203)
                     /locus_tag="AC16G0_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098502.1"
                     /translation="MHGLLTVYRKELEDHFSSTRFTLLLTVILMISLITAYMVGSSLR
                     KELEGIAKPSLVFLMLFTSTGAL"
     gene            complement(299..1057)
                     /locus_tag="AC16G0_02410"
     CDS             complement(299..1057)
                     /locus_tag="AC16G0_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5098503.1"
                     /translation="MENVIETHELTKRYGDQVAVNRLNLQVVKGEIFGFLGPNGAGKT
                     TTLLMLLGLSEPTSGTARVCGFDPTREALKVKRVVGNLPENVGFYEDMTAVQNLRFVG
                     RLNGIRDGGLAGKIEEALKAVGLEPEAKKRVGAYSRGMRQRLGIAEVLLKDPQVVFLD
                     EPTVGLDPDGTQKMLELIQALSREKGITVFFSSHLLDQVQKISDRVGIMLKGTLVAAG
                     PIQELAKQKLGVDQAHYTLEELYMRYFHEGEAAA"
     gene            complement(1066..2334)
                     /locus_tag="AC16G0_02415"
     CDS             complement(1066..2334)
                     /locus_tag="AC16G0_02415"
                     /inference="COORDINATES: protein motif:HMM:NF022100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COG1470 family protein"
                     /protein_id="HIR5098504.1"
                     /translation="MTMLARVVQTRRAGGRRREPLGQHAMFAAVLLAVLALTAPGVGW
                     GAEDQTKRDDRPERGISIYTDYSGVVVPVGDSVRMDLTVDNRGKQDETVALKLVNVPK
                     GWKASLKGGNFTITGVSVASGKTRTLAFSAEPEKGVGPGTYAFDIEGVSADGKLTAKQ
                     PITITARERSKLGGGDIQVSTSYPVLRGQNDAQFEFSLDVANKSENDRTFNLAAQVPE
                     NWEVNFKPGFESKQISSLQIRGGASQTVAVQVTPARGALAGEYPVLFRVTSGESKSET
                     KLTVALTGTYKLDAATTNGVLSADAVTGKPTTVSFLVKNTGSAVNHNISLSAFKPENW
                     KVEFKPEKLESLEPGAFKQIEATITPSATALVGDYSVGLSADGEKGSSKSVELRVTVK
                     APTAWGWFGVGLIAVVIGGMGGLFVWLGRR"
     gene            complement(2536..2922)
                     /locus_tag="AC16G0_02420"
     CDS             complement(2536..2922)
                     /locus_tag="AC16G0_02420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5098505.1"
                     /translation="MEIRLFLVDDDSGFRETLRQLLTQRKEAIILGEAGDGEEALRLT
                     STLRPDVVLMDLAMPRMNGLEATRHLKALRSDLVVIILTVHDDEAYRRTALAAGAEAF
                     LVKKTLGVDLWPTLERVLNRRQGQPS"
     gene            complement(3132..3710)
                     /locus_tag="AC16G0_02425"
     CDS             complement(3132..3710)
                     /locus_tag="AC16G0_02425"
                     /inference="COORDINATES: protein motif:HMM:NF014025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HIR5098506.1"
                     /translation="MRESLQAFVALFVAVDILGLLPIYLSLTGNLPADKRRRLPALAT
                     LTAMAIGLGFMLIGRALLRIMGVSVGDFQVAGGILLLILSIQDLLHPGHVLRRPGPTI
                     GAVPLGTPLIVGPAVLTALVALEQSYGPGITLGAFAVNMGVVFLALRNVDLVGRILGE
                     AGSQAVAKVASLFLAAFGVSLVRRGLPGFLGR"
     gene            complement(3783..4415)
                     /locus_tag="AC16G0_02430"
     CDS             complement(3783..4415)
                     /locus_tag="AC16G0_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5098507.1"
                     /translation="MIRVLLADDHTIVREGLRALLAKETDFQVVGEAGDGHEALRLAK
                     KCRPDVAAMDLSMPLLNGLEAARQMAAWEQGPRTILLTMHAEDSYVLEALRAGVRGYV
                     LKKQAGADLVRAIREVVAGGVYLSPGVSATVAGAIRSPGPLPEESLTAREREVLQLVA
                     EGKTTKEIGTILGVSTKTADAHRTRLMQKLDIHDIAGLTRYAIRSGLIQP"
     gene            complement(4412..>4687)
                     /locus_tag="AC16G0_02435"
     CDS             complement(4412..>4687)
                     /locus_tag="AC16G0_02435"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5098508.1"
                     /translation="VQEALTNAARHAHATRVQVEVQLAGSAVRVVVQDDGQGFDVDAA
                     QARRGDRGLGLIGMRERVDALGGGLSIVSGRGQGTTLSVVIPLGGAA"
BASE COUNT          789 a         1645 c         1420 g          833 t
ORIGIN      
        1 aagagcgccc ccgtggaggt aaagagcatc aagaacacca gcgaaggctt ggcgatcccc
       61 tccagttcct tccgcaagct gctccccacc atgtacgccg tgatgaggct gatcatcagg
      121 attaccgtga ggaggagggt gaagcgggtg ctgctgaagt ggtcctccag ctctttgcga
      181 tagacggtca acaatccgtg cacgtctgct ccttccactg gaacctccgg caagtcgaca
      241 gtacgaccgg ccggctgatc agtcggatgc ctctcgttgc accgcgcttc tcccgcattc
      301 aggctgcggc ttcgccctca tggaaatagc gcatgtagag ttcttccagg gtgtagtgtg
      361 cctggtccac ccccagcttt tgcttcgcca gctcctggat gggacccgca gccaccaacg
      421 tccccttgag catgatgccg acgcggtcgc tgatcttctg gacctggtcc agcaggtggg
      481 aggagaagaa gacggtgatc cccttctccc ggctgagcgc ctggatcagc tccagcatct
      541 tctgggtccc gtcgggatcc agtcccaccg tgggttcgtc caggaagacc acttgcgggt
      601 ccttgagcag gacctcggcg atgcccaacc gctggcgcat gccccgcgag taagcgccca
      661 cccgcttctt ggcctcgggc tcgagaccga cggccttcag cgcctcctcg atcttcccgg
      721 ccaggccgcc gtcccggatg ccattcagtc gcccgacgaa cctgaggttc tggacggcgg
      781 tcatgtcctc gtagaacccc acgttctccg gcaggttccc caccacccgc ttgaccttca
      841 gggcctcccg agtgggatcg aagccgcaaa cccgggcagt cccggaagtc ggctccgaca
      901 gccccaggag catgagaagc gtggtcgttt ttccggcccc gttggggccc aggaagccga
      961 agatctcccc tttcacgacc tggaggttca gccggttgac cgccacctgg tccccgtagc
     1021 gcttggtcag ctcgtgagtc tcgatcacgt tctccatgag acgcctcaac gccggccgag
     1081 ccagacgaac aggccgccca ttcccccgat caccacggcg atcaggccga caccgaacca
     1141 accccaggcc gtgggagcct tcaccgtcac ccgcagctcc acgctcttgc tgctgccctt
     1201 ctcgccgtca gccgacaggc ccacggaata atctcccacc agggcggtgg ccgagggcgt
     1261 gatcgtcgcc tcgatctgct tgaacgcccc cggttccagc gactccagct tttcgggttt
     1321 gaactccacc ttccagttct cgggtttgaa tgccgacaga ctgatgttgt ggttgacggc
     1381 ggagccggtg ttcttgacca ggaacgacac cgtcgtgggc ttgcccgtga ccgcgtcggc
     1441 ggacaggaca ccattggtgg tcgccgcatc cagcttgtac gtgcccgtga gggccaccgt
     1501 cagcttggtc tcggacttgg actctccgga tgtcacccgg aaaagcacag gatactcacc
     1561 cgccagcgcc cccctcgcag gggtgacctg gacggccact gtctggctgg cgcccccccg
     1621 gatctgcagg ctggagatct gcttcgactc gaagccgggc ttgaagttga cctcccagtt
     1681 ctccgggacc tgagcagcca ggttgaaggt gcgatcgttc tcactcttgt tggccacgtc
     1741 cagggagaac tcgaactggg cgtcgttctg cccgcggagg acggggtagg acgtgctgac
     1801 ctggatgtcc ccaccgccca gcttgctccg ctcgcgcgcg gtgatcgtga tgggctgctt
     1861 cgccgtcagc ttcccgtccg cgctgacccc ctcgatgtcg aaggcatacg tcccgggacc
     1921 cacacccttc tcaggctcgg cgctgaaggc gagtgtccga gttttcccgc tggcgacgga
     1981 gacgcccgtg atcgtgaagt tgcccccttt gagggaagcc ttccatccct tgggaacatt
     2041 cacgagcttc agggccaccg tctcatcctg cttcccccgg ttgtccaccg tcagatccat
     2101 gcggaccgaa tcacccaccg gcaccacgac cccggagtag tcggtataga tggagatccc
     2161 ccgctccggc cggtcatccc gcttggtttg gtcttcggcc ccccagccaa cgccgggcgc
     2221 ggtcaaggcg agcaccgcga ggagaaccgc cgcgaacatc gcgtgttgtc ccaggggttc
     2281 ccgtcgtcgg ccccccgccc ttcgtgtttg taccacccgc gccagcatcg tcatctctcc
     2341 tctcctgtgt gcggccgggg cgtcccgacc gcttgagctt gcccgatggg atgtcggtcc
     2401 ccgtcacggg cgcgctccgc tgcgacctct ccgctctcct ggaggtctgt gtgtgctgct
     2461 taccccaaca gagcatagac gcctgagggc aatcccgcaa ttgggaaaac atacgattgc
     2521 gccaccgccg cgccttcagg acggttggcc ctgacgcctg ttgagtaccc gctccagcgt
     2581 cggccagagg tccacgccga gcgtcttctt caccagaaat gcctcggctc ccgccgccag
     2641 tgccgtccgg cggtaagcct cgtcgtcgtg caccgtcagg atgatgacga ccaggtcgga
     2701 gcggagggcc ttgaggtgcc gggtggcctc aagaccgttc atccggggca tggcaaggtc
     2761 catcaggacc acgtccggac gcaaagtgct cgtcaggcga agggcctcct cgccgtctcc
     2821 ggcctcgccc aggatgatcg cctccttccg ctgcgtcagg agctgccgga gcgtctcccg
     2881 gaacccgcta tcgtcatcaa ccaggaacag ccgaatttcc attatcgcaa cgcggcctcg
     2941 gtatttccgt acgggcccgc atctgcctgg gaggataggc gggcgcggtg ccaaggggaa
     3001 tagggtgaac tccctagtcg accgggagaa aatccccatg cccctggcga ttcccacgcc
     3061 tccctctcct tggtgtctgc gtgcatctgt cggatacaat ctgcgaaatc tgcggctaag
     3121 gagagttgat ctcatctgcc caggaacccg ggcagcccgc gccggaccag gctcacgccg
     3181 aaggccgcca gaaacaggct ggccaccttg gcgactgcct gggagcctgc ctctcccaag
     3241 atgcgcccca cgagatccac gtttcggagc gccaggaaca ctacccccat attcaccgcg
     3301 aaggccccaa gggtgattcc gggcccgtag ctctgctcca aggccacgag cgcggtcagg
     3361 accgccggac ccacgatcag tggcgtcccc agcgggaccg cgccgatggt cggacccgga
     3421 cgccgcagga cgtgacccgg gtgcaagagg tcctggatcg agaggatgag gagcagaatg
     3481 ccgccggcca cctggaagtc ccccacactc acgcccatga ttcggagcag cgcccggccg
     3541 atcagcatga acccgagtcc gatcgccatc gccgtcaagg tggcgagcgc aggcaggcgt
     3601 ctccgtttat ctgccgggag gttgcccgtc aagctcaggt aaatgggaag aagacccagg
     3661 atgtccaccg ccacgaacaa ggccacaaac gcctgcaagg attctcgcat gtcgccctcc
     3721 tgggtgcggc agtgtatctg ggcagggtca ttgcgtgctt ggaagtgaga agctcagggc
     3781 gctcacggct ggatcaggcc ggaacggatc gcgtaacggg tgaggccagc gatgtcgtgg
     3841 atgtccagct tttgcatcag gcgggtccgg tgcgcgtcgg cggttttcgt gctgacgccc
     3901 agaatggtgc caatctcctt ggtggtcttc ccctccgcca ccaactggag gacctcccgt
     3961 tcgcgggcgg tcagggattc ctcgggcagc gggccggggg aacggatggc cccggcgacg
     4021 gtggcagaca cccccggact gaggtaaaca ccgccggcta ccacctcgcg gatggcccgg
     4081 accagatccg ccccggcttg cttcttcagc acatatcccc ggaccccagc ccgcaacgcc
     4141 tccagcacgt agctgtcctc cgcgtgcatg gtgaggagga ttgtccgcgg cccctgctcc
     4201 caggcggcca tctggcgcgc cgcctccagg ccgttcaaga gcggcatgga gaggtccatg
     4261 gccgccacat ccgggcggca tttcttggcc agccggaggg cctcgtggcc gtcaccggcc
     4321 tcgcccacga cctggaaatc ggtctccttc gccaggaggg cccgcagccc ctcgcggacg
     4381 atggtgtgat cgtcggcgag cagaacccgg atcatgccgc gcccccgagg ggaatcacga
     4441 cggagagcgt tgtcccctga ccgcgaccgg agacgatgga gagcccaccg cccagagcat
     4501 ccacccgctc ccgcatcccg atgaggccca acccccgatc tcctcgccgg gcttgagcgg
     4561 catcgacatc aaagccctgg ccgtcgtcct ggaccaccac ccgcacggcc gatcccgcaa
     4621 gctggacctc cacctgcacc cgcgtcgcat gcgcgtgccg tgccgcgttg gtgagcgctt
     4681 cctggac
//
LOCUS       DBNGZC010000127         2623 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2456102, whole genome shotgun sequence.
ACCESSION   DBNGZC010000127 DBNGZC010000000
VERSION     DBNGZC010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2456102"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="AC16G0_02440"
     CDS             <1..452
                     /locus_tag="AC16G0_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016970482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098509.1"
                     /translation="RYLKDREVAEAVQGYLQAVGVQAEVRVWEWSPYLTFIQKDESRE
                     AFMVGRATPSADFTATRLFSKSAIGLYNVTGFWREKIEDLLVKGRSSFDEKERTDNYR
                     EIQAIVWEEAPWIFLHNQKAVVGVRKNIDGYAMLTTEVSLLGNVSKR"
     gene            517..825
                     /locus_tag="AC16G0_02445"
     CDS             517..825
                     /locus_tag="AC16G0_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006965284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR5098510.1"
                     /translation="MKRREDSSLRLDANVDRGRPVQISVDGESIQAYEGESLAAALLA
                     AGRRVFRHAEPGGDPRGIFCGMGICYDCLVTVTGQDHVRACMTLVQEGMQVSTTTMVE
                     "
     gene            895..2298
                     /locus_tag="AC16G0_02450"
     CDS             895..2298
                     /locus_tag="AC16G0_02450"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5098511.1"
                     /translation="MREVEIVIVGAGPGGLGAALEATEAGAEVILIDEYPTLGGQFYK
                     QVPDAFRLKRLEAESEQYVEGAELIQRVKASRIEFLSNTLVWSAFRDGTLGLYRDGRT
                     EELRGKRLILAPGAQEVPVAFPGWTLPGVMMGGAAQSLLVTHRVLPARRVVLAGVGPL
                     QLKVACQLLEAGATVVDILEASGTPPVSMENALRSLGHWGKMREGMKYWLRLKKSGTP
                     YHHRHVPVRALGTDQLEAVVVAQVNDEWQVVSGTERTLEADSLSLSYGFVPSTQLTRL
                     LGCRMEYNGRTGGWVTWHDADQQTSFAGVYVAGEVGGIGGADVALEEGRIAGLSAARA
                     LGRVTAGGRQKEEREARKRLSRAWEFADVAAGMMQLKPALIDLATDETILCRCENVQV
                     KRVMEAIGFEGDSTLRGVKIHTRAGMGPCQGRICSYLISRLIAKKTGVPLESIQPDTA
                     QAPIKPVPLRALVAHVQ"
BASE COUNT          486 a          753 c          940 g          444 t
ORIGIN      
        1 gacggtatct gaaagaccgg gaggtggcgg aagctgtcca gggctatctg caggcggtgg
       61 gggtgcaggc ggaggtccgg gtctgggagt ggagcccgta cctcacgttc atccagaagg
      121 acgagtcgcg cgaggccttc atggtcggcc gggcgacgcc ctctgcggac ttcaccgcaa
      181 cccggctctt cagcaagagt gccatcggtc tctacaacgt gaccggattc tggcgggaaa
      241 agatcgagga tctgctggtc aagggccggt cgtcgttcga cgagaaggag cgcaccgaca
      301 actaccgcga aatccaggcc atcgtgtggg aggaagctcc ctggatcttc ctgcacaacc
      361 agaaggccgt ggttggagtg cgcaagaaca tcgacggcta cgccatgctc accaccgagg
      421 tgagcctcct gggcaatgtg tccaagcggt gaaagggcgg tgggcgggcg cgacatcgcg
      481 gtcccgcccg cagccccagc caaggggttg tgtgacatga agagacggga ggacagttcg
      541 ttgcgcctgg acgccaatgt ggaccgcggc cgcccggtgc agatcagcgt ggacggagaa
      601 tccatccagg catacgaggg cgagtcgctg gcggctgccc tgctggcggc cggccggcgg
      661 gtcttccggc atgccgagcc gggaggggat ccgcggggga tcttctgcgg catggggatc
      721 tgctatgact gcctggtgac ggtgaccggc caggatcatg tccgggcctg catgaccctg
      781 gtgcaggagg ggatgcaggt ctcaactaca accatggtcg aatgaccaat gacaaatgac
      841 caatgagatc cgggctcgtc attggtcatt ggttcagtaa gggaggcggc gacggtgcga
      901 gaggtcgaga tcgtcatcgt gggcgcaggt ccagggggtc tgggcgccgc gctggaagca
      961 acggaggccg gggccgaggt gatcctgatc gacgaatacc ccaccctggg cggccagttc
     1021 tacaaacaag ttcccgacgc ctttcgcctg aagcggctgg aggcggaaag cgagcagtac
     1081 gtcgaagggg ccgagctcat ccaaagggtg aaggcctcca ggatcgagtt cctctcgaac
     1141 accctcgtct ggtcggcctt ccgggatggc acccttggcc tttaccggga cgggcggacc
     1201 gaggagttgc ggggcaagag gctgatcctg gcgccggggg cacaagaagt gcctgtggcg
     1261 ttccctggat ggacgctgcc gggtgtgatg atggggggcg ccgcccagtc cctcctggtg
     1321 acgcaccgcg tgttgcctgc gcgccgggtg gtgctggcag gggtcggacc cctgcagttg
     1381 aaggtcgcgt gccagttgct cgaggccggg gcgaccgtcg tggacatcct ggaggcctcc
     1441 ggcaccccgc cggtttccat ggagaacgcg ctcaggtccc tggggcactg gggaaagatg
     1501 cgggagggaa tgaagtactg gttgaggctg aagaagtccg gcacccccta ccatcaccgt
     1561 cacgtccccg tccgcgccct gggcacggac cagctcgagg ccgtggtggt cgcccaggtg
     1621 aacgacgagt ggcaggtcgt gtccggaacc gagcggacac tggaggcgga tagcctgtcc
     1681 ctctcgtatg ggtttgtgcc ctccacgcaa ttgacccgac tcctggggtg ccggatggag
     1741 tacaacggac gcacaggcgg atgggtgacc tggcatgacg ccgaccagca gaccagcttc
     1801 gccggcgtct atgtcgccgg agaggtcgga ggaatcggcg gcgccgatgt ggccctagag
     1861 gaggggagga tagccggcct ttcggccgca cgcgcgctgg gccgtgtgac cgccggtggc
     1921 cggcaaaagg aggagcgaga ggcccgcaag cggctgtccc gagcgtggga attcgcggac
     1981 gtggccgccg gcatgatgca gctcaagcct gccctgatcg acctggcaac cgacgagacg
     2041 atcctgtgcc ggtgtgagaa cgtgcaggtc aagcgggtga tggaggcgat aggcttcgag
     2101 ggcgattcta ccctccgggg cgtgaagatc cacactcgag cggggatggg gccgtgtcag
     2161 gggcgcatat gcagctacct gatctcccgc ttgatcgcca agaagacggg tgtgcctctg
     2221 gagtccatcc agccggatac cgcccaggcg cccataaagc cggtcccatt gcgggcgctg
     2281 gtggctcatg tgcaatgagc gggctcacgg gcccacctgc aagtttgctc agatctctcg
     2341 ggtcgctgta ggccgagacg acgagaactg ctcggaccaa gagcaaccac acaattattc
     2401 ggaggctgac ctggattatt ctcgaatgga ctcgtgattt ctccggatca aatgaccaat
     2461 ggccaatttc caatgaccaa tgccggtggg cgccgtcacg gagcatcccg gagggggatc
     2521 ccgccattgg tcattggcca ttgggatttg gtcattcccg cgatcattcg cgagctcgtt
     2581 cgcgaatgat ccgggccaag ggctggtgaa gggaggactt cat
//
LOCUS       DBNGZC010000128         1380 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1086345, whole genome shotgun sequence.
ACCESSION   DBNGZC010000128 DBNGZC010000000
VERSION     DBNGZC010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1380)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1380)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1380
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1086345"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            361..>1380
                     /locus_tag="AC16G0_02455"
     CDS             361..>1380
                     /locus_tag="AC16G0_02455"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098512.1"
                     /translation="MSQRKGERGAWIWGIVFTLCWVVFPTGAMAGEAKPGGTVKLGQV
                     GGVLNFDAHRLSRGNYPMLTQVYNTLVRYDDNLKVHPDLAESWSYSQDGLTLEFKLRQ
                     GVKFHNGREFVADDVVWNIQRVQDPKTAAHVRPLSLSIKSVEAPDTYRVVVRTEQPTP
                     ALMDLFETMFILPKEAAGDIQKRGVGTGPFQVVEWVPGDKVVLKKFPEYFRSGLPYLD
                     GVILQQIPDAPALAVNLEAGALDVAWELPPREAARLMNTRQAMLLQGMGGASVTDIMM
                     NASKAPFDNKLVRQAINHAVDRKRFVDTVLVGFGEPWCQPFPAQSLGFSKELGTKHCT
                     FDLARA"
BASE COUNT          238 a          425 c          480 g          237 t
ORIGIN      
        1 accgcttcct ctcccttgat tgtctgtctt tctccttgac agggtggctc ggcccgcggc
       61 accatcggcg gcgtcgtgtg cctcgggtcc cctggggaga tgcgcacggc gagaggcgcg
      121 ggttcctgcc gtctcgcacg aggtaggctc ccgcgtccgg ggcgtgtccg agcaggccgc
      181 tgaaaaaggc ccatctgccg ggtacccatt ccgatgggtg cccatcctgg cggacgggta
      241 cccggcgtgc cgccttgcat ctggaccttt ttgagcagcc tggcgagaag cgaatttttc
      301 ggcaacctgc cagagctttc tcgggcgaaa cgcaaaccgt tcgcgtggga ggaaccgagc
      361 atgagccagc gaaagggaga gcgaggtgcc tggatatggg ggatcgtctt caccctgtgc
      421 tgggtggtct tcccgacggg ggcgatggcg ggagaggcga aacctggcgg gacggtgaaa
      481 ctgggacagg tgggcggggt tctgaacttc gatgcgcacc ggctcagccg cgggaactac
      541 ccgatgctga cccaggtcta caacaccctg gtgcgctacg atgacaacct caaggtgcac
      601 ccggatctcg ccgagtcctg gtcctacagc caggacggcc tgacgctcga gttcaagctg
      661 cgccaggggg tcaagttcca caacggccgc gagttcgtgg cggatgatgt ggtctggaac
      721 atccagcggg tccaggatcc caagacggcg gcgcacgtgc gaccgctcag cctgagcatc
      781 aagtccgtgg aggcgccgga tacgtaccgg gtcgtggtcc ggacggagca gccgacgccg
      841 gccctcatgg atctgttcga gaccatgttc atcctgccca aggaggcggc gggagacatc
      901 cagaagcggg gagtcgggac cggccccttc caggtcgtgg agtgggtacc cggggacaag
      961 gtcgtcctga agaagtttcc ggagtacttc cgatcggggc tgccctatct cgacggggtg
     1021 atcctgcagc agattcccga cgccccggcc ctggccgtca acctggaggc gggggcgctg
     1081 gatgtggcat gggagcttcc gccccgggag gcggcgcggc taatgaacac gcgccaggcg
     1141 atgctgctgc agggcatggg tggcgcctcg gtgaccgaca tcatgatgaa cgcctccaag
     1201 gcccccttcg acaacaagct ggtgcggcag gcgatcaacc acgccgtgga ccgaaagcgg
     1261 ttcgtggaca cggtcctggt aggattcggg gaaccatggt gccagccctt cccggcccag
     1321 tccctgggct tttccaagga gctagggacc aagcactgca ccttcgatct ggcccgggcc
//
LOCUS       DBNGZC010000129         1983 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2064741, whole genome shotgun sequence.
ACCESSION   DBNGZC010000129 DBNGZC010000000
VERSION     DBNGZC010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2064741"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="AC16G0_02460"
     CDS             <1..800
                     /locus_tag="AC16G0_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098513.1"
                     /translation="AMLLVALTAGMIAPGSPTALSRDSLQTPGPRHWMGTDDLGRDIL
                     AGVVYGARLSLLVGFLTAGASTLIGICVGGIAGYYGGKLDDLLMRLTEFFMVVPRFFL
                     ALVIVALFGASLWGIILVLTILSWPTTARLFRAEILRLKGQEYVLAARAMGSSDHRIL
                     VGQLLPNALSPVIVSVSVQVAYAIILEAGLSFLGLGDPANRSWGVMLSNAQQFLRQAW
                     WMATFPGLAIFLAVLGFNLIGDGVNDALNPRLRTRGRRLSSPHWEQA"
     gene            827..>1983
                     /locus_tag="AC16G0_02465"
     CDS             827..>1983
                     /locus_tag="AC16G0_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098514.1"
                     /translation="MHTVDVGRLRRTWIGLAAFALMLWMTAAPAWAGAAAEAAKYGGT
                     FVVAIANDPTTLNPATSSDFYVKIISGTVFDTLVNLDFNFNPVPRLAKSWDISKDGLT
                     YTFHLVNTKWQDGRPFTSEDVKFTVEQVLTPFHPSGAQYKDRIQEIQTPDPQTVVFKL
                     KTVFPPFLIMLTNDLYVMPKHIYQGTDILKNPARLTPIGTGPFKFSEWKRGDYIRVVR
                     NPDYFQAGKPYLDQIVFKVAADPGARMLAFEKGELDYLSFYMFPASEVERVRKIPGLK
                     TTTKGHEIFGDILDLFFNLDKTPLNNLKVRQAIAHAVDKNYLLDKADFGLGKVATGPI
                     PSSLPWAYTADVTRYAYDVNQANKLLDEAGLPRKEGGVRFKLSLMWDRTVPL"
BASE COUNT          349 a          633 c          632 g          369 t
ORIGIN      
        1 cggccatgct cctggtcgcg ctgacagccg ggatgatcgc cccggggtcc ccaaccgccc
       61 tcagccgcga ttcgctgcaa acgcccgggc ccaggcactg gatgggcacc gacgacctgg
      121 ggcgcgatat cctcgcgggc gtggtctacg gggcccggct gtctctcctg gtcgggttcc
      181 tgacagcggg cgcgtccacc ctgatcggaa tctgcgtggg cggcattgcc gggtattacg
      241 gagggaaact cgacgacctg ctcatgcgac tgaccgaatt cttcatggtg gtgccgcgct
      301 tcttcctggc gctggtgatc gtggcgctgt tcggcgccag tctctggggc atcatcctgg
      361 tcttgaccat cttgagctgg ccgacgactg cccgtctgtt ccgggcggag atcctgcggc
      421 tgaagggcca ggagtacgtg ctggccgcgc gggccatggg gagcagcgat caccggatcc
      481 tggtcgggca gctcctgccc aatgcccttt cgcccgtgat cgtgagtgtc tcggtgcaag
      541 tggcgtatgc catcattctg gaggccggcc tcagcttcct cggcctcggg gatccggcca
      601 accggagctg gggtgtcatg ctttcaaacg cgcagcagtt cctgcgccag gcctggtgga
      661 tggcgacctt ccctgggctg gcgatcttcc tggcggtgct gggattcaat ctcatcgggg
      721 acggggtgaa cgacgcgctg aatccgcggc tcaggacgcg cggccggcgg ctgagttctc
      781 cccactggga acaggcgtaa cacagacgtg acggatggag gggaccatgc acactgtaga
      841 cgtggggcgc ctcaggcgga cgtggatcgg tctggccgca ttcgctctga tgctctggat
      901 gacggcggcg cccgcgtggg ccggggcggc ggcggaggcg gcgaagtacg gggggacgtt
      961 cgtggtcgcg atcgccaacg atcccaccac gctgaacccg gccacgagca gcgatttcta
     1021 cgtaaagatc atcagcggca ccgtgttcga caccctggtg aacctcgact tcaacttcaa
     1081 tccggttccg cgcctcgcca agtcctggga catctccaag gacggactga cctacacgtt
     1141 ccatctggtc aacaccaagt ggcaggatgg gcgcccgttc acatccgagg atgtgaagtt
     1201 cacggtggaa caagtcttga cgccgttcca cccgagcggc gcccagtaca aggatcgcat
     1261 ccaggagatc cagacgccgg atccccagac ggtggtgttc aagctgaaaa ccgtcttccc
     1321 gccgttcctg atcatgctga ccaacgacct ctacgtcatg cccaagcata tctatcaggg
     1381 caccgacatc ttgaagaacc ccgccagact gaccccgatc gggaccggcc cgttcaagtt
     1441 ctccgagtgg aagcggggcg actacatccg cgtggtccgg aaccccgatt actttcaggc
     1501 cgggaagccg tacctcgacc agattgtttt caaggtcgcg gcggatccgg gggcgcgcat
     1561 gctggccttc gagaagggcg agctggatta cctctccttc tacatgttcc ccgcttccga
     1621 ggtggagcgt gtccgcaaga ttcccggcct gaagacgacg accaagggtc acgaaatctt
     1681 cggggacatc ctggacctgt tcttcaatct cgacaagacc cccttgaaca atctcaaggt
     1741 tcgacaggcg atcgcccatg ccgtcgacaa gaactacctg ctggataagg ccgacttcgg
     1801 cctgggcaag gtggcgacgg gacccatccc cagttcgctg ccctgggcct acaccgcgga
     1861 tgtcaccagg tatgcttacg atgtgaacca ggccaacaag ctgctggacg aggccggcct
     1921 cccccgcaag gagggcggcg tccggttcaa gctgagcctg atgtgggacc ggaccgtccc
     1981 gct
//
LOCUS       DBNGZC010000130         6186 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2554553, whole genome shotgun sequence.
ACCESSION   DBNGZC010000130 DBNGZC010000000
VERSION     DBNGZC010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6186)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6186)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6186
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2554553"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            711..1670
                     /gene="pfkB"
                     /locus_tag="AC16G0_02470"
     CDS             711..1670
                     /gene="pfkB"
                     /locus_tag="AC16G0_02470"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HIR5098515.1"
                     /translation="MDFFPRDNGMVTPIVTVTPSPALDRTLRVDRLDFGAVARVRDVR
                     EDPGGKGVNVSRVLNQFGWPTAALGFIGGSIGRRLEEELHRYGIVTDFVPVGGETRIN
                     LTLTDGERELKVNESGPPVDSTAVGSLIEKVRCHARRSRAVVLAGSLPPGAGEGLYAE
                     LVEVIRQEGSRAVLDAEGTPLRLGVAARPFLVKPNLREAEALLERPLSTEADICQAAR
                     RLGDRGIEVVVISLGGSGAMMARHGRVWMAEIPQTRTVSTVGAGDSLLAYLLMAILQD
                     MDPAASLLRATAAGLASTSLPGSQLCTPPEVERMIPLVRTREL"
     gene            1714..2853
                     /locus_tag="AC16G0_02475"
     CDS             1714..2853
                     /locus_tag="AC16G0_02475"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR5098516.1"
                     /translation="MTGTPGIQSRMMAALLTGAERIQLAEVPRPVCPEDGLLLRVAAC
                     AVCGSDVRLYLGRKRIQGGQEIAGQPLPGHILGHEIAGVIHEVGSSVRGFRPGEAVIV
                     APGITCGKCDACLGRRPVVCRNYQALGYQFPGGFAEYLAVPGCLLQDGSVNRVPEGVP
                     SWKACLAEPLACTLNAQEAMGIRAGDSVLVTGAGPMGCLHLLLARQRGAGFIAVAEPD
                     AGRRERAGRLGADLVVNPDAPNLEQLLLGGTQGEGFTAVIFAVSSIEAVRLLFTAYEA
                     GRYRLVAPGARVNFFAGLDPGETSLSLDARAIHYQGLCLLGSVNSTPRQNTEALRLIA
                     SGAVPVESLVSARLPLSRVGEALTLAMSRSHLKVIVEPGGSEGKA"
     gene            complement(2956..3414)
                     /locus_tag="AC16G0_02480"
     CDS             complement(2956..3414)
                     /locus_tag="AC16G0_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR5098517.1"
                     /translation="MRIEQELEKMGFVLPEPKPLAAYVPAVRSGNLIFTAGQGPTLDG
                     KPKFTGKLGRELTEEQGYQAAQICVVNCLACVKSLIGDLDKVERVVKLLGFVASVDGF
                     TRQPWVMNGASELLIKLFGDRGKHARSAIGTNQLPQGIPVEVEMVVQVRD"
     gene            complement(3640..4368)
                     /locus_tag="AC16G0_02485"
     CDS             complement(3640..4368)
                     /locus_tag="AC16G0_02485"
                     /inference="COORDINATES: protein motif:HMM:NF019413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HIR5098518.1"
                     /translation="MAALTEREHALGMEKQAKRHLIACEVFKDEFEAVSPPDLIRTYL
                     PQGLHRTPGKMPAAIQEVVDALPADTELVILGYGLCSNGVVGVTARTAPILMPKVHDC
                     IALLLGSCERYEAEVAACAGTYYITPGWASYGTTSLSCYKNEYLPKYGEEDARYIAHE
                     LLKNYKRVAFIDHGVGNIDAGRAHAAEMAELFNLAYAEIPGSLDYLQRLACGPWAGEE
                     FVWVKPGDPISPTPFMTLHSISLP"
     gene            complement(4514..5149)
                     /locus_tag="AC16G0_02490"
     CDS             complement(4514..5149)
                     /locus_tag="AC16G0_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018204663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HIR5098519.1"
                     /translation="MDLNALKEAVIAGDLKGCGILTEVAIQAGIPPEKILNEGLVPGM
                     EVVGEKFRCNEVYVPEVLVAARAMKKSLSIIKPLLTKAGAKPVGVAVMGTVKGDLHDI
                     GKNLVCMMLEGGGFEVIDLGADVAPDKFVQAIKDTGAQLLGISSLLTTTMLGMREVIR
                     ALDQADLRAKVKVMVGGAPVTQTFADEIGADAYGESAAVAVEQAKSLLGIP"
     gene            complement(5402..5701)
                     /locus_tag="AC16G0_02495"
     CDS             complement(5402..5701)
                     /locus_tag="AC16G0_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098520.1"
                     /translation="MGILARLFGGKSTKDSDDAIHIYVECGRCKAKVHVRLDKRHDLS
                     QGEGGGYFVRKEIMDSKCFRLMTAEVTFDSAYGIHSQEVQGGRFLSKEEFEANVS"
     gene            complement(5946..>6186)
                     /locus_tag="AC16G0_02500"
     CDS             complement(5946..>6186)
                     /locus_tag="AC16G0_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098521.1"
                     /translation="AWQFGALDALTKQLAGAVTACVTMIRQLSLSQSDFVVFRQRVHQ
                     QLEPAVLALMNGELTREGVEQDLPWPHEGKRSGSA"
BASE COUNT         1115 a         1943 c         1946 g         1182 t
ORIGIN      
        1 gggagggggg aagagagagg gcgccagtcg aagctcaacc ctattccggc gaggaagacg
       61 atcaggccga gtgctcccgc gaggagtctg gatcgagacc tcccaaagcg tctcctgctt
      121 tccactgaag tgtctgatgt ccggtgtgga agcatccgcc ctcgccggga aaacgggtcc
      181 gagattgctg cctgccgagt gattcaccct ccgcgcttct gtcgtatctc gagtcacgac
      241 ccaaaattct cgggacttat agcacacgtc gaaaatgcga ggcaagaatt tttccccgac
      301 cgagaggatt tgtttcttcc gaaggccctg gggccgctca catcacgttg cgcaggtcgc
      361 cgcccagata gccggtgaca gcgatctgga gcgcaccgag cagggaaagg gtaaggagag
      421 ccccggcaat acgttcctgc gccctccgct tcggccttcc ctgatcatgt cttcttcctt
      481 tctgtccgtt tcattttgga atggtctatt cggctagaca tgcctcgtat tcaatcttga
      541 ccccacccag cccttcatgg gagacatgcc caggatcacc tctgtcagtc gtggctatcg
      601 gagttcgagt catggggccg gcgccccaaa gagtagccat aaggaacgcg cgggcctgcc
      661 tcgacccttt gccttccttc tcgtggctgg catcctatcg atcctcgagc atggacttct
      721 ttccacggga caacggaatg gttacgccca tcgtcaccgt cacgcccagc ccggcgctcg
      781 accggacgct ccgggtggac cgtctggact tcggggccgt cgccagggtc cgcgatgttc
      841 gcgaggaccc cggagggaag ggcgtcaacg tgtcccgagt gctcaaccag tttggctggc
      901 ctacggcagc cctgggcttc atcgggggga gcatcggcag gcgactggaa gaggagcttc
      961 accggtacgg gatcgtgacg gacttcgtcc ccgtcggggg cgaaacccgg atcaacctta
     1021 ccctcaccga tggggagagg gagctgaagg tcaacgaatc cgggcctccg gttgattcca
     1081 cggctgtagg ctccctgatc gagaaagtga gatgtcacgc ccggaggagc cgggctgtcg
     1141 tcctggcggg gagcctcccg cccggcgcgg gagagggtct gtacgcggaa ctggtggaag
     1201 tcatccggca ggaagggtct cgggccgttc tggacgctga ggggacgccg ctccgtctcg
     1261 gcgtcgccgc acgaccgttc ctggtcaagc ccaatctccg tgaggcggag gctctcctgg
     1321 agagaccgct ctccacggag gcggacatct gccaggccgc gcgacgtctg ggtgataggg
     1381 ggatcgaggt cgtggtcatc tccctgggcg gctcgggcgc catgatggct cgtcacggcc
     1441 gggtctggat ggcggagatt ccccagacca gaacggtgag cacggtgggg gctggggatt
     1501 ctttgctggc atacctgctc atggccatcc tgcaggatat ggatccagcc gcctccctcc
     1561 tcagggcgac ggcggcgggc ttggcctcca ccagtcttcc ggggagccag ctctgcacgc
     1621 cgccggaagt tgagcggatg atacccctgg tacgtacgcg agagctgtga gacaggggaa
     1681 gatgcgggat ccccacgtgg gatcgaaatg aggatgacgg gcacacctgg gattcagtcg
     1741 aggatgatgg cggcgctcct cacgggcgcg gagcgaattc aactggcgga agttccgcga
     1801 ccggtctgcc cggaagacgg actcctgctc cgggtggcgg catgtgccgt gtgcggatcg
     1861 gatgtccggt tatatctcgg gcggaagcgg atccagggcg ggcaggagat cgccggccag
     1921 ccgcttccgg gccacatcct cgggcatgag atcgcgggtg tgatccacga ggtcggctcc
     1981 tccgtaaggg gcttccgccc cggggaggct gtgatcgtcg cccccggcat cacctgtggg
     2041 aagtgtgatg cctgcctggg taggcgaccc gtggtctgta ggaactacca ggcgttaggt
     2101 taccagtttc cgggcggttt cgcggagtat ctggccgttc cagggtgcct actccaggac
     2161 ggcagcgtga acagggtccc tgaaggtgtc ccgtcgtgga aggcctgcct ggcggaaccg
     2221 ctggcctgca cgctcaatgc ccaggaggcc atgggcatca gagcggggga ttccgtgctg
     2281 gtcacgggtg caggacccat ggggtgcttg cacctgctgc tggcgcgtca gcgaggcgca
     2341 ggcttcatcg ccgtggccga acccgacgcc ggtcgccgag agcgtgctgg aagactgggc
     2401 gcggacctgg tcgtgaaccc ggacgcgccc aacctggagc agctactgct cggaggcaca
     2461 cagggcgagg ggttcaccgc tgtcatcttc gccgtctcaa gcatcgaggc tgttcgcctc
     2521 ctcttcactg cgtacgaggc cgggaggtat cgcctcgtgg cgcccggcgc acgcgtgaat
     2581 ttcttcgccg gcctcgatcc cggggagacc tccctgagcc ttgacgcgcg ggccattcac
     2641 taccagggcc tctgtcttct cgggtcggtg aactccacgc cgcgacagaa taccgaagcg
     2701 ctccgcttga tcgcgagcgg agcggtccct gtcgagtcat tggtcagcgc gcgcctcccc
     2761 ctttcccgag tgggagaagc gctcacgctg gccatgtccc gctcccacct gaaggttatc
     2821 gtcgagccag gagggagcga aggaaaagcc tagaatgatc cgcagattgc gcagattggc
     2881 cggcagggaa gatccgatcc cggaatctgc gagaatctgc gtaatctgcg gatagggtga
     2941 ggctttctct tcccttcaat ccctcacctg gaccaccatc tccacctcga ccgggatgcc
     3001 ctgcggcaac tggttggtgc cgatggccga gcgggcatgc ttgccgcgat ccccgaacag
     3061 cttgatgagc aattccgacg ccccgttcat gacccagggc tgacgcgtga agccgtccac
     3121 gctggccacg aagcccagaa gcttgaccac ccgctccacc ttgtccaggt cgccgatcag
     3181 gctcttcacg caggcgaggc agttgaccac gcagatctgc gcagcctggt acccctgttc
     3241 ctcggtcagc tcacgcccga gctttcccgt gaacttgggt tttccgtcca gcgtcggccc
     3301 ctgcccagca gtaaagatca agttgccgct ccgcacggcg ggtacgtagg ccgccaaggg
     3361 cttcggctcg ggcaggacga agcccatctt ctccagttcc tgttcgattc gcatggttcc
     3421 tcctgtgaaa ggaaaacagc tcaccttcgg gggcgccgag aggcagcacc aaattgactg
     3481 agcagcaggg ggaccttacc acagaggcgg catagaatcc aagcctaggt cgatgcgaga
     3541 gcagttcttt caagtgtcac cggtcccgga attttgcgat gggcagccgg gttgagagaa
     3601 gctcctcctc ccggaaggcg gccaactcca gatgtggcgc tacggcaggc tgatcgagtg
     3661 gagcgtcatg aagggggttg ggctgatggg atcgccgggc ttcacccaga cgaattcctc
     3721 gccggcccat ggcccgcagg cgaggcgctg gagatagtcg agggagccgg ggatctccgc
     3781 gtaggcgagg ttgaagagct cggccatctc ggccgcgtgg gcgcgaccag catcgatgtt
     3841 gccgaccccg tggtcgatga aggccacgcg cttgtagttt ttcagcagtt catgggcgat
     3901 gtatcgggcg tcctcttccc cgtacttggg cagatactcg ttcttgtagc agctgaggga
     3961 cgtggtcccg tagctcgccc agccgggcgt gatgtaatag gtcccggcac aggcggccac
     4021 ctcggcctcg taacgttcgc aggagcccag caagagcgcg atgcaatcgt ggaccttggg
     4081 catgagtatg ggggcggttc gagcggtgac gccgaccacc ccgttgctgc acaggccgta
     4141 tcccaggatc accaactcgg tgtcggccgg cagggcatcc acgacctcct gaatggcagc
     4201 cggcatcttc cccggcgttc ggtggaggcc ctgggggaga taggtgcgta tgaggtcggg
     4261 cggcgagacc gcctcgaact cgtccttgaa cacctcacag gcaatcaggt ggcgtttcgc
     4321 ctgcttctcc atgcccagcg cgtgttctcg ctctgtcaaa gccgccactc cagcctgcct
     4381 ccccaaatac gttcggcgaa tcaagagcgc aacaattcct tcggatccga tgaacacccg
     4441 aacagcccaa ctgcggcaca gacaatcgct ctacgctgct ccgtggtttc cgaattgtat
     4501 gggattcagc gtatcagggg atccctagga gcgacttggc ctgctccacc gccacggctg
     4561 cgctctcccc gtaggcatcg gcgccgatct cgtcggcgaa tgtctgggtg acgggtgcgc
     4621 cccccaccat gaccttgacc ttcgcgcgga ggtcggcctg atccagcgcc cggatgacct
     4681 cgcgcatgcc gagcatcgtg gtggtgagga ggctggagat cccgaggagc tgagccccgg
     4741 tgtccttgat agcctggacg aacttatccg gcgcaacgtc ggcaccgaga tcaatgactt
     4801 cgaaccctcc accctccagc atcatgcaga ccagattctt tccgatatcg tgcaggtccc
     4861 ctttgaccgt gcccatcacc gccacgccca caggcttggc cccggccttg gtaagcagtg
     4921 gcttgatgat ggagagagat ttcttcatgg cgcgggccgc caccagcact tcgggcacgt
     4981 acacctcatt gcagcggaac ttttccccca ccacctccat cccgggcacc aggccctcgt
     5041 tcagaatctt ctccggcggg atacccgctt ggatcgctac ctcggtcagg atgccgcatc
     5101 ccttgaggtc cccggcgatg actgcttcct tcagtgcgtt caggtccatg gcagctccct
     5161 tcgcggatga ccggcagata aaccctcctg aaatattgcg aaatatgccc caggaaaatc
     5221 gggaaggcca gcccgaaact gcatttgact gcttggacgg acgtgtccat gcaggggacg
     5281 gctgacggag cccggaggac gctgactcgg gactccggcc aggagagggc ccacacgagc
     5341 ccatgactcg acgtccatga gactgacggc cgaggcgcgg agggattccg ccctgcaacg
     5401 atcaggagac gtttgcctcg aactcctcct tcgagaggaa gcgaccgccc tgcacctctt
     5461 gcgagtgaat gccgtaggcg ctgtcgaacg tgacctcggc cgtcatcagg cggaaacact
     5521 tgctgtccat gatctccttg cgaacaaagt atccgcctcc ctcaccctgg ctgaggtcat
     5581 gccgcttgtc cagacggacg tgcacctttg ctttgcagcg gccgcattca acgtaaatat
     5641 gaatagcatc atccgagtcc ttggttgact tccctccaaa aagcctcgcg aggattccca
     5701 cgggctgccc tcctgtttgc ctgaccattg ccaggtggcg tcaaggctat ttgacagaat
     5761 atggcgtgta ccatatccca gccgctatct gtgggcaaga cccaggacgc gctgcggaca
     5821 tgttgtggat gggaaggatg ctggtggatt cccggctcac ctgacagaat ccgatcatct
     5881 cttgagtaac cgctgtggtt ccatctgccc atcgatcggc gcgatatgcg tagcggtccg
     5941 acgaattacg cgcttccaga ccgcttcccc tcgtgcggcc agggcagatc ctgctccact
     6001 ccttccctgg taagctcgcc attcatcagc gcaagcacgg cgggttcaag ttgctgatgc
     6061 acgcgttggc ggaacacgac gaagtcgctt tgggagaggc tcaactggcg aatcatcgtg
     6121 acgcaagccg tcacggcccc agccaattgc tttgtcagcg catcgagagc gccaaactgc
     6181 caggcc
//
LOCUS       DBNGZC010000131         5588 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_206168, whole genome shotgun sequence.
ACCESSION   DBNGZC010000131 DBNGZC010000000
VERSION     DBNGZC010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5588)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5588)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5588
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_206168"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1579)
                     /locus_tag="AC16G0_02505"
     CDS             complement(71..1579)
                     /locus_tag="AC16G0_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR5098522.1"
                     /translation="MGFSFDYLLQGFATALTLQNLFWAMVGCFIGTLVGVLPGIGPVS
                     GIAILLPLTNVLPPTSAIIMMAAIYYGAMYGGSTTAIVVNIPGEASSVPTAMDGYEMA
                     KQGRAGPALAISAIGSFVAGTLSVIALTFFAPMLAEFAVVFGPPEYFALMFMGLSLVI
                     SLSGRALLKGLISTAVGLGAALIGQDPLTGAARLTFGTVTLNAGVDFISVIVGLFAIS
                     EVMINVEQRITAISEFDINNWMPTWADIRQCLGTMVHSTGIGFFLGLLPGCAPSVVSF
                     VAYDVEKRVSKHPERFGKGAIQGVCAPETSNNATSTAGFVPLFSFGLPTAPSMAVMLG
                     GLMMYGLQPGPMLFAQSPQFVWAVIASMYIGNVMLLILNLPLVGMWAKICQIPFYILG
                     PIIVFCAVIGTYSIRFQAFDVWVMLIFGVLGYFMRKLGIPVAPMVLASVLAQMLETAL
                     GQALLISQGSVLIFFTRPISAVFMALAFLSIGRGFWVLMRAEAPEVAEEDPD"
     gene            complement(1589..2053)
                     /locus_tag="AC16G0_02510"
     CDS             complement(1589..2053)
                     /locus_tag="AC16G0_02510"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR5098523.1"
                     /translation="MKTQRVADMAVGCLVAAVGVFVLLASTWISVAGVHRLSPRTFPY
                     TVGVLLLACGVVLAVKSWTLREEGPTIAWPDRDGVRTITITILILACYIALMNVLGLP
                     LATFLYLAVATWYLKPSKWGVALLIGLITGGVSYYVFIQLLGLSFPAGFLFE"
     gene            complement(2437..3417)
                     /locus_tag="AC16G0_02515"
     CDS             complement(2437..3417)
                     /locus_tag="AC16G0_02515"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR5098524.1"
                     /translation="MLRMSGWLLVGVIVFSLIMPYAPGTAEAAFPERDITLLVPWPAG
                     GGTDTLARTLAKNAKKYFGVNMVVVNKVGGVGAIAMNSVATSKPDGYTLGVITDYLSL
                     YGLLGISELSFRDFELIALLNRSPAAISVRMDSQFKTLKDLLEYVKSNSGVLTVAHAG
                     PGQAWHLAVASLALKYNLKFVYVPFDGSAPVRTALVGGHVSVATTGIDEVLQFYGAKQ
                     LRILGVNSPARVSAFPDVPTFAEAGYPIENPIFAWRGLAVAKGTSPDVLKVLRDGFRK
                     AAEDPEYVKLMDDLALPRTYMEYDKFGEFLAATEKSLGPVLDSVGLLKKK"
     gene            complement(3791..4282)
                     /locus_tag="AC16G0_02520"
     CDS             complement(3791..4282)
                     /locus_tag="AC16G0_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098525.1"
                     /translation="MTAQTLDLAAVIARLEKVERQNWRMKVGGILILLVAGAGLLMGQ
                     AMPKVTTVEAEAFIVRDATGKQRAAFHLAPDGGVALSFFDPAGVGRAALRIDREGSPD
                     LTLIDQSAKNRAVLRVERNGAPGLTLFDQAGNPRATLHVVLDGSGLLQLSNKDEKLYT
                     RVP"
     gene            4890..>5588
                     /locus_tag="AC16G0_02525"
     CDS             4890..>5588
                     /locus_tag="AC16G0_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098526.1"
                     /translation="MSYRRYHIQSEPCPHVAPYPDRFQVRVIRWRLARLLLRELLHYR
                     FNLPVVTSRPCVYGTFSGPVGGFAPRPGQCVGCLRCTLEYPDMVRVRRNPARLRLGDS
                     YFTPEKVDTVLQEAATGQIPVRGAGYRGAFGGEGWDGMWTDMSEIVRPTRDGIHGREF
                     ISTDVDLGERPGVLDLDETGVPSAPLPGSLHLPIPLLFDAQPVLVEEPGLCRVLAEAA
                     GRLQILAVLPLRRLL"
BASE COUNT         1073 a         1828 c         1717 g          970 t
ORIGIN      
        1 gagatcccgt actcctctcg tgctccgcta accgcggaaa gctgattgct gatagctcca
       61 gccctacacc tcaatccgga tcctcctcgg cgacttccgg cgcctcggcg cgcatcagta
      121 cccagaaccc tcgcccgatg gagaggaagg cgagcgccat gaagacggcc gaaatgggtc
      181 gggtgaagaa gatcagcacc gacccctgcg agatgagcag ggcctggccc agggcggtct
      241 ccagcatctg ggccaggacg ctcgccagga ccatgggggc gacggggatg ccgagcttcc
      301 gcatgaagta gccgagcacc ccgaagatga gcatgaccca gacgtcgaag gcctggaacc
      361 gaatgctgta ggtgccaatc acggcgcaga agacgatgat cggccccagg atatagaagg
      421 ggatctggca gatcttggcc cacatcccga ccagcggcag gttaaggatc aggagcatca
      481 cgttgccgat gtacatgctg gcgatgaccg cccacacgaa ctgagggctc tgggcgaaga
      541 gcatcggccc gggctgaagc ccgtacatca tgaggccacc gagcatgacg gccatcgagg
      601 gggccgtggg caatccgaac gagaagagcg gcacgaagcc ggcagtcgac gtggcgttgt
      661 tcgacgtttc tggtgcgcag accccctgta tggccccttt cccgaaccgc tcgggatgtt
      721 tcgagacccg cttttcgacg tcgtaagcca caaacgacac gaccgacggg gcgcagccgg
      781 ggagcaatcc gaggaagaag ccgattccag tcgagtggac catggtcccc aggcactgcc
      841 tgatatcggc ccaggtcggc atccagttgt tgatgtcgaa ttcactgatg gccgtgatcc
      901 gctgctcgac gttgatcatc acctcgctga tcgcgaagag tcccacgatc acgctgatga
      961 aatccacccc ggcattcagc gtcacagtgc cgaaggtcag ccgggcggcg ccggtcagcg
     1021 gatcctgtcc gatcagggcc gcgccgaggc caacggccgt ggagatcaac cccttcagga
     1081 gggcgcgccc ggagaggctg atgaccaggc tcaggcccat gaacatcagc gcaaaatact
     1141 ccggcgggcc gaagaccacg gcgaactcgg ccagcatcgg cgcgaagaag gtcagggcga
     1201 ttacgctgag cgtgcccgcg acgaaggagc cgatggcgga aatggccaag gccgggccag
     1261 cccgcccctg cttggccatc tcgtagccgt ccatcgcggt cggcacggag gaggcctcgc
     1321 cggggatgtt caccacgatg gccgtcgtcg agcccccgta catggccccg taatagatcg
     1381 ccgccatcat gatgatcgcc gaggttgggg ggagcacgtt ggtcaggggc agcaggatgg
     1441 cgatcccgga gacgggcccg atgccgggga gcacgccgac cagggtgccg atgaagcagc
     1501 cgaccatcgc ccagaagaga ttctggagcg tgagggcggt cgcaaaccct tggaggagat
     1561 agtcgaagga gaagcccatg ccgtccccct attcgaacag gaatcccgcc gggaacgaga
     1621 ggcccaacag ttggatgaac acgtagtacg acacgccgcc ggtaatcagg cctatgagca
     1681 gggcgacccc ccatttcgac ggcttcaggt accaggtggc gacggcgagg tacaggaagg
     1741 tcgccagagg cagcccgagc acattcatta gggcgatgta gcatgccaga atgaggatgg
     1801 tgatcgtgat cgtccgcacg ccatccctgt ccggccacgc gatggtgggc ccttcctccc
     1861 ggagcgtcca ggacttaacg gccagcacga ccccgcaggc gagcaggagt acgcccacgg
     1921 tgtaggggaa ggtgcgcggg gagagtcggt ggacacccgc cacagagatc cacgtcgagg
     1981 ccaacaacac aaaaacgccg acggctgcaa cgaggcatcc cacggccata tcggcgacgc
     2041 gttgtgtctt cacatccacc tccgaatgtc gcgcgccctt tgaggcaata ccgcccgtcg
     2101 ggcgaaaagt aatgagccct tccccgcttc ctggtcaaga agcgctgcgt acaacgccgg
     2161 cggaaccccg cgagatgggc gcggggcctg atccaccgat ggcgcccggc ctgtccccac
     2221 accggtgcta ccaccggcag gtctccgggc gaaatcacga ccgcctgggc cagcagtgtc
     2281 ttatcatgag ccaagcagct cgtcatagag ataaccgtga tgtgtacaat gtgccagaca
     2341 acgccccggc tgcttgagtc catggtagac gccacacaaa aaccacgagc gtcggggcga
     2401 ccccgacgct cgtggagact gtcaatctcg gattgcttat ttctttttca ggagtccaac
     2461 ggagtccaac accgggccca ggctcttctc tgtcgcagcc agaaactccc cgaacttgtc
     2521 gtactccatg tatgtccgcg gcaaggcgag gtcgtccatg agcttcacgt actccggatc
     2581 ctctgccgcc ttccggaatc catcgcggag gactttgagc acatcgggag acgtgccttt
     2641 ggccaccgcc agccccctcc aggcgaagat cggattctcg atgggatagc ccgcctccgc
     2701 gaaggtgggg acgtccggaa aagcggagac acgagctggg ctgttgacgc ctagtatccg
     2761 gagctgcttc gccccataga actgcaacac ttcgtctatc cccgtcgttg ctacgctgac
     2821 gtgaccaccg acaagcgcag tgcgcaccgg cgcagatcca tcaaaaggga catagacaaa
     2881 ctttagatta tacttcaggg cgagcgaagc cacggcgaga tgccaggctt gccccgggcc
     2941 ggcatgggct accgtcaaga cgccggaatt gctcttgacg tactccagca ggtctttcaa
     3001 agtcttgaat tgcgagtcca tccgcactga gatggcggct gggcttctgt tcagcagcgc
     3061 gattagctcg aaatcccgga aggacagctc ggagataccc agaagaccat acaggctcaa
     3121 gtaatcggtg atgaccccga gggtgtaccc atccggcttg gatgtcgcca ccgagttcat
     3181 cgcgatagcc ccgacgccac ccaccttgtt caccacaacc atgttaaccc caaagtactt
     3241 ctttgcgttc tttgccagcg tccgggccaa cgtgtccgtc ccaccgcccg ccggccaggg
     3301 gaccagcaag gtgatgtccc gctccgggaa ggccgcttcg gccgtcccgg gtgcataggg
     3361 cattatcagg gagaagacta tgaccccaac cagaagccaa ccagacattc ggagcatgaa
     3421 tgcctccttt catgaatgga tggtgaggtg tagacattcg aacctgggct taaaccgcct
     3481 tgacccagga ggagcatggc ctaagcgcca ggtgctgtca agacaggtcc aaacccatct
     3541 gggggcggcc gcgagcagta gcgtactacc taccatgccc caataagcgc acctcttccg
     3601 cggaggccgg gggtgggtgg gcagaagctc gacttctcag cagttttgcc ggtgatcgta
     3661 aattgtctca gacccccctt tacggggggt ctgcccgcat cgtcctgccc tcctgcctca
     3721 tcatctgcga gggtgcccca cacaggttat cgcagtccct gccgagcgat gccgccttgg
     3781 tagggccctg ttacggcacc ctcgtgtaca acttctcgtc cttgttcgaa agctggagga
     3841 gccccgaccc atccaacacc acatgcaacg tggcgcgcgg atttcccgcc tggtcgaata
     3901 gtgtcaggcc cggcgcccca ttccgttcca ctcgcagcac ggcgcggttc ttggcggact
     3961 gatcgataag ggtcaagtcg ggcgacccct ccctatcgat ccgtaatgcg gcgcggccta
     4021 caccggccgg gtcgaagaag ctcagcgcca cccctccatc gggtgccaga tggaaggcgg
     4081 cgcgttgttt tcccgtcgca tcccgcacga tgaacgcctc cgcttccacc gtcgtcacct
     4141 tcggcatcgc ctgacccatc aacaggccgg cgccggcgac cagcaagatc agaatcccac
     4201 cgaccttcat gcgccagttc tgtcgctcaa ccttctccag tcgagctatc actgccgcca
     4261 gatccagtgt ctgtgctgtc attgttgttc cctcctccgc acttggcttc tccacctgta
     4321 cgtacgtgtc aaacatacgg gatgtggtca aggatgacaa gttgcctttc cgggagccgg
     4381 cctttctccg cgtgcgctgc ctggctccgc accctgacgt gggggggcgt gtccgaggca
     4441 accaagcggc cctgacgggg catgctgaaa ggcccgctag cccaacttgt ttttctcggc
     4501 agtgtcgcgt gttgttgtcc gttggatgtg gattcagacg cggggaccag gacgggacct
     4561 gtgacactgt ccgggtcaga gcatcgcaag cccgccgact gctggggatc ggcctgggct
     4621 cgttccttcg ccgcatttgg atgaaagacc gaacgactgg acccgcggcg gctgattcct
     4681 gggcttggag gggaggaagc ttcgccgcct ttgacgaccg cgcccgaggg tggtatgttc
     4741 cgggcgtccg gccaccccgg atgcatgcgc gggcctacca tccgtgctgc ccttcccacc
     4801 tggggggtgg gttccggcgg gaaggcggtg ggagccgcct ctcgcgcgac gcgtcacttt
     4861 ggagtgtcgc cggaggcgac cgctctgctg tgagctaccg ccggtaccac atccaatccg
     4921 agccctgccc gcacgtggcc ccataccctg atcgattcca ggtcagggtg atccgctggc
     4981 gcctcgcccg gctgctcctt cgcgagctcc tccactaccg gttcaatctg cccgtcgtca
     5041 ccagccgacc ttgtgtctat ggcacgttct ccggcccagt cggcggcttt gccccgcgcc
     5101 ccggacaatg cgtgggctgc ctccgctgca cactggagta cccggacatg gtccgggtcc
     5161 gcaggaaccc cgcccggctc cgcctaggcg actcctactt cactcccgag aaggtggaca
     5221 cggtcctgca ggaggccgcg accggacaga tcccggttcg aggtgccggc taccgtggcg
     5281 ccttcggtgg agagggctgg gacggcatgt ggacggacat gtccgagatc gtccggccca
     5341 cgcgcgacgg catccacggg cgcgaattca tctcgacgga cgtggacctg ggggagcggc
     5401 ccggggtcct cgacctcgat gagaccggcg tcccttccgc cccgctgccg ggatcgctcc
     5461 acctgccgat ccccctgctc tttgacgccc aacccgtcct ggtcgaggag cccggcctct
     5521 gccgagtcct cgcagaggct gcgggccggt tgcagattct ggccgtcctg ccgctccgta
     5581 ggcttctg
//
LOCUS       DBNGZC010000132         2221 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2065065, whole genome shotgun sequence.
ACCESSION   DBNGZC010000132 DBNGZC010000000
VERSION     DBNGZC010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2065065"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..1157
                     /locus_tag="AC16G0_02530"
     CDS             201..1157
                     /locus_tag="AC16G0_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR5098527.1"
                     /translation="MATLTCVVVPPLRPAGAAEAANPYFPKPVALQAKVQFWKQIYSE
                     YGVADFVLHDRDNLAAIYAVVRVEGASNESRAATAAQPMIQQLRERYAAVLEALAQGA
                     PPESLGADGLRLAQFWGCPCAPEVLHRAAANIRVQQGHRERFQAGLQRAQALLPPMLS
                     ILRRYNLPEELAALPMVESSFNPGAQSKAGAVGLWQFIQSTGKQYLTITRWRDDRSDP
                     LLATEAAARLLRDNYEALGSWPLAITAYNHGTGGVLAARAAVGSDSIDTIIDRYTGPR
                     FGFASRNFYAEFLAALELIQPTILEHSLSSDSRPPSRPTRQG"
     gene            1277..1576
                     /locus_tag="AC16G0_02535"
     CDS             1277..1576
                     /locus_tag="AC16G0_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098528.1"
                     /translation="MAHTCIIQGESGMPDDGYRTVIEITAHIPIPDSNAVRGLSPWIT
                     TILPCPDCGQTMIWAEDGYGPWHRICNGCGSHWSVTPEGEVEGRNRIVIERAKFS"
     gene            complement(1611..>2221)
                     /locus_tag="AC16G0_02540"
     CDS             complement(1611..>2221)
                     /locus_tag="AC16G0_02540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5098529.1"
                     /translation="LRALLDYARPRRPNLRAQALVPILGRVADLLLDRSRRRRVRVEL
                     DVSAGLPHVLADPDQLQQLFLNLLSNALDASPPGETVRVAVGPDPMLPAEDRAGIVRG
                     KADGPTLAIHIFNSGKGLTADQLEHVFEPFFSTKAQGQGTGLGLPIVEQIVRAHQGEI
                     EMLSVPERGTEVIVRLPFASKAEGDPVPELEECMAGAGQHGR"
BASE COUNT          391 a          784 c          666 g          380 t
ORIGIN      
        1 tggaggttga gggtggttca gcaggcgaga gaaggttccc gatgattcga ggtacttgcg
       61 gctcaacggg acacacggac gaggccgatc aggccgcagg tacgtgcggc tcgatctgcc
      121 acgacctgct tccgcctgaa ccaagaatat atcggcaggc tgaaagagga ttccacctcc
      181 gttggttccg cctcctcctg gtggccacgc tgacatgtgt cgtcgtgccc ccactgcgac
      241 ctgctggtgc cgctgaggcc gccaacccct acttccccaa acctgtcgcc cttcaggcca
      301 aggtccaatt ctggaagcag atctactccg agtacggagt ggccgacttc gtcctgcatg
      361 atcgggacaa cctggccgcg atctatgccg tggtgcgcgt cgagggggcc agcaatgaat
      421 cccgcgccgc tacggcggcc cagcccatga tccagcaact gcgggagcga tacgcagccg
      481 ttctcgaggc tctggcccag ggcgccccgc ccgaaagcct gggagccgac ggactccgtc
      541 tggcccagtt ttggggctgc ccgtgcgcac cggaggtcct ccaccgcgcc gccgccaaca
      601 tccgggtcca gcagggccat cgcgagcgat ttcaggcggg cctccagcgc gcccaggccc
      661 ttctgcctcc catgctgtcc atcctcaggc gctacaacct gcccgaggaa ctggcggctc
      721 tgcccatggt ggaatccagc ttcaacccag gcgcccagtc caaggccggg gctgtgggac
      781 tctggcagtt catccaatcc accgggaagc agtatctcac catcacgcgg tggcgggacg
      841 accgtagcga tccgctcttg gccaccgagg ccgccgcccg cctgctcagg gacaactatg
      901 aagccctcgg ctcttggccg ttggccatca ccgcgtacaa tcacggcaca ggcggcgtcc
      961 tggcggcccg ggcagcggtc ggaagcgatt ccattgatac aatcatcgac cgctacaccg
     1021 gcccccgctt cggcttcgcc tcgcgaaact tctacgccga gttcctggcc gccctcgagc
     1081 taatccagcc caccattctg gagcacagcc tgtcctcaga tagtcgcccg ccatcaagac
     1141 ccacccgaca ggggtaggcg accgtgggct tccgaggggt ctcgtccctc tgccgcggca
     1201 ccgcatgaca ccgcccgcgt gactgtgata cacttccgtc taacctcgcg tctcgacttc
     1261 acccctggag cgccgcatgg cccacacctg catcattcaa ggggaaagcg gcatgccgga
     1321 cgacggttac cgcacggtca tcgagatcac cgcgcacatc ccgatccctg acagcaacgc
     1381 cgtccgcggc ctttcgccct ggatcaccac gatattgccg tgtcctgact gtggacagac
     1441 catgatctgg gccgaggacg gctacggccc ctggcaccgc atctgtaacg gctgcggttc
     1501 tcactggtcc gtcactcccg agggagaggt cgagggccgc aaccgcatcg tcatcgagcg
     1561 tgccaagttc tcttgacggc gggaaagacc tcggcacgcc tgccggcacc tcagcgtcca
     1621 tgctggcctg ctcctgccat gcactcctcc agttccggga ccggatcccc ctctgccttc
     1681 gaggcaaagg gcaggcggac gatgacctcg gtcccacgct ccgggacact tagcatctcg
     1741 atctcgccct ggtgtgcgcg gacgatctgc tccacgatgg gcagtccgag cccggttccc
     1801 tgtccctgcg ccttggtgga gaagaagggc tcgaacacgt gttccagttg gtcggcagtc
     1861 agtcccttgc cggaattgaa aatgtgaatc gccagcgtgg gtccatccgc cttgccccgc
     1921 acgatcccgg cccgatcttc ggccggcaac atgggatccg gtcccacggc cacccgcacc
     1981 gtctcacccg gcggcgaggc atccagtgca ttcgacaaga ggttgagaaa gagctgctgg
     2041 agctgatccg gatctgccaa gacgtgagga agacccgctg acacgtccaa ttcgacgcgg
     2101 accctgcgcc ggcgactccg gtcgaggagg agatctgcca cccgcccgag gataggcacc
     2161 agtgcctgcg cacgcaggtt gggtcgtcgg ggccgggcgt aatccagcag ggcccgaagg
     2221 g
//
LOCUS       DBNGZC010000133         4622 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1283093, whole genome shotgun sequence.
ACCESSION   DBNGZC010000133 DBNGZC010000000
VERSION     DBNGZC010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1283093"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="AC16G0_02545"
     CDS             <1..320
                     /locus_tag="AC16G0_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013362.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIR5098530.1"
                     /translation="RPASKWRRQADDLGRAATLQAGLLAGVAVAVARDGLLVYSTCSL
                     EPEETETIVTAFLALHPEFHLEAPGAIWPEADLSNTAGLLRTWPHRHGTDGFFVARLR
                     RDA"
     gene            362..1066
                     /locus_tag="AC16G0_02550"
     CDS             362..1066
                     /locus_tag="AC16G0_02550"
                     /inference="COORDINATES: protein motif:HMM:NF015734.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIR5098531.1"
                     /translation="MHGLLKFLGTVTGLIALALLSAFVAMHLVMEKDRVEVPRTVGLD
                     SVAATQLIEEVGLAPRVLAEEFSPTIPKGRVARQRPPVGARVKLGSEVRLILSRGTDQ
                     LEVPDLAGESLPQAQRILAEAGLSLGGITRIHSDVHPQETIIAQDPPAGSTATRGAMV
                     TMLQSLGPWDEAITMPDLLGREFVTAMNLLKELQLGVRVTFEKAATREGHVVAQVPLP
                     GEKTRVGKQVQITVGE"
     gene            1139..1789
                     /gene="rpe"
                     /locus_tag="AC16G0_02555"
     CDS             1139..1789
                     /gene="rpe"
                     /locus_tag="AC16G0_02555"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003394996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR5098532.1"
                     /translation="MIKIAPSVLSADFAILGEEIAKVEPVADLLHLDVMDGHFVPNLT
                     IGPMVVEAIRRRTRVPLDVHLMIQNPERYIGAFAQAGATFLTVHAEVCPHLHRTIQQI
                     REAGIKAGVALNPSTSLHAVENILDQVDLVLIMSVNPGFGGQSFIPFVHKKIEELARR
                     LHNATPRPELEVDGGIKLENCHEVAKAGATILVAGSAIFHAPDPRAAVLKMKEQVA"
     gene            1975..3012
                     /locus_tag="AC16G0_02560"
     CDS             1975..3012
                     /locus_tag="AC16G0_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012627648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIR5098533.1"
                     /translation="MSTRQLPIPPHFDPGAVGRVWRVPYQQRAVEAEAWAKLHDVPAA
                     AADRRRACLVIVDCQNTFCLPEFELFVGGRSGMGAVEDNVRLCEFIYRNLGLLTEIVP
                     TMDTHTAIQIFHPVFWIDAAGKHPNPHTVISLEEVETGAWKPNPAVAASVMDGDARRL
                     QRHALYYVKRLTDRGKYPLMVWPYHSMLGGIGHALVSAVEEALFFHCVARSSQTHFQI
                     KGGNPLTEHYSVLHPEIQEGPDGEPIAERDTGFLQRLLEFDLVIVAGQAKSHCVAWTI
                     DDLLTEVRLRDPKLVEKVYLLEDCTSPVVVPGVVDFTEAATAAFQRFAAAGMHVVRST
                     DPMHAWSGVDR"
     gene            3101..3697
                     /locus_tag="AC16G0_02565"
     CDS             3101..3697
                     /locus_tag="AC16G0_02565"
                     /inference="COORDINATES: protein motif:HMM:NF025173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0044183 - protein folding chaperone
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HIR5098534.1"
                     /translation="MGRKGLMGLAVTLAIALAFPASAQMYGPMGPGGPMGGERMGPGF
                     REEREGRMMSHEGPLITIMLEHSQELGLTLEQQQKLRDLRAQFSKESVRRTAEIRVAE
                     IELDTLLEQDQWDLSKIEPKVKQIAALQGDLRLARVKTLAAGRAVLTPQQLENLKQVG
                     HRMRLQGGPGSPGPGMMGPGPYGGPGMRGPGGPPTPRQ"
     gene            3940..>4622
                     /locus_tag="AC16G0_02570"
     CDS             3940..>4622
                     /locus_tag="AC16G0_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013324.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HIR5098535.1"
                     /translation="MRLIFGTILVLAGIAISGVLTRLLARPTREQGLAGVEGGLKALR
                     FIAMVLVPIGALMILSSAIRMIGPGQVGVALLFGKVQAVALGEGINLVNPLYDVVQMD
                     TRVQKHQARYDAASKDLQAVHVEMVLNYRLLPDKATEVYQRIGPNYAATIIDPAAQEV
                     LKANTALHIATEILHQRPKIKADVQKDLTAWLAKYGIEMKEAALANIRFDAAYEKAIE
                     AKQIEEQKA"
BASE COUNT          831 a         1406 c         1608 g          777 t
ORIGIN      
        1 gccggccggc gagcaaatgg cgccgtcagg cggatgatct cggccgcgcg gcaacgcttc
       61 aggccggatt gctggccggc gtggcggtgg cagtggccag ggatggcctg ctggtgtaca
      121 gcacgtgcag cctggagccg gaggaaacgg agaccatcgt cacagccttt ctggccctcc
      181 acccggagtt ccacctggag gctcctggag cgatctggcc ggaggccgat ctctcgaaca
      241 ccgccggact tctccggact tggccgcatc gccacgggac cgacggtttc tttgtcgcgc
      301 gcctgcgccg ggacgcatga tgccacctcg tcagaatcag ccctcgcggc tccgcactct
      361 gttgcacggc ctgctcaagt ttctgggcac ggtcacgggt ctgatcgcct tggccctgct
      421 ttcggccttc gtggccatgc acttggtcat ggagaaggac cgggtcgagg ttccgcgcac
      481 cgtgggtctg gactcggtcg ccgccacgca gttgatcgag gaggtcggcc tcgcgccgcg
      541 cgtcctggcc gaagagttca gccccacgat ccccaagggt cgcgtcgccc gtcagcggcc
      601 gcccgtgggc gcaagagtca aactggggag cgaggtgcgg ctcatcctca gccgggggac
      661 cgaccagttg gaggtgcccg atctggcggg agagagtctc ccccaggcac agcgcatcct
      721 ggcggaggcg ggcctcagcc tgggaggcat cacccgcatc cactcggatg tccacccgca
      781 agagacgatc atcgcccagg atcccccggc aggttcgacc gccacgcggg gggccatggt
      841 gactatgctg cagagtttgg gtccgtggga tgaggcaatc accatgcccg acctgctcgg
      901 ccgggaattc gtcacggcca tgaaccttct gaaggagctg caactggggg tgcgggtgac
      961 cttcgagaag gcggccacgc gcgagggtca tgtggtcgca caggtccccc tgccggggga
     1021 gaagacgaga gttggcaagc aggttcagat taccgtggga gagtaggtcc gcggcccgtg
     1081 tgccgcaggg ttgcccattg caggcaagaa cgcacgaccg ctccctggag gaggccccat
     1141 gatcaagatc gctccctcgg tgttgtcggc agacttcgcg atcctgggcg aggagatcgc
     1201 caaggtggag cccgtggcgg atctgctgca cctggacgtc atggacggcc actttgttcc
     1261 caacctcacc atcggcccca tggtggtgga ggcgatccgt cgtcggacgc gggtaccgtt
     1321 ggatgtgcac ctgatgatcc agaatccgga acgctacatc ggggcgttcg cccaggccgg
     1381 agccaccttt ctcaccgtgc atgccgaggt ctgcccgcac ctgcaccgga ccatccagca
     1441 gatccgggag gccgggatca aggccggcgt ggcgctgaac ccctccacct cgctgcacgc
     1501 ggtggagaac atcctggacc aggtggacct ggtgctgatc atgtcggtga atccaggatt
     1561 cggcgggcag tccttcatcc cttttgtcca caagaagatc gaagagctgg cgcgccggct
     1621 gcacaacgcc acaccacgac cggagctgga ggtggacggg gggatcaagc tggagaactg
     1681 ccacgaggtg gccaaggcgg gcgcgacgat cctggtggcc gggtcggcca tcttccacgc
     1741 gccggacccg cgggccgcgg tgctgaagat gaaggagcag gtggcgtagg aggggtcagc
     1801 gctcggcact tcggtggttg ggctgcacgg cggtacacat cgactgcgtg ttccctggat
     1861 ggccgaacgc ccgaactgcc gacctgctga acggccgagc gcgtgcgctc gaggaggccg
     1921 aggaatgtgc tggttggctg gcgaggtcga caggaaccaa gagggtggcc gcccgtgagt
     1981 acacggcaac ttcccattcc accgcacttc gatcccggtg cggtcgggag agtctggcgg
     2041 gttccttacc agcagcgggc cgtggaggcg gaggcctggg ccaagctgca cgacgtgccg
     2101 gctgccgcag ccgacaggag gcgtgcatgc ctggtgatcg tcgattgcca gaatacattc
     2161 tgtcttcctg agttcgagct cttcgtcggc gggcggtctg gcatgggcgc ggtggaggac
     2221 aacgtccggc tgtgcgagtt catctaccgg aacctgggtc tcctcaccga gattgttccg
     2281 accatggaca cgcacaccgc catccagatc ttccatcccg tgttctggat cgacgcggcc
     2341 gggaagcacc cgaaccccca cacggtcatc tctctggagg aggtggagac gggagcctgg
     2401 aaacccaacc cggccgtcgc cgccagcgtg atggacgggg atgcccggcg gctgcagcgc
     2461 catgcgttat actatgtgaa gcgcctgacc gaccggggaa agtacccgct catggtctgg
     2521 ccctaccact ccatgctggg tgggatcggc cacgcgctcg tatcggcagt cgaggaggcg
     2581 ctgttcttcc actgtgtggc acggagcagc cagacccact tccagataaa gggcgggaac
     2641 ccgctgaccg agcactattc ggtcttgcac ccggagatcc aggagggccc cgacggcgag
     2701 ccgatcgccg agagagacac cgggtttctc cagcgtctgc tggaattcga cctggtgatc
     2761 gtcgccggcc aggccaagag tcactgcgtg gcctggacca tcgacgatct tctcaccgag
     2821 gtcaggctgc gggatccgaa gctggtcgag aaagtctacc tgctggagga ttgcacgtca
     2881 ccagtcgtcg tgcccggtgt ggtggacttc accgaggcgg cgaccgcggc gtttcagcgg
     2941 ttcgctgccg cggggatgca tgtggtgcgg tcgaccgacc ccatgcatgc ctggtctgga
     3001 gtcgatcgct gagtgaggta ccatggtcgc atacggcaag ggttccagga cagataatgc
     3061 aggagtgaat ccacgaagct tccaggaagg aggactggcc atgggaagga aagggctgat
     3121 ggggctggcg gttaccctgg ccatcgccct ggcgtttccg gcatccgccc agatgtatgg
     3181 tccaatgggg ccggggggcc cgatgggtgg tgaaaggatg ggtcccggct tccgcgagga
     3241 acgggaaggc cggatgatgt cccacgaggg tccgctcatc acgatcatgc tggagcacag
     3301 ccaggaactc gggctgaccc tggagcagca gcaaaagctc cgcgacctgc gcgcgcagtt
     3361 ctcgaaagaa tccgtgcggc gcacggcgga gatccgcgtg gccgagatcg agctggacac
     3421 cctgctggag caggaccagt gggacctgtc gaagatcgag ccgaaggtca agcagatcgc
     3481 tgcgcttcag ggtgatctcc gtctggcgag ggtcaagact ctggcagcgg ggcgcgcggt
     3541 cctgaccccg caacagctcg aaaatctcaa gcaagtcggc catcgaatgc ggttgcaggg
     3601 aggaccgggc agccctggcc caggcatgat gggcccggga ccgtacggcg gtccggggat
     3661 gcggggtcct ggcggcccac ccaccccgag gcagtagcgg cggcggcagt gcttaggccg
     3721 aggagcagag gaaagggcac tgggcacagc gcaaagagca cagggcacgg agcacagcgc
     3781 acagagggac gggaggttcg aagttagggg ggatcaacct agttcttcct cccgtgccgc
     3841 ctgctgcctt gtattgcagg tttccgtctt tggtcattgg tcattgtttc attccggtgt
     3901 cattggtcat tccgcagggg aggggcaggg ggcatcatca tgcgactgat ctttggaacg
     3961 attctggtgc tggcagggat cgcgatctcg ggagtactca cgcgtctcct cgcccggccg
     4021 acacgggagc aggggctcgc gggggtcgag ggtggtctca aggccctgcg cttcattgcc
     4081 atggtcttgg tgccgatcgg ggcgctcatg atcctctctt cggcgatacg gatgatcggt
     4141 cctgggcagg tgggagtggc gctgctcttc ggcaaggtcc aggcggtcgc cctgggcgag
     4201 ggcatcaacc tcgtcaatcc gctgtacgac gtggtgcaga tggatacccg ggtccagaag
     4261 caccaggccc gctacgacgc ggccagcaag gacctccagg cggtgcatgt ggagatggtg
     4321 ttgaactatc ggctcctgcc cgacaaggcg acggaggtgt accagcgcat cgggccgaat
     4381 tacgctgcca ccatcatcga tccggcggcg caggaagtcc tcaaggccaa caccgcgctc
     4441 cacatcgcca cggaaatcct ccaccagcgg cccaagatca aggcggacgt gcagaaggat
     4501 ctcaccgcct ggctggcgaa gtacgggatt gagatgaagg aggcggccct ggcaaacatt
     4561 cgcttcgacg ccgcctacga gaaggccatc gaggccaagc agatcgagga gcagaaggcg
     4621 ga
//
LOCUS       DBNGZC010000134         4749 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1967267, whole genome shotgun sequence.
ACCESSION   DBNGZC010000134 DBNGZC010000000
VERSION     DBNGZC010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1967267"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="AC16G0_02575"
     CDS             complement(<1..1266)
                     /locus_tag="AC16G0_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013986.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HIR5098536.1"
                     /translation="MAISVGIDIGAISVKAAVLGTAGDRPMLESLALTPDFAWLSFPD
                     PILVSSPCRALGDPLGVAEALLANVLRLIPGLPPGHVGATGCGAGLLQARRGIPRVND
                     AKAIAMAMGLLHPEVATVFEMGGQSSRFLQLKSNGSTRQARLQDYETNGDCAAGTGAF
                     MDQQATRLRYQIEEIGRIACAAPSAARIAGRCSVFAKSDMIHAQQKGADPPQILRGLC
                     DAVARNFKGSITKGKPAVPPVAFIGGLALNAGVIRALRELFHFDERQLFVPEAYAWFG
                     AIGAALLGAVGNGRRTGAWPSRRPSATPTEGNGDGATSRPLNLEQVLLLRDRVQPYRL
                     PEDRSVQDAYLGIDVGSVSTNLVVLSDAAEVMHEIYVWTEGRPTQVVSQGLRTIVTAL
                     GDRIRIRGVGTTGSGRELIGELVGADTLTD"
     gene            complement(1402..1710)
                     /locus_tag="AC16G0_02580"
     CDS             complement(1402..1710)
                     /locus_tag="AC16G0_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098537.1"
                     /translation="MSQEAFEPFEVVCPCCQAKLRVDPALRAVLSHELPPEERSVKDL
                     TEAVKGLKAEAAHRQEKFVESMKAEKGKKDLLDKKFQDALKRTKDVPITKPIRDIDLD
                     "
     gene            1876..2796
                     /locus_tag="AC16G0_02585"
     CDS             1876..2796
                     /locus_tag="AC16G0_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5098538.1"
                     /translation="MNPARRALDGRAMIIVVTLCFLWGLSQVSIKVANGGVSPVFQAA
                     VRSVVASILVALWSRARGLPLIHRDRTLFHGLAVGLLFGTEFVCIYLGLNYTTASHAV
                     IFLYAAPFFVALGAHWLLPHEPLTPRKLAGLTLAFLGVAVTLRESLGTPTSLQVVGDL
                     LSLTGGFLWAATSLYLKRVVHTSMTPSQMLFYQLGVSALQLSLMSAIFEPHPIRELSP
                     LVLGCLAYQALIVAFASYLVWFWLIQVYPVSNLSGFTFFTPIFGVLLGGLLLHEPLTP
                     SLLAGAALVAGGMILVNWPEKIGGKRGERT"
     gene            3182..>4749
                     /locus_tag="AC16G0_02590"
     CDS             3182..>4749
                     /locus_tag="AC16G0_02590"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HIR5098539.1"
                     /translation="MQATTEKVAPAFRGSISRRFTILFVHFLAFVAVVGALSVYKANR
                     IARLHTEIGELGAHIEVLDRLETAIFRLRAESQDSLFLGEAFHDPWLDEIPRQIRKQW
                     RHFEQLHSHDGHPWETGPSLEHALYQELDPRLDTFIAIARGAAKGTRPEGSKLREAAF
                     VLDAEAHALEDGLERLSALHRGLMADRSRAAGADADFILWANASFIAFLGLTVVVASS
                     LFVRRVALPLRRLNIAALDVADGHFPLQVPVKSRDEIGRLADTFNHMSEALSERDAQL
                     RRRAQEMEALSRIGTDISSMLNLDRLLQTIVDSARTLLRTDVAALCLLRDGGPALDVV
                     ATAGPWRPVMGSAPAVSTDDLGTDCDEICVRCQEARSTAGMAQLSARLKLGDKPLGAL
                     CVGSTGHRAFGEVERNVLSGLAAQAAIAIGNARLYERARGMAAVEERERLAREMHDGL
                     AQALSFLNFKLALTDRLLDGGPVAPVHQEVAEMQKVTAGAYAELRQAIFGLRTMVSRG
                     LGLVPTLSEYLHEF"
BASE COUNT          755 a         1576 c         1502 g          916 t
ORIGIN      
        1 gtcggtgagg gtgtcggcgc ccaccaattc cccgatcaac tcccgtcccg agcccgtcgt
       61 acccacgccc cgaatccgga tgcggtcgcc gagggcggtc acgatcgtac ggaggccctg
      121 gctcaccact tgcgtgggcc ggccctcggt ccagacgtag atctcgtgca tgacctcggc
      181 ggcgtcgctc aggacgacca ggttcgtgct gaccgagccc acgtcgatcc ccaggtaggc
      241 gtcctgcacg gagcggtcct cgggcagtcg gtacggctgc acccggtccc ggaggaggag
      301 cacctgctcc aggttgaggg ggcgggacgt ggccccatca ccgttcccct ccgtcggcgt
      361 tgcgcttggt cggcgactcg gccacgcccc cgtccgtcgg ccatttccca ccgctcccag
      421 cagcgccgcg ccgatcgccc cgaaccaggc ataggcctcg ggaacgaaga gctgccgctc
      481 gtcgaaatgg aacagctctc gcaaggcccg gatcacgcct gcgttcagag cgaggccgcc
      541 gatgaaggcg accggcggca cggcgggctt ccccttggtg atgctgccct tgaagttcct
      601 cgccacggcg tcgcacagcc cgcggaggat ctggggcgga tcggcacctt tctgctgggc
      661 atgaatcatg tcggacttgg caaagacgga gcagcgaccc gcaatccgcg ccgcagaggg
      721 cgcggcacag gcgatacggc cgatctcctc gatctggtag cggaggcgcg tggcctgttg
      781 gtccatgaat gcaccggtgc cggccgcgca gtccccgttg gtctcgtagt cctggaggcg
      841 agcctgccgc gtactcccat tggatttgag ctggagaaac cgagacgatt gcccgcccat
      901 ttcgaagacc gtggccacct ccggatgcaa cagtcccatg gccatcgcga tggctttggc
      961 gtcgttcacc ctggggatcc cccggcgcgc ctggagcagt cccgctccgc aaccggtagc
     1021 ccccacgtga cccggaggca gcccagggat cagccgcagg acattggcca agagcgcctc
     1081 ggcgacgccc agcgggtcgc ccagggcgcg acagggagag gaaacgagga tgggatcggg
     1141 gaaggataac caggcgaagt cgggggtcag ggctaaactt tccagcatgg gacgatcccc
     1201 cgccgtcccc aggaccgccg cctttacact gatggctccg atgtcgatgc cgacgctgat
     1261 ggccatgact ggctcacgcc gacttgaccc gcgaagtgat tttgccggtc aagcaggacg
     1321 ggtgtcggct gggcagggag aatgtggggt cacccaagtg gcccatgccc gagaaagtgt
     1381 gcaagcgctg gatcgtcctc ctcaatcaag gtcgatgtcc cgaatgggct tggtgatagg
     1441 gacgtctttc gttcgcttga gtgcatcctg gaatttcttg tccaggagat ctttcttgcc
     1501 cttctcggct ttcatggatt ccacgaactt ttcctgccgg tgggcggctt cggccttcag
     1561 accctttacc gcctcggtca gatccttcac cgagcgctcc tccggcggca gctcgtgcga
     1621 cagcaccgcc cgcagcgccg ggtccacccg gagttttgcc tggcaacacg gacacacgac
     1681 ttcaaaaggt tcgaaggctt cctggctcat ggcgacctcc acgcgttacc cagggcttat
     1741 ttaccaggag cggccgtgaa gatcaagccc gaattccacc ggccgctgtc ggaagcgccc
     1801 aggcccttcc cgggcgcttc cctggaatcg cgagtcgagg tatagtcccc atattttccg
     1861 gaaggaggaa tctgcgtgaa ccccgcaaga cgcgctctag acggccgcgc catgatcatc
     1921 gtggtgaccc tttgcttcct ctggggactc tcccaggtgt ccatcaaggt ggccaatggc
     1981 ggcgtgagcc ctgtcttcca ggccgcggtg cggtccgtcg tggcgagcat tctggtggcc
     2041 ctatggtccc gtgcgcgtgg cctgcccttg atccatcggg atcggaccct cttccatggc
     2101 ctggcggtcg gcctcctgtt cgggacggag ttcgtgtgca tctatctcgg tttgaactac
     2161 accacagcct cgcatgccgt gatcttcctc tatgccgcac ccttcttcgt ggcgctgggc
     2221 gcccactggc tcctccccca cgagcccctt accccccgga aacttgcggg cctcaccttg
     2281 gcattcctcg gtgtggccgt cacgttgcgg gagagcctcg gcacacccac ctcgctacag
     2341 gtcgtcggcg acctcctgag tctcacgggc ggattcctct gggcggcaac ctccctttac
     2401 ctcaagcgcg tcgtccacac gagcatgacc ccttcccaga tgctctttta ccaactcggc
     2461 gtttcggcgc tgcaactctc cctgatgagc gccatcttcg agccgcaccc gatccgggag
     2521 ctgagtcctc tggtcctggg atgcctcgcg taccaggcgc tcatcgtggc cttcgccagt
     2581 tatctcgtct ggttctggct gatccaggtt taccccgtga gcaacctctc gggcttcacg
     2641 ttcttcacgc cgatcttcgg ggtgctcctg ggtgggctgc tgctgcacga gcctctcacc
     2701 cccagcctgc tggcgggtgc ggcactcgtg gccggtggaa tgattctggt gaattggccg
     2761 gagaagatag gggggaagcg tggggagcgg acctgagcat cccgttaggt gtgaatcggg
     2821 agacgaacgg tgatttttgc ttgacggcct tcgggataca gcctagtctt ccggcgatcc
     2881 caacgatctc actgtctagt cttcggggag gggcacgctg tccttggttc tggcccgtga
     2941 gaaggctgtt cacgacttcg tcgctcttgc cgatggtcgg cagggagatt gctgagtctg
     3001 ccgccagacc tttacatcgc tccacgccct tccctgcctc ggatctggag gccaaggagc
     3061 cccgtccacc gaaagctgga tgggggtgcc ttcctagcgt gcctgctttt tacctgactt
     3121 ggattcaccg gatagaaccg aggaagcgtc ccccagcgcg tacctgaaag cggggttgcg
     3181 catgcaggcg accacggaga aggttgcgcc agcctttcgc ggcagcatca gccgtcgatt
     3241 caccatcctg ttcgttcatt tcctcgcgtt cgtcgccgtc gtcggcgctc tgtcggttta
     3301 caaggccaac cgcattgcgc gcctccacac cgagatcgga gaactcggcg cacatatcga
     3361 ggtcctggat cggctggaga ccgctatctt ccggctgcgg gccgagagcc aagattcgtt
     3421 gttcctggga gaggcgtttc acgatccctg gctcgatgag attccccgtc agatccggaa
     3481 acagtggagg catttcgaac agttgcactc ccatgacggc catccatggg agaccggacc
     3541 ctcgctcgag cacgccctct accaggagct ggacccgcgc ctcgacacgt tcattgccat
     3601 agcacggggt gccgcgaaag gaacccgccc cgagggttcg aaactgcggg aggcggcgtt
     3661 cgtgttggac gccgaggcgc atgccctcga agatgggttg gagcggctgt cggcccttca
     3721 tcgaggcctc atggccgatc gatcgcgggc cgccggggcc gacgcggatt tcattctgtg
     3781 ggccaacgcg agcttcatcg cttttcttgg tctgaccgtc gttgttgcga gcagcctgtt
     3841 cgtgcgccgc gtggccctcc cgctccgacg cctgaatatc gcggcgctgg atgtagccga
     3901 tggacacttc ccgcttcagg tgccggtcaa atcacgggac gagatcggca gactggccga
     3961 caccttcaac cacatgtccg aagccctctc cgagcgagac gctcagttac gccggcgcgc
     4021 gcaagagatg gaggccctct cccgtatcgg caccgatatc tcctcgatgc tcaatctgga
     4081 ccgcctcctc caaacgattg tggacagcgc ccgcaccttg ctccggaccg acgtagccgc
     4141 cctctgcctg ttgcgagacg gcggtcccgc cctggatgtt gtggcgacag ccgggccgtg
     4201 gaggcccgtg atgggctcgg cgccggcggt ctccacggac gacctgggaa ccgattgcga
     4261 tgagatctgc gtgcgctgcc aggaggctcg ctcgacggct gggatggcgc agctctcggc
     4321 tcgcctcaag ctgggcgaca agccactcgg cgcactctgc gtcggcagta caggtcaccg
     4381 cgccttcggc gaggtcgagc ggaacgttct aagcggcctg gctgcccagg cggccatcgc
     4441 catcgggaac gcccgcttgt acgagcgggc ccggggcatg gccgcggtgg aggagcgcga
     4501 gcggctggct cgcgagatgc acgatggtct ggcccaggcg ctgtcgttcc tcaacttcaa
     4561 gctggccttg acggaccgct tgctggacgg cggaccggtg gcgccggttc atcaggaggt
     4621 ggccgagatg cagaaggtga ccgccggggc gtacgcggag ctccgccagg ccatcttcgg
     4681 cctgcgaacc atggtgtccc gcggtctggg gctggttccc accctgtccg aatacctcca
     4741 cgagttcag
//
LOCUS       DBNGZC010000135         4055 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_499768, whole genome shotgun sequence.
ACCESSION   DBNGZC010000135 DBNGZC010000000
VERSION     DBNGZC010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_499768"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="AC16G0_02595"
     CDS             <1..234
                     /locus_tag="AC16G0_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR5098540.1"
                     /translation="IRDNTLTLRGERRVAKDVQEENYLRIERAYGSFQRSFTLPTTIQ
                     QEKVRAVFKDGVLELMLPKAEGAKPKKIAIEVR"
     gene            307..768
                     /locus_tag="AC16G0_02600"
     CDS             307..768
                     /locus_tag="AC16G0_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR5098541.1"
                     /translation="MGSQPWPGPEWYPVGDLAVIKQEMNRLFESFLGLSPGHLQPGEG
                     TWAPPMDVCETRDEIRVLLELPGVSQSQIKLEIVDGVLSIKGERQPDPGFRQDQLLRM
                     ECHHGPFARRLNLPSVVDPDGVRATYRNGVLEVRLPKRAEARAQSITVEAT"
     gene            804..1919
                     /gene="dnaJ"
                     /locus_tag="AC16G0_02605"
     CDS             804..1919
                     /gene="dnaJ"
                     /locus_tag="AC16G0_02605"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR5098542.1"
                     /translation="MAKRDYYEVLGVRRGASEQEIKRAYRKLARKHHPDVNPGNKLAE
                     SKFKEITEAYEVLSDPVKRRRYDQLGPDGFAAGAGARGGEPGAGPGGFDFSRVDFGTS
                     GFGDLGDIFSDLFGQRGSPQSAAPGKGEDLHYSLDIDFEDAIHGLSTEISIRKHSPCA
                     VCRGTGAKPGSPLETCPDCGGSGRRQTRGFLRSQQPCPRCQGNGKISREACVTCAGRG
                     VTFGTERISVKIPAGVDTDSRVRLQGMGEPGQNGGPPGDLYIITRVRPHPVLERKGDN
                     LQVEVPITITEAALGARIEVPTTDGTTTMRIPPETSSGQVFRLRGKGVPHLKGGGQGD
                     QFVTVKIVAPKNLDTRSQELLREFARLNPGDPRAGRG"
     gene            1959..2360
                     /locus_tag="AC16G0_02610"
     CDS             1959..2360
                     /locus_tag="AC16G0_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein transcriptional repressor
                     HspR"
                     /protein_id="HIR5098543.1"
                     /translation="MVKEKRPLYMISVVAEMFEIHPQTLRAYEREGLLRPARTDGNTR
                     LYSQEDLERIELILRLTKELGVNLAGVEVILNMRERMDEMRQQMNEMFQQVFRRMETE
                     FKHMEQEQQNAEGLVPVGRKGIVRQVHVEEG"
     gene            2487..3344
                     /gene="htpX"
                     /locus_tag="AC16G0_02615"
     CDS             2487..3344
                     /gene="htpX"
                     /locus_tag="AC16G0_02615"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012468638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HIR5098544.1"
                     /translation="MSNTLKTTALLAALTVLLILIGSLVGGEQGMVVAFVFAGLMNFA
                     SYWWSDKIVLWMYGAREVSEAEAPGFHALVRRLAQRAGLPMPKVYIIPTDTPNAFATG
                     RNPQHAAVAATEGILRLLTSDELEGVIGHELGHVRNRDILTSTVAATLAGAIMMLSRM
                     AQWAAIFGGGRSRDDDEGGAGGIIGLLALAILAPLAAMLIQMAISRAREYQADATGAQ
                     ISHKPWALADALEKLEHASTAIPMDANPATAHLFIVNPLSGSSILNLFSTHPPIEARV
                     ARLRAMRIA"
     gene            3367..3999
                     /locus_tag="AC16G0_02620"
     CDS             3367..3999
                     /locus_tag="AC16G0_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098545.1"
                     /translation="MGKRGRVARAFTFGLGLFLAGCASEPKGSILAIQPGPQQQRTHQ
                     VVGDAIVASLGGATVTVRWLDAKGVEKYFQSRPGLVYPWPKEIWKENPPTIFHLQVRS
                     PREEVQFDPGLASLVTKGGRRERLIPYEEMYMDMAGEEGSGPRLLSLQAALFSRFVVI
                     PPGGQREGLLVFRPLDPETMYLILELSSFFVGGRVIPGLFEFQVLREKSE"
BASE COUNT          743 a         1232 c         1410 g          670 t
ORIGIN      
        1 atccgggaca acaccctgac cctgcgaggc gagcgacgcg tcgccaagga cgtccaggaa
       61 gagaactacc ttcggatcga gcgagcctac gggagcttcc agcgcagctt caccttgccc
      121 acgaccattc agcaggaaaa ggtccgggcg gtattcaagg acggcgtgct ggagctcatg
      181 ctgccgaagg cggaaggggc caagccgaag aagatcgcca tcgaggttcg ctgacagcgt
      241 gatggggggc ggtacaccgc gctatcggcc acggcgaggc cgcaccacag caaaggggga
      301 cgtcgcatgg gatcccaacc ttggccgggg cctgagtggt atccggtggg tgacctggcc
      361 gtcatcaagc aggaaatgaa tcggctcttt gaaagcttcc tgggtttgag tcccggtcat
      421 ctccagccgg gggaggggac ctgggcgcct cccatggatg tctgcgagac ccgggatgag
      481 atccgcgtgc tgcttgagct tccaggggta tcccagtccc agatcaaact ggagatcgtg
      541 gatggtgtgc tcagcatcaa gggcgagcgt cagcccgacc cggggttccg ccaggatcaa
      601 ttgttgcgga tggaatgtca ccacggcccc ttcgcacgtc gtctcaacct cccatcggtc
      661 gtcgatccgg atggcgttcg ggccacctat cggaatggcg tcctggaggt ccggctgccc
      721 aagcgggccg aggcgagggc tcaatccata acagtggagg cgacatagcg ggccggccgg
      781 cgcgatgcct tcgggagtag ggaatggcga agcgggacta ctacgaggtc ctcggggttc
      841 gccggggagc ctcggagcag gagatcaagc gggcataccg gaaactggcg cggaagcacc
      901 atcccgacgt gaatccggga aacaagctag ccgagagcaa attcaaggag atcaccgaag
      961 cctacgaggt gttgagcgat ccggtgaagc gccgccgtta cgaccagctg gggcccgacg
     1021 ggttcgctgc cggggcggga gcccgaggag gcgaaccggg cgccgggccc ggcgggtttg
     1081 acttcagccg ggtggacttc gggacgagtg gatttggcga tctgggggac atcttctcgg
     1141 atctcttcgg gcagcggggg agcccccaga gcgctgcgcc ggggaagggg gaggacctcc
     1201 actatagcct ggacattgat ttcgaggacg ccattcacgg cctctcgact gagatcagca
     1261 tccggaagca ctccccctgt gccgtctgtc gggggacggg ggccaagccg ggcagccccc
     1321 tcgagacgtg ccccgattgc ggtggcagtg ggcgacgcca gacccgggga ttcctccgca
     1381 gccaacagcc ctgcccgcgc tgtcagggca acggcaagat cagccgggag gcctgcgtca
     1441 cctgtgccgg ccgaggggtc accttcggga ccgagcggat ctccgtgaag attcccgcgg
     1501 gggtggacac cgactcacgg gtccgcctcc agggcatggg ggagccaggc caaaacggcg
     1561 ggcctcccgg cgacctctac atcatcacgc gtgttcgccc gcacccggtg ctggagcgga
     1621 agggtgacaa tctccaggtc gaggtcccca tcaccatcac ggaagccgcc ctgggcgcgc
     1681 ggatcgaggt cccgacgacc gacggcacga ccacaatgcg aatcccgccg gaaaccagca
     1741 gcggacaggt cttccgctta cgggggaagg gggtccccca cttgaagggc gggggacagg
     1801 gcgaccagtt cgtgaccgtc aagatcgtgg cgccgaagaa cctggacacg cggtcccagg
     1861 agttgctgcg ggagttcgct cgactgaatc ccggcgaccc acgggccggg cggggataga
     1921 ttctggaggg gcgggcctcg gcccgcgatc gattgcctat ggtcaaggaa aagcgaccgc
     1981 tgtacatgat cagcgtggtg gcggagatgt tcgagatcca cccgcagacg cttcgtgcct
     2041 acgagcggga aggcctgctg cgcccggcca ggaccgacgg gaacacgcgc ctgtactctc
     2101 aagaggatct ggagcggatc gagctgatcc tgcgcctgac caaagagctt ggggtgaatc
     2161 tggccggggt cgaggtgatc ctcaacatgc gggagcggat ggatgaaatg cgtcagcaga
     2221 tgaatgagat gttccagcag gtcttccgtc ggatggagac ggagttcaag cacatggaac
     2281 aggaacagca gaatgcggag gggttggtcc cggtgggccg caagggaatc gttcgccagg
     2341 tccacgtcga agagggatga gcggaacccc atggtccagc ttgacagcgc ggggatccct
     2401 cctctattct gccctggaaa gcattcggat gagcgagata ctcaggaaaa ccgcagcctc
     2461 gggagcgcgg tgaaggagtt tgagtcatgt ccaacacgtt gaagacgacg gccctgctgg
     2521 ccgccctcac ggtgctgctc atcctcatcg gcagcctggt gggaggggag caggggatgg
     2581 tggtggcctt tgtctttgcc ggcctcatga atttcgcctc ctactggtgg tcggacaaga
     2641 tcgtgctttg gatgtacggc gcccgcgagg tgagtgaagc cgaagcgccg ggattccacg
     2701 cgctggtccg caggctggcg cagcgcgccg gcctccccat gccgaaggtc tacatcatcc
     2761 cgacggacac cccgaatgcg ttcgccacgg gacgaaaccc gcagcatgcc gccgtggcgg
     2821 ccaccgaggg gatcctccgc ctgctgacct ccgatgagct ggagggggtg atcgggcacg
     2881 agctgggcca tgttcgcaat cgggacatcc tgacctccac cgtggcagcg accctggcag
     2941 gggccatcat gatgctgtcc cggatggcgc agtgggcggc cattttcggt ggcgggcgaa
     3001 gcagggacga tgacgagggg ggtgcggggg gaatcatcgg gctgctggcg ctggccattc
     3061 tggcgcccct ggcggcgatg ctgatccaga tggcgatctc ccgggcgcgc gaatatcagg
     3121 cggacgccac gggcgcccag atcagtcaca agccctgggc cctggccgac gccctggaga
     3181 agctggaaca cgcctccacg gcgatcccca tggacgccaa tccggccacc gcccacctgt
     3241 tcatcgtgaa tccgctgtcc ggctccagca ttctgaatct gttctccacg cacccgccca
     3301 tcgaggcccg cgtggcgcgg ctgcgggcca tgcggatcgc ctgacgtgat gccgtcggga
     3361 cggatcgtgg gaaagcgcgg gcgtgttgcc cgcgctttca cttttgggct ggggctgttc
     3421 ctggcggggt gtgcctcgga gccgaagggc agcatcctgg cgattcagcc ggggcctcag
     3481 cagcagcgca cccaccaggt ggtcggcgac gccatcgtcg cctccttggg tggtgcgacg
     3541 gtgacggtac gttggttgga tgccaagggg gtggagaaat acttccaatc gcgacccggc
     3601 ttagtgtacc cgtggcccaa agagatctgg aaagagaacc cgcccactat ttttcacctg
     3661 caggtccgga gcccgcggga ggaggtgcag tttgaccccg ggttggccag cctcgtgacc
     3721 aagggcgggc ggcgcgagcg cctcattccg tatgaggaga tgtacatgga catggcgggg
     3781 gaggaaggct cgggtcctcg cctcctcagc cttcaggcgg cgttgttcag tcgctttgtg
     3841 gtcattcctc ccggcggcca gcgggagggc ctgctggtgt tccgtcccct ggacccagag
     3901 acgatgtacc tgatcctgga gctatcgtca ttcttcgtcg ggggccgcgt gatccccggc
     3961 ctcttcgagt tccaagtcct ccgcgaaaag tccgaataaa gcccaccgca gatttcgtgg
     4021 tttccgcaga gaacaccctc accctgcctc ttccc
//
LOCUS       DBNGZC010000136         2628 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_793852, whole genome shotgun sequence.
ACCESSION   DBNGZC010000136 DBNGZC010000000
VERSION     DBNGZC010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_793852"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="AC16G0_02625"
     CDS             complement(<1..476)
                     /locus_tag="AC16G0_02625"
                     /inference="COORDINATES: protein motif:HMM:NF024241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5098546.1"
                     /translation="MSEPYLTPFWRSLGFSPTQLGLLNAIAPGVAAIAPFLWTIYADV
                     TRQGERIFLWNTWAAALTAFLLPNLQAFLAVAMAMLIYSTFRMALIPLANSMTFQALA
                     GRRQGYAAIRLWGTVGYILVAVAAGAGMDRLGLRAGIYGIAVTMLACAVAAWLGQRR"
     gene            complement(619..1443)
                     /locus_tag="AC16G0_02630"
     CDS             complement(619..1443)
                     /locus_tag="AC16G0_02630"
                     /inference="COORDINATES: protein motif:HMM:NF024587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HIR5098547.1"
                     /translation="MTIVAVSREMGSGGYEIASAVAKALDFEYVDREILLQAARAHAV
                     PEEKIADVDERRLSLWERFDEEKRRYLIYIEAAYYSFAEKGRVVTAGRGGPFFLRDVT
                     HALKVRIMAPVDVRVRRVMAQEKLDSKTALARVKNYDRELSGRIDYLFGLDWALPEHY
                     DLVINTGGDTWEFYVDLLVSAAQHPQFQPTPESKQKIRDLSLAAHVRAAIANDPVTKN
                     INVEVTAQSGRVALKGVVFSPAMMDAAAEVANRVPGVSEVSCEAVEIPRVYPGPIM"
     gene            complement(1531..>2628)
                     /locus_tag="AC16G0_02635"
     CDS             complement(1531..>2628)
                     /locus_tag="AC16G0_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR5098548.1"
                     /translation="SADGYILTNQHVVEQASDIEVQLMDDRKFPAKIVGRDVRTDLAL
                     LKIEATGLPPLPLGDSDKLEVGELVLAIGNPFGLEYSVSLGIVSRKGRALGGSGAFDD
                     YIQTDASVNPGNSGGPLLNMRGEVIGINTAVIPNRRVAFAIPVNLAKTLLPDLQAKGR
                     VAWGFLGVSIQDLNRELATALGVQDTKGALVNNVLSGQPADAAGLKRGDVIVQFDGKP
                     VPNVRALQRAVSFTPVGKQVELQILRQGKLEAIHAKVGEAATAERQASVARPQRDMGM
                     MVEELDTEKAKKFKLREDEEGLVVTDVTRGGPASGAGVRAGDVIREVNRRPVRSLDGY
                     RGALRGGDGETDLFLLKRGEAYVYVAVKPKT"
BASE COUNT          417 a          919 c          855 g          437 t
ORIGIN      
        1 cggcgctgcc ccagccaggc cgcgacggcg caggcgagca tggtcacggc gatgccgtag
       61 atccccgccc gcaagcccag ccggtccatg ccggccccgg ccgccacggc caccaggatg
      121 tagccgacgg tcccccacag acggatggcc gcgtacccct ggcgtcggcc cgccagcgcc
      181 tggaaggtca tgctgttggc gagggggatg agcgccatgc ggaaggtcga gtagatcagc
      241 atcgccatcg ccaccgcgag gaacgcctga aggttcggca agaggaacgc cgtgagggcc
      301 gcagcccagg tgttccagag gaagatccgc tcgccttggc gggtcacgtc ggcatagatg
      361 gtccagagga agggggcgat ggcggcgacg cctggtgcga tggcgttcag gagaccaagc
      421 tgcgtggggg agaaccccag ggaccgccag aagggagtga ggtacggctc ggacacgccg
      481 atggcggcga agtaccagaa gtagtacgcg gcgaggtacc gggtgctggt caggcgggtc
      541 tcctcgaggc gaatctatca gcggtcagcc atcagcgtcg gtccctggct gagagcggaa
      601 cgctgatggc tgttgtcttc acatgatcgg acccgggtag acccgcggga tctcgacggc
      661 ctcgcaggaa acctcgctga cgcccgggac gcgattggcc acctcggcgg cggcatccat
      721 catggccggg ctgaagacca cccccttcag cgccacccgg ccggattggg ccgtcacctc
      781 gacgttgatg ttcttggtca cggggtcgtt ggcgattgcg gcccgcacgt gggccgcgag
      841 actgaggtcg cggatcttct gtttggattc cggcgtgggc tgaaactgcg ggtgctgagc
      901 cgccgacacc aggaggtcca cgtagaattc ccaggtatcc cccccggtgt tgatcaccag
      961 gtcgtagtgt tcggggagcg cccagtccag cccgaagagg tagtcgatgc gtccggacag
     1021 ctcccggtcg tagttcttca cgcgggccag ggccgtcttg gagtcgagtt tttcctgggc
     1081 catgacccgc cgtactcgga catccacggg ggccatgatc ctgaccttga gggcgtgggt
     1141 cacgtcgcgg aggaagaacg gacccccgcg tccggcggtc accaccctgc ccttctcggc
     1201 gaaggaatag tatgccgcct cgatgtagat gaggtagcga cgtttctcct catcgaagcg
     1261 ctcccagagg gacaggcgtc gctcgtccac gtcggcgatc ttctcctcgg gcaccgcgtg
     1321 ggcccgggcc gcctgcagca ggatctcccg gtccacatac tcgaagtcca gtgccttggc
     1381 aacggcggag gcgatctcgt atccgccgct tcccatttcc cgggacaccg ccacgatggt
     1441 catggcccca cctcatccgg atcgtgccga caggaccctg ccccgggtcc ggagcattgc
     1501 cccattgccc gccgggccgt gcgccgcgcg ctaggtcttg ggcttcaccg caacgtatac
     1561 ataggcctcg ccgcgcttga ggaggaagag gtccgtctcc ccgtctcccc cacgcagggc
     1621 gccgcggtac ccgtccaggg atcgcaccgg tcgccgattc acctcgcgga tcacatcacc
     1681 ggcacggacc cccgcgcccg aggccgggcc gcccctggtc acgtcggtga ccacgagtcc
     1741 ctcctcatcc tcccgcagct tgaacttctt ggccttctcc gtgtccagct cctccaccat
     1801 catccccata tcccgctggg gccgcgccac cgaggcttgg cgctccgccg tggcagcctc
     1861 gccgaccttg gcgtggatcg cctccagctt tccctggcgc aggatctgga gctccacctg
     1921 cttgcccacg ggggtgaagc tgacggcccg ctgcagcgcg cggacgttcg gcacgggctt
     1981 gccgtcgaac tgcacgatca cgtccccgcg cttgagcccg gccgcatccg ccggctgccc
     2041 ggagaggacg ttgttcacca gcgccccctt cgtgtcctgg acgcccaggg ccgtggccag
     2101 ctcccgattc aggtcctgga tgctcacacc caggaacccc caggcgaccc gccccttggc
     2161 ctgcaggtcg ggaagcaggg tcttcgccag attgacgggg atggcgaagg ccacccgccg
     2221 gttgggaatc acggcggtgt tgatgccgat cacctcgccc cgcatgttga gaagcgggcc
     2281 gccgctgttg cccggattca ccgaggcgtc ggtctggatg taatcatcga aggcgccgct
     2341 gccgcccagg gcccgcccct ttcggctgac gatgcccaga ctcaccgaat actccaggcc
     2401 gaagggattc ccgatggcca gcaccaattc gcccacctcc agcttgtccg aatcgcccaa
     2461 gggcagcggg ggaagccccg tggcctcgat cttcagcaac gccaggtccg tccgcacgtc
     2521 gcgacccacg atcttcgcgg ggaacttccg gtcatccatg agctggacct cgatgtcgct
     2581 ggcctgctcg acgacgtgct gattcgtcag gatgtacccg tccgcgct
//
LOCUS       DBNGZC010000137         3191 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1185137, whole genome shotgun sequence.
ACCESSION   DBNGZC010000137 DBNGZC010000000
VERSION     DBNGZC010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1185137"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /gene="ruvX"
                     /locus_tag="AC16G0_02640"
     CDS             complement(<1..410)
                     /gene="ruvX"
                     /locus_tag="AC16G0_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR5098549.1"
                     /translation="MKILAVDWGRARLGLAVSDDLGVTAQALVSLPRVGESQDVEAIG
                     LYIRRLGVEAIVVGLPKNMDGSLGPSAEAAQSFAQCLHQRLGIPVHLWDERLTSAEAN
                     RVLRASEMSIKRRGEVVDKLAAMLILQSWMEARK"
     gene            complement(925..2784)
                     /locus_tag="AC16G0_02645"
     CDS             complement(925..2784)
                     /locus_tag="AC16G0_02645"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR5098550.1"
                     /translation="MVRSRGRKNGTSFYQLDCIACGRHVKETESSTRCPACHQPLTVR
                     YDYDYIRSRLNRYSLRTSPVKALKYLDFYPLLDLDLVVSMNEGGTPLYRCTRLAERVG
                     LRHLFIKNEGANPTGVFKDRGSLVELTKAKEQGAKAVCVASTGNMAASVAAYASVAGL
                     PCYVLVPEGTAIGKMAQALSYGARLLQVRGTYTEASRLTEEMSQRHGFYLAGDYAFRV
                     EGQKSQAFEIIEQLDWQAPAAVIVPMGCGTNMAAVWKGFKEFYELGLIDRLPRMVGVQ
                     PDGCAPIVGAFQQGTDTVTVVEKPITVCTAVAAGDPVDGIKALAAMRESGGCAVILTD
                     AEILEAQQQLAHLESIFVEPAGAIPIATLGALLTTGRIRPDEMVVCLATGNGLKDPKA
                     ALRVLPSPATIEPTTAEVDKFLRLKLYEIRAAAAKNGARSLFDQVPSQRLVADTVRQE
                     LGVKLTAEYAHRVTAMVTDFVKKGKVITKGDLQYIVETVLKALSEPQPILQVEDFQVV
                     TSLKGQAEAKVWITFDAERVTSAANGVGPVDAVVNALKTAAESRGKLFFDLVDFQVEI
                     NSPGTDASVEATITMKDTKNNRVVATGTSPDVIVASVNAFVEGYNMLWSRQKA"
BASE COUNT          588 a         1058 c          939 g          606 t
ORIGIN      
        1 tgcttcctgg cttccatcca actctggagg atcaacatgg ccgccagttt gtctacgacc
       61 tcgccccgtc tcttgatcga catttccgac gcccgcaaaa cacggttggc ttccgccgag
      121 gtgaggcgct cgtcccacag gtgcaccggt atgcccaagc gctggtgcag acactgcgcg
      181 aaggattgtg ccgcctcggc cgatggaccc aaactcccat ccatgttttt cggcagaccg
      241 accacgatgg cctcgacccc caagcgccgg atgtagagcc cgatggcctc gacgtcctgg
      301 gattccccta cccgtggcag gctgaccagc gcctgggcgg tgacgccgag gtcgtcgctc
      361 actgccaggc ccagcctggc ccgcccccaa tccaccgcaa ggatcttcac cactcaagcc
      421 tcattccccg accgctggcg acatgactcc tcgccgggat ggcggcaggt tcctgccccc
      481 ttgccgccca gtcgccctgt gctgccggtc gtccgcgagg actcccgggt cgccggcgag
      541 gctctgctcc gtgtatcggg tgacattcag ccggctggtc cctcaaaggc ggtgaccggg
      601 ccggactgga ttcgaggtga tccttggggc aatatcgacg gagaaaaccg cccggactaa
      661 cgtgacccag gcgattgcga atttccagga agcgcgggtc ttgacaccga gaaggttggt
      721 cggactccgc accgacccac cggcctcaaa cgctcaacgt ggggagcatt ataccatacg
      781 gaccagaggc ctccaaagct tccgtgccag cctgcgcgtc tatgcctgat acgggcgagt
      841 cgggaagtcg cctcccccgg cacaacagcc cattcccgca cctgccgtcg ttcagcggct
      901 ctccgcccag gaccggctac gccctcacgc cttctgcctt gaccacagca tgttgtaccc
      961 ctcgacgaag gcgttcacgg aggccacgat gacgtccggg gaggtaccgg tcgccaccac
     1021 ccggttattc ttcgtatcct tcatcgtgat cgtggcctcg accgaagcgt cggtcccggg
     1081 cgagttgatc tctacctgga agtccacgag gtcgaaaaac agcttgcccc ggctttccgc
     1141 cgcggtcttc agggcattca ccacggcatc caccggccca acaccattgg ctgcggaggt
     1201 cacccgctcg gcatcgaagg tgatccacac ctttgcctcc gcctgcccct tcaacgaggt
     1261 gacgacctgg aagtcctcga cctggaggat gggctgcggc tccgaaaggg ccttgaggac
     1321 cgtctccacg atgtactgga ggtcaccctt ggtgatgacc ttccccttct tcacgaagtc
     1381 ggtcaccatg gccgtcaccc gatgggcgta ctcggccgtc aacttcactc ccagctcctg
     1441 gcgaaccgtg tcggccacca ggcgctggga cggaacctga tcgaagaggc tgcgggcgcc
     1501 attcttggcc gcggcggcgc ggatctcgta gagcttgagg cgcaagaact tgtccacctc
     1561 tgcagtggtg ggctcgatgg ttgcgggtga cggcagcacc cgcagggccg cctttggatc
     1621 cttgagtcca ttccccgtgg ccaggcagac gaccatttca tcgggacgaa tccgccccgt
     1681 ggtcaggaga gctccgaggg tggcaatggg aatcgcgccc gctggctcca cgaagatgct
     1741 ctccagatgg gccagttgct gctgggcctc caggatctcc gcatctgtta ggatgaccgc
     1801 gcaccccccc gattcccgca tggcggccag ggccttgatg ccatccaccg ggtccccggc
     1861 agccacggcg gtgcaaaccg tgatcggctt ctcgaccacc gtcaccgtgt cggtgccttg
     1921 ctggaacgcg cccacgatgg gggcgcagcc atccggttgc acgcccacca tccggggcag
     1981 acgatcgatc aggcccagct cgtagaactc cttgaatcct ttccataccg cggccatgtt
     2041 cgtgccgcag cccatgggaa cgatgactgc ggcgggcgcc tgccagtcca actgctcgat
     2101 gatctcgaag gcctgtgact tctgcccctc cacccggaaa gcgtaatcgc cggccagata
     2161 gaacccgtga cgctggctca tctcttccgt gagacgtgac gcctcggtgt aggtgccccg
     2221 gacctgaagc aggcgcgccc cgtaggagag cgcctgggcc atcttaccga ttgccgttcc
     2281 ctcgggcacc agcacgtaac aagggagacc cgccacggag gcgtaggcgg ccacggaggc
     2341 ggccatattc ccggtggagg ccacgcacac cgccttggcc ccctgttcct tggccttggt
     2401 gagctccact aggctccccc ggtctttgaa caccccggtc ggattcgccc cttcgttctt
     2461 gatgaagaga tgccggaggc caacccgctc agccaggcgg gtgcagcggt acaggggggt
     2521 tcccccctcg ttcatcgaga ccacgagatc gagatccagc agggggtaaa agtcgaggta
     2581 cttgagcgcc ttgacgggtg aggtgcggag ggagtaccga ttcagtcgcg accggatgta
     2641 atcgtaatcg tagcggacgg tgagcggctg gtggcaagcg gggcagcggg tgctactctc
     2701 cgtctccttg acgtgcctgc cgcaggcgat gcagtcgagt tgatagaagc tggtgccgtt
     2761 cttccggcct cgacttcgga ccatgttccc tcacatgcga gaaaatccgc tggagtctag
     2821 ccgtcatgtc ccatgatgtc aaggatttcc ccgatcaacg tcccccgagc aaagccgctt
     2881 ttctgggccc gtaagaggca tccattgagc ccgcgtggag ccgcctgcat acgactgctg
     2941 ggcataacgg ggaactactt ctgtggcaaa tgctaagatg gcaatgaagt gaatatactt
     3001 ccttggttga agttggaaga attcaagcac ctaatacaca aagaaagtat cgtaactaac
     3061 taacaaaaaa tagatttatg gtcttgtgag ttgtttgctg acggacccat tgaagagcaa
     3121 ctgaccccgg ccactcattc gtagccagcc tcggacaact cgtcctcatc cagtccgaag
     3181 tggtggccga t
//
LOCUS       DBNGZC010000138         4389 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2457109, whole genome shotgun sequence.
ACCESSION   DBNGZC010000138 DBNGZC010000000
VERSION     DBNGZC010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2457109"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="AC16G0_02650"
     CDS             <1..581
                     /locus_tag="AC16G0_02650"
                     /inference="COORDINATES: protein motif:HMM:NF014517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HIR5098551.1"
                     /translation="RWPTWSRSTYNATYPITHQDGHYLRSYGKEPLMKDLLPLAEKVS
                     ALLKSRRETVAVAESSAGGLISAGLLAVPGASTYFLGGAVVYTYGSRGVFLDVPEAAL
                     RDIRPATEAYALLLARSVRQRLAATWGLAETGATGPTGNRYGDPAGHACLAVTGPAER
                     AITLETGRADRLANMHAFATATLDLFAQLLAR"
     gene            complement(789..1946)
                     /locus_tag="AC16G0_02655"
     CDS             complement(789..1946)
                     /locus_tag="AC16G0_02655"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015858630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5098552.1"
                     /translation="MIHDSDSPTTSATSPGGPREAHIEVRDLVVQYGHVPAVRGVSFT
                     VRPGEQVTLLGPSGCGKTTTLRAIAGLEPPTSGKIVIGGAAVFSSAERINVPAERRGL
                     SMVFQSYAIWPHMTVFDNVAYGLRVRRLGTREIAEKVRWALDMVQMGGFAERSASKLS
                     GGQQQRVALARAFVFSPSVLLFDEPLSNLDAKLRAEMRIELRELQHRVGITSVYVTHD
                     LEEALAISDRIAVMRDGAIEQIGTPSEIYNFPRNAFVADFVGSANLIRGRHRPECGRD
                     GLIALETSGGTIVYGTAHGRPVGPDGVFSVRTIHLGLSREAPAAQANVWSVRIRRRVF
                     QGDFTQYHVDWENRQLVVRCAATELLEEGDQAYLTVDPRRCVLLEAERPLA"
     gene            complement(1966..3666)
                     /locus_tag="AC16G0_02660"
     CDS             complement(1966..3666)
                     /locus_tag="AC16G0_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098553.1"
                     /translation="MPRSRWPWTVVSGQTITSVLVGSLAAVLVLYPIFYLLQASLNVG
                     DPQARPPQEYGLANYGGLFQYPTILWNTVVVAVLATVAAVAIGFVMGWILSRTNVPGR
                     GVFEQLMALPYYVTPLMGALAWSFLGTPRSGFINQLWRALGGSDYLVDINTSYGIAWV
                     MALFEGSVAFVMIGAVMKSMDPSLEEASQVLGAGKTRTMLRVTLPLVIPGVLGAAIFV
                     FAEMLGSFSVALVLGLPARFYVVTTAMYQMLAQYPPRFPLAAAMGVSLFAVMLVMVYL
                     YRRIVTAGSYATITGKAFRPRPMHVGGLRWVLFGVCALYLFVAVILPILTVLYASLQK
                     LAMGLPSLAGWTLDNYATAMSLDAVRSALWNSLLLGLGTATIGVLLMGLLAWIIYRSR
                     IPGSGIIEYILMFPQAVPRLVFAFGMLWAWLSFPFSIYGTLWLLLIAYLTVFLPLGIR
                     TISSVLLQIDKSLEESAQMCGASWSYRLRTVTLPLLRPGMIAAWLLLFIASVRELGAS
                     ILLTGPNAKVITPAIVEAWFSISTELTAAMALIQTLAVAVALALLFTFARRASQYGGE
                     "
     gene            complement(3851..>4389)
                     /locus_tag="AC16G0_02665"
     CDS             complement(3851..>4389)
                     /locus_tag="AC16G0_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098554.1"
                     /translation="EYKDIISAKLSSSGTQHAQWYMLRKLYGDDFWKEFAKLKPRGFD
                     SRAQLFDRLSKGDDAVCALAEYAGYVHVKDKGADIEFVAPPDGLPATPIVLGIPTKAP
                     HPEAAKLFIDWALSLRGQAVYQNNKILLYGSVRSDAPAMVTGKRLSDFKLLFPSDWAD
                     FFASHGTFVKEWNAIMGL"
BASE COUNT          829 a         1451 c         1422 g          687 t
ORIGIN      
        1 ctcgctggcc tacgtggagc cgctccacct ataatgcaac gtaccccatt acccatcaag
       61 atggtcacta cctcaggtcc tatggaaagg aaccactgat gaaggatctc ctcccgctgg
      121 cggaaaaggt atccgcgttg ctcaaatctc ggcgcgagac ggtggcggtt gccgagtcgt
      181 ctgccggtgg actcatctcg gcagggctgc tcgccgtgcc aggtgcctcc acgtacttcc
      241 tcggcggcgc agtcgtctat acgtacgggt cgcggggcgt attcctagat gttcccgagg
      301 cggcgctcag ggacatccgg ccggcgactg aagcatatgc cctgctcctc gcacgctccg
      361 tacgccagcg tctcgcggcc acgtggggcc tggcggagac gggtgcgacg gggccgacgg
      421 gcaaccggta tggggatcca gccggccatg cttgcctcgc ggtcaccggt ccggcagagc
      481 gtgcgattac gctggagacg ggccgggccg accggctcgc caacatgcac gcgtttgcga
      541 cagcgaccct ggatctcttc gcgcaactcc tagcgagata actccggccg ctcccccgct
      601 ccggtcgtgc ttctccgcat actgggggat tctgcgttca aactactatt ctctgccggc
      661 gccctctctc gggcagtgcc tcaccgggta gggcggtccc acccggctgc attgctgcgg
      721 cacctcgatt gctctgccga gcgggaacca ccgggaagtc cacggctcca atactcaagt
      781 gagcgatctc aggcgagggg tcgctccgct tccagcaaga cacagcggcg cggatcgacg
      841 gtaaggtagg cctggtcccc ctcttcgagc aattccgtgg ccgcacagcg gacgaccagc
      901 tggcgattct cccagtccac atggtactgg gtgaaatccc cctggaacac acgtcggcgg
      961 atgcgtacag accatacgtt ggcctgggcc gccggcgcct cgcggctgag tcccaggtga
     1021 atggttcgca ccgagaagac gccgtcaggg ccgaccggcc ggccgtgcgc cgtcccgtac
     1081 acgatggtgc ctccggatgt ctccagcgcg atcagcccat cgcggccaca ctcgggccgg
     1141 tgacggcccc ggatcaggtt ggccgacccg acgaagtcgg ccacgaaggc gttgcggggg
     1201 aagttgtaga tctcggacgg cgtgccgatc tgttcgatgg ccccgtctcg catcacggcg
     1261 atccgatccg aaatcgccag cgcttcctcg agatcgtgcg tgacgtagac cgacgtgatc
     1321 cccacccggt gctgcagttc ccggagttcg atgcgcatct cggcgcggag cttggcgtcc
     1381 aggttggaga gaggctcgtc gaacaggagc accgatggcg agaacacaaa ggcacgcgcc
     1441 agggcaaccc gctgctgctg accaccggag agcttggatg cgctgcgctc tgcgaaacct
     1501 cccatctgca ccatgtccag ggcccagcgg accttttcgg cgatctctcg cgtgcccaac
     1561 cggcgaaccc gcagaccgta ggccacgttg tcgaagacgg tcatgtgcgg ccagatggcg
     1621 tacgattgaa agaccatcga caggccgcgg cgctcggccg ggacgttgat ccgttctgcg
     1681 gaggagaaga cggccgctcc cccgatcacg atcttacccg aggtcggcgg ttccaggccg
     1741 gcgatggcgc gcagcgtggt ggtctttccg cacccggagg gccccaggag ggtcacctgc
     1801 tcgccgggcc ggacggtgaa gctcaccccg cgcaccgccg gcacatgccc gtactggacg
     1861 acaaggtccc tgacctcgat atgggcctcg cgggggccac cgggggacgt cgccgatgtc
     1921 gtcggcgagt cgctgtcgtg gatcactcca ccctcgtggg tgccgtcact cccctccgta
     1981 ctgggaagcg cgacgtgcaa aggtgaacag gagggcgagg gcaacggcga cggccagcgt
     2041 ctggatcaga gccatcgccg cggtgagttc cgtgctgatg gaaaaccagg cttccacgat
     2101 ggccggcgtg atcaccttgg cgttcggccc cgtcaagagg atggatgccc cgagttcccg
     2161 gacgctggca atgaacagga gcagccaggc ggcgatcatc cccggcctga gcaggggcag
     2221 cgtcacggtg cgcaggcgat aactccagga cgcaccacac atctgtgccg actcctccag
     2281 gctcttgtcg atctgcagca gcacgctgga gatcgtgcgg atgcccaggg ggaggaacac
     2341 cgtcaggtag gcgatcagga gcagccagag ggtcccgtag atggaaaagg ggaagctcag
     2401 ccaggcccac agcatcccga aggcgaacac gagccggggg accgcctgcg ggaacatcag
     2461 gatgtactcg atgatgccgc tgccggggat gcgcgagcgg tagatgatcc aggccaagag
     2521 gcccatgagc agcacgccga tggtggcggt acccagcccg aggagcaagc tattccacag
     2581 ggcggagcgc accgcatcca gggacatcgc ggtggcgtaa ttgtccagcg tccagcccgc
     2641 cagcgacggg agccccatcg ccagcttctg cagcgaggcg tacaggacgg tcaaaatggg
     2701 gaggatcacg gcgacaaaca ggtagagggc gcagacgccg aacagcaccc agcgcaatcc
     2761 tccgacgtgc atggggcgcg gccgaaaggc cttcccggtg atcgtggcgt agctgccggc
     2821 cgtcacgatg cggcggtaga ggtagaccat gaccaacatc acggcgaaca gggacacccc
     2881 catggccgcc gccagcggaa accgcggggg gtattgcgcg agcatctggt acatcgccgt
     2941 cgtcaccacg tagaaacggg ccggaagccc gagcacgagc gccaccgaaa aggacccgag
     3001 catctccgcg aacacgaaga tggccgctcc cagaaccccg gggatcacca ggggcagggt
     3061 cacgcgcagc atggtccggg tctttccggc cccgaggacc tgcgacgcct cctccagcga
     3121 gggatccatc gacttcatca ccgcgccgat catgacgaat gcgacggacc cctcgaagag
     3181 cgccatgacc caggcgatac cgtaggaggt gttgatgtcg accaggtaat ccgagccccc
     3241 gagggcacgc cagagttggt tgatgaatcc gctccgcggt gtcccgagaa agctccaggc
     3301 gagcgcgccc atcagcggcg tcacgtagta gggaagcgcc atcaactgct cgaacacccc
     3361 ccgcccaggc acattcgtcc ggctcaggat ccaccccatc acgaagccga tggccaccgc
     3421 cgccaccgtg gccagcacgg ccaccacaac ggtgttccag agaatggtcg gatactggaa
     3481 cagcccgccg tagttggcga ggccgtattc ttggggaggt cgcgcctgcg gatcgccgac
     3541 gttcagcgaa gcctggagca agtagaagat agggtacagc accaggaccg ccgccaggct
     3601 gccgaccagc acgctggtga tagtttgccc cgagacaacg gtccatggcc accgtgaacg
     3661 cggcacgtcc tcgctcctag ccggccgctg aaaaaggtcc atctgcgggg acccacctgc
     3721 ggtgggtacc cccgcgctcg cgcggcgctg cgacgtacgg ataaagtacg cctcgcacct
     3781 cgccttgcgc gccgccttgc atctggaccc ttttgagcag ccggcgcgaa aacgggtcct
     3841 tcagcatgac ctagagaccc atgatcgcgt tccactcctt cacgaaggtc ccgtggctcg
     3901 cgaagaagtc ggcccaatcg gacgggaaca acagcttgaa atcgctgagc cgcttcccgg
     3961 tcaccatggc cggcgcgtcc gaccgaaccg agccgtagag cagaatcttg ttgttctgat
     4021 acaccgcctg cccccgcagg gacaaggccc agtcgatgaa cagcttcgcc gcttcgggat
     4081 gcggggcctt cgtcgggatt cccaggacga tgggcgtcgc cgggagtccg tcaggcggcg
     4141 cgacgaactc gatgtcggcc cccttatcct tgacgtggac gtacccggcg tactcggcca
     4201 gggcgcagac cgcgtcgtcg cccttggaga gtcggtcaaa gagctgggcc cgcgaatcga
     4261 agcctcgcgg cttcagcttg gcaaactcct tccagaagtc gtcgccatag agcttgcgaa
     4321 gcatgtacca ttgggcatgc tgggttccgg aggacgaaag cttggcgctg atgatgtcct
     4381 tgtactccg
//
LOCUS       DBNGZC010000139         1928 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_500031, whole genome shotgun sequence.
ACCESSION   DBNGZC010000139 DBNGZC010000000
VERSION     DBNGZC010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_500031"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..921
                     /locus_tag="AC16G0_02670"
     CDS             <1..921
                     /locus_tag="AC16G0_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR5098555.1"
                     /translation="PAPGPGFGARTTRERFRRSRLYLPGNEPRYFLNASQHKPDAIIL
                     DLEDSVAPPAKDAARLLVRNALRAVDFGECERMVRINQGKRGLEDVDAVVPQNVHVLL
                     IPKVETAEQVQAVDARADKVRMAAGLESPCLLMPIIESALGCFNALAIATASPNVVAL
                     TIGLEDYTADLGAQRTPEWRESFWARQVVVNAARAAGVTPIDTVFSDFGDMEGLRQAC
                     LEAKSLGFEGKGCIHPRQVPVVHEAFAPSSEEVEKAKRIVLAFEDAERKGLGVVALGS
                     KMVDPPVVKRALRTVRMAEALALLSKDWRG"
     gene            1121..>1928
                     /locus_tag="AC16G0_02675"
     CDS             1121..>1928
                     /locus_tag="AC16G0_02675"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR5098556.1"
                     /translation="MEHKRNIILTGFMATGKSSVGRQLALRLGYEFLDLDALIEAEAG
                     MPIARIFATQGEAAFRSLESRMAERIAGRPGCVVATGGGTIANPRNLAALQQSGVVVT
                     LTADPDTILSRIGPEDDRPMLQGRDKRERIRVLMEQRASAYAKADLIVDTSSRTVEEV
                     AEYIAEVLPLRPPETRELATTALPPGFQSETVRVNLGNRGYDIQVGRDLLRHVGRLVK
                     PLGLGRCLGLVTHPVLMETHQYAANVADSLREAGYEVRVIAVPSGEESKSL"
BASE COUNT          342 a          609 c          650 g          327 t
ORIGIN      
        1 cctgcgccgg ggccgggatt cggcgcgcgc accacccgcg agcgcttccg ccgctcacgc
       61 ctctatctcc cgggcaacga gcccaggtac ttcctgaatg ccagccagca caaaccggat
      121 gccatcatcc tggacctgga agattccgtg gctccgccgg cgaaggacgc agcccgcctt
      181 ctggtccgga acgccctccg ggccgtggac ttcggcgaat gcgagcgcat ggtgcggatc
      241 aaccagggaa agcgaggcct ggaagacgtg gacgccgtgg tgccgcagaa cgtgcacgtt
      301 ctcctcattc ccaaggtcga aacggcggaa caggtgcagg cggtggacgc ccgcgcggac
      361 aaggttcgca tggccgcggg cctcgaaagc ccgtgtctcc tcatgcccat catcgagagc
      421 gcgctggggt gcttcaacgc cctggccatc gcgacggcca gcccgaacgt cgtcgcgctc
      481 accatcgggc tcgaagacta cacggcggat ctgggcgccc agcgaacccc ggagtggcgc
      541 gaatccttct gggcgcgaca ggtggtcgtc aacgccgccc gggctgcggg cgtgaccccg
      601 atcgacacgg tcttctccga tttcggcgac atggagggcc tccggcaggc atgcctggag
      661 gccaagtccc ttggattcga gggcaagggc tgcatccacc cgcggcaggt gcccgtggtg
      721 cacgaggcct ttgcgccatc gtctgaagag gtcgagaagg ccaagcgaat cgtcctcgcc
      781 ttcgaggatg ccgagaggaa ggggcttggg gtggtggcgc ttggctccaa gatggtggat
      841 ccgccggtcg tgaagcgggc cctccgcacg gtgcgcatgg ccgaggccct ggcccttctc
      901 tcaaaggatt ggcgaggcta acaggctgcc gaaaaaggcg ccgtctgccg ggtacccaag
      961 tctttccctc ccttcagggt cacctgccag gacgatccag ccgccagtat gcgccagcag
     1021 gttcgatgct gtgcgggcac ttcgcccttg actggtcggg cagggtgccg taaggtcgtc
     1081 gtcgcctcac tgatgccaat cgatgcagtc gatcgcgatc atggaacaca aacggaacat
     1141 cattctcaca ggcttcatgg cgacggggaa atcctcggtc gggagacagc ttgccctgcg
     1201 cctgggctat gagtttctgg accttgacgc cttgatcgag gccgaggcgg ggatgcccat
     1261 cgctcggatt tttgccacgc aaggagaagc ggcattccgt tcgctcgaat cccggatggc
     1321 ggagcggata gccggccggc cggggtgcgt ggttgccact gggggcggca ccattgcaaa
     1381 tccgcgaaac ctggcggctt tgcagcaaag cggcgtggtc gtgaccctca ccgcggatcc
     1441 ggataccatc ctgtcccgga tcggaccgga ggatgatcga cccatgctcc agggcaggga
     1501 caagcgcgag cggatccgtg tgctgatgga gcagcgggcc agcgcctatg ccaaggcgga
     1561 tctgatcgtg gacacctcgt cccgcacggt ggaggaggtg gcggagtaca tcgcggaggt
     1621 cctgcctctg cgcccacccg agacgcggga attggctact accgcactcc cgcccggctt
     1681 tcagagcgag acagtccgcg tcaatcttgg gaatcgggga tacgacatac aggtcggcag
     1741 ggaccttctc cgacatgtgg gaaggctggt gaaacctctg ggcctcggcc ggtgtctggg
     1801 actcgtgacc cacccggtac tcatggagac ccaccagtat gcggcgaatg tcgccgactc
     1861 gttgcgagag gccggatacg aggtcagggt gatcgccgtt ccttcgggag aggagtctaa
     1921 atcccttg
//
LOCUS       DBNGZC010000140         6090 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1870365, whole genome shotgun sequence.
ACCESSION   DBNGZC010000140 DBNGZC010000000
VERSION     DBNGZC010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1870365"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="AC16G0_02680"
     CDS             complement(<1..394)
                     /locus_tag="AC16G0_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098557.1"
                     /translation="MTRRLVLMLGLGVSCSLLGCATIPPISEVSPKKLIEFGWDEPNT
                     AFMRQHITEMERSPFDGCVFHVTYTKPDGRSGEFTWEGWGTRAFTQAEVQSALDDLQA
                     IHFRRFTHNFLRFNTVPGDVDWFDDFSAI"
     gene            608..1360
                     /locus_tag="AC16G0_02685"
     CDS             608..1360
                     /locus_tag="AC16G0_02685"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014281775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR5098558.1"
                     /translation="MKEKLLTGQPAVGVSVMFPSPQIVEMVGRLGFDWVLLDCEHGSL
                     SPESVELMAMAAEAVGITPIARPTLNSPEAILRVMDRGVLGVQIPHVNTAAEARHAVE
                     AVKFHPLGNRGLAAGTRPAGYGFGLSSAAYVQEANRETLVCVQLEETEALHNLDDILR
                     VEGVDVFFVGPSDLSQSMGYPGRSDAPEVQAAMDKAFAAIMAAGKVPGSTGNAQAIRR
                     HLQHGVRYLYTHVPTLLASGSRDFLAAVKGGV"
     gene            1361..1759
                     /locus_tag="AC16G0_02690"
     CDS             1361..1759
                     /locus_tag="AC16G0_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR5098559.1"
                     /translation="MDRQRVASGTPWEPIVGYSRAVRVGSAVYVSGTTATDAAGRIVG
                     LGDPYAQAVQALKNIEVALARAGAGLKHVVRTRIYVTNIDDWEKIGRAHGEFFRDIRP
                     ATSMVEVKRLIAPEILVEIEADAVITQPGD"
     gene            1822..2385
                     /locus_tag="AC16G0_02695"
     CDS             1822..2385
                     /locus_tag="AC16G0_02695"
                     /EC_number="3.5.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004132 - dCMP deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006220 - pyrimidine nucleotide metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009972 - obsolete cytidine deamination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxycytidylate deaminase"
                     /protein_id="HIR5098560.1"
                     /translation="MNAADRLRPDRDRYFMGIAMAVRTRANCRGNHVGAILVLDGHVV
                     STGYNGTPSDMLNCEEGGCDRCANREKYGAGKGYDLCICVHAEQNALLSAARFGIPVQ
                     GAILYTTMRPCFGCTKELLQAKVKAVYYLHDWKHPDPDVWHEYEKLQQRFPGGIRKLE
                     LDDPDKNWAVSTKAPVPEESGHSIPGN"
     gene            complement(2741..3814)
                     /locus_tag="AC16G0_02700"
                     /pseudo
     CDS             complement(2741..3814)
                     /locus_tag="AC16G0_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697868.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
     gene            complement(4081..5136)
                     /gene="selD"
                     /locus_tag="AC16G0_02705"
     CDS             complement(4081..5136)
                     /gene="selD"
                     /locus_tag="AC16G0_02705"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5086..5088),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HIR5098561.1"
                     /translation="MSGKEIRLTEYAACAGUASKLGPGDLHEVLGALTKQQHPDLLVG
                     LDRSDDAAVYRLNERQAIIQTVDFFPPIVDDPYTYGAVAAANSMSDVYAMGGQVLLAL
                     NVAGFPREFPHSAISKIFQGGADKVAEAGAVIAGGHTVVDKEPKYGLCVTGIVHPDRV
                     TIKGGLVPGHRLFLSKPLGTGVIATAGKAERTKAAHMEAAIQSMTRLNRGAAEVLSEI
                     GIAGCTDITGFGLLGHGSEMVLASRCGVRIRAAAVPLLPGALDYAGGGHFAGGLGRNR
                     TYVEGLAQEGRIALRIASALSAEHVGLLYDSETSGGLLFSAAADQAHEVRDRFARKRE
                     AVWEIGEVIPEVAIEVM"
     gene            complement(5306..6037)
                     /locus_tag="AC16G0_02710"
     CDS             complement(5306..6037)
                     /locus_tag="AC16G0_02710"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR5098562.1"
                     /translation="MRWSTLDRWRIGPCLIFAAIVLWLIAGTGQAWGQAPPEIGWLGI
                     SITDVGEELADRLATTFGPDAGTGVLVADVLKGGPAEQAGLHRGDVIVKLDTQPIWDV
                     RQLQRTVRSRPVNQRVTIIVLREASRVGLPVIIGSMPAPARAQLAGEPFGFLVREEDL
                     GEARRGTSSPVSRIFVVFVDSGSPAAQAGLSPQDVILQVNNLSVQGLEDFDRAMRRVK
                     GSVSLQVERRGTSSPLTLTLEHPRR"
BASE COUNT         1159 a         1976 c         1952 g         1003 t
ORIGIN      
        1 ggatcgccga gaaatcgtcg aaccagtcca cgtctcccgg cacggtgttg aagcgcagga
       61 agttgtgggt gaatcgacga aaatgaatgg cctggagatc gtcgagcgct gattggactt
      121 cggcctgggt gaaggcccgt gtgccccagc cttcccacgt gaactcgccg gatctgccgt
      181 cgggcttggt gtaggtgacg tgaaaaacgc agccgtcaaa ggggctacgc tccatctcgg
      241 tgatgtgctg gcgcatgaac gcagtattcg gctcgtccca gccgaactcg atcagctttt
      301 tcggcgagac ttccgatatc ggagggattg tcgcacagcc gaggagcgag caactcacgc
      361 cgagaccgag catgaggacc agtcgacgtg tcatgattcc cctcgtcctt gcttcgccgg
      421 gacaggatag aagacccacc cgaacgggtg tctgtttggg attataccaa aaaaccagtt
      481 tgatcttgac acctcctcgt ggagcgcgtt agcgtcggcc accttgcggc gccccccgca
      541 aacggtctgg gcggaagatg tcggcttcgg agacgccccc gacaggtagg ttgtcatgaa
      601 gaaccggatg aaggagaaac tgctgacagg ccagccggcg gtgggggtgt cggtgatgtt
      661 cccatccccg cagatcgtgg agatggtggg ccggctggga ttcgactggg tcctcctcga
      721 ctgcgagcat gggtccctct ctcccgaaag cgtggaactg atggctatgg cagcagaggc
      781 ggttggcatc acgcccatcg cccgccccac gttgaacagc cccgaggcca tcctgcgggt
      841 gatggatcgc ggggtgctgg gggtgcagat cccgcacgtc aacaccgctg ccgaggcccg
      901 gcatgccgtc gaggcggtca agttccatcc cctgggcaac cggggcctgg ccgccgggac
      961 ccgtcccgcc ggctacgggt tcggcctatc gtcggccgcc tacgtgcaag aggccaatcg
     1021 cgagaccctg gtatgtgtcc agctggagga gaccgaggca ttgcacaacc tggacgacat
     1081 cctccgggtg gagggggtgg acgttttctt cgtcggcccc tcggaccttt cacaatccat
     1141 ggggtatccg ggtcggtcgg atgcgcctga ggtgcaggcg gccatggaca aagcgttcgc
     1201 cgccatcatg gccgctggga aggtgccggg gtcaaccgga aatgcccagg cgatccgccg
     1261 gcacctccag catggcgttc gctacctgta cacgcatgtg ccgacgcttc tggcaagcgg
     1321 cagccgggat ttcctggcgg ctgtcaaagg cggcgtttga atggaccggc agcgcgtcgc
     1381 cagcggcacg ccctgggagc ccatcgttgg ctactcgcgg gcggtccggg tgggttccgc
     1441 cgtctatgtc tccgggacca ccgccaccga cgccgccggg aggatcgtgg gactcgggga
     1501 tccctacgcg caagcggtcc aggccctcaa gaatatcgag gtggcccttg ctagggccgg
     1561 cgccggcctg aagcatgtcg tgcgcacccg gatctacgtc accaacatcg atgactggga
     1621 aaagatcggt cgggcccacg gcgagttctt ccgggacatc cgcccggcca cgagcatggt
     1681 ggaggtgaag cgcctgatcg cccccgagat cctggtcgag atcgaagcgg acgcggtgat
     1741 tacccagccg ggagactgac cgcggcggcg tccgagactg ccgaggagag ggattccgct
     1801 cccgtccacc tggaggtgcg catgaacgcc gcagacaggt tacgtccaga ccgcgaccgg
     1861 tacttcatgg gtatagccat ggccgttcgg acccgcgcca actgtcgcgg caaccacgtg
     1921 ggagcgatcc tggtgctgga cggccatgtc gtctccacgg gctacaacgg gacgccctcg
     1981 gatatgctaa attgcgagga gggcggctgc gatcgctgcg ccaatcgcga gaagtatggg
     2041 gcgggcaagg gctacgatct ctgcatctgc gtgcacgccg agcagaatgc cctcctgtcg
     2101 gcggcgcgct ttggcatccc ggtccagggg gcaatcctct acaccacgat gcgaccctgc
     2161 ttcggctgca ccaaggagct tctgcaggcg aaggtcaagg cggtctatta tctgcacgac
     2221 tggaagcacc ccgaccccga cgtgtggcac gagtacgaga agctccagca gcgcttcccg
     2281 ggcgggatcc ggaagctgga gctggacgac cccgacaaga actgggcggt gtccacaaag
     2341 gcccccgtcc ccgaggagag cggccactcg attcccggaa attgatgacc tgcgtccgtc
     2401 gccgtgcccc atcgctgcct gagccatgca atcgctcctc ccccctcatt cgactcttca
     2461 ggtgccaacc tggagatctg ctgcaaaagc ggcacggggg acaatctacc ttaccggccg
     2521 tctatgcaac aaaacggaaa tgtcgatcgt cggtcgtatg ggaactgccg cctcggacga
     2581 cctcatgacg ttccagtgtg catggcattc tggtgggtgc cttctcagga tggggtcgcc
     2641 gccacgtgag tgaacagaca gttggcgggg aatactgcgt ccgcgaggaa gaacaggcgg
     2701 tggggtccgg ggccccgcgg ccggggtccc ggcaagagac ctagaatcca agtcccacgg
     2761 cgaccgctgc gctcagcacg ccgaggagca ccacagacgc agcgatgaga accagcaccc
     2821 gcaccgggat cgacttgccc agcatcacga gcgacgggag actcacgggc ggaagtgtcg
     2881 tgaggagtgc cgcggcaggt cctacgccca ggccgaatgc catcagggtc tggacgatgg
     2941 gaatctctcc ggcggtgggg atgacgaaca gggtgccggc aatggccagt cccaccgtcc
     3001 agagaacgcc gctcgtcgct ggagtcactg ccgggaacag ccaggctcgg gctgcgccga
     3061 ggatcaggac aattcccaca tattccggca cgagcccaat gaccatttgc cagagcacgc
     3121 gcagccaacg gaaccacagg gaaccctcag cgttgccagt ccccaccgcc ctcgcaggtg
     3181 gagccacggc ccggagatcc tccggacgga cgaatcggtt ggcgaggtag gcgacgccaa
     3241 ataccagaag cagaccgtcg aaaagtcgta gtgccgtcca ctgccatacc aggacgaacc
     3301 ccatgaagac catggacgcc gggttgagga tcgggttgcc catccagtag gcgagcgtcg
     3361 ggcctgcggc cgagccgctc cgtgcgagcc ccagtgccac tggggcggcg cagcacgttc
     3421 acatcatgga cgggactgcg gccagaccag ccaccgcaac accggagaag ttcatccgcc
     3481 cgaggaaccg cgccagccag tcccgcggaa cgagcacttg caccccggcc cccagaacca
     3541 aacccaggac catggcttgc cagatcgcct tcccgtaggc ccagccgtat cccagggctg
     3601 ccgccaagga tggcggcggt gctgtggccg aattgccgga gacgatagaa gcgccgatgg
     3661 aatgctgcgc cgccgccacg aagccccggt ggaagtacgg attccacttc acataataga
     3721 ggccggccac ggccacgccc gtaaggattg tcactcctac caggtatcgc cgctccacag
     3781 gacccgaata actggcagac actccgttgc tcatgtcgcg ctccctgtct catgcattcc
     3841 agacctagat atcccttccc ttggcgattg accacgcctc ggcggtcggt ttctaggcgg
     3901 ccgagggatc gaaaagcata gcggagtgta acggtgtagt atgaaaggtc aaatcggaat
     3961 ttacgatgga agatgcttca aggataggta gatcaggggg aagctctggg tgccgttctc
     4021 tggctgcgag actcgaatct tcgaccaggc gcgtggtcgt cggttgtgga ttgggagcag
     4081 ctacatcacc tcgatggcca cctcggggat gacttccccg atctcccaca cggcctcgcg
     4141 cttccgcgcg aaccggtcac gcacctcgtg ggcctggtcc gctgcggcgg agaagagcag
     4201 gccgccagag gtctccgagt cgtacaggag cccgacgtgc tcggcggata aggcggacgc
     4261 aatccggagg gcgatcctgc cttcttgggc caacccctcc acatacgtcc ggttccgtcc
     4321 caggccgccg gcaaagtgcc cacccccggc gtagtccagg gcaccgggaa gcaagggcac
     4381 cgccgcggcg cggatacgaa ccccacagcg gctcgccagg accatctcgc taccgtgacc
     4441 cagaaggccg aagccggtga tgtcggtgca acctgcgatc ccgatctccg agagcacctc
     4501 ggcggctccc cggttcagtc gggtcatgct ttggattgcc gcttccatat gggcggcctt
     4561 ggtccgttcg gccttgcctg ccgtggcgat gacaccggtg cccaagggct tgctgaggaa
     4621 cagccggtgt cccggcacga gaccgccctt gatcgtcaca cggtcggggt gcacgatccc
     4681 ggtgacgcac aggccgtact tcggctcctt gtccacgaca gtgtgcccgc cggcgatgac
     4741 cgccccggct tcggccacct tgtccgcccc accctggaag atcttgctga tggcgctatg
     4801 aggaaactcc cgggggaaac cggcgacgtt cagggccagc agcacctgcc cgcccatggc
     4861 atagacgtcg ctcatggagt tggcggcggc caccgcgccg taggtgtagg ggtcgtccac
     4921 gatgggcgga aagaaatcca ccgtttggat gatcgcctga cgttcgttca ggcgatagac
     4981 ggctgcgtcg tcactccggt ccaggcccac cagcagatcc ggatgctgct gcttggtcaa
     5041 agcccccaag acttcgtgaa ggtcccccgg acctagcttg gaagctcacc cggcgcaggc
     5101 cgcgtactcg gtgagccgga tctctttccc actcatgccg cgcctccgtc cgccgtcgcc
     5161 gatatcgcct cgcaccttcg caaggcatca tggataccgc actggcgggg atgtgaaacc
     5221 tcggttccgc cgagaagaaa tccgagggtc tcgacgggcg ccgggattga tgacccccgg
     5281 caggctacaa atgaccagcg gaaacctatc gccgcggatg ttccagggtc agcgtgagcg
     5341 gactcgaggt gccgcgccgt tcgacctgaa gagagaccga tcccttcacg cgtcgcatgg
     5401 cgcggtcgaa gtcctccaga ccctggaccg atagattgtt gacttgcagg atgacatcct
     5461 gaggactgag cccggcctgc gcggcgggcg agcccgagtc cacgaacaca acgaaaatcc
     5521 ggctaaccgg gctcgatgtt ccgcggcggg cctcgccgag gtcctcctcc cgcaccagga
     5581 acccgaaggg ctccccggcg agttgggccc gggccggagc gggcatcgag ccgatgatga
     5641 ctggcaggcc gacccgggag gcttcccgca agacgatgat cgtcacgcgc tgattgacgg
     5701 gccgggatcg aacggtccgc tggagttgtc gcacatccca gatcggctgg gtatccaact
     5761 tgacgatgac atcgccccgg tggagaccgg cctgctccgc agggccccct ttcagcacat
     5821 ccgccaccag cacgcccgtg cctgcatccg gcccgaaggt cgtggccaat cgatcggcca
     5881 gttcttcccc aacatcggtg atggagatgc cgagccagcc gatctccgga ggggcctggc
     5941 cccaggcttg gccggtcccc gcgatcagcc acaggacaat tgccgcaaaa atcagacagg
     6001 ggccgatccg ccacctatcg agagtggacc agcgcattac ttctggagtt ccttcacatc
     6061 cacgttgaca ccacagacct cgcagtacac
//
LOCUS       DBNGZC010000141        13057 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1283828, whole genome shotgun sequence.
ACCESSION   DBNGZC010000141 DBNGZC010000000
VERSION     DBNGZC010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 13057)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13057)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13057
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1283828"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="AC16G0_02715"
     CDS             complement(<1..219)
                     /locus_tag="AC16G0_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase YdiF"
                     /protein_id="HIR5098563.1"
                     /translation="MGDKVITARAAIDLVKSGDTLAIHGAGGGNVEPDLLIRTLAEKF
                     GETGQPRDLTVFHVSGLGNWTDSGLNQLT"
     gene            complement(266..1039)
                     /locus_tag="AC16G0_02720"
     CDS             complement(266..1039)
                     /locus_tag="AC16G0_02720"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5098564.1"
                     /translation="MDLGLKDRVAIVTGGSKGLGRAEAEALAAEGAAVVVATAKSMAE
                     ADRVVRGLLDKGGRAISVQADVTRTADVQRLVETALKTFGRLDVLVNNAGTTGPQYNK
                     PLVDMPEDLWDFMVGNHLRSVFLCIKFAAPHMVKQGWGRIINTSSIHGRVGGRPSLGH
                     YGAAKAGVIALTMTAARELGPKGITANVIAPGFIGTETLRGILAPPTLEKLQQQIPLG
                     RLGEPLEVGRVVAFLASEAASYVNGAVVDIGGGRLEFFL"
     gene            complement(1077..2102)
                     /locus_tag="AC16G0_02725"
     CDS             complement(1077..2102)
                     /locus_tag="AC16G0_02725"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5098565.1"
                     /translation="MDKLRVLVLGVGFFGKNWLREVSACPESEVAGVVAKHADLLSSV
                     GDEFKIPTSRRFETIEAGLDRAGAEAVIVALPEMAHRVAIVSALERGLHVLTEKPLAL
                     TMAEAGVVVRAARTARNSVLMVDQNYRWRPQTQALRRAIREGRIGPPASLTHEFRQPI
                     TRTTTDGWREQMPHPYLHDMAIHHFDLLRASTGLECKELLAVGVRPPWSWVRGVPGVD
                     AVLSFERNLSVTYTGTMVGRGLATPQDGIITAMGEEGMLRLEADSQVRWYRDKQVETI
                     PLPQMPFTDTGYALQEFLAAIRERRQPETHVEDNVRSLAMVAAAITSVETHRLVAVQP
                     LVEEALG"
     gene            complement(2169..2936)
                     /locus_tag="AC16G0_02730"
     CDS             complement(2169..2936)
                     /locus_tag="AC16G0_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5098566.1"
                     /translation="MASQLFDLTGRVALITGSYQGLGLGIARGLGQSGATLILNGRNE
                     EKLNKAVTALSGEGLKVFGSCFDISNTAQVKQKIDALEREVGPIHILVNNAGIQRRSP
                     LEQFEESVWREVLDTNLTGLFLVTKQVVHGMIARKAGKIINICSLMCEVGRPTIGAYT
                     AAKGGVKMLTKAMAVEWGKYNIQANGIGPGYFTTEMNRPLYEDPKFDAWIRGRTPMGR
                     WGDPSELVGAAVFLASRASDFVCGQVIYVDGGILAAL"
     gene            complement(3003..3842)
                     /locus_tag="AC16G0_02735"
     CDS             complement(3003..3842)
                     /locus_tag="AC16G0_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003572412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HIR5098567.1"
                     /translation="MPDLIRQYDNKNQPIITSGKTILSLTYFNLVRLRKGEEYQAKVE
                     GFETLYAVLSGNVDVDVNGAAFRDVGQRKDIWSGKADSVYAGAGAGVRVRANKDGTEV
                     AVAGGICDKTFPPFHVPPEEVEMVNVGSIETRSHRRIFHLLGQNGNGRAGNLLISELY
                     CDHGCWSGYPPHKHDEDRGSGETAHEETYHYRFRPETGFGAQLCFQPDGSAKCFMTRN
                     GDTFLLDRGYHPTVTSPGHEEYIFTILVGKSRRGLVQNFKEDYHYLMAGIPGIQDMVN
                     KFK"
     gene            complement(3899..5191)
                     /locus_tag="AC16G0_02740"
     CDS             complement(3899..5191)
                     /locus_tag="AC16G0_02740"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR5098568.1"
                     /translation="MLIGLFVALLLLLFGGAPVLLGIGIAGLLGMLASPGVVLAIFPQ
                     KMFAMLDSFSLLAMPFFILAGELMCQGGISKKMVAFSETVVGHLRGGLGHASVVGSMI
                     FAGVSGSSVADTSAIGSILIPSMKDRGYKPGFAASLLAAAGTIGPIIPPSMTMIIYGS
                     MTGTSIGGLFLSGIIPGILIGIGLMGTIYVHALLPQFPELRITTGRFSLIAMLKALGQ
                     VWSALLAPVIILGGILSGVFTATEAGVVACWYAFIVSFIVYKSLRLRDLPKILVSAAI
                     TTTTVVGIISVAGAFGWLLSYLDFNVIVLDLLKSISNDRMVILLVLLAVIFIMGMFIE
                     SLAVLIILIPVCTYVTKAFGFDPFHFGLLMTIATQIGAVTPPVAVLLFVATSIAGCKY
                     DETIRYCYPFIATLVIVMLLVAFVPITATYIPHRFLGP"
     gene            complement(5185..5685)
                     /locus_tag="AC16G0_02745"
     CDS             complement(5185..5685)
                     /locus_tag="AC16G0_02745"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR5098569.1"
                     /translation="MLDKVLSCVNRAFEAAIFLLFFSIVIVGGLQVFNRFVLNQSLSW
                     SEEFMVYANIWMVFLTIPVAYNHGSHIGMNLFVKGLPPKAQVVMTLAIDLLWLVLAGA
                     IVAYTTVIMRVASNQDSPAMGLRMDRVYLSLAVGWSYMALLALRKVAGSVLLLRSGEA
                     TGGAPC"
     gene            complement(5791..6792)
                     /locus_tag="AC16G0_02750"
     CDS             complement(5791..6792)
                     /locus_tag="AC16G0_02750"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR5098570.1"
                     /translation="MKRPTSLIFLTMGLLVGLVLATTSPALAAKKVIKLSHSHQADFA
                     SEIHTAAWIFQKWIEDNSDTLEVKIYAANALGEERAVYEGMQLGSGATAVISGTAILN
                     NFNKKIAVLDLPFLWKSYDHAHRVLDGKVGEALAKDLDQTGFQVLAWMDSWGFRNVVT
                     AKKEVKTPADLKGLKIRTIQTPTYVAALNAMGANATPMAFGEVYTAMQTGVLDGYEHG
                     SAVTKAQKFYEVSKYIALTQHLFGPLVFAYSKKDWAQLTDKEKAVILEGARMARDVQR
                     TLGPLREQEAFDFLKSKGMVIHKIDTSSFRKNAIAIQDSFAKERGATDLLQMIRATD"
     gene            complement(6914..7660)
                     /locus_tag="AC16G0_02755"
     CDS             complement(6914..7660)
                     /locus_tag="AC16G0_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020534335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR5098571.1"
                     /translation="MADKRQVIEHFRGIDLKTPSEIIIQQIKELISSGVLRPGDRLPA
                     ERALAERFRVGRGHIREAIRKLEFYGILKTIPQSGTMVTSLGAKALEGLISNVLNLEK
                     EDFQSLMETRALLEIQAARLAAERATDAEIADLGRIHEDFCSQAQSGASGLEVDLRFH
                     LRIAECARNSVLRSLISLLTPDIVSLSKSHETPQDGRFVIALQEHEAVLEGIRARDPD
                     RAAAAMEEHIRKVRLQVESYLPWRSPAGPA"
     gene            complement(8033..11722)
                     /locus_tag="AC16G0_02760"
     CDS             complement(8033..11722)
                     /locus_tag="AC16G0_02760"
                     /inference="COORDINATES: protein motif:HMM:NF052799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5098572.1"
                     /translation="MLRRCLDDADDILSGAAAVLIGKRGISALADAILDRLRQGKDMP
                     GAPCAVALAELGDSRVLDYLRARSAQGPLRCDPGVWLALSTLKGAEATRLLREAFEQL
                     PLRGAAPAASLLAGAFVQADPKEALPLVADRWLESPQGEEADALLSALLPAAEFDGDV
                     QELREALNPDRESSWPGLHEALLGMLSELVPLRLTSEASRAGRKGRWVRLIEALAPIA
                     AWLEGEAKRGAAAGPSLALVETLGARSKRLGDALDKAREASALMLLDLVRISRAGRER
                     SVTLPPSLEDRLGWLLSDAADAAPETAGSVFDQLAAEAPQDAWTDACLRTIELKGPQA
                     TTAIDLLAAWHSPAGVAALARLLGDREHPDLAEAAVDALAEIGEAAVDAVVERLGSTE
                     DPVLVEDCLNTCIRLPSRRVVAAICRRFEELWIHAPEPLLRAVEILGAREFIQPLGRE
                     LQEREAAAERAFALLCQLNEVSDPRAEDIRRRLAERVEQEMEAAQDSDREPDASLELP
                     LTCNGCRRTYNYVVREILVDPEATEAEEFQPFILDKIRCKRCGREDDYSLPQRTQLLL
                     MSELALLMERSEQEGVEVLQEGPFRFARMGLSDGRRLHPRDARADYERRLASRPDDPE
                     LLIGYANVLRLLGEAEKSEAALRRALELDPAAADAYATLGQFAEERGDLAAAEEMYRR
                     VVALGRKARFYRVKARGEFLGWIEEALVRVQGVRATHRPEPVSAQARLEALTAQDRGG
                     TKVGRNDPCPCGSGKKYKKCCLPKSERGPDAQAPLGPDGRLRQRLMAYVETSIPHADA
                     HRATREFFGEQFDADRKRLALDSRGVEADWPAFLEWLIHDFPLSSGGSVIARFLKERG
                     HTLPGDERAILEEWQDAAVGLHEVLDLDPGRSLTLRDVFTGETRTVREVRGSLSVARW
                     DLLGARMILVKGEPFLSGTITLFRPRDREGLVSHVTGRYQDHCRDHPGAAWRDFFRAE
                     SLIFHRYAQHLAQEARPPKVHTPEGHPVMLGRLRYEVRDPHRLLTTMAAAPDFEETTG
                     PDEPAGARHFAWIRIGPAERYVKQAALPAEGLMLRTDRLDADGKAGVPGLASLTIDGN
                     DLAVETMSAERLAWAKARLAELAGDAILLRADVIEDPMEKLRTMPEGRRGREASSEIP
                     PEVQTRLLGQTLHRHFTAWLDQEIPALDGRTPRQAARDVLLRPRLIQLLREIENAQDR
                     ERQQGKPWYDAAWMWETLGIRRTEA"
     gene            complement(12045..>13057)
                     /locus_tag="AC16G0_02765"
     CDS             complement(12045..>13057)
                     /locus_tag="AC16G0_02765"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014653.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HIR5098573.1"
                     /translation="DFSREVFACAGFTPEGAELQADVLVWANLRGVDSHGVLRIPWYV
                     ELVDKGLMKPRPNIRVEKETAATLLMDADYAPGPVVTVPAMNRAIQKAREVGIGWVVI
                     RNTLHQGAMGYYPLMAAKQGMAGIAIVCNPPNMAPHGARAIGVHNSPIAVCVPGRRHS
                     HLMLDMATSVAARGKLDLAIDKGASIPLGWALDKDGKPCADPKLATILLPAGGPKGSG
                     LAMMFECLSSLFVNNPLLEPALLGKEGAMRHRQNSVVAAVNIGTFTDVEGYGEHVDGL
                     IDRLKALPPAEGFKEVLIPGEPEDRVQEERSKNGIPLPEGTAQNLRKVAERFGVKLPA
                     GI"
BASE COUNT         2333 a         4405 c         3967 g         2352 t
ORIGIN      
        1 ggtgagctgg ttcaaaccgc tgtccgtcca gttcccaaga cccgagacat gaaacacggt
       61 caggtcgcgg ggctggccgg tctccccgaa cttctccgcc agggtccgga tgagaagatc
      121 cggctccaca ttgccgccgc cggccccgtg gatggccagc gtgtcccccg atttcaccag
      181 atcgatggca gcccgtgcgg tgatgacctt gtcgcccacg cctgcactcc gttccgagtt
      241 atacgtccga ttggtccaat aggtcctata gaaaaaactc gagccgaccc ccaccgatgt
      301 ccacgaccgc accgttcaca taggaggcag cttccgaggc caggaacgcc accacccgcc
      361 ccacttccaa aggctcgccg agacgaccga gaggaatctg ctgctggagt ttctccagcg
      421 tcggcggagc caggattccg cgcagcgttt cggttccgat gaaacccggg gcgatcacgt
      481 tggccgtgat acccttgggt cccagctctc gcgctgccgt catggtgagg gcgatgacgc
      541 ccgccttggc cgcgccgtag tggcccaagc tgggccggcc tccgactcga ccatggatgg
      601 aagaggtgtt gatgattcgc ccccatccct gcttgaccat gtggggcgcc gcgaacttga
      661 tgcagaggaa gacgctccgc aggtggttcc ctaccatgaa gtcccagagg tcctcgggca
      721 tgtccacgag aggcttgttg tactgagggc cggtcgtccc ggcgttgttc accaggacgt
      781 ccagccgccc gaacgtcttc agggcagtct ccaccagccg ctggacgtcc gccgtccggg
      841 tcacgtccgc ctggaccgag attgcccggc cgcccttgtc caggagaccc cggacgaccc
      901 gatccgcttc ggccatgctc ttggcggttg ccacaaccac cgctgccccc tcggccgcta
      961 gggcctcggc ctccgctcga cccaacccct tgctgccgcc cgtgacgatg gccaccctgt
     1021 ccttcaatcc gaggtccatt gcgcccccct tcccagcgtc aatgcacttc ccatccctat
     1081 ccgagagcct cttcgaccag cggctggacg gcgacgagcc gatgggtctc caccgaggtg
     1141 atggccgcag ccaccatggc caacgaccgg acgttgtcct ccacatgggt ctcgggctgg
     1201 cgcctctccc ggatggctgc caggaattcc tgcagggcgt accccgtatc cgtgaagggc
     1261 atctgtggca gcgggatcgt ctccacctgc ttgtcccgat accagcggac ctgggagtcc
     1321 gcctccagac gcagcatacc ctcctcaccc atggccgtga tgatcccgtc ctgcggcgtg
     1381 gcgaggccgc gcccgaccat cgtgcccgtg taggtcacgc tgaggttccg ctcaaagctg
     1441 agaacggcat ccactcccgg cactccgcga acccagctcc acggcggcct gacgccaacg
     1501 gccagcagtt ccttacactc cagaccggtg gatgcccgca ggaggtcgaa gtggtggatg
     1561 gccatgtcgt gcaggtaggg atggggcatc tgctcgcgcc agccatccgt ggtggtccgg
     1621 gtgatcggct ggcggaactc atgcgtgagc gacgcggggg gcccgatcct cccctcgcgg
     1681 attgcccggc ggagcgcctg ggtctggggc cgccagcgat agttctggtc caccatgaga
     1741 acagagttcc tggcggtgcg ggcggcccgc acgacgacgc ctgcctcggc catggtcagt
     1801 gccagaggct tttccgtgag gacgtgcagg ccgcgctcca gggcggatac gatcgccacc
     1861 ctgtgggcca tctccggcag ggccacgatc accgcctcag ctccggcgcg atccagtcca
     1921 gcctcaatcg tctcgaaacg ccgagacgtc gggatcttaa actcgtcccc caccgaagac
     1981 aggagatccg catgcttggc cacgaccccg gccacctcag actcagggca ggccgacacc
     2041 tcacgcagcc aattcttccc gaagaaaccc acacccaata ccagcacgcg aagcttgtcc
     2101 atgctaacct cagcgctcag cagtcagcga tcaggtttca gcttgctgaa tgccggccgc
     2161 ttcacaattc acagggcggc caggatacct ccgtcgacat agatgacctg accgcagacg
     2221 aaatccgagg cccgcgaagc cagaaataca gccgccccca ccaactccga ggggtctccc
     2281 catcggccca tgggtgtccg cccgcggatc caggcgtcga acttggggtc ctcgtagagg
     2341 ggccgattca tctccgtggt gaagtagccg gggccgatgc cgttggcctg gatgttgtac
     2401 ttcccccact cgaccgccat ggccttggtg agcatcttca ccccgccctt ggcggccgtg
     2461 taggccccga tcgtggggcg gcccacctcg cacatcagcg agcagatgtt gatgatcttg
     2521 ccagccttcc tggcgatcat tccatgcacc acctgcttcg tgaccaggaa gaggccggtc
     2581 aggttggtgt ccagcacctc ccgccagacc gactcctcga actgctccag gggacttcgt
     2641 cgttggattc cggcgttgtt caccaggatg tggatcgggc cgacttctcg ttccagcgcg
     2701 tcgatcttct gcttcacctg ggcggtattc gagatatcga agcacgaccc gaacaccttg
     2761 aggccctcgc ccgagagcgc ggtgaccgcc ttgttcagct tctcctcgtt cctgccgttc
     2821 aggatgaggg tggcgccgga ctgacccagc ccccgggcga tccccagccc gaggccttga
     2881 taggagccgg tgatcagcgc cacccggccg gtgagatcaa acagttggct ggccatgaga
     2941 tgaatccccc aatgaggtgg aacggatcaa gtggtactca gcctgattcg gacgatccgc
     3001 gctcacttga acttgttcac catgtcctgg atgcctggga ttcccgccat cagatagtgg
     3061 tagtcttcct tgaagttctg caccaggcca cgccgactct tccccacgag gatcgtgaag
     3121 atgtactctt cgtggccggg cgacgtgacg gtcggatgat accctcggtc gagaaggaag
     3181 gtatccccgt tccgggtcat gaaacacttc gcggagccat ctggctgaaa gcaaagctgc
     3241 gccccgaagc cggtttctgg gcggaagcga taatggtacg tctcctcatg ggccgtctcc
     3301 cccgatcccc ggtcctcgtc gtgcttgtgc ggcggatacc cggaccagca cccatgatca
     3361 caatagagtt cgctgatcag gagattgccg gcccgcccgt tcccgttctg gcccaggagg
     3421 tggaagatcc gccggtgcga tcgtgtctcg atggaaccga cgttcaccat ctcgacctct
     3481 tcgggtggaa cgtgaaacgg tggaaacgtc ttgtcgcaga tcccgccggc cacggcgact
     3541 tccgtgccat ccttattcgc ccgaacccgg acgccggcgc cggcgccagc gtacaccgag
     3601 tccgccttgc ccgaccagat gtccttgcgt tgccccacgt cccgaaaggc cgccccgttc
     3661 acgtccacat ccacgttccc cgagagcacc gcgtacagcg tctcgaatcc ttcaaccttg
     3721 gcctggtatt cctccccctt cctgaggcga acaagattga aataggtcaa ggagaggatt
     3781 gtctttcccg acgtgatgat tggctggttc ttgttgtcgt attgccgaat gaggtctggc
     3841 attctgcctt ctccctgtag gattgaagga gacttctccg cctttgcacg ccgccccgct
     3901 acggccccag aaaacggtga gggatatagg tggccgttat gggcacgaag gcgaccaaga
     3961 gcatcacgat gaccagcgtc gctatgaagg gatagcagta ccggatcgtc tcgtcgtact
     4021 tgcacccggc aatgctggtg gcgacgaaga gcaggaccgc caccggcggc gtgacggcgc
     4081 ctatctgggt cgcgatcgtc atcagcaggc cgaagtggaa tgggtcgaag ccgaacgcct
     4141 tcgtcacgta cgtgcagacc ggaatcagga tgatcaggac ggccagggat tcgatgaaca
     4201 ttcccatgat gaagatgacc gcgagcagga ccagcaagat caccattcga tcattggaga
     4261 tgctcttgag gagatccagc acgatgacgt tgaagtcaag ataggacagc agccacccga
     4321 aggccccggc gacggagatg atcccgacca ccgtggttgt cgtgatggcc gcgctcacca
     4381 ggatcttcgg caagtcgcgg agccgcagcg acttgtacac gatgaaggac acgatgaagg
     4441 cgtaccagca ggcaaccacc cctgcctcgg tggcggtgaa gacgccgctc aggatgccgc
     4501 ccaggatgat cacgggcgcc agaagggccg accagacttg acccaaggcc tttagcatcg
     4561 caataaggct gaaccgtccc gtcgtgattc ggagctccgg gaactgggga aggagcgcgt
     4621 gcacgtagat cgttcccatg agccctatgc cgatcaggat ccccgggatg atgcccgaca
     4681 ggaagagtcc cccgatggag gtgccggtca tggacccgta gatgatcatg gtcatgctgg
     4741 gcgggatgat gggcccgatg gtgccggccg cagccaggag ggacgccgcg aagcccggct
     4801 tgtatccccg atccttcatc gaggggatga ggatggagcc gatggccgac gtgtcggcca
     4861 ccgacgaccc agacactccg gcaaagatca tactgcccac caccgacgcg tggccgaggc
     4921 caccgcgcag gtgtcccacg acggtctctg agaaggcaac catcttcttg ctgatgccgc
     4981 cctggcacat cagctccccg gccaggatga agaacggcat ggccaggagg ctgaaggagt
     5041 ccagcatggc gaacatcttc tgggggaaga tcgccaggac gactccgggg ctcgccagca
     5101 tacccaatag accggcgatc ccgatgccga ggagcacggg cgcgcccccg aagagcagga
     5161 ggaggagcgc gacaaagagg ccgatcagca tggcgctcct cctgtcgcct cacccgaccg
     5221 aaggagcagg accgaccccg cgacctttcg caaggccagc agcgccatgt aggaccagcc
     5281 caccgccagg ctgaggtaca cgcgatccat ccgcagaccc atggccgggc tgtcctgatt
     5341 gctcgccacc ctcatgatga ccgtggtgta tgccacgatg gctcccgcca ggacgagcca
     5401 caacaggtct atggccaggg tcatgacgac ctgagctttg gggggcaacc ccttcacgaa
     5461 gagattcatc ccgatgtgag agccatggtt gtaggccacc gggatcgtca ggaagaccat
     5521 ccagatgttg gcatagacca taaactcctc gctccagctc agcgattgat tcaggacgaa
     5581 ccggttgaag acctgaagac cgcccacgat cacgatcgag aagaacagca agaagatagc
     5641 ggcctcgaac gcgcgattca cgcacgaaag gaccttgtcg agcatcgggc tctcctacgg
     5701 gactgatcga aggtatttct cgcgggagac agatagggga gagggaggcc gttttgcctc
     5761 cctccccttg catccgaggc ctcggatcgc tcagtcggtg gcgcggatca tctgcagcag
     5821 atccgtcgcc ccgcgctctt tggcaaaact gtcctggatc gcgatggcgt tcttccggaa
     5881 gctgcttgtg tcaatcttgt gaatgaccat tcccttggac ttgaggaagt caaaggcttc
     5941 ctgctcccgc agggggccca gagtccgctg aacgtctcgg gccatccggg cgccttccag
     6001 gatgactgcc ttctccttgt cggtgagctg agcccaatcc ttcttcgaat aggcgaacac
     6061 gagtggccca aacaggtgct gcgtcagagc gatgtacttt gacacctcgt agaatttctg
     6121 ggccttggtc acggcagagc cgtgctcgta gccatccagc actcccgtct gcatggcggt
     6181 gtagacctcg ccgaaagcca tgggcgtggc attggcgccc atggcgttca acgcagccac
     6241 gtaggtgggc gtctggattg tccggatctt caaccccttg agatccgccg gcgtcttcac
     6301 ctctttcttg gccgtgacga cattccggaa accccagcta tccatccagg ccaagacctg
     6361 gaagcctgtc tgatccagat ccttggccag ggcctcgcca accttgccat ccaggaccct
     6421 gtgggcgtga tcgtagctct tccagaggaa tggaagatca agcactgcaa tcttcttgtt
     6481 gaagttgttc agaatcgccg tcccgctgat caccgcagtc gcgccggagc cgagctgcat
     6541 cccctcgtac accgcgcgct cctctccgag agcgttcgcg gcgtagatct tgacctccag
     6601 cgtgtcggaa ttgtcctcga tccacttctg gaagatccag gccgccgtgt ggatctccga
     6661 ggcaaagtcc gcctgatgac tatgggacag cttgataacc ttcttggcgg cgagcgccgg
     6721 tgaggtggtg gcgaggacca aacccactaa gagtcccatc gtcaggaaaa tgaggctggt
     6781 cggccgtttc atatgatctc ctccttcgcg tcgaacgaat ctgtgccgct gcgcgcacag
     6841 cagctcgtga tactcccggc ggggaacctc accacctcct ttcgcggcgt cccatgcatg
     6901 agtcccttca gcctcaggcg gggccggccg gcgatcgcca tggcaggtac gactccacct
     6961 gtaatcgaac cttccgaatg tgctcttcca tcgccgctgc tgcccggtcc gggtccctgg
     7021 cccggatccc ctcgagcacc gcttcgtgct cctgcagcgc gatcacgaat cgtccgtcct
     7081 gaggcgtttc gtggctcttc gagaggctga cgatgtccgg tgtgagcagg ctgatgaggg
     7141 aacggaggac cgagttacgc gcacactcgg cgatccggag gtgaaacctc aagtccactt
     7201 ccagacccga agctccggac tgcgcctgac tgcaaaaatc ctcgtgaatt cgcccaaggt
     7261 ccgcgatctc cgcatcggtg gcccgctccg cagccagcct ggccgcctgg atctccagga
     7321 gggcgcgggt ctccatgagg gattggaagt cctccttctc caggttgagg acgttcgaga
     7381 tcagcccctc cagtgccttg gcccccaggc tggtcaccat tgtcccgctc tgggggatcg
     7441 tcttgaggat gccgtagaac tcgagctttc taatcgcctc acggatatgt ccccggccga
     7501 cccggaaccg ctcggccagg gcccgctcgg ccggcaggcg gtctcccggt cgcaggacac
     7561 cactggatat cagttccttg atctgctgaa tgatgatttc cgaaggagtc ttgaggtcaa
     7621 ttccccggaa gtgctcgatc acctggcgct tatccgccat tgcacgagtc ctccgtgaaa
     7681 ttggttgact ggcaactggt tgaccagatt gattaagatc attacctgta acgaccatcc
     7741 cgtgtcaaga agatctccga gctggtctgg ggacaagaca agatggaagc ggggcgagtc
     7801 tgatcggcct caaccggatg ccggagacat cttgatagcc agcgtgaatg cacactctac
     7861 ggaggatcac tgagtgtatc tgctgggctt tcgtgcggta tagaacgttg cctgcgtgta
     7921 cgccgaggtg cggtagcccc cggtacccac acaccgtgcg tctattgttg ggccaaccta
     7981 attatgagcg atttcttcaa ggaaaagggg atgccctggg atgacttcct catcacgcct
     8041 ccgtgcggcg gatcccgagc gtctcccaca tccatgcggc gtcgtaccag ggtttcccct
     8101 gttgccgctc gcggtcctga gcattctcga tctctcggag cagttggatg agtcttggac
     8161 gcagaaggac atcgcgggcg gcctgtctcg gggtccggcc gtcgagggcg ggaatctcct
     8221 ggtcgagcca ggccgtgaag tgccggtgga gtgtctgccc cagtagacga gtctggactt
     8281 ccggcgggat ctccgaagaa gcctcccggc cgcgccggcc ctcgggcatc gtgcgcagtt
     8341 tctccatcgg atcttcgatc acgtcggcgc gtaagaggat ggcgtccccg gccagctctg
     8401 ccagtcgcgc ctttgcccag gccaggcgct cggccgacat cgtctccacc gccaggtcgt
     8461 tcccgtcgat ggtaaggctg gccagcccag gtacccccgc cttgccgtcg gcatccagcc
     8521 ggtctgtgcg caacatgagg ccctcggcgg ggagcgccgc ctgtttgacg tatcgctctg
     8581 cgggaccgat cctgatccag gcaaagtgcc gcgcacctgc aggctcgtcc gggccggtcg
     8641 tctcctcgaa gtccggggca gccgccatgg tcgtcaagag ccgatgggga tcgcgcacct
     8701 cgtagcgtag ccggcccagc atgaccggat ggccctcggg cgtatggacc ttgggcggtc
     8761 tggcttcctg cgctaggtgc tgggcatacc ggtggaagat aagcgattcg gcgcggaaga
     8821 aatcccgcca ggcggcgccg gggtggtcgc ggcagtggtc ctgatatcgc ccggtgacgt
     8881 ggctgaccag gccctcccga tccctgggac ggaagagcgt gatcgtcccg gagagaaacg
     8941 gctctccctt gaccaggatc atccgggcgc ccaggaggtc ccaccgggcc acggaaaggg
     9001 agccccggac ctcccgcacg gtgcgggtct ctcccgtgaa gacatcccga agcgtaagac
     9061 tccgcccggg gtccagatcc agcacctcat gcagaccgac cgccgcatcc tgccactcct
     9121 ccaggatggc ccgctcgtct cctggcaggg tgtgaccacg ctccttcaag aaccgagcga
     9181 tgactgagcc ccccgaggac agagggaagt cgtggatcag ccattccagg aaggcgggcc
     9241 agtcggcctc gactccgcga gaatcgaggg caagtctctt ccggtcggca tcgaattgtt
     9301 ccccgaagaa ctctcgcgtg gcgcgatggg catcggcgtg agggatcgag gtctctacat
     9361 aggccatcag ccgctgtcgc agccgcccat ctgggcccag cggcgcctgc gcatccggcc
     9421 cgcgctcgct tttcggcagg cagcacttct tgtacttctt cccgctgccg cacgggcatg
     9481 ggtcgttcct accgaccttg gtgcccccgc gatcctgtgc cgtcagcgct tccagccgcg
     9541 cctgggcgga gaccggctcc ggccgatgcg tggctcggac cccctggact cggaccagtg
     9601 cctcctctat ccaccccagg aactccccac gggccttcac ccggtagaag cgcgccttcc
     9661 gacccagcgc gacgactcgc cggtacatct cctcggcggc ggccaggtcc ccccgctcct
     9721 ctgcgaactg ccccagggtc gcataggcat cggcggcggc agggtccagc tccagcgcgc
     9781 ggcgcagggc tgcttcggat ttctcggcct ctcccagcag ccgcaggacg ttcgcgtagc
     9841 caatgagaag ctcgggatcg tccggccggc ttgccagccg cctttcgtag tccgcccgcg
     9901 cgtcccgagg atgcagccga cggccatccg agaggcccat ccgggcgaaa cgaaacgggc
     9961 cctcctgaag gacctccacc ccctcctgtt cgcttcgctc catgaggagg gccaattcgc
    10021 tcatcagcag aagctgggtc ctctggggga gactgtagtc gtcttcgcgc ccgcagcgct
    10081 tgcagcggat cttgtccagg ataaatggct ggaactcctc ggcctccgtt gcctccggat
    10141 ccaccaggat ctcgcggacg acgtaattgt atgtccgccg gcagccattg catgtgagcg
    10201 gcagttccag gctcgcgtcg ggctcccgat cggagtcctg cgctgcctcc atctcctgct
    10261 ccacccgctc ggcgaggcgc ctgcgaatgt cttcagcccg cggatcggac acctcgttga
    10321 gctgacacag caaggcaaac gcccgctcgg ccgcagcttc cctttcctga agctcccggc
    10381 ccagcggctg gatgaattcc cgggcgccga ggatctccac ggcacgaaga agaggctcgg
    10441 gcgcatggat ccacagctcc tcaaaccgcc ggcagatcgc cgccacgacc cggcgcgacg
    10501 ggagccggat gcacgtgttc aggcaatcct ccaccaagac gggatcctcg gtggacccca
    10561 gccgttccac gacggcatcc accgccgcct ctccgatctc cgccagagca tccacggcgg
    10621 cctccgccag gtccggatgc tctcgatcac ccaggaggcg ggcgagcgcg gccacccccg
    10681 ctggggaatg ccatgctgcc agcaagtcta tggcggttgt cgcctgaggt cctttcagct
    10741 cgatggttcg cagacaggca tcagtccagg catcctgggg tgcctccgcc gccaactgat
    10801 cgaacacgga tcccgccgtc tcgggggccg cgtccgccgc atcgctcaga agccagccaa
    10861 gtcggtcttc gagcgatggc gggagcgtca cgcttcgctc ccgacccgct cgcgagatcc
    10921 gcaccaggtc caggagcatc agtgccgagg cctccctggc cttatccagg gcatctccga
    10981 gtcgcttgga tcgtgcgccg agagtttcca cgagggcaag cgagggtccg gcggcggcgc
    11041 cacgcttcgc ctcgccctcc agccaggccg cgatgggcgc cagcgcctcg atgagacgca
    11101 cccacctgcc cttgcggcct gcccgggatg cctcgctcgt gagccggagc ggcaccagct
    11161 ccgagagcat tcccagcaac gcctcgtgta gaccgggcca ggaagactcc cggtccggat
    11221 tcaaggcctc tcgcagctcc tgaacatccc cgtcgaactc tgctgcgggg agcagcgcgg
    11281 agagcagcgc atccgcctcc tcgccctgcg gagattccag ccaccggtca gctaccaggg
    11341 gaagtgcttc cttgggatcg gcctggacga aggcgccggc aagcagcgac gcggccggtg
    11401 cggctcctcg gagtgggagt tgttcgaatg cttcgcggag cagccgggtg gcctcggccc
    11461 ccttcaaggt agacagggcg agccagaccc ccggatcgca tcggaggggc ccctgcgcgc
    11521 tgcgagcccg gaggtaatcc aggacccggc tgtcacccag ctcggccagc gccacggcac
    11581 acggagcccc gggcatatct ttgccctgac gaaggcggtc caggatggca tcggccagcg
    11641 cagagatccc ccgcttgccg atcaggacgg ccgcagcccc cgacaagata tcgtcggcgt
    11701 cgtccaagca ccttcggagc acctcgaaag gaatctcgag acgcagatcg tcgagacgac
    11761 gcagggccca ataccggact ggaggcgctc ccctccccgc aagtcggagg tagtcggccg
    11821 ccgtccaaca gggatcggcg atggaaggga accctccagg ggaattctca ctcacggatc
    11881 tcatcctctt tgggtgaggg gcagagggcg cggtctcgtg tcgcggaccg ccgatcggcg
    11941 tgggtgccgc ccgaatatgt catccggagg acccgggatg cgcctggcgg cgccagcaga
    12001 aggatcgacg cccttcctcg ccggcgcccg tcaggataac cgggctagat tcccgccggc
    12061 agcttaaccc caaaccgctc cgccaccttg cgaaggttct gggccgtccc ctccggcagc
    12121 gggatgccgt tcttggatcg ctcctcctgc acgcggtcct cgggctctcc cggaatgaga
    12181 acttccttga agccctccgc tggtggcagc gctttcagac gatcgatcag gccatccacg
    12241 tgttccccgt atccctccac atcggtgaac gtgccgatgt tgaccgcggc cacgacgctg
    12301 ttctgccggt ggcgcatggc cccttctttt cccaggagcg ccggctcgag cagcgggtta
    12361 tttacgaaca ggctggagag acactcgaac atcatcgcca ggcccgaccc cttgggtccc
    12421 ccggccggca ggagaatggt ggcaagcttg ggatcggcgc agggcttccc atccttgtcc
    12481 agggcccagc cgagtggaat ggaggccccc ttgtcgatcg ccaggtccag cttgccgcgg
    12541 gccgcaacgc tggtcgccat gtccagcatc agatgactgt gccgcctccc cggaacgcag
    12601 acggcaatcg ggctgttgtg gacgccgatt gcccgcgccc cgtgcggcgc catgttggga
    12661 ggattgcaca cgatggcaat gcccgccatg ccctgcttgg ccgccatcag ggggtagtat
    12721 cccatggcac cctgatgcaa ggtgttgcgg atcacgaccc agccgatgcc gacctctcgg
    12781 gccttctgga ttgcccggtt catcgccggc accgtgacca ccggtcccgg agcataatcg
    12841 gcgtccatga gcagagtggc cgccgtctcc ttctccaccc ggatgttcgg ccggggtttc
    12901 atgagcccct tgtccaccag ctcgacgtac cagggtattc gcagcacgcc atgcgagtcc
    12961 accccgcgga gattcgccca caccagcaca tccgcctgga gctccgcccc ctcgggtgtg
    13021 aacccggcac acgcgaacac ttctcggctg aagtcct
//
LOCUS       DBNGZC010000142         7116 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1087456, whole genome shotgun sequence.
ACCESSION   DBNGZC010000142 DBNGZC010000000
VERSION     DBNGZC010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7116)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7116)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7116
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1087456"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="AC16G0_02770"
     CDS             complement(<1..1283)
                     /locus_tag="AC16G0_02770"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5098574.1"
                     /translation="MSADRGKSTKQGWGVVPRRTFLKGAMAVAGAAGLGGIPGILAAG
                     QAPAYPKGTKLHLLTRINFIPAGDTLFIAQAEEFGKQMGVEVQVERIGENDVTTRSTA
                     AIVAKSGPDIIMLQNNFPHLLANGLADVSDLAEALGKGQGGFWDLSKANAYVGKQWLA
                     VPYGVLSNTMNYREDWLKEAGFSKFPDTYEELREVGKKLKAMGHPVGQCFGHSVNDPN
                     SWCYPFVWAYGGYEVEKDGKKVGLDKKGTLEAIKLNTAMWKDCFDEGGLSWDDASNNR
                     AFLAGSISITNNGASIYFVGKDKFPDVAKVTNHAPNPRGPAGRFYHMATHSSAVMKYS
                     KNQKLAKEFIRWYMDKKQYEPWFIAMSSYMIPPTKVWFEHAAWTKDPKLTPFRDSIKD
                     ARHLGYAGPPSSKASEALAKFIIVDMFAKAIQGTSP"
     gene            complement(1368..2711)
                     /locus_tag="AC16G0_02775"
     CDS             complement(1368..2711)
                     /locus_tag="AC16G0_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013653798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HIR5098575.1"
                     /translation="MNLYRLLQERAATKAPIRIGLIGAGKFGAMFLAQARLTPGMHIL
                     GIADLNVERARAACRSTGWPAEQFAAPSFAAALQTGGTHLTDDAMALIAADGLDVMIE
                     ATGVPAAGTKHALAAIAHQRHVIMVCVEADVLTGPLLAKRAQEAGVVYSLAYGDQPAI
                     ICELVDWARACGFPVVCAGKGTRYHPSFHASTPDTVWDNFGWSKETVAKSGANPKMFN
                     SLIDGTKSGIEMTAVCNATGLVPPPDGLGFPPVPAAEIAKFLKPKSDGGTLAYKGTVE
                     VISSLNRDKTPVPHHLQQGVYVTYEAPSEYTQRSFNEYWLLPDETGKYAGLYRPLHLI
                     GLELGVSVASIMCRGEATGSATCWNADVVATAKRDLKAGEVLDGEGGYMVWGKIMPAA
                     TSIAKNGLPLGLAHMKLIRPVAKGQLVSWADVEVDPRDPTVAFRREMEKTFAPKK"
     gene            complement(2996..4330)
                     /locus_tag="AC16G0_02780"
     CDS             complement(2996..4330)
                     /locus_tag="AC16G0_02780"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098576.1"
                     /translation="MNDRRRWSCAIPSVSIPRRAFLKGAAAAGAATLVGIPGILQAGQ
                     APAYPKGTKLHLLTRLNFFPAADKLFIAQADEFGKQMGVEVQIERIGQNDIITRTTAA
                     VVAKSGADIIILHNNSPHLFADALIDVSDVAEAIGKDGGGYYDLFRANAHVGNRWLGV
                     PHVALSTAMNYREDWLKEAGYTKFPDTWDEFREMGKKLKAMGRPMGQAFGHSENDPNT
                     FSYSLLWSYGGYEVEKDGKTVVLDKKGTLEAIKFNTVLWKDAMDEGGISWDDSSNNRA
                     FLAGSISVTTNGPSIYFVAKEKMPDMYKAMNFAPHPRGPAGRFYHLPVHSSTVMKYSK
                     NQKLAMEFIKYYMAKEQYDKWIDIMEGYGIGPTKVWYNHRAWFRDPKLAAFRDPIKDA
                     RHPGFAGPPSRKASEALAKFIIIDMFAKAIQGAPPEDALKWATDELKKIYGA"
     gene            complement(4465..5802)
                     /locus_tag="AC16G0_02785"
     CDS             complement(4465..5802)
                     /locus_tag="AC16G0_02785"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098577.1"
                     /translation="MGAERRKGGRGQWPSIPRRTFLKGSAAVAGAAALTGIPGILRAG
                     QAPAYPKGTKLHMLQQINFVPAGDKVFIEQAAEFSKQMGVNVQVETIAQNDVPTRAMS
                     AVEMKSGADIILIQNNFPHLIADGVVDVSDVAEPIGKAQGGYYEVSRANAFAGGRWIA
                     VPHDVYSWGWNYRESWLREVGYNKFPETWDDFRVMGKKLKANGHPVGQAFGHSTNDPN
                     NYCYALVWCLGGYEVEKDGKTVVLDKKGTLEALKLNTAMWKECMDEGGLSWDDASNNR
                     AFLAGTLSVTGNSPSIYFVARDKFPDVYKDMNHAPMPKGPAGRFYQLPTYSSVVMKYS
                     KNQKLAKEFIRWYMAKEQYEKWFEVMDTFSIPPTKMWYDHPVWVKDRKNVIFRDVIKD
                     SRWAGFAGPPGRKASEAMAKYILVDMFAKAIQGTTPEEALKWGTVELKKIYGA"
     gene            complement(6315..>7116)
                     /locus_tag="AC16G0_02790"
     CDS             complement(6315..>7116)
                     /locus_tag="AC16G0_02790"
                     /inference="COORDINATES: protein motif:HMM:NF028352.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide-binding domain containing protein"
                     /protein_id="HIR5098578.1"
                     /translation="LRGHATQAAKTLKAWREGAPGVVLADAVTPRDLERLASLLVREG
                     LIQVVAGSAGLAMALSAVLAWPRRKRGNPPAAQGPVLLVLGSPNPVSLEQAAWVERQG
                     HATAIRATIREVVAGGDRFRRELDRVVGKVQAEILAGRDTLLTLAQRQSSSSKTPRPL
                     RPSASGALSEFLGRATRLASRGSSLGALVLCGGDIAIAVCRALRAGGIELQGEVEAGV
                     PWGRMVGGELPDLPTITKAGGFGEPATLSRAIRFLRHGCRSRTTRRAN"
BASE COUNT         1184 a         2333 c         2184 g         1415 t
ORIGIN      
        1 ggcgaggtgc cctggatcgc cttggcgaac atgtccacga tgatgaactt cgccagcgcc
       61 tcggaagcct tcgaactcgg cggtcctgcg tacccgaggt gccgcgcgtc cttgatggaa
      121 tcccggaagg gggtcagctt ggggtccttc gtccatgctg cgtgctcgaa ccacaccttg
      181 gtcggcggga tcatgtagct tgacatggcg atgaaccagg gctcgtactg tttcttgtcc
      241 atgtaccagc ggatgaactc cttggccagc ttctggttct tggagtattt catcacggcg
      301 ctgctgtggg tcgccatgtg atagaagcgg ccggcgggtc cccggggatt gggggcgtga
      361 tttgtgacct tggcaacatc cgggaacttg tctttgccca cgaagtagat gctggcaccg
      421 ttgttggtga tggagatgct tccggccaga aacgcccggt tgttgctggc gtcgtcccag
      481 gagaggccgc cctcgtcgaa gcagtccttc cacatggcgg tgttgagctt gatcgcctcc
      541 agggtcccct tcttgtcgag gcccactttc ttgccgtcct tctccacctc gtagccgccg
      601 taggcccaca cgaagggata gcaccaggag ttgggatcgt tcacgctgtg cccgaagcac
      661 tgcccgaccg ggtggcccat ggccttgagc ttcttcccga cttcccgcag ctcctcgtag
      721 gtgtccggga acttgctgaa ccccgcctcc ttcagccagt cttcgcgata gttcatggta
      781 ttggagagga cgccgtaggg cacggcaagc cattgcttgc ccacataggc gttggccttg
      841 gagaggtccc agaacccgcc ctgccccttg cccagggcct ccgccaggtc gctcacatcc
      901 gccaggccat tggccagaag gtgcggaaaa ttgttctgga gcatgatgat gtccgggccc
      961 gacttggcca caatggcggc tgtggatcga gtcgtgacgt cattctcgcc gatccgctcc
     1021 acctggacct cgacgcccat ctgcttgccg aactcctcgg cctgggcaat gaagagcgta
     1081 tcgcccgcag gaatgaagtt gatccgggta aggaggtgca gcttcgttcc cttggggtag
     1141 gccggggcct ggcccgcagc caggatgccc gggatgcccc ccaaccctgc cgcccccgcg
     1201 accgccatcg cccccttcaa gaacgtccgc cggggaacca caccccagcc ttgcttggtg
     1261 ctttttcctc gatccgcgct catgatgttc tcctcctggt cgtcggtgtg aagagacctc
     1321 gtatgatcgc acgacctcgc tacacccccc ttgcattccc catggcgcta tttcttcggc
     1381 gcgaacgtct tctccatctc tcgccggaag gccacggtgg gatccctcgg gtcgacctcg
     1441 acatccgccc aggagacgag ctgccctttc gccaccggcc ggatgagctt catatgggcc
     1501 aggcccaagg ggaggccgtt cttggcgatg gaggtggctg ccggcatgat cttcccccac
     1561 accatgtaac caccctctcc gtccaggacc tcgcccgctt tgagatcacg cttggcggtg
     1621 gcgaccacgt ccgcattcca gcaggttgcg ctccccgtcg cctcgccccg gcacataatg
     1681 gaggccacgc tcacgcccag ttccaggccg atcaggtgaa gcgggcggta cagcccggca
     1741 tactttccgg tctcatcggg gagcagccag tactcgttga aggagcgttg cgtgtactcg
     1801 ctcggggcct cgtaggtgac gtacaccccc tgctggagat ggtgaggaac gggggtcttg
     1861 tcccggttca ggctggagat gacctccacc gtccccttgt acgccagggt tccgccgtcg
     1921 gacttgggct tcaggaactt ggcgatctcg gccgccggga ccggtgggaa cccgaggccg
     1981 tcgggcggtg ggacaaggcc ggtggcgttg cagacggccg tcatctcgat ccccgacttg
     2041 gtcccgtcga ttaaggagtt gaacatcttc gggttcgccc ccgacttggc cactgtctcc
     2101 ttgctccagc cgaagttgtc ccagacggtg tcgggcgtgg acgcatggaa gctcggatgg
     2161 taccgggtcc ccttgccggc gcagacgaca gggaagccgc aggcccgcgc ccagtccacc
     2221 agctcacaga tgatggccgg ctggtcgcca taggccaggg agtagaccac gcccgcctcc
     2281 tgggccctct tggccaggag cggccccgtg agaacgtcgg cctccacgca gaccatgatg
     2341 acgtgcctct ggtgcgcgat ggcggccagg gcgtgcttcg tcccggctgc gggaaccccc
     2401 gtcgcctcga tcatcacgtc cagcccgtcc gccgcgatga gggccatggc gtcgtcggtg
     2461 aggtgggtcc cgccggtctg cagggcggcg gcaaaggacg gggcggcaaa ctgctcggcc
     2521 ggccagccgg tgctgcgaca ggccgcccgc gcccgctcca cgttcaggtc ggcgatcccc
     2581 aggatgtgca tgcccggtgt gagccgtgcc tgagccagga acatggcgcc gaacttcccc
     2641 gccccgatga ggccgatccg gatgggagcc ttcgtggcgg cgcgttcttg cagcaagcgg
     2701 tacaggttca tgcgtgccct ccgtggttga gccggtccgg gtattcacat cgtcggggtg
     2761 cgggacctca gcgcggagcc gacgattctt ctgcagacat gattcgtgcc gacgcacgtt
     2821 ccatccgggt gggcggatct ctcctgccgg caggcgtaga ccttcttgta ccagagatgt
     2881 gcgagcagtg tcaatcggcg tgaggggcag gtagacctgc ctagacgaac gggccggcct
     2941 ggaagcaccc cggacccccc tcgggcggac ttccaggccg ggagagaagc tgggctcagg
     3001 cgccgtagat cttcttcagt tcgtccgtgg cccacttgag ggcgtcctcc gggggagcac
     3061 cctggatcgc cttggcgaac atgtcgatga tgatgaactt ggccagggcc tcggaagcct
     3121 tccggcttgg tggcccggcg aatcctgggt gtcgggcatc tttgataggg tcgcggaacg
     3181 cggccagctt gggatctcga aaccaggcgc ggtggttgta ccataccttg gtcggcccga
     3241 tcccgtagcc ctccatgatg tcgatccact tgtcgtattg ctccttggcc atgtagtact
     3301 tgatgaattc catggccagc ttctgattct tggagtattt catcaccgtg ctgctgtgga
     3361 cgggcagatg atagaagcgg ccggccggtc ctcgggggtg gggtgcgaag ttcatcgcct
     3421 tgtacatgtc cggcatcttc tctttcgcca caaaatatat gctggggccg ttggtcgtga
     3481 cggagatgct ccctgccaaa aacgcacggt tgttgctgga gtcgtcccag gaaatgccgc
     3541 cctcgtccat ggcatccttc cataggacgg tgttgaactt gatggcctcc agggtgccct
     3601 tcttgtccag gaccacggtc ttgccgtcct tctccacctc gtatccgcca taactccaga
     3661 gcagcgaata agaaaacgtg ttcggatcat tttcgctatg gccaaaggcc tggcccatcg
     3721 gacgccccat ggccttgagt ttcttcccca tttcccgaaa ctcgtcccag gtatccggaa
     3781 acttggtgta ccccgcttcc ttcaaccaat cctcccggta attcatggcc gtggagagcg
     3841 cgacgtgggg cacgccgagc catcggttgc ccacatgggc attggcgcgg aagagatcgt
     3901 aataaccccc accatccttc ccgatggctt ccgcgacgtc actgacatct atgagggcgt
     3961 cggcgaacag gtgcggcgag ttgttgtgga ggatgatgat atccgctccg gattttgcca
     4021 cgaccgctgc tgttgtccgg gtgatgatat cgttctggcc gatgcgctcg atctgaacct
     4081 ccacccccat ctgcttgccg aactcgtcgg cttgagcaat gaaaagctta tctgcggcgg
     4141 gaaagaaatt cagtcgcgtc aaaagatgca gcttcgttcc cttgggatac gccggggcct
     4201 gccccgcctg caggatcccc ggaatgccca ccagcgtggc cgcgccagcg gccgctgccc
     4261 ctttgaggaa tgcgcgtcta ggaatagaga ccgaggggat tgcacagctc cagcgtcgcc
     4321 gatcattcat ttcttctgcc tccctatcca accttcgagt gccatcatgc ggccgggaaa
     4381 acattgcctt tcaggcggag gcggtgtccc ccggcccctg ccgttcggag atccggcagg
     4441 ggccaaggcg atggatctcc ggtatcaggc gccgtagatc ttcttcaatt ccacggtgcc
     4501 ccacttcagg gcctcctccg gcgtggtccc ctggatggct ttggcgaaca tatccacgag
     4561 gatgtacttc gccatggcct cggaggcctt tcggcccgga ggaccagcga atccggccca
     4621 gcgggaatcc ttgatcacgt cgcggaagat gacgttcttc cggtctttga cccaaacagg
     4681 atggtcatac cacatcttgg ttggtgggat cgaaaacgtg tccatcacct cgaaccactt
     4741 ctcgtactgc tcctttgcca tgtaccagcg gatgaactcc ttggccagct tctggttctt
     4801 ggagtacttc atcaccacgc tgctgtaggt cggcaactga tagaagcggc ctgccggtcc
     4861 cttgggcatg ggggcgtggt tcatatcttt gtagacatcg gggaacttgt cccgagccac
     4921 gaagtagatg ctgggggagt ttccggtgac cgacagggtc ccggccagga aggcccggtt
     4981 gttgctcgca tcgtcccagg acaggccgcc ctcgtccatg cactctttcc acatggcggt
     5041 gttgagcttg agcgcctcaa gggtcccctt cttgtccagg acgacggtct tgccatcctt
     5101 ctccacctcg tagccgccca gacaccagac cagggcatag cagtagttgt tcggatcgtt
     5161 cgtgctgtgg ccgaaggcct ggccgaccgg atgcccgttg gccttcagct tctttcccat
     5221 gacccggaaa tcatcccagg tctccgggaa cttgttatag ccgacctcgc gcagccagct
     5281 ctcccgataa ttccagcccc aggaatagac gtcgtgcgga accgcgatcc atcgaccgcc
     5341 cgcaaaggcg ttggcccggg acacctcgta gtacccgccc tgggccttgc cgatcggctc
     5401 ggcaacgtcg ctcacatcca ccaccccatc ggcaatgaga tggggaaaat tgttctggat
     5461 caggatgatg tcggcgcccg acttcatttc gacggccgac atcgcgcggg tagggacatc
     5521 gttctgcgcg atcgtctcca cctggacgtt gacccccatc tgcttgctga actccgctgc
     5581 ctgctcgatg aagaccttgt ccccagccgg aacgaagttg atctgctgaa gcatgtgaag
     5641 cttggttccc ttggggtacg ccggggcctg gcccgcgcgc aggatcccgg ggatcccggt
     5701 gagcgccgct gcacccgcga ccgcagctga ccccttcagg aaggtccttc gaggaatcga
     5761 tggccactgc cctctaccgc ccttgcgtcg ctccgcaccc atgatggcat cctccctttc
     5821 cagatgacct gactccctct gcttctccga tggtgcgccg accgccttct cgccttcccc
     5881 ttcacacctc ccttcggctc gttccgagcg gcaccgatcc gtccgggtct cgacagtctc
     5941 ctcgtccgtt ccgaacccgg catctcccgt cagatgcagc catcccacct gtcatctgct
     6001 ggtttgcgtc agataactgg ctcgacgaat ggatcccgcg gccgtgggca agaccgggat
     6061 cacagttcac cactcagacg gagcgcatgt caaggacaaa ccggcgtcac ccgcttggaa
     6121 gccagacggg agccaagact ccatcgggtc ggctcattgc cttgggggca ggacctggag
     6181 gggagaaaag gaatccagcg ccaggcagga tcgcagccgg agagacgacg tgcctagaag
     6241 acacagggcc tggcagggag agaaggaaaa gaacagtgca gggatgaccg attctgcgga
     6301 ggtcctgccg cccctcagtt cgcccttctg gttgtgcggg atcggcagcc gtgacgcagg
     6361 aagcggatgg cgcggctgag ggtggccggc tcgccgaagc cgccggcctt ggtgatggta
     6421 ggaaggtccg gaagctcgcc gccgaccatt cgcccccagg gtaccccagc ttccacctcg
     6481 ccctggagtt cgatgccgcc ggcgcggagg gcccgacaca cggcgatagc gatatcgccc
     6541 ccgcatagca cgagggcccc gaggctgctg ccgcgggacg ccaggcgggt tgctcgcccc
     6601 aagaactcgc tgagcgcccc gctggccgac gggcgtaggg gacgtggcgt cttcgaggac
     6661 gaggactggc gctgcgccaa cgtcaggagt gtgtcgcggc ccgccaggat ctccgcctgc
     6721 accttcccca caacccgatc caattcccgg cggaagcgat ccccgccggc cacgacctcg
     6781 cggatggtgg cccgaattgc ggttgcatgg ccttgccgct ccacccaggc cgcttgttcc
     6841 aggctcaccg gatttggcga tcccaacacc aggagtaccg gcccctgtgc cgccggagga
     6901 ttcccccgct tccggcgcgg ccaggcgagc accgcgctca aggccatggc caggcctgcg
     6961 gagcctgcca caacctggat caggccttcg cggacaagca ggctggcgag gcgctccaga
     7021 tcgcgcggcg tcaccgcatc cgccaggacc acaccgggcg ccccctcacg ccaggccttc
     7081 agggtcttgg cggcctgcgt tgcgtgaccg cggagg
//
LOCUS       DBNGZC010000143         1667 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2065978, whole genome shotgun sequence.
ACCESSION   DBNGZC010000143 DBNGZC010000000
VERSION     DBNGZC010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2065978"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="AC16G0_02795"
     CDS             <1..982
                     /locus_tag="AC16G0_02795"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR5098579.1"
                     /translation="DRVPRILWLFLGVGLIILSLLGSFPRVSDAAGAYPTKPVTVFNS
                     SAAGSPTDVMARQVAQYARKYLGQPMVVVNKPGGGGGVMFAALFADPADGYTIASLTG
                     AQVTALHAELKKDFSFENLEFIANVQREPNCLAVKADGPFKTLKDMIEYAKKNPSLKV
                     GGQGTGSALHLMVMTLAEQSGTKLTWVPFSGGSESVTNLLGNTVSVISTSPATVNQYV
                     EANQMRILAITGDKRMEHIKDVPTFKELGYDLVMYQYRGFAARKGLPAEAKAKLAEAI
                     RKAVAEPGFKEFMAKNYQPEGYLGPEEFTALARSDFELMGRLLQKLGTKQ"
     gene            1034..1477
                     /locus_tag="AC16G0_02800"
     CDS             1034..1477
                     /locus_tag="AC16G0_02800"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR5098580.1"
                     /translation="MRRGELTISAGMGLLAIAFFGLATFTQEINPIDPGPAFYPRLVS
                     LLLLAFSITQIVISWRGGKGAGGDTGAGLPAAAYVYSLGTLVLSILYISVFDKASYLV
                     TAPGLLVALMLLGGVRKWTILAGVALGYTLFTYYLFGQLLMVPIP"
     gene            1507..>1667
                     /locus_tag="AC16G0_02805"
     CDS             1507..>1667
                     /locus_tag="AC16G0_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR5098581.1"
                     /translation="MKHRFLGKSGLEVSSIGLGCMGMSPIYGEPDEAESIATIQRAIE
                     LGVNFLDTS"
BASE COUNT          308 a          487 c          564 g          308 t
ORIGIN      
        1 agacagagtg ccgcggatcc tgtggctgtt cctgggagtt ggtctcatca tcctgagcct
       61 actcggatcc ttccccaggg tctccgacgc cgcgggagcc tatccaacca agccggtcac
      121 cgtcttcaat tcctcggccg cgggctcgcc gacggatgtg atggcccggc aggtggccca
      181 gtacgcgcgc aagtatctgg gccagccgat ggtggtcgtc aacaagccag gtggaggcgg
      241 cggcgtcatg tttgccgctc tgttcgccga tccggcggat ggctacacca tcgcctcgct
      301 gaccggagcc caggtcacgg ccctgcacgc cgagctgaag aaagacttct cattcgagaa
      361 cctggagttc atcgccaacg tgcagcgaga gccgaattgc ctggccgtga aggctgacgg
      421 gcccttcaag acgctcaagg atatgatcga gtacgccaag aagaacccga gcctgaaagt
      481 gggcggccag ggaacaggat cggcgctgca cctgatggtg atgaccctcg cagagcagtc
      541 cgggacgaag ctgacctggg tcccgttcag cggcggcagc gaatccgtca ccaatctcct
      601 ggggaacacc gtctccgtca tcagcacctc tcccgccacg gtgaatcagt acgtggaagc
      661 gaatcagatg cggatcctgg ccatcaccgg ggacaagcgg atggagcaca tcaaggacgt
      721 gcccacgttc aaggagctgg gctacgacct ggtgatgtac cagtaccggg gattcgccgc
      781 gaggaagggg ctgcccgcgg aggcgaaggc caagctggcg gaggccatcc ggaaggccgt
      841 cgcagagccg ggcttcaagg agttcatggc caagaactat cagcccgagg gatatctggg
      901 gccggaagag ttcacggcgc tggcccgatc tgatttcgag ctgatgggcc gcctgctgca
      961 aaaactgggg acgaagcagt agccggaaag aatggcggcg gcttcaggct tgcggcgctg
     1021 gatggaggcg gacgtgcgca ggggcgagct gacgatcagt gcgggcatgg gtctcttggc
     1081 gattgccttc ttcggcctgg cgacgttcac gcaggagatc aatccgatcg atcccggccc
     1141 ggccttctac ccgcggctgg tgagtctcct gctgctggcg ttctcgatca cgcagatcgt
     1201 catttcctgg cgagggggga agggcgcggg aggggatacg ggggcgggac tgccggctgc
     1261 cgcctacgtg tacagtctgg gcaccctggt cctgagcatc ctgtatatca gtgtgttcga
     1321 caaggccagc tatcttgtga cggcgccggg tctgctcgtc gccctgatgc tcttgggcgg
     1381 cgtgcgcaag tggacgatcc tggccggggt ggccctgggg tatacgctct tcacctatta
     1441 tctgttcggc cagttgctga tggttccgat tccctgagtg gacaggcgga ccttgggggg
     1501 gtatccgtga aacatcgctt cctgggaaag agcggcctgg aggtctcctc catcggcctg
     1561 ggatgcatgg gaatgagccc gatctacggc gaaccggatg aagctgagtc cattgccacg
     1621 atccagcggg ccatcgagct tggcgtgaat ttcctggaca cctccga
//
LOCUS       DBNGZC010000144         1890 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1185745, whole genome shotgun sequence.
ACCESSION   DBNGZC010000144 DBNGZC010000000
VERSION     DBNGZC010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1185745"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="AC16G0_02810"
     CDS             <1..356
                     /locus_tag="AC16G0_02810"
                     /inference="COORDINATES: protein motif:HMM:NF022750.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HIR5098582.1"
                     /translation="LSLLYYVRSLPLAVGATVRAKVFASRKNWDLEVRVLDRERLDTI
                     LGQRETLVVEPLLKFEGVFQQKGRVIVWLTDDPERVPVRMQSEIKVGSFVSTLTRREV
                     GGGRASLDDPGRVAR"
     gene            353..1315
                     /locus_tag="AC16G0_02815"
     CDS             353..1315
                     /locus_tag="AC16G0_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015170347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5098583.1"
                     /translation="MSDRPISLVTGAAGFIGSHLCERLLREGHGVVGLDAFFDYYPRR
                     LKEKNLAASLAHPQFRFLELDLNRADLAPIVREADQIFHQAGQPGVRPSWGKDFDVYV
                     DCNIRATQRLLEAAKESSRLRRFVFASSSSVYGDTRDLPVRETSLPQPFSPYGVTKLA
                     AEHLCGLYHANHGVPTVALRYFTVYGPRQRPDMGFNKFIQAALQDLPIVVYGDGEQTR
                     DFTYVTDAVEANWLALEGAAAGKIFNIGGGSSCSLNQVLRTLEEILGRPIRREHRPAQ
                     PGDVRHTWADTSRARDHLGFAPRVSLREGLASEVEWLRTERETP"
     gene            1415..>1890
                     /locus_tag="AC16G0_02820"
     CDS             1415..>1890
                     /locus_tag="AC16G0_02820"
                     /inference="COORDINATES: protein motif:HMM:NF015655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HIR5098584.1"
                     /translation="MDSLVPQLPERLAAVGCQHPEQAAKNVESLALDAETRSQLQAVL
                     PALFTGLRRVPDPDLALNNLERFCQAVLDRRFLLGLFRDNSRVLHLALTIFGSSQFLS
                     DILVRHPLFFEWLLEPGVIRQPKRKEDLAAEARQLVAAAPILDRKWNALRGFKARE"
BASE COUNT          301 a          602 c          658 g          329 t
ORIGIN      
        1 agctgtccct gctgtactat gtgcgcagcc tgccgctggc agtcggtgcc accgtcaggg
       61 ccaaggtatt tgcgagcagg aagaattggg acctggaggt gcgggtgttg gaccgcgaga
      121 ggctggacac gatcctgggc cagcgcgaga cgctggtcgt cgagcccctg ctgaagttcg
      181 agggggtctt ccagcagaag ggacgggtga tcgtgtggct cactgacgat ccggagcggg
      241 ttccggtgcg gatgcagagc gagatcaagg tcgggagctt cgtctcgacg ctcacccggc
      301 gggaggttgg gggaggccgg gcgagcctcg acgaccccgg gagggtagca cgatgagcga
      361 ccgtccgatt tccctggtca ccggcgccgc gggattcatc ggctcccatc tgtgcgaacg
      421 actcctccgc gaggggcacg gggtcgtcgg tctggacgcg tttttcgact actatccccg
      481 gaggctcaaa gagaagaacc tggccgcatc cctggcgcat ccgcagtttc gcttcctcga
      541 gctggacctg aaccgggccg atctggcgcc gatcgtgagg gaggccgacc agatcttcca
      601 ccaggctggg cagcccgggg tgcgtccaag ctgggggaag gatttcgacg tgtatgtgga
      661 ctgcaacata cgggcgaccc agcgcctgct ggaggccgcc aaggagtcct cacggctgcg
      721 gcgattcgtc ttcgcctcct cctcctcggt gtacggtgac actcgcgatc tgcccgtgcg
      781 ggagacgtcg ctgccgcagc ctttttcccc ctacggtgtg accaagctgg cggccgagca
      841 tctgtgcggc ctgtaccacg cgaatcatgg cgtgcccacg gtggcgctgc gctactttac
      901 cgtgtacggt ccgcggcaga ggccggatat gggcttcaac aaattcatcc aggcggcgct
      961 ccaggacctc cccatcgtcg tgtatgggga cggcgagcag acacgggatt tcacctacgt
     1021 gacggacgcc gtggaggcga actggttggc cctggaaggg gccgccgcgg ggaagatctt
     1081 caacatcggc gggggctcga gctgcagcct gaatcaggtg ttgcggacgc tggaggaaat
     1141 cctgggacgc cccatccggc gcgagcaccg acccgcccag ccgggcgatg tccggcatac
     1201 ctgggcggac acgagtcggg cgcgcgatca cctgggcttc gcgccacggg tgtcgctgcg
     1261 cgagggtctg gcgagcgagg tcgagtggct ccggacggag agggagaccc cctgatctct
     1321 tgccggacat ccgaggacgc tcgacggtat cggcgcagac tgcgtcgacg ggaacgccgc
     1381 cgggttcatg cagtgagcca tgcggggtgt caccatggat agcctcgtac cgcagctccc
     1441 cgagcgtctc gccgcggtcg gctgccagca tccggagcag gccgccaaga acgtcgaatc
     1501 cctggccctg gatgcagaga ctcgctcgca gctccaggcc gtgctcccgg cgctcttcac
     1561 gggtctccgc cgcgtcccgg acccggacct ggccctgaat aacttggagc ggttctgcca
     1621 ggcggtgctg gatcgtcgat tcctcttggg ccttttccga gacaactcgc gcgtcctgca
     1681 cctggccctc accatcttcg gcagctccca gttcctgagc gacatcctgg tgcgccatcc
     1741 gctgttcttc gagtggctgc tggagccggg ggtcatccgc cagccgaagc gcaaggaaga
     1801 cctcgccgcg gaggcgcgcc agttggtggc agccgcgccc atcttggatc ggaagtggaa
     1861 cgccctgcgc ggcttcaagg cccgggagat
//
LOCUS       DBNGZC010000145         3096 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2066218, whole genome shotgun sequence.
ACCESSION   DBNGZC010000145 DBNGZC010000000
VERSION     DBNGZC010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2066218"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="AC16G0_02825"
     CDS             <1..354
                     /locus_tag="AC16G0_02825"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5098585.1"
                     /translation="NISSGAGRSYSLTGIQAYASAKAGLIGFTRQTARELGAFGVRVN
                     CVAPGFVRSNPASERQWQAMGAEGQRRLLDSIALKRLGKPEDIAKAVAFFASDDAQYV
                     TGQTISVDGGMWMLG"
     gene            498..1676
                     /locus_tag="AC16G0_02830"
     CDS             498..1676
                     /locus_tag="AC16G0_02830"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HIR5098586.1"
                     /translation="MSHPFDTILERRNTACLKWDGIEGRYGVKGDDLLPMWVADMEFR
                     PPEAVVEALVSRAEHGICGYPADLEAYHESIRGWMRRRHGWEVRGEWIASAPGVVCAV
                     NLLIRTLTNRGDGVVLQPPVYYPFFRGVEENGCRVLSNPLRYDGTRYVMDFEDLERKL
                     DQAKLLVLCSPHNPVGRVWTPEELLRLGQLCLARGVVIISDEIHCDLTFPGHPHTAFP
                     TLSDALAQHSIVCTSPSKTFNLPGLQPGIIVIPNPRLRRDFLRTLRACGIPDTNVFCL
                     EAVKAAYNHGEPWLKDLLAYLEENHNLLRRFVAKEIPRLRVVPSEGTYLAWLDCRGLG
                     LEPKTLEQLLLIKARLVLNQGYTFGVGGEGFVRMNLACPRAMLEDGLGRLASAIAGVP
                     "
     gene            1800..2759
                     /locus_tag="AC16G0_02835"
     CDS             1800..2759
                     /locus_tag="AC16G0_02835"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR5098587.1"
                     /translation="MKPVVLVTRKLPDAVEDRLRRDYQPRLNPDDRLYSSEELIAKAG
                     GAAAIVPCHTERFSAEVIQRLPDTVRIIANFSVGYDHVDLEAAKARGIIVTNTPEVLS
                     DATAELTILLMLGAARRAGEGERLVRTREWRDWSPSFMVGTQMTGKRLGIVGMGRVGR
                     VTARRARGFDMVIHYHDIRRLPPEQEEGAIFHPTPEDLLPYCDFLAFHCVATPQTRKL
                     LNAERIALLPQGAIVVNASRGVVVDDEALIAALKSGRVAAAGLDVYNNEPNIHPGYRE
                     LPNTFLMPHIGSATKETRDGMGFRALDNLDAFFAGRGPRDRVA"
     gene            2936..>3096
                     /locus_tag="AC16G0_02840"
     CDS             2936..>3096
                     /locus_tag="AC16G0_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR5098588.1"
                     /translation="MDFRPMLTLAHEAEGWGYESLWVTHGGGRDAFLVLSAYAHVTRT
                     VGLGTGVVP"
BASE COUNT          527 a          988 c         1038 g          543 t
ORIGIN      
        1 aacatctcct cgggggcagg cagatcttac agcctgacgg gcatccaggc ctacgccagc
       61 gccaaggcgg ggctgatcgg cttcacccgg cagacggcgc gtgagctggg tgcgttcggc
      121 gtccgggtga actgcgtcgc gccgggcttc gtgcgctcca atcccgcttc ggagcggcaa
      181 tggcaggcca tgggggcgga agggcagcgg cgccttctcg actccatcgc actcaagcgg
      241 ctgggcaagc cggaagacat tgccaaggcc gtggcgttct ttgcctcgga cgatgcccag
      301 tacgtgaccg ggcagaccat cagcgtggac ggcggcatgt ggatgctcgg ctgacacagg
      361 ggaaaccgcg cgtgcctgtg agcgttcgac cctgaaggga ccgtaggcgg gggcggctcg
      421 cgccagtcgc gcgagcgacc tcttttccgg gtcggatact cgtcgagcaa gcgatgcatt
      481 tatcagcagg agatgccatg agccatcctt tcgacacgat cctcgagcgc cggaacaccg
      541 cctgtctcaa gtgggatggg atcgaaggcc ggtacggcgt gaaaggcgat gaccttttgc
      601 ccatgtgggt ggcggacatg gagttccgac cacccgaggc ggtggtggag gccctggtaa
      661 gccgggcgga gcacggcatc tgcgggtatc ccgcggacct ggaagcgtat cacgagtcca
      721 tccggggttg gatgcggcgg cggcacgggt gggaggtccg gggtgagtgg atcgcctctg
      781 ctcccggtgt ggtctgcgcg gtcaacctgc tcatccggac gctgaccaac cggggcgacg
      841 gggtggtact ccagccacca gtctactatc ccttcttccg tggcgtggag gagaatggct
      901 gccgcgtgct ctccaacccg ctccggtacg atggaacccg ctacgtcatg gattttgaag
      961 acctcgagcg gaagctcgac caggcaaagc tcctcgtcct gtgcagcccc cataaccccg
     1021 ttggccgggt ctggacgccg gaggagctgc tccggctggg ccagttgtgc ctcgcgcggg
     1081 gtgtggtcat catctccgat gagatccact gtgatctgac gttccccggc catccgcaca
     1141 cggccttccc cacgctgtcg gacgccctgg cccagcactc catcgtgtgc acgtcaccca
     1201 gcaagacctt caaccttccc ggcctgcaac ccggaatcat cgtcatcccg aacccgaggc
     1261 tccgccgaga cttcctgcga accctgcgcg cctgcggcat cccggatacg aacgtcttct
     1321 gcctggaggc ggtcaaggcc gcctataacc acggcgagcc gtggttgaag gacctcctgg
     1381 cctacctgga ggagaatcac aacctgctcc ggcggttcgt cgccaaggag atccctcggc
     1441 ttcgcgtggt gccgtccgag gggacgtacc tggcctggct ggactgtcga ggtctgggtc
     1501 tggaacccaa gaccctggaa cagctcctgc tgatcaaagc cagactcgtc ctgaatcaag
     1561 gctatacttt cggtgtggga ggggagggct tcgtgcgtat gaaccttgcc tgcccgcggg
     1621 ccatgctgga agatggactc ggccgtcttg cctcggccat cgcgggcgtc ccgtgatcgg
     1681 gagagggcgg caccggttcc gtggatggtc cggctgaacc gccggcttgt ccgcgatgaa
     1741 tgagcgtgta acagcagacc aaggtccgga tagagaattc ccaaggaagg agcgttcgca
     1801 tgaaacccgt cgtgctggtc acccgcaaac tgcctgacgc cgtcgaggat cgcctccggc
     1861 gcgattatca gccacggctc aatcccgacg accgcctcta ttccagcgag gagcttatcg
     1921 cgaaggccgg cggcgccgcg gcgatcgttc cttgtcacac ggaacgtttt tcggccgagg
     1981 tcatccagcg ccttcccgat accgtccgca tcattgccaa tttctccgtg ggctacgacc
     2041 atgtcgatct cgaggcggcg aaggcccggg gcatcatcgt caccaacacg cccgaagtcc
     2101 tgtccgatgc caccgccgag ctgaccatcc tgctcatgct gggcgcggcc cggcgggccg
     2161 gcgagggaga gcgcctggtg cgaacccgag agtggcggga ctggagcccc agtttcatgg
     2221 tgggcaccca gatgaccggc aagcgtctcg gcatcgtggg catgggacgg gtcggtcgcg
     2281 tcaccgctcg gagggctcgg gggttcgaca tggtgatcca ctaccacgat atccgtcgcc
     2341 tccctccgga gcaggaggag ggcgcgatat ttcacccgac cccggaagac ctcttgccgt
     2401 actgcgactt cctggcgttc cactgcgtcg ccacgcccca gacccggaag ctgctcaatg
     2461 ccgagcggat cgccctgctc ccacagggcg ccatcgtcgt caacgccagt cgtggggtag
     2521 tcgtcgacga cgaggcattg atcgcagcgc tgaagtccgg cagggtggcc gcggccggcc
     2581 tcgacgttta caacaacgag cccaacatcc atccggggta ccgggagcta ccgaatacct
     2641 ttctgatgcc gcacatcggc agcgccacca aggagacccg cgacggcatg ggattccggg
     2701 cgctggacaa tctggatgcc tttttcgccg gccgcggacc ccgtgatcgc gtggcctgag
     2761 gccacagggt cacggatgca gggacacggt tcgagattgg aagatggcgg caggtatgcc
     2821 ggtgccgatc tttcagcttg atgaacagtg gagcgggcgg ggcggcgcag gctgagtcga
     2881 cccgccgctg ggggcgagca tggggcaacg aatcggagca ttcctcatcc ctgggatgga
     2941 tttccgcccg atgctgacgt tggcgcacga agcggagggc tggggctacg agagcctgtg
     3001 ggttacccac gggggcggtc gggacgcctt cctagtcctg tcggcgtacg cccacgtgac
     3061 ccggaccgtt gggctcggga ccggtgtggt cccgat
//
LOCUS       DBNGZC010000146         2383 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2359694, whole genome shotgun sequence.
ACCESSION   DBNGZC010000146 DBNGZC010000000
VERSION     DBNGZC010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2359694"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1632
                     /locus_tag="AC16G0_02845"
     CDS             <1..1632
                     /locus_tag="AC16G0_02845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HIR5098589.1"
                     /translation="VDPLPIPPVMTPVIANLNPAPTKGPNPDPAVRVPTPPTVVDVTG
                     FTETNRPDHQRWEEFPPVEMYESVEQAVKAIFYPLVDGVPPSTVWTFVERSTGAVGPL
                     RIKAQYGRPILHRIHNALPEDNGGFGKNVTTTHLHNGHNASESDGGPLQGYVAGRFKD
                     FHWPNVRAGFSKTHPTTTLNGRTVRGDFRETMSFLWFHDHEHDFTAQNTYKGLASFYT
                     LFSDDILLDTLDETTAVGLHLPSGEFDIPMLFQDKVFDPHTGQLFFDLFNLDGILGDK
                     VAVNGKIQPFLEVKQRKYRFRFLVGGPSRVFEFFLSNGQPFIQLSNDGNLLPRPLSRK
                     SIRQQVAERVDVIVDFSQATPGDKIYLQNRLEQVDGRKPTGNLIAPTNVVEFRVVPLA
                     PGEKDNSQVPATLLALPSTNVAIAQRRSFDFDRQGGAWAVNGQFFNEQISAFVKQNTA
                     EIWTFNSGGGWVHPAHPHSEEFQILSRDGAPPPVFEIARKDMAGVGAAVLGTQDSSEV
                     KIFMQFRDWQGDYPMHCHNTVHEDHGMMILFEIVP"
     gene            1648..2256
                     /locus_tag="AC16G0_02850"
     CDS             1648..2256
                     /locus_tag="AC16G0_02850"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR5098590.1"
                     /translation="MEKRFIQISAAERRRNRFRNVTLTTHEGKAVKFYDDLLKDKTVL
                     LSFFYTNCVAEAICPLTTANLVEVQKLLGPQVGRDIFMYSITLDPEHDTPQVLQEYAR
                     AFQVKPGWLFLTGAKADIEVLRRNLGYVDLDPVKDRDRSQHIGMVRYGIEPLERWAGC
                     PSLSKPRTIVDFLTRLDPTREWPGVGDPDVHPEPALAHDHHS"
BASE COUNT          472 a          786 c          708 g          417 t
ORIGIN      
        1 gttgatcccc tccccatccc gcccgtcatg acgcctgtca tcgctaatct gaatccagcc
       61 cctaccaagg gcccgaaccc ggatccagcc gtgcgcgtgc caacgccccc aactgttgta
      121 gatgtcacgg gcttcacgga aaccaaccgg cccgaccacc agcgctggga ggagttcccc
      181 cctgttgaga tgtacgagag cgtagagcag gcggtcaagg ctatcttcta ccccctcgtc
      241 gacggggtgc cgccatccac agtctggaca ttcgtggaac ggtccacggg ggcggtgggt
      301 ccgctgcgga tcaaggccca atatgggcgt ccaatcctcc accggatcca caacgccctc
      361 ccggaggata acggggggtt cgggaagaat gtgacgacta ctcacctcca caacgggcac
      421 aacgcctccg agtcggacgg ggggccgctc caggggtatg tggcggggcg cttcaaggac
      481 ttccactggc ccaacgtccg ggccggcttt tcaaaaaccc atccgacaac caccttgaac
      541 gggcgcaccg tccgcgggga ttttcgggag accatgagtt tcctctggtt ccacgaccac
      601 gagcacgact tcaccgcgca gaacacctac aaggggctcg cctccttcta caccctgttc
      661 agtgacgaca tcctgctgga caccctcgat gaaaccaccg cggttggtct ccatctcccc
      721 agcggggagt ttgacatccc catgctcttt caggacaagg tcttcgaccc ccacaccggg
      781 caactcttct tcgacctctt caacctggat ggcatcctgg gggacaaggt cgcggtgaac
      841 gggaagatcc aacccttcct ggaggtcaag cagcggaagt accgtttccg ctttctggtg
      901 ggcgggccgt cccgggtctt cgagttcttt ctgagcaacg gtcaaccctt catccagctc
      961 tccaacgatg gaaacctcct tccccgcccc ctgtcccgga agagcatccg gcagcaggtg
     1021 gcggagcgag tggatgtgat cgtggacttc tcccaggcca cgcccgggga caagatctat
     1081 ctccagaaca ggctcgagca ggtggacggc cgaaagccca cggggaacct cattgccccg
     1141 accaacgtcg tcgagttccg ggtcgtgccc ctggcgcccg gcgaaaaaga taacagccag
     1201 gtgccggcga cactcctcgc cctgcccagc acgaatgtcg caatcgcaca gcgccggtcg
     1261 tttgatttcg atcggcaggg gggcgcctgg gcggtgaacg ggcagttctt caacgagcag
     1321 atcagcgcat tcgtcaagca gaacacggcc gagatctgga ccttcaattc gggcgggggc
     1381 tgggtccatc cggcccatcc tcacagtgag gaattccaga tcctgagccg ggacggtgca
     1441 ccgccgcctg ttttcgagat tgcccgcaag gacatggccg gggtgggggc cgccgtcctc
     1501 ggcacccagg actcctccga ggtcaagatc ttcatgcagt tccgcgactg gcagggagac
     1561 tacccgatgc actgccacaa caccgtgcac gaggaccacg gcatgatgat cctgttcgag
     1621 atcgtgccgt aaggaaaggg gcgcgccatg gagaaaaggt ttatccagat ctccgccgcc
     1681 gagcggcggc ggaaccggtt ccgcaacgtc accctgacga cccacgaggg gaaagcggtc
     1741 aagttctacg acgacctgct caaggacaag accgtcctac tcagtttctt ctacaccaac
     1801 tgcgtggcag aggccatctg tcccctgacc acggcgaacc tcgtggaggt ccagaagctc
     1861 ctggggcccc aggtcggccg ggacatcttc atgtactcca tcaccctgga cccggagcat
     1921 gatacccccc aggtcctcca ggagtacgcc cgagcgttcc aggtaaagcc gggctggctg
     1981 tttctcacgg gggcgaaggc agacatcgag gtcttgcggc gcaacctggg ctacgtggac
     2041 ctcgatcccg tcaaggaccg ggaccggtcg cagcacatcg ggatggtccg ctacgggatt
     2101 gagcctctgg agcggtgggc tggctgtccg agcctctcca aacccagaac catcgtggac
     2161 ttcctcaccc ggcttgatcc caccagggag tggccgggag tcggagaccc ggacgtccac
     2221 cctgagccgg cgttagctca cgatcaccac agctgaggat atgaaagatt ggcggggcag
     2281 aaccggatcc atcaaggggc ttcgcctggc ggatgaccca tctgttcctt gctgcggtca
     2341 cgtctatctc gaactgcaac gacgcgacgg gcgggcggtg atg
//
LOCUS       DBNGZC010000147         3037 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_12551, whole genome shotgun sequence.
ACCESSION   DBNGZC010000147 DBNGZC010000000
VERSION     DBNGZC010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3037)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_12551"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2273
                     /gene="mfd"
                     /locus_tag="AC16G0_02855"
     CDS             <1..2273
                     /gene="mfd"
                     /locus_tag="AC16G0_02855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR5098591.1"
                     /translation="ILVARSDAQAQRLQEILRDHDLGARRGRDLPEPGGIALWTGSLS
                     AGFRIPPAGLTFITEAEIFGARALPRRRAHPKEALPFSSFEDLKAGDYVVHVDHGIAR
                     YLGLQQLSAGGQAGDFLHLQYAGTDTLYVPVGKRHLVQRYVAGESAGARPALDRLGGT
                     SWAKAKERVRASVRETAEELLKLYAAREVLPGHVFAPDTPWQKEFEAAFPSAETPDQL
                     QAIRDVTRDMEAPRPMDRLVCGDVGYGKTEVALRAAFKAVMDGRQVAVLVPTTVLALQ
                     HHQTFSARFANFPVEVEMLSRFRPDAEQRQVLRSLAEGGVDILIGTHRLLQKDVKFAA
                     LGLLVVDEEQRFGVAAKEALKQLRREVDVLTLTATPIPRTLHMTMLGVRDISTIETPP
                     EERLAIRTYVTPYDPQVITEAIQREVARGGQVFFVHNRVESIHAVARRLKQLVPEARL
                     AVAHGQQSEAALEKVMVDFYMKKVDVLLCTTIIESGLDVPSANTIIIDRADTLGLAQL
                     YQLRGRVGRDKYRAYAYLLIPAEGGMTEVARKRLQVIAELTELGSGFKIASRDLEIRG
                     AGNLLGAEQSGHIAAVGFDLYTQLIQETVRELKGQPLETVVDPTIRLHVEGFIPEPYV
                     PDATLRLNLYKRLSATTEADRLPIFAEELADRFGPLPGEVERLLRVMELKIEARDLRI
                     KEIDARREAIRVSFAPDPPVSPQTILALLRAERGRLKYVPEDTLEYRTDAPTPEARIE
                     AARKLLQRLRTGVTVH"
     gene            2478..>3037
                     /locus_tag="AC16G0_02860"
     CDS             2478..>3037
                     /locus_tag="AC16G0_02860"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HIR5098592.1"
                     /translation="MKKTMGLWSGLVVAAGMALVVAGCGRGGGDTKAQGPAVLKINDK
                     SFTAADVEKDIAQEVRRSPRELGPFLATKDGQKQFLDRIVRRELLLQEAEKRKIGDQP
                     QIVDQVTALRRELMIRTLLQEEIGAKIKVEDKEVQDYYTAHPEEFSGDKVRARHILVQ
                     TEDEAKQVLDRLGKNEPFEELAKTLS"
BASE COUNT          517 a         1032 c         1034 g          454 t
ORIGIN      
        1 gcatcctggt ggcgcggagc gacgcccaag cccagcgcct gcaggagatc ctgcgggatc
       61 acgacctggg ggcacgccgg gggcgggatc tccccgagcc gggcgggatc gccctctgga
      121 cggggagcct ctccgccggt ttccggatcc cgccggccgg cctcacgttc atcaccgagg
      181 ccgagatctt cggcgcccgg gccctgccgc ggcggcgcgc gcacccgaaa gaggccctgc
      241 ccttctcgtc cttcgaagac ctgaaggccg gagactacgt ggtgcacgtg gaccatggta
      301 tcgcccgcta cctcggcctg cagcagctct cggcgggcgg gcaggcaggc gactttctcc
      361 acctccagta cgccgggacc gacaccctct acgttccggt cgggaagcgc cacctggtcc
      421 agcgctacgt cgccggggag tccgccggcg cccggccggc cctggaccgg ctgggcggga
      481 cctcgtgggc caaggccaag gagcgcgtcc gcgccagcgt ccgtgagacg gccgaggagc
      541 tgctcaagct gtatgccgcc cgcgaggtcc tgccgggcca cgtttttgcc cccgacaccc
      601 cctggcagaa ggagttcgag gcggccttcc cttccgcgga gacccccgat cagctccagg
      661 ccatccggga cgtgacacgc gacatggagg ccccgcggcc catggatcga ctcgtctgcg
      721 gggacgtggg ctacgggaag accgaggtgg ctctgcgcgc ggccttcaag gcggtcatgg
      781 atggcaggca ggtcgccgtc ctcgtgccca ccacggtcct ggccctccag caccaccaga
      841 ctttcagcgc ccgcttcgcc aactttccgg tcgaggtgga gatgctttcc cgcttccggc
      901 ccgacgcgga gcaacgccaa gtcctgcgca gcctcgccga ggggggcgtg gacatcctca
      961 tcgggaccca ccggctgctc cagaaggatg tgaagttcgc ggccctgggg ctgctcgtcg
     1021 tggacgagga gcagcgcttc ggcgtggccg ccaaggaggc cctcaagcag ttgcggcgcg
     1081 aggtggacgt cctcaccctc acggccacac ccatcccccg gaccctccac atgaccatgc
     1141 tgggcgtgcg ggacatcagc accattgaaa cccctcccga ggagcggctg gccatccgga
     1201 cctacgtcac cccttacgat ccccaggtca tcaccgaggc catccagcgg gaggtcgccc
     1261 gcggcggcca ggtgttcttc gtccacaacc gggtggagag catccacgcc gtggcccgcc
     1321 gcctgaagca gctcgtgccc gaggcgcggc tcgccgtggc ccacggccag cagagcgagg
     1381 cggccctgga gaaggtcatg gtggacttct acatgaagaa ggtggatgtc ctcctctgca
     1441 ccaccatcat cgagtccggt ctggacgtcc cctcggccaa caccatcatc atcgaccgtg
     1501 cggacaccct ggggttggcc caactctacc agctccgggg ccgggtgggg cgggacaagt
     1561 accgggccta cgcctatctc ttgatccctg cggagggcgg gatgaccgag gtggcacgga
     1621 aaagactgca ggtcatcgcc gagctgaccg agctgggctc cggcttcaag atcgcctccc
     1681 gggacttgga gatccggggg gccgggaacc tcctgggggc ggagcagtcg gggcatatcg
     1741 ccgcggtggg cttcgatctc tacacccagc tcatccagga gaccgtccgc gagctgaagg
     1801 ggcagcccct ggagaccgtg gtggacccca ccatccgcct gcacgtcgag ggcttcatcc
     1861 ccgagcccta cgtccccgac gccaccctgc gcctgaacct gtacaagcga ctcagcgcca
     1921 cgaccgaggc cgaccggctc ccgatcttcg ccgaggaact cgccgaccgg ttcggtccgc
     1981 tccccggcga ggtggagagg ctgctccggg tgatggagct caagatcgag gctcgggacc
     2041 tcaggatcaa ggagatcgat gcccgtcgcg aggccatccg ggtctccttc gccccggatc
     2101 ccccggtgag tccgcagacg atcctggcgc tgcttcgggc ggagcggggc cgtctgaagt
     2161 acgttcccga agacacgctg gaataccgga ccgatgcccc cacccccgag gcgcggatcg
     2221 aggccgccag aaaactcttg caaaggctcc ggaccggtgt tacggtccat tgactttctc
     2281 ccttgtgggg agggggtcgg gcagccctca gcattcagcc cccgattgtc ggattgacac
     2341 accagagctg cgattcccgt gattccctgc gcggactatt gctgatgggt gactgcgtct
     2401 tgagcgcttc cggggcgttc gtccgccgga cggaagcggg tgatccgggc cgagccgatg
     2461 gagggaagga gccgagcgtg aagaaaacga tggggctgtg gagtgggttg gtcgtggccg
     2521 ccggtatggc gctggtggtg gccgggtgcg ggagaggcgg cggcgacacc aaggcccagg
     2581 gcccggcggt cctcaagatc aacgacaaga gcttcaccgc ggcggacgtg gagaaggaca
     2641 ttgcccaaga ggtgcggcgg tctccgcggg aactcggacc ctttctggcc accaaggacg
     2701 gccagaagca attcctggat cggatcgtcc gccgcgagct cttgctgcag gaggcggaga
     2761 agcggaagat cggcgaccaa ccccagatcg tggaccaggt gacggccctc cggcgggagt
     2821 tgatgatccg gaccctcctc caggaagaga tcggggccaa gatcaaggtg gaggacaagg
     2881 aggtccagga ctattacacg gcccacccgg aggagttttc aggcgacaag gtccgcgcgc
     2941 ggcacatcct ggtgcaaacc gaggacgagg ccaagcaggt cctggacaga ctcggcaaga
     3001 atgagccctt cgaggagttg gccaagaccc tgtccaa
//
LOCUS       DBNGZC010000148         3978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2556178, whole genome shotgun sequence.
ACCESSION   DBNGZC010000148 DBNGZC010000000
VERSION     DBNGZC010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2556178"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..887
                     /locus_tag="AC16G0_02865"
     CDS             126..887
                     /locus_tag="AC16G0_02865"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5098593.1"
                     /translation="MKLKGKVALVTGGGTGMGRAISLKFASEGADLIVNYSRRAKEAG
                     ETAEAAGKLGVRAFAIQADVSQDNEARRLVEEAVKRLGRLDILVNNAGWSQVVPHRDL
                     EKLTEEVLERTWAVNTKGPLYLCRAAIPHMLKVGGGAIVNITSIAAFQGAGSSVIYCA
                     SKAALGAITKSLARAFAKDNIRVNSIAPGFVDTNFVDWPREVMEKNTKVSPMGRIPTV
                     DDIANTALYLACDATGVTAQTVLVDCGVTVLQPVV"
     gene            1150..1938
                     /locus_tag="AC16G0_02870"
     CDS             1150..1938
                     /locus_tag="AC16G0_02870"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR5098594.1"
                     /translation="MTREGFVQTVSNKAFHAVRKTRVSKEIIDQVRDLISSGRLEPGD
                     RLPSERELAQTFRVGRSTVREAIRSMESLGLIAVRPGEGTFLAGPSARSAQDPLTANL
                     FKAWSTHFKLFEVRAVLEPSLAALAARRATPEQIEAMRSILADQEREILRGETGQKAD
                     SAFHHLIAEATGNEILHNVAESLMTLLGETRETSLQHSRRPSSSLKQHRAILAAIESR
                     NSAAAERRMREHIRSIERLIFSSHQKPAEATEGAATSATPGVSS"
     gene            1935..>3978
                     /locus_tag="AC16G0_02875"
     CDS             1935..>3978
                     /locus_tag="AC16G0_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5098595.1"
                     /translation="MTEAAGQEGLQFKVAEAFVEDVGKGLARLDADDLMRLHAAPGDV
                     LLIKGRRSTVARAAQAPPSHCGQLLILMDGTTRDNAQAGVDEYVTVRKVPFKAAESLL
                     LTPVQAGQSAPTEEEIPHLRQLLLGLPVILGDHLQITFLGSRPRSFTLDGAAPRGALL
                     ISTDTAITFKVPEVSAERAYRVSYEDIGGLDREVSLVREMVELPLKLPQIFGLLGIDP
                     PKGVLLTGSPGTGKTLIARAVSNEVRAHFIHVNGPEIIHKFYGESEAKLRQVFEEARR
                     NAPSIIFLDEIDALAPKRAMVVGDVEKRVVAQLLALMDGLVERGEVVVIGATNMPDLV
                     DPALRRPGRFDREITIGVPGREGRRQILGIHSRRMPLGPDVDLDQLAEITHGYVGADL
                     AVLCKEAGMVALRRLVPQVRFDVDLKPQMDALAVQITREDFMSAFKVVEPTSTREFLA
                     ERPRLTLKDVGGLAETKRTLASILQLFRGGAALFAHTRFNPPKGILLTGPSGTGKTLL
                     ARSLAGELGLTLITVDQPSLLSKWLGESEKGLREVFKRAKQASPCILFFDEIETMVPA
                     RSIQEVGSVFPRLVSQLFRELDDLHGSLGVVVLAATNRPDLMEPALLRAGRFDYVLEL
                     PLPTRDERREILAIHTEGLPLEDDANLGEIADATGGWTGADLELICKKATILALEEF"
BASE COUNT          744 a         1244 c         1272 g          718 t
ORIGIN      
        1 agcctggatg acgcctgctg cgaccgcagc cccccagccg acgaaaagag cttgacccct
       61 tggaccgact tcgcgaagat aagcggcgtt cgttcacttc tcaacctcat tgacaggagc
      121 gagtgatgaa gctgaaaggc aaggttgcgc tggtaacagg tgggggcacg ggtatgggta
      181 gggcgatctc tctgaagttt gcctcggagg gagcggacct gatcgtcaat tactccaggc
      241 gggcgaagga agccggggag accgccgagg cggccgggaa gcttggtgta cgcgccttcg
      301 ccatccaggc ggatgtgtct caggacaatg aggcgcgccg actcgttgag gaagcggtca
      361 agcgcctcgg ccggttggat atcctggtga ataacgcggg ctggagtcag gtggtgccac
      421 accgcgatct cgagaagttg acagaagagg tcctggagcg gacctgggcc gtgaacacca
      481 agggtccgct gtacctctgc cgggccgcga tcccgcacat gctgaaggtc ggcggcggcg
      541 ccatcgtcaa catcacctcc atcgccgcgt tccagggtgc cgggagttcc gtgatctact
      601 gcgcctccaa ggcggccctt ggagccatca cgaaatccct ggcccgtgcc ttcgccaagg
      661 ataacattcg ggtcaattcc attgcgcccg gttttgtgga caccaacttc gtggattggc
      721 ctcgggaggt catggagaaa aataccaagg tcagcccgat gggtcgcatc cccactgtgg
      781 acgacatcgc gaacaccgcc ctctatttgg cctgcgacgc gacaggggtc accgcccaga
      841 cggtcctcgt ggactgcggc gtgaccgtcc tccagcccgt cgtgtaaccg ccagtttatc
      901 atcgtcagct cctcgcaagg ctgccctcga ttggcacatc gagcgcagcc tgatatgaat
      961 ttcaatccgg actgcttcta tcttctaact tccaataaaa taagggcttg acggcatcgg
     1021 gagtgcgtac cagattatgg tcgtggtccg accaccgggc catccggcca tcacacccat
     1081 tccgaccaga acagtcccaa ttaagggaat gctgtctgac cgggctgcta ttttcattta
     1141 gagaattacg tgacgcgaga gggattcgtg cagacagtct ccaataaggc ctttcacgcc
     1201 gtccgcaaga cccgggtgtc aaaggaaatc atcgatcaag tccgcgacct catctcctcc
     1261 ggtcgcctcg aacctggaga tcggctgccc tccgagcgcg aactcgccca gactttccgc
     1321 gttggacgct ccaccgtgcg cgaggccatc cgctccatgg agtccctcgg actcatcgcg
     1381 gtccgaccgg gtgagggaac gttcctggct ggtccctcag cacggagcgc ccaggacccg
     1441 ctcactgcca atctgttcaa ggcctggagc acgcacttca agctgttcga ggtgcgggcc
     1501 gtgctcgagc cctcgctggc cgccttggct gcgcggcgag ccacgccgga acagatcgag
     1561 gccatgcgaa gcatcctggc cgaccaagag cgcgagattc tccgtggaga gaccggccag
     1621 aaagcggaca gcgcgtttca ccatctgatc gccgaggcga ccggcaacga gattctccac
     1681 aatgtcgccg agagcctgat gaccctgttg ggggagactc gagagacttc actgcaacac
     1741 agccgccgcc cgagcagctc tctgaagcaa catcgggcca tcctcgcggc gattgaaagc
     1801 cggaattcgg ccgcggccga gcgtcggatg cgcgaacaca tccgcagcat cgagcgcttg
     1861 atcttctcat cccaccagaa accagcagag gcaacggaag gggccgcgac ctctgcgacg
     1921 ccaggggtgt cctcgtgacc gaggcggcgg ggcaagaggg actgcagttc aaggtggcag
     1981 aggcgttcgt ggaggacgtg ggcaaaggtc tggcccgcct ggatgcggac gatctgatgc
     2041 gtctccacgc ggcacccggg gacgtcctgc tgatcaaagg tcgtcgatcc acggtggctc
     2101 gggcggcgca ggcgcctccc agtcattgcg gacaactgct gatcttgatg gatggaacaa
     2161 cgcgggacaa cgctcaagcg ggtgtggacg agtatgtcac cgtccggaag gtgcctttca
     2221 aggcagccga gtctctgctc ctgacgccag tccaagccgg gcagtccgcc cccaccgaag
     2281 aggagatccc gcacctccgc caactccttc tgggtctgcc ggtgattctc ggcgatcacc
     2341 tgcagatcac cttcctgggc tcgcgacctc gctccttcac gctggatggc gcggcaccgc
     2401 gaggggcgct gctcatctcc acggacacgg ccatcacctt caaggtcccc gaggtctccg
     2461 cggagagagc ctaccgtgtc tcctacgagg acattggcgg cctggatcgg gaagtgtcgc
     2521 tggtccggga gatggtcgag ctgccgctga agctgcctca gatcttcggt ctcctgggta
     2581 tcgatccacc caaaggtgtg ttgctcacgg gctcgcccgg cacgggcaag accctcatcg
     2641 cccgcgcggt gtccaacgag gtccgagcgc acttcatcca cgtcaacggg ccggagatca
     2701 tccacaagtt ctacggcgag agcgaggcca aactgcgcca ggtgttcgag gaggcgcggc
     2761 ggaatgctcc cagcatcatc ttcctggatg agatcgacgc cttggccccc aagcgtgcca
     2821 tggtcgtcgg ggacgtggaa aagcgggtgg tggcccagct cctggccctg atggatggcc
     2881 tggtggaacg cggagaggtg gtggtcatcg gggccaccaa catgcccgac ctggtggatc
     2941 cggcgctgcg gcggccgggc agattcgatc gggagatcac catcggcgtc cccggtcggg
     3001 aaggtcggcg gcagattctg gggatccatt cgcgccggat gcccctgggc ccggacgtag
     3061 acctggacca gctcgcggag atcacccacg gctatgtcgg cgcagacctg gccgttcttt
     3121 gcaaagaggc gggcatggtt gcccttcgtc ggctggtccc gcaggtgcgc ttcgatgtgg
     3181 acctgaaacc acaaatggac gccttggcag tgcagatcac ccgcgaggac ttcatgtcgg
     3241 ccttcaaggt ggtggagccg acctcgactc gggaattcct ggcggaacgt ccacgactca
     3301 ccctgaagga cgtgggtggc ctcgccgaga ccaagcggac ccttgcatcc atcctgcagc
     3361 ttttccgcgg gggagccgcc ctgtttgccc atacccgttt caatccgccc aagggaatct
     3421 tgctcaccgg cccatctggg accggcaaga cgctgctggc acgctcgctg gcgggcgagt
     3481 tggggctcac cctgatcacg gtggatcagc cgagcctcct gtccaagtgg ctgggcgagt
     3541 cggagaaggg actccgcgag gtgttcaagc gggccaagca agcctccccc tgcatccttt
     3601 tctttgacga gatcgagacg atggttccgg cacgctccat ccaggaagtg gggagcgtgt
     3661 tcccacgcct ggtgagtcag ctcttccgcg aactggacga tctgcacggc tcgctgggtg
     3721 tggtggtcct ggcggcgacg aatcggccgg acctcatgga gcctgcgctt cttcgggccg
     3781 gccggttcga ctatgtgctg gagctgcccc tccccacccg ggacgagcgg cgggaaattc
     3841 tggcgattca caccgaggga cttcctttgg aggacgatgc aaatctcggc gagatcgccg
     3901 acgctacggg gggttggacg ggcgcggatc tggaactgat ctgcaagaag gcgacgatcc
     3961 tggcgctgga agagtttc
//
LOCUS       DBNGZC010000149         1465 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1773195, whole genome shotgun sequence.
ACCESSION   DBNGZC010000149 DBNGZC010000000
VERSION     DBNGZC010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1465)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1773195"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..299)
                     /locus_tag="AC16G0_02880"
     CDS             complement(69..299)
                     /locus_tag="AC16G0_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098596.1"
                     /translation="MPVRPESRDTVPASSPPEAHSGSVSRETSYRYDSPPSRIATLNI
                     GNVLDVGVSLRSHRRLVGTLWRIRWTVPLTAT"
     gene            complement(1033..>1465)
                     /locus_tag="AC16G0_02885"
     CDS             complement(1033..>1465)
                     /locus_tag="AC16G0_02885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5098597.1"
                     /translation="TVIEQEYRPGQATSRQPTVLCIDDDRLILGLCREALQRRGYRVL
                     LAADGRTGIEEARRERPALILLDFMMPTMDGLEVCRRLRAEPALRETRIILLTASERP
                     DLDVEARAAGATLTLRKPFGPDAIVATVDGVLGRTLGPGPP"
BASE COUNT          256 a          459 c          467 g          283 t
ORIGIN      
        1 gttcctcccc ctttctgctg ccacgacgtc cccagcgccc gtcgcctgtt ggcaggtcca
       61 gccgggcgct acgtggccgt cagcggaacg gtccagcgga tccgccagag ggtccccaca
      121 aggcggcggt gactccgcag actcaccccc acgtccagca cgttcccgat gttcagcgtc
      181 gcaatccgcg agggcggcga atcgtaccgg taggaggtct cgcgggagac ggaacctgaa
      241 tgcgcttccg gaggcgacga ggccgggacg gtgtcgcggg actcgggtct caccggcatg
      301 gtggtgctct gagcgtgaga aggccaattg cggttagacg gcagcatcct gcgtgctccg
      361 cgtagcaggt atcacggatg aaaaatccag tcaagcataa actcaacttg tgaaaaatat
      421 tttcagtcct gtcaatggtc ccccagggga tggtggcacc gaaagaacct gcgttcaggg
      481 ggtccccgac gggtggttga gaatctgacc cactgtccgc aactcctcca ttcgctggat
      541 cggctgtaat ggcgtttcgc acacgtcggt gaggggaaac ccttttccct gcccaccgga
      601 tgagattttg gcgaaggata gacgcggagt ggcgaccacc gcgatccgaa aggcaaatcg
      661 gggtgcgcgg gatctttcgc tcatgaatca atggcagcag gcgtcattga aagaacaggg
      721 gcctgagctt ccttcgctca gacccctgtt ttcgagatgc tatctggttg gggcgactgg
      781 atttgagcca gccacccctt ggtcccgaac tgtccgaggt ttcttcgtcc gtgttccctg
      841 ccagtctgcg gtgtcccgcc tcatccatgt atcttcagca cttacgcggt aacccttacg
      901 ggtgacttta gctggcgttc gcccctggtc cggggtgtac cggcctcccc tcgtggaact
      961 agagtcacca agtcgggtgt ggtggagtca ttggcgaatc gaaaccgcct tcattggaac
     1021 tcgtcgtccc cctcacgggg ggccggggcc gagcgtccga cctaggacac catcgacggt
     1081 cgccacgatg gcgtccggcc cgaagggttt ccgcagggtg agggtcgccc cggccgctcg
     1141 cgcctcgacg tccaggtcgg gcctctcgct ggccgtcaag aggatgatcc gggtttcccg
     1201 taaggcgggc tcggctcgga ggcgccggca gacctcgaga ccgtccatcg tgggcatcat
     1261 gaagtccaga aggatgagcg ccggccgctc gcgcctggcc tcctcgatcc cggtacgtcc
     1321 atcggcggcc aggaggaccc ggtagcctcg tcgttgaaga gcctcgcggc acaaccccaa
     1381 gatgagccgg tcgtcgtcaa tgcagaggac cgtgggttgc cgggaggtcg cctgccctgg
     1441 ccgatactcc tgctcaatca cagtg
//
LOCUS       DBNGZC010000150         2172 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_990593, whole genome shotgun sequence.
ACCESSION   DBNGZC010000150 DBNGZC010000000
VERSION     DBNGZC010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_990593"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="AC16G0_02890"
     CDS             complement(<1..843)
                     /locus_tag="AC16G0_02890"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5098598.1"
                     /translation="MTTEGVSLKEAQRITSFPRTALRLLILALLLLPLAGGQYAQYLA
                     IQVMILSLFALGFNILFGYSGLLSFGQAGFYAVGAYGCAKLLLMKPSLLFGVIGGTLL
                     AGVAALVLGVLCIRHTRIYFSMLTLAFGMMVYSVALNWRDFTGGDDGLVGVPRAALEI
                     PGILSLNLATMGRYYYFVLVTTLLAVWLAYRLVHSPLGLTFQGIRDSETRMAFTGVPV
                     RRYRLIAFVIAGLYAGLAGALLPPLESTVTPPVAHWTTSADPVLATLLGGIQTFAGPI
                     VGAFL"
     gene            complement(847..1704)
                     /locus_tag="AC16G0_02895"
     CDS             complement(847..1704)
                     /locus_tag="AC16G0_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5098599.1"
                     /translation="MQTLAFQGLIGLSLAMYLWLISAGLTIIFGVLGVINFAHGSLFM
                     LGAYFAFTFYGLLGWNFFLAIALSLLATAIVGLVMERFFLRYLYGVDEAYQLLLTFGF
                     VLILDDAVKMIWGGVFRIPPIPGFLEGAWSILGRPFPVYNLFIIAFGVAVALILWLVF
                     ERTWWGKIVRATASDREMANAIGVNVPGVFSGVFMLGAMLAALGGALGTPLRVASPGL
                     GAAMIIQAFVITVIGGLGNLKGAFVGSLIVGILSAYGTLLFPVFELALIYMVMAVVLL
                     VRPQGLFAR"
     gene            complement(1854..>2172)
                     /locus_tag="AC16G0_02900"
     CDS             complement(1854..>2172)
                     /locus_tag="AC16G0_02900"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098600.1"
                     /translation="KRWSRFPNYSAETTYSAVMIAKAAIEKARSLDSGKMIGALNGMQ
                     INTPAGLRVFRSEDHQFVYNVPAGRVMMDPKYPIPVLGDLKVLPVKDYYRWPPFTPIS
                     ASK"
BASE COUNT          477 a          674 c          709 g          312 t
ORIGIN      
        1 gaggaaggcc cccacgatcg gcccggcgaa cgtctggatt cctcccagca gtgtcgccag
       61 aaccggatct gcggacgtgg tccagtgggc gacgggaggc gtgaccgtgc tctccagagg
      121 cggcaagagc gcgccggcca gccccgcgta cagcccggcg atcacgaagg cgatgaggcg
      181 gtagcggcgg acgggcaccc ccgtgaaggc catgcgcgtc tcgctgtctc ggataccctg
      241 gaaggtcagc cccagcggag aatgcaccag ccggtacgcg agccaaacgg ccaggagcgt
      301 cgtcaccagg acgaagtagt agtatcgccc catggtggca aggttgaggg agagaatccc
      361 gggaatctcc agggccgccc gggggacccc gaccagtccg tcatccccgc ccgtgaagtc
      421 ccgccagttc agtgccacgg agtacaccat cattccgaag gccagggtca gcatggagaa
      481 gtaaatccgg gtgtgccgga tgcagagcac ccccaggacc agcgccgcca cccctgccag
      541 cagggtgccc ccgatcacac cgaacaagag ggagggcttc atcagaagga gcttggcgca
      601 gccgtatgcc cccaccgcat agaagcctgc ctggccgaag gacagcagcc ccgaatagcc
      661 gaagaggatg ttgaagccca gggcgaagag ggacaggatc atgacctgga tggccaggta
      721 ctgggcgtac tgtccccccg ccaggggaag gaggagcagg gcgaggatga gcagcctcag
      781 tgccgtacgg ggaaacgacg tgatcctttg agcttccttc agagagacac cctcggtcgt
      841 cacggctcac ctcgcgaaca ggccttgggg ccggaccaac aggaccaccg ccatgaccat
      901 gtagatcagg gccagctcga agaccgggaa aaggagcgtc ccgtaggcgc tgaggatgcc
      961 cacgatcagc gatcccacga aggcgccctt caggttcccc aggccgccga tgacggtgat
     1021 gacgaaggcc tggatgatca tggctgcccc caggccgggc gaggccacgc gtaagggagt
     1081 gcccagggcc cccccgaggg ccgccagcat ggcccccagc atgaacacgc cgctgaagac
     1141 gccggggacg ttcacgccga tggcattcgc catctcccgg tccgaggcgg tggcccgcac
     1201 gatcttgccc caccaggtcc gctcgaagac gagccagagg atgagcgcca cggcgacgcc
     1261 gaaggcaatg atgaacaggt tgtacacggg aaacggccga cccaggatgg accaggcccc
     1321 ttccaggaat cccggaatcg gcgggatccg gaacacgccg ccccagatca tcttcaccgc
     1381 gtcgtccagg atgaggacga agccgaacgt gaggaggagt tgataggcct catccacacc
     1441 gtacaggtat cggaggaaga accgctccat gaccaggccc acgatggcgg tggccaggag
     1501 gctcaaggcg atcgccagga agaagttcca tcccaggagg ccgtagaagg tgaaggcgaa
     1561 gtaggccccg agcatgaaga ggctgccgtg cgcgaaattg atcacgccca ggacgccgaa
     1621 gatgatggtc aggccggccg agatgagcca caggtacatg gccaggctca gtccgatcag
     1681 cccctggaac gccagcgttt gcatgccgga tggctctcct ccaaggacca gaccgcgtta
     1741 gtattgggcc aagagccaat tgaaaacgga caacggacga ctgacaaccg atcaagttcc
     1801 gactttgacc ggttgtcagt tgtcagtttg caaatttgca tttcggcatc gagtcacttg
     1861 ctcgcagaga tgggcgtgaa gggcggccag cggtagtaat ccttgaccgg cagcaccttc
     1921 aaatcaccga ggactgggat gggatacttg ggatccatca tgactcggcc cgccggcaca
     1981 ttgtacacga actgatggtc ctcgcttcgg aagacccgga gacccgccgg cgtgttgatc
     2041 tgcatcccat tgagggcgcc aatcatcttg cccgagtcga ggctgcgggc cttctcgatg
     2101 gctgccttgg cgatcatgac ggccgagtag gtcgtctcgg cggaatagtt ggggaagcgg
     2161 ctccaccgct tc
//
LOCUS       DBNGZC010000151         2707 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1087940, whole genome shotgun sequence.
ACCESSION   DBNGZC010000151 DBNGZC010000000
VERSION     DBNGZC010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1087940"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            521..>2707
                     /locus_tag="AC16G0_02905"
     CDS             521..>2707
                     /locus_tag="AC16G0_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098601.1"
                     /translation="MKPRFGMTARPFLVALLVLAQLWGASGIMAATIPQPPNASPFNI
                     IGFIQKATLDAPGDVLAGGTVTVNGTTVVIPRNTIVQMPNTFLSWAELFTMAPAPWGP
                     GSGPGGVAQTGLALSDVDPGTGKPPLTTYEISLFGNRIVDPADGADKYVAGLVTINQE
                     ALNFGQGFVNFIDYAKGELHIGGIPKVASPTDTRVQINDPVGRFGLKHSPDPRFTADT
                     DNATVHAKTGYPMCIPRTDPAVADDPLGPKVNRPLDAAGNPIGGFTMPPQPAPPATLP
                     AGNADSTKQAPIMVGDFVEYIGTLQQDAAGQYLSAFQIIPNVGIYTAPGNDPAYIVLE
                     DTVLGVGGNPIPGVPQENNNRIVIVGFVTDTSRPVDAYAVDVDPCTGQETERVISTLG
                     PLIPEIIPLGRVRFAQLISNANPPTRNWRLRYNGTSAQTSANGVMAMQYTLPVNAYIF
                     PENTAFGDPTLLAVPLNFQDFPFLALGSGPWRDPNGPIVGQLKPFPLVEIPGMSPAIP
                     TPPVCSPVAVKAPRVQAGTPQTVQAGTPVTLTATGSDPNAPPLPLTFTFTQTAGPAVT
                     LSASSIAAAGGGASVTFTAPTLAAGAAPLTLVFSVTASNGVATSPPSTTFVMVGQQAV
                     RDGVFITVATYDTGRGTLTVNATSTDTRGTATLFLTLDTGFGPIQMTNAGLGGYTFTQ
                     KGIFPTPFTVTVLSSEGGSATANINIRFRAPQIQRRGGGLGGGGALG"
BASE COUNT          538 a          911 c          799 g          459 t
ORIGIN      
        1 acttcaagga caggacacta gcttcgagac acactgaagt agggcttgtg aagcacctcg
       61 gatgtccggt ggggcggtct ctacaagcgg gaaaagctcg caagggaact ttctcctggg
      121 atcggtagtt atgatgtccg aacggtgatg cgtatggagg atgtggtaag tcgtaatgag
      181 ttgaacccac aggcagaaat gaaggatgcc agaaccagaa ctggtgaagg caagaatagc
      241 gattctgagg aggtaacggg tctaggtaat cggaataagt gagagtgaac aaacatcaat
      301 agagccagat agttagccca cgctggatgt atccgcgcga ttcctccacg gaacgcctgt
      361 cctacacgta tctgtcaaag tcctacagaa ccgccagaat tttcccgtca gaaatatcag
      421 ccatcggctg attttcttcc gcacgccctc gccctatcct cttattgcaa gctcagcaac
      481 gctcactctc ccctcgagaa attcttaagg agtatcgtcg atgaagccac gattcgggat
      541 gacggcccgg ccatttctgg tcgcgctcct tgtcctggcc cagctctggg gcgccagcgg
      601 gatcatggct gcaacgatcc cccagccgcc gaacgcctcg ccattcaaca tcatcggctt
      661 catccaaaaa gccaccctgg acgcaccggg cgatgtcctg gccgggggca cggtcacggt
      721 gaatgggacc accgtagtca tcccacggaa caccatcgtt cagatgccga acacttttct
      781 atcatgggca gagctgttca ccatggcgcc ggcgccctgg ggtcccggca gcggtcctgg
      841 aggggtggcc cagaccggtc ttgcgctttc ggacgtcgat cccggcaccg gcaagccgcc
      901 gctcaccacc tacgagatca gtctgttcgg gaatcgcatc gtggatccag ccgacggcgc
      961 ggacaagtac gtcgccgggc tggtgaccat caaccaggaa gcactcaact tcgggcaggg
     1021 cttcgtcaac ttcatcgact acgccaaggg cgagcttcac atcgggggca tccccaaggt
     1081 ggcgtcgccc accgacaccc gggtacagat caacgacccg gtcggccgct tcggtctgaa
     1141 gcactcgccg gacccgcgat tcaccgcgga cacggataac gccacggtcc acgccaagac
     1201 cggttacccc atgtgcatcc cgcggacaga tccggcggtg gccgacgacc cgctgggccc
     1261 gaaggtgaac cgccctctcg acgcggccgg aaaccccatc ggcggcttca ccatgccgcc
     1321 ccagccggcg ccgcctgcca cgctgccggc cggaaacgcc gactcgacga agcaggcccc
     1381 catcatggtg ggggacttcg tcgagtacat cggcaccctc cagcaggatg cggccggcca
     1441 gtacctgtcg gccttccaga tcattcccaa cgtggggatc tacaccgccc cggggaacga
     1501 tccggcgtac atcgtgctgg aagacacggt gctcggcgtg ggcggcaacc cgatccccgg
     1561 cgtgccgcaa gagaacaaca accgcatcgt gatcgtcggc ttcgtcacgg acacctctcg
     1621 ccccgtggat gcgtatgccg tggatgtgga cccctgcacg ggccaggaga cggagcgggt
     1681 catcagcacg ctcggcccat tgatcccgga gatcatcccc ctcggtcgtg tgcgctttgc
     1741 acagctcatc tcgaacgcca accctcccac tcgcaactgg cgactccgct acaatgggac
     1801 cagcgcccag acctccgcca acggcgtcat ggccatgcag tacaccctgc cggtgaacgc
     1861 gtacatcttc ccggagaata ccgcgttcgg cgatccgacc ctgctcgccg tcccactcaa
     1921 cttccaagac ttccccttct tggcgctggg atccggcccc tggcgtgatc cgaacgggcc
     1981 cattgttggg caattgaaac ccttcccgct ggtggagatc cctggcatga gcccggcgat
     2041 ccccacgccg ccggtgtgca gtccggtggc ggtcaaggct ccacgggtcc aggccggcac
     2101 acctcagacg gtccaggccg gcacacccgt gaccctgacg gcgacaggaa gtgatcccaa
     2161 cgcgcccccg ctgcccctga ctttcacctt cacgcagacg gcggggccgg ccgtgacgct
     2221 ttccgccagc tccatcgcgg cggcgggcgg cggggcaagc gtcacgttca cggcaccgac
     2281 gctggctgcc ggggctgccc cgctcacgct tgtcttcagc gtcacggcga gcaacggggt
     2341 cgccaccagc ccgccgtcca cgaccttcgt gatggtcggg caacaagcag tgagggatgg
     2401 ggtgttcatc acggtagcta cctacgacac aggacgaggg accctcaccg tgaatgcaac
     2461 gtccacggat accaggggga ctgccacgct cttcttgacg ctcgacacgg gcttcggccc
     2521 gattcagatg acgaacgcag gtctgggggg ctacaccttc acgcaaaagg gtatcttccc
     2581 cacgccattc accgtcaccg tgctctcgag tgagggaggc agcgccacgg caaacatcaa
     2641 catcaggttc cgtgcaccgc aaatccagcg gaggggcggc ggcttgggcg gcggcggtgc
     2701 cttgggc
//
LOCUS       DBNGZC010000152         1513 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_12780, whole genome shotgun sequence.
ACCESSION   DBNGZC010000152 DBNGZC010000000
VERSION     DBNGZC010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1513)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_12780"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="AC16G0_02910"
     CDS             complement(<1..302)
                     /locus_tag="AC16G0_02910"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HIR5098602.1"
                     /translation="MLEPDGTELTAAEKQRYARQMIIPNWGEETQRKLKASKIFIAGA
                     GGLGSPVSFNLAVAGIGLIRICDFDSPELSNLNRQFLHDDSRVGMNKAESAKRTLS"
     gene            complement(596..>1513)
                     /locus_tag="AC16G0_02915"
     CDS             complement(596..>1513)
                     /locus_tag="AC16G0_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR5098603.1"
                     /translation="PLAHWVWGTGGWLHNLGALDFAGGTVVHISSGISALAAALLIGR
                     RHGFGSEPMPPHNLPLTVTGAAMLWVGWFGFNAGSALSSGALATSAFVVTHVATAAAT
                     ISWMLTEWLSRGKPTVLGAASGAVAGLVAITPASGFVGPLSAVVIGAVGGYLCYNACN
                     FKTRLGYDDSLDVVGVHGVGGTWGALATGLFASKVINDAGNNGLFFGNPGQLWIQVVG
                     ILATWVFAFIGSLILLKIVDLVIGLRVYEEDEILGLDLSQHNENAYTFGLVGTGGIGT
                     GFGTSEYRAVQGHTTLGGTSSVSPRAAMD"
BASE COUNT          277 a          482 c          501 g          253 t
ORIGIN      
        1 ctggacaggg tccgcttggc cgactcggcc ttgttcatcc cgacgcgcga gtcgtcgtgg
       61 aggaactgcc gattgagatt ggagagttcc ggggaatcga agtcgcagat ccggatgagg
      121 ccgatacccg cgaccgccag gttgaaggac accgggcttc ccaggccgcc ggcccctgcg
      181 atgaagatct ttgacgcctt gagctttcgc tgggtctctt ccccccagtt ggggatgatc
      241 atctgccgag cataccgctg cttctccgcg gccgtgagtt cagtcccgtc gggctcgagc
      301 atcacctcgt acctcctcgc tggcaccatg tacggtattg acgggtgggg aagatcccgc
      361 tgcggcttgc gcgcattcac cctcccggta cgtgtagccc aagggggagc caaagtcaat
      421 gcaacggttg gtcaatcttc ggcgggtgac acggatgggg caaacgcatt ggcactcggg
      481 gcgcgtgacc ggacgccctc cgcggaagcg cacgaagcgg gccgggcacc ctggagtgct
      541 cgagggtgca gacaagggct tgggtcccgt caccgctcag gcgatgtggt ccggatcagt
      601 ccatggccgc cctgggagag acggatgagg tccctccaag ggtcgtgtga ccctggactg
      661 cccggtattc cgaggttccg aagcctgtgc ctatcccgcc ggtcccgacc agcccgaagg
      721 tgtaggcatt ctcgttgtgc tggctgagat ccaggcccag gatctcgtcc tcttcataga
      781 cgcgcaggcc gatcacgagg tccacgatct tgaggagaat gagcgagccg atgaaggcaa
      841 agacccaggt ggccaggata ccgacgacct ggatccagag ctgaccggga ttgccgaaga
      901 agagcccgtt gttgcccgca tcgttgatca ccttgctggc gaagaggcct gtcgccaggg
      961 cgccccacgt ccctccgact ccatggacgc cgaccacatc cagggaatca tcgtagccga
     1021 ggcgggtctt gaagttacag gcgttgtaac acagataccc tccgacggcc ccgatgacca
     1081 cggccgacag aggccccacg aaaccagagg cgggtgtgat ggccacgaga cccgcgacgg
     1141 cgccggaggc agccccgagg acggttggtt tcccccggct cagccattcg gtgagcatcc
     1201 aggagatcgt tgctgccgcc gtcgccacgt gggtgaccac aaaggcgctg gtggccaggg
     1261 cgccggaact caaggcgctc cctgcattga agccgaacca gccgacccag agcatggccg
     1321 cgcccgtgac ggtcaggggc aggttgtggg ggggcatggg ctcggagccg aagccatgtc
     1381 gtcttccgat caggagcgca gccgccaggg ccgagattcc ggagctgatg tggaccacgg
     1441 tcccccccgc gaaatccagg gccccgaggt tatgcagcca gccgcctgtt ccccataccc
     1501 agtgagccag cgg
//
LOCUS       DBNGZC010000153         2273 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_207967, whole genome shotgun sequence.
ACCESSION   DBNGZC010000153 DBNGZC010000000
VERSION     DBNGZC010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_207967"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            501..1322
                     /locus_tag="AC16G0_02920"
     CDS             501..1322
                     /locus_tag="AC16G0_02920"
                     /inference="COORDINATES: protein motif:HMM:NF015056.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HIR5098604.1"
                     /translation="MATIDAGAEICFETWDARTGTVQTERDLLATPHPRGPNPATGPV
                     AIRGALPGDTLSVEILDITLGSRGYTGIRPGRGILGHLIGDYRTKVFQIQDGMVHFND
                     RIRFPVRPMVGVIGTTPAGGAIGNLHPGPHGGNMDHNDVRVGAKVHLPVFIPGALLAI
                     GDVHACMGDGEISITALEICGEVRVRVDLAKDAGIARPWIEFPDCWITTGDGPSVGDA
                     IRVACEEMAALLQRGLGLSVEDAYMLLSIRGDVKVSQCCDPATIAATARVVMPKM"
     gene            1446..1877
                     /locus_tag="AC16G0_02925"
     CDS             1446..1877
                     /locus_tag="AC16G0_02925"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR5098605.1"
                     /translation="MSKVLAVTALELQEILSGTRFTRGYGFRFHAIGDGECTLHVPFQ
                     ESFERPGGVVSGQVYMAAADVAMWLAIKTKRGKDDPSVTVAMQTTFMRSARREDLRCT
                     ARLLKMGRRLIHGVAECVTLEGKLLSHHTITYMQPGTAGSD"
     gene            2096..>2273
                     /locus_tag="AC16G0_02930"
     CDS             2096..>2273
                     /locus_tag="AC16G0_02930"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR5098606.1"
                     /translation="MSLRSKVYAAFLEMERAAYCDGALPKKAKELIAVGISVVMDCES
                     CMQWHIEQAAKAGAS"
BASE COUNT          449 a          665 c          771 g          388 t
ORIGIN      
        1 tagatcccgc gccgcacctt tctctcgcct ctgcactccc atcgcgataa tctccaccat
       61 cccctcgcca agcggcgtcg ctctcatccg tccccgcata gccgcgaacc aagcctcagc
      121 cgggcgcccc ttggcccctt cgtagaagat ccatatttct cgtttgccac caacaagaca
      181 cgttccgagg cgaaacttag cggcacttca gtgtcaattt tgggcaccat cctccattgc
      241 ggagtttgaa ggtggtgact gcacgagaac tcggaggctc cgtcatttcc cttgggcgtt
      301 tgggcatgct ataaaatgag actgcgacag gaggggcgag cggcgcggag gatctgcacg
      361 cagaagcgat ggagctatgc tgaagtgccg ctggaatgag cggccagagc gggaatcctg
      421 cgacgactgc gaggggtgag aagatgatcc agatcggccg agagcatatc gtgtattctt
      481 ttgatagggc gcatgctccc gtggcgacaa ttgacgcggg ggccgagatc tgcttcgaga
      541 catgggacgc ccgcacaggg acggtgcaga ctgagcgtga cctcctggcc acgccgcacc
      601 ccagggggcc aaacccggcc accggtcccg tggcaatacg cggcgccctg cccggcgata
      661 ccttgagtgt ggagatcctc gacatcaccc tgggatctcg ggggtacacc gggattcgac
      721 ccggacgagg tatcctggga cacctgatcg gagactatcg gaccaaggtc tttcagattc
      781 aagatgggat ggtccacttc aacgaccgga tccgcttccc ggtgcggccc atggtggggg
      841 tgatcgggac cacgcctgcc ggcggggcca tcgggaacct ccaccccggg ccgcacggcg
      901 gcaacatgga tcacaacgat gtccgcgtcg gcgcaaaagt tcacctgccg gtcttcatcc
      961 ccggcgcgct cctcgcgatc ggagacgttc acgcctgcat gggtgacggc gagatttcca
     1021 tcaccgccct ggagatctgt ggtgaagtca gggtgcgcgt cgatctcgcc aaggatgcag
     1081 ggatcgcgcg accgtggatc gagttccccg actgctggat cacgaccgga gatggaccct
     1141 cggtggggga tgcgatccgg gtggcgtgcg aggagatggc cgcactcctc cagaggggcc
     1201 tcggtctgtc ggtagaagac gcgtacatgc tgctgagcat ccggggtgac gtgaaggtgt
     1261 ctcagtgctg cgaccctgca accattgcag ccacggctcg ggtggtcatg cccaagatgt
     1321 aactgggtgg cgcgccggtg attggcgacg cgcctgcaac ctgggcaggc gggcggatcc
     1381 atggggggag ccgcacggga gttcaatgga tggatcgacc ggattcccga gaggaggggg
     1441 ggacgatgag caaagtcctg gccgtgacag cactggagct tcaagaaatc ctatcgggga
     1501 cgaggttcac acggggatac ggattccggt tccatgcgat cggggacggg gaatgcacgc
     1561 tccacgttcc attccaggaa tcctttgaac ggcctggagg ggtggtgagc ggccaagtct
     1621 acatggccgc agcggacgtc gccatgtggc tggccatcaa gacgaaacgc ggcaaggacg
     1681 atccctccgt cacagtcgcc atgcagacga cgttcatgcg cagtgcgcgg cgggaggatc
     1741 tccggtgcac cgcgaggctc ctgaagatgg ggaggcgact gatccacggg gtggcagagt
     1801 gtgtcacact cgagggcaag ctcttgagcc atcacacgat cacctacatg cagcctggta
     1861 cggcaggatc cgactaggcg gacgaggggc gaccgtctgg caacgcagga agacaaggga
     1921 tgcgacgctg ctctcccggt gcggcgcggg gcgtggcgtg ccggctccgg acgcccggac
     1981 cgaagcgaac gggacacagg gcgccgcctg gagagcgggg cagacgtccc gaggagactc
     2041 catggataga ctcgaggaga tttcacgcag taggaaggcg gcgcacacct agctcttgtc
     2101 gctcaggtca aaggtctacg cggcgttcct ggagatggag agggcggcct actgcgacgg
     2161 tgcgctgccg aagaaggcaa aagaattgat cgcggtgggt atttccgtgg tcatggactg
     2221 cgaatcgtgc atgcagtggc acatcgagca agcggcgaaa gccggagcct cct
//
LOCUS       DBNGZC010000154         2239 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_892709, whole genome shotgun sequence.
ACCESSION   DBNGZC010000154 DBNGZC010000000
VERSION     DBNGZC010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_892709"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="AC16G0_02935"
     CDS             complement(<1..593)
                     /locus_tag="AC16G0_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR5098607.1"
                     /translation="MEIIQQGTADIRFTHPDDFREWVRLHKRREMVEKLMTEREAISR
                     YVVDGDYLAYDFSSLTRGPQALVREIIRQRRKDLWICAEFTLHESSLLVGSGCASRID
                     VGFLGYGNYIGRAVSEGRVRVFDWTNGALASRMLAGARGIPFMPMRSLLGTDSLRRSG
                     AKVVHDPFTGSPICLVPALNPDVTLLHVHQADAYGNAR"
     gene            complement(713..1651)
                     /gene="meaB"
                     /locus_tag="AC16G0_02940"
     CDS             complement(713..1651)
                     /gene="meaB"
                     /locus_tag="AC16G0_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HIR5098608.1"
                     /translation="MDLVEGILRGDVRSAARLMTLVENGAAGSVAALKALYPFTGRAH
                     VIGVTGPPGSGKSTLTDRITEELRRREKTVGIVAVDPTSPFTGGAILADRIRMQRHSL
                     DDGVFIRSMATRGHLGGLARATNDVVDVMDAMGKEYVLIETVGVGQDEVEVVKAAHTS
                     IVVAVPGLGDDIQAIKAGIMEIGDLFVVNKADLGTADKTVMEIEMMLHLGESEEGWVP
                     RIYKTVASRNEGVVELTQAIQEHQAFLEKEGGRREKSRERSQWVFWQLLQEQMTSRVI
                     DRVANNGTLDRLIKDIAMRETDPYSAVEDVLRRVGL"
     gene            complement(1853..>2239)
                     /locus_tag="AC16G0_02945"
     CDS             complement(1853..>2239)
                     /locus_tag="AC16G0_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR5098609.1"
                     /translation="VAHLTRFGVGVTSVGLVEAAAFLFIGCFIDAVPAIIIIAPILAP
                     LAAKVGMHPIHFAIIGVVSLAFGLVTPPYGLCLLIDVAIARTSVSHVIKDVAIILLPM
                     LVMLFLIILFPQVVLLIPRLIMPKFV"
BASE COUNT          438 a          752 c          664 g          385 t
ORIGIN      
        1 cgggcgttcc cgtacgcgtc ggcctgatgg acgtgaagca aggtgacatc cggattcaga
       61 gcgggcacca ggcagatcgg acttccggta aacgggtcgt ggaccacctt ggcacccgag
      121 cggcgcaggc tgtcggtccc cagcaggctc cgcattggca tgaaggggat cccgcgcgcg
      181 cctgcgagca tccgagacgc cagggccccg ttggtccagt caaagaccct cacccggcct
      241 tcgctgaccg cccggccgat gtaattgccg tagccaagga accccacgtc gatccggctg
      301 gcgcagccgc tgccgaccag gagtgaactt tcgtgcaggg tgaactcggc gcagatccag
      361 agatccttcc gccgctggcg gatgatctct cgaaccaggg cttgagggcc gcgtgtcagg
      421 gaggaaaaat cgtaggcgag gtaatcgccg tcgacaacat agcgggagat ggcctcccgc
      481 tccgtcatga gcttctccac catctcccga cgtttgtgca ggcgcaccca ctcgcgaaaa
      541 tcgtcgggat gggtgaagcg gatgtctgcc gtaccctgct ggatgatctc catgcccccc
      601 tcacaggtcc aggagaaccg acggcagcgg gaaagacgga actggtcagc ggaccaaggt
      661 cttccgatgt cgtcctttcc cgaagaagac ccagcccggg ctccggtgaa gactacaatc
      721 cgacccgccg cagcacgtcc tccaccgcag aatacggatc ggtctctcgc atcgcaatgt
      781 ccttgatcag ccggtccaaa gttccgttgt ttgcgacccg atcgatcacg cgggacgtca
      841 tctgctcctg gaggagctgc cagaagaccc actggcttcg ttcgcggctc ttctcccgcc
      901 gccccccctc cttctccaga aaggcctggt gctcctggat ggcttgcgtc aattccacga
      961 ccccttcgtt acgcgaggcg acggtcttgt agatcctcgg cacccatccc tcctcggact
     1021 ccccgaggtg gagcatcatt tcaatctcca tgaccgtctt gtcggcagtc cccaggtcgg
     1081 ccttgttgac cacgaagagg tccccgatct ccatgatgcc cgccttgatg gcctggatat
     1141 cgtcgcccag tcccggcacc gccaccacga tactcgtgtg ggcggccttc accacctcga
     1201 cctcgtcctg gcccaccccc acggtctcga tgagaacgta ctccttgccc atggcgtcca
     1261 tgacgtccac cacgtcattg gtcgcccgcg ccaagccgcc gagatgcccc cgggtggcca
     1321 tgctgcggat gaagaccccg tcatccaggc tgtgccgctg catccggatc cggtcggcca
     1381 ggatggcccc gcccgtgaac gggctcgtcg ggtccacggc cacgatgccg accgtctttt
     1441 ctcgtcggcg cagctcctcc gtgatccgat ccgtgagcgt gctcttgccc gagcccggag
     1501 ggccggtcac tccgatgaca tgcgccctgc ccgtgaatgg atagagggct ttgagcgccg
     1561 ccacggaacc ggccgccccg ttctcgacca gggtcatgag gcgggccgcc gagcgcacgt
     1621 cgccccgcag aatcccttcc acgagatcca taacagtgag aaccttcagt tccaaaacgg
     1681 tgtttgtggt tcgggaacct accacagcaa tcccgggcgg tccactaaaa tcaagggaat
     1741 agaggtaggc acaccgggcc ggaaggcatt tcgacgaccc aagaaaaacc aaaccggaga
     1801 gcgccgcatc ctacgccctc cggttccgga tggtgcctgc cgccggctga gtttaaacga
     1861 acttgggcat gatcaggcgc ggaatcaaca acactacttg tgggaagagg atgatgagga
     1921 agagcatcac cagcatcggc agcaggatga tggccacatc cttgatcacg tgtgacaccg
     1981 acgtccgggc gatggccacg tcgatcagca ggcacaatcc gtacggcgga gtcaccaggc
     2041 cgaatgccag cgacacgaca ccgatgatgg caaagtggat ggggtgcatc ccgaccttcg
     2101 cggccaaggg tgccaggatc ggcgcgatga tgatgatggc cggcacggcg tcgatgaagc
     2161 agccgatgaa gagaaaggca gccgcctcca ccaggccgac actcgtcact ccgacgccga
     2221 accgcgtgag gtgagctac
//
LOCUS       DBNGZC010000155         1810 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_208011, whole genome shotgun sequence.
ACCESSION   DBNGZC010000155 DBNGZC010000000
VERSION     DBNGZC010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_208011"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="AC16G0_02950"
     CDS             complement(<1..549)
                     /locus_tag="AC16G0_02950"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5098610.1"
                     /translation="MPLKGFPKQIALRDHTAITLRPMVREDGPALLGFFRRLSPEDRQ
                     FLKDDVTRPDIIEAWVRGLDYDRVLPILAEIEGRIVGDATLHRNAYGWMRHVGEIRVV
                     TDAYFRRRGLASSMAREIFYLALQAGLDKMVAEMGVDQVAAIKVFQKLGFQQEARLAN
                     HIIDLHGRKHDLILMTTDIPALM"
     gene            complement(712..1482)
                     /locus_tag="AC16G0_02955"
     CDS             complement(712..1482)
                     /locus_tag="AC16G0_02955"
                     /inference="COORDINATES: protein motif:HMM:NF014017.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HIR5098611.1"
                     /translation="MIQIGTSGFSYPEWRGVFYPEKLPQQEMLAFYARHFRAVEINST
                     YYHIPPARNMAAMAKRAEGRLAFAVKAHQDMTHDRDKYAGALPAFREALAPLQEAGAL
                     GCVLVQFPFSFKAIPANVDFLRRVAGDLVPHPLVVEFRHRSWTTDETFSLLRDLRVAY
                     CCVDEPRLPNLPPPVTQATSPLAYVRFHGRNRQKWWTHTQAWERYDYLYTEAELQEWV
                     PHLRALADATEKCYAFFNNHARGQAVTNAQMLARLLAS"
BASE COUNT          344 a          531 c          605 g          330 t
ORIGIN      
        1 catgagcgcg gggatatcgg tggtcatcag gatgaggtcg tgcttccgcc cgtggagatc
       61 aatgatgtga ttggccagtc gtgcctcttg ttggaagccg agcttctgga acaccttgat
      121 ggcagcgacc tgatccacac ccatctcggc caccattttg tccaggcccg cctgcagggc
      181 gaggtaaaat atctcccgag ccatgctgga agccagcccg cgtctccgga agtacgcatc
      241 cgtgaccaca cggatttccc ccacatggcg catccacccg taggcgttgc ggtgcagcgt
      301 ggcgtccccc acgatccgcc cttcgatctc ggccaggatg ggcaggaccc gatcgtagtc
      361 cagcccccga acccacgcct cgatgatgtc cgggcgtgtg acgtcgtcct tcaagaactg
      421 ccggtcttcc ggcgagagcc ggcggaagaa cccgagaagt gccggcccat cttcccggac
      481 catggggcgc agggtgatgg cggtatgatc gcggagggcg atctgctttg ggaatccctt
      541 gagaggcatg gcgcgcctcc tcgaagcggt gaggttcgtt cagcttgggg gatgatgtcc
      601 actgcaagct agcagaggtc ccgtcagcga gcaagggggg agaggagtcc ccgggtggaa
      661 tgtcaggaaa tccagcccac tcgtgaaata gtgtaggcgg gtggggggcc gtcacgaagc
      721 gagaagacgc gcgagcatct gggcgttggt cacggcctgg ccccgcgcgt gattattgaa
      781 gaaggcgtaa cacttctccg tggcatcggc cagggcgcga agatggggga cccattcctg
      841 gagctccgcc tcggtgtaaa ggtaatcgta gcgttcccag gcctgggtgt gcgtccacca
      901 cttttggcgg tttcgcccgt ggaatcgaac gtaggccaac ggggaagtgg cctgcgttac
      961 gggcggcggc aaattgggga gccgcggctc gtccacgcag cagtaagcca ccctgagatc
     1021 cctcaatagg ctaaaggtct catcggtggt ccagctccgg tggcggaact ccaccacgag
     1081 aggatgggga acgaggtctc cggcaacgcg gcggaggaag tcgacgttgg cgggtatggc
     1141 cttgaatgaa aagggaaact ggaccagcac gcagcccagg gcgccggctt cctgcagcgg
     1201 ggcgagggcc tcccggaagg ccgggagggc gcccgcgtac ttgtcgcgat catgggtcat
     1261 gtcctgatga gccttgaccg cgaatgccaa tctgccctcg gcccgcttgg ccatggctgc
     1321 catgttgcgg gcaggcggaa tgtggtagta ggtggaattg atctcgaccg ccctgaagtg
     1381 tcgagcgtag aacgccagca tctcctgctg ggggagcttc tccgggtaga agactccccg
     1441 ccactccggg taactgaagc cgctggtgcc gatctggatc atgcgaggcc cggctccgat
     1501 cgtctgccaa agtcctcatt cctccgatcg atctcgaaga aatcccgtag ccggcgcatt
     1561 ctggaccagc aggtcgggct gaatcggaat tgcatcgccg gcagggccgc atggaaaacg
     1621 gcatcgctcc aaagcacgat acgggttctg gcgcatggcg ggatggcgac gtcgccctcg
     1681 gccagataga gtcggagtga ccctctgtgc tcagcacgcc agtctgtgga gacatgcaga
     1741 ctgccagaga cgataccagg aacagatgct cgcagcgaag ggtgcggttc ccttcgtatc
     1801 cctgtctcgt
//
LOCUS       DBNGZC010000156         1847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2164068, whole genome shotgun sequence.
ACCESSION   DBNGZC010000156 DBNGZC010000000
VERSION     DBNGZC010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2164068"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..643)
                     /locus_tag="AC16G0_02960"
     CDS             complement(50..643)
                     /locus_tag="AC16G0_02960"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR5098612.1"
                     /translation="MSFIDYAAREINCKLVYYGPGLGGKTTNLRHIYARVDPGARGRL
                     ISLATETERTLSFDFLPLELGSIHGFKTRFRLYTVPGQVFYDASRKLILRRTDGVVFV
                     ADSQIERLESNIESLENLEANLAEQGDILGRLPFVVQYNKRDLPNILSVEALQGALNA
                     RSAPWFEAVATQGVGVFETLKGAAKLVLGELKKTTLA"
     gene            complement(748..1350)
                     /gene="recR"
                     /locus_tag="AC16G0_02965"
     CDS             complement(748..1350)
                     /gene="recR"
                     /locus_tag="AC16G0_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR5098613.1"
                     /translation="MSTTPPALATLIEQLVRLPGIGPKTAQRLAFHLLKVPREHAAGL
                     ADAILALKDRTRLCTRCFNIAETEICGVCQDPRRDPKLLCVVEEVNDLLAIEKTREFR
                     GLYHVLGGSLSPLDGRGPDQIRGKELLVRLEAGEIREVILATNPNVEGEATALYLLRL
                     LKSFPVRVTRIARGLPMGGDLEYADEATLARALEGRREMG"
     gene            complement(1369..1683)
                     /locus_tag="AC16G0_02970"
     CDS             complement(1369..1683)
                     /locus_tag="AC16G0_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR5098614.1"
                     /translation="MPGLGDIMKQAQKMKAEMDRIQAEVAAKTVEGSAGGGMVNVLAN
                     GKGELLSVKIDPEVIRNEDLEMLQDLVTAAANEALRKARELLAQEVSRLAGGLGLPPG
                     LM"
BASE COUNT          300 a          621 c          577 g          349 t
ORIGIN      
        1 gtaactatac cagcagtacg tattcgccgc aagtcgctcg cgggcgggct cacgccagcg
       61 tcgtcttctt cagctcgccc aagaccagct tggccgcgcc cttcaaggtc tcgaagacgc
      121 cgaccccttg ggtggcaacg gcttcaaacc agggggcgct gcgcgcgttc agtgccccct
      181 ggagcgcctc gaccgagagg atatttggca agtcccgctt gttgtactga acgacgaagg
      241 gcaggcgacc caggatgtct ccctgttccg ctagattcgc ctccaagttc tccaggctct
      301 cgatattcga ctcgagtctc tcgatctggg aatcggccac gaagacgacg ccgtccgtcc
      361 gacggaggat cagcttccgg ctggcgtcat agaaaacctg gcccggcacc gtgtagaggc
      421 ggaagcgggt cttgaagcca tggatgcttc ccagttccag ggggaggaaa tcgaaggaga
      481 gtgtgcgctc tgtctccgta gccagggaga tgagcctccc cctggcgccg ggatccacgc
      541 gggcgtagat gtgtcggagg ttcgtggtct ttccgcccag cccaggcccg taatagacca
      601 gcttgcagtt gatctcgcgc gccgcataat cgatgaagga cacgccgtcg tcctagcctg
      661 cggggggctg ccgatatccc tctcggagat ctgggcgagc tccagctaac gctctgcttc
      721 cacaccgggc gcttctgaat ccggtcctca gcccatctcg cgccgtccct ccagggcgcg
      781 agccagcgtt gcttcgtcgg cgtactccag gtccccgccc atgggcagac cgcgggcgat
      841 gcgcgtcact ctcaccggga atgacttgag gagccgcagt aggtagagcg ccgtggcctc
      901 tccctcgacg ttggggttcg tggccaggat gacctcgcgg atctcgccgg cctccagacg
      961 caccaggagc tccttccccc ggatctggtc cggtccccga ccatccaggg gggaaagaga
     1021 cccgccgagg acgtgataca gcccgcggaa ctcccgcgtc ttctcgatcg ccagcaggtc
     1081 gttgacctcc tccaccacgc acaggagctt cggatcgcgg cgcgggtcct ggcagacccc
     1141 gcagatctcc gtttcggcga tgttgaaaca gcgggtgcac aggcgcgtcc ggtccttcag
     1201 cgccaggatc gcatccgcca gccccgcggc gtgctctcgc gggactttta gcaaatgaaa
     1261 ggccagccgc tgggccgtct tgggaccgat ccctgggagg cggaccagct gttcgatgag
     1321 cgtggcgagg gcagggggag ttgtggacat gccggcttct cctcctgcct acatcagacc
     1381 cggcggcaga ccaaggccgc cggcgagtcg cgagacttcc tgcgccaaga gctcacgtgc
     1441 tttgcgcaag gcctcattgg ccgccgccgt caccagatct tgcagcatct ccagatcctc
     1501 gtttcgaatg acctcggggt cgatcttcac ggagagcagc tcccctttcc cgttggccag
     1561 cacgttgacc atccccccgc cggcgctgcc ctcgacggtc ttggcggcca cctcggcctg
     1621 gatgcggtcc atctcggcct tcatcttctg ggcctgtttc atgatgtcgc cgagtcccgg
     1681 catgcgtcct cctcagatgc gagcctcgtg aatgatcgtc ccgccgaaca ggttgagtgc
     1741 ttcgcgtacg agcggatgct cccggacgtc cggtgaggca gagcgggcag gagtctcggc
     1801 cggctctggc tgtggcgttg cgcttgccac aaaccggtac tcgaggc
//
LOCUS       DBNGZC010000157         3250 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1871579, whole genome shotgun sequence.
ACCESSION   DBNGZC010000157 DBNGZC010000000
VERSION     DBNGZC010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1871579"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1329
                     /locus_tag="AC16G0_02975"
     CDS             <1..1329
                     /locus_tag="AC16G0_02975"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005269493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HIR5098615.1"
                     /translation="CDNLYLNYPAGGNYREMQSFLWFHDHRMHHTGANVYKGMVGLMP
                     HYAPDDNGDETNPNGLGLPGRRVNNPDGSFDVKYDIPFALYDVALDDGVTPHADFHNG
                     CGELHPEWWGKQYFRHFPNHGFVGDIFTVNGKAYPVLHVYQRKYRLRFLDCSISRIYQ
                     LWLMRSGSGPTPKPGMQGQWGLPDGQQCMRFTQIASEGGLLPRPIVRNSFEMWPAKRR
                     EFVVDFTKYMDGSPTRPGDVIYLVNSLSMPTGRIPDNFPPDPNQVKDSRTLVPMVKIV
                     IDGPPPEPDYSVIPSVLRPMPDLPANLSSLPQRRFVLEHGSPANPLGPTSGETEWLIN
                     GLQFDPLNPLALPKQWQPEIWTVQNGGGGWVHPMHMHMEEHHVLSRNGVPAPDSLHPD
                     DTGREDVAALYPSEAVTFYRNFRTFKGKYVAHCHNLAHEDHNMMFGWAIV"
     gene            1417..1848
                     /locus_tag="AC16G0_02980"
     CDS             1417..1848
                     /locus_tag="AC16G0_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098616.1"
                     /translation="MTEKGSAPIMHEILVIKRTTAALGGLRQRLGFGYPRLALGARRH
                     SNVQIPAFLAAALAAVLTLAAPDLAPANVESARESQESQAQHENPAAGDHAPQPQQEG
                     AVEEIQEFQPQNDGAAAEADAPQPQPSIKATPQWPKWQPWQ"
     gene            1928..>3250
                     /locus_tag="AC16G0_02985"
     CDS             1928..>3250
                     /locus_tag="AC16G0_02985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5098617.1"
                     /translation="MKRTTYAILPFFLIAGVFLAGAWYGQQGAVTAVTAAPSSARQIL
                     YYVDPMHPAYKSDKPGIAPDCGMELVPVYSDGTMGEAGSSPAEAPGTVTINPERQQLI
                     GVQVSSVAKAAVTRSLRLLGRVAPDESRIYKLNSGVEGFIREVAPVTTGDHVTKDQLL
                     ATFSAPNAYNVIQLYILNLGAVDRIVQSAAAGSVDAQAAPAGAANVQLRVDQMESLGV
                     SPHQMAEIRRTRQIPPSIQILSPADGIVIARNVSPGVKFDKGTEWYRIADLRRVWILV
                     DVFENEAQYLQPGKVVTVSLRQQNKTFQAKVSPVRPQFDPATRTLKVRLEADNLGDIL
                     RPDMFVDVELPVSTPPMISVPVDAVVDSGLTQTVFVDRGEGRFEPRRVETGGHFGDRV
                     EIVKGLMEGERIVVSGIFLLDSESRMKTVAGATSPMHAAHGQHADGAMD"
BASE COUNT          610 a         1109 c         1015 g          516 t
ORIGIN      
        1 tgcgacaacc tctatctcaa ctacccggct ggcggcaatt accgcgagat gcagtccttc
       61 ctctggttcc acgatcatcg catgcaccac accggggcca acgtctacaa gggcatggtg
      121 ggactcatgc cgcactacgc ccccgatgac aatggtgacg agaccaaccc caatgggctc
      181 ggcctgcccg gccgccgcgt gaacaaccct gatgggtcct tcgacgtgaa gtacgacatc
      241 cccttcgccc tgtacgacgt cgccttggac gacggcgtga cgccgcacgc ggacttccac
      301 aatggctgcg gcgagctgca ccccgagtgg tggggaaagc agtatttccg ccacttcccc
      361 aaccacggct ttgtgggcga catcttcacg gtcaacggga aggcctaccc cgtgctccac
      421 gtctaccagc ggaagtaccg gctccgcttc ctggactgct cgatctcgcg gatctaccag
      481 ctctggctca tgcgctcggg cagcggcccc acgcccaagc cgggaatgca gggccagtgg
      541 ggactccccg atggccagca gtgcatgcgc ttcacccaga tcgcgtccga gggcggcctg
      601 ctgccccggc cgatcgtgcg caactccttc gagatgtggc cggcaaagcg gcgcgagttc
      661 gtcgtggact tcaccaagta catggacggc agcccgaccc ggcctggcga cgtgatctat
      721 ctcgtgaaca gcctctcgat gccgaccggg cggattccgg acaacttccc gcccgacccc
      781 aaccaggtca aggactccag gacgctggtg ccgatggtga agatcgtcat cgacgggccg
      841 ccgcccgagc cggattacag cgtcatcccc tcggtgctgc gccccatgcc ggacctcccg
      901 gcgaacctct cgagcctgcc gcagcgcagg ttcgtgctgg agcacggctc cccggccaac
      961 ccgctgggac caaccagcgg cgagaccgag tggctgatca acggcctcca gttcgatcca
     1021 ctcaacccgt tggccctccc caagcagtgg cagcccgaga tctggaccgt ccagaacggc
     1081 ggcggtggct gggtgcatcc gatgcacatg cacatggaag agcatcatgt cctgtcccgc
     1141 aacggggtgc cggcgcctga ctccttgcac ccggatgaca ccggccgaga ggacgtggct
     1201 gcgctctatc ccagcgaggc ggtaacgttc taccggaact tccggacgtt caagggcaag
     1261 tatgtcgccc actgccacaa cctggcccac gaagaccaca acatgatgtt cggctgggcg
     1321 attgtttagt tgattttgga gtgaccccga ccccccggcg cacccgcgcc ggggggtcac
     1381 cagccctgca ggtcgccagg agagtgaccc cctggcgtga cagagaaagg gtcagccccc
     1441 atcatgcacg agatcttggt tatcaaacgg actaccgcgg ctctcggcgg tctccggcag
     1501 agactcggat tcggctaccc gcgtctcgcc ttgggtgccc ggagacatag caacgtgcaa
     1561 atccccgcct tcctggccgc cgccctggcg gccgtcctca cgctcgcggc cccggacctc
     1621 gctccggcga acgtcgagag cgcccgcgag tcccaagaat cccaagcgca gcacgagaac
     1681 ccggccgcag gcgatcatgc gccacagccg cagcaggagg gcgcggtcga agagatccaa
     1741 gaattccagc ctcagaacga tggcgcggcc gcagaggccg atgccccgca accccagccc
     1801 tctatcaaag ccacgccgca gtggccgaag tggcaaccat ggcagtgatg ggctggttcg
     1861 aagggatcat tcgcggagtg ggcgcagctc acgagtattc agaggaaaga ggagaaaggt
     1921 gtcttcaatg aaaagaacaa cctatgccat cttgccattc ttcctgatcg ccggggtgtt
     1981 cctggccggc gcctggtacg gccagcaggg cgccgtcacg gccgtcacgg ccgccccctc
     2041 cagcgcccgt cagatcctct actacgtgga cccgatgcac ccggcctaca agtccgataa
     2101 gcccgggatc gcgccggatt gcggcatgga actcgtgccg gtctattcgg acggaaccat
     2161 gggcgaggcg gggagcagcc cggcggaagc gccgggaacc gtgaccatca accccgagcg
     2221 gcaacaactg atcggggtgc aggtgagctc ggtggcgaaa gcggccgtca cccggtcgct
     2281 ccgcctcctg ggccgcgtcg cccctgatga gagccgaatc tacaagctca actccggggt
     2341 cgaaggcttc atccgcgagg ttgcgccggt gacgaccggc gaccacgtga ccaaggacca
     2401 actcctcgcc accttctcgg cgccgaacgc ctacaacgta atccaacttt acatcctgaa
     2461 cctcggggcc gtggatcgca tcgtgcaaag cgccgccgcg ggctcggtgg atgcccaggc
     2521 cgcccccgct ggggccgcga atgtgcagct ccgcgtggat caaatggaga gtctgggggt
     2581 gtccccccac cagatggcgg agatccggcg cacgcggcaa attcccccga gcatccagat
     2641 cctttcgccg gccgatggca ttgtcattgc gcggaacgta tcgcccggcg tgaaatttga
     2701 caagggcacg gaatggtatc ggatcgcgga cctgcgccgg gtctggatcc tggtcgacgt
     2761 gttcgagaac gaggcccagt acctccagcc cggcaaggtt gtgacggtgt cactccggca
     2821 gcaaaacaag accttccagg ctaaggtaag cccggtccgc ccccagttcg accccgccac
     2881 gcgcaccctc aaggtgcggc tggaggcaga caacctcggg gacatcctcc ggccggacat
     2941 gttcgtggat gtggagctac ccgtcagcac gcctcccatg atctcggtgc ccgtggatgc
     3001 ggtggtggat tccgggctca cgcagaccgt gttcgttgac cggggcgagg gccgcttcga
     3061 accccgccgg gtcgagaccg gcggccactt cggcgaccgg gtggagatcg tcaagggact
     3121 gatggaagga gagcggatcg tggtgtcggg catcttcctg ctcgattcgg agagccggat
     3181 gaagacggtc gcaggggcga ccagccccat gcatgccgcc catgggcagc acgctgacgg
     3241 tgcgatggat
//
LOCUS       DBNGZC010000158         2840 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2164146, whole genome shotgun sequence.
ACCESSION   DBNGZC010000158 DBNGZC010000000
VERSION     DBNGZC010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2164146"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="AC16G0_02990"
     CDS             <1..526
                     /locus_tag="AC16G0_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5098618.1"
                     /translation="GEVVGGAIFAVYLVPTTLLFIPLSVVIRELGLADTRWSLVVTYP
                     TFLVPFATWLLMSYFRTIPAELEESAWIDGCSRLGALVRVLLPTAIPGVLTAALFAFT
                     LSWNEFIYAVMFVSSTAQKTLGPGVVMELVRGDVFYWGELMAGAVLGSVPIAVVYAFL
                     LDYYISGLTAGAIK"
     gene            591..1874
                     /locus_tag="AC16G0_02995"
     CDS             591..1874
                     /locus_tag="AC16G0_02995"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098619.1"
                     /translation="MDTRVKRRHFLKVAGAAGAAYGLSLTGRIPRIARAQERELTILM
                     FSSFVPANDEELRVQAAEFGKQNKMKVTVDFISIPEMAGKHASEVQAQSGHDVSAFEN
                     LQTAMVQDQLIDLDDINEEIIKRWGPWHPIAKDACFLNGHWKAIARMSVAFHATYRED
                     YLGDAGQRAPDSWGDLLQAARALRGKDHPVGFAISQCGDANNSLYQILWGFGGKLATP
                     EGEVAIDSPETEQAIAYVQEMFPLMDREVLAWDNAGNNRFILSGRGSWTLNPASIYMS
                     SKTSNPDLASKLNHYGALTGPKGRFGIGDFYSFGIWKFARNKQAAKDFLRYFYQEENQ
                     NRYIETGGGFNLPAHPHFDGHPVWRRDPKLQGIIGYTKWQKLTGWPAPPDRRAQAVYA
                     TFILPNMFAQVVTGKLKAKEAMQWAARQLRDAGYK"
     gene            1887..2639
                     /locus_tag="AC16G0_03000"
     CDS             1887..2639
                     /locus_tag="AC16G0_03000"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HIR5098620.1"
                     /translation="MAAVQPMDRFYDACISILECVRTQERQNISRAADLVAEKLRKDE
                     LVHVFGTGGHSVMGAMELFWRAGGLAGLNPLFPPGIANLEGHPNTERVLGFAVHVLDY
                     YRVKQGDLLILINVNGINALTIDTALECRRRGVTVIAVTSREFAEGVAPNIPARHPSN
                     KNLCDLADVVIDSHVPPGDAVVEIDGFARRVSSVSTVINTYILQSLAAEVVDRIVKAG
                     GTPEVFYSGNMAGAEVMNAPLFAKYYPRVKHL"
BASE COUNT          527 a          885 c          916 g          512 t
ORIGIN      
        1 aggggaggtc gtgggcggcg cgatctttgc ggtgtacctg gtgcccacca cgctgctctt
       61 catccccctg tcggtggtca tccgggaact gggcctcgcg gatacccgct ggtcactcgt
      121 ggtgacctac ccgacgttcc tggtcccctt tgcgacgtgg ctgctcatga gctactttcg
      181 caccatcccg gcagagttgg aggagagtgc gtggatcgat gggtgcagcc gtctcggcgc
      241 cctggttcgt gtgctcctcc cgacggctat tccgggcgtt ctcacggcgg cgctgttcgc
      301 gttcacgctg tcctggaatg agttcatcta cgccgtcatg ttcgtgagct cgacggcgca
      361 gaagacactc ggaccaggcg ttgtgatgga gctcgtccgc ggggacgtgt tctactgggg
      421 ggagctgatg gcgggggcgg ttctgggctc cgtccccatc gcggtcgtct acgcgttcct
      481 gcttgactac tacatctccg gcctcacggc gggggccatc aagtgacgca ggcccagaag
      541 ggcaaatcgg caagggcagt ggacacggtc gtgacagaag ggaggagacc atggacacac
      601 gagtcaagag acgccacttc ctgaaggtag ccggcgctgc cggcgccgcc tatgggctga
      661 gcctcaccgg gcgcatccca cggatcgcgc gcgcgcagga gcgggaactg accatcctca
      721 tgttcagctc gttcgtgccg gccaacgacg aggagctccg cgtgcaggcg gccgagttcg
      781 gcaaacagaa caagatgaag gtcacggtgg acttcatctc cattccggag atggcgggga
      841 agcacgcctc cgaggttcag gcccagagcg gccacgacgt ctccgccttt gaaaatctcc
      901 agacggcgat ggtgcaggat cagctcatcg acctcgacga catcaacgag gagatcatca
      961 agcggtgggg gccgtggcat ccgatcgcca aggacgcctg cttcctgaac gggcactgga
     1021 aggcgatagc ccggatgagc gtcgcgtttc acgcgaccta ccgggaagac tacctcggcg
     1081 acgcaggcca acgagccccc gacagctggg gtgatctctt gcaggccgca cgggcgctgc
     1141 gcggcaagga tcacccggtc ggcttcgcca tcagccagtg cggggatgcc aacaactcgc
     1201 tctaccaaat cctctggggg ttcggaggca agctggccac acccgagggc gaggttgcca
     1261 tcgattcgcc ggagaccgag caagccattg cctatgtcca ggagatgttt cccctgatgg
     1321 atcgcgaggt tctggcatgg gacaatgccg gtaacaatcg attcatcctc tccggacgcg
     1381 gctcctggac cctgaacccc gccagcattt acatgtcgtc caagaccagc aacccggacc
     1441 tggcttctaa gctcaatcac tacggggcgc tgactggtcc gaaggggcga ttcggcatcg
     1501 gtgacttcta cagcttcggc atctggaagt tcgcccggaa caagcaggcg gcgaaagact
     1561 tcttgcgcta cttctatcaa gaggagaacc agaatcgcta cattgagacc ggcggcggct
     1621 tcaacctgcc cgcccatccc cacttcgacg gccatcccgt ctggcgccgc gatccaaagc
     1681 ttcaggggat catcgggtac accaagtggc agaagttgac aggctggcca gcacccccgg
     1741 atcggcgggc acaggcggtg tatgcgacgt ttatcttgcc aaacatgttc gcccaggtcg
     1801 tcaccggcaa gctgaaggcc aaggaggcga tgcagtgggc ggcacgtcaa ctccgcgacg
     1861 ccgggtacaa ataggaggga ctgaccatgg ccgcagtgca accaatggac cggttctacg
     1921 atgcgtgtat cagcattctg gagtgtgtgc ggacccagga gcgacagaat atttcccggg
     1981 cggccgatct ggtcgcagag aagctgcgca aggatgaact ggtgcatgtg ttcggaacgg
     2041 gcggccactc ggtcatgggg gccatggagt tgttctggcg cgcaggcgga ctggcgggcc
     2101 tgaatcccct cttcccgccc gggattgcca atctggaggg ccatccgaat accgagcgcg
     2161 tgctcggctt cgctgtgcac gtcctcgact actatcgggt gaagcagggg gacctgctga
     2221 tcctcatcaa tgtgaacggg atcaacgcgc tcaccatcga cacggcgctg gagtgccgcc
     2281 gccgcggcgt caccgtcatc gcggtgacct cccgggagtt cgcagagggg gtggcgccga
     2341 acatcccggc ccggcatccg agtaataaga acctctgcga tttggccgac gtcgtcatcg
     2401 actcgcacgt cccgccgggc gatgccgtcg tggagatcga tggatttgct cgccgcgtgt
     2461 cctcggtgtc cacggtgatc aacacctaca ttctccaatc actcgccgcg gaggtggtgg
     2521 atcgcatcgt gaaggccggg ggcaccccgg aggtattcta cagcggcaac atggcggggg
     2581 cggaagtgat gaacgcgccg ctctttgcca agtactaccc acgcgtcaag cacctctaac
     2641 ctgcctgcgg aggggcgcgg gagcggcccc tccgcacacc ttacctgccc tcgatgtggc
     2701 tgatcgatga cttcatccat caggcgcgct aggtgcgcgt ggtcctgaac gccaacatcc
     2761 tgttggtgac caagttccgc cagacgccca ccgaggccga cgaggccgcg cgggtcatcc
     2821 gggccgcagc cggggttgtc
//
LOCUS       DBNGZC010000159         1598 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2360278, whole genome shotgun sequence.
ACCESSION   DBNGZC010000159 DBNGZC010000000
VERSION     DBNGZC010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2360278"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="AC16G0_03005"
     CDS             complement(<1..917)
                     /locus_tag="AC16G0_03005"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF028222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR5098621.1"
                     /translation="MKAFIKAARGIGNMAVGEVPVPVPGPGEALVKIEQSGMCGTEIL
                     VYDDVYRGRNRPVPYPLIVGHEASGMVVELGPGAEGPAPGTRVGIEAVSGCGRCFHCL
                     RGKYNLCPDWHHIGLTRAGALAEFVAVPASTLLPLPDEVSSEAAAFLEPLATVVHTLE
                     RTRPIPGQPAAIIGPGPLGLLHLQALRSAGLCPIIIFGRHGDEKRLDLARTLGADAAI
                     IADRKQAQEYAASVTEGVGMGLVIEAGGSPEAVQTALDIVSGQGTMVTLGIVRATEID
                     ALTVMRKDLTWLGVVAAVRRHFAEGIRLIR"
     gene            complement(921..>1598)
                     /locus_tag="AC16G0_03010"
     CDS             complement(921..>1598)
                     /locus_tag="AC16G0_03010"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR5098622.1"
                     /translation="KRRQTWLGELAASQRDAGRPIHPARLLYELNQTLPEHAIVVTDV
                     GWNKNGAGQQLLTRRPQTFITSGGMATMGYAPAAAIGAKIACPDRPVVALVGDGGLMS
                     ACGALATAVELDAPVLWVLFNNFCYSTIRTMGDAFFKNTYGTKFERRDGTPYNPDFQL
                     LAKAHGMEGRLVEDPDQLSSTLKECLSLNAPCLLEVRTRGDLPMPRTGYWDIADFLTT
                     DAPSKGE"
BASE COUNT          284 a          559 c          472 g          283 t
ORIGIN      
        1 cgaatcagcc ggatgccctc ggcgaaatgc ctccgaacag ccgcgacgac acccaaccag
       61 gtgagatcct tgcgcatgac ggtcagggcg tcgatctctg tggcgcgcac aattccaagg
      121 gtcaccattg ttccctggcc gctcacaata tccaaggcag tctggaccgc ctccgggctg
      181 ccaccggcct cgatgaccaa ccccattcca acgccttcag tgacgctcgc ggcgtactcc
      241 tgcgcctgct tccgatcggc aatgatggcc gcatcggccc ccagcgtcct ggccaagtcc
      301 aggcgcttct catccccatg gcggccaaaa atgatgatgg gacataaccc tgcagagcgg
      361 agtgcctgga ggtgcagcag gccgagaggt cccggcccga tgatcgccgc cggctggcct
      421 gggattggcc tcgtcctttc cagcgtatgg actaccgtag ccagcggctc cagaaatgcg
      481 gcagcctccg atgagacctc gtctggcagc ggaagcaagg tggaggcggg caccgcgaca
      541 aactcggcaa gcgccccggc cctggtgaga ccgatgtggt gccagtccgg gcagaggttg
      601 tacttcccgc gcaggcaatg aaagcaacgc ccgcagccgc tcaccgcttc tatgcccacg
      661 cgggtccccg gcgcaggccc ctccgcgccc ggccccagct ccaccaccat gccggaggcc
      721 tcatgcccga cgatcaacgg atagggcacc gggcggttcc gtcctcgata cacatcgtcg
      781 tagaccaaga tctccgtgcc gcacatgccc gactgttcaa tcttgaccag tgcctcaccg
      841 ggtcccggaa ccgggaccgg cacctcgccc acagccatat ttccaatccc acgcgccgcc
      901 ttgatgaagg ctttcattgc tcactccccc ttgctgggcg catcggtcgt gaggaagtcg
      961 gcgatatccc aatagcccgt cctcggcatc ggcaagtctc ctcgcgtccg cacttcgagc
     1021 aggcaggggg cgttcagact cagacactcc ttcagggtgg aggacaactg gtcgggatcc
     1081 tcgaccagcc gcccctccat gccatgggcc ttcgccagga gctggaaatc cgggttgtag
     1141 ggcgtgccat cgcgtcgctc gaatttggtg ccgtaggtgt tcttgaagaa ggcgtctccc
     1201 atcgtccgga tcgtcgaata acagaagttg ttgaaaagca cccacaacac gggcgcgtcg
     1261 agctccacag ccgtcgccag ggcgccgcag gccgacatca ggcctccatc gccgacgagc
     1321 gccaccaccg gtcgatcggg acaggcgatc ttcgcgccga tggccgcggc gggcgcatac
     1381 cccatggtag ccattccgcc gctggtgatg aatgtctgtg gtctccgagt caagagttgc
     1441 tgtccggccc cattcttgtt ccagcccacg tccgtgacca cgatggcgtg ctccggcagc
     1501 gtctggttca gctcgtagag gagtcgggcg gggtggatgg gcctgcctgc atctctctgg
     1561 ctggccgcca actcgcccag ccatgtctgc ctcctctt
//
LOCUS       DBNGZC010000160         3004 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1968906, whole genome shotgun sequence.
ACCESSION   DBNGZC010000160 DBNGZC010000000
VERSION     DBNGZC010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1968906"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..449)
                     /locus_tag="AC16G0_03015"
     CDS             complement(69..449)
                     /locus_tag="AC16G0_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098623.1"
                     /translation="MNGLLGDTVMRRLPREIVWFLAVTCMLAGCNLFDFGYTPIGDII
                     KNPSQFDGKGVKVRGKIVDVVKVPLLETKMYMLKDDTGQILVITAGDLPAMGSEARTK
                     GIIQNVAILGGQSLGLHLRESERW"
     gene            complement(494..1543)
                     /locus_tag="AC16G0_03020"
     CDS             complement(494..1543)
                     /locus_tag="AC16G0_03020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014653.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HIR5098624.1"
                     /translation="MGDEVRVAAEPLTKFTREVFVRAGFSQEDAALQAEVLVWANLRG
                     VDSHGVLRIPVYLDFIDKGWMKPRAKIQVEKETPATFLIDADYAPGPVVTVFAMTRAI
                     EKARTTGIGWGLIRNTTHQGAMAYYSLMAAREGLAGLAIVCSPPNMAPYGAKAPGLHN
                     SPITIAVPAGRHPPLVLDMATSVAAGGKLPLAVDKGVPIPLGWALTKEGEPTTDARQA
                     KILLPVGGPKGSGLAMTFECLTSLMVANPLLEPSLLGKEQVRRHRQNSVVAAIDIATF
                     TDLDPYRAHVDTLIRELKALPRSAGTEEILVPGEIEDRIHAERTRHGIPLPTGTVEKL
                     RTVGQRLTIPLPTGI"
     gene            1748..2263
                     /locus_tag="AC16G0_03025"
     CDS             1748..2263
                     /locus_tag="AC16G0_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR5098625.1"
                     /translation="MLQSTAATMAIRAALFLALQPSGTLFPVREIAKQTGLPEPNQAK
                     IVPQITSPGLVRAFRGPGGGIALVQSPQSVSLWSIVRAIEGPVEAEWCVLGLHPCSAE
                     TPCPVHPQYARLRTEMQHLLEKTTLALLLRRLRKVGDLDGKAWLASTGDSSRRPYGKT
                     SGKDARDKNRR"
     gene            2278..>3004
                     /locus_tag="AC16G0_03030"
     CDS             2278..>3004
                     /locus_tag="AC16G0_03030"
                     /inference="COORDINATES: protein motif:HMM:NF026042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HIR5098626.1"
                     /translation="MPDASALQVEVPGEGYHKDLISCQVACPVHTDARGYVRAIAAGR
                     FEEAYLIARGPNPFASICGRICGAPCEIACRRGKVPRVDADGHFVSIDRPIAIRALKR
                     YACEQAGPDVRALDQVLETVRSYVPQVAADADEMAALLKSLTTGQFTPADGQRVAIIG
                     SGPAGLSAAHDLALLGFRPVVYETEPVASGMLAVGVPSYRLARELIRREVAVIEALGV
                     EIRCGGTVGQDGSFAGLRREYGAV"
BASE COUNT          493 a         1043 c          918 g          550 t
ORIGIN      
        1 cttggcagga atcgacacat ctcacctcgg actcagcggc acggcgagtc tctcgtagcc
       61 ccatcggatc accaccgctc ggattccctg agatgtaacc ccaggctttg cccgccgagg
      121 atggcgacgt tctggatgat tcccttggtc cgggcctcgg atcccatggc ggggagatcc
      181 ccggcggtga tgacgagaat ctgcccggtg tcatccttca acatgtacat cttggtctcg
      241 agaagaggaa ccttcacgac atccacgatc tttccccgga ccttgacacc tttgccatcg
      301 aattggctcg gattcttgat gatgtcgccg atcggggtat agccgaagtc aaatagattg
      361 cagcccgcca gcatgcacgt gaccgctaga aaccagacga tctccctcgg aagtctccgc
      421 atcaccgtgt cccccaagag gccgttcatg ccgacggatc cagatccaca tgaaagccgg
      481 aagaactccc tggctatatg ccggtaggga gtggaatcgt gagccgttga cccaccgtgc
      541 gcagcttctc caccgttccc gtgggaagcg gaatgccgtg gcgcgttcgc tcggcatgga
      601 tccggtcctc gatctctccg gggacgagga tctcctcggt gcctgcgctc ctggggagag
      661 ccttgagctc ccggataaga gtgtccacgt gtgctcggta cgggtcgagg tcggtgaacg
      721 tggcgatgtc gatcgccgcc accacgctgt tctgccgatg gcggcgaacc tgttccttgc
      781 ccagcagcga aggctcgagc aacgggttcg ccaccatgag gctggtgagg cattcgaacg
      841 tcattgcgag cccggagcct ttcggccccc cgacagggag cagaatctta gcctgcctgg
      901 cgtccgtggt cggctcgccc tccttggtca aggcccaccc cagcggtatg gggacgccct
      961 tgtcgacagc caaggggagt ttgcctccgg cggccacgct cgtcgccatg tccagcacga
     1021 gaggtgggtg ccgccctgcc ggcacggcaa tcgtgatggg gctgttgtga agaccgggag
     1081 ccttggcccc gtacggggcc atgtttggcg ggctgcatac gatcgccagc cccgccagcc
     1141 cttcgcgtgc cgccatcagg gaatagtacg ccatggcccc ttgatgcgtg gtgttccgga
     1201 tgagccccca gccgattccc gttgtgcgcg ccttttcgat ggcccgggtc atggcaaata
     1261 cggtcacgac cggccctggc gcatagtcgg cgtcaatcag gaacgtcgcc ggtgtttcct
     1321 tctcgacctg gatcttcgcc cgaggcttca tccagccctt gtcgatgaaa tccagataca
     1381 cggggatgcg cagcaccccg tgggaatcca cgccccggag attcgcccag accagcacct
     1441 cggcctggag cgccgcgtcc tcctgcgaga agcccgcccg gacgaagacc tcccgggtga
     1501 atttcgtcag cggctccgca gccacgcgca cctcgtcccc catctgttcc tccccttggc
     1561 ctgcgagttc gccccacccg acctgaaacg cggggcccat ggtccgtcat ccagtccccg
     1621 cagtcaaggg ggcgccgctc ggcccgccca aggcctcggg tcatcttttc tcgacgggtc
     1681 ccaaaaacac ttgacagaaa atccattctg gattaaatgg tccacaattc ctctatggag
     1741 gtccgggatg ctccagagca ccgccgcaac gatggccatc cgagcggccc tgttcctggc
     1801 gctgcaaccc agcgggacgc tcttcccggt tcgcgagatc gccaagcaga ccggactccc
     1861 cgagcccaac caggccaaga tcgtgcccca aataacctcc cccggcttgg ttcgcgcttt
     1921 ccgcggtccc ggcggcggca ttgccctcgt ccagtccccc cagtccgtga gcctctggtc
     1981 catcgtccgc gccatcgagg ggcccgtgga ggccgagtgg tgcgtgctcg gtctgcatcc
     2041 ctgctcggcc gaaacgccct gtcccgtcca tccccagtac gcccgcctcc gcaccgagat
     2101 gcagcacctg ctggaaaaaa cgaccctcgc tctcctcctg cggcgactgc gcaaggtggg
     2161 cgacctcgac gggaaggctt ggttggccag caccggcgac tcttctcgac gcccttatgg
     2221 gaagaccagc ggcaaggatg cacgagacaa gaatagaagg tagcggcgag gcctgctgtg
     2281 cctgacgcga gcgcgctcca ggttgaagtt cccggtgagg gctaccacaa ggacctgatc
     2341 tcctgccagg tggcctgccc tgttcacact gacgcccgcg gctacgtgcg tgcgatcgcg
     2401 gcgggacgct tcgaggaagc ctacctgatc gccaggggac ccaacccctt cgcctccatc
     2461 tgcggtcgga tttgcggcgc cccctgcgag attgcctgcc ggcgcggcaa ggtgccccgg
     2521 gtggacgccg acggccactt cgtctccatc gatcgcccca ttgccatccg cgccctgaag
     2581 cgctacgcct gcgagcaggc cggacccgat gtccgggctc tggaccaggt cctcgaaacg
     2641 gtccggtcct atgttcccca ggttgcggcc gacgccgacg agatggcggc cctcttgaag
     2701 tccctgacca ccggacagtt cacgcctgcc gacgggcagc gcgtggccat catcggttcc
     2761 ggaccggccg gcctctcggc cgcccacgac ctcgcccttc tgggtttccg gcccgtcgtc
     2821 tacgagaccg agcccgtggc atcgggaatg ctggcggtag gggtcccctc ctatcgcctc
     2881 gcgcgagagc tgatccgccg cgaggtcgcg gtgatcgagg cgctgggcgt cgagatccgg
     2941 tgcggcggta ccgtgggcca ggacggttct ttcgctggcc ttcgccggga gtacggggcc
     3001 gtga
//
LOCUS       DBNGZC010000161         2497 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_306397, whole genome shotgun sequence.
ACCESSION   DBNGZC010000161 DBNGZC010000000
VERSION     DBNGZC010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_306397"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..1364
                     /locus_tag="AC16G0_03035"
     CDS             51..1364
                     /locus_tag="AC16G0_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR5098627.1"
                     /translation="MAFLFLFLAYLGVPVPFALSAGVFVATMLTQISMQSIVGQLLNG
                     LDSEALLAVPFFLLVGELMTSADVTRRMIDLAQTIVGHFKGGLAQVVTVFSMFFAGIS
                     GSSTADVAVLTKTIAPEMDREGYDAPFTAALIASAATIANLIPPSIMAVVYGATGSVS
                     IAGLFLGGVAPGVFIGFGLMIYCFLFGPVGIRRRRSSFGEFSEAAKRAALPLVIPVII
                     MGGILTGWFTPTEAGMAAVAYIVLVVIPFLRPAHIKKLPRDFVHAGLLYSIPMAAVAG
                     ASAFGWMLAYLRGPDVVASWIEHYAGTDGRTIMLLLAALFIIVGDFVDAVPAIIIFMP
                     ILLRLQELGNINPVHLGVVIVTTMAFGLITPPYGLTLLMASKFCNVRFSKALVRSLPL
                     YVVFLAAILFTIFFPEIVLWLPKVLLPESVGCFKNPAGTGYICPS"
     gene            complement(1410..>2497)
                     /locus_tag="AC16G0_03040"
     CDS             complement(1410..>2497)
                     /locus_tag="AC16G0_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013645.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GltB/FmdC/FwdC-like GXGXG domain-containing
                     protein"
                     /protein_id="HIR5098628.1"
                     /translation="YQDAQRFCERMTACFAEDEGRRLSLESLGLLIDRRYPTGPLRRS
                     RLISMFDDTLSALFQAIRQQPSRGFGFHEHGRPLPRPEADGQVMVVDARGFPPEGPVS
                     LARELVRLVEHGFSWLVVAEARGHRFIANGLGPMTQGVRIDVFGSSGDYLGSGIDGAE
                     VVVHGSGQDQLAQIMKAGRLVVHGDVGQTFMYAAKGGEVFVLGNAAGRPLINAVGSPR
                     VVINGTCLDYLAESFMAGDPLNGGGFVVLNGIRFDADGRLQDLETPYPGGNLFSLASG
                     GAVFIRDPGAKLGEDQLNGGRFGTLSPAHWERILPLLLENERLFGIPITRLLTVGSET
                     LPPERVYRIVIPAKVRALQAEEAWVSH"
BASE COUNT          399 a          815 c          793 g          490 t
ORIGIN      
        1 gggttcacca tatgaacaca gccgtcccgc cgggcctcat cgtcttcctg atggcatttc
       61 tctttctgtt cctcgcgtac cttggcgtgc cggtcccgtt cgcgttgtct gccggggtat
      121 tcgtcgcaac gatgctgacg cagatcagca tgcagtcgat cgtgggccag ctactgaacg
      181 gattggactc cgaagcgctg ctcgcggttc cctttttcct gctcgtcggc gagctgatga
      241 cctcggccga cgtcacacgg cgcatgatag atctggcgca gacgatcgtc ggccatttca
      301 agggtgggct ggcgcaagtc gtgacggtct tcagcatgtt cttcgcaggg atttcggggt
      361 cttcgacggc agacgtggcg gtgctcacaa agaccatcgc tcccgagatg gaccgcgaag
      421 gttacgatgc tccattcact gccgccctga tcgcgtctgc cgcgacgatt gccaatctga
      481 ttccgcccag catcatggcc gtcgtgtacg gcgcaacggg cagcgtgtcc attgcggggc
      541 tgttccttgg cggtgtagcc cccggcgtgt tcatcggatt cggcctgatg atctactgtt
      601 tcttgttcgg tccggtcggg atccgaagaa ggcggtccag cttcggtgaa ttcagtgaag
      661 ccgcgaagcg ggccgccctt ccgctggtca ttcccgtcat catcatgggc ggaatcctta
      721 ccggctggtt caccccgacc gaggcgggga tggcggcggt ggcttacatc gtcctggtcg
      781 tcattccgtt cttgcggccg gcccatatca agaagctgcc gcgcgatttc gtgcacgcgg
      841 gcttgctgta ctcaattccc atggcggcgg tggctggcgc ctcggcattc ggctggatgc
      901 tggcatacct ccggggtccg gatgtggtgg cgagctggat cgagcactac gcgggaacgg
      961 atgggcggac gatcatgctg ctgctggcgg cactgttcat catcgtcggc gactttgtgg
     1021 acgcggtgcc ggcgatcatc atcttcatgc ccatccttct ccggctccag gagctcggaa
     1081 acatcaatcc ggtccacctc ggggtggtca tcgtcacgac catggccttc ggattgatca
     1141 ccccgccata cggattgacg ctgctgatgg cctcgaagtt ctgcaacgtc cgcttttcaa
     1201 aggcgctcgt ccgctcgttg ccgctgtacg tggtgttcct ggccgcgatc ctgttcacga
     1261 tcttcttccc ggaaatcgtc ctctggcttc ccaaggtgct gctgccggag tcggtgggct
     1321 gcttcaagaa cccagccgga accggataca tctgcccttc gtgacggatc ggcgaggccg
     1381 cagggtaggt cggttcctag acaggttctt cagtgactca cccaggcctc ctcggcctgg
     1441 agggcgcgga ccttggcggg aatgacgatc cggtaaacgc gctcgggcgg aagggtctcg
     1501 cttcctactg tcaacagccg ggtgatgggg atgccgaaga gacgctcatt ctccaggagc
     1561 aacggcagga tccgctccca gtgggccggg ctgagcgtgc cgaaccggcc accgttgagc
     1621 tgatcctcgc cgagcttcgc ccccggatcc cggatgaaca ccgcgccgcc tgacgcgagg
     1681 gagaagagat tgccgccggg gtagggggtc tcgaggtcct gcaggcggcc gtcggcatcg
     1741 aaccggatgc cgttcaggac cacgaacccg cctccgttca gcggatcgcc ggccatgaag
     1801 ctctcggcga gatagtccag gcaggtgccg ttgatcacga cccggggtga tcccacggcg
     1861 ttgatgagcg ggcgtccggc ggcgttcccc aggacgaaca cctctccccc cttcgccgcg
     1921 tacatgaagg tctggcccac gtcgccatgg accaccagcc gtcccgcctt cattatctgg
     1981 gcgagctggt cctgtcccga cccgtgcacg acgacctcgg cgccgtcgat cccggagccc
     2041 aggtagtccc ctgaggagcc gaacacgtcg atccggacgc cctgggtcat cggtcccagg
     2101 ccgttggcga tgaagcggtg cccgcgcgcc tccgccacaa ccagccaact gaacccatgc
     2161 tcaacgaggc gcaccagctc ccgcgccagg gaaaccggcc cctccggcgg gaagccccgg
     2221 gcatccacca ccatcacctg tccatcggcc tcgggacgcg ggagcggccg gccatgctcg
     2281 tggaatccaa accccctgga gggttgctgc cggatggcct ggaagagggc cgaaagcgtg
     2341 tcatcgaaca tggagatcag gcggctccgg cgaagcgggc ctgtcgggta gcggcgatcg
     2401 atcaggaggc cgaggctctc caggctcagc cgacgcccct cgtcctccgc gaagcacgcg
     2461 gtcatccgct cgcagaaccg ttgggcgtcc tgatagc
//
LOCUS       DBNGZC010000162         1574 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_501606, whole genome shotgun sequence.
ACCESSION   DBNGZC010000162 DBNGZC010000000
VERSION     DBNGZC010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_501606"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="AC16G0_03045"
     CDS             complement(<1..181)
                     /locus_tag="AC16G0_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098629.1"
                     /translation="MTRPVDTEVTMRPGRTDMFPATSQVLARPSTSLAPRIVVLGGGF
                     AGVTTALELAKRCAGV"
     gene            complement(339..>1574)
                     /locus_tag="AC16G0_03050"
     CDS             complement(339..>1574)
                     /locus_tag="AC16G0_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009457071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HIR5098630.1"
                     /translation="EQLLDGWRNAHPNPGRAPAWIQHLVLAADQFVVRRPLPDDPGGM
                     SAIAGYHWFGDWGRDTMISLPGLLLSTGRPEIARRILTTFARFVDQGMLPNRFPDAGE
                     APEYNTVDATLWYFEAIRAYHAMTGDTTLLDELYPVLGDIIRWHRKGTRYGIAEDPQD
                     GLLRSGEPGVQLTWMDAKIGDWVVTPRTGKAVEINALWSNALCAMATFARHLGRPAES
                     YDALAARVLEGFGRFWNDAVGYCFDVIDGPDGNDPALRPNQILAVSLPESALPPERQR
                     QVVDACARSLLTSIGLRSLAPGHPQYRGRYLGGPRERDAAYHQGPVWGWLLGPFALAY
                     LRVYKDGEGARRFLEPMAQHLAEYGMGSIAEIFDGDSPFIPRGCCAQAWSVSETLRAW
                     MEIEGRIGRSDPGVAPAKG"
BASE COUNT          267 a          517 c          523 g          267 t
ORIGIN      
        1 ggactcccgc gcagcgtttg gccagctcca gcgcggtggt gaccccggcg aacccgcctc
       61 ctaggacaac gatccggggc gccagcgagg tagacggccg ggccagcacc tgactggtgg
      121 ccgggaacat gtcagttcgg cctggtctca tcgtgacctc ggtgtctaca ggtcgtgtca
      181 tgttgggctc gacggtgacg cgcatcctcg tttccccttc acttcctaag atactcggca
      241 ggggtcgagc gattcggttt cttgcatacg agggtgcggc ccaggtaggc gtcgtgtgag
      301 gctaccccgc tagcaggtcg tgggcaagcg acgtcccatt atcccttagc cggggccaca
      361 cctgggtcag accggccgat ccgtccctca atctccatcc aggctcgcag ggtctcggag
      421 acgctccagg cttgggcgca gcagcccctg gggatgaagg gcgaatcccc gtcgaagatt
      481 tcggcgatgc tccccatccc gtattcggcg agatgctgcg ccattggctc gaggaaccta
      541 cgagccccct ccccgtcctt gtagacccgc agataggcca gggcgaatgg ccccaggagc
      601 catccccata cggggccctg gtggtaggcg gcgtcgcgct ccctcggtcc gcccaggtaa
      661 cgcccccgat actggggatg gccgggtgcg agactcctga ggccaatgga agttaggaga
      721 cttcgcgcac acgcatccac cacctgccgc tgccgctccg ggggcagagc actttccggg
      781 agggatacgg caaggatctg attcgggcgg agcgctggat cgttcccatc gggaccgtca
      841 atgacgtcga aacagtatcc gaccgcgtcg ttccagaatc gaccgaaccc ctctagcacc
      901 cgggcggcca gggcgtcata ggactcggcc ggccggccga ggtgcctcgc gaatgtcgcc
      961 atggcacaga gtgcgttgga ccagagcgcg ttgatctcca cggctttgcc tgtccgcggc
     1021 gtgacgaccc aatcgccgat cttggcatcc atccacgtga gctggacacc tggctccccg
     1081 gaccggagca ggccatcctg cgggtcctcg gcgatgccgt agcgggtccc cttccgatgc
     1141 cagcggatga tatcccccag gaccggatag agctcatcga gcagcgtggt atcacccgtc
     1201 atcgcatggt aggcgcggat ggcttcgaag taccagaggg tggcgtccac ggtgttgtac
     1261 tcgggcgctt cccccgcgtc cgggaaccga ttgggaagca ttccctggtc cacgaagcgg
     1321 gcaaaggtcg tgaggatccg gcgggcaatc tccgggcgtc cggtcgagag aaggaggccg
     1381 ggcaggctga tcatcgtgtc gcgcccccag tcaccgaacc agtggtagcc ggcgatggcc
     1441 gacatccccc ccggatcgtc ggggagcggg cgacggacga cgaactgatc cgcggccagg
     1501 acaaggtgct ggatccaggc aggcgcccgg ccggggttgg gatgggcatt ccgccagcca
     1561 tccagcagtt gctc
//
LOCUS       DBNGZC010000163         2461 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_599613, whole genome shotgun sequence.
ACCESSION   DBNGZC010000163 DBNGZC010000000
VERSION     DBNGZC010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_599613"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..114
                     /locus_tag="AC16G0_03055"
     CDS             <1..114
                     /locus_tag="AC16G0_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007331623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HIR5098631.1"
                     /translation="VLNAAAWTYVAATLTSILTLLYFLFRSGLLGGRKSEE"
     gene            827..1027
                     /locus_tag="AC16G0_03060"
     CDS             827..1027
                     /locus_tag="AC16G0_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098632.1"
                     /translation="MSDHEPLHRLVEDLIDVMQLQAKELEKLVVHIEQAVGRLPEESQ
                     LAVIASELSELKQRAQRLGAST"
     gene            1140..1601
                     /locus_tag="AC16G0_03065"
     CDS             1140..1601
                     /locus_tag="AC16G0_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5098633.1"
                     /translation="MSVGRICTRYVDLADPEETVQAAARRMLERKVGTVVILDPAQRP
                     VGLLTDRDLVLRVLAPGQDPRQTSVGEVMTTEPKTVTEGTPIEQALALMRSGAFRRLP
                     VVGGDGTLVGLVSLDDILSLLAEELREVGTLVEREMPSGTENTRRRGRPTD"
     gene            1685..2137
                     /locus_tag="AC16G0_03070"
     CDS             1685..2137
                     /locus_tag="AC16G0_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5098634.1"
                     /translation="MMKPVVTVGRMTSLSEVARIMVDHRIGCVLVVDEQGKLCGIVTE
                     TDFAAKERGVPFSIHILPQVFSQLIPREAITRLQEAARTATAREIMIGEVITATEETP
                     VAEVAGQMLRYNIEHIPVVHNGVPVGIVTRHDLLRMIVQQAEHEGGVR"
BASE COUNT          426 a          797 c          857 g          381 t
ORIGIN      
        1 gtcctgaacg ccgcggcctg gacctacgtc gccgccacgc tgaccagcat cctgacgttg
       61 ctctacttcc tgttccgctc cggcctgctc ggcggccgca agagcgagga gtagccagtt
      121 ttcgggccca ggggcgatcg ccagatgggg tggtggtgac acgctccggg tgtgggtcca
      181 gggagcgttg tacagccggc gcgagcttga gcccgacccg cccttgcaag attccccccg
      241 ctggcacggg cgccgggacg gacaaacggc gatggacctg tgaggctcgg gaggcgcgat
      301 tagggaatct cggcatccgg aagagtgcat ccaggccgtc ccggcaggga tcgtcgaaca
      361 gatcgaagca cagatccaga ggcgtcatgg cggccaggtg agcggcctcc gggtggtcgt
      421 gcgggtgacg gcctggcctt gcagggccgc tcccgcaccc actactctcg atcgaacgaa
      481 tcgagaggac tggcgcttgt caatccatcg ctcggccgat acgctaatgt tagtctcgcg
      541 ccgcttgtga gaggcggtgc gtctccggct ctggtggccc cgccgtgcaa cccttcgcac
      601 ggacggggtt ttgtcgttct cccccccacg gtccgaggtg agggccggag ggcgcgccgg
      661 agatgaggac accggatctt gagggggaga tgtcggacgc gaaagcccac gcctcctccg
      721 attcaggccg cctcactccg ggcgacgtga tagccaacgc gcccaccggg accgcgcggc
      781 gagcggggcg ggaatcaagc cattcggatc gaaccggagg tgccccatgt cggatcacga
      841 accgctccac aggctcgtcg aggacctgat cgacgtgatg cagctccagg ccaaggagct
      901 cgagaagctg gtcgtccaca tcgaacaggc cgtcggtcgc ctgcccgagg agagccagct
      961 cgccgtgatc gcctcggaac tgagcgaact gaagcaacgg gcccaacggc tgggggcctc
     1021 gacctgagcg cggatggccg ggatcacggg actcggggcg atctcgcccc cggttgcggc
     1081 gcctcactcc ggtcaggccg acgggggggg cgtaaccctg ggttgggaag gagggactaa
     1141 tgtcggtcgg ccgcatctgc acgcggtacg tggacctcgc cgacccggag gagactgtcc
     1201 aggccgccgc ccggcggatg ctggagcgga aggtcggcac cgtggtcatc ctcgacccgg
     1261 cccagcgacc ggtcggcctg ctcaccgatc gggatctggt cctgcgggtc ctggccccgg
     1321 gccaggaccc gcgccagacc tccgtcggcg aggtcatgac aacggagccg aagacggtca
     1381 cggagggaac gccgatcgag caggccctgg cgttgatgcg gtccggcgct tttcgccgtc
     1441 tgccggtcgt cggtggcgac gggaccttgg tgggcctggt cagcctcgac gatatcctca
     1501 gcctcctggc cgaggagctc cgggaggtcg ggaccctggt cgagcgggag atgccttcgg
     1561 gcaccgagaa cacccggcgc cgagggcgcc caaccgactg ataagggggg cactgtctcg
     1621 agcggatctt gatgcgcttt cgaggatgtg gaaccggagc caagcgatga gagcccgaga
     1681 gattatgatg aaaccggtgg tgaccgtcgg gcggatgacc agcctgtccg aggtcgcccg
     1741 gatcatggta gaccaccgca tcggctgcgt ccttgtggtg gacgagcagg gcaaactctg
     1801 cggcatcgtc acggaaacgg acttcgccgc caaggagcgc ggcgtgccct tctcgatcca
     1861 catcctgccc caggtcttca gccaattgat cccccgagag gccatcactc gtctgcagga
     1921 ggcggcacga acggcgacgg cccgggagat catgatcgga gaggtgatca ccgccacgga
     1981 ggagacgccc gtggccgagg tggcggggca gatgctccgc tacaacatcg aacacatccc
     2041 ggtggtgcac aatggggtgc cggtcgggat cgtcacccgc cacgacctgc tgcggatgat
     2101 tgtccaacag gccgagcacg agggaggcgt ccggtagcca tcctccaccg gtcctgatgc
     2161 cgctggcccc agttttctcc gggtcggatc ggaatcaggc catcgtccag gagatcacca
     2221 tgagcaagcg cacccacggc aagggtcgtc aggcccaacc ctcacccccg agccgagaag
     2281 gcgtgaccgc ccccgctcgt cccgtagcca ggccgggacg gcagaccgct gcgccgcagg
     2341 ctgcgccgtc ggctctcaga gggtgtacgg aaagtagttc gagaaatctc aaggggttac
     2401 atcgcattcc gggccgctga tgcgactcgt aacctcggtt ttttcagcga ttcgcggacg
     2461 c
//
LOCUS       DBNGZC010000164         1400 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1774363, whole genome shotgun sequence.
ACCESSION   DBNGZC010000164 DBNGZC010000000
VERSION     DBNGZC010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1400)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1774363"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1400)
                     /locus_tag="AC16G0_03075"
     CDS             complement(<1..>1400)
                     /locus_tag="AC16G0_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR5098635.1"
                     /translation="EQALEPLADLLLADQDSVDPVIEAAVFVNTEKGVDSVEKALAGA
                     RDIIAERVAEDQTARTRMRDLYSRKATVKSKVKTDKEAEGAKFRDYFDWSEPLATIPS
                     HRLLAIRRGEAESILSVRITPPEEEALALLEPLFVTGKGPAADQVRQAVQDGYKRLLG
                     LAMETEIRLESKKKADADAIRVFVENLRELLLAPPLGQKNVLAIDPGFRTGCKVVCLD
                     RQGKLLHDEVIYPSQSEHRLSQAAQIVRELCERYQIEAIAIGNGTAGRETEAFVRGLG
                     LPASISILMVNESGASVYSASDVAREEFPDQDVTVRGAVSIGRRLMDPLAELVKIDPK
                     SIGVGQYQHDVDQAALKRSLDDVVMSCVNRVGVEVNTASRQLLTYVSGLGPQLAANIV
                     GYRNENGPFRTRTDLRKVSRLGPKAFEQAAGFLRIRDSQNPLDASAVHPESYSIVDAM
                     ARDVNCSVQDLMREAGL"
BASE COUNT          234 a          473 c          421 g          272 t
ORIGIN      
        1 aggccggcct cccgcatcag gtcctgcacc gagcaattca catcccgggc catggcgtct
       61 acgatggaat agctttccgg gtgaaccgcg cttgcgtcca ggggattctg cgaatcccgg
      121 atgcgcagga aaccggcagc ctgttcgaag gccttgggcc ccagccgtga caccttcctg
      181 agatcggtgc gtgtgcggaa cggcccattc tcgttgcggt agcccacgat gttggccgcc
      241 agttgcggtc ccaggccgga cacatacgtc aagagctgac ggctggcggt gttcacctcg
      301 acccctaccc tgttgacgca gctcatcacg acatcatcca gacttcgctt gagggcggcc
      361 tgatcgacgt catgctgata ttgccccacc ccgatggact tgggatcgat cttcaccagc
      421 tccgccagag gatccatcag ccggcggccg atggacacgg cgccgcgcac ggtcacgtcc
      481 tgatccggga attcctcgcg cgccacatcg gaggccgagt agaccgaggc gccgctctcg
      541 ttgaccataa ggatggagat cgaggccggc agacccagcc cgcggacgaa ggcctcggtc
      601 tcccgcccgg cggtgccgtt cccgatggcg atggcctcga tctggtatcg ttcgcaaagc
      661 tcccggacga tctgcgccgc ctggctcaat cgatgttcgg attgactcgg atagatgacc
      721 tcgtcgtgta ggagcttgcc ctggcgatcc agacacacca ccttgcagcc ggtgcgaaag
      781 ccggggtcaa tcgccagcac gttcttctga cccaagggcg gcgccagcaa cagctcccgc
      841 aggttctcca cgaagacccg gatggcgtcc gcgtcggcct tctttttgga ttccagccgg
      901 atttcagttt ccatcgccag gcccagcagc cgtttgtacc cgtcctgaac cgcctggcga
      961 acctggtctg ccgccggccc cttgccggtc acgaataacg gctcgagcaa tgccagggct
     1021 tcctcctcgg gcggggtgat ccgcacgctc aggatggact cggcctcgcc gcggcggatg
     1081 gccagaaggc gatgggacgg gatcgtggcc agcggttcgc tccaatcgaa gtagtccctg
     1141 aacttcgccc cctccgcttc cttgtcggtc ttgaccttgg acttgaccgt ggccttccga
     1201 gaatagagat cgcgcattct ggtccgggcc gtctgatcct cggcgacccg ttctgcaatg
     1261 atgtcgcgtg ccccggccag cgccttctcg accgagtcga cgcctttctc cgtattcacg
     1321 aagaccgccg cttctatcac gggatcgacg ctgtcctggt cggcgagaag tagatcggcc
     1381 aggggttcca aggcctgctc
//
LOCUS       DBNGZC010000165         1945 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2557336, whole genome shotgun sequence.
ACCESSION   DBNGZC010000165 DBNGZC010000000
VERSION     DBNGZC010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2557336"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="AC16G0_03080"
     CDS             <1..614
                     /locus_tag="AC16G0_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098636.1"
                     /translation="GRISLLVGVCSVLLGGVMGSALGLVAGYSGGRIENVIMRFVDIL
                     MAFPSLLLGLMVLAVLGNGLFKMILAIGIMLSPAFARVMHASALSLKKREFVEGARSV
                     GAGPIRIVALHILPNVMGEVVVLGSLWTASAIRTEANLSFIGLGVSPPTPTWGNMIRE
                     GTPHLLNAPWLSIFPGLAILVAVLGFNLLGDGLRDVLDPHLRE"
     gene            630..>1945
                     /locus_tag="AC16G0_03085"
     CDS             630..>1945
                     /locus_tag="AC16G0_03085"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR5098637.1"
                     /translation="MSATAALVAFVRSLDPARLSDRVLHQASRSVLDLMGVAIAGTCT
                     PMTQISARFGYDHFAAGKCTVIGSRRPLCVTGAAWVNCNAASALDLDDGHRMAMGHPG
                     AAVIPTALAMAETTGATGREFLAAVVAGYEMAVRASVARVPWYKDRLYSSGIWGVLGA
                     AVAAGKLLGFDEATLQSAIGNAVSHGPFPPGGLQANYSMVKEVIGWAGMTGCAAALLA
                     QQGFLGPEDVMDYSGRWDTARLVDGLGDPERYAILGSYFKPYAVCRWAHSSVDAVLDL
                     AGRHSLRPEEIQKILVETFYEVTRLVNYAPRNAIAAQFSIPFALAIALLRHRVEPEDV
                     SQENLEKPEVLELARKVEVVVDQEINSQFPAKTIARVTIHTVRGAFQASVEYPRGNPE
                     NPMSDDDLKAKFRSLTTKVVGEKACGRLQAAIFDLPRTPDVTSLTQL"
BASE COUNT          301 a          619 c          672 g          353 t
ORIGIN      
        1 cgggacggat ctcgctattg gtgggcgtat gttccgtcct gctgggtggg gtgatgggct
       61 cagcgctggg actcgtggcg ggctattctg gaggcaggat cgagaacgtg atcatgcggt
      121 tcgtggacat cctgatggcg tttcccagtc tgctcctggg gctcatggtc ctggcggtcc
      181 tcggtaatgg gttgttcaag atgatcttgg cgattggcat catgctgtct cccgcgttcg
      241 ccagggtgat gcatgcctct gccctgtcgc tgaagaaacg cgaattcgtc gagggggccc
      301 ggtccgtcgg ggccggcccg atccgcatcg tggccctgca catcctgccc aacgtcatgg
      361 gcgaggtcgt ggtgctcggc agcctctgga cggcctccgc cattcggacc gaggcgaacc
      421 tcagcttcat cggattgggg gtgtcgccgc ccacgcccac ctggggcaac atgatccgcg
      481 aggggacccc gcatctgctc aacgcgccct ggctgtccat cttcccgggc ctggccatcc
      541 tggtggccgt gctcggcttc aacctgctgg gggacggcct gcgcgatgtg ctcgatccac
      601 acctcaggga gtagctaggg ggggaaaacg tgtccgcaac ggcagcattg gtcgcattcg
      661 tccgcagcct ggacccggcc cggttgtccg atcgggtgct gcaccaggcc agccgctctg
      721 tcctggacct catgggcgtc gccatcgccg gcacgtgcac acccatgacg cagatcagcg
      781 cccgcttcgg ctacgaccac ttcgccgccg ggaagtgcac cgtgatcggc tcgcgccgac
      841 ccctctgtgt cacgggagcg gcgtgggtca actgcaacgc cgccagcgcc ttggacctgg
      901 atgatggcca ccgcatggcc atggggcacc cgggggccgc ggtcatcccg acggctctgg
      961 ccatggcgga gacgacgggg gccacggggc gggagttcct ggccgccgtc gtggcggggt
     1021 acgagatggc cgtccgggcc agcgtggcac gcgtcccctg gtacaaggat cgcctgtact
     1081 ccagcgggat ctggggtgtc ttgggcgccg ccgtggccgc cggcaagctc ctgggattcg
     1141 acgaggcgac gttgcagtcc gccataggca acgcggtctc ccacgggccc tttccgcccg
     1201 gcgggctgca ggcgaattac agcatggtca aggaggtcat cggctgggcc ggcatgacgg
     1261 ggtgcgcggc ggccttgctg gcgcagcagg gctttctcgg acccgaggat gtgatggact
     1321 attccggccg ctgggacacc gcccggttgg tggacgggct gggggacccg gagcgatacg
     1381 ccatcctcgg ctcgtacttc aaaccctacg ccgtctgtcg ctgggcgcat tcctcggtgg
     1441 atgcggtgtt agacctggca gggcgacaca gcctgcggcc cgaggagatc cagaagatcc
     1501 tggtggagac tttctatgag gtcaccaggc tggtcaacta tgctccacgc aatgccatcg
     1561 ccgcccagtt cagcatccca tttgccctgg cgatcgccct gctgcgacac agggtcgagc
     1621 cggaggacgt gagccaggag aacctggaaa agcctgaggt cctggagctt gcccggaagg
     1681 tcgaggtggt cgtggaccag gagatcaaca gccagttccc cgccaagacc atcgctcgtg
     1741 tgacgatcca caccgtgcgc ggagcttttc aggccagcgt ggagtaccct cgggggaacc
     1801 cggagaatcc catgagtgat gacgacctga aggccaagtt ccgctcgttg acgacgaagg
     1861 tcgtgggcga gaaggcctgc gggaggctgc aggccgccat cttcgatctg ccgcgtacgc
     1921 cggacgtcac ctcgctcacg caact
//
LOCUS       DBNGZC010000166         1634 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2164961, whole genome shotgun sequence.
ACCESSION   DBNGZC010000166 DBNGZC010000000
VERSION     DBNGZC010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2164961"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..1218
                     /locus_tag="AC16G0_03090"
     CDS             175..1218
                     /locus_tag="AC16G0_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011431136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HIR5098638.1"
                     /translation="MTTQSFPTPPAPGISPLQGLTESLPSDKARTRILVVDDSPLDLQ
                     VLEDLLTSEGYLVTSAQDGEEALAKVAVEPPDLILLDVVMPKLDGYEVCRRLKSDGRT
                     ILIPVVMITSLQATHERIKGIEAGADEFLSKPFNRQELMTRAQSLLKLKRHTDELESA
                     ETVLFTLALSVEAKDPYTEGHCDRLARFSVALGRGLDLPPEYLKALHRGGILHDVGKI
                     GIPDAILLKPGPITETERVVIQTHPSIGERICAPLKSLRLVLPIIRHHHERWDGSGYP
                     DGLAGEAIPVTARILQVADLYDAITTQRPYKPAYSPERAFEIMREETAKGWWDGRLIE
                     TFIALITQTRKMQ"
     gene            complement(1292..>1634)
                     /locus_tag="AC16G0_03095"
     CDS             complement(1292..>1634)
                     /locus_tag="AC16G0_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098639.1"
                     /translation="DKTGFDGVKYRRPEPLDITTLLNRDSQIHVNQVLRFAPAWPEVE
                     RQIAFYGSESDRGKILQKFGLPEKREQVGPGGESEVWWYYTDGVSYWFTGGKLTKTQE
                     FQPVPAATPSR"
BASE COUNT          306 a          542 c          503 g          283 t
ORIGIN      
        1 cttcgggctg actgctcgcg ggcgcatggg cctgcgcggc cgggccgagg tcatcccggt
       61 cacgggatcg ttccggatgg aagccctgcg gagacgcacg tggtagttcc gtccggcacc
      121 catggccacc ccaagaccgg cgcgtccgtt ctgcaggttg tccccgggag acgcatgacg
      181 acgcagtcct ttcccacacc ccccgcaccc ggcatctccc ccctccaggg cctgaccgaa
      241 tcattgccga gcgacaaggc gcggacgcga atcctggtgg tggatgatag cccactggac
      301 ctccaggttc tggaggatct gctgacgagc gaggggtatc tggtgacttc cgcccaggat
      361 ggcgaagagg ccctggccaa ggtggccgtc gagcctccgg acctgatcct cctcgacgtc
      421 gtcatgccga agctggacgg ctacgaggtc tgccggcggc tgaaatccga cggacgaacg
      481 atcctcatcc cggtggtgat gatcacgtcc ctgcaagcga cccacgagcg gatcaaggga
      541 atcgaggccg gggcggacga gttcctgagc aagcccttca accgccaaga gctcatgaca
      601 cgggcacagt ccttgctcaa actcaaacgg cacacggatg agctggagag tgccgagacc
      661 gtgctcttca ccttggcgct cagcgtcgag gccaaggatc cttacacgga ggggcattgc
      721 gaccgtctgg cccgctttag cgtggccctc gggaggggat tggatttgcc ccctgaatat
      781 ctgaaggcct tgcaccgggg gggcatcctg catgacgtag ggaaaatcgg catcccggac
      841 gccatcctgt tgaagccggg acccatcacc gagacagagc gtgtcgtgat ccagacccat
      901 ccgagcattg gggagcgtat ctgcgctccc ctgaagtccc tgcgcctggt cctccccatc
      961 attcgccacc accacgaacg atgggacggg agcggctatc ccgacggcct ggccggggag
     1021 gcgatccccg tgaccgcgcg catcctgcaa gttgcggatc tgtacgacgc catcacgacc
     1081 cagcgtccct acaagccggc ctacagcccc gaacgcgcgt ttgagatcat gcgggaagag
     1141 acggccaagg gttggtggga cggtcgcctc attgagacat tcatcgccct catcacgcaa
     1201 acgcgcaaga tgcaatagac catggccttg ccttccgagg aggaggcatc cctggatggc
     1261 ccctccattc cagttcccgc cgcgcccagg tttagcgcga gggagtggcc gcgggaaccg
     1321 gctggaactc ctgggtcttg gtgagcttgc cgccagtgaa ccagtaactc accccatcgg
     1381 tgtaatacca ccacacctca gactcgccgc cgggtccgac ctgctcccgc ttctcgggga
     1441 ggccgaattt ctggaggatc ttcccgcgat ccgactccga tccatagaag gcaatctgcc
     1501 gctccacctc aggccaggcc ggagcgaacc gaagcacctg gttgacgtgg atttgactat
     1561 cgcgattcag gagcgtcgtg atgtcgagtg gctcaggacg ccggtacttc accccgtcga
     1621 accccgtctt gtcg
//
LOCUS       DBNGZC010000167         2211 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2263248, whole genome shotgun sequence.
ACCESSION   DBNGZC010000167 DBNGZC010000000
VERSION     DBNGZC010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2263248"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="AC16G0_03100"
     CDS             <1..680
                     /locus_tag="AC16G0_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010044112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098640.1"
                     /translation="LRGEDWDHTAVDPESRLLLCLVPGKRDGAACQEVIQQVHDRTGG
                     RTDLLITSDEHAPYETAIHEVYGLKLPQPKRPGPGRPPKPRTVMPPELCYATVRKRRE
                     KGRVVEVVRTVVFGTIALLHALLSRSTVSTTINTSFVERHNGTDRHQNARKHRKTYSF
                     SKEIELHHAASYFIGYSYNFCWCVRTIRIRGEDGCWQPRTPAMAASLTDHVWDLWEWL
                     TYPAKPR"
     gene            969..2141
                     /locus_tag="AC16G0_03105"
     CDS             969..2141
                     /locus_tag="AC16G0_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098641.1"
                     /translation="MTRPQYDDLWEDAERVRVLLAQWARSAPELFPADFDRGYHLHGF
                     GRESRKLPGVKLRKIVLADGTAYGLRPSFVASYMTGTVEELAYPLLLMEHGVPAWLLT
                     LGFGRSDMYWYRLLERLGHNSLVGTTVCDAARLPAHLTADEHHADWAGQKGYIPTTVG
                     GDCILGIALTAAADDDHLQEAYGVFAAEARNVDPEYAPETVNTDGWAATRNAFRAWFP
                     LITAVLCFLHGFLKIRDRGRKAHDLHHRVWDVYRAATAEEFRRLMTALRQWCAARTWT
                     AAVQEMLEKLWNKAGSYVAAYDHPGCHRTSNAVDRPMNRLCRLMYAGRGLHGHQGSSE
                     LRLRGWALLRNFRPYARRSNRPRTYDSPAHQLNGQRYHEHGLHNLMVSTSLMGCRS"
BASE COUNT          397 a          713 c          727 g          374 t
ORIGIN      
        1 ggctccgggg tgaggattgg gaccatacgg cggtcgatcc cgagagccgg ctcttgttgt
       61 gtctggtgcc gggcaagcgg gacggggccg cctgccagga ggtcatccag caggtccatg
      121 accgaacggg aggacggacg gatctgctga tcaccagcga cgagcatgcc ccgtatgaga
      181 ccgccatcca cgaggtctat ggcctgaagc tgccccagcc gaagcgcccg gggccgggcc
      241 gcccgcccaa gccccggacg gtgatgccgc cggagctgtg ctacgcgacc gtgcggaagc
      301 ggcgggagaa agggcgtgtg gtggaggtgg tgcgcacggt cgtcttcggg acgatcgcgc
      361 tgttgcacgc cctgttgagt cgctcgacgg tgagcacgac gatcaacacc tcgttcgtgg
      421 agcggcacaa tgggacggac cggcatcaga acgcccgcaa gcatcgcaag acctacagtt
      481 tctccaagga gattgaactc caccacgcgg cctcatactt catcgggtat agctacaact
      541 tttgttggtg tgtgcggacg atacgcatcc ggggtgagga tggctgctgg cagccccgga
      601 ctccggcgat ggccgccagc ttgacggatc acgtctggga cctctgggag tggctcacgt
      661 atccagccaa accgaggtag ctattctcag acaccacccg attcttcacc atccaataat
      721 tcgactcggg aggctgggca gtaacccggc aaccaagtaa atcatgtacc ataacaagtt
      781 ttctgatcct atcggattgc aggtgttgaa gtgccgggta tccggccggc ggccttcttg
      841 gcatccttct tcttaccagg gaagaggatg cgaggatgga cacgcgaccg aagacccggc
      901 aggtgcatcg tcaacgaagc gctccctcgc cgcgagtgcc gcgcggagcc aaacagatcg
      961 tcatcccgat gactcggccg cagtacgacg acctctggga ggatgccgaa cgcgtgcgcg
     1021 tgctgctcgc gcaatgggcg cgatcggctc ccgagttgtt cccggcggac ttcgaccggg
     1081 gctaccacct gcacggcttc ggacgcgagt cgcgcaagct ccccggcgtg aaactgcgga
     1141 agatcgtcct ggccgacggc accgcctacg gactccgccc cagcttcgtc gccagctaca
     1201 tgaccggaac ggtcgaggag ctggcgtatc cgttgttgtt gatggagcac ggcgtccctg
     1261 cctggctgct gacgctcggt ttcggccgga gtgacatgta ctggtatcgc ctcctcgagc
     1321 ggctggggca caactccctg gtggggacca cggtctgcga cgcggcccgg ttgcccgcgc
     1381 acctgaccgc cgacgagcat cacgccgact gggcgggcca gaaaggctac atcccgacca
     1441 cggtcggcgg cgactgcatc ctgggcatcg ccctgacggc cgcggccgat gacgatcatc
     1501 ttcaggaggc ctacggcgtg ttcgcggccg aggcgcggaa tgtcgatccc gagtacgctc
     1561 ccgagacggt caacaccgac ggctgggcgg cgacgcgcaa tgcgttccgg gcgtggttcc
     1621 ccctgatcac ggccgtgctg tgcttcctgc acgggttctt gaagatccgc gaccgcggcc
     1681 gcaaggccca cgacctgcac catcgggtgt gggacgtcta ccgggccgcc accgcggagg
     1741 agttccgacg gctgatgacc gcgcttcggc agtggtgtgc cgcgcggacc tggaccgccg
     1801 ccgtgcagga gatgctggag aagctgtgga acaaggcggg gtcctacgtg gccgcctatg
     1861 atcatccggg ctgccatcgc acgagcaacg cggtggaccg tcccatgaac cggctgtgcc
     1921 ggctgatgta cgcgggccgg ggcctgcacg gccatcaggg ctcgtcggag ttgcggctgc
     1981 ggggctgggc gctgctgcgg aacttccgcc cctatgctcg acggagcaac cgcccccgca
     2041 cctacgacag tcccgcccat cagctcaacg gccagcgcta ccacgagcat gggctccaca
     2101 acctcatggt gtccacctca ctgatgggct gcagaagctg agaacctgca atccggtaga
     2161 atcagtataa ttccaatatg agcccgcctt ccaatcgtaa gcagtctccg g
//
LOCUS       DBNGZC010000168         5619 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1676338, whole genome shotgun sequence.
ACCESSION   DBNGZC010000168 DBNGZC010000000
VERSION     DBNGZC010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1676338"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..681
                     /locus_tag="AC16G0_03110"
     CDS             163..681
                     /locus_tag="AC16G0_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098642.1"
                     /translation="MWRAMTHLLVAVAVLVAWGPSSRADTTPDEVAQEAEAAIRSAME
                     AWAYEAYWRLWEMGTRTSRSALPREDFTDRMRHGNTQPAAGKQVEMIQVISTRPKSVL
                     VYVRFGVEDKRRAWVDSTQRPFLLSFEDGGWRVSLWDFVGLANFFPPDYFPRPPVIIP
                     PPIAPRPKGMTR"
     gene            complement(804..1946)
                     /locus_tag="AC16G0_03115"
     CDS             complement(804..1946)
                     /locus_tag="AC16G0_03115"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5098643.1"
                     /translation="MRIFPIALMVLSHTVVDSTQNILPVFLPLLQDRFGLNYFQVGVA
                     AALLNVSSSMIQPAFGWMSDRWPTRWFIPAGILWTGMFIGLLGLVPNYWALLGVVVLT
                     GVGTAAFHPIASIAVAHASRAQRGLGMSFFSAGGNLGFALGPIIATWLLAWFDLRGSL
                     MMFVPCLLAASAVFAWRGEFESPAKVRVQSVAHGDAPSPWGKLAVLCVLVTLRSWGYS
                     GLIIFLPLLLHEQGAPLTTGAWALFVFLFFGALGGLLGGYLSDRFGRRQVIALSLLTY
                     PLLMTGALLFTGPVRWLVLATGGMALLASFAVTVVFAQELLPHSLGLVSGLMFGLSFG
                     TGGLGVGLSGFMADLLGLRESVWILIMLPGLAGLLALSLGPPRRDV"
     gene            complement(1950..2753)
                     /locus_tag="AC16G0_03120"
     CDS             complement(1950..2753)
                     /locus_tag="AC16G0_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007954706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HIR5098644.1"
                     /translation="MRGRPGLTSAGALDQAGSSAGGARPQTLRKPLLIRKTIVRGTIV
                     NVIAVLLGSGIGLAVGNRLPPRLQQIITTGLGLSTLLIGMQMALKVENMLVVIASMVI
                     GGMVGELLAIEARLERAAEWLKGWARSSSGSFVAGYVTASLVFCVGPMTIVGSIQEGI
                     SGNAQIIYTKSLLDGAASVAFASSLGIGVSFAAITVLVIQGTLTLLGAQLAFLLRPEM
                     LNELTATGGLLIVAIGLLLLEVKRLRVANLLPALLMAVLLTAGRLSLGW"
     gene            complement(2839..3108)
                     /locus_tag="AC16G0_03125"
     CDS             complement(2839..3108)
                     /locus_tag="AC16G0_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HIR5098645.1"
                     /translation="MALYIVSDECSSCGACETECPNQAISEGDSSYVINADLCTECVG
                     FFAEPQCTGSCPTDSIKPNPDAQESKDALLAKFKKLHPDKEPVLN"
     gene            complement(3361..4182)
                     /locus_tag="AC16G0_03130"
     CDS             complement(3361..4182)
                     /locus_tag="AC16G0_03130"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR5098646.1"
                     /translation="MELSDLSKISTIVTDLLVRYSFQALAAVAILAAGLLCARAAGNF
                     IERSLKKTPIEAHLQRLMVRGGKGLVILFTLILALDKFGVQVTALVAGLSVAGIAGGF
                     ALQGILGNLAAGVSIMLSRHFRIGDYIEIGNVKGQVQSIELTMTILRTLDDARVIVPN
                     RRIVGEIIYNYTGERRVPLSVEVSYQADLDKVVSVVREVLAANPRVLKEPSPEVGITR
                     LADSGIEISLRPWCKAEDFWRVQYEVYRAILDRFREREIEIPYPQREVRLLNAAP"
     gene            4402..5328
                     /locus_tag="AC16G0_03135"
     CDS             4402..5328
                     /locus_tag="AC16G0_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HIR5098647.1"
                     /translation="MREGSTTAGESWGVFPQKAEPPKSVLELAEQVQADGGSALAIYR
                     EPVGDAWQIFALLPLEQVHPTPYQRDLSPTHAKRLREIIKKLDRFIDPVVVVRHGLGE
                     YWTPNGNHRRDALQKLKAKHIPVILIPEPEVAFQILALNTEKAHNLKEKSLEVIRMFR
                     GLMEEGPRKAEEDFTFQFEEAHLITLGLLYEKYPRFSGSVHAPILRRVDKFLKLTLPK
                     AHEERQRRAGMVESADKLLADVVARLKRRGINHPYVKNFVMARCNPLTRARKTLPGFD
                     QAWEKVHAALEKFDVDSIRTEDIARAALGPTA"
     gene            complement(5426..>5619)
                     /locus_tag="AC16G0_03140"
     CDS             complement(5426..>5619)
                     /locus_tag="AC16G0_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HIR5098648.1"
                     /translation="YGMPILDVDKARTVMVIKRSMSPGFAGIDNELYYMDRTMMLFGD
                     AKVFVGELVKELTWSHVRV"
BASE COUNT         1131 a         1817 c         1768 g          903 t
ORIGIN      
        1 ccgccaccgt ccggaacatg atccggcctc aatcggccgt gcccggtttc ccggacaaga
       61 cccattcgca gtgagaccgt cctgcgctgc gccagcactc catgctatcc tgtacccact
      121 actcttcccc gattccagtt ctgacgagtg cggaggtccg gcgtgtggcg tgcgatgacg
      181 catttgctgg ttgcggtggc cgtcctggtg gcgtggggcc cttcatcgag ggcggacacg
      241 acgccggatg aagtggcgca ggaggcggag gcggcaatca ggtccgccat ggaagcctgg
      301 gcttacgaag cttactggcg actatgggag atgggaaccc gcacgagtcg atcggctctc
      361 ccccgagaag atttcacgga ccggatgcgg cacgggaaca cccagcctgc agcggggaaa
      421 caggttgaga tgattcaggt gatctccacc cgacccaagt cggtgctggt ctatgtcagg
      481 ttcggcgtgg aggacaagcg cagagcatgg gtggacagca ctcagcgtcc ctttctgctc
      541 agcttcgagg acggaggctg gagggtgagc ctgtgggact tcgtcggtct cgcgaacttc
      601 ttcccgcctg attacttccc taggccgcca gtgatcattc ctcccccgat tgcgcccaga
      661 ccgaagggga tgacgcgtta gtcaagggga gggtgagtgg cgaggcaggc ggtgacgcag
      721 agagtccgca gccagcagtt caccgccgtt gcgggctggc gtatttcggc ggggtggcga
      781 tccaggggta tggcgggccg aggctacacg tcccggcgcg ggggcccgag actgagggcc
      841 agcaggcccg cgagtccggg aagcatgatc aggatccaga cgctctcgcg caaacccaag
      901 aggtcggcca tgaaccctga cagcccaact cccaagccgc cggtgccgaa cgacaggccg
      961 aacatcagtc ccgacaccag cccgagggaa tggggcagca attcctgtgc gaagacgacg
     1021 gtgactgcga aggatgccag aagggccatg ccgcctgtgg ccagaaccag ccacctcacc
     1081 gggccggtga acagaagggc tcctgtcatg agcaacggat aggtcaggag cgagagcgca
     1141 atcacctgcc gccttccgaa ccgatccgac agatagccgc ccaggagacc gcccagggcg
     1201 ccaaagaaga gaaacacgaa cagcgcccag gccccagtcg tgagcggggc gccctgctcg
     1261 tggaggagca gcgggagaaa tatgatgagg ccggaatagc cccacgagcg gagcgtgacc
     1321 agcacacaca ggacggccag tttcccccac ggggaggggg catcgccgtg tgccacggac
     1381 tggacgcgca ccttggctgg agactcgaat tccccgcgcc aggcaaacac cgccgaggcc
     1441 gcaaggaggc acgggacgaa catcatcagg gaccctcgca ggtcgaacca ggccaacagc
     1501 catgtggcga tgatgggtcc cagggcgaat cccaggttcc cgccggcgga aaagaacgac
     1561 attcccaggc cgcgctgggc tcgtgaggca tgtgccacgg cgatggacgc gatcggatgg
     1621 aaggccgccg taccgacccc cgtgagcacc accaccccaa gcagggccca gtagttcggc
     1681 acgaggccca gcaaccctat gaacatccca gtccacagga tcccggccgg gatgaaccac
     1741 cgcgtgggcc agcgatcgga catccagccg aaggccggct ggatcatgga ggacgagaca
     1801 ttcagcaagg ctgcggccac cccgacctgg aagtagttga gaccgaaccg atcctgcaag
     1861 agcggcagga agaccggcag gatgttctgc gtgctgtcca cgaccgtgtg gctgaggacc
     1921 atgagtgcaa tgggaaatat tcgcaccgat caccacccca aggaaagacg tcccgcggtc
     1981 agcagcaccg ccatcagcaa cgcgggcagc aggttcgcga cgcggagacg cttcacctcc
     2041 aggagcagca gaccgatggc cacgatcagc aggcctccgg tcgccgtgag ttcgttcagc
     2101 atctcgggcc gcagcagaaa ggcgagctgc gcccccagga gcgtgagcgt gccctggatc
     2161 accaagaccg tgatggctgc aaagcttacc ccgatcccca agctggaagc gaaagcgacc
     2221 gacgccgccc cgtccaggag ggacttcgtg tagatgatct gtgcgttgcc gctgatgcct
     2281 tcctggatcg accccacgat ggtcatgggt cccacacaga acaccaagct ggcggtgacg
     2341 tacccggcca cgaagctgcc ggagctcgat cgagcccagc ccttcagcca ctccgccgcc
     2401 cgttccaggc gcgcctcgat ggcaagcaac tcccccacca tcccccctat caccatgctg
     2461 gcgatcacga ccaacatgtt ctcgaccttc agcgccatct gcatgccgat gaggagcgtg
     2521 gacaggccca gacccgtggt gatgatctgc tggaggcgcg gcggcaggcg attccccacg
     2581 gccaggccga tcccgcttcc cagtagcacg gcgatcacgt tcacgatggt tccgcgcaca
     2641 atcgtctttc ggatgaggag cggtttgcgt agcgtctgcg gccgggcgcc gcccgcagat
     2701 gaaccggcct gatcgagcgc cccggcactc gtgagcccgg gacgtccccg catgccgtgg
     2761 gggggctgga aaaaagcaac ggggccacga atgcctcggc atcttggccc cgtcaagatg
     2821 aaacgcttct tccgggaact agttcagcac cggctccttg tccgggtgca gcttcttgaa
     2881 cttggccagc agcgcgtcct tgctctcctg cgcgtccgga ttcggcttga tgctgtccgt
     2941 cgggcaagag cccgtgcact ggggctcggc gaagaagccg acgcactcgg tgcacaggtc
     3001 ggcattgatc acgtacgagg agtctccctc actgatggcc tggttcgggc actcggtttc
     3061 gcaggcaccg caactgctgc actcatcgct gacgatgtaa agggccattg atctcctccg
     3121 gctttgcgcg atttgccgcg cagggttctt agggaatgtg caacgctggc atgactatag
     3181 gcgtcgagcc tgacgcggca aagagaaaaa aggggcatcc agtgaaattt cgtacaatgg
     3241 cgcgtttttc cagacaatcg atgcaatcgt gtctctggcg cgtgcgagcc agcacgggga
     3301 gccacaccgg ttgccggcat ttgccgaagg gcttcacgcc caccccgccc ggggcaccgc
     3361 ctagggagct gcattcagca ggcgcacctc gcgctgggga taggggatct cgatctcccg
     3421 ctcgcggaag cggtcgagaa tcgcccgata cacttcgtac tgcacgcgcc agaagtcctc
     3481 ggccttgcac cacggccgga gcgagatctc gatccccgag tcggccagcc gggtgatccc
     3541 cacctcggga gatggctcct tcaggacgcg gggattcgcc gccaggacct cccgcacgac
     3601 gctcaccacc ttgtccagat ccgcctggta gctcacctcc acggagaggg gcactcgccg
     3661 ttcaccggtg tagttgtaga tgatctcccc cacgatccgg cgattcggca cgatgacccg
     3721 cgcgtcatcg agcgtgcgca ggatcgtcat ggtcagttcg atggactgga cctggccttt
     3781 gacgttcccg atctcgatgt agtcgccgat tcgaaaatgc cgggagagca tgatgctgac
     3841 gcccgcagcc aggttcccca ggatgccctg gagggcgaag ccgccggcga tcccggccac
     3901 gctgagtccg gcgaccaatg ccgtgacctg gaccccgaat ttgtccaagg ccaggatcag
     3961 cgtgaacagg atgaccaggc ctttcccccc acgcaccatc aggcgttgga ggtgggcctc
     4021 gatgggcgtc ttcttgagcg accgctcgat gaaattcccg gccgcgcgcg cacacaggag
     4081 accggcggcc aggatggcca cggccgccag cgcctggaag ctgtaccgca ccagcaagtc
     4141 tgtcacgatg gtggagatct tggagagatc ggagagttcc atgggcgcct cctctgcttg
     4201 ggatctgaac agccgccaca tcgttgctgc caggatatcc gaggcctcgc ggcagggtca
     4261 agtcatccgc tcgatggcgt cgcgggcgca ggggggaaat gcgccgatgc acccgcgcta
     4321 caggccacac cgaaaatcac ttgtcgcctg tccgctgctc tgctatggaa gaggaaccaa
     4381 gaagccggaa aggaggaaaa aatgcgcgag ggatcgacta cggccggaga atcatggggg
     4441 gtttttccgc agaaggctga gccgccgaaa tcggtcttgg agctggcgga gcaagtgcag
     4501 gcggacggcg gttccgccct cgcgatctat cgagagccgg tgggcgacgc ctggcaaatc
     4561 ttcgccctgc tgcccctgga acaggtgcac cccacgccct accagcggga tctgtcaccc
     4621 acccacgcca agcggttgcg tgagatcatc aagaagctcg accggttcat cgacccggtg
     4681 gtggtcgtcc gccacgggct cggggagtac tggaccccca acggcaatca ccggcgggac
     4741 gccctccaga agctgaaggc caagcacatc cccgtcattc tcattccgga gccggaagtt
     4801 gctttccaga tcttggcgct caacacggaa aaggcccaca acctgaagga aaagtctctg
     4861 gaggtcatcc ggatgtttcg tggcctcatg gaggagggac ctcgtaaagc ggaagaggat
     4921 ttcaccttcc agttcgagga ggctcacctc atcacccttg gactcctgta cgagaagtat
     4981 ccccgcttct cggggagcgt acatgccccc atactgcggc gagtggacaa gttcttgaag
     5041 ctgaccctcc ccaaggcgca cgaagagcgc cagcgccgcg ccgggatggt cgagagcgcc
     5101 gacaagcttc tggcggacgt cgtcgcccgt ctaaagcggc gcggcatcaa tcatccctac
     5161 gtcaagaatt ttgtcatggc ccgctgcaac cccctgaccc gtgcccggaa gaccctgccc
     5221 ggcttcgacc aggcctggga gaaggtccac gccgctctgg agaaattcga cgtggacagc
     5281 atccggaccg aggacattgc ccgcgccgcc cttggtccga ccgcctgacg accatcccaa
     5341 gagccggaat cagacagtcc cgatttgccc gctcgcggaa ggccactgac atacgtctga
     5401 cgccgaaccc cgaggtgtcc gccgatcaca cccggacgtg ggaccaggtc agctccttta
     5461 ccagttcccc cacgaagacc ttggcgtcgc caaagagcat catggtcctg tccatgtagt
     5521 agagctcgtt gtcgatgccg gcgaagcccg gactcatgct gcgcttgatc accatgaccg
     5581 tccgagcctt gtccacgtcc aggatgggca taccgtaga
//
LOCUS       DBNGZC010000169         3353 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2654473, whole genome shotgun sequence.
ACCESSION   DBNGZC010000169 DBNGZC010000000
VERSION     DBNGZC010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2654473"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="AC16G0_03145"
     CDS             complement(<1..121)
                     /locus_tag="AC16G0_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIR5098649.1"
                     /translation="MRALDGVRVLDMSRFIAGPFCCQILGDNGADVVKVEKPDG"
     gene            complement(256..1230)
                     /locus_tag="AC16G0_03150"
     CDS             complement(256..1230)
                     /locus_tag="AC16G0_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR5098650.1"
                     /translation="MTITRATSLKPYTFENIVVGEEFGPVVGAVSDLKVKTHAFAVDD
                     YGSWYFHGSPFGARIGHPTLLATDILMLFTLVYDMTPPCEAGLHARNELEFLKPIFLG
                     QKVTITGKTTEKYQKRAQNYRVLEGMVLDQAGAACLRMRATETVGLNPSTTVGKGMST
                     PPPDAITGEVPGGAPSVVQASRWVPVGAALPALSKYTTLEQSTAYSGYPHGWADGGSR
                     AMWYNQHTDPEDARRHGHTDAVVQGLCSAAYISEMCTNFFGPSWLTSGRLSTAFIRPV
                     IVRDTITAGGLVKRLEEEGGKRRLWLDVWCKNQRGELATVGRASALVD"
     gene            complement(1327..2265)
                     /locus_tag="AC16G0_03155"
     CDS             complement(1327..2265)
                     /locus_tag="AC16G0_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR5098651.1"
                     /translation="MTAPRQGVVARYENILLEEDFGPIHEMVDDYKIKKFAFMMDDYT
                     PWYFGESPFGKRIGHAAILANDLLQLFTQVYDPQTVVGLHTQEELWFVNPVLAGERAR
                     LYGKYVEKYERRGKGYAVMEADARGEDGRVLLRHRGIEILHIQPGPVVGKGTAEPPEK
                     RVTGVYRKDAEPVDRADRGLVPGTPLVPLVKTVMQDQIAVFSLVGKHLRNIHTDIQVA
                     RKAGLKDTLAQGMMESNYITELLTHFFGPSWFTTGWQKVKFLRPVFAGETLTIRGVIA
                     GEQPEAGGTRVDLEVWVENAAGDMTAAGWASGRLRG"
     gene            complement(2282..3061)
                     /locus_tag="AC16G0_03160"
     CDS             complement(2282..3061)
                     /locus_tag="AC16G0_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5098652.1"
                     /translation="MPLLELRGISKRFDAVQALRDVDLAVDLGEVLALVGDNGAGKST
                     LIKIISGVHPPDEGEVWLDGRRIRVDSPETAKTLGIETVYQDLALFEHANVSANLFAG
                     RELTRRLLGILPILDESRMRSFAQDALARLRIQIPGVDRSMNLLSGGQRQCVAIGRAI
                     AFGTKLVIMDEPTAALGAVEAAKVLGLVRTLRERGITVMVVSHNLQHVFSIADRIAVL
                     RHGSMVGTVRPAETTGEAVVRMIVGADDAAVDIPGSGPAMQ"
     gene            complement(3064..>3353)
                     /locus_tag="AC16G0_03165"
     CDS             complement(3064..>3353)
                     /locus_tag="AC16G0_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR5098653.1"
                     /translation="AAVYCHNDEMALGAMQALEARGRAKGVIVTGMDGLQSTMKDIND
                     GKVTGTVYYHHMFPEALDLAMKVLKGQPVPKRTMLETPFVSQENVAQFLGK"
BASE COUNT          555 a         1172 c          995 g          631 t
ORIGIN      
        1 cgccgtccgg cttctcgacc ttgaccacgt cggccccgtt gtcgcccaag atttggcaac
       61 aaaaggggcc ggcgatgaat cgagacatgt ccaggacgcg gacgccgtcc agtgccctca
      121 tcccttccgt ctcctccgga gaattccaat cggctgatcg tccatgggga tctgcctcgg
      181 gtcacggcca cgcatttcgc agaagactct ccagcgcacg cggaccgtct tctcctcccg
      241 gcacggatcc ctgccctagt ccaccaaggc gctggcgcgc ccgaccgtcg ccagctcccc
      301 tcgctgattc ttgcaccaga cgtccagcca caatcgccgc ttccctccct cctcctccag
      361 gcgcttcaca agaccgccgg ccgtgatcgt gtcccggacg atgacgggtc ggatgaacgc
      421 ggtggagagc ctcccgctgg tgagccagga gggcccgaag aagttcgtgc acatttcgga
      481 aatgtacgcc gcagagcaca gcccctgcac cacggcatcc gtgtggccgt gacgccgggc
      541 atcctcgggg tcggtgtgct ggttgtacca catggctcgg gagcccccgt cggcccagcc
      601 atgcggatat cccgagtagg cggtgctttg ctccaacgtc gtatacttgc tcaacgcggg
      661 gagcgccgcc ccaactggca cccagcgact cgcctgaacg acgctcgggg cgccgcccgg
      721 cacctcgccc gtgatcgcat ccggcggagg ggtgctcatc cccttcccga cggtcgtgct
      781 gggattcagg cccaccgtct cggttgcccg catgcggaga caggctgcgc cggcttggtc
      841 caggaccatc ccttccagca cccgataatt ctgggcgcgc ttctggtact tctccgtagt
      901 cttgcctgtg atggtgacct tctgtccgag gaagatgggc ttcaggaact ccagctcgtt
      961 gcgcgcgtgg agcccggcct cgcatggcgg cgtcatgtca tagaccaggg tgaacagcat
     1021 gaggatgtcg gtggcgagga gcgtggggtg gccgatgcgc gccccgaagg gcgagccgtg
     1081 gaagtaccag gagccataat catccaccgc aaaggcgtgc gtcttgactt tcaaatcgga
     1141 gaccgcccca acaaccggtc cgaactcctc gccgacgacg atattctcaa acgtgtacgg
     1201 cttgagcgag gtggcgcgcg tgatggtcat gatcgttctc cttactccgg ttctggcctc
     1261 atccgggcag gcaggcttcc cctcggaagc caggcacgca agaccgggac gcgtttctcc
     1321 cggcgatcat cctctcagcc ggccgctggc ccagccggcc gcggtcatat ccccggccgc
     1381 attctccacc cagacctcca ggtccacgcg agtcccgccc gcctcgggct gctcgccggc
     1441 gatcacgccg cggatggtga gtgtctcgcc cgcaaagacg ggccggagga atttcacctt
     1501 ctgccaccct gtcgtgaacc aggagggtcc gaagaagtgc gtgagcagct cggtgatgta
     1561 attggattcc atcatcccct gggccagggt atccttgaga cccgccttcc gggcgacctg
     1621 aatgtcggtg tggatgtttc ggaggtgctt tccgaccaag gagaagaccg cgatctgatc
     1681 ctgcatgacg gtcttcacca gggggaccag cggcgtgccg ggaaccaggc ccctatcggc
     1741 ccggtcgacc ggctccgcgt ccttccggta gacccccgtc acccgcttct ccggcggctc
     1801 ggcggtgccc ttgcccacca ccgggcctgg ctggatgtgc aggatctcga tcccgcgatg
     1861 acggagcagc acgcggccgt cctcgccccg cgcatccgcc tccatgaccg catacccctt
     1921 gccgcgccgc tcgtactttt ccacatactt gccgtagagg cgggcccgct ccccagctaa
     1981 gaccggattc acgaaccaca gctcttcttg cgtgtgcagc ccaaccaccg tctgtgggtc
     2041 gtacacctgt gtgaaaagct ggagcaggtc attggccaga atggcggcat ggccgattcg
     2101 ctttccaaac ggtgactcgc caaagtacca gggagtgtaa tcgtccatca tgaaggcgaa
     2161 tttcttgatc ttgtaatcgt cgaccatctc gtggattggt ccgaagtcct cctcgagcag
     2221 gatgttttcg tacctggcca ccacgccttg tctcggtgct gtcaccatgc tctcctcgct
     2281 ctcattgcat ggccgggccg ctgcctggga tatccacggc ggcatcgtcc gcacccacga
     2341 tcatccgcac gaccgcctcg cccgtcgtct cggccggccg gaccgtgccc accatgctgc
     2401 catggcggag gacggcgatc cggtccgcga tcgaaaacac atgctgcagg ttatggctca
     2461 cgaccatcac cgtgatcccc cgctcgcgta gggtccggac gagacccagc accttcgccg
     2521 cctcgactgc ccccagggcc gccgtgggct cgtccatgat gacgagcttc gttccgaagg
     2581 cgatggcgcg cccgatggcc acgcactgcc gctggcctcc ggacaagagg ttcatggacc
     2641 gatccactcc ggggatctgg atgcgcaggc gggccagggc atcctgggcg aagctccgca
     2701 tgcgactctc atccaggatc gggagtatcc cgagaaggcg ccgggtcaac tcccgacccg
     2761 cgaacaggtt ggcgctgaca ttcgcgtgct cgaacagggc caggtcctgg tacacggtct
     2821 cgatgcccag cgtcttggct gtctccggcg aatccacgcg aatgcgacgt ccgtccagcc
     2881 agacttcgcc ctcatcgggc gggtgcacgc ccgagatgat cttgatcagc gtggacttcc
     2941 ccgccccgtt gtctccaacc agagccagga cctcgcccag gtccacggcg aggtccacgt
     3001 cccgcaacgc ctggacggcg tcgaatcgtt tgctgattcc tcgcaactcg aggagcggca
     3061 tgactatttc cccaggaact gtgccacatt ctcctggctg acgaagggtg tctccagcat
     3121 cgttcgcttg ggtaccggct ggcccttcag caccttcatc gccaggtcca gcgcctcggg
     3181 gaacatgtga tggtagtaca cggtcccggt caccttcccg tcgttgatgt ccttcatggt
     3241 ggattggagc ccgtccatcc cggtgacgat gacccccttc gcccgacccc gagcctccaa
     3301 ggcctgcatg gcgcccaagg ccatctcgtc gttgtggcag tagaccgcag cga
//
LOCUS       DBNGZC010000170         2966 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2361438, whole genome shotgun sequence.
ACCESSION   DBNGZC010000170 DBNGZC010000000
VERSION     DBNGZC010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2361438"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..601)
                     /locus_tag="AC16G0_03170"
     CDS             complement(<1..601)
                     /locus_tag="AC16G0_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR5098654.1"
                     /translation="MLVCRIHAAEDLRMESMPVPEPRPDEVCLKLGAGGICGSDLHYF
                     LHGRIGSFLIREPLIPGHEISGTAVKVGSGVTRVKEGDKVAINPSHACGMCKACRAGR
                     ENLCDSMRFLGSASVFPHVQGMFREYFVMAERQCHPIASAVTLSELAFAEPLSVALHA
                     VNRAGGLLGKSVLVTGAGPIGCLIVMAARLAGAAQVVVSD"
     gene            complement(637..792)
                     /locus_tag="AC16G0_03175"
     CDS             complement(637..792)
                     /locus_tag="AC16G0_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098655.1"
                     /translation="MARPCFAVCKTIEPASCEMTPDIVGVFLGLVLAARETRLRLSLP
                     ALFQRAG"
     gene            complement(805..1731)
                     /locus_tag="AC16G0_03180"
     CDS             complement(805..1731)
                     /locus_tag="AC16G0_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5098656.1"
                     /translation="MRAGQPALPPRDQRWRTAKAKPGASQRRAVSSGRLGGFRLAPVV
                     GCGFLALYLLGSLFPIYWIIVSSLKTRMDTLVLPPVWFFRPTLGAYMKVFVFEYYYQY
                     FFNTTIVAIATTMVSLALGSMAAYVIDRYPFRFSHLIAYTLLGTRMIFPIVYAIPLFL
                     LFRDLSLLDTLTGLVIAYTTFSLPYAVWIMSGFFSAVPTEIDDAAMVDGCSRFGAFCR
                     VILPLTAPGLAAAAIFILLLSWNEFLFALVLAGGGQAKTLPVAAAQLIGHREIQWNEL
                     CAVSTATIIPLLAFFALVHKHLVKGMIAGAVK"
     gene            complement(1728..2597)
                     /locus_tag="AC16G0_03185"
     CDS             complement(1728..2597)
                     /locus_tag="AC16G0_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5098657.1"
                     /translation="MKDRRTAVLMIAPTVLVVGLVFVYPLILSLWTSLHYQVLSQPHL
                     TRFIGLGNYQWLMREPRFWNALVRSLVFLIFTVAISMGAGTLVALLIDRYLGRWSWAR
                     ALFLMPMVITPVVVGIQWRFLLNDQFGVASWFFDAIRLPIGGTWLSDPIGAMFWVIAV
                     DLWYYTPYVILLAGAGLASIPTEVRDAVKVDGATPWQTVRYVLLPMLKPVLVVALLIR
                     TIGGLRAFDTIMIITDGGPGRATEVANILAYRYGLQYFDMGKASAIGWVMMLVALVLT
                     VVYIRLISLGRKA"
     gene            complement(2650..>2966)
                     /locus_tag="AC16G0_03190"
     CDS             complement(2650..>2966)
                     /locus_tag="AC16G0_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098658.1"
                     /translation="NQNKLLTVANTPPTRSSALNAPGNEQKYVWLSPLKEALTNSFDF
                     PKIPEWAEIMERVGAELHGAWSGQNTLPKALDRSNQLVTALMKERGYPVGTHKGAKLP
                     WE"
BASE COUNT          610 a          949 c          950 g          457 t
ORIGIN      
        1 cgtccgacac gaccacctgt gcggcgccgg cgagtcgagc cgccatgacg atcaggcagc
       61 cgatggggcc ggcccccgtc accagcacgc ttttccccag caggccaccc gctcggttca
      121 ccgcatgcag agcgaccgag agaggctctg caaaggccag ctcgctcagc gtgaccgccg
      181 aggcgatcgg atggcattgc cgctcggcca tcacgaagta ctcccggaac attccctgca
      241 cgtggggaaa gacgctggcg cttcccagga agcgcatgct gtcgcacagg ttctcccgcc
      301 ccgcccggca ggccttgcac atgccgcagg cgtgcgaggg attgattgcc accttgtccc
      361 cctccttgac gcgcgtcacc ccggatccca ccttgactgc cgttccagaa atctcgtggc
      421 ccgggatcag gggctcgcgg atgaggaaac tgccgatgcg accatgcagg aagtaatgca
      481 gatccgagcc gcagattccc ccggctccca gcttcaggca cacctcgtcc ggccgcggct
      541 ccggcaccgg cattgactcc atccgcaagt cttcggcagc gtggatccgg cataccagca
      601 tcgtggcctc ctgggtgaac ccctcccgtc cgagatctac ccggcgcgct ggaacagggc
      661 gggcagggag agcctgagcc gagtttcccg cgccgccagc acgagcccga ggaaaacccc
      721 gacgatgtcc ggggtcatct cgcacgacgc tggctcaatc gtcttgcaga cggcgaagca
      781 gggccgcgcc acgagcggct gaacctactt cacggctccg gcgatcatgc ccttcacaag
      841 atgcttgtga accaaggcaa agaaggcgag cagcggaatg atcgtggcgg tcgacacggc
      901 gcacagctcg ttccactgaa tctcgcggtg gccgatcagc tgtgcggccg ccaccggcag
      961 cgtcttcgcc tggccgccgc ccgccagcac gagggcgaag aggaattcgt tccacgagag
     1021 gagcaagatg aagatggccg cggcggccaa cccgggggcg gtcagcggca ggatcacccg
     1081 gcaaaaggcc ccgaaacggg agcagccgtc caccatggcc gcgtcatcga tttctgtcgg
     1141 gaccgccgag aagaagccgg acatgatcca caccgcgtac gggaggctga aggtggtgta
     1201 ggcgatgacc agacccgtca gcgtgtcgag caggctcaga tcacggaaga ggaggaacag
     1261 cgggatggcg tacacgatgg ggaagatcat ccgtgttccc agcagggtgt aggcaatgag
     1321 atggctgaag cggaacgggt accggtctat cacgtacgct gccatggacc ccagggccag
     1381 cgagaccatc gtggttgcga tcgccacgat tgtcgtgttg aagaagtact gatagtagta
     1441 ctcgaatacg aagaccttca tgtaggcgcc gagggtcgga cggaagaacc agactggggg
     1501 aaggacgagc gtatccatcc gcgtcttgag cgatgagacg atgatccagt agatggggaa
     1561 gagcgacccc aagaggtaca gggccaggaa tccacacccg acgaccgggg cgaggcggaa
     1621 ccccccgagc ctcccggagg atacggctcg acgctgggat gcccccggct tggctttggc
     1681 cgtccgccag cgttgatccc ttggagggag cgctggctgg ccggccctca tgcctttctc
     1741 cccaggctga tcaagcggat atataccacg gtcaggacca gggccaccag catcatcacc
     1801 catccgatcg cggacgcctt gcccatgtcg aagtactgga gaccgtatcg gtaggccaag
     1861 atgttggcca cttccgtggc gcggccggga ccgccgtccg tgatgatcat gatcgtgtcg
     1921 aacgcccgca agccgccgat ggtgcggatc agcaacgcca ccacgagcac cggcttgagc
     1981 atcggcaaga gcacatatcg gacggtctgc cagggggtcg ccccgtccac cttcaccgcg
     2041 tcgcgcacct ccgtgggaat ggaggcaagc ccggcgccag ccaggaggat cacgtagggg
     2101 gtgtagtacc agagatccac cgcgatcacc cagaacatcg cgccgatcgg atcgctgagc
     2161 caggtcccgc cgatcggaag tcggatggca tcgaagaacc aggaggcgac cccgaattga
     2221 tcgttcagga gaaaccgcca ctggattccg accacgaccg gggtgatgac catcggcatg
     2281 aggaataagg cccgggccca actccagcgc cccaggtacc gatcgatgag cagggcgacg
     2341 agggtccccg cgcccataga aatggcgacg gtgaatatga gaaacacgag ggatcggacc
     2401 agggcgttcc agaaccgcgg ctcccgcata agccactggt aattccccag tccgatgaac
     2461 cgggtgagat ggggttgcga caggacttga taatgcaggc tcgtccagag cgaaaggatc
     2521 aggggataca cgaagaccag accgaccacc agcacggtcg gggcgatcat cagcacagca
     2581 gttcgacggt ctttcacgga aaatatcgag ccacccgggg ggcagcacgg ctgccccccg
     2641 gttctcccct tattcccagg ggagcttggc tcccttgtgc gtgccgacgg ggtagccgcg
     2701 ctccttcatc agcgctgtca caagctggtt ggagcgatcc agcgccttgg gaagcgtgtt
     2761 ctgccccgac caggcgccat gcaactccgc cccgacccgc tccatgatct ccgcccactc
     2821 gggaatcttg gggaaatcga agctattggt cagcgcctct ttcagcggag acagccagac
     2881 atacttctgc tcgttcccgg gggcgttgag ggccgaggac cgggtcggcg gggtgttcgc
     2941 gacggtgagc agcttgttct gattct
//
LOCUS       DBNGZC010000171         2422 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1578262, whole genome shotgun sequence.
ACCESSION   DBNGZC010000171 DBNGZC010000000
VERSION     DBNGZC010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1578262"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="AC16G0_03195"
     CDS             complement(<1..714)
                     /locus_tag="AC16G0_03195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5098659.1"
                     /translation="MRIGLFTNNYRPLANGLATSVETFAQAFRRAGHAVTIVAPRYGQ
                     APEAEPDVLRVPGWRAPPHHAYVLPMSGWPGTAQAVAALDLDVFHAQHPFLLGAAAGR
                     WARRLKRPLVFTYHTRYDLYAHYVPGPSGLVARLAVRRALAFADRADLVIAPAAALAR
                     DLRARGLRTPVEVIPTGVALPPPLAADERHALRRGLGLDSGCPVCLSVGRLAPEKNQA
                     FLLSAFALILKDLPAARLVF"
     gene            complement(801..>2422)
                     /locus_tag="AC16G0_03200"
     CDS             complement(801..>2422)
                     /locus_tag="AC16G0_03200"
                     /inference="COORDINATES: protein motif:HMM:NF025017.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5098660.1"
                     /translation="PEDHGRAIFLVQRLYRKVREGVIRRGTDRLLEVAPFLTTVLLVS
                     VYPLLRYLRRDPFPAEIFRFAFISFWIGFLVVNMLRAHKTRQTILENLETDWLGRLRS
                     LGASLSHYAIFMPLKHEGNRHVLFQTFLSVERLRYPKSLVSLIPIVEAEDRITLEKLE
                     EVVSIFKDRIQVEPFVYPAEGLQVKCKDTSVSMAGRWLSQRVAEGTFSPGPGGVKVLI
                     IDADTILHPQDLAFRDLTHREEESRAVARGLRGVVLQSLTTYTANYWKVPMLPRLHNT
                     GFVLYQMGKMQSKGDYLVLGPGTSMDLRAFHEVDYFEPNRHNEDMQFRYKVVMEGYRV
                     APLKIPTWGQAPLTTKESWGQIARWARGAVDVKFVVRYQRKFSGAGSSLFRSKTFQAV
                     RALLANAMPPLTVLLPAQLILISWISPCVKDLWPVQVFLSPDVLALKLAGRSLCVSPM
                     AAFNLVFQTIVLTSSTLLGMVVVPHTLKPIIYQRWPRRWRRARNFLEWMRLVFTPINL
                     HNYFLMATAQLYMQGKLALGLPIAQTPVTRK"
BASE COUNT          465 a          736 c          842 g          379 t
ORIGIN      
        1 aaagaccagg cgagccgctg gcagatcctt caggatcaag gcgaacgcgg agagaaggaa
       61 tgcctggttc ttctctggag ccaggcgtcc cacggagagg cagacagggc atccggagtc
      121 gagaccgagg ccccggcgca acgcgtggcg ttcgtccgca gccaagggcg gcgggagggc
      181 cacgcccgtc gggatcacct cgaccggtgt gcgcagacca cgagcccgaa ggtcgcgggc
      241 cagagcggct gcgggcgcga tcaccagatc cgcacgatcg gcgaaggcca gggcccgccg
      301 aaccgcgagt cgggcgacca ggcctgacgg gccgggtaca tagtgggcgt agaggtcgta
      361 gcgggtgtgg taggtgaata ccaggggtcg tttgaggcgc cgcgcccagc ggcctgccgc
      421 ggcacccagg aggaacgggt gctgggcgtg aaacacatcg agatccagcg ctgccaccgc
      481 ctgggccgtg cctggccagc cggacatggg gagaacgtag gcgtggtggg ggggggcccg
      541 ccacccaggc acacgcagaa cgtccggctc ggcctctggg gcctgtccat agcgaggcgc
      601 cactatggtg accgcgtggc cggcgcggcg gaaggcctgg gcgaatgtct ccaccgaggt
      661 ggccaggccg ttggccaggg gccggtaatt gttggtgaag aggccgatgc gcatccgact
      721 aggccggcgt cacggggctt cccccggatg tctgcgctga gtgtccgtcg ccgaggcgag
      781 agccgggccc gcgcgaccgg tcattttcgg gtcacaggag tctgcgcgat gggcaggccg
      841 agggccagct tcccttgcat gtatagctgg gccgtggcca tcaggaagta gttatgcagg
      901 ttgatggggg tgaacaccaa tcgcatccac tccaggaaat tgcgggcccg gcgccagcgc
      961 cgcggccagc gctggtagat aatgggcttc agtgtgtggg ggaccaccac catgcccagg
     1021 agcgtggacg acgtgaggac gatggtttgg aaaaccaggt tgaaggccgc catgggactg
     1081 acgcacagac tcctgcccgc caacttgagg gccaggacgt ccggcgacag gaagacctgc
     1141 acgggccaga gatccttcac gcacgggctg atccacgaga tcaaaatgag ctgggctggc
     1201 aagaggacgg tgagtggggg catggcgttg gcgagaagcg cgcggacggc ctggaacgtc
     1261 ttgctccgga agagggacga tcccgccccg gagaacttgc gctggtagcg cacgacgaac
     1321 ttcacgtcca cggccccacg cgcccagcgg gcaatctgcc cccaggattc cttcgtggtg
     1381 agcggagcct gcccccaggt cggaatcttg agcggcgcga cccgataccc ctccatcacc
     1441 accttgtagc ggaactgcat atcctcgttg tgccggttcg gctcgaagta atccacctca
     1501 tgaaaggccc gaaggtccat gctcgtcccc ggtccgagga ccaggtagtc ccccttggac
     1561 tgcatcttcc ccatctggta caggacgaag cccgtgttgt gcagcctggg aagcatgggg
     1621 accttccagt agttcgccgt gtaggtcgtc agcgactgca gcacgacccc tcgcaggccc
     1681 cgggcgactg cccggctctc ctcctcgcga tgcgtcaggt cccggaaggc caggtcctgg
     1741 ggatgcagga tggtgtcggc gtcgatgatg aggaccttga caccacccgg cccgggcgag
     1801 aaggttccct ccgcgacacg ctgggacagc cagcgaccgg ccatgctgac ggaagtgtcc
     1861 ttgcatttca cctgcagacc ctcggcggga tagacgaagg gctcgacctg gatccgatcc
     1921 ttgaagatcg acacgacctc ttccagtttt tccagggtga tccgatcctc ggcctcgacg
     1981 ataggtatca gactcaccag ggacttggga tagcgcaggc gttccacgga gaggaaggtc
     2041 tggaacagga catggcggtt gccctcgtgc ttcaacggca tgaagatggc gtaatggctg
     2101 agcgacgccc ccagggaacg cagcctgccc agccaatcgg tctccagatt ctcgaggatg
     2161 gtctgccggg tcttgtgcgc ccgcagcatg ttcacgacca ggaagccaat ccagaatgag
     2221 atgaaggcga agcggaaaat ctcggcgggg aacgggtccc gccggagata gcgcagcagg
     2281 gggtagacgg aaacgagcag gactgttgtc aggaaggggg cgacctccaa cagacggtcg
     2341 gttcctcgtc ggatgactcc ttctcgtact ttccggtaca agcgctgaac caaaaaaatg
     2401 gctcgcccgt ggtcctccgg ct
//
LOCUS       DBNGZC010000172         2909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1873081, whole genome shotgun sequence.
ACCESSION   DBNGZC010000172 DBNGZC010000000
VERSION     DBNGZC010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1873081"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="AC16G0_03205"
     CDS             <1..953
                     /locus_tag="AC16G0_03205"
                     /inference="COORDINATES: protein motif:HMM:NF016292.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098661.1"
                     /translation="IALAIEGLLGGGVEAQPARMVKIGALTESWGPTPAIIGLRDGLT
                     ELGYRENEDFVIGVRFTQGDPAKLSEAARDLVRHGVDIIVTAEGGNTAKAAQMATSRI
                     PIVFMGGSDPVGVGLVQSLAHPGGNITGTADLDVELGPKRMEIFREMIPSLKRLLMPY
                     DATNTYAVAQLAVYRDAARRLTLTLVERPLRSEEEARAAITAVRKGEVDGIFSPRFVS
                     LNIPGFILETAAKRAIPTMFHGEFLVERGGLVSYSASDEQLGRQAARLVDKILKGAKP
                     GDLPVEQPTKFKLVINLKTAKALGLKIPQSVLIRADKVIQ"
     gene            950..1966
                     /locus_tag="AC16G0_03210"
     CDS             950..1966
                     /locus_tag="AC16G0_03210"
                     /inference="COORDINATES: protein motif:HMM:NF016292.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098662.1"
                     /translation="MRQFRIADVGLRIGPTLALVLGLALAVAGAPSLVDGQQVEGVSR
                     VGIVLHGGPYFAALEGLREGLKALGLEEGKRVTLAVRDAKGDLKAAEAAAQALERERV
                     SLIVSFATSVSLAVKRGTQQVPIIFVAGTDPVFAGLVEGIANPGGRLTGVHFLSSELT
                     AKRLEILKELLPRLRRVVTFYNPNNLGAQMSERTAREAGQTLRVEVVGRQVTSVEALR
                     AAVRGLKAEPADAYFFLADAMVISQAQAIIDAANAIRLPTMAQDPELAAKGALASYGI
                     SYRELGRETAAYVQRILAGTSPRDLPVQAVDRLGLAVNFKTAKALGVTIPQSILVRVD
                     QAIR"
     gene            2058..>2909
                     /locus_tag="AC16G0_03215"
     CDS             2058..>2909
                     /locus_tag="AC16G0_03215"
                     /inference="COORDINATES: protein motif:HMM:NF016292.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098663.1"
                     /translation="MTEAPVKLIWSAVALLLGWLAVLLIPTGVSAQPPQPVARVGILS
                     SGGAQDVRVDYLRDELHKLGYVEGQSLSIMFRWADGRPERLPELAAELVASHVDVIVA
                     IGASVWAAKQQTRTVPIVIAFSGDPVGTGMVSNLARPDGNLTGVSLMSSDLAAKRLEL
                     LTQTVPRTSRFAILYHPAELAAGPELQATEAAARTLGVRLHPLEVRQAEDLEGLFTAA
                     AHGQADGLIVFAHAFAFQHRGPILQLAARHRLPTMYGWREFVDAGGLISYGPSVEAMV
                     RRAAHYVD"
BASE COUNT          505 a          916 c          983 g          505 t
ORIGIN      
        1 ggatcgccct cgcgatcgaa gggttgctgg ggggcggcgt cgaagcccag ccagctcgca
       61 tggtcaagat cggcgccctg acggagtcct ggggacccac gccggccatc atcgggcttc
      121 gggacggcct tacggagctc ggataccggg aaaacgagga tttcgtgatc ggggtgcggt
      181 ttacgcaggg tgatccagct aaactatccg aggccgcccg cgatcttgtt cgacacggag
      241 tcgacatcat cgtaacggct gaagggggaa atactgcgaa ggcggcccag atggctacca
      301 gccggatccc gatcgtgttc atgggcggga gcgacccggt gggcgtggga ttggtacaga
      361 gcttggcaca tcccggcggc aacatcacag gaaccgctga tctggatgtc gaactcgggc
      421 cgaagcggat ggagatcttc cgcgaaatga tccccagcct gaaacggctc ctcatgccct
      481 acgacgcaac gaacacgtac gccgtcgcgc aactggccgt gtaccgggac gccgcccggc
      541 ggctcacgct cacgcttgtc gaaagaccgc ttcggagcga agaagaggca cgggcggcga
      601 tcacggcagt ccggaagggt gaggtggacg gcatcttctc gccacggttc gtctcgctga
      661 acattcccgg cttcatcctc gaaaccgcgg ccaagcgggc gattccgaca atgtttcacg
      721 gagagtttct cgtcgagcgg ggcgggttgg tcagttactc tgccagcgat gagcaattgg
      781 gtcgccaggc ggcgcggctg gtggacaaga tcctgaaggg ggccaagccg ggcgacctac
      841 cggtggagca gccgacgaaa ttcaagctgg tcatcaacct caagaccgcg aaggccctcg
      901 gcctcaagat tccacagtcc gtgctcatcc gagcggacaa ggtgatccag tgaggcaatt
      961 tcggattgcg gatgtcggat tgcgaattgg gccgacgctg gcactcgtgc tcgggctcgc
     1021 tctggctgtc gccggcgcgc catcgctggt ggatgggcaa caggtggagg gggtctctcg
     1081 tgtggggatt gtgctccacg gcggcccgta cttcgcagct ctcgaggggt tgcgggaggg
     1141 acttaaggcg ttggggcttg aggaaggcaa gcgcgtaacc ctcgcagttc gcgacgcgaa
     1201 gggcgatctc aaggcggccg aggcggcggc ccaggcgctt gaacgggagc gggtcagcct
     1261 gatcgtatcc ttcgccacgt cggtctcgct tgcggtgaag cgcggaacgc agcaggttcc
     1321 catcatcttc gtcgcgggaa ccgatccggt cttcgccggg ctggtcgagg gcatcgccaa
     1381 tccgggggga cggctcaccg gcgtccactt cctctcttcg gaactcaccg cgaaacggct
     1441 ggagatcctc aaggaactcc tgccgcggct ccgtcgggtc gtcacgttct acaatccgaa
     1501 caatcttggt gcccagatgt ccgagcggac ggcgcgggaa gccggacaga cactccgggt
     1561 cgaggtcgtt ggacggcagg tgacctcggt ggaggcactc cgggccgcgg tgcgcggtct
     1621 caaggccgaa ccggcggacg cctacttctt cctcgccgac gcgatggtga taagccaggc
     1681 tcaggcgatc attgacgcgg cgaacgccat ccggctgccc accatggccc aagatccgga
     1741 gctcgccgcc aaaggggccc tggcttctta cggcatcagt taccgtgaac tcgggcgcga
     1801 gacggctgcg tatgtccagc ggattctcgc tggcactagc cccagggacc tgccagtcca
     1861 ggcggtcgat cggcttgggc tcgccgtgaa cttcaagacg gccaaggccc tcggcgtcac
     1921 gatcccgcag tccatcctgg tccgggtgga ccaagccatt cgatgaccgg tcgccgatgg
     1981 gtcattgcca gtctcgctct gagtgtcctc gcagctccaa tagccgcgtg ggctgtcttg
     2041 atggctcgat cctcatcgtg acggaggccc ccgtgaagct catctggagc gccgtcgccc
     2101 tgttgttggg ctggctcgcc gtcctcttga tccccacggg agtatctgcg cagccgccac
     2161 aaccggtcgc ccgtgtcggc attctcagct cgggcggggc ccaggatgtg cgcgtggact
     2221 acctcaggga tgagctgcac aaactcggct atgtcgaggg gcagagcctc agcattatgt
     2281 ttcgttgggc cgacgggcgg ccggaacgac tcccggagct agccgccgag ctggtcgcaa
     2341 gccacgtgga cgtgatcgtc gccatcgggg cctcggtctg ggcggcgaaa cagcagacgc
     2401 gcaccgtgcc aatcgtcatc gccttcagcg gcgatcccgt gggcaccggc atggtctcaa
     2461 acctcgcgcg acccgacggg aacctcacgg gcgtatcgct catgtcgagt gatttggccg
     2521 ccaagcggct ggagctgctg acacagacgg ttccacgaac ctcgcggttt gccatcttgt
     2581 accacccggc cgagctggcc gcaggaccgg agctgcaagc gacagaggcc gcggcccgta
     2641 cgctgggcgt gaggctgcac cccctggagg tccggcaagc ggaggacctc gaagggcttt
     2701 tcacggcggc cgcgcatggg caagccgatg ggctcatcgt cttcgcccac gccttcgcgt
     2761 ttcagcaccg cgggccaatc ctccaactgg ccgcccggca ccgtctcccc acgatgtacg
     2821 gctggcggga gttcgtggat gcgggcggac tcatctccta cggaccgagc gtagaggcca
     2881 tggtccggcg agcggcgcac tacgtcgat
//
LOCUS       DBNGZC010000173         5067 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1970215, whole genome shotgun sequence.
ACCESSION   DBNGZC010000173 DBNGZC010000000
VERSION     DBNGZC010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1970215"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="AC16G0_03220"
     CDS             complement(<1..587)
                     /locus_tag="AC16G0_03220"
                     /inference="COORDINATES: protein motif:HMM:NF015013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HIR5098664.1"
                     /translation="MTVDARHVRQIASAAGIVSLATLASRGLGFVRDMAIAWLLGAGL
                     AADAFFVAFRIPSTLRELLGEGALSAAFIPTFTRSATREGREAAWDLASAVLGTLMVI
                     LAGVTAMGVLLAPLIVHILAPGFAHVPGKLQLTVQLLQVMFPYIFLVGLAALFMAVLN
                     SLGHFMTPALSPIMLNLSMIAGALLVAPGSSNPALP"
     gene            757..1035
                     /gene="rpsT"
                     /locus_tag="AC16G0_03225"
     CDS             757..1035
                     /gene="rpsT"
                     /locus_tag="AC16G0_03225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR5098665.1"
                     /translation="MPIIKSSEKDLRRTARRAQRNRAAKGKLRSTLKRVRVALGENQT
                     ESAQRSMREATPVLDRAVTKRILHKNAAARHKSRLMRQLNRLTSPPSA"
     gene            complement(1151..1642)
                     /gene="lptE"
                     /locus_tag="AC16G0_03230"
     CDS             complement(1151..1642)
                     /gene="lptE"
                     /locus_tag="AC16G0_03230"
                     /inference="COORDINATES: protein motif:HMM:NF016290.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HIR5098666.1"
                     /translation="MLSVVCGVLFLGCGYRPLGEGSLPHGIQRLHLAALTNGTFKPGV
                     QGLVGAAILRKLQVDGRVSLASEPDADAILGGTVTTYQNDPITFEQADIGRRFRVRLT
                     LLVTLNDRRGGESVLKEEVSGEAYYTAGTGVVATRTAEDEAMQRAAQDLATKVVARLM
                     DDL"
     gene            complement(1712..4330)
                     /gene="leuS"
                     /locus_tag="AC16G0_03235"
     CDS             complement(1712..4330)
                     /gene="leuS"
                     /locus_tag="AC16G0_03235"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR5098667.1"
                     /translation="MPRGYDFARIEMKWQAAWEARHAFSVTEDPTQPKYYCLEMYPYP
                     SGKIHMGHVRNYSIGDVVARYLGMRGYNVLHPMGWDAFGFPAENAAFEHGVHPAKWTL
                     DNIAYMKTQLQRMGFSYDWGREVACCDPAYYRWNQWFFLKLLERGLAYRRLAPVNWCD
                     RCQSVLSNEQAEGGVCWRHGDTPVVQKELEQWFLKITAYVEELLADLDALTGWPERVR
                     IMQRNWIGKSVGAEVDFPLERGGSIRIFTTRPDTIFGATFMVLAPEHPLALALSAGTF
                     QAAAVAAFAERMRRADKTVRTAATAEKEGVFTGAYATNPLTTERIPIWVANFVLMEYG
                     TGAIMAVPAHDQRDFDFATKYRLPIRVVIQPAGDPLPESGGDRAYEGEGSMVRSGSFT
                     GLPSEEGKLAICRHLEARGIGTQAVNYRIRDWLISRQRYWGTPIPVVYCEGCGVVPVP
                     EQDLPVILPQDVEITMTGGSPLARVEAFVQTTCPRCGKPARRETDTMDTFVDSSWYFL
                     RFTSPHDAARPMDPAKAGYWMPVDQYIGGIEHAVLHLLYARFFTKVCRDLGLTTVREP
                     FANLLTQGMVCKESYRCPEHGYRLPGEVDKQGRCTECQRPVEVGRTEKMSKSKRNVVD
                     PDDLLERFGADTIRLFSLFAAPPEKDLEWSDEGVQGSFRFLTRVFRLVDENAALIAGH
                     RGGAGSGDGQPEAVRNLRRLTHQTIAKVTHDLEDAFHFNTALASLMELQTALGRFQEE
                     SRASTGQGDTPAPATEQLRAFAEGVGALVTMLAPFAPHLAEELWELLGNRESIFRVPW
                     PTADQALAQEEAVEVVIQVNGRVRARLHVPRGMGEDRLKLLALEDTRTRQWLQGKSVR
                     KVIVVPDKIVNVVVAS"
     gene            complement(4538..>5067)
                     /locus_tag="AC16G0_03240"
     CDS             complement(4538..>5067)
                     /locus_tag="AC16G0_03240"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:NF013575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine synthase A"
                     /protein_id="HIR5098668.1"
                     /translation="LDPLRLRRALNASLPGDIVVRAVEEVPENFDARRSARAKTYRYT
                     ILRRDYPSACLRRCSLYVPYALDVEAMAEGARVFLGTHDFSAFRAGTCAARTPVRTIL
                     DASWRQAGDLWHFEIRGNAFLQHMVRIMVGTLLEVGRGRRTPENVASALASRDRRRAG
                     KTAPPHGLCLLEVEY"
BASE COUNT          908 a         1746 c         1597 g          816 t
ORIGIN      
        1 gggagggcgg ggttgctcga gccgggcgcg accagcaggg ctcctgcgat catgctgaga
       61 ttcagcatga tcggggacag ggcgggggtc atgaagtgac ccagagaatt gaggactgcc
      121 atgaagaggg cggccaggcc taccaggaag atgtacggaa acatgacctg gagcaattgc
      181 acggtgagct ggagcttccc cgggacatgg gcaaatcccg gcgccaggat gtggacgatc
      241 agcggcgcca ggaggacgcc catggccgtc acgcctgcca ggatcaccat cagcgtgcct
      301 aggacggccg aggccagatc ccaggccgcc tcccgccctt cgcgggttgc gctccgggtg
      361 aaggtgggga tgaaggcggc agagagggca ccctcgccca ggagttcgcg gagggtgctg
      421 ggaattcgga aagccacgaa gaaggcgtcc gccgcgagcc ccgcacccag gagccaggcg
      481 atggccatgt ctcgaacgaa gccgagcccc cggctcgcca aggtggccag gctcacgatc
      541 ccggcagccg aggcaatctg cctgacgtgt cgggcgtcca cggtcatggt cgcaccatcc
      601 agggatctcg tttaatcgca cccctccacg gccaacaccc ttccgcccca gaaagccctt
      661 gacatcctct gcccgctcct ctataagttc aacgccctct ggccagcagt cgcggccgtc
      721 cggcacgttt cgtcaccgtg gaaaggagtt gactgtatgc caatcatcaa gtcgtcggag
      781 aaggacttgc ggcgcacggc ccgccgggcg cagcgcaacc gggccgccaa gggcaagctc
      841 cggtcgactc tcaagcgcgt gcgggtcgcc ctcggggaga atcagaccga gagcgcccag
      901 cgatccatgc gcgaggccac ccccgtcctg gatcgagccg tcacgaaaag gatccttcac
      961 aagaacgctg ccgcccggca caagtcccgc ttgatgcggc aactgaaccg actgaccagc
     1021 ccgccttccg cctgatcggc cttcgcctgc acgaggccgg gcgactcggc cgccggaccc
     1081 ctcttccgcc ctcgattcca gctcaagggc tcatcccgct tcagcttacc cgtttgagcc
     1141 tcgaagatcg tcacagatcg tccatcagcc gggcgacgac ctttgtcgcg agatcctgcg
     1201 ccgcacgttg catggcctca tcctcagccg tccgggtggc cacgacaccc gtcccagcgg
     1261 tgtaataggc ctccccggaa acctcttctt tcagcacgct ctcgccaccc cggcgatcat
     1321 tgagggtgac cagcagcgtc aatcgcacac ggaaccggcg cccgatgtcc gcttgctcga
     1381 aggtgatcgg gtcgttttga taggttgtca ccgtgccccc gaggatcgca tcggcatcag
     1441 gttcggaggc gaggctcacg cggccgtcca cttggagctt ccgcaggatg gccgcaccga
     1501 ccaacccctg gacgccgggc ttgaacgtcc cattggttag ggcggccagg tggagtcgct
     1561 ggatcccgtg ggggaggctc ccctcgccca ggggccgata gccgcacccg aggaagagga
     1621 ccccgcacac aacagaaagc acgaggagag gaatggcgcg ggagaatcca tgcatgcaga
     1681 atcgcactct cgagcccacg gaagagatcg ctcaggaagc cacgacgacg ttgacgatct
     1741 tgtccgggac cacgatgacc ttgcgcacgc tctttccctg taaccactgc cgggtacggg
     1801 tatcttcgag ggccaggagc ttcaaccgat cctcccccat tccacggggg acgtgcagcc
     1861 tggcccggac cctgccattc acctggatga ccacctccac cgcctcctct tgggcaagcg
     1921 cctggtccgc ggtgggccac ggcacgcgga agatgctctc ccgattccca agcagctccc
     1981 acaattcctc tgccaggtgc ggggcgaagg gggccagcat cgtgaccagg gcgccaaccc
     2041 cctccgcgaa tgcgcgtagc tgctcggtcg ccggcgcggg cgtgtcgcct tggcccgtcg
     2101 aagcccgcga ttcttcctga aagcgtccca gggcggtctg cagctccatc aggctcgcca
     2161 gcgccgtgtt gaaatgaaag gcatcctcca aatcgtgggt gaccttggcg atcgtctggt
     2221 gggtgaggcg ccgcaggttc cggaccgcct cgggctgacc gtcccccgat cccgcgccgc
     2281 cccggtggcc ggcgatcagg gccgcattct catccaccag gcggaagacc cgggtgagga
     2341 accggaagga gccctggacc ccctcgtccg accattccag gtccttctcc ggtggcgccg
     2401 cgaagagtga gaagaggcgg atcgtgtcgg ccccgaagcg ctccaggagg tcatcggggt
     2461 ccaccacgtt gcgcttggac ttggacatct tctcggtccg gccaacctcg accgggcgct
     2521 ggcactcggt gcagcggccc tgcttgtcca cctccccggg caggcggtaa ccgtgttcgg
     2581 gacagcggta gctctccttg cagaccatcc cctgggtcag gagatttgca aagggttccc
     2641 gcaccgtggt gagtcccagg tcccggcaga ctttggtaaa gaagcgggcg tagagcaggt
     2701 ggagcaccgc gtgctcgatc ccgccgatgt actgatccac tggcatccag tagcccgcct
     2761 tggcgggatc catggggcgc gccgcgtcgt gggggcttgt gaaccggaga aaataccacg
     2821 aggaatccac gaaggtgtcc atggtgtcgg tctcgcgccg cgccggcttt ccgcacctgg
     2881 gacaggtcgt ctgcacaaaa gcctccaccc tggccagggg cgagcccccg gtcatggtga
     2941 tctccacatc ctgggggagg atcacgggga ggtcctgttc gggtaccggg accacgccgc
     3001 acccctcaca gtacaccaca gggatcgggg tgccccagta acgctgtcgg gagatcaacc
     3061 agtcgcggat ccgatagttc accgcctgcg tgccgatccc gcgggcctcc aggtgacggc
     3121 agatcgccag cttcccctcc tcgctcggca ggccggtgaa gctgccggac cggaccatgc
     3181 tcccctcgcc ctcgtacgcc cgatcccctc ccgactctgg caggggatcg cccgccggct
     3241 ggatcaccac ccggatcggc aagcgatact tcgtggcgaa atcgaaatcg cgctggtcgt
     3301 gagccggcac cgccatgatg gcccctgtcc cgtactccat gaggacgaag ttcgccaccc
     3361 agatcgggat gcgctccgtc gtcagggggt ttgtggcata ggcgccggtg aagaccccct
     3421 ccttctcggc cgtggcggcc gtccggacgg tcttgtcggc ccgtcgcatc cgctcggcga
     3481 aggccgccac ggccgccgcc tggaatgttc ccgccgacag ggcaagggcc aggggatgct
     3541 ccggcgcgag caccatgaag gtcgcgccga agatggtgtc gggccgcgtg gtgaagatcc
     3601 ggatgctccc accccgttcc aggggaaaat ccacctcggc acccacgctc ttcccgatcc
     3661 agttgcgctg catgatgcgg acgcgctcgg gccaacccgt cagcgcgtcg aggtcggcca
     3721 ggagttcctc cacatacgcg gtgatcttca agaaccactg ctctagctct ttctgcacga
     3781 cgggcgtgtc gccgtgacgc cagcagacgc caccctcggc ctgctcgttg ctcagcaccg
     3841 actggcagcg gtcgcaccag ttcaccggcg ccaggcggcg gtaggccagg ccccgctcca
     3901 agagcttcag gaagaaccac tggttccagc ggtagtaggc gggatcgcag caggcgacct
     3961 cgcgccccca gtcgtaagag aaccccatgc gctggagctg agttttcatg taggcgatgt
     4021 tgtccagggt ccacttggcg ggatgcaccc cgtgctcgaa ggccgcattc tcggccggga
     4081 agccgaaggc gtcccatccc atgggatgca ggacattgta cccccgcatg ccgaggtagc
     4141 gggccaccac gtcaccgata gaatagttgc gcacgtgccc catgtggatc ttgcccgagg
     4201 ggtagggata catctccaga cagtaatact tcggctgcgt cgggtcctcg gtgacactga
     4261 aggcgtgacg cgcctcccat gcagcctgcc acttcatctc gatgcgtgca aaatcatacc
     4321 cgcgcggcat ccgaaccctc cggtgagatg ttggccttat ctaacacggc tgttctgcct
     4381 tggcaagggc gcccaggcct tgagacgcgt cccctcaggc gccggccagc ggcattcgcc
     4441 acgaccaggt cgaaacgcag ggcatgccgc ccgcgtgact cggcacgacc agcggaccag
     4501 atgcttccag cccgaatcca gccagggttg gaagccgtca atactcaacc tccagcaagc
     4561 acaaaccgtg agggggtgcc gtttttccgg cccgccgccg atcccgggac gccaaagccg
     4621 atgccacgtt ttccggtgtc cgtcggcccc gtccgacctc cagcagcgtg cccaccatga
     4681 tccgtaccat gtgctgcaga aaagcgttcc ctcggatctc gaagtgccac aggtcacccg
     4741 cctgccgcca ggaggcatcc aggatggttc gcaccggcgt tctggccgcg caggtccccg
     4801 cccggaaggc cgagaagtcg tgggtgccca ggaagacccg agcgccctcg gccatcgcct
     4861 ccacatccag ggcgtagggc acatacaggc tgcatcggcg caggcaggcc gagggataat
     4921 cccgacgaag gatcgtataa cgataggtct tggcccgggc agatcgtcgg gcatcgaagt
     4981 tctcgggcac ctcctccacg gcgcgaacca cgatgtcccc cggcagactg gcgttcagcg
     5041 cccgtcgcag ccgcagggga tccagcc
//
LOCUS       DBNGZC010000174         3129 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2068499, whole genome shotgun sequence.
ACCESSION   DBNGZC010000174 DBNGZC010000000
VERSION     DBNGZC010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2068499"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..161)
                     /locus_tag="AC16G0_03245"
     CDS             complement(<1..161)
                     /locus_tag="AC16G0_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007487901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HIR5098669.1"
                     /translation="MPFEDKTLTCVDCGQTFVFTAGEQEFFAQKGFQNAPKRCKSCKT
                     TKRGGDRPGG"
     gene            complement(262..1401)
                     /locus_tag="AC16G0_03250"
     CDS             complement(262..1401)
                     /locus_tag="AC16G0_03250"
                     /inference="COORDINATES: protein motif:HMM:NF013046.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HIR5098670.1"
                     /translation="MADPLQTAFIYSERYGQFDYGPEHPFRIERLALTHDLCQAYGLL
                     TLPATRLMHARDATEEEVAAFHRPDYVEVLRAADRGQAPPNVWDYGLGTGDNPIFPGM
                     YAWSLLTTGASLLAMEAVDTGEVRCAFNLAGGLHHAAAARAAGFCYFNDAAVIIKHLV
                     RRGRRVAYVDIDAHHGDGVQFGFYDTDQVLTISLHESGETLFPGTGFVEEIGRGRGIG
                     YAANVPLLPGSDDEIFLWAFDQVVPPLLAAFHPDVVVTQLGIDTHHTDPLAHLGLTTN
                     GFGEAVRRLKALCPKWIALGGGGYNVENVPRGWTLAWAIMNDREIPDELPAAFLETFR
                     RLGFSHSRIRDEPHVADPSVRDRTWRFAREQVRRLQRLVFPHHGI"
     gene            complement(1516..1968)
                     /gene="moaC"
                     /locus_tag="AC16G0_03255"
     CDS             complement(1516..1968)
                     /gene="moaC"
                     /locus_tag="AC16G0_03255"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR5098671.1"
                     /translation="MVDISAKDETLREAVARGFVALRPSTLRLIRDRALPKGDVYAAA
                     RLAGILAAKRVHELVPLCHPLLITSAEIALWATTRPSGVEIESRVKTTGRTGAEMEAL
                     AAVSVAALTIYDMCKAVDKEMVVGNVRLMFKTGGRSGTYHRPREASPK"
     gene            complement(1988..2236)
                     /locus_tag="AC16G0_03260"
     CDS             complement(1988..2236)
                     /locus_tag="AC16G0_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HP1242/YdcH family protein"
                     /protein_id="HIR5098672.1"
                     /translation="MSDTEAQLFTRLKEENPEFRRLAEKHREFDVKIGEYDRIYYLTS
                     EQERKRKELQKLKLSIKDKMYAIMRQYKNEQQPQTVSS"
     gene            2598..>3129
                     /locus_tag="AC16G0_03265"
     CDS             2598..>3129
                     /locus_tag="AC16G0_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098673.1"
                     /translation="MGSTFGQRLLRFKACGLRVLISLIGVSLLVGCGSTPPGPQTPPE
                     IRTLEADLDLACEAAIRVLEDRGYDLPRTDRTAGVIQSGWLSTNSRYAASIFVTEHRD
                     RYSDCGKPGLGRAFRGKETRLEVMLSPNGRGETSLRIDAAFRTQRFFSFLWWNRPLGD
                     WPCPSRGRLEEEIALQM"
BASE COUNT          553 a         1003 c          988 g          585 t
ORIGIN      
        1 ccaccgggtc ggtcgccgcc tcgcttggtg gttttgcagc tcttgcaccg cttcggcgcg
       61 ttttgaaacc ccttctgggc gaagaattcc tgttccccgg cggtaaagac aaaggtctgg
      121 ccgcaatcca cacaggttaa cgtcttgtct tcgaacggca ttcggagtcc tccctcggca
      181 aatgatccca gactgcaccc tcgatccacg ccctggttgt gtccgtggct accctcctgt
      241 gatgtgggaa tcccgacatg ctcaaatccc gtggtgcggg aaaaccagcc gttgcaaacg
      301 gcggacttgt tcgcgggcga agcgccaggt gcgatcacgg acggatgggt ccgcaacgtg
      361 aggctcgtcc cggatccgcg agtgggagaa tcccaggcgg cgaaaggtct ccagaaaggc
      421 tgccggcaat tcgtcgggga tctcgcggtc attcatgatg gcccaggcca aggtccagcc
      481 ccgcggcacg ttttctacgt tgtacccccc gccccccagg gcaatccact tcgggcagag
      541 cgccttgagc cgccgcaccg cctcgccgaa accgttcgtc gtcaggccca gatgcgccaa
      601 cggatcggta tggtgggtgt cgatgccgag ctgggtcacc accacatccg ggtggaaggc
      661 agccaagagc ggcggcacca cctgatcgaa ggcccacagg aaaatctcgt cgtcgctccc
      721 gggcagcagg ggaacatttg ccgcgtagcc gatgcctcgg ccccggccaa tctcctccac
      781 gaaaccggtg ccggggaaga gcgtctcgcc ggactcatgc agggagatcg tcaagacctg
      841 gtccgtgtcg tagaatccaa actgcacccc gtcgccatgg tgcgcgtcga tgtccacgta
      901 cgccactcgg cgcccgcgac gaaccaggtg cttgatgatg acggcggcat cgttgaaata
      961 acagaagccg gccgcacggg ccgcggccgc gtggtgcagt ccgccggcca gattgaaggc
     1021 acagcgcacc tcgccggtat cgaccgcttc catggccagg agcgatgccc ccgtggtgag
     1081 cagggaccag gcgtacatgc ccggaaagat cgggttgtcg ccggttccca gaccgtaatc
     1141 ccagacgttg gggggcgcct gtccgcgatc cgccgcccgg aggacctcca cgtagtccgg
     1201 ccggtggaag gccgccactt cctcttccgt ggcatcccgg gcatgcatga ggcgtgtggc
     1261 aggaagggtg aggaggccgt acgcctggca gaggtcgtgg gtgagcgcca agcgctcaat
     1321 gcggaagggg tgctccggcc catagtcgaa ctgtccgtac cgctcggagt aaatgaacgc
     1381 cgtctggagg ggatcagcca tcagccatca gccatcagcg gtcagccttg aactcgcatg
     1441 cctcggatct gatcagtcac tccgcccgcg cgcgccggtc ctcctcgctg aaggctggtt
     1501 gctgaaagct gcaggctact ttggggacgc ctcccgcggc cggtggtagg tcccgctccg
     1561 cccgccggtc ttgaacatca gacggacgtt gccgacgacc atctctttgt ccactgcctt
     1621 gcacatatcg taaatcgtca gggcagccac ggagaccgcg gccaaggcct ccatttcggc
     1681 cccggtccga ccggtggtct tgacccgcga ctcgatctcc acgccagagg gccgggtagt
     1741 cgcccacagc gcaatttccg ccgacgtgat caggagggga tggcagagag gaaccagttc
     1801 gtgaacccgc ttggccgcca ggatacccgc caggcgcgcc gcagcgtaca catctccctt
     1861 cgggagggcg cggtcgcgaa tcagacgcag ggtactcggc cggagcgcca cgaacccgcg
     1921 agccactgcc tcacgaaggg tttcatcctt ggcgctgatg tccaccatct gcgcccggcc
     1981 gcgagcatca agatgagaca gtctggggtt gctgctcatt cttgtactgg cgcatgatgg
     2041 cgtacatctt gtccttgatg cttaatttca gcttttggag ttccttgcgc ttgcgttcct
     2101 gctcactggt gagatagtaa atgcggtcat attcgccaat cttgacgtcg aactcgcggt
     2161 gtttctcggc caggcggcga aactcgggat tttcctcctt gagccgagtg aacaactgcg
     2221 cctccgtatc gctcatcttt gacctccctt ccgggaatgg gaagaaatag aaataggaat
     2281 cggtccgctc cgcgcccagg tgaggcagcg tgggacctga cgaagataga gtggtttggg
     2341 taggcttggg gactcgatgc ggacggtcgt ggtcgcgggg ccgatccggc cccgcatgta
     2401 agggcagacg atcaccatat ttgatttggg ttaaaaccca ggtgcaggat attcgatcag
     2461 ctagcctgcg tcaaggccaa aatcccccgg cgctccatcc acagcctgat gcccccggtt
     2521 tgcctgcctt cggcttgaca agcatcgtct ctcccggata ctgttcctgg gttctggcgc
     2581 ctggcccagg agaatccatg ggaagcacct tcggccaacg cttgctccgg ttcaaggcct
     2641 gcggcctgcg ggtgctgatc agcctgatcg gcgtgagcct gctggtgggc tgcggatcga
     2701 cgccgccggg gccccagact ccccccgaga ttcgcacgct cgaggccgac ttggatctgg
     2761 catgcgaggc cgccatccgg gtgctggagg accgcggcta cgaccttccc cggacggatc
     2821 gaacggcggg ggtcatccag agcggctggt tgagcaccaa ttcccgctat gccgcttcga
     2881 tcttcgtgac ggagcaccgg gatcgctaca gcgattgtgg aaagcccggg ctgggccggg
     2941 ccttccgcgg caaagagact cgcctggagg tgatgctctc ccccaatggc cggggcgaga
     3001 ccagcctccg gattgacgcc gcgttccgca cccagcgctt cttcagcttc ctctggtgga
     3061 atcggccgct gggagactgg ccttgcccct cccggggtcg tctggaggag gagatcgcgc
     3121 tgcagatga
//
LOCUS       DBNGZC010000175         2732 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_894773, whole genome shotgun sequence.
ACCESSION   DBNGZC010000175 DBNGZC010000000
VERSION     DBNGZC010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_894773"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="AC16G0_03270"
     CDS             complement(<1..194)
                     /locus_tag="AC16G0_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098674.1"
                     /translation="MPLVVAVPVLVMSPPVLTLPLPFPLPLVCPRRWSMMLPVRRSVS
                     VVLDRLNLYRPRDLRDGNGRP"
     gene            complement(506..865)
                     /locus_tag="AC16G0_03275"
     CDS             complement(506..865)
                     /locus_tag="AC16G0_03275"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR5098675.1"
                     /translation="MPLERRRPDHVRVPLRISALLFEEATPSRRPTPGETRDISRGGA
                     RLRVEGATRPSTPVEVILRLVHRPPLSLSGTVVWSAGPPDPSGRELGIQFREEIPPGL
                     VAEIAHAGCRGSPGPAE"
     gene            complement(1845..2288)
                     /locus_tag="AC16G0_03280"
     CDS             complement(1845..2288)
                     /locus_tag="AC16G0_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098676.1"
                     /translation="MKAHTEWTLEELWQQEQINFSAHLAIMFRYLEKQGLGLDEFISF
                     VGGSVLPSWKRRGDTIPIVMNGILRNVLANGGAILNVTMGEDRAEAPRTSLLRLDVMD
                     RYGIPEDKASRFWDKFVPIAAALGMRFSWERTAAGQDRIRLEKLG"
     gene            complement(2478..>2732)
                     /locus_tag="AC16G0_03285"
     CDS             complement(2478..>2732)
                     /locus_tag="AC16G0_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR5098677.1"
                     /translation="TLAKGNAFLASTDRVAIDAVGVAILKHLGSNEKIMQPKIFEQEQ
                     IARAVELGLGASAPDQIQLVAVDDKTREYRNKIEAILRQG"
BASE COUNT          480 a          899 c          869 g          484 t
ORIGIN      
        1 ggacggccat tgccatcgcg caggtcccgc ggccgataca ggttgagccg gtcgaggact
       61 accgaaaccg acctgcgcac tgggagcatc atggaccacc gccgtgggca cacgagcggc
      121 aacgggaacg ggagtgggag cgtcagcacg ggcggggaca tgaccagcac ggggaccgcc
      181 acgaccaggg gcacgggcag gatcgggact agcagtcccc gcgacaggca agccttcacg
      241 ggcgccgagt gaagtttctc ggcgctcgtg ctgtttatag gcgaccgctc gatgtgagga
      301 cgagtggccc tgcggacgct cagcgtgagc aggagggagg cttcgagttg ctcctctgcc
      361 ggcaggccat ccagcgattc cagggcgagt gaatgcgggc cagccagggc gacgcctggc
      421 caagcgaacg gcagagcgcg ggacctctcc aggcaccgag gatgtgcggt ccccggcctt
      481 cctcggtgcc gaccggccct ccggattact cggcaggccc aggcgagccc ctgcaccccg
      541 cgtgggcgat ctcggcgacg agcccagggg ggatctcctc ccggaactgg atgcccagct
      601 cccgcccgga cgggtcgggc ggtcctgcgc tccacacgac ggttccactg agggagagcg
      661 gcgggcgatg gaccagccgc aagatcacct ccactggggt gctgggacgc gtcgcccctt
      721 ccacccggag gcgggccccg ccccggctga tgtcccgggt ctcacctggc gtggggcgcc
      781 gcgatggggt ggcctcctca aacagcagcg ccgaaatgcg gaggggcacc cggacatgat
      841 ccggtcgacg acgttcgagt ggcatgacga ccctcctcgg acctgttgga tcacacgcag
      901 tccgtccatg ccatgagcga tctggcagcc ccccgaaccg cctgattcgc atgcctcgtt
      961 ggtccgccca aagcattccg cagcgtagca gcttcccgtt cacgttgaat cacccgagcg
     1021 tgcgccagac aacgtcgtcc tctgcccctc atatcagcct tggcagcgcc gctcccgtcg
     1081 gctcgtcggg cgtcttcccg acgaggcaga gccccgtcca ctgacctggt gccggtggag
     1141 gcttgtgcga ccccaccagg ctggcggtcc agacctctcc gattggatat acgacgaggc
     1201 ggacccagtg cggctcgtcg ctgggttcga tcgcgaggag atgagggctg cgctcgggca
     1261 tgttgcaccg gtcagccacg tgccaagggg aatgcgacag tacgagagct aagaagcaag
     1321 ggccagcatg gcccagcatg cgcagaaggt actgtggtgg gcgagccgtc tcccagtggt
     1381 ggggtaggcg caggaaggct caaaaggcac cctagcgaac aggtgccctt gcgtcaagag
     1441 gatcgtgagg atcgagtgaa aaccctggaa ggagcacgat tcatcgaaat aataattgct
     1501 cactgcgtac ggagtcacgc gccattcgga ttccgccgtg ttgcggaact cggatagcgg
     1561 ggtcgtactg ctatagcgga cctctggtgt cgagacacca ctgcgaagcc atatgccctg
     1621 gcggtatgcc acggcacgga gaaggtgacc agccctctcc gctccagtag gttcggcagg
     1681 gactcgaacc cgaagggctg ggtcaatccc acgtcttcca ggggcaccgc ttgcttcccg
     1741 cccgcctcaa cggcctgggc cacgcggcgg aggagccaag actcgaccgc accgacggcg
     1801 ccgagggcgt gattgattat gccagcaatt tccgcagcag cgcgtcaccc cagcttctcc
     1861 aggcggatcc gatcctgccc ggcagcggtg cgctcccagg agaaccgcat gcccagggcc
     1921 gcggcgatgg gcacgaactt gtcccagaag cggctggcct tgtcttcggg gatgccgtag
     1981 cggtccatca cgtcgaggcg caagagactc gtccgcggtg cctcggctcg gtcttctccc
     2041 atggtcacgt tcaggatcgc acccccattg gccagcacgt tgcggagaat cccgttcatc
     2101 acgatgggaa tggtgtcgcc ccggcgcttc caactgggga ggacgcttcc cccgacgaag
     2161 ctgatgaact cgtccaggcc caggccctgc ttctccaggt agcggaacat gatcgccaag
     2221 tgggccgaaa agttgatctg ctcctgctgc cacaattcct ccagcgtcca ctcggtatgg
     2281 gccttcaagg catcctccta tttctatttc ggctgggttc ggtggcaccc cactgcgtcc
     2341 gactgtatgc tcggcgcagg tccagccgaa tcttttccaa gcgttgcact ttagacagct
     2401 actgcatgtt gcgcggatcg cgcccgccag cccttcgcat cggcctctgc cactcacctc
     2461 tcatttcacc gggctggtta tccttgtcgc aatattgctt ctatcttgtt ccgatattcc
     2521 cgggttttgt cgtctacggc caccagttga atctggtccg gcgcggatgc acccaatccc
     2581 agctctacgg cccgggcgat ctgttcctgc tcgaagatct tcggctgcat gatcttctca
     2641 ttgcttccaa ggtgtttgag gatggccacc ccgaccgcgt caatggcgac tcgatccgtc
     2701 gacgcgagga acgcatttcc cttggcgagc gt
//
LOCUS       DBNGZC010000176         3438 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2264155, whole genome shotgun sequence.
ACCESSION   DBNGZC010000176 DBNGZC010000000
VERSION     DBNGZC010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2264155"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1462
                     /locus_tag="AC16G0_03290"
     CDS             152..1462
                     /locus_tag="AC16G0_03290"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR5098678.1"
                     /translation="MSETLAIRGGTPVVPKALHRRWPVITDEDRAAVLDVLEAGILNG
                     PYAPQVKALEEEWARYCDVRYAITTNSGTAALHTAVAAGQIGTGAQVITTAFTFLATA
                     LAVLQNNAVPVFVDIDPRTYNIDPAKIAERITSRTRAIIPVHIHGLPADMDPILAIAK
                     KHGLLVIEDAAQAHGSTYKGKKVGGIGDMGIFSFQASKNLPAGEGGIFVTNREELKER
                     ANRFRMFGEDVRESDKKAFDPARPLDGAREYNSLMMGWMYRATELTAALCRSQLRRLD
                     AWLANTRLNAAYLSEHLGKIPGITPPHIPPDCTSSFYQYRIRLDPRAAGSDLPPKEFR
                     LKVLAALKAEGVEVSLWQTVPVPGQTLFQERTGYGLSCPWKCPLGEEVKYDLAEYPET
                     LRLLEDSMVVGSHSYPLFPQPMALMEAYVKAFQRVFTNWKLALA"
     gene            1627..2580
                     /locus_tag="AC16G0_03295"
     CDS             1627..2580
                     /locus_tag="AC16G0_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5098679.1"
                     /translation="MKSQYTGTATDQVLCGFRERPLRRWHVHLRFEALTAWGFLAPTV
                     LLLALLAAYPLAYSLYLSLTTTQVGAIGRFVGAQNFVQLLHTEIFTLTLSNTIWYTVL
                     AVGAKLVLGLILALVLTQQAPGMKWIRGAILIPWVAPVSLSVLAWTWMFDSTYSVINW
                     GLEHLGLISTPVAWLGIPRLARFAVVLVNVWSGLPFFAINFLAGLVTIPRELHEAATV
                     DGAGPLVRFRSITLPLLRPVLATVLLFSVVMTSSDFATVFVLTHGGPMNSTQVLATLA
                     FKLGLATGDLGQAAAISLYLFPLLALATVVQVRLMRRTWQW"
     gene            2574..3416
                     /locus_tag="AC16G0_03300"
     CDS             2574..3416
                     /locus_tag="AC16G0_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5098680.1"
                     /translation="MVRGRTRSVVIAYLTAAPFVLFAVFPFAWMLITSLKKNSELYDL
                     SQNPFWVRNGITWEHYRFLLERTPFPRWLENSTLVGVVTTIVSVVFALLAAYALTRLR
                     FRGAEAVATSIFIVYLVPTTLLFLPLARIVAVLGLSNSLWALILTYPAFIVPFATWML
                     MAYLSSIPRDLEESALVDGCTRLRAIWHIVLPLARPGIVTAALFSFTLSWGEFIYALT
                     FISTTDLKTVTAGVVVNLIRGDVFYWGSLMGGALLASLPVVLLFSLFLDSYVSGLTAG
                     AVKG"
BASE COUNT          539 a         1173 c         1076 g          650 t
ORIGIN      
        1 aggcagtccg gcaggccatc gcccagatgg aacaggccgt gcagcacgtc ttctccacca
       61 ccgtcgtctc ccatacgctg gtgatccgct cctcgtgcgg caccggcgtg cggcccgtgc
      121 agcaagcccg ccgtagtgga aggagggtgc catgagcgag acactggcga tccgaggcgg
      181 gacccccgtg gtccccaaag cgctgcatcg ccgttggccg gtgatcaccg acgaggaccg
      241 ggcggccgtc ctggacgtcc tggaggccgg catcctgaac gggccctacg cgcctcaggt
      301 gaaggccctg gaggaggaat gggctcgcta ctgcgacgtg cgctacgcca tcacgaccaa
      361 tagcgggacg gcggcgctac acaccgcggt ggccgccggc cagatcggga ccggtgctca
      421 ggtgatcacc acggccttca ccttcctggc cacggccctg gcggtcctcc agaacaacgc
      481 ggtcccggtc ttcgtggaca tcgacccccg cacctacaac atcgatccgg cgaaaattgc
      541 ggaacggatc acgtcgcgga cccgggcgat catccccgtc cacatccacg gtctccccgc
      601 ggacatggac ccgatcctgg ccatcgcaaa gaagcacggc ctactggtca tcgaggacgc
      661 ggcccaggct cacggctcca cctacaaggg caagaaggtc ggagggatcg gtgacatggg
      721 catcttctcc ttccaggcca gcaagaacct gcctgcgggc gaaggaggca tcttcgtcac
      781 gaaccgagag gagctgaagg agcgcgccaa tcgcttccgg atgttcgggg aggacgtccg
      841 ggaaagcgac aagaaggcct tcgacccggc ccgtcctctg gacggggcgc gcgagtacaa
      901 ttcgttgatg atgggctgga tgtaccgtgc caccgagctg accgcggccc tgtgtcgcag
      961 ccagttgcgc cgcctcgacg cctggctggc caacacgcgg ctgaacgccg cgtacctcag
     1021 cgagcacctg ggaaagattc cgggaatcac cccgccccac attccgcccg actgcacctc
     1081 gagcttctac cagtaccgga tccgcctgga tcctcgggcc gccgggtccg atctcccgcc
     1141 caaggagttc cggctgaagg tgctggccgc cttgaaggcc gagggggtcg aagtcagcct
     1201 ctggcagacg gtccccgtcc cagggcaaac gctgttccag gagcggaccg gttacgggct
     1261 gtcctgcccg tggaaatgtc ccctgggcga agaggtgaag tatgatctgg ccgaataccc
     1321 cgagacgctg cgcctgctgg aagactccat ggtcgtcggc tcccactcct accccctctt
     1381 cccccagccg atggcattga tggaggccta cgtaaaggcg ttccagcggg tattcaccaa
     1441 ctggaagctg gcgttggcat gaggcggagg ctccggctga cagagcctgc accggttgcg
     1501 agagatcagc ctgtgaagct ctggggcttg gttattcccc cggcacccgc aagacggaag
     1561 acggaacctg gcgagtttct agcgcgctga gacgaacggg ccacgtccag cagtgtgccc
     1621 tttccgatga agagccaata cacggggacg gcgaccgatc aggttctgtg cggattccgc
     1681 gagcggccgc tgcgcaggtg gcacgtgcac ctgcgcttcg aggcgctcac ggcgtggggt
     1741 tttctcgcgc ccacggttct gctcctggcc ttgcttgccg cctacccgct ggcgtattcc
     1801 ctctacctga gtctgaccac cacccaggtg ggcgccatcg gccgcttcgt gggcgcgcag
     1861 aacttcgtgc aattgctgca caccgaaatc ttcaccctca ccctctccaa caccatctgg
     1921 tacaccgtgc tggccgtggg ggccaagctg gtcctggggc tcatcctggc gctggtcctg
     1981 acgcaacagg ctccggggat gaagtggatc cggggggcga tcttgatccc ttgggtggcc
     2041 cccgtctctc tcagcgtgct ggcctggacc tggatgttcg actcaaccta cagcgtcatc
     2101 aactggggcc tggagcatct cgggctcatc tccacgcccg tcgcctggct ggggataccg
     2161 cgtttggcca gattcgcggt ggtcctggtg aatgtctggt caggcctccc cttcttcgcc
     2221 atcaacttcc tggccggtct cgtgaccatc ccgcgcgaac tccacgaggc ggccacggtg
     2281 gacggggccg ggcccctggt gcgcttccga tccatcaccc tcccgctgct gcggccggta
     2341 ttggccacgg tgttgctctt ctcggtggtc atgaccagca gcgactttgc cacggtgttc
     2401 gtcctgacgc acggggggcc gatgaacagc acacaggtcc tggcgacgct ggctttcaag
     2461 ctgggcctgg cgacgggcga cctggggcag gccgcggcca tctccctcta cctgttccct
     2521 ctcctggctc tggcgacggt ggtccaggtg cggctgatgc ggaggacctg gcaatggtga
     2581 gagggcgaac gcggtccgtg gtcattgcct acctcacggc ggcccccttt gtgctcttcg
     2641 ccgtgttccc gttcgcctgg atgctgatca cgtcgttgaa gaagaacagc gagctgtacg
     2701 acctctccca gaaccccttc tgggtgcgga acggcatcac ctgggagcat taccgcttcc
     2761 tgctggagcg gaccccgttc ccgcggtggc tggagaattc aaccttggtc ggcgtcgtga
     2821 ccaccatcgt ctccgtggtc ttcgccctgc tggccgcgta cgccctgacg cggctccgct
     2881 tccgcggggc cgaggcggtg gccacctcca tcttcatcgt gtacctggtc cccaccacgt
     2941 tgttgttcct tcccctggcg cggatcgtcg cggtgctggg cctctccaac agcctgtggg
     3001 cgctcatcct gacctatccc gccttcatcg tcccgttcgc cacctggatg ctcatggcct
     3061 atctctccag cattcctcgc gacctggagg aaagcgccct ggtggacggc tgcacccggc
     3121 tgcgcgccat ctggcacatc gtcctcccgc tggcacgacc cggcatcgtc acggcggcgc
     3181 tcttttcctt cacgctgtcc tggggagagt tcatctacgc cctgacgttc atctcgacca
     3241 ccgacctaaa gacggtgacc gccggggtgg tcgtgaacct cattcgcggc gacgtgttct
     3301 actggggatc cctgatgggc ggtgccctcc tcgcctccct gcccgtggtc ttgctgtttt
     3361 ccctgttcct ggactcgtac gtttctggtc tgaccgctgg ggccgtgaaa ggctagagag
     3421 gaggtgaacg gcggtatc
//
LOCUS       DBNGZC010000177         1451 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1970683, whole genome shotgun sequence.
ACCESSION   DBNGZC010000177 DBNGZC010000000
VERSION     DBNGZC010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1970683"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..480
                     /gene="gatC"
                     /locus_tag="AC16G0_03305"
     CDS             187..480
                     /gene="gatC"
                     /locus_tag="AC16G0_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003335653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR5098681.1"
                     /translation="MAKITRKEVEHVARLARLELEEEEKERMTAQLDSILGYIDKLNE
                     LDTTHVEPTTTVIPMVSVMREDVVRPSLSQDEALANAPDREDVYFRVPRIIED"
     gene            505..>1451
                     /locus_tag="AC16G0_03310"
     CDS             505..>1451
                     /locus_tag="AC16G0_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR5098682.1"
                     /translation="MDLFRLTIHELRDLIQKGEISPVQVVHNYLVRIGEVEDRVQAFN
                     TLTPRTALAFAGKFAKMQAEKKPLPPLAGIPLAIKDVICTKGVTTTCSSKILESYIPP
                     YNASVVNRLNASGALMLGKTNMDEFAMGSSTENSASKQTRNPWDLGCVPGGSSGGSAA
                     AVAADMCAGALGTDTGGSIRQPGGFCGVAALKPTYGRVSRYGLVAFASSLDQIGPITK
                     DVRDAAILLKAIAGHDPLDSTSAELPVADYETALTGDIRGLRIGIPAEYFIPGMDPEV
                     EAAIRGAIQVLEGLGGIQESVSLPHTGYAVAAYYLVATA"
BASE COUNT          279 a          444 c          510 g          218 t
ORIGIN      
        1 atcggaatca tcaaggaacg catcggggag ggcgaagacc tgaaggtggt cgtcaccttc
       61 gcaggcgtgg gccggaagaa gctggcagcc cagttcgttc acctggagcg ggcatagaaa
      121 agagaaagct gtcgatattc ggcggcggtt ctctcggggg gctgaccgcg ggacaagggg
      181 gcaccggtgg cgaagatcac gcgcaaggaa gtcgagcacg tggcgcggct ggcgagactc
      241 gagctggagg aggaggagaa ggagcggatg acggcccagc tcgactcgat cctggggtac
      301 attgacaagc tgaacgagct ggacacaacc cacgtcgagc cgacgacgac cgtcatcccc
      361 atggtcagcg tgatgcgcga ggacgtggtc cggccgtccc tgagccagga cgaggccctc
      421 gccaatgcac cggaccggga ggacgtatac tttcgcgtgc cgcggatcat cgaggattga
      481 ccggaggaga gcggagaccg aagcgtggac ctgtttcgcc tgaccataca cgagttgcgc
      541 gacctgatcc agaaggggga gatctccccg gtccaggtgg tccacaacta cctcgtccgg
      601 atcggggagg tcgaagaccg ggtccaggcc ttcaacacgc tgaccccgcg gacggccctg
      661 gcgtttgccg ggaagtttgc gaagatgcag gcggagaaga aaccgttgcc ccccctggcg
      721 ggaattcccc tggcgatcaa ggacgtcatc tgcaccaagg gggtgacgac cacctgctct
      781 tccaagatcc tggaaagcta tatcccgccg tataacgcca gcgtggtcaa ccggctgaac
      841 gcctcggggg cgctcatgct gggcaagacc aacatggacg agtttgccat gggctcctcc
      901 acggagaact cggccagcaa gcagaccagg aacccctggg atctgggatg cgttcccgga
      961 ggctccagcg gcggctcggc tgcagccgtg gcggcggaca tgtgtgcggg ggcgctgggg
     1021 acggacacgg gcggctcgat ccgccagcct gggggcttct gcggcgtcgc cgcgctgaag
     1081 cccacgtatg gccgggtctc acggtatggt ctggtggcct tcgcctcgtc gctggaccag
     1141 atcggcccca tcaccaagga cgtcagggac gcggcgattc tcctcaaggc gatcgccggc
     1201 cacgatcccc tggactccac ctccgccgag cttccagtcg ccgactacga gacggccctc
     1261 accggggaca tccggggact gcgcatcggg ataccggcag agtacttcat tcccggcatg
     1321 gacccggagg tggaggcggc catccgcggg gccatccagg tcctggaggg gctgggcggg
     1381 atacaggaat cggtctcgct gccgcacacg ggctacgcgg tggcggcgta ctacctggtg
     1441 gccacggccg a
//
LOCUS       DBNGZC010000178         4720 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_797441, whole genome shotgun sequence.
ACCESSION   DBNGZC010000178 DBNGZC010000000
VERSION     DBNGZC010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4720)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_797441"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..2024
                     /locus_tag="AC16G0_03315"
     CDS             234..2024
                     /locus_tag="AC16G0_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR5098683.1"
                     /translation="MALINGGEVIAEYLVREEVPYVFGVCGHGNGGFLDALYARRGAI
                     QTISTRHEQTAGHMADAYFRVAHRPAATLTSCGPGSCNIPIALACAFMDSSAFLAITA
                     NVPTSQFNRGPFQETYRHYQADFPAVIKPYVKRSWQPSRVDMLPLALHQAFSTMLGGR
                     PGPVNLDVPFNVFKDQDDIEIPDPKAWRQGICSRSEGDPMVVSRILDLLLQAKRPLIL
                     LGHGVALSEAGEEVTAFARRLQIPVIFSPNAFGALDMTDPLALGFIGRNGTYAANEAA
                     RTCDVLLALGVRFDDRSSSSWIPGYSYNIPPTKLIHVDIDVQEIGRNYPVHLGMVADA
                     RTVLRQMLAGLDARGAAGPPGDRKAWLADTAKWRQDWEEFIRPGWTSDVMPMHPQRVV
                     EALRKVMPDDGIVIPDSGIHHNWIVQFWKARHPQTVLNTWGFSAMGFGVCGVLGAKLA
                     APDRPCVAVCGDGGFMMRPDVLCTAVEYDIPAVWLIWNNFAYGAIRDIQTGLLDKREI
                     ATSFIRQSTGEFYNPDFVALARACGAEGIRVERPTDLPGAIETAIKAHAPFVLDVHVN
                     RDIKPPAIGAWELPPLPYGEPVFGKRRVTG"
     gene            2148..3998
                     /locus_tag="AC16G0_03320"
     CDS             2148..3998
                     /locus_tag="AC16G0_03320"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR5098684.1"
                     /translation="MLGGYMGRVLRVDLTTRRMADETIPEATLRAYIGGSGLGARLLF
                     DETARETDPLGPENRLLFLTGPLTGVTIPTSGRHAVIAKSPLGLWGEADCGGTFGHEL
                     KRAGIDGILVEGQADRPVYLLISDGVASLEDGRELWGRDTFDTQDVIRARHGERFVVT
                     CIGPGGEKLAPIAAIMNDGRNGRPAARGGLGAVMGSKRLKAIAVAGTARPRVVHDDQL
                     RPLVKQMTLRIKERTGPLRNYGTAGGVLTSEAIGDLAIQNWRRGTWRAGAEKLSGEAM
                     AETILTGRYFCKACTIGCGREIEITDGPYAGVKGAGPEYETLGGLGSMCLVDDLPAVA
                     KANELCNRYGIDTISTGAIIAFAMEAFERGLLRRSDADGLDLTWGNADTMVRLVEKIG
                     KREGIGELLGNGVRFLARHLGGEASTFAVEVKGLELAYHDPRALSSLAAAYATYPRGA
                     CHRSYSHYLERNTLPELGFEKALDRLATERKGVAAAVRQDYGGLYNSLKLCQFIMRGV
                     SVQEVVDCLNHVTGWELDLAEFMRAGERASNLKRLYNLRLGMSRKDDTLPSRILNEAL
                     PDGGAANYLPNLEAMLDEYYTYRGWTQEGIPTRAKLEALGLGGDGASLGL"
     gene            4139..>4720
                     /locus_tag="AC16G0_03325"
     CDS             4139..>4720
                     /locus_tag="AC16G0_03325"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR5098685.1"
                     /translation="MKAIVKTAPGPGNLAYTDFKDPEAQPGHVVIEVRATGLCGTDLS
                     LYTWAESMVRQFIPTPPMVMGHEFAGVVAGVGAGVTGVKLGDRVTANPIMYCGQCYYC
                     KAGRHSICDNRPQLGLGLTGCFARFVAVRQQNVYPLPPEASFEAAAMSEVLCVALHTL
                     DRIPVGPGDTVAVVGPGPMGFLVLLAARAAGASR"
BASE COUNT          793 a         1507 c         1675 g          745 t
ORIGIN      
        1 ccccatgaaa gggaccatgg acaacaccag ggccatggat gatctcggct acacacccga
       61 gtacgatatc cggaagggtc tggcggagta tgccaggcat ctgcgctgtt atccggcttg
      121 acatgacctg gcgaaaccgg gtcgtgcgaa atcgaagctg agaatcgagt atgtatacga
      181 aggtgggccc agtcgctctt tcggactcca gaacaggctg aaggaggaag gaaatggcac
      241 tcatcaacgg cggcgaggtc atcgcggagt atctggtccg ggaggaagtg ccgtatgtgt
      301 tcggcgtctg tggacacggg aacggcggct ttctggatgc cttgtatgcc cgaaggggtg
      361 ccatccagac catctccacc cgccacgaac agacggcagg ccacatggcg gatgcctact
      421 tccgggtggc ccaccggccg gccgcgacgc tgacctcgtg cgggcccggc tcctgcaaca
      481 tccccatcgc cctggcgtgt gccttcatgg attcctcggc cttcctggcc atcaccgcca
      541 acgtgcccac ctcgcaattc aatcgcgggc ccttccagga aacctatcgc cactatcaag
      601 ccgacttccc ggcggtcatc aagccctacg tcaagcgcag ctggcagccg tcccgggtgg
      661 acatgctccc gctggcgctg caccaggcct tcagcaccat gctgggcggg cggcccgggc
      721 cggtcaatct ggatgtgccc ttcaacgtgt tcaaggacca ggacgacatc gagatcccgg
      781 acccgaaggc gtggcggcag gggatctgct cgcggagcga gggggatccg atggtggtct
      841 cccgcatcct ggatctgctg ctgcaggcca agcgcccgct aatcctcctc gggcacggcg
      901 tggccttgtc ggaagctggc gaggaggtga ctgccttcgc ccggaggctg cagatcccgg
      961 tgatcttctc cccgaatgcc tttggggcgc tggacatgac cgacccgctg gcgcttggct
     1021 tcatcgggcg caacgggacc tatgcggcga acgaggcggc ccgaacctgc gacgtactgc
     1081 tcgcgctggg cgtgcggttt gacgaccgat ccagcagctc ctggatcccc gggtattcct
     1141 acaacattcc tcccacgaag ctcatccatg tagacatcga cgtgcaggag atcggccgga
     1201 actacccggt ccacctcggg atggtggcgg acgcgcggac ggtcctgcgc cagatgctcg
     1261 ccgggctgga tgccaggggg gctgccggac caccgggaga ccggaaggcc tggctggcgg
     1321 acaccgccaa gtggcggcag gactgggagg agttcattcg ccccggctgg accagcgacg
     1381 tgatgcccat gcatcctcag cgggtggtgg aggcgctccg gaaggtcatg ccggatgacg
     1441 gcattgttat tccggactcc gggatccacc ataactggat cgtgcagttc tggaaggccc
     1501 gacatcccca aacggtgctg aacacctggg ggttctccgc catggggttc ggcgtctgcg
     1561 gcgtgctggg cgccaagctg gcggcgccgg accgcccgtg cgtggcggtt tgcggcgacg
     1621 ggggcttcat gatgcgcccg gatgtcctgt gcacggcggt ggaatacgac atcccggcgg
     1681 tctggctcat ctggaacaat tttgcctacg gggccatccg ggatatccag accggcctcc
     1741 tggacaagcg agagatcgcc accagcttca tccggcagtc caccggcgag ttctacaacc
     1801 ccgacttcgt ggccctggcc cgggcctgcg gcgccgaggg cattcgggtg gagcggccca
     1861 cggacctgcc gggggccatc gagaccgcga tcaaggccca tgcccccttt gtgctggacg
     1921 tgcatgtgaa ccgggacatc aagcccccgg ccatcggggc ctgggagctg ccgcccctgc
     1981 cctacggcga gccggtcttc ggcaagcgca gggtgaccgg ctgagcgtgg cataacggcg
     2041 gattgcgtgg accaacctag gatccttgtt gacggggcct gtgaccctgc cggcgaccgg
     2101 cgatcctggg acgggcaccg gcgcaggcac ccacgaggga gagcgacatg ctgggcggtt
     2161 acatgggcag ggtcctccga gtcgacctga cgacgcgacg gatggccgac gagacgatcc
     2221 ccgaggcgac gctccgggcg tacatcggcg gcagcgggct gggggctcgc ctcctcttcg
     2281 acgaaacggc ccgggagacc gatcccctgg ggccagagaa tcgcctcctg ttcctcaccg
     2341 ggcccctgac gggggtgacg attcccacct ccggacgcca cgcggtgatc gccaagtccc
     2401 ccctggggct ctggggggag gcggactgtg gcggcacctt cggccacgag ctcaagcggg
     2461 cggggatcga cgggattctc gtcgaaggcc aggccgatcg ccccgtctac ctgctgattt
     2521 ctgatggcgt cgcctcgctg gaggatggac gggagctgtg ggggcgggac acgttcgaca
     2581 cccaggacgt aatcagggcc cgccacgggg agcggtttgt ggtcacctgc atcggccccg
     2641 gcggggagaa gctggcgccc attgcggcga tcatgaatga cgggcggaac ggccgcccgg
     2701 cggcgcgggg cggcttggga gcggtgatgg gctccaaacg gctgaaggcc atcgccgtgg
     2761 cgggaacggc gcgcccccgc gtggtccacg acgaccagct ccggcccctg gtcaagcaga
     2821 tgacgctccg catcaaggag cggactggcc ccttgcggaa ctacgggacc gccggggggg
     2881 tcctgacgtc ggaggccatc ggcgatctcg ccatccagaa ctggaggcga ggcacctggc
     2941 gcgcgggggc ggaaaagctc tcgggggagg ccatggccga gacgatcctg acgggccgct
     3001 acttctgcaa ggcctgcacc atcggctgcg ggcgcgagat cgagatcacg gacggaccct
     3061 atgccggggt gaagggcgcc ggcccggagt acgagacctt gggcggtctg ggctccatgt
     3121 gcctggtgga cgatctcccg gcggtggcga aggccaacga gctatgcaac cgctatggga
     3181 tcgatacgat ctccaccggc gcgatcatcg ccttcgccat ggaggccttc gagcgggggc
     3241 tgctccgccg gagtgatgcc gacggcttgg atctcacctg gggcaatgcc gacaccatgg
     3301 tccggctcgt ggagaagatc gggaagcggg aaggcatcgg cgagctcctc gggaacggtg
     3361 tccgcttcct ggcgcggcac ttgggcgggg aggcgtccac gtttgcggtg gaagtcaagg
     3421 ggctggaact tgcctaccac gacccgcggg ccctctccag tctggcggcc gcctacgcca
     3481 cctacccccg gggggcctgc caccgaagct acagccatta cctggagcgg aatacgctgc
     3541 ccgagctggg cttcgagaag gccctggacc ggctcgccac ggagcggaag ggggtggcgg
     3601 cggccgtgag gcaggactac ggcgggctct acaactcgct caaactctgc cagttcatca
     3661 tgcggggcgt cagcgtgcag gaggtggtcg actgcctgaa ccatgtcacc ggctgggagc
     3721 tggacctggc ggaattcatg cgggccggcg agcgggcgtc caacctgaaa cggctctaca
     3781 acctccgcct ggggatgagc cggaaggacg acaccttgcc gtcccgaatt ctcaacgagg
     3841 cgctcccgga tggtggcgcg gccaactacc tgccgaacct ggaggcgatg ctggacgaat
     3901 actacaccta tcggggctgg acgcaggagg gcattcctac gcgggccaag ctcgaggcct
     3961 tgggcctcgg gggggacggg gcgagcctcg gcctctgagc agacggtgtg tcgagcaagg
     4021 gctggaatgc aggggagacc ccgccggacg ttggcctccg tgggtaggca ctgaggcgtg
     4081 gcctaagggc cgcggcccgg ggggcacgga ggatcaagtc gacaagaagg aggcaaggat
     4141 gaaagcgatc gtgaagacgg cacccggacc cgggaacctg gcctacacgg atttcaagga
     4201 ccccgaggca cagcctggcc acgtcgtgat cgaggtccgg gccacaggtc tctgcgggac
     4261 ggacctcagt ctctacacct gggccgagtc catggtgcgg cagttcattc cgacacctcc
     4321 catggtcatg gggcacgagt tcgcgggggt cgtcgcgggg gtgggggccg gggtgaccgg
     4381 cgtcaagctc ggagaccggg tgacggcgaa tcccatcatg tactgcgggc agtgttacta
     4441 ctgcaaagcc ggtcggcaca gcatctgcga caaccgcccc cagctggggc tgggcctgac
     4501 cggctgcttc gctcggtttg tcgccgtgcg tcagcagaat gtgtatcccc tgccgccgga
     4561 ggcatccttc gaggccgccg ccatgagcga ggtcctctgc gtagccctgc acaccctgga
     4621 ccggatcccg gtcggccccg gcgacacggt ggccgtggtc ggaccggggc ccatgggctt
     4681 cctggtcctg ctggcggcgc gggctgcggg ggcctcgcgg
//
LOCUS       DBNGZC010000179         4223 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2166275, whole genome shotgun sequence.
ACCESSION   DBNGZC010000179 DBNGZC010000000
VERSION     DBNGZC010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4223)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4223)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4223
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2166275"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="AC16G0_03330"
     CDS             <1..899
                     /locus_tag="AC16G0_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098686.1"
                     /translation="RLIRMLVALWSVVTVVFVVMRLSGDPVPLLLPPDAPIAEMERLR
                     HEMGLDRPIFVQYGVFVQQAIRGEFGRSIHFRTSAMQVAWERVPATLELAFASFLCAI
                     VIAIPAGIISAVKRGSTYDTIVMGVALIGQAAPTFYIGIMLILVCSLWLGLFPTSGRG
                     EWRQLVLPAVTLGAFAMASIARITRSAVLEILRADFIRTARAKGLPEFWTIAKHTMRN
                     AAIPIVTIVGLQLGNLLGGAVVTETIFSWPGIGRLAIQSIFNRDYPIVQSCVFMTAAM
                     FVVVNFLVDVAYGILDPRIRRQ"
     gene            865..1764
                     /locus_tag="AC16G0_03335"
     CDS             865..1764
                     /locus_tag="AC16G0_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014538206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098687.1"
                     /translation="MASSIHASVDSKPVAADQRGRWQKVPLFVWASAAFVLVVVIAAI
                     AAPLVSPYDPVQQSLRERLRAPAWFPEGGLRRHLLGTDQLGRDVLSRIIYGSRVSLVV
                     GFAAVIIGGAVGGCLGLMSGYVGGLVDEIIMLIVDAQLAFPFILLAIGIIAVLGPSFG
                     NLIIIVGLSGWVTYARLVRAQVLTIKEREYVIAIRGLGGSSWRILANHIVPNTLAPFL
                     VVTTLELARTILLESTLSFLGLGIQPPTPSWGGMLNEGRGYLDSAWWISAFPGLVLML
                     TALVVSRVGDWLRDVLDPTLRGV"
     gene            2076..3371
                     /locus_tag="AC16G0_03340"
     CDS             2076..3371
                     /locus_tag="AC16G0_03340"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5098688.1"
                     /translation="MKRTTLAWLMIGLLMQAGAAVAGELPPKPAKLTMAVVAGGETKG
                     LKAILPMWEKETGVPVELVEYPYSTLYEKLVTAFQANASTFDLLIADDPWMPKFGSEQ
                     WLVPLDSTFGYTRDDDIFPVVYDLGSWPPPRGPIPPGEQSKTRHLYAITLVGNVEFFM
                     YRKDLVPAPKTWSDVTANARKVQNPKTPLYGFVIRGAKGNPVISEFTPVLLSYGARIF
                     DDNWKVVLNSKEAVAALTFFAKTLKQYAPPGVENYDAAERAREVATGRAAQGFIWPAE
                     ITDIVENPSVSKVVGKMGYTLAPAGPGGKHAPLIGTWLLAIPKSSQNQRWAYEFITWA
                     TSARVQKPYALGGGIPFRKSVLLDPEMNKRFSFFKAMADSLAAPAEWRPRTQEWFAVE
                     AILGTHVNAALAGLETPEAAIAKAAGEIESHMKEAGYSR"
     gene            3466..>4223
                     /locus_tag="AC16G0_03345"
     CDS             3466..>4223
                     /locus_tag="AC16G0_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5098689.1"
                     /translation="MLAALVVYPLIYNIGLSLRNFRTGEFVGLHNFARAFQDPLFLAS
                     LRATAVYVVAALAIEVGLGLSLALLVHRTIKGALSRTLIYLLFIIPIVTPPVAAGVIA
                     RLIYTPTYGVLNVILLRLGLIHGDILWLSGAGTAMFAVISVDVWQWMPFVFLVCFAGL
                     QAVPLDAVEAASVDGAAGWHLFWFIELPYLRSLLLLVLIFRFTDTFRVFDHVLVLTGG
                     GPGASTEFLSLYLYRIAFKFFNLNYAAALSMYVL"
BASE COUNT          608 a         1400 c         1399 g          816 t
ORIGIN      
        1 atcggctgat tcgcatgctg gtcgccctct ggagcgtggt gacggtggtc ttcgtcgtca
       61 tgcggctctc gggagacccc gtgccgctcc tcctgccgcc ggacgcaccc atcgcagaga
      121 tggagcggct ccgtcacgag atgggactgg atcggccgat cttcgtccag tacggcgtgt
      181 tcgtgcagca ggccatccgg ggcgagttcg gccggtctat ccactttcgg acctcggcca
      241 tgcaggtggc ctgggagcgg gtgcccgcga ccctcgagtt ggcgttcgcc tccttcctgt
      301 gcgccatcgt gattgccatt ccggcgggaa tcatctcagc cgtcaagcga gggagcacgt
      361 acgacaccat tgtgatgggc gtggcgctga tcgggcaggc ggcgccgacc ttctacatcg
      421 ggatcatgct gatcctggtc tgctccctct ggctcggcct gttccctacg tctggccggg
      481 gggagtggcg gcagctcgtc ctgccggcgg tgaccctcgg ggcctttgcc atggccagca
      541 tcgcccggat cacccgctct gcggtgctgg agattctgcg cgccgacttc atccgcacgg
      601 cgcgcgccaa ggggctcccc gagttctgga ccatcgccaa gcacaccatg cggaacgccg
      661 cgatccccat cgtcaccatc gtgggcctgc agctcgggaa cctgctcggg ggggcggtgg
      721 tgacggagac gatcttttcg tggccgggca tcggccgcct ggcgatccag tcgatcttca
      781 atcgagatta tccgatcgtg cagtcgtgtg tcttcatgac ggcggcgatg ttcgtcgtgg
      841 tgaacttcct ggtggatgtg gcgtatggca tcctcgatcc acgcatccgt cgacagtaag
      901 ccggttgccg cagaccagcg tggccggtgg cagaaggtgc cgctcttcgt ctgggcctcc
      961 gcggccttcg tgctcgtggt ggtgatcgct gccatcgcgg cgcccctggt cagcccctac
     1021 gacccggtcc agcagtcact ccgcgagcgg ctcagggcgc ccgcgtggtt ccccgagggg
     1081 gggcttcgcc gacacctgct cggaaccgat cagcttggcc gggatgtgct gagccggatc
     1141 atctacggct cgcgggtctc gctggtggtc ggtttcgcgg cggtcatcat cggcggggcg
     1201 gtcggcggat gcttgggact gatgtcgggc tacgtcggcg ggctggtcga cgagatcatc
     1261 atgctgatcg tggatgcgca gttggcattt ccattcatcc tgctggcgat cgggatcatc
     1321 gcggtgctgg ggccgagctt cggcaacctg atcatcatcg tggggctgag cggctgggtg
     1381 acctacgccc gcctggtccg cgcccaggtg ctgacgatca aggagcgtga gtatgtgatc
     1441 gccatccgcg ggttgggcgg gtccagctgg cgaatcctcg ccaatcacat cgtgcccaac
     1501 acgctggctc cgttcctggt ggtcacgacg ctggagctgg cccgcaccat cctgctggag
     1561 tccaccctca gcttcctcgg cctgggcatc cagcctccga ccccgagctg gggcggcatg
     1621 ctgaacgagg gacgcggtta cctcgattcc gcttggtgga tctcggcttt tccggggctc
     1681 gtcctgatgc tgaccgcgct ggtggtgagc cgggtcggcg actggctccg ggacgtcctg
     1741 gaccccaccc tccggggcgt gtaggacggg gcttcaccct cctcggcggc acaggctctc
     1801 ggcccaccgg ttttgtcttg caccctcaga gctaggccgg tagcattttt tcgtgcggtc
     1861 gtgggtgacg gcggcaatct cctgatgatc ggccgtatgg cggaccagtc gcccgggcgc
     1921 ccgcgcctcg acagggggga gctgggccgg agcacgcggg ctccgggctg gaccgcaggc
     1981 tggatgctct gcggctgcgt gcgccaaagg gggtgagatg aacctcaggt gagagcaacg
     2041 tccgggggag gtttcaatcg acagggaggg agcccatgaa acggacgacg ctagcctggc
     2101 tgatgatcgg cctgctgatg caggctggag cggcagttgc gggtgagctg ccgcccaagc
     2161 ccgcgaagct caccatggcg gtggtggcgg ggggcgagac caaggggctc aaggccattc
     2221 tgccgatgtg ggagaaggag accggggttc cggtcgagtt ggtggagtac ccgtattcga
     2281 ccctgtacga aaagctggtg acggccttcc aggccaatgc ttccaccttt gacctgctca
     2341 tcgccgacga cccctggatg ccaaagttcg gcagcgagca atggctggtc cccctcgact
     2401 cgacgttcgg gtacacgcgc gacgacgata tcttccccgt ggtgtacgac ctgggttcct
     2461 ggccgccccc gcgcgggccc atcccacccg gagagcagag caagacccgg cacctgtacg
     2521 ccatcaccct ggtggggaac gtcgagttct tcatgtaccg caaggacctg gtcccggcgc
     2581 cgaagacctg gagtgacgtc accgccaatg ccaggaaagt ccagaacccg aaaacgcccc
     2641 tctacggttt tgtgattcgc ggcgccaagg gaaacccggt gatctcggag ttcacgccgg
     2701 tgctcctgtc ttacggcgcc cgtatcttcg atgacaactg gaaggtcgtc ctcaactcca
     2761 aggaggcggt tgcggccctg acgttcttcg ccaagaccct gaagcagtac gcaccgcctg
     2821 gcgtggagaa ctacgatgcg gccgagcgcg cccgcgaggt ggccacgggc cgggccgccc
     2881 agggcttcat ctggccggcc gagatcaccg atatcgtcga gaacccctcg gtgtccaagg
     2941 tggtcgggaa gatgggatac accctggcgc cggccggccc cggcggcaag cacgcccccc
     3001 tcatcggcac ctggctcctg gccatcccca agagctccca gaaccagcgc tgggcctacg
     3061 agttcatcac ctgggcgacc agcgcccggg tccagaagcc ctacgccctg ggcgggggca
     3121 tccccttccg caaatccgtc ctgctggacc cggagatgaa caaacggttc tccttcttca
     3181 aggccatggc cgattcgctg gcggccccgg cggagtggcg cccgcggacc caggagtggt
     3241 tcgcggtcga ggccatcttg gggacgcacg tgaacgcggc gctggccgga ctggagaccc
     3301 ccgaggccgc catcgccaag gctgccggtg aaattgaaag ccacatgaaa gaggcgggct
     3361 attcccgcta aggccggggc ccggggtccg ggcggccccg ggctcgcctc cgcccggtta
     3421 cgccggcaac tggcgccgta tctgctcctg gcacctccgg tcctgttgct ggccgccctg
     3481 gtcgtctacc cgctgatcta caacatcgga ttgagtctcc ggaacttccg gaccggcgaa
     3541 ttcgtcggcc tgcacaattt cgcccgcgcc ttccaggacc ccctcttcct agcgtcgctc
     3601 cgggccacgg cggtgtacgt cgtggcggcg ctcgccatcg aggtggggct gggtctctcc
     3661 ctggccttgc tggtccatcg gacgatcaag ggcgccctgt cccggacgct gatttacctc
     3721 ttgttcatca tccccatcgt gacgccgcct gtggcggcag gggtcatcgc ccggctcatc
     3781 tataccccga cgtacggcgt gctcaacgtc atcctgctcc ggctggggct gatccacggg
     3841 gacatcctct ggctctcggg ggccgggacg gcgatgttcg ccgtgatcag cgtggacgtc
     3901 tggcagtgga tgcccttcgt cttccttgtc tgcttcgccg gcctccaggc cgtccccctg
     3961 gacgccgtag aggcggccag cgtggatggg gcggccggct ggcatctctt ttggttcatc
     4021 gaactgccct acctgcggtc gctcctcctg ctggtgctca tcttccggtt caccgatacc
     4081 ttccgggtct ttgaccacgt tctggtcctg accggcggcg gcccgggggc ctccacggag
     4141 tttctcagct tgtatctcta ccgcatcgcc ttcaagttct tcaatctgaa ctacgccgcg
     4201 gcgctctcca tgtacgtcct gat
//
LOCUS       DBNGZC010000180         2473 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_15243, whole genome shotgun sequence.
ACCESSION   DBNGZC010000180 DBNGZC010000000
VERSION     DBNGZC010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_15243"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /locus_tag="AC16G0_03350"
     CDS             complement(<1..1137)
                     /locus_tag="AC16G0_03350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HIR5098690.1"
                     /translation="MAYTQEGRLIALDTPLGQDVLQLQGLTGHEGISRLFRFDLELFS
                     EDPAISYDKIVGKNVTVTLVLEDGDERYFNGFVSRFVQSGQDERFTHYRAEMVPWLWY
                     LTRTADCRIFQNKTIPDIITQIFTDLGFKDYKNVLQGNFEPREYCVQYRETDFNFVSR
                     LMEQYGIFYFFEHENGKHTLVLANSPTAHQPCPGQPKARYDYTATGIEREDVIRDWHV
                     EYELRPGKYALTDYNFETPSSSLAVNEATVVQVSGNTKYEIYDYPGEYLKKPQGQNLA
                     KIRMEEEETPATVVSGASLCRAFASGYRFDLTEHYRQDLNKAYVLTGVQHTASVGECY
                     TTGDDSGETYSNEFTCIPHSVPYRPARNTPRPVVQGSQTAMVAGK"
     gene            complement(1267..>2473)
                     /locus_tag="AC16G0_03355"
     CDS             complement(1267..>2473)
                     /locus_tag="AC16G0_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5098691.1"
                     /translation="EGRLIGELRTIRGKLEAHAAATRGGGAEKTNGKLSPDEVAALQA
                     DLTRLSDELAKAQGEDPLMQVCVDGQTIAEVVSGWTGIPVGKMVKDEIKTVLSLKDFM
                     EKRIIGQTHALEAISQRIRTARAKLEDPRRPTAVFLLVGPSGVGKTETALTLADVLFG
                     GERNLVTINMSEYQEAHTVSGLKGSPPGYVGYGEGGVLTEAVRRKPYCVLLLDEIEKA
                     HTDVMELFYQVFDKGVLEDAEGREIDFKNTVILLTSNVGTDTIMKLCADPETRPEPVA
                     LVEALRPDLLKVFKPAFLGRMIVVPYYPIADDAMRKIIELQLGRIRTRLLENNRTQFT
                     YDEALVSAIARRCTEVESGARNVDHILTRTLLPEISQEFLARMAAGEAISKVHVSVDG
                     GGAFQYAIT"
BASE COUNT          402 a          806 c          780 g          485 t
ORIGIN      
        1 cttccctgcc accattgctg tctgcgagcc ctggacgacc ggccggggcg tgttgcgcgc
       61 cggacggtac ggaacggaat gggggatgca cgtgaactcg ttagagtagg tctccccgga
      121 gtcgtccccg gtcgtgtagc attcgccgac ggaggcggtg tgctgcaccc cggtcagcac
      181 ataggcctta ttcaagtcct ggcgatagtg ctccgtcagg tcgaaccggt agcccgaggc
      241 gaatgcgcgg cacaggctgg ccccgctcac cacggtcgca ggcgtctcct cctcctccat
      301 gcggatcttc gcgagattct gcccctgggg ttttttcagg tactcccccg gatagtcgta
      361 gatctcgtac ttggtgttcc cgcttacctg gacgaccgtg gcctcgttca cggccaggct
      421 gctactcggc gtttcgaagt tatagtccgt cagcgcgtac ttgccgggcc gcaactcgta
      481 ttccacgtgc cagtcccgga tcacatcctc tcgctcgatg ccggtggcgg tgtagtcgta
      541 cctggctttg ggctgaccgg ggcacggctg gtgagcggtc ggggagttgg ccagcaccag
      601 cgtgtgtttc ccgttctcgt gctcgaagaa atagaagatg ccgtactgct ccatgagccg
      661 cgacacgaag ttgaaatccg tctcccgata ctggacgcag tactcgcgcg gctcgaagtt
      721 tccctggagc acgttcttgt agtccttgaa gccaaggtcg gtgaagatct gggtgatgat
      781 gtcggggatc gtcttgttct gaaagatgcg gcagtctgcc gttcgcgtca ggtaccagag
      841 ccagggaacc atctcggccc ggtagtgagt aaaccgctcg tcctggccgc tctggacaaa
      901 acggctcacg aagccattga agtaccgctc gtcaccgtcc tccaggacca gggtgacggt
      961 gacgttcttg cccacgatct tgtcatacga gatcgcgggg tcttccgaga aaagttccag
     1021 gtcgaacctg aaaagccgag agattccctc gtggcccgtg aggccttgca gctgcaagac
     1081 atcctggccc aggggcgtat cgagcgcgat caggcgtcct tcttgcgtgt aggccatttt
     1141 gacgacgcct cctgtaccgc ttcaccccag gacatgagga tcggccaacg tgagaagagt
     1201 tggcaccgcg agacccggta acagccgtcg tagatacgct cgcctgacgt gaggagcggc
     1261 tgcatcctaa gtaatcgcat actggaaggc tccgccgccg tccaccgaga cgtgcacctt
     1321 ggagatcgcc tccccggcgg ccatccgcgc caggaactcc tgcgagatct caggcaggag
     1381 cgtccgggtg aggatgtggt ccacgttcct ggccccgctc tcgacctcgg tgcagcggcg
     1441 ggcgatcgcc gagaccaggg cctcgtcgta ggtgaactgg gtccggttgt tctccaggag
     1501 gcgcgtgcgg atccggccga gctgcaactc gatgatcttc cgcatggcgt cgtccgcgat
     1561 cgggtagtag ggcaccacga tcatgcggcc caggaaggcc ggcttgaaga ccttgagcag
     1621 atcggggcgg agcgcctcca ccagcgccac cggctcgggc cgggtctccg ggtcggcgca
     1681 gagcttcatg atggtgtcgg tccccacgtt ggaggtcagc aggatcaccg tgttcttgaa
     1741 gtcgatttcc cgcccctcgg catcctccag gacacccttg tcgaacacct ggtagaacag
     1801 ctccatcacg tcggtgtgcg ccttctcgat ctcgtcaagc aggagcacgc agtacggctt
     1861 gcgccgaacc gcctcggtga gcaccccgcc ttcgccatac cccacgtagc cgggcggcga
     1921 gcccttcagg ccggagacgg tgtgcgcctc ctggtactcc gacatgttga tggtgaccaa
     1981 gttccgctcg ccgccgaaaa ggacgtcggc cagcgtgagg gcggtctcgg tcttgccaac
     2041 gccgctcggg cccaccagca agaacaccgc cgtggggcgg cgcgggtcct ccagcttcgc
     2101 ccgcgcggtc cggatgcgct ggctgatggc ctccagggcg tgggtctgcc cgatgatgcg
     2161 cttctccatg aaatccttga gcgacaggac cgtcttgatc tcgtccttga ccatcttgcc
     2221 gacggggata cccgtccagc cggagaccac ctcggcgatg gtctgcccgt ccacgcacac
     2281 ctgcatgagc gggtcttcgc cctgggcctt cgccagctcg tcgctgagac gcgtgagatc
     2341 cgcctgaagc gccgcgacct catcggggga cagcttgccg ttcgtctttt cggcgccccc
     2401 gccgcgggtg gccgcggcgt gcgcctccag cttcccgcgg atggtccgca gctcgccgat
     2461 cagccggccc tcc
//
LOCUS       DBNGZC010000181         1784 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2460974, whole genome shotgun sequence.
ACCESSION   DBNGZC010000181 DBNGZC010000000
VERSION     DBNGZC010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1784)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1784)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1784
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2460974"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="AC16G0_03360"
     CDS             <1..675
                     /locus_tag="AC16G0_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098692.1"
                     /translation="TALELTHTGKKNLVVMTVRIARGPDAGYQDLDVKRLFSAYEQQL
                     FTRVVALAEKAGKHVDLLVVPSSDVFQAAAITAAQLDSHEIYAGRSGVMTPEEQARRI
                     GHAWDNLPEAPKHTLRYRVVEPDGQIRDFYLGAHDPNLTAREIDLIHHLWLDVTEIGG
                     AEGVHHRDVVALALSCLAEELQGPTRGVLLGKLTMGAANTQATLPTRDQQDHLRGSGV
                     SMPAPI"
     gene            complement(725..1474)
                     /locus_tag="AC16G0_03365"
     CDS             complement(725..1474)
                     /locus_tag="AC16G0_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020565213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5098693.1"
                     /translation="MFESSPGWQASRTVDPTAAHEESVVIVIEDDPQIRHFLRTTLPN
                     HGYRLHEATTGAEGLREAEARAPDIIILDLGLPDLDGLVVIRRLRGWTSVPIVVLSAR
                     GQERDKVAALDAGADDYVSKPFGIGELLARMRVALRHAAHGAEREAGSPCTVGDLHVD
                     LAHRRVTMAGKEIHLTPIELRLLALLAKHAGKVLTQRQLLTEVWGPPYADQAHYLRVY
                     MAQLRRKVEADPARPRYLLTEAGVGYRLAAD"
     gene            complement(1467..>1784)
                     /locus_tag="AC16G0_03370"
     CDS             complement(1467..>1784)
                     /locus_tag="AC16G0_03370"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5098694.1"
                     /translation="DNALKYTPAGSPIEVSASQEDSTVRIEVVDRGPGIPPGDGQRVF
                     EKFYRGQTGTIRGVGLGLAICRAIVEAHGGTIWAENRPGGGGVFRFTLPAKEAPPEVP
                     RDV"
BASE COUNT          306 a          591 c          568 g          319 t
ORIGIN      
        1 actgccttgg agctgacgca cacggggaag aagaacctcg tggttatgac tgtgcggatc
       61 gcgcggggac cggacgcagg ataccaggat ctggatgtga agcggttgtt ctcagcctac
      121 gaacagcagc tattcacccg cgtggtggcc ttggcagaga aagccgggaa gcacgttgat
      181 ctgcttgtcg tgccctcctc agacgtgttc caggcggctg cgatcacggc tgcgcaactg
      241 gattcgcatg agatttacgc cgggcgctcg ggcgtgatga cgccggagga gcaggcccgg
      301 cggatcggcc acgcctggga caacctgcca gaagcaccga agcacaccct gcgctaccgc
      361 gtggtggagc ccgatggcca gattcgcgat ttttacctgg gcgcgcatga cccgaacctg
      421 accgccaggg aaatcgactt gattcaccac ctctggctgg atgtgaccga aatcgggggc
      481 gcggaaggcg tgcaccatcg ggatgtggtc gccctggcgc tgtcttgtct ggcggaggag
      541 ttgcagggtc caacgcgggg tgtgctcttg ggcaagctca ccatgggggc cgcgaacacg
      601 caggccaccc tcccgacacg agaccagcag gaccatctcc gcggatcggg cgtctcgatg
      661 cctgccccca tttgaagaga ccggacggtg ttggggttcc ccccttcttc gaactcttag
      721 ccgatcaatc cgccgccagg cggtacccca ccccagcttc ggtaagcaga tacctgggtc
      781 gcgccggatc cgcctccacc ttgcggcgaa gctgcgccat atagacccgc aggtagtggg
      841 cttggtctgc gtagggcggg ccccacacct cggtcaggag ctggcgttgc gtgagaacct
      901 tccccgcatg tttcgccaac aaggcgagca ggcgtaactc gatgggggtc aggtgaatct
      961 ccttgcctgc catcgtcacc cgccggtgtg cgaggtcgac gtggagatcc ccgaccgtgc
     1021 agggtgagcc ggcttcccgc tccgccccat gcgcggcgtg ccgcaaggcc acgcgcatcc
     1081 gcgccagcag ttccccgatg ccgaacggct tgctcacata gtcgtcggcg ccggcatcca
     1141 gcgcagccac cttgtcgcgc tcctgcccgc gggccgaaag gacgacgata ggcacgctgg
     1201 tccagccgcg aagacgccgg atcaccacca gcccgtcgag gtcgggaagt ccgaggtcga
     1261 gaatgatgat gtcgggggca cgggcttcgg cctctcgcag tccctccgca ccggtcgtcg
     1321 cttcgtgaag ccgatatccg tggtttggca acgtcgtccg aaggaagtga cggatctgag
     1381 ggtcgtcctc gataacgatg acaaccgact cttcatgcgc tgcagtggga tctacggttc
     1441 gcgacgcttg ccatccgggg ctggactcaa acatctcggg gcacctccgg tggtgcctct
     1501 ttggccggca atgtgaatcg gaataccccg ccccctcccg gccggttttc ggcccagatc
     1561 gtccccccgt gggcctcgac gatggcccgg cagatggcca gccctaaccc aaccccccgg
     1621 atggtcccgg tttgcccccg gtagaacttc tcgaacactc gctgcccgtc gccaggcgga
     1681 atccctgggc cccgatcgac cacctcgatt cgcacggtgc tgtcctcttg cgaggcagac
     1741 acctcgatcg gacttcccgc cggcgtgtac ttgagggcat tgtc
//
LOCUS       DBNGZC010000182         3157 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1971424, whole genome shotgun sequence.
ACCESSION   DBNGZC010000182 DBNGZC010000000
VERSION     DBNGZC010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1971424"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /gene="moaA"
                     /locus_tag="AC16G0_03375"
     CDS             <1..817
                     /gene="moaA"
                     /locus_tag="AC16G0_03375"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR5098695.1"
                     /translation="LGIRTIRLTGGEPLMRQDIDILIAGLTRLEPELDLSMTTNGFFL
                     KEKAQRLAEAGLKRINVSLDSLQPERFERMVLRDGQLVSKILDGMRIASEVGLSPIKI
                     NCVVMRGHNDDELVAFARLGREHGYQVRFIEYMPLDAQGAWNMQAVVPGAEILARIHA
                     VFPLAPVTGNGSEPATVYRFADGRGSIGVIASVTEPFCDQCNRIRITADGQLRTCLFS
                     ISEHNLKGLLRSGATDEEIADFILAAVWKKEPGHKINQPDFVKPTRSMSAIGG"
     gene            916..1887
                     /locus_tag="AC16G0_03380"
     CDS             916..1887
                     /locus_tag="AC16G0_03380"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR5098696.1"
                     /translation="MNASGLTREQVLEIYSCLRLTRSLEERIGILYRQGKIFGGVYLS
                     TGQEAVSVGGAYALEPGDIIVPSHRDMGAHLLRGIKPREILAQYLGRVGGLSRGRDGN
                     VHFGDIQRGDVAFISPMADGIPVAAGVGLALRRQGKGRVAMTWFGEGAASRGDFHEGI
                     NLAAVLQAPVVFLCNNNQFAYSTPLQKQTRVTDIANRAEGYGIPGVIVDGNDVLEVYA
                     AAREAVARARRGEGPSLIECKTMRMRGHSEHDDASYVPRELLEEWRGKDPIQRLEARL
                     REWGVLDPETERQIGKRIADELEAAVAWAEASPFPDGKEVTVGVYST"
     gene            2121..3095
                     /locus_tag="AC16G0_03385"
     CDS             2121..3095
                     /locus_tag="AC16G0_03385"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR5098697.1"
                     /translation="MGDITYIEAIRQALWEEMERDERVFLIGEDIGHYGGAFKITLGF
                     LEKFGADRVIDTPIAESAIVGAATGAALMGLRPVVEMQFADFLACGFDQVVNMAAKFH
                     YRTGTSVPLVIRCPSGGGVHGGPFHSQNPEAWFVHVPGLKVVAPSTPYDAKGLLKAAI
                     RDPDPVIYFEHKYLYRRIKGKVPDEDFSVPIGKAEVKREGTDVSVISYGAMVHQAFEA
                     ANRVGERGISVEILDLRTLVPLDQDAILATARKTGKVVIVHEATRTGGVGGEVAARIS
                     ESAFDRLDGPILRVTAPDTPVPFTTTLEEDFLPNAERIVLAIERLAAY"
BASE COUNT          561 a          977 c         1090 g          529 t
ORIGIN      
        1 cctgggtatc cggaccatcc gcttgaccgg gggcgagccc ttgatgcgcc aggacatcga
       61 catcctgatc gcggggctga cgcgtctcga accggagctg gatctctcca tgacgaccaa
      121 cggattcttt ttgaaggaga aggctcaacg actggcagag gctggtctca agcggatcaa
      181 cgtcagtctg gattcactcc agcccgagcg cttcgagcgc atggtgctcc gcgacggcca
      241 actggtttcc aagattttgg acgggatgcg catcgcgagc gaggtcgggc tctcccccat
      301 caagatcaac tgcgtggtca tgcgcggtca caacgatgac gagctggtgg cctttgcgcg
      361 gctggggagg gagcacgggt accaggtccg cttcatcgag tacatgcccc tggatgccca
      421 gggcgcctgg aacatgcagg ccgtcgtgcc cggggccgag atcctggcgc gcatccacgc
      481 ggtgttccct ctggccccgg tcacgggcaa cgggagcgag cccgccacgg tctatcgttt
      541 cgccgacggg cgcggctcca tcggggtcat cgcctcggtc accgagccgt tctgcgacca
      601 gtgcaaccgc atccgcatca cggcggacgg ccagctccgc acgtgtctct tctcgatctc
      661 ggaacacaac ctgaagggtc tcctgcgatc cggcgccacc gacgaggaga ttgcggactt
      721 catcctcgcc gccgtctgga agaaggaacc gggccacaag atcaaccagc cggatttcgt
      781 caagccgact cgttccatgt ccgccatcgg cggctgacca ggcctgccgt ccccactcgt
      841 ggcaggcctc gtgaggagtg agagccccgg gtgggtgacg gcgcgtagga gagcggaaat
      901 caaggaggga cgctcgtgaa cgcgtcgggt ttgacccgcg agcaagtcct cgagatttac
      961 tcctgcctgc gactcacccg gtccctcgaa gaacgcatcg gaatcctgta tcgccagggc
     1021 aagatcttcg gcggggtcta cctgtccact gggcaggagg ctgtgtccgt cggaggggcc
     1081 tacgccttgg agcccggaga catcattgtc ccctcccacc gagacatggg ggcccatctc
     1141 ctgcggggga tcaagccccg cgagattctg gcgcagtacc tgggccgggt cggggggctg
     1201 agtcgcgggc gggatgggaa cgtccatttc ggggacatcc agcgagggga cgtggccttc
     1261 atcagcccga tggcagacgg gatccccgtg gctgcgggcg tggggctggc gctgcggcgc
     1321 caaggcaaag ggcgggtggc gatgacctgg ttcggcgagg gggccgcaag ccggggggac
     1381 ttccacgagg gcatcaactt ggcggcggtg ctccaggctc ccgtcgtatt tctgtgcaac
     1441 aacaaccaat ttgcgtactc gacgccgctc cagaagcaga cgcgggtgac cgacatcgcg
     1501 aaccgggcgg aaggctacgg cattccaggt gtcatcgtgg acggcaacga cgtcctggag
     1561 gtctacgcag cggcccgtga ggccgtggcc cgggcccggc ggggggaagg gccgagcctg
     1621 atcgagtgca agaccatgcg aatgcggggg cactcggagc acgacgacgc ctcctacgtt
     1681 cctcgggagc tgctggagga atggcgcggg aaggatccga ttcagcgcct cgaggcgcgc
     1741 ctccgggagt ggggggtgct ggacccggaa acggagaggc agatcggcaa gcgcatcgcg
     1801 gacgaactcg aggcggccgt ggcctgggcc gaggcgagcc cgttcccgga cggcaaggaa
     1861 gtgacggtgg gggtctattc gacctaaaag gctgctgaaa aaggcccatc tgcggcgttg
     1921 gcgcgctcgg gctctcgcgg cggcgtacgc ctaagtacgc ttcactcggc cctgcgcgcg
     1981 ccgccttgca tctggacctt tttgagcagc ctgggcgaaa acgagttttt cagcatcctg
     2041 ctaatgtgag ccggcgttca ggcgaaaggg cacggcgggg ggggacaccg ccccgctgac
     2101 gcggcggagc aggctctcgt atgggagaca tcacgtatat cgaggccatc cggcaggcgc
     2161 tctgggagga gatggagcga gacgagcggg tgttcctcat cggggaggac atcggccact
     2221 acggcggggc cttcaagatt accctcggct tcctagagaa gtttggcgcg gatcgggtga
     2281 tcgacacgcc catcgccgaa tccgccatcg tgggcgccgc caccggggcg gccctcatgg
     2341 gattgcgccc ggtggtggag atgcagttcg ccgacttcct ggcctgcggg ttcgatcagg
     2401 tcgtcaacat ggccgccaag tttcactacc ggacggggac cagcgttccc ctggtcatcc
     2461 ggtgtccctc cggcggcggc gtgcacgggg gcccattcca ttcgcagaat cccgaggcat
     2521 ggttcgtcca cgtgccgggc ctgaaagtgg tagccccctc gaccccgtat gacgccaaag
     2581 gactgctcaa ggcggcaatc cgcgacccag atcccgtgat ctacttcgaa cacaagtatc
     2641 tctatcgccg gatcaagggg aaagtcccag acgaggattt cagtgttccc atcggcaagg
     2701 ccgaggtgaa acgcgagggc acggacgtca gcgtcatcag ttatggtgcc atggtgcatc
     2761 aggccttcga ggcggccaat cgcgtgggcg agcgggggat ctcggtggag atcctggatc
     2821 tgcggacgct cgtgccgctg gaccaggacg ccatactggc gactgcgcga aagaccggca
     2881 aggtggtcat cgttcacgag gccacccgga cggggggcgt cggaggcgag gtggctgctc
     2941 gcatctcaga gtctgccttc gaccgactcg acggtccgat ccttcgtgtc acggcgccgg
     3001 ataccccggt gccgttcacc acaacgctgg aagaagactt cctgccaaac gccgagcgca
     3061 tcgtgctggc catcgagcgg ctggccgcat actgactgaa cgtgtgacgc tccgcggctt
     3121 catcggccgt gcagatgaat gcggcgagcg tcgaacc
//
LOCUS       DBNGZC010000183         3454 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_309128, whole genome shotgun sequence.
ACCESSION   DBNGZC010000183 DBNGZC010000000
VERSION     DBNGZC010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_309128"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="AC16G0_03390"
     CDS             complement(<1..609)
                     /locus_tag="AC16G0_03390"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5098698.1"
                     /translation="MKPPGVHSFRAAFSTANAASFLVNTYGSFFLLLPVYLHQTGSSP
                     AEIGLVAGLLRASSLLARPLGGRLLDRFGRRQMISAGAALTILAVLSLFVFPRVGFPF
                     MLMRVLQGVGTSLIDSGFGAVVADLSPPGARAQVFAIYTVWINLAGAVMPGAGEAIVR
                     RAGFPPLFGAAALALAASLGLVWRLPQTTRPRAEQTGSLRGVM"
     gene            complement(748..1614)
                     /locus_tag="AC16G0_03395"
     CDS             complement(748..1614)
                     /locus_tag="AC16G0_03395"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5098699.1"
                     /translation="MHETGGRTLNPVIDSHLHVWDESAAGKDPGPMRVGYSPQASASV
                     ELFMDYMDEAGVEKAVFVQPWFYHWDNSYLAACIGRDPDRFRGVCVIDPRGPAAPAEL
                     KKWRNRGVTGIRLRPLREGENPAPGSWFGTDETMPLWETIAETGTIVCVLHADTDLAR
                     LPGLLKRFPSVKVVIDHLNNPIPGEGLRQPRFQAYLELASLPTVYVKLSGFHHWCRER
                     YPYRDGMPYVEAAVNAFGADRCMWGSDYPHVLAGCGYVRNRNYLSREAGFLSRRQLDA
                     IMGGTAERLWFG"
     gene            complement(1587..2324)
                     /locus_tag="AC16G0_03400"
     CDS             complement(1587..2324)
                     /locus_tag="AC16G0_03400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5098700.1"
                     /translation="MDRKPWSPHGRALLDYFNGRTSAEVVVHEADGETEVVPARVFFR
                     EAEEFSALEEAALDLCRGRVLYVGAGAGCHSLVLQERRLHVCAIDVAPEAVEVMRKRG
                     VRDVRCADVFGFRAEPFDTLLMLMNGIGVVGSLAGLQRFLRDVKRLLRPGGQILLDSY
                     DPGPGAAQGVAAQGGPSGTGGYPGEMRFQLEYEGRMGPTLEWLFVDFETLAARAVREG
                     WSAEIIWREEEGHYLARLACTRREAGP"
     gene            complement(2409..>3454)
                     /locus_tag="AC16G0_03405"
     CDS             complement(2409..>3454)
                     /locus_tag="AC16G0_03405"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR5098701.1"
                     /translation="HLWDEPDEMKALFYPAWDRLEIREVAEPTPKPGEVIVKVEAVGI
                     CGSELHGYVTHAARRTPPLIMGHEFCGTVARNGAGVTDYRPGDRVAVNSVIACGRCEE
                     CLDGRSHLCREGEVYGTKRSGAFAELCAAPASTLLPLPANVSFLQAALTEPLANAVHA
                     LSQIRLRFPETLLIFGAGTIGLFVLQVARTAGVFRVAMADVSDARLEVARRLGADITV
                     NPRKEDILAASRDFTHGRGIDVAVDAVGVAETRHAAVMATRPGGDIIWLGLHDDPTQV
                     SGFAVVLGERRISGSFAVTPRDLRTAIGLFATGKIELTPWVRTFPLTEGARVFQELVT
                     APPQDYIKAILLP"
BASE COUNT          614 a         1226 c         1089 g          525 t
ORIGIN      
        1 catgacgcct cggagcgacc cggtttgctc cgcccgcggt cgggtcgttt gcggtagccg
       61 ccataccagg ccgagactcg cggccagggc cagcgccgct gccccgaaga gcggggggaa
      121 gccagcccgt cgcacgatcg cctctcccgc acccggcatc accgcccccg ccagattgat
      181 ccacacggtg tagatggcga acacctgggc cctggcaccg gggggactga gatcggccac
      241 caccgccccg aatccggagt cgatgaggga ggtccccacc ccttggagca cccgcatgag
      301 catgaagggg aagcctaccc gggggaacac gaagagcgaa agcaccgcca ggatggtcag
      361 ggctgcaccg gccgagatca tctggcgccg gccgaaccgg tccagcagcc ggccgcccag
      421 cggtcgggcc agcagggaac tcgcccggag gagacctgcc acgaggccga tctccgcggg
      481 agaactgccg gtctgatgca ggtagaccgg caggaggagg aagaacgagc cgtaggtgtt
      541 gaccaggaag ctggccgcgt tggcggtgct gaaggcagcc cggaagctgt gcacgccggg
      601 aggtttcatg gccccgcgac caacgccagc ctcaccccat caccttccct aggctgcttt
      661 cccacggaat tgcatcctcc ctccatccag cttccattca ggacctcgac accatggatc
      721 ggcgcatcca gtggagtgcc cggaccgcta tccgaaccag agccgctcgg cggttccccc
      781 catgatcgca tccagttgac gcctggagag gaagcccgcc tcccgcgaca ggtagttccg
      841 attgcgcacg tacccgcagc ctgccagcac gtgaggatag tcagagcccc acatgcaccg
      901 gtccgccccg aaggcgttga ccgccgcctc gacgtagggc atcccgtccc gatacggata
      961 gcgctccctg caccagtgat ggaatccgga gagcttcacg tagaccgtgg gaaggctcgc
     1021 caattccagg tacgcctgga atcgcggctg gcgcaggccc tcgcccggaa tagggttgtt
     1081 caggtggtcg atcacaacct tgaccgaggg gaaccgcttc agcagcccgg gcaacctggc
     1141 caggtccgtg tccgcatgca ggacgcagac gatcgtgccc gtctccgcga tggtctccca
     1201 gaggggcatc gtctcgtccg tcccgaacca cgaccccggc gccggattct ctccctcgcg
     1261 gagcggtcgc aggcggattc cggtgacccc ccggttgcgc catttcttca gctcggcggg
     1321 cgccgccgga ccccgcggat cgatcacgca caccccacgg aatctgtccg gatcccgtcc
     1381 aatgcaggcc gccaggtagg agttatccca gtggtagaac cagggctgga cgaacacggc
     1441 cttctcgacg cctgcctcat ccatgtagtc catgaacaac tcgaccgagg ctgaggcctg
     1501 ggggctgtac ccgacacgca tcgggcccgg atccttaccg gcagcggatt catcccacac
     1561 atgcaggtgc gagtcgatga ctgggttcaa ggtcctgcct cccgtctcgt gcacgcgagg
     1621 cgggccagat aatggccctc ctcttcccgc cagatgatct cggcggacca gccttccctg
     1681 acggctcgag cggccagggt ctcgaagtcc acgaagagcc actccagggt cgggcccatc
     1741 ctcccctcgt attcgagttg aaagcgcatc tctccaggat acccgcccgt gcccgaggga
     1801 ccgccctgcg cggcgacgcc ttgggcagcc cctggccccg gatcgtagga atccaggagg
     1861 atctggcccc caggcctcag cagccgcttc acgtccctca gaaaccgctg aaggccggcc
     1921 aggctgccga cgaccccgat gccgttcatc aacatgagca gcgtatcgaa gggctccgcc
     1981 cggaagccga agacatcggc gcagcggacg tccctgacgc cccgcttccg catgacttcc
     2041 acggcttcgg gggccacgtc gatggcgcac acgtgtagcc ggcgctcttg caggaccagg
     2101 ctgtggcagc ctgcgcccgc ccccacatac aggacccgcc cccggcaaag gtccagcgcg
     2161 gcctcctcca gagccgaaaa ttcctcggcc tctcggaaga acacgcgtgc gggcacgacc
     2221 tccgtctccc cgtcggcctc gtggaccacg acctcggcgg aggtcctccc gttgaaatag
     2281 tccagcaggg cgcgcccgtg ggggctccag ggtttccgat ccatgccacc gcccgactga
     2341 gctcgccccg aagccgcctc gaggccttga tgctgtcgga ggagcggagg gaggccgtga
     2401 gacgggtgct acgggagcag aatcgccttg atgtaatcct ggggcggagc ggtcacaagc
     2461 tcttggaaga cccgcgcccc ctcagtgaga gggaacgtgc ggacccaggg cgtcaactcg
     2521 atcttccccg tagcgaacag accgatggcg gtccgaaggt ctcgaggggt cacggcaaag
     2581 gagccgctta tccgtcgctc ccccaacacc accgcgaagc ctgatacctg agtcgggtcg
     2641 tcgtgcaagc cgagccaaat gatgtccccc cccggacggg tggccatcac ggcggcgtgg
     2701 cgggtctctg cgacccccac ggcatccacg gccacgtcga tcccccggcc atgggtaaag
     2761 tcccggctgg cggcgaggat gtcttccttt cgcgggttga ctgtgatgtc ggctccgagc
     2821 cggcgcgcta cctccagtct ggcgtccgac acgtccgcca tcgccacccg gaatacgcca
     2881 gccgtcctgg ccacctgcaa tacgaagagc ccgatggtcc cggccccgaa gatgagcagc
     2941 gtctccggaa accgcaggcg aatctgagac agcgcgtgaa cggcgttggc cagcggctcc
     3001 gtgagggcgg cttggaggaa ggagacattc gctggcaatg gcaagagggt gctggctggc
     3061 gccgcgcaca gttcggcgaa ggcacccgat cgcttcgtcc cgtagacctc gccctcgcgg
     3121 cagaggtgac tccggccatc gaggcactcc tcgcagcgcc cgcaggcgat gaccgagttg
     3181 accgccaccc gatcgccggg ccgatagtct gtgacgccag ctccgttcct cgccacggtg
     3241 ccgcagaact cgtggcccat gatgagcggc ggcgtccggc gggccgcgtg cgtgacatac
     3301 ccgtggagtt cggagccgca gatccccacc gcctccacct tgacgatcac ctcgccgggt
     3361 ttgggggtcg gctcggccac ctcgcgaatt tccagtcgat cccaggcagg atagaagagt
     3421 gctttcattt catcaggctc atcccacagg tgtc
//
LOCUS       DBNGZC010000184         2315 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1678082, whole genome shotgun sequence.
ACCESSION   DBNGZC010000184 DBNGZC010000000
VERSION     DBNGZC010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1678082"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="AC16G0_03410"
     CDS             <1..391
                     /locus_tag="AC16G0_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098702.1"
                     /translation="DRRMLSEVRRVLSPDGTFLCSTPNRLLVHPGSSLHARPLNPFHV
                     REYDPTEFRSLLRETFSTVRLYGQTRVPGWYASLLRGIGRTSPTLAARCHQVWKLVAL
                     SGMDEAIHWPMPLEVNEHAEILIGVCR"
     gene            388..1365
                     /locus_tag="AC16G0_03415"
     CDS             388..1365
                     /locus_tag="AC16G0_03415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5098703.1"
                     /translation="MTWCSVVMPIYNGARYLPHALDSLVAQHVADLEVIAVDDASTDE
                     SLDILNAYRDRLNLTILSRPHTGNWVQNTNDGIRLARGEYIGILHQDDLWAPDRLDQL
                     RRLARALPDAGMYFHAATYIDADGRPLGLLTAPLKPALVYPRTTILPQLLVQNWIAMP
                     TVLVKAEYARFIHEDLWYSADWLQWLRIAEQAPIVYWPTPLASFRLHPASQTARRRKD
                     IRDQLERVLRCRPDDPVARFSMEVNVALAGRPSWRLLPQAIRLSLGDWSRYDRDSRIR
                     ERASARLRAWWRDVSRRELSTGTAHAATHPAPDGASRFRFSGGSSPGPR"
     gene            complement(1460..1942)
                     /locus_tag="AC16G0_03420"
                     /pseudo
     CDS             complement(1460..1942)
                     /locus_tag="AC16G0_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(1945..>2315)
                     /locus_tag="AC16G0_03425"
     CDS             complement(1945..>2315)
                     /locus_tag="AC16G0_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098704.1"
                     /translation="PAGAPAVTWTWTYYPGLVAGQLPGSIQPDTTEARKIERLTIFGS
                     AIGPPYGLGGDPSVALPALVGEVITLSDPLDVSRQTWALYRISKIKARGQGVDLIVDF
                     IDVQAEPFSGSAMRLISVEF"
BASE COUNT          418 a          794 c          697 g          406 t
ORIGIN      
        1 cgaccgccgg atgctctccg aagttcgacg ggtgctgtcc cctgacggca ccttcctctg
       61 ctcgaccccg aaccgactgc tggtccatcc cgggtccagc cttcacgccc ggccgctcaa
      121 ccccttccac gtccgagagt atgatccgac cgagttcagg agcctcttgc gggagacttt
      181 ctccacggtc aggctctacg ggcaaacgcg ggtgccgggc tggtatgcga gccttcttcg
      241 aggcatcggg cgcacgtccc cgaccctcgc ggcccggtgc catcaggtct ggaaactcgt
      301 cgcgctctcg ggcatggacg aggcgatcca ctggccgatg ccgctggagg tcaacgaaca
      361 cgccgaaatc ctgatcggag tctgccgatg acgtggtgct cggtcgtcat gccgatctac
      421 aatggagcgc ggtatcttcc tcacgccctg gacagcctcg tcgcccagca tgtcgccgac
      481 cttgaggtga ttgccgttga tgacgcctcg acggatgaga gcctggacat cctgaatgcg
      541 taccgagacc ggctcaatct cacgatcctc agccggcccc ataccggcaa ctgggtccag
      601 aacaccaatg acggcatccg cttggcccga ggtgagtaca tcggcatcct gcatcaagac
      661 gacctctggg cgcctgaccg cctcgatcag ctccggcgtc tcgcccgagc acttcccgac
      721 gccgggatgt acttccacgc ggcgacctac attgatgcgg acggccgtcc tctgggtctc
      781 ctgaccgcgc ccctgaagcc ggcgctcgtg taccctcgaa cgactatcct ccctcagctt
      841 ctcgtccaga actggatcgc gatgcccacc gtgctcgtca aggcagagta tgcccgattc
      901 attcacgagg atctctggta cagcgccgat tggctgcaat ggctccggat tgccgagcag
      961 gcgcccatcg tctactggcc cacgcccttg gcctccttcc gtctccatcc agcgtctcag
     1021 actgcacggc gacggaagga catccgagac cagctcgaac gagtcttgcg ctgtcgccca
     1081 gacgatcccg tcgcccgctt ttcgatggag gtgaacgtgg cgctggccgg gcggccctcg
     1141 tggcgattgc tgccccaggc catccggttg agcctcgggg actggtctcg gtatgaccga
     1201 gactcccgga tcagggagcg ggcgagcgca cggctgcggg cgtggtggcg cgacgtttct
     1261 cgacgcgagc tttcaacagg aaccgcacat gccgccacgc atcccgcacc ggatggagct
     1321 tcgaggttcc gattctcggg tggtagttca cccggacctc gctgatccgt agacccgctc
     1381 tcaccgcccg cacgaccatc tccgaggcga actccatgcc cccagtctgg cagtgcatga
     1441 tctcccaggc gtcccgggtg aaggcccgca tcccgcaatg ggcatcgccg atcggcgcct
     1501 gaaagagcac gcgcaagagg ccgctcagga tcggattgcc aaggtagcga tgggtccagg
     1561 gcatcgcgcc agccatcatc ctccccttga agcggttgcc gatgatgacc tgctcgccga
     1621 ggtcgagaag cttcgtcatg tggtacagcg tgaagccatc gcaaaaggga taactcccgt
     1681 cggcatccgc cataatgatc caggggtatc gcgcaacccg caccccagcc tggatcgcat
     1741 agccatagcc ccgcggcctg gcctgaacga ccgtggcgcc catcgcctcg gccatcgagg
     1801 ccgaccgatc cgtcgagccg ttgtcggcga cgatgagttc ataggttagg ccaagatcat
     1861 cacacgcctt gatggcgtcg ggcagacacg cctgaagccc ctcgacctcg ttcaagcacg
     1921 gaaagacgat ggagaccggc atcgctagaa ctccacgctg atgaggcgca tcgctgaccc
     1981 cgagaagggc tcggcctgga cgtcaatgaa gtccacgatc aggtccacac cctgcccgcg
     2041 ggccttgatc ttgctgatgc ggtagagcgc ccaggtctgg cgggagacat cgagcgggtc
     2101 cgagagcgtg atgacctcgc cgaccaaggc tggcaaggcc accgaggggt caccgccaag
     2161 gccatacggc ggaccgatgg ccgagccgaa gatcgttaat cgttcgatct tccgcgcctc
     2221 cgtggtatcc ggctgaatcg agccgggcag ttgcccagcg acaagtccag ggtagtaggt
     2281 ccaggtccag gtcacagcag gagcccccgc tggcg
//
LOCUS       DBNGZC010000185         2121 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1287464, whole genome shotgun sequence.
ACCESSION   DBNGZC010000185 DBNGZC010000000
VERSION     DBNGZC010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1287464"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="AC16G0_03430"
     CDS             <1..899
                     /locus_tag="AC16G0_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098705.1"
                     /translation="GRGFWHWSRPEQELFKRDAILERLKQDSQPFRILDAGGFYPSGG
                     FSHALMRHEIAQVLGTSAVEIRFYDDLLGGRNVWNNLRYQNLWRLLAVRYVLIGDTAH
                     IPGYHKVLGPTMTSGGRPGYLYEADTIPPYARVIPAAVKGDTDRIVPTLLDPRLDYNR
                     LLLYLPNEPINPLPVTEMPTPSPSQARITRWAPGHITVALDPAPPAASYLLVAENWYP
                     DWHATVDGAAAQVLRGDYTFLSVPVGAGARTVDLWFDSADYRHGRMLTWISLLALAGW
                     GAGGGAIALRRRSGGGSGTAGG"
     gene            892..1617
                     /locus_tag="AC16G0_03435"
     CDS             892..1617
                     /locus_tag="AC16G0_03435"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HIR5098706.1"
                     /translation="MADQQALVVIPTYNEAPNIASLIPQVLAQDPRLEVLVVDDNSPD
                     GTGGIVEDLAKQNPRIHILHREGKQGLGTAYRAGFAWALERHYEFIFEMDADFSHDPA
                     HLKSFLTAIEDADLVLGSRYLQGKVTVVNWPMGRLLLSYFANVYARWVTGLHIWDLTG
                     GFKCFRRRVLEAIDLKQVRSNGYAFQIEMSVRAWRNGFKLAEVPIVFVDRTEGQSKMN
                     RGIVKEAVWMVPRLRLMAWFGRI"
     gene            1638..>2121
                     /locus_tag="AC16G0_03440"
     CDS             1638..>2121
                     /locus_tag="AC16G0_03440"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF013813.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR5098707.1"
                     /translation="MTGSGNDFVMVDGSVSTPDDWTEADIQAVCARGTGIGADGVVFV
                     GPGNSPGAVRMIYFNSDGSRASMCGNAALCSTRLASYLGVGPSRGMQLETDAGTYLSR
                     CVEEPDRAEIHLNPVEPPTEPALELEPGEERAGLTTVGVPHLVVLVRDVDQVDLVRRG
                     R"
BASE COUNT          360 a          676 c          720 g          365 t
ORIGIN      
        1 ccgggagggg attctggcat tggagtcggc cggagcagga gctgttcaag cgcgatgcga
       61 tcctcgaaag actgaaacag gattcccagc cgttccgcat cctcgatgcc ggagggttct
      121 atccctcggg cgggttcagc cacgcgctga tgcgacacga aattgcgcag gtgctcggga
      181 cgagcgccgt ggaaatccgg ttctatgacg acctgctggg cgggcgcaac gtctggaaca
      241 acctacgtta ccagaacctg tggcggctcc tcgccgtgcg ctacgtcttg atcggcgaca
      301 cggcgcacat tcccggctat cacaaagtgc tcgggcccac gatgacctca gggggacgcc
      361 ccggctatct gtacgaagcc gatacgatcc cgccctacgc ccgggtgatc cccgccgcgg
      421 tgaaggggga caccgatcga atcgtgccga cgctgctcga tccgcggctc gattacaatc
      481 gtctactctt gtatctcccc aacgagccga tcaatccgct cccggtcacg gagatgccga
      541 ccccgagccc gtcgcaggcc cgaatcaccc ggtgggcgcc cggccatatc acggtcgcgc
      601 tcgatccggc cccgccggcg gcgagttacc tgctcgttgc ggagaactgg tatccggact
      661 ggcacgccac ggtcgacgga gccgctgccc aggtgctgcg cggggattac acgttccttt
      721 cggttccagt gggggccggg gcccgcaccg tcgacctctg gttcgattcg gcggattacc
      781 gccacggccg aatgctgacc tggatttcgc tcctcgcgct ggccggatgg ggtgccgggg
      841 ggggggccat cgcgttgcgc cggcgcagcg gtggagggag tgggaccgcc ggtggctgac
      901 cagcaggcgc tcgtcgtcat ccccacctac aacgaggccc ccaacatcgc gagcctcatc
      961 ccccaggtgc tggcccagga cccgcgcctg gaggtgctcg tcgtggacga caactccccc
     1021 gacgggaccg gcgggatcgt cgaggacctg gcgaaacaga acccccgcat ccacatcctg
     1081 caccgggaag ggaagcaagg actcggcacc gcctaccgag ccggcttcgc ctgggccctg
     1141 gagcgccact acgagttcat cttcgagatg gatgccgact tctcccacga cccggcccac
     1201 ttgaagtcgt ttctcacggc catagaggat gcggatctgg tgctcgggtc gcgttacctt
     1261 cagggcaagg tcacagtcgt taattggccg atggggcggc tgctgctgtc gtacttcgcc
     1321 aacgtttacg cccgctgggt gacggggctg cacatttggg acctcaccgg tgggttcaag
     1381 tgttttcgca gacgagtgct ggaagcgatt gatttgaagc aggttaggtc gaacggctac
     1441 gctttccaaa tagagatgag cgtgcgcgca tggcgaaacg ggttcaaact ggccgaagtc
     1501 ccgatcgtgt tcgtcgaccg gaccgaggga cagagcaaga tgaatcgcgg catcgtgaag
     1561 gaagcggtct ggatggtgcc gcgcctgaga ctcatggcgt ggttcggccg catctgatcg
     1621 gtctccccgt ctacaaaatg acggggtcgg gcaacgattt cgtgatggta gacgggagtg
     1681 tctcgacccc ggacgactgg accgaggcgg atatccaggc cgtgtgcgcg cgggggacgg
     1741 ggatcggggc cgacggggtc gtctttgtgg gcccggggaa cagccccggc gccgtccgca
     1801 tgatctactt caacagtgac ggctcccggg cttccatgtg cggcaacgcc gccctgtgca
     1861 gcacccggct ggcgagttac ctcggcgtcg gtccctcccg agggatgcag ctcgagaccg
     1921 atgccgggac ctacctgagc cgctgcgtcg aggagccaga ccgggcggaa atccatctga
     1981 accccgtaga gcctcccacg gagcccgcgc tggagctgga gcccggtgag gagcgcgccg
     2041 ggctgaccac agtgggggtg ccgcacctgg tcgtgctcgt gcgcgacgtg gaccaggtgg
     2101 atctggtccg gcgcgggcgg g
//
LOCUS       DBNGZC010000186         4438 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2166931, whole genome shotgun sequence.
ACCESSION   DBNGZC010000186 DBNGZC010000000
VERSION     DBNGZC010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2166931"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..888
                     /locus_tag="AC16G0_03445"
     CDS             148..888
                     /locus_tag="AC16G0_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012473590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaeR7I family type II restriction endonuclease"
                     /protein_id="HIR5098708.1"
                     /translation="MATSGDDLATAVRSAVRHFWRTRARQAKAQGAKSGVRDRGARSA
                     VTGGAHLDGFAELIRKLIVDAGVSDAAVHRRTQVELPGYYRAEKKWDLVVVVDGRLFA
                     TVEFKAQVGPSFGNNYNNRTEEALGNATDYWAAFREGAFRGSPKPWLGYLMLLEDATG
                     SMRPVSVVEPHFPVFDEFRDASYALRYEILLTKMVRERLYDAASLILSPSDEGRNGAF
                     SEPCSELRFANFAESLVASAIAYSRTQK"
     gene            1050..1157
                     /locus_tag="AC16G0_03450"
     CDS             1050..1157
                     /locus_tag="AC16G0_03450"
                     /inference="COORDINATES: protein motif:HMM:NF025088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098709.1"
                     /translation="MFAEPGAPHHRPHFHAYYQNQVGIFANRRHRMYRR"
     gene            1159..1281
                     /locus_tag="AC16G0_03455"
     CDS             1159..1281
                     /locus_tag="AC16G0_03455"
                     /inference="COORDINATES: protein motif:HMM:NF025088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098710.1"
                     /translation="MQQTQERLVEAWTELHRAEYLENWNRLHAGRRPLRIAPLH"
     gene            1296..1574
                     /locus_tag="AC16G0_03460"
     CDS             1296..1574
                     /locus_tag="AC16G0_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HIR5098711.1"
                     /translation="MRHPVYRVTGFEVVGPHTLRVEFDDATTQVIDFGDVLAGEIYGP
                     LKDEQLFRSVEIDPEVHTLVWPSGADFDPAVLHDWPAHANGMRRLAKC"
     gene            1707..2096
                     /locus_tag="AC16G0_03465"
     CDS             1707..2096
                     /locus_tag="AC16G0_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR5098712.1"
                     /translation="MQGDEEAIRKLIATWLSASKAGDTEKVLSLMADDVVFLVPGRPP
                     MRGKSAFASGQSALSEFSIEATSEIQEIKVFGDWAYCWTALTVVVTPRKGGAAVKRSG
                     NTLSILRKENGAWLLFRDANMLAVVSG"
     gene            2224..3444
                     /gene="coaBC"
                     /locus_tag="AC16G0_03470"
     CDS             2224..3444
                     /gene="coaBC"
                     /locus_tag="AC16G0_03470"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR5098713.1"
                     /translation="MLTGKRILLGVTGSIAAYKAVELLREFVKRGAVVQVVMTEAATR
                     FVAPLTFETLSRQPVLLDMFSLAYGSRIGHIEATQRADAFLVAPATAQTIARMALGLA
                     DDFLSCIFLASRCPVVVAPAMDSDMYQHAAVQQNLTTLRGRGVRVVEPGHGELASGLV
                     GYGRLAELQDIVAAVERALAPMRDLRGEIVLVTAGPTREALDPIRYLSNRSSGKMGYA
                     ISEAAANRGARVVLVSGPTAQAVPQGVDVVHVETAQEMYEAGLAKLTAATVVIKAAAV
                     SDYRPKRVADRKIKKDETVPEVTLEPTPDILAEVGKRKGTRIVVGFAAETEDLVVNAQ
                     KKLQRKRVDLMIANDVRQSGAGFDTDTNVVKILDAEGGVEELPLLSKHEVAERILDRV
                     VKLLARRQPPAERK"
     gene            3441..4145
                     /locus_tag="AC16G0_03475"
     CDS             3441..4145
                     /locus_tag="AC16G0_03475"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR5098714.1"
                     /translation="MTNDPREEVREIAGQLRAYLAAQLELGVEQVEARWPDTPATPTG
                     AALPRPTLPQVREELGECTRCKLHAGRTRIVFGVGNPKAKLVFVGEAPGADEDAQGEP
                     FVGRAGQLLTKIIQAMGMQREDVYICNIIKCRPPNNRTPESDEILACQPFLLKQLQAI
                     GPQFVCALGGPAAQTLLQTKEPISRIRGKFYDFHGIPLLPTFHPAYLLRNPHEKKTVW
                     EDMKLLLQKMREVPTP"
     gene            4170..>4438
                     /locus_tag="AC16G0_03480"
     CDS             4170..>4438
                     /locus_tag="AC16G0_03480"
                     /inference="COORDINATES: protein motif:HMM:NF036997.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N' family DNA-binding
                     protein"
                     /protein_id="HIR5098715.1"
                     /translation="MTSNGRHFVDVVFPTTLEGPLTYRVPEEWKGSVFPGKRVLVPLG
                     PRTVTGYVVGVRDKPPVADAKDLREVLDPEPLLDAHLLELTRWVA"
BASE COUNT          865 a         1315 c         1509 g          749 t
ORIGIN      
        1 tggcagcagc gcgctgcggc cgccatagca tcggctccga ggttgatggg cactacttcg
       61 agatggcaga gcggcgcatc cgagatgaaa ccactgggct gttttccacc gttgcgatcc
      121 aagtccacaa agagtaggca caaccagatg gcaacgagtg gcgacgatct tgcgaccgcg
      181 gtccggagtg ccgtgcgtca cttctggagg acccgggcgc ggcaggcgaa ggctcaggga
      241 gcaaagtcgg gagttcggga tcgcggagcc cgaagtgcgg tcacgggcgg tgcccacctg
      301 gatggcttcg ccgaactgat ccgaaagctc atcgtggatg cgggtgtcag cgatgccgcc
      361 gtccatcggc gaacccaggt ggaattgccg ggatactacc gcgccgagaa gaagtgggac
      421 ctagtggtcg tagtcgacgg ccgtctattc gcaaccgtcg agttcaaggc gcaagttggt
      481 ccatcgttcg ggaacaatta caacaacaga acggaagagg cgctcgggaa cgcaacggac
      541 tactgggcag ccttccgtga aggcgctttc cgaggatcac ccaaaccgtg gctggggtac
      601 ttgatgctcc tggaggacgc gacgggctct atgcgcccgg tgtctgtagt ggagccgcac
      661 ttcccggtgt tcgacgagtt tcgcgacgct tcttatgcac tgcgatatga gatcctactc
      721 accaagatgg tccgggaacg gctgtacgac gcggcgagtc tcattttgtc gcccagcgat
      781 gaagggcgta acggggcgtt ctcggaaccg tgctcggaat tgagattcgc gaacttcgcc
      841 gagagcctcg ttgcgagtgc gatcgcatac tccaggacac agaaataatc cgcagtcgca
      901 tctgctgcct gccggccaac ctctcgttgc accgggcggc ctatggcagc cggtgatccc
      961 ggacgtttgg cgcaggactt ggcggaagcg gggtaccagg gggataatgg gtgccagaga
     1021 ttgcccggtt ctacggaatc atcatcagga tgtttgccga accaggtgca ccacaccacc
     1081 ggccacactt tcacgcttac tatcagaatc aggtggggat attcgccaat cgacgacatc
     1141 gaatgtatcg gcggtgaact gcaacaaacc caagagcggc ttgtggaggc gtggactgag
     1201 cttcaccggg cagagtacct ggagaactgg aaccgactgc atgctggcag gcggccgctc
     1261 aggatcgcac cgcttcacta agactgggag tgctgatgag acatccggtc taccgagtca
     1321 ccggttttga agtcgtcgga ccacacacgt tgcgggtgga gttcgacgac gcgaccactc
     1381 aagtgatcga cttcggcgat gtgctcgcgg gcgaaatcta tggtccgctg aaagatgagc
     1441 aattgtttcg cagtgtcgaa atcgatccgg aagtccatac actcgtctgg ccgtccggtg
     1501 cagacttcga cccggccgtg ctccacgatt ggccagcgca cgcaaacggg atgcgacgat
     1561 tggccaaatg ctagggagcg gcgggatcca cccatcgtcg cgcgagccgg ccgcctagca
     1621 ggcgcatgca tccgccgcga aagaaagccg tgtgcggctg atgcgcatca cgttagccac
     1681 cacgagagcc agctcttgga gttcccatgc aaggtgacga ggaggccatc cggaagctca
     1741 ttgcaacatg gctcagtgcc tcgaaggccg gagacaccga gaaagtcttg agcctgatgg
     1801 cggacgacgt tgtgttcttg gtgcccggtc ggccacccat gcgcggcaag tctgcgtttg
     1861 catcgggtca gtcagcgctc agcgagttca gcatcgaggc aacgagcgag attcaagaga
     1921 tcaaggtgtt cggtgattgg gcgtattgct ggaccgccct gactgttgtc gtcacacctc
     1981 ggaagggggg cgcggcagtc aagcgctcgg gcaacacgct ctccatcttg cggaaggaaa
     2041 atggcgcctg gctgctcttc cgcgacgcca acatgctggc ggtcgtttca gggtagcgcc
     2101 aaggacgtct tggaagagat tcgtgatcgg cacgggcggc ttccatgaca aggtatctga
     2161 gtctggatgg ttgcatcgga ggttcatgaa cggaatttcg cgtcatgaga acggggtgag
     2221 gccatgctaa ccggcaagcg aatcctactc ggagtcaccg gaagcatcgc ggcgtacaag
     2281 gcggtggagt tgctgcgcga gttcgtgaag cggggggccg tggtccaggt ggtgatgacc
     2341 gaggcggcga cgaggttcgt ggcgcccctc accttcgaga cgctgtcgag gcagccggtc
     2401 ctcctggaca tgttctccct ggcctacggc tcgaggatcg gccacatcga ggccacgcag
     2461 cgggcggatg ccttcctggt ggcgcccgct acggcgcaga caatcgcccg catggccctg
     2521 gggctggccg acgatttcct gagttgcatc ttcctggcca gccggtgccc cgtggtcgtg
     2581 gcgcccgcca tggacagcga catgtatcag cacgcggccg tccagcagaa tctgaccacg
     2641 ctgcggggac ggggtgtccg cgtcgtggag ccgggccatg gcgagctggc gtcgggcctg
     2701 gtcgggtacg ggagactcgc ggagctgcag gacattgtgg cggccgtcga gcgggctctg
     2761 gcgccgatgc gtgacctgag aggagagatc gtcctggtga cggccggacc cacgcgggag
     2821 gcgcttgacc cgatcagata cctgtcgaat cgctcctcag ggaagatggg ctacgccatc
     2881 tccgaggccg cggcgaatcg tggggcccgc gtggtcctgg tgagcggacc gacggcacaa
     2941 gctgtcccgc agggcgtgga cgtggttcac gtggagacag cccaggaaat gtacgaggcg
     3001 ggtctggcca agctcacggc agcgaccgtg gttatcaagg cggcggcggt gtcggactat
     3061 cggccaaagc gggtggcgga tcggaagatc aagaaagatg agacggtccc cgaggtcacc
     3121 ctcgaaccca cgccggatat tctggccgag gtcgggaagc ggaagggcac gcggatcgtg
     3181 gtgggcttcg ccgccgagac ggaggacctg gtggtcaacg cgcagaagaa gctccagcgg
     3241 aagcgcgtgg acctgatgat cgcgaacgac gtccgccagt ccggcgccgg cttcgacacg
     3301 gacaccaacg tggtcaagat cctggacgcc gagggggggg ttgaagagct gccgctgctc
     3361 tcgaagcacg aggtggccga gcggatcctg gatcgggttg tgaagcttct ggcacgacgc
     3421 caaccgcctg cggagcgcaa atgacgaacg atcctcgaga agaagtgcgc gagatcgctg
     3481 gacagctccg ggcctacctt gcggcccaac tcgagttggg cgtggaacaa gtcgaggccc
     3541 gctggcctga tacgcctgcg acccccacgg gggcggcgtt gccacggcct accctgcccc
     3601 aggtgcgcga ggagctggga gaatgcacgc ggtgcaagct gcacgcgggc cgcacgcgaa
     3661 tcgtcttcgg ggtcggcaat cccaaggcca aactggtctt cgtgggcgag gcgccggggg
     3721 ccgatgaaga cgcccaggga gagcccttcg tggggcgcgc gggacaactc ctcaccaaga
     3781 tcatccaggc catggggatg cagcgggaag acgtgtacat ctgcaacatc atcaagtgcc
     3841 gtccgcccaa caaccggacc cccgagagcg acgagatcct ggcgtgccag ccgttcctgc
     3901 tgaagcagct ccaggccatc ggcccgcagt tcgtctgcgc cctgggagga ccggcggccc
     3961 agaccctgct ccagaccaag gagcccatct cgcggatccg cgggaagttc tacgacttcc
     4021 acgggatccc gctgctgccc acattccacc cggcgtacct gctgcggaac ccgcacgaga
     4081 agaagacggt ctgggaagac atgaagctcc tcctgcagaa gatgcgggag gtgcccaccc
     4141 catagacttg gacctcaggc ctcacagcta tgacgagcaa cggccgccac ttcgttgatg
     4201 tggtcttccc cacgacgctg gagggccccc tgacctaccg ggtgccggag gagtggaagg
     4261 ggagcgtgtt cccagggaag cgggtcttgg tccccctggg cccgcggacg gtgaccgggt
     4321 atgtggtggg ggtgcgcgac aaaccaccgg tcgccgacgc caaggacctc cgcgaggtcc
     4381 tggaccccga gcccctcctg gatgcccacc tcctggaact cacccgctgg gtggcgga
//
LOCUS       DBNGZC010000187         1644 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1776634, whole genome shotgun sequence.
ACCESSION   DBNGZC010000187 DBNGZC010000000
VERSION     DBNGZC010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1776634"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1433
                     /locus_tag="AC16G0_03485"
     CDS             231..1433
                     /locus_tag="AC16G0_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_416352579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR5098716.1"
                     /translation="MRKSINPLTPTQVYRYAVQACQPHLKLGGPKKALGSMILTVLFA
                     AAARVSSLSDTCRRLQGVPDEHVVADALYATPPGYDVLRRRVQAALRGHLPKPLRRKP
                     QVVALDLTLLPCYGADAKTDPLVVRAKAKKGTCSFFGYGTAYVVRTGWRYTLALTAVT
                     RDMTMADLACALLKQVRAAGIKVRFLVLDREFYSVAVIRYLQATRVAFLMPVVGHGRP
                     ADHPLGPSGSNVFKQCKRSGWSRYTLTDAAKRRATVRICIKCRNRRGGRGQQGREAWI
                     YAYWGIGPKRFDWVKGTYRRRFGIEASYRQMNRCRIRTTSKRFAVRFLYVAIALVLRN
                     LWVWLHHAVLSRPRRGGIELHLERLRLRTMLLWLFEVAAEMYGLANMTLIEGLLPKIC
                     YNVGLRKP"
BASE COUNT          273 a          559 c          520 g          292 t
ORIGIN      
        1 ctccaggtgc aactggtcca ggtccaagca ggacaggttg cgcagttcga cctccttgag
       61 gtggcgccac atccactcca cggggttcag gtccggggcg tacgacggca accgctcccc
      121 gacgctcagt agttcggtag atcggcttgg agaggggtgc caggggatga cagactcaac
      181 ctgtcctccg agaatgactt gggcaaaccc taacgatcac ggagggtcct atgcgcaagt
      241 ccatcaaccc cctgacaccc acccaagtct atcgctacgc cgttcaggcc tgccagcctc
      301 acctgaaact gggcggcccc aagaaggccc tcggctcgat gatcctgacc gtcctgttcg
      361 ccgccgccgc gcgggtcagt tcgctgtccg acacctgccg gcgactccag ggcgtccccg
      421 acgaacacgt cgtcgccgac gcgctctacg ccacgcctcc cgggtacgac gtcctgcggc
      481 gacgtgttca ggccgcgctc cgcggccacc tacccaagcc cctgcgccgc aagccccagg
      541 tcgtcgccct cgatttgacg ttgctcccct gctacggcgc cgacgccaag accgaccccc
      601 tggtcgtccg tgccaaggcc aagaagggga cctgctcgtt cttcggctat ggcacggcct
      661 atgtggtccg cacggggtgg cgctacaccc tcgcgctgac cgccgtgacg cgagacatga
      721 ccatggccga cctcgcctgc gcactgctca agcaggtgcg cgccgccggg atcaaggtcc
      781 gcttcctggt cctggaccgc gagttttaca gcgtcgcggt catccgttat ctccaggcga
      841 cgcgggtcgc gttcctgatg ccggtggtcg gccacggccg cccggccgac catccgctgg
      901 gccccagcgg ctcgaacgtc ttcaagcagt gcaagcggag cggctggagc cggtacacgc
      961 tgaccgacgc ggcgaagcgc agggccacgg tgcggatctg catcaaatgc cggaaccgtc
     1021 gcggcgggcg tggccagcag ggtcgggagg cctggatcta tgcctattgg gggattggtc
     1081 cgaagcggtt cgactgggtc aaggggacgt accggaggag gttcgggatc gaggccagct
     1141 atcgtcagat gaaccggtgt cggattcgga cgacgtcgaa gcggtttgcg gtgcggttct
     1201 tgtacgtggc gatcgcgttg gtgctgcgga acctgtgggt gtggctgcat catgcggtgc
     1261 tctcccggcc gcgtcgcggc ggcatcgaac tccacctgga gcgcttgcgc ctgaggacga
     1321 tgctgctgtg gctcttcgag gtggccgccg agatgtatgg cctggcgaac atgacattga
     1381 tcgaaggact tttgccgaaa atctgttaca acgtaggtct ccgaaagcca tagtaccgaa
     1441 ctactgagat tctccagcgg cgccaaccgc cggcacaccc agctcttgtg ccggcccagc
     1501 aactccgccg cctcgctctg ggacaagccg tcctcgcgta ccaaggcttg aacaagccat
     1561 gcttcttccg actcgtgcgg ccgccgaccc gcctggttga gcccgtaaat cgccgcctcg
     1621 gcccgctggt cgtcaacgtc gatc
//
LOCUS       DBNGZC010000188         1888 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_16034, whole genome shotgun sequence.
ACCESSION   DBNGZC010000188 DBNGZC010000000
VERSION     DBNGZC010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_16034"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..992
                     /locus_tag="AC16G0_03490"
     CDS             <1..992
                     /locus_tag="AC16G0_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098717.1"
                     /translation="PRGRRGWWLHPFALPGRPGWLGLGSLVRPHSLPAVCGFPLPADR
                     RNAWTVPARDGWGRPLRDLDGQIIGLLRETDVYILFDLLGQEPKLACILTRTILDLSL
                     EAGRLLLPILTGLGPATLEARLRQLRQTTEVEAFRASAAWRADRQQGDVSESEIEALV
                     NDLQELERDLRTSGRQMQSLERRLTLADRRLTDLGQLQASEEALLRDFDRLVGLPGVT
                     DVEIRDGVLRVFTDVIVVTYGGRQYRMGRFRLDLHFDGRVFLRNLTDRYETYDHPHVE
                     NGRPCLGNIQEWMQRLLAQREFVAACEILLHYLRTVNPGDWRKAVTFWPEVGE"
     gene            1004..1540
                     /locus_tag="AC16G0_03495"
     CDS             1004..1540
                     /locus_tag="AC16G0_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098718.1"
                     /translation="MAPQIRIRQEALAKLLLYAERCPMEIGGLGYVLQDEEGLFIPDL
                     FVLPQKVSASDTELDPDALCEFLGRLVREGVDVSSVRLWWHSHGDMDLIWSETDCLTI
                     GDLPGDFWVAIVVNRRGEIRCRLDAYAPQRRTWDVSLLEIPEEAGCDREVLRAAIDRE
                     ICENVRACTLVQDAMVME"
     gene            1641..>1888
                     /locus_tag="AC16G0_03500"
     CDS             1641..>1888
                     /locus_tag="AC16G0_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098719.1"
                     /translation="MRQWAMMMTLLVMALPGFRAEAAAPGTGEPDPGRVVARVNQTEI
                     TYGTFEARLEDLQQERGPIPPDKRGELLRALVREEVLA"
BASE COUNT          314 a          572 c          680 g          322 t
ORIGIN      
        1 ggccgcgcgg gcgacgcggc tggtggcttc atccctttgc gcttccggga cggcccggat
       61 ggctggggct gggctcgctg gtgcgtcccc atagcctccc ggccgtctgc ggcttcccgc
      121 taccggcgga ccgccgcaac gcctggaccg ttccggcgcg ggacggttgg ggacgtcctc
      181 tgcgggatct cgacggacaa attatcggcc tgctgcggga gacggatgtg tacatcctct
      241 tcgatctcct ggggcaggag ccgaagctgg cctgcatcct cacgcgcacg atcctggacc
      301 tcagcctgga agcggggcga ctcctgctgc ccatcctgac cggcctcggc cccgcgacgc
      361 tggaggcgcg gctgcggcaa ctccggcaaa cgacggaggt cgaggcgttc cgcgcctctg
      421 ccgcctggcg ggcggaccgc cagcaaggcg atgtctcgga gagcgagatc gaggccctcg
      481 tcaatgactt gcaggagcta gagcgggatt tgcggacctc gggacgccag atgcagagcc
      541 tggagcggag actcacgctg gcggatcggc ggctcacgga tctcggtcaa ctccaggcct
      601 cggaagaagc cctgctgcgg gacttcgacc ggctggtggg acttcccggc gtcaccgatg
      661 tggaaatcag ggacggcgtg ttgcgggtct tcacggacgt gatcgtcgtc acctatgggg
      721 gtcggcagta ccggatggga cggttccgcc tggacctaca cttcgacggc cgggtgttcc
      781 tgaggaactt gacagaccgg tatgaaactt acgatcaccc gcacgtggag aacgggcgac
      841 cgtgcctcgg gaacattcag gaatggatgc agcgcctgct ggcgcagcgg gagttcgtcg
      901 cggcctgcga gatcctgctg cactacctcc gcacggtgaa tccgggcgac tggcgcaagg
      961 cggtaacatt ctggccagag gtcggcgagt gacccttccg cccgtggccc cgcagatccg
     1021 catccgccag gaggccctgg ccaagctcct gctgtatgcg gagcgatgcc cgatggaaat
     1081 cggcgggctc gggtacgtcc tccaggacga ggaaggactg ttcatcccgg atctgttcgt
     1141 cttgccgcag aaggtgtcgg cctcggacac ggaattggac cccgacgccc tgtgcgagtt
     1201 cctgggacgg ctggtgcgag agggtgtgga tgtctccagc gtacgcctct ggtggcacag
     1261 ccatggggat atggacctga tctggagtga aaccgactgt ctcaccatcg gggatctgcc
     1321 gggggacttt tgggtggcaa tcgtggtgaa tcgtcgaggc gagatccgct gcagactgga
     1381 tgcctatgca ccccagcgaa ggacgtggga tgtctctttg ctcgagattc cggaggaagc
     1441 cggatgtgat cgcgaggttc tccgggctgc catcgaccgc gagatctgcg agaacgtgcg
     1501 ggcttgcacg ctggtgcagg acgcgatggt gatggagtga cacgcaggac cgggcgcgga
     1561 ttcccccagg gtcccggcag tgcaatctcc gcacgggcgg cgctcgccgg acggcgcggg
     1621 aaaagaaagg cgaggtaggt atgcgacagt gggcgatgat gatgaccctc ctggtgatgg
     1681 cgctgccagg attccgggcc gaagcggccg cgccgggcac cggcgagccg gacccgggcc
     1741 gcgtggtcgc ccgggtgaac cagacggaga tcacgtatgg cacgtttgag gcgcgtctgg
     1801 aagacctcca gcaggaacgc ggccctatcc cacccgataa acggggtgag ttgctgcgag
     1861 cgcttgtccg agaggaggtt ctggccca
//
LOCUS       DBNGZC010000189         1383 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_113549, whole genome shotgun sequence.
ACCESSION   DBNGZC010000189 DBNGZC010000000
VERSION     DBNGZC010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_113549"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1383)
                     /locus_tag="AC16G0_03505"
     CDS             complement(<1..>1383)
                     /locus_tag="AC16G0_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012636741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIR5098720.1"
                     /translation="IFPFTEDRKRETAIVRTEDGQLLAVTKGAAEVVLAMSTLDTPGR
                     AAWHDEIAWLAEGAHKVIAVASREADGLPASGGEPDSGFEFVGLLAFEDPVREGVRDA
                     VRDCLSAGIHPIMVTGDHPKTAVAVAKSVGIGGENPRLITGEELEGFVSHQKGDELLG
                     VDVIARARPAQKLMLVKSLQEGGEIVAVTGDGVNDVPALQAADVGVAMGERGTRSARE
                     VAAIVLLDDNFRTIVGAIREGRQLFENLRSSFQYLLMIHIPFVVTATLIPLAGYPLLY
                     LPVHIVWLEIMIHPTAMLAFQEPADRVLARRPHIAGRARFFGFDEWAVIVLTGIVLSV
                     MVMAGYLRSLEGVRNVEHGRAMALATFMVTGACVAAALNRLRTRASWLIAIGTILSSF
                     LLIQTPKMNRLLHLQALHGDDWALVGLAELFVGCLPFLRGLFATRVHPATDGAEPHRS
                     PRVSADVKVR"
BASE COUNT          257 a          459 c          434 g          233 t
ORIGIN      
        1 tcccgcactt ttacgtcggc gctaacacgg ggcgacctgt gaggttccgc accgtctgtg
       61 gcgggatgga cgcgagtggc gaagaggccg cgaaggaacg gcaggcaacc gacgaacaat
      121 tccgcgagcc cgacgagcgc ccaatcatcc ccgtggagtg cctgcaggtg gagcaggcga
      181 ttcatcttcg gcgtctggat caggaggaac gacgagagaa tcgtgccgat cgcgatcaac
      241 caggaggccc gagtgcggag tcggttgagc gcggctgcca cgcaggcccc cgttaccatg
      301 aaggtcgcca aggccatcgc gcggccgtgt tcgacattac gcacgccttc gagactccgc
      361 aggtagcccg ccattaccat cacggagagc acgatcccgg tgagcacgat gaccgcccac
      421 tcgtcgaagc caaagaaccg ggcccggccc gcaatgtggg gccttctggc gagcacccgg
      481 tctgcgggct cctggaaggc cagcatggcc gtcgggtgaa tcatgatctc taaccagacg
      541 atatggaccg gcaggtagag cagcgggtat ccggccaagg ggatcagcgt ggcggtcacg
      601 acgaaaggga tgtggatcat gagcagatac tggaaactgg accgcaggtt ttcaaacagc
      661 tgacgaccct cgcggatcgc ccccacgatg gtgcggaagt tgtcatccaa aagcacgatc
      721 gccgcgacct cccgtgcgct ccgcgttccc ctctcgccca tcgcgacccc gacgtcggct
      781 gcctggagcg cgggcacgtc attgacccca tccccagtga ccgcgacgat ttctccgcct
      841 tcctgcaaac tcttcaccag catgagcttc tgggctggtc tcgcgcgagc gatgacgtcc
      901 acgccgagga gttcgtcgcc tttttggtgg gagacgaacc cttccagttc ttctcccgtg
      961 atcagacggg ggttctctcc gccgatgccg acgctcttgg caaccgcgac ggccgtcttc
     1021 ggatgatcac cggtgaccat gatagggtgg atcccggcgg acaagcagtc ccggaccgca
     1081 tccctgacgc cttcgcggac cgggtcctcg aacgccagca ggcctacgaa ctcaaagccg
     1141 ctgtccggtt ccccgccgga tgccggcaag ccgtcggcct cacgggaggc aaccgcgatc
     1201 accttatgcg ccccttcggc gagccacgcg atctcgtcat gccaggcggc tcgcccggga
     1261 gtgtccaagg tggacatcgc caggacgacc tccgcggccc ctttggtgac ggcgagaagt
     1321 tgcccgtctt ccgtccttac gatggccgtc tcacgcttgc ggtcctcggt gaaggggaag
     1381 atc
//
LOCUS       DBNGZC010000190         5534 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2167081, whole genome shotgun sequence.
ACCESSION   DBNGZC010000190 DBNGZC010000000
VERSION     DBNGZC010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5534)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2167081"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="AC16G0_03510"
     CDS             complement(<1..670)
                     /locus_tag="AC16G0_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098721.1"
                     /translation="MPSERVTSLSPLGVPGWRARVWFREPVTLLVGLFLAGVLALFVL
                     WPILQVITFPPLTDYLHLHENSRWMRATSNTVRMVLLSTCTSTLIGFLYAMALARPDF
                     PGREFFRLVAILPLFSPPFTVGFSYLLMFGRFGVITHGLFGLEASILGWGSLWLVQTL
                     SNFPYAALVIERALSATPPTLEAAARDLGGGWPAVFRTITLPLARPAVAGAALLVAIY
                     ILADF"
     gene            complement(699..1727)
                     /locus_tag="AC16G0_03515"
     CDS             complement(699..1727)
                     /locus_tag="AC16G0_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5098722.1"
                     /translation="MRQRWVLGFAVVLGIVGGLAGLAAAQQKIVIYAAQDEKTTNEMT
                     RMFTEETKIQTEVIRVAAAGTLATRIRAEKAAPKADVFMGGSVEFHEPLATEGLVLPY
                     RSPVVEQAKIDSAYVSPQNFWHGFFMNSLVIILNPQRFQREMTSAGVKKPQTWDDLLN
                     PVYSKKVIAGNPATCGGAYIYTAVQIFRQGGETQGFDWLKKLDGQILQYTPTCPGTIT
                     LVTRGEAVAGITWADDSIEAMMQKQPIEVIFPPDTGAEIGGLSIIKGGPNPEGAKKWV
                     DFVLGKKAQALKTELGFTYPVRDDVDPPKNVPPLSAIKLGKYDRKWAIENRERVTKKW
                     ETEIGSKR"
     gene            complement(1803..2882)
                     /locus_tag="AC16G0_03520"
     CDS             complement(1803..2882)
                     /locus_tag="AC16G0_03520"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5098723.1"
                     /translation="MARIRIGVVGCGLIGQMMHLPHLKELHELYELVALCDVSPATLK
                     YVGDYYGVSKRHTDHHELLKEPVDAVVVLSADSHGAVIVDALKAGKHVFTEKPMCYTL
                     READEILAAQKKAGTICMVTYMKRFDPGFLYAQPLIRNLKNLQTSHITVLHPSEQNQI
                     GHHDIRRFMDVPADVRAALRAQQDSLIREVIGDFTPLERRVFADNLLSTLVHDINVLR
                     GLSGDPAEVLFTDVWHGGEAMLTSLRYASGVRAIISLQYLPDLANYEETVAYYAKADR
                     VRLIFPSPFFRNMPTPVIVEGMEEGKPFEKRVIASMKEAFKEEMLHFSECVQQGKPPI
                     TTAEEAKGDVALLHRIFKAIKRPLS"
     gene            complement(2969..3823)
                     /locus_tag="AC16G0_03525"
     CDS             complement(2969..3823)
                     /locus_tag="AC16G0_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR5098724.1"
                     /translation="MSLMPRFGVCAWIFGEAPLKETLGRIAAVGYDGVELPGEPEAYS
                     PAEVRRLLSWHGLAPLALTASCMAPETRRDLAHPDPAIRDEAVGYVVGCLRFAADIGA
                     PLVQMLPSGETRLSPLATRTEEWSWSIQGMRRAAREAERLRVRICIEPLNRYEAYLVT
                     TVEDALAYLTAVGSPWVGLTLDLFHAGIEEADLTDSIAAAGERLWHVHAADTNRQGLG
                     RGHLDLAGVTRALRGIGYSGSVVLEVMPPGPDPFRSIKDADSPKVLDAYLRESLGLLR
                     GHWTEKSA"
     gene            complement(3847..>5534)
                     /locus_tag="AC16G0_03530"
     CDS             complement(3847..>5534)
                     /locus_tag="AC16G0_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098725.1"
                     /translation="WQYLRSPIFLLLRHPELPLDPIRWGTAAGAAIGLTTLVLRWWAT
                     GRGVAGLSWGLGAGVGTALVSMLFFGLGALRNSLFLAAVVGCVSTGLGFVFALLESRS
                     RLWTRSLLGPLSILPIITPAFVLGLAMIYMFGRRGFITHSLLGLSTNAFFGPLGVGVA
                     QVLAFTPIAYLVLVGVIRSLDISLEEAARTLRADRWTLFRTILWPLVRPGLANAILLV
                     MIESLADFGNPLLLGGGTPFLPTEVFYAIEGRFDQHEAATYGLILLVITLTIFFLQRY
                     WLGRRSYVSVTGKPSGGHPTPLPLPVDLALTGVFLLFAGVAALLYGSIFFGSFVKLWG
                     IDNTFTLQNYRDLMSHGVPVLLDTMRLSLLAAFPSAGLGFLIAYLTLRHRFFGRTALE
                     FSAMLSYAIPGTVMGIGYILAFNAGALQLTGTAAIIVLAFIFRGMPVGIRSGVAALTQ
                     LDPSLEEVSATLKAGTTTTLRRVVMPLIRSAILTGLIYSFVRAMTAVSQVIFLVTPGH
                     DLVTVLILSWAEYGTIGRGAALSTLLILVLALCILPAHWLGRRRLIGVEAAAD"
BASE COUNT         1001 a         1784 c         1892 g          857 t
ORIGIN      
        1 cgaaatcggc gaggatgtag atcgccacga ggagtgcggc gcccgccacg gcgggccggg
       61 ccagcgggag cgtgatggtc cggaagaccg ccggccagcc tccgcccaga tcgcgggccg
      121 ccgcctccag ggtgggcggg gtggccgaca gagcccgctc gatgaccagc gcggcatagg
      181 gaaaattgct cagggtctgg accagccaca ggctccccca gccgaggatg cttgcctcca
      241 gtccgaagag cccgtgggtg atcaccccga agcgcccgaa catgagaagg taggaaaacc
      301 cgacggtgaa ggggggcgag aacagcggga ggatcgccac cagacggaag aactcccggc
      361 ccgggaagtc cgggcgggcg agggccatgg cgtacaggaa gccgatcagg gtcgaggtgc
      421 acgtggacag gaggaccatg cggacggtgt tgctcgtggc ccgcatccac cggctgttct
      481 cgtggaggtg gaggtaatcc gtgagcggcg gaaaggtgat gacctgaagg atgggccaga
      541 gcacgaagag ggccaggacg cctgcgagga acaggcccac cagcagggtg acgggctcgc
      601 ggaaccagac gcgggcccgc caccctggca cccccagagg ggacaggctg gtcacacgtt
      661 ccgatggcat gcgacctccc gtgtggccgg acggcacctc atcgcttcga accgatctcg
      721 gtttcccact tcttcgtcac ccgttcgcgg ttctcgatgg cccacttccg atcgtacttc
      781 cccagcttga tggccgacag cggtggaacg ttcttgggcg gatccacgtc gtcccggaca
      841 gggtaggtga accccagctc cgtcttgagg gcctgggcct tcttccccag gacgaaatcc
      901 acccacttct tggccccctc gggattcgga ccacccttga tgatggacag gccgccgatc
      961 tcggcgccgg tgtccggtgg gaagatgacc tcgatgggct gcttctgcat catggcctcg
     1021 atggagtcat cggcccaggt gattcctgcc accgcctcac cacgtgtgac cagggtgatc
     1081 gtgcccggac aggtcggcgt gtactgcagg atctggccgt ccagcttttt cagccagtcg
     1141 aatccctggg tctcgcctcc ctgccggaag atctggactg cggtgtagat gtacgcgccc
     1201 ccgcaggtgg ccgggttgcc cgcgatcacc ttcttgctgt acacgggatt cagaaggtca
     1261 tcccaggtct ggggcttctt gacgcccgcg gacgtcatct cccgctggaa ccgctggggg
     1321 ttcaggatga tcaccaggct attcatgaag aacccgtgcc agaaattctg cgggctgacg
     1381 taggccgaat caatcttggc ctgttccacc accggcgagc ggtaggggag gaccaggcct
     1441 tccgtggcca gcggctcgtg gaactccacc gagccgccca tgaacacgtc ggccttgggg
     1501 gccgccttct ccgcccggat gcgcgtcgcc agggtgccgg cggccgccac gcggatcacc
     1561 tccgtctgga tcttggtctc ctccgtgaac atgcgggtca tctcattggt ggtcttttca
     1621 tcttgggcgg catagatcac gatcttctgc tgggccgccg ccagacccgc cagccctccc
     1681 acgatcccta ggacaaccgc gaacccgagc acccagcgct gtctcatggt ctcctccccc
     1741 tttgccccgg tccgtgaacg atctgccggc cgggcgaacc ggaaccacga ccggcggtgc
     1801 gcctagctca gcggccgctt gatggccttg aagatgcggt ggagcagggc cacgtccccc
     1861 ttggcctcct ccgcggtggt gatgggcggc ttcccctgct gcacgcattc ggagaagtgg
     1921 agcatctcct ccttgaaggc ctccttcatg gaggcgatga cccgcttctc gaagggcttg
     1981 ccctcctcca tgccctcgac gatgacgggg gtgggcatgt tccggaagaa gggagagggg
     2041 aagatcagac ggacgcgatc ggccttggca tagtaggcaa ccgtctcttc atagttggcc
     2101 aggtcgggca gatactgcag gctgatgatg gcccgcacgc cggaggcata gcggaggctg
     2161 gtcagcatgg cctcgccgcc gtgccagaca tcggtgaaga gcacctccgc cggatcgccc
     2221 gacagtccgc gcaggacgtt gatgtcgtgc accagggtcg acaggagatt gtcggcgaac
     2281 acccggcgct ccagcggggt gaagtcgccg atcacttctc ggatcaggga atcctgctgc
     2341 gcccgaagtg cggcgcggac gtcggccgga acatccatga accggcggat gtcgtggtga
     2401 ccgatctggt tctgctccga cgggtgcagg accgtgatgt gactggtctg caggttcttc
     2461 aggttccgga tgagcggctg ggcgtatagg aagcccggat caaagcgctt catgtacgtg
     2521 accatgcaga tggtcccggc ctttttctgg gcggcgagga tttcatccgc ctcccgcagc
     2581 gtgtagcaca tcggcttttc ggtgaagacg tgcttgcccg ccttcagggc gtccacgatg
     2641 accgccccgt gggagtcggc gctcaagacg acgacggcat ccaccggctc cttcaagagt
     2701 tcgtggtggt cggtgtggcg cttgctcacg ccgtagtagt cgcccacgta cttcaacgtt
     2761 gcgggggaga cgtcgcacag cgccacgagt tcgtacagct catgcagctc cttcaggtgg
     2821 ggcaggtgca tcatctgacc gatgagtccg catccaacga cgccgatccg gatccgagcc
     2881 atgggtctct ccttgtgtgg tggtctgaca gtcccgcgga tatcgtggga tgagtcgcca
     2941 tggaccacga gcgctcaccc cgtgagcttc aagccgattt ctccgtccag tgcccccgca
     3001 ggaggccgag cgactcgcgg aggtaggcgt ccaggacctt cggggagtcc gcgtccttga
     3061 tggagcggaa cgggtccggt cccggcggca tcacctccag gacgaccgat ccggaatagc
     3121 cgatcccgcg aagcgcgcgg gtcacgcccg ccaggtccag gtgaccccga cccagtccct
     3181 gccggttcgt gtccgcggca tgcacgtgcc ataggcgctc cccggcggcc gcgatggagt
     3241 ccgtcaggtc ggcctcctcg atgccagcgt ggaagagatc cagggtcagg ccaacccagg
     3301 gcgatcccac ggccgtgaga taggccaggg catcctcgac cgtggtcacg aggtaggcct
     3361 cgtaccggtt cagcggctcg atgcatatgc gcacgcggag acgctccgcc tcgcgcgcgg
     3421 cccgccgcat gccctggatc gaccaggacc actcctcggt gcgggttgcg aggggggaaa
     3481 ggcgggtttc tcccgagggc agcatctgca ccaggggagc gccgatgtcc gccgcgaatc
     3541 gcaggcaccc gaccacgtac cccacggcct cgtcgcgtat ggccggatcc ggatgggcca
     3601 gatcccgccg cgtctccggg gccatgcacg aggcggtgag ggcgagcggc gccagcccgt
     3661 gccaggagag caggcgccgg acctctgcgg gagaataggc ctcgggctcg cccggcagct
     3721 cgaccccatc gtatcccaca gcggcgatcc gacccagggt ctccttgagg ggggcctccc
     3781 cgaagatcca ggcgcagaca ccgaagcggg gcatcaggct catgtcgttc acccgtctcg
     3841 cgcggttcag tcggctgcag cctccacccc gatgaggcgt cggcgaccca gccagtgcgc
     3901 gggcagaatg cacagcgcca ggaccagaat gagcagcgtt gacagggcgg ccccccgccc
     3961 gatggtgccg tactccgccc acgagaggat gaggaccgtg accaggtcgt gccccggtgt
     4021 caccaggaag atgacctggc tcacggccgt catggcgcgg acgaagctgt aaatcagccc
     4081 ggtgaggatg gcggaccgga tcagtggcat gacgacccgc cggagggtgg tcgtcgtgcc
     4141 cgccttgagc gtggccgaga cctcctccag gctgggatcc agctgcgtca gggcggccac
     4201 gccgctccgg atgcccacgg gcatgccccg gaagatgaag gccaggacga tgatggccgc
     4261 cgtcccggtg agctggagcg cccccgcgtt gaaggccagg atgtacccga tacccatgac
     4321 ggtgccgggg atcgcgtagg acagcatggc cgagaactcc agcgccgtcc ggccgaagaa
     4381 ccggtgccgc agggtgagat aggcgatgag gaatcccagc ccggccgagg ggaacgcggc
     4441 caggagcgac aggcgcatgg tgtccaggag caccggaacg ccgtgactca tgaggtcccg
     4501 gtagttctgc agggtgaagg tattgtctat gccccacagc ttgacgaagc ttccgaaaaa
     4561 gatggagccg tacaggaggg cagccacgcc ggcgaagagc aagaacacgc cggtgagggc
     4621 aaggtccacg ggcaatggta ggggcgtcgg atgccctccg gagggttttc ccgtcacgct
     4681 gacgtaggag cgacgcccca gccagtatcg ctggaggaag aagatggtca gcgtgatcac
     4741 gaggaggatg agcccgtagg tcgccgcctc gtgttgatcg aagcgcccct cgatggcgta
     4801 gaagacctcg gtgggcagaa acggcgtccc gcctcccagg agcagcggat tcccaaagtc
     4861 ggccaggctc tcgatcatga ccagcaggat ggcgttggcc agtcccggcc gcaccagggg
     4921 ccagaggatc gtccggaaga gggtccaccg atccgcccgc agggtccggg ccgcctcctc
     4981 cagggagatg tccagcgagc ggatcactcc caccaagacg aggtaggcga tcggcgtgaa
     5041 ggccaggacc tgcgccacac ccacgcccag ggggccgaag aaggcattgg tggagagccc
     5101 caggaggctg tgggtgatga agccgcgacg cccgaacata tagatcatgg ccaggcccag
     5161 gacaaatgcg ggcgtgatga ttggaaggat ggacaggggt cccaggagac tgcgcgtcca
     5221 cagccggctc cgggactcca gcagggcaaa gacgaatccc agacccgtcg agacgcagcc
     5281 cacgactgcg gccaggaaca ggctgttccg aagcgccccc aggccgaaga agagcatgct
     5341 gacgagagcc gtgccgaccc cggcccccag gccccaggac aggccggcaa cgccgcgacc
     5401 cgtggcccac cagcggagga cgagggtcgt gagaccgatc gccgcgccag cggcggtccc
     5461 ccagcgaatg gggtccagtg ggagttccgg gtggcgcagg agcaggaaga tcggcgatcg
     5521 cagatactgc caga
//
LOCUS       DBNGZC010000191         2394 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1678336, whole genome shotgun sequence.
ACCESSION   DBNGZC010000191 DBNGZC010000000
VERSION     DBNGZC010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1678336"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..963
                     /locus_tag="AC16G0_03535"
     CDS             76..963
                     /locus_tag="AC16G0_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5098726.1"
                     /translation="MFEIFGVPSSALFGQLLIGLINGSFYALLSLGLAVIFGMLNIIN
                     FAHGAQYMMGAFVAYFLSKYAGLGYWWALLLVPIVVGLFGIALERAMLRRLYHLDHLY
                     GLLLTFGLVLIIQGLFRNAFGSSGLPYRIPTTLEGGRNLGFMFLPNYRAWVVVFSLAV
                     CLATWFVIERTKLGSYLRAATENPDLVQAFGVNVPRMITLTYGIGVGLAALAGVLAAP
                     IYQVNPLMGADLIIVVFAVVVIGGMGSILGSIITGFALGVIEGLTKVFYPEASNTVIF
                     VLMAVVLLWRPAGLFGRTS"
     gene            1022..1963
                     /locus_tag="AC16G0_03540"
     CDS             1022..1963
                     /locus_tag="AC16G0_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008944214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5098727.1"
                     /translation="MTPEHIGVGVMVALFLVAPFVVYPVFLMKALCFALFACAFNLLI
                     GFVGLLSFGHAAFLGSAGYATAHAAKVWGFPPELAILFGTGVAALLGLIIGSVAIRRQ
                     GIYFAMVTLSLAQMIYFFSVQAPFTGGEDGIQGVPRGRMLGVLDLQNTLTLYFVVLGI
                     FLGGFLLIYRTIHSPMGQVLKAIRESEQRAISLGYNPDHYKLIAFVLSATLSGLAGGT
                     KSIVFQLASLTDVHWSISGEVVLMTLLGGLGTVFGPVAGALIVVTLENYLVQLGAWVT
                     VVQGAIFVVCVLVFRRGVVGELGRFVRKVQNPASANG"
     gene            2024..>2394
                     /locus_tag="AC16G0_03545"
     CDS             2024..>2394
                     /locus_tag="AC16G0_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5098728.1"
                     /translation="MLVKERMKRDPVTAKKDDSFRYALKLLRKEGIRHLPVLDGKRLV
                     GIVTDRDLRQSAPSPATTLEVHELNYLLERITIEAIMTKNVITVTPETGLLEAAKLLL
                     THKIGCLPVVEHEELVGIITEG"
BASE COUNT          373 a          781 c          730 g          510 t
ORIGIN      
        1 gctgttcgct ggtcagcggc tgacgcttaa cgctcatccc tccctgcggg agcagggagg
       61 gatgaggctt ctcgcatgtt cgaaatcttt ggtgttccgt cctcggcgct gtttggtcag
      121 cttctaatcg ggctgatcaa cggctcgttt tatgcgctgc tcagtctcgg gctcgccgtc
      181 atcttcggca tgttgaacat catcaacttc gcccacggcg cccagtacat gatgggggcc
      241 ttcgtggcgt acttcctgtc gaagtacgcg ggcctgggct actggtgggc actgcttctc
      301 gtcccgatcg tcgtcgggct ctttggcatc gccctggaac gcgccatgct ccggcggctg
      361 taccaccttg accacttgta cgggctgctc ctcaccttcg gcctggtcct gatcatccag
      421 ggcctgtttc gcaatgcctt cggatcatcg ggactgccct accgcatccc gaccacgctt
      481 gagggcgggc gcaacctggg cttcatgttc ctgcccaact atcgcgcctg ggtcgtcgtc
      541 ttctcgctgg ctgtgtgctt ggccacctgg tttgtgatcg agcggaccaa gctcgggtcc
      601 tacctccgcg ccgccacgga aaacccggat ctggtgcagg ccttcggcgt gaatgtcccc
      661 cggatgatca ccctgaccta cggaatcggc gtggggctgg cggcactggc cggcgtcttg
      721 gcggcgccca tctaccaggt gaacccgctc atgggcgccg atctcatcat cgtggtcttc
      781 gccgtggtgg tgatcggcgg gatggggtcc atcctgggct ccatcatcac ggggtttgcc
      841 ctgggggtga tcgagggcct caccaaggtc ttctatcccg aggcgtccaa caccgtgatc
      901 tttgtcctca tggccgtcgt gctcctctgg aggccggcag gactcttcgg aaggacatcc
      961 tgacgtgacc acggcacggg agtacctgga agctccggcg aaacagggaa tcatcgtgac
     1021 catgacgcca gagcacatcg gagtcggcgt catggtcgcc ctgttcctgg tggcgccttt
     1081 cgtcgtgtac cccgtctttc tcatgaaggc gctgtgcttc gccctgttcg cctgcgcctt
     1141 caatctactc atcgggttcg tgggtctcct ctccttcggc cacgccgcct ttctgggctc
     1201 ggcgggttac gccacggccc atgcggccaa ggtatgggga ttcccgcccg agctggccat
     1261 cctgttcgga accggggtcg ccgccctgct cggcctcatc atcgggtccg tcgccatccg
     1321 ccggcaaggg atctacttcg cgatggtcac gctatccctg gctcagatga tctacttctt
     1381 ctccgtccag gcgcccttca ctgggggcga ggacggcatc cagggcgtgc cgcgcggaag
     1441 aatgctcggg gtcctggatc tgcagaacac gctgaccctc tacttcgtcg tgctgggaat
     1501 cttcctgggc gggttcctgc tgatctaccg gaccatccac tcccccatgg gtcaggtgct
     1561 caaggcgatc cgcgaaagcg agcagcgcgc aatttcactc ggctacaatc cggaccacta
     1621 taaactgatc gccttcgtgc tgtccgcaac gctgtcgggg ttggcaggcg gcaccaagtc
     1681 catcgtcttc cagcttgcat cactcaccga cgtgcactgg agcatatcgg gcgaggtggt
     1741 cctgatgacg ctcttgggcg gcctgggcac ggtcttcggg ccggtggcag gtgccctcat
     1801 cgtcgtcacc ttggagaact atctggtcca actcggcgcc tgggtgacgg ttgtccaggg
     1861 cgccatcttc gtcgtctgcg tcctggtatt ccgccgcggc gtcgtcgggg agctcggacg
     1921 gttcgtcagg aaggtgcaaa acccggcctc agccaatggg tgacctcgcc agaagccgga
     1981 ctcgtccgga agccgatcca cgcctccggc cggaggagga atcatgctag tcaaagagcg
     2041 gatgaagcgc gatcccgtca ctgccaagaa agacgacagc ttccgctacg ccctgaagct
     2101 cctccggaag gaggggatcc ggcatctccc tgtcttggac gggaaacggc tggtcggcat
     2161 cgtcaccgac cgggacctcc gccagtcggc cccctccccc gccaccactc tggaagtcca
     2221 cgagttgaac tacctcctgg agcggatcac gatcgaggcc atcatgacca agaacgtgat
     2281 cacggtaacc ccggagaccg gcctcctgga ggcggccaag ctcttgttga cccacaagat
     2341 cggctgcctg ccggtggtgg aacacgagga gctggtggga atcatcaccg aggg
//
LOCUS       DBNGZC010000192         4386 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_406836, whole genome shotgun sequence.
ACCESSION   DBNGZC010000192 DBNGZC010000000
VERSION     DBNGZC010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_406836"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="AC16G0_03550"
     CDS             complement(<1..195)
                     /locus_tag="AC16G0_03550"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR5098729.1"
                     /translation="MRNLLSAIRGAVDRVIRWIIIVLMLVMTVTVFLQIVFRYVFNIP
                     LAWSEEAARFSFAWVSFFGAS"
     gene            complement(270..1298)
                     /locus_tag="AC16G0_03555"
     CDS             complement(270..1298)
                     /locus_tag="AC16G0_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018303396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR5098730.1"
                     /translation="MKSRSQGMTRRDLLKAGAVAGVGLGLPAGLWRPARAAAAKIVIK
                     LGSGVAPDHPENIGGRKIKELVEKKVGDRVEVQLFTDNQLGDQRTMVENMRNGTLEVT
                     WVTVGFFGSYEPILNVIESGYLFRDSWHSYKVFDGPFGEEIRGHVEKHGVKLLGYWEA
                     GLRHITNSVRPIRTPEDLKGLKIRTPQAKYHLNTLKYMGANPVAMSFGELYTAMQQKV
                     VDGQENPVSNIYKSKFYEVQKYISLTGHLHLTHMVMYSAKLWDKLPPDLQKVVRESVI
                     ESQETQRKAVRDDDATLLQELKAKGMQANEADREAFRKTVLPLRDEAVKEFGPKAKEW
                     IERIEATK"
     gene            complement(1404..2141)
                     /locus_tag="AC16G0_03560"
     CDS             complement(1404..2141)
                     /locus_tag="AC16G0_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HIR5098731.1"
                     /translation="MTHWTITMLDTGTQTQDKGGMTYLTDMGQTIHIPVTVALIEGPK
                     KILVDTSFLSVERTWQIRRRKITRGPTQELAAALAAAGARSEEISSVILTHLHYDHAG
                     NTRLFPKARFFVQREELRYALAPTAFDATAYFAPSLGITPDFLGTKFDLLDGDAEIED
                     GVRVITTPGHTPGHQSVLVDTAAGCYCVAGDAVMWYENLERNIPPGIHTSTIDCMESM
                     ARIAREAEHVLPGHDPKLFQRTPARFP"
     gene            complement(2183..2995)
                     /locus_tag="AC16G0_03565"
     CDS             complement(2183..2995)
                     /locus_tag="AC16G0_03565"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5098732.1"
                     /translation="MDLGLKGKVAVVTGGARGIGRGIALTLAREGVRVAVNYVSRTDA
                     AEEVVRRIREGGGEAQAIQADVGIYADAERLVTEALQRFGRIDILVNNAGIVSRKPIL
                     EVPLDEWDRVVHTNFYGCFHCSRLVGRHMLARGGGGKIVSISSIHGRVAKASLGPYCA
                     TKAAIDMFSKQLAVELAPHRINVNVVASGTIITEINLPLYQSTKPEDIEKRQAVLHRV
                     PWGEIGQPEDIGGAVAFLVSDAARYITGAVLYVDGGYTAEGTPRMSSGAQVP"
     gene            complement(3152..4042)
                     /locus_tag="AC16G0_03570"
     CDS             complement(3152..4042)
                     /locus_tag="AC16G0_03570"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5098733.1"
                     /translation="MGQTVAFIGMGLMGRRMAKNVLKAGHALRVYNRTSAKSEELRKL
                     GATVAGSPRDAAQGADAVITMVADPPALRAVLAGVDGAFAGCRQGALVIDMSTVDPET
                     SRGMAAEAQARGLRYLEAPVTGGLGAAEQGTLLIMAGGRMEDFQAAKPLLETMGKKVL
                     RVGQMGSGAAMKLTTNMVASSISAAMAEALVFSAKAGLEPILVAEILAERSPLIARWA
                     PRVLAGDFSPFFPLRLAHKDVHLALAAARGLGVPLFSLAAVGQLQTAALAKGLGDLDQ
                     TATIQVLEEIVGLPVRQPKV"
     gene            complement(4099..>4386)
                     /locus_tag="AC16G0_03575"
     CDS             complement(4099..>4386)
                     /locus_tag="AC16G0_03575"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR5098734.1"
                     /translation="TAILVILTPVLMDLITKVGIDPVHFGVLFTLNLMIGLLTPPVGM
                     VLYVMVSLAKVSVTEFTKECGIFMLALLVVLILITYVPGLVTFLPQLIMGS"
BASE COUNT          741 a         1513 c         1314 g          818 t
ORIGIN      
        1 actcgccccg aaaaacgaca cccaggcaaa ggagaagcgc gccgcctctt ccgaccaggc
       61 gagggggatg ttgaaaacgt atcgaaagac aatctgcaag aaaaccgtca ccgtcatgac
      121 cagcatcagg acgatgatga tccagcggat caccctgtca accgcacctc ggatcgccga
      181 gagaaggttc cgcatgccct cactcccaat gggtcattac ggcaggcgcg ggagcagttc
      241 cgccgcgcct gccgcagccc gctccagcgc tatttcgtgg cctctattcg ctcgatccac
      301 tccttggcct tgggaccgaa ctccttcacg gcctcgtccc ggaggggcaa gacagtcttg
      361 cggaaggcct cacggtcagc ctcattcgcc tgcattccct ttgcctttag ctcttgcagg
      421 agcgtcgcgt cgtcgtcccg gactgctttc cgctgcgtct cctgggattc gatcacggat
      481 tcccgcacga ccttctgaag gtcgggaggc agcttgtccc acagcttggc gctgtacatc
      541 accatgtggg tcaggtgcag gtgaccggtg agggagatat acttctgcac ctcgtagaac
      601 ttggacttgt agatgttgga aaccgggttc tcctggccat ctaccacctt ctgctgcatg
      661 gcggtataca gctcgccgaa ggacatggcc accgggttgg cccccatgta cttcagggtg
      721 ttcaggtggt acttggcctg gggtgtccga attttcagcc ccttcaggtc ttcgggcgtc
      781 ctgatgggcc gaacgctatt ggtgatgtgc cggaggccgg cctcccagta gccgagaagc
      841 ttcaccccgt gcttctcgac atggccccga atctcctcgc caaagggacc gtcgaacacc
      901 ttgtaggaat gccagctatc ccggaacaga tagccgctct caatgacgtt caggatgggc
      961 tcataggacc cgaagaagcc cacggtgacc caggtgacct ccagcgtgcc gttccgcatg
     1021 ttctcaacca tggtccgctg gtcgccgagt tggttatcgg taaagagttg gacctccacg
     1081 cggtccccga ccttcttctc caccagctcc ttgatcttcc gacccccgat gttttccggg
     1141 tgatccggtg caacccccga gccgagcttg atgacgatct tggccgctgc tgcccgggca
     1201 ggacgccaga gacccgcggg cagtcccagg cccaccccgg cgacggcacc cgccttcaag
     1261 agatcgcgcc gcgtcatgcc ttggcttctg gacttcatgc tgcgctcctt tccctccaag
     1321 aggtgtcccg tgaaatattg cgctgcctac cgtcctgcgt tttccgccgc ctccgtctca
     1381 cctcctcacc gcccccggat ccgtcagggg aaccgcgcgg gcgtgcgctg gaacagcttc
     1441 ggatcgtggc cggggaggac gtgctccgcc tcccgggcga tccgggccat ggactccatg
     1501 cagtcgatcg ttgaggtgtg gattcctggg ggaatgttgc gctccaggtt ctcgtaccac
     1561 atcaccgcat cgccggcgac gcaataacag cccgctgccg tatccaccag caccgactgg
     1621 tggcccgggg tgtggccggg ggtggtgatg actcgcacgc cgtcctcgat ctcggcatcc
     1681 ccgtccagca aatcgaactt tgtccccagg aagtccgggg tgatccccag gctgggcgca
     1741 aagtaggcag tggcgtcaaa ggcagtcggc gccagggcat accgcaattc ctcccgctgg
     1801 acgaagaacc gagccttcgg gaataaccga gtgttccctg catggtcgta atgcaggtgc
     1861 gtcaggatga cgctggagat ctcctccgag cgagcgcccg cagcggccag cgccgccgcc
     1921 agttcctgcg tcgggcctcg cgtgatcttc cggcggcgaa tctgccaggt gcgctccacc
     1981 gagaggaagc tggtatccac caggatcttc ttcggacctt cgatcaacgc cacggtcacc
     2041 gggatgtgga tcgtctggcc catgtcggtg aggtaggtca tccccccctt atcctgggtc
     2101 tgggttcccg tgtccagcat cgtgatggtc cagtgtgtca tcctgtcctg tctcaagatc
     2161 gtgaggtgag aaggttcaag ccttacggaa cttgcgcccc ggatgacatt cgcggcgtcc
     2221 cctccgccgt gtaccccccg tccacgtaca gcacggctcc ggtgatgtac cgggcagcat
     2281 cggagaccag gaaggcgacg gcacccccga tgtcttcggg ctgcccgatc tcgccccagg
     2341 ggacgcggtg gagtactgcc tggcgcttct cgatgtcctc gggcttggtg ctctgataga
     2401 ggggaagatt gatctccgtg atgatcgtcc ccgaggccac cacgttcacg ttgatccggt
     2461 ggggcgccaa ctccaccgcg agctgcttgc tgaacatgtc gatggcggcc ttggtggcgc
     2521 agtacgggcc caggctggcc ttcgcgaccc ggccatggat ggaggagatg ctgacgatct
     2581 tccccccgcc gccccgcgcc agcatgtgcc ggcccaccag gcgcgagcaa tggaaacagc
     2641 cgtagaagtt cgtgtggacc acgcggtccc actcgtccag cgggacctcc aggatcggct
     2701 tgcgactcac gatccccgcg ttgttcacca ggatgtcgat ccggccaaag cgctgcaggg
     2761 cctccgtcac caggcgctcc gcatcggcgt agatcccgac atccgcctgg atcgcctgcg
     2821 cctcgcctcc gccttcccgg atgcgccgga ccacctcctc ggccgcgtcc gtccggctca
     2881 cgtaattcac ggcgacccgc acgccttccc gggccaacgt cagtgcgatt ccccgcccga
     2941 tgccgcgggc accgccggtg acgaccgcga cctttccctt gaggccgaga tccatggctt
     3001 tccctcaccc agttgcggcc ggctcgagcg ggcacgtccg gatttccgat ccactgcggg
     3061 atgggaacac tctcataact cctcgtgtgt gtgagagtca acacgattca agaagtggca
     3121 atctgatctg tcgccgccca tcgctcccca gtcagacctt gggctgtcgc accgggaggc
     3181 cgacgatctc ctccagtacc tggatcgtcg ccgtctggtc gagatcaccc agcccctttg
     3241 ccagggcggc cgtctggagc tgcccgaccg cggccaggct gaagagggga acgcccagcc
     3301 cgcgggcggc ggccaacgcc aggtggacgt ctttgtgcgc cagtcggagc gggaagaacg
     3361 ggctgaagtc gcccgccagg acccgagggg cccagcgcgc tataaggggc gagcgttctg
     3421 ccagtatttc ggcgaccagt attggctcca gaccggcctt ggcggaaaac accagcgcct
     3481 cggccatggc ggccgagatg ctcgaagcca ccatgttggt ggtgagcttc atggcggccc
     3541 cgcttcccat ctggcccacg cgcagtacct tcttgcccat ggtctcgagg agcggcttcg
     3601 ccgcctggaa atcttccatc ctaccaccgg ccatgatgag gagggtcccc tgctccgccg
     3661 cccccaggcc gccggtgacg ggcgcctcga ggtagcggag tccccgagcc tgcgcctcgg
     3721 cagccatccc ccggctcgtc tccgggtcca ccgtgctcat gtcgatcacc agcgccccct
     3781 gccgacagcc cgcgaaggcg ccatcgactc ccgccagcac ggctcggagc gctggcgggt
     3841 ccgccaccat ggtgatcacg gcatcggccc cctgggccgc gtcccgcggg gagcccgcga
     3901 ccgtcgcccc cagcttccgc agttcctcgc tcttggccga tgtccggttg tacacccgca
     3961 gggcatgacc ggccttcagc acattcttgg ccatccgcct gcccataagg cccatgccga
     4021 taaaggccac cgtctgtccc atggctgctt atccctcccc cggtctctgg gtgtcgcgcg
     4081 agccgggcgc cccggagatc aacttcccat gatcagttgc ggcaggaacg tcacaaggcc
     4141 cggcacgtag gtgatcagga tcaggaccac caacagcgcc agcatgaaga tcccgcactc
     4201 tttggtgaac tcggtgacgg agaccttggc caggctcacc atgacgtaga gcaccatccc
     4261 caccggcggc gtgagcaggc cgatcatcag gttcagggtg aacaacacgc cgaagtggac
     4321 cggatcaatg cccaccttgg tgatcaggtc catgaggacc ggcgtcagga tcaccaggat
     4381 ggccgt
//
LOCUS       DBNGZC010000193         1201 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_896159, whole genome shotgun sequence.
ACCESSION   DBNGZC010000193 DBNGZC010000000
VERSION     DBNGZC010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1201)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_896159"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /gene="pstC"
                     /locus_tag="AC16G0_03580"
     CDS             <1..874
                     /gene="pstC"
                     /locus_tag="AC16G0_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR5098735.1"
                     /translation="ALSLLLTVAAMVLMLGRAAWPSITAFGWRFLITSTWDPVAEHFG
                     ALPLIYGTLISSLLALVIAVPLGIGAAIYLSEVAPVWIRPVVCFLVELLAAVPSVVYG
                     LWGIAVMAPFLRTYVQPALGATLGFLPLFQGPPYGVGMLAAGIILAIMIVPFIIAVSR
                     EVLQAVPNTQREAALALGATRWETTRLAVLRYGKSGLIGAVLLGLGRALGETMAVTML
                     IGNRPEIAVSLFAPGYTMASMLANEFTEATSDLYLSALVEVGLLLFIVTVVVNALARL
                     LVWSVGGAVKDVRE"
     gene            907..>1201
                     /locus_tag="AC16G0_03585"
     CDS             907..>1201
                     /locus_tag="AC16G0_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR5098736.1"
                     /translation="MGGLTAGAAFVVILPLILILAFLVHQGASALNLQFFTHLPKPVG
                     EPGGGMDNAIVGTVILLGLATCLGLPLGILGGVYLSESQDRRLPWLVRFLADVL"
BASE COUNT          144 a          406 c          379 g          272 t
ORIGIN      
        1 cgccctgtcc ttgctgctca cagtggcggc catggtcctc atgcttgggc gggccgcctg
       61 gccatccatc acagccttcg gatggcgatt cctgattacc tccacctggg atcccgtggc
      121 cgagcacttc ggggcgttgc cgctcatcta cgggacgctc atctcgtccc tgctcgcctt
      181 ggtgatcgcc gtgcccctgg ggatcggcgc cgcgatctat ctctcggagg tggcccccgt
      241 ttggatccgc ccagttgtat gcttcctggt tgagctgttg gccgcggtcc cgagtgtggt
      301 ctacgggctc tgggggatcg ccgtcatggc tcccttcctg cggacgtatg tccagccggc
      361 cctcggcgcc acactggggt tcttgccatt gttccaaggt cccccctacg gggtggggat
      421 gctcgccgcc gggatcatcc tggcgatcat gatcgtcccc ttcatcattg ccgtgagccg
      481 cgaggtcctc caggcagttc cgaacaccca gcgggaggcg gccctggcgc tgggggccac
      541 ccgttgggag accacccgac tcgccgtcct gcgctacggg aaatccggtc tcatcggcgc
      601 cgtcctgctc ggcctcggcc gtgcccttgg ggaaactatg gcggtcacca tgctcatcgg
      661 caaccggccg gagatcgccg tgtcgctctt cgcgccgggc tacaccatgg ccagcatgct
      721 cgccaatgag tttacggagg ccacctccga cctgtacctg tccgccctgg tcgaggtcgg
      781 gctcttgctc ttcatcgtga cggtcgtcgt gaatgctctg gcccggctcc tggtttggag
      841 cgtgggcggg gcggtgaaag atgtgcggga atgagggcgg agttccggcg catcctcaat
      901 cttctgatgg gtggtctcac ggccggcgcc gccttcgtgg ttatcctccc cctgattttg
      961 atcctggcct ttctcgtgca tcagggtgcc tcggcgctca atctccagtt cttcacccac
     1021 ctgccgaagc cggtgggaga gccgggtggc gggatggaca acgccattgt cgggaccgtg
     1081 atccttctgg gcttggccac ctgcctcggg ctccccctag gcatcctcgg cggtgtctac
     1141 ctgtcggaat cgcaggaccg tcgacttccc tggcttgtcc gctttctggc tgatgtcctg
     1201 a
//
LOCUS       DBNGZC010000194         5818 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_994316, whole genome shotgun sequence.
ACCESSION   DBNGZC010000194 DBNGZC010000000
VERSION     DBNGZC010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_994316"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1091)
                     /gene="nagZ"
                     /locus_tag="AC16G0_03590"
     CDS             complement(9..1091)
                     /gene="nagZ"
                     /locus_tag="AC16G0_03590"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HIR5098737.1"
                     /translation="MRRLVLPLVCVGFDGPNLPDGLRPLLAEGLGGVILFRRNLQELE
                     QICRLTAELHAAASAPLLVGVDQEGGRVTRLPDPFLAPPPAATLGDLNDETLTVDLAR
                     AVGRELLSAGFTWNLAPVLDVHSNPANPVIGDRAFSHDPDCVARLGVWVIQGLSQAGV
                     LPTAKHFPGHGETIADSHLTLPISSQPRERWRSVEFVPFRRAILAGVPMVMVAHLDCP
                     ALDPAAPSSLSHAVITEILRKELGFAGVVVSDDLEMEAIAARFDIGEAAVRFLEAGGD
                     LILICHEAARQEAAVTAVEAALRSGRLTETRLAESLDRIDRLRRRLPTKGDQLDVSAA
                     RTIVGTSAHRRLLQAALERRDRTNAS"
     gene            complement(1091..1855)
                     /locus_tag="AC16G0_03595"
     CDS             complement(1091..1855)
                     /locus_tag="AC16G0_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098738.1"
                     /translation="MSCNSATPDPLPPVRRRGRLCSSAVLLLAMLAGPAHGAFTLIGL
                     TVEDAVARQVVERYTIARQLQPVRFLGSLRTTEWLLDRPPLAATLARHLHPPLERYHV
                     REKGDGVYTVDDQGALRGEFRLVARGLDRRVYLVEGQFRSLAYILSLSGSMVFTLEYR
                     ETRQGNESYVEVTPQLFVRLNNVAAHAILKVLAPLIHGVIDRRVANLTAAAEVVGQRV
                     AKDPIGLYREMRTWPDIRPEEVEAFRLAFSNGEGGQ"
     gene            1963..3258
                     /locus_tag="AC16G0_03600"
     CDS             1963..3258
                     /locus_tag="AC16G0_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5098739.1"
                     /translation="MRTNTKFFWVAVLYFAEGFPFGVVKDALPVFFRVHGVRLADIGL
                     LILVGLPWSLKFLWAPAVDLWGQRRTWILGCQGLLVLGILALLIVDPSRLTPALWALL
                     LGLAILSATQDIAIDAYTIELLDEAEMGPANGLRVTTYRIALISAGGMLVALAGVIGW
                     RAAFVAAAILMGGVVLLSWRMPHPEEPPAAVVRLGRGVAGFQDAVWSPLQHFFAHPGF
                     VFVLCFILLYKLGDMSLGPMVRPFWVDRHFSMLEIGAVPGTLGVLSTILGALTGGSLT
                     KRWGILRALWLLGIAQAASNLMYAAAAALPPSTVLMYAASIVESFCGGLGTAPFLAYL
                     MSICDKAHAATQYALLSALFAPLGLVAGMVSGWAVERLGYAAYFTVTFFLAWPALLLI
                     PWVRRWTVARTAALHPSVPQADPGASDTASPPKKGALPG"
     gene            complement(3233..4531)
                     /locus_tag="AC16G0_03605"
     CDS             complement(3233..4531)
                     /locus_tag="AC16G0_03605"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019307834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR5098740.1"
                     /translation="MWLIAKRVGLIDTENAFKVGPYIRKIEEQGKKVIKCNLGEPDFP
                     VPQFIKEEVKRQIDLDNTHYCDPQGILPLRKAIAKQLSETRGIQATPERVVVFPGAKP
                     PIGFSQQTYCDPGDEVVYPSPGFPIYESFIAYVEAKPVPAHLQEEKGFSLSGKELSSL
                     LTRQSKLVFLNFPSNPTGGVASKEQLQDIAEVIRDRCSDEVRVFSDEVYENILFDGSQ
                     HHSIVSFPGMEKITILVSGASKSFSWTGGRIGWALFPTAQEADLFRNLNINYFSCIPA
                     YNQEGARLAVESPQSRQAIQEMVRVFQQRRDLVVEGLNAIEGVRCQTPKGAFYVFPNI
                     AGVCEKLGIMEAYRRIPPKVQERTSPSTLFQMFLLFEYQVATLDRKSFGRIGTEHLHY
                     LRISIATETESLREGVKRFAAAAQDRKGFSQFLSRGEHLF"
     gene            complement(4687..5091)
                     /locus_tag="AC16G0_03610"
     CDS             complement(4687..5091)
                     /locus_tag="AC16G0_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5098741.1"
                     /translation="MAAPTQPPQATILVIDDQPFFLTMLQNILQAKGYRVVVANGGDE
                     GLAQARKHKPDAILLDVEMPGKDGFTVCQDLKQDEALKHIPVIILTATQNTKLNEKAF
                     QAGASITAMKSMAGDRLLNTLSLTLKQPKSSA"
     gene            complement(5245..>5818)
                     /locus_tag="AC16G0_03615"
     CDS             complement(5245..>5818)
                     /locus_tag="AC16G0_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098742.1"
                     /translation="HGPCDAFKVIRDIPMIARRSRRSREEVRGLLGVNGTRPVVLLSF
                     GGFEVQGIDFDRVETLHDYCFLVTQPPPRPVRNVRVVTLGALKYEDVVAQADVVLTKP
                     GYGIVSDCLANRTPVLYTSRGEFAEYAPLVKGLERFGIAAFMENRDLLSGNWRSGLEA
                     LLQRSRTWADLPANGAGVAADILQALVPRI"
BASE COUNT          972 a         1884 c         1871 g         1091 t
ORIGIN      
        1 gggcgcgatc aagaagcgtt ggtccggtct cggcgttcaa gagcggcttg gaggagccgc
       61 cgatgagcag atgttcccac gatggtgcgg gcagcagaca catccaattg gtcgcccttt
      121 gttggcaggc ggcgacgcag gcgatctatc cgatccaggc tttcggccag tcgcgtttcg
      181 gtcagtctgc cgcttctcag cgccgcctcc accgcagtca cggccgcctc ctgccgggct
      241 gcctcgtggc aaatgaggat caggtcgcca ccagcctcca ggaagcggac ggcggcctcg
      301 ccgatatcga agcgggccgc gatcgcctcc atttctaggt cgtcactgac gacgacgcca
      361 gcgaaaccca attccttccg gagaatctca gtaatgaccg cgtgggatag gctgctgggt
      421 gcggccgggt ccagagccgg gcagtccagg tgcgccacca taaccatggg cacgccggcc
      481 aagatcgccc ggcggaaggg cacgaactcc acacttcgcc aacgttctct tggctgcgag
      541 ctgatcggga gcgtcagatg ggagtctgca atggtctcgc catgacccgg gaagtgcttg
      601 gccgtcggca ggacgcctgc ctgggaaagg ccctgaataa cccagactcc gaggcgggcg
      661 acgcaatcgg ggtcgtgact gaaggcccgg tcgccgatca ccgggttcgc cggattcgag
      721 tgcacgtcca gtacgggcgc gaggttccag gtgaagcctg ccgacaatag ttcccgcccc
      781 acggccctgg ccaggtccac tgtgagagtc tcatcgttca agtcccccag tgtggccgcc
      841 ggcggcggag cgaggaacgg gtcgggaagc cgcgtgacgc gtcccccctc ctgatccacg
      901 cccaccagga gcggcgccga ggccgcggcg tgaagttcgg ccgtcaggcg gcagatctgc
      961 tccaactcct gaaggttgcg gcgaaagagg atgacgccac ccagtccctc ggccaacaag
     1021 ggtcggaggc cgtcaggcag atttgggccg tcgaagccga cgcagacaag cggcaggaca
     1081 aggcgccgca tcattgcccc ccctcgccgt tggagaaggc gaggcgaaag gcttcgacct
     1141 cctccggccg aatgtcgggc catgttcgca tttcgcggta gagcccgatc ggatccttgg
     1201 ccacacgctg gccaacgacc tcggcagccg ccgtgagatt cgccacgcgg cggtcgatga
     1261 ccccgtggat gaggggtgcc aggaccttca ggatggcatg ggccgcgacg ttgttcaggc
     1321 ggacaaaaag ctgcggcgtg acctcgacat aggactcgtt gccctgccgt gtctcccggt
     1381 attccagagt gaagaccatg cttcccgaca ggctcaggat gtaggcaagg gagcggaact
     1441 ggccctcgac gagatatacc cggcggtcca gaccgcgggc aaccagccgg aactccccgc
     1501 gaagcgcccc ttggtcatcc accgtataga cgccatcccc cttttccctg acgtgatacc
     1561 gttccagcgg cggatggaga tgccgggcca gcgtggctgc gagcggtggt cgatccagca
     1621 gccattcagt agtcctgagg ctgccaagga agcgcaccgg ctgcagttgc cgcgcgatgg
     1681 tatagcgctc cacgacctgg cgcgcgactg catcctcgac ggtgagaccg atgagcgtga
     1741 aggccccgtg ggccgggccg gccagcatcg ccaagagcag gacggcgctc gagcacaatc
     1801 ggccccgtcg ccgaacaggc ggcaggggat cgggcgtcgc ggagttacaa ctcatcggga
     1861 atttgacagc ccggcggcgc aggcattata aggtaccggc gtccgctccg ggacctgata
     1921 gtgcgcgttc cggccgaatt ctcccacggg aagagagggc atgtgaggac caacaccaag
     1981 ttcttctggg ttgccgtgct gtattttgcc gagggatttc cctttggcgt cgtcaaagat
     2041 gccctccctg tcttcttccg ggttcacggc gtccgcctgg cggacatcgg gctcctgatc
     2101 ctggtgggac ttccctggtc tctcaagttc ctgtgggctc ctgcggtgga cctctggggg
     2161 caacgccgca cctggatact gggatgtcag ggcctgctgg tcctcggcat cttggctctg
     2221 ctgatagttg acccgtcgcg gttgacgccg gccttatggg cgcttctctt ggggttggcg
     2281 atcctgtccg ccacccagga tatcgccatc gacgcttaca ccatcgagct tctggacgag
     2341 gccgaaatgg ggcctgccaa cggcctccgg gtgaccacgt accgaatcgc gctcatctcg
     2401 gccggcggca tgctggtcgc gctggcggga gttatcggct ggagggcagc ctttgttgct
     2461 gcggccatcc tcatgggagg tgtggtcctg ctctcctggc ggatgcctca cccagaagag
     2521 cctcccgccg ccgtggtgag gctcggtcgc ggagtcgctg ggttccagga cgccgtctgg
     2581 tccccgctcc aacacttctt cgcgcacccc ggcttcgtct ttgtcctctg tttcatcctg
     2641 ctctacaagc tgggagacat gtccctcggg cccatggtgc gtcctttctg ggtcgatcgg
     2701 cacttctcca tgctcgaaat cggcgcggtc cctggcaccc tgggagtcct aagcacgatt
     2761 ctgggcgcgc tcacgggcgg gagcctcacg aagcgttggg ggatcctccg tgccctgtgg
     2821 ctgctgggaa tcgcccaggc agcctcgaat ctcatgtacg ccgcggctgc cgccctcccc
     2881 ccctccacgg tcttgatgta cgccgcgtcc atcgtggagt ccttctgcgg cggcctgggg
     2941 acggccccct tcctggccta tctgatgagc atctgcgaca aggcccacgc agcgacccag
     3001 tatgccctcc tgagcgccct cttcgccccc ctcggcctgg tcgccgggat ggtttcgggc
     3061 tgggccgtgg agcgtctggg ttacgccgcc tatttcaccg tgacgttctt cctcgcctgg
     3121 ccggccctct tgctgatccc ctgggttcgc cgctggacag tcgcgcgaac cgcagcccta
     3181 cacccctcgg tgccgcaggc tgatcccggc gccagcgaca cggcatcgcc gcctaaaaaa
     3241 ggtgctctcc ccggctgagg aactggctga atcccttcct gtcctgggcg gcagccgcga
     3301 agcgcttcac gccctccctc agggactcgg tctccgtggc gatggagatc cgcaggtagt
     3361 gcaggtgctc ggttccgatc cgcccgaagg atttccggtc cagggtcgcc acctgatact
     3421 cgaacagcag gaacatctgg aacagggtgg aagggctcgt ccgttcctgc accttcgggg
     3481 ggatcctccg gtaggcctcc atgatcccca gcttctcaca tacccccgcg atgttcggga
     3541 agacgtagaa ggctcccttc ggcgtctggc accggacccc ctcgatggcg ttcagcccct
     3601 ccacgacgag gtcccgtcgt tgctggaaga cgcggaccat ctcctggatg gcctggcggc
     3661 tttggggcga ttccaccgcg aggcgcgccc cctcctggtt gtaagcgggg atgcaggaga
     3721 agtagttgat gttcagattc cggaagaggt cggcttcctg tgcggtcggg aagagggccc
     3781 agccgattcg gcccccggtc caggaaaagc tcttcgaggc gccgctgacc aggatcgtga
     3841 tcttctccat tccagggaag gagacgatgc tgtggtgctg gctgccgtcg aagaggatgt
     3901 tttcgtaaac ctcgtccgag aacacccgga cctcatccga acaccgatcg cggatgacct
     3961 cggcgatatc ctggagctgc tccttggagg cgacccctcc cgtcggattc gacgggaagt
     4021 tcaagaagac cagcttggac tgcctggtca ggagcgaaga cagttccttc cccgagaggg
     4081 agaagccctt ctcctcctgc aggtgagccg ggacgggttt cgcctcgacg taggcgatga
     4141 aggactcgta gatggggaat cccgggctgg gatagaccac ctcatctccc ggatcgcaat
     4201 aggtttgctg gctgaagccg atggggggct tggcgccggg aaaaacaacg acccgctccg
     4261 gcgtggcctg aatgccccgg gtctcactga gttgcttggc gatggccttc cggagcggca
     4321 gaatcccctg ggggtcgcag taatgggtgt tgtccagatc gatctgccgt ttgacctctt
     4381 ccttgatgaa ttgcgggacg gggaagtccg gctcgcccag attgcacttg ataaccttct
     4441 tgccctgttc ctcgatcttc cggatgtacg gtcctacctt gaaggcattc tcggtgtcga
     4501 tgagcccgac tcttttggca atgagccaca cggtgacctc ctttcctcct catcggagga
     4561 gggcggcggc cagtcattcc gccgaccggc tgccgccgag gggagcgttg gtgaaatcga
     4621 gcgggccgaa ggccaagacg atgaaagcag ccgcgacgct tcgaaggcgg cgccgccatc
     4681 cgcgtttcac gccgaggact tgggctgctt gagcgtcagg ctgagggtat tgagcaatcg
     4741 gtccccggcc atgctcttca tggccgtgat ggacgcaccc gcctgaaagg ccttctcgtt
     4801 cagcttggtg ttctgcgtcg ccgtcaggat gatcaccggg atgtgcttga gggcttcatc
     4861 ctgcttcagg tcctggcaca ccgtgaagcc atctttcccc ggcatctcca cgtccaggag
     4921 gatggcgtcc ggcttgtgct tccgggcttg ggccaggcct tcgtcgccgc cgttcgcgac
     4981 aaccacccgg tagcccttgg cctgaaggat gttttgcagc atggtgagga aaaaaggctg
     5041 atcgtcgatc accaggatcg tcgcctgtgg gggttgcgtc ggcgcggcca ttccctcggc
     5101 tccttgtcgt gaagggtgaa gatttcacgg cggcatgacg accctgcgtc gccgccgcgc
     5161 acgggcaaca ggtcgggcca gcctgcatgc tgagcagatc cgggtgatcg tatccccccc
     5221 cgacgcctca caggccggtc cgggtcaaat gcgaggcacg agagcttgga ggatgtcggc
     5281 ggccacccct gctccgttgg cagggagatc cgcccaagtt ctggaccgct gaaggagtgc
     5341 ctccaagccg ctccgccagt tgcccgacaa aagatcccgg ttctccatga atgccgcgat
     5401 cccgaaccgc tccaggcctt tcacgagagg cgcgtactcg gcaaactcgc cacgcgatgt
     5461 gtacagaacg ggtgtccggt tcgccaagca gtccgacacg atcccgtagc ctggcttggt
     5521 gagcacgacg tccgcctggg ccaccacgtc ctcgtacttg agcgcgccca gcgtcaccac
     5581 ccggacgttc cggacaggtc gcggcggcgg ctgcgtgacg aggaaacagt agtcatgcag
     5641 cgtctccacc cggtcgaagt cgatgccctg gacctcgaag ccgccgaagg atagcaggac
     5701 caccggacgg gtaccgttca caccgaggag acctcgcacc tcctcccgtg accgtcggga
     5761 gcggcgggcg atcatgggga tatcccggat cactttgaag gcgtcgcagg gaccgtgg
//
LOCUS       DBNGZC010000195         1704 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_798671, whole genome shotgun sequence.
ACCESSION   DBNGZC010000195 DBNGZC010000000
VERSION     DBNGZC010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_798671"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="AC16G0_03620"
     CDS             <1..265
                     /locus_tag="AC16G0_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIR5098743.1"
                     /translation="EQGVLCMGPATRSGCGALCLKAGMRCEGCYGPPQNAEDQGTSMI
                     GALGALLDAQSEERARELVAQIADPTGTFYRFSMSSSVMKAAQ"
     gene            282..>1704
                     /locus_tag="AC16G0_03625"
     CDS             282..>1704
                     /locus_tag="AC16G0_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HIR5098744.1"
                     /translation="MTSPHVKTAEGFRRISIDPVTRLEGHGKIDIFLDADGNVADCFF
                     QVPELRGFEKFCEGRPVEELPRITPRICGVCPEAHMMASAKACDAVYAAELPLTAKLL
                     RELQYNIFYVTDHTTHFYALGAPDFVMGPQSDPALRNLIGVIGKVGVEIGGQVIKCRS
                     WGHEAAAIFGGKPVNPTNAIPGGMSKPLKPEERQRLEEICRFMLEFGKFTLKLFHDVV
                     LSNKEYVDLILNGPYYHATYSMGLVDERNRVNFYDGTVRVVDPDGQELCKYAPAEYVQ
                     HIAERVEPWSYLKFPYLKHVGWTGFVDGKASGVYRATPQSRLNVADGMNSPLAQEAYE
                     EYFQTLTGDKSGKKPVHHTLAIHWARVIELLNAAEHALELVRHPEITHPEKYRVVCDK
                     TPTEGVGIVEAPRGTLTHHYVTDERGIVKKANLIVGTTNNHAAICMSIKQAAQGLIKK
                     GVEVTEGVLNMVEMAFRAYDPCFG"
BASE COUNT          336 a          540 c          557 g          271 t
ORIGIN      
        1 ggagcagggc gtcctgtgta tgggacctgc cacccgcagc gggtgcggtg cgctgtgcct
       61 caaggcgggc atgcggtgcg aggggtgcta tggcccgccg cagaacgccg aggatcaggg
      121 cacgtccatg atcggggccc tgggagcctt gctggacgct cagagcgagg aacgggcgcg
      181 ggagctggtc gcacagatcg cagatccaac cgggaccttc taccgtttca gcatgtcctc
      241 cagcgtcatg aaagcggcgc agtgaggagg ggagagccag catgaccagt ccccacgtca
      301 agaccgccga gggattccgc cggatcagca tcgacccggt gaccaggctg gagggccacg
      361 ggaagatcga catttttctc gacgcggacg gcaacgtcgc cgactgcttc ttccaggtcc
      421 cggagcttcg ggggttcgag aagttctgcg agggtcgccc cgtggaggaa ctgccccgca
      481 tcacgccccg catctgtggg gtctgcccgg aggcgcacat gatggcctcc gccaaagcct
      541 gcgacgccgt ctacgccgcc gagctgccgc tcacggcgaa gctcctgcgg gaattgcagt
      601 acaacatatt ctacgtgacc gaccatacca cccatttcta cgccctgggc gcgccggact
      661 tcgtgatggg cccgcagagc gacccggccc tacgcaacct gatcggggtc atcggcaagg
      721 tcggagtgga gatcggtggc caggtgatca agtgccggtc ctggggccac gaagcggccg
      781 ccatcttcgg tggcaagccc gtgaatccca ccaacgccat cccggggggc atgagcaaac
      841 ccctgaagcc cgaagagcgc cagcgtctgg aggagatctg ccgcttcatg ctcgaattcg
      901 ggaaattcac cctgaagctc ttccatgacg tggtgctctc caataaggaa tacgtggacc
      961 tgatcctgaa tggaccctat taccatgcga cctattccat gggcctggtg gatgaacgga
     1021 accgggtgaa cttctacgac ggcacggtgc gcgtggtgga cccggatggt caggagctgt
     1081 gcaagtacgc gcccgccgag tacgtgcaac acatcgccga gcgggtcgag ccctggagct
     1141 acctcaagtt cccctatctg aagcacgtgg gctggacggg gttcgtggat ggcaaggcct
     1201 cgggcgttta ccgcgccacc ccgcaatcca ggctcaacgt ggccgacggc atgaacagcc
     1261 cgctggccca ggaggcctac gaggagtact tccagaccct caccggggac aagagcggca
     1321 agaagccggt ccaccacacc ctggccatcc actgggcgcg cgtgatcgag ttgctgaacg
     1381 cggccgagca cgccctggag ctggtccgcc accccgagat cacccacccc gagaaatacc
     1441 gggtggtctg cgacaagacg cccaccgagg gtgtgggcat cgtggaggct ccccggggta
     1501 ccctcaccca ccactacgtc accgacgagc gagggatcgt gaagaaggcc aacttgatcg
     1561 tgggcaccac caacaaccat gccgccattt gcatgtccat caagcaggca gcccagggac
     1621 tgatcaagaa gggggtggag gtcacggagg gtgtgctgaa catggtggag atggccttcc
     1681 gggcctatga cccgtgcttc ggct
//
LOCUS       DBNGZC010000196         2779 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2656797, whole genome shotgun sequence.
ACCESSION   DBNGZC010000196 DBNGZC010000000
VERSION     DBNGZC010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2656797"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1187)
                     /locus_tag="AC16G0_03630"
     CDS             complement(165..1187)
                     /locus_tag="AC16G0_03630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR5098745.1"
                     /translation="MQITVWGAGAIGGVTGGALTRAGRDVLLVDVDEAHVAAMQKDGL
                     HVSDPRGGWHVPVRAATPGEVRGPLDLVLLAVKAQATAGALEQIFPHLAAGSVVVSLQ
                     NGLNEELIASRIGPARTIGCLVNWSADWIGPGRIQFGGAGSFLVGELDGAATPRLTDL
                     CDLLSDVMPAKTTGNIWGCLWAKVCFGALLFATALADETIYDVVERSPDIQRMLVLLV
                     AEAMDVAETAGVRLEAFDEYDPAWYRAGAKGDRAAITEAMTAVSRFYRNLTKVKSGIW
                     RDLAVRKRKTEVEALLDIVVEKGKGLGVPTPLLIRLIGMIHDLEEGRRSMGWANLDEL
                     AASTLT"
     gene            complement(1242..1931)
                     /gene="rpiA"
                     /locus_tag="AC16G0_03635"
     CDS             complement(1242..1931)
                     /gene="rpiA"
                     /locus_tag="AC16G0_03635"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HIR5098746.1"
                     /translation="MNPKQRAAEYGASLVKSGMVLGLGSGTTSTLAVQAIGRRLREGS
                     LREVRGVPSSSAIATVARESGVPLTTLEEHPVLDLNLDGADEVDPNLNLIKGLGGALL
                     WEKIVATASRQVVILVDDSKLVDRLGSKAPLPVEVIPFGWRTHVSFVESLGGKPGLRT
                     EPDGKPFVTDEGNYILHCRFEAISDPANLEARLLTRAGVVGTGLFLQVAHQVVIGRAD
                     RVEVRSRGGAD"
     gene            complement(2027..>2779)
                     /locus_tag="AC16G0_03640"
     CDS             complement(2027..>2779)
                     /locus_tag="AC16G0_03640"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the reversible formation of D-erythrose
                     4-phosphate and D-fructose 6-phosphate from sedoheptulose
                     7-phosphate and D-glyceraldehyde 3-phosphate; catalyzes
                     isomerization of glucose 6-phosphate and fructose
                     6-phosphate; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HIR5098747.1"
                     /translation="LQDSKDRQLDGKVGALEGAGHPTITILLQDRYDLGAEFFRWEFA
                     TAVSGLVLGVNPFDQPNVQESKDNTRQLLGEFKARGKLPEDDPILVQGPISLYGDQAS
                     AMALRGSRGLADGLRTHLARIKAGDYVALTAYLQATPAHAEALGMIRHHIRDRYRVAT
                     TLGYGPRFLHSTGQLHKGGGDNGVFLQFTADDPVDLPIPGEPHTFGVMKAAQALGDLQ
                     ALQSRKRRVLRIHIAGPIEAGLRRVQEAVGKA"
BASE COUNT          456 a          978 c          857 g          488 t
ORIGIN      
        1 cacgcggtaa ccggcatgcg gttggcctcc tccctgagct ccaaccgcgg ggtagcccgc
       61 cgatccgctc aggatgagcg ccagaacgat ggatgcacag agggaatgtc gcatgcagga
      121 agtcccggct gctgggaaca cgccgcgtgg ttgtctcctg gtcctcaggt gagggtcgag
      181 gccgctagct cgtcgaggtt ggcccagccc atggagcggc gtccctcctc caggtcgtgg
      241 atcatcccga tcagccggat gaggaggggc gtcggcacgc ccagcccttt gcccttctcg
      301 acaacgatgt cgagcagcgc ctcgacttcg gtctttcgct tccgcacggc cagatcgcgc
      361 cagatccccg acttcacctt ggtgagattc cggtagaacc gcgacaccgc cgtcatggcc
      421 tctgtgatgg cagcccgatc accctttgca cccgcacggt accaggcggg atcatactcg
      481 tcgaacgctt cgagtctcac ccccgccgtc tcggccacat ccatggcttc ggccaccaga
      541 aggacaagca tccgctggat gtcaggcgac cgctcgacga cgtcgtagat cgtctcgtcg
      601 gcgagcgccg tggcgaagag aagcgccccg aagcatacct tggcccagag gcacccccag
      661 atgttccccg tcgtcttcgc tggcatcacg tccgagagca ggtcacacag atctgtgaga
      721 cgtggcgtgg ccgcgccgtc gagctccccc accaggaagc ttccagcccc tccgaattgg
      781 atgcggcccg gtccaatcca gtctgccgac cagttcacca agcacccgat ggttctggcc
      841 ggcccgatcc gggaggcgat gagctcctcg ttgaggccgt tctggagcga gacgacgacc
      901 gatccggccg cgagatgcgg aaaaatctgc tctaaagccc cggccgtcgc ctgggccttg
      961 accgccagga ggacgaggtc gagcggcccg cgcacctctc ctggagtggc ggcgcgcacc
     1021 ggaacatgcc agccccctct ggggtcgctc acatgcaggc cgtccttctg catggccgcc
     1081 acatgcgcct catccacatc cacgagaagg acatcccggc cggcgcgggt gagcgcgccc
     1141 cctgtgactc ccccgatggc tcccgcaccc cacaccgtga tttgcatggg aagacctcct
     1201 atcccagatc cggcagacgt gtccaggcgg ggcgctggac ctcagtctgc gcctcctcgg
     1261 gagcggacct ccaccctgtc cgcccggccg atcaccacct gatgggcaac ctgcaggaac
     1321 aggccggtcc cgacgacgcc cgcccgtgtg agcaggcggg cctccagatt ggccgggtct
     1381 gaaatcgcct cgaaccgaca gtgcaggatg tagttgccct cgtccgtcac gaagggcttg
     1441 ccatcgggct cggtccggag cccgggcttc ccgcctagcg attcgacaaa ggacacgtgg
     1501 gtgcgccagc cgaaggggat gacctccacc gggagcgggg ctttgctgcc gaggcgatcc
     1561 acgagcttgg agtcatccac gaggatgacg acctggcgcg aggccgtggc cacgatcttc
     1621 tcccacagga gcgccccacc gaggcccttg atgaggttca ggttcgggtc cacctcgtcc
     1681 gctccgtcca gattgaggtc caggaccgga tgctcctcca gggtcgtcaa gggcaccccc
     1741 gattcccggg cgaccgtcgc gatggcgctg gaggagggaa cgcctcggac ttcacgcagg
     1801 ctcccttcac ggagcctccg gccgatcgcc tggaccgcca aggtcgaggt cgtgccgctc
     1861 cccagaccca ggaccatgcc ggacttcacc aggctcgccc catactccgc cgcccgctgt
     1921 ttcgggttca tgcttgtgat cctttctttg tgtctgatta cgagtcaaat ctgcgcaatc
     1981 tgcgaaatct gcgttaaagg tagatccgct ccttctccgt gcctggctac gctttgccca
     2041 cagcctcttg cacccgtctc agacccgcct cgatgggacc ggcgatatgg attcgcagga
     2101 ctcggcgctt ccggctctgc aacgcctgaa ggtcgccgag ggcctgggcc gctttcatca
     2161 cgccgaaggt gtgcggctca cccggtatgg gcaggtcaac cgggtcgtcc gccgtgaact
     2221 ggaggaagac cccgttgtcc ccgccgccct tgtggagttg ccccgtcgag tgcaggaacc
     2281 gcggcccgta acccaaggtg gtggccaccc tgtaccgatc gcggatgtga tgccggatca
     2341 tcccgagggc ctcggcatgg gccggcgtcg cctgcaggta ggcggtcaac gccacgtaat
     2401 ccccagcctt gatccgagcc aggtgcgtcc ggaggccatc ggccagacct ctcgaccccc
     2461 gcagggccat ggcgctcgcc tggtccccgt agagactgat gggcccctgc accaggatcg
     2521 ggtcgtcctc gggaagcttt cccctcgcct tgaactcccc gaggagctgc ctggtgttgt
     2581 ccttgctttc ctggacgttc ggttggtcga agggattgac ccccaggacc aggccggata
     2641 ccgccgttgc gaactcccag cggaagaact cggcccccag atcatagcgg tcctgcagga
     2701 ggatcgtgat tgtcggatgc ccggcgccct ccagtgcgcc gactttcccg tcgagctgcc
     2761 ggtctttcga atcctggag
//
LOCUS       DBNGZC010000197         4499 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_700055, whole genome shotgun sequence.
ACCESSION   DBNGZC010000197 DBNGZC010000000
VERSION     DBNGZC010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4499)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4499)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4499
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_700055"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="AC16G0_03645"
     CDS             <1..272
                     /locus_tag="AC16G0_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR5098748.1"
                     /translation="EIVLTQSSGVIVYADGHIHQAPFAPRSLAGRTGRGDTCFATYVG
                     KRLASSPAEACRWAGAVTTLKQERPGPWRGSPSDVEALLAGRPRP"
     gene            321..1508
                     /locus_tag="AC16G0_03650"
     CDS             321..1508
                     /locus_tag="AC16G0_03650"
                     /inference="COORDINATES: protein motif:HMM:NF019585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0002196 - Ser-tRNA(Ala) deacylase activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HIR5098749.1"
                     /translation="MPPAYEREPYLQSLWTEVQDHGMDRGRPFVVLADTILYPEGGGQ
                     PSDRGRIADAPVEDVRTLEGRIRHYLAGPPPSGRVQVELDWSRRFDHMQQHTGQHLLT
                     AVAQDRFGWHTTAFHLGQFLSDIELDVPRAAPAELETLEEAVAAEIRAARPVSARRVS
                     PEVFATLPVRTRGLPEGHEGDIRLVEIAGVDLNTCGGTHVTSTAEIEALKLLGTEPMR
                     GGTRLFYVAGGRLRHLLATHHERTARLRQLVGASDEELVTVVGAKLDQIKSGQRSLRL
                     LEEELAVEAARALAAGTEKVASAHWDQRDLPFLQHVAREFIRLAPNRLALFTAGAGEE
                     GCFLLCAGEGTRTDLAALGREMAGILGGRGGGAGHAFQGKAGALSRRGDALAKLRTLV
                     QGS"
     gene            1715..3280
                     /locus_tag="AC16G0_03655"
     CDS             1715..3280
                     /locus_tag="AC16G0_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098750.1"
                     /translation="MRTIKEAGVLAILLLLGVLVAGSPALAGQASAATPQGGTLKVAT
                     IGEPPNLDVMTLPADLVTMIGQHIFEQLFAFDEKYDIKPMLAQSYNVSADGQTYTIQL
                     RQGVKFHNGQDMTSADVVASLNRWTRVSSRGKIASNSLESVSAPSASTVVIKLKTPFA
                     PLLSFLAFQNTAAAIMPKAIIDKFGDKVVTEYVGTGPYRFEDWKPDFRIRLTRFTGYS
                     PRNEVASGFAGKKTAHLDAIEFYPVTEAGARIAGVESGDYQYAYSVSKEAYPRLSKNA
                     NLMTLVISPGQWGWLIFNKKQGIAANQGVRQAALAALNMEPILRAAYGLTDFYQMEPS
                     YYPKGTAWYSAAGAKNYNQKNPALAKQLLAKAGYKNEPLRIICTKEYEYMYNIGVTAA
                     SQLKEAGFNIDLQVYDWATVLDRRGKPDMYDAFVTGHGFVPDPSLITIMSSQYPGWWD
                     SPEKNDLLAKFNSTTQMTARKQLWDKLQELIYTQVPVIRVGQEKLFDVATAKLVNYWP
                     SVWPSFWNVGLSK"
     gene            3365..4336
                     /locus_tag="AC16G0_03660"
     CDS             3365..4336
                     /locus_tag="AC16G0_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098751.1"
                     /translation="MLGYILRRLIQLVIVLVVVGVVAFLLIHLAPGDPASVMLGSNAT
                     QDQVERLRQQLALDQPLPVQFVAWSSRVIRGDLGISHFAQKPVLQVLAQRAEPTLILT
                     TMSIFVAMLIGVPTGIIAAIRRGTAVDQAALALALVGASVPSFWLGLSLILYFAVRLG
                     WFPSSGILFWQTGGWESLRYYVLPALALGFPNSALITRITRSSMLDVLQQDYVRTAAS
                     KGLPSARVIVRHALRNALVPIITVVGLTVASLMGGAVVTETVFSIPGVGRLVVQSVLR
                     RDYPVIQAAILLVATIYVVINLVVDLIYVWVDPRIRYVEEKSKAKPQ"
BASE COUNT          772 a         1549 c         1417 g          761 t
ORIGIN      
        1 gcgagatcgt cctcacgcaa tcctcgggcg tgatcgtcta tgccgatgga cacatccacc
       61 aggccccgtt cgctccccgc tccctggcgg gtcgcaccgg ccggggcgac acctgcttcg
      121 ccacctacgt cggcaagcga ctggcctcct cccccgcaga ggcgtgccgg tgggcggggg
      181 cggtcacgac actcaaacag gaacggccgg gcccctggcg aggcagcccg agcgacgtgg
      241 aagcgctctt ggctgggcga cctcgaccct gagtgcgtag gacgcaaagc cttgcggata
      301 ccccaaggga gcgaatgacc atgcctcctg catacgagcg agagccgtac ctccagtctc
      361 tttggaccga ggtccaggac cacggcatgg atcgcggccg ccccttcgtc gtcttggcgg
      421 atacgatcct gtacccggaa gggggcggcc agccctccga ccgtggccgg atcgcagatg
      481 ctcccgtcga agacgtccgc acgctggaag gccggatcag gcactacctg gcgggaccgc
      541 cacccagcgg ccgcgtgcag gtcgagcttg actggagccg acgcttcgac cacatgcagc
      601 agcataccgg gcagcacctc ctcaccgccg tggcccagga ccggtttggc tggcacacca
      661 ctgcattcca tttgggccag ttcctctccg acatcgagct ggacgtccca agagccgccc
      721 ccgcggaact cgagacactc gaggaggcgg tggccgccga gattcgcgcc gcccgccccg
      781 tgagcgcccg gcgtgtcagc cccgaggtct tcgcgaccct tccggtgcgc acccgcggtc
      841 tccccgaagg ccacgagggc gacatccggc tggtggagat cgccggggtc gacctcaaca
      901 cctgcggcgg cacccacgta accagcacgg cggagatcga ggcgctgaag ctcctgggca
      961 ccgaacccat gcgtggcggc acgcgcctgt tctacgtggc cggaggccgg ctgcgccatc
     1021 tgctggccac ccaccacgag cgcaccgctc gtctgcgcca gctcgtcggc gcatccgatg
     1081 aggagctggt gacggtggtg ggggcgaagc tcgaccagat caagtcgggg cagcgcagcc
     1141 tccgcctcct ggaggaggag ctggcggtgg aggccgcccg cgccctggcc gccgggaccg
     1201 agaaggtcgc gtcggcccac tgggatcagc gcgatctccc gttcctgcag cacgtagccc
     1261 gcgagttcat ccgtctggcg cccaatcggc tggccctttt cacggcaggc gcgggagaag
     1321 aaggatgctt cctcctctgc gccggcgaag ggaccaggac cgacctggcc gctctcgggc
     1381 gagagatggc cgggatcctt gggggtcgtg gcggcggtgc cggccacgcc ttccagggta
     1441 aggcgggggc cctctcacgt cgaggcgacg ccctggccaa gctccgcacc ctcgtacagg
     1501 gcagttgacg gaaccatcgg cgagcccccg tcccatgccc ggctcatgca ttcacccctt
     1561 cggctttccc cttgacaggc tgtgtggcga ggggagatgc tctgacgcgg cttggctgat
     1621 ctggtgtcag gcgcccgatc tcgtgcgcct gtggtgaatc ttgtcaaaca tgatattgac
     1681 cggaggcggc atcaggagag gaggaggtcg aacgatgcgt accatcaaag aagcaggggt
     1741 cctggctatc cttttgctgc tgggcgtgct tgtggccggg tctccggcgc tggccggtca
     1801 ggcctcggca gcaacccccc agggcgggac gctgaaggtg gcaacgatcg gcgagccgcc
     1861 gaacctggat gtcatgaccc tgcccgccga cctggtgacc atgatcggcc agcatatctt
     1921 tgagcaactg ttcgccttcg acgagaagta cgatatcaag cccatgctgg cccagtccta
     1981 caacgtcagc gccgacggcc agacctacac catccagctc cggcaaggcg tgaagttcca
     2041 caacggccag gacatgacgt ctgcggacgt ggtcgcctct ctcaatcggt ggacccgggt
     2101 gtccagccgc ggcaagatcg cctccaactc gctggagagc gtttctgctc ccagtgcatc
     2161 cacggtggtc atcaaactga agaccccctt cgccccgctg ctgtccttcc tggctttcca
     2221 gaacacggcg gcggccatca tgccgaaggc gatcatcgac aagttcgggg acaaggtggt
     2281 gacggagtat gttggcacgg gtccctaccg attcgaggac tggaagcccg acttccggat
     2341 ccggctcacg cgtttcacgg gctactcccc tcgcaacgaa gtcgccagcg ggttcgccgg
     2401 gaagaagacc gcccatctgg atgccatcga attctacccc gtcaccgagg cgggggcgcg
     2461 catcgcgggc gtggagagcg gtgactacca gtacgcctac tcggtgagca aggaggccta
     2521 tccacgactt tccaagaacg ccaacctgat gaccttggtc atcagtccgg gtcagtgggg
     2581 ctggttgatc ttcaacaaga agcaggggat cgccgcgaac cagggggtgc gccaggccgc
     2641 cttggccgcc ctgaatatgg agccgatcct ccgcgcggcc tatggtctca ccgattttta
     2701 ccagatggag ccgagctatt accccaaggg caccgcgtgg tactccgcgg ccggcgccaa
     2761 gaattacaac cagaagaacc cggccctcgc caagcaactg ctggccaagg ccgggtacaa
     2821 gaacgagccg ctccggatca tctgcaccaa agagtacgag tacatgtaca acatcggcgt
     2881 gaccgcggcg tcccagttga aggaggcggg cttcaacatc gatctccagg tctacgactg
     2941 ggcgacggtc ctcgaccggc ggggcaagcc agacatgtac gatgccttcg tcaccgggca
     3001 cggcttcgtc ccggatccat ccctcatcac catcatgagc tcgcaatacc ccggctggtg
     3061 ggacagcccg gagaagaatg acctcctggc caagttcaac agcaccactc agatgaccgc
     3121 ccgcaagcag ctctgggaca agctgcagga gctgatctac acccaggtcc cggtcatccg
     3181 ggttggccag gagaagctgt tcgatgtggc caccgccaag ctcgtgaact actggccatc
     3241 cgtctggccg agcttctgga acgtcggact ctccaagtaa cgcaaagcca atgacgcgcg
     3301 aagccttcgg gcggtcaggg gccgacaggg ccccagccgt tcgaaggctt cgttgtcgaa
     3361 taccatgctc gggtacatcc tgcgacggct gatccagctt gtcatcgtcc tcgtggtggt
     3421 tggcgtcgtc gccttcctgc tcatccacct cgcgccgggc gatcccgcca gcgtgatgct
     3481 gggttcgaac gccacccagg accaggtcga gcggctccgc cagcagttgg ccctggatca
     3541 acccctcccg gtccaattcg tggcgtggtc ctccagagtc atccgcggcg acctcggcat
     3601 ctctcacttt gcccagaagc cggtcctgca ggtcctggcc cagcgggccg agccgaccct
     3661 catcctgacg acgatgtcca tcttcgtcgc catgttgatc ggcgtaccga ccgggatcat
     3721 cgccgcgatc cggcggggta cggccgtgga ccaggccgcc ctggccctgg ccctggtggg
     3781 cgcctcggtt ccttccttct ggctgggact cagcctgatc ctgtacttcg ccgtccggct
     3841 gggctggttc ccgtcgtcgg ggatcctgtt ttggcagacc ggcgggtggg agagtctccg
     3901 ctactacgtc ctgcctgccc tggccctggg attcccgaac tcggccctca tcacgcgaat
     3961 cacccggtcc agcatgctgg acgtgctgca gcaggactac gtccggacgg cggccagcaa
     4021 agggctcccc tccgcccggg tgatcgtccg ccacgcgctc cggaacgccc tggtgcccat
     4081 tatcaccgtg gtgggtctga cggtcgcctc gctcatgggc ggcgccgtgg tcaccgaaac
     4141 ggtgttcagc attcccggcg tcggccgtct ggtggtccag tcggtcctcc ggcgcgatta
     4201 ccccgtcatc caggcggcca tcctgctcgt ggccacgatc tacgtggtga taaacctcgt
     4261 ggtggatctg atctacgtct gggtggaccc acgcatccga tacgtggaag agaaatcaaa
     4321 ggccaagccc cagtgatcat ggcacgacct gaatccccgc aagccacccc cgcgcagagc
     4381 cccgaggaat cggaggtcga gcggcgtccg tccaggctct cccgtcgcct gcggcgaggc
     4441 cggctgatca tcctcgggac cctcctgacc gtcacgccgc tcatgaccga cctcctggc
//
LOCUS       DBNGZC010000198         2148 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2265982, whole genome shotgun sequence.
ACCESSION   DBNGZC010000198 DBNGZC010000000
VERSION     DBNGZC010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2265982"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="AC16G0_03665"
     CDS             complement(<1..758)
                     /locus_tag="AC16G0_03665"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5098752.1"
                     /translation="MNLRRLSVTGALYLGAATLFVFCGIPLAWMLFTSLKPSGDLFAY
                     PPRLLGNYTLGNFRALITETYFVVYLKNSVLVAGVTVILDIVVATLGAYSLTRYRYPG
                     KEFLANMTLFTYMFAPIMIIIPVYILLKEVNLANSHLGIVLAYTSISLPFTLWLLRAF
                     FQSFPIELEQAAYIDGANRFQSLVYVVIPQALPGIIATSVFAFVVVWNDYLFARILLT
                     DPLLQTMPVGLQNIYESTVVDWGLLMSGAVIVTIP"
     gene            complement(759..1640)
                     /locus_tag="AC16G0_03670"
     CDS             complement(759..1640)
                     /locus_tag="AC16G0_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5098753.1"
                     /translation="MPAHARLALQGILLAVPTFVVIFGFIFFPVGYSVWLSFTNKHAI
                     SPGYDYVGLANYLGLSQEPEFWWSLWRGTIFSVGSIILQILLGLGAALLLNEPFTGRG
                     FIRGITLFPYMLPTIVTILLWKWLLSATFGPVNYILLALGIISSPVIWLGADWLMAST
                     IFVAVWQFFPFVVISLLARLQTIDLQLYEAAAIDGANAWRRFRHITLPELRHVLYVIV
                     LLRTFFMFTKFDVPWLMATGGGLQRLIENLPVYAFRKTFGTFQAGEGAAISVSLFLLL
                     VLLTFLYFWVFRREGEA"
     gene            complement(1954..>2148)
                     /locus_tag="AC16G0_03675"
     CDS             complement(1954..>2148)
                     /locus_tag="AC16G0_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098754.1"
                     /translation="AKNIQIPFLAEVFDSGIIADEIVGMVQGRRSPKDAGQRISERVN
                     DLIKKLGYPVPDPIRGKKKV"
BASE COUNT          469 a          658 c          698 g          323 t
ORIGIN      
        1 ggaatggtca cgatgaccgc gcccgacatc agaagccccc aatcgacgac ggtcgactcg
       61 tagatattct ggagacccac cggcatggtc tgcaggagcg gatccgtcag caggattcgc
      121 gcgaacaggt agtcgttcca gacgaccaca aaggcgaaga ccgaggtggc gatgatcccg
      181 ggcagcgcct gcgggatgac cacgtacacc aacgactgga accggttcgc cccatcgatg
      241 tatgccgcct gctccagctc gatgggaaac gactggaaga aggcccggag aagccacagc
      301 gtgaagggca gggagatgga ggtgtacgcc aacacgatgc cgaggtggga gttggccagg
      361 ttgacctctt tgagcaggat gtacacgggg atgatgatca tgatgggggc gaacatgtag
      421 gtgaagagcg tcatgttggc caggaactcc ttgcccgggt accggtagcg ggtgagactg
      481 taggcgccca gcgtggccac cacgatgtcc aggatcacgg tgacccccgc caccagcacg
      541 ctgtttttca ggtacaccac gaagtacgtc tccgtgatga gggcgcggaa gttgcccaga
      601 gtgtagttcc ccaggagccg tgggggatac gcgaacaggt cgcccgacgg cttcagcgag
      661 gtgaacagca tccaggccag cgggatcccg cagaagacga agagggtcgc cgcgcccagg
      721 tacagggcgc cggtgacaga cagccgccgc aggttcatct acgcctcccc ctcgcgccgg
      781 aaaacccaga agtacaggaa ggtcagcaga acgaggagca agaacaggct gacggagata
      841 gccgcgccct ccccggcctg gaacgtgccg aaggtcttcc ggaaggcgta cacgggcagg
      901 ttctcgatca agcgctgcag gccgcccccc gtggccatca gccacgggac gtcgaacttc
      961 gtgaacatga agaaggtccg caagaggaca atgacgtaga ggacgtgacg cagctcgggg
     1021 agcgtgatgt gccggaagcg ccgccaggcg ttcgcgccgt cgatggcggc ggcctcgtag
     1081 agctgcaaat cgatggtctg caggcgcgcc aagaggctga tcaccacgaa ggggaaaaac
     1141 tgccacacgg ccacgaagat ggtggaggcc atgagccagt cggcccccag ccagatcacc
     1201 ggcgagctga tgatccccag cgccagcagg atgtagttca ccggtccgaa ggtcgcgctc
     1261 aggagccact tccacagcag gatggtcacg atggtcggca gcatgtaggg gaacagcgtg
     1321 atcccgcgga tgaacccccg cccggtgaac ggctcgttca gcagcagggc cgcccccaag
     1381 ccgaggagga tctggaggat gatgctgccc acggagaaga tggttccccg ccagagagac
     1441 caccagaact ccggctcctg gctcagcccc aggtaattgg ccagaccgac gtagtcgtat
     1501 ccgggagaga tggcgtgctt gttggtaaaa ctcagccaga cggaatagcc gacgggaaag
     1561 aaaatgaagc cgaagatgac gacgaaagtc ggcacggcca ggaggatgcc ctgcagggcc
     1621 agccgggcgt gcgcgggcat cgccctcttc ccactactcg ccggccctgc gagctcctcc
     1681 attagatgca cccccttcct tctcccaaaa cttttcactc acggcgcgag aatgccaccc
     1741 ggctcaagca aatccccccc accccctttt accaaagggg gcgaggggga atttgacgcg
     1801 actgaggacc acagtggatg gcgatgcccc ttacccgaga gccgcgtcgt ccgcgcgcat
     1861 gcctgaggaa gctactgtca tacccgagtg ccgtcacccc ggaggagacc ggggtcgaga
     1921 agccctggca agcgcgcacc acgtgggccg ccttcagacc ttcttcttgc ctcggatggg
     1981 atccggcacg gggtagccta gcttcttgat gaggtcgttc acccgctcgc tgatccgttg
     2041 gccggcgtcc ttcggcgatc ggcgcccctg gaccatcccg acgatctcgt cggcgatgat
     2101 cccagagtcg aacacctcgg ccaggaaggg gatctggatg ttcttggc
//
LOCUS       DBNGZC010000199         1407 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1288500, whole genome shotgun sequence.
ACCESSION   DBNGZC010000199 DBNGZC010000000
VERSION     DBNGZC010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1407)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1288500"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="AC16G0_03680"
     CDS             complement(<1..438)
                     /locus_tag="AC16G0_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098755.1"
                     /translation="MEVTAEHPELLDPGGECPRDTVGDGGVQEVVTEFLKDLLDGEPH
                     AHGELWIGRGGDDGPVVLAKKISIGRDLLRLLSKIIRHPEFVVEIDFTPEIHRPYFIP
                     GGSTVVGADLLPVGEAAHPQAQQGHTLRSGLQALGWIHGSTIHT"
     gene            638..1018
                     /locus_tag="AC16G0_03685"
     CDS             638..1018
                     /locus_tag="AC16G0_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098756.1"
                     /translation="MPLDLAKVHELEKIPGTEQFRLVKVHPTASLGRQGEPPIWIQDG
                     KLYYEGGEEVRNPPEWFWEDLAKIDPERRKVLGVRLPGKARHLEERTEAAVQPMKRKR
                     PRSFIRRKLTVRPADPAPPDAGKE"
     gene            1022..>1407
                     /locus_tag="AC16G0_03690"
     CDS             1022..>1407
                     /locus_tag="AC16G0_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098757.1"
                     /translation="MAAITVSGYSQPIARGVLYTKWVFATGLTCGTGSYGKPLKAPHY
                     PDKTISVRGPFVSGVTVVILGSNTATNTTATTKMFMLHSASGADLSFATGKTRTMLEN
                     PLWIMPYLTARTGTKGAATVEIIAQST"
BASE COUNT          305 a          437 c          423 g          242 t
ORIGIN      
        1 cgtatggatg gtagagccat gaatccagcc gagtgcctga agacccgacc gaagagtgtg
       61 gccctgctgg gcctggggat gagccgcctc gcctacgggg agcaaatctg ctccgactac
      121 tgtgctaccc cctgggatga aatatggacg gtgaatttcg ggggtaaaat ctatctccac
      181 gacaaactct gggtggcgga tgatcttcga aagcaggcga aggagatccc ggcctatgga
      241 gattttcttc gccagcacga ccggcccatc atcaccacca cgccctatcc agagttcccc
      301 atgagcgtgc ggttccccat cgaggaggtc cttcagaaac tcggtgacga cttcctgaac
      361 tccaccgtcg cctactgtat cgcgtgggca ctcaccaccg gggtcaagga gttctggatg
      421 ttcggctgtg acttccacta cccgaatcag acacgggccg aggagggagg ccaatgtgcg
      481 gcctatctcc tggggctggg acgccatttc ggcatgacct tcaagatccc gcccagcacg
      541 accctcctcg gcgcctacca tgcgaagcct gttggcgatt cgatccgacg accgttgtac
      601 ggctacgcga aacaaccctt tatcccggag gacggcgatg cctctcgacc tggcgaaagt
      661 acacgagctg gagaaaatac cggggaccga gcaattccgg ctggtcaagg tccacccgac
      721 agcctccctg gggcgccagg gcgagccgcc gatctggatt caagacggca aactctatta
      781 cgagggtggg gaggaagtca ggaacccgcc ggagtggttc tgggaggacc tggccaagat
      841 cgacccggag cgccggaagg tcctgggcgt ccgcctgcct gggaaggcgc gacacctgga
      901 ggagcggacg gaggcggcag tccagccgat gaaacgaaag cgcccgcgtt cgtttatccg
      961 gcggaagcta actgtccgac ccgccgaccc ggccccgcct gacgcgggga aggagtaacc
     1021 catggctgca atcacggtga gcggctacag ccagccgatt gcccgcgggg tgctgtacac
     1081 aaaatgggtc ttcgccacag gcctcacctg cgggactggc agttacggca agccactaaa
     1141 ggcacctcac tacccggaca agaccatcag cgttcgcggt ccctttgtgt caggtgtcac
     1201 ggttgtcatc cttggatcga acaccgcgac caacacaacg gcaacaacca agatgttcat
     1261 gctgcattcc gcgtctggag ccgatctatc atttgccact gggaaaaccc gcaccatgct
     1321 ggaaaacccc ctctggatca tgccgtacct cacggccagg actgggacca agggggcggc
     1381 cacggtggag atcatcgcac agagtac
//
LOCUS       DBNGZC010000200         2078 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_17045, whole genome shotgun sequence.
ACCESSION   DBNGZC010000200 DBNGZC010000000
VERSION     DBNGZC010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_17045"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..308)
                     /locus_tag="AC16G0_03695"
     CDS             complement(69..308)
                     /locus_tag="AC16G0_03695"
                     /inference="COORDINATES: protein motif:HMM:NF015957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR5098758.1"
                     /translation="MSDNPGARPKLRRVKGRHWVGGVCAGVGYWLGVATWLVRLIWTV
                     SILAYGFGLLLYVLLWIFMPVWDHVPEDYEERAGG"
     gene            complement(439..768)
                     /locus_tag="AC16G0_03700"
     CDS             complement(439..768)
                     /locus_tag="AC16G0_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHCW motif cupin fold protein"
                     /protein_id="HIR5098759.1"
                     /translation="MQIQDVPFCVTDWSTVPRTEHPGESGVAFWRTLEVGNIRVRMVE
                     YSPGYRADHWCSRGHVLLVLEGELVTEIRNGKAHTLRPGESYQVAENAEPHRSHTQTG
                     AKLFIVD"
     gene            1070..>2078
                     /locus_tag="AC16G0_03705"
     CDS             1070..>2078
                     /locus_tag="AC16G0_03705"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HIR5098760.1"
                     /translation="MRDVPEDLREILRSVSRSFYLTLAVLPADLRGPVGLAYLLARAA
                     DTIADTRLIPRVDRLHHLDILRGTLDASVSPGSPDVAQVLSHSQQIPAERELLAHLPE
                     CVVLYWGLPDGDRRRIRNLLFTLTEGMQTELRTFPGEDEGRIVALASRADLDRHTYYA
                     AGCVGEFWTEMAVAHRPACRSWNADSMKRRGVRFGQGLQLTNVLRDLAHDLRIGRCFL
                     PREDLRTLGLTPNDLLDPGAIARLRPLLRELLSRTLEHLADGWSYTLTIPRGEMRMRL
                     ACIWPLFIGLRTLHRIWEAPNLLDPGVRVRVSRPAVYAILARSSALAWSDYALDRYYR
                     TL"
BASE COUNT          358 a          723 c          629 g          368 t
ORIGIN      
        1 gggacctact cacagcctag ggggacaccc cttccctgtc aagatgcccc cctgcgaccc
       61 tcccccgact acccgccggc ccgttcctca taatcttcgg ggacatgatc ccaaaccggc
      121 atgaaaatcc agagaaggac gtacagcaag agcccaaagc catacgccag gatcgagacc
      181 gtccagatca accgcacaag ccaggtcgcg acaccgagcc agtacccgac gccggcgcac
      241 acgccgccca cccagtgcct ccccttgacg cgtcggagct tgggtcgggc tccagggtta
      301 tcactcatcg caactccttt cgccattggt ggaagtgccc acagcttccg gatgggttcc
      361 cggcgcgggg cacccgccgg aggtgggtac ccatccaatg gatgggtacc cggcagatgg
      421 actttctgca gcagcccctt agtcgacgat gaacagcttg gcacccgtct gcgtgtgcga
      481 acgatgcggt tcggcgttct ccgccacctg gtaactctcg cccggcctca aggtgtgcgc
      541 cttcccgttg cgtatctcgg tcaccagttc gccttccagg acaagaagca catgacctcg
      601 gctgcaccaa tgatccgccc ggtatccagg cgagtattcg accatccgca cgcggatatt
      661 gccgacctca agagttcgcc aaaaggcaac tcccgattcg ccggggtgtt ctgtgcgcgg
      721 aacggtactc cagtccgtga cgcagaaggg aacatcctgg atctgcattg ccaatcccct
      781 gtctcccgga cggcactcgc gtccgagcag tgatcccccg ccgggctcac ccggaatgcc
      841 cggccatggg ggcgcccggc tgaaggctat cggaggctca ggggggtgtc aacacgaaag
      901 ccgctgcggg ccacgcgacc gggcggttcg tgagcgcgga gacgtactcc gattgtcacc
      961 gggcgaaacc cctgacgagg ccgggggctc ctaatcccgc ttcaggagat acggcggatg
     1021 tggtatggtt cgaggggacg caaggggacg tatcctggcg aggtcgacgg tgcgtgatgt
     1081 tccggaggat ttgcgagaga tcctgaggtc ggtgagccgg tcgttctacc tgaccctcgc
     1141 cgtgctcccc gcggatttgc gggggccggt gggcctggca tacctcttgg cacgagccgc
     1201 ggataccatc gccgacacac gactcatccc tcgggtcgat cgcctccatc acctggacat
     1261 cctccggggg acgctcgacg cctccgtctc cccgggatct ccggacgtcg cgcaggtcct
     1321 cagccattcg cagcagatcc ccgccgagcg ggaacttctc gcgcatcttc cggagtgtgt
     1381 cgttctgtat tgggggctgc cggacggcga tcgccggcgg atccgcaacc tgctgttcac
     1441 gctcactgag ggcatgcaga ccgagcttcg cacctttccc ggagaagacg agggccggat
     1501 cgtggccctg gcatctcgtg cggacctgga ccggcatacc tattacgcgg ccgggtgcgt
     1561 gggagaattc tggacggaaa tggctgtggc ccaccgacct gcgtgtcgct cctggaatgc
     1621 cgacagcatg aaacggcggg gcgtccgctt cggccaaggc ctccagttga cgaacgttct
     1681 ccgggatctg gcgcacgatc tcagaatcgg ccgatgcttc ctcccgcggg aggatctcag
     1741 gacgctgggc ctcactccca acgacctcct cgatcccggc gccatcgcgc gactccgtcc
     1801 gctgctgcgc gagctgctca gccgcaccct cgaacacctt gccgacgggt ggagctacac
     1861 cctgacgatc ccacgagggg agatgcgcat gcgactcgcc tgcatttggc cgctgttcat
     1921 cggacttcgc acgctgcacc gcatctggga ggcacccaac ttgctcgacc ccggcgtgcg
     1981 cgtacgagtc tcccgacccg cagtgtatgc gatcctcgcc cggtcctccg ccctggcctg
     2041 gtccgactac gccctcgacc ggtactaccg gactctcc
//
LOCUS       DBNGZC010000201         2238 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1484055, whole genome shotgun sequence.
ACCESSION   DBNGZC010000201 DBNGZC010000000
VERSION     DBNGZC010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1484055"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..330)
                     /locus_tag="AC16G0_03710"
     CDS             complement(55..330)
                     /locus_tag="AC16G0_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098761.1"
                     /translation="MIITPEQAALNAQERFDHLLDLVRQASHDGQRIDTVERDLMRHL
                     LALGHDPLALFIAKQGDGDLGPTMQTSEGRTLHRLPEAHAGIVARRM"
     gene            491..1921
                     /gene="tnpC"
                     /locus_tag="AC16G0_03715"
     CDS             491..1921
                     /gene="tnpC"
                     /locus_tag="AC16G0_03715"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HIR5098762.1"
                     /translation="MRAKGMVRCSNCQRLQARVDALEAELAALKQVVAQLGQQRAAAR
                     KDSSTSSKPPSSDIVKPPKPPPPEGQDKRRIGGQPGHPKHERVAFPPEAINSGSIDHH
                     LDSCPSCGHDLQPALTIAPRVVQQVDIREVPLSLEEHRSHPGWCPHCQKMYEAPLPPR
                     IARGGLIGPSLTTLIAYLKGACHASFSTIRKFLRDVVRVTVSRGELARIIAKVSRALE
                     RPYEELLEGLPTEARLNVDETGHKQNGVRMWTWCFRAGLYTLFKLDPTRSADVLTAVL
                     GTEFNGVLGCDYFSAYRRYQREFGVLLQFCLAHLIRDVKFLTTLPDARDRAYGEGLRE
                     ALRELFAVIHRREGLSAAAFRSQLEAARAEVLRRGTRQVPETRHSRNLALRVETHGES
                     YFRFLTEPGVEPTNNLAEQAIRFVVLDRLVTQGTRSEAGDRWCERIWTAIATCAQQGR
                     SVYSYLAAVVEAWFAGTAAPSLLPEG"
BASE COUNT          392 a          717 c          759 g          370 t
ORIGIN      
        1 gccgatcacc cgccgtgcag tgtgaattac acagccggac ggacaccccg tcctctacat
       61 tcttcgtgca acaatgccgg catgggcctc gggcagtcga tgaagcgtcc ggccctcgga
      121 tgtctgcata gtcggcccga ggtcgccatc gccctgcttg gcgataaaga gggccagcgg
      181 gtcgtgccca agggcgagca gatggcgcat caggtcccgc tccacggtgt cgatccgctg
      241 gccgtcatga ctggcctgcc gcacgaggtc cagcaggtga tcgaacctct cctgggcgtt
      301 gagggcggcc tgctccgggg tgatgatcat gacgaggcct cctgtgtgca gttccatcgg
      361 ggtgtagcaa ccgatattct gcctcacgga ggcctcgttt tccactgcct gtcgccacaa
      421 ctcgatatca aagaacaaaa tccagaggga gcgggaggcg agtcacgccc aatttgtaga
      481 atagaccgcc atgagagcca aggggatggt ccgctgctcc aattgccaac ggctccaggc
      541 gcgagtcgat gccctggagg cggaactcgc cgccctcaag caggtcgtcg cccaactggg
      601 ccagcaacgc gccgcggcgc gtaaggactc ctcgacctcg tccaaacccc cgtcctccga
      661 catcgtcaag ccgcccaagc cgcccccgcc ggagggccag gacaaacgcc ggatcggcgg
      721 ccagcccggc catcccaagc acgaacgcgt cgccttcccg cccgaggcca tcaacagcgg
      781 gtccatcgac catcacctcg actcctgccc ctcgtgcggc catgacctgc aacccgccct
      841 gaccatcgca cctcgggtgg tccagcaggt cgacatccgc gaggtcccgc tgtccctcga
      901 ggagcatcgc agtcatccgg gatggtgccc gcactgccag aagatgtacg aggccccgct
      961 cccgccgagg atcgcgcggg gtggtctgat cgggccgtcc ctgaccacgc tgatcgccta
     1021 cctcaagggg gcctgtcatg cctccttctc gaccatccgc aagttcctcc gcgatgtggt
     1081 gcgggtgacg gtctcgcgtg gggaactggc ccggatcatc gccaaggtca gccgggcgct
     1141 ggagcggccc tatgaggaac tgttggaggg cctgccgacc gaggcgcgtc tgaacgtcga
     1201 tgagaccggc cacaagcaga acggcgtgcg gatgtggacc tggtgctttc gcgccggcct
     1261 gtacaccttg ttcaagctcg acccgacccg cagcgcagac gtgttgaccg cggttctggg
     1321 caccgagttc aacggggtgc tgggctgcga ctacttctcg gcgtaccgcc gctatcagcg
     1381 cgagttcggc gtgctgctcc agttctgcct ggcgcacctg atccgcgatg tgaagttcct
     1441 gacgaccctg cccgatgcgc gggaccgtgc ctacggggag ggcctgcgcg aggcgttgcg
     1501 ggagttgttc gcggtaatcc accggcggga ggggttgtcc gccgcggcat tccggagcca
     1561 actggaggcg gcgcgggccg aggtgttgcg tcgtggtacc cggcaagtgc cggagacgcg
     1621 gcacagccgc aacctggcgt tgcgggtgga gacgcacggg gagagctact tccggttcct
     1681 gacggagccg ggagtggagc cgacgaacaa cctggcggag caggcgatcc gattcgtggt
     1741 gctcgaccgg ttggtcacgc aggggacgcg gagcgaggcg ggcgaccgct ggtgcgagcg
     1801 gatctggacg gcgatcgcga cctgcgctca gcaagggcgt tcggtgtact cgtacttggc
     1861 ggcggtggtc gaggcgtggt tcgccgggac agcggcacca tcgctattac ccgagggctg
     1921 aggagcgcgt cgcccgagag agccggtgag gtgctcaggt gcatggtcct ccgggaccgg
     1981 gacgtcctga agatcgcacg ccggtgagga caacgacgag agattcagga gtccatcctg
     2041 cgcggggtgg aggacgggca gggttcgtgg tgaggagagc caatgattac ccattggcga
     2101 gtggacccgt acgcgcacac ctcatgtggt aacagtatca cagcactggt gaacggcaac
     2161 gattccggtg agaaaccgcc ctcgccggaa tgcattcggg ctcatctccc aaccgagtgg
     2221 gtagaaaatc gcggttga
//
LOCUS       DBNGZC010000202         1734 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1386527, whole genome shotgun sequence.
ACCESSION   DBNGZC010000202 DBNGZC010000000
VERSION     DBNGZC010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1386527"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="AC16G0_03720"
     CDS             complement(<1..817)
                     /locus_tag="AC16G0_03720"
                     /inference="COORDINATES: protein motif:HMM:NF015038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HIR5098763.1"
                     /translation="MPVEHPPTSWSDASPVSLLARPEETSTCSAPPIPQGPCPVCPRL
                     AQEFEPWRQAAYWQAMHQRSVQREKLLHDENEQLHARIRFLQQQLFGRKTDSTTTAEG
                     SPRPDRTPRPRGQRRGKPGPKRRDYSHLPAVEQILELPAEQQCCGRCGQPFASFPGTD
                     DTTVVEVEVRAHRRIYRRRRYRPTCSCGHHAGIITAPPPPRVIPKSLLGVSVWGTILL
                     DKFLFYRPTYRLLEDLRTEGLDLSQGTLTDGLQRLLPLFEPVYDLLVEHSQQQA"
     gene            1068..>1734
                     /locus_tag="AC16G0_03725"
     CDS             1068..>1734
                     /locus_tag="AC16G0_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098764.1"
                     /translation="MSAHHVNPPTAVCPPDLEPLQQQIHQRLDEIIAYCLHESGPTSF
                     RGFEKALLSLLQSLGCLLIPLFLKARHARLDLTTWTRARGYRVSDKHDKRTLTTSCGE
                     VTYERAYLVPRRGGGPGVHPLDVALGLTRDAYSPLIIGWFCRLATRASFQIASGLGEM
                     FLGSAPPPSAIEEWVLGLGRPAYVYLSDGPLPEGDGDVLVIEIDGKAVPTATDHELAR
                     RRKP"
BASE COUNT          289 a          528 c          617 g          300 t
ORIGIN      
        1 gggcctgctg ctgactgtgc tcgaccaaca ggtcgtacac cggctcgaac aggggcagga
       61 gccgttgcag gccgtcggtg agcgtcccct gcgacaggtc caggccctcg gtccgcaggt
      121 cctccagtaa ccggtacgtg gggcggtaga agaggaactt gtcgagcagg atcgtgcccc
      181 agacggagac cccgaggagg ctcttgggga tcactctggg cgggggtggc gcggtgatga
      241 tgccggcgtg atggccacag gagcaggtgg gccgataccg tcgccgccgg tagattcgcc
      301 gatgggcgcg gacttcgact tccacgacgg tcgtgtcgtc ggtgcctggg aacgaggcga
      361 agggctgtcc acagcgccca caacactgct gctcggccgg tagttcaagg atctgctcga
      421 cggcgggcag gtgggaataa tcccgacgct tggggccggg cttgccacgt cgttgcccgc
      481 gtggccgtgg tgttcgatcg gggcgggggg agccctcggc ggtggtggtc gagtcggtct
      541 tgcgcccgaa gagctgctgt tggaggaaac ggatgcgggc gtggagttgc tcgttctcgt
      601 cgtggaggag tttctcgcgc tggacggacc gctgatgcat ggcctgccag taggcggcct
      661 ggcgccaggg ctcgaactcc tgggccagac gtgggcagac cgggcacggt ccctggggga
      721 tggggggagc cgaacaggtc gaggtctcct cgggccgggc caggagagag accggagaag
      781 cgtcagacca ggaggtcggg ggatgctcca caggcatgtc gcccgttgta gtcgcggacg
      841 gacgggaaag ccagagagcc ggacggggtt accgaaactt taccacctcg tgatcgtaag
      901 cccttgaatc atcccgagaa agcgtgtgtt tccgagtgtt gacctcgtcc gaaggcactc
      961 gcgatgggct attattcgcg acgacgcggt gaaaggtgag gctcagggga cgtatcctga
     1021 ccgctgatca acatgtcaca aacgggaatg atcaggagtc cgcccccatg agcgctcatc
     1081 acgttaaccc accaaccgcg gtgtgtccac cggacctcga accgttgcaa cagcagatcc
     1141 atcagcggct cgacgagatc atcgcctact gcctgcacga gtcgggcccc acgtcgttcc
     1201 gcggcttcga gaaggccttg ctttcgcttc tgcaatcgct gggctgtctg ttgatcccgc
     1261 tgttcctcaa ggcacgccac gcccggctgg acctgacaac ctggacgcgg gcacgcggct
     1321 atcgcgtctc ggacaagcac gacaagcgga cgctcacgac cagttgcgga gaggtcacct
     1381 atgagcgggc ctacctcgtg ccacgccgcg gtggtggccc cggcgtgcac ccgttggatg
     1441 tcgcgctcgg tttgacgcgc gatgcctaca gcccgctgat catcggctgg ttctgccggc
     1501 tggccacccg ggcgagcttc cagatcgcca gcgggctcgg ggagatgttc ctggggtccg
     1561 cgccgccacc ttcggcaatc gaggagtggg ttctgggtct gggccggccc gcctacgtct
     1621 accttagcga cggccccttg cctgaaggcg acggcgacgt gctggtcatc gagatcgacg
     1681 gcaaggcggt ccctacggcc acggatcacg agctggcgcg gcgccgcaag ccgt
//
LOCUS       DBNGZC010000203         4578 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1972823, whole genome shotgun sequence.
ACCESSION   DBNGZC010000203 DBNGZC010000000
VERSION     DBNGZC010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1972823"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="AC16G0_03730"
     CDS             <1..254
                     /locus_tag="AC16G0_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098765.1"
                     /translation="DAGERVLRRASDGNVAAVVTSVRAIGFSAPLNVTDEIRVPDDET
                     LEAFQAAGDVVTVLTRRRALGFSAIVGKWTIIDRFLLGR"
     gene            complement(313..1224)
                     /locus_tag="AC16G0_03735"
     CDS             complement(313..1224)
                     /locus_tag="AC16G0_03735"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR5098766.1"
                     /translation="MKLVQFFLPGKGKRVGLIRGDRVLDITAPEEGVMCVLDLVEQGK
                     SATGLLKRTEWLARRLHRKAVDWPALQRPPSRRAPHLLIPIDPPEVWGAGITYKRSAE
                     FRDEDSQAVGGWRGMYDFVYESPRPELFFKATASRCVGPNAPLMIRSDSHLTAAEAEL
                     AIILGARGAVVGFTACNDVSAWDLERENPLFLPQSKIYLGCCGLGPCLVTPDEIGDPY
                     ALQIRCSLIRGGKTVFSEAVNTAQFHRKLEDLVSWLIRDNPIPAGTALSTGTGIMLPN
                     ELALRDGDQVDIEIQGIGRLSNPVRCL"
     gene            complement(1268..2533)
                     /locus_tag="AC16G0_03740"
     CDS             complement(1268..2533)
                     /locus_tag="AC16G0_03740"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098767.1"
                     /translation="MKRTTWILALLLLAAAAPARAATEIKFYYPVHLTGPLVKEMEAI
                     VADFHKANPGIRVEPVWGGTYPENMQKTVAALQAGNPPDVAVHLAVDLVTLRDLDAVV
                     SLDEFIQREGGQKFLSDFFPGFLSDMKADGKMWAIPFQRSTPILYINKDLFKKAGLDP
                     NKPPTNWDELLTTAQKATVRDASGQASIWGLTIPDDIWLLMSFIYQNDGRMNSPDFKR
                     VYFGEKASGDALQFWVDLVNKHKVMPRHRSFPESVQDFAAGKTAMLIHSTGNLRFVRD
                     SAKFEWGTAFLPKHKKQAVTIGGAGFYVFKKAPREKQEAAWQFIKFATSPEIQARWNI
                     GSGYIATRKSAFELPLMKEYTAKLPQALVARDQLPFALPEMSAHDNWKVRNVLLTTFQ
                     EVLDGKYPAEEGMRRAQGEADKILAPDQK"
     gene            complement(2560..3390)
                     /locus_tag="AC16G0_03745"
     CDS             complement(2560..3390)
                     /locus_tag="AC16G0_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5098768.1"
                     /translation="MRSRPLWNFTAHGLLLLAALLMVLPFVWIALTALKERNEVFSLV
                     PQWLPAHPTLKNFAGVLDAMPFWRYYWNTLVVTFGILGVQLVTITLAAYAFARMDFRG
                     RDVLFMLFLVQLMIAPQSTVIQNYLTISSLGLLDTRLAIMAPYLASAFGTFLLRQTFK
                     SLPKELEEAAWLDGCGGLRFLIHIAVPLAAPGYLAFALVSLTSHWNEFFWPLVVTETS
                     KARTLTLGLGIFAQTAEGSADWTMLMAATLMVIAVPLALFLLFQRRFINSFMQSGLSG
                     "
     gene            complement(3383..4318)
                     /locus_tag="AC16G0_03750"
     CDS             complement(3383..4318)
                     /locus_tag="AC16G0_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5098769.1"
                     /translation="MGAIPLTGVEAAGCRGPEWVWRRMLHAARPYLYILPAFVFLFIF
                     THYPILKTFYLSLFKWNLATPRQEFVGPANYVQAWSTPLFWQVLRNNVFFALWTIPFS
                     LGVAMALALLINQRIRGLAFYRAALFYPTIIPMAAAAMIWLWILTPSYGLLNYYGKPL
                     GLPDIHWLGDSRFALWALALVGVWKRVGYYMVIFLAGLQIIPEYLYEAAILEGANAWQ
                     RFRRVTFPLLSPTTFFVAVMAVIDSFQAIDQVYLMTQGGPGNETNLFIFYIYQNAFRF
                     FDMGYASAVSGILFLLLLGLTALAFSLLQRRVHYA"
BASE COUNT          887 a         1458 c         1440 g          793 t
ORIGIN      
        1 tggatgccgg cgagcgagtt ctccggcgcg cgtcggacgg caacgtcgcg gccgtcgtga
       61 cctcggtgcg ggcgatcggc ttcagcgcgc ctctcaatgt gaccgatgag atccgggtcc
      121 cggatgacga gaccctggag gcgttccagg cagcgggaga cgtagtcacg gtgctcaccc
      181 gacgccgcgc cctcggcttc agcgcgatcg tgggcaagtg gaccattatc gatcgcttcc
      241 tgctcggacg gtaaccagga aaaaccgaca gcaatatcag gaacgccgtc cctgtcgcca
      301 tccctgcccg ccttagagac atcgcacggg attgctcaac ctcccgatcc cctggatctc
      361 gatgtccacc tgatccccgt cgcggagcgc aagctcgttg ggtagcatga tccccgtgcc
      421 cgtggaaaga gccgtccccg ccgggatcgg attgtccctt atcagccagg agaccagatc
      481 ctccagtttg cgatgaaact gagcggtgtt caccgcttcg gagaagaccg tcttccctcc
      541 ccggatgagg ctgcaccgga tctggagtgc gtagggatcg ccgatttcgt ccggagtcac
      601 caggcacgga cccaggccgc agcacccgag atagatcttc gactgcggca ggaagagggg
      661 attctcccgc tccagatccc aggcggacac gtcgttgcat gccgtgaagc cgaccacggc
      721 cccgcgggcc cccagaatga tcgccagctc cgcctccgcc gccgtcagat gggaatcgct
      781 tcggatcatc agcggcgcgt tcggcccgac gcagcgcgag gccgtggcct tgaagaacaa
      841 ctccgggcgc gggctctcat acacgaagtc gtacatgccc cgccaccccc ccaccgcctg
      901 gctgtcctcg tcccgaaact cggcgctccg cttgtaggtg atcccggccc cccagacctc
      961 gggaggatcg atggggatca ggaggtgcgg cgcccggcgc gaggggggcc gctggagtgc
     1021 cggccaatcc actgccttcc ggtgcagccg cctggccagc cactccgtcc gcttcaacag
     1081 acccgtagcg ctcttccctt gctccaccaa gtcgaggacg cacatcacac cctcttcggg
     1141 agccgtgatg tccagcaccc ggtccccacg gatcagcccg acccgcttcc ccttccccgg
     1201 caggaagaac tgcacgagtt tcatggcggc ccctccccgg aagcgccgct cgctcccacc
     1261 ggctgcccta cttctggtcc ggcgcgagga tcttgtccgc ctcgccctgg gcccgccgca
     1321 tgccctcttc cgccgggtat ttcccgtcca gcacctcctg gaacgtggtc aagagaacat
     1381 tgcgaacctt ccagttgtcg tgggcgctca tctcgggcaa agcaaagggt aactgatccc
     1441 gcgccaccag ggcctggggg agcttcgcgg tgtactcctt catgagcggc agctcgaagg
     1501 ccgacttgcg ggtggcgatg tagccggagc cgatgttcca gcgagcctgg atctcgggcg
     1561 aggtggcgaa cttgatgaac tgccaggccg cctcctgctt ctccctgggg gccttcttga
     1621 agacgtagaa gcccgctccg ccgatggtca ccgcctgctt cttgtgcttt ggcaggaagg
     1681 cggttcccca ctcgaatttc gccgagtccc ggacgaatcg cagattccca gtcgagtgaa
     1741 tcagcatcgc cgtcttgcct gcggcgaagt cctggaccga ctccgggaag ctgcgatggc
     1801 gcggcatgac cttgtgcttg ttcacgaggt ccacccagaa ttgcagggcg tcccccgacg
     1861 ccttctcgcc gaaatacacc ctcttgaagt cggggctgtt catccggcca tcgttctggt
     1921 agatgaacga catgagcagc cagatgtcgt ccgggatcgt cagcccccag atgctggcct
     1981 ggcccgaggc gtcccggacc gtggccttct gggcggtggt gaggagctca tcccagttcg
     2041 tgggtggctt gttcggatcc agccccgcct tcttgaagag gtctttgttg atgtagagga
     2101 tgggcgtgct ccgctggaag gggatggccc acatcttccc gtccgccttc atgtcgctga
     2161 ggaacccagg aaagaaatcg ctcaggaatt tctgaccgcc ttcgcgctgg atgaactcat
     2221 ccagggacac cacagcatcc agatcccgca gcgtcaccaa gtcaacggcc agatgcaccg
     2281 ccacgtcggg gggattgccg gcctggaggg ccgccaccgt cttctgcatg ttctccggat
     2341 aggtgccgcc ccacaccggt tcgacccgga tccccggatt ggctttgtgg aagtcggcca
     2401 caatggcctc catctccttc accagggggc cggtgagatg aaccgggtaa tagaacttga
     2461 tctccgtcgc ggcccgggcc ggggccgcgg ccgccagcag cagtaacgcc aggatccagg
     2521 tcgttcgttt catgggtctc ctcctgcggg ctcgcgcgat catccgctca gacccgactg
     2581 catgaagctg ttgatgaacc ggcgctgaaa gagcaggaat agggccagcg gcaccgcgat
     2641 caccatcagg gttgccgcca tgagcatcgt ccagtccgcg gatccctcgg ccgtctgggc
     2701 aaagatcccc agccccagtg tcagcgtgcg ggccttgctg gtctcggtga cgaccaacgg
     2761 ccagaagaac tcgttccagt gggaggtgag gctcaccagg gcgaaggcca agtatcccgg
     2821 tgctgccagg gggacggcga tgtggatcag gaagcgcagc cccccgcagc cgtccagcca
     2881 cgccgcctct tccaactctt tgggcaggct cttgaaggtc tgccgcaaca ggaaggtccc
     2941 gaaggccgag gccagatagg gggccatgat ggccagccgg gtatccaaga gccccaagct
     3001 gctgatggtc aggtagttct ggatgaccgt gctttgcggc gcgatcatca gttgcaccag
     3061 gaacagcatg aagaggacgt cgcgcccccg gaaatccatc cgggcgaatg cataggcggc
     3121 aagcgtgatg gtcaccagtt gcactccgag gatcccaaag gtcacgacca gcgtgttcca
     3181 gtaatatcgc cagaacggca tggcatccag gacgccggcg aagttcttca gggtcggatg
     3241 ggccggcagc cactggggga ccagcgagaa gacttcgttt cgctccttga gggccgtcaa
     3301 ggcgatccag acaaagggca acaccatcag aagcgccgcc aagaggagca ggccgtgagc
     3361 tgtgaaattc cagagggggc ggctacgcat agtgcacccg tcgctggagg aggctgaagg
     3421 ccaaggccgt cagccccagc aggagcagga aaaggatccc cgagaccgcc gatgcgtaac
     3481 ccatgtcgaa aaagcggaag gcgttctggt agatgtagaa gatgaacagg ttggtctcgt
     3541 tgcctggtcc gccctgcgtc atgagataca cctggtcgat cgcctggaag gaatcgatca
     3601 ccgccatgac ggcgacaaag aaggtagtgg gtgagagtag cgggaaggtg acccgccgga
     3661 atcgctgcca cgcgttggcc ccctccagga tggcggcctc gtacaggtac tccggaatga
     3721 tctggaggcc agccaggaag atgaccatgt agtatcccac ccgcttccac acccccacta
     3781 gcgccagcgc ccagagggcg aagcggctgt ccccgagcca gtggatatcc ggcaggccca
     3841 gcggcttccc gtagtaattc agcaagccgt aactcggggt gaggatccag agccagatca
     3901 tggcggctgc cgccataggt atgatggtcg ggtaaaacag cgccgcccgg tagaacgcca
     3961 ggccgcggat cctctggttg atgaggaggg ccaatgccat ggcgactccc aggctgaagg
     4021 ggatcgtcca taaggcgaag aagacgttgt tccgcaggac ctgccaaaaa agtggagtgg
     4081 accaggcctg cacgtaattg gccggcccca cgaattcctg cctcggcgtg gccagattcc
     4141 atttgaagag gctcagatag aatgtcttca aaatggggta gtgggtgaag atgaaaagga
     4201 agacgaaggc cgggaggatg tacaggtacg gccgcgccgc gtggagcata cgccgccaga
     4261 cccactcggg tccccgacag ccggccgcct ccactccggt gagagggatt gcccccatct
     4321 tggatccgga tgagcggttc acacatcact ccttcgggcc aagaacggct cccgattgaa
     4381 cattgtcata atcgtgtgtg gagttcaagt gcggttatac ctgggtagga tctccaagga
     4441 tggcgagggc ccggatgggt gatccgtcca ggcccgtgag ggcaagcggc agacccacga
     4501 agaagaccag gggctgactc aggctcgaga ggtggatcag accttccgtg acggccactc
     4561 cgccacggaa caggatct
//
LOCUS       DBNGZC010000204         1094 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_408023, whole genome shotgun sequence.
ACCESSION   DBNGZC010000204 DBNGZC010000000
VERSION     DBNGZC010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_408023"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..726)
                     /locus_tag="AC16G0_03755"
     CDS             complement(208..726)
                     /locus_tag="AC16G0_03755"
                     /inference="COORDINATES: protein motif:HMM:NF025819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HIR5098770.1"
                     /translation="MNQDLRATSHMLPGLTPVHLAEIARQAEAGYPEEICGIVIGKRG
                     APDTYQIRQVRNLANQERQRDTGGVLRNARTAYFGDAREMLRISTEADLHGWETLVLY
                     HSHPDHDAYFSAMDRERALWPGQEPPEPLWPGVSYLVVAVRKGRAWEARYHVWDEAGR
                     DFVEVPVSLPSL"
     gene            complement(790..>1094)
                     /gene="cysM"
                     /locus_tag="AC16G0_03760"
     CDS             complement(790..>1094)
                     /gene="cysM"
                     /locus_tag="AC16G0_03760"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743596.1"
                     /note="catalyzes the formation of cysteine from
                     3-O-acetyl-L-serine and hydrogen sulfide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HIR5098771.1"
                     /translation="RSLEEGFIPPILDTSLLDGKIIVNSRCAFAATKDLTSKEGIFAG
                     VSSGAVIHCAVRLAHGMERGTIVCILCDGGWKYVSLGVWFKEFPDLGENVQSHIWW"
BASE COUNT          199 a          377 c          317 g          201 t
ORIGIN      
        1 cgttttcatc cggttcctcc ccccgaccac gtggccttgg ccatccgcat gggcgccttc
       61 accatatccc aagacgtctc tcctacaaag cctctttcga acacctgcat ctcccctggg
      121 agcggcggcg gtcctcgttg ctcttcgcac gcagggagca gagactaggc ggaggaagat
      181 cgggcaggcg ctatgacggc cagcagatca gagagacggc aggctcaccg ggacctccac
      241 gaaatccctc ccggcttcat cccagacatg gtaccgggct tcccaggccc gaccctttcg
      301 caccgcaacg acgagatagc tcacgccggg ccaaaggggc tccggaggct cctggcctgg
      361 ccacaaggct cgctcgcgat ccatggcgga gaagtaggcg tcatgatccg ggtgggagtg
      421 atacagcacc agggtttccc agccgtggag gtcggcctcg gtggagattc gcagcatctc
      481 gcgcgcgtct ccgaaatagg ccgtccgggc attgcgaagg acgcccccgg tgtcacgctg
      541 gcgctcctga ttggcaaggt tcctcacctg acgaatctgg taggtgtcgg gtgcgccccg
      601 cttcccgatg acaataccgc agatctcttc cggatacccc gcctcggcct gacgggcgat
      661 ctcggctaag tggaccggcg tcagccccgg cagcatgtga ctcgtggctc ggaggtcttg
      721 gttcatagcc cttgccatcc gccaacagcc ttgcacgccc ttggggcgct cggcctatcg
      781 ctcgccagat caccaccaga tgtgactctg gacattctcc cccaggtccg gaaattcctt
      841 gaaccagacc cccaaactca cgtacttcca gccgccgtca cacaggatgc aaacgatcgt
      901 ccctcgctcc atgccgtggg ccagccggac ggcgcagtgg atcaccgctc ccgaagaaac
      961 cccggcgaaa atcccctcct tgctggtcaa gtccttggtg gctgcgaagg cgcatcggct
     1021 gttgacgatg atcttgccgt ccaggaggct ggtatccagg atggggggga tgaacccctc
     1081 ttccagactc cgca
//
LOCUS       DBNGZC010000205         2667 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_603553, whole genome shotgun sequence.
ACCESSION   DBNGZC010000205 DBNGZC010000000
VERSION     DBNGZC010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_603553"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="AC16G0_03765"
     CDS             complement(<1..395)
                     /locus_tag="AC16G0_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014242636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HIR5098772.1"
                     /translation="MSKLDELRRGAGGNAAESMGAGVARRSLAEGVSNPIGGARYKDL
                     AKTKNAFDVPVDKIVPDPNQPRKVFTPEDLQDLSDSIRARGLLQPIRVRWDEGREKYV
                     IVAGERRWRAAIMAGKATITCVIVEGEMT"
     gene            complement(395..1192)
                     /locus_tag="AC16G0_03770"
     CDS             complement(395..1192)
                     /locus_tag="AC16G0_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR5098773.1"
                     /translation="MLLNQKGGVGKTSTCHHLAGTLAKGGKKILLVDNDPQASLTQGL
                     FGPQTTRDLDPRSTIAAVYSGPAVAEQIIHRTEISGINLVPGSRYAMKYNNAEPWAAD
                     RDLQIGLRDVIEAVRDRYDVVLIDCPPNLHLCSWAALVASDHLIVPLQPEDYGAQGII
                     DVQESVALVTELANPGLSLLGYLITMFNARKSIHKMYEETLRAQYGPDVFATRIPHAA
                     EFPEAIAFRKPVAQYKPKGAAAKAIQALADELQGRLDARRTHLGEAA"
     gene            complement(1468..2613)
                     /locus_tag="AC16G0_03775"
     CDS             complement(1468..2613)
                     /locus_tag="AC16G0_03775"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HIR5098774.1"
                     /translation="MDGQRTTIVVKRGGIWRRVTMRPYSRDLRERIAAAVDERDGSQR
                     QIARRFRVSLSFVARLLRRRRQTGSVDPQPHGGGHPPAFQGTDRERLRQLVQEQPDAT
                     LDELRQRLGVPCSLTAIWRALRPLDLPRKKKVLRARQQGDPKVQEKRRAFQEKVAGID
                     PQRLVFVDESGATTAMTRRYGRAPRGERVIGEVPGQWKSVTLISAMRLAGVGASLAFE
                     GATDTPAFQTYVEEMLVPQLCPGDVVIWDNLKPHQDEQVRRAVEQAGATVEPLPPWSP
                     DLTPIEKMWSKVKEFLRSVAARTTEAVYDAMGQALRAVRSRDIVGWFGSCGLGVSEGL
                     KFAAPQRSGDRRRAGSDRGARPEGEHPPPRLLDHGWFPSWSLCATQA"
BASE COUNT          443 a          880 c          862 g          482 t
ORIGIN      
        1 tcggtcatct caccctcgac gatgacgcag gtgatcgtcg ccttacccgc cataatcgcg
       61 gcccgccacc gccgctcacc ggccacgatg acgtacttct ctcgcccctc gtcccaacgc
      121 acacgaatcg gctgcaagag gccccgggcg cgaatcgagt cggataggtc ttggaggtcc
      181 tcgggtgtga agaccttccg gggctggttg gggtcgggca cgatcttgtc gacggggaca
      241 tcgaaggcgt ttttggtctt cgcgaggtcc ttgtaccggg cgccgccgat tgggttcgac
      301 acgccctccg ccagggaccg gcgggcgacc ccggccccca tactctcggc ggcgttgcca
      361 ccggccccgc ggcgcagttc gtcgagcttg ctcatcaggc agcctccccg aggtgggtac
      421 gtcgggcgtc gaggcgtcct tgaagttcgt cggccagcgc ctggatcgcc ttggcagccg
      481 cccccttggg cttgtactgg gcgactggct tgcggaaggc gatcgcctcc gggaactcgg
      541 ctgcgtgggg gatgcgcgtg gcgaagacgt cgggcccgta ctgtgcgcgg agcgtctcct
      601 cgtacatctt gtgaatcgac ttgcgggcgt tgaacatggt gatcaagtag cccaggagcg
      661 acaggcccgg gttagccagt tcggtgacca gcgcaaccga ttcctgcacg tcgataatac
      721 cttgggcacc gtagtcctcg ggctggagcg gcacgatcag atggtcgctg gcgaccagcg
      781 cggcccaaga gcagaggtgg agattcggtg ggcagtcgat cagaaccaca tcgtagcggt
      841 cgcgtacggc ctcgatgacg tcgcggaggc cgatctggag atcgcggtct gcggcccatg
      901 gctccgcatt gttgtatttc atggcgtacc gcgagccggg taccaagttg atcccggaaa
      961 tctccgtgcg atggataatc tgttcggcga cggcgggacc agagtagacc gccgcaatgg
     1021 tggatctagg gtcgaggtca cgggtggtct gtggcccaaa cagcccttgg gtaaggctgg
     1081 cctgcgggtc gttgtcgacc aagaggatct tcttcccccc tttggcgagc gtgccagcca
     1141 agtggtggca ggtcgaggtc ttcccaaccc cgcctttttg gttcagaagc acgatggaga
     1201 cggcaggcat agggtttcac ttcccgggga agcttggtga gggcgtccga cagcgccgga
     1261 cagacagacc cgcggccggc gcacttcccc gggaaggacc gacccttttt cactatcggc
     1321 ggccaagtcg attcggatac ggtattttcc cttcccgggg aaggatgata ccgtgcgacg
     1381 attccaagct aatccggcag agatgggtct ctgtcccagt aggatgacgc tgcgaagggt
     1441 gaagcatagg aggagagcta gagcgcttta cgcttgggtt gcgcacaagc tccaggatgg
     1501 gaaccagccg tgatcgagca gccggggtgg cggatgctct ccctcagggc gtgcgccccg
     1561 gtcactgccg gcgcgccggc gatcgcccga acgctgcggg gcagcaaact tcaacccctc
     1621 gctgacaccc aagccacacg agccgaacca gcccacgatg tcacgcgaac ggaccgcgcg
     1681 cagcgcctga cccatcgcgt catagaccgc ctcggtcgtc cgcgccgcta ccgaccgcag
     1741 aaactccttg accttcgacc acattttctc gatcggcgtc aggtccgggc tccacggcgg
     1801 cagcggctcc accgtcgcgc cggcctgctc caccgcacgt cggacctgct catcctgatg
     1861 cggcttcagg ttgtcccaga tcaccacatc ccccggacac aactggggga cgagcatctc
     1921 ttccacgtag gtctggaacg ccggagtatc ggtggccccc tcgaacgcca gcgacgcccc
     1981 cacccccgct agccgcatcg ccgagatcag tgtcaccgac ttccactggc ccggcacctc
     2041 cccgatgacg cgctcgcccc ggggggcgcg gccgtagcgc ctggtcatcg ctgtcgtggc
     2101 cccgctctca tcgacgaaga ccaggcgctg cgggtcgatc ccggccacct tctcctggaa
     2161 cgcccggcgt ttctcctgga ccttgggatc gccctgctgg cgggcccgca agaccttctt
     2221 cttgcgcggc aagtcgagcg gccgcagggc gcgccaaatc gccgtgaggc tgcatggcac
     2281 gcccaggcgt tggcgcagtt cgtccagggt ggcatcaggc tgctcctgga ccagttggcg
     2341 caaccgttcg cggtccgtcc cctgaaaggc cggcggatgt ccgcccccat ggggttgggg
     2401 gtccacggag ccggtctgtc tgcggcggcg gagcagacga gcgacgaacg acaggctgac
     2461 gcggaagcgc cgtgcgatct gccgctgcga gccgtcgcgt tcatcgacag cggccgcgat
     2521 acgctcgcgg agatccctcg aatagggtct catggtaact ctcctccaga tacctcctct
     2581 ctttactacg atggtggtgc gttgtccgtc caaacgaaaa gcgctctagc cgcatacagt
     2641 cggagaacgt tggacaatcc ttctcgt
//
LOCUS       DBNGZC010000206         2260 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2462886, whole genome shotgun sequence.
ACCESSION   DBNGZC010000206 DBNGZC010000000
VERSION     DBNGZC010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2462886"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..1001
                     /locus_tag="AC16G0_03780"
     CDS             228..1001
                     /locus_tag="AC16G0_03780"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR5098775.1"
                     /translation="MPPIPAKPEMSPGEEREERLQKFLARAGVASRRECETYITAGRV
                     QVNGRVVTTLGTRMRPGRDRVKVDGQPVRTEAAVTLMLHKPSGYITSLTDPQGRPVVM
                     SLLPQQGMPRLFPVGRLDWDTEGLLLLTNDGELANLLTHPRHGVRKVYHAKLKGHMSF
                     ESLGRLLHGVMCEGERLFAASVEMMHPTQENTWVAIGIEQGRYRQVRRMCEAIGHPVQ
                     KLIRVALGPISLGSLPRGQWRPLTLAELGSLQALQKQRP"
     gene            1056..2135
                     /gene="pheA"
                     /locus_tag="AC16G0_03785"
     CDS             1056..2135
                     /gene="pheA"
                     /locus_tag="AC16G0_03785"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR5098776.1"
                     /translation="MRIADLRAKIDEIDSQILQLLNRRAQLAIEIGRIKAEQNLTFHV
                     PQREEEIYTRLAAANTGPFPSQALRPVYREVISACLSLEHPLRVAYLGPPATFSHLAA
                     MERFGLSAEFLAMRSIAEVFAEVEKANADFGVVPVENSTEGVVSHTLDMFVDSGLLIC
                     GEVVVEATLHLLSKAGSLGEVRQIYSHPHALAEARKWLEQNSPRIPVVETSSTSAAAE
                     TAASEPGAAAIASELAANLYGLRVLQRRIEDHPNNLTRFLVIGRKAAPPSAADKTSIL
                     FSIKDRVGTLHRMLQPFAEQEINLTKIESRPSKKKVWEYIFYVDFEGHASEARIQRAL
                     EALKDECVFLKVLGSYPKTHLPKEG"
BASE COUNT          402 a          768 c          737 g          353 t
ORIGIN      
        1 cgtcccgtcc ccctcgggag atgggtccgg cgaggccaag ggcggacagc cggtctgagg
       61 actcgtttcc tcggaacgag cgccgtccat cgcgagtcgg ccgcccgccc gcgccgcctc
      121 gtcggggcgg acgggtcgcg acgaatccag atcgctggcg acctgcctcg agtctccagt
      181 tggttgcgcg accccggggt ggagaccgtt cgagcgcgcc ctcgagggtg ccgccaattc
      241 ctgcgaaacc ggagatgtcc cccggcgagg agcgcgaaga acgcctgcag aagttcctgg
      301 ctcgggcggg cgtggcctcc agaagggagt gcgagacata catcacggcc ggccgcgtcc
      361 aggtgaacgg ccgcgtggtg acgacgctgg ggacgcgtat gcgacccggt cgggatcggg
      421 tcaaagtgga cggtcagccg gtccggaccg aggcggcagt caccctcatg ctgcacaagc
      481 cgtccggcta catcacctcg ctgaccgacc cacagggccg cccggtggtg atgtcgctcc
      541 tgccgcagca ggggatgccg cggctgttcc cggttggcag gctggactgg gataccgagg
      601 gcctgctcct cctgaccaac gatggggagc tggcaaacct tctgacccat ccgcggcatg
      661 gtgtgcgcaa ggtctaccac gccaagctga aggggcacat gagcttcgag agtctgggcc
      721 gcttgctgca cggggtaatg tgcgagggcg agcgcctctt cgccgcctcg gtggagatga
      781 tgcacccgac ccaggagaac acgtgggtgg ccatcggcat cgagcaggga cgctaccggc
      841 aggtccgtcg catgtgcgag gccatcggcc atccggtcca gaagctcatc cgggtggcct
      901 tggggccgat cagcctgggg tcgctcccac gcggccagtg gcgccccctc acgctggccg
      961 aactcggcag cctgcaggct ctgcagaaac aaagaccttg atcggcccgc accccccgac
     1021 tatcatgcgg tagagatgaa ctgacgaggc cccgaatgcg gattgccgac ctgcgcgcaa
     1081 agatcgacga gatcgactcc cagatcctcc aactcctgaa ccgccgggcg cagttggcga
     1141 tcgagatcgg tcgcatcaag gcggagcaga acctcacctt ccacgttccg cagcgtgagg
     1201 aggagatcta cacgcgtctg gccgcggcca acacagggcc ctttccctct caggcgctgc
     1261 gcccggtata ccgtgaggtg atctcggcct gcctctccct ggagcatccc cttcgcgtgg
     1321 cctacctggg gcctccggcc accttctccc acctggccgc catggagcgc ttcggtctct
     1381 ccgccgagtt cctggccatg cgcagcatcg ccgaggtgtt cgccgaggtg gagaaggcca
     1441 acgccgactt cggcgtggtc ccggtggaaa actccaccga gggtgtggtg agtcacaccc
     1501 tggacatgtt cgtggactcc ggcctcctga tctgcgggga ggtggtggtg gaggccacgt
     1561 tgcacctcct gagcaaggcc ggcagcctgg gagaggtccg ccaaatctac tcgcaccccc
     1621 acgccttggc ggaggctcgc aagtggctgg agcagaattc gccgaggatc cctgtggtgg
     1681 agacaagcag caccagcgct gccgccgaga ccgccgcctc ggagccgggc gcggcggcca
     1741 tcgcctcgga gttggcggcg aatctctacg gcctgcgcgt cctccagcgg cgcatcgagg
     1801 accacccaaa caacctgaca cgcttcctgg tcatcggccg gaaggccgcc ccgcccagcg
     1861 cggccgacaa gacgtccatc ctgttctcca tcaaggaccg ggtggggacg ctccaccgca
     1921 tgctccagcc cttcgcggag caagagatca acctgaccaa gatcgagtcc cggccctcca
     1981 agaagaaggt gtgggagtac atcttctacg tcgatttcga agggcacgcc agcgaggccc
     2041 gcatccagcg cgccctggag gccctgaagg acgagtgcgt cttcctgaag gtcctggggt
     2101 cgtatcccaa gacccacctc cccaaggagg gctaacccgg attactcccg aactccttcg
     2161 cgaatagacc gggataatga ccaaatccca atgatcaatg attaacacag gtagcactcg
     2221 gggaacgtca ctcggcggcg cgctccgcgc gccggctcaa
//
LOCUS       DBNGZC010000207         2496 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_311066, whole genome shotgun sequence.
ACCESSION   DBNGZC010000207 DBNGZC010000000
VERSION     DBNGZC010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_311066"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="AC16G0_03790"
     CDS             complement(<1..224)
                     /locus_tag="AC16G0_03790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5098777.1"
                     /translation="MTKHERILIVDDEEQMRDLLVKVLEKNGFQVAVCGGGSEALAFL
                     EKEPMDLVVTDVRMPGMGGMEALRAIKELNP"
     gene            complement(221..2050)
                     /locus_tag="AC16G0_03795"
     CDS             complement(221..2050)
                     /locus_tag="AC16G0_03795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5098778.1"
                     /translation="MGLGAKLTLWVLVPLLVVLILYAAITLKREQEVHQQEVGDEAER
                     IANTLAIAVAESLRRQNPENLPSIAERSALTPDHFGLAVYDSSGRPVMTWGLAAKTGA
                     VGRQELTALSSLPRGLGLVEEEQGVPVRSHLVALMAGGELLGGLRVSVSLQEIQETLT
                     RERDYFLSMLAVVTVVLVVLISVTIRRTVSLPLAGLMARVADLGRGQLPEDVVVPGRD
                     EVARLAQEFNALAKSLDEARQRLVRESEHARNVIQSITDGIIGLDAACRIRTWNRAMA
                     DRYEIPEAEVLGRDLFEAFPLLGQEGLRKEVDLLLSGKSRDFSLRYFEHETLRRGRVV
                     LNIRGSALLGATDEIAGAVLAIEDVTDRVSMAQEVQQAEKLAVVGQLAAGIAHQIGTP
                     LNVISGSAEYLMMEWGAERPRPRELDIIVAQTDRITKLIQQLLNFARPARMELEVLKL
                     DDLLREVLGLIGHQIAKEKITIHTDLQPDLPPITGDANQLEQAFLNIVINAWHAMPDG
                     GTLTFRTRLVPPIDRGRRFGRAGTPGVEVIISDTGTGIPLEQLPRIFDPFFSTKGVGK
                     GTGLGLAISRRIVEDHHGSIEVTSEVGCGTTFTIWLPAGGSAA"
BASE COUNT          429 a          833 c          766 g          468 t
ORIGIN      
        1 gggttcaact ccttgatggc ccggagcgct tccatgccgc ccatcccagg catgcgcacg
       61 tcggtgacca ccaggtccat cggctccttc tccaggaagg ccagggcttc gctcccgccc
      121 ccgcacacag cgacctggaa gccgttcttc tccaggacct tcaccagcag gtcccgcatc
      181 tgctcttcgt cgtccacgat caggatacgc tcatgtttcg tcatgccgcc gatccccccg
      241 ctggcagcca gatggtgaag gtggtcccgc accccacctc gctggtcacc tctatacttc
      301 cgtgatggtc ctccacgatc cgccggctga tggcgagccc cagcccggtt cccttgccga
      361 cccctttggt actgaagaag ggatcaaaga tccggggaag ctgctccaat gggatcccgg
      421 tcccggtgtc cgagatgatc acctcgacgc ccggcgttcc agccctcccg aatcggcgcc
      481 cgcgatcgat cggcgggacc aggcgcgtgc ggaaagtgag cgtccccccg tcgggcatgg
      541 catgccaggc gttgatcacg atgttcagga aggcttgctc cagttgattg gcgtctcccg
      601 taatgggcgg gagatccggt tgaaggtcgg tatggatggt gattttttcc ttggcgatct
      661 ggtgcccaat gaggccaagg acctcgcgca gcaggtcatc cagcttcagc acttccagtt
      721 ccatgcgggc gggccgggcg aagttcaata gctgctggat gagcttggtg atgcggtccg
      781 tctgggccac gatgatgtcc aactcgcgcg ggcggggtcg ctcagccccc cattccatca
      841 tcaggtactc ggcggagccg gagatcacgt tgagcggcgt cccgatctga tgcgcgatcc
      901 ccgcggcgag ttgccccacc acagccaatt tctcggcctg ctgcacctcc tgcgccatgg
      961 acacgcggtc ggttacgtcc tcgatggcca ggaccgcgcc cgcgatctca tcggtcgccc
     1021 ccaacagggc gctcccccgg atgttcagaa ccacccggcc gcggcgcaag gtttcgtgct
     1081 cgaagtagcg gaggctgaag tcccgggatt tcccgctgag caggaggtcc acctccttgc
     1141 gcaggccctc ctgccccaag agggggaagg cttcgaacag atcccggccc agcacctcgg
     1201 cctcggggat ttcatagcgg tcggccatgg cccgattcca cgtccggatc cggcaggcgg
     1261 catcaagtcc gatgatgccg tcggtgatgc tctggatcac atttcgcgcg tgctcgctct
     1321 cccggacgag tcgttggcgg gcctcgtcca ggctcttggc cagggcgttg aattcctgcg
     1381 ccaggcgcgc cacctcgtcc cgacctggaa ccaccacgtc ctcgggcaat tgacctcgtc
     1441 cgaggtccgc gacgcgcgcc atgaggcccg ccaggggcag gctgactgtg cgtcggatcg
     1501 tgacggagat caggacgacc agaacgacag tcaccaccgc cagcatgctg aggaaataat
     1561 cccgctctct ggtcagcgtc tcctggattt cctgcaggct gacggatacc cggagacctc
     1621 ccagtaactc gccgcccgcc atcagggcaa ccaggtgact ccgcaccggg acgccttgtt
     1681 cctcctctac caagcccagg cctcgaggga ggctggagag ggcggtcaac tcctgccgcc
     1741 cgacagcgcc ggtcttcgcg gccagacccc aggtcatgac cgggcggccg gatgaatcgt
     1801 acacggccag gccgaagtga tccggtgtga gggcggagcg ttcggcgatg ctggggaggt
     1861 tttccgggtt ttggcgtcgg agagactcgg cgaccgcgat cgccaaggtg ttggcgatac
     1921 gctccgcctc atcccccacc tcctgctggt gcacttcctg ctcccgcttc agggtgatgg
     1981 cggcatacag gatcaacacc acaaggagtg ggacgagaac ccacagggtc agtttggcgc
     2041 ccaggcccat tggacaactc tcttagagga gtgtctcggc aggtaagtgc cttgctcacg
     2101 atcaggctgt attcgaatca attcgatcca attctcgggt ctagtggctc tatcgagacc
     2161 tactatagct gagtcgtccc actgccgcaa tgagacgggc aggattgtct catactggac
     2221 acaatgcccc gtcttcagac aacagtgaag ggcaggagat cgaatgagtc tcgtctgtga
     2281 cctagcgtcc ggcggtatcg aggtccggca attcgtcgac gtacctcgag gacggggcac
     2341 tagtgtcctg tccctgaagt tgaccacata attgaggccg ttccggctcg ccctgagcct
     2401 gccgggcccg cgggccagcc ggcttcggga cgccgtggct gcggaagcat cgaactgccc
     2461 aggaaggctg cgcccacggc gctacgcgcc ccgcag
//
LOCUS       DBNGZC010000208         4378 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1387037, whole genome shotgun sequence.
ACCESSION   DBNGZC010000208 DBNGZC010000000
VERSION     DBNGZC010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1387037"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1559
                     /gene="dop"
                     /locus_tag="AC16G0_03800"
     CDS             75..1559
                     /gene="dop"
                     /locus_tag="AC16G0_03800"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HIR5098779.1"
                     /translation="MAIEKIMGSETELGITTRDAAGFDPVGSSIFLINALPVGPPPRA
                     MWDYAGENPLLDARGFEVSGERERPSQQDNRAINKVLLNGGRLYVDGAHPEYSTPETT
                     NARDLVLFEKAGERLVDRCRAAANQKLPPAQQLLVYKNNSDGKGNSYGYHENYLVARS
                     VPFERLVDGLTPFLVSRIVICGAGKVGAENSTEPCEYQISQRADFFETLIGLDTMAKR
                     PIINTRDEPHADEEKYRRLHVIVGDANMSEVTTYLKVGITAIVLAMVEDGVLRPDLAL
                     EDPVRAIKEISHDPTCRRKVRLKRGAEVSAVELQREYLACALDYYATAEQSPQVSDLL
                     ERWQSVLDRLAEDPRACARELDWVIKQELITSYRTRKDIGFADQRLSLLDLQYHDLRQ
                     DKGLYYTLERQGYVDRLLGDAEILQAMTQPPTDTRAYFRGMCLQKFPEHTYGASWSSI
                     LLDTGESTVKKIPMAEPGRGTRKLVGDILERSESVAELLERLAG"
     gene            1574..2374
                     /gene="prcB"
                     /locus_tag="AC16G0_03805"
     CDS             1574..2374
                     /gene="prcB"
                     /locus_tag="AC16G0_03805"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005267374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HIR5098780.1"
                     /translation="MDFGLNSLGAGSSFFDMVRQQEPSLLPQFALEGLEAKAAVPPIP
                     VGTTILALRYQEGVLIAGDRQATEGYQVAFRRIEKVYATDDHSAMAIAGAAGPSIEMV
                     RLFQTELAHYEKLEGMVLSLEGKANKLAQMIRANLPMAIQGLVVVPIFVGYDLKRDEG
                     RIYKYDVAGGRYEETDYYATGSGGKDARSSLKKLYRAGMAEGEAVHLALEALYDAADE
                     DTGTGGPDFVRKIFPTVKVVSRTGIRDIPEDRVAAECEAIVTARKSAS"
     gene            2443..3144
                     /gene="prcA"
                     /locus_tag="AC16G0_03810"
     CDS             2443..3144
                     /gene="prcA"
                     /locus_tag="AC16G0_03810"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019773 - proteasome core complex,
                     alpha-subunit complex [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HIR5098781.1"
                     /translation="MPLPYYVSPEQMMQDKAEYARKGIAKGKSIIALEYQDGVLIVAE
                     NPSASLNKISEIYDRIAFAGAGKYSEFERLRKEGIRYADLKGYAYGREDVTAKSLANA
                     YSQVIGNIFSMEIKPLEVEILVLEVGETPETNELYRIAFDGSIADERGYATIGGQAEE
                     LKLFLRDKSVQNPNLEGALRLATRALEAAASQKVKAAILEVAVLERGRVGRKFRRLSP
                     PEVQALIEKEGDGAA"
     gene            3262..>4378
                     /locus_tag="AC16G0_03815"
     CDS             3262..>4378
                     /locus_tag="AC16G0_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HIR5098782.1"
                     /translation="MQRRIFGLENEYGLISSSAGGRVNLSVESALGYLFEKVVSRQRG
                     TNDFLRNGARLYQDTGCHPEYATPECDNALDLTIHDKAGERIIEELLLTAERKLHENG
                     IDCDIFIFKNNTDSVGNTYGCHENYLVERGINFHKLAEQLIPFFVTRQVFAGAGKVLK
                     TRLGNHYYISQRAQHIYQEISGATTSSRGIINTRDEPHADEEKYRRLHVIVGDSNMSE
                     VTTYLKVGTTAIVLAMVEDGFIRKDLALEDPVRAIKEISHDTTCRRKVRLKRGKEYSA
                     VELQREYLDLALDYYAAAEQSPQVSDLLERWQSVLDRLAEDPMACARELDWVIKRELI
                     NSYMGRKDIGFDDQRISMLDLQYHDIRQDRGLYCMLCR"
BASE COUNT          858 a         1408 c         1435 g          677 t
ORIGIN      
        1 gcggcgtctc gttcggagcg ccgtgcggcc gcctggcctt attcgccgcg ccgccgcact
       61 gaaaggatag ttgcatggcc atcgagaaga tcatggggag cgagacggag ctggggatca
      121 cgaccaggga cgccgcgggc ttcgatcccg tgggaagctc catcttcctc atcaatgccc
      181 tgcccgtggg gccgcccccg cgggccatgt gggactacgc cggagaaaac ccgctcctgg
      241 acgccagggg cttcgaggtg agcggcgagc gggagcgccc cagccagcag gacaaccggg
      301 ccatcaacaa ggtgctcctc aacggcggcc ggctgtacgt ggacggggcc caccccgagt
      361 actcgacccc cgagaccacc aacgcccgcg acttggtgct cttcgagaag gccggggaac
      421 ggctcgtcga ccgctgtcgc gctgcagcca atcagaaact gccacccgcc cagcagcttc
      481 tggtctacaa gaacaacagc gatgggaagg ggaacagtta cgggtaccac gagaattacc
      541 tggtggcgcg gagcgtcccc ttcgagcggc tggtggacgg gctcaccccc ttcctggtct
      601 cccgcatcgt catctgcggc gcggggaagg tgggggcgga gaacagcacc gagccgtgcg
      661 agtaccagat ctcccagcgg gccgatttct tcgagacgct gatcggtctg gacaccatgg
      721 cgaagcggcc gatcatcaac acccgggacg aaccccacgc cgatgaggag aagtaccgcc
      781 gtctccacgt catcgtcggc gacgccaaca tgtccgaggt cacgacctac ctcaaggtgg
      841 ggatcacggc catcgtcctg gccatggtgg aggatggcgt tctccggccg gatctcgccc
      901 tggaggaccc ggtgcgggcc atcaaggaga tctcgcacga ccccacctgc cggcggaagg
      961 ttcgcctcaa gcggggggcc gaggtttccg ccgtggaact ccagcgggag tacctggcgt
     1021 gcgccctgga ctactacgcc acagccgagc agtcccctca ggtgagcgac cttttggagc
     1081 gctggcagag cgtcctggat cggctggccg aggatccccg ggcctgcgcc cgagagctgg
     1141 actgggtcat caagcaggag ctcatcacct cctaccggac ccggaaggac atcggcttcg
     1201 ccgaccagcg gctcagcctg ttggacctgc agtaccatga cctccgccag gacaaggggc
     1261 tctactacac cctcgagcgg cagggatatg tggaccgact gctgggcgac gccgagatcc
     1321 tgcaggcgat gacccagccc cccaccgaca cccgcgccta cttccgcggg atgtgtctcc
     1381 agaagttccc cgagcacacc tacggcgcga gctggagctc catcctcctg gacacgggcg
     1441 aatccacggt gaagaagatc cccatggccg agccagggcg gggcacgcgg aagctcgtgg
     1501 gggacatctt ggagcggtcg gagagcgtgg ccgaactctt ggagcgcctg gcaggctgag
     1561 gagggcctca ccggtggact tcggactgaa ctctctgggt gcgggatcca gcttcttcga
     1621 catggtgcga cagcaggagc cgtccctctt gccgcagttc gccctcgagg gactggaggc
     1681 gaaggcggcg gtgcccccga tccccgtcgg gaccacaatc ctggcactcc gctaccaaga
     1741 gggtgtgctg atcgcagggg accggcaggc cacggaaggc tatcaggtcg ccttccggcg
     1801 gatcgagaag gtgtatgcca cggacgatca ttcggccatg gccatcgccg gcgccgccgg
     1861 tccgtccatc gagatggtcc gcctgtttca aacggaactg gcccactatg aaaagctgga
     1921 gggcatggtc ctgagcttgg agggaaaagc gaacaagctg gcccagatga tccgagccaa
     1981 tctgccgatg gccatccagg ggctggtggt ggtcccgatc tttgtgggct acgatctgaa
     2041 gcgggacgag ggccgaatct acaagtacga cgtggccggt ggccggtacg aggagacgga
     2101 ttactacgcc accggctccg gaggcaagga tgcccgctcc agcttgaaaa agttgtaccg
     2161 ggcggggatg gccgaaggcg aggccgtcca cctggccctg gaggcgctct acgatgccgc
     2221 ggacgaggat accggcaccg gcgggccgga ttttgtccgg aagatcttcc ccacggtcaa
     2281 ggtggtcagc cggaccggga tccgggacat cccggaggac cgtgtggcgg ccgagtgtga
     2341 ggccatcgtg accgcgcgca agtctgcctc ctagggacac aatccggcgc tcccggcccc
     2401 agccatggcg gccgggcggc tcccattgcg aaggatacac ccatgccgct cccctattac
     2461 gtctcgcccg agcagatgat gcaggacaag gcggaatacg cccgcaaggg catcgccaag
     2521 ggcaagtcca tcatcgccct ggagtaccag gacggcgtcc tgatcgtggc agagaatccc
     2581 agcgcctccc tgaacaagat ctcggagatc tacgaccgga tcgccttcgc cggggccggc
     2641 aaatacagtg agttcgagcg gctacggaag gagggaatcc ggtatgccga cctgaagggc
     2701 tacgcctacg gccgcgagga cgtgacggcg aaatccctgg ccaacgcata ctcgcaagtg
     2761 atcgggaaca tcttcagcat ggagatcaag ccgctggagg tggagattct ggtcctggag
     2821 gtgggcgaga cgcccgagac caacgagctg taccgcatcg ctttcgatgg aagtatcgcg
     2881 gacgagagag gctatgccac catcgggggc caggccgagg agttgaagct cttcttgcgg
     2941 gacaagtccg tgcagaatcc gaatctggag ggggcgcttc gtctggccac tcgggcgctg
     3001 gaagcggccg ccagccagaa ggtgaaggcg gcaatcctgg aggttgcggt tctcgaacgc
     3061 ggtcgagtag ggcggaaatt ccgtcgcctc tccccgccgg aagtccaggc cctcatagaa
     3121 aaggaggggg acggggccgc ctaaggcggc tccgcgatgt gcctgtcggt accccatgga
     3181 accgccgacg ccctactgag atcattccgg gttgtcgcgg ggacgagcgg tcgtcgccag
     3241 gccctcgaat tcccggccgc catgcagcgg cggatctttg gtctcgaaaa cgagtacggt
     3301 ctgatctctt cctcggccgg gggacgggtg aacctctccg tggagagcgc tctgggctac
     3361 ctgtttgaga aggtggtctc ccggcagcgc ggcaccaacg acttcctcag aaacggcgcc
     3421 cgcctgtacc aggataccgg ctgtcacccc gaatacgcca cccccgagtg cgacaacgcg
     3481 ctcgatctca cgatccacga caaggccggg gagcgcatca tcgaggaact cctgctcacc
     3541 gcggagcgca agctccacga gaacgggatc gactgcgaca ttttcatctt caagaacaac
     3601 acggactccg tgggcaacac ctacggctgc cacgaaaact atctggtgga gcggggaatc
     3661 aacttccaca agctggcgga gcagctcatc cccttcttcg tgacccgcca ggtctttgcc
     3721 ggggccggca aagtcctgaa gacgcgcctg gggaaccact actacatctc gcagcgcgca
     3781 cagcacatct accaggagat ctcgggcgcc accaccagct cccgcggcat catcaacacc
     3841 cgggacgagc cccacgccga tgaggagaag taccgccgcc tccacgtcat cgtcggcgac
     3901 tccaacatgt ccgaggtcac gacctacctc aaggtgggga ccacggcgat cgtcctcgcc
     3961 atggtggagg acggcttcat ccggaaggac ctcgccctgg aggacccggt gcgggccatc
     4021 aaggagatct cgcacgacac cacctgccgt cgaaaggtcc gtctgaagcg agggaaggag
     4081 tactccgccg tggagctcca gcgggagtat ctggacctgg ccctggacta ttacgccgcg
     4141 gccgaacagt ccccccaggt gagcgacctt ttagagcgct ggcagagcgt cctggaccgg
     4201 ctggccgagg atccaatggc ctgcgcgcgg gaactggact gggtcatcaa gcgggagctc
     4261 atcaactcct acatgggccg gaaggacatc ggattcgacg accagcggat cagcatgctg
     4321 gacctgcagt accacgacat ccgccaggac cggggcctgt attgcatgct ctgccggc
//
LOCUS       DBNGZC010000209         2148 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1581466, whole genome shotgun sequence.
ACCESSION   DBNGZC010000209 DBNGZC010000000
VERSION     DBNGZC010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1581466"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..962
                     /gene="proC"
                     /locus_tag="AC16G0_03820"
     CDS             147..962
                     /gene="proC"
                     /locus_tag="AC16G0_03820"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR5098783.1"
                     /translation="MLQGRRLGFVGGGNMGEALLRGLLAAKIVGPDSLLVSDAREDRL
                     TSLRQAYGVRTHSSNAEVARQADILLLAVKPQVIGQALDDLVEALDEKKLVISIAAGI
                     PTSFILDRFSFPLRVVRVMPNTPALVLEGMSALSLGVHATAEDLETTRLLFETVGKVV
                     VVDESLLDAVTGLSGSGPAYIFLVIEALADAGVKVGLPRDVAHLLAAQTVRGAARMAL
                     ETGKHPGQLKDMVTSPGGTTIAGLHALEQGGVRAAFFNAVEAATRRSRELGRR"
     gene            1003..1302
                     /locus_tag="AC16G0_03825"
     CDS             1003..1302
                     /locus_tag="AC16G0_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017951881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR5098784.1"
                     /translation="MFVAGNFLDAVAAVLNLALWAYMWIIIARALISWVNPDPWNPIV
                     QFLQRATEPVLAPIRRRLPSWQWGIDFSPLVVILVIYFLQAWLVGSLRDLAWRMR"
     gene            1302..1766
                     /locus_tag="AC16G0_03830"
     CDS             1302..1766
                     /locus_tag="AC16G0_03830"
                     /inference="COORDINATES: protein motif:HMM:NF016959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HIR5098785.1"
                     /translation="MEFSPLEITQREFGRKLRGLDPEEVQTFLEQIAEEMTRLLQENT
                     DRAGQIQRLEGQLRVHQEREESLRNTLVTAQKMTEEIKANAKREADLLVRETELKAER
                     LLEDAHRKLGQVQAEIAELKRQRDLFASKLRGLLKTHLDLLEPEPEKPVSQA"
     gene            1811..2080
                     /locus_tag="AC16G0_03835"
     CDS             1811..2080
                     /locus_tag="AC16G0_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HIR5098786.1"
                     /translation="MAVQVHPRASRNQLVEQPPKCFTIRLTAPPVQGAANTACCAFLA
                     ELLGLAKSRVVLARGEASRQKLLRIRDADAGQVLARLQSLTVPQK"
BASE COUNT          373 a          734 c          676 g          365 t
ORIGIN      
        1 cccagtagag ggcaggacca tcccgcccgc gcccctgtat accccaggcg gagcacctcc
       61 cgcaagtcct cccgcgacga aacccgaggc cgtgaggctt gacttccgca gccgtgaacg
      121 atataaagcg ccgacggagg tgatgcatgc tccaaggcag acggctggga ttcgtgggcg
      181 gtggcaacat gggcgaggcc ttgctccgcg gcctcctggc ggccaagatc gtgggaccgg
      241 acagcctgct ggtctcggac gcgcgtgagg atcgcctcac cagtctgcgg caggcctacg
      301 gcgtccggac ccattccagc aacgccgagg tggctcgtca ggcggacatc ctgctgctcg
      361 cggtcaagcc ccaggtcata ggccaggccc tggatgatct ggtggaggcg ctggacgaga
      421 agaaactcgt catctccatc gctgcaggca tccccacctc cttcatcctg gaccgctttt
      481 cctttccgct gcgcgtcgtg cgggtcatgc cgaacacgcc cgcgctcgtc ctggagggca
      541 tgagcgccct gtcccttggc gtccacgcca cagccgagga tctggagacc acccggcttc
      601 ttttcgagac cgtggggaag gtcgtcgtcg tggacgagtc ccttctggac gccgtcaccg
      661 gtctttcggg gagcggcccg gcctatatct tcctcgtcat cgaggccctc gccgatgccg
      721 gcgtcaaggt gggcctcccc cgtgatgtgg cccacctcct ggcggcccaa acggttcgcg
      781 gcgcggcccg gatggccctg gaaacgggga agcatccagg tcagttgaag gatatggtca
      841 cctcacccgg cgggaccacc attgccggcc tgcatgccct ggagcagggg ggggtgcggg
      901 ccgcgttttt caacgcggtg gaggccgcca cccgccgctc tcgcgaactt ggacggcgat
      961 gatcgctgta cggcggatcc gcgcgccaca aggagaggag tgatgttcgt cgccggcaat
     1021 ttcctggatg ccgtcgcagc ggtcttgaat ttggcgctct gggcatacat gtggatcatc
     1081 atcgcccgtg ccctcatctc ctgggtgaac ccagacccct ggaaccccat cgtgcagttc
     1141 ctccaacggg ccaccgagcc ggtgctggcc cccatccgcc gtcgattgcc tagctggcag
     1201 tggggcatcg acttctcgcc gctggtggtc attctggtca tatacttctt gcaggcctgg
     1261 ttggtcggct cgctcagaga cctagcctgg aggatgcgct gatggaattc tccccactcg
     1321 agatcaccca gcgggagttc ggccggaaac ttcgcggtct ggatccggaa gaggtgcaga
     1381 cgttcctgga gcagatcgcc gaggagatga cgcggctcct ccaggagaac accgaccggg
     1441 ccggacagat ccagcggctg gaggggcaac tccgcgtcca ccaggagcgc gaggagtcgt
     1501 tgcgcaacac cctggtgacc gcccagaaga tgaccgagga aatcaaggcc aacgctaagc
     1561 gcgaggccga tctcctggtg cgagagaccg aattgaaggc ggagcgcctc ctggaggacg
     1621 cccaccggaa gctcggtcag gttcaagccg aaatcgccga attgaagcgc cagcgggatc
     1681 tcttcgccag caagctccgc ggcctgctca agacccatct ggacctgctg gagcccgagc
     1741 cggagaagcc ggtctcccag gcgtgaggac cgatcgtccg actgtcgtgc aggatggtcc
     1801 ggacgtgctc gtggcggtcc aggtgcatcc ccgcgcctcg cgcaatcaac tggtggagca
     1861 accaccaaaa tgcttcacca tccggctgac cgcgccaccg gtacagggcg cggccaacac
     1921 ggcctgctgt gccttcctgg ctgaactcct cggcctcgcc aagtcccgcg tcgtcctcgc
     1981 ccgcggcgag gcctcccgcc agaaactcct ccgcatcagg gacgccgacg ccggccaagt
     2041 tttggcccgc ctccagtccc tcaccgttcc ccaaaagtag aaggccatcc cccgttatcc
     2101 tcggagtacg cagacttcac agcccctccc cttcagacgg gaagaggc
//
LOCUS       DBNGZC010000210         2153 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1876628, whole genome shotgun sequence.
ACCESSION   DBNGZC010000210 DBNGZC010000000
VERSION     DBNGZC010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1876628"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1307)
                     /locus_tag="AC16G0_03840"
     CDS             complement(30..1307)
                     /locus_tag="AC16G0_03840"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR5098787.1"
                     /translation="MILPLVLAFVALLTVTVPVSVAMGLAAAIAILWGGKAPINTVVT
                     QMVSGMDSFPLMAIPFFILAGELMERAGISERLVHLAKAFVGHIRGGLGMVVLVGEYL
                     FSGISGSTAADISAIGSLLIAPMERAGYSKGQAVAIVSAATAMGILVPPCIFMIVLAS
                     MTNQSIGAMFVAGFLPAIVIAIGLGGYIYWEAGVLNIPREAKASRRERLRALGDGVIA
                     MVMPGIIFGAIFGGITTVTEAAVIAVLYSAFVGLAIYKRFTLKDLPKIILASTINTSM
                     CMLLIGAATVFSWVMTVERVPQIIAKLVMGVSTSPWFFLLLSNVIFVVFGAVLEGLPA
                     MIVFVPVMYPIAVQFGIDPLHYGILIIASIGIGVFLPPIGVALFIACAIAKLNIVESA
                     KFMAPYLVILFLGLLVVTYVPWFTLVLPHAFLK"
     gene            complement(1304..1789)
                     /locus_tag="AC16G0_03845"
     CDS             complement(1304..1789)
                     /locus_tag="AC16G0_03845"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR5098788.1"
                     /translation="MGQRVWGIYEGTVNWAAVALFGAMVVVTTLGVFFRYVLNNALPW
                     AEEADRYLFIWLSFVGASITMRHKAHIAVDLLVRYIRPNLRGYFALLAQLSVLAFLGV
                     VFWASLPVIELTSETRATATDIPMSWVYLAAPTGCILIGVETLRLMARTRAEMRGKEE
                     A"
     gene            complement(1859..>2153)
                     /locus_tag="AC16G0_03850"
     CDS             complement(1859..>2153)
                     /locus_tag="AC16G0_03850"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098789.1"
                     /translation="YTGHIFCPNPFLCSERWFSKLSKNVQQAIVEAGKESLEWQRKEA
                     QVSEKAFEQKLKDAGVTFTTPDPGPFRKAVEPFYKEWGDKYAGNDLIKQIREA"
BASE COUNT          434 a          679 c          707 g          333 t
ORIGIN      
        1 gctcgtgccg gccgcgggaa actccgcgcc tacttgagga aggcgtgcgg caggaccagg
       61 gtgaaccagg ggacgtaggt caccaccaag agtcccagga acaggatgac gaggtagggg
      121 gccatgaact tcgccgactc gacgatgttc agcttggcga tagcgcaggc gatgaacagc
      181 gccacaccga tggggggcag gaagactccg atcccgatgg acgcgataat caggatcccg
      241 tagtgcaggg gatcgatccc gaactggacg gcgatggggt acatcacggg aacgaacacg
      301 atcatggcgg gaagcccctc caggaccgcc ccgaagacga cgaagatgac gttgctcagc
      361 aggaggaaga accaggggga ggtcgagacg cccatgacca gcttggcgat gatctggggg
      421 acccgctcca cggtcatgac ccaggagaag accgtggccg cgccgatcag gagcatacac
      481 atggaggtgt tgatggtgct ggccaggatg atcttgggga ggtctttcag cgtgaagcgc
      541 ttgtagatgg ccagacccac gaaggccgag tagagcacgg cgatgaccgc cgcctcggtc
      601 acggtcgtga taccgccgaa gatggccccg aagatgatgc ccggcatgac catggcgatg
      661 acgccgtcgc ccagcgcccg caatcgctcg cggcggctag ccttggcttc gcgcgggatg
      721 ttgagtactc cggcctccca gtagatgtag ccccccagtc cgatcgcgat gacgatggcg
      781 ggcaggaatc cggccacgaa catggccccg atggactggt tggtcatgct ggccagcacg
      841 atcatgaata tgcagggagg caccaggatg cccatggccg tggccgcgct gacgatggcg
      901 accgcctggc ccttgctgta gcccgcccgc tccatggggg cgatgagcag cgagccgatg
      961 gcagagatgt cggcggcggt ggagcccgag atgccggaga agaggtactc gcccaccagc
     1021 acgaccatcc cgaggccacc ccggatgtgc cccacgaagg ccttggccag gtgtacgagc
     1081 cgctccgaga tcccggcccg ctccatcaac tctcccgcca agatgaagaa ggggatggcc
     1141 atgagcggga atgagtccat gccggagacc atctgggtca caaccgtatt gatgggcgcc
     1201 ttgccccccc acaggatggc aatggccgcg gccagaccca tggccacgga gaccgggacg
     1261 gtgacggtca atagggcgac gaaggcgaga accagcggga ggatcatgcc tcttccttcc
     1321 ctcgcatctc ggcccgggtg cgcgccatga gccgcagggt ttcgaccccg atcaggatgc
     1381 aacccgtcgg ggccgccagg tacacccagc tcatggggat gtccgtggca gtggcccgcg
     1441 tctcgctggt cagctcgatg accggcaggc tggcccagaa caccaccccc aggaatgcca
     1501 ggaccgagag ctgggccaag agggcgaaat atccgcggag attcggccgg atgtaccgta
     1561 ccaggaggtc cacggcgatg tgggccttgt ggcgcatggt gatggatgcc cccacgaagc
     1621 tgagccagat gaacagatac cggtcagcct cctcggccca cggcagggcg ttgttcagca
     1681 cgtaccggaa gaagacgccc agggtcgtca ccaccaccat cgcgccgaag agggccacgg
     1741 ccgcccagtt cacggtccct tcgtagatac cccagacccg ctgtcccatg cgtcccaccc
     1801 gatacaacga tgcgggcggc acagccgccc gcatcgcgtg taatgtttcc gccaggcctt
     1861 acgcctcgcg gatctgcttg atcagatcat tgccggcgta cttgtcgccc cactccttgt
     1921 agaacggttc cacggccttg cggaagggac cgggatccgg ggtggtgaag gtcaccccgg
     1981 cgtccttcag cttctgctcg aacgccttct cgctgacctg agcctccttc cgctgccact
     2041 ccagcgattc cttgccggct tccacaatgg cctgctgcac gttcttggac agcttgctga
     2101 accagcgctc gctgcacagg aagggattgg gacagaagat atggcccgtg tag
//
LOCUS       DBNGZC010000211         3214 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1191356, whole genome shotgun sequence.
ACCESSION   DBNGZC010000211 DBNGZC010000000
VERSION     DBNGZC010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1191356"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /gene="trpB"
                     /locus_tag="AC16G0_03855"
     CDS             <1..1062
                     /gene="trpB"
                     /locus_tag="AC16G0_03855"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR5098790.1"
                     /translation="YTLRQYVGRPTPLYLAGRLSDRLGGPRVYLKREDLCHTGAHKIN
                     NTVGQILLARRLGKRRVIAETGAGQHGVATATVAALLGLSCEVYMGTEDMRRQALNVV
                     RMRLLGATVTPVDAGSRTLKDAINEAMRDWVTNVDTTHYVLGSVLGADPYPRMVRDFQ
                     TVIGREARRQILEAEGRLPDCLIACVGGGSNSIGLFHEFLGDPAVRMIGVEAGGKGIA
                     SGQHAARFAERTVGILHGTKSYVLQDANGQIRVTHSISAGLDYSGVGPEHGYYHDQGR
                     IEYTSITDAEALEGFRLLCETEGIIPALESAHAVAYLTRIAPELGAKSCVILNLSGRG
                     DKDVEVVAEALGLKTGGVA"
     gene            1153..1953
                     /gene="trpA"
                     /locus_tag="AC16G0_03860"
     CDS             1153..1953
                     /gene="trpA"
                     /locus_tag="AC16G0_03860"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIR5098791.1"
                     /translation="MTENRIDRAFARLRETERKGLIPFLAAGDPSLEITEALVREFAE
                     RGADVVEIGVPFSDPLADGVVNQRAYQRALVNGAGLGQILELVARIRRHSEIPLVLMS
                     YVNPIHRFGLERFPAAARDAGVDGLILSDCPPEEMDGFLEAMGQAGVHPVLLVAPTSP
                     DRRISLISARGRGYIYYVSLRGVTGQRDHLPDDLAAGIRRVRALTEKPLAVGFGISTP
                     GQAASTAALADAVIVGSAIVAKIESSLGSRGLVARVGEFVKDLRSAVG"
     gene            2337..2771
                     /locus_tag="AC16G0_03865"
     CDS             2337..2771
                     /locus_tag="AC16G0_03865"
                     /inference="COORDINATES: protein motif:HMM:NF023444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly FimT family protein"
                     /protein_id="HIR5098792.1"
                     /translation="MTAVELITVMVLLVILAAFAIPALSPVVLRQRLTGAAWQLGGDL
                     RLARQRAVTERKRFRVCLTSCAISVPSGGYSIEREDGGWVSETGVPNRLPPDVTVTTT
                     GTATFATNGMASGGTFTVSSLIGSYQITVAATGRVKVCEGTC"
     gene            2768..>3214
                     /locus_tag="AC16G0_03870"
     CDS             2768..>3214
                     /locus_tag="AC16G0_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098793.1"
                     /translation="MRLRREAGPAGLTLIEFLIATSIMAFAALGVAGMFPAAFRSVVV
                     GGQVTKATALAQEMVDMIRNEPFDLLVSHYDGFDTRTLSITCPVTGVDLNDNKKKWTC
                     DLLGAGAQISGRGIPGGYGIVTVTDSMADLRQVTVTVFWDRDGSRSV"
BASE COUNT          564 a          929 c         1128 g          593 t
ORIGIN      
        1 tacacccttc gccaatacgt gggccgcccc acccccctgt atcttgcagg ccggctgagc
       61 gaccggctgg gcgggccacg ggtgtatctg aagcgggagg acctgtgcca caccggagcc
      121 cacaagatca acaacacggt ggggcagatc ctgctagccc ggcgattggg gaagcggcgg
      181 gtcatcgccg agacaggggc ggggcagcac ggggtggcca cggccacggt tgcggccctt
      241 ctcgggctgt cctgcgaagt ctatatgggc actgaggaca tgcgacggca ggccttgaat
      301 gtggtgcgca tgcggctcct gggggcgacc gtcacccccg tggatgcggg aagccggacg
      361 ctgaaggacg ccatcaacga ggctatgcgg gattgggtga cgaacgtgga cacgacccac
      421 tacgtcctgg gatcggtgct gggcgcggat ccctaccccc gtatggtccg agacttccag
      481 acggtcatcg gtcgagaggc gcgccgccaa atcctggaag cggaggggcg gttgccggat
      541 tgcctcattg cctgcgtcgg cggcggaagc aattccatcg gtctctttca cgaattcctg
      601 ggggatcccg cggtgcggat gatcggcgtg gaggccgggg gcaaggggat cgcctcgggg
      661 cagcatgccg cccggtttgc cgagcggacg gtgggtatcc tgcatgggac gaagagctac
      721 gtcctgcagg atgccaatgg gcagattcgc gtcacccact ccatctcggc gggcctggat
      781 tactccggtg tgggcccgga gcacggctac tatcacgacc aagggcggat tgagtacacc
      841 tcgatcacgg acgcggaagc tctggaggga ttccggttgc tctgcgagac cgaggggatc
      901 atcccggcgc tggaatccgc gcacgcggtg gcatacctca cgcggattgc gccggaactc
      961 ggggccaagt cgtgcgtgat cctcaacctg tcgggtcggg gggacaagga cgtggaggtt
     1021 gtggcggagg ccctcggcct gaagacgggt ggtgtggcgt gactcatcga gagaccagag
     1081 atcagagacc agagatcagc gatcagaggt cagaggccag agaagcagtc ggcctgaaga
     1141 cggaggtggg gtgtgacgga gaaccggatc gatcgtgcgt tcgcacggct gcgagaaacg
     1201 gaacgcaagg gactgatccc cttcctggcc gccggtgacc catccctgga gatcacggag
     1261 gccttggttc gggagttcgc ggagcgcggg gcggacgtgg tggagatcgg cgtacccttc
     1321 tcggaccccc tggcggatgg cgtggtgaac cagcgggctt accagcgggc cctggtaaat
     1381 ggagccggcc tgggacagat cctcgaactt gtggcgcgaa tccggcggca ttcagagatt
     1441 cccttggtcc tcatgagcta cgtcaacccc atccaccgtt ttgggctgga gcgctttccg
     1501 gcggcagcac gggacgccgg ggtggatggt ctgatcctct ccgactgtcc acctgaggag
     1561 atggacggat tcctggaagc tatgggccaa gcgggggtcc acccggtctt gcttgtcgcc
     1621 ccgactagtc cagaccggag aatttccttg atcagtgccc gaggccgtgg ctatatatac
     1681 tatgtctcct tacgtggggt gaccggccag cgggatcatt tgccggacga cttggcagcc
     1741 ggcatccggc gggtgcgggc cctcacggag aagcccctgg cggttgggtt cgggatctcg
     1801 acccccggtc aagcggcttc cacagccgcc ctggccgatg ccgtgatcgt cggatcggcc
     1861 attgtggcaa agatcgaatc ctcgttgggt agccgaggtc tggtcgcccg tgtaggggag
     1921 tttgtgaagg acctccgttc tgccgtgggg tgaccgggcc ctgcccccga acgcgaggga
     1981 ggaggttcgc gtggccctcg atttgccgaa tctcttcccg ttacctaact gggccgccca
     2041 cctttgcgtg gttcccgtct gggccacgtc ttccactctt tccagcagaa gtacaccgat
     2101 cgaggcctgg ctttcaggcc gcgacggaag agatactgtg tgccaggagc gaccgtgacc
     2161 tcgcccgaca tgagaggtga cccgaagccc tgactgctcg gaggtggcca ggtgggggcc
     2221 gggcggagcg ccgatggctc attggctcat gatccgtgac tgcggaatac cgctcgcggc
     2281 cgagggctat atacgtgttc gcgaagaggt ttgagctgcg acgaaaggcg cgcggcatga
     2341 ctgcggtgga gttgatcacc gttatggtgc tcttggtcat cctcgcagcc ttcgctatcc
     2401 ccgccttgag tccggtggtc ctgcgacaac gattgactgg cgcggcatgg caactgggcg
     2461 gggatcttcg attggcaagg caaagggcag ttacggagcg gaagcggttc cgggtctgcc
     2521 tcacgagctg tgcgatcagc gtgccgtcgg gcggctacag catcgagcgc gaagacgggg
     2581 ggtgggtcag cgagaccggt gtgccgaaca ggctcccccc cgacgtcacg gtcaccacca
     2641 cggggaccgc aacctttgcg acgaacggga tggccagtgg gggtaccttc accgtatcca
     2701 gcctcattgg gtcctaccag atcacagtcg cggccaccgg ccgcgtcaag gtatgtgagg
     2761 gaacgtgttg aggctcaggc gagaagctgg tccggccggg ttgacgctga tcgagttcct
     2821 gatcgccacg tcgatcatgg cttttgcggc cctgggcgtt gccgggatgt ttccagcggc
     2881 atttcgctct gtcgtcgtcg gtggccaggt gactaaggcg acagccttgg cacaggaaat
     2941 ggtcgacatg atccggaatg agcccttcga tcttctggta tcgcactatg acggatttga
     3001 cacgcgaacc ctaagtatca cgtgtccagt caccggtgtg gacttgaatg acaacaagaa
     3061 gaaatggacg tgcgatctcc taggagcggg cgcccagata tcagggcggg gtatccccgg
     3121 cgggtatggg atcgtgactg tgaccgactc catggcggac ctgcgtcagg tgaccgtgac
     3181 ggtgttctgg gaccgcgatg gctcacgctc ggta
//
LOCUS       DBNGZC010000212         2313 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2071562, whole genome shotgun sequence.
ACCESSION   DBNGZC010000212 DBNGZC010000000
VERSION     DBNGZC010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2071562"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(464..835)
                     /locus_tag="AC16G0_03875"
     CDS             complement(464..835)
                     /locus_tag="AC16G0_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098794.1"
                     /translation="MRPDSRPAHIPRSTPALIAELRMHAAGLTLAAIAVVHNDSTRFV
                     FPTDDDPLSRLNTLIHAGGHPIGIVGAQIGHGAIRFVLHPFVEFQNEPQAIEYLHTLR
                     DPFLTLIRTHVDRMPDNPRNN"
     gene            1208..>2313
                     /locus_tag="AC16G0_03880"
     CDS             1208..>2313
                     /locus_tag="AC16G0_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR5098795.1"
                     /translation="MTRAEDDPRAELEERLRFEALLADLSARFVSVPPDQMDGLIEEA
                     QRRIVQALGLDRSTLLQRADAEDDLVITHAWAEPGFTPQPGLFAQRDFPWVHQTLLRG
                     GTVRFSKIEELPAAAARDKASFGHTQQKSNLIFPLASTGRVFGALSFGTLSAEREWPD
                     GLVQRLQLMADIFANALARQRGEQALRRALDDVTRLKQQLEAENAYLREAAQVQPSDI
                     VGHSPALKQVVSQADHVAPTDTAVLLLGETGTGKGLLAQLIHRRSKRQDRAMVTVNCA
                     ALPPTLIEAELFGREKGAYTGALTRQAGRFELAHGSTIFLDEIAELPLELQVKLLRVL
                     EDGSLERIGSTKTLRVDVRVIAATNRDLAKRVAQG"
BASE COUNT          413 a          719 c          801 g          380 t
ORIGIN      
        1 ttgcagcttt ggtgggtagc gcttacgagc atacccagcg taggcgcagc tcggcagcct
       61 cccatgtcag tatggctgac ctgcagccta caggcccaag ccgcgcgccc gccggtgggg
      121 cgcggcgaga ccggcgggcg gggcgagtgg agggagagtg tcaccgatgt tgattattag
      181 gacaaaaccg tcgagagagg cggtctcggc agcggggagg ccacccgtct cccagcagga
      241 tgctgaataa cccttcttca gctaggctga tcaagagatc cagatgtagg gcggcggacc
      301 catctttgga tggggaccgc cgcgaggtac ccaccggaac tgggtcggag gcgtacccag
      361 tctgtacgtc gcagcgttgc gcggcgcacc aagtacacat ccacggatgg gtacccatcc
      421 aatgggatgg gtccccggca gatgggcctt ctccagcagc ctgctagttg ttccgagggt
      481 tatccggcat gcggtcgacg tgcgtgcgga tgagggtcag gaagggatcg cggagcgtgt
      541 gcagatactc gattgcctgc ggttcattct ggaactcgac aaaggggtgg aggacaaatc
      601 tgatggcgcc gtggccaatc tgggcgccga cgatgccgat gggatggccg cctgcatgaa
      661 tgagggtgtt gagcctgctc agcgggtcgt catctgtagg gaagacgaac cgggtagagt
      721 cgttgtgtac aaccgcgatc gcggccaagg tgaggccggc agcatgcatt cgcaactcgg
      781 cgatgagtgc gggggttgac cgggggatgt gtgcgggccg actgtcagga cgcatcgttc
      841 tcctcggcga cacgtatcgc cgactgggaa gtcgccttaa ggtaacatgc caaggcggga
      901 tctcctacga acggatcacg cggttgtacg gtggcccgcc ccgatgaata ccagccagaa
      961 gaaggtaacg gcgtgaggct ggcgattctc tgtgcaggcc acggctcgtg gacgccgcag
     1021 ccaaattctg gtagcatcca acaaataaaa cgtcgaagtt gatggtccac gcccgccttg
     1081 acccggcatc cccagagcgg gccgaaagga gggttctcgc tttgcgtacg atcagtcccg
     1141 catggcatct atcgacgctt gaatgggtga ccgctcttga gaccggtgcc atgggagaca
     1201 cgccatcatg acccgtgcgg aagatgaccc gcgggcggaa ttggaggagc ggctgcgctt
     1261 cgaggcactc ctcgccgatc tctcggcccg cttcgtcagc gtgccgcctg accagatgga
     1321 cggcctgatc gaggaggccc agcggcgcat cgtccaggcg ctggggctgg accgcagcac
     1381 cctcttgcag cgagcggacg cggaggacga cctcgtcatc acgcacgcct gggccgagcc
     1441 gggcttcacc ccgcagcccg gactctttgc ccaacgggac ttcccctggg tgcatcagac
     1501 gctactccgg ggcgggaccg tccgtttctc gaagatcgag gaactgcccg cggcagccgc
     1561 gcgcgacaag gcctccttcg gccacaccca gcagaagtcc aatctgatct ttccgctggc
     1621 gtccactggt cgggtcttcg gggcgctctc cttcggcacc ctgtcggcag agcgcgagtg
     1681 gcccgacggg ctggtgcagc gcctgcagct catggcggac atcttcgcca atgccctggc
     1741 gcgccagcgg ggcgagcagg ccctccggcg cgccctggac gacgtcacgc gcctgaagca
     1801 gcaactcgaa gccgagaacg cctatctgcg cgaggccgcc caggtccagc ccagcgatat
     1861 cgtcggccac tcgccggcgc tgaagcaggt ggtgtcccag gcggaccacg tcgcgccgac
     1921 cgacaccgcc gtgctcttgc tgggggagac tggcaccggc aaagggctgc tcgcgcagct
     1981 catccatcgc cggagcaagc gccaggaccg ggcgatggtc acggtgaatt gcgcggcgct
     2041 ccccccgacc ttgatcgagg cggagctgtt cggccgggag aagggggcct acaccggggc
     2101 cctgacccgc caggccgggc ggttcgagct ggcgcatggc tcgaccatct tcctcgacga
     2161 gatcgccgag ctgccgctgg agctgcaggt caagctgttg cgggttctgg aggatgggtc
     2221 gctggagcgg atcggcagca ccaagaccct cagggtggac gtccgggtga tcgccgcgac
     2281 caaccgggat ctcgccaagc gggtggccca ggg
//
LOCUS       DBNGZC010000213         1745 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1973464, whole genome shotgun sequence.
ACCESSION   DBNGZC010000213 DBNGZC010000000
VERSION     DBNGZC010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1973464"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1719)
                     /locus_tag="AC16G0_03885"
     CDS             complement(<1..1719)
                     /locus_tag="AC16G0_03885"
                     /inference="COORDINATES: protein motif:HMM:NF015237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR5098796.1"
                     /translation="MGAVVRYVRLRRGLQIVELPRLDLNDAQHRTVLATTFVGAIALL
                     GFSAIGGYQAYHFSDSVEFCGQACHTVMQPEFTAYQNSPHARVPCVECHIGPGAEWFV
                     RAKISGAYQVYSVLFNKYDRPIPTPISNLRPAQETCEQCHWPAKFWGKQLATRVHFSS
                     DKTNTRREIQLLVKTGGGSVKGLSEGIHWHMNIANKTWYLATDERRQVIPWVRVQDPD
                     GRITEFVSTEHRISPEQLKQGEVRRMDCMDCHNRPSHKYLPPGRALDVSLLVGRISTD
                     IPFIKKVAVEAMVKPYATTPEAERGIETYIREFYSKEYPQVGKAREDRLRNAIAEVMQ
                     VYRQNFFPEMKVNWQAYPDNIGHKEFPGCFRCHDGKHVSADGRILRRDCGTCHDFLEA
                     KKSGDLALVAATAAFAHPWKLGGRHAEIQCGTCHDGGPAKPATCRECHKLPASGAPMT
                     GMECKQCHLKEQQRQPLADCKTCHGSPAGLHQAAMHSAAGCTACHVPHGWSPEPRKQC
                     LTCHADKAEHNPGLPCAQCHAFRAAAPGAQTSAVSAPPAITFPKDPSSLGPVTFIHAA
                     HLARGAK"
BASE COUNT          296 a          542 c          569 g          338 t
ORIGIN      
        1 tttggcaccg cgagccagat gggccgcatg tatgaaggtg acgggcccca gcgagctggg
       61 atccttgggg aaggtgatcg caggcggcgc ggagaccgcc gaggtctggg cgccgggggc
      121 agcggcccgg aaggcgtgac actgggcgca gggcaggccg ggattgtgtt ccgccttgtc
      181 cgcgtggcag gtcaagcatt gcttccgcgg ctccggcgac cacccatggg ggacgtgaca
      241 ggcggtgcag ccagcggcag aatgcatggc tgcctggtgc aggccagccg gggaaccgtg
      301 acaggtcttg cagtccgcca gcggctgacg ctgttgttcc ttcaggtggc attgcttgca
      361 ttccatcccc gtcatgggtg cgcccgacgc ggggagcttg tgacattccc ggcaggtggc
      421 gggcttggct ggacccccat cgtggcaggt tccacactga atctccgcgt gcctcccccc
      481 gagcttccac ggatgggcga aggccgcggt cgctgccacc agcgccagat ctccgctctt
      541 cttcgcctcc aggaagtcgt ggcaggtccc gcaatcgcgg cgaaggatgc ggccatccgc
      601 gctgacgtgt ttcccatcgt gacagcggaa gcaacccgga aactccttgt gcccgatgtt
      661 gtccggatag gcctgccagt tgactttcat ctcgggaaag aagttctgcc ggtagacctg
      721 catgacctcg gcgatggcat tccgcagacg atcctcccgg gccttcccta cctgcggata
      781 ttctttcgag taaaactctc gaatgtacgt ctcgatccct cgctcggcct ctggcgtggt
      841 ggcgtagggc ttgaccatgg cttccaccgc caccttcttg atgaatggaa tatcggtgga
      901 aattcggccc accagcagag acacgtccag cgctcgaccg ggtgggaggt atttgtggct
      961 gggccgattg tggcagtcca tacaatccat ccggcgcact tcgccttgct tcagttgctc
     1021 cggcgagatg cggtgctctg tggagacgaa ctccgtgatc cgcccatccg gatcctgaac
     1081 gcggacccag ggaatcacct gtcgtcgctc gtcggtggct aggtaccagg tcttgttggc
     1141 gatgttcatg tgccagtgaa tcccctcgct cagtcctttc accgaaccac caccggtctt
     1201 caccaggagc tggatctccc ggcgcgtgtt ggtcttgtcc gagctgaaat ggacccgggt
     1261 cgccagctgc tttccccaga acttggccgg ccagtggcac tgctcgcacg tctcctgcgc
     1321 ggggcgcagg ttgctgatgg gcgtggggat gggtcggtcg tacttgttga agagcaccga
     1381 gtacacctgg tacgccccgg agatcttggc ccgcacgaac cactccgccc ccgggccgat
     1441 atggcattcg acgcacggga cccgggcatg gggggaattc tgataggcgg tgaattcggg
     1501 ctgcatcacc gtatggcaag cctgcccgca gaactccacg gagtcgctga aatggtaggc
     1561 ttgatagcca ccgatggcgg aaaatccgag cagggcgatg gccccgacga aggtcgtggc
     1621 caggaccgtt cgatgttggg catcgttgag gtccagccgg ggcagctcaa cgatctgcag
     1681 gccgcgacgc agccggacat atcgaacgac ggcgcccatg agcaccaacc ccattccgct
     1741 cagga
//
LOCUS       DBNGZC010000214         1853 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1289658, whole genome shotgun sequence.
ACCESSION   DBNGZC010000214 DBNGZC010000000
VERSION     DBNGZC010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1289658"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            513..>1853
                     /locus_tag="AC16G0_03890"
     CDS             513..>1853
                     /locus_tag="AC16G0_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098797.1"
                     /translation="MYEALRAYFVEERSSAEVAKAFGYTPGSFRVLCHQFRRDDDPTF
                     FVKALRGRRSQPKKSAARDLIVELRKQNHSVYEISEELKSRHLLLSPTAVREVLKEEG
                     FAPLPRRLDEERPYRLGPTAEPIADVRSFSLAPRSVSTSCGGLVLFVPDLIRLELDRL
                     ARAAHLPGSRMIPAEHALRACLALKLWCVERRSHVMALVADEGLALFAGLNAIPKKSF
                     LSEYSSRVDPAKTSRLLATWHQLVEGQHLFAGDSFNLDFHSVPYYGDDPVLERHYVSA
                     RSRRQPSVLAFLAQDAEGRAFCYANADIRKGEEAEEIFRFIAFWKRTHGQNPRHLVFD
                     SRLTTYPGLVRLEKMGIVFITLRRRTAKLLQEVAALPRSAWHTIELDVPTRKYKTPRI
                     YEQTVALAGGSFRQIFIRDLGHDEPTLLLTNDRRTSAPKLITRYAQRMLIENALS"
BASE COUNT          336 a          659 c          527 g          331 t
ORIGIN      
        1 cgacgagctc caccgggtct ggatcctgcg caaggtcctc tcgccgctct cccaggtcga
       61 ggcgatggag ctcatcctcg acaagctcgg gaagacgaag ggcaacaagg acttcctgga
      121 caacatggcg gtgggctagc ctggatttcc acggcgcggc attgagctaa ccaagtcagc
      181 cacagtctac gttgctgagc tcgttccgct ggtgccagag gaggtcggcc tggccactaa
      241 cagcggcgag tgttagccca agggtgtgct gatcctggtg cagcgttggg ccgccccacg
      301 agatacgcga cgggcggggt caaggggacg gctgcggaga gccgaggtgg cgcgctgtgg
      361 gcgcggtcgt cgaagcccca tgggtagggg tgaagattcc ccttgacccc aggtctagat
      421 agtagtaccg tgcagtcgtg ccattgggag caccaaaaga cataacggag tgtagacgct
      481 tcttcctcga gccggatggc ccgaagcacc ggatgtacga ggccctcagg gcctatttcg
      541 tggaggagcg ctcctccgcc gaagtcgcca aagccttcgg ctatacccca ggatccttcc
      601 gtgttctgtg ccaccagttc cgtcgtgacg acgaccccac cttcttcgtc aaggcgctgc
      661 gcggccgtcg ctcacagccc aagaagtcgg ccgcgcgcga cctgatcgta gaactgcgca
      721 aacaaaacca ctcggtctac gagatcagcg aggaactcaa gagccgccac ctgctcctga
      781 gccccaccgc cgtgcgcgag gttctgaagg aggaggggtt cgctccgttg ccccgacgcc
      841 tcgacgaaga gaggccctac cgattgggcc ccaccgccga gcccatcgcc gacgtgcgct
      901 cgttttcact cgctccccgc agcgtgagca ccagctgcgg cgggttggtc ctcttcgtac
      961 ccgacttgat tcgcttagag ctggacaggc tggcgcgcgc cgcccacctg cctggctcca
     1021 ggatgatccc cgccgagcat gccctgcgcg cctgcctggc tctcaagctg tggtgcgtcg
     1081 aacgccgcag ccacgtgatg gccctggtcg ccgacgaagg cttggccttg ttcgcaggac
     1141 tcaatgccat ccccaagaag agtttcctct ccgagtactc ctcgcgggtt gatcccgcca
     1201 agacttcccg cctgctcgcc acctggcacc agctcgtgga aggccagcac ctcttcgccg
     1261 gcgattcctt caacctcgat ttccactccg tcccctacta cggcgatgac cccgtcctcg
     1321 agcgtcatta cgtctccgcc cgctctcgtc gccagcccag cgtcctggcc ttcctcgccc
     1381 aggacgccga gggccgcgcg ttctgctacg ccaacgccga catccgcaaa ggtgaggagg
     1441 ccgaagagat cttccgcttc atcgccttct ggaagcgaac ccacggtcag aacccgcgac
     1501 atctggtctt cgactcccgg ctgaccacct atcccggctt ggttcgcctg gagaagatgg
     1561 gaatcgtctt catcaccctg cgtcgacgca cggccaagct cctccaggag gtggccgcac
     1621 tgccacgttc cgcttggcac accatcgaac tcgacgtccc cacccgcaag tacaagaccc
     1681 cccgtatcta cgaacagacc gtcgccctcg ccggaggaag cttccgacag atcttcatcc
     1741 gcgacctcgg tcacgacgag cccacgcttc ttctcaccaa cgaccgtcgc acctcggccc
     1801 ccaagctcat cactcgatac gcccagagaa tgctcatcga gaacgccctc tcc
//
LOCUS       DBNGZC010000215         7374 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1191567, whole genome shotgun sequence.
ACCESSION   DBNGZC010000215 DBNGZC010000000
VERSION     DBNGZC010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7374)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7374)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7374
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1191567"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="AC16G0_03895"
     CDS             <1..1142
                     /locus_tag="AC16G0_03895"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098798.1"
                     /translation="DLEAKLTAALAAGAPPEASHGQAFTCASFYHKGLLRDQEDIANA
                     VGKDNIFEVARNLCRFDGKYYGVTHSPNTNLLVYRKDIFKQKGLKPPETWDDFIKVAQ
                     AMMERDATGRVTRYGLSLPGVPLFVNILVAELTKANGGRLFDGKTGRPTFTEKQVVEV
                     LDFYKKMNDTSLPPGWLGHGYLDTFTNLATGKVATVYQGHGRSTGYIEKYAPPGTGTP
                     EYFGVMRKPHGPSGKETAAQVDAEPWMIFKNSKYPEEAAEFIKFFFQEKNYVKYLHTV
                     PVHLLPTLKSTRNDPAYQANETYRRWKEWVDMDYYYFERDLAKPTLVVNWDDLKLPFL
                     LEIFGSNILPDMVTDAVKGMPSAQAAAKAQTRAEELITKLGYKRW"
     gene            1251..2153
                     /locus_tag="AC16G0_03900"
     CDS             1251..2153
                     /locus_tag="AC16G0_03900"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5098799.1"
                     /translation="MTQQPSQGQPWRHQLTGFLVVFPALALILGLIVYPVAYSIWLSL
                     LEKHSFFPQQKFIGLENYVYLWSDEEFWTSLWLGVVYSFWTISFQLILGVAAALILNE
                     SFLGRGLVRGIVLFPYMIPTIVAVILWKWLLNDTYGVVNYWLFALGIVRDPISWLGTD
                     HIMFSTIVMSVWQFFPFVLLSILARLQTIPQELYEAAKVDGASAVRRFTHVTLPQIRG
                     ILFVVILLRSIWMFTKFDTVWLMGEGAGAGRYIRTLPVYAYMRTLTYYQAGLGAAIAV
                     IMFAILVVTTVIYFGLFREEADIG"
     gene            2150..2992
                     /locus_tag="AC16G0_03905"
     CDS             2150..2992
                     /locus_tag="AC16G0_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5098800.1"
                     /translation="MNRWRVRYLSSRGLLYAGAVFLTLQAAFPFFWMVSTSFKPPREV
                     FAQPPTFIPQGPTWDNFSRLFTTTNFLIYFWNSAYVCGLTVLLTMLVSAAGAYSLTRY
                     RYPGREQIAGLILCTYMFAPIMVVIPFFILVKKIGIENTHFALVLALTSFCLPFTLWL
                     LRAFFQTIPIELEQAAMVDGAGRLRAVIYVVLPLALPGIIATSIFTFILAWNDYIFTR
                     ILITSDELKTLPVGVQDLFHSALVDWGLIMGAGMMITIPALLFFVTVQRYLIRGWGAG
                     AVKG"
     gene            3017..4036
                     /locus_tag="AC16G0_03910"
     CDS             3017..4036
                     /locus_tag="AC16G0_03910"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5098801.1"
                     /translation="MAAFRFVLLGTGFYARKWLELLKSRADCEVVGIASRLAARAEEL
                     KRDFELPGAAVYPGWEAGSAAGSADGVLIALPQLFHPPAIIRALQAGLHVLVEKPLSL
                     DMASARAVYEEAGRHPGLAVMVNQNYRWRPHIRALRGAIREGLVGRVGHIQIEVRQQI
                     RRTTVGGWREQMAEPFLLDFAIHHFDLIRHLTGDEATRVLGTSFRPSWSWFDGNSAAA
                     AILSMRSGCVVDFGGTMVSQGHETTQEGVITVIGEKGTLQLDGESQVSLHGQGDARAL
                     PQDPVPGGELGYAMTEFMGAIRENRQPETNVAEHLRSLALPLAVVESARRKAPVEMAD
                     LLSFL"
     gene            4106..5221
                     /locus_tag="AC16G0_03915"
     CDS             4106..5221
                     /locus_tag="AC16G0_03915"
                     /inference="COORDINATES: protein motif:HMM:NF042587.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HIR5098802.1"
                     /translation="MSAQNLSVLEALTRHPLLLLPNRVYRVWTGGALLDRLQGKPDPE
                     DSHFPEEWVGSTTMTGLPGRPAEEGLSRVRLPDGSTQLLKMLIEAHPDAMLGAAHVAR
                     YGAELGVLCKLLDAGMRLSIQAHPNRDFARAHLHSEFGKTESWLVIGTRVINGEPPCI
                     LFGFRDGVTEEEFRRITRAQDTQKQVEALNRLAVRQGEVYLVPAGTPHAIGSGVFVVE
                     VQEPTDYVVNAEYTLGAIRRTEAQCYMGLGFDLGMQCFDYQAVGMDFVRQHTLVPRIL
                     HEDDQGREEILIGPEDTPCFGAVRLGVRGAVPDRDRGRCYVGIVIEGQGRLLGTDIAM
                     PLRAGVTFFVPAASRHEGYQAAADFPLTLIKCFPPPP"
     gene            complement(5245..5658)
                     /locus_tag="AC16G0_03920"
     CDS             complement(5245..5658)
                     /locus_tag="AC16G0_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098803.1"
                     /translation="MKGALLLLLAFLLGTATGALGFALYRSHAWWWGPPQDATRFREV
                     VIRRLTCELDLRPEQQQQVEAILREAGDQFTRLREEMRPRFLEIRNRTRERIQTVLDA
                     DQQARFRAYVDRLDRERERHRGPAQAPVATPARSP"
     gene            complement(5667..6125)
                     /locus_tag="AC16G0_03925"
     CDS             complement(5667..6125)
                     /locus_tag="AC16G0_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR5098804.1"
                     /translation="MGSCGELKRRLEELATVRAGELPETVRLHLAGCPACSALLARER
                     LVRGLVAAATEGVEPPVGFAEKVLASLPRNPAPAPVEMDLWRPAWGLVPAFAATAAVF
                     LFLFFQGSPAPSPSGFLPTENLSTSERLVLESPAPDLDLILTAVFGQGEL"
     gene            complement(6136..6744)
                     /locus_tag="AC16G0_03930"
     CDS             complement(6136..6744)
                     /locus_tag="AC16G0_03930"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5098805.1"
                     /translation="MAEPDGRQKGAEDPDEATLVQRAREVDREAFGALVRRYQRLVFR
                     VVGGFLRNPADVEDVAQEAFVKAFTAMSGFHPGAPFAPWMIRIATRASYDRLRQRRRA
                     SETAWEDLAPGEQRAAQDLMRGAQPEDQTAARDLAERALACLAPKDRQALILTHLQGY
                     TTAEVAETMGCSALAVRLRLHRGRRAMRQTVEGLLNRMKGSE"
BASE COUNT         1278 a         2436 c         2335 g         1325 t
ORIGIN      
        1 gcgacctgga ggccaagctg acggcggccc tagccgcggg cgcgcccccg gaggccagcc
       61 acggccaggc attcacctgc gcctcgttct accacaaggg actcctgcgt gaccaggagg
      121 acatcgcgaa cgcggtcggg aaggacaata tcttcgaggt ggctcggaac ctgtgccggt
      181 tcgacgggaa gtactacggc gtcacccact ctcccaacac gaacctcctc gtctaccgga
      241 aggacatctt caagcagaag gggctgaagc cgccggagac ctgggacgac ttcatcaagg
      301 tcgcccaggc catgatggag cgggatgcca cgggccgggt gacgcgctac ggcctctcgc
      361 tgcccggcgt tccgctcttc gtcaacatcc tggtcgccga gctgaccaag gccaacggcg
      421 ggcggctctt cgatgggaag accggccggc ccaccttcac cgagaagcag gtcgtggagg
      481 tgctcgactt ctacaagaag atgaatgaca cgagtctccc tccgggatgg ctggggcacg
      541 gctatctgga caccttcacc aacctggcca cggggaaggt cgccacggtc taccagggcc
      601 acggccggag caccggctac atcgagaagt acgccccacc ggggacgggc acgcccgagt
      661 acttcggggt gatgcgaaag ccccacggcc cgtccgggaa ggagacggcc gcccaggttg
      721 acgccgagcc ctggatgatc ttcaagaact ccaaataccc ggaggaggcg gccgagttca
      781 tcaagttctt cttccaggag aagaactatg tcaagtactt gcacacggtc cccgtccatc
      841 tcctcccaac gctcaagtca actcggaatg atccggccta ccaagccaac gagacgtacc
      901 ggcgctggaa ggaatgggtg gacatggact actactattt cgagcgcgat ctggccaagc
      961 cgacgctggt cgtgaactgg gacgatctga agctgccgtt cctgctcgag atcttcggct
     1021 ccaatatcct tcccgacatg gtgaccgatg ccgtcaaggg gatgccctcg gcccaggcgg
     1081 cggccaaggc ccagacccgg gcggaggaac tgatcaccaa gctcgggtac aagcgctggt
     1141 agctcgtggc ctagaagcgg ctcgtaactg tacggcttcc atccagcaac ctccaccccc
     1201 cggggggcgc cccgattcgg gacgccctcc gggggcttcc agtggtgaca atgactcaac
     1261 agccttcgca aggacaaccg tggcgccacc agctcaccgg cttcctcgtc gtcttccccg
     1321 ctctggctct gatcctcggc ctcatcgttt atcctgtcgc ctattcgatc tggctgagcc
     1381 tgctggagaa gcattcgttc ttcccgcagc aaaagttcat cggtctggag aactacgtct
     1441 acctgtggag cgatgaggaa ttctggacca gcctctggct gggggtggtc tactccttct
     1501 ggacgattag cttccaattg atcctgggag tggcggcggc cctcattctg aacgagtcct
     1561 tcctggggcg cggcctggtg cgagggatcg tcctctttcc ctacatgatc ccgaccatcg
     1621 tagcggtcat tctctggaag tggctgctga acgacaccta tggggtcgtg aactattggc
     1681 tattcgccct ggggatcgtg cgggacccga tcagttggct gggcaccgat cacatcatgt
     1741 tctccaccat cgtgatgagc gtctggcagt tcttcccctt cgtcctgctc tccatcctgg
     1801 cccggctgca gaccatcccc caggagctgt acgaggccgc caaagtggac ggggcctcgg
     1861 ccgtccggcg cttcacccac gtcacccttc cgcagatccg gggcatcctg ttcgtggtga
     1921 tcctcttacg cagcatctgg atgttcacca agttcgacac cgtctggctc atgggggagg
     1981 gtgcgggggc cgggcggtac atccggacgc tgccggtcta cgcctacatg cggaccctca
     2041 cctattatca ggcgggcctg ggcgcggcca tcgcggtgat catgtttgcc atcctggtgg
     2101 tcaccaccgt catttacttc ggtctgttcc gcgaggaggc ggacatcgga tgaatcgctg
     2161 gcgtgtgcgg tatctctcgt cccgcggtct cctgtacgcg ggggcagtct tcctcacgct
     2221 gcaggccgcg ttccccttct tctggatggt gtccacctcg ttcaagcctc cacgggaggt
     2281 cttcgcccag ccgcccacgt tcatccccca gggtcccacc tgggacaact tctcccggtt
     2341 gttcaccacg accaattttc tcatctactt ctggaatagc gcgtacgtgt gcgggctcac
     2401 cgtcctcctg accatgttgg tgagcgccgc cggggcctac agcctcaccc gctaccgcta
     2461 ccccggtcgg gagcagatcg ccggcctcat cctctgtacg tacatgttcg cccccatcat
     2521 ggtggtgatc ccgttcttca tcctcgtgaa gaagatcggc atcgagaaca cgcacttcgc
     2581 cctggtgctg gccctgacct cgttctgtct ccccttcacg ctctggctgc tgcgggcctt
     2641 cttccagacg atccccatcg agctggagca ggccgcgatg gtggacgggg ccgggcggct
     2701 gcgtgcggtg atctacgtgg tcctgcccct ggcgctgcca gggatcatcg ccacgtccat
     2761 cttcactttc atcctcgcct ggaacgacta catcttcacc cggatcctca tcacctcgga
     2821 cgaactcaag accctgccgg ttggcgtcca ggatcttttc cactcggccc tcgttgactg
     2881 ggggttgatc atgggtgccg gcatgatgat cacgatcccg gcgctgctct tctttgtgac
     2941 cgtccaacgc tacctcatcc gtggctgggg cgccggcgcc gtgaagggct gactcccaag
     3001 aaagcagaca gacaacatgg ccgcctttcg attcgtgctc ctggggacgg gcttttatgc
     3061 gcggaaatgg ctggagctgc tgaagtcccg cgcggactgc gaggtggttg gcatcgcctc
     3121 gcgcttggcg gcccgggcgg aggaattgaa gcgggatttc gagctccccg gggccgccgt
     3181 ctatccgggg tgggaggcgg gctccgccgc ggggagcgcc gacggcgtgc tgatcgctct
     3241 tccccagttg tttcatcctc cagcgatcat ccgcgccctg caagccggtc tccacgtgct
     3301 cgtggagaaa ccgttgagcc tggatatggc gagcgcgcgc gccgtgtacg aggaggcggg
     3361 ccgtcacccc ggcctggctg tcatggtgaa ccagaactat cgctggcgtc cccatatccg
     3421 ggcacttcgc ggggccatcc gagaggggct ggtcggccgt gtcggccaca tccagatcga
     3481 ggttcgccag cagatccgac gcacgacggt gggcggctgg cgcgagcaga tggccgagcc
     3541 attcctcctc gatttcgcca ttcatcactt cgacttgatc cgccatctga ccggcgacga
     3601 ggcgacgcgg gtgctgggga cgagcttccg gccctcctgg agctggttcg acggcaattc
     3661 cgccgccgcc gccatcctca gcatgcgcag cggctgcgtg gtggacttcg gagggacgat
     3721 ggtgtcccag ggtcatgaga caacccagga gggcgtgatc acggtcatcg gggagaaagg
     3781 gacgctgcaa ctggacggag aatcgcaggt gagtctccac ggccagggag acgcgcgcgc
     3841 ccttccgcaa gacccggtgc ccggcggcga gctaggctac gccatgacgg aattcatggg
     3901 ggccatccgg gagaaccggc aacccgagac caatgtagcc gagcatctcc gaagcctggc
     3961 gcttcccctg gcggtcgtcg agtccgctcg ccggaaggcc ccagtggaga tggcggacct
     4021 gctcagcttc ctgtaagttg ctgtggacag tctgggagta tctgattcgg cggtagcgga
     4081 aacgcatcgc ccgaaggact catcaatgtc ggcccagaac ctcagtgtgc tggaagcgtt
     4141 gacccgccac cccctgctcc tcctgccaaa ccgcgtgtat cgcgtgtgga caggaggggc
     4201 cctgctggac cggcttcaag gcaagcccga tcccgaggat tctcacttcc cggaggagtg
     4261 ggtggggtcc acgacgatga cgggcctccc cggccggcct gcggaggagg gcttgagccg
     4321 ggttcgcctc ccggacggat cgacgcagct tctgaagatg ctaatcgagg cccaccccga
     4381 tgccatgctc ggagcggcgc atgtggcacg atacggcgcg gagctgggcg tgctctgcaa
     4441 gctcctggac gcgggaatgc gtctgtcgat ccaggcgcac ccgaaccgcg acttcgcgag
     4501 ggctcacctc cactctgagt tcgggaagac ggagtcctgg ttggtcatcg gcacgcgagt
     4561 gatcaacgga gaacccccct gcatcctctt cggctttcgc gacggggtga cggaggagga
     4621 gtttcgccga atcacccgag cgcaggacac ccagaagcaa gtcgaggcgc tcaaccgcct
     4681 cgcggtccgg cagggcgagg tgtacctggt gcctgcgggc accccgcatg ccatcgggtc
     4741 gggcgtcttc gtggtggagg tgcaggagcc gacggattac gtggtcaacg cggagtacac
     4801 ccttggggcg atccggcgga ccgaggctca atgctacatg ggcctcggct ttgacctggg
     4861 gatgcagtgc ttcgattacc aggcggtggg gatggacttc gttcggcagc acaccttggt
     4921 gccgcgcatc ctccacgagg acgatcaggg tcgcgaagaa atcctcatcg ggccggagga
     4981 cacgccctgc tttggtgcgg tgcggctcgg ggtccggggg gcggtcccgg atcgggaccg
     5041 ggggcgctgc tacgtgggga tcgtgatcga ggggcaggga cggctcctcg ggaccgacat
     5101 cgccatgccg ctccgcgcgg gcgtcacatt cttcgttccc gccgcctcgc ggcatgaggg
     5161 ctatcaggcc gcagccgact tcccgctcac cctgatcaag tgcttcccgc cgcccccctg
     5221 atcccagcca ggtgcttgcc tggattaggg actcctcgcg ggggtagcca cgggtgcctg
     5281 ggcggggccg cgatggcgtt ctcgctcccg gtccaagcgg tccacgtagg cccgaaagcg
     5341 ggcttgctgg tctgcgtcca agacagtctg gatgcgttcc cgggtgcggt tccggatctc
     5401 gaggaagcga ggcctcattt cctcgcgcag ccgagtgaac tggtcgccgg cctcgcgcag
     5461 gatggcctca acttgctgtt gctgttccgg acgcaggtcc agttcgcacg tgaggcgccg
     5521 aatcacgacc tctcggaagc gcgtcgcatc ctggggcggt ccccaccacc acgcgtgcga
     5581 acggtacagc gcaaacccga gcgcacccgt tgccgtgccc agcaggaatg ccaaaagcaa
     5641 gaggagtgcg cccttcaccc gggggctcat agctcgccct gaccaaacac tgccgttagg
     5701 atcaggtcca gatcaggtgc ggggctctcc agcaccagtc gttcactggt ggacaggttc
     5761 tccgtcggca ggaaccctga gggactgggt gcagggctgc cctgaaaaaa gaggaacagg
     5821 aaaacggctg cggtcgccgc gaaggccggg accaatcccc aggccggccg ccacaaatcc
     5881 atctcgacag gcgccggtgc cgggttgcgt ggcagggagg ccagcacttt ctctgcgaat
     5941 ccgacggggg gctccacgcc ctccgtcgcc gctgccacta ggccccggac gaggcgctca
     6001 cgagcgagga gtgcggagca cgccgggcat cccgccagat gaagcctcac cgtctccggc
     6061 aattcccccg cgcggaccgt cgccaattcc tcgagccgtc gcttcagttc cccacacgat
     6121 cccatcggag ccctctcatt ccgaaccctt cattcgattc agcaaaccct cgacggtctg
     6181 tcgcatggcg cgccgccccc gatgcaaccg gaggcggacc gccagtgccg agcaccccat
     6241 ggtctctgcg acctcggccg tcgtatagcc ctggaggtgc gtcagaatca gggcctgccg
     6301 gtctttgggg gccagacacg ccagggcgcg ttccgccaga tcgcgggcgg cggtctgatc
     6361 ctctggttgg gccccccgca tcagatcctg agcggctcgc tgctccccag gggccagatc
     6421 ctcccatgcc gtctcgctgg cgcgtcgcct ttgccgcagc cggtcgtagc ttgcccgggt
     6481 ggcgatgcga atcatccacg gggcgaatgg ggcgccagga tggaagccgg acatcgccgt
     6541 aaaggccttc acgaacgcct cctgcgccac atcctccaca tctgccgggt tgcggagaaa
     6601 ccccccaacg acccgaaaga ccagccgctg atatcggcgt accaaagcac cgaaggcctc
     6661 ccgatccact tcgcgagccc tttggaccag tgtcgcctca tcggggtctt ccgccccctt
     6721 ctgccgcccg tccggttcgg ccatcggctc cttgctaggt tacgctgaag ggcagtccct
     6781 tgtttcagca gccagcttcc tgacgtattc gctggaagcc gtccagcgag ggagcgcgga
     6841 gagcctggct agccgtcaaa ccacggtaat tcagacactt acgatatcac agacaagaag
     6901 ggaggcgcag aacgtaccgg cctcagccga aggatagccc ggccagtcgc gccagcagca
     6961 cgagcgcgat aaggagaccg agtcccccga gcagccaggg tatcaggcgg agacgctgct
     7021 cccaccgtag tcggcggtga acgcgggcac ggcgccgctc ttcctcctcg agttttcgta
     7081 gctcactggg cgggcgaggc atgggcacac aactccattt gtcgcatcat gccagggcaa
     7141 ttcaccgggg tcaaccccaa tggagagacg actccagaca gagccaaggg cctcgagccg
     7201 agagccaggc gattgaagag caatgaaggg ccgcaggcca tgcccgcgat gagaccggcg
     7261 gccctgcctg tgggaaatgc tacgcttacg gcacgacacc gaccgaaaga atcgggtcgc
     7321 ccttgttgat ggcaggagag gtgacgagaa acaggacaat gcccgcggcc gggg
//
LOCUS       DBNGZC010000216         2623 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1485156, whole genome shotgun sequence.
ACCESSION   DBNGZC010000216 DBNGZC010000000
VERSION     DBNGZC010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1485156"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="AC16G0_03935"
     CDS             complement(<1..287)
                     /locus_tag="AC16G0_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006271262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HIR5098806.1"
                     /translation="MERDPRTFVIIGAALEVHRELGAGFLEAVYQEAFARELTKRSVP
                     FRAEVEIPVFYKGEQLSTAYRADFVCFDSIIVELKALTRLTGVEESQVINYL"
     gene            complement(333..1433)
                     /locus_tag="AC16G0_03940"
     CDS             complement(333..1433)
                     /locus_tag="AC16G0_03940"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009100657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HIR5098807.1"
                     /translation="MASYRITVIPGDGIGPEVMAEALKVLRASETVFPGLRFECKEYP
                     AGARCYKETGSDLPAATLEACRNADAILFGSTGDPSIRFADGTEIAPQLTLRFVLDLY
                     AGIRPIKRYAGVPPTLAGDPAIDYVIMRENCEGLYASRGAGVRVGEHVAVDNMVITRS
                     GTERIVRYAFRLAEQRRGAPADKTRRVTCVDKANVLKSMAFFRQIFYEVAREFPGTKT
                     EHVYVDAMTIYMVQRPLHFDVVVAENMFGDIISDLAAGTMGGLGLAPSADVGDTYGLF
                     QPSHGTAPDIAGKGIANPIAQILSAGMMLTWLGERKNDPQARDAAMAIERAVEETLAD
                     HRYHTADLGGQAPTKLVGDTIASRVGKNKTHN"
     gene            complement(1501..>2623)
                     /locus_tag="AC16G0_03945"
     CDS             complement(1501..>2623)
                     /locus_tag="AC16G0_03945"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5098808.1"
                     /translation="DLLILDGRIASVGTTLPAPEGVGEGEVIGVAGRLILPGLIDGHI
                     HVMGASGMGGPTTRTTDLQIERIACAGVTTVVSPLGADSLSRTIPALLARATALTCEG
                     ISAYCYTGGWRRPVPTLTGDPQADVAFIDRVLGVKVAISEPLAPVYSVKELCGLAHAA
                     YTGGRLAGKDAVLHTHIGDLPDGLQPLQEVQRRTGIPADRMVATHVNRNPHLWKQALE
                     YARMGGSIDLTAMQRPETGHPEATPAAAAIREALTAGIPPSRLTLSTDSGAAYPRYDI
                     QGHAAGQYMPGPASLLTTMRELVQDGLTWGQAVSYATRHTAERLGLRLKGRLAEGCDA
                     DVLVLTSAGDVDRVYARGRPLVEQGAPMVRGPFDGHASL"
BASE COUNT          416 a          894 c          839 g          474 t
ORIGIN      
        1 aggtagttga tgacctggga ctcttcgact cctgtgagcc gggtgagggc cttgagctcc
       61 acgataatcg agtcaaaaca tacgaagtca gctcgatacg ccgtggacag ttgctcgcct
      121 ttgtagaaga ctggaatctc tacctcggcc cgaaatggaa cgctccgctt ggtcagttcc
      181 ctagcgaaag cctcctgata gacggcttcc aggaatcccg ctcccagctc tcgatggacc
      241 tccagagctg ctccaattat gacgaaagtc ctaggatccc tttccacgat cctccgcctc
      301 agtgaatcgg tgtaatctgc gtaatctgcg gattaattat gggtcttgtt ctttccgacc
      361 cgtgaggcga tggtgtcacc tacaagcttc gtcggggcct gacccccgag gtcggcggta
      421 tgataccggt ggtcggcgag cgtctcctcc accgctcgct cgatggccat ggccgcatcc
      481 cgggcctggg ggtcattctt ccgctcgccg agccacgtga gcatcatacc agccgagagg
      541 atctgggcaa tcgggttggc gattcccttg ccggcgatgt ccggcgccgt cccatgggat
      601 ggttggaaca ggccgtaggt atcccccacg tccgcagagg gcgccagacc caagccgccc
      661 atggtccccg ccgccagatc cgagatgata tcgccgaaca tgttctcggc cacgacgacg
      721 tcgaagtgga gcggtcgctg caccatgtag atcgtcatgg cgtccacata gacgtgctct
      781 gtcttcgtcc cggggaactc ccgcgccacc tcgtagaaga tctgtcggaa gaaggccatg
      841 ctcttcagca cgttggcctt gtccacgcag gtgacgcgcc gcgtcttatc ggcaggcgcg
      901 ccgcgccgct gctccgccag ccggaaggca tagcgcacaa tccgctcggt cccggagcgg
      961 gtgatcacca tgttgtccac ggccacgtgc tcccccaccc gcacccccgc cccccgagag
     1021 gcgtacaggc cttcgcaatt ctcccgcatg atcacgtagt cgatggccgg gtccccggcc
     1081 agcgtgggcg gcaccccggc gtagcgcttg atcggccgga tccctgcata cagatccaag
     1141 acgaagcgga gggtgagctg cggcgcgatc tcggtcccgt cggcgaatcg gatgctgggg
     1201 tcgccggtcg agccgaaaag gatggcgtcc gcgttccggc aggcttccag cgtcgcggcc
     1261 ggcagatcgc tgcccgtctc cttgtagcag cgcgcgcctg caggatactc cttgcactcg
     1321 aacctgaggc cggggaagac tgtctccgag gcgcggagga ccttgagggc ctccgccatg
     1381 acctcgggcc cgatgccgtc tcccgggatg acggtgatgc gataacttgc catgttggat
     1441 atccttttct cttggtttcg tcagaacctt tgcgtccttc ggccccgcgc cccgtctacg
     1501 tcaaagagag gcgtggccgt cgaaagggcc ccgcaccatc ggagcccctt gctccaccag
     1561 aggacggccg cgcgcgtaga cccgatcaac gtcgccggcc gacgtgagca ccagcacatc
     1621 ggcgtcacag ccctcggcca gcctcccctt cagccgaagc cccaggcgct ccgccgtgtg
     1681 tcgcgtggcg taggataccg cctgccccca ggtgagaccg tcctgcacca gctcgcgcat
     1741 ggtcgtgagg agcgaggccg gcccgggcat gtactggccc gccgcgtgcc cctgaatgtc
     1801 ataccgaggg taggcagctc cgctgtcggt cgagagcgtg agtctcgatg gtggtattcc
     1861 cgcagtcagg gcctcgcgga tggcggctgc ggcaggcgtg gcctcagggt gtcccgtctc
     1921 cggtcgctgc atcgcggtga ggtcgatgct tccacccatc ctcgcgtatt ccagggcctg
     1981 cttccacagg tgggggttgc gattcacgtg ggtggctacc atccggtctg ccgggatccc
     2041 ggtccgccgc tgcacctcct gcaggggctg gagcccatcc ggcaggtcgc cgatatgagt
     2101 gtggaggact gcatccttcc ccgccagtct cccgcccgtg tacgccgcgt gggcgagtcc
     2161 gcacagctcc ttcaccgagt acaccggggc caggggctcc gagatcgcga ccttgacccc
     2221 caggactcga tcgatgaagg ccacgtctgc ctgcgggtcg cccgtgagcg tcgggaccgg
     2281 tcgccgccac cccccggtgt agcagtaggc gctgatcccc tcgcaggtga gggcagtcgc
     2341 ccgcgccaag agcgcaggga tggtccggct gagactgtcg gccccgagag gggagaccac
     2401 ggtggtgacg ccggcgcagg cgatgcgctc gatctggagg tcggtggttc gggtggtcgg
     2461 cccgcccatc ccactcgccc ccatgacgtg gatgtggccg tcgatcaggc cgggcaggat
     2521 gagccggcct gccacgccga tgacctcccc ctcgccgacc ccctcgggag ccgggagcgt
     2581 ggtcccgacg gaggcgatcc ggccgtccag gatcagcagg tcc
//
LOCUS       DBNGZC010000217         1539 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1092885, whole genome shotgun sequence.
ACCESSION   DBNGZC010000217 DBNGZC010000000
VERSION     DBNGZC010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1092885"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="AC16G0_03950"
     CDS             <1..221
                     /locus_tag="AC16G0_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HIR5098809.1"
                     /translation="WAAAVERALPPIAIGLMAAGVYTVGQSAVTDLPTALLAVAAAIV
                     LARRWLPPAAVVIAGGLLSWLVSAMISR"
     gene            383..1123
                     /locus_tag="AC16G0_03955"
     CDS             383..1123
                     /locus_tag="AC16G0_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012402.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HIR5098810.1"
                     /translation="MAEAPTKSQIFRGRLTEIIPEAREVKTFRFAPEGGVSITHLPGQ
                     FMHISVPIPDGDRPQTRRSFTISSAPTETGRLQITVKRNPAGTVSNFLHQQARVGDTF
                     DLRAPFGRFTFTEGRASRILCIGGGSGVTPLRAILRYICDRQAPVEVVHLDFNRREED
                     IIFRREFQGMPAAHPGVRVHFALTDPGPAWQGLAGRIRADLVDQTLKGFEPEIVYLCG
                     PPAMMDAAKQLLVGRGLRPDQIVTESFG"
     gene            complement(1244..>1539)
                     /locus_tag="AC16G0_03960"
     CDS             complement(1244..>1539)
                     /locus_tag="AC16G0_03960"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR5098811.1"
                     /translation="RAVGFGVGSVVMSFLFEALCIAFVGGLLGCAAVLPLNNLTTGTM
                     NWQTFSHLAFAFRVTPPLLVSGLLFALLMGLVGGVPPAIRAARQPIALALREL"
BASE COUNT          293 a          511 c          485 g          250 t
ORIGIN      
        1 cgtgggccgc ggccgtcgag cgcgccctgc cgcccatcgc catcggtctc atggccgcgg
       61 gcgtgtacac ggtggggcaa tcggcggtga cggatctccc caccgctcta ctggcggtcg
      121 ccgccgcgat cgtcctggcc cgaagatggt tgccgcccgc agccgtcgtg atcgccggcg
      181 ggctcctgag ttggctggtg agcgccatga tttcgcgttg aacttcgggg tgccaatagt
      241 agggtcgcgc ttggggggcg aggcctggac tgatatactc tggtcacacc ctcttcagga
      301 tcttgccgcg ttcatgcaaa aggtggagag ggataggcgg cacacccgga cctggtagag
      361 ttgcgagacg aaggagtcac gcatggctga ggcgccgacc aagtcgcaga tcttccgcgg
      421 acgcctgacg gagatcatcc ccgaagcgag agaggtgaag accttccgtt tcgccccgga
      481 gggtggggtc tcgatcaccc atctccccgg ccagttcatg cacatcagcg tccccatccc
      541 cgatggggac cggccgcaga cccgtcgatc cttcaccatc tcctccgccc ccacggagac
      601 cggccgcctg cagatcactg tcaagcgcaa tcccgcgggc accgtctcca acttcctgca
      661 ccagcaggcg cgggtcggcg ataccttcga cctgcgcgcc cccttcggtc gcttcacctt
      721 caccgaggga cgggccagcc ggatcctatg catcggcggc ggaagtggcg tgacccccct
      781 gcgcgccatc ctgcgataca tctgtgatcg tcaggctccg gtggaagtcg tccatctgga
      841 cttcaaccgg cgggaagagg acatcatctt ccgcagggaa ttccagggca tgcccgccgc
      901 ccatcccggc gttcgagtcc acttcgctct caccgatccg ggccccgcct ggcaagggct
      961 ggccggccgc atccgggccg acctcgtcga tcaaaccctg aagggatttg aacccgagat
     1021 tgtctatctc tgcggccctc cggcgatgat ggacgcggca aagcaactcc tcgtcggacg
     1081 ggggttgcgc cccgaccaga ttgtaacgga aagcttcggc tagctccctg ggggaatgac
     1141 caaatcccaa tgaccaatga ccaaatacgg cgccactttc tgtgaggcaa agtaccggcg
     1201 tgcgccagga tcgcggctcc agtgggaatc gggaagtgat ggctcagagc tcccggagtg
     1261 ccagggcgat gggctggcgg gctgcccgga tcgcaggggg cacgccacca acgagcccca
     1321 tcagcaatgc gaaaagcaat cccgaaacca gcaggggtgg ggtcacgcgg aaagcaaagg
     1381 ccagatggga gaaggtctgc cagttcatgg ttcccgtcgt caagttgttc aaaggcagga
     1441 cggccgcaca gcccagcaga ccgccgacaa aggcaatgca aagcgcctca aagaggaagg
     1501 acatcaccac gctgccgacg ccgaatccta ccgcgcgca
//
LOCUS       DBNGZC010000218         1567 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_409092, whole genome shotgun sequence.
ACCESSION   DBNGZC010000218 DBNGZC010000000
VERSION     DBNGZC010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_409092"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..431
                     /locus_tag="AC16G0_03965"
     CDS             45..431
                     /locus_tag="AC16G0_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098812.1"
                     /translation="MGPITLEVIAPLISGTKHCQHCQVFMDDAGIDQRVQSEELNSYP
                     EEMWRDYTRLSQMVRELSLCYGAGLRIVLIDPHTPMGLWKSLRHWIRRYPAFVLRGQE
                     KYIGWDLEAVDRLLRTGGAVSAPAAS"
     gene            608..868
                     /locus_tag="AC16G0_03970"
     CDS             608..868
                     /locus_tag="AC16G0_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098813.1"
                     /translation="MKAEYLRLSTWVKEMRRTHEGRVVIRILDPQSLFGIWRLLRHRI
                     RRFPTFFVDGAERVVGWEGDPDAAVARALSRRATAATSEART"
     gene            complement(994..>1567)
                     /locus_tag="AC16G0_03975"
     CDS             complement(994..>1567)
                     /locus_tag="AC16G0_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR5098814.1"
                     /translation="VYLVGGSCELPVLARALRERFGRRVRRSPYPPAATAIGLAFAAD
                     QEGGYTLTERFTRHFGVWREGESGEKVVFDPIFTKQTPVPQPGQPALIVRRRYRPVHN
                     LGRFRFLECSSLGEQGGPAGDILSWEEALFPFDPRLVQKEHLEKVPVERTPAVESYAI
                     EEIYRCDSGGVIEVTIANLTTGFAKTYRIR"
BASE COUNT          286 a          520 c          498 g          263 t
ORIGIN      
        1 gggcggggcc agccccgccc gtggcacgtt gttgaaaggg agccatggga cccattactc
       61 tcgaagtcat cgccccgctg atctccggga caaagcattg ccagcactgc caggtgttca
      121 tggatgacgc ggggatcgac cagcgggtcc agtcggaaga actgaactcg tacccggaag
      181 aaatgtggcg ggactacaca cgtctgtccc agatggtccg ggaactgtcc ctgtgctatg
      241 gggccgggct ccgcatcgtg ctcattgacc cccatacgcc catgggcctc tggaagtctc
      301 tccggcactg gattcgtcgc tatcccgcct tcgtcctgag gggacaggag aagtacatcg
      361 ggtgggacct ggaggccgtg gacaggctgt tgcggacagg cggtgccgtc tccgcccccg
      421 ccgcttccta gtctcgatcg aggttcgggg aggggaacac atgtcaaccg gaaggcctgt
      481 cgtggtcgag gtggtagccc ccgttctttc cggattctac cactgacccc actgcgagac
      541 gttcctggat ggagcggggg tcgggaagca ggtccacaca caggagatca acgagtatcc
      601 cgaggacgtg aaggcggagt acctgcgcct gtccacctgg gtcaaggaga tgcggcggac
      661 ccacgagggt cgggtcgtca tccgcatcct ggatccccaa tccttgttcg gcatctggag
      721 gctcctgcgg caccgcatcc gtcgcttccc cactttcttc gtggacggcg cggaacgcgt
      781 cgtgggatgg gagggcgatc cggacgccgc ggtggcccgc gccctcagcc ggcgggccac
      841 ggctgccacc agcgaggcac gcacgtaaac ctcacccaaa ctcgagtccg aatacttgtg
      901 gccgccgatt ctcggcggcc acagtcattc tcgtcctcga gtcagaaaca caaggggatc
      961 cgcgcaggta agtcccccgc aaccctcacc cacctatcga atccggtagg tcttggcgaa
     1021 acccgtggtc agattcgcga tggtgacctc gatcacccct ccggaatcac accgatagat
     1081 ttcctcgatg gcatacgact cgacggcggg cgtgcgctcg accgggacct tctccaggtg
     1141 ctccttctgt accagcctcg ggtcgaaggg aaagagcgcc tcctcccagg agaggatgtc
     1201 acccgccggc ccgccctgct ccccgaggct gctgcactcc aagaaccgga aacggcccag
     1261 gttatggacc gggcgatagc ggcggcgcac gatcaaggca ggctgccctg gctggggcac
     1321 gggcgtctgc ttcgtgaaga tggggtcgaa cacgaccttc tcccctgact caccttcgcg
     1381 ccacacgccg aagtgacggg tgaagcgctc cgtgagggtg tatccaccct cctggtcggc
     1441 ggcgaacgcc aggccaatcg cggtcgcagc cggcggatac ggagaccttc ggacacgcct
     1501 cccgaaccgc tcccgcaacg cccgggccag gacgggcagc tcgcaggagc cgccgacgag
     1561 atacacg
//
LOCUS       DBNGZC010000219         3911 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_312127, whole genome shotgun sequence.
ACCESSION   DBNGZC010000219 DBNGZC010000000
VERSION     DBNGZC010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_312127"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..782
                     /locus_tag="AC16G0_03980"
     CDS             237..782
                     /locus_tag="AC16G0_03980"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR5098815.1"
                     /translation="MAKFLESHRNRWNAWNVPYEDGKILHDLVRVGRSRNILEIGTST
                     GHSTIWLAWAAAKTGGKVVTLEIDPGRHAIAMENFKQAGVETYIDARRADAHDLVKQL
                     AGPFDFVFNDADKEWYLNYFTDLDAKVAVGGCFTAHNTLRIYAKEVRDFLDYVRAKPN
                     YQTRIERGSGEGISISCKTAP"
     gene            1070..1534
                     /locus_tag="AC16G0_03985"
     CDS             1070..1534
                     /locus_tag="AC16G0_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098816.1"
                     /translation="MRKALVLLAVLLVLFGTMLPSPVLADGGWHGGHHGIHGGWWWPG
                     AVIGGLVVGALAIATAPLWALSAPPVYSPPVVYAPPVSYAPAPAYSAPPTYAPPPPTY
                     QPPGVSAPPRAPAVQREVVYPNGRYVLYGDGIRQAWRWVWVPSASPPPPPPR"
     gene            complement(1600..2889)
                     /locus_tag="AC16G0_03990"
     CDS             complement(1600..2889)
                     /locus_tag="AC16G0_03990"
                     /EC_number="5.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF021387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase family
                     protein"
                     /protein_id="HIR5098817.1"
                     /translation="MAKADDWHVERIPYGKQEVEIKIPTRNYLATLAPRYRPGLEDTP
                     AAIRHALAHPIGSRRLRDIARGKQSAVIVVNDVTRPTASDKLLPPLLEELREAGLGNE
                     QILFLVATGTHRDNTREELERMLGKRILDEFRVLNHHCQDEENMLDLGKTRDGIPVVI
                     NGLFCQAAVKILTGTIGPHHGAGFSGGRKSLLPGLASLSTLKRHHGFHMRSPKPAMGW
                     VEGNRFHEAALEAARMAGVDFILNTVQNDRKEISHVVAGDLEQAWLDGVRASREIFEV
                     RVPEPAEIVITSPGGYPKDINLWQSQKAMSAAELVVKDGGTVILSAECPDGVGSRGFY
                     EWMSTATCPQDVIDRFIREEYSVGSSKAWMYSRCLKRAELIVVTQGIDDQTLQHMFTK
                     RADTVEQAIALALARHGENARIILLRNGSDLIPRFEL"
     gene            complement(2966..3379)
                     /locus_tag="AC16G0_03995"
     CDS             complement(2966..3379)
                     /locus_tag="AC16G0_03995"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5098818.1"
                     /translation="MAKAHIFQFDTIPTVDRGEGVTTKPLVSKAVGSTKLTNGVTTFP
                     PGGALRLHSHNCDESVTLIVGEGVVEIEGKQTPMKPFDTTFVPAGVVHRFLNKSAKPM
                     SILWNYTSVDVTRTFTDTGETVGQLSPTDKGGTRA"
     gene            complement(3457..>3911)
                     /locus_tag="AC16G0_04000"
     CDS             complement(3457..>3911)
                     /locus_tag="AC16G0_04000"
                     /inference="COORDINATES: protein motif:HMM:NF040043.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HIR5098819.1"
                     /translation="PDLIEILRLIAEADIILATGHLSVREIKTVVDAAVATGVRKILV
                     NHPDLWLIGMSIEDERELAAKGAMLEMCIRSVTAPGHGETSPRFLADRIKAVGAAHVV
                     MATDYGQVDSPPAPEGMHWYIAQMLECGIPPKDVELMTKVNPARLLNL"
BASE COUNT          645 a         1289 c         1209 g          768 t
ORIGIN      
        1 cccgtctccc ccacgggaga gggggcggtg acgggggcga ggggcaccgc tggccgcagc
       61 acttgcgaga gcgagggcgg gctgtgggaa gacttcgagc ggcgcgtgtg caagccaggg
      121 gatgcggcat caagtgtcga tggcgtgacg ggcatgtaat tgttgcagca ctgacactgg
      181 cgttgctcgg ttatgcctcg caaggacagg cccaacagcc gtcactggat tccagggtgg
      241 cgaaattcct agaaagccat cggaaccgct ggaacgcctg gaacgtcccc tacgaggacg
      301 ggaagattct gcacgatctc gtccgggtag gccggtccag gaacatcctg gagatcggca
      361 cctcgaccgg ccattccacc atctggctgg cctgggccgc cgccaagacg ggcgggaagg
      421 tcgtcaccct cgagatcgat ccgggtcgcc acgcgatcgc catggaaaat ttcaagcagg
      481 ccggcgtcga gacctacatc gacgcccgac gggccgacgc tcacgacctc gtcaagcagc
      541 tcgccggccc attcgacttc gttttcaacg acgccgacaa ggaatggtac ctcaactact
      601 tcacagatct cgacgccaag gtcgccgtgg gtggctgctt caccgcgcac aacaccctgc
      661 ggatctacgc caaggaggtg cgagacttcc tggattacgt gagggccaag cccaattatc
      721 agacgcgcat cgagcgagga agcggtgaag ggatttccat cagttgcaag acagctccct
      781 gaaaggctgc tgagaaggcc catctgccgg gtacccatcc gtcggatgga tgcccacccc
      841 tggcgggtac cccccgcgct cctgcagtgc tgcagcgtgc ggatgaaaca tgccatgcac
      901 ctcacaccgc ctttcacctg gccaagttct cataggttgg agtatactca ttcggtcgag
      961 ggcggctcgg tttggccatg acttcgtgac gcgccctggg ccgctcagcc ttatggcaat
     1021 tgcatatcag ccagctaggc catctactcc gacctcggag ggaatgatga tgaggaaagc
     1081 actcgtgctg ctcgcggtac ttctcgtgct ctttgggacg atgctcccga gcccggtctt
     1141 ggcggatggc ggatggcacg gcgggcacca tggtattcac ggcgggtggt ggtggcccgg
     1201 ggccgtcatc ggcggtctcg ttgtcggcgc cctcgccatc gcgacggctc ccctctgggc
     1261 gctgtccgca ccgccggtgt actccccccc cgtcgtatat gcgccgcccg tgtcgtacgc
     1321 cccggcgccg gcgtattcag ctccgccgac gtatgctccg ccgccaccga cttatcagcc
     1381 gccaggggtg tccgcgcctc cccgggcccc cgcggtccag cgcgaggtcg tctatcccaa
     1441 cggtcggtat gtgctctacg gcgacggaat ccgccaagcc tggcggtggg tctgggtgcc
     1501 gtcagcgtcg ccgcctccgc ccccgccacg gtagtcatcg ggcgcgcccc agaggaccac
     1561 gggaagctcg cgggtcacgg tcagcgcttc tgccggatct catagttcga atctagggat
     1621 cagatccgat ccgttgcgta acaggatgat ccgggcgttc tcgccgtgcc tggccagcgc
     1681 caaggcaatt gcctgttcga cggtatccgc ccgcttcgtg aacatgtgct gcaaggtctg
     1741 gtcgtcgatg ccctgggtga cgacgatcaa ctccgcccgc ttgagacagc gggagtacat
     1801 ccatgccttg gaggagccga cggaatactc ttcccggatg aaccgatcga tcacgtcctg
     1861 cgggcaggta gccgtcgaca tccactcgta gaagccccgc gagccgaccc catccggaca
     1921 ttctgcggac aggatcaccg tcccgccgtc tttcaccacg agttcagcgg ccgacatggc
     1981 tttctgggat tgccagagat tgatatcctt cgggtatcct cccgggctgg tgatgacgat
     2041 ctccgccggc tcaggcacgc gcacttcgaa tatctcccgg ctggcccgaa ccccgtccag
     2101 ccaggcctgc tccaggtcgc ctgccaccac gtggctgatc tccttccggt cgttctgcac
     2161 ggtgttcagg ataaagtcta ccccggccat cctggcggct tccagcgccg cctcgtggaa
     2221 ccggttgccc tccacccaac ccatggccgg cttgggagaa cgcatgtgga acccatggtg
     2281 ccgcttcagc gtcgagagac tggccaggcc tggcagcaga ctcttcctgc cgccggaaaa
     2341 gccggcgccg tggtgggggc cgatcgtccc ggtgaggatc ttcaccgccg cctggcagaa
     2401 gagaccgttg atgacgacgg gaataccgtc gcgagtcttc cccaggtcga gcatgttctc
     2461 ttcgtcttgg cagtggtggt tgaggacccg gaactcatcc aggatcctct tccccagcat
     2521 tcgctccagc tcctcccggg tattgtcccg gtgcgttccg gtcgcgacca ggaagaggat
     2581 ctgctcgttc ccgaggccgg cctcccgtag ctcctctagg agcggcggca ggagcttgtc
     2641 gctggctgtc ggccgcgtca cgtcgttcac gacgatcacc gcgctctgct tccctcgggc
     2701 gatgtcgcgg agccgccggc ttccaatcgg atgtgccaag gcgtggcgga tcgcggcggg
     2761 agtatcctca aggcccggcc ggtacctggg cgccagcgtg gccaggtagt tcctcgtggg
     2821 aatcttgatt tcgacttcct gcttgccgta cggaattctc tctacatgcc agtcgtcggc
     2881 ctttgccatg cctcgcgtct ccttcctcgc ctatggtcac caggccccgg ccctggaagt
     2941 tccctttgct ctcgtccaga gggcgtcagg cccgggtacc gcccttgtcc gtcgggctca
     3001 attgcccaac ggtctctccc gtgtctgtaa aggtccgcgt gacatccacc gaggtgtagt
     3061 tccagaggat gctcatgggc ttcgcgctct tgttgagaaa gcgatggaca acgccggccg
     3121 gcacgaaggt cgtgtcaaag ggcttcatgg gcgtttgctt tccctcgatc tcgaccaccc
     3181 cttcgcccac gatgagggtg accgattcgt cacagttgtg tgaatgcagc cggagcgctc
     3241 cgccgggagg gaacgtggtc accccgttcg tgagcttggt ggagcccacg gccttgctga
     3301 ccaggggctt ggtggtgacg ccctcacccc tgtccacggt cggaatcgtg tcgaactgga
     3361 agatgtgcgc tttggccatt ctcgtcctcc caggctagtg gcgtgcccat tcaggtgtcc
     3421 tgagcgcccg atcccggatt cctcggtgcc cagcgctcaa agattcagaa gtctggcggg
     3481 gttcaccttg gtcattagtt ctacgtcttt cggaggaatc ccgcactcca gcatctgcgc
     3541 gatgtaccag tgcatcccct ccggagctgg tggactgtcc acctgaccgt aatccgtggc
     3601 catgaccaca tgcgcagccc cgacggcctt gatccgatcg gcgaggaacc tgggtgaagt
     3661 ctcgccgtgt cctggagcgg tgacgctgcg gatgcacatc tccagcatgg cgcccttggc
     3721 ggcgagctcg cgctcatcct cgatgctcat gccgatcagc cagaggtccg gatgattcac
     3781 gaggatcttc cgcacacccg tggctacggc ggcatccacc acggtcttga tctctcgcac
     3841 cgacaggtgg ccggtcgcca ggatgatgtc ggcctcggcg atcaggcgca gaatctcgat
     3901 caggtcgggc a
//
LOCUS       DBNGZC010000220         3610 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1387970, whole genome shotgun sequence.
ACCESSION   DBNGZC010000220 DBNGZC010000000
VERSION     DBNGZC010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1387970"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..1161
                     /locus_tag="AC16G0_04005"
     CDS             211..1161
                     /locus_tag="AC16G0_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098820.1"
                     /translation="MIRRVAISVVTLFLITLIVFTGVRMIPGDPARVMAGTDADEAGL
                     QEVREKYGLNDPIPMQYLRWIRLALHGDLGESIRTREPVVKTVATKLPITIQLACFSL
                     IIAIGIAIPAGIFSALRRNTIWDLLANAVSLCGVSIPTFWLGIMLILFISVRLHLLPA
                     SGFVPFFQDPLGNLERMIMPAFVLGGALAAVLMRQTRNSMIEVMSANYIRTAYSKGLA
                     EPRVVLRHALRNGLIPIVTILGLQMGALMGGAVVTEQIFVVPGFGRLIVEAVFTRDYP
                     LVQGVVMITASSYVLINLLVDVSYSVLNPRIRVKGSASES"
     gene            1175..2059
                     /locus_tag="AC16G0_04010"
     CDS             1175..2059
                     /locus_tag="AC16G0_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098821.1"
                     /translation="MTGSGEGLDQDGAPAASPRSRRTWRRALRRPPAFIGAVVVLLFL
                     VMAVGAPWIATSDPLRTNWSQIRKPPTLAHLFGTDDLGRDVYSRVVWGARISMQAGVF
                     SILLAIAVGVPTGLVAGYYRGIIDQAIMRLTDAWLSFPFLILAIGLVTILGTSLTNAT
                     VAIGLAATPTYIRLTRGLVLSTAEEDYVQSARALGGGDVRMMLRHILPNIFSALLVQA
                     TVSIPGAIIAEAILSFLGLGVQPPAPSWGTMLNTAQQFLETAPWMSWWPGLAIFCLAL
                     SFNLAGDGLRDFLDPKDY"
     gene            2112..3386
                     /locus_tag="AC16G0_04015"
     CDS             2112..3386
                     /locus_tag="AC16G0_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HIR5098822.1"
                     /translation="MSRASIQQHVKQAVWSQPVSDLHTHSYPPAFGASPAPGALLLWG
                     VDELVTYHYLIAEVFRVVPATELPYETFWAMSKQQQADHIWKYLFVKRTPIGEACRGV
                     LTALKRLGLDPNEKTLTPYRKWFAKQDPDKYIDKVMKIANVDLITMTNPVFDDHERSL
                     WEKVPGAGADPRFAAVLRIDPLLRDWRGACEKLQAWAYDARPDFSGKTVEEVRRFLAY
                     WLEKMKAVYVAMSLPPEFRYPDLTNPAGDRFIREILMPVLADRGKPWAMMIGARLRVN
                     PGLREGGDTVGTADVMSVVNLCQEFPQNKFMVTILARENQHELAASARKYGNLLPFGC
                     WWFLNNPLLVEEITRMRLEILGTTFVPQHSDARILDQLIYKWEHSRRVIAKVLTDKYV
                     DLEASGFKVTKQHIQSDVKLLLRDNYRSFLER"
BASE COUNT          608 a         1190 c         1135 g          677 t
ORIGIN      
        1 caagatcgtt ctgggccggc ggaacctgat ctatctttat caccagaact acatcgtggc
       61 ctacccgaag aatctgacga actacaaggc tgtgccggac ggcctgatcc gcatcaaggg
      121 cgtgtcctgg aagtaacccc aaagggcgcg accccgtgtc gcctgggaca caggcggcac
      181 agggtcgcct gatcactcgt gcttgatttc ttgattcggc gggtcgcgat ctccgtcgtc
      241 accctcttcc tgatcaccct gatcgtcttc accggggtcc gcatgatccc cggcgatccc
      301 gcccgggtga tggccgggac ggacgcggac gaggctgggc tccaggaggt ccgggagaag
      361 tatgggctga atgatcccat ccccatgcag tacctccgct ggatcaggct ggcgctgcac
      421 ggcgacctgg gcgagtcgat ccgcacgcga gagccggtcg tcaagacggt ggcgacaaag
      481 ctgccgatca ccattcagct tgcctgtttc tcgctgatca ttgccatcgg gatcgccatc
      541 ccagcgggga tcttctcggc cctgcgccgc aacacgatct gggatctcct ggccaacgcc
      601 gtgtccctct gcggggtatc catccccacc ttctggctgg ggatcatgct gattctcttc
      661 atctccgtac ggcttcacct cctgcctgcc tcgggcttcg tgccgttctt ccaggacccc
      721 ctgggcaacc tcgagcgcat gatcatgccc gcattcgtcc tcggcggcgc cctggcggcg
      781 gtgctgatgc gccagacgcg taacagcatg atcgaagtca tgagcgccaa ctacatccgg
      841 accgcgtaca gcaagggcct ggcggaaccc cgggtcgtgt tgcgtcatgc cctcaggaat
      901 gggctcatcc ccatcgtcac gatcctggga cttcagatgg gagccctcat gggtggtgcc
      961 gtcgtcaccg agcagatctt cgtggttccc ggtttcggac ggctgattgt ggaggcggtg
     1021 ttcacccggg attaccccct ggtccagggc gtggtcatga tcacggcgag cagctacgtc
     1081 ctgatcaacc tcctggtgga tgtgtcgtat tccgtgttga acccacggat ccgcgtcaag
     1141 gggtcggcca gtgagtcgtg acggcgggat cgtgctgacg gggagcgggg aagggctcga
     1201 ccaagatggc gcgcctgcgg cgtctcctcg gtctcgtcgg acctggcggc gcgccctgcg
     1261 ccggccgccg gccttcatcg gcgccgtcgt ggtgctgctg ttcctggtga tggccgtcgg
     1321 cgcgccgtgg atcgcgacct ccgacccgct ccgcaccaat tggagccaga tccgcaagcc
     1381 tccgaccctg gcgcacctgt tcggcacgga cgacctgggg cgggatgtct actcgcgggt
     1441 ggtgtggggg gcccggatct cgatgcaggc gggcgtgttc tccatcctcc tggccatcgc
     1501 cgtcggggtg ccgaccggcc tcgtggccgg ctattaccgc ggcatcatcg accaggcgat
     1561 catgcggctc accgacgcct ggctgtcgtt ccccttcctg atcctggcca tcgggctggt
     1621 cacgatcctg ggaacttccc tcaccaacgc caccgtggcc atcggcctgg ccgccacccc
     1681 cacctacatc cgcttgaccc gtggactagt gctctccacc gcagaggagg actacgtgca
     1741 gagcgcgcgg gccctcggcg gcggggacgt gcgcatgatg ctgcgtcaca ttctcccgaa
     1801 catcttcagc gctctcctgg tgcaggccac ggtttcgatc ccgggggcga tcatcgcgga
     1861 agccatcctc tcctttctcg ggctgggcgt gcagcccccc gcccccagct gggggaccat
     1921 gctcaacacc gcccagcagt tcctggagac ggccccctgg atgtcctggt ggcccggcct
     1981 ggccatattc tgcctggcgc tctcgttcaa cctggccggc gacggcctgc gggactttct
     2041 cgatcccaag gactactgat cgtctctggc acagccgccg tcgccctgcc caactgagga
     2101 ggatccgaac catgagccgc gcgagcatcc agcagcacgt caagcaggcg gtctggtccc
     2161 agccggtgag cgacctgcac acccactcct acccgcccgc cttcggggcc tcacccgcgc
     2221 cgggggcgct actgctctgg ggggtggacg agctggtgac ctatcactac ctcatcgccg
     2281 aagtgttccg cgtcgttccc gccaccgagc tcccctacga gacgttctgg gcgatgagca
     2341 agcagcagca ggccgaccac atctggaaat acctgttcgt gaagcgcaca cccatcggtg
     2401 aggcctgccg aggggtgctc acggcattga agcggctggg cctggacccc aacgagaaga
     2461 ccctcacgcc ctaccgcaag tggttcgcca agcaggatcc ggacaagtac atcgacaagg
     2521 tgatgaagat cgcgaacgtg gacctcatca ccatgaccaa ccccgtcttc gacgaccacg
     2581 agcgctcgct ctgggagaag gtgcctggcg ccggcgccga tccgcgcttc gcggccgtgc
     2641 tccggatcga tcccttgctg cgggactggc ggggggcctg cgagaagctc caggcatggg
     2701 cctacgatgc ccggccggat ttttcgggca agaccgtgga ggaggtgcgg cgcttcctgg
     2761 cgtattggct cgaaaagatg aaggcggtct acgtggccat gagcctcccg ccggagttcc
     2821 gctatcctga tttaaccaat ccggccggag accgcttcat ccgggagatc ctcatgccgg
     2881 tgctggcgga ccgcggcaag ccctgggcaa tgatgatcgg cgccaggctg cgggtaaatc
     2941 cagggctacg ggagggcggc gacacggtgg ggacggcgga tgtgatgtcg gtggtgaacc
     3001 tctgtcagga gttcccgcag aacaagttca tggtcacgat cctggcgcgc gagaaccagc
     3061 acgagctggc agcttccgcc cggaagtacg ggaacctcct tcccttcggc tgctggtggt
     3121 tcctgaacaa cccattgctg gtcgaggaga tcacccggat gcggctggag atcttgggga
     3181 cgaccttcgt cccgcagcac tccgacgcgc gcatcctgga tcaactgatc tacaagtggg
     3241 agcacagccg gcgggtcatc gccaaggtcc tcacggacaa gtatgtggac ctggaagcct
     3301 caggcttcaa agtcaccaag cagcacattc aatctgacgt caagctcctc ctgcgcgaca
     3361 actaccgcag cttcctggaa cgataggccc ggagaattct gttgtaattg ttacccggac
     3421 gtctcgttga tggtgcatcg tggttccaca agcctcgagc ttgtgcggta ccaggtaccg
     3481 atgaggagct aagtggcggg aactactgat gcctggtgaa aagtttacga gacagaggcc
     3541 gcgccgagag gccgcgacgg aatacccctt agcggctgat ctggctcgac cagcgggtcg
     3601 cgtggccatg
//
LOCUS       DBNGZC010000221         1936 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1877655, whole genome shotgun sequence.
ACCESSION   DBNGZC010000221 DBNGZC010000000
VERSION     DBNGZC010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1877655"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="AC16G0_04020"
     CDS             <1..395
                     /locus_tag="AC16G0_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HIR5098823.1"
                     /translation="WRDGGELLALTQVIVTARPGWRLDEVERLLTPEQRRVLGNPSFR
                     YLKIADIDPERVEHNLVPRQVLLVEVVSLDIASREIRQLVEEGRSIRHLVPDTVAAYM
                     AKNRLYQGAEPSRSQALSGPDIHGSVWR"
     gene            487..816
                     /gene="rsfS"
                     /locus_tag="AC16G0_04025"
     CDS             487..816
                     /gene="rsfS"
                     /locus_tag="AC16G0_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR5098824.1"
                     /translation="MVKIAAEAAAAVKPSYLAILDLRGLCTFADHFLIASAGSARQVR
                     AIADRVEEQLTRASIRMSHHEGRGEARWILLDYQDVVIHIFDDQTREFYDLERLWADA
                     PKQQLLG"
     gene            1179..1550
                     /locus_tag="AC16G0_04030"
     CDS             1179..1550
                     /locus_tag="AC16G0_04030"
                     /inference="COORDINATES: protein motif:HMM:NF044994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR5098825.1"
                     /translation="MTMSLKGPKLEKFKQRLLAKRRELLSEVRGSNVGSIETNTDGIQ
                     DIADQATSAYTKEFLLSIGDTERRMLKQVDEALHKIRQETYGLCESCGEMISERRLEA
                     LPFARLCITCQEEEERAKAGR"
BASE COUNT          356 a          579 c          686 g          315 t
ORIGIN      
        1 gctggcgaga cgggggggag ctcctggcct tgacccaagt gattgtgacc gcgcggcccg
       61 gctggcgcct ggatgaggtg gagcggctgc tcacgcccga gcagcggcgc gtcctgggga
      121 atcccagttt ccgctacctg aagatcgccg acatcgatcc cgagcgggtg gagcacaacc
      181 tggtcccgcg acaggttctc ctggtggagg tggtttccct ggacattgcc tctcgggaga
      241 tccggcagtt ggtggaggag gggcgaagca tccgacatct cgttccagac acggtggcgg
      301 cctacatggc caagaaccga ctgtatcagg gagccgagcc cagtcgctca caggccctga
      361 gcggcccgga catccacgga agcgtgtggc ggtagacgag agatccggcg cgacggccgt
      421 gaaggctgtg gccttcaatc gggcggccac acgacggacg gaggagcgga catcgattcg
      481 acggagatgg tgaagatcgc cgcagaggcg gcggcagcgg tcaagccgag ctatctggca
      541 attctggacc tgcgcgggct gtgcaccttc gcggaccact ttctcatcgc cagcgccggg
      601 tcggcccggc aggtgcgggc catcgccgac cgggtggagg agcagctcac gcgggccagt
      661 atccggatgt cgcaccatga ggggagggga gaggcgcgat ggattttgct tgactaccag
      721 gatgttgtca ttcatatttt cgacgatcaa acgcgtgaat tctacgattt ggagcggctc
      781 tgggcggatg cgccgaagca gcaattgctg gggtgatccg tcatcgagat cgggttcgac
      841 gacaggattg agcagaggct gaccgccgcg taaggcccgt caggccggac ggcgatccaa
      901 gttgacctcg cggaggcatg ctgggattcc agcttccgcg aggctttgta ttcccggccg
      961 tacgtcccgt cgggcgcttg ccgacgagac ggtgaccggc ggccgggagc gtgagggcag
     1021 gacccaagcc ggcaggttgg atgctggcgg ggagcatgaa aatcgggtgt ggagtccagc
     1081 gtgcaaggat ctggcgctga ttcaatcggg tgcgcgcaac cgaactgctg gcccttgggg
     1141 ggcgtcgccc agatccccag gccgcgggag gggaggcgat gacgatgtcg ctgaagggac
     1201 ccaagctgga aaaattcaag cagcgattgc tcgccaaacg aagagagctg ctgagcgagg
     1261 tccgaggttc caacgtcgga agcatcgaaa ccaacaccga cggaatccag gacatcgccg
     1321 accaggccac cagcgcctat accaaggagt tcctgctgtc catcggggat acggagcgcc
     1381 ggatgctcaa gcaggtggac gaggcgctgc acaagatccg gcaggagacc tacggcctgt
     1441 gcgagtcctg cggtgagatg atcagcgagc ggagactcga ggcgctgccc ttcgctcggc
     1501 tctgcatcac gtgtcaggaa gaggaggagc gggcaaaggc cgggcgctga caagctggag
     1561 tcgcctgtgt ccgcccgcgg catggacatc agcgcgggcg acagccgcct ggaccctgca
     1621 aggcgctgca tgcgcgggtt gaatttccag agaaatcccg ccctctcgtc tgcggatcgc
     1681 acgtcgccgg ccatcgggga cgcgctcatc ggatcgccat cccaatcccc atctccctgc
     1741 aaccgtcgct gcctccgtcc tccgccaggg gatggttcgc tctctcccaa gggtgtgcgc
     1801 gaccgactcc cacgagccgt tcaccgcagc ctccgactgt ggctaccggg gatagggctc
     1861 ttggctgcgc gtccggcgtg ggcccagtcg gccgaggggg ggcgactcct caccaacgat
     1921 gtccagggtt ggctgg
//
LOCUS       DBNGZC010000222         1804 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_116332, whole genome shotgun sequence.
ACCESSION   DBNGZC010000222 DBNGZC010000000
VERSION     DBNGZC010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_116332"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            58..912
                     /locus_tag="AC16G0_04035"
     CDS             58..912
                     /locus_tag="AC16G0_04035"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR5098826.1"
                     /translation="MPARYMVSSVLALSAIGVYGLRNSLVDVYVLIALGVIGYLFSKL
                     DIPLVTIALGIVLGNLTEQTFQQAVMVSMVDTGTAWLIFLKRPVAAILMLAALVVLGS
                     GILQAWREASIDQPAESRSASAPPKQRWLTLRGANILLSVGLIALSVYGLVAAREFSD
                     RGALLPRVICVSLIVLATIILVVNVNPRTGTPAGRLFPFSEIPWPLWWGVVVTFVLLG
                     LAADRFGFYESTFVFLAVTTWMMSAGEAGLQRRWLIPLAFAAGFDAALYLVFRLILEI
                     PTPPGLLL"
     gene            1052..>1804
                     /locus_tag="AC16G0_04040"
     CDS             1052..>1804
                     /locus_tag="AC16G0_04040"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5098827.1"
                     /translation="MALVVEGLVKRFGSVAAVDGVSFSIEPGEMLCLLGPSGCGKTTT
                     LRCVAGLETPDAGVLRLDGQLLADGTRGVLVPPHRRRFGMVFQSYAVWPHLSVFENVR
                     YPLRFGARMTREEMGQRTREALRLVRLEEHAERFPHQLSGGQQQRVALARALVMQPAA
                     LLFDEPLSNLDAKLREEMRGELADVQARLRVPALYVTHDQAEAMALATRIAVMHQGRI
                     RQEGTPGAIYRRPADAFVATVLGSTNLLPGVVE"
BASE COUNT          251 a          613 c          602 g          338 t
ORIGIN      
        1 tcacactcgc ctaccctgag aaaaaagtct gcgcagtcca gatagaaggc aggtgacgtg
       61 ccggcccgct acatggtgtc gagcgtcctg gcgctctccg ccatcggggt ctacggcctc
      121 aggaacagcc tcgtcgacgt gtacgtcctt atcgccctgg gtgtcatcgg ctacctgttc
      181 agcaagctgg acatccccct ggtcacaatt gccctgggga tcgtcctggg gaacctgacc
      241 gagcagacct tccagcaggc ggtgatggtg agcatggtgg acacaggaac cgcctggctc
      301 atcttcctga agcgccccgt agccgccatc ctcatgctgg cggccctggt tgtcctgggc
      361 tcgggcattc ttcaggcttg gcgagaagcc tccatcgatc agccggcgga gtcccggtcg
      421 gcatcggcac cgcccaagca gcgctggctg accctccgcg gcgccaacat cctgctctcc
      481 gtaggcctca tcgccctctc ggtatacggc ctggtggcag cccgggagtt ctccgaccgg
      541 ggcgccctgc tgccgcgcgt gatctgcgtg agtctcatcg tgctcgccac gatcatactt
      601 gtcgtcaatg tgaacccgag aaccggcacc cccgccggtc gtctcttccc cttctcggag
      661 atcccctggc cgctctggtg gggcgtggtg gtcacgttcg tgctcctcgg tctggcggcg
      721 gatcgcttcg gcttctacga atccaccttc gtcttcctgg cggtgacgac ctggatgatg
      781 tccgccggtg aggccggctt acagcgccgg tggctgatcc cgctcgcgtt cgcggcgggt
      841 tttgacgcgg ccctctatct cgtgtttcgc ttgatcctgg aaattcctac ccctcccgga
      901 ctgctgctgt gaggcaggat ccggtggcgt tcgcactgga gacgggtaga cgccactagc
      961 cacagggcgc accagggggg actcggactc cttgtgggtc ttgtgccttt tgtggcgaaa
     1021 tcgggttttg gtcggtagcg gagagcacct catggcgctg gtggtcgaag ggctggtcaa
     1081 gcggttcgga agcgtcgcgg ccgtggacgg cgtcagcttc agcatcgagc cgggcgagat
     1141 gctctgcctc ctgggaccca gcgggtgcgg caagaccacg accctgcgct gcgtggccgg
     1201 cctcgaaacg ccggacgccg gggtccttcg cctggacggg cagttgctgg ccgacggaac
     1261 ccgcggggtc ctggttccgc cccaccggcg ccggttcggg atggtcttcc agtcctacgc
     1321 ggtctggccg catctctcgg tcttcgagaa cgtccggtac cccctgcgct ttggcgcccg
     1381 catgacccgg gaggagatgg gacagcggac gcgggaggcc ctgcgcctcg tccggctgga
     1441 agagcacgcg gagcggttcc cccaccagct ctccggcggg cagcagcagc gcgtcgccct
     1501 ggcccgcgcc ctggttatgc agccggcggc tctcctgttc gacgagcccc tcagcaacct
     1561 ggacgccaaa ctccgcgagg agatgcgcgg cgagctggcc gacgtgcagg cccgtctgcg
     1621 ggtccccgct ctctacgtga cgcacgacca ggccgaggcc atggcgctgg ccacccggat
     1681 cgcggtgatg caccaggggc gcattcgaca ggagggcacg ccgggagcga tctaccgccg
     1741 gccggccgat gccttcgtgg cgaccgtcct cgggtcgacc aatctcctgc cgggggtggt
     1801 ggag
//
LOCUS       DBNGZC010000223         2748 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_605167, whole genome shotgun sequence.
ACCESSION   DBNGZC010000223 DBNGZC010000000
VERSION     DBNGZC010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_605167"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="AC16G0_04045"
     CDS             complement(<1..382)
                     /locus_tag="AC16G0_04045"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5098828.1"
                     /translation="MASATELAVWISESTQRALDLVADLSDEQMWGPRLPIVNPLHWE
                     MGHLAWFQEKWILRHVLGKPPIRSDGDSLYDSSAIAHDLRWDLPLPPRGETLAYLEAV
                     RDRILDCLRSGKLFDDLRYFALLST"
     gene            complement(523..1521)
                     /locus_tag="AC16G0_04050"
     CDS             complement(523..1521)
                     /locus_tag="AC16G0_04050"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009282525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR5098829.1"
                     /translation="MAFTGMTTNGGRIAVFYEHPEWFKPLFSELDRRDVAYDRLLAHE
                     HRFDPTEGRCPYDLIVNRMSPSAYTRGHTQAIFYTLQYMAHLKAIGANVLHGYDTYVY
                     EFSKARQIGLLHRLGLRHPRARVINHAAQAPAAAEGLVFPVILKPNIGGSGAKIVRFD
                     TPEALRVAALAGGMDLGIDGTALVQEYLPAEGNSIVRVEVLNGRFLYAIRLFLTADEF
                     NLCPADYCRLPANGARLDKAGLADGVSGRGVPVEGTTPPPQVIQDVLRITKGAQIEVG
                     GVEYLVNSRDGQVYYYDINALSNFVADAPRVVGFDPFPRLVDFLLQRAGLTSSVTV"
     gene            complement(1905..>2748)
                     /locus_tag="AC16G0_04055"
     CDS             complement(1905..>2748)
                     /locus_tag="AC16G0_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HIR5098830.1"
                     /translation="ACLSPGGFLLYRNVRRTEGLRELYKRVARTETGVEEAWVTLDEF
                     HKEFNELLFTVPADLFIPAGGRPETVDRSNWGRFLGPDGTPTARVIVEGANSFITPEA
                     RTLLQQKGIVVLRDASANKCGVISSSYEIIGNLLLTPKEFLARKEEYVRDVLAILEQR
                     AANEAELIFRRHREAGGKTTFTEISDAISVEINAQYATLFEFFQSRPELCSQPLYRRA
                     LFAHLPRMIRENPVFRLRIRNLPPKYRSAMLAVEIASVIVYRGGFERNFEEDLKAYLV
                     RMFP"
BASE COUNT          513 a          882 c          870 g          483 t
ORIGIN      
        1 aggtggagag gagcgcgaag taccgcaggt cgtcgaatag cttcccgctg cgcaggcaat
       61 ccaggatccg gtcacgcacc gcttcgagat acgccagggt ttccccgcgc gggggaaggg
      121 gcaggtccca gcgcaggtcg tgcgcgatgg cggaggagtc gtacagcgag tcgccgtcgg
      181 agcgaatcgg cggcttcccc agaacgtgtc gaaggatcca cttctcctgg aaccaggcga
      241 ggtgtcccat ctcccaatga agcggattca cgatgggcag gcgcggtccc cacatttgtt
      301 catcggaaag atcagccacc aggtccaggg cccgctgcgt ggactccgag atccagacgg
      361 ccagctctgt cgctgaagcc atgcgctccc ccctcaacca ccgacgccac aatcaggcta
      421 ttccaatacg gtctccctca ttacggcagc ctatcactcg ctcacacgac tttccctgcc
      481 tggcgcgcct tgatgcccgg actcccctgg acacccgggc ccctacacgg tcaccgagct
      541 ggtcaaaccg gcgcgttgca gcagaaaatc caccagacgg gggaatgggt cgaagccgac
      601 cactctcggg gcatcggcca cgaagttcga cagggcgttg atatcgtagt aatagacctg
      661 tccgtcgcga ctgttcacca ggtactccac cccccccacc tcgatctgcg cccctttcgt
      721 gatacgcagg acatcctgga tgacttgagg cggcggggtg gtgccctcca ccggcacgcc
      781 ccgccccgag acgccgtcgg cgaggcccgc cttgtcaagt cgcgccccgt tggccgggag
      841 ccggcaatag tccgccggac agaggttgaa ctcgtcggcc gtcaagaaga ggcggatcgc
      901 atacagaaac cgcccgttca ggacctcgac gcgcacgatg ctgttcccct cggcggggag
      961 gtactcctgc accagcgcgg tgccgtcgat gcccaggtcc atccccccgg ccagggccgc
     1021 cacccgcaga gcctcggggg tgtcgaaacg gacgatcttc gccccgcttc ccccgatgtt
     1081 tggcttcaga atgaccggaa agacgagccc ctcggcggcc gccggcgcct gtgctgcgtg
     1141 attgatgacg cgggcgcgcg gatgccgcag gcccaggcga tgcagcaggc cgatctgccg
     1201 ggccttggag aactcgtaga cgtacgtgtc gtagccgtgg aggacgttgg cgccgatagc
     1261 cttcaggtga gccatatact gcaaggtgta aaagatcgcc tgggtgtgac cccgggtata
     1321 ggctgacggg ctcatccggt tgacgatcag atcatatgga catcggccct cggtcgggtc
     1381 aaatcgatgt tcgtgggcca aaagtcgatc gtaggcaacg tctcgacgat ccagctcgct
     1441 gaagagcggc ttgaaccact cggggtgttc ataaaagacc gcaatgcgcc ctccgttcgt
     1501 cgtcatgccg gtgaatgcca tgcctgactc ctgccgcgcg tcctctcagg gcgcgtcgat
     1561 tcaaaacgtt ctgcccgtcc caggacctct ccttccgatt cacggctgtc tactctaact
     1621 tcctaagaag gaaccgacaa ctatttgcct gaagaacggg cgcagccggt gtcggggatc
     1681 cctcggggca cgtggggtgc cggatcaggc ttgggtggcg agtaggcgag agaaggcgca
     1741 ggaggttggg tggtcgatca agcctggcta ctggattgct gctgaagtca tgaggggtcg
     1801 cctcctgcga gagggcaccg gacagtgcct tcagggggcg atgagcaatc gccgcgtctc
     1861 atccgtggaa agatgtcccg tcccctccgg cgcaggggtg acggtcaggg gaacatccgg
     1921 accaggtacg ctttcagatc ctcctcgaag tttctctcga acccaccccg gtagacgatc
     1981 accgatgcga tctcgacggc cagcatggcc gaccggtact tcggcggaag attccggatc
     2041 cgaaggcgaa ataccgggtt ctcccggatc atccgcggga ggtgggcgaa gagggcacgg
     2101 cggtaaaggg gctggctgca cagctcgggc ctcgactgga agaattcgaa tagggtcgca
     2161 tactgggcgt tgatctccac gctgatggca tcggaaatct cggtgaaggt cgtcttcccg
     2221 ccggcctcgc ggtgccgccg gaagatcagc tccgcctcgt tggcggcccg ctgctcgagg
     2281 attgcgagca cgtcccggac gtactcctcc ttgcgagcga gaaactcctt cggggtcagg
     2341 agcaggttgc cgatgatctc gtacgacgaa gaaatgacgc cgcacttgtt ggccgacgcg
     2401 tcccgcagga ccacgatccc cttctgctgc aacagggtcc tggcctcggg ggtgatgaag
     2461 gagttggccc cctccacgat cacccgcgcc gtgggcgtgc cgtccgggcc caagaaccgc
     2521 ccccagttcg accgatccac ggtctcgggc cgtcccccgg cggggatgaa caggtcggcg
     2581 ggaaccgtaa aaagaagctc gttgaattcc ttgtggaatt catccagggt gacccacgcc
     2641 tcctccacac cggtctccgt ccgcgcgaca cgtttgtaga gttcccgcag tccctctgtc
     2701 cggcggacgt ttcgatacag gaggaagcca ccgggggaaa ggcaggcc
//
LOCUS       DBNGZC010000224         3762 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_507446, whole genome shotgun sequence.
ACCESSION   DBNGZC010000224 DBNGZC010000000
VERSION     DBNGZC010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_507446"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            598..1380
                     /locus_tag="AC16G0_04060"
     CDS             598..1380
                     /locus_tag="AC16G0_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HIR5098831.1"
                     /translation="MWTIYPLDVGDLEHDKSEAIFRRGMGERGTERFVAWYLTDGTSR
                     VLVDTGPPDEAHSRKWHPYNNPRISERQQIGSALAQHGVSLDQISLVILTHLHWDHAG
                     NMPLFRNADFIVSHEELVYAVDPCPIHYVAYEAPQLGLTPPFLPVMPRLRTISLREQE
                     VAPGLVALPTPGHTPGSISLVVTTTAGPYVIAGDAVSCYENLEGDPAKKLKYLPTGIF
                     TDLLATWNSMALIEAKAQFRRDHILPSHDSRVFRHRSYPPAA"
     gene            1470..2351
                     /locus_tag="AC16G0_04065"
     CDS             1470..2351
                     /locus_tag="AC16G0_04065"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5098832.1"
                     /translation="MTVELIVRAILSGILMGAIYGVLGMGFTIVYGIVDLPLFTHGVF
                     ALWAMYLLWMLVKHTGIGPYPGFLLLLGMAYIFGILLYKAVLRYILDIEHEMQLVFCL
                     GLLIALSNLALIIFGPTSLLLGIDWLERVVFLGEVSLKTAMIVGATVSVLFIVCMELF
                     FRKTETGKAIRACADNRRGALIIGLNVNKMYMTAIGISLVCSAVAGLAFAPLVPIYPD
                     LGFEYAVLACLVAVLGGAGDMRGALVAGFVMGLLLSIQQMFYDLTLSRLILYAIMLGL
                     LMFRPTGILGRGKSFAK"
     gene            2383..3375
                     /locus_tag="AC16G0_04070"
     CDS             2383..3375
                     /locus_tag="AC16G0_04070"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5098833.1"
                     /translation="MKSTEINGGRLQWIIPGIVLVALLAVPYCVGSYLTQVVLFGLVT
                     AYLCGAWNISGGYGGLVSFGHAAFIGIGAYATAIPLVYYGLPGWLGMLLGGGISAAFG
                     FVLTWTICRFHVRGFYFAVSTILFAEILMSIAARSRYLGRGMGVPLPPVENVFNLQFL
                     GIIPYYYMALVLACGMVVITQQIERSRLGWYLRATRQSEDASAALGIDVTMVKVVAMT
                     LSAFLTGVGGGVYVLALRYCSPYDVFGLMFSTTLMLGTLLGGRGTVYGPIIGIAMLTA
                     VKEILTFTGEALGGISSFALVLVGWGVILCLVAKYLPNGIGPWIGNRVVRSNAR"
     gene            3424..>3762
                     /locus_tag="AC16G0_04075"
     CDS             3424..>3762
                     /locus_tag="AC16G0_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5098834.1"
                     /translation="MALLEIHNVRKTFGGLVALDGMNIVVEEGRIYGLIGPNGAGKTT
                     LFNIASGFYRPDSGDIVFGGERVTDLGSHERCARGLARTFQIPKMFPDMSVVDNVLIG
                     ALSRTKTVKTA"
BASE COUNT          617 a         1137 c         1228 g          780 t
ORIGIN      
        1 ccgagcgata ccctggagac cctggacctc cccttcctca cccaggtgac caccgccacc
       61 gcagccacgg ccgcccatct ggtactacct gatttgtcct gattccgctt gacatcccca
      121 cccgccgctg gtagcgtccc cgctcgcggc gcttcgcccc tggttgttgc tccccggcgc
      181 ggcatggctc cggtgtggcc caccaggcat ggtgaccacg cggggccgcg cccggtttcc
      241 acgtgcctat cccctggggc aatactgcct ggtgtccggc gaggtgcccg gcactgaggg
      301 ccgtggtctc cagagcatcc cgacctatcg atcgtttccc ttcggcgacg acatcgacag
      361 cgtgatcacc gccaacaccg agaatatgtg ccgttcgctc tgcgcggtgc tgaagcttcc
      421 cgcagtcatg tgtggatccc cgcgtggacc aagactgata ccggaacgag gaggcgctgc
      481 ggaatgaggg ggaggctccc ttccaccagt gatcttctgc ggagtggctc gaaccgtcgc
      541 cgggatggcc cgagtcgagc cgtgcgtcgt tagccgatgc cgaaggaggc gcctgccgtg
      601 tggacgatct atcccttgga cgtgggcgac ttggagcacg acaagagtga ggcgattttc
      661 cgacgcggga tgggcgagcg gggcacggag cggttcgtcg cctggtacct gaccgacggg
      721 acgtcgcgcg tcttggtcga cacgggtccg cccgacgagg cgcactcgcg gaagtggcat
      781 ccctataaca atccgagaat ctcggagcgg cagcagatcg gcagcgcgct ggcccagcac
      841 ggggtgagtc tcgatcagat tagtctggtg atcctcacgc accttcactg ggaccacgcg
      901 gggaacatgc ccctctttcg gaacgccgac ttcatcgtgt cccatgagga gctagtctac
      961 gccgtggatc cctgcccgat ccattacgtc gcgtacgagg cgccccagct cggactgacg
     1021 ccgccgtttc tgcccgtcat gcccaggctg cggaccatca gcctgcggga gcaagaggtc
     1081 gcgccggggc tggtcgcgtt gccgacgcct ggtcacacac ccgggtccat ctcgctggtg
     1141 gtgacgacga cggcggggcc gtatgtcatc gcgggagatg ccgtctcctg ttacgagaat
     1201 ctggagggcg atcccgccaa gaagctgaag tacctgccga cggggatctt caccgatctc
     1261 ctggcgacct ggaacagcat ggccctcatc gaggcgaagg cacagttccg gcgggatcat
     1321 atcctgccct cgcacgattc gagggtcttc cggcataggt cgtatccccc ggccgcttag
     1381 aaggggtcga aggaggcggg cgacgctctc cgggggagcc ggagggggcg tgccaggtcc
     1441 tttcggacgc gagggccaag gatgaggcga tgactgtcga gctgatcgtc cgggcaattc
     1501 tgagcggcat cctgatgggc gcgatctacg gcgtgctggg gatgggtttt accatcgtct
     1561 acggcatcgt ggatctgccg ctctttaccc acggcgtgtt cgcgctgtgg gccatgtacc
     1621 tcctttggat gctcgtgaag cacacgggca tcggccccta ccccggcttc ctgctgctcc
     1681 tcgggatggc ttatatcttc ggcatcctgc tctacaaggc ggtgctgcga tacatcctgg
     1741 acatcgagca cgagatgcaa ctggtgttct gcctgggcct cttgatcgcc ttatccaacc
     1801 tggcgctcat catttttggt ccgacatcct tgctgctcgg cattgactgg ctggaacgag
     1861 tggtgtttct gggcgaggta tccctgaaga cggcaatgat agtcggcgcc accgtgtccg
     1921 tgctgttcat cgtctgcatg gagctgttct ttcgcaagac ggagacggga aaagcgattc
     1981 gcgcatgcgc cgacaacaga cgtggggctc taatcatcgg cttgaacgtg aacaagatgt
     2041 atatgaccgc catcgggatc agcctggtgt gctcggcggt cgccggcctc gcctttgcgc
     2101 cgctggtccc catttacccg gatctcggct ttgagtatgc ggtccttgcc tgtctggtcg
     2161 cagtgctggg gggcgcagga gatatgaggg gggctctggt cgccggcttc gtcatgggcc
     2221 tgctgctcag cattcagcag atgttttatg acctcacgct gtcgcgactc atcctgtacg
     2281 caatcatgct tggcctgctc atgttccggc ccaccggcat cctgggccgg ggcaaatcgt
     2341 ttgccaagta aggcacggcg aaagtagaag atggtgactc aaatgaaatc gaccgagatc
     2401 aacggtggca ggctccagtg gatcatccca ggcatcgtgt tggtggcgct gctggcagtc
     2461 ccctattgtg tcggtagcta cttgacccag gtcgtgctgt tcggacttgt cacggcgtat
     2521 ctctgcgggg cgtggaatat ctccgggggt tacggcggct tggtgtcttt cggtcacgcg
     2581 gctttcatcg ggatcggggc gtatgcgacg gccattcccc tcgtgtatta cggactgccc
     2641 ggttggctgg gaatgctcct gggcggcggg atctccgccg cttttggctt cgtcttgacc
     2701 tggacaatct gccgctttca tgtcaggggg ttctatttcg ccgtcagcac catacttttc
     2761 gccgagatcc tcatgagcat tgccgcgcgc tcacggtacc tgggacgagg catgggggtg
     2821 ccgctgcccc cggtggagaa tgtcttcaat ctccaattcc tgggcatcat tccctattac
     2881 tacatggcgc tcgtgctggc atgcggcatg gtcgtcatca cgcagcagat agagcgatcc
     2941 aggctgggat ggtatctccg ggcgacgcga cagagcgagg acgcttcggc ggccctgggc
     3001 attgacgtca cgatggtgaa ggtggtggcc atgacgctca gtgcgttcct gacgggcgtc
     3061 ggcggcgggg tgtacgtgct ggccctgcga tactgcagcc catacgacgt cttcggcctg
     3121 atgttcagca cgaccttgat gctgggcacc ctcctgggcg gcagaggcac cgtgtacgga
     3181 ccgatcatcg ggatcgccat gctcaccgcc gtgaaggaga tcttgacctt caccggcgag
     3241 gcgctcggcg gcatcagcag tttcgccctg gtgctggtcg gctggggcgt cattctgtgc
     3301 ctcgtcgcca agtacctgcc aaacggcatc gggccctgga tcgggaatcg agtcgtgaga
     3361 agcaacgcgc ggtgagcggg ccgcggttca ggttgaaccc ggcggagcgg gtgagacggt
     3421 cgcatggcgc tgcttgaaat tcacaatgtg cgaaagacct tcggtgggct ggtcgctctc
     3481 gacgggatga acatcgtggt cgaggaaggc aggatctacg ggctgatcgg cccgaatggt
     3541 gcggggaaga cgaccctgtt caatattgcc tcgggcttct accggccaga ctccggtgac
     3601 atcgtctttg gcggcgagcg cgtgacggac ctcggcagtc atgagcgctg cgccaggggg
     3661 ctggcccgga cgttccagat ccccaagatg tttccagaca tgtcggtggt ggacaacgtc
     3721 ctgatcggcg cgctcagccg gacgaaaacc gtgaagacgg cg
//
LOCUS       DBNGZC010000225         7080 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2268760, whole genome shotgun sequence.
ACCESSION   DBNGZC010000225 DBNGZC010000000
VERSION     DBNGZC010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7080)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7080
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2268760"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..991
                     /locus_tag="AC16G0_04080"
     CDS             329..991
                     /locus_tag="AC16G0_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020290049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR5098835.1"
                     /translation="MSYHLNFGYVWTHFDKLSWGLLLSLELAFVSILIGMVIGLSLAL
                     LYTAGGKLIRGVVAAYVEFIRNVPLLLLVYLVFYGVPSVGGFAYDATTSFIITLSVYA
                     GAYMVEVFRAGLAAIPKGLTDAGKAIGLTGWQRLLSVRLPTMFRIILPSLSNAYVSLF
                     KDTSLASVIAVPELTYGAQWIKTNSFRVIETWAVVTPMYLVTGYALLFALQRLERRFA
                     IH"
     gene            991..1638
                     /locus_tag="AC16G0_04085"
     CDS             991..1638
                     /locus_tag="AC16G0_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR5098836.1"
                     /translation="MDFAIQSLPFLWQGLLVTLHVSALIVFFALLAGTVMGIGIVYGP
                     RWLYWPIRLYSDFFRGIPLLVLIFFMYYGLPIFMVNLSNFAAVVLALSAFKTAHVIEV
                     VRGAIQSIPRGQTEAAKSIGLTFSQRLLYVIAPQAVRRFLPPWINAVVDTVKGSALVS
                     LVGIVDLMLAIQQVIGRTYRPMPLYVLGAIMYFIINYTLSLASRKLEARFAYVRE"
     gene            1642..2436
                     /locus_tag="AC16G0_04090"
     CDS             1642..2436
                     /locus_tag="AC16G0_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR5098837.1"
                     /translation="MSDTHTAGPVVRVRGLKKSFGANRVLDGIDLEVRKGEVVVVIGP
                     SGSGKTTLLRCINLLETYDEGSVQVDGVEVGYREAPGGARHRRGDRELSKIRADIGMV
                     FQLFNLFPHLTAQQNVMLGLRKVRGLPAAEAKQVAARWLTRVGLGDKLDSLPSQLSGG
                     QQQRVGIARAVAMDPKVLLLDEITSALDPELVGEVLNVVQELARDGMTMIVVTHEMTF
                     ARDVATRIVFMDGGRIQVEGTPRQVLAKTDHERLKSFLARIEMKHH"
     gene            2548..3762
                     /locus_tag="AC16G0_04095"
     CDS             2548..3762
                     /locus_tag="AC16G0_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020011820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR5098838.1"
                     /translation="MSAETTSRSNIRERLGLRPIINVSGTMTALGASIVVPEAVQAVS
                     QILPEFVEINDLQRKTSSVIARLTGAEAGFITACASSGITLAVAGCMTGTDLGRIEQL
                     PDTTGMKDEVVIQLGHMVGYGAPVEQAIRLAGAKAVPVGQATEVREYQLAHRLTERTA
                     AAVYVVSHHTVQYGQLPLDRFAQICHAKRVPVVADLASEYDLRGFLAAGADIAIYSAH
                     KFLGGPTAGIVAGPKALVRAAFLQNFGIGRGMKVGKEGMVGAMAALQAWEKRDHAAIR
                     IKETDHLTLWRKRFEGLPGVTVSIEPDPTRNPLDRLKLALDPERARITAWELADVLAG
                     GHPPIIVRDHEAEHGFFTMDPCNLHPGEAEVVADRVVRELERARTRSRGQEYSATERK
                     ARRFERLLAWPD"
     gene            3850..4356
                     /locus_tag="AC16G0_04100"
     CDS             3850..4356
                     /locus_tag="AC16G0_04100"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR5098839.1"
                     /translation="MSVKPGWQGRVYEDFEVGDVYHHPLGRTITQTDNIWFTLLTVNA
                     NPIHFDSVYASQTEFKRPLVDSTFTLALITGLSVSDVSQNAVNLGWEEVRLPHPVFEG
                     DTLYAESLVLEKRDSRSRPHMGIVSLKTTGLNQRGEVVIEFKRSILVYRRGHVPHIQR
                     PTLPDPPR"
     gene            4810..5244
                     /locus_tag="AC16G0_04105"
     CDS             4810..5244
                     /locus_tag="AC16G0_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098840.1"
                     /translation="MRPLPGYRFAFPPGAPLTYSDPDSGTTYCLSQPTGFYYECGYSS
                     SAPYPVGPFPPMPPISGPPFAEQTAPPASGVLMFRLPQGSEVAVDGVPIGLSDGLGII
                     SVTPGRHKLVLRVGGKETEHTVSVGTHRILTITPTSIVATEP"
     gene            5305..6402
                     /locus_tag="AC16G0_04110"
     CDS             5305..6402
                     /locus_tag="AC16G0_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013539.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR5098841.1"
                     /translation="MPVLERRAAHPFHMYDAILAQPSCVAEALEANARQLDGAAELFR
                     QHEHLILCGIGTSFHAALVGEHLMRLIAGDQPFTQAVHSFDFVLYPPPIRPGTGVLVV
                     SHRGTKNYSLQALFRANEHKAPSVTITGKDSAEGIRKARIVMVTTDQEVSSAHTKSYT
                     ASLSLLAGLAIRMGGRVGRDTAGAEAQLAQIPALMQRAFQTEAQVQAIARTLAEKEFI
                     VFIGGGPNAATAYEVALKMKETNYTPCEGFQVEQFLHGPLAGLSQRMAVWVIAPPGPS
                     YGRCLNVVQAANAIGATTIALTEEGDPALARTAVHAIGLPALAEALTPLIYVIPLQLF
                     CYQLALRKGANPDTFHLDDPRHQEAKRHYNL"
BASE COUNT         1183 a         2388 c         2223 g         1286 t
ORIGIN      
        1 tacggcgcgg gcgtgcgcca gggcgacctc gactggctgc actgggtcga cacgtgcctc
       61 aacgtggcga tgttcggcca ccagaccgag gtctatgaca aagccttcga ggaattcttc
      121 ggccagaagc cgccggcccg aaaaccggga ttcccgccgt tctagctcgg gtgcacttgc
      181 gcgagttcct gattgcgcct gcctgcctct cacccccgcc ctttcccacg ggagggaggg
      241 cgggtgaggg ggggccggtc ggcgcatgcc gatcctcctc gcgtccgcct gaggccgcgg
      301 acctctccga tgtgatggga aatcgacggt gagctatcac ctcaacttcg gctacgtctg
      361 gactcacttc gacaagctgt cctggggact cttgctgagc ctcgagctgg ccttcgtctc
      421 gatcctcatc gggatggtca tcggactcag cttggccctg ctctacaccg cggggggaaa
      481 gctcatccgc ggcgtggtgg cggcctacgt cgagttcatc cgcaatgtcc cgctcctcct
      541 tctggtgtac ctcgtcttct acggggtgcc gagcgtgggc ggcttcgcct atgacgcgac
      601 caccagcttc atcatcaccc tctcggtcta tgccggggcg tacatggtcg aggtcttccg
      661 ggccgggctg gccgcgatcc ccaaagggct cacggatgcc gggaaggcca ttggcctcac
      721 cggatggcag cgactcctct ccgtccggct gccgaccatg ttccggatca tattaccgtc
      781 gctcagcaac gcctacgtct cgctgttcaa ggacacttcc ctggcctcgg tcatcgcggt
      841 accggagctg acgtacggcg cgcagtggat caagaccaac tcctttcggg tgatcgagac
      901 ctgggcggtg gtcacgccca tgtatctcgt caccggctac gccctcctgt tcgccctcca
      961 gcggctggag cggcgcttcg cgatacactg atggacttcg ctatccagag ccttcccttt
     1021 ctgtggcagg gcctcctggt caccctccac gtctcggcgc tcatcgtctt cttcgcgctc
     1081 ctggccggga cggtcatggg gatcggcatt gtgtacggcc cccgctggct ctactggccc
     1141 atccggctgt acagcgactt cttccggggg atcccgctcc tggtcctgat cttcttcatg
     1201 tactacgggc tgccgatctt catggtcaat ctcagcaact tcgccgcggt cgtcctggcc
     1261 ctctccgcgt tcaagacggc acacgtcatc gaggtcgtgc gcggcgccat ccagtccatc
     1321 ccgcggggtc agaccgaggc tgccaagtcc atcggcctca ccttcagcca gcgcctcctc
     1381 tacgtgatcg ccccccaggc ggttcgtcgg ttcctgccgc cctggatcaa cgccgtggtg
     1441 gacaccgtga agggcagcgc cctggtctcc ctcgtgggga tcgtggacct gatgctggcg
     1501 attcagcagg tgatcggccg gacctaccgg cccatgccgc tctacgtcct cggcgccatc
     1561 atgtatttca tcatcaacta caccctctca ctggcgagcc gcaaactcga ggcgcgcttc
     1621 gcctacgtcc gggagtgatc cgtgtccgac acccataccg ccggtccggt cgtccgcgtc
     1681 cgcggcctca agaagtcctt cggcgccaat cgcgtgctcg acgggatcga cctcgaggtc
     1741 cggaagggcg aggtcgtggt ggtgatcggc ccttcgggga gcggcaagac cacgcttctc
     1801 cgctgcatca acctgctgga gacctacgac gagggctcgg tgcaggtgga cggcgtggag
     1861 gtggggtacc gcgaagcacc cgggggggca cgccaccgcc gcggggatcg agagctttcg
     1921 aagatccgcg ccgacatcgg catggtcttc cagctcttca acctcttccc acacctcacg
     1981 gcacagcaga acgtgatgct gggcctgcgc aaggtgcgcg ggctcccggc ggccgaggcg
     2041 aagcaggtgg ccgcccgctg gcttacgcgc gtgggactgg gggacaagct ggacagcctg
     2101 cccagccagc tttccggcgg gcagcaacag cgcgtgggga tcgcccgcgc cgtggccatg
     2161 gatcccaagg tgctcctcct ggacgagatc acgtcggccc tggacccgga actggtcggc
     2221 gaggtcctga acgtggtgca ggagctggcg cgggacggga tgaccatgat cgtggtcacc
     2281 cacgagatga cctttgcccg cgacgttgcg acgcgcatcg tcttcatgga cggcggccgt
     2341 attcaggtcg agggcacccc gcgccaggtc ctggccaaga ccgatcatga gcggctgaag
     2401 tcgttcctgg ctcggatcga gatgaagcat cactgagccg gattacccgc ggatcccttc
     2461 acggatgatc cggaccaatg accacagggg gttccgcatt cgcttttcca atgatccgga
     2521 tgaaccagcc gaagaggagt ccgagccatg tctgccgaga ccacttcccg atccaacatc
     2581 cgcgagcgtc tggggctgcg cccgatcatc aacgtgtccg gtaccatgac ggccctgggg
     2641 gcctccatcg tggtgcccga ggcggtgcag gccgtctccc agattctccc cgagttcgtc
     2701 gagatcaacg acctccagcg caagaccagc tccgtgatcg ccagactcac gggcgcggag
     2761 gccgggttca tcaccgcctg cgcctcctcc gggatcacgc tggccgtggc gggctgcatg
     2821 accggtaccg acctcgggcg gatcgaacag ctccccgaca ccaccgggat gaaggatgag
     2881 gtcgtcatcc agctcggcca catggtggga tacggggcgc cggtggaaca ggccatccgc
     2941 ctggccggcg ccaaggccgt tcctgtgggg caggccaccg aggtgcggga ataccagttg
     3001 gcgcaccgat tgaccgagcg gacggcggcg gccgtctacg tcgtctcgca ccacaccgtg
     3061 caatacggcc agctcccgct cgaccggttc gcgcagatct gccacgccaa gcgggtgccg
     3121 gtggtcgcgg atctcgcctc cgagtacgat ctgcggggct ttctcgccgc cggcgcggac
     3181 atcgccatct acagcgccca caagttcctg ggtgggccga ccgccgggat cgtcgccggc
     3241 cccaaggccc tcgtgcgcgc ggcattcctg cagaatttcg gcatcggccg gggtatgaag
     3301 gtgggcaagg aggggatggt cggcgccatg gcggcgctcc aggcctggga gaagcgggac
     3361 cacgccgcca tccgaatcaa ggagaccgat catctgacgc tctggcgcaa gcgcttcgag
     3421 gggctgccgg gcgtgacggt ctccatcgag cccgacccca cgcggaaccc tctggaccgc
     3481 ctcaagctcg ccctggatcc ggagcgcgcc cgcatcaccg cctgggaact ggccgacgtt
     3541 ttggccggag ggcatccgcc catcatcgtg cgggaccacg aggccgagca cgggtttttc
     3601 accatggacc cgtgcaatct ccatcccggc gaggcggagg tggtggctga tcgcgtcgtg
     3661 cgggagctgg agcgagcgcg cacccgcagc cggggccagg agtattccgc caccgaacgc
     3721 aaggcgcggc ggttcgagcg cctgctggcc tggccggact gacctcgggc ctccgctctg
     3781 gctctcaggg tccggaaggg ctgttccaaa gggcggattg gggcgctgaa gagaaaggga
     3841 ggccgatcga tgagcgtgaa accgggctgg cagggacgcg tctacgagga cttcgaagtc
     3901 ggcgatgtgt accaccaccc cctcgggcga accatcaccc aaaccgacaa catctggttc
     3961 accctgctca ccgtgaacgc caacccgatc cacttcgaca gcgtgtacgc gtcgcagacg
     4021 gagttcaagc ggccgctggt cgattccacc tttaccctcg ccctcatcac cggcctgtcg
     4081 gtctccgatg tgtcgcagaa cgcggtcaac cttgggtggg aagaggtgcg cttgccccat
     4141 cctgtgttcg agggcgacac gctctacgcg gaaagcctcg tgctggagaa gcgggattcg
     4201 cgctcacgcc cgcacatggg catcgtaagc ctgaagacca ccgggctcaa tcagcgcggc
     4261 gaggtcgtca tcgaattcaa gcggtcgatc ctggtgtaca ggcgcgggca cgtgccgcac
     4321 atccagcgtc cgaccttgcc cgatccgccc agatgaattg acgccccgga aagcaccctg
     4381 gatcatgcgt ggacgatcac actgggcatc ggcggtccgg gtttgacgcc caggtggtct
     4441 tgccgggaag gattccgaag tgctccgggc tttgttctgc cccgaacttt ttctccttga
     4501 cgaagcgagc gattgggacc atcatccgta caacgtaggt cttaattaca atatgggggg
     4561 gccgcaatga ttgagtgggt ctcccggcaa gaaggtaaag tcctctccac gaagcgaggg
     4621 accgtgggtc gcggaacggc gcacagcccc gccagaagca tcgtatcgtt cgtgatactt
     4681 gcggtggccc tcagcgtgtt gtcctcccct caaccagcta ctgcgtggcg gcatgggcac
     4741 gtgttcgtca gcccgggcgc ctatgtcgga tacccgtatg cctcttcccc gtcggtcttg
     4801 ttcccggggg tgaggcctct gcctgggtat cgcttcgcct ttcctcccgg tgctcccctg
     4861 acgtactccg atcctgacag cgggacgacc tactgtctgt cccagcccac aggcttctat
     4921 tacgagtgtg gctattcctc ctccgccccc tatccggtcg ggccgtttcc ccccatgcct
     4981 ccaatctccg gccccccctt cgcggagcag acggcacctc cggcttcggg cgtcctcatg
     5041 ttcagattgc cgcagggttc cgaggtggcg gtggacggcg tgcccatcgg tctgagcgat
     5101 ggtctcggga tcatctccgt gacaccgggc agacacaagc tggtcctgcg ggtgggcggg
     5161 aaggaaaccg aacacacggt cagcgtcggc acgcatagga tcctcaccat cacccccaca
     5221 tccatcgtcg caaccgagcc ctgaccctaa cggaccgcgg atctgccacc tcgtcgcacc
     5281 gtccacccga attgggatgc aagcatgccg gtacttgaga gacgggcggc acacccgttt
     5341 cacatgtacg atgcgatcct ggcgcagcca tcgtgtgttg ccgaggcgct ggaggccaac
     5401 gcccgtcagc ttgacggggc cgccgagctg ttccgacagc acgagcatct gatcctctgc
     5461 ggtatcggca cctcgttcca cgcggcgctg gtcggcgaac atctcatgcg actcatcgcg
     5521 ggagaccagc ccttcaccca ggcggtccac tcttttgact tcgtgcttta tcccccgccc
     5581 atccgccccg gcacaggagt cctggtcgtg agtcaccgcg gcaccaagaa ctactccctg
     5641 caggccctgt tccgggccaa cgagcacaag gcgcccagcg taacgatcac cgggaaggac
     5701 tcggcggagg gtatccgcaa ggctcgcatc gtcatggtca ccaccgacca ggaagtctcc
     5761 tcggcgcaca ccaagagcta caccgccagc ctgtcattgc tggctggcct cgcgatccgg
     5821 atgggtggca gagtggggag ggatacggcg ggcgccgaag cccagttggc gcagattcct
     5881 gccctgatgc aacgcgcctt ccagacggag gcccaggtgc aggcgatcgc ccggacgctc
     5941 gccgagaagg aatttattgt cttcatcggg ggtgggccga atgcggcaac ggcctacgag
     6001 gttgccctga agatgaagga gacgaattac acaccctgcg agggcttcca ggtcgagcag
     6061 tttctccacg gccccctggc tggcctgagc cagcggatgg ccgtctgggt tatcgccccg
     6121 ccgggaccga gctacggacg ctgcctgaac gtggtacagg ccgcaaatgc catcggcgcc
     6181 acgaccatcg ccctgaccga agagggcgat ccggctctgg ccaggaccgc cgtccacgcc
     6241 attgggctgc cggcgctagc cgaggccctg acacccttga tctatgtgat cccgctccaa
     6301 ctcttctgct atcaactggc gctccgcaag ggggccaacc cggacacctt ccacctggac
     6361 gatccccgcc accaggaagc caagaggcat tacaacctct gaggcaaccc cccgattcat
     6421 ccgtgttgac agcttcgtca tggcaggtta ggttcgccta ccatgacggt tgcccttagg
     6481 tcggtggagg ggggagggca atgtcagcgg tcgtcgttcc gtccgcgcac ggcgagcggg
     6541 ttttggctta tagagcgcgt tcacgcggcc cttcggggag cagtgagccc tgaagggccg
     6601 ttctgtttcg aggctggccc cggccagcaa tgacgcgctt ccggcgtggt cgggcgatcc
     6661 agggggatat cgcctacccg gggagccgat ccgcggtcct ctcggaggtg attgcagcgt
     6721 ccgcgcgtgg aatcagaggg cggcagcccc acccgccaaa ggatgtttcg ctgattgtcc
     6781 tgtcgtgacc gttgatccgg cggagcggtc aacgccttcg tgatcgcaca cccactcctg
     6841 tacgatcgcg gtctcccctt cgcatccccc gaaaccgaag ggcatgcgag gatactcata
     6901 ccaagagcac ggcaagagga ggatatccca tgcgacgaat agcgtgtgcc atagggttga
     6961 tcactctggt tgccctctcc ttttctcccg tatgggcggc cccactcgtc caggtctcca
     7021 aagcgccaag tccattcgcc agttgcacgg cagacaacgc ggcgggacag ccgggcacga
//
LOCUS       DBNGZC010000226         2083 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_997630, whole genome shotgun sequence.
ACCESSION   DBNGZC010000226 DBNGZC010000000
VERSION     DBNGZC010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_997630"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="AC16G0_04115"
     CDS             <1..296
                     /locus_tag="AC16G0_04115"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:NF016084.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIR5098842.1"
                     /translation="ARGEGLDVVGPVPPDSFFPLALRGDFRGMVFMYHDQANIGRKIL
                     GRDQPGVSLFLGMPVPVGTVPHGTAYDIAWKGIAKHAMIVRAFSVTAALAASK"
     gene            341..1333
                     /locus_tag="AC16G0_04120"
     CDS             341..1333
                     /locus_tag="AC16G0_04120"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR5098843.1"
                     /translation="MRRHTILWMMIALLLVGIAGPGAVGAQQPVLIKLGNVQATGDIV
                     QTGLKKFADLVSERTKGRVQVQIFPASQLGTEQELLEGVQLGTVHMFEGSTGSVGRFL
                     PDLEAFAAPYIWRDTDHMLKVVRGPIGQELADRLVKAKGMRVLDFGWLFGNRYLTTKS
                     KPIHKPEDLKGMKIRVQPTAIYLETIKAMGANPTTMDFKEVYLGLQSGVIDGQENPPF
                     VIFNNKLFEVQKYVMLTAHITQNQAIVINDKFYQGLSPDFQKILQDAAHEAGNFQNEL
                     ILKSEQEYLDKLKEKGMIIVQPDVKAFREATKDVWKKVSEKWEPGLYEKIQAVK"
     gene            1354..1845
                     /locus_tag="AC16G0_04125"
     CDS             1354..1845
                     /locus_tag="AC16G0_04125"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR5098844.1"
                     /translation="MKPTVVDRLVNWGLAACLGTMTGVVFVSVIFRYVLNRPVAWGEE
                     LASLLFAWTTFVGAFVGFRSRSHIAIDTLVIFLPRAVRDGLARLVDVAVLLLLGLFAW
                     QGLSLCLTTWSLEFPAMEISRGYLYLSLPVGACLMAVAVIEEWQERRVSHTRPSAEQG
                     TRS"
     gene            1842..>2083
                     /locus_tag="AC16G0_04130"
     CDS             1842..>2083
                     /locus_tag="AC16G0_04130"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR5098845.1"
                     /translation="MMVLVLIGVLLLLLALEFPVAIAMSGASVVYLLLRGDIPLVVVA
                     QRVTVGVDSFVLLAIPFFFLAGELMNQGGITKRLVDL"
BASE COUNT          375 a          635 c          695 g          378 t
ORIGIN      
        1 aggcgcgagg cgaggggctt gacgtggtgg gacccgtgcc gcccgacagc ttcttcccct
       61 tggccctgcg tggagacttc cggggcatgg tgttcatgta ccatgaccaa gccaacatcg
      121 ggcggaagat cctggggcgg gaccagcccg gcgtcagcct gttcctgggc atgccggtcc
      181 ccgtgggcac cgtgccccac ggcacggcct acgacatcgc ctggaagggg atcgccaagc
      241 acgccatgat cgtccgggcc ttcagcgtga cggcggccct ggcggcttcg aagtgaggga
      301 accggaagcg gacccaggtc cgcagacagg agggattgag atgcggcgcc atacaattct
      361 gtggatgatg atcgcgctgc tcttggtcgg gatcgccggt ccaggggcgg tgggtgcaca
      421 gcagccggtc ctgatcaagc tggggaacgt gcaggccacg ggggacatcg tgcaaaccgg
      481 cctgaagaag ttcgccgatc tggtgagcga gcggacgaag ggccgagttc aagttcagat
      541 cttccccgca tcgcagctcg gcacggaaca ggaactcttg gaaggggtgc agctcggcac
      601 ggtccacatg ttcgagggga gcaccggctc cgtggggcgg tttcttccgg acctggaggc
      661 cttcgccgca ccctatatct ggcgggacac ggaccacatg ctgaaggtgg tccgcgggcc
      721 catcggccag gaactggcgg acagactcgt gaaggccaag gggatgcggg tcctcgactt
      781 cggctggctc ttcggcaacc gctatctgac cacgaagtcg aagcccatcc acaagcccga
      841 ggacctcaag gggatgaaga tccgggtcca gccgacggcc atctatctgg agacgatcaa
      901 ggccatgggc gccaatccga cgacgatgga tttcaaggaa gtgtacctgg ggctgcagag
      961 cggcgtgatc gacggccagg agaacccgcc cttcgtcatt ttcaacaaca agctcttcga
     1021 ggtccagaag tacgtgatgc tcacggcgca catcacgcag aaccaggcga tcgtcatcaa
     1081 cgacaagttc taccagggcc tctccccgga tttccagaag atcctccagg acgcggccca
     1141 cgaggccgga aacttccaga acgagcttat cctgaagagc gagcaggagt acctggataa
     1201 gctcaaggaa aaggggatga tcatcgttca gccggacgtg aaggccttcc gcgaggcgac
     1261 caaggacgtc tggaagaagg tctcggagaa gtgggagccg ggcctgtacg agaagatcca
     1321 ggcggtgaag taacctggtg aggtaacctg aggatgaagc cgaccgtcgt ggaccgcctg
     1381 gtcaactggg gactggccgc ctgcctgggc acgatgaccg gggtggtctt cgtgagcgtc
     1441 atctttcgat acgtgctgaa caggcccgtc gcctgggggg aggagctggc ctcgctcctc
     1501 ttcgcctgga ccacctttgt gggggccttc gtcggcttcc gcagccgctc ccacatcgcc
     1561 attgataccc tggtcatctt cctcccacgg gcggtgcgag atgggctcgc ccgtctcgtg
     1621 gatgtggccg tgctgctcct gctcggcctg ttcgcctggc aaggcctaag cctctgcctc
     1681 acgacctgga gcctggagtt ccccgccatg gagatctccc ggggttacct ctatctctcg
     1741 ctgccggtgg gagcctgcct gatggcggtt gccgtcatcg aggagtggca ggagcgccgg
     1801 gtttcgcaca cgcgacccag cgcagagcag ggaacccgat catgatggtc ctcgtgctga
     1861 tcggcgtcct cctgctcctg ctggccctgg aattccccgt ggccatcgcc atgagcgggg
     1921 cctcggtggt gtacctcctg ctccggggag acatcccgct ggtcgtcgtg gcccagcggg
     1981 tgaccgtggg cgtcgactcc ttcgtccttc tggccatccc gttcttcttc ttggccggtg
     2041 agctgatgaa tcagggcggc atcaccaagc ggttggtgga cct
//
LOCUS       DBNGZC010000227         5081 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1878537, whole genome shotgun sequence.
ACCESSION   DBNGZC010000227 DBNGZC010000000
VERSION     DBNGZC010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1878537"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            382..1662
                     /locus_tag="AC16G0_04135"
     CDS             382..1662
                     /locus_tag="AC16G0_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098846.1"
                     /translation="MIRSSQRWVVASLIIGIAVLLAAGPAAAQVKIRFQTWHWGEKPV
                     FYALDEFKNEFNKNNPGIEVVADESRYGDKEAVYTAQSQAKAAADIAHFMHRAIPLFA
                     ERGYIMDLTPFIEREGGQKWLAQWDQTALEVCKYKGKIYCINDYVNPLALLYNTQHFK
                     EAGLDPSKPPTNPIELAEAAKKLTRSGRYGMGLIGSRQEGLFMRFNPFLWGAGAEYLT
                     PDSKRSALDTPEALEGFKAYVELFTKYKAVPPGVVEQGAQEVRTQVAHEKVSMEIGVI
                     QAPLIYKAINPNFKVDEVMAAAPIPSGKKRVSSVEYGLRVISAFTKHPEEAWKVYKSW
                     LSKEVQLRNFKIYGVMSARLDVRSSPEIANHKFAKVFAAQGQYGKIEPMIPEWPKIGD
                     AMITAVQEAFSGTKTPEQALKDAHTAANRALGVQ"
     gene            1874..3181
                     /locus_tag="AC16G0_04140"
     CDS             1874..3181
                     /locus_tag="AC16G0_04140"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098847.1"
                     /translation="MRMFRPSRLATAFSIAFTWAVILSAVPVAAQVKIRFQTWQWNQQ
                     PTVKALEEFQRAFNAANPGVQVVREDSKYGDKEAVFTAQSQAKAAADIAHFSIRPIRS
                     FADHGFLLDLTPFVEKEGGAKFLAQWDPVALEMCRYKGKLYCLPDNLDPVVLMYNIEH
                     FKQAGLDPDKPPTTWDQFVDYAKKLTRSTKGDGKIDRWGVGILGAREEGVFMRFDPWL
                     WGAGGDFLTSDGKRSALDTPEALAGFRYYVELLTKHKVVPPGVIEQGAQNVRTQLAHE
                     TVSMILNFSSEPYIVNVINPQLRALDVLRWAPLPVGKKKVTAAWFSLRVISANTKHPE
                     EAWKVYKAWYGRDQQVLNFKNDAFVSTRLDVRNAPEVKSHKFARFGLAQAPYVKWEPL
                     IPEWPKITDAMLTAIQEAFTGVKTPEQALKDAHLATNRALGAQ"
     gene            3336..4211
                     /locus_tag="AC16G0_04145"
     CDS             3336..4211
                     /locus_tag="AC16G0_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5098848.1"
                     /translation="MRSGRTWHDRQLGYLFVLPATLMLVAVLVFPAFITLKASLTPEG
                     GSGWHLTLGHYQKTFHDPVLLQTFKNTFAFVAWSMVGHFILGLGLALALNQSLPGQTF
                     FRLVALIPWTIPDVVAGIIFRWVLNPIHGALNPLIRMVIPSFPAEFQWLSSPTLAFPS
                     VIFANVWRGYPLVMVMLLAGLMAIPRELYEAAAVDGAGKLRRFFHITLPGLSTIILIA
                     LALDMVWEFRRFALVVTMTGGGPGTLTEVLSTLVYKTYFQFFTFEYASAMAIMMSAIL
                     FILSLPYIILVGRER"
     gene            4208..5062
                     /locus_tag="AC16G0_04150"
     CDS             4208..5062
                     /locus_tag="AC16G0_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5098849.1"
                     /translation="MKRSAASERQERRVDAVVSYGLLALSTTFALFPIVWMLSTSLKS
                     EAEALSLPIRWIPNQFTLQAYGEMWTLKPFARYFWNSMAVSGSTALISTIVGALAGYG
                     FSRFRFRGRTSLLAFFLATQMISGVLVIGPYFQLLAAVELYNTLTGLVIAYVTICLPF
                     AAWMSKGYFDSIPKELDEAGLVDGASRLQIFLRIICPIAVPGTVSTLLFAFLLAWQDL
                     LWALCLISIDEKRTVTLGVAFLVGEFVIKWPMLTAASLIGSLPTIILYLFLQRYYVEG
                     LARGAVKG"
BASE COUNT          922 a         1615 c         1573 g          971 t
ORIGIN      
        1 catcctggtg gatctgcagc gcaagggcat gcaggtcgtc atcccggatg cggagtcgtt
       61 ccgggtgaag gccaaaccag ccgtggaggt gctgttccag agggagtgga acgtcacgac
      121 gtggcaggaa gtgcttgcgc aataagagcg cgggctcggg tgggacaacc gaacgtgatt
      181 gacgagccag gggatgctct cccctggctc gttgcctgtc atgagcccgt ggatgtcttg
      241 ccgttggact tggtatgtcc tccatgccag cagcggaatt gccttgtcag gctcggcggg
      301 cttacggtag tgttcgcgtg gtgcgcaggc gcctcgcgta cgcgagctgc atttcttact
      361 cactttcaca ggaggtccgc catgatacgc agcagtcaaa ggtgggtggt ggcgtctctg
      421 atcatcggga tcgccgtcct cctcgccgcc ggcccggcgg cggcccaggt caagatccgg
      481 ttccagacct ggcactgggg cgagaagccc gtcttctatg ccctcgatga gttcaagaac
      541 gaattcaaca aaaataatcc agggatcgag gtcgtggcgg acgagagccg atatggcgac
      601 aaggaggcgg tctacaccgc ccagtcccag gccaaggccg cggccgacat cgcgcacttc
      661 atgcaccgcg ctatcccgct cttcgccgag cgcggctaca tcatggacct cacgccgttc
      721 atcgagcgag agggcgggca gaagtggctg gcccagtggg accagaccgc actcgaggtc
      781 tgcaagtaca aggggaagat ctactgcatc aatgactatg taaatccgct ggcgctgctc
      841 tacaacaccc agcacttcaa ggaggccggc ctcgatccga gcaagccgcc cacgaacccg
      901 atcgagctgg cggaggcggc caagaagctg acccggagcg gccgatacgg catggggctg
      961 attggatcgc gtcaggaagg cctgttcatg cgcttcaacc cattcctttg gggcgccggc
     1021 gccgagtacc tcaccccgga cagcaagcgc tcggccttgg acaccccgga ggcactcgag
     1081 ggcttcaagg cctacgtgga gctcttcacc aagtacaagg cggtcccgcc cggcgtggtc
     1141 gaacagggcg cgcaggaggt ccggacgcag gttgcgcacg agaaggtctc catggagatc
     1201 ggcgtcatcc aggccccgtt gatctataag gctatcaatc ccaacttcaa ggtcgatgag
     1261 gtgatggcgg ccgcgccgat tccgtccggc aagaagcgcg tcagctccgt cgagtacggc
     1321 ctgcgggtca tcagcgcttt caccaagcac cccgaggagg cgtggaaggt ttacaaatcc
     1381 tggctcagca aggaagtcca actccggaac ttcaagatct acggggtcat gtccgcacgc
     1441 ctggacgtca ggagctcgcc agagatcgcg aatcacaaat tcgccaaggt cttcgctgcg
     1501 cagggccagt acgggaagat cgagccgatg attcccgagt ggccgaagat cggcgacgcc
     1561 atgatcacgg cggtccagga ggcattctcg ggcacgaaga cacccgagca ggccctgaag
     1621 gacgcccaca ccgcggccaa ccgcgcgctc ggcgtccagt agggttcttt ggaagctttc
     1681 agccgtcagc gatcagctaa agaggggccg ggagcacatc ctggttcttc ctaaagctga
     1741 ccgctgacta ttgatggctt cccattgacc gcttcccatt gactccgccc gagccagggg
     1801 gaataatcca tccccctggc tcggtgtttt gcggtctggt ggctctgtgg caagcggcag
     1861 aaggaggggg aggatgagaa tgtttcgacc gtctcggctc gcgacggcat tcagtatcgc
     1921 gttcacctgg gccgtgattc tctctgccgt cccggtcgcg gcacaggtca agatccggtt
     1981 tcagacctgg cagtggaatc aacagcccac cgtgaaggcg ctggaggaat tccagcgggc
     2041 cttcaacgcc gccaatcccg gcgtccaggt cgtgcgtgag gacagcaagt acggagacaa
     2101 ggaagcggtc ttcaccgccc agtcccaggc caaggcggcg gccgacatcg cccacttcag
     2161 catccgcccg atccggagct ttgccgatca cggcttcctc ctggatttga cgcccttcgt
     2221 cgagaaggag ggtggggcca agttcctggc gcagtgggac cccgtcgcac tggagatgtg
     2281 ccggtacaag ggcaagctct actgcctgcc ggacaacctg gacccggtgg tcctgatgta
     2341 caacatcgag cacttcaagc aggccggcct ggatcccgac aagccgccca cgacctggga
     2401 ccagttcgtg gactacgcca agaagctgac ccgctccacc aagggcgacg gcaagatcga
     2461 tcggtggggc gtggggatcc tgggtgcacg cgaagagggc gtcttcatgc ggttcgaccc
     2521 gtggctctgg ggagccggtg gggacttcct cacttcggat ggaaagcgct ccgcgctgga
     2581 cacacccgag gcgctggcgg gcttcaggta ctacgtcgag ttgctcacga agcacaaggt
     2641 ggtgccgccg ggcgtgatcg agcagggcgc ccagaacgtg cggacgcagc tcgcccatga
     2701 gaccgtctcc atgatcctga acttctcttc tgagccgtat atcgtgaacg tcatcaatcc
     2761 acagctccgc gccctggatg tgctccggtg ggcgccgctt ccggtgggga agaagaaggt
     2821 gaccgccgcg tggttcagcc tccgtgtcat cagtgcaaac acgaagcatc ctgaggaagc
     2881 ctggaaggtc tacaaggcct ggtacggcag ggaccagcag gtcctgaatt tcaagaacga
     2941 tgccttcgtc tccacccgtc tggacgtgcg gaacgcgcca gaggtgaaga gtcataagtt
     3001 cgcgcgcttt ggcttggccc aggcgcccta cgtcaagtgg gagcccttga tccccgagtg
     3061 gcccaagatc accgatgcga tgctcaccgc catccaggag gcctttaccg gcgtgaaaac
     3121 gcctgagcag gccctgaagg atgcgcatct cgcgacgaat cgggcgctcg gcgctcaata
     3181 ggtcgtcgag cagtcagcgg tcagccgtct gcgatcagcc aaagacggga gcacgcagac
     3241 gtccaggatt cattcgttct tgctgaaagc tgaccgccgc tgactgctga ccccttcggt
     3301 ggcccaggcc cgctatctac gaacgggagt tcggcatgcg ctcaggccgc acctggcacg
     3361 accgccagct cgggtatctg ttcgtcttgc ccgccacgct gatgctggtg gcggtgctgg
     3421 tcttccccgc cttcatcacg ctgaaggcga gcctcacgcc cgagggcggc agcggctggc
     3481 acctcacgct tggccactat cagaagactt tccacgatcc agtcctgctc cagacgttca
     3541 agaacacctt cgccttcgtg gcctggagca tggtcggcca ctttattctg ggattgggcc
     3601 tggcgctggc gctgaaccag tccctcccag gccagacctt cttccggctg gttgctctta
     3661 tcccctggac cattccggac gtcgtggccg gcatcatctt ccgctgggtg ctgaacccga
     3721 tccacggcgc cctgaacccg ctgatccgga tggtcattcc aagtttcccg gccgagttcc
     3781 agtggctctc cagcccgacg ctagccttcc cgtccgtgat cttcgccaat gtctggcgcg
     3841 gctatccact cgtgatggtc atgctcctcg ccgggctcat ggcgattccg cgcgaattgt
     3901 atgaggcggc cgcagtggac ggggccggca agctccggcg ctttttccac atcacgctcc
     3961 cgggtctcag caccatcatc ctgatcgcgc tggcgctcga catggtgtgg gagttccgcc
     4021 ggttcgcgct ggtagtgaca atgacgggtg gcggcccggg gacactgacg gaggtgctgt
     4081 cgacactcgt ctataagacg tacttccagt tcttcacctt cgagtacgcc tcggccatgg
     4141 ccatcatgat gagcgccatc ctcttcatcc tgagcctccc ctacatcatc ctggtgggga
     4201 gggagcgatg aagcgatcag cggcctcgga gcggcaggaa cggcgggtcg atgcggtcgt
     4261 ctcgtacggc ctgctggccc tgtccacgac cttcgccctc ttccccatcg tctggatgct
     4321 ctcgacatcc ctcaagagtg aagcggaggc gttgagcctc ccgatacgct ggatccccaa
     4381 ccaattcacg ctgcaggcat acggcgagat gtggacgctg aagccctttg cgcgctactt
     4441 ctggaacagc atggcggtct ccggctcgac cgcactgatc tcgaccattg tgggtgccct
     4501 ggcgggctat ggcttctcgc gttttcgctt ccgcggccgg accagcctgc tggccttctt
     4561 cctcgcgacc cagatgatct ccggcgtcct ggtgatcggc ccatacttcc agttgctggc
     4621 ggcagtcgag ctgtacaata cgctcaccgg gctggtcatc gcctacgtga ccatctgttt
     4681 acccttcgcg gcgtggatgt ccaagggcta cttcgactcc atcccgaagg agctggacga
     4741 ggccggcctg gtggatggcg cctcgcggct ccagatcttc ctgcggatca tctgcccgat
     4801 cgcggtgccg ggcaccgtct cgacgctgct cttcgccttc ctgctggcct ggcaggacct
     4861 gctctgggcg ctctgcctca tctccatcga cgagaagcga accgtgacgc tgggcgtcgc
     4921 ctttctcgtc ggcgagttcg tgatcaagtg gcccatgctc accgccgcct ccctcatcgg
     4981 aagcctcccg accatcatcc tctacctttt cctgcagcgg tattatgtgg aaggcctcgc
     5041 gaggggcgcg gtgaagggat aggccaaaca gatacgcgct c
//
LOCUS       DBNGZC010000228         2965 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_214712, whole genome shotgun sequence.
ACCESSION   DBNGZC010000228 DBNGZC010000000
VERSION     DBNGZC010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_214712"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..785
                     /locus_tag="AC16G0_04155"
     CDS             144..785
                     /locus_tag="AC16G0_04155"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR5098850.1"
                     /translation="MAPGLPAEPPIVPPGSDAGGSPPPQSPREEPVVSNAVLGMLMFL
                     AFETMFFAGLLGAFLVFRLESTSWPPPGEPYLPIGVTWVNTGILLASACTMRWAHRAI
                     RVGNRAGLVQGLGLTALLGTTFLSVQGSEWIRLVQYGLTLQSGTYGATFYTLIGCHGI
                     HVLAAVLWLAVVLVLAVRGRFSPERHVAVQLCKMYWMFVVGLWLVLFPSVYLM"
     gene            847..1134
                     /locus_tag="AC16G0_04160"
     CDS             847..1134
                     /locus_tag="AC16G0_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098851.1"
                     /translation="MKRLAQVVTFGLIGPALLFPAIAEACAVCGTGLGVAGDPTARGF
                     YWGILFLMAMPFAVAGSIGAWLVYRYWCASAGGWQKALGAASARTHKETGR"
     gene            1131..1748
                     /locus_tag="AC16G0_04165"
     CDS             1131..1748
                     /locus_tag="AC16G0_04165"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR5098852.1"
                     /translation="MSEAAVLHPEIEPAESPLTPESWGKLGMWIFLAGDAMSFGALLS
                     GYGALRYGSADWPHPSSVLGVGLTAFMTFLLICSSVTMVLALAGIQRGDMVALKKFLA
                     LTILGGVSFLGMQAFEWTHLIREGLGLTGNPWGASLFGTTFFIITGFHGAHVTGGVVY
                     LSCYLIRSLLGGLKQDHANQIEIAALYWHFVDLVWILVFTFVYLL"
     gene            1959..2285
                     /locus_tag="AC16G0_04170"
     CDS             1959..2285
                     /locus_tag="AC16G0_04170"
                     /inference="COORDINATES: protein motif:HMM:NF015582.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HIR5098853.1"
                     /translation="MAQAHKQPNYIAIFWWLLALTILEVAVIFTPLAWVLIAILLVGL
                     AISKASLVAMYFMHLKFERTTLGLIAMTPLLLCVLLVFALLPDLSGAIHRAVGTPAPP
                     AAPASQ"
     gene            2463..>2965
                     /locus_tag="AC16G0_04175"
     CDS             2463..>2965
                     /locus_tag="AC16G0_04175"
                     /inference="COORDINATES: protein motif:HMM:NF025615.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HIR5098854.1"
                     /translation="MGKPIIPKEHGAWAVLYGSYLVGLGVAARVNLPVVLLLIGVTAA
                     AFADGPLAILLRSASGPAHADRRRRALVWFLVYAGLAAMAIAPLLTVFRMTFLLPFGV
                     ATVVFLLVRGLLVRGRDDRSLVGELMGTAGLTMIGPIAHAVASGEVKFVGLVLWLPLF
                     LFFASGI"
BASE COUNT          451 a          895 c          976 g          643 t
ORIGIN      
        1 ttcgcatcgc tcgtgtacct cccggccctg ctggtcctga tggctctcac caagttgccg
       61 tcctgatcgg cgggggttgg agacgggcgg ctggaaagaa tatagtggca cagggaaccc
      121 tgacggcccc ggcgttggag gagatggcac ccggcctgcc ggcggagccg ccgatcgttc
      181 cccctggatc ggacgctgga ggctctccac ccccgcagtc accccgcgaa gagccggtcg
      241 tcagcaatgc cgtcctgggc atgctgatgt ttctggcttt cgagacgatg ttcttcgccg
      301 ggctgctggg ggcctttctg gtcttccggc tggagagcac gagctggccg ccccccgggg
      361 agccgtacct gccgatcggg gtgacgtggg tcaacaccgg gattctgctg gccagcgcct
      421 gcaccatgcg ctgggcccac cgggcgatcc gggtcgggaa ccgagcgggc ctcgttcagg
      481 gcctcggact cacggcgctc ctcgggacca cctttctgtc agtgcaggga agcgaatgga
      541 tccgcctggt gcagtacggt ctgaccctcc agagcgggac ctacggcgcg accttttaca
      601 cgctgatcgg gtgccatggc atccacgtcc tcgcggcggt tctatggttg gcagtagtcc
      661 tggtcctggc cgtgcgaggc cggttttcgc cggagcggca cgtcgccgtc cagctctgca
      721 agatgtactg gatgttcgtg gtgggcctgt ggcttgtcct gttcccgtcc gtctacttga
      781 tgtgaggtgg gcggacaaac gacgcctggt gtcgagtggt ttcggcccca gcacctgcga
      841 gcgagcatga aacgcctagc ccaggtggtc acgttcggtc tcatcggtcc ggcgctcctg
      901 ttccctgcga tcgcggaagc gtgcgccgtc tgcgggacgg gtctcggcgt cgcgggcgac
      961 ccgaccgctc gaggatttta ctgggggata ctctttctca tggcaatgcc ctttgccgta
     1021 gcaggatcca ttggcgcttg gttggtgtac cggtactggt gtgcaagtgc gggtggctgg
     1081 cagaaggcgc tcggcgcggc gtccgccagg acacacaagg agactggacg gtgagcgagg
     1141 cggctgtgct tcacccggaa atcgagccgg cggaatcccc ccttacccca gaaagctggg
     1201 ggaagctcgg gatgtggatc ttcctggcgg gagacgccat gtccttcggg gccctgctct
     1261 ccggatatgg ggcgctgcgc tacgggagtg ccgattggcc gcatccgtcc tcggtactgg
     1321 gggtggggct cacggccttc atgaccttcc ttttgatctg cagcagcgtg accatggtgc
     1381 tggccctggc cgggatccag cgtggcgaca tggtggccct caagaagttc ctggccctca
     1441 caatcctggg gggggtgagc tttctaggga tgcaggcctt tgagtggacg cacctgatcc
     1501 gcgagggtct ggggttgacc ggaaacccgt ggggggcatc cctgttcggc acgaccttct
     1561 tcatcatcac cggatttcat ggggcgcacg tcacgggcgg cgtggtctat ctctcgtgct
     1621 atctgatccg gagccttctg ggcggtctga agcaggacca cgccaaccag atcgagatcg
     1681 ccgccctgta ctggcacttc gtggacctgg tctggatcct ggtcttcacc ttcgtgtacc
     1741 tgctgtagcc cgcatctttc gcgatgaggc ttcgcgaatg atgcgggaat gatcaaatcc
     1801 caatggcaaa tgaccaaaga ggaggggaca gctgatactt ggatcggaag ggcgcgtcgc
     1861 gcgcgccgac caccatttgt cattggaaaa tggtcattga gcccggatga gtcactggta
     1921 ctttcgtgaa tcatccggac tagggagtgg gaagagctat ggcgcaggcg cacaaacagc
     1981 caaactacat cgccatcttt tggtggctcc tggccttgac gatcctcgag gtcgctgtga
     2041 tcttcacgcc cctggcgtgg gtgctgatcg cgatcttgtt ggtgggtctg gccatatcca
     2101 aggcctccct ggtcgccatg tacttcatgc acctgaagtt cgagcgaacg accctggggt
     2161 tgatcgcaat gaccccgctg cttctatgtg tgctgctggt gttcgcgctc cttcccgatc
     2221 tctccggagc catccaccgg gctgtcggaa cgcctgcacc accagcggcg cccgcctccc
     2281 agtgaccccg gattgccggc tcgcgctccc attatgatct agatcatagc gggaccctgg
     2341 acgggaccgt accatgtcga tcgagcggag gactcccgct gagacggaag tcccctgcga
     2401 cgacgggaga ctgggctggc gatttcgccg ttatagaggt atgcgagttg gatcttcaag
     2461 gcatgggcaa accgattatc ccgaaggagc acggcgcctg ggcggtgctg tacggatcgt
     2521 acctcgtagg tctgggagta gcggcacggg tgaaccttcc agtagtgctc ctcctcatag
     2581 gtgtcaccgc cgccgccttt gctgacggcc cccttgcgat cctgttgcga tcagcttctg
     2641 gtccagctca cgcggaccga aggcgccggg ccctcgtctg gttcctggtc tatgccggct
     2701 tggccgcgat ggctatcgca ccgctcctga cggtatttcg aatgaccttt ctcctcccct
     2761 tcggcgtggc gacggtagtc ttcctgctgg tacgcgggct tctggtccgc ggccgggacg
     2821 accgatccct cgtgggcgag ctgatgggga cggccgggct gactatgata ggtccgattg
     2881 cgcatgctgt ggcttcgggg gaagttaaat tcgtcgggct tgtgctctgg ctgccactgt
     2941 ttctcttctt tgccagtggg atctt
//
LOCUS       DBNGZC010000229         2089 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_605902, whole genome shotgun sequence.
ACCESSION   DBNGZC010000229 DBNGZC010000000
VERSION     DBNGZC010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_605902"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..>2089
                     /locus_tag="AC16G0_04180"
     CDS             178..>2089
                     /locus_tag="AC16G0_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HIR5098855.1"
                     /translation="MKSDVSEEELTKLLAKVDKESTFRKLIGFDYRLVYWIAVAFSSF
                     QLYTAMFGAYPAQIQRSVHLSFAFTLVFLLYPFRGKTAVNKLRWQDYLFAFFAAFVGL
                     YLTINYTRLMETAGDYTRIDMLVGICGTLLTLEAARRVVGLPMVCIASSFLAYAYLGA
                     YFPGFLSHRGYSIERIVSHMYFTTEGILGIPLGVSSTFIFLFVLFGAFLEKTGIGKLF
                     IDIADSIAGWASGGPAKVAVITSALEGTVSGSSVANTVGSGSFTIPMMKKLGYRPEFA
                     GAVEASASTGGQIMPPVMGAAAFLMSEFIGVPYIEIAKAAAIPACLYFFGIFIEVHFE
                     AKRTGLRGMPWDQIPRIWTVLKERGHLFVPLIVIIYVLTEGYTPSYAALVGLALSVVA
                     GMLKKATRMSIPEIFNALEAGARSALGVAIACATAGIIVGVVTLTGLGLKMANGLIDL
                     AGGNLLLTLFFTMITSLILGMGVPTTANYVITATMAAPALVMLAVPKLAAHLFVFYFG
                     IIADITPPVALAAYAGAGIARANPFWTGVTASKLAVGAFIVPYIFVYNPAMVLIGTTP
                     YLLAHNLITACLGMFGVGTAMIGFCLARMNWFERVWFFVGGLMLIEPGLLTDAIGVLM
                     LAIGLAIQWRKSK"
BASE COUNT          336 a          686 c          632 g          435 t
ORIGIN      
        1 tcacatcctg accctcaagg ggagagccat ccccctggcg gaccttgccg agccggggac
       61 ccccctggcg tttcgcgtgg tttcgcgaac gatcccctca gcctcgaaag gcaggtgcct
      121 gcggtgagcg acaaggagtc gtccaagggg cagcccgaga tcctccgcac ctccgacatg
      181 aagtcggacg tctccgaaga agaactgacg aaacttctgg ccaaggtcga caaggagtcg
      241 actttccgca agctcatcgg cttcgactat cggctggtct actggattgc cgtcgccttc
      301 tccagcttcc agctgtacac ggccatgttc ggcgcctatc ccgcgcagat ccagcggtcc
      361 gtgcacctct ccttcgcctt caccctggta ttcctgctct accccttccg gggcaagacc
      421 gccgtcaaca agctgcgctg gcaggattac ctgttcgcct ttttcgccgc cttcgtcggg
      481 ttgtatctga cgatcaacta cacccgcctg atggagacgg cgggcgacta caccaggatc
      541 gacatgctgg tcgggatctg cgggacactg ctcacgctcg aggcagcgcg ccgggtggtc
      601 ggactgccca tggtctgcat cgccagcagt ttcctcgcct atgcgtacct tggtgcctac
      661 ttccccggct tcctgtccca ccggggctat tcgatcgagc ggatcgtctc gcacatgtac
      721 ttcaccacgg aagggattct ggggattccc ctgggggtgt cctcgacctt catcttcctc
      781 ttcgtcctct tcggcgcatt tctggagaag acgggcatcg ggaagctgtt catcgacatc
      841 gctgattcca tcgccggctg ggcctcgggc gggcccgcca aggtggcggt gatcacgagc
      901 gcgctggaag ggaccgtctc gggcagttcg gtggccaaca cggtgggctc cggcagcttc
      961 acgattccca tgatgaagaa gctcgggtat cgccccgagt tcgccggcgc ggtggaagcc
     1021 tcggcctcca ccggtggcca gatcatgccc ccggtcatgg gcgccgccgc gttcctcatg
     1081 tccgagttca tcggcgtgcc ctacatcgag atcgccaagg cggcggccat ccctgcctgc
     1141 ctctacttct tcggcatctt catcgaggtg catttcgagg ccaagaggac cgggcttcgc
     1201 ggcatgccct gggatcagat tcccaggatc tggaccgtac tgaaggagcg ggggcacctc
     1261 ttcgtgccgc tgatcgtcat catctacgtg ctgaccgagg gctacacgcc gagctatgcg
     1321 gctctggtgg gccttgcctt atcggttgtg gccggcatgc tgaagaaggc cacccggatg
     1381 agcatcccgg aaatattcaa cgccttggag gcgggggccc ggagcgccct gggggtggca
     1441 atcgcctgcg ccactgccgg gatcattgtc ggcgtcgtta cgctcaccgg gcttggtctc
     1501 aagatggcga acggcctgat cgacctggcc ggagggaacc tcctcctcac gctattcttc
     1561 acgatgatca cgtccctcat cctggggatg ggggtgccga ccacggccaa ctacgtgatc
     1621 accgcgacta tggccgctcc agccctcgtc atgcttgcag tcccgaagct cgccgcacac
     1681 ctcttcgtct tctactttgg catcatcgcc gacatcacgc cgccggtagc cctggcggcc
     1741 tatgcagggg cggggatcgc cagggccaat ccattctgga cgggtgtcac ggcctcaaaa
     1801 ctggccgtcg gggcgttcat cgtcccctac attttcgtgt acaacccggc catggtgctg
     1861 atcgggacaa ccccatacct gctcgcgcac aatttgatca ccgcctgcct ggggatgttt
     1921 ggcgtcggga cggcgatgat cgggttctgc ctggcccgca tgaactggtt cgagcgggtg
     1981 tggttcttcg tgggaggcct gatgctgatc gagccaggcc tgctcacgga tgccatcggc
     2041 gtgctgatgc tggccatcgg cctcgcgatc cagtggcgga agtccaaga
//
LOCUS       DBNGZC010000230         2650 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_410393, whole genome shotgun sequence.
ACCESSION   DBNGZC010000230 DBNGZC010000000
VERSION     DBNGZC010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_410393"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="AC16G0_04185"
     CDS             complement(<1..266)
                     /locus_tag="AC16G0_04185"
                     /inference="COORDINATES: protein motif:HMM:NF014750.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5098856.1"
                     /translation="MQGAFNGKFLRVDLTKGTTAVEEVPALIYRMYLGGNTMAAYFLA
                     KELKPGVDPLGPDNVMVFATGPCNGSPISGTNRFACATKSPLTGG"
     gene            complement(452..742)
                     /locus_tag="AC16G0_04190"
     CDS             complement(452..742)
                     /locus_tag="AC16G0_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018055042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR5098857.1"
                     /translation="MSKHPAVYILASRRNGTLYIGVTSDLVKRIWEHRNNMVEGFTKR
                     YGVHQLVWYELHDYMEAAIQREKRLKDWKRAWKLILIETTNPGWRDLYQTLG"
     gene            complement(837..1781)
                     /locus_tag="AC16G0_04195"
     CDS             complement(837..1781)
                     /locus_tag="AC16G0_04195"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR5098858.1"
                     /translation="MPEQIAMREAFGQTLVALGKRVPEILVLDADVSPSTRAVLFAQA
                     FPERFIQVGIAEQNMVGMAAGLSTLGFIPFTSTFACFAAKRNLDQIRIVVAQPKTNVK
                     ITGHYAGLIAGKTGKTHLSIQDMAIMRSMPNMTVIAPADGVEVAKAMEVMVAVHGPMY
                     LRLARDPSPVIFDQSYRFELGKAVRLRDGADVTLITTGTQTPRTLEAADMLKAEGIAA
                     LVLHVPTLKPLDVDAIVAAADRTGLVVTAEEHSILGGLGGAVAEVLSEHRPTPVLRVG
                     VKDVNAESAPNDDLLKRYGLTARHVADAARAHLAKWRR"
     gene            complement(1787..2644)
                     /locus_tag="AC16G0_04200"
     CDS             complement(1787..2644)
                     /locus_tag="AC16G0_04200"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015282867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR5098859.1"
                     /translation="MPVKGKGLIIMTQKDSATELQAIANRLRLQTLEMIWHKQTGHPG
                     GSFSLAEILACLFFRVLRIDPKRPDWPERDRFILSKGHAAPIYYATLAERGFFPKETL
                     ETYDELDSILQGHPDMHTPGVDISSGSLGQGLSPGVGLAWGARFKGMDFRTYVVLGDG
                     EIQEGQVWEAAMAASCYKLDNLTAIVDCNRIQLSDFIQKAMLVDPLADKWRAFGWQVV
                     ECDGHNVQAILNALEELQQVKGRPGVLLAHTVKGQGVSFMENKPAWHAKAPNEQEYQQ
                     AKAELTGRR"
BASE COUNT          454 a          869 c          803 g          524 t
ORIGIN      
        1 ccgcccgtga ggggagactt ggtcgcgcag gcgaagcggt tggtgccgga gatgggactc
       61 ccgttgcacg gaccggtggc gaagaccatg acgttatccg ggcccagggg gtctaccccc
      121 ggcttcagct cctttgcgag gaagtaagcc gccatggtgt tcccgcccag gtacatccgg
      181 tagatcagcg cggggacttc ctccaccgcg gtcgttccct tcgtcaggtc gacccgcagg
      241 aactttccgt tgaatgcgcc ttgcatgctc agctcctcct atgttgtgat ggtagatgcc
      301 ggacgcccgg ccggccgctt tcatgaaatc ggattgaacc cccttcgcga ccctcgccct
      361 ctgcgttcct ctctccccca gggcataagg actactagca caaaccggcg tctccttctt
      421 gctcgtcatt ccggcgaaag ccggaatcca gtcaaccaag tgtctgatac aagtcccgcc
      481 aaccagggtt ggtcgtttcg atcaatatca gtttccaggc acgtttccag tccttcaacc
      541 tcttctccct ttgaatcgca gcctccatgt agtcgtgcag ttcataccaa acgagttgat
      601 ggacgccata gcgtttggta aacccctcaa ccatattgtt cctgtgctcc cagatcctct
      661 tcacgagatc cgaagtcaca ccgatataga gcgtaccatt cctcctgctc gcaagaatgt
      721 acacggcggg gtgcttactc actccctcca actctgcctc ctggattccg gctttcgccg
      781 gaatgacgaa acagggagcg aggccctctt cggatggtgt caagggacga cctggcctac
      841 ctccgccatt tggccaggtg tgcccgcgcg gcgtcagcca cgtgacgggc cgtgagcccg
      901 tacctcttga gcaggtcgtc gttgggtgcc gactcggcgt tcacgtcctt cacgccgacc
      961 cgcagcacgg gggttggccg atgctccgac aggacctcgg ccacggcccc gcccaggccg
     1021 cccaggatgc tgtgctcctc cgcggtgacc accaggcccg tgcggtcggc cgcggccacg
     1081 atggcatcca cgtccagagg cttcagggta ggcacgtgca gcaccaaggc ggcgatcccc
     1141 tcggctttca gcatgtcagc cgcctccagc gtgcgaggcg tctgcgtgcc ggtggtgatg
     1201 agggtcacgt cggccccatc gcgcagacgg accgccttgc ccagctcgaa ccggtaggac
     1261 tggtcgaaga tcaccgggct ggggtcccgc gccaggcgca ggtacatggg tccgtggacc
     1321 gcaaccatga cctccatggc cttggccacc tcgacgccgt ccgcgggggc gatgaccgtc
     1381 atgttgggca tgctccgcat gatggccatg tcctggatgg acagatgggt tttcccggtc
     1441 ttgcctgcga tgagtccggc atagtggccg gtgatcttga cgttggtctt gggctgggcc
     1501 accacgatgc ggatctggtc caggttccgc ttggcggcga agcaggcgaa ggtagaggta
     1561 aacgggatga atcccagcgt ggagaggccg gcggccatgc cgaccatgtt ctgctccgcg
     1621 atacccacct ggatgaatcg ctcgggaaac gcctgagcga agaggaccgc ccgggtggag
     1681 ggggacacgt cggcatccag caccaggatc tccggcacac gcttgcccag cgccaccagg
     1741 gtctgcccga aggcctcacg cattgcgatc tgttccggca tggcgctcac ctcctccccg
     1801 taagttcggc cttggcctgc tggtactctt gctcgttggg agccttggca tgccaggcgg
     1861 gcttgttctc catgaaggag acgccctgac ctttcaccgt gtgggccagc aggacgcccg
     1921 gccgaccctt cacctgctgc agctcctcca gagcgttcag gatcgcctgg acgttgtggc
     1981 cgtcgcactc gacgacctgc cagccgaagg cgcgccactt gtcggccagc gggtccacga
     2041 gcatggcctt ctggatgaag tcgcttaact ggatgcggtt gcagtccacg atggcggtga
     2101 ggttgtccag cttgtagcaa ctggccgcca tggccgcctc ccagacctgc ccctcctgga
     2161 tctccccatc tcccaagacg acgtacgttc ggaaatccat tcccttgaag cgcgcgcccc
     2221 aggcgaggcc cacgccgggc gacaacccct gccccaggga gccggaggat atgtccaccc
     2281 cgggtgtgtg catgtcagga tgcccttgca ggatggaatc cagctcgtca tacgtctcca
     2341 gtgtttcctt gggaaagaag ccgcgctcgg ccagcgtggc atagtagatg ggggctgcat
     2401 ggcccttgct gaggatgaac cggtcgcgct ccggccagtc cggccgcttc ggatcgatcc
     2461 ggaggaccct gaagaacaag cacgccaata tttccgccag ggagaacgac ccgccgggat
     2521 gaccggtctg cttgtgccag atcatctcca gggtctgaag gcggaggcga ttggcaatcg
     2581 cctggagctc agtagcagag tccttctgcg tcatgatgat gagtcctttc cctttgacgg
     2641 gcattccacc
//
LOCUS       DBNGZC010000231         3161 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_19938, whole genome shotgun sequence.
ACCESSION   DBNGZC010000231 DBNGZC010000000
VERSION     DBNGZC010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_19938"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..656)
                     /locus_tag="AC16G0_04205"
     CDS             complement(165..656)
                     /locus_tag="AC16G0_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HIR5098860.1"
                     /translation="MPTPTMIAVIGENDPPPELVALAEAVGAEIAAAGAVLVCGGLGG
                     VMEAACRGAKGRGGTSIGILPGTRREDANRYVTYPVPTGLGHIRNILVVRTAQAVIAI
                     GGKYGTLSEIAFAKIDGTPVIGLQTWELKRHGLPDGPIQQAADAKQAVAMALEAARHR
                     AGQ"
     gene            complement(779..1327)
                     /locus_tag="AC16G0_04210"
     CDS             complement(779..1327)
                     /locus_tag="AC16G0_04210"
                     /inference="COORDINATES: protein motif:HMM:NF025819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008235 - metalloexopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HIR5098861.1"
                     /translation="MEIYLSENAFVGLLVSSIEVYRRECFGILLGHRESDRIMVEFAV
                     PYQSAKRKFQEVHMDWYRSQRVQEAVQTTSRWDLVGDYHSHPMYGDKKATTKLSGVDQ
                     KDFRDDGISIIVAINDGHRQQRWGYVKGGLISGSINGYALRIAAYHKEGEVIARVPLV
                     CPYAVGFMAKTKGPTPVEVRPI"
     gene            complement(1420..2220)
                     /locus_tag="AC16G0_04215"
     CDS             complement(1420..2220)
                     /locus_tag="AC16G0_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098862.1"
                     /translation="MDGAGRLLLLQILQARQQACRCLRTWRETDRLLAVLSGIIEPEL
                     RLHLLGEYLHERSPEEAAWTISRLWDRMTGGDRTVQGICLGLLDLNRLARVVPSGYLN
                     AVRDALERQGETSAGLLGGATKRRHAAETEIAPPPKEPVGYRISLARRPVPRLIERLL
                     FDPEPRVIQTVLANPRLSETEVVKLAASRRAPPEALEAIAADDRWISRYPVKVALANN
                     PAASSRLVLRLLPYLMRQDLRDLAAGAFNATVRQQASALLTRRQGSEG"
     gene            complement(2364..2439)
                     /locus_tag="AC16G0_04220"
     tRNA            complement(2364..2439)
                     /locus_tag="AC16G0_04220"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2404..2406),aa:Ala,seq:cgc)
     gene            complement(2824..>3161)
                     /locus_tag="AC16G0_04225"
     CDS             complement(2824..>3161)
                     /locus_tag="AC16G0_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012383.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HIR5098863.1"
                     /translation="ANGGTEVTLQSEYRAFFGEGGRVLIAEDILTTGETVKQLVRLVE
                     AMGGRVVGIGAIWRRSTRVKFKYPLFTVVTRDFPTYAPGECPMCKQGIPISISQSAGD
                     GGPDDEAED"
BASE COUNT          592 a         1018 c          955 g          596 t
ORIGIN      
        1 agcgcgcatc ccagaacgca gccgcattct caggctcgac cactcgcttc atgagagcct
       61 cacggccaga accgatttat tgcagattac gcagatatgc agaatgacat gaatacgaaa
      121 gaatctgcgt gaatctgcga aatctgcgga tcaaggaagt tctttcattg tccggcgcgg
      181 tggcgggcgg cctccagggc catggccacg gcctgcttgg cgtccgccgc ctgctgaatg
      241 gggccgtccg gcaacccatg gcgcttcagt tcccatgtct gcaagccgat gacgggggtg
      301 ccgtcgatct tggcgaaggc gatttcggaa agcgtcccgt acttgccgcc gatggcgatg
      361 accgcctggg cggtccggac cacgagaata ttgcggatgt gacccaagcc ggtgggcacc
      421 ggataggtta catatcggtt tgcatcttcc cgcctggttc ccggcagaat cccgatgctc
      481 gttccgccgc ggcccttggc gccgcgacag gcagcttcca tcacgccgcc gaggcccccg
      541 cagaccagga cagccccggc ggccgcgatc tccgccccaa cggcttctgc cagggcgacc
      601 aactcggggg gtgggtcgtt ctcgccaatg acggcgatca tcgtgggggt gggcatgaag
      661 cgctccgacc atgcgccaat tgaaaaacga caacggacaa ctgacacttg aaaacgcaga
      721 tcgtcgttgt cagtttttca ttgtcggttt ggaatcttgc attgctgaag ccgcagcctc
      781 agatgggccg cacctccacc ggtgtgggcc ctttggtctt ggccatgaaa cctacggcgt
      841 agggacacac caaggggacc cgcgcgatga cctccccctc cttgtggtag gcagcgatgc
      901 gaagggcata gccgttgatg cttccggaaa tcaggccgcc cttcacgtat ccccagcgct
      961 gctgccggtg gccgtcgttg atggcgacga tgatggagat tccgtcgtcc cggaaatcct
     1021 tctgatccac gcccgagagc ttggtggtgg ccttcttgtc tccatacatg ggatgcgaat
     1081 ggtagtcgcc caccaggtcc caccgcgagg tcgtctggac cgcctcctgc acccgctggc
     1141 tgcggtacca gtccatgtgc acttcctgga acttccgctt ggcggattga tagggaacgg
     1201 cgaactccac catgattcga tcggattcac ggtgccccag gaggataccg aagcactcac
     1261 gccggtacac ctcgatgctg gacaccagga gacctacgaa cgcattctca ctgagataga
     1321 tttccatcag gccttgctcc aagcaaaacg acgactgtac aaatgcggac gttcctacgt
     1381 tgcatgacgc cgctggtgct gcggctcttt ccggtggtcc tacccttccg acccctggcg
     1441 ccgggtaagt agggcgctgg cctgctgccg tacggtggcg ttgaatgcgc cggcggccag
     1501 gtctcgcaga tcctgccgca tcagatacgg taagagtctc agaaccagcc ggctcgacgc
     1561 ggcagggttg ttcgccaaag ccaccttgac tgggtagcgg gaaatccacc gatcgtccgc
     1621 tgcgatcgcc tcgagtgcct ccggcggtgc gcgccgggag gccgccaact tgaccacctc
     1681 cgtctcgctg aggcggggat tggccagtac cgtctgaatc acccgcggtt cggggtcgaa
     1741 cagcagccgc tcgatcaggc gcgggaccgg gcgcctcgcc aatgagatac ggtatcctac
     1801 cggctccttg gggggaggag cgatctcagt ctccgcggca tgtcgccgct tcgtcgcccc
     1861 gcccagcagg cctgcgctgg tctcaccctg tctttccagg gcgtcccgga cggcgttcag
     1921 atagccactc ggcaccacgc gcgccaggcg gttcaggtcg agcagcccga gacagatccc
     1981 ttgcaccgtg cggtctcccc cggtcatccg atcccacagt cgggagatcg tccaggccgc
     2041 ctcctcgggg gaacgctcat gcagatactc tccaagaaga tgcaggcgca gctccggctc
     2101 gatgatcccg gagagaaccg cgagcagtcg atccgtttcg cgccacgtcc ggaggcaccg
     2161 gcaagcctgc tggcgagcct gcagaatctg caagaggagg agcctaccgg cgccgtccat
     2221 gggcgaggat cacgagggtt attggaacct acgatcagaa gggcgtttgg ccgggtgacg
     2281 ttccgtcagg atccttccca cgactggaag atcagggctg tcctgcgctt cacaccgggc
     2341 ggcggagacg agctacccag aactggtgga gggtaaggga ttcgaaccct cgacctccgc
     2401 gttgcgaacg cggcgctctc ccagctgagc taaccccccg cccgaccacg aacccaaccg
     2461 atcgtggcgc tgcttctccg gcaggcttgc ttagattccg tccagtcgag agcgattcgg
     2521 ggatggccac ccctgggtga cccggaaggc cacgatctgg ccgaccgcca tcgggccctg
     2581 aatctgaccg caggctctag ggccgcgggg ccgcgggcgc gaccctggtc accactatac
     2641 tcaactccac ccagcactgt ccaggggatt ctggaggggg gatcggaggc catacctact
     2701 aaagtctgac attctgtctg gctggattga aaagctactc gatgccggaa tgaaggtcag
     2761 acaacgtcag caagatattg gttctattgg ttatgcagag gctttctcga tggtcaaact
     2821 gacttagtcc tcagcttcat cgtcaggtcc tccgtcccca gcactctgac tgatactgat
     2881 ggggatgccc tgcttgcaca tggggcactc ccctggcgca taggtgggaa agtcacgagt
     2941 cacgaccgta aagagtgggt acttgaactt gacccgggtg cttcggcgcc aaatggcccc
     3001 gataccgacg actcgacccc ccatggcctc caccagcctg acgagttgct tgaccgtctc
     3061 gccggtggtc aggatatcct cggcgatcag cacgcggccg ccctcaccaa agaaggcccg
     3121 gtactccgac tggagcgtaa cttccgttcc cccgttagcc g
//
LOCUS       DBNGZC010000232         4172 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1975349, whole genome shotgun sequence.
ACCESSION   DBNGZC010000232 DBNGZC010000000
VERSION     DBNGZC010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1975349"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="AC16G0_04230"
     CDS             complement(<1..190)
                     /locus_tag="AC16G0_04230"
                     /inference="COORDINATES: protein motif:HMM:NF025887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR5098864.1"
                     /translation="MTDQQAEQEIRQRETHRFEAIVRGDVAALEDILSDDLSYTHATG
                     VFETKEEFIAKLRSGQLKY"
     gene            complement(214..1470)
                     /locus_tag="AC16G0_04235"
     CDS             complement(214..1470)
                     /locus_tag="AC16G0_04235"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR5098865.1"
                     /translation="MKPDVTEKLALHGGPKAKRTPFPKGKRHGDLEKRYLGEVIDSDM
                     LFYFMGTKVRAFEKRFAEMYGVKHCIACSSGTSAVHMAVASLQLPLGSEVIIPAITDM
                     GSLTGILYQGLVPVFADVEPDTLNLSPASVRERITERTRALLVVHHSGLAADMDAFLA
                     LGKEAGIPVIEDCAQAYYCSYKGRLAGTMGAINAFSLNHFKHITCGSGGMVLTNDDQL
                     RYRASLFLDKCFQREEGIRNPFFLAPNYQMTELQGAVALAQIEKVQDIVTRRNRLGTA
                     LVAAVREIPGVSCLVTPEGRRHAYFLYLFNLDLKALGCTSAEFSEALNAEGVPNKPRL
                     ITGGRPVYLYDIFQNRSAFPGSTFPFTRPDGSGRVYKEGDCPVAESAFDTWITMNIYE
                     HYTQTDIQEIAHGIAKTAHFFRSRAH"
     gene            1753..2511
                     /locus_tag="AC16G0_04240"
     CDS             1753..2511
                     /locus_tag="AC16G0_04240"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006718301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5098866.1"
                     /translation="MALLEGKVALVTGAAVGIGRAIAAVYGREGAKVVVNYSKSRQEA
                     ERTADLVKQAGGEPLLMQADVAQDGQVRGMVSQILDRLGRIDILVNNAAITAFVEFSN
                     LEGLTDDVWDRLYAVNVKGTFFCCRAVVPAMRKQGHGRIINIASAAGMFAQGSSIAYS
                     CSKAAVIHMSKCLARTLGPKILVNAISPGFIADTRWNEGRPDLEATVQKAAAVPLQRV
                     GKPDDIADAALFLATRGDFMTGDVIVVDGGRLLN"
     gene            2586..3293
                     /locus_tag="AC16G0_04245"
     CDS             2586..3293
                     /locus_tag="AC16G0_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0160254 - tRNA queuosine(34) biosynthetic
                     process from salvaged queuosine or its precursors
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HIR5098867.1"
                     /translation="MPAQTGQPALVDGRYSSYFVLIVAVFITCLITANVTAVKLVEVS
                     GLVVPAAIIIFPISYIIGDVLTEVYGYRRARLVIWLGFLCNLITVGGIWLGQALPAAS
                     FWDGQAAYERILGYTPRILAASFLAYLVGEFANAFVLAKMKIATRGRWLWTRTIGSTL
                     VGQGLDSLVFITLAFLGTIPGSRLLSAIVTQWLAKSVYEAAVTPLTYAVVNFLKRREG
                     MDVYDHGTDFNPLSLTQ"
     gene            3913..>4172
                     /locus_tag="AC16G0_04250"
     CDS             3913..>4172
                     /locus_tag="AC16G0_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098868.1"
                     /translation="MRSTRVLAVFGLALALMAAGAYADSEWLKGSTEEKLKTLADLQP
                     GLGTVMIEYANRYTNAYYAAKGGNWDLAAYQIKEAREIQEVG"
BASE COUNT          698 a         1288 c         1384 g          802 t
ORIGIN      
        1 catacttgag ctgcccggac ctgagcttgg cgatgaactc ctccttggtc tcgaagacgc
       61 cggtcgcgtg cgtgtagctg agatcgtcgg acaggatgtc ttcgagcgcg gcgacatcgc
      121 cccgcacgat ggcctcgaaa cggtgggttt ccctctggcg gatctcctgt tcagcctgct
      181 ggtcggtcat gggttcctcc gtgttccggc cgctcaatgc gcgcggctcc ggaagaaatg
      241 tgccgtcttg gcgatgccgt gggcgatctc ctggatatcg gtctgggtgt agtgctcgta
      301 gatgttcatc gtgatccagg tgtcgaaggc cgactcggcc acggggcagt cgccctcttt
      361 gtagacgcgg ccggacccat ccggccgggt gaaggggaag gtgcttccgg ggaaggccga
      421 gcggttctgg aagatgtcgt acagatacac cggtcgtccg cccgtgatga ggcgaggctt
      481 gttcggcacg ccctcggcgt tcagcgcctc ggagaattcg gcggacgtgc atccaagcgc
      541 cttcaggtcc aagttgaaca ggtaaaggaa gtaagcgtgc ctgcgcccct cgggggtcac
      601 caggcatgag acaccgggga tctccctcac agcggcgacg agtgcggtgc cgaggcgatt
      661 ccggcgggtc acgatgtcct ggaccttctc gatctgcgcc aaggccacgg cgccttgcag
      721 ctcggtcatc tggtagttgg gggccaggaa gaatgggttg cggatgccct cttcccgctg
      781 gaagcatttg tccaggaaca gcgaggcccg gtagcggagc tggtcgtcgt tggtgaggac
      841 catcccgccc gagccgcacg tgatgtgctt gaagtgattc aaggagaagg cgttaatagc
      901 gcccatggtc ccggccagcc tccccttgta ggagcagtag tacgcctggg cgcagtcctc
      961 gatgacgggg atcccggcct ccttgccgag ggctaggaag gcatccatgt ccgcggccag
     1021 gccgctgtgg tggactacca ggagggcccg cgtccgctcc gtgatccgct cccggaccga
     1081 ggccgggctc aggttgaggg tgtccggctc cacatccgcg aacacgggga ccaggccctg
     1141 atacagaatg ccggtgaggg agcccatgtc ggtgatagcc gggatgatca cctcgctgcc
     1201 taagggaagt tggagagacg ccacagccat gtggaccgcc gacgtgccgc tggagcaggc
     1261 gatgcagtgc ttgaccccgt acatctccgc gaaccgcttc tcgaaggcgc gcaccttggt
     1321 tcccatgaag tagaagagca tgtcggagtc gatgacctcg cccaggtagc gcttctccag
     1381 gtccccgtgc cgcttgccct tgggaaacgg ggtccgcttg gctttggggc ctccgtgcaa
     1441 ggccagtttt tcagtcacat cgggtttcat gctgcctcct cgtgccggtg gatgggcgtg
     1501 agagctgatt ccaataccct cgctttcggg ggaacaagta ccacgcctct cggatgaagg
     1561 gcgcgtcggg ggaaagctat cgaatgcctc cctgcctgtc aaccggaaag cctcttcctc
     1621 ttgacaccag ggggaaagaa cgccataaag ccgtgggaag tctcagcggc agctccggga
     1681 gtaggccggt tgtggcgcgg gacaaggctg gtcgagcgaa agatcacgca tctctttcat
     1741 tggaggagaa ccatggcgct tcttgaaggc aaggtggccc tcgtcacggg cgcagccgtc
     1801 ggcatcggtc gggctatcgc cgcagtctac ggacgcgagg gggccaaggt tgtcgtgaac
     1861 tactcgaagt cgcggcagga ggccgagagg acggcggatc tggtcaagca ggcgggcggt
     1921 gagccgctgc tgatgcaggc ggatgtcgcg caggatgggc aggtgcgggg catggtctcg
     1981 cagatcctgg atcgcctcgg gcgcatcgat atcctggtga ataacgccgc catcacggcc
     2041 ttcgtggagt tctcgaatct cgagggattg accgacgacg tctgggaccg cctctacgcc
     2101 gtgaacgtga agggcacctt cttctgctgc cgcgcggtcg ttccggcgat gcgaaagcaa
     2161 ggtcacggcc ggatcatcaa catcgcctcg gctgccggca tgttcgccca gggcagctcc
     2221 atcgcctact cctgctccaa ggccgcggtc atccacatga gcaagtgtct ggcgcgcacc
     2281 ctgggtccca agatcctggt gaacgccatc tcgcccggct tcatcgcgga cacccgctgg
     2341 aatgagggcc ggccggatct cgaggcgacg gtacaaaagg cggcggcggt gcctctccag
     2401 cgagtgggga agcccgacga cattgccgat gcggccctct ttctcgccac gcgcggggac
     2461 ttcatgacgg gggacgtcat agtggtggac ggtggacgtc tcctgaattg aactggattc
     2521 ttcgcccccg aagggcgtcg ccacgcaccc ctccaggtgt cccgcgcctg aaagaggtga
     2581 cacgaatgcc agcgcagact ggacagccgg ccctcgtcga cggccggtac tcatcctatt
     2641 tcgtgctcat cgtcgctgtc ttcatcacgt gtctcatcac cgccaacgtc acggcggtca
     2701 agctggtgga ggtgtccggc ctggtcgtcc cggcggccat catcatcttt ccgatcagct
     2761 acatcatcgg cgacgtcctg accgaggtgt acggctatcg ccgggcgcgg ctggtcatct
     2821 ggctcggctt cctgtgcaac ctcatcaccg tcggagggat ttggctgggg caggccctgc
     2881 cggcggcctc cttctgggat ggtcaggccg cctacgagcg gatcctgggc tacacgcccc
     2941 gcatcctggc tgcctcgttt ctggcgtatc tggtggggga attcgccaac gccttcgtcc
     3001 tggccaagat gaagattgcc acccgcggac gctggctatg gacgcgcacc atcggttcga
     3061 ccctcgtggg ccaggggctg gattcgctgg tcttcatcac actggccttc ctcgggacga
     3121 ttccggggtc gcggctcctg tccgccatcg tcactcaatg gctggcaaaa tcggtctatg
     3181 aggcggccgt gacaccgctg acctacgcgg tggtcaactt tctcaagcgg cgagagggga
     3241 tggatgtgta tgaccacgga acggatttca accccctctc gctgacccag tgaccttccc
     3301 tctccgatcc ctggccacgc gaggaccgtc catttcgttg ccttcccacg ctcaatgcgt
     3361 tagctttgat cacgaagaag ttaccctggg cacgtgagtc ggggtccgtt cggggcacgt
     3421 ccattggtgc ggggatgcca tccttggccg gccagaagag gaaggcgggg gaaagtcacg
     3481 gagccacggt cgttctgacc gtggtcttcg ccgccctcgc ggtgggcctc gtcccaaccc
     3541 ttgctctagc cgaggtgggc gaggaggggg gactcctgga tcgcgctctc gagttctcga
     3601 cgccccctct cggcgtgttt cagctcgtgc ccgccggtcg ggtatcttcc gagccagtaa
     3661 ctcagccgac cgcggcgtct ctgaggccgg cgatgtgtca tgggccggct gcggcgcggc
     3721 ctccacctac ctcctccgca tagtccgtaa cactcaaccg tcttgcctgg tcaccccaac
     3781 gcgcaggagt ctgggagggg tctttccagg ctgcgtcccg ccacgcggct ctgcggagct
     3841 gaaaacgggt gccgggcgct catgcacttg acagtgtgcc ggctctggcg ccggtacggg
     3901 aggggatatc tcatgcgctc cacacgcgtc ctcgcggtct ttggtctggc gctggctctg
     3961 atggcggcgg gcgcctacgc ggattcagag tggctcaagg ggagcacgga ggaaaagctc
     4021 aagacgctgg ccgacctcca gcctggactg ggaacggtca tgatcgagta cgccaaccgg
     4081 tacacgaacg catattatgc cgccaagggc ggcaactggg acctggctgc ctaccaaatc
     4141 aaggaggcgc gggagatcca ggaggtgggc ga
//
LOCUS       DBNGZC010000233         1659 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2660145, whole genome shotgun sequence.
ACCESSION   DBNGZC010000233 DBNGZC010000000
VERSION     DBNGZC010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2660145"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="AC16G0_04255"
     CDS             <1..535
                     /locus_tag="AC16G0_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5098869.1"
                     /translation="LWIAFLFTVACNGSIACTRAAETPVRTSIPTLAAATLPPPMAPV
                     LAVGEPTESLKDIARPSVGGGPGQAVELTGDAVSGAKLFVQYCEACHGQEGKGGVSNP
                     GTDDGVVPSLNPIDDTIPNPDLKTYATNVDLFLQHGSRPEGDAPAKIMPAWGDQGVLT
                     QQQIADLIAYIISLNPP"
     gene            complement(602..691)
                     /locus_tag="AC16G0_04260"
     tRNA            complement(602..691)
                     /locus_tag="AC16G0_04260"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(653..655),aa:Ser,seq:gga)
     gene            complement(711..>1659)
                     /locus_tag="AC16G0_04265"
     CDS             complement(711..>1659)
                     /locus_tag="AC16G0_04265"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR5098870.1"
                     /translation="FVPADANLPFALKTARPDICFNLAEGLGGDAREAQVPALLEMLR
                     IPYTGSRIMANAISLDKTLTKRIWRDRRLPVAPFQEFIVGDESLRPELRFPLFVKPAR
                     EGTGMGVDMNAVVENEAQLHERVDYILSTYRQPALVETFLSGREFTVGMIGRWDSKRY
                     SRHPEWYDEKNGYHVFPVLELDSSRSVTPTVYSQAAKSKSVGEEGAPGYFCPADVPPE
                     LGRKLQALAIKAHQAIKAIDVSRTDIRLNAAGDPVLLEINTLPGLTPNYSDLCLEAAA
                     EGIGYEDLILEILQLGASRWKLLEPRQLYPANRPRSTLW"
BASE COUNT          306 a          527 c          514 g          312 t
ORIGIN      
        1 cctctggatc gccttcctct tcaccgtggc ctgcaacggc tcgattgcat gcacgcgggc
       61 cgcagagacg cccgtacgta cctcgatccc cacccttgcc gccgcgaccc tgccccctcc
      121 catggccccc gtcttggctg tcggtgaacc caccgaaagt ctcaaggata ttgctcgccc
      181 atccgtgggg ggcggccccg gccaggccgt cgaactgacc ggcgacgcgg tgtccggcgc
      241 caagctgttc gtgcaatatt gcgaggcctg ccacggccag gaaggcaaag gcggcgtgtc
      301 gaatcctggc actgatgatg gagttgtgcc ctccctaaac ccgatcgatg acaccatccc
      361 caaccccgac ctcaagacct acgcgaccaa cgtggacctt ttcctccagc acggctccag
      421 gccggagggt gatgctcctg ccaagatcat gcccgcctgg ggagaccagg gggtgctcac
      481 ccaacaacag atcgcggacc tgatcgccta cattatcagc ttgaaccccc cgtaggatat
      541 cgggcaaggt tcccgtgagc tctaacgaaa actctcctgc tgcggcagga gatttcgttt
      601 gtggcggaga gggcgggatt tgaacccgcg aacccttgcg ggttacacgc gctccaggcg
      661 tgcgcgctag accgaactac gcgacctctc cactttaaag cgggaagatt ttaccataga
      721 gtgctgcggg gacgattcgc gggatacagc tggcgtggtt ccaggagctt ccagcggcta
      781 gcgcccagct ggaggatctc caggatcaag tcttcatagc caatgccctc cgcggcggcc
      841 tccaggcaca ggtcactgta attcggggtg agccccggaa gggtgttgat ctcgagcagg
      901 acggggtcac cagccgcgtt caggcgaatg tccgtgcggg agacatcgat cgccttgatg
      961 gcctggtggg ccttgatggc cagcgcctgg agcttgcggc ccagctcggg cgggacgtcg
     1021 gccgggcaga agtagcccgg agctccctct tcacccaccg acttggattt ggctgcctgg
     1081 ctgtagacag tgggcgtcac cgagcgtgag ctatccagct cgaggaccgg gaagacatgg
     1141 tatccgtttt tctcgtcata ccattcgggg tgacgagaat agcgcttcga atcccagcgc
     1201 ccgatcatgc ccacggtgaa ttcgcgccct gaaaggaagg tctcgaccag cgcgggttgc
     1261 cggtaggtgc taagaatgta gtctacccgc tcatgcaatt gcgcctcgtt ctctaccacg
     1321 gcgttcatat cgacgcccat gccggtgcct tcccgggctg gttttacgaa cagggggaag
     1381 cgcaattccg gccgcagcga ttcgtctccg acgatgaact cctggaaggg agcgaccggc
     1441 aaccgccggt cgcgccagat gcgcttggtc agagtcttgt ctagcgaaat tgcattggcc
     1501 atgatgcgcg agccggtata gggaatccgc agcatttcga gcagggcggg cacttgggcc
     1561 tcgcgcgcgt cgccgcccag cccctctgcc aggttgaagc agatgtccgg ccgggccgtt
     1621 ttaagcgcga agggcaggtt ggcatccgcc gggacgaat
//
LOCUS       DBNGZC010000234         3247 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1291764, whole genome shotgun sequence.
ACCESSION   DBNGZC010000234 DBNGZC010000000
VERSION     DBNGZC010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1291764"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="AC16G0_04270"
     CDS             complement(<1..331)
                     /locus_tag="AC16G0_04270"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5098871.1"
                     /translation="MNLAGRTAIVTGGARGIGEAIARTLAGAGARVVIGDVLEEQGAV
                     TARSIQAEGGRATFVKADVSTRAGFDALAGAAIREHGQVDILVNNAGALGIGKLLDTT
                     DDLWDRMF"
     gene            complement(352..1257)
                     /gene="aroE"
                     /locus_tag="AC16G0_04275"
     CDS             complement(352..1257)
                     /gene="aroE"
                     /locus_tag="AC16G0_04275"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR5098872.1"
                     /translation="MDPHATDARHEAGLRLSGRTKVAMSLGYPITQSLSPQVQTTAFR
                     SCGLDWIYIAWGVRPEHLAVTIQALRGAENFAGGNVTAPHKERIVPLLDGLTPAAERL
                     QAVNVILRDQDRLIGDSTDGAGALAAVREAGWDPTGKRVLLLGAGGAARAVGCALAEA
                     GVTSVQVCNRTLSRAESLTGLLQVAGCSEVRAGSLADAPAALRQVDWVVNATSVGLQR
                     DAPSLFDYSLLRAPLRVLDLAFFPQETPFLKHARGQGCRILNGVGMLLHQAALSFERW
                     TGLEAPTALMRETLTAALDQRAQTA"
     gene            complement(1266..2072)
                     /locus_tag="AC16G0_04280"
     CDS             complement(1266..2072)
                     /locus_tag="AC16G0_04280"
                     /EC_number="2.8.3.-"
                     /EC_number="4.1.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HIR5098873.1"
                     /translation="MTTDYTREELMACAVSRELRDGQLGFIGLGTGGRAFIYAVGIPS
                     VAIELAHRRGIDFIAQYGVKLEPRIPEAPACFTDADLLAWPCRAMILVEDCLDMFRRG
                     MMDWGFVSGAQIDPYGNLNSVCIGDHAHPKVRLVGAIAQSDHCAHARHTMIIIPHDRR
                     VFVPKVDFISGLGFGEGPGTRERYHHHGGGPGKVVTNLAILDFHPETKRMQVQSLHPG
                     VSRQQVEENTGFALEFPDPLAVTPAPTAEEVGWIRNEIDPRGLWLQAKVQ"
     gene            complement(2069..2965)
                     /locus_tag="AC16G0_04285"
     CDS             complement(2069..2965)
                     /locus_tag="AC16G0_04285"
                     /inference="COORDINATES: protein motif:HMM:NF013323.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR5098874.1"
                     /translation="MREKVLGLREAVALIQDGDTVAIGGHTLRRHPMALLHEIVRQGR
                     RGLKLQGWNNGIDMDLLIGAGCAASVETSYIGISAFGLALNFRRACEKGGLRVIEHSE
                     TTGLDMFRATVMGMSFFPTKTPLGTDLMRTNPNLTEMACPFTGEPFAAVRAARPDVAV
                     IHMHTADRYGNVQLDAERWNDQSADVLIAKAARTTIVSVEQIVSDEEVYRNSLLTVLP
                     RQFVAAVVETPYGAHPCACDARYDYDLEAVGAYYEASKSDQGFTTYLDATIRGTADHF
                     AYLERIGIERLMHLTRTRVARP"
     gene            complement(2984..>3247)
                     /locus_tag="AC16G0_04290"
     CDS             complement(2984..>3247)
                     /locus_tag="AC16G0_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HIR5098875.1"
                     /translation="RERAGLRGEGPVRVITDLATLDFDPETKRMRLASVHPGVTVDEV
                     EQQTGFPLRRAPGFGEMPVPSPAEVRLLREQIDPDGIYIGAKG"
BASE COUNT          517 a         1187 c         1010 g          533 t
ORIGIN      
        1 cgaacatccg gtcccagagg tcatccgtgg tgtccaagag cttcccgata ccgagagccc
       61 ccgcgttgtt cacgaggata tccacctgcc cgtgctcccg gatggccgcg cctgccaagg
      121 cgtcgaagcc tgcccgggtg gacacatctg ctttcacgaa tgttgcccgt cctccctcgg
      181 cctggatcga cctggcggta actgcaccct gctcctccag cacgtcgccg atcaccacgc
      241 gggcgccggc acccgccagc gtgcgtgcga tggcctcgcc gattccccgt gccccgccgg
      301 tcacgatcgc cgtcctgccc gcgagattca ttcctccctc cagtctgtac ttcaggcggt
      361 ctgcgcccgc tgatccaggg ccgccgtcag cgtctcccgc atcagcgccg tcggtgcttc
      421 aagaccggtc cagcgctcaa agctgagcgc ggcttgatgg agcagcatgc ccacaccatt
      481 caggatccta cacccctgtc ctcgcgcatg cttgaggaac ggcgtttcct gcggaaagaa
      541 cgccagatcc aagacccgga gcggggcgcg caggagcgag taatcgaaca gcgacggcgc
      601 gtccctctgg aggcccaccg aggtggcgtt caccacccaa tccacttggc gcagggccgc
      661 gggggcgtcg gccagtgaac cggcccggac ctcggagcat cctgcgacct gcagcaaacc
      721 ggtcagcgat tcggcccgcg agagcgtccg gttgcagacc tggacactcg tcaccccggc
      781 ctccgccaag gcgcatccca ccgctcgcgc cgccccccca gccccgagca gcaggactcg
      841 ctttcccgtg ggatcccagc ccgcctcccg caccgcggcc agagcgcccg ccccgtccgt
      901 gctgtccccg atcagccgat cctggtcccg caggatcacg ttgaccgcct gcaagcgctc
      961 cgccgccggt gtcaggccgt ccagcagggg aacgatgcgc tccttgtgcg gcgccgtcac
     1021 gttgccgccg gcaaaattct ccgctccccg cagggcctgg atcgtgaccg ccaggtgctc
     1081 cggccgaacc ccccaggcaa tgtagatcca atccagaccg caggaccgaa aggccgtcgt
     1141 ttgcacctga ggcgacaggc tctgcgtgat gggatatccc agggacatcg cgaccttggt
     1201 ccgcccgctc aaccgcagtc ctgcctcatg gcgggcatct gtcgcgtgag gatccatatg
     1261 gccccctact ggaccttggc ctgaagccac aggcctcggg gatcgatctc gttccggatc
     1321 cagcccacct cctctgccgt cggcgccggg gtcacggcga ggggatcggg gaactccaag
     1381 gcgaacccgg tattctcctc gacctgctgt cgcgagacgc cggggtggag tgactggacc
     1441 tgcatccgct tcgtctccgg atgaaaatcc aggatggcca ggttggtcac caccttgccc
     1501 ggcccgcccc cgtggtggtg gtatcgctcg cgggtccccg ggccctcgcc gaaccccaga
     1561 cccgagatga aatcgacctt gggcacgaac acccgccgat cgtgggggat gatgatcatg
     1621 gtgtgccgcg cgtgcgcgca gtgatcgctc tgggcgatcg cccccaccag gcgcaccttg
     1681 ggatgggcgt gatcgccgat gcagacgcta ttcaggttgc cgtacgggtc gatctgagcc
     1741 cccgagacga acccccagtc catcatcccg cgccggaaca tgtcgaggca gtcctcaact
     1801 aggatcatgg cgcggcacgg ccaggccaag agatcggcat ccgtgaagca ggcgggcgcc
     1861 tccgggatgc gcggctcgag cttcaccccg tactgggcga tgaaatcgat cccgcgccgg
     1921 tgcgccagct caatcgccac gctggggatg ccgacggcgt agatgaacgc gcgacccccc
     1981 gtgcccaggc cgatgaatcc cagctggccg tcccgcagct cccgtgagac ggcgcaggcc
     2041 atcaattcct ctcgcgtgta gtcggtcgtc atggtcgcgc cacccttgtc cgcgtgaggt
     2101 gcatcagccg ctcgatcccg atccgctcca ggtacgcgaa gtgatcggcg gtgccccgga
     2161 tggtcgcatc caggtacgtc gtaaaaccct ggtcggattt cgacgcctcg tagtacgccc
     2221 ccaccgcctc caggtcgtag tcgtagcgcg cgtcacaggc gcaggggtgg gcgccgtagg
     2281 gggtctccac cacggcggcg acgaattgac gggggagaac cgtcagcagt gaattccggt
     2341 acacctcctc gtcgctcacg atctgctcca cgctgacgat ggtcgtccgg gccgccttgg
     2401 cgatcaagac gtccgcgctc tgatcattcc atcgctccgc gtccaactgc acgttcccgt
     2461 accggtcggc ggtgtgcatg tggatcacgg ccacatccgg ccgggccgcc cggaccgccg
     2521 cgaagggctc cccggtgaac ggacaggcca tctcggtgag gttcggattg gtccgcatga
     2581 ggtcggtccc gagaggcgtt ttcgtgggaa agaagctcat ccccatcacc gtggcccgga
     2641 acatgtccag gccggtggtc tcggaatgtt cgatcacgcg gagcccgccc ttctcgcagg
     2701 cgcgccggaa attcagcgcc aggccgaacg cggagatccc gatgtacgag gtctcgaccg
     2761 aggccgcgca gccggcgcct atgagcagat ccatgtcgat accattgttc cagccctgca
     2821 gcttgagacc ccggcgacct tgtcggacga tctcgtggag cagggccatg gggtggcgcc
     2881 gcagggtgtg tcccccgatc gccaccgtgt cgccgtcctg gatcagggca accgcctccc
     2941 gcaaccccaa gaccttctcg cgcacgcagt ccacctctcc gtctcagccc ttcgccccga
     3001 tgtagatgcc gtccgggtcg atctgttcgc ggagcagccg cacctcggcg gggctgggca
     3061 ccggcatctc cccgaatccc ggcgcccgcc gcaggggaaa gcccgtctgc tgctccacct
     3121 cgtccaccgt cacgccgggg tgcacgctgg ccagacgcat ccgtttggtc tccgggtcga
     3181 agtcgagggt ggccaggtcg gtgatgaccc ggaccgggcc ctcgccgcgc aagccggccc
     3241 gctcacg
//
LOCUS       DBNGZC010000235         2014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1193762, whole genome shotgun sequence.
ACCESSION   DBNGZC010000235 DBNGZC010000000
VERSION     DBNGZC010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1193762"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1184
                     /locus_tag="AC16G0_04295"
     CDS             174..1184
                     /locus_tag="AC16G0_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5098876.1"
                     /translation="MLTRPGSRNRAIYAAPAAALLLLLVLPGFIQDEYYLHVLVGILF
                     FAYMASAWNIVCGYTGQLSLGHSALCGIGGYISTLLFINLGLTPWVGMLIGGLCATVV
                     GVLIGYPCFRLRGPYFALTTIAFAEILRIWTENTEEILGIELRGAQGISVPLKGHRPW
                     LFQFGDKEPYYYIILGMLLLVLAITYWMERSRVGFYLKAIRGDQDAAEALGINSTRHL
                     LTAMALSSFLTALGGSFYAQYFRYINPERNMGLDLSIEFALMGIVGGQGTVLGPVLGA
                     FLLTPAGEISRATLGGRFPGLHLLIYGLVLVVAVLFLPKGLIHPFRQFLGRRGRADTE
                     SK"
     gene            1374..>2014
                     /locus_tag="AC16G0_04300"
     CDS             1374..>2014
                     /locus_tag="AC16G0_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5098877.1"
                     /translation="MTLLEVDGISKAFGGLQAVKGLSFTLESGEIFGIIGPNGAGKTT
                     AFNLIAGYFTPDSGRIRFKGEDITGLRPWDISHRKIARTFQLSKPFGDLSVLENVMVG
                     GFHHQGSRPAAWRKAETVLAFIGMSHQTDIEAHNLTATDRKRLELARCLATDPELLLL
                     DEVVAGATPAEAVAMMELIRKIRDSGVTILMVEHVMRVVMGLSHRVLVLHHGET"
BASE COUNT          329 a          655 c          619 g          411 t
ORIGIN      
        1 cgggtcgatc ctggggggcc tcatcctcgg ggtggtagag tcggtgaccg cccagttcgt
       61 gaccgccacc tcggcgtcga tcttttcctt cgtcatcttt atcctggtgc tgttcttccg
      121 acccacaggt ctcttgggga tgaaggtgtg acgcccgtca ggacaacgga gccatgctga
      181 cacggcccgg atcccgtaac cgcgcgatct acgcggcgcc cgcggctgcg ctgctcctgt
      241 tgctcgtcct gcccggcttc atccaggacg aatactacct ccatgtcctg gtcggcatcc
      301 tgttctttgc ctacatggcc tcggcctgga acatcgtctg cgggtacacg ggacagctct
      361 cgctcggtca ttcggcgctg tgtgggatcg ggggctacat ctcgaccctc ctcttcatca
      421 atctcggcct caccccctgg gtcggcatgc tgatcggcgg gctatgcgca acggtggtgg
      481 gggtcctcat cgggtaccca tgcttccggc tgcggggccc gtacttcgcc ctgacgacca
      541 tcgccttcgc cgagatcctc cgcatctgga cggagaacac cgaagagatc ctggggatcg
      601 aactccgtgg cgcgcaaggg atcagcgttc ccctgaaagg gcaccgcccc tggttgttcc
      661 agttcgggga caaggagcct tactattaca tcatcctcgg aatgctgctc ctggttttgg
      721 ccatcacgta ctggatggag cgatcccggg tgggcttcta cctcaaggcc atccggggcg
      781 accaggacgc cgccgaggcc ttgggaatca actcgacgcg tcatctgctc acggccatgg
      841 ccctgagctc gtttctcacg gcgctggggg gcagcttcta cgcgcagtat tttcgctaca
      901 tcaaccccga gcggaacatg ggcctggatc tctccatcga gttcgccctc atggggatcg
      961 tgggggggca ggggacggtg ctcggtcccg tcctcggtgc tttcctcctg accccggcgg
     1021 gggaaatctc ccgggccacc ctgggaggga ggttcccggg actccacctg ttgatatacg
     1081 ggctggtcct ggtggtggcc gtgctgttcc tccccaaggg actcatccac ccgttccgtc
     1141 agttcctggg acggcgcggg cgggcggaca ctgagtccaa atgatttaac ctcaccgcag
     1201 aggacgcaga gaatgaacca tctcggaata aacgtccccg tggcaagccg cagcgtagcg
     1261 ttttctgttc ccgcccgtca ttccgtgcgt gacacggaat ccagtcgggc ttgccgtctc
     1321 cctctgcgct ctttgcgatc tctccggtaa gtaattcaga tgaaggatgc ccgatgacac
     1381 tcctcgaggt ggacggcatc agcaaggcct tcggcggcct ccaggcggtg aagggtctca
     1441 gcttcaccct ggaatcgggg gagatcttcg gcatcatcgg tcccaacggc gccgggaaga
     1501 cgacggcctt caacctgatt gccggctact tcacgcccga ctcgggcagg attcgcttca
     1561 agggcgagga tatcaccggg ttacggccct gggacatctc gcatcggaag atcgcccgga
     1621 cctttcagct ctccaagccc ttcggggacc tgagtgtcct ggagaacgtc atggttggcg
     1681 gattccacca tcagggcagc cggccggcgg cttggcggaa ggccgagacg gttcttgcct
     1741 tcatcggcat gtcccaccag accgatatag aagcccacaa cctgaccgcc accgaccgga
     1801 agcgcctgga actcgcccgc tgcctggcca cggatccgga gcttctcctg ctggacgagg
     1861 tggtggccgg ggccacgcct gccgaggccg tggccatgat ggagctcatc cggaagatac
     1921 gtgacagcgg cgtcaccatc ctcatggtgg aacacgtcat gcgcgtcgtc atggggctca
     1981 gccatcgcgt cctggtgctc caccacgggg agac
//
LOCUS       DBNGZC010000236         1254 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1975760, whole genome shotgun sequence.
ACCESSION   DBNGZC010000236 DBNGZC010000000
VERSION     DBNGZC010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1254)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1975760"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..>1254
                     /locus_tag="AC16G0_04305"
     CDS             18..>1254
                     /locus_tag="AC16G0_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR5098878.1"
                     /translation="MIQSGLALRRETVDEEQLKRAALPALSRLVDEIGVSSLLLAPLR
                     GSHATVGVMGLARHRGDRAFDEADETLLVEMAYQLGLALEGQQLIASLRADIAAFLST
                     QQALMVSEEWFRSVFETTTLGIEMMDPVGTILDTNAAFEAMTGYSRSELVRTRYEAIQ
                     HPDDVIPVRQILTAIQMSGEAGAPFENRLLHRNGTTVWVRTNFAGVKKSPDDETAGLV
                     VALHEDITGRRQTEQYFQGVLEAIPDALVLVDAQGRLLLVNRQMEQMFGYERQEILGQ
                     NVEQLIPDRLRDRHPLHRAEYMLSPHIRPMGANLDLFAVRKDGTEFPVEISLSPLQVD
                     EVPVVVAAIRDISPRKQREAALLRSQHSLAEAQAVAHLGSLDYDIASGKLEASDEALR
                     IFGIPREEFQGSETVRARMH"
BASE COUNT          232 a          393 c          421 g          208 t
ORIGIN      
        1 cggggtggat cggacgcgtg atccagagcg gcctggctct ccgccgcgag acggtggatg
       61 aagaacagct caagcgggcg gcgctgccgg ccctttcccg gcttgtggat gagatcggcg
      121 tttcaagcct attgctcgca ccactccggg gctcccacgc gaccgttggc gtcatgggcc
      181 ttgcccggca ccgcggcgat cgggccttcg atgaggccga cgagacattg ctggtggaga
      241 tggcatacca gctgggactg gcattggagg gccagcagct gatcgcctcg ctgcgcgccg
      301 atatcgcggc attcctgtcg acccagcagg cgctgatggt cagcgaggag tggttccgct
      361 ccgtgttcga gaccacgaca ctgggcatcg aaatgatgga cccggtcgga accatcctgg
      421 acaccaacgc ggccttcgaa gccatgaccg gctactcgcg gtccgaactt gtccgtacgc
      481 gttacgaggc gattcagcac ccggacgacg tgatcccggt ccgtcaaatc ctgactgcga
      541 tccagatgag cggcgaggcc ggggcgccct ttgagaaccg actgctccac cggaatggca
      601 ctacggtgtg ggtcaggacg aattttgcgg gggtcaagaa gagcccggac gatgagaccg
      661 cgggccttgt ggtcgcgttg catgaggaca tcaccggacg gaggcaaact gagcaatatt
      721 tccagggagt gcttgaagcc atccccgatg cgctcgtcct ggtggacgcg cagggcaggc
      781 tcctgctggt caaccgccaa atggagcaga tgttcggcta tgagcgccag gaaatcctgg
      841 gacagaacgt ggagcagctt atccccgacc ggctgcgcga caggcacccc ctgcaccgcg
      901 ccgagtacat gctgagcccg cacatccgcc ccatgggtgc gaacctcgac ctcttcgccg
      961 tgcgcaagga cggaactgag ttcccggtgg aaatcagcct cagcccgctc caggtggatg
     1021 aggtgccggt ggtcgtggcg gcgatccggg acatcagccc ccgcaagcag cgggaggcag
     1081 ccctcctgcg cagccagcac agcctggcgg aggcgcaggc ggtggctcat ctcggaagcc
     1141 ttgactacga catcgcctcg gggaagctgg aggcttccga cgaggcattg cgcatctttg
     1201 gcatcccgcg cgaagagttc cagggctctg agaccgtgcg agcccggatg cacc
//
LOCUS       DBNGZC010000237         7922 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2074006, whole genome shotgun sequence.
ACCESSION   DBNGZC010000237 DBNGZC010000000
VERSION     DBNGZC010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7922)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7922)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7922
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2074006"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="AC16G0_04310"
     CDS             <1..364
                     /locus_tag="AC16G0_04310"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5098879.1"
                     /translation="AAVFYFSHIFVGSFWQLFVLYGLAGACVGGLLPSLSAMLVENSQ
                     RGDEGGVYGIDNSVTSAGRIVGPVLGAACALWFSPRATFVLTGLIFAGASLLGALTVC
                     RRSPARPLDRPPVASGRS"
     gene            complement(646..897)
                     /locus_tag="AC16G0_04315"
     CDS             complement(646..897)
                     /locus_tag="AC16G0_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098880.1"
                     /translation="MVSETRGKARDLVIRTHEVSPFRLELQTVGSEVRFDLYYQHQFV
                     PDEMDAMLAGPPKAPPRQVAQAHRFLVRDVCSETQHRSR"
     gene            1821..2255
                     /locus_tag="AC16G0_04320"
     CDS             1821..2255
                     /locus_tag="AC16G0_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5098881.1"
                     /translation="MKTVQQILRHKGNAFWSVTPDTMVYEALKLLAEKDVGALLVLEN
                     GRLVGIISERDYARKVILKGKSSLDTPVREIMTGRVMCVRPDQSVEECMAMVTEKRIR
                     HLPVVVDDEVVGVVSIGDLVKASIDEKDFLIKQLENYITGSR"
     gene            2673..3062
                     /locus_tag="AC16G0_04325"
     CDS             2673..3062
                     /locus_tag="AC16G0_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098882.1"
                     /translation="MRKILVVGMVGCLLLTAVPSPVLAGDPHAVRNRWTGAAIGVGAV
                     ALGGLLLDAIYSPARVAAAPPAVVVPPPAVVYSPPPVVYSPPPVVVYPAYPTLVYAPA
                     PIAVYHGWVPPGHWKKWRKHHWKHGWD"
     gene            3594..4754
                     /locus_tag="AC16G0_04330"
     CDS             3594..4754
                     /locus_tag="AC16G0_04330"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF051259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR5098883.1"
                     /translation="MAGNVAIFRVPAQITFGPGAADSVGTEAKRLGGTRAMVVSDPNL
                     QKLGVTDRILDSLAAQGVGATVFTELEFEPSAGSVAPAAAAARDAGCDLVVGLGGGTT
                     LDTAKAVAMLLRNDGLVEDYLGIGLVKQRGVPSILIPTTAGTGAEITPNALFLVPAVR
                     EKKAVVSPHIIPDIAMVDPLLTHTVAATVTAATGMDALSHAVEAYTSQNASPLTDLYA
                     LEAIRLIGFHLRTAVANGKDEAAREGMARASMYAGIAIGNAGTNIVHALAYPLQGQHR
                     VTHGIANSLLLPYGMEFNALSNLPKFAAVAEALGEPIAGLSPRTAAARSAEACRLLSM
                     DVGIPQRLREVGIKPGDLPALADGAMKVTRLLANNPRQVRPEDARAIFEKAL"
     gene            complement(4810..4992)
                     /locus_tag="AC16G0_04335"
     CDS             complement(4810..4992)
                     /locus_tag="AC16G0_04335"
                     /inference="COORDINATES: protein motif:HMM:NF027851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HIR5098884.1"
                     /translation="MAKMRLDTFVYTDVPADRLVIINGQKYVEGDQVDGLYRIESITR
                     EGALLSYKDEKILLRP"
     gene            complement(5716..7326)
                     /locus_tag="AC16G0_04340"
     CDS             complement(5716..7326)
                     /locus_tag="AC16G0_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004582470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeA family protein"
                     /protein_id="HIR5098885.1"
                     /translation="MSERHREGLAHLLYGIQQPGGFVQLTGEVGTGKTTLCRCLLEQL
                     PPKVDVALILNPRLTAVEFLATVCDELRITYPPETWSIKGLVDGLYSYLLDAHAQGRR
                     TVLIIDEAQNLRPDVLEQIRLLTNLETATLKLLQIILIGQPELIRILEREKLRQLAQR
                     ITARYHLLHFSRRDTPAYIRHRLRVAGGSDTLFTPAAMRCVHRLSGGVPRVINVICDR
                     ALLGAYAHDQHRVTAGMVRRASREVRGAVSWKRRGGRLGWTLGLGALAVAAAGTTILV
                     TPNRLSLPRDRGAPAVLSTDARSTAVASVPGESQRAPEGGPSGSVDRGPTAPRLADLL
                     ADASLGRDDNAAFASLYSRWGLEPRKTRTGLGCESGRSQGVECLFNVGSWNKLRRFDL
                     PAILELALPGGERRRVALVALGDNSATLALGGREYTFPLSEVERFWDGPFILLWKVPP
                     LESRLISPGMRGKDVEWLRQRLDEIEGKPSKGARSNLYDQELKQRVLAFQASRSLSPD
                     GLVGADTLAQLTLAAREPGTPSLSRRAP"
BASE COUNT         1401 a         2448 c         2627 g         1446 t
ORIGIN      
        1 ggcagcggtt ttctacttct cccacatctt cgtgggaagc ttctggcaac tgttcgtgct
       61 ctacggtttg gcgggcgcct gcgtgggagg cttgctcccg tccttgtccg ccatgcttgt
      121 ggagaactcc cagcggggcg acgagggcgg ggtgtacggg atcgacaact cagtgacgtc
      181 ggccggccgg atcgtgggcc cggtgctggg cgcggcctgc gccctctggt tctcgccccg
      241 ggcgaccttc gtcctcacgg gcctgatctt tgccggggcc tcgctcctcg gtgcgctcac
      301 cgtctgccgc cgcagtccgg cgcgacccct cgaccgtcca cccgtggcca gcgggcgttc
      361 ctgacgctcc agtgcaactc cgcggcctca tcactggcga tcagcctcga gggatccagc
      421 caggggtctg gattcggcgg cgggagattc acgagccccg gctcgggatt gccctgcctt
      481 atcctggacc cgaaccgcgg aacgggatct cgcgaccgtg gtcttatact cttctcaatc
      541 ctcgaagttg cactcgatca ggtgggaggg tgtgattgct ggcggggcgt tcagccgaac
      601 cggaatcaag gtgcaggcgc cggactcctc cctgcgcctc gagcctcagc gggatcgatg
      661 ctgcgtttcg gaacagacgt cgcggaccag gaatctgtgc gcctgggcca cctggcgcgg
      721 tggtgccttc ggcggtccgg ccagcatggc atccatctca tcgggcacaa actggtgctg
      781 gtaatagagg tcgaatcgta cctcgctgcc aaccgtctgc aactccaacc ggaagggaga
      841 aacctcgtgt gtccggatca ccagatcccg agccttccct cttgtctctg agaccatgcg
      901 atccctcaag tccaccccga ccagctcaaa ttccagaaat cgtacctctg cccacggggt
      961 ctgggcgatc ccatcgaatt ggagccggcc cgcctccggc cgcacacagt tccagtatag
     1021 gaccacatgg gaactaccga tcctatgggc aaaggtactc agcgagaccg gggatgtcgt
     1081 cgccggcgtt tgcaccgaca cacaacccat caaacaaaat acgaggggta cagccagcca
     1141 ggacctcgag aacatggtct ccctcctgta tgcctgtcgt gtctccgcga tttgcagggc
     1201 cgctgaaccc cgcgccgccc cgtgggtttc acccgggaag cccgaccagc ggggtcgatt
     1261 cttccccttg gatttggacc aagtgaggcc acccgcgaaa cggggagatg ccgggagatc
     1321 ggctaggagc gtatctctgg catgcacccg agtcaaccgg aatccggatg tcggtcggca
     1381 tcgggttcac cgctgccggg caggatcctt cggcccgggg cgaccgtcag gccgcccatc
     1441 gggctgaaaa ggtgtctacg gacttccgat tgaccacact actatgatct tgacaatact
     1501 ctccaccccg ggtagcttct cgtcacttcg atgacgtgcg atagggatgg ggagggggca
     1561 gtgcgagggg gcaacgagcc accatatcat cacatgaaaa tggttccctt tcgggcctag
     1621 agcaagtaac gaccctttgg ggatgcgtga cccccaaagg gctttgcttt tggaagcacc
     1681 cacccagatc gcatttgttg actcccaagg gcatcccgat ccgcgtcgat tcttcggtat
     1741 cagggcgacc ccgagggcaa ccctctttgg tggctgccct gtcgtttcca gcgactgaac
     1801 cattccggcg gagggtgggc atgaaaaccg ttcaacaaat cctgcgacac aaagggaacg
     1861 ccttctggtc cgtgacaccg gataccatgg tttacgaggc cctcaagcta ctggctgaga
     1921 aagatgtggg ggcactcttg gtcttggaga acgggaggct ggtgggcatc atctcggagc
     1981 gggactatgc caggaaggtg atcctgaagg gcaaatcctc cctggatacc cctgtcagag
     2041 agatcatgac cggccgggtc atgtgtgtgc gtccagatca gtcggtcgaa gaatgcatgg
     2101 ccatggtcac ggagaagcgg atccgccatc ttcccgtcgt cgtcgatgac gaggtcgtag
     2161 gtgtggtgtc catcggggat ctcgtcaagg cgagcattga cgaaaaggat ttcctgatca
     2221 agcagctcga gaattacatc accggaagcc gataggggat gtgtcgggtt ctgtaatccc
     2281 tgtcgttctt gtgattcggc ccgaagagtt tcggcgccaa gatcggcagc ccctttggcc
     2341 aagtgctcgg tgagatctgg tgtatcggca ccgtatgctc tgacccctgc cagactcctc
     2401 tgtgggatag atttacaatt tcgggtagaa tctacacgct agcggacaac gatcaagccc
     2461 cagccttgac ccctgtctcg cctttccaag gctgcaatcg cttatttccg aagaccttgg
     2521 acgcgaggac tgctgttagt tgcgtgctgg cattcaattt gctgaaacac caagtcaacg
     2581 accgcagagg ggaaccgggg atattggcat cccgtcttcc tcgagaatga gagtatggct
     2641 tccacgcggg actggtcagg gaggtgctcc tagtgcgtaa gatcctggtg gttggtatgg
     2701 tgggctgctt acttctgact gccgtcccat ccccggtcct ggcgggggat cctcacgctg
     2761 tgcgaaaccg ctggacgggt gccgccatcg gcgtcggggc cgtcgccctc ggcgggctcc
     2821 tcctggacgc catctatagt cccgctcgag tcgccgcggc ccctcccgca gtggttgtgc
     2881 ctcctccagc tgtcgtttac tccccgcccc ccgtggtcta ttcccccccg ccagtggtgg
     2941 tctatccggc ttatccaacc ttggtgtatg cgccggcccc gattgcggtc taccacggat
     3001 gggttccacc cggccactgg aagaagtggc gaaagcatca ctggaaacat ggctgggatt
     3061 agcaggctgg gtatcagagg gatccagcca agcgagcata gagcttctga agaagacccg
     3121 gagctgtgac gcgagcggga tgggctgcag ctccggtgat gcaaggggag cccgagcgat
     3181 cttcgaagaa cagccagcgc aagtccgtgc ttgaggacca tccggctcct gcggcgcacg
     3241 gtccctgtgg tggcattctc ccttcgtatc ctccgccgag aatttctccc tagttcctgt
     3301 cagcattcaa tcaacgtcca agctgttcgg aagcttccca gcccattcag gctcctgacc
     3361 accaaaccga tcatagtgcc tggtctccct tgacatgcgt cagcgtctgc gcgaccatgc
     3421 agccccatct gtgatatctg agggctatcg agacagtcac gaggggaagg cgggaggcgt
     3481 gggtcaactc gagtccacga ccgcaaggta tccgggcaaa tccacgggcg gtccgtgtgt
     3541 cgcccagcag cgtgaatagc tacggcccct ctcggggcag agggaggaag ctcatggcag
     3601 ggaatgtggc aatcttcaga gtgccagcgc agatcacctt cggccccggc gctgccgaca
     3661 gcgtggggac ggaggccaag cggctcggag ggactcgcgc gatggtggtg agcgacccca
     3721 acctccagaa gctcggcgtg accgaccgaa tcctcgactc gcttgcggcg cagggggtgg
     3781 gggcgaccgt gtttaccgag ttggagttcg agccgtcagc cggaagcgtc gcgcccgccg
     3841 cagccgcggc aagagacgca ggctgtgacc tggtcgtggg actggggggc ggcaccaccc
     3901 tcgacaccgc caaggctgtg gcgatgctgc tgcgaaacga tggcctggtg gaggactatc
     3961 ttgggatcgg gctggtgaag cagcgcgggg tcccgtccat cctcattccc accaccgccg
     4021 gcacgggcgc cgagatcacg cccaatgccc tctttctggt ccctgcggtc cgggagaaaa
     4081 aggccgtggt aagtccgcac atcatccccg acatcgccat ggtggatccg ctcctgaccc
     4141 atacggtcgc ggcgacggtg acggcggcga cgggaatgga tgccctgagc catgcggtgg
     4201 aggcctacac ctcccagaac gcctcacccc tgacagatct gtacgcactg gaagccatcc
     4261 ggctgatcgg cttccacctg cgtacggccg tggccaacgg caaggacgag gcggcgcggg
     4321 agggcatggc cagggcaagc atgtacgcgg ggatcgccat cggcaacgcc ggcaccaaca
     4381 tcgtgcatgc gctggcctat cctctccagg ggcagcaccg ggtgacgcac gggatcgcga
     4441 actccctgct cctgccgtac ggcatggaat tcaacgcgct gagcaacctt ccaaagtttg
     4501 ccgccgtggc cgaggccttg ggcgagccga tcgccggcct ctctccccgc accgctgcgg
     4561 cgcggagcgc ggaggcctgt cggctgctgt ccatggacgt gggcatcccg cagcggctgc
     4621 gcgaggtggg gatcaagccc ggggacctgc cggcgctggc ggacggcgcg atgaaggtga
     4681 ctcgcctctt ggccaacaat ccacggcaag tccgccccga ggacgcccgc gccatcttcg
     4741 aaaaggcgct gtaaatttgt tgctgggggc atgccgcagg ggtccaatga cggtgggggg
     4801 acgcagaatt taggggcgaa ggagaatctt ttcgtccttg tagctcaaga gcgccccctc
     4861 gcgggtgatg ctctcgatgc ggtagagtcc gtccacctga tctccctcga cgtacttttg
     4921 tccattgatg atgaccaggc gatctgccgg gacgtccgtg tacacgaagg tatccagcct
     4981 cattttcgcc atggcgtcga ccagcgtcgg cggcgcgacc ggttttgcgg gcaggggtgg
     5041 cggaaccacc actgcagcgg gcggattagc gcctgggctc ggaggcgcct gggggcgcgc
     5101 cggtggctga gccgatccgg gcgccggaac cgtgaagacg ggggccggct ggacaggaac
     5161 gggctcggtt ggtggagtcg ggctcatcgg gggctggcta accgggtttt cagttgaccg
     5221 gcctccgccg cctctcatcc cgccggacga ggcggcggcc agggtatctg acgcggatga
     5281 tggtgtttga ggcacctgga caggacgagg tgtcgtcgcg ggcgcgacgc tttgttgctg
     5341 gggcaccttt ggcggctcca cgactcccgg ccgagatggg acgggtgcga cggaaactgg
     5401 ggggacggtc tcgatggctc gcgcgggtgc gggggtgagc gccgggtttg ccgggcctcc
     5461 tgggagcggg ctgctggcct gaggccgaga gatcgttgta gccggggggg tcgcgggaag
     5521 ggatgggcgc caaagccaga tcgaggcccc gccaccgatg aggagcgccc cgacggccac
     5581 ccagagaccg atgcgccgcc ctgccgtcag gaccgggatg tgcaccgtcg acaacgtcgg
     5641 cactcgggcg aggtcgcgct gccggtcggc ctttttcaat gcatccagga tgaacgacat
     5701 gtcttccgcc cctggctagg gtgcccgacg cgacagcgac ggggtgcccg gctcgcgcgc
     5761 ggccagcgtg agctgggcta gcgtatccgc gccgacgagt ccgtccgggg acaatgatcg
     5821 gctcgcctga aaggcgagga cgcgctgctt cagctcctgg tcatagaggt tggagcgtgc
     5881 accctttgac ggcttgccct cgatctcatc gagtctttgc cggagccatt ccacgtcctt
     5941 gccgcgcatt cccggtgata tcaggcgtga ctcaagggga ggcaccttcc acaataagat
     6001 gaacggaccg tcccagaagc gctcgacctc gctcagcggg aacgtgtact cccgcccgcc
     6061 gagcgccaag gttgcgctgt tgtctcccaa ggccacgagg gccacgcggc gccgctcgcc
     6121 gccgggcaac gccagctcga ggatggcagg cagatcgaaa cgccgcaact tattccaact
     6181 gccgacgttg aagaggcact caactccctg tgaacgtccg gattcgcaac cgagaccggt
     6241 gcgcgtcttc cggggctcca gcccccagcg cgaatacagg ctggcgaagg cggcgttgtc
     6301 gtctctgccc agggaggcgt cggccaggag atcagcaagc cggggggctg tggggcctcg
     6361 gtcgacggag ccgctcggac cgccctcggg cgcccgctgg ctctccccgg gcacggatgc
     6421 gaccgctgtc gagcgtgcat cggtagacag gactgctggg gcgccacggt cccgcgggag
     6481 agagagacga ttcggggtca ccagaatcgt ggtccccgcc gcggcgacag ccagggcacc
     6541 caacccgagc gtccagccca agcggccacc gcgcctcttc caggacacag ccccccgtac
     6601 ttcacgactg gcccgcctca ccatgcccgc agtgacgcgg tgctggtcgt gggcgtaggc
     6661 ccccaggagg gcgcggtcgc agatgacgtt gatcacccga gggacgccgc ccgataggcg
     6721 gtgcacgcag cgcatggctg ccggcgtgaa caaagtgtcg ctcccgccgg ccacgcgcag
     6781 ccgatggcgg atgtaggccg gcgtgtcccg tctggaaaag tgcagcaggt ggtagcgcgc
     6841 ggtgatccgc tgagcgagtt gccggagctt ctcacgctcc aggatgcgga tgagctccgg
     6901 ctgtcctatc aggatgatct gcagcagctt cagggtcgcc gtttcgagat tggtcaggag
     6961 gcgaatctgc tccaggacgt cggggcgaag gttctgcgcc tcatcgatga tgaggacggt
     7021 gcggcggccc tgggcgtggg cgtcgaggag atagctgtac aagccatcca ccagtccctt
     7081 gatgctccac gtctcggggg ggtaggtgat ccgcagctcg tcgcacactg tcgccaggaa
     7141 ctcgacggca gtcagccgag ggttgaggat cagcgccacg tccactttgg gcgggagctg
     7201 ctccagcagg cagcggcaca gggtggtctt gccggttccg acctcgccag tgagctgcac
     7261 gaaaccgccc ggttgttgga tcccgtagag gaggtgggcg agcccctcgc ggtgccgctc
     7321 gctcatgtac aggtatcgcg ggtcgggggt gatcgagaag ggaggttcag tcagtccaaa
     7381 atgcgaggtg tacatcgcgt gttcctgcgt cgggcgccct ccgtgacccg cccaacggcc
     7441 ttcaggtgga ggtggcccgg agacccaggg agattagacc ggagagtgcc ggggcgtcaa
     7501 gggattcgag ccagcacgcc atcggctcga taccactcgg gccatgaccg gacgtgagtc
     7561 cgcctgcgcg gagaatccgc tcgcggcacg ggcaaggccg aacgacgcca tcgtttttca
     7621 agatggcttc ctttgatcgg agtgtcaagg ggaatgtcgc aaggtcgtgc ccggaacctc
     7681 ttgtggtgac attcctaaca atggggagta atcgaggggt atttcaaggt ggccaatccg
     7741 acaactactc gggtaaaaac tgaggcagag gggcagaggg gcttcgcgga tagatattct
     7801 gccttggggg cttttaccct aggacccacg tgccctataa tctcagttga tttgctgcac
     7861 ggaggagctg atcgagagag tggcgaggag gctccgaggg tttcctctcc gggaaaggag
     7921 ta
//
LOCUS       DBNGZC010000238         6478 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2171268, whole genome shotgun sequence.
ACCESSION   DBNGZC010000238 DBNGZC010000000
VERSION     DBNGZC010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6478)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2171268"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="AC16G0_04345"
     CDS             complement(<1..904)
                     /locus_tag="AC16G0_04345"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5098886.1"
                     /translation="MPFPAGLRALNHRDFRIFWTGQGISLLGTWMQSVGQSWLVLELT
                     NSALLLGVVGALQFAPILFFSVFAGAVVDRLPKRRLIIGTQTVLLLQAFILSTLAWTG
                     HVRYWHVAALAWLLGTVNTIDMPARQSFIVELAGTEDLMNAIALNSAVFNGARIVGPA
                     VAGLLVAKYGVALAFFLNGVSFVAVIAALLAIRTEGFPHRRRGTTMRQEIREGIGYAV
                     TTPRVSLVLSVLFVVSMLVINHGTLVPLLARDVLHQGAQGFGFLMAALGAGALSGALV
                     LTLARQGRPPITLVVVPAAVACAAT"
     gene            complement(982..1776)
                     /locus_tag="AC16G0_04350"
     CDS             complement(982..1776)
                     /locus_tag="AC16G0_04350"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR5098887.1"
                     /translation="MEKPVFLTDLTWPEAKARLPRVALAVLPAGSTEQHGPHLTFETD
                     IAIATELARRLAEQHYPRILVAPPLPVGLSNHHMHFHGTITWRAETFTAALTDTIRAL
                     SAHGIPRFLILNGHGGNRGALSVLCTRLRYEEGLLVAACTWFELVRDACQKFIPKRRV
                     HADEIEAAMGLYLVPRVVRRAALRKGGNHPDPYPHTDASTGGAVEVPYFWEELTPNGA
                     YGDARRATAAMGAKLTATFLQRAGEFVAGFMRGSVQPGSRRAGRLR"
     gene            complement(1965..2507)
                     /locus_tag="AC16G0_04355"
     CDS             complement(1965..2507)
                     /locus_tag="AC16G0_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098888.1"
                     /translation="MSPVPAGTPPPAQMPAHPRAQGRGGFEHILEYKAPLPPPTRSIE
                     ETSSLFTWMLDQDPVIPNNLDRLREIHAGVFRDKPAGHLTYEQHILLQVADDLSLHST
                     RESMDLHLKTLAAGYRRAVGAYHIAARCGEHEADSALWQMASLASRLRVEGWIFQTRH
                     GVPPEVAVGVRLRTKRATGD"
     gene            complement(3052..3486)
                     /locus_tag="AC16G0_04360"
     CDS             complement(3052..3486)
                     /locus_tag="AC16G0_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR5098889.1"
                     /translation="MLLAERIVQAAADAIIAADISGNITLWNAGAERLFGFGASETLG
                     RSLDLIIPEAQRKRHWDGYRHVMRTGETKYGTQLLRVPAIKKDGSRFSIAFTVGLLKD
                     GAGVVEGIFAVMRDDSERFQTEKALRKQVAELEKALTQRSGS"
     gene            complement(3529..3927)
                     /locus_tag="AC16G0_04365"
     CDS             complement(3529..3927)
                     /locus_tag="AC16G0_04365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HIR5098890.1"
                     /translation="MAATIPQGFQDLLRKKAFAHLATIMPDGSPQVTPVWFDFDGSHI
                     RVNSAKGRVKDKNMRSRRQVALAIQDPENPYRYLAVRGRVEDVSEAGADAHIDSLAKK
                     YLGQDRYPHRQPGEVRVIYKIRPDRISTMG"
     gene            complement(4027..4389)
                     /locus_tag="AC16G0_04370"
     CDS             complement(4027..4389)
                     /locus_tag="AC16G0_04370"
                     /inference="COORDINATES: protein motif:HMM:NF014655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HIR5098891.1"
                     /translation="MDEWFPRHTFAASGGRPSSALEMLAAGGASGHVAPEEVTDRGTE
                     GGEGSKVFLFNCECHSFEEVITQLLKAVPGMTRALAEDLAWRVHTQGLAEVYRGGASE
                     CQRVAKILGETGLIVQVL"
     gene            4622..5476
                     /locus_tag="AC16G0_04375"
     CDS             4622..5476
                     /locus_tag="AC16G0_04375"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5098892.1"
                     /translation="MDRTNRRWARRELTLAVDGLRLHLGVWGPADALPILLLHGGAAN
                     THWWDWVAPELAGTFRVFALDFPGHGRSARPRPVRYRMADFVAAAAGAVDAVGVRGLH
                     VVGHSMGGKVAMLMAAAHPRRVERLVIVDATPGISAQGLADMRRIGSRPQRLFPSRRL
                     AARAFRLIPPETAAPRARLRALALCSTRHCGHGRWSIGPDREFFSRIVPQVAWPVLTR
                     IRCPTLILRAERSSILSRRTAEAMQSGIPQASLREIPATYHHLLLEQPAEIGRTIRNF
                     LTGEAGLE"
     gene            complement(5543..>6478)
                     /locus_tag="AC16G0_04380"
     CDS             complement(5543..>6478)
                     /locus_tag="AC16G0_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5098893.1"
                     /translation="ANAFLEKPFTPERLLNIVRDLLARKISVRFWGVRGSIPTASRET
                     VRYGGNTPCVEIRISGIDDAFILDGGTGLRELGRSLQRPGTAIHGYLCFSHFHWDHIQ
                     GLPFFEPANTAGNSFTLVGPAQPTANLLQILSGQMASVYFPVGLEQFRAEMVFKEIAE
                     GTYQLGGVQFDTLSSFHPGRSLIYRLSQGGKRIVYATDNELPLGWKATGGDAAHQVDR
                     FIHFFADADLLIHDAQYIPEELAERRGWGHSAWTDVLDLAASAGVKQVILFHHDPDHS
                     DSFLDGIRSSVLQRVSAMGTTTACELAREGDLLVV"
BASE COUNT         1181 a         2131 c         2071 g         1095 t
ORIGIN      
        1 ccgtggcggc gcaggccacg gccgccggca ccaccaccag ggtgatgggc ggacgccctt
       61 gccgcgccag agtcaagacc agggctccag acaacgcgcc cgcacccagg gccgccatca
      121 ggaatccgaa gccctgggcg ccctgatgca gaacatcccg ggcgaggagg ggcacgagcg
      181 tgccgtggtt gatcaccaac atgctcacga cgaagaggac actcagcacc agtgagactc
      241 tgggcgtcgt gacggcgtac ccgatcccct cccggatctc ctggcgcatc gtggtccccc
      301 ggcggcggtg aggaaatcct tccgtccgaa tggccagcag ggcggctatc actgccacga
      361 agctcacccc gttcaggaag aacgccaggg cgacgccgta cttggccacc agcaggcccg
      421 ccacggcggg gccgacgatt cgcgcaccgt tgaacacggc ggagttgagg gcaatggcgt
      481 tcatcaggtc ttccgtcccc gccagctcca cgatgaagga ctggcgagcc ggcatgtcta
      541 tggtgttcac cgtcccgagg agccaggcga gtgcggccac gtgccagtac cggacatgac
      601 cggtccacgc aagggttgag aggatgaagg cctggagcag gagcaccgtc tgggtgccga
      661 tgatgagccg gcgcttgggc agccggtcga cgaccgctcc ggcgaagacg gaaaagaaga
      721 ggatcggggc aaactgcagc gctccgacaa ccccaagcag gagggcggag ttggtaagct
      781 ccaagaccag ccaggattgt cccaccgact gcatccacgt gccgagcagc gagatcccct
      841 gaccggtcca gaaaatccgg aagtcgcgat ggttgagcgc ccgtaagccg gctggaaacg
      901 gcatgcgacc ttccctcgtc tgggaaccac gggaaggcgt acgccttccg ccgacgtcca
      961 tcacgccgac tggaaacaga cctatctgag tcggccagcc ctgcgcgagc caggctgcac
     1021 cgatccccgc atgaaccccg cgacaaattc accggcccgc tggagaaagg tggccgtcaa
     1081 cttcgccccc atggcagcgg tcgcccgccg ggcatcgccg taggcaccgt tcggggtcaa
     1141 ctcctcccag aagtagggaa cctccacggc gcctcccgtg ctcgcatccg tgtggggata
     1201 cgggtcggga tggtttcccc ccttccggag tgccgccctc cgcacaacac gagggacgag
     1261 gtacagcccc atggcggcct cgatctcgtc ggcgtggacc cgtcgcttgg ggatgaattt
     1321 ctggcaggcg tcccggacca gctcaaacca ggtgcaggcc gccaccagga gaccctcctc
     1381 gtaccggagg cgcgtgcaca ggaccgacaa cgccccgcga tttcccccat ggccgttgag
     1441 aatgagaaac cgcgggatcc cgtgagcgga gagggcgcgg atggtgtccg tgagggcggc
     1501 cgtgaaggtc tcagccctcc aggtgatggt cccgtggaaa tgcatgtggt ggttggagag
     1561 tccgaccggc agcggcggcg ccaccagaat gcgagggtaa tgttgctcag ccagcctgcg
     1621 tgccaactcg gtggctatgg cgatatctgt ttcgaaggtc aggtggggcc catgctgctc
     1681 cgtcgagccc gcggggagga cggccagcgc caccctcggc agccgcgctt tggcttccgg
     1741 ccaggtcagg tccgtcagga acaccggttt ctccatcctc gcctccggga tttcgggaca
     1801 cgtcatcatc cctcatcgtc catcgatcac acagacttca agccagggag catctaggga
     1861 ggatctgcgg aatccgtggt gaagcgatcg ttgccctatt cggtgggtcc gggcgttgtc
     1921 cgccaggaca acgatggtgg aatgaacctc cgaagccacg actctcagtc gcctgtcgcc
     1981 ctcttggtcc ggagccttac gcccaccgcc acctccggcg ggacgccgtg tcgggtctgg
     2041 aagatccaac cctccacccg caaccggctt gccaggctcg ccatctgcca gagggcgctg
     2101 tccgcctcgt gttctccgca ccgcgcggcg atgtgatacg ccccgactgc ccggcggtag
     2161 ccagcagcca gagtcttcag gtgaagatcc atgctctcgc gcgttgaatg caagctgagg
     2221 tcgtcggcaa cttgaagaag gatatgctgc tcgtacgtga gatggcctgc aggcttgtcg
     2281 cggaagacac cggcatggat ctctcggagt cgatcgagat tgttcgggat gaccggatcc
     2341 tggtccagca tccaggtgaa cagggaagaa gtctcttcga tggaacgggt cggcggcggc
     2401 aacggcgcct tgtactccag gatgtgctcg aacccgcctc gtccctgcgc tcgcgggtgg
     2461 gcgggcatct gggccggggg tggcgtgccc gcaggaacgg ggctcatcgg catcacgggc
     2521 gactcccccg gtggaaaagg attgagcagg ggagcgtcgg gtgaaatcag cgactgtacc
     2581 ggaccattcg caaccggcgt ctctgcggag gatacagcca cagcgacgga taattccggt
     2641 gcaaaagcct gcgctgcaac aggagactcg gggggctgga gaggtccggg tgataccggc
     2701 ccgccaggcg gcgtgggcag cggggagacc gaaacccggg ccgactctcg aacgaccgga
     2761 ccggtgtcgg gatcctcgac ctgcaatttt cgaagcaaat agacgtccga cggtatccgg
     2821 ccgccctgga gatgctcccg aagatcggcg gcgccctcgg gccggaacac catataacag
     2881 gagggacaaa acgtggcttc ggccgcgacc ttctgctcgc aggccggaca ggctcgaccc
     2941 ttatcgtttc cccgcccgct gaagagccga atcggcattc ctgcctcgat agacctccct
     3001 ggatacagta ttcgcgccgg accataccac catatgtggg ggcatgtcaa atcatgaacc
     3061 actccgctgt gttaaggcct tctcgagttc ggcgacttgc ttccgcagag ccttttctgt
     3121 ctggaaccgc tcgctgtcat ccctcatgac ggcaaagatc ccttccacga ccccggcccc
     3181 gtccttgagc aggcccacgg tgaacgcgat ggagaaccgg ctgccgtcct tcttgatcgc
     3241 cgggacccgc agaagctgtg tcccgtactt tgtctcgccg gttcgcatca cgtgccgata
     3301 cccatcccag tgacgcttcc gttgggcctc agggatgatg agatccagcg accggcccag
     3361 ggtctccgac gctccaaagc cgaacagccg ctccgccccg gcattccaca acgtgatatt
     3421 cccggaaata tccgccgcga tgatggcatc tgcggcggcc tggactatac gttcggcaag
     3481 cagcatgtga tcccgccctc cgtccgccat cgaagttcca ggatctatct accccatggt
     3541 ggagatgcgg tcgggacgaa tcttgtagat gacgcggacc tcgcccggtt ggcgatgcgg
     3601 gtaccggtcc tgacccaggt acttcttcgc caacgaatcg atgtgggcat ccgccccggc
     3661 ttcgctgacg tcctcgactc gccctcggac ggctaggtag cgataaggat tctcgggatc
     3721 ttggatcgcc agcgcgacct gtcgtcggga ccgcatgttc ttgtccttga cccgtccctt
     3781 tgccgagttc acccggatat gactcccgtc gaaatcgaac cagacgggcg tcacctgggg
     3841 actgccgtcg ggcatgatgg tcgccagatg cgcgaaggcc tttttccgga gcagatcctg
     3901 gaatccttgc ggaatggtcg cagccataac ttcctccctt cctcggacca ctgcaccgcc
     3961 acgggataaa ggacaacgac gaaccgacta taccaggcta gcggatggga cgccaggtcg
     4021 tgccgttcac aacacttgca cgatgaggcc ggtttccccg aggatcttgg cgacccgctg
     4081 gcattccgag gcccctcccc ggtatacctc ggccagcccc tgggtgtgta cccgccaggc
     4141 cagatcttcc gccagagcgc gggtcatacc gggaaccgcc ttgaggagtt gcgtgatgac
     4201 ctcttcgaaa ctgtgacact cgcagttgaa gaggaagacc ttgctcccct caccgccttc
     4261 ggttccgcgg tccgtgactt cctcgggcgc cacgtgtccc gacgccccac cagcggccag
     4321 catttccagc gccgaggagg gcctgccacc gctcgctgcg aacgtgtgcc tggggaacca
     4381 ttcatccatc ttcatgacct cccgcgtgga tgaatctatc ccgctgagga aagatgcgca
     4441 agccccggca ggggacagaa ccacgctcgc tgccgggacg ccgcataact gctccacccc
     4501 accgacggtt ccatcctccg acgggccaac ccatgcgccg gctccgttcg tcctgagttg
     4561 gaatatgcta tgatgccgac attcgagaag ccgaaacacg ctcatgggat gggagggctg
     4621 catggatcga accaaccggc gatgggcgcg ccgggagctc acactcgccg ttgacggcct
     4681 tcgcctccac ctgggggtct ggggaccggc agacgccctg ccaatcctgc tcctgcatgg
     4741 aggcgccgcc aacacccact ggtgggactg ggtcgcaccg gaactggcgg ggacgtttcg
     4801 ggtcttcgcc ctggattttc ctggccacgg gcggagcgcc aggccccggc ccgtacgcta
     4861 ccgaatggcc gacttcgtgg cagccgccgc aggcgcagtt gacgccgtgg gagtgcgagg
     4921 gttgcatgtc gtggggcact ccatgggggg caaggtcgcg atgctcatgg cggctgccca
     4981 tccccgtcga gtggaacgac tggtgatcgt ggatgcgacg cccggcatct ccgcgcaggg
     5041 tttggccgac atgcggcgga tcggctcacg gcctcaacgc cttttcccgt cccgccgcct
     5101 cgccgcgcgt gcctttcgcc tgatcccgcc cgagaccgcc gccccgaggg cacgcctccg
     5161 ggccctagcc ttgtgctcga cgcgccactg cgggcacggg cgttggagca tcgggccgga
     5221 ccgcgagttc ttcagcagaa tcgtccctca ggtcgcctgg cccgtcctca cccggatccg
     5281 ctgccccacc ctcatcctgc gagcggagcg gagcagcatt ctgtcccgca ggacggcgga
     5341 ggcgatgcag agcggcatcc ctcaggcctc gctccgagag atcccagcca cctaccatca
     5401 cctgctgctg gagcagcctg cagagatcgg tcgcaccatc cgcaatttcc tgaccgggga
     5461 ggctggcctg gaatgatgcc aggctgccgt gggagtgcgg gcgcgaacgg cggggaatgg
     5521 aagatcccgg cggggctgga gatcagacga ccaagaggtc gccttcccgg gcaagctcgc
     5581 aggccgtggt cgttcccatg gccgacaccc gctgcaggac cgaggaacgg atgccgtcga
     5641 ggaagctgtc ggaatgatcc gggtcgtgat gaaacaaaat gacctgtttc accccggccg
     5701 aggcggcgag gtccaggacg tctgtccagg cggagtgccc ccagccgcgg cgctctgcga
     5761 gttcctccgg gatgtactgg gcgtcgtgga tcagcaggtc cgcgtctgca aagaagtgga
     5821 tgaaacgatc aacctggtgg gccgcatccc cgccggttgc cttccacccg aggggcaatt
     5881 cgttgtccgt ggcgtagacg atccgcttgc ctccttgcga cagtcgatag atcagggatc
     5941 ggccggggtg gaacgaggac agggtatcga actgcacgcc gcccagttgg tatgtcccct
     6001 cggcgatttc cttgaacacc atctccgctc ggaactgctc cagacccacg gggaaataca
     6061 ccgaggccat ctgtccgctc aggatctgaa gcaagttggc cgtcggttgg gccggaccca
     6121 ccagggtgaa ggaattcccg gcggtgttcg ccggctcaaa gaagggcagg ccctgaatgt
     6181 ggtcccagtg gaagtgggaa aagcacaggt agccgtgtat ggccgtcccc ggacgctgca
     6241 aggagcggcc caattcgcgg aggccggtgc cgccgtccag gatgaaggca tcgtcaatgc
     6301 cggagatccg aatctccaca catggggtgt tgcccccgta cctgaccgtc tccctggacg
     6361 ccgtggggat cgagccgcgg acgccccaga aacggaccga gatcttgcgg gccagaaggt
     6421 cgcggacgat gtttagcagc cgctccggcg tgaacggttt ttccaggaag gcgttggc
//
LOCUS       DBNGZC010000239         2564 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_117907, whole genome shotgun sequence.
ACCESSION   DBNGZC010000239 DBNGZC010000000
VERSION     DBNGZC010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_117907"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..974)
                     /locus_tag="AC16G0_04385"
     CDS             complement(63..974)
                     /locus_tag="AC16G0_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR5098894.1"
                     /translation="MTALLPALWPAAAGADAVPPAPLFPKPPPLEPNVEFWKRVYTEY
                     GIGDFLLHDRENVMIIYDVVRVSQTANERRASDLAKPEIQRLRAQYEDFLSALARGRP
                     PEELGPEGQRVAELWGCPCEQDLLLRAAANIRVQQGLREKWDEGVRRAGGLMPRIVSI
                     LRRHNVPVELAALPMVESAFNPAARSKVGAAGLWQFIRSTGRNYLTITRKRDDRHDPI
                     RSTEAAARLLKHNYEALGSWPLAIVAYNHGKAGIQTAREQLGSDDVEDIIARYNGPRF
                     GFASRNFYSEFLAALELLHPTIREHAP"
     gene            complement(1501..2034)
                     /gene="ilvN"
                     /locus_tag="AC16G0_04390"
     CDS             complement(1501..2034)
                     /gene="ilvN"
                     /locus_tag="AC16G0_04390"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR5098895.1"
                     /translation="MRHVLVALMQDEPGLLARRGNLFARRGYNVESFTAGGSEVPGLT
                     RVTIVVEGPSTTLDQVAKQFRKLIGVVKVGDVTADRDQLAHEMLLVKVGVNARTRQEV
                     VSLADLFQARIVDVSSTTLMLEATGQPDKLDKLLEVLRPFGIKEQARTGIVSLSRGAK
                     VLNQRPGSASDRKYLAD"
     gene            complement(2031..>2564)
                     /locus_tag="AC16G0_04395"
     CDS             complement(2031..>2564)
                     /locus_tag="AC16G0_04395"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR5098896.1"
                     /translation="GQHQMWVAQHYRFGRLNSFFTSGGLGTMGYSVPAAMGVQAGYPG
                     EPVWVVVGDGSFQMNMQELATIAQERLPIKILIMNNGFLGMVRQWQELFHEARYIGTP
                     ISGPDFVKLASSFGIPGRLVCAASELTDAFAEAQQADGPYLMDCRVDAAENVYPMVQP
                     GTGNADFLEDPREDAAP"
BASE COUNT          413 a          836 c          871 g          444 t
ORIGIN      
        1 cgccgccttg catctgggcc tctttgagca gcctggggaa gagcgggttt ttcggcgagc
       61 ggctagggag cgtgctcgcg gatggtgggg tggaggagct ccagggcggc gaggaactcg
      121 gaatagaagt tgcgggacgc gaacccgaac cggggaccgt tgtagcgggc gatgatgtcc
      181 tcgacatcgt cgctgccgag ctgctccctg gccgtctgga tgccggcctt cccgtggttg
      241 taggccacga tggcgagcgg ccaggagccc agcgcctcgt agttgtgctt cagcaggcgt
      301 gcggccgcct cggtggaccg gatgggatcg tgccggtcgt cccgcttcct ggtgatggtg
      361 agatagttcc tcccggtgga ccggatgaac tgccacaacc ctgccgcccc gaccttcgag
      421 cgcgccgcag ggttgaaggc ggattccacc atgggcagcg ccgcgagctc caccgggacg
      481 ttgtgccgcc gcaggatgga cacgatccgc ggcatcaatc cccctgcccg ccggacgccc
      541 tcgtcccatt tctcccgcag tccttgctgc acccgaatgt tcgcggcggc gcggagcaag
      601 aggtcctgct cacacgggca cccccagagc tcggctacgc gctggccctc cggcccgagc
      661 tcctctggcg gtctgccccg ggcgagggca ctcaggaagt cctcgtactg cgcccgcagg
      721 cgctggatct cgggcttggc caggtcggag gcccggcgct cgtttgcagt ctgcgagacc
      781 cgcaccacgt cgtagatgat catgacgttc tcgcggtcat gcaggagaaa gtcaccgata
      841 ccgtactcgg tatagacgcg cttccagaat tcgacgttgg gctccagggg cggcggcttg
      901 gggaagaggg gggcaggggg gaccgcgtcg gcgccagccg ctgccggcca gagcgccggg
      961 aggagtgccg tcaaggcgag aagagcgatg ccgagattga aggataccct tggatgaggg
     1021 cgacggatgg tcatggtcga tcagttctca cccgcgggat gaacatcggc ttcctgcgca
     1081 tcgacacctc cgtcttcccg agccatgcag gatgggggcc aaggattcgt cgtcaggaac
     1141 gggaatctgg gctcctttcc tgttggattg cggcgcgtcc agggctgcct gccccaatcc
     1201 gaagaaagac ttgaaccgcc aagacgccaa ggccgccagg atcattaagg ctcagaacat
     1261 aagtgcccgc ccgaacgcca ccgccccagg aggggtcccc gggtttcggc tctgaaacca
     1321 gcccctagag cctgtgcggc ccgaggtggc gtcttggcgg gtggtcggtt ttttcggatc
     1381 agggccagca ggcggctgct ctggggcaca tcgttttcga gtgaaaatct tgcgctggac
     1441 cgcaggactt gcgttcgaga tgtcctcgca ttctcgggcc atgggtcgga ggaaggtggc
     1501 tcagtcggcg agatacttgc ggtccgaggc ggagccgggg cgctgattga gaaccttggc
     1561 gccgcgactc agggagacga tcccagtgcg ggcctgttcc ttgatgccaa aggggcggag
     1621 gacctcgagg agcttgtcca gcttgtccgg ctgtcccgtc gcctccagca tcagggtggt
     1681 ggaggagacg tccacgatcc gggcctggaa caggtcggcc aggctgacga cctcctggcg
     1741 ggtccgggca ttcaccccca ccttgaccag gagcatctcg tgggccagtt ggtcccggtc
     1801 cgcggtgaca tcccccacct tgaccacgcc gatcagcttt cggaactgct tggccacctg
     1861 atccagggtc gtgctcggcc cctccacgac gatggtcacc cgggtgaggc ccggcacctc
     1921 cgagccgccg gcggtgaagc tctccacgtt gtagcctcgc cgggcgaaga ggttgcccct
     1981 ccgggccagc aggccgggct catcctgcat cagggccacc aagacgtgcc tcatggggcc
     2041 gcatcctccc gcggatcctc cagaaaatcg gcgttgcccg tgcccggctg gaccatcggg
     2101 tacacgttct cggccgcgtc cacccggcag tccatcaggt agggtccgtc cgcctgctgc
     2161 gcctcggcaa aggcgtccgt cagctcggag gccgcgcaaa ccaggcggcc gggaatgccg
     2221 aagctcgacg ccagcttgac gaaatcgggc ccgctgatcg gtgtcccgat gtagcgggcc
     2281 tcgtggaaca gctcttgcca ctggcggacc atgcccagga acccattgtt catgatgagg
     2341 atcttgatgg gcagtcgctc ctgcgcgatg gtggccagct cctgcatgtt catctggaag
     2401 ctgccgtcgc cgaccacgac ccacaccggc tcccccgggt agcccgcctg cacccccatg
     2461 gccgccggca ccgagtagcc catggtgccc agcccgcctg aggtgaagaa gctgttgagg
     2521 cggccgaagc ggtagtgctg ggccacccac atctgatgct ggcc
//
LOCUS       DBNGZC010000240         4396 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_20488, whole genome shotgun sequence.
ACCESSION   DBNGZC010000240 DBNGZC010000000
VERSION     DBNGZC010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_20488"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="AC16G0_04400"
     CDS             <1..586
                     /locus_tag="AC16G0_04400"
                     /EC_number="2.7.7.-"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR5098897.1"
                     /translation="MMVEAVTQHRGIINDFFGDALMAVYGAPVDDPDHALDAVRTGVQ
                     MQAGLQVLNANWKDRGLQTLQMGISVHTGSVFAGNVGSEQRMKYTVVGDAVNVAARVE
                     GLNKDLKTTLLITEETYKSIQDRVQVRDCGELKVKGRQQAIRVYEVLALSEDAAAPQR
                     RQPWWRDGGSSWGSWLFRRRRSSSPRPPETPSPS"
     gene            490..1437
                     /locus_tag="AC16G0_04405"
     CDS             490..1437
                     /locus_tag="AC16G0_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5098898.1"
                     /translation="MVARWWVILGVLALPAPAVVLAAAAGDPIAVLTEIRPGQGEARV
                     KLAIEADWKVPLPLLSLRPGDQIRVTHNATAVLMFTGGQGTVTVSAANSPYTVQVPPA
                     TAASGKSSELVANLSRLLMGKRKDLTYVPLTTRSAKNPPLLLSPRDGKLLGPPVLEWG
                     GSDRLRYTVRVSGPEGPIWEQTNLPRAPLAYPVSAPRLSPGVTYRWDLEAKDFPAQQG
                     KFMILSPTQVAAVRDTLAALSPASLSGYPKNTVTLIRAEYLIEQELYLEARMELESAI
                     TGDPDEPGLHLMLGHVHERTGLMELAAEEFDEAQFLSTR"
     gene            complement(1521..2666)
                     /locus_tag="AC16G0_04410"
     CDS             complement(1521..2666)
                     /locus_tag="AC16G0_04410"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098899.1"
                     /translation="MLAVIGHLAKKEFRQILRDRRSLVLILVMPILLTFLFGKALETG
                     ELKHIPSVILNQDESPESDVIVTAFSTYDEVKIQAEVTSLQEAQDLLARGKIKAAIVI
                     PKGFMQAVEKGEEAQLNLLLDGTDNTSAPIVEGVAQRVIQHYNSERAIKGLSAQGVRP
                     DRGRLLIQPVFVQTEIKYNPGLQYLGYVMPGVIGLTLQLLTVMLMAISIPRERERGTL
                     DQLMATPIRRVELILGKLLPYFGISLLNVITILYVADRWFNVPVGEQLPLLLLLCALF
                     VLSSLATGLLISAVSRSQFQAVQIAVFYVLPVFLLSGAYAPLEAIPDYIRPISYLFPL
                     MYFSRAVRAVTLRGADFLLVWEDVALLGGFVVIFLYWAILSFHKRVA"
     gene            complement(2670..3611)
                     /locus_tag="AC16G0_04415"
     CDS             complement(2670..3611)
                     /locus_tag="AC16G0_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5098900.1"
                     /translation="MQCAIETRDLRRHFGAVTAVAGLNLSVRTGEIFGFLGPNGAGKT
                     TTIRMLCGLLLPSGGTAAVAGLDVAKDAEAIKRCVGYMPQRFSLYGDLTVQENLDFTA
                     GLYRLTRRERKRQVGETLQRVGLAERRKTLTDHLSGGLKQRLALGSALVHSPAILFLD
                     EPTAGADPPSRQRIWDILYEVGAEGKTILVTTHYVEEAERCQTIGFMHHGRLIGTGSP
                     EQCRMDLGEVILEVDAQPVMQAATVARTQEGVTGVSIYGHTLRVFAKEAAPLSSHLQA
                     ALVASGVVVKDLRPIAPTLEDVFMSLTRGAPEDNWST"
     gene            3903..>4396
                     /gene="ispH"
                     /locus_tag="AC16G0_04420"
                     /gene_synonym="lytB"
     CDS             3903..>4396
                     /gene="ispH"
                     /locus_tag="AC16G0_04420"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743612.1"
                     /note="catalyzes the conversion of
                     1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into
                     isopentenyl diphosphate (IPP) and dimethylallyl
                     diphosphate (DMAPP); functions in the nonmevalonate
                     isoprenoid biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR5098901.1"
                     /translation="MSVKKLLLAKPRGFCAGVDRAIDVVNLALEQYPGPVYVRKEIVH
                     NVHVVNELRKKGVIFVDELDEVPDGATVIFSAHGVSPEVRACAVARGLKVIDATCPLV
                     TKVHHEAVRFAQEGRPIVLVGHKGHDEVAGTMGQAPEHIHLIGSVEAAETLQIPNPER
                     VAVIT"
BASE COUNT          803 a         1433 c         1439 g          721 t
ORIGIN      
        1 catgatggtg gaggcggtca cgcagcaccg agggatcatc aacgatttct tcggggatgc
       61 gctcatggcg gtgtacgggg ctcccgtgga cgatccggac cacgctctgg acgcggtccg
      121 gacgggagtc cagatgcagg cgggtctcca ggtgctgaac gccaactgga aggaccgggg
      181 actccagaca ctgcaaatgg gcatcagtgt tcacaccggc tccgtcttcg cggggaacgt
      241 gggctccgag cagcgaatga agtacaccgt tgtcggcgat gccgtaaacg tggcggcccg
      301 ggtggaggga ttgaacaagg acctcaagac gacgctgctc atcaccgagg agacctacaa
      361 atccatacag gacagggtgc aggttcggga ctgcggggaa ctgaaggtga aaggccgcca
      421 gcaggcgatc agggtgtacg aggtgctggc cctctcagag gatgcggcag ccccgcaaag
      481 gaggcagcca tggtggcgcg atggtgggtc atcctggggg tcctggctct tccggcgccg
      541 gcggtcgtcc tcgccgcggc cgccggagac cccatcgccg tcctgacaga gatacggcct
      601 ggccaaggcg aggcacgggt caagctggcg atcgaggcgg actggaaggt cccgctgccg
      661 ttgctctccc tccggccagg ggaccagatc cgggtaacac acaatgccac ggccgtcctc
      721 atgttcacgg gcggccaggg aaccgtcacg gtctcggcgg caaactcgcc gtacacggtg
      781 caggtcccgc cggcaaccgc ggcgagcggc aagtcatccg agcttgtcgc gaacttgagc
      841 cgcctcctga tgggaaaacg gaaggatctg acctacgtac ccctgacaac ccgaagtgcc
      901 aagaatcctc ccctcctcct ctctccgcgg gacgggaagc tcctggggcc gcctgtcctg
      961 gaatggggcg gctcggaccg gctgcgttac acggtccggg tctcgggtcc ggaggggccg
     1021 atctgggagc agaccaatct tccccgtgcc cctctggctt accccgtcag cgcccctcgt
     1081 ctgagcccgg gtgtgacgta ccgatgggat ctggaggcca aggacttccc ggcccagcaa
     1141 ggaaagttca tgatcctcag cccgacccaa gtagccgccg tccgggacac gctggccgcc
     1201 ctctcgccgg cgagcctctc gggttacccg aagaacaccg tgaccctcat ccgcgcagag
     1261 tatcttatcg agcaggagct gtacctcgag gctcgcatgg agctggaatc ggccatcacc
     1321 ggcgacccgg acgagcctgg cctgcacctc atgctgggcc acgtgcacga acggacgggc
     1381 ctaatggaac tggccgccga ggagttcgac gaggcacagt tcctttccac cagatgaaga
     1441 accgacaggg acagcgatag agacagggga actcagcgcc agaggttctg tccctgtcgc
     1501 agtccccgtc ggcttttccg tcaggcgacg cgcttgtgga agctgaggat agcccagtag
     1561 aggaaaatca ccacgaatcc gcccaggagt gccacatcct cccacaccag caagaagtcg
     1621 gccccccgca aggtcaccgc ccggaccgca cgcgagaaat acatcagcgg aaacagatag
     1681 gagatggggc ggatgtagtc ggggatggcc tccagggggg cgtacgcccc cgagagcagg
     1741 aagaccggca ggacgtaaaa tacggcgatc tgcactgcct ggaactggga ccgcgacacc
     1801 gcggagatga gcagacccgt cgccagggac gagaggacga agagggcgca gagcagcagg
     1861 agcagcggca gttgctcacc cacggggacg ttgaaccagc ggtccgccac gtacaggatc
     1921 gtgatcacgt tgagcaggga gatcccgaag tagggcaaca atttcccgag gatcagctct
     1981 acccggcgga tgggtgtggc catgagctgg tccagagtcc cccgctcgcg ttcccgagga
     2041 atgctgattg ccatgagcat cacggtgagc agttgcagcg tgagcccgat gaccccgggc
     2101 atgacgtaac ccaggtactg gagtccggga ttgtatttga tctccgtctg gacgaacacc
     2161 ggctggatga gcaggcgacc gcggtccggg cgcaccccct gggccgataa tcccttgatc
     2221 gcccgttcgg agttgtaatg ctggatgacc cgctgcgcca cgccctcgac gatgggcgcc
     2281 gaggtgttgt ccgtcccatc caggaggagg ttgagctgcg cctcctcccc cttctcgacg
     2341 gcctgcatga agcctttcgg gatgacgatg gcggccttga tcttcccccg cgccaacagg
     2401 tcctgggcct cctggaggct ggtgacctcg gcctggatct tgacctcgtc gtaggtgctg
     2461 aaggcggtga cgatgacatc gctctcgggc gactcgtcct ggttcaggat gaccgacggg
     2521 atgtgcttca gctctcccgt ctccagggcc ttgccgaaga gaaaggtgag caggatcggc
     2581 atcaccagga tgaggaccag gctccgccga tcccggagga tctggcggaa ctccttcttg
     2641 gccaagtggc caatgaccgc aagcatgagt tacgtgctcc agttgtcctc cggggccccc
     2701 cgcgtgaggg acatgaagac gtcctccagg gtgggagcga tcgggcggag atctttcacg
     2761 accacccctg aggcaacgag ggccgcttgg aggtggctgc tgagcggtgc ggcctccttg
     2821 gcgaagaccc ggagcgtgtg gccatagatg ctcacgcccg tcaccccctc ctgcgtccgc
     2881 gccaccgtgg ccgcttgcat caccggctgg gcgtccacct ccaggatcac ctcgcccagg
     2941 tccatcctgc actgctcggg cgagccggtg ccgatcagcc ggccgtggtg catgaagccg
     3001 atggtctggc agcgttccgc ctcctccacg tagtgcgtgg tgaccaggat cgtcttgccc
     3061 tcggccccca cctcgtaaag gatatcccag atccgctgcc gcgagggtgg atccgcgccg
     3121 gccgtcggct cgtccaggaa caggatcgcc ggggagtgga ccagcgcaga gcccagggcc
     3181 agacgctgct tgaggccacc cgacaggtgg tcggtgaggg tcttccgacg ctccgccagg
     3241 ccgacgcgct ggagcgtctc ccccacctgc cgcttccgct cgcggcgggt cagccggtac
     3301 agaccggcgg tgaaatccag gttctcctgc accgtgaggt cgccgtagag gctgaagcgc
     3361 tggggcatgt accccacgca gcgcttgata gcctccgcat ccttggccac atccaggcct
     3421 gccacggctg ccgtcccccc actcggcagg aggagcccgc agagcatgcg gatggtcgtg
     3481 gtctttccgg cgccgttggg tccgaggaag ccgaagatct cccccgttcg cacgctgagg
     3541 ttcaagcctg cgacggcggt caccgccccg aagtgccgcc tcagatcccg cgtctcaatc
     3601 gcacactgca tgcgccaccg atcctcctgc cccgggcctg cgaactccgc cggacccctg
     3661 cggcttctcc cccgcccaga ctcgggctct tcgggccgca ttccggcgat gtcgccttta
     3721 tactgggcga tggcgaggcc tgtcaatctt taccggcagc ttgcgtcaag ttcaagacgg
     3781 cccctttcac ccccggggcc tcgaataccg gggacaaaat gggttgacaa gccccggcaa
     3841 tctggctact gtcccggcct gaggtcatgt cgaatagagg tgcgcacctc gagaggaggg
     3901 tcatgtcagt caagaagctg ctcctggcga agccgcgagg cttctgcgcg ggagtggatc
     3961 gcgccattga tgtcgtcaac ctggccctgg aacaatatcc gggtcccgta tacgtccgta
     4021 aggagatcgt ccacaacgtc cacgtggtga acgagcttcg gaagaagggg gtcattttcg
     4081 tggacgagct ggacgaggtg ccggatgggg ccaccgtcat cttcagcgcc cacggcgtct
     4141 cacccgaggt ccgggcgtgc gcggtcgccc gcgggttgaa ggtgatcgac gccacctgcc
     4201 ctctggtgac caaggtccat cacgaggccg tacgctttgc gcaggagggg cggcccatcg
     4261 tcttggtcgg gcacaagggc cacgacgagg tcgccggcac catgggacag gcccccgagc
     4321 acatccacct gatcggctcg gtggaggcgg ccgagaccct ccagatcccc aaccccgagc
     4381 gcgtcgccgt catcac
//
LOCUS       DBNGZC010000241         2433 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_117922, whole genome shotgun sequence.
ACCESSION   DBNGZC010000241 DBNGZC010000000
VERSION     DBNGZC010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_117922"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..343
                     /locus_tag="AC16G0_04425"
     CDS             17..343
                     /locus_tag="AC16G0_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5098902.1"
                     /translation="MLTRAWRPSGPWAASGSALAGAGGVLIGVYDNQVWPTMGSVPAY
                     KCLAIVVLGGLGNIPGSVLASFLIGLAETLLIGVVAIPFPRDALAFVAMILVLMFRPH
                     GLLGKS"
     gene            456..1349
                     /locus_tag="AC16G0_04430"
     CDS             456..1349
                     /locus_tag="AC16G0_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5098903.1"
                     /translation="MLSGYVVTVLITIGIYVILALSLNMITGYAGQISLGHAAFMGIG
                     AYTSALLYTKAGLSFWPAFLLAGLVTGLVGVVLAIPCLRVREDFLAITTMGINFVVEA
                     VFLYVPFFGAGMGIGGINPPILLGKEISKTGFLVLVIAVILVVITLDRLLSRSWIGLT
                     WAAIREDEEAAGAMGIDVVGSKVLAFALGSAEAGLAGSLYAHFLTFIMPVNFNFGQSI
                     VILSMVVFGGIGTLRGPIVGAIVLGALPEVSRPAMEYRTLLYGILLLLLMRFQPDGIL
                     GEQSLLVRGWRSLRQRLVPVR"
     gene            1506..2255
                     /locus_tag="AC16G0_04435"
     CDS             1506..2255
                     /locus_tag="AC16G0_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5098904.1"
                     /translation="MALLEIEHLTKEFDGVTALQDVTFDVREGEILGVIGPNGAGKTT
                     LFNCITGMMPPSRGAIRYRGEAIQGKKAFEIARKGIARTFQIVRPFPTLSLTDNVLVA
                     YGHRHYPRLRAAIAAFSTPANRDAATALLLRVGLSAARDRPAGTLPLGLKKRLEIARA
                     LALDPSLLLLDEPSGGLRHEESQQLLELIRRLNQEAVSIILIEHNMPIVMGVCQRLVV
                     LDHGAKIAEGDPATIRKNPQVIEAYLGKAGV"
BASE COUNT          395 a          768 c          765 g          505 t
ORIGIN      
        1 ggcatctacg ccctgggtgc ttacgcgggc ctggcggcct tccgggccgt gggcagcttc
       61 tggctcagcc ctggcgggcg cgggcggcgt cctcatcggc gtctatgaca accaggtatg
      121 gccgaccatg ggcagcgttc cagcctacaa gtgcttggcg attgtcgtcc tgggcggcct
      181 ggggaacatt ccgggaagcg tcctggcctc gttcctgatc gggctggccg agaccctgct
      241 catcggggtt gtggccattc cttttccgcg ggacgccctc gcatttgtgg cgatgatcct
      301 ggtgctcatg tttcgccctc acgggcttct ggggaagtcc tgaagatgct gaatggccaa
      361 ttcccaaatc ccacctcgtc attcgtcatt ggccacaggt cattggtcat tgaatctcgg
      421 ctcgcggctc gcggctcgcg gctctcgact cgaccatgct aagcggttat gtggtcaccg
      481 tcctcatcac cataggtatc tacgtgatcc tggccctgag cctgaacatg atcacgggct
      541 acgcaggcca gatctcgctc ggccacgccg cgttcatggg gatcggcgcc tatacctcgg
      601 ccctcctcta caccaaggcc gggctcagct tctggcccgc cttccttctg gcgggtctcg
      661 tgaccggctt ggtcggcgtg gtcctggcca tcccgtgtct tcgcgtccgt gaggacttcc
      721 tggccatcac caccatgggc attaacttcg tggtggaggc cgtcttcctg tacgttccat
      781 tcttcggcgc tgggatgggg atcggaggga tcaatccacc gatcctcctg ggcaaggaga
      841 tcagcaagac aggcttcctc gtcctggtga ttgccgtgat cctggtcgtg atcacgctgg
      901 atcggcttct ctcccgttca tggatcggcc tcacctgggc tgccatccgg gaggatgaag
      961 aggctgccgg cgccatgggc attgatgtgg tggggtccaa ggtcctggcc tttgccctgg
     1021 ggagcgccga ggccgggctg gccggcagcc tgtacgctca tttcctgacg ttcatcatgc
     1081 cggtcaactt caacttcggc caatccatcg tcatcctctc catggttgtc ttcgggggga
     1141 tcggaaccct ccgcggcccc atcgtagggg ccatcgtcct gggggcattg ccggaggtct
     1201 cccggccggc catggagtac cgaacgctcc tgtacggcat cctcctcctc ttgctcatgc
     1261 gctttcagcc ggatggcatc ctgggggagc agagcctgct cgtccgcggc tggcgctcgc
     1321 tgcgccagcg actggtacct gtacggtaag tggttcgtgc cagtgcagaa tcgataagat
     1381 ggaggatgga cacgtgccaa tgcaaaaccg acaacggaca acggacaacc ggtcagccgg
     1441 ggccttcgtt ggtcggttgt cggttgtcag ttgttcgttt tagattggca ctttccagga
     1501 tcacgatggc tctccttgaa atcgaacatc ttaccaagga attcgacggc gtcacggctc
     1561 tgcaggatgt gacgttcgac gtgcgcgagg gagagatcct cggcgtcatc ggacccaacg
     1621 gggccggcaa gacgacgctc ttcaattgca tcacggggat gatgccgccg agtcgggggg
     1681 cgatccggta tcgaggtgaa gccatccagg ggaaaaaggc attcgagatt gcccgcaagg
     1741 gcatcgcccg cacctttcag atcgttcggc ccttccccac cctctccctc accgacaacg
     1801 tcctggtggc ctacgggcat cgccattacc ctcgcctccg cgccgccatc gcagccttca
     1861 gcaccccggc caacagggat gccgccacgg ctttgctcct gcgggtgggg ctctcggcag
     1921 cccgcgaccg gccggccggg accctcccgc tggggctgaa gaagcggctg gagatcgcac
     1981 gcgccctggc gctcgatccc tcgctgctct tactggacga gccgtcgggg ggacttcgtc
     2041 atgaggagtc ccagcaactc ctggagttga tccggcgact gaatcaggag gccgtcagca
     2101 tcatcttgat cgagcacaac atgcccatcg tgatgggcgt atgccagcga ttggtggtcc
     2161 tggatcacgg cgccaagatc gccgagggcg atcccgcaac catccggaag aacccgcagg
     2221 tgatcgaggc ctaccttggg aaagccggcg tgtagggggg tgcccagggc gtatggcact
     2281 ggggatgcgg ctcagagggc tgttgcccgt ccggccgcca tatcgcatac gccagaggca
     2341 ctcctgcatg agagccatgc ttgaagtccg gaatctcgaa gtgacatacg gcgagttcgc
     2401 cgcggtccga ggcgcgagct tcgaagtcaa gag
//
LOCUS       DBNGZC010000242         2007 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2465860, whole genome shotgun sequence.
ACCESSION   DBNGZC010000242 DBNGZC010000000
VERSION     DBNGZC010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2465860"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..>2007
                     /locus_tag="AC16G0_04440"
     CDS             86..>2007
                     /locus_tag="AC16G0_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5098905.1"
                     /translation="MISEFFIERPIFANVIAFITIIIGAVFLYRLPVAQYPEIVPPTI
                     QVSTRYPGASAEVVAATIGVPLEQAINGVENSIYMSSTSSSDGSYTLTITFDVGTDLD
                     TSLALVQNLANSALSQLPGGVTAQGITVRKVSPNILLVASLYSEDDRYDETFFSNYAI
                     INLQNPLARIQGVGQIRIFGAGPYSMRVWLDPKRLQTFGLTTQDVLNAIRGQNIEVVA
                     GQIGGPPVPEDQPFQFTINALGRLSDAREFDAITIKSARGTAPQLVRLRDVARVELSQ
                     QSFSNFSRFTGHKAAQIVVFALPGANAIQVADRVYAAMAEMSRQFPEGMKYAIRYDTT
                     LFVREAISAVYQTLFLAGILVLIVILVFLQNFRGMLVPATTVPVTIIGAFIVMAALGF
                     TVNLMTLFALILAIGIVVDDAIIIVENSSYYIEQGMPPKDATIKAMQELTGPVMGITL
                     ALVSVFLPAAFLPGITGQIFRQFALVIASTAVISAINALTLKPVQCALWLKPRWEKAP
                     NWFYRGFNRVFGAMTGVYVGTVRWMVKHPVLMLMVFVVIVSAAAWGFVRQPTGFLPTE
                     DQGYAILVSILPEGASQPRSRDVAEKINAILQKTDGLAGWVTIGGFSVLDFANIANVS
                     TTFLVYKNWKERGEGLT"
BASE COUNT          359 a          696 c          580 g          372 t
ORIGIN      
        1 ggcaggtcac tcctgaacag gaaggaggcg cacgcctgcc ggagcaaggc gattcgccgt
       61 cgcttccgcc gcggaaggcc aggccgtgat ctccgagttc ttcatcgaac ggccgatctt
      121 cgccaacgtc atcgccttca tcaccatcat catcggggcc gtcttcctct atcgtttgcc
      181 cgtcgcccag tatcccgaga tcgtgccgcc gaccatccag gtgagcacgc gatacccggg
      241 cgcgagcgcg gaggtggtcg cggccacgat cggggtgccg ctggagcagg cgatcaacgg
      301 cgtcgagaac tccatctaca tgtcctcgac cagctccagc gacggctcgt acaccctgac
      361 catcaccttc gacgtcggca cggacctcga cacctccctg gccctggtgc agaacctcgc
      421 caacagcgcc ctgtcccagc tcccgggagg cgtcaccgcc caggggatca cggtccggaa
      481 ggtctccccc aacatcctgc tggtggcgag cctctattcc gaggacgatc gctacgacga
      541 gaccttcttc tccaactacg cgatcatcaa cttgcagaac cccctggccc ggatccaggg
      601 cgtcggccag atccggatat tcggagccgg cccctacagc atgcgggtct ggctggaccc
      661 gaagcgcctg cagaccttcg ggctcacgac ccaggacgtg ctgaacgcca tccggggaca
      721 gaacatcgag gtggtcgccg ggcagatcgg cggcccgccg gtccccgagg atcagccctt
      781 ccagttcacg atcaacgccc tcggccggct ctccgacgcc cgcgagttcg acgcgatcac
      841 gatcaagtcg gcccgcggca ctgcccccca gctcgtgcgc ctccgtgacg tcgcccgggt
      901 cgaattgagc cagcagagct tcagcaactt ctcgcgattc accggtcaca aggcggcgca
      961 gatcgtggtg tttgccctgc cgggcgccaa cgccatccag gtcgccgatc gcgtgtatgc
     1021 ggccatggcc gagatgagca ggcagtttcc cgagggcatg aaatacgcga tccggtacga
     1081 taccaccctc ttcgtccggg aggccatctc cgcggtttac cagaccctgt tcctcgccgg
     1141 aattctcgtc ctcatcgtga ttctcgtgtt cctgcagaac ttccggggaa tgctcgtacc
     1201 cgcgacaacc gttccggtca ccatcatcgg cgccttcatc gtcatggccg cgctcggttt
     1261 caccgtgaac ctcatgaccc tgttcgcgct gatcctggcc atcggtatcg tggtggacga
     1321 cgccatcatc atcgtggaga acagctccta ctacatcgag cagggaatgc cccccaagga
     1381 cgccaccatc aaggccatgc aggaactcac ggggccggtg atgggcatca ccctggctct
     1441 ggtgtccgtc ttcctgccgg ccgccttcct gccgggaatc accgggcaga tcttccgcca
     1501 gttcgcgctg gtcatcgcct cgaccgccgt catcagcgct atcaacgcgc tgaccttgaa
     1561 gccggtccag tgcgcgctct ggttgaaacc gcgctgggaa aaggctccca actggttcta
     1621 ccgtggattc aaccgcgtct tcggggcgat gaccggcgtc tacgtgggaa ccgtcagatg
     1681 gatggtgaaa cacccggtgc tcatgctcat ggtcttcgtc gtcatcgtca gtgcggccgc
     1741 ctggggattc gtgcgccagc ccacaggctt tctccccacc gaggaccagg ggtatgccat
     1801 cctcgtgtcc atcttgcccg aaggcgcatc ccagccacga tcgcgtgacg tagcagaaaa
     1861 gatcaacgcc attctccaga aaacggacgg gctcgcgggg tgggtgacca tcgggggctt
     1921 ctcggtcctc gattttgcca acatcgccaa tgtgtcgacg accttcctcg tgtacaagaa
     1981 ctggaaggaa cggggcgaag gtttgac
//
LOCUS       DBNGZC010000243         1690 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1879359, whole genome shotgun sequence.
ACCESSION   DBNGZC010000243 DBNGZC010000000
VERSION     DBNGZC010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1879359"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..654
                     /locus_tag="AC16G0_04445"
     CDS             127..654
                     /locus_tag="AC16G0_04445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5098906.1"
                     /translation="MSIGAFDTVVSAHHAEIYRYLQRAIGRTGDADDISQETFLRAFR
                     AYGSLPRDANVRAWLFSIATNLAKNYFRAESRRRKAYEEVRASARETVGSVAEAEVVS
                     RETGELVEGIVQRLPLKQRLAFTQRKIHGLDYEAIGRSLGCSAESARAHVFQAVRKIR
                     QALDGHGLLPEEPQA"
     gene            651..1619
                     /locus_tag="AC16G0_04450"
     CDS             651..1619
                     /locus_tag="AC16G0_04450"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR5098907.1"
                     /translation="MSDKPTVCVQIEDDLLATALGEAAPGAARRVERHLAACGSCRRE
                     FQEYRAVDGAVSVLRDEPPPSGRLAQVREQLETQLADLRSRLLAYRIFPSPLGHILIA
                     RSELGISLVEYLERGTSLKASHLYHEPGVEFVEAGGELETFHRELSEYLEGRRKRLEW
                     PLDLRLARSDFHRTVLRATAAIPYGAVIPYAGIAQEVGKASAVRAVAQALRWNPLPIV
                     IPCHRVIGTSGALTGYAGNKVGLKQRLLSLEGVPTARKAGVLGISPQTMYILYPGEQE
                     YCLPTCPSFETHRPAPLTRFASREYAEAAGFAPCTTCRPDLHPISH"
BASE COUNT          294 a          544 c          563 g          289 t
ORIGIN      
        1 ttgtgccagt ttggcacaag ggggtctgcc taccggattc ggccaagact tctcggatct
       61 aagcccccag gagggcttgc gcgggcttca tttcacgctg gagcagcagc ggtgtctaat
      121 aatgtgatga gtatcggagc gttcgacacc gtcgtatcgg cgcatcacgc agagatttac
      181 agatatctcc agcgggcgat cggtcggacc ggcgacgcgg acgacatctc acaggagacg
      241 tttctccgcg cgtttcgagc gtacgggtct ctgcccaggg acgccaacgt ccgggcttgg
      301 ttgttttcca ttgctaccaa cttggccaag aactacttcc gtgcagagag tcgtcgcagg
      361 aaggcttatg aagaggttcg cgcctccgcg cgtgagactg tcggttccgt ggcggaggcc
      421 gaggtggttt ctcgggagac tggagagctt gtggagggga tcgtccagcg tctcccgctc
      481 aagcagcgcc tggctttcac acaacgcaag attcacggcc tcgattacga ggcgataggc
      541 cggagcctgg gctgctcggc cgagagcgcc cgggcgcatg tgttccaggc ggttcgaaag
      601 atccgtcaag ccctcgatgg ccacgggctg ctccccgagg agccgcaagc atgagcgaca
      661 aaccgacagt atgcgtccag atcgaagatg acctcctggc cacggcgttg ggcgaggcgg
      721 cgccaggcgc ggcgcggcgc gtggagcgcc acctggccgc gtgtggctcc tgccgccggg
      781 agttccaaga gtatcgcgcg gtggacgggg ccgtgagcgt cctgagggac gaaccgcccc
      841 cgagcgggcg cctggcccag gtccgggagc aactggaaac gcagctggcg gatctgcgaa
      901 gccgcctgct ggcctatcgc atcttcccgt cgccccttgg gcatatccta atcgccaggt
      961 ccgagttggg gatctcgctg gtcgagtacc tggagcgggg gacgagccta aaggcgtcgc
     1021 atctctacca cgaaccgggc gtggagttcg tcgaggccgg cggggaactc gagaccttcc
     1081 accgggagct gagcgagtac ctcgagggcc ggcgcaagcg actcgagtgg ccgcttgatc
     1141 tgcgcctggc gcggagcgac ttccaccgga cggtgctccg ggcaacggcg gcgatcccat
     1201 acggagccgt gatcccctac gccggcatcg cccaagaggt cggcaaggct tcggccgtgc
     1261 gcgccgtggc ccaggcgctc cgctggaacc cgctgccgat cgtgatcccg tgtcaccgcg
     1321 tgatcgggac ctcgggcgcc ctgaccgggt acgccggtaa caaggttggc ttgaagcaaa
     1381 gactcctctc cctcgagggg gtgccgaccg ccaggaaagc tggcgtcctg ggtatctctc
     1441 ctcagacgat gtacatcctc tatcccgggg aacaggaata ctgcctgccg acgtgcccct
     1501 ccttcgagac acaccgcccg gcgcccctca ctcgcttcgc ctcccgggag tatgccgaag
     1561 cggcgggctt cgccccctgc accacctgcc ggccagacct ccacccgatt tcacactagc
     1621 cggcctccgg tgtcctaggg aatgaaactg gcacccagga cagctcacac ggggaggagg
     1681 gcgaacatgg
//
LOCUS       DBNGZC010000244         5131 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1682399, whole genome shotgun sequence.
ACCESSION   DBNGZC010000244 DBNGZC010000000
VERSION     DBNGZC010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1682399"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..565)
                     /locus_tag="AC16G0_04455"
     CDS             complement(65..565)
                     /locus_tag="AC16G0_04455"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR5098908.1"
                     /translation="MKPPSIPLEKILALQQACLELTVPESYRDQNGHMNMRWYVAIFD
                     DAGDSLYLQLGLTPEYHARHGTGTFDLEHHTHFLSEVVPGDRVAVYVRLVARSTKRFH
                     YLMFMVNQTRGKLAAIFECMTAFADLKVRRTAPLPPEIALEVEAGVAAGTALDWPAPV
                     CGAMHV"
     gene            complement(597..1640)
                     /gene="rlmN"
                     /locus_tag="AC16G0_04460"
     CDS             complement(597..1640)
                     /gene="rlmN"
                     /locus_tag="AC16G0_04460"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR5098909.1"
                     /translation="MSQKLVDLVGLGRAEVEELMAELGQPRFRGRQIFHWVQAHRCVD
                     VVAMTDLSQALRAELGQRMQVTRPEIVRAQRATDGTRKYLLRLADGEEIESVLIPDED
                     RLTACISSQAGCPLACRFCLTGLMGLRRNLTAAEITGQVLLLQDALEGGERISNVVLM
                     GMGEPLLNFPQVERALRILTDEHGANFSPRRITVSTAGHVPGIQKLAASDLGVNLAVS
                     FSATTDEVRGQIMPINRRWPIAELLAACRAYPLPNRRRLTFEYVLLDRVNDSLEDAGR
                     LANLLREIRCKVNLIPLNAAPDLPDRPSPRPRVEAFQRVLHDAGMTATIRESRGWDIS
                     AACGMLRVEKEDA"
     gene            complement(1637..2155)
                     /locus_tag="AC16G0_04465"
     CDS             complement(1637..2155)
                     /locus_tag="AC16G0_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098910.1"
                     /translation="MTFVERDGQTARPAVDLKGHTLHLPIWAFRVQYILQWEDVQRES
                     LARQVPLVEWVYVTGFELHNASYFGDPGLIFTERRVRLDPGPPAPLAGCTRGLEEAKA
                     YVEPHLLTIIDRRADVTGLELRCSISAARLWGVPYADGGAVLQDGILGLKIPAAAVDE
                     VGAMRQLLSEAG"
     gene            complement(2465..3586)
                     /locus_tag="AC16G0_04470"
     CDS             complement(2465..3586)
                     /locus_tag="AC16G0_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HIR5098911.1"
                     /translation="MSLTHLGCPSCGGTLSLAEGQRLVACQYCGGESLVSVPGAVPRY
                     AVMQGVSQEEARAAAQRLLVTPGLPRGLRERGRIQDVSLCYVPFYEFTGTRLGTFFLR
                     EKEKSSAPLTGEGVEDVEFQRWLLEPPVVREDTRVIQQDYVRIGPACEQPELGLDRIP
                     LASLRRGATPVALEPFDLVALQTRAVVLAPTKAPQGFADESQWRFKVRSDRTAFVEQR
                     LKLLYYPVWQARYLFRGRPYEVAVDGVTGKVLRGRAPAEMRPAVALAVAALALGAFCF
                     GRPGRQLVLSSLATGGQARWVFGTFGVLVALFLAGTVASLLAWMAWTIFQQSGEMILG
                     DEGSEIQLDLGSEEGGLQQIIASGMRRLLAGGWGTSKHN"
     gene            complement(3691..4431)
                     /locus_tag="AC16G0_04475"
     CDS             complement(3691..4431)
                     /locus_tag="AC16G0_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098912.1"
                     /translation="MKVPCPQCSGEIQLHDTTGLVGCPFCGTSLVLDLTGVRLHLLYR
                     PRHAGGSVLPILRRWCDSQRLPLPSLLSAPHLVYYPFWRFRAAGRSRLAAAWPTLEAR
                     WADIPPPEGEVVVYDHTTVGSAQVVEASVAEAAARARTLGKGATTPGDLIHIPFFEAP
                     VRIGGHQVLVSLEACSAHVYPDRVPRGARGLSGRPAFSAWTGILGSLAMALEAMLIPA
                     AWMAAIAVALTAVAFYWAILSDAAGTAS"
     gene            complement(4428..>5131)
                     /locus_tag="AC16G0_04480"
     CDS             complement(4428..>5131)
                     /locus_tag="AC16G0_04480"
                     /inference="COORDINATES: protein motif:HMM:NF024813.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR5098913.1"
                     /translation="EQVEGSLREGIVTRVNDFLGEPVDTILQLPRQYDEIGTAVKVRL
                     ADDFRRYGLELIDFFVNAITPPPEVQRMIDERSGMAAVGNLDDFMKFKAAKAMGDAAA
                     GVGSGGGQAAGGLGMGLGAGLGFMLPGMIYGALRPEDADPRRIQERGTVNCPACHGDV
                     SLTSRFCPACGHQMVVANKCPRCGEDVTAQAKFCPACGTSLSATLACGHCKTVVPPGT
                     KYCTNCGEAIAPQTP"
BASE COUNT          977 a         1768 c         1555 g          831 t
ORIGIN      
        1 gcgaagacgc atccggagct ggggcgcgcc gctccacctg gggaatgcag tggagtactt
       61 cgcgtcacac gtgcatcgca ccgcacacgg gtgcgggcca atccagtgcg gtacccgcgg
      121 caacgccggc ctcgacctcc agcgcaatct ccggtggcag cggcgcggtc ctgcggacct
      181 tcagatcggc gaaggcagtc atgcactcga aaattgcagc caacttccca cgcgtctggt
      241 taaccatgaa catcagatag tgaaagcgtt tggtcgagcg cgcgacgagc ctgacgtaca
      301 ccgccacgcg gtcaccgggg acgacttcac tcaagaagtg agtgtgatgc tcgagatcga
      361 acgtgccggt cccatgccgg gcgtgatact ccggcgtcag accgagttgg aggtacaggc
      421 tgtcgcccgc atcgtcgaag atcgcgacat accaccgcat gttcatgtgc ccgttctggt
      481 cccgatagga ttcggggact gtcagctcaa gacatgcctg ctggagcgcg aggatttttt
      541 ccaggggaat gctggggggt ttcatgcact cgtctcggcc accctgtatc cgcggttcag
      601 gcgtcttctt tttcaacgcg gagcatcccg cacgccgcgg agatgtccca ccctcgactc
      661 tcccgaatcg tggccgtcat gccagcgtcg tgcaggacgc gctgaaacgc ctccacgcgc
      721 ggccggggtg acggacgatc tggaaggtcc ggcgccgcgt tgagggggat gagattgacc
      781 ttgcagcgaa tctcgcgaag gagattcgcc agtcgtccgg cgtcctccag ggagtcattc
      841 acccgatcca ggaggacgta ttcgaaggtc aggcgcctcc gattgggcaa gggataggcg
      901 cgacaggctg cgagcagctc ggcaattggc cagcgcctgt tgatgggcat gatctggccg
      961 cgaacctcgt ccgtggtggc gctgaaggag accgccaggt tgacccccag atccgacgcc
     1021 gccagctttt gaatccccgg aacgtgaccc gcggtggaca ccgtgatccg ccggggggag
     1081 aaattcgccc cgtgctcgtc agtcaggatc cgcaacgccc gctccacctg cgggaagttg
     1141 aggagcggtt cccccatccc catcagcaca acattggaga ttcgctcccc accttccagc
     1201 gcatcctgca ggagcaagac ctgtcccgtg atctcggcag ccgtcaggtt gcggcgaagg
     1261 cccatgagac cggtgagaca gaaccggcaa gccagcggac agccggcctg cgaggagatg
     1321 caggcggtca ggcggtcctc gtcggggatc aggacgctct caatctcttc gccatcggcc
     1381 agccggagga gatacttccg ggtcccgtcc gtcgcgcgct gggcccgaac gatctccggg
     1441 cgggtcacct gcatgcgttg gcccaactcg gcgcggaggg cctgggacag gtccgtcatc
     1501 gccaccacgt ccacacatcg gtgggcctgc acccagtgga agatctgtct gccacgaaag
     1561 cgcggctgac cgagttccgc catcaactcc tcgacctccg cccgaccgag gccgaccagg
     1621 tccaccagct tctggctcat ccagcctccg agagcaattg ccgcatggcc ccaacttcat
     1681 ccaccgcagc cgcggggatc ttcagcccca ggatgccgtc ctggagcaca gccccgccgt
     1741 cagcataggg gacgccccag agtcgggcgg cactgatgga gcaacgtaat tccagcccgg
     1801 tcacatccgc gcggcggtcg atgatcgtga gcaggtgagg ctccacgtac gccttggcct
     1861 cttccaggcc tcgcgtgcag cctgccaaag gcgcgggagg acccggatcc agccgcaccc
     1921 gcctctcggt gaagataagc cccggatcgc cgaagtaaga ggcgttgtgc aactcgaacc
     1981 cagtcacgta cacccactcc accaatggca cctggcgagc caaagactct cgctgcacgt
     2041 cctcccattg cagaatatac tgcacccgga aggcccaaat gggaagatgc agggtatgtc
     2101 ccttcaggtc tacggcgggg cgcgccgtct ggccgtctcg ctcgacgaag gtcatcccca
     2161 cgacctcgcg aggggcacca caacctctgc accagaacgc cacgtcttgt tggaggccaa
     2221 gcaagggccc gccgcatcgg ggacaccgga gagacaggac actcggttcg gtcacgtcaa
     2281 ttgacccgca gaggaaccac ccgatgtttc accttcagat tccgactaca acgacgatca
     2341 gatatcagac tcaagagctt tggttcgcat actgatgctt gccgctattc caataactta
     2401 cggtaattcc atcagacacc tgttgatgga aaatcatccg gaaattcaac gcctggcgcg
     2461 tccttcagtt gtgtttggac gtaccccaac cgcctgccag cagccgccgc atcccggagg
     2521 cgatgatctg ttgcaggccc ccctcctctg atccgagatc caactgaatc tcgctccctt
     2581 catcacccag gatcatctcg cccgactgct ggaagatcgt ccaggccatc cacgccagaa
     2641 gcgaggcgac cgtgccggca aggaagagcg ccaccaatac tccgaacgtc ccgaacaccc
     2701 acctcgcctg accacccgtg gcgaggctgc tcaggacgag ctgccgtccc ggacgtccga
     2761 agcaaaacgc gcccagtgcc agggcagcca ccgccaaggc cacagccggt cgcatctctg
     2821 ccggtgcccg cccgcgaagc accttccccg tgaccccgtc caccgcaact tcatacggac
     2881 gacctcggaa gagatagcgt gcctgccata ccggatagta gaggagcttc aggcgttgtt
     2941 ccacgaaggc ggtgcgatcg ctccggacct tgaagcgcca ctgcgattca tcggcgaagc
     3001 cctggggggc cttggtcgga gcaagaacca cagcccgggt ctgaagggcg acaaggtcga
     3061 agggttcgag cgccacgggt gttgccccgc gccgcaggct cgccagcggt atacgatcca
     3121 atcccaattc cggttgctcg caggccgggc cgatccggac gtagtcttgc tgaatgaccc
     3181 gcgtgtcctc tcgcaccacc ggaggctcca gcagccagcg ctggaactcg acatcctcca
     3241 caccttcccc ggtcagtggg gccgaggatt tttccttctc ccggaggaag aaagtcccga
     3301 gacgggtgcc cgtgaactca taaaacggca catagcacag ggagacatcc tggatccttc
     3361 cccgttcccg cagcccgcgg gggagcccag gggtgaccaa gaggcgctgg gctgccgccc
     3421 gagcctcctc ctggctgacc ccctgcatca ccgcatatcg cggcaccgcc ccgggaaccg
     3481 aaaccaggct ctcccctccg caatactggc aagccacgag gcgctgccct tccgccaagg
     3541 agagggtccc gccgcacgac ggacagccca gatgcgtcag cgacacgctc taccccccac
     3601 tgcaaatgtc gattttcagc ttgaagaatc gcatgcagca cacccgcagt tcgattccaa
     3661 ggtggcgcac tcgaaatgcg ctatccgaaa tcacgacgcg gtgcccgctg catcgctcag
     3721 gatcgcccag taaaacgcca ctgccgtcaa cgccacggcg atggccgcca tccaggccgc
     3781 cgggatgagc atggcctcca gcgccatggc cagagagccc aagatcccgg tccaggcgct
     3841 gaaggccggt cgtcctgata ggccgcgagc gccgcgcggg actcgatcgg gatagacatg
     3901 agccgagcag gcctccaggc tgaccagcac ctgatggccg ccgattcgca ccggcgcttc
     3961 aaaaaagggg atgtggatca ggtcacccgg tgtggttgcg cctttgccca aggtccgggc
     4021 acgcgccgcg gcctccgcca cgctcgcctc caccacttgc gcgctcccca ccgtcgtatg
     4081 gtcgtagacg actacctccc cttcgggggg cgggatgtcg gcccaacgag cctcgagggt
     4141 gggccacgcc gcggcaagac gcgagcgtcc cgccgccctg aagcgccaaa aggggtagta
     4201 cactaggtgg ggagccgaaa gcagcgacgg cagggggagg cgctgggaat cacaccatcg
     4261 ccggaggatg ggcagaacgc ttcctccggc gtgccgtggc cggtacagga gatgcaaccg
     4321 gacgcccgtc aggtccagga ccagactcgt gccgcaaaag gggcacccga ccagtccggt
     4381 cgtgtcgtgg agttggattt ccccgctgca ctgcgggcac gggaccttca cggcgtctgt
     4441 ggagcgatgg cctcaccgca gttcgtgcag tacttcgtgc ccggtggcac caccgttttg
     4501 cagtgaccgc aggccagcgt cgcgctgaga ctcgtcccgc acgcggggca gaacttggcc
     4561 tgggcggtca cgtcctcgcc gcagcgggga cacttgttcg ccaccaccat ctggtgccca
     4621 caggccgggc agaatcggct cgtcagggac acgtctccat gacacgcagg gcagttgacc
     4681 gtgccccgct cttggatgcg ccgcgggtcc gcgtcctcgg gccggagcgc cccgtagatc
     4741 atgccgggca gcataaaccc caagcctgct cccagtccca tgcccagacc acccgccgcc
     4801 tgtccgcccc cgctccccac cccggctgcg gcatcgccca tggccttcgc cgccttgaac
     4861 ttcatgaagt cgtccaggtt cccgacggcg gccatgccag accgctcatc gatcatgcgc
     4921 tgcacctcgg gtggcggcgt gatggcgttg acgaagaaat ctatcagctc caggccgtag
     4981 cggcgaaaat cgtcggcgag ccggaccttg acggccgtgc cgatctcatc gtactgccgg
     5041 gggagttgga ggatggtgtc cacgggttct cccaggaagt cgttcacccg cgtgacgata
     5101 ccctcccgga gagatccctc cacctgctcc g
//
LOCUS       DBNGZC010000245         1467 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2269998, whole genome shotgun sequence.
ACCESSION   DBNGZC010000245 DBNGZC010000000
VERSION     DBNGZC010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1467)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2269998"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="AC16G0_04485"
     CDS             <1..202
                     /locus_tag="AC16G0_04485"
                     /inference="COORDINATES: protein motif:HMM:NF013927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR5098914.1"
                     /translation="SYRQIVDLGDPEASRWSLAGGTSGDPRSPHYADQIEAWLRGEYR
                     SMHLSSKAEASEGTVLRLLPAP"
     gene            273..1454
                     /locus_tag="AC16G0_04490"
     CDS             273..1454
                     /locus_tag="AC16G0_04490"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR5098915.1"
                     /translation="MRSWPIPAEPWPFVLGRELVGVFAVDEWQAQDGMVRFRGRLLVE
                     PEVATSLLSQRMAPFGYFPMIRSREEITLIRLDTGYGGKEVTTWRGWPLNLALFLATV
                     LTTLFVGALIEGASPLSDPWSLILGVPFSASLLLILGFHELGHYLVARAYGVHVSLPY
                     FIPLPLPPMGTMGAIIRMRSPIPNRKVLFDIGIAGPLLGLLLAVPVLSIGLMLSPVKA
                     VSGVVLQEGNSLAYLFLKWLIKGPIPPGSDVMLHPMALAGWLGLFVTVLNLMPLSQLD
                     GGHIVYAVLGRGHRKIVWLFLGVMVVLYAVSRWEGWLVWVALSVALGLKHPPPLDDLT
                     PLDGPRRILALIALVLLVVLMTPRPFAVYEFLPHPDPPLGSTGPGEAPPGNPGAPLPP
                     G"
BASE COUNT          190 a          516 c          457 g          304 t
ORIGIN      
        1 aagctaccgt cagatcgtgg acctggggga tccggaggct tccagatggt ccctggcagg
       61 ggggacttcg ggcgaccccc gttcgcctca ctacgccgat caaatcgagg catggctccg
      121 aggggagtac cggtccatgc atctctcatc caaggccgag gcatccgaag ggaccgtctt
      181 gcgccttctt cccgctccat gaggcgattg cgtccgcctt tctcccatgt tataaccgcc
      241 ccatgccctg gcggttgagg aggatatcct gaatgcgtag ttggcccatc ccggcggaac
      301 cgtggccgtt tgttctgggg cgagagctcg tcggtgtctt cgccgtcgac gagtggcagg
      361 cccaggacgg aatggtgcgc ttccgcggtc gcctcctggt cgagcccgag gtggccacct
      421 ccctcctttc ccagcggatg gctcccttcg ggtacttccc catgatccgg agccgggagg
      481 agatcaccct catccgcctg gacaccggct acggcggcaa ggaggtcacg acctggcggg
      541 gctggccgct gaatctggcg ctcttcctcg ccaccgtgct cacgaccctc ttcgtggggg
      601 ccctcattga gggggcgtct ccgctatccg acccatggag tctgatcctg ggtgtgccct
      661 tctctgccag cctcctcctg atcctcggct tccacgagct gggacactac ctggtggcgc
      721 gggcctatgg cgtccacgtg agcctgccct acttcatccc gctgcccctg ccccccatgg
      781 ggaccatggg ggcgatcatc cgcatgcgct cgcccattcc caaccggaag gtcctcttcg
      841 acatcgggat cgccggtccg ctgctgggtc tcctgctggc cgttcccgtg ctcagcatcg
      901 ggctcatgct ctcgccggtg aaggccgtgt ccggcgtggt gctgcaagag ggaaactccc
      961 tcgcctatct cttcctgaag tggctgatca aggggccgat cccgccgggc tccgacgtga
     1021 tgctccaccc catggccctg gcgggatggc tgggcctctt cgtcacggtc ctgaacctca
     1081 tgcccctgtc acagctcgac ggcggccaca tcgtgtatgc ggtcctgggc cgagggcacc
     1141 ggaagatcgt gtggctgttc ctgggggtca tggtcgtgct ctacgcggtg tcgcgctggg
     1201 aggggtggct ggtctgggtg gccctgtcgg tggcgctggg gctcaagcat ccaccgcccc
     1261 tcgacgacct gacgcccctc gacggccctc gccggatcct cgccctgatc gccctggtgc
     1321 tgctggtggt cctgatgacg cctcgcccgt tcgccgtcta tgagttctta ccgcaccccg
     1381 atcctcccct cgggtcgact ggccctgggg aagccccccc tggaaatcct ggagcgcctc
     1441 ttccgcctgg ttgacatcac cgatcct
//
LOCUS       DBNGZC010000246         2264 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2074266, whole genome shotgun sequence.
ACCESSION   DBNGZC010000246 DBNGZC010000000
VERSION     DBNGZC010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2074266"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="AC16G0_04495"
     CDS             <1..432
                     /locus_tag="AC16G0_04495"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR5098916.1"
                     /translation="REPSDNDARASAQRENLLIDRDGTTGRDQESLLRITLADLAVTR
                     DIRESGELAELALSSLDKILRIENRGVKSGPFYVLATVAMPAVLVESAFITNPNEERK
                     LQRDAYRQRIAEALCEGIGKFKARYERRVGLRGDPSVATGS"
     gene            596..1066
                     /locus_tag="AC16G0_04500"
     CDS             596..1066
                     /locus_tag="AC16G0_04500"
                     /inference="COORDINATES: protein motif:HMM:NF022112.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HIR5098917.1"
                     /translation="MAPSERWRVRLFFPQNSGDSLKEQERELTRQGNLTADVRTVLRA
                     LANGGGPGVLAPLPPGLEVRQAYLDAVGILYLDLGKGIQAVFGGPGTQPALALSATVT
                     TLTTSFSEVKRVQFLSEGQELNEVIGGVDLRRPVAPRFPGEEGPPITSEPQAAE"
     gene            1079..1816
                     /gene="rph"
                     /locus_tag="AC16G0_04505"
     CDS             1079..1816
                     /gene="rph"
                     /locus_tag="AC16G0_04505"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018130996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HIR5098918.1"
                     /translation="MRPDGRRPDEMRPVSITRNFIRHAEGSVLITVGDTKVICTASVE
                     DRVPEFLRKTGQGWVTAEYGMLPRSTKTRTQRESSAGRPSGRTFEIQRLVGRSLRGVT
                     DLPVLGERTIWMDCDVIQADGGTRTAAITGAFVALADALHTLRENGRIASFPLKDFVA
                     ATSVGVVDGQVVLDLCYAEDAVADVDMNVVMTGSGKYVEVQGTAEDRPFDRSLLDAML
                     QAATNGIGELIALQRKLLAERAAEAKR"
     gene            1831..>2264
                     /locus_tag="AC16G0_04510"
     CDS             1831..>2264
                     /locus_tag="AC16G0_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098919.1"
                     /translation="MQEQRPEGLDRPRHTGSLQWAAVVARAAIGIAWLTVFCLTVTGC
                     ATVGAADPHGRQAVIDRILAASAKVMIERDGKRVSSGSGVVVASRPLEPGVEAVSYVL
                     TAAHVLDGPEKAEVFVRFTGAFAVRGKLAATVERRGNPDTLDL"
BASE COUNT          401 a          693 c          779 g          391 t
ORIGIN      
        1 cgggagccat cggataacga cgcgcgtgcg tcggctcagc gcgagaactt gctgatcgac
       61 cgggacggaa ctacggggag ggatcaggaa tccctgctcc ggatcacgtt ggccgatttg
      121 gcggtcaccc gagatatccg ggaatcaggc gagcttgccg aactggcgct cagctccctg
      181 gataagattt tgaggatcga aaaccgcggc gtgaagtccg gcccgttcta cgtcctggcc
      241 accgtagcca tgcccgccgt tctcgtcgag agcgccttca tcaccaatcc taacgaggaa
      301 cggaagcttc agcgggacgc ataccgtcag cgcatcgcag aggcattgtg cgaggggatc
      361 ggcaaattca aggccaggta cgagcggcgg gtggggctgc ggggtgatcc ttcggtagcc
      421 acgggttcgt agcccaatgc cccggacggt ccgccccctc gcgctctggc catggttcag
      481 cctcctggtc gtcagccttg gagtgatcat tgctatcgtc tggtctgcct ggccctgggc
      541 tacgagcctt ccgagcgtaa agtcgcgtcg tgcggtgtca ccggtcccgg caggtgtggc
      601 accgagcgag cgctggcggg tccggctgtt ctttccgcag aactccggag acagcctgaa
      661 ggagcaggaa cgcgaattga cacgccaggg aaatctaacg gcggacgtac gaaccgtcct
      721 ccgtgctctg gccaacggtg gagggcccgg ggttctggct ccactgcctc ctggattgga
      781 ggtccgccag gcgtacctgg acgccgttgg aatcctgtat ctcgatttgg gcaaggggat
      841 tcaagcggtc tttggtggcc cgggcaccca gccggcgctg gcactctcgg ccaccgtcac
      901 cacactcacc acgagcttca gtgaggtcaa gcgggtgcag ttcctctccg agggacaaga
      961 attgaacgag gtgatcggag gagtagacct ccgccgtccc gtcgcacccc gttttcccgg
     1021 agaggaaggc ccgcccatca cgtccgaacc acaggcagcg gaatagcccg gaggacgcat
     1081 gcgaccagac ggacgtcgcc cggatgagat gcgcccggtg tcgatcaccc ggaatttcat
     1141 ccgacacgcg gagggatcgg tcctgatcac ggtgggcgac accaaggtta tctgcacggc
     1201 cagcgtggaa gaccgagtcc ccgagttcct gcgcaagact ggacagggat gggtgacggc
     1261 cgagtacggg atgttgcccc gctccacaaa gactcggacg cagcgggagt cgtcggcggg
     1321 tcggcccagc ggccgcacgt ttgagatcca gcgtctcgtc gggcgttcgc tgcgtggcgt
     1381 gaccgacctc cctgtcctgg gcgagcggac catctggatg gactgcgacg ttatccaggc
     1441 agacgggggc acgcgcaccg cagccatcac cggggcattc gtggccctgg ctgacgccct
     1501 ccatactttg cgggagaacg gtcgaatcgc cagcttcccc ttgaaggact tcgtggcggc
     1561 caccagcgtc ggcgtcgtgg acggccaggt ggtcctggat ctgtgctatg cagaggatgc
     1621 tgtggccgat gtggatatga acgtggtgat gacgggctcg gggaaatacg tcgaggtcca
     1681 agggacagcc gaggatcgcc cgtttgatcg cagcctgctc gatgccatgc tgcaggcggc
     1741 cacgaacggc atcggggaac tcattgccct ccagcgaaag ctgctggccg aacgggcggc
     1801 cgaggccaag cgatgacgcc tggagatgac ttgcaggagc agaggccgga gggtctggat
     1861 cgccccaggc acacgggcag cctccagtgg gctgccgtcg tggcgcgcgc agcaattggg
     1921 atcgcctggt tgacggtctt ctgcctgacg gtcaccggat gcgccaccgt cggcgccgcc
     1981 gatccgcatg ggaggcaggc cgtcattgat cgcatcctgg cggcgagcgc gaaggtgatg
     2041 atcgagcgcg acggcaagcg tgtgagttcg ggctcgggcg tcgtggtggc ctcacggccc
     2101 ttggaaccgg gagtcgaggc cgtctcctac gttctgactg ccgcccatgt cctggacggc
     2161 ccggagaagg ccgaggtctt cgtcagattc accggggcct tcgccgtccg gggaaagttg
     2221 gccgcgacgg tcgaacggcg aggaaatccc gacaccctgg atct
//
LOCUS       DBNGZC010000247         3599 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2074288, whole genome shotgun sequence.
ACCESSION   DBNGZC010000247 DBNGZC010000000
VERSION     DBNGZC010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2074288"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="AC16G0_04515"
     CDS             <1..249
                     /locus_tag="AC16G0_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5098920.1"
                     /translation="ATAVISFALAWSEFMFAFILTATPRSQTFPIGVASLVTQFEIIW
                     NEMAAVGSMAAAVPILLLLFARRYVITALTFGAVREKA"
     gene            599..790
                     /locus_tag="AC16G0_04520"
     CDS             599..790
                     /locus_tag="AC16G0_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098921.1"
                     /translation="MRTVVSHFWIHPPDGEPTLYEVDYSVYPIRLGVLCYAKIPRSER
                     PCTCPERNRQARQLVAALN"
     gene            814..987
                     /locus_tag="AC16G0_04525"
     CDS             814..987
                     /locus_tag="AC16G0_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098922.1"
                     /translation="MCCEHCGSEMAFTFYHKTCRVWWCRFCTELFLEDSGHQYLLNPG
                     QASETRLIEHRGV"
     gene            complement(1248..1439)
                     /locus_tag="AC16G0_04530"
     CDS             complement(1248..1439)
                     /locus_tag="AC16G0_04530"
                     /inference="COORDINATES: protein motif:HMM:NF014470.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HIR5098923.1"
                     /translation="MAKLVVVLALALIVLGPQDLPRLSLSLLRTVEEFRRTLEGFSKS
                     LRRELELLERDEATHPPNI"
     gene            complement(1525..2709)
                     /locus_tag="AC16G0_04535"
     CDS             complement(1525..2709)
                     /locus_tag="AC16G0_04535"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5098924.1"
                     /translation="MAHPLRVLILEDNASDAELMIHDLRRAGFDPSWRRVETEAAIVA
                     ALDGDPEVILADHSLPGFDVFRALRHLRERMLDIPCLVITGTVGDEAAAECIKQGACD
                     YLLKDRLARLGGAVLGALEQRRLGREKRLAENRLRERSEQLRAMAAELTLAEPRERRR
                     LAEVLHDGIQQLLVGAKLRVQPLDRVADAQVREAGREVAGLLQEALEQTRSLTRELSP
                     PILHAGGLIPALEWLGGWMAEKHGLKVVLGIEGTAGLKLEENLSILLFQSVRELLFNV
                     VKHAKVQTARLEIKEQDKYVHIQVSDAGVGFDPARLQVEGGKAGSFGLFSIRQRLELL
                     GGYLCIDSASGQGSRFALSAPLAGAQAVLRSAPTASGVPPRTTIQPGEGHLGLTGEPG
                     DV"
     gene            complement(2826..3233)
                     /locus_tag="AC16G0_04540"
     CDS             complement(2826..3233)
                     /locus_tag="AC16G0_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015188598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5098925.1"
                     /translation="MAENPVEILLVEDNPNDVELTLHALKRNHLTNRVHIVRDGAEAL
                     DFLFGSGSSPDRPIQETPRVILLDLKLPKVDGLEVLRRVKGDPRTRMIPIVVLTSSRE
                     ERDVVESYRLGVNSYIIKPVDFEQFTEAVRQVG"
BASE COUNT          640 a         1179 c         1123 g          657 t
ORIGIN      
        1 gccacggcgg tgatcagctt cgccctggcc tggagcgagt tcatgttcgc cttcattctt
       61 accgccaccc cgcgctcaca gaccttcccc atcggcgtgg cgagcctggt cacccagttc
      121 gagatcatct ggaacgagat ggcggcggtc ggctcgatgg ctgccgccgt gcccatcctg
      181 ctcttgcttt tcgctcgccg atacgtgatc acggcgctga ccttcggagc cgtgcgtgag
      241 aaggcgtaag ggcgagccga gggcccaggc caagcacgcg gggcccggct ggcctcttgc
      301 ctctgatcct gctgctatcc tggtaaccca gatttgccct tggatcccct ggcaggccgt
      361 ccttcttctc cccggactgg attctgtagg acaagctgcg acgcccacca gagtggaaat
      421 ctgacgaaaa attcagcgtt tggctgatta ccagggggtg ggccgcgtgg tatctatgcc
      481 cacagatgag gaacctactt cgggcccgag gaatctaggc gggcagaccc caaaactccc
      541 gcccattgga agaggctgaa cctgaaccag cgcgagaggg cgaaggaggc gattgccaat
      601 gagaacggtc gtgagccact tctggatcca ccccccggac ggcgaaccca ccctgtatga
      661 ggtggattat tccgtctacc cgatccggct gggggtgctg tgctatgcga agatcccccg
      721 ctcggagcgc ccctgcacct gtccggagcg gaataggcag gcgcggcagt tggtggccgc
      781 cttgaactga aaccgaaggg agatgagggg aggatgtgtt gtgagcactg tggaagcgag
      841 atggccttca ccttctacca caagacctgc cgggtctggt ggtgtcgatt ctgcaccgag
      901 ctctttctcg aagacagcgg gcaccagtac ctgctcaacc cggggcaggc ttccgagacg
      961 cggctcattg aacaccgggg ggtctaaggg ggagacccca aaggggcccg cgtgggacga
     1021 ctcgccgacg cacgtcatta cctgtcgctg ttgcgagacg accgtccagg tccccatccg
     1081 gcgccgattc ctctgcgacg actgcttcca ggctggcgcc tgaggaggta ttcccggccc
     1141 ggcggcagcc gacgggccga cccatcgggc ggatctggcc tggcaatact accgggccgt
     1201 gaggctgctg cgctctcaga ggcaagctgc cgaaaagggg cccgtctcta aatattcggc
     1261 ggatgggttg cctcatcccg ttccaagagc tccagctctc tacggagaga tttggagaag
     1321 ccctccaatg ttcggcgaaa ttcctccacc gtccgcaaga ggctgaggga gagcctggga
     1381 agatcctgcg gcccgaggac gatcagggcc agggccagaa ccaccacaag tttagccatt
     1441 caccctcctc ctgcgtgggg cgatccggaa gccagagcgt ccctgctcct gcgagccagg
     1501 tatccctttc ctgaactgac cggattatac gtcgccaggc tcacccgtca aaccgagatg
     1561 tccctccccc ggctggatcg tggttcttgg cggcaccccg gaagcagtgg gggcggaccg
     1621 gagcacggcc tgagcacctg cgagcggggc cgagagcgca aaccggctgc cctgcccgga
     1681 ggcgctgtcg atgcacaggt atcctcccag aagctccagg cgctgccgga tgctgaacag
     1741 gccgaagctg ccggccttgc ctccctccac ctggaggcgt gcggggtcga aacccacgcc
     1801 cgcgtccgag acctggatgt gcacatactt gtcctgttcc tttatctcaa gccgtgctgt
     1861 ctgcactttc gcatgcttca ccacgttgaa caggagttcc cgcacggact gaaacagcag
     1921 aatactcagg ttctcttcca gcttcagccc ggcggtccct tcgatcccca agacgacctt
     1981 caggccatgc ttctccgcca tccagccgcc cagccactcc agggcgggga tcaggccccc
     2041 ggcatggagg atgggcggac tgagttcccg ggtgagggat cgagtctgtt cgagtgcctc
     2101 ctgcagaagc ccggccacct cccgacccgc ctcgcgcacc tgggcatccg cgacccggtc
     2161 gaggggctgc acccggagct tcgccccgac cagcaactgc tggattccgt cgtgcagcac
     2221 ctcggccagc cgtcggcgct cccgcggctc ggctagtgtc agctccgcgg ccatggcacg
     2281 gagttgctcc gagcgctccc gcagcctgtt ctccgccagc cgtttttctc tccccagacg
     2341 gcgctgttcg agcgccccga gcaccgcgcc gcccagccgg gccagccggt ccttcagcag
     2401 ataatcgcag gcgccctgct tgatgcactc cgcggccgct tcgtccccga cggtccccgt
     2461 gatcaccaga caagggatgt ccagcatgcg ctcccggagg tggcgcaggg cccggaagac
     2521 gtcaaacccc ggcagcgagt ggtccgcgag gatcacctcg gggtcgccgt cgagtgcggc
     2581 gacgatggcg gcctcggtct ccacccgtct ccaggacggg tcgaagcccg cacggcgcag
     2641 atcgtggatc atgagctcgg catcggatgc gttgtcttca agaatcaaga cccgcaatgg
     2701 atgggccatg aggctcccgc cttcccttgc tcttcgaggg acgatcggtg cggcagttgg
     2761 agtctgctat ctcccggtcg gaaccattag atggtccgcg gcggctcgtt cagaagcagc
     2821 cagtattatc ccacctggcg cacggcttcc gtaaactgtt caaagtccac gggcttgatg
     2881 atgtagctgt tcacccccag ccggtaactc tccaccacgt cgcgctcctc acgcgaggag
     2941 gtcaggacca cgatggggat catccttgtc cgggggtcac ccttgactcg acggagcacc
     3001 tccaagccat ccactttggg gagcttgaga tcgaggagga tcactcgggg tgtctcctgg
     3061 atgggccggt ccggggacga cccgctgcca aaaagaaagt ccagagcctc tgcgccatcc
     3121 cggacgatgt ggacccggtt ggtgaggtga ttccgcttga gggcatgcag ggtaagctcc
     3181 acgtcattcg ggttatcctc caccagcagg atttcgactg gattctccgc catgcgagag
     3241 cctcctggat gagttggtga aacctgaacc gtatggtacg cagcgcccgg catggtacag
     3301 ggggtctggg ttcgccaggg ctgcaggtcc ccggtcgccg ccttctccag tgtgattcca
     3361 ggtgccaaac gcggccgtga ccaattcctg tccgccgttg tgccgcgggt aatatatcgc
     3421 cacgctggtg gagacctcac aactctcgag cagaaagcgc ttggaactca gacaagccca
     3481 gagtcgccct gcggggctat gcatgtcaag ctgcagcgca gccatgtaag gaggctggga
     3541 caatggggcg gatggctccc tgaaggggac agggacgctc cggtggtccg atacgctcg
//
LOCUS       DBNGZC010000248         2542 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1292189, whole genome shotgun sequence.
ACCESSION   DBNGZC010000248 DBNGZC010000000
VERSION     DBNGZC010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1292189"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /gene="tcuB"
                     /locus_tag="AC16G0_04545"
     CDS             complement(<1..1057)
                     /gene="tcuB"
                     /locus_tag="AC16G0_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tricarballylate utilization 4Fe-4S protein TcuB"
                     /protein_id="HIR5098926.1"
                     /translation="MRICNACRYCEGYCPVFPALERRLTFEESDLNYLANLCHNCGSC
                     YDACQYAPPHEFALNFPKILAEIRRETYKKYAWPGFLAGLFRRNGLAVSLITGAGVAI
                     FLLALTVFAGPAVLLSAHDLRQGSFYAVLGHDAMALTFSLVSLLVASAFVVGFVRFWR
                     DIGERFDAILNPMVLGRAAMDVLRLKHLDGGGEGCIYPDEEPTLVRRRFHHLTFYGFL
                     LCFASTTVAAFYDYVLRWPAPYAFWSLPVLLGTLGGLGLLIGPAGLLWLKVKRNPVLS
                     DQEQTGMDIGFLALLFLTSLTGLLLLALRETPAMGVLLAVHLGLILGLFVTMPYGKFV
                     HAIYRAAAIVRYAIEESR"
     gene            complement(1098..2492)
                     /gene="tcuA"
                     /locus_tag="AC16G0_04550"
     CDS             complement(1098..2492)
                     /gene="tcuA"
                     /locus_tag="AC16G0_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HIR5098927.1"
                     /translation="MDVSRTYDVLVLGGGNAALTAALTARRAGLSVLVLECAPKDFRG
                     GNSRHTRNLRCMHDRPVDVLTDAYPEEEYWKDLLRVTGGKTNEALARMTIQQSADCRA
                     WMVEQGVRFQASLRGTLHLQRTNAFFLGGGKALVNAFYQTARALGIEIEYEAEVQDLT
                     IQDGRFTSATVVQRGATYEVRAKTVVAASGGFESNLAWLKEVWGEVADNFLVRGTPYN
                     MGRVLRVLIDRGAKAVGDPTQCHAVACDARAPKFDGGIITRVDAISLGIVVNKHAERF
                     YDEGEDFWPKRYAIWGRLVAGQPDQKAFAVIDAKPMGSFIPPVYPPIQASSIRALAPA
                     LDLDPESLEETVTRFNRAVRPGRFDHAVLDDCTTVGLTPPKSHWARPLDTPPFFAYPL
                     RPGITFTYLAVTVDDRARMIMQDDTPATNIFAAGEIMAGNILGKGYLAGIGMTIGTVF
                     GRIAGQEAVRYVRG"
BASE COUNT          495 a          827 c          794 g          426 t
ORIGIN      
        1 ggcgcgactc ctcgattgca taacgcacaa tcgctgcggc gcgatagatg gcgtgcacga
       61 acttgccgta aggcatggtg acgaagaggc ccaggatcag cccgaggtgg accgccagca
      121 gcacgcccat ggccggtgtc tcgcgcagtg ccaggagcag caagcccgtg aggctcgtca
      181 ggaataacag cgccagaaat ccgatatcca tccctgtctg ctcctggtcg gacaaaaccg
      241 gattccgctt gaccttcagc caaagcagcc ccgccggtcc gatcaagagc ccgagaccgc
      301 cgagggttcc cagcaggacg ggcaggctcc aaaatgcata cggggctggc cagcgcaaca
      361 catagtcgta aaatgcagcc acagtggtcg aggcaaagca cagcaggaac ccgtaaaatg
      421 tcaggtgatg gaaccgtcgg cggaccagcg tgggctcctc atccggatag atgcagccct
      481 ccccgccccc gtcgaggtgc ttgagccgca gcacgtccat cgccgcccga ccaagcacca
      541 tgggattcag gatcgcgtcg aatctctctc cgatatcacg ccagaagcgt acaaatccca
      601 cgacaaacgc cgaggcgacg aggagggaaa cgaggctgaa ggtcaacgcc atggcgtcgt
      661 gacccagcac ggcatagaag ctgccctggc ggagatcgtg ggccgagagc aacaccgccg
      721 gccccgcgaa gacggtcagc gcaagcagga agatcgccac acctgcccca gtgatgaggc
      781 tgactgccag cccattgcga cggaacaagc cggccaagaa gccgggccag gcgtatttct
      841 tgtaggtttc cctgcggatt tccgccagga tcttcgggaa gttcagcgcg aactcatggg
      901 gtggggcgta ttggcatgcg tcatagcacg atccacagtt gtggcagagg ttggcgagat
      961 aattcagatc gctctcttcg aatgtgagcc gtcgctccag cgccggaaag accgggcagt
     1021 atccttcgca gtagcgacag gcattgcaga tcctcatgaa ccactcgccc tgctggacca
     1081 gttcgagagg aattgattta gccgcggaca tatcgaaccg cctcctgtcc ggcgatccgc
     1141 ccgaagacgg tgccgatggt catcccgatc ccggcgaggt agcctttgcc taggatattg
     1201 cccgccatga tctcccccgc cgcgaagatg ttcgttgccg gcgtatcgtc ctgcatgatc
     1261 atccgggccc gatcgtccac cgtgacggcc agataggtga aggtgatgcc ggggcgaagc
     1321 ggatacgcaa agaagggcgg cgtatccaac gggcgggccc agtggctctt cggaggggtc
     1381 aacccgacgg tggtgcagtc gtccaggacg gcgtggtcga accgcccggg ccgcaccgcg
     1441 cggttgaacc gtgtgaccgt ttcctcgagg ctctcggggt cgaggtcgag ggccggggcc
     1501 agtgcccgga tcgacgacgc ttggatcggg gggtagacgg gaggaatgaa gctgcccatc
     1561 ggcttcgcgt cgataacggc gaatgccttc tgatccggct gcccggccac gagccgtccc
     1621 cagatggcgt agcgcttggg ccagaagtcc tccccctcgt cgtagaaacg ctcggcatgc
     1681 ttgttgacca cgatcccgag ggaaatcgcg tccacccgcg tgatgatgcc cccatcgaac
     1741 ttgggggccc gggcgtcgca cgccactgca tgacactggg tcgggtcgcc cacggctttt
     1801 gccccgcgat cgatcaagac gcgcagcact cgacccatgt tgtacggggt gccgcggacc
     1861 agaaagttgt cggcgacctc tccccagacc tccttcaacc acgccaggtt cgactcgaac
     1921 ccgccggagg ccgccaccac cgtcttggcc cggacctcgt acgtggcccc gcgctgcacc
     1981 acggtcgcgg aggtgaatcg gccgtcctgg atggtgaggt cctggacctc cgcctcgtat
     2041 tcgatctcga tgcccagcgc ccgcgcggtc tggtagaagg cgttcacgag ggccttgccg
     2101 ccccccagga agaaggcgtt cgttcgctgg agatggaggg tcccgcggag cgaagcctgg
     2161 aagcggacgc cctgctccac catccaggcc cggcaatccg ccgattgctg gatggtcatc
     2221 cgtgccagcg cctcgttcgt cttccctcct gtcacccgga gcagatcctt ccagtactcc
     2281 tcttccgggt aagcgtccgt cagcacgtcg acaggacggt cgtgcatgca acggaggttc
     2341 cgcgtgtgtc gactgttccc gccgcgaaag tccttgggag cgcactcgag cacaagcacg
     2401 ctcagtccgg cgcggcgggc cgtcagggcg gccgtgagtg ccgcatttcc gccgcccagg
     2461 accaacacat cataagtgcg tgacacgtcc atcaattctc cccttcctct cgcgtcaaaa
     2521 ccggtacctg gtgctgggaa tg
//
LOCUS       DBNGZC010000249         2216 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_411465, whole genome shotgun sequence.
ACCESSION   DBNGZC010000249 DBNGZC010000000
VERSION     DBNGZC010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_411465"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="AC16G0_04555"
     CDS             complement(<1..505)
                     /locus_tag="AC16G0_04555"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR5098928.1"
                     /translation="MQMRAARYHGPGKPLSLETVQVRPLTAGEALVRVKAAGICYTDT
                     HFLSGVLNLGIQPLTLGHEIAGEVAELGKEARGVAVGDRVLVYYYVTCGHCHWCRTDL
                     ANLCPQVVAEYGFTADGGYAEYVVVPASNLVKIPAGLDLKEAATLGCSVTTAIHAART
                     IADLRLGE"
     regulatory      complement(563..676)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(782..1105)
                     /locus_tag="AC16G0_04560"
     CDS             complement(782..1105)
                     /locus_tag="AC16G0_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019550317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5098929.1"
                     /translation="MQILAVAGRARFADDKMQKIGLIETDRLYFDLYCLKPGQAQKVH
                     SHVGSDKIYYVLSGRAAVRIGEEEAELTPGQAVLAPSGCLHGVANRGGEPLSLLVFMA
                     PKPSH"
     gene            complement(1228..2130)
                     /locus_tag="AC16G0_04565"
     CDS             complement(1228..2130)
                     /locus_tag="AC16G0_04565"
                     /inference="COORDINATES: protein motif:HMM:NF021287.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HIR5098930.1"
                     /translation="MTEPSSSLVFEEFPQDLRRPILVAAFAGWNDAAESATSAARFLC
                     QRWSGRRFARILPEEYFHFGLTRPEVRIQEGSQVREIQWPANDFFIGVEPSLPRDAIA
                     CIGIEPHLRWQAFCGRILEICRRAQVTTVITLGALLADVPHTRPIRVTGVATDPELAR
                     RLQTSRTRYEGPTGIVGVLNDACRKAGLPVISLWANVPHYVSEVSNPHAILALVRRVC
                     DFLEWRPDLSELEEARVEFDRQLARVLSEKPDVARYIEELERRESEAEASAEPSASDL
                     PNSGELIREVEKFLREHRGDRGPA"
BASE COUNT          391 a          768 c          663 g          394 t
ORIGIN      
        1 tctcgcccag ccgcaggtcg gcgatcgtcc gcgccgcgtg gatggctgtg gtgacactgc
       61 acccgagtgt cgccgcttct ttgaggtcca agccggcggg aatcttgacg aggtttgacg
      121 cgggcacgac cacgtactcg gcgtagccgc cgtcggccgt gaacccgtac tccgccacaa
      181 cctgcggaca aaggttcgct aggtctgtgc ggcaccagtg acagtggcca caggtgacat
      241 agtaatacac gagaactcga tcgccgaccg caaccccccg ggcctctttc ccgagctctg
      301 ccacctcgcc cgcgatctcg tggcccaggg tgagcggctg gatccccagg ttcaggaccc
      361 cgctcaggaa atgggtgtcg gtatagcata tccccgcagc cttcacccgg accagcgcct
      421 cacccgccgt cagggggcga acctgcactg tctccagact cagcggcttt cccggaccgt
      481 gatacctggc tgcacgcatc tgcatcccgg tctctcctcc agtcgaccga tactcaaaca
      541 aaaaaccgcc acgatgggcg gtttgtcttt actttttgat ccctacgctg gcattaccca
      601 ggtcaggttc aaacggtcgg tgagggaacc gccccaccct ctcagcccgg caccccgggc
      661 tcccgtattt attggctgaa ctgtatcacc cttctatatg ctcgtcaaga cacgggacgt
      721 cccggtgggg ctgaaccctc cacgagagcc tgctcgacgt ccgcgctgcc gtgggctggg
      781 gctaatgcga cggcttgggc gccatgaaca ccaggaggct gaggggttcc ccgcctcgat
      841 tggccacgcc gtgcaggcat ccggaggggg ccagaactgc ctggccaggc gtcagctccg
      901 cttcctcctc accgattcgt accgcagccc gaccgctcag gacatagtag atcttgtccg
      961 agccgacatg gctgtgcacc ttttgggcct gaccgggctt gaggcagtac aggtcgaagt
     1021 acaatcgatc ggtctcgatg agaccaatct tctgcatctt atcgtcggca aagcgtgccc
     1081 tgccggcgac cgccaagatc tgcatgagag gcccctcgct ttcgccagcc ggtcagcatc
     1141 aatccgaaaa accgttcaac ccccatggct gtgcttggca ccctggcagt gagacattcc
     1201 ggagaaaggt tcggaaaggc tccggcgtta ggccggccct cgatccccgc ggtgctcccg
     1261 caggaacttc tccacttccc ggatcaactc gccgctgttg ggcagatcac tggcggacgg
     1321 ctctgccgac gcctcggcct cgctctcccg ccgctccagc tcctcgatat accgggcgac
     1381 atccggcttc tccgacagga ctcgcgccag ttgccgatcg aactccaccc tggcctcttc
     1441 cagttcgctc aggtccggcc gccattccag gaagtcgcac acccgccgca ccagtgccag
     1501 gatggcgtga gggttgctca cctctgagac gtaatgagga acgttggccc acaggctgat
     1561 aaccggaagg cctgccttcc gacaggcatc gtttaacaca cccacaatcc ccgtcggccc
     1621 ctcgtaccgt gttcgactcg tttgcagacg ccgcgcgagt tccggatcgg tggcgacacc
     1681 ggtcacccgg atgggccggg tgtggggcac gtcagccagg agtgccccca gggtgatcac
     1741 ggtcgtcacc tgggcccggc ggcagatctc caggatgcgg ccgcagaagg cctgccatcg
     1801 gaggtggggc tcgatcccaa tgcaggcgat cgcgtctcgc ggcagggacg gctccacgcc
     1861 tatgaagaag tcattggccg gccactggat ctcccgcacc tgggagccct cctgaatccg
     1921 cacctccggc ctggtgaggc cgaagtggaa atattcctcg ggcaggatgc gagcgaagcg
     1981 gcggccggac cagcgctggc agaggaagcg ggccgccgag gtcgcgctct ccgcggcgtc
     2041 attccagcct gcgaaggccg ccaccaagat cggtcggcgg aggtcctgcg ggaattcctc
     2101 gaagaccagg gacgaggaag gttcggtcat tgcatatccc ctcggctagc aggtttgtca
     2161 gaggaaccac tcagatcggc ggcgcgcccg gtcccccccc acccaccccc gcgcgg
//
LOCUS       DBNGZC010000250         7409 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1292332, whole genome shotgun sequence.
ACCESSION   DBNGZC010000250 DBNGZC010000000
VERSION     DBNGZC010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7409)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7409
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1292332"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="AC16G0_04570"
     CDS             <1..199
                     /locus_tag="AC16G0_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098931.1"
                     /translation="DARVQAAVEQIRTAAAAAGKPAMIVALDPADGRALYTQGFQVLM
                     VSATSLLVNAAGTFLKDIARG"
     gene            complement(236..526)
                     /locus_tag="AC16G0_04575"
     CDS             complement(236..526)
                     /locus_tag="AC16G0_04575"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR5098932.1"
                     /translation="MPTVTVRTMFDLARIVGQPTVKVTVGAGATVRTALEELTTAHDG
                     LRGRLFDPGTGNLPPYLFIELNGRDIRILQGLETPVGDGDALFVAIPVAGGA"
     gene            complement(548..2416)
                     /locus_tag="AC16G0_04580"
     CDS             complement(548..2416)
                     /locus_tag="AC16G0_04580"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR5098933.1"
                     /translation="MMMGKILRVNLTSRQITETPYPEVLARQFMGGAAAAIKILYDEV
                     DPKVDALGPDNKLIVSMGFLAGSSAPVTSRVAFVAKSPLSGTVGYSNSGGYFPNELRA
                     TGVQAIILEGKAEAPVYLWIGDDKAEIRDAKDYWGLSTIDTQYYMQQALHDGNIRVAC
                     IGPAGEHLVKYAAIIHERRASGRKGLGAVMGAKNLKAIAVRGSQKLPPLADPEAFKEA
                     TKILHAAMKESPALYHRFRNFGTAGNVELTVGQGDMPAKNFQEPAGEEYLAIGGQVGT
                     GYRVRHFACDQCPVQAAQVRAVSSGPYAGYASEGPEFESLYSLGSDMGITDMGACIAM
                     DRLCDELGIDTVSFGATVAMAMELYERGILKRHEVDGLDLKFGNHEAAVAVLRKVAYR
                     EGVGDLLAEGSKLAAQRIGGDAPKYAMHVKGLEFPGYDPRGLKSMGLNYMTAFTGADH
                     SRGHSEQEVFGIPVPHAVDRLAIEGKAALTIFNQDVGASVKDSAMLCTFVVRLALLPK
                     NLGPKLVSDFWRAAQGYDVTPEEVVRVGERMTNVARIWNLRAGFTRADDTLPERLLME
                     PLQTGASKGHRISPEDRDFMLNEYYSLHGWDVRGVPTPGRLAALGLEALVADLPRP"
     gene            3157..3948
                     /locus_tag="AC16G0_04585"
     CDS             3157..3948
                     /locus_tag="AC16G0_04585"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR5098934.1"
                     /translation="MRPRTHERGGTVQSVERALTLLEALGDCRGEVGIANLSKRVGLH
                     VSTVHRILGTLLERGYARQNPETGHYGLGAKTFSLAESYLGQMDLSRLVRPALERLSR
                     ETEETANLVILDGGEALYLDKVESPQNLRIFSRIGHRVPLHCSAVGKVLLASRAQKDA
                     DALLGRRPLEGLTRSTITSRVQLRRELEKVREQGFALDCEECEEGACCIAVPLKDAQG
                     RVEASLSVSGPSVRLTPKRMEELIPLMRQVAREVSSQLGCNTPEG"
     gene            4001..5749
                     /gene="gcl"
                     /locus_tag="AC16G0_04590"
     CDS             4001..5749
                     /gene="gcl"
                     /locus_tag="AC16G0_04590"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HIR5098935.1"
                     /translation="MPKMPVMEAAVKIMESEGVEVCFGIPGAAILPLYKALTKSSKIR
                     HLTVRHEEGATHAADGWARITGKVGVAIGTSGPAGTNMVTGLYTCIGDSIPIICITGQ
                     ALTERLHTEAFQAVDIAEIAKPVTKKSYCVKETAQIPQVFREAFRIAREGRPGPVLID
                     LPLNVQRGEVEYDPDIDAPLEVFKPSPDARKIRRAIEMLLQAKRPILMMGGGVMIARA
                     SREFVALAEHLQIPVIPTYMAEGGIAFDHPLYGGRVGLQTNTRAGNKLFLESDLVLSI
                     GNRFAERHTGDLTVYRGERKFIHIDIEPTVIGKVLPADLGIVSDAKLAVEALMRVAKE
                     MTPKRSPNDWSKKAADYKRRFRRKMDFDQVPVKPQRVFKEINEFFDSDTIFVTAIGLY
                     QIWSGQFQEIKNPNTYFCCGQAGPLGWEVPACMGVKLGKPDAQVVGVVGDYSFQFLLG
                     DLATAVQNNVPYVIVMLNNGYLSLIRQPSKYLYHMNYGVDIGYGDGYGPDFVKIMEGF
                     GGAGKRVLKPEEIKPALAWAVQESNNRKVPVLVEIRVDRETDAAMGVSIDKIIERDPV
                     IDLPEEAPVKEPVSVR"
     gene            6006..6905
                     /locus_tag="AC16G0_04595"
     CDS             6006..6905
                     /locus_tag="AC16G0_04595"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5098936.1"
                     /translation="MAERIGFLGPGLMGKGIVKNLLKKGFPVTVFAHRVGLNLNELKQ
                     AGASVTTSLAEVGETSERVLLCVPSSKEVEAAVLGTPGLLQSMRKGSVIIDLSTSYPA
                     STCMLAERLAAKGIAFLDSPMTGTPVHANAGELNIMIGGDKAVYEQCLPIFQAIAKNI
                     FHVGPSSHGNIVKLINNFLGQLTNAGLAEVLPLAVKSGIDINALYDVVRVSGGNSRVF
                     EGSVPAICKRDFTVTFEIRLAHKDMNYMSSLGREMNVPLPMVNSLLNVLDVAKASGLA
                     RENTNALVKMWEKVSDVVVKGWK"
     gene            7207..>7409
                     /locus_tag="AC16G0_04600"
     CDS             7207..>7409
                     /locus_tag="AC16G0_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098937.1"
                     /translation="MKQSWSLAGLLAVALGLVLGPGFAADIRAAEFDLVEAHTTPPDH
                     PYTLGMVHYAQLVKERSRGRVAI"
BASE COUNT         1355 a         2359 c         2371 g         1324 t
ORIGIN      
        1 ggatgcccgc gtgcaggcgg ccgtcgagca gatccggact gccgcggcgg cggccggcaa
       61 accggccatg atcgtcgccc tggaccccgc cgacgggcgc gccctctaca cgcagggctt
      121 ccaggtgctc atggtcagcg cgacaagcct cctcgtcaac gccgcgggca ccttcctgaa
      181 agacatcgcc agaggctagc gcgggccgag gccaagcaag cacacctggc gccgatcatg
      241 cgcccccggc cacggggatc gccacgaaca gggcgtctcc atctcctacc ggggtctcca
      301 gtccctggag aatgcgaata tcccgcccgt tcaactcgat gaagagatac ggagggaggt
      361 tgccggtccc gggatcgaac aggcgacccc gcaggccgtc gtgcgcggtc gtcagttctt
      421 ccagggcggt ccgcacggtc gcacccgccc cgacggtgac cttcaccgtg ggttgcccca
      481 cgatccgggc gaggtcgaac atcgtgcgga ccgttaccgt cggcatgcca ccccctgttc
      541 ccgcgtatca agggcgcggc agatccgcca ccagggcctc caggcccagg gccgccagcc
      601 gccccggcgt cggcacccct cgaacgtccc agccgtgcaa gctgtagtac tcgttcagca
      661 tgaagtcccg atcctccgga gaaatgcggt gtcccttgga ggcgccggtc tggagcggct
      721 ccatcagcag gcgctccggc aacgtgtcgt ccgcccgcgt gaagccggcc cgcaggttcc
      781 agatgcgggc cacgttggtc atccgctcgc ccacccgcac gacttcctct ggcgtcacat
      841 cgtatccctg ggccgcccgc cagaaatcgg agaccagctt gggcccaagg ttcttcggca
      901 gaagggccag ccgaacgacg aaggtgcaga gcatggcgga atccttcacg gaggcaccca
      961 catcctggtt gaagatggtg agggcggcct tcccttcgat ggcgaggcga tcgacggcgt
     1021 ggggcacggg gatgccgaac acttcctgct ccgagtgtcc acgcgagtga tccgcgcccg
     1081 tgaaggcggt catgtagttg aggcccatgg acttgagccc gcgcggatcg tagcccggga
     1141 actccagccc cttcacgtgc atggcgtact tgggagcgtc cccgccgata cgctgggcgg
     1201 ccagctttga tccctcggcc agcagatcgc ccacaccctc gcggtaggcg accttcctga
     1261 ggaccgccac cgccgcctcg tggttgccga acttcaggtc gaggccgtcc acctcatgcc
     1321 gcttcaggat gccccgctcg tagagttcca tggccatggc cacggtggcg ccaaaggaga
     1381 cggtgtctat ccccagctcg tcgcaaaggc gatccatggc gatgcatgcg cccatgtcgg
     1441 taatgcccat atcgctcccc agggagtaga gcgactcgaa ctcgggcccc tcgctggcgt
     1501 aaccggcgta cggacccgaa gatacggcgc gtacctgggc ggcctggacg gggcactggt
     1561 cgcaggcgaa gtggcggacc cgatagccgg tgcccacctg gcctccgatg gccaggtact
     1621 cttcgccggc cggctcctgg aaattcttcg ccggcatgtc gccctggccc acggttagct
     1681 ccacgttccc cgcggtgccg aagtttcgga atcggtggta gagggccggg ctctccttca
     1741 tggccgcgtg gaggatctta gtcgcttcct tgaaggcttc cgggtcggcc aggggcggca
     1801 gcttctggct tccccgcacg gcgatggcct tcaggttctt ggcccccatc accgcgccga
     1861 gccccttccg cccggaggcc cggcgctcgt gaattatggc agcgtatttc actaagtgct
     1921 cgccagccgg cccgatgcag gccacgcgga tgttgccgtc gtggagcgcc tgctgcatgt
     1981 agtattgcgt gtcgatggtg ctcagccccc agtagtcctt ggcgtcgcgg atctccgcct
     2041 tgtcgtcgcc gatccagagg tacacgggag cctcggcctt gccctccagg atgatggcct
     2101 gcacgccggt tgcccgaagt tcgttgggga aataccctcc cgaattcgaa tagcccacgg
     2161 tgccagagag gggggacttg gccacgaagg ccacgcggct cgtgaccggc gccgaactcc
     2221 cggccaggaa gcccatactc acgatcagct tattatccgg ccccaaagcg tccaccttgg
     2281 ggtcaacctc gtcatagagg atcttgatgg ccgcagccgc cccacccatg aactgtcgcg
     2341 cgaggacctc ggggtacggg gtctcggtga tctgcctgct cgtcagattc acccgcagga
     2401 tctttcccat catcatgacg cttcctcctc tcctcatccc ccatcccgtt gcgtttgtca
     2461 cgaagcggta ccggacgcgc ccgcatgctt cgcgcctcag cccctggcct tcctgctgca
     2521 tgcgaaccgc atggccgggc acattatgct taccgtgtcg accgcgtgta ccacacggct
     2581 gaagtccggg tgtgcgcggc tcgcaccggg aagcctgatg cgagccgcag ctcattcccc
     2641 cagcttcatg aacacgggaa atcgagtgtc actgaccgct ccctccaggg aaatgttcgc
     2701 cccggaagaa cgacaaccct ctggatatga gtctccaaat ggctgtcaga ctcgcaggcc
     2761 aggagttttc cagctaccat ggctgccccc tcgatcgcgt accgggtagc atcccgaaat
     2821 ggtagcacga ggctatctct gtctcgaaag tgaagtcaag gtggcaaaga cggctgtatg
     2881 tccttacagt caacatgggc gacactcggc ggccctgatg gcaagtcgtg tcgatagggc
     2941 ggtctccact ggctacggag acgccggcat ttccagcact caagatcatc gttttctggt
     3001 caggaagcgc ttgacaagat ccggtacctc cgctacgttc acatcacgga actaattatc
     3061 gcatcgtgat aatccaaccg ggcaatctgt tcaggaagat cagcagaaaa gacgagcaga
     3121 actccgattc attcctttca cggaattgag ggggcaatgc ggccgcgaac tcacgagcga
     3181 ggcgggacgg ttcaatctgt cgagcgggcc ctgacgctgc tggaggccct gggagattgt
     3241 cggggtgagg tcggtatcgc caatctcagc aagcgagtcg ggcttcacgt gagcacggtc
     3301 catcggatcc tggggacatt gctggagcga ggctacgcac ggcagaatcc cgagacaggc
     3361 cactatggcc tgggcgccaa gacattttct ctggcggaat cctacctcgg gcagatggac
     3421 ctcagccgcc tggtccggcc tgccctggaa cgtctcagcc gggagaccga ggaaacggca
     3481 aatctcgtga tcctggacgg cggggaagcc ctgtacctgg acaaagtcga gagtccccag
     3541 aaccttcgca tcttctcgcg gatcggccat agggtgcccc tacattgctc cgcggtggga
     3601 aaggtgctcc tggcatcacg ggcgcagaag gacgcggatg ctttgctggg ccgcaggccc
     3661 ctggagggcc tcacccggtc caccatcacg tccagggttc agcttcggcg cgagttggag
     3721 aaggtgcggg agcaggggtt cgccctcgac tgcgaggagt gcgaggaggg ggcctgctgc
     3781 atcgctgtcc ctctcaagga tgcgcagggg cgcgtggagg cctcgctctc cgtgtcggga
     3841 ccgtcggtgc gtctcacgcc gaagcgaatg gaagagttga ttcccctcat gcggcaggtg
     3901 gcgcgtgagg tttcgtctca gctagggtgc aataccccgg agggctagag ccgggccggt
     3961 tctcatcctg cgggtcatcc gcgcaaccaa ggagggtcgc atgcccaaga tgccggtcat
     4021 ggaagccgca gtcaagatca tggagagcga gggggtggag gtctgttttg ggattcccgg
     4081 ggcggccatc cttccgttgt acaaggcgct cacgaagtcc agcaagattc ggcatctgac
     4141 cgtgcgacac gaggaggggg cgacgcacgc cgccgacggc tgggcccgga tcaccggcaa
     4201 ggtgggcgtt gccatcggga catctggacc ggccggcaca aacatggtga ctggtctcta
     4261 cacctgcatc ggggactcga tccccattat ctgcatcacg gggcaggcgc tcacagagag
     4321 actccacacg gaggccttcc aggcagtgga catcgccgag atcgccaagc ctgtgaccaa
     4381 gaagagttac tgcgtcaagg aaaccgcgca gattccccag gtgttccgcg aggccttccg
     4441 gatcgcccgg gagggccgac ccggtccggt gctcatcgat ctaccgctga acgtgcagcg
     4501 gggtgaggtg gagtacgatc ccgacatcga cgcacccctg gaggtcttca agccgtctcc
     4561 cgacgcgcga aagattcgcc gcgcgatcga gatgctcctc caggctaaac ggccgatcct
     4621 gatgatgggc ggcggtgtca tgatcgcgcg ggcatcccgg gagttcgtgg ccctggcgga
     4681 gcacctccag atcccggtga tccccacgta catggccgag ggcggaatcg ccttcgacca
     4741 tcctctttac ggcggccggg tgggattgca gaccaacacc cgggccggca acaagctgtt
     4801 cctggaatcc gacctggtcc tgtccatcgg caaccgcttc gccgagcggc acaccgggga
     4861 cctcacggtc tatcgggggg agcgcaagtt catccacatc gacatcgagc ccaccgtgat
     4921 cgggaaggtc cttcccgcgg atctcggcat cgtatccgac gccaagctgg ccgtggaggc
     4981 cctgatgcgg gtggccaaag agatgacccc caagcgctca cccaacgact ggtccaagaa
     5041 ggcggcggac tacaagcgac gcttccgccg gaagatggac tttgaccagg tgccggtgaa
     5101 gccccagcga gtcttcaagg agatcaacga gttcttcgat tccgacacca tcttcgtgac
     5161 ggccatcggc ctgtaccaga tatggtcggg ccagttccag gagatcaaga atcccaacac
     5221 ctacttctgc tgcggccagg ctggtcctct gggatgggag gtgccggcct gcatgggtgt
     5281 gaagctgggg aagcctgatg ctcaagtggt gggtgtggtc ggggattact cattccagtt
     5341 cctgctgggg gacttggcga cggcggtgca gaataacgtg ccctacgtta tcgtcatgct
     5401 gaacaacggg tatctctccc tcatccggca gccctccaag tacctctacc atatgaacta
     5461 cggcgtggat atcggctacg gggacggtta cggccccgac ttcgtcaaga tcatggaggg
     5521 ctttggcggt gccggtaaac gggtcctcaa gcccgaggag atcaagccgg ccctggcctg
     5581 ggccgtccag gagtccaaca atcgcaaggt tcccgtcctg gtcgagatcc gggtggaccg
     5641 cgagacggac gcggccatgg gcgtgtcaat cgacaagatc atcgagcgcg acccggtgat
     5701 cgacctgccc gaggaggccc cggtgaagga accggtctcc gtgcgatagt ctccccatga
     5761 tcgcctgacc ccaccgggca tccgatgctc cgatcggatg cccggttctt tcttctcagc
     5821 ggttccttgt cgggtttcca acttctgggc ctcccaccag gagagatcca gagaaagtct
     5881 tgacttgccg ggagcatctg gatgaagatt cgctccatct tcgccaacct gcagcgatgt
     5941 gcggagggtg cgccggttca ggatcgcgtg gtatagtctc attctttcac aacaggaggc
     6001 cgagcatggc agagcgtatc gggtttctgg gcccagggct catgggcaag ggcatcgtca
     6061 agaatctcct caagaagggc tttccggtca cggtgttcgc ccatcgggtc ggcctgaatc
     6121 tgaacgagct gaagcaggcg ggcgcgtcgg tcacgacctc gctcgccgag gtgggtgaga
     6181 cgagtgaacg ggtcctgctc tgcgtgccgt cctccaaaga ggtcgaggcg gcggtcctcg
     6241 gcacgcccgg cctgttgcag agcatgagga aggggtccgt catcatcgat ctcagcacca
     6301 gctacccggc ctcgacctgc atgctggccg agcgtctggc cgccaagggg atcgccttcc
     6361 tggattcccc catgaccggg acgccggtgc atgccaacgc gggcgagctc aacatcatga
     6421 tcggcgggga caaggcagtg tacgaacaat gcctccccat cttccaggcc atcgccaaga
     6481 acattttcca cgtggggccg agcagccacg ggaacatcgt gaagctgatc aacaatttcc
     6541 tcggccagtt gacgaatgcg ggtcttgccg aagtgctgcc cctggccgtc aagtccggca
     6601 tcgacatcaa tgccctctac gacgtcgtgc gggtgagcgg gggcaacagc cgggtcttcg
     6661 aggggtcggt tccggccatc tgcaagcggg acttcaccgt gaccttcgag atccggttgg
     6721 cgcacaagga catgaactac atgagcagcc tgggccgcga gatgaacgtc cccctgccca
     6781 tggtgaacag cctgttgaac gtcctggacg tcgccaaggc cagcgggctc gcgcgcgaga
     6841 acaccaacgc cctggtcaag atgtgggaaa aggtctccga cgttgtcgtc aaggggtgga
     6901 agtagggctg gggagttgat ccctcagcgg gaggccaaaa cgctggcgga gcgaggcggt
     6961 actattgtag agtagcgcat cacggcggct caggcttacc agcccggaac ctccgccctc
     7021 tgctcccccc tcgaggggaa gggcaagggg tgggtggctg atatctgacc gctgaccgct
     7081 gaatttctgg gagggcaccc gggccctacc cgagcgtgcg tcgttgagca gaggaggcag
     7141 gggacgcagc atgacggtgc ggtgagctcg ccatcggcgg accaccgaca cacgggagga
     7201 tgcgtcatga aacagagctg gtcgttggct gggttgttgg cagttgccct cggattggtc
     7261 ctgggcccgg gtttcgcggc ggacatccgc gcagcggagt tcgacctggt ggaggcgcac
     7321 acgacgcctc ccgatcatcc ctacaccctg gggatggttc actacgctca actggtcaag
     7381 gagcggtcga ggggacgcgt cgccatcca
//
LOCUS       DBNGZC010000251         1520 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1584648, whole genome shotgun sequence.
ACCESSION   DBNGZC010000251 DBNGZC010000000
VERSION     DBNGZC010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1584648"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="AC16G0_04605"
     CDS             <1..469
                     /locus_tag="AC16G0_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0032543 - mitochondrial translation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HIR5098938.1"
                     /translation="APPGAGDLERGLSHIRQMDPHFDEAAFKEWCSDTFFRIQAAWMH
                     RDVERLRPMLGEEMLQVFQTQLDALRAKRQINTLENIAIRSIELTEAWQEQGQDYITV
                     RYLANLLDYTVDEATGKVVEGSDREPVKFEEYWTWVRPVGSNPWRLSAIQQAG"
     gene            475..705
                     /locus_tag="AC16G0_04610"
     CDS             475..705
                     /locus_tag="AC16G0_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098939.1"
                     /translation="MRSRVKGLLARVIRWMLSWASMLAIPLGLGLGLAGHMTLGALLV
                     LGGTAAYFLVDPIMMVLDLFPDGDARRKEKRR"
     gene            745..>1520
                     /locus_tag="AC16G0_04615"
     CDS             745..>1520
                     /locus_tag="AC16G0_04615"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5098940.1"
                     /translation="MRGHWWILGALVLGVLLASAPVRAQELVVYSSVDEENAKKLLDA
                     FSQATGVKVRFTFLSSGPAVARIEAEKGNPQADVWYGAPSENHVVLREKGLSQPYVSP
                     NAKDLAAKFKDPEGYWTSFYMNPLGFASNLSVLKQKGVEAPTSWADLLKPEYKNTIVY
                     LDPRSTGVGQVMVFAANFGMGGDMNNLKPGLDYLTKLHQSGNVLRVLGTTPYAQFVKG
                     EIPIWISYENDGLKAKYIDGLGDAAAVVIPKEASAAAPYA"
BASE COUNT          275 a          462 c          525 g          258 t
ORIGIN      
        1 ggcgccccct ggcgctggag atctggagcg cgggctgtcc cacattcgcc agatggaccc
       61 ccactttgac gaagccgcct tcaaggaatg gtgcagcgat accttcttcc gcatccaggc
      121 ggcatggatg catcgagatg tggaacgact ccgtccgatg cttggcgagg agatgctgca
      181 ggtcttccag acccagttgg acgcgctgcg ggccaagcgg cagatcaata cgctggagaa
      241 catcgccatc cgctccatcg agctgaccga ggcctggcag gagcaggggc aggactacat
      301 caccgtgcgg tatctggcca atctgctgga ttacacggtg gatgaggcaa cgggcaaggt
      361 ggtcgaggga agcgaccgcg aaccggtgaa gttcgaggag tactggacct gggtccggcc
      421 ggtcggctcg aacccctggc gcttgtccgc gatccagcag gcgggctgaa cgccgtgcgg
      481 tctcgcgtca agggcctgtt ggcccgggtg atccggtgga tgctgagctg ggcgtccatg
      541 ctggcgatcc ctctggggct cggcctgggg ctggcgggcc acatgacgct gggagcgctc
      601 ctggtgctgg gggggacggc ggcctatttc ctggtggacc ccatcatgat ggtcctggat
      661 ctcttcccgg acggcgacgc gcgccgcaag gagaagagga gatgaggtct tctggatgac
      721 ccgtgccaca cggtggagga aaacatgaga ggacactggt ggattctggg cgcgctcgtg
      781 ctgggggtcc tgctggcgag cgccccggtc cgggcgcagg agctggtggt ttactcgagc
      841 gtggacgagg agaacgccaa aaaactgctg gatgcgttct cgcaggcgac cggggtcaag
      901 gtccgattca cgttcctctc ctccgggccg gccgtggcgc ggatcgaggc cgagaagggt
      961 aacccccagg cggatgtgtg gtacggcgcc ccgagcgaga accacgtcgt cctgcgcgag
     1021 aagggcctgt cccagcccta cgtctcgccc aacgccaagg acctggccgc caagttcaag
     1081 gaccccgagg gctactggac ctccttctac atgaatcccc tcggcttcgc ctcgaacctg
     1141 agcgtgctga agcagaaggg ggtggaggcg ccgacatcct gggcggatct gctcaagccc
     1201 gagtacaaga atacgatcgt ctacctcgac ccgcgctcga ccggcgtggg acaggtgatg
     1261 gtcttcgccg ccaacttcgg catgggcggc gacatgaaca acctgaagcc ggggctcgac
     1321 tatctcacca agctgcacca gtccggcaac gttctgcgcg tgctcggcac cacgccgtat
     1381 gcgcagttcg tcaaggggga gatcccgatc tggatttcct acgagaacga cgggctcaag
     1441 gccaagtaca tcgacggtct cggcgatgcg gccgcggtgg tgattccgaa ggaagcctcc
     1501 gccgccgcgc cgtacgcgat
//
LOCUS       DBNGZC010000252         5511 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1390424, whole genome shotgun sequence.
ACCESSION   DBNGZC010000252 DBNGZC010000000
VERSION     DBNGZC010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5511
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1390424"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="AC16G0_04620"
     CDS             complement(<1..200)
                     /locus_tag="AC16G0_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013395464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5098941.1"
                     /translation="MGTILTIEDVRKRYAEVEALAGISLQVQAGEFFTLLGPSGCGKT
                     TLLRCVAGFLRPDGGRIMCDGVR"
     gene            complement(326..1963)
                     /locus_tag="AC16G0_04625"
     CDS             complement(326..1963)
                     /locus_tag="AC16G0_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5098942.1"
                     /translation="MRRDIWTGVTGIAFALLLLLLIIPLGRLLWASVLAEDGGLTLAH
                     YRDFLAYPYYSRTIGHSFLVSSLVTFCALLVAVPLAFLVARVDVPGKAVLKTLAILPL
                     VSPPFIGAYSWILLFGRAGYVTTLLQKFGVTIPTVYGMHGIVLALSLNLYPFVLLMVA
                     GALQALDQSLEEAAQGLGSSPWRVFWTITVPVTMPSVLGGALLVFLTAFADFGAPMII
                     GEGYQVLPTIVYSLFVDEMGGNPAKASTAATLLVLCTTAILLAQYWAVGRRRYAMTQL
                     RPIPPVRPRPSLRWLVTAYAYLVVALSLVPAAVVAVTSFFESRGPVLYPNFSLGNYRE
                     VFFNVPRAIANSFVLSSASTILDVLLGTLIAYLVVRRRSATTTALDSCVMVPYAIPGT
                     VIGVAFIVAFNQPPVVLTGTFLILLLAYFIRRLPYSVRAGSAILHQIDPSVEEASISL
                     GVSPLKSFAKVTARLMLPGIVSGGILTWVQTITEISATIFLYFGAWSTITVVIYRQVS
                     SSNFGSAAAASTILLVAVFIPVLLVNWSMGERAVTAV"
     gene            complement(2138..3157)
                     /locus_tag="AC16G0_04630"
     CDS             complement(2138..3157)
                     /locus_tag="AC16G0_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5098943.1"
                     /translation="MRRKWCWSGSVVGGLALALLVAGSAAGAADTEGQVVVYTAAQST
                     IVQAVGPMFEKKTGIKVQFVEAGTGEVIKRAQAEKANPLGDVFWALAAEPLVANADLL
                     EPYTVTEDAKINPIYKKGMAEGRVQPNNVTPMVIVYNKKLVSDAEAPKTWKALADPKW
                     KDKIAYAAADKSGSSYTILATLLTVFGDNDAGWKVVEDIMKNAKILPSSSRVPKGVAD
                     GEYHAGLTYEDAAMRQVAGGAPVGVIYPQDGTSVPPDGNALIKGAKHPKAAKAFLDYV
                     VSKEVMDLLAEKFSLRSARTDVNPPKGFPHLGEIKAVAYDMEWAGKNYAPLVKKWQDI
                     IVRVK"
     gene            complement(3444..4442)
                     /locus_tag="AC16G0_04635"
     CDS             complement(3444..4442)
                     /locus_tag="AC16G0_04635"
                     /inference="COORDINATES: protein motif:HMM:NF025839.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4432 family protein"
                     /protein_id="HIR5098944.1"
                     /translation="MGAARIDPDWRYHDLQAVILENARLRLSLLPEVGAKILDLVFKP
                     ADRNFLWRNPRLEPRRPVFGQNFDDWWAGGWDEVFPTCDVSRYGGETYPYLGELWSLP
                     WSWQVEQEGQDEVCLHLSRTTIIAPARMERWIRLSSEEPVVRFRHRLTNIGTQPMDFV
                     WGLHPCFHVQAGYRIDAPATQGVVGHTATAPFGPVGTMYPWPNAPVAPGGSHDIRIVP
                     PASQGWCEGHYATGLTQGWVALTDPKEEVGIGLRFPQEVFPVLWCWMVYGGWRGHYHV
                     ALEPWTSWPHQLDQAVAAGRHRTLAPGESLECETMAVAYTGLRGVSAIGWDGHVRG"
     gene            4643..>5511
                     /locus_tag="AC16G0_04640"
     CDS             4643..>5511
                     /locus_tag="AC16G0_04640"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR5098945.1"
                     /translation="MPKPAGTPITLKHIADHTGLSIMTVSRVVRDRPDVSAASRRKVL
                     TAVRRLGYIPNPAAQLLASRTVPGTPTDRIIGTIFSREVVTSHSYFAGIIQGIADEAR
                     RFDCHLLFGYGLGDPGGPQDYPKMIREMMTRWIILVGKVPPRFVRQLRGNGFSLVLVD
                     MWPPSRDMDAVVCEDALGAYAATRHLIKLGHRRIALIRGPARHPFSQALTSGYRRALH
                     ESGIPWREDLVLEAPLKPQGGYDAAEVLLKRSGHPTAIFTNDDLAIGALRAIHEQGLR
                     VPEDIALVGFDDI"
BASE COUNT          998 a         1867 c         1725 g          921 t
ORIGIN      
        1 cgcactccgt cgcacatgat gcggccgcca tccggccgga ggaatccggc cacgcagcgg
       61 aggagtgtcg tcttcccgca gccgctggga cccaggaggg tgaagaactc gcctgcctgc
      121 acctggagcg agatgccggc cagcgcctcg acctcggcat accgcttccg aacgtcctcg
      181 atggtcaaga tcgtgcccat ggcacctcgg actcgaagag cgttcggcag tacggctgtt
      241 cggcggttcg gcgaaccctc gaacgcccga actgtctaac cgccgaacgc gctttgccgc
      301 aatgctcccc tgctcctttt cgccctcaga ccgccgtcac ggcgcgctcc cccatcgacc
      361 agttcacgag caagaccggg atgaagaccg ccaccaggag gatcgtggag gcggcagcgg
      421 cgctgccgaa gttgctgctg ctcacctgcc ggtagatgac gacggtgatc gtggaccagg
      481 cgccgaagta gaggaagatg gtggcgctga tctccgtgat ggtctggacc caggtgagga
      541 tgccccccga cacgatcccc ggaagcatga ggcgcgccgt gactttggca aagctcttga
      601 gcggcgacac ccccaggctg atggaggctt cttccaccga gggatcgatc tgatgcagga
      661 tggcggaccc cgcccgaacg gagtacggaa gacgccggat aaagtaggcc aggagcagga
      721 tcaggaaggt gcccgtcaag acaacgggtg gctggttgaa ggccacgatg aaggcgaccc
      781 cgatgacagt ccccgggatg gcgtagggga ccatgacgca ggaatccagc gccgtggtgg
      841 tcgccgagcg ccggcggacc accaggtagg caatgagcgt ccccaggagg acgtccagga
      901 tggtggatgc gctggagagg acgaagctgt tcgcgatcgc ccgaggaacg ttgaagaaga
      961 cttcccggta attccccagg ctaaagttgg gatagagcac tgggcctcgg ctctcgaaga
     1021 acgaggtcac ggccacgacg gctgccggga ccagggacag ggcaaccacc aggtaggcgt
     1081 aggccgtcac cagccaccgg aggctggggc ggggccggac cggcgggatg gggcgaagct
     1141 gcgtcatggc gtaccgcctc cgccccaccg cccagtactg cgccagcagg atggcggtcg
     1201 tgcacaggac caagagcgtg gccgcggtgc tggccttggc aggatttccc cccatctcgt
     1261 ccacgaacag gctgtacacg atggtcggaa gcacttgata cccctcgccg atgatcatgg
     1321 gcgccccaaa atcggcgaag gccgtcagga agacgagcag cgcgccaccc aggactgacg
     1381 gcatggtcac ggggaccgtg atggtccaga acacccgcca ggggctggac cccagaccct
     1441 gggccgcctc ctccagcgac tgatccaggg cctggagcgc ccccgccacc atcaagagca
     1501 cgaatgggta cagattgagc gagagggcca ggacgatgcc gtgcatgcca tagaccgtcg
     1561 ggatcgtgac gccgaacttc tggagcaggg tcgtgacgta cccggcacgc ccgaagagca
     1621 ggatccagga gtaggccccg atgaagggtg gcgacaccag cggcaggatg gccagggtct
     1681 tcaggaccgc cttccccggc acgtccaccc gggccacgag gaatgccagg gggacggcca
     1741 ccagcaaggc gcaaaacgtc accaggctgc tgaccaggaa gctatgtccg atggtccggg
     1801 agtaataggg atacgccagg aagtcgcgat agtgggccag cgtcaacccg ccgtcctccg
     1861 ccaggacgct ggcccagagc aatcgcccca atggaatgat caggaggaga aggaggaggg
     1921 cgaaggcgat ccccgtcacg cccgtccaga tgtctctacg cacagctatc agccttcagc
     1981 gggctgccag gaacacgaat cgcgttcccc gttcacccct gcccccctgc gcctctgctc
     2041 ctccgcggtt ctgtaccctg tgccccgtgc cccgtgctct gtgccctatg ctctgtgccc
     2101 tgtgctctat gccctgtgct atatgccctg tgctctttca cttcacccgc acgatgatgt
     2161 cctgccactt cttgacgagg ggggcgtagt tcttcccggc ccactccatg tcgtaggcca
     2221 cggccttgat ctcgccgagg tgggggaatc ccttgggcgg attcacgtcc gtccgcgccg
     2281 agcgtaggga gaacttctcg gccagaaggt ccatcacctc tttggagacg acgtagtcca
     2341 ggaacgcctt ggctgccttg ggatgcttgg cccccttgat caaggcattg ccgtcgggcg
     2401 ggacggatgt gccgtcctgc gggtaaatca ctcccaccgg tgcgccgccg gccacttgtc
     2461 gcatggccgc gtcctcatag gtcagacccg cgtggtactc gccgtcggcc acccccttgg
     2521 gcacccggct ggatgacggg aggatcttgg cattcttcat gatgtcctca accaccttcc
     2581 agccggcgtc gttgtcaccg aacaccgtga ggagcgtcgc caggatggtg tacgaggagc
     2641 cggacttgtc ggccgcagca tacgcgatct tgtccttcca cttgggatcc gccagcgcct
     2701 tccaggtctt gggagcctcg gcatcactca ccagcttctt gttgtagacg atgaccatgg
     2761 gcgtcacgtt gttgggctga acccggccct ccgccatgcc tttcttgtag atggggttga
     2821 tcttggcgtc ctcggtcacc gtgtacggct ccaagaggtc tgcgttcgcc accaagggct
     2881 cggctgccaa ggcccagaag acatctccca gggggttggc cttctcggct tgcgcgcgct
     2941 tgatgacttc tccggttccc gcctcgacga attgcacttt gattccggtc ttcttttcga
     3001 acatcggccc gacggcttgc acgatcgtgc tctgcgccgc cgtgtagacc accacctgcc
     3061 cctcggtgtc ggctgcccca gcggcggacc ccgccaccag gagtgccagg gccaacccgc
     3121 ccaccacgct cccgctccaa caccactttc gtcgcatggc ctgtcctccc cctgtagggc
     3181 ggcaacctcc tcctttatat atagggaggt cgctcggccc aggttaactc tgatccgaaa
     3241 ttcggcatct cgccaagatc acccaacccc aggccgggta cccggatcgc caagacccac
     3301 gagcacaatc cgggcacagc aggcaagaga tccaccgccc ctggaaggag tcatccgata
     3361 tgggtgattc cgtgattaaa tctggccttt gtttgcgccc ttggcgtcat tgcgagagtc
     3421 ggatttcttc ggactgggct taattatccc cgaacatgac catcccagcc gatcgcgctc
     3481 acacctcgga gaccggtgta ggccacggcc atggtctcac actccaggga ctcccccggc
     3541 gccagggtcc ggtgccggcc ggccgccacc gcctggtcga gttgatgcgg ccagcttgtc
     3601 cagggctcca gcgcgacgtg gtaatgtccc cgccatcccc catacaccat ccagcaccag
     3661 aggaccggaa acacttcctg cgggaagcgg aggccgatcc ccacctcctc tttcgggtcc
     3721 gtgagggcca cccatccctg agtgaggccg gtagcatagt gcccctcgca ccagccctgg
     3781 ctcgccggtg gaacaatgcg gatgtcgtgc gagcctccgg gcgccacggg cgcattcggc
     3841 caggggtaca tggtccccac cggcccgaag ggcgccgtgg ccgtgtgacc gaccacgccc
     3901 tgagtcgccg gagcatcgat tcggtatccc gcctggacgt gaaagcacgg atggaggccc
     3961 cacacgaagt ccatgggctg cgtcccgatg ttcgtcagcc ggtggcggaa acggaccacc
     4021 ggctcctctg agctgaggcg gatccacctc tccatccggg cgggcgcgat gatcgtcgtg
     4081 cgcgacaggt ggaggcagac ctcgtcctgg ccttcctgct ccacctgcca cgaccaggga
     4141 agcgaccaga gttctcccag gtagggatag gtctcacccc cgtagcggct cacgtcgcag
     4201 gtgggaaaga cctcgtccca gcccccggcc caccaatcgt cgaagttctg gccgaagacg
     4261 gggcgtcgag gctccaggcg agggtttcgc cagagaaagt tccggtcggc cggcttgaag
     4321 accaggtcga gaatcttggc cccgacctcg ggaaggaggc tgaggcgcag gcgggcgttc
     4381 tccaggatca cggcctgcaa atcgtggtag cgccagtcgg gatcgatccg agcggccccc
     4441 atgctctcct ccgccaatgc ccccctgcct tcgggactaa atcacacgtt tgaacagaaa
     4501 tcaacacgaa atcacatctg gcctcttggt gcgtcctccg gggtcgcggg ccgaggaaac
     4561 atgtcctgga aaaagcgctt gacgctcggg tctacctggg gtagctatat taccgttaac
     4621 gctaacaggt catcggaaag acatgcccaa gcctgccggg acgcccatca cgctcaagca
     4681 catcgcggac cacaccgggc tgtcgatcat gacggtctcg cgggtggtgc gggaccgccc
     4741 ggatgtcagc gcagccagcc gtcgcaaggt cctgaccgcc gtccggcggc tgggctacat
     4801 ccccaatccc gccgcccaac tcctggccag ccgcaccgtc cccgggacgc caacggaccg
     4861 aatcatcggg accatcttca gtcgcgaggt ggtcacctcc cacagctact ttgccgggat
     4921 catccaggga atcgccgacg aggcccgccg cttcgactgc cacctgctct tcggctacgg
     4981 cctgggggac ccaggcgggc cgcaggacta ccccaagatg atccgcgaga tgatgacccg
     5041 ttggatcatc ctggtgggaa aggtcccccc gcgattcgtc cgccagttgc ggggcaacgg
     5101 cttctccctg gtcctggtgg acatgtggcc gcccagccga gacatggacg ccgtggtctg
     5161 cgaggacgcc ctgggggcat acgccgccac gcgccacctg atcaagctcg gccaccggcg
     5221 catcgccctc atccggggtc ccgcgcgcca tcccttctcc caggccctga ccagcggcta
     5281 ccggcgcgcc ctgcatgagt ccgggatccc ctggcgggag gatctcgtcc tggaggcgcc
     5341 gctcaaaccg cagggaggct acgatgcggc ggaggtgctc ctcaagcggt cggggcatcc
     5401 cacggccatc ttcaccaacg acgacctggc aatcggggcc ctgcgggcga tccatgagca
     5461 ggggctgcgc gtccccgagg acatcgccct ggtgggcttc gacgacatcg a
//
LOCUS       DBNGZC010000253        11263 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_411766, whole genome shotgun sequence.
ACCESSION   DBNGZC010000253 DBNGZC010000000
VERSION     DBNGZC010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 11263)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11263)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11263
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_411766"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="AC16G0_04645"
     CDS             <1..870
                     /locus_tag="AC16G0_04645"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR5098946.1"
                     /translation="IRTQRPARDLLDNGRYLKVGGMANLQTKRGCPFRCAYCTYPQIN
                     GPTLRLRPPAAVVDELASMVDGSELNQVFIVDDVFNVPQDHAMAICEEIVARGLRVGW
                     TCFASPLGMTLELASAMKRAGCQGVEFGTDTASPFMLQSMGKPFSQEEIRTASRACRA
                     AGLPDAHYLIFGGPGETPETLAETLAVFDELKPRAVLAFLGIRVYPNTILHRHAISNG
                     VIAEEDDLLYPRFYISRGVGADTLKASVGSHAEARPNWVVPGLGIRSDPALLAALQRI
                     GRRGPLWNLLESP"
     gene            885..1454
                     /locus_tag="AC16G0_04650"
     CDS             885..1454
                     /locus_tag="AC16G0_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098947.1"
                     /translation="MRRTLGILMVLWAVHLLVPHAGNAEEDAYYARCNLKVQDNNEIT
                     WVNWQAAPTFIPVGTKLRVTRTGDKASLVNIETKTAYTLDIGADGQAFLEKFVSLGPI
                     DIQRFPEDVQTNIRNAVAKIGMTKEQVYIAMGPPTNLGRSRTNAMTYENIMAGDLWVY
                     ARRRFGKNIGVAFDPASGMVNRTEGIWGK"
     gene            1594..2322
                     /locus_tag="AC16G0_04655"
     CDS             1594..2322
                     /locus_tag="AC16G0_04655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5098948.1"
                     /translation="MGRVVLVTGGSRGIGKSIALAFASPGTVVGINYRSDEQAAMEVL
                     TELRGRGAEGMALRADVTDLVEVRGMIDTLVSRWNGIDVLINNAGGHRDGLLALMDPA
                     DWDAIIDLNLKGVFHCCKTAIRPMMAQRTGAIVNVSSLSGIMGLPGQTNYAAAKGGVN
                     AFTRALALEVARFGIRVNAVAPGLVETEITAQIPTAQRERLLSGVPMGRMAKPDEIAA
                     VVEFLASEKASYITGQVIQITGGI"
     gene            2382..2627
                     /locus_tag="AC16G0_04660"
     CDS             2382..2627
                     /locus_tag="AC16G0_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR5098949.1"
                     /translation="MERVRGLLSTRLKLGKTAEAIGDETPLFGPEGLGLDSIDALELV
                     LGLEKEFGVVIEDRPTAVKVLASIKTIAEFIQAQGKG"
     gene            2638..3063
                     /gene="fabZ"
                     /locus_tag="AC16G0_04665"
     CDS             2638..3063
                     /gene="fabZ"
                     /locus_tag="AC16G0_04665"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIR5098950.1"
                     /translation="MLGDPLARLPHGIPFRFVDRVLELIPGERVVTLKNVTANDPVIQ
                     GHFPDNPILPGVLLLEAMAQSSGLLLPVGSSALLAQIRDARFRRTVVPGDQVRIEAAR
                     LTGLGDLHRFEVKAYVDSQPVAEAEIVLAVSNAVGKDGA"
     gene            3329..3823
                     /locus_tag="AC16G0_04670"
     CDS             3329..3823
                     /locus_tag="AC16G0_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098951.1"
                     /translation="MKPSTSQVVPTKDKALVRFMRPSGIGFAVNFNLLDGEKVIGNSV
                     AKSQFDYLADPGHHLFISISENKVFLEADLAPGKVYYVITRIYPGWVRPRVAFEAVTR
                     GSEYWDAVLQYEKELQRLEPDEATIRVWQDAHREEIRALFSSYEAEFKTKYNWPKLSP
                     DDGR"
     gene            3883..4746
                     /locus_tag="AC16G0_04675"
     CDS             3883..4746
                     /locus_tag="AC16G0_04675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5098952.1"
                     /translation="MNAVPATETVFCNLCASDDAGPIAHQNGYRIVRCRTCGLVYVNP
                     RPAADDLRSLYADYHFRKGEDEASWDRLMRWVFREAAEVLVSCRDGSGPGRLLDVGCG
                     FGAFIAGMRERGWEAEGLDPSPTVVSAATRNGRPVRLGTLEALKGDRGVYDAVTMFYV
                     LEHLPDPMGALKKAWGLLAPGGCILIRVPHTTPIVRLLAPLGLDGALYDPPFHLYDFS
                     PDVVREMLRRTGFVDIRTFPGRPTLPSRLGPRIASALFGALARGVHALTGGLVLLPGV
                     SKTTMARKPSE"
     gene            4770..7256
                     /locus_tag="AC16G0_04680"
     CDS             4770..7256
                     /locus_tag="AC16G0_04680"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5098953.1"
                     /translation="MARTVDAKPRGYSIACFILAALAAIVVVRIPVSTDILDVLPEKN
                     PAIAAFTEFLRDFGILDGLVIVVESEDRSIDSLIGTVQTLGERLAVSPYVASVDYNLL
                     RSDSQFIAEHFPVYLGASGITRLAQRLTPEGIRIQIRKDREALLSPLAPPFEAEMIRL
                     DPLNLREFVQESLVRRLPTKALDVSTGYYLDRSHRLALLMVRPKGSVRDMSFVRGLRG
                     EVGRIAAEAIRQAENPQGMKIGLAGGYARAAEALSVIWQDMVVSFAVSLLLILLVLYF
                     AFRPSLRVLGIFVVTMFASLAWTLLLAYFLFGTLNIITSIVAAMLIGLFVDYMIQMYR
                     RFEECYRREGSPSRALERTLTGTGKAIVSSALTTAVSFFSIVITSFRGLHELGVVAGF
                     GIIFCLLATLVLMASLLSWLAKSCPRYLPAGRPVDFGVNWAARLVDRRGRTLVLIFST
                     LLALGFAGTMRLRFDTSVESMGLRQSAVQSVEQKIEQVLGRRGEPLFVVARAPGEDRL
                     ASDFDELERQGERWRAAGRVGSFSSLGMLLPPPYLQREALTRLSAEGLTGKISGPDLA
                     DTVRKEMDRQGMVADASLEGYATRIARALAVREVVSPRQFSQAQNPRAIYFSNPERTA
                     IAAHLTPPGPRWDRAGVLALAEDVRRLGTDFRLVGPAIFLDEIKTTILWEAGAAVMLT
                     FAANLLIVWYHFRRWGRVWLVMLPVTAGTILTLGGMGILGMQFNFFNVAGIALIFGFG
                     VDYGIYLMQADTEIGSGKALSAVRSTGGNIVLCAVTTMASSGSLITTHYRGLASVGAV
                     LCLGALFCLVSTLLLLPALLASSRTVSSQP"
     gene            7253..8698
                     /locus_tag="AC16G0_04685"
     CDS             7253..8698
                     /locus_tag="AC16G0_04685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR5098954.1"
                     /translation="MKWDAVIIGSGIGGLACAGMLATRGRRVVVLEQAVAPGGYLTSF
                     QREGFTFDSAVDCIAGLDPQGLLTWLLRSLGVEDGLTPIRLDPIRVSRFPGMAVQVDA
                     SLPAYIERLSRLFPSERDGIASFFRRAGEIYADVEAMMEIMRDGEGKDDVFPGAMMRY
                     HDLTHADLLRLDIRDVRLAAILSDRCPFLGCSPVRVSANRMASLMMSYFRSGAFRPMG
                     GHQRLPELLTQGIRRMGGEVHCGRPAKRILLEGGRCARVLTEDGGEFPAQHVVSNSDF
                     AETFGRLIGGDVGRVVLGEASGRPLSPSFFILYAGVRTAAPSCAASSIGSFQSFDLGD
                     LLDRYVPFSKADALGITIPTVEDPSLAPPDHEVCIVHELIPHWDTRDWRLEEKICTQK
                     VLGKAERVFPGLTRRLAYCQAATPATLERYTRNRGGAAYGWDQIPHLPKVRHGIGNLH
                     LAGHWAETGGGVLAAAYSGMRIAVRILRATS"
     gene            8695..9741
                     /locus_tag="AC16G0_04690"
     CDS             8695..9741
                     /locus_tag="AC16G0_04690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5098955.1"
                     /translation="MNGRASVCGIIPAYNARETIAGVVQGVLRHLETVIVADDGSTDG
                     TAEVARRAGAGVIVLGENRGKGDALRILFAEAQRRGFSAAMAVDADGQHDVDDIPSFL
                     RAHRDSPDAILTGSRMGARDHIPRHRHNSMLVARYFISLAANQFIEDTQCGFRLYPFS
                     VIGSLSLLKGRYVTETEILIKAGDSGRQIRCVPIKAHYPPGQLTHFRSVSDIAAISLY
                     VISYLMVKWGIEGVRPGVSNTYRGGGTGRDVFFLSPFLDWIFEVLTFSFALPLSVAYG
                     AWYYVARFFSVPALQSLARNSVPVGRLVCSVLLLPVLLILSILDLLGHRMRIPSDLTT
                     PFVRRWYSIPYHQE"
     gene            complement(9841..>11263)
                     /locus_tag="AC16G0_04695"
     CDS             complement(9841..>11263)
                     /locus_tag="AC16G0_04695"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR5098956.1"
                     /translation="FIHAEDSGNATARFADALRHPGQSLRIELRVRHTDGSWRWIEFG
                     IRSWLDDPSVQGVVGNWRDISVTKQAEEALRAAQAEMDSGFRDRTDGLTRANEELQAV
                     LGQRQQAEEAVRESQAQLAAIIGSAREAIISIDETQRIVLFNAAAERMFRVSAAEVMG
                     QPVQRFIPEQRRATHLAGLRDFGRTNVERWSIGLLGGATALRADGEEFPMEAAISQVE
                     FGGRKLYTAILRDVTDRKRAEEDLQASREQVRALTRRLLLVREDERLRVAQELQERLG
                     QALTALKLDLAWLATKLPADQAPLPGKLKAMLAVADSAIKSARRLATELRPGILDDLG
                     IVDAIEWQAQEFQAHTSIRCEFASDRPDVRLDPEASTALFRICQECLTNVARHARASR
                     VHIRLGEETGHFVLMVHDDGQGITEQALTSRTSLGLLIMREQACLFGGQVFIVGRPRE
                     GTTVTVRIPMKQIIAENASEGRS"
BASE COUNT         1924 a         3430 c         3644 g         2265 t
ORIGIN      
        1 atccgaaccc agcgcccggc tcgcgacctg ttggataacg gtcggtatct caaggtgggc
       61 ggtatggcga acctgcagac gaagcgcggc tgcccattcc ggtgcgccta ttgcacctac
      121 ccccagatca acggtccgac tctgcgcctc cggcctcccg cggcggtggt ggatgagctg
      181 gcatcgatgg tggatggatc cgaactcaat caggtgttca tcgtcgatga cgtattcaat
      241 gtgccccagg accacgccat ggccatctgt gaagagatcg tggcgcgggg gctacgggtg
      301 ggatggacct gcttcgccag ccccctgggg atgaccttgg agctggcctc cgccatgaag
      361 cgcgcgggtt gccagggtgt cgagttcgga accgatacgg cctccccgtt catgctgcaa
      421 tccatgggga agccgttttc ccaggaggaa atccggactg cctcccgtgc gtgccgagcg
      481 gccggattgc cagatgccca ttacctcatc ttcggcgggc cgggtgaaac gccggagacc
      541 ctggcggaga ccttggccgt tttcgacgaa ctcaaaccgc gggccgtcct ggcgttcctc
      601 ggtatacggg tctatcccaa cacgatcctc cacaggcacg ccatctcgaa cggcgtgata
      661 gccgaagagg atgacctgct ctacccccgg ttctatatct cccggggggt cggggcggat
      721 acgctgaagg cttcggtggg ttcccatgcc gaagctcgcc cgaactgggt cgtgccgggc
      781 ctggggatcc gttcggatcc cgcgctcctg gcggcgttgc agcggatcgg gcgccggggc
      841 cctctctgga atctgctgga gagtccgtag gccggagggt gacaatgaga agaaccctgg
      901 ggatcctgat ggtcctgtgg gcggtccacc tcttggttcc tcatgctggg aacgcagagg
      961 aggatgccta ctatgcccgg tgcaatctga aggtccagga taacaatgaa atcacctggg
     1021 tgaactggca ggcggctccc acgttcattc cggtgggcac gaagctccgg gtgacaagga
     1081 ccggcgataa agcgtcattg gtgaacatcg agaccaagac agcctatacc ctggacatag
     1141 gggccgatgg gcaggccttc ctcgagaagt tcgtcagcct gggtcccata gacatccagc
     1201 ggtttccgga ggacgtccaa acaaacattc ggaatgccgt cgcgaagatc gggatgacca
     1261 aggagcaggt gtacattgcc atgggcccac ctacgaacct ggggaggtcc cgcacgaacg
     1321 ccatgacgta cgagaatatc atggccggag acctgtgggt gtatgctcgt cggcgttttg
     1381 gaaagaatat tggggtcgcc ttcgatcccg cctctggcat ggtgaatagg acggagggta
     1441 tctgggggaa ataggaggac tctctggggg ggccgatggt tcacaagagt caggtgaata
     1501 ctgcggtcct gtccactact ccgcccgggt cccataggga gcgggtagcc tttccgggag
     1561 ggtctgcgca tcgggacatg gatcgagact gatatggggc gggtcgtcct tgtgacgggc
     1621 ggaagccggg gcatcggaaa gagcatcgca ctggctttcg cctctccagg aactgtcgtc
     1681 ggcattaact accgaagtga cgagcaagcc gccatggagg tgctgactga actcaggggt
     1741 cgaggtgcgg agggtatggc ccttcgggcc gatgtgaccg acctggtgga ggtgcggggt
     1801 atgatcgaca ccctggtctc ccgctggaac gggattgacg tgctgatcaa caatgcgggt
     1861 gggcatcggg atggcctgtt ggccctgatg gacccggcgg actgggatgc catcatcgat
     1921 ctcaacctga agggggtgtt ccattgctgt aagaccgcga tacgccctat gatggctcaa
     1981 cgcacgggcg ccatcgtgaa cgtgtcctcc ttgagcggga tcatgggact gccggggcag
     2041 acgaattatg cggccgccaa ggggggggtg aatgccttca cgagggcgct ggccctggaa
     2101 gtggcgcggt tcggcatccg tgtgaatgcg gttgcgcccg gcctggtcga aaccgagata
     2161 acggcacaga ttccaaccgc gcagcgggag cgactgcttt ccggcgttcc gatgggtcga
     2221 atggctaagc cggatgagat cgctgcggtc gtcgagttcc tggcttcgga gaaggcatcg
     2281 tacataacgg gacaggtcat ccagatcacc ggaggaatct gaaacgaccg gggtacatcc
     2341 ccggtgccaa cctcaaagga tttctgctaa tgaatgagat cgtggaacgc gtcagaggat
     2401 tgctctctac caggctgaag ctgggaaaga cggcagaagc gatcggtgac gagacaccac
     2461 tcttcggccc ggaaggtttg gggttagatt ccatcgacgc gctggagctt gtgctcgggc
     2521 tggagaaaga attcggggtc gtcatcgagg atcgccctac ggcggtcaaa gtcttggcgt
     2581 cgatcaagac gatcgcggag ttcatccaag ctcaaggaaa gggctgagcc cttggacatg
     2641 ctcggagatc ccctggcgcg cctcccgcat gggattccgt tccggttcgt tgaccgggtc
     2701 ctggagctga tcccgggaga gagagttgtc acgctgaaga atgtgacggc gaacgatccc
     2761 gtcatccaag gacacttccc cgacaatcct attctgcctg gggtgcttct tttggaggcg
     2821 atggcccagt cctcgggatt gctcctgccg gtgggttcgt ccgctcttct ggctcagatc
     2881 agagacgctc gctttcgccg gaccgttgtt cctggcgacc aggtccgcat cgaggccgca
     2941 cgcctcaccg ggttggggga tctccatcgg tttgaggtca aggcttacgt cgatagccaa
     3001 ccagtcgcgg aggcggagat cgtgctggcg gtttccaacg cagtgggcaa ggatggggca
     3061 taagtgggat caatgtcatt gttccggaaa tgaccgagga gatctacgtt caggctgatt
     3121 agggatgaga aaccggttcg aagggggggt gcagaatcag ctcgttagac gttttcccgg
     3181 taatcaacga atggctcctg atcgacgatg gaggtggcag atgaccaaga tgatgatatg
     3241 tccgagatgg gtacgggcga ccgcctgctg cctgagtcta tttctattcc ctctggtctt
     3301 atcttcctgt gccggatctt ccaattacat gaagccatct acgtcccagg ttgtcccgac
     3361 gaaagacaag gccctcgtgc ggttcatgcg accctccgga atagggttcg cggtgaactt
     3421 caacttgttg gacggagaga aggtcattgg caatagcgtg gccaagtcgc agttcgacta
     3481 tctggcggat ccgggccatc atctctttat ttcaatctcg gagaacaagg tcttcttgga
     3541 agctgatctc gcccccggaa aagtgtatta cgtcatcacg cgcatatatc cggggtgggt
     3601 gcgtccaagg gttgcgtttg aagcagtcac gaggggatcg gagtactggg atgcggtcct
     3661 ccaatacgaa aaggaattgc agcgattgga gcccgatgag gccaccatac gggtctggca
     3721 ggatgcccac cgggaggaga tacgagccct gttctcctcc tacgaagctg aattcaagac
     3781 aaagtacaac tggcccaagc tgtccccgga tgacggccga taagaggcat gtcgaggggg
     3841 tctgaacgtg atgggggtgg tgagacccaa ggaagcggcg aattgaatgc agttccggca
     3901 accgagacgg tcttctgcaa tctgtgtgct tccgacgacg cgggaccgat tgcccatcag
     3961 aacgggtacc gcatcgtccg gtgccgcacg tgcggtctgg tgtatgtcaa tcctaggccg
     4021 gccgcagacg atctccgttc tctgtatgcg gattaccatt tccggaaggg agaagacgag
     4081 gcgtcgtggg atcggctgat gcggtgggtt ttccgcgagg ctgcagaggt gctcgtctcg
     4141 tgccgggatg gctccggtcc cggtcgtctc ctggatgtgg ggtgtgggtt cggcgcgttc
     4201 attgccggca tgcgggagcg gggatgggag gcggagggat tggacccctc gccgacggtc
     4261 gtctccgctg cgacacgcaa tggtcgtccg gtgcggctgg gaacactgga ggcattgaag
     4321 ggtgaccgtg gggtctacga cgcggtcacg atgttctacg tgttggagca ccttcccgat
     4381 cccatggggg ccctgaagaa agcctggggt ctgcttgcac ctggtggctg cattctgatc
     4441 cgggttcctc acacgacccc catcgtccgc ctccttgccc ccctcggcct ggatggggca
     4501 ctgtacgacc caccgtttca cttgtatgat ttctccccgg acgtcgtccg agagatgctc
     4561 cggcggacgg gcttcgtgga tatccggaca tttcccggaa ggccgaccct cccgtcccgc
     4621 ctggggccgc gaatcgcgtc cgcactattc ggggcgctgg ccagaggggt tcatgccctg
     4681 actggcgggc tggtgctcct gccgggggtg agcaagacta ccatggctcg gaaaccgtcg
     4741 gaataatgtt ggatcggtcc ctccgctggt tggcgcgaac ggtcgacgcc aaaccgcgcg
     4801 gctattctat cgcctgcttc atcctggccg ccttggcagc catcgtcgtc gtgcggattc
     4861 cggtctcgac ggacatcctt gacgtcctgc cggagaagaa tcccgccatt gccgccttca
     4921 cggaattcct gcgggatttc gggatcttgg acgggctggt catcgtggtg gaatccgagg
     4981 atcggtccat cgactccctg atcggcaccg tacagacact cggcgagcga ctggccgtct
     5041 ctccctacgt tgcctcggtg gactacaacc tgcttcgctc ggactcccag tttatcgccg
     5101 agcatttccc cgtgtatctg ggggctagcg ggatcacccg actggcgcag cgtctcacac
     5161 ccgaggggat tcgcatccag atccggaagg accgagaggc cctcctgtcc cctttggccc
     5221 cacccttcga ggccgagatg atcaggctgg acccgttgaa tctccgggaa ttcgtccagg
     5281 agagcctggt caggcgcctg ccgacgaaag ccctcgacgt ttccacgggg tactatctgg
     5341 atcgatcgca tcgcttggcc ctcctcatgg tgcgccccaa gggctctgtc cgggatatgt
     5401 catttgtgcg cggcttgcgc ggggaggtgg gccggattgc ggccgaggcg atccggcagg
     5461 ctgagaaccc ccaggggatg aagatcggtc tggcgggagg ctacgcccgc gccgccgaag
     5521 cgctgtccgt catctggcaa gacatggtcg tctcctttgc cgtgtcgctc ctcctgatat
     5581 tgctcgtcct ctattttgcc ttccgccctt ccctccgggt gctcggtata ttcgtggtta
     5641 cgatgttcgc ctccctggcc tggacactgc tgctggccta cttcctgttt ggaacgctca
     5701 acatcatcac cagtatcgtc gccgcgatgc tgatcggtct gttcgtggac tacatgatcc
     5761 agatgtacag gcggttcgag gagtgctatc gccgggaggg aagcccctca cgggccctgg
     5821 agaggaccct cacgggcacg ggcaaggcga ttgtcagcag cgccctcacc accgccgtct
     5881 cgttcttttc catcgtcatc accagctttc gcggtctgca tgaactgggc gtcgtggccg
     5941 gcttcgggat catcttctgc ctgcttgcca ccctggtgct catggcatcg ctcctctctt
     6001 ggctggccaa gagctgtccg cgataccttc cggccggccg gccggtggac ttcggggtga
     6061 actgggcggc gaggctcgtc gaccggaggg ggaggacgct cgtcctcatc ttctccacgc
     6121 tattggcgct cggctttgcg ggcaccatgc gcctccggtt cgacacgagt gtcgaatcca
     6181 tggggctccg gcagagcgcg gtgcagtcgg tggagcagaa aatcgagcag gtgctcggac
     6241 gacgggggga gccgctcttc gtcgtcgccc gggcgcccgg agaagaccgg cttgccagtg
     6301 acttcgatga actggagaga cagggggagc gctggcgggc ggccggcagg gtagggagct
     6361 tctcttccct ggggatgctc ctacctcccc cgtacctgca gcgggaagcc ctgacccgac
     6421 tctccgccga gggcctcact ggaaaaatta gcggacccga cctcgccgac accgttcgga
     6481 aggagatgga ccgtcagggg atggtggccg atgcttccct cgagggttac gcgacccgga
     6541 tagcccgggc tctggccgtg cgcgaggtcg tttcgcctcg gcagttctcc caggcgcaga
     6601 atccgagggc gatatacttc tctaatcccg agcgaacggc tattgccgcg cacctgacgc
     6661 cgccgggacc acgctgggat cgtgctggcg tcttggccct ggcggaggac gttcgtcgtc
     6721 tggggacgga tttccgcctc gtcgggcctg ccatcttcct ggatgagatc aagacgacga
     6781 ttctctggga ggcaggggct gccgtgatgc tcaccttcgc cgccaacctt ctcattgtct
     6841 ggtaccattt cagacgatgg ggccgcgtct ggctggtcat gctcccggtg accgccggaa
     6901 ccatcctcac gctggggggc atggggatcc tcggcatgca gttcaatttc ttcaacgtgg
     6961 cgggaatagc cctcatcttt ggattcgggg tagactacgg aatctacctg atgcaggcgg
     7021 acaccgagat tgggtctggg aaggccttga gcgccgtgcg gtccactggg ggaaacatcg
     7081 tgctctgcgc tgtcacgacc atggcgagca gcggaagcct gatcaccacc cactatcgcg
     7141 gcctcgcctc cgtgggggcc gtcctttgtc ttggcgccct attttgcctc gtctccacac
     7201 ttctcctcct accggccctc ttggcgtctt ccaggactgt gagcagccaa ccgtgaaatg
     7261 ggatgcggtg atcatcggca gcggcatcgg cggtctcgcc tgtgccggca tgctggcgac
     7321 aaggggcagg agggtggtgg tgctggaaca ggccgtcgct cccggcggtt atctgacttc
     7381 ctttcagcga gagggattca ccttcgattc ggctgtggac tgcatagccg gcctcgaccc
     7441 ccaggggctg ctcacctggc tgctccgatc cttgggggtg gaagatggcc tcaccccgat
     7501 ccgcctcgat cccattcggg tcagccggtt tccgggtatg gccgtccagg tggatgccag
     7561 cctcccggcg tacatcgagc gactctcccg gctctttccg tccgagcgag acggcatcgc
     7621 gagcttcttc cgacgggccg gagagatcta cgccgatgtc gaggcgatga tggagatcat
     7681 gcgggacgga gagggaaagg atgatgtctt tcctggcgcc atgatgcgct accacgacct
     7741 gacccacgcg gatctcctcc gcttggatat tcgtgacgtg cggctggcgg ccatcctgtc
     7801 ggaccgatgc ccgttcctcg gctgctcgcc ggtgcgggtg tctgccaacc gcatggcctc
     7861 gctgatgatg agctacttcc gttccggagc ctttcggccg atgggcgggc accaacgcct
     7921 cccggagctc ctcacgcagg ggatcagacg gatgggcggc gaggtgcatt gcggccgtcc
     7981 ggcgaagcgg atcctcttgg agggcggtcg gtgcgctcgg gtcctcaccg aggacggtgg
     8041 ggaatttccg gcgcagcacg tggtctccaa ctcggacttc gccgagacct tcggccggtt
     8101 gatcgggggc gatgtgggaa gggtggtcct cggagaggct tccggtcggc ccctttcgcc
     8161 gtcgttcttc atcctctacg ctggagttcg cacagcagct ccatcctgcg ccgcctccag
     8221 catcggatcc tttcagagtt tcgaccttgg cgacctgctg gaccgctacg tcccgttctc
     8281 caaggccgat gcgctcggga taacgatccc gacggtcgaa gaccccagcc ttgccccccc
     8341 ggaccatgaa gtgtgtatcg tccacgaact catcccccac tgggacaccc gggattggag
     8401 actagaggag aagatctgca ctcagaaagt cctcgggaag gccgagcggg tcttcccagg
     8461 actgacgcgg cggttggcat actgccaggc cgccacgcca gccacgctgg agcggtatac
     8521 ccgcaaccgg ggtggggccg cgtatggctg ggatcagatc ccccatctcc caaaggttcg
     8581 ccacgggatc ggcaatctcc atctggctgg acactgggcg gaaactggag ggggcgttct
     8641 ggcagccgcc tactccggca tgcggatcgc ggtgcggatt ctcagggcca cctcgtgaac
     8701 ggacgggcgt cggtctgcgg gatcatcccg gcctataatg cccgagagac gattgcggga
     8761 gtcgtgcagg gagttctccg tcacctggag acggtgatcg tcgcggacga cggctccacg
     8821 gatggaacgg cggaggtcgc cagacgggcc ggcgccggtg tgatcgtgct gggggaaaat
     8881 cgagggaagg gcgacgccct ccgcatcctc tttgccgagg cccagcggcg ggggttctcg
     8941 gcagccatgg cggttgatgc cgatgggcag cacgacgtgg atgacatccc gtcgttcctt
     9001 cgggcgcatc gagactcccc ggacgccatc ctcaccggtt cgcgcatggg cgcccgggac
     9061 cacatccctc gtcaccggca caactcgatg ctcgtggcgc gctacttcat ctccctggct
     9121 gccaaccagt tcatcgagga tacccaatgc gggttccggc tgtatccctt ctcggtgatc
     9181 gggtccctct cgctgctgaa gggacggtac gtgacggaga cggagatcct gataaaggcc
     9241 ggagactcgg ggagacagat tcggtgtgtc cccatcaagg cccattatcc ccccggacag
     9301 ctgacgcact tccgctcggt cagtgacatc gccgccatct cgctgtacgt gatctcctac
     9361 ctgatggtca agtgggggat agaaggagtg cggccgggcg tgagcaatac ctacaggggg
     9421 ggaggcacgg gccgagacgt attcttcttg tcgccgtttc ttgactggat atttgaagtc
     9481 ctgacattca gcttcgcact tccgctcagc gttgcctacg gcgcctggta ctacgtggcc
     9541 cgcttcttct ccgtgcccgc cctgcagagc ctggcccgaa acagcgttcc cgtgggacgc
     9601 ttagtgtgtt ccgtcctcct gctcccggtc ttgctcatcc tctcgatcct cgatctcctc
     9661 gggcaccgca tgcgaattcc ttcggatctc acgacgccgt tcgtgcgtcg gtggtattcg
     9721 attccatatc accaggaata aacggctctc cggcgtccgg gctcctggct cagccggaag
     9781 cgggtcaagc tgcccacgaa cttcgtggcc cttgagttcg ccagcccgcc gggcgatccc
     9841 ctaggatcga ccttccgagg cattctcagc gattatttgt ttcatcggta ttcgcaccgt
     9901 gacggttgtg ccttcacggg gacgacccac gatgaagacc tggcctccga agaggcaggc
     9961 ttgctcgcgc atgatgagga gcccgagaga ggttcgactg gtgagcgcct gctcggtgat
    10021 gccctggccg tcgtcgtgga ccatcaggac gaagtgtccc gtctcctcgc ccaaacggat
    10081 gtgcacccga ctggcgcgcg cgtgccgggc aacattggtc aggcactctt ggcagatgcg
    10141 gaacagggcc gtgctcgctt cgggatccag gcggacgtct gggcgatccg aggcgaactc
    10201 acaccggatg gacgtgtgcg cctgaaattc ttgagcctgc cactcgatgg catccacgat
    10261 gcccaggtca tccaggatgc cgggacgcaa ttccgtggcc agccgccggg cggacttgat
    10321 cgcggaatcg gcgacggcca gcatggcctt cagtttccct ggcaggggcg cctgatccgc
    10381 cggcagcttg gtggccaacc aggccagatc gagcttcagg gctgtcagcg cctgcccgag
    10441 ccgctcctgc agctcctgcg ctacgcgtaa gcgttcgtcc tcccgtacca acagcaggcg
    10501 tctcgtcagg gcgcggacct gctcccggga ggcctggaga tcctcctcgg cccgcttccg
    10561 gtcggtgacg tcgcgcagga tagccgtgta gagcttccgc ccgccgaatt ccacctggga
    10621 gatggcggcc tccatgggaa attcttcccc gtccgcccga agggcggtcg cccccccgag
    10681 taacccgatc gaccaccgct ctacattcgt ccggccgaag tcgcggagac ccgccaggtg
    10741 tgtggcgcga cgctgttcgg ggatgaagcg ctgcaccggc tgtcccatga cctcggccgc
    10801 cgagacccga aacatccgct ccgcggccgc gttgaagagg acgatccgtt gcgtctcgtc
    10861 gatgctgata atggcctcgc gtgccgaccc gatgatggcc gcgagttggg cctggctctc
    10921 gcggaccgcc tcctcggcct gctgccgctg gcccaggacc gcctgaagct cctcgttcgc
    10981 ccgcgtcaga ccatcggtcc ggtcgcgaaa cccgctgtcc atctcggcct gcgcggcacg
    11041 cagcgcctcc tcggcctgtt tggtgacact gatatcccgc caattgccga cgacgccctg
    11101 cacactcggg tcgtcgagcc agctcctgat cccgaactcg atccagcgcc aggacccatc
    11161 cgtgtgccgc acgcgtaact caatccggag gctctgcccc ggatgccgca gtgcatccgc
    11221 gaaccgagcc gtggcgtttc cggagtcctc ggcatggatg aag
//
LOCUS       DBNGZC010000254         2364 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1095313, whole genome shotgun sequence.
ACCESSION   DBNGZC010000254 DBNGZC010000000
VERSION     DBNGZC010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1095313"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..>2364)
                     /locus_tag="AC16G0_04700"
     CDS             complement(123..>2364)
                     /locus_tag="AC16G0_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5098957.1"
                     /translation="QEDFPGLKFEVAYDNATFVGYLMDNMVEELVLAIGLTGVVVLLF
                     LGNLRGTLISVITIPISLAMALLAMVPFGMTLNSSTLIGLLLSIGRLVDDSIIDIHSI
                     ERHLRMGKSPKEAAVDGISEVRLAVLAITFMLCVALLPLAFSGGIVQYMFEGIVWAII
                     LALLASALVSFTLTAVLAANLFKPHTEGAAQQAGWFERTLLGPFQRFLENMEARYRGG
                     LGWAIRNRFSVLAGALAIILVGVALYPKIGSEMMPLADVSQAYAQLEATPGTSFARTS
                     EIAAQVERLLLKQPEVEKVSSEVGFEPGGTYFTGYSMGAVNAASMMITLVDSSKRKRD
                     IWQVIDEVQAEAVRTIPGIRRLAIKEMGADVMASSAAPIQVIFFGPELEKLAEIGEQA
                     RKLAEGIPGFFQVSTSWSETLPQLHVQVDRARAQELGLTVSDVADQAYYALKGGLTNE
                     FYRLDNKRQFTILLRYRGDQRRDEADLAQVKIVGKKGEVVPLSSVARIEEHRGPTLIE
                     HDNFRRVISVLGYYRKGGPPSMELSMDLLMAAYGKITFPPGYGVELRGDMTQMEESFQ
                     RLMRGLYLAVIFMFLLLVAQFRSLIEPFNMIFSLSLVTTGILGGLFLAGQTFSTVSIL
                     AVVILTGMMMTVAVLMIDLVLRLRREGMARDEAILTAGPIRLRPILMTSFISIVVLIP
                     VAFFPRTGIDAYAPLATVTIGGLSIGTLLALFVVPVIHTYTDDLARVMRVVVRKLLRR
                     GEEAAV"
BASE COUNT          456 a          786 c          755 g          367 t
ORIGIN      
        1 ggacgcctcg gcccgagccg gcaggcgcag cgtcgaatgt ctcggccatt gctggacgag
       61 gacatagccc aacccgatgg ccaccagggc gaggctcgtg aggaggtacc gggcccggat
      121 gctcataccg ccgcctcctc accccgccgg agtagcttcc ggacgaccac cctcatgacc
      181 ctagccaggt catccgtgta ggtatggatc acgggcacga cgaagagggc gaggagcgtc
      241 ccgatcgaca gccccccgat ggtcaccgtg gccagcgggg cataggcgtc gatacccgtc
      301 cgcgggaaga aggccaccgg gatcaggacg acgatgctga tgaaggaggt catgagaatc
      361 ggccgcagcc ggatgggtcc ggccgtgagg atggcctcgt cccgcgccat cccctcgcgc
      421 cggagacgca agaccagatc gatcatcagc acggccaccg tcatcatcat ccccgtgagg
      481 atcaccaccg ccaggatgga caccgtggag aacgtctgcc cggccaggaa caggccgccc
      541 aggatgcccg tggtcacgag ggagagggag aagatcatgt tgaagggctc gatgaggctc
      601 cggaactgcg ccacgagcag caggaacatg aagatcacgg ccaggtaaag gccccgcatc
      661 agtcgctgga acgactcctc catctgggtc atgtcaccgc gcagctccac gccgtagccg
      721 ggagggaagg tgatcttccc gtaggcggcc atcaagaggt ccatggacag ctccatgctc
      781 ggcggcccgc ccttccggta atagcccagg accgagatca cccgccggaa gttgtcgtgc
      841 tcgatgagcg tgggcccgcg atgttcctcg atccgggcca ccgagctcag ggggaccacc
      901 tcccccttct ttcccacgat cttcacctgc gccaggtccg cctcgtcccg gcgctggtca
      961 ccccggtagc ggagcaagat ggtgaactgc cgcttgttgt ccagacggta aaactcgttg
     1021 gtgaggccgc ctttcaaggc gtagtaggcc tgatccgcca catcgctcac cgtgagaccc
     1081 agctcttggg cgcgagccct gtccacctgg acgtggagct gcggaagcgt ctccgaccag
     1141 gaggtggaca cctggaagaa gccggggatt ccctcggcga gcttccttgc ctgctccccg
     1201 atctcggcga gcttctccag ctccggcccg aagaaaatga cctggatcgg ggcggcgctg
     1261 gacgccatga catccgcccc catctccttg atggcgaggc gccggatccc ggggatcgtc
     1321 cgcacggcct ccgcctgcac ctcgtcgatc acctgccaga tgtcccgctt gcgcttcgag
     1381 gaatccacca gggtgatcat catagatgcg gcgttgaccg cacccatgct atatccggtg
     1441 aagtaggtgc cgcccggctc gaatccgacc tcggaggaga ccttctccac ttccggctgc
     1501 ttcaggagga gtcgctccac ctgcgcggca atctctgaag tccgggcgaa ggacgtgccg
     1561 ggtgtcgcct cgagctgcgc gtaggcctgg gacacatcgg ccaggggcat catctcgctg
     1621 ccgatcttgg ggtagagcgc cacgcccacc aggatgatgg ccagggcgcc cgcgaggacg
     1681 ctgaaccggt tccggattgc ccagcccagc ccaccgcggt agcgcgcctc catattctcc
     1741 aggaatcgct ggaaggggcc gagcagcgtg cgctcgaacc agcctgcctg ctgcgccgcc
     1801 ccttcggtgt gtggcttgaa caggttggcc gccaggacgg ccgtcagggt gaaggagacc
     1861 agggccgagg cgagcagcgc caggatgatg gcccacacga tcccctcgaa catgtactgc
     1921 acgatcccgc ccgagaaggc caggggcagg agggccacgc agagcatgaa ggtgatcgcc
     1981 aggaccgcga gacgcacctc gctgatccca tccacggccg cctcttttgg cgacttcccc
     2041 atgcggaggt gccgctcgat ggagtggatg tcgatgatgg agtcatccac caaccgcccg
     2101 atggacagca gcaggccgat gagcgtcgag ctgttgagcg tcatcccgaa cggcaccatg
     2161 gccagcagag ccatagccaa cgaaatggga atcgtgatga ccgagatcag cgtgccacgc
     2221 aggttgccca gaaagaggag cacgacgacg ccggtgaggc cgatcgcaag gaccagctcc
     2281 tccaccatgt tgtccatcag gtagccgacg aacgtggcgt tgtcgtaggc cacctcgaac
     2341 ttcaggcccg gaaaatcctc ctgg
//
LOCUS       DBNGZC010000255         7662 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1095448, whole genome shotgun sequence.
ACCESSION   DBNGZC010000255 DBNGZC010000000
VERSION     DBNGZC010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7662)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7662
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1095448"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="AC16G0_04705"
     CDS             complement(<1..202)
                     /locus_tag="AC16G0_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/IscA family protein"
                     /protein_id="HIR5098958.1"
                     /translation="MITVTPLAAEKIKGIKTSQAAPEDAGLRVRVVGGGCSGFQYQLV
                     FDAPKAGDQVYEQDGVKVVVDPK"
     gene            complement(226..648)
                     /locus_tag="AC16G0_04710"
     CDS             complement(226..648)
                     /locus_tag="AC16G0_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HIR5098959.1"
                     /translation="MYSNEVLNHFENPRNMGALPDANGVGIVGAPACGDVMKLQLKIV
                     TESDGTEWIADVKAKVFGCGNAIASTSFLTELIKGKSLTEACQVRNRAIAAALALPPV
                     KIHCSVLAEDALSAAIADYRKRTIATAQPVPGGQSEQS"
     gene            complement(684..1904)
                     /locus_tag="AC16G0_04715"
     CDS             complement(684..1904)
                     /locus_tag="AC16G0_04715"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HIR5098960.1"
                     /translation="MTVKLPIYMDHHATTPVVPEVFEAMRPYFMERFGNAASRNHAFG
                     WAAESAVDAARAQIARLIGCKPVELVLTSGATEADNLAVKGVAYAYREKGNHIVTCQI
                     EHHAILDPCKRLEKEGFEVTYLPVDSYGRVDAGAVAEAITPKTILVSIMLANNEIGTI
                     QPLTEIGKVCKERGVLFHSDAVQGVGKIPSNVDTLGVDLMSLTAHKMYGPKGVGALYV
                     RMGKPRVKLVAQMDGGGHEKGRRSGTLNVPGIVGFGKACELAEQLMEGEAARLTSLRE
                     RLRAGIFARLEHVHLNGHPTERLPGNLNVSFEFVEGESLLMSLKDVAVSSGSACTSTS
                     LEPSYVLRALGIGEELAHTSIRFGLGRGNTLEEVDYVCDQLAEQVTRLRAMSPLYEMA
                     QAGIDLKSVKWNAH"
     gene            complement(1921..2400)
                     /locus_tag="AC16G0_04720"
     CDS             complement(1921..2400)
                     /locus_tag="AC16G0_04720"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR5098961.1"
                     /translation="MDNQRLLKIGEVARRTGTSLRTVRYYEELNLIQPKVRSQGGFRL
                     YTEEECQRVQLIKNLQLLDFPLAKIKELFSGRGQAATGEEAAHEILALLAEHLRETET
                     RIARYQEMKRAIEDTVGIIHECLGCHRTPAKDVCLSCENITARKRLPLPMEYLIAAS"
     gene            complement(2686..3390)
                     /locus_tag="AC16G0_04725"
     CDS             complement(2686..3390)
                     /locus_tag="AC16G0_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012612710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5098962.1"
                     /translation="MLRVTGIQTFYGDLQALHGVSFDVREGEIVSVLGSNGAGKTTTL
                     MTISGVLRPRRGQIEFDGHRIQGLKPSAIVERGLVHVPEGRQLFPSMTVEENLLMGGC
                     SPRAKAARQETLERVHHIFPRLRERRQQLAGTLSGGEQQMLAIGRGLMSCPRLLMLDE
                     PSLGLAPALVSSIFDIVRDINGAGISVLLVEQNVFRALKLSHRAYILENGRIVAEGPS
                     QQLLKDPQVRTAYLGL"
     gene            complement(3482..4228)
                     /locus_tag="AC16G0_04730"
     CDS             complement(3482..4228)
                     /locus_tag="AC16G0_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012612709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5098963.1"
                     /translation="MRLLQVEGASKSFGGLCAVHRVSFDLAEGEMLGLIGPNGAGKTT
                     LFHMVSGFLPPDEGSISFAGEPLTGLRPHDICLRGLVRTFQVARPFLRLSVLENVVVG
                     ALERVKHRRAAVGIAEEILSFTGLAEKAGSPGHSLTLPDRKRLELARALATGPRALLL
                     DEVMAGLNPTETGRLIELIRAVHARGVTILLIEHVMKAVMSLSQRVLVLSYGELIAEG
                     RPEEVVLDRRVIEAYLGEDYGGVEADDDAS"
     gene            complement(4225..5190)
                     /locus_tag="AC16G0_04735"
     CDS             complement(4225..5190)
                     /locus_tag="AC16G0_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5098964.1"
                     /translation="MTRRLALGAMGGAVLLVLPFYLSSTWQNLFITAFYYAFLGQAWN
                     ILGGYAGQLSLGHAAFFAIGAYVSAVLQTQFAISPWLGMFMGAALAALLSLGIGYLGF
                     RFGLRGFYFILLTLAAAEICRLIALHLPVLGGYTGLFIAFTPNPWQFQFRGKIPYYYI
                     ALAFLVLASGIVWMIERSKLGAYLMAIREDEDASEALGVDTFRYKMIAYAVSAALTAL
                     GGTFYAYFQFYLQPNTVLNINHSVDIMIRPIVGGSGTLLGPVLGSFLLELLGEFSRTY
                     FAGGTAGLSLVIYGLLLIVVVLFLPRGVYPTLLHAMRRRRPRGTA"
     gene            complement(5187..6050)
                     /locus_tag="AC16G0_04740"
     CDS             complement(5187..6050)
                     /locus_tag="AC16G0_04740"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5098965.1"
                     /translation="MSLYTQAIVNGLLVGGVYSLMALGLSLIFGVMRVINFAHGDMMV
                     WGMYLSYWLFVAAGVDPYLSILVTGASVFLLGAAVQKSLVNRILEAPHEMQILLMLGV
                     ALVLENTALMVFGPDPRRVGTSYALSTFWLGPIVFDIPRVATFCVALALAVGLYLFLK
                     KTEMGRMIRAASDNQVGALLVGTDILRVFTLAFGIGAACVGAAGSLMTPFLPFAPSTG
                     LFFTVTSFNIVIIGGMGSLLGAFVGGLLVGISEALGAVILVPSLKEIVSFVLLVLILL
                     FRPQGLVGSRR"
     gene            complement(6252..7517)
                     /locus_tag="AC16G0_04745"
     CDS             complement(6252..7517)
                     /locus_tag="AC16G0_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098966.1"
                     /translation="MSSRGRRAGVNRRTVLKRMGAAGAVGVLGFPAILRAQVKEIKIG
                     SIQPMTGVLAVVGKTSRQGNQLAVDHINAAGGIKSMGGAKLVLVPGDTQAKPEVARAE
                     TERLMREGVNVLVGAFNSSDIAAIIQAIQQSTKPIPLMIDIGSADQLTQMGVKYVFRS
                     FTTSSMLGRRGIEYLMEMFKTTGIECKRAVFFHTSDLFGQVGREKTLEWHKKLKAPFE
                     IAEIITYPVNTQDLSTEVSKAKAAKPDLILAITRPNDAIMLMQEMFKQRVDVKAIMGP
                     GNPGFYQPTFMRTLGKLAEYTMANAPWYNPKSPLTQKVGADYEKRFGDQLTTESAWAY
                     QGVVVIADILERAGTTDPDKFVEAARKTNIKDHITSGGSIQFDEHGDNVGASSAMVQI
                     REGRARVVFPKEFAEVQVVYPIPKLWERG"
BASE COUNT         1423 a         2541 c         2334 g         1364 t
ORIGIN      
        1 acttcgggtc cacgacgacc ttcacgccgt cctgctcgta cacctgatcc cccgccttgg
       61 gggcatcaaa gacgagctgg tactggaagc cggagcagcc gccgccgaca acccggaccc
      121 ggaggccagc atcctcgggg gccgcttgcg aggtcttgat acccttgatc ttctcggcgg
      181 caagtggggt gacagtgatc atgattacgc tcctctttct tgatgttaag actgctcaga
      241 ctgcccgccg ggcaccggct gggcggtggc aatcgttcgc ttccgataat cggcgatggc
      301 ggcactcagg gcgtcctcgg ccaatacgga acagtggatc ttcaccggtg gcaacgccaa
      361 ggcggctgcg atggctcgat tcctgacctg acaggcctcg gtcaaggatt tccccttgat
      421 cagctcggtc aggaaggaag tggaggcgat ggcgttgccg cagccgaaga ccttggcctt
      481 cacgtcggcg atccactcgg tcccatccga ctctgtcacg atcttcaact gaagcttcat
      541 gacgtccccg caggctgggg cccccacgat tcccaccccg ttggcgtcgg ggagggcccc
      601 catgttgcgg gggttctcga aatgattcag gacctcgtta ctgtacatcc gcgctcctct
      661 ggggctccgc gacccgcaag ggatcagtgg gcgttccact tgacactctt caaatcgatg
      721 cccgcctggg ccatctcgta gagcggactc atggctcgga gccgcgtgac ctgctccgca
      781 agctggtcgc aaacgtagtc cacctcctcc aaggtgtttc ctcgacccag gccgaagcgg
      841 atgctggtat gggccaactc ctcaccaatg ccgagggccc gcaggacgta gctcggctcc
      901 aacgaggtgg aggtgcaggc ggagccggag gagaccgcca catccttcaa ggacatgagg
      961 agggattctc cctcgacgaa ctcgaagctc acgttcagat tccccggaag cctctctgtc
     1021 ggatgaccgt tcaggtgcac atgctccagg cgtgcgaaga tgccggcccg caggcgctcc
     1081 cgcagggagg tgaggcgcgc agcctctccc tccatgagct gctcggccag ctcgcaggct
     1141 ttgccgaagc ctacgatgcc ggggacgttc agcgtgccgc tgcggcggcc tttctcgtgg
     1201 ccgcccccgt ccatctgggc gacgagcttc acgcggggct tccccatccg gacgtacagg
     1261 gccccgaccc ccttggggcc atacatcttg tgcgccgtga gggacatcag gtccactccg
     1321 agggtgtcca cgttggaggg gattttcccc acgccctgga cggcgtcgct atggaagagg
     1381 acgccgcgct ccttgcagac cttcccgatt tccgtcaggg gctggatcgt tccgatctcg
     1441 ttgttggcga gcatgatgga gaccaggatg gtcttgggag tgatcgcctc ggcgaccgcc
     1501 ccggcgtcaa ctctcccgta cgagtccacg ggcaggtaag tgacctcgaa gccctccttc
     1561 tccaaccgct tgcagggatc caggatggcg tggtgctcga tctggcaggt gacgatgtgg
     1621 ttaccctttt ctcgataggc gtaggccacg cccttgacgg ccaggttgtc cgcctcagtt
     1681 gcccccgagg tgagcaccag ctcaaccggc ttgcagccaa tgagccgcgc gatctgggcg
     1741 cgggcagcat ccacggctga ctcagcggcc cacccgaagg cgtggttccg agaggcggcg
     1801 tttccaaacc gctccatgaa gtacggccgc atcgcctcga aaacttcggg caccaccggg
     1861 gtcgtcgcat gatgatccat gtagatggga agcttcacgg tcatgggttt cctcaggggt
     1921 tcacgaggca gcgatgaggt attccatcgg aagcgggagc ctcttccggg cggtgatgtt
     1981 ctcgcagctc aagcagacgt ctttggcggg cgtccggtgg cagcccaggc actcgtggat
     2041 gatccccacg gtgtcctcga tagcccgctt catctcctgg tacctggcaa tgcgggtctc
     2101 ggtctcgcgc aaatgctcgg cgagcagagc caggatttcg tgagccgcct cttccccagt
     2161 cgccgcctga ccccgcccgc tgaaaagctc cttgatcttg gccagtggga agtccagcag
     2221 ttggaggttc ttgatgagct gaacccgctg gcactcctcc tcggtgtaca gacggaaccc
     2281 gccttgactc cggacttttg gctggatgag gttcaactct tcgtaatagc gaacggtccg
     2341 caggctggtc cccgtccgcc tggccacctc gccgatcttc agaagcctct ggttgtccac
     2401 gtaacccctc atcattattg caggcttgcg taaaatacca aacccttacg tgactgtcaa
     2461 gatgggattc ccctaagcgt tgagttgttt tcggaagcga ccaaacacag cgagccttcg
     2521 gcatcgtttg gaccggtctc tgcgggggga tctgcgaagg gggcacttcc cagcaaccca
     2581 aggtgatcag ccctggagtg gaacaaacca tgctggccaa acgccctgac tcctcccggg
     2641 ccgatctgcg gaatctgcga aatctgtgga taatctggat cggcactaca gaccgaggta
     2701 tgccgtccgc acctgcgggt ccttgaggag ctgctggctc ggtccctcgg ccacgatccg
     2761 gccgttttcc aagatgtaag cgcgatggga gagcttgagg gcccggaaga cgttctgctc
     2821 caccaggagg acgctgatcc cggccccatt gatatcccgg acgatgtcga agatggagga
     2881 gaccagcgcg ggtgcaaggc cgagggacgg ctcgtccagc atgagcagcc gcggacagga
     2941 catgagccct cgcccgatgg cgagcatctg ctgctctccg cccgataggg tccctgccag
     3001 ttgctgccgt cgctcccgca atcgcggaaa gatatggtgc acccgctcca gcgtctcctg
     3061 gcgggccgcc ttcgcccgag gcgagcaccc ccccatgagg agattctcct ccacggtcat
     3121 gctggggaag agctgccggc cctccggcac gtgaacgaga ccgcgctcca cgatcgccga
     3181 cggcttcagg ccctggatgc gatgcccgtc aaactcgatc tgcccgcgac gcggacggag
     3241 gacgcccgag atggtcatca aggtggtggt cttcccagct ccgttgctgc ccaagaccga
     3301 gacgatctcc ccctcccgga cgtcaaagct gaccccgtgg agcgcttgga gatcaccgta
     3361 gaaggtttgg attcccgtca ccctaagcat aggcgacaaa tcatcccagg agtttcatgc
     3421 acggcaattc tttgcgccct ttgcgggctt tgcgggcata taccaaggtc ggatggtctt
     3481 tctaagaggc atcatcatcg gcctcgacgc cgccgtaatc ctcgcccagg taggcctcga
     3541 tcaccctccg gtccagcacg acctcctcgg gccgaccctc ggcgatcagc tccccgtagc
     3601 tcaacaccag gacccgctgc gaaagactca tcaccgcttt catgacgtgc tcgatgagca
     3661 ggatcgtgac ccctcgggcg tgaaccgcgc gaatgagttc gatgaggcgg ccggtttcgg
     3721 tagggttgag acccgccatc acctcgtcca ggaggagtgc ccgtggcccc gttgccagcg
     3781 cccgggcaag ctccagacgc ttgcggtcgg ggagggtgag gctgtgtccc gggctgcccg
     3841 ccttttcggc cagcccggtg aagctcagga tctcttcggc gatgcccact gcggcccggc
     3901 ggtgcttcac ccgctccagc gcccccacca ccacgttctc cagcacgctc aggcgcagga
     3961 acggccgggc cacctggaat gtccgcacca gcccccgcag gcagatatcg tgcggccgga
     4021 gaccggtgag aggttccccc gcaaagctga tggacccctc gtcgggcggg agaaatcccg
     4081 agaccatgtg gaacagggtg gtcttccccg ccccattagg cccgatcagg cccagcatct
     4141 ccccctcggc gaggtcaaag ctgacccgat ggaccgcgca gagaccgcca aaggacttgc
     4201 tcgccccctc gacctggagc agcctcacgc ggtccccctc gggcgccgtc gtcgcattgc
     4261 gtgcagcagc gtgggataca ccccgcgcgg caggaacagc accacgacga tcagcagcag
     4321 gccgtagatc accagactca gcccggccgt ccctccggcg aaataggtcc gcgagaactc
     4381 acccagtagt tcgagcagga aggagcccag caccggcccg agcagcgtcc cgcttccccc
     4441 cacgatcggc cggatcatga tgtccacgga atgattgatg ttcagcacgg tgttgggctg
     4501 caggtagaac tggaaatagg cgtagaaggt gccgcccagg gcagtcaggg ccgcgctgac
     4561 cgcgtaggcg atcatcttgt agcggaacgt gtccaccccc agcgcctcgg aagcatcctc
     4621 atcctcgcgg atcgccatca ggtaggcccc cagcttcgag cgctcgatca tccagacgat
     4681 gcccgacgcc agcaccaaga acgccagggc gatgtagtag tacgggatct tgccccgaaa
     4741 ctggaactgc catggattcg gcgtgaaggc gatgaagagg cccgtgtacc cgcccaggac
     4801 ggggagatgg agagcgatga ggcgacagat ctccgccgcc gccagcgtga gcaggatgaa
     4861 gtagaagccg cgcaggccga accggaaacc caggtacccg atccccaggc tcaagagcgc
     4921 cgccagggcc gcccccatga acatccccag ccagggagag atggcgaact gggtctgcag
     4981 gaccgccgag acgtaagcgc cgatggcaaa gaaggcggcg tgacccagcg agagctggcc
     5041 cgcatacccc ccaaggatgt tccacgcctg acccaggaag gcatagtaga aggcggtgat
     5101 gaacaggttc tgccacgtgg aggacaggta gaacggcagg accaggagga ccgcccctcc
     5161 catcgcaccc agagccagcc gccgcgtcac cgccggctcc cgacgaggcc ctgggggcgg
     5221 aacaacagga tcaggaccag caggacgaag ctgacaatct ccttcagcga gggcaccagg
     5281 atcacggcgc ccagggcctc actgatcccc accaagaggc cgcccacgaa agctcccagc
     5341 aggctcccca ttcccccgat gatgacgatg ttgaaggagg tgacggtgaa gaagagtcct
     5401 gtgctcggcg cgaaggggag gaacggcgtc attagactgc cggcggctcc cacgcaggcc
     5461 gccccgatgc cgaaggccag cgtgaagact ctcaggatgt ccgtgcccac caggagcgca
     5521 cctacctggt tgtcggacgc ggcgcggatc atccgtccca tctcggtctt cttcaggaac
     5581 aggtagagcc ccacggccaa agccagggcc acgcagaagg tggctacccg gggaatgtcg
     5641 aagacgatcg gccccagcca gaacgtgctg agggcgtaac tggtgccgac gcggcggggg
     5701 tccggtccga agaccatcaa ggcggtgttc tcgaggacca gggcgacccc cagcatcagc
     5761 aggatttgca tctcatgggg ggcctccagg atccggttga ccaggctctt ctggaccgcc
     5821 gcgcccagca gaaagacgga tgccccggtc accagaatcg agaggtaggg atcgactccc
     5881 gccgccacaa acagccagta ggacaggtac atgccccaga ccatcatgtc gccgtgggcg
     5941 aagttgatga cccgcatgac gccaaagatc aacgacaggc ccagagccat gaggctgtag
     6001 accccgccga ccagcagccc gttgacgatc gcctgcgtgt acagcgacac cgcgccccca
     6061 gaaacatgaa ggaggcagaa ggacgctcgc ccccctgcct cctgccgctt gccatccggt
     6121 ccctagtccc tcgtccccaa cccctgcccc catcgaatct cgaaccgtac attcccttcc
     6181 gtcgagtttg ttctccccac tcggcgctcg cctctcagct ctctgcgctc ggcgctctgc
     6241 tctcggcgcc cttacccccg ctcccagagc ttggggatcg gatacaccac ctggacttcg
     6301 gcgaactcct tggggaaaac gacgcgcgcc cgtccctcgc ggatctggac catcgccgag
     6361 gaggcgccca cattgtcccc atgctcgtcg aactggatgg agcccccgga cgtgatgtga
     6421 tccttgatgt tggtcttgcg ggccgcctcg acgaacttgt ccgggtccgt cgtcccggcg
     6481 cgctccagga tgtcggcaat cacgaccacg ccctggtagg cccaggcaga ctcggtggtg
     6541 agctggtcgc cgaatcgctt ctcgtaatcc gcgccgacct tctgcgtcag tggcgatttg
     6601 ggattgtacc agggggcgtt cgccatggtg tactccgcca gcttgccgag ggtccgcatg
     6661 aaggttggct ggtagaatcc gggattgccc ggacccatga tggccttcac gtcgacgcgc
     6721 tgcttgaaca tctcctgcat cagcatgatg gcgtcgttgg gacgcgtgat cgccaggatg
     6781 aggtccggct tcgccgcctt cgccttggaa acctccgtgg acaggtcctg cgtattgacg
     6841 gggtaggtga tgatctcggc aatctcgaaa ggagccttca gcttcttgtg ccactccaga
     6901 gtcttctccc gtccgacctg gccgaagagg tcgctggtgt ggaagaacac cgcccgcttg
     6961 cattcgatgc ccgtcgtctt gaacatctcc atgaggtatt cgatcccgcg ccggcccagc
     7021 atcgaggagg tggtgaagga tcgaaagacg tacttcacgc ccatctgcgt cagttgatcg
     7081 gcggacccga tgtcgatcat cagggggatg ggcttcgtgg attgctggat cgcctggatg
     7141 atggccgcga tgtcgctgct gttaaacgct cccaccagca cattgacgcc ctcgcgcatg
     7201 agacgttcgg tctcggcccg ggcgacctcg ggtttggcct gcgtgtcccc cgggaccagg
     7261 accaatttgg ccccacccat ggacttgatc ccgccggcag cgttgatgtg atccacggcc
     7321 aactggttgc cctgtcgaga ggtcttcccc accaccgcca ggacgcccgt catcggctga
     7381 atggagccga tcttgatttc tttgacctgg gcccggagta tcgcgggaaa gcctaaaacg
     7441 cccacggccc ccgctgcccc cattctcttc agcaccgtcc gccggttcac cccggcccgc
     7501 cttcctcgcg acgacattgg ctcgcctccc cttctcagta agtatccgat ggcacaagca
     7561 aacaggccga cgatatacca ggatttgcca ggtcgagcaa gggctttcgt agctctcgcg
     7621 gaagtggcgt gacgagaatc ctcgccaggg tcggtgaaat ag
//
LOCUS       DBNGZC010000256         2412 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2270822, whole genome shotgun sequence.
ACCESSION   DBNGZC010000256 DBNGZC010000000
VERSION     DBNGZC010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2270822"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /gene="rpmD"
                     /locus_tag="AC16G0_04750"
     CDS             <1..213
                     /gene="rpmD"
                     /locus_tag="AC16G0_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR5098967.1"
                     /translation="GQLKIRLRRSRIGTTPRQREVLRGLGLRRIDSQVLREDTPSIRG
                     MLNKVGHLVEVEPVADGTPTAGDGEA"
     gene            210..653
                     /gene="rplO"
                     /locus_tag="AC16G0_04755"
     CDS             210..653
                     /gene="rplO"
                     /locus_tag="AC16G0_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR5098968.1"
                     /translation="MKLHELKAPRGSRERRKIVGRGPGSGHGKTSGRGEKGQKARAGG
                     SSQPWFEGGQLPLHRRIPKRGFVNIFRLECAVLNVKDLERFDAGTAVTPATLREAGLV
                     RAKAAVKILGEGSLSKPLTVSAHRFSKSAAEKIRAAGGTVEVIAS"
     gene            650..1957
                     /gene="secY"
                     /locus_tag="AC16G0_04760"
     CDS             650..1957
                     /gene="secY"
                     /locus_tag="AC16G0_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR5098969.1"
                     /translation="MIEQVRNIFRVPELKRRVLFTCALLIVYRIGAHVPTPGIDAHAL
                     ALFFQQQAGNLLGFFDLFSGGALRRLSVFALGIMPYISASIILQLLAVVVPALEKLQK
                     EGEQGRKKITQYTRYGTVGLAAIQGLGIAVGLESMQIKGAMVVPHPGWAFRLMTTITL
                     TAGTIFIMWLGEQISERGVGNGISLLIFAGIIVRLPEAIFASWTLITQGEMNPLVFLL
                     LLVVMVAVVAGVIVMTLGQRKIPVQYAKRVVGRRVYGGQSTHIPLRVNTAGVIPVIFA
                     SSIIAFPATVTQFIRHPWAQMVSNWLRFGGVPYTLIYAAGIIFFTYFYTAIIFNPSDV
                     ADNMKKYGGFIPGIRPGSKTAEFIERVLDRITLVGALYLTGISILPEILITQMNVPFY
                     FGGTSLLIVVGVALDTVQQVESHLLMRHYEGFLKKAKVRGRIS"
     gene            1996..>2412
                     /locus_tag="AC16G0_04765"
     CDS             1996..>2412
                     /locus_tag="AC16G0_04765"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase family protein"
                     /protein_id="HIR5098970.1"
                     /translation="MRLILLGPPGAGKGTQAKMLVERLGVPQISTGDMLRAAVKAGSP
                     LGIEAKGYMDRGALVPDQVINGLMRERVQQPDTTRGYILDGYPRTLAQAESLREMLLA
                     LGLAVHHVVSLDVPTEELVERLAGRRTCLNCGAMYHV"
BASE COUNT          395 a          762 c          846 g          409 t
ORIGIN      
        1 gggcaactca agatcaggct gcgccgtagc cggatcggca ccacgcccag gcagcgggag
       61 gtgttgcgtg gactcgggct ccgccggatc gactcccagg tcctgcggga ggacacgccc
      121 tccatccgcg gcatgctgaa caaggtaggc cacttggtcg aggtggaacc ggtcgccgac
      181 gggacgccca cggcaggaga cggagaggca tgaagctgca tgagctgaag gccccccggg
      241 gatctcggga gcggcgcaag atcgtgggaa ggggccccgg gtcgggccac ggcaagacct
      301 ccgggcgcgg cgagaagggg cagaaggcgc gagctggagg aagcagtcag ccctggttcg
      361 agggcgggca actgcccctg caccgccgca tccccaagcg cgggttcgtc aatatctttc
      421 gcctggagtg cgcggtcctg aacgtgaagg acctggagcg gtttgatgcc ggcacggccg
      481 tgacaccggc caccctccgc gaggcggggc tggtgcgggc caaggccgcg gtgaagatcc
      541 tgggagaggg cagcctgagc aagcccctga cggtctcggc gcatcgcttc tcgaagagcg
      601 cggcggagaa gatccgtgca gcgggcggca cggtcgaggt gatcgcgtcg tgattgagca
      661 ggtccggaac atcttccgcg ttcccgagct gaagcgccgg gtgctgttta cctgcgccct
      721 cctgatcgtc tatcggatcg gggcccacgt ccccaccccg ggcatcgacg cccatgccct
      781 ggccttgttc ttccagcagc aggcgggaaa cctcctcggc ttctttgacc tgttttcggg
      841 cggggccctg cgccgcctgt cggtgttcgc cctgggcatc atgccctaca tctccgcctc
      901 catcattctc cagctcctgg ccgtggtggt tccggccttg gagaagctgc agaaggaggg
      961 cgagcaaggt cggaagaaga tcacccagta cacccggtac gggaccgtgg ggctggcggc
     1021 catccagggg ctggggatcg cggtggggct ggagagcatg cagatcaagg gcgccatggt
     1081 cgtgccccat ccgggctggg ccttccgcct gatgaccacc atcacgctga ccgccgggac
     1141 catcttcatc atgtggctgg gcgagcagat cagcgagcgg ggtgtgggca acggcatctc
     1201 cctgctcatc ttcgcgggca tcatcgtcag actacccgag gccatcttcg cctcctggac
     1261 gctgatcacc cagggcgaga tgaaccccct ggtgttcttg ctgctcctgg tggtgatggt
     1321 ggccgtggtg gccggggtca tcgtcatgac cttgggccag cggaagatcc cggtccagta
     1381 tgccaagcgg gtggtggggc gccgggtcta tggcgggcag agcacccaca tcccgctgcg
     1441 agtgaacacg gcgggcgtga tcccggtgat cttcgccagc tccatcatcg ccttcccggc
     1501 gaccgtgacc cagttcatcc ggcacccatg ggcccaaatg gtatccaact ggctccgctt
     1561 cggcggcgtc ccctacacgc tcatctatgc cgcggggatt atcttcttca cctacttcta
     1621 caccgccatc atcttcaatc ccagcgatgt ggcggacaac atgaagaagt acggggggtt
     1681 cattccgggc atccggccgg gcagcaagac ggccgagttc atcgagcgcg tcctggaccg
     1741 gatcaccctg gtgggagcct tgtacctgac cgggatctcc atccttccgg agattctgat
     1801 cacccagatg aacgtgccgt tctacttcgg cggtacctcg ctcctgatcg tggtcggggt
     1861 ggccctggat acggtgcagc aggtcgagtc ccacctgctg atgcgacact acgaagggtt
     1921 cctgaagaag gcgaaggtcc ggggacgcat ctcgtgacgg gcgtgcctcg agccgagtct
     1981 ccagggcagc agggtatgcg cctgatcctt ctggggccgc cgggggccgg gaaggggacg
     2041 caggcgaaga tgctggtgga gcgcctgggg gtcccgcaga tctccaccgg ggacatgctg
     2101 cgggccgccg tgaaggcggg gtcgcccctg gggatcgagg ccaaggggta catggatcgg
     2161 ggggccctgg tcccggacca ggtgatcaac gggctgatgc gagagcgcgt gcagcagccc
     2221 gacactacgc ggggctacat cctggacggc tatccccgga cgctggccca ggccgaatcg
     2281 ctgcgggaga tgctcctggc cctggggctc gccgtgcatc acgtggtgag cctggacgtg
     2341 cccacggagg aactggtcga gcggctggcg ggtcgccgca cctgcctgaa ctgcggggcc
     2401 atgtaccacg tg
//
LOCUS       DBNGZC010000257         2211 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_901407, whole genome shotgun sequence.
ACCESSION   DBNGZC010000257 DBNGZC010000000
VERSION     DBNGZC010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_901407"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="AC16G0_04770"
     CDS             complement(<1..859)
                     /locus_tag="AC16G0_04770"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HIR5098971.1"
                     /translation="MTQVVTSADLSLLAPEIALTLAGLVVLVLDFVVRSRDRGWLAGL
                     SVLGVMVALTLVLRQGAGPERTVGFSGLHVVDHFALFFKAVFLLSTVIVILLSSRFLR
                     EAAAEGGEYYAILLFAALGTMLMASANDLLTLFIGLETLAICSYVLCGYLKRDQRCNE
                     GALKYLLMGSFSSGVILYGMVLLYGFAGSTNLPAIAGAVARANLGNPALLMAMVLLAA
                     GFGFKIAAVPFHMYLPDMYEGAPTPVVAFMAGAAELAGMAVLVRVYLLALPGLQLDWS
                     LLFWVVAVLT"
     gene            complement(856..>2211)
                     /locus_tag="AC16G0_04775"
     CDS             complement(856..>2211)
                     /locus_tag="AC16G0_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048039 - ubiquinone binding [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 4 family protein"
                     /protein_id="HIR5098972.1"
                     /translation="KEYYIFLLILETGMLGVFLALDFFLFYVFWEVMLVPMYFLIGVW
                     GSDRRLYSAIKFFLYTLFGSVVMLLGILAVYFYAGSQAGHYTFDVLELMRVGYPNTPI
                     FTLLGVPFSFQSLVWLAFGLSFAIKVPMFPFHTWLPDAHTDAPTAGSVILAGVLLKMG
                     TYGFLRFNLPMFPEASQQFVPLVFVLSIIAIIYGAMVCMVQPDMKRLIAYSSVSHMGF
                     VMLGMFAFNLQGVQGSLLQMINHGLSTGGLFLVVGLIYDRRHTRLISELGGLSRQMPV
                     YATLFAIIMLSSMGLPGLNGFIGEFLILIGAFQVRWWWGAFAVTGIILGAAYMLWLYQ
                     RTMFGEITKEENKTLPDLSFREVSTLIPIIAFCFFIGLYPAPFLKAMEVSVANVIQTV
                     EKGAAGKALGARAMAGHSGTSALRDSGARDPQVAQFSNRLIARLRDGSIARLQDHEVA
                     P"
BASE COUNT          489 a          706 c          718 g          298 t
ORIGIN      
        1 tggtgagaac cgcgaccacc cagaacagga gactccagtc gagctggagg ccgggcaggg
       61 ccagcaggta gacccgcacc aggaccgcca tgcccgccag ctccgccgcg cccgccatga
      121 aggccaccac cggggtgggc gccccctcgt acatgtcggg gaggtacatg tggaacggaa
      181 ccgccgcgat cttgaacccg aatcctgccg ccaggaggac catggccatg aggagggccg
      241 ggttcccgag gttcgccctc gccactgcgc ccgcgatggc cgggaggttg gtgctccccg
      301 caaagccgta caggaggacc atcccgtaaa ggatcacccc tgacgagaac gaccccatca
      361 ggaggtactt gagggccccc tcattgcacc gctggtcccg tttgaggtac ccgcagagaa
      421 cataggagca gatcgccagg gtctccagac cgatgaacag cgtgagcagg tcgttggccg
      481 aagccatgag catggtccca agggcggcaa aaagcaggat ggcatagtac tcgccaccct
      541 ccgccgccgc ctcgcgaagg aaccggctgg agaggagaat cacaatcacc gtgctcagga
      601 ggaacacggc cttgaagaag agggcgaagt gatccaccac gtgcaggccg ctgaatccca
      661 cggtccgctc cggcccggcc ccctggcgca acaccagggt cagcgcgacc atcactccca
      721 gcaccgagag gccggccagc cagccgcggt cacggctgcg caccacgaaa tccaggacca
      781 ggaccacgag gcctgccaag gtgagggcga tctccggtgc cagcagggag aggtccgcgg
      841 aggtgaccac ctgggtcatg gcgcaacctc atggtcctgc aaccgagcga tcgagccatc
      901 gcgcagtctg gcaatcaggc gattcgagaa ctgggcaacc tgcgggtccc gagcgccgga
      961 gtctcggagc gccgaggtgc cggaatgccc agccatggcc ctggccccca gcgcctttcc
     1021 cgccgccccc ttttccaccg tctggatgac gttggccacc gagacctcca tggccttgag
     1081 gaacggcgcc gggtagaggc cgatgaagaa gcagaaggcg atgatgggaa tcagcgtcga
     1141 gacctcgcgg aagctgaggt cgggaagcgt cttgttctct tccttggtga tctcgccgaa
     1201 catcgtccgc tggtacagcc agagcatgta ggcggcgccc aggatgatcc ccgtgacggc
     1261 gaaggcgccc caccaccacc ggacctggaa ggccccgatg aggatcagga actccccgat
     1321 gaacccgtta agccccggca gccccatgga ggagagcatg atgatggcga agagcgtggc
     1381 atacaccggc atctgccggg agagcccgcc cagctcggag atcagccgtg tgtgcctccg
     1441 gtcgtagatc agccccacca cgaggaagag gccgcccgtg gaaagcccgt ggttgatcat
     1501 ctgcaggagg ctgccctgga ccccctgcag gttgaaggcg aacatgccca gcatcacgaa
     1561 ccccatgtgg ctcacggagc tgtaggcaat cagccgcttc atgtcgggct ggaccatgca
     1621 caccatggcc ccgtagatga tggcgatgat ggacaggacg aagaccagcg gcacgaactg
     1681 ctggctggcc tcggggaaca tgggcaggtt gaaccgcagg aagccgtagg ttcccatctt
     1741 taggaggacc cccgccagga tcaccgagcc ggccgtcggg gcgtcggtat gggcgtccgg
     1801 gagccaggtg tggaagggga acatgggaac cttgatggcg aaggacaggc caaaggccag
     1861 ccagacgagg gactggaagc tgaagggcac acccaggagc gtgaagatcg gcgtgttcgg
     1921 gtagcccacc cgcatcagct ccagcacatc gaaggtgtaa tgcccggcct ggctgccggc
     1981 gtagaagtag accgccagga ttcccaggag catcaccacg ctgccgaaca gcgtgtacaa
     2041 gaagaacttg atggccgagt agaggcgccg gtcgctgccc cagaccccga tgaggaaata
     2101 catcggcacc agcatgacct cccagaacac ataaaagagg aagaagtcca gggccaggaa
     2161 cacccccagc atcccggtct ccaggatgag caggaagatg tagtactcct t
//
LOCUS       DBNGZC010000258         1963 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1977014, whole genome shotgun sequence.
ACCESSION   DBNGZC010000258 DBNGZC010000000
VERSION     DBNGZC010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1977014"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..1089
                     /locus_tag="AC16G0_04780"
     CDS             154..1089
                     /locus_tag="AC16G0_04780"
                     /inference="COORDINATES: protein motif:HMM:NF022077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HIR5098973.1"
                     /translation="MKKYKDLLGDTDSGLVSQLQDQTFRLHRRMATIRHKVAILSGKG
                     GVGKSTLTSNLAACLAEDGARVAVLDADLNGPALAKMLGVRGAPLRMSKEGAEPPEGP
                     LGIKVLSMDHLLPRDEVPLQWKAPTQQANFVWRASAEAAALREFLSDTAWGPLDILLI
                     DLAPGTDKISSLASLLPELAAAVLVTIPTEVSHLVVRKSISLAAELWGSSKVRVVENM
                     AAYCCARCGHEEPLFQGPDSAAMARELGVGFLGRIPFDPRLAAAADRGVPYVRQESGT
                     PCAGALRAVAAALQSFVENGEQGSPAALESDDPSR"
     gene            1138..1680
                     /locus_tag="AC16G0_04785"
     CDS             1138..1680
                     /locus_tag="AC16G0_04785"
                     /inference="COORDINATES: protein motif:HMM:NF019968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCP reductase family protein"
                     /protein_id="HIR5098974.1"
                     /translation="MKLERVEEPEDGSLSVVFACACGNRTAMLTNPMETQLVRALDVR
                     IGGRPSPAEPMEFVRSMLASGREAGPAATGARAPASGEASSSRCPYSAMLNLAPGRSG
                     IAGEPENRSIGESGNGMQAPASPDSRIVWSPEADRRLERIPEFIRPMARRGVEQFAAE
                     RGYRRITEDVMEEARGVLGM"
     gene            1776..>1963
                     /locus_tag="AC16G0_04790"
     CDS             1776..>1963
                     /locus_tag="AC16G0_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098975.1"
                     /translation="MPPVEECCFEKWHRSLGAGIWRRAGVLSWTILLLTAVVAWAQAG
                     GGEPSPAYRNFPLFGSRLV"
BASE COUNT          342 a          591 c          683 g          347 t
ORIGIN      
        1 ggcgggagcg aggcgaggga cgaacagagc aatatcgaat gtcgaacacc gaatgacgaa
       61 tctcgaacgg ggacggcatc ctggggatgc ttcgcctccg cgttcttcat tcgacacccg
      121 gaattcagca ttggatattg gctggtacgg accatgaaga agtacaagga ccttctgggc
      181 gataccgact cgggtctggt gagccagctc caggatcaga cattccggct ccacaggcgg
      241 atggccacga tccggcacaa ggtggcgatc ctgagcggca agggcggcgt tggcaagagt
      301 accctcacgt ccaatcttgc agcttgcctg gcagaggacg gcgcgcgggt ggccgtcctg
      361 gacgccgatc tgaacggtcc cgccctggcc aagatgctgg gagtgcgggg cgctcccctg
      421 cggatgagta aagagggagc tgagcccccc gagggaccgc tggggatcaa ggtcctctcc
      481 atggaccacc tgctgcctcg agacgaggtc ccccttcagt ggaaggcccc tacccagcag
      541 gcgaactttg tctggcgagc cagcgcggag gctgcagccc tgcgcgagtt cctgagtgat
      601 actgcgtggg ggccgctgga tatcctcctg attgaccttg cccctggcac ggacaagatt
      661 tctagcctcg ccagcctgct tcccgagctg gccgcggccg tcctggtgac cattcccacg
      721 gaggtgtctc acctcgtggt ccggaaatcc atcagcctgg cggcggagct gtggggatca
      781 tcgaaggtgc gggttgtgga gaacatggcc gcctactgct gcgcccggtg cggccacgag
      841 gagccgctgt tccagggacc ggattcggca gccatggccc gtgagttggg ggtaggcttc
      901 ctgggccgga tcccattcga tccccgcctg gcggcggcgg ccgatcgcgg tgtgccctac
      961 gtccgacaag aatccgggac gccctgcgcc ggcgctctcc gggctgtggc cgcggccctg
     1021 cagtccttcg tggaaaatgg ggagcaaggg tctcccgcgg ccctggaatc cgatgaccct
     1081 tcgcggtgaa ggaaggggag gcgaatgaaa ttcctgtgtg tccgatgcga cgaggcgatg
     1141 aagctggaac gagtggaaga gcccgaggac ggctccctca gcgtcgtgtt cgcctgcgcc
     1201 tgtggaaacc gaacagccat gctcacaaat cctatggaga cccagctcgt tcgtgccttg
     1261 gacgtgagga tcgggggacg accttccccg gcggagccca tggagttcgt gcggagcatg
     1321 ctggcttccg ggcgcgaagc tgggccggct gccaccggtg caagagcgcc tgcaagcggg
     1381 gaggcctcct cgtcgagatg cccttactcc gccatgctga acctggcgcc agggagatcc
     1441 ggcattgcgg gggaaccgga aaaccggagc atcggtgaat cggggaatgg gatgcaggct
     1501 cccgcatcgc cggacagtcg gatcgtctgg tcgccggaag cggaccgcag actcgagcgc
     1561 attccagagt tcatccggcc gatggcccgc cggggcgtcg agcagttcgc tgccgaacgc
     1621 gggtaccggc gcatcacgga ggatgtgatg gaggaggcgc gaggcgttct gggcatgtag
     1681 tcccaccctc gaccgcgcga gagcgccggt ctcgaccggc ctctccgagc tgatccgtta
     1741 gaagatatcc ggctcacccg gaggagataa gttcgatgcc gcctgtagaa gagtgctgtt
     1801 tcgagaaatg gcatagatcc cttggtgcag ggatttggcg gcgggcaggc gttctgtcct
     1861 ggacgattct cctcctgacg gctgtcgtgg cttgggccca ggcgggtggc ggcgagccct
     1921 cgcccgccta tcggaacttt ccgttgttcg gcagtcgctt ggt
//
LOCUS       DBNGZC010000259         4180 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2661902, whole genome shotgun sequence.
ACCESSION   DBNGZC010000259 DBNGZC010000000
VERSION     DBNGZC010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2661902"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="AC16G0_04795"
     CDS             complement(<1..271)
                     /locus_tag="AC16G0_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011766587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR5098976.1"
                     /translation="MSVSPRDPERILRRLEWTVIRRLDGAVHGVYRTLFRGFGLDLAD
                     LREYQYQDDVRRIDWNVTARLQTPYVREFNEDREVTAWFLLDLSPS"
     gene            complement(246..1265)
                     /locus_tag="AC16G0_04800"
     CDS             complement(246..1265)
                     /locus_tag="AC16G0_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012355671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5098977.1"
                     /translation="MDTFDSERGAQSSFMERILYEVKKVLVGQDHFLERVLVAIMARG
                     HLLVEGVPGLAKTLTVKTLARTIRGSFKRIQFTPDLVPADIMGTRIYNQKTGDFATSL
                     GPVFTNLLLADEINRAPAKVQSALLEVMQEYQVTIAGETHMVPEPFLVMATQNPIETE
                     GTYPLPEAQVDRFMMKVLVDYPSEEEEFVIVDRVTGLAEPVVAAVATTEQLGLLQREC
                     RSVYVEPSLMQYAVRLAATTRNPERYGLAELSKFLSFGASPRAPIHLIEGARALAFLR
                     GRSYALPEDVTDLVPDVFRHRLVLTYEALAEGMTADALILRIMQRIPAPEKPLEAHVR
                     IAAGS"
     gene            complement(1339..2304)
                     /locus_tag="AC16G0_04805"
     CDS             complement(1339..2304)
                     /locus_tag="AC16G0_04805"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR5098978.1"
                     /translation="MRRETLYSRSSRSHPLGRPSPEKPPDDRVAPPSDSVDQRRGSPP
                     AEAHRWHSRLPVPKIVKRHERPVLLAAGGLLALLLVALHAAMQPPAREITQGDIDAAV
                     LHTLETKPLPSAAARAYEIIRPSVVRVRGLGHHDSEKDADTETGVGSGVVIVDKGIIL
                     TNVHVVAGARRVKVVFADGLESDATVVGLQPEQDLAVLQAATIPDDLVPATLRSTSDL
                     APGDEVIAVGFPFGIGPTVSAGVVSGLRREFLSPQGKQLLTNLIQFDAAANPGSSGGP
                     LVTTDGAVVGIVTAILNPTAQRVFVGIGFAVPIESAASAVGIPPF"
     gene            complement(3082..3822)
                     /locus_tag="AC16G0_04810"
     CDS             complement(3082..3822)
                     /locus_tag="AC16G0_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007525183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HIR5098979.1"
                     /translation="MIDLGWLGQIEFTWAFFSAIMSIVLIDLVLAGDNAVVIAMAVKN
                     LSARQRLWGIALGSGGAVIIRVLSTFLVAQLLVMPYVKLIGGAVILWIAVKLLTQGAT
                     ERTHAQREAAGLWQALWIIVVADLSMGIDNMLAVGAASHGSMLLLLFGLGLSIPFVVF
                     MSNMLSKLMDRYPIILWVGAGILGKVGGQMVITDPWIQGFFPAPAWSVYLIEAIGIAF
                     VCGLAKLLLLKRRAAARMSMGASVEPAV"
     gene            complement(3882..>4180)
                     /locus_tag="AC16G0_04815"
     CDS             complement(3882..>4180)
                     /locus_tag="AC16G0_04815"
                     /inference="COORDINATES: protein motif:HMM:NF024845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HIR5098980.1"
                     /translation="QCPRCGVVLSERTRGGVVVDACCQCRGVWLDRGELEKLVAEFQA
                     HVRESRPWRAAPPSMKNADVPSRRRAGTWSRWTSRFRYPRRHPKRRLGPIDFSS"
BASE COUNT          765 a         1365 c         1366 g          684 t
ORIGIN      
        1 tggaggggct caggtccagc aggaaccagg cggtgacctc gcggtcctcg ttgaattccc
       61 gcacataggg cgtctgaagg cgcgcggtga cgttccagtc gatgcgccgg acgtcgtcct
      121 gatactgata ctcgcgcaga tcggccagat ccaggccgaa gcctcggaac agcgtccggt
      181 agacgccgtg gaccgccccg tcgaggcgac ggatcaccgt ccactccagg cggcggagga
      241 tgcgctcagg atcccgcggc gatacggaca tgggcttcca gcggcttctc gggggccggg
      301 atgcgctgca tgatgcggag gatcagtgcg tcggctgtca tcccctcggc gagagcctcg
      361 taggtgagaa ccagtcggtg gcggaagaca tcgggcacca ggtccgtcac gtcctcgggc
      421 agggcgtagc tccgtccgcg taggaaggcc agcgcccgtg ccccctcgat gagatggatg
      481 ggcgcccggg ggctggcgcc gaaagacagg aacttcgaca gctccgcgag gccgtagcgc
      541 tccggattcc gagtagtggc ggccagccgc accgcgtact gcatgagcga cggctcgacg
      601 tacacgctcc ggcattcgcg ctgcagcagg ccgagctgct cggtcgtcgc caccgcggct
      661 accacgggct ccgcgagacc tgtgacccgg tcgacgatga cgaattcctc ttcctcgctc
      721 gggtagtcca ccagcacctt catcatgaat cggtccacct gggcctcggg cagcgggtag
      781 gtgccctcgg tctcgatggg attctgcgtc gccatcacca ggaacggctc cgggaccatg
      841 tgggtttccc cggcgatggt gacctggtac tcctgcatca cctcgagcag cgcgctctgc
      901 accttcgcgg gcgcgcggtt gatctcgtca gccaagagga gattggtgaa gaccgggccg
      961 agcgatgtgg cgaagtcgcc ggtcttctgg ttgtagatgc gggtccccat gatgtccgca
     1021 gggacgaggt ccggcgtgaa ctgaatgcgc ttgaacgagc cgcggatggt cctggccagc
     1081 gtcttcacgg tcaaggtctt ggccagcccc ggcacgccct cgaccagtag gtgcccccgc
     1141 gccatgatgg ccacgagcac gcgctccaga aaatgatcct ggccgacgag gaccttcttc
     1201 acctcgtaga ggatgcgctc catgaaggag gactgcgcgc cgcgctccga atcgaaggtg
     1261 tccatgggtc ctttccgatg ctgacgccgt gtcaagacga cctggagaat tgacggggtc
     1321 gttcgtgaat actccgagct agaacggcgg tatccccacg gcggatgccg cgctctcgat
     1381 gggcaccgcg aacccgatgc cgacgaacac gcgctgcgca gtcgggttca agatggccgt
     1441 gactatgcca accaccgcgc cgtcggtggt caccagcggc ccgccggagc tgccgggatt
     1501 ggccgccgca tcgaactgga tgagattcgt aagcaactgc ttcccctgag gagacaggaa
     1561 ctcgcggcgc aggcctgaca ccacgcccgc cgacaccgtc ggcccgatgc cgaacgggaa
     1621 gccgacggca atcacctcgt cccccggcgc gaggtccgag gtcgagcgca gggtggcggg
     1681 gacgagatcg tcgggaatcg tcgccgcctg gagcaccgcc agatcctgct ccggttgcag
     1741 cccgaccacc gtggcgtcgg actcgagccc gtcggcgaac accaccttca cccgcctggc
     1801 gccggcgacg acgtggacgt tggtcaggat gatccccttg tcgacgatga cgaccccgct
     1861 gccgaccccc gtttctgtgt ccgcgtcctt ctcgctgtcg tggtgtccca agcctcgcac
     1921 gcgcacgacg gaaggtcgaa tgatctcgta ggcgcgcgcc gcggccgaag gcagaggctt
     1981 tgtctccagc gtgtgcagca cggcggcgtc gatgtctccc tgggtgatct cgcgcgctgg
     2041 cggctgcatc gctgcatgga gagccacgag gagtaaggca aggagcccgc cggctgcgag
     2101 aagcaccggc cgctcgtggc gtttgacgat cttcgggacc gggaggcggg agtgccaacg
     2161 gtgcgcctct gcgggagggc tgccgcgtcg ctggtcgacg ctgtcggacg gtggggccac
     2221 ccggtcgtcc gggggctttt ccggggatgg ccgaccaagc ggatgcgacc ggctggagcg
     2281 gctgtagagc gtttcccttc tcatgctggg tccatccttt tcacccgccg ccacccaccc
     2341 ggctcatccc ggaggaaggt gtggcaggga ggaattcgct catttgctgg cgagcgcctt
     2401 gcccaacacg catgacgcca aattcccggg agagtcagcc cccggcagcg atcgcttttc
     2461 ttggtgcgcg tcattatagc cgcctattcg ctttacagga agtgccccgt atcacaatac
     2521 gtagaatgtt gccaagaggt tcaccgtcga gccattagag atgttaccct cagggtcagg
     2581 cacggaagca gggaacgatc gtacgtcgtc aaaaggatgg aagtacacga gggcgatcca
     2641 cccgcgagtg gcgggctcca tgggaacaac tgaggggatc ggctgcaagc cgaggcggag
     2701 cttggaggcg aaggaactcc accggctgag tcgtcagaag ggcagcggga agcgagactg
     2761 ttccgtgtgg tctaccaggc cctcggtttc cttgccggat cggtcgtcat caggtttgtg
     2821 atactgcggc gaaagccggc ccgtcctgta caacggcccc gtcgatgatc tattcacccg
     2881 ggtacaggcg tccttgcgcg aggcgacccg gccggtccag caattcgggg tacacaccgt
     2941 ggcgcacacg accgttgacg acccggcgtc cgggatagtc gcccatgcct gagccccggg
     3001 agtcgcaaca gcaggcgcca cggcagaagc caagtacgta ccgggcggga cacccccgaa
     3061 cggcgtggca tgggccgcat ctcagactgc gggctcaacg ctcgccccca tggacatccg
     3121 cgctgccgcc cgtcgcttca acaggaggag tttcgccaga ccacagacga aggcaatacc
     3181 gatcgcctcg atgaggtaca ccgaccaggc tggtgccggg aagaaaccct ggatccaggg
     3241 gtcggtgatc accatctgtc ccccgacttt gcccaagatg ccggctccca cccacaggat
     3301 gatcggatag cgatccatca gcttggacag catgttgctc atgaacacca cgaaggggat
     3361 ggagagcccc agcccgaaca ggaggagtaa catactgcca tggctcgcgg ccccgacggc
     3421 cagcatgttg tcgatcccca tgctcagatc cgccacgaca atgatccaga gggcctgcca
     3481 gagaccggcg gcttcccgct gggcgtgggt tcgctcggtc gcgccctggg tgaggagctt
     3541 caccgcgatc cagaggatca cggcgccacc gatcagcttg acgtacggca tgaccaggag
     3601 ctgggccacc aggaaggtgc tgagcacccg gatgatgacc gccccaccgg agcccagcgc
     3661 gatcccccag aggcgctggc gcgccgacag gttcttgacg gccatggcga tgaccacggc
     3721 attgtcgccc gccaggacga ggtcgatgag cacgatgctc atgatcgccg agaagaatgc
     3781 ccacgtgaat tcgatctgcc caagccagcc caggtcaatc atggctcctc cgtgttccca
     3841 gatatgtatg gggcgccggc acgccgccgg acgcgatcat gtcaactcga gaaatcgatg
     3901 ggaccaagcc gccgcttggg gtgtcggcgg ggatagcgaa accggctggt ccaacgactc
     3961 cacgtgcccg cccgccgtct ggaaggaacg tctgcattct tcatggaggg tggcgcggca
     4021 cgccacggtc tcgactcccg cacatgagcc tgaaactcgg cgacgagctt ctccagctca
     4081 ccacggtcga gccacacacc tcgacactga cagcaggcat ccaccacgac accccctcga
     4141 gttcgttcgc tgagtaccac cccgcatcga ggacattgca
//
LOCUS       DBNGZC010000260         2718 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1488835, whole genome shotgun sequence.
ACCESSION   DBNGZC010000260 DBNGZC010000000
VERSION     DBNGZC010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1488835"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="AC16G0_04820"
     CDS             <1..616
                     /locus_tag="AC16G0_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5098981.1"
                     /translation="CFNLLTKFLTPTRGRIGFKGRDITSAKPAEIARLGLARSFQISA
                     VFPHLSVLENVRLALQRQRGNSFDFWRSQKVLERFNGRAMELIEAVGLDPFADAVAVE
                     LPYGRKRALEIATTLALDPEMMLLDEPTAGMGHEDIDRITTLIKQVSAARTVLMVEHN
                     LSVVADLCDAITVLVRGEVLAEGDYASVSRNPEVIQAYLGTAHA"
     gene            609..1328
                     /locus_tag="AC16G0_04825"
     CDS             609..1328
                     /locus_tag="AC16G0_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5098982.1"
                     /translation="MPDATAPMPLLTVMNLQAWYGESHILHGVDFEVHSGEVVTLLGR
                     NGAGKSTTIKSIMGMVGRRSGSIRLSGRELIGLPPNRIARLGIALCPEDRGIFSSLNV
                     EENLLLPPQVCPGGLSVEAIFGLFPNLRERLRSQGTKLSGGEQQMLAIARILRTGARL
                     LLLDEPTEGLAPLIVKHIGDTIGGLRAEGFTILLVEQNLHFGSTVADRHYIMEHGRVV
                     DMIPNAQLERSLDKLHEYLGV"
     gene            1374..2585
                     /locus_tag="AC16G0_04830"
     CDS             1374..2585
                     /locus_tag="AC16G0_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5098983.1"
                     /translation="MAQRHLIPLVAVLALLAGTAHAQISDNVVKIGVLNDQSGPYSDL
                     AGPGSVVAARMAVEDFGAAKKGMKVEILVGDHQNKPDVGSQIARQWYDVDKVDMIVDV
                     PTSSVVLAVNQVAREKNKALIVSTGATSDLTGKACTPNEIHWTYDTWALANGTGSAIV
                     KTGGDTWFFLTADYAFGHALERDTEAVVLKNGGRVVGRVRHPFPTSDFSSFLLQAQAS
                     KAKIIGLANAGTDTTNAIKQGAEFGIVKGGQQFAGLLVFLTDVHALGLEKAQGLLLTE
                     SYYWDLNDGTRAWSKRFAERHHQAMPTMAQAGVYSAVLHYLKAVEALKSDDGPKVVAK
                     MKELPTDDPLFGKGMIRADGRKIHPMYLFEVKKPSDSKGPWDYYKLRATIPAEQAFRP
                     IDHGDCPLVKK"
     gene            2605..>2718
                     /locus_tag="AC16G0_04835"
     CDS             2605..>2718
                     /locus_tag="AC16G0_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5098984.1"
                     /translation="MLGIPTQALFGQLLIGLINGSFYAMLSLGLAVIFGLLN"
BASE COUNT          494 a          865 c          869 g          490 t
ORIGIN      
        1 ctgtttcaac ctgctcacca agtttctgac cccgacgcga ggtcgcatcg gcttcaaggg
       61 ccgcgatatc accagcgcca aacccgccga gatcgcgcgt cttggtctcg cccgctcttt
      121 ccaaatctcc gcggtctttc cgcatctcag cgtgctggag aacgtgcgcc ttgcgctgca
      181 acgccagcgc ggcaactcct tcgatttctg gcgctcgcag aaagttctgg agcgcttcaa
      241 cgggcgggcg atggaattga tcgaagccgt gggcctggac ccttttgcag acgccgtggc
      301 ggtggagctc ccctacggcc gcaagcgggc cctggagatc gcgaccacgc tcgccctcga
      361 ccccgaaatg atgctcctgg atgagccgac cgccggcatg ggtcatgagg atatcgaccg
      421 catcacgacc ctcatcaagc aagtgtccgc ggctcgcacc gtgctcatgg tcgagcacaa
      481 cctgagcgtc gtcgccgatc tctgcgacgc catcaccgtg ctggttcgtg gcgaagttct
      541 tgcggaaggc gattatgcca gcgtgtcgcg gaatcccgaa gtcatccagg cctatctggg
      601 gactgcccat gcctgacgcc accgccccca tgccgctgct cacggtcatg aacctgcaag
      661 cgtggtacgg ggagtcccac atcctccacg gcgtcgactt cgaggtccat tcaggtgagg
      721 tggtgacgct cctggggcgg aatggggctg gcaagagcac cacgatcaaa tccatcatgg
      781 gaatggtcgg gcggcgctcg ggctcgatcc gattgagcgg ccgggagctg atcggccttc
      841 ctccgaaccg catcgcgcgg ctagggatcg ccctctgtcc tgaagaccgg ggaatttttt
      901 ccagtctgaa cgtggaggag aatctgcttc ttccgcccca ggtctgtccc ggtggcctgt
      961 cggtcgaggc gatcttcggc ctctttccca acctgcggga gcgcctgcgg agccagggca
     1021 ccaagctgtc gggcggcgaa cagcagatgc tggccatcgc ccggatcctc cgcaccgggg
     1081 cacggctgct cttgctggac gaacccaccg agggtctggc ccccttgatc gtgaagcaca
     1141 tcggcgacac catcggtggg ctcagggcgg aggggttcac cattctcctg gtcgagcaga
     1201 atctccactt cgggtccacg gtcgccgatc gtcactacat catggagcac ggtcgggtgg
     1261 tggacatgat cccgaacgcc caactcgagc ggagcctgga caagctgcac gagtatctcg
     1321 gagtgtaggt ccgcggggag acacctcaca catccacgag gggagggacg aacatggcgc
     1381 agagacacct cattccgctg gtggcggttc tggcgcttct ggccgggacg gcccacgctc
     1441 agatctccga caacgtggtc aagatcggcg tcctcaacga tcagtcgggt ccgtatagcg
     1501 acctcgccgg tcccggctcg gtggtcgccg cccgcatggc tgtggaggat tttggcgcgg
     1561 ccaagaaggg gatgaaggtc gagattctgg tcggggatca ccagaacaag cccgatgtcg
     1621 gctcccagat cgcccggcag tggtatgacg tcgacaaggt ggacatgatc gtggacgtgc
     1681 cgacctcctc ggtcgtcctg gccgtaaacc aggttgcccg ggagaagaac aaggcgctca
     1741 tcgtgtccac cggcgccacg tcggatttga ccggcaaggc ctgcacgccc aacgagatcc
     1801 actggaccta cgacacctgg gcactggcca acggcaccgg atcggcgatc gtgaagacgg
     1861 ggggcgacac ctggttcttc ctcacggcgg attacgcctt cggccacgcg ctggagcggg
     1921 acaccgaggc ggtggtcctg aagaatggcg gcagggtggt cgggcgcgtc cgtcatccgt
     1981 tcccgacgtc ggacttctcg tcgttcctgc tgcaggcgca ggcctccaag gccaagatca
     2041 ttggcctcgc caacgccggg acggatacca cgaatgcgat caagcaggga gcggagttcg
     2101 gcatcgtcaa gggcggccag caattcgccg gattgctcgt gttcctcacc gacgtgcacg
     2161 ccctcggcct ggagaaggcg cagggcctgc tgctcaccga gtcctattac tgggacctga
     2221 acgatgggac gcgcgcctgg tcgaagcgtt tcgccgagcg ccatcaccag gccatgccga
     2281 ccatggccca ggccggggtc tactctgcgg tcctgcatta cctcaaggcg gtggaggccc
     2341 tgaagagcga cgacgggccg aaggtcgtcg ccaagatgaa agagctgccg accgacgatc
     2401 cactcttcgg caaggggatg atccgggccg acggacgcaa gatccacccc atgtacctct
     2461 tcgaggtgaa gaagccgtcg gactcgaagg ggccctggga ttactacaag ctgcgggcga
     2521 cgatcccggc cgagcaggct ttcaggccga tcgatcatgg cgactgcccg ctggtcaaga
     2581 aataggctgc gagggaccca cgccatgttg gggattccca cacaggctct gttcggtcag
     2641 ctcctgatcg gactgatcaa cggctcgttc tacgcgatgt tgagtcttgg actggcggtg
     2701 atcttcggtc tgctcaac
//
LOCUS       DBNGZC010000261         1849 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1782664, whole genome shotgun sequence.
ACCESSION   DBNGZC010000261 DBNGZC010000000
VERSION     DBNGZC010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1782664"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="AC16G0_04840"
     CDS             <1..895
                     /locus_tag="AC16G0_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5098985.1"
                     /translation="MDRSVAFYSKVLSFEKVLDVEVWGDEYEHLQGVFGLRMRVVRMR
                     LGDETIELTQYLAPRGRPIPLDSRSNDRWFQHIAIIVSDMDRAYDWLREHKVQHASPA
                     PQRLPDWNTKAAGIRAFYFRDPDDHNLEILEFPPDKGDPRWHRPTGRLFLGIDHTAIV
                     VGDTEASLGFYRDVLGLRVVGESENYGPEQERLNNVFGARLRITTLRSPVGPAIEFLE
                     YLAPRDGRPLPADVHANDLVHWQTRLLTQDVVTTAGVLRTNRVRFVSPGVVSLSGTQL
                     GFTRGFLVRDPDGHGIELTEK"
     gene            903..>1849
                     /locus_tag="AC16G0_04845"
     CDS             903..>1849
                     /locus_tag="AC16G0_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016864374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HIR5098986.1"
                     /translation="MRIDMGREICGHLAVAESREWLCTNGIGGFASGTVAGLLSRRYH
                     GLLLAALKPPLGRTLLVAKLDETVEYDGLSRALYTNRWADGTVDPHGYREIERFRLDG
                     TTPVWTYAIADALLEKRVWMEQGANTTYVQFRLLRARAPVALELKTLVNYRDYHATTR
                     GHGWRMRVEPVPGGLRVVAFDGAQPFLLLADGAEAQPAHEWYQGVDLSAERERGLDSR
                     EDHLHAGTLRGTLAPGATLTCVLSSEAAPSLDGEAAWRRRKRHEEQLLDGWRNAHSST
                     GRAPAWIQHLVLAADQFVVRRPLPDDPGGMSAIAGYHWFG"
BASE COUNT          320 a          574 c          641 g          314 t
ORIGIN      
        1 gatggatcgg tcggttgcgt tctactcgaa ggtgctctcg ttcgagaaag tgttggacgt
       61 tgaagtgtgg ggggacgagt acgaacatct tcagggggtc ttcgggctga ggatgcgggt
      121 ggtgcggatg cgcctcggcg acgagaccat cgagttgacg cagtatctgg cgccgagagg
      181 acggcccatc ccattggatt cgcggagcaa cgatcgctgg ttccaacata ttgccatcat
      241 tgtcagtgac atggatcggg cgtatgactg gctgcgcgag cacaaggtac agcatgcgtc
      301 cccggccccg caacgcttgc cggattggaa cacaaaggcc gccgggatcc gggccttcta
      361 tttccgggat cccgacgacc ataacctcga gatcctcgag ttccccccgg acaagggtga
      421 tccaagatgg catcgcccca cggggagact gtttctcggc atcgaccaca ccgccatcgt
      481 ggtcggggac acggaggcaa gcctgggctt ctaccgggac gtcctggggc tccgggtggt
      541 gggggagagt gaaaattacg ggccggagca ggagcgtctc aacaacgtgt tcggtgcccg
      601 actcaggatc accacgctcc ggtcgccggt cgggcccgcc atcgaattcc tggagtacct
      661 tgcgccccga gacggacggc cgctgccggc ggacgtgcat gccaacgact tggtccactg
      721 gcagacgcga ctccttaccc aggacgtggt gaccacggct ggggttctcc ggacgaaccg
      781 ggtgagattc gtgtcgccgg gggtggtcag cttgagcggg acacagctcg ggttcaccag
      841 aggcttcctg gtccgagatc cggacggtca cgggatcgag ctgacggaga agtgaggctg
      901 aaatgcgcat cgacatgggt cgcgagatct gtggacacct ggcggtggcg gaatcccgag
      961 aatggctctg caccaacggt atcggcggct ttgcctcagg gacagtagca ggcctgctca
     1021 gccggcggta ccacggcctc ctgctggccg ccctgaagcc accgctcggg cgcacgttgc
     1081 tggttgccaa gctggatgag accgtcgaat acgatgggct gagccgcgcc ctctacacca
     1141 accgctgggc ggacggaacc gtcgatcccc acggctaccg cgagatcgag cggttccggc
     1201 tggacgggac gactcccgtc tggacctacg ccatcgccga tgccctgctg gagaagcgag
     1261 tctggatgga gcagggggcg aacacaacct acgttcaatt tcgcctgctc cgggcgcgtg
     1321 cgcccgttgc cctggaactg aagacactgg tgaactaccg ggattaccac gcgaccacac
     1381 gagggcacgg atggcggatg cgggtggagc cggtgcctgg cggcctgcgg gtcgtggcat
     1441 tcgacggggc acagccgttc ctcctgctgg ccgacggggc ggaggctcag cccgcccatg
     1501 agtggtacca gggcgttgac ctgtccgccg aacgcgagcg agggctcgac tcccgtgaag
     1561 atcatctgca cgcggggact ctccggggaa cccttgcgcc gggtgcgacg ttgacctgcg
     1621 tcctctcgtc cgaggccgcg ccgtccctag acggagaggc cgcctggcgg cggcgaaagc
     1681 ggcacgagga gcaactgctg gatggctggc ggaatgccca ttccagcacc ggccgggcgc
     1741 ctgcctggat ccagcacctc gtcctggccg cggaccagtt cgtcgtccgt cgcccgctcc
     1801 ccgacgatcc gggggggatg tcggccatcg ccggctacca ctggttcgg
//
LOCUS       DBNGZC010000262         3220 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1977564, whole genome shotgun sequence.
ACCESSION   DBNGZC010000262 DBNGZC010000000
VERSION     DBNGZC010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1977564"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="AC16G0_04850"
     CDS             complement(<1..928)
                     /locus_tag="AC16G0_04850"
                     /inference="COORDINATES: protein motif:HMM:NF052759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR5098987.1"
                     /translation="MRRGKLGLISALIILVLGGAGGFYWLGTGGRQASYRTVPVEQGN
                     IQSTISSTGTLNAVITVQVGTQVSGTIQQLFVDYNSPVKKGMLIARIDPATFTAKVNQ
                     AQADVESAQATVLNQRAAVAKAQADVANAKANVVKDEVAVRDAKIKADSRIRLFQEGG
                     ISEEERDTAKATLDSAVAQLDAAQASVRSSQAALEVTQAQLAAAQAQVRQKQAALAQA
                     QVDLDNSNIRAPVDGVVVARNVDVGQTVAASLQAPTLFLIAQDLTKMQVDTNVDEADI
                     GRVAMEQVAAFTVDAYPGQRFEGKVVQVRQAPQ"
     gene            complement(1166..1921)
                     /gene="phnE"
                     /locus_tag="AC16G0_04855"
     CDS             complement(1166..1921)
                     /gene="phnE"
                     /locus_tag="AC16G0_04855"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HIR5098988.1"
                     /translation="MTGRWKRAGCWLAVLAVYAWFLGATEVSFPRFLRGLPWMADFVR
                     RMVPPDLSVLGSAVTGALQTLQIAVVGTTVAAVLALPLGFLSARNVVAASSFYPARCL
                     LNFFRSIDTMVYALFFVAAVGLGPFPGVLAVVTYTTMTLAKLYSEAIEGIEPGAVEAI
                     TATGASRLQVLRFGILPQVLPLFASYVLYRLESNIRAATILGFVGAGGIGFYIQTYLR
                     MINYPAAAAVLLVLVAMVMGVDFASSRLRAHLV"
     gene            complement(1918..2661)
                     /gene="phnC"
                     /locus_tag="AC16G0_04860"
     CDS             complement(1918..2661)
                     /gene="phnC"
                     /locus_tag="AC16G0_04860"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018111221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HIR5098989.1"
                     /translation="MIHIRGLRKVFPPDKVALDGVDLHVARGEFVMVIGQSGAGKTTL
                     LRCLNRLVEPTAGEVRLNGSDVTGSSPEALREVRRQIGMIFQQFNLVRRASVLENVLT
                     GRLGYVSPLPSLVGRFPGTDRDLAMDCLRLVGLEELADRRADTLSGGEQQRVAIARAL
                     AQRPKVILADEPTASLDPKLTESIMGILQRINVERGLTLMVSQHMLETALAFGTRIVG
                     LRQGRVAFDGPPSRITPDLVADIYEGGVR"
     gene            complement(2726..>3220)
                     /locus_tag="AC16G0_04865"
     CDS             complement(2726..>3220)
                     /locus_tag="AC16G0_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR5098990.1"
                     /translation="KSVEELRGKTIAFADPASTSGYVYPMVLLMKKGLVKNRDPKTFF
                     KEAMFAGTHEAALLALLNGSVDAVASFDLAPEQYLKDKERVEKLTFIAETDPIPEAGM
                     VVREGLDPALVKRLQDALMAFNAPEFRPILKDFYGIDGFAPAKDSDYNSVRDAIDILG
                     WRPK"
BASE COUNT          576 a         1105 c          973 g          566 t
ORIGIN      
        1 cctggggggc ctggcggacc tgcaccacct tgccctcgaa gcgctgcccg ggatacgcgt
       61 ccacggtgaa ggccgcgacc tgctccatgg ccacccggcc gatgtccgcc tcatccacat
      121 tggtgtccac ctgcatcttg gtgagatcct gggcaatcag aaagagggtc ggggcctgga
      181 gggaggccgc cacggtctgc cccacgtcca cgttccgtgc gaccactacc ccgtccacag
      241 gcgcccggat gttggaattg tccagatcca cctgcgcctg agcgagggcg gcctgtttct
      301 gcctcacctg ggcctgggca gcagcgagtt gtgcctgcgt gacttccagg gctgcctgag
      361 aggagcggac gctcgcctgc gctgcatcca actgggccac ggccgaatcc agggtcgcct
      421 tggccgtatc ccgctcctcc tccgagatcc cgccctcttg gaagaggcga attcgcgagt
      481 cggccttgat cttggcgtct cggaccgcca cttcatcctt caccacgttc gccttggcgt
      541 tcgccacatc cgcctgggcc ttggccaccg ctgcccgctg gttcaggacc gtcgcctggg
      601 cgctctccac atccgcctgg gcctgattga ccttggcggt aaaggtggcc gggtcgatcc
      661 gcgcaatcag catccccttc ttcaccggcg aattgtagtc cacgaagagc tgctgaatcg
      721 tgcccgagac ctgggttccc acctggacgg tgatgaccgc attcagcgtc ccggtggacg
      781 agatcgtgga ctggatgttg ccctgttcca ccgggacagt tcggtaggaa gcctgccggc
      841 ccccagtccc cagccagtag aatccgccgg ctccgcccaa gaccaggatg atcagtgccg
      901 aaatcagccc cagcttcccg cgcctcatgt cccgctggcc ctctcgctca tattcctggt
      961 gtctcgatcc ggtccaacgc ccataaccgg cccaatgccc ataatacgac ctggcgtgcc
     1021 atggcgtttc acatgagtgt acttgccctt accggggctg tcaaaaccta tcggctttcg
     1081 gcagcctacc gtgttcgatt cgtgaaaaac cgccccatcg tctgccttcg ccccgacaat
     1141 cccgtcgtcg cgcagggacc tccccctaga ccaggtgtgc tcgcagccgg gagctggcaa
     1201 agtccactcc catgaccatg gcgaccagca ccagcagcac ggctgcggcc gccggataat
     1261 tgatcatccg gagataggtc tggatgtaga agccgatgcc ccccgccccc acgaatccca
     1321 agatcgtggc cgcccggatg ttcgattcga gccggtacag cacgtaggag gcgaagagtg
     1381 ggagcacctg tggcaggatg ccgaagcgga gaacttgcag ccggctcgcc ccggtcgcag
     1441 tgatggcttc gaccgctcca ggctcgatcc cctcgatggc ctcggagtaa agcttggcca
     1501 gggtcatggt tgtgtaggtg acgaccgcca ggacacccgg aaacggcccc agccccaccg
     1561 ccgcgacaaa aaacagagcg tataccatgg tgtcgatgct gcggaagaag ttgagaaggc
     1621 atcgcgcggg atagaagctc gacgctgcca ccacgttccg ggccgagaga aagcccagag
     1681 gcagggccag caccgcggcg accgtggttc ccacgacggc gatctgcagc gtctggagcg
     1741 ccccggtgac cgcgctcccc agaaccgaga gatccggcgg gaccatacgg cgaacgaagt
     1801 cagccatcca gggaagaccc cggaggaatc gcgggaagct gacttccgtg gcgccgagga
     1861 accaggcgta gaccgccagg acagcgagcc agcaccccgc gcgtttccac ctgcccgtca
     1921 ccgcactcct ccttcgtaga tgtctgcgac caggtctggc gtgatccggc tgggcggccc
     1981 gtcgaatgcc acccgcccct ggcgtaaacc gacgatccgc gtcccgaagg ccaaggccgt
     2041 ctccagcatg tgctggctca ccatgagggt cagacctcgc tccacattga tcctctgcag
     2101 gatacccatg atgctctcgg tgagcttggg gtccaggctg gcggtgggtt cgtctgccag
     2161 gatgaccttg ggtctctggg ccagggcccg ggcgatggcc acccgctgct gttctccgcc
     2221 ggacagcgtg tccgcgcgcc gatccgcaag ttcctccaga cccaccaggc gcaggcagtc
     2281 catggccaga tcgcgatctg ttccggggaa ccggccgacg aggctcggaa gcggggagac
     2341 gtacccgagt cttcccgtga ggacgttctc caagacgctg gctcgccgca ccaggttgaa
     2401 ctgctggaag atcatcccga tctgacgccg gacctcgcgg agtgcctccg gcgaggaccc
     2461 cgtcacgtcc gacccgttga gccgcacctc acccgccgtt ggctccacca gccggttcag
     2521 gcagcgaagg agcgtggtct tgcccgcacc actctgcccg atgaccatga caaactcgcc
     2581 acgggccacg tgcaggtcca ccccgtccag ggcgacctta tctggcggga ataccttgcg
     2641 aagaccgcga atgtggatca tacgctctgc ggcatcatgc ggtagcgggt gcccccgcgc
     2701 gtgaaagcga cggctggcac ggtcactatt tcggccgcca gcccagaatg tcgatggcat
     2761 cccgcacgga gttgtagtcg ctgtctttgg ctggcgcgaa tccgtcgatg ccgtagaagt
     2821 ccttgaggat gggccgaaac tctggagcgt tgaaggccat gagggcatcc tggagcctct
     2881 tcacgagagc gggatccaga ccttcccgga cgaccattcc cgcctccgga atcggatcgg
     2941 tctcggcgat aaaggtcagc ttttcaaccc gctccttgtc cttcagatat tgctccggcg
     3001 ccaggtcgaa ggaggcgacc gcgtccacac tgccgttcag cagggccaag agggccgcct
     3061 cgtgggtccc ggcaaacatc gcctccttga agaaggtctt gggatcgcgg ttcttcacca
     3121 accccttctt catcaacagg accatgggat acacataccc ggacgtggag gccggatcgg
     3181 cgaaggcaat ggtcttcccc cgcagctcct ccacggactt
//
LOCUS       DBNGZC010000263         5314 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_510267, whole genome shotgun sequence.
ACCESSION   DBNGZC010000263 DBNGZC010000000
VERSION     DBNGZC010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_510267"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..1794
                     /locus_tag="AC16G0_04870"
                     /pseudo
     CDS             217..1794
                     /locus_tag="AC16G0_04870"
                     /inference="COORDINATES: protein motif:HMM:NF039835.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
     gene            1810..3102
                     /locus_tag="AC16G0_04875"
     CDS             1810..3102
                     /locus_tag="AC16G0_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012271.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR5098991.1"
                     /translation="MTVDTTKSVTATFSSVDTTPPPPPINLAANGAVGQSAWTRSNGF
                     GITWTNPSDPSGIVRAFYKLGSVPTSPTDGIPASATGFNLTATSEAGQPLYIWLEDGA
                     GNKDSANRGGVTLRYDATPPVTRSDAPTNWLGTDRTMTVYLTATDAGGSGVASTQYCT
                     DTTNVCTPSTVGTSVSVGCPAGTACQTYIRFRSTDIAGNVEAIQSAIVRIDSAPPAAV
                     TNLAANTATQTTVPLTWSTPSDSGSGNASFDLRYRLGTTFSDSDWPAGTRVTGVPIPP
                     ATGITVSGLVCGTTYTFAIRTLDRVGNISPLSNTATQTTVACSNTPPGRPVNSNPTDR
                     ASGVALSPTLQASAFSDLDPGDTQAASQWQIFNSRGTLIWDSGEDSTNTISRTVPTNA
                     GLRSNTTYRWQVRYRDNRGAWSSYSARTSFTTQRRNRW"
     gene            complement(3790..3912)
                     /locus_tag="AC16G0_04880"
     CDS             complement(3790..3912)
                     /locus_tag="AC16G0_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098992.1"
                     /translation="MNIQELNEEGVLPSCQFGAFSGVGCPLDLLMHGRCHRNAR"
     gene            4250..4477
                     /locus_tag="AC16G0_04885"
     CDS             4250..4477
                     /locus_tag="AC16G0_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5098993.1"
                     /translation="MVGEAGDSQEAPEHAQALMPDISLAGIYMPSMSGLEVTRRIMEV
                     RKLVSQGKNIKDIGAALAIAGSSIKSYLKIF"
     gene            4835..>5314
                     /locus_tag="AC16G0_04890"
     CDS             4835..>5314
                     /locus_tag="AC16G0_04890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR5098994.1"
                     /translation="MVDSRDPEAPVLFLTGDVPMGPRPASGPRGPNLERWKWLGVWLP
                     IIALGSGIILLEGLLAALALPRGFTLMGHGLILALAAVGAEFLAEHTFRAVRGMGEEI
                     LQRQQELALLERRFKALIEDSSDGIVLLDARGTFLYASPSTARLLGCPVEELVGGNAF
                     "
BASE COUNT         1019 a         1808 c         1611 g          876 t
ORIGIN      
        1 acatacgccc tgctcaacac cgtgggggct gtcttcacct acaccgaccc tggcctcccc
       61 gtgggggcca cctactgcta ccaggtccgg gcctacaatg cggccgggga ctcccccccc
      121 tccaacgagg cctgcgggac ggtggcggcc ccggtgctca ccaccctcag cgtgaccaaa
      181 gctgggaccg gctccggggc tgtgaccagt agccccgtgg ggatcgactg cggcgcgacc
      241 tgcagcgcca gcttcacctc tgggacgagc gtcaccgtca ccgcgacccc ggcgacgggc
      301 tccaccttca ccggctggag cggagcctgc accgggaccg gaacgtgttc cgtgaccgtg
      361 gacaccgcca agagcgtcac ggccaccttt acgttgcagg ccttccctct cacggtcacg
      421 aaggcgggag ccggcaccgg gagcgtcacc tcggccccgg ctggcatcac ctgcgggagt
      481 acctgcaacg ccagcttcac gtccgggacg agcgtcaccc tcaccgcgac ccagtcgacg
      541 ggctccacct tcaccggctg gagcggggcc tgcacgggga ctggggcctg cacggtgacg
      601 atggctgctg ccaagaatgt gacggccacc ttcacgctgc agaacttgac gctcactgtg
      661 gcgaagagcg gcaccgggac gggcagcgtg accagcagcc ccgcggggat cagctgcggg
      721 agtacctgca gcgccagctt cacctccggg acgaacgtca ccctcactgc gactccggcc
      781 acgggctcca ccttccccgg ctggagcggg gcctgcaccg ggaccgggac ctgcatggtg
      841 acgatggctg ctgccaagac cgtcacggcc accttcgcac tgcagaactt cacgctcact
      901 gtgacgaaga gcggcaccgg gacgggcagc gtgaccagca gccccgcggg aatcgcttgc
      961 gggagcacgt gcggcgccgc ctttgcctct ggtgccagcg tcactctcac cgcgaccccg
     1021 gcgacaggtt ccagcttcac cggctggagc ggggcctgca ccgggaccgg gacctgcaca
     1081 gtctcgatga ctaaggcgca gagcgtcacg gccaccttcg ccctgcagac ctccagtctc
     1141 agcgtgacta aagctgggac cggctccggg gcggtgatca gtagccccgt ggggatcgac
     1201 tgcggcgcga cctgcagcgc cagcttcaac tccgggacga acgtcaccct caccgccacg
     1261 cccgccagca cttccgcctt tacgggttgg agcggggcct gcaccgggac cggaacgtgt
     1321 tccgtgactg tggacaccgc caagagcgtg acggccacct ttacgctgca ggcctccacc
     1381 ctcagcgtga ccaaggccgg cacggggacg ggcaacgtga gcagtagccc tggggccatc
     1441 gactgcggcg cgacctgcag cgccagcttc aactccggga cgaacgtcac cctcaccgcc
     1501 acgcccgcca gcacttccac ctttacgggt tggagcgggg cctgcaccgg gaccggaacg
     1561 tgttccgtga ctgtggacac cgccaagagc gtgacggcca cttttacgct gcaggcctcc
     1621 accctcacgg tcacgaaggc gggagccggc agcgggagcg tcacctcgac cccggctggc
     1681 atcacctgcg ggagtacctg caacgccagc ttcacctctg ggacgagcgt caccctcacc
     1741 gcgacctcgg cgacgggcgc caccttcacc ggctggagcg gggcctgcac cgggaccggg
     1801 acgtgttccg tgaccgtgga caccaccaag agcgtcacgg ccaccttcag ctccgtcgac
     1861 actacaccgc cgccgcctcc catcaacctc gcggcgaatg gcgcggtagg tcagagcgcc
     1921 tggacaagat ccaacggatt cggcatcacc tggaccaacc ccagtgatcc ctcgggcatc
     1981 gtccgggcct tctacaagct cgggagcgtt ccgacaagcc cgacggatgg catcccagcg
     2041 agtgccaccg ggtttaactt gacggcgaca tccgaggccg gccaaccctt gtacatctgg
     2101 ctggaggacg gcgccgggaa caaggactcc gcgaaccggg gcggtgtcac tctacgctac
     2161 gacgccacgc ccccggtcac ccggagcgat gcccccacca actggctcgg gaccgatcgg
     2221 accatgacgg tttacctcac ggcgacagat gctggtgggt ccggagtggc cagcactcag
     2281 tactgcacgg atacgaccaa tgtttgtacg ccatcgacgg tcgggacctc ggtctcggtg
     2341 ggctgcccgg ccggcacggc ctgtcagact tacatccggt tccgttccac ggatatcgcg
     2401 gggaatgtcg aggcgatcca gagcgccatc gtgagaatcg actcggcccc ccctgctgcc
     2461 gtgaccaacc tggcggctaa cacggcaacc caaacaacgg tgccactgac gtggagcacc
     2521 ccatcggaca gcggttcagg aaatgccagc ttcgaccttc gataccggct cggcacgacc
     2581 ttcagtgatt ctgattggcc ggcgggcacc cgagtgacag gagtacccat cccacccgcc
     2641 accgggatca cggtctcggg ccttgtctgc gggaccacct acacctttgc catccgaacc
     2701 ctggatcggg tcggaaacat ctcacccctc tcgaatactg ccacacaaac caccgtggcc
     2761 tgttccaaca caccacccgg tcggcccgtc aacagcaatc caaccgaccg ggcatcaggt
     2821 gtggctctct cgccaaccct ccaggcctcg gccttcagcg atctcgatcc gggggacacg
     2881 caggccgcca gccagtggca gatcttcaat agccgcggca ctctcatctg ggacagcggg
     2941 gaagattcca ccaatacgat tagtcggacg gtcccgacca acgccggcct gcggtccaac
     3001 acgacctacc gttggcaggt gcgctatagg gataatcgcg gtgcatggag ttcatactca
     3061 gcccggacca gcttcacgac tcaacgcagg aatcgctggt gagccggcag tagcttgagt
     3121 catatactcg gatcgagtcc ctttcctcgg tttaacattc gcgagaggag aagcacccac
     3181 tcataggcca ctcctgaact cacgttcaac tcctccgccg acaggagaga ccggcagatt
     3241 ccgtgcgctc acgccgtcgc agacccctgt gctctggaaa tacactcgcc gctctcccgt
     3301 cgcgaaacgc tccagccaat atttggaatg caagccaccg agatccggtc gcgccaccac
     3361 acgggatagc aacggaaggt gatgtccaac agtcggacgc accgacagaa agggaggtgg
     3421 cccagccgtc ggatccccta tccggtattt gagccaccga catgagggtt atgcaacttc
     3481 cgccctgagt tgggaccacc taggacacgc gcattcggga ttgaccggct aggagcgcct
     3541 ggctctgata agagctcggg tgacccagcg gcgggagatg ccgcgaaaac tagaagccat
     3601 caagacggtg gatgagagtc ggcgatctga ggataccctg cgaccctgac catgcgtgcg
     3661 tcggttggag ttgacacaaa cattttggcg ttcctcgctc aggccgcaag gggcatgtac
     3721 gaccctgcag ccgatgcgca tagagtcctc aggcctgagc gggtcaccgc ctatcgggtt
     3781 ttccttcact cagcgtgcat tgcggtgaca ccgaccgtgc atgaggagat ccagcggaca
     3841 gccgacccca gagaacgcgc cgaactggca ggatggaagg acgccctcct cgttgagctc
     3901 ctggatgttc atcccgatgc ggtcgaggcg aggacccgat ggctccgaag gcaccacacc
     3961 tgggagcggg attgccggat tgttgcggag gcggagcttg ccggattgga tgtgctcctc
     4021 accttcgaca aaacgctccg gaagggccta gatggtcgta ccgcccccct gcgcatcaga
     4081 tacccctccg aatactgggc ggaactccag attccgcgtg ggcagatgag ccttcagtct
     4141 ccagttccaa cgaagccact tcacactgcc tcttggtggc gctgggagtg aagcgctaca
     4201 cacccccgcc ctgtttctac cgggagagtc tgaagggaga agatcagagg tggtcgggga
     4261 ggcgggcgat agtcaggagg ccccggagca cgctcaggct ctcatgcccg acatcagtct
     4321 cgcgggcatc tacatgccta gcatgagcgg cttggaggtc acacgccgga ttatggaggt
     4381 tcggaagttg gtcagccaag gcaagaacat caaggacatt ggcgctgccc tggccatcgc
     4441 tgggagcagc atcaagagct acctcaagat attctaggca gggtcgcacc cggagaaccg
     4501 ggtataggcg gccacattcg ccttccacaa gggctggctg gagagtcctc gggataggtc
     4561 gactaccttc tcctcctcga actcccctca cttccctcac tgccctctgt ctctcggttc
     4621 gcagcgggca cctcgcgtac ccccagtaag aggggtgtcc gaccccccaa tggagggggg
     4681 tcactcccct tccttttctc cagaaatcgt accagagagg gactagattg aagcggcaaa
     4741 tacggcgata ttaccacggg ccggcttcat tcatctcgaa cgcttcacgc acacttctga
     4801 tagacaagaa acgcatccaa agaaggaaag tcaaatggtc gacagtcgcg atcctgaagc
     4861 tccggtgctg tttctcacgg gagatgtgcc gatgggcccc cggcccgcaa gtgggccgcg
     4921 agggccgaac ctggaaaggt ggaaatggct cggggtatgg ctgcccatta ttgccctggg
     4981 ttctgggatc atcctgctgg aaggcctcct ggctgcctta gcccttcccc gggggttcac
     5041 cctgatgggt cacgggctca ttctggcgct ggcggccgtc ggggcagagt tcctggcaga
     5101 gcacaccttc cgcgccgtcc gcgggatggg agaggaaatc ctccaaagac agcaggagct
     5161 ggcccttttg gaacgacggt tcaaggccct gatcgaagac agctcggacg ggattgtcct
     5221 gctcgatgcc cggggcacct tcctgtacgc cagcccctcc accgctcgac ttctcggatg
     5281 ccccgtggag gaattggtcg gcggcaacgc cttt
//
LOCUS       DBNGZC010000264         1569 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1977684, whole genome shotgun sequence.
ACCESSION   DBNGZC010000264 DBNGZC010000000
VERSION     DBNGZC010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1977684"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /gene="vorB"
                     /locus_tag="AC16G0_04895"
     CDS             complement(<1..993)
                     /gene="vorB"
                     /locus_tag="AC16G0_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HIR5098995.1"
                     /translation="MAKKLMKGCEAIGEAAILAGCRLFFGYPITPQNEVPEYMAARLP
                     QVGGTFLQAESEVAASNMLYGAAGAGCRCFTSSSSPGISLMMEGISYMAGAELPVVVV
                     NIMRGGPGLGGILPSQSDYFQAVKGGGHGDYRCLVLAPSTVQEAADLVFEAFDLADRY
                     RNPVMVLGDGLIGQMMEPVEFKHSIDPEDLPPKPWATTGAKGRKPNTINSLHLDPEKL
                     EAHNWTLAAKYQAMARTECRHEAWNLDGEVDVLFVAYGTVARICKTAIELLKQRGVRG
                     GLFRPVTLFPYPGAAWAKAAERSGHVLVSELSCGQMVEDARYHVAGRRPVDFYGR"
     gene            complement(1004..>1569)
                     /locus_tag="AC16G0_04900"
     CDS             complement(1004..>1569)
                     /locus_tag="AC16G0_04900"
                     /inference="COORDINATES: protein motif:HMM:NF013707.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR5098996.1"
                     /translation="IMETEIVLAGFGGQGVLLIGKLLAYAAMKAGREVTWLPAYGPEM
                     RGGTCNCTVVLSDRPIGSPISKSPHGLIVLNLPSLDKFEETVRPGGIIVVNTSLINRL
                     PRRTDVIVVAVPANEVALECGSAKAANMVTLGAYLGASGIAELAQVKAIITESFAAKP
                     ALVTINHEALSRGFALGKEQAASVALH"
BASE COUNT          275 a          521 c          504 g          269 t
ORIGIN      
        1 ccggccgtag aagtccacag ggcgtcttcc cgccacgtgg taccgggcgt cctccaccat
       61 ttgtccacag gacagctcgg aaaccaggac atgtcccgac cgctctgcgg ccttggccca
      121 ggccgccccc gggtagggga agagcgtgac gggtcggaat agtccccccc tcactccccg
      181 ctgcttgagg agctcgatgg cggtcttgca gattcgcgcc acggtgccgt aggccacgaa
      241 gaggacatcc acctcgccat cgaggttcca cgcctcatgg cggcattcgg tgcgtgccat
      301 ggcctgatac ttcgcggcga gcgtccagtt atgcgcctcc agcttctcgg ggtccaggtg
      361 aagggagttg atggtgttgg gcttcctccc tttggcgccg gtggtggccc agggcttggg
      421 cggcaaatcc tcgggatcga tcgaatgttt gaactccacc ggctccatca tctggccgat
      481 gagaccgtcg cccagaacca tgacggggtt gcggtatcga tccgccaggt caaacgcctc
      541 aaacaccagg tccgccgcct cctggacggt ggaaggagcc aggaccagac agcggtagtc
      601 gccgtgaccg ccacccttca ccgcctggaa gtagtccgac tgggacggga ggatcccgcc
      661 caatccgggt ccaccccgca tgatgttgac aaccaccacg gggagttcgg cgcccgccat
      721 gtacgagatc ccttccatca tcaggctgat gccggggctg gaggatgagg tgaagcagcg
      781 acagcccgcg cccgcggccc cgtacagcat gttgctggcc gccacctcgc tctcggcctg
      841 gaggaaggtc ccacccacct ggggcaggcg ggcggccatg tactcgggga cctcgttctg
      901 gggcgtgatg gggtagccga agaagaggcg acagcccgcc aggatcgcgg cctcgccgat
      961 cgcctcacag cccttcatca acttcttggc catcgagtgc tccttagtgc agggcgacgg
     1021 acgcggcctg ctctttaccc agggcaaagc cacgagacag cgcttcatgg ttgatcgtca
     1081 ccagcgcggg cttggcggcg aaggactcgg tgataatggc cttgacctgt gccagctcgg
     1141 cgatgccgct ggcccccaga taggcgccca gcgtcaccat gttggcggcc ttggcgctgc
     1201 cgcactcgag ggccacctcg ttggcgggca cagccaccac gatcacgtcc gtccgccgcg
     1261 gaaggcggtt gatgagggag gtattgacta cgatgatgcc ccccggccgc accgtctcct
     1321 cgaacttgtc cagcgagggc aggtttagga cgatgagccc atggggagac ttgctgatcg
     1381 gggacccgat gggccggtcg gagaggacga ccgtgcagtt gcaggtgccg ccccgcatct
     1441 caggaccgta cgccggtaac cacgtgacct cacgacccgc cttcatggcc gcataggcaa
     1501 ggagcttccc gatgagcaga accccctggc caccgaatcc ggcgaggacg atctcggttt
     1561 ccatgatta
//
LOCUS       DBNGZC010000265         2709 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1488989, whole genome shotgun sequence.
ACCESSION   DBNGZC010000265 DBNGZC010000000
VERSION     DBNGZC010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1488989"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..1355
                     /locus_tag="AC16G0_04905"
                     /pseudo
     CDS             3..1355
                     /locus_tag="AC16G0_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_068413152.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            1507..2523
                     /locus_tag="AC16G0_04910"
     CDS             1507..2523
                     /locus_tag="AC16G0_04910"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HIR5098997.1"
                     /translation="MEPRDVLPADGLEWRRFRALQLRREGWSRQDVAAALGVAPASVS
                     RWFARARDDGPEALRARPRPGRPPKLSAAQKRLIPEFLWHGTEAYGFRGEVWTCARVA
                     RVIEEEFGVHYHKDHVSRLLRELQWTPQVPIRRAIQRDEDAIRHWRDTVWPDLQRRAR
                     RERRVLIFEDESGFYLLPGVVRTYAPQGQTPVLRETRTRDHLSVMGGMTPEGKLYALA
                     RQESLNGLDTIEFLLHLGRVAGERLLVIWDGSPIHRRSEVNEFVSETRGKVWLEALPG
                     YAPDLNPWDEGGWHHLKDVEMRNLVCRDLEELHQEFHLAVGRLRQKPRLIRSFFAQAG
                     LKIE"
BASE COUNT          442 a          889 c          947 g          431 t
ORIGIN      
        1 gcctgcaatt cgaacccgaa ctcgcccagc tcgaccgcct cctcgacgac gatgcgatct
       61 tcgtccgcgt ccgcgccgac ctggcgaagc gctatcccct gacccccgtc catggccgcc
      121 gctccacccc cgtcgaagtc atcctgcgga tgctcgtcgt catgcggctc tacggctgga
      181 gctccgcgca gaccgaatac tttgtccacg acagcctggt cctccggcag ttctgccgcg
      241 tttacctgga gaaggtgccc gatgacaccg tgctgatccg ctgggccaat accatcgggc
      301 cggagaccgt gcaaaagctc aacgaccggg tcgtccagtt ggcccgcagc ctgaaggtca
      361 cgcgcggccg cacgctccgc gtcgacacca ccgccgtcga gaccaacatc cattatccga
      421 ttgatagcgc cctgttgggc gatggggtgc gggtgctctc gcgactgctg cgccgcgcca
      481 agacggtcct ggggaaagcg gcgaacgcgc tcggacccga ggcatttcgc agccgggtgc
      541 ggaccgtccg caaactcgcc cagcagttgc atcggatcgc acgccgcaag ggggccgaag
      601 cccgcgacgc catgaagaat gcctatgagc atttgatcca tacggcaaag cgtaccggca
      661 accaagcccg tcgcgtgctc gaagtccttc aacgccggac gaagcggcca gcccagcgtc
      721 tcgctcaacg gatcggcacg ttcttgcccc tgttgatcca aggcatccag caggcgtccc
      781 gccgggtcct ggagggccag gcggtcccgg cctccgagaa agtgctgtcg ctgttcgagc
      841 cgcacaccca gatcatcccc cggcacaagg ccggcacgcc ggtcgagttc ggccgcaaga
      901 tccggctcga cgaggtcgag ggcgggatca tcaccgggta tgcgatcctg gagcagggtg
      961 gcgggcagga ccagccgtac ctcaaggaca gcctggaggc ccatcgccgg cacttcggcc
     1021 gggcgccgcg cctactggcg gcggaccgag ggatgtcctc agccgagaat gaacggctgg
     1081 cgcgcgaggc cggagtgaag caagtggcgc tgccccacgt gggccaggcg tcggcggagc
     1141 ggaaggcgcg ggaacggggt cggccgttcc ggcgggggta gcggttccga gcggggatcg
     1201 aggggcggat ccatgtgctg cggcgggacc ggggcctgaa gaggtgccgt taccagggtg
     1261 agggtgggag gggtcggtgg gtcggttggg ggatcgtcac ccacaacctg acccggatcg
     1321 cggccaaggt cgcggaagcg gggtcggccc ggtaagcgag gtaaggggcc aaccgggacc
     1381 gcggtgtgcc gccgcggggg tgggccgaag cgcgatttcg gcgccgagcg ccgaccccat
     1441 ttcgcaccgc aaactactga ccgctgcgca ataggggtca cccttcagtc cgcctagaat
     1501 cggggtatgg agccccgtga cgtactgccg gccgacggcc ttgagtggcg gagatttcgg
     1561 gcgctgcaat tgaggcgaga gggttggtcc cggcaggacg tcgccgcggc gctgggcgtc
     1621 gctcccgcga gcgtcagccg ttggttcgct cgtgcccgtg acgacggtcc ggaggccctc
     1681 cgcgcccgac cccgccccgg acgcccgccc aagctctcgg ctgctcagaa acgcctgatc
     1741 ccggagttcc tctggcatgg gacggaggcg tacgggtttc ggggtgaggt ctggacctgt
     1801 gcccgcgttg cgcgcgtgat cgaggaggag ttcggcgtcc actaccacaa ggaccacgtc
     1861 agccgactgc tccgggaact ccagtggacg ccccaggtgc cgatccgccg ggccatccag
     1921 cgggacgagg atgccatccg gcactggcgt gacacggtct ggccggatct gcaacggcgg
     1981 gcgcggcgcg agcgccgggt gctgatcttc gaggacgaat cggggttcta cctgctgccg
     2041 ggggtggtcc ggacgtacgc cccccagggg cagacaccgg tccttcgcga gacccggacg
     2101 cgcgaccacc tgtcggtcat gggcgggatg acgcccgagg gcaagctcta cgccctggcc
     2161 cggcaggagt cgctgaacgg cctggacacc atcgaattcc tcctgcactt ggggcgcgtg
     2221 gccggcgagc ggttgttggt gatctgggac ggctcgccga tccaccgccg gtcggaggtc
     2281 aacgagttcg tgtccgagac gcgcggcaag gtctggctgg aggccttacc gggttacgcc
     2341 ccggacctga acccatggga cgaggggggc tggcatcacc tgaaggacgt ggagatgcgc
     2401 aacttggtgt gtcgggactt ggaggagctg caccaggagt tccatctcgc ggtcggtcgc
     2461 ctccggcaga agccccggtt gatccgctcc ttctttgccc aggccggtct gaagatcgaa
     2521 taggggtaac cgctattgcg cagcggtcag taagaggagt ccagcccgct gccgccagtc
     2581 ccacatcttg gatggggact tgtacttcat gaacatcctc acgctggtct cgggcccgtt
     2641 gtactgctcc ctgtactcgg cgtcggcgaa tgctgcctct tcgcggagtc tgtcaagaat
     2701 gaccatagt
//
LOCUS       DBNGZC010000266         2346 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1000170, whole genome shotgun sequence.
ACCESSION   DBNGZC010000266 DBNGZC010000000
VERSION     DBNGZC010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1000170"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="AC16G0_04915"
     CDS             <1..663
                     /locus_tag="AC16G0_04915"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014663.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit C family protein"
                     /protein_id="HIR5098998.1"
                     /translation="TCLVARFGGYHGRPLQQGDVLPIGRPARPLERLIGWAAPEAWRQ
                     RHDSPATLRVVWGPQDDAVTEEGRRTFLESTYQVTPHTDRMGCRLEGSKIAHRNSADI
                     LSDWIPPGGIQVPGDGRPIILLVDRQTTGGYAKIATVIGPDIGRVAQCRPGDALRFRA
                     ISLDEAQEIAKSVEAGLAELPTHLIDTETWDQPAALGEAPEGVGMPFEKPAGGSAGTE
                     MP"
     gene            1008..1433
                     /locus_tag="AC16G0_04920"
     CDS             1008..1433
                     /locus_tag="AC16G0_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5098999.1"
                     /translation="MGTRLHVGVGLVGMVVLILMGCTIFGPPQTAPGPGVPHLANLRF
                     DPDVVQVGETTRMSFYFEVGSADIEEGFLIERGISQFQFYQSLNTIPIDLRKYAGVVA
                     DTVEIPLKWSNQGVRLFELYVVTQKGNASNRVRATVTVR"
     gene            1664..>2346
                     /locus_tag="AC16G0_04925"
     CDS             1664..>2346
                     /locus_tag="AC16G0_04925"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0033499 - galactose catabolic process via
                     UDP-galactose, Leloir pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HIR5099000.1"
                     /translation="MPELRKDPVTSRWVIISTERGKRPSDFGPELERKKTGFCPFCPG
                     NEDKTPSEVLAYRASGSPANGAGWHVRVVPNKFPALQIEGDLNRQGDGMYDKMNGIGA
                     HEVIIETPEHGETLASMSESRLEDILWAFRDRFLDLKRDMRFRYILIFKNYGEAAGAS
                     LEHPHSQLIATPIIPKRVQEELDGAKAYYNYKERCVFCDIVRQEAALGTRVILENAEA
                     LALAPYAPR"
BASE COUNT          419 a          719 c          812 g          396 t
ORIGIN      
        1 acctgcctgg ttgcccggtt cggcggctat cacggccggc cgctgcagca aggggatgtg
       61 ctgccgatcg gccggccggc gcgcccgctg gagcgtctga tcggctgggc ggcgccggag
      121 gcgtggcgac agcgtcatga ctcaccggcg acgcttcggg tggtttgggg tccccaggac
      181 gatgccgtca cggaggaggg acggcgaacc tttctggaat ccacctatca ggttacgccc
      241 cacaccgaca ggatgggctg ccggctggag ggatccaaga ttgcccaccg caactcggcg
      301 gacatcctct cggactggat cccgccggga gggatccagg ttccgggtga cggccggccg
      361 atcatccttc tggtggatcg gcagacgacc ggcggctatg ccaagatcgc cacggtcatc
      421 ggcccggaca tcggccgcgt ggcccaatgt cgtccaggcg acgcgctgcg cttccgggcg
      481 atctccctgg acgaggcgca agagatagcc aagagcgtcg aggcgggact cgcagagcta
      541 cccacgcacc tgattgacac cgagacctgg gatcagcccg ccgccctggg agaggcgccg
      601 gagggggtcg ggatgccctt tgaaaagcca gcgggcgggt ccgcggggac cgagatgcca
      661 taagccttct cgcgactgcg gtcgatggtg tcgtctcgta tttcgagtgg gcgcgggatc
      721 tccggcccct cctccggaaa gaagacgagg gaaaatcctg cctgcgtgat atactgacga
      781 ccggctttgg ggatgatggg cgcgggcatc caggaagacg gctggcctca ggacggcaac
      841 cccaccggag acggtgggcg ggtggcctcc gcgaccaagc tgcggggatc ctctcggtga
      901 caaccggagg cggcggatcg tccaggggaa ggcggtgcgt cggctctcca gaatcgggac
      961 cggtgcggag cagggggcgc cgttcggcct ttcacgaggg tgtcgggatg gggacgcgac
     1021 ttcacgtagg cgtggggctc gtagggatgg ttgtcttgat cctgatgggc tgtaccatct
     1081 tcggcccccc ccagacggcg cccggcccgg gcgtgccgca cctcgccaac ctgcgatttg
     1141 acccggacgt ggtccaggtc ggcgagacga cccggatgag cttctacttc gaggtgggat
     1201 cggcggacat cgaggaagga ttcttgatcg agcggggaat cagccagttt caattttatc
     1261 agagcctaaa cactataccc atcgacctcc ggaaatacgc cggcgtcgtg gccgacacgg
     1321 tggagatccc gctgaagtgg tcgaaccaag gggttcgcct cttcgagctg tacgtggtta
     1381 cccaaaaggg gaatgcgagt aatcgcgttc gggccaccgt gacggtgcgc tgagcctggc
     1441 gccccgcggg tgggccgccc ctagccttgc cgaggttccg gatcgcctac cgctcacgat
     1501 cggcctgatt cccccatact cctgacggcg agaaggacgg ttttgccgtc gcaagagagc
     1561 gtcatggggt atcggttccc acgcgtgcgc gccttcgcga tcttgacatt ggcgagtcgc
     1621 tcatttatgg ttcgtcctgc tgttgcgtct ggaaggagag tcgatgcctg agctccgaaa
     1681 agatccggta acctcacgat gggtcatcat ctccaccgag cgggggaagc gcccgtcgga
     1741 tttcgggccg gagctggagc gaaagaaaac cggcttttgc cccttctgcc ctggcaatga
     1801 ggacaagacg ccgagcgagg ttctggccta ccgcgccagc ggcagcccgg caaacggggc
     1861 ggggtggcat gtccgcgtcg tcccgaacaa gtttccggcg ctgcagatcg aaggggacct
     1921 gaatcgccag ggcgacggca tgtacgacaa gatgaacggc atcggcgccc acgaagtcat
     1981 catcgagacg ccggagcatg gcgagacgct ggcgagcatg tcggaaagca gactggagga
     2041 catcctctgg gccttccgcg accggttcct ggacctcaag agggacatgc gcttccgata
     2101 catcttgatc ttcaagaatt acggggaggc ggctggcgcc tccctggagc acccccactc
     2161 gcagctcatc gccaccccca tcattcccaa gcgcgtgcag gaggagctgg acggggccaa
     2221 ggcctactac aactacaagg agcgctgtgt cttctgcgac atcgtccggc aggaggcggc
     2281 cctgggaacg cgcgtcatcc tggagaacgc ggaagcgctg gcgctggcgc cgtatgcgcc
     2341 gcggtt
//
LOCUS       DBNGZC010000267         3617 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_412948, whole genome shotgun sequence.
ACCESSION   DBNGZC010000267 DBNGZC010000000
VERSION     DBNGZC010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_412948"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="AC16G0_04930"
     CDS             <1..233
                     /locus_tag="AC16G0_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HIR5099001.1"
                     /translation="CTIPPFVTWALGVHVFRINPAVLMGGVAGARSHSGPCREAAKEI
                     NSSVPWVGFPVGYAVSGVLLTVFGYFAMVLSQ"
     gene            365..2056
                     /gene="aspT"
                     /locus_tag="AC16G0_04935"
     CDS             365..2056
                     /gene="aspT"
                     /locus_tag="AC16G0_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010992484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0070906 - aspartate:alanine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-alanine antiporter"
                     /protein_id="HIR5099002.1"
                     /translation="MDYVVRALRENPELAVFLTLAVGFLIGRLKIGSFSLGTVVGTLL
                     AGVVIGQLNIKVPSTVKSIFFLLFLFSTGYKVGPQFFRGLKRDAVPQVALTVVLCTSC
                     LLTAFMFAKLFGYDIGTAAGLLAGAFSESTVIGTAGDAIQRLAISDAEKTALLNNIPV
                     AYAVTYLIGAAAVVWFLPTIGPKLMGINLREEGARQQAEVAGSAELGLGVTSAARHFD
                     IRAYRVTNPVLVDKTVAELEALPTDIRVFILRLRHKGEIVEVTPTSVIRQDDLVAVIA
                     RQEAHVERGAKIGPEVDDKALLDIPVESLDVVETHRAYVGKTLAELTGLAAARGVFLR
                     KLTRAGQEIPMSAGLRIDRGDVLHLIGTKPEVERAAKELGYPDRPTTATDMIFVGTGI
                     VIGGLVGLLSVTVLGVPLTLTASGGALIMGLVFGWLRSVYPYFGRIPEPAIWIFDTVG
                     LCVFIGVVGLSAGPTFISGLKTTGISLVLVGLVSALLPHVVGVLFGRYVLKMNPLVVL
                     GACAGAGTMTAALRAVQDEARSSVPALGYTVPYAIGNILLTAWGPVIVAMMSVGK"
     gene            2340..2714
                     /locus_tag="AC16G0_04940"
     CDS             2340..2714
                     /locus_tag="AC16G0_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099003.1"
                     /translation="MQARIMIGVGALMLAVVVLPSGVWGATIDDVKAEMKGMTPPSGA
                     KLELRVPREIYGKTRMPIDQLCVSDGRLRPIKGSGSDAVKDMGPVKPGKTYQVTIVEK
                     FASATGDEKIRATRKVSIPDCK"
     gene            3157..>3617
                     /locus_tag="AC16G0_04945"
     CDS             3157..>3617
                     /locus_tag="AC16G0_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099004.1"
                     /translation="MRYRIGRFWSSDVGLSALLVALILIVFILRPLVALGLAGGRVIE
                     LTFAFVMLSGAWSVWGGRTRMTIIVLGAGTAVALRWLYWAGASPRLAPWAALASAMIA
                     GMLMALVLRRVFQPGPVTWHRIQGAIAVYLVFGLAWAQVYEGVEALAPGAF"
BASE COUNT          602 a         1074 c         1249 g          692 t
ORIGIN      
        1 catgcacgat cccgcccttt gtcacctggg cgctaggggt ccacgtgttc aggatcaacc
       61 cggcggtgct gatgggcggc gtggcgggcg cccgctcgca ctccggaccc tgccgcgagg
      121 cagccaagga gatcaacagc tcggtgccgt gggtcggttt cccggtcggc tatgcggtgt
      181 caggggttct cctgacggtg ttcggctact tcgccatggt cctgtcgcag tagactgccg
      241 actctcgacg gggcgcccga aggactactg tgggcttccg gcgccctgtt cgagcggctt
      301 ggcggtcggg gcaccggaag cacgaatcct ctggtcggac gcatatcatc ggagggaaca
      361 gacaatggac tatgtggtgc gggctttgcg ggagaatccg gagctcgcag ttttcctcac
      421 ccttgctgtc ggattcctta ttggccggct caagatcggg agcttcagcc tgggcacggt
      481 cgtcgggacg ctcctggcag gtgtggtgat cgggcagttg aacatcaagg ttccctccac
      541 cgtgaagtcg atcttcttct tgcttttcct cttcagcacc ggatacaagg tcgggccgca
      601 gttcttccgc gggctgaagc gcgacgccgt gccacaggtg gcgctcaccg tcgtcttgtg
      661 cacctcgtgc ctgctcacgg cgttcatgtt cgcgaagctt ttcgggtacg acatcggaac
      721 ggcggcaggc ctgctggcgg gggcgttctc ggagtccacg gtgatcggca ccgcaggtga
      781 cgcgatccag cgcctggcga tctcggatgc ggagaagacc gccctcttga acaatattcc
      841 ggtcgcctat gccgtgacct acctcatcgg ggctgccgcc gtggtctggt ttctccccac
      901 gatcgggcca aagctgatgg ggatcaactt gcgggaggag ggtgcccggc agcaggccga
      961 ggttgcaggc tcggcggagc tggggctcgg ggtcacgtcg gccgccagac acttcgatat
     1021 ccgtgcctat cgcgttacca acccagttct cgtcgacaaa accgtagcgg aactcgaggc
     1081 gctgcccaca gatatccgcg tattcattct ccgtttacgc cacaagggcg agatcgtcga
     1141 ggttacgccc acctcggtca tccgccagga cgatttggtc gctgtcatcg cccggcaaga
     1201 agcgcacgtc gagcgcgggg ccaagatcgg tcccgaggtg gacgacaagg ccctcctcga
     1261 catcccagtc gagtcgctgg atgtcgtgga aacgcaccgc gcctatgtgg gaaagactct
     1321 cgcggagctg acggggctcg ccgcagcccg cggggtgttc ctgcggaaac tcacgcgggc
     1381 cgggcaggag attcccatgt ccgccggact gcgcatcgat cgaggggacg tgctgcactt
     1441 gatcggaacc aagcctgagg tcgagcgggc ggcgaaagaa ctggggtatc cggaccgtcc
     1501 caccacagcg acggacatga tcttcgtcgg caccggcatc gtgatcggag gcctcgtcgg
     1561 cctgctctcg gtcaccgtct tgggcgtgcc cctgaccctc accgccagcg gcggtgccct
     1621 gatcatgggg ctggtctttg gatggctgcg gtcggtgtat ccgtacttcg gccggattcc
     1681 cgagccggcg atctggatct tcgataccgt cggattgtgc gtgttcatcg gcgtggtcgg
     1741 cctgagcgcg ggcccgacct tcatctccgg gctgaagacg acgggcatca gcctggttct
     1801 cgtgggcctg gtgtcggccc tgctgccgca cgtcgtgggg gtcctcttcg gtcgctacgt
     1861 tctgaagatg aacccgctcg tggtcctcgg cgcctgcgcc ggggccggga cgatgacggc
     1921 ggcccttcgc gccgtccagg acgaggcccg gagcagcgtt ccggcgctgg gctacaccgt
     1981 gccgtatgcc atcgggaaca tcctgctgac ggcctgggga ccggtcatcg tggcgatgat
     2041 gtcggttgga aaatgagccc agtccgaatt attcacaaac ttcatcatga acaatccggc
     2101 caatgagcaa gtcccaatga ccaacgcaag cagcacttga atgagatcat ggctacaacg
     2161 ccaccaaggg actggagttg cagtagcccg ccgcgctggc ggcgctgaag aagtagccga
     2221 gtggtaatat ttgtgaacgc cggttgaagg cgagcttggc cgtggtggtc agggctcacc
     2281 ccggccggag atgcgggcgt gcggacctcg cggggggtcg gccgccagag gagattgcca
     2341 tgcaagcgag aatcatgatt ggtgtcggtg cgctcatgct ggccgtcgtc gtgttgccca
     2401 gtggtgtgtg gggcgcgacc atagatgacg tgaaagccga aatgaaggga atgaccccac
     2461 cctcgggggc caaactcgag ttgcgcgtcc cgcgtgagat atacggaaaa acgcgaatgc
     2521 ccatcgacca actctgtgtg tccgacggcc ggctccgccc gatcaaaggc tcgggctcag
     2581 atgcggtcaa ggatatgggt ccggtcaagc ccggcaaaac gtaccaagtg acgatcgtcg
     2641 aaaaattcgc gagtgcaaca ggggacgaga agatacgggc tacgcggaaa gtgtccatcc
     2701 ccgactgcaa gtaatgcgaa gtgcgggtgc ggggtgggtg cgtgatcccg aagacacaat
     2761 accggcctgt ggctggtcca cagtcgccat gagaggagat acggggcacg cccggctttg
     2821 tcgtgaaggg tccaatgatc cggttcggcg tgcaaacggt gtcggtgaag acacgcgtcg
     2881 cgacggggac aggtgggggg ccccagctac ggacgatgcg ttctccgaat cccccgcgac
     2941 gcgcaccagc tcggctgcac gatcgaggtc ggcaagatgt tgggcgacgg caagaccagc
     3001 tggttccgcg cgtccttcgt caagccattc gaggtattgc cctgcgcatg agtgtcgcag
     3061 gggtcgcggc gggatattcg gatccggctt atcgcgaact acgagttcaa tacacaggaa
     3121 gcggcagatg cggctggcgt ggggtggagg agagcgttgc gctataggat agggcggttc
     3181 tggtcaagcg atgtcgggct cagcgcattg cttgtggcgc tgatcctgat cgtcttcatc
     3241 ctgcgtccgc tggtggctct cgggctggcg ggcgggcgcg tgatcgagct gacgttcgcc
     3301 ttcgtgatgc tctcgggcgc atggagtgtg tggggcgggc ggacgcggat gacgatcatc
     3361 gtgctcggcg cggggactgc cgtggcgctg cgctggcttt actgggctgg cgcctcgccg
     3421 cgtctggccc cctgggcggc gctggcctcc gccatgatcg ccgggatgct catggcgctg
     3481 gtcctgaggc gggtgttcca gcccgggccg gtgacctggc atcggatcca gggcgcgata
     3541 gccgtgtacc tggtgtttgg actcgcctgg gcccaggtct acgaaggcgt ggaagcgctc
     3601 gcacccggcg ccttcca
//
LOCUS       DBNGZC010000268         1846 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_705591, whole genome shotgun sequence.
ACCESSION   DBNGZC010000268 DBNGZC010000000
VERSION     DBNGZC010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_705591"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="AC16G0_04950"
     CDS             <1..218
                     /locus_tag="AC16G0_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099005.1"
                     /translation="RGLVAPGGLPEDARKVLEDAMLKYTKTQIYKDYIKENMLSEAWM
                     DGPTFGKWNEKEYGRYQVMLKDMGLLK"
     gene            324..782
                     /locus_tag="AC16G0_04955"
     CDS             324..782
                     /locus_tag="AC16G0_04955"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR5099006.1"
                     /translation="MRRADQITGIIMLIFSAALIEGSRRMPPSNTFGPGAGFLPFWLG
                     VFMAVFSILLIVNATRQPVKSGGGRPFPTGRPVLSILGTVGGLSAFILLLESLGFLLS
                     TALLTAFILRVVESERWPTTVVVSVANAIGLYIVFQVLLGAGLPKNFLGF"
     gene            831..>1846
                     /locus_tag="AC16G0_04960"
     CDS             831..>1846
                     /locus_tag="AC16G0_04960"
                     /inference="COORDINATES: protein motif:HMM:NF014073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR5099007.1"
                     /translation="MGLFDNILLGFQVALSWQNLLFCLIGVTYGTLIGVLPGIGPVVG
                     VSILIPVTFGLNATTAIITMAGVYYGAMYGGSTTSILINVPGESASVVTCLDGYQMAR
                     KGRAGPALGMAALASFFAGTLSVVLLTLIAPPLADVALSFGPPEYFALTLMGLTLVTS
                     LAGESMVKGLMSAVFGLIVACVGVDAVSGEERFTFGMLSLLDGMGFVGVAVGLFAVSE
                     VLCNLEESMEQVFVKAKLGLRALFPNLQDWKDSWWPMWRSSFLGFFVGNLPGAGATIA
                     SFMAYGMEKRLSKHPERLGTGAIEGVAAPEAANNAAAGGAMVPLLALGIPSSGTGAVL
                     LGAL"
BASE COUNT          291 a          556 c          610 g          389 t
ORIGIN      
        1 atcgtggact tgtggccccg ggcggtctcc cggaagatgc gcgcaaggtg ctggaagatg
       61 ccatgctgaa atatacgaag acccaaatct acaaggatta catcaaagaa aatatgctct
      121 ccgaggcctg gatggacggt ccgacttttg gcaaatggaa tgagaaagag tacggccgtt
      181 accaggtgat gctgaaggac atgggcctgt tgaagtagac gcgaggattt cccgggcgca
      241 gcgtccgact cggcgctgcg cccggcctca cctgaacccc gccggacatt cgacgtggct
      301 gtgggcagag acggaggcgg agcgtgcgac gagccgacca gataaccggc atcatcatgt
      361 tgatcttctc cgcggccctg atagagggaa gccgccggat gccgccgagc aataccttcg
      421 gtccgggggc cggattcctg cccttctggc tcggggtatt catggcggtc ttctccatct
      481 tgctgatcgt caacgcgacg cgccagccgg tgaagagcgg gggcggccgt cctttcccga
      541 cgggccggcc tgtcctgtcc atcttgggaa ccgtgggagg cctgagcgct ttcatccttc
      601 tcctggaatc cctcggcttt ctgctgtcca ccgcactgct gaccgccttc atcttgcgcg
      661 tcgtggagag tgagcggtgg cccacgaccg tcgtggtttc ggtggccaac gccatcggac
      721 tgtatattgt cttccaggta ctcttggggg cgggccttcc gaaaaatttc ctgggtttct
      781 gagcggagcg caggcctttg gtcgcggagg gatgaaaagg gagaggtcgc atggggcttt
      841 tcgataacat cctgctgggc tttcaggtgg cgctctcgtg gcagaatctc ctcttctgcc
      901 tcatcggcgt cacctatggc accttgatcg gcgtcttgcc cgggatcggg cccgtggtcg
      961 gggtctccat cctcattcct gttaccttcg gcttgaacgc cacgacggcc atcatcacga
     1021 tggccggggt gtactacggt gccatgtacg gcggctcgac tacgtccatc ctcatcaacg
     1081 tgcccggcga atcagcatcg gtcgtcacct gcctcgacgg gtaccagatg gctcgcaagg
     1141 gacgggccgg tcccgcgctg ggcatggccg cgttggcctc cttcttcgcc ggcaccctct
     1201 ccgtggtcct gctcacgctc atagccccgc cgctggccga cgtcgccctc tcctttgggc
     1261 cgccggagta ctttgccctg accctcatgg ggctgaccct ggtgaccagc cttgccggcg
     1321 agtccatggt gaagggactc atgagcgccg ttttcggcct gatcgtcgcc tgcgtggggg
     1381 tggacgccgt gagcggggag gagcggttca ccttcggcat gctttccctc ctcgacggca
     1441 tgggattcgt cggcgtggcc gtggggctct ttgcggtgtc cgaggtcctg tgcaatctcg
     1501 aggaatcgat ggaacaggtg ttcgtcaagg caaagctggg tctccgggct ctcttcccca
     1561 atctgcagga ctggaaggac tcctggtggc caatgtggcg cagctcgttc ctggggttct
     1621 tcgtggggaa tctgccgggg gcgggggcca ccatagcctc attcatggcg tatggcatgg
     1681 agaagaggct gagcaaacac ccggagcgat tggggacggg agccattgaa ggcgtggccg
     1741 cgcctgaggc agccaacaac gcggccgccg ggggggcgat ggtgcccctc ctggccctgg
     1801 gcattccgtc gtcagggacg ggcgcggtac tcctgggggc tctctt
//
LOCUS       DBNGZC010000269         4771 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2076102, whole genome shotgun sequence.
ACCESSION   DBNGZC010000269 DBNGZC010000000
VERSION     DBNGZC010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2076102"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1133)
                     /locus_tag="AC16G0_04965"
     CDS             complement(108..1133)
                     /locus_tag="AC16G0_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099008.1"
                     /translation="MERWWWPLAGLMVLAVATVAAAQENVLVLYGSPDLDWVAAVAAK
                     FEKDTGIKVQWIRASSNEMYARIEAEKVNPKGDVWFGGTGDPHFDAAEKGLTEPYCSP
                     KMAELREFMRDPIGGCRTLGLYAGPLGWAVNEGLLKKLGKPMPQTWDDLAKPDYKGLI
                     AIANPNTSGTAYTTLATILQLKGEEKGWEYLAKLHKNVAQYAKSGSAPGQLAGRGEVA
                     IAVIFLHDVVMFAEEGFPLKAVSPADGTGYEIGGLSLIKGAPHMANAKKFIDWALTPE
                     TQLIAKDFRSFQLQSNKNTPLPAVVIKNGVDFEHVKTIKYDFMWASKNRDRILKRWTE
                     QIFSQAR"
     gene            1355..2335
                     /locus_tag="AC16G0_04970"
     CDS             1355..2335
                     /locus_tag="AC16G0_04970"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5099009.1"
                     /translation="MRTSEAEMPARILVVDDEPMNLELMQEMLTSAGYEVSAAGGGEE
                     GLAAALQSKPDLILLDLMMPVLSGFEVCARLKSRPETQDIPIIFVTALTTAGTKERAL
                     AAGGDDFIGKPVQLPELLTRVEALLKVRHLNRDLDQALASLHERAPAQPGKTVREPVA
                     PPAGSAAAILVVDDEALTRRLYSDVLQEAGYTCYTAEGGAQALEIARQQAIDVVLLDI
                     MMPARSGLEVLADLGERIPDSPVIIVTAHPSSDNAIAALRLGAFDFIVKGFKSEVMLA
                     TVARALARRRLAVRNRQMIRQLEAKIQDLLALVRGYAEEPDASARLHPKG"
     gene            complement(2398..3159)
                     /locus_tag="AC16G0_04975"
     CDS             complement(2398..3159)
                     /locus_tag="AC16G0_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009278563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR5099010.1"
                     /translation="MAEVENTLAVFIDFENLALGFKHSKEEFDIQRVLERLVEKGKLI
                     VKVAYADWSRYAKYKQDLHEAAIELIEIPKRTATGKNSADIRLCVDAMDLCYSKAHIG
                     TFVIVSGDSDFSPLVSKLKENGKSVIGLGMKGSTSTLLSDNCDEFIFYENLGTPDRIT
                     PPGVREIPKEKREVFELLFDTIAGLIRENKEILWSSMIKDTMKRKRPAFSERAYGYRT
                     FSDLLEDAQKQGFIELRTDPTSGTYVVVGFAKPGR"
     gene            complement(3320..4681)
                     /locus_tag="AC16G0_04980"
     CDS             complement(3320..4681)
                     /locus_tag="AC16G0_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HIR5099011.1"
                     /translation="MPETKVVLSEKDLPTRWYNLQADLPFPLPPVIHPGTKQPIGPQD
                     LAPLFPLELIKQEVSRERWVAIPEPVRDIYRLWRPTPLYRAHRLEKALDTPARIYYKW
                     EGVSPAGSHKPNTAVAQAYYNQQEGVRRITTETGAGQWGSALALATRQLGLECKVYMV
                     KISYEHKPYRRIMMESWGASVVPSPSSDTQAGRAILAKDPASPGSLGIAISEAVEDAA
                     TRPDTKYSLGSVLNHVLMHQTVIGLEAKRQFEVLGEYPDILVGCVGGGSSFAGFTFPF
                     VQDKLSGAAPGLRIVAVEPTACPSLTKGKYLYDFGDTAGTTPLMKMYTLGHSFIPAPI
                     HAGGLRYHGMAPLVCVLYDQKIIEAVAVRQNPIFEAALTFARTEGIIPAPEPSHAIRV
                     VIDEAVRCRERGERKTIAFVLCGHGHFDLASYERYLAGQLPDYELPQSAIDEALSLVP
                     RVE"
BASE COUNT          830 a         1570 c         1456 g          915 t
ORIGIN      
        1 agcggtcaca ggggcacccc agaagctgtc atggttcagc ccccgccctc ccctcccccc
       61 gcaagggggg aggggaggga aatgggcggc tgatcacagc tgtcttgtca gcgcgcttgc
      121 gagaagattt gttccgtcca gcgcttcagg atccgatccc gattcttgga cgcccacatg
      181 aagtcatact tgatggtctt cacgtgctcg aaatccaccc cgttcttgat gaccacggcc
      241 ggcagcggcg tattcttgtt ggactggagc tggaaggatc ggaaatcctt ggcgatcagt
      301 tgagtctccg gcgtcagcgc ccagtcgatg aacttcttgg cgttggccat gtggggtgcg
      361 cccttgatca ggctgaggcc gccgatctcg tagccggtgc cgtccgccgg ggacacggcc
      421 ttcaatggaa atccctcctc ggcgaacatc acgacgtcgt gcaggaagat gacggcgatg
      481 gccacctcgc cccgcccggc cagttgtccc ggcgccgagc cggacttggc gtactgggcg
      541 acattcttgt gtagcttggc cagatactcc cagcccttct cctcaccctt cagttgcagg
      601 atggtcgcca gcgtcgtgta ggcggtgcca gaggtgttgg ggttggcgat ggcgatcaga
      661 cccttgtagt cgggcttggc gaggtcgtcc caggtctggg gcatgggctt ccccagcttc
      721 ttcaagagcc cctcgttcac agcccacccc agcggcccgg cgtacaggcc gagcgtacgg
      781 cagccaccga tgggatcgcg catgaactcg cgaagctccg ccatcttggg cgagcagtag
      841 ggctccgtca gccccttctc cgccgcatca aaatgcgggt cgcctgtccc cccgaaccac
      901 acgtctccct tggggttcac cttctccgcc tcgatccggg cgtacatctc gttgctgctg
      961 gcccggatcc actgcacctt gatacccgtg tctttctcga acttggccgc cacggcggct
     1021 acccaatcca gatccgggct gccgtacagg accaggacgt tctcctgcgc cgccgcgacc
     1081 gttgccacgg cgaggaccat caatccggcc agaggccacc accagcgttc cataagaccc
     1141 tccttttcct cgaaggtaag ctgaatggtc tgaacggccg ttacgttagc aagaacgcaa
     1201 ccggggattc aaggccaaac gttgcgtccc ggaaagtcat ccgcggaaat agacagccag
     1261 gacaggctcg attgacacgc cgatatcttt catctaggtt tctacctcca tgaccgacgc
     1321 catggatcat accgcgaacg gagaggaggc gggaatgaga acttcggagg cggagatgcc
     1381 agcccggatc ctcgtggtgg atgatgagcc gatgaacctg gaactgatgc aggaaatgtt
     1441 gacatccgcc ggttacgagg tctcggcggc cggcggcggg gaggagggcc tggccgcggc
     1501 cctgcagagt aaacccgacc tcatcctcct cgacctcatg atgccggtcc tctcgggatt
     1561 cgaagtctgc gcccgactca agtcacgccc ggagactcag gacatcccga tcatcttcgt
     1621 gacggcgctc accaccgccg ggaccaagga acgggccctg gcggcaggcg gcgacgattt
     1681 cattgggaaa ccggtccagc taccggaact tctcacccgg gtcgaggccc tgctgaaggt
     1741 gcggcatctg aaccgcgacc tggaccaggc cttggcctcc ctgcacgagc gggcaccggc
     1801 tcagcctggc aagacagttc gggagcccgt cgcccctccg gccggttccg ccgccgccat
     1861 cttggtggtg gatgacgagg ccctcacgcg gcgactgtac tcggatgtgc tgcaggaggc
     1921 gggctacacc tgctacaccg cggagggcgg ggcccaggcc ctggagattg cacgccagca
     1981 ggccattgac gtcgtgctgt tggatatcat gatgcctgct cggtccggcc tggaggtcct
     2041 ggcggacctg ggcgagcgaa tccctgactc cccggtgatc atcgtgacgg cgcatccgag
     2101 ctctgacaac gccatcgccg cgctgcgcct gggcgccttt gatttcatcg tgaaggggtt
     2161 caagagcgag gtgatgctgg cgacggtcgc acgcgccctg gcccgtcggc gcctggccgt
     2221 ccgaaatcga cagatgatcc gtcaactcga agccaagatc caggatctgc tggccctcgt
     2281 ccgagggtat gccgaagagc ccgacgcttc ggcccgcctc caccccaagg ggtaaggccc
     2341 gcctccaccc aattcgaaat tcgaatttcg aaactcgaga tctatttcct tccagcgtca
     2401 gcgccctggc ttggcgaaac ccaccaccac gtaggtcccg ctggtcggat cggtccgcag
     2461 ctcgatgaag ccttgcttct gggcatcctc caggagatcg ctgaatgtcc ggtagccgta
     2521 ggcgcgttcg gagaaggccg gtcgcttgcg cttcatggtg tccttgatca tcgaggacca
     2581 gaggatctcc ttgttctccc ggatcagccc ggcgatggtg tcaaacaaca gctcgaagac
     2641 ctcccgcttt tccttgggga tctcccggac gcccggcggc gtgatccgat cgggggttcc
     2701 caagttctcg tagaagatga actcatcgca gttgtcgctc aggagcgtgg acgtcgagcc
     2761 cttcattccc aagccgatga cgctcttccc gttctccttc aatttggaga ccaggggaga
     2821 gaagtcgctg tcgccggaga cgatcacgaa ggttccaatg tgggccttgg agtagcacag
     2881 atccatggca tccacgcaga gccggatgtc cgcggagttc ttaccggtcg cggtccgctt
     2941 gggaatctcg atcagctcga tggccgcctc gtgcaggtcc tgtttgtact tcgcgtaccg
     3001 gctccagtcg gcgtacgcga ccttgacgat cagcttgccc ttctccacca gtcgctccag
     3061 gacgcgctgg atgtcgaact cctccttgga atgcttgaat cccagcgcca ggttctcgaa
     3121 atcgatgaaa accgccaggg tgttctcaac ctcggccatc actctctcct catcccctcg
     3181 cctcgaattc tcgcccaggg gcaccctcgg ccggcaggcg ggtcagggcc aatgaaaaac
     3241 tcacaccaac aacggacagc cggccaatcg gggttgacca ttgtcgactg tctgttgtgc
     3301 attcttgcgt gggtcctcct cattcgactc tgggcaccag gctcaacgcc tcatctatgg
     3361 ccgactgggg cagctcgtaa tccggtagct gtcccgccag gtatcgctcg tacgacgcca
     3421 ggtcgaagtg cccgtgcccg cataggacga aagcgatggt cttccgctcc ccccgctcgc
     3481 ggcagcggac ggcctcgtcg atcaccaccc gaatggcatg agacggctcc ggcgccggga
     3541 tgatgccttc cgtccgggcg aacgtcaggg ccgcctcgaa gatcggattc tggcggaccg
     3601 ccactgcctc gatgatcttc tgatcgtaca ggacacagac cagcggggcc atgccatggt
     3661 agcgtaaccc tccggcgtgg atcggcgccg ggatgaagga atgcccgagg gtgtacatct
     3721 tcatcagagg ggtcgtcccc gccgtgtccc cgaagtcgta gaggtacttg cctttcgtca
     3781 ggctgggaca ggcggtaggc tccaccgcca cgatgcgcag ccccggagcc gctccggaca
     3841 gcttgtcctg aacgaagggg aaggtgaacc cggcgaagga gctcccgccg ccgacgcagc
     3901 ccaccaggat gtccggatac tcccccagga cctcgaattg tcgcttggct tccaagccga
     3961 tgaccgtctg gtgcatcagc acgtggttca gcacgcttcc cagggaatac ttcgtgtccg
     4021 gacgggtcgc ggcgtcctcc accgcctcgc tgatggcgat gccgagactc cccggcgagg
     4081 cggggtcctt ggccaggatg gcgcgcccgg cctgggtgtc cgagctgggg ctgggcacca
     4141 cggacgcccc ccagctctcc atcatgatcc gccggtaggg cttgtgttcg tagctgatct
     4201 tgaccatata gaccttgcac tctagcccca actgtcgggt ggccaaggcc agcgcactgc
     4261 cccactgccc cgcgcccgtc tccgtggtga tccgccgcac accctcctgc tggttgtagt
     4321 aggcctgcgc cacggccgtg ttgggcttgt ggctgcccgc ggggctcacc ccctcccact
     4381 tgtagtagat gcgggcgggt gtatccagcg ccttctccag tctgtgagct cggtacaacg
     4441 gcgtgggtcg ccacaggcga tagatgtctc gcaccggctc ggggatcgcc acccatcgct
     4501 cgcgactcac ctcttgcttg atcagctcca gcgggaaaag gggagcgaga tcctgggggc
     4561 cgatgggctg cttggtcccc ggatggatca ccgggggaag gggaaagggg aggtccgcct
     4621 ggaggttgta ccacctggtt ggcaggtctt tctctgagag aaccaccttg gtctcgggca
     4681 tcagccgcct cccttcagcc tgtaattatc acgcctcagc cgtcggccgc gaacacagag
     4741 ccctgcccga cagcgatttc cggcagcgac g
//
LOCUS       DBNGZC010000270         1258 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2076143, whole genome shotgun sequence.
ACCESSION   DBNGZC010000270 DBNGZC010000000
VERSION     DBNGZC010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2076143"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="AC16G0_04985"
     CDS             complement(<1..341)
                     /locus_tag="AC16G0_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099012.1"
                     /translation="MTLHWLTGTDLAIALAAFLLLWYVVGGFMNRRRAGMIVRQVRDS
                     IESLGGTATIRWIGRNAFRIEVEKLTAPFEKLSISGLLEPRETFFLWLVWRLSGRQDW
                     LMIRATLNGAVG"
     gene            complement(451..729)
                     /locus_tag="AC16G0_04990"
     CDS             complement(451..729)
                     /locus_tag="AC16G0_04990"
                     /inference="COORDINATES: protein motif:HMM:NF052803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7990 family protein"
                     /protein_id="HIR5099013.1"
                     /translation="MTFREWWEGWRLWFRGFLYGMALYDGVMMFRKQRADMEHLFVLI
                     VFGDLVGVPVIPPYYTLRLLPYIVPAITNWKRRLLRERDLTDLAAGAG"
     gene            complement(862..>1258)
                     /locus_tag="AC16G0_04995"
     CDS             complement(862..>1258)
                     /locus_tag="AC16G0_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5099014.1"
                     /translation="HLVVGLPIVIWVMIGFFEDIPAELREAALIDGCTDYGAFLRVAV
                     PLVKPGIVATAILSFIFSWNNFLFSVILAGRNTRTLPIAVFNMIGYEEINWGPLAAAA
                     TMITLPVIILTLIIQRHIVTGLTFGAVKG"
BASE COUNT          253 a          401 c          392 g          212 t
ORIGIN      
        1 cccacggcgc cgttcagggt cgcccggatc atcagccagt cctggcggcc actgagccgc
       61 cacaccagcc agaggaagaa ggtctcccgg ggctccagca atccgctgat gctcagcttc
      121 tcgaacggag cggtgagttt ttccacctca atgcgaaagg cgttccgccc gatccagcgg
      181 atggtggcgg tgcccccgag ggattcaatc gagtctcgga cctgccgcac gatcatgccg
      241 gcacgccggc gattcatgaa acccccgaca acgtaccaaa ggagcaggaa cgcagccaac
      301 gcgatcgcga ggtcggtgcc ggtcagccag tgtagtgtca tcagcgggcc atcccgaaaa
      361 cacgggggct ggaagctggg ggctgggctc gaggtattgg tgggagtgtg gattggcgga
      421 tccgcctctt ggcggcccct gattgctgca tcatccggca cccgccgcga ggtcggtgag
      481 gtccctctcg cgcagcaatc gtcgcttcca gttggtgatg gccgggacga tgtacggaag
      541 gagccgcagg gtgtaatacg gcggaatcac cggaacgccg accaaatcgc cgaacacgat
      601 gaggacgaag aggtgctcca tgtccgcccg ctgcttccgg aacatcatga ccccgtcgta
      661 cagggccatg ccatacagga aaccccggaa ccagagccgc cagccttccc accattctcg
      721 gaatgtcatc ggatgcgctt ccaatccccc ttggtctgtg atcctcgatc cccagctccg
      781 gagccatgct gtcggcccgc cttgcgtaga gtatggctcg ccggcggcac cccgtcaacc
      841 ggtcgccatc ccagcgtcac gctacccttt caccgctccg aaggtgagac cggtgacgat
      901 gtggcgttga atgatcaggg tgaggatgat gaccggcagg gtgatcatgg tggcggcggc
      961 ggccagaggt ccccagttga tctcctcgta cccgatcatg ttgaagactg cgatgggaag
     1021 cgtccgggtg ttccgccccg ccaggatcac cgagaacagg aagttgttcc aggagaagat
     1081 gaagctcaag atggccgtgg ccacgatccc cggcttcacc aaaggaacgg ccactctcag
     1141 gaaggcccca tagtcggtgc aaccgtcgat aagcgccgcc tctcgtaact cggccgggat
     1201 gtcctcgaag aacccgatca tgacccagat gacgatgggc aggcccacca ccaggtgg
//
LOCUS       DBNGZC010000271         1261 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2369425, whole genome shotgun sequence.
ACCESSION   DBNGZC010000271 DBNGZC010000000
VERSION     DBNGZC010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2369425"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..661)
                     /locus_tag="AC16G0_05000"
     CDS             complement(185..661)
                     /locus_tag="AC16G0_05000"
                     /inference="COORDINATES: protein motif:HMM:NF014808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5099015.1"
                     /translation="MEIRETSDLLQEVPAEVLVLFHLADEPSPRGRLGRVDWILGSAI
                     SRLRARGKFAGERGTSALLSPNGKIKAERILVLGLGNQADLSMPALYRLSYQAAHTIL
                     NLRCVVILLDLPFRAFPLSPPDRIRRAFLEGFQAELKRGRPDAPYRITLLPAEIPA"
     gene            complement(663..1088)
                     /gene="nusB"
                     /locus_tag="AC16G0_05005"
     CDS             complement(663..1088)
                     /gene="nusB"
                     /locus_tag="AC16G0_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR5099016.1"
                     /translation="MGKRRKARELALMILYELEFRPGEVKAILESFWQGHPVPEDVRA
                     FTESLVRGTVETGPELDRQIAAHAAHWSLARIALVERNILRLAAYELLYRDDIPERVA
                     INEAIELAKLYGSQESGAFVNGILDQIRLSLRPPQRAPA"
     gene            complement(1167..>1261)
                     /gene="ribH"
                     /locus_tag="AC16G0_05010"
     CDS             complement(1167..>1261)
                     /gene="ribH"
                     /locus_tag="AC16G0_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743986.1"
                     /note="RibE; 6,7-diimethyl-8-ribityllumazine synthase;
                     DMRL synthase; lumazine synthase; beta subunit of
                     riboflavin synthase; condenses
                     5-amino-6-(1'-D)-ribityl-amino-2,4(1H,3H)-pyrimidinedione
                     with L-3,4-dihydrohy-2-butanone-4-phosphate to generate
                     6,6-dimethyl-8-lumazine (DMRL); riboflavin synthase then
                     uses 2 molecules of DMRL to produce riboflavin (vitamin
                     B12); involved in the last steps of riboflavin
                     biosynthesis; forms a 60mer (icosahedral shell) in both
                     Bacillus subtilis and Escherichia coli; in Bacillus
                     subtilis this 60mer is associated with the riboflavin
                     synthase subunit (alpha) while in Escherichia coli it is
                     not; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR5099017.1"
                     /translation="VERAGTKGGNKGFDAACSAIEMVNLFRELK"
BASE COUNT          210 a          411 c          407 g          233 t
ORIGIN      
        1 caagtgggtg ggtccatccg atccagtgga tggatttctc cctctgactg cctcccctgg
       61 ttcatctgca acgcttccat cctcgccctc gcgtcccttt gcaggaaccc gccgctcctc
      121 gggcacgccc ccgattcatg tcacctcatc cgcctttgtg cgttttgggg tgagccgctt
      181 gggctcaagc aggaatttcc gcggggagga gggtgatccg gtagggcgcg tcgggccgcc
      241 cgcgtttgag ttccgcctgg aagccttcga ggaaggcacg ccggatccga tccggcggcg
      301 aaagcgggaa cgcccggaag ggaaggtcga ggaggatgac aacacaccgc aggttcagga
      361 tggtgtgggc ggcctgatag gacagccggt agagggccgg catggacaga tcggcctgat
      421 ttcccaggcc gagtaccagg atccgttccg ccttgatctt gccattggga gacaggaggg
      481 cgctcgtccc gcgctcgccg gcgaactttc ctcgggcgcg aagccgggag attgcgctgc
      541 ccaggatcca atccacccgg cccagccggc cgcgcggcga gggttcatcc gcgaggtgaa
      601 agagcaccag gacctctgcg gggacttctt gcagcaggtc ggaggtctcg cggatctcca
      661 tctcaggcgg gggctcgctg aggcggccgg aggctcaggc ggatttggtc caagatcccg
      721 ttcacgaaag caccggactc ctggctgccg tagagcttgg cgagctcgat ggcctcgttg
      781 atggccaccc gttccgggat gtcgtcgcgg tacagcagct cgtaggcagc caggcgcagg
      841 atattccgct ccaccagggc gatacgggcg aggctccagt gcgcggcgtg cgcggcaatc
      901 tggcgatcca attcggggcc ggtctccacc gtcccgcgca ccaggctctc ggtgaacgcg
      961 cggacgtcct ccgggaccgg atggccctgc cagaagcttt ccagaatcgc cttcacctcg
     1021 cccggccgaa attccagctc gtagaggatc atcaaggcga gttcgcgtgc cttccgtcgt
     1081 ttgcccatga gcagcagcct tcagctatca gccgtcagca ttcagcgccc ggatcggctc
     1141 gtgactgact gctgatcgct gatcgctcac ttcagctccc gaaaaagatt caccatctcg
     1201 atggccgaac aggcggcatc gaagccttta ttgcccccct tggtgcccgc ccgctcgacg
     1261 g
//
LOCUS       DBNGZC010000272         7278 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_804776, whole genome shotgun sequence.
ACCESSION   DBNGZC010000272 DBNGZC010000000
VERSION     DBNGZC010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7278)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7278
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_804776"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="AC16G0_05015"
     CDS             <1..421
                     /locus_tag="AC16G0_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099018.1"
                     /translation="ALLPAGLALAFLDSLRALIVQGSFLRSLGDLILRHEAQPLGRRL
                     EDHFAAFATPHHLARLTRYRDLPLILFLARVVLAQDAKSLIRLAAVGVGREGLIRGLE
                     DRVRSRAAGVIRKLRAAVILLLAAILSIPQLTAWLLR"
     gene            538..1755
                     /locus_tag="AC16G0_05020"
     CDS             538..1755
                     /locus_tag="AC16G0_05020"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR5099019.1"
                     /translation="MTALRSLECTKCGVQHAVDRLMNLCPCGGILYPRYDLAALKGSY
                     DRTEVANGPKTLWRYRRVLPVRDDGNVISLGEGFTPIFQASRRGPFRAFPALYIKDES
                     PNPTQSFKARGMSPAISMAKELGAKEVALPSAGNAGSAAAAYSAKAGLTAYVFMPDDT
                     PSLIVSECIAYGARVYLVKGLINVCGRLVAAGVKARGWFDLSTLKEPYRVEGKKIMGY
                     EVAEQLGWTLPDWIFYPTGGGTGLVGMWKAFAEMEALGWIGQKRPRMVSVQAAGCQPI
                     VKTFTEGRSDSVLHENAHTVASGLRVPKALGDYLILQALRESGGLAVAVTDEELLRAQ
                     REVAEIQGILPCPEGGATWAAIQKLVAQGTVRPTETIVLFNTGSGLKYPECLPHEAPV
                     LDPDDPKVLQAVA"
     gene            1768..2031
                     /locus_tag="AC16G0_05025"
     CDS             1768..2031
                     /locus_tag="AC16G0_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099020.1"
                     /translation="MRPPRIPLVVECYSGYTADERPVAIQLGGRRILVQTVIDRWYGE
                     DHAYFKLAGEDGAVYLIRQDRTTDTWELILMESPPSPASQVKE"
     gene            2031..2738
                     /locus_tag="AC16G0_05030"
     CDS             2031..2738
                     /locus_tag="AC16G0_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099021.1"
                     /translation="MRRPRLFPLEDVLLAQLIPGFLFVMGWTTMYEVYHEEGSYFTTL
                     IQEILGSEGLFPSFLILAVLMSFPVGMAVDAVRHVVGEVWLKLPRTGWGKHRPSAFLE
                     WLEGKGDAIEEVEQRFSLYRHAQATLLTPARTAGNLGLVVLILTVWSSVKILRMGGVY
                     IFSLLFVVGVPLIGLGLGILLLVRYATGLGEFHREVHGSLFPSKAQFSTAPVTESPAF
                     PSDPSSLPTAPSPREGS"
     gene            3069..3719
                     /locus_tag="AC16G0_05035"
     CDS             3069..3719
                     /locus_tag="AC16G0_05035"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR5099022.1"
                     /translation="MRVIIVGCGRVGSNLANALSAERHEVVVIDRNPLSFGRLSREFT
                     GRMLTGVGFDREILQKADIEGVDALAATTDSDNVNIVVAVTAKETFKVPRVVARIYNP
                     QAAEIYRREGIPTVTPTLWGANAMRAMLLHPGVGTITTFGSGEVHVLEVEIPNSLAGR
                     PAEDLCAAGESLVGILIRRGKASIPSTDTRLESGDILRIAATAAAIPRLQKILAHS"
     gene            3733..4401
                     /locus_tag="AC16G0_05040"
     CDS             3733..4401
                     /locus_tag="AC16G0_05040"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR5099023.1"
                     /translation="MLAVVVGGGRGGSYLAQDLQAQGYQVKVVDRRPEVVARLRQEIE
                     GEVICGDGCSPEILEQVGTTKADLVVAMTHDDEDNLVVCRLAKHHFHVPRVITRVNNP
                     RNEWLYTKAWGVDVAISQVHLTAKVIEEEIGLGELVTLLKLNRGEAALVDLRLPETSP
                     AQGKAIRDLNLPANTVIVSVIREGKLVIPRGDTLLQAGDEILAVSAVAAEKSLKETLV
                     GPAA"
     gene            4449..6221
                     /locus_tag="AC16G0_05045"
     CDS             4449..6221
                     /locus_tag="AC16G0_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR5099024.1"
                     /translation="MSTAQARHERLGKIAALAIFASDMMSSVAYATEEILLVLVLGGS
                     AAMQYAPSIGIAIGLLAATVASSYWQTIHAYPSGGGAYVVANDNLGTYPGLIAGSALL
                     VDYVLTVSVSTASGVAAITSAFPALYPSRVAICVMFVFLVMVANLRGIRETGRIFTVP
                     TYWFILCLVLLVGGGFYHLLTQGPPPPPEAVPVQESLTIFLILRAFSGGCAALTGTEA
                     VANGVQYFRPPESRNAGITLIWMASILALSFLGVTFLAHFYHIVPRTEETVVSMLGRQ
                     LFGRGLIYYMIQTATAVILLLAVNTSFAGFPMLASIMAKDGFLPRQFANLGDRLVFSN
                     GIIILAGVSSLLLVVFGGSTHALIPLYAIGVFTAFTLSQAGMVKHWWREHGPRWQSHA
                     AINALGAITTGIVVAVIGTTKFVHGAWIVVSIVPMLVLAFLAVRRHYESLRGQLTLKG
                     FSPPKLGRHPVVVLVGGIHRGVVTALTYAKAISPNVTAISVDLDPTATSRLRQQWQEW
                     APDVPLVVLDSPYRSVMHPVLNYIDQMEKQRDGDYMTIILPEFVPAKWWHHLLHNQTA
                     LLLKTALLFRRGKVAISIPYHLDR"
     gene            7040..>7278
                     /locus_tag="AC16G0_05050"
     CDS             7040..>7278
                     /locus_tag="AC16G0_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099025.1"
                     /translation="METLPLYVLQEEAAPTLRTNRPKPSWWALYAVLPLTVLLFLLAD
                     QVPAMTGWRTVSELFASLVILGAIALWIHTNRVAL"
BASE COUNT         1240 a         2399 c         2209 g         1430 t
ORIGIN      
        1 cgcgctcctc cccgctggtc tcgccctggc cttcctggat tccctgcgcg ccctgatcgt
       61 tcagggttct ttcctgcgat ccctcgggga cctgatcctt cgccacgagg cccagcctct
      121 cggtcgacgg ctggaagatc atttcgctgc cttcgccact ccccaccatc tcgcgcgcct
      181 cacccgatat cgcgacctcc ccctcatcct gttcctggcc cgtgtggttc tggcgcagga
      241 cgcgaaatcg ctcatacggt tggccgccgt aggtgttggt cgggaagggc tcatccgggg
      301 actggaggat cgggtgcgca gccgggccgc cggcgtcatc cgcaagttac gggctgccgt
      361 gattctcctc ctcgccgcga tcctttccat cccccaactg accgcctggc tcctccgcta
      421 gccggcggcc cgccaggccg ctgcttccac cgcaggcagg gtctgcgact tgacagggcg
      481 atttgagcgg gggatagtac gagcggcaat accgatcacc tgaaggagga gagctctatg
      541 accgcgctcc gcagtctgga atgcaccaag tgcggcgtcc agcatgcagt cgatcgcctg
      601 atgaatctct gcccgtgcgg gggcatcctc tacccccgct atgacttggc cgccctgaag
      661 ggcagctacg atcggacgga ggtggcgaac ggccccaaga cgctgtggcg ctatcgtcgc
      721 gtcctaccgg tccgagacga cggcaacgtg atctccctgg gcgagggatt cacccccatc
      781 ttccaggcga gccggcgcgg accgttccgg gccttccctg ccctgtacat caaggacgaa
      841 agccccaatc ccacccagtc cttcaaggcg cggggcatgt ctcccgccat ctccatggca
      901 aaggagttgg gcgcaaagga ggtggctctc ccctccgccg ggaatgcagg cagcgccgcg
      961 gcggcctaca gcgccaaggc gggtctcacc gcctacgtct tcatgcccga cgacacccca
     1021 tccctcatcg tcagcgagtg catcgcttac ggtgctcgcg tctatctggt gaagggactg
     1081 atcaacgtct gcgggcgatt ggtggcggcc ggggtcaagg cgcgcggctg gttcgatctg
     1141 tccacgctga aggagccgta ccgcgtggag ggcaagaaga tcatggggta cgaggtggcc
     1201 gagcagctcg gctggacgct gcccgattgg atcttttacc ccacgggcgg agggacgggc
     1261 ctcgtgggaa tgtggaaggc cttcgccgag atggaggccc tgggatggat tggccagaag
     1321 cgaccgcgga tggtgtccgt ccaggccgcc ggctgtcagc ccatcgtcaa gacattcacc
     1381 gagggacggt cggattctgt cctgcacgag aacgcccata ccgtggccag cgggcttcgg
     1441 gtccccaagg ccctggggga ctacctcatc ctccaggccc tgcgcgagag cggaggcctg
     1501 gccgtggccg tgacggatga ggaactcctc cgagcccagc gcgaggtcgc tgaaatccaa
     1561 gggatcctgc cctgccccga gggaggcgca acctgggccg ccatccagaa gctggttgct
     1621 caaggaacgg tcaggccgac cgagacgatc gtcctcttca acacggggtc gggcttgaag
     1681 tatcccgagt gtctgccgca cgaagccccg gtcctggatc ccgacgaccc caaggtgctg
     1741 caggccgtgg cctgaggtga cgccgtgatg cgtccgccgc gcatcccgct cgtcgtggag
     1801 tgctactccg gctacaccgc ggacgagcgg ccggtagcga ttcagcttgg agggcgccgg
     1861 atcctcgtgc agaccgtcat cgatcgctgg tacggggagg atcacgcgta cttcaagctc
     1921 gccggggaag acggggcggt ctatctcatc cggcaggacc ggacgaccga cacctgggag
     1981 ttgatcctca tggaatcccc cccatccccc gcgagccagg tgaaagagtg atgcggcgac
     2041 ccaggctgtt ccccctcgaa gacgtgttgc tggcgcaact gatccccggc tttctgttcg
     2101 tcatgggctg gaccaccatg tacgaggtct accacgagga aggctcgtac ttcacgaccc
     2161 tgatccagga gatcctgggg agcgaggggc tcttcccctc cttcctcatc ctggccgttc
     2221 tcatgagctt cccggtcggg atggcagtgg atgcggtccg acacgtggtg ggcgaggtgt
     2281 ggctcaaact cccccggacg ggttggggaa agcacaggcc gtcggctttc ttggaatggc
     2341 tggaggggaa gggcgatgcc atcgaggagg tcgagcagcg attcagcctc tatcggcacg
     2401 cccaggccac cctgctcacc cccgccagga cggcggggaa cttggggttg gtggttctga
     2461 tcctcacggt ctggtcaagc gtgaagatcc ttcgcatggg aggcgtgtac atcttttccc
     2521 tgctgttcgt cgtcggggta ccgctcatcg gcttgggcct cggtatcctg cttctggtca
     2581 ggtacgcaac cggcttgggc gagtttcatc gcgaggtcca tgggtctctc tttccctcga
     2641 aggcacaatt ctccaccgcg ccagtaaccg agtctcctgc cttcccgtcc gacccttcca
     2701 gcctgccgac ggccccctcg ccccgcgaag gctcctgatt cggcatgttc ccggtgcccc
     2761 gcgcgccatg ccccgtcccc cgtgctccct accccgtgcc gcattccttc ttccggtcgc
     2821 ggtccgaagg tgccagactt ctgaccgccc gccgtctttg cttgacaagc ggagagcctc
     2881 ggccttagca ttcgtcccaa tctcagcagg atggcgagga aatttgcgcg tgtctgccat
     2941 tcagcggggg ggttgggtct tcggacccgg gagaacgtac atccgattgt gatggtccac
     3001 cgagccgcag taagagggtg ctgcggctcg tccgttttcg cctgccgttc cgtccaggag
     3061 gtcttggcat gcgggtcatc atcgtggggt gcgggcgggt cgggtcgaac ttggccaacg
     3121 ccctttcggc ggagcggcac gaggtggtgg tcatcgaccg gaaccccctg tctttcggcc
     3181 gactttcgcg ggagttcacc ggtcggatgc tcacgggcgt gggtttcgac cgggaaatcc
     3241 ttcagaaggc ggatatcgag ggagtcgacg cattggctgc taccacagac agtgacaatg
     3301 tcaacatcgt cgtggcggtg accgccaagg agacattcaa ggtcccgcgc gtggtggcac
     3361 gcatctataa tccccaggcc gccgagatct accgtcggga ggggattcca acggtcaccc
     3421 cgaccctctg gggcgccaac gccatgcggg cgatgcttct gcacccgggc gtgggcacca
     3481 ttaccacctt tggcagcggt gaagtccatg tcctcgaggt ggagattccg aactccctgg
     3541 cgggaagacc cgccgaggac ctctgcgcag ccggggaatc gctggtgggg atccttatac
     3601 gccggggaaa ggccagcatc ccgagtaccg acacccgtct cgaatccggc gacatcctac
     3661 gcatcgcagc gacggcagca gccatcccga ggctccaaaa aattctagcg cattcctgat
     3721 gggagggttc ccatgctcgc agtggttgtc ggcggcggcc gaggcggttc gtacctggcg
     3781 caagatctcc aggctcaagg atatcaggtc aaggtggtgg atcgccgtcc agaggtggtc
     3841 gccaggcttc gccaggagat tgaaggggag gtgatctgcg gagatgggtg tagtccggag
     3901 atcctggaac aggtcggcac gaccaaggcg gacctggtcg tggccatgac ccacgacgac
     3961 gaggacaacc tggtcgtctg tcgtctggcc aagcaccatt tccacgtccc ccgggtgatc
     4021 acccgcgtga acaacccgcg gaacgagtgg ctgtacacca aagcctgggg cgtggacgtg
     4081 gccatcagcc aggtccacct caccgccaag gtcatcgagg aggagatcgg cctcggcgag
     4141 ctggtcacct tgctcaaact caatcggggt gaggctgcct tggttgacct acgcctcccc
     4201 gagacgtccc ccgcccaggg aaaagccatc cgcgatctca atttgcctgc caacaccgtc
     4261 atcgtcagcg tgatccgcga gggaaagctc gtcatcccgc ggggcgatac cctgctacag
     4321 gccggcgatg aaatcctggc cgtgagcgcc gtcgcggcgg aaaaatccct gaaggagacg
     4381 cttgtcggtc ccgccgcgta ggaattagca tggccaccgc actgaaacgc cttctcctcg
     4441 gtaccccgat gagcactgcc caagcccgac acgagcgctt gggcaagatc gccgccctgg
     4501 cgatattcgc ctccgatatg atgtcctccg ttgcctacgc cacggaggag atcctgctcg
     4561 tcctcgtgct gggcggttcc gcggccatgc agtacgctcc gtcgatcgga atcgccatcg
     4621 gcctcctggc tgcaacggtg gcgagctcgt actggcagac aatccacgcc tacccttcag
     4681 gcggcggggc ctacgtcgtc gccaacgaca acctgggaac ctatcccgga ctcatcgccg
     4741 gttccgccct cttggtggat tatgtcctca cggtatccgt gagcaccgcc tcgggtgtgg
     4801 cggcgataac ttccgccttc cccgccctgt atccctctcg ggtcgccatc tgcgtcatgt
     4861 ttgtcttctt ggtgatggtt gccaacctgc gggggattcg tgagacgggt cgaatcttca
     4921 ccgtgcccac atactggttc atcctctgcc tcgtcctcct ggtgggcggg ggcttctacc
     4981 atctgctcac tcaggggccg ccgcctccgc cggaagcggt gcctgtgcag gaatctctga
     5041 ccattttcct catcctgcgc gccttctccg gggggtgcgc cgcgcttacc ggaacggagg
     5101 cggtcgccaa tggcgtccag tacttccgcc cgccggaatc ccgcaacgcc gggatcaccc
     5161 tcatctggat ggccagcatc ctggccttgt cattcctggg ggtcacgttt ctggcgcatt
     5221 tctaccatat cgtgcctcgg accgaggaga cagtcgtctc catgctggga cggcagctct
     5281 tcggccgggg attgatctac tacatgatcc agacagccac ggccgttatc ctgctcctgg
     5341 cggttaatac cagcttcgcc ggatttccca tgttggcctc gatcatggcc aaggatggat
     5401 tcctcccccg gcagttcgct aacctggggg accgcctggt cttcagtaac ggcatcatca
     5461 tcctggcagg cgtgtccagc ctgttgctgg tcgtcttcgg cggaagcact cacgcactga
     5521 tccctctcta cgccatcggc gtcttcaccg ccttcaccct ctcgcaggca gggatggtga
     5581 agcactggtg gagggagcac gggccccgct ggcaatcgca cgcggcgatc aatgccctcg
     5641 gtgcgatcac cacggggatc gtcgtggccg tcatcgggac gaccaagttc gtgcacggcg
     5701 cctggatcgt cgtcagtatt gttccgatgc tggtcctggc ctttctcgcg gtccggcgcc
     5761 attacgagtc cttgagaggc caactcacgc tgaagggatt ctctcccccc aagctaggga
     5821 gacatccggt ggtcgtcctc gtggggggca tccatcgcgg cgtcgtcacc gccctcacct
     5881 atgccaaggc catctcaccc aacgtgacgg ctatctccgt agacctcgat cccacagcaa
     5941 catcgcggct ccggcaacag tggcaggaat gggccccgga tgtcccgctg gttgtgctgg
     6001 actccccata ccgttccgtc atgcacccgg tcttgaatta catcgatcag atggagaagc
     6061 aacgggacgg ggattacatg acgatcatcc tgccggagtt cgtcccggcc aagtggtggc
     6121 accacctgct ccacaaccag acggccctgc ttctcaagac cgcgctcctc ttccggcgcg
     6181 ggaaggtggc catcagcatt ccttatcacc tcgaccgata ggccgcccga ttccccctgg
     6241 cgatccacgg accccgctga atctttatac aatccttata cattctagga gcttccttat
     6301 gccttcttta tttgaccggc tagaggagag ccagtagcct acaagcatag gagaaccaag
     6361 cggaacacct tctgacactc gtctcattca ccgccacgaa atccgcggtg aatgccgcgc
     6421 tgagggagag agggaacagc ccatgaagtc gagcaggcac gcgccagcac ccaaatggct
     6481 ttggctgtac gcgctgattc cgctgacgct cgggctcttc tggtttgccg acctcctgcc
     6541 cgagggtggt gtgtggcgag tcatgtcgga atgcggcgcg acattggcac ttttcggcct
     6601 cgccatcgtc tgggtccgcg ccaaccgaat tccgctgaca cagacgagtc tgggggcggt
     6661 cacggactcc ggtgtcccat caccggaaag catcccgaga ttctcggttc ggcggcgccg
     6721 gatctcccaa caggccgtcg ctgcgccccg agccccattg cgggcggtcc agtagccctc
     6781 taattacaaa ggtcgcagag tccaccggac accgaattct cccccttcca gctctgaaca
     6841 ccggaactct ccgctcttga tactcccttg atattcctca ctcaaatctt agcgacatcc
     6901 ttatttgacc gccaaagagg agcgccatat cttgccattg gagggagatt gtcagatgga
     6961 tacgatcttg agcggggcag tttcgggtgg ccaaggactt ctcacggacg gttcagatgc
     7021 acccattctc tcagggacca tggagacact gcccctatat gtgctgcaag aggaggccgc
     7081 cccaacgctc aggacgaata ggcccaagcc ttcctggtgg gccctttacg ccgtcctccc
     7141 cctcaccgtt ctcctctttc tcctggcgga tcaagtgccg gcgatgaccg gatggcgcac
     7201 ggtgtcggag cttttcgcct ccctcgtgat tcttggggcc atcgccctct ggatacacac
     7261 aaaccgcgtg gcgctgat
//
LOCUS       DBNGZC010000273         1181 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_804870, whole genome shotgun sequence.
ACCESSION   DBNGZC010000273 DBNGZC010000000
VERSION     DBNGZC010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_804870"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="AC16G0_05055"
     CDS             <1..802
                     /locus_tag="AC16G0_05055"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF040249.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR5099026.1"
                     /translation="QYLADGSWTKRLHPGWAAHAGVTAALLAQAGFTGPERVFEGTQG
                     FYHAFAGDYDEARMGHLLESLSKEWEVDRLTFKPYPCGIIAHPYMDCALRLRLQHEIR
                     PDQISEVHCLTAEGPVPRLWEPLEAKRRPPNGYAAKFSLPYLVAVMLAKARAGLAEFT
                     DEAVQDRTVLDLAAKVHYDLDPTIDYPRQFAGHVRISLKDGRVVEDRQDRPRGGPDLP
                     MTREELEAKFRGNAGLLMPLERIDEVVQAVGGMAAARRLDPLMTLLRT"
     gene            807..>1181
                     /locus_tag="AC16G0_05060"
     CDS             807..>1181
                     /locus_tag="AC16G0_05060"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5099027.1"
                     /translation="MELGLKGRVVLVTGASRGIGRAIALAFAAEGCRLALASRTPKEL
                     AAVGQEVESLGAEGRHWVADVTDPAQVEVLVEEVCRVWGEIDVLINNVGGGYPKNFEA
                     VSDEEWGQVVNLNLFSAIRLSRA"
BASE COUNT          217 a          356 c          398 g          210 t
ORIGIN      
        1 ccagtatctc gccgacggct catggacgaa gcggctccac cccggttggg ctgcccacgc
       61 cggcgtgacc gcggctctcc tcgcccaggc cggattcacc ggtcccgaga gagtcttcga
      121 ggggacacag ggcttctacc atgccttcgc gggcgactac gacgaggccc ggatgggtca
      181 tcttctggaa agcctgagca aggaatggga ggtggatcgc ctcacgttca agccataccc
      241 ttgcggcatt atcgcacacc cgtacatgga ctgcgccctt cgccttcggc tacagcatga
      301 gatccgaccg gatcagattt ccgaggttca ttgcctgacc gccgagggac ccgtaccgcg
      361 cctctgggag ccgctggagg caaagcggcg accgccaaac ggctacgcag ccaagttcag
      421 cttaccgtat cttgtggcgg tgatgctcgc caaggcaaga gctggtcttg ccgagttcac
      481 ggatgaggcc gtccaggatc ggaccgtcct ggacctagcc gccaaggtgc actacgatct
      541 ggatcccacc atagattacc cgaggcagtt cgcaggccat gtccggatca gcctcaagga
      601 cgggcgggtt gtcgaggatc gccaggatcg cccgcgaggc ggaccggatc ttcccatgac
      661 ccgagaagag ttggaggcca agtttcgtgg aaacgccggc ctcctcatgc ctttggagag
      721 aatcgacgag gtcgtccagg cggtgggcgg gatggctgcg gccagaaggc tcgacccact
      781 catgacgctt ctcaggacat aatctgatgg agctggggct gaaggggagg gtggtcctcg
      841 tcaccggggc cagccggggg atcggtcggg ccatcgccct ggcctttgcc gccgaggggt
      901 gcagactcgc cctggccagc cggacgccca aggaattggc ggcggtgggt caggaggtgg
      961 agtcccttgg cgccgagggg cgccactggg tagcggacgt caccgatccg gcccaggtcg
     1021 aggtgcttgt cgaggaagtc tgccgagtct ggggggagat cgatgtcctc atcaataatg
     1081 taggtggcgg ctatcccaag aattttgagg ccgtcagcga cgaggagtgg gggcaggtcg
     1141 tcaacttgaa ccttttctcc gcaatccgcc tgagccgtgc a
//
LOCUS       DBNGZC010000274         4349 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2173631, whole genome shotgun sequence.
ACCESSION   DBNGZC010000274 DBNGZC010000000
VERSION     DBNGZC010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2173631"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3885
                     /locus_tag="AC16G0_05065"
     CDS             <1..3885
                     /locus_tag="AC16G0_05065"
                     /inference="COORDINATES: protein motif:HMM:NF040138.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HIR5099028.1"
                     /translation="IGSGKSLAISASTTVAVTRTVTFSDQSQTPVAETPITTPGALGF
                     NATQSGNGLDGSAKSYDVIETPPPGFTPMVHGAVINHLPFGVAPVLLPVAGAIQQGVD
                     FGNTLVLGAELPDRTVGEGATVNIAPIYTVPVGTIDDYTFVWHVTSKNGQVVPDGSQS
                     RFSFVPVDEGIYDVTLSVTDTVRGRIASPYSFTVTAQNVAAAVNAGADQTAKEGQVWA
                     LAVPFADPGTQDTHTATINWGDGTATQAGVVTEASGAGSVSGTHVYADNGAYTITVTV
                     LDNDGASNSDSLVLSIGNAAPVVNAGPDLGAVEGSTFILTTASGFIDPGTRDSHTATI
                     NWGDGTSSAASVTESPFGPPGSASGMAGQVSGSHVYADNGTYVVTITVTDDDGGSSSG
                     SLRVVVGNAPPALQVGPDRTVAEGTKLELETLMPRWVPLPLPGHFELIRSGPAFTDPG
                     TRDTHTATVDWGDGTPSGTLTVTETPSGPPGSTGGMSGTLSGSHIYGDDGVYTVTITL
                     TDDDGGTVAGTLKVTVNNVAPVATADVYSVAQDGLLTVGAPAGVLATDSDVAGDPLLA
                     ELLTGPAHGSLTLNPDGGFTYLPAAHYTGPDQFTYLAHDDEGGASAPATVTLTVVPQT
                     APYVITGTQGADTLTLVQAGGTLTITLNGAVSTTPVSGLTHLEIYGLAGNDTITLTGL
                     TLPTMVDAGSGNDTVNATGVGAGVSLTLLGGAGHDTLTGGAGADVLVGGSGNDSLSGG
                     AGDDLFLVRTGEGRDVLDGGLGTDRLEVLGSVTAARITLGASGTHVSLGQTTPVPGLL
                     DLVNLESLSLTARGGADVIVVNALTGTTVQSVAIAAGGGNDLIDGSAVGVTVPLTLQG
                     GAGNDTLKGGPGADLLFGGTGNDSLQGGPGNDRLLGGEGADSLLGGSGDDRLLGGTGN
                     DTLDGNSGNDRLVGGDGDDIFVVQSGEGSDELRGGLGTDTLELTATVGADTITVSAIG
                     GHANILQTAPVSGSLDLIGIEYLHLTGHGGLDNITVRDLSRTDVLRVDLNGSPIYGPL
                     ALQAATPPTTVEASPVLTEAELEVIVEQAIAQWADTLVLPAATLDWLEQVQFQVTDLP
                     GLMLGEVIGSTVLIDQDAAGFGWFVDPTPRDNTEFVPADGELFASATTGALGHMDLLT
                     VVLHELGHLLGFLDLPPEDPGLMAETLEGGVRRLPSDAPPPASETLGSPTTLGAPAPE
                     VPAPRRDTEPKGLIDWNGLAQGALLGRQPLGLGTARRLTPAPEFVFAEEGRNGAEFTP
                     PSAGLTTDFAFEEMQDEPLLPEVEIPLDGEVG"
     gene            3899..4345
                     /locus_tag="AC16G0_05070"
     CDS             3899..4345
                     /locus_tag="AC16G0_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099029.1"
                     /translation="MSDGKGATVGSQPENPFTVWELMQRGNVSSTDQETRDNQRTGRS
                     RPGTHATAAQDGQEATMGRLVRTHWSPRSARALPALLLALLGGLLLPPPAHGATTYKV
                     LYAFPLTLPPLPSPVDPSGLFRDATGNLYGTTWFGGDNDQGAVSGR"
BASE COUNT          731 a         1489 c         1471 g          658 t
ORIGIN      
        1 atcggctccg gcaagtcgct cgccatctca gccagcacca ccgtcgccgt gacccgcacg
       61 gtgacattca gcgatcaatc gcagacgccg gtcgcagaaa ccccgatcac gacgcctggc
      121 gcgctcggct tcaacgccac gcagtccggc aacggcctcg acggcagtgc caagtcctac
      181 gacgtcatcg agaccccgcc gcctggcttt acgccaatgg tgcatggggc ggtgatcaac
      241 catcttccgt tcggcgtggc gccggtcttg ctcccggtgg cgggtgcgat ccagcaaggc
      301 gtcgattttg ggaacacgct ggtcctcggc gcggaattgc ccgacaggac cgtcggggaa
      361 ggcgcgacgg tcaacatcgc tcccatctac acggttccgg tcggcaccat cgacgactac
      421 acctttgtct ggcatgtgac gagcaagaac ggccaggtgg ttccggacgg cagccagtcg
      481 cgcttcagct tcgtgccggt ggacgagggc atctatgacg tcaccctgag cgtgaccgac
      541 acggtgaggg gacggatcgc ctctccgtac agcttcaccg tcacggccca gaatgtcgcg
      601 gcggcggtga atgccggcgc agaccagacg gccaaggagg gccaggtatg ggcactggca
      661 gtgcccttcg ccgatcccgg cacacaggat acgcacactg caaccatcaa ctggggtgac
      721 gggaccgcga cccaggccgg ggtggtgact gaggcgagcg gcgcgggttc agtctcgggc
      781 acgcatgtct atgcagacaa cggggcatat acgatcaccg tgaccgtcct ggacaacgac
      841 ggggctagca actcggatag cctggtcctg agcatcggca acgcggcccc ggtggtgaat
      901 gccggtcccg atctgggggc ggtcgagggc agcacgttca ttctcaccac cgcctcgggg
      961 ttcatcgatc ctggtacccg cgacagccat accgcgacca tcaactgggg cgacgggacc
     1021 agcagtgcgg cgagcgtcac cgaatcgccc ttcgggcctc cgggatcggc gtccggcatg
     1081 gcagggcagg tctccggcag ccacgtgtat gcggacaatg gcacgtacgt cgtgacgatc
     1141 actgttaccg acgatgacgg cggctcgagc tctggcagct tgagggtggt ggtcggaaac
     1201 gccccgcccg ctctccaggt cgggcctgat cgcacggtgg cggaagggac aaaactcgag
     1261 ctcgaaacac tcatgccgag atgggttccg ttgccgctcc cgggtcactt cgagttgatt
     1321 cggagcggcc ccgccttcac tgatccgggc acgcgggaca cccacacggc gaccgtggat
     1381 tggggcgacg ggacgcccag cggcaccctc accgtcacag agacgccatc aggacctccg
     1441 gggtcgacgg gcgggatgag cggcacgctg tcgggaagcc acatctatgg tgacgacggc
     1501 gtctacaccg tcaccatcac cctgacggat gacgatggcg gcacggtggc tgggacgctg
     1561 aaggtgaccg tcaacaacgt cgcccctgtg gccaccgcgg acgtgtactc ggtggcccag
     1621 gatgggctgc tgaccgtggg ggcccccgcc ggggtgctcg ccaccgactc ggatgtggcg
     1681 ggcgatccgc tcctcgccga gttgctcacc gggcccgccc atggcagcct gacccttaac
     1741 ccagacggcg gcttcaccta cctcccggcg gcgcattaca ccgggcccga tcagttcacc
     1801 tacctggccc acgacgacga gggaggggct tccgcgccgg ccacggtgac gcttacggtg
     1861 gtcccgcaga ccgccccata cgtcatcacg gggacccaag gcgccgacac cctcaccctg
     1921 gtgcaagcgg gcggaaccct caccatcacg ctgaacggcg ctgtctcgac gaccccggtg
     1981 agcggcctca cgcacctcga aatctatggc ctcgccggga acgacaccat taccctcacc
     2041 ggcctgaccc tccccaccat ggtggatgcg ggcagcggca acgacaccgt gaacgccacc
     2101 ggggtcgggg cgggcgtgtc cctcaccctc ctggggggcg ccggccatga caccctcacc
     2161 gggggtgccg gtgccgatgt cctcgtgggc gggagcggca atgactccct gagcggcggg
     2221 gccggggacg atctcttcct ggtacgcacg ggcgagggcc gggatgtgct cgacggcggg
     2281 ctcggcactg atcgcctgga ggtgctgggc tcggtcaccg cggcccggat caccctgggt
     2341 gccagcggca cccacgtcag cctggggcag acgacccccg tgcccggcct gctagacctc
     2401 gtgaacctcg agtccctctc cctcaccgcc cgcgggggcg ccgatgtaat cgtcgtgaac
     2461 gccctgaccg gcaccaccgt gcagtcggtg gcgattgcgg ccgggggtgg caacgacctc
     2521 atcgacggct cggccgtagg cgtgaccgtg cccctgaccc tccagggggg tgccggcaac
     2581 gataccctca agggcggccc gggggccgac ctcctgttcg gcgggaccgg caacgatagc
     2641 ctccagggcg gtcccgggaa cgaccgcctc ctcggcggcg agggggctga ctccctcctt
     2701 ggcggcagcg gggacgaccg cctgctgggc gggacgggga acgatacgct cgacgggaat
     2761 agcgggaatg accgcctcgt cggcggcgac ggggacgaca tctttgtggt tcaaagcggg
     2821 gagggcagcg acgagctccg cggcgggctc ggaaccgata ccctggagct gaccgcgacg
     2881 gtgggcgccg acacgatcac cgtgagcgcc atcggcggcc atgccaacat cctccagaca
     2941 gccccggtct ccggcagcct ggatctcatc ggcatcgagt acctgcacct caccggccac
     3001 ggaggtctgg acaatatcac ggtgcgcgac ctgagccgca cagatgtgct acgtgtggat
     3061 ttgaatggca gcccgatcta cggacctctc gctctccagg cggcgacccc gcccacgacg
     3121 gtcgaggcga gccccgtgct cactgaggcc gagctggagg tcatcgttga gcaagcgatt
     3181 gcccagtggg ccgacactct tgtgctcccg gcggccacgc tggactggct ggagcaagtt
     3241 cagttccagg tcaccgatct gcctggcctc atgctggggg aggtgatcgg gagcacggtc
     3301 ctgatcgatc aggatgctgc cggcttcggc tggttcgtgg atcccactcc gagggacaat
     3361 acggaattcg tccccgccga cggagagctc ttcgccagcg ccaccacagg tgccctgggc
     3421 cacatggacc ttttgaccgt ggtcctgcac gaactcgggc acctcctggg cttcctggat
     3481 ctgcccccgg aggaccccgg cctgatggcg gagactttgg agggaggggt gcggcggttg
     3541 ccctccgatg ctccaccgcc ggcctccgag accctcggga gcccgaccac gctaggtgct
     3601 ccggcgccag aagtgcctgc cccccgtcgg gacaccgagc cgaagggctt gattgattgg
     3661 aatggcctgg cccagggggc cctcctgggg cggcagccgc tgggactggg gactgctcgg
     3721 cggctcacgc cggccccgga gttcgtcttt gcggaggagg gccggaacgg agcggagttc
     3781 accccgccct cagccggcct gaccaccgac ttcgctttcg aggagatgca ggacgagccc
     3841 ctactcccgg aggtcgagat ccccctggac ggggaggtcg gctgagcgtg gccccagggt
     3901 gagtgacggg aagggagcaa ccgtgggcag ccagccggag aaccccttca cggtgtggga
     3961 gttgatgcag agaggcaacg tgagcagcac ggaccaggag acacgcgaca accagcgcac
     4021 cgggcgctcg cgtcccggca cgcatgcaac ggcagcccag gacgggcagg aggcaaccat
     4081 gggtaggctc gtgaggacac actggtcccc tcgctccgcc agggcgctcc ccgcgctgct
     4141 cctggccctc ctcggaggac tgctcctgcc gccacctgcg catggggcca ccacctacaa
     4201 ggtactctac gcctttccgc tgacgctccc gcccttgccg tcgccggtgg acccgagcgg
     4261 cctcttccgg gatgctaccg gaaaccttta cggcacgaca tggtttggtg gcgataacga
     4321 ccaaggggcc gtatcaggtc gataatgag
//
LOCUS       DBNGZC010000275         2621 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_217843, whole genome shotgun sequence.
ACCESSION   DBNGZC010000275 DBNGZC010000000
VERSION     DBNGZC010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_217843"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="AC16G0_05075"
     CDS             <1..266
                     /locus_tag="AC16G0_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012508619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099030.1"
                     /translation="PPPPPPPAAGGVDTLVFAGGIGERSAPIRARICAGLEFLGVHVD
                     SARNAAHSPVISPDDGTVTVRVIPTDEETTIARETLAIVGGAV"
     gene            389..>2621
                     /locus_tag="AC16G0_05080"
     CDS             389..>2621
                     /locus_tag="AC16G0_05080"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HIR5099031.1"
                     /translation="MTTLATRTAAVDPDRSISAYGPARSTIADSPLSAEELRKIDAYW
                     RASLYLCLGMLYLKDNPLLREPLKLAHTKPRLLGHWGSDAGQSFTYLHFNRLINKYDL
                     NAIFISGPGHGAPGLLSHAYLEGAYSEVYPDKTEDLVGMQRFFKQFSFPGGIGSHATP
                     ETPGSIHEGGELGYSVSHAYGTVYDNPDLITLVMVGDGEAETGPLATSWHSNKFLNPI
                     TDGAVLPVLHLNGYKINNPTILARISHDELEALFRGYGYTPYFVEGSDAPSMHQAMAA
                     TLEHCVLEIRKIQEEARRTGKANRPRWPMIILRSPKGWTAPRKVDGHYLEGFWRAHQV
                     PIADIGTNPGHLQVLEEWLRSYKPEELFGREGRLIPELRALAPKGNRRMSANPVANGG
                     LLRKPLDMPDFRSFAVDVRKAGATLAGSVPTLGNFLREVMRKNMQSFRVVGPDETQSN
                     QLQAIYEVAKKAWLGEYFEEDADGGELSPNGRVMEMLSEHTVEGWLEGYLLSGRHGFI
                     NSYEPFIHVIDSMFNQHAKWLEKCNELPWRAKVASLNLLITGLVWRQDHNGFTHQDPG
                     FLDVVANKSPDVVRIYLPPDANSLLSVADHCLRSVNYVNVIVADKKEHPQYLDMDAAI
                     AHCTKGAGIWNWASNDQGAEPDVVMASCGDVPTLEALAATALLRQHLPDVRVRFVNVL
                     DLFKLVPHSEHPHGLTDREFEALFTPDRPVIFNFHGYPWLIHRLTYRRPGQHHIHVRG
                     YKEQ"
BASE COUNT          506 a          870 c          829 g          416 t
ORIGIN      
        1 cccccccccc cccccccccc cccgcggccg ggggggtgga taccctcgtc tttgccgggg
       61 ggatcggcga gaggtcggct ccgatccgag ctcggatctg cgccgggttg gaattcctgg
      121 gagtccacgt cgactcggct cgtaacgccg cgcacagccc ggtgatctcg ccagacgacg
      181 gcaccgtgac cgtccgcgtg atcccgaccg atgaggaaac caccattgcc agggagacgc
      241 tggccatagt cggcggcgct gtctgaggaa atactcatgg aggaaaagga gtgtaagaag
      301 acgccccctg gcatagcgcc ataaggcgtc ggaaacgtgg gaagtctcgc ggggacacgg
      361 aacgtccccg ggaaaggaag gcatagtcat gaccactctc gccacacgga cagcggcagt
      421 tgatcccgac aggagcatca gcgcgtacgg cccggctcgc tcgacgatcg cggattcgcc
      481 cctcagcgcc gaggagttgc gcaagatcga cgcgtactgg cgtgccagcc tgtatctgtg
      541 cctggggatg ctctacctca aggacaaccc cttgctccgg gagccgttga aattggcgca
      601 cacgaagccg cggctgctgg gccactgggg gtcggatgcc ggccagtcgt tcacctacct
      661 ccacttcaac cggctgatca acaagtacga cctgaacgcc atctttatct ccggccccgg
      721 ccacggggcg ccggggctcc tgtcccacgc gtacttggag ggcgcctata gcgaggtgta
      781 tccggataag acggaagatc tcgtcggcat gcagcgcttc ttcaagcagt tctccttccc
      841 cggcggcatc ggcagccatg ccaccccgga gacccccggc agtatccacg agggcggcga
      901 actcggctac agcgtctccc acgcctacgg gacggtgtac gacaacccgg acctcatcac
      961 cttggtgatg gtgggcgacg gcgaggccga gacgggtccc ctggccacga gctggcatag
     1021 caacaagttc ctcaacccga tcaccgacgg cgcggtgctg cccgtcctac acctcaacgg
     1081 ctacaagatc aacaacccca ccattctggc ccgcatcagc cacgacgagt tggaggcgct
     1141 gttccgcggc tatggctaca ccccctactt tgtggagggg agtgatgcgc ccagcatgca
     1201 ccaggcgatg gccgcgaccc tggagcactg cgtcctggag atccggaaaa tccaggagga
     1261 agcgcggcgg accggcaagg ccaaccggcc gcgctggccc atgatcatcc tccgcagccc
     1321 gaagggctgg acggccccgc ggaaggtgga cgggcactac ctggaaggct tttggcgggc
     1381 gcaccaggtc ccgatcgcag acatcggtac caaccccggc cacttgcagg tgctggaaga
     1441 atggttgcgg agctacaagc cggaggaact gttcggtagg gagggacggc tgattcccga
     1501 gctgagggcc ttggccccca agggaaaccg gcgcatgagc gcgaatccgg tggccaatgg
     1561 cggtctgctg cgcaagccgc tggatatgcc ggatttccgc agcttcgccg tcgatgttag
     1621 gaaagcgggt gcaactctcg cggggagcgt ccccaccctc ggcaacttcc tccgcgaagt
     1681 catgcggaag aacatgcaga gcttccgcgt cgtcggcccg gacgagacgc agtccaacca
     1741 actccaggcc atctatgaag tcgccaagaa ggcctggctg ggggagtatt tcgaggagga
     1801 cgcggatggc ggcgaactgt cgccaaatgg ccgggtcatg gagatgctca gcgagcacac
     1861 cgtggaagga tggttggaag gctacctcct gagcggccgg cacggcttca tcaacagcta
     1921 cgagccgttc atccacgtca tcgactcgat gttcaaccag cacgccaagt ggctggagaa
     1981 gtgtaacgag ctgccctggc gggcgaaggt agcttccctt aacctcctca tcaccgggct
     2041 ggtctggcgg caggaccaca acgggttcac gcaccaggac cccgggttcc tcgacgtggt
     2101 cgccaacaag agtcccgacg tggtgcgcat ctacctgccg cccgatgcca actctctctt
     2161 gtcggtcgcg gaccactgcc tgagaagcgt caactacgtg aatgtcatcg tcgcggacaa
     2221 gaaggagcat ccgcaatacc tggacatgga cgcggcgatc gcccactgca cgaagggggc
     2281 cggcatctgg aactgggcca gcaacgacca gggcgccgag cccgatgtgg tcatggccag
     2341 ctgtggcgac gtgccgactc tcgaggccct ggcggccact gccctgctcc gccagcacct
     2401 gcccgacgtg cgggtccggt tcgtgaacgt gctggacctg ttcaagctcg tgccgcacag
     2461 cgagcacccg cacgggctga ccgaccgcga gttcgaggcc ctcttcaccc cggaccggcc
     2521 ggtcattttc aatttccacg gttacccttg gctgatccac cggttgacct accgccggcc
     2581 cggccagcac cacatccacg tgcgcggcta caaggagcag g
//
LOCUS       DBNGZC010000276         2048 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1001071, whole genome shotgun sequence.
ACCESSION   DBNGZC010000276 DBNGZC010000000
VERSION     DBNGZC010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1001071"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /locus_tag="AC16G0_05085"
     CDS             <1..1031
                     /locus_tag="AC16G0_05085"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR5099032.1"
                     /translation="PYHLVPEIQPILHRVLQDGTAAYTDIYHNEHGTWITAFAPVKNA
                     AGRTVAALDVDFRADVYLADLAAVRRRLYLHSLTAALLALGAGIFLARRITRPLAQLA
                     SVARGVVEGDLTTRVHITARDEIGMLGNVFFLMVQRLRVSYRSLTDVLVRALEARGGE
                     TGTLSRLTKAAAALAEKLDISPTQREALELGTLLHDIGETRTPEAILQKRGLLTAEER
                     RLIEQHPGAGVDILEAVPLLTPALDVVGGHHERYDGRGYPQGLRGEEIPLTARIFAVV
                     DALDAMTHDRPYRTGRSVSEAIEEIRREAGKQFDPRVVEAALTISEERWAELLAARPP
                     VPEPAHVS"
     gene            1078..1605
                     /locus_tag="AC16G0_05090"
     CDS             1078..1605
                     /locus_tag="AC16G0_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TQO small subunit DoxD"
                     /protein_id="HIR5099033.1"
                     /translation="MNSERSWATPLSPYVLGVLRISYGILWLQQAMWKIPPDFGMSHG
                     DGLFYWTKEMAKYSFLPPHKFFVEAVVLPHFLLFAWATLLTELFIAFSHLLGVASRLG
                     ALAALAMSTNLLIGLARQPSEWPWSYLMLVGYALVFLSTHPGRVLGLDGRLSEYFSTP
                     TRVNRPWASALRYLM"
     gene            1755..>2048
                     /locus_tag="AC16G0_05095"
     CDS             1755..>2048
                     /locus_tag="AC16G0_05095"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5099034.1"
                     /translation="MAYSVGLGLTNECNLSCAHCYRPTDGVHRLSLDDVRRICDSLPV
                     GSVNLGTGENGLHPDFNAITEYLVERGVRTSLTTNGHTVEVMPDGLVRSLAEVE"
BASE COUNT          343 a          670 c          647 g          388 t
ORIGIN      
        1 agccctatca cctcgtgccg gagatccagc ccatcctgca ccgcgtgctg caggacggca
       61 ccgccgccta caccgacatc tatcacaacg agcacgggac ctggatcacg gcttttgccc
      121 ccgtcaagaa cgctgctggc cgtaccgttg ccgcactgga tgtagacttc cgggcagatg
      181 tgtacctcgc agacctggca gcggtgcgac gacggctcta tcttcactcc ctgactgccg
      241 cattgctggc cctgggggcg gggattttcc tcgcccgccg gatcacccgg cccttggcgc
      301 aactcgccag cgtagcgcgg ggtgtggtag agggggacct cacgacccgg gttcacatta
      361 ccgctcggga cgagatcggc atgctgggga acgtattctt tctcatggtc caacgcctgc
      421 gcgtctcata ccggagcctg acggacgtgc tggtccgggc cctggaggca cgcgggggcg
      481 agaccgggac tctctcgcgg ttgacgaagg ccgcagccgc cctggcggaa aaattggaca
      541 tctcaccgac tcagcgcgag gccctggaac tggggacgct cctccacgac atcggcgaga
      601 cccggacccc ggaggcaatt ctccagaagc gcggtctcct gacggccgag gagcgacgat
      661 tgatcgagca gcatccgggg gcgggagtgg acatcctgga ggcggttcct ctcctcacgc
      721 cggccctgga tgtggtcggg ggtcatcacg agcgctacga tggtcgaggc tatccacaag
      781 ggttgcgagg cgaggagatt ccactcacgg ccaggatctt cgccgtggtc gacgccctgg
      841 acgccatgac ccacgaccgg ccttaccgga ccgggcggtc ggtgtccgag gcaattgagg
      901 agatccgacg cgaagcgggg aagcagtttg acccgcgggt ggtcgaggcg gcattgacca
      961 tttcggaaga gcggtgggca gagctattgg ccgcgcgtcc acccgttccg gagccggcgc
     1021 acgtttcgtg acatcctgag acttcgaaag atctttgacg atctggagga aacgagcatg
     1081 aatagcgagc gaagctgggc aacccccctg agcccgtatg tccttggggt cctccgcatc
     1141 tcctacggca tcctctggct gcaacaggcc atgtggaaga taccgccgga cttcggcatg
     1201 tcccatggcg atgggctgtt ctactggacc aaggagatgg cgaagtactc cttcctgccg
     1261 ccgcataagt tcttcgtgga ggccgtggtc ctcccgcact tcctgctgtt cgcgtgggca
     1321 actctcctca ccgaattgtt catcgccttc tcgcatctcc tgggggtcgc cagccgtctg
     1381 ggggccctgg cggccctggc gatgtccaca aacctgctca tcgggctggc ccggcagccc
     1441 agcgagtggc cgtggagcta cctcatgctg gtaggctacg ccctcgtctt tctgagcact
     1501 cacccgggcc gggtcttggg actggatggg cggctctccg aatacttcag cacgccgacg
     1561 cgtgtgaacc ggccgtgggc cagtgccctg cggtatctga tgtagccacc acgccttgag
     1621 cagacgcgtg gattgctgtt gttcttcccc ggttgcctcg tgaccatacc tcggttcatt
     1681 ttcgcgcctc gggccttgca tccggcttct cgagccaggt gcccggtttg tccccagagg
     1741 ctgagatctg cccaatggca tactccgtcg gacttggcct cacgaatgag tgcaatctct
     1801 cgtgcgccca ctgctaccgg cctaccgacg gagttcatcg cctctccctg gacgacgtcc
     1861 gccggatctg cgacagcctc ccggtgggct cggtcaacct gggaaccggc gagaacggtc
     1921 tgcacccgga cttcaatgcg atcaccgaat acctcgtgga acgaggcgtc cggaccagtc
     1981 tcacgaccaa cggccacacg gtcgaggtga tgcccgatgg cctcgtgcga tcgctcgccg
     2041 aggtggag
//
LOCUS       DBNGZC010000277         2476 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1978824, whole genome shotgun sequence.
ACCESSION   DBNGZC010000277 DBNGZC010000000
VERSION     DBNGZC010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1978824"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..559
                     /locus_tag="AC16G0_05100"
     CDS             74..559
                     /locus_tag="AC16G0_05100"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR5099035.1"
                     /translation="MRIILEQQVSLASARSIFRRLTTKVAPFSAIRFIELGESYFRSL
                     GITRQKAAYCLHVARAVAEGHLRFAAVARASDSAARAALTQIKGIGPWTADIYLLMAL
                     RRADVWPSGDLALVKAASKLKKLRGHPTPIAIAAIAEGWRPFRSVAARMLWQYYLAGD
                     G"
     gene            867..1607
                     /locus_tag="AC16G0_05105"
     CDS             867..1607
                     /locus_tag="AC16G0_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099036.1"
                     /translation="MPRMMRRSADLWLLALLVIGCSHAPLPIPVQSIESYPFRQTQAG
                     VRVAVDPYFTQDRLQVAFSGGDEFSQNGLLPVRVILENGSPGALQVDPRAFRLVRPDG
                     SMELALSPEDALSLIKKSMGWWAIGGGLIAGPAAAYQNEGRQKSLDARALKGDTLREG
                     GSGSGFVYFGITDKDKNLAGFRIVFALVGPGDKALTFEVPIDGRRDIPVPSGRAETAE
                     QSRPPVQAPIDPRNPQSPTRIEGYGGQE"
     gene            complement(1975..>2476)
                     /locus_tag="AC16G0_05110"
     CDS             complement(1975..>2476)
                     /locus_tag="AC16G0_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099037.1"
                     /translation="FAGIVDALRYGQREGLLRLDRGRQGGVRVHPGPQFLTLTKSSEA
                     VPAADLPASGESPQPVLLPQDVAAIPVQDAHLDADQPLTTQESLPFEAATAPAAQEAE
                     AESPAPSSEVTSAEGAGSPAEPATPPPAAGRKRTGARTGTRAKKTTPATGGVKKKAVR
                     PRAKKS"
BASE COUNT          449 a          758 c          826 g          443 t
ORIGIN      
        1 gtaatgggcg gcgatcctgg atcagtacgg accgcccccg ctgtgggggc ggacgcctgg
       61 gttcccgacc ctggtgcgga ttatcctgga acagcaggtc tcactggctt cggctcgctc
      121 gatcttccga cgcctgacga cgaaggtggc cccgttctcg gcgattcggt tcatcgaatt
      181 gggcgagtcg tatttcagat cgctgggaat cacccggcag aaggccgctt attgcctgca
      241 cgtggctcgg gcggtggctg aaggacatct caggttcgcc gccgtggcgc gcgcgagtga
      301 ttctgctgcc agggccgcac tgacgcagat caaaggtatc ggaccctgga cggcggacat
      361 ctatctcctg atggcgctcc gccgggcgga cgtgtggccc tcaggcgacc tggctcttgt
      421 gaaggccgcc agcaagctaa aaaaacttcg agggcaccca acaccgatcg cgattgcagc
      481 catcgccgaa ggctggcggc cgttccgatc ggtcgccgct aggatgctct ggcaatatta
      541 cctggccggg gacggatgaa aatccgaatc gggattggca ccgaggcggc ggtcgggctt
      601 tcattcggcc ggcacgtcca agcctcccgt gatatgctcc tgaccgcatt ccgaaatacc
      661 aggcgtcagt gacgtccggc gccaccggac gcccggaacg ctgagatcga aatgacgcgg
      721 cgggggggag acgattggat gcttgaaagc ccgagacgtc actgcgatta tgactgcaag
      781 ggccgggata ccgtctcggc gtccgccgaa ggcgaatcgc ggtcatgacg gtttccaggg
      841 tcttccgtcc ggcaaggagg gtgagtgtgc cacgaatgat gcgacgttcg gctgatcttt
      901 ggctgctggc gctgcttgtg atcggttgct cccacgcccc tctgccgatc ccggtgcagt
      961 cgatcgagag ctatccgttt cggcagacgc aggcaggcgt ccgggtcgcc gtggatccat
     1021 acttcaccca ggatcgcctg caggtagcct tcagcggggg cgacgagttc tcccaaaacg
     1081 ggctgctgcc ggtccgggtc atcttggaaa atgggagtcc gggtgcactc caggttgatc
     1141 cgcgggcatt tcgcctggtc cgtcccgacg gctcgatgga attggccctc tcgcccgaag
     1201 acgcgctctc cctgatcaag aaatccatgg gctggtgggc catagggggc gggttgatcg
     1261 cgggaccggc cgcagcttac cagaatgagg gccgacaaaa atccctggac gctcgcgccc
     1321 tgaaaggaga caccctccga gagggaggct cgggaagcgg attcgtttat ttcggcatca
     1381 cggacaaaga caagaacctg gcgggattcc gcatcgtgtt cgcgctggtt ggaccggggg
     1441 acaaggcttt gaccttcgag gttcccattg acggacgccg agacatccct gtgccaagcg
     1501 ggagggctga gacggcggag caatccagac ctcccgtgca ggcacccatc gatcccagga
     1561 atccgcagag ccccacgcgc atcgaggggt acggcgggca ggagtgatca ttcgaagccc
     1621 gacacaataa gaacagttac cggctgccgg tcatcggccg tgataggcaa aggctgggcg
     1681 cggccgagaa attcgaagct cggagttcga atcttggaat ggttcatccc cagtcttcga
     1741 ctcccaccct cgaaccgccg ccccgtatag ccaacggctg aaggcaagga aggaagaacg
     1801 ggatgccaac ggatgcattg atccacccca acgcccaagg cagtcgcgag gcgggagtca
     1861 tcgtctccgt caacttcgag cgcgcaggct agttagaagt acatcagctt cgaagtccgc
     1921 agtctggggg agggcgagaa gctctcgtcg cagggcgaga tcggcgtctc gagtctacga
     1981 cttcttcgcg cggggacgca cggccttctt cttgactcca ccggttgccg gcgtggtctt
     2041 cttcgcgcgc gtgcccgtgc gtgcacccgt gcgcttgcga cccgcggcag gcggcggggt
     2101 cgcgggctcg gctggggagc ctgccccttc ggccgacgtg acctcactcg agggcgcggg
     2161 tgactccgcc tcagcctcct gagcagctgg cgccgtggcc gcctcgaagg gcagcgactc
     2221 ctgtgtcgtc agcggttgat ccgcatcgag atgggcgtcc tggacgggaa tggccgcgac
     2281 gtcctgtggc aagagaaccg gctgcgggct ctcgccgctg gcgggaaggt cagctgcggg
     2341 tacagcctca gaggacttcg tcagcgtcag gaattgcggt ccgggatgca cgcgcacgcc
     2401 gccctgcctg ccgcgatcaa gtcggagcag gccttcacgc tgtccataac gcaaggcatc
     2461 cacgatgccg gcgaat
//
LOCUS       DBNGZC010000278         4399 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1586694, whole genome shotgun sequence.
ACCESSION   DBNGZC010000278 DBNGZC010000000
VERSION     DBNGZC010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1586694"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="AC16G0_05115"
     CDS             complement(<1..983)
                     /locus_tag="AC16G0_05115"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR5099038.1"
                     /translation="MLDTRNVTCGMLKDLCGGLRGGWRGLILIVLCAVLLLEAPAVGA
                     RRRAAPKGGQHPPKPARVAGPATPSWIVINAATGDVLTSQDPDRPGAPASMTKVMLAL
                     LVMEAVRDGQLKLSDTVQTSRLASKMGGSQVYLKEGETFPVEDMMAALLIGSANDAAT
                     ALAERLTGSLEATIARMNERAAALKLTRTRFGSVHGLPPGPGQEGDVTTPRDMARLAQ
                     ELIKFPDVLRWTSSPEAPFRGGTFILRNSNHLIGKFPGADGLKTGHFHEAGYNLAATA
                     KRGELRLIAVVMGAPTNAARFAEAARLLGEGFGRYVEVTVVKAGAPLGGEIR"
     gene            1298..2248
                     /locus_tag="AC16G0_05120"
     CDS             1298..2248
                     /locus_tag="AC16G0_05120"
                     /inference="COORDINATES: protein motif:HMM:NF018968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1464 family protein"
                     /protein_id="HIR5099039.1"
                     /translation="MLSLGIDYEAGQWKLAAWNEDRAADLLRFGSEGDVWESVEDILS
                     AHPATPIVLPSGFGIPVTRARELLDRDIAEITLHRKAHVTDPLGQFLAEARRRPLRAF
                     CIPSVRLLPSIPLHRKLNQVDLGTADVLCAAAWAIHCLAQGGPTSATSHFLLLHVGAD
                     TRALVAIRDGRIVDGIGRSAWGMGTASRDSADGFLTSLGQGIGRSHAAGGTEKLENRL
                     REARNHAFWETVEKESVSLLAFHGLSQVVVTGPGSAQALQAAGDRLPITPLPSQVDGY
                     EAALGAALIAAGLTGGPTANLVDHLELRQVRERVFDWIVP"
     gene            complement(2395..3750)
                     /locus_tag="AC16G0_05125"
     CDS             complement(2395..3750)
                     /locus_tag="AC16G0_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR5099040.1"
                     /translation="MRSTLAALEAVAAAQAIRRGDISSEQLVRACLERIAEVEPVVQA
                     WAFLDAEYALAQARAADRAQAQGVPLGPLHGVPVGVKDIFDTADMPTENGTVLHAGRR
                     PSEDATVVSLLRAAGAVVLGKTVTTELAVYFPGKTTNPHDRRYTPGGSSSGSAAAVAA
                     GMIPLAVGSQTNGSVIRPAAYCGVYGFKPSYGLVSRHGVLAQSWHLDHVGVFARTLAD
                     VAYLAAELMAFDSRDAAMRPQARPRLADVMPQAPPVQPCLAFVKSPFWDEAAEDTQAE
                     FARLTARLGTQVNEVDLPPAFAKAAEVHRTILEADLARSFAAEYERGAGQLSARLREM
                     IERGQRTLATEYNRAVAAIPNLARDLDPIFARFDAILTPAATSQAPLGLESTGSPIFC
                     TIWTLCGLPAVTLPILRGAAGLPMGAQVVGKKLGDAPLLRTARWLATAAADSDRPAAH
                     G"
     gene            complement(3841..3917)
                     /locus_tag="AC16G0_05130"
     tRNA            complement(3841..3917)
                     /locus_tag="AC16G0_05130"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3881..3883),aa:Val,seq:cac)
     gene            complement(4032..>4399)
                     /locus_tag="AC16G0_05135"
     CDS             complement(4032..>4399)
                     /locus_tag="AC16G0_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3417 domain-containing protein"
                     /protein_id="HIR5099041.1"
                     /translation="AWKERVRARWREVAVQAVTADMLTPITAGATREVWVEVALGSLQ
                     PEDVSVALYAGPLTGEGEITTATAEAMKVEGVIRPGVYVYSGALPAPSTGQHGFRVRI
                     LPAHEDLAAPYSLNCIVWG"
BASE COUNT          727 a         1544 c         1412 g          716 t
ORIGIN      
        1 cggatttccc cgcccagcgg cgcgccggcc ttcaccacgg tgacctcgac gtaccggccg
       61 aatccttcgc ccagcagccg cgccgcctcg gcgaagcgcg cggcgttcgt gggtgcgccc
      121 atgaccacgg caatgagccg cagttctccg cgcttggcgg tggcggcgag gttgtacccg
      181 gcctcatgga agtgaccagt cttcaagccg tcggcccccg gaaacttgcc gatcaggtga
      241 ttgctgttcc gcaggatgaa cgtcccaccg cgaaagggag cctcggggga ggatgtccac
      301 cgcaagacgt ccgggaactt gatcagctcc tgagccaggc gggccatgtc ccgcggcgtg
      361 gtgacatccc cttcctggcc gggacccggc ggcagaccgt ggaccgaccc gaagcgcgtg
      421 cgcgtgagct tgagcgccgc cgctcgctcg ttcatccgtg ctatcgtcgc ctcgagactg
      481 ccggtcaatc gctccgccaa cgccgtagcc gcgtcattgg cggagccgat gagcaacgcc
      541 gccatcatgt cctcgacggg gaaagtctcc ccctccttca gatacacctg ggagcccccc
      601 attttgctcg ccaggcgaga ggtctgcacc gtatccgaga gcttgagctg gccgtccctc
      661 accgcctcca tcaccaggag ggccagcatc accttcgtca tggaggccgg cgcacccggc
      721 cgatccgggt cctgactcgt caggacgtct ccggtggcgg cgttgatcac gatccatgac
      781 ggcgtggcag gccccgccac cctggctggc ttcggaggat gctgtcctcc cttcggggcc
      841 gcacggcgtc gcgccccgac cgccggggcc tccagcagaa gcacggcgca aaggacgatg
      901 agaatcagac cgcgccagcc cccccggagc cctccgcaga gatccttcag catcccgcag
      961 gtcacatttc tcgtatcgag catgcacacc ctccctcgtt cgcgagcggt cagaattgga
     1021 acgtcaccgt ggtccgagcc gtggccgacg gggcaggagc atgccacgca cccttggaaa
     1081 cgtcaaccat cattcgctca ttgcgaaaca atcgggggct ttccccaagt tcgccaatga
     1141 taacagccag tggccgtcca ccgggtcctg ttgccagatt cgcgtccgtg gtgggcccgc
     1201 ggaggtgttg gcgccaccag cggtgagcag ataagagctt gtcctcccgc acggaaatca
     1261 gtacactccg gccaatattt gggagggcgc gtagccgatg ctctcgctgg gcattgatta
     1321 tgaagccggc caatggaagc ttgccgcctg gaatgaggac cgggctgccg atctgctccg
     1381 cttcggctcc gaaggcgacg tgtgggaatc cgtggaggat atcctcagcg cccatccggc
     1441 cacacccatc gtgcttccct ccgggttcgg catcccggtg acgcgcgcac gggaacttct
     1501 cgaccgggac atcgccgaga taacgctgca ccgcaaggcc cacgtcaccg atcccctcgg
     1561 ccagttcctc gccgaggccc ggcgccgacc cctgcgtgct ttctgtatcc cctccgtccg
     1621 gttgctcccc tcgatccccc ttcatcggaa gctcaatcag gtggacttgg gcaccgcgga
     1681 cgtgttgtgc gcggctgcct gggccatcca ctgcctggcg cagggggggc cgacctccgc
     1741 aacttcacac ttcctcttgc ttcacgttgg ggctgacacg agggccctgg tcgccatccg
     1801 ggacggccgc atcgtcgacg ggatcggcag aagtgcttgg ggaatgggga ccgcgtcgcg
     1861 ggattccgca gacgggttcc tcacctccct cggacagggg attgggcgga gccacgccgc
     1921 cggtggcaca gagaaactcg aaaaccgcct gcgtgaggcc cggaaccacg cgttctggga
     1981 aacagtggag aaagaatccg tttccctcct tgccttccac ggcttgtccc aagtcgtcgt
     2041 caccggtccc gggagcgccc aggccctcca ggcggccgga gatcgccttc ccatcacccc
     2101 tctccccagc caagtggatg gctacgaggc ggccctggga gccgcgctca tcgccgccgg
     2161 cctgaccggc ggtcccaccg cgaacctggt ggatcacctc gaactccgcc aggtgcggga
     2221 gcgggtcttc gactggattg ttccctaaga ccacctcctc gcttcacgcg cgcaatcgcc
     2281 cccgtggaac cttgtgccgg cacgcgccgc catctcagtc gcttccctcg gatccggcgc
     2341 caaagcacac gcatcccggg aaaatacctg cgccgttatc atgagacggc gacatcaccc
     2401 gtgcgcagcg gggcggtcgg aatctgctgc ggctgtcgca agccaacggg ccgtgcggag
     2461 cagaggagca tcccccagtt tcttccccac cacctgcgct cccatcggta accccgcggc
     2521 accgcgcagg atcgggagcg tcaccgccgg cagaccgcag agcgtccaga tcgtgcagaa
     2581 aatagggctg ccggtgcttt cgagacccag aggtgcctgg ctggtcgccg ctggggtgag
     2641 gatggcatcg aagcgggcga agatggggtc gaggtcccga gccaggttcg ggatcgccgc
     2701 cacggcgcgg ttgtattcgg tcgccagcgt ccgttggcct cgctcgatca tctctcgcag
     2761 cctggcgctg agctgccctg ccccccgctc gtactccgcg gcgaaacttc gcgccaaatc
     2821 ggcttccagg atcgtccgat ggacctcggc cgctttggcg aaggccggcg gcaggtcgac
     2881 ctcattcacc tgggtgccga ggcgggccgt gagtcgcgca aactccgcct gcgtgtcctc
     2941 ggccgcctca tcccagaagg gcgacttgac gaaagccagg caaggttgca cggggggcgc
     3001 ctggggcatg acatcggcca gacggggccg cgcctgcggc cgcatggcgg catcccgcga
     3061 atcgaaggcc atcagctccg cggcgagata cgccacatcc gccagcgtcc gagcgaagac
     3121 ccccacgtgg tcgagatgcc aggattgggc caggacgccg tgccgggaga cgagtccgta
     3181 gctcggtttg aatccataga ccccacaata ggcggcagga cggatgaccg acccgttggt
     3241 ctgactcccg acggccaggg ggatcattcc ggccgccacg gcggccgccg agccgctgga
     3301 tgagcccccc ggtgtgtagc ggcggtcgtg ggggttcgtg gtctttcccg ggaagtagac
     3361 ggccagctcc gtcgtcaccg tcttccccag gacgactgcg cccgcggcgc gcaacaggga
     3421 gaccaccgtc gcatcctccg agggacggcg cccggcgtga agaacggtgc cgttctcggt
     3481 cggcatgtcc gccgtgtcga agatgtcctt tacccccacc gggactccgt gcagcggacc
     3541 cagcggcacc ccctgggctt gcgcccgatc ggcggcgcgc gcctgggcca gggcatactc
     3601 ggcgtccagg aaggcccagg cctgcaccac gggttcgacc tccgcgatac gttccagaca
     3661 agcccggacg agctgctccg agctgatatc gccacggcgg atggcctgag ctgcggccac
     3721 agcctccagg gcggcaagcg tgcttcgcat acgatgtctc cagctcacct catgcgggag
     3781 agagcggaca ggcggcctgg gcggaaggaa tggacaggca ccgggactgg gctggggtga
     3841 tggtgggcgc tactgggatc gaaccagtga cctccgccgt gtgaagacgg cgctctaacc
     3901 agctgagcta agcgcccacg cgagatcgcg aggaagcccg tgtctgggac gggctttttc
     3961 ataccacaga gacttatcca ggtccaaggg aattttgtcc cacctgccgg tgtgacccga
     4021 gagagcgatc cctatcccca cacgatgcag ttgaggctgt agggggccgc aagatcctca
     4081 tgcgccggca ggatgcgcac ccggaagcca tgctgtccgg tcgatggggc tggtaatgcc
     4141 cccgagtaga catacacgcc gggcctgata acgccctcga ccttcatggc ctcggcagtg
     4201 gcggtcgtga tctccccctc gcccgtcagg ggacccgcgt acagggcgac cgacacgtcc
     4261 tcgggttgga gcgatcccag cgccacttcg acccacacct ctcgcgtggc gccggccgtg
     4321 atcggcgtga gcatgtccgc cgtcacggcc tggaccgcca cttcgcgcca gcgggcgcgc
     4381 acccgctcct tccaggccg
//
LOCUS       DBNGZC010000279         2023 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1196598, whole genome shotgun sequence.
ACCESSION   DBNGZC010000279 DBNGZC010000000
VERSION     DBNGZC010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1196598"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /gene="tnpC"
                     /locus_tag="AC16G0_05140"
     CDS             <1..843
                     /gene="tnpC"
                     /locus_tag="AC16G0_05140"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HIR5099042.1"
                     /translation="MVQRVLWSTIIHTDDTPVPVWDPTLPHTRTGRFWVCIGDVRHPY
                     VVYDYTPRRTRDGPEQFLKGFRGYLQADAFSGYDRMCAGPDVIEVACWAHVRRKFFES
                     RTSAPVLAHAALARIRQLYAVEHAAEDLSAEGRCALRQRDAVPLLTAFEEWLTEQGHR
                     ALPKSPIGQAIAYAQSNWTALCRYPEHGDLSIDNNLAERMLRAQAIGRRNWTFLGSDR
                     GGRTAAVLYSLTGSCRHHDIDPFAYLQDILSRLPSLPPGQLPELLPDRWFTSHPSARR
                     KTAA"
     gene            1009..1692
                     /locus_tag="AC16G0_05145"
     CDS             1009..1692
                     /locus_tag="AC16G0_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099043.1"
                     /translation="MNTPRPGKTIAQLFEEFLADQQARLSPKTYAKYEGIIHLYQSYL
                     ESYWPGHSEKEYDAITKAKGTYCGTFGAEDIASGFSEFLGYFMPRKVIAGNETMKAAG
                     TVIKKLAKWLVEKGHTEDDESIRELVGETARDLPASQKLLDRLDDWLAENAPEQYGRE
                     VEGHFGIKRVEPGRLWLEPILSGERAIGPIPVPEKVTRACKVGWDVGGVVGETAKGWR
                     LVEVWNISP"
     gene            1689..>2023
                     /locus_tag="AC16G0_05150"
     CDS             1689..>2023
                     /locus_tag="AC16G0_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099044.1"
                     /translation="MRVAEDGKRDARHMAKKKSAARADQPRPSKVLRALREALGLDGE
                     DWQAYLWHARHVFADYAQANWMAPTSTLRLRDVPAEWFEDPEALGYVAVRVLTEGEAD
                     LVTPVARHF"
BASE COUNT          351 a          665 c          685 g          322 t
ORIGIN      
        1 atggtccagc gggttttgtg gtcgacgatc attcacacgg atgatacccc cgtgccggtg
       61 tgggacccga cgctgccaca cacgcggacg gggcggtttt gggtctgtat cggcgatgtc
      121 cgccatccct acgtcgtgta cgactacacg ccgcgccgga cgcgggacgg gccggagcag
      181 ttcctgaagg ggttccgggg ttacctccag gccgacgcgt tctccggcta cgaccggatg
      241 tgcgccggtc cggacgtgat cgaggtcgcg tgctgggccc acgtgcggcg caagttcttc
      301 gagtcgcgga cctccgcgcc ggtgctggcg cacgcggcgt tggcccgcat ccggcagctc
      361 tatgcggttg agcatgccgc tgaggatctc tcggccgagg gccgttgcgc cctgcgtcag
      421 agggatgcgg tcccgctgct gacggccttc gaggagtggt tgaccgaaca gggtcaccgc
      481 gcgctgccca agagcccgat cggtcaggcg attgcctacg cgcaatccaa ctggacggcg
      541 ctctgtcgct atcctgaaca tggggacttg agcatcgaca ataacctcgc cgagaggatg
      601 ctgcgtgccc aagcgatcgg acgccggaat tggacctttt tggggagtga ccgtggcggc
      661 cggacggcgg cggtcctgta ctccttgact ggcagctgca ggcaccatga catcgacccg
      721 ttcgcatacc tccaggacat cctgagtcga ctgccatccc tgccccccgg acagctcccc
      781 gagctgttgc ccgaccgctg gttcacctca cacccctcag cacggcgcaa gacagccgcg
      841 tgatccgact gctccgaagg cgacaattcc tgacgtgggc gtcgatccgc tcccaacagg
      901 gtcgtctccg gcgcgatcgg ccggatcatc accaaggggg cgtggtcggc gcgaccggct
      961 cggatagaat gcgagacggc gcacgccatc ctgagacctg agggcccgat gaacacaccc
     1021 aggccgggca agaccatcgc gcagctcttc gaggagttcc tggccgacca gcaggcccgg
     1081 ctcagcccga agacctatgc caagtacgaa gggatcatcc atctctacca gtcgtacctg
     1141 gagagctact ggcccggtca ctcggagaaa gaatacgacg cgatcaccaa ggccaagggc
     1201 acctattgcg gcaccttcgg ggccgaggac atcgcctccg ggttctccga gttcctcggc
     1261 tatttcatgc cccgcaaggt catcgccggc aacgagacga tgaaggctgc cgggacggtc
     1321 atcaagaagc tcgccaagtg gctggtcgag aagggccaca ccgaggacga tgaatcgatc
     1381 cgggagctgg ttggcgagac cgcgcgcgac ctgcccgcca gccagaagct cctcgatcgg
     1441 ttggacgact ggctcgccga gaacgccccg gagcaatacg ggagggaggt cgaggggcac
     1501 ttcgggatca agcgcgtcga gccgggccgg ctctggctgg agccgatcct ctccggcgag
     1561 cgcgcgatcg gcccgatccc ggtccccgag aaggtcacgc gggcctgcaa ggtgggctgg
     1621 gacgtcggcg gcgtggtggg ggagacggcc aaaggctggc ggttggtcga ggtctggaac
     1681 atctcgccgt gagggtcgca gaggacggga agagggacgc gcgccacatg gccaagaaga
     1741 aatcggcggc acgggccgat cagcctcgac catccaaggt ccttcgcgcg ctgcgtgagg
     1801 ccctcggcct ggacggggag gactggcagg cgtacctctg gcacgcgcgc cacgtcttcg
     1861 ccgattacgc gcaggccaac tggatggccc caacgtccac gctccggctc cgcgacgtgc
     1921 ccgccgagtg gtttgaagat cccgaggccc tgggctacgt cgccgtacgc gtcctcaccg
     1981 agggcgaggc ggacctggtc actccggtcg cccgccactt ctt
//
LOCUS       DBNGZC010000280         1557 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1294533, whole genome shotgun sequence.
ACCESSION   DBNGZC010000280 DBNGZC010000000
VERSION     DBNGZC010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1294533"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="AC16G0_05155"
     CDS             complement(<1..414)
                     /locus_tag="AC16G0_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR5099045.1"
                     /translation="MPEYEAVIGLEVHAELLTESKMFCGCSTRFGAPPNSQTCPICLG
                     MPGVLPVINRRGVEFAIRTALALNCSIAPMSRFARKNYFYPDLPKAYQISQYELPLSS
                     HGHIEFFLNGTKKRVGITRLHMEEDTGKLVHAGIME"
     gene            complement(417..860)
                     /locus_tag="AC16G0_05160"
     CDS             complement(417..860)
                     /locus_tag="AC16G0_05160"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF017868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HIR5099046.1"
                     /translation="MLYFAYGSNMHPGQMWKRCPGCAFVATAQLKNHRLTFSRFWGAW
                     GGGGVADIQPAPGSVVEGVVWEITGSHRQSLDDYEDYPTSYSRKEVSVETPEGRMLTA
                     FAYVARPTGVYRPARRYLVEILEGARAHKLSQGYIAVLEAIPTED"
     gene            complement(963..1325)
                     /locus_tag="AC16G0_05165"
     CDS             complement(963..1325)
                     /locus_tag="AC16G0_05165"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HIR5099047.1"
                     /translation="MARRRGVGARVALGICLVLVVLAVVPAWAASGGKNRDLAVLDHE
                     RWLDTLKGTVKNFSKANARDVTVVVRFLDGKKKALGTQRVTVGDLNSGEQSSWSLPIE
                     ERNRPAKTYKFEVHAIWR"
     gene            complement(1343..>1557)
                     /locus_tag="AC16G0_05170"
     CDS             complement(1343..>1557)
                     /locus_tag="AC16G0_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HIR5099048.1"
                     /translation="DPLQMYLSDIFTISVNLAGVPGISINCGFTQAGLPIGLQIIGKP
                     FDEGTILKVAHAYEQATAWHRERPRL"
BASE COUNT          264 a          517 c          476 g          300 t
ORIGIN      
        1 ctccatgatt cctgcatgca ccagcttccc cgtatcctct tccatgtgga ggcgcgtgat
       61 gccgactcgc ttcttggtcc cattcaggaa gaactcgatg tggccgtggc tggacagggg
      121 cagctcgtac tgggagatct ggtacgcctt cggcaggtcc gggtagaagt aattcttgcg
      181 ggcgaagcgg gacatgggtg cgatgctgca gttgagcgcc agggcggtgc gtatggcgaa
      241 ctccaccccg cggcggttga tgacggggag gacgccaggc atccccagac agatggggca
      301 ggtctggctg ttgggtggcg ccccgaatct cgtgctgcac ccgcagaaca tcttggattc
      361 ggtcaggagt tccgcgtgca cttccagacc gataacggct tcgtattcag gcatcctcaa
      421 tcctcggtag gaatcgcttc cagcacggcg atgtagccct ggctgagttt gtgggcgcgc
      481 gcgccttcaa ggatctctac caggtatcgg cgggccggtc ggtagacccc cgtcggcctg
      541 gccacgtacg cgaacgctgt cagcatcctc ccctcaggag tctcgacgga aacctccttc
      601 cgcgagtagg acgtcgggta gtcctcgtaa tcgtccaggg attggcggtg tgacccggtg
      661 atctcccaaa cgaccccctc cactacgctg cccggcgccg gctggatatc cgccacgccg
      721 cccccgcccc aggcgcccca gaaccggctg aaggtcagcc gatgattctt gagctgcgcc
      781 gtggccacga atgcacagcc ggggcatcgc ttccacatct ggcccggatg catgtttgat
      841 ccgtaggcaa aataaagcat ggggcaagct caaaagcgtt cggctgctcg gccgctcggc
      901 tattcgggtg ttccggaact gccgaacggc cgaaccctcg aacagccgaa cgtcttgcgg
      961 tcctatcgcc agatggcgtg gacctcgaac ttatacgtct tcgccggacg gttccgctcc
     1021 tcgatgggca ggctccagga actctgctcc ccagagttga gatcgcccac ggtcacccgc
     1081 tgggtgccca gggccttctt cttcccgtcc aggaacctca cgaccacggt aacgtcgcgg
     1141 gcgttcgcct tgctgaaatt cttgaccgtc cccttgagcg tgtccagcca tcgctcatgg
     1201 tccaggaccg ccagatcccg gttctttcca ccagacgcgg cccaggccgg caccacggcc
     1261 aggacgacca ggaccaggca gatcccaagc gcgacgcgtg ctccgacacc tctccggcgt
     1321 gccatggcct cccgccttcc tcctacagcc gcggccgttc cctgtgccag gccgtggcct
     1381 gctcgtaggc gtgtgcgacc ttcaggatcg ttccctcatc gaacggcttc ccgatgatct
     1441 gcaggccgat gggcaggccg gcctgcgtga agccgcagtt gatgctgatc cccggcaccc
     1501 cggcgaggtt caccgagatg gtgaagatgt ccgagaggta catctgaagc gggtcgg
//
LOCUS       DBNGZC010000281         7239 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1097788, whole genome shotgun sequence.
ACCESSION   DBNGZC010000281 DBNGZC010000000
VERSION     DBNGZC010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7239)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1097788"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..1782)
                     /locus_tag="AC16G0_05175"
     CDS             complement(274..1782)
                     /locus_tag="AC16G0_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099049.1"
                     /translation="MLILPFMLGANVAHAAKDKVVVAFGSNIPTLDPHMHSSRLAHIA
                     DWHLYDTLMDRDPKTYKPRPGLASSLKNLNPTTWEVKLRTGIKFHNGEPFNAEAVKFT
                     LDRVLDPATKSVTRGNFTWIKEVKILDDTTVHIITAKPFPAAAEFLTLAYIVPPKYLK
                     AVGDEEFSRKPVGTGPYKFVEWKKAEHLIVEANENYWKASPKGMPKIKTIVFRTIPET
                     TTQIAELLSGGVDIIRNVPPDQIDVIKNSPNARISATKILRVNSLLLDSAGRASKTPL
                     MNQKVREAIAYAINVDEMLKQILGGYAERTATGVNPLHFGFDPSIKPYPYDPDRAKKL
                     LTEAGYPNGFEITLNTYSGTITSMDQMADAVTGYLAKVGIKVRRRHIEDVGMWTSSGK
                     EGKLEGIQYSSWGSNSIFDADAILYALTYSKEPLSYTKDDTLDQFLDEGRTQIDPKKR
                     LEAYAKAQKLLHEKFYWIPINVQYTIEGINKQLNYEAASDEMMRVYSASWKD"
     gene            complement(1871..3217)
                     /locus_tag="AC16G0_05180"
     CDS             complement(1871..3217)
                     /locus_tag="AC16G0_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017248438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5099050.1"
                     /translation="MQEIFRYVDQNRERFLGELFALLRQPSISVANQGVTECAELLKG
                     QMEDIGIPARILSTAGHPVVYGEVKAAGAKRTVLIYGHYDVQPPDPLESWKSPPFEPT
                     IRDGRIYGRGTGDNKGQFFAHLKAVEAILKVRQTLPVNVKFLFEGEEEISSRSLRAFM
                     QEHRELLSADYAYCSDGGMQPGDQPTIVFGVRGILYVEVTAAGANRDVHSGNLGAFVP
                     KPAWRLVHFLNTLVDETGRVRVNGFYDAVLPPNDVEREALGKIPFDPQVLRDLGVSGE
                     TKPGEPPFSERVMFHPSMNICGLVSGYGGPGMKTIVPHTAKVKLDMRLVVNQDPDDIF
                     QKFTAHARQHGFGDLRIEKLGAFYPSRTPITHPLGKAAAAAVRIGLGKEPLLIPCMGG
                     SDPDYYFTKILGVPRVNVPYAPHDENNHAPNENIRLEGFFSGIKTTAAFLHEAAQL"
     gene            3687..4697
                     /locus_tag="AC16G0_05185"
     CDS             3687..4697
                     /locus_tag="AC16G0_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012247436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099051.1"
                     /translation="MKLLGRLLVIAATFTLSCSPAASAETGELRVAKQYGLGYLQMML
                     MEEQKLVEKHAKAAGLGDLKVTWATFRSSDVMNDALLSGNLDFACLGTNGLATIWAKT
                     RGTAVEVKAASGFNFFPLALVTRDSNVKSLSDYTDKHRIAVPAIKVSNQALFLQMAAA
                     KLYGQANYTKFDPLTVTMSHPDAMAALLSGAGEISSHFASPPFLQKELEKPGLHKVIS
                     GPEIVGGPYSFNLIGAPTKFYTTNPKTFVVFLAALQEATDIINQNKRAAAEVYIRMTK
                     DKSSTDEILKIMNDAGNEYSLLPKGNMPVVEFMHKIGVIKVRPESWKDLFFPNVHGQP
                     GS"
     gene            4705..5496
                     /locus_tag="AC16G0_05190"
     CDS             4705..5496
                     /locus_tag="AC16G0_05190"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099052.1"
                     /translation="MAPTETTPSLLDVRGVTLQYKTRDHLVTATYRVDFQVRTSDRFV
                     VLGPSGCGKSTLLKAVGGYLPPVEGEIRLKGAPIRKPGPDRMMVFQEFDQLLPWKTVK
                     QNVSFALTASGLLPGRAAEERAMHYIRKVGLAEFADAYPHTLSGGMKQRVAIARGMAM
                     EPAILLMDEPFAALDALTRRKMQEELLRLWDDTQFTMLFVTHSIPEAITVGSRILLLT
                     PRPGQVKAELNSVPRDDTDLERHTALEDRIHRMLFADQVEEGMPR"
     gene            5556..6431
                     /locus_tag="AC16G0_05195"
     CDS             5556..6431
                     /locus_tag="AC16G0_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012321293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099053.1"
                     /translation="MTEFAVRPDRPDVIRETVSPEAFGILQQPLTAWERAWNNGALRK
                     AVLLIVLASAWEVYARMLNNPLLVPTFSATLEALWEGVTAGPLLGRALFSIRVLLMGY
                     AAGIGIAAVLTALAVSSQVGTDLLETLTSMFNPLPAIALLPLALLWFGLGNPSLIFVM
                     VHSVLWAVALNTHSGFTSVSNTLRMVGQNCGLSGPRYVLRILIPAAFPSILTGLKIGW
                     AFAWRTLIAAELVFGVSSGSGGLGWFIYENKNLLEVPSVFAGLFSVILIGLIVENLVF
                     RNIEVRTVRRWGMQG"
BASE COUNT         1291 a         2237 c         2321 g         1390 t
ORIGIN      
        1 agccccaagc tctgtcgcag gatcgcggcg tcttcggctt tcgcctcgat ggacaagagg
       61 agctggacgg gatcccccgc aacgtgcagc agcaggaagg tgatcaccga gatccctaca
      121 catactagca ctgcctcgcc caggcgcttc aggatgtata tggacatgac tcaccagacg
      181 ggcgctgcag cgtggaggcc cggaagcgaa aagtcgcagc ggccattccg cctcgacgcc
      241 tccgcgcccc tgggcctcgg cgtcagttcc ctctcagtcc ttccaggaag ccgagtacac
      301 ccgcatcatc tcgtcgctcg ccgcttcata gttgagctgc ttgttgatgc cttcgatggt
      361 gtactggacg ttgatgggga tccagtaaaa cttctcgtgg aggagcttct gcgccttcgc
      421 gtatgcctcc aggcgcttct tcggatcgat ctgggtccgg ccctcgtcga ggaattgatc
      481 cagggtgtcg tccttcgtgt agctcagcgg ctccttggag tacgtcaggg cgtagaggat
      541 ggcatcggcg tcgaagatgg agttggagcc ccagctagag tactggatcc cctccagctt
      601 gccctccttg cccgaggacg tccacatgcc cacgtcttcg atgtggcggc gcctgacctt
      661 gatgcccacc ttggccagat agccggtgac cgcatcggcc atctggtcca tgctggtgat
      721 ggtgccgctg taggtgttca gcgtgatctc gaacccgttg ggatacccgg cctcggtgag
      781 gagcttcttg gcccggtcgg ggtcataggg atagggcttg atggacgggt caaagccgaa
      841 gtgcaaaggg ttcacgcccg tggcggtccg ctcggcgtag ccccccagga tctgcttgag
      901 catctcgtcc acgttgatgg cgtaggcaat ggcctcccgc accttctggt tcatgagggg
      961 ggtcttgctg gcgcgtcccg cgctatccag gaggagggaa ttcacccgca ggatcttggt
     1021 cgccgagatc cgggcgttgg gtgagttctt gatcacgtcg atctggtcgg gcgggacgtt
     1081 ccggatgatg tccacgccgc cggacagaag ctccgcgatc tgggtcgttg tctctgggat
     1141 cgtgcggaat acgatggtct tgatcttggg catgcccttg gggctggcct tccagtagtt
     1201 ttcgttggcc tccacgatca ggtgctccgc cttcttccac tcgacgaact tgtaaggtcc
     1261 cgtgcccacc ggcttgcgcg agaactcctc gtcgccgacg gccttcagat attttggcgg
     1321 cacgatgtag gcgagcgtca ggaactcggc ggcggccggg aacggtttgg cggtgatgat
     1381 atgcaccgtg gtgtcgtcga ggatcttgac ttccttgatc caggtgaagt ttccgcgggt
     1441 cacggacttg gtggccgggt cgaggacgcg gtcgagggtg aacttgacgg cctcggcgtt
     1501 gaacggctcg ccgttgtgga acttgatgcc ggtccggagc ttcacctccc aggtcgtggg
     1561 attgaggttc ttcaacgagg aggcgaggcc ggggcgcggc ttgtaagtct tggggtcccg
     1621 gtccatcaag gtgtcgtaca ggtgccagtc ggcgatgtgg gcgagtcgac tgctgtgcat
     1681 gtggggatcc agggtgggga tgttggatcc gaaggctacc accaccttgt ccttggcggc
     1741 atgcgccacg ttcgcgccca gcatgaaagg gaggatgagc atgggaacga agagcctgat
     1801 cctgaatctg cagcgtccgt ccatggagcc ctccttcttc ggggatcgac ctcagacaac
     1861 tgcctcgggt tcagagctgt gccgcttcgt gcaagaaagc ggcggtggtc tttatcccgc
     1921 tgaagaaacc ctccagcctg atgttctcat tgggagcatg gttgttctca tcgtggggcg
     1981 cgtagggcac attcacccga ggcaccccca agatcttcgt gaagtagtaa tccgggtcgc
     2041 tgccgcccat gcagggtatg agcagcggct ctttgccgag cccgatccgg accgccgctg
     2101 cagccgcttt ccccagagga tgggtgatcg gcgtgcggga gggatagaag gcgcccagct
     2161 tctcgatcct gaggtcgcca aacccgtgct gccgcgcgtg ggccgtgaac ttctggaaga
     2221 tgtcatccgg gtcctggttc accaccagcc gcatgtcgag cttcaccttg gcggtgtgag
     2281 gaacgatggt cttcatgccc ggtccgccgt atccactgac aaggccgcag atgttcatgc
     2341 tggggtggaa catcactcgc tcggagaagg ggggctcgcc gggtttcgtc tcgcccgaga
     2401 cccccagatc cctgaggacc tgtggatcga agggaatctt gccaagcgcc tcacgctcca
     2461 cgtcgttggg cggcagcacc gcatcgtaga aaccattcac gcgaacccgt cccgtctcgt
     2521 ccaccaaggt gttcaggaag tggacgagcc gccaagccgg cttgggcacg aaggcgccca
     2581 gatttcccga atgcacgtca cggttggcac cagcggcagt cacctcgaca tataggatgc
     2641 cccggacacc aaacacaatg gtgggctggt cgccgggctg catgccgccg tcggagcaat
     2701 aggcgtagtc cgccgacagc agctctcggt gttcctgcat gaaggccctg agactgcgac
     2761 tgctgatctc ctcctcgccc tcgaagagga atttcacatt gaccggcagt gtctgcctga
     2821 ccttcaggat cgcctccacg gccttgagat gggcaaagaa ttggcccttg ttgtcgcccg
     2881 ttccgcggcc gtagatgcgg ccgtcgcgga tcgtcggttc aaagggcggg gacttccatg
     2941 attcgagcgg atcggggggt tggacgtcat agtgcccgta gatcaggacc gtgcgcttgg
     3001 ccccggcggc cttcacttcc ccatagacga cgggatgtcc ggccgtggag aggattcgag
     3061 ccgggatgcc gatgtcctcc atctggccct tgagcagttc tgcgcactcg gtcacgccct
     3121 ggttggctac gctgatgctg ggctgccgga gcaaggcgaa cagctcgccc aggaaccgct
     3181 cgcggttctg gtccacgtac cgaaatatct cctgcatagg gtcccccaga gaggtgtgga
     3241 gtgccgtgga tagaaaacgc ccgaagagga aaaaggaggc tgcctcccag gcaggggttg
     3301 tagcgttgcc tgcccacctt tgtcaagcaa acgcattcga ttgcgcggtt gatctcccaa
     3361 atcggcgccc gacgaatcag gatggggagg tgcctcctct ggccggcggg aagatacagg
     3421 tttctggaca accggcgatt ccattcgaat ttccgttgct tgccggtcgc ggtcagatta
     3481 gggtcggcga ccgggaggcc tcgggctcgc aggcacgccg gaagaaccag ccaatcaggc
     3541 gtgggctcag aacgtggctg ggatgcggcc tgcggcatgc tccgaccggg gtctcgtcac
     3601 gaggtcgcca ggggcctggg tggctccaag gcattggact cgggacggcc gattcagaag
     3661 cgcaacagga cagaggagga atcagcatga agcttctggg cagactgctt gtgatcgcgg
     3721 cgacattcac gctgagctgc tcgcccgccg catcggcgga gaccggtgag ctgcgcgttg
     3781 ccaagcaata cggactgggc tacctgcaga tgatgctgat ggaggagcag aagctggtgg
     3841 agaagcatgc caaggcggcg ggcctcggcg acctcaaggt gacctgggcg acgttccgct
     3901 ccagcgacgt catgaacgac gcgctgctct cggggaacct ggacttcgcc tgtctcggca
     3961 ccaacgggct cgccaccatc tgggccaaga cccgcggcac cgcggtggag gtcaaggccg
     4021 cctccggatt caacttcttc ccgctggcac tcgtgacccg ggactccaat gtcaaatccc
     4081 tctccgacta caccgacaag caccggatcg cggtgcccgc catcaaggtg tcgaatcagg
     4141 ccctcttcct gcagatggcc gccgccaagc tctacgggca ggcgaactac accaagttcg
     4201 accccctcac cgtgaccatg agccaccccg acgccatggc ggcactgctc tcgggggcgg
     4261 gcgagatcag cagccacttc gcctccccgc cgttcctgca gaaggagctg gagaagccgg
     4321 ggctccacaa ggtcatcagc ggacccgaga tcgtcggcgg gccctactcg ttcaacctca
     4381 tcggggcacc caccaagttc tacacgacga atcccaagac tttcgtcgtc ttcctggcgg
     4441 ccctgcaaga ggcgacggac atcatcaacc agaacaagcg ggcggcggcg gaagtctaca
     4501 tccgcatgac caaggacaag agctcgaccg acgagatcct gaagatcatg aatgacgcgg
     4561 gcaacgagta cagcttgctc cccaagggga acatgccggt ggtcgagttc atgcacaaga
     4621 tcggcgtgat caaggtcagg cccgaatcgt ggaaagatct cttcttcccc aacgtgcacg
     4681 gccagcccgg gagctgatcg ccgcatggcg cccacggaaa cgacgccctc actcctcgac
     4741 gtccggggcg tgacgctcca gtacaagacc cgggatcatc tggtcaccgc cacataccgg
     4801 gtggatttcc aggtccgcac ctcggaccgg tttgtcgtgc tcggtccctc gggctgcggg
     4861 aaatcgaccc tgctgaaggc ggtgggtggc tacctgcctc cggtggaggg cgagatccgc
     4921 ctcaagggcg cacccattcg gaagcccggc ccggaccgga tgatggtgtt ccaggagttc
     4981 gatcagcttc tcccgtggaa aaccgtgaag caaaacgtca gcttcgcgct cacggcgagc
     5041 ggcctgcttc cgggcagggc ggctgaagag cgggcgatgc actacatccg gaaggtgggt
     5101 ctggccgagt tcgccgacgc ctatccacac accctgtccg gcggcatgaa gcagcgggtg
     5161 gcaatcgccc ggggcatggc catggagccg gccattctgc tcatggacga acccttcgcc
     5221 gccttggatg cgctgacgcg ccgcaagatg caggaggagc tgctgcgtct gtgggacgat
     5281 acgcagttca ccatgctctt cgtcacccat tcgatccccg aggcgatcac ggtcggcagc
     5341 cggatcctgc tgctcacacc ccgtccgggt caggtcaagg cggagctgaa cagcgtgccg
     5401 cgcgatgaca ccgacttgga gcggcacacg gccctggagg accggatcca ccgcatgctg
     5461 tttgccgatc aggtcgaaga gggtatgccg cgttgatccg gatcgccgcc ggcctgcctg
     5521 tggccggcgg ccccggagga ctcgagggaa gcgccatgac agagttcgcc gtcagaccgg
     5581 atcgtcccga cgtcatacgc gagacggtgt caccggaggc cttcgggatt ctccagcagc
     5641 cgctgaccgc gtgggaacgc gcctggaaca acggcgcctt gcggaaggct gtcctcttga
     5701 tcgtgctggc gtcggcctgg gaggtctacg cccggatgct caacaatccg ctgctggtgc
     5761 cgaccttcag cgccaccctc gaggccttgt gggagggtgt caccgctggc ccgctcttgg
     5821 gtcgggcgct gttctcgatc cgggtgctct tgatgggcta cgccgcgggc atcgggatcg
     5881 ctgcggtgct caccgccctg gccgtcagct cgcaggtcgg caccgacctc ctggagaccc
     5941 tgacctcgat gttcaatccg ctgcccgcca tcgccctgct gcccctggcc ctgctgtggt
     6001 ttggcctggg caaccccagc ctcatattcg tcatggtgca ttcggtcttg tgggccgtgg
     6061 cgctgaacac ccattcgggg ttcacctcgg tctccaacac cctgcgcatg gtggggcaga
     6121 actgcggcct ctccgggccc cgctacgtgc tgcggatctt gatcccggcg gccttcccca
     6181 gcatcctgac cggattgaag atcggatggg ccttcgcctg gcgcaccctg atcgcggccg
     6241 agctggtctt cggcgtgagt tccggttcgg gcggcctggg gtggttcatc tacgagaaca
     6301 agaacctgct ggaggtcccc agcgtgttcg ccggactgtt ctccgtcatc ctgatcgggc
     6361 tgatcgtgga aaacctggtg ttccgcaaca tcgaggttcg caccgtgcgt cgctggggga
     6421 tgcagggcta gagccggtcc gtggttcttc agcgcaggaa cgccacgaac ggaagaattg
     6481 acccgcctgg aggatatcga attctaaagg cagaggggac gcatgtcccg atcggtcatc
     6541 gtatcccgtg cgattccaag agagcatatg ggagtaagga gagacgcagg gaccgaaggg
     6601 gaaggaggga tcagcgatcc gtattggatg tctcgggaag ggctcgggat cgcctctccg
     6661 atgagtaccg ctctggaagc ccggacgcgg tgcgcaagcg cgaacgtcca ggtgaacttc
     6721 taaccgagag attcgccaaa ctccctgggg ggaatcttgt cagggccttc gggtggtccc
     6781 ggggtaccct gcttcaaccc acacatacgc tcaaggcatt ccccgcagat tcggaatccg
     6841 ccttcgtccc gtgaccactc gtccccgcct tcgcgcggcg cttcgccatg tcgaccgcag
     6901 tgctcgcaga tgaagacgag gaactcagtt gccagaggat gtctccccgg ctggcgcctc
     6961 gacgatcggt ggatgatcgc ggaagcatcc gcaagcagtt tccagaaaac tccccagagt
     7021 ccacgggcgc tcacctgctt acaactcctc ttgtcaggag ggggcaccga cgccaagatc
     7081 agagatcaaa tggctgtccg ctcagaagta ttgatgaccg ccttgagcag gtcggggagc
     7141 gccagcgaat tctgattatc caccgtcgcg aacttccccg atgcctttcc ctggtcggca
     7201 aaaccggcgt ccacgtacaa tccttcgaca gtcttggga
//
LOCUS       DBNGZC010000282         4596 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2370244, whole genome shotgun sequence.
ACCESSION   DBNGZC010000282 DBNGZC010000000
VERSION     DBNGZC010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4596)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4596)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4596
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2370244"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="AC16G0_05200"
     CDS             complement(<1..334)
                     /locus_tag="AC16G0_05200"
                     /EC_number="1.2.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR5099054.1"
                     /translation="MSSRAVLTGRHFMQGNIACAEGALAAGCRFAAGYPITPATEVAE
                     RLAERLPAIGGTFLQMEDEIGSIAAVIGAAWGGQKAMTATSGPGISLMLENIGFALGT
                     ETPCVIVNV"
     gene            complement(402..662)
                     /locus_tag="AC16G0_05205"
     CDS             complement(402..662)
                     /locus_tag="AC16G0_05205"
                     /inference="COORDINATES: protein motif:HMM:NF024247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5099055.1"
                     /translation="MLKPVIISINENFCKGADGCGICLALCPKDVFKIAERLTARGIR
                     LPVVEKITACSNCGNCVIYCPDMAVVVRLDEVPEAAAAAQQQ"
     gene            complement(752..1984)
                     /locus_tag="AC16G0_05210"
     CDS             complement(752..1984)
                     /locus_tag="AC16G0_05210"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF052515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor-dependent isomerase,
                     group 22"
                     /protein_id="HIR5099056.1"
                     /translation="MALPEVIRVRQTFDRTRLADPCRAVTDGLRALPLATKLRPGARV
                     AITAGSRGISNLVGMTRAAADAVKAAGGRPFIVPAMGSHGGASGDGQRSLLADLGISE
                     ASMGCPVVSCMEVEEVGRTAAGIPVYLDSNAFHADGIIAINRVKLHTIFRGDVESGLC
                     KMLAVGLGKHKGAQQIHKIGVAPILVESAQVILTKAKVLAGIAVLENSLDETMEVHVV
                     PPERFEETDAALLKRVWAILPRVPFDPLDVLVVDEMGKNISGTGMDTNVIGVGGRVGG
                     KMTMGSPVVSAIVVLGLTPETHGNANGIGLADLTTRRLVDSIDYKATYTNVLTTRLWS
                     AGRLPVILETDREAIEVAVGDTPPDRVRFVRIQDTLHLEELEISQALLPQALQTGLTV
                     VREARPLPFDDTGRLAVW"
     gene            2269..3438
                     /locus_tag="AC16G0_05215"
     CDS             2269..3438
                     /locus_tag="AC16G0_05215"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046349 - amino sugar biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIR5099057.1"
                     /translation="MSARLVVRGRLEGATEPVDIAIEDGRVRAIARTVEGDDRSVVGG
                     RDCLLSEAFLDIQVNGFASVDFNRPTLKPAELRRALEAMRRHGVTRFFPTIITAPLQA
                     MLETLQAIAHARQQDEALLHAIPGIHLEGPFLAPEDGPRGAHPVSFIRDPDLELFRQF
                     QEASKGLIRLVTLAPERPGAIELISKLRKAGIVVAIGHSDASEADIDQAVEAGAQLST
                     HLGNGAHAVLPRHRNYIQKQLATDALMASMIADGHHLPGYFVRNFIRCKGLDRVILLT
                     DALAAAAGPPGTYRLGEVLAEVGPDGYVRLPGTPYLAGSALTMDRAVENLARFAGVSL
                     WEAIGLATRQPRRLFPDLGAGIAPGEPADLLVLREGPPLTVVATIAQGELVYRRA"
     gene            3500..4252
                     /locus_tag="AC16G0_05220"
     CDS             3500..4252
                     /locus_tag="AC16G0_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HIR5099058.1"
                     /translation="MDAIAQYHAAVTAMLQQIVDTQREPIARAARSCADAIAEDRYLY
                     VIGTGGHSYIACEEMFWRAGGLVPVYPILDPGFSVSHGAWRSMLIERTPGYVIPILRY
                     HGLGKGDVLIVCNAYGINAATIDAATEGKRLGATVIGVTSTEFATRVPSDHPARHPSR
                     QNLHEIADIFLNTWMPYGDAVVDIPGCPQKVAPASSLGTAFVLHCLVATTVEVLVGRG
                     ITPPVWRSGNLPGGDEANRQHMDKYLRRIKFL"
     gene            4299..>4596
                     /locus_tag="AC16G0_05225"
     CDS             4299..>4596
                     /locus_tag="AC16G0_05225"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR5099059.1"
                     /translation="MRASWKGWMALLAITAMSLAVCETRADAAKVTLKLGHIRADTSP
                     THKAALKFAELVGQKTNGEVEIKVFPNSQLGGILDMFTGMKAGSIDLVYGGINTY"
BASE COUNT          822 a         1504 c         1496 g          774 t
ORIGIN      
        1 tcacgttcac gatcacgcag ggggtctcgg tccccaaggc gaagccgatg ttctccagca
       61 tcaggctgat cccggggccg gacgtggcgg tcatggcttt ctgcccgccc caggcggctc
      121 cgatgactgc ggcgatggag ccgatctcgt cctccatctg caggaaggtg ccgccgatgg
      181 cggggagccg ttcggccaat cgctccgcca cctcggtcgc cggtgtgata ggatagcccg
      241 cggcgaagcg gcagcccgcg gccagggccc cttcggcgca ggcgatgttc ccctgcatga
      301 aatgccgtcc cgtgaggact gcgcgactac tcatagatga ctccattgcg aatgaccgat
      361 gagacgggaa tgaccaatga ccgatggggg aacagaattg gttattgttg ttgagccgcg
      421 gcagcggctt ccggcacctc gtccagccgg accacgacgg ccatgtcggg gcagtagatc
      481 acgcaattcc cgcagttgct gcaggcggtg atcttttcga ccacggggag ccgaatgccc
      541 cgggcggtca gccgctccgc gatcttgaag acgtccttgg ggcacagcgc caggcagatg
      601 ccacagccgt cggccccctt gcagaagttt tcattgatgg agatgatcac tggcttcaac
      661 atgtggcacc cctcgcgcct tcgggcgata tcccaatgac caatgacaac ttaccaatga
      721 ccgagactcg actggtgatg ggtctagtcg attaccagac ggccagacga cccgtgtcat
      781 cgaagggaag cggccgggcc tcccgcacga cggtgagacc cgtctgcagg gcctggggca
      841 ggagggcctg ggagatctcc agctcctcca ggtgaagcgt gtcctggatc cgcacgaatc
      901 ggacacggtc cggcggcgtg tctccgacgg cgacctcgat agcttcccgg tcggtttcca
      961 ggatcacggg cagccggccg gcggaccaga gacgggtcgt gaggacgttg gtgtacgtcg
     1021 ccttgtagtc gatggaatcc acgagacgcc gggtggtgag atctgccagg ccgatgccgt
     1081 tcgcattccc atgggtctcg ggggtgagcc ccaggacgac tatggcggac accacgggcg
     1141 atcccatggt catcttgccg cccacacgcc cccccacacc gatgacgttg gtgtccatgc
     1201 cggtcccgct gatgttcttg cccatctcat ccaccaccaa cacgtccagg gggtcgaaag
     1261 ggacgcgagg caggatcgcc cacacccgct tcaggagcgc ggcatccgtc tcctcgaagc
     1321 gttccggcgg gaccacatgg acttccatgg tttcatccag cgagttctcc aggaccgcga
     1381 ttcccgccaa tacctttgcc ttggtcagga tgacctgagc ggattccacg aggatcgggg
     1441 cgaccccgat cttgtggatc tgctgggcgc ccttgtgctt ccccaggccc accgccagca
     1501 tcttgcacag accgctctcc acatcgccgc ggaaaatcgt gtggagcttc acccggttga
     1561 tggcgatgat gccgtcggcg tggaaggcgt tgctgtccag gtacacaggg atgccggcag
     1621 ccgtgcggcc gacctcctcg acctccatgc aggagaccac cgggcagccc atgctcgctt
     1681 ccgagatacc caggtcggcc aggaggcttc gctgaccatc gcctgacgcc ccgccgtggc
     1741 tgcccatggc cgggacgatg aatggtcggc cacctgcggc cttcaccgcg tctgccgcgg
     1801 cgcgggtcat ccccacgaga ttgctgatgc cgcggctacc tgccgtgatg gccacccggg
     1861 cgccaggccg gagcttggtt gccaggggca aggcacggag accgtccgtc accgcacgac
     1921 aggggtcggc gagccgcgtt cggtcgaacg tctgtctcac gcgaatgact tccggtagtg
     1981 ccatgagtgg ttcctccaga gcgatccaca cgtgcaacgg ggtgcaccat acgccaatcc
     2041 ttcgcaaaac gcatcccgaa gtgtgacctt ggctgcatct tcggacaatc taagcatgtg
     2101 tgcagtccga accggcaggg gcgcaagact tgacaatcat cctgggcgtg tattactaac
     2161 aggcgaaagc gtcggcgcgg agcgtttcgg tccctggtgg ccgtatggac ggcaggaaac
     2221 cgccgccaag tatcccccac tccggaggat acgcggagga gtctctcgat gagcgcacgc
     2281 ctggtggtac gaggccggct ggagggagcg acggagcccg tggacatcgc catcgaggac
     2341 ggtcgcgtcc gagcgatcgc ccggacggtt gagggtgacg accggtcggt cgtggggggg
     2401 cgggattgcc tgctgagtga agcattcctg gatatccagg tgaacggctt cgccagcgtg
     2461 gacttcaacc ggccgacgct gaagcctgcc gagctgcgcc gcgccctgga ggcgatgcgg
     2521 cgccatggcg tcacccgatt cttcccgacc atcatcacgg cgccccttca ggcgatgctg
     2581 gaaacattgc aggcgatcgc ccatgcccgg cagcaagacg aggcgctcct ccacgccatc
     2641 ccggggatcc acctggaggg ccccttcctg gcgccggagg acgggccgcg cggcgcccac
     2701 ccggtcagct tcatccggga cccggacttg gagttgttcc ggcagtttca ggaagccagc
     2761 aaaggtctca tccggctggt gaccttggcg ccggagcggc cgggcgccat cgagctgatc
     2821 tccaagctcc ggaaggccgg catcgtggtc gcgatcgggc actcggacgc gtcggaggca
     2881 gacatcgatc aagccgtgga ggcgggtgca cagctctcga cacacctggg gaacggcgcc
     2941 cacgctgtcc tgccacgtca tcggaattac atccagaagc agctcgccac ggatgccctc
     3001 atggcctcca tgatcgccga cggtcaccac ctcccaggct atttcgtcag aaatttcatc
     3061 cgatgcaagg ggctggatcg agtcatcctg ctcaccgatg ccctggcggc tgccgccggc
     3121 ccgccgggga cctaccgtct gggcgaggtc ctggcagagg tgggtccgga cggatacgtg
     3181 cggctgcccg gcacgccgta cctggccggc tcggccctga ccatggaccg cgcggtggag
     3241 aacctcgctc gctttgccgg cgtctcgctc tgggaggcca tcggtctggc gacccgacag
     3301 ccacgacggc tcttcccgga cctgggagcc ggcatcgcgc ccggggagcc ggccgatctc
     3361 ctcgtgctgc gggaaggacc gccactcacc gtcgtcgcca cgatcgcgca gggagagctg
     3421 gtgtaccgga gagcctagat aagcgagccg cgagccgaga gccgcgagct ggacgaagaa
     3481 acaatcagag gagggcagga tggatgcaat cgcgcagtac catgcggccg tcacggccat
     3541 gctgcagcag atcgtcgaca cccaacgtga gcccatcgcg cgcgcagccc ggtcttgcgc
     3601 cgacgccatc gcggaggacc gctacctcta cgtgatcggg acaggcggcc actcctacat
     3661 cgcctgcgag gagatgtttt ggcgcgcggg aggcctggtg ccggtctacc cgatccttga
     3721 ccctggtttt tccgtctcac acggggcctg gcggtcgatg ctcattgaac ggaccccggg
     3781 gtacgtgatt cccatcctca ggtaccacgg cctgggtaag ggcgatgtcc tgatcgtgtg
     3841 caacgcgtac gggatcaacg ccgcgacgat cgatgccgcc accgagggca aacgcctggg
     3901 ggcgacggtc atcggtgtca cgtcaacgga attcgccacc cgggtcccct cggaccatcc
     3961 cgcccgtcac cccagccgtc aaaaccttca cgagattgcc gacattttcc tgaatacctg
     4021 gatgccgtac ggtgatgcgg tggtggacat ccccggttgc ccccagaagg tcgcacccgc
     4081 gtcctcattg ggaaccgcct tcgtcctcca ctgccttgtc gccacgacgg tggaagtgct
     4141 ggtggggcgc ggcatcaccc cgcccgtgtg gcgaagcggc aatctccccg gcggggatga
     4201 ggcaaaccgg cagcacatgg acaagtattt gcggcggatc aagtttctgt aatcaggccg
     4261 agcgggcttc cggagcccac ggagacaagg agggtgccat gcgagcaagc tggaaagggt
     4321 ggatggcgct tctggccatc accgccatga gcctggccgt ctgtgaaacg cgggcggacg
     4381 ccgccaaggt caccttgaag ctggggcaca tccgcgccga tacctcaccc acgcacaagg
     4441 ccgcgctcaa gttcgccgag ctggtggggc agaagacgaa cggcgaggtg gagatcaagg
     4501 tctttcccaa cagccaactg ggcggcatcc tcgacatgtt caccggcatg aaggcgggca
     4561 gcatcgacct ggtgtacgga ggcatcaaca cgtacg
//
LOCUS       DBNGZC010000283         3281 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_903829, whole genome shotgun sequence.
ACCESSION   DBNGZC010000283 DBNGZC010000000
VERSION     DBNGZC010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_903829"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(737..1246)
                     /locus_tag="AC16G0_05230"
     CDS             complement(737..1246)
                     /locus_tag="AC16G0_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099060.1"
                     /translation="MADTTLVEPGLEGLHMSLIFEGVGNRLVFPHVDSCVALIVLCDK
                     GEMVGAHVARTEKGQELAANSGMQNAFKHLFTMLGMCPSGVKRIAFICDGGDSDDNVY
                     GQKFYDQWSGEGIELSQVDLQTKGKEGVYGSGANVYVENWGNRRVTATKMDGHTNALC
                     YVAERDLVD"
     gene            complement(1319..2647)
                     /locus_tag="AC16G0_05235"
     CDS             complement(1319..2647)
                     /locus_tag="AC16G0_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099061.1"
                     /translation="MLKHGAEAAARSHPVSRRGFLKGTVVIGSALSGIPGILRAGQAP
                     AYPKGTKLHLLQWLNFVPAGDKIFLAQAEEFGRQMGVEVQVERIGQNDVRTRAAAAIE
                     AQSGPDIVLLMHNQPLLFRGGLADVTEVAEAIAKEQAGFYDLPRANVVYEGRWMAVPG
                     FGSTSTWNYREDWLREAGFSKFPETWDQLREVGKKFKAAGHPIGQAFGHSENDPNDYC
                     YNLLWGFGGMEVAEDGKTVVLDQKWTLEALQYNLALWKDAMDEGGLSWDDASNNRAFL
                     AGSLGMTVNGPSIYIGAKDKFPEIAKAMNHALNPLGPAGRFYRVPTWSNAVMKHSKNQ
                     PLAKEFIRWVMEKPQYGKWFEVMEGFAVPPTKAWYDHPLWTSDPKLTVLRDVITFARH
                     VGYAGPPGPKASEALAKFVIVDMFAKTLQGTSPSEALKWAAGELRRIYSA"
     gene            complement(3067..>3281)
                     /locus_tag="AC16G0_05240"
     CDS             complement(3067..>3281)
                     /locus_tag="AC16G0_05240"
                     /inference="COORDINATES: protein motif:HMM:NF053320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF8156 family protein"
                     /protein_id="HIR5099062.1"
                     /translation="ALDQEGREAFDRLFAAAKFHLPAMIYASRPVSLEAIMMSILLEQ
                     QKALTQLQEATSSLGAGMHPSPDPAA"
BASE COUNT          592 a         1088 c         1001 g          600 t
ORIGIN      
        1 acgcgcagcc cgccacgggc ttatatccga aatcgcatcc agctaggccc caggctgcca
       61 atcctgagga aggccactgt atcaagataa tgcagaagtg cactcagctt gtcggggtca
      121 tgcatggttt ccgcgaaatg tcccattgag gcgccccgcg catgggtaat cccccgacct
      181 cgaagcccgt agcagacccc gcacaccgaa ctccgggaac gaagatgaaa aagcccactg
      241 cggagcgagt ctccgagtgg gccgtgggtc gccctgacag cgagtaatgc gagaccttgt
      301 tgatgaagtg gagtggcttc gcgtcggctg cggcgacggc cgtagcgacc ccgagagcga
      361 gacgccgagc tgccgcgccg ccccggcacg ggagaggcgg aagcgggtca ccagatcgac
      421 ggcgaggtgc cggcatcctg ccgtgacgcg gccctgcgaa cgagccggcc tgcgcgagta
      481 ccgtcgcccg atcctcccta tcggcaacgc tgtgcccgcc gagcggcgag ccggttgtgc
      541 agccgttcca cttcgtcaac aacttcccgc atgagaggcc cgccatccaa actgcattcg
      601 aggagaagac tgcgccccaa tacaggcaat agcccaactg gccaatcgtc agtcgccgat
      661 cagctatcgg caatcggcaa tcgccagtcg tgacgctcca gcttccaccc ctctcgctct
      721 caggttctcc gagccgtcag tccaccagat cgcgttccgc cacatagcac aaggcgttcg
      781 tgtgcccgtc catcttggtg gcggtgaccc tccggttccc ccaattctcg acgtagacgt
      841 tggcgccgga accgtaaacg ccctctttgc ctttggtctg gaggtccacc tgtgagagct
      901 cgatgccctc tccggaccac tggtcataga acttctggcc gtacacattg tcgtcgctgt
      961 cgcctccatc gcagatgaag gcgatccgct tgacccccga agggcacatg ccgagcatgg
     1021 tgaacaagtg cttgaatgcg ttctgcatcc ccgagttcgc cgccagctcc tggcccttct
     1081 ctgtcctggc cacgtgcgct cctaccattt cccccttgtc gcagagcacg atgagggcga
     1141 cgcaactgtc aacgtgcgga aacaccaggc ggttgcccac tccctcgaaa atcaggctca
     1201 tgtgaagccc ctccaggccc ggttcgacga gtgtggtatc ggccatgatt gactcctttc
     1261 agatgacgcg agatggtcag gttgagcccg acggccacgc gccgctgctg ggtcggtctc
     1321 aagcgctata tatcctccgc aactcccccg ccgcccattt gagtgcctct gacggcgagg
     1381 tcccctggag agtcttggcg aacatatcca cgatcacgaa cttggccaac gcctcggagg
     1441 ctttcgggcc gggaggaccc gcgtagccga catgccgtgc gaacgtgatc acgtcccgga
     1501 gcacggtcaa tttcggatcc gacgtccaaa gggggtggtc gtaccaggcc ttcgtgggcg
     1561 gcaccgcgaa gccctccatc acctcgaacc atttgccgta ctgcggtttc tccatgaccc
     1621 agcggatgaa ctccttcgcc aatggctgat tcttggagtg cttcatgacg gcattgctcc
     1681 acgtcgggac ccgatagaag cggcctgccg gacccagcgg gttcagcgcg tggttcatgg
     1741 ccttggcgat ttcggggaac ttgtccttcg ccccgatgta tatgctgggc ccgttcaccg
     1801 tcatcccgag gctgcccgcg aggaaggccc ggttgttgct ggcgtcatcc caggagagcc
     1861 ccccctcgtc catcgcatcc ttccagagcg ccaggttata ctgcagggcc tccagagtcc
     1921 atttctggtc caggacgacg gtcttgccgt cctcggccac ctccatcccg ccgaagcccc
     1981 agagcaagtt gtagcagtag tcattcgggt cattctcgct gtggccgaac gcctggccga
     2041 tcgggtgccc ggcggccttg aatttcttcc ccacctcccg caactggtcc caggtctcgg
     2101 ggaacttgct gaatccagcc tcccgcagcc aatcttcccg gtagttccag gtcgaggtgc
     2161 tgccgaatcc ggggaccgcc atccagcggc cctcgtagac cacgttggcc cgtggcaggt
     2221 cgtagaagcc tgcctgctcc ttggcgatcg cctcggcgac ctcggtcaca tcggcgagtc
     2281 cgccgcgaaa cagcaacggc tgattgtgca tcaagaggac gatgtcagga ccggattggg
     2341 cctcgatcgc agcggcggcc cgggtccgca cgtcgttttg accaatccgc tccacctgga
     2401 cctccacccc catctgccgg ccgaactctt cggcctgggc caagaagatc ttgtctcccg
     2461 ccggcacgaa gttcagccat tgaagcagat ggagcttcgt gcccttcgga taggcggggg
     2521 cctgtccggc ccggaggatg cccgggattc cgctcagggc cgatccgatc acgaccgtcc
     2581 ccttcaagaa tccgcgccga gacaccgggt gtgacctcgc ggcggcttcc gcaccatgtt
     2641 tcagcatgac ccttctcctt tctcccactt gcctaggccc cgtttgcccg cgggccgccc
     2701 cccgagcagg gagaagagag tccacgcccg ggacaggcga cgggaccgtg cttaggggga
     2761 atgaaagcac accggggggc cgaaaaggtc ggtgcggcgg cgagggccga gcccgtgagc
     2821 cagtgtgagt ggcgcacgat gcctcccggc tggcgtacgc cacagaggca tacgagtgca
     2881 ggagagagaa cagattttgc aagtggtaca ggggccgaag gcggccccga agagcgcatc
     2941 agcagagggt cgctcaaggg gagatgagga acaaccggca agagcctact gccggggagg
     3001 ggcatgtgtc aagcgatctt tcgagtggat ctctggcttc gggggtggcc cttcccctgc
     3061 cactgatcag gcggcgggat cgggcgacgg gtgcattcct gccccgagcg aggaagtggc
     3121 ctcctgcaat tgggtgaggg ccttttgctg ttccagaagg atgctcatca tgatcgcctc
     3181 cagcgacacc gggcgggagg cgtagatcat ggctggcagg tggaacttcg cagcggcgaa
     3241 gagccggtca aaggcctcgc gcccctcctg gtccagggcg t
//
LOCUS       DBNGZC010000284         3831 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1882622, whole genome shotgun sequence.
ACCESSION   DBNGZC010000284 DBNGZC010000000
VERSION     DBNGZC010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1882622"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..802
                     /locus_tag="AC16G0_05245"
     CDS             5..802
                     /locus_tag="AC16G0_05245"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5099063.1"
                     /translation="MKRRLEAKVAIVTGVGSRAAGIGNGRAAAILFAREGAKVLIVDN
                     KPSAAKETLEAIAGEGGEAEVFIGDVSRAADCRAMVEEAVRRWRRLDILDNNVGIEGQ
                     GSVVSVGEAEWDQVMTVNVKSMMLTGKYAVPAMAAGGGGAIINISSISALRPRGLTPY
                     STSKGAVIALTRAMAVDHAKDGIRVNCIAPGPMYTPMVYAAGMSEEARERRRRASPLS
                     IEGTGWDIGYAALFLASEEARYITGVVLPVDGGITLTTPPRGYEGTP"
     gene            1127..2410
                     /locus_tag="AC16G0_05250"
     CDS             1127..2410
                     /locus_tag="AC16G0_05250"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099064.1"
                     /translation="MDRKVDLLAEEYRRGKVTRRDFLRLAAEITGGAAAASILSTTPR
                     PAAAAASGPGIKLGAVFPLTGPWAENGNNSLHGVQLAVEHVNAQGGIKSMGGARLEVV
                     QGDTQADPALAASVTRRLVGVDKVTALFGCYLSSYTLTASTEAEKAGIPMFTNSFLDT
                     LTERGYKYFFQLPAKATAMGTAAVSYFKEAAEASGRKPKMFANVSDDNANTQKQARTI
                     SEIATKYGFQQLFLDFYTGGITDASAIVNKVRSSKAEFMFINGPTPDVILIVRTLRNV
                     GWKKPIIGTGGGGSLTRGYGEALKKDADGSYGIGAWNWDLPYPRMKEINGEFTQRFKE
                     PFIPQEAGEMYAAVWIVKEALENVKAADPEKIRNYVAATTFKEPPASLMPGNAIKFDQ
                     TGWNVNSIPIMIEWKDSMPYTVWPSQVRSRPAFFE"
     gene            2728..3603
                     /locus_tag="AC16G0_05255"
     CDS             2728..3603
                     /locus_tag="AC16G0_05255"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5099065.1"
                     /translation="MTQFLQALLSGILVGGVLGLVSMGLSLVFGVVRIVNFAHGELVM
                     LGMYGTYLLYTGMTIPPPFSMLIVAPLLFVFGLLLYRGLFEPVVLGSTELLPQLSLTV
                     GISFALQTIAQWVFSPTQRSIQMSWATTYYNVGEVFVNQAQLIAFFVSLLVSFLLWIV
                     LARTDVGRAMRAIVDDREVAQMMGINSRRIYAIAVGTSAALAAVGGNILMTYYPTFPH
                     VGLQFLPLAFVAVVMGGMGNVLGAFVGGIIVGIVQQVTGVYVAYQLQNAGLMLVFIAV
                     LLLRPSGLFGKEGSL"
     gene            3600..>3831
                     /locus_tag="AC16G0_05260"
     CDS             3600..>3831
                     /locus_tag="AC16G0_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005463192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5099066.1"
                     /translation="MKWDRDLRVGLVLLLVLASLPLWALTGSYYLGIGITALTFVALA
                     LAWNIISGIGGQLSMGHGAYFGLGAYTSSLLLV"
BASE COUNT          704 a         1110 c         1277 g          740 t
ORIGIN      
        1 ggcattgaag cgtcggcttg aggcaaaggt ggcgattgtt accggggtgg gatcccgagc
       61 ggccgggatc gggaacggcc gagcggcggc gatcctgttc gcacgtgaag gggcgaaggt
      121 cctgatcgtg gataataaac cttccgcggc gaaggaaacc ctggaggcca tcgccgggga
      181 ggggggcgag gcagaagtct tcatcggtga tgtcagccgc gcggcagact gccgggccat
      241 ggtggaagag gccgtgcgcc gctggcggcg gctggacatc ctggataaca acgtaggcat
      301 cgagggtcaa gggagcgtcg tgtcggtcgg ggaagccgag tgggatcagg tgatgaccgt
      361 caacgtgaag agcatgatgc tgaccggcaa gtatgccgtg ccggcgatgg ccgcgggcgg
      421 cgggggggcg atcatcaata tctcctccat cagcgccttg cgcccgcggg ggttgacgcc
      481 gtactccacc tccaaggggg cggtgatcgc gctcacgcgc gccatggccg tcgaccatgc
      541 gaaggacggc attcgcgtga attgcatcgc gccggggccc atgtacaccc ccatggtgta
      601 cgccgcggga atgagcgaag aagcgcggga gcgccgccgg cgggcctccc cgctgagcat
      661 cgaggggacc ggctgggaca tcggctacgc cgcgctgttc ctcgcttccg aggaagcgcg
      721 ctacatcacc ggcgtcgtgc tgccggtgga cggcggcatc acactgacca cccctccgcg
      781 cggctacgag gggactccct agagtgagac ttcgcgagct accgaaatgc aatggatcga
      841 gtgcaccgag ctacaggtat gtcctgcaaa gaggctgaca agatggagaa ggcgtcagcc
      901 tcgggatgca cgttgaaccg attcacgcgg cggaagggaa gtcccgcgga caattggagg
      961 agcatgcatc tcgcacatcc acggggaacg ggggtccacg tccgaaagga gggatctgcc
     1021 aagggtgctc tccgttgcgt tcagggagcg gttgtacgct gatgacagat gtctcctgca
     1081 tgagctggcg cgctggacca tttctcaaac tggagggagg ggaagcatgg atcgcaaagt
     1141 cgatctcctg gcggaagagt acaggcgtgg gaaggttact cgccgagatt tcttacgact
     1201 cgcggcggag attacggggg gggctgcggc tgcttcgatc ctgtctacga ccccgaggcc
     1261 ggccgctgcg gccgcgtcgg gcccggggat caaactcggg gccgttttcc ccctgacggg
     1321 accctgggcc gaaaacggga ataattccct tcacggcgtc cagttggctg tggagcacgt
     1381 caacgcccag ggagggatca agtcgatggg gggcgccaga ctcgaggtcg tccagggcga
     1441 tacccaagcc gatccggcgc tcgccgcgtc ggtcactcgt cggttggtcg gggtggacaa
     1501 agtgactgcg ctcttcggct gctacctgtc cagctatacc ttgaccgcca gcaccgaggc
     1561 ggagaaggcc ggcattccga tgttcaccaa ctccttcctg gatacgctca ccgaacgagg
     1621 atacaaatat ttcttccaat tgcccgccaa ggcgaccgcc atgggtaccg ccgccgtgag
     1681 ctatttcaag gaagcggctg aagcctccgg gcgcaagccg aagatgtttg ccaatgtgtc
     1741 tgacgacaat gccaacacgc agaaacaagc cagaaccata agtgaaattg ccaccaagta
     1801 tggcttccag caactcttcc tggacttcta caccgggggc atcaccgatg catcggccat
     1861 cgtcaacaag gttcgctcca gcaaggcgga gttcatgttc atcaatggtc cgactcccga
     1921 cgtgatcctg atcgtacgga ccttgcggaa cgtgggctgg aagaaaccga tcatcggaac
     1981 aggcggcggc gggtcactga cacgggggta tggcgaggcg ctgaagaagg acgccgatgg
     2041 atcctacggc atcggggcgt ggaactggga tctgccctat cctcgaatga aagagatcaa
     2101 cggggagttc acccaacggt tcaaggagcc gttcatcccg caggaagccg gcgagatgta
     2161 cgcagccgtg tggatcgtca aggaggcttt ggagaacgtg aaggcggccg acccggagaa
     2221 gattcggaac tatgtggcgg ccaccacgtt caaagagccg ccggcctcgc tcatgccagg
     2281 caacgccatc aagtttgacc agacgggctg gaatgtcaat tccatcccca tcatgatcga
     2341 gtggaaggat tcgatgccct ataccgtgtg gccatcccag gtgcgctcga gaccggcgtt
     2401 ctttgagtaa acgtcaaggg ggggacgcag tcggaacagg ctcgtggtca ggtctcggtc
     2461 tcgggcggga gacaaggagt tgcctcttgc ggatctgggc gagggcaccg gttcttgcct
     2521 ggcgatctcc gcgacatcga gccctccacc agggctgcgc gtgcccgagg gggctcgtgg
     2581 gtttgagggg caccctccgg aatggcgcct ggcgtggccg ggtacccctg gcttcacccg
     2641 ccaggttggg tgcaaaggcc taccagccag tgaggactta tcgccgcgca catatgttgt
     2701 tggatgagaa atccaaggag aacccacatg acccagttcc tccaagccct cctgagcggc
     2761 attctcgtgg gcggcgtcct tggactcgtg agcatgggtc tcagcctggt gtttggcgtg
     2821 gtccggatcg tcaatttcgc ccacggcgaa ttggtcatgc tgggtatgta cggcacctac
     2881 ctgttgtaca ccggcatgac cattcctccg cccttctcga tgttgatcgt cgcgcctctg
     2941 ctgttcgtat ttggcttgct cctctatcgg ggactcttcg agccagtggt gctcggatcg
     3001 acggagctcc ttccccagtt gtcgctcacg gtgggaatct cctttgccct gcagacgatc
     3061 gcgcagtggg tcttctcgcc gacccagcgg tccatccaga tgtcctgggc cacgacgtat
     3121 tacaatgtgg gcgaggtgtt cgtcaaccaa gcgcaactga tcgccttctt cgtatccctc
     3181 ctggtctctt ttctgctgtg gatagttctg gcccgtaccg acgtcggccg cgccatgcga
     3241 gccatcgtgg atgatcggga ggtcgcccag atgatgggta tcaacagccg ccggatctat
     3301 gccatcgccg tgggaacttc ggctgccctg gcggcggtag gcggtaacat cctcatgacg
     3361 tactacccca ccttccccca cgtcggcctg cagttcctgc cgctggcgtt tgtggccgtg
     3421 gtcatgggcg gtatgggcaa cgtgctgggc gctttcgtcg ggggcatcat cgtgggcata
     3481 gtgcagcagg tgacaggagt gtacgtggcc taccaactgc agaacgccgg gttgatgctg
     3541 gtgttcattg cggtgctgct gttgcggccc tccgggttgt ttggaaagga gggctcgcta
     3601 tgaaatggga tcgggatctc agggtcgggc tggtgctgct cctggtgctg gcctccttgc
     3661 ccctatgggc gctgacgggc tcgtattacc tcgggatcgg gatcaccgcg ctgacctttg
     3721 tcgcgctggc cttggcctgg aatatcatct cgggcattgg cggtcagctc tcgatgggac
     3781 acggggccta cttcggcctg ggggcctaca ccagctccct gctgctggtc c
//
LOCUS       DBNGZC010000285         2986 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_121314, whole genome shotgun sequence.
ACCESSION   DBNGZC010000285 DBNGZC010000000
VERSION     DBNGZC010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_121314"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2260)
                     /locus_tag="AC16G0_05265"
     CDS             complement(<1..2260)
                     /locus_tag="AC16G0_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5099067.1"
                     /translation="MQWLAEVSVKRPVFASVLVLSLAVVGVFAYFRLGVDLFPKVDIP
                     VITVTTRQSGAAPEEIETEITDKIERAVNTIAGIDELRSVSTEGVSQVFIQFTLEKNV
                     DVAAQEVRDKINQILPDLPQDIDQPVVDKMDPDAAPVIYVALSGDRPVRDISEFADKT
                     LRREIESISGVGQVTLVGSRLRQVNLWLDPARLRAYSLTATEVTRAVANQNLMLPGGN
                     LTQGSRELTVRMKGRVNSVPEFDGIVLASRAGTQITLAQVGHAEDGVEEAETAGNVDG
                     RSAVILAVRRQSGTNTVAVADEVTKRLNAMRTRLPAGYKMEITRDQSDFIKDSVKNVQ
                     EHLIVGAILAALVVLLFLRNWRSTLIAAIAIPTSIISTFALMYAMGFTLNMLTLLALT
                     LAVGIVIDDAIVVLENIYRYMEEKGLSPVAAAIQGTREIGLAVLSTTLSLVAVFLPVA
                     FMGGIIGRFMYSFGLTMAFSILVSLLVAFTVTPMLSSRWLSAKKTGGRTSKDSRFFRI
                     LDNGYEQLLTWVMAHRWVIVTASLVALVSILPLFMLVGKDFLPKDDESQFEVSIRTAE
                     GTTLEQTELIATRIGREMKALSGVAYTVVLVGNDDRRTANLATVFVKLVDIPKRKSTQ
                     FDIMEQVRTTVLPKFAAEKLRTSVSQVAAISGGGMANKEISFYVSGPDLQKLAEYTTR
                     LTTALAKVPGVVDVDTSLILGKPEMAVNLDRKKAAELGVQVADVADTLAVMVGGRKIS
                     TYNENGEQYEVHA"
     gene            complement(2263..>2986)
                     /locus_tag="AC16G0_05270"
     CDS             complement(2263..>2986)
                     /locus_tag="AC16G0_05270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5099068.1"
                     /translation="SRAEHDLRRADYLAAKAQFETSVNQTRQLYQSLLAQKVRVNMGR
                     KAVEDTIIRAPWSGLIAEKAVNVGRYMKKGDRIATIVRVDPLRIELAIPEGAVAAVRR
                     GQKVSFTVQSYPDRQFEGTIAYIGPAVRAESRALVVEAIVPNPKAVLQPGLFATAHIE
                     LPAARASVMIPSAAVRNEGGVFKLFVAKGDRAEVRIVQVGREADERLEILRGLAPGES
                     VVNPQTASLADGTAIAVAREAK"
BASE COUNT          488 a          980 c          997 g          521 t
ORIGIN      
        1 gggcgtggac ctcgtactgt tccccgttct cgttgtaggt ggagatcttt cgcccgccga
       61 ccatgacggc cagcgtgtcg gccacgtccg ccacctgcac gcccagctcc gcggccttct
      121 tccggtccag gttcaccgcc atctccggct tgcccaggat caggctggtg tccacgtcca
      181 cgacccctgg aaccttggcg agcgccgtgg tcaggcgagt cgtgtattcc gccagcttct
      241 gcaggtcggg gccgctgaca tagaacgaga tctccttgtt ggccatgccg ccgcccgaga
      301 tggcggccac ctgggagacg ctggtgcgaa gcttctcggc cgcaaacttc ggcagcacgg
      361 tcgtgcggac ctgttccatg atgtcgaact gagtgctttt ccgcttggga atgtcgacca
      421 gcttgacgaa aacggtggcc aggttggccg tgcgccggtc gtcgtttccg accaggacca
      481 cggtgtaggc cacccccgag agcgccttca tctcgcgccc gatccgggtg gcgatcagct
      541 cggtctgctc cagggtggtg ccctcggcgg tgcggatgga gacctcgaac tgcgactcgt
      601 cgtctttggg caggaagtcc ttgcccacca gcatgaagag aggaaggatc gagaccaggg
      661 cgaccagcga ggccgtgacg atcacccagc ggtgggccat gacccaggtg aggagctgtt
      721 cgtatccgtt gtccaggatg cggaagaacc gggagtcctt ggatgtgcgc cctcccgtct
      781 tcttcgccga gagccagcgg ctggacagca tgggggtaac ggtgaaggcc accagcagcg
      841 ataccaggat ggagaaggcc atggtcagac cgaagctgta catgaaacgg ccaatgatgc
      901 cgcccatgaa ggctaccgga aggaagacgg cgacgagcga cagcgtcgtc gacaggaccg
      961 ccagaccgat ctcccgcgtg ccctggatgg ccgctgccac cggggagagg cctttctcct
     1021 ccatgtagcg gtagatgttt tccagcacca cgatggcgtc gtcgatgacg atgcccacgg
     1081 caagggtgag cgccagaagc gtcagcatgt tcagcgtgaa gcccatggcg tacatgaggg
     1141 caaaggtgct gatgatggag gtcgggatgg cgatggccgc gatcagggtt gagcgccagt
     1201 tccgcaggaa gagcaatacg accagagccg ccaggatcgc cccgacgatc aaatgctctt
     1261 ggacgttctt gacggaatcc ttgatgaagt cggactggtc ccgggtgatc tccatcttgt
     1321 agccggccgg caggcgcgtc cgcatggcgt tgagccgctt cgtcacctcg tccgccacgg
     1381 cgaccgtatt ggttcccgac tgccggcgga ccgccaggat caccgccgac ctgccgtcca
     1441 cgttcccggc ggtctcggcc tcctcgacgc cgtcctccgc atggcccacc tgggccagcg
     1501 tgatctgggt gccggcccgg ctggccagca cgatcccgtc gaattccggc acgctgttca
     1561 cccgcccctt catccggacg gtcagctcgc gggagccctg cgtcaggttt ccgccgggga
     1621 gcatgaggtt ctggttggcc acggcgcggg tgacctccgt ggcggtcagg ctgtaggcgc
     1681 gcaggcgcgc gggatccagc cagaggttga cctggcgcag gcggctcccg accagggtca
     1741 cctgtcccac acccgagatg gactcgatct cgcggcgaag ggtcttgtcc gcaaactcgc
     1801 tgatgtcacg gacgggtcgg tcgcccgaga gcgccacgta gatcaccggg gcggcgtcag
     1861 ggtccatctt atccaccacg ggctggtcga tatcttgggg gaggtcgggc aagatctggt
     1921 tgatcttgtc gcgcacctct tgcgccgcca cgtccacgtt cttctcgagc gtgaactgga
     1981 tgaagacctg ggagacgccc tcggtggaaa cggagcgaag ctcgtcgatg cccgcgatgg
     2041 tgttcaccgc ccgctcgatc ttgtcggtga tctcggtctc gatctcctcc ggcgccgcgc
     2101 cggactgccg cgtcgtcacg gtgatcacgg ggatgtcgac cttggggaac agatccacgc
     2161 ccagccggaa gtacgcgaag acgccgacca ccgcgaggga gaggacgagg accgaggcga
     2221 agacggggcg tttgacagag acttcagcca gccactgcat gactacttcg cctcccttgc
     2281 gaccgcgatc gccgtcccgt ccgcgaggct ggccgtctgc ggattcacca ccgactcccc
     2341 gggcgcaagg ccgcggagga tctccaggcg ctcgtccgcc tcgcggccca cctggacgat
     2401 ccggacctcg gcccggtcgc ccttggcgac gaacagcttg aacacgcccc cctcgttccg
     2461 cacggccgcg gacgggatca tcacgctggc gcgcgcggcc ggcaactcga tgtgcgcggt
     2521 ggcgaagagg cccggctgga gcacggcctt ggggttcggg acgatggcct cgacgaccag
     2581 ggcacgggac tcggcccgca cggccggacc gatgtaggcg atggttccct cgaactgccg
     2641 gtcgggatag ctctggaccg tgaaggacac cttctgtccc cggcgcaccg cggccacggc
     2701 cccctcgggg atggcgagct cgatccggag cggatccacc cggacgatcg tcgcaatccg
     2761 gtcacccttc ttcatgtagc ggccgacgtt cacggccttc tccgcgatca ggccgctcca
     2821 gggggcgcgg atgatcgtat cctccaccgc cttgcgcccc atgttgacac ggaccttctg
     2881 ggccaggagg gattggtaga gctgtcgcgt ttggttgacg ctggtctcga actgggcctt
     2941 ggccgccagg tagtcggccc ggcggagatc atgctcggcc cgggag
//
LOCUS       DBNGZC010000286         2038 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2370712, whole genome shotgun sequence.
ACCESSION   DBNGZC010000286 DBNGZC010000000
VERSION     DBNGZC010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2370712"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..951
                     /locus_tag="AC16G0_05275"
     CDS             289..951
                     /locus_tag="AC16G0_05275"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR5099069.1"
                     /translation="MLGQELRDYFTRCAIDLPFRYREATEVAYRPGVGWTRDLSGGGA
                     WVELPERVAPPTLLDVSLRAPGESLQLPAQVVWGCPEPHEQSRLHGLRFTHITPAQRQ
                     RLGGLLTHQKSRDVERLYCTLAATCRSKTADFGPLPCETRDVSSGGVALRLPTPVSPG
                     TPLQVTILTPFGAVTADAQVVWAEPSDALPRGAPCRHGLRFLHIEPSSELPLKLLLAG
                     LR"
     gene            complement(1309..1527)
                     /locus_tag="AC16G0_05280"
     CDS             complement(1309..1527)
                     /locus_tag="AC16G0_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099070.1"
                     /translation="MVVVVVGLAWTLANSTATAQAQGPTNGPKAIRANQFVLVYETGK
                     PRVALDVDKVGPFLDLLDENGKVIWYQP"
     gene            complement(1773..1979)
                     /locus_tag="AC16G0_05285"
     CDS             complement(1773..1979)
                     /locus_tag="AC16G0_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099071.1"
                     /translation="MIEVEGDGIYRKHRIHTSQLFSGVWVASVVHLGAQGPLTETVPG
                     DYRTHEEAVEAAKRHSEQKEEQLK"
BASE COUNT          303 a          715 c          632 g          388 t
ORIGIN      
        1 cgcgtgctcc ggacattgcg ccatcgtgcg gcctcggcgc catccgtcca agcgggtgtg
       61 gccacatcgc aggcggcgag cccgtcgttc tggttgcggg tggcccatgt gccccaaccg
      121 atggcgccgg acccgcccag ttttcactga gtcctggccc tgacctccgg gtccctgtcg
      181 cggcccgcgt cgccccatgg ccagagaggt ggagttcggt gaggaggagg tctcccgcac
      241 ttggggggcc tgaatcatcc ccgcgcattg catcaaggag gggagagcat gttgggacaa
      301 gaactgcggg actacttcac gcgatgtgcg attgacctgc ccttccgcta ccgcgaggcg
      361 acggaggtcg cctaccgccc tggtgtcggt tggacccggg acctgagtgg aggcggcgcc
      421 tgggtcgaac tgcccgagcg ggtggcaccg ccgaccctcc tagacgtgag cctccgcgcg
      481 cccggggagt cgctgcaact gcccgcgcag gtggtctggg gctgccccga gccacacgag
      541 caatcccggc tccacggatt gcgcttcacc cacatcacac cggcgcagcg acagcgcttg
      601 ggggggctgc tcacccacca gaaatcccgt gacgtcgagc gcctctattg taccttggcc
      661 gccacctgtc ggagcaagac ggccgatttt ggcccgctcc cctgcgagac ccgggatgtc
      721 tcctccggcg gggtcgccct tcgcctgccg acgccggtgt cgccgggcac gcctctgcag
      781 gtgacgatcc tcaccccctt tggtgccgtc acggctgacg cccaagtcgt ctgggccgag
      841 ccatcggatg ctctgccccg gggcgcccca tgccgacacg gcctgcggtt tcttcacatc
      901 gagccgtcga gcgagctgcc cctgaaattg ctgctcgctg gactgcggta ggcagaggca
      961 atcatcgcag aatcagcccg acgaccgtca cgaggcccct gaagacaggc cagaccctgt
     1021 tgggggtgag gggcgttcgc gcggcgatcc ctaacctccg gctaagcggg agctagcgca
     1081 tcgattcgct gccgcaccac caccatgagg gcgagctgac ggccctccac aagtcatccc
     1141 gcaaccatac cgtcgtctgg gttacgcaca ttggctcaac ccggtcgctg tcgtcctact
     1201 ccccagatag ccggaggcgg cggcgaaagg cggccaggcc cgtggtcagg ctgactgcgg
     1261 acgtggtctt cccgattctg cccttgcggt tgaccagggc aagcaacctc atggttgata
     1321 ccagatcacc ttgccgttct cgtcgagcag gtcaaggaac ggtccaacct tgtccacgtc
     1381 cagcgcgacg cggggcttgc cggtctcata caccagaacg aactgattgg cccggatcgc
     1441 ttttggcccg ttggtcggcc cctgggcctg ggcggtcgct gtgctgttcg ccagtgtcca
     1501 ggccaggccc accacgacga ccaccacccc cagggcgacc agcagtcagc gggtgcggcg
     1561 cattccggcg agcggtttcc gctctagcct attcagacgg tctttggtgc tcatcgtctc
     1621 tgcctttctc gggtggagcg ggagtgcacg ccacgccctt ggcgagtctg cagctcgcgc
     1681 gagggttctt ccagtgtctt ccggcctcac ggccgtgtcc tccttcctcc ggagccccca
     1741 gcaggtgcca ctgacgcggg ggcaacttcc ttctacttta gctgctcctc tttctgctca
     1801 ctgtgtcgct tggcggcctc cacggcttct tcatgggttc ggtaatctcc agggacagtc
     1861 tcggtcaagg ggccttgcgc ccccaagtgc accacggagg ctacccagac gccgctgaac
     1921 aactgtgacg tgtggatccg gtgtttcctg tatatgccgt ccccctctac ctcgatcacg
     1981 ctcgctccgt tggattgcgg attcctttag cagcttcccc agcttcaccc ccgatcag
//
LOCUS       DBNGZC010000287         1435 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2469431, whole genome shotgun sequence.
ACCESSION   DBNGZC010000287 DBNGZC010000000
VERSION     DBNGZC010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2469431"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="AC16G0_05290"
     CDS             complement(<1..862)
                     /locus_tag="AC16G0_05290"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR5099072.1"
                     /translation="MKAGAYDFLPKPFDPKHLEIVVRKALERTTLVESNRLLRDTLAA
                     RTSEVLGESPAIRQAVEIARRAADTNTTVLLLGESGTGKEIFAQAIHRWSPRSERPLV
                     VVNCVALSEHLLESELFGHEKGAFTGAHQTKRGKFELAQGSTVFLDEIGDMASSLQVK
                     LLRVLQERTIERVGGTRPIRADVRIIAATNRDLDAAVKEGRFREDLFYRLNVVRILLP
                     PLRQRREDIALLAHHFVAKYAAETKRPVQGIAQSALDALTAHDWPGNVRELANMIERA
                     VVLCGRTQIQM"
     gene            complement(863..1147)
                     /locus_tag="AC16G0_05295"
                     /pseudo
     CDS             complement(863..1147)
                     /locus_tag="AC16G0_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
BASE COUNT          233 a          439 c          497 g          266 t
ORIGIN      
        1 ccatttggat ctgggtcctg ccgcagagca ccaccgcccg ctcgatcatg ttcgctagct
       61 cacgcacgtt ccccggccag tcgtgggcgg tgagggcgtc caaggccgac tgagcgattc
      121 cctgtactgg ccgcttggtc tcggccgcgt acttggcgac aaagtggtgc gcgaggagcg
      181 cgatgtcctc ccggcgctgg cggagcgggg ggaggaggat ccggaccaca ttcaggcggt
      241 agaacaggtc ctcgcggaag cggccttcct tgaccgcggc atccaggtcc cggttggttg
      301 ccgcgatgat gcggacgtcc gcccgaatcg ggcgggttcc accgacgcgc tcgatggtgc
      361 gctcctgcag cacgcggagc agcttcacct ggaggctcga agccatgtcg ccgatctcgt
      421 ccaggaagac ggtgctcccc tgggccaact cgaacttacc ccgcttggtt tggtgcgcgc
      481 cggtaaacgc gcctttctcg tggccgaata gctcgctctc gaggagatgc tccgacaggg
      541 cgacgcagtt gacgacgacg agggggcgct cgctccgggg gctccagcgg tggatcgcct
      601 gggcgaagat ctccttacct gtgccgctct cgccgagcag cagcaccgtg gtgttggtgt
      661 ccgccgcgcg gcgggcgatc tcgaccgcct ggcggatggc cggactctcg cccaggactt
      721 ccgacgtccg ggcggccagg gtgtcccgca gcaggcggtt ggattccacc agggtggtcc
      781 gctcgagggc cttgcggacg acgatctcga gatgttttgg gtcgaagggc ttgggaagaa
      841 aatcgtaggc cccggccttc atcgcatcga ccgcagcctc gacggatccg tgcgcggtga
      901 tgacgatgac cgggatatcg attccggcct ggtggagggc ctggagcacc tagagcccgc
      961 tcattctggg catctgcagg tcgaggagca cgagcgaagg ggggtcggac tggatgcgct
     1021 cgagcgcttg ctggccatcg ctggccgttg ctgtttcgca cccgagggtc ttgaggcgca
     1081 cctcgatcag gcgccgtgcg gtaggatcgt catccacgat caggactgtg gcgccagatg
     1141 cttccatggg gttctctcgg agagtgcttc cctgaccaac acgacgcgtc tccggcgccg
     1201 cgtggccggg cgagaaggaa gaagtccgaa cgaaagattc aatacggata agaacggtgt
     1261 tctaaagatg ccccattggg caagtggtgt caacacccat ttcgagtcaa ttgcggtcgt
     1321 gccgggtggg cgctgtccgc cagaaggggc ctcgaggagt tgatcgttca cgggacaacg
     1381 ataccgcatc cggtgatggc gcgttgtact cgcctcatgc aaggtcgccc gtcct
//
LOCUS       DBNGZC010000288         3262 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_512139, whole genome shotgun sequence.
ACCESSION   DBNGZC010000288 DBNGZC010000000
VERSION     DBNGZC010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_512139"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="AC16G0_05300"
     CDS             <1..950
                     /locus_tag="AC16G0_05300"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF037628.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR5099073.1"
                     /translation="VGGISLAGVGVGHVGFGLAPRINAAGRLDDAALAVRLLLTRDPL
                     EAQNLAQELDRQNRERQELEGSILEAAVAQAEAEFDRDRDRALVLASAKWHPGVIGIV
                     AARLVERFGRPTALIGIQGDHARGSARTAAGWHIADALHRCEDLLLDYGGHRAAGGFS
                     LRVDQIGVFRERFLSLAAQDLADEDFVPTLTADAEVALESVDLALADNLARLAPHGVG
                     NPEPTLIARGLQVMRSARLVGRNHLKMKVRQSPRGQVVDSIGFNLGSFVEVLGQPTAP
                     HIDLAFTPERNTWNGRDALQLRVKDLHLLGETAAADQGD"
     gene            1329..1910
                     /locus_tag="AC16G0_05305"
     CDS             1329..1910
                     /locus_tag="AC16G0_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HIR5099074.1"
                     /translation="MFRESAAVKLRFVEASSGRIEAVAHEMAEVLRRGGKLLFLGNGG
                     SAADAQHLAAEFVNRFLVNRKALAALALTTDSSVLTSIGNDLGMEQVFSRQVEALARP
                     GDLVVAISTSGNSPNVLRAVEAARRLGCGTIGLTGGSGGALAGAVDEAFVVPSQETPR
                     IQETHITLGHALCALVEDLLVQSGDAPAASPGD"
     gene            2034..3035
                     /locus_tag="AC16G0_05310"
     CDS             2034..3035
                     /locus_tag="AC16G0_05310"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018701749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR5099075.1"
                     /translation="MRHCVLAFGVLALLLFASAGSTPASPNRAGVSKHRVAHRTAGWH
                     RDRASSLLPESLEIPRSGPREVNAVSAVLMDAKTGQVLYARNPDEPLPPASVTKILTA
                     LVILDRGHLDDTVVVSQEAARVGGYRLGIRRGQQLSLQDLLAAILIRSANDAAVAAAE
                     HVGHGLAGFVDLMNAKAEELGMLHSHFANPHGLDEPGHFTTARDLAVLTRVALEQPAF
                     AQLVRTRQISVTIWNPGRRGLVARARPIQTHNKLLGRVEGADGVKTGYTDGAGRCLVA
                     SASRGDQRMIAVLLNDPRRWSDAASLLEYGFEAVGSAAGVREVDRPGSLARTTVDQN"
     gene            3064..>3262
                     /locus_tag="AC16G0_05315"
     CDS             3064..>3262
                     /locus_tag="AC16G0_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004970116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR5099076.1"
                     /translation="MKRTSSSCLGCFGLIVLCLPLAAWAAEAQPAPTAPPGETYVVKK
                     GDTLWAISKGLLNDPILWPQIW"
BASE COUNT          521 a         1027 c         1156 g          558 t
ORIGIN      
        1 aggtgggcgg catctccctc gccggtgtgg gtgtagggca tgtgggattc ggcctggccc
       61 cccgcatcaa cgccgctggc aggttggatg atgcggcact ggcggttcgg ctgctgctca
      121 cccgagatcc attggaagcg cagaatttgg ctcaggagct cgaccgccag aaccgcgagc
      181 ggcaggaact cgaaggctcg attcttgagg cggccgtggc ccaggcggag gcggagttcg
      241 accgcgaccg cgatcgggcc ctggtcctgg cttccgcgaa gtggcatcca ggggtcatcg
      301 gtatcgtggc ggccaggctc gtggagcggt tcggccgtcc gacggcgctg attggcatcc
      361 agggtgacca cgcccggggt tccgcgcgta ccgctgccgg ctggcacatt gccgatgccc
      421 tgcatcgatg tgaggatctg ctcctggact atgggggaca tcgtgcggcc ggggggttct
      481 ctctccgggt ggatcagatc ggggtcttcc gggaacgatt cctttctctt gcggcccagg
      541 acttggcaga cgaggacttc gtgcccacgc tgaccgcgga cgcggaggtc gccctggaaa
      601 gcgtggacct tgccttggcc gacaacctag cccgattggc cccacacggg gtaggaaatc
      661 ccgagcctac cctgatcgcc cgcgggcttc aggtgatgcg atcagcgcga ctcgtgggtc
      721 ggaaccactt gaagatgaag gtccgccagt ctccccgggg gcaggttgtg gattccatcg
      781 gcttcaacct gggatcgttc gtcgaggttc tgggccagcc aactgcaccc catatcgatc
      841 tcgccttcac tcccgagcgt aacacctgga acggcaggga tgcccttcaa ctgcgggtga
      901 aagacctgca cctcctgggg gagaccgccg ccgccgacca gggcgactga ctcggccgcc
      961 gcgacgcgtc ctttctccaa tcctctcatt gtgctgacgt tttgtcgccc aggggtgccg
     1021 ttgcctcggc cttcttgtcg tcacgggcgt gtggcaatcg tgcgtttggc ggtgccgcgc
     1081 gggtctgggc cagaatttgc atcgcatgca ggagaggtca tttggaccgc cagacgtttg
     1141 gcagcgtgat ttttccttga caaggtcagg cccgtcgcta tagtggcgcg gacggtcaat
     1201 cccattgggg acgcggtgtt gcgacggtca ggctgcggcg ggaaaggctg aaccctggag
     1261 cggtcggggg cttcaggccg cggcaaagaa agatggaagg gcgtcccatg caggctggta
     1321 tccaccgaat gttccgtgaa agcgccgctg tgaagcttcg cttcgtcgaa gcctcgagcg
     1381 ggcgaattga agccgtggcg catgagatgg cggaggttct tcgtcgcggc gggaagctcc
     1441 tattcctggg aaacgggggg agcgcggcgg atgcccagca cctggcggcg gagttcgtga
     1501 accgcttcct ggtgaatcga aaggccctgg cggcgctggc cctgacaacg gatagctcgg
     1561 tcctcacgag catcggcaac gacctgggga tggagcaggt cttctcacga caggtggagg
     1621 cgctggcccg tccgggggat ctggtcgtgg cgatcagcac cagcggcaac tccccgaacg
     1681 tcctccgagc ggtcgaggcc gctcgacgct tgggttgcgg gacgatcggc ttgacgggcg
     1741 ggtccggggg ggccttggca ggggccgtgg acgaggcctt cgtcgtgccc agccaggaga
     1801 cgccccggat ccaggaaacc cacatcaccc tgggccacgc cttgtgcgcg ctggtcgagg
     1861 acctgctcgt ccaaagcggg gacgcgccgg cggcctcccc aggcgactga ggggataaga
     1921 ggcgaagcgg tcgagatccc gggatggcgg tgtgacaggg aaacgcaacc ccgccgtcgg
     1981 gatatccggt caacggcggg ccctggggtt cgccccatca ggagggagaa ggcgtgaggc
     2041 actgcgtcct ggcgttcggt gtgctggcac tcctcctgtt cgcttcggcc ggctcgacgc
     2101 ccgcgtcgcc aaaccgggcg ggagtctcga agcaccgggt agctcaccgg acagccggat
     2161 ggcatcggga ccgcgcctcg tccttgctcc cggaatcgct ggagattcca cggtccgggc
     2221 cgagggaggt caatgcagtc tcggccgtcc tgatggacgc caagacgggg caggtgctct
     2281 acgcccggaa cccggatgag ccgcttccac cggcgagcgt cacgaagatc ctcacggcac
     2341 tcgtgatcct ggaccgaggc cacctagatg acacggtcgt ggtcagccag gaggcggctc
     2401 gggtcggtgg gtaccggctg ggcatccggc gcggccaaca actctcgctc caggacctcc
     2461 tcgccgcgat cctgatccgc tcggccaacg acgccgccgt ggcggcggcc gagcatgtgg
     2521 ggcacggtct tgcgggcttc gtcgacctga tgaatgccaa ggcggaagag cttggcatgc
     2581 tgcacagcca cttcgccaac ccccatgggt tggacgaacc cggccacttt acgacggccc
     2641 gggacttggc ggtgctcacg cgtgtggcgc tggagcagcc ggccttcgct cagctcgtgc
     2701 ggactcgaca gatctcggtg acgatctgga atcccggtcg gcggggattg gttgctcggg
     2761 cgcgccccat ccagacccat aacaagctgc tcggacgcgt ggagggcgca gacggtgtga
     2821 agaccgggta caccgacggg gccgggcggt gtctggtggc ctctgccagc cggggcgacc
     2881 agcggatgat tgccgtgttg ctgaacgacc cgcggcgctg gtcagacgcc gcctcgctcc
     2941 tggagtacgg gttcgaggcc gtcggcagcg cagccggggt gcgggaagtc gaccgccctg
     3001 gctccctggc ccggacaacg gtcgaccaaa actagggcgc ccgaccgcca cggaggagtg
     3061 gacatgaaac gaaccagcag tagctgcctt ggctgcttcg ggctgatcgt cctgtgcctc
     3121 cccctcgccg cgtgggcggc cgaggcccag cccgctccga cggccccacc aggcgaaacc
     3181 tacgtggtga agaaggggga taccctctgg gcgatctcca agggcctcct caacgatccg
     3241 atcctttggc cgcagatctg gg
//
LOCUS       DBNGZC010000289         2992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1490931, whole genome shotgun sequence.
ACCESSION   DBNGZC010000289 DBNGZC010000000
VERSION     DBNGZC010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1490931"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..257)
                     /locus_tag="AC16G0_05320"
     CDS             complement(30..257)
                     /locus_tag="AC16G0_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HIR5099077.1"
                     /translation="MRWQDRITVTSGVRSGKPCIRGTRITVYDVLEYLAGGMTEAQIL
                     ADFPDLTAEDIRACLAFAASRERRLASSPPV"
     gene            complement(323..1090)
                     /locus_tag="AC16G0_05325"
     CDS             complement(323..1090)
                     /locus_tag="AC16G0_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012669.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR5099078.1"
                     /translation="MHPLIRHQKILALLEGHRVVPVGRLARAVRASEMTVRRDLSLLR
                     DRGLVRKVHGGAVLEVRKAEESHFSATREERVEEKQAIAKEAATLVSPGSVVALGAGT
                     TTWHVARTLADRTDLTFITNSTNIALELDRGHSNIILTGGHFRTPGDALVGPPALWTL
                     ERLQIDLLFLGANGIDLKAGYTTPVLAEVEVNLALIRKASRVVLVADHTKFGRVTLAS
                     FGPLETAHVLITDPATPRSILAAIRRRGVQVVVAAKN"
     gene            complement(1181..2125)
                     /locus_tag="AC16G0_05330"
     CDS             complement(1181..2125)
                     /locus_tag="AC16G0_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR5099079.1"
                     /translation="MTTERFRISQLPRTLQPLVAEAISSAQDAGRILKRRFRGAMQVW
                     MKGQADVVTATDLECQTLIVGRLRRRFPAHGVLAEEGLNLAPGAEWRWILDPLDGTKN
                     FARGVPSFCVSIAAEREGRVELGVVHDPIHGETFVGVHKHGVWCNERPIRVSDVATLR
                     AAFLATGLPHRVRRFVGSVSRTFGRFSARTLGVRDRGAGALDLCYVACGRFDGYWEID
                     QSPWDTAAGGLIVTEAGGRMSDFRGGGFDIYDGETVASNGKIHDQILAVLALPGGIPG
                     RHHPPVHRSTAQSQRHQGTKKFLGHPREAIDPGEVVEH"
     gene            complement(2125..>2992)
                     /locus_tag="AC16G0_05335"
     CDS             complement(2125..>2992)
                     /locus_tag="AC16G0_05335"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099080.1"
                     /translation="AHKRDIGMVFQNYAIFPHLTVFENVAYGLRARRVPPTEMGRRAA
                     EALHLVQMEALADRRPDQLSGGQQQRVALARAMAIQPRLLLMDEPLSNLDAKLRVEMR
                     TEIRRLQRELGITTIYVTHDQEEALAISERLAVMNAGHVEQVGFPWDVYQTPQSAFVA
                     QFIGTSNALTGRVVGHQGEWTHVDVGGWSLRARRSDDLADRALVVFRPEAVGVLTQGE
                     GCPEATPGVGVVISREFLGAILRYRLRLGDGSLIHLDEHKPDAARLRKEGEQIRFSLH
                     PADVMLFPIEES"
BASE COUNT          514 a         1017 c          946 g          515 t
ORIGIN      
        1 ggggctcagg ttctcgtcga atagtagctt cacaccggtg gggaactcgc cagccggcgt
       61 tcccggcttg cagcgaaggc caggcaggcg cggatgtctt ccgccgtgag gtcagggaag
      121 tcagctagga tttgcgcttc ggtcatgccc ccggcaaggt actccaagac gtcgtacacc
      181 gtaatgcggg ttccccggat acagggcttt ccgctccgaa ccccggaggt aacggtgatg
      241 cgatcttgcc agcgcatgat catgagtcta gcctaccttg ccgaacactg caagcgaccc
      301 tcccccatct tttctcttac cgtcagttct tggccgccac gactacctgg acgccgcggc
      361 ggcggatagc ggcgaggatg gaccgcggcg tggcggggtc ggtgatgagg acgtgggcgg
      421 tttcgagagg gccgaagctg gccagggtca cccggccgaa cttggtgtga tcggcgacca
      481 ggacgacgcg ggaggctttc ctgatcaagg ccaggttcac ctcgacttcg gccaggaccg
      541 gcgtggtgta gccggccttc aggtcgattc cgttcgcccc aaggaagagc aggtcgattt
      601 gcagccgctc caatgtccag agcgcggggg gacccaccag ggcgtctccc ggggtgcgga
      661 agtggccccc ggtgaggatg atgttcgagt ggccccggtc cagctccaaa gcgatgttcg
      721 tggagttggt gatgaaggtc agatccgtcc gatcagccag tgtcctggcc acatgccagg
      781 tggtcgttcc ggcccccagg gcgaccacgc ttccgggaga caccagggtc gccgcctcct
      841 tggcgatggc ctgcttctcc tccacccgct cctcgcgggt cgcagagaag tgggattctt
      901 ccgccttccg aacctccaag accgctcccc cgtgaacctt gcgcaccagc ccgcggtccc
      961 gcaacagcga gaggtcacgg cgaaccgtca tctccgaagc ccggaccgct cgcgccagcc
     1021 tgcccacggg caccacacgg tggccctcca ggagggcgag gatcttctgg tgacggatca
     1081 acgggtgcac ggtcaaatcc tcaagcgaaa catctcacca cgaagactac aaggcaccac
     1141 gagtcatcaa gtctcaacgg atcagccaca gatcctgatc tcaatgttct accacttcgc
     1201 cgggatcgat cgcctctcga ggatgtccaa ggaacttctt ggtgccttgg tgtctttgcg
     1261 attgagctgt gcttcgatgc acgggtggat ggtgccgacc tgggatccct cccggcaggg
     1321 ccagaaccgc gaggatttgg tcgtggatct tcccattgct ggccacggtc tcgccatcgt
     1381 agatgtcgaa gccgccgccc cggaagtcgg acatgcggcc gcccgcctcc gtcacgatca
     1441 agcctcccgc cgcggtgtcc cagggggact ggtcgatctc ccagtagccg tcgaaccggc
     1501 cgcaggccac gtagcacaga tccagggcgc cggccccccg gtcgcggacc ccgagagttc
     1561 gcgcggagaa gcgaccaaag gtgcgggaca ccgatcccac gaatcgccgc accctgtgcg
     1621 gaagcccggt cgccaggaag gccgccctca aggtcgcaac gtcggaaacc cggatgggcc
     1681 tctcgttaca ccagaccccg tgcttgtgga cgccgacaaa ggtttcccca tgaatcggat
     1741 cgtggaccac ccccagctcc acccgaccct cgcgctcggc ggcgatagat acgcagaagc
     1801 tcgggacgcc ccgggcgaaa ttcttggtgc cgtccagtgg gtccaggatc cagcgccact
     1861 ccgcaccggg agcaaggttc aaaccctctt cggctagaac accgtgggcg gggaagcggc
     1921 ggcgcaaccg gccgacgatg agagtctgac actccagatc ggtggcggtc acgacatccg
     1981 cctgcccctt catccacacc tgcatggctc cccggaaccg gcgcttcagg atgcggccgg
     2041 catcctgggc cgagctgatc gcctcggcca ccaatggttg gagagttctt gggagctggc
     2101 tgatacggaa gcgctctgtg gtcatcaact ctcctctatg gggaaaagca tgacgtccgc
     2161 cgggtgcagg ctgaagcgga tctgctcccc ctccttgcgg aggcgggccg cgtccggctt
     2221 gtgctcgtcc agatgaatca gggagccgtc gccgagccgc aggcggtagc gcaggatggc
     2281 ccccaggaac tcccggctga taaccacccc gactcctggc gttgcctctg ggcacccctc
     2341 gccctgggtg agcacgccca cggcctctgg acggaacacc accagagccc ggtcggccag
     2401 gtcatccgat cgccgcgcgc gaaggctcca gccgccgacg tccacatgcg tccactcccc
     2461 ctgatggcct accacccggc ccgtgagggc attgctggtc ccgatgaact gggccacgaa
     2521 ggcgctctgg ggtgtctggt acacgtccca cgggaatccg acttgctcca cgtgtcccgc
     2581 gttcattacc gccagccgct ccgagatggc cagcgcttcc tcctggtcgt gggtcacgta
     2641 gatcgtggtg attcccagtt cgcgctgcag gcgtcggatc tccgtccgca tctccactcg
     2701 cagcttggcg tcgaggtttg aaagcggctc gtccatcaag agcaggcggg gctggatggc
     2761 catggcccgc gccagcgcca cccgctgctg ctggccaccc gagagctggt ccggccggcg
     2821 gtctgccagc gcctccatct gcaccaggtg gagcgcctcc gccgcgcggc gacccatttc
     2881 ggtcggcggc acccgccgcg cccggaggcc gtatgcgaca ttctcgaaca ccgtgagatg
     2941 cgggaagatg gcgtagtttt gaaagaccat gccgatgtcg cgcttatggg cc
//
LOCUS       DBNGZC010000290         2281 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_512488, whole genome shotgun sequence.
ACCESSION   DBNGZC010000290 DBNGZC010000000
VERSION     DBNGZC010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_512488"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2281
                     /locus_tag="AC16G0_05340"
     CDS             <1..>2281
                     /locus_tag="AC16G0_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HIR5099081.1"
                     /translation="GKTFAMLQAAHERRAEGVDLAAGWVETHGRAETEALLEGLEILP
                     PRRVEYRGTVLNEFDLDGALARRPTFLLVDELAHTNAPGVRHPKRWQDVLELLDAGIN
                     VYTTLNIQHIESLTDVIAKITRVVVRETVPDSVLDEADEVSLVDLPPDELLQRLKEGK
                     VYIPENAKEAIQNFFRKGNLIALRELALRRTADRVDIHMRSYMRDHAIGTTWPVAERL
                     LVCISSSPQSPQVVRAAKRMATALRAEWIVAYVETPGLVRLSEADRTRLVGTLRLAEQ
                     LGAETATLSGQRMSEEILAFARARNVTKIVIGKPKRPLWRRLLLGSIADGLIRGSGEI
                     HIHVISGEQGPAGAPLPRVRSKRTDWPDYGLAGIVVGLCTALAWGMFPYFDLSNLIMG
                     YLLGVVVVATRTGLGPSILASVLSVSAFDFFFVPPYYTFAVSDTQYFVTFGVMLVVAV
                     VLSNLAARMRVQAESARLRERRTAALYAMSRELASTRGGNEVLRAAVRHIAEVFRCQA
                     VVLLPDEVGELMRQIGLREQFDRDTTDLGASQWVYEHGQMAGPGTGTLPGATALYLPL
                     AASRGTIGVLGIRPNESHSLEAPEQLHLLEAFANQAALAYERAQLAEEAQQAQVRAEA
                     EQLRSSLLSSVSHDLRTPLASITGTISGLLEDDAQINRRTRRELLQSVYDEVERLNRL
                     VNNLLDMMRLESGAVVVKKEWYLLEEVVGTALAHMGKRLQGHSVSTRLPAELSLVQLD
                     AVLIDQVLVNLLDNALKYTP"
BASE COUNT          383 a          751 c          762 g          385 t
ORIGIN      
        1 gggaagacct ttgccatgct ccaggcagcc cacgagcgac gggccgaggg cgtggacctc
       61 gccgcgggtt gggtggagac gcacggacgg gccgagacgg aagcccttct ggaggggttg
      121 gagatcctgc cacctcgccg cgtggagtac cgagggaccg tcctgaacga gttcgaccta
      181 gacggcgccc ttgcccggcg gcccaccttt ctgctcgtgg acgagctggc tcacaccaac
      241 gcccccggtg tccggcaccc caaacgctgg caggatgtgc tggagctgct ggacgccggg
      301 atcaacgtct ataccacgct gaacatccag cacatcgaaa gcctcaccga cgtgatcgcc
      361 aagatcaccc gcgtggtggt ccgcgagacg gttcccgact ccgtcctcga cgaggcggac
      421 gaggtctcgc tggttgacct cccgcccgac gagctcctgc agcgactcaa ggaggggaag
      481 gtctatatcc cggagaacgc caaggaggcg atccagaact tcttccgcaa ggggaacctg
      541 attgcgctgc gcgagctagc cctccgccgc accgccgacc gcgtggatat ccacatgcgc
      601 agttacatgc gtgatcacgc catcggcacg acgtggcccg tggccgagcg cctcctcgtc
      661 tgcatcagct cgagcccgca gtcgccgcag gtcgtccggg ccgccaagcg gatggccacc
      721 gccttgcgcg ccgagtggat cgtggcgtat gtggagacgc cgggattggt ccgcctgtcc
      781 gaagccgacc ggacccggct ggtcggcacg ctccgcctgg ccgagcaatt gggcgccgag
      841 accgccacgc tcagcggtca gcggatgagc gaggagatcc tggccttcgc ccgtgcgcgg
      901 aatgtgacca agatcgtgat cgggaagccg aagcgcccgc tctggcgccg cctcctgctc
      961 ggctcgatcg ccgacggcct catccgcggc agcggggaga tccacatcca tgtcattagc
     1021 ggggaacaag gccccgcagg agctccgctc ccccgcgtcc ggtcgaagcg caccgattgg
     1081 ccggactacg ggttggccgg catcgtggtc ggactctgca ccgccctcgc atgggggatg
     1141 ttcccgtact tcgatctttc gaacctcatc atggggtatc tcctcggggt cgttgtggtc
     1201 gcgacgcgca ccggcctggg gccatccatc ctggcctcag tcctgagcgt cagcgcattc
     1261 gatttcttct tcgttccccc gtactacacg tttgccgttt cggacacgca atacttcgtc
     1321 accttcggcg tcatgctggt ggtggcggta gtcctcagca acctggcggc gcggatgcgg
     1381 gtccaggccg agtcggctcg tctccgggag cgacgcacgg cggccctgta tgccatgagc
     1441 cgagagttag ccagcacccg cggggggaac gaggtcctgc gggcggccgt gcggcacatc
     1501 gcagaggtct ttcgatgcca ggcggtagtg ctgcttcccg atgaggtcgg tgagctgatg
     1561 cgccagatcg ggctgcgaga gcagtttgac cgggacacca ccgacctcgg tgccagccag
     1621 tgggtgtacg agcacggcca gatggcggga ccgggaacgg gaacgctgcc gggggccacg
     1681 gcgctctatc tgccactggc cgcgtctcgt gggaccatcg gcgtcctcgg gatccggccg
     1741 aatgaatccc actctttgga ggcgcccgaa cagctccatc tcctggaggc tttcgcgaac
     1801 caggcggccc tcgcctacga gcgcgcgcag cttgccgaag aggcgcagca ggcccaggta
     1861 cgggcggagg cggaacaact gcgcagctcg ctgctcagct ccgtatccca cgatctccgt
     1921 accccccttg cttccatcac gggaacgatc agcggcctgc tggaagacga cgcgcagatc
     1981 aaccggcgaa cgcggcgaga gctgttgcag tccgtctatg acgaggtgga gcggctgaat
     2041 cgcttagtga ataacctgct tgacatgatg cgcctggagt ccggggcggt agtcgtcaag
     2101 aaggagtggt acctgctgga agaggtcgtc gggacggcgc ttgcccacat ggggaagcgg
     2161 ctgcagggcc attcggtgag cacgcgcttg ccggccgagc tgtccttggt gcagctcgac
     2221 gcggtgctga tcgatcaagt cctcgtgaat ctgctcgaca atgccctcaa gtacacgccg
     2281 g
//
LOCUS       DBNGZC010000291         4672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1491350, whole genome shotgun sequence.
ACCESSION   DBNGZC010000291 DBNGZC010000000
VERSION     DBNGZC010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1491350"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="AC16G0_05345"
     CDS             <1..313
                     /locus_tag="AC16G0_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099082.1"
                     /translation="LWERHLGDTEERVFRSIVAASPTADGLVGFFLAEHADLSARLTA
                     LAGSPPSAGRSQATIQLIHRLIQHVFLEARVLSPADRSGPRADACARIGAAKAHVLHD
                     T"
     gene            335..622
                     /locus_tag="AC16G0_05350"
     CDS             335..622
                     /locus_tag="AC16G0_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5099083.1"
                     /translation="MAKLNALRTREDVLELARAATKSYVSTSVTIEINDAWCKGCGIC
                     VEFCPKDVLVMNRTDKVEVQALQACTKCLRCEQLCPDFAIAVYGEPGKSSN"
     gene            693..1841
                     /locus_tag="AC16G0_05355"
     CDS             693..1841
                     /locus_tag="AC16G0_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR5099084.1"
                     /translation="MSPQVKPRVVKLLQGNEACAEAALTAGCRFFAGYPISPSSEIAE
                     HLSVRLPQVGGKFIQMEDEIAAMGAILGAAMTGAKVMTATSGPGYSLKQENIGYAVMA
                     EIPCVIVNVMRGGPSTGLPTSPAQADVMQARWGTHGDHPAVALVPASVREVYDETIRA
                     FNIAERFRIPVSLLIDEIIAHMRERVELPDPSEIIRWERPRPTVPPDQYLPYGDTPTG
                     VPPMADFGKGYRYHVTGLYHDVRGLPKDSPEVVDAMMRRLMRKVELGTPDILKHEEML
                     LEDANLVVVAYGSSARSAKAAIRLARGQGIRVGLLRPLTIWPFCEDAVAALSRRVRHL
                     LVVEMNMGQLALEVERASHGACPVHRVNRVTGEPIPPEQILEKILELA"
     gene            1905..2696
                     /locus_tag="AC16G0_05360"
     CDS             1905..2696
                     /locus_tag="AC16G0_05360"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HIR5099085.1"
                     /translation="MFPHIWCPGCGDGIVMKSLLRAIDRVGLEKDDICMVSGIGCSSR
                     LTGYVDFNTLHTTHGRPLAFATGVKLAKPSLNVIVVTGDGDALAIGGNHFIHAARRNL
                     DLTVLLFNNSTYGMTGGQLAPTTPTGKVASTARFGSVDPPFDACKLAIAAGATYVARG
                     TSYHTQQLDRLIEGGIKHKGFALIEILENCNTYYGRPNKIGLLDLLQWEKNNTVNVKA
                     AERMTPEQLQGKIVTGLLHQSDRPEYVALYDQLIGQAQAKLAAAS"
     gene            2730..3509
                     /locus_tag="AC16G0_05365"
     CDS             2730..3509
                     /locus_tag="AC16G0_05365"
                     /inference="COORDINATES: protein motif:HMM:NF013707.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR5099086.1"
                     /translation="MGYHYEIRLSGEGGQGIVLAGVILAEAAAIYDGKNATQTQVYGP
                     ESRGGASKAEVIISDEEIDYPKAISVDLLLAMTQAAADKFSHDLKPSGILVLDSSKVQ
                     NVPQGDFTVHHLPIIETAKETVGKAMVANIVSLGVIVGLSKVVSRHAIEEAVLARVPK
                     GTDELNRRALEAGFSRAGAGYNTRLSASQRHASSPARDDSFVRASFFRPLPFPFLGNF
                     PSTFVRQSVSTPYRGGTGEDEREEESYLREAETQVRQRPSR"
     gene            3669..4055
                     /locus_tag="AC16G0_05370"
     CDS             3669..4055
                     /locus_tag="AC16G0_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5099087.1"
                     /translation="MTSPHLLIVEDDSEMRDLLRKVLEKDGYQVSVATDGHDAIPQLS
                     RGHFDLVVTDMLMPHDGGLELLEFIRKTQPMLPVIIITAFGDWQSYSRALELGAVAFI
                     SKPLKMAELVSAIQTALAGSGAGQAA"
     gene            4087..4551
                     /locus_tag="AC16G0_05375"
     CDS             4087..4551
                     /locus_tag="AC16G0_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5099088.1"
                     /translation="MLRIRRILFPTDFSEYAEHAWSYGLTFAKEFGAEVHLLHIVTPP
                     PRLTEAYAVNFDPEGLVQALRAEATAALEKLAQAAKDHGLACHTEIRVGVDFREIITY
                     ATTQGIDLIVMATHGRTGLAHALLGSVTEKVVRKAPCPVLTVKHPSMKVEMP"
BASE COUNT          869 a         1570 c         1444 g          789 t
ORIGIN      
        1 cctatgggag cggcacctcg gcgacaccga ggaacgggtg ttccggtcga ttgtcgcggc
       61 aagccccacg gctgacggcc tcgtgggatt tttcttggcg gagcacgccg acctttccgc
      121 ccggctcaca gcgctggcgg ggtccccacc ctcggccgga cggtcacagg ccacgatcca
      181 gctcatccac cgtttgatcc aacatgtctt cctggaggct cgagtcctgt cccctgcaga
      241 ccgatcgggc ccgagggcgg acgcctgcgc tcgcatcgga gctgccaagg cccacgtcct
      301 tcacgacacc taggggagag aggttcacaa cgtcatggcc aagctcaatg cgctccgtac
      361 tcgtgaggat gttctggagt tagcccgagc agccacgaag agctacgtca gcaccagcgt
      421 gacgattgag atcaatgacg cctggtgcaa gggctgcggg atctgcgtcg agttctgtcc
      481 gaaagatgtc ctggtgatga accgcacgga caaagtggag gtccaggccc tgcaggcttg
      541 caccaagtgc ctgcggtgcg agcaactatg ccccgacttc gccatcgctg tttacgggga
      601 gcccggaaag tccagcaatt agaccgacag gcgcgctccc gcgcccgttg ggtcggcagg
      661 cgccggaagc cgggtctctc gacggaggcc gcgtgagtcc acaagtgaaa ccccgcgtag
      721 tcaaactact ccagggcaac gaggcctgcg ccgaggccgc cttgaccgcc ggttgccgct
      781 tcttcgccgg ctaccccatc tccccctcgt ccgagatcgc cgagcacctg tcggttcgcc
      841 tgccccaggt cggcggaaaa ttcatccaga tggaggatga gatcgccgcc atgggggcaa
      901 tcctgggcgc cgccatgacc ggggccaagg tgatgaccgc gaccagcggg cctggctatt
      961 cgctgaagca ggaaaacatc ggatacgccg tcatggccga gatcccgtgc gtgatcgtca
     1021 acgtcatgcg ggggggaccg agcaccggct tgccgacatc tcctgcgcag gcggacgtga
     1081 tgcaggcgcg ctgggggacc cacggggatc acccggccgt ggccctcgtg ccggcctcgg
     1141 tgcgcgaggt ctacgacgag acgatccgcg ccttcaacat cgccgaacgt ttccgcatcc
     1201 cggtgagcct gctcatcgac gagatcatcg cccacatgcg cgagcgggtg gaactgccgg
     1261 acccgtccga aatcattcgc tgggagcgac cgcggcccac ggtccccccg gaccagtacc
     1321 tcccctacgg cgacaccccg accggcgtgc cccccatggc cgatttcggc aagggctacc
     1381 ggtatcacgt caccggcctt taccacgacg tacgcggcct tccgaaggac agcccggagg
     1441 tggtggacgc catgatgcgg aggctcatgc ggaaggtcga gttgggcacg ccagacatcc
     1501 tgaagcatga agagatgctc cttgaagatg ccaacctggt cgtcgtggcc tacggctctt
     1561 ccgctcgctc cgccaaggcg gccatccgct tggcacgggg ccagggaatc cgggtcgggc
     1621 tcctgcgccc cctgaccatc tggcccttct gcgaggatgc cgtggctgcg ctctcacggc
     1681 gggtgcggca ccttctcgtg gtcgagatga acatgggcca gctggctctg gaggtcgaac
     1741 gggcgagcca tggcgcctgc ccggtccacc gcgtgaatcg cgtgaccggc gagccgatcc
     1801 cgcccgaaca gatcctggaa aagatcctcg agttggccta gggccgacgc cgacacagga
     1861 ggaggacgcg cgtgttcagc tacgagaagt acctgcgcct ggagatgttc ccgcacatct
     1921 ggtgtccggg ctgcggggac gggatcgtga tgaagagcct gctccgcgcc atcgaccggg
     1981 tgggcctgga gaaggacgac atctgcatgg tgtcgggcat cggctgctcc agtcgcctca
     2041 ccgggtacgt ggacttcaat acgctgcaca ccacccacgg gcggccgctc gccttcgcca
     2101 ccggggtgaa gctggccaag ccctccctca acgtcatcgt ggtcaccggg gacggtgatg
     2161 ccctggcgat cggcgggaac cacttcatcc acgccgcgcg gcgcaacctc gacctgaccg
     2221 ttctcctctt caacaactcg acctacggca tgaccggcgg ccaactcgcc cccaccaccc
     2281 cgaccgggaa ggtggccagc accgcacgct tcggcagcgt ggaccctccc ttcgatgcct
     2341 gcaagctggc cattgccgcc ggcgccacct acgttgcccg ggggacctcg tatcacaccc
     2401 agcaactgga ccggctcatc gagggcggga tcaagcacaa aggcttcgcc ctgatcgaga
     2461 tcctggagaa ctgcaacacg tactatggcc gccccaacaa gatcggcctg ctggacctgc
     2521 tgcagtggga gaagaacaac accgtgaatg tcaaggcggc ggagcggatg acgccggagc
     2581 agctccaggg gaagatcgtc accggcctcc tccaccagtc cgatcggccg gaatacgtgg
     2641 ccctttacga tcagctcatc ggtcaggcgc aggccaagct tgcggcggcg agctgatcct
     2701 cattccccat ttccctcggg aggcgctgca tgggttacca ctacgagatc cgcttgagcg
     2761 gtgagggcgg acagggtatc gtcctggcgg gcgtgatcct ggccgaggcc gccgctatct
     2821 atgacggcaa gaacgccacc caaacccagg tgtacggacc cgaatcgcgg ggcggcgcca
     2881 gcaaggccga ggtcatcatc agcgacgaag aaatcgacta ccccaaggcc atctccgtgg
     2941 acctgctcct ggccatgacg caggcggccg ccgacaagtt cagccacgac ctgaagccgt
     3001 cggggatcct ggtgctggat tcctccaagg tccaaaacgt gccgcaaggg gacttcaccg
     3061 tccaccacct gccgatcatc gaaacggcca aggagaccgt gggcaaagcc atggtcgcga
     3121 atatcgtgtc cctgggggtg atcgtcggcc tgtccaaggt ggtctcccgc catgcgatcg
     3181 aggaggccgt cctggcccgc gtgcccaagg gcaccgacga gctgaatcgc cgcgccttgg
     3241 aggcaggatt ctcccgcgca ggagcagggt acaacacccg tctgtcggct tcccaacgtc
     3301 acgcttcctc tcccgcccga gacgattcct tcgtcagagc atccttcttc agacctcttc
     3361 ccttcccctt cctaggcaac ttcccctcta cattcgtgag acaatcagtc tcaacccctt
     3421 acaggggtgg gacaggggaa gatgagagag aggaggagtc gtatttgagg gaagcggaga
     3481 cccaggtccg ccagaggcca agtcgttgaa aacggttggc tcttaccgcc ctcggcctgg
     3541 tcaaagtctg gcacggcttc tcctcagggc accgaatccc atcttttcgt ggcattggct
     3601 ttgcaccgca agaccgtgtt atgactccta tctcccactc ccacccgaga tcgcttcaag
     3661 gtgccgaaat gacgagccca cacctgttga tcgtggaaga cgactcggag atgcgtgacc
     3721 tcctccgaaa agtcctggaa aaggacggct atcaggtttc ggtggcgacc gacggtcacg
     3781 acgccatccc gcagctctcc cggggccact tcgatctcgt ggtaaccgac atgctgatgc
     3841 cgcatgacgg tggactcgaa ctcctggaat tcatccgcaa gacccagccg atgctgcccg
     3901 tcatcatcat cacagccttt ggtgattggc agagctatag ccgggccctc gagctggggg
     3961 cggtggcgtt catcagcaag ccgctgaaga tggcagaact ggtctccgcc atccagacag
     4021 ccctggcggg gagcggcgcg ggtcaggccg cttgagggat cgcggcgtcg ccgcggggag
     4081 aacgccatgc tacgcatccg acggatcctg ttcccgaccg acttctcgga gtacgccgag
     4141 cacgcctggt cctacggcct gacatttgcc aaggagttcg gagctgaagt gcacctgctc
     4201 cacatcgtca cccctcctcc gcgtctgacc gaggcctacg cggtgaactt cgatccggag
     4261 ggcctcgtcc aagcgcttcg cgccgaggct accgccgccc tggagaaact cgcacaagca
     4321 gccaaggatc atggcctcgc ctgccacacc gagatccggg tgggcgtgga ctttcgtgaa
     4381 atcatcacct acgccacgac ccaagggatt gacctcatcg tgatggcgac ccacggccgc
     4441 accggcctgg cccatgcgct ccttggcagc gtgaccgaaa aggtcgtgcg caaggcaccc
     4501 tgtccggtgc tcaccgtcaa gcacccatcc atgaaggtgg agatgcctta gggcatcgct
     4561 agggggagcc cctgatgttc tcccgcatcc tggtccccct ggacggctcc gcgctggccg
     4621 aggctatcct gccgcaggtg accgagttgg ccacgctgca tggggcagag gt
//
LOCUS       DBNGZC010000292         5226 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1491391, whole genome shotgun sequence.
ACCESSION   DBNGZC010000292 DBNGZC010000000
VERSION     DBNGZC010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1491391"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(585..842)
                     /locus_tag="AC16G0_05380"
     CDS             complement(585..842)
                     /locus_tag="AC16G0_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099089.1"
                     /translation="MTPNEIVIATETARSAPGALVGIGIETDHSVPGALIATATGTGR
                     SAPPAGVVIGIGMHRSIPGALIATATGAGLLIVTRGSAASA"
     gene            complement(1008..1295)
                     /locus_tag="AC16G0_05385"
     CDS             complement(1008..1295)
                     /locus_tag="AC16G0_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099090.1"
                     /translation="MPKTAGVGVTAVVTGVTVAGATAVARAVITTVAEAAVAVTVVTA
                     IPVGEVGREVALAARAGAWADKPPVLGVAEVTMVGGRPRRPATAIIGQEPR"
     gene            complement(1883..2704)
                     /locus_tag="AC16G0_05390"
     CDS             complement(1883..2704)
                     /locus_tag="AC16G0_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HIR5099091.1"
                     /translation="MSILAISRGSFSRGEAVAKGVADRLGYRCVSREVLFEAAWGYGI
                     PAEELMATMERRPPLWARLAGKRTAHLICMRAALCEYARGGNLVYHGHLGHLLLPGIT
                     HVLRVRVIADLADRIQAAMGHRHLGRQEAMVYIEKMDKERRQWTRFLFDVDWNDPAQY
                     DLVLNLWRMRLSTACDTVVQLVAQPEFTPTPASVKALQDLALCSQVEAVLARDPRTMG
                     ANFEVTANDGSVTVTGTTQSAELIAAIPLVARQVDGVKELTNAVRLLQEGIVVPY"
     gene            complement(2891..3730)
                     /locus_tag="AC16G0_05395"
     CDS             complement(2891..3730)
                     /locus_tag="AC16G0_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099092.1"
                     /translation="MVDQNGVVEPIREAGAGGEARIIVELRTRFPGLGGTILTQEGVA
                     AALRIEYNQAPLVGHEMFIRHLIAKKLDDHFYRSGVYVFAHIPRVLGSISLGEREPRE
                     AYLYEWAFGSDGFSWLEPGTNDPVILHDWNPFVGCFAGAGVDLGYDTTDPDDGRVSQN
                     VVHQYPESLLSGEMCSLWKRIDFGFHSIRIDLDRLSAFVHDQKAKLMTTLRYERCEML
                     RLAVTYLRHRRYKEPISDHDLGRLEQLICEFRRSSLSHYAFAFGSDKGDVQLTGESQT
                     LLQ"
     gene            complement(4540..>5226)
                     /locus_tag="AC16G0_05400"
     CDS             complement(4540..>5226)
                     /locus_tag="AC16G0_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR5099093.1"
                     /translation="AIGGTSFGDIALVPAPGLKYPKGIRDITEWYISILTRQDYIHAI
                     FARQCELALDKLARVHAVIGDAIDVLYVCGTDFGTQTSSFCSTDTFDSLWAPYYRRIN
                     DWVHRHTTWKTMKHCCGAVEPFMPHFIAAGFDVINPVQCSAAGMDPRLLKTRYGDRLV
                     FWGGGVNTQQTLPFGTPEEVRAEVLERCEIFSRGGGFVFNAVHCVQANTPLPNILAML
                     DAVREFNGAR"
BASE COUNT          954 a         1665 c         1623 g          984 t
ORIGIN      
        1 ggataccttt tccgccagcg ggaggagatc cttcatcggt ggttcctttt cataggacct
       61 gaggtagtga ccatcttgat gggttatggg gggttgcatt attggtggag cggctccacg
      121 taggccagcg agccaacggc cattgagtct ctggcattgc ctggaatcga ctgggtcgcc
      181 gctgactccc acggcagccg gagggatcct cgaccaggtt gaagtcgagg accctccggt
      241 gatgctcggt cggcagggca atcgttggac taaaccgatg gagcctgggg gaccgtcttg
      301 tcaggtgggg cattgcccga gagtggggat cggcagggga taattcctgg aaagcagccg
      361 tcccactttg tgtcgtgtcg gatagctccc agccgtctcc gcctccctcg ggcacacccc
      421 ggccctggtc ttgccagcag agccacgaag attgcagcct ctccgtgggg gggtcctccg
      481 aagctcttgg agctaatccc cgctgagtcc gactcctccc ggcgctacca gtgtgcccat
      541 cccccgtgta cggaactgcg ggggattcgt ggccgaggaa acctctacgc cgaggccgcg
      601 ctgcctctgg tgacgatcag caaaccggct ccggtcgcgg tcgcgatcaa agcgcccgga
      661 atggaacgat gcatcccgat cccgatcacg accccggcgg gcggagcgga acgacccgtc
      721 ccggtcgcgg tcgcgatcaa agcgcccgga acggaatgat ccgtctcgat cccgatcccg
      781 actaaagcgc ccggagcgga acgagccgtc tcggtcgcga tcacgatctc gttgggcgtc
      841 atggactgcc gtgcgcacgg cctggccaag attcgagaat ccggctgctc gcgccgctgc
      901 tctgcctgcg tgtgcggttg tcgtggcggc ttcggagacc tcattcccga cctcgcggcc
      961 cggggcgcga tgtcctcggc cgaaggtggt gtgctcgctt gtcgctgtca tcgaggctcc
     1021 tgaccgatta tggccgtggc tggccgccgc ggcctccccc cgaccatcgt gacttctgcc
     1081 acgcccagca ctggaggctt gtccgcccac gcccccgctc tggccgccaa agccacctcc
     1141 cgtccgacct cccccaccgg aatcgctgtg accaccgtta ccgccacggc cgcttcggcc
     1201 accgtcgtga tcaccgcccg cgctactgcc gtggctcccg ccaccgtcac ccccgtgacc
     1261 accgccgtga cccccacccc cgccgtcttt ggcataggcg acgggcgaat cgagcactgc
     1321 atgcggcacg tgcagttcgg ccaggaacgg cacaaccagc acagagagga tcgccaccag
     1381 caggacccgc gagattctcc ccattgtgct tccctcctcg gagaatgaac ccatcatcca
     1441 gatccaggac cgtactggtc ctctatcctt ataatatgcc taggatgggg aagtccatac
     1501 ttttctggga acctactgct ctccagggat gatcctgcac ttcctcccat aatctctcgc
     1561 gcagaaacac tgcaccattt ccctgaggcc tcgtgaacct cggtcgcacc ccccttccac
     1621 tcaccgcaag caccgattca gcctgaggca ccccggtact tcttgtggat atactggaga
     1681 aaaaatgggt ggccactacg gcaagcgtct tggtgtctca agggtgaaga cgggagcagg
     1741 cgatgcgaat cgcactcact gagcaggttg atgtgctgcc gcgtcactca ggagccgtgg
     1801 gccgcgactg cacgaaacac aggcgagcga gtgaccggaa aggcctcggg ccggaacgat
     1861 tacccgtgcc gtatgaggcg cgtcaatagg ggaccactat cccctcctgg agaagccgaa
     1921 ccgcattcgt gagttccttc accccatcga cctgacgggc aaccagcggg atggccgcga
     1981 tcagttccgc cgactgggtc gtgcccgtga cggtgacgct gccatcattg gcggtgacct
     2041 caaaatttgc ccccatggtg cgggggtctc gggccaagac ggcctccacc tgactgcaga
     2101 gcgcgagatc ctgcagggcc ttcaccgagg cgggggtcgg tgtaaactcc ggttgtgcga
     2161 ctaactggac cacggtgtcg catgcggtgg ataggcgcat ccgccacaag ttcaagacga
     2221 ggtcgtactg ggcggggtcg ttccagtcca cgtcgaacag gaatcgggtc cactgtcgcc
     2281 gctccttgtc catcttctca atgtagacca tcgcctcctg gcgcccgagg tgccggtgcc
     2341 ccatggccgc ctgaatgcgg tcggcgaggt cggcgattac ccgaacgcgc agcacatggg
     2401 tgatgcccgg caacaggaga tgtccgaggt ggccgtgata gacaagattg cccccacggg
     2461 catactcgca cagtgccgcc cgcatgcaaa tgaggtgggc ggtccgcttg ccggccaggc
     2521 gagcccatag ggggggccgc ctctccatgg tggccatgag ttcctccgcc ggtatcccat
     2581 acccccaggc tgcctcgaag agtacttccc ggctcacgca ccgataaccc agccgatcag
     2641 ctacaccttt ggccactgcc tccccccggc tgaagctccc acgcgaaatg gctaggatgg
     2701 acatgggtcc tccctttctg cccaatcagg accttgaggt tccaccatct tggcctggtt
     2761 caaccccgga tctgcgagtt gtgccatctt cgcccactct cccaagcgct gctttcagcc
     2821 cccatggtcg cacaggctcg cgaggaagta caaggtagct cacggcactg atccgacatc
     2881 gagcggagcg ttactgcaat agcgtctggg attccccggt cagctggaca tcgcctttgt
     2941 cggacccgaa ggcgaaggcg tagtgactga ggctggaccg gcgaaattcg cagatcaact
     3001 gttcaagccg cccgagatcg tggtcgctga taggttcctt gtatcttcgg tggcgcagat
     3061 atgtaacggc caggcgaagc atctcgcagc gctcataccg cagggtcgtc atcagcttgg
     3121 ctttctgatc gtggacgaag gccgacagac gatctaggtc aatgcggatg ctgtgaaagc
     3181 cgaagtcaat ccgcttccac agtgaacaca tttctcccga cagcagactt tccggatact
     3241 gatgcacgac gttttgtgag acccgcccgt cgtccgggtc tgtggtgtcg tagccaaggt
     3301 cgacgccggc acccgcaaag cacccgacga atgggttcca atcgtggagg atgacggggt
     3361 cgttcgtacc gggctccagc caggagaacc cgtcgctgcc gaaggcccac tcataaaggt
     3421 acgcctcacg gggctctcgc tcgccaagac tgattgatcc caagacccgg ggaatgtgcg
     3481 caaacacata gactcccgat cggtaaaagt gatcatccag cttcttggcg atgaggtggc
     3541 ggatgaacat ctcgtggccg accagcgggg cctggttgta ctcgatccgg agggcagcgg
     3601 cgacaccttc ctgggtcagg atagtccccc ccagtcctgg aaatcgtgtc cggagttcca
     3661 cgatgatacg cgcctcgccg cctgcgcccg cctcgcggat gggttcgacg accccgtttt
     3721 ggtcaaccat gtgaggatct cctctggagt cagccagtct ggcgtacctt ccgccgcaac
     3781 gcgccatcca cgatccccat tcggggccga cacaccttga ttggatacgg ccccctggca
     3841 gtcaggcttc ggatgaaatg caccacccga gcttggccct ccatgtgtgc gaggagcacc
     3901 cgctcgagga ggttctaggg agtacgagga gtactttggt cttctgctcg aagatcgcat
     3961 taccttttat tcctgccggc cggtgagcct caccaccgac ccggggtgtt caggatgtcc
     4021 ttctgacttc ctatgtaaaa gctatacctt gccccacgtc acatcaaggg cagatgccag
     4081 gactgcttgg ctcggtaaaa tcccacggat cattgcaggt ttctcacggg gcactactgc
     4141 acccgcaaac cgctgtattc tcgattgttg gcttccactc aaggtcggcg acgacgtggt
     4201 cgggagagcg gatgtgtccg ggccgtcggg aagggacgat gagtgtctca aggcgggctt
     4261 cgactaggcg gccgatctgc ttactgtgcg gtgcttagaa taggcgacgc ttcctccacg
     4321 gccgcacagt ccgggggaga gtccgaggag gggcggcggc ccgcccctct cggtagggtc
     4381 cgcggggagg gcagcgccct tgcgttgcac cctgtgcaag ggcgagggcc ttccccgcga
     4441 acggagcagc gcggggaacg gtgtagccag tattccgcgc tgcgtagtaa gtgggccggc
     4501 gggctcatcc tccgccccaa gggtgagatc gcgcgcgagt cagcgggccc cgttgaactc
     4561 cctcacggca tccagcatgg cgaggatgtt cggcagcggc gtattcgcct ggacgcagtg
     4621 gacggcgttg aagacaaagc cgccgccccg ggagaagatc tcgcagcgct ccaggacctc
     4681 cgcccgcacc tcttcgggtg tgccaaaggg aagggtctgc tgggtgttca cgcctccgcc
     4741 ccagaagacc aggcggtcac cgtaacgggt cttgagcagg cgcgggtcca tgccggcggc
     4801 ggagcactgc acggggttga tgacatcgaa gccggcggcg atgaagtgag gcatgaacgg
     4861 ctcgacggcc ccgcagcaat gcttcatggt cttccaggtg gtgtggcggt ggacccagtc
     4921 attgatccga cggtagtagg gggcccagag gctgtcgaag gtgtcggtgg agcagaagct
     4981 ggacgtctgc gtgccgaagt cggtgccgca gacatatagg acgtcgattg cgtcgccgat
     5041 gacggcgtgg acgcgggcga gcttgtcgag agccagttcg cactggcgag caaagattgc
     5101 gtggatgtag tcctgccggg tgaggatcga gatataccac tcggtgatgt cccgaattcc
     5161 cttgggatac ttgaggcccg gcgccgggac cagggcgata tctccgaagc tggtcccacc
     5221 tatggc
//
LOCUS       DBNGZC010000293         5653 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_610545, whole genome shotgun sequence.
ACCESSION   DBNGZC010000293 DBNGZC010000000
VERSION     DBNGZC010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_610545"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /gene="malQ"
                     /locus_tag="AC16G0_05405"
     CDS             complement(<1..1202)
                     /gene="malQ"
                     /locus_tag="AC16G0_05405"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIR5099094.1"
                     /translation="MSDDGRGIHLPPFPREYRASGLLLHITSLPSPYGIGDVGPAAVA
                     WVDRLHEAGQTWWQALPLGPTGYGNSPYQPLSSFAGNGLLISPDWLIEDGLLRASDVA
                     GHSFPGTAVEYDAVMPFKHRLLETAWTNFNGGARPDLAPPFEQFCQDHAHWLEDFALF
                     RVLKERYHGAYYLDWPAELVQRTPAALDRARRELGSQIAQVCFAQFLLFRQGARLKEH
                     GQRKGVRLIGDLPFFVSPDSSDVWANPELFLLDEQCRPRVVAGVPPDYFSAQGQLWGN
                     PIYDWDALRRTGYRWCIDRLRALLAHVDVIRLDHFRAFVDAWHIPAGAPTAQTGHWVA
                     GPGGEFFTAVQAELGGLPFIAEDLGLITPDVYTLRDQFRLPGMRVLQFAFDGHSDNPY
                     LPHNYVSNA"
     gene            complement(1195..2880)
                     /gene="pgm"
                     /locus_tag="AC16G0_05410"
     CDS             complement(1195..2880)
                     /gene="pgm"
                     /locus_tag="AC16G0_05410"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HIR5099095.1"
                     /translation="MGISPLAGKPAPKEMLVDVGRLEREYYDRRPDLADPNQRVSFGT
                     SGHRGSPRRGAFTEAHILAITQAICDYRRAQGIDGPLYMGKDTHALSGPAQRTAIEVL
                     AGNRVETIIQRDDGVTPTPVISRAILVYNRGRRDNPADGIVITPSHNPPEDGGFKYNP
                     PNGGPADTDVTGWVQHRANKLLKGGNAGVKRVLFASAIKAGTTHHEDFILPYVNDLKN
                     VIDMDVIRGAGLKLGVDPLGGAAVHYWEPINAIYKLDITVVNPKIDPTFFFMTVDHDG
                     EIRMDCSSPYAMARLVGLKDQYRVAFANDPDSDRHGIVTPSAGLMNPNHYLAVAIRYL
                     LTHRPHWPEHAAVGKTLVSSSMIDRVVQKLGRRLCEVPVGFKWFVPGLFDGSVCFGGE
                     ESAGASFLRRDGTVWTTDKDGPILDLLAAEITARTEKDPGEHYRELTAEFDSPCYTRI
                     DAPATPEEKASLAKLSPEAVTASSLAGEPITAKLTRAPGNNASIGGLKVVAASGWFAA
                     RPSGTEDIYKIYAESFRDQTHLDAIVTEAQQIVHNALRSSEPVRGAEMDVRHE"
     gene            complement(2924..5425)
                     /locus_tag="AC16G0_05415"
     CDS             complement(2924..5425)
                     /locus_tag="AC16G0_05415"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HIR5099096.1"
                     /translation="MKTAAPQAATASEAVAALLRQYGCGPVELTGTPDALYERHLLFD
                     NVVNPTAAGPRERFEAIARSVRDVLSQRWVLTEDTYVRENPKRVYYLSMEFLIGRALA
                     NNVMNLLLDPVVKDAAAQMNLDWLGLLEEEPDAGLGNGGLGRLAACFLDSMATMQLPA
                     MGYGLRYEYGIFKQAIRDGWQQERPDNWLRRPDPWEVARPDESVEVNLNSSFELRGGV
                     LHPVPGRPTTLIGIPFDRPVVGYGGKTINTLRLWAAAAPDYFDFQAFSHGDFVGALAE
                     TLAAESLTRVLYPDDSTSMGQGLRFVQEYFLVACSLADLVRRFRRSNADWGTLPEKVA
                     IQLNDTHPALTVPELMRILLDDAKLGWDQAWDLTRRALAYTNHTLLPEALERWPLALF
                     EDLLPRHLEIIYEINRRLLETVRIRFPGDAGRVARVSLMEEGAAKHIRMANLAIVGSH
                     STNGVAAIHSELLRTTTVKDLAELFPERFNNKTNGVTPRRWLLLANPALAQTITAAIG
                     DGWITDLEQLRKLRSLADDRGFRDAFRKAKRAAKVQFAAWLKATSGRTVDPDRIFDCQ
                     VKRIHEYKRQLLNALRIVVLYHRLRENPNLEMTPRTFFFAGKAAPAYHLAKLVIKFIN
                     SLASTIEGDPATRNRLQVLFLPEYNVSLAEQLIPASDVSNQISTAGYEASGTSNMKFM
                     MNGALTIGTRDGATIEMAQEAGERNFFLFGLTADQVAASRGWYNPRWHYDNEPETRAS
                     LDLIFSDHFSRHEPGVFAPLGDTLLTHGDHYMHLADLAAYLEADRRLLQLYADPEEWA
                     RRAIVNVASSGKFSSDRTIAEYAANIWNAKPCPVP"
BASE COUNT          943 a         1838 c         1849 g         1023 t
ORIGIN      
        1 gcgttggaga cgtagttgtg cggcaggtat gggttgtccg agtggccgtc gaaggcgaac
       61 tgcagcaccc gcatccccgg caggcgaaac tggtcccgaa gggtgtacac gtcaggcgtg
      121 atcagcccca agtcctcggc gatgaaggga agcccgccca gctccgcttg gaccgccgtg
      181 aagaactcgc cgcccggccc cgctacccag tgaccggtct gggccgtcgg ggcgccggcc
      241 gggatgtgcc atgcgtccac gaacgcgcga aaatgatcta ggcgaatcac atcaacatgg
      301 gccagcaggg cgcgcaagcg gtcaatgcac caccgatagc cggtccggcg gagggcgtcc
      361 cagtcataga tcgggttgcc ccagagctgg ccctgggcac tgaagtagtc gggcggcacg
      421 ccggcgacga cacgcggtct gcattgctcg tccagcagga acagctcggg gttggcccac
      481 acgtcgctcg agtcggggga aacgaagaag ggcagatcgc caatcaaacg cacgcccttc
      541 ctctggccat gttccttcag gcgtgcccct tggcggaaca ggaggaactg ggcaaagcaa
      601 acttgcgcga tctgactccc cagctctcgt cgagcccggt ccagggcggc cggagtccgc
      661 tggaccaact ctgccggcca gtcgaggtaa taggcgccgt ggtaccgctc cttcagcact
      721 cggaacaggg cgaaatcttc cagccagtgt gcgtggtcct gacagaactg ctcgaagggc
      781 ggcgccaggt ccggtcgggc ccccccattg aagttggtcc aggctgtttc gagcagccga
      841 tgcttgaagg gcataaccgc gtcgtactcg acggcagttc ctggaaacga gtggccggca
      901 acgtcgctcg cccggagcaa gccatcctcg atcagccagt ccgggctgat caggagcccg
      961 ttgccggcaa acgacgagag gggctgatac ggcgagttcc cgtacccggt gggccccagt
     1021 ggcagcgcct gccaccacgt ttgcccggcc tcgtggagcc ggtccaccca tgccactgcc
     1081 gccggcccca catcgccaat cccgtagggg gagggcagcg atgtgatgtg gagcagcagg
     1141 ccggaggccc ggtactcccg cgggaacggt ggaagatgta tgccacgacc gtcatcactc
     1201 atgtcgtacg tccatttcag ccccccggac cggctcggag gatcgcagcg cgttatgcac
     1261 aatctgctga gcctcggtca cgatggcatc taggtgagtc tgatcccgga agctttccgc
     1321 atagatcttg tagatgtcct ccgtcccgga cggccgggcc gcgaaccagc cgctggcggc
     1381 caccaccttg aggccgccga tggaggcgtt gttgccgggg gcacgggtca gcttggccgt
     1441 gatcggctcg cccgccaggc tcgacgccgt caccgcctcg ggcgacagtt ttgccaggct
     1501 cgccttctcc tctggcgtcg ccggcgcgtc gatacgcgtg taacacggcg agtcgaactc
     1561 cgccgtcaat tcacgatagt gctcgcccgg gtccttctct gtccgggccg tgatctcggc
     1621 cgccagcagg tccagaattg ggccgtcctt gtcggtcgtc cacacggtgc cgtcacgccg
     1681 gaggaagctc gcccccgcgc tctcctcgcc cccaaaacag accgagccgt cgaacagacc
     1741 gggcacgaac cacttgaagc cgacgggcac ctcgcagagc cgacggccga gcttctggac
     1801 cacccggtcg atcatgctgc tgctgaccag ggtcttgccc acggcggcat gctcgggcca
     1861 gtgagggcgg tgcgtgagca gataccggat ggccaccgcc aggtagtggt tggggttcat
     1921 caagcccgca gagggggtga caatcccgtg ccggtccgag tccggatcgt tggcaaaggc
     1981 cacccggtac tgatccttca ggccgacgag tcgggccatc gcgtacgggc tggagcagtc
     2041 catgcggatc tcaccgtcgt ggtcaacggt catgaagaag aatgtcggat caatcttcgg
     2101 attcaccacg gtgatgtcca acttgtagat cgcgttgatc ggctcccagt aatgcacggc
     2161 agcgccgccg agcggatcca cgccgagctt caggccggcg ccccggatga cgtccatgtc
     2221 gatgacgttt ttcaggtcat tgacgtaggg taggatgaag tcttcgtggt gcgtggtccc
     2281 cgccttgata gccgatgcga acagcacgcg cttcacgccg gcattgcccc ctttcagcag
     2341 cttgttggcg cggtgttgca cccaacccgt cacgtcggtg tcggccgggc ccccgttggg
     2401 cgggttgtac ttgaagccgc cgtcctccgg cgggttgtgc gagggcgtga taacgatccc
     2461 atcggcgggg ttatccctgc ggccgcggtt gtagacgagg atggcccgcg agatgacggg
     2521 cgttggcgtg acgccgtcgt cgcgctggat gatcgtctcg accctgtttc ccgctagcac
     2581 ctcgatggcg gtgcgctggg ctggcccgga cagggcgtgg gtatcctttc ccatgtagag
     2641 cgggccatcg atcccctggg cgcggcggta gtcgcaaatg gcctgggtaa tggccaggat
     2701 atgggcctcc gtaaacgcgc cgcgacgcgg tgagccccgg tgcccgctgg tgccaaagct
     2761 gactcgctgg ttcgggtctg ccaggtcggg cctgcggtca taatactccc gttccagccg
     2821 gcccacgtcc accagcatct ccttcggagc tggcttgcct gccagcggcg agattcccat
     2881 gtctcttagt cctttctttg gtctttcctc aagccatgct cccctacggc accggacatg
     2941 gcttcgcgtt ccagatgttc gccgcgtact cggcaatggt gcgatcgctc gagaatttcc
     3001 cggagctggc cacattcacg atcgccctgc gcgcccattc ctccggatcc gcgtacagct
     3061 gcagcaatcg gcggtccgcc tccagatagg ccgcgagatc cgccaggtgc atgtagtgat
     3121 ccccgtgcgt caagagcgta tcgcccagtg gggcgaagac gcccggttca tggcggctga
     3181 agtggtccga gaagatcagg tccagcgacg cccgggtctc cggctcgttg tcatagtgcc
     3241 agcgcgggtt gtaccagccg cgactggccg ccacctggtc cgccgtcagg ccgaagagga
     3301 agaaattcct ctcgcccgct tcctgcgcca tctcgatggt cgcgccgtcc cgcgtgccga
     3361 tcgtcagggc gccgttcatc atgaatttca tgttgctcgt cccgctcgcc tcgtagccgg
     3421 cggtggagat ctggttggag acatcgctgg cgggaatcaa ctgttctgcc agggacacgt
     3481 tgtactcggg cagaaagagc acctgcagtc ggttccgcgt ggcaggatcc ccctcgatgg
     3541 tgctcgccag gctgttgatg aacttgatga cgagcttggc caaatggtag gccggtgccg
     3601 ccttgccggc gaagaagaaa gtccgaggcg tcatctcgag attcgggttc tctcggaggc
     3661 ggtggtacag caccacgatg cgcagcgcat tgaggagttg gcgtttgtat tcatgaatgc
     3721 gcttgacctg acaatcgaag atgcggtccg gatccaccgt ccgaccggat gtcgctttga
     3781 gccaggcggc aaattgcacc ttggccgcgc gcttggcctt gcggaaggca tcgcgaaatc
     3841 cccgatcgtc ggccagcgac ctgagcttgc gtagctgctc gaggtcggtg atccacccat
     3901 cgccgatggc cgcggtgatc gtctgggcga gcgccgggtt cgccagcagc aaccagcggc
     3961 gcggcgtgac gccgttggtc ttgttgttga accgctccgg gaacagctcg gccaggtcct
     4021 tgaccgtcgt cgtgcgcagt agctcggagt gaatggcggc gaccccgttg gtgctgtgcg
     4081 agccgacgat cgccaggttg gccatgcgga tgtgcttggc ggcgccttcc tccatgaggc
     4141 tcacgcgggc gacccgcccc gcgtcaccgg gaaaccggat ccgcaccgtc tccagcaggc
     4201 gccgattgat ctcatagatg atctccagat gacgcggcag cagatcctca aacaatgcca
     4261 gcggccatct ttccagggct tcgggcaaca gcgtgtggtt ggtgtaggcc agggcccgcc
     4321 gggtgaggtc ccaggcctga tcccatccaa gcttcgcgtc atccaggaga atccgcatca
     4381 actcggggac ggttagcgcc gggtgcgtat cgttgagctg gatggcgact ttctcaggga
     4441 gtgtacccca gtcggcattg ctccggcgga atcggcgcac gaggtcggcc agcgaacagg
     4501 ccacgagaaa atactcctgc acgaaacgca gcccctgccc catgctcgtg gaatcgtcgg
     4561 gatacagcac ccgggtgagg gattcggccg ccagcgtctc ggccagcgcc ccgacgaagt
     4621 cgccgtggct gaaggcctga aagtcaaagt aatccggcgc ggcggcggcc cagaggcgga
     4681 gggtgttgat cgtcttgccg ccgtagccca ccacgggccg atcgaacggg atgccgatca
     4741 gggtggtcgg ccggccggga accgggtgca agaccccgcc gcgcagctcg aacgagctgt
     4801 tcaggttgac ctccacgctc tcgtcgggac gcgcgacctc ccaggggtcc ggacgccgca
     4861 gccagttgtc cggccgttcc tgttgccacc cgtctcggat ggcctgcttg aagatgccgt
     4921 attcgtaccg cagaccgtag cccatggccg ggagctgcat cgtcgccatc gagtccagga
     4981 agcacgctgc caggcgaccg agtcccccgt ttcccaggcc ggcatctggc tcctcttcga
     5041 gcaggccgag ccaatcgagg ttcatctggg cggcggcgtc cttcacgacc ggatcgagca
     5101 gcaagttcat cacattgttg gccagcgcgc ggccgatgag aaactccatg gagagatagt
     5161 agacgcgctt ggggttttcc cgcacgtacg tgtcctccgt gagcacccac cgctgcgaga
     5221 ggacgtcccg gacggagcgg gcgatggcct caaaccgctc gcgcgggccc gcggcggtcg
     5281 ggttgacgac gttgtcgaag agcaggtgcc gctcatagag tgcatcgggg gtccccgtga
     5341 gctcgaccgg accgcacccg tattgccgga gcagcgccgc cacggcctcc gatgctgttg
     5401 ctgcttgagg agctgccgtt ttcatgctca tcgccgtatc ctttcttgga tagctcgcgc
     5461 gagagggtta ggaaacccgt ccatcatcgc ggccccgcct cgcccttctg ggcctctgcc
     5521 gccattaagc gcgccaactc gaactggtac ctgcccttca ggcagagcct ggtgccttcg
     5581 tctcacccgc gcaggaagca ctgtctgcct cgggcggcac ccccgcccct gggccgccgg
     5641 ctggcgcggg ggg
//
LOCUS       DBNGZC010000294         2298 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_122295, whole genome shotgun sequence.
ACCESSION   DBNGZC010000294 DBNGZC010000000
VERSION     DBNGZC010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_122295"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..623
                     /locus_tag="AC16G0_05420"
     CDS             333..623
                     /locus_tag="AC16G0_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020000170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HIR5099097.1"
                     /translation="MAVEETHLRDAGEGGEVTFSSLVLMFGTTALVHLGVAPDPGSGQ
                     KTPNLTQAKQVIDLLAVLQEKTKSNLTPEESGLLDSLLFDLRMRYLEIAKSG"
     gene            790..2256
                     /locus_tag="AC16G0_05425"
     CDS             790..2256
                     /locus_tag="AC16G0_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HIR5099098.1"
                     /translation="MNRLGRNTRRMGGWMILAAFLALCLPDIAGPAMAQEGRTALQTL
                     QDAFVHVAETVKPAVVNISTTQHPPSQEGRRSPQLPPSFRGPFRDFFGEDFFERFFGQ
                     PPQREQHSLGSGVIVDKRGYILTNNHVIEQADQIEVRLSDKRKFQATVMGRDSKTDLA
                     VIRIQAGTDLPVATLGNSNKIRIGEWAMAIGNPFGLDQTVTVGVISAMGRSDVGITTY
                     EDFIQTDASINPGNSGGPLVNMNGEVVGINTAIVASGRGIGFAIPISMAREIMDRLIA
                     QGKVVRGWLGVGIQDLTDELASQFGVKPGDGVLVGNVMKDSPADKGGLKTGDVLLEFN
                     NTKISDVHQLQREVAQASVGSTVRLRVLREKQPMTVSVVLGEQPTELAVARPGPQSTE
                     ATDRFGLTVQDLTPELRDQFKLGETSGAVVSAVAEGGAAGRAGIRPGDLITEANRQRV
                     KNVQELTRILGQVSPGSGLLLLVQREGNSRFVVVTPSN"
BASE COUNT          435 a          669 c          778 g          416 t
ORIGIN      
        1 cgggccctca ggctcggcgc gtcgatatgt gtggagtggg agagggagtc ggatgcaggc
       61 cgcgtttttc ggaagaaaag cggatgatca tgcccgggcc atacccaacc tggcgggtgg
      121 atacccggcg gccgacggcc tccgattgcg ctgcttccgt gccatgggtt gtcgctcagg
      181 tcccgctcga agaaccgttg cgtcgtcttg atctgatgtg agcagcctcg ccgatggaag
      241 gttctcccgg cagccagcca gatagtgctg aggcccggat aaagataaat ttgacttgaa
      301 aggtcaccaa gcgtgttgtt aaggtatttc ccatggctgt ggaagagacg catctacgcg
      361 atgctggcga aggcggagag gtgacgttta gcagtctggt gctgatgttc ggcaccacgg
      421 ctctggtcca tctcggcgtg gctccggacc caggcagcgg ccagaagacg cccaacttga
      481 cgcaggcgaa gcaggtcatc gacctcctgg cggtcctcca ggagaagaca aagagcaatc
      541 tgacgcctga ggaatccggt ttgctggaca gtctgctatt tgatctgcgg atgcgctacc
      601 tggaaattgc caagtccgga tgagagggtt gttcgccccg ttgcctgcat gcgttcacga
      661 gcgtcggaat tccagacgac ccgacagcgc gagggccgca aacgcctcgt gtggcttgac
      721 agtcccccct cgctatgtta caggaagact gtctttccgc acgcgtgttt cctacccgtg
      781 gaggtttccg tgaaccgatt gggccgtaat accagacgga tgggcggatg gatgatcttg
      841 gctgcattcc tggccctctg cctccccgac atcgctggtc cggcgatggc gcaggagggg
      901 cggaccgccc tccagacgct gcaggacgcc tttgtgcacg tggccgagac ggtcaagccc
      961 gcggtggtga acatttcgac cacgcagcac ccgccatcgc aggagggtcg ccggtcgccg
     1021 cagctaccac cgtccttccg cggccccttc cgcgactttt tcggcgagga cttcttcgag
     1081 cggttctttg gccagccacc gcagcgggag cagcacagcc tgggttccgg tgtgatcgtg
     1141 gacaagcggg gctacatcct gacgaacaac cacgtcatcg aacaggcgga tcagatcgaa
     1201 gtccgcctct cggacaagcg gaagttccag gcgacggtca tggggcggga ttcgaaaacc
     1261 gatctcgccg tgatccggat ccaggctggc actgacctgc cggtggcgac cctgggcaac
     1321 tccaacaaga ttcgcatcgg ggaatgggcc atggcgattg ggaacccctt cggtctggat
     1381 cagaccgtga cggtaggggt catcagcgcc atgggccgtt cggatgtcgg catcacgacc
     1441 tacgaggact tcatccagac tgacgcctcg atcaaccccg ggaattcggg tgggccgctg
     1501 gtgaacatga acggggaagt cgtcgggatc aacaccgcca tcgtggctag cggccggggg
     1561 atagggtttg ccatcccgat cagcatggcg cgcgagatca tggatcgcct gattgcccaa
     1621 gggaaggtag tccgaggctg gctgggggtc gggatccagg acctcaccga cgaactggcg
     1681 agccagttcg gggtgaagcc gggcgatggg gtcctggtgg gcaacgtcat gaaggacagc
     1741 ccggcggaca agggtgggct gaagaccggg gacgtcctct tggagttcaa taacacaaag
     1801 atctcggatg tccaccaact ccagcgggaa gtggctcagg cctcggtggg ctcgacggtc
     1861 cgcctgcggg tactgcggga aaaacagccg atgacggtca gcgtggtctt gggggagcaa
     1921 cccaccgagc tggcggtggc gcgcccgggc ccacaatcca cggaggccac ggatcgcttc
     1981 ggcctcacgg tccaagacct cacgcctgag ctccgagacc aattcaagct cggcgagacc
     2041 agcggcgcgg tggtttcggc tgtggccgag gggggggccg cgggtcgcgc cggaattcgt
     2101 ccgggcgatc tgatcacaga ggcgaaccgg caacgggtga agaacgtcca ggagctcaca
     2161 cggattctgg gccaggtgtc gccggggtcc ggactcctgt tgttggtgca acgagaggga
     2221 aactcgcgct ttgttgtggt cactcccagc aactgagact ccagctgccc aggtccggcg
     2281 gtgatggctg cgacgcgg
//
LOCUS       DBNGZC010000295         2457 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1980760, whole genome shotgun sequence.
ACCESSION   DBNGZC010000295 DBNGZC010000000
VERSION     DBNGZC010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1980760"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="AC16G0_05430"
     CDS             complement(<1..653)
                     /locus_tag="AC16G0_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIR5099099.1"
                     /translation="MAKTAGILIIGSEVLSGKVTDQNSPYLVRELRDLGVEVLRISTI
                     PDDVRVIAHDVREYAGQFDLLFTTGGIGPTHDDVTIEAIGTAFDRRVIRHPVLEKALR
                     RHYGDQITPAQLKMAEIPEGGRLTGEGDLTFPVLVFQNVYIFPGIPEAVRQKFDRIRE
                     QFRERPFILRRVFLQGDEGKIAEDLHAVLDSYPALQLGSYPILHKPDHNVMLTLESK"
     gene            complement(738..1181)
                     /gene="tsaA"
                     /locus_tag="AC16G0_05435"
     CDS             complement(738..1181)
                     /gene="tsaA"
                     /locus_tag="AC16G0_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006679613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HIR5099100.1"
                     /translation="MEWIGVVYNDVKEPTDEGWGGVESEVVLDETLADGLDGIEAFSH
                     VLILYWMHRAAEAEPVRMRRHPQGRQDMPDVGIFAQRAHHRPNPIGVAAVKLLRREKN
                     RLFVRGLDAINGTPVLDVKPYVPQFDAVDSPRVPEWVNRLMQGYF"
     gene            complement(1338..1874)
                     /gene="lepB"
                     /locus_tag="AC16G0_05440"
     CDS             complement(1338..1874)
                     /gene="lepB"
                     /locus_tag="AC16G0_05440"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017322156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR5099101.1"
                     /translation="MENTTQGQRKRVWEWAKSIGLALLLAFVIRTAVVEAFVIPSGSM
                     EPTLQIGDRLLGNKFWFWFADPHRQDIVIFSPPASAHLAAGALIKRVIAVEGDVVEVR
                     DSQVLVNGTPVVEPYLKERPQYTLPPTRVPQGMLFVLGDNRNASYDSHAWGFVPRNHL
                     KAKAFVRYWPLNRIGMLQ"
     gene            complement(1887..2285)
                     /locus_tag="AC16G0_05445"
     CDS             complement(1887..2285)
                     /locus_tag="AC16G0_05445"
                     /inference="COORDINATES: protein motif:HMM:NF014016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HIR5099102.1"
                     /translation="MPADAKKIGLLLVDHGSRFQEANDMLIEVAAMVKRLGVLDCVHY
                     AHMELAEPTIRQGFAACVREGATAVVVHPYFLSPGRHSTSDIPRMVAEAAKDYPGIEF
                     CVTEPLGVHPKIGEVVLERAGVPLPAGVRG"
BASE COUNT          454 a          831 c          736 g          436 t
ORIGIN      
        1 tccttggatt ccagcgtcag catcacgtta tggtcgggct tgtggaggat gggatatgag
       61 ccgagctgca aggccggata gctatccagg accgcatgca ggtcctcggc aatctttccc
      121 tcgtcgccct gcaggaagac tcgcctaagg ataaagggcc gctctcgaaa ctgctcacga
      181 atacgatcga atttctgccg gacggcctct ggaatcccgg ggaagatgta aacgttctga
      241 aagacgagga cgggaaaggt tagatccccc tccccggtca accggccccc ctcggggatc
      301 tccgccatct tgagttgcgc gggggtgatc tgatcgccgt agtgccggcg cagcgccttc
      361 tccagcacgg gatggcggat cacgcgtcgg tcgaaagcgg tcccgatggc ctcgatggtc
      421 acgtcgtcgt gggtcggacc gatcccgccg gtggtgaaca gcagatcgaa ctgccccgca
      481 tactcccgca cgtcgtgggc gatgacccgc acgtcgtcgg gaatggtgct gatccgcagg
      541 acctcgaccc ccaggtcgcg caactcccgg accagatacg gagagttctg atccgtcacc
      601 ttcccgctca gcacctcgct gccgatgatc aggatgcccg ctgtcttggc catgcctccg
      661 ccgactcctg cctcgtgcct ttgccggctg tccccggccc ttccccttac ggctgatggc
      721 cgacggctac cgccgcccta gaaatacccc tgcatcagcc gattcaccca ctcgggcacc
      781 cggggagagt ccaccgcatc gaactgaggg acgtacggct tcacgtccag aaccggcgtt
      841 ccgttgatcg catccagccc ccggacgaag agccggttct tctcccggcg gagcagtttc
      901 actgcagcca cgccgatcgg attgggacgg tggtgggccc gctgggcgaa gatgccgaca
      961 tcgggcatgt cttgtcgacc ctggggatga cggcgcatcc ggactggctc ggcctcggcc
     1021 gcccggtgca tccagtacag gatgaggaca tgggagaatg cctcgatgcc gtccagccca
     1081 tccgctagcg tctcgtccag cacaacctcg gactcgaccc caccccagcc ctcgtcagtc
     1141 ggctccttga catcgttgta caccacgccg atccattcca cgggatccgc tcctcctccg
     1201 gggcagctcg cgcccttccg gaatgccagc gaggtcgagg atatgcatgt gagaggtcga
     1261 gggacatccc catccctcgt tgccatcctg ccaggtcccg tgccaccgcc gagcaatcgg
     1321 gcggcaggac ctcaccccta ctgaagcatg ccgatccgat tcaagggcca gtagcggacg
     1381 aacgccttgg cctttaaatg gttgcggggc acgaatcccc acgcatggga atcatagctg
     1441 gcgtttcgat tatcccccag gacaaacagc atgccctggg gaacccgcgt cgggggcagg
     1501 gtgtattgcg gtcgttcttt gaggtagggc tcgacgacgg gcgtgccgtt gaccaacacc
     1561 tggctatccc ggacctccac cacgtcgccc tccacggcga tcacgcgctt gatcaacgca
     1621 cccgccgcca aatgggcgct ggcgggtggc gagaagatca cgatatcctg gcggtggggg
     1681 tccgcgaacc agaaccagaa cttgttcccc agaagccggt caccgatctg cagcgtcggc
     1741 tccatggatc cggagggaat gacgaacgcc tcgaccacgg ccgtgcgaat cacgaacgcc
     1801 aggagcaggg ccagaccgat gctcttcgcc cactcccaga cgcgcttacg ctggccttga
     1861 gtggtattct ccataggaac ctccaatcag ccccggaccc ccgcggggag cgggacgccg
     1921 gctcgctcca gcaccacctc gccgatcttg gggtggacgc cgaggggctc ggtcacgcag
     1981 aactcgatgc ccggatagtc cttggcggcc tcggccacca tccgcgggat atcactcgta
     2041 gagtggcgcc ctggcgagag aaagtatggg tggaccacca cggccgtggc gccctcgcga
     2101 acgcaggctg caaagccttg tcggatcgtc ggctccgcca gttccatgtg tgcgtaatgc
     2161 acgcaatcaa gcacgcccag tcgcttgacc atggccgcga cctcgatcag catgtcgttt
     2221 gcttcttgaa atcggctccc gtgatccacc agcaggagcc cgatcttctt tgcatcggct
     2281 ggcatgacac ccctccactt ccgctacggt gaagcgatat gatgcatcgg ctcaagattc
     2341 aggcaacgac gcctgccccc gccaggcggc tgcggatgtc cgccacgctg aggccgaaat
     2401 cggcatcgga gagcaccggg acacaaccgt cggtactgag ggactggtca aggtcca
//
LOCUS       DBNGZC010000296         3605 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_319239, whole genome shotgun sequence.
ACCESSION   DBNGZC010000296 DBNGZC010000000
VERSION     DBNGZC010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_319239"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1357
                     /locus_tag="AC16G0_05450"
     CDS             191..1357
                     /locus_tag="AC16G0_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099103.1"
                     /translation="MPSTWPEDTDFALWELDVVERQCPSCGRMMHVWDHRYRRFHTLQ
                     GPVELLCRLNHCPDPDCPGHARIKSPELEVAIALPKWAIGWDVLCWIGHRRCSRHWAI
                     PQIQGELRDDYGIHLSEDSIARYIHHYQVMLAARQQDPEVLRRQYQSAGEIILSIDGL
                     QPEKGHETLYVVRELTRKRIWFAEPLISATADEVRRLIAQAREWAESLGKPVVLWLSD
                     KQDAFVTGIAAEFPDVPHRYCDNHFLRDVAKPVLDAASHAKVQMRRKVRGLRTIEQAV
                     LARRKAETRDDLTPGDPEVTVTATAANLPPAVVDPAGGVVLDYGAAVRGILNDDQGGP
                     LPPPGLGMAEALNEVQESIQRNLGTRKGGSRRSNSAACPDASRGAWTKSRDSKR"
     gene            1375..1875
                     /locus_tag="AC16G0_05455"
     CDS             1375..1875
                     /locus_tag="AC16G0_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099104.1"
                     /translation="MIAEVAATLEPGTEDVTERPERFEALIDQFERTEDPIRHSMAAV
                     MLSFLAGLFVGEEAYEGIRDNLDQERWFRLPKSHERRIHGRRHVGVRIVVAGPTLVHA
                     LDAHAVHPEPFTADDLLPYRTAREPPCQTQALNRRKIMRKARSKKQRPVLLADLERRY
                     RDSPSS"
     gene            2023..3231
                     /locus_tag="AC16G0_05460"
     CDS             2023..3231
                     /locus_tag="AC16G0_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_207689347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HIR5099105.1"
                     /translation="MEPLSEHTIKTLREAARLLTGPKRRRFQAQVTRDYLHGSARQAE
                     RVFGWGRQTIQLGLHELRTGITCLDRYTERGRHATEEDHPQLAHDIRDLVEPHSQGDP
                     TSPSPFVYTRLTAQAVRQALIDRKGYRDEDLPCERTVRNLLNRLGYRLRHVQKTRPIK
                     KIKETDAIFAHVEAVHEQAAGAEDTLRISIDTKAKVQVGELSRDGAARGAGPVKALDH
                     DMKPVATMVPFGILEVVSGLLTILFGTTCLTSDFVVDCLEQWWQGRRKSSPRVKRLVI
                     DLDNGPETSSRRTQFVKRLVEFADKFGLVVELVYYPPYHSKYNPIERCWGILEKHWNG
                     ALLGSMETVLGWARTMTWKGQRPGVHLVERDYERGRCVDKTEMKQYEARLQRSETLPK
                     WSVVITPGAC"
     gene            3260..>3605
                     /locus_tag="AC16G0_05465"
     CDS             3260..>3605
                     /locus_tag="AC16G0_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099106.1"
                     /translation="MPAGNQSEVLDISRREALSSDELGGVVRLLLGAVTAEQKLFVLA
                     KPREALGQAGVPEREGWDPQLSARGNRVSKQLAVLLDRLAWAENFLECRGWTSLVASD
                     RLVLGPVFTRLAR"
BASE COUNT          674 a         1120 c         1185 g          626 t
ORIGIN      
        1 acgcggcttc cgagagcatc gagcgccgat tttcgcggcc gagactcacc tacaatctgg
       61 acacgccgac cccatttatt tacaacgatc cgcaacaggg ctagaaaggc ggctcctacg
      121 cgtgtaacct ctggcctggt ctacccaagc gatgaatccc agtgccgcgc gccgcgggga
      181 gatccgcacg atgccctcca cttggcccga ggataccgac ttcgccttgt gggagctcga
      241 cgtcgtcgaa cgccagtgcc cgagctgcgg acggatgatg cacgtctggg accaccgata
      301 ccgccgtttc catacccttc aggggccggt cgagttgctc tgcagactca accactgccc
      361 cgatcccgac tgccccggac acgcccggat caagagcccc gagcttgagg tcgccattgc
      421 actccccaaa tgggctatcg gctgggatgt cctctgttgg atcggtcatc ggaggtgttc
      481 acggcactgg gcgatccccc agatccaagg cgaactccga gacgactatg gtatccatct
      541 ctcggaggac tcgatcgcgc ggtacatcca tcattatcaa gtgatgctcg ctgcacggca
      601 gcaagacccc gaggtcctgc ggcggcaata ccaatcggcc ggcgagatca tcctctcgat
      661 cgacggcctc caacccgaga agggacacga gaccctctac gtcgtccggg agctgacccg
      721 gaagcggatt tggttcgccg agccgttgat ctccgccacc gcggacgagg ttcgccgcct
      781 gatcgctcag gccagggagt gggccgaatc gctgggcaag ccggtggtcc tctggttgtc
      841 cgacaagcag gacgcgttcg tgacggggat cgcggcggag ttccccgacg tgccccatcg
      901 ctactgcgac aaccacttcc tccgcgacgt ggccaagccg gtcctggacg ccgctagtca
      961 cgccaaggtc cagatgcgcc gcaaggtccg cggcctgcgg acgatcgaac aggccgtgct
     1021 ggcgcggcgg aaggccgaga cgagggacga cctcacgcca ggcgatcctg aagtcaccgt
     1081 cacggcgacc gcggcgaact tgcctcctgc cgtggtcgat cccgccggcg gcgtggtgct
     1141 cgactatggc gcggcggtgc gggggatcct caatgacgat caaggtggcc ccttgcctcc
     1201 tcccggcttg gggatggccg aggccctgaa cgaggtccag gagtcgatcc agcggaactt
     1261 gggcacaaga aaagggggtt cgcggaggag caactcggcc gcttgtccgg atgcatcgag
     1321 gggggcctgg acgaagtcaa gggacagcaa gagataatcc gaggatacgt cgaggtgatc
     1381 gcggaggtgg ccgcgaccct cgagcccggg accgaagatg tcacggagcg cccggagagg
     1441 ttcgaggcgt tgatcgatca attcgagcgg accgaggatc cgatccgcca ctcgatggcc
     1501 gcggtgatgc tcagcttcct tgccggcctc ttcgtcgggg aggaggcgta tgaagggatc
     1561 cgggataatc tcgatcagga gcgttggttc cgcttgccga agagccacga gcgacggatc
     1621 cacggtcgtc gccacgtggg ggtccggatc gttgtggcgg gaccgacgtt ggtgcatgcc
     1681 ctggatgccc acgcggtgca cccggagccc tttacggccg acgacctatt gccgtatcgg
     1741 acggcccgcg agccgccgtg tcaaacccag gcgttgaacc gccgcaagat catgagaaag
     1801 gcccgatcga agaagcaacg gccggtactg cttgccgacc tggagcggcg ctaccgagat
     1861 tcgccatcct cctagctctg ttgcggatcg ttgaaaaaaa gtaacgcttc agccgttctc
     1921 agtaaggcga taggagtgaa atagagtacc cctcctctcc ggccgaccgt atactcgctc
     1981 ttttctcatc cttcccgtgt ctttgacccg cctcggtgac ccgtggaacc gctctcggaa
     2041 cacacgatca agacccttcg cgaggctgcc cgactcctga ccggtcccaa acggcgacgc
     2101 ttccaggccc aagtcacccg cgattacctc cacggcagcg cgcgtcaagc cgaacgcgtc
     2161 ttcggctggg gacggcagac catccaactc ggtctccacg agttgcgcac cgggatcacc
     2221 tgcctcgatc ggtacaccga gcgcggacga catgccacgg aggaggacca tccccagttg
     2281 gctcacgaca tccgcgacct cgtcgagccc cactcccaag gcgatcccac gtccccatcc
     2341 ccgttcgtct acacacgcct cacggcccag gctgtccgcc aagccctgat cgatcgtaag
     2401 ggctaccgcg acgaagacct gccctgtgag cgcactgtcc gcaatctcct caaccgtctg
     2461 ggctaccgcc tgcgccatgt gcagaaaacc cggccgatca agaagatcaa agagaccgat
     2521 gccatcttcg cccatgtcga ggcggtccat gagcaggcgg cgggcgcgga ggacacgctc
     2581 cggatctcga tcgacaccaa ggccaaggtg caggtcgggg agctctcgcg tgacggtgcg
     2641 gcccggggcg cggggccggt gaaggccttg gaccacgata tgaagccggt ggcgacgatg
     2701 gtccccttcg ggattctgga ggtggtcagt ggtttgctga ccatcctgtt cgggacgacg
     2761 tgtttgacga gtgacttcgt ggtggactgc ctggagcagt ggtggcaggg gaggcgcaag
     2821 agttccccac gggtaaagag actggtgatc gatctggaca atggaccgga gacgtcaagt
     2881 cgtcggacgc agttcgtcaa gcgcctggtg gagttcgcgg acaagttcgg tctggtggtc
     2941 gagttggtgt attatccgcc gtatcacagc aagtacaacc cgatcgagcg ttgctggggg
     3001 atcttggaga agcactggaa tggggcgttg ttggggagta tggagacggt gctgggttgg
     3061 gcgcggacga tgacctggaa ggggcagcgc ccgggagtgc atttggtcga gagggactat
     3121 gagcgtggga ggtgtgtgga caagacggag atgaagcagt acgaggcccg tctgcaacgc
     3181 tccgagaccc ttcccaagtg gagtgtggta atcactcctg gagcatgtta gtggggtaat
     3241 ttatctttga ccgatagcct tgccggccgg caaccaatcg gaagttctcg atatctcacg
     3301 gcgcgaggcg ctcagtagtg acgaactcgg cggcgtggtc cgcctgctgt tgggggcggt
     3361 gacggccgag cagaagctct tcgtgctggc caagccgcgg gaggcgttgg ggcaagccgg
     3421 cgtgccggag cgggagggct gggaccccca gctcagcgca cgcggcaacc gcgtgagcaa
     3481 gcaactcgcg gtgttgctcg accgcctggc gtgggcggag aacttcctgg aatgccgcgg
     3541 ctggacgtcg ctggtggcct ccgaccgcct ggtcctgggc ccggtcttca cgcggctcgc
     3601 acgcg
//
LOCUS       DBNGZC010000297         1804 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1884044, whole genome shotgun sequence.
ACCESSION   DBNGZC010000297 DBNGZC010000000
VERSION     DBNGZC010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1884044"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1725
                     /locus_tag="AC16G0_05470"
     CDS             <1..1725
                     /locus_tag="AC16G0_05470"
                     /inference="COORDINATES: protein motif:HMM:NF014598.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIR5099107.1"
                     /translation="IGYKLYAANIDHSVIKADPAKATPAKMYNDGVDFMPASSSVLFG
                     YQFKSIAALGPIVGPIVAVQWGWLPAILWLILGVFFIGWIQDYASAVMAMRNDGLTMG
                     GLSYKLISPRARTILLSFLYFYLLLIMGAFGAIVAGLFANPKVPFGFIILTLAGVLAG
                     QMTYKWRKDLIVTTLSCVALAIFGIWLGTTGPFQAIVGAINGGKDSFILYATPYGPMK
                     WSVFFWGIVVLGFCYLGSVLPIWRFAQPVNYVAFWLVLMGIVGSIIGIFFQMPSFGDF
                     PAFTGFMAKGIIAPGFDTPLWPILFVTIACGAISGWHSLVSTSGTARQLEKETDALPV
                     GAGAMFTEGVLAVLSVVFAVTAFAGAKGNMGGYFDALKAGGPAGVFANGMASYLNVLG
                     IAKDFGVAFGSAFLVIMALTVMQLVLRFMRVASSELVGGAIPAFKNVHFGTTVGILLT
                     LMLMWTGFWSRIWILFGGSNQLFASLALVLITIWLAQTGKSYLWAFWPAAFMYVTTVA
                     ALIVTAWVSLQAAFTPGLKAAFIFGNLVAAVIGIALTILSLILAWDALHAFNKARSVK
                     LRAAAARV"
BASE COUNT          265 a          586 c          549 g          404 t
ORIGIN      
        1 atcgggtata aactctacgc cgccaatatc gatcactccg tgatcaaggc ggatcctgcc
       61 aaggcgacgc cggccaagat gtacaatgac ggggtggatt tcatgccggc gagctccagc
      121 gttctgttcg gctatcaatt caagtccatt gcagccctgg gtcccatcgt tggacccatc
      181 gtcgcggtcc agtggggatg gctgccggcg atcctctggc tgatcctcgg cgtgttcttc
      241 atcggctgga tccaggacta cgcctcggcg gtcatggcca tgcggaacga cggcctcacc
      301 atggggggcc tgagctacaa gctgatctcg ccgcgggccc gaacgatcct tctctccttc
      361 ctctatttct acctgctcct gatcatgggc gcattcgggg ccatcgtcgc aggactcttc
      421 gcgaacccaa aggtgccctt cgggttcatc attctcaccc tggcaggcgt cctggcgggt
      481 cagatgacct acaagtggcg gaaggacctc atcgtcacga ccctctcttg cgtcgccctg
      541 gcgatcttcg gcatctggct tggaaccacc ggccccttcc aggcaatcgt gggcgcgatc
      601 aacggcggga aggattcctt catcctctac gccacgccct acggcccgat gaaatggtcg
      661 gtcttcttct gggggatcgt ggtcctgggc ttctgctacc tcggctcggt cctgccgatc
      721 tggcggtttg cccagcccgt gaactacgtg gccttctggc tcgtcctgat ggggatcgtt
      781 gggtccatca tcgggatctt cttccagatg cccagctttg gggattttcc ggccttcacg
      841 ggtttcatgg ccaagggcat catcgcgccg ggcttcgaca cacccctctg gcctatcctc
      901 tttgtcacca ttgcgtgcgg cgcgatctcg gggtggcata gcctggtctc cacctcgggc
      961 accgcacgtc aactggagaa ggagaccgac gccctgccgg tgggggccgg ggccatgttc
     1021 acggaaggcg tcctggcggt gctctccgtg gtcttcgccg tcaccgcctt cgccggtgcc
     1081 aagggcaaca tgggaggata cttcgacgcc ctcaaggcgg gcggccccgc gggtgtcttc
     1141 gccaacggga tggccagcta tctgaatgtt ctcggcatcg ccaaggattt cggcgtggcc
     1201 ttcggctccg ccttcctggt gatcatggct ctcacggtga tgcagctggt cctccgattc
     1261 atgcgcgtgg ccagctccga gctggtgggc ggggcgatcc cggcgttcaa gaacgtccac
     1321 ttcggaacga ccgtcggcat cctcttgacc ctcatgctca tgtggaccgg gttctggtca
     1381 cggatctgga tcctcttcgg cggctccaac cagctttttg cgagcctggc cttggtcctg
     1441 atcacgatct ggctggccca gaccggcaag tcctatctgt gggcgttctg gcccgccgcg
     1501 ttcatgtacg tgacgacggt ggcggccttg atcgtgaccg cctgggtgtc tctccaggct
     1561 gcgttcaccc caggcttgaa ggcggccttc atcttcggga acttggtcgc cgcagtgatc
     1621 ggcattgccc tgacgatcct gtcgctgatc ctggcctggg acgctctcca cgcattcaac
     1681 aaggctcgaa gcgtgaagct gcgggcggcc gcggctcgcg tgtagtccaa caactggcgc
     1741 cccgccacgg gcagatagta ccgtcgcccg tggcggggta accgccatcg gtttgcctgc
     1801 tggt
//
LOCUS       DBNGZC010000298         2996 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1785862, whole genome shotgun sequence.
ACCESSION   DBNGZC010000298 DBNGZC010000000
VERSION     DBNGZC010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1785862"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="AC16G0_05475"
     CDS             complement(<1..773)
                     /locus_tag="AC16G0_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018099132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099108.1"
                     /translation="MSDESRSIPLAEMTLWQKTRRFVRRNPTLVAGAALLGLMAALAL
                     LASSLGTVNPVDMNPVQRLKAPGAGRWFGTDMYGRDSYSRVIYGARISLVVGLVAALV
                     SSVVGLAIGLVSGFVRQLDNVIMRVMDGIMALPDLLLAISFVALTKASVRNVIIALVI
                     PQTPRVVRLVRSIVLTLREQPFIEAAQAMGARLPRLLLRHLLPNTLAALIVQGTYVMG
                     SAVLIEAGLSFLGAGTPPEIPSWGNIMAEARSYVRVAFWL"
     gene            complement(824..1765)
                     /locus_tag="AC16G0_05480"
     CDS             complement(824..1765)
                     /locus_tag="AC16G0_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099109.1"
                     /translation="MLTYTLKRILAILPVMGIVVVFVFLLVRLAPGDPATILAGDYAT
                     PENIQKIRTGLGLDRPIHLQFAIYAGNLLRGDLGKSLHSQMPVGQLILQRLEPTVALA
                     LSTLAFAVLLAIPLGILAAWKAGSLLDRAVMVFAVGGFSIPIFWLGFLLIYVFSIRLD
                     LLPVQGYVSFREGLVPFARHLVLPTVALGMVYMALLARITRATVLEVLQSDYIRTAFA
                     KGVPSRGVLFRHALKNAAVPVVTTIGVGFALLIGGVVITESVFALPGLGRLTVDAILQ
                     RDYSIIQGITLIFALSYALVNLVVDLSYSLFDPRIRY"
     gene            complement(1830..>2996)
                     /locus_tag="AC16G0_05485"
     CDS             complement(1830..>2996)
                     /locus_tag="AC16G0_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099110.1"
                     /translation="FVLKLKEPYGLTIRALAKEYSYVPFIIPERQAVQDASKPFEGEI
                     IGSGPFMFAQKEWVPGAKTVYLKFKDYVPRKEPASFYAGGKKVNVDRVEFVVIPDQST
                     AVAALEKGEVDYVQRPHMDQWPLLRKNPNIAMYRSDVSQMQIHPNHLNPPFDNVKARQ
                     ALLAMVNQEEYLRAIAGDPENFRVCYSMTGCGGWLETDAGADLLKKQDFEKAKQLLAE
                     AGYKGEKIVVMAPSDFAPINAASLTTAAMLRKIGVNVDLQVVDWGTLVSRRGVKEPPS
                     KNPGGYHIFHTFSGAAAVKDPWGHANISTACDNGWFGWACSARASKALEDLGQLTPGT
                     DAFKKGLTEYHNTLMENVTYVPLGEFFQMSAYRKDRLSGIPETPHTVFWNLVKK"
BASE COUNT          570 a          966 c          975 g          485 t
ORIGIN      
        1 agccagaagg ccactcggac gtaggagcgg gcctcggcca tgatgttgcc ccagctcggg
       61 atctccggcg gagtcccggc ccccaggaag ctcaagccgg cctcgatcag gacggcggac
      121 cccatgacgt aagtcccctg gacgatcagg gcggccaggg tgttcggaag caaatgccgg
      181 aggagcaggc gcggcagccg ggcccccatg gcctgggccg cctcgatgaa gggctgctcc
      241 cggagggtga ggacgatgct ccgcaccagg cggacgacgc gtggcgtctg ggggatcacc
      301 agggcgataa tgacgttccg gacactggcc ttggtgaggg ccacgaacga gatggccaag
      361 agcaggtcgg gcagggccat gatcccgtcc atcacccgca tgatgacatt gtccagctgc
      421 cggacgaacc cgctcaccag accgatagcg agcccgacga ctgagctgac cagcgccgcc
      481 accaggccga cgacaagcga gatccgggcg ccgtagatca cccggctgta agaatcgcgc
      541 ccgtacatgt cggtcccgaa ccagcggccc gccccagggg ccttcagacg ctgcacgggg
      601 ttcatgtcca ccggattgac cgtcccaagg ctggaggcca agagggccag cgccgccatg
      661 aggccgagca gggccgcccc tgccaccagg gtggggttgc gccggacgaa ccggcgcgtc
      721 ttctgccaca acgtcatttc ggcaagcgga atgctgcgcg actcgtcgct catcggcgtg
      781 tcatgcattc ttagctctgc ccgtccccta agctcccggc ggttcagtac cgaatccgcg
      841 gatcgaagag ggagtaggag aggtcgacta cgaggttcac cagggcgtaa ctcaaggcga
      901 agatgagcgt gatcccctgg atgatcgaat agtcccgctg gaggatggca tccacggtca
      961 gccgccctag accgggaagt gcgaagacgc tctccgtgat caccaccccc ccgatgagca
     1021 acgcgaagcc caccccgatc gtggtcacta ccggcaccgc cgcgttcttc agggcgtggc
     1081 ggaagagcac ccccctcgat ggcacgccct tggcgaatgc cgtccggata tagtccgact
     1141 gcaatacctc caggacggtc gcccgtgtga tccgggccag cagagccatg tagaccatcc
     1201 ccaaggcgac cgtgggcagg acgaggtgcc gggcgaacgg tactaagccc tcacgaaagc
     1261 tcacgtagcc ctgcacgggc aggagatcca ggcggataga gaagacgtag atgagcagaa
     1321 accccagcca gaatatgggg atggagaatc cccccacggc gaagaccatc accgcccgat
     1381 ccagcaggga gcccgccttc cacgcggcca ggattcccag cgggatggcc agcaggaccg
     1441 cgaaggccag tgtgctcagc gccagcgcca cggtgggctc cagccgctgg aggatgagct
     1501 ggcctaccgg catctgcgag tggagagact tgccgagatc gccccgcagg agattccccg
     1561 cgtagatggc gaactggagg tggatggggc gatccagtcc tagacccgtc cggatcttct
     1621 gaatgttctc gggcgtggcg tagtctcccg ccaggatggt cgccggatcg ccgggggcga
     1681 ggcggacaag caggaacacg aagactacaa cgatccccat gacgggcagg atcgccagga
     1741 ttcgcttgag cgtgtaggtc agcatgggaa aggccccagg ctcgggacgg aggaggcggg
     1801 cggccgctcg ggccgcccga ccaccgacct tacttcttca ccagattcca gaacacagtg
     1861 tgaggcgttt ccggaatgcc gctcaggcgg tccttccgat aggctgacat ctggaagaac
     1921 tcgccgagcg gtacgtaggt gacattctcc atgagggtgt tgtggtactc ggtgagcccc
     1981 ttcttgaacg cgtcggtccc aggagtgagc tgccccagat cctctagagc cttgctcgcc
     2041 cgggccgagc aggcccagcc aaaccagcca ttgtcgcagg ccgtggagat gttggcgtgg
     2101 ccccacggat ccttgaccgc ggcggctcct gagaaggtat ggaagatgtg atagccgccg
     2161 gggttcttgg acggcggttc cttgacgccg cgccgcgaca ccagcgtgcc ccagtccacc
     2221 acctggagat ccacgttcac cccgatcttc cggagcatgg cggccgtggt aagggaggcg
     2281 gcattgatcg gggcgaagtc gctgggggcc atcacgacga tcttctcccc cttgtagccc
     2341 gcttcggcca ggagctgctt cgccttctcg aagtcctgct tcttcagcag gtcggccccg
     2401 gcatccgtct ccagccagcc gccgcacccc gtcatgctgt agcagacccg gaagttctcc
     2461 ggatcgccgg caatggcccg caggtactcc tcttggttca ccatcgccag gagggcctgg
     2521 cgggccttca cgttgtcaaa ggggggattc aggtgattcg gatggatctg catctgcgag
     2581 acgtccgacc ggtacatggc gatatttggg ttcttgcgga gcagcggcca ctgatccatg
     2641 tgcggccgtt gcacgtagtc cacctctccc ttctccagcg ccgcaacggc cgtggactgg
     2701 tcggggatga ccacgaactc cacccggtcc acgttcactt tcttcccgcc ggcatagaag
     2761 ctggcgggct ccttgcgggg gacgtagtcc ttgaacttca gatagaccgt cttggcgccc
     2821 ggcacccact ccttctgggc aaacataaag gggccggagc cgatgatctc cccctcgaaa
     2881 ggcttggagg catcctggac cgcctgccgc tcgggaatga taaacggcac gtagctgtac
     2941 tccttggcca gggcccggat ggtgaggccg tagggctcct tcagcttgag gacgaa
//
LOCUS       DBNGZC010000299         3754 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2470857, whole genome shotgun sequence.
ACCESSION   DBNGZC010000299 DBNGZC010000000
VERSION     DBNGZC010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2470857"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="AC16G0_05490"
     CDS             complement(<1..1076)
                     /locus_tag="AC16G0_05490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5099111.1"
                     /translation="MQTVGSETPQAILGRPMRIARVITRLNVGGPGRHAILLTAGMDR
                     ARFDTTLISGVVGKDEGDLSELACASGIVPLVIPELGRSIRPIHDLVTLIKLVRIFRD
                     LRPDLVHTHLAKAGALGRVAARLSGVPLSVHTFHGHVLEGYFHPAVARIFRWIEGRLA
                     RGTERIVAISPRLRDDILAMGIGRPEQIEVIPLGLDLRRFHGTPSARLREALGLARGT
                     PLLGIVGRLVPIKDHPTLFQAMAVLEANTGAPHLAVVGDGERRVELQRLASSLHLASR
                     IHFLGWRKDLEAILGELDVVICCSRNEGTPLALIEAMAAGVPVLSTDVGGVRDVVAHG
                     ETGWLVPPGDPQALAQAVRHLMAAP"
     gene            complement(1085..2023)
                     /locus_tag="AC16G0_05495"
     CDS             complement(1085..2023)
                     /locus_tag="AC16G0_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5099112.1"
                     /translation="MPRFLITGGAGFIGSNLAHALVARGESVRILDDYSTGRAQNLRG
                     IEDRIEIVRGDLRDPQAVAQAVRGIEIVLHQAALNSIPRSIKEPGPTDAVNVVGTLLL
                     LEAARTAGVRRLVYASSSSVYGDTPDLPKTEDLPLRPKAPYGVSKLAAELYCRIFPQI
                     YGLETVSLRYFNVFGPRQHPDSEYAAVIPRFLRRMLAGKRPVIFGDGEQSRDFTSVDN
                     VVEANLLASEAAQGVGDAFNIACGQASTLNQVAAWINESLGTRLEPLFEPPRPADIRH
                     SYASIRKAETVLGYRPSLGVREGLRRTVEWFQAHEC"
     gene            complement(2036..>3754)
                     /gene="asnB"
                     /locus_tag="AC16G0_05500"
     CDS             complement(2036..>3754)
                     /gene="asnB"
                     /locus_tag="AC16G0_05500"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR5099113.1"
                     /translation="NGEIYNFQDLRPDLERRGHRFRSQSDTEVILHLYEEHGPDCLRF
                     LRGMFAFAIWDGPRRQLFLARDRLGKKPLCYQFDGEAFRFASEVKAILQDPAVKVQPD
                     PARISEYLTYGYVPSPGSAFHGLRKLPPAHFLICREGRVEVSRYWGLRRDRKLERPET
                     EWCEEILTRLIEAVRLRLISDVPLGAFLSGGIDSSAVVAMMSRLSSEPVKTFSIGFEE
                     PEYDEMRFARRVAERFGTEHHELVVRPDAAAILPKLAWHYDEPFADSSAVPTYYVAQM
                     TRRYVTVALNGDAGDENFGGYDRYLANNLAASFDRLPGAGLFRAAVRWGLPLWPRGGR
                     RTSLFSRSRRFLDGLTEDPVRRYAHWFCQFSGERKEELLSPDFRASLDGADALDLLLT
                     AYKESHAPDFADATMGVDAALYLPDDLLVKVDIASMAHSLEARSPFLDHEFMEFAATI
                     PSSLKIRGRAMKYILKQALRGVLPDDILHRPKMGFGVPIDQWLRHELRDLVHDTLLGP
                     RCLERGYFRRKTVERLLDEHVSKRANWHDLLWTLLMLELWHRTYIDGDGALAAQRRNH
                     QSTALP"
BASE COUNT          675 a         1224 c         1222 g          633 t
ORIGIN      
        1 ggggccgcca tgaggtgccg cacggcctgc gccagggcct ggggatcacc gggaggaacc
       61 agccacccgg tctccccatg cgccacaacg tccctcacgc caccgacgtc cgttgagagc
      121 actggcacac ccgccgccat ggcctcgatg agggccaggg gggtgccctc gtttctggag
      181 caacagatta ccacgtccag ttcgcccagg atagcttcca ggtccttgcg ccaccccagg
      241 aagtgtatgc gggatgcaag atggaggctc gacgcgagtc gctggagctc cacacgccgt
      301 tccccgtcgc caacgactgc caggtgcggt gcaccggtat tcgcctctag gacggccatg
      361 gcctgaaaca gcgtggggtg gtccttgatc ggcacgaggc gtcccacgat gcccaggagc
      421 ggagtgccgc gggcgagacc gagcgcctcc cgaaggcggg ccgagggggt cccgtgaaag
      481 cgtctgaggt ccaatcccag gggaatgact tctatctgct cgggccggcc gatacccatg
      541 gccaggatgt cgtctcgcag ccgtgggctg atcgccacga tccgttccgt gccgcgcgcg
      601 agtcgcccct cgatccagcg gaagattcgc gcgacggcag ggtgaaagta gccctccaac
      661 acatggccgt gaaaggtgtg aaccgacaag ggcacgccgc taagacgcgc cgccacccgc
      721 cccagggcac ccgccttggc caggtgcgtg tgaacgaggt ccggccggag gtcccggaag
      781 attcgcacca gtttgatgag cgtcacgagg tcgtgaattg ggcggatgga gcgacccagc
      841 tccgggatca ccaggggtac gatcccgctg gcgcaggcca gttccgagag gtcgccctca
      901 tccttcccga ccactccgct gatgagggtc gtgtcgaacc gcgcccgatc cattcccgcc
      961 gtcaagagga tggcgtgccg acctggcccg cccacgttga gtcgcgtgat cacccgggca
     1021 atccgcatcg gccggccgag gatagcctga ggcgtttcgg accccactgt ctgcattctc
     1081 accatcaaca ctcgtgcgcc tggaaccact caaccgtccg ccggagccct tctcggacgc
     1141 cgaggctcgg ccggtagccc aggaccgtct cggccttccg gatcgaggca taggaatgac
     1201 ggatgtcggc cggacggggg ggctcgaaga ggggttctag acgcgttccc aggctctcgt
     1261 tgatccaggc tgccacctgg ttcagcgtgc tggcctgccc gcaggcaata ttgaaggcgt
     1321 ccccgacgcc ttgggccgcc tccgacgcca gcaggtttgc ctccaccacg ttgtccacgg
     1381 acgtgaaatc tcgcgactgc tcgccgtccc cgaagatcac gggacgcttg cccgccaaca
     1441 tccgccggag gaagcgcggg atgaccgccg catactcgga atcgggatgc tgtcgcgggc
     1501 cgaagacgtt gaagtaccga aggctcaccg tctcgaggcc ataaatctgg ggaaagatcc
     1561 ggcagtacaa ttcggcggcg agcttcgaga ccccataggg ggccttgggc cggaggggga
     1621 ggtcctcggt cttgggaagg tccggcgtgt cgccgtacac cgaggaggaa gaggcgtaca
     1681 cgagcctccg cacccccgcc gtccgggcgg cctccagcag aaggagtgtc ccgaccacgt
     1741 tcaccgcgtc ggtcggtccc ggctccttga tcgaacgggg tatcgaattg agggcggcct
     1801 ggtgcaggac gatctcgatc cctcggacgg cttgggctac cgcctgcgga tcccgaaggt
     1861 cgccgcggac gatctcgatc cggtcctcga tccctcggag gttctgcgcc cggccggtgg
     1921 agtagtcgtc taggatgcgg accgattccc cccgtgccac cagggcgtgc gcgaggttcg
     1981 acccgatgaa ccccgctccg cccgtgatca agaaacgcgg catgatctca cctcgttagg
     2041 ggagtgctgt tgattgatgg tttcgccgct gggcggccaa ggctccgtcc ccgtctatgt
     2101 acgttcggtg ccacaattcc agcatcaaga gcgtccagag caggtcgtgc cagttggctc
     2161 tcttgctgac gtgctcgtcc aggagcctct ccacagtctt ccggcgaaag tacccgcgct
     2221 cgaggcatcg ggggcccagg agagtatcat ggaccaaatc gcgaagctcg tggcgcagcc
     2281 actggtctat gggcacgccg aatcccatct tcggccggtg caggatgtcg tcgggcagca
     2341 ccccccgcag ggcctgcttc aggatgtact tcatggcccg gccccggatc ttcaggctcg
     2401 aaggaattgt ggccgcgaac tccatgaact cgtggtcgag aaacggcgag cgagcctcca
     2461 gggaatgggc catgctggcg atgtctacct tgaccagaag atcatctggc agatacagcg
     2521 ccgcatccac gcccatcgtg gcgtccgcga aatcgggggc atggctctcc ttgtaggctg
     2581 tcagcaagag gtcaagggcg tcggccccgt caagcgaagc gcgaaagtcg gggctcagca
     2641 gttcctcctt gcgttctccg gagaactggc agaaccagtg cgcgtatcgt cgtactgggt
     2701 cctcggtcaa gccgtccagg aatcgacgac tgcgggagaa gaggctggtc cgccggccgc
     2761 cgcgcggcca cagcggaagt ccccagcgga cagcggcccg gaacaacccg gcacccggca
     2821 gacgatcgaa agacgcggcc aggttgtttg ccaggtatcg gtcgtacccc ccgaagtttt
     2881 cgtctcccgc atccccgttc agggccaccg tcacgtaccg gcgcgtcatc tgggccacat
     2941 agtaggtggg gaccgccgac gaatccgcga agggctcgtc gtagtgccag gcgagcttcg
     3001 gcaggattgc ggctgcgtcc ggccggacca cgagttcatg gtgctcggtg ccgaatcgct
     3061 cggcgactcg acgggcgaat cgcatctcgt catactcggg ctcctcaaag ccgatcgaga
     3121 acgttttgac cggctcgctg gagagcctgc tcatcatggc cacgacggcg ctactgtcga
     3181 tcccgccgct caagaaagct cccaggggga catcgctgat caaccggagg cggacagctt
     3241 caatgaggcg ggtcaggatc tcctcgcacc attccgtctc ggggcgctca agctttctat
     3301 cgcgacggag cccccagtag cgggagactt ccacgcgtcc ttcccgacag atcaggaagt
     3361 gcgcgggcgg aagctttcga aggccgtgaa acgcggatcc tggactcggc acatagccgt
     3421 aggtcaagta ctctgagatc cgtgccggat cgggctgcac cttcaccgcg gggtcctgaa
     3481 ggatggcctt cacttccgag gcgaagcgga atgcttcgcc gtcgaactgg tagcagagcg
     3541 gcttcttccc cagccggtcg cgagccaaga aaagctggcg gcgcggaccg tcccagatgg
     3601 cgaaggcgaa catgcctcgg aggaacctga ggcagtccgg accgtgctct tcatacaggt
     3661 ggaggatcac ctcggtgtcg ctctgcgagc ggaagcgatg ccccctgcgc tcgaggtcgg
     3721 gccggagatc ctgaaagttg tagatctctc catt
//
LOCUS       DBNGZC010000300         4341 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1492210, whole genome shotgun sequence.
ACCESSION   DBNGZC010000300 DBNGZC010000000
VERSION     DBNGZC010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1492210"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..1662)
                     /locus_tag="AC16G0_05505"
     CDS             complement(292..1662)
                     /locus_tag="AC16G0_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR5099114.1"
                     /translation="MRGTGAQGDAINVLVVDDERPTRLLMEKELPRNGFDVTTAESGE
                     QALEHLRDQDFDVVLLDLKMPGIGGMEALRRLRESGVSAEVVILTGHPDVNTAIEAMK
                     LGAYDYLTKPFKLAEVEAVVRRAVERKRLQKENLALRRMVAQRVPLTPIVGQSQAMTA
                     VLERIERIASSDANVLIEGESGTGKGLIAQAIHGKSARAAGPFLVINCSGFQDQLLES
                     ELFGHEKGAFTGAGSLKQGLFEVADRGTLFMDEVAEMSPAMQAKLLQVLDTRELRRVG
                     GTRVHRVDVRIVAATNKDLCRELKEGRFREDLYYRLNVVNVTLPPLRERREDIPLLIQ
                     HFLRQLQGSGKRDKVILPEALRLLQDYAWPGNVRELANAVERLLILAPGEVIGPEDLP
                     PNIRSGIAAGSSHPGGPASLSEIERLHIIRVLSNTGGKKMQAARLLGIDLKTLNKKIR
                     DYRIPM"
     gene            complement(1664..3466)
                     /locus_tag="AC16G0_05510"
     CDS             complement(1664..3466)
                     /locus_tag="AC16G0_05510"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5099115.1"
                     /translation="MPHTRSAKTKDTRSRGKSSAPVEQGVILQELLAYLRESGEQLRQ
                     AWRAEMDARGLLKGLTQQELEAEPATIYEALVHCLETGRYHSAQAYARAVAERGVIRG
                     IGAKQVIGGLLALRDVCGRGLHHKFRRRWDELMQALDLYEPVVNRILTIVALAFVQER
                     EKAVGRGREQLAALVEGGMVLAVERSLDSVLQRIVEVACKLLQARYGALGVLDGKGGL
                     ERFVTSGMDEEVRARIGSLPVGKGILGVVVREARPIRLRDLTQDPRAHGFPSHHPPMR
                     SFLGVPIICKGRIFGNLYLTEKQEAEEFSKEDERLAMTLAAQAGIAIENASLYEELRR
                     SYEELKQSQALLVRQEKLASLGRLAAGVAHELNNPLSSVAGFAEALQRRAAEGGGAGH
                     FPLADCQEYLEVIQAEVARAAAIVRRLLDFSRQREPAFGPLDLQSVVTDAVSFVKRQA
                     SLANQQISLAPFPDAFEVRGDVQMLQQVFLNLLTNALDAVATGGEVRISAEHVGLEKG
                     RPWVAVHVADNGTGISTEDLARIFDPFFTTKEVGKGTGLGLAICQSIVEQHGGTIDIR
                     SEGPGKGTVVTVRLPLARTAGPSAEASAVRGGAG"
     gene            complement(3649..4005)
                     /locus_tag="AC16G0_05515"
     CDS             complement(3649..4005)
                     /locus_tag="AC16G0_05515"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5099116.1"
                     /translation="MRKCVLAGGLIGAVLLIAGVGDARAQAQNSGVEVVLDNDSVEVV
                     LLTFQPGEVVEQHVNPEPELGIMLEGELTLITPAGREILKPGTVRWLAPLTPHETRNE
                     GATPVKMWALLLKQSK"
     gene            complement(4126..>4341)
                     /locus_tag="AC16G0_05520"
     CDS             complement(4126..>4341)
                     /locus_tag="AC16G0_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5099117.1"
                     /translation="MFSWNAFNYPLMLAGRQTFPVTVGAIQFISYEQVLWGQMAAASL
                     VAVLPQLVLSLFVQKYIVRGLTMGAVR"
BASE COUNT          743 a         1509 c         1293 g          796 t
ORIGIN      
        1 aaatcattgt aaatacgcag agtataagta ttattgtgtc ccaattatcc tgacctcccc
       61 tgacgcaacg caggagtttc tcccaggggg tcggagtttc tcctgggaaa actgcgtcag
      121 cgtccatgag gcgaaatcct cagaggacgc ctcccaccgg tgtcaccgat acgaccccac
      181 tgaaaggatc tgacgaatct gccgcatgcg ggaatccgga agataggttt tacaggtgga
      241 tgaggcccag gcaggttgtt ccagtgccca ttgcgcaaac aaccagggag gctacatggg
      301 gattcgatag tccctgatct tcttgttcaa cgtcttgagg tcgatgccaa gcaggcgggc
      361 ggcctgcatc ttcttgcctc cggtgttcga gagcacccgg atgatgtgga gccgctcgat
      421 ctccgagagt gaggcgggtc cgccgggatg actcgatcct gccgctatgc ccgaccggat
      481 gttcgggggg agatcttccg gtccgatgac ctctccgggt gccaggatga gaaggcgctc
      541 cacggcgttg gcgagttcgc gcacgttccc cggccacgcg tagtcctgca agagtcggag
      601 agcctcgggc aagatgacct tatcccgctt cccgctccct tgcagttgtc gcaggaagtg
      661 ctggatgagc agcgggatgt cttcccgccg ctctcggaga gggggaagcg tcacgttgac
      721 gacattcaaa cggtagtaaa ggtcctcacg aaaccgaccc tccttgagct cccggcatag
      781 atctttgttg gtggccgcga cgatgcgcac gtccacgcgg tggacccgcg tgccccccac
      841 ccgacgcagc tcccgggtgt ccagcacctg cagcagcttg gcctgcatgg ccggactcat
      901 ctcggccacc tcgtccatga acaacgtgcc gcggtccgcc acctcgaata gaccctgctt
      961 gaggctgccg gcgccggtga aggcgccctt ctcgtgcccg aagagttcgc tctccaggag
     1021 ctgatcctga aagccgctgc agttgatcac gaggaacgga ccggcggcgc gcgcgctctt
     1081 gccatggatg gcctgggcga tcagcccctt gccggttccg ctttccccct cgatgaggac
     1141 gttggcatcg ctcgaggcga ttcgttcgat gcgctccagg acggccgtca tggcctggct
     1201 ctgtcccacg atgggcgtga gggggacccg ctgcgccacc atgcgccgga gggcgagatt
     1261 ttccttctgc agccgtttcc tttccacggc cctccgcacc accgcctcga cctcggccag
     1321 cttgaacggc ttggtgaggt agtcgtacgc gccgagcttc atggcctcga tggccgtgtt
     1381 cacgtcgggg tggcctgtca ggatcaccac ttcggcggac accccggatt cccggaggcg
     1441 cctaagcgcc tccatgccgc cgatccccgg catcttgagg tccaggagga cgacatcgaa
     1501 gtcctgatcg cgtaggtgct ccagcgcctg ctcgccgctc tccgcggtgg tgacgtcgaa
     1561 gccgttccgc ggcagttctt tctccataag cagacgggtg ggcctctcgt catccacgac
     1621 gagcacgttg atggcatccc cttgcgcccc tgtccctctc atcctagccc gccccccccc
     1681 tcacggctga tgcctccgcc gacgggcctg ccgtcctggc cagggggagc cgcacggtaa
     1741 caacggtccc cttccccggc ccttcgctac ggatatcgat ggtccccccg tgctgctcga
     1801 cgatgctctg gcagatggca agccccaggc ccgtcccctt ccctacctcc ttggtggtga
     1861 agaacgggtc gaagatccgg gcgagatctt ccgtcgagat gcccgtgccg ttatccgcga
     1921 cgtgcaccgc cacccagggg cggccctttt cgaggcccac atgctcggcg gagatccgca
     1981 cctcccctcc ggtcgccacg gcatccaggg cattcgtcag gaggttcagg aacacctgct
     2041 gcagcatctg aacgtcgccc cgcacctcga acgcgtcggg aaacggggcc aggctgatct
     2101 gctgattcgc caggctggcc tgccgcttca cgaaggagac ggcgtcagtc accacggact
     2161 ggaggtccag cgggccgaag gccggctcac gctgtcggga gaaatccagc aaccggcgaa
     2221 cgatggccgc cgcgcgcgcg acttcggcct ggatcacctc caggtactcc tggcagtcgg
     2281 cgagggggaa gtgccccgcg cccccccctt ccgcggcgcg gcgttgcagg gcctcggcga
     2341 acccggccac cgaggagagg ggattgttga gttcgtgggc gactccggca gccaagcgtc
     2401 cgagggaggc gagtttctct tgtcggacga gcagggcctg ggactgcttc aactcctcgt
     2461 aggagcgacg gagttcctcg tagaggctgg cgttctcgat ggcgattccc gcctgggcag
     2521 ccagggtcat ggccagccgt tcgtcctcct tgctgaactc ttcggcttcc tgcttctccg
     2581 tgaggtagag attcccgaag atcctcccct tgcagatgat gggcaccccg aggaacgaac
     2641 gcatgggggg atggtgcgag gggaatccat gggccctggg gtcttgggtg aggtcccgca
     2701 atcggatggg tctcgcctcc cgcaccacca cccccaggat ccctttgccc accggcaggc
     2761 tgccgatccg cgcccgcact tcctcgtcca tcccgctggt gacgaaccgc tccagccccc
     2821 ccttgccatc cagaaccccc aaggctccat accgcgcctg caggagcttg caggccacct
     2881 cgacgatccg ctggagaacg gaatccaagg accgctccac cgccaggacc atccccccct
     2941 cgaccagcgc ggccaactgt tcgcggccac ggcccaccgc tttctcccgc tcctgaacaa
     3001 aggccagcgc cacgatcgtg aggatccgat tgacaaccgg ctcgtagagg tcgagggcct
     3061 gcatcagctc gtcccatcgt cggcggaact tgtggtgcaa cccacggccg cagacgtccc
     3121 gcagggccag caacccgcct atcacctgct tggccccgat cccgcggatg accccgcgtt
     3181 cggctacggc gcgggcgtag gcctgggcgc tatgataccg tcccgtctcc aggcagtgga
     3241 ccagcgcctc gtagatcgtg gcgggctccg cctcgagctc ctgctgcgtg agccctttga
     3301 gtaatccccg cgcatccatc tcggcgcgcc acgcctgtcg gagttgctcc ccgctctccc
     3361 gaagatacgc caggagttcc tgcaaaatca ccccttgctc gacgggagca gatgattttc
     3421 cccgagagcg agtgtccttg gtcttggccg agcgcgtgtg cggcatatcg gagtctcagg
     3481 tcatctcatc ccggctagcg ggccgccgac ccttggaatt gaagacccgc gcgccacccc
     3541 tgacccccgc gacagcttag cttacagact ccccggcacg ctgtcgacgg caaacctcgg
     3601 cgcccgggca ctccgccaag aaggcacagg gcttccaatg agaatgattc acttggactg
     3661 cttcagcagg agcgcccaca tcttgaccgg ggttgcgccc tcgtttctcg tctcgtgagg
     3721 cgtcaacggg gcgagccagc ggacggtccc aggcttgaga atctcccggc cagccggcgt
     3781 gatgagggtc agctcccctt ccagcatgat tccaagctcc ggctcggggt tcacgtgctg
     3841 ttcgaccacc tcccccggct ggaaggtaag gagcaccact tcgaccgagt cattatccag
     3901 gaccacctca acaccgctat tctgggcttg ggcccgcgca tcccccaccc ccgcaatcag
     3961 cagaactgcg ccgatgagtc cccccgccag tacgcacttt ctcatcatcg tctcttgcct
     4021 ccctcacctc gatgaacgct atcccgagca gccaccctgc ccccttctgc ggggttgggc
     4081 cagacacttg cgtgcgcctg gcgacgccac acgccggtgt ggtccctagc ggaccgcgcc
     4141 catggtaagc ccccggacga tgtatttctg gacgaagaga ctcaacacca gctgtggcaa
     4201 gacagccacc aaggatgcgg cggccatctg gccccacaac acctgctcgt aggagatgaa
     4261 ttgaatggcc cccaccgtca cgggaaaggt ctgccgccca gcaagcataa gcgggtaatt
     4321 gaaggcgttc cagctaaaca t
//
LOCUS       DBNGZC010000301         2250 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_416031, whole genome shotgun sequence.
ACCESSION   DBNGZC010000301 DBNGZC010000000
VERSION     DBNGZC010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_416031"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..744
                     /locus_tag="AC16G0_05525"
     CDS             202..744
                     /locus_tag="AC16G0_05525"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR5099118.1"
                     /translation="MKQGDIAIPTTQREIRYLTAEELTRLRHYAEARALQAKESGSVT
                     AIRSWALLDTLRSSGLRASEVASLQVRDCLLGYCQAPLVLRQGKGDKQRDVFIPQELR
                     GRLKAFLAWKRDRGEDVSDSASMFAGHRGPLTRNGVWRAANGLMAAVRLDSRDATNFY
                     RHTYATHLYRVSGHDLEVVQ"
     gene            1587..1877
                     /locus_tag="AC16G0_05530"
     CDS             1587..1877
                     /locus_tag="AC16G0_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099119.1"
                     /translation="MIVGFAWGGWVTGGTAQTMADKIAQDAVVKRLAPICVLQFNRDP
                     GKDQKLKELTATDSWQKSDYVEKQGWATMPGEEKPDSNIASECARLLTLVSK"
BASE COUNT          447 a          710 c          720 g          373 t
ORIGIN      
        1 tgtcaagcca aaactcgcaa tacgaggctg agagcatggg accttggagc ggggaggttg
       61 gcaaaacgta gcacaccgat gcgcgcggac gcaacccccg cgcaactgga atgtggcgac
      121 gaccaccagg ggcggggggg ttgcagccga ccgacggcct attgacctgg agctggctat
      181 tctcgctcgc agcgcccaag tatgaagcaa ggagatatcg ccattcccac cactcaacgc
      241 gagatcaggt acctaaccgc cgaggaactg acccgtcttc gccactatgc cgaagcacgg
      301 gccctccagg cgaaggagag tggctccgtg acggccattc ggtcctgggc attacttgac
      361 acgctccgat cctccggcct ccgggcctcc gaggttgctt ctctccaagt gcgtgactgc
      421 ctcctcggat actgccaggc gccgctggtg ctccgccagg gcaaggggga caagcagcgc
      481 gacgtcttca tcccccaaga actgagaggc cgcctgaaag ccttcctcgc gtggaagcgg
      541 gaccgcggcg aggatgtctc ggactctgcc tcgatgttcg cagggcatcg aggtcccctc
      601 acccgcaacg gggtctggcg tgcggccaat gggctcatgg cggcggtcag gctggattcg
      661 cgggacgcca cgaacttcta tcgacacacc tacgcgactc acctgtatcg ggttagcggc
      721 catgacttgg aggtcgtgca atagcagctc gggcacgcat ccatggccaa catctctccg
      781 aagacactcc tctctctggc cttcctcacg cccccaccgg gatgctcgaa atcacttgtt
      841 tttccgtgct accccccagt tcaccaaggg agaacggagc cgaacgcacg gtcgtatccc
      901 cagccagata cgcccgctgg tggcagcacc tctcactttc accattcagg ggatggcatt
      961 ggtggcgacg cggcgtcccc agtgaccact ggccgtcccc gcgggagacg acagcctatg
     1021 gaaagtcggt gagcgcggca cgggatcgga cgcggcgggc aggagccacg aagagtggca
     1081 gcatcgggcg gtgtacgaca ggccacaatt tccgccggag gtgcggagag aagcaggcgc
     1141 ggtcaagaag ctgtttacgc gccggggtga tcccggcggc aatccggcca gccgcccgag
     1201 cggcgccctc cctccgaacg gtcagagtgt ctccgatatt tcggaccttt cctgcctgca
     1261 cttccccgga ggccgcgggg gaacatatct ttcaaccccg cgctcccgca ggctgcagcc
     1321 ctcgttgtgc cgcccggtga cccgaatgga tcggcccttg cacggtcact gcgtagcggg
     1381 gcggggtcct ttccgcagta cccgcagaga gccagtcacc agcgtcgcag tgccgtcgcg
     1441 gcaggaggag aagaaccatg gagcaagatc ggggcgatca aggaagagag caaaagcgga
     1501 aggtaactct tggggatcga tggcgtgaag tccgactgac gaagacggcc gttttctggt
     1561 catgcgtcgc ctcgatggtt ctcaccatga tcgtaggctt cgcgtggggt ggatgggtca
     1621 cggggggcac tgctcagacc atggccgata agatcgcgca ggatgccgtc gtcaagcggc
     1681 tggcaccgat ctgtgtcctc cagttcaacc gggatcccgg aaaggaccag aagctcaaag
     1741 aactcacggc cacggatagt tggcagaaga gtgattacgt cgagaagcag ggttgggcga
     1801 caatgcccgg agaggagaag cccgatagca acattgcttc ggaatgtgcc aggctcctca
     1861 cgctggtcag caagtagggc gcccacaagt ctcgtgttgc aggagcagcc aggatcgcac
     1921 ctggaaagga gaaccacgat acacctgagc ccttcgggcc tggcgtagct ccgccgcaag
     1981 gcgctcccgg cccccggagg gattgctcca gacctcctcc ataatcaggg gccggagatc
     2041 agcggggcga ggcgatcgca tcgtgagccc ggctgatctc cagaaatctg cccagaaggt
     2101 cgcgggcatg gtcttgggca cagcccgtcg acccgcggcg gggttatttt cgccataggc
     2161 ccccgcgttg gcccgagcga ttcgacgggg agaccaagct cctattccca accagctttc
     2221 cgggggccag cagcagcgag tcgccattgc
//
LOCUS       DBNGZC010000302         1717 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1981436, whole genome shotgun sequence.
ACCESSION   DBNGZC010000302 DBNGZC010000000
VERSION     DBNGZC010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1981436"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="AC16G0_05535"
     CDS             <1..636
                     /locus_tag="AC16G0_05535"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5099120.1"
                     /translation="SQLEETAAAVERLGRKSVVAVADIRESGEVEAAVRATVKALGRI
                     DILFNNAGTNVRKPVVEMTDEDWHTIMETNVKGIFVVARAVARQMIAQQAGRIINMSS
                     MSAVSTERDKVVYASSKGAVMQFTKGLALELAPHGINVNAIAPGYMLTPLVKGYLEAD
                     AERYTRILQRIALGRIGQPEEIGGALVFLASDASRYITGTTIAIDGGWTAS"
     gene            845..>1717
                     /gene="metF"
                     /locus_tag="AC16G0_05540"
     CDS             845..>1717
                     /gene="metF"
                     /locus_tag="AC16G0_05540"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009370164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HIR5099121.1"
                     /translation="MHIREILHRQQPCFSFEFFPPRTTEAAEQLYRTMSDLVPLRPSY
                     VSVTYGAGGSTRELTHGLIIRIHEHARLTVVAHLTCVGSTREEIEEILREYEAHGIEN
                     VMALRGDPPQGEPFFRPTAGGFAYAVDLVSFVKKHHPDMGIGVAGFPEGHPETPNRLK
                     EMEYLKAKVDAGADYICSQLFFDNRDFYDFCERCEIAGIRLPIIAGIMPVTSLKGMQR
                     MAELALGARFPAKLTKSLVRAKDDEYVERVGIHWATEQVRDLIDNGVKGVHFYTLNRS
                     KATLRIYETLGLKNV"
BASE COUNT          322 a          528 c          577 g          290 t
ORIGIN      
        1 agtcagctcg aagagacggc ggccgccgtg gagcggctcg gccggaagag cgtggtggcc
       61 gtcgccgaca ttcgtgaaag cggtgaggtg gaggcggccg tccgagcgac cgtgaaggcc
      121 ctcggccgga tcgacatcct gttcaataac gccgggacga acgtacggaa gcccgtggtg
      181 gagatgacgg acgaggactg gcacaccatc atggagacca acgtgaaggg gatctttgtg
      241 gtggctcgtg ccgtagcccg gcagatgatc gcccagcagg ctgggcggat catcaacatg
      301 tcctccatgt cggccgtgtc gacggagcgg gacaaggtgg tgtatgcctc cagcaagggg
      361 gccgtcatgc agttcacgaa ggggctggcg ctggagctcg cgcctcacgg catcaacgtc
      421 aacgccatcg ccccgggata catgctgacg ccgctggtca agggatacct ggaggcggac
      481 gcggagcggt acacgcgaat ccttcagcgg attgcccttg gacggatcgg ccagccggag
      541 gagatcggcg gcgccctggt gttcctggcc tcggatgcct cccgctacat taccgggacc
      601 acgatcgcca tcgatggagg atggaccgcc tcgtagggcc gatgcccgtc agtttccgga
      661 gggcgtgggg aatcggccaa ggcgataccg gcgaatcacg ccgtgcatgc gccatcagcc
      721 ggcctggttt cccgcctcca gggtagtccg cctccaatcc tccccttccc tggcgctaat
      781 cgttatggta ctctgcgcaa ccgggcgcac ctaagctgat gacggaggga cgcggcgact
      841 cgccatgcac attcgagaga tcctgcaccg gcagcagcca tgcttcagct tcgagttctt
      901 tccccctcgg accacggaag ccgcggagca gttgtaccgg accatgtcgg atctggtccc
      961 gctgcgaccc tcctacgtca gcgtgacgta cggcgccggg ggatccacgc gggagctgac
     1021 ccacggcctc atcatccgga tccacgagca cgcgcgcctc accgtcgtcg ctcacctgac
     1081 ctgtgtggga tcgacgcgag aggagatcga ggaaattctc cgagagtacg aggcgcatgg
     1141 gatcgagaac gtcatggcgc tgcggggtga ccctccccag ggggagccgt tcttcaggcc
     1201 gacggccggc ggattcgcgt atgccgtcga cctcgtgtcc ttcgtcaaga agcatcatcc
     1261 tgacatgggc atcggcgtgg cgggattccc cgaagggcac cccgagacac ccaaccggct
     1321 caaggagatg gagtacctga aggccaaggt ggacgccggg gcagactaca tctgcagcca
     1381 gctcttcttc gacaaccggg atttctacga tttctgcgag cgctgcgaga tcgcggggat
     1441 ccggctgccg atcatcgcgg gcatcatgcc ggtgacctct ctcaagggca tgcaacgcat
     1501 ggcggagctg gccttgggtg cgcgcttccc ggcgaagctg acgaagtccc tggtccgggc
     1561 gaaggacgat gagtacgtgg agagggtcgg catccactgg gcgacggagc aggtcaggga
     1621 tctcatcgat aacggggtga agggggtcca cttctacacc ctgaatcggt ccaaggccac
     1681 gctccggatc tacgagaccc tgggtctcaa gaatgtt
//
LOCUS       DBNGZC010000303         2994 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1003547, whole genome shotgun sequence.
ACCESSION   DBNGZC010000303 DBNGZC010000000
VERSION     DBNGZC010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1003547"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            702..2612
                     /locus_tag="AC16G0_05545"
     CDS             702..2612
                     /locus_tag="AC16G0_05545"
                     /inference="COORDINATES: protein motif:HMM:NF028511.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HIR5099122.1"
                     /translation="MKLQSKFNLAIVSIFLVVAVGIAIVTVNYVNTKTIREAENRVRI
                     YARAAWEIHESKVGRFRAASEILAQDPMIPKLFTEPRNERSLAAARKYLETVRQDQGM
                     DILNLVAPDGTVILRTRAPYNAGDKLAADPLIKLIATTGQARSGDMILQLERLEAEGA
                     GLLERCMAVAGESRGMLSGTAVPVRQGKDVVGIIQAGSLLNGAVEKVDRIRDAVFADE
                     RYRNKPVGTATIFMGDIRISTNVVDSNGRRAVGTRASPEVTDRVLRQGGSWTGKAFVV
                     DTWYISQYDPIRDPEGKVIGMLYVGELEQKYLDMRTRAVAQHLAIILGGLILALLVFF
                     WVVRSILRPVYKLAEATQLLSDGDLNHRVDVESKDEVGALSAAFNHMAETLEGQRQEI
                     VNRQQELERLSSELKAINTNYVEMLGFVTHELKNPLASAMMSLYTVKDGYLGELNPAQ
                     LKSLGSVAQSLDYFHDMVKNYLDLSRLEKGELEVAKRQVPLHARVVRPVLDALEREMR
                     ERRFVLQDHIPDDAILTTDENLLRIVYDNLLSNALKYGREGGTIQLDYFANHSQVVLG
                     VKNDGAGIPAEKLPLLFEKFSRLDTPEHSIKKGSGLGLYICREIVRKMGGEIWVDSRP
                     GEWVTFSFSLPK"
     gene            2631..>2994
                     /locus_tag="AC16G0_05550"
     CDS             2631..>2994
                     /locus_tag="AC16G0_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5099123.1"
                     /translation="MAKKILVIDDDVQLVDTVKTLLQSVGYEVASAYQAEKGVELARQ
                     ITPDLILLDVMFAGPPGPDGIEMSRRIQQDTALKDIPVIILSGVRKVLDLPFKIGPDE
                     TWMPVKTFLEKPIKPDRLL"
BASE COUNT          586 a          924 c          989 g          495 t
ORIGIN      
        1 gcgacggccg ccacggcgcc cagacccggg ctcacgcccg atcccctccc gcagagcgca
       61 aggagaattc ggcaagcgct cctcgctgtt caatcgttgt cagtccctgc atgtgactcc
      121 tggcgacccc cctgtcgagg gggcaggaac gtgtggaagc cgagatccca ccgcgagccg
      181 gaccgttcga gcggacggac ggtaacacac gccttgggtc tggacaacac ggaacgagcg
      241 gcgagagagt gctggatcag gggttgccgc gaccacccgg atcgccgccc tccctgtctt
      301 ttcgaaacgc cggcctgccc tcccttgcgg gcggagacgc ccattatggg acaatgtgtc
      361 cacccggacg tccccttacc gaccgcgcta ggcggggaac tcgcgacttt tgctgggatt
      421 gactctccaa gcgggatccc gtgttttggc cctctggttg ctagtcatct ccctagctcg
      481 tcgtgtgggc accgcacgcg accggataat caggctgtcc cggatgcgca gcacgaccca
      541 ttgccaggat ggcgatcggg tgtaatgtcg gttccgcctg ccgcagagtg gcgaagcctc
      601 gaataccacc catcggccca cctgctgtaa cgacgccaag ctgagaatcc tcgtattccc
      661 tgcaagcttg gagtgcacga agtgtgggtg agtgagcggc gatgaagctc cagagcaagt
      721 ttaacctcgc catcgtcagc atattcctgg tcgtggcggt tggcatcgcg atcgtcaccg
      781 tgaactatgt caacaccaag acgattcggg aagccgagaa ccgggtcagg atctacgccc
      841 gggcggcctg ggagatccac gagagcaagg tcggccggtt ccgcgcggcc tcggagatcc
      901 tggcacaaga ccccatgatc ccgaaactct tcaccgagcc gcggaacgag cgatccctgg
      961 ccgcggcgcg aaaatatctg gagaccgtcc ggcaggatca gggcatggac atcctgaacc
     1021 tcgtggcccc cgacggaacg gtcatcctcc ggacgcgcgc accctataac gcgggcgaca
     1081 agctggcggc cgatcccttg atcaagctga tcgcgacgac cgggcaggcc agatccggcg
     1141 acatgatcct gcagctggag cgcctggagg cggaaggggc tggcctgctg gagcgatgca
     1201 tggcggtcgc cggagagtcc cgcgggatgc tctcaggcac ggcggtgcct gtccgtcagg
     1261 gtaaggacgt ggtcggcatt atccaggccg gaagcctcct gaacggcgca gtggagaagg
     1321 tcgatcgcat ccgggacgcc gtgtttgccg acgagcgcta ccggaacaaa cccgtcggga
     1381 cggcgaccat cttcatgggg gacatccgga tctcgacgaa cgtggtggac agtaacgggc
     1441 ggcgggcggt gggcacccgc gcgtcacccg aggtcaccga ccgggtgctc cgccaaggag
     1501 gatcctggac cggcaaggcg ttcgtcgtgg atacctggta catctcccag tacgatccca
     1561 tccgggaccc cgagggcaag gtgatcggca tgctctacgt cggggagctg gagcagaagt
     1621 acctggacat gcgcacccgg gctgtggccc agcacctggc catcatactg gggggcttga
     1681 tcctggcatt gctggtcttc ttctgggtgg tgcgcagcat tctgcgcccg gtttacaagc
     1741 tcgccgaagc cacgcagctc ctctccgacg gggacctgaa ccaccgggtg gacgtcgaga
     1801 gcaaagacga ggtcggggcc ctgtcggccg cattcaatca catggccgag acgctggaag
     1861 ggcagcgtca ggagatcgtg aaccggcagc aggagctgga gcgactgagc agcgagctaa
     1921 aggccatcaa cacgaactac gtcgagatgc tgggcttcgt gacccacgag ctgaagaacc
     1981 ccctggcctc cgccatgatg agtctgtata cggtgaagga cggctacctg ggggagctga
     2041 acccggccca gctgaagagc ctgggctcgg tggctcagag cctggactac ttccacgaca
     2101 tggtcaagaa ctacctggat ctctcccggc tggagaaggg ggagttggag gtggcgaagc
     2161 ggcaggtccc actgcacgcc cgggtggtcc ggccggtact cgatgcgctg gagcgggaga
     2221 tgcgggagcg gcgtttcgtc ctccaggacc acatcccgga cgatgcgatc ctgaccacgg
     2281 acgaaaacct gctccggatc gtctacgaca acctgctctc caacgccctc aagtatggcc
     2341 gcgagggcgg gaccattcaa ctggactact tcgcgaacca ctcccaggtg gtcctcggcg
     2401 tcaaaaacga tggcgcgggc atccccgccg agaagctgcc gctcctcttc gaaaaattca
     2461 gccgactgga tacgccggag cactccatca agaaaggctc cgggctgggg ctgtacatct
     2521 gcagggagat cgtgcggaag atgggcggcg agatctgggt ggattcgagg cccggggaat
     2581 gggtcacgtt cagcttcagc cttcccaagt aacccgagga ggacatcccg atggcgaaga
     2641 agatcctggt gatcgacgac gatgtgcagc tcgtggatac ggtgaagacc ctgctgcaga
     2701 gcgtggggta cgaggtcgcc tcggcgtatc aggccgaaaa gggagtggag ctggcgcgcc
     2761 agatcacgcc cgatctgatc ctgctggacg tgatgttcgc cggccccccg gggccggacg
     2821 gtatcgagat gtcgcggcgg atccagcagg ataccgcgct gaaggacatc ccggtaatca
     2881 tcctctccgg cgtccggaaa gtcctggacc tcccgttcaa gatcgggccg gatgagacct
     2941 ggatgccggt gaagacgttc ctggagaagc cgatcaagcc ggatcggctg ctgc
//
LOCUS       DBNGZC010000304         4657 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1395033, whole genome shotgun sequence.
ACCESSION   DBNGZC010000304 DBNGZC010000000
VERSION     DBNGZC010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4657)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4657)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4657
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1395033"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="AC16G0_05555"
     CDS             <1..390
                     /locus_tag="AC16G0_05555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020135.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR5099124.1"
                     /translation="LYRDLATLFVELSREVAQVAKCLNIPLDDFRGFEVKTVCTGPLE
                     EAVASVMRRGKVMQERGMTGVKISTLQDLERGKRTEVEEVIGYVVRLAEEHGVAVPKL
                     QLLYRIIRGMEAARQEASVPASTQVRG"
     gene            401..3853
                     /gene="metH"
                     /locus_tag="AC16G0_05560"
     CDS             401..3853
                     /gene="metH"
                     /locus_tag="AC16G0_05560"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004946263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HIR5099125.1"
                     /translation="MASDFRQRLRERVLIFDGAMGTSIQDRNLAPEDFGGKDGCNEVL
                     VLTRPQVIREIHAGFLGVGCDAIETDTFGASRLVLGEYDLADKAYEINRAAARLAREV
                     TADFSTPSHPRFVVGSLGPGTRLPSLGQIGFDELVSMYTEQARGLLDGGVDVLLIETC
                     QDLLQAKGAIVGCRDAMQGFGKDVPLMVQVTMESTGTMLLGTEIGAALTALEPYGLDV
                     IGMNCATGPQEMMEHIRFLSRHSRPAISCLPNAGLPKMEGGRARYLLTPDELAGFHQT
                     FVEEHGVSIVGGCCGTTPAHLKRVVEVIGQRAPGLRRPEYVPASSSLYVSTPFRQDQS
                     FLIVGERTNANGSKQFREHLLAGDYDGMVGMAREQAREGAHVLDVCVDYVGRNGQTDV
                     DEIVRRFVTQCTLPLVIDSTEAPVIEAALKRIGGRSVINSVNLEDGEQGRPARIFPMA
                     KRYGAAVVCLLIDEEGQARTLEWTLRVAHRLYDLAVKDYGLEPADLIFDALTFPLGSG
                     QADLRRDAIATLDAIRRIKAELPGVFTILGVSNVSFGLKPAARQVLNSVFLHQAIEAG
                     LDAAIVNVSRILPLNRIDPRQLEVARHLIFDERRDGYDPLTEFMGLFDKASGGKTAVV
                     REAPKTLEERLKARIVDGERPGLEALLDEGLGKYDALTLINDILLDGMKTVGDLFGSG
                     QMQLPFVLQSAEVMKAAVGYLERFMSRADGQSKGKIVLATVKGDVHDIGKNLVDIILS
                     NNGYTVVNLGIKQPIHNIIEAALAHQADAIGMSGLLVKSTLVMKENLEELNARDLSRF
                     PVILGGAALTRGYVEGDLRALYKGDVFYAGDAFDGLHLMAEIASRETAEAAVVRPMLR
                     RAPPRREPSPAPIPATSGERIRSDVRREVPIPAAPFLGSRVVTEIPLADVFAFVNEVA
                     LFRTQWQFHRGKGDREHFDELIEGHVRPIYERCKARCIDELILEPRAVYGYFACQSEG
                     DDLIVYAEDGRTPRVRFTFPRQPAGRRLCLADFFAGVDSGRMDVLGCMLVTVGRRASE
                     FTKELFEQHHYTDYLYFHGLSVESAEALAELWHKRMRDELGIAGEDSPDLARIFRQGY
                     RGSRYSFGYPACPNLEDQAKLFELLEPERIGVELSQEFQLDPEQSTSAIIVHHPEAKY
                     FNIA"
     gene            4235..>4657
                     /locus_tag="AC16G0_05565"
     CDS             4235..>4657
                     /locus_tag="AC16G0_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099126.1"
                     /translation="MSKRKVTRREFLELATLTAAGVALPNSVVGAAQAGAAAAKPAQA
                     GEKLGQRLIGKLEGPEIIRDVTKFPKTFKEAPMLAELVKAGKLPPVDKRLPDPSDVMV
                     IKPLKEVGKYGGRWRRGFTGPADNENGNRIVSTDKLLMF"
BASE COUNT          835 a         1430 c         1595 g          797 t
ORIGIN      
        1 ctctaccgag atctggccac cctgtttgtg gagctgagcc gggaggtggc ccaagtggcg
       61 aaatgtctga atatccccct ggacgatttc cggggcttcg aggtgaagac ggtgtgcacc
      121 gggcctcttg aagaggcggt ggcctccgtg atgcggcgcg ggaaggtcat gcaggagcgg
      181 gggatgaccg gggtgaagat ctccacgctc caggatctgg aacgagggaa gcgcacggag
      241 gtggaggagg tcatcggata cgtggtgcgc ctggcggagg agcacggcgt ggccgtcccc
      301 aagctccagc tcttgtaccg gatcatccga gggatggagg cggcccggca ggaggcttcg
      361 gtgccggcct ccacgcaagt cagaggatag gcgagggaca atggcgtcgg attttcggca
      421 gcgtctcagg gaaagggtcc tcatctttga tggggccatg ggcacatcca tccaggaccg
      481 gaacctcgcg ccggaagact tcgggggaaa ggatgggtgc aacgaggtcc tcgtcctgac
      541 cagaccgcag gtgatccgcg agattcatgc gggctttctg ggggtcggct gcgacgccat
      601 cgagaccgac actttcggtg ccagcagact cgtcctgggg gaatatgacc tggcggacaa
      661 ggcgtacgag atcaatcgcg cggcggccag actcgcgcgc gaggtgacgg ccgacttctc
      721 cacgccgtcc cacccgcggt tcgtcgtggg ctccctcggg ccaggcacgc gcctgccgtc
      781 gcttggacag atcggcttcg acgaactggt ctccatgtac accgagcagg cgcggggtct
      841 cttggacggt ggggtggacg tcctcttgat cgagacctgc caggatctcc tccaggccaa
      901 gggggcgatc gtgggatgcc gggatgcgat gcagggcttt gggaaagatg tccccctcat
      961 ggtccaggtg acgatggaga gcaccgggac gatgctcttg ggcacggaga tcggggcggc
     1021 gctgacggcc ctggagccct acggtctgga cgtgatcggc atgaactgcg ccaccggtcc
     1081 ccaggagatg atggagcaca tccgcttcct gagccggcac tcccgcccgg ccatctcctg
     1141 cctgccgaac gccgggctgc cgaagatgga aggaggccgg gcacgctatc tcctcacgcc
     1201 cgacgagctg gccgggttcc accagacctt tgtggaggag cacggcgtct ccatcgtggg
     1261 cggctgctgc ggcaccacgc cggcgcacct gaagcgggtg gtggaggtga tcggccagcg
     1321 cgcccccggc ctccggaggc cggaatacgt gccggcatcc tccagcctgt acgtgagcac
     1381 cccgttccgg caggaccaga gcttcctcat cgtcggagag cggacgaacg ccaacggctc
     1441 caagcagttc cgtgagcatc tcctggccgg agactacgac ggcatggtgg ggatggcgcg
     1501 ggagcaggcc agggaggggg cgcacgtcct ggacgtgtgc gtggactacg tcggccgcaa
     1561 tgggcagacg gacgtggacg agatcgtccg gcggttcgtc acccagtgca cgctccccct
     1621 ggtcattgat tccaccgagg cgccggtgat cgaagcggcg ttgaaacgga tcggcggccg
     1681 ctcggtgatc aactcggtca acctggagga cggggaacag ggccgcccgg cccggatctt
     1741 ccccatggcc aagcgctacg gggcggcggt ggtgtgcctc ctcatcgacg aggagggaca
     1801 ggcgcgcacc ctggaatgga cgctccgggt cgcgcatcgg ctgtatgacc tggcggtcaa
     1861 agactatggg ctggagcctg ccgacctgat cttcgacgcc ctgaccttcc ccctgggttc
     1921 ggggcaggcg gacctgcggc gggacgccat cgccacgctg gatgccattc gccggatcaa
     1981 ggccgagctg ccgggagtct tcaccatcct gggcgtgagc aatgtttcct tcggcttgaa
     2041 gccggcggca cggcaggtcc tcaacagcgt gttcctgcat caggccatcg aggccggtct
     2101 ggacgcggcc atcgtgaacg tgtcgcggat cctgcccctg aatcggatcg atcccaggca
     2161 actggaggtt gcgcgtcatc tcatcttcga cgaacggcgc gacggctatg atcccctcac
     2221 cgaattcatg ggtctcttcg acaaagccag cggcggcaag accgcggtgg tcagggaggc
     2281 gcccaagacg ttggaggaac gcctcaaggc ccggatcgtg gacggcgagc ggccgggtct
     2341 ggaggctctc ctggatgagg ggctcggcaa gtacgacgcc ctgaccctca tcaacgacat
     2401 cctgctggac ggcatgaaga ccgtgggcga cctgttcggc agcgggcaga tgcaactgcc
     2461 cttcgtcctc cagtcggcgg aggtgatgaa ggccgccgtg gggtacctgg agcggttcat
     2521 gtccagggcc gacgggcaga gcaagggaaa gatcgtcctg gcgacggtca agggggatgt
     2581 ccacgacatc ggcaagaacc tggtggatat cattctcagc aacaacggct acacggtggt
     2641 gaacctcggc atcaagcagc ccatccataa catcatcgag gcggcgctgg cgcatcaggc
     2701 agatgccatc ggcatgagtg gcctcctggt caagtccacc ctggtgatga aggagaacct
     2761 ggaggagctg aacgcccgag acctgtcacg ctttccggtg atcctgggcg gggccgccct
     2821 gacgcggggg tacgtggagg gggatctgcg ggctctctac aagggagacg tgttctacgc
     2881 gggagacgcc ttcgacggcc tgcatctgat ggcagagatc gcctcgcgtg aaacggcgga
     2941 ggcagccgtc gtccggccca tgctgcggcg ggcaccccct cgccgcgagc cgtcacccgc
     3001 ccccatcccg gctaccagcg gggagcggat ccggagcgat gtccggcggg aagtcccgat
     3061 cccggcggcg cctttcctcg ggagcagggt ggtgaccgag atcccgctgg cggacgtctt
     3121 cgccttcgtc aacgaggtgg cgttgttccg cacccagtgg cagttccacc gggggaaggg
     3181 ggaccgggag cacttcgacg agctaatcga gggccacgtc cggccgatct acgagcgctg
     3241 caaggcccgc tgcatcgacg agctaatctt ggagccgcgg gcggtctatg gctacttcgc
     3301 atgtcaatcg gagggcgacg acctgatcgt gtatgccgag gatgggcgga ccccccgtgt
     3361 gcggttcacg ttcccgcgcc agcctgccgg gcggcgcctc tgtctcgccg acttcttcgc
     3421 cggggtggat tcggggcgga tggatgtcct cggctgcatg ctggtcaccg tggggcgccg
     3481 agcctccgag ttcacgaagg aactgttcga gcagcaccac tacaccgact atctctattt
     3541 tcacggcctc tcggtcgaat cggcggaagc cctggcagag ctgtggcaca agcggatgcg
     3601 ggacgagctt ggaattgcag gggaggactc tccggacctt gcccggatct tccggcaggg
     3661 ctaccggggg tcgcgctaca gcttcggcta tccggcgtgc cccaacctcg aggatcaggc
     3721 caagctcttc gagctactgg agcccgaacg gatcggggtg gaattgagcc aggagttcca
     3781 gctcgacccg gagcagtcca cctcggcgat catcgttcac caccccgagg ccaagtactt
     3841 caacatcgcc tgagacacct gaaccgcagc cacagatttc gcagattacg ccgattattt
     3901 ctgaaggttt ccatccgtga aatctgcgca atctgcggtt agaggaagtc ccgggtgttc
     3961 tcgaagagaa acgtgattct cttcgccatg atggccgcgg cccctgacga gtcgggggtg
     4021 agggtcctat acagcgtaac gccaaccatt tcccgttgac accccggtca cccttcgata
     4081 ggctctcgcc cgtgcctccc ggaacctgac tcacgcttgc gggagactgt cccttctcca
     4141 tgatccactg cgtagaatgg gccgtgttgg ccaggtgggt aaggcctgtg gggcgttcga
     4201 cttaatccgt ttcgcagcaa agggggtagt actcatgtca aagcggaagg tgaccaggcg
     4261 cgagtttctg gagttggcaa ccttgacggc agccggtgtt gctcttccca actctgtcgt
     4321 cggggcggcc caggcggggg cagcggcagc caaacctgcc caggccgggg agaagcttgg
     4381 ccaacggctg atcgggaaac tggaagggcc ggagatcatt cgcgacgtca cgaaattccc
     4441 caagaccttc aaggaagccc ccatgctggc ggagctggtc aaggccggca agctgcctcc
     4501 cgtcgacaaa cgcctgcctg atccgagcga cgtgatggtc atcaagccct tgaaggaggt
     4561 cggaaagtat ggcggccggt ggcgccgcgg cttcaccgga cccgccgaca acgagaacgg
     4621 caaccgcatc gtctccaccg acaagctcct gatgttc
//
LOCUS       DBNGZC010000305         1813 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1981616, whole genome shotgun sequence.
ACCESSION   DBNGZC010000305 DBNGZC010000000
VERSION     DBNGZC010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1981616"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..172
                     /locus_tag="AC16G0_05570"
     CDS             <1..172
                     /locus_tag="AC16G0_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5099127.1"
                     /translation="SVPGAILGGIFLGVVEVFAAYYISSAYRDAFAFILLILVLLFRP
                     QGFFGEKIAEKA"
     gene            182..>1813
                     /locus_tag="AC16G0_05575"
     CDS             182..>1813
                     /locus_tag="AC16G0_05575"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HIR5099128.1"
                     /translation="MNPRQAKPLLLVVLALAVLVFPWMVSSSYWLNLVNLAIGFSVAC
                     LGLNIVLGYTGQLSLAQAAFWGVGAYTSALLTTQLGVPVWGGMIAAFVIAGFFGVLLG
                     IPTLKLTGHYLAMTTIGFGIILQLILINAIWLTGGSDGIPKIPSPGIGSYELKDPNTF
                     FYVAAVSLILLTWASMRLKDSRVGRAFMAIRENEMAAETTGVDSTYYKIVAFALSAAY
                     GGFGGWMFAHSGSHYISPDTFSFDQSVVLLAMAVLGGNGSAVGSVVGAILLTLLPEVL
                     RFLKDSYMMVYAAGIVAVMIFMPGGIAGLVWTFPLSQRLRAWWATGSAAARQVAADGK
                     DPKGARATAGPQPGKGAGVKGNGTLLTVKSLAKHFGGLKAVDGVDMQVRRGEIQALIG
                     PNGSGKTTILNMLSGLYVPTAGEITLDGTPITGPRPHTITAHGVARTFQNIRLFGELS
                     VLENVLIGQHSRSRAGLLASIVRPASQRSEEAVLREKALEALAFVGLRGKEFSEARSL
                     PYGQQRLLELARALVSEPKLLLLDEPAAGLNAAETE"
BASE COUNT          268 a          615 c          591 g          339 t
ORIGIN      
        1 gagcgtcccc ggcgccatct tgggtggcat cttcctgggc gtggtcgagg tcttcgccgc
       61 ctactacatc tccagcgcct accgcgacgc cttcgccttc atcctcctga tcctggtgct
      121 gctcttccgc ccgcaagggt tcttcggcga gaagatcgcc gagaaggcat agggggcggc
      181 catgaatcca cgtcaggcca agccgcttct ccttgtggtc ctcgccctcg cggtgctcgt
      241 cttcccgtgg atggtgagca gcagctactg gctcaatctg gtgaacctgg ccatcggctt
      301 ttccgtggcc tgcctggggc taaacattgt cctggggtac acgggacagc tctccctggc
      361 ccaggcggcc ttctgggggg tgggcgccta tacgtcggcc ctcctcacga cccagctggg
      421 ggtgcccgtc tgggggggca tgatcgccgc cttcgtcatc gcgggcttct tcggggtcct
      481 cctggggatc cccaccctga agctcaccgg ccactacctg gccatgacca ccatcggctt
      541 cgggatcatc ctgcagctca tcctgatcaa cgccatctgg ctcaccggcg ggtcggacgg
      601 cattcccaag atcccctcgc caggcatcgg ttcctacgag ctgaaggacc ccaacacctt
      661 cttctacgtc gccgccgtct cgctaatcct cctgacctgg gcatccatgc ggttgaagga
      721 ttctcgtgtc ggccgcgcct tcatggccat ccgcgagaat gagatggccg cagagaccac
      781 cggggtggac tcgacctatt acaagatcgt ggcctttgcc ctctcggcgg cctacggggg
      841 tttcggggga tggatgtttg cccacagcgg ctcgcactac atcagcccgg ataccttctc
      901 cttcgaccag tcggtggtcc tcctcgccat ggccgtgctg ggtgggaacg gctccgccgt
      961 cggttccgtg gtgggtgcca tcctcctgac gttgctgccc gaggtcctgc gcttcctcaa
     1021 ggattcctac atgatggtct atgcggccgg catcgtggcc gtcatgatct tcatgccggg
     1081 cgggatcgcc ggactggtat ggacctttcc gctcagccag cggcttcgag cctggtgggc
     1141 caccggctcg gcggctgcgc gccaggtggc ggcggatgga aaggatccga agggcgcccg
     1201 tgccacggcc ggcccccagc cggggaaagg ggccggcgtc aagggcaacg gcaccctctt
     1261 gaccgtcaag agcctggcca agcatttcgg cggcctgaaa gcggtggacg gcgtggacat
     1321 gcaggtccgg cggggcgaga tccaggcgct catcgggccc aacggctcgg gcaagaccac
     1381 catcctgaac atgctgagtg gcctgtacgt ccccacggct ggcgagatca cgctggatgg
     1441 aacgccaatc accggcccga ggccccacac catcacggcc cacggggtcg cccggacctt
     1501 ccagaatatc cgcctgttcg gcgagctctc ggtgctggag aacgtgctga tcggacagca
     1561 cagccgctcc cgggcggggc tgctggccag catcgtccgg ccggcctcgc agcggtcgga
     1621 agaggccgtc ctgcgggaga aggccctgga ggccctggcc ttcgtggggc tgcgaggcaa
     1681 ggagttctcc gaggccagga gtcttcccta cgggcagcag cggctcctgg agctggcgcg
     1741 agccctggtc tcagagccca agctgcttct gctggatgag ccggcggccg gcctgaacgc
     1801 ggcggagacc gag
//
LOCUS       DBNGZC010000306         1184 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_807792, whole genome shotgun sequence.
ACCESSION   DBNGZC010000306 DBNGZC010000000
VERSION     DBNGZC010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_807792"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="AC16G0_05580"
     CDS             complement(<1..843)
                     /locus_tag="AC16G0_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099129.1"
                     /translation="MLAVIGHVAKKEFRQIQRDRRSLALILIMPIVLTVLFSKALETG
                     ELRNIPLAILNLDGSPESNVIVTSLSTHAEIQIQQQAATLGEAQALLARGKIKAAIIF
                     PKGFMREIESGGEAQLQLLLDGTDNVSAPLVEGVAQRVIQRYNTERALKGLWARGVRP
                     DRGLRLVRPVYVQTEIRYNPGLQFLSYAMPGVIGLTLQLLTVMLMAVSVTRERERGTL
                     DQLMASPISRVELILGKVLPYFGISLVNVATILFVADRWFNVPVGKNLPLLLTLCGLF
                     VLSSL"
BASE COUNT          213 a          386 c          387 g          198 t
ORIGIN      
        1 caaggacgac agcacgaaga gcccgcacag agtgagcagg agcggcaggt tcttccccac
       61 tggcacgttg aaccaacgat cggcgacgaa gaggatggtg gccacgttga ccagcgagat
      121 gccgaagtag ggcaggacct tccccaggat cagctcgacg cggctgatgg gtgacgccat
      181 gagctggtcc agggtgcctc gctcccgctc gcgtgtgacg ctgacggcca tgagcatcac
      241 ggtcaggagt tgcagcgtga ggccgatgac gcccggcatg gcgtaactca gaaactgcag
      301 gccgggattg taccgaattt cggtttgcac gtagacgggc cggacgagcc ggagcccgcg
      361 gtcgggccgc actccgcggg cccacaaccc cttgagcgct cgctcggtgt tgtagcgctg
      421 gatgacgcgc tgcgccacgc cctctaccag gggcgccgag acgttgtccg tcccatccag
      481 gaggagctgg agctgcgcct ccccgcctga ctcgatctcg cgcatgaacc ccttgggaaa
      541 gatgatggcc gccttgatct tcccgcgggc caagagagcc tgggcctctc ccagcgtcgc
      601 cgcctgctgc tggatctgga tctcggcatg ggtgctgagg gatgtgacga tgacattgga
      661 ttccggtgat ccgtccaggt tgaggatcgc caagggaatg ttccggagtt cacctgtctc
      721 cagcgccttg ctgaagagga ccgtgaggac aatcggcatg atcaggatga gggccaggct
      781 ccgccgatcg cgctggatct ggcggaactc cttcttggcc acgtggccga tgactgccag
      841 catgctcaac acctcgaaac gtcaaacggc gtccgacggg tcagtctccg acagaccgca
      901 tcgcacatcg acgcacgggc cggagtcccc gcacggggaa tcgggcctgg gtgggggcaa
      961 gctgtgcccg cgggcatggg tctctcgccc ctggcctgaa gacgtgagcc ctttgcgcgg
     1021 aggtactcac cccggcaacc ccgtcgaggc cccgccaccc ctcgaccacc cgcatgacgg
     1081 ggacggcatt catccccagg gcggcttccc cccaggtccg gcgcagtgta ttcggacccc
     1141 cctccgccgt tcagatgcaa gttattgcgg atttcccagg aacg
//
LOCUS       DBNGZC010000307         2454 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1099920, whole genome shotgun sequence.
ACCESSION   DBNGZC010000307 DBNGZC010000000
VERSION     DBNGZC010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1099920"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(275..1645)
                     /locus_tag="AC16G0_05585"
     CDS             complement(275..1645)
                     /locus_tag="AC16G0_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099130.1"
                     /translation="MKHYGRAVVGLSLLLVGLLGAVVFATGFTPAVGKENKNFDKVIR
                     QNSDDMVKDGRQTFRFDTFGDEAFWGDTLHLHEAIQGAKFGGVGPGVSPRTALAVGLK
                     VDLDALPLSVVQALKAGQVNLDDPAVTLTLLKLNAVLGVTGFFDPAGHLQSMGIQCAL
                     CHSTVDDALAPGIGHRLDGWANRDLNVGAIVSLAPNLQPVADLLSVDVPTVKAVLASW
                     GPGKFDAELFFDGKAVNPEQVSNGVVTGTNVPAATLLPPAFGLAGVNLHTYTGWGSVT
                     YWNAFVANLEMHGKGTFFDPRLNDATQFPIAAREGFGNVQSDHDLITPKLAALHFYQL
                     AIPAPEPPKGSFDEAAAARGKVLFEGQAKCAMCHVPPLYTEPGWNMHTPEEIGIDAFQ
                     ANRAPDKRYRTTPLKGLWTHMQGGFYHDGRFATLNDVIAHYDQTFGLRLTSQEVHDLV
                     EFLKSI"
     gene            complement(2047..2131)
                     /locus_tag="AC16G0_05590"
     tRNA            complement(2047..2131)
                     /locus_tag="AC16G0_05590"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2095..2097),aa:Leu,seq:gag)
BASE COUNT          447 a          743 c          812 g          452 t
ORIGIN      
        1 gagggaagtc gggcggagct ccatggccgt gggcaggccc gagcatgcga tctggcgatt
       61 cctgtctcaa gtcctgcagg gccccctcag acaatcccga gggacgccag cctcggtggc
      121 cgagaaggcg gtgacctgct ccgcgagcag gctccagccg ctcaaagggt ggacacaagc
      181 ccggcgaccg aatccggtcg ccgggcttgc tggtgctata gcctcccgct ggtacatccg
      241 tcatgcctcg aatgcgttat tcgaggctct cgacctagat tgacttcaag aactcgacca
      301 gatcgtgaac ctcctgggag gttaacctca ggccgaacgt ctgatcgtag tgggcgatca
      361 catcgttcaa cgtggcgaag cgcccgtcgt ggtagaaacc gccctgcatg tgggtccaga
      421 ggcccttcag cggggtggtc cggtagcgct tgtcgggggc ccgattcgcc tggaaagcgt
      481 ctatcccgat ctcctccggc gtgtgcatgt tccagcccgg ctccgtgtag agcggcggca
      541 cgtggcacat ggcgcacttc gcctgcccct cgaaaaggac ctttccccgc gcggccgccg
      601 cttcgtcgaa gctccccttg ggaggctcgg gcgccgggat ggcgagctgg tagaagtgaa
      661 gcgcggcgag cttcggggtg atgagatcgt gatcgctttg cacgttgcca aacccctcgc
      721 gggcggcgat gggaaattgc gtggcgtcat tcaatcgtgg atcgaagaac gttcccttgc
      781 catgcatctc cagattcgcc acgaaggcat tccagtaggt caccgacccc caccccgtat
      841 atgtgtggag gttgactccg gcgaggccga aggccggcgg gagtagtgtg gcggccggca
      901 cattcgtccc ggtcacgacg ccgttactca cctgctcggg attgacagct ttcccgtcga
      961 agaacagctc cgcgtcaaac ttgcccggtc cccaactggc caggaccgcc ttcaccgtgg
     1021 ggacgtccac actcaggagg tccgcgacgg gctggaggtt tggggcgagc gacacgatgg
     1081 ccccgacatt gaggtcgcgg ttggcccacc catccaggcg gtggccgatc ccaggggcca
     1141 gcgcgtcgtc cacggtggag tggcagaggg cgcactggat ccccatggac tgcaaatgcc
     1201 cggccgggtc gaagaagccg gtgacgccca agacggcgtt cagcttcagc agggtgaggg
     1261 tgacagccgg atcgtccagg ttcacctggc cggctttcag ggcttgcacg acgctcagcg
     1321 ggagggcgtc gaggtcgacc ttcagaccca ccgccaaggc ggttcttggg cttacgcccg
     1381 gaccgacgcc gccaaacttc gcgccttgga tggcctcatg aaggtgcaag gtatcacccc
     1441 agaatgcctc gtcaccaaag gtgtcgaagc ggaaggtctg ccgaccatct ttgaccatgt
     1501 catcggagtt ctggcggatg accttgtcga agttcttgtt ctccttgcct accgcaggcg
     1561 tgaagcccgt ggcgaagacc acggcgccca ggaggcctac gaggagaaga gagagcccga
     1621 cgacggctcg accgtaatgt ttcatgatag acctccttca cctgatgacg gttccagttt
     1681 gtacgcctct tgcccggcgg gttgcctctc gcccgaaccc ctgcaatccc gccgggcata
     1741 aaacgcacgc cgctcgcaac tgacgggtcg gttaggctcg tttgtggggg tgcggtagtg
     1801 gtccaggctg tcttgaggca ggaacccctc cgcgccactc gtggggtatc gacttctgcc
     1861 ccatttgagg gcatgagccg attgaatctt acgttgcgct tctcaacttt ctggatgggt
     1921 tgcctgacag cggatggatt gaggggggtt gagtgtgagg gcgagtgggt ttcgagtacc
     1981 caactgggcg agcggaaaac acgaaaccgg cgtcccgatc gggtcgccgg ttttgtgagg
     2041 cgaggatggt gccgaagggg ggactcgaac ccccacgggt tgccccacac gcccctcaaa
     2101 cgtgcgcgtc tgccagttcc gccacttcgg ctcggcgcaa tatctaccgc ctcacgcggc
     2161 ggattgtcaa tggaaaagag ccgaagttat tcgggccgga gcgagagagg tacgtccggt
     2221 cacgacaaga cggaaggctg gcggcgaaaa cctgaacgcg caacacaaag atgcggcctg
     2281 cgggatcggc ttgaggtccc tgcttgtatc cggtatcatg gccgcaacct cgaaggcaaa
     2341 ggagaccacc gaatgcgcgg agcgagaggg atggcagtcg cgggtgtcct ggtcttgctg
     2401 ttggccgggt gcgccacggt tgagatccag cagacccggg tcgtgacggg ccag
//
LOCUS       DBNGZC010000308         3075 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_123245, whole genome shotgun sequence.
ACCESSION   DBNGZC010000308 DBNGZC010000000
VERSION     DBNGZC010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_123245"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..608
                     /locus_tag="AC16G0_05595"
     CDS             141..608
                     /locus_tag="AC16G0_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014354877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5099131.1"
                     /translation="MPAKFVHTNLIAQNWKGLVAFYEAVFGCTRLPPERDLSGDWLDA
                     VTGIPHARIRGVHLRLPGYAGAGPTLEIFQYDREEERPATAVNRPGFGHIAFAVEDVG
                     AAREAVLAAGGHAVGDLVSTKIPGAGTISVVYVTDPEGNVIELQQWADETEAT"
     gene            605..808
                     /locus_tag="AC16G0_05600"
     CDS             605..808
                     /locus_tag="AC16G0_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099132.1"
                     /translation="MSKGGIGPVRKALAAMCHACPLCNFGREHPGSLLGRVLNHRLHA
                     DHCPMWKAEKALYAPSRKEPTAA"
     gene            893..2308
                     /locus_tag="AC16G0_05605"
     CDS             893..2308
                     /locus_tag="AC16G0_05605"
                     /EC_number="4.1.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050371 - tyrosine phenol-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HIR5099133.1"
                     /translation="MSQPTFRTMGQQFGRRSWAEPWKIKMVEPIRIPSREERERALVE
                     AGYNTFLLRSEDVYIDLLTDSGTGAMSDRQWAGIMLGDEVYAGSRNFYHLEDAVRTTY
                     GYRHLVPTHQGRGAEHLISRALITPGQYVPGNMYFTTTRLHQELAGGTFVDVIIDEAH
                     DPASLHPFKGNVNLDKLEALIKRVGAEKIAYVSLAGTVNMAGGQPVSMANVRALRALC
                     DRYGVRIYLDATRMVENAYFIQEQEAGYASTPIAEILKEFCGQTDGAWMSAKKDNLVN
                     IGGWLAVNDDDLFDRLRNMVVIYEGLHTYGGMAGRDMEALAIGIAESVQDDHVRARVG
                     QVRYLGDLLLAWQIPIVQPVGGHAIFLDAKRFYPHIPQDQFPAQVLTAALYLDSGVRG
                     MERGIVSAGRDPQTGDHRRPALELTRLAIPRRVYTQAHMDVVAEAVKAAYDAREQARG
                     LKMVYEPKYLRFFQARFERLT"
     gene            2399..>3075
                     /locus_tag="AC16G0_05610"
     CDS             2399..>3075
                     /locus_tag="AC16G0_05610"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR5099134.1"
                     /translation="MKDLLTQVAERATRSLESLGELGVAPTSAAIQRLSELGGPLPGA
                     PTDPETVLRLLDEIGSPATVASAGGRFFGFVIGGSLPATLAANWLAGAWDQNAGLIVA
                     SPVAAALEEIALQWLRDVLALPPQSAGAFVTGATMANFTALAAARHAVLEAAGWNVEA
                     EGLFGAPPITVIVGEEAHPSLLKSLGLLGLGRKRVVTVPVDGQGRMRPEAFPRIAGPT
                     IVCIQAG"
BASE COUNT          541 a          976 c         1049 g          509 t
ORIGIN      
        1 cggaccaagg gaatcatcca gaacgtcgcc atcctcggcg ggcaaagtct gggcttacac
       61 ctcagggaat ccgagcggtg gtgatccgat gaggctacga gagactcccc gtgccgctga
      121 gtccgaggtg agatgtgtcg attcctgcca agtttgtcca tacgaatctg atcgctcaga
      181 actggaaagg cctggttgct ttttacgagg cagtgtttgg ctgcacgcgg ctgccaccgg
      241 agcgggacct gtccggggac tggctggacg ccgtcacggg cattccacat gcccggatcc
      301 gaggagtcca tctacgcctg ccgggatatg cgggggccgg tccgacgctt gagatcttcc
      361 agtacgaccg ggaagaggag cggcccgcga ccgccgtcaa tcgtccgggg tttggccaca
      421 tcgccttcgc cgtagaagat gtcggcgcag cccgggaagc cgtgctcgcg gccggcgggc
      481 atgcggtggg ggacctcgtg tcgacgaaga ttccaggcgc aggcacgatc agcgtcgtct
      541 atgtgacgga ccccgagggc aacgtgatcg agctacagca gtgggcggac gaaacggagg
      601 cgacatgagc aaaggcggta tcggccctgt gaggaaagcg ctggcggcga tgtgtcatgc
      661 ctgccccctc tgcaattttg gccgggaaca tcccggatca ctcttgggca gggtgctgaa
      721 ccatcgactt catgccgatc actgccccat gtggaaggcg gagaaagcgc tgtacgcccc
      781 cagccggaag gaaccgacgg cggcctgacc ttgcctcggc ccaggccggg ggctcgcgcg
      841 gagtgtgccc gggggtattc tcacaggatg catccatagg cggaggagag acatgtccca
      901 gccaaccttc cggaccatgg gtcagcaatt cggtcgccga tcctgggcgg agccctggaa
      961 gatcaagatg gtggagccga tccgcatccc cagccgggag gagcgggagc gggcgctcgt
     1021 cgaggcgggt tacaacacct tcctgctgcg ctccgaggac gtgtacatcg acctgctgac
     1081 cgacagcggc acaggggcca tgagcgaccg ccaatgggcg ggcatcatgc tgggcgacga
     1141 agtctacgcc ggcagccgca acttctacca cctggaggac gccgtccgga cgacctacgg
     1201 ctatcgtcac ctggtgccga cgcaccaggg tcgaggggcg gagcacctga tcagtcgcgc
     1261 gctgatcacg cccggccagt acgtccccgg caacatgtac ttcaccacca cgcgactgca
     1321 ccaggagctc gccgggggga cctttgtgga tgtgatcatc gacgaggccc acgaccccgc
     1381 gagcctccat cccttcaagg ggaatgtgaa cctggacaag ctggaggcgc tgatcaagcg
     1441 ggttggtgcc gagaagatcg cctacgtcag tctggcggga accgtgaaca tggccggagg
     1501 gcagccggtc agcatggcca acgtgcgggc gctccgcgcc ctgtgcgatc gctacggcgt
     1561 ccgcatctac ctggacgcca cgcggatggt cgagaacgct tacttcatcc aggagcaaga
     1621 agcggggtac gcaagcacac cgatcgccga aatcctgaaa gagttctgcg ggcagaccga
     1681 cggcgcctgg atgagcgcca agaaagacaa cctggtcaac atcggcgggt ggctggcggt
     1741 caacgacgac gacctcttcg atcggctccg caacatggtc gtgatctacg agggcctcca
     1801 cacctacgga gggatggccg gacgcgacat ggaagccctg gccatcggca tcgccgagtc
     1861 ggtccaggat gatcatgtcc gcgcccgggt gggccaggtg cgctacctgg gtgatctcct
     1921 gctcgcctgg cagatcccca tcgtccagcc cgtgggcggc cacgccatct tcctggatgc
     1981 caagcggttc tatccgcaca tcccccagga ccagttcccg gcccaggtcc tcacggccgc
     2041 cctttacctg gattcgggcg tgcggggcat ggagcgcggt atcgtcagcg ccggacggga
     2101 tccccagacg ggcgatcacc gccgtcctgc gctggagtta acgcgcctgg cgattcctcg
     2161 gcgcgtctac acccaggcac atatggatgt cgtcgccgaa gccgtcaagg cggcctacga
     2221 tgctcgcgag caggcgcgag gtctgaagat ggtgtacgag cccaagtacc tgcggttctt
     2281 ccaagcccgg ttcgaacgct tgacttgagg gggcgggagc ctgcgcgtgc aggcgcgctt
     2341 cacttcacgg caactggcgc cccatggcga gaccgatggc ggcagagagg atgacgcgat
     2401 gaaggatctg ctgacacagg tggcggagcg agccacgcgg tctctcgaga gtctggggga
     2461 actgggcgtt gcaccgacat cggccgccat ccagcgtctt tcggaattgg gcgggccgct
     2521 tccgggagcg ccaaccgacc cggagaccgt ccttcgtctg ctggacgaga tcggttctcc
     2581 tgccacggtt gccagcgcgg gcgggcgctt cttcgggttc gtgatcgggg gttcgcttcc
     2641 ggcaaccctg gcggcaaact ggcttgccgg ggcgtgggat cagaacgccg gtctcatcgt
     2701 cgcctctccg gtggctgcgg ccctggagga gatcgccctc cagtggctgc gcgatgtgct
     2761 ggcattgccc ccgcagtctg ccggcgcgtt cgtgactggc gccaccatgg ccaattttac
     2821 ggccctggct gccgcgcgcc acgccgtcct ggaagcagcc ggatggaatg tcgaggccga
     2881 gggtctgttc ggcgcgccgc cgatcacggt cattgtcggt gaggaggccc acccgagtct
     2941 cttgaagtcc ctgggcctgc tggggctggg acgcaagcgc gtggtcacgg tcccggtgga
     3001 cgggcagggc aggatgcggc ccgaggcctt cccccgcatc gccggcccca ccatcgtctg
     3061 catccaggcg ggcaa
//
LOCUS       DBNGZC010000309         2875 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1100034, whole genome shotgun sequence.
ACCESSION   DBNGZC010000309 DBNGZC010000000
VERSION     DBNGZC010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1100034"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="AC16G0_05615"
     CDS             <1..932
                     /locus_tag="AC16G0_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5099135.1"
                     /translation="PAIYVEKYLRAPRHVEMQILADRHGHAIYLGERECSVQRRHQKV
                     IEEAPSPIVNAELRRRMGEAAVRLAVAAGYVNAGTVEFLVDPDRNFYFLEANTRIQVE
                     HPVTEMVTGLDLVKEQITIAAGEPLRLRQEDLQIRGAAIECRIYAEDPFRNFTPSPGR
                     ITSLRRPGGPGVRIDSGVDEGYDVPAHYDSLIAKLITWGQDRREAVQRMRRALTEYCI
                     QGIKTTIPFHRRVMADEDFLRGEIDTTFIDRRFPGSPHAWDESKREVALVAAAIHAFL
                     QDRSRPNPSLDAAARGISPWILAARQGGLRNFK"
     gene            1014..1517
                     /locus_tag="AC16G0_05620"
     CDS             1014..1517
                     /locus_tag="AC16G0_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR5099136.1"
                     /translation="MAYIASSRGQTHRLEVQQERDRLCVRLDGRDFPADVLQVAPHHY
                     SMLIQGRHYEIDVLEMEEASVVLVNGQPFRVDIQPEQMRYGRTQPRKTESQGGNKSVS
                     APMPGKVVKLLVKLGDVVREGDGIVVVEAMKMENELKAPVAGTVAEIRAGEGKAVNGG
                     DVLVVIE"
     gene            1618..>2875
                     /locus_tag="AC16G0_05625"
     CDS             1618..>2875
                     /locus_tag="AC16G0_05625"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HIR5099137.1"
                     /translation="MTKDEREKIEALRRKWEEGALKRTLERTPERQVEFRTTAGIRLE
                     RIYTPAEIADLDLERDLGFPGEYPFTRGVQPTAYRGRLWTMRQYAGFGTAEETNRRFR
                     YLLQQGQTGLSVAFDLPTQMGYDSDHPMVAGDVGKVGVAIDSLADMERLLEDLPLDQV
                     STSMTINATASILLCMYIAVAERQGIPCRQLAGTIQNDILKEYIARGTYIFPPAPSMR
                     LITDTFAFCAARLPRWNSISISGYHIREAGSTAVQEVAFTLADAIAYVEAAIRAGLDV
                     DGFAPQLAFFFNAHNNLLEEVAKFRAARRLWARIMRERFGAKDPRSWMLRFHAQTAGS
                     TLTAQQAENNVVRVAVQALAAVLGGAQSLHTNSRDEALGLPTEEAVRIALRTQQILAY
                     ESGVADVVDPLGGAYCVEALTDRIETE"
BASE COUNT          531 a          889 c         1016 g          439 t
ORIGIN      
        1 atccggccat ctatgtggag aaatacctgc gtgccccccg ccacgtggag atgcagatcc
       61 tggcggatcg gcacggccac gccatctact tgggggagcg cgagtgttcg gtccagcgca
      121 gacaccagaa ggtgatcgag gaggcccctt ctccgatcgt gaacgcagaa ctgcggcggc
      181 gtatgggcga ggcggcggtc cggctggcgg tggccgctgg ctacgtgaac gccggcaccg
      241 tggaattcct ggtggacccg gatcggaact tctacttctt ggaggcgaac acgcgcatcc
      301 aggtggagca tcccgtgacc gagatggtga cggggctgga tctggtgaaa gagcagatca
      361 ccatcgccgc gggagaaccc ctgcgcctcc gacaggagga cctgcaaatc cggggggcgg
      421 cgatcgagtg ccggatctac gccgaggatc ccttccggaa cttcaccccc tcgcccgggc
      481 ggatcaccag cctgcgcagg ccgggcgggc cgggggtgcg gatcgacagc ggcgtcgacg
      541 aggggtacga cgtcccggcg cattatgatt ccctcatcgc caagctcatc acctggggcc
      601 aggatcgtcg ggaggcggtg cagcgcatgc ggcgcgccct cacggagtac tgcattcagg
      661 ggatcaagac caccatcccc tttcatcgac gcgtcatggc agacgaggat ttcctccggg
      721 gcgagatcga cacgaccttc atcgatcgcc gcttccccgg gagtccccac gcctgggacg
      781 agtcgaagcg agaggtcgcc ctggtggccg ccgcgatcca cgcttttctc caagaccggt
      841 cacgccccaa cccgtcgctg gatgcagcgg cacggggaat cagcccctgg atcctggctg
      901 cgcgccaagg cgggcttcgc aatttcaaat gagaaactga caactgacaa ccggccaatg
      961 tccggaaatg gtcggttatc agttgtcggt tttcaattgc agccggaggc ttgatggcct
     1021 acatcgcgag ttcacggggg cagacacacc ggctggaggt ccagcaggag cgtgacaggc
     1081 tctgcgtgcg cctggatggc cgggacttcc cggcggatgt cctgcaggtg gccccgcacc
     1141 actattccat gctcatccag ggacgccact acgagatcga cgtcctggag atggaagagg
     1201 cctcggtggt gctggtcaac gggcagccct tccgtgtgga tatccagcca gagcaaatgc
     1261 gttacggccg aacccagccc aggaagacgg aaagccaggg ggggaataag agcgtgagcg
     1321 cccccatgcc gggcaaggtt gtcaagctcc tggtcaagct tggagacgtg gtgcgggagg
     1381 gagacgggat cgtcgtggtg gaagccatga agatggagaa cgagctgaag gcccccgtgg
     1441 caggaacggt cgcggagatc cgggcggggg aaggcaaagc ggtgaatggg ggtgatgtgc
     1501 tggtcgtgat cgagtaggaa ccggaggcgc gaggagagac cggcaacagg ggagcgggcc
     1561 aggttccgcc aggcggtccg gggcggaggg ggcagcggtg caggaagggc aggccaggtg
     1621 acgaaggacg agcgcgagaa gatcgaagcg ctgcggcgga agtgggagga gggggcgctc
     1681 aagcggacgc tggagcggac gccggaacgg caggtggagt tcaggacgac ggcggggatc
     1741 cgcctggagc ggatctacac gccggcggag atcgccgacc tggacctgga acgagacctg
     1801 ggcttccccg gcgagtatcc cttcacccgg ggcgtccagc ccaccgcgta ccgcggacgc
     1861 ctgtggacca tgcggcagta cgccggcttc ggcactgccg aggagaccaa ccgccgcttc
     1921 cggtatctgc tccagcaggg acagaccggc ctgtcggtgg ccttcgacct gccgacgcag
     1981 atgggctacg actccgacca ccccatggtg gccggcgatg tgggcaaggt cggcgtggcc
     2041 atcgattcgc tggcggacat ggagcgactg cttgaggatc tgcccctgga ccaggtcagc
     2101 acctccatga cgatcaacgc caccgcgtcg atcctgctct gcatgtacat cgccgtggcc
     2161 gagcggcagg ggattccttg cagacaactg gccggcacga tccagaatga tatcctgaag
     2221 gagtacatcg cccgcggaac ctacatcttc ccgccggccc catccatgcg gctcatcacc
     2281 gacaccttcg ccttctgtgc ggccaggctc ccccgttgga acagcatcag catcagcggc
     2341 taccacatcc gcgaggcggg ctccacggcg gtgcaggagg tggccttcac gctcgcggat
     2401 gccatcgcct acgtcgaggc ggcgatccgg gcgggactgg atgtggacgg cttcgccccg
     2461 cagctcgcct tcttcttcaa cgcgcacaac aacctgctgg aggaggtggc gaagtttcgg
     2521 gccgcccggc gactctgggc ccgcatcatg cgtgagcggt tcggggccaa agacccccgc
     2581 tcctggatgc tccgctttca cgcccagacc gcgggatcga cgctcacggc gcagcaggcg
     2641 gagaacaatg tggtgcgtgt ggcggtccag gctctggcgg cggtcctggg cggggcgcag
     2701 tcgctgcaca cgaactcccg cgacgaggcc ctgggcctcc ccaccgagga ggcagtgcgg
     2761 atcgcgctcc ggacccagca gatcctggcc tacgagagtg gtgtggcgga cgtggtggac
     2821 cccctgggtg gcgcgtattg cgtggaagcg ctgacggatc ggatcgagac cgaag
//
LOCUS       DBNGZC010000310         2302 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2079843, whole genome shotgun sequence.
ACCESSION   DBNGZC010000310 DBNGZC010000000
VERSION     DBNGZC010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2079843"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="AC16G0_05630"
     CDS             complement(<1..362)
                     /locus_tag="AC16G0_05630"
                     /inference="COORDINATES: protein motif:HMM:NF024751.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR5099138.1"
                     /translation="MLDETIITETPPLYSCYGHLGAQVRVPITGNRVKRILHGASNVR
                     TGDVSLLITKEWTKEMHQGFLSMIRSHWRGWNIVLFEDRASQHTSPDSLGWAEELGIE
                     VRLLPKATPELNAMDHLW"
     gene            complement(389..901)
                     /locus_tag="AC16G0_05635"
     CDS             complement(389..901)
                     /locus_tag="AC16G0_05635"
                     /inference="COORDINATES: protein motif:HMM:NF024954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5099139.1"
                     /translation="MTSIILSDRLRGELEDLVIHTSLAKERSRAQALLWLADGESVEQ
                     AAELLRVSRQTVYNWVERFRQREGLDLRARLLDAPRPGRPSTALGIIDPLIEAVIDQD
                     PRELGYHSTVWTTALLQQHLKRAHGIAVSRKSISLAIVRLGIRWQRPRHQLALRPKTW
                     RQSKGGGNAA"
     gene            complement(966..1202)
                     /locus_tag="AC16G0_05640"
     CDS             complement(966..1202)
                     /locus_tag="AC16G0_05640"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5099140.1"
                     /translation="MARDPMDASVGLLRALADPVRLRLLNLLGAREEVCVHHLHEALE
                     LPQPTVSRHLAYLRKHGLVIGRKEGLWVHYRLAK"
     gene            complement(1249..1974)
                     /locus_tag="AC16G0_05645"
     CDS             complement(1249..1974)
                     /locus_tag="AC16G0_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099141.1"
                     /translation="MASAALLLAISDLSTRSSTLAVFTVLGLIGVAVLGVLYYRRSQL
                     RGAVDAQFRGFREQAVALMDQIDSLRQRHNTLPSTDPDFTAPMTGATLALYNQVEHDL
                     DDLWSRWLGVMETWDQVERQIRAGSGLTLRPTEEARKLIEQGGIDMLLRQSSACRQKL
                     DRLNQGHEQAREALAAGRAELKALHDAPELLDRLDRAGEIARAVASSERLFAEAERLI
                     TADPIGTEGRTAMFSTVALVRKA"
BASE COUNT          394 a          764 c          741 g          403 t
ORIGIN      
        1 ctccacaggt ggtccatcgc gttcaactcc ggcgtcgcct tgggcagcaa ccggacctcg
       61 atgcccaact cctcggccca tcccaggctg tccggcgacg tgtgctggct ggctcgatcc
      121 tcgaagagca cgatgttcca accccgccag tgggagcgga tcatcgacag gaagccttgg
      181 tgcatctcct tggtccactc cttggtgatc aacagcgaca catctccggt ccggacgttg
      241 ctcgccccgt ggaggatgcg cttgacgcgg ttccccgtga tcggcactcg gacctgcgcg
      301 ccgagatggc cgtagcagga gtagagcggc ggggtctcgg tgatgatcgt ctcgtcgagc
      361 atcaacacca cggtgcgggc acggtccttc aggccgcgtt tccgccccct tttgactgcc
      421 gccacgtctt gggacggagt gccaactggt gccgaggccg ctgccaccgg atacccaggc
      481 gtacgatcgc caagctgata ctcttgcgag agaccgcgat cccatgagcc cgcttcagat
      541 gctgctggag cagcgccgtg gtccagaccg tggaatgata acccagttcg cgtgggtcct
      601 gatcgatgac ggcctcgatc aacggatcga tgatccccag ggccgtggaa gggcgaccgg
      661 ggcgaggggc atcgaggagt cgtgccctga ggtcaagtcc ctcacgctgc cggaatcgct
      721 cgacccagtt gtaaaccgtc tgacggctga cgcggagcag ttcggcagcc tgttcgacgg
      781 actccccgtc ggcgagccag agcagggctt gagcccggct tcgctccttg gccaaagagg
      841 tatggatcac gaggtcttcc aactctccac ggagtcgatc gctcaggatg atcgaggtca
      901 tcgggccacc cccgaattct ggtgactcaa tggccgattc taattgtcaa agaacttttt
      961 gccacctact tagcaagccg gtagtggacc cacaggccct ccttgcggcc gatgaccagg
     1021 ccgtgtttcc tgagataggc caggtgccgc gacacggtcg gctggggcaa ttccagggcc
     1081 tcatgcaggt ggtggacgca gacctcttcg cgagcgccga gcaggttcaa gagccgtagc
     1141 cgaaccgggt cggccagcgc ccggagcagc ccaaccgaag catccatcgg gtcccgcgcc
     1201 atgatccatg ccgtcccgct tcaggccgcc gcggttgctt gggaggagtc acgcctttcg
     1261 cacgagggct acggtactga acatggccgt cctcccctca gtgccgatcg ggtcggcggt
     1321 gatcaaccgt tcggcctccg cgaagaggcg ctcggacgac gcgaccgccc gggcgatttc
     1381 tcccgcccga tcgagcctgt cgagcaactc gggcgcgtcg tgcagggcct tcagctcggc
     1441 ccggcccgcg gccagggcct cgcgcgcctg ctcatgcccc tggttcaggc ggtcgagctt
     1501 ctggcggcac gcactcgact gccgcaggag catgtcgatc cctccttgct cgatcagctt
     1561 ccgggcctcc tcggtcggcc tcagggtcag gcccgagccg gcgcggatct gccgctcgac
     1621 ctggtcccag gtctccatca cccccaacca gcggctccag aggtcgtcga ggtcgtgctc
     1681 gacctggtta tagagcgcca gcgtggcgcc ggtcatcggc gccgtgaagt cggggtcggt
     1741 gctgggcagg gtgttgtgcc gctgcctcag gctgtcgatc tgatccatga gcgccacggc
     1801 ctgctcgcgg aatccccgaa actgcgcgtc cacggcgccc cggagctggc tcctgcgata
     1861 gtagagcacg cccagcacgg ccacgccgat caggcccagg accgtgaaaa ccgccagggt
     1921 ggagctgcgc gtggagagat cgctgatcgc aagcaacaac gccgcggatg ccattcggtt
     1981 ccctgactga gcaatcaagg agtgcatcag aaaagactcc agatcgtgcc cggcggacca
     2041 agcggccacg cggaacgagg actgagtaaa gagcggcggt ctcctccgcg cagatcgacc
     2101 atggccacgg tatataggag cgcctcacag cgacccaccc ccgccgcggc tggcgctgac
     2161 gatgaccacg ggccggtcac gattgtattc ggtttgggcg gtgcggacag ccttgtcccg
     2221 gtcgtgatcg gagagggcac aatcacgctt cctctcggtt acactgtggg cgtggcgatg
     2281 agacttccgt aggggagcga ca
//
LOCUS       DBNGZC010000311         1844 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2079871, whole genome shotgun sequence.
ACCESSION   DBNGZC010000311 DBNGZC010000000
VERSION     DBNGZC010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2079871"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(404..1726)
                     /locus_tag="AC16G0_05650"
     CDS             complement(404..1726)
                     /locus_tag="AC16G0_05650"
                     /EC_number="5.4.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR5099142.1"
                     /translation="MRSQELYAAPQRHLIGGAGAGGRYHPLLRRPLYLARGKGSRFWD
                     VDGQEYIDFSTGSGANFLGHDHPAITAAIRQALEVGVICNGETEHHSRLAELVSDAVP
                     CAQKVRFANSGTEATMGAIRIARGYTGKPKILKFEGHFHGMHDYLWYNCSTALGDLRE
                     DGTIAPVPDTDGMPEALSDLILVVPFNDPAAFDRAVTAHKDELAAVIMEPISYNQGCI
                     PAKPEFLRQVREICTRAGIVLIFDEVLSGFRMGRGGAQEYYGVTPDLCTLAKALGGGV
                     PIAAVCGTTEVMSVLNPIGHTIMSGTYTGHLTAVMGAIACQNEIAKPGFYAHINTLAD
                     RLYRGMTEALRATGVPGIVQGIGARFGLYLGVTEPVTNYREAARCSRDMEIRFLLGCV
                     KRGLYLHDYAHKTPMHHGFSSQHSIQDIDEALNIMQDSLKDLTRVRGE"
BASE COUNT          317 a          571 c          602 g          354 t
ORIGIN      
        1 cctggcgggc gaagcgcagc gtatcgggat tcagaatcgt tcggtacagt cccaggccga
       61 gtttcatggc attttctcgc tccagcgatg caagggccac ggcgccggaa gcgccaagac
      121 aagagcatgg ccctgaccag agcggacgat acggtactgg cggaggaagg tcaagccagt
      181 cgcggggagc gtgaccgagc cggggggttg cggacagtct agggtcggcg tcatcgggca
      241 gggtcttgtc gcgtgtcctc cttttgacgg gcctcttcct gcgccgcgtt gaagcaggcg
      301 tagcagatgg gacctttctc agtcatcatc ttctggtgct cgcgtctcgg tccgagatcg
      361 cgaccgcact tccagcacgt ggccatcacg acccttccct ccttcattcg cctcggacgc
      421 gcgtgaggtc cttgagggaa tcctgcatga tgttcagcgc ttcgtcgatg tcctggatgc
      481 tgtgctggga tgaaaatccg tggtgcatcg gcgtcttgtg ggcgtagtcg tgcaaataca
      541 ggccgcgctt cacgcagccc aggagaaacc gaatttccat gtcgcggcta catctggcgg
      601 cttcccggta gttggtcacc ggctccgtta ctccgaggta gagaccaaat cgggcgccga
      661 tgccctgcac gatgccggga acgcctgtgg ctcgcagggc ttcggtcatc ccccggtaga
      721 gcctgtcggc cagtgtattg atatgcgcat agaatcccgg cttggcgatt tcgttttggc
      781 aggcgatcgc tcccatcacg gcggtgagat gccccgtgta cgtaccgctc atgatagtgt
      841 gcccgatggg attcagcacc gacatgacct cggtcgtgcc gcagacggct gcaatcggga
      901 cgccgccacc gagcgccttg gccagcgtgc agaggtccgg ggtcacgccg taatactcct
      961 gcgccccgcc gcgacccatg cgaaagcccg aaagcacctc gtcaaagatc aggacgatgc
     1021 ctgcgcgggt gcagatctcc cggacctgcc gcaggaattc cggcttggcc gggatgcagc
     1081 cctgattata ggaaatcggc tccatgatca ccgcagccag ttcatccttg tgggctgtga
     1141 ccgctcggtc aaaagctgct gggtcgttga aagggaccac caggatcagg tccgacaggg
     1201 cctcaggcat cccgtcggta tcgggaacgg gcgcgatggt cccatcctcg cgaaggtcgc
     1261 cgagggcggt cgagcagttg taccacaggt aatcatgcat tccatgaaag tggccctcga
     1321 acttcaggat cttgggcttc ccggtgtagc cgcgggcgat gcggatggcc cccatggtcg
     1381 cctcggtgcc gctgttggcg aagcgcacct tttgggcgca cgggaccgcg tcggagacta
     1441 gctcggccag gcgggagtgg tgctccgtct cgccgttgca gatcaccccg acctccaggg
     1501 cctggcggat ggcggcagtg atcgcggggt gatcgtggcc gaggaaattg gcgccgctgc
     1561 cggtggagaa atcgatgtac tcctggccgt ccacatccca gaagcgggag cctttcccgc
     1621 gggccaggta cagaggacgt ctcagcagcg ggtgataccg tccgcccgcc cccgccccgc
     1681 cgatcaggtg tctttggggc gccgcgtaca gttcctgcga tctcatggcc tcctcctcgc
     1741 ggtctgacga atgggaatca gtgagtcaga tcctcccgcg cagcgcgcgc tcctgggagc
     1801 acgatctggc catggcggga aacgatctgc ccgtcccgga gaac
//
LOCUS       DBNGZC010000312         3259 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2275704, whole genome shotgun sequence.
ACCESSION   DBNGZC010000312 DBNGZC010000000
VERSION     DBNGZC010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2275704"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..628
                     /locus_tag="AC16G0_05655"
     CDS             146..628
                     /locus_tag="AC16G0_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010844624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HIR5099143.1"
                     /translation="MTVRYVTRKRPQVDRCASAWLLTRFVDPQAEFAFVEEGDTLPGA
                     IPFDMSGVELGHHRGRCTFEAILRKHKLRDPALVELGRIIRSADLLDAEETLEGPGID
                     LLFRGLLHLTGDDQEILRLAIPILEALYAALQQRGRSRPQRPSEPRGQTHGRGSAGGR
                     "
     gene            739..1179
                     /locus_tag="AC16G0_05660"
     CDS             739..1179
                     /locus_tag="AC16G0_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HIR5099144.1"
                     /translation="MKWVTREGAKTDRVACPWLIRKFIDPTAAFLFVPKEQVLAIAAR
                     EGGKSFDCPGADYTHKDGKCSFEALVDAHRLMDPALRLLASIVHGADVAADVGIVPEA
                     AGLQAIAHGFAAICTDDHRKLELEFPVYDALYAWCQAKAAGKSI"
     gene            1267..2439
                     /gene="chrA"
                     /locus_tag="AC16G0_05665"
     CDS             1267..2439
                     /gene="chrA"
                     /locus_tag="AC16G0_05665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HIR5099145.1"
                     /translation="MSEAASRPPVRIRDLVRYYLRLGTIGFGGPVALCGQMEQELVQD
                     KQWLTKAELREGIAVCQSLPGPLAIQVGIYVSYLRGGFWGAWAGGWAFILPNFLIVAT
                     LGALYVHFGDLPPVRAIFYGVSPAVIALILHSCYRLAKLGMEDAFQWVIATICLVVTV
                     VVQAEVALLFISAGALGVLYYGTSWRSRVPSFPFFAAAPVALGAAHSGTLLAKLLAFF
                     VKAGSLTFGSGLVIVPFLENGLVRQTGWLDERQFLVAVAMGMLSPGPVVITATFVGYL
                     VAGFWGSVVSTVGIFLPSFLLVLLVAPVLIRHRGNPQVQGFVKGAYAAAIGTILGASI
                     LLGRIAIGDWLTALVGIVSLGVLFKWKVSNPLLVAATAAVGLVAFPLIHPSWVFVK"
     gene            2584..>3259
                     /locus_tag="AC16G0_05670"
     CDS             2584..>3259
                     /locus_tag="AC16G0_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR5099146.1"
                     /translation="MTLEQRGYVPLPAHTGRGGFDHAAVHHAGGRLYVAHTANDALDV
                     IDCCTGRYVYSVPDLPGVAGALVCEEQDLLFTSNRREHTVGIFSAPEVRLLGKVRVGL
                     GPNGLAYDPGRGRLLAANVGDDAIPGSHTVSIVDVKTRTLIGSVPVAGRTRWALFDAA
                     SDAFYVNIADPPEIAVIGADRPDHVHRTIPIPAAGPHGLDLDPGRRRLFCACDAKLVV
                     ALEPISG"
BASE COUNT          497 a         1081 c         1098 g          583 t
ORIGIN      
        1 agctccgccg gatccgccag gctgccctca agctgcgccg gcgagcccat gttccggtcc
       61 gagaggaaga aacactccag cggcgggtgc gtgcggtacg ggaccggctg gaccggctcg
      121 cactccgggc cacccggcaa agggaatgac cgtgcgctac gtgacccgga aacgtcccca
      181 ggtggaccgg tgtgcgtcgg cctggctcct cacccgcttc gtcgatcccc aggcggagtt
      241 tgccttcgtc gaggaggggg acacgctccc cggagcgatc ccctttgaca tgtcgggtgt
      301 ggagctcggc caccaccgcg ggcgctgcac cttcgaagcg atcctgcgca agcataaact
      361 cagggatccc gcactggtcg agctgggccg gatcattcgg agcgcggatc tcctcgacgc
      421 cgaggagacc ctggaggggc ccgggatcga cctcctcttc cgggggctgc tgcatctgac
      481 gggagacgat caggagatcc tgcgcctcgc gataccgatt ctcgaggcgc tctacgccgc
      541 tctccagcag cgcggcagga gccgtccaca gcgtccatcg gagccacggg gccagaccca
      601 cggtcgaggg tccgcgggag gcaggtaatc cagcggggat ccggtgtcac gcccgctgcg
      661 agggtgggac tagggggaaa cgacacacgt gtcttgctgc cggcggggca gagagtccgg
      721 cagattgcga gggcgatcat gaaatgggtg acgcgagaag gggccaagac ggaccgcgtg
      781 gcgtgtccgt ggttgatcag gaagttcatc gatccgaccg cagcctttct gttcgtgcca
      841 aaagagcagg tcctggcgat cgcggctcgg gagggcggga aatcctttga ctgcccgggg
      901 gcggactaca cgcacaagga cgggaagtgc agcttcgagg cgctcgtgga cgcgcaccgc
      961 ttgatggacc ctgcgttgcg cctcctcgcc agcatcgtcc acggcgccga tgtggcggcg
     1021 gatgtcggga tcgtgccgga agcggcgggc ctccaggcga tcgcgcacgg ctttgcggcc
     1081 atctgcaccg acgaccacag gaagctcgag ctggagttcc ccgtgtacga cgcgctctac
     1141 gcctggtgcc aggccaaagc cgcggggaag tccatctgag cttgatgtcc ccagggtaga
     1201 cctgaacacg tcacaccacg ggccacccaa cgcctggtgg attcccgaaa ggggaagggg
     1261 gtacccatga gcgaggcagc gtctcggccg cccgttcgca ttcgtgacct cgtccgctac
     1321 tacctgcgcc tcggcaccat cgggttcgga ggacccgtcg cgctctgtgg ccagatggag
     1381 caagaactgg tccaggacaa gcagtggctc acgaaggccg agctacggga gggaatagcc
     1441 gtttgccagt ccctgccagg gccgctagcc atccaggtgg ggatctatgt gtcctacctc
     1501 cgcggcggct tctggggtgc gtgggccggc ggctgggcct tcatcctccc gaacttcctg
     1561 atcgtggcca cgctgggtgc cctctacgtc cacttcggag acctgccgcc ggtccgcgcc
     1621 atcttctacg gcgtaagccc ggcggtcatc gccctgatcc tccactcctg ctaccggctg
     1681 gcgaagctcg ggatggagga tgcgttccag tgggtcattg ccacaatctg cctcgtggtc
     1741 acggttgtcg tgcaggccga ggtggcactc ctcttcatct cggcgggcgc gctcggggtc
     1801 ctctactacg gaacctcatg gcggtcccgg gtcccgtcct tccccttctt cgccgcggcc
     1861 ccggttgcct tgggcgccgc ccactcgggc acgctcctcg caaagctcct ggccttcttc
     1921 gtgaaggccg gctccctcac cttcgggagc ggcctcgtca tcgtgccctt cttggagaac
     1981 ggcctggtgc ggcagacggg ctggctggac gaacggcagt ttctcgtggc ggtggctatg
     2041 ggtatgctga gcccgggccc agtcgtcatc accgcaacgt tcgtgggcta cctcgtggcc
     2101 ggcttctggg gctcggtcgt ctccacggtg ggaatcttcc tgccctcgtt cctgctggtc
     2161 ctgctcgtcg ctcccgtcct gatccggcac cggggcaacc cgcaggtcca gggattcgtg
     2221 aagggagcct atgccgccgc catcggcacg atcctgggag cctccatcct cctgggcagg
     2281 atcgccatcg gcgactggct caccgccctg gtcgggattg tgagcctcgg cgtgctgttc
     2341 aagtggaagg tgagtaaccc gctcctggtc gcagcgacag cggccgtggg gctggtcgcc
     2401 tttccactca tccacccgtc ctgggtgttc gtgaagtagg ccggggcggc atggaagata
     2461 ggagaagggc atgaacatgc atcactgggt cttcgtctgc cttcatggcg ccgccaagag
     2521 cgtcatggcg gcggaagtca cctcgagcgg gatcatcgca cgcggatagg aaggggcata
     2581 gccatgactc tcgaacagcg cgggtacgtt cctcttccgg cccacactgg gcggggcggc
     2641 ttcgaccacg cggccgtgca ccatgccggc ggccgcctgt acgtggccca cacggcgaac
     2701 gatgcgctcg acgtgatcga ctgttgcacc ggtcggtatg tgtattcggt gccggacttg
     2761 cctggggtgg cgggcgcgct ggtgtgcgag gagcaggatc tgctcttcac gtcgaaccgg
     2821 cgcgagcaca ctgtcggcat attctcggca cccgaggtgc gccttcttgg caaggtgcgt
     2881 gtcggcctcg gacccaacgg cctggcgtat gatccggggc gggggcggct cctggccgcc
     2941 aacgttggag acgacgccat tccagggtcg cacacggtct ctatcgtgga tgtcaagacc
     3001 cgcaccctga tcggctccgt tcctgtggcg ggacgaaccc gttgggcgct ctttgatgcc
     3061 gcctcggacg ccttctacgt gaacatcgcc gacccgcccg agatcgcagt catcggcgcc
     3121 gatcgccccg atcacgtgca ccggactatt cccatcccgg cggccgggcc gcacggcctg
     3181 gatctcgacc cagggcgccg ccggctcttc tgcgcctgtg acgcaaagct cgtcgttgcc
     3241 ctcgaaccca tcagcgggc
//
LOCUS       DBNGZC010000313         3114 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1786659, whole genome shotgun sequence.
ACCESSION   DBNGZC010000313 DBNGZC010000000
VERSION     DBNGZC010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1786659"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..768)
                     /locus_tag="AC16G0_05675"
     CDS             complement(58..768)
                     /locus_tag="AC16G0_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099147.1"
                     /translation="MRPVLEIRQLETGYDLSQALFGIDLAIGEGEVAALLGRNGTGKT
                     TTVRAVLGLCRTWRGTIAFRGERIDAIETDAIARRGIALVPEGRQIFPNLTVREHLVA
                     FGDNRSGTGDPWTPERVFALFPQLAVRADAPGVQLSGGEQQMLAIGRALVTNPHLLIL
                     DEATEGLAPLLRKEVWRCLTSLKASGQTILVIDRSVEALVEIADHHTIIERGRVVWRG
                     TSAALAAEPAVWQRYLGV"
     gene            complement(765..1523)
                     /locus_tag="AC16G0_05680"
     CDS             complement(765..1523)
                     /locus_tag="AC16G0_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099148.1"
                     /translation="MTTILETRELCKRFGRLVVTDHCSLSVADGETHAVIGPNGAGKT
                     TLIAQLSGALSCDEGEVWFAGRNVTGLRMHARVAMGLVRSYQITSLFPGFTVRENLAL
                     AVQARSGSSMRFWRPVREEIAQSREAERIGVELGLGDRLDTPASRLAHGEQRQLEVGL
                     ALATQPRLMLLDEPMAGLDPEESLRMINLLGTLRGRRALLVVEHDMDAVFRLADRVSV
                     LVAGRVIATGTPADVRDNPDVRQAYLGDEWALIA"
     gene            complement(1520..2461)
                     /locus_tag="AC16G0_05685"
     CDS             complement(1520..2461)
                     /locus_tag="AC16G0_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5099149.1"
                     /translation="MIHETRTGFALLSLGVLLALPLAARTLGDTFYISVASRVLIYGL
                     AAASLNLILGFGGMVSFGHAAFVGAGAYTVGILAQEAIRSAWIAWPAAVLVSGLLALL
                     IGAISLRTRGLYFIMITLAFAQMLYYFFVSLKRYGGDDGLALPARSGLGLGLELRDDL
                     TFYYATLSILVLALWLCHRLLQARFGQAIRGIRENEARMEAIGFPVYRYKLAAFVIAG
                     STAGLAGALLANQGRFVSPTLLSWQLSGILIVMVILGGAGRLTGPPVGAGVVLGLEEI
                     LTASTQHWQLGLGLLLLLVVLCAPGGITGLAAGRAPR"
     gene            complement(2770..>3114)
                     /locus_tag="AC16G0_05690"
     CDS             complement(2770..>3114)
                     /locus_tag="AC16G0_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5099150.1"
                     /translation="TAVFGLGAALCAAAGALLGPLLAVQVGMGESILILCFVVVIIGG
                     VGSIRGALVGAILVGMVDTFGRVALGMLLRQLLPHSVAVNVGPPLASMSIYLVMAGVL
                     SWRPQGLFPARG"
BASE COUNT          564 a         1068 c         1051 g          431 t
ORIGIN      
        1 ggatcgaccg ggataccgcc agagaccgga gccggaggct ccgggggccg gaggcgctca
       61 gacgcccaga tagcgttgcc agaccgcggg ctccgcggcg agcgccgcgg acgttccgcg
      121 ccacaccacc cggccccgct cgatgatggt gtggtggtcg gcgatctcga ccagggcttc
      181 gacggatctg tcgatgacca ggatcgtctg gcccgaggct ttgagcgagg tcaggcagcg
      241 ccagacctcc ttgcggagca ggggggccag gccctcggtg gcctcgtcca ggatcagcag
      301 gtgtgggttg gtcaccagcg cgcgaccaat ggccagcatc tgctgttcgc cccccgaaag
      361 ctggactccc ggagcgtccg cccggaccgc cagctgaggg aacagcgcga agaccctctc
      421 cggggtccag gggtcaccgg tcccgctccg gttgtcacca aacgccacga gatgctcccg
      481 caccgtcagg ttcggaaaga tctgacggcc ctcggggacg agggcaatcc cacggcgcgc
      541 gatggcatcg gtctcaatgg catcgatccg ctcgccccgg aacgcgatgg tcccgcgcca
      601 ggtgcggcag agccccagca cggcgcggac ggtcgtggtc ttccccgtgc cgttgcgtcc
      661 caggagcgcg gcgacctctc cctctccgat ggcaagatcg attccgaaga gcgcctggct
      721 caggtcgtag ccggtctcca actgccgaat ctccaacacc ggcctcatgc gatcagtgcc
      781 cactcgtccc ccagataggc ttgccggaca tccgggttgt cgcgaacgtc ggccggtgtg
      841 ccggtggcga tcacccttcc cgccacgagc acggacacgc ggtccgcgag ccggaacacc
      901 gcatccatgt catgctcgac gacgaggagc gccctccgtc ccctcagcgt gccgagcaga
      961 ttgatcatgc gcagcgactc ctcgggatca aggcctgcca tgggttcatc caggagcatg
     1021 agcctgggtt gggtcgccag cgcgagaccc acctcgagtt gccgttgctc gccatgggcg
     1081 agccgggaag ctggggtgtc caggcggtcg cccagcccaa gctcgacgcc aatccgctcg
     1141 gcttcccgac tctgcgcgat ctcctcccgg accggtcgcc agaaccgcat gctcgatccg
     1201 cttcgggcct ggacggcgag ggcgagattc tcgcggaccg tgaaccccgg aaacaggctg
     1261 gtgatttgat aagagcggac caggcccatc gccactcggg cgtgcatgcg gagcccggtg
     1321 acgttgcgtc ctgcgaacca gacttccccc tcgtcgcagg acagtgcacc cgagagctgg
     1381 gcgatcaggg tggtcttgcc ggcgccgttc ggcccgatga cggcatgggt ttccccgtcg
     1441 gcgaccgaca ggctgcagtg gtcggtcacg accagtctgc cgaagcgctt gcagagctcc
     1501 cgggtctcga ggatcgtcgt catcgcgggg cccgtccggc cgccagcccg gtgatgcccc
     1561 ccggggcgca gaggaccacc aggagcagga gcaggcccag tccaagctgc cagtgctggg
     1621 tggaggccgt gaggatctcc tccagaccca acaccacccc ggcgccgacc ggcggtccgg
     1681 tgagacggcc ggcgccgccc aggatcacca tcacgatcag aatccccgat agttgccagc
     1741 tgagcagggt cgggctgacg aagcggccct ggttggcgag cagggcgccg gccaggccgg
     1801 cggtcgaccc ggcgatgacg aaggccgcca gcttgtaccg gtagacggga aagccgatgg
     1861 cctccatccg cgcctcattt tcccgaatcc cgcggatcgc ctgaccgaag cgggcctgca
     1921 gcagccgatg gcagagccac agggcgagca ccaggatgga cagcgtcgcg tagtagaagg
     1981 tgaggtcgtc gcgaagctcc aggccgagtc ccaggccgga gcgggcaggg agggcgaggc
     2041 cgtcgtcgcc gccgtagcgc ttcagggaca cgaagaagta gtacagcatc tgggcgaagg
     2101 ccagggtgat catgatgaag tagagacccc gcgtgcgaag cgagatcgcc ccgatcagca
     2161 aggccagcag gccgctcacc aaaacggccg cgggccaggc gatccacgcg ctgcggatgg
     2221 cctcctgcgc caggatgccg acggtgtagg cgccggcacc cacgaaggcg gcatggccga
     2281 agctcaccat gcccccgaac ccgaggatca ggttcaggct tgccgccgcc aggccgtaga
     2341 tgagcacccg gctggccacg ctgatgtaga acgtatctcc caacgtgcgg gccgcgagcg
     2401 gcagggcgag cagcacgccg agggagagga gtgcaaagcc ggtgcgagtc tcgtggatca
     2461 ttcctctcac ctgggtccca aaatggacct agatccgaga ttcggcatct cgccaagcac
     2521 gcaaagctcg ccaagaccga cgctcataat atgggtacag aaggcccaga aatttccccc
     2581 cctggaagcg gtcatccggg atgggcgatg ccgtgattga atcttgtcct tctttgcgcc
     2641 cttggcgtct ttgcgagaga cgggtttctt cggattgagg gggaatcctc cgtcccgcgg
     2701 aatgaaaggc ccgggtcagc gcccggcacc ggccgcgacg cccagcccta gccatggcgt
     2761 tccggtggat cagccccgcg ccgggaagag cccctggggc cgccaggaca gcacccccgc
     2821 catgacgagg tagatcgaca tggacgcgag cgggggaccg acgttgacgg cgacagagtg
     2881 cggcaggagc tgccggagca gcatgccgag cgcaacgcga ccgaaggtgt ccaccatgcc
     2941 gaccaggatc gcacccacca acgccccgcg gatagagccg acgccgccga tgatcaccac
     3001 gacgaagcac aggatcagga tgctttcgcc catcccgacc tgcaccgcga ggagcggccc
     3061 aagcagggcc ccagccgcgg cgcacagcgc cgcccccagg ccgaagaccg cggt
//
LOCUS       DBNGZC010000314         7018 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1100057, whole genome shotgun sequence.
ACCESSION   DBNGZC010000314 DBNGZC010000000
VERSION     DBNGZC010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7018)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7018)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7018
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1100057"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..2213)
                     /locus_tag="AC16G0_05695"
     CDS             complement(294..2213)
                     /locus_tag="AC16G0_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR5099151.1"
                     /translation="MCRRSLWWALMILLGTGGWASTAGGQQQPPEAEQTLEEVVVTAA
                     RIPEALSKTTADVTVITQQELRRRQITTVLEALRTVPALNVVQSGTPGSVTSVFSRGG
                     NSSHTLVMIDGVRVNSPTNGIFDFANLTTEGVERIEVVRGPASALYGSDGLTAVINIV
                     TRKGDGAPRGTVRVDAGSYQTFREQGQSSGKVGPVGYAVDVARTDSSGKFPHDEYNNT
                     SSAARLDYQALSNLSFDLSARYTHAFKNLPAVPGRTDFNTGLPDGTQSSREDLTSVTL
                     GGTHTPTSWWDHRLQVSLVQDLVDFRDPLAAFPSPPNLAFSTPFDTETTRLAADWQHN
                     IHPVSWNTLTLGFQVDHAKANQMDQFATRVVHMAISDYAFYGQNQATLWDRLILTAGV
                     RVDENSSFGTHVSPKFSAAFLVPETGSKLRGSYGEGFRAPSINDLAFPGFANPNLKPE
                     KNRSLEFGGDQSLWHDRIQAGVTWFQSRFRDLIQPSSGPLFIPVNVAHARTQGVEVTL
                     GFRPGYGLELAGSYTYLQTLDQNNQHLLRRPANTWNVAVNYAPWERINLNSTLLYVGK
                     RNDIDPTTFATTTNFKYVLWNAGAAVKVLDQVGLLRRLTAFVKLENILGDRYEEALGF
                     PALGRRYLIGMSGDF"
     regulatory      complement(2238..2450)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(2426..3247)
                     /locus_tag="AC16G0_05700"
     CDS             complement(2426..3247)
                     /locus_tag="AC16G0_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099152.1"
                     /translation="MKALEVEGVGFWYRTGQGVEDVTFQAAPGDLLGVVGPNSAGKST
                     LLRLLSKVVVPEHGRILIGGRDVARLSRLELARSLAVVPQEFSVAFPFSVAEVVLMGR
                     YPHAAGGSWATSKDRAVAEAAMEATGILALAGRRVDELSGGERQLVSIARALAQEPSI
                     LLLDEPTTHLDLGHQRTVLEILLAHHREGRQTAVLVSHDLNLAAEYCDRILLLVEGRV
                     RAFGTPEEVITEEHLTSAYGCPVEVERHPASGRPRVHGSLPREHTNGPQLTTPRM"
     gene            complement(3321..4370)
                     /locus_tag="AC16G0_05705"
     CDS             complement(3321..4370)
                     /locus_tag="AC16G0_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013219.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR5099153.1"
                     /translation="MSLRLSASIGKGMGWQPAAFALLLAILLLGLLLSVMVGSLPVAP
                     RDVGRALLGSGVRADDPVVTVIRQIRLPRAVLAGIVGACLSLAGLGFQAISRNPLADP
                     TVLGVSSGASFGVTLAVLLGLAGPFANPAVVTLFAFGGALLAAVAVYAIARVEGRLPI
                     TTLLLSGVVVGLFFNACVMLATALLASAELQGVVFWLMGNLGPARVRTLAILALALLI
                     GAAVLTRQAARLNLLALGEEQALQLGVEAERVKRLVFVAASLVTGAAVSAAGSIGFVG
                     LIVPHAARLLLGPDNRRLVPATALLGASFLMLADLVARTVAAPSELPVGVITAFCGAP
                     LFVYLLRTRRGGDPL"
     gene            complement(4367..5296)
                     /locus_tag="AC16G0_05710"
     CDS             complement(4367..5296)
                     /locus_tag="AC16G0_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099154.1"
                     /translation="MRRVAARAVWTAILLALGLLAAGSPARAAVATDMLGRSVSVPDA
                     PLRVVSLAPSLTEIVFALGRGDWLVGVTEFCDYPPEALSKPKVGGPVTPDLERILALR
                     PSLVLITEEGNPLQTVARLRQLGLPVFAVTPESFGGVLTSLLSLGRVLQAEAAAAKLV
                     QRIQARASAVQQAVQGRRRPRVLYLVWNEPLVAAGPASFVGDLLEMAGGENIVRERAV
                     AYPHLGWETVVGRAPEVVLVAEHRESDRSAVGAGPFRSNWSRWQSIPAVRSGRVLELP
                     SDMILRPGPRLGDGLEALARAIHPEAFGRAGSP"
     regulatory      complement(5426..5654)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5828..6169)
                     /locus_tag="AC16G0_05715"
     CDS             complement(5828..6169)
                     /locus_tag="AC16G0_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099155.1"
                     /translation="MARKLLLIGAVLVEYRVLSANPKGGESRMKKLSTLFLAAIFAVT
                     FSGYAFAQATPATPATPATPAAPAAPEKKAEKKTTEKKAAKKAPKKSAAKKGEKKGEK
                     KGEEKKEEKKS"
     gene            complement(6325..>7018)
                     /locus_tag="AC16G0_05720"
     CDS             complement(6325..>7018)
                     /locus_tag="AC16G0_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR5099156.1"
                     /translation="ARRGDLDRALAEVGRDLLARAERLSTARQRAARKLQEAILAELS
                     ELGMAKAAFQVQVARGALGEVGFGPHGIDEVEFLISPNPGESLKPLHRIASGGELSRI
                     MLALRTILATADQTPTLIFDEVDVGIGGSMAETVGRKLLAVSRQHQVLCVTHLPQIAC
                     HADHHVVVSKKTHRDRTETVVQALDDTDRPQELARMLGGPSRSGTGLQHAIELLEAAN
                     RLKRRMKSPQSK"
BASE COUNT         1208 a         2428 c         2224 g         1158 t
ORIGIN      
        1 cgcgaacagc ctgcggacac tgggggcgat cgacaggctg gctggcggtc gaggccgatg
       61 tggggcaagt ggcgggggga gaggcgacgg agcctcgcga acctccgtca gcagcttgac
      121 gcgaagactc gactcgcgag atgccggtgg ggggacgtgg aggtagagga tcccggcgag
      181 gagcaggccg tgcaagccca gcgagagtgc ggcccaacgc ccgagccatg ccgcctcgcc
      241 tgaccggtta ccaaacggcg cttgccgtcc cattgtcccc aggatctcca tcctcagaaa
      301 tcccccgaca tcccgatcag gtaccgccga cccagggcgg gaaatccgag cgcctcctca
      361 taccggtcgc ccaggatgtt ttccagcttg acgaaggccg tgagcctgcg gagcaggccg
      421 acctggtcca gcaccttcac cgctgcgccg gcattccaca gcacgtactt gaagttcgtg
      481 gtcgtcgcga aggtcgtggg atcgatgtca tttcgcttgc ccacgtagag gagtgtggag
      541 ttcaggttga tccgctccca gggtgcgtag ttcaccgcca cgttccaggt gtttgccggg
      601 cggcggagaa ggtgctggtt gttctgatcc agcgtctgga gataggtgta gctcccagcc
      661 agttccagcc cgtatcccgg acggaatccg agcgtcacct ccacgccttg cgtcctggcg
      721 tgggccacgt tcaccgggat gaacagcggg ccgctcgagg gctggatgag atcgcggaag
      781 cggctctgga accatgtcac gccggcctgg atccggtcgt gccagaggct ttgatccccg
      841 ccgaactcca ggctgcgatt cttctcgggc ttcagattgg gattggcaaa gcccggaaac
      901 gccaagtcgt tgatgcttgg cgcccggaat ccttccccgt aactcccgcg gagcttggac
      961 ccggtctccg ggacgagaaa cgccgccgag aatttcgggc tgacgtgcgt tccgaaggag
     1021 ctgttctcat cgacccgcac ccccgcggtg aggatcaatc gatcccacag cgtggcctgg
     1081 ttctgtccgt agaaggcgta gtcactgatc gccatgtgca cgacgcgggt cgcgaactgg
     1141 tccatctggt tcgccttggc atgatccacc tgaaagccca gggtgagcgt gttccaggag
     1201 accgggtgga tattgtgctg ccaatccgcc gccaggcggg tcgtctcggt gtcgaagggg
     1261 gtggagaacg caaggttggg tggagaagga aacgccgcca gtggatcgcg gaagtccacc
     1321 agatcctgga ccagcgagac ctgaaggcgg tgatcccacc aggatgttgg cgtgtgagtt
     1381 ccgcccaggg tcacgctggt cagatcctcg cggctgctct gggtgccgtc cggcagcccc
     1441 gtgttgaaat ccgtccgccc tggcaccgcc ggcaggttct tgaacgcgtg ggtgtaccgg
     1501 gcgctcaggt cgaagctgag attcgagagc gcttgataat ccaggcgggc ggcgctcgac
     1561 gtattgttgt actcgtcgtg gggaaacttc ccgctgctgt ccgttctcgc cacgtcgacg
     1621 gcatagccga cgggacccac cttgccgctg ctctgcccct gctcccgaaa tgtctggtac
     1681 gacccggcat ccacccggac ggtgccgcgg ggcgcaccat cgcccttccg cgtgacgatg
     1741 ttgatcacgg cggtgagccc gtccgagccg tacagggcgc tggccggccc acgcaccacc
     1801 tcgatccgct cgaccccctc ggtcgtcagg ttggcaaaat cgaagatccc gttggtcggg
     1861 ctgttcaccc gcacgccatc gatcatgacc agcgtgtggg aagagtttcc gccccgcgag
     1921 aagacactcg tcacagaacc cggggttccc gactggacca cgttcagcgc gggcacggtc
     1981 cgaagcgcct ccagaacggt ggtgatctgg cgccgcctga gctcctgctg cgtgatcacg
     2041 gtcacatccg ccgttgtctt ggacagggcc tcggggatcc gggccgccgt gaccacgacc
     2101 tcttccaagg tctgctccgc ctctggcggc tgttgctgtc cgccggccgt cgaggcccag
     2161 cccccagtgc cgaggagaat catcagtgcc caccagaggg atcgccgaca catgagccac
     2221 cttcctttcc gcgaagtggt gaggtctgcc cccggatagg tctcctgact tgtggatcat
     2281 ggcttccccg cgccttccca tcctccgaca cgcggaggac agtggcgatc agcggggtcg
     2341 ctccccacgt acagtggcgg gaccgtgctg gcttcccctc caggtccgga ccgaggcggt
     2401 cgggtctgag agctaccagc ttcccttaca tccggggggt ggtgagctgc ggaccgttgg
     2461 tgtgttcacg cggcaggctg ccgtggacgc gtggccggcc ggaagccggg tgccgctcca
     2521 cctccacggg gcagccgtat gccgacgtta aatgttcctc tgtgatcacc tcctcggggg
     2581 tgccgaaggc ccggacccgc ccttcgacca ggagcaagat ccggtcgcag tactcggccg
     2641 ccaggttcag atcgtgggag acaaggaccg cggtctgtct cccctcccga tggtgggcca
     2701 gcaggatctc caggacggtc cgctgatgcc caaggtccag gtgcgtcgtg ggttcatcca
     2761 agaggagaat cgaaggctcc tgcgccagcg cccgggcgat ggagacgagc tggcgctctc
     2821 ctccagacag ttcgtccacg cgtcggcctg ccagggcgag tatccccgtc gcctccatgg
     2881 ccgcttccgc gacggcgcga tccttgctgg tggcccagga gccgcccgcc gcgtgcgggt
     2941 agcggcccat caggaccacc tcggccacgc tgaagggaaa ggccacggag aactcttgcg
     3001 gaaccacggc caggctgcgg gccagttcga gtcgactcag ccgggcaaca tcccggccgc
     3061 cgatcaggat gcgaccgtgc tctgggacga cgaccttgct cagcaggcgc agcagcgtgc
     3121 tcttgccggc gctgttgggg cccacgaccc ccaagagatc ccccggcgcc gcctggaagg
     3181 tcacgtcctc gacgccctgc ccggtccggt accagaagcc gacgccctcg acctccagcg
     3241 ctttcatccg atccccctgc ccttcgccca tccctgtccc cctcgctgac cctgtcgccg
     3301 tcgcatcccc tggttcccgc tcacagcggg tcccctcccc gccgcgtccg cagcaggtac
     3361 acgaagagcg gcgccccgca gaaggcggtg atgaccccca ccggaagctc gctgggagcc
     3421 gccacggtcc gtgccaccag gtcggccagc atcaggaatg acgcccccag aagtgccgtc
     3481 gccgggacca gcctgcggtt gtcgggtccc aagagcaggc gcgccgcgtg cgggacgatc
     3541 aggcccacga atccgatgga tccggcggcc gagacggccg ctcccgtcac caaggaagca
     3601 gccacgaaga ccagacgctt gacccgctcc gcctccaccc cgagctggag cgcctgctcc
     3661 tcccccaaag ccagcaggtt caatcgcgcg gcctggcgcg tgaggacagc ggccccgatg
     3721 aggagcgcca gggccaggat cgccagggtc ctgacgcggg ccggtcccag gtttcccatg
     3781 agccagaaga ccaccccctg caattccgcc gatgccagga gcgccgtcgc cagcatcacg
     3841 caggcattga agaacaggcc gaccaccacc ccggagagaa gcagcgtggt gatggggagt
     3901 ctcccctcca cccgggcgat ggcataaaca gccaccgccg ccaggagggc cccgccgaac
     3961 gcgaagagcg tcaccacggc gggattcgcg aagggaccgg ccagacccag gagcacggcc
     4021 agcgtcacgc cgaaggatgc gccgctggag actcccagca ccgtcggatc ggccagaggg
     4081 ttacgggaga tcgcctgaaa ccccaggccg gccagggaca ggcaggcgcc cacgatccct
     4141 gccaggacgg cccgggggag gcggatctgc cttataaccg tcaccacagg gtcgtcggcc
     4201 cggaccccgc ttcccaagag cgcccgtccc acgtcgcgcg gagcgaccgg caggctgccg
     4261 accatcaccg atagcaggag ccccaggaga aggatcgcca gtagcaaggc aaaggccgcg
     4321 ggctgccagc ccataccctt gccgatgctc gccgagagcc ggaggctcac ggggaccccg
     4381 cccggccaaa ggcctccgga tggatggcac gggccaaggc ctccagaccg tcccccagcc
     4441 gcggccccgg tcgaaggatc atgtcgctgg gcagttccag gacccgaccc gatcgcaccg
     4501 cggggatgct ctgccacctg ctccagttcg accggaacgg ccccgcgcct accgcggacc
     4561 gatcagattc gcggtgttcg gccacgagaa ccacctccgg cgcccgaccc acgaccgttt
     4621 cccagccgag atggggatac gccacggccc gctccctgac gatgttctcg cctcccgcca
     4681 tctccaggag atcccccacg aagctcgcgg ggccggctgc caccaagggc tcattccaca
     4741 ccaggtagag cacgcgcggg cgcctccggc cctgcaccgc ctgctgcacc gcagacgccc
     4801 gagcctggat ccgctggacc agcttggccg cggccgcttc ggcctggagc acgcgaccca
     4861 ggctcaggag ggatgtgaga accccgccaa agctctcggg cgtcaccgca aagacgggga
     4921 ggccgagttg cctcagacgg gccacggtct ggaggggatt cccttcttcg gtgatcagga
     4981 cgaggctcgg acgaagcgcg aggatgcgct ccagatccgg ggtcacgggc ccgccaactt
     5041 ttggcttgga caaggcctcc ggcgggtaat cgcaaaactc ggtgacgccg acaagccagt
     5101 cccctcgacc caaggcgaag acgatctcgg tgaggctcgg cgccaacgac accacgcgca
     5161 ggggcgcgtc cgggacgctg acagagcgtc ccagcatgtc ggtggccact gccgctcggg
     5221 ccggcgaccc ggccgccaga agacccagcg ccaggagtat ggccgtccac acggccctgg
     5281 cggctaccct gcgcatggct ctttccacca gaaacacgaa agccccgatc cgcgcgtagc
     5341 gggtcggggc tccaaaatcc aaccgtccgg acgtctcaac cagggaccgg tagatggtgg
     5401 gctgcccctc cccgcggaag gatgactaca cccagaaggc cgagcaggtt tcctggctcg
     5461 cggctcagct tactctctgc gccttcccgt tccgcgccgc gcgggacagt ggccgtgcag
     5521 atttcaacgc cgctcacagt tgcggggcag caatggattc tccctcgcgc catccgggag
     5581 gacggagtgg gagggatgca ccattttccc ttggggtccc ctcgggaggg accactcggc
     5641 ttcgcctatt caattgtccc gactagacct cctctgaaca agccgatgac aacggacttt
     5701 taatcgaagc agcggatgaa gtcaaggcat tttcatgcca gtcccgggga tcccccccag
     5761 aaaagcaaga gggcatccgg ggttcggatg ccctcaagct gagtcgattg tcgaatagag
     5821 agaaggacta gctcttcttc tcttccttct tctcttcgcc cttcttctcg cccttcttct
     5881 cgcccttctt cgcagcagac ttcttcgggg ccttctttgc cgccttcttc tcggtggtct
     5941 tcttctccgc cttcttctca ggagccgccg gagcagccgg agtcgccggg gtggccggag
     6001 tcgccggggt ggcctgcgcg aaagcgtagc cggagaaggt caccgcgaag atagccgcca
     6061 ggaacaacgt ggaaagcttc ttcattctag attcacctcc cttcggattg gcactcagaa
     6121 ccctatactc gaccaggact gctccgatca gaagcagctt gcgtgccaac attgcctttc
     6181 cgcggtagat atttctcgct ggattttcaa tgacttagag ggaggattga tctaggaaac
     6241 ttagaggcat ttgtcagcca agctttggct gcatcctttg ggttggtcat gcaccttttg
     6301 gagcaatctc ccctttccga cggctcattt cgactgaggg gatttcatcc gccgcttgag
     6361 gcgattggcc gcctcgagca gttcaatggc gtgctggagc cccgtgccgg atcgacttgg
     6421 cccccccagc atccgggcca gctcctgggg gcgatccgta tcatccagcg cctgcaccac
     6481 ggtctccgtc cgatcccggt gggtcttctt ggagacaacc acgtggtgat cggcatggca
     6541 cgcgatctgg ggaaggtgcg tgacgcacag gacctgatgc tggcgcgaga ccgccagcaa
     6601 ctttcgcccc acggtctcgg ccatgctgcc gccgatcccg acatccacct cgtcgaaaat
     6661 caacgtcggc gtttgatccg ccgtggccag aatcgtccgg agggccagca tgatgcgcga
     6721 tagctccccg cccgacgcga tccggtggag cggcttcagg gactccccgg ggttcgggga
     6781 gatgagaaac tccacctcgt ctattccgtg cgggccgaac cccacctccc ccagcgcccc
     6841 ccgggccacc tggacctgga aggccgcctt ggccattcca agctcagaca gctccgccag
     6901 gatcgcctcc tggagcttgc gcgccgctcg ctgacgcgcc gtcgagagcc gctcggcccg
     6961 ggcgaggaga tcccgcccga cctcggccag tgcccggtcg agatcgccac gccgggcc
//
LOCUS       DBNGZC010000315         2270 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2275810, whole genome shotgun sequence.
ACCESSION   DBNGZC010000315 DBNGZC010000000
VERSION     DBNGZC010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2275810"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="AC16G0_05725"
     CDS             <1..395
                     /locus_tag="AC16G0_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR5099157.1"
                     /translation="APPAPPVASVGTGAATGAAKGSGVELAGEGAGAGGGTALAAYLS
                     LVDWKIQSNWVPVPIAGVPEIVVMVRFRVLRSGQVRDVELDASSGNASVDTSALRAIR
                     QSMPLPPFPNLLTEPSLDLRYRFVMERG"
     gene            395..1708
                     /gene="tolB"
                     /locus_tag="AC16G0_05730"
     CDS             395..1708
                     /gene="tolB"
                     /locus_tag="AC16G0_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HIR5099158.1"
                     /translation="MHNHHWVAFGLCLSLLGGLAPGAEGQVIQRELRERQVSKITIAI
                     AGFPSFSLNNQDLGKLGASILANDLRFTTIFDVMDPATLPFDPATVQLDQERPLFPGL
                     NSLKVQNLAVANVSSRGREAVVEGRLFDVANGNMIFGKRYVGDSKFYRLIIHRFADDI
                     MFNLTGEKGVAQTRIAYVAAVSRTEKEIYVMDYDGANPTPITGNQSLNVSPRWSPDGR
                     LIAYTSYRSGKPDIFVLNFDSGRRDLLPAIATLKATSAWSPGWSPDGQWLIAALGVGS
                     GTNLYLIPRGGGTAKPLTSGSAISVSPSFSPNGRQIVFNSDRGGTPQIYVMDVEGTNV
                     RRLTFQGNYNASPRWSPRGDKIVFMCRAGGNQICLINPDGTGLQQLTTQGNNEDPAWS
                     PDGRHVVFSSTRTGLRDIFVMHADGSEQTRLTNGGRDSYLPDWSP"
     gene            1756..>2270
                     /locus_tag="AC16G0_05735"
     CDS             1756..>2270
                     /locus_tag="AC16G0_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099159.1"
                     /translation="MRYIISAPVLLAAIVLLGGCVTSSDIDAVRRDIAGVRQEVAAQA
                     KTSEGARAFTENRLSKLEKDFRSQFESSVKESEGSRVALNQRLEELTTETRFVQGKLE
                     ENASVLKDLNTRLDDMDQKIGQSNRRVDALDQQVKALGQQVRTLDQQIRGPAPPASPV
                     VPAPGAAPGQMP"
BASE COUNT          407 a          740 c          729 g          394 t
ORIGIN      
        1 tggcaccgcc ggcgcccccc gtggcatccg tgggaaccgg agccgccacc ggcgccgcga
       61 agggcagcgg cgtcgagctt gccggggaag gggcaggggc ggggggcgga acggccttgg
      121 ctgcctatct ctccctggtg gactggaaga ttcagagcaa ctgggtgccc gtcccgatcg
      181 cgggggtgcc tgagatcgta gtgatggttc gtttccgggt gctgcggtcg ggccaggtgc
      241 gtgatgtgga gttggatgcc agttcgggga acgccagcgt ggatacctcc gcgctccggg
      301 ctatccggca gagcatgccg ctgcccccct tccccaatct tctcaccgag ccctctctgg
      361 accttcgcta ccgctttgtc atggagcgcg gctaatgcat aatcaccact gggtggcttt
      421 cggactctgc ctgtccctcc tgggaggact cgctccagga gccgagggac aggtgatcca
      481 gcgggaactc cgcgagcgac aggtgtcgaa gatcaccatc gccatcgcgg gattcccctc
      541 gttctcgctg aacaaccagg acctggggaa gctgggtgcg tcgatcttgg ccaacgacct
      601 caggtttacc accatcttcg acgtcatgga tccggcgacc ctacccttcg atcccgcaac
      661 ggtccagctg gatcaggaac gtccgctctt ccccggcctc aattccctca aagttcagaa
      721 cctcgcggtg gcgaatgtca gcagccgggg gcgcgaggcc gtcgtggagg ggcgcctgtt
      781 tgacgtggcc aacgggaaca tgatcttcgg caagcgctac gttggagatt ccaagttcta
      841 ccggctgatc atccatcgtt tcgccgacga catcatgttc aatctgaccg gggagaaagg
      901 ggtggcccag acgcggattg cctacgtcgc cgccgtaagc cggacggaga aggagatcta
      961 cgtcatggac tacgacgggg cgaatcccac acccatcacg ggcaaccagt ccctcaatgt
     1021 ttcgccgcga tggtccccgg acggacgcct gatcgcctac acctcctacc ggagcggcaa
     1081 gccggacatc ttcgtcctca acttcgatag cggccggcgg gatctcctcc cggcgatcgc
     1141 gacgctgaag gccacctccg cctggtcccc cggctggtcc cccgacgggc aatggctgat
     1201 tgccgctctg ggggtcggca gcgggaccaa tctgtatctc atcccaaggg gcgggggcac
     1261 ggcgaaaccg ctgaccagtg gctcagccat ctcggtctca ccgtcctttt cccccaacgg
     1321 ccggcagatc gtcttcaact cggaccgcgg ggggacaccc cagatctacg tcatggatgt
     1381 ggaggggacc aacgtccgcc ggttgacgtt ccaaggaaac tacaatgcct cgccccgatg
     1441 gtctccccgc ggggacaaga tcgtcttcat gtgccgggcg gggggaaacc agatctgtct
     1501 catcaatccc gacggtaccg gcctgcagca gctcaccacc cagggaaaca atgaggaccc
     1561 cgcctggtcg cccgacggcc ggcacgtcgt tttctcctcg acccgcacgg gtcttcggga
     1621 tatcttcgtg atgcacgccg acggctccga gcagacgcga ctcacgaacg gcgggcggga
     1681 cagttatcta cctgactggt cgccctaggc tggagaatcc ctcattcccc gacctcggaa
     1741 ggaggtggaa gtccattgcg ctacatcatc tcggcacccg tcctcctggc cgccatcgtg
     1801 ctgctggggg ggtgcgtcac gagttcggac atcgatgcag tccgccggga catcgccggt
     1861 gtccgacagg aggtggcggc ccaggccaag accagcgaag gggctcgggc cttcaccgaa
     1921 aatcgcctga gcaagctgga gaaggatttc cggagccaat tcgagtccag cgtgaaggag
     1981 agcgagggct cccgcgtggc cctgaatcag cgcctggaag agctcacgac ggagacccgc
     2041 ttcgttcagg gaaagctgga ggagaacgcc tccgtcctca aggacctcaa cacccggctg
     2101 gatgatatgg atcagaagat cgggcagtcg aatcgtcgcg tggacgcctt ggatcagcag
     2161 gtgaaggcgc tcggccagca ggtgcggacg ctggatcagc agatccgggg ccccgccccg
     2221 ccggcatccc cggtggtgcc tgcgcccggt gcggctccgg ggcagatgcc
//
LOCUS       DBNGZC010000316         7320 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1100142, whole genome shotgun sequence.
ACCESSION   DBNGZC010000316 DBNGZC010000000
VERSION     DBNGZC010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7320)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7320
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1100142"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(527..1198)
                     /locus_tag="AC16G0_05740"
     CDS             complement(527..1198)
                     /locus_tag="AC16G0_05740"
                     /inference="COORDINATES: protein motif:HMM:NF014543.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HIR5099160.1"
                     /translation="MRCLLATAVVAIAVMLPLPVAADDGRWRPTISNGCLVWDALPQP
                     DETVTWSGACVDGKASGRGTEVFRYRDHGVWKEERYVGDMRGGKLNGRGTLTYDNGDR
                     YEGDFVDAKRVGHGTYTHANGDRYEGDFREDQRTGRGSFTYHNGGRYEGEFVNGRFQG
                     HGTFTFADGGRYEGEFKGGMANGDGTYTSSTGEVVTGNWSNGCIQQGGRVAAVGTTKE
                     KCRFR"
     gene            complement(1269..2267)
                     /locus_tag="AC16G0_05745"
     CDS             complement(1269..2267)
                     /locus_tag="AC16G0_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013087.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HIR5099161.1"
                     /translation="MAEPWTAEALLETVRGYHPACVLIAGAELDVFTILHEQPMGASS
                     LATRIQSDPRAMAILLDALAAMRLLVKEEGAYHVPAAVADLLTETGTRCQLGMIRHQG
                     NCLRRWGQLARVVRTGRPITGEVSIRGELADRASFIRAMHEISDPVAAPLLASLEPLV
                     FRNLLDLGGASGTYTIPFLRLHPAATATIFDLPEVLPMAEPLLKAAGLANRVRLAAGN
                     FDTDALPTGADLAWVSAIVHQNSREQNRALFGKVFAAMVPGGRILIRDFVMDEARTTP
                     PAGAFFAVNMLVATPGGGTFTLPELQADLGAAGFVDPRLLRRGEGMDSVVSAIKPG"
     gene            2834..4099
                     /locus_tag="AC16G0_05750"
     CDS             2834..4099
                     /locus_tag="AC16G0_05750"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099162.1"
                     /translation="MKRSPGNPISRRTFVKATAGAALISATGPFVLRHALGADPLVIG
                     VVSPASGNYADHGMMERLGMTMAAEEYKEKGVLGRPVKLVVEDDETDPQVGARKARRL
                     IEVDKVKYLIGGVSSSVAVSVGEVAQRNGAIFIGSNQNSDTLTEQYGRRVQFMVPPDM
                     AMALRTLAPYLIEKVGKRWYFLTHDYEWGWSGTKWAKIILQKFGGTALGESKIPMNPR
                     DFSAFLLKVRSAKPDALVLTVGGIDLAALMEQMYEFGIYKETTVVSTLINFEDLWAVG
                     PDKMQGIHLMEWHHYIDAPGVKEFVAAYKRRWPATLIPVPTANTVNGYIGVRELLRGI
                     ERAGKDDVTAVIKAMEGHTSHDSLKNDPLTIRDWDHKFLWASYVVRSKKGNEMKDRTD
                     FVEILRWAPGKETARTRQENKVNLGPYPS"
     gene            4185..5054
                     /locus_tag="AC16G0_05755"
     CDS             4185..5054
                     /locus_tag="AC16G0_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5099163.1"
                     /translation="MMELLSFTMNGLVLGLIFAVVAMGFTLILGVMEVINFAHGILFA
                     LGAYFAFSMQRYVGFWLALILAPLLVGLVGLVLERLVVHRTYGGNPLFGLLVTFGLAT
                     GLEELIRMIWGKTGYSVAAPKFAAGSLDLGFMIYSKYRLVLAALAILIILLVWLFLEK
                     TPYGAIIRAGASDAEMVMALGKNLPLMRTFVFIIGSILAGVAGVIAAPVWSVRPTMGT
                     AILMPAMIIVVLGGVGSFWGSVLAGLIVGLSTSISIMFWARISDIIPFVILMLVLLFR
                     PRGLMGEKSVLEV"
     gene            5057..6031
                     /locus_tag="AC16G0_05760"
     CDS             5057..6031
                     /locus_tag="AC16G0_05760"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5099164.1"
                     /translation="MGSAPNRRGWAASPMVWVILGFAVFPFIPFVGRYLSLATEVVIW
                     ALFALGFNILLGYTGLPSFGHGAFFGIGAYAAAMWFLRLSPGVWGPLMAAVVAAGISA
                     VLIGGLVAKKRGIYFALITIAVSQVFYFIAFRWDEVTGGETGLSGIDRLNVGVPGLLT
                     LKLANGLVYYYFVLAIFVLCMLLIWRIVHSPFGRVLQAIRENEVRASYLGYPTALYKW
                     GAFAVSGLFSGLAGGLYTLLINAAFANILDWTQSGNVVMMTLLGGGTVNFFGPILGAA
                     IFIVSRDLLSAYWESWMLAYGMIFVLVILFIPSGILGLVGGKKQTILR"
     gene            6041..6793
                     /locus_tag="AC16G0_05765"
     CDS             6041..6793
                     /locus_tag="AC16G0_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099165.1"
                     /translation="MAILTTEGVTKNFGGLRAVDGLDLVVEEGKLTSVIGPNGAGKTT
                     LFNLITGMIRPDAGRVLFRGEDITHLPIYQIVRRGISRSFQILNLFNELTLLENVWLG
                     VQAEQGHGPELLVHADALTSVRDETERLIADVGLASKAELPVKLLSYGDRRLLEITLS
                     LTTRPALLLLDEPMSGLTSEDRKRVADFMRALAGRITLLVVEHAMDVVLSISDHIVVM
                     HQGRLLAQGTPDAIRANAGVQEAYLGGQYAAV"
     gene            6813..>7320
                     /locus_tag="AC16G0_05770"
     CDS             6813..>7320
                     /locus_tag="AC16G0_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099166.1"
                     /translation="MLAVEKIQTFYEDAQALFDVSLTVEAGEVVALLGRNGAGKTTTI
                     RSIMGLTPPRAGRIMFQGRDIVSLPAYSIANLGVGFVPDNRRIFPTLTVKQNLELARK
                     RGNRGHNGWTLESIYHHFPKLKEIENRKGEVLSGGEQQMLTIARTLMGNPDLVLLDEP
                     TEGLSPLMV"
BASE COUNT         1203 a         2429 c         2341 g         1347 t
ORIGIN      
        1 gtagaggctg ggagggccaa tcccgagacg cggttgacaa ccatcagcac gaagccgacc
       61 accagcaggc gtcggcgggg acggatcagc tccgcgatca agggccaaag cgcccgaagc
      121 cgctcaggcg ccgctgtgtg ttttccgtgc gcgacagtgt ccggtggtat ccgcctggag
      181 ctcatgtgtg aacctcggta gtcagtcatc tggcgaggag tgggacgacc acggccatcc
      241 cccatttcgc ccaagacgat gtccggctgc gaccccagta ggactgcgcg tcattcccgc
      301 cggacgcgag gcgggttctg ggcgacgcca ctctaggcgg gcagtgagca cccgtcaaca
      361 cgctacaggc gccggtgagc catgagacgc tcgcgcggag cagcgtccat tcggttccct
      421 cggggcgtcc aggcagtctc cggttcgctc ggagcacacc cctcagcctg tgatgcgcgg
      481 gccgagatga gtggatgggc ttcttcgccg acgattgtct cgactcttat ctgaacctgc
      541 atttctcctt agtggttccg acggccgcca cccggccgcc ttgttggata catccgttcg
      601 accagttgcc ggtaacgacc tccccggtgc tggatgtgta ggtgccgtcg ccgttggcca
      661 tgccgccctt gaactcgccc tcgtagcggc cgccgtcagc gaaggtgaac gtcccatggc
      721 cttggaagcg accattgacg aactcgccct cgtagcggcc gccgttgtgg taagtgaacg
      781 acccgcgtcc cgtgcgctgg tcttccctga agtcgccctc gtagcggtcg ccgttcgcgt
      841 gggtgtacgt tccgtgtccg acccgcttgg cgtccacgaa gtcgccctcg tagcggtcgc
      901 cgttatcgta ggtgagcgtt cctcgtccgt tgagcttgcc gccccgcata tcaccgacgt
      961 agcgctcttc cttccacacg ccgtggtcgc ggtaacgaaa gacctcggtg ccgcgcccgc
     1021 ttgcctttcc atcgacgcaa gcgcccgacc acgtgacggt ctcgtcgggc tgtggcagcg
     1081 cgtcccacac aaggcagccg ttgctgatgg tcggccgcca gcgcccatcg tccgccgcca
     1141 ccggtagcgg cagcatgacg gcaatggcaa caacggcagt ggcaagcaag cagcgcatgc
     1201 gcagaccctc cgcggaggaa cccatagagc gcttccttga gctgtgtctc aagctccgaa
     1261 gtccgggcct accccggttt gatcgcactg acgaccgagt ccattccctc accgcggcgt
     1321 aggagtcggg ggtcgacgaa gccggcggcg ccgaggtccg cttgcaattc gggtagcgtg
     1381 aacgtcccac ctcccggggt cgcaaccagc atgttcacgg cgaagaaggc gcccgccggg
     1441 ggggtcgtgc gggcctcgtc catgacgaag tcgcggatca ggatccggcc tccggggacc
     1501 atggccgcga agaccttccc gaacagcgcc cggttctgct cgcgcgagtt ctgatggacg
     1561 atagcgctca cccaggcgag atcggccccg gtcgggagcg cgtcggtgtc gaagttcccc
     1621 gccgccagcc gaacgcggtt tgccaggccg gccgctttca agagcggctc ggccatggga
     1681 aggacctccg gcaggtcgaa tatcgtggcc gtggcagcgg gatggagccg caggaagggg
     1741 atcgtgtagg tgcccgatgc cccgccgagg tccaggaggt tccggaacac caggggctcc
     1801 aggctcgcga ggagcggcgc cgcgacgggg tcggagatct cgtgcatcgc ccggatgaag
     1861 gaggcccggt ccgccagttc tccccggatg ctcacctcgc ctgtgatcgg ccggccggtc
     1921 cggaccaccc gggccagttg tccccagcgg cgcaggcagt ttccctggtg gcggatcatg
     1981 ccgagctggc agcgcgtgcc tgtctcggtg agcaggtccg caacggcagc aggcacgtga
     2041 tacgccccct cttccttgac gaggagacgc atcgcggcca gagcgtccag caggatcgcc
     2101 atggcgcggg ggtcagactg aatccgggtc gccaggcttg acgcgcccat tggctgctcg
     2161 tgcagaatgg tgaacacgtc gagctctgcc cctgcgatca agacgcaggc cggatgatac
     2221 cccctcaccg tctccaagag ggcttcggct gtccacggct cggccattgt ccgcctcctt
     2281 tctgttccgt cggcaacccc gcgtgcaaac cggggcggca acgaatcgga ccacgggaca
     2341 tcgtgagccc ggcgggtttc cctggcgatc gccagccggg aaaatcatcc tatcggatca
     2401 ccggatttta gcaagccccg caacatctag cccacaatcc cggacagccc cgccgccctc
     2461 gcctccattc ggccgcctgc ccggggtaca cttctcagca ctggctcttc cctgcttcga
     2521 acggattcac ctccgctggc ttgaaggcag tgcctgttgt cgaagcactc ggcccgcagg
     2581 ttgaggacct ccccatcaac ccacgccagg gacaggacgg cagcatttcc agccgacaaa
     2641 gatagccata cgatgggtcg tccgaacaaa cccaaatcgc cagggattcc gtccgctgga
     2701 aaacttgaca agcctttcgc ttcccatatg atgggggcca tttattctcg gatcagtcag
     2761 ggccattggg gaaaggaggc agagcacttt cttcattcga ctccgggatc atctcttaca
     2821 ggaggaccag gcaatgaaga gaagtccagg caacccgatt tcccgcagga cgttcgtcaa
     2881 ggccacggcc ggcgcggccc ttatcagcgc tacaggcccc ttcgtgctcc gccacgctct
     2941 gggtgccgat ccccttgtca tcggcgtcgt cagtcccgcc agcggcaact acgccgacca
     3001 cgggatgatg gagcgcctcg gcatgaccat ggcggccgag gagtacaagg agaagggcgt
     3061 cctgggtcgc cccgtgaaac tcgtcgtgga agacgacgag accgacccgc aggtcggggc
     3121 gcggaaggcc cggcggctga tcgaggtcga caaggtcaag tacctgatcg gcggtgtctc
     3181 cagttccgtc gccgtctccg tcggcgaggt ggcacagcgg aacggcgcca tcttcatcgg
     3241 ttccaaccag aattccgaca ctctgaccga gcagtacggc cgccgtgtcc agttcatggt
     3301 tcctcccgac atggccatgg cgctccgcac gctggctcca tacctcatcg agaaggtggg
     3361 gaagcgctgg tacttcctca cccacgatta tgagtggggc tggtccggca cgaagtgggc
     3421 gaagatcatc ctgcaaaagt tcggcggcac cgccctgggc gagtccaaga tcccgatgaa
     3481 cccccgggac ttcagcgcct tcctgctcaa ggttcgctcg gccaagccgg acgctctggt
     3541 gctcaccgtg ggcgggatcg atctggccgc gctcatggag cagatgtacg agttcgggat
     3601 ctacaaggag accaccgtcg tgtccacgct catcaacttc gaggacctgt gggcggtcgg
     3661 gcccgacaag atgcagggta tccacctcat ggagtggcac cactacatcg atgcgccggg
     3721 ggtcaaggag ttcgtggccg cctacaagag gcgctggccg gccaccctca tccccgtgcc
     3781 gaccgcgaat accgtgaacg gttacatcgg cgtgcgcgag ctgctccgcg gaatcgagcg
     3841 cgcgggcaag gacgacgtca cggccgtcat caaggcgatg gagggtcaca cctcccacga
     3901 ctccctcaag aacgaccccc tcaccatccg cgactgggac cacaagttcc tctgggcctc
     3961 ctacgtggtc cgctcgaaga agggcaacga gatgaaggac cgcaccgact tcgtggagat
     4021 cctgcgctgg gccccgggca aggagacggc ccggactcgc caggagaaca aagtcaacct
     4081 cggcccctat ccgagctagt gctgagtcgg gaggggggcg caggcgcccc cctcccgcat
     4141 gcgtcccagc gtcgcctcgc ttacccgtcg gacggagcgt ccccatgatg gaactcctgt
     4201 cgtttaccat gaacggcctc gtgctgggtc tgatcttcgc cgtggtcgcc atgggattca
     4261 ccctgatcct cggcgtgatg gaggtcatca acttcgcgca cgggattctg tttgccctcg
     4321 gcgcctactt cgccttctcc atgcagcggt acgtcggctt ctggctggcc ctcatcctcg
     4381 cgccgctcct ggtcgggctg gtcggcctgg tcctggagcg gctggtcgtt catcgtacct
     4441 acggtggcaa cccgctcttc ggcctgctgg tcaccttcgg tttggcgact ggccttgaag
     4501 agttgatccg gatgatctgg ggaaagaccg gctactcggt cgccgccccc aagtttgccg
     4561 ccggctctct cgacctcgga ttcatgatct actcgaaata ccgtctcgtc ctcgccgccc
     4621 tggccatcct catcatcctc ctggtttggc tcttcctgga gaagacgccc tacggcgcga
     4681 tcatccgcgc gggggcgagc gacgccgaga tggtgatggc gctggggaag aacctccccc
     4741 tgatgcggac cttcgtgttc atcatcggct cgattctcgc cggcgtggcc ggggtgatcg
     4801 cggcgccggt ctggagcgtc cggccgacca tgggcaccgc catcctgatg ccggcgatga
     4861 tcatcgtggt cctgggcggg gtcggaagct tctggggcag cgtcctggcc ggcctgatcg
     4921 tcggcctttc gaccagcatc tccatcatgt tctgggcccg gatctcggac atcatcccct
     4981 tcgtcatcct gatgctcgtc ctcctgttcc ggccgcgcgg actgatgggc gagaagagcg
     5041 tcttggaggt gtgagcatgg gatcggcacc gaatcggcgc ggctgggcgg caagccccat
     5101 ggtctgggtc atcctgggat tcgccgtgtt cccgttcatc cccttcgtgg gacggtacct
     5161 gtcgctggcc accgaggtcg tgatctgggc gctctttgcc ctgggcttca atatcctgct
     5221 gggctacacc gggctaccct ccttcggcca cggggcgttc ttcgggatcg gcgcgtatgc
     5281 ggccgccatg tggttcctgc gcctctcccc cggtgtctgg ggaccgctca tggcggccgt
     5341 ggtcgctgcc ggcatctcgg cggtcctgat cggcgggctg gtggccaaga agcgcgggat
     5401 ctacttcgcg ctcatcacca ttgccgtgag ccaggtcttc tacttcatcg ccttccgctg
     5461 ggacgaggtc accggcggcg agaccggcct gtccgggatc gaccggctga acgtgggggt
     5521 ccccggcctg ctcacgctca agctggcgaa cgggctcgtg tactactact tcgtcctggc
     5581 gatcttcgtc ctgtgcatgc tgctgatttg gcggatcgtt cactctccct tcggccgcgt
     5641 gctgcaggcc atccgcgaga acgaggtgcg ggcgagctac ctgggctacc ccacggcgct
     5701 gtacaagtgg ggtgccttcg ccgtgtcggg cctcttctcg gggctcgccg gagggctgta
     5761 tacgctcctc atcaacgcgg ccttcgcgaa catcctggac tggacccagt cgggcaacgt
     5821 cgtgatgatg accttgctcg gcggcggaac ggtcaacttc ttcggtccga tcctgggggc
     5881 cgcaatcttc atcgtctcgc gggacctgct ctccgcctat tgggagtcct ggatgcttgc
     5941 ctatggcatg atcttcgtcc tggtgattct gttcatcccg agcggaatcc tgggactggt
     6001 cgggggaaag aagcagacca tcctgcgcta gggagattcc atggccatcc tgacgaccga
     6061 gggcgtgacg aagaatttcg gggggctgcg ggctgtggac gggctcgatc tggtggtcga
     6121 ggagggaaag ctaacctcgg tcatcggtcc caacggcgcg ggaaaaacca cgctcttcaa
     6181 cctgatcacc ggcatgatcc ggccggacgc gggacgggtg ctgttccgcg gcgaggacat
     6241 cacccacctc cccatctacc agatcgtgcg gcgaggcatc tctcgctcct tccagattct
     6301 caatctgttc aacgagctga ccctcctgga gaacgtgtgg ctgggcgtgc aggcggagca
     6361 ggggcacggg ccggagctcc tggtccacgc ggatgccctc accagcgtcc gggatgagac
     6421 tgagcgattg atcgcggatg tcggcctcgc ctccaaggcc gagctgccgg tcaagcttct
     6481 ctcctatggc gatcgccggc tgctggagat caccctctcc ctcacgaccc ggccggcgct
     6541 cctcttgctg gacgagccga tgagcggtct cacgtcggag gaccgcaagc gagtcgccga
     6601 tttcatgcgt gccctcgccg gccgcatcac gctgctcgtc gtcgagcacg ccatggatgt
     6661 cgtcctctcg atctccgacc acatcgtggt catgcatcag gggcgcctgc tggcgcaggg
     6721 aacgccggac gcgatccgcg caaacgcggg agtccaggag gcctatctgg gcgggcagta
     6781 cgctgccgtc taaccaggcg acggagggcg cgatgctggc ggtggagaag atacagacct
     6841 tttacgagga cgcccaggct ctgttcgacg tctcgctcac ggtcgaggcg ggcgaggtcg
     6901 tcgccctgct gggccggaac ggggccggca agacgactac catccgttcc atcatggggc
     6961 tcaccccgcc gcgcgcgggg cgaatcatgt tccagggccg ggacatcgtg agccttccgg
     7021 cgtactcgat cgccaacctc ggggtcggct tcgtccccga caaccggcgc atcttcccca
     7081 ccctgacggt gaagcagaac ctggaactgg ccaggaagcg tggcaatcgc ggccacaatg
     7141 gctggacgct ggagagcatc tatcaccatt tccccaagct gaaggaaatc gaaaaccgca
     7201 agggcgaggt gctgagcggc ggcgagcagc agatgctcac catcgcgcgc acgctgatgg
     7261 gcaacccgga cctggtgctg ctggacgagc cgaccgaggg cctctcgccg ctgatggtga
//
LOCUS       DBNGZC010000317         3641 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_221078, whole genome shotgun sequence.
ACCESSION   DBNGZC010000317 DBNGZC010000000
VERSION     DBNGZC010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_221078"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2388)
                     /locus_tag="AC16G0_05775"
     CDS             complement(<1..2388)
                     /locus_tag="AC16G0_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM0106 family RecB-like putative nuclease"
                     /protein_id="HIR5099167.1"
                     /translation="MATKVTRDVLEGYLHCKTKAHLKLAGQQGSASDYEKLLVASRGE
                     VRNTAIGKILERYPESEVASGIPLTASALRAGPSFVLDATLEDDLVSLRFDGLKRADG
                     PSKLGDFHYVPMLFHEGRKVDKEQRLLLELFGLLLSQVQERLPASGVVWHGRECRASK
                     IRLNPDVRRTERLLQEVREMIGAESPPRLILNDHCRVCEFRRVCQATAVEKDDLSLLR
                     GLSPKDIAGLNRRGIFTVTQYSYTFRPGRMRRAVEWKSPKHDHSLQALAVRDNTVYVA
                     QKLELPDGKARLYLDVEGLPDADSYYLIGLTVEEGSTRRRLCFWADHGAEEASMWRSF
                     LDVAGPIEDFVLFHYGSYESHFLGKMKARHGGDPELIAKLKSRSVNVLSLIYGRVYFP
                     VHSNDLKSVAGCLGCCWSAADASGLQSIVWRHAWETTRDEVVKQRLLAYNEEDRLALA
                     TVVELLCFVSSESQPGDIGTGPRVAAVEDIEVPRRHKFCDPEYVLPEFGRITKCAYFD
                     YQRDKVLFRTNPAMRRSIRCKKQQERRTLKVDREIDCGLPTVCPYCGSTRLTPHYRHQ
                     KTVFDLKPSRAGIKRWVTRYKARRCRCGACGHTSLPAEYLAVSAYKYGPSLCSWVVYS
                     SISQRQTSESVAEALNDLFAVRIPLGSLTDLVQRAAERYRPTYEALIACLRTGQLVHA
                     DETKTMIKGSSRSHYVWAFVNPQTVIYVYSTTRDGDTPRDTLAGFRGVLVSDFYAAYD
                     SLDCPQQKCLIHLVRDLNDDLLKHPFDAELKQMTARFAGLLQAVVETIDRYGLKKY"
     gene            complement(2596..>3641)
                     /gene="tnpC"
                     /locus_tag="AC16G0_05780"
     CDS             complement(2596..>3641)
                     /gene="tnpC"
                     /locus_tag="AC16G0_05780"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HIR5099168.1"
                     /translation="DKPPQPIDKGMPGPGLMAQTITSKYSDHLPLYRQEDILARHGVV
                     LSRSTLCGWMAQAADRLAPLYDLMVQRVLWSTIIHTDDTPVPVWDPTLPKTRTGRVWV
                     YVGDLRYPYVVYDYTPRRTRDGPEQFLKGFRGYLQADAFSGYDRMCAGPDVIEVACWA
                     HVRRKFFEARTSALVPAHAALGRIRQLYAIEHAAEDSSAQDRYALRQRDSVPLLVAFG
                     EWLTEQGRHVLPKSPIGQAIAYTQSNWAALCRYPEHGELSIDNNLAERLLRAQAIGRR
                     NWTFLGSDRGGRTAAILYSLTGSCKHHDIDPFAYLDDILRRLPSQPAGRLDELLPDVW
                     FASHPSARRKTAA"
BASE COUNT          649 a         1168 c         1131 g          693 t
ORIGIN      
        1 gtattttttc aggccgtagc ggtcgatagt ctccaccacc gcttgcagca atcccgcaaa
       61 ccgggcggtc atctgcttca gttcggcgtc gaacgggtgc ttgagcaggt catcgttcaa
      121 atctcgcacc aaatgtatga ggcacttctg ttgcgggcag tccaaagagt cgtatgcagc
      181 gtagaaatct gataccagga cgccacggaa cccggccagc gtatccctcg gcgtatcccc
      241 gtctcgcgtc gtggaataga catagattac agtttgcgga ttgacgaagg cccacacgta
      301 gtggctccta ctactccctt ttatcatcgt cttggtctca tcggcgtgga ccagttgtcc
      361 ggtccggagg caggcaatga gggcctcgta ggtcggccgg tagcgctctg ctgctcgctg
      421 cacgagatcg gtcagtgagc ctagcgggat gcggacggcg aacaggtcgt tgagcgcctc
      481 ggccacagac tcgctcgtct gccgctggga gatggaagaa tacaccaccc agctacacaa
      541 gctcggcccg tacttgtagg cagagacggc cagatattcg gccgggagcg aggtatgccc
      601 acaggctcca caccgacagc gtctagcttt gtagcgtgtt acccacctct taatgcccgc
      661 gcgggaaggt ttgaggtcga acacggtttt ttggtgccga tagtgtgggg tcaggcgggt
      721 tgagccgcaa taggggcaga ccgtaggaag tccgcagtca atctcgcggt ccaccttcaa
      781 agtccgccgc tcctgctgtt ttttgcagcg tatgctcctt ctcatcgcgg ggttcgtgcg
      841 gaagagcacc ttgtcccgtt ggtagtcgaa gtaagcgcac tttgtaatcc gaccgaactc
      901 gggaagaacg tactccggat cgcagaactt gtgtcgacgg gggacttcga tgtcttccac
      961 tgcggcgacg cgtggcccgg tcccgatgtc gcccggttgc gattcgctgc tgacaaagca
     1021 gagtaattcc acgaccgtcg caagggccaa gcggtcctcc tcgttgtagg cgaggagtcg
     1081 ctgcttgacc acctcatccc tggtagtctc ccaggcgtgt cgccagacaa tggactgcaa
     1141 tcctgaggcg tccgcggcag accagcaaca tcccaggcaa ccggccacgc tcttcaggtc
     1201 attggagtgg accgggaagt agacacggcc gtagatcagc gatagtacgt tgacgctccg
     1261 cgatttgagc ttggcgatca gctcagggtc gccgccgtgg cgggccttca tcttcccaag
     1321 gaagtgcgac tcatagctcc cataatggaa tagcacgaag tcctcgatcg gtcctgcgac
     1381 gtcaaggaac gatctccaca tggacgcctc ttccgccccg tggtccgccc agaagcacag
     1441 ccggcgtcgg gtgctgcctt cctcgacggt taggccgatc agatagtagg agtccgcgtc
     1501 gggaagtccc tccacgtcca gatagagccg cgccttaccg tcgggcagtt ccagcttctg
     1561 ggccacgtag actgtgttat cgcggacggc cagcgcctgg agtgagtggt cgtgcttcgg
     1621 gctcttccac tcgaccgctc gcctcatccg cccgggacgg aaggtgtacg aatactgggt
     1681 gacggtaaaa atcccccgtc ggttcagccc ggcgatgtcc ttcggggaca ggccgcggag
     1741 caggctcagg tcgtcctttt cgacagctgt cgcctgacac acccgccgga actcgcagac
     1801 ccggcagtgg tcgttcagaa tcaatctcgg cggcgactcg gcaccgatca tctccctgac
     1861 ttcttggagg aggcgttcgg tccttcgcac gtcggggttg agccggatct tcgaggctct
     1921 acactcccgc ccgtgccaca ccaccccgct ggcgggcagc ctctcctgga cctgggacag
     1981 gagcagaccg aatagctcca gcagaagcct ctgctccttg tcgacctttc gcccctcatg
     2041 gaacaacatc gggacgtagt ggaagtcccc cagcttcgac ggtccgtccg cccgcttcag
     2101 accgtcgaag cggagcgaca ccaggtcgtc ttccagggtg gcgtccagga cgaacgaagg
     2161 cccggcccgc agggcgctgg cggtcagggg gatgccactc gccacctcgc tttcggggta
     2221 gcgttcgagg atcttgccga tggccgtatt ccgcacctcc cccctggagg cgacgagcag
     2281 cttctcgtaa tccgacgcgc tcccctgctg gcccgccagt ttcaggtggg ccttggtctt
     2341 gcagtgcagg tagccttcca ggacgtcccg ggtaaccttc gtggccatga ttctgttccc
     2401 gaggtctgct cgggtccatc ccgcccggtg agttattccg accggatggc gtgcttctgg
     2461 accgccacat catggcgggg acggacgcac ccgaacccaa ttggtcagct tacacggccg
     2521 ggaacgggaa gtcgtgagcg tatcgccgtt cgcgcccaga ttggcctcct ccagcttcgc
     2581 gtcccagcag ccatttcacg ccgctgtctt gcgccgtgct gaggggtgag acgcgaacca
     2641 gacatccggc agcaactcgt cgagccgccc cgcgggttgg gaaggcaggc gacggaggat
     2701 gtcgtcgagg taggcgaacg ggtcgatgtc gtggtgcttg cagctgccgg tcaaggagta
     2761 caggatggcc gccgtccggc caccccgatc actccccaag aaggtccagt tccgacgccc
     2821 gatcgcctgg gcacgcaaca gcctctcggc gaggttattg tcgatgctca gctccccgtg
     2881 ctcggggtag cgacagagcg ccgcccagtt cgactgcgtg taggcgatcg cctgacctat
     2941 cggactcttg ggcagcacgt ggcggccctg ctcggtcaac cactccccga aggccaccaa
     3001 cagcgggacc gaatccctct ggcgcagggc ataacggtcc tgggccgaag aatcctcggc
     3061 ggcatgctcg atcgcgtaga gctgccggat gcggcccagc gccgcgtgcg ccggcaccag
     3121 cgccgaggtc cgcgcttcga agaacttcct ccggacgtga gcccagcatg cgacctcgat
     3181 cacgtcggga ccggcgcaca tccggtcata gccggagaac gcatcggcct ggaggtaacc
     3241 ccggaacccc ttcaggaact gctccggccc gtcccgcgtc cggcgcggcg tgtagtcgta
     3301 cacgacgtac ggatagcgga ggtcgccgac atagacccag accctgccag tacgggtctt
     3361 cggcagtgtc gggtcccaca ccggcacggg ggtatcatcc gtgtgaatga tcgtcgacca
     3421 caaaacccgc tggaccatca ggtcgtagag cggcgcgagc cggtcggcgg cctgggccat
     3481 ccagccgcag agcgtgctcc gggacagcac cacaccatga cgggcgagga tgtcctcctg
     3541 acggtagagc ggcaggtgat cgctatattt gctggtgatg gtctgcgcca tcagtcccgg
     3601 ccccggcatc cccttgtcga tcggctgggg cggcttgtcc g
//
LOCUS       DBNGZC010000318         1525 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1688833, whole genome shotgun sequence.
ACCESSION   DBNGZC010000318 DBNGZC010000000
VERSION     DBNGZC010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1688833"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..297)
                     /locus_tag="AC16G0_05785"
     CDS             complement(34..297)
                     /locus_tag="AC16G0_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099169.1"
                     /translation="MVSHPDQPVCMKCRHDDRVFPRLDSFDRLRLADDPADPNCGHIY
                     LETVYILECPACGHRQEQVHQRTPYPTLRQAQSELDAHLLGKG"
     gene            complement(322..1290)
                     /locus_tag="AC16G0_05790"
     CDS             complement(322..1290)
                     /locus_tag="AC16G0_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013316.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIR5099170.1"
                     /translation="MVELLAPAGNLSMVEAVVRGGASAIYVGPRGWSRRRDRYELTDD
                     DVREAIGIAHASGVKLRVAVNTNMQSHEIPPMLEKMERFVAWGVDGAIMTDIGAIAEV
                     HRRFPHLTIHASIGANILNDEDVGFYRSIGVSQVVADTKLTLKELASRKERIDVGIEI
                     LIHANKCYTYLGKCWMSPYHRLERTTDPFGKDHFKGSPNRGGLDYRVCLESWDLYSGE
                     TKWEEGVALKNDAFVLLEDIPLLIDLGVHCLKVQGREYPVPLVEGMVRFYRDMIDAYL
                     AHPKGEPFDLTPWGARLAGIEAERNAARSDGTRLLLIEARQPVLAA"
BASE COUNT          259 a          523 c          470 g          273 t
ORIGIN      
        1 taggatttga tggaaaggtc tctgtcgggc gtatcagcct ttgcccagga ggtgggcgtc
       61 caattcgctc tgcgcctggc gcagcgtcgg gtaaggcgtt cgctggtgga cctgctcctg
      121 gcgatgcccg caggcggggc actccaggat gtacaccgtc tccagataga tgtggccgca
      181 gttggggtcc gcgggatcat cggcaaggcg aagccggtcg aaggagtcga ggcggggaaa
      241 cactcgatca tcatgccggc acttcatgca caccggctga tcggggtggc tgaccatggg
      301 cggccgctcc tgggggggtc ctcaggccgc cagcaccggc tgacgagcct cgatcaagag
      361 gaggcgcgtg ccgtcggagc gggccgcgtt tcgctccgcc tcgattccgg ccaggcgggc
      421 cccccagggc gtgaggtcga agggctctcc cttcgggtgc gccaggtagg catcgatcat
      481 atcgcggtag aatcggacca tgccttccac caggggcacg ggatactccc gtccctggac
      541 cttcaggcaa tggaccccca ggtcgatcag gagcgggatg tcctccagca ggacgaacgc
      601 gtcgttcttc agggcgaccc cctcctccca cttggtctcg ccgctgtaca ggtcccagga
      661 ctccaggcag acccggtaat ccaggccgcc ccgatttgga ctgcccttga agtggtcttt
      721 accgaaggga tccgtcgtcc gctccagcct gtggtagggg gacatccagc acttccccag
      781 ataggtgtag cacttgttcg catggatcag gatctcgatc ccaacgtcga tccgctcctt
      841 ccgcgaggcc agttccttca gcgtcagctt cgtgtccgcc accacctggc tcacgccgat
      901 ggagcgataa aagcctacgt cctcgtcgtt caggatgttg gccccgatgc tggcgtggat
      961 ggtgaggtgg gggaagcgcc ggtgcacctc cgcgatagca ccgatgtccg tcatgatcgc
     1021 cccgtccacc ccccaggcca caaagcgctc catcttctcc agcatgggtg ggatctcgtg
     1081 cgactgcatg ttggtgttga cggccactcg cagcttgacc ccgctcgcgt gggcgatccc
     1141 gatcgcttca cgcacatcat cgtccgtgag ctcgtaccgg tctcgccgcc ggctccagcc
     1201 gcgcggcccc acatagatgg cgctcgcgcc gcctctcacc accgcctcga ccatggaaag
     1261 attgcctgca ggcgccagga gctcgaccat gccgcacctc atcatgaagc cacggcccgc
     1321 ccccgaacgg gccatccatc atgccccatg gggccaccga gggagtagcc cccaccgctc
     1381 attgcctgct acgactccga cggcgagatc ggcaggaacg acgcggccgc cagcggccgg
     1441 aaacctgcgt agtccattcc gcttttgccg aagtagaacc cattgcacaa cccgatatgc
     1501 gcgtggccgc tcaggacacc ccacc
//
LOCUS       DBNGZC010000319         3403 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_320787, whole genome shotgun sequence.
ACCESSION   DBNGZC010000319 DBNGZC010000000
VERSION     DBNGZC010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_320787"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..3007)
                     /locus_tag="AC16G0_05795"
     CDS             complement(101..3007)
                     /locus_tag="AC16G0_05795"
                     /inference="COORDINATES: protein motif:HMM:NF014763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5099171.1"
                     /translation="MKIRIRLFWKYVVLFVILVSGALVTSGLVEIYFSYRENKTVLVS
                     LQRETAAAAASRIEQFIQQVEDQIGWTSQAMFLPKGAALDQRRFDYLRLLKQAPSVTE
                     VSLLNSSGKEQLRVSRLAMDVLGSQADFSHEPKFLEAKSGKTYLGPVYFRKESEPYMT
                     IAMAGAGQDGGVTIAEVNLKFIWDVVSRIKVGEAGYAYAVDSRGRLIAHPDISLVLQK
                     KDLSSLPQVQAARSAPLVAGPGQHAAGVARDLNGREMLTAYAAIAPLGWFVFVEQPLK
                     EAFAPLYSAILRTTILLVIGLGMSILVSVFLAQKMVTPIRALQSGAARIGAGDLGHRL
                     KVGTGDELEALADQFNSMTAQLQESYANLEQKVEARTRELSEALEQQTATSEVLKVIS
                     RSTFDLQPVLQTLVENAVRLCNAESGHIHRFDGEVLHHSVAYGGSPELTDFIRHHPIH
                     PGRGTVAGRVLLQKRTVHIPDVLADPEYEFSNAQKVGGFRTVLGVPLLRGEDPLGVFV
                     LWRNEVKPFTDKQIELVAGFADQAVIAVENVRLFNEIQERTRALESSLEEVHALSEVS
                     RAVSSSLELRQVLHEIAEQAAKLCAADAGFIQEYVAAAEEFRISISWGASDEFLRAIQ
                     TAQVTLGKGATGRSAAMGRPVQIPDILAEPDYAFRDILTREGYRAVLSVPMQRDGHIV
                     GTVVVTRRTPGAFSERHVNLLTTFASQTTIAIEHARLFKEIQDKGHQLEIVSQHKSQF
                     LANMSHELRTPLNAILGYTELILDSIYGEIPEKVQDVLGRLQKSGRHLLSLINDVLDL
                     SKIEAGQLTLALSDYSMKEVIHTAFTTVESLAAEKRLSLTARVPPDLPKGRGDDRRIT
                     QVFLNLLCNAIKFTEVGEVSVQVTTTADAFLVAVTDTGPGIAPADQQKIFQEFHQVDS
                     SSTRKKGGTGLGLAIAQRIIALHGGRIWVESSPGKGATFWFTLPIHVKQPTEAP"
     gene            complement(3248..>3403)
                     /locus_tag="AC16G0_05800"
     CDS             complement(3248..>3403)
                     /locus_tag="AC16G0_05800"
                     /inference="COORDINATES: protein motif:HMM:NF016292.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HIR5099172.1"
                     /translation="YVDRVLKGEKPADLPVQTPTKYELVINLKTAKALGLTIPQSILV
                     RADKVIQ"
BASE COUNT          594 a         1065 c         1055 g          689 t
ORIGIN      
        1 cccgtcgttg cctccagcag ggcgaagccg gcgctggtga ggaggtcccg caggatcttc
       61 cggttgtcct cctggtcctc cacgatcagg atgcgtctgg tcatggcgcc tccgtcggct
      121 gcttcacgtg gatggggagc gtgaaccaga aggtggcgcc tttccccgga ctcgactcca
      181 cccagatgcg ccccccgtgc agggcgatga tccgctgggc gatggccagg ccgaggccgg
      241 tgcccccctt cttccgggtg ctggagctgt ccacctggtg gaactcctgg aagatcttct
      301 gctggtcggc gggggcgatc ccgggccccg tgtcggtgac cgcgacgagg aacgcgtccg
      361 ctgtcgtcgt cacctggacg ctcacctcgc ccacctcggt gaacttgatg gcattgcaga
      421 ggaggttcag gaagacctgg gtgatccgcc ggtcatcacc ccggcctttc ggcaggtcgg
      481 ggggcaccct ggccgtcagg ctcagcctct tctccgccgc cagggattcc accgtggtga
      541 aggctgtgtg gatgacctcc ttcatcgagt agtcgctcag ggcgagggtg agttggcccg
      601 cctcgatctt ggagagatcc aggacatcgt tgatcaagga gagcaggtgg cgcccgctct
      661 tctgcagtcg tcccagcacg tcttggacct tttcgggaat ttccccgtag atactgtcca
      721 ggatcagctc cgtgtacccc aggatggcgt tcaggggggt gcgcagctca tgactcatat
      781 tggcgagaaa ctgagacttg tgctggctca cgatctcaag ctggtggccc ttgtcctgga
      841 tctccttgaa cagccgagcg tgttcgatgg cgatggtggt ctggctggcg aatgtggtca
      901 ggaggttcac atgccgctcg ctgaaagcac cgggcgtcct gcgtgtgacc accaccgtcc
      961 ccacgatgtg tccgtcccgc tgcattggaa cggagaggac agcgcggtaa ccctccctgg
     1021 tcaggatatc ccggaaggca tagtcgggct cagcgaggat atccgggatc tgcaccggcc
     1081 tgcccatggc cgcgctgcgg cccgtggccc ctttgccgag cgtcacctga gcggtctgaa
     1141 tggcgcggag gaactcgtca ctcgcacccc aactgatgct gatgcgaaac tcctcagccg
     1201 ccgcgacata ctcctggatg aacccggcat ctgccgcgca gagcttggcg gcctgctcgg
     1261 cgatctcgtg gaggacctgt ctcagttcga gggaggagct gaccgcccgg ctcacctcgc
     1321 tcagggcgtg gacctcctcc aacgacgact ccagggcccg ggtccgctcc tgtatctcgt
     1381 tgaacaggcg gacattttca acagcaatga cggcctggtc ggcgaagccc gcgacgagtt
     1441 cgatctgctt gtcggtgaag ggcttgactt cgtttctcca aagcacgaaa actccgagag
     1501 gatcttctcc cctgagcagg ggcaccccca gcaccgttcg gaaccccccc accttctgag
     1561 cgttggagaa ctcatattcg gggtccgcca aaacgtctgg gatgtgtacc gtgcgctttt
     1621 gaagcagcac tcgccctgcc acggtccctc gcccaggatg gattggatgg tgccggatga
     1681 aatctgtcaa ttcgggagac ccgccataag caaccgaatg gtggagtacc tcgccgtcga
     1741 acctgtggat atgaccggat tcggcgttgc acagtcgcac cgcgttctcc accagggttt
     1801 gcagaacagg ctgcagatcg aaggttgagc ggctgatgac cttcaggact tcgcttgtgg
     1861 ccgtctgctg ctccagcgcc tcgctcaact cccgagtccg tgcctccacc ttctgctcca
     1921 ggtttgcgta cgactcctga agctgggcgg tcatactgtt gaactgatcg gccagggcct
     1981 ccagttcgtc tcccgtccct accttcaggc ggtgacccag atcgcccgcg ccgatccgag
     2041 ccgctcccga ctggagcgct ctgatgggag tgaccatctt ttgggccagg aacacgctga
     2101 ccagaatcga catcccgagt cctatgacca gcaggatagt cgttcgaagg atggcagagt
     2161 agaggggggc aaaagcttcc ttcagaggct gctccacgaa gacgaaccat cccagcggag
     2221 cgatggccgc ataggcagtg agcatctctc ggccattcag gtcccgggcg actccggccg
     2281 cgtgctggcc cgggcctgcc accagagggg ctgaacgggc ggcctgcacc tgtggcagtg
     2341 aggacaggtc cttcttctgg aggacgaggc tgatatccgg gtgggcgatg aggcgaccac
     2401 gggaatccac cgcgtaggca tacccggcct ccccgacttt gatccgggag accacgtccc
     2461 agatgaattt cagattgacc tcggcaatcg tcacgccccc atcctgcccc gcgccggcca
     2521 tggcgatggt catgtacggc tcggactctt tgcgaaagta gacggggccg aggtacgtct
     2581 tcccggattt ggcctcaagg aatttcggct cgtgggagaa gtccgcctgg cttcccaaca
     2641 cgtccatggc caggcgggat acccggagct gctctttccc ggaggagttc agaaggctga
     2701 cctcggtaac gcttggggcc tgcttcagga gccggaggta gtcgaatctt cgctggtcga
     2761 gggcggcacc cttcggcagg aacatggcct gcgacgtcca tccaatctgg tcttcgacct
     2821 gttggatgaa ttgttcgatt cgagaagcgg cggccgccgc cgtctcccgc tggaggctca
     2881 ccagcaccgt tttgttctcc cgataggaga aatagatctc cacgaggcca ctcgtcacca
     2941 gcgccccgct gaccaggatc acgaagagga cgacgtattt ccagaagagg cggatgcgaa
     3001 tcttcatggc gcctcgatgc tgatctgggg ccatctcccc aagggttccc gtggccgtgc
     3061 cgtgcccctt cgcttcctgg agggagggtg gcacatggtt gccatgccgt caatgacctc
     3121 gcctgatgca ccacccccga tcaccggagc acttcgtccg ttcggatgag gcggccggga
     3181 gcgagcgcca cgctgaatat cgcatttgac ctacttcgaa atccgcaatt ccaaattcga
     3241 aattgtctca ctggatcacc ttgtccgccc ggaccaggat ggactgcggg atcgtgaggc
     3301 cgagcgcttt ggcggtcttg aggttgatga ccagttcgta cttggtcggt gtttgcactg
     3361 gcagatcggc tggcttctct cctttgagaa cgcgatcgac ata
//
LOCUS       DBNGZC010000320         2642 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1982369, whole genome shotgun sequence.
ACCESSION   DBNGZC010000320 DBNGZC010000000
VERSION     DBNGZC010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1982369"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="AC16G0_05805"
     CDS             <1..348
                     /locus_tag="AC16G0_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007004805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099173.1"
                     /translation="IQQQEFILAARAMGANDRHLIFRQLLPNALAPVLVAATLNVATA
                     ILLESSLSYLGYGIQPPTASWGNMLNNAQTYVFYAPWVAVYPGIMITLTVLSFNFAGD
                     GLRDALDPRLSLS"
     gene            404..2113
                     /locus_tag="AC16G0_05810"
     CDS             404..2113
                     /locus_tag="AC16G0_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5099174.1"
                     /translation="MKKQERQDKGMASGLLSRRQLLKGAAVAGVAATGLLGVGRGETA
                     QPARPAPAPTLQPQKGGQIIVGISQEPQNFNPIMPTIEAQRGVHMAIYDDLWRIGPKG
                     NYIPNLATEIPSQTNGGISKDGTLFTIKLRRDTKWHDGQPFSAKDVVFTWQTIMNPKV
                     SAFSTVGFDQIDSMWAVDEATLKFKLKQSYAPIFAILSDMFVVPEHILSKSPDINKDE
                     FNTKRPIGTGAFKFVEWAAGDHITLEANKAYHGPGPYIDRLIFKYISDLTVLFTQFKT
                     GEIDITGLQGIPVDLYPEAKTLTNITLHVNPGTTYDCIAPNFLLPLFQDKRVRQALYY
                     GMDKKPMVEKIFLGLVAEAETYIPPQSWAYNPKIKGHHKYDPEKAKKLLDAAGWKVGP
                     DGIRVKDGKRLSFENACTAGNKGREQMQQLLQQQWRQIGVEMKINNKPAAVLFGDFYR
                     LSKFETIINGIGMGADPEHSFRLHSKYIPAKGGMGRNSIAYENKEVDQLLEAGAHEMD
                     QEKRKKIYYKLQEVLADDLPYLPIHHYVIIRATKASIQGFQPNSNMQDYTWNPNEWWI
                     KKA"
BASE COUNT          559 a          840 c          789 g          454 t
ORIGIN      
        1 atccagcagc aggagttcat cctggcggcg cgcgccatgg gggcgaatga tcggcacctc
       61 atcttccggc agctcctccc caacgccctg gccccggtgc tggtggccgc caccctgaac
      121 gtggccaccg ccatcctgct ggagtcttct ttgagctacc tcggctatgg tatccagccc
      181 ccaacggcga gctggggtaa tatgctgaac aacgcacaga cctatgtgtt ctacgccccc
      241 tgggtggccg tctatccggg gatcatgatc accctgaccg tcctgagctt caattttgcc
      301 ggcgatgggc tgcgcgatgc gctggatccg cgcctgtcac tgtcgtgagg cgcgacgtcg
      361 gacgcgccgg gaaggaaacg tggctggaga caaggaggag gccatgaaga aacaggagag
      421 gcaggacaag ggtatggcga gtgggctgct ctcgcggcgg caactgttga agggggcggc
      481 cgtggcgggc gtggcggcga ccggcctgct cggcgtgggc cgcggagaaa cggcccagcc
      541 cgccagaccg gccccggccc ccacgctcca gccgcagaaa ggggggcaga tcatcgtcgg
      601 gatctcccag gagcctcaga acttcaatcc catcatgccc acgatcgagg cccagcgggg
      661 cgtccacatg gccatctatg acgacctgtg gcgcattgga cccaagggca attacatccc
      721 gaacctggcg acggagattc cgagtcagac caacgggggc atctccaagg atggcaccct
      781 cttcacgatc aagctgcgca gggataccaa gtggcacgat ggccagccgt tcagcgccaa
      841 agacgtcgtg ttcacctggc aaaccatcat gaaccccaag gtgagcgcct tttccacggt
      901 gggcttcgac cagatcgact ccatgtgggc ggtggacgag gcgacgctga agttcaagct
      961 gaagcagtcc tatgccccca tctttgccat cctgtcggac atgttcgtgg tgcccgagca
     1021 catcctgagc aagagtccgg atatcaacaa ggacgagttc aacaccaagc gacccatcgg
     1081 gaccggcgcg ttcaagttcg tcgagtgggc ggccggtgac cacatcaccc tggaggccaa
     1141 caaggcgtat cacgggccag gaccctacat cgaccgcctg atcttcaagt acatctccga
     1201 tcttaccgtt ctcttcaccc agttcaagac gggcgagatc gacatcacgg gactccaggg
     1261 catccccgtg gacctgtatc ccgaggccaa gacgctgacg aatatcacgc tccacgtgaa
     1321 tcccggaacc acctacgact gcatcgcgcc caacttcctg ctcccgctgt tccaggacaa
     1381 gcgggtccgg caggcccttt actacggcat ggacaagaag cccatggtgg aaaagatctt
     1441 cctggggttg gtggcggagg ccgagactta cattccaccc cagtcctggg cctacaatcc
     1501 caagatcaag gggcaccaca agtacgaccc cgagaaggcg aagaagctcc tggatgcggc
     1561 aggctggaag gtggggccgg acgggatccg ggtcaaggac ggcaagcgcc tctccttcga
     1621 gaacgcctgc accgccggca acaagggacg ggaacagatg cagcagctcc tccagcagca
     1681 gtggcggcag atcggcgtgg agatgaagat caacaacaag ccggcggcgg tcctcttcgg
     1741 cgacttctac cgcctgtcaa agttcgagac catcatcaac ggcatcggca tgggggccga
     1801 cccggagcac agcttccgcc tgcacagcaa gtacatcccc gccaaagggg gcatgggccg
     1861 aaactccatc gcctacgaaa acaaggaggt ggaccagttg ctggaggccg gcgcccacga
     1921 gatggatcag gagaagcgca agaagatcta ctacaagctg caggaggtgc tggcggacga
     1981 tcttccctac ctgcccatcc accactacgt gatcatccgg gcgacgaagg ccagtatcca
     2041 gggatttcag cccaactcca acatgcagga ctacacctgg aatcccaacg agtggtggat
     2101 caagaaggct tgagagggag gtgcgatcca ctcctgggcg gtcgatgcgg tgtgagaagc
     2161 agaaacggcg gctccctgga tgggggggcc gccgtttgga tgggccggta tccggcttga
     2221 cgatcagctc tggggcaagg gttgcgttgg caccggcgta gaagcctcac tgtgacttcc
     2281 accacagttg gccagtgcgg ttcccgcgac ccccagcagc aaaccaagag cgagtaccag
     2341 gctcagtagc tttcgcatag cttcctcctt ctcccttttt tccttaggcc gctccatccc
     2401 ctccccgatc ccattgggca cttatacggg acttatccgg tcctgtcaag aaatatccag
     2461 gcatcactta tgttgtaaac aagcatggct gacacttcca tgctccaggt gtggatgctc
     2521 gcgcctctga cttctctcca accctccttc aagtgcctcc ccggcccggg gaccctcgcc
     2581 tggcaggatt atgcctgggc gccggacctg gtgctgatcc cccgcgacac gcaggtgacc
     2641 ac
//
LOCUS       DBNGZC010000321         1674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1493293, whole genome shotgun sequence.
ACCESSION   DBNGZC010000321 DBNGZC010000000
VERSION     DBNGZC010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1493293"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="AC16G0_05815"
     CDS             complement(<1..858)
                     /locus_tag="AC16G0_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5099175.1"
                     /translation="MRRGAVAVAAAGVLGVALLPFLVSDYLVGFVFNMFLFLILAYGW
                     NLLSGYSGYLSFGQISFFGLGAYTMAILVFRLHWPWPLGVLCGTWAAVVLAVPLGWLM
                     LRLRGPYFALGMLGFVQVLFMVASAASSLTGGGEGIYLPPEASLMSLYYSAAAVVALA
                     IATTWTVERSAFGLRLQGIREDELAAEAMGVNTTRNKLAVFVLSSVFPAVAGGLYGWR
                     LSYIDPSSAFPGQYEMQSILMTIFGGAGTIWGPLLGGAALSIMAEVIWARFAELHLLL
                     FGSLIVLVLL"
     gene            complement(855..>1674)
                     /locus_tag="AC16G0_05820"
     CDS             complement(855..>1674)
                     /locus_tag="AC16G0_05820"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5099176.1"
                     /translation="VYGLMSLGINLVWGVMGVINLAHGDLIMAAGFLAFWIFSHFSLN
                     PLLTVPIGMAMACAVGVLSQRFLLGQLPQDRGAVKSSLLMTFGISYIVINVAQLLWGN
                     EFQTIAFLAGSWKVGGIDFAKGRTIAFLAAACITLILWAFLQFTNAGRAVRAVSQNPD
                     GASVCGIDIGGIRVFSFGLGASLAGAAGVLLVMIFAISPAMGSGYTVRSFAVTVIGGL
                     GSFLGALFGAMILGLAEVFTGYLVSAQVANGVAFILFLIVLLVLPQGLAGLGRR"
BASE COUNT          391 a          550 c          538 g          195 t
ORIGIN      
        1 caagagcacc agaacgatca aggacccgaa cagcagcaga tgcagctccg caaatcgagc
       61 ccagatcacc tcggccatga tggagagagc ggccccaccc aggagcggtc cccagatcgt
      121 tccggcaccg ccgaagatgg tcatcaagat cgactgcatc tcgtactggc ccggaaaggc
      181 cgacgacggg tcgatgtagc tgagacgcca gccgtagagc ccgcccgcca ccgcaggaaa
      241 cacagacgag aggacgaaga cggccagctt gttgcgggtg gtgttgaccc ccatggcttc
      301 ggctgccagc tcgtcttccc ggatcccctg taaccggagg ccaaacgccg agcgctcgac
      361 cgtccaggtg gtggcgatcg ccagagcgac cacggccgcc gccgaataat agagcgacat
      421 caagctggcc tcggggggga ggtagatccc ctcgccgcca cccgtgaggg aggacgcggc
      481 cgaggcgacc atgaagagca cctggacgaa tcccaacatg cccagggcga agtacggccc
      541 gcgcaggcgg agcatcagcc accccagggg aacggccagg accactgccg cccaggtgcc
      601 gcacagcacg cccagaggcc agggccagtg cagccggaac accaggatgg ccatggtgta
      661 ggcacccagg ccgaagaatg agatctgtcc gaaggagagg tagccggaat acccggagag
      721 caggttccat ccgtacgcca ggatgaggaa caggaacatg ttgaagacaa accccacgag
      781 gtaatcggag accaggaagg gcaagagggc caccccgagc accccggcgg cagcgacggc
      841 gacggctccc cgcctcatcg tcgtcccaga cccgccagtc cctggggaag gaccagcagg
      901 acaatcagga acaggatgaa ggccacgccg ttggcgacct gggcgctgac caggtagccc
      961 gtgaacactt ctgccaggcc gaggatcatc gcgccgaaca aggcgcccag gaaggagccg
     1021 aggccgccga tcaccgtgac ggcaaaggag cggacagtgt atccggagcc catggccggc
     1081 gagatggcga agatcatcac cagcaacaca cccgccgccc cggccagtga ggcgccgagt
     1141 ccgaagctga agacacggat ccctccgatg tcgatcccgc agacggatgc gccgtcgggg
     1201 ttctgcgaca ccgccctcac ggcccggccg gcgttggtga actggaggaa cgcccagagg
     1261 atcagcgtga tgcacgccgc ggccaggaac gcgatcgtcc ggcccttggc gaagtcgatc
     1321 ccacccacct tccaggagcc ggcgagaaag gcaatcgtct ggaattcatt tccccacaga
     1381 agctgcgcga cgttgatcac gatgtaggaa atgccaaagg tcatcaggag ggacgacttg
     1441 acggctccac ggtcctgggg aagctgtccc agcaggaatc gctgggacag caccccgacc
     1501 gcacacgcca tggccatccc gatgggcacg gtcagcagcg ggttcaggct gaagtgggag
     1561 aagatccaga aggcgaggaa acccgccgcc atgatcaggt ccccgtgggc caggttgatg
     1621 acccccatca cgccccacac caggtttatc cccagggaca tcaggccgta cacg
//
LOCUS       DBNGZC010000322         2250 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_417106, whole genome shotgun sequence.
ACCESSION   DBNGZC010000322 DBNGZC010000000
VERSION     DBNGZC010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_417106"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="AC16G0_05825"
     CDS             <1..706
                     /locus_tag="AC16G0_05825"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5099177.1"
                     /translation="ELAHMSRVMLMGELTASLAHELKQPLTAIVTNAYSGHRFLAEPV
                     PQLDGVREILADIVADGQRAGAVIDRVRGFLKKDGSQFRPLDLNELIREIQGLTRTDA
                     IIRHQPITLALAPRLPAVVGDRIQLQQVLLNLILNGMDAMAGQAPAKRLLTIHTAPET
                     TSVRVGVEDRGAGVPLDQLGQIFTAFVTTKPNGMGMGLAISRSIVEAHGGRIWAENNP
                     EGGATFWFTLPVQSQG"
     gene            1101..1415
                     /locus_tag="AC16G0_05830"
     CDS             1101..1415
                     /locus_tag="AC16G0_05830"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5099178.1"
                     /translation="MKTILASALLFLTLTSSALAQGSAGSPESGKALAGRWCAACHLV
                     QLRLTTIDPPTFTAIANDSSKTPGYLRTFLISPHKDMPPIQLTPSQIEDLIAYLSTLK
                     RP"
     gene            1715..>2250
                     /locus_tag="AC16G0_05835"
     CDS             1715..>2250
                     /locus_tag="AC16G0_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099179.1"
                     /translation="MISRSFRSRLTGLLVCLLACGCAGTGTQEVEVYEPLPTQPFAKF
                     LIVGIHENGRTRRLFENAFVAELAGHGVEGVQSYRFIYEERAINVERVVRAVLESGAD
                     AVITVRAVDVDLQPKTARPIQRDRFEFDLASDNPERRLLPKRDKVTLQTNVYQAANKH
                     LVLSAMSRVVSPDSVEAIG"
BASE COUNT          403 a          763 c          654 g          430 t
ORIGIN      
        1 cgagttggcc cacatgagtc gggtaatgct catgggcgag ctgactgcct cgctcgccca
       61 tgagttgaag cagccgctta ctgccatcgt gaccaacgcc tactccgggc accggttcct
      121 ggcggagccg gtcccccagc tcgatggggt gcgcgagatc cttgccgaca tagtggcaga
      181 cgggcaacgg gctggggcgg taattgaccg ggtgcgcggc ttcctcaaga aggatgggag
      241 ccagttccgc ccgctggatc tcaacgagct catccgagaa atccagggcc ttacgcggac
      301 tgacgccatc atccgtcatc agcccatcac ccttgcactc gcccctaggc tgcctgcggt
      361 ggtgggggac cggatccagc ttcagcaggt gctcttgaac ctcatactga acggcatgga
      421 cgccatggcc gggcaggcgc ccgctaagcg tctgctcacc atccacactg cgcccgagac
      481 gacgtccgtc cgcgtgggcg tcgaggaccg gggcgcgggc gtcccgctgg accaactggg
      541 ccagatattc accgctttcg tcacgaccaa gcccaacggc atgggcatgg gcctcgccat
      601 cagccgctct atcgtggagg cgcacggcgg gcggatctgg gctgagaaca accccgaggg
      661 cggcgccaca ttctggttca cattgcccgt gcagtcgcag ggttgacgct gccacgcgcc
      721 cgggcgtaga ctccgcccgc tcgcgcccca catcctctgc tggcagacgc atgggcgagc
      781 ggaccgcgta tctattatgg gcccgggtcc ccgacaccct catagagcga accggtcgtc
      841 cgctccaaga aggagctcag acctggccgc aagcgtcatt gtcctatccg tgctcccctc
      901 tgctcctgtg ttgacatcag cccatatggg accaaggtcc aattgacatc tctggggggg
      961 ggtccagtat gttctcacca ggctcggaag actggagagg gttgcggatg gatctttttg
     1021 ggggacgcca tcccactagg cgctgtcagt ttgttcttct gtccgccgtc cctgacgaac
     1081 tacagaccag acgtcgagtc atgaaaacca tcctcgcgag cgcccttctt ttccttacgc
     1141 taactagctc agcgctcgcc caaggatcgg cgggttcccc cgaatccggg aaagcacttg
     1201 ccggacggtg gtgcgcagcc tgccatttgg tccagctccg gcttaccacc atcgatccac
     1261 ccaccttcac ggctattgcc aacgattcca gcaagacccc aggctacctt cggacgttcc
     1321 tcatcagtcc acataaggac atgccaccca ttcaactcac gccatcgcaa atcgaggatc
     1381 tcatcgccta ccttagtacc ctgaagcgac cctagccgca gtaatatctc ccagccccat
     1441 ctgggggttc agacccttcc tatttctctt cctctacgtg aatctgctcg acaaccgcag
     1501 atggttctcg gcgtcctccc tttccaacgc attgctcagc cggagccacc gtgcgatatg
     1561 cggaggggct tctacaccgt taccaacccc gcgccaacag ccgacttacc aacgtccttg
     1621 acgccttctt cattctcgta cagaatccgt ggcgtccttg ccgggccaga accacacccc
     1681 ctggcccctg tctgcgaggg cacggagggg tgcgatgatc agtcgttctt tccggtctcg
     1741 tttgacagga ctcctggttt gcctcctcgc ctgcggctgc gcgggcacgg gcacgcagga
     1801 ggtcgaggtg tacgagccgc tcccgaccca gcccttcgcg aagttcctga tcgtggggat
     1861 ccacgagaat ggccggaccc gccggctctt cgagaacgcc ttcgtcgcag agctggccgg
     1921 gcacggggtg gagggggtcc agagctatcg cttcatttac gaggagcggg cgatcaatgt
     1981 cgagcgggtc gtgcgtgccg tcctggagtc cggggccgat gccgtgatca ccgtccgcgc
     2041 ggtcgatgtg gatctgcagc cgaagaccgc gcggcccatc cagcgagaca ggtttgagtt
     2101 cgacctggcc tccgacaacc cggagcgaag actcctcccg aaacgcgaca aggtcaccct
     2161 gcagaccaac gtctaccagg cggccaacaa gcacttggtg ctctcggcca tgtcccgcgt
     2221 ggtcagtccc gactcggtcg aggccattgg
//
LOCUS       DBNGZC010000323         2556 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2472085, whole genome shotgun sequence.
ACCESSION   DBNGZC010000323 DBNGZC010000000
VERSION     DBNGZC010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2472085"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="AC16G0_05840"
     CDS             <1..699
                     /locus_tag="AC16G0_05840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5099180.1"
                     /translation="GVGLLALAAALMILAALRRLDRPAAGESGPREVDGAKNGWGIRD
                     ARGFQYLSAIHSIDNGTRSAFLTYLPFLLVNKGAGMDMVGLALGLTFTGGAVGKFVCG
                     AVAERLGIIRSVVLTEAATALGICGLLVLPLRPSFFLLPILGIALNGTSSVLYGTVAE
                     LVVPERRSRAYGLFYTVGIGSGAAMPPAFGLLSDWVGLSTTLGVMALLVFTTIPLAQL
                     LRPALLETAPPSPR"
     gene            1061..1552
                     /locus_tag="AC16G0_05845"
     CDS             1061..1552
                     /locus_tag="AC16G0_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5099181.1"
                     /translation="MPKTLVIHPDKCTACRACELACSMKHYGEFNPSRSRIQVNIFLE
                     EALYIPTTCTQCDQAWCAKICPTNAIVRDQATMAYKVLDDKCVGCKMCVLACPFGSVV
                     LFPEKGKAEKCDHCLSIDGDPECVKFCTPKALEYMDEDNEVRNRQMATALKLKETFKE
                     VTV"
     gene            1552..>2556
                     /locus_tag="AC16G0_05850"
     CDS             1552..>2556
                     /locus_tag="AC16G0_05850"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR5099182.1"
                     /translation="MGGWRGEVLRVNLTKGTVKKEKLDPKLARAYIGGRGLGTKMLYD
                     ELDPRVDPLSANNKLIMATGPLTGTNASTGGRYMVLTKSPLTGAIACSNSGGYMGAEI
                     KFCGHDFLIFEGRAKTPVYLWLNNDKAELRPADKVWGKTTHETEDRLRAETHEEAKVC
                     SIGPAGEKQALTACVMNDKHRAAGRSGVGAVMGSKNLKAVVAKGFQSVQVARPKEFMA
                     ACLAVVKKLKESAVTGGGLPKYGTPILVNVINAHGFLPTRNFQQGQFEGADKLSGETI
                     ADTILLKNKGCFACTIACARVSAVKEGRYTGAGEGPEYESGWALGISTGVSDLSAVTK
                     A"
BASE COUNT          463 a          829 c          840 g          424 t
ORIGIN      
        1 ggcgtggggc tgcttgcgct cgccgccgcg ctgatgatcc tggccgccct ccgaagactc
       61 gaccgtcctg cggcgggcga gtccgggccg cgggaggtgg atggcgcgaa gaacggctgg
      121 ggaatccgag atgcgcgcgg attccagtac ctctcggcca tccacagcat cgacaacggg
      181 acgcgcagcg ctttcctcac ctatcttccc tttctcctgg tgaacaaggg tgccggcatg
      241 gacatggtgg gtctcgcgct cgggctcacg ttcaccgggg gcgcggtggg gaaattcgtc
      301 tgcggcgccg tggccgagcg cctggggatc atccgcagcg tggtcctgac cgaggcggcc
      361 acggctctcg ggatctgtgg gctgctcgtc cttcccctgc ggccctcctt ttttctcctg
      421 cccatcctcg gcatcgcgct gaatggaacc tcgtcggttc tgtacgggac ggttgccgaa
      481 ctggtggtgc cggagcggcg ctccagggcc tacggcctct tctacaccgt gggcatcggt
      541 tccggcgcgg ccatgcctcc cgccttcggc ctgctctccg actgggtggg tttgtccacc
      601 accttgggcg tgatggcgct cctggtcttc accacgatcc ctttggccca gctcctccgc
      661 ccggcacttc ttgagacagc gccgccatcg ccgcgatgag gtaaacgggc ttggcctccc
      721 ggtcgttctc cgcattccga ggcgccgaaa tcagctgccc cgatcgccga attcgatcgc
      781 cgaattctcc cgtgacatac aggagtcagc aggtttagat acggtccatg ccgcctgtcc
      841 aggcgggaat tcacgtacaa tcctccgtct ctccggccgc ggggagacga acttttctcc
      901 acccagtccg cgaaagggtc accagcatga acgccggccg accctccggt gcctgcaggc
      961 ttcttaccat cagagaccta gacgatccac cgaggaacgc attgctcgct cgcggacggt
     1021 gatgtctccg cgtgtcgggc cctgaggagg ggagggacca atgccgaaga cgctggtgat
     1081 tcacccggac aaatgcaccg cctgtcgggc atgcgagctg gcctgctcca tgaagcacta
     1141 tggagagttc aaccccagcc gctcccgcat ccaggtcaac atctttctgg aggaggcact
     1201 gtacatcccg acgacctgca cccaatgcga ccaagcctgg tgcgcgaaga tctgtccgac
     1261 caacgccatc gtccgagacc aagcgaccat ggcctacaag gtcttggacg acaaatgcgt
     1321 gggctgcaag atgtgcgtcc tggcctgccc cttcggcagc gtcgtcctct tcccggagaa
     1381 ggggaaggcg gagaagtgcg accactgcct ctcgatcgat ggtgatccgg aatgcgtgaa
     1441 gttctgcacg cccaaggcgc tggagtacat ggacgaggac aacgaggtgc gcaatcgaca
     1501 gatggccacc gccctgaaac tcaaggagac ttttaaggag gtgacggtct gatgggaggc
     1561 tggagggggg aggttctccg cgtgaatctc acgaagggga ccgtcaagaa ggagaagttg
     1621 gaccccaagc tggcgcgggc ctacatcggc gggcgcggtc tggggaccaa gatgctgtac
     1681 gacgaactcg accccagggt ggatccgctc agcgcaaaca acaagctgat catggcgacc
     1741 ggcccgctca ccgggacgaa cgcctccacc ggcggccgct acatggtgct cacgaagtcg
     1801 cccctcacgg gcgccatcgc ttgcagcaac tcgggcggct acatgggggc cgagatcaag
     1861 ttctgcggcc acgatttcct catcttcgag ggccgggcca agacgccggt ctatctctgg
     1921 ctcaacaacg ataaggcgga gctccgcccg gccgacaagg tatggggaaa gaccacccac
     1981 gagaccgagg accgcctcag ggccgagacc cacgaggagg ccaaggtctg ctccatcggg
     2041 ccggcgggcg aaaagcaggc gctgaccgcg tgcgtgatga acgacaagca ccgagccgcg
     2101 ggccgctccg gcgtgggcgc ggtcatgggg tccaagaacc tcaaggcggt ggtggccaag
     2161 ggattccagt ccgtccaggt ggcccggccg aaggagttca tggcggcctg cctggcggtc
     2221 gtcaagaagc tgaaggagtc cgcggtcacg ggcgggggcc tgcccaagta cgggaccccc
     2281 atcctggtga acgtgatcaa cgcccacgga ttcctgccca cccggaactt ccagcagggc
     2341 cagttcgaag gggccgacaa gctgagcggc gagaccatcg ccgacacgat cctgctcaag
     2401 aacaaggggt gcttcgcctg caccatcgcc tgcgcccggg tgagcgcggt gaaggaggga
     2461 cggtacacgg gggcgggcga ggggccggag tacgagagcg gctgggccct ggggatctcc
     2521 accggggtga gcgacctctc cgccgtcacc aaggcc
//
LOCUS       DBNGZC010000324         5085 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_124164, whole genome shotgun sequence.
ACCESSION   DBNGZC010000324 DBNGZC010000000
VERSION     DBNGZC010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5085)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5085)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5085
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_124164"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..1195
                     /locus_tag="AC16G0_05855"
     CDS             275..1195
                     /locus_tag="AC16G0_05855"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR5099183.1"
                     /translation="MCWRCDSHDKNEDDRAIDAALEEAVRTHLSRSPVSRRTLLRAGL
                     GLLAMPAAQVLQSCATVPVTGRSQLRLVSESEEAQLGVTAFQQLRQEEVQKGRLLSER
                     EDPTSHAWVKRVSDRIIRASGLANVYNWDYMLINAPKTVNAAAISGGRIIVYTGILPV
                     AESDAGLATILGHEVGHVMAHHTAERVSQSQLVNVGAALTGEIAGPLLGAVVPAAAEL
                     GYLLPYSRAQESEADHIGLLLMAKAGYDPREAVALWQRMDRSTGGSGRTPEFLSTHPN
                     PGTRIAALEGWLPQAWPYYQNNSLPLPNLQ"
     gene            complement(1348..1512)
                     /locus_tag="AC16G0_05860"
     CDS             complement(1348..1512)
                     /locus_tag="AC16G0_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR5099184.1"
                     /translation="MPTYEFLCTKCQKEFTVTMTIKERGEKPPACPSCGSSELETLIG
                     SFFAKTSRKS"
     gene            complement(1627..2289)
                     /locus_tag="AC16G0_05865"
     CDS             complement(1627..2289)
                     /locus_tag="AC16G0_05865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5099185.1"
                     /translation="MATNLGLRRAYTVWATIYDPLIRFLRRSRRRSLALLDIQPAESV
                     LILGCGTGADFEFLPRQAEVVAVDLTPAMLRRAAAKIGDRRIRLEEMDVMDLRFPRDT
                     FDKAILHLILAVVPDPVRALREAERVTKPGGLMVVLDKFWNHPGRPPLVLRMINAILG
                     GYVTAVNRNFHSIVSATCLEVVQEKPLGFGGLYYLYLLRKPGLPCDDSPVPLPRAWGD
                     RA"
     gene            2488..3291
                     /locus_tag="AC16G0_05870"
     CDS             2488..3291
                     /locus_tag="AC16G0_05870"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR5099186.1"
                     /translation="MTQHSLTGMLQGRKVFDLGMELYHGMPHHPMHSPFMYQMLRTHG
                     DLMYGEGVTAAVDIFGMSCHTGTHLDGLGHFAKNLCLHGGVPVDQGQSKTEGIRSCGI
                     QQVEPILRRGILLDVAATQGVEALGARQTIGPTELEAAAKAARVQPKPGDVVLVRTGW
                     IRHWADPRKYYSVQSGQPGLTADGARWITDQGVFCIGSDNFAVEYVPSPENTMPVHVH
                     CLVEKGVHLLEVVNLEELARAKTHEFLLIITPMKIRGGTASPVRPLAVV"
     gene            complement(3394..4752)
                     /locus_tag="AC16G0_05875"
     CDS             complement(3394..4752)
                     /locus_tag="AC16G0_05875"
                     /inference="COORDINATES: protein motif:HMM:NF013871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0052929 - ATP:3'-cytidine-cytidine-tRNA
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIR5099187.1"
                     /translation="MFRLFDLFKQTGYELYLVGGAVRDHLLGMALEDLVDVDFATSAL
                     PAESARILRAAGLPVFTVGSRFGTVGTVLEEGGSRKEVQVTTFRGEVYLNGSRKPRVS
                     FGKDLEVDLSRRDFSINAMAMTRDRRLVDPYGGEKDLEQRILRTVGDPHTIFREDPLR
                     TLRAARFIATLGMHPEPEVTRIVRELADDILTVSRERWLMEMNKLLPGVTVELALSFL
                     ADTGLLARLLPEAQAMVEFKAGQGRHHHKALWPHTTAVVGQAPARVAVRWAALLHDAG
                     KVTTRSVDTAGEVHFFGHEAVGASLVDDVARRFRFPQALHQQVHTMVLMHQRPALYEH
                     GWTDGAVRRLIRDAGDVLTDLLDLSRADVTSHRPGVREAVLERLAELEARAAELREKD
                     GQGPLLPKGIGLAIMQHFGLEAGPQVGELKGCLEQAVLDGELPRDGAAPEYLAYLDRV
                     RG"
BASE COUNT          972 a         1625 c         1604 g          884 t
ORIGIN      
        1 agaagcgcca acgaaaagtt gcggggaggt tgcttcccgt aggggcacat ctggagtaca
       61 ataggccctg ggtgcttcgt ctaccccgga aaccacggct ttcgcaggtt ccacacatgc
      121 ccgggaaatg ggtcgcgacc caccccgaga aacgcgcggt ggcttgctga gtccttccgg
      181 agttccggga ggtccgtgaa ggaggaagaa acggctccac gctcatggga gtgtcaacgg
      241 ggagaacgtg ccccccaccg actggaggtg caccgtgtgc tggcgctgcg attcacacga
      301 caagaacgaa gatgatcggg cgattgacgc tgccctggaa gaggcggtcc gaacgcatct
      361 atcgcgctcg ccggtttctc ggcgaaccct cctgcgggcc ggcttgggtc tcctggccat
      421 gcctgctgcg caagtcctcc agtcctgcgc cacggtcccg gtgacgggtc gctcccagct
      481 tcgcctcgtc tccgaatccg aggaggcgca gctcggggtg accgcgtttc agcagttgcg
      541 ccaagaggaa gtccagaagg gtcgccttct cagcgaacgc gaggacccga ccagccacgc
      601 ctgggtcaag cgcgtgagcg atcggatcat ccgggcttca ggcttggcca acgtgtacaa
      661 ctgggactac atgctcatca atgcccccaa gacggtgaat gccgccgcca tctccggagg
      721 acgcatcatc gtgtatacag ggattctccc cgtggcggag tccgatgccg ggctcgccac
      781 cattctcggc cacgaagtcg gtcacgtgat ggcccatcac acagcggagc gtgtgtccca
      841 aagtcaattg gtcaatgtgg gtgcggcgct gacgggcgag attgccgggc cgctcctggg
      901 ggctgtggtg ccggcggcgg ctgagcttgg atatctattg ccttacagcc gagcccagga
      961 aagcgaagcg gaccacattg gtctgttgct catggctaag gccggatatg atccacggga
     1021 agcggtcgcc ctctggcagc ggatggatcg gagcaccggg ggatcgggcc ggacgccgga
     1081 attcctctcc acgcacccca acccgggaac gcggattgcc gccctcgagg gctggttgcc
     1141 ccaggcctgg ccctattacc aaaacaacag tctgccctta ccgaacctgc aatagcctcg
     1201 gcggcaggag ggaaagcagt cacaggcagg gctctcgcag cagtcaaacc aggctgctgc
     1261 tcacggctcg tggcagagga gggatttcac gacacggccc ggctcaagac ttcaagccgg
     1321 gccgtacttt ttcaggcaga ggccggttca gcttttccgt gaggtcttgg cgaagaaact
     1381 gccgatgagg gtctcaagct cgctggagcc gcaggaaggg caggcgggcg gcttttctcc
     1441 ccgttccttg atggtcatgg taacggtgaa ttccttctgg catttcgtgc ataagaattc
     1501 gtaggtcggc atggctcgtc ctcctttcca gggtcggccg acgatggccg caaactttct
     1561 ccgacctata atttagtccc cagggccttt tcttaccaga gccgcctggg ctgcgggcga
     1621 gctatctcag gcccgatccc cccaggcacg cgggaggggc acgggcgaat catcgcatgg
     1681 aagccccggc tttcgcaaaa ggtacaagta gtacaatccg ccgaagccca ggggcttctc
     1741 ctggacaacc tccaagcatg tggcgcttac gatgctgtga aagtttcgat tcaccgccgt
     1801 cacgtatcca cccaggatcg cgttgatcat ccgcaggacc agcgggggcc ggccaggatg
     1861 gttccagaac ttgtccagga cgaccatgag tccgcctggc ttcgtcacgc gctcggcctc
     1921 tcggagggcg cggaccggat ccggcaccac ggccaggatg aggtggagga tcgctttgtc
     1981 gaatgtgtct ctcgggaagc ggagatccat gacatccatc tcttccagac ggatccgccg
     2041 gtccccgatc ttggcggccg cccggcggag catggccggc gtcaagtcta cggcgacgac
     2101 ctcggcctgc cgcgggagga actcgaaatc tgcccctgtt ccgcacccga ggatgaggac
     2161 gctctccgcc ggctggatgt ccagcagggc gagggagcgg cgacggctcc ggcgaaggaa
     2221 gcgaatcagg ggatcgtaga tcgtcgccca gactgtgtag gcgcggcgca gaccaagatt
     2281 cgtagccatg aatgaaacgc tcggagtttg ttccgtaggt gcaccccgac gatagagagg
     2341 aaagacacgg aggtcaagcc agaagcccgt cagctcgatc ccccccatca gacgacttga
     2401 cttccccgca tgcctgccta caatcggccg cacgattgct cgggattccc agaaggctgt
     2461 tcactactct aggcaccgga gggaccgatg acccaacact ctctgacagg aatgcttcaa
     2521 ggacggaagg tcttcgatct gggaatggaa ttgtatcacg gcatgccgca ccatccgatg
     2581 cactccccct tcatgtacca gatgctccgg acccatggag acctcatgta cggggagggc
     2641 gtgacggcag ccgtggacat cttcgggatg agctgccaca cgggcacgca cctcgacggt
     2701 ttggggcact ttgcgaagaa tctgtgcctg cacggcggcg tcccggtgga tcaggggcag
     2761 agcaagacgg aggggatcag gagttgcggt atccagcagg tggagcccat cctgcgcagg
     2821 ggcatcctct tggatgtggc agcgacccag ggcgtcgagg cgctgggagc ccggcaaacg
     2881 atcggcccga ccgaattgga ggccgccgcc aaggccgcca gagtgcagcc gaaaccgggt
     2941 gatgtcgtcc tggtccgcac gggttggatc aggcactggg ctgacccacg gaagtattac
     3001 agtgtccagt cggggcaacc cggcctcacc gccgatggcg ccaggtggat cacggatcag
     3061 ggggtcttct gcatcggcag cgacaacttc gcggtcgagt atgtcccttc accggagaac
     3121 accatgcccg tgcacgtcca ctgcctggtg gagaagggag tgcacctgct cgaggtggtg
     3181 aatctggagg agctggccag ggccaagacc catgaattcc tgctgatcat aactcccatg
     3241 aagatccgag gcggcaccgc cagcccggta cgaccgctgg ctgtggtgta ggtgaagcca
     3301 tgggagcgag atgagcagcg atatgggggc gagaccctat ggcaagcatc tcgaagaggt
     3361 cccgacgcca agcatgggga acgagtctga gcatcaaccc cggacgcgat ccaggtaggc
     3421 gagatactcg ggcgcggcgc catcccgagg gagttctcca tccagcaccg cttgctccag
     3481 gcaccccttc aactcgccca cctgcggacc ggcctcgagg ccaaagtgct gcatgatggc
     3541 caggccgatg cccttgggca ggagtggtcc ctgtccgtcc ttttcccgaa gctccgccgc
     3601 gcgcgcttcc agttccgcca ggcgttccag cacagcctcg cgaaccccgg ggcggtgaga
     3661 ggtgacatcg gcgcggctga ggtccagaag atcggtcaag acatcgcccg cgtcgcgtat
     3721 gagccgtcgg acggccccgt ccgtccaccc gtgttcgtac agcgccggcc gctggtgcat
     3781 cagcaccatg gtgtggacct gctggtgcag cgcctgcggg aagcggaagc gccgggccac
     3841 gtcgtccacc agcgacgcgc cgaccgcctc gtgcccgaaa aagtggacct cgccggccgt
     3901 atccaccgat cgggtcgtga ccttgccggc atcgtggagc agcgccgccc agcgaacggc
     3961 gacgcgcgcc ggcgcttggc ccaccacagc cgttgtgtgt ggccagaggg ccttgtggtg
     4021 atgccgtccc tggcccgcct tgaattccac catggcttgg gcctcgggaa gcaggcgcgc
     4081 gaggaggcca gtgtcggcaa ggaagcttag ggccaactca accgtaaccc ccggcagtag
     4141 cttgttcatc tccatgagcc atcgttcccg gctgaccgtc aggatgtcgt cggccagttc
     4201 ccgcacaatc cgggtgacct ccggctccgg gtgcatcccc agggtggcga tgaagcgggc
     4261 cgcgcggagg gtgcggagcg gatcctcgcg gaagatcgta tggggatccc ctaccgtccg
     4321 gaggatgcgt tgctccagat ccttctcgcc gccgtaggga tcgacgagcc ggcgatccct
     4381 tgtcatggcc atggcattga tcgagaaatc ccgccggctc aggtccactt ccagatcctt
     4441 tccgaagctg acccgaggct tccgactgcc gttgaggtaa acctcgcctc ggaacgtcgt
     4501 cacctggacc tcttttcgag atcccccctc ctcgagcacg gtcccgaccg tcccgaaccg
     4561 actccccacg gtgaagaccg ggaggcccgc cgcccgcagg atccgggcgc tctccgccgg
     4621 caacgcgctg gtcgcgaaat ccacatcgac caaatcttcc aacgccatgc cgaggagatg
     4681 gtcgcgtacc gcccccccta cgaggtaaag ttcgtacccc gtctgcttga acagatcgaa
     4741 cagtcggaac accttgaatt accccagacc gcagcccggt cgcgtccttc gccgacagag
     4801 gccgtgcacg atggtccgcc caatgaaaaa gccccaaggc ctttgggcct cagggcttac
     4861 gaggatccga atccgggacc tggaagcgcc taagaccctc cccccaccgg cggatgatgc
     4921 atggtcgaag agatagtcag catgggtgca tggctatccc ctccgttccc aggtcctcgt
     4981 gtgccgtctg aatcgtgctg ccaaccgcga tccataccct acccttcctc aaacgagttc
     5041 aaatatcaag caggccacgt ttcttgtcaa gcggaaatgc cgagc
//
LOCUS       DBNGZC010000325         2594 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2080687, whole genome shotgun sequence.
ACCESSION   DBNGZC010000325 DBNGZC010000000
VERSION     DBNGZC010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2080687"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            477..>2594
                     /locus_tag="AC16G0_05880"
     CDS             477..>2594
                     /locus_tag="AC16G0_05880"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020733384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HIR5099188.1"
                     /translation="MFAGLLALALSACATAARASEAELKIPDLGSVSFVGVSGPNLLL
                     FGLIVCVGGMAFGLVQYMQIRKLPVHRVMLEISELIYETCKTYLITQGKFLAILWAFI
                     AIVIVVYFGFLLRFSLGQVTTIVIFSIVGILGSYAVAWFGIRINTFANSRTAFASLGG
                     KPFPTMAIPLKAGMSIGMLLISVELVVMLFILLFVPGDSAGPCFIGFAIGESLGAAAL
                     RIAGGIFTKIADIGSDLMKIVFKIKEDDARNPGVIADCTGDNAGDSVGPTADGFETYG
                     VTGVALITFILLAVKDPTVQIQLLVWIFVMRVGMIVTSAASYWLNGLWAKAKFAEAAK
                     FNFEIPLTTLVWLTSIVSIGMTYVLSAVLIPELSGDSTLWWKLSTIITCGTIAGALIP
                     ELVKVFTSTNSRHVREVLSASREGGASLNILSGIVAGYFSAYWMGLAIILLMSIAYGV
                     STFGLGSLMAAPAIFAFGLVAFGFLGMGPVTIAVDSYGPVTDNAQSVYELSTIETLPN
                     VKKEIEKEFGFPPDFENAKAFLEENDGAGNTFKATAKPVLIGTAVVGATTLIFSIIQV
                     LTKMFGEADVSTGLSILNPLFLLGLITGGTVIFWFSGASIQAVSTGAYRAVEFIKRNI
                     KLEGGGERASIEDSKKVVAICTQYAQKGMFNIFVAIFFSTLAFACVNHYYFIGYLISI
                     AIFGLFQAIFMANAGAAWDNAKKL"
BASE COUNT          428 a          882 c          762 g          522 t
ORIGIN      
        1 tgcgtaacgg aaggatagac ccggccccag gggctgtcaa tgggaaaggg tcaagggagg
       61 cggggaaacg gcggtgaatc agcccctccc cccacggccg tcggacgggc cagaccgacc
      121 tcggccctct ccggaaagcc gcacccagcg cggtccggaa ggaccgaccc cccgcacatt
      181 cggcttgaca catgctgacc gccttggtac gttcctcccg cccatgagat cgatggtcgc
      241 cgctggttcg gcctggcgac agtcggaagt acggtctcct gccggacaat accaactatg
      301 ctaaacggcg ggagatccat tcccgctggg gatcactcac cgtttctctt attgaccaat
      361 cttatctacc cgggaggggc tgcgaggagg gtggcatcgt gagcgaagcc agtatcctgc
      421 ttccgtttct tggtccgggc actctcattc gccgtgcctt ggtggcatca cccaggatct
      481 tcgcaggcct tttggcgctg gcgctgtctg cctgcgccac tgctgccagg gcgagcgagg
      541 cagagctgaa gatccccgac ctgggcagcg tgagcttcgt cggggtctcc gggcccaacc
      601 tgctcctgtt cgggctcatc gtctgcgtcg gcgggatggc cttcgggctt gtccagtaca
      661 tgcagatccg caagctcccg gttcaccggg tgatgctcga gatctccgag ctcatctacg
      721 agacctgcaa gacctacctg atcacccagg gcaagttcct ggcgatcctc tgggcgttca
      781 tcgcgatcgt catcgtggtc tatttcggat tcttgctccg cttctcgctg ggtcaggtga
      841 ccaccatcgt gatcttcagc attgtcggca tcctgggcag ctacgccgtg gcgtggttcg
      901 gcattcggat caacaccttc gccaactccc ggacggcctt cgccagcctt ggcggcaagc
      961 ctttcccaac catggccatc ccgctcaagg cggggatgag catcggcatg ctgctcatct
     1021 cggtcgagct cgtggtcatg ctcttcatcc tcctcttcgt ccccggcgac tcggcgggtc
     1081 cctgcttcat cggcttcgcc atcggtgagt ccctgggcgc cgcggccctc aggatcgccg
     1141 gcggcatctt caccaagatc gccgacatcg gctccgacct gatgaagatc gtcttcaaga
     1201 tcaaggagga cgacgcgcgg aaccccggcg tcatcgccga ctgcacgggc gacaatgccg
     1261 gcgattctgt cggcccgacc gcggacggct tcgagaccta cggcgtcacg ggggtggccc
     1321 tgatcacctt catcctgctg gcggtgaaag accccaccgt ccagatccag ctcctggtct
     1381 ggatcttcgt gatgcgcgtc ggcatgatcg tgacgagcgc cgcgtcctac tggctgaacg
     1441 gcctctgggc caaggcgaag ttcgccgaag ctgccaagtt caacttcgag attcccctga
     1501 ccacgctggt gtggctcacc tcgatcgtgt ccatcggcat gacctacgtc ctgtcggccg
     1561 tgctgatccc cgaactgagc ggcgattcca cgctctggtg gaagctctcc accatcatca
     1621 cctgcggcac cattgccggc gccctgatcc ccgagctggt caaggtcttt acctcgacga
     1681 actcccgcca cgtccgtgag gtcctctcgg cctcccgtga ggggggcgcc tccctcaaca
     1741 tcctgagcgg catcgtggcc ggctacttca gcgcctactg gatgggcctg gcgatcatcc
     1801 tcctgatgtc catcgcttac ggcgtgagca ccttcgggct cggcagcctc atggcggctc
     1861 ccgcgatctt cgccttcggc ctggtggcct tcggcttcct gggcatgggt ccggtgacga
     1921 tcgccgtgga ctcctacggc ccggtgacgg ataacgccca gtcggtgtac gagctgtcca
     1981 ccatcgagac cctccccaac gtgaagaagg agatcgaaaa ggagttcgga ttcccgcccg
     2041 acttcgagaa cgccaaggcc ttcctggagg agaatgacgg cgcgggcaac accttcaagg
     2101 ccacggccaa acccgtgctc atcgggactg cggtggtggg cgcgacgacc ctgatattct
     2161 ccatcatcca ggtgctcacg aagatgttcg gggaggccga cgtctcgacc ggcctgtcca
     2221 ttctgaaccc cctcttcctc ctgggcctca tcaccggcgg cacggtcatt ttctggttct
     2281 ccggcgcctc catccaggcc gtctccaccg gcgcctaccg cgccgtggag ttcatcaagc
     2341 gcaacatcaa gctcgagggc ggcggggagc gggcctccat cgaggacagc aagaaggtgg
     2401 tcgccatctg cacccagtac gcccagaagg ggatgttcaa catcttcgtg gcgatcttct
     2461 tctcgaccct ggccttcgcc tgcgtgaacc actactactt catcggctac ctgatctcga
     2521 tcgcgatctt cggcctgttc caggcgatct tcatggcgaa cgccggtgcc gcctgggaca
     2581 acgccaagaa gctc
//
LOCUS       DBNGZC010000326         2562 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1885721, whole genome shotgun sequence.
ACCESSION   DBNGZC010000326 DBNGZC010000000
VERSION     DBNGZC010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1885721"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..886)
                     /gene="soxA"
                     /locus_tag="AC16G0_05885"
     CDS             complement(116..886)
                     /gene="soxA"
                     /locus_tag="AC16G0_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011647469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016669 - oxidoreductase activity, acting
                     on a sulfur group of donors, cytochrome as acceptor
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019417 - sulfur oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxA"
                     /protein_id="HIR5099189.1"
                     /translation="MLLAFSEVAVPGQGTSLPTGIRPEERRSGYFFLSRETQAMQDDD
                     QANPGMLWVQEGSSLWGKAEGASGKACASCHQDAAVSMKGVAARYPAFDAGAGRVINL
                     EQRINLCRVERQQAPPLPYESEELLALTTYVAHQSRGLPIRVAIDGPARPFFEAGRTF
                     FLTRRGQLNLACNQCHEDHWGQKLRSERISQGYGNAYPAYRLEWQTIGSLQHRLRNCN
                     VGVRAEPYPYGAQEYVNLELYLAWRGQGLPMETPGVRR"
     gene            complement(922..1242)
                     /gene="soxZ"
                     /locus_tag="AC16G0_05890"
     CDS             complement(922..1242)
                     /gene="soxZ"
                     /locus_tag="AC16G0_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate oxidation carrier complex protein
                     SoxZ"
                     /protein_id="HIR5099190.1"
                     /translation="MAKALIRVPPEVKKGEVFEIKTLISHPMEPGQRRDNMGRLIPRD
                     IINRFICTYNGDEVFRADFFPAIAANPFLTFFTIAMESGELIFQWTDDHGVTQTDSAK
                     ITVV"
     gene            complement(1253..1732)
                     /locus_tag="AC16G0_05895"
     CDS             complement(1253..1732)
                     /locus_tag="AC16G0_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxY-related AACIE arm protein"
                     /protein_id="HIR5099191.1"
                     /translation="MSCNVPEPAVQVTRRQFLGAVILGTALPLTLRRAAEASPETAAA
                     AMKAILGDAPLREGKVKLDIPPIAENGNSVPLTVSVDSPMTAADHVRSIHIFAEENPL
                     PNVAHFYLGPRAGKAQVSTRIRLFKSQTILAVAALRDGTFWSAKADIVITLAACIED"
     gene            complement(1762..2205)
                     /gene="soxX"
                     /locus_tag="AC16G0_05900"
     CDS             complement(1762..2205)
                     /gene="soxX"
                     /locus_tag="AC16G0_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur oxidation c-type cytochrome SoxX"
                     /protein_id="HIR5099192.1"
                     /translation="MQRATAATVAVVLSGVSALPAEPLRPFVVVGDAIPESLTGQPGD
                     PQRGREVVMSRELGNCLLCHRMPIPEERFQGNIGPDLSGVGGRLSGGQIRLRIVDSSR
                     LNPTSIMPPYYRVEGLQRVLTAYRDKPVLTAEQVEDAVAFLSALR"
     gene            complement(2237..>2562)
                     /locus_tag="AC16G0_05905"
     CDS             complement(2237..>2562)
                     /locus_tag="AC16G0_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FCSD flavin-binding domain-containing protein"
                     /protein_id="HIR5099193.1"
                     /translation="GDAAIAGAMPKSAFAANAQAKICAAAVATLLRGGVASDPVLVNT
                     CYSLVAPDYGISVAGVYRPKEGQLADVEGAGGTSPLQAGQETRALEARYAEAWFKTIT
                     DEVFG"
BASE COUNT          417 a          864 c          829 g          452 t
ORIGIN      
        1 cgaacttctc caggaagaac ctctcggatg gttgcgtctc ttgggtcatg atcagttcat
       61 tctcctcggc ctgtaatcat tccgcgggcg attcaccgcc cgccgtcgcc ggcccttatc
      121 gtcgaacccc cggcgtttcc atcggcagcc cctggcccct ccacgcgagg tacagctcca
      181 ggttcacgta ttcctgagcc ccgtacggat agggctccgc gcgcaccccg acgttgcagt
      241 tccgcaggcg gtgctgcagc gacccgatcg tttgccactc cagccggtag gccgggtagg
      301 cattgccgta gccttgcgag atccgctcgc ttcgcaactt ctgcccccag tgatcctcgt
      361 ggcactggtt gcaggccagg ttgagctgcc cccgccgcgt caggaaaaag gtgcgccccg
      421 cctcgaaaaa cgggcgggcg ggtccgtcga tggcgacccg gatcggcagg ccgcgggact
      481 gatgcgccac gtaggtcgtc agcgccagga gctcttcgga ctcatacggg agcggcggtg
      541 cctgctgccg ttccacccga cacaagttga tgcgttgctc cagattgatg actcggcccg
      601 ccccggcgtc gaaggccggg tagcgtgccg ccaccccttt catgctgacg gccgcgtcct
      661 ggtgacagct cgcacaggcc ttcccggacg cgccctccgc ctttccccac agcgaactgc
      721 cctcctgcac ccacagcatg cctgggttgg cctggtcatc atcctgcatc gcctgcgtct
      781 ccctcgacag gaagaagtat ccggagcgtc tctcctccgg gcggattccg gtcggcaggc
      841 tcgtgccctg gccgggcacc gcgacctcag aaaacgccag aagcatcgcc gggaggcaag
      901 ctgaccagct gagacgagac attatacgac cgtgattttg gcgctgtcgg tctgggtgac
      961 gccgtggtcg tcggtccact ggaaaatcag ttcgcccgac tccatggcga tggtgaagaa
     1021 cgtgagaaag ggattggcgg cgatggccgg aaagaaatcg gcccggaaaa cctcgtcgcc
     1081 gttatacgtg cagatgaacc ggttgatgat gtcgcggggg atgagccggc ccatgttgtc
     1141 gcgtcgctgg ccgggttcca tggggtgaga gatcagggtc ttgatctcga acacctcgcc
     1201 cttcttcacc tcggggggta cgcggatgag cgccttggcc atggcccgtt cctcagtctt
     1261 cgatgcaggc ggcaagcgtg atgacgatat ccgccttggc ggaccagaag gttccgtcgc
     1321 ggagcgctgc cacggccagg atcgtttgcg atttgaagag tcgaattcgc gtcgagacct
     1381 gggccttgcc cgcgcggggg ccgaggtaga agtgcgcgac attcggcagc gggttctcct
     1441 cggcgaagat gtggatgctc cggacgtggt ctgccgcggt catcgggctg tcgacgctga
     1501 ccgtgagcgg caccgagttg ccgttctcgg cgatcggggg aatgtccagc ttgaccttgc
     1561 cctcgcgcag gggggcgtcg cccaggatgg ccttcattgc cgccgctgcg gtctcggggc
     1621 tcgcctccgc ggcgcggcgt agcgtcagcg gcagtgccgt tcccagaatg acggccccaa
     1681 ggaactggcg gcgggtcacc tggacggctg gctcgggaac gttgcaggac atccttatcc
     1741 tcagtttctg cgagacatcc ttcagcgaag cgccgagaga aaggccacag catcctcgac
     1801 ctgctccgcc gtgagcacgg gcttgtcccg gtaagcggtc aggacccgct gcaggccctc
     1861 cacccggtaa tagggcggca tgatgctggt ggggttcagc cgtgacgaat ccacaattcg
     1921 cagccgaatc tgcccgcccg acagccggcc cccgaccccg gaaagatcgg gcccgatatt
     1981 cccctgaaac cgttcctcgg gaatcggcat ccggtggcag aggaggcaat tgccgagctc
     2041 acgactcatc acgacctccc gcccgcgttg cgggtcgccc ggctggccgg tcaaagactc
     2101 cgggatcgcg tccccgacca ccacaaaggg tcggagcggc tccgccggca gggcagaaac
     2161 gcctgacagg accaccgcca cagtcgccgc cgtggctcgc tgcaaccagc ctcgaactcg
     2221 cggcagtccc accgcgctaa ccgaagacct catccgtgat ggtcttgaac caggcctcgg
     2281 cataccgggc ctccagcgcg cgggtctcct gtcccgcctg gagcggactt gtcccaccgg
     2341 ccccttcgac gtcggcgagc tggccctcct tggggcggta gacccccgca acggagatcc
     2401 cgtaatccgg tgcgaccagg ctgtagcagg tgttcaccag gacgggatcc gacgccacgc
     2461 cgccccgcag cagcgtggcc accgcggcgg cgcagatctt ggcctgggcg tttgcggcga
     2521 aggcggattt tggcatggcg ccggcgatgg cggcgtcgcc ga
//
LOCUS       DBNGZC010000327         3246 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_907336, whole genome shotgun sequence.
ACCESSION   DBNGZC010000327 DBNGZC010000000
VERSION     DBNGZC010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_907336"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..767)
                     /gene="ilvN"
                     /locus_tag="AC16G0_05910"
     CDS             complement(234..767)
                     /gene="ilvN"
                     /locus_tag="AC16G0_05910"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR5099194.1"
                     /translation="MRHVLVALVQDEPGMLARMGNLFARRGYNVESFTAGASELPGLT
                     RITIVVDGQSSALDQVAKQFRKLIGAVKVADVTADKDQLAHEMLMVKVGVTAKTRQEV
                     VGLADLFKARIIDVSTTTLMLEATGEPDKLDKLLEVLRPFGVKEQARTGAVFMSRGAK
                     VLNQRPHSASDRKYLTE"
     gene            complement(764..2452)
                     /gene="ilvB"
                     /locus_tag="AC16G0_05915"
     CDS             complement(764..2452)
                     /gene="ilvB"
                     /locus_tag="AC16G0_05915"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HIR5099195.1"
                     /translation="MRFTGAEVLWECLVREGVNVVWAYPGGAALPLFDALAKYPQIHH
                     VLVRHEQAAAHAADGYARATGKVGVCVATSGPGATNLVTGIATAIMDSVPIIAVTAQV
                     ADSVIGTDAFQEINITGITLPITKHNYLVRQSRDLARTLREAFFLARTGRPGPVLVDV
                     PKDILTAPCEFHYPERISRPGYRPVVRSAPYQIQRAAQLIADAQRPVVLAGHGVHVAT
                     AWEELRRFAERINAPVVSTLLGLSALSADHPLCLGMAGMHGVAWANLAVQNADLLIAL
                     GMRMDDRFTGSLPHFAPQAKLLHVDIDPAEIGKRVPAFLPMVGDVRLVLEAVTAALPE
                     LDHRDWLAQIETYRQRHPLDVHAQGPLPPQEVIRALHEATEGEGVLVSDVGQHQMWVA
                     QHYRFRRLNSFFTSGGLGTMGYSLPAAMGVQVAHPKEPVWVVVGDGSFQMNLQELATV
                     VQERLPLKIVIMNNGYLGMVRQWQELFHGARYIGTPISGPDFVQLAQSYGIPGRRIGT
                     ADELKTAFMAARETDGPYLLDCRVVEDENVYPMVRPGTSNTDFVEDPRRRTQES"
     gene            2718..>3246
                     /locus_tag="AC16G0_05920"
     CDS             2718..>3246
                     /locus_tag="AC16G0_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099196.1"
                     /translation="MRKVFESFCFILVVSLLVSPQAIPAAETIKLNFLEVMTSPGRTA
                     VVKELIERFQAGHPNISINLISPPYEQSDNKLTMMMNAREPLDIVEVRELTQKQYINN
                     GLLENLESRLKSWADAKTLLPVTLEAARYVDNTAYMIPESIYVKALFMRTDVLKKLGV
                     EAAPTTIQQLYDLAKK"
BASE COUNT          590 a         1026 c         1061 g          569 t
ORIGIN      
        1 aagaacatga cgaccaagaa ggagaggagg acccccggaa tggggcgaat agcctgggac
       61 aggtcggacg ggcgggcgga tgaaccgttg gtccggtcgg tcatggtaga gcgaggactt
      121 ggacgagtgg catggcgtgt gcgaactcca gggccgttgc cggacgtggc gctacacctt
      181 ggctaccccg catccgccca ggggtggcga ggcagatcag gcgtcggcgc ccgtcactcc
      241 gtcaggtact tccggtctga ggcggagtgg ggacgttggt tgaggacttt ggcgccgcgg
      301 ctcatgaaga cggcgccggt gcgggcctgc tccttgacgc cgaaggggcg cagcacttcg
      361 aggagtttgt ccagcttgtc cggctccccc gtcgcctcca gcatcagggt ggtcgtggag
      421 acgtctatga tgcgggcctt gaacaggtcg gcgaggccaa ccacctcctg ccgggtcttg
      481 gcggtcaccc ccaccttgac catcagcatc tcgtgggcca attggtcctt gtcggcggtc
      541 acgtctgcca ccttgaccgc gccgataagc ttccggaact gcttggccac ctgatccagg
      601 gcgctgctct gaccatccac cacgatggtg atccgcgtga gtccgggcag ctccgacgcg
      661 cctgccgtga agctctccac gttgtagccg cgccgcgcga aaaggttccc catgcgggcc
      721 agcatgcctg gctcgtcctg gaccagcgcc accaagacgt gtctcatgac tcctgcgtcc
      781 tcctccgcgg gtcctccacg aagtcggtat tactggttcc cggccggacc atggggtaca
      841 cgttctcgtc ctcgaccacc cgacagtcca ggaggtatgg gccgtcggtc tcccgcgcgg
      901 ccatgaacgc cgttttgagc tcgtctgcgg tgccgatgcg ccggcccggg atgccgtagc
      961 tttgagccag ttgaacgaag tccgggccgc tgatgggcgt gccgatgtag cgggccccgt
     1021 gaaacagctc ctgccactgc cggaccatcc ccaggtagcc gttgttcatg atcacaatct
     1081 tgaggggaag tcgctcttgg acaacggtcg ccagctcctg caggttcatc tggaagctgc
     1141 cgtcgccgac gacaacccag acgggctcct ttgggtgtgc aacctgcacg cccatggcgg
     1201 cgggcagcga atatcccatg gtgccgagac cgccggaggt gaagaagctg ttgagacgcc
     1261 ggaaacggta gtgctgggcc acccacatct ggtgctggcc gacatcgctc accaggacgc
     1321 cctcgccctc ggtggcctcg tggagtgccc ggatgacctc ctgcgggggc agggggccct
     1381 gggcgtgcac gtccagggga tggcgctggc gataggtctc gatctgggcc agccagtccc
     1441 gatggtcaag ctcgggcagt gccgccgtca cggcttccag caccaggcga acatcgccca
     1501 ccatgggcag gaaggcgggg acgcgcttgc cgatctcggc ggggtcgatg tccacgtgaa
     1561 gcagcttcgc ctggggcgcg aagtgcggca gactcccggt gaagcggtcg tccatgcgca
     1621 tccccagggc gatgagcaga tcggcgttct gcacggccag attggcccag gccacgccgt
     1681 gcattcccgc catgccgaga cagagcggat ggtccgccga gagcgcgctg agaccgagga
     1741 gggtgctgac caccggggcg ttgatccgct cggcgaagcg ccgcagctct tcccaggctg
     1801 tggcgacgtg cacgccgtga ccggcgagca ccacgggacg ctgtgcgtca gcgatcagtt
     1861 gcgcggcccg ctggatctgg tagggcgccg agcgcacgac cggacggtag cccggccgtg
     1921 agatccgctc ggggtagtgg aactcgcagg gggcagtcag gatgtccttg ggcacgtcca
     1981 ccagcaccgg gccgggccgt ccggtcctgg ccagaaagaa ggcctcgcgc agtgtccgcg
     2041 ccagatcccg actctgccgc accagataat tgtgcttggt gatgggcagg gtgatgccgg
     2101 tgatgttgat ctcctggaag gcgtcggtgc cgatcaccga atcggcgacc tgcgccgtca
     2161 cggcgatgat gggcaccgag tccatgatgg ccgtcgcgat cccggtaacc agattcgtgg
     2221 cgccgggccc ggacgtcgcc acgcagacgc ccaccttgcc cgtggcgcgg gcatatccgt
     2281 ccgccgcatg ggcggccgcc tgctcgtggc gcaccaggac gtggtgaatc tgggggtact
     2341 tggcgagcgc atcgaacaac ggcagggcgg ccccgcccgg ataggcccag acgacgttca
     2401 cgccctcgcg gaccaagcat tcccacagga cctcggcacc agtgaatcgc atgctcggat
     2461 gctgccccct cccgctcgac gtgtcttcgg tgacggggac cataccagcg gtcggaagga
     2521 cggtcaagcc gttttcggcc gccagggtag gatggtgtgc cttttcgtca aataccacga
     2581 caatgtctcg ataatccgtc aattgtccct atcgtcttcg cggtcatttg acaatagagg
     2641 cagtctcgca tgagaatgca tcccatttca attcagacag ggcaggaaga atgacgttcc
     2701 atcggaggag ggaacccatg agaaaagtgt tcgagtcgtt ctgcttcatc ctcgttgtga
     2761 gtcttctggt atccccgcag gcgatccctg ccgccgaaac gatcaaattg aatttcctcg
     2821 aagtcatgac gagcccgggc cggactgccg tcgtgaagga gttgatcgaa agattccagg
     2881 caggccaccc caacatcagc atcaacctga tctctcctcc gtacgagcag tccgacaaca
     2941 aactcaccat gatgatgaat gcccgcgagc ccctcgacat cgtggaggtt cgagagttga
     3001 cgcaaaaaca gtacatcaac aacggactgc tggagaatct ggagtcgcgc ctgaagagct
     3061 gggcggatgc caagaccctg ctgcctgtga ccctcgaggc cgctcggtac gtggacaaca
     3121 cggcctacat gatccccgaa tcgatctacg tcaaagcgct cttcatgcgc accgacgtct
     3181 tgaagaagct gggggtggag gcggcgccga ccaccatcca gcaactctat gacctggcga
     3241 agaaag
//
LOCUS       DBNGZC010000328         2420 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1004997, whole genome shotgun sequence.
ACCESSION   DBNGZC010000328 DBNGZC010000000
VERSION     DBNGZC010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1004997"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..2179
                     /locus_tag="AC16G0_05925"
     CDS             98..2179
                     /locus_tag="AC16G0_05925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR5099197.1"
                     /translation="MVREIRKTVCPHDCPDTCSILATVEDGQVIGCEGDPDHPFTRGG
                     LCHKVNRYAERIYSPSRVLYPMRRTGKKGEGKFVRITWDEALQEVAHRLEAVARDYGG
                     EAILPFSYGGTLGLVQRKAGHAFFHRLGATRLKRNICDTAAEEAWRATYGACLGTDME
                     GIEASDLVILWGINAVHTNVHGMRFVMRARQNGARLVVIDPYRNRTAKLADTHLMLRP
                     GTDGALALGLAHVLVRDGLLDREYIQRRTFGFDVYRQEVEQYPPGRVAEITGIPRAAV
                     EDLARAYGQARAPFIRVGNGLQRHTNGGQAIRAIACLPGLTGAFERPGGGALWETFDA
                     FHTHFAAIEAEDLQPHPTREVNMVQLGDTLLRLDRPPVKALFVYQANPAANIPDQRQV
                     RAGLLREDLFTVIHEQMHTDTADFADVLLPATTSFEHLDLYRSYGHYHLQLGRPVIPP
                     LGEARSNLELFQALAETLGFTHPIFRRTTQELIQDLLRVDHPHVAGITPERLASGDPI
                     RVNIPRLGDPFAGVFPTPSGKLEFFSQSLAARGLPALPTYVPAVEGHEHRTPKFPLQL
                     MTPPSKDFLNTSFGCVERMVRSEGKPRLKIHPTDARSRGIAQDVLVRVYNDRGECFLY
                     AEVTGDVPPGVLVAEAIWWAKHHPGGKGINQLTSQRLTDLGECSTLHENLVNVAVADA
                     DAILLSGSKRQ"
     gene            2176..>2420
                     /locus_tag="AC16G0_05930"
     CDS             2176..>2420
                     /locus_tag="AC16G0_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5099198.1"
                     /translation="MNLFQRFEKVAAGQPDRLALQIKEAAGYRRLTYGEILRQALALA
                     AALIRAGIAQGDRVALLCENRPEWAVAYFGLTAAGAVA"
BASE COUNT          404 a          827 c          778 g          411 t
ORIGIN      
        1 gcgtcctccg ggcggcactg aggccctgcg ttatgcggcg aatctctggg acaggacact
       61 agcattccga ttatcgatta gagattgagg tcgccggatg gttcgtgaga tccgcaaaac
      121 ggtgtgcccg cacgactgcc ccgacacgtg cagcatcctg gccaccgtcg aggatggtca
      181 ggtgatcggg tgcgagggag accccgacca tcccttcacc cgaggcggcc tgtgtcacaa
      241 ggtgaaccgg tacgccgagc ggatctattc gcccagccgg gtgctttacc ccatgcgtcg
      301 tacggggaag aagggggaag gcaagttcgt ccgcatcacc tgggacgagg ccctgcagga
      361 agtcgcccat cgtctggagg ccgtggcgag ggactacggc ggcgaggcca ttcttccttt
      421 ctcctacggc ggaacgctgg gcctggtcca gcggaaagcg ggccacgcct tcttccaccg
      481 cctgggggcc acccgcctga agcggaacat ctgcgataca gccgccgagg aggcctggcg
      541 cgcgacctac ggcgcctgtc tcgggacgga catggagggg atcgaggcat cggacctcgt
      601 gatcctctgg gggatcaacg ccgttcacac caatgttcac gggatgcgct tcgttatgcg
      661 ggccaggcag aacggcgccc gcctggtggt gatcgatccc taccgcaacc ggacggccaa
      721 gctggcggac acccatctca tgctccggcc cggcaccgac ggcgccttgg ctctcgggct
      781 cgcccatgtc ctcgtccggg acggcctgct ggaccgggag tacatccagc ggcggacctt
      841 cggcttcgat gtctaccggc aagaggtcga gcagtacccg ccagggcgtg ttgcggagat
      901 caccggcatt ccgcgcgcgg ccgtcgagga tctggctcgg gcctacggcc aggcgcgcgc
      961 gcccttcatc cgggtgggca acggtctcca gcggcacacc aacggcggcc aggccattcg
     1021 ggccatcgcc tgccttcccg gcctcacggg tgccttcgag cggccggggg gtggggcctt
     1081 gtgggagacc ttcgacgcgt ttcacaccca cttcgcggcc atcgaggcgg aggatctcca
     1141 gcctcacccg acccgcgagg tcaacatggt gcaacttggc gacaccctgc tgcgcctgga
     1201 ccggccgccc gtcaaggccc tcttcgtgta tcaggccaac cctgcagcca acatcccgga
     1261 ccagaggcag gtccgggccg gtctcctgcg tgaggacctc ttcaccgtga tccacgagca
     1321 gatgcatacc gacacggcgg atttcgccga cgtgctcttg ccggccacga ctagcttcga
     1381 gcacctcgat ctgtaccgga gctacgggca ctatcatctg cagcttggcc gtcccgtcat
     1441 cccgcccctg ggggaggctc gatcaaattt ggagctgttc caggctctgg cagaaacact
     1501 ggggtttacc caccccatct tccgcaggac cacccaagaa ctgatccagg atctcctccg
     1561 ggtggaccat ccgcacgtcg cgggcatcac gccggagcgt ctggcctccg gcgacccgat
     1621 ccgggtgaac atcccgcgcc tgggtgatcc cttcgcaggc gtcttcccca ctccctccgg
     1681 caaactggaa ttcttctcgc agtctctggc agcccggggt ctccccgccc tgccgaccta
     1741 cgtcccggcg gtcgagggcc acgagcatcg cacgccgaaa ttcccgctgc agctgatgac
     1801 gccgccctcc aaggattttc tgaacaccag cttcggctgc gtcgagcgca tggtccggtc
     1861 cgaggggaaa ccacgactga agatccatcc cacggatgcc cggtcgcgag gcatcgccca
     1921 agacgtcctc gtgcgggtct ataacgaccg cggggagtgc ttcctgtatg ccgaggtgac
     1981 gggggacgtt ccccccggcg tcctggtggc cgaagccatt tggtgggcga agcaccaccc
     2041 gggcggcaag gggatcaacc agctcacctc gcagcggctg accgatctgg gcgagtgcag
     2101 caccttgcac gagaatctcg tgaacgtggc cgtcgccgat gccgatgcaa ttctgctgag
     2161 tggctcgaaa cgacagtgaa tctcttccag cgctttgaga aagtcgccgc cgggcagccg
     2221 gaccgactgg ctctccagat caaggaggct gccggctatc gccgcctgac ctacggcgag
     2281 atcctccggc aggccctggc gttggctgcg gcactcatcc gggcggggat tgcccaaggc
     2341 gatcgggtcg ccttgctctg cgagaaccgg ccggaatggg ccgtggccta cttcgggctg
     2401 acggcagccg gggccgtcgc
//
LOCUS       DBNGZC010000329         1600 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1298374, whole genome shotgun sequence.
ACCESSION   DBNGZC010000329 DBNGZC010000000
VERSION     DBNGZC010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1298374"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="AC16G0_05935"
     CDS             complement(<1..861)
                     /locus_tag="AC16G0_05935"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR5099199.1"
                     /translation="MLFGFRVVTFRLAIFVIVGLAQAYLFTGIRRAIRASKQSPRSQT
                     AMIWLSGFIISFMYVLNAYIVLWHLPWVDVPLVAQVGLLYPVAVWNFGSVFSAVVLLL
                     MRIAGRAGRLASRVWRLPTGDPVPAPVDMGRRRFLRAGAGSLAAAPLVLSGYGAAYAS
                     KAYGVEEVSLPFGRPLRVVQLSDIHAGLYMTREEMRRYADRVNELNPDLLVLTGDFIS
                     NSMSFLPGCAEEMARVRTRNGTFATLGNHEHWYGNLREIRAVFGRSGITLLNNAHQLI
                     QTDSGPFAIAG"
     gene            963..1418
                     /locus_tag="AC16G0_05940"
     CDS             963..1418
                     /locus_tag="AC16G0_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR5099200.1"
                     /translation="MRPEADVSKARLLRPAVAAIITNAEGRILLQRRSDNGLWGLPGG
                     SVEIGESVGQAILREVREETGLTVEVVRLIGVYSDPKFQVVRYPDDNVVHYISILFGC
                     RISGGTLQTCDETLDLGYFGPTELPSDLVPMHRIRIEDAVADRPTAFIR"
     gene            complement(1471..>1600)
                     /locus_tag="AC16G0_05945"
     CDS             complement(1471..>1600)
                     /locus_tag="AC16G0_05945"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF013494.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIR5099201.1"
                     /translation="RPAHHPDITLNYTRVTLSLSPHDAGGITEKDFDLAQRAEALL"
BASE COUNT          322 a          473 c          547 g          258 t
ORIGIN      
        1 ccccgcgatg gcgaaggggc ccgaatcagt ctggatgagc tggtgggcat tgttcaggag
       61 tgtgattccg ctccggccga acacggcccg gatctcgcgg aggttcccgt accagtgttc
      121 atggttcccg agggtggcga aggtgccgtt gcgggtgcga acccgggcca tctcctcggc
      181 gcagccgggg agaaatgaca tcgagtttga gatgaagtcc ccggtcagga ccaagagatc
      241 cggattcaac tcgttcaccc gatcagcata cctacgcatc tcctcacggg tcatgtacag
      301 gccggcatgg atgtcactga gctggacgac ccggagcgga cggccaaagg gcagagagac
      361 ctcttcgacg ccgtaggcct tgctggcgta agccgctccg taccccgaga gcaccagggg
      421 cgccgcggcc aggctgcccg caccggcccg caggaagcgg cgtctcccca tgtctaccgg
      481 ggccggcact ggatcaccag tcgggagacg ccagacccga gaggcgaggc gacccgcgcg
      541 gccggcgatt cgcatcagga gcagtaccac cgcggaaaac actgaaccga agttccacac
      601 cgccaccgga tagagcaggc cgacttgcgc gaccagcgga acatctaccc agggcaggtg
      661 ccagagcacg atgtaggcgt tcaggacgta catgaagctg atgataaagc cggacagcca
      721 gatcatggcg gtttgagagc gtggagactg cttggaggcc cggatggcgc gccggatgcc
      781 ggtaaacaga taggcctggg ccaagcccac gatgacgaag attgccaggc gaaaggttac
      841 gactcggaaa ccgaaaagca tgggggagac tccagtcaac gagtgattgg aacgacggag
      901 gataagagaa ctatccggga tgcgcaagca tcttcgtccg tacaccttgc aagggatggg
      961 acatgagacc tgaggccgat gtgagcaagg ccaggctgct ccggccggcg gtggcggcca
     1021 tcatcacaaa tgcggagggg agaatcctcc tgcaacgtcg ctcggataac ggcttgtggg
     1081 gcttgcccgg cgggtcggtc gagatcgggg agtcggttgg tcaggcgatc ctccgcgagg
     1141 tgcgcgagga gacgggtctg acggtggagg tggttcgctt gatcggggtg tactcggacc
     1201 cgaagtttca ggtggttcgc tacccggacg acaacgtggt tcactacatc agcatcctat
     1261 tcggctgccg gatctcaggc gggacgcttc agacctgcga cgaaacgctc gacttagggt
     1321 atttcggacc gaccgaactc ccgagcgacc tcgtgccgat gcatcggatc cggatcgagg
     1381 acgccgtcgc cgaccgcccc acggccttta tccgctagca gcccgcccga cggcgggccc
     1441 gtagcccctg cagccgaccc atcggccccc tcaaaggagc gcctctgctc gctgggcgag
     1501 atcgaagtct ttctcggtga tcccgccggc atcgtggggg gagaggctca gcgtgacgcg
     1561 agtatagttg agcgtgatgt cggggtggtg tgcgggtcgc
//
LOCUS       DBNGZC010000330         1579 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_418026, whole genome shotgun sequence.
ACCESSION   DBNGZC010000330 DBNGZC010000000
VERSION     DBNGZC010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_418026"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="AC16G0_05950"
     CDS             complement(<1..983)
                     /locus_tag="AC16G0_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR5099202.1"
                     /translation="MLIRRRCVLMGLAAFPFAASLGRIARAWAARPAPVDPQERHFRN
                     LRQLTFGGQNAEAYFAPDGKRLILQSTRDGRHCDQIYIMDLEGNDVRMVSTGKGVTTC
                     SFLFPDGRRFIYSSTHLAGPECPPRPDMSRGYAWALQPYHIFAADPDKGTLTQLTKEG
                     EYNAEGALSPDGKRIVFTSLRDGDLDLYLMDSDGSNVRRLTTEYGYDGGPFFSWDGKY
                     IVFRSFHPQTEAERQEYAANLARHVFRPTWLELFVMNADGTGKRQVTHLGAASFAPFM
                     HPQDRQIIFASNLHDSTGRSFALYLVNVDGTGLERVTYEGGFSSFPMFSPDG"
     gene            complement(994..>1579)
                     /locus_tag="AC16G0_05955"
     CDS             complement(994..>1579)
                     /locus_tag="AC16G0_05955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020135.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR5099203.1"
                     /translation="KGRVLGGVVHILSTISAPGVIAQTAGPRTHKFGELDGRITPRTE
                     RLLQAFKVAGIQAELSSKILGDLWEKFLFICAQGGVTALGRLSIGEILACPDTAAFYR
                     GVMEEIAAVGRAKGVPLPDDAVKRALTFARSLQPHMRSSLAHDLLQGNRLEVETLSGT
                     VVRYGREVGVKTPYNFAIYACLKPSHEKAQAART"
BASE COUNT          277 a          509 c          526 g          267 t
ORIGIN      
        1 ccgtccggtg agaacattgg gaagctggaa aagccgccct cgtaggtcac tcgctcgaga
       61 ccggtcccgt ccacgttcac caggtacagg gcgaagctcc gaccggtgga atcgtgcagg
      121 ttggaggcga agatgatctg ccggtcctgg ggatgcatga agggcgcaaa actggccgcg
      181 cccaggtggg tcacctgacg cttccccgtc ccatcggcgt tcatgacgaa cagttccagc
      241 caggtcgggc gaaagacgtg gcgagccaga ttcgcagcgt actcctgccg ctccgcctcc
      301 gtctgtgggt ggaacgaccg aaagacgatg tacttcccgt cccaggagaa gaaggggccg
      361 ccgtcatacc cgtactcggt ggtgaggcgg cgcacgttgg acccgtcgct gtccatgaga
      421 tacagatcca ggtcgccatc ccggagggag gtgaacacaa tccgcttccc gtcgggagac
      481 agagcgccct ctgcgttgta ctccccttcc ttggtaagct gcgtgagagt ccccttgtcc
      541 gggtcggcgg cgaaaatatg gtacggctga agcgcccagg cgtatccgcg actcatgtcc
      601 ggccggggcg ggcactcggg gcctgccaga tgcgtcgagg agtagatgaa tcgccggccg
      661 tcggggaaga ggaaggaaca ggtcgtgacc cccttccccg tgctcaccat ccggacgtcg
      721 tttccctcca gatccatgat gtagatctgg tcgcagtgcc gtccgtcccg ggtggactgg
      781 agaatcagcc gcttcccatc gggggcgaag taggcttcgg cgttctgccc gccaaaggtg
      841 agctgacgca gattgcggaa gtgccgctcc tggggatcga ccggggcagg ccgcgccgcc
      901 caggcgcggg cgatcctgcc gagggaggcg gcgaagggga aagctgcgag gcccatcaac
      961 acgcagcgcc ggcggatcag catttggcgt tcctcacgtc cgtgccgcct gagccttctc
     1021 gtggcttggc ttcaggcagg cgtagatggc gaagttgtac ggggtcttca cgccgacctc
     1081 gcgaccgtac cgcaccaccg tccccgacag ggtctccacc tccagccgat ttccctggag
     1141 gaggtcgtgg gccagcgagg agcgcatgtg cggctgcagg ctacgcgcga aggtgagcgc
     1201 ccgcttcacc gcgtcgtcgg gaagcgggac ccccttggcc cggccgacag cagcgatctc
     1261 ctccatcacc ccgcggtaga atgcggcggt gtcggggcag gccaggattt cgccgatgct
     1321 gagccgtccc agcgccgtca cgcccccctg ggcgcagatg aagaggaatt tctcccagag
     1381 gtcccccaga atcttgcttg aaagctcggc ctgaattccg gccactttga atgcctgaag
     1441 gaggcgttcg gtccgcgggg tgatccgacc atccagctcc ccgaacttgt gggtccgagg
     1501 acccgccgtc tgggcaataa cccccggggc gctgatcgtc gagaggatgt ggaccacccc
     1561 gcccagcacg cgacccttg
//
LOCUS       DBNGZC010000331         6758 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_710588, whole genome shotgun sequence.
ACCESSION   DBNGZC010000331 DBNGZC010000000
VERSION     DBNGZC010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_710588"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="AC16G0_05960"
     CDS             complement(<1..634)
                     /locus_tag="AC16G0_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099204.1"
                     /translation="MRSPTTRLVLLLLLALTVITGVDDEIRLVRVRERLVEQTRRDQR
                     IFTETLALAVQQNLRRGRPTDGLQELIQNSLAQPDVVGIAIYNPVGEVMAERVAPGLP
                     PFVPDETVRHALAMRQAASLPVEGVSSGLRYIQPFGWPQGRTGAIEVRHSLADTEREY
                     RRAIQESILAGLMVLALFVLSGVMLTRWSIVRPIRALIAGARAVARADPAQ"
     gene            complement(681..1067)
                     /locus_tag="AC16G0_05965"
     CDS             complement(681..1067)
                     /locus_tag="AC16G0_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5099205.1"
                     /translation="MTRSPSVLVVDDDTEMRALVLEVLRNEGYEAVEAKDGAEAVQAL
                     RGRKFDLILMDKNLPGPSGLDLLPGLLRVSHGSRIVMMTAFGDLLSYVEAFEKGVAEY
                     LFKPFRIEELKAAIQKALVPDAVSRV"
     gene            complement(1560..2300)
                     /locus_tag="AC16G0_05970"
     CDS             complement(1560..2300)
                     /locus_tag="AC16G0_05970"
                     /inference="COORDINATES: protein motif:HMM:NF016269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HIR5099206.1"
                     /translation="MNNARMHPASWIKARAMAAAVLLIALTMGARPATADDAQDAHQL
                     VEKARLTFESFTADPYLGDNLRVLVNKAKAVLIYPQVLRAAFIFGASGGSGVLLARDP
                     ASGSWYGPAFYTIGEASFGLQAGADASEVVLVALTERGLSALLSTSTKLGANASIALG
                     PVGAGLTGATANISADIVSYSRNKGLYAGLSVEGAIVATRGALNAAYYGAETTPTDIL
                     IKHTVTNHDAAGLIADVTKVTGGSVATR"
     gene            complement(2458..2733)
                     /locus_tag="AC16G0_05975"
     CDS             complement(2458..2733)
                     /locus_tag="AC16G0_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099207.1"
                     /translation="MVAVTHHQPAIDQRTLIQRIRGVFARSPIFREAVISELLSGPDG
                     EAIVDVSFGQGRTAVRVVASSEDEVCEILCNLALAMDEAYPSRRAVC"
     gene            complement(3021..3365)
                     /locus_tag="AC16G0_05980"
     CDS             complement(3021..3365)
                     /locus_tag="AC16G0_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099208.1"
                     /translation="MRPLACLAAALLLVLWVASPGLARVATIETTAPLQDHAELSVKA
                     AIKEAVDAAVKGAVAMGLHWVQISKAFVLEDAVAVRILATDTAPEAGMEEAPDTNVDP
                     DVGSDQPSGFDL"
     gene            complement(3588..3932)
                     /locus_tag="AC16G0_05985"
     CDS             complement(3588..3932)
                     /locus_tag="AC16G0_05985"
                     /inference="COORDINATES: protein motif:HMM:NF018744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1344 domain-containing protein"
                     /protein_id="HIR5099209.1"
                     /translation="MRQAFTILVTLSMAGTYAWAQTMPTTPSPVGPPAQVAPSESVAP
                     QAKEVQGKVKSLDRLRKTVTLEDGTKLLIPESIKITAGSLKKGAMVIATYEERDGQKV
                     VTSILVQSPSKS"
     gene            4494..4709
                     /locus_tag="AC16G0_05990"
     CDS             4494..4709
                     /locus_tag="AC16G0_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011312550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HIR5099210.1"
                     /translation="MRRSQLRRGMIVVVLSLSTLLTAACGTLFGAAVGAGSGAAISAG
                     TGHDPGKGALIGAGVGAGVGAIYDILR"
     gene            4706..5131
                     /locus_tag="AC16G0_05995"
     CDS             4706..5131
                     /locus_tag="AC16G0_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR5099211.1"
                     /translation="MKSARTSELKSQESRRRGTPERAEALTETLHEHRRRIREELDGA
                     FRELREGMSPDTGDAGDRAVAQFDRELRSARLDQLTQMLRQVDAALSRDAAGRYGRCL
                     ACEMDIPVARLRSLPFALYCLECQAAAEEKRRGLVGSIA"
     gene            5302..5466
                     /locus_tag="AC16G0_06000"
     CDS             5302..5466
                     /locus_tag="AC16G0_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HIR5099212.1"
                     /translation="MGSLTGIGRGKPLGLRRVMALGVLVLLCLTGCHTTAGFGQDVQS
                     LGRDIQKNSK"
     gene            complement(5748..>6758)
                     /locus_tag="AC16G0_06005"
     CDS             complement(5748..>6758)
                     /locus_tag="AC16G0_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5099213.1"
                     /translation="FLDNVAGWILELDRGYGIPWKGNYSSWLAQKRERLQREQKTEGE
                     RQRTLERELEWIRMSPKGRHAKGKARISAYETLLRQEQEQLARELEIFIPPGPRLGGD
                     VIRADGVTKGYGDRALIQEMSCVVPPGAVVGIVGPNGAGKTTLFRMITGEERPDKGTL
                     VLGQTVALAYVDQARSDLDPAKTVWESISGGQDQIALGKALVNSRAYAARFNFLGPDQ
                     QKKVGALSGGERNRLHLARMLRREANVILLDEPTNDLDVNTMRALEDAIDRYAGCLLI
                     ISHDRWFLDRVATHILAFEGDSKVYWFEGNWSEYDADRKRRLGAAAERPHRIRYRSLT
                     RG"
BASE COUNT         1168 a         2235 c         2088 g         1267 t
ORIGIN      
        1 tctgggccgg gtcggctcgc gccaccgccc ttgcacccgc aatgagcgcc cggatcggcc
       61 tgacaatgct ccaccgggtc aacatcaccc cagacagaac gaagagggcc agaaccatca
      121 ggccagcaag gatgctctcc tggatggccc gacggtactc tcgctccgtg tccgccaggc
      181 tgtgccggac ttcgatggcc ccggtccgac cctgcggcca tccgaaaggt tgaatgtagc
      241 ggagaccgct ggataccccc tcgaccggga gtgaggccgc ctgccgcatt gccagcgcgt
      301 gccggaccgt ctcatccggg acgaaggggg ggagtccggg cgccaccctt tccgccatca
      361 cctccccgac gggattgtaa atggcgatcc ccacgacatc cggctgcgcc aggctgttct
      421 ggatgagctc ctgcagtccg tcggtgggcc gtccccggcg gaggttctgc tggactgcga
      481 gggcgagggt ctcggtgaag atccgctggt ctcgtcgcgt ctgctccacc agtcgctcac
      541 gcacgcggac caggcggatc tcgtcgtcca cccccgtgat caccgtcagg gccagcagca
      601 ggaggaggac caggcgggtc gtcgggctcc gcatttcccc catgacctcg acacaccagg
      661 agggcttccc gaccccccga tcacactcgg gagacggcat ccggcaccag cgccttctga
      721 atggctgcct tcaattcctc gatccggaac ggtttgaaga ggtattccgc gacacccttt
      781 tcgaaggcct cgacatagga cagcaggtcg ccaaaggccg tcatcatgac gatgcgggag
      841 ccgtggctga ccctcagaag tcccgggagc agatccaggc cactgggccc tggcaggttc
      901 ttgtccatca ggatcaggtc aaattttcgg ccccgaaggg cttgcaccgc ctcggcgcca
      961 tctttggcct cgactgcctc gtagccctcg ttacgcagaa cttcgagaac caaggcccgc
     1021 atctccgtat cgtcgtccac tacaaggacg cttggcgacc tggtcatggt cgtcgcctcc
     1081 tttcctgtgg aggagcctca tgaccgcgcg ggcaggcgca agaccaccag acagcgcccc
     1141 tcgaggtcat aagcctcgcc ctccttcacc agacgatgtg cctgccgccc gtcagccgtc
     1201 cggccctcac ggtccacctc tcacttcaac ccaggcccgt aggccgccgg caggaagaac
     1261 tgcggagctt cacggcgctc agcgacgaga tgagaagggt gatggcattg ccggccaacc
     1321 ggagggtctc gatggcggat acgtccgtgg ggctttcccc ttcacaggtt cagctcaggt
     1381 tttcgtcggt cccgtcccga ttctcctcct gattgacctc ctcgtgcttg ggcttgtagc
     1441 tcaccattcc ctcgggaaac attgttcggt ttccctggga ccatgatcct tcaatgagag
     1501 atggtccgac ccctgcaacc gatctccggc ttgagccaga ggccggcgcc agcggcggcc
     1561 taccgggtcg ccacgctccc cccggtcacc ttggtgacat cggcgatcaa cccggccgca
     1621 tcatggttcg tcaccgtgtg tttgatcaga atatctgtgg gggtcgtctc ggcgccgtag
     1681 taggcggcat tcagggcgcc tcgggtggcc acgattgccc cctcgaccga gagcccggcg
     1741 tacagcccct tgttccgcga gtagctgacg atgtctgcgc tgatgttggc cgtcgccccc
     1801 gtcaggccgg ctccgaccgg ccccagcgca atgctggcgt tggccccgag cttggtgctc
     1861 gtagacagga gggcagagag cccccgctcg gtcaatgcga cgagcaccac ctcggaagca
     1921 tcagcaccgg cctggaggcc gaagctggct tccccgatgg tatagaaggc cggcccgtac
     1981 caacttccgc tcgccgggtc gcgcgccagt agcaccccgc tgccacccga cgcgccaaag
     2041 atgaaggcgg cccgcaggac ctgagggtag atgagcaccg ccttggcctt gttgacgagc
     2101 actcgcaggt tgtcacccag atacggatcc gcggtgaagc tctcgaaggt gagacgcgcc
     2161 ttctcgacga gctggtgcgc gtcctgggcg tcgtcggccg tcgcgggcct cgcacccatg
     2221 gtcagagcaa tcagcagcac cgccgccgcc atggccctcg ccttgatcca gctcgccgga
     2281 tgcattcttg cgttgttcat cggtcactcc tttcacgtgt gcaatccgtc tgtgcggggt
     2341 tctccacctc ccagtccaag ccaaggacct tcgacccacc cctcctttct gcgtttcggt
     2401 tttcggcggg tccactctcc ccgcctgtca agcttcccgt cctatgccac cgcgttctca
     2461 gcacacggca cgacggcttg gataggcttc gtccattgca agggccagat tacagaggat
     2521 ctcacacacc tcatcttcgg aggatgcaac caccctgaca gccgtgcgac cttggccaaa
     2581 actgacgtcc acgatagctt cgccgtcagg accggagagc aattcggaaa tgacggcctc
     2641 gcggaagatc ggagaccgag caaagacccc tcgtatccgt tgtatcagcg tgcgctgatc
     2701 gatcgccggt tgatgatgcg ttacagccac catgtgtcac ctcctgcaat gctcttcgct
     2761 ggggatctaa ggtctggctg gtccggcagg ggtggaagag ttgctcttcc agcccgcctg
     2821 ccgcaccttg actgatctcc ccgcaacggc gaggaaggtg tcatctcgtc gatcgagagc
     2881 gaggacgacg tgcccagggt gaagaaggca cacccggaac gaccataggg gcatcgtgga
     2941 tctccggctg aagcccgagg atcaggcccg atcccaagga gaagcggagg tccagcctgg
     3001 gccgatggtt cgcatggacc tcacaggtca aatcccgaag gttgatcgga tccgacatcg
     3061 ggatcgacgt tcgtatccgg cgcctcttcc atccctgcct cgggagccgt gtcggtcgca
     3121 aggatccgga ccgccacggc gtcctcaagt acgaaggcct tgctgatctg aacccagtgg
     3181 agacccatcg caaccgcgcc cttcacggcg gcatccaccg cctccttgat ggccgccttg
     3241 acggacagct ccgcgtggtc ctggaggggt gcggtggtct cgatggttgc caccctggcc
     3301 aaacccggcg acgcgaccca aaggaccagc agaagcgccg cagcgaggca agcgagcgga
     3361 cgcatgatcc acctcctcgg ggggatcggg ggcggcagtg cccgggtcga tagccggcag
     3421 gcacttcgcc tgaatcccgt cccccgtatg atcggctctc gaggccgatc ccggtttaac
     3481 tcttgggggg ggaacgttgc tcgtactttc ctgctcgtgg cagccgcgtg ggaaggatcg
     3541 aaggcccgga aacctcctgc cgggcctgcg gtgtgcgagc aggcgtgcta ggacttcgac
     3601 ggcgactgca ccaggatcga tgtcaccact ttctgcccat ctctctcttc gtaggtcgcg
     3661 atgaccatcg cccccttctt caaggacccc gccgtgatct tgatggactc ggggatcaga
     3721 agcttggtac cgtcctcgag agtcacggtc ttcctcaacc gatcgaggct cttgaccttc
     3781 ccctggacct ccttggcttg gggcgccaca gattccgatg gagccacctg ggccggggga
     3841 ccaaccgggc tcggggtggt tggcatcgtc tgggcccacg cataggttcc ggccatggag
     3901 agggtgacca ggatcgtgaa agcctgcctc attcttgcga cctcctagga gcacccgtcg
     3961 taccctgtat cccctgctcc ggaccatcca gactcccagg gaaagcgaca gcgaagctcc
     4021 gcattctgca taggcaaacc gagtgccagt cccgtgaaac ctgattggat catggttgat
     4081 aatctttatt tttcaataac ttaccaataa caatgtggcc ggacagtagt cttgccgggg
     4141 attgttggaa gatggttcaa ccggctggat ggaggagaat agagagtaaa ttttccctcc
     4201 cacagccttt gcgggccggt tccgtgggac ttttctggaa aaatgcgcag ttccaaggtc
     4261 acggagccga gtaataccta tagaagccat catataacta tatgatttat aagatgcatg
     4321 taattattac agagttagtc ttgtatgaag ccaacctgag cattcccgcc ggcaaccatg
     4381 aagctctttg taagtcacca actttgcagc gccattctct atgtggctag ttttcaattt
     4441 tgccttggca tccgccatgc tttggggtcc gggtggaagg aaggaggccc tacatgcgca
     4501 gatctcaatt gcgtcgaggg atgatcgtcg tggtcttgtc cctctccacg ctgctaacgg
     4561 cagcctgcgg cactctcttc ggggcagcgg taggcgctgg cagcggagcc gccatcagcg
     4621 ctggcaccgg ccacgacccc ggtaaggggg ctctcatcgg cgccggtgtt ggggccgggg
     4681 tcggcgccat ttacgacata ctcagatgaa gagcgcgcgc acctccgagc tgaagtctca
     4741 agaaagccgg cggcggggca ccccggagcg cgccgaggcc ctgaccgaga ccctgcacga
     4801 acatcgacga cggattcggg aggaactgga cggagccttc cgcgaactcc gggagggaat
     4861 gtcacccgat acgggggatg cgggggaccg ggcggttgct cagttcgatc gcgaactccg
     4921 ctccgcgcgg ttggatcaac tcacccagat gctacggcag gttgatgcgg cgctctcccg
     4981 agatgccgcg ggccggtacg gccggtgtct cgcctgcgag atggacatcc cggtggcgcg
     5041 gctgcgttct ctccccttcg cgctctattg cctcgagtgc caggcagcgg ctgaggagaa
     5101 gcgccgcggg cttgttggtt caatcgcgta agccgcacgg atggacggct gggagggtgg
     5161 cagtgtcaga gtcggggcgc ttctcggcgg caaggcttcg accgtcgacg gccacatgcc
     5221 cgccacgagg gtccggtcgg tcgatgcctc tctccgagca actgagtggg cttggcccag
     5281 tcaacatagg gaggagaacc catgggaagt ctaacaggga tcggtcgggg gaaaccgctc
     5341 ggcctgcggc gcgtcatggc cttgggggtg ctcgtcctgc tctgcctgac ggggtgtcac
     5401 accactgccg gcttcgggca ggacgtccag tcgctcggcc gcgatattca gaagaactcg
     5461 aaatagcgcc ctggtctcga ggacgctgac gacactcggc ggagcgcaag gaaccaaccg
     5521 cggttcgcct cgggtgtcaa ggaagggcgc gaccccaaat cggtccctcc cggggtcgcg
     5581 cccgctttct ttccaattgg tcccccgctg acctccggac agcccacgga accgagtcag
     5641 gcggtccacg cgtgttgccc agtgcggccg gctaatcgac gacgctgggg cggcagccgc
     5701 atgacagact gccacctgac cgatggtccc tccctgatgg agcctcctca tccccgcgtg
     5761 agcgaccggt agcggatccg atgcgggcgc tcggccgcgg cgccgaggcg gcgcttccgg
     5821 tcggcgtcgt actcgctcca gttcccctcg aaccagtaca ccttgctatc gccctcgaat
     5881 gcgaggatgt gggtcgcgac gcggtcgaga aaccagcgat cgtgggagat gatgaggagg
     5941 cacccagcgt agcggtcgat ggcgtcctcg agcgcgcgca tggtgttgac gtcgaggtcg
     6001 ttcgtcggct cgtcaagcag gatgacgttc gcctcgcggc ggagcatccg cgcaaggtgc
     6061 aaccggtttc gctcgccccc cgagagcgcc ccgaccttct tttgctggtc gggaccgagg
     6121 aagttgaagc gcgccgcgta ggcgcgcgag ttgaccaggg ccttcccgag cgcgatctgg
     6181 tcctgcccgc ccgagatcga ctcccacacg gtcttcgccg ggtcgaggtc gctgcgggcc
     6241 tgatcgacgt aagccagcgc caccgtctgg cccagaacca gcgtcccctt gtcgggtctt
     6301 tcctcgccgg tgatcatccg aaacagcgtg gtcttccccg ccccgttcgg cccgacgatc
     6361 cccacgaccg ctcctggcgg gacgacgcag gacatctctt ggatgagcgc ccggtcgcca
     6421 taacccttgg taaccccgtc ggcccggatg acatcgcccc cgagccgcgg cccgggcggg
     6481 atgaagatct caagctcgcg cgcgagctgc tcctgttcct gccggaggag cgtctcgtag
     6541 gcgctgatgc gcgccttccc cttcgcgtgt cggcccttcg gcgacatccg gatccattcg
     6601 agttcgcgct cgagggtgcg ctgccgctcg ccctcggtct tctgctcccg ctggagccgc
     6661 tcccgtttct gcgccagcca cgatgagtag ttccccttcc acgggatacc gtatccgcgg
     6721 tcgagctcca ggatccagcc tgccacgttg tcgaggaa
//
LOCUS       DBNGZC010000332         6218 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1689957, whole genome shotgun sequence.
ACCESSION   DBNGZC010000332 DBNGZC010000000
VERSION     DBNGZC010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6218)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6218)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6218
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1689957"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="AC16G0_06010"
     CDS             complement(<1..848)
                     /locus_tag="AC16G0_06010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5099214.1"
                     /translation="MDPIALLTRMVEIPSLSGEESHLASFLVETMRALGFQASVDQAG
                     NAVGILGDGPTEIVLLGHMDTVPGALSVRVENGKLYGRGSVDAKGPLAAFIAAAAQVG
                     ALPGKRIVVVGATEEEAASSKGARYATEVFAPHFCVIGEPSGAYAVTLGYKGRIVCAY
                     DASTGATHSASKEGSILEEGVEFWNQIRRWADEFNVGKKMFDRLDPALREIRSENDGL
                     TESIRLRLSVRIPQGLSPEVVRRHVEALSSDPARLRFWGADPPYRAEKNTPLVRAFLN
                     AIRQQGT"
     gene            complement(914..2092)
                     /locus_tag="AC16G0_06015"
     CDS             complement(914..2092)
                     /locus_tag="AC16G0_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR5099215.1"
                     /translation="MDFKTIEDQSSVGVYQKRDLVLVRGKGARVWDAAGREYIDCVAG
                     VGVANVGHCNPAVVSAIHDQAARLITCNELFYNDARAHCLERLNKITPEGIDRFFLCN
                     SGTEAVEGAIKFARMATGRKRVVAAMRGYHGKTLGSLSATWDKKYRDPFAPLVPDFAH
                     VPYNNVAEFEKAIDDQTAAVILEPVQGEGGVRPATAEFFQAVRKLCDDRGVLFIIDEI
                     QTGFGRTGRMFACEHFGVLPDILTMAKGIAGGIPMGAIGIDRRVGDIEKQSHTSTFGG
                     NPLACAAAVAAIDVLVEQDLPRRAAESGAYFFQRLREIGSPRIREIRGLGLMIGLELK
                     EKAGPLAQALMREGILVLLAGTTVLRFLPPLVITQAEIDSVITALTHVLADSPGATAE
                     "
     gene            complement(2206..3009)
                     /locus_tag="AC16G0_06020"
     CDS             complement(2206..3009)
                     /locus_tag="AC16G0_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-aminoadipate kinase"
                     /protein_id="HIR5099216.1"
                     /translation="MLVVKIGGGSGTQIEPAVADLTEILRAGRKVILVHGASGETNVL
                     AEKLGKPPRFVTSVSGIESRYTDRETLEIFEMAYCGKANKAVVEALQRRGINAVGLSG
                     LDGRIFEGKRKDTLTVFESGKRKVLRGDYTGKVERVNAALVTLLLDHGYLPVLCPPAS
                     SYEGEAVNVDGDTASAMLAAAMKAEEFILLSNVPGLLRDLADPKSLVARIPAKAIEEF
                     MPLAHGRMKRKLMAASIALGAGVGRVILASANVEHPIVSALAGRGTVIG"
     gene            complement(3053..4093)
                     /gene="argC"
                     /locus_tag="AC16G0_06025"
     CDS             complement(3053..4093)
                     /gene="argC"
                     /locus_tag="AC16G0_06025"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR5099217.1"
                     /translation="MISVGIVGASGYTGGEALRILLRHPEARVIQATSESNAGHFVYA
                     IHPNLRKQCQLKFTALGDLKPCDVLFLCLPHGNAMRHIASLEGVAKTIIDLSADFRLR
                     NPDDYPVWYGHPHAAPEYLGRFVYGIPELHRKAISQARFITGAGCLATTSILGLLPLF
                     RHDLLSRREVVIEAKVGSSAAGDTPHLSTHHPERSGSLRSFEPTGHRHTAEIVKELGF
                     GQPLAVHFSATAIEAVRGVLATCHVFVKDGVEERDLWKAYRAEYGDEPFLRIVKERQG
                     IYRYPEPKLLAGSNFCDVGFEKDARGSRVVVMAALDNLVKGAAGNGVQAMNVACALPE
                     TAGLDFPGLHPV"
     gene            complement(4164..5009)
                     /gene="lysX"
                     /locus_tag="AC16G0_06030"
     CDS             complement(4164..5009)
                     /gene="lysX"
                     /locus_tag="AC16G0_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006926898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HIR5099218.1"
                     /translation="MRIGVLCSLVRKEEKLLFDEFRRQDLPFDRIDDRELILDLHHRQ
                     WDFDVILERCINHSRALHTLKYLNDCGVKTVNTYEVASTCGDKLLTTLALIREGVPTP
                     RVFVAHTPESALQAIEQLGYPVVLKPAVGSWGRLLSKVNDRDAAEAILEHKQTLGSYH
                     HSIFYIQEYVEKRGRDIRSFVVGDETICAIYRTSPHWITNTARGGVATNCPVTPEVNE
                     LSVRAAKAVGGGVVAIDLFETPDGITVNEVNYTMEFRNSIDTTGVNIPAKVVDYVREV
                     ARRRA"
     gene            complement(5006..5170)
                     /gene="lysW"
                     /locus_tag="AC16G0_06035"
     CDS             complement(5006..5170)
                     /gene="lysW"
                     /locus_tag="AC16G0_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysW"
                     /protein_id="HIR5099219.1"
                     /translation="MATCTECEAEVPLEASTEVGEIIQCPDCGLDLEVISLEPPALAP
                     APEDEEDWGE"
     gene            5439..6179
                     /locus_tag="AC16G0_06040"
     CDS             5439..6179
                     /locus_tag="AC16G0_06040"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR5099220.1"
                     /translation="MLRAVTFDLWQTLLLDTPEGLHRARADRIRGLQAVLARHGHWVQ
                     HDRIGQAYDAVGTRLEEIWETRKDLGSRGQVHLLLEDLGLDQQVPREGPLMDALDDAY
                     CLPILSALPTVNDGAREILKSLEHRGLRLALICNTGRTSGRMLRLVLDRLALAPYLRV
                     LTFSDEVGLRKPHPEIFWRTLSALGVAPESAAHVGDDVTTDVAGARGVGMRAVHLQHG
                     TSVTQGSDGTEVIPSLHALPAVLFHDLS"
BASE COUNT         1063 a         2008 c         2062 g         1085 t
ORIGIN      
        1 gtcccctgct ggcgaatggc attgaggaag gcccggacca gcggggtgtt cttctcggca
       61 cggtaggggg ggtcggctcc ccagaagcgg aggcgcgccg gatcagacga gagggcctcc
      121 acgtgccggc gcacgacctc cggagaaaga ccctggggga tccggaccga cagccgcagc
      181 cggatggatt ccgtcagtcc atcgttttcg ctccggatct cgcggagcgc cggatccaga
      241 cggtcgaaca tcttcttgcc cacgttgaat tcatccgccc agcgtctgat ttgattccag
      301 aactccactc cttcctccag aatgctgcct tccttcgagg cggagtgcgt tgcgccggtg
      361 ctcgcatcgt aggcacagac gatccgtccc ttgtacccta acgtgacggc ataggcacca
      421 ctgggctcgc cgatcacgca gaagtgtggc gcgaagacct cggtcgcgta ccttgcccct
      481 ttggagctgg cggcttcctc ctcggtcgcg cccaccacga caatccgctt gcccggaagg
      541 gcacccacct gggccgccgc ggcgatgaag gctgccagtg ggcccttggc gtccacgctc
      601 ccgcgaccgt agagcttgcc gttttcgacg cgcaccgaca gggcacctgg cacggtgtcc
      661 atgtggccga ggaggacgat ctcggtcggg ccgtcgccca ggatgccgac ggcattcccg
      721 gcctggtcca ccgaggcttg gaagccgaga gcgcgcatgg tctcgacgag gaaggaggca
      781 agatgagact cctcaccgga gagggagggg atctcgacca tgcgtgtgag cagggcgatg
      841 gggtccatgg atcaagcgga ggcgagccga gagccgcgag ccgagagccg ccatctcctc
      901 gcgcattcat gcatcattcc gcggtggctc cgggcgagtc ggcgagaaca tgggtgaggg
      961 ccgtgatcac gctgtcgatt tctgcttggg tgatgacgag gggcggaagg aaccggagga
     1021 cggttgtgcc ggcgaggagg accaggatgc cttcccgcat cagggcctgg gcaaggggac
     1081 cggccttttc cttcagctcc aggccgatca tcaggcccag gccgcggatc tcgcggatgc
     1141 gggggctgcc gatctcccga aggcgctgga agaaatacgc cccgctctcc gccgcgcgcc
     1201 gggggaggtc ctgctccacc agcacgtcga tggcggccac ggctgcggcg caggccaagg
     1261 ggtttccgcc gaaggtggag gtgtgggact gtttctcgat gtcgcccacg cggcggtcga
     1321 tcccgatggc gcccatggga atcccgccgg cgatcccttt cgccatggtg aggatgtccg
     1381 gcagcacccc gaagtgctca caggcaaaca tgcggccggt ccgtccgaag cccgtctgga
     1441 tctcgtcgat gatgaagagg acaccccggt cgtcgcacag cttccggacc gcctggaaga
     1501 actcggccgt ggccggccgg acgccgccct cgccctggac cggctccagg atcacggcgg
     1561 cggtctggtc gtcgatggcc ttctcgaact ccgccacgtt gttgtagggc acatgggcga
     1621 agtccgggac cagcggggca aaggggtcgc ggtacttctt gtcccaggtg gcgctcagcg
     1681 aacctagagt cttgccgtga tacccgcgca tggcggccac cacgcgcttc cgcccggtgg
     1741 ccatgcgggc aaacttgatg gcaccctcga ccgcctcggt gccggagttg cagagaaaaa
     1801 agcggtcgat cccctcgggc gtaatcttgt tgagtcgctc caggcagtgc gcccgggcgt
     1861 cgttgtagaa cagctcgttg caggtgatca gacgggccgc ctgatcgtgg atggccgaca
     1921 cgaccgcggg gttgcagtgg cccacgttgg ccacgccgac gccggcaacg cagtcgatgt
     1981 actcccgacc ggccgcgtcc cacacgcggg cgcccttccc gcgcacaagg accaggtccc
     2041 gtttttgata caccccgacg gaggactgat cttcgatggt cttgaaatcc atgctctcaa
     2101 tcccctgtcc tgggctgccg ggcacagcgc agagtgcatg gggcccaggg tctgccgctc
     2161 tattcagttc tcaatgccct atgcgctttg ccctgtgcgc tgtgcctacc cgatcacggt
     2221 cccccggccc gcgagggcgg agacgatggg atgctccacg ttggcactgg ccaggatgac
     2281 gcgcccaacg cccgccccca gggcaatgga ggcggccatg agcttccgct tcatccggcc
     2341 gtgggccaac ggcatgaact cctcgatggc cttggccggg atccgcgcca ccaaggactt
     2401 cggatcggcg agatcgcgga gaaggcccgg cacgttcgag agcaggatga actcctccgc
     2461 cttcatcgca gccgcgagca tggccgacgc cgtatccccg tccacgttga cggcctcccc
     2521 ctcgtaactg ctggccggcg ggcagagaac gggcagatag ccgtgatcca ggagcagggt
     2581 cacgagggcg gcgttgaccc gctcgacctt tcccgtgtag tcgccgcgca agaccttccg
     2641 cttcccactc tcgaagaccg tgagcgtgtc cttgcgcttc ccctcaaaga tccgcccgtc
     2701 cagaccggag agtcccacgg cgttgattcc acgtcgctgg agcgcctcca ccacggcctt
     2761 gttggccttg ccgcagtagg ccatctcgaa gatttccagg gtctcccggt cggtgtaccg
     2821 gctctcgatt cccgacacgg aggtcacgaa gcgcggcggc ttccccaact tctcggccag
     2881 gacgttggtc tcgcccgagg cgccatggac gaggatgacc ttccggcccg cgcggaggat
     2941 ctccgtgaga tccgccaccg cgggctcaat ctgtgtcccc gatccgccac caatcttgac
     3001 gacgagcatc gcctctccga ggtgcaaggg ccgagggaag ccctggaacg tgctataccg
     3061 ggtgcaatcc ggggaagtcc aagccggccg tttcggggag ggcgcaggcg acgttcatgg
     3121 cctggacgcc gttcccggcc gcgcccttca ccaggttgtc cagggcggcc atgacgacca
     3181 cgcgagaccc gcgggcatcc ttctcgaagc cgacgtcgca gaaattgctg ccggccagga
     3241 gtttgggctc ggggtagcga tagatgccct gccgctcctt gacgatccgc aggaaaggct
     3301 cgtctccgta ctcggcccgg taggccttcc agagatcccg ctcctcgacc ccatccttga
     3361 cgaagacgtg gcaggtggcc aggacgccgc ggacggcctc gatggcggtg gcgctgaagt
     3421 gaaccgccag cggctgtccg aagccaagct ctttgacgat ctcggcggtg tgccggtgac
     3481 cggtcggctc gaaagagcgg agcgagccgc tgcgctcggg gtggtgcgtg gagaggtggg
     3541 gcgtgtcccc agccgcggag gaccccacct tggcctcgat cacgacctcg cgccgcgaca
     3601 ggaggtcgtg cctgaagagc ggcaagaggc ccaggatgga ggtcgtggcg agacaaccgg
     3661 cacccgtgat gaatcgagcc tggctgatgg ccttgcggtg cagctccggg atcccgtaga
     3721 caaagcggcc caggtactcc ggggccgcgt ggggatggcc gtaccagacg gggtagtcgt
     3781 cgggatttcg gagccggaag tcggcgctga ggtcaatgat cgtcttggcc acaccctcca
     3841 gcgacgcgat gtgtcgcatg gcgttgccgt gggggaggca gaggaatagg acgtcgcagg
     3901 gcttcaggtc acccagggcg gtgaacttga gctggcattg ctttcgcaga ttgggatgaa
     3961 tggcatagac aaagtgcccc gcgttggatt cggacgtggc ctggatgacc cgggcctccg
     4021 gatgacggag caggatgcgg agagcttcgc cgcctgtgta gccggaggcg ccgacgatgc
     4081 caacgctgat catgaggaac tccccaaaga tgggaattgt gttcggtcgt tcgggggtcc
     4141 gatggttcgg ctggtcggtg gtctcacgct cggcgccggg caacttcccg gacgtagtcc
     4201 accaccttcg ccgggatgtt cacacccgtg gtgtcgatgc tgttccgaaa ctccatggtg
     4261 taattgacct cattcacggt gatgccgtcc ggcgtctcga agaggtcaat ggccaccacc
     4321 cctccgccca cggccttggc tgcccggacg gagagttcgt tcacctccgg tgtcacgggg
     4381 caattggtgg ccacgccgcc ccgcgcggta ttggtgatcc aatggggcga ggtgcggtag
     4441 atggcgcaaa tggtctcgtc ccccacgacg aaggagcgga tgtcccggcc gcgcttctcc
     4501 acgtactcct gaatgtagaa gatggagtga tggtaggagc ccagcgtttg cttgtgctcc
     4561 aggatggcct cggccgcgtc acgatcgttc accttggaga ggagacgccc ccaggacccc
     4621 acggccggct tgagcaccac cgggtatccg agctgctcga tggcctgcag cgccgactcg
     4681 ggtgtgtggg ccacgaacac ccggggagtc ggtacccctt cccggatgag ggccagagtc
     4741 gtgaggagct tgtcgccgca ggtgctggcg acctcgtagg tgttgaccgt cttgacgccg
     4801 caatcgttca ggtacttcaa agtgtgcagg gctcgggagt ggttgatgca gcgctccaag
     4861 atgacatcga aatcccactg ccggtgatgg agatcgagga tcaactcccg atcatcgatc
     4921 cgatcgaacg gcaggtcctg ccggcggaac tcatcgaaca ggagcttctc ctctttgcgg
     4981 accagggagc acaagacgcc gattctcatt cgccccaatc ctcctcgtcc tcaggcgccg
     5041 gggccagtgc cgggggctcc aggctgatca cctccagatc cagtccgcag tcggggcatt
     5101 ggatgatctc gcccacctcg gtggacgcct ccagcgggac ctcagcttcg cattcggtgc
     5161 aggtcgccat ggtcgtcacc tccaaggaaa gtacatataa tgggtcggga ggatagcacg
     5221 cacgtcccgc cccttcaagg gaaacgcgac cacccagcca ataatgtacg gcgctgatag
     5281 cgaatcgggc cgattgtcag cgcgggactc ctgtgctact ttccaggcta gaggcaagag
     5341 aacgtacggt aggccgactt tggcgatcag ccatcaactg ctgatcgccg ttgtcaatca
     5401 tcgaaagccg atggtaaggg ctttcacgca aggagaccat gctgagagcg gtgacctttg
     5461 atctgtggca gacgctgctc ctggacacac ccgagggact gcaccgcgcc cgcgcggatc
     5521 gaatacgcgg gctccaggcg gtcttggcac ggcacggcca ttgggttcag cacgatcgca
     5581 tcggtcaggc ctacgacgcc gtggggacca ggctggagga aatctgggag acccggaaag
     5641 atctggggtc tcgcggacag gtgcatctgc tcctggagga tctcggattg gaccagcagg
     5701 tcccgcggga gggcccgctc atggacgccc tggatgacgc ctactgcctc cccatcctgt
     5761 cggcgctgcc cacggtcaac gatggagccc gggagatcct gaagtccctg gaacaccgag
     5821 gcctacgcct ggcattgatc tgcaataccg gacggacgtc gggcaggatg ctgcgcctgg
     5881 tcctggatcg tctggcgctg gcgccatacc ttcgggtcct caccttctcc gatgaggtcg
     5941 gtctgcgcaa gccgcacccg gagatcttct ggcgaaccct ctcggctctt ggggttgccc
     6001 ctgaatccgc cgcgcacgtc ggcgatgacg tcaccaccga tgtggccggg gcgcgcggcg
     6061 tcggcatgcg ggccgtccac ctgcagcacg ggacgagcgt gactcagggc tcggacggca
     6121 cggaagtgat ccccagtctg cacgcccttc ccgccgtcct gttccacgat ctgtcctgat
     6181 cccacgagcg cggccgcagg ctacgcagac cgacctcg
//
LOCUS       DBNGZC010000333         3640 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_322226, whole genome shotgun sequence.
ACCESSION   DBNGZC010000333 DBNGZC010000000
VERSION     DBNGZC010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3640)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3640)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3640
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_322226"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="AC16G0_06045"
     CDS             <1..726
                     /locus_tag="AC16G0_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HIR5099221.1"
                     /translation="HLRRAKAAVAIPVIGSLNGVSTGGWIRFAKQIEETGVDGLELNV
                     YYIPTDPEMSGARVEQMYLDLVRDMRASVKIPVAVKLGPYFSAMAHMARRLDQVGADA
                     LVLFNRFYQPDFDLDKLEVVPNLRLSTSDELRLRLCWAAILYGHVRCDLAITGGIHTA
                     ADVVKAMMAGARAAMTTSALLMHGVEHLTTLRAGLVAWMEEHEYESVRQMQGSMSQKS
                     VAEPAAFERANYMKVLRSYALRK"
     gene            835..1491
                     /locus_tag="AC16G0_06050"
     CDS             835..1491
                     /locus_tag="AC16G0_06050"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR5099222.1"
                     /translation="MEIGGEEKGLSTMPRLGAQAPAFQAETTHGTIRLEDFRGSWLIL
                     FSHPADFTPVCTTEFIAFAEIAPELRQRNVELLGLSIDSTYSHIAWVRNIEQHFGVKI
                     PFPVIADLNKEVASLYGMIMPGESKTETSRCVFVIDPNGLLRAMIYYPLTTGRNMDEI
                     LRLIDALQVTDKHGVATPANWRPGQKVIVPPPKTADGAEERMQAGFECTDWYFCKKTL
                     "
     gene            1504..1731
                     /locus_tag="AC16G0_06055"
     CDS             1504..1731
                     /locus_tag="AC16G0_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099223.1"
                     /translation="MGCINPDGTTDPAARTLLQALQTPRNPEELAAAAGLPLYRIRSG
                     LRDLGQAGLVEEASGKFVLTAAGRARVGSSV"
     gene            2823..>3640
                     /locus_tag="AC16G0_06060"
     CDS             2823..>3640
                     /locus_tag="AC16G0_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009771425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HIR5099224.1"
                     /translation="MYHQNYNPLGNVFVSTLVAAIPILTLLYFIALHPHRDAKGGKHL
                     GISAPYAAFYGVLAAFLVSCLAFGMPFPSAFSAFVIGSLSGFLGIIWIIIGAIFLYNM
                     TLITGKFEIVKESIIHISFDRRLQVLLIAFSFGAIIEGTSGFGTPVAIAGAVMVGLGF
                     SPFQSAVLNLLANTAPVAWGAIGTPIVTLAAVSGLDQLTLSHMAGRQLPFVSVLVPFW
                     LVTTFVLMEGGTWKEAWEVWPGTLCSGLSFAVMQWFASSTNEFHLMTDVVSGVFS"
BASE COUNT          617 a         1138 c         1164 g          721 t
ORIGIN      
        1 cacctgcgcc gggcgaaggc ggcggtggcc atcccggtca tcggcagtct caacggggtc
       61 tccaccggcg gctggattcg ctttgccaag cagatcgagg agacgggggt cgacggactg
      121 gagctgaatg tctactacat ccccaccgat cctgagatgt ccggtgcccg ggtcgagcag
      181 atgtacctcg acctggttcg cgacatgcgg gccagcgtca agatcccggt ggccgtcaag
      241 ctcggcccct atttcagcgc catggcccac atggcacgcc gtctggatca ggtcggggcc
      301 gacgccctgg tgctcttcaa tcgcttctac cagcccgact tcgatctcga caagctcgag
      361 gtcgtgccca atctacgctt gagcacatcc gacgagctgc gccttcgcct gtgttgggcg
      421 gccattctct acgggcacgt ccggtgcgac ctggcgataa cgggtggtat tcacaccgcg
      481 gcagacgtgg tgaaggccat gatggcgggc gccagggccg ccatgacgac gtccgccttg
      541 ctgatgcacg gcgtggagca cctgacgacc ctccgggccg gcttggtggc ctggatggaa
      601 gagcatgaat acgagtccgt ccgccagatg cagggcagca tgagtcaaaa gtcggtggcc
      661 gagcctgctg ccttcgaacg ggccaactac atgaaggtgc tgcgctcgta cgctttgcga
      721 aagtaaacag gatacgctga ggggctccgc cgccttgctc attttcgatg gggcggccag
      781 tcaattccca gcgggacccc gcggccagcg aagggtggaa aggggggatc gttcatggag
      841 atcggcggag aggaaaaggg cctgtcaaca atgccgcgct tgggggcgca ggcgccggcc
      901 ttccaggccg agaccacgca cggaacgatt cgtctggaag acttccgggg gagttggctg
      961 atcctcttct cccacccggc agacttcacg ccggtgtgca ctaccgaatt cattgccttt
     1021 gccgaaatcg cacccgaact ccggcagcga aacgtggaat tgttgggcct cagcatcgac
     1081 agcacctact cccacattgc ctgggtgcgc aacatcgagc agcacttcgg tgtcaagatt
     1141 cccttcccgg tcatcgccga cctgaacaag gaagttgcct cgctctacgg catgatcatg
     1201 cccggtgaga gcaagacgga gaccagccgg tgcgtctttg tgatcgaccc caacggcctc
     1261 ttgcgggcga tgatctacta tcccctgacc accggccgca acatggacga gatcctgcgc
     1321 ctgatcgacg ccctgcaggt caccgacaag cacggggtgg ctacgccggc caactggcgc
     1381 cctggccaga aagtcatcgt cccgccgccc aagaccgctg acggtgccga ggagcggatg
     1441 caggctggct tcgagtgcac ggactggtat ttctgcaaga agacgctctg agggagagat
     1501 cgcatgggct gcatcaatcc cgacgggacg acggatcccg ccgctcggac gctgctccag
     1561 gcgctgcaga cgccgcgaaa tccggaggag cttgcagccg cggctggcct tccgttgtac
     1621 cgcatccgga gcggcctccg cgatctcggc caggccgggc tggtagaaga ggcgagcggg
     1681 aagtttgtcc tcaccgccgc cggccgggca cgcgtcggct cgtccgtgtg acctgcggcc
     1741 ggatacgccg gacctcggtg cgatgcgtta gattccaagg acaccccgtc gtgaccccgt
     1801 gtgggatggg atcgcggcgg ggtgcctgta tttggtacac tggttttctg gctccgtccc
     1861 ccgaacgtcg tcccccctcc tgtgtctgtt gccgattgcc catcttctgg tgtgctcgga
     1921 ttggtgtgaa gaaacgggcg ggatctcctt gcttggctcg aattgtctcc ggtttggtcg
     1981 gacaagtttc cagacgaggc gggaacgggg gcttgacatt tcacatagca tgggactagc
     2041 atgcgccggc ttcgttggtg atgggctcgc gaggcccgcg agcaggacgc ggggaatctt
     2101 gggcgaacag tgactacggg catggccggg atggcagacg gggctgccat ctgggtccgt
     2161 ggccgaaact cccaacaccc ccgaccaacg agcaggattc gaggacgtcg gcccggttgg
     2221 gcaccacatc cagccagaat ttctgatcgg caacctctcc ggagggctga tccgtggcgt
     2281 ggggatgttc ctcctcggga tctgaaccgg cgcctgtccg gaagaccttg attgagaaag
     2341 gtccaggcga cgggtcttct ccgaaggttc tggagagcct cggccgggcg atcctctcct
     2401 atccggttca ttccttctca ccgaatccac tcattccagt ggcacaggtc aggaagaccg
     2461 gcctgccctc tcggcaggtc gctatcgacc ccccgtggcc ggcgcgagcc ttctctcagc
     2521 gcaggtgtcg tctttccacc cgagtcacgc cagttgttct cgggtattcg agggggtcgt
     2581 ggacttcgcc gcggccggtg agggtgctgc ggcatacgga tgataagggg aacgggcagt
     2641 ccccggtccg atggggaagg aggtggtcgt ctcagagact cgaaggccgc gggtgagatg
     2701 aggcccgcga tgccggggtg cctcagggca agcgtgagtg gaaaatcgct gatgcgcctc
     2761 gggaggtgaa gctggttacg gtcggggatt acatcccgaa ccgaatcctc ggaggaagac
     2821 cgatgtacca tcagaactac aacccattgg gtaatgtctt tgtctccacc cttgtggcag
     2881 cgattccaat ccttacgttg ctgtacttca tcgccttgca cccgcaccgt gacgcgaaag
     2941 gcgggaagca tctcggcatc agcgccccgt atgcggcgtt ctatggggtc ctggcggcgt
     3001 tcctcgtctc ctgcctggcc ttcgggatgc cgtttccctc ggcgttttca gccttcgtta
     3061 tcggcagcct gtcagggttc ctggggatta tctggatcat catcggggcc atattcctct
     3121 acaatatgac cctcatcacc gggaagttcg agatcgtgaa ggagtcgatc attcacatct
     3181 cgttcgaccg gcgcctccag gtcctcctga tcgctttctc cttcggggcc atcatcgagg
     3241 ggacctccgg gttcgggacg ccggtggcca tcgccggggc agtcatggtc ggcctcggct
     3301 tctccccctt ccagtcggcc gtcctgaatc tgctggccaa caccgccccc gtggcgtggg
     3361 gggccatcgg cacgcctatc gtgaccctcg cagccgtgag cggcctcgac cagctcaccc
     3421 tttcccacat ggccggacgc cagctcccct ttgtgtccgt cctggttccc ttctggctgg
     3481 tcaccacctt cgtcttgatg gaggggggca cctggaagga agcctgggaa gtctggccgg
     3541 ggacgctgtg ctcgggccta tcctttgccg tcatgcagtg gttcgcctcc tcgacgaatg
     3601 agttccacct gatgacggac gtggtctcgg gcgtcttctc
//
LOCUS       DBNGZC010000334         3780 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2667837, whole genome shotgun sequence.
ACCESSION   DBNGZC010000334 DBNGZC010000000
VERSION     DBNGZC010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2667837"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..>3780
                     /locus_tag="AC16G0_06065"
     CDS             55..>3780
                     /locus_tag="AC16G0_06065"
                     /inference="COORDINATES: protein motif:HMM:NF024627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR5099225.1"
                     /translation="MELAWVRTPLKDGPTNLYARIWNGTHFVEQIPGEAQEPGVSVSG
                     DEAQGLSLALDASGRPIVAWQEAQEGSPEIFVRATQFDLGTIYTADGTPGHTLQETLD
                     SHDLGAGDVIVVAGTQAGFTVSGDDAGVLIAGAPGAVITGNVIIAAAATSVTLQRLLI
                     QGSLDLAADGVTVTESEVRGGTSLTGGSGQAIVYSRLAGDTGLTVGAGASNLLLRSDT
                     IDGTQNGLLVNGGTNLTIRENTVRGSTRGLRLAGAASGHILANDISGTTVGLDILAAF
                     TGPIEGNNIHGAATGVVYGVGAELSGNRIRNNTVGVASSINDPAQALGFVAETTSKPN
                     EIFDNLTGVSLSNGRMEHQHVYANTTGVTGSGTIGGDTLDYANLIEANSTGVSGFAGI
                     IQFNRFSANGTAVNATSEQRILHNLFYRNTDYGVLASGKNDVRIFNNTFYAPLGDNIR
                     IQSASSEVELQNNILWAMTGYDVHVANDSRSGFFSDYNNLYASETGKVGFFTKDFFDV
                     LDWQADIARFDLHSPGATATNPHWAKPRFVSPLTDDFRIEEPVALQRLTSPSVDAGNA
                     LSDNGQKPSGYTNLLANAGFESGLAGWETTAGATVRSASPVAYEGVNYFFAGAVEGGS
                     ASQIVILSDLGYTGAELDAQDLDVLFGGRIRSAAETPVDRGEVLVMFRDGGGAVLATA
                     LAQAGNTSERWELVGQRAHLPIGTRTLEFVYRANRESGGNADAFFDQGFLYVVSESQA
                     TDQGAYGHAETDVPMSGSPRINLRFPDLYVDWEKQEPMDIRRETFGNTNDSPVRIDLY
                     RDGPDGPTWVANIVETTPDSGQFRWIPNAQGVDFGTDGLRVQVTLVSNSAVLDRSQES
                     FTVPEDGTTYYVDDGSNVGDESTQTATGDNRHTGKSAAAPKPNPWNLFHTYDLQAGAA
                     LYVDTGDYPMIYSLGLSGSTDRGIGLDEGFLMAGPAGDGVAATLHPAVPTDRDWALID
                     LDDADFVSIRSLHLRDALRGILVQNDSVDLDLDDVEAWGHTREGIKVETSAALKTWDR
                     ITVHNNGDTGLLVSGPLGGMSQVLAYSNAGYGLYATGGLPSLMGSEFHHNTQDGAYLS
                     STAAIIVQASSFHDNGATGLTLHGSTGSRVGASDLSLGLGNTVYNNAHDGMRVSGDNS
                     GDVLVAGNAAWGNGQDGIEVAWGEAQANVAYNNRTGVSVSSGTAAYNRAYRNTAEGIN
                     VYSSTATGNVAYNTATGLYLQGSNDEARNNLSYANSTA"
BASE COUNT          740 a         1254 c         1178 g          608 t
ORIGIN      
        1 ttcgatagct ccgacagacc ggcccatccg cagctcgcat caaacggcgg agccatggag
       61 ctggcatggg tccgcacgcc cctgaaggac gggcctacca acctgtatgc ccgtatctgg
      121 aacggcactc acttcgtgga gcagataccc ggcgaggccc aggaaccggg cgtcagtgtc
      181 agcggagacg aggctcaggg cctctcgctc gccttggacg cgagcggcag gcccatcgtg
      241 gcgtggcagg aggcccagga ggggagcccc gagatcttcg tgcgcgccac ccagttcgac
      301 ctcggcacga tctacacggc cgacggcacc ccgggccaca cgctccagga gacgctggac
      361 agccacgatc tcggtgcggg tgacgtaatc gtcgtcgccg gcacccaggc aggcttcacc
      421 gtgtccggcg acgacgccgg agtgctcatc gcgggagcgc cgggtgccgt catcacgggc
      481 aacgtgatca tcgccgcggc ggccacgagc gtgacgctgc agcgtcttct catccagggc
      541 agtctcgacc tcgctgccga cggcgtcaca gtgacggagt cggaggttcg gggcggtacg
      601 agcctcaccg gtgggagcgg acaagccatc gtgtatagtc gtctcgcggg ggacaccggc
      661 cttaccgtgg gagcaggtgc ttccaacctt ctcctgcgct ccgacaccat cgacggaacc
      721 cagaacgggc tgctggtaaa cggcggcaca aacctcacca tccgggagaa taccgttcgc
      781 ggcagcacga ggggtctacg ccttgcgggg gctgcatccg gacacattct cgccaatgac
      841 atttccggca cgacggtcgg gctcgacatc cttgccgcgt tcacagggcc catcgaggga
      901 aacaacatcc acggcgctgc caccggtgtg gtctacggtg tgggcgccga actgtcaggg
      961 aaccggatca gaaacaacac ggtgggtgtc gcatcgtcca tcaacgatcc ggcccaagcc
     1021 ctcggattcg tggcagagac cacatcaaag cccaatgaaa tctttgacaa cttaacaggt
     1081 gtctccctca gcaacggccg catggaacac cagcacgtct atgccaatac aaccggcgta
     1141 accggttcgg gcaccatcgg aggcgacacg ctcgactacg ccaacctcat tgaggccaac
     1201 agcaccggcg tctccggctt cgcgggcatc atccagttca accgcttctc agccaacggc
     1261 accgccgtga acgccacctc ggagcagcgc atcctgcaca acctcttcta ccggaacacg
     1321 gactacggtg tgctcgcctc cggcaagaac gacgttcgca tcttcaacaa caccttctac
     1381 gccccactgg gcgacaatat ccgcattcaa tccgcatcca gcgaggtgga gcttcagaac
     1441 aacatcctct gggccatgac gggctacgac gtccacgtgg ccaatgacag ccggagcggg
     1501 ttcttcagcg actacaacaa cctctatgcc tcggaaaccg gcaaggtcgg gttctttacc
     1561 aaggacttct tcgatgtcct cgactggcag gcggacattg cccggttcga tctccactcc
     1621 ccgggcgcca ctgcgaccaa cccgcattgg gccaagcccc gcttcgtgag cccgctcacc
     1681 gacgacttcc gtatcgagga gcccgtggcc ctgcagcggc tcacgagtcc aagcgtcgat
     1741 gccgggaacg cgctttccga caacggccag aaaccgagcg ggtacacgaa cctgctcgcc
     1801 aacgcgggct tcgagtccgg tcttgcggga tgggagacga ctgcaggcgc gaccgtgcgc
     1861 tccgcaagcc ctgtggcgta tgagggggtg aactacttct ttgcgggtgc agtggagggc
     1921 gggtctgcca gccagattgt gatcctctca gaccttgggt acaccggagc cgagctggac
     1981 gcacaggatc tcgatgtcct cttcggcggt cgcatccggt ccgccgcaga gaccccggtg
     2041 gatcgcggcg aagtcctggt gatgttccgg gatggcggcg gggccgttct ggcaacggcg
     2101 cttgcgcaag caggcaacac cagcgagcgc tgggagttgg tgggccagcg tgcccatctg
     2161 ccgataggga cccgtaccct ggaattcgtg taccgtgcca accgggaatc gggcgggaac
     2221 gcagacgcct tcttcgacca gggcttcctg tatgtcgtct ccgagagcca ggccaccgac
     2281 cagggcgcat acggccacgc cgagacggat gtgccgatga gcggctcgcc ccggatcaac
     2341 cttcgcttcc ccgatctcta tgtggattgg gagaagcagg aacccatgga cattcggagg
     2401 gagaccttcg ggaacacgaa cgactcgccg gttcgcatcg acctctaccg ggacggcccg
     2461 gacggtccga cctgggtggc taacatcgtc gagacgacgc ccgattccgg ccagttccgg
     2521 tggataccca atgcgcaggg cgtggatttc ggcacggacg ggctccgcgt ccaggtcacc
     2581 ctcgtcagca attcggccgt cctggatcgg agccaggaga gctttaccgt accggaggat
     2641 ggcacgacgt actatgtgga cgacggctcg aacgttgggg acgagtccac gcagacggcg
     2701 acgggcgaca accgccatac cggcaagtcc gccgcggccc ccaagcccaa cccctggaat
     2761 ctcttccaca cctacgatct ccaggccggg gcggccctgt atgtcgacac gggcgactat
     2821 ccgatgatct actctcttgg cctgtcggga agcacggatc gcggaatcgg cctcgacgag
     2881 ggctttctca tggccggccc ggcaggcgac ggggtggcgg caaccctcca tcccgccgtc
     2941 ccgactgacc gggactgggc cctcatcgat ctcgatgatg cagacttcgt ctcgatccgc
     3001 agcctccacc tgcgcgatgc cctgcggggg atcctcgtcc agaacgactc agtcgacctc
     3061 gatctcgacg acgtggaggc gtggggccac acccgcgagg gcatcaaggt tgagaccagc
     3121 gctgccctga agacctggga tcgcatcacg gttcacaaca acggcgacac cggcctactg
     3181 gtgagcggcc cgctcggtgg gatgtcccaa gtcctcgcct attcgaatgc cggctatggt
     3241 ctgtacgcca caggtggtct gccgagcctc atggggagcg agtttcatca caacacgcag
     3301 gacggcgcct acctgtcgag cacggccgcc atcatcgtgc aggccagcag cttccacgac
     3361 aacggcgcta ctggccttac cttgcacggc tccaccggaa gccgggtagg agcgagcgat
     3421 ctctccctgg gtctggggaa tacggtgtac aacaacgctc acgacggtat gagggtgagt
     3481 ggagacaaca gcggggacgt cctcgtggcc ggaaacgcgg cctggggcaa cggccaggac
     3541 ggtatcgagg tcgcctgggg cgaggcccag gccaacgtgg cttacaataa ccgcacgggc
     3601 gtctccgtca gctccggcac ggccgcatac aatcgggcgt atcgcaatac ggcggaggga
     3661 atcaacgtct actcctccac ggcgacgggc aatgtggcgt acaacaccgc caccggactg
     3721 tacctgcagg ggagcaatga cgaggcacgg aacaacctga gctatgccaa cagcaccgca
//
LOCUS       DBNGZC010000335         3746 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2081759, whole genome shotgun sequence.
ACCESSION   DBNGZC010000335 DBNGZC010000000
VERSION     DBNGZC010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2081759"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="AC16G0_06070"
     CDS             <1..765
                     /locus_tag="AC16G0_06070"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5099226.1"
                     /translation="QMIPLSTLVSISNIAGTELTTRFNLLRSVEIQGAPARGFTSGQA
                     LAALEQVFGETMPKEMGFAYSSVSYQEMVAPPSAPTFIMAIVCVFLLLAALYESWRLP
                     WAVLLGSPLVALGAFFGVWLMGYDNNVYVQIGLVMLIGLAAKNAILIVEFAKAKHEEG
                     MSLEESALTSARLRFRPILMTAFAFILGVVPLMRASGAGAGAQNVMGTGVFWGMLIAT
                     GLGVFIIPGNYTFLEGLGRRKAAKPAVSVPSPEGAH"
     gene            767..2188
                     /locus_tag="AC16G0_06075"
     CDS             767..2188
                     /locus_tag="AC16G0_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HIR5099227.1"
                     /translation="MTRRPIHILCAVSLVATGCTLGPDYRRPEIEVPAAYRGAEAETL
                     GPSVSLGELDWWTIFQDPELQELIRTTLAANYDLRVAVSRILQAQSQLTITRSQQFPT
                     VNAQADAPYTGLLGGNRPPTVARETFTPEGGLTFAWELDFWGKFRRATEAARAQLLAS
                     EEVRNGVVTSLVAQVAQAYFSLRTLDLDLEISRRTVDSRQQSVELVRARLDGGVAGIL
                     DLQQAETLLYTATKFIPDTERQIEETENLISILQGKYPGPIPRGRPLGQQLVLPKVPS
                     GLPSDLLARRPDVRQAEEQLVAANAQIGVATTQFYPQVLLSGFGGVNGFVISGQSFGP
                     FGFLSALPVISFPLFNAGRAQAGVDLAEAQTQEAVIRYQQTIQQAVREVSDALVDVRK
                     LQEVRIEQEKLTQTLADASEVARQRYEGGVSSYLEVLDTERQFFQAELDLTTAQLNEM
                     SAFVRLYKAVGGGWQPEQKPNTP"
     gene            2276..3049
                     /locus_tag="AC16G0_06080"
     CDS             2276..3049
                     /locus_tag="AC16G0_06080"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5099228.1"
                     /translation="MNIPKSDLIASIARAKGELDQALSALAALPAFDPGTVGYAAHAI
                     NNYLMLSNATIDLLESALADHLNPDVRSLLATLRQSAVMMDHTVNQLINASADGEPCL
                     AFGSVNVVRLIRIGCEYYQALAARKQILILFASVVEAAYVWTDRVAVGAILDNLLSNA
                     VKYSPPGKNVRVMVTQGPNDLVCHVQDEGPGLSAEDQAQLYRRGVRLSPQPTGGEASS
                     GFGLAIAKDLIEWLGGKIWCESSPGQGARFSFSLPTGSR"
     gene            3558..>3746
                     /locus_tag="AC16G0_06085"
     CDS             3558..>3746
                     /locus_tag="AC16G0_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR5099229.1"
                     /translation="MRRGILKGCAVVTLVALVGTGCAAIEANPKTTIGAAGGATVGGL
                     IAAAAGGNPAAIAASVIGG"
BASE COUNT          635 a         1175 c         1258 g          678 t
ORIGIN      
        1 cagatgatcc ccctctccac gctggtcagc atcagcaaca tcgcggggac cgagttgacg
       61 acccggttca acctgctgcg gtccgtggaa atccaggggg cgcccgcccg cggcttcacc
      121 tccggacagg ccctggcggc tctggagcag gtctttgggg agacgatgcc caaggagatg
      181 ggcttcgcct actcctcggt ctcgtaccag gaaatggtcg ccccgccttc cgcgccgacc
      241 ttcatcatgg ccatcgtgtg cgtgttcctg ctgctggccg cgctgtacga gagctggcgg
      301 ctgccctggg cggtcctcct gggatcgcct ctggtggcgc tgggcgcctt cttcggcgtc
      361 tggctgatgg gctacgacaa caacgtctac gtgcagatcg gcctggtgat gctgatcggc
      421 ctggcggcca agaacgccat cctgatcgtg gagttcgcga aagcgaagca cgaggagggc
      481 atgtccctcg aggagtccgc cctgacatcg gcccgcctgc ggttccggcc gatcctgatg
      541 acggcgttcg cctttatcct gggggtcgtg ccactgatgc gggcgagcgg cgcgggcgcg
      601 ggggcccaga acgtgatggg caccggcgtg ttctggggga tgctgatcgc caccgggctg
      661 ggtgtgttca tcattccggg caactacacg ttcctcgagg gactcggccg ccgcaaggcc
      721 gccaagcccg cggtaagcgt gccttcgccc gaaggagcgc actgagatga cccgccgacc
      781 gattcacatt ctttgcgctg tgtctctggt cgctactggc tgcaccctcg gcccggacta
      841 ccgccgtccc gagatcgagg tgccggcagc ctatcgcggg gcggaggccg aaaccctggg
      901 gccgagtgtg tccctggggg aactcgactg gtggacgatc ttccaggatc cggagctaca
      961 ggagctgatc cgaacaaccc ttgccgcgaa ctacgatctc cgggtcgcgg tgtctcgaat
     1021 cctccaggcg cagtcccaac tgacgatcac ccgttcccag cagttcccga cggtgaatgc
     1081 tcaggctgac gctccgtaca ccggcctcct ggggggcaac cggccgccga cggttgcccg
     1141 ggagaccttc acgccggagg ggggtctcac atttgcctgg gagctggact tctgggggaa
     1201 attccgccgc gccaccgagg cggcccgggc ccaactgctg gcgtccgagg aggtccggaa
     1261 cggcgtggtc acgagcctgg tcgcgcaggt ggcccaggcc tacttcagct tgcgaacgtt
     1321 ggacctggat ctcgagatct ccagacgcac ggtggactcg cggcagcagt cggttgagct
     1381 ggtcagggcc cggctggatg gcggggtggc cggcattctc gatctccagc aggccgagac
     1441 cctcttgtac acggcaacga aattcatccc ggacaccgag cgccagatcg aggagaccga
     1501 gaacctcatc agcatcctgc agggaaagta cccaggaccc atcccccgcg ggcgcccact
     1561 cggccagcaa ctcgtgcttc cgaaggtgcc atccgggctt ccctcggatc tgttggcccg
     1621 gcgcccggat gtccgccagg cggaggaaca gctcgtcgcc gccaacgcgc agatcggcgt
     1681 cgccacgacg cagttctacc cccaggttct cctctcgggg ttcggcgggg tcaacggctt
     1741 cgtgatcagc gggcagtcct tcggcccctt tggcttcctc agcgccttgc cggtcatcag
     1801 cttcccgctc ttcaacgccg gccgcgccca ggccggcgtg gacctggcgg aagcgcagac
     1861 ccaggaggca gtgatccgct atcagcagac gattcagcag gccgttcgcg aagtctcgga
     1921 cgccctggtg gatgtccgga agctgcagga ggtccggatc gagcaggaga agctcaccca
     1981 gaccttggcg gatgccagcg aggtggcccg gcagcgctac gagggaggtg tgtcgagcta
     2041 cctggaggtg cttgacaccg agcgccagtt cttccaggcc gagttggacc tcacgacagc
     2101 tcaactgaac gagatgtcgg ccttcgtccg gctgtacaag gccgtcggcg gtggttggca
     2161 acccgagcag aagccaaaca ctccgtaagg tgggtgccta tcgaatgcct tgcccccggc
     2221 aacaccagac tgagggggcg aggtgtcgct gacgtgagac agagcgaggc atggcgtgaa
     2281 catcccgaaa agcgacctga ttgcctcgat agcacgtgcg aagggcgaac tggaccaagc
     2341 gctcagcgcc ctggcggccc ttccggcctt cgatcccggg accgttggct acgccgccca
     2401 tgcgatcaac aactacctga tgctcagcaa tgccacgatt gacctgctgg aatcggctct
     2461 ggcggaccat ctgaaccccg acgtgcgttc cttgcttgcg acattgcggc agagcgcagt
     2521 gatgatggac catacggtca accaactgat caatgcgtcg gcggatggag agccgtgtct
     2581 agccttcggc agtgtgaacg tggtgaggct gattcggatc gggtgcgagt actaccaggc
     2641 cctcgcggcg cgcaagcaga tcctcatcct gttcgcgtct gtcgtcgagg cggcctacgt
     2701 gtggaccgat cgggtcgccg tgggggcgat tctggataac ctgctgtcca atgcggtaaa
     2761 atactcaccg cccgggaaaa acgttcgggt gatggtgacc cagggtccca acgacctcgt
     2821 gtgccacgtg caagatgaag ggccgggcct gagtgcggaa gatcaggcgc aattgtatcg
     2881 ccggggcgtg cggctcagcc cacaacccac ggggggcgag gcgtcatcgg gcttcggcct
     2941 ggccatcgcc aaggacctga tcgagtggct gggtgggaag atctggtgcg aaagctcccc
     3001 gggccagggg gcgcgcttct cgttttccct gcccacggga agccggtaga ggggtgctca
     3061 gcgcacgaag cctccggtcg tcgaggggaa gaagcccggt cgcatcctga cgctggatcg
     3121 aagcgatcgc cggatgccca acggggacga gacacggcga ggatggaggc agaagctcgc
     3181 cagcgagatg gcggcgtact ggatcacggt cctttatctg cctgtttttt cgggatgttc
     3241 attgcgtacc aacgactgat actggcccag catgccatca gctttgagga gttcgtgcga
     3301 cgggacaggt atgagttact cgcccgatgc ctggtccttt tcttcgcctt catcccttct
     3361 tcgctgtcaa ggagctaggg cgggtgctgg gtagggggag gatcctcgga ctgtcctttc
     3421 ggtgccgagt gccgaggaaa gctgctcggt ccatcctctc aggatccgcg tgaggactgt
     3481 cgtcgtgccc atcggtctcc ggcggctcga cgtcacacgg gtcgtttcaa cttgcagtcc
     3541 acaaaggaga tgtggtcatg cgcaggggta ttctcaaggg gtgtgcggtg gtcacacttg
     3601 tcgccttggt tggcacaggc tgtgccgcca ttgaagcgaa ccccaaaacg accatcgggg
     3661 ccgcgggggg tgccacggtg ggcggcctga ttgccgccgc ggccggcggg aatccggcgg
     3721 cgattgccgc cagcgtgatc ggcggc
//
LOCUS       DBNGZC010000336         1975 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1494723, whole genome shotgun sequence.
ACCESSION   DBNGZC010000336 DBNGZC010000000
VERSION     DBNGZC010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1494723"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..1705)
                     /locus_tag="AC16G0_06090"
     CDS             complement(122..1705)
                     /locus_tag="AC16G0_06090"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HIR5099230.1"
                     /translation="MAKHTTHRHGGEHVDREVAHKPRTNAWLLHAVVFVCGAVLMGLE
                     IVGSRILAPYFGNSIFVWGSLISVVLAALSLGYWLGGMVADRFPRLSVMGGLIALPGV
                     MIALLPFIYPPLNGAIAASDLGARLSPLLSSLLLFLVPSVCLGTISPFAVRLQAKAVA
                     SVGTTAGGLYAVSTAGSIAGTLLTAFYLIAVVGVANIVHGLGLTLLLLAAVIFVGGRR
                     FGRAVLAIGLAGLMLLGLVWRAQTLAADPGLLLDTDSFYNHIRVAEDSGTRYMDFDNL
                     RQSAMLLQDPWELRLRYTRFLALALTFQPDPKRILILGLGGGSFPKRLYHDFPQTVVD
                     VADIDPKVVTVAKRYFQVPEDDRLHLIVQDGRRFVRETKATYDLIFLDAYNSDTIPFH
                     LTTREFYQELKARLAPGGVVVSNIIGSLRGPESAFFRAMYRTLTDALPTVYVIPTHGG
                     AGEVPLGEINIILLATRDKVRLTRAELMARVGQVGGKLVPASDLAEYASHLLEIPVDA
                     GDVPILTDDYAPVEILRAS"
BASE COUNT          384 a          633 c          662 g          296 t
ORIGIN      
        1 tgctcaaaaa ggttcagatg caaggcggcg catgaggcga ggcgcgaggc gtactgagtc
       61 tgtacgccgc agcgccgcgc gaatgcgcca acgtagcaga tgggcctttt tcagcagcct
      121 gtcaggaggc tcggaggatc tcgactggag cgtagtcgtc ggtcaggatc ggcacatccc
      181 cggcgtccac cgggatctcc agcagatggg aggcgtattc cgccaggtcc gaggctggga
      241 ccagcttgcc cccaacctgc cccacccggg ccatcagctc cgcccgggtc aggcgcacct
      301 tgtcccgcgt ggcgagcaga atgatgttga tctcgccgag cggcacttcc cctgccccgc
      361 catgggtggg gatcacgtac accgtgggaa gggcgtccgt cagcgtccgg tacatggccc
      421 ggaagaaggc gctctcgggc ccgcgcaggc tcccgatgat gttggacacc accacccccc
      481 cgggggcgag ccgggccttg agctcctggt agaactcccg ggtggtcagg tgaaaaggaa
      541 tcgtgtcgga gttgtaggcg tccaggaaga tcaggtcgta ggtagctttc gtctcgcgaa
      601 cgaagcgccg gccgtcctgg acgatgaggt gcagccggtc atcctcgggt acctggaaat
      661 agcgcttggc aacggtcacg acctttgggt cgatgtccgc cacgtccacc acggtttgcg
      721 ggaagtcatg gtagaggcgc ttgggaaacg acccgccccc cagacccagg atcaggatgc
      781 gcttgggatc gggctggaac gtcaaggcca gggccaggaa gcgtgtgtac cggagccgca
      841 gctcccacgg atcctggagc agcatggcgc tctgccgcag gttgtcgaaa tccatgtagc
      901 gtgtcccgga atcctcggcc acgcgaatgt ggttgtagaa ggagtccgtg tccaagagca
      961 gccccgggtc cgccgccagg gtctgggccc gccagaccag gccgaggagc atgagacccg
     1021 ccaagccgat cgccagcact gcacgcccga accgccgccc cccgacgaag atcaccgccg
     1081 ccagcaggag cagcgtgagg cccaggccgt ggacgatatt ggccacgccc acgaccgcga
     1141 tgaggtagaa ggccgtgagc agggtccccg cgatgctccc ggcggtggag acggcataca
     1201 ggcctcccgc ggtcgtgccg accgaggcta cggccttggc ctgcagtcgc acggcgaagg
     1261 gcgagatggt acccaggcac acgctgggga ccaggaacag gaggaggctg gagagcaggg
     1321 ggctgagccg cgctcccagg tcactcgccg cgatggcgcc gttcaagggg ggatagatga
     1381 agggcaggag ggcaatcatg acccctggga gggcgatgag gccgcccatg accgagaggc
     1441 gcggaaaacg atccgccacc atcccgccca gccagtagcc cagactcagg gctgccaaaa
     1501 cgacgctgat gaggctcccc cacacgaaga tggagttgcc gaagtatggg gccaggatcc
     1561 ggctccccac gatttccagc cccatgagga ccgccccgca gacgaagacc accgcgtgga
     1621 gcagccaggc gttggtccgc ggcttgtgcg cgacttctcg gtcgacgtgt tcccccccgt
     1681 gacggtgcgt cgtgtgcttg gccatcgagg catgatcctt tcctgctctc ccgagttgca
     1741 gctccgcgaa cataaactag gcctgtgacg gcgccccgag cacgccgggc ccgcgggtca
     1801 gccgggttcg ggacgccgtg gcgcgaatcc tccaaactga actctgatcc gaaattcggc
     1861 gtctcgccaa gacgcaaaga tcgccaagac ccacgatcat aatctgggca cagcaggcaa
     1921 gagacccacc gcccctggaa ggagtcatcc gatatgggtg attccgtgat taaat
//
LOCUS       DBNGZC010000337         7304 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_125454, whole genome shotgun sequence.
ACCESSION   DBNGZC010000337 DBNGZC010000000
VERSION     DBNGZC010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7304)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7304)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7304
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_125454"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="AC16G0_06095"
     CDS             complement(<1..473)
                     /locus_tag="AC16G0_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012676959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HIR5099231.1"
                     /translation="MSRPLADINKIVRTLRSTTRQWDPTAVGAVAKGSRDPFRILISC
                     LISLRTKDQVTHEASSRLFRLARTPRGMAALPVSRIARTIYPAGFYRTKARTIKELSR
                     RLIQRHGGKVPDDLDTLLTLKGVGRKTANLVVTMAFGKPGICVDTHVHRISNRLG"
     gene            559..1053
                     /locus_tag="AC16G0_06100"
     CDS             559..1053
                     /locus_tag="AC16G0_06100"
                     /inference="COORDINATES: protein motif:HMM:NF027971.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5099232.1"
                     /translation="MRIFFAGAARTVTGTQHLLEFDGTHLLLDCGLYQGRRKDSSERN
                     RHLPYDPRQVNSMILSHAHIDHSGNIPTLVKQGFPGNIICTPATRDLCSVMLRDAAHI
                     QEADADFMNRKHRDQLEEPVIPLYTEADVLHALQHFRSLDYHRPTQILPGVTLTFTDA
                     GHVL"
     gene            1105..1950
                     /locus_tag="AC16G0_06105"
     CDS             1105..1950
                     /locus_tag="AC16G0_06105"
                     /inference="COORDINATES: protein motif:HMM:NF022449.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HIR5099233.1"
                     /translation="MFSGDLGRRNMAILRDPEVPERPDVLILESTYGDRLHTPMAGME
                     AQLAEVVNRTVARKGKILVPSFSLERTQEFVYALHRLRLRKAIPSIPIYVDSPLSIRI
                     TEVFKLHPECFDEDTLAFMRVNGDPFGFDNLVYVSTAEESKALNDREGPWMVIAASGM
                     CEAGRILHHLRNSVEDARNTVLIIGYQAQHTLGRRIAEHQPEVRIFGIEHPLEAEVVV
                     MDAFSAHADRDDLLDFVHRCQPSLRKVLLVHGEEEQMQPLAERVTRMGIPDVHIPMLG
                     EFAQI"
     gene            2134..2835
                     /locus_tag="AC16G0_06110"
     CDS             2134..2835
                     /locus_tag="AC16G0_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HIR5099234.1"
                     /translation="MEEALHFLTFGPHPDDAEIGTGAFLLKMKNRGYRTGMALLTEGD
                     MGSGTPEIRREETRRAARDLKLDVLEIMNLGDTRLEDSHENRIAVASLVRKYRPEIVL
                     APYYGIEPSRGRGHADHIISGHLVTHGVNFAHLEKYPAEGRPHAVKKILYYFLPPFMK
                     PSLIVPVDEEFPLAIQALANHKSQFDRSDRPRFFPTRLETNAGYLGAQIGAKFGQGFY
                     VQDALKIEDPLPLFK"
     gene            2974..3189
                     /locus_tag="AC16G0_06115"
     CDS             2974..3189
                     /locus_tag="AC16G0_06115"
                     /inference="COORDINATES: protein motif:HMM:NF014171.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter regulatory subunit"
                     /protein_id="HIR5099235.1"
                     /translation="MTVGSALIGKTLADLDLHNRVGLAIIAVRRGPKGQFVYNPAAST
                     RVGAGDALIVCGEPPKIEALRDMWAGR"
     gene            3196..3786
                     /locus_tag="AC16G0_06120"
     CDS             3196..3786
                     /locus_tag="AC16G0_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR5099236.1"
                     /translation="MPTPFNSPEEGAVADELKAWRRVGSRLLHQQGHVVIREDLLELP
                     GGRQLPYPVMGLGASVGILPFLSEDQVILVRQYRHVTGGFCWEVPGGGILPGEPPDLA
                     AQRELREETGYRSACLRRLGGFWPNNAYLDEVIHIYLAEGLTRDPLPADQDEHLEARA
                     FPWTEAVAMAQDDRITCGLTKLAILWATAQPRWSRA"
     gene            3852..4514
                     /locus_tag="AC16G0_06125"
     CDS             3852..4514
                     /locus_tag="AC16G0_06125"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR5099237.1"
                     /translation="MRKWLKRSPPVSEGPATYVIGDIHGCLQSLERLLHKVRPRHEDR
                     VIFIGDYIDRGPQSREVVELLLRLPYPSIFLLGNHEKMLLDYLAGGDASLYLLNGGTA
                     TLESYGGDAANIPQDHLRFFRSLRPIYETPDHVFVHAGIRPLVPLEDQELKDLVWIRQ
                     EFYQFVGRFPKPVVFGHTPMRHVLLAEDRIGIDTGCVYGGKLTCLKLPEREIIQVSGW
                     RD"
     gene            complement(4665..6164)
                     /locus_tag="AC16G0_06130"
     CDS             complement(4665..6164)
                     /locus_tag="AC16G0_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HIR5099238.1"
                     /translation="MGKLPLQTRVEKLTALLEVGKAMASERSLDRLLRLILNEVTTVM
                     EAERSSLFLVDRETNELWSKIAQGLEVREIRIKIGMGISGYVAQTGKILNIQDAYGDP
                     RFNQETDRRTGYRTRTILCAPMLNQRNEVIGVLQVLNKRDGVFTAEDEELLLAISSQA
                     AVAVENAILYEDIQKLFEGFIKASVYAIESRDPTTSGHSERVAILSVGLAEQVNRHDS
                     GPYADVSFSPEDIRELRFASLLHDFGKVGVREPVLVKANKLYDRDLDLIVCRFNLIKR
                     SLQFDALQTKLGLVTSLGPGSEAQLADVDAELQRRLAEVDGQLRFILEANKPTGLPKG
                     GFERILEIAAHTFMDADGNRLPFLTSREAENLSIAKGSLTVEERLQVESHVTHSFRFL
                     RQIPWTRDLRRIPPVAYAHHEKLNGTGYPSGLRGDDIPVQAKMMAICDIYDALTAADR
                     PYRRAVPPEQALGILGLEGKNGFVDAELVRLFTEGKVWALTAGLRTAVA"
     gene            complement(6355..>7304)
                     /locus_tag="AC16G0_06135"
     CDS             complement(6355..>7304)
                     /locus_tag="AC16G0_06135"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5099239.1"
                     /translation="IGLTGLASEGTRRRVDYAGTACLVVSIIALMLSLLEVGRVGRPF
                     GAEALAGLAIFLTFTAVFLWIESRAPEPILPLGLFGRRIFTTTTACLFLSGVAMFGAV
                     SFIPLFVQGVLGGTAIQAGSVLTPTSLSWTVGSALGGRLLNRLGYRTLAVAGMLSLTT
                     GYFLFTRLGAGSPLHHAAEIGAILGLGMGMITVTTVVAVQTAAPPGQIGVVSTLPFFF
                     RNIGATIGVAIMGTILNAHVVGAGGGPVSLGGPEGSFEALPTLLRGQLADGIRAAFRF
                     GFIAVALGLPVSLLVPHLSPARPAEKEGAPVPSSGSLIE"
BASE COUNT         1308 a         2364 c         2410 g         1222 t
ORIGIN      
        1 attcccagcc ggttgctgat gcgatggacg tgggtgtcca cgcagatccc gggctttccg
       61 aaggccatgg tgacgacgag gttggcggtc ttgcgaccaa ctcccttcaa cgtgagcagg
      121 gtgtccaagt catccggcac cttgcccccg tgccgctgga tcagcctgcg ggagagttct
      181 ttgatcgtcc tggccttggt gcggtagaag ccggcgggat agatggttcg cgcaatcctg
      241 ctcacgggca aggctgccat gccgcgaggc gtcctggcca ggcggaagag acggctggag
      301 gcctcgtgcg tcacctggtc tttggtgcgg aggctgatga ggcacgaaat caggatccga
      361 aatggatctc gcgagccctt ggcgacagcg ccaacggcgg tgggatccca ttgtcgggtg
      421 gtgctccgga gggtccgaac gatcttattg atgtccgcga gtggtcttga cattgtcctc
      481 ccatccgatt gagtgaccgg ggttgaacgg cggctgagta tagcatgagg ggcgggtgcg
      541 cgagagagga ggagagcgat gcgcattttc ttcgcgggag ccgcacgcac cgtgaccggg
      601 acgcagcacc tcctggagtt cgacgggacg cacctgctcc tggactgcgg gctgtaccag
      661 gggcggcgca aggattcgag cgagcggaac cgccacctgc cttacgaccc gcgccaggtg
      721 aattccatga tcctgtccca cgctcatatc gatcattccg gcaacattcc cacgctggtc
      781 aagcaggggt ttccgggcaa catcatctgc acccccgcca cccgcgacct gtgtagcgtg
      841 atgctgcggg atgcggccca catccaggag gcggacgccg acttcatgaa ccggaagcac
      901 cgggaccagc tggaagagcc ggtgatcccg ctctataccg aggcagacgt cctgcacgct
      961 ctgcagcatt tccggagcct ggactaccat cgtccgaccc agatcctccc gggcgtgacc
     1021 ctgacgttca cggatgccgg gcacgttctg tgatcggcca tcgtccagct cgacatcgac
     1081 cggggacgcg atcggctcag gctcgtgttc agcggggacc tgggccgacg gaacatggcg
     1141 atcctgcgcg accccgaggt cccggagagg ccggacgtcc tcatcctgga gagcacctac
     1201 ggtgaccggc tgcacacgcc catggccggg atggaggcgc agttggccga ggtggtcaat
     1261 cgaaccgtgg cccggaaggg gaagatcctg gtgcccagct tctccctgga acggacccag
     1321 gaattcgtgt atgcgctgca ccgactcagg ctgcggaagg ccatcccatc catccccatc
     1381 tacgtggaca gcccgctctc gatccggatc accgaggtct tcaagctgca ccccgagtgc
     1441 ttcgacgagg acacactggc cttcatgcga gtcaacggcg atcccttcgg cttcgacaac
     1501 ctcgtgtacg tcagcaccgc cgaggagtcc aaggccctga acgatcgaga gggtccctgg
     1561 atggtcatcg ccgcctcggg gatgtgcgag gcggggagaa ttctgcacca cctgcggaac
     1621 tcggtggagg acgcccggaa caccgtcctg atcatcgggt accaggccca gcacaccctg
     1681 ggccgcagga tcgccgagca ccagcccgag gtccggatct tcgggataga gcatcccctg
     1741 gaggctgagg tggtggtcat ggacgccttc agcgcccacg cggaccgcga cgacctcctg
     1801 gatttcgtgc atcggtgcca gccgtccctg cggaaagtcc tcctggtcca cggcgaagaa
     1861 gagcagatgc agcccttggc cgaacgggtc acccgcatgg gcattcccga cgtgcacatc
     1921 cccatgctcg gggagttcgc gcaaatctga tgccgccggc aagcggggat ggtcgctcgt
     1981 ggattgactt tttgctcctc cccccataat cttccctgcg ttctgccgga ccgaccgcgc
     2041 ggccgtgagg cccgccaggg accggcggca gcctgacgcg tgaccagaac ccttccgcca
     2101 ataccggcgg cgacacggaa gagaggacaa cgcatggagg aggcactgca ctttctgacc
     2161 ttcggcccgc atccggacga cgccgagatc gggaccgggg catttctgct gaagatgaag
     2221 aaccggggct accgcaccgg aatggctctc ctgaccgagg gggacatggg cagcggcacg
     2281 cccgagatcc ggcgcgagga gacgcggcgg gcggcccgcg acctcaagct ggatgtcctg
     2341 gagatcatga atctggggga cacccgcctg gaggactccc acgagaatcg gatcgccgtg
     2401 gcctccctgg tgcggaagta ccggccggag atcgtgctgg ccccgtacta cgggatcgag
     2461 ccgagccgcg gccggggtca cgcggatcac atcatctccg ggcacctggt gacccacggg
     2521 gtcaacttcg cccacctgga gaagtacccg gccgaggggc gaccgcacgc ggtgaaaaag
     2581 atcctctact atttcctgcc tcccttcatg aagccgagcc tcatcgtccc ggtggacgag
     2641 gagttccccc tggccatcca ggccctggcc aatcacaagt cgcagttcga tcgatcggac
     2701 cggccgagat tctttcccac ccggctggag accaacgccg gctacctggg ggcccagatc
     2761 ggggcgaagt tcggtcaggg tttctacgtc caggacgcgc tgaagatcga ggacccgctg
     2821 ccactcttca agtgacggga ttccatggcc gagcgcacgg ccggatcagt gccgcctgct
     2881 gcccgaccga ggggtcccgt ccggcatggg cctgcaagac tggacctgat gctgcgggat
     2941 catcaccgcg ccttgcggtg tgaagaggcc gaagtgacgg tcgggtcagc gctcatcggg
     3001 aagacgctgg ccgacctcga cctccacaat cgggtggggt tggcgatcat cgccgtccgc
     3061 cgagggccca aagggcaatt cgtgtataat ccggcggctt ctacccgggt cggggcaggg
     3121 gacgcgctga tcgtctgcgg cgaacccccc aagatcgagg cgttgcgaga tatgtgggcg
     3181 gggaggtagc ggagaatgcc aaccccgttc aactctccgg aggagggggc ggtggcggac
     3241 gagctgaagg cctggaggcg ggtgggaagt cggctgctgc accagcaggg tcacgtggtc
     3301 atccgggaag atctgctgga actccccggc gggcggcagc tcccctaccc ggtcatgggg
     3361 ctgggggcaa gcgtcggcat ccttcccttc ctctccgagg accaggtgat cctggttcgg
     3421 caataccgtc acgtgaccgg cggcttctgc tgggaggtcc cgggaggcgg gatcctgccc
     3481 ggcgagcccc cggacctggc cgcacaacgc gaactccgcg aggagaccgg gtatcgctcc
     3541 gcctgcctgc ggcggttggg cggcttctgg ccgaacaacg cctatctgga tgaggtcatc
     3601 cacatctatc tggccgaggg actcaccaga gacccgctcc cggcggacca ggacgagcat
     3661 ctggaggcac gcgccttccc ctggactgag gcggtcgcca tggcgcagga cgatcgcatc
     3721 acgtgcgggc tcaccaagct cgccatcttg tgggcgacgg cacagccgag gtggtcgcgg
     3781 gcctaggaat ttcaggagac gctgacggcg tgccgatccg gcgtgcgcgg cgttcttcgg
     3841 gaagagtgcg gatgcggaag tggctcaaga ggtcgccgcc ggtctcggaa ggtccggcga
     3901 cctacgtcat cggagatatc cacggatgtc tgcagtcgct cgagcggctg ctgcacaagg
     3961 tccggcctcg tcacgaggac cgggtgatct tcatcgggga ttacatcgac cgcggaccgc
     4021 agtctcgcga ggtggtggag ctgctcctgc ggctgcccta cccttccatc ttcctgctgg
     4081 gcaatcacga gaagatgctg ctggactatc tggctggcgg cgacgcgtcc ctgtatctcc
     4141 tcaacggcgg cacggccacg cttgagagct acggcggcga tgccgccaac atcccccagg
     4201 accacctgcg cttcttccgc agcctgcggc cgatatacga gacgcccgac catgtcttcg
     4261 tgcacgccgg tattcgaccc ctggttcctt tggaggacca ggagctgaag gacctggtgt
     4321 ggatccgcca ggaattttac caattcgtcg gacgcttccc caagccggtg gtcttcggcc
     4381 acacacccat gcgccatgtc ctgctggccg aggatcgtat cgggatcgac accggatgcg
     4441 tttacggggg aaagctcacc tgcctgaagc tccccgagcg cgagatcatc caggtctccg
     4501 gatggcgcga ctgaccccgc gcctccacca cagagcacac cgcccgtgcc ctcaaccacg
     4561 gatttcccaa attccgcaga tcctttggaa tggattccct tcgcgaaatc tgcggtatct
     4621 gcagtcaaca cgtcgggagg ggtttgccgg tcggtctcgg caggctaggc aaccgcagtg
     4681 cgcagaccag cggtgagtgc ccagaccttg ccctcggtga acaggcgcac cagctccgcg
     4741 tccacgaacc cgttcttccc ctcgagcccc aagatcccca gggcttgctc gggtggcacg
     4801 gcgcgcctgt acggacggtc ggcggccgtg agcgcgtcgt agatgtcaca gatcgccatc
     4861 atcttggcct gcaccgggat atcgtcgcct cggaggccgc tcgggtagcc ggtgccgttc
     4921 agcttctcgt ggtgggcgta ggcgacggga gggatgcgcc gcaggtcccg ggtccacggg
     4981 atctggcgga ggaagcggaa gctgtgggtc acatgggact caacctgcag ccgttcctcg
     5041 acggtcaggc tccccttggc gatggagaga ttttccgcct cccgggaggt cagaaagggg
     5101 agccggttgc cgtccgcatc catgaaggtg tgggcggcga tctccaggat gcgctcgaat
     5161 cctcccttgg gaagcccggt gggtttgttt gcctcgagga tgaaccgcag ctgaccgtcc
     5221 acctcggcga gccgccgttg gagctccgcg tccacgtcgg ccagctgcgc ctcggaaccc
     5281 ggccccagtg acgtgaccag accgagcttc gtctgcaggg cgtcgaactg caggctccgc
     5341 ttgatgagat tgaaccggca cacgatcagg tcgaggtcgc ggtcgtagag cttgttggcc
     5401 ttgaccagga ccggctcgcg cacgcccacc ttgccgaaat cgtgcaggag cgaggcgaag
     5461 cgcagttcgc ggatgtcttc gggcgagaag ctgacgtcgg cgtaggggcc ggaatcgtgc
     5521 cgattcacct gctccgcgag tcccacgctg aggatggcca cccgctccga atgaccggag
     5581 gtggttgggt ctcgcgactc gatggcgtac accgaggcct tgatgaagcc ctcgaatagc
     5641 ttctggatgt cctcgtacag gatcgcgttc tccacggcca cggccgcctg actggagatc
     5701 gccagcaata gctcctcgtc ctcggcggtg aagaccccgt cccgcttgtt cagaacctgc
     5761 agaacgccga tgacctcatt ccgctggttc agcatcggtg cgcagaggat ggttcgcgtc
     5821 cggtagcctg tgcgacggtc tgtctcctgg ttgaaccggg gatcgccgta ggcgtcctgg
     5881 atgttgagga tcttccctgt ctgggccacg taccccgaga tgcccatgcc tatcttgatc
     5941 cggatctcgc gcacctccag gccctgggcg atcttggacc aaagctcgtt ggtctcgcga
     6001 tccaccagaa aaagcgaact ccgctccgcc tccatgaccg tggtcacttc gttcaggatc
     6061 aaccgcagga ggcgatccag gctccgctcc gaagccatgg ccttgccgac ttccaaaagc
     6121 gcagtcagct tttccacccg ggtctggagc ggcaatttcc ccatggcacc accctcgtca
     6181 ggagccgcca gcgtatcgga aatgcggggg aagcctaacc gcgatttttc cgggggtcaa
     6241 gccgcgtttt atgccggggc ggaaactcgt gcggagcggc aaaccaactc cggcgaccag
     6301 ttacgggacc cgccggaact tgttgcccgc agaatccatg gtccgcgcag cgggctactc
     6361 gatcaggcta ccggaagagg ggacgggtgc gccctccttt tccgcggggc gggccgggga
     6421 gagatggggg acgagcaggc tcaccgggag gcccagggcc accgcgatga agccgaaccg
     6481 gaaagcggcc cggatgccat ccgcaagctg gccccggagc agcgtgggga gtgcctcgaa
     6541 ggatccctcg ggacccccca gggagacagg accacccccg gcgccgacca cgtgggcatt
     6601 caagatcgtt cccatgatgg ccacccctat cgtggcgcca atatttcgga agaagaaggg
     6661 caaggtagac accacgccga tctgcccggg tggcgccgca gtctggaccg ccaccacggt
     6721 ggtcacggtg atcatcccca tccccagacc cagaatcgcc ccgatctccg ccgcgtggtg
     6781 aagcggcgat ccggctccga ggcgcgtgaa caagaagtag ccggtcgtga ggctcagcat
     6841 cccggcaacg gccagggtgc gataacccag gcggttcaac agacgtccgc cgagggcgct
     6901 gcccacggtc cagctcaagc tggtgggggt caggacgctg ccggcctgaa tcgccgtgcc
     6961 gcccaacacc ccctggacga agagggggat gaaggagacg gccccgaaca tggcgacacc
     7021 cgacaggaag aggcaggccg tcgtcgtggt gaagattcgc cggccgaaga gtcccagggg
     7081 caggatgggc tccggcgccc ggctctcgat ccacaggaag acggcggtga acgtcaagaa
     7141 gatcgccagg cccgcgaggg cttcggcgcc aaagggacga cccacccggc cgacctccag
     7201 gagggacagc atcagggcga tgatgctgac cacaaggcag gcagtcccgg cgtagtccac
     7261 acgccggcgc gttccctcgc tagccaggcc cgtgagcccg atga
//
LOCUS       DBNGZC010000338         2855 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_28127, whole genome shotgun sequence.
ACCESSION   DBNGZC010000338 DBNGZC010000000
VERSION     DBNGZC010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_28127"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1323
                     /gene="rlmD"
                     /locus_tag="AC16G0_06140"
     CDS             <1..1323
                     /gene="rlmD"
                     /locus_tag="AC16G0_06140"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR5099240.1"
                     /translation="GKGQRIQVTIERLVFQGDGLGRLPDGRVIFVPYTAPGDLAEVEI
                     VEARSDFVRGRLGRLLTPSTDRAAPPCRYFGNCGGCQWQHLPHASQIHWKREVLLELL
                     ARVGTLDGIPAADPVTPAGPWEYRARAQLKVSGGNRPSIGFHRRETNRVVDIDRCPLL
                     DGRLNAVLAALRSMKHPSLSKLFPGLREVWMALGSGTGEAVVSLFAGVRERAAIRLLY
                     HTIQAALPSLQGVVLLEGDPRQHPRFVDRHGHGAIMEQVGEHRFRIDATAFFQVSGLA
                     AGLLASLVLELAALKGSERVLDLYCGVGTFTVPLSLRSREVVGVESSAEAAADGVFNL
                     RSNGCDRARIVHAQVEQVLPALLAEGRWDLIVLDPPRQGVSRRVLDTIAGEAVPRVIY
                     VSCDPSTLARDLGVFTRAGFRCVSLQPIDLFPQTFHLEVVGLLERPGV"
     gene            complement(1425..>2855)
                     /locus_tag="AC16G0_06145"
     CDS             complement(1425..>2855)
                     /locus_tag="AC16G0_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5099241.1"
                     /translation="VLGKAGRAETPTDPAPLSMLETVITLRPKAEWRTVDTWYSAWAP
                     EWAKPIFRRITPEHITQEELVRQMNEALQLPGVSNAWTMPVKARIDMLSTGVRTPVGL
                     KISGADLPTIEAIGAQIETLLPSVQGTRNVFAERTGAGYFLDVEWNREALARYGLGID
                     EAQTVVANAIGGETVTTTVEGRERYPVNVRYLRDFRSDLGALGRVLVAASGGQRQIPL
                     AQLATLKTVSGPAMIRNEDGLLTGYVYVDLAGRDPESYVAEAGRLLRDKVKLPPGYAI
                     AWSGQYEAMQRVAQKLWLVIPLTLCLIVLLLYMNTRSLGKTCLVALAVPFSAVGAVWF
                     LYLLGYHLSVGVWVGLIALLGVDAETGVFMLLYLDLAYEEAKREGRLRSLADLQQAIL
                     QGAVKRLRPKFMTVATMGLGLIPIMWSVGTGADVMKRIAAPLIGGILTSFLLELVVYP
                     PIYQLWKWHGEMKRQLSPEGGQAGIV"
BASE COUNT          445 a          940 c          983 g          487 t
ORIGIN      
        1 ggtaagggcc agcgtatcca ggtgacgatc gagcgactcg tattccaagg ggacggcctc
       61 ggcaggcttc cggatggtcg ggtcattttc gtgccctaca ccgcgccggg cgatctggcg
      121 gaggtcgaga tcgtggaggc ccggagcgac ttcgtccggg gtcgcctggg gcggctgctc
      181 acgccctcca ccgaccgcgc cgcgcctccg tgtcggtact tcggaaattg cggcggctgt
      241 cagtggcagc atcttcccca tgcctcgcag atccactgga agcgggaggt cctgctggag
      301 ctcctggcgc gggtcggaac gctggacggg attccggcgg cggatcccgt gaccccggcc
      361 ggcccctggg agtaccgtgc gcgggcgcag ctcaaggtgt cagggggaaa ccgcccctcc
      421 atcggtttcc accggcggga aacaaaccgc gtcgtggaca tcgaccgctg cccgctcttg
      481 gacggccgcc tgaatgccgt cctcgccgcc ctgcgtagca tgaagcaccc ctccttatcc
      541 aaactctttc caggcctgcg ggaggtgtgg atggccctcg ggagcggcac aggcgaggca
      601 gtggtctcgc tctttgcggg ggtccgggag cgcgcggcga tccggctgct gtatcacacg
      661 atccaggccg cactgcccag cctccagggg gtggtcctac tggaggggga tccgcgtcag
      721 catccccgct ttgtggatcg acacgggcac ggcgcgatca tggagcaggt aggtgagcat
      781 cgcttccgga tcgacgccac ggccttcttc caggtgagcg gtctggcggc agggcttctc
      841 gcatccttgg tgttggagct ggcggccctc aagggcagcg agcgggtgct ggacctgtac
      901 tgcggtgtgg ggacgttcac ggtgcccctg agcctccgca gtcgggaggt cgtgggggtc
      961 gaaagctctg ccgaggctgc cgccgacggc gtcttcaatc tccggagcaa cggctgcgac
     1021 agggcgcgga tcgtccacgc ccaggtggag caagtgctcc cggcgctcct cgccgagggg
     1081 cggtgggacc tcatcgtcct ggatcccccg cgccaaggtg taagccgtcg ggtcctcgac
     1141 accatcgcgg gtgaggcggt cccccgcgtg atctacgttt cctgcgaccc cagtaccctg
     1201 gcccgcgact tgggtgtctt cacccgcgct ggcttccggt gcgtgagcct ccagccgatc
     1261 gacctgttcc cccagacctt ccacctggag gtggtaggtc tcctcgaacg gccgggggtc
     1321 tgagcgtata gccgccggat caagtcgaga ggcccttggg ggcagagtct ttcagagccc
     1381 gcggagcggg cgaagcgcct cgccatcctc aggcaacggc cgcctcacac gatccctgcc
     1441 tgacccccct caggggaaag ctgccgcttc atctccccgt gccatttcca gagctggtaa
     1501 atcggggggt agaccaccaa ctccagcagg aaggaggtga ggatcccgcc gatcaagggg
     1561 gcggcgattc gcttcatgac atcggccccg gtcccgacgc tccacatgat ggggatgagg
     1621 cccaggccca tggtggccac tgtcatgaac tttggccgga ggcgcttcac cgcgccttgg
     1681 aggatcgcct gctggaggtc cgcgagactc cggagtcgtc cttcccgctt ggcctcctcg
     1741 tacgccaggt cgaggtagag gagcatgaag acgccggtct cggcgtccac tcccaggagc
     1801 gcgatgagcc ccacccacac cccgacgctc aggtggtagc ccaggaggta gaggaaccag
     1861 acggctccga ccgcagagaa gggcactgcc aaggccacga ggcaagtctt ccccagggac
     1921 cgggtgttca tgtagaggag gaggacgatg aggcagaggg tcagcgggat gacgagccag
     1981 agtttctgcg ccactcgctg catggcctcg tactggccac tccaggcaat cgcgtaccca
     2041 ggggggagtt tcaccttatc ccggagcagg cggcccgcct ccgcgacgta gctttcgggg
     2101 tctcgtccgg ccagatcgac gtaaacgtac ccggtgagca ggccgtcttc attccggatc
     2161 atggcggggc cgctcacggt cttgagggtg gcgagttggg ccagaggaat ctgccgctgt
     2221 ccgcccgagg cggcgaccag gacgcgaccc aacgccccca ggtcggagcg gaaatcccgc
     2281 aggtagcgca cgttcacggg atagcgctcg cgcccttcca cggtggtggt gacggtctcc
     2341 ccgccgatgg cgttggcaac gacggtctgg gcctcgtcaa tgccgaggcc gtatcgcgcc
     2401 aaggcctccc ggttccattc gacgtcgagg aagtagccgg cccccgtccg ctcggcgaag
     2461 acgtttcggg tcccctgcac cgaggggagg agcgtctcga tctgggcacc gatggcctcg
     2521 atggtcggaa ggtcggcgcc ggagatcttg agccccacgg gggtccgaac cccggtgctg
     2581 agcatgtcga tccgggcctt caccggcatg gtccaggcgt tggagacccc ggggagctgg
     2641 agcgcctcgt tcatctggcg gaccagctct tcctgggtaa tgtgctccgg cgtgatgcgt
     2701 cggaagatcg gtttggccca ttccggcgcc caggccgagt accaggtgtc caccgtgcgc
     2761 cactccgcct tgggccggag agtgatgaca gtctccagca tggagagagg cgcaggatcg
     2821 gtcggcgtct ctgctcgccc cgccttcccc aagac
//
LOCUS       DBNGZC010000339         2285 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1591542, whole genome shotgun sequence.
ACCESSION   DBNGZC010000339 DBNGZC010000000
VERSION     DBNGZC010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1591542"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..496)
                     /locus_tag="AC16G0_06150"
     CDS             complement(128..496)
                     /locus_tag="AC16G0_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018442161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099242.1"
                     /translation="MRKLLVIALAGCMLLSAVPSVALAGGPHAVRNRWAGVGIGAAVV
                     TLGGLLLGGLQAPVVAAPPPVVYTPPPVVYAPPPVVYAPPPVVYATPPPVVYARPPVV
                     VYRGWGTPGHWRHYRHWEDD"
     gene            complement(1140..1574)
                     /locus_tag="AC16G0_06155"
     CDS             complement(1140..1574)
                     /locus_tag="AC16G0_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009540569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099243.1"
                     /translation="MKRTLGATGVWLTVTFLVGGCVSLGERVLDSGTPTLQLRSFQTR
                     VFDTDDRERTLRTVIATLQDLGFLIEKADATLGAVSGTKVAQTGLWGLRMTVTVLPRG
                     QGRLLVRANAQLGQSAVTEAEPYQRFFAALEHAMFLTAHQVD"
     gene            complement(1571..2113)
                     /locus_tag="AC16G0_06160"
     CDS             complement(1571..2113)
                     /locus_tag="AC16G0_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099244.1"
                     /translation="MLKPQALSVRIVMTLVLLGGCAPSASTLLTLPEDSLAQRQLQTR
                     HFETNAEERVLAACTAVLQDMGFQIDETSPRLGVLLASKVRDTNLLRPEARLAATVLS
                     LGILSGLAVDQPAKIEVGIVTRRVGQAEERVSVRAVFRKTPLPPNGPRGMSQTITQPE
                     IYTQFFDSLSKALFLEARES"
BASE COUNT          433 a          750 c          665 g          437 t
ORIGIN      
        1 gtacctttct ttatcgttgc gtggccgatc tgggcggcga aaactttctg ctcctcgtta
       61 ctgagcctcg cacagaggac aggggcctct agaattccgc cgctgccaca tcagcacagc
      121 tgcaacccta atcgtcctcc cagtgccgat aatgccgcca gtggccggga gttccccatc
      181 cccgatagac cacgacgggg ggtcgagcgt atacaaccgg cggaggagta gcgtacacga
      241 ccggtggcgg ggcatacacg accggtggcg gggcgtacac gacgggcgga ggtgtgtaga
      301 ccacaggggg tggagccgcc acaacgggag cctgaaggcc gcctaggagc aggcccccta
      361 gggtgacaac ggctgcgccg atcccgactc ctgcccagcg attccggacg gcgtgtgggc
      421 ccccggccaa tgcgacagac ggcaccgcac tgaggagcat gcatccggcc aacgcaatca
      481 ccagcaattt ccgcatttta atccctcctg tccttccggt ctgttcccaa gtacggaatt
      541 gatggcaaga ctgtttctaa gttagtgctt actactgtag catcgcccat gccgtccaga
      601 atataatttc atacatccta aagattgctt tattttcaaa tacttacaat gaagaaaaat
      661 tccactgtcc gggtcttcag cattccagac ctgccctatt cggggtaggc catccacgat
      721 ctgggatatt tttgattatc gaaccatccg aattgctctt gacacattgt tggtcgccag
      781 acctgccgtc cgcaaagact actaccgccc cggataggcc ggcggatctt cacctaacct
      841 gctacacaca gggcagatgt tcctctttcc aacccaacac tcctgcccag cccggaccat
      901 gcgcacaacg ttggtgccgc tcccccggca gcctgaatgt gtaatggctg tcttggtccc
      961 caagatgccc tccggctccg gggcacgcga tatcgtccca tcctcgctta acgtggtgtt
     1021 ttcggtcatc gcccagattg cctccctacc tccgcccgtt cctccattgc gcattctttc
     1081 ggtcaggctc tccagacgcc tgccaggtcg ctgaccagca atgcgaatcg cccgatgttt
     1141 caatcgacct ggtgggccgt caggaacatg gcgtgctcta gggcggcgaa gaagcgttga
     1201 tacggctccg cctctgtcac cgcggactgg ccgagttgcg cgttggcccg gaccagcaag
     1261 cggccttgcc cgcgcggcaa tacggtgacc gtcatgcgga ggccccacag gccggtctga
     1321 gcaaccttgg tcccgctcac tgcccccagt gtggcatccg ccttctcgat cagaaacccc
     1381 agatcctgga gcgtggcgat gacggtgcgg agggtccgct cccggtcgtc ggtatcgaag
     1441 acgcgggtct ggaagctccg gagttgtaac gttggcgttc cggaatccag gactcgctcg
     1501 cccagggaga cgcacccccc gaccaggaaa gtgacagtga gccagacacc ggtggcgccc
     1561 agtgtgcgtt tcatgattcc cgagcctcca agaaaagcgc cttcgaaagg ctgtcgaaga
     1621 actgggtgta gatctctggc tgggtgatgg tctgggacat gccgcgcggt ccattcggcg
     1681 ggaggggcgt cttccggaag acggcccgga cgctgactcg ctcttctgcc tgcccgacgc
     1741 gccgcgtgac gatgcccacc tcgatcttcg ccggctggtc gaccgccaac ccactcaaga
     1801 tcccgagcga gaggacggtt gccgccagcc gcgcctcggg tcggagcaag ttcgtgtctc
     1861 gcacctttga ggccagcagt acccccaagc gggggctcgt ctcgtcaatc tggaacccca
     1921 tgtcctgaag aacggccgtg caggccgcca ggacccgctc ctcggcgttg gtctcaaagt
     1981 gtctggtctg cagctggcgc tgcgccagcg agtcctcggg gagggtgagg agcgtggacg
     2041 cgctcggggc gcaccccccc aggagaacca gcgtcatcac gatcctgact gaaagagctt
     2101 gcggcttcag catgggagcc tccctcagaa ccgcgaggag tggtacgcat actcgcggac
     2161 ccgcccctca ccatcgaagt tcacgatcac agtgacggtc cgctggctga ccgtggtgac
     2221 gccgccctgc cgggcatagc ccatcaggac caagccggca tacccggact cgtccaggta
     2281 cacgc
//
LOCUS       DBNGZC010000340         1624 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1299158, whole genome shotgun sequence.
ACCESSION   DBNGZC010000340 DBNGZC010000000
VERSION     DBNGZC010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1299158"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="AC16G0_06165"
     CDS             <1..935
                     /locus_tag="AC16G0_06165"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099245.1"
                     /translation="GGRASGQAVEIASVAQRLKTPYVVDHPSADIITAKGFEWVFRNN
                     PTGSIYPQAFNKFIVDVPGAMPKSAAVVYDNTVFGKTIAGAAMNFLKSKNVPIVSDEA
                     YPVNSLDFKPVMTKVKATNPDYVLLVAVATTDAILLTRHAKEMGVTPRAFVGFGGGFG
                     VEDFAKELGPLAQNVFSSAAWSGNPNDAATKEYYTKFQTKYGIHPKEHEVEGYAVIYI
                     LADALKRTKLTGNVEADREALRQSLLKTDMTTVFGKVKFGNWTGPIGDKYTNQNIYSP
                     EHSVMAQWLGGKLLNVWPKSNAETDYVFPDTTKR"
     gene            998..>1624
                     /locus_tag="AC16G0_06170"
     CDS             998..>1624
                     /locus_tag="AC16G0_06170"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5099246.1"
                     /translation="MPVETFVQSVVSGALTGSLYAMIGVGLTVVFGVMRIINLAHGEF
                     VMLGMYGAFWSLTLWKLDPFVSILLWTPLMFLAGMLVYRFLLQAIIPGGQMNTLLYKA
                     GLTLLIANLALFVWTGDYRTIKIPYAILPMRPFGIAVPIPLAIAFGLALLITGSLYLL
                     LTRTDVGRAIRATSQSPEAAALMGVNVDRISMITFGLGSALAGSAGVLL"
BASE COUNT          296 a          536 c          489 g          303 t
ORIGIN      
        1 caggcggccg cgcctccggc caggcggtgg agattgcatc ggtggctcag cggctcaaga
       61 ccccgtacgt agtggatcac ccctcggccg acatcatcac ggccaagggc ttcgagtggg
      121 ttttccggaa caatcccacg ggctcgatct acccgcaagc cttcaacaag ttcatcgtcg
      181 atgtcccggg cgccatgccg aaatcggccg cggtcgtcta cgacaacaca gtgttcggca
      241 agaccatcgc tggcgccgcc atgaacttcc tcaagtcgaa gaacgttccc atcgtcagcg
      301 acgaggccta tccggtcaat agcctggatt tcaagcccgt catgaccaag gtgaaagcga
      361 cgaatccgga ctacgtgctg ctggtcgccg tggcaaccac cgacgccatc ctgctgacgc
      421 ggcatgccaa ggagatgggc gtgaccccgc gggccttcgt tgggttcggc ggcggattcg
      481 gggtggagga tttcgccaag gagctggggc ccctggccca gaacgtcttc tcctcggccg
      541 cctggtcggg gaatcccaac gacgctgcga ccaaggagta ctacacgaaa ttccagacca
      601 agtacggcat ccacccgaag gagcatgagg tcgagggcta cgcggtcatc tacatcctcg
      661 ccgacgccct gaagcgcaca aagctcaccg gaaacgtgga ggcggaccgc gaggcccttc
      721 ggcaatcgct gctcaagacc gatatgacga cggtcttcgg caaggtgaag ttcggcaact
      781 ggaccggtcc gatcggcgac aagtacacca accagaatat ttactcgccg gagcactcgg
      841 tgatggcgca gtggctgggc ggcaagctcc tgaacgtctg gccgaagtcc aacgccgaga
      901 ccgattacgt cttccccgat acgacaaagc ggtagcgcgt tctcgctctg cgtcaccctc
      961 tctctcctgc gggggagaga gggtgacctg aggtaccatg cccgtggaaa cctttgtcca
     1021 gagcgtggtc agcggggccc tgaccggttc gctctacgcc atgatcggcg tggggctcac
     1081 cgtggtcttc ggggtcatgc gcatcatcaa cctggcgcac ggcgagttcg tgatgctggg
     1141 gatgtacgga gccttctggt ccctgacgct ctggaaactc gaccccttcg tctccatcct
     1201 cctctggacc ccgctcatgt tcctggcggg gatgctggtg taccgattcc tgcttcaggc
     1261 catcatcccc ggcggccaga tgaacacact gctgtacaag gcgggactga cgctgctcat
     1321 cgccaatctg gccctgttcg tctggaccgg ggactaccgg acgatcaaga tcccttacgc
     1381 gatcctgccc atgcggccct tcgggatcgc ggtgccgatt cccctggcca tcgccttcgg
     1441 tctggccctg ctcatcaccg gcagtcttta cctgctcctc acccggaccg atgtgggccg
     1501 cgccatccgg gccaccagcc agagtcccga ggccgccgcc ctcatgggcg tcaatgtgga
     1561 ccggatctcc atgatcacct tcggcctggg gagcgccctg gctggatcgg caggtgtgct
     1621 cctg
//
LOCUS       DBNGZC010000341         2337 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1495037, whole genome shotgun sequence.
ACCESSION   DBNGZC010000341 DBNGZC010000000
VERSION     DBNGZC010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1495037"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1431)
                     /locus_tag="AC16G0_06175"
     CDS             complement(<1..1431)
                     /locus_tag="AC16G0_06175"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR5099247.1"
                     /translation="MHGYAGRLLRVDLGNGKSWVEPLDGFRARRYLGGRGMGARILLD
                     EVPRGCDPLGPENRLVFAMGPLAGTFAPGSGRFVVLAKSPATDGFGEAYTGGFVAHEL
                     KYAGYDGIVVQGQASEKVYLSIVNDRVDIRPATHLAGRGTLETEETIKGELGDPEARI
                     ISIGVAGENGVRYACVMNDTDRAAGRTGLGAVMGAKNLKAIAIRGTGRVTLADPKRFR
                     EFALADLAKIRKHPWLGDSPSSAGALGTAGGVEDLSAAGILPTRNWETGAWKHADRIS
                     GTAMKNTILIGHRACQACPVGCTRVVRVADGPFGGVRPEYGGPEYETVAAFGSLCENS
                     NLEAIALLNQRCNVYGLDTISAGSVIAFAMECFAKGILTEQQIGFRLDWGDAPAMVRL
                     LDMIARREGVGDLLAEGTRRAAAQLGRGASELAVEVKGLEFGMHEARGKKGLAISYAT
                     APRGADHMEGFHDPSLAVPNAWPELGV"
     gene            complement(1849..>2337)
                     /locus_tag="AC16G0_06180"
     CDS             complement(1849..>2337)
                     /locus_tag="AC16G0_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR5099248.1"
                     /translation="QHGEDVAVLAGKSVRGIVLGVVVTALIQVAVGGIGLFLAGVPAA
                     ALLTAVMSILCLAQLGPILVLIPALIWVYLSGQPVWGMVLLVFSVVVATIDNFIRPFL
                     IKKGADLPLLLIFAGVIGGLIAFGIIGLFVGPVVLAVFYTLLKAWVSGDVLEDEAGSG
                     TE"
BASE COUNT          415 a          839 c          701 g          382 t
ORIGIN      
        1 gaccccgagc tcgggccagg cattgggcac cgcgagcgac ggatcgtgga acccttccat
       61 gtggtccgcg ccgcggggcg ccgtggcgta cgagatggcg aggcctttct tgccgcgggc
      121 ctcgtgcatc ccgaactcca gccccttgac ctccaccgcc agctcggacg ccccgcgccc
      181 gagctgtgca gccgcccggc gcgtgccttc agccaggaga tcgccgaccc cctcgcgccg
      241 ggcgatcatg tccagcaagc ggaccattgc cggcgcgtct ccccagtcca accggaagcc
      301 gatctgctgc tcggtgagga ttcccttcgc aaaacactcc atggcaaagg cgatcacgga
      361 gcccgcgctg atcgtgtcca gcccgtacac gttgcagcgc tggttgagca gcgcgatggc
      421 ctcaaggttg gaattctcgc agagcgagcc gaaggccgcc accgtctcgt actcgggccc
      481 gccgtattcc ggccggaccc cgccgaatgg gccgtctgcg acccgcacga cgcgcgtgca
      541 gccgacaggg caggcctggc aggcccgatg gccgatgaga atcgtgttct tcatggccgt
      601 gccgctgatc cggtcggcgt gcttccaggc tccggtctcc cagttacgag tcggcaggat
      661 gccggcggcg gacagatcct cgacgccgcc cgcggtgccg agcgcacccg cactgctcgg
      721 gctgtcgcct aaccaagggt gcttccggat cttggccagg tcggcgaggg cgaactcccg
      781 gaacctcttt gggtcggcca acgtgacccg gccggtccca cgaatggcta tggccttgag
      841 gttcttcgcc cccatcaccg cgccaagccc tgtgcgcccc gccgcccggt cggtgtcatt
      901 catgacgcag gcgtagcgca ccccgttctc ccctgccacg ccgatgctga tgatccgggc
      961 ctcaggatcg cccaactccc ccttgatcgt ctcctcggtc tcgagcgtcc cccgccccgc
     1021 cagatgcgtg gctggccgga tgtccacccg gtcgttgacg atggagaggt agaccttttc
     1081 cgaggcctgg ccctgcacga cgatgccgtc gtagccggcg tacttcagtt cgtgcgccac
     1141 gaagccgccg gtgtaggcct caccgaagcc gtcggtggca ggggatttcg cgaggaccac
     1201 gaagcgtccc gagccgggcg cgaatgtgcc cgccagaggg cccatcgcga acaccagccg
     1261 gttctccggc cccagggggt cgcaccctct ggggacctcg tcgagcagga tccgcgcccc
     1321 catcccccgg ccgcccaggt accggcgcgc tctgaacccg tcgagcggct cgacccagct
     1381 tttcccattg ccaaggtcca ctctgagcag tcgtcctgcg tatccgtgca tgggatccgc
     1441 ctttcgcgat gagtgtggat aggtgtccga cggacattct atggcaggct tgctcaccat
     1501 caacctgaaa cgggcagttg tgttcaaagt cagtcaattg ccagggcgcg cccctggtgc
     1561 tgccctccgc gcagccgggt cggtcggcgc gcagcgaact cggccgaatt caccgcctgt
     1621 aacaggttca tggccttaag gcgcgtgtct ccgcgcgacc atcgccgcag ccaccgcgcc
     1681 accgtacgga gacgagacgc gcctcttgca tacggacgtc tcgtggctag ccaaggaata
     1741 ttcttctcgc cggtggaccg tgcgtcggaa tgcgcaccat caccccttcc tccgcaacca
     1801 agattgactc cctcgacgtc tatgggcccc tcggttcttc ggagtcgttc actcggtacc
     1861 tgagcccgcc tcatcctcca agacatcgcc cgacacccat gccttgagca gtgtatagaa
     1921 gaccgcaagc accacgggac caacgaagag gccgatgatg ccgaaggcga tgagacctcc
     1981 gatgacgccg gcgaagatca gcaggagcgg cagatcggcc cctttcttga tcaggaacgg
     2041 tcggataaag ttatcaatgg tcgcgacgac gacagaaaaa acgaggagca ccatccccca
     2101 gaccggctgg ccggacaagt agacccagat taacgcgggt atcagcacca gaatcggtcc
     2161 gagctgcgcc agacagagaa tcgacatcac ggccgtcaac aatgccgcag caggaacacc
     2221 ggcgaggaac agcccgatac cccccacggc tacctgaatg agcgcagtca caacgactcc
     2281 cagcacgatg ccccgcacgg acttccccgc cagaacggcc acatcctctc catgctg
//
LOCUS       DBNGZC010000342         3763 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1397526, whole genome shotgun sequence.
ACCESSION   DBNGZC010000342 DBNGZC010000000
VERSION     DBNGZC010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1397526"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..1174)
                     /locus_tag="AC16G0_06185"
     CDS             complement(236..1174)
                     /locus_tag="AC16G0_06185"
                     /inference="COORDINATES: protein motif:HMM:NF014003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM hydrolase/SAM-dependent halogenase family
                     protein"
                     /protein_id="HIR5099249.1"
                     /translation="MKNQKKSSASEGESASGGVPMARHSEGRARSADHRPIVSLLTDF
                     GHQDPFVGIMKGVILSLCPEAILVDLCHETPAFNILGGSFLLQSAVPFFPAGTIHVAV
                     VDPGVGGPRRPILARIGQHVFVAPDNGLLSYPMAAGTVHSVRAITASKYLLHPVSATF
                     HGRDVFAPVAGHLAWGVPPEQLGPEISDAVRLAMPHPQRDTSGALRGVVLWVDRFGNC
                     ITNLTQEELGKLAEVGGQRVQIRINDRPLGPLVGSFGEAGEGGRGAIIGSTGHLELFS
                     NRGNLASERALRLGAPICVEWERESDAGRVFRKKRG"
     gene            1214..1300
                     /locus_tag="AC16G0_06190"
     tRNA            1214..1300
                     /locus_tag="AC16G0_06190"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1248..1250,aa:Leu,seq:cag)
     gene            complement(1383..2792)
                     /locus_tag="AC16G0_06195"
     CDS             complement(1383..2792)
                     /locus_tag="AC16G0_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR5099250.1"
                     /translation="MPSLVLVVEDETILGESISVYLERHGLSTALARSGEEAIKLAEE
                     SSPDVAVMDVRLPGINGLEVLRRMRELSPGTEVIMMTAHASVASAVEAMKLGAFDYLT
                     KPLDLDELRVVVDKALSHQRMRRELSYLKAREETGGHLAEIVGESQSIRTLKQRIERI
                     ASLESSDGSSAPTVLIVGETGAGKEMVARAIHYGSARAKGPFVEINCAAIPASLLEAE
                     LFGYEKGAYTDARTAKPGLFEAADGGTLFLDEIGHMDIAIQVKLLKAIEEKAVRRVGG
                     LRTKSFNARIIAATNRDMEAAIAEGAFRSDLYYRIKALTIEVPPLRERGTDIVLLARH
                     FLERFTRQYGLPPRALSPEAETLLQAYAWPGNVRELAHVMERAVLLHATPRVQPEHLG
                     LSGSREKASVVVGAGGGVHVDFAVGGIVLDEVERQLITEALQSVSWNRTKAAQLLGIS
                     KETLRYRMEKYQLQGPTAG"
     gene            complement(2796..3497)
                     /locus_tag="AC16G0_06200"
     CDS             complement(2796..3497)
                     /locus_tag="AC16G0_06200"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5099251.1"
                     /translation="MEAERLAAIGEVVTAVAHGIRNPLANIRAAAQVASLESRDAGPS
                     TLVPKSLVNIMLEVDRLEGRLKELLQFVRPANRPSEPLDLNTVLRESLRMVEGRIAKT
                     HLRVEEQLAEGLPPLMGSAILLEQVFLSLIGNAIEAIPDGAGTISILSGRDGNGTQPP
                     SIFVEVRDTGVGIPAEQLSRIFEPFYTTKAQGTGLGLAIAKKFAEAHGGTLTVRSSPG
                     EGTAFRVTFPALLEA"
     gene            complement(3539..>3763)
                     /locus_tag="AC16G0_06205"
     CDS             complement(3539..>3763)
                     /locus_tag="AC16G0_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HIR5099252.1"
                     /translation="VPIVFPGTSRVVGVVETYKMPGQVFASIRKGQLTVIVTALMGGA
                     FLYLSLFWIVRRAGRRIESSTRRWSNGRRS"
BASE COUNT          664 a         1253 c         1153 g          693 t
ORIGIN      
        1 cttaacaaca cgcttggtga cctttcaagt caaatttatc tttatccggg cctcagcact
       61 atctggctgg ctgccggaag aaccttccat cggccaggct gctcacatca gatcaagacg
      121 acgcaacggt tcttcgagcg ggacctgagc gacaacccat ggcacgggag cagcgcaatc
      181 gaaggccgtc ggccgccggg taccccccgc caggttgggt atggcccggg catgatcatc
      241 ctcttttctt ccgaaaaacg cggcctgcat ccgactccct ctcccactcc acacatatcg
      301 gcgcgccgag cctgagggcc cgctcgctgg caaggtttcc tcgattcgag aagagttcca
      361 ggtgacccgt gctgccgatg atggcccccc gaccgccctc accggcctcg ccgaaagaac
      421 caaccagtgg acccagcggc cggtcattga tgcgaatctg cacccgttgt cccccgactt
      481 ccgccagctt ccccaactct tcttgcgtga ggttcgtgat gcagttcccg aagcgatcca
      541 cccacagaac cacaccgcgg agggcgccag aggtatcccg ctggggatgg ggcatcgcca
      601 ggcgcacggc gtcagagatc tcagggccaa gctgttccgg tgggacgccc cacgccagat
      661 gcccagccac gggggcgaat acgtccctcc cgtgaaacgt cgcactgacc ggatgcagca
      721 ggtatttgct ggccgtgatc gcccgcacgc tgtggactgt ccctgcggcc atgggatagc
      781 tgagcagccc gttgtcgggc gccacgaaga catgctggcc gatccgggca aggatcggcc
      841 gccgtggccc accgacccct gggtccacca cggccacgtg gattgtcccc gcgggaaaga
      901 acgggactgc cgattggagg aggaagctcc cgcccaggat attgaaggcg ggcgtctcat
      961 ggcagagatc caccaggatc gcctcgggac acaggctgag gatcacgccc ttcatgattc
     1021 ctacgaaggg gtcctgatgc ccgaagtccg tcagcaggct gacgatggga cgatggtccg
     1081 ccgagcgcgc tctgccttca ctgtgtcgtg ccataggaac gccgccagac gccgattctc
     1141 cctccgacgc cgagcttttc ttctgatttt tcattgactt tttcaactga agaggctaca
     1201 agaagggcca cctgccgaag tggcggaact ggtagacgcg ctacgttcag ggcgtagtgg
     1261 gggaatcccc atgggggttc gagtcccccc ttcggcacca tgtatgtgta gcagaaggcc
     1321 gtgacgggaa tgtcacggct ttttgctttc cgttccccat caccccctgt cccgctacac
     1381 cctcatccgg ccgtgggacc ttggagttgg tacttctcca tgcggtatcg gagggtctcc
     1441 ttggagatac cgagcaactg cgccgccttg gtccggttcc agcttaccga ttgaagcgcc
     1501 tcggtgatga gttgacgctc cacttcgtcc aggacgattc cgcccacggc gaaatccacg
     1561 tgcacgccgc cgccggcccc cacgacgacg gatgcctttt ccctgctgcc cgagaggccg
     1621 agatgctccg gctgcactct cggcgttgcg tgcaagagca ccgcccgttc catcacgtgg
     1681 gccaactcac ggacgttccc cggccaggca tacgcctgca agagcgtctc cgcctcagga
     1741 ctgagcgccc tcggagggag gccatactga cgggtgaacc gctccaagaa gtgccgggcc
     1801 aagaggacga tgtccgtccc ccgctcgcgg agggggggca cttcgatggt cagggccttg
     1861 atgcgataat ataggtcgga gcggaaggcc ccctcggcaa tggcggcctc catgtcgcga
     1921 ttggttgccg cgatgatgcg ggcgttgaag gattttgtcc gcaggcctcc cacccggcgc
     1981 accgccttct cctcgatcgc cttcaggagc ttgacctgga tggcgatgtc catatgcccg
     2041 atctcatcga gaaacagggt cccgccatcc gcggcctcga acagaccggg cttggcggtt
     2101 cgcgcatccg tgtacgcccc tttctcgtac ccgaacagtt cggcctccag aagcgaggca
     2161 gggatggcag cgcaattgat ctcaacgaac gggcccttgg ctcgggcgct gccgtaatgg
     2221 atggcgcggg ccaccatctc cttccccgcc ccggtctcgc ccacgatcaa gaccgtgggg
     2281 gcgctgctcc cgtccgagga ttccaggctg gcgattcgct cgatccgttg cttgagggtc
     2341 cggatggact gagactctcc aacgatctcc gccaggtgcc cgcccgtctc ctcccgggcc
     2401 ttcaggtagg acaactctcg ccgcatgcgc tggtgagaca gcgccttgtc taccaccacc
     2461 cgaagctcat ccagatccag cggcttcgtc aggtagtcga aggccccgag cttcatggcc
     2521 tcaaccgccg aggccacgga ggcgtgggcc gtcatcatga tgacttcggt tccgggggaa
     2581 agctcccgca tccgacgcag gacttccagc ccgttgatcc cgggaagccg cacatccatg
     2641 acggccacat cggggctcga ctcctcggcc agcttgatcg cctcttctcc ggagcgcgcc
     2701 agagccgtgg agagcccgtg ccgttccaga tagacagaga tcgattcccc caggatcgtc
     2761 tcatcttcaa ccacgagcac taacgatggc atggttcagg cctccaggag ggcggggaac
     2821 gtcacgcgga aggcggtacc ctccccgggc gagctacgga cggtgagggt tccgccgtgt
     2881 gcctcggcga acttcttggc aatggccaaa cccaggccgg tgccctgtgc cttggtcgtg
     2941 tagaagggct cgaagatccg ggagagctgt tcggcgggga tgccgacgcc cgtatcccgt
     3001 acctcgacaa aaatcgaggg aggctgcgtc ccgttgccgt ccctgccgga gaggatggag
     3061 atggttcccg cgccatctgg aatggcctcg atggcattcc cgatcagact caggaacacc
     3121 tgctccaaca ggatggcgct tcccatcagc gggggcaggc cctcggcgag ttgctcctcc
     3181 accctgaggt gggtcttggc gatgcgtcct tccaccatcc gcagggattc acgtagcacg
     3241 gtgttcagat ccaggggctc gcttggccgg ttggctggtc gaacgaactg caagagctcc
     3301 ttcaggcggc cctcgagtcg gtccacctcg agcatgatgt tgacgaggct tttcggcacg
     3361 agcgtggacg gtccggcgtc tcgggactcc aggctggcca cctgagccgc ggcgcggata
     3421 ttggcgaggg ggttccggat cccgtgcgcc accgccgtga ccacctcgcc tatggctgcc
     3481 agccgttcag cctccaacag ttgcgcctgg acggcgcgca gctcctcatt ggcagatatc
     3541 agctcctgcg tccgttgctc cagcgccgtg tgctgctctc gatgcggcgc ccggcccggc
     3601 ggacgatcca gaagagcgac aggtagagga aggccccgcc catcaaggcg gtgacgatga
     3661 cggtgagttg tcctttccga atgctggcga agacctgtcc gggcatcttg tacgtctcga
     3721 ccacgccgac gacccgcgag gtgccgggga aaactatggg cac
//
LOCUS       DBNGZC010000343         1072 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_614118, whole genome shotgun sequence.
ACCESSION   DBNGZC010000343 DBNGZC010000000
VERSION     DBNGZC010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_614118"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="AC16G0_06210"
     CDS             <1..682
                     /locus_tag="AC16G0_06210"
                     /inference="COORDINATES: protein motif:HMM:NF027651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HIR5099253.1"
                     /translation="VGTIIVYILGVHEDAALAAFLFAFFVIATVSFEFVISTRTRARS
                     TGEGLLEAFFALLLRSRRRYGGLIVHLGVVIAIIGIAVSSIYKVEREATLKPGETLSI
                     GHYSVRFDSLAAGERPTHILVWANLMGLKDGKPIGELTPGERFYPNQQTPFASVDARY
                     HWKEDLYVILSAFERDGSSATIKVLINPMISWIWIGGAVIVLGVLVAVLPERRLALLS
                     VRARAQEA"
     gene            685..>1072
                     /locus_tag="AC16G0_06215"
     CDS             685..>1072
                     /locus_tag="AC16G0_06215"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR5099254.1"
                     /translation="MAAGTRFISWKKALIPLASLPLIALLAYGFRVDPKAIPSPLVKK
                     PAPAFQLSIYDGEPMSLEGLRGKPVVLNFWASWCFPACYEEAPLLESTWREYKQKGVV
                     FLGVDIQDKESNAREFLRRFNYTFPNG"
BASE COUNT          175 a          346 c          345 g          206 t
ORIGIN      
        1 cgtcggcacg atcatcgtgt acatcctggg cgtgcacgag gatgcggccc tggcggcgtt
       61 cctgtttgcg ttctttgtga ttgccacggt gagcttcgag ttcgtgatct cgacgcgaac
      121 ccgggcccgg tccaccggcg aggggctcct ggaggcattc ttcgccctcc tgctgagaag
      181 ccggcgacgc tacggaggcc tcatcgtgca cctgggcgtg gtgatcgcca tcatcgggat
      241 tgccgtctct tcgatctaca aggtcgagcg cgaggccacc ctgaagccgg gtgaaacctt
      301 gagcatcggg cactacagcg tgcgctttga cagtctggcg gcgggagagc ggccgaccca
      361 tatcctggtc tgggccaacc tcatggggct caaggacggc aagcccatag gcgagctgac
      421 gccgggggag cgcttctacc cgaaccagca gacacccttc gccagcgtgg atgcgcgcta
      481 ccactggaag gaggatctgt acgtgatcct ctctgccttc gagcgcgatg gcagctccgc
      541 caccatcaag gtgctgatca accccatgat ctcctggatc tggatcgggg gcgccgtgat
      601 cgtcctgggc gtgctggtgg cggtcctccc ggagcggaga ctcgccctcc tgagcgttcg
      661 ggcccgggcc caggaggcgt gaccatggct gccggcacac gcttcatctc ctggaaaaag
      721 gcgctgattc ccctggccag cctgcccctg atcgcgctcc tggcctacgg atttcgggtc
      781 gatcccaagg ccattccatc cccgctggtg aagaaaccgg cccccgcctt ccagctgtcg
      841 atctacgacg gggagccgat gtccctggag ggactcaggg ggaagcccgt ggtcctgaac
      901 ttctgggcct cctggtgttt cccggcctgc tatgaggagg cgccgctcct ggaatcgacc
      961 tggcgggagt acaagcagaa gggggtcgtc ttcctgggcg tggatatcca ggacaaagag
     1021 tcgaatgccc gcgagttcct gcggcgcttc aactatacct tccccaacgg cc
//
LOCUS       DBNGZC010000344         2292 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1690711, whole genome shotgun sequence.
ACCESSION   DBNGZC010000344 DBNGZC010000000
VERSION     DBNGZC010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1690711"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            461..1786
                     /locus_tag="AC16G0_06220"
     CDS             461..1786
                     /locus_tag="AC16G0_06220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HIR5099255.1"
                     /translation="MRIVVKTNPYDDFRLHWDFARRFVAGQNLYGGGDTPYPPFWGMA
                     NAPMTVVPPRIAHIVLLPLGAAALVVLTSALHSVSRRHLPLDRDRLFWATAAALALSS
                     RFLIRDLPECGPNIAMVALSWAGLALWRNGRDRAGGACLGLAMALKCTPALFVPYLAL
                     KRPWRMTAWAVATSAILTLAPLAWQGPTSFARHFRQWAGNCWNGLGGLDPSIGVLGQE
                     EVWNVSLRPTIARLLMHLPPGHKGRIDSPWRAQVFDLSPPVAGALIKSTLFLLLAGMA
                     WTFRHSARDRDDGAVPWEYAAVSLLILLSSPLTWKQHCVGAYPACYLIARAMVARWAL
                     PRWAIGALCGYVVLVLLLDRGVVGRSMTLLLDSWGVITWSLLLLLAVTLACRSRQVAA
                     IREASRGGAVPPPHFAEARTPWLLRGAFLRWSWITGRQSSRCPWGATTR"
     gene            1765..>2292
                     /locus_tag="AC16G0_06225"
     CDS             1765..>2292
                     /locus_tag="AC16G0_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099256.1"
                     /translation="MGSDHPLIRRVGIVRTLTPPFSGNATRCSDEPRSERWLDRLTWA
                     FVGFGVLLRLVRYLLRHPLWGDEAFLAVNFLTRDYINLLRPLDYGQVSPLLFLWIERA
                     LIDWLGFSEFSLRLFPTACSVASVLLFRLVAGRVVRGAPLLLAVAIFSVSFYPIRHGG
                     EVKAYASDLLVGMLLL"
BASE COUNT          324 a          722 c          759 g          487 t
ORIGIN      
        1 tcaaagttca ttacaagcct caccgatatt ccctcgaggg actggagata ggtcgcccgg
       61 gggtctgtgc ataccgatcc ctgcactgcc cgggtcatgg ttctgggctg aatcggtcga
      121 ccctccggtc aatgtaggcc cagtaccaga gacgctgtca ccgagtgcca tttggccttc
      181 gctgtcggag actttcccga aaagggctca tctcgggaaa gtcctggacc ggtgttggtg
      241 agaaattccc tcgactgaag tgtcaagcag ccaataagct atgcccgttg gcacctcata
      301 gcaccgtctc tgggattggg ccgggccatg gaggcggttg cgagacgagg acagaacgat
      361 ggaccaacga gggatcgtgg gaccttcgac acacggacgc gcgcggagag tgaggctggc
      421 cgtcacgatt gtcgtgctcg gagtcctgat ctcgcactgg gtgcgaattg tcgttaagac
      481 gaacccgtat gatgacttcc gactccactg ggatttcgcc cgacggttcg tggctggcca
      541 gaatctctat ggtggagggg atacccccta tcctccgttc tggggaatgg cgaacgcacc
      601 gatgaccgtc gtgccgccac ggatcgccca tattgtgctg ctcccgctgg gggcggcagc
      661 actggtggta ttgacgtccg cactccactc cgtgtcccgg cgtcatttgc ctctggacag
      721 agaccggctg ttctgggcga cggccgccgc cctagccctc tccagccgat tcctgattcg
      781 cgacctgccc gagtgcgggc cgaatatcgc gatggtggcg ctcagttggg ccgggctggc
      841 cctctggcgg aacggccgcg atcgggctgg aggggcctgc ctggggctgg cgatggcgct
      901 gaagtgtact cccgccctgt ttgtccccta cctcgccctg aagcggccat ggcggatgac
      961 cgcatgggct gttgcgacct cggcgatcct cacgctggcc ccgctggcgt ggcaggggcc
     1021 tacgtcattc gcgcggcatt ttcgacagtg ggcgggcaac tgctggaatg gcctgggggg
     1081 gcttgacccc tcgatcgggg tgctgggtca ggaagaagtt tggaacgtct cactgcgccc
     1141 gacgatcgcg cggttgctga tgcacctgcc gccggggcac aagggccgaa tcgattcccc
     1201 ctggcgggcc caggtcttcg acctgtcgcc tcccgtcgcg ggggccctca tcaagagtac
     1261 cctgttcctg ctgctggccg ggatggcatg gaccttccgc cactcggctc gggaccgcga
     1321 tgacggcgct gtgccctggg agtacgcagc cgtctccctg ttgatcttgc tctcttcgcc
     1381 gctgacctgg aagcagcact gcgtcggtgc ttatccggcg tgctatctga tcgccagagc
     1441 gatggttgcg cggtgggcct tgcctcggtg ggccatcggg gccctctgcg gctacgtggt
     1501 tctggtcctc ctcctcgacc gcggagtggt cggtcggtca atgacgctcc tgctcgatag
     1561 ctggggcgtg atcacgtggt cgctcttgct cctgttggct gtgacgttgg cctgccggtc
     1621 ccgccaggtg gcagcgatca gggaggccag tcggggtggt gccgtcccgc cgccccactt
     1681 cgccgaagcg cggacgccct ggctcctacg tggtgctttc ctgcgatgga gttggatcac
     1741 cgggcgtcag tcctcgaggt gcccatgggg agcgaccacc cgctgatcag gcgtgtcggg
     1801 attgtcagga cgctcacacc ccccttttcg gggaacgcga cgcgatgcag tgatgagccg
     1861 cgatcggaac gctggctgga caggctgacg tgggccttcg tcggcttcgg cgtgctcttg
     1921 aggctggtac ggtacctgct gcggcatccg ctctggggcg acgaggcctt cctggccgtc
     1981 aacttcctca cacgagacta catcaacctg ctgcggccgc tcgactacgg ccaagtcagc
     2041 ccgctcctat tcctgtggat cgagcgggca ctgatcgact ggctcggctt cagcgagttt
     2101 tcgttgcgac ttttccccac ggcctgcagc gtggccagcg tcttgttgtt tcggctcgtg
     2161 gccggccgtg tcgttcgcgg agcgccgctc ttgctggccg tggccatctt ctcggtgtcg
     2221 ttctacccca tccggcacgg cggggaggtc aaagcctatg cttcggatct cctggtcggg
     2281 atgctgctcc tg
//
LOCUS       DBNGZC010000345         4895 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_516535, whole genome shotgun sequence.
ACCESSION   DBNGZC010000345 DBNGZC010000000
VERSION     DBNGZC010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_516535"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="AC16G0_06230"
     CDS             complement(<1..308)
                     /locus_tag="AC16G0_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099257.1"
                     /translation="MSRKTSGMPVRNSSATNTSVAASGRVTRRRFLRDSALALAAAGS
                     AGYGAFASAQTKGDGPVLEFKFAEIQPAQGIYARHEHRFAKLVEERSQGKVKVAVFAG
                     G"
     gene            complement(371..1666)
                     /locus_tag="AC16G0_06235"
     CDS             complement(371..1666)
                     /locus_tag="AC16G0_06235"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR5099258.1"
                     /translation="MSLFLLFAVMLAFMAVGMNIGLAMVASSLVYLVSHANNPMPIPL
                     AVMPMKMLYGVDSFPLLAVPLFMLAGELMSAGGITTRIVRFATALVGWITGGLAHVNI
                     VVNMIMAGMSGSAVADAAATGSILIPAMVMVGFSASFAAAVTAAAATIGPIIPPSIPF
                     VLIGSIAQVSIGRLFLGGAVPGVIMGLFLMATSYWVAKRQGYAVEQRAGLGELLSSLW
                     SSAPALLMPLIVMGGILSGVTTPTEAAVVAVAYALVLAVGVYRQLTLVDCYRICVRMG
                     SSTAAIMLTIAGATLLGFVATAEQMGPKLMRVFLAFSQDPMIILLVINVAFLILGWVM
                     EPVPIILLTVPIFFPALTKIGVDPIHLGVVMTLNLMIGLLTPFVGLNVFITSAIARVP
                     VITVARESLIFIATLVIVLLLITLFPQLVVWLPNQVMGR"
     gene            complement(1663..2154)
                     /locus_tag="AC16G0_06240"
     CDS             complement(1663..2154)
                     /locus_tag="AC16G0_06240"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR5099259.1"
                     /translation="MSVKRYTDVVFEAVTAFLLMVMVALTLLQVITRYGLHAGFEWTE
                     ELARLDLIYLTFFGSIVAFQRREHLRIETLVYALPSRARRRLRVAVDLVSMVVLGVVV
                     WQGVPLLKRFWPLLSAALNWPTTFFYFPVVFGCFVMLIYAGLDVIGALRGAEDRPREK
                     EAP"
     gene            2481..3398
                     /locus_tag="AC16G0_06245"
     CDS             2481..3398
                     /locus_tag="AC16G0_06245"
                     /inference="COORDINATES: protein motif:HMM:NF026954.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR5099260.1"
                     /translation="MSLLIRRSTLILPVNNPRFVEKAYRRGADAIMLDLEDAVPLKVK
                     DAARLLIRDSIPLVGRGGAEVFVRVNNDPAMLALDLEASVQPGLDGICYPKTESAEEV
                     QHLDAQISDLERRRGIAPGRIELFLLIESPRGLLNLEAIAAGGPRVRSMTLGPEDYCL
                     TLGVEPSADGMELLYGVSKVVTVCKATGISPMGLLGSIAGFRDLAAFERAAARARDLG
                     CVGASCIHPDQVAVLNRVFSPPTEKVKYAQAAVEAFEEGLKRGTASVNVAGTMVDIPV
                     YRRAKRILEQAAAVALVEQRKTDALARSG"
     gene            3424..4683
                     /locus_tag="AC16G0_06250"
     CDS             3424..4683
                     /locus_tag="AC16G0_06250"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR5099261.1"
                     /translation="MDGTDLRDFIRERRIGLAQSRLPLRDIRIIDLGSVVAGPFGATL
                     LGDYGAEVVKIEPPGVPDALRYWAVIEARHEPFWLLASRNKLPITLNLKHPQGREILA
                     RLVAQSDVLLENMRPGTLDRLGFPAAKLWEINRGLIIGRISGYGQTGPYASKPGFGTL
                     AEAMSGFTYMNAQPGAPPTNPPLALADMIAGTHLALGVIIALRDQKRGERGGQELDLS
                     LYEPLFGFMGGDFLMHALSGIAPHPVGNESNYVSPRNSYRTADGRWVALSASSQAPFE
                     RLMDLVGRPDLKQTPGFRNNQERLQQASRDVLNRIIGDWIKARPLAEVLEACEKAGVT
                     IGPVYSMEDIAHDPHYRARGSITSIFDPASQKEIPFPETPIRLSATPGAIRFPGLPMG
                     AANEVVLEDLLGYSPEEVADFRRAGAI"
BASE COUNT          906 a         1559 c         1647 g          783 t
ORIGIN      
        1 ccgcccgcga agaccgcaac cttcaccttc ccctggctgc gttcctccac cagcttggcg
       61 aacctgtgct catggcgcgc gtagatgccc tgagcgggct ggatttcggc gaacttgaac
      121 tcgaggaccg ggccgtctcc cttggtttgg gccgatgcaa atgcgccgta gccggccgac
      181 cctgcggcgg cgagggcgag cgccgagtcc cgcagaaacc tgcgtcgggt caccctgccc
      241 gaggcggcga ccgatgtgtt ggttgcgcta ctgttgcgga cgggcatccc tgatgtcttc
      301 cggctcatgc tggatctcct ttctcgctgc atctatcccg gtcgagagcg tctcgcggga
      361 ccaaaggggt ctacctgccc atgacctggt tgggcagcca gacgaccagt tgggggaaca
      421 gggtgatcag gagcagcacg atcacgagcg tggcgatgaa gatgagggat tcgcgggcaa
      481 ccgtgatgac cgggacccgc gcgatggccg aggtgatgaa cacgttgagg cccacgaagg
      541 gtgtgagcag cccgatcatg aggttcaggg tcatgacgac cccgaggtgg atcgggtcca
      601 cgccgatctt ggtcaacgcg gggaagaaga tcgggacggt gagcaggatg atcgggaccg
      661 gctccatcac ccaccccagg atcaggaacg ccacgttgat gaccaggagg atgatcatgg
      721 ggtcctggct gaaggccagg aagacgcgca tcagcttggg gcccatctgc tccgccgtcg
      781 ccacgaagcc gaggagggtg gctccggcaa tcgtcagcat gatggcggcg gtggagctgc
      841 ccatgcgcac gcagatgcgg tagcagtcca ccagcgtgag ctggcggtag acccccacgg
      901 ccaggacgag ggcataggcc accgccacga ccgccgcttc cgtgggggtc gtgacgccgc
      961 tcaggatgcc gcccatgacg atgaggggca tcaacaatgc gggcgccgac gaccagaggc
     1021 ttgaaagcag ctcgcccagg ccggcgcgct gctcgaccgc atagccctgc cgcttggcta
     1081 cccagtagga ggtggccatg aggaagaggc ccatgatgac tcccggcacg gcgcctccca
     1141 ggaacagccg gccgatggag acttgtgcga tgctcccgat caggacgaag gggatgctgg
     1201 gtgggatgat cggcccgatg gtcgccgccg ccgccgtgac cgcggcggcg aaggacgccg
     1261 agaagccgac catgaccatg gctgggatca ggatggatcc cgtggccgcc gcatccgcga
     1321 ccgcggaccc ggacatgcca gccatgatca tgttgacgac gatgttgaca tgcgccagcc
     1381 ccccggtgat ccacccgacg agggctgtgg cgaagcgcac gatccgcgtc gtgatgccac
     1441 ccgccgacat caactctccg gccagcatga agagcggcac ggcgaggagg gggaaggagt
     1501 ctacgccata caacatcttc atcggcatga ccgcgagtgg aatcggcatg gggttgttgg
     1561 cgtgtgacac cagatacacc aggctgctgg cgaccatcgc cagaccgatg ttcattccca
     1621 cagccatgaa ggccagcatc acggcgaaca ggaggaacag gctcatggcg cctctttctc
     1681 acggggccgg tcctcggctc cacggagcgc tccgatcacg tcgagcccgg cgtagatcag
     1741 catcacgaag cagccgaaga cgaccgggaa gtagaagaag gtcgtcggcc agttcagggc
     1801 ggcggagagg agcggccaga atctcttcag gagcggcaca ccctgccaga ccaccacgcc
     1861 gagaaccacc atggagacca ggtccacggc cacccggagc cggcggcgcg cccgcgatgg
     1921 cagcgcataa accagcgtct cgatgcggag gtgctcgcgc ctttggaagg ccacgatcga
     1981 gccgaagaag gtcaggtaga tgagatcgag ccgcgccagt tcctcggtcc actcaaagcc
     2041 ggcgtggagc ccgtagcggg tgatcacctg gagcagcgtg agggccacca tgaccatgag
     2101 caggaatgcg gtcaccgcct caaacacgac atcggtgtat cttttgacgg acaccgaacc
     2161 cccccaagac tggacggcgg gaggaatcaa gagtattgcg gtcgccgcgg aaactaccat
     2221 cgagtaccag gggtgtcaag aaacatgggg ggaaggtatg gagaagatgg taagcagggc
     2281 aggagtaatc agccggtcac ggtaggtcgt ggcggaagcg aggcacacag gagaggtcag
     2341 ggtctggcgt gatcgacggg gcaggctgtc accacatttt cccttgactt ccctccatcc
     2401 tcgcggctag ggtctgtcgg ttttccagag cgtcgttgcc ccgcttcggg cgggccgggt
     2461 ggcatccaag aggtgaacgc atgagtctgc tcatcaggcg gtccacgctc atcctgccgg
     2521 tgaacaaccc gcgattcgtc gagaaggcgt atcgccgcgg ggccgacgcc atcatgctgg
     2581 acctggaaga cgcggtcccc ttgaaggtaa aggacgccgc gcggctgctg atccgcgact
     2641 ccatcccgct cgtaggccgc gggggcgcgg aggtctttgt ccgggtgaac aacgaccccg
     2701 ccatgctggc cctggatctg gaggcgtcgg tccaaccggg gctcgacgga atctgctatc
     2761 ccaagacgga gtcagccgag gaagtccagc acctggatgc ccagatctcc gatctggagc
     2821 ggcgccgcgg gatcgctccc ggtcggatcg agcttttcct gctcatcgag agccctcgcg
     2881 gtctgctgaa cctggaggcc atcgccgccg gcggtccaag ggtccggtcc atgaccctgg
     2941 gccccgagga ctattgcctg acgctcgggg tggagccctc ggcagacggg atggaattgc
     3001 tttacggcgt gtcgaaagtc gtgacagtct gcaaggcgac agggatcagc cccatgggcc
     3061 tcttgggcag catcgccggc ttccgggatc tagcggcgtt cgaacgggcc gccgcccggg
     3121 ccagggatct cgggtgcgtg ggggcttcct gcatccatcc ggaccaggtg gccgtcctga
     3181 accgcgtctt ctctccgccg acggaaaagg tgaagtacgc ccaggcggct gtggaggcgt
     3241 tcgaggaggg gctgaagcgg ggcacggcct cggtaaacgt tgccggcacg atggtggaca
     3301 tcccggtcta tcgacgggcg aagcgtatcc tggagcaagc cgcggccgtc gccctggtcg
     3361 agcagcggaa gacggatgcg ctggctcgat ccgggtgaag gcgggcaggg gtgaggagcg
     3421 ctcatggacg gaaccgacct cagggatttc atccgggaac gacggatcgg gctggcgcag
     3481 tcgcgcctcc cgctgcgtga catccgtatc atcgacttgg gctcggtggt ggccggcccc
     3541 ttcggcgcca cgctcctcgg agattacggc gccgaggtcg tcaagatcga gcctccaggt
     3601 gtccccgatg ccctccgtta ctgggccgtg atcgaggcgc ggcacgagcc cttctggctc
     3661 ctcgcctcgc ggaacaagct gcccatcacg ctgaacctga agcatccgca gggccgggag
     3721 atcttggcca ggctcgtcgc ccagtccgac gtcctcctgg agaacatgcg gcccgggacg
     3781 ctcgaccgcc tgggctttcc ggccgccaag ctctgggaga tcaaccgggg gctgatcatc
     3841 ggacgcatct cgggctacgg ccagaccggc ccgtacgcct ccaagccggg cttcggcacg
     3901 ctggccgagg ccatgagcgg attcacctac atgaacgccc aaccgggtgc gccacccacc
     3961 aaccctccgc tcgccctggc ggacatgatc gccggcaccc acctcgccct gggcgtgatt
     4021 attgctctcc gtgatcagaa gcgcggggag aggggcggac aggagctgga cctctcgctc
     4081 tacgagccgc tcttcgggtt catggggggc gacttcttga tgcacgccct gtcgggcatc
     4141 gcgccccatc ccgtgggcaa cgaatcgaac tacgtttccc cgcggaatag ctaccggacc
     4201 gcggacggaa gatgggtggc cctctccgcc agctcccagg cccccttcga gcgcttgatg
     4261 gacctcgtcg gccgtcccga cctcaagcag acaccgggct tccgaaacaa ccaggagcgg
     4321 cttcagcagg ccagccgcga cgtcctgaac cggatcatcg gcgactggat caaggcgcgg
     4381 cccctcgcgg aggtgctgga ggcttgcgag aaggcgggcg tgaccatcgg tcccgtctac
     4441 agcatggagg acatcgcgca cgatccccat taccgggcca ggggcagcat cacctcgatc
     4501 ttcgatcccg catcgcaaaa ggaaattccc ttccccgaaa cccccatccg gctgtccgcc
     4561 acccccggcg cgatccgttt tccgggcctc cccatgggcg cggccaacga ggtcgtcctg
     4621 gaggacctgc tgggctacag ccccgaggaa gtcgcagact tcaggcgcgc cggcgcaatc
     4681 tagacgtctc gaatcggggc tgcgttgccg tcgactcatt cggcaggatc tgggaagcta
     4741 tggccgcgat gtgcggtgga gggctcatcc caagggctgg gtggctctgg agggtggttg
     4801 acctcgtctc ggcattcccg gtctgtgaat gattcgggcc tgctactcca tgagcttcac
     4861 gaagagggag ccatccaaga ctttgatgtc ttcca
//
LOCUS       DBNGZC010000346         2830 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1592044, whole genome shotgun sequence.
ACCESSION   DBNGZC010000346 DBNGZC010000000
VERSION     DBNGZC010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1592044"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            362..1510
                     /locus_tag="AC16G0_06255"
     CDS             362..1510
                     /locus_tag="AC16G0_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR5099262.1"
                     /translation="MSPLSHRERVLAVLNHEVPDRFPVDLGGGPASGINLHAYKRLKE
                     HLGLPGPIRVQTERSLLAWPDEGLLERFDVDLRLVVARTAEDSGERGIFKEAAYATEA
                     EYTDVWGVVRRRPPHGHYYVVHAPFEKDDLCLADLDAHPWPSPTTTDVTGLTREALRL
                     RRETDCALVVHVPGRIFSLGQFMCGFANWLVQLKLNREFCEALLDRALDIQLAMAEAT
                     LQAVGDAADILYLADDDGTQTGPLISPDLFRQIFKPRMARLIRFLRERSSARIAFHSC
                     GSIYAFIPDFIDAGVQILNPVQVSAAEMDTARLRREFGRHLVFWGGVDSQRVLPQGTP
                     NDVRAEVARRVQDLGPAYIPMSVHNIQAEVPPENILAMFEAIHDARPV"
     gene            1533..2762
                     /locus_tag="AC16G0_06260"
     CDS             1533..2762
                     /locus_tag="AC16G0_06260"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5099263.1"
                     /translation="MQDFTRIARKITWVLFLSQGLSSAGFIAAFTVNALVAVDLTGQT
                     AMAGVPGALYVLGQACGAMLWGCSMERLGRRRGLAFGQILGVIGSAIAMAAVVNRYFL
                     FFLIGLIFVGMARSAVDLGRFVAAEVHLPSQRGRAISNVVLGSTVGAIVGPLLVGPTG
                     RMAAASGFPELAGPYAVGFAGLVLAALLIFGGLRPDPRDIGQELARRYPESVPHQTSR
                     SLTEIMGQPGVVVATGTMVFAQLVMVVPMSITSVHMIAHQHPLTAVSLVISAHTLGMY
                     AFSVISGRMTDRWGRGPVIILGSALLILSCLLAAPSTTLLPLVTALYLLGLGWNFAYV
                     AGSTLLADQLSPKERAKTQGFNDLLLNLASAASQVVSGVVYAIGGFGVMGLTAAGAAA
                     VPLCLGIWWQLTVAHKQ"
BASE COUNT          443 a          962 c          876 g          549 t
ORIGIN      
        1 cgtcttctcg aatgtcatcc cgactcgcaa ttgatcactc atgtcttcct cgctccggtc
       61 tctataggtt caatgcctcc accgtccata cggaattccc caaagcgctt tctcagcaca
      121 gaccttcggg cgggcacgtc cctccccacg cagagacgtg ccggctacaa tatcagagtc
      181 cccgccgtcc cgtccatgcc cctgccatgc cgatttctgg gaagcctgcc gagggcctct
      241 ccggctaacc cgggccccgt acctccacga tcgaggcgca cgccatgagg attgaccgat
      301 cccgtcccga gtcttatagt gcaccagcca tgcacacgga catctgacag gagaagccag
      361 catgagtcca ctctcacacc gcgaacgggt cttggccgtt ctcaaccacg aggtgccgga
      421 tcgctttccg gtagacctgg gcggagggcc ggccagcggc atcaatctcc acgcgtacaa
      481 acgactgaaa gagcatctcg gcctgcccgg cccgatccgc gtgcagaccg agcggtcgct
      541 cctggcctgg ccggacgagg gcctcctgga gcggttcgac gtggacttgc gtctggtcgt
      601 ggctcgcacg gcagaggatt caggggagcg cgggatcttc aaggaggccg cctacgccac
      661 cgaggcggag tacaccgacg tgtggggcgt cgtgcgccgg cggcctccgc atggtcacta
      721 ctacgtcgtc cacgccccgt tcgagaagga cgacctctgc ctcgcggacc tggatgcgca
      781 tccttggccc tcgcccacaa caacggacgt gacggggctc acgcgcgagg ccctgcgcct
      841 tcgccgtgag acggactgcg ccctggtggt ccacgtgccc gggcggatct tctccctcgg
      901 ccagttcatg tgcggctttg ccaactggct ggtccagctc aagctgaacc gcgagttctg
      961 cgaggcgctg ctcgaccggg cgctggacat ccagctcgcc atggccgaag cgacgctcca
     1021 ggccgtgggg gacgccgcgg atattctcta cctggccgac gatgacggga cacagaccgg
     1081 gcccctcatt tctcccgacc ttttccggca aatcttcaaa ccccgaatgg cccgcctcat
     1141 ccggttcctg cgcgagcgct cgtccgcccg gatcgccttt cacagttgcg gttcgatcta
     1201 tgccttcatc ccggacttca tcgacgcggg ggtgcagatc ctgaatccgg tccaggtctc
     1261 cgccgccgag atggacaccg cgcgtttgcg gagagagttc ggccgccacc tcgtcttctg
     1321 gggtggcgtg gacagccagc gggtcctgcc acaggggact cccaacgacg tccgagcgga
     1381 ggtcgcccgc cgtgtgcagg atctcggccc cgcttacatc cccatgtccg tccacaacat
     1441 ccaggccgag gtgcccccag agaacatcct ggccatgttc gaggccatcc acgacgcccg
     1501 ccccgtgtaa ccgcttcgcc tgagggagat gaatgcagga cttcactcgc atcgcccgga
     1561 agatcacgtg ggtcctgttc ttgtctcagg gcctcagctc tgccgggttc atcgccgcct
     1621 tcaccgtcaa cgccctggta gccgtggatc tcaccggaca gacggcgatg gccggtgttc
     1681 ccggcgctct ctacgtgctg gggcaggcat gcggcgccat gctgtgggga tgcagcatgg
     1741 agcggctggg ccgccgccgg ggtctcgcct tcggccagat tcttggggtg atcggatcgg
     1801 ccatcgccat ggccgccgtg gtcaaccggt actttctctt tttcctgatc ggcctcatct
     1861 ttgtgggaat ggcgagatcg gccgtggacc tgggtcgctt tgtcgcggcg gaggtccacc
     1921 tgccatcgca gcgcggacgg gccatctcca acgtggtcct ggggagcacc gttggcgcca
     1981 tagttggacc cttgctggta ggccccacgg ggcgaatggc ggcagcatcc ggattcccgg
     2041 agctggcggg accttacgcc gttggattcg ccgggctggt cctggcggcc ctgctgatct
     2101 tcgggggact gcggcccgat ccccgcgaca tcgggcagga actcgcccgc cgctacccgg
     2161 aatctgtccc ccaccagacg agcaggtccc tgacggagat catgggccag ccgggggtgg
     2221 tcgtggccac gggcaccatg gtttttgccc aactggtgat ggtcgtgcct atgtccatca
     2281 cgtcggtgca catgattgcc caccagcatc cgttgaccgc cgtgtcgctg gtgatctccg
     2341 cccacactct cgggatgtac gccttttccg tcatctcggg aagaatgacc gaccggtggg
     2401 ggcgaggacc cgtgatcatc ctgggatccg cgctgttgat cctctcctgt ctgttggccg
     2461 cgccttccac aactctgtta cctctggtga cggcgctcta cctcctaggc ctgggctgga
     2521 actttgcgta tgtggctggt tctaccctgc tggccgacca gctctcaccc aaggagagag
     2581 ccaagactca gggtttcaac gacctgctgc tgaaccttgc ttcggcggcc agccaagtcg
     2641 tgagcggggt ggtctatgct atcggcggct tcggggtcat ggggctgaca gccgcgggag
     2701 ctgccgccgt gccgctctgc ctgggcatct ggtggcaact cacggtggcc cataagcaat
     2761 aggccaggaa ggcgctcgtt tgatcggccc attgaccgca caaagggtgc cggtgcgtgc
     2821 gaacacactt
//
LOCUS       DBNGZC010000347         1995 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1202270, whole genome shotgun sequence.
ACCESSION   DBNGZC010000347 DBNGZC010000000
VERSION     DBNGZC010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1202270"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="AC16G0_06265"
     CDS             complement(<1..252)
                     /locus_tag="AC16G0_06265"
                     /inference="COORDINATES: protein motif:HMM:NF015125.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu C-terminal domain-related protein"
                     /protein_id="HIR5099264.1"
                     /translation="MWSISWTARSGQAPWGQRPWRPAWVPWRSARQTAASDRETESSW
                     SCVPRPSVSVLVRFTLIRPVGIEPGMRFALREGGRTIGAG"
     gene            complement(533..964)
                     /locus_tag="AC16G0_06270"
                     /pseudo
     CDS             complement(533..964)
                     /locus_tag="AC16G0_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(983..>1995)
                     /locus_tag="AC16G0_06275"
     CDS             complement(983..>1995)
                     /locus_tag="AC16G0_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099265.1"
                     /translation="ADFGNPALLGGNLSFLATDAFLMITGEWNTEMASVISVVLLVTS
                     LAIFAAQYTRLRGKVFTTVGGAPVAAEERGVSSWLLGVLLAPCLLYAAVVAVSYGVIA
                     LGAVTKLVGIDNTFTLEHLVKNVGLEAIWTSVKVSLLAGGFAALGGTGLAYLCMRGDI
                     WLRGLLEMITLVGFALPGTVMGIGYLLAFNRPPLLLTGTLAILVLNMIARFLAVGLEA
                     GVSKLYQIDRSLEEASADLGAGTLTTFRRIVLPLMSSACVAGFLFTFMQSMISLSAAI
                     FLVAPGRLLASVYIFNRARDGDMGIACAISVILIASVAFCLVGLAFLARRTGVRIFAA
                     QA"
BASE COUNT          383 a          638 c          678 g          296 t
ORIGIN      
        1 gccggcgccg atggtgcggc cgccttcgcg gagcgcgaac cgcatgcccg gctcgatccc
       61 caccggacgg atcaaggtga accgtacaag gaccgagacg gatggcctcg ggacgcacga
      121 ccaggaagac tctgtctccc ggtccgaagc ggccgtttgt cgagcggatc gccaaggcac
      181 ccaggcgggt cgccacggtc gttggcccca aggcgcctgc ccggagcgag ccgtccagga
      241 aattgaccac accgatgaag cgagccacga actccgtcgc ggggcgctcg tagatctcgg
      301 tgggggtccc ggtctggagg atgcgcccct ggttcatcac catgatccgg tcggagatgc
      361 tcatggcctc ctcctggtca tgggtgacga agaccaccgt ttgctgcagg tcccgctgaa
      421 ggcgcgtgat ctcggcccgc atctgcaccc ggagattggc atccaaattg gacagcggtt
      481 cgtccagcag gaggaccttg ggttggaggc tgagggcccg cgccagggcg acccgctgtt
      541 gctggccgcc ggagagctct atcgggcggc gaccgctgac accctgaagc tgcaccagga
      601 cgagcaattc ctcgacgcgt cgggtgatct ccgcccgggg gcgcttccgg atcgccaggg
      661 cgtagccgat gttctgagcc acggtcagat gggggaacag ggcatagttc tggaagacca
      721 tggcggttgg ccgatgattc ggcgggagcg cggtgatgtt ctcgccgtcg aggacgatgc
      781 acccggcgtc agggcggaga aagccgccga tcagccgcaa cagggtcgtc ttgccgcacc
      841 cgctcggccc gacgaaagac accagctccc ctcggtggat ggcggcgctg acgttgtcca
      901 cggccgtcag ggcgccgtat cgcttggtga ggccgtcgag actcagcagc gggcgatcgg
      961 tcatcgttgc tccagggtcg cctcaagcct gggcggcaaa gatccggacg cccgtccgcc
     1021 tggcgaggaa ggccagaccc accaggcaga acgcgacgct ggcgatgagg atgacggaga
     1081 tggcgcaggc gattcccatg tctccatcgc gtgcccggtt gaagatgtag accgaggcca
     1141 gcagccgtcc gggtgccacc aggaagatag cagcgctgag gctgatcatg gactgcatga
     1201 aggtgaagag aaagccggcg acgcaggcgg aagacatcag gggcaagacg atccggcgga
     1261 aggtggtcag ggtcccggcg cccagatccg ccgacgcctc ttccaggctc cgatcgattt
     1321 ggtagagctt gctcaccccc gcctccagcc ctaccgccag gaagcgggcg atcatgttca
     1381 ggaccaggat cgccagtgtt ccggtgagga ggagcggggg acggttgaag gccagcaggt
     1441 agccgatgcc catgaccgtc ccgggcaggg cgaagccgac cagggtgatc atctccagga
     1501 gaccccgcag ccagatgtcg ccccgcatgc acaagtaggc aagacccgtc cccccgaggg
     1561 cggcaaatcc ccccgcaagg agtgacacct tcacgctggt ccatatcgcc tccaggccca
     1621 cattcttgac caggtgttcc agggtgaagg tgttatcgat gcctaccagc ttcgtcactg
     1681 cgccgagggc gatgaccccg taggagactg ccacgaccgc cgcgtacagg aggcaaggag
     1741 cgaggaggac ccccaagagc caggagctga ccccccgctc ctctgcggcg accggggccc
     1801 cgcccaccgt ggtgaaaacc tttccgcgca accgggtgta ctgcgccgca aagatcgcaa
     1861 gactggtaac caagaggact acggagatca cgctggccat ctccgtgttc cactcgccgg
     1921 tgatcatcag gaaggcatcc gtggcgagaa acgacaggtt gcctcccaag agggccgggt
     1981 tgccgaagtc ggcga
//
LOCUS       DBNGZC010000348         2633 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2082898, whole genome shotgun sequence.
ACCESSION   DBNGZC010000348 DBNGZC010000000
VERSION     DBNGZC010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2082898"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="AC16G0_06280"
     CDS             <1..409
                     /locus_tag="AC16G0_06280"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR5099266.1"
                     /translation="EVPGSADALRFLTRVPEGIKLAVVTGSRRLEVESILWRLAISER
                     FQALVTAEDYAQAKPAPEPFLTAARRLDLLPMSCLVIEDSPAGVVAALAAGMPVVAVD
                     RRPGESELGQATWKVSSLDDLTLTDKGEVVVKG"
     gene            559..1431
                     /locus_tag="AC16G0_06285"
     CDS             559..1431
                     /locus_tag="AC16G0_06285"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HIR5099267.1"
                     /translation="MKIQSPYRKLPQWFRGCTHTHSTLSDGEWPLEQVVAFYRDRGYA
                     FLAMTDHDVFTDLSRFSTPDFLALSSSEVTVRGREHIVGLRLKGQVESFTDHQSTINA
                     INDQSGLAILAHPNWASFSLERCLGLHGYTAIEIYNVVCNYLEYNGYALQYWDELLRR
                     GGRVWGVAADDAHRIPYQGAKAWIVVDAERLAAEEILKSIRSGNFYASSGPHFQGFEV
                     DGNTIKVWCSPAVEIRFMTGDLNPGLRLRGDGLASAAYTPRPEDAYVRIEVVDAQMQS
                     AWSQPFFIYPDWRP"
     gene            complement(1473..1838)
                     /locus_tag="AC16G0_06290"
     CDS             complement(1473..1838)
                     /locus_tag="AC16G0_06290"
                     /inference="COORDINATES: protein motif:HMM:NF017355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HIR5099268.1"
                     /translation="MSKPETCILRIPTDLKQRLEQEAEREGLSLNQWALYNLSQSVAF
                     TEAYRELRSRLSRIDMLKAKETVWRLLTRPTPEEPRPAWDAAPRGWKEYEPELRLDIP
                     PEQPAARKAGRPKTRRAPR"
     gene            complement(2073..2306)
                     /locus_tag="AC16G0_06295"
     CDS             complement(2073..2306)
                     /locus_tag="AC16G0_06295"
                     /inference="COORDINATES: protein motif:HMM:NF021560.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HIR5099269.1"
                     /translation="MTSLQYYFDKEADVLYVSQGEPRSDVDSEEVGEGVVARLDPSTH
                     EVIGFTILNFLKRADKGLPAVVLPFRAELTLVP"
     gene            complement(2303..2608)
                     /locus_tag="AC16G0_06300"
     CDS             complement(2303..2608)
                     /locus_tag="AC16G0_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099270.1"
                     /translation="MSTRRFTSVLATDIELTDERREHIALFHQDLAPFLDRLGDVLAY
                     PDSLRRSLDDPQVILFYKHFPDILGGKYVTVVVKTAPERSFVLTAYLTRSIRTGVAL"
BASE COUNT          480 a          786 c          861 g          506 t
ORIGIN      
        1 cgaggtcccc gggtcggcgg acgccctgcg attcctcact cgtgttccgg aagggatcaa
       61 gctcgccgtg gttaccggtt cccggcggct cgaggtcgag tcgatcctgt ggagattggc
      121 gatatccgag cgctttcaag ctctggtcac ggccgaagat tacgcccagg ccaagcccgc
      181 accggagccc ttcctcacgg cggcgcgccg gttggacctc ttgccgatgt cctgtctggt
      241 gatcgaggac agcccggccg gcgtcgtggc cgcgctggcc gccgggatgc cggtggtggc
      301 ggtggaccgg cgtccagggg agagcgagct gggtcaggcc acgtggaaag tctccagtct
      361 ggatgacctg accctgacgg acaagggaga agtcgtggtg aagggatagc tgacagagat
      421 cagcgaccag cgatcagagg tcagagaccc gagaccagag gtcagagatc ggcggtctgg
      481 ggaggatccg aggggattcg ctgggttacg ggttcccgaa ggctgagggc ttggggcggc
      541 ttggacagga gagcacccat gaaaatccag agtccgtatc gaaagctgcc gcagtggttc
      601 cgtggctgca cgcacacgca ctcgaccctc tcggacggcg agtggccgct ggagcaggtg
      661 gtggcctttt atcgcgatcg gggatatgcc tttctggcca tgaccgacca cgacgtcttc
      721 accgatctca gccggttctc gacgccggat ttcctggcct tgtcgtcaag tgaagtgacg
      781 gtgcgcggcc gggaacacat cgtgggtctc cggctcaagg ggcaggtgga gtccttcacc
      841 gaccaccagt caacgatcaa cgccatcaac gatcagagcg gcctggctat cctggcccat
      901 cccaactggg cctccttcag cctcgaacgg tgtctgggcc tgcacggata cactgccatc
      961 gagatctaca acgtcgtctg caattacctc gagtacaacg gctatgcgct gcagtactgg
     1021 gatgaactct tgcggcgagg ggggcgggtg tggggggtcg cggccgacga cgcccaccgc
     1081 atcccctatc agggggccaa ggcctggatc gtcgtggacg ccgagcgctt ggccgccgag
     1141 gagatcctga agagcatccg gtcggggaac ttctatgcct catcaggccc ccacttccag
     1201 gggttcgagg tggatggaaa tacgatcaag gtctggtgct cgccagccgt cgagatccgg
     1261 ttcatgaccg gtgacctcaa tcccggacta cggctgcggg gagacggcct cgcgtcggcg
     1321 gcgtacacgc ctcggcccga ggatgcctat gtccggatcg aggtggtgga tgctcagatg
     1381 cagagcgcct ggagccagcc cttcttcatc tacccggact ggcggccgtg acgtttcttg
     1441 aagagctgac agtccgccgt ccgaatgtcg ccctatctcg gtgcccggcg ggtcttggga
     1501 cggcccgcct tcctggctgc tggctgttct ggcgggatgt ccaggcggag ttctggttcg
     1561 tactccttcc agccgcgagg agcggcgtcc cacgccgggc gaggttcctc cggggtgggg
     1621 cgggtgagca gccgccagac ggtctccttg gctttcagca tgtcgatccg actcaggcga
     1681 gatcggagtt cccgatatgc ctcggtgaag gcgaccgact gcgagagatt gtagagcgcc
     1741 cattggttca gcgagagccc ttcccgctca gcctcctgct cgagcctctg cttcagatcg
     1801 gtcggtatcc gtagaatgca ggtttcaggc ttgctcatgg tgctgcctcc aaatcctcag
     1861 aaactgacca ggggtgacga cgtcggctgg caactggaat tctgcccgcc tagccccctg
     1921 tagatcacct cctgcacccc gtcctgtccg tacttattcg tctcccccgg cacctgcatc
     1981 cttccgggag cacggtccat gaagtgggcg atgggcgcca ggtgctggga agagctacct
     2041 gtcggcaggc aaggcgctac ggtcgaagtc ggctacggca ccagcgtcag ttccgcacga
     2101 aatggcagga ccaccgcggg caggcccttg tctgctcgct tgagaaaatt caggatggtg
     2161 aagccgatga cctcgtgggt gcttgggtcc aagcgggcaa cgactccttc cccgacctct
     2221 tcggagtcga catcagatcg cggctccccc tgagacacgt agaggacatc ggcctcctta
     2281 tcgaaatagt actgtaggct ggtcatagcg caactcctgt tcggatgctt ctcgtcaggt
     2341 atgccgtgag tacgaaagac cgttctgggg cggtcttcac cacgacagta acatacttgc
     2401 cgcccaggat atcggggaaa tgcttgtaga agaggatcac ctgtggatca tccaggcttc
     2461 tccggagtga atcggggtaa gccaggacat cgccaagccg atccagaaat ggggcgagat
     2521 cctggtggaa cagcgcaata tgctcccggc gctcatccgt tagctcaata tcagtggcga
     2581 ggacactggt gaagcgccga gtggacaccg caggagggta tcaaatagga gtc
//
LOCUS       DBNGZC010000349         2181 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_711929, whole genome shotgun sequence.
ACCESSION   DBNGZC010000349 DBNGZC010000000
VERSION     DBNGZC010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_711929"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="AC16G0_06305"
     CDS             complement(<1..181)
                     /locus_tag="AC16G0_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIR5099271.1"
                     /translation="MDETTTFIRERLQKLEDLSNDGIDPYPSTFPVTHQAGDLKARYD
                     AADDATLSAAERVGLG"
     gene            587..1162
                     /locus_tag="AC16G0_06310"
     CDS             587..1162
                     /locus_tag="AC16G0_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099272.1"
                     /translation="MHIRLHDLVGILLMTGIISLVGTEPLPAANPPPGSKTASVRLLD
                     LTAQVPATWVAQQPSSSMRLAQFRVPGKTGSEDAELVVFHFGQGQGGSAEANIARWQS
                     EFSSPDGKPVKASIHRLKVSGMPATTVELTGSYARTLGMTAAGTPIPDQTLLAAIVES
                     PKGNLFIQLHGPKATVAANREAFLAMIRGMK"
     gene            complement(1275..>2181)
                     /locus_tag="AC16G0_06315"
     CDS             complement(1275..>2181)
                     /locus_tag="AC16G0_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007721835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR5099273.1"
                     /translation="VELGRLTRTLGGIILAKLEHLNPGFSKKDRIARQIIEDAEAQGM
                     LQAGQTVVELTSGNTGTGLAIVCGIKGYPFVAVMSRGNSAERARMMTALGAEVVLVDQ
                     MPGSRPGQVSGEDLKLVEEATQRLVEARRAFRADQFRLAGNFRAHYLHTGPEILRQAR
                     GRIDAFCDFVGTGGTYAGCAAAFKEHDPAIHCYVVEPAGAAILAGEPVTHPGHRIQGG
                     GYAMPELPLLRPEHIDGYLQVSDREAMEWARRLAREEGIFAGFSSGANVAAAVQLLQG
                     PCQGKTIVVTICDSGLKYLSTDLWP"
BASE COUNT          373 a          736 c          679 g          393 t
ORIGIN      
        1 cccccagacc taccctctcg gccgccgaca gcgtggcatc gtccgcggcg tcgtagcgcg
       61 ccttgagatc gcctgcctga tgcgtgaccg ggaaggtgct cgggtaagga tcgatgccgt
      121 cgttggagag atcttccagc ttctggagcc gctcccggat gaaggttgtc gtctcgtcca
      181 ctgcttctcc gtttatcccg aaagtttcac cgtcaaggcc gcaaagttcg cagaggcgcg
      241 ccgccactgg gcgctttgcg gttccatcga agcatgcggg tgtcttggca aaagtgccgc
      301 gattataggg agggctccgg agggtgtcaa gctgcggcgg acccatcgcc tccctcggcc
      361 aatggtagta gacgcgcaga ccgcgccgcc aactggctcc atcctatcga catccgcatt
      421 tggtcattgg tcattgggat ttggtcattg cctcaagcta ctcgcgaacg ggttcgagaa
      481 ttatctggct taaggtgata ccctcaatga gggaggccac ggaggcaagg tttccgactt
      541 aggctttcgc acggtccaaa tggcatccac agcgcgggag ggcgcgatgc atatcaggct
      601 gcacgacctc gtggggatcc tgctgatgac gggcataata tcactggttg gaaccgaacc
      661 gcttcctgcc gcgaatccac cgcccggctc caagaccgcg agcgtccggc tactcgacct
      721 gaccgctcag gtccccgcaa cctgggtggc tcagcaaccg tccagctcca tgcgtctcgc
      781 ccagttccgg gtgccgggaa agaccggctc cgaggacgcg gagcttgtcg tgttccactt
      841 cggccagggg cagggcgggt cggccgaggc gaacatcgcc cgctggcaat cggagttctc
      901 ctcccccgac ggcaagccgg tgaaggccag catccaccgt ctcaaggtca gtggcatgcc
      961 ggcgaccact gtcgagctga ctgggtcgta cgcgcgcacc ctgggcatga cggccgccgg
     1021 cacccccatt ccggaccaga ccctgctggc cgccatcgtc gagtcgccga agggcaatct
     1081 cttcatccag ctccacggcc ccaaggccac cgtggccgcc aaccgcgagg cattcctagc
     1141 gatgatccgg ggaatgaagt agcctgaata cgcgggacgg cctgcgcctc gtgcgctgtg
     1201 caccatgctc acgctggaat tcttaaccct ggccccaggc ggagccagcc ggagcatcgg
     1261 atctttgttg tacgtcaggg ccagaggtcg gtgctgaggt attttaggcc ggaatcgcag
     1321 atggtgacga cgatcgtctt cccctggcag ggtccctgca gcaactggac agccgcagcc
     1381 acgttcgccc ccgaggagaa tccggcaaag atgccttcct ctcgcgccag ccgcctcgcc
     1441 cactccatcg cctcgcgatc ggacacctgg aggtacccat cgatgtgctc ggggcggagc
     1501 aacggaagct ccggcatggc gtagcccccg ccctggatgc ggtgcccggg atgggtgacc
     1561 ggctccccgg cgaggatggc cgcccctgcc ggctcgacca cgtagcagtg gatggctgga
     1621 tcgtgctctt tgaaggcggc cgcgcacccc gcgtaggtcc cgcccgtccc gacaaaatcg
     1681 cagaaggcgt cgatcctgcc tcgcgcctgc ctcaggatct cgggaccggt gtggaggtaa
     1741 tgggcgcgga agtttcccgc cagtcggaac tggtcggcgc gaaaggcgcg gcgcgcttcc
     1801 accaggcgct gggtggcttc ttccactagc ttcagatcct cgcccgagac ctgcccgggc
     1861 ctggagcccg gcatctggtc cacgagcacg acctccgctc ccaaggccgt catcatccgg
     1921 gcgcgctcgg cggagttccc ccgcgacatg acggccacga agggataccc cttgatcccg
     1981 cacacgatcg ccagaccggt gcccgtattg ccggaagtca actccacgac ggtctggccg
     2041 gcctggagca tcccctgagc ctcggcatcc tcgatgatct gacgggcaat tctgtctttc
     2101 ttggaaaacc ccgggttgag atgctccagc ttggccagta tgattccgcc gagcgtccgg
     2161 gtgagtcgcc ccagctcgac c
//
LOCUS       DBNGZC010000350         2942 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1299972, whole genome shotgun sequence.
ACCESSION   DBNGZC010000350 DBNGZC010000000
VERSION     DBNGZC010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1299972"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="AC16G0_06320"
     CDS             <1..604
                     /locus_tag="AC16G0_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HIR5099274.1"
                     /translation="LTSRRAIPVLASNDSGSIASLQAGVIVDARLRKRERSSSRITDA
                     PLVIGLGPGFTAGIEAHLVIETNRGPNLGRIITQGEADPYTGEPIEIAGHGKDRYLYA
                     PHAGIFRTRLDLGDQIVAGQVMGNVEGESLVAKVGGIVRGIIKDGLFVQAGAKLVDID
                     PRGDPRSLSELSRKAWTIADAVVTAIRQSRMINDEFSMGR"
     gene            complement(617..1735)
                     /locus_tag="AC16G0_06325"
     CDS             complement(617..1735)
                     /locus_tag="AC16G0_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR5099275.1"
                     /translation="MKIETLAVHVGREVDRTTGAVTPPIHLSTTFEREVDGSYPRGYI
                     YSRTSNPNRDALEKCLAVLEGGTAAAAFSSGSAASMGVFQALQPGDHVIAPTDVYHGT
                     ARLLQELFAPWGLETTFVDMTDPAQVRRAVRKTTKLIWVETPSNPLLKITDISAISEI
                     THDIGAVCVCDNTWATPALQRPFELGADLVMHATTKYLCGHSDVLGGALVAKDENEFL
                     QRIRKIQIGGGAVPSPFDCWLVLRGISTLPYRMRAHSETALTVAAYLSLHPGVEAVHY
                     PGLTGHAGYEVAVRQMALPGGMLSFQVNGGREKAFDVAAKVRLFTRATSLGGAESLIE
                     HRASIEGPETRTPQNLLRVSIGLEHADDLVADLAQALS"
     gene            complement(1976..2920)
                     /locus_tag="AC16G0_06330"
     CDS             complement(1976..2920)
                     /locus_tag="AC16G0_06330"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5099276.1"
                     /translation="MEVRRVAVAGTGMMGPGIAATFALAGYEAIIVSRKAENAAKGVA
                     TARSLVSTLAENGLAEPEAAKAALSRFSPSTDPEAVACSAQLFVESIAENLAIKQEYF
                     ARLARAAPDTVLCSNTSGISITEIAAKSSRPERILTTHFWNPPYLMPLVEVVVGQRTD
                     LGIAQSVVGLLKGCGKVPVLVRKDVPGQLGNRIQHAMIRECMHIVQEGIATAEDVDLA
                     VKAGVGLRTPVYGVYEHADMVGLDLVKAVQDYIVPDLSTVKGSARIHQEKVLKGELGV
                     KTGRGFLDWPAGKGDQVKARRDEFLMQFLRWEKEGRFA"
BASE COUNT          528 a         1004 c          905 g          505 t
ORIGIN      
        1 cctcacgtca cgccgagcca ttccggtcct cgccagcaac gatagcggct ccatcgcctc
       61 cctccaagcg ggtgtgatcg tggacgcccg ccttcgcaag agggaacgga gcagttcccg
      121 gataaccgat gcccccctcg tcatcggact tggacccggt ttcaccgccg ggatcgaggc
      181 ccatctcgtc atcgagacaa atcgcggtcc gaacctcggc cggatcatca cccagggcga
      241 ggcggacccc tacaccggcg agccaatcga gatcgccggt cacggcaagg accgatatct
      301 gtacgcgccg cacgcgggga tcttccgcac ccggctggac ctcggtgatc agatcgtcgc
      361 gggacaggtg atgggcaacg tcgagggtga gtctctggtt gccaaggtcg gcggaatcgt
      421 tcgtgggatc atcaaggacg ggttgttcgt gcaggcgggt gccaagctgg tggatattga
      481 ccctcggggc gatccccgct ccctctcaga actctcccgg aaagcctgga ccatcgccga
      541 cgcggtcgtt acggccatcc gtcaatcaag aatgatcaat gacgaattct caatgggacg
      601 ctgatcccct cggctgttac gagagggcct gcgccaggtc cgccacgaga tcgtctgcgt
      661 gctcgaggcc gatggagaca cggaggaggt tctgaggcgt ccgggtctcc ggaccctcga
      721 tcgaggcgcg atgctcgatc aggctttcgg cgccgccgag actcgtcgcg cgtgtgaaga
      781 gcctgacctt cgcggccacg tcaaaagcct tctcgcgccc gccattcacc tgaaaggaca
      841 gcatcccgcc gggcagcgcc atctgccgga cggccacctc gtacccggca tgtcccgtga
      901 gacccggata atgcaccgcc tcgacacccg ggtggaggct caagtacgct gcgactgtta
      961 gcgcggtttc ggagtgagcg cgcatacggt acggcaaagt cgaaatcccc cgcagcacca
     1021 gccagcaatc aaagggggac ggaaccgcac cgccaccgat ctggattttg cggattcgct
     1081 ggagaaactc attctcgtcc ttggcgacca gggcgccccc caggacatcg ctatgcccgc
     1141 agaggtattt cgttgtggcg tgcatgacca ggtcggcgcc aagttcgaag gggcgctgca
     1201 gagccggagt ggcccaggtg ttgtcacaga cacaaaccgc tccgatatcg tgggtgattt
     1261 cggagatcgc cgagatatcc gtgatcttga gcaggggatt cgagggcgtc tcgacccaga
     1321 tcagcttggt agtcttccgc accgcccgtc tgacctgagc cgggtcggtc atgtccacga
     1381 aggtggtttc cagaccccac ggggcaaaga gctcctgcag gaggcgggcc gtcccgtggt
     1441 acacgtcggt tggcgcgatg acgtggtcgc ctggctggag cgcctggaac acccccatgc
     1501 ttgcggccga gccggaggaa aaggcggcgg cggccgttcc cccctccaac accgcgagac
     1561 atttctccag cgcgtcccga ttgggattgc tggtccgcga atagatgtac ccgcgggggt
     1621 agctgccatc gacctcgcgc tcgaaggtgg tggacaggtg gatcggaggt gtcacggctc
     1681 cggtggtccg atcaacctca cggccaacgt ggactgccag cgtctcgatt ttcattcgcg
     1741 atccctcatc accttccggc aacgcacacc gctgtcgtcg ctgaaggggc ggtgaccgcc
     1801 cgagcggtac gcttgacaac tccgatgacg ttcactccgg cccctttccc ggcctcttcg
     1861 tcaacctccc catctccctc tcgggggaga agcaaacgac gaggggcaaa aaacacgacg
     1921 ggcgccaccg caggcactct caccgcttgg gcgcccgtcg ctggaccagt gacgatcagg
     1981 caaagcgtcc ttccttctcc caccggagga actgcatcag gaactcgtcg cggcgggcct
     2041 tgacctggtc ccccttgccg gcaggccagt ccaggaaccc gcgtcccgtc ttcacgccca
     2101 gctcaccctt caacaccttc tcctgatgaa tgcgcgccga gcccttcacg gtgctaagat
     2161 cggggacgat gtaatcctgc acagccttca ccaggtccaa ccccaccatg tccgcgtgct
     2221 cgtacacacc gtacacaggg gtgcggaggc cgacacccgc cttgaccgcc aggtccacat
     2281 cctctgccgt ggcgatcccc tcctgcacga tgtgcatgca ttcgcggatc atggcgtgct
     2341 ggatccgatt gccgagctgg ccgggcacgt ccttgcgcac gagcacggga accttgccgc
     2401 agcccttgag caaccccacc acgctctggg cgatcccgag gtcggtccgc tgccccacga
     2461 ccacctcgac cagcggcatc aggtagggcg gattccagaa gtgcgtcgtg aggatccgct
     2521 ccggccggct ggacttggcc gcgatctcgg tgatgctgat ccccgaggtg ttggagcaca
     2581 gaacggtgtc cggggcggcg cgggccaacc gggcaaagta ctcctgcttg atggccaggt
     2641 tctcggcgat ggactccacg aacaactggg ccgagcacgc caccgcctcc ggatcggtag
     2701 acggactgaa gcgcgacagc gcggccttgg ccgcctccgg ctccgccagg ccgttctccg
     2761 ccagcgtcga gacgagcgac cgcgccgtcg ccacaccctt ggcggcgttc tccgccttcc
     2821 gactgacgat gatcgcctcg tagcctgcca aggcgaacgt ggctgcgatc ccggggccca
     2881 tcatgccggt cccggccacg gcaacgcgac gaacttccat gcgaggctcc cttcacggaa
     2941 ga
//
LOCUS       DBNGZC010000351         5190 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2572155, whole genome shotgun sequence.
ACCESSION   DBNGZC010000351 DBNGZC010000000
VERSION     DBNGZC010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2572155"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..530
                     /locus_tag="AC16G0_06335"
     CDS             129..530
                     /locus_tag="AC16G0_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR5099277.1"
                     /translation="MRVNRAVVLSLVGLAVLSFGCSRLFKDRLSDGNAPADLKRVTFE
                     IVARDNRCEPAILAADRDGRSLLVTFQVTSVGKPHVFQIPDLGIRMTIPADNQIAIPL
                     IADRSGIYAYACNSVPWISPLMTSTGKLAIK"
     gene            584..1822
                     /locus_tag="AC16G0_06340"
     CDS             584..1822
                     /locus_tag="AC16G0_06340"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:NF013755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HIR5099278.1"
                     /translation="MPEPSQAIRSPEQPPIWIRTPEALREMVARTAGARILAVDSESD
                     SLHHFPEKVCLVQVASEDGAVYLVDPLSLPDLSPLGPTLADPAIVKVFHGASYDLSSM
                     KRDFGFEFAGIFDTMLAGQFLGLPELGLNALLQGFLGITPGRSRQKDDWAKRPLQPGQ
                     EAYAAEDVRHLIPLRERLQAGLQARGREAWLEEECQALVALPPAERVFDPEDYLRVKG
                     AKDLSRRSLAVLRELFLARETWAHEAGRPPFKVLGNESLIRVATGLPRTLKALLQIPG
                     CSQKVVGRYGTGVLAAVARGLAIPEEDLPFYPRPKKPRLSPAVLRRIEALVAWRAEAA
                     GRLGLDAGLVLPRRLIERLAEEMPVTPDNLGRVEGLRRWRADTFGAEILHILASPPEE
                     RSREPRPAASSESGERKETA"
     gene            1819..3192
                     /gene="holA"
                     /locus_tag="AC16G0_06345"
     CDS             1819..3192
                     /gene="holA"
                     /locus_tag="AC16G0_06345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR5099279.1"
                     /translation="MTGGVEKAVLGIKQGKRYPVYLLHGDEFLTRGGAKAIIQALVPA
                     GQEAWSVETIVEEADLASLPLRLNTLPLFGGTKVVAVYDSKAFLSKQTAGNLTEKSLE
                     AWQAGDQPRAVRLLLQVVAAAGGDAAFLERAARGESSESEWKGILSWTPDPEREAWLR
                     ETAGRAVADGAAVPEKTGSGLAQIYEETLLRGISPAAALVLTAEVVDQRRTLFKRLSD
                     LGFVIDCGVRSKRAWETQMNPEMARAKIREVVKDAKKSIDPDAVTAIVDRTGASVRAL
                     QSELEKILLYIGTRPTVTTTDVMAVLSSSREANVFDLTNALGSRDAGQALDALRSLFA
                     QREPIPRILGTIANEIRSLLVARCVLDARFAGQIDRAMAFPAFQARMLPRLKEASEGD
                     DGSAAQLAEMHPFRAFQMLRSASRFSQAELLRALEMIHETDLALKTSGHPESLLLEQL
                     LITMCGG"
     gene            complement(3330..3809)
                     /locus_tag="AC16G0_06350"
     CDS             complement(3330..3809)
                     /locus_tag="AC16G0_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099280.1"
                     /translation="MIDLHTDERGRLVCAMCGATECYIHRITGLWEKAPDEAWDGYIK
                     GIIRVPSKTSAYAPFVFLTAGSPDEEVTGIRFSYFRSSGPNGRIADGPGPGGAPVLDQ
                     LDLFHLLDRLSAFGFIRPKDVEVLGARFRRDFPGYSSGQTRLSEPDGGEQGATQYLQ"
     gene            complement(3963..4091)
                     /locus_tag="AC16G0_06355"
     CDS             complement(3963..4091)
                     /locus_tag="AC16G0_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099281.1"
                     /translation="MGDSIILYGCWVLQGLVIFLWARWAELERKHSDEGDWVFYLE"
     gene            complement(4627..4806)
                     /locus_tag="AC16G0_06360"
     CDS             complement(4627..4806)
                     /locus_tag="AC16G0_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099282.1"
                     /translation="MSHILVDDTDGTSVLHEVEYTISPHRDGLVRYTKVPFSERPCTC
                     PEREVHKRPSAEAAK"
BASE COUNT          941 a         1601 c         1708 g          940 t
ORIGIN      
        1 tgcggtgggc ggaaaatatc agagggcgga gagccgcgac aatcgggaat tggcgaacca
       61 agtgaaagtg gatcattgca acttggcaga ccgcgtgcta ggctctgccg gcttggaggg
      121 ttccagaggt gcgcgtgaat cgggccgtgg tgttgagcct ggtgggactg gcggtgcttt
      181 ccttcggatg cagccgtctg ttcaaagacc ggctctcgga cgggaacgcg ccggcggacc
      241 tgaagcgcgt caccttcgag atcgtggctc gggacaaccg gtgcgagccg gccatcctgg
      301 cggctgaccg cgatggccgg tccctcctgg tcaccttcca ggtcacgagc gtggggaagc
      361 cgcacgtgtt ccagattccg gatttgggta ttcgcatgac gatcccggcc gacaaccaga
      421 ttgccatccc gctgatcgcg gatcgatcgg ggatctacgc ctatgcctgt aattccgtgc
      481 cctggatcag cccgctgatg acctcgacgg gaaagctcgc catcaagtag catcctgtcg
      541 ctccagacgc ctgttcgagt gttgcggacc cgcgccatcg accatgcccg aacccagtca
      601 agccattcga tctcccgagc aaccgcccat ctggatccgg acccccgagg ctctccgcga
      661 gatggtggcc cggacagcgg gagcgcgaat cctcgccgtg gattcggagt cggacagcct
      721 gcaccacttt cccgagaagg tctgcctcgt gcaggtggcc tccgaggacg gcgccgtcta
      781 cctggtggac cccctcagtc tgccggacct cagccccctc ggaccgaccc tggcagaccc
      841 cgccatcgtg aaggtgtttc acggggcctc ctacgacctc tcctccatga agcgggattt
      901 cggcttcgag ttcgccggga tcttcgacac gatgctcgcg gggcagttcc tgggccttcc
      961 ggagctgggc ctgaacgccc tgctccaggg gtttctgggg atcacgccgg ggagatcgcg
     1021 ccagaaggac gactgggcca agcggcccct tcagcccggg caggaagcat acgccgcgga
     1081 ggacgtccgg cacctcatcc ctctccgcga gcggttgcag gccgggctcc aggcgcgggg
     1141 gcgggaggcc tggctggaag aagagtgcca ggccctggtg gccctccctc ccgccgagcg
     1201 cgttttcgat ccggaggatt acctgcgggt gaagggggcg aaggacttga gccgccggag
     1261 cctcgccgtc ctgcgcgagc ttttcctggc ccgcgaaacg tgggcgcacg aggcgggccg
     1321 gcctcccttc aaggtcctgg ggaacgagtc gctcatccgc gtggcgacgg gacttccgag
     1381 gaccctcaag gccttgctgc agattccggg ctgttcgcag aaggtcgtgg ggcgctacgg
     1441 taccggcgtg cttgccgccg tggctcgagg cctggcgatc ccggaggaag acctcccctt
     1501 ctatccccgg ccgaagaagc cccgcctgtc tcctgcggtc ctgcggcgga tcgaggccct
     1561 ggtcgcgtgg cgggcggagg ccgccggccg cctcgggttg gatgccggcc tggttttgcc
     1621 gcggcgactc atcgagcggt tggccgagga gatgcccgtc acgccggaca atctgggacg
     1681 agtcgagggg ctccgccgct ggcgtgcgga tacgttcggg gccgagattc tgcacatcct
     1741 ggcgtcgccc cccgaggagc ggagccgcga gccgcgcccg gctgcgtcga gtgaaagcgg
     1801 ggagaggaag gaaacggcat gacgggaggc gtggagaagg ctgtcttggg gatcaaacaa
     1861 ggaaagcgct acccggtcta tcttctccat ggcgacgagt ttctcacacg cggcggggcc
     1921 aaagccatca tccaggcgct ggtgcccgcc ggccaggagg catggagcgt ggagacgatc
     1981 gtggaggagg ccgacctggc cagcttgccg ctccggctga acaccctccc gctcttcggt
     2041 ggtaccaagg tggtggccgt gtacgatagc aaggctttcc tctccaagca gaccgccggc
     2101 aacctcacag agaagagcct ggaggcctgg caggcggggg atcagccccg ggccgtgcgg
     2161 cttctcttgc aggtggtggc ggcagcggga ggggacgcgg ctttccttga gcgtgcggcc
     2221 cgaggagaga gctctgaatc cgagtggaag ggaatcttgt cctggacgcc ggatcccgag
     2281 cgagaggcct ggcttcgaga gaccgccggc cgggcggtgg cggacggggc cgcggtcccg
     2341 gaaaaaaccg ggtccggatt ggcgcagatc tacgaggaga cccttctgcg cggtatctcg
     2401 cctgccgccg ctttggtcct tacggccgag gttgtggacc agcgccggac gctcttcaag
     2461 cggctcagcg accttggctt cgtcatcgac tgcggtgtcc ggtccaagag ggcctgggag
     2521 actcagatga acccggagat ggcacgggcc aagatccggg aagtggtaaa ggacgccaag
     2581 aagagtatcg atcccgatgc ggtgaccgcc atcgtggatc gcacgggagc cagcgtgcgc
     2641 gccctccagt cggaactgga gaagatcctg ctgtatatcg gtacccgccc cacggtcacc
     2701 acgacggacg tcatggccgt gctgagcagc tcccgggagg cgaacgtctt cgaccttacg
     2761 aatgccttgg gtagccggga tgccggccag gcgctggacg ccctgcgcag ccttttcgcg
     2821 cagcgagagc cgatcccgcg gattctcggc acgatcgcca acgagattcg gtcactgctc
     2881 gttgcccggt gcgtcctgga tgcccggttt gccgggcaaa tcgaccgcgc catggccttc
     2941 cccgcctttc aggctcggat gcttccccgc ttgaaggagg cgagcgaggg ggacgacgga
     3001 tcggcggccc agctcgcgga gatgcacccg ttccgggctt tccagatgct caggagcgct
     3061 tcccggttct cacaggcgga gctgcttcgg gccctggaga tgatacacga gacggacctg
     3121 gccctgaaga cttccggcca tcccgaaagc ctcctcttgg agcagctcct gatcacgatg
     3181 tgcggcgggt gatccgggcc ctccggcctg acaggttctt gccatgtcag cggacggact
     3241 cgccgcctga atgtcctcgg ctcgcgtgga attgcctcgt ccggacggag cacggtggcc
     3301 aggagtcacg agtctgggaa gtacgccggt cattgcaggt attgtgttgc cccttgctcc
     3361 ccaccgtctg gttcgctcag tctggtttgc cccgatgaat agcccgggaa atcccggcga
     3421 aagcgagccc cgagcacctc gacatctttg ggcctgatga agccgaaggc actgagtctg
     3481 tcgagaagat ggaacaggtc gagctgatct aacacgggtg caccgccagg gccaggacca
     3541 tccgcgatcc gcccgttggg tccggaggat ctgaaatagc tgaagcgaat tcctgtgacc
     3601 tcttcatctg gcgaaccggc ggtcaggaac acgaagggcg catacgccga ggtcttcgag
     3661 ggaacgcgga taatcccctt gatatacccg tcccaagcct catcgggtgc cttctcccag
     3721 agaccggtga tgcgatggat gtaacactct gtggcgccgc acatcgcgca gaccagccta
     3781 ccacgctcat ccgtgtggag gtcgatcaaa tcagtgatgg accccaccca ggtatccgtc
     3841 cggcataacc ggcactcgat ttgatacgcc atggaccgtc tccattccgg gagtccaaga
     3901 aataccgggt cctgagtcag atttcctgaa gtaaaattgt ctcctggatc cgccaggttg
     3961 agctattcca agtagaacac ccaatcgcct tcgtcggagt gcttcctctc cagttcggcc
     4021 cagcgtgccc acagaaagat caccagaccc tggagaaccc agcagccata taggatgatc
     4081 gaatccccca tgggcgtcct ccttccggct ccgcacctgc cgggcagtaa ggcaactgcc
     4141 gtgccgtcgc gagcatggta cagggtccgg caggcaaggg aatgcgtatc tccgcgtcgt
     4201 tatgcggagg ggcgtgcagg ggcccgccgg gatcgtcaaa gggccatctc ctcccgtctg
     4261 cgaaattgcg tatctgaaaa gattcgccga ccgaatcggt tggatacagg ttggaaagcc
     4321 aatgtgaagg cgcagttgaa agatagaagg caggtcagag gtaatagctg aaccatgaat
     4381 tcctgccgga gccccatggg cagctttccc tgggttcgcc aaggtctttc ctggtcattc
     4441 cgcgggagcc tcccaaaagg ccatcagcgg tctgtccgag ttggagcagg tgctggcgca
     4501 gatcgtggtt gaggaggaat ttacggtgga gcggacgtca ccagggcagg caggggcgtt
     4561 caagagcgac agtcttcaca ctccggcggc gctcggaccg cagcctctct acatgatgct
     4621 ggcgtgctac ttcgcggcct cagccgacgg tcgtttgtgg acttcccgct ccgggcaggt
     4681 gcaggggcgc tcggagaagg ggaccttcgt gtagcgcacc aggccatcgc ggtgagggga
     4741 gatggtgtac tccacctcat gcagaacgga cgtaccgtcc gtgtcgtcga ccaagatgtg
     4801 actcatgatc ctgtccacgg tgtctcccct ccctgtccgc ctcaatgatt ggcgtccgcc
     4861 gcatgaggag cgggatcact tacaaagaag ataggcgacc aggaacggag atggcatacg
     4921 ttaggataca caattcacaa agaaagttgc ctgattctgc cggattatca ggcgcaagag
     4981 agctatcacg taaccccaga gggtgctgaa aagtccttct tcggcagcgt gttggaagga
     5041 ggaggcaagg gcgtattgga aggaggcggg agcttccatg tgccgtcgct catcattttc
     5101 tgccgggcaa atatctttcc tgccccgccc ctgccatctt tttcaacccg atgacgcaga
     5161 cactctcagc ggctccgagc atggtctcat
//
LOCUS       DBNGZC010000352         3164 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2279152, whole genome shotgun sequence.
ACCESSION   DBNGZC010000352 DBNGZC010000000
VERSION     DBNGZC010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2279152"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2431
                     /locus_tag="AC16G0_06365"
     CDS             <1..2431
                     /locus_tag="AC16G0_06365"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HIR5099283.1"
                     /translation="ATHGVIYVRTEYPLAIKHALIALRQARELGLLGASILGTSFDFD
                     IEIVRGAGAFVCGEETALIRSIEGRMGEPRQRPPFPVARGLGGNPTCINNVETLANIP
                     LIITQGAEAFRRIGVPENSGTKIFSLVGKIRNTGLVEVPLGTRIRDVVYEIGGGAPGK
                     GRLKAVQTGGPSGGCIPARLFDLSIDYDSLAEAGAIMGSGGMIVMDESTCMVDMAKYF
                     MSFLKAESCGKCFTCRKGTQRMYEILEDVTTGKATLAHLDLLEELAQVVRDTTLCGLG
                     QSAPNPVLSTLRYFRHEYERHITHKRCDAYVCKDLVGAPCQAACPLGTEAWRYVAHIA
                     RGEYDEAYRVIREANPFPSVCARVCHHPCEERCRAGTGGSEPVAIRALKRWVAERVDP
                     ASYQPRASRSPGTNPDPVAVIGAGPAGLTAAHELALKGYRVTIFESEAEAGGVLLRGI
                     PSYRLPKEVLRKEIRSLLSYGNLTLEYRRALGRDFCLDDLFARGFRAIFLALGAPKSW
                     RLNLPGEESQGVYPAMEFLSWSNRQGEQFVRGHVGVIGGGNAAVDAARLALRQPDVSA
                     VTILYRRARADMPAFAEEVEAALEEGVRLEPLVSPVRILSDSGRVTGVVCVRNRLAGM
                     DGTGRRSPVALPGSEHTIPLDTLIIAVGEAVDGEVLRSAGLAAGEDGGIAVDPDTLAT
                     SRQGVFAGGDVVTGPNTVVEAIAAGKKAAVMMDRFLRGEELRQPARIVLPGTFIPPAS
                     PLEGEPLTARRVQPPRLPLAARQRSQQEIEKPFSLEEAAREARRCLRCDLEFTGAREE
                     AVPAAMGGATP"
     gene            2428..3144
                     /locus_tag="AC16G0_06370"
     CDS             2428..3144
                     /locus_tag="AC16G0_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR5099284.1"
                     /translation="MISLSLNGLPVSVEEGATLLEAARFFGFPIPTLCHLDGLTPYGA
                     CRLCVVEIGEGPRSKLVSACTYPAENGLKVRTSSGRVIRARKMILELLLASCPQSKTI
                     QDLASAYQVRQQRFRQEHEDCILCGRCVRMCAEQMVAGAIDFRGRGEKRNIGTPFDMR
                     SEVCRLCGGCMYVCPACQLRCTYTEPDKAICGGCANLSPPCLAKEPFDDMMCYMAPCV
                     ACEIPKETAPQEGGRCHSQG"
BASE COUNT          522 a         1051 c         1077 g          514 t
ORIGIN      
        1 cgcgacgcac ggcgtcatct acgtccgcac cgagtacccc ctggccatca agcatgccct
       61 gatcgccctt cggcaggcga gggagctggg gctcctcggg gcgagcattc tcgggacctc
      121 gttcgatttc gacatcgaga tcgtgcgggg ggccggggcc tttgtctgcg gggaggagac
      181 ggcactcatt cgctccattg aggggcgtat gggcgagccg cggcaacggc ctccctttcc
      241 cgtcgcgcga ggtctcggcg gaaaccccac gtgcatcaac aacgtggaga ccctggccaa
      301 cattcccctg atcatcaccc agggtgcaga ggccttccgc aggatcggcg tgcccgagaa
      361 ctcgggaacc aagatcttct ccctcgtggg caagatccgg aacaccggac tggtggaggt
      421 gccgctcgga acccgcatcc gagacgtggt ctacgagatc ggcggaggcg cccccgggaa
      481 gggccggcta aaggccgtcc aaacgggcgg cccctctggc ggctgcatcc cggcgcgcct
      541 tttcgatctg tccatcgact acgacagtct ggccgaggca ggggcgatca tgggctcggg
      601 cggcatgatc gtcatggacg agagcacctg catggtggac atggccaagt acttcatgag
      661 cttcctcaag gcggagtcct gcggcaagtg cttcacctgt cggaaaggca cacagcggat
      721 gtacgagatc ctggaggatg tcacgacggg caaggctacc ctcgcccact tggatctcct
      781 cgaagagctg gcccaggtag tgagggacac cacgctctgc ggcctcggcc agtccgcccc
      841 caaccccgtg ctgagcaccc tgagatactt ccgccacgag tacgagcgtc acatcacgca
      901 caagcgctgc gacgcctacg tctgcaagga cctggtcggg gcgccctgtc aggcggcgtg
      961 ccccctgggg acggaggcgt ggcgctacgt agcccacatc gcacgggggg aatacgacga
     1021 ggcgtaccgc gtgatccgag aggctaaccc cttcccctcg gtctgtgccc gcgtgtgcca
     1081 ccacccctgc gaggagcgat gtcgggccgg gaccggtggc tccgagccag ttgccatccg
     1141 ggccttgaag cgctgggtgg ccgaacgcgt ggatcccgcc agctatcagc cccgcgcctc
     1201 acgcagcccg ggcacgaacc ctgaccctgt ggccgtaatc ggtgcgggcc cggccggcct
     1261 cacggccgcc cacgagctcg ccctcaaggg ctatcgcgtc accatcttcg aatccgaggc
     1321 cgaggccggc ggcgtgctcc tccgcggcat tccttcctat cggcttccca aggaggtgct
     1381 gcgcaaagag atccggtcgc tcctgagcta cgggaatctg acgctggagt accggcgagc
     1441 cctgggacgg gacttctgcc tggatgatct cttcgcccgc ggattccggg ccatcttcct
     1501 ggcgctggga gcgccgaaga gctggcggct gaatctgccg ggggaggaga gccagggggt
     1561 ctaccccgcc atggagttcc tgagctggtc gaaccgacag ggcgagcagt tcgttcgggg
     1621 ccatgtgggc gtcatcggag gcggcaatgc ggccgtggat gcggcgcggc tcgccctccg
     1681 gcaacctgac gtcagcgccg tgaccattct ctaccggcgc gcccgcgcgg acatgccggc
     1741 ctttgccgaa gaggtcgagg cagccctgga agagggggtg cggctcgaac ccctggtgtc
     1801 gccggtgcgg attctctccg actccggccg ggtgacggga gtcgtgtgcg tcaggaaccg
     1861 tctggccggc atggacggaa ctggccggag gtcaccggtc gcgcttcccg gcagcgagca
     1921 caccattccc ctggatacct tgatcatcgc cgtcggcgaa gcggtagatg gggaggtcct
     1981 ccggtcggcg ggcctcgcgg cgggagagga cggaggaatt gcggtagacc cggataccct
     2041 ggccacgagc cgccaggggg tgttcgcggg aggagatgtg gtcacaggac caaacacggt
     2101 ggtcgaggcc atcgcggccg gcaagaaggc cgcggtcatg atggatagat tcctcagggg
     2161 ggaggagctg cgccagccag cccgtatcgt cctccccggc accttcatcc cgcccgcctc
     2221 gcccctcgag ggtgagcccc tgactgctcg ccgggtgcag ccccccaggc tacccctggc
     2281 cgctcgccaa aggtcccagc aagagatcga gaagcccttc agcctggagg aggccgcgcg
     2341 ggaggcccgg aggtgcctgc gctgcgacct cgagttcacc ggggctcgcg aggaggcggt
     2401 cccggccgcc atgggaggcg cgacgccatg atcagcctga gcctcaacgg gctgcccgtc
     2461 tcggtggagg agggcgccac gctgctcgag gccgcccgct tcttcggctt tccgatcccc
     2521 accctctgcc acctggacgg gctcacgccc tacggtgcct gccgcctgtg tgtggtggag
     2581 atcggcgagg gcccacgctc gaagctggtc tcggcgtgca cctacccggc cgaaaacggg
     2641 ctcaaggtgc ggacatcctc gggccgtgtc atccgggcac gcaagatgat cctggagctt
     2701 ctcctggcct cgtgccccca gtcgaagacg atccaggacc tcgcatccgc gtatcaggtc
     2761 cgtcagcagc gctttcgcca ggagcatgaa gactgcatcc tgtgtgggcg gtgcgtgcgc
     2821 atgtgcgcgg agcagatggt ggccggagcc atcgactttc gcgggcgagg cgagaagcgg
     2881 aacatcggca caccgttcga catgcgctcc gaggtgtgtc ggctctgcgg cggttgcatg
     2941 tacgtgtgcc cggcatgcca gctccgctgc acctataccg agccggataa ggcgatctgc
     3001 ggcggctgcg cgaatctgag tccgccctgc ctcgccaaag aaccgttcga cgacatgatg
     3061 tgctacatgg cgccttgtgt cgcctgtgaa atcccgaaag agacagctcc acaggagggt
     3121 ggcagatgtc attctcaagg ataaatgctt cggccgccac gctg
//
LOCUS       DBNGZC010000353         2430 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_324197, whole genome shotgun sequence.
ACCESSION   DBNGZC010000353 DBNGZC010000000
VERSION     DBNGZC010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_324197"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..2430
                     /locus_tag="AC16G0_06375"
                     /pseudo
     CDS             172..2430
                     /locus_tag="AC16G0_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743209.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MGH1-like glycoside hydrolase domain-containing
                     protein"
BASE COUNT          445 a          778 c          790 g          417 t
ORIGIN      
        1 cacacactct tcgtggacgg cggcatgacg ctgtatcccg gatttgccca tgggggatag
       61 agcagctcgg gcggcgggga ggggcggcag gcggatgagg gggggagcgg cgcagagcgt
      121 cagtcgccat acgtgggacg tgaaatggaa ggcgaaaggc ggaaagcgga aatgatcgcg
      181 gagctcaaga gacttgagga ggcccgcgag cggaccgcgc actggagacg gtgggggccg
      241 tatctctccg aacgacagtg ggggacggtc cgggaggatt actcggcgca cggcacagcc
      301 tgggaatact tccctcacga ccaggccaga tctcgagcct accgctgggg ggaggacggc
      361 atcctgggga tttgcgacaa tcaccagcgt atctgcttcg ctcccgcctt ctggaacgag
      421 taagatccca tcctcaaggc acgcctgttc ggtctcagcg gctcccaggg aaaccatagc
      481 gaggacgtca aggaatacta cttccacctc gactccacac ccacccattc ctacatgcag
      541 ggtctctaca agtaccccca aggagagttc ccgtacgccc gcctcctgga ggagaaccgc
      601 aagcgctccc gccgggagcc ggaattcgaa ttgttggaca cgggtgtgtt cgatggggac
      661 cggtacttcg atgtcttcgt ggaatatgcc aaggcggcca tcgacgacgt cttgatccga
      721 atcacggtat ggaaccgggg accggaaccg gccagcctcc atcttctccc gacgctctgg
      781 ttccggaaca tctggagctg ggagcatggg accgcccggc ccaagctctg gcaggctcca
      841 tcggccgaag ggacggccgc cgtggtggcc gagcacccgg ggtttggatc cccgtaccgg
      901 ctcatcgccg aaggggcgcc cgatctcctg ttcaccgaga acgagagcaa ttttcagcgt
      961 gtgtggggca ccgcaaaccc gcagcccttc gtcaaggacg ccttccatga atacctggtt
     1021 catgggcgga aggacgcggt gaaccccgcg cggggtggga cgaaggcggc ggctcattac
     1081 cctctccggc tggcgccggg ccagagcgcg gtgatccgtc tgcgcctcac cgatggtgaa
     1141 cccggcaggg cccccttggg ggggccgttc gagcagttct tcgcggcgcg ccagcgtgag
     1201 gcggacgagt tctatgccag cgtgatcccg aggcaactga gcgacgacgc gaagggcgtc
     1261 atgcgccagg cgctggcggg catgctctgg tccaggcagt ggtatcacta cgatgtccgg
     1321 cgctggctcg agggcgaccc cacgcagccc ccgcccccgc cgggacggga gcagggccgg
     1381 aaccatgagt ggacgcacct ctacaacgag gacgtcatct cgatccccga caagtgggaa
     1441 tacccctggt acgccgcctg ggatctggcc tttcacaccc tgtccctggt tctggtggat
     1501 ccggacttcg ccaaagacca actgatcctc ctcttgcgcg aatggtacat gcacccgaat
     1561 ggccagatcc ctgcctatga gtggggattc ggggacgtga acccctgcgt ccatgcctgg
     1621 gcggcctggc gggtctacaa gatcgaaggg cggatccgcg gccagggcga ccgccagttc
     1681 ttggagcggg tcttccacaa gctgctcctg aacttcacct ggtggatcaa ccgcaaggac
     1741 ccccatgggc tcaacgtgtt ccaggggggc ttcctgggtc tggacaacat cggggtcttt
     1801 gaccgggggg caccgcagcc gaacggcggg cctctcgagc agtccgacgg caccgcctgg
     1861 gtggggatgt tctgcctcaa catgttggcc atcgctctgg agctcgcccg ggagaacccc
     1921 gcctacgagg acgtggcgtc gaagttcttc gagcatttcg tgtacatcgc tcacgccatc
     1981 aacaacatcc accgcgaggg gctggcgctc tggaacgagg aggacggatt cttttatgac
     2041 gtcctccacc tgccggacag ctcggccctg cccctcaagg tgcggtccat ggtcgggctg
     2101 atcccgctct tcgccgtcat gacgctggag cccgaggtgc aggactccct ccccaggttc
     2161 tggcgtcgga tgaaatggtt catcctgcac cggccgtggc tcgtcgagca cgtggtgccg
     2221 cagaagactc cggagggcaa gacccggcgg ctgctctccc tggtgaacgg tgagcgcctc
     2281 cagcgggtcc tctgctacat gctaaaccag gacgaattcc tctcgcccca cggaatccgg
     2341 tccctctcgc gccaccatct cgaccatccg tacttcctca ggttgaacgg agacgagcac
     2401 tgcgtggact atgagccggc cgactccacc
//
LOCUS       DBNGZC010000354         2481 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2180705, whole genome shotgun sequence.
ACCESSION   DBNGZC010000354 DBNGZC010000000
VERSION     DBNGZC010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2180705"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="AC16G0_06380"
     CDS             <1..802
                     /locus_tag="AC16G0_06380"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR5099285.1"
                     /translation="RIRVMKGYDRLPPPSPAELRVLRRVGAPERVRLACQLRPTHDVS
                     VIPILPPQARPGDDLPGSDLLTQKEQEVTVLFADLRGFTRIAERKLPYDVVFLLNKYF
                     EAAGGSIKRAGGIANQFTGDGAMALFGVGVSPDEGSRHALAAAGDIVRTVAELSQTLA
                     EELSAPLKIGIGIHTGPAVVGRMGYAETVYLTAVGDTVHVASRLQELTKEYDCQLVIS
                     EQVARRANLDVSAFPRHELTVRNRVEPLAIRVIDEVSRILPGVPAAEA"
     gene            929..1738
                     /locus_tag="AC16G0_06385"
     CDS             929..1738
                     /locus_tag="AC16G0_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019989067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099286.1"
                     /translation="MPKRRRSLKEMAGDPRFIPGIYNYCDRWCERCPLTSRCLVYATE
                     QEQDAEDPASRDINNRAFWERMEGIFREAHEMLDEMLKERGIELDPAELDAERVRERQ
                     RQDEQNHPCAIAARQYAKAVEDWVEDASPRLRERGETLTSHLRMGLPGADPEGDARRL
                     VDATDIIRWYQHQIAVKIMRAAHSARGEDEIDIQLDHSDADGSAKVALTGMDRSLAAW
                     SELLRQLPDDEDRILPLLAALGRLRKDVEAAFPGARAFIRPGFDTGDGPSA"
     gene            complement(1778..2230)
                     /locus_tag="AC16G0_06390"
     CDS             complement(1778..2230)
                     /locus_tag="AC16G0_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011229257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR5099287.1"
                     /translation="MATYYEDFQVGQEWTSPTRTITEADVTAFAMLSGDWDEIHTSED
                     FARKESAYKTRIAHGMLGLALVEGLKKRIPAFADTRYIASLEWTWKFTGPILIGDTVH
                     VTIRINNKRESKSRAERGIIWEGGRMINQRGEVVQEGEHSLMIWRRPR"
BASE COUNT          410 a          799 c          863 g          409 t
ORIGIN      
        1 ccggatccgg gtcatgaagg gctacgaccg cctcccgccg ccgagccccg ccgagcttcg
       61 agtcctcagg cgcgtcggcg ctcccgaaag ggtccggttg gcctgccagc tccggccgac
      121 gcacgacgtc agcgtcatcc cgatcctccc acctcaggcg cgacccggcg acgacctgcc
      181 cggctccgac ttgctgaccc agaaggagca ggaggtcacc gtgctgtttg ccgacctgcg
      241 cggcttcacg cggatcgccg agcggaagct accctatgac gtggtgttcc tgctgaacaa
      301 gtacttcgag gcggcgggag gctcgatcaa gcgcgccggg ggtatcgcca accagttcac
      361 gggggacggg gccatggccc tgttcggcgt gggggtgagc cccgacgagg gcagtcgcca
      421 cgccctggcc gcggcgggcg acatcgtccg gacggtcgcc gagctcagcc aaaccctggc
      481 cgaggagctt tccgcgccac tcaagatcgg catcggtatc cacacgggac ccgctgtcgt
      541 gggccgcatg ggctatgccg agacggtcta cctgacggcg gtgggtgaca cggtgcacgt
      601 tgccagccgg ctgcaggagt tgaccaagga gtacgactgc caactcgtga tctcggagca
      661 ggtcgccagg cgcgcgaacc tcgacgtgtc cgcgtttccc cgacacgagc tgacggtccg
      721 caaccgggtc gagccgctgg cgattcgggt gatcgacgag gtctcccgca tcctgcccgg
      781 ggtgcccgcg gccgaagcct gacgccttgc gagcgggacg caggcgccgc ccggcgggtt
      841 ctggttaagg agggttgagg tcgattcgcc ttcgtgtccg tagcctggag gacacctgcg
      901 gaccagccgt tcagaagagg gaggcggcat gcccaagaga cgacgcagcc tgaaagagat
      961 ggcgggcgac ccgcggttca ttccgggcat atacaactac tgtgaccgct ggtgcgagcg
     1021 ctgcccgctg acgtcgcgct gcctggtgta tgccacagag caggagcagg acgccgaaga
     1081 tccggcctcg cgggacatca acaatcgagc cttctgggag cgcatggagg ggatattccg
     1141 ggaggcccat gagatgcttg acgagatgct gaaggagcgc gggatcgagc ttgatccggc
     1201 agagctggac gcggagcggg tgcgagagcg gcaacgccag gacgagcaga accatccgtg
     1261 cgccattgcg gccagacagt atgcgaaggc cgtcgaggac tgggtcgagg atgcgtctcc
     1321 gcgtctccgg gagcgggggg agacgctcac gagccatctc cgcatgggct tgcccggcgc
     1381 agaccccgag ggcgacgcgc gccgtctcgt ggatgcgacc gacatcatcc gctggtacca
     1441 gcatcagatc gcggtcaaga tcatgcgggc cgcccactcg gcccgaggcg aagacgagat
     1501 cgacatccag ctcgaccata gcgatgccga tggctcggcc aaggtggccc tgaccggcat
     1561 ggatcgctcc ctggcggcct ggtccgagct gttgcgccag cttcccgacg acgaggatcg
     1621 gatcctgccg ctcttggccg ctctgggccg cctgcggaaa gacgtcgaag cggcgttccc
     1681 tggcgcccgc gccttcatcc gtcccggttt cgacacgggg gatgggccct cggcgtgagc
     1741 gccgaggcat ccgtgacaaa atcccagatc gcagccgcta gcgggggcgg cgccagatca
     1801 tgagggagtg ctctccctcc tggacgacct caccccgctg gttgatcatg cggccgccct
     1861 cccagatgat cccccgctct gccctggact tgctctcccg cttgttgttg atgcggatcg
     1921 tcacgtggac cgtatccccg atgaggatgg gcccggtgaa tttccaggtc cattccaggg
     1981 aggcgatgta gcgcgtatcc gcgaaggccg ggatgcgctt cttcagcccc tccacgaggg
     2041 ccagacccag catgccgtgg gcgatgcggg tcttgtaggc cgactccttc ctggcgaagt
     2101 cctcgctggt gtggatctcg tcccagtcgc cggacaacat ggcgaatgcg gtgacgtctg
     2161 cctcggtgat cgttcgcgtg ggcgaggtcc attcctgacc gacctggaag tcttcgtaat
     2221 acgtggccat cgagtctcct ccatgcggca atgggtgtcg ggtgacggac gcgtcgggaa
     2281 tgtacccccg ccgtcaaggg gtgtcaattg gcgcgaggaa tggtccaggg ggagcggctc
     2341 ggccaggcgg cgtggtcaaa tcgcttgacg ggggcggttc tcgccgcaga gaatacgccc
     2401 gcaccgggtt cctgcattgg gctgccgtcc gtccggaaag gggagtggcc gacatgagcc
     2461 tgcctgagtt cagcctggat g
//
LOCUS       DBNGZC010000355         3559 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_420093, whole genome shotgun sequence.
ACCESSION   DBNGZC010000355 DBNGZC010000000
VERSION     DBNGZC010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_420093"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="AC16G0_06395"
     CDS             <1..734
                     /locus_tag="AC16G0_06395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5099288.1"
                     /translation="AATYAAALADRDQLKVKVHQVAAARAAVAQREAQLRLAEVQRSR
                     MTIRAPIAGLVTQRLANVGEVIGLGGGSMVTLTGPLFTLVDLDRMYVRATVDEFDARQ
                     IRLGQEARVTLEAAPGRTLRGRLYKISPAVSGERQEARTFNIRVALEEGKEFVKPGMS
                     ADVEVIVARRRDALFAPSQAIMEREGKKRVYVIADGRARATTVKVGESNWSSTEILEG
                     VREGDRVIINPDTPGLTEGARVRVE"
     gene            855..1592
                     /locus_tag="AC16G0_06400"
     CDS             855..1592
                     /locus_tag="AC16G0_06400"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099289.1"
                     /translation="MIAVEEVWRTYRMGPERVDALAGVTFHVAPGEFVAVCGPSGSGK
                     STLMNILGCLDRATRGRYTLEGVEVQSLDDDALAALRNRRIGFVFQTFNLLPRMTALR
                     NVELPLIYSGLPRPERQQSALEAMATVGLAARIGHRPSELSGGEQQRGAIARALVNRP
                     AIILADEPTGNLDSKAGDEILEVFHRLAEGGATTIMVTHNPRIAEQAARVILLSDGRI
                     VSDTRRAGTQRAEPLPDSNSVAAPLTS"
     gene            1623..2831
                     /locus_tag="AC16G0_06405"
     CDS             1623..2831
                     /locus_tag="AC16G0_06405"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099290.1"
                     /translation="MDLREAFSLAVEMLLANRVRSALTTLGVVIGVAAIILLVSLGEG
                     ATAYITREMTGLGTNLLSVTQGKTQTSGGFHPPMASTVRKLTFEDAMVLRRRATLLTD
                     IVPVVLGTGKVRHLGASRDISVIGVTPEFQRVRNLYVEIGQFVSEEDVEGRRRVVVLG
                     RTVKRELFGEENPLGQFVTLADSRYRVIGIMERKGVSLGFDIDDLVFIPTKSAQDLFD
                     TDHLFEILASVRSPEEVDQAIRQMKDILMRRHGNREDFTIVSQGAILSSFTTILKILT
                     AVLGGIAGISLLVGGIGIMNIMLVSVRERTREIGIRKAVGARRRDILGQFLLESVVLS
                     SLGGGLGILVGIGGAKGLSFFFTYLPTHVSLWAVLMAVGFSAAVGVFFGVYPARRAAS
                     LDPIQALRYE"
     gene            2955..>3559
                     /locus_tag="AC16G0_06410"
     CDS             2955..>3559
                     /locus_tag="AC16G0_06410"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR5099291.1"
                     /translation="MTERRAHRPAVMGRRGMVASAHPLASAAGLEMLWRGGNAVDAAV
                     AVAATLNVVEPFMSGIGGDGVLLLTLSRSAERKVLNFMGRTPFAARTEGCTPDELDRG
                     PKASLVPGNFAGWWEALRAHGRLPPSTVFAPAIRYADEGFPLTHRGVAFLAEHWRRLT
                     PQGGAVFCPGGEVPRPGTFLPHQALAATLRRLVAEGPDPLYR"
BASE COUNT          564 a         1104 c         1299 g          592 t
ORIGIN      
        1 ccgcggccac gtacgccgcc gcgctggcgg accgagacca gttgaaggtc aaggtgcacc
       61 aggtggccgc ggcccgggct gccgtggcgc agcgcgaggc gcagctccgc ctggcagaag
      121 tccagcgttc ccgcatgacc atccgtgccc ctatcgcggg tttggtgacg cagcgactgg
      181 cgaacgtggg cgaggtcatc ggcctgggcg gcgggtcgat ggtgaccttg accggccccc
      241 tgttcacgct ggtcgacctc gaccgcatgt acgtccgggc cacggtggat gaattcgatg
      301 cccggcagat ccggctgggc caggaggcgc gggtcacgct ggaggcggcg ccgggccgga
      361 cgctgcgtgg acggctctac aagatctcac cggcggtctc gggggagcgc caggaggccc
      421 gcaccttcaa catccgcgtg gccctggagg aggggaagga atttgtgaag cccggcatgt
      481 ctgccgacgt ggaggtgatc gtggccaggc gccgggacgc gctcttcgct cccagccagg
      541 caatcatgga gcgggaaggg aagaagcggg tgtacgtgat cgccgacggg cgggctcgtg
      601 ccaccacggt gaaggtgggg gaaagcaact ggagttccac cgagatcctg gagggcgttc
      661 gcgaggggga tcgggtcatc atcaatccgg acacgcccgg actcacagag ggggccaggg
      721 tgcgggtgga atgagggaca aaaagcgttc ggcagatcgg cggtacggca gttcggcagt
      781 tcggaaggat cgggaacatc cgaacgaccg aacagtcgag cagccgaacg tttttttgtg
      841 tggatcgtga ggcgatgatc gccgttgaag aggtctggcg tacctaccgc atgggaccgg
      901 aacgggtgga cgcccttgcg ggcgtcacgt tccatgtggc gccgggagag ttcgtggctg
      961 tctgtggccc ctcgggctcg ggcaagtcca cgctcatgaa catcctgggc tgtctggacc
     1021 gcgcgacccg cgggcgctac acgctggagg gcgtggaggt gcagtccctg gatgacgacg
     1081 ccttggccgc cctgcggaat cggcggatcg gcttcgtctt tcagacattc aacctgctgc
     1141 cccggatgac cgcgctccgg aacgtggagc ttcccctgat ctacagcggt ctcccgcgcc
     1201 cggagcggca gcaatcggcg ctggaggcca tggcgaccgt cgggctggca gctcggatcg
     1261 ggcaccgccc cagcgagctg tccggcgggg agcagcagcg cggggccatc gcccgcgcct
     1321 tggtgaaccg gccggccatc atcctggcgg atgaaccgac gggaaatctg gacagcaagg
     1381 ccggcgacga gatcctggag gtctttcacc gcctggcgga ggggggcgca accaccatca
     1441 tggtcacgca caacccgcgc atcgccgagc aggcggcgcg ggtcatcctg ctctcagacg
     1501 gccggatcgt ctcggacacc aggagggcag ggacccagag ggcggagccc ctgccggatt
     1561 cgaactccgt cgcggctcct ctaacatctt gagactcggt tccggcgtcc ccggatgacc
     1621 ctatggacct tcgagaagcc ttttccctgg ccgtggagat gctgctggcc aaccgggtcc
     1681 gatccgccct caccacgctg ggggtggtca tcggggtggc cgccatcatc ctgctggtct
     1741 cgctcggcga gggcgctacc gcctacatca cccgcgagat gaccggcctg ggcaccaacc
     1801 tcctgagcgt cactcaaggg aagacgcaga cctccggcgg ttttcatccc cccatggcca
     1861 gcacggtgcg caagctgacg ttcgaggatg cgatggtgct gcgccgccgg gcaacgctcc
     1921 tcaccgatat cgtccccgtg gtgctgggca cagggaaggt ccgtcacctg ggcgccagcc
     1981 gggatatcag cgtcatcggc gtgacacccg agttccagcg ggtgcgtaat ctctacgtgg
     2041 agatcggcca gtttgtgagc gaagaggacg tggaaggccg ccggcgtgtg gtggtgctgg
     2101 ggcggacggt gaagcgagag ctgttcggcg aggagaatcc gctgggccag ttcgtgacgc
     2161 tcgccgactc gcgctaccgc gtgatcggta tcatggagcg gaagggggtg agcctggggt
     2221 tcgacatcga tgacctggtg ttcatcccga cgaagagcgc ccaggacctc ttcgacaccg
     2281 accacttgtt cgagatcctg gcgtcggttc gctcgccgga ggaggtggat caggccatcc
     2341 ggcagatgaa ggacatcctc atgcgccgcc acgggaaccg cgaggatttc acgatcgtca
     2401 gccagggcgc catcctctcg tccttcacca ccatcctgaa gatcctgacc gccgtattgg
     2461 gcgggatcgc cggcatctcc ctgctggtgg gggggatcgg gatcatgaat atcatgctgg
     2521 tctcggtgcg cgagcgcacg cgggagatcg ggatccgtaa ggcggtggga gcccggcgga
     2581 gggacatcct gggccagttc ctgctggagt cggtggtcct cagcagcctg ggcggagggt
     2641 tggggatcct ggtcgggatc ggcggggcca agggcctgtc cttcttcttc acgtatctcc
     2701 cgacgcacgt gtccctctgg gcggtcctta tggctgtggg tttttcggcg gcggtcgggg
     2761 tcttcttcgg cgtgtacccg gcgcgtcggg ccgcctccct ggacccgatc caggccctcc
     2821 ggtacgagtg acatccccga cgccacgccg acgggcggcc agaggatggg aggctggtct
     2881 gatagcagga ggaactgtag tcacggaccc tcgtgcggat gcagggcgtc cggcaggagg
     2941 ctggaggaaa tgagatgact gaacgacgcg cacaccggcc ggccgtcatg gggaggcgcg
     3001 ggatggtggc atctgcccat ccgctggcca gtgcagccgg gctggagatg ttgtggcgag
     3061 gcgggaatgc cgtggacgcg gccgtcgcgg tggcggccac gctgaacgtg gtcgagccct
     3121 tcatgtcggg gattggcggc gacggggtgc tcctgctcac cttgagccgt tcggcggagc
     3181 ggaaggtcct caacttcatg ggccggacgc cgttcgccgc ccgaacggaa gggtgcacac
     3241 cggatgaact tgaccgcggg cccaaggcca gcctggtccc ggggaacttc gcgggctggt
     3301 gggaagccct ccgtgcgcac ggtcgtctgc cgccctcgac ggtcttcgcc ccggccatcc
     3361 gatatgcgga tgagggattt ccgctcacgc atcggggggt cgccttcctg gccgagcatt
     3421 ggcgtcggct gacgccgcag gggggagccg tgttctgtcc gggaggggag gtgccccggc
     3481 ccggcacgtt tctcccgcac caggcgctcg ccgcgacctt gcgacgattg gttgcagagg
     3541 gacccgaccc gctgtaccg
//
LOCUS       DBNGZC010000356         6676 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_127384, whole genome shotgun sequence.
ACCESSION   DBNGZC010000356 DBNGZC010000000
VERSION     DBNGZC010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6676)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6676)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6676
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_127384"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="AC16G0_06415"
     CDS             complement(<1..197)
                     /locus_tag="AC16G0_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099292.1"
                     /translation="MLRYLLTRVVGLAGVLLVVSVVTFLLMHAIPGGPFDEGQMPLSV
                     EARASIARKYSLDKPLHDQYL"
     gene            complement(246..1418)
                     /locus_tag="AC16G0_06420"
     CDS             complement(246..1418)
                     /locus_tag="AC16G0_06420"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - obsolete cysteine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR5099293.1"
                     /translation="MNHVKRAAPDVAAVRRALPVLERVVYLNTGTAGIAAAPVIAKLW
                     EEVSLFEAEGEVRYHEMQDRMDAARAQVASLIGAGADEVAFSRNATDGVNLVAWGLPW
                     KDGDEVVLSDQEHPAMNHPWHHLQSRGGPRLRMFAVDPDPAATLANIRALLTPRTRLI
                     ASSHVSCQTGIRIPAKEVCDLARERGILVFLDGAQAVGQFPVDVKALGCDFYTSNGHK
                     WLHGPKGTGFLYLRRERLDEVCPMQVGAGSFVRPVDLDHIRLMPSAHRYEYGTRSYGT
                     YAALPAAIEYLAGLGWDWVEQRMAHLSGYLKARLSEVPGLRLLTPQPWERSSALVSFS
                     FPNKTAQEMADFLWNASKIRSRTFPDKGILRVSTAYFNTESELDLLVETLQRMANG"
     gene            complement(1510..3267)
                     /locus_tag="AC16G0_06425"
     CDS             complement(1510..3267)
                     /locus_tag="AC16G0_06425"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5099294.1"
                     /translation="MSGSGKDRLSGGVFGRRITRRNLLRGMAAGLGAAGLTAKFGSRS
                     ALAATTGKLVNSVGKEMPPDAAPLGQQVLRMMGRDTKYYDIGYSQYDSSWPQEMMFET
                     LGRLTKDHNAVPGAAERWESSDGKTWRFHLRKDAKWSDGAPVTAHDFEFSFLRRLNPK
                     TAYDFVWFYLDIKNAKAFNTGKIADPKQVGVRALQDYVFEMELEGAVPYWPLIVSFRT
                     SSPCPKHIVEKVGNEWSTNPATLICNGPYKVDKWEKGRRLELVLNPTYTGPHKGFLER
                     FIKPFGEWDAYFPAYEAGESDAIIDIAALSPGNLARAMADPVMSKELHRLTDFLTWYI
                     FFQTAKPPFNDLKVRQAFSHAIDREVLCKTALAGQAVAAYEMLPPGFPSNQAGKLNGI
                     QNFDAAQAKRLLAEAGFPNGKGFPAVDMWLRNPTAQVKTAAEAIQAMYKENLGVTINV
                     KPQEQKIYMDGMRAFNIPMSLISYEYDYADPSNLLGGVWHSQPVGAGRHDWKNDTFDS
                     LVDKAKGLMDQKKRTEMYNEAERILVSDVGGVMVWHPIRVMMWKKYVVGESISPNKAG
                     VSSWYVGNNPVPYIYIAKH"
     gene            3829..4638
                     /locus_tag="AC16G0_06430"
     CDS             3829..4638
                     /locus_tag="AC16G0_06430"
                     /inference="COORDINATES: protein motif:HMM:NF022789.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HIR5099295.1"
                     /translation="MDSIFWYKLALSFVVGSGWVALSTVAAERYGSRIGGLIGGLPST
                     VFVSLLFIGFTQTPMVASETTTVMPVTQGINGIFIIVYLLLAGRGLVASLSGALGVWF
                     ILAGSIAAIGIQHLWVSLCGWALCFCGCYVVVERIMTIPARREMTVQCSVSQAGLRAL
                     FGGAVIALAVLAGKLLGPAYGGIFATFPAMFFATLAITHRAGGAEFSQAVGKAMLTSG
                     MVNVAIYAIAVRYLYLWFGLIQGTALALILACGSGYLTYLFMTTRSASPPP"
     gene            complement(4825..5958)
                     /locus_tag="AC16G0_06435"
     CDS             complement(4825..5958)
                     /locus_tag="AC16G0_06435"
                     /inference="COORDINATES: protein motif:HMM:NF024634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5099296.1"
                     /translation="MSKVYLEVATKLASPLLTGPPLSDELVAFVQHLFTEEEAGVVRH
                     LGQFAGRSAADLAKAEHRPIDQIQPILHRLSVVKRAIAASGPPERQSYRLMPIMPGIF
                     EMVLIGESPEALSPWHRRFAELFEALYETGYIADYQRGQTRPSPFVRVLAVGSTIEAH
                     PMALPSDRLEVILERYTVFGVGQCQCRMTMQVAGHGCRKPVSNCTVMGEFAEQGIRQG
                     WLRPVSRRDVLEIKREAESHGLVTWIMNAEAARGQASCSCCGCCCHAMRMVTEFNAPG
                     VMAPAHFLPRFDTAKCTYCGRCAKTCPMGALVLDVHQHTREHLGARCIGCGLCVVACS
                     DRRAVTMEPVPDYQLPYQSWFAYQLHATAGMVKNAWTVWRHRE"
BASE COUNT         1101 a         2184 c         2149 g         1242 t
ORIGIN      
        1 ctcaggtatt gatcgtggag cggcttgtcc aggctgtact tcctggcgat ggacgcccgc
       61 gcctcgaccg agagcggcat ctggccctcg tcgaagggcc cgccggggat cgcgtgcatc
      121 agcaggaagg tcacgacgga gacgaccagc agcacgccgg ccaggcccac aactcgcgtc
      181 aagaggtagc gcagcatggc tcaaccccgc cgaccgggtg catcaggacg cgccgaccct
      241 tcccctcagc cattcgccat ccgctgcaat gtctcgacca ggaggtccag ctccgattcc
      301 gtattgaagt aggcggtgga gactcgcagg atccccttgt ccgggaaggt gcgcgaccgt
      361 atcttgctcg cgttccacag gaagtcggcc atctcctggg cggtcttgtt cgggaacgaa
      421 aagctgacca gcgcagacga ccgctcccac ggctgcgggg tcaggagccg tagccccggc
      481 acctccgaca ggcgggcctt caggtagccg gagaggtgcg ccatgcgctg ctccacccag
      541 tcccagccca ggcccgccag atactcgatg gcggctggca gcgcggcgta ggtgccgtag
      601 ctccgggtgc cgtactcgta ccggtgggcc gagggcatga gccggatatg gtccaggtcc
      661 accggccgca cgaaggagcc ggcgccgacc tgcatggggc acacctcgtc caggcgctcc
      721 cgccgcaggt ataggaagcc cgtgcccttc ggcccgtgca gccacttgtg gccgttgctg
      781 gtgtagaagt cacagcccag cgccttcacg tccaccggga actgccccac ggcctgcgcc
      841 ccgtccagga agacgaggat gccgcgctcc ctggccaggt cgcacacctc cttggcgggg
      901 atgcggatcc ccgtctggca ggagacgtgg ctgctggcaa tcaggcgcgt gcggggggtg
      961 agcagggccc ggatatttgc cagggtggct gcggggtcgg ggtccacggc aaacatcctc
     1021 agcctggggc cgccgcggga ctggaggtgg tgccagggat ggttcatggc cgggtgctcc
     1081 tggtcggaga ggacgacctc atcgccgtcc ttccacggga gcccccaggc gaccaggttc
     1141 acgccgtcgg tggcgttgcg gctgaaggcc acctcgtccg ccccggcgcc gatcaggctg
     1201 gccacctgcg cccgcgccgc gtccatccga tcctgcatct cgtggtagcg gacctcgccc
     1261 tcggcctcga acagggagac ctcttcccag agcttggcga tcacgggcgc ggcggcgatc
     1321 ccggccgtgc cggtgttgag gtagaccacc cgctccagga ccggaagcgc cctccgcacg
     1381 gcagccacgt caggcgcggc tcgtttcaca tggttcacgc gttcacctcg tcgcggcaga
     1441 gagtacgtca cgccggccag accacgggag tcgggctggc agattgccga agaactcgtc
     1501 ttcagcgcgt cagtgcttgg cgatgtagat gtagggcacg gggttgttgc ccacgtacca
     1561 ggacgagacg ccggccttgt tgggcgagat ggactcgccc acgacgtact tcttccacat
     1621 catgacccgg atcgggtgcc agaccatcac gccgcccacg tcgctgacca gaatccgctc
     1681 ggcctcgttg tacatctccg tgcgcttctt ctgatccatc agccccttgg ccttgtcgac
     1741 gagactgtcg aaggtgtcgt tcttccagtc gtggcggccg gcgccaacgg gctgggagtg
     1801 ccagacgccg ccgagcaggt tgctggggtc ggcgtagtcg tactcgtacg agatgaggct
     1861 catggggata ttgaaggccc gcatcccgtc catgtagatc ttctgctcct ggggcttgac
     1921 attgatggtg acgcccaggt tctccttgta catggcctgg atggcctcgg cggcagtctt
     1981 gacctgcgcc gtggggttgc gcagccacat gtcgaccgcg gggaagccct tgccgttggg
     2041 gaaccccgcc tccgccagca gccgcttggc ctgggccgca tcgaagttct gaatgccgtt
     2101 caacttgccg gcctggttgc tggggaagcc cggcggcagc atttcatagg ccgccactgc
     2161 ctggcccgcc agcgccgtct tgcagaggac ctcgcggtcg atggcgtggc tgaaggcctg
     2221 cctgaccttg agatcgttga aaggcggctt cgcggtctgg aagaagatgt accaggtgag
     2281 gaagtccgtg agcctgtgga gttccttgga catcacgggg tcggccatgg cgcgggcgag
     2341 attgccgggc gacagggcgg cgatgtcgat gatggcgtcg ctctcccccg cctcgtaggc
     2401 tgggaagtag gcgtcccact cgccgaacgg tttgatgaag cgctccagga atcccttgtg
     2461 cggaccggtg taggtcggat tgaggaccag ctcgagtcgc ctgcccttct cccacttgtc
     2521 gaccttgtac gggccgttgc agatcagcgt ggccgggttc gtcgaccact cgttgccgac
     2581 cttctccacg atatgcttgg ggcaggggct ggaggtccgg aaggagacga tcagcggcca
     2641 ataggggacg gccccctcga gctccatctc gaagacgtag tcttgcaggg cgcgaacgcc
     2701 gacctgcttg gggtcggcaa tcttgcccgt gttgaaggcc ttggcgttct tgatgtcgag
     2761 atagaaccag acgaagtcgt aggccgtctt gggattcagg cgccgcagga agctgaactc
     2821 gaagtcgtgg gcggtgacgg gggcgccatc gctccacttg gcatccttgc gcaagtggaa
     2881 gcgccaggtc ttgccgtcgc tcgactccca gcgctcggcc gcgccgggga cggcgttgtg
     2941 gtccttggtg agccgcccca gcgtctcgaa catcatctcc tggggccagc tcgagtcgta
     3001 ttgcgaatag ccgatgtcgt agtacttggt gtcccggccc atcatgcgca gcacttgctg
     3061 accgagcggt gcggcatcgg gcggcatctc cttgcccacg ctgttcacca gcttgccagt
     3121 tgtagccgcc aaggccgagc gagagccgaa cttggccgtc agtccggccg ctcccagacc
     3181 ggccgccatg ccgcgcagca gattgcgccg agtgatccgc cgaccgaaca caccccccga
     3241 cagcctgtct ttgccgctgc cactcatcga gagtcctccc tctttgccac gagtcctgag
     3301 agatcgacac ccccgcgccg ggccgctccc gccggagccg cccggcagtc tacacctgtg
     3361 gattgacagg aatgctcggc ccctcccaag gctttttatg acccagatcc ctgatggaag
     3421 cggaagtgcc gcggagccta ccgccgatgg gcggaagagt caagtaggtc accggtggtg
     3481 tgcaacagaa cctaatcaca tggatgctga gggtgcaatc cgccagctcc tcggatctgc
     3541 aatttgagaa actcatgatc tcatggccgt cccctatccc tcattctcca gacctcgtcc
     3601 ctcgcccttt ccttctctcc agagagaggg cgggggaagg cttgcggggt acgagatcac
     3661 atgtctacat gcattcgcgt tcgtgacggt ctgcaggttc gccaatactc tcggagacag
     3721 gacacaaact acggaacctc ctcgcgtaga cgctggcatt cgcgggtgct tcgcggtagc
     3781 atgcgcggtc ggccgatcag gggaacaggc aagcaatctg aggcacatat ggattcaatc
     3841 ttctggtaca agctcgccct gagtttcgtg gtggggagcg gctgggttgc cctgagtacg
     3901 gtcgccgctg aaagatatgg cagcagaatc ggcggcctga ttggcgggct gccctccacg
     3961 gtctttgtga gcctgctgtt catcggattc acgcaaacac ctatggtcgc ctctgagacg
     4021 acaacggtca tgcccgtgac ccaggggatc aacgggattt tcatcattgt ctacctcctc
     4081 ctcgcgggga gggggctggt cgcgagcttg tcgggcgcac tgggagtttg gttcattctc
     4141 gctggcagca tcgccgccat cggcatccag catctatggg tgtcactctg cgggtgggcc
     4201 ctgtgcttct gtggctgcta cgtggtggtc gagcggatca tgacgatccc tgcccggagg
     4261 gagatgacag ttcagtgcag cgtctctcag gcaggcttgc gggccctctt tggcggcgcc
     4321 gtcatcgccc ttgccgtctt ggcgggtaaa ctgctgggac ctgcgtatgg agggatcttc
     4381 gcgactttcc ctgccatgtt cttcgcgacg ctcgccatta cccatcgagc tggaggggct
     4441 gaattctctc aggcggtggg caaggccatg ctgacgagcg ggatggtcaa cgttgccatc
     4501 tacgcgatcg cggtgcgcta cttatacctt tggttcggtc tcatccaagg gacagcgctc
     4561 gccctcattc tcgcttgcgg cagtggatac ttgacctacc tcttcatgac gacccggtca
     4621 gcctcgcctc cgccgtagtc gcctaccgca caccgggccg gcccgaagtt gtagaaaacc
     4681 acatcccgac cctgacgccg gcccccgaca tacagctcgg cccggtcggc aaactggcgc
     4741 gcactctccc gtgcctggag caacgccgcc aacgctttcg ccgtggcgcg ttgttccggc
     4801 ggacgcagtg gcagcgatgg cgcgtcattc ccggtgccgc cacaccgtcc aggcgttctt
     4861 gaccatgccg gcggtggcgt ggagctggta ggcgaaccag ctctggtagg gcaactggta
     4921 gtcaggcacg ggctccatgg tgacggcccg gcggtcgctg caggccacga cacacaggcc
     4981 acagccgatg cacctcgccc ccaggtgctc gcgcgtgtgt tggtgaacat ccaggaccaa
     5041 ggcgcccatc gggcaggtct tggcgcaccg gccgcagtag gtgcacttgg cggtgtcaaa
     5101 ccggggcagg aagtgcgccg gcgccatgac tccgggggca ttgaactcgg tgaccatccg
     5161 catcgcatgg cagcagcagc cgcagcagga acacgacgcc tgcccccggg ccgcctcggc
     5221 gttcatgatc caggtgacca aaccgtgcga ctcggcctcc cgcttgatct ctagcacgtc
     5281 acgtcgggac accggacgca gccagccctg gcgaatgcct tgctcggcga actcgcccat
     5341 caccgtgcag ttgcttacgg gcttgcggca gccgtgaccg gcgacctgca tggtcatccg
     5401 gcactggcac tggccgaccc cgaagacggt gtagcgctcc aagatgactt ccaggcgatc
     5461 cgacggcaga gccatgggat gggcctcgat cgtgctccca actgcaagca cccgcacgaa
     5521 cggtgacggg cgcgtctgcc cccgttgata gtcggcgatg tagccggtct cgtacagggc
     5581 ttcaaacagc tccgcgaacc ggcggtgcca aggagacaag gcttcgggcg actcgccgat
     5641 gagcaccatc tcgaagatac cgggcatgat cggcatcagg cggtacgatt gcctctccgg
     5701 cgggccgctc gcagcaatcg cgcgcttgac cacggacagc cgatgcagaa tcggctggat
     5761 ctggtcgatc ggccggtgct cggccttggc gaggtccgcg gcgctcctcc ccgcgaattg
     5821 ccccagatgg cggacgactc ccgcctcctc ttcggtgaac aagtgctgca caaatgccac
     5881 cagctcgtcg ctcaggggcg gccccgtcaa cagcgggctc gccagcttgg tcgctacctc
     5941 aaggtagacc tttgacactc cggggaaatc cgtaggcgcc ttcggccgga tagtccttgg
     6001 acggtgtgag gccggtccga cgacgccctg gcgtacttcg atcacgcgcc gcgtggtccg
     6061 ttccgtcatt atgacgctcc tcgccatggt gcaggttcgt tcaatgtcca ctccgggcgg
     6121 gtgcaccggg aaccgtctca gggacacccc caccctgggg cggacctctc acgaggctgc
     6181 ctgccagccc ttcgtggcca catccgctgc atgtcacccc tcaggtctag ccaccagcga
     6241 gcctctgcct gcatggcgtt ctgccgaaga cgggtaaagg ctatcgaatg gtctggtggg
     6301 tgtcaatggc ccaaccctca cccgttctga atcccagtca tcaaacaggc ttgccggcac
     6361 ggcggcagcg ctccccctcg gcatgaccaa ctttcgtccg tcaccgtgtc gagcggctgc
     6421 gctaaggtgg ctttcgcctc gccaagatgg gggcgcgcat ggctgggcac ctggtggccg
     6481 gcggctggaa ggggctagga tacgacctgt cggccacggc cttggacgcc tttgccaggg
     6541 caatccctaa gggagtccga attctcacgg cttgaacttt cgggcttcac cgaatatcct
     6601 cgcccctgta actggcaaca gagtgtggac tctcctaacg gaggcgggtg cgacttcatc
     6661 ccaatgctgt gcgggc
//
LOCUS       DBNGZC010000357         2010 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2573081, whole genome shotgun sequence.
ACCESSION   DBNGZC010000357 DBNGZC010000000
VERSION     DBNGZC010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2573081"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1710)
                     /locus_tag="AC16G0_06440"
     CDS             complement(<1..1710)
                     /locus_tag="AC16G0_06440"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR5099297.1"
                     /translation="MIQIENISKSFGGQVVLRDLTWHITRGQRVGLVGPNGAGKTTLC
                     RILIGEMEVDTGLVRRAKATTIGYLPQEIAPAGDGTVLGHLLAGFPEIQRLEDEMELL
                     AAEMAEAENGGSEDLMHRYGDLQHQYEVLGGYSLEARAKAILGGLGFRPEESHSPLAK
                     LSGGWRMRVSLGRLLLQSPDLLLLDEPTNHLDLESLQWLEDFLAAYEGTVVIISHDRY
                     FLNRVVDRIAELELGRFALYTGDYDDYQAQKLTRQEQIVAAQRTQAEQIEKMERFIRK
                     FRYKATKARQVQSRIKQLDKIERVEVIRAPKRIHFTFPQPLRSGTIVSELRKVRKAYG
                     STLVYAGIDFRLMRGDRVALVGINGAGKSTLLKIVAGVLPFEGGERILGHNVSVHYYA
                     QHQLDALDPKNTVFDELAEVADAETQTRLRTILGAFLFSGDDVEKPVAVISGGEKSRL
                     ALAKMLLRPANLLCLDEPTNHLDVTAREVLEEALDQFDGTMLFISHDRYFINRIATKV
                     VEVRDGRLWEYAGDYDYYLEKKAEDQVAADSRAPSGWQAATTPKDEARAVSPPGPRPS
                     SAGG"
BASE COUNT          339 a          654 c          638 g          379 t
ORIGIN      
        1 gcctccggcc gaggagggcc tcgggcccgg agggctcacg gctcgtgcct cgtccttcgg
       61 tgttgtggct gcctgccacc cagagggtgc ccggctgtcc gctgccacct ggtcttccgc
      121 cttcttctcc aggtagtaat cgtagtcgcc ggcgtactcc cacagccggc cgtctcggac
      181 ctcgacgacc ttggtggcga tccggttgat gaagtagcgg tcgtgcgaga tgaagagcat
      241 ggtgccgtcg aactggtcca gcgcctcctc caagacctcc cgggcggtaa cgtctagatg
      301 gttggtgggc tcatccaggc acaggaggtt cgccgggcgg aggagcatct tggccagagc
      361 cagacggctc ttttcgccgc cggagatgac cgccaccggc ttctccacgt cgtcccccga
      421 gaacaggaag gcgcccagga tcgtccgcag gcgagtctgg gtttcggcgt cggcgacctc
      481 ggccagctca tcgaaaaccg tgttcttcgg atccaatgcg tcgagctgat gctgcgcgta
      541 gtagtgcacc gagacgttgt ggccgagaat gcgctcaccg ccctcgaagg gcaggacccc
      601 cgccacgatc ttgaggagcg tgctcttgcc cgcaccgttg atgccgacca aggccacccg
      661 gtccccgcgc atgagacgga agtcaatacc ggcatagacg agcgtgctcc cgtaagcctt
      721 ccggaccttg cggagttcgc tgacgatcgt tccactccgc agcggctggg ggaaggtgaa
      781 atggatccgc ttgggcgcgc ggatcacctc cacccgctcg atcttgtcca actgcttgat
      841 ccggctctgg acctggcggg ccttggtcgc cttgtatcgg aacttccgga tgaatcgctc
      901 catcttctcg atctgttcgg cctgggtgcg ctgggcggcc acgatctgtt cttggcgggt
      961 gagcttctgc gcctggtaat cgtcgtaatc tcccgtgtag agcgcaaatc gccccagctc
     1021 cagttccgcg atgcgatcca ccacgcgatt caggaagtag cggtcgtggg agatgatcac
     1081 caccgtgcct tcgtaggcgg ccaggaagtc ttccagccac tggagggatt cgaggtccag
     1141 gtggttcgtt ggctcgtcca atagcaggag atcgggggat tgcagcagga ggcggcccaa
     1201 ggatacgcgc atccgccagc cacccgagag cttggccaac ggggaatggg actcctccgg
     1261 ccggaagccc aggcctccca ggatcgcctt ggcccgggcc tccaggctgt atccaccgag
     1321 gacctcgtac tgatgctgca ggtcgccgta gcggtgcatg aggtcctcgg atcccccatt
     1381 ctcggcctcg gccatttcgg cggccaagag ctccatttcg tcttccaggc gctggatttc
     1441 cgggaatccc gccaggaggt ggcccagcac cgtcccgtcg ccggccgggg cgatctcctg
     1501 cggcagatac ccgatggtcg tggccttcgc ccggcgcacc aggcccgtgt ccacctccat
     1561 ttcgccgatg agaatccggc aaagggtggt cttgccggcg ccgttggggc cgaccagccc
     1621 gacccgctgg ccccgcgtga tgtgccacgt caggtcccgc agcacgacct gccccccgaa
     1681 agatttactg atgttctcga tctggatcat tccacccgtc cggagccgtg ttccctcagg
     1741 caggaggagc ggcgttcacg acaccacatt ttgatgatgc gagacctcga cacatgtcat
     1801 gatggaaggg aaggatactc cggaaaaaaa ggcgacgcaa ggttgggtcc gatgtgcggg
     1861 caccagcgtc ggagtcagaa agcccccaca ggcaaatcgg ctccggccgg ccatctcgac
     1921 gttcgagggc cttcttcatg aacgaagcag cgtgggcgga cgtgtggcct tactggacgc
     1981 aaaagacccc gtgttggccc acgcggctca
//
LOCUS       DBNGZC010000358         1556 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2670170, whole genome shotgun sequence.
ACCESSION   DBNGZC010000358 DBNGZC010000000
VERSION     DBNGZC010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2670170"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="AC16G0_06445"
     CDS             <1..412
                     /locus_tag="AC16G0_06445"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR5099298.1"
                     /translation="FLQVVARYVFEISTPWSEELARLCFVWGVFLGAAVGVKRNLHTR
                     VDFVFVRLPRRSAALVLGGMDLLLVVMAIVMIIYGMQLVISTRADSSTSLGYPRNWFY
                     LPVPCSGLLMLRYLIPSVIKRVRGGKGIQGGGEA"
     gene            417..>1556
                     /locus_tag="AC16G0_06450"
     CDS             417..>1556
                     /locus_tag="AC16G0_06450"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR5099299.1"
                     /translation="MLLTLLMLGLVFLGMPISFALGVSSVAFLWQQSVPLVALPQRMA
                     VSVDSFPLLAVPFFVLTGSLMNTGGVTRRIFALANSLVGHVTGGLAHVTVVANMFFAG
                     ISGAAVADAAALGPVMVDAMSRQGFPRAFAAGITAAASTIGPIIPPSIIMVIYAITAG
                     GVSIGALFIGGVIPGILMGLALMALVYAITRRRSYPRNEGFHIPVMLGALREAVLPLL
                     TPAIILGGILGGIFTATESGAVAAVYALILGTLVYREIRFRDLPRILAETMITTAMVI
                     FIVSTAAAFGWIMSYLRVPQLVAGALSAFSRNWWVIFMLLNLLYLFLGCIMEAAAIVI
                     MTIPIIMPILAQVGIDPSHFGVVLAVNMSIGTLTPPVGIVMFVMCQ"
BASE COUNT          217 a          500 c          479 g          360 t
ORIGIN      
        1 gttccttcag gttgtcgccc gatacgtttt cgagatctcg accccttggt ccgaggagtt
       61 ggccaggctg tgcttcgtct ggggggtctt cttgggggca gccgttgggg tcaaacggaa
      121 cctgcacacc cgtgtggact tcgtcttcgt gcgactgccg cgccgctccg ccgccttggt
      181 cctgggggga atggaccttc tgctggtagt catggccatc gtcatgatca tctacgggat
      241 gcaactcgtg atctcgacgc gtgcggattc gagcaccagt ctgggctacc cccgcaactg
      301 gttctacctc cccgtcccct gcagcggcct cctgatgctc cgctacctca tcccaagcgt
      361 gatcaaacgg gtccgcggtg gaaaagggat ccagggcggg ggggaggcgt agccggatgc
      421 tcctcacgct gctgatgctc gggctcgtct tcctggggat gccaatcagc tttgccctgg
      481 gcgtctcctc ggttgctttt ttgtggcagc agagcgtccc cctggttgct ctcccgcaac
      541 gaatggcggt gagtgtggac tcctttccct tgctggccgt gccgttcttt gtcctgaccg
      601 gctccctcat gaatacggga ggagtcacgc gtcggatctt cgccctggcc aacagcctcg
      661 tgggtcatgt cactggcggc cttgcccatg tgacggtggt cgccaacatg tttttcgccg
      721 ggatctcagg cgcggcggtg gcggatgcgg ccgccctggg tccggtgatg gttgacgcca
      781 tgagccggca gggattcccg cgggcctttg ccgcaggaat caccgcagcc gcttccacca
      841 tcggccccat tatccccccc agcatcatca tggtgatcta tgccatcact gccggcggcg
      901 tctccatcgg cgccttgttc atcggtgggg tgatccctgg cattctcatg ggactcgccc
      961 tcatggctct cgtttatgcg atcacgcggc ggcggagcta cccgcggaat gagggctttc
     1021 atattcccgt catgctcggc gcattgcgag aagcggttct gccgctgctg acgcccgcaa
     1081 tcatcctggg gggcatcctg ggagggatct tcaccgccac cgagtcggga gcggtggccg
     1141 cagtctacgc cttgatcctg ggcaccctgg tgtaccgcga gatccgcttc cgcgacctgc
     1201 cgcgcatcct ggccgagacc atgatcacca ccgccatggt catcttcatc gtgtccacgg
     1261 cggcggcctt cggctggatc atgtcctacc tgcgggttcc ccagcttgtg gccggggccc
     1321 tgtcggcgtt cagccggaac tggtgggtca tcttcatgct gctgaacctt ctctacctgt
     1381 tcctgggttg catcatggag gcggccgcca tcgtcatcat gacgatcccg atcatcatgc
     1441 ccatcctggc acaggtcgga atcgacccga gtcacttcgg ggtggtcctg gcggtgaaca
     1501 tgtccattgg gaccctcaca ccgccggtgg ggatcgtgat gtttgtaatg tgtcaa
//
LOCUS       DBNGZC010000359         1664 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_713175, whole genome shotgun sequence.
ACCESSION   DBNGZC010000359 DBNGZC010000000
VERSION     DBNGZC010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_713175"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="AC16G0_06455"
     CDS             complement(<1..1029)
                     /locus_tag="AC16G0_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099300.1"
                     /translation="MKAKSLVRMLVLVAIVGGLVVPCAPALAQTIKIGHEVALTGPNA
                     TWGQSEANAVKMAVEKVNAQGGVLGKKLEIIAYDNRADRLEAVNVAKRLVEQDKVVAI
                     IGPAQSGVANAIREVTNSAKVPVIATTATNPKVTVNDDGSVVPYTFRVCFIDPFQGTV
                     AAQFAYKDLKAKTAAILYDVGDEYSQFLGKYFVEAFEKAGGKITTNEAFRSGELDFRA
                     QLGKIKYGNPDVVFIPTMQKEAALAAKQARDLGIKAKLMGGDGWGSPDLFDLAGPAIE
                     GSYFVNIAALEDPAIQGFIKDYRARYKEDPVLPNPVLAYDAVLWLADAMKRAGTTDGE
                     KLKTAMEQT"
     gene            complement(1349..>1664)
                     /locus_tag="AC16G0_06460"
     CDS             complement(1349..>1664)
                     /locus_tag="AC16G0_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5099301.1"
                     /translation="FNAGFTEIIRRPRSWETSDAWTLLSGFRGRLLLVAGEHDAVIPP
                     GVIRNIYAAARNAAERRLFVAPGASHLILTDLRANAPDQLDHVLGQMTSVLAAAERPR
                     PG"
BASE COUNT          283 a          552 c          507 g          322 t
ORIGIN      
        1 cgtctgctcc atcgccgtct tcagcttctc gccgtcggtc gtgccggcgc gcttcatggc
       61 atcggccagc cagagcaccg cgtcgtaggc gagcacggga ttcggcagca cggggtcctc
      121 cttgtacctg gcgcgatagt ctttgatgaa gccctggatc gccgggtcct ccagggcggc
      181 aatgttgacg aagtaggacc cctcgatcgc cggaccggcc aggtcaaaaa ggtcggggct
      241 cccccagccg tcaccaccca tcagcttcgc cttgatcccc agatcgcggg cctgcttggc
      301 ggcgagcgcc gcttccttct gcatggtggg aataaatacc acgtcgggat tgccatactt
      361 gatcttgccg agctgggcgc gaaagtccag ctcacccgag cggaaggcct cattcgtggt
      421 gatcttgccc ccggccttct cgaaagcctc gacgaaatat ttccccagga actgcgagta
      481 ttcgtcgccc acgtcataca ggatggcggc cgtcttcgcc ttcaaatcct tgtaggcaaa
      541 ttgggcggcg acggtgccct ggaacgggtc gatgaagcag acccggaacg tgtagggaac
      601 gaccgaccca tcgtcattca cggtcacctt ggggttcgtc gccgtggtgg caatgactgg
      661 caccttggct gagttggtca cctcacggat ggcattggcc acgccgctct gcgccggacc
      721 gatgatggcg accaccttgt cctgctcgac caggcgcttg gcgacgttca ccgcctccag
      781 cctgtcagcc cgattgtcat aggcaatgat ctcgagtttt ttccccaaga ccccgccctg
      841 ggcattgacc ttttccacgg ccatcttcac ggcattggcc tcggactgcc cccaggtggc
      901 attcgggccg gtcaaggcga cctcatggcc gatcttgatg gtctgagcca gcgctggtgc
      961 gcaggggact acgagaccgc cgactatcgc caccaggacc aacatgcgaa cgagtgattt
     1021 ggctttcacg gggtttctcc ttctccgctg acgagagctt cgcgagatgg acagcctggc
     1081 gatattcgga caaccggatc gccctgtgct gtgaaacggc ctacggctct tctctcctcc
     1141 gaccttcacc tcctttcaat caccagcccg ccatcaatcc gcggcaggct gtcctagagc
     1201 ccatgacgtg tccgccaccc ctgggcatgg ccgaagtgca gcgactatag ggccagcggc
     1261 ctccctcccg caacggaatt ctcccagggg gtcgtagccg ccgccacccc cccaaggtcc
     1321 ggaggatgcc ccggaagcat atccctgttt atcctggtcg aggcctctcg gcggccgcga
     1381 ggacgctggt catctggccg aggacgtggt cgagttgatc gggggcgttc gcccggaggt
     1441 ctgtgaggat gaggtgggag gcgccggggg ccacgaacag cctgcgctcc gcagcatttc
     1501 gcgccgcggc gtagatgttc cggatcacac cgggcggaat gacggcgtcg tgctcgccgg
     1561 ccacgagcag caggcgtccc ctgaagcccg acaagagggt ccaggcatcc gaggtttccc
     1621 agctccgggg ccgccggatg atctccgtga agccggcatt gaac
//
LOCUS       DBNGZC010000360         3372 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_910562, whole genome shotgun sequence.
ACCESSION   DBNGZC010000360 DBNGZC010000000
VERSION     DBNGZC010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_910562"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(542..1036)
                     /locus_tag="AC16G0_06465"
     CDS             complement(542..1036)
                     /locus_tag="AC16G0_06465"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR5099302.1"
                     /translation="MGFLRKELDRILATAIGLALLAIFFLNMYQVGGRYLFGIGAVWI
                     PDVTRFLFIWMVFLGTALMHLRRGHLVIDFVQVRLPTRLRHATAALISVSMLAMAIIL
                     LVVGWRIMQIRMDIPYTGWEVPTGYAYAAVPVAAVLIGVISLANLYEWLQGRNLRSGP
                     GEAS"
     gene            complement(1234..2514)
                     /locus_tag="AC16G0_06470"
     CDS             complement(1234..2514)
                     /locus_tag="AC16G0_06470"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR5099303.1"
                     /translation="MISILFILLFLFCAIGFEIWAAMGLSAVVYLLIRGDVPLNILAQ
                     SMVAGVDIITLVAIPFFMLAGELMNESGITERLVRFAQYFVGRIQGGLAYVTVIVNVI
                     MAGVSGSAVADASAVSSVLLPAMARDGYDRRFAAAVNAAAATIGPIIPPSIPMVFMAV
                     ISDLSIGRLFLGGVMPGLLMGAFLAVTIYLQSLRHPLPVTSVKGGMAEFLRLSGQALL
                     ALLAPGIVLAGVIFGVVTIVEVAFLAVFYVLLVGIFAYRTIRLRALLHIFSRAAGTSV
                     SILAIFAAVGAYSWIVANEQLGPQVARGIQALGLGRIGFLLVINLFFLFLGCIMDAIP
                     AMLIFFPVLLPVAKALGIDPIHFGVVVVLNLMIGLLTPPVGGLLFLEAKIADIPFDAL
                     VLAVLPYLVALLAVLGLITYVPGLVTFIPNLVFH"
     gene            complement(2584..>3372)
                     /locus_tag="AC16G0_06475"
     CDS             complement(2584..>3372)
                     /locus_tag="AC16G0_06475"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR5099304.1"
                     /translation="QLGGILDMFTGLKAGSIDMVYGGINTYAWIKGGEVYQITSVAFL
                     YRDYDHMRRVLLSPIFKPHLEAAENATGVKVVNINGDTAPRELTTRDKPVRTAADFKG
                     LKIRIAESILARAVFQRLGANPQVIPYADLYMALKQGVVDAQENGVIPVKNMSFYEVQ
                     KYLTLTHYIRDVETFYIVADRWKGLAPAQQKALFDASEEAGNLETELTKQELDEATKF
                     LATRMTVIEPDLPSIRKALEGLYEEQFEGKLWPKGLIQQIREFK"
BASE COUNT          706 a         1029 c         1067 g          570 t
ORIGIN      
        1 ggatggtttt ctggaccaca cggggggagg gttctcgtgg aagtttgagg gcggccggaa
       61 tcgggaagca ggcttgggag aaaagacatc gggcgaggct gtaaaccgct caggcctcgc
      121 ccgtcaaatt gtcttccgcg tgagcggctc ggaaagcggg gccggtcgct cgcggaggaa
      181 ttccccggtt gatgcctgcg gggatcagac cggggcagcc acatgacgcc ggtggcaggt
      241 cattgactac cgtgctagac cgtcaaccac cgtgtggcgc atccagggcg gaggactcct
      301 tgccccctgg gctccagtgt tggtcgatct ccgacgccct gacgcgctgg gattccgtcg
      361 ccgcatgatt cccctcctgg cgaagagccc gaccaacggc cgcgcccctc accttcccga
      421 tggcgacacc cccgttcaga ggcgagcgca atatatctta gatgggattg ggaaagcaag
      481 gaaaaaagat tcacggtgtc ccgtatggga tagttggtgg gtggcccact ggttgcgggg
      541 ctcaggatgc ctctcccgga ccgctgcgca ggtttcgccc ttgcagccac tcgtacaggt
      601 tggcgaggct gatgacgccg atgaggacgg cggcgaccgg gacggcagcg taggcgtatc
      661 ccgtggggac ctcccaaccg gtgtagggga tgtccatgcg gatctgcatg atgcgccatc
      721 ccaccaccag caggatgatc gccatggcca gcatggacac gctgatgagc gctgccgtcg
      781 cgtgccgcag gcgggtcggc aggcgcacct ggacaaagtc aatcacgaga tgtccccgtc
      841 gcaggtgcat caaggctgtc cccaggaaga ccatccagat gaatagaaat cgggtgacgt
      901 ccgggatcca caccgcgccg atgccgaaga ggtaccgtcc acccacctgg tacatgttga
      961 ggaagaagat cgccaggagg gcgagtccaa tcgccgtcgc gaggatccgg tccaattctt
     1021 tgcgcaggaa tcccatcagc cgcctcagac cgagaatttc accgccaagg gcgccaagat
     1081 cgcaaagcca gaccaactct catcaatcca gaaaatcatc aggatcgaag tctcccgggc
     1141 cgttcatccg agtggttccc catcaacgat ttgaattcgg gacgttcctt tgcggtcttt
     1201 acgcgctttg cggttgcgtt gcattatcca ggctcagtga aagacgaggt tggggatgaa
     1261 cgtcaccaag ccagggacat aggtgatgag gccgagcacc gccagaaggg ccacgagata
     1321 cggaagcacg gcgaggacga gcgcatcgaa agggatatcc gcgatcttgg cctcgagaaa
     1381 caggagtccg cccacgggtg gggtcaggag accgatcatc aggttcaaga ccaccacgac
     1441 tccaaaatgg atcgggtcga tgcccagagc cttggcaacc ggcaggagga ccgggaagaa
     1501 gatgagcatg gctgggatgg cgtccatgat gcatccgagg aagagaaaga agaggtttat
     1561 cacgagcagg aatccgatac ggccgagacc gagggcctgg atgccccgcg ccacctgggg
     1621 gccgagttgc tcgttggcga cgatccagga gtatgcgccg accgcggcaa agatcgccag
     1681 gatcgagacc gatgtccctg cggcccgtga gaagatgtgg agcagcgccc gcaggcggat
     1741 ggtccggtag gcgaagatcc ccaccaagag gacatagaac accgccagga acgccacctc
     1801 cacgatggtt accacgccga agatcacccc ggccagaaca atgccagggg ccaggagggc
     1861 caggagcgct tgacccgaga ggcgcaaaaa ctccgccatc ccgcccttga cgctggtcac
     1921 ggggagcggg tgacgcaggg actggaggta gatggtcacg gccaggaacg cgcccatcaa
     1981 gagtcccggc atgacgccgc ccagaaacag ccgaccaatg gacaaatcac tgatgaccgc
     2041 catgaatacc atggggatgc tgggagggat gatgggtcca atggtggccg ccgccgcatt
     2101 cacggcggcg gcgaatcgcc ggtcgtagcc gtcccgcgcc atggccggga gcagcacgga
     2161 ggagaccgcg ctggcatccg ccaccgcgga gccggagacg cccgccatga tcacgttcac
     2221 gatgaccgtg acgtacgcca atccgccctg gatccgaccc acgaagtact gggcgaatcg
     2281 gaccagccgc tccgtgatcc ccgactcgtt catcagttcc ccggccagca tgaagaaggg
     2341 gatggctacc agggtgatga tgtccacccc ggccaccatg ctctgggcca ggatgttcag
     2401 aggcacgtcg ccccggatca gcaggtagac aacggcactc agccccatcg ccgcccagat
     2461 ctcgaagccg atggcgcaga agaggaacag gaggatgaac agaatgctga tcatgccggg
     2521 aagccgccga gggtcagtgc ggggaggcca cctcgaaatg agagcagcct ccccgtctgc
     2581 cggtcacttg aactcgcgga tctgctggat cagtcccttc ggccagagtt tcccctcgaa
     2641 ctgctcctcg tacaggccct ccagggcctt gcggatcgag ggaaggtccg gctcgatcac
     2701 ggtcattctg gtggcaagga acttcgtcgc ctcgtcgagc tcctgctttg tcagctcggt
     2761 ctctaggttc ccggcctctt ccgaggcgtc gaacagcgcc ttctgctggg ccggtgccag
     2821 gcccttccac ctgtccgcca cgatatagaa ggtctcgacg tcgcggatgt aatgcgtcaa
     2881 ggtcagatat ttctggacct catagaacga catgttcttc acaggaatca cgccgttctc
     2941 ctgcgcgtcc accactccct gcttcaatgc catgtagaga tcggcgtaag ggatcacctg
     3001 agggttcgcc cctaatcgct ggaacaccgc tctggccagg atgctctcgg cgatccggat
     3061 cttcagcccc ttgaagtcgg ccgccgtccg gaccggcttg tcacgagtgg tcagctcccg
     3121 gggggcggtg tccccgttga tgttgacgac cttcacaccc gtggcattct ccgccgcctc
     3181 caggtgcggc ttgaagatcg gcgacaggag gacgcgtcgc atgtggtcat aatctcggta
     3241 gaggaaggca accgaggtga tctggtagac ctcgccgccc ttgatccagg cgtaggtgtt
     3301 gatccccccg tacaccatgt cgatgctgcc cgccttcaat ccggtgaaca tgtcaagaat
     3361 gccgcccagt tg
//
LOCUS       DBNGZC010000361         1848 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_518074, whole genome shotgun sequence.
ACCESSION   DBNGZC010000361 DBNGZC010000000
VERSION     DBNGZC010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_518074"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(275..>1848)
                     /locus_tag="AC16G0_06480"
     CDS             complement(275..>1848)
                     /locus_tag="AC16G0_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099305.1"
                     /translation="PVFLVLRASVLTADGVHLTLGNFQKIFSTAFYRAAVINTLEISA
                     GATVAATLLGVPIAFCLARLPVTGKPLAISLGTLPLLLPSFVCAHAWVLLLGRFGFLS
                     EALRAVGIPFGSIYGRGGMIFVYTIQGFPYVLLLAMSAFSAIDVSLEEAGENLGSSPW
                     RTFRTVTLPLLIPAILSGGLLVFMASAENFGVPFVLAEDLPILSLEAYKLFVSEVGGN
                     TPMAGAMGVFLILCTAVPLLLQRAYLRRPHFATQSRGRARMLTVSPRGRRLATALIFG
                     VDFVALLPFLVVIIASFLEMRGPVMFFRFSLQSYREVLTQSSQMITNSYLLATLGTLG
                     AVAFGIPIGYLVTRQHLRTGGFLEVLSNAPLAIAGTVLGIGFVLSFNTHPLILTGGWV
                     ILVLAYTTRMLPFNVRSTVALLHQLDPSMEEASINLGVPPGQTFVRVTVRLLLAGIVS
                     GAILTWVNIASELSSTVILYSGPWATMTVGMFQAMESRNLGMASAFASILVVSVAVPL
                     FFVSRLFGRREVTLF"
BASE COUNT          392 a          585 c          632 g          239 t
ORIGIN      
        1 tctcgatcgc ccgcatgaag ctgtacttga agaagagcgg gtcgcgcggg gggcgggccg
       61 tgaccttctc ggggatgacc ttcacggcga ggaaccaggg gcccggctcc cggagcgccc
      121 gctcgagggc caagccgagg cccatggacg agatcatgcc catggcgccc caggtgtaga
      181 gattccggcc ctgctcgagc cagactcagc caaaggcctc gattgcacca cggagcccgg
      241 catacgccgc atctcctcgc cgaagcgtct tccctcagaa gagtgtcact tcgcggcgac
      301 cgaacagccg cgacacaaag aagagtggta ccgccactga caccaccagg atcgaggcga
      361 aggccgaggc catgcccagg ttccggctct ccatggcctg gaacatgccg acagtcatgg
      421 tcgcccaggg gccgctgtac aggatcaccg tcgagctgag ctccgaggca atgttcaccc
      481 aggtcaggat cgcccccgag acgatcccgg ccaggagcag gcgaaccgtt acccgcacga
      541 acgtctggcc gggcggcacg cccagattga tggaggcctc ctccatggac ggatcgagct
      601 ggtgaagcag cgccaccgtg ctccggacgt tgaagggaag catccgtgtg gtgtaggcca
      661 ggaccaggat gacccagccc cccgtcagga tcagcgggtg ggtattgaag ctcaggacga
      721 acccgatccc caggacggtc ccggcgatgg ccaagggggc gttgctcagc acttccagga
      781 agcctccggt ccggagatgc tggcgggtga ccagatatcc gatggggatg ccgaatgcaa
      841 cggcccccag ggtcccaagc gtggccagga gataggagtt ggtgatcatc tgcgacgact
      901 gggtcaggac ctcccggtag ctctggaggc tgaaccggaa gaacatcacc ggcccccgca
      961 tctccaagaa ggaagcgatg atgactacca ggaacgggag gagcgccacg aagtcgaccc
     1021 cgaagatgag cgccgtggct agtctccgcc cgcggggaga gaccgtgagc atccgtgccc
     1081 gcccccggct ctgggtggcg aaatgcggcc gtcgcagata ggcgcgctgg agcaggaggg
     1141 gcacagccgt gcacaggatc aggaacaccc ccatggcccc cgccattggc gtgttcccgc
     1201 ccacctcgct gacgaagagc ttgtacgcct ccagggagag gatcgggagg tcctcggcca
     1261 ggacgaaggg cacgccgaag ttctcggccg aggccatgaa gaccaggagg ccgcccgaga
     1321 ggatggcagg gatcaatagg ggcagggtca cggtccggaa cgtccgccag ggcgacgatc
     1381 ccaggttttc gcccgcctct tccagcgaca catcgatggc ggagaaggcc gacatagcca
     1441 ggaggaggac ataggggaac ccctggatcg tgtagacaaa gatcatgccc ccccgcccgt
     1501 agatggaccc gaagggaatc cccacggccc gcagggcctc actgaggaac ccgaaccgcc
     1561 ccaagagcag cacccaggcg tgggcgcaca caaacgaggg gagcaggagc ggtagggtcc
     1621 ccaggctgat ggccaggggc tttcccgtca cgggcagacg ggccaggcag aaggcgatgg
     1681 gcacgcccag gagcgtggcg gcgacggtgg ccccggcgga gatctcgagc gtattgatta
     1741 ccgccgcccg atagaaagca gtggagaaga tcttctggaa gttcccgagg gtcaggtgca
     1801 ccccgtcagc cgtgaggacg ctggcccgga ggaccagaaa aaccggca
//
LOCUS       DBNGZC010000362         2418 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1104044, whole genome shotgun sequence.
ACCESSION   DBNGZC010000362 DBNGZC010000000
VERSION     DBNGZC010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1104044"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2148
                     /locus_tag="AC16G0_06485"
     CDS             <1..2148
                     /locus_tag="AC16G0_06485"
                     /inference="COORDINATES: protein motif:HMM:NF047160.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HIR5099306.1"
                     /translation="TTMRPGFLKQARALAAITLILSQLWVASGAWAQTGPPFNPTNMP
                     FDMTGFIQAATLDPTCPADPLCGGTITVNDQVITVPKNTILQMPAAALTWQQVFAQAP
                     AAYKALGQSGLALSDGAPLAVPPVLAPLGTYEVHVQGNRVGAAYIAGLLFLSQSSLQS
                     SQGFINWIDLTTGMFEVGGTIGVAGTGQRVTINDPLGRFGRASDPTVIDARFTIDENN
                     PTVRSATGYPMCIPRSAADGLCPQTNRPLDATSPSGFKMIFTYPAVASVVAGGPDPRL
                     MAPFEIGDYVTYSGIMVADPAPATTTYVAAYQVIGNVGLYTAHGDSLAYVATDVMLLG
                     TGGNQVLGLAEATVRTRFEGFTTDPSRNILLFGVDVNACNGTATPRSWGAIGVDQGPP
                     TGAVLGRWRFRPPCSAVGTPTDKACVMAPAGTFLPPTREMRASIETVKGSGIPAPTVV
                     TANGLTVNQYQAPIFDFLFPENTMIGSPVVPLNLDTMPFLAQGSGPLDTTNPLSPIVG
                     QLNLWPGLPVPTAANCAIAGVPLANAGTAQTVASGATVTLNGSATGGLAPYTFAWTQT
                     AGPTVILTNANTANPIFTAPLVPAGSSATLTFSLLVTDSAGVASAASSVTITVNPVVS
                     DVVTISLVQYRISKQRLTVNAGSTASTAVPPANLTVQAFDANGVAQGPPQPMVLTAGV
                     YILDVLGAPQPAKVVVTSDHGGSATSPLTSLRQ"
BASE COUNT          403 a          888 c          739 g          388 t
ORIGIN      
        1 acgacgatga gacctggatt cctgaagcaa gcacgtgccc tcgcggccat cacgctcatc
       61 ctgagccagc tctgggtggc cagtggcgcc tgggcccaga ccggcccccc cttcaatccg
      121 accaacatgc ccttcgacat gaccggcttc attcaggcgg ccaccctgga tccgacgtgt
      181 ccggccgacc cgctctgcgg gggcacgatc accgtgaacg atcaggtgat caccgtgccg
      241 aagaacacga tcctgcagat gccggcggcg gccctcacct ggcagcaggt gttcgcccag
      301 gccccggcag cttacaaggc cctgggtcaa agcgggctcg cgctgagcga cggcgccccc
      361 ctggccgtgc ccccggtgtt ggcccccctg ggaacctatg aggttcatgt ccagggcaac
      421 cgcgtcgggg ctgcctacat tgcgggcctg ctgttccttt cgcaaagctc cctccagtcc
      481 agccagggtt tcatcaactg gatcgacctc accacgggaa tgttcgaggt cgggggcacc
      541 atcggcgtcg cgggcaccgg ccagcgggtg acgatcaatg atcccctcgg acggttcggc
      601 cgggcgtccg accccacggt gatcgacgcc cgcttcacca tcgatgagaa caatccgacc
      661 gtccggagcg ccacgggcta ccccatgtgt attccccgga gcgcggcgga tggcctctgc
      721 ccgcagacca accggcccct ggatgcaacc tcacccagcg gcttcaagat gatcttcacg
      781 tacccagcgg tggcgagcgt cgtggcgggc ggcccggatc cccggctcat ggccccgttc
      841 gaaatcgggg actacgtcac ctacagtggc atcatggtgg ccgacccggc cccggcgacc
      901 accacctatg tcgccgccta ccaggtgatc gggaacgtcg gcctctacac cgcccacggg
      961 gatagcctgg cctatgtggc cacggatgtc atgctcctgg gcacgggcgg caaccaggtg
     1021 ctggggctgg ccgaggccac ggtccgcacc cgcttcgagg gcttcaccac cgatcccagc
     1081 cgtaacatcc tgctctttgg cgtcgacgtg aacgcgtgca acgggactgc gactcccagg
     1141 tcctgggggg ccattggtgt ggatcagggg ccgcccaccg gcgccgtcct gggccgctgg
     1201 cgcttccggc ccccctgcag cgcggtcggc accccgacgg acaaggcctg cgtcatggcc
     1261 cccgccggca ccttcctgcc gcccacccgc gagatgcggg ccagcatcga gacggtgaag
     1321 ggctccggca tcccggcgcc gacggtagtg acggcaaacg ggctgaccgt caaccagtac
     1381 caggccccca tcttcgactt cctgttcccg gagaatacga tgatcggatc cccggtggtg
     1441 ccgctcaacc tcgacaccat gccgttcttg gcccagggtt ccgggccgct ggatacgacc
     1501 aacccgctga gcccgatcgt gggccagctc aatctctggc caggcctccc ggtcccgacc
     1561 gcggcgaact gcgccattgc gggtgtgccg ttggccaacg cgggcaccgc gcagaccgta
     1621 gcctcggggg cgacagtcac cctcaacggc agtgccaccg gtggcctggc gccgtacacc
     1681 ttcgcctgga cccagactgc cggccccacg gtgatcctca ccaacgcgaa tacggccaac
     1741 ccgatcttca ccgccccgct cgtccccgcg ggcagttcgg caacgctgac cttcagcctc
     1801 ctcgtcacgg atagtgccgg cgtggcctcg gccgcctcgt cggtcacgat cactgtcaac
     1861 cctgtggtat cggacgtggt caccatcagt ctggtgcaat accggatcag caagcagcgg
     1921 ctcacggtga atgcgggctc gaccgcctcc accgcggtcc cgccggccaa tttgacagtg
     1981 caggcctttg acgccaacgg cgtcgcccag ggccccccgc agcccatggt cctcacagcg
     2041 ggggtctaca tcctggatgt tctcggggcg ccacagccgg ccaaggtggt ggtaacctcc
     2101 gaccacggcg gctcggccac ctcgcccctc acgagtctcc ggcagtagcc gtatcccggc
     2161 cgggcgagga gccagtttcg aggactcctc gcccgggcca ggggcggcaa ggaacacatg
     2221 caggcttcgt tactcactga actgattcca gcgccgcaga cacatcccac gctgcagcgc
     2281 tcacacgatc cccgtgatgc tcgacacggg aaaaggagac cgatcatgac accgagatgg
     2341 agaaacagcg tcctatctct ctgggctgtg gccctgattc tggtccagct cttgctagcc
     2401 accggggtta gatcggaa
//
LOCUS       DBNGZC010000363         2425 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2475803, whole genome shotgun sequence.
ACCESSION   DBNGZC010000363 DBNGZC010000000
VERSION     DBNGZC010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2475803"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="AC16G0_06490"
     CDS             <1..909
                     /locus_tag="AC16G0_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5099307.1"
                     /translation="TRIVGRGDKVAGLETIQVRRVFDEARRFSPQFIEGTKQVMDADS
                     VILAIGQAADLEFLTPKDGVEVTPRGTIKVDPETLGTTARGIYAGGDVAFGPRIFIEG
                     VANGHRAARSMHEYLSGQRLAISAETGWVRLDHAAQALLGPIAEGPRLPRVYPGYRAA
                     GRQEPPGLPIDRRIGIAEVELKYPDEAARTQARRCVRCGIHTIFDATRCILCGGCVDV
                     CPEYCLRMVPINQVAGEDPVTSLITAVGGAALSPTPGPQRPTPTATVMIMDATKCIRC
                     ALCAIRCPTGAISMEAFRYAETWVPV"
     gene            884..1396
                     /locus_tag="AC16G0_06495"
     CDS             884..1396
                     /locus_tag="AC16G0_06495"
                     /EC_number="7.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="QcrA and Rieske domain-containing protein"
                     /protein_id="HIR5099308.1"
                     /translation="MPKPGFLSETPTPDGSRVSRSLLLGTGWCGFVLSLVGPALANIR
                     FLFPNVVYEGPTLFKVGRPEDYPPGSVTFLEDQRLYIVRDPTGFRALSAVCTHLRCTV
                     GSFVPPDTQWKETHARCPCHGSIFAKDGRVLQGPAPRPLDFYRVSLSPDGRLLVDTAS
                     IVSKDDLFKA"
     gene            1424..>2425
                     /locus_tag="AC16G0_06500"
     CDS             1424..>2425
                     /locus_tag="AC16G0_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HIR5099309.1"
                     /translation="MADESGTEVAVEQGIQERVRRSVFRHDYPQTDLDRMSTMVTNFF
                     LHLLPAKVHPNSLRLTYPWALGMIAFFLMAILFVTGFLLMFFYIPSTDWAYHSMKDLQ
                     FVVSFGPLLRNIHRWSAHGMVAVVLLHMCRVFYTASYRRPREFNWILGVVLWVVTLFL
                     SFTGYLLPWDQLAFWAITVGTSIASYPPWIGDTIRVLMLGGNTVGQGALLRFYVLHVA
                     VLPAALGLLVAIHFWRIRKDGGLSRPNGTASVPANGRPFAPGPIKTYALMEIVRGTSP
                     MVGAQAPEEEVPSWPHLLFRLVVLFQVVLAVVMLLGIFFGAPLEELANLTHPPNPAKA
                     "
BASE COUNT          379 a          817 c          769 g          460 t
ORIGIN      
        1 acacgcatcg tgggccgggg ggacaaggtt gccggtctgg agaccatcca ggtccgccgg
       61 gtgttcgatg aggcgcggcg gttcagcccc cagttcatcg aagggaccaa gcaggtgatg
      121 gacgccgatt cggtgatcct ggccatcgga caggcggcgg acctggagtt tctcaccccg
      181 aaggatggcg tggaagtcac gccccgggga accatcaaag tggatccgga gactctgggc
      241 accactgccc gcgggatcta cgcgggggga gacgtggcct tcggcccccg gatcttcatc
      301 gagggcgtgg ccaacgggca ccgggccgcc cgctccatgc acgaatatct gagcggccag
      361 cgtctggcca tctcggcgga gacggggtgg gtgcggctgg atcatgcggc gcaggcactc
      421 ctggggccaa tcgccgaggg gccacgcctt cctcgggtct atcccggata ccgtgcagca
      481 gggcggcagg aaccaccggg tcttcccatc gaccggcgga tcggcatcgc ggaggtggag
      541 ttgaaatacc cggacgaagc ggcccggacc caggcccgcc gatgcgtccg gtgcgggatc
      601 cacaccatct tcgatgccac ccgctgcatc ctttgcgggg gctgcgtgga cgtgtgcccc
      661 gagtactgtt tgcgcatggt gcccatcaat caagtggcgg gggaggatcc ggtgacatcc
      721 ctgatcaccg ctgttggcgg cgccgctctc tctccgaccc ccggtcccca acgcccgact
      781 cccactgcca cagtgatgat catggacgcc acgaagtgca tccggtgcgc cctgtgtgcc
      841 atccgctgcc ccacgggcgc catcagcatg gaggccttcc gctatgccga aacctgggtt
      901 cctgtctgaa acgccgacgc cggacggatc gcgagtttcc cgcagcctgc tgctcggcac
      961 gggttggtgc gggttcgtcc tctccctcgt ggggccggcc ctggccaaca tccggtttct
     1021 ctttcccaac gttgtctacg agggccccac gctcttcaag gtcggccggc ccgaggatta
     1081 cccccccggc tcggtgacgt tcctggagga ccagcggctc tatatcgtgc gcgacccgac
     1141 cgggttccgg gccttgtcgg cggtctgcac acacctgcgc tgcaccgtcg gctcattcgt
     1201 gccacccgac acgcagtgga aggagaccca cgcccgctgt ccgtgccacg gcagcatctt
     1261 cgccaaggac ggtcgggtcc tccaggggcc cgcgccacgc cccctggact tctatcgggt
     1321 gagtctctcg ccggatgggc ggctcttggt ggacacggcg agcatcgtga gcaaagacga
     1381 cctcttcaaa gcgtgacgca gacaacacaa agggaagcca gacatggcag acgaatccgg
     1441 gacggaggtg gcggtggagc agggcataca agagcgggtc cggcggtccg tcttccggca
     1501 cgactaccct cagacagacc tggaccgtat gtccaccatg gtcaccaact tcttcctgca
     1561 ccttctgccg gccaaggtgc acccgaacag cctgcgcctc acctacccct gggccctggg
     1621 gatgattgcc ttctttctca tggccatcct gttcgtcaca ggcttcctgc tcatgttctt
     1681 ctacattccg tccacggact gggcctacca cagcatgaag gatctgcagt tcgtggtgtc
     1741 cttcggccca ctcctccgga acatccaccg ctggtcggct cacgggatgg tggccgtggt
     1801 gctcctgcac atgtgccggg tcttctatac ggcttcgtac cggagaccac gcgagttcaa
     1861 ctggatcctg ggggtggtgc tctgggtggt gaccttgttc ctgagcttca ccgggtactt
     1921 gctcccctgg gatcagctgg ccttctgggc catcaccgtg ggcacgagca tcgccagcta
     1981 ccctccgtgg atcggggaca cgatccgcgt cctcatgctc ggcggcaata ccgttgggca
     2041 gggggccctg ctccgcttct acgttctgca tgtcgcagtc cttcccgcag cccttggact
     2101 cttggtcgcg atccatttct ggcggatccg aaaggatggc ggcctgtcgc gccccaacgg
     2161 gacggcgagt gtcccggcca acggtcgccc ctttgcgcct ggacccatca agacttacgc
     2221 gttgatggaa atcgtgcgag ggacttcccc catggtgggg gcgcaggcgc cggaggaaga
     2281 ggtgccgtcg tggccccacc tcctgttccg gctggtggtc ctcttccagg ttgttctggc
     2341 cgtggtcatg ctcttgggaa tcttcttcgg tgcgcccctg gaggaacttg ccaacctcac
     2401 gcatcccccg aatccggcca aggcc
//
LOCUS       DBNGZC010000364         2759 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_812589, whole genome shotgun sequence.
ACCESSION   DBNGZC010000364 DBNGZC010000000
VERSION     DBNGZC010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_812589"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1166)
                     /locus_tag="AC16G0_06505"
     CDS             complement(171..1166)
                     /locus_tag="AC16G0_06505"
                     /inference="COORDINATES: protein motif:HMM:NF024869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR5099310.1"
                     /translation="MRPDILELAGDLVKRGDPFALAIVVRREPPTSAQVGDMALVTLA
                     GAFHGWLGGSCTQPTVVREAQRALADGMPRLIALSPDPGADHRPGITPIPATCASGGS
                     VDIYIEPVLASPRLVILGASAAAQALARLGKAMGYSVDLADPQLDPATFPQTDRVFTD
                     PEAPDLRQPFPHPARLSVIVATMGQWDELATSIALALEPSYLGVVASRKRFEQICETL
                     VARGVSPDTLARIKNPAGLDIGAETPEEIALSILSEIVQVHRRASKEGSAARPEALQA
                     AEELDPVCGMTVAVASAVHLAEVGGRTYYFCCGGCRERFLAAPGRYAAGAGMGAA"
     gene            complement(1156..1785)
                     /locus_tag="AC16G0_06510"
     CDS             complement(1156..1785)
                     /locus_tag="AC16G0_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR5099311.1"
                     /translation="MRPERADAVAGIVLAAGASQRMGRNKLFLELEGETLLRRTVRRA
                     GAAGLSPVLVVLGYQAERGLREVTGIPCRPILNPDYGRGINVSLRAGIAAVPAEARAA
                     VLVLGDMPFVTARMLATLVERFREGRAPLVISEYAGVQAPPTLFDRSLLDEFLMMEED
                     ACAKRLVKRHRSQALVVSWPGEILTDLDLPEDYERVKTLTTAGRNPGAP"
     gene            complement(1775..>2759)
                     /locus_tag="AC16G0_06515"
     CDS             complement(1775..>2759)
                     /locus_tag="AC16G0_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR5099312.1"
                     /translation="SYRRPGAKFLVEEGGQTVGGVSGGCLEADVREVALAVIRSGVPR
                     LLHYDTGDDEQRVWGLGLGCNGSVDIFVQLSTSEAALQVAGQVKKLLEGEDAFAVSTL
                     VEGPGQVGSALVLGRAGVLDGSLGDSHLDSQTVGLALDQLTRGESALHAVGAGRVFTE
                     VYTPPPHLIICGAGDDSIPLVGYAAAAGFRVTVVDHRPAYLTPERFPAATRLLRLRPE
                     EDLSPLPLGPRSYVVLKAHSLAHDREWARRLLLTEVPYIGLLGPWKRKDQILDQIGSS
                     GSPRVFGPVGLDLGAEGPEQIALSSVAELLAVRSGREPNHLRTREGAIHAA"
BASE COUNT          453 a          955 c          935 g          416 t
ORIGIN      
        1 gagaaggcgc gccagcacct tggcgatctc ggtcttgccg acccccgcgt ctccttcgac
       61 caggagaggc ttctgcatct ccttggcgag gaagacggcc aatgcgatct ccggcccggc
      121 gatgtaaccg taccgctcca tggcttcctg catggcgcgg atctcggtcc tcatgcggct
      181 cccattcctg cgcccgccgc atagcgcccc ggcgctgcca ggaaccgctc gcgacagccc
      241 ccgcagcaga agtagtaggt gcggccgccg acttcggcga gatggacggc ggaggcgacg
      301 gccacggtca tgccgcacac cgggtccagc tcctccgcgg cctgcagggc ctcgggtctc
      361 gccgccgatc cctccttgga agccctgcgg tgaacctgta caatctcgga caagatactg
      421 agggcgatct cctcgggtgt ctcggccccg atgtcgagcc ccgccggatt cttgatccgc
      481 gccagggtat ccggggagac gccccgagcc accagtgtct cgcagatctg ctcgaatcgc
      541 ttccggctgg ccacgacgcc gaggtatgag ggctccagcg ccagcgcgat ggaggtggcc
      601 agttcatccc actggcccat ggtggcgacg ataacggaga ggcgggcagg atgggggaag
      661 ggttgccgaa ggtccggagc ctccgggtcg gtgaagaccc tgtccgtctg tgggaaggtg
      721 gccgggtcga gctgcggatc ggccaggtcc acgctgtatc ccatcgcctt gcccaggcgg
      781 gcgagagcct gggctgccgc cgacgcaccc agaatgacca gacgagggga tgccaggacc
      841 ggctcaatgt agatatccac actgcctccg ctggcgcacg ttgccgggat gggagtgatt
      901 ccggggcgat ggtccgcgcc gggatcgggc gaaagtgcga tcagccgggg catcccgtcc
      961 gccagagccc gctgggcctc gcgcaccacc gtcggctgcg tacagctccc gcccagccag
     1021 ccgtgaaacg cgcccgcaag cgtgaccagt gccatgtccc ccacctgggc tgaggtcggc
     1081 ggctcgcgac ggacgacaat cgccagggcg aacggatcgc cccgcttgac gagatcgccg
     1141 gccagctcca ggatgtcagg gcgcacccgg gtttctccca gccgtggtca atgtcttgac
     1201 ccgctcgtaa tcctcgggca gatcgaggtc agtgaggatc tctcccggcc aggaaacgac
     1261 gagcgcctgg ctgcggtggc gcttcacgag acgcttggcg caggcgtcct cttccatcat
     1321 gaggaactcg tcgaggagcg atctatcgaa gagcgtgggt ggggcctgca cgccggcgta
     1381 ctctgagatc acgagggggg cccgaccctc ccggaagcgc tcgacgagcg tcgccagcat
     1441 gcgggccgtg acgaatggca tgtccccgag gacgagcacc gccgctcgtg cctccgcggg
     1501 aaccgcggcg atgccggcac ggagcgagac gttgatgccc cggccgtagt ccgggttcag
     1561 gatcggtcgg caggggatgc ccgtcacttc tcgcaggccc cgctcggcct gatatcccag
     1621 gacgacaaga accggtgaaa gaccggccgc ccctgctcga cgcacggtcc gccggaggag
     1681 cgtttcgcct tcgagctcca ggaaaagctt gttccggccc atgcgctgcg aggcccccgc
     1741 ggccagcaca atccccgcca cagcgtcggc ccgctcaggc cgcatggatc gcgccctccc
     1801 tggttcggag gtgatttggc tcccggcccg accgaacggc cagcagttct gcgacgctgc
     1861 tcagggcgat ctgctcggga ccctcggcgc cgagatcgag gcccacgggc ccgaagaccc
     1921 gagggcttcc ggacgatccg atctggtcga ggatctggtc cttgcgcttc cagggcccca
     1981 ggagtccgat gtaagggacc tcggtgagga gcaggcgcct ggcccattcc ctgtcatggg
     2041 ccagcgagtg cgccttcagc acgacgtagg agcgtggccc gaggggaaga gggctcaggt
     2101 cctcttcggg tctcaggcgg agcaggcgcg tggcggcagg gaaccgctcc ggcgtgaggt
     2161 aggcaggacg gtggtccacc acggtgacgc gaaaccccgc ggccgccgca tagccaacca
     2221 ggggaatcga gtcgtcgccg gcaccgcaga tgatcaggtg ggggggcggg gtgtacacct
     2281 ccgtgaacac ccggcccgcc cccactgcgt ggagggccga ctcgccccgt gtcaattgat
     2341 cgagggcgag acctacggtc tggctgtcca gatgggagtc gcccaaagag ccgtcgagca
     2401 cgccggcacg ccccaggacc agcgcagagc ccacctgtcc ggggccctcc acgagcgtgg
     2461 acacggcgaa tgcgtcctct ccttccagga gtttcttcac ctgccccgcc acctggagcg
     2521 cagcctcaga ggtcgagagc tgtacgaaaa tgtccaccga gccattgcag cccagcccaa
     2581 gtccccaaac gcgctgctcg tcgtcgccgg tatcgtagtg caggaggcgc gggacgccac
     2641 tccgtatgac cgccagggcg acttcgcgga cgtctgcttc gaggcacccg ccgctcacgc
     2701 ctcccaccgt ctggccgcct tcctcgacga ggaactttgc gccgggccgc cggtaggag
//
LOCUS       DBNGZC010000365         2947 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2280815, whole genome shotgun sequence.
ACCESSION   DBNGZC010000365 DBNGZC010000000
VERSION     DBNGZC010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2947)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2280815"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1926
                     /locus_tag="AC16G0_06520"
     CDS             <1..1926
                     /locus_tag="AC16G0_06520"
                     /inference="COORDINATES: protein motif:HMM:NF013713.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HIR5099313.1"
                     /translation="IGLAVVMNAAVGVLLVSLDSRVGIGRRIVAAAGMLVLSAAALLG
                     FRTWDKGIMTSGVTIYASNYKSLPSDALRREWMTRDELLYYREGLTATISVHRSYGAD
                     YIYEKTNGKVDASYGDTPTQLMVGYLPMLFNPGAKRVLVIGMGSGMTAKAVAAFPVTR
                     LEVAEIEPAVIEGARFFAEKNGRIHDDPRVRFIHADGRNYLLAVQERYDAIISEPSNP
                     WIAGVGNLFTREYYTLAREKLADGGVFGQWMQTYAMAPEDLRMVYRTFAEVFPDVSLW
                     AVNDSDMLLIGTLRPQRLRYTDLTRALADRPVAQRDLRGLGIRNPYSLLAMYLMPKAA
                     LLKMAGDAEINLDDVPRLEYSAPRNLGRDTVDLNSSLTKKFIVRPVVEDADPERDASG
                     RLALYLAYGYKAVRDRGQALTWVERGLARNPMDAEARMLHARLLADDGRSAEAAEELR
                     KVVAGPAALLGEVAEVAKSLDSDDAIPILRRVRQRDPRLLLARVALADALLQGGHPRQ
                     AESEYRELQPAHPSDPRILLGLGKALLVESDYAPALAALDGAAKLGETSAELQSERGE
                     TLMWLGRYREAAMAYRQALRGNVEEVTWRLNLGISLAQLGPAYTGEAEQRFREVLAMD
                     PDNTRAWEELHNLGKRF"
     gene            1992..2903
                     /locus_tag="AC16G0_06525"
     CDS             1992..2903
                     /locus_tag="AC16G0_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099314.1"
                     /translation="MVQPMEDYLRDRLTVEARTPDFLRARVSFPPDLQGPPEAGHGGG
                     ITAMLFELVRLLEGERWDGIRVPRPVRVEVALQRELPLDTALQAESERTPGGWHSRIL
                     QEGRPIAEAEVRSMEGPLPPPPAEVRRDWEASQAKAYELPGYEFCLGCGFSNPRGAQI
                     RFDFNELMVWKRLTPQTHFRSAKGGLFPGYLCIVCDELGWWLGALRQGECGLSNRVVV
                     CLGESIAADVPLLAVGARSAVVALDKKGRVWRTLVSIVTPEWSPVATAEVQFVGSRAF
                     TKTMLSRFLPGNDPSAIRRVFPKYDSC"
BASE COUNT          474 a          924 c         1072 g          477 t
ORIGIN      
        1 atcggcctgg cggtcgtcat gaacgcggcg gtgggcgtct tactggtatc gctggactcc
       61 cgggtgggga tcgggcggcg gattgtcgcc gcggccggga tgctggtcct ctcggcggcg
      121 gccctgctgg gattccggac gtgggacaag gggatcatga ccagcggggt gaccatctac
      181 gcatcgaact acaagagcct gcccagcgac gcgttgcgac gcgagtggat gacgcgggat
      241 gaactgctct actaccggga gggcctgacc gccaccatca gcgtgcaccg ctcctatgga
      301 gccgactata tctacgagaa gaccaacggg aaggtggatg cttcctacgg cgacacaccc
      361 acccagctca tggtggggta tctccccatg ctgttcaatc ccggcgccaa gcgggtgctg
      421 gtcatcggga tggggagcgg gatgacggcc aaggcggttg ccgccttccc ggtgacgcgg
      481 ctggaggtgg cggagatcga gccggccgtg atcgagggcg cgcggttctt cgccgagaag
      541 aacggccgga tccacgacga tccccgggtt cgattcatcc acgccgacgg gcgcaactac
      601 ctgctggcgg tccaggagcg ctatgacgcc atcatctcgg aaccatccaa cccgtggatc
      661 gcgggtgtcg gcaacctgtt cacccgggag tactacaccc tggcgcggga gaaactggcg
      721 gacggggggg tcttcggaca gtggatgcag acttacgcca tggcccccga agacctccgg
      781 atggtttacc ggaccttcgc cgaggtgttc cccgacgtgt cgctgtgggc ggtcaacgac
      841 agcgacatgc tgctgatcgg aaccctccgg ccgcaacggc ttcgctacac cgaccttacg
      901 cgggccctgg cggaccggcc cgtggcccag cgggatctcc ggggcctggg cattcggaat
      961 ccatacagcc tgctggccat gtacttgatg cccaaggcgg ccctgctgaa gatggcgggt
     1021 gacgcggaga tcaatctgga cgacgtcccc cgcctggagt attcggcacc acgcaatctg
     1081 ggccgggaca cggtggacct gaacagcagc ctcaccaaga agtttatcgt ccgcccggtg
     1141 gtggaggacg ccgatccgga gcgggacgcc agcgggcgcc tggccctgta cctggcgtac
     1201 gggtacaagg cagtccggga ccgagggcag gccttgacct gggttgaacg tgggctcgcg
     1261 agaaacccga tggatgccga ggcgcggatg ctgcatgcca ggctcctcgc ggacgacggg
     1321 cgatcggcgg aggctgccga ggagctccgg aaggtcgtgg cggggccggc cgccctcctg
     1381 ggggaggtcg cggaggtggc gaagtcgctg gattccgacg acgcgatccc gatcctgcgg
     1441 cgggtccgcc agcgagaccc ccggttgctc ctggcgcgcg tggcgctggc cgacgccctg
     1501 ctgcaggggg ggcatccccg ccaggcggaa agcgagtacc gcgaactcca gccggctcac
     1561 ccgagcgatc ctcgtatcct cttgggcctg gggaaagcgc tcctggtcga gtcggattac
     1621 gctcctgccc tcgcggcctt agatggggcg gcgaagctcg gcgagaccag cgcagagctt
     1681 caatccgagc gcggcgagac gctgatgtgg ctcgggcgct accgggaggc ggccatggcc
     1741 taccgccagg cgctccgagg gaatgtggag gaggtgacct ggcgcctgaa tctggggatc
     1801 tcgctggcgc aactcggacc ggcctacacg ggcgaggcgg aacagcgatt ccgcgaggtc
     1861 ctggccatgg acccggacaa cacccgcgcc tgggaagagc tccacaacct cggaaagcgc
     1921 ttctgaggac aatggtcggg gccggggagc cggcgtggca acgtccaggg gggataggcg
     1981 gagacgtcgt gatggtccaa ccgatggaag actatctccg agatcgcctg acggtggagg
     2041 cgcggacgcc cgatttcctc cgggcccgcg tgagtttccc tcccgacctt cagggaccgc
     2101 cggaggccgg acatggcgga ggcatcaccg ccatgctctt cgagctggtg cggctgctcg
     2161 agggggagcg gtgggacggg attcgcgtcc cccgaccggt gcgggtggag gtggcgctcc
     2221 agcgtgagct cccgctggat acggcgctgc aggcggagtc ggagcggacg ccgggcggct
     2281 ggcacagtcg gatccttcag gagggacggc ccatcgcgga ggccgaggtg cggtccatgg
     2341 aggggccatt gccgccgccg cccgccgagg tccggcggga ttgggaggct tcccaggcga
     2401 aggcgtacga gcttccaggg tacgaattct gtctcgggtg cggcttctcc aatccccgtg
     2461 gcgcccagat ccggttcgat ttcaacgagc tgatggtgtg gaagcgcttg accccccaga
     2521 cccacttccg ctctgcgaaa ggcggcctgt tcccggggta cctgtgcatc gtctgtgacg
     2581 aacttggctg gtggctcggg gccctccgcc agggcgagtg cggcctctcg aatcgcgtcg
     2641 tggtgtgtct gggagaatcg atcgcggccg acgttcccct cttggcggtg ggcgcgcggt
     2701 cggccgtggt ggcgctggac aagaagggca gggtgtggcg gacgctggtg tcgatcgtca
     2761 cgccggagtg gagcccggtg gccacggccg aggtgcagtt cgtgggcagc cgggccttca
     2821 ccaagaccat gctgtcgcgc ttcctccccg ggaacgatcc gtccgccatt cgtcgggtct
     2881 ttcccaaata cgacagttgc tagaccactg ctccccatct gacgatggcg aagccctgtc
     2941 cgacatg
//
LOCUS       DBNGZC010000366         2649 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1301810, whole genome shotgun sequence.
ACCESSION   DBNGZC010000366 DBNGZC010000000
VERSION     DBNGZC010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1301810"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="AC16G0_06530"
     CDS             complement(<1..199)
                     /locus_tag="AC16G0_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099315.1"
                     /translation="MLILQQTRAAGAEMAFIRWTYVVFLALGGLAVVAGSVLFVRRMA
                     VPLRRLSAAALDVAGGNLSRQV"
     gene            203..844
                     /locus_tag="AC16G0_06535"
     CDS             203..844
                     /locus_tag="AC16G0_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099316.1"
                     /translation="MDVFQPLQSLFEKTGVGIQLGHRLMQRRWPAWLFLENGLDDAEE
                     LFQMPFHLLGERPLRHFFRVRRLILREVHSLKTVPQVSDVPERLDAVGIGSLVFQEKS
                     VPNIRQEMKEGTLQAVKDLDVDVQFHALSMKGPDMASFLYRQAADDGHQHEEENGEDS
                     EAPADTTPEDRAAVFFGCPQDATPLPRLRTYAGARQNQARGSLAERGPGWMQL"
     gene            complement(1106..1336)
                     /locus_tag="AC16G0_06540"
     CDS             complement(1106..1336)
                     /locus_tag="AC16G0_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099317.1"
                     /translation="MASRLLHSPWSNSRPPRSSQQEAVARFCHACVFFHETRAKFFPG
                     EAPDLVPPSCFYRGTPRASGWCGGASFQEGIP"
     gene            complement(1563..1994)
                     /locus_tag="AC16G0_06545"
     CDS             complement(1563..1994)
                     /locus_tag="AC16G0_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099318.1"
                     /translation="MASMAERGFRRQLTGKLRQYEGHLTKLEGQLMILRGKAERATGE
                     AQVKLSALMSEVERGVDGVRAAGRTALEGLERAAESGQALMDQMKGRLAEVEAMAPTV
                     MAKGRAAVQRATIEAKALRHGVKVGIRVARRVSKRVKAGNA"
     gene            complement(2208..>2649)
                     /locus_tag="AC16G0_06550"
     CDS             complement(2208..>2649)
                     /locus_tag="AC16G0_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099319.1"
                     /translation="RPLGNAEHSGTYNGHLISILAAEVCLREIRRPGFYDHLYALADR
                     LYSGMNAIFERRRFPARLQGLGARFGLYFGFTEEVRTYRDTARQDAELGLKFFETMAA
                     RGVYFCDAGGKPMHHGFTAAHTLADMDRVLQAAEDSVCSLGVTR"
BASE COUNT          473 a          894 c          829 g          453 t
ORIGIN      
        1 gcacctgacg cgagaggttt ccgcccgcaa cgtccagcgc cgcggcactc agccgtcgca
       61 ggggcacggc catccgtcgc acgaacagga cgctcccggc aacgacggcc agccccccaa
      121 gggccaggaa tacaacgtag gtccaccgga tgaaggccat ctctgcgccc gccgcccgcg
      181 tctgttgaag gatgagcatt cggtggacgt cttccaaccg ctgcaatccc tcttcgagaa
      241 gacgggcgtc ggaatccagc ttggtcaccg cctgatgcag cgccgttggc ccgcgtggct
      301 tttcctcgaa aatggacttg acgacgccga ggaactgttc cagatgccct ttcacctcct
      361 gggagagcgc cctctccgcc acttcttccg cgtccgacga cttattctcc gcgaggtgca
      421 ttcgctcaaa acggtcccac aggtctcgga tgtccccgag aggcttgacg cggttgggat
      481 cggttctctt gtcttccagg aaaagagcgt cccgaacatc agacaggaga tgaaagaggg
      541 cactctccaa gcggtcaagg acctcgatgt ggacgtccaa ttccatgctc tcagcatgaa
      601 gggacccgat atggcgagct ttttgtaccg acaggccgcc gacgatggcc accagcacga
      661 ggaagagaac ggagaggaca gtgaggcgcc ggctgatact accccggaag accgggccgc
      721 cgttttcttt gggtgcccgc aagatgcaac tcctctcccg cgactccgca catacgccgg
      781 agccagacag aatcaggcga ggggatctct tgctgaaaga gggccaggct ggatgcagct
      841 ttgaagaccg cggccagatg gccagcggcg aggcggctac tccgcgggtc cccctccctg
      901 gagtaacgtg ccccagccgg ctgaagggca atccttcctt cagtcgacgc aggaagtata
      961 catgctgagg ctaccttagg ctagccggta ttctcttact ggcgagcgat cgcaagtgtc
     1021 gggaccgtgg gtcatccgct catcgtgctg ttcgcacgtc ggctcccctg cggtcgccct
     1081 gacgtcacac acaggcggga gcgcctcagg ggatgccctc ttggaatgac gcgccgccgc
     1141 accagccgga cgccctgggg gtgccccggt agaaacaact gggcgggacc aggtcgggcg
     1201 cctctccggg aaagaacttg gcgcgcgtct cgtggaaaaa gacgcaggcg tggcagaagc
     1261 gggccaccgc ctcttgctgg ctgcttcgag gcggccggct gttcgaccac gggctgtgca
     1321 gcaacctgct ggccatcgcc gactccttac tgtgcggtgc gcaagatagg cgacagcccc
     1381 ccaggaccgc acagtccggg ggagagcgcg aggaggggtg gcggcccacc catctcggaa
     1441 gggtccgtgg ggagggctga gcccttgcgg tgggctctct gcaagggcga gggccttccc
     1501 cgcgaacgga gcagcgcggg gaacgatgta gccgttattc cgcgctgcgt cgtatgcgtg
     1561 ggtcaggcgt tacccgcctt gaccctcttg gaaacacggc gggcgacccg gatgcccacc
     1621 ttcacgccat gccgcagggc cttggcttcg atggtcgcac gctggacagc cgcccgaccc
     1681 tttgccatga cagtgggagc catcgcctcg acctccgcca ggcgaccctt catctgatcc
     1741 atcagagctt gtcccgactc cgccgcccgc tccagcccct ccagggcggt ccggccggcg
     1801 gcccgcacgc cgtcgacccc acgctccacc tcggacatga gtgcgctcag cttgacctgg
     1861 gcctcacccg tggcgcgctc ggccttcccc cgaaggatca tgagctgacc ctccagcttc
     1921 gtcaggtgcc cctcatactg gcgcagcttg cccgtcagtt gtcgccggaa tccacgctct
     1981 gccatgcttg ccatgttcgc ctccgatctg ctcgcgcccc ggcccacttc agaccacgcc
     2041 ggagcggcgg agatgggaaa ccgtaccgcc gaccattctc aaagagttct cagtcgtgaa
     2101 cccgctccgc cacagcctat cggccgtcgg gctcagacca ttgagatcag gataggagca
     2161 ggcatggcgg ttgtcaagac accgcgggtt gttccgggac cggggggcta gcgcgtgact
     2221 cccaaggaac acacgctatc ctcggcggcc tggagaacgc ggtccatgtc tgccagggta
     2281 tgcgcggcgg tgaacccgtg gtgcatgggt tttccgccag catcgcagaa gtacaccccg
     2341 cgcgccgcca tagtctcgaa aaacttgagg cccagttccg cgtcctgccg tgcggtgtcc
     2401 cggtaggttc tcacctcctc cgtgaaaccg aagtagaggc cgaaccgggc gcccagccct
     2461 tggagacgcg cggggaaccg ccgtcgctcg aaaatggcat tcattcccga gtagagccga
     2521 tccgccaagg cgtacaggtg atcgtagaac cccggccgtc ggatctctcg caggcagacc
     2581 tccgcggcca ggatggagat gaggtgcccg ttgtaggtcc cgctgtgctc cgcatttccc
     2641 aggggtcgc
//
LOCUS       DBNGZC010000367         1635 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_421487, whole genome shotgun sequence.
ACCESSION   DBNGZC010000367 DBNGZC010000000
VERSION     DBNGZC010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_421487"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            537..1541
                     /locus_tag="AC16G0_06555"
     CDS             537..1541
                     /locus_tag="AC16G0_06555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5099320.1"
                     /translation="MTIAPRVLVVDDEAPVRRQLKVGLVQHGYEVEESEEGLDALAKI
                     RSARTRTPFDFVVLDICLPDIDGLKLLNAIRGTYPQLPVVVISGYGTESTADSVESHN
                     GSAYLDKPFEVSTLVAEFTRIAHPEGVEPARIPENPPAVRPLESAFVFAKARPGADVF
                     GFFMRLTGAEGICYCDPLVGDWDLAVLLQAPDRRGIERLIATHLESSAEMGAYEVHAC
                     EKPRLPRECAAFLEEYDQMQAAQRRLAGEADRRKTGRLTAYAVLDVEPARLALLYMKL
                     RFTDHVIDCDVTDGGRKVLLLLQAGLDQELQETIRNEIRIMPGVLRIKLLNVLNFWSK
                     "
BASE COUNT          294 a          543 c          531 g          267 t
ORIGIN      
        1 tgcatcctcc cgccgcgggg atgaccatcg gcatgaaagc ccggattatc cacggatttc
       61 tcgtgaataa tccgggctaa gtgggccagt gcccgatcct cacctctctc aggcgaaacg
      121 cagatccctc ggtctcgccg tcctcgaccc ctctgcagga aatcgggagt gaccctccct
      181 cgtttcttgc gaagggacaa acagccccgc acacagcaag gagcgagaca caaaggccag
      241 cgagccggga tcatcccagg aggggctcgc ttggcatccg cgagaatgtg gaggcctttc
      301 gtagctttcc gttctttccg ggtgccctgc ccggggagcg ccacgggccc cgacgccgcg
      361 gggatcaaga gttttccatc ggttaggagc catggatgta ccagccccct ccgggaggcc
      421 actcggaggc atttcatggt ctgtgggagc ctgtgttggc acggaagtcg cttgtgaact
      481 ctggtgcaat aatccgcttg ttcccacgcg aggcactcgg ataaggagac cagcccatga
      541 ccatcgctcc gagggtgttg gtagttgacg atgaggcgcc ggtccggcgc caactcaagg
      601 tcggcctggt ccagcacggc tacgaggtgg aggagagcga ggaagggttg gacgccctgg
      661 caaagatccg gagcgcccgc accaggacgc cgttcgactt cgtcgtcctc gacatctgcc
      721 tccccgacat tgatggactg aagctcctca acgccatcag gggcacctat ccgcagctcc
      781 ctgtagtggt gatctccggc tacgggaccg agagcacggc ggacagcgtc gagagccaca
      841 acgggagtgc ctacctcgac aagcccttcg aggtgagcac cctggtcgcc gagttcaccc
      901 ggatcgcaca cccggagggt gtggagcccg cgcggatccc ggaaaacccg cccgcggtcc
      961 ggccgctgga aagtgccttt gtctttgcca aggcccgacc cggtgccgac gtgttcgggt
     1021 tcttcatgcg cctcaccggg gccgagggga tctgctactg cgacccgctg gtgggcgact
     1081 gggacctggc ggtgctgctc caagcgccgg accgccgggg catcgagcgg ctgatcgcca
     1141 cgcatctcga gtcgagcgcc gagatgggcg cctacgaggt ccacgcctgc gagaaacccc
     1201 ggctgcccag ggagtgcgcg gccttcctcg aggagtacga tcagatgcag gcggcccagc
     1261 gccgcctggc gggcgaggcc gaccggcgga agaccggcag gctcaccgcc tacgcggtgc
     1321 tcgacgtcga gccggcacgg ctggcgcttc tctacatgaa gctccgcttc accgaccacg
     1381 tgattgactg cgatgtgacc gacgggggcc ggaaggtcct gctcctgctc caggccgggc
     1441 tcgatcagga gctgcaggaa acgatccgca acgagatccg gatcatgccc ggcgtgctcc
     1501 ggatcaagct cctcaacgtg ctcaacttct ggagcaagta aggctcaccg ggaagaagga
     1561 cctgaccatg gcccaaccgc cgcccgccgc cgccccggcg gcgcacgcct ttggccggat
     1621 gctgtggcga caccg
//
LOCUS       DBNGZC010000368         1785 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1890298, whole genome shotgun sequence.
ACCESSION   DBNGZC010000368 DBNGZC010000000
VERSION     DBNGZC010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1890298"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1698)
                     /locus_tag="AC16G0_06560"
     CDS             complement(<1..1698)
                     /locus_tag="AC16G0_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HIR5099321.1"
                     /translation="MKGIVQMPAGPQVAVSTLPADLSVQYLAGGSARELPVILRAQIR
                     PTVVSPPEEFENFIFANGSIKEGITRRGVEPEESDEEEGPALFSPQGKPAIHQRDNLV
                     LDAAGTARATISRLPRVTSPQEVLAELEFRDPNGEVQTVSSRAPLWPASQLVGIQPDS
                     WVASRESLKAWLAVIDISRRPVQASSVRVELLRRSTYTHRKRLVGGFYAYEHVEETRR
                     IGELCRGLTDLKGRFLCEGKPPAEGNLILQATVTDPAGNTAAAHADVWVAGSERFWFD
                     IQDSDRMDLLPEKRRYEPGEKARLQVRMPFPEATALVTIEREGILEASVVPLSGKEPV
                     VEIPLKEQHAPNVFISVLAVRGRVSGVQPTALVDLGRPSFKLGVAEIRVGWRASELQV
                     KVSPERPTYRVREKAKVKVAVRRADGQPPPPGSEVALAAVDEGLLELLPNRSWNLLEA
                     MMRRRGYGVQTASAQMQVVGKRHYGLKALPQGGGGGRQTARELFDTLLLWKGRVTLDA
                     QGEASIEVPLNDSITSFRIVAVATGGIGLFGTGEATIRSTQDLMVLSGISPLVREGDR
                     "
BASE COUNT          285 a          638 c          569 g          293 t
ORIGIN      
        1 tcgatcgccc tcgcggacga gcggggatat gcccgacagc accataagat cctgcgtgga
       61 tcggatcgtg gcctctccag ttccaaacag tccgatccca ccggtggcca cggcgacgat
      121 ccgaaagctc gtgatcgaat cgttgagcgg gacttctata gatgcctcgc cctgggcatc
      181 cagggtcacg cgacccttcc agaggagcag ggtgtcgaac agctcccggg cggtctgtcg
      241 cccgccgcca ccgccctggg ggagcgcctt gaggccgtag tgccgcttgc ccaccacctg
      301 catctgtgcg ctggcggtct gcaccccata gccccggcgc cgcatcatgg cctcgagcag
      361 attccagctc cgattgggca ggagctccag gaggccttcg tccaccgcgg ccagggcgac
      421 ctcgctgccg ggcggaggcg gttggccgtc cgccctccgt accgcaacct tcaccttggc
      481 cttctcccgc acccggtagg tcgggcgttc cggcgagacc ttgacctgca attcggacgc
      541 ccgccagccg acgcggatct ccgccacccc cagcttgaag gatggccttc ccagatcgac
      601 caacgccgtg ggctgcacgc cgctcacccg gccacgcacc gccaagacgg agatgaacac
      661 attgggcgcg tgctgttcct tcagggggat ctcgacgacc ggctcctttc cggagagggg
      721 aacgacggag gcctccagga ttccttcccg ttcgatggtg acgagggccg tggcctcggg
      781 gaagggcatg cgcacctgca ggcgggcctt ctcgcccggt tcgtagcgcc gcttctccgg
      841 caaaagatcc atgcgatcgc tgtcctggat gtcgaaccag aagcgctccg atcccgccac
      901 ccacacgtcg gcatgcgccg ccgccgtatt gccggccgga tccgtgacgg tcgcctggag
      961 gatgagattg ccctccgccg gcggcttgcc ctcacagagg aatcgaccct tgaggtcggt
     1021 cagcccgcgg cacagctctc cgatccgccg cgtctcctcc acgtgctcat aggcgtagaa
     1081 gccgccgacg aggcgcttgc ggtgggtgta ggtgctccgt cgcagaagct ccacccggac
     1141 cgaggacgcc tgcacaggcc gtcgggaaat atcaatgacc gcgagccagg ccttcaagct
     1201 ctcccgagat gccacccagg agtcgggctg gattcccacc agttggctgg ccggccagag
     1261 cggggccctc gaggacacgg tctggacctc gccgttggga tcccggaatt ccagctcggc
     1321 caggacctcc tgcgggctgg tcacgcgcgg cagccggctg atggtcgccc gagccgtccc
     1381 ggcggcgtcc aggaccaggt tatcgcgctg gtggatcgcg ggcttcccct gcggcgagaa
     1441 gagggcaggc ccctcttcct cgtcactctc ctcgggctcc actccccgac gagtgatccc
     1501 ctccttgatc gagccgttgg cgaagatgaa gttttcgaac tcctcgggag gagagacaac
     1561 cgttggtctg atctgggcgc ggaggatgac cggcagctct cgtgcgctcc cgcccgccag
     1621 atattgcacg ctgagatccg ccggaagcgt cgagaccgcc acctgggggc cggcgggcat
     1681 ctggacgatc cccttcatca gggggacccg gaactcctcg acccggaacc cgccggatcg
     1741 ccaaccgaaa cgggagacct cctcatcctc ggcctccggc gcggg
//
LOCUS       DBNGZC010000369         3671 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1008385, whole genome shotgun sequence.
ACCESSION   DBNGZC010000369 DBNGZC010000000
VERSION     DBNGZC010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1008385"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..1177
                     /locus_tag="AC16G0_06565"
     CDS             281..1177
                     /locus_tag="AC16G0_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR5099322.1"
                     /translation="MRDCARVVGLLLLLGFPSARSASAADALILGRNIPILQAPPKTA
                     AVIKTGQPGETYEVVGRKTGKGQPLYTLDELGDLWMKVRLSDEASGYIRTDGVSVARE
                     EFRSPKGNPVLLVNLRPTADGSVVQELWVVSEGWHRTRRLALIEGRPVWAVSGDWFLC
                     QVDSERPIKSQTMDRNIERIERFSADGRSHSILAAGSYPLLNEARGEVYFYRDVDEQG
                     EAVRPGLFAVNVDGGNLHPLFFLPEGYRFWREDGDFFVQAPSPILNAAADSIRLYGFD
                     RRGVKNRFTITPEGHLFELRME"
     gene            1427..1912
                     /locus_tag="AC16G0_06570"
     CDS             1427..1912
                     /locus_tag="AC16G0_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5099323.1"
                     /translation="MTDPTWLEEAEVYFRKGYEAQMQGNLDEAIAHYLRSIQLCPTAE
                     AHTFLGWAYSSQGKPEEAIRECQIAIRIDPDFGNPYNDIGAYLIELGRLEEAIPWLKQ
                     AMLAKRYEPRHYPHMNLGRVYAKQGKLEDAIRELKRALEIEPDYLAARRELHRVLGML
                     N"
     gene            1959..3233
                     /locus_tag="AC16G0_06575"
     CDS             1959..3233
                     /locus_tag="AC16G0_06575"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR5099324.1"
                     /translation="MNARWIPGVLSLLVSLTAFLSSPTFSGSAVWAAAAAGNVPPEIA
                     TYFTPEDVARGHAYTEGRYWIAAAAIALRLLILVLLVLTPASANLRNLAVRLVPSRPT
                     VAVAVYLALLVVIYEVLTLPLGYYAGFIREHAFGLSTQTAAAWFLDRVKGALLTLAVV
                     VPLGSLLVLLWRRQPGRWVLPAWGLCSLVTVLLVALAPVVIDPLFNTVRPVLDPGLRQ
                     RVLALAEKAGIPVEQVYEIDASRRTRKGNAYFTGLGKTKRVVLYDTLISGSSPDEVEL
                     VVAHEMGHWKHADIWKGIGLSLIGLGISLWCGGRVLEWAARLGRFYLAGPADVAGVPL
                     FLLVLFVLNMASLPLQNAISRSFERAADRTSLELTGNTQAFIRSEVQLARSNLADLTP
                     PRALVWLLYTHPPVAERIRMAEQFAKKIQAHP"
BASE COUNT          563 a         1271 c         1165 g          672 t
ORIGIN      
        1 gaccagtcgt aagttttgcc accctggaga ttactcaatc caacatccca ccaatccgaa
       61 cctctcgatt gtcagacccc cggatttccg ggctgccgag ttacccgtct gcccgatttc
      121 ccgggcatgc ggcggttgac aatcgagagg ctttccgcca tgctattctg ctagctagag
      181 tcaccggttc cgttacccct tcgctccgca agctccgcag gtgtgcatga cacagttgac
      241 ccgaaaccca tggcagcagc caagaacccg gatgggacgc atgcgtgact gtgcccgggt
      301 ggtcggcctg cttctcctgc tgggatttcc ctccgctcgt tcggcctcag ccgcggacgc
      361 cttgatcctg ggccgcaaca tcccgatcct ccaggcccct cccaagacgg cggcagtgat
      421 caagaccgga cagcccggag aaacctatga ggttgttggc cgcaagaccg gcaaggggca
      481 accgctctac accctggatg agctggggga cctctggatg aaggtccggt tgagcgacga
      541 ggcgtcgggt tacatccgaa ccgatggcgt gtccgtcgcc cgcgaggagt tccgctcccc
      601 caaaggaaat ccggtactgc tcgtcaacct ccgccccacc gccgacgggt cggtcgtgca
      661 ggagctttgg gtggtctcag agggctggca tcgcacccgg agactggccc tcattgaggg
      721 ccgacccgtc tgggccgtta gcggggactg gttcctctgc caggtggact ccgagcggcc
      781 catcaagagc cagacgatgg atcggaacat cgagcgcatc gagaggtttt ccgccgacgg
      841 tcggagccac agcatcctgg cggctgggtc ctaccccctc ctgaacgagg cgcggggcga
      901 ggtgtatttt taccgcgacg tggacgagca aggggaggcg gtccgacccg gcctgtttgc
      961 cgtgaacgtg gacggcggga atctccatcc cctgttcttc ctcccggagg ggtatcgatt
     1021 ctggagggag gatggcgatt tcttcgtcca ggcgccctca cccatcctga atgccgccgc
     1081 tgactccatc cgcctgtacg gcttcgaccg tcggggggtg aaaaaccgtt tcaccatcac
     1141 cccggagggc catctcttcg agcttcgcat ggagtgagct gacggccccg cgttgtcctc
     1201 catatccccc tccgcttccg tcccggactc ccgcggcccg gagatttccc gaggcaattc
     1261 gtggagtatc cgggcgagac gcatccggtc ccgtgcttgc cacagccggg gtgcctgggt
     1321 atactgctct cgtccgctgc aacgcctggg gcattcgcca ccggagcgct ccgggagcga
     1381 cgagcgggtt cccggcagac gtcgcacggc ggggagggat gctgtcatga ccgatccgac
     1441 ctggctggaa gaggcggagg tttatttccg caagggatac gaggcccaga tgcagggcaa
     1501 cctggacgag gccatcgccc actacttgcg ctccatccag ttgtgcccga cggccgaggc
     1561 gcacaccttc ctgggctggg cctacagttc ccagggcaag ccagaagagg ccattcgaga
     1621 gtgccagatt gccatccgga tcgacccgga tttcgggaac ccgtacaacg acatcggcgc
     1681 ctacctgatt gaactcggcc ggctggaaga ggccattccg tggctcaagc aagccatgct
     1741 ggccaagcgg tacgagccgc gtcactaccc gcacatgaac ctgggacggg tctacgccaa
     1801 gcaggggaag ctggaggacg cgatccggga gctgaagcgg gccctggaga tagagccgga
     1861 ttacctggcc gcacgccgcg agctgcaccg cgtcctgggg atgttgaact gatggccgca
     1921 ccggctcggg tgggtcgtgc gccgggggac cccccccgat gaacgcccgg tggattcccg
     1981 gtgtgttgtc gctcctcgtc tccctgacgg ccttcctgtc ttcccctacc ttctcaggct
     2041 ccgccgtgtg ggcggccgcc gcggcaggga acgtcccgcc cgagatcgcc acctacttca
     2101 cgcccgagga cgtggctcgc gggcacgctt acaccgaggg gcgctactgg atcgcagccg
     2161 ccgccatcgc cctccgcctc ctgatcctgg tgctcctggt tctgacccca gcctcggcca
     2221 atcttcgcaa cctggccgtg cgcctcgtgc cctcccggcc caccgtcgcg gtggctgtct
     2281 atctcgcgct cctggtggtg atctacgagg tcctcactct ccccctgggc tattacgcag
     2341 gcttcatccg ggagcacgcc tttgggctct ccacccagac ggcagccgcc tggttcctgg
     2401 atcgcgtgaa gggcgccctc ctcaccctgg ctgtggtcgt ccccctgggg tcgctcctgg
     2461 tcctcctctg gcgccgccaa ccggggcgct gggtcctgcc cgcctggggc ctgtgcagcc
     2521 tggtcaccgt cctcctcgtg gcgctggcgc ccgtcgtcat cgacccactg ttcaacaccg
     2581 tccgtcccgt gctggatccc gggctccgcc agcgggtcct ggccctggcc gagaaggcag
     2641 gcatcccggt cgaacaggtc tacgagatcg acgccagccg ccgcacccgg aaggggaacg
     2701 cctacttcac cgggctcggg aagacgaaac gcgtcgtcct gtatgatacg ctcatcagcg
     2761 ggagcagccc cgacgaggtg gagctggtgg tggcgcacga gatgggccac tggaagcacg
     2821 ccgacatctg gaagggtatc ggcctgagcc tcatcggcct gggcatcagc ctctggtgcg
     2881 gcggccgggt cctggagtgg gccgcccggc tcggtcgctt ttatctggca ggcccggcgg
     2941 acgtggcggg tgtgcccctg ttccttctcg tcctcttcgt cctcaacatg gcgagcctcc
     3001 ccctccagaa cgccatctcc cggagcttcg agcgcgccgc cgaccggacc tccctggagt
     3061 tgacggggaa cacccaggcc ttcatccgct ccgaggtaca gctcgcgcga tccaacttag
     3121 ccgacctcac cccgccccgc gccctcgtct ggctgctcta cacccatccc cccgtggcgg
     3181 agcgcatccg catggccgag cagttcgcca agaagatcca ggcccacccc tgatggggag
     3241 atgcttctgg gtctcggggg ccggccagcc ggcgacgtcg gccccccatg cgcgcgggtg
     3301 tccatccacc tgaaactggt ttgatcgaag gcgggactcg gaggaccttc aggcgtgcgg
     3361 cggcgtggag cgccacccgt tctgcggcgc cggcgcagcg tccacaaatt atcggagtcg
     3421 gtagcggatt attctttcac cgttggcggc cgcttcaaga gccagtcgat gacatcccct
     3481 ggatcggggg aatcgcccct tcgactggga acctcgtcct gggcggctgc tggcggtgga
     3541 ggcgcctggc gaggggctgc tctctctttc cttggcgctg aagggctctg cgtcactcgc
     3601 ggcttcttcc ctccccgagg tgcgactgcc cgtgtcggag atccttgcgg cgccaggtcc
     3661 tcggagggag g
//
LOCUS       DBNGZC010000370         1859 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1104583, whole genome shotgun sequence.
ACCESSION   DBNGZC010000370 DBNGZC010000000
VERSION     DBNGZC010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1104583"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="AC16G0_06580"
     CDS             complement(<1..299)
                     /locus_tag="AC16G0_06580"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF012436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HIR5099325.1"
                     /translation="MLSKAVQNAINEQINAELYSAYLYLSMSAYCAATNRPGSAGWMR
                     IQAQEELGHAMKFFGYVIDRGGRALLKAVGQPPAEFKSLLDIFQQALEHEEEVTR"
     gene            complement(358..774)
                     /locus_tag="AC16G0_06585"
     CDS             complement(358..774)
                     /locus_tag="AC16G0_06585"
                     /inference="COORDINATES: protein motif:HMM:NF021252.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HIR5099326.1"
                     /translation="MLAALQAKLGIGDALVFKAGSALSGGMVGQGETCGALTASIMAI
                     GCQVGRERLEDLAQFQRAKVPATEMYHRFREQIGHSLCAEIHKIRYGRTYRLYDPAEV
                     EAFHEAGGHDRTGCPQICGIAARTAADIILRLLEPA"
     gene            complement(919..1359)
                     /locus_tag="AC16G0_06590"
     CDS             complement(919..1359)
                     /locus_tag="AC16G0_06590"
                     /inference="COORDINATES: protein motif:HMM:NF014091.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X swivel domain-containing protein"
                     /protein_id="HIR5099327.1"
                     /translation="MSSEQVRFQGHGCAAGVFEGEVVVSSQPFGFWQGMDPQTGVVID
                     KRHDACGLSVKGKAFVFPFGRGSTGTPGIFLEAVRNGCAPRAIVNVKSEPMIIMCALL
                     AEAFFKISIPIVDGLDVNPMEVLRTGDRIRINGTTGVIEVVARG"
     gene            complement(1356..>1859)
                     /locus_tag="AC16G0_06595"
     CDS             complement(1356..>1859)
                     /locus_tag="AC16G0_06595"
                     /inference="COORDINATES: protein motif:HMM:NF016310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HIR5099328.1"
                     /translation="LYHMVGITPEARTLEEACGGRVPAERIAVGRRDLEAVMDQMGAK
                     DGPVDVVGIGCPHASIDQMRRYASLLEGKRVHSNTQLWICTNVVVEEMARKMGYVETI
                     ERAGARLMVGTCHNDCPLEAWGFRRLATDSGKFAYYTPTTVGTECVFASTEACIQAAI
                     TGKVERR"
BASE COUNT          296 a          659 c          568 g          336 t
ORIGIN      
        1 ttgcgcgtca cctcttcctc gtgctccaga gcctgctgga agatgtccag cagggacttg
       61 aactccgcgg gcggctgacc gacggccttg agcagcgccc gtccgccgcg atcgatcacg
      121 tagccgaaga acttcatggc gtgcccgagt tcctcctggg cctggatgcg catccatccc
      181 gcggagcccg gccgattcgt ggccgcgcag taggctgaca ttgacaggta caggtaggcg
      241 gagtagagct cggcgttgat ctgctcgttg atcgcgtttt gcacggcctt gctcagcatg
      301 gtcgccctcc tctcagtggt gtgtggtcct gcgtctcacc tcgatcggga ttcctgctca
      361 tgcaggctcg agcagacgca ggatgatgtc ggcggcggtc cgggcggcga tcccgcagat
      421 ctgcggacag ccggttcgat cgtggccccc tgcctcgtga aaggcctcca cctccgcggg
      481 atcgtacaga cgataggtcc gcccgtatcg gatcttgtgg atctcggcgc acaagctgtg
      541 cccgatctgc tcacggaagc ggtgatacat ctccgtcgcc ggaaccttgg cgcgctggaa
      601 ctgggccaga tcctccaggc gctctcgccc cacctggcag ccgatggcca tgatggacgc
      661 agtcagcgcg ccgcaggtct ccccctgtcc caccatgcct ccggaaaggg cgctgcccgc
      721 cttgaacacc agggcatcgc cgatgcccag cttcgcctgc agagctgcaa gcacggcctg
      781 ggttcagccg aagtagttca tctcgttctg cagagccagc tcgtagacct cgttgagcag
      841 cgcgtcgcgt gtggccatgg cttcctcccg cgatgacatt ccaaattcga gccgcgagcc
      901 gagagccgcg tgcggcgatc acccccgagc gaccacctcg atcaccccgg tggtgccgtt
      961 gatccggatc cgatcccctg tgcggaggac ctccatgggg ttcacgtcca gcccgtccac
     1021 gatggggatc gagatcttga agaaggcctc ggccaggagg gcgcacatga tgatcatggg
     1081 ctcgctcttc acgttgacga tcgcccgagg ggcgcagccg ttccgcacgg cctccaggaa
     1141 gatgcccggc gtgccagtgg acccgcggcc gaaggggaac acgaaggctt tgcccttgac
     1201 cgacaggccg caggcgtcgt ggcgcttgtc gatcacgacc cccgtctgcg ggtccatccc
     1261 ctgccagaat ccgaagggct ggctcgacac cacgacctcc ccctcgaaca ccccggccgc
     1321 acagccgtgc ccctggaacc ggacctgctc cgagctcatc gtcgctccac tttccctgtg
     1381 atggccgcct ggatgcatgc ctcggtgctg gcgaatacgc actcggtccc gacggtggtc
     1441 ggggtgtaat aggcaaactt ccccgagtcg gtcgccagcc ggcggaaccc ccaggcctcc
     1501 agggggcaat cgttgtggca ggtccccacc atcaatctgg ccccggcccg ctcgatggtc
     1561 tccacgtatc ccatcttccg ggccatctcc tccaccacga cattggtgca gatccagagc
     1621 tgcgtgttgg aatggacccg cttgccttcc aggagcgagg cgtagcgccg catctggtcg
     1681 atggaggcat gcgggcaccc gatacccacc acgtccaccg gcccatcctt ggcccccatc
     1741 tggtccatga ccgcttcgag gtcccgccgt cccaccgcga tccgctcggc agggacgcgg
     1801 cccccgcagg cctcctccag cgtccgggcc tcgggcgtga tgcctaccat gtgatacag
//
LOCUS       DBNGZC010000371         4135 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1890443, whole genome shotgun sequence.
ACCESSION   DBNGZC010000371 DBNGZC010000000
VERSION     DBNGZC010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1890443"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..671
                     /locus_tag="AC16G0_06600"
     CDS             <1..671
                     /locus_tag="AC16G0_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099329.1"
                     /translation="RDLSTLGAAAAAWNLTGSAAWGQLRKFPGTTLNVLSSSGHRQLD
                     PIWNKLGELEQETGIKVNLTRVPTGEIRQKIMQDLVMGTGQFDVYEPPDDTIYSASQY
                     LAPLEPYIQKDFGSVEAWGKYTVPWALKAASVRGDVRCHPYYSGTVAAAYRENLFTNP
                     KNRADFKGRFGYDLPTPPKNWKELVDAAQFFTREEGGQQQWGLVFPGKQDPGLNIFEM
                     LVFE"
     gene            675..1352
                     /locus_tag="AC16G0_06605"
     CDS             675..1352
                     /locus_tag="AC16G0_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 periplasmic-binding domain-containing
                     protein"
                     /protein_id="HIR5099330.1"
                     /translation="MEYLDDQNHSLWGPKHPEHQATVAKVAAFMQDLIYKQKVSPSTI
                     PGMATNESVDLYLNGKSAMILDLIYFAWNEFVSKKVTDRIGRSISFQVPSSSGAGQGG
                     IPFYWMMGINGKTGKKDAAWEFLRWFMREDNLKLALTKGTGVFVPTDTRLGDWAAAEN
                     VLPPAIVAASKRAQVYRLNPQIGQVRNTVRIYVEKLDLKDLTPEQFVQQSGNAVEQLM
                     ITTGLAK"
     gene            1372..2325
                     /locus_tag="AC16G0_06610"
     CDS             1372..2325
                     /locus_tag="AC16G0_06610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5099331.1"
                     /translation="MQPRERAVRAGSPRAVQHGSHRNIQRSWDTELWWFLLPAAVAVV
                     GVIVAPLAQVLYLSLFDYNIMQYTYQFVGLGNYGKILHDAEFGRALLRTWWYVAAVIG
                     TDFVYGFLVALLAWQLPLFWSKLFRAIVILPMLLIPSAAAMLWRSVIYAPPYAAFDQV
                     FGLPASVNILASPFTALWGIILTAIWGWSPWVFLLLLAGLEGLPVDPLEAAKLDGATF
                     PQLVWHIILPMMKPVIFITLSLKAVDSFLSFTFPWIMTEGGPGGSSHVLSTYIFEKAF
                     KFLDYGYGSSLGVVMLAIAAFTSTAVIIYAWKQGYVGNEAA"
     gene            2312..3133
                     /locus_tag="AC16G0_06615"
     CDS             2312..3133
                     /locus_tag="AC16G0_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5099332.1"
                     /translation="MKRPSIGGLARALGIVFVLLWVGIPFFSLVSVSLAPEGAAAVAL
                     GLPQPISLEHYRRVLTGLESLWLYLKNSVIVSFLATTMALGLGLPCAYGLSRLQRYAA
                     ARALYLAVFVLRMLPPITLVIPYFILVGKLGLLDTKVGLLYALLAPTLPLAIWTAKTF
                     FDAVPSTLDEAARIDGATGWQIFTRVALPLIGNGIAVTAILTFLTAYIDYIFAVTLTN
                     HEALTLPAYIAGFKTEYRFYVGDMLAATLVGTLPMVGLFLIVQRHMRRVVLAGVH"
     gene            3136..4074
                     /locus_tag="AC16G0_06620"
     CDS             3136..4074
                     /locus_tag="AC16G0_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013323.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR5099333.1"
                     /translation="MADKVLTLSQAVAGIPDGANLTFGGFAAYFTPLAFVRELIRQGQ
                     RDLEMTSVAECWVADYLAGADRLRCARFSNFMFEGLGRCRNFVRGVEQGTIAVEDYSH
                     FAITSRLVAAGMGLPFMPVRSMAGTDLMERRVLEPDKARLLDCPFTGEPVVLVPAARP
                     DVAVLHVARCDRDGNSQLFGYTAVVEEQARAAGRVIVTCEEVVEDTVIRAQPELTVLP
                     GFLVDAVVELPFGAHPTGMYRYYDPDFAFMQAYYEASREPAAFRAHLEEWVLGPADHW
                     ADLEKVGLRRLLALRSDPALGIARQHARRAACAPKT"
BASE COUNT          673 a         1313 c         1381 g          768 t
ORIGIN      
        1 tgcgggatct ctcgacgctg ggcgccgctg cggcggcctg gaacctgacc ggctccgccg
       61 cctgggggca gctccgcaag ttccccggca ccacgctgaa cgtcctctcg tcctcggggc
      121 atcgccagct tgatccaatc tggaacaagc tcggcgagct ggagcaggag acggggatca
      181 aggtgaactt gacccgggtg ccgacggggg agatccggca gaagatcatg caggacctgg
      241 tgatggggac ggggcagttc gatgtgtacg agccgccgga tgacaccatc tacagcgcct
      301 cgcaatacct ggcccccttg gagccgtaca ttcagaagga cttcggttcc gtggaggcct
      361 ggggcaagta caccgtcccc tgggcgctca aggccgcgtc cgtccggggg gatgtgcgct
      421 gccatccgta ttacagcggc acggtggcgg ccgcctaccg ggagaacctc ttcaccaacc
      481 cgaagaaccg ggccgacttc aagggacgct tcgggtacga cctccccacg ccccccaaga
      541 attggaaaga gctggtggat gccgcccagt tcttcacccg ggaggaaggc ggtcaacagc
      601 aatgggggct ggtctttccc ggcaagcagg acccgggtct gaacatcttt gaaatgctgg
      661 tgttcgagta gggggtggag tacctggacg accagaacca ttccctgtgg gggccgaagc
      721 atccggagca tcaggccacc gtggcaaagg tagcggcgtt catgcaggac ctgatctaca
      781 agcagaaggt gtcccccagc acgattcccg ggatggccac caacgagtcg gtggacctgt
      841 acttgaacgg caagtcggcc atgatcctgg acctgatcta ctttgcctgg aacgagttcg
      901 tctccaagaa ggtgaccgac cggatcgggc ggtcgatcag cttccaggtt cccagctcca
      961 gcggcgccgg gcagggcggg attcccttct actggatgat ggggatcaac ggcaagaccg
     1021 ggaagaagga cgctgcctgg gagttcctgc gctggttcat gcgcgaggac aatctgaagc
     1081 tcgccctcac caaaggcacc ggcgtgtttg tgcccacgga cacgcgcctg ggggattggg
     1141 ccgcggcgga gaacgtactg cccccggcaa ttgtggcggc cagcaaacgg gcccaggtgt
     1201 accgcctgaa tccccagatc gggcaggtcc gcaacacggt ccggatctac gtggagaagc
     1261 tggacctgaa ggatctcacg ccggagcagt tcgtccagca gtcggggaat gcggtggagc
     1321 agctcatgat caccaccggc ctggccaaat agggcgggct gaggactcgg ggtgcagcct
     1381 cgtgaaaggg cggtgcgggc cggctcgccc cgcgccgttc aacacggttc ccaccggaac
     1441 atccaacggt cctgggacac cgagttgtgg tggttcctcc tgcccgcggc ggtggcggtc
     1501 gtcggggtga tcgtggcccc gctggctcaa gtgctctacc tcagcctgtt cgactacaac
     1561 atcatgcaat acacgtatca attcgtcggg ctgggcaact acggcaagat cctgcacgac
     1621 gccgagtttg gtcgagccct gctgcggacc tggtggtacg tggcggccgt catcggaacg
     1681 gatttcgtgt atggattcct ggtcgcgctg ctcgcgtggc agctcccgct cttctggtcc
     1741 aaactgttcc gggccatcgt gatcctgccg atgctcctca tcccctccgc ggccgccatg
     1801 ctctggcgca gcgtcattta cgccccaccc tacgccgcct tcgaccaggt gttcggtctc
     1861 ccggcatccg tgaacatcct ggcgtcgccg ttcaccgcgt tatggggcat catcctgacg
     1921 gccatttggg gctggtctcc ctgggtgttt ctcttgctcc tggcgggtct ggagggactg
     1981 cccgtcgatc ccctggaggc cgccaagctg gatggcgcga ccttccctca actggtatgg
     2041 cacatcatcc tcccgatgat gaagccggtg atattcatca cgctcagcct gaaggccgtg
     2101 gactccttcc tctcgttcac ctttccctgg atcatgaccg agggagggcc gggcggctcg
     2161 tcgcacgtcc tgagcaccta catcttcgag aaggcgttca agttcctgga ctacgggtat
     2221 ggctccagcc tgggagtcgt gatgctggcc atcgccgcct tcacctccac ggcggtcatc
     2281 atctacgcct ggaagcaagg gtacgtcggc aatgaagcgg cctagcatcg gcgggctggc
     2341 gagagcgctg ggcatcgtct tcgtgctgct ctgggtgggc attccgttct tcagcctggt
     2401 ctccgtctcc ctggcgcccg agggggcggc ggccgtggcg ctcggattgc cccagccgat
     2461 ctccctggag cattaccggc gcgtcctcac gggcctggag agtctgtggc tttacctcaa
     2521 gaacagcgtg atcgtgagct tcctggcgac gaccatggcc ctggggttgg ggttgccgtg
     2581 tgcatacggc ttgtcgcggc tgcagcggta tgccgccgcg cgtgccctgt atctggccgt
     2641 attcgtcctg cgcatgctgc cgccgatcac cctggtcatt ccgtacttca tcctggtggg
     2701 caagctaggc ctcctggaca cgaaggtcgg gctcctctac gccctcctgg cgcccacgct
     2761 cccgctggcc atctggacgg ccaagacgtt cttcgatgcg gtgccaagca cgctggacga
     2821 ggcggcccga atcgacgggg cgaccggctg gcagatcttc acgcgggtgg ccctgccgtt
     2881 gatcggcaat ggcatcgcgg tcaccgccat cttgacattt cttaccgcgt atatcgacta
     2941 catcttcgcg gtgacgctga ccaaccatga ggccttgacc ctgccggcct acatcgccgg
     3001 gttcaagacc gaataccgct tctacgtggg cgacatgctg gcagcgacct tggtcgggac
     3061 gctgcccatg gtcgggctgt tcctgatcgt gcagcggcac atgcggcggg tcgttctggc
     3121 gggggtccat taagcgtggc agacaaggtc ctgacgctct cccaggcggt ggccggcatc
     3181 cccgacgggg cgaatctcac cttcggcggg ttcgccgcat acttcacccc gctggcattc
     3241 gtccgggagc tgatccgcca aggccagcgg gacctggaga tgacctccgt ggcggaatgc
     3301 tgggtggccg attatctggc aggagccgac cgcctgcgct gcgcccggtt ctcgaatttc
     3361 atgttcgagg gcctgggacg gtgccggaac ttcgtgcgcg gcgtggaaca gggcacgatc
     3421 gccgtcgagg attacagtca cttcgccatc acctcgcggc tggtggcggc gggcatgggc
     3481 ctgccattca tgccggtaag atccatggcg gggaccgacc tgatggagcg ccgggtgctg
     3541 gaacccgaca aggcacggct gctggactgc ccgttcaccg gcgagccggt ggtgctggtc
     3601 ccggcggccc ggcctgacgt ggctgtgctg cacgtcgcgc gctgtgaccg agacggcaac
     3661 agccagctct tcggctacac ggcggtggtg gaagagcagg cccgggcggc ggggcgggtg
     3721 atcgtcacct gcgaggaagt cgtcgaagac acggtgatcc gcgcccagcc agagctgacc
     3781 gtgctgccgg gattcctggt ggacgcggtg gtggaactgc cgttcggcgc gcatcccacc
     3841 gggatgtacc ggtactatga tccggacttc gcgttcatgc aggcctacta cgaggcgtcg
     3901 cgcgaaccgg cggcgtttcg cgcccacctc gaggagtggg tgctgggtcc tgcagatcat
     3961 tgggccgacc tggaaaaggt cggattgcgc cggctgctgg cactgcgcag cgacccggcc
     4021 ctcggcatcg cgcgccagca cgcgcgaagg gcggcatgcg ccccgaagac gtgacgcggc
     4081 aagagttcat ggcagcggct gccgcgcgag aggtccggga cggcgaggtc gccgg
//
LOCUS       DBNGZC010000372         1218 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2182347, whole genome shotgun sequence.
ACCESSION   DBNGZC010000372 DBNGZC010000000
VERSION     DBNGZC010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2182347"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="AC16G0_06625"
     CDS             complement(<1..668)
                     /locus_tag="AC16G0_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR5099334.1"
                     /translation="MTDEAAMHPAKILIVDDDSEIVNVLTELLGKDGHQVTSCVTGAA
                     AVALGDAERFDVILVDIRLPDMDGLAVLRSFQGSSPDSAVILMTAFGTVEVAIQAIKA
                     GAYDYVPKPFKLDEVRIAVQRALERKRLLQENRQYRQELQGKYRLENVVGASGPMLDI
                     FKAVARVAPTKSTVLLQGESGTGKELIARTIHYNSDRAHGPFITVDCGALPETLLESE
                     LFGH"
     gene            complement(658..>1218)
                     /locus_tag="AC16G0_06630"
     CDS             complement(658..>1218)
                     /locus_tag="AC16G0_06630"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5099335.1"
                     /translation="VKERLRLIQGHVDRVVAAARRFLDAARPEPSRVPVDLNALLEDL
                     LVLTSPEAQRKGIGVSRLLDQGIAPVPADPAQMQELFLNLITNALETMEDGGLLTVIT
                     EVSCEDGGIPGIRVILNDTGPGMAPEVSERAFEPFFTTKTSQRGTGLGLAICRRIARD
                     HGGSIRLESSPGQGTRAVVELPARDG"
BASE COUNT          201 a          395 c          391 g          231 t
ORIGIN      
        1 tcgtggccga acaactcact ttccaggagg gtttcaggca aggccccgca gtccaccgtg
       61 atgaacggcc cgtgggcccg gtcgctgttg taatggatgg tgcgggcgat gagttccttc
      121 cctgttccac tctccccctg gaggagcacc gtactcttcg tgggggcgac ccgggccacg
      181 gccttgaaga tatccagcat ggggccgctg gcccccacca cgttctccag ccggtacttc
      241 ccctgcagtt cctgccgata ctgtcggttc tcctggagca ggcgctttcg ctccaatgct
      301 cgctgcaccg cgatgcgcac ctcgtccagc ttgaagggtt ttgggacgta gtcataggcg
      361 ccagccttga tggcttggat ggccacctcc acagtgccga aggcggtcat caggatcacc
      421 gccgagtccg ggctggaccc ctggaaggac cggagcacgg cgagcccgtc catgtcgggt
      481 aggcggatgt cgaccaggat cacatcgaaa cgttctgcgt ctccaagcgc gacggcggct
      541 gcccccgtca cgcaggaagt tacctggtgg ccgtccttcc ccaggagttc tgtgagaacg
      601 ttgacgatct cggagtcatc atccacgatg aggatcttcg ccgggtgcat ggctgcctca
      661 tccgtcacgg gcggggagtt ccacgacggc gcgcgtgccc tgaccggggc tgctctccag
      721 gcggatcgaa cccccgtgat ccctggcgat gcggcgacag atggccaggc ccagcccggt
      781 tccccgctgg gatgtcttcg tggtgaagaa cggctcaaag gcgcgctccg agacttctgg
      841 ggccatgccc ggcccggtgt cattcaggat gacacggatc cccgggatgc cgccatcctc
      901 gcaggagacc tcggtgatga cggtcaggag accgccgtcc tccatggttt ccaatgcgtt
      961 tgtgatgaga ttcaggaaga gctcttgcat ctgagccggg tctgcgggaa ccggcgcaat
     1021 tccttgatcc aggaggcgac tgaccccgat ccctttgcgc tgggcctcgg gcgaggtgag
     1081 caccaggaga tcttccagga gagcgttcag gtccaccggg acgcgggatg gctctggtcg
     1141 ggccgcatcc aggaagcgcc gggcggccgc caccacccgg tccacgtgac cctgaatcag
     1201 gcgcaagcgt tctttcac
//
LOCUS       DBNGZC010000373         4746 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_519083, whole genome shotgun sequence.
ACCESSION   DBNGZC010000373 DBNGZC010000000
VERSION     DBNGZC010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4746)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4746)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4746
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_519083"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="AC16G0_06635"
     CDS             complement(<1..776)
                     /locus_tag="AC16G0_06635"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR5099336.1"
                     /translation="MEAIEARGITTPFATEMSSWGRPAVALSDAADRLSEVAYVVTTA
                     GFAAVMALGVFFRYALNNSLAWADEMAMIFFVWATFLSIATGYLHGKQVGVHFIVQAL
                     STRWRSVAAFLADSLAGGFLVSLLVSGMEALPLAEGAHTDALRLPVTIAYLAIPVASV
                     LMLIHWVRLNLAGGKRASCLGKVMGVTAIFSIIYLPLGHYVSLIGAPRYWLLTLSFLV
                     PLFIGVPVAFALGLLAMTYISVFGTIPFSTGAMQIFFGIE"
     gene            complement(961..2013)
                     /locus_tag="AC16G0_06640"
     CDS             complement(961..2013)
                     /locus_tag="AC16G0_06640"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR5099337.1"
                     /translation="MGKMCFGKRPRISFAFVCALVVCAAPPALAAGPVILRAGASEPE
                     GTTAPAMAIGKLCELANKLSNGELNVQPFYQSLGVEQQLAAAVKGGSVDIGYISTSNV
                     ARFTDAFLQFDLPFLFKNDRAYIDMLKSHPAGKKALAQFEKDLGVKTLLVTSHSYDAE
                     VSGTDLQTRDTPGRVPADIKGLKLRSTSTPVEIAVVKAYGANPTPVDWGQLYTALQQG
                     VVDGNAGTPLPPYASIKLYEVTKYYAAIGFRNNLFPIYMNQKKFESLTPFQQKALLDA
                     AAQTEALAGQYGREKVKAAIGEVEKAGVRIYHPNKEEMAQWMSVRETVWQVVADQFKG
                     KIDLGIANEIFKLNPR"
     gene            complement(2041..3948)
                     /locus_tag="AC16G0_06645"
     CDS             complement(2041..3948)
                     /locus_tag="AC16G0_06645"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR5099338.1"
                     /translation="MGAKLEAGTTTPLAVTSSVWGRSVVALSDAVDRACTFGAVMATA
                     GFGAIMLVGVFFRYVLNNSLAWSDEVALMVFMWATLLFIATGYLHDRHVNLDILVQKL
                     PPAWEARATVLAEGLSLAYLVSLTVSGIQALPLASQMHTDALRWPLTIPAVAIPVSCL
                     IMDLHWVRRNLLTGIRTAAAVKLLIAGTFLFLVMLPIGQYVQLTGLPRAAVLIVALFG
                     PMLLGVPVSLSLGFMATFYIGAFGDAPFDVGAQQIYNGMSLLALMAIPLLILAGKLMH
                     EAGIAQYIVDFAQVLVGRFRGGLGAANVVASFLFGDISGSAVSDTAAIGSLMIPQMKA
                     RGYRADFCAALQGTAGTLGMMAPLAITILLYASATNASVSRLAAATILPAFLLAGSFM
                     LVTLVHARRYNYPREYVPRDSILPRILRALPGMFALVLVLGGILGGVFTPAEVGAVLL
                     AYVLVLSFFYKTAQPGRLYKAAVDAGYISGMTLFMASTSSFLGFVMARDLVSQHVVEF
                     VTQISTNRLVVILVVSLVFIILGMILEPPAMIFGFLPSFMPLLGKVGVDIVHWGVLFC
                     TNMGLGCIIPPVALNLFVSTQLADVRYEDAVRACIPFIVIMVIDLAIMAVFPMIPLML
                     PHLLFDYPLPK"
     gene            complement(4093..>4746)
                     /locus_tag="AC16G0_06650"
     CDS             complement(4093..>4746)
                     /locus_tag="AC16G0_06650"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR5099339.1"
                     /translation="GKNAVTQFEKDLGVKALLWTSHAYDADFSGTDLQTRNKPVKVPA
                     DIKGLKLRTGSTPVEQFLVKAYGANPTPVDYGQLYSAIQQGVVDGNAATPLPPYAAIK
                     LYEITKYYVAIGFRMNLLPIYMNRKKFDSLTPFQQKALLDAAAQTKPLAGQWARERVK
                     LAADVVEKNGVHIYHPIKEEMAQWMSVREKVWQQVADEFKGKVDLKVANDVFKSIPR"
BASE COUNT          898 a         1473 c         1572 g          803 t
ORIGIN      
        1 tgttcgatgc cgaagaagat ctgcatggcc ccggtcgaga aggggatggt gccgaagacc
       61 gaaatgtacg tcatggccag caggccgaga gcaaaagcca ccggtacgcc gatgaagagg
      121 ggcacgagga atgacagcgt caacagccag tagcgcgggg caccgatcag ggacacatag
      181 tgaccgagcg ggagatagat gatgctgaag atggccgtga cgcccatgac cttgccgagg
      241 caggaagccc gtttgccccc ggccaggttc agacgcaccc agtggatcag catcagcacg
      301 ctggcgacgg ggatggcgag gtaggctatc gtgacgggca gccgcagggc gtcggtgtgg
      361 gctccctcgg cgagagggag cgcctccatc cccgaaacga ggaggctcac caggaagccg
      421 ccggccaggc tgtcggcgag gaaggcagcg acgctccgcc atcgggtgga gagcgcctga
      481 acgatgaagt gcacccccac ttgcttgccg tggagataac ccgtggcgat ggaaaggaac
      541 gtggcccaca cgaagaagat catggccatc tcgtcggccc acgccaggga gttgttcaac
      601 gcgtaacgga agaagacgcc caacgccatc acggctgcaa agccagcggt ggtgacaacg
      661 taggcgactt cactcagacg atccgcggca tcgctcaggg cgacggcggg tcgaccccag
      721 ctcgacatct cggtcgcgaa cggagttgtg ataccgcgag cctctatggc ttccatatcc
      781 cccctcctgt cactagcgag gcgaagcacg ccgcctcgac cgttgccccc cccttgcgtg
      841 cagcggcgac cgctctcgtc ggcgtgggag cggtgccgag aagatcctga gtcgcggtga
      901 tggcgccacg cgtacactgc gtcgccgggg gtcggcgcgg gctccgatcc gcgtgtccgt
      961 ctagcgcgga ttgagcttga agatctcgtt ggcgatgccg aggtcgatct tgcccttgaa
     1021 ctggtcggcg accacttgcc agaccgtctc ccggacagac atccactgcg ccatctcttc
     1081 tttgtttgga tggtagatgc ggacccccgc tttctccact tcgccgatgg cggccttcac
     1141 cttctcccgt ccatactgcc cggcgagcgc ttcggtctgg gctgcggcgt ccaggagcgc
     1201 tttttgctgg aagggggtga gcgactcgaa cttcttctgg ttcatgtaga tgggaaagag
     1261 gttgttccgg aagcctatgg ctgcgtagta cttggtaact tcgtatagct tgatggacgc
     1321 gtagggcggc aggggggtcc cggcgttgcc gtcgaccaca ccctgctgga gcgcggtgta
     1381 gagctggccc caatccacgg gagtcggatt ggcgccatag gcctttacca cggcaatctc
     1441 gacgggagtg ctggtggagc gcagcttcag gcccttgatg tcggccggga ccctccccgg
     1501 cgtgtcgcgc gtttgcaggt cggtgccgct gacctcggcg tcgtaggagt ggctcgtgac
     1561 cagcagcgtc ttgacgccta ggtctttttc gaactgggcc agcgccttct tcccggcggg
     1621 atggctcttc agcatgtcga tataggctcg atcgttcttg aagaggaacg ggaggtcgaa
     1681 ctggagaaag gcatccgtga aacgagccac gttggatgtt gagatataac cgatatctac
     1741 gctgccgcct ttcacggccg ccgccaactg ctgctccacc cccaaggatt ggtagaacgg
     1801 ttggacgttc aactccccgt tgctcagctt gttcgccagc tcgcacaact tcccgatggc
     1861 catggccggc gcggtcgtcc cttcaggctc ggaggcgccg gctcgaagga tgaccggccc
     1921 ggccgccagg gccggaggcg cagcgcagac cacgagcgcg cagacgaaag cgaaagatat
     1981 ccggggccgc ttcccaaagc acatcttgcc catcgttccg tctccttcat gtggcatggg
     2041 tcatttcggc agggggtagt cgaagaggag gtgagggagc atcagaggga tcatggggaa
     2101 gaccgccatg atcgccaggt cgatgaccat gatgacgatg aacgggatgc aggcccgcac
     2161 cgcgtcctcg tagcgcacgt ctgccaactg cgtcgagacg aagaggttca gcgccacggg
     2221 ggggatgatg cacccgagcc ccatgttggt gcagaacagg actccccagt gcacgatgtc
     2281 cacccccacc ttccccagga gcggcatgaa ggacggcagg aagccgaaga tcatggcggg
     2341 gggctccagg atcatgccga ggatgatgaa caccaggctc accaccagga tcaccaccag
     2401 ccgattggtg ctgatctgcg tgacgaattc caccacgtgc tgggacacca ggtcccgcgc
     2461 catcacaaac ccgaggaacg acgaagtgga ggccatgaaa agcgtcatcc cgctgatgta
     2521 gccggcgtcc accgcagcct tgtagagccg tccgggctgg gccgtcttgt agaagaagga
     2581 cagcaccagc acgtacgcca gaagcacggc tcccacctcg gccggcgtga agaccccgcc
     2641 gaggatcccg ccgagcacca ggacgagcgc gaacatcccg ggcagggctc tcaggatgcg
     2701 tggcagaatg gaatcgcgcg gcacgtactc ccgggggtag ttgtagcgtc gcgcgtggac
     2761 cagggtgacc agcatgaagc ttccggccag caggaaggcc ggcagaatgg tggccgcggc
     2821 cagccggctg accgaggcat tggtggcgct ggcgtacaag aggatggtga tggccagggg
     2881 cgccatcatc cccagggtcc cggcggttcc ctggagcgct gcgcagaagt cggcccggta
     2941 cccccgcgcc ttcatctggg ggatcatcag cgagccgatg gccgccgtgt cggagacggc
     3001 ggagcccgag atgtccccga acaggaagct cgccaccacg ttggccgccc cgagtccccc
     3061 gcggaatcgc cccaccagca cctgggcgaa gtccacgatg tactgggcga tgcccgcctc
     3121 gtgcatcagc tttccggcca ggatgagcag cgggatggcc atcagcgcca gcaggctcat
     3181 cccgttgtaa atctgctggg cccccacatc gaagggggcg tccccgaagg cgccgatgta
     3241 gaaggtggcc atgaagccca gtgacagcga caccggcacc ccgagcagca tgggcccgaa
     3301 cagggcgacg atcaacacgg cggcccgagg caggccggtc agttgcacgt actgtccgat
     3361 gggcagcatc accagaaaca ggaacgtccc cgcgatcagc agcttgaccg cggctgcggt
     3421 tcggatgccg gtcaggagat tacggcgcac ccagtggagg tccatgatca agcaggacac
     3481 cggaatggcc acggcgggga tcgtcagcgg ccagcggagc gcgtcggtgt gcatctggct
     3541 cgccagcggg agggcctgga tgccggagac cgtcagcgac accaggtagg cgagggacag
     3601 gccctccgcc agcaccgtgg cgcgggcttc ccaggccggc ggcagcttct gcaccaggat
     3661 gtccaggttc acatgccggt cgtggaggta gcccgtggcg atgaagagga gcgtggccca
     3721 catgaacacc atgagcgcga cctcgtcgga ccacgccagg gagttgttca acacgtaacg
     3781 gaagaagacg cccaccagca tgatggcgcc gaagccggcg gtggccatca cggcgccgaa
     3841 ggtgcaggcc cggtccacgg catcgcttag ggcgacaacg gatcgacccc agacggacga
     3901 ggtaacggca agcggggtcg tggtgccagc ctccagtttc gcccccattc cttcttctcc
     3961 tcttcctgcc gagtgaagac agcctcgaaa gccaggactt cggggagggc gcgtggtctc
     4021 ctctgcgcca tccccgagat ccgagtgatg ttgcggcggg tttcggcgta tcttacgcgc
     4081 cgcccgactt gcctagcgtg ggatcgactt gaagacgtcg ttggcgacct tgaggtcgac
     4141 cttgcccttg aactcgtcgg ccacctgctg ccagaccttc tcccggacgg acatccactg
     4201 cgccatctcc tcttttatgg gatggtagat gtggacgcca ttcttctcga ccacatcggc
     4261 agcaagcttg acccgctccc gcgcccactg cccggcgagg ggtttggtct gggccgcggc
     4321 atccaggagg gccttctgct ggaagggcgt gagggaatcg aacttcttcc gattcatgta
     4381 gatgggaagc aggttcatcc ggaagcctat ggccacgtag tacttggtga tctcgtacag
     4441 cttgatggcg gcgtagggag gcaaaggagt cgctgcgttg ccgtccacca caccctgctg
     4501 aatcgcggaa tagagctggc cgtagtcgac cggcgtggga ttggcgccgt aggcctttac
     4561 caagaactgc tcgacggggg tgctgccggt gcgcagcttc agccccttga tgtcggccgg
     4621 gaccttcacc ggcttgttcc gtgtctgcag gtcggtgccg ctgaagtctg cgtcgtaggc
     4681 gtggctcgtc cacagcagcg ccttcacccc caggtccttt tcgaactggg tcactgcatt
     4741 cttccc
//
LOCUS       DBNGZC010000374         2974 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1498316, whole genome shotgun sequence.
ACCESSION   DBNGZC010000374 DBNGZC010000000
VERSION     DBNGZC010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1498316"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="AC16G0_06655"
     CDS             complement(<1..721)
                     /locus_tag="AC16G0_06655"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5099340.1"
                     /translation="MRVKILITGGAGFLGSHLTDAFLARGDDVTILDIISDLKVRHLL
                     GSPRFHYVRDSVFNTEILDSLILKCDLVYHLAAVVGVEHYVGDPYEVLNVNINGTQAV
                     LKLAYKYNRKVVFSSTSEVYGRNPKVPFAEEDERVLGSTRIDRWCYSTSKAAAEHFCF
                     AYHRLGLPVVILRYFNVYGPRLDKIDVGRVITIFMGQVLRGEPVSVIGDGRQTRCFTY
                     VSDAIRASVEAGINERAVGQIF"
     gene            complement(712..1671)
                     /locus_tag="AC16G0_06660"
     CDS             complement(712..1671)
                     /locus_tag="AC16G0_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HIR5099341.1"
                     /translation="MFGYHDMGIICLQELLDQQANIVGVVTHEDDPAEAIWFGSVAAW
                     ARTHAIPVLTPEDPRAPAVVEEIGALRPDLLFSFYYRRLLPASILALPKLAAVNLHGS
                     LLPKYRGRAPLNWVLVRGETETGVTLHHMDEQADHGDIIGQRQVPIGVEDTALTLWRK
                     LTAAGRLLVAELYPQIVTGRASRIPQDHSRATTFGRRRPADGLIDWSRSAWEVYNLIR
                     AVTHPFPGAFTFWEHRRIFLWGARPPRAGEAQGPPGTILGQGQDGALRVATGQGVLEV
                     VRLQPEGDAEQAGASFVTTLGRNLETRFSSPASQAAGDRGKCA"
     gene            complement(1789..2787)
                     /locus_tag="AC16G0_06665"
     CDS             complement(1789..2787)
                     /locus_tag="AC16G0_06665"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5099342.1"
                     /translation="MTEVGSQGSEVAAPISLPEGGRAQRPEPAISVVIPVYNEEANLE
                     PLYERLDRVLKGTGRAYEVIFVDDGSRDRSLAILRRFQEKYEQVRVIQLNRNYGQHAA
                     VFAGLDHARGAVIVTLDADLQNPPEEIPRLLDKIAEGHDVVGGWRQDRQDHAVRRAFS
                     WLVNKLTSRLVGVRMRDYGCMLRAYRREVVEVLKGCGELSGFVPALANTFAGSPTEVP
                     VGHDRRSVGRSRYSPFRLIRLNFDLMTGFSLFPIQCVSLAGIGISLLGLAFGGYLMLR
                     RLFLGPELEGVFTLFAILFVFVGIQILALGLIGEYIGRIYLEVRRRPRYVVQRIYE"
BASE COUNT          528 a         1005 c          966 g          475 t
ORIGIN      
        1 tgaagatctg gcccacggcc cgctcgttga tgcccgcctc cacgctggca cggatggcgt
       61 cgctcacgta ggtgaagcaa cgagtctgtc tgccatcgcc gatcacgctg accggctcgc
      121 cccggagcac ctggcccatg aagatggtga tgacgcgacc cacgtcgatc ttgtccagac
      181 gtggaccgta gacgttgaag tatcgaagga tcaccacggg cagccccagg cgatggtagg
      241 cgaagcagaa gtgctcggcc gcggccttgg aggtggaata gcaccaccga tcgatgcgcg
      301 tggaacccag cacgcgctcg tcctcctcgg caaagggaac cttggggttg cgcccgtaca
      361 cctccgaggt ggagctgaag accaccttcc ggttgtactt gtacgcgagc ttcagcaccg
      421 cctgggtgcc attgatgttc acgttgagca cctcgtaggg atcgcccacg taatgctcca
      481 cccccaccac cgccgccagg tgatagacga gatcgcactt taggatcagg ctgtccagaa
      541 tctcggtgtt gaacaccgaa tcccggacgt aatggaagcg gggactgccc agcaggtggc
      601 ggaccttcag gtcgctgatg atgtccagga tggtgacatc gtcgccccgc gccaggaagg
      661 catcggtcag atgggatccc agaaagccag ccccgccggt gatgaggatc ttcacgcgca
      721 tttccccctg tccccggccg cctgcgaggc cggcgacgaa aaccgtgtct ccagattcct
      781 tccgagggtg gtcacaaagc tggccccagc ctgctctgcg tcgccctccg gctgcagccg
      841 cacgacctcc aggaccccct gtcccgtggc cacccggagg gcaccgtcct ggccctggcc
      901 aaggatggtc ccgggcggtc cctgggcctc cccagcgcgg ggtggtcggg ctccccacag
      961 gaagatccgc cggtgctccc aaaatgtgaa ggcccccgga aacgggtggg tcaccgcgcg
     1021 aatcaggttg tacacctccc aggccgatcg ggaccagtcg atcaggccgt ctgcgggccg
     1081 ccgccggccg aaggtcgtcg cccgcgagtg atcctgaggg atgcgtgacg cccggcccgt
     1141 gacgatctgc gggtacagct cggccaccag caggcgcccg gcggcggtca gcttgcgcca
     1201 gagcgtgagg gccgtatcct cgaccccgat cggcacctgc cgctggccaa tgatgtcccc
     1261 gtgatcggcc tgctcatcca tgtggtgcaa ggtcacgccg gtttcggttt ctccccgcac
     1321 cagcacccag ttgaggggcg cgcgcccgcg gtatttcggg agcagggagc cgtgcagatt
     1381 caccgccgcc agcttcggca gggccaggat ggaggccggc agaagccggc gatagtagaa
     1441 cgagaagagg aggtccggcc ggagcgcccc gatctcctcg accaccgcgg gcgccctggg
     1501 gtcttccggg gtgagcaccg gaatggcgtg cgtccgggcc caggcggcca ccgagccgaa
     1561 ccagatcgcc tccgccgggt cgtcctcatg cgtcaccacg ccgacgatat tcgcctgctg
     1621 atccagcagt tcctggagac agataatccc catgtcatga tagccaaaca cgacgacgcg
     1681 cggtgattcg gccggcccag gtgtgggccc cggtctgctc agacgctctt ggctgtccac
     1741 gagtgatccg gtcaaaggcg cgggggaacc aggaggcgtg ccgtgcggct attcgtagat
     1801 gcgctggacg acatatcgcg gcctccgccg cacctccagg tagatccggc cgatgtactc
     1861 gccgatgaga cccagggcca ggatctggat gcccacgaac acaaagagga tggcgaaaag
     1921 ggtgaacacc ccctccagct cgggtcccag gaacagtcgc cgcagcataa ggtatccacc
     1981 aaaggccagg cccagcaggg atatcccaat acccgcgagg ctgacgcact ggatgggaaa
     2041 cagggagaac cccgtcatga ggtcgaagtt gagccggatg aggcggaacg ggctgtacct
     2101 ggaccgcccc acgctccgcc ggtcatgccc caccgggacc tcggtgggtg atcccgcaaa
     2161 ggtgttggcc agggcgggga caaagccgga cagttcgccg cagcccttga ggacctcgac
     2221 cacctcccgc cgataggcgc ggagcatgca gccgtagtcc cgcatccgga ccccgacgag
     2281 ccgggaggtg agtttgttta ccagccacga aaaggcgcga cgcacggcgt ggtcctggcg
     2341 atcctgacgc cagccgccga ccacgtcgtg gccctcggcg atcttgtcca ggaggcgcgg
     2401 gatctcctcg ggcggattct gcagatccgc gtccagggtg acgatgaccg ccccccgggc
     2461 gtgatccagc ccggcgaaga cggccgcgtg ctggccgtag ttccggttga gctgaatcac
     2521 gcggacctgc tcgtatttct cctggaaacg acgcaggatc gccaaggacc gatcccggct
     2581 gccgtcgtcc acgaagatca cctcgtaggc gcgacccgtt cccttcagca cccggtccag
     2641 ccgctcgtag agcggttcga gattggcctc ctcgttgtag acggggatga cgactgaaat
     2701 cgccggctcg ggccgctgcg ctcttccgcc ctcgggcaag ctgattggcg cggccacctc
     2761 ggaaccctgg ctgccgacct ccgtcactcc tcacctcgtg ttctgggcac cggtcggatg
     2821 ggcggccatg accacgctca ttccgacccg cgtctcggct gccatgccag ctctggatgg
     2881 cgagagggca acccgcgcgt cgagcccgcg gaatagtagt cgggattgac ccacgagtca
     2941 agttgcggcg gcttcgcttg cgggggcttc gccg
//
LOCUS       DBNGZC010000375         2150 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_325977, whole genome shotgun sequence.
ACCESSION   DBNGZC010000375 DBNGZC010000000
VERSION     DBNGZC010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_325977"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="AC16G0_06670"
     CDS             <1..350
                     /locus_tag="AC16G0_06670"
                     /inference="COORDINATES: protein motif:HMM:NF014073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR5099343.1"
                     /translation="PKKVLLPLVMLFCLTGMYSVNNFLVDLWVMLACGALGYLMRKWA
                     YEGAPLLLALVLGPRMEVAFRQSLMISHGDFAVFLARPISLVFLLATLGFLLMPLLKR
                     GVRSVTRKAPAAK"
     gene            385..1338
                     /locus_tag="AC16G0_06675"
     CDS             385..1338
                     /locus_tag="AC16G0_06675"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR5099344.1"
                     /translation="MRTIIGVVLGLTLALGIPGPGLAADFPTKEVQIIIPWAAGGATD
                     LVFRALAASSSKYLGKAVVVVNKPGGGGAVGYTEVAQARPDGYTLVTAVTPLTILPHQ
                     TKTAFTYQSFAPVINVVEDPAMFQVRADSPWKTLPEFLDYAQKNPEMITVGNSGAGGG
                     VHLIAMAFEKAAGVRFNHIPFAGGGPSVTALLGGHVHAVSVSPPEGIAHVRAGKLRII
                     ALFSDKRMEAFPSVPIVREQGINFSMGQWRGLAAPKGTPPDVINRLHEAFKKGMEDPT
                     FVKNATDMTVHLAYSGPAEFGKIMAQDHERYAKLVPEIKKQ"
     gene            1381..>2150
                     /locus_tag="AC16G0_06680"
     CDS             1381..>2150
                     /locus_tag="AC16G0_06680"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR5099345.1"
                     /translation="MNTVRWVPILLTLTLAVVVAGCAETMQTAAPGGAKTVVRIASPF
                     KPGTILVDAAQKFKELVAQGSSGRIDVQIDAGTKSEEAINKANRTGEIEMQSNGTMFL
                     QDYAPPYYFFTGPYVMKDFEHYMRVWNGKLGQAARAQLEKNDLKYLATIYRGLRQMTT
                     KKPVYTPADVYGLKLRLPNIPTWMAVWKAMDANPIGVPLTELYNSLKTGKAESSEGDL
                     PQISGFKLDEHEPRARCTTNSLRTCPASFEGTSAVTST"
BASE COUNT          398 a          692 c          684 g          376 t
ORIGIN      
        1 tcccgaagaa ggtcctgctg cccctggtga tgctcttctg cctcaccggc atgtacagcg
       61 tgaacaactt cttggtggat ttgtgggtga tgctcgcctg cggggccctg ggctacctga
      121 tgcgcaaatg ggcctacgag ggagcaccac tcctgctggc gttggttctc gggccccgga
      181 tggaggtggc cttccggcaa agcctcatga tctcccacgg ggactttgcc gtttttctgg
      241 cgcgtcccat ttcgctagta ttcctcctgg ccaccctggg atttttgctc atgccgctcc
      301 tgaagcgcgg cgtgcggtcc gtcacgcgga aggcgcccgc cgcgaagtag tctgcaatcc
      361 atcggagggg gaaggagggc tctcatgcgt accatcattg gcgtcgtcct gggtctgacc
      421 ctggcgctcg gaatccccgg gccggggctg gccgccgact ttccgaccaa ggaggtgcag
      481 atcatcatcc cctgggcggc cggcggggcc accgacctgg tcttccgcgc cctggccgcc
      541 agcagctcaa agtaccttgg caaggccgtg gtcgtggtga acaagcccgg tggcggcggg
      601 gccgtcgggt ataccgaggt cgcgcaggcg cgccccgacg gctacaccct cgtcacggcc
      661 gtcacgcccc tgactatcct gccccaccag accaagacgg ccttcaccta tcagagcttc
      721 gcgcctgtga tcaatgtggt cgaggatccg gccatgttcc aagtccgtgc cgactccccg
      781 tggaagacgc ttccggagtt cctcgattac gcccagaaaa atcccgagat gatcacggtg
      841 ggcaactcgg gggcgggggg cggcgtccac ctcatcgcca tggccttcga gaaggctgcc
      901 ggtgtcaggt tcaatcacat cccctttgcc ggtggtggtc cctcggtgac ggccctcctc
      961 ggcgggcatg tccatgccgt ctcggtttct ccgccggagg gcatcgcgca cgtccgcgcc
     1021 gggaagctcc ggatcatcgc cctgttctcc gacaagcgaa tggaggcctt ccccagcgtg
     1081 ccgatagttc gcgagcaggg gatcaacttc agcatggggc aatggcgtgg cctggcggcg
     1141 cccaagggaa cgccgccgga cgtcattaat agactgcacg aggcgttcaa gaaggggatg
     1201 gaagacccga ccttcgtgaa gaacgccacg gatatgacgg tgcacctggc ctactcgggc
     1261 ccagccgagt tcgggaagat tatggctcag gaccacgagc ggtacgccaa gcttgttccg
     1321 gagatcaaga agcagtagcg gggcggggag tcccgcccgg aggagaggag ggcactgacc
     1381 atgaatacgg tacgatgggt cccgatcctt ctcaccctga cgctggcggt cgtcgtcgcc
     1441 ggctgcgcgg agacgatgca gacggccgcc cccggcggtg ccaagacggt cgtccgcatc
     1501 gccagtccct tcaagcccgg caccattctg gtcgacgctg cacagaagtt caaggagctg
     1561 gtggcccagg gcagcagcgg ccggatcgat gtgcagatcg atgccggcac caaatcggaa
     1621 gaggccatca ataaggccaa ccggacgggt gagatcgaga tgcagtccaa cggaaccatg
     1681 ttcctgcagg actacgcccc tccgtactac ttcttcaccg gcccgtacgt catgaaggac
     1741 tttgagcact acatgcgtgt ctggaacggc aagctcggcc aggcggcgcg ggcccagttg
     1801 gagaagaacg acctgaaata cctggcgaca atctatcgag ggctgcggca gatgacgacc
     1861 aagaagccgg tctacacgcc ggcggacgtc tacggactga agctgcggct gcccaacatc
     1921 cccacctgga tggcggtctg gaaggccatg gacgcgaacc ccattggcgt cccgctaacc
     1981 gaactctaca attcgctcaa gacgggcaag gcggaatcct cggagggcga cctgccccag
     2041 atctccggtt tcaagctgga cgagcacgag ccacgagcac ggtgcacgac caattccttg
     2101 cgcacctgcc cggccagctt cgaagggacc agcgccgtca catcgacgtg
//
LOCUS       DBNGZC010000376         2448 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1302381, whole genome shotgun sequence.
ACCESSION   DBNGZC010000376 DBNGZC010000000
VERSION     DBNGZC010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1302381"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="AC16G0_06685"
     CDS             complement(<1..253)
                     /locus_tag="AC16G0_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099346.1"
                     /translation="MARRGVPAFEEMALAAWVAEGEYPAIPAGIRAHAVRIFQDTLGV
                     ILRGAEASEVRRLASAMPKVARGGATILTQGFPRSDPFTA"
     gene            complement(323..1918)
                     /locus_tag="AC16G0_06690"
     CDS             complement(323..1918)
                     /locus_tag="AC16G0_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR5099347.1"
                     /translation="MEPSEETRRWISQVVGIPVDEHLARSREAWATLGPALAGVQEAP
                     VPEETEPAVQMGFPPGEEPPDARSPDQEDGEAEPAAANQPWELTLTDAGDMLRTGRLS
                     TVELVRSVLDRISVTEPHVLAWAHMQPEAALAQAERSDRLLRQGTYLGPLHGIPLGIK
                     DVYFTAGIETAAGSRALRGFVPSFDATVVRRLREAGAIILGKTATTEFAYADPAATRN
                     PWNPAHTPGGSSSGSAAGLAAGMCLGALGTQTGGSIIRPAAFCGVVGLKPTYGRSSRH
                     GLIPLAWSLDHPGPMARTVRDIARLLGAMAGPDVHDPSAAMRPVPVYVKCLRLAPRGL
                     TAGIPDRYFLERSDPEVAAAFHEAVRVLEGLGMRPHEVKLPDVFEAGVCAGRVIMHAE
                     GAAVHRDRFKTRWEDYRPKLATLVEAGLLIPAATYLRAQQVRSLAIGAMRRLLSDVDV
                     ILTPSTPCPAPEGLASTGDSVFNSPASTFGLPALGLPMGFAPSGLPLGLQIMGRPFDE
                     CTLLRVGAAYEAATPWHGQRPAL"
     gene            complement(2028..>2448)
                     /locus_tag="AC16G0_06695"
     CDS             complement(2028..>2448)
                     /locus_tag="AC16G0_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006233628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR5099348.1"
                     /translation="IGWFTRPMQTLNTRRILAEEGFLYDSYALNDDLPYFQEVAGKPF
                     LIVPYTLDQNDTRFQKNQMFTGDDFFFYVRDAFDTLYEEGETHPKMMSVGLHCRLIGR
                     PARALALDRFLAHVRQHKDVWIANRNEIARFWMKTHG"
BASE COUNT          392 a          828 c          830 g          398 t
ORIGIN      
        1 cggccgtgaa gggatccgat cgcgggaatc cctgcgtgag gatggtggcg ccacctcgcg
       61 ccaccttggg catggcagat gccaggcgcc gcacctccga ggcctcggcg ccgcgcagga
      121 tcacgcccag cgtgtcctgg aagatgcgca cggcgtgggc ccggatacct gccgggatgg
      181 cgggatactc accctcggcc acccacgcgg ccagggccat ttcctcgaac gctggaaccc
      241 ctcgacgcgc catcgcccct cctctcgtgc gcctccgatc attctccctg acggccgtgc
      301 cgtccggcgg cgtctggtta tctcagagcg caggtcgctg tccgtgccac ggcgtggcag
      361 cctcataggc cgcacccact cgaagcagcg tacactcgtc gaagggtcgg cccattatct
      421 gcagtcccag ggggagcccc gagggtgcaa aacccatcgg gagacccagc gccgggagtc
      481 cgaaggtgct cgccggcgag ttgaacaccg aatcccccgt ggaggcgagg ccctccgggg
      541 cggggcacgg cgtagacggg gtgaggatca catccacgtc gctcaagagc cgccgcatgg
      601 ccccgatggc cagggagcga acctgttgcg cgcggagata ggttgcagcc gggatgagca
      661 gaccggcctc gaccagcgtg gccagcttcg gtctatagtc ctcccatcgc gttttgaacc
      721 gatcccggtg tacggccgca ccctcggcgt gcatgatgac gcggccggcg cagacacccg
      781 cctcgaagac gtccggcagc ttcacctcgt gcgggcgcat accgagccct tcgagcaccc
      841 gcacggcctc gtgaaacgcg gccgccacct ctgggtcgga tcgctccagg aagtaccggt
      901 cgggaatccc ggcggtgagg ccgcgcgggg caagtctcag gcacttcaca tacactggca
      961 cgggccgcat cgcagcactc gggtcgtgga catccgggcc cgccatggcg ccgagcagcc
     1021 gggcaatgtc tcgcaccgtc ctggccatgg ggccggggtg gtcaagggac caagccaggg
     1081 gaatgaggcc gtggcgactg gaccgtccgt aggtaggttt cagccccacc acgccgcaga
     1141 acgcggcggg tcgaatgatg gaacctccgg tctgggtgcc gagcgccccc agacacatgc
     1201 ccgcagccag ccccgcagcc gagccgctgc tcgacccgcc gggggtgtgt gccgggttcc
     1261 acgggttccg cgttgcggcc gggtcggcat aggcgaattc cgtcgtggcc gtcttgccca
     1321 gaatgatcgc gccggcctcc cggagtcgcc ggaccacggt ggcatcgaag ctcgggacga
     1381 atccacggag cgcccgagat cctgcggcag tctcgatccc cgccgtgaag tagacgtcct
     1441 tgatccccag cgggattccg tggagcggtc cgagataggt gccctggcgg aggagtcgat
     1501 cggaccgctc ggcctgggcc aaagccgcct ccggctgcat gtgtgcccag gccaggacat
     1561 gcggctccgt gacggagatc cgatccagga cggaccggac cagctcgacc gtcgaaaggc
     1621 ggccggtccg gagcatatcc cccgcgtcgg tgagcgtcag ctcccacggc tggttcgccg
     1681 ccgccggctc agcctctccg tcctcctggt ccgggctcct ggcgtctggc ggctcttcac
     1741 ccgggggaaa ccccatctgg acggcgggct cggtctcctc tggtacaggg gcctcctgca
     1801 cgcctgccag ggcagggccg agggttgccc aggcctctcg agaccgcgcg aggtgctcgt
     1861 ccacgggtat ccccaccacc tggctgatcc agcgacgtgt ttcctcggat ggttccatgc
     1921 ggatctcctg ggactccccg cagtctgtcg caggcgatca gatcggtcag gtgccctcag
     1981 gctgaatatg gactggacgg gaaccgcgtc ggcgctctcg cctttgccta cccgtgcgtc
     2041 ttcatccaga agcgagcgat ctcgttgcgg ttggcaatcc acacgtcctt gtgctggcgg
     2101 acgtgggcga ggaagcgatc cagggccagg gcccgcgccg gccgcccgat caggcggcag
     2161 tgcagcccca ccgacatcat cttgggatgg gtttccccct cctcgtagag ggtgtcgaag
     2221 gcgtcccgga cgtagaagaa gaagtcgtca ccggtgaaca tctggttctt ttgaaagcgg
     2281 gtgtcgttct ggtcgagggt gtaggggacg atcaggaatg gcttgcctgc cacctcttga
     2341 aagtatggaa ggtcgtcgtt caaggcgtag ctgtcgtaca agaaaccttc ctccgccaag
     2401 atgcggcggg tgttcagggt ctgcatgggg cgagtgaacc agccgatg
//
LOCUS       DBNGZC010000377         3174 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_519409, whole genome shotgun sequence.
ACCESSION   DBNGZC010000377 DBNGZC010000000
VERSION     DBNGZC010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_519409"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..2755)
                     /locus_tag="AC16G0_06700"
     CDS             complement(215..2755)
                     /locus_tag="AC16G0_06700"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5099349.1"
                     /translation="MIEAKRTQAVLTEGEEKYRQLFATVSDAIVVFDAETRRFVDVNE
                     RALRLYGYTRDEFLSLTHGDITAEPAASEASIRETLSGMGTGVPLRYHRKKDGTVFPV
                     EISAGAFVWEGRSAVCGVIRDITDRKRVEAALWQSEVSLNRAQQVAHIGSWHLDVIRG
                     ELTWSEETYRIFGVAIATPVTYERFLETVHPDDRESVAKAWAAALTGEPYTIEHRILV
                     AGETKWVRENAEVEFDEDGTALRGIGTIQDITDRRQAEDALRVRTQQLEALRAVGDEI
                     TRELDLPTLLNLIMHRSTELVGGASGTIYLWDEAEQALIPRIWATHGEWMREGRVEMG
                     EGLAGLVAARHEGLIVNDYRTWPHARPFILDQTNITAVVAEPLLYRERLLGVIAVDDQ
                     GTGRRFTEQDRELLRLFAIQAAVAIENARLFAATEQRAAQLKTLRDIGQAIAARLELP
                     EVLEAVVSGTMELLGGSHAEITLWDEATRRLRHGAAGGTQATMGGTDEIALGEGVMGM
                     VALTWQPLVINDYQASRYASADCQDIRATITVPVLFEDRLLAVLHAHTTRPGKSFTAN
                     DRRRLQMVATQTAIAIENARLYEQVREYANELERKVEERTQELQAANTQLRAANAELE
                     AFSYSVSHDLRTPLLSIKGFSRVLLKKYERVLGAEGMDYLRRVQTGAQRMGEIIEALL
                     GLARVTRHPLQQEPVDLSAQARTIVNDLRQRDATREAEVIIQDDLVVRGDARLLCTLL
                     ENLLWNAWKFTANRRPARIQVGRCSGEGGAAVYFVGDNGVGFDMAEADKLFGMFQRLQ
                     GAEQFPGTGIGLATVQRIVERHGGRVWAEGRIEGGATFFFTLSPLAGS"
BASE COUNT          526 a         1055 c         1000 g          593 t
ORIGIN      
        1 gggcggcggg ggtccggccg ccacagggta gccgcaggtt tggcaccatc cttggagccc
       61 agcccccgct gggccgggtt cgaggggttg gaccgagcgg cagcagcgac agaacgccaa
      121 actggtcatg aacagaatct cctcctcgcc tctcgggatg ccggcacccc ccgcagccgg
      181 acttccacga gcagcccagg cgccgacgag tctttcagct tccggcgagc gggctgaggg
      241 tgaagaaaaa ggtggctccc ccctcgatcc tcccttccgc ccacacacga ccgccatgtc
      301 gctcgacgat ccgctggacg gtggcgaggc cgattccggt tccggggaat tgctcggcac
      361 cctgaagacg ctggaacatt ccgaagagct tgtcggcttc cgccatatcg aaccccacac
      421 cattgtcacc cacgaaatac actgccgctc ctccctcccc ggagcaccgc ccgacctgga
      481 tgcgggccgg ccgtcgattc gcggtgaact tccaggcgtt ccagagcaga ttttcgagga
      541 gggtgcagag cagtcgagcg tcgccccgga cgacgaggtc atcctgaatg atcacctcgg
      601 cctcacgcgt cgcgtcgcgc tgcctcaggt cattgacgat ggtccgtgcc tgggcgctga
      661 ggtccactgg ctcttgctga agcgggtggc gggtcacccg cgccaagccc aggagggcct
      721 cgatgatctc tcccatgcgc tgcgcccccg tctgcacgcg tcgcaggtag tccattccct
      781 ctgcacccag gacccgctcg tatttcttca ggaggacccg gctgaagccc ttgatgctga
      841 gcaagggggt gcgaaggtcg tgcgacacgg agtatgagaa ggcctccagc tctgcattcg
      901 cggccctcaa ctgggtgttg gcggcctgga gttcctgcgt cctctcctcg accttccgct
      961 ccaactcgtt cgcgtattct ctcacctgtt cgtacagccg tgcgttctcg atggcgatgg
     1021 cggtttgagt cgcgaccatc tggagccggc ggcggtcatt cgccgtgaac gatttcccgg
     1081 gccgggtggt gtgggcgtgc aggactgcga ggagtcgatc ttcaaacaga acggggacgg
     1141 tgatggtggc gcggatatcc tggcagtcgg ccgacgcgta gcgtgacgcc tggtagtcgt
     1201 tgatgaccag aggctgccag gttagcgcga ccatccccat gaccccctcg ccgagggcga
     1261 tttcatcggt cccacccatg gtcgcctggg tgccacccgc cgccccgtgc cgaagacgac
     1321 gggttgcctc gtcccagagg gtgatctcgg catgtgagcc acccaggagc tccatcgtcc
     1381 ccgacaccac cgcttcgagg acctcgggga gttccagccg ggccgcgatg gcctgcccga
     1441 tgtcccggag ggtcttgagc tgggcagccc gctgctcggt ggccgcgaag agccgggcat
     1501 tctcgatggc gacggccgcc tggatagcga atagacgcag gagctcgcga tcctgctccg
     1561 tgaagcgccg accggtgccc tggtcgtcga cggcgatcac cccgaggagt cgctcccggt
     1621 agaggagggg ctcggcgact acggccgtga tgttagtctg gtccaggatg aaaggccgcg
     1681 cgtggggcca ggttcgatag tcgttgacga tcagcccctc gtgtcgcgct gcgaccaggc
     1741 cggcgagtcc ttcgcccatt tccacccgtc cctcgcgcat ccactcgccg tgcgtggccc
     1801 agatccgggg aatgagggcc tgctcggcct cgtcccacag gtagatcgtc ccgctcgctc
     1861 cccccaccaa ttccgtcgac cggtgcatga tgagattcag gagggtcggc aaatcgagtt
     1921 cccgggtgat ctcgtcgcca acggcccgga gggcctcgag ttgctgggtg cggactcgca
     1981 gcgcatcctc ggcctgcctg cggtcggtga tgtcctggat cgtgcctatg ccgcgaaggg
     2041 ccgtcccgtc ctcgtcaaat tctacttcgg cattttccct gacccacttc gtttctcccg
     2101 ccaccaggat gcgatgctca atcgtatacg gctcgccggt cagcgcagcg gcccaggcct
     2161 tggctacgga ttccctgtca tcgggatgaa ccgtctccag gaagcgttcg tacgtcaccg
     2221 gggttgcgat cgcgacgccg aaaatgcggt aggtctcttc ggaccaggtg agttcccccc
     2281 tgatcacgtc caggtgccag cttccgatat gggccacctg ctgcgcacga ttcagactga
     2341 cctcgctctg ccagagcgct gcctctaccc gcttgcggtc ggtgatgtca cggatgacgc
     2401 cgcacaccgc gctccggcct tcccacacga acgccccggc ggagatttcc accgggaaca
     2461 ccgtcccgtc cttcttcctg tgatagcgga ggggcacccc ggtacccatc ccgctcaggg
     2521 tctctcggat cgatgcctcc gatgcggctg gttcagcggt gatgtcgccg tgggtgagac
     2581 tcaggaattc gtcgcgggtg tagccgtaga ggcgcagggc gcgctcattc acgtcaacga
     2641 accgccgcgt ctcggcgtca aagacaacga tggcatccga gacggtggcg aagagctggc
     2701 ggtatttctc ctcgccttct gtcagcaccg cctgggttcg cttggcctcg atcacggcat
     2761 gctccaactc tgcgcagcga cgccgtaagg tctcgagttc gatcagccag gactcctggc
     2821 tgtcatcccg atcaactaga tcgatcctct tccgaccgcc gtttccctca ggcgatgaat
     2881 gcgacagggg actgaagcct ctcgctcggg tagccatgaa tctgggaaca gacgacgctc
     2941 cgaagattca agaaactgac gaaaacaggc cagctccgtg cagcccgtcc tgcgcggaac
     3001 gtgccgtggc gggcgagcac tcccgctatt gtggaagcgg catggggatg ctcgggatgc
     3061 gagcgtatcg gaggcgacca gacgtgtcaa cgggaaatgc ggccagcgcc cggtccaaat
     3121 ccagatcgct gtctcggcct tggcaagaat gggattttcg gagtataaag agcc
//
LOCUS       DBNGZC010000378         1555 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2281902, whole genome shotgun sequence.
ACCESSION   DBNGZC010000378 DBNGZC010000000
VERSION     DBNGZC010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2281902"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1478
                     /locus_tag="AC16G0_06705"
     CDS             <1..1478
                     /locus_tag="AC16G0_06705"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HIR5099350.1"
                     /translation="PYLLGTTLAQILDVCNFHLHFELSPGGLVPRWHRLREVLPGLTH
                     QPGMSERSGKERLACIVPSGWLGIGHRLYHRAPGSFDLFRDVTTRTAIPEPTAAFPTQ
                     DSASILDHFAGLSDPRREQGRIHRLDEIVFMATCAVFCGADTWEQIAGYAHSKVDWLR
                     TFLTLPGGVPSHDTFRRVFCLLDPVAFQKCFYAWMTALMERRGLTPLAANPPELTPIA
                     IDGKAQRGSARRTVGQSALHIVSAWAVENHLTLGQVATDAKSNEITAIPELLELLDLE
                     GAVVTIDAMGCQKEIAADIVGEGGQYVLAVKENQPHLYEDIERAFDEVLERGEAGVDF
                     TECQTEGVRRGRQETRICWVITDPKGSRDAGLWTGLTAIVMVISHRVVNGAASGEIRY
                     FIGSATATAAEYLRWVRGHWGIENSLHWVLDVCFREDDQRHWAGNSAQNLAWLRKLAL
                     CLLKAEKNSKGKSIATRRLLAGWKDDYLLSVLAQISEKPGA"
BASE COUNT          274 a          501 c          495 g          285 t
ORIGIN      
        1 tcccttacct cctgggcact acgctggcgc agatcctcga cgtgtgcaac ttccacctcc
       61 acttcgagct ctcccccggc ggcctggtgc cgaggtggca ccggctgcgg gaggtcctcc
      121 cagggcttac gcatcagcct gggatgtctg agcgctctgg gaaggagcgg ctcgcctgta
      181 ttgtgccgtc cggctggcta ggaatcggac accgtttgta ccatcgagcg ccaggatcat
      241 tcgacctctt cagggacgtc accaccagga ccgccatccc cgagccgacc gccgctttcc
      301 cgacccagga ctcggcctcg atcctcgacc atttcgccgg cctttccgat ccccgccgcg
      361 aacagggacg aatccatcga ctcgacgaga tcgtcttcat ggccacctgc gccgtgttct
      421 gcggtgccga tacctgggag cagatcgccg gctacgccca ctccaaggtc gactggctcc
      481 ggaccttcct caccttgccc ggcggcgtcc cctcgcacga tactttccgc cgcgtcttct
      541 gcctgctcga cccggtggcc ttccagaagt gtttctacgc ctggatgacg gcgctgatgg
      601 agcggcgggg attgacccca ctcgccgcca atccacccga actgacgccg atcgccatcg
      661 acggcaaggc tcaacgcggt tcggcgcggc gcaccgtcgg tcaatcggcc ctgcatatcg
      721 tcagtgcttg ggccgtcgag aatcatttga ccttggggca agtggccacc gacgccaaat
      781 ccaacgagat cacggccatc cccgagttgc tcgagctatt ggacctggag ggggctgtgg
      841 tgaccatcga cgccatgggc tgtcagaagg agatcgccgc cgacatcgtc ggcgagggag
      901 gccagtacgt gctggccgtc aaggagaatc agccgcatct ctacgaagac atcgagcggg
      961 cgttcgacga ggtcttggag cggggtgaag ccggtgtgga cttcaccgag tgtcagaccg
     1021 aaggagtccg ccgcggccgc caggagaccc gcatctgctg ggtgatcacc gaccccaaag
     1081 ggagtcggga tgcggggctg tggaccgggc tgacggcgat cgtgatggtg atcagccatc
     1141 gggtagtcaa cggagcagcg agcggcgaga tccgctactt catcggcagc gcgacggcga
     1201 cggcggcgga gtatctgcgg tgggtgcgcg gccattgggg gatcgagaac tcgctgcact
     1261 gggtcttgga cgtctgtttc cgcgaagatg accaacggca ctgggccggc aacagtgccc
     1321 agaatctggc ctggctccgg aagctggcgc tgtgcttgct caaggcggag aagaacagca
     1381 agggcaagag tatcgctaca cgccgcctgc ttgccggttg gaaggacgac tacctattga
     1441 gtgtactggc ccaaatctcc gagaaacctg gtgcgtaagc cctgcttctt ccgatcgaac
     1501 ttccattgga actgccgggg ccctccgttg gtcagggtct cgtagagtcg gagcc
//
LOCUS       DBNGZC010000379         5730 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1205333, whole genome shotgun sequence.
ACCESSION   DBNGZC010000379 DBNGZC010000000
VERSION     DBNGZC010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1205333"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1353
                     /gene="lysS"
                     /locus_tag="AC16G0_06710"
     CDS             <1..1353
                     /gene="lysS"
                     /locus_tag="AC16G0_06710"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HIR5099351.1"
                     /translation="GGRVLSLRGHGKASFAHIQDRTGKIQIYVRQDAVGETAYHLFKR
                     VDVGDFLGVAGHLFRTKTGELTVHVETLTLLAKALRPLPDKWHGLSDVEVRYRQRYVD
                     LIANPPVVELFRRRARIIGEIRRFFDARGFLEVETPMMQPIPGGATARPFMTHHNALD
                     LTLYLRIAPELHLKRCVVGGLERVYEINRNFRNEGLDTQHNPEFTMLEFYQAYADYRD
                     LMVLTEELLSGVVQAVCGGETITYQGRSIGFTPPWPRLSVEEGLVKLGGLQPEQVLTA
                     EGLRAAAEGRGVPLNPAWGWSKLLVELFEVLVEPQLFQPTFVTDFPSELSPLAKARPD
                     DPRYVQRFELYLGGLEVANAYTELNDPREQRRRFEAQVKARAAGDLESHLMDEDYLRA
                     LEYGLPPTAGEGIGIDRLVMLLTDAPSIRDVILFPLLRPEARSAEPSAIEAAPGAPPE
                     "
     gene            1361..2644
                     /locus_tag="AC16G0_06715"
     CDS             1361..2644
                     /locus_tag="AC16G0_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HIR5099352.1"
                     /translation="MALSLPYEVFISLRYLKAKRRQRSVSVISFISTAGVAVGVMALI
                     VVMAVMSGFEFDLRNKILGTNAHIWVLRYGERGIEDPVAALTAIRQVPGVTAASPFTY
                     NEVLLTAGGRTSGAVLRGVDLASAVETTDFRKTLKSAGADLTAAMNTPRPAFPPDGIV
                     LGSILAGTLGVSVGSPVDVLLPLGGDLSPLGVIPRMRRFNVVGVFQTGFAEFDSKLAM
                     IAIPSAQQLFRMGSAVTGIEVRVQDIYQAAGMAEAIRAKLKFPYFTRTWMELNKNFFS
                     ALRVERMVMFVILTMIVLVAAFGIVSTLIMLVMQKRKEIAILKSMGATNRSIMGIFIV
                     QGVIIGIAGTALGLLAGLLIANNLDPIVAAIEWVFGFKAFPQDVYLLDKLPSRVLWPD
                     VIAIGITAFLVSLLATILPSRQAAQIDPVVSLRYE"
     gene            2691..3410
                     /locus_tag="AC16G0_06720"
     CDS             2691..3410
                     /locus_tag="AC16G0_06720"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099353.1"
                     /translation="MSDGIRVQDVTKCFARDGDRVEVLKGVSFDIAPGELVAIVGPSG
                     VGKSTLLHLLGALERPTGGEIRYGETVLSGLSDADLAEFRNRRVGFVFQFHHLLPEFT
                     ALENVMLPLLIRRCPAGQAKERAAVLLGLVGLTERLRHRPGELSGGEQQRVALARALV
                     GEPTVLLADEPTGNLDSKTGDEVFALLRQLNRERRLTSILVTHNETMARQADRILRML
                     DGRIEGEGPTPTAEAPRALQI"
     gene            3520..>5730
                     /locus_tag="AC16G0_06725"
     CDS             3520..>5730
                     /locus_tag="AC16G0_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR5099354.1"
                     /translation="MFERFTERARKVIILAREEAIRLGHNFVGSEHLLLGLIREGDGL
                     AVAILKKLNVSVSALKAEIEKIVAVGTEFSPAGEIPFTPQAKKVLEYAISEARSLGHN
                     YIGTEHLLLGLIREGEGIASLVLRDFGVSVAAAKAQAQELLGEQASKQTSTTRTPALD
                     EFGVDLTTLARQDKLDPVIGRESEIERVIQVLSRRKKNNPVLIGEAGVGKTAIVEGLA
                     LRIVSSNVPEILLRKRVVQLDLAGMVAGTKYRGQFEERLKAVVKEIQQANNVIMFIDE
                     LHTLVGAGAAEGAIDASNMLKPALARGELQCIGATTMDEYRKYVEKDRALERRFQAIH
                     VGPPSVDETIRILREIKDRYETHHQAIITDEAVVAAARLSQRYIADRFLPDKAIDVID
                     EAGARARLKGMTLPSELREMENEVERLRAQKEDAIRTQAFEVAARLRDTERKLRAELE
                     ERKANWKDQRAKTKTVVSEEDIAYVVAKWTGIPLQQLEEAESAKLLRMEAELGKRVVG
                     QEEAIKAVARAIRRSRAGVKNPNRPVGSFIFLGPTGVGKTELAKALAEFLFGTEDALI
                     RVDMSEYMERFSTSRLIGAPPGYIGYDDSGQLTEKVRRRPFSLVLLDEIEKAHPEVFN
                     LLLQILEDGRLTDNYGRVVDFRNTILIMTSNVGTRQFTLHSAMGFAKSDEAQTYEKMK
                     ETVTNELRRAFNPELLNRIDEVTVFHPLTLDHMRRIVDLMLGRLQDQLKEKQMSL"
BASE COUNT         1006 a         1838 c         1944 g          942 t
ORIGIN      
        1 ggggggcggg tcctgtctct ccgcgggcac ggaaaggcct cgtttgcgca tatccaggat
       61 cggaccggca agatccagat ctacgtgcgg caagacgcgg tgggggagac ggcgtaccac
      121 ctgttcaaac gtgtggatgt cggcgatttc ttgggcgtgg cggggcacct cttccggaca
      181 aagacgggcg agttgacggt ccatgtcgaa acgctcacgc ttctggcgaa agccctgcgc
      241 ccactgcccg acaagtggca cggactctcg gatgtcgagg ttcgctaccg acagcgctat
      301 gtggacctga ttgccaatcc gccggtggtg gagctgttcc ggcgacgcgc caggatcatc
      361 ggcgagattc gccgcttctt cgatgcccgg ggattcctcg aggtcgaaac acccatgatg
      421 cagccgatcc ccggcggcgc gacggcccgg ccattcatga cgcatcacaa cgccctggac
      481 ctcacgctct acctgcgcat cgcgccggag ctgcacctca agcgctgcgt ggtgggcggg
      541 ctggagcggg tgtacgagat caaccggaac ttccggaacg agggcctgga cacgcagcac
      601 aaccccgagt ttaccatgct ggagttctat caggcctacg ccgactaccg ggatctcatg
      661 gtcctgacgg aggagcttct ctccggggtg gtccaggccg tgtgcggggg cgagaccatc
      721 acgtaccagg ggcgctcgat cggcttcacg cccccctggc cgcggctgtc cgtcgaggaa
      781 ggcctagtga agctgggcgg gctccagccg gaacaggtcc tgacggcgga aggcctccgg
      841 gcggcagccg aggggcgcgg cgtgccgctc aatccggcct ggggctggtc caagctcctg
      901 gtcgagctgt ttgaggtcct ggtggagcca cagctcttcc agccaacctt tgtcaccgac
      961 ttcccctcgg agctttcgcc gctggccaag gcccggccgg acgatccccg ctacgtccag
     1021 cgcttcgagc tgtacctggg cggcctggag gtcgccaacg cctacaccga gctgaacgat
     1081 ccccgcgagc agcggcgccg gttcgaggcc caggtgaagg cccgcgccgc cggcgacctg
     1141 gagtcccacc tgatggacga ggactacctc cgagcgctgg agtacggcct gccccccacc
     1201 gccggcgagg ggatcgggat cgaccgactg gtgatgctcc tgaccgacgc cccatccatc
     1261 cgcgacgtga ttctcttccc gttgctccgt ccggaagccc gcagtgccga gccctccgcg
     1321 atcgaggcgg cgcccggcgc cccccccgag taggagaggc atggccctgt cccttcccta
     1381 cgaggtcttc atcagcctcc gctacctgaa ggccaagcgg aggcagcgct ccgtctccgt
     1441 catctcgttc atctccacgg cgggcgtggc cgtgggggtc atggcgctga tcgtggtgat
     1501 ggcggtgatg agcgggttcg agttcgacct ccgcaacaag atcctgggaa ccaacgccca
     1561 catctgggtc ctgcgctacg gcgagcgggg catcgaagac ccggtcgccg ccctgacggc
     1621 catccgccag gtgccgggcg tcacggcggc ctcgccgttc acctacaacg aggtcttgct
     1681 cacggccggg gggcggacgt cgggcgccgt gctgcggggg gtggatctgg cgtcggccgt
     1741 cgagaccacc gatttccgca agaccctcaa gagcgcgggg gccgatctca ccgcggcgat
     1801 gaatactccc cgcccggcct tccctccgga cgggatcgtc ctcggctcga tcctggccgg
     1861 cacccttgga gtgagcgtgg gaagtcctgt ggatgtcctg ctccccttgg gaggggatct
     1921 ctcgcccctg ggggtgatcc cccggatgcg ccgcttcaac gtcgtcgggg tcttccagac
     1981 ggggttcgcc gaattcgact cgaagctggc catgatcgcc atcccctcgg cccagcagct
     2041 cttccgcatg gggtcggccg tcacggggat cgaggtccgg gtgcaggaca tctaccaggc
     2101 ggcgggaatg gccgaggcca tccgcgccaa gctcaagttc ccctatttca cccgcacctg
     2161 gatggagctg aacaagaact tcttctccgc cctccgggtg gagcgaatgg tcatgttcgt
     2221 catcctgacc atgatcgttc tggtggcggc cttcgggatc gtgagcacgc tgatcatgct
     2281 ggtcatgcaa aagcggaagg agatcgccat cctgaaatcc atgggcgcga cgaatcggag
     2341 catcatgggc atcttcatcg tgcaaggggt catcatcggg attgccggca cggcgctggg
     2401 gttgcttgcc ggcctcctca tcgcgaacaa cttggacccc atcgtggccg ctatagagtg
     2461 ggtcttcggc ttcaaggcct tcccccagga cgtctacctc ctggacaagc taccctcccg
     2521 ggtgctctgg ccggacgtca tagccatagg gatcaccgcg ttcctggtga gcctcctggc
     2581 gaccatcctg ccctcgcggc aggccgccca gatcgatccg gtagtgtcgc tgcgatacga
     2641 gtgaggggaa tgggcgagcc gaaagccacg ggtcgcgata gaggattcga atgagcgacg
     2701 ggatcagggt ccaggacgtg acaaagtgct tcgcccgcga cggggaccgg gtggaggtgc
     2761 tcaagggggt cagcttcgac atcgcccccg gtgagctggt ggcgatcgtg ggcccctcag
     2821 gggtggggaa gagcaccttg ctacacctcc tgggggccct ggaacgcccc accggcgggg
     2881 agatccgtta cggggagacg gtcctgtcgg gcctctccga tgcggacctg gccgaattcc
     2941 ggaaccggcg ggtggggttc gtcttccagt ttcaccacct gctgccggag ttcaccgcgc
     3001 tggagaatgt catgctgccg cttttgatcc ggcgctgccc ggccggccag gcgaaggagc
     3061 gggcagccgt gctgctgggc ctggtgggcc tgaccgagcg gctgcggcac cggccgggag
     3121 agctgtcagg cggggagcag cagcgggtgg cgctggcccg ggcgcttgtg ggagagccga
     3181 cggtcctctt ggcggatgag ccgacgggaa acctggacag caagacggga gacgaggtct
     3241 ttgcccttct gcgccagctg aaccgcgagc ggcgtctgac cagtatcctc gtcacccaca
     3301 acgagaccat ggccaggcag gcggatcgga ttctccggat gttggacggg cgcatcgagg
     3361 gagagggtcc aacccccacc gccgaggctc cccgcgccct ccaaatttga ccctttcagt
     3421 ctgcgctttg gtccggtata atgccgtcac aaacggtgtg cgcttcccgt gcgccgccgg
     3481 agggtaacga ggcctcgagg tcggcatgag gagccagaaa tgtttgaacg attcaccgaa
     3541 cgcgcccgaa aagtcatcat ccttgcccgc gaagaggcca ttcgcctcgg ccacaatttc
     3601 gtcggcagcg aacaccttct gctaggtctg atccgcgagg gagacggcct ggccgtcgcc
     3661 atcctgaaga agctgaacgt gagcgtctcg gccctcaagg ctgagatcga gaagatcgtg
     3721 gcggtgggga cggagttctc gcccgccggt gagattccat tcactcccca ggccaagaag
     3781 gtgctggagt acgccatctc cgaagcgcgt tcgctggggc ataactacat cgggacggag
     3841 cacctgctgc tcggtctcat tcgcgagggc gaggggatcg cctccttggt cctgcgggat
     3901 ttcggcgtca gcgtggcggc tgccaaggcc caggctcagg aactgctggg cgagcaggcc
     3961 tccaagcaga cctccaccac ccggacgccg gccctggatg agttcggcgt ggatctcacc
     4021 acgctggccc gccaggacaa gctggatccg gtcatcggcc gcgagagcga gatcgagcgg
     4081 gtcatccagg tcctgagccg ccgcaagaag aacaaccccg tgctgatcgg cgaagcgggg
     4141 gtgggcaaga ccgccatcgt ggagggtctg gccctgcgca tcgtctccag taacgtcccc
     4201 gagatcctgc tgcggaagcg ggtggtgcag ttggatctgg ccgggatggt ggccgggacc
     4261 aagtaccgcg ggcagttcga ggagcggctg aaggccgtgg tgaaagagat ccaacaggcc
     4321 aacaacgtca tcatgttcat cgacgagctg cacaccctgg tgggggccgg ggccgccgag
     4381 ggggccatcg atgccagcaa catgctcaag cctgccctgg cccggggcga gcttcaatgc
     4441 atcggcgcca ccaccatgga tgagtaccgc aaatacgtgg agaaggaccg agcgctggaa
     4501 cgccgcttcc aggcgatcca cgtggggccg cccagcgtgg acgagaccat ccgcatcctg
     4561 cgggagatca aggaccggta cgagacccac catcaggcca tcatcacgga cgaggcggtg
     4621 gtggccgcgg cgcgcctctc ccagcgttac atcgccgacc ggttcctacc ggacaaggcc
     4681 attgatgtga tcgacgaggc cggggcccgg gcgcgcctca aggggatgac gctgccgtcg
     4741 gagctgcgcg agatggagaa cgaggtcgag cggctgcgcg cccagaaaga ggacgccatc
     4801 cgcacccaag ccttcgaggt ggccgctcgc ctgcgcgaca ccgagcggaa gctgcgcgcc
     4861 gagctggaag agcgcaaggc caactggaag gatcagcgag ccaagaccaa gaccgtggtg
     4921 agcgaggagg acatcgcgta cgtggtggcg aagtggaccg ggatccccct gcagcaactg
     4981 gaggaagccg agtcggcgaa gctcttgcgc atggaggcgg agctgggcaa gcgggtggtg
     5041 ggacaggagg aggccatcaa ggccgtggcc cgcgccatcc gccggtcccg ggcgggggtc
     5101 aagaacccga accgtccggt gggttccttc atcttcctgg gacccacggg ggtgggcaag
     5161 acggaactgg ccaaggccct ggcggagttc ctgttcggga cggaagacgc cctgatccgg
     5221 gtggacatgt cggagtacat ggagcgcttc tccacctccc ggctcatcgg ggcgcctccg
     5281 gggtacatcg gctacgatga ctcgggccag ctcaccgaga aggtgcggcg gcgtcccttc
     5341 tccctggtcc tcctggatga gatcgagaaa gcccaccccg aggtcttcaa cctgctgctg
     5401 cagatcctgg aggacgggag actcaccgac aactacggcc gggtggtgga cttccggaac
     5461 acgatcctga tcatgacctc caacgtcggc acgcgtcagt tcactctcca ttccgccatg
     5521 gggttcgcca agtcggatga ggcgcaaacc tacgagaaga tgaaggagac ggtgaccaac
     5581 gagttgcgac gcgctttcaa ccccgagctg ctgaaccgca tcgacgaggt gaccgtcttc
     5641 cacccgctca ccctggacca catgcggcgg atcgtggacc tgatgctggg ccggctgcaa
     5701 gaccagctca aggaaaagca gatgagcctc
//
LOCUS       DBNGZC010000380         5149 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2672092, whole genome shotgun sequence.
ACCESSION   DBNGZC010000380 DBNGZC010000000
VERSION     DBNGZC010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5149)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2672092"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="AC16G0_06730"
     CDS             <1..1109
                     /locus_tag="AC16G0_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008539412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5099355.1"
                     /translation="KALIQDLADFQIPALLFSGGEPLARPDLFELVSHARSRGIRPTL
                     STNGTLITDETARKIKDAGFTYVGISLDGIGEINDKFRGVGGAFDRAVRGFKSCVQVG
                     QRVGLRLTLTRHNFTNLHQIFDFIEREQIDRACFYHLVYSGRGEQIAADDLSREETRQ
                     AIDIILQRTEDFHRRGLNKEILTVDNHVDGIYLYLKLREKDPSRAEQVLKLLRWKGGG
                     MYSSGVGIADIDFLGNVHADQFWMHHSFGSVRQRPFGQIWMDTSDPLMAGLKNRRTKI
                     KGRCSPTVCKWFDACGGAMRVRADLVHNDPWAPEPACYLTDEEIGFTDEKKAAVKARG
                     EDFPMPAFSRPPADAQSQSFRNPPTTACTGGSEA"
     gene            complement(1180..2184)
                     /locus_tag="AC16G0_06735"
     CDS             complement(1180..2184)
                     /locus_tag="AC16G0_06735"
                     /inference="COORDINATES: protein motif:HMM:NF020650.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099356.1"
                     /translation="MRKVQGIVWVIILCAVWAGPCTAGERVRLAISATALSMLPVYLA
                     QGLALFSDEGLDVEGIVTQGAGLEIKALAAEQVDFAFTSGDAILAAIQQGQPLAIVFS
                     GMNRPIVNWAMHKDVAMQRGLAASSPLDRKLRELKGLTIGLDQRGGLAEHLADYALRK
                     VGLKPNSDARIVSLGSGPEWLTRLRSRRADGALGMVPLPEMAVAQGSAVLFIDNARGE
                     DPSFPEFLMANLVARRDYLEKNPGIARKMVRALYRANRWALANPPERMADMLQPFLSR
                     IEPSALLEGVKLVLPAVTPHGRTTQRAVGVTFDILEEAGLLKKRAPFSEIVVNDYVPG
                     "
     gene            complement(2807..4855)
                     /locus_tag="AC16G0_06740"
     CDS             complement(2807..4855)
                     /locus_tag="AC16G0_06740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR5099357.1"
                     /translation="MRQLPHKGAGLRQRGVASLFAFVAALWLAWAWIGWQWPLPDGRA
                     GSVPTVRLYAGAGPEEIAVLEGTTPRAQVWVPLTQIPRSVVGAVLTTEDRRFFHHSGV
                     DLRAVVRAARSDLRHGEVRQGGSTITQQLARSLFLTPDRTWARKLREAAIAVALELRY
                     PKDRILEAYLNSVYIGQDGNVALYGLGTAARHFLHKELTAVRLDEAALLASAISGPNR
                     TFSGDVVRARAGRDAVLQAMREQGLVSEVVAHEAMARPLPKPSDGSFVRAPYFVDLAR
                     EEITRRASLPSSGDVRIATTLDPVLQRAAESAIRVGIQRIERRRPDLAQARPQAALVA
                     IEPASGQIRALVGGRRYLDSPLNRATRAARQPGSLFKPIVYLAAFEAERTGRLPGLTP
                     ASLIPDEPLAIQAAGETWSPRNLDRRFHGPVTVRRALEESLNIPAARMAQDVGLNQVV
                     EMARALGIESPLAPVPSLALGTSEVTLLEITAAFAALANQGVRVAPTTLAPTQDTSVA
                     GLLAPTLPPVRAASPESSYLITHILQGVMSRGTAHASTAWGLSEIAAGKTGTSDGLRD
                     AWFVGYTPDLAVGVWVGLDDDSPLGLTGAQAALPIWATVMQTAVRRVPPRRFEPPPGI
                     VLAAVDLDTGRPASFWCGGTTVIQEAFRAGSEPHGGCGELPLVGKVGGFIGWLRGLFQ
                     "
BASE COUNT          906 a         1760 c         1656 g          827 t
ORIGIN      
        1 gaaaagccct gatccaggac ctggccgatt tccagatccc tgcgctgctc ttctccggcg
       61 gcgagccgct ggcgcggccc gacctcttcg agttggtctc ccacgcacgt tcccggggga
      121 tccggcccac cctgtccacc aacggcaccc tcatcaccga tgaaacggcc cggaagatca
      181 aggatgccgg cttcacctac gtcgggatca gcctggacgg catcggggag atcaacgata
      241 aattccgggg ggtgggtggc gccttcgacc gggccgtgcg gggattcaag tcctgtgtcc
      301 aggtcggcca gcgggtgggc ctgcgcctga ccctcacgcg acacaacttc accaaccttc
      361 accaaatctt cgacttcatc gagcgggagc agatcgatcg cgcctgcttc taccacctcg
      421 tgtattccgg gcggggtgaa cagatcgcgg ccgacgacct ctcgcgggag gagacccggc
      481 aggccatcga catcatcctg cagcggaccg aagatttcca ccggcggggg ctcaacaagg
      541 agatcctgac cgtggacaat cacgtggacg ggatctacct ctacctcaag ctgcgggaga
      601 aggatccgtc ccgggcggag caggtcttga agctcctgcg ctggaagggg ggtgggatgt
      661 acagttctgg cgtcggcatc gccgatattg atttcctggg gaatgtccac gcggatcagt
      721 tctggatgca ccattccttc gggagcgtgc gacagcgccc gttcggtcag atctggatgg
      781 acacctcgga ccccttgatg gccgggctga agaaccggcg gaccaagatc aagggccgct
      841 gctcgcccac ggtctgcaag tggttcgatg cctgtggcgg ggccatgcgg gtgcgggcgg
      901 acctcgtcca caacgatcca tgggcgcccg agcccgcgtg ctacctgacc gacgaagaga
      961 tcggcttcac cgacgagaag aaggcggcgg tcaaggccag aggcgaggac tttcccatgc
     1021 ctgccttttc ccgtccgcca gcggacgccc agtcccagtc gtttcgcaat ccgcccacca
     1081 ccgcctgcac cggcgggtcc gaggcctgaa gacccggcca cccagccctc gctgcggagc
     1141 ggtcggcctc gacccggcgg ttggacgcta atcttcctcc tatccgggaa cgtagtcgtt
     1201 gaccacgatc tccgagaagg gggctctctt tttcaacagg ccggcctcct ccaagatatc
     1261 gaaggtaacc ccgaccgccc gctgcgtcgt cctgccatgc ggggtgacgg caggcaggac
     1321 gagcttcacg ccctccagaa gcgcggaggg ttcgatccgc gaaagaaagg gctggagcat
     1381 gtcggccatc ctctccggtg ggttcgccag ggcccagcga ttggcccggt acaacgcccg
     1441 caccatcttc cgagctatcc ccggattctt ctccaggtag tcgcggcggg caacgaggtt
     1501 cgccatcaga aattcgggga atgacggatc ttcccccctg gcgttgtcga tgaacaacac
     1561 ggcgcttccc tgggctaccg ccatttccgg aaggggtacc atgccgagcg ccccatcagc
     1621 ccgccggctt cgcagcctgg tcagccactc gggtccgctt cccagagaca cgatcctcgc
     1681 atccgagttg ggcttgaggc ccaccttccg caaggcatag tccgcgaggt gttcggcgag
     1741 gccaccgcgc tggtccaagc cgatggtcag acccttgagc tcccggagct tgcgatccag
     1801 gggactgctc gcggcgagcc cgcgctgcat ggccacatcc ttgtgcatgg cccaattcac
     1861 gattggacgg ttcatgcccg agaacacgat agccagtggc tgtccttgct ggatcgcggc
     1921 caagatagcg tcgccgctgg tgaaggcaaa atccacttgc tcggccgcca gcgccttgat
     1981 ctccaacccc gctccttgcg tgacgattcc ctcgacgtcc agaccctcgt cagaaaagag
     2041 ggccagtccc tgagccaggt acacgggcag catggagagc gcggtggcgg agatggcgag
     2101 cctgaccctc tcgccggcgg tgcaggggcc ggcccagacg gcacacagga tgatcaccca
     2161 gactattccc tgaaccttgc gcactacatc cttcctctcg gcggtcggaa tacctcacat
     2221 cctgaggttc gtcttcgcga gcagcgcccg gcaccggcag tcttccagga cggcgtccac
     2281 acccgcattc actcgatccc gactgagatg aaacggttcg aaagtcgtga cgccagcctt
     2341 cctacgtcgg acgcggcgcc gatggtggag ggcggcgcaa cggccactcg ataacctgcg
     2401 tcgatctcac cggcagctcc ccagagcctc tccccgtgcg ggtgatcgga attctagcag
     2461 cgatcgggtc atcccagcaa ccagcatccg ttccttccag acccaagcag ggattagtcc
     2521 tcacgaattg gtgcactccc tggaagcatg gagtccgagc ggtctcacgc acccgcagga
     2581 accctggatg aaggtggtat ggggattccc ggtggaaggc tgatcggtgg gacgtcgcgc
     2641 gcacgctgcc ggcctcctca ggccacgggc gaccttcaga acgtggtgag tcctcggatg
     2701 cggtagcgac ctctctggga ggtagcccct cccggcggga actccggcgg gcgtaacacc
     2761 cgcgctatgc tcaaggggtg aacggtagga cgagagagaa tcgtggctat tgaaacagcc
     2821 cgcgcagcca gccgatgaag cccccgacct tccccaccaa gggaagttcg ccacaaccac
     2881 cgtggggctc gcttcccgcc cggaaggctt cctggatgac ggtggtgccg ccacaccaga
     2941 atgaggccgg gcgacctgta tcgaggtcaa cggcggcgag aacgatgccc ggcgggggct
     3001 cgaagcggcg ggggggaacg cgccggacgg ccgtctgcat cacggtcgcc cagatcggga
     3061 gcgccgcctg cgccccggtg aggcccagcg ggctgtcatc atccagaccc acccacaccc
     3121 ccaccgccag atcgggcgtg tacccgacga accaagcgtc ccggaggcca tccgaggtcc
     3181 cggtttttcc cgctgcgatt tcgctcagcc cccaggccgt gctggcgtgg gcggttcccc
     3241 gactcatcac cccctgcaag atgtgggtga tgaggtagct cgactcgggc gaggcggcac
     3301 gcacgggggg caaagtcggg gcgaggagcc ccgccacgga cgtgtcctgc gttggggcaa
     3361 gggtcgtcgg tgcaactcgg accccctgat tggccaaggc ggcgaaggcg gccgtgatct
     3421 ccagaagcgt cacctccgac gtgcccaagg ccagagaggg gacaggggct aacgggctct
     3481 cgatgcccaa agctcgcgcc atctccacca cctggttcag tccgacatcc tgcgccatgc
     3541 gggcagcagg gatattcaga gattcctcca gcgcccggcg gaccgtcacc ggcccgtgga
     3601 agcgacgatc gagattgcgc ggcgaccacg tctcccccgc cgcctggatc gccagcggct
     3661 cgtccgggat gagggacgcg ggcgtgaggc cgggcagcct tccggtccgt tcggcctcga
     3721 aggcggcgag atacacgatg ggcttgaaga gtgatcccgg ttggcgggct gcccgcgtgg
     3781 cccggttaag gggactgtcg aggtagcgcc gcccgccgac cagcgcccgg atctgcccgg
     3841 aggccggctc gatggcgacc agcgcggctt gaggcctggc ctgtgccagg tcaggtcgcc
     3901 gtcgttcgat acgctggatg ccaacccgga tggccgattc ggcggcgcgc tggaggaccg
     3961 gatcgagggt cgtggcgatc cggacgtcac ccgacgaagg gaggctcgcg cgccgggtga
     4021 tctcctcgcg agccaggtcg acgaagtagg gcgcacggac gaaggatccg tcggacggct
     4081 tcgggagcgg acgggccatg gcctcgtgcg ccaccacctc gcttaccagc ccctgttcgc
     4141 gcatggcctg caaaacggca tcccgtccgg ctcgcgcccg cacaacatca cccgagaagg
     4201 tccggttggg cccgctgata gccgaggcca gtagcgcggc ttcgtccagg cggacggcgg
     4261 tcagctcctt gtgcagaaaa tgacgcgccg cggtgcccaa accatagagc gcgacgttgc
     4321 cgtcctgccc aatatagacg ctgttcaggt aggcttccag gatacggtcc ttggggtatc
     4381 gcagctccag cgccacggcg atcgccgcct cgcgaagctt gcgggcccag gtccgatctg
     4441 gcgtcaggaa gaggcttcgg gcgagttgtt gggtgatcgt actcccaccc tgtcgcacct
     4501 cgccatgccg caggtccgat ctggccgcgc gcacgacggc ccgaaggtcg acgccgctat
     4561 gatggaagaa gcggcgatcc tcagtcgtca ggaccgcccc taccaccgat cgggggatct
     4621 gagtcaaggg cacccagacc tgcgcccggg gcgttgttcc ctccaggacg gcgatctctt
     4681 cgggcccggc tccggcgtac agccggaccg tcggaacgga accggcacgc ccatcgggga
     4741 ggggccactg ccagccaatc caggcccagg caagccacag cgcggccacg aaggcgaaca
     4801 ggctggcaac gccccgctga cgaaggcccg cccccttgtg gggaagttgg cgcaaaccag
     4861 cagtttcagg atttgggtgc agccttcccg cgctcaccag cactcctaga aagtctgcct
     4921 catcacctgg ccggagaccc cactcctctg catctgcgat gcggcatgcc ccgatgtggc
     4981 atagcgcatc aggctgtcat gctacttgtt gtgcaaaccg taaagggtgc ctgcggtcgc
     5041 gccgacaatc ccaccgatcg cggccccctt gccggccccc ttgccgcctc cggcgatggc
     5101 gccgcctgcc gcccccacac cggcacccag ggcgcccccg atcaccgcc
//
LOCUS       DBNGZC010000381         1092 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2282320, whole genome shotgun sequence.
ACCESSION   DBNGZC010000381 DBNGZC010000000
VERSION     DBNGZC010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2282320"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="AC16G0_06745"
     CDS             <1..1049
                     /locus_tag="AC16G0_06745"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5099358.1"
                     /translation="RFGPRRVVLAGGLLLPLAMAANAAAHHQWHLYVTHGVLAALALS
                     ATGYVPVSLVLTHRFQKDRGLALGTASAGVGVGILVLVPMTQVFIDSCGWRIAYIALA
                     AISAAVVLPVGFFALGEERIASPSEGEQPAAGTPHSSQDSRAARDWTLASALRSRTFW
                     LVTATFVLLNGPTQLVLTHHVAYLVELGHPKFLVVGIVGLVGLFSIPGKIGWGLLSDR
                     WWPELIYVAGGACAVAAILTLLIIDPDSSIWSLYAYAVLMGFGYAVSPAMTPILSGRF
                     FVGRHFGVILGALNTFYQSAGAAGIWLAGYAHDMTGSYRLPLLGSIVSASLAVACVWL
                     AAPRRFPASNRRAR"
BASE COUNT          131 a          395 c          327 g          239 t
ORIGIN      
        1 atcgctttgg accccgtcgc gtggttctgg ccggcgggct gctcttgccc ctcgccatgg
       61 cagccaatgc tgccgcacat caccagtggc acctgtatgt cacccacggc gtcctggctg
      121 ccctggccct ttccgccacg ggctatgtgc ccgtctccct tgtcttgacc caccggttcc
      181 agaaggatcg cggcctggcg ctcggcaccg cctcggccgg cgtcggcgtc gggattctgg
      241 tcctcgtgcc gatgacccag gtgttcatcg actcctgtgg ttggcgcatc gcgtatatcg
      301 cacttgctgc catctccgcg gcggtggtcc ttccggttgg attcttcgct ctgggcgagg
      361 agagaatcgc ctcgccctcc gagggggagc agccggctgc cgggactccc cacagctccc
      421 aggactcccg cgcggcgcga gactggaccc tggcctcagc cctccgcagc cgcacgttct
      481 ggctggttac tgccaccttc gtcctcttga acggcccgac ccagttggtg ctgacccatc
      541 atgtggccta cctcgtggag ctgggtcatc ccaagttcct ggtggtggga atcgtggggc
      601 tggtgggcct gttcagcatc cccgggaaga tcggatgggg gctcctgtca gaccgatggt
      661 ggcccgagct gatctacgtg gcaggcggcg cctgcgcggt cgccgcgatc cttactcttc
      721 tgatcatcga tccggactct tccatctgga gcctgtatgc gtacgccgtc ctcatgggct
      781 tcggctacgc cgtctcgccc gccatgaccc cgatcctttc aggccgcttc tttgtgggcc
      841 gccatttcgg cgtcatcctt ggcgccctga acaccttcta ccaatcggcc ggtgcggcgg
      901 gtatatggct ggccgggtac gcccacgaca tgacaggaag ctatcggctg cccctcctcg
      961 gctccatcgt cagtgccagt ctggctgttg cctgcgtctg gctcgccgct cctcgccgtt
     1021 tccctgcgtc caaccgacgc gcccgctgag atacctgcgg ccattcattc ccgccgccgt
     1081 ccgcgaacgc tc
//
LOCUS       DBNGZC010000382         1368 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_422730, whole genome shotgun sequence.
ACCESSION   DBNGZC010000382 DBNGZC010000000
VERSION     DBNGZC010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_422730"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="AC16G0_06750"
     CDS             complement(<1..1102)
                     /locus_tag="AC16G0_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099359.1"
                     /translation="MATTARARTKPTGRTMGVLILALAVVLALTMAVRTDAQTPVRGG
                     TLRVGWIPDAKTLDPHFSVQFAERHILYMIFNTLVGLDKSFNVIPELARSWQVSQDGK
                     RVTFQLQRGVKFHDGTDFTAEVVKWNIERILDPATKSPQRSQLEPAVAAVTVLDPYTV
                     AFEMKKPFAPLLAALAERPGFIVSPAAVKKWGENFGQHPVGTGPFRFVEFVQDSRVVL
                     ERFDGYWEKGKPYVDKVVCQVVPDPTVRSTMVRTGEVDIATDVEVKDLPPLQADPTLR
                     VSEMKPPARWTVLQWQVDKPPFNNKALREAIALGINREELKQVLLQGYGEVARGPVTP
                     GLWWQDQNLKGYGYDPELAKKKLAEAGYPNGFH"
     gene            complement(1177..>1368)
                     /locus_tag="AC16G0_06755"
     CDS             complement(1177..>1368)
                     /locus_tag="AC16G0_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099360.1"
                     /translation="GDILFVPGWNGLRFVYGPAQMRGPAGTLPVPRIGRLAGDLTDFV
                     KLAKRIEWEGAKKMAIARG"
BASE COUNT          234 a          470 c          410 g          254 t
ORIGIN      
        1 agtggaagcc gttcggatac cccgcctcgg cgagcttctt ctttgccagc tcggggtcat
       61 aaccgtagcc tttgagattt tggtcctgcc accacaggcc tggggtcacc ggcccgcgag
      121 ccacctcgcc gtagccctgc aggagaacct gcttgagttc ctcccggttg atccccagcg
      181 cgatcgcctc gcggagggcc ttgttattga atgggggctt gtccacctgc cactggagga
      241 cagtccagcg ggccggcggc ttcatctcgg agacccggag ggtcggatcg gcctggagcg
      301 ggggcaagtc cttgacctcc acatcggtgg cgatgtccac ctcgcccgtc cggaccatgg
      361 tgctccgaac cgtcggatcc ggcacaacct gacagacgac cttgtcgacg tacggctttc
      421 ccttctccca gtacccgtcg aagcgctcga ggacgactcg agaatcctgc acaaactcca
      481 cgaagcgaaa ggggccggtc cccacggggt gctgtccgaa gttctctccc cacttcttca
      541 ccgcggccgg agacactatg aaacccggcc gctccgccaa ggccgccagg agcggcgcaa
      601 agggcttctt catctcgaag gcgacggtgt aaggatccag gacggtcacg gccgctacgg
      661 caggctctag ctgacttcgc tggggggact tcgtggcagg atccaggatc cgctcgatgt
      721 tccacttgac gacctctgcc gtgaaatccg tcccgtcgtg gaatttcacc ccccgctgca
      781 actggaacgt cacgcgcttg ccgtcctgcg atacctgcca ggagcgggcc agttccggga
      841 tgacgttgaa gctcttgtcc agaccgacaa gggtgttgaa gatcatgtac aggatgtggc
      901 gttccgcgaa ctgcaccgag aagtgagggt ccagggtctt ggcgtcggga atccacccta
      961 cccggagcgt gccgccacgg acgggcgtct gcgcgtctgt ccgcacagcc atggtcagcg
     1021 ccaggaccac agccagcgcc aggatgagca cccccatcgt tctgcctgtt ggtttcgtgc
     1081 gtgccctcgc cgtagtagcc atcgccagcc ccctcctttg cgagcgcgtc gatatccgag
     1141 accctggcgt gggtctccct cgccttgcca tacggctcac ccccgggcga tcgccatctt
     1201 cttggcgccc tcccattcaa tccgcttcgc cagcttcacg aagtctgtca ggtcgccagc
     1261 gaggcggccg atccgcggca ccggaagggt ccctgcaggc ccgcgcatct gggccggtcc
     1321 gtacacaaag cgcaggccat tccagccagg aacgaacagg atgtcacc
//
LOCUS       DBNGZC010000383         2055 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1205777, whole genome shotgun sequence.
ACCESSION   DBNGZC010000383 DBNGZC010000000
VERSION     DBNGZC010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1205777"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="AC16G0_06760"
     CDS             complement(<1..180)
                     /locus_tag="AC16G0_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099361.1"
                     /translation="MRKGDRSNPGLVSEPFPASLAAVSATPATAHWLTWLCLSRLSLS
                     LIFNAYAGVLPLVRQA"
     gene            complement(218..1372)
                     /gene="argH"
                     /locus_tag="AC16G0_06765"
                     /pseudo
     CDS             complement(218..1372)
                     /gene="argH"
                     /locus_tag="AC16G0_06765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
     gene            complement(1385..1753)
                     /locus_tag="AC16G0_06770"
     CDS             complement(1385..1753)
                     /locus_tag="AC16G0_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099362.1"
                     /translation="MPGHRKKLTAARPTRRAMRPESRFSEPPAPEMQAYVSTRRAAET
                     RYGFTQELWNHRAHTIMLCEEEILRRKDAARILRALDEIGRIGPDRFPIDPGTGELLF
                     SVERYLQRRIGDDTASRLHT"
     gene            complement(1729..>2055)
                     /locus_tag="AC16G0_06775"
     CDS             complement(1729..>2055)
                     /locus_tag="AC16G0_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099363.1"
                     /translation="FSETRLQDPRIRTWMQKVEVRSVPELPVRARLTAHRQDGRTATI
                     EPRLRSLEPGEVRSKFQEVSTPLLGAKVTEAILRAVDNLEQAEDPHTLSRLLRRSVRC
                     QDIARS"
BASE COUNT          342 a          654 c          682 g          377 t
ORIGIN      
        1 ggcctgccgg accaagggaa ggacgccggc ataggcgttg aagatcagcg agagcgacag
       61 acggctgagg cacagccacg tcagccagtg ggccgtcgcc ggcgtggcgg agacagccgc
      121 cagggaagcc gggaacggct cgctcaccag tccgggattg ctccgatctc cctttcgcat
      181 ctgcgcagcc cattagccta cagtctggca gccggcctca tttgatgatc tgtttggccc
      241 gctggtcggt catccttgcc cccctctgga ttgattcccg acaggatcga atccatttca
      301 tctcgcgcgc gagggatccg gcgcgctgct gcagcatccg tccaacctga cgcagtcccg
      361 ggccgcccgg cgttttcctc ctccgaatgg tgagcgccac gtccatacat tggcggaggg
      421 ttgcggggcg catttccagc ggccgaccca ggatcgcgat ggccgcctgg ttgatcatgg
      481 cgggtttcac ctgcgctggt gtcatctgcg tctggatgca gtctcggaca agccggccca
      541 cgatacgatg agccactcga aacgggaggc cttcttcctg cgcgatcgtc tctgccagct
      601 gcggagcctg gatgaagttg gccccggcga gctgcgccat ccgctcaggc ttccccttca
      661 acgtcgccgt caccgcccgg aggagatcgg tggcggcaat cgcggtgtcc agggtgcgcc
      721 agaccaaggg gccggttgcc tccagatcat tcaactgctc cgagtgggtc ttgagctgca
      781 tgaaggccgt cgtggtgttg cccacgcact ccgctgccag atgccggacc cgcgtcaacg
      841 cgctcggatt cttcttctgg ggcatgatgg aggaggttcc agaaaaagcg tccgccagct
      901 cgatcagccc gaactcgtag gtgcaccaga gtatcagatc ctcgcaaaac gtcatgaggt
      961 tcgccgccag gatagcggtg tgggtgagcg tttcgaggct gtggtcccgc gcggtggaag
     1021 catcccgcgt attctggatg accccgtcaa atccgagcaa ggccgccatc cgcggacggt
     1081 tcaccgggaa gccgctcccc gacagaatgg cggagcccat ggggctccga ttgacgcgac
     1141 ggtaggtctc ccggaggcgt gtgaggtcgc ggaagaaacg atcggcgaag gagagcaggt
     1201 agtggccgaa ggtgatgggc tgcgcgtgct gcaagagcgt gtagcccggc atgaccgtgg
     1261 cggtgtgctg agcagcttgg gccagcaagc tctgtatgag gcggaccagg acgtcggcaa
     1321 gcgccaagag cttctcccgg aacaccatcc gctcgacaca gacgtagagg tccccccgac
     1381 tgcgtcaggt gtgcaaccgg ctggccgtgt cgtcgccgat gcgtcgctgc aggtaccgct
     1441 cgacgctgaa cagcagctca cccgtgccgg gatcgatggg gaaccggtcg gggccaatcc
     1501 ggcctatttc gtctagcgcc ctcaggattc tcgcagcgtc cttgcgccgc aggatctcct
     1561 cctcacagag catgatggtg tgggcccggt gattccagag ttcctgggtg aagccgtagc
     1621 gtgtctcggc tgcccgccgg gtcgatacgt acgcctgcat ctccggggcg ggcggctccg
     1681 agaagcggga ttccgggcgc atggcccggc gggtcggtcg tgcggcggtc agcttcttgc
     1741 gatgtcctgg catctcacac tccggcgcag caagcgactc aaggtgtggg ggtcttcagc
     1801 ctgctccagg ttgtccacag cgcgcagaat cgcctcggtg acttttgccc ccagcagtgg
     1861 agtcgagacc tcctgaaact tcgaccgaac ctcgcccggc tcgagggagc ggagccgcgg
     1921 ctcgatcgtg gccgtcctcc catcttggcg atgggccgta aggcgtgccc ggaccggcaa
     1981 ttcgggcacg ctccggacct cgaccttttg catccaggtc cgtatccgcg ggtcctggag
     2041 cctggtctcg gaaaa
//
LOCUS       DBNGZC010000384         8081 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2672467, whole genome shotgun sequence.
ACCESSION   DBNGZC010000384 DBNGZC010000000
VERSION     DBNGZC010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 8081)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8081)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8081
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2672467"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="AC16G0_06780"
     CDS             <1..592
                     /locus_tag="AC16G0_06780"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140664 - ATP-dependent DNA damage sensor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadA family protein"
                     /protein_id="HIR5099364.1"
                     /translation="MRAEGLAPVENPSALFLAERPAHATGCVVVATLEGTRPILVELQ
                     ALVSPTGAAIPRRVANGLDYQRVAMLLAVLEKRLKIGLGASDVFLNVVGGLGVREPAA
                     DLAVLAAVISSVRELPVEPAWAFFGEVGLGGEIRAVPQTERRLLELQRLGFSRAVTAR
                     AAVEKLATPSVIEAVGLAHVGELADLLLPAGKIRFD"
     gene            648..1130
                     /locus_tag="AC16G0_06785"
     CDS             648..1130
                     /locus_tag="AC16G0_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009303 - rRNA transcription [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HIR5099365.1"
                     /translation="MFQVGQKVVYPTHGVGRIEAIEQKEVSGSRQSYYILRILGNDMT
                     IMVPTGNAERVGLRQLIGRKEVPKVMEILRKKDMEICPNWNRRFKDNLERIKTGSLYE
                     VATVLRKLVLLQKERNLSFGEKKMLENVRQLLVSEISHAVGIDEGNARSMVDRTIAKA
                     "
     gene            1162..2175
                     /locus_tag="AC16G0_06790"
     CDS             1162..2175
                     /locus_tag="AC16G0_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HIR5099366.1"
                     /translation="MSESLVRVLFVFLCGAVGLLGPALQGGGGQAAIAGSSTGLVFGL
                     LVVLLERRLRHVPGKTILQALTGFVVGLLVGRVAYAAVGNVLAAFPGFVGLFAESLCL
                     VGGGYLGVLIALDKGREWNLAGFIRVLKEQPRAESYKLLDTSVIIDGRIADITETGFL
                     EGTLVIPQFILRELQHIADSSDPLKRNRGRRGLDILQKIQKKVDIRVEISDMDFPEIR
                     EVDNKLVAMAKALHAKIVTNDFNLNKVAELHGVGVLNINELTNALRPVVLPGEDMRVY
                     VLKEGKEYNQGIAYLDDGTMVVVDNGRRHIGQTIDVCVTSVLQTTAGRMIFSRLKEEA
                     EAA"
     gene            2295..2993
                     /gene="ispD"
                     /locus_tag="AC16G0_06795"
     CDS             2295..2993
                     /gene="ispD"
                     /locus_tag="AC16G0_06795"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HIR5099367.1"
                     /translation="MHVTAIVPAAGGGTRLAAPHPKQYLPLAGVPMLTRTLQALRLSR
                     RVDCVILVVPPGQEIRCRREILEPFGLEVEPLVPGGADRQASVYAGLLQAPTATDLIL
                     VHDGARPLITTALIQAAVAAAAEFGAAVVAVPVSDTIKRVAPDGRVLETPPRGQLWAA
                     QTPQVFRASLLREAHEAALRDGFRGTDDSALVERLGQPAHIVPGSPENFKITTTADLV
                     LADQILRARAAQTG"
     gene            2997..3551
                     /gene="ispF"
                     /locus_tag="AC16G0_06800"
     CDS             2997..3551
                     /gene="ispF"
                     /locus_tag="AC16G0_06800"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIR5099368.1"
                     /translation="MRQTGDLPLRVGVGFDMHRFVTGRPLILGGVQIPFERGLEGDSD
                     ADALTHAILDALLGAAGAGDIGSHFGVGRPEMQGISSLLLLERAAAIVAAKGFRPGNV
                     DATVVAEAPRLGPYIPSMRKALAEVLGLAEDRVNLKGTTAKGMGVLGAGEGIAAIAVA
                     SLIPDRVGRPGVPSHRRKGIPHPR"
     gene            3615..5198
                     /gene="cysS"
                     /locus_tag="AC16G0_06805"
     CDS             3615..5198
                     /gene="cysS"
                     /locus_tag="AC16G0_06805"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR5099369.1"
                     /translation="MTLRVYNTLTRRLETFEPLVAGQVRMYTCGITAYDLCHIGHARS
                     ALVFDGIRRYLEYAGYRVTLVRNFTDVDDKIINRARESNIPWDVVARENIAAFHRDMG
                     ALGLRPADVEPKATDHIPEMLRIIQVLLERGLAYVVDGDVYYAVRRFPEYGKLSHRSL
                     DDLMAGARIEVDERKRDPPDFSLWKASRPGEPAWDSPWGPGRPGWHIECSAMAAKYLG
                     DSFDIHAGGEDLIFPHHENEIAQSEGATGKPFARYWLHNGFVTVNGEKMAKSLGNFVT
                     IREALERFNPDALKLLLLSTNYRGPLDYTEAAVAEKTKALEGLQEFLRAVDRLTPPGS
                     SQAQIEEGSGGALHPAEAEFRAAMDDDFNTAKATGVLFDLVREGNRLLHEADQDASPR
                     PVSLAALHRTAVLLRRLGGVLGLDLQGGRSVTSKLSAVPLTRPAAEALTELGQLLQAE
                     GAGPADLEARLTAVVLELLAHREAARKARAWGEADQIREALSAHGLRIEDTRTATHAI
                     SDFPMERRRPPIDVTAVKA"
     gene            5195..5983
                     /gene="rlmB"
                     /locus_tag="AC16G0_06810"
     CDS             5195..5983
                     /gene="rlmB"
                     /locus_tag="AC16G0_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR5099370.1"
                     /translation="MKTLPHRLPGPDDAEILAGPHAILEALRAGRRRLRRILLARQQV
                     AGIIESVLELARARALPVEVRPRAELDRLVPGAAHQGIVADAEPFPYVTPEEILSQAR
                     RGDEPGFLVVLDGIQDPQNLGGIIRTVDAAGAHGLILPRDRAASVTPAAVRASAGASE
                     HVSVARVTNLATFLEWVKGQGVWVLGADSNTGRDLYSVDLTTPLALVVGSEDRGLRPL
                     VKRRCDLLVRIPVRGRVASMNASAAAAICLFEVVRQRKAVEKRL"
     gene            6121..6196
                     /locus_tag="AC16G0_06815"
     tRNA            6121..6196
                     /locus_tag="AC16G0_06815"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6154..6156,aa:Thr,seq:tgt)
     gene            6262..6347
                     /locus_tag="AC16G0_06820"
     tRNA            6262..6347
                     /locus_tag="AC16G0_06820"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6296..6298,aa:Tyr,seq:gta)
     gene            6477..6553
                     /locus_tag="AC16G0_06825"
     tRNA            6477..6553
                     /locus_tag="AC16G0_06825"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6511..6513,aa:Gly,seq:tcc)
     gene            6612..7820
                     /locus_tag="AC16G0_06830"
     CDS             6612..7820
                     /locus_tag="AC16G0_06830"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5099371.1"
                     /translation="MPSLGPERSGFWLVMTLLAGLCGLAMPAWGTNAQAQRLNEAAAA
                     RTAQGRYEEAAELFSQALRLSPDDPVIRKNLARLRTVMGHRLLRAGLLQRAQEQYQAA
                     LDLDPNEAAALLGLGDVQLWQREPRAAAESYRRVIALDPQSADAYVRLGEAYNHLGDM
                     AAAVSEWERALALRPNDAGLRQRLDQLRREEQSLGGYRSRGSQHFRVTYAGERREDLG
                     RELLQILERAYTDVGYELGVYPSQEVQTIFHSADLVEGSYHLLDGKIRVALRGPAPGD
                     PMLRAILYHEYTHALIYAATRANNPPLWVHEGLAVQMEKSGRALTYRDEAFRQARAGT
                     PPSPETSPYVYGSVAIGYLIDRYGMPGIGRLLHGMGEGLPFRTAFQEAFGVDLTTFQQ
                     GFRDLLVRGY"
     gene            7956..>8081
                     /locus_tag="AC16G0_06835"
     CDS             7956..>8081
                     /locus_tag="AC16G0_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR5099372.1"
                     /translation="MAKAKFERTKEHMNIGTIGHIDHGKTTLTAAITKALGLKNPK"
BASE COUNT         1366 a         2465 c         2867 g         1383 t
ORIGIN      
        1 gatgcgggcc gaaggcctgg ctcccgtcga gaacccttcg gcgctgtttc tggccgagcg
       61 accggctcac gccacggggt gcgtggtggt ggcgaccctg gaggggacgc ggcccatcct
      121 ggtggagctg caggccctgg tctcacccac gggcgcggcg atcccccggc gggtggcgaa
      181 cggcctcgac taccagcgag tggcgatgct cctcgccgtc ctggagaagc gtctcaagat
      241 cggtttgggg gcctcggacg tcttcctcaa cgtggtcggc ggtctggggg tgcgggagcc
      301 ggcagccgac ctggcggtcc tggcggccgt catctccagc gtccgggagc ttcccgtgga
      361 acccgcctgg gcgttctttg gcgaggtggg gctgggcggt gagatccgag ccgtgcccca
      421 gaccgagcgg cgactcctcg agcttcagcg tctcgggttc tcccgcgccg tgacggcccg
      481 agcggcagtg gagaagctcg ccacccccag cgtgatcgag gcggtgggac tggctcacgt
      541 gggtgaattg gcggatctgc tgctcccggc cggcaagatc cggtttgact gatgtggcgg
      601 agtgtggttt aattggcaga aattttgcct cacaaagggg tgcagccatg ttccaggtcg
      661 gccagaaggt cgtgtatcca acgcatggcg tgggtcgcat cgaggcaatc gagcaaaagg
      721 aggtttccgg cagtcggcag tcatactaca tcctgcggat cctggggaac gacatgacca
      781 tcatggtgcc tacggggaac gccgagcgcg tgggtttgcg ccaactcatc ggccggaagg
      841 aagttcccaa ggtcatggag atcctccgca agaaggatat ggagatctgt cccaactgga
      901 atcgccgttt caaggacaac ctggagcgga tcaaaaccgg gtcgctctac gaagtcgcca
      961 cggtgttgcg caagctggtg ctgctccaga aggagcggaa cctgtccttc ggtgagaaga
     1021 agatgctgga gaacgtgcgg cagctcctgg tgagtgagat ctcgcatgcg gtcggaatcg
     1081 acgaggggaa cgcccgctcg atggtggacc gaaccatcgc caaggcctga gacctcccgc
     1141 gtggggagga ggtgatccca ggtgtcggaa tcgctcgtgc gtgtcctctt cgtgttcctg
     1201 tgcggggcgg tggggttgct gggaccggcg cttcaaggcg ggggcggcca ggccgcgatc
     1261 gcgggctcgt cgaccgggct ggtcttcgga cttttggtcg tcctgctgga gcgtcggctg
     1321 cggcacgtcc cgggcaagac catcctccag gcgctgaccg gcttcgtggt ggggctcctg
     1381 gtggggcggg tggcgtacgc cgccgtgggc aatgtcctcg ccgccttccc cgggttcgtg
     1441 gggctcttcg cggagagtct ctgcctcgtg ggtggcggct atctgggggt gctgatcgcg
     1501 ctggacaagg gtcgggaatg gaacctggcg ggcttcatcc gggtcctcaa ggagcagccg
     1561 cgggctgaga gctacaagct cctggacacc tcggtcatca tcgacgggcg gatcgccgac
     1621 atcacggaga cgggcttcct ggaagggacg ctcgtcatcc cccaattcat cctgcgggaa
     1681 ttgcagcata tcgccgactc ctccgacccg ctgaagcgga accggggccg ccgcggtttg
     1741 gacatcctcc agaagatcca gaaaaaggtg gacatccggg tcgagatcag cgacatggat
     1801 ttccccgaga tccgcgaggt ggacaacaag ctggtggcca tggcgaaggc cctgcacgcc
     1861 aagatcgtga ccaacgactt caacttgaac aaggtggcag aactgcacgg ggtcggggtc
     1921 ctgaacatca acgagctcac caacgccctg cggccggtgg tcctgccggg cgaggacatg
     1981 cgggtgtatg tcctcaagga aggcaaggaa tacaaccagg gtatcgccta cctggatgat
     2041 gggaccatgg tcgtggtgga caacgggcgc cggcacatcg gccagaccat cgacgtgtgc
     2101 gtcacgtcgg tcctccagac cacggccggg cgcatgatct tctcgcggct gaaggaagag
     2161 gcggaagccg cctaggtcgc gtagcgaacc actcccaatc tccaactccc atttcccgca
     2221 tggatcagtc gtcccctcgg gtgcgcttgg ggtctggtcg ctgggatgtg ggattttgtg
     2281 tctgaggtgt cggggtgcat gtgacggcca tcgtgccggc ggcgggcggc gggacccggc
     2341 tggcggctcc ccatcccaag caatatctgc ccctcgcggg ggttcccatg ctcacgcgga
     2401 cgctgcaggc gcttcggctc tcccgtcggg tggattgcgt gattctggtg gtgccccccg
     2461 gtcaggagat ccgctgccgg agggagatcc tggagccctt cgggttggag gtggaacccc
     2521 tggttcccgg cggtgcggac cgccaggcgt cggtctatgc cgggctgctg caggcgccga
     2581 ccgcaaccga tctgatcctg gtccacgacg gggctcgccc gctgatcacg accgcgctga
     2641 tccaggcggc cgtggcggcc gcggcggagt tcggcgccgc cgtggtcgcg gttccggtga
     2701 gcgacaccat caaacgggtc gcccccgacg gccgggtgct ggagacgccg ccgcggggac
     2761 agctctgggc cgcgcaaacc cctcaggtgt tccgcgcctc gctgttgcga gaagcccacg
     2821 aggcggcgtt gcgggacggg tttcgaggca cggacgacag cgccctggtg gagcggctgg
     2881 gccagccggc acacatcgtg ccgggcagtc cggagaactt caagatcacg acgacggccg
     2941 acctcgtcct ggcggatcag atcctccggg cgagggcggc ccagacgggg tgaggggtgc
     3001 gccagaccgg cgacctgccc ctgcgcgtcg gggtgggctt cgacatgcac cgattcgtga
     3061 cggggcgccc gcttatcctg gggggcgttc agatcccgtt cgagcgcggg ctggaaggcg
     3121 actcggatgc ggatgccctt acccacgcca tcctggacgc tctcttgggc gcggccgggg
     3181 cgggagatat cggcagccat ttcggggtgg gccgtccaga aatgcagggg atctccagcc
     3241 tgctgctcct ggaacgcgca gcggccatcg tggcggccaa aggctttcga cctggcaatg
     3301 tggatgcgac cgtggtggcc gaggcgccgc gtcttggtcc ctacatcccg tccatgcgca
     3361 aggcgctggc cgaggttctg gggcttgccg aagaccgggt gaacctgaag ggaacgacgg
     3421 caaagggaat gggggtgctc ggggccggcg agggcatcgc cgccatcgcc gtggcttccc
     3481 tgatccctga ccgggtcgga cggccggggg tcccctcgca tcggcggaaa gggattcccc
     3541 acccgcgctg agctcgccca gtgcagggtc gtgggcaatc gtttggcggc ggccattggc
     3601 ctttgaggta gatcatgacg cttcgggttt acaacacgct gacccgacgg ctcgagacat
     3661 tcgagcccct cgtcgccgga caggtgagaa tgtacacctg cgggatcacg gcgtatgacc
     3721 tctgccacat cggccacgcg cgctcggccc tggtgttcga tgggatccgg cgctacctgg
     3781 agtatgcggg ctaccgggtg acgctggtcc ggaacttcac ggatgtggac gacaagatca
     3841 tcaatcgggc ccgcgagtcg aacatccctt gggatgtggt ggcccgcgag aatatcgccg
     3901 cctttcatcg ggatatgggg gccttgggct tgcgtcccgc cgacgtcgag ccgaaggcaa
     3961 ccgatcacat ccccgagatg ctgcggatca tccaggtcct cctggagcgc gggctcgcct
     4021 acgtcgtgga cggcgatgtg tactacgccg tgcgccggtt ccccgagtac gggaagctgt
     4081 cccatcggag cctcgacgac ctcatggcgg gggcccggat cgaagtggat gagcgcaagc
     4141 gcgatccccc ggatttttcc ctctggaagg cctcccggcc tggggagccg gcgtgggaca
     4201 gcccctgggg accggggcgg ccaggctggc atatcgagtg ctcggccatg gccgccaagt
     4261 acctggggga ctccttcgat atccacgccg gcggcgagga tctgatcttc cctcaccacg
     4321 agaacgagat cgcgcaatcc gagggcgcca cggggaagcc ctttgcgcgc tattggctgc
     4381 acaacggctt cgtcacggtc aacggcgaga agatggccaa gtccctgggg aacttcgtca
     4441 ccatccggga ggccctggag cgcttcaatc ccgacgcgct gaaactcctg ctgctgtcca
     4501 cgaactaccg cgggcccctg gactacaccg aggccgccgt ggccgagaag accaaggcct
     4561 tggaaggtct tcaggagttc ttgcgggcgg tggaccgcct cacgcctccg ggatccagcc
     4621 aggcgcagat cgaggagggg tccggcggcg cccttcaccc ggccgaggca gagtttcggg
     4681 cagccatgga tgatgacttc aacaccgcca aggccacggg tgtcctcttt gacttggtgc
     4741 gagagggcaa tcggctgctc cacgaggcgg accaggacgc gtccccgcgg cctgtcagcc
     4801 tggccgccct gcaccgcacg gcagttctcc tgcgccggct gggcggtgtc ctgggtctcg
     4861 acctgcaggg cggcaggtcg gtgacatcca agctcagcgc ggtcccgctc acccgccctg
     4921 cggcggaggc cctgacggaa ctggggcagt tgctccaggc cgagggggcg gggccggcag
     4981 acctggaggc acgactgacc gccgtggtct tggaactgct ggctcaccgg gaggccgcaa
     5041 ggaaggccag ggcctggggc gaggccgatc agatccggga agcgctgtcc gcgcatggcc
     5101 tgcggatcga ggacacccgc accgcgacac atgcgatctc ggattttccc atggagcgga
     5161 ggcgtccccc catcgacgtg acggcggtca aggcgtgaag acgctccccc accggttgcc
     5221 cggaccggac gacgcggaaa tcctcgcggg tccacacgcg attctggagg ctctgcgtgc
     5281 cggccggcgt aggctgcgcc ggatcctcct ggcccgccag caggtggccg ggatcatcga
     5341 atccgtcctg gaactggcgc gcgcccgggc gttgcctgtg gaagtgaggc cgcgggcaga
     5401 gttggaccgc ctggtcccgg gggcggcgca ccaggggatc gttgccgacg ccgagccttt
     5461 cccctacgtc acgccggaag agattctctc tcaggcgcgg cggggggacg agccaggctt
     5521 cctcgtggtg ctggacggga tccaggaccc gcagaacttg gggggcatca tccggacggt
     5581 ggatgcggcg ggggcgcacg gactcatcct gccgcgagat cgtgccgcct cagtgacgcc
     5641 agcggcggtg cgagcctccg ccggggcgag tgagcacgtc tcggtggctc gcgtcacgaa
     5701 tctggccacc tttttggaat gggtcaaggg gcagggtgtc tgggtgctgg gggccgattc
     5761 gaacaccggg cgagaccttt acagcgtgga cctgacaaca ccattggcgc tggtggtcgg
     5821 cagcgaggac cgaggtctcc ggccgctggt taagaggcgc tgtgatctct tggtgcgaat
     5881 accggtgcga ggcagggtgg catcgatgaa cgcctccgcg gcggcggcca tatgtttgtt
     5941 cgaggtggtg cggcagcgga aagcggtgga aaagaggtta tgattgacag aaattcgaga
     6001 aagagagtaa aatacgaagc aatgtcaggg ttgatcaagc atcattccat gcattccaga
     6061 tgagtgccga caatccagtt tgctgtgctg gcatgcttta tatatattta gcgtttgtct
     6121 gctggcgtag ctcagcaggt agagcagctg atttgtaatc agccggtcgg gggttcgatt
     6181 ccctccgcca gctccactgc ggggacaggt gtcccgaggg gtacccagca acccgacaag
     6241 cagctcagtt gcgcccaacg cggggagata cccaagcggc caaagggggc agactgtaaa
     6301 tctgttggct catgccttcg gaggttcgaa tcctcctctc cccaccagcg tatggatgat
     6361 atggatgaat ggatgagagg ttccgcggct cgcaagccga ggttggcgag tgacggttga
     6421 aagccctccc tgggacagca ggatcgaggg cagcagggcg taaggattcg acgcaggcgg
     6481 gactagctca gctggctaga gcgcgagcct tccaagctcg gggtcgcggg ttcgaatccc
     6541 gtttcccgct ccactcgatc tctcgcgatc cgcgagaatg accatctggg caagagggtt
     6601 cgccagattc catgccatcc ctaggacccg agcgttcagg gttctggctg gtgatgacgt
     6661 tgctcgccgg gctgtgtggc ctggccatgc cggcttgggg gacgaatgcc caggcgcagc
     6721 gcctgaatga ggcggcggcg gcccgtaccg cccaggggcg atatgaggag gcggcggaac
     6781 tcttcagcca ggccctgcgg ctcagcccgg acgatccggt tatccggaag aatctggcca
     6841 ggctgcgcac ggtcatgggg catcggctgc tgagggcggg tctgctgcag cgggcacagg
     6901 agcaatatca agcggctctc gacctggacc cgaacgaagc ggcggccctc ctggggttgg
     6961 gcgacgttca gttgtggcag cgggaacccc gggccgcggc cgagagctat cgccgcgtga
     7021 tcgccttgga tccacagagt gcggatgcct atgtccgcct gggcgaagcc tataatcacc
     7081 tgggtgatat ggcggcggcc gtctcggagt gggagcgtgc cctggcgctc cggccgaatg
     7141 acgccgggct tcgccagcga ctggaccagt tgcggcgcga agagcagagc cttggcgggt
     7201 accgctcccg gggcagtcag cactttcggg tgacttacgc gggggagcgg cgggaagatc
     7261 tggggcggga gctgctgcag attctggagc gagcctacac cgatgtagga tatgagctgg
     7321 gggtgtatcc ctcccaagag gtccagacga tcttccactc ggcggacctg gtggaaggct
     7381 cctatcacct cctggacggg aagattcggg ttgcgctgag ggggcccgcg ccaggggacc
     7441 ccatgctccg cgcaatcctg taccacgaat acacgcacgc cctgatctac gcagccacac
     7501 gggcgaacaa cccgccgctc tgggtgcacg aggggttggc cgtccagatg gagaagagcg
     7561 ggcgcgcgtt gacgtacagg gatgaggcct ttcgccaggc tcgagcgggc acgccgccct
     7621 cgcccgagac gtccccgtac gtgtacgggt cggtggccat cgggtatctg atcgaccggt
     7681 acgggatgcc cgggatcggt cgcttgctgc acggaatggg agaggggctt cccttccgaa
     7741 cagcatttca ggaggctttc ggcgtcgatc tgaccacgtt ccagcagggc ttccgggacc
     7801 tgctagttcg cgggtactga atccaaacgg acaccaccca cgtagctcag ctggcagagc
     7861 gcgtccttgg taaggacgag gtcaccagtt caaccctggt cgtgggctcc agtcacatcc
     7921 cggagtgatc cgccagagtg cgggaggacg cctccatggc caaagccaag ttcgagcgga
     7981 cgaaagagca catgaacatc gggaccatcg ggcacatcga ccacgggaag accacgctca
     8041 ccgcggcgat cacgaaggcc ctgggcttga agaacccgaa g
//
LOCUS       DBNGZC010000385         4878 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1891801, whole genome shotgun sequence.
ACCESSION   DBNGZC010000385 DBNGZC010000000
VERSION     DBNGZC010000385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1891801"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1248)
                     /locus_tag="AC16G0_06840"
     CDS             complement(<1..1248)
                     /locus_tag="AC16G0_06840"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - obsolete glycogen debranching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0019156 - isoamylase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein"
                     /protein_id="HIR5099373.1"
                     /translation="MSAERVEAQIMGYELKPSTDRKVSAGAFFPLGASLTPDGVNFAL
                     YSQNASEVFLLLFNAPDGQPTDIIRLDNRTRYIWHAFVHGLTAGQLYGFKVRGDFNPA
                     RGLRFNEAKLLIDPYAKALTGKAKNQDNLLLAYDAQSPERDLSRDSRDNTAIVPKGIV
                     VDDRFDWQGDVPSCTPLENLVIYEVHLRGFTAHPSSGTTYPGTYLGFIQKIPHLTDLG
                     INAVEFLPIHEHYVEDFLLAKGLTNYWGYNTLGFFAPESSYGTRRSPGCQVSEFKTLV
                     RELHRAGIEVILDVVYNHSAEGNELGPTLSFKGIDNPTYYCLTGGPNDPGRYYLNYTG
                     CGNSLNLAHPHVIRFVMDSLRYWVEMMHVDGFRFDLASVLGREEGMFLRSASFFDAIS
                     QDPVLNRVKLIAEPWDLGTYQVGN"
     gene            complement(1354..2034)
                     /locus_tag="AC16G0_06845"
     CDS             complement(1354..2034)
                     /locus_tag="AC16G0_06845"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5099374.1"
                     /translation="MGRFFLFALLTWITGNPLLAILVLIALSVPGWWASSRWAWRLHR
                     KVRAWGEAGRLRRDLAVNPHNAAARAELGAILVRHGRFREARAELTQAMPRVDDLPEA
                     NYSLGLCLLHDGEVEKGRELVERALAINPKFGYGEPYLRLGDYRAERGEWEQAAERYR
                     QATGIHSSSVEGWYKLGQALAHLGKRDEARTALQEAVTSYRTAPWYRRADDRPWKRRA
                     RRLQRTLR"
     gene            complement(2178..2741)
                     /locus_tag="AC16G0_06850"
     CDS             complement(2178..2741)
                     /locus_tag="AC16G0_06850"
                     /inference="COORDINATES: protein motif:HMM:NF017494.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HIR5099375.1"
                     /translation="MIQLSEPYTEAKIRALKQGDMVQLSGVIYTGRDAVHKFLYDGGT
                     PPVNLQEQILYHCGPVVIKEKGQWVVKAAGPTTSIREEPYQGALIEKFRLRAVIGKGG
                     MGPKTLEACAKVGCVYLHAIGGAAQVLAACVTEVLGVSLEEFGSPEAIWAFRVKDFPA
                     VVTMDAHDNSLHAAVLEQSRERLKELV"
     gene            complement(2842..3720)
                     /locus_tag="AC16G0_06855"
     CDS             complement(2842..3720)
                     /locus_tag="AC16G0_06855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HIR5099376.1"
                     /translation="MRNLKATLLELIRRTSAELPDDVVQTIARYSKQEQPGTNAQYAM
                     NIIQQNIDMAKLKSAPLCQDTGSILFFVKAPKGFDQLAFEKAAATAVATATAKGYLRQ
                     NSVDSITGKNTGNNLGPGSPSLHFHQHRGTRLDVRLILKGGGCENVGAQYSLPDTRLH
                     ANRDLAGVRKVILDAVLQAQGKGCGPGILGVCIGGDRATGYLHSKEQLLRTLDDGNPD
                     SSLATLEKDIVASANKLGIGPMGFGGATTLLGCKIGARNRLPASFFVSISYMCWAYRR
                     QGIVLDDDGKILKWLY"
     gene            complement(3863..4510)
                     /locus_tag="AC16G0_06860"
     CDS             complement(3863..4510)
                     /locus_tag="AC16G0_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5099377.1"
                     /translation="MIHEVVVVGPLQCNCSVLGDEDTREAIVIDPGDEVERILAVLKQ
                     HNLKARYIVNTHAHFDHVGNCCDLKEATGAEIWLHKADLPIYETAPRQAALFSMYGVR
                     PIRMTAVDQYLKDADGLQVGKIAAQALHTPGHTPGSLSLHVPGDLRDILFAGDTLFSG
                     SIGRTDLPGGDLHRLLKSIKDRLLGLDDATEVWPGHGPKTTIGRERRTNPFLQGL"
     gene            4660..>4878
                     /locus_tag="AC16G0_06865"
     CDS             4660..>4878
                     /locus_tag="AC16G0_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010666214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR5099378.1"
                     /translation="MAILKVAVLGNPVLRIAAEPVKNVQAPLVQRLIDDMLETMQEYR
                     GVGLAAPQVHQSLQIAVIATEADDQASAV"
BASE COUNT          835 a         1566 c         1587 g          890 t
ORIGIN      
        1 gttgccgacc tggtaggttc ccagatccca gggctcggcg atcagcttca cccggttgag
       61 gaccgggtcc tgggagatgg cgtcgaagaa ggacgcggat ctcaggaaca tcccctcctc
      121 gcggcccagg accgaggcca ggtcgaaccg gaacccatcc acgtgcatca tctcgaccca
      181 gtaccggagg gagtccatca caaaccggat gacgtgggga tgggcgagat tcaggctgtt
      241 gccgcacccg gtatagttca ggtaataacg ccctggatcg ttgggccccc cggtgaggca
      301 gtagtacgtc gggttgtcga tccccttgaa ggacagggtg ggacccagct cgttcccctc
      361 ggccgaatgg ttgtacacga cgtccaggat gacctcgatc ccggcccggt ggagttcacg
      421 caccagcgtc ttgaactcgc tcacctggca gccgggggag cgccgggtac cgtacgacga
      481 ttccggcgcg aagaaaccga gggtgttgta cccccagtaa ttggtgaggc ctttggccag
      541 gaggaaatcc tcgacatagt gctcgtggat cgggaggaac tcgacggcgt tgatccccag
      601 gtccgtgagg tgcggaatct tctggatgaa gcccaggtag gtaccgggat acgtcgtccc
      661 ggaggagggg tgcgccgtga agcccctgag gtgcacttca tagatgacca ggttttccag
      721 cggggtacag gacggcacat caccctgcca gtcgaaccga tcgtcaacga cgatgccctt
      781 cgggacgatg gccgtgttgt ccctggaatc ccgtgagagg tccctctcgg gagattgtgc
      841 gtcataggcc aggagcaggt tgtcctggtt cttggcttta ccggtgagag ccttggcgta
      901 cgggtcgatc aggagcttgg cctcgttgaa gcgcagaccc cgggccggat tgaagtcgcc
      961 gcggaccttg aacccgtaaa gctgcccggc agtcaggcca tgcacgaagg catgccagat
     1021 gtaccgcgtc cgattgtcca gccggatgat gtcggtgggc tgaccgtccg gcgcgttgaa
     1081 gaggagcagg aacacttcgc tggcgttctg tgagtacagg gcgaagttca cgccatccgg
     1141 tgtcaggctg gcccccaggg gaaagaacgc gcccgccgag accttcctgt ccgtgctggg
     1201 cttcaactca tatcccatga tctgcgcctc cactctctcg gcggacactc ggtcttctgt
     1261 ggaaggatca gcggaatctc cctccctacc cagaaacccc ctcgcctacc ccctccccca
     1321 ccatggggga gaggaagggg atgaggggca gattcaccgg agcgtacgct gcaggcggcg
     1381 ggcccggcgt ttccaggggc ggtcgtcggc acgccggtac cagggcgcgg tgcgatacga
     1441 ggtgacggcc tcctggaggg ctgtcctggc ctcatcccgc ttgccgagat gcgcgagggc
     1501 ctggccaagc ttgtaccatc cctccacgct ggagctgtga attcccgtgg cctggcggta
     1561 gcgctccgcc gcttgctccc actcgcctcg ctcggcccgg taatccccca gccgcaggta
     1621 gggctccccg tacccgaatt tcggattgat ggcgagggct cgctccacaa gctccctccc
     1681 cttctccacc tcgccgtcgt gcagcagaca taagcctagc gagtagttcg cctcggggag
     1741 atcatcgacc cgcggcatgg cctgggtgag ttcggcgcgc gcctcgcgga agcgtccgtg
     1801 ccgcaccagg atcgccccca gctcggcccg agccgccgcg ttgtgcggat tgaccgccag
     1861 atcgcgccgg agtcgccctg cctcccccca ggcgcgcacc ttccggtgga gccgccaggc
     1921 ccagcgggag ctggcccacc agcccggcac ggagagggcg atcaggacga ggatcgccag
     1981 gagcggattc cccgtgatcc aggtgagcag ggcaaacagg aagaagcgac ccatacgcgc
     2041 gcagccctat tccaacagcc gccggtcgcg gaacccaagt tgggatcctc gcgccgcggg
     2101 gatcttcctt cggccacaac gaaaacgggg gcgaaggtct cgcccccgtc acgcacctgc
     2161 cgatcgtggt cagcccctca cacgagttcc ttcagccgct cccgggattg ttccaggacc
     2221 gccgcgtgga ggctgttgtc gtgggcgtcc atggtgacca cggcggggaa gtccttgacg
     2281 cggaaggccc agatggcctc ggggctgccg aactcctcca gggagacgcc caggacctcg
     2341 gtcacgcagg cggccaggac ctgggcggcc ccgccgatgg cgtggaggta gacgcagccc
     2401 accttggcgc aggcctccag cgtcttgggc cccatccccc ccttgccgat caccgcccgg
     2461 aggcggaact tctcgatgag ggcgccctgg tagggctcct cgcggatgga ggtggtgggg
     2521 ccggcggcct tcaccaccca ctgccccttc tccttgatca ccaccgggcc gcaatggtag
     2581 aggatctgct cctgcaggtt cacgggcggc gtgcccccgt cgtagaggaa cttgtgcacg
     2641 gcgtcccgcc cggtgtagat cacccccgag agctgcacca tgtccccctg cttcagcgcg
     2701 cggatcttgg cttcggtgta cggctcactg agctggatca tgcttgcccc ctgagaccga
     2761 atggccaatg tccaagcccc accgaccaat gaaaacggga ctcagcctga atggaaactg
     2821 gaaattggtc attggtcatt gctagtacaa ccacttcaag atcttcccgt cgtcgtccag
     2881 cacgatccct tgccgccggt aggcccagca catgtacgag atggaaacga agaagctggc
     2941 ggggagccgg ttccgggccc cgatcttaca ccccaggagg gtcgtcgccc ccccgaaacc
     3001 catgggcccg atgcccagct tgttggcgct ggccacgatg tccttttcca atgtcgcgag
     3061 cgatgagtcc ggattcccgt cgtccaatgt gcgaagcaac tgctccttcg aatggaggta
     3121 tcccgtcgcc cgatcgccgc cgatgcagac gcctagaata cccggtccgc agcccttccc
     3181 ctgggcctgg agcacggcgt ccaggatgac cttacgcacg ccggccaggt cccggttggc
     3241 gtggaggcgc gtatcgggga gagaatactg ggctcccacg ttctcgcagc cgccgccctt
     3301 caggatgagc cgcacatcca ggcgggtccc ccggtgctgg tggaagtgga gactcgggct
     3361 gcccgggccc aagttgttcc cggtattctt cccggtgatc gaatccaccg agttctggcg
     3421 gagatagcct ttcgccgtgg ccgtggccac cgccgtggcc gcggctttct cgaaggcgag
     3481 ctgatcgaaa cccttgggcg cctttacgaa gaacaggatg ctcccggtgt cttggcagag
     3541 cggcgccgac ttcagcttcg ccatgtcgat gttctgctgg ataatattca tggcgtactg
     3601 ggcgttggtg cctggctgct cctgcttgga gtagcgggcg atcgtctgga cgacgtcatc
     3661 cggcagctcg gccgaggtgc ggcggatgag ctccagcagg gtggctttca ggttccgcat
     3721 gatggtctcc cttctgccgg gcgcggcccg acggcgttga gggctggatt ctaggtccga
     3781 cgccgggtcg ctgtcaatgc ggacaagcgg ggggtgtgcg ttggcagtca aatgctggtg
     3841 tgctacactg cggcccgtag tttcagagtc cctggaggaa agggttggtc cggcgctctc
     3901 gcccaatcgt ggtcttgggg ccgtggccgg gccagacctc cgtggcgtcg tccaggccga
     3961 gcaagcggtc cttgatggac ttcagaagcc gatggagatc gccccccggc aggtcggtgc
     4021 ggccgatgct gccgctgaaa agggtatcac ccgcgaagag gatgtcccgg aggtcgcccg
     4081 ggacgtgtaa gctgagactg ccgggggtat gccctggtgt gtggagggcc tgggctgcga
     4141 tcttcccgac ctggaggccg tccgcgtcct tgaggtactg gtccaccgcg gtcattcgga
     4201 tgggccggac accgtacatg ctgaagagcg ccgcctgccg cggggcggtt tcgtagatgg
     4261 gcaggtcggc cttgtgaagc cagatctccg cccccgtcgc ctccttcagg tcgcagcagt
     4321 tgcccacgtg atcaaaatgg gcgtgggtgt tcacgatgta cctagctttc aggttatgct
     4381 gcttcagaac cgccaggatg cgctcgactt cgtcgccggg gtcgatgacg attgcttccc
     4441 gggtatcttc atcgcccagg accgagcagt tgcattgcag gggaccgacc accaccactt
     4501 cgtgtatcat tggacctccg aaacctatcg gatgacaaat tgcggaatgc ggattgtcgg
     4561 taccggacgt ccgatgtccg gggcgctcgc cggtcgacat tcgaaaatct cggcaatgtc
     4621 aagctagcac aacaacccgc ggaaaggaac agtcgcacca tggccattct caaggtcgcg
     4681 gtactcggca acccggtcct gcggatcgcc gccgagcccg tgaagaatgt ccaggcccct
     4741 ctagtccagc gcctcatcga cgacatgttg gagaccatgc aggagtatcg cggcgtgggg
     4801 ctggcggcgc cccaagtcca tcagtctctc cagatcgcgg tgatcgcgac cgaggcggac
     4861 gaccaggcct ccgcagtc
//
LOCUS       DBNGZC010000386         1358 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1303487, whole genome shotgun sequence.
ACCESSION   DBNGZC010000386 DBNGZC010000000
VERSION     DBNGZC010000386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1358)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1358)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1358
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1303487"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="AC16G0_06870"
     CDS             complement(<1..653)
                     /locus_tag="AC16G0_06870"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1/NuoH family protein"
                     /protein_id="HIR5099379.1"
                     /translation="MLEAIIIMLIKTGVVFGALLLTVAYMTWIERKVIADIQVRLGPM
                     RVGFHGLMQPVADGIKLMFKEDLIPSHVDRTLFLMAPALSIIPAFIAFAVIPIGNTTT
                     LFGLLSKPVPLYITDINVGVLYVFGITSLGVYGIVLAGWSSNNKYSLLGGLRSSAQMI
                     SYELSMSLSVVGILMLVSSLSLVDIVKAQAGFSWWLGALPVPNWFVFSQPLGFIIYF"
     gene            complement(734..>1358)
                     /locus_tag="AC16G0_06875"
     CDS             complement(734..>1358)
                     /locus_tag="AC16G0_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR5099380.1"
                     /translation="REILRTPHLDFRVRPLQFQDADAAEDGILRRTDKTANSRGARDL
                     SILPGRDGLDTLGMIAAAAEGRLKALFVFEEDLVRELPDRQVRNALGKLDLLVTSSLF
                     PTETTALAHVVLPALGFAEKEGSFTNHQGRIQKIHGALTPPGTSRSLPEVLRDLAREL
                     DWDLGDIEPERVWAAIGDMPGPYQGIGWREWKEIGALGLLPGEQRGK"
BASE COUNT          247 a          451 c          433 g          227 t
ORIGIN      
        1 ctgaaataga tgatgaatcc cagcggctgg ctgaacacga accagttggg gaccggcagg
       61 gcgcccaacc accaggagaa gccggcctgg gccttcacga tgtccaccag gctcagggag
      121 ctcaccagca tcaggatgcc caccaccgag aggctcatgg acagctcgta gctgatcatc
      181 tgggcggagg agcgcagccc gcccaggagc gagtacttgt tgttggagga ccagcccgcc
      241 aggacgatcc cgtacacccc gagggaggtg atgccgaaga catacaggac gcccacgttg
      301 atgtcggtga tgtagagcgg cacgggcttc gacaggaggc cgaagagcgt ggtggtattg
      361 ccgatgggga tcaccgcaaa ggcgatgaag gccggtatga tgctgagggc cggggccatg
      421 aggaacagcg tccggtccac gtggctcggg atcagatcct ccttgaacat cagcttgatg
      481 ccgtcggcga cgggctgcat gaggccatgg aaccccacgc gcatcggccc caggcggacc
      541 tggatgtcgg cgatgacctt tcgctcgatc caggtcatgt aggccacggt caggagcagg
      601 gcgccgaaca ccaccccggt cttgatcagc atgatgatta tggcttcaag catttccgct
      661 ctcagccttc agcggtcggc cccgcctctc cgacggcgat ttccaaccga tgtggctgtc
      721 ccactcgctg ccttcacttg ccgcgttgct cgcccggcag gagtcccaac gccccgatct
      781 ccttccactc cctccagccg atcccctgat acggtcccgg catgtcgccg atggcggccc
      841 agacccgttc cggctcgatg tcgcccaggt cccagtccag ctctcgggcc aggtcccgaa
      901 gcacctccgg caggctccgg gaggtgccgg gcggcgtgag ggcgccgtgg atcttctgga
      961 ttcgaccctg atgattcgtg aagcttccct ccttctcggc gaaccccagg gccggcagaa
     1021 caacgtgagc caaggccgtg gtttcggtgg gaaagaggct gctcgtcacc aggaggtcca
     1081 gcttgcccag ggcattccgg acctggcgat ccggcagctc tcgcaccaga tcctcctcaa
     1141 acacgaacaa ggccttcagc cgcccctccg ccgcggccgc gatcatcccc agggtgtcca
     1201 ggccatcgcg acccggcagg atggacaggt cgcgcgcccc gcggctgttg gccgtcttat
     1261 cggtgcgccg caggattcca tcctcggcgg catcggcgtc ctggaattgc aggggccgga
     1321 cacggaaatc gagatgcggc gtccggagga tctcccgg
//
LOCUS       DBNGZC010000387         8250 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1401982, whole genome shotgun sequence.
ACCESSION   DBNGZC010000387 DBNGZC010000000
VERSION     DBNGZC010000387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 8250)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8250)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8250
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1401982"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1753
                     /locus_tag="AC16G0_06880"
     CDS             <1..1753
                     /locus_tag="AC16G0_06880"
                     /inference="COORDINATES: protein motif:HMM:NF017197.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR5099381.1"
                     /translation="EAKGIQVAQAQGFGAEPFLTADALRVHVQVLPLFRGQIKVTTVV
                     LERPRIRLARARDGRWSVEDLLKAPPAPAPAKPPGEAPHPGKAPLFGGLLLSEVAVQD
                     GEISLLDQARPKPITLSLTNLDLSLKEASPSSPIDFRLQARITGSGTGRIESAGRIIL
                     GEQEGPVLDASIGLRDLDAASWQGLLGGEGVTLSGPLSAEIKLTGHPAKAAFSGIVNL
                     KGVAIQAGSTFKKAAGEEAAVRFQGRREDPGVTLSKLLLTLKDVTVDGSLRIPDLKSP
                     KVTFDATSEKVDLDRLLSPPKAKTTWLGPVPAHAVPTPGPGIEGKAFGSGLTAQGRVK
                     IGDLRYQGLVWTAVSADIRYQDGLVQLPDLQADFMNGKIAAKGELDIRPKTPSVTLTS
                     RVSDVATEPLVKALALGSWSLKSILGTDSSLSFVGFSRPAILGSANGSGSILLKDGRL
                     SNYKPLDRLSEVLGPLMAAQGVKVRLDEFDQLSGHYTLDKGVLRTKDLVLTKTEGTVT
                     AVGALGLLDSSLDFDVVAKLGRSVVEAKVTGTTDKPIVVPKLARFQQKIETEINKALP
                     GEQGKGLKDLFKGLFGK"
     gene            1785..2822
                     /locus_tag="AC16G0_06885"
     CDS             1785..2822
                     /locus_tag="AC16G0_06885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5099382.1"
                     /translation="MRLALVTPHYYPSVRGNSVTVQRIESGLRDQGVTIQVFSLDQET
                     PDAILTGLQRFLPDAIHGFHATKAGPLTIQAAHTLRIPAIITLTGTDVNLDLVDAGRR
                     PIVLAVLQAAQAIVAFHETIRAKVEREAPDVGLRIRVIGQTVQCDEARYDLRGKLGLG
                     PRNFVIFQPAGIRRVKNIPSVIPPLRTLQERYPHLRYVLAGPVIEQGEAARVQGLLRD
                     LPWAFFLGAVTHEEICAILPQVEVVVNSSLSEGGMSNAVLEAMSKGVPVVASDIDGNR
                     SVILDGQDGFLFGSEAEFVQKMGRLLDDAALRYALGRRAKQKIAAHFKLEGEIGGYLA
                     LYRSLVAGEGG"
     gene            2828..3730
                     /locus_tag="AC16G0_06890"
     CDS             2828..3730
                     /locus_tag="AC16G0_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HIR5099383.1"
                     /translation="MDLRALEVFCKIMELKSFSRAAEAVLLTQPTVSGHIKALEEELG
                     LRLFDRAGKSVTPTRAGELLHGYARRILALREEAQQAISEHKGGLKGHLAVGGSSIPG
                     AYILPPLLAAFKQDHPEVTIGLHVSGSHDIIRSVIEGAAELGMVGARFEEGRVQYEPY
                     AQDELVLAVAASHPWARRSTVRPTELAGQVFVMRERGSGTRKVMEEVFARHDLDPGDL
                     RVALEVTGNDAVRQAVKAGAGVTVISRRAIEDDVRCKILVALRLQGLRLVRDVFLVTH
                     KSRSRSPLGKAFLSFLQQSAQASL"
     gene            3784..4404
                     /locus_tag="AC16G0_06895"
     CDS             3784..4404
                     /locus_tag="AC16G0_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIR5099384.1"
                     /translation="MVEVILSGWLIGVAIALPVGPVITELIRRGLRGGFLQGWLVGLG
                     AAASHVILVSLTLVGVVAFLDRPIWHTILGGAGTLVLGYLGLDALRASTMPPGPADGG
                     RGGHPFLAGFGIGISNPITLLWFLTVGGALITAHGGGRTGLLAVLFGVSFIFGVLSWD
                     TVVAGVAGYGRRWMGPRTLKALNVTAGLVFFGFALRLLWRLAQTLR"
     gene            complement(4431..5096)
                     /locus_tag="AC16G0_06900"
     CDS             complement(4431..5096)
                     /locus_tag="AC16G0_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RQC-minor-1 family DNA-binding protein"
                     /protein_id="HIR5099385.1"
                     /translation="MGRKVRRVGVQLDSKEITSLPGDELRAILRGADDLIMQGGRSLL
                     VKVLKGSRAQEVLKLGLDQSPVYGYYRQLSPEEILARIDWVIEAGYLDIQYDGRLPLL
                     AYTEPGWDIERETYAEELLRGFDKMLAAGPGPFDMSYLKDRNRGMILLLLDKVEATGD
                     RKYIPLLHAWEQADYKKVQARIRQVIRHLQQSAPHDAVAANSLSHEERDTVEYGKLSG
                     ESD"
     gene            5408..6382
                     /locus_tag="AC16G0_06905"
     CDS             5408..6382
                     /locus_tag="AC16G0_06905"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF048461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HIR5099386.1"
                     /translation="MSANPERGTKLFQYLDVPGAATERNYVNNPLFGPPRPRTATIPH
                     MTVNGAKDGPTLCVMAGMHACEYPSIDAAIQTYRNTDPRTLAGRLIIVPVVNLPAFWT
                     RTPYLNPHDMMDISATYHLEGSSISFLIGRLLMEQVFAKADYIVDLHGGDVMEDVVPH
                     AGFTKIGDKEIDEKSEMLARAYGTRYVFERLEKGAFEKSGIRKPRTLAEAGREGRLEP
                     ELTAVHLSGIANIMKSLKMIEGKPEIPADQVVLHGRYEIFCRTAGLFYPRIKMGDRVK
                     RGDLLGEIRDLEGKILEQIVAPRDSVVLLMMTNPVKHPDDLVFKMWLY"
     gene            complement(6519..7649)
                     /locus_tag="AC16G0_06910"
     CDS             complement(6519..7649)
                     /locus_tag="AC16G0_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR5099387.1"
                     /translation="MRIERIEIFPFEIPYREGLDVTTSLGRHSSLRNILLGLYADTGE
                     VGWGEAAPLPTHSGETQESILALLRHTLGPMLLGRDPRDIPVLLGEMDRLLFGQTFSK
                     SAVDYALHDLVAKRCGIPLYQLLGGKCRETFPLGWTIGWKPLETTLAEAAEAVRLGFK
                     AIKLKVGNPDWRTDLERVREVRKVVGESVPIRVDANQGYTPHAAVEVVQRMADCSLQL
                     VEQPVRRWDLEGLAFVRQRIPFPVMADEAAFTPNDIVRIARLSAADIINIKPQKFGGL
                     ARSREIGAIAAAAGMEVFASSRMCSGVGVAAAVHFYAALPGAAYEGEFVDGILLAEDD
                     LLAEPIEVRDGLVRVPAGPGIGVSLHRGKLERYAASPVVLTA"
     gene            complement(7666..>8250)
                     /locus_tag="AC16G0_06915"
     CDS             complement(7666..>8250)
                     /locus_tag="AC16G0_06915"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099388.1"
                     /translation="GAVAVAVLGGVLLGVAGGLSRTIGFLLMRLVDIVICFPPIIVAI
                     FFMAMAKPGVLTLILVIGFLYAPRMARVVQSAVLEVAQAIHVEAQRALGAGTARIVWK
                     GILPQILAPIVVQATIMLATAMLLEAGLSFLGLGIPPPTPTWGQLVSDARRTVHLSLY
                     LLLFPGLALSANVIFINLLGDGLRDFLDPKMRRR"
BASE COUNT         1469 a         2676 c         2723 g         1382 t
ORIGIN      
        1 cgaggcgaag ggcatccagg tggctcaggc gcaggggttc ggggcagagc cgttcctcac
       61 cgcggacgcc ctccgcgtcc atgtgcaagt gctccctctg ttccggggcc agatcaaggt
      121 gaccacggtg gttctggagc ggcctcgcat ccgcctggcg cgcgcccggg acggtcgctg
      181 gagcgtggag gatctgctga aggctccccc ggccccggcg ccggcgaagc cgccgggtga
      241 ggctccgcat cccgggaagg cgccgctctt tggcggactg ctcctctcgg aagtcgccgt
      301 gcaagacggc gagatctccc tcctcgacca ggcccggccg aagccgataa ccctcagcct
      361 cacgaacctg gatctcagtc tcaaggaggc gtccccatcc agcccgattg acttccggct
      421 gcaggccagg atcacgggca gcggaacggg gcggatagag agcgcgggcc ggatcatatt
      481 gggcgagcag gaaggccccg tcctggacgc cagcatcggt cttcgcgacc tggacgcagc
      541 gtcctggcaa ggcctcctgg gaggggaggg ggtgacgctc tccggtccgc tctcggcaga
      601 gatcaaactg accggccatc ccgccaaggc tgcgttctcc ggcattgtga acctgaaagg
      661 ggtggcgatc caggcgggtt caaccttcaa gaaggcggcg ggagaagagg cagcggtacg
      721 ctttcaggga cggcgagagg atccgggagt gaccttgtcg aagctgcttc tcacgctgaa
      781 ggacgtgacg gtggatggca gccttcggat cccggacctc aagtccccca aggtcacgtt
      841 cgatgcgacc tccgagaagg ttgacctgga tcggctgcta tcgccgccca aggcgaagac
      901 cacctggctg ggtccggtac cggctcatgc ggttccaacg cccggaccgg ggatcgaggg
      961 aaaggctttc gggtcgggac tcacggccca aggccgggtc aagatcggcg acctgcgcta
     1021 ccaagggctg gtgtggacgg ccgtcagcgc cgacatccgg taccaagatg gcctggtcca
     1081 gttaccggac ctccaggccg acttcatgaa cgggaagatc gcggccaagg gggagttgga
     1141 tatccggccg aaaacgccga gcgtgacgct cacctcgcgg gtctccgacg tggccacgga
     1201 gcccctggtg aaggcgctgg ctctgggctc ttggtcgctc aagagcattc tcggaaccga
     1261 cagcagcctg agcttcgtcg ggttctcccg gcccgctatc cttggatcgg cgaacggtag
     1321 cggatccatc ctgctcaagg acgggcgcct cagcaactac aaaccccttg accggctgtc
     1381 cgaggtgctc ggcccgctca tggcggccca gggcgtcaag gtgcggctgg acgaattcga
     1441 ccagctcagc ggccattaca cgctggacaa gggggtcctc aggacgaagg acctcgtgct
     1501 caccaagacc gagggaacgg tgacggccgt gggggccctg ggacttttgg attccagcct
     1561 ggatttcgac gtggtggcga agctgggccg ctccgtggta gaggccaagg tgaccggcac
     1621 gaccgacaag cccatcgtcg ttcccaagct ggccaggttc cagcagaaga tcgagaccga
     1681 gataaacaag gccttgcccg gcgagcaggg caaggggctg aaggacctgt tcaagggatt
     1741 gttcggcaag taaccgcgac gcggggcccg cgcatcccaa cagcatgcgg cttgctctgg
     1801 taacccccca ctactacccc tcggtccgcg ggaattccgt cacggtccag cggatcgaat
     1861 cgggcctccg cgatcaggga gtgaccatcc aggtcttctc ccttgatcag gagacccccg
     1921 acgccatcct gacgggtctg cagcgtttcc tccccgacgc gatccacggt ttccacgcca
     1981 ccaaggccgg cccgctgacg atccaggcgg cccacaccct ccgcatcccg gcgatcatca
     2041 cgctcaccgg tacggacgtg aacttggacc tggtcgacgc cggacgccgg cccattgtcc
     2101 tcgcggtcct gcaggccgcg caggccatcg tcgcattcca cgagaccatc cgtgccaagg
     2161 tagagcgcga ggcccccgac gtcggcctgc gcatccgggt catcggccag acggtccagt
     2221 gcgatgaggc acgctacgac ctgcggggta agctgggcct tggccctcgc aatttcgtca
     2281 tcttccagcc cgcggggatc cggcgtgtga agaatatccc gtcggtgatc ccgccgctcc
     2341 gcacgctcca ggaacgatac ccgcacctcc gctacgtcct ggcgggtccg gtgatcgaac
     2401 aaggcgaggc agcgcgggtg cagggattgc tgcgcgactt gccttgggcc ttctttctcg
     2461 gggcggtgac gcacgaggag atctgcgcga tcctgcccca ggtggaggtg gtggtcaact
     2521 cctctctctc cgagggcggg atgtccaacg cggtcctgga ggccatgagc aagggggtgc
     2581 cggtcgtggc ctcggatatc gacgggaatc ggtcggtcat cctggacggg caggacgggt
     2641 tcttgttcgg ctcggaggcg gagttcgtgc agaagatggg acggctcctg gatgacgcgg
     2701 ccctccgcta tgccctgggc cgccgggcca agcagaagat cgcggcccac ttcaagctgg
     2761 agggagagat tggtgggtac ctcgccctct accggtccct cgtcgccggg gaaggaggct
     2821 aggaccggtg gacctgcggg cgctggaggt cttctgcaag atcatggaac tcaagagctt
     2881 ctcccgggcg gccgaggcgg tcctgctcac ccagcccacg gtgagcgggc acatcaaagc
     2941 cctggaggag gagctgggcc tccgcctttt cgatcgggcg gggaagagcg tgacgcccac
     3001 gcgggccggg gagctcttgc acggctacgc gcgacgaatc ctggcgctcc gtgaggaggc
     3061 ccagcaggcc atcagcgagc acaagggggg tctgaagggc catctggccg tcggggggag
     3121 cagcatcccg ggggcctaca tcctcccgcc gctcctggcg gccttcaagc aggaccaccc
     3181 ggaggtcacc atcgggctgc acgtgagcgg ctcccacgac atcatccgca gcgtgatcga
     3241 gggggcggcc gagttgggca tggtgggcgc gcggttcgag gaggggcggg tgcagtacga
     3301 gccctacgcg caggacgagc tggtcctggc cgtggcggcc tcccacccgt gggcccgccg
     3361 cagcacggtg cgaccgacgg agctggccgg ccaggtgttc gtcatgcgcg agcgaggctc
     3421 gggtacccgg aaagtcatgg aggaggtctt tgcccgccac gacctggacc cgggcgatct
     3481 ccgcgtggcc ctggaggtca cgggaaacga tgccgtccgc caggcggtca aggccggcgc
     3541 gggggtgacg gtgatctccc gccgggccat cgaggacgat gtccgctgca agatcctcgt
     3601 ggcgctgcgc ctccaggggc tcaggctggt gcgggatgtc ttcctggtga cgcacaagag
     3661 ccggtcacgc tctcccttgg ggaaggcctt cctctcgttt ctgcagcagt cggcccaagc
     3721 ttccctctga tctcgacctt cgatcccaag gcccgagtcc ccatcccagc gacgggagaa
     3781 gccatggtcg aggtgatcct gagcggctgg ttgatcggcg tggccattgc cctgccggtt
     3841 gggccggtca tcaccgagct gatccgccgg ggattgcggg gcgggttcct tcaagggtgg
     3901 ctagtcgggc tgggtgccgc cgcgtcgcac gtgatcctgg tctccctcac cctcgtgggc
     3961 gtggtggcgt tcctggatcg gcccatctgg cacaccatcc tgggtggcgc cggcaccctg
     4021 gtcctgggct atttgggcct ggatgccctc cgcgccagca cgatgccgcc gggtccggca
     4081 gatggggggc gaggcggcca tcccttcctg gccggattcg gcatcgggat cagcaacccc
     4141 atcaccctgc tgtggttcct gacggtcggc ggggcgctca tcacggcgca cggcgggggc
     4201 aggacaggcc tcctggctgt gctcttcggc gtgagcttca tattcggggt gctttcctgg
     4261 gatacggtcg tggcgggagt ggcggggtat ggtcggcgct ggatgggtcc caggacgttg
     4321 aaagccctga acgtcaccgc cggcctggtc ttcttcggct tcgccctccg cctgctgtgg
     4381 cgactcgctc agaccctgcg gtaggccccc cttctttaag gcacatcaat tcaatcgctc
     4441 tcgccggaca acttcccata ctctacggtg tcccgttctt cgtgtgacag actgttggcc
     4501 gcgaccgcat cgtggggcgc agactgttgg agatggcgaa tgacctgccg gattctcgcc
     4561 tggaccttct tgtagtcggc ttgctcccag gcatgcaaca gcggaatgta cttccgatcg
     4621 ccggtggcct cgaccttgtc aagcaagagc agaatcatgc cgcgattccg gtctttgagg
     4681 tagctcatgt cgaaggggcc cgggcccgct gccagcatct tgtcgaagcc gcgcagcagt
     4741 tcctcagcgt aggtttccct ctcaatgtcc catcccggct cggtgtaggc gagcaagggc
     4801 agccggccat catactggat atccagatag cctgcctcaa tgacccaatc aatccgggcc
     4861 agaatttctt ctggggacaa ctggcggtag tagccataca cagggctctg gtccagccct
     4921 aatttcaaca cctcctgggc tcgcgagccc ttcagcactt tcacgagaag agaccgcccg
     4981 ccctgcatga tcaggtcgtc ggcgccccgc aagatggctc tgagctcgtc gccggggaga
     5041 ctcgtaatct ctttcgagtc aagctgaacc ccgacccgcc ttaccttgcg ccccattggt
     5101 ctccccgtcc cctcgcccgt ggcaaaaacc gaaaaactgt gccgaggaaa tcaagcagtc
     5161 atcccctcaa gtgtccggta ctttctcctg caactcaggt gcagcaccag gatggccaat
     5221 atctccttcg gcagcctctg acgatccaac gggatccgca aatgcgcctc cgcgcgcctt
     5281 cgccggcgtt gggtttctcc ttgacaagct ttctgcattc aggcataggt caatggccgc
     5341 tgaagccaca tcgctgctct ttagccaggc gacatccctt gccgcaccac aacgatggag
     5401 gaagacaatg agcgcaaacc cggagcgagg caccaaactt tttcagtacc tcgatgtccc
     5461 tggggctgcg acagaaagga attacgtcaa caatccgctc tttggccccc cgcgaccgag
     5521 gaccgccacg atccctcaca tgaccgtgaa cggggcgaaa gacgggccga ccttgtgcgt
     5581 catggcggga atgcatgcct gcgagtaccc ctccattgac gcggccatcc agacctacag
     5641 gaataccgat ccgcgcacgc tagccggacg tctgatcatc gtccctgtgg tgaacctccc
     5701 cgccttctgg acgcgcactc cgtacctcaa tccccacgac atgatggata tcagcgccac
     5761 ctaccacctg gagggaagct ccatcagctt cttgatcggt cggctgctga tggaacaggt
     5821 gttcgcgaag gcggattata tcgtggatct gcacgggggc gacgtgatgg aagatgtcgt
     5881 accccatgcc ggattcacca agatcggcga caaggagatc gacgagaaat ccgagatgct
     5941 cgcccgagcc tacgggacga gatacgtgtt cgagcgtctg gagaaggggg ccttcgagaa
     6001 gagcggtatc cggaagccgc ggacactggc agaagctggg cgggaaggcc ggctcgaacc
     6061 ggaactcacc gccgtccacc tcagtggcat tgcgaatatc atgaaatccc tcaagatgat
     6121 cgaggggaag ccggagatac ctgccgatca ggtggtcctg cacggacgat acgagatctt
     6181 ctgccgcacc gcgggcctgt tctatccgag gataaagatg ggggaccgcg tgaagcgggg
     6241 ggacctcctc ggcgaaatcc gtgacctcga aggcaagatc ctcgagcaga tcgtcgctcc
     6301 cagggacagc gtggtgctgc ttatgatgac caaccccgtg aagcatcccg acgacctcgt
     6361 gttcaagatg tggctgtatt gaatcgagaa gccacaccgc cgacgcgaag gaaatcaaga
     6421 cgccgtgcgg ccgcccgcag ggcgtggtat ctcttctcga agcgaggagc aatccgccac
     6481 caacgtccga tctcgatgcg ccgcttgggg tcggccggtc acgccgtgag gacgacgggg
     6541 gaagcggcat accgttcgag ctttccgcga tggagcgaga ccccgatgcc gggtccggcc
     6601 gggacgcgaa ccaggccgtc ccgcacctca atcggctccg ccaaaagatc atcctccgcc
     6661 agcaggatac cgtccacaaa ctccccttcg tacgccgcgc cggggagcgc cgcgtagaag
     6721 tgaacggccg ccgccacgcc gaccccggag cacatgcggg agctggcaaa gacctccatt
     6781 cccgccgccg cagcgatggc cccgatctcg cgggatcggg ccagcccccc aaacttctgg
     6841 ggcttgatgt tgatgatgtc ggccgccgaa aggcgcgcga tccgcacgat gtcgttggga
     6901 gtgaaagccg cttcatccgc catcaccggg aaggggatgc gctgccggac gaaggctagc
     6961 ccctccaggt cccagcggcg gaccggttgc tcgaccagct ggaggctgca gtcggccatg
     7021 cgctgtacga cctcgacggc agcgtgcggc gtgtacccct gattggcgtc cacgcggatg
     7081 ggaacgctct cccccacgac cttccgcacc tcccggaccc gctccagatc cgtgcgccag
     7141 tccggattgc cgactttgag cttgatggcc ttgaagccga gccgcacggc ctccgccgcc
     7201 tcggcgagcg tcgtctccag gggcttccag ccgatggtcc agcccagggg gaaggtctcg
     7261 cggcacttgc cgcccaggag ctggtagagg ggaatgccgc agcgcttcgc caccaggtcg
     7321 tgaagggcgt aatccaccgc gctcttggaa aaggtctggc caaacaggag ccggtccatc
     7381 tcaccaagca gcacgggaat gtcacgggga tcgcggccga ggagcatcgg gccgagggtg
     7441 tgcctgagca gggcgaggat gctctcctgc gtctcgcccg agtgggtggg gagcggggcg
     7501 gcctcccccc agcccacctc gcctgtgtcg gcgtaaaggc cgagcaggat gttccggagc
     7561 gaggagtgac ggcccagcga tgtggtgacg tccagtccct cgcggtaggg gatctcgaag
     7621 gggaagatct caatccgctc gattcgcatg cccagtcctt tggcctcagc gccgccgcat
     7681 cttggggtcc aggaaatccc gcaggccgtc tcccagcaag ttgatgaaga tcacgttggc
     7741 cgagagcgcc agacccggga acaggagaag gtacaggctc aggtggaccg tgcggcgggc
     7801 gtcgctcacc agttggcccc aggtcggggt cggtggcgga atgccgagcc cgaggaagga
     7861 gagcccggcc tccagcagca tggcggtcgc cagcatgatc gtcgcctgca ccacgatggg
     7921 agcgaggatc tggggaagga tccccttcca gacgatgcga gccgtccccg cgccgagggc
     7981 ccgctgggcc tccacgtgga tggcctgggc gacctccagg acggcgctct ggacgacacg
     8041 ggccatgcgg ggggcgtaca ggaatccaat caccaggatg agggtgagca ccccgggttt
     8101 cgccatggcc atgaagaaga tggccacgat gatgggggga aagcagatga cgatgtccac
     8161 caggcgcatc agcagaaagc cgatggtccg ggacagaccc cccgcgacgc cgagcagcac
     8221 ccctcccagg acagccaccg ccacggcgcc
//
LOCUS       DBNGZC010000388         1590 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1402051, whole genome shotgun sequence.
ACCESSION   DBNGZC010000388 DBNGZC010000000
VERSION     DBNGZC010000388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1402051"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..909)
                     /locus_tag="AC16G0_06920"
     CDS             complement(4..909)
                     /locus_tag="AC16G0_06920"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HIR5099389.1"
                     /translation="MRAHPRSRDTWTPRPGARGASLVELVIVMAITGIITTGLYAFFV
                     STSNTYSDQAVVARMLQTAGIAMTRISQDIRKAGTFWSLPCAVETLVTGTNGPPGNIE
                     IRLMLDDPAARTEVAPPPPASQSQSSSVLRVLSTTGFQDGDTAFITDGVQCSRFAVTQ
                     IIGGANPGLQHNPSDDLNSPGGFNYLYPATTSMVYRLGVSQRVTYSIDTSDPVTPWLT
                     RDTGAGPRRLLPDIESLRLSYVMADGSIVSDPSTITTAAQAANIRLVTVSVTATADRR
                     SPGIGGDGFRRQTLASRVQLRNLGQ"
     gene            complement(906..1271)
                     /locus_tag="AC16G0_06925"
     CDS             complement(906..1271)
                     /locus_tag="AC16G0_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification PilV family protein"
                     /protein_id="HIR5099390.1"
                     /translation="MSIAEVLVAIVFIAVFAAGMAPLAITVVTANRQAKSTDIAVYLA
                     HDRLETIRNTAYASLTGANFPNEGYGTIAIGSPAVAYPDYQRSTSVQDNTPTAGMKRV
                     VVTVSWRGGSVSEEILVGQ"
BASE COUNT          269 a          494 c          521 g          306 t
ORIGIN      
        1 tggctattgc ccgaggttac ggagctgcac gcgcgacgcc aaggtctggc gacggaaccc
       61 gtcgccgccg atgccggggc tccggcggtc ggccgtggcg gtcacgctca cggtcaccag
      121 gcgaatattg gcggcctggg ccgcggtcgt gatggtggat ggatcgctca cgatcgagcc
      181 gtcggccatc acgtacgaca ggcggaggct ctcgatgtcg ggcaggagcc gcctggggcc
      241 ggccccggtg tcgcgtgtca accagggcgt gacggggtcg gatgtatcga tgctataggt
      301 gacccgctgg ctgacgccga gccggtacac catgctggtt gtcgcggggt agaggtaatt
      361 gaagcccccg ggagagttga ggtcatcgct ggggttatgc tgcaaaccag gattggcgcc
      421 gccgatgatc tgggtgacgg cgaagcgcga gcactgcacc ccgtccgtga tgaacgcggt
      481 atccccgtcc tggaatcccg tggtggagag gacccgaagg accgagctgc tttgcgactg
      541 actggccggg gggggcgggg ccacctcggt tcgtgccgcg ggatcatcca gcatgagtcg
      601 gatctcgatg ttgccgggcg gaccgttggt tcccgtgacc aaggtctcca ccgcgcacgg
      661 gagcgaccag aacgtcccag ccttccgaat atcttggctg attcgggtca tggcgatccc
      721 ggcagtctgc agcatgcgtg cgacgacggc ttggtcgctg tacgtgttcg acgtcgagac
      781 gaaaaaggca tagaggcccg tggtgatgat gccagtgatc gccataacaa tcacgagctc
      841 caccaggctc gccccccgtg cgcctgggcg aggcgtccag gtatcccttg atcgcgggtg
      901 tgccctcatt gccccaccaa gatctcttcg ctcacacttc cgcctcgcca ggatacggtg
      961 accacgactc gcttcatccc cgccgtgggg gtgttgtcct gaacgctggt cgatcgttga
     1021 tagtccggat aggcgacggc gggactcccg atcgcgatgg ttccgtatcc ctcgttcgga
     1081 aagttcgccc ccgtgaggct cgcgtaggct gtgttccgaa tggtctctag tcgatcgtgg
     1141 gccaggtaca ccgcgatgtc cgttgacttc gcttgccggt ttgcggtcac gacggtgatt
     1201 gccagcggcg ccatcccggc ggcaaagaca gcgatgaaaa caatcgccac gaggacctcg
     1261 gcgatgctca cccctggcat ccccgcgcac cgaggactgt ctgacgcagg ttgaggggag
     1321 gggtgatcgg tcttgacagg ttggcaggat ggaccacgca ttcgatcgtc ctcaggccgc
     1381 gggaaaggtc tgaatttgga ccactccatc cgacctcagt gtttcgacag aggcctttgg
     1441 gtcagaatac aagcacatcc tgtgccattc gaggtcgaac tccgcggccc acccagcctt
     1501 cgagcaactg cttcccccga tattctcccg cctcagaccc tgcagaccgc gaagtccttt
     1561 ccccattgcg ccgtgccagc atcttcgtcg
//
LOCUS       DBNGZC010000389         4117 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2183944, whole genome shotgun sequence.
ACCESSION   DBNGZC010000389 DBNGZC010000000
VERSION     DBNGZC010000389.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2183944"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="AC16G0_06930"
     CDS             complement(<1..402)
                     /locus_tag="AC16G0_06930"
                     /inference="COORDINATES: protein motif:HMM:NF013348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP-loop family protein"
                     /protein_id="HIR5099391.1"
                     /translation="MSNQSLVKHLEQALREGGVVRSTRAVVAVSGGVDSMVLLDALVR
                     LQSRLGLRLHVVHVHHGLRGKAADQDAGFVVAEAVRRGVGVSLARLDPAERSRGISVQ
                     VWARNARYASLEAVRQRVKAAWVLTAHTLDDQ"
     gene            complement(642..3251)
                     /gene="clpB"
                     /locus_tag="AC16G0_06935"
     CDS             complement(642..3251)
                     /gene="clpB"
                     /locus_tag="AC16G0_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HIR5099392.1"
                     /translation="MRFDQFTLKAQEVIQTAQKNAEEMQHQAVDVEHLLLAMVEQTEG
                     ILTPILQKVGTSPRQIAASLRQELGKLPKVYGPSQAYITPRLNEVFKRAQEEAGRLKD
                     EYIGAEHLLLGVLGETSGVATQVLKSNGVTKDAVYSALQTVRGGQRITDPNPEDKYQA
                     LQRFSRDLTDLAQKGKLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVEG
                     LAERIVRGDVPESLKEKRVVALDLGSLVAGTKYRGEFEDRLKALLKEVTEASGKIILF
                     IDELHTLVGAGAAEGAVDASNMLKPALARGELRCVGATTLDEYRKRIEKDAALERRFQ
                     PIVVRQPSVEDTISILRGLRERYEVHHGVRIKDAALVAAATFSNRYITDRFLPDKAID
                     LVDEAASMLRMEIDSLPTELDEVERKLRQFGIEREAMKRETDPDTLERLERLEVEVAE
                     LEKRRSELRGRWEREKTSIQRIRAHKEQIEAARLEAEKAEREGNLGRVAEIRYGTMTG
                     LQKELKGEQAALGALEGHERMLKEEVDEEDIAKVVSRWTGIPVSKMLQTEAQKLLHME
                     DRLRERVVGQAEAIIAVSNAIRRARAGLQDENRPLGSFLFLGPTGVGKTELARALAEF
                     LFDDERALVRLDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRRPYTVILFDEM
                     EKAHADVFNILLQVLDEGRLTDGQGRTVDFKNSIIIMTSNLGSQYIREDEDEAVTREK
                     VTELLKTAFRPEFLNRVDETVIFHRLALPQIKQIVAIQLKYLQRRLGARKLELTVTER
                     AKEYLAREGFDPVYGARPLKRAIQRLVQDPLAQRLLEGDFQEGDRITVDLAGGEIVLQ
                     REETASVPGVRES"
     gene            complement(3688..>4117)
                     /locus_tag="AC16G0_06940"
     CDS             complement(3688..>4117)
                     /locus_tag="AC16G0_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007744196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099393.1"
                     /translation="TVGENLEVGRRPPRTGGSAWTAERVFDLFPALRPLEQRRGGSLS
                     GGEQQMLTIARTLMGQPSLLLLDEPSEGLAPRLVQAMTEQLLQLKREGTSILMSEQNL
                     HFAAQISDRAYILEKGRIQYQGSMNDLQADKEVRRRFLMV"
BASE COUNT          694 a         1431 c         1178 g          814 t
ORIGIN      
        1 ctgatcgtcc aaggtgtgcg ccgtcaggac ccaggcggcc ttcacccgct ggcggaccgc
       61 ctccagtgac gcatagcgcg cgttgcgcgc ccagacctga acggagatgc cccgagaccg
      121 ctccgccggg tccagccgcg cgaggctgac tcccactccg cgccggaccg cctccgccac
      181 cacgaagccc gcgtcctggt ccgccgcctt gcctcgcagc ccatgatgga cgtgcaccac
      241 gtggagtcgg agacccaggc gggattgcag gcgaaccaag gcgtccagga ggaccatgga
      301 atccacgccc cccgacaccg ccaccaccgc ccgcgtgctg cgaaccactc cgccctctcg
      361 gagggcctgc tccagatgct tcaccagact ctgattagac atgcctatcg tctaggagag
      421 agtttcactt ctgccgcgag ctcctgtgaa tccgtccgcg aaatctgcgg ccagaatacc
      481 tgctgtgttt ctcagatcga gattactcaa gcgcctccgc atcgtcaacc caattcccct
      541 gggggcagcc tgctcgaaca cacacgcccc tccggcgtgc agggccggag gggcgtgtgc
      601 cacgtggcca ttcacaagac aaggcgcgcg gtcggggacg tctagctctc ccgaactccc
      661 gggacggacg ccgtctcttc gcgctggagc acgatctccc cgcctgcaag gtcgaccgtg
      721 atacgatccc cctcctggaa gtctccctcc aagaggcgct gggccaatgg atcctggacc
      781 agacgctgga tcgcgcgctt gagcggtcgc gccccgtaga ccggatcgaa gccctcgcgg
      841 gccaggtact ccttggctcg ctcggtgacg gtcagctcca gcttcctcgc gcccagtctg
      901 cgctggaggt acttgagctg gatggcgacg atctgcttga tctgcggcag ggccagccgg
      961 tggaagatca ccgtctcgtc cacccgattc aggaactccg gccggaaggc cgtcttcaag
     1021 agctcggtga ccttctctct ggtcacggcc tcgtcctcgt cttcccggat gtactggctg
     1081 cccaggttcg aggtcatgat gatgatcgaa ttcttgaagt ccacggtccg cccctgtccg
     1141 tccgtcaggc gcccctcgtc cagcacctgg agcaggatgt tgaagacatc ggcgtgggcc
     1201 ttctccatct catcgaacag gatgacggtg tagggccggc ggcggacggc ctcggtgaga
     1261 taccctccct cttcgtaccc cacgtagcca gggggcgccc cgatgaggcg ggccaccgag
     1321 tgcttctcca tgaactcgga catgtccagc ctgaccaggg cccgctcgtc atcgaagaga
     1381 aactccgcca gcgcccgggc cagctccgtc ttgcccacgc ccgtgggtcc gaggaacagg
     1441 aaggagccca gcggccggtt ctcgtcctgg aggcctgccc gggccctgcg aatggcgttg
     1501 gagacggcga tgatggcctc cgcctggccc accacgcgct cgcgcagacg atcctccatg
     1561 tgcaggagct tctgggcctc tgtctggagc atcttggaca cgggaatacc ggtccagcgg
     1621 gacaccacct tggcgatatc ctcctcgtcc acctcttcct tgagcatccg ctcgtgcccc
     1681 tcgagggcgc ccagcgccgc ctgctccccc ttcagctctt tctgtagccc ggtcatcgtt
     1741 ccatagcgga tctcggcaac ccggcccagg ttcccttccc gctccgcctt ctctgcctcc
     1801 agcctggctg cctcgatctg ctccttgtga gcccggatgc gctggatgct ggtcttctcc
     1861 cgctcccagc gacctcgcag ctcgctgcgg cgcttctcca gctctgccac ctcgacttcc
     1921 agccgctcca agcgctccag ggtgtcggga tcggtctccc gcttcatcgc ctctcgctcg
     1981 atgccgaatt ggcgaagctt ccgctccacc tcgtccagct ccgtcggcag gctgtcgatc
     2041 tccatgcgaa gcatgctggc cgcctcgtcc acgaggtcga tggccttgtc gggcagaaag
     2101 cggtcggtga tgtaccggtt ggagaacgtc gccgcggcga ccaaggcggc gtccttgatc
     2161 cggacaccat ggtggacctc gtagcgctcc cgcagccccc gcaggatgga gatggtgtcc
     2221 tccacgctgg gctggcgcac cacgatgggc tggaagcgcc gctccagggc ggcatccttc
     2281 tcgatgcgct ttcgatactc atccagcgtg gtggcaccca cgcagcggag ctccccccgc
     2341 gccagggcgg gcttcagcat gttgctggcg tccacggccc cctcggccgc gcccgcgccc
     2401 accagcgtgt gcagctcgtc gatgaagagg atgatcttcc ctgaggcctc cgtgacttcc
     2461 ttgagcagcg ccttcagacg gtcctcgaac tccccgcggt actttgtccc ggccaccagg
     2521 gagcccaggt ccagggccac gacgcgcttc tccttgagcg actccgggac gtccccgcgg
     2581 actatgcgct cggccaaacc ctccacgatg gccgtctttc cgacgccggg ctctccgatc
     2641 aggaccgggt tgttcttggt ccggcgagag agcacctgga tcaccctccg gatttcctcg
     2701 tcacggccaa taacgggatc tagtttcccc ttctgggcca gatccgtgag atcccggctg
     2761 aatcgctgga gcgcctggta cttgtcctcg gggttcgggt cggtgatccg ctgaccgcct
     2821 cgcacggttt ggagggccga atagaccgca tccttcgtca ccccattgga cttgagaacc
     2881 tgagtggcga cccccgaggt ctcacccagg acgccaagaa gcagatgctc ggcgccgatg
     2941 tactcatcct tgagtcgacc cgcttcctcc tgggctcgct tgaagacctc gttcagacga
     3001 ggggtgatgt acgcctggga tgggccgtaa accttgggta gcttccccag ctcctgacga
     3061 agcgaggcgg cgatctgtct cgggctggtc cctactttct gcagaatggg tgtgaggatg
     3121 ccctctgttt gctccaccat ggcgaggagc agatgctcca cgtccacggc ctggtgctgc
     3181 atctcttccg cattcttctg ggcggtctgg atgacttcct gcgccttcaa cgtaaactga
     3241 tcgaaacgca cgtttctccc tccagctaaa gcgatttact tatttatacg ccatagaata
     3301 taaaacctga gccttactct gtcaatataa acttatacat aattaaatta agtacttgtg
     3361 atcatatctt gactcagaca atcgagtctt caagctggtc gccgaacagg ttcaatcgaa
     3421 taagctggtg ggaataatac tgatcgaagg aaaatggcgt agccacatgg cgctcggatc
     3481 tggtaattca ggaaagaaga tgttatcgct cctgacattc aagggctggc gacgaaccgg
     3541 cccagcgccc ggagcgcctc tctcttgacg gccttgcccc agcacagcac actttcctaa
     3601 tcgccaaccc agcgtgagcc tttactccgt tcgcgatgga cgccatatgc aggggcctca
     3661 tactcccaac gggtagactg caggatctca gaccatcaga aacctgcgtc gaacctcctt
     3721 gtctgcctgt agatcgttca tactcccctg gtactgaatc cgtcccttct ccaggatgta
     3781 cgcacggtcg ctgatctgcg cggcaaagtg gagattctgt tccgacatca ggatcgatgt
     3841 gccctctcgc ttcagttgga gaagctgctc ggtcatcgcc tgtactaacc gcggggccaa
     3901 cccctccgac ggctcgtcca ggagcaagag cgacggttga cccatgaggg tccgggcgat
     3961 cgtcagcatc tgctgctccc cgccactcag gctgcctccc cggcgctgtt ccagcggacg
     4021 cagggccgga aagaggtcga agacccgctc cgccgtccag gcgctgcccc ccgtcctggg
     4081 cgggcgtcgc cccacctcca ggttctcacc gactgtc
//
LOCUS       DBNGZC010000390         1751 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1499942, whole genome shotgun sequence.
ACCESSION   DBNGZC010000390 DBNGZC010000000
VERSION     DBNGZC010000390.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1499942"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="AC16G0_06945"
     CDS             complement(<1..436)
                     /locus_tag="AC16G0_06945"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5099394.1"
                     /translation="MTRCHGPVCVLHVGQLDVFAGEIVCLVGPTGSGKTTLLRLLSGL
                     EPTASGLVRYAAPLGSQSVPPRITMVFQHPLLLSGSVRYNVEYGLRLRGLARKGQEVD
                     PLLDRLNLIPLASRSIRTLSGGQRQLVALARALVLEPEVLLLD"
     gene            complement(466..1086)
                     /locus_tag="AC16G0_06950"
     CDS             complement(466..1086)
                     /locus_tag="AC16G0_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099395.1"
                     /translation="MWRSLHVSGVAVLVSSLVGIPIGTWLGLTRSRVRAILAVMIQTG
                     MALPPVVVELCLYLLLSRSGSMGTWGWLFTLKAMILAQIILTLPFILGITMVSVAAVP
                     PELFLQLRSLGASRWQARWTVLREVRAGVALAVATALGRSLSEVGAVLMVGGNIEGHT
                     RVLTTAIVLETSRGRFALALALGGVLLALALVANIAIIRLHGRPVP"
     gene            complement(1240..>1751)
                     /locus_tag="AC16G0_06955"
     CDS             complement(1240..>1751)
                     /locus_tag="AC16G0_06955"
                     /inference="COORDINATES: protein motif:HMM:NF019231.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1559 family PulG-like putative transporter"
                     /protein_id="HIR5099396.1"
                     /translation="TPAPMLSNLAQSSYVGVLGGGNKNNPDPLCGCYEWQPFNGVFHR
                     NSGIRIADITDGTSNTVGIGERDNGFVTSSWVGVLPGAEVIYNPDKGLGCTNWRPPIT
                     AVLAHSRQYTVNSPNASPAAFHTNHPGGGNFLFMDGSVRFIKDSVSLDTMRALCTRAY
                     GEVVSSDAY"
BASE COUNT          338 a          573 c          572 g          268 t
ORIGIN      
        1 cgtcgagcag gaggacctcc ggctccagca cgagcgcccg agccagggcc acaagctgcc
       61 gttgaccacc ggagagcgtg cggatcgagc gtgacgccag gggaatcagg ttgagccgat
      121 cgaggagggg gtccacctcc tggcccttcc gggccagtcc ccgcagtcgc aacccatact
      181 cgacgttgta tcggaccgat cccgagagga gcagcgggtg ttgaaacacc atcgtgatcc
      241 ggggcggcac cgactgcgaa ccgagtggtg ccgcgtagcg gaccaagccg ctcgccgtgg
      301 gttccaggcc cgagagcaac cgcagcagcg tcgtcttgcc ggaccctgtc gggccgacaa
      361 gacagacgat ctccccagcg aacacgtcga gctgtccgac gtgcagcaca cacacggggc
      421 catggcatcg cgtcacatcc ctcagctgga ggagcggtat cccgttcatg gtaccggtcg
      481 cccgtgcaag cgaatgatcg cgatgttggc gaccagtgcc agagccagca ggacgccccc
      541 caaggcgagg gcgagagcga agcgacctcg gctggtttcg aggacaatcg ccgtcgtcag
      601 gacccgggta tgcccctcga tgttgccgcc gaccatcagc acggcaccca cctccgagag
      661 actccggccg agggcggtcg cgacggcgag ggccaccccc gcccgtacct cacgcaacac
      721 ggtccagcgc gcctgccagc ggctcgcccc gaggctgcgg agctgaagga agagttcggg
      781 tggcacggcc gcgaccgaga ccatcgtgat tcccaggatg aaggggagcg tgagaatgat
      841 ctgggccagg atcatcgcct tgagcgtgaa aagccagccc caggttccca tggatccgct
      901 ccgagaaagc agcaggtaga gacacagctc cacgaccacc ggtggcaatg ccatcccggt
      961 ctggatcatc acggccagga tggcccgcac tcggctccga gtcagtccca gccaggtccc
     1021 gatgggaatg ccgacgagcg aactgaccag gacggcgacc ccggaaacat gcagcgaccg
     1081 ccacacggtc gatcccagtt cagaggcgac atccatctca cgcctgacct tcctcgaaac
     1141 cgaccgctcg aacaaggctc ggggcgggag gagagtctgc taccgtttgc attctggtct
     1201 cttgcccgat cggttgttca cagccagggc cgcaaggctt caataggcgt cgctcgagac
     1261 cacctcgcca taggcacgag tgcagagggc tcgcatggtg tccaagctca ccgaatcctt
     1321 gatgaaccgg accgagccgt ccatgaagag aaagttgccc ccaccggggt gattcgtatg
     1381 gaaggcggcg gggctggcgt tcggggagtt cacggtgtac tgccggctgt gggccaggac
     1441 ggctgtgatc ggcggccgcc agttagtgca gccgagtcct ttgtccgggt tgtagatgac
     1501 ctccgcgccc ggaaggacgc cgacccaact gctcgtgacg aagccgttgt cccgctcccc
     1561 gatccccacg gtgttcgagg tcccatcggt gatgtcggcg atgcggatcc cgctgttgcg
     1621 gtggaaaacc ccgttgaagg gctgccattc gtaacagccg cagagcgggt ccggattgtt
     1681 cttgttgccc cctcccagga cgcccacgta gctggactgg gccagattgg agagcatcgg
     1741 ggccggcgta t
//
LOCUS       DBNGZC010000391         4038 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1596205, whole genome shotgun sequence.
ACCESSION   DBNGZC010000391 DBNGZC010000000
VERSION     DBNGZC010000391.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1596205"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="AC16G0_06960"
     CDS             <1..608
                     /locus_tag="AC16G0_06960"
                     /inference="COORDINATES: protein motif:HMM:NF019758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099397.1"
                     /translation="RELKESYWDPFLRVVEVLRHFGCLEDSRLTPTGRLIASLRHDNE
                     LLVARVAFSGLLEGLQPPAAAALLSCLVEEPRETETLFARQLFKRLPHLRRRVQALEG
                     LAAEVLRVQQARRVDRPVSMHTTYLAASYEWTNGEDDWVRLIRNCFGGHEGDLIRAFR
                     RLIDLCRQLIDSPELPEGLRVSLLKAVEMLDRGIVLESALI"
     gene            808..1128
                     /locus_tag="AC16G0_06965"
     CDS             808..1128
                     /locus_tag="AC16G0_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099398.1"
                     /translation="MIAFRDLVAAQLASRHPSTEGWIVQRDHLLDSGAEVHFLASRGR
                     ESILVHCKEEAGNVLFEHVDHAAGLATEAGAGSAVLYVPIGSFTPPVILAYAAKRRIR
                     IEAV"
     gene            1223..3403
                     /locus_tag="AC16G0_06970"
     CDS             1223..3403
                     /locus_tag="AC16G0_06970"
                     /inference="COORDINATES: protein motif:HMM:NF016348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HIR5099399.1"
                     /translation="MRPHAAAIATFLLAVFFATSGRAAEGLGRDLERLQRLRDQVEVN
                     ADHLEYHEAERKLVASGNVRLVMGERSLFADEASVDLDDQVLVATGHVILMEGLNRLE
                     GDRIEYNYRTNLGVVTAGRGFLAPGISFSGSEIRREAERQYHITGGRFTTCRVCEPEP
                     QTVDWEFRTQDATIYQDEWIVARNTSVWLKGIPALFSPIAALPIGPRRTGFLIPRLGY
                     GNRDGFTIRQPFFWAISPSQDATFTTTYKSRSGVDVLGEYRYILSENSRGELAARYFY
                     DRDTTPHNRAEFKWLHDQVLNPTWSFKADVRTQTQPSLNSQTEDSTVAERTQRVLDSK
                     AFLTQATSRYMLLGLLEVTQDLSNVVQTHTSRLPDLRFQWLPNRVFKLPVVPEAEASV
                     VYLERDHGVDTGRFDLRPGVHVPLPLTSWLMATTSAGLRETAYTATTLPDRSGNRIVA
                     ELGERLTSRFARRFEEPGFGLLRLTHVVEPSLLYQYVPWTDQRALPQFDRTDFISPQN
                     RLTYQLMNRLVARWREASGETRTHEVATLDVAQSWNLQPRTREFSDVYLTGLTPERVD
                     QAVSNAVSLGDGFSRAEERTLSNLVFNGSISPLPAVALVGTLAFNTETRQTDAINSGL
                     QLRFPDLLNLEVGSSYVRGQTVNGLVGKIQWYITKTVMVDYLTRYDSPTNTFLEHTAS
                     LRYSTCCWEVALRLSHKARGVNQKAENSAHVTFDLKVPTSPASR"
     gene            3576..>4038
                     /locus_tag="AC16G0_06975"
     CDS             3576..>4038
                     /locus_tag="AC16G0_06975"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5099400.1"
                     /translation="MRFDAARAPRLVAWEVTRACDLACVHCRAVAQPSADPRQLSTQE
                     AFRLVDDIAAFREPVILILTGGDPLKRPDIFEVAARASQAGLRVVMSPSGTHVTPASV
                     RELKQVGVQRISVSLDGSTPELHDAFRQVPGAFAAATQSLAYAREGGLPFQI"
BASE COUNT          666 a         1354 c         1286 g          732 t
ORIGIN      
        1 tgcgggagct gaaggagtcg tactgggacc cgttcctccg ggtcgtggag gtcctgcgac
       61 acttcggctg cctggaggac tccaggctca ccccgacggg ccgcctgatc gcgtcgctcc
      121 ggcacgacaa cgagttgctc gtggcccgcg tggccttctc gggcctcctg gagggtttgc
      181 agccgcctgc ggccgcggcg ctgctctcct gtctggtgga agagccccgg gaaaccgaga
      241 cgctcttcgc ccggcagctg ttcaagcggc ttccgcatct tcgccgcagg gtccaggcgc
      301 tggagggcct ggccgccgag gtcctgcggg tgcaacaggc ccggcgggtg gaccggccgg
      361 tctcgatgca caccacctat ctggccgcca gctatgaatg gaccaacggc gaggacgact
      421 gggtccggct gatccgaaac tgcttcggcg gccacgaggg agacctcatc cgcgccttcc
      481 gccgcctgat cgacctctgc cgtcaactga tcgacagccc ggagcttccc gagggccttc
      541 gcgtctccct tctcaaggca gtggagatgc tggaccgcgg catcgtccta gagtccgcct
      601 tgatctgatc cggcaatggt accacggcgg ttcctggggt tgtgacgcgg aggaccatct
      661 gaatctccag ggcacccact gagtcccaag ctcccctgca tcttgcgtcg cgcttctctt
      721 tcccttgcct tgacatccat cttcgcctcg tgttacacag ccgacggtgg gtcggggcgc
      781 accgcagggg aaacggctgc catggcgatg attgcctttc gagatctggt cgctgctcag
      841 cttgcgtccc ggcacccaag caccgagggc tggattgtcc agcgggatca cttgctggac
      901 agtggcgcgg aggtgcattt cctggcaagc cgtggccggg aaagcatcct ggtccattgc
      961 aaggaagagg cgggcaacgt tctgttcgag cacgtggacc acgcggccgg cctggccaca
     1021 gaggcggggg cgggctccgc cgtcctgtat gttcccatcg gctctttcac cccgccagtc
     1081 atcctggcgt acgcggccaa gcgccgcatt aggatcgaag ctgtctgatg cgggccggcc
     1141 tgttccgctg gtgacttctc ccttgctcgg gcttccggcc tctggtataa ggcggcgtcc
     1201 cccacccctg tgcggagccc gcatgcgacc ccacgccgct gccatcgcca ctttcctatt
     1261 ggccgtcttc ttcgcaacct cgggccgcgc cgccgagggg ctcggccggg acctggagcg
     1321 tctccagcgg ttgcgggacc aggtggaggt gaacgccgat cacctggagt accacgaggc
     1381 cgagcgcaag ctggtggcca gcggcaacgt ccgcctggtc atgggggagc gttcgctgtt
     1441 cgccgacgag gcatcggtgg atctggacga ccaggtgctg gtggcgacgg gccacgtgat
     1501 cctcatggag gggctgaacc gtctggaggg ggaccggatc gagtacaact accgtacgaa
     1561 cctgggcgtc gtcaccgccg gtcggggatt cctggcgccc gggatcagct tcagcggctc
     1621 ggagatccgg cgcgaggcgg aacggcagta ccatatcacg gggggacgct tcaccacgtg
     1681 tcgcgtctgc gagcccgagc cccagacggt agactgggag tttcgaaccc aggacgccac
     1741 catctaccag gacgagtgga tcgtggcccg gaacacctcc gtctggctca agggcatccc
     1801 cgcgctgttc tccccgattg ccgcactccc catcgggccg aggcggacgg gctttctcat
     1861 tcctcgcctg ggttatggca accgcgacgg cttcaccata cggcagccgt tcttctgggc
     1921 catctcgccg tcgcaggatg cgaccttcac caccacgtac aaatccagaa gcggcgtcga
     1981 cgtgctgggg gaatatcggt acatcctctc ggagaattcg cgaggcgagc tggccgcccg
     2041 ctacttctat gaccgggaca cgacccctca taaccgggcg gagttcaaat ggcttcacga
     2101 tcaggtcctg aaccccacct ggtcgttcaa ggccgatgtg cgaacccaga cgcagccatc
     2161 gctcaacagc cagaccgaag attccacggt ggccgaacgc acccagcgcg tcttggattc
     2221 caaagccttc ctgacccagg caacgtcacg ctacatgctc ctgggcctct tggaggtcac
     2281 acaggacctc tccaacgtgg tccagacaca cacctcacgc cttccggacc tgcgattcca
     2341 gtggctgccg aatcgagtct tcaaattgcc cgtggtgccg gaggccgagg cgtccgtcgt
     2401 ctacctggag cgtgaccatg gagtcgacac gggccgtttt gatcttcgcc cgggcgtgca
     2461 cgtccccctg cccctgacct cctggctcat ggccacgact tcggcggggc tccgcgagac
     2521 ggcctacacg gcgacgactc tgcccgaccg gagcgggaat cggatcgtgg ccgagttggg
     2581 cgagagactg accagccgct tcgcccgccg gttcgaagag ccgggctttg gtctgctgcg
     2641 cttgacccac gtcgtcgagc cgagcctcct ctaccagtat gtcccgtgga ccgaccagcg
     2701 ggcgctcccg cagttcgacc gcactgactt catcagcccc cagaaccggc tgacctatca
     2761 actcatgaat cggctggtgg cgcgatggcg cgaggcgagc ggggagaccc ggactcacga
     2821 ggtggccacc ctggatgtcg cccagagctg gaacctgcag ccacgcaccc gggagttctc
     2881 ggacgtgtac ctcaccggtc tcacgccgga gcgggtggat caggccgtgt cgaacgccgt
     2941 gtccctgggg gacggcttct cccgggcgga ggagcggaca ctgtccaacc tggtcttcaa
     3001 cgggtcgatc agcccgctgc ctgccgtggc cctggtgggg acattggcct tcaataccga
     3061 aacgcggcag accgacgcca tcaactctgg tctccagctt cgcttcccag acctcctcaa
     3121 cctggaggtc ggctccagct acgtccgggg tcagacggtg aatggcctgg tcggaaagat
     3181 tcagtggtat atcaccaaga ccgtcatggt cgactacctg actcgctacg attcgcccac
     3241 caacacattc ctggagcata ccgcctcgct ccggtacagc acatgttgct gggaggtcgc
     3301 ccttcgatta agtcataagg cccggggtgt gaaccagaag gccgagaaca gcgctcacgt
     3361 caccttcgac ctgaaagtcc ccacgtcgcc ggcatcacgg tagatcggaa gccggctggg
     3421 cctgggcacg cttccccggt ccagttgggc ctgacgaatg gcttggtctt gggccatcgg
     3481 gccccgattt gaacaatgct cagataggag ttgaccgacc ggtacggccc tatttagaat
     3541 accagaagtt caggcttgtt caggggaggg gattcatgcg attcgatgcc gcccgcgcgc
     3601 cgcgtttggt ggcctgggaa gtgacccggg cctgcgatct ggcctgcgtc cactgccgcg
     3661 ccgtggctca acccagtgcc gatccccggc agctctccac ccaagaagcc ttccgcctcg
     3721 tggacgatat cgcggcgttc cgggagccag tgatcctcat cctgacgggt ggcgatccgt
     3781 tgaagcgtcc ggacatcttt gaggtcgcgg cgcgtgcgtc ccaggcaggt ctcagggtcg
     3841 tgatgtcgcc cagcggcacc cacgtgaccc cggcgagcgt gagagagttg aagcaggtgg
     3901 gggtccagcg catctccgtc agcctggacg gaagcacccc ggagctgcac gatgcgttcc
     3961 gccaggtgcc tggggccttc gcggcggcca cccagtcgct ggcatacgcc cgcgaggggg
     4021 ggctgccatt ccagatca
//
LOCUS       DBNGZC010000392         2914 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1010187, whole genome shotgun sequence.
ACCESSION   DBNGZC010000392 DBNGZC010000000
VERSION     DBNGZC010000392.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1010187"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..757)
                     /locus_tag="AC16G0_06980"
     CDS             complement(71..757)
                     /locus_tag="AC16G0_06980"
                     /inference="COORDINATES: protein motif:HMM:NF025605.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5099401.1"
                     /translation="MPVAKPRPRVPRWRRLPDARPQQILEAAFRVFGTRGLHQATLDD
                     VAKAAGITKGTIYLYFPSKAALFTAMLKTRVNAVMPPVELPLDGRDTQETRVRLLGLG
                     QRLYRFFRTPAFLAMYRSVVSEASLFPEAAATLYREGILPANQRLAAIIRRGIVTGEF
                     RDVDPLIAARAFVGMFQIFAVSQGLLGGQRVYPMSEAHVVQVVTDLFFRGLLAPRQGE
                     ARRRSVRGLQ"
     gene            complement(1446..2282)
                     /locus_tag="AC16G0_06985"
     CDS             complement(1446..2282)
                     /locus_tag="AC16G0_06985"
                     /inference="COORDINATES: protein motif:HMM:NF015213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HIR5099402.1"
                     /translation="MDILSRLFESLIVTLREGVEVALVVGILLAYLRRTGRGAYARQV
                     FLGLGAAILASLLGAVLIYHYGLNPDNPLAEGITMFVAAGLVTSLVVWMWKTGRTVRR
                     RLEQRLDALVGPADTSAVTHRAALGVFGFTFVMMFREGIETVLFLSALSGTVGGSPLY
                     NLLGGSLGLLSAALFGVLLVRGSLHLNLGRFFRVTGVVLLILVAKLVAGGLHEFFEVS
                     LLPSTPLLEEVVEFFTNGTTSLIILFLLMALPGACMLWDWWKHTSNEQVSLPPQPNGA
                     SG"
     gene            complement(2383..>2914)
                     /locus_tag="AC16G0_06990"
     CDS             complement(2383..>2914)
                     /locus_tag="AC16G0_06990"
                     /inference="COORDINATES: protein motif:HMM:NF019292.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HIR5099403.1"
                     /translation="PGRGTGLVMEMFPFRRPGALAILKKTWYRFHDFIVVATPIVLGG
                     SLILGGLHETGLLWALATPLRPIMEGWLGIPAVAGLTLIVAVLRKELALQLLVTLAMA
                     QYGPQASTLLTFMRPDQIVVYALVNTIYIPCVATIAILNRELGWRWAAMISTFTVLLA
                     LLVGGAAHGIFMMLHS"
BASE COUNT          567 a          906 c          990 g          451 t
ORIGIN      
        1 caaccgccca gggtgagcgc agcgaggacc gcggcgatgc cgaggatcag aacggggcgg
       61 gcttggcgct tcattggagg cctcgcacgg agcggcgccg ggcctcgccc tggcgggggg
      121 ccagcaggcc gcggaagaag agatcggtga ccacttgcac gacgtgggct tcggacatgg
      181 ggtagacccg ctgacccccc aggagtccct gggagactgc gaagatctgg aacatgccca
      241 cgaaggcccg ggcggcaatc aaggggtcca cgtcccggaa ttcccccgta acaatgcccc
      301 tccggatgat ggccgccagc cgctggttgg cgggcaagat gccttcccgg tagagcgtgg
      361 cggcggcttc ggggaacagg gaagcctcgc tcaccacgct gcgatacatc gccaggaacg
      421 ccggcgtccg gaagaaacgg tacaggcgct ggcccaggcc caagagacga acccgcgtct
      481 cctgcgtatc gcgcccgtcc aggggcaact ccacgggggg catgacggcg ttcacccggg
      541 tcttcagcat ggcggtgaag agggcagcct tgctggggaa gtacaggtag atggttccct
      601 tggtgatccc cgcggccttg gcgacatcat ccagggtcgc ctggtggagc cctcgggtgc
      661 cgaagacccg gaaggcggcc tccaggatct gctgcggcct ggcatcgggg aggcgacgcc
      721 agcgcgggac gcggggtctg ggcttggcaa cgggcatggg cactctccaa atatctgacc
      781 ggttagttag taacattacc ttccgcagcc cgccctgtca agcgatagtt cgtcgggaga
      841 cttacccgcc ggaggcggcg gggccgttgg gtgacctgcc acttccccga ctcaggcagc
      901 cggaagtcgc gaggtggcgg ttccgaaccc tgcgcatggg tgggcccggc ctccggaccc
      961 tgaatctggc gtggttagca gcccaggccc gttgctctcc gggaattccc gagaaatgtg
     1021 tgtgattgcg gtcgcaggag gccagccctc cccgccctat gttataggcg ttggcaagcg
     1081 aggggacgga gacttgtgcg cattcgcttc actaggattg ggcataagct ctagaggggg
     1141 tgaggcagat ggaagggatg ttccttcagt gggttccggt gagaggatcc ttagttgagg
     1201 gggtattgct cgtggcgatg gtggtcctgg ctgtggtgta cgggcggttc caccagaagc
     1261 cttctcaaac tgcccagggc cattagccgg ccgcccggtc gcgtaccgcc ccgtaccaga
     1321 tccttccagg cggtcggaga tcgtgccacg actccgaccg cctcgtcatc tcgcccaggg
     1381 tatccccgga caccggaatg ctcaggcaag aatcgtccct gacgggttct gccggccggc
     1441 tcagcctagc cgctcgcgcc gttcggctgg ggagggagcg aaacctgctc gttcgaggtg
     1501 tgcttccacc aatcccagag catgcaggcg cctggcaggg ccatgagcag gaataggatg
     1561 atgagggagg tggtcccgtt cgtgaagaac tcgacaactt cctccaggag cggcgtactg
     1621 gggaggagac tgacctcgaa gaattcgtgg agaccgcccg cgacgagctt ggccaccaag
     1681 atcagaagga cgacaccggt cacgcggaag aaacggccca ggttgaggtg aaggcttccc
     1741 cgcacgagca gaacgccgaa cagggcggcc gacaggagac ccaggctgcc ccccaggagg
     1801 ttgtacaggg ggctgccccc gacggtgccg gagagggcgg acaggaagag aaccgtctcg
     1861 atgccttcgc gaaacatcat gacgaaggtg aagccgaaga cgcccagggc cgcgcggtgc
     1921 gttacggcgg aggtatccgc cgggcccacc agggcatcca gtcgttgctc cagccttcgc
     1981 ctgacggtcc ggcccgtctt ccacatccac accaccaggg aggtgaccag cccggctgcc
     2041 acgaacatgg taattccttc cgccaaggga ttgtccggat tcagcccgta gtggtaaatg
     2101 agaacggcac ccaggaggct cgccaggatg gcggcgccaa gccccaggaa gacctggcgc
     2161 gcataggcgc cgcggccggt ccggcggaga taggccagga ggatgcccac caccagggca
     2221 acctcgactc cctcgcgaag tgtaacgatc aggctctcga acaagcggct cagaatatcc
     2281 atgacccccc ccaaagactt ctgactatgt gaggaccggt gcgggacccg attgcggcgc
     2341 tcacggaatg ccggcaaaac cagggccgcc ttccattccc ggtcacgaat ggagcatcat
     2401 gaagatcccg tgtgcggccc cgccgaccag cagagccagg agcaccgtga acgtggaaat
     2461 catcgccgcc caccgccatc ccagctcccg attcaggatg gcaatggtag cgacgcaggg
     2521 gatgtagatc gtgttgacca gcgcgtacac cacgatctga tcgggtcgca tgaaggtgag
     2581 cagggtgctg gcctgcgggc cgtattgcgc catggccagc gtcaccagga gttgcaatgc
     2641 cagctccttc cggaggactg ctacgatcag ggtaagaccc gccacggcgg gaatccccaa
     2701 ccatccctcc atgatcgggc ggagaggtgt ggccagggcc cagagaaggc ccgtttcgtg
     2761 gaggccgccc aggatgaggc tgccgcccaa gacgatgggc gtggccacga cgatgaaatc
     2821 atggaagcga taccaggtct tcttgaggat cgccaaggcc ccggggcggc gaaatgggaa
     2881 catctccatc accagtccgg tcccgcgtcc cggc
//
LOCUS       DBNGZC010000393         1025 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2576340, whole genome shotgun sequence.
ACCESSION   DBNGZC010000393 DBNGZC010000000
VERSION     DBNGZC010000393.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2576340"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="AC16G0_06995"
     CDS             complement(<1..182)
                     /locus_tag="AC16G0_06995"
                     /inference="COORDINATES: protein motif:HMM:NF012415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIR5099404.1"
                     /translation="MQIVNLTNQQRQQNGLPPLQVNTKLVQAAQIHSRDMAQFNIMDH
                     NLPGAAQPTLQDRINYV"
BASE COUNT          178 a          266 c          391 g          190 t
ORIGIN      
        1 acgtaattga tgcggtcctg cagcgtcggc tgggcggcgc cggggaggtt gtggtccatg
       61 atattgaact gggccatatc gcgggagtgg atctgcgcgg cctgcaccag cttcgtgttg
      121 acctgcagag gcggaagccc gttctgctgg cgctgctggt tcgtcaggtt gacgatctgc
      181 atctccaggg cggattccgt cccggaaacg gggttgaccg tgggtggcgg aatcacccgt
      241 gggggtgcga cgggggccgg ggcccgggag ggggactttc tcagcgaggg cggacaagcg
      301 cggacagttt cagcagtgga cgctgaggca gtaggtggcg cggaactcct tctcttgcag
      361 aggcttgctt cggttctcgg gctgccttgg ctgtccaaag tgtccgcgct gtccactggt
      421 cctggcaccg tcagagagaa agtcccccgg gagggaaggt gccgtaggag gattcgtttg
      481 ggcagagcgg gggcccgagg gggcagcgac cgtgagacgg tccgtatcgg cagggatcgt
      541 ggggcgactc gtttgtgcgg aaccggagcc cgaggaggca gcgaccgtga gacggtccgt
      601 tgcgcgacca gccgctgctg ggccaggatc gggtggctct gcctgtggag attgaccgag
      661 acgtggttct gggcggcggc cagtgccttc aggtgggaga gggaagcggc gctggcaacc
      721 ggtgataggc cagccgatgg gagaacgcgg tcgtcgaggg catcaacact cggacggtac
      781 cgattccgcg cagatcgcct cgttacctgt gcatttgttg tagaaaagac gttcatggca
      841 ttttcatccc gttcacgtgc gggcgatata acacacattc ctcaattcaa ccctaggtca
      901 atttcaagaa atcgttgggg agtcggaggc ggtgggaggg aggggtgtgc gaaccgagca
      961 acatcgactc cgatcagcgg tcccaggggg cgagagcgcg aagcgaagcg cggccagccg
     1021 ctgcg
//
LOCUS       DBNGZC010000394         2086 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2380428, whole genome shotgun sequence.
ACCESSION   DBNGZC010000394 DBNGZC010000000
VERSION     DBNGZC010000394.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2380428"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="AC16G0_07000"
     CDS             <1..672
                     /locus_tag="AC16G0_07000"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR5099405.1"
                     /translation="AFCEYVMRPPLALHKIPDNVSLVDAVIVNQGSMVLHGCNRVGIL
                     PGESVAISGPGLSGLLALQVARAKGATKIIMIGRGDRLRVATELGADHVVDFTREDPA
                     RAVQALTDGLGADVGMECAGSPESLSPLTYAVRRGARVAVIGLNGKKQVPIITDRISL
                     DEVDLIGVRSGAGEYPTFISLMAAGKVSAKGLITHTFPLEEITQAMEAFRERRGGAIR
                     VAIEI"
     gene            759..1496
                     /locus_tag="AC16G0_07005"
     CDS             759..1496
                     /locus_tag="AC16G0_07005"
                     /inference="COORDINATES: protein motif:HMM:NF024634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR5099406.1"
                     /translation="MLINPEVCIGCENCAPHCPVEAIYATEQNTRKGTRIRAIDLDLC
                     VECGTCLRADVCNVEAIYQQPLEWPRVLRASFSNPLKPHGHTGLLGRGTEEVKTNEVT
                     HRYVRGQVGVAVEMGRPGIGTSLRDIDRLTRAMADHGVEFERNTPVTGLMSDPVRGTL
                     KEDVLDERVLSAIIEFKLPEARLSEILPALRQVAKTLDTVFSLGVISVLPPEGPDPVA
                     GRIKSLGFDLRPNGKVNLGLGRPLPKQ"
     gene            1545..>2086
                     /locus_tag="AC16G0_07010"
     CDS             1545..>2086
                     /locus_tag="AC16G0_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099407.1"
                     /translation="MTHMLHRTGEADSLREDYPMLAIRAKGFNDEGNDWRLRKILEIA
                     ARHPIVNFSDDKTGSMFTASLEEVLHSEQTDLPMVQMVFTSTEDMTRFMEDLKEVDLG
                     ISVVASGLLEEIFQCCRKTGLKPHSVHYSLGVHGRVEKLPSKETMEITTMCGHALVSK
                     NLVTKLVADIKAGKETGSTAA"
BASE COUNT          405 a          620 c          694 g          367 t
ORIGIN      
        1 gcattctgtg agtatgtgat gcgcccgccc ctggcgctcc acaagatccc ggataacgtc
       61 agcctcgtgg acgccgtgat tgtgaaccag gggtccatgg tgctccacgg ctgcaaccgg
      121 gtggggatcc tgccgggcga aagcgtggcc atctcgggcc cgggactctc aggtctcttg
      181 gcgctccagg tggcgcgggc caagggcgcc accaagatca tcatgatcgg tcggggtgac
      241 cggctgaggg tcgccaccga gttgggggcg gaccatgtgg tggacttcac ccgggaggat
      301 ccagctcggg ccgtccaggc actgacggat ggcctggggg ccgatgtggg aatggagtgc
      361 gcaggctccc ctgagtctct cagtcccctc acctacgccg tgaggagggg tgcccgcgtc
      421 gcggtgatcg gcctgaacgg caagaagcaa gtgcccatca tcacggatcg catctctctg
      481 gacgaggtag atctgatcgg agtgcggagc ggagcgggtg agtaccccac cttcatctcg
      541 ctaatggcgg cgggcaaggt ctccgctaag gggttgatca cccacacctt cccgctggaa
      601 gagatcaccc aggccatgga ggccttccgc gagcgccggg gcggggccat ccgtgtcgct
      661 atcgagatct gatcgcccat gggttgcgga ggccgaaggg aggagcggcc ctccctcgta
      721 tcgttgacat cggtgaacag gaggccagaa agcgatccat gctgatcaat ccggaagtct
      781 gcatcggatg cgagaactgc gcccctcact gccccgtaga ggcgatctac gccaccgagc
      841 agaatacgcg caaggggacg aggatccggg caatcgatct tgacctgtgc gtggaatgcg
      901 gcacctgcct ccgtgcggac gtctgcaatg ttgaggcgat ctaccagcaa cccctggagt
      961 ggccgcgggt gttgcgcgcc tccttctcga accccttgaa gccccacggg cacaccggcc
     1021 tcctgggacg cggcaccgag gaggtgaaga cgaacgaggt gacccatcgc tacgtgaggg
     1081 ggcaggtggg cgtggcggtg gagatggggc gccctggcat cggcacgagc ctgcgggaca
     1141 tcgaccggct cacgcgggca atggccgatc atggcgtcga gttcgagcgc aacacgccgg
     1201 tcaccgggct catgtccgat cccgttcggg ggactttgaa ggaagacgtt ctggatgaac
     1261 gggtcttgtc ggccatcata gaattcaagc tcccggaagc gcggctctcg gaaatcttgc
     1321 ctgctctaag gcaggtggca aagactctgg acacggtctt ctccctgggg gtcatcagtg
     1381 tccttccccc ggaggggcct gatcctgtgg ccgggaggat caaatcgctc ggtttcgacc
     1441 tgcggcccaa cgggaaagtc aatctcgggt tgggacggcc gctcccgaag caatgatcca
     1501 cgggtaaccg ctgagagcgg aagtctggtt tcggaggttc cggaatgacg cacatgctgc
     1561 atcgaaccgg agaggcagac agcctgcgcg aagattatcc gatgctcgcc atccgagcca
     1621 agggcttcaa tgacgaggga aacgactgga ggctgcggaa gatcctggag atcgccgccc
     1681 gccacccgat cgtgaacttc tccgacgaca agaccggctc gatgttcact gccagcctcg
     1741 aggaggtcct ccactccgag cagacggacc tccccatggt tcagatggtc ttcaccagca
     1801 cggaggacat gacccggttc atggaggatt tgaaagaagt ggacctgggc atctcggtgg
     1861 tcgcgtcggg gttgttagag gagatctttc aatgttgccg caagaccggc ctcaagccgc
     1921 actcggtgca ctactccttg ggcgttcacg gccgggttga gaagctcccc tccaaggaga
     1981 ccatggaaat caccacaatg tgcggtcatg ccctggtatc caagaacctg gtgaccaagc
     2041 ttgtggctga catcaaggca gggaaggaga ccggctccac cgcagc
//
LOCUS       DBNGZC010000395         2411 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1106529, whole genome shotgun sequence.
ACCESSION   DBNGZC010000395 DBNGZC010000000
VERSION     DBNGZC010000395.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1106529"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="AC16G0_07015"
     CDS             <1..1156
                     /locus_tag="AC16G0_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5099408.1"
                     /translation="REKRELFSRPYYAYTLQLMVRKNEERIRSIHDLSGKKVGTLAGA
                     VAHDMLQKIGGVEIRSYSAAWPYEDLALGRLDAVFLDTPIAAYYGKPDPRLKYVGPPE
                     GGGFYGIAIRKADWELKQVVDSVIENLLRTGDLKRIYEKWELWDEAQTRLTAAAAPSD
                     AGVQFRTESADAPLYSFLPLLLRGAGITIILSVVSMALAMAVGLLLAVTRFYKPPVVG
                     GLATAYVEIYRGTPLLIQLFIIYYGLPNIGIALSPLAAAIIGLGMNYAAYEAEVYRAG
                     IEAVPHGQVEAALSLGMPRSVTFQTIIFPQAFRISLPAVTNDFIALFKDSSIVSVIAM
                     VELTKTYGILAATTLRYFELGLVVAILYFGMSYPLSILARRIERRLRGAK"
     gene            complement(1215..>2411)
                     /locus_tag="AC16G0_07020"
     CDS             complement(1215..>2411)
                     /locus_tag="AC16G0_07020"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - obsolete cysteine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR5099409.1"
                     /translation="AKSVEEIRRDMPCLKDLVYLDSASVVPAPRPVIEAMQEYLCIHP
                     FNYGVGVWRRSLEAAERVNAARHIAAHAVGAAADAEIAFTKNTTEAINLVAQGLDWKP
                     GDEIVLTELEHQSNTIPWMRAAQEKGGRVRFVPADPDGLVDPNDVGRALTPATRLVAC
                     THVSNVFGTIQPVTEITRIAHAHGALAFVDAAQSAGRIPVRVDEIGCDFAAFCGRKAL
                     MGPQGTGFLWGRLELLQRLAPLATGSRAANVVDAEGFRLNQPPHKFEAGVLNTAGVIG
                     LGRAIQYVDEIGLDAILARIRELTAYMIEILQSTPKVIFHGSARLDRQAGIVMWSLEG
                     MEPDRAARELDRIGNVAVASGAQGSLLAIKPKGVTSIVRTSVHYCNTLEDLDALAAAL
                     RQVVAR"
BASE COUNT          406 a          797 c          765 g          443 t
ORIGIN      
        1 gcgcgagaaa cgggagctct tctcccggcc gtattacgcc tacacgctgc agctcatggt
       61 gcggaagaac gaggagcgca tccggagcat ccacgacctt tcgggaaaga aggtggggac
      121 gctcgcaggc gcggtcgccc acgacatgct ccagaagatc ggtggggtgg agatccggtc
      181 ctattccgca gcgtggccct atgaagatct ggccctgggg aggctggatg cggtctttct
      241 ggacacgccg atcgccgcct attacggcaa gccggatccg cgtctcaagt acgtcggccc
      301 accggaggga gggggtttct acgggatcgc catcaggaaa gcagattggg agctcaagca
      361 ggtcgttgac tcggtgatcg agaacctctt acgcacgggc gatctcaagc gtatctacga
      421 gaagtgggaa ctctgggacg aggcccaaac caggctgacc gccgccgcgg caccgtccga
      481 tgcaggcgtg cagttccgga cggaaagcgc cgacgcgccc ctctattcct tcctgccgct
      541 cctgctgcgc ggggccggaa tcaccatcat tctttccgtt gtctccatgg cgctggccat
      601 ggccgtgggc ttgctcctcg cggtgacgcg attctacaag cctcccgtcg tcggcgggct
      661 ggccaccgcc tatgtcgaga tctaccgcgg cacgccgctt ctgattcagc tcttcatcat
      721 ctactacggt ctgccgaaca tcgggatcgc gctgtcaccc ctggccgcag ccatcatcgg
      781 cctcggcatg aactacgccg catacgaggc ggaggtctat cgggcaggca tcgaggccgt
      841 accccacggc caggtggagg cagccttgtc cctgggcatg ccgcgctccg tgactttcca
      901 gacgatcatc ttcccccagg ccttccggat cagcctcccg gcggtgacga acgacttcat
      961 cgcgctcttc aaggattcct cgatcgtttc ggtgattgcc atggttgaac tcaccaagac
     1021 ctatggcatc ctggccgcca ccacgctccg ctatttcgag ctggggctcg tcgtggctat
     1081 cctgtacttc ggcatgagct atccgctctc gatcctggcg aggaggatcg agaggagatt
     1141 gaggggggcg aagtgatcga ggggagacag tctcagactg gatcgtcgcg cgcattcctt
     1201 gcaacgcgtc ttcgctatcg ggccacgacc tgccgcaggg ctgccgccag ggcatcgagg
     1261 tcttccaggg tattgcagta gtggaccgac gtcctgacga tggacgtcac ccccttaggc
     1321 ttgatcgcca aaagcgaccc ctgggcgccc gaggcgacgg caacgtttcc gatccggtcc
     1381 agctccctgg ccgcccgatc cggctccatc ccctccaggc tccacatcac gattccggcc
     1441 tgacggtcca gccgggcgct gccgtggaag atcaccttgg gcgtggactg caggatctcg
     1501 atcatgtagg cggtcaattc ccggatgcgg gcgaggatgg cgtcgaggcc gatctcgtcc
     1561 acgtactgga tggccctgcc cagcccgatg acccctgcgg tgttcagcac gccggcctcg
     1621 aacttgtgag gaggctggtt cagccggaag ccctcggcat ccaccacatt cgccgcccgg
     1681 ctgccggtcg cgaggggtgc gagtcgctgc agcagctcca ggcgacccca aaggaagccc
     1741 gtgccctggg gtcccatcag ggccttgcgg ccgcagaagg cggcgaagtc gcagccgatc
     1801 tcatccaccc gcaccggtat ccgacccgca gactgggctg cgtccacgaa ggccagggcc
     1861 ccatgcgcgt gagcaatccg ggtgatctct gtcaccggct ggatggtgcc gaagacgttg
     1921 gagacgtggg tgcacgccac gagccgggtt gcgggcgtca gggcccggcc aacgtcgttg
     1981 gggtccacca agccgtcagg atcggcgggg acaaacctca ccctcccacc cttctcctgc
     2041 gcggcccgca tccaggggat ggtgtttgac tgatgctcca gctccgtcag aacgatctcg
     2101 tccccaggtt tccagtcgag gccctgggcc accaggttga tcgcctcggt ggtattcttg
     2161 gtgaaggcga tctcggcgtc cgcggcggct ccaacggcgt gcgcggcaat gtggcgggcc
     2221 gcgttcaccc gctccgcggc ttccagcgag cgcctccaca cgcccacgcc gtagttgaag
     2281 gggtggatgc agagatactc ctgcatggcc tcgatcactg ggcggggagc gggcaccacc
     2341 gaggcgctgt ccaggtacac caggtccttc agacagggca tgtctctgcg gatttcctcc
     2401 accgatttgg c
//
LOCUS       DBNGZC010000396         1968 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2380806, whole genome shotgun sequence.
ACCESSION   DBNGZC010000396 DBNGZC010000000
VERSION     DBNGZC010000396.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2380806"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..943)
                     /locus_tag="AC16G0_07025"
     CDS             complement(104..943)
                     /locus_tag="AC16G0_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HIR5099410.1"
                     /translation="MRKPYKTDLTDTQWKIIKPLIPPALPGGRPREVDMREVLNTLLY
                     QARTGCPWELLPHDLLPKSTVSDYFAQWRDDGTWQRITDALRKKLRVAKGREPTPRVA
                     YIDSQTVKGTESGGERGYDAGKKIDGRKRHIAFDSLGLLLAVVVTAASADDGATAPEV
                     LRQLNRPRFPRLATVWGDGKYRNHALASWLERTGKPFTVKVVERPKGSIGYVKLPKRW
                     VAERSFAWLGRDRRHSKDYERRTESSEAWIKISAIGGMTRRLAPDETRKPIPFKYARN
                     KAA"
     gene            complement(1026..1874)
                     /locus_tag="AC16G0_07030"
     CDS             complement(1026..1874)
                     /locus_tag="AC16G0_07030"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HIR5099411.1"
                     /translation="MAGLTGRKRGRPFRLGLGWVALLVTWVTTKTPGDFGFLRTRWSC
                     ALLALLLREREGVTASRETVRRWLHRGQLVYRRPRPVLKPDEEERRVKLAELRTLLEA
                     LPADETAVWQDEVEIHTNPKIGRMWMLKGHQAEVVTPGVNRKRHLSGSIHWRTGQVFV
                     TEAAPKQGRNGALFIAHLDELRRRLRRYKKIHVICDNASCHTSLEVIQYLWKHEGRIE
                     VHLLPSYSPDYNPIERIWWHLHEDITRNHRCRDLGELLEKVFAWLEQENPFEVEGSVY
                     PKAKAA"
BASE COUNT          314 a          664 c          621 g          369 t
ORIGIN      
        1 gtcaggatcg gttcatcagg ccggggcttg cggcggtacc tcacggtcga gcgggaccgc
       61 cccatcacgc cacagatccg gcgctgagag tctatccgga aagctacgct gccttatttc
      121 ttgcatactt gaatgggatc ggcttccgtg tctcgtcggg cgccagtcgc cgtgtcatcc
      181 cgccgatggc actgatcttg atccaggcct cactcgactc cgtgcgccgc tcatagtcct
      241 tgctatgacg ccgatctcgg cccagccagg cgaagctccg ttccgccacc caacgcttcg
      301 gcaatttcac gtagccgatc gagcccttgg ggcgctccac gaccttgacc gtgaacggct
      361 tccctgttcg ctccagccac gatgcgaggg cgtgattcct gtacttccca tcgccccaga
      421 cggtggccag ccgggggaac cgcggccggt tcaattgcct gagcacctcc ggcgccgtcg
      481 ctccgtcgtc ggccgaggcc gccgtgacca ccaccgccag cagcaacccc agcgaatcga
      541 aggcgatatg tcgcttccgc ccgtcgatct tcttgcccgc atcatagcct cgctccccac
      601 cactctcggt gcccttgacc gtctggctgt cgatatacgc gacccgcggc gtcggctcgc
      661 gtcccttggc cacgcgcagc ttcttccgca aggcgtcggt gatccgctgc caggtgccat
      721 cgtcgcgcca ttgggcgaag tagtcggaga cggtgctctt gggcaacagg tcgtgcggca
      781 gcaactccca cgggcagccg gtgcgggcct ggtacaacag ggtattgagg acctcccgca
      841 tgtcgacctc gcggggccga ccgccgggca aggcgggggg gatcaggggc ttgatgatct
      901 tccactgggt gtcggtcagg tcggtcttgt agggcttacg catcgaggga taggtcgctg
      961 tgcgaagggg tggactcctg ttcctacggg aattctaaat gaaaactttc aaaggggaaa
     1021 gtaaatcagg cggcctttgc cttcgggtag acggaaccct cgacctcgaa cgggttttcc
     1081 tgctccagcc aggcgaacac cttctccagt aattccccca ggtcgcgaca ccgatgattc
     1141 cgcgtaatgt cctcgtgcag gtgccaccag atccgctcga tggggttgta atccggcgag
     1201 taggagggca gcagatggac ctcgatccgg ccctcatgct tccagagata ttggatcacc
     1261 tccaggctgg tgtgacagct tgcgttgtcg cagatcacgt ggatcttctt ataccgtcgc
     1321 agtcgccgtc gcagctcgtc caggtgcgcg atgaacaggg ccccgttacg cccctgcttg
     1381 ggggccgcct cggtcacgaa cacctggccg gtccgccagt ggatcgaccc ggacaggtgc
     1441 cgcttccggt tcacgcccgg cgtcacgacc tccgcctggt ggcccttgag catccacatc
     1501 cggccgatct tgggattggt gtggatctcg acctcgtcct gccagaccgc cgtctcgtcg
     1561 gccggcaggg cttccaggag cgtgcgcagc tcggccagct tgacccgcct ctcctcctca
     1621 tcgggcttga ggaccggccg gggtctccgg tagacgagtt ggccgcggtg gagccagcgg
     1681 cggaccgtct cccggctggc ggtgacgcct tcccgctcgc gcaacagcag ggccaacagc
     1741 gcgcaggacc agcgggtgcg gaggaagccg aagtcgccgg gggtcttggt ggtcacccag
     1801 gtgacgagga gggcgaccca gccgagcccc agccgaaagg ggcgaccacg tttcctcccg
     1861 gtgagccccg cgatcccctc ggcctggaac cgcttgagcc agcggtcgac cgtgcggagc
     1921 tacagaagag catggcctcg atggtatccc aggcgtggcc ggcgccca
//
LOCUS       DBNGZC010000397         2055 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_716676, whole genome shotgun sequence.
ACCESSION   DBNGZC010000397 DBNGZC010000000
VERSION     DBNGZC010000397.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_716676"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="AC16G0_07035"
     CDS             <1..370
                     /locus_tag="AC16G0_07035"
                     /inference="COORDINATES: protein motif:HMM:NF015962.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HIR5099412.1"
                     /translation="FLNRSAVGEWLIAGRADALIVCSGYEGVFSLEDAVCAGAIVDRA
                     AALGRGLVLGDGARACQALWARFGSNLPQLLRETEWGRHIVGLGLEVDLDICASLDAT
                     NVLPRMERGRIRLQTPRPSN"
     gene            622..1308
                     /gene="tmk"
                     /locus_tag="AC16G0_07040"
     CDS             622..1308
                     /gene="tmk"
                     /locus_tag="AC16G0_07040"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR5099413.1"
                     /translation="MTEEPKENLPGTLIAVEGLDGSGKSTQVHLVHRWLQSKGYRVFF
                     SEWNSSVLVREATRKGKKRQLLTPTTFSLIHATDFADRYERQILPLLRAGYLVLCDRY
                     IFTAFARDAVRGCDPEWLRNLYGFARIPDITFFFRLPLDVALGRILEGRPQLKYFEAG
                     MDLNLSPDLTESFRIFQGRVLAEYLGMVDEFGFTVVDATQSVHRQQLSVRRLIEGRVD
                     LGFFHKRYVP"
     gene            1305..2039
                     /locus_tag="AC16G0_07045"
     CDS             1305..2039
                     /locus_tag="AC16G0_07045"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR5099414.1"
                     /translation="MSPKRTYGMAPAGVDPDTLSGTLIVIEGPDSSGRSTQVALLAQW
                     LEEQGYAVAQVGLKRSTLVGPELEQAKQGNILSPRTLSLFYATDFYDQLENRIVPSLR
                     AGFVVLADRYIYTLIARAIVRGADPEWVASVYSMAIVPDAVFYMTASFTTLVQRTFQA
                     HTELDYWESGMDCGLSRDWFQSFVRYQRRIRSEFFKMQKKYAFELVNANRSIAAVDRD
                     LKGRIQLVLDRAEKPEERRVRRARVR"
BASE COUNT          359 a          644 c          680 g          372 t
ORIGIN      
        1 gttcctcaac cggtctgccg tgggcgaatg gctaatcgcc gggcgggcgg acgccctgat
       61 cgtctgctct gggtatgaag gagtgttctc gctcgaggat gcggtatgtg cgggagccat
      121 cgtggatcgg gcggcagccc tggggcgggg cctcgtcctc ggcgacggcg cgcgggcgtg
      181 tcaggcgctc tgggcgcgct tcgggtccaa tctgccgcaa ctgctgcgcg agaccgagtg
      241 gggacggcac atcgtcgggt taggcctcga ggtggacctg gacatctgcg ccagcctgga
      301 tgcaacgaac gtgctccccc ggatggagcg ggggcgaata cgcctgcaaa caccacggcc
      361 ctcgaattga ccggaccgtc cagaggtcca ctcgagatgc ccgaggcgtt ggatcgcacc
      421 catgggtgac cgtggggtga tcgtggcggg gagggaggct ggaatcctgg ggcactcccc
      481 gcctcagagc cgggactgcc ttgcaaagat ggctgaacca ttgtaaagat gatggccatg
      541 gcggtacgcc tagcctgggg acctccttca gcgggccttc gtccagccgc tgaatttctg
      601 taggcgagag aggagaaatg catgacggag gagcccaagg agaatcttcc cggcacgctc
      661 atcgccgtgg agggcctcga cggatcgggc aagtccaccc aggttcacct ggtccaccgc
      721 tggcttcaga gcaaaggcta ccgggtgttc ttctcggagt ggaactcctc cgtcttggtc
      781 cgggaggcca cgcgtaaggg caagaagcgg caactcctca cccccaccac cttcagcctc
      841 atccacgcca cggattttgc cgaccggtac gagcggcaga tcctgcccct cctgcgggcg
      901 ggctatctgg tcctgtgcga ccggtatatt ttcacggcgt ttgcgcggga tgcggtgcga
      961 ggctgtgacc ccgaatggct gaggaacctg tacggcttcg cgcggatccc ggacatcacc
     1021 ttcttcttca gactccccct cgacgtggcc ttggggcgga tcctggaagg ccgaccgcaa
     1081 ctcaagtact tcgaggccgg catggatctg aatctgagcc ccgatctcac cgagagcttc
     1141 cggatcttcc agggacgggt cctcgcggaa tacctgggca tggtggacga gttcgggttc
     1201 accgtcgtgg acgccaccca gtcggtccat cgccagcaac tcagcgtgcg acgtttgatc
     1261 gagggccggg tggacctggg cttcttccac aagaggtacg tgccatgagc cccaaacgca
     1321 cctacggcat ggccccggca ggtgtggacc cggacaccct ctcgggtacc ctgatcgtca
     1381 tcgagggacc cgattcctcg gggcgctcca cccaggtcgc cctcctggcc cagtggctgg
     1441 aggaacaagg ctacgcggtg gcccaggtcg ggctcaagcg atccaccctg gtgggtcccg
     1501 agttggagca ggccaagcag gggaacatcc tcagcccccg cacgctcagc ctcttctacg
     1561 cgacggactt ctacgaccag ttggagaatc gcatcgtccc gtccctgcgg gcgggcttcg
     1621 tcgtcctcgc ggatcgctac atctacacgc tcatcgctcg ggccatcgtg cggggtgcgg
     1681 accccgagtg ggtggcctcg gtctactcca tggccatcgt ccccgacgcg gtcttctaca
     1741 tgacggcctc cttcaccacc ctggtgcagc gcacgttcca ggcccatacc gagctggatt
     1801 actgggagtc gggcatggac tgcggtctga gccgggactg gttccagagc ttcgtgcgct
     1861 accagcggcg aatccgcagt gagttcttca agatgcagaa gaagtatgcc ttcgagctgg
     1921 tgaatgccaa tcggagtatc gccgccgtgg accgggacct gaaagggagg attcaactgg
     1981 ttctcgaccg tgcagaaaag cccgaggaga ggagggtgcg ccgtgcacgc gtccgctagg
     2041 aagaccaagg aggag
//
LOCUS       DBNGZC010000398         2232 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2576950, whole genome shotgun sequence.
ACCESSION   DBNGZC010000398 DBNGZC010000000
VERSION     DBNGZC010000398.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2576950"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1402
                     /locus_tag="AC16G0_07050"
     CDS             <1..1402
                     /locus_tag="AC16G0_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099415.1"
                     /translation="KRVILGLMLASFVTPPFLGAFAWVMLAGPNAGILNKLYRSLTGS
                     EEPLVNIFSMPGLIFVVAIYTFPYVFIMVANTLDLIASDLEEAAAMLGAGRIQVALTI
                     TIPLVAPAILSGFILAVLQALALFGSPAILALPAGFHTITTQIWALFQYPPKTEMAAA
                     FSIPLLLATALLLLLQKRLLGRRGYASIGGKGGQRRRIPLGAWRYPALLGCLAVMAGS
                     IFLPYGILTKAALSRAWAQPMTWENLTLANFRFTFFEYSSTRLAIVNTLELAILTACV
                     GAGLVAIPAYITNRRIILGHQVLGFLVLAPIVIPGVVLAVGLFVSYTRPPVLLYGTLW
                     ILFVAYLTKEMPVGYSQSDATFRGIHPELEEAGRILGAGRLRVLREITVPLALSGIIA
                     TWCFIFIGVIRELSASILLFTPNTKVLSVVIFDLKEEGQFGAISVLGLFMLAVTFAIV
                     ILMQNLLARDFLATKE"
     gene            1514..>2232
                     /locus_tag="AC16G0_07055"
     CDS             1514..>2232
                     /locus_tag="AC16G0_07055"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099416.1"
                     /translation="MPGVTIKGLAKRYGDVAALERLDLEVKPGELVSLLGPSGCGKTT
                     TLRLVAGFLTPEDGEIWIGDRCLSSRTMVVPPERRRMAMIFQSYALWPHMSVAQNVAY
                     GLRFKKGIAKAERDRRVKEMLKVVQLARFESRYPGELSGGQQQRVALARSLVVEPEIL
                     LLDEPLSNLDANLREEMRFEIRRLHEAFGITTLYVTHDQAEAMVISDRIAVLRGGRAV
                     QVGTGEDLFQRPRSRFVAEFIG"
BASE COUNT          354 a          742 c          694 g          442 t
ORIGIN      
        1 aaaacgtgtg atcctgggcc tcatgctggc ctcgtttgtt acgccgccat tcctgggggc
       61 gtttgcctgg gtcatgctcg ccgggcccaa cgccggaatc ctcaacaagc tctacaggag
      121 cctgaccgga tccgaggagc ccctggtcaa catcttcagc atgccgggac tgatcttcgt
      181 tgtggccatc tacacattcc cctatgtctt catcatggtc gccaacacgc tggacctgat
      241 cgcctctgac ctggaggagg ctgccgccat gctgggggcc ggccgcatcc aggtcgccct
      301 cacgattacc attcctctgg tggccccggc catcttgagc ggcttcatcc tggcggtcct
      361 ccaggccctg gcgctcttcg gatcgccggc catcctggcc ctgcccgcgg ggttccacac
      421 catcaccacc cagatctggg ccctcttcca gtacccgccc aagacagaga tggctgcggc
      481 cttttccatc ccgctcctgc tggccacggc gctgctcctg ctgctgcaga agagactcct
      541 cgggcgccgc gggtacgcct cgattggcgg caagggcgga cagcggcgga ggatccccct
      601 gggagcctgg cgctatcccg cgctcctcgg ctgcctggcc gtcatggcag gctccatctt
      661 ccttccgtac ggcatcctga ccaaggccgc cctctcccgg gcctgggccc agcccatgac
      721 gtgggaaaac ctcaccctgg caaatttccg gttcaccttc ttcgagtaca gctccacccg
      781 gctggccatc gtcaatacgc tggagttggc gatcttgacc gcctgcgtgg gggccgggct
      841 cgtggcgatc ccggcctaca tcacgaaccg caggattatc ctcggccacc aggtcctggg
      901 gtttctcgtg ctcgctccca tcgtgatccc cggtgtggtc ctcgccgtgg gtctattcgt
      961 ctcctacacc cggccgccgg tcttgctcta cggcacgctc tggattctct tcgtcgcgta
     1021 cctcaccaag gagatgcccg tgggctattc ccagtccgac gccacgttcc ggggaatcca
     1081 cccggagctg gaggaagccg ggcgcatcct gggggcgggg cggctgcgag ttctccgcga
     1141 gatcacggtc ccactggccc tgagcgggat catcgccacc tggtgcttca tcttcatcgg
     1201 ggtcattcga gagctgtcgg cctcgatcct gctcttcacg cccaacacca aggtcctgtc
     1261 cgtggtgatc ttcgacctca aggaggaggg ccagttcggc gccatctcgg tcctgggtct
     1321 cttcatgctg gccgtcacct ttgccatcgt cattctcatg cagaacctgc tggcgcgcga
     1381 cttcctggcg acgaaggagt gagcacgagc aaacttcaat gaccaatgac cacatcccaa
     1441 tgaatccagg ttgctccgaa ctcattggcc ttcggtgctt gaccatctgt catccggctt
     1501 gggggcgtga acgatgcccg gtgtgaccat caagggcctg gccaagagat acggggacgt
     1561 ggcggcgctg gagaggctgg acctcgaagt gaaaccgggg gaactggtgt cgcttctggg
     1621 gccgtcgggg tgcgggaaga cgacaaccct gcgcctcgtg gcgggctttc tcacgcctga
     1681 agacggagag atctggatag gcgaccgctg cctctcctcc aggaccatgg tggtcccgcc
     1741 ggagcgccgc cgcatggcga tgatctttca aagctacgcc ctctggcccc acatgagcgt
     1801 ggcccagaac gtggcctatg gcctgcgctt caagaaagga atcgccaagg cggagcggga
     1861 ccgccgcgtg aaggagatgc tgaaggttgt ccagcttgca cggttcgagt cccgctaccc
     1921 gggggagctg tccggaggac agcagcagcg ggtggcgctg gcccgatctc tggtcgtgga
     1981 gccggagatt ctgctgctgg atgagcccct cagtaacctg gatgccaacc tgcgagaaga
     2041 gatgcgattc gagatccgcc ggctccacga ggcgtttggg atcaccacgc tgtatgtgac
     2101 ccacgatcag gcggaggcga tggtcatctc ggaccggatc gcggtcctgc ggggcggccg
     2161 ggcggttcag gtagggacgg gggaggacct cttccagcgt ccccggagcc gcttcgtcgc
     2221 ggagttcatc gg
//
LOCUS       DBNGZC010000399         1498 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2380988, whole genome shotgun sequence.
ACCESSION   DBNGZC010000399 DBNGZC010000000
VERSION     DBNGZC010000399.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2380988"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1297)
                     /locus_tag="AC16G0_07060"
     CDS             complement(44..1297)
                     /locus_tag="AC16G0_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5099417.1"
                     /translation="MLALSLSELNRDGRRLFIARMARMFGYGFLAVVLVLYLAALGFD
                     ETRIGLLLSLTLVGDTVISLWLSTHADRYGRRRMLVLGALLIVGAGLVFLATGQFLLL
                     LAAATIGVISPSGYEVGPFLAIEQAALSQTVAPERRTGVFAWYNMAGSFATAAGSLLG
                     GGIARLGQAGGLTPLASYRIVLAGYALFGVLLAGLFWRLGPSVEVSPASAPIPASSVS
                     RLGLHRSAGVVLRLSGLFAMDAFAGGFVIQSILAYWFYLRFGTNEATLGMIFFGANLL
                     AGVSALAASVIAARFGLVNTMVFTHLPSNILLILVPLMPTLPLAILVLLLRFSISQMD
                     VPTRQSYTMAIVRPEERSAAAGITGTARTVGAAVAPVCAGFLLGDPHLMSGLFFLAGG
                     LKIVYDFLLLRGFRRVRLPEEGAPS"
     gene            complement(1304..>1498)
                     /locus_tag="AC16G0_07065"
     CDS             complement(1304..>1498)
                     /locus_tag="AC16G0_07065"
                     /inference="COORDINATES: protein motif:HMM:NF014653.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HIR5099418.1"
                     /translation="EMDRVIREIRDSEKMDGVNRIWLPGEMEFCRIRERREKGIPIAA
                     AVHDELHKLATELQVPDRLE"
BASE COUNT          319 a          511 c          471 g          197 t
ORIGIN      
        1 aggcacctcc cgattcgacc cacctcgccc ggcgacgcct ccgtcacgat ggcgcaccct
       61 cctcgggaag cctcaccctg cggaagcccc ggaggagcag gaagtcatag acaatcttga
      121 gccctccagc caggaagaag aggccgctca tcagatgtgg gtctcccagc aagaagccgg
      181 cgcagaccgg ggccaccgcg gcgcccacgg tgcgcgcggt cccggtaatc ccggcggcgg
      241 ccgagcgttc ctccggccgg acaatggcca tggtgtacga ctgccgcgtg ggaacgtcca
      301 tctgggagat gctgaaccgc agcagcagga ccaagattgc caaaggcaat gtcggcatga
      361 gcggcacgag gatgaggaga atgttggagg ggagatgggt gaagaccatg gtattcacga
      421 ggccgaagcg ggccgcaatg acggatgccg ccaaggccga cacgccggcc aggaggttcg
      481 ccccgaagaa gatcatcccc aatgtcgctt cgttggtgcc gaaccgaaga tagaaccagt
      541 acgccaggat gctctggatc acgaaccccc cggcgaaggc atccatggcg aagaggccgg
      601 acagccggag caccacccct gccgaacggt gaaggcccaa tcgggaaacg gatgatgctg
      661 ggatcggcgc tgaggccggg gagacttcca ccgatggacc gaggcgccag aacagtcctg
      721 ccagcagcac gccgaagagc gcgtagccgg ccaagacgat gcgatagctt gccaggggcg
      781 tgagtccccc cgcttgcccc agtcgggcga tgccgccccc cagcagcgat ccggccgcgg
      841 tcgcgaagga gcccgccatg ttgtaccagg cgaaaacccc cgtccgccgc tcgggggcca
      901 cggtctgaga cagggccgcc tgctcgatgg ccaggaaggg tcccacctcg tacccgcttg
      961 gactgatgac gccaatcgtc gccgccgcca gcagcagcag gaactgcccc gtggccagaa
     1021 agacgaggcc ggcccccacg atgaggaggg cccccaggac gagcatgcga cgccgcccat
     1081 accggtcggc gtgcgtgctc aaccacaggg agatgaccgt gtcaccgacc agggtgagcg
     1141 acaagagcag gccgatgcgc gtctcatcga accccagggc ggccaggtac aagacgagga
     1201 cgacggccag gaagccgtag ccgaacatcc gcgccatccg cgcgatgaac aaccggcgtc
     1261 cgtcccgatt cagctcactg agagacaagg ccagcattcg tccctactcc aaccgatcgg
     1321 gcacctgcaa ctccgtggca agcttgtgta gctcgtcatg gacggccgcg gcgatcggaa
     1381 tacccttctc ccgtcgttcc ctgatccgac aaaactccat ctcccccgga agccagatcc
     1441 gattcactcc gtccatcttc tccgagtcac ggatctcccg gatgactcga tccatctc
//
LOCUS       DBNGZC010000400         7978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_914164, whole genome shotgun sequence.
ACCESSION   DBNGZC010000400 DBNGZC010000000
VERSION     DBNGZC010000400.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7978
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_914164"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="AC16G0_07070"
     CDS             complement(<1..204)
                     /locus_tag="AC16G0_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099419.1"
                     /translation="MDSLAGIAVTSFLVGLSGAIMPGPVLTITIGETAARLRTGGLGG
                     DPVPADLTGPASQREGSNKNGRAL"
     gene            complement(484..834)
                     /locus_tag="AC16G0_07075"
     CDS             complement(484..834)
                     /locus_tag="AC16G0_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099420.1"
                     /translation="MIYAANEPGGVDARLGNLAEELQRTFRYSMYRLLEAPQGSAALN
                     QSWRVGLPGDRTLEVIPTAIQGGQYNLTVHVLGPGGQALVNTQVRLRGGGPPVSVGGL
                     PHEKGVLIIAISAG"
     gene            complement(975..1490)
                     /locus_tag="AC16G0_07080"
     CDS             complement(975..1490)
                     /locus_tag="AC16G0_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HIR5099421.1"
                     /translation="MKHWLHKVMGRTRRLAMYATGEMAETQQGRMRVLLAGCPACRRE
                     VEAYRLLSDTLRALPQVRLSPGEAAVFWPSVEGRIRQGETPAARPARPSFRELYWDHP
                     RLSLISATAAIVLVLGLTLGPRVGWGPGTHNSNGTEVISVEAGENTSVMLFQAPDSSL
                     KFIWVFETPSS"
     gene            complement(1523..2113)
                     /locus_tag="AC16G0_07085"
     CDS             complement(1523..2113)
                     /locus_tag="AC16G0_07085"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR5099422.1"
                     /translation="MAGADEPEDDWNLVDSAKRGDRNSFGTLVHRHQDQMFNLAYQIV
                     RNREDALDIAQEAFSKAFVSLPHFKGDASFTTWMHRIVVNLAIDSLRRKQREGQTSYD
                     DTRGTSEEHQGAIVAPDDPSEILEAKQVRVLLARGIAKLPPAQRAVLILREIEGMTYD
                     EIARTVGCTLGTVMSRLFYARQRLRRLLAGHRTDLR"
     gene            2372..3547
                     /locus_tag="AC16G0_07090"
     CDS             2372..3547
                     /locus_tag="AC16G0_07090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5099423.1"
                     /translation="MAFTLTETVASLVLAFWLWAAWTCWRGQKRLPYLAPAEGSPGPH
                     AALSVIIPCRNEADSLPRSVPSLINQTYPNLEVILLDDRSTDGTGQVLQTLAQTCSRL
                     RTIQIRTLPEGWLGKTHALWFGSQQARGSWLLFTDADVIFHPQCLEAAISHAETHHLD
                     HLVVIPRVETVGFWETILVSCFGLLFGLALRPWQVSNPRSSAHIGIGAFNLIRHSAYL
                     AIGTHRALANAVVDDLELGRRVKQAGLRQAAARAGDLLRVRWQVGLAGVVSGLEKNAF
                     AGVGFSLLRATATCILLAGLGILPFVGAMLDPARGLWAAAALTVSALQAAHARNAGLP
                     VWSGPFHPLATAVLIYAVARSTFMALHRGSVVWRGTSYPLTSLRRPPPVLEAARTKH"
     gene            3759..5123
                     /locus_tag="AC16G0_07095"
     CDS             3759..5123
                     /locus_tag="AC16G0_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099424.1"
                     /translation="MSRFWRYRQSGAWMLLAAVLVLMPCPVRAASAPPFAWRQFAGTT
                     LHVLLSKSPWQQVMSRHFPEFEELTGIHLALEVFPQAELWNVLEKGLHEPGRVDVFMT
                     VPGLDGLRFLRAGGIQPVNDYLKDPRLTAQDYDWNDFLPKTRAAMELDGAILGPPIMV
                     ENLALLYRKDLFKKYQVGVPRTLEELEAAARFLHKKPMGPKGEAGVAIVSRGQGVMAT
                     SLYAGILQALGGSWLDGDHRPTIDKQRGLAALEYLGRLLGSYAPPNISEFGWQEATKV
                     FQESKAAMYIEGSSIYPLIEQPETSEVAGKVGYALFPSGAGGPGTLIAARGLALGKQS
                     RQPQAAWLFMQWASGKQMVKEVLQRGVLVGRESAWRDKDANWKVPADLAQTFQEAGRI
                     GGVEWAPPMIAVTSARETVGDAITAAIRGNDVHAAATTAAKRLTEILNTTEGAQRRPT
                     RVTP"
     gene            5348..6670
                     /locus_tag="AC16G0_07100"
     CDS             5348..6670
                     /locus_tag="AC16G0_07100"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099425.1"
                     /translation="MSNQRWYVALGLIVGLLVASSAQAAQFNWQQFKGTQLRVLLNKH
                     PWQAGIEPHLKEFEALTGIKVILEVYHEDQFRAKTLVELTSGASSMDVFMTMPGQEGL
                     KYMRAGWYQPVDEFLKDPSLTSSDYSWNDFLDKARDAMVIDGKTIGPPIQIENSALMY
                     LKDVFQKYNVKVPTTLDELEAAAKALNGKAMTDDGQPGFGFVARGKRAAATSQFAGML
                     FAMGGTWLTQNREPAINSPEAVKALDMWGRLLRLYGPPGSENNHWYEASSIFSQGKAA
                     IWLDANSLFPVIEDPQKSKIIGKVGYAVFPKGPNNHPGGTVAPWGLGIPKTSKNQKAA
                     WLFLQWATNKEQVLKLQSGAGVLGARDSVWKDQKGRGKVPADLAESLAQGSKVGTPLW
                     NPPVVAVAEVRDAIGNAIVTSIQGGDVKAAADKAAADMKRIMAETEKK"
     gene            6927..>7978
                     /locus_tag="AC16G0_07105"
     CDS             6927..>7978
                     /locus_tag="AC16G0_07105"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099426.1"
                     /translation="MATTRRVWSLGLIVGLLGLFLASVGETAQFNWQQFKGTQLRVML
                     NKHPWQIAIEPHLKDFEALTGMKLVVEVYPEDQFRAKTSVELQSGSGSIDVFMTMPAQ
                     EGLKYLRAGWYQPIDEYLKDASITSPDYNWNDFLEVGRSAMTVDGRIIGPPIQLETNQ
                     LMYRKDVFQKYNVKVPTTLDELEAAAKALNGKAMTDDGQAGFWFVARGKRAAATSVFS
                     GYLHAMGAAWLGPTREPIFNSDEGIKAFELYGRLLRLYGPPGSENNHWYEASSIMGQS
                     KAAMYTEFDSISAFVEDPEKSRVKGKMGYVMFPKGPNGKPGTMATVWGLGIPKNSGNP
                     KAGWLFLQWATSKETVL"
BASE COUNT         1492 a         2563 c         2503 g         1420 t
ORIGIN      
        1 cagggcgcgc ccgttcttgt tcgatccctc tcgttgactg gctggtcccg tcaggtcggc
       61 gggtactgga tcaccgccca ggcccccggt tcgcaggcgt gcggccgtct cgccgatcgt
      121 gatcgtgagg accggcccgg gcatgatcgc cccggacagg ccgacgagga acgaggtgac
      181 tgcgatgcca gccaggctgt ccacccacac ccccactagc gtggtcttcg caaaaagtga
      241 ccgaaatatg tatagcttca atcttgagac cgcgcaaggg aatttcccga cttggatgcg
      301 taggaacacc attgctggat gggctgcgga aagaaatgac gggcgctcgc gtgagcgccc
      361 gtgagggaag gcataccatc gcttcggtgc ttaacccgat cagacactga agcggctgat
      421 atggcaggct gttgaataac ctttcttccg cccaagctgt ccgggacaac tcacgggcgg
      481 ttcttagccg gccgagatgg caatgataag aacgcctttt tcgtggggta gccctccaac
      541 cgaaaccggc ggaccgccgc cgcgcaggcg aacctgagtg tttaccaggg cttgcccccc
      601 gggccccaag acgtgaaccg tcaggttgta ctgtcccccc tggatcgccg tcgggataac
      661 ctcgagggtc cgatcccccg gcaaaccaac ccgccaagat tgattcagcg ccgccgatcc
      721 ctgcggcgcc tccaggagtc ggtacatgga gtaacggaat gtccgctgaa gctcttccgc
      781 cagattcccc agcctcgcat ccacaccgcc aggctcattc gcggcataga tcacctgagc
      841 ccggagggca accacttgct gggcccaggc cccgattggt gagccgccca aatgaaccac
      901 cgccagaccg acgcaaaaaa acgcaagaca cctgcaccgc gggagttgct ccacccggtt
      961 gcctccccgt tgtcttacga agaaggagtc tcgaacaccc agataaactt gagcgagctg
     1021 tcgggcgcct gaaacagcat cacagatgtg ttctcacccg cctcgaccga aatcacctcc
     1081 gtgccgtttg aattgtgcgt gccaggaccc cagccaaccc tcgggcccag cgtaagaccc
     1141 agaaccagga cgatcgcagc cgttgccgaa atcaggctca atcggggatg atcccagtaa
     1201 agctcgcgga acgacggccg cgccggtcga gccgccgggg tctcgccctg ccggatcctg
     1261 ccttccacgc tgggccagaa aaccgcagcc tctccgggag acagcctgac ctgcggcaat
     1321 gcccggagag tgtcagacag cagacggtag gcctcgacct cgcgccggca ggccggacaa
     1381 cccgcgagaa ggactcgcat ccggccttgc tgtgtctcgg ccatctcgcc cgtcgcgtac
     1441 atggccagtc gccgggtccg acccatcacc ttgtggagcc agtgcttcat ctcaatcgac
     1501 caatctcgac ccaacgggcg gctcagcgaa gatccgtacg gtgcccggcg agcagcctgc
     1561 gaagcctttg ccgggcatag aataagcggg acatcacggt gcccaaagtg catcccacgg
     1621 tccgggcgat ctcatcgtag gtcatcccct cgatctctcg caggatcaag accgcccgct
     1681 gcgccggcgg aagcttggct atcccccgag ccagcaggac ccggacttgc tttgcctcca
     1741 ggatctcgga gggatcgtcg ggagcaacga tcgcgccctg atgttcctcc gaagtaccac
     1801 gcgtgtcgtc ataactcgtc tggccctctc gctgcttccg tcgcagacta tcaatcgcga
     1861 gattcaccac aattcggtgc atccaggtcg tgaacgaggc gtcacccttg aagtgtggga
     1921 gggacacaaa agccttggaa aaggcctcct gcgcgatgtc cagggcatcc tcccggttgc
     1981 ggactatctg gtacgcgaga ttgaacatct ggtcctggtg gcgatgaacc agggtgccga
     2041 aggagttacg gtctccccgc ttggccgagt ccaccaggtt ccagtcatcc tcgggctcgt
     2101 cagctcccgc catccccctc cttaccttgg acgcgtcgat atgcccttta ttcaatacct
     2161 tagctgcgga tgctaccacc cgtgacggcg ggtaggcaag gggaattccg gtcgagctga
     2221 tccatgtcgg agaacgaaag cttcccggcc aagttgaggg cggatatgag tggacaggtc
     2281 ctcatggcgc cactatactc ccgatctagc cgctgagcgt ctgcgggccg atgatcacgg
     2341 atggcctcaa gcagactgaa gaaatccctc catggccttt accttgactg aaaccgtggc
     2401 aagcctcgtc ctggccttct ggctctgggc agcttggacg tgctggcgag gccagaagcg
     2461 cctcccgtat ctcgcgcctg ctgaaggatc gcctggccct cacgcggctc tctcggtgat
     2521 catcccttgt cggaacgagg cggattccct gccacggtcc gtgccctctc tgattaatca
     2581 gacctatcct aatctggagg tgatcctcct agacgaccgg tccacggatg gcacgggcca
     2641 ggtcctccaa acgctcgcgc aaacctgctc ccggctgaga acgatccaga ttcgcaccct
     2701 gcccgaggga tggctcggga agactcacgc cctctggttc ggcagccagc aggcccgggg
     2761 aagctggctc ctgttcaccg acgccgacgt gatcttccac ccgcagtgcc tggaggctgc
     2821 catctcccat gcggagaccc atcacctgga ccacttggtg gtcattccca gggtcgagac
     2881 ggtgggtttc tgggagacga tcctcgtgag ctgctttggc ctgctgtttg gcctggcgct
     2941 gcgaccctgg caagtcagca atccacggtc gagcgcgcat atcgggattg gcgccttcaa
     3001 cttgatccgt cactcggcct atctggccat cgggacgcat cgcgccttgg cgaacgccgt
     3061 cgtggatgac ctcgaactcg gcaggcgggt gaagcaagcc ggtctgcgtc aggccgccgc
     3121 ccgcgcgggg gacctcctcc gggtgcgctg gcaggtcggc ctcgcgggcg tggtatccgg
     3181 cttggagaag aacgccttcg ccggcgtagg cttttctctg cttcgcgcaa cggcgacctg
     3241 catcctgctg gcgggcctgg gaatcctccc gttcgtgggg gcaatgcttg atcccgcccg
     3301 gggcttatgg gcggcggcgg cgttgaccgt gtccgctctc caggccgctc acgcccgcaa
     3361 cgcggggctt cccgtctggt ctggcccgtt ccatccgctg gccaccgccg tcctcatcta
     3421 cgccgtcgct cgctccacct tcatggccct ccatcgcggg agcgtggtgt ggcgcgggac
     3481 ctcgtacccg ctgaccagcc tgcgtcgtcc tccgccggtc ttggaggcgg ctcgcacgaa
     3541 gcattgacag ggaccagccg aagagatagg ttgcccttgt tcttcaggga caggccgcgg
     3601 acactccgta cgtgggggct ctccccggat gcctgcatcc agcaggtggc gaaaccggca
     3661 gcgtatcggt gcaccgcttc ggactctccc gcggcaccca agtttctgag atgatcgccg
     3721 gcgcaaccac ctgcgcgggt gtgacccgga gggagacgat gtcacgattc tggcgttatc
     3781 ggcagtccgg cgcgtggatg ctgctggctg ccgtccttgt cttgatgccg tgcccggtcc
     3841 gcgctgcctc cgcgccgccc ttcgcctggc ggcaattcgc aggaacgact ctccacgtcc
     3901 tcttgagcaa gagtccctgg cagcaggtca tgagccggca cttccccgag ttcgaggagc
     3961 tcaccgggat tcacctggcg ctggaggtgt tcccccaagc cgaactatgg aatgtgctgg
     4021 agaagggtct gcacgagccg gggcgggtgg acgtcttcat gacggtcccc ggcctggacg
     4081 gtcttcgctt ccttcgtgcc ggcggcatcc agccagtcaa cgactacctc aaggacccgc
     4141 gcctcaccgc gcaagattat gactggaacg actttctccc caagactcgt gcggccatgg
     4201 agctggatgg cgccatcctc ggaccgccga tcatggtgga gaacctggcg ttgctgtatc
     4261 ggaaggacct cttcaagaaa taccaggtcg gcgttcctcg gaccttggag gaattggagg
     4321 ctgcggcgcg cttcctccac aagaagccca tgggtccgaa aggggaggcc ggtgtggcca
     4381 tcgtcagccg gggccagggg gtcatggcca cctcgctgta cgccgggatc cttcaggccc
     4441 tgggaggctc ctggttggac ggggaccatc ggcccacgat tgacaagcaa cggggcctgg
     4501 ctgccctgga gtacctcgga cggcttctgg ggagctacgc accgccaaac atctcggagt
     4561 tcggctggca ggaagcgacg aaggtgttcc aggaaagcaa ggcggcgatg tacatcgagg
     4621 ggagctcgat ttatccgctc atcgaacagc ccgagacctc cgaggttgcc ggcaaggttg
     4681 gctatgccct cttcccgtcc ggagccggtg gacccggcac gctcatcgct gcccgggggc
     4741 tggctcttgg caagcagagc cggcagcccc aggcggcttg gttattcatg cagtgggcca
     4801 gcgggaagca gatggtgaag gaggtgcttc agcgcggcgt cctggtcggc cgcgagtcgg
     4861 cctggcggga caaggatgca aactggaagg tcccggcgga cctggctcaa accttccaag
     4921 aggccggacg gatcggcggg gtggaatggg ctccacccat gattgcagtg acctcggcgc
     4981 gcgaaaccgt gggggacgca atcaccgcag ccatccgagg caacgatgtt cacgccgctg
     5041 ccaccaccgc tgccaaacgg ctcacagaga tcttgaacac gaccgaaggt gcgcagaggc
     5101 gcccgacgcg cgtgaccccc tgagaggaca ccaccggaag gcgcgattgc ggaccgacgt
     5161 tcccgcagga ggaactcgtg tcgtgggtgg tcctgcaaat cggacatctc cgattcccgg
     5221 tgccaccgaa cttgaatcct cagcccatcc gtgttaggta tgctgtcacg cgccggggtg
     5281 agagagccgg cgtggatcgg ccgtgttcgg caggtggtga cccccggagg gaaagaggag
     5341 gaattgcatg agcaaccagc ggtggtatgt ggctctgggc ctgattgtgg ggcttctggt
     5401 ggcctcgtcg gcgcaggccg cccagttcaa ctggcagcag ttcaagggga cgcagcttcg
     5461 cgtccttctc aacaagcatc cctggcaagc cggcatcgaa ccgcacctga aggagttcga
     5521 ggccctcacc gggatcaagg tcatcctcga ggtctaccac gaggaccagt tccgcgccaa
     5581 gacgctcgtg gagctcactt ccggcgccag cagcatggat gtcttcatga ccatgccggg
     5641 gcaggagggg ctgaagtaca tgcgggccgg ctggtaccag ccggtggatg agtttctgaa
     5701 agatccatcc ctcacctcct ccgactatag ctggaacgat ttcctcgaca aagcacgcga
     5761 cgccatggtc atcgatggaa agacaatcgg cccgccgatc cagatagaga actcggccct
     5821 catgtacctg aaggacgtgt tccagaagta caacgtgaag gtcccgacca ccttggacga
     5881 gctggaggcc gctgccaagg cgttgaacgg caaggccatg acggatgacg ggcagcccgg
     5941 cttcggattc gtggctcgtg gcaagcgggc cgcggccacc tcccagtttg cgggcatgct
     6001 tttcgcgatg ggcggcacct ggctgacgca gaaccgcgag cccgccatca atagtccgga
     6061 ggccgtcaag gccctcgaca tgtggggccg cctgctgcgc ctgtatgggc ctccgggctc
     6121 cgagaacaac cactggtacg aggccagttc catcttctcc cagggcaaag cggcaatctg
     6181 gctggatgca aactccctgt tcccggtgat cgaggacccg cagaagtcca agatcatcgg
     6241 taaggtgggg tacgccgtgt tcccgaaggg ccccaacaac catcccggtg gaaccgtggc
     6301 cccctggggg ctgggcatcc caaagaccag caagaaccag aaggccgcct ggctcttcct
     6361 gcagtgggcc acgaacaagg agcaggtgct caagctccag agcggagcgg gagtgctcgg
     6421 cgcccgcgac tccgtgtgga aggaccagaa gggtcggggg aaagttccgg ccgatctggc
     6481 cgagtccctg gcccagggtt ccaaggtcgg cacgccgctc tggaacccgc ccgtggtggc
     6541 cgtggccgaa gtccgcgacg ccatcgggaa cgccatcgtg acctcgatcc agggtggcga
     6601 cgtgaaggcg gcagccgaca aggccgctgc cgacatgaag cgcatcatgg cggagaccga
     6661 gaagaagtag caccgacgct gttggcggaa ttcgcatgga ggaggcccaa tcgggcctcc
     6721 tcttttttcg tgccccagat cgtccatcgc cacagcggtg acgatccggc ccccgaatcg
     6781 tgtccgggga cgtacattgt tgcaccgccc gccgcccctt cttgattccg gaggctgcat
     6841 gtgttagcaa tgctactcgc aaacccggtc cgctctgagt aacccctgtg cgtcaggctg
     6901 tttcccggac gggaggaggg cgtgtcatgg ctaccacacg gcgggtctgg tctctcggat
     6961 tgatcgtggg attgctgggg ctcttcctcg cctccgtggg cgagacagcc cagttcaact
     7021 ggcagcagtt caagggcacg caattgcggg tcatgctcaa caagcacccc tggcagattg
     7081 ccatcgagcc gcatctgaaa gatttcgagg cgctgaccgg catgaagctc gtcgtcgagg
     7141 tgtacccgga agaccagttc cgggcgaaga cctcggtgga gctgcagtcc gggagcggca
     7201 gcatcgacgt cttcatgacc atgcctgcac aggagggatt gaagtacctg cgggccggct
     7261 ggtaccagcc catcgacgag tacctgaagg acgcctccat cacctcgcct gactacaact
     7321 ggaacgattt tctggaggtt ggccgctccg ccatgacggt ggatggccga atcatcgggc
     7381 cgcccattca actggagacc aaccagctca tgtacaggaa ggacgtgttc cagaagtaca
     7441 acgtcaaggt gcccacgacc ctggacgagc tggaggctgc cgccaaggcg ctgaacggga
     7501 aagcgatgac ggatgacggc caggcgggct tctggttcgt cgcccggggc aagcgcgccg
     7561 ccgccacgtc cgtcttctcc ggctacctcc acgccatggg ggccgcgtgg ctcggcccga
     7621 cccgtgagcc gatcttcaac tccgacgaag gcatcaaggc cttcgagctc tatggccggc
     7681 tgctgcggtt gtacggtcct ccgggctccg agaacaacca ctggtacgaa gcctcatcca
     7741 tcatggggca aagcaaggcg gccatgtaca cggagttcga ctccatctcg gccttcgtgg
     7801 aagatccgga gaaatccagg gtgaagggca agatgggcta tgtcatgttc ccaaagggtc
     7861 cgaatggcaa gccggggacg atggcaaccg tatgggggct cggcatcccc aagaacagcg
     7921 ggaatccgaa agctggctgg cttttcctgc aatgggccac cagcaaggag accgtgct
//
LOCUS       DBNGZC010000401         3920 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_328835, whole genome shotgun sequence.
ACCESSION   DBNGZC010000401 DBNGZC010000000
VERSION     DBNGZC010000401.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_328835"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="AC16G0_07110"
     CDS             complement(<1..820)
                     /locus_tag="AC16G0_07110"
                     /inference="COORDINATES: protein motif:HMM:NF015114.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR5099427.1"
                     /translation="MGIERLVAALGDLSRNREVMPLLILQGERAVPALAEVLLGPASS
                     IPEARCLAAEALGAIGGEAAVTALIQGLRVHDVRTLDPVLRLAEEAVRNRAAEQLGKI
                     GNRRAVGPLVYALAREGVREAMPALARFGEMRAIFHSVRRLEDPCDRAAAADALLLFG
                     RAAVPALVRTLDERRPSPTDEAPVSVERRAEAVNLLSILGDRSVVPVLRSCLEDPAPA
                     VQLEASLALVAHLSAEAPECALAIIAQGVRHPRPPVAMRCLDALVKAGDRCIPHL"
     gene            complement(871..1527)
                     /locus_tag="AC16G0_07115"
     CDS             complement(871..1527)
                     /locus_tag="AC16G0_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099428.1"
                     /translation="MAHTLTLARWITALDAPGCPVCRLMREADRDFLVHALREGKARA
                     EMYERIRKAGGFCEGHTRILRRLGSKSLGDRRSLARLSGWLLDDLTSGVAPHGACPAC
                     ETADEYERVSLAALRDILHPATGDATLRSRFQHGEGLCLNHFVVTASLIGEPEGLRVL
                     AEVQARAWAALSQPLKAYLRKHDYRFSHEPKTPAEERSWVEAVAVIGGTPTGVMDESE
                     "
     gene            complement(1530..2276)
                     /locus_tag="AC16G0_07120"
     CDS             complement(1530..2276)
                     /locus_tag="AC16G0_07120"
                     /inference="COORDINATES: protein motif:HMM:NF039337.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6062 family protein"
                     /protein_id="HIR5099429.1"
                     /translation="MLLVSELKQALRQSGCPLCRVIRNGDRHYLAVFLREGKDDGRML
                     LRLLGSWGLCARHAGLLISLEPVERGDGLGTGTLYDWLLDQARRLLEMYRQVLSTEGG
                     SKPLGWPRRQGLRHRVDRALRRLKRHESCPACEAQEQHAAHVVQSFIQALEPAAELPE
                     IPTMYLACEGLCLPHWRAVMHTRQSSAVHQLVETKQREVVAALKLALEAEVDCRVGDG
                     ASRNDGEREPAYARALAAVAGQTEWRPPEG"
     gene            complement(2352..3551)
                     /locus_tag="AC16G0_07125"
     CDS             complement(2352..3551)
                     /locus_tag="AC16G0_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099430.1"
                     /translation="MPSMTGPFAIGSDSEREREAVAAPESPRVREYFDLRDALSDPGC
                     PVCARVDRAGREALETLAAEASSTAPARKTGLGLRGLCSIHAWSLLQVSEKRIGPAEA
                     YEAFLRGRMAALDRTLARSKNAPPGSWSSWIGSWADTARTWMRLRRGRRRCPACRSAL
                     AVERRDLCLFPDLITDMEFARAFEASIGLCLPHLNLCVRLQPDHPDLPRLLEAHVLKA
                     KRLQADLQDFIRRVKAPFGGPTHEEQRAIWDRVLEWTAGKPGVFGPERELAGQPGVRE
                     VRLGRVPWTAVRRRPTPVIAEGEQARMGEVERLSLENAKLQRRLMEVNREWGEESARR
                     AAFQYQVHKLSEDVKVLELNLAGARGEAKAGDIQAKRLRDENEALQETVRRLGDGNSP
                     GTARLER"
     gene            complement(3553..>3920)
                     /locus_tag="AC16G0_07130"
     CDS             complement(3553..>3920)
                     /locus_tag="AC16G0_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099431.1"
                     /translation="EDEAAFQETLLQEVTHGLATGSLRDAFAASGGSCLPHLAILLHR
                     APDPGTARWLLRIALDQARRLTQELDTFEAETANRTRRYGSTADAPLRAMICWAGLRG
                     MVHEPEGRIRVPTPCGGKD"
BASE COUNT          648 a         1421 c         1239 g          612 t
ORIGIN      
        1 aaaggtgcgg gatgcaccgg tcgcccgctt taaccagggc gtccaagcac cgcatggcga
       61 ccgggggtct ggggtgacgg acaccctgcg cgatgatggc cagagcgcac tccggcgcct
      121 cggcggacag gtgagccacg agcgcgaggc tggcttcaag ctgcacggca ggggcgggat
      181 cttcgaggca cgagcgaaga acggggacca ccgagcggtc acctaggata ctcaggagat
      241 tcacggcctc ggcgcgtcgt tccacactga cgggagcctc gtcagtcgga gaggggcgac
      301 gctcgtccag cgtcctcacc agcgcaggca ccgctgccct cccgaatagg agcagcgcat
      361 ccgcggcggc ggcgcggtcg caggggtcct caagccggcg cacactgtgg aagatggccc
      421 gcatctcccc gaaacgggcg agggcgggca tcgcctcccg caccccctct cgcgccaggg
      481 catataccaa cggccccacc gcccgacggt tcccaatctt cccgagttgt tcggcggccc
      541 ggttccggac cgcttcttcg gccaggcgca ataccggatc cagtgtccgg acatcatgta
      601 ccctgagccc ctggatcagg gcggtgacgg ccgcctctcc cccgatcgca cccagcgctt
      661 ccgccgccaa gcagcgggcc tctgggatgg atgaggccgg gccaaggaga acctctgcca
      721 gggccgggac cgcccgttcg ccttgcagga tcagaagcgg catgacctca cgattgcggg
      781 aaaggtcacc gagcgccgcc accaggcgtt caatacccat caccattctc ccgaatcacc
      841 ggtcacgact tgaggcccgc ggccgtccag tcattccgat tcatccatta ctccggtcgg
      901 cgtcccgccg atgacggcca ccgcctccac ccacgaccgc tcctctgccg gggtcttggg
      961 ctcgtggctg aagcggtaat cgtgcttccg caggtacgcc ttcaggggtt gcgacaaggc
     1021 cgcccatgcg cgagcctgaa cctccgcgag gacccgcaga ccctcgggct ccccgatgag
     1081 cgatgcggtt accacgaagt ggttgaggca aagcccctca ccgtgctgga atcggctgcg
     1141 aagcgtggca tcgccggtgg ccggatgcag gatgtcgcgg agggccgcca gactcacccg
     1201 ctcgtattcg tccgcagtct cgcacgccgg acatgccccg tgtggagcga caccggacgt
     1261 gagatcgtcc agaagccacc cagacagacg cgccaggctc cgccgatcac ccagggactt
     1321 cgagcccagc cgccggagga tgcgcgtatg gccctcgcag aagccgccag ccttccggat
     1381 gcgctcatac atctccgcgc gagccttccc ctcgcgcagg gcgtggacca ggaagtcgcg
     1441 gtcggcttcc cgcatcaatc ggcagaccgg gcatccgggg gcatccagag ccgttatcca
     1501 tcgcgccagc gtcaaggtat gggccatcat catccctcgg gtggccgcca ctctgtctgg
     1561 cctgccacag ccgccagggc cctggcgtat gcaggctccc gttcgccatc gttgcgggac
     1621 gccccatccc cgactctgca atccacctca gcctccaagg ccagcttcag tgccgccacg
     1681 acctcacgct gctttgtctc gacgagctga tggaccgccg atgattgccg ggtgtgcatc
     1741 acggcccgcc agtgaggaag gcacaagccc tcgcacgcca gatacattgt cgggatttcg
     1801 ggcaactcgg ctgccggctc caatgcctga atgaacgact ggaccacgtg cgcggcatgt
     1861 tgctcctgcg cctcgcaggc ggggcatgac tcatggcgct tcaggcggcg gagggcgcga
     1921 tccaccctat gccgcaggcc ctggcgacgg ggccagccaa gcggtttgct gcccccttct
     1981 gtcgacagca cctggcggta catctccagc agccgccgcg cctgatccag gagccagtcg
     2041 tagagcgtcc cggtgcccag gccatcgccg cgctccaccg gctcaaggga gatgaggagg
     2101 cccgcatgcc gcgcgcagag accccaggag cccaggagcc gcagaagcat ccgcccgtca
     2161 tctttcccct cgcgcagaaa cacggcgagg taatgccggt ccccgttgcg gatgacccta
     2221 caaagcggac accctgactg ccgcaaggcc tgcttgagtt cgctgaccag gagcatgtcc
     2281 ggccgagcac gttcctgcaa gcgtggtgat cgtctcgctt gtgtctgtgg accggaggtg
     2341 cggaccatcc gtcagcgctc caatctcgcg gtcccgggtg agttgccgtc cccgagccgg
     2401 cgcaccgtct cctgaagggc ctcgttttcg tcccgcaggc gcttcgcctg gatatcgcca
     2461 gccttggcct cgcctcgtgc gcccgccagg ttcaattcaa gtactttcac gtcctcgctc
     2521 agcttgtgga cctggtattg gaaggcggcc cggcgcgcgc tctcctcgcc ccactcacgg
     2581 ttgacctcca tcaggcggcg ctggagcttg gcattctcca ggctcagccg ttccacctcg
     2641 cccatccggg cctgctcgcc ttcagcgatc acgggcgtgg gcctccgccg gaccgctgtc
     2701 caagggactc gacccaggcg gacttctcgg acacccggct gaccggcaag ctctcgttcg
     2761 gggccgaaca cgcccggctt gcctgccgtc cactccagca cccggtccca aatggcccgt
     2821 tgctcctcgt gggtcgggcc cccaaacggc gccttgaccc gtcgaatgaa gtcctgcaaa
     2881 tcggcctgga ggcgcttggc cttgaggacg tgcgcttcaa gcagtcgcgg caagtcgggg
     2941 tgatcgggct ggaggcgtac gcacaggttc agatggggaa ggcagaggcc gatcgaggcc
     3001 tcgaaggccc gcgcgaactc catgtccgtg atgaggtcgg gaaacaggca gaggtcacgc
     3061 cgttcgaccg ctagcgccga tcggcaggcc ggacatcgcc gccgcccgcg ccgtaggcgc
     3121 atccaggtcc gcgccgtatc ggcccacgac ccgatccagc ttgaccacga tccagggggg
     3181 gcattcttgg agcgggccaa tgtgcggtcg agggcggcca tccgaccacg caggaaagcc
     3241 tcgtaggcct ccgccggtcc gatgcgcttc tctgagacct gcaggagact ccaggcgtgg
     3301 atgctgcaga gaccccgcaa tcccaacccg gtctttcgcg cgggggccgt tgacgacgcc
     3361 tctgccgcca aagtctccag cgcctcgcgc ccggctcggt caacccgcgc acagacggga
     3421 cacccagggt cgctcagtgc atcccgcaga tcgaaatact cgcggacccg gggcgactcc
     3481 ggagcggcga cggcctcccg ctccctctcc gaatccgacc caatcgcgaa aggacccgtc
     3541 atgctcggca tctcagtcct tcccgccgca gggggtgggc acccggatgc ggccctccgg
     3601 ctcgtggacc attccccgta gccccgccca gcagatcatg gcccggagcg gggcatcggc
     3661 ggtcgatccg tatcgccgcg tgcgattcgc ggtctccgcc tcaaaggtat cgagctcctg
     3721 cgtcaaccgc cgagcctgat ccagcgcgat ccgcagcagc cacctcgccg tgccgggatc
     3781 cggggcccga tggagtagta tcgccaggtg tgggaggcaa ctcccgcccg acgccgcgaa
     3841 cgcgtcgcgg agagaccccg tggccaggcc gtgggtcacc tcctgaagca gggtctcctg
     3901 aaaggcggcc tcatcctcac
//
LOCUS       DBNGZC010000402         1587 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2381239, whole genome shotgun sequence.
ACCESSION   DBNGZC010000402 DBNGZC010000000
VERSION     DBNGZC010000402.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2381239"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="AC16G0_07135"
     CDS             <1..776
                     /locus_tag="AC16G0_07135"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR5099432.1"
                     /translation="HTITPAIPVWAGFGPATFGPTVDPKTGTPYTWTKDGFEATRYVL
                     ATDQLGTQLDPPAHWNPDYPAIDELPPTFAVRPLVVISIVDQVAKQPGYHLTVVDIQA
                     WEAVHGRIPEGSVVFIRSGWSKDWPNPDLSTRRPFPGITLAALQFLHQQRHILFHGHE
                     ALDTDTTATLEGEAWLLKNGYTQAEGVANLDQVPEAGALVEIGFPKFQGGTGGYARFI
                     AICPPDWKYGVAVGQLPESPLAKASSPLHWDAKVGMRVR"
     gene            907..980
                     /locus_tag="AC16G0_07140"
     tRNA            907..980
                     /locus_tag="AC16G0_07140"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:941..943,aa:Arg,seq:tct)
     gene            complement(1011..1367)
                     /locus_tag="AC16G0_07145"
     CDS             complement(1011..1367)
                     /locus_tag="AC16G0_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099433.1"
                     /translation="MPSLLRRRGGFPVHVWAVAAALLVGCGGATPIKTLLDDPQRFDG
                     KTVRIAGEVQGSIGALGFGAYQVNDGTGTLTIVSEGGGVPRQGAKVGVEGTFRSAYTI
                     GTQSVAVVVEQRRYTP"
BASE COUNT          279 a          531 c          486 g          291 t
ORIGIN      
        1 cgcacacgat cacgccggcg atcccggtgt gggcgggatt cggtcctgcg accttcggac
       61 ccacggtcga tccgaagacg gggactccgt acacttggac caaggacggg ttcgaggcca
      121 cgcgctacgt gctggccacc gaccagctcg gcacccaact cgacccaccg gcccattgga
      181 atcccgacta tcccgcgatc gacgagctgc cgccgacctt cgccgtccgc ccgctcgtgg
      241 tgatatccat cgtggaccag gtcgcgaagc agccgggtta ccacctgacg gttgtcgata
      301 tccaggcgtg ggaggcggtg cacggacgga tccccgaggg gtccgtggta ttcattcgct
      361 cgggctggtc gaaggactgg ccgaatcccg atctgtccac ccggcggccc ttcccgggga
      421 ttacgctggc cgcgctccag ttcctgcacc agcagcgcca catcctgttc cacgggcatg
      481 aagcgctcga caccgacacg acggcaacgc tcgaaggaga ggcctggctg ctcaaaaacg
      541 gctacaccca ggcggagggc gtggcgaatc tcgaccaggt gcccgaggca ggggcgctcg
      601 tggagatcgg gtttcccaaa ttccagggcg gaaccggcgg ctatgcgcgc ttcatcgcca
      661 tttgccctcc ggactggaag tacggcgttg cggtaggcca gttaccggaa agtcccctcg
      721 ccaaggcgtc gagccccctg cattgggacg cgaaagtcgg catgcgggtg cgatgacggc
      781 actgggcttt ctcgtaacgc tttgaataat tggcgcgtgc acataggttg gacgcgagct
      841 agacgctcgt ttgccgtgat tgtccttggc gtcttagcgt ctgggcgtct cggcgtccag
      901 tcttttgcgc ccgtagctca gttggacaga gcatcggcct tctaagccga gggtcggagg
      961 ttcgaatcct cccgggcgca ctcaccccgc atccgcattg tcgatcacgc ctagggagtg
     1021 tagcggcgct gctccactac cacggccaca ctctgggtcc cgatggtgta cgcggagcgg
     1081 aacgtaccct cgactcccac cttggctccc tgccgtggca ccccgccgcc ttcactcacg
     1141 atcgtcagcg ttccagtccc gtcattcacc tggtaggctc cgaagcccag ggcgcctatc
     1201 gagccttgca cctctcccgc aatccggacg gtcttgccat cgaaccgttg tgggtcatcg
     1261 agcagcgtct tgatcggcgt ggctcccccg caaccaacga ggagcgcggc tgcaacggcc
     1321 cacacgtgta cgggaaaccc gccccggcgt ctcagaagac ttggcatgat cggagttcct
     1381 cggatggaga acgtggtcct caagctgctg cccgtgacac ccacaatcaa taattccgct
     1441 cggcgtataa tgttccgtgt gccggaaacg cgcgacttcg taacgcagcg gtagcgacag
     1501 tcaccagcgg actccttcgg cataccatga agcggtcatg gcaaggcgcg gcacagggag
     1561 atcccgtgcc gcggttcctg ctattcc
//
LOCUS       DBNGZC010000403         2335 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2674240, whole genome shotgun sequence.
ACCESSION   DBNGZC010000403 DBNGZC010000000
VERSION     DBNGZC010000403.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2674240"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..639
                     /locus_tag="AC16G0_07150"
     CDS             196..639
                     /locus_tag="AC16G0_07150"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR5099434.1"
                     /translation="MKRRYPDGPIVAVGGIVVKDGSVLLIRRGKEPSYGLWSVPGGAV
                     DLGEGLRAATRREIREECGIEIDVTDVIEVLERVVRDSDDRVQYHYVLIDYLALWVSG
                     DPTPSSEVLELRWVSPDDFPQYQMTRGTADVIQKMLAAGKRAGVI"
     gene            839..1018
                     /gene="thiS"
                     /locus_tag="AC16G0_07155"
     CDS             839..1018
                     /gene="thiS"
                     /locus_tag="AC16G0_07155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HIR5099435.1"
                     /translation="MAVQVNEDIIKRDRYEEVSLKSGDTVEVLTIMAGGCAGPPVMGG
                     PGSSVLGQEVCHARR"
     gene            1005..1781
                     /locus_tag="AC16G0_07160"
     CDS             1005..1781
                     /locus_tag="AC16G0_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HIR5099436.1"
                     /translation="MRGGETLKFGGKEFHSRLLVGTGKYPDYHTMQKALEVSGTEIVT
                     VAVRRVNLDRTQESLLDYIDLKKYTLLQNTAGCYTAEDAVKYARLAREVGMPEMVKLE
                     VIGDQRTLFPDTEALLKATKTLVKEGFVVLPYTNDDPIMAKKLEDAGAAAVMPLGAPI
                     GSGLGIRNPHNILIILQTVQVPVIVDAGVGTASDAAVAMELGCDGVLMNTGIAGAKDP
                     VMMAEAMRKAVEAGRQAFLAGRIPKKLYATASTTMEGMIE"
     gene            1893..>2335
                     /locus_tag="AC16G0_07165"
     CDS             1893..>2335
                     /locus_tag="AC16G0_07165"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF014622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009229 - thiamine diphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR5099437.1"
                     /translation="MGQALTGGLRAVQLREKDLSTHDLCDLGDRLLRITRPAGATLLI
                     NDRVDVAMALDAEGVHLARRSLPPQSARDLLGPGKLLGISCHSLADVREAINGGVDFV
                     VLGPIYATPSKAAYGPPVTTALLEEARAACSLPLLAIGGIKAPQVP"
BASE COUNT          408 a          689 c          823 g          415 t
ORIGIN      
        1 tgcggtgacc cacctgcggt gggtgcccct cgctcgggcg ctcgttgcgg cgtaccagac
       61 gtacgcctca ctcggcccag cgctcgccgc cttgcatctg gacttttttg agcagcctgg
      121 gcgaaaatga ctttctcagc atcctgttag gggggcttcg aggctcgcgt ctcgcggctc
      181 tcggctcgga gaccgatgaa gcgacgctat ccggatgggc cgatcgtggc ggtgggcggc
      241 atcgtggtca aggacgggag cgtccttctc atccggcggg ggaaggagcc gagctatggc
      301 ctctggtccg tccccggcgg cgcggtggat ttgggggagg gcctgcgtgc ggccacccgg
      361 cgcgagatcc gcgaggaatg cggcatcgag atcgacgtta ccgacgtgat cgaggtgctg
      421 gagcgggtgg tgcgggattc ggacgatcgg gttcagtacc attacgtcct catagattat
      481 ctggcgctct gggtgagcgg agatcccacg ccgtcgtccg aagtcctgga gctgcgctgg
      541 gtgtcgccgg acgatttccc gcagtaccag atgacgcgcg gcacggccga cgtgatccag
      601 aagatgctgg ctgcgggcaa gagggccggg gtcatctgac caagagccga cagggacagg
      661 gatagcgaca gggacaggtg ctgggcaggc gacaggggct cgggctttgc tatcggatgt
      721 cgctttcggt catcggggtg atcttgtgga aatccggctg aacggcaaaa tgcgggaggt
      781 ggagcagggc atcaccatcc gccagctcct ggagagcctg aagctccatc ccttgtgagt
      841 ggcggtgcaa gtcaacgagg atatcatcaa gcgcgatcga tacgaggaag tcagcttgaa
      901 gtctggggac acggtcgagg tgctgacgat catggcgggt ggctgcgccg gcccgccggt
      961 gatgggtggt cctggatcga gtgttcttgg gcaggaggtg tgccatgcgc ggcggtgaga
     1021 cgttgaagtt cggcgggaaa gagttccatt cccgcctcct ggtggggacc ggcaagtacc
     1081 ccgactatca caccatgcaa aaggcgctgg aggtctccgg gacggagatc gtgactgtcg
     1141 ccgtgcgccg ggtgaacctg gaccggactc aggagtcgct gctggactac atcgacctca
     1201 agaagtacac cctgcttcag aacacggcgg gatgctacac ggcggaggac gccgtcaagt
     1261 acgccaggct cgcccgcgag gtcggcatgc ccgagatggt caagctggaa gtcatcggcg
     1321 accagcggac cctcttcccg gataccgagg cgcttctcaa ggccaccaag acactggtga
     1381 aggagggttt cgtcgtcctg ccctacacaa acgacgaccc catcatggcc aagaagctgg
     1441 aggatgccgg tgcggcggcg gtcatgcccc tgggggcacc catcggctcg ggcttgggga
     1501 tccgcaatcc ccacaacatc ctgatcatcc tccagacggt gcaggtcccg gtgatcgtgg
     1561 acgccggagt cgggacggcg tcggatgccg ccgtggccat ggaattaggc tgcgatggcg
     1621 tgctgatgaa caccggcatc gccggggcca aggatcccgt gatgatggcg gaggctatgc
     1681 gcaaggctgt ggaggccggt cgccaggcgt tcctggccgg acggataccc aagaagcttt
     1741 acgcgaccgc gtccacgacg atggagggca tgatcgagta gggaggatcc ccaattcgaa
     1801 tttcgaattt cgaatttcga aatcccaggg agtggggact cctcctggtc accgatcgcc
     1861 accagaccgg cgggcgggat ctggtggagg tggtgggtca ggccttgacg ggggggctcc
     1921 gcgcggtcca gctccgagaa aaggatctct cgacccacga tctgtgcgat ctcggcgatc
     1981 ggttgcttcg gatcacccgg ccggccggcg ccacgctcct catcaacgat cgtgtggacg
     2041 tcgccatggc cctggatgcg gagggggtcc atctcgcgcg acgaagcctg ccaccccaat
     2101 cggcccgcga cctgctcggg ccgggaaaac ttctgggcat ctcctgccat tctctggcgg
     2161 atgtccgcga ggccatcaac gggggggtgg acttcgtcgt cctgggtccc atctacgcga
     2221 ccccgtcaaa ggccgcttac gggccgccgg tgacgacagc gctgcttgaa gaggcgcggg
     2281 cggcctgctc gcttcccctc ctggcgatcg gtgggatcaa ggccccccag gttcc
//
LOCUS       DBNGZC010000404         2354 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1597481, whole genome shotgun sequence.
ACCESSION   DBNGZC010000404 DBNGZC010000000
VERSION     DBNGZC010000404.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1597481"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="AC16G0_07170"
     CDS             <1..1029
                     /locus_tag="AC16G0_07170"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HIR5099438.1"
                     /translation="EDNLLDSKWGTPEEGQPTASDARREKLQGLVRRSVLILPVNVPK
                     FVEKAYARGADAIQLDLEDSIPLAGKGAARAMVRDAMRQCAKGGADILVRINKPYALA
                     LQDLDACVWPGLDAIHFPKAESAREVATVDRLIGELEKAGGIPAGSVQLSIAVETALG
                     LHNALSIALASPRIVDIALGPEDYTLDIGVEPSKDGRELFYGKAKMIVVARLAGVQPL
                     GTMGSIADYRDLEGWAASIRQANQMGFLGAACIHPAQVPPLNTYFSPTAAEVASARKA
                     MASFEAVHSEGRASVGVDGKMVDIPVVERARRVIARATAIQAKEARVRATFAVLGEAP
                     PEGGLRYN"
     gene            1510..>2354
                     /locus_tag="AC16G0_07175"
     CDS             1510..>2354
                     /locus_tag="AC16G0_07175"
                     /inference="COORDINATES: protein motif:HMM:NF016292.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099439.1"
                     /translation="MKSGAAALIFTLALALLAVPLPSSGQPPAKLYRIGWLGITTGGY
                     ETNPRQCPIKGDPYWQAWIEGLRERGYIEGQNLVMECRCTEGREERAPALAVELVSLK
                     IDLLAVYGTVQVRAAKQATSALPILMVGVIDPVRRGLVASLARPGGNVTGLTHTAGVE
                     IVGKYLELLKEAVPKVSRVAVLRHLAATPGPFIYQGERDATARALGVALQPYGVRGPE
                     DFAGVITSMTKAGDEALLMEPDPFWEAHRQRVVDLVAESRLPAVYPWRNFVETGGLMS
                     YDVNRR"
BASE COUNT          434 a          746 c          736 g          438 t
ORIGIN      
        1 gaagacaacc tgttggacag caaatgggga acacctgaag agggacaacc tactgccagc
       61 gacgcgcgtc gggagaagct ccaaggactg gtgcgacgtt ctgtgctcat cctgcccgtc
      121 aacgttccga aattcgtgga gaaggcctac gcgcggggag ccgacgccat tcaactggat
      181 ctggaggaca gcattccatt ggccggcaag ggcgctgcgc gggccatggt ccgggatgcg
      241 atgcgccagt gcgccaaggg gggtgccgac atcctggtgc gcataaacaa gccctacgcc
      301 ctcgctcttc aggacctgga tgcctgtgtc tggcccgggc tcgatgccat ccattttccg
      361 aaggcggaat ccgcgcgtga agtcgcaaca gtggataggc tgattggtga gttggagaaa
      421 gccggaggca tccctgcggg aagcgtgcaa ctttccatcg ccgtcgagac ggccttgggg
      481 cttcacaacg ccctatccat tgccctcgcc agtcctcgta tcgtggatat cgcgctcggg
      541 cctgaggatt acaccttgga tatcggagtc gagcccagca aggacgggcg cgaactgttc
      601 tacggcaagg caaagatgat cgtcgttgcg cgtctcgccg gggtacagcc gctcggtacc
      661 atggggtcca ttgcggatta ccgggatctg gaggggtggg ccgcctctat ccgtcaagcc
      721 aatcagatgg gcttcctcgg cgctgcctgt atccatccgg cccaggttcc gccactgaac
      781 acatacttct cccccaccgc agcggaggtg gcttccgccc gcaaggccat ggcctccttc
      841 gaggccgtac actccgaggg acgcgcctcc gtgggtgttg acggaaagat ggtcgacatt
      901 cccgtggtgg agcgcgcgcg gcgggtcatc gcccgcgcga ccgccatcca ggccaaggag
      961 gccagggtgc gcgccacctt cgccgtcctg ggtgaggccc ctccggaggg aggcctgcgg
     1021 tataactgag gttggcgctg ccaaatgtgc cgtagctgtg gagctgcgga agacctcagt
     1081 cgccgatcgc catacaagcg caaatggaca gcttgtctct tgtggcccgc agcgtcgcac
     1141 aacttgcata catcggttat ctccttattg acacgcgcaa gacaccaggc gtaagctagc
     1201 aatcaagcct ccttgttcct tcccacctca cgggggctta ttccagccgc tttcctgcga
     1261 atgaacgggc ctctggccct tgaaaccgta tcctcttctg acccatcgct ttttcctcgt
     1321 ggctgagagg cgtaccatgc cagatgcagc cacctccgtc ttttcctcag cactgacaac
     1381 gagcctttct gggtacgcct ctacgcccat gacctcgatg ggagttgcac cactattcac
     1441 ccgcccgcct cgggtggtcc gattagccgc accgatctgt agtgtggtag gtcaagctgg
     1501 gaggattcca tgaaatctgg agcagccgcg ctaatcttca ccctcgctct cgccctcctt
     1561 gcggttccgc tcccatcctc cgggcaaccg ccagccaagc tctatcggat cgggtggctc
     1621 ggcatcacaa ccggtgggta tgaaaccaac ccccggcaat gcccaataaa gggcgaccca
     1681 tactggcagg catggatcga ggggttgcga gagcgggggt atatcgaggg ccagaacctc
     1741 gtcatggagt gccggtgtac ggaggggcgg gaggagcgcg cccccgccct cgcggtggag
     1801 ctggtgagcc tcaagatcga cctcctcgcg gtctacggga ccgtccaggt ccgggcggcc
     1861 aagcaggcca ccagcgcgct ccccatcctc atggtggggg tcatcgatcc cgtgaggcgc
     1921 ggactggtcg ccagcctcgc ccggccaggc gggaacgtca cggggctgac gcacacagcc
     1981 ggtgtggaga tcgtggggaa atatctggag cttctaaagg aggctgtccc gaaggtctcc
     2041 cgcgtggcag tcctccgtca tttggccgcg acgccgggac ccttcattta ccagggggaa
     2101 agggatgcaa cggcccgcgc gctcggcgtg gcgctccaac cctatggagt tcgtggaccc
     2161 gaggattttg caggcgtcat cacctcaatg accaaggccg gggatgaggc acttctcatg
     2221 gagcccgacc ccttctggga ggcccaccga caacgggtcg tcgacctcgt cgccgagagc
     2281 cggctgccag ccgtgtaccc atggaggaac tttgtcgaga ccgggggact catgtcctac
     2341 gacgtgaacc gacg
//
LOCUS       DBNGZC010000405         2484 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2381518, whole genome shotgun sequence.
ACCESSION   DBNGZC010000405 DBNGZC010000000
VERSION     DBNGZC010000405.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2381518"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="AC16G0_07180"
     CDS             <1..460
                     /locus_tag="AC16G0_07180"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099440.1"
                     /translation="GTYASPLSMAATEVAERNQTIYWEMGGISDPIVQRGFKYLFRIT
                     PMGSTYGAKAAEYSKEVLAPKFGVAPDKLKVSIVHEVALYGTTVATGAEKRAREIGLN
                     VLGRDPYSARATDLSPLVLKLKAAQPDVLIATSYIQDLLIFWKQARELGF"
     gene            470..952
                     /locus_tag="AC16G0_07185"
     CDS             470..952
                     /locus_tag="AC16G0_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099441.1"
                     /translation="MIGTGAGYALTEFAAAMGDDATGVFNVDNTQYLINPSFAPGAKE
                     AAQAYQDIFKEPPRSGHSLMNYMGSQVLFDVISRAGSTDPEAIRKAALETDIPDQKTP
                     MGYGVKFAPPGDPIAGQDIRAFPMIYQWQKGKQLTVWPKGAAVAEAIIPWPSWQKHKA
                     "
     gene            1104..1988
                     /locus_tag="AC16G0_07190"
     CDS             1104..1988
                     /locus_tag="AC16G0_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5099442.1"
                     /translation="MGVILAQVAVSGILLGGVYALISIGLTLIFGVLRVVNFAHGEFL
                     MIAMYASFWLFQLWGVGPYVSMPVVVALLFVVGVVVQFLVIRPILKASASMQIAATVG
                     LSTLLQNLALVIMTANYRSVDTPLSKAVVSAGPILLGVSRLLAFLVALAITGLLFLFL
                     QRTYPGKAIRCTTQDRTAALLMGINIDRVYLITFAIGSALVGVAGALLMPLYPVFPTV
                     GAYFVLVAFVVVVLGGLGNLTGALLGGFIIGLVEAFSGFYVPELKEAVYFVVFIIVLL
                     IRPTGLFGVVGAEEVGLK"
     gene            1985..>2484
                     /locus_tag="AC16G0_07195"
     CDS             1985..>2484
                     /locus_tag="AC16G0_07195"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5099443.1"
                     /translation="MSAPTRSQGSMLSALRFIPRRSLFVLAGVAVIAAIVAFTVTDKF
                     YLHLLIMMLFYAAVSSAWNIVGGFAGQLSLGHAAFFGIGAYTSTLMFIDLGISPWIGM
                     LIGAALATLISGAIGYPSFRLRGPFFTLVTIAFAEVLRILTIYAHDFTKGSIGISVPF
                     RPAVGN"
BASE COUNT          392 a          822 c          760 g          510 t
ORIGIN      
        1 cggcacctac gcgagccccc tgtccatggc ggccaccgag gtcgcggagc ggaatcagac
       61 gatctattgg gagatgggcg gaatttcgga tcccatcgtc cagcgcggtt tcaagtatct
      121 cttccgcatc acccccatgg gctccacgta cggcgccaag gcggcggaat acagcaagga
      181 ggtcctcgcg cccaagttcg gcgtcgcgcc ggacaagctg aaagtgtcga tcgtgcacga
      241 agtcgcgctc tacgggacga ccgtggccac cggggccgag aagcgcgcca gggagatcgg
      301 cctgaatgtc ctgggtcggg atccgtacag tgccagggcc accgatctct cccccctggt
      361 gttgaagctg aaggcggccc aacctgacgt gttgatcgcc acctcctaca tccaggacct
      421 gctgatcttc tggaagcagg ccagggagct gggattctga cccaaagcgg tgatcgggac
      481 gggtgccggc tacgccctga cagagttcgc cgctgccatg ggcgatgacg ccacgggcgt
      541 gttcaacgtg gacaacaccc agtacctgat caatcccagc ttcgccccgg gcgccaagga
      601 ggctgcgcag gcgtaccagg acatcttcaa ggagccgccc cgctccggcc attcgctcat
      661 gaactacatg ggctcccagg tcctgttcga tgtgatctcc cgggccggat ccaccgaccc
      721 ggaggcgatc cgcaaggcgg ccctggaaac cgacatcccg gatcagaaga cgccgatggg
      781 ctacggggtg aagtttgccc ctcccggcga cccgattgcg ggacaagaca tccgagcatt
      841 cccgatgatc taccagtggc agaagggcaa gcagttgacc gtctggccca agggggctgc
      901 ggtggctgag gcgatcatcc cctggccctc gtggcagaag cacaaggcat gaggcagata
      961 cggaggatgg agggccgctc gtcgaagacc aagacaaccg ggtgatggag gggccgccct
     1021 cgctgatcgc ggcgtgggcg gccttcctgt atccatcgac cccgttgtag tgatacacag
     1081 cctgtcctga gaaaaggtgc gacatgggag tcatccttgc gcaggtggcg gtgtccggca
     1141 tcctcttggg aggcgtgtac gccctgatta gcatcggtct gaccctcatc tttggagttc
     1201 tgcgggtcgt caacttcgcc catggcgagt ttctcatgat tgccatgtac gcctccttct
     1261 ggctgttcca actctgggga gtcggtccct acgtctccat gccggtggtc gtggccctgc
     1321 tcttcgtggt cggcgtcgtt gtccagttcc tggtgatccg ccccatcctg aaggcctccg
     1381 cctccatgca gatcgccgcc accgtcggcc tctccaccct gctgcagaac ctggcactcg
     1441 tcatcatgac ggccaactat cggtccgtgg acacccccct ttccaaggcg gtcgtgtccg
     1501 cgggtcccat actgctcggg gtgtcgcggc tgctcgcgtt cctggtggcc ctggccatca
     1561 cggggctcct cttcctcttc ctgcagcgga cctatccggg caaggcgatc cgctgcacga
     1621 cccaggatcg gaccgcggcg ctcctgatgg ggatcaacat cgaccgcgtc tacctgatca
     1681 ccttcgccat cggttccgcc ctggtgggcg tcgccggcgc gctactgatg ccgctctacc
     1741 ccgtgtttcc gacagtcggc gcctacttcg tgctggtggc attcgtggtg gtggtgctgg
     1801 gagggttggg gaacctcacc ggggccctgc tcgggggatt cataatcggt cttgtggagg
     1861 cgttcagcgg tttctatgtc ccggagctga aggaggccgt ctacttcgtg gtcttcatca
     1921 tcgtgctgct catacgcccg accgggctct tcggcgtcgt cggagccgag gaggtgggcc
     1981 tcaaatgagc gccccgactc ggagccaggg ctcgatgctc tccgccctcc gtttcatccc
     2041 ccgccgctcc ctgttcgtcc tggccggcgt cgccgtgatc gctgcgattg tcgccttcac
     2101 ggtgacggac aagttctacc tgcacctctt gatcatgatg ctgttctatg cggctgtcag
     2161 ttccgcctgg aacatcgtcg gaggctttgc gggacagctc tccttgggac atgccgcctt
     2221 cttcggcatc ggcgcctaca cctcgacact gatgttcatc gatctgggga tttccccctg
     2281 gatcgggatg ctgatcgggg ccgccctggc caccctgatt tcgggcgcca tcggctaccc
     2341 cagctttcgg ctccgcggcc ccttcttcac cctggtgacc atcgccttcg ccgaggtgct
     2401 ccggatcttg acaatctacg ctcacgattt caccaagggt tccatcggaa tcagcgtgcc
     2461 cttccggccc gccgtcggca actt
//
LOCUS       DBNGZC010000406         1711 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2185444, whole genome shotgun sequence.
ACCESSION   DBNGZC010000406 DBNGZC010000000
VERSION     DBNGZC010000406.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2185444"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="AC16G0_07200"
     CDS             <1..451
                     /locus_tag="AC16G0_07200"
                     /inference="COORDINATES: protein motif:HMM:NF014841.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5099444.1"
                     /translation="EFGMIPSSLDDLLISSQMVFVLAGATAENRAMLDRRKLNLMRKG
                     ACLVLVSRASLVDFDALSAKLATGDIRAAVDVFPEEPFSRHHALRKLDNVILSAHRSG
                     SLASAYHLMGEMLVDDLTQILAGLPPVRLQRAEPETVARFRSMPIKK"
     gene            505..687
                     /locus_tag="AC16G0_07205"
     CDS             505..687
                     /locus_tag="AC16G0_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099445.1"
                     /translation="MKRHGIGYVFLGRELGGKPRDPGFPIDDSARFAVIGKSAGFRTG
                     LDRLPSQATRIFQELC"
     gene            769..1539
                     /locus_tag="AC16G0_07210"
                     /pseudo
     CDS             769..1539
                     /locus_tag="AC16G0_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019879624.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
BASE COUNT          287 a          546 c          558 g          320 t
ORIGIN      
        1 ggagtttgga atgattccct cgtccctgga cgatctcctg atatcctcgc agatggtgtt
       61 cgtgcttgcc ggcgccacgg cagagaaccg ggccatgctg gatcggcgca agctgaacct
      121 gatgagaaag ggcgcttgct tggtgttggt gagccgggca tccctcgtgg acttcgacgc
      181 cctgagcgcg aagctggcca caggcgacat ccgtgcggcg gtggacgtgt ttccggagga
      241 accgttctcc aggcatcatg ctctccggaa gctcgacaac gttatcctct ccgcgcaccg
      301 atcggggagc ctcgcgtctg cgtaccacct catgggggag atgctggtgg acgatctgac
      361 gcagatcctg gccgggctac cgccggtccg gcttcaacgg gccgagccag agaccgtcgc
      421 tcgtttccgg agcatgccga tcaagaagta agcaccggct gaggcgaagc gaccaaccag
      481 caggcttcac cactccaggc aagaatgaag aggcacggca tcggttacgt gtttctgggc
      541 agggagctgg gcggcaagcc gcgcgaccct ggctttccca tagatgacag cgcccgcttt
      601 gccgtgatcg ggaagagtgc aggtttccgg acaggcctgg accgattgcc cagccaggcg
      661 acccgaatct tccaggaatt gtgttgacag tcatgtgacc gctggtgtag aggacaggcc
      721 ggcgtgaaga tgcccggcct gcccgcaaga ccagcgagga cgcccgtgat ggaccatccc
      781 cggaaactgc gacagcagat ccgcgacggg aagtggcgga aaccgacggc cggcctggcg
      841 cccggttacg tccaggccaa cctcgtggtc ctgcctcgcg acctcgccta cgacttcctc
      901 ttgtttgccc agcggaaccc gaagccctgc ccgatcatcg aggtgacgga tgtcggctcg
      961 tccgagccac gactgtcggc gccgggggcg gatctgcgca ccgacgtccc cgggtaccgg
     1021 atctatcgtg aggggtacct ggccggagaa gtcacggatc tccttggcgt ctggacgcag
     1081 gactcggtgg cattcctact cggctgcagc ttcaccttcg agaccgccct gctccaggct
     1141 ggggttcctg tccggcacat cgaggagggg aagaacgtct ccatgttcat cagcacggtg
     1201 ccgtgccttc cggcaggggt atttcagggg ccgctggtcg tgaccatgcg accgattccg
     1261 gcacccctgg tggcccgtgc cgtgcagatc agcgggcgat ttcccggcgt ccatggagcg
     1321 cccgtgcaca tcggcgatcc cgccgccatc gggatccggg atctggggag accggacttc
     1381 ggcgatccgg tgacgatccg tcccggcgag atcccggtat tctgggcctg cggcgtaacg
     1441 ccgcaggccg tggcgatgca ggtgaagcca gcgctgatgc tcacccacgc ccccggccac
     1501 atgttcatca ccgacctccg tgacgagtcg ctggcagtct tctatcggcc ttgctcgcgc
     1561 ccccgtcctg gcttgtctgg cgtgtatcat ttgacgggtt ttcccttgac atcgcgcttg
     1621 ccggctgggg tatgcttgga tccacaggaa tcggcgtggg tggcccacaa cctgagtctt
     1681 gtcacgtcac cgagcggccg tatcatgcgg a
//
LOCUS       DBNGZC010000407         2864 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2185523, whole genome shotgun sequence.
ACCESSION   DBNGZC010000407 DBNGZC010000000
VERSION     DBNGZC010000407.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2185523"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /locus_tag="AC16G0_07215"
     CDS             complement(<1..1050)
                     /locus_tag="AC16G0_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099446.1"
                     /translation="MPNTLPQHVVFIAGGAVAGSEAAFQLARHGVLCVVLEQNDRPYG
                     KIEDGLPRWHVNLRRQEAKKIDEKLAHPGVRFVPRTKLDRDLGLAEILAWGPSAVVLA
                     TGAWCDRPLPIPDVDRFVGRGLFYQNPLVYWFNHYPEPGYRGPQVAIADQALVVGGGL
                     ASLDVVKIVMLETVSRALAERGHDLHLYELERLGIARVLGELGLTLLSLQLQGCTLIY
                     RRQVDDMPLAEVAIDATPDQLERARATRRRLLQNFADKYLFAFRDRRVPVGYLAEGDR
                     LAGLTLATTEIKDGRAIPLGGSEHDVRSPMVISSIGSIPAPIRGIWMQGSVYRVRDAR
                     SGELEGLEGVLAVGNA"
     gene            complement(1759..>2864)
                     /locus_tag="AC16G0_07220"
     CDS             complement(1759..>2864)
                     /locus_tag="AC16G0_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR5099447.1"
                     /translation="MGNSASLTGRYLARELQIPVPRTRRPHTGQTLTIVGAREHNLKN
                     IEVEIPLGLFTCVTGVSGSGKSTLVTDILRKALDARLYGGRERPGEHDKILGIEHIDK
                     VIDIDQAPIGRTPRSNPATYTGVFSFIRELYALTPEARMRGYRPGRFSFNVKGGRCEA
                     CQGDGLIQIEMHFLPDVYVTCDVCKGKRYNRETLEIKFKGANIAEVLGMTVEEALRFF
                     ENVPRIKEKLQTLYDVGLGYIKLGQSATTLSGGEAQRVKLSRELSRRGTGRTLYILDE
                     PTTGLHFADIQQLLDVLHRLRDAGNTVLVIEHNLDVVKTADHLIDLGPEGGDAGGQII
                     ATGTPEEVARIKGSYTGQFLRRILKNGKGRIER"
BASE COUNT          510 a          922 c          896 g          536 t
ORIGIN      
        1 cgcattcccg acggcgagca cgccctccag gccttccaat tcgcccgatc gcgcgtctct
       61 tacccgatac acgctcccct gcatccagat cccccggatg ggtgccggga tacttccgat
      121 ggaactgatc accatcggag accgcacatc gtgctcgctc cctcccagcg ggatggcccg
      181 accatccttg atctccgtcg tcgccagggt gagacccgcc aaacgatccc cctctgcaag
      241 gtaccccaca ggcacccgcc ggtcccgaaa agcgaaaagg tacttgtccg cgaagttctg
      301 gaggagtctc cgacgcgttg cgcgtgctct ctccagttgg tccggtgtgg catcaatcgc
      361 gacctctgcg agcggcatgt cgtccacctg gcgacgatag atgagcgtgc atccctggag
      421 ctggaggctc aggagcgtca gcccgagttc acccagcacc ctcgcgatcc ccagacgctc
      481 cagttcgtag agatgcaaat catgcccccg ctccgccaag gcgcggctga ctgtttccag
      541 catcacgatc ttgaccacat ccaacgaggc gagaccgcct ccgacgacca gggcttggtc
      601 cgcgattgcc acctgcggac cacggtaccc cggctccggg tagtggttga accagtatac
      661 cagggggttc tggtagaaga gaccgcggcc gacgaaccga tcgacgtcgg gaatcggcag
      721 tgggcgatcg caccaggcac ccgtggccag gaccacggcg ctcggccccc aggcgaggat
      781 ctcggcgagg cccagatcac ggtccagctt cgttcggggg acgaatcgca cgccaggatg
      841 ggccagcttc tcatcgatct ttttcgcttc ctggcggcgg agatttacgt gccagcgggg
      901 gaggccgtcc tcgattttcc cgtacgggcg gtcgttctgc tccaagacga cacaaagtac
      961 tccatgccgg gcgagctgga aggccgcctc ggagccagcc acggcacccc cggcgatgaa
     1021 gacgacgtgt tgcggcagag tattgggcat ggctcgtatc cggcgcgaaa cgggtagtct
     1081 gtcccaggac atttcctggc ttgaaggtac gcgacgggcg agcgggcagt caaggaatat
     1141 ggagcgaggg gacgtcttga ccccgcaagg ggctcggcgc agacgcaggg gtccgatgcc
     1201 gacacctcac cgagtcatga ggcgccgggc ggacaggcta ccgccggaga gcggtggtca
     1261 acactcggct gattgaagaa aaaaatgccc ccggctcgat tgctcgaacc gggggcctgg
     1321 tcgcgggacc ggggaggggg ggagtcggtc ccacggcaac agcgaatgaa tcccaggact
     1381 ggccaatgac tggtggtcgg gttgcctggg tatgaagccg gccctcatat cgttgccggg
     1441 caacctttac tgaggcaatc cgcatgccac cgagtctgtc ccctagctaa tttcctcgta
     1501 acgagactca gcgccgtaac ccctcatatt tccgttggtt ccggagaatc aagagagttt
     1561 ccggagaatt cggaccattc aggtattgct gcttcggtgg gtcagtttgc ccctggtcgt
     1621 ccatctcaat gggacgatca ggaccatggt gactccgatg gtgaggaaga cgaaagttgg
     1681 aaggaagtga aacgagccag ccgcatcggg ctttcttttc gggtagacag aatggaagac
     1741 ccggttgtga gccgtggatt atcgctcaat cctgcccttt ccgttcttga ggatccgacg
     1801 caggaactgg cccgtgtatg atcccttgat ccgggcaact tcctcgggtg ttccggtggc
     1861 gatgatttgg ccgcctgcgt cacccccctc cggcccaagg tcgatcaggt ggtcggcggt
     1921 cttgaccaca tccaggttgt gctcgatgac cagcaccgtg ttccctgcgt cccggaggcg
     1981 gtgcagcacg tcgaggagct gctggatgtc ggcgaagtgg aggccagtcg tgggttcgtc
     2041 cagaatgtag agggtccgtc ccgtgccccg ccgggagagt tcccggctga gctttacgcg
     2101 ttgcgcctcc ccaccggaca gcgtcgtggc cgactgcccc agcttgatgt aacccaggcc
     2161 gacatcgtac agcgtctgga gcttctcctt gatccgcggc acgttctcga agaaccgcag
     2221 ggcctcctcc actgtcatgc ccaggacctc ggcgatgttt gcccccttga acttgatctc
     2281 cagcgtctcc cggttgtagc gcttcccctt gcacacatcg caggtcacgt agacatcagg
     2341 caggaagtgc atctcgatct ggatcaggcc gtctccctga caggcctcgc agcgaccccc
     2401 cttcacgttg aagctgaacc ggcccggacg gtacccccgc atgcgggcct cgggggtgag
     2461 ggcgtagagc tcccggatga agctgaacac gcccgtgtac gtcgccgggt tgctccgagg
     2521 cgtccggccg atgggtgcct ggtcgatgtc gatcaccttg tcgatgtgct cgatgcccag
     2581 gatcttgtcg tgctcccccg gccgctcccg cccgccatac agcctggcat ccagggcctt
     2641 ccgcaggatg tcggtcacca gcgtactctt gcccgaaccg gacacccccg tgacgcaggt
     2701 gaagagcccc aaggggatct ccacctcgat gttcttcagg ttgtgctccc gcgcccccac
     2761 gatggtgaga gtctggcccg tgtgcgggcg gcgggtgcgc ggcacgggga tctggagctc
     2821 ccgggccagg taccgcccgg tgagggaggc ggaattcccc atga
//
LOCUS       DBNGZC010000408         2455 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_425242, whole genome shotgun sequence.
ACCESSION   DBNGZC010000408 DBNGZC010000000
VERSION     DBNGZC010000408.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_425242"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..983
                     /locus_tag="AC16G0_07225"
     CDS             <1..983
                     /locus_tag="AC16G0_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR5099448.1"
                     /translation="SLETVQVRTLTVGEALIRVKAAGVCYTDTHFLSGVLNLGIQPLT
                     LGHEIAGEVAELGREARGVAVGDRVLVYYYATCGHCHWCRTDRANLCPQVVAEYGFTA
                     DGGYAEYVVVPAANLVKIPAGLDLKEAATLGCSVTTAIHAARTIADLRLGETVVVYGA
                     GGVGFGLIQFCKLAGARVIAVGRSAAKRAKAAELGADHVVDAARDVAKEIGALTGGEG
                     ADVVFELVGTKASMTHSVSALRRRGRLVLIGYSEDSLSVHPLQLVIGELAVTASVGNT
                     YDELLQAVDLAAAGKIKAVVGRTVSLDQLPGTLEALRRGEIVGRAVVVFS"
     gene            1021..2133
                     /locus_tag="AC16G0_07230"
     CDS             1021..2133
                     /locus_tag="AC16G0_07230"
                     /inference="COORDINATES: protein motif:HMM:NF016338.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxE/PaaK family acyltransferase"
                     /protein_id="HIR5099449.1"
                     /translation="MAVEKIEDLRAAILRMIRRGADQPLDDAAFSELALRLFAYQFEE
                     NAAYRKFCQRRRKIPENLRNWLEVPPVPIAAFKELSLASRPLEEAVACFMTSGTTNPE
                     KRGKHYHFTLEVYDASATTFFKANVLPDVDRMRILILGSPPDLMPHSSLSHYLDLMLR
                     YFGSSGSRFYIGADGLKADDLTADLRAAERSGEPVCLLGASYAFVHFLDRCRADSLRY
                     RLPPGSRIMDTGGFKGQSREVPQTELYADFAETFSVPLDSCVNMYGMTELSMQCYDSP
                     VRRRALALPEERWMQAPPWARTVVLDADSLAPVSPGVRGIICHYDLANCSSVMGILTE
                     DVGIATAAGFRLLGRVRGAESRGCSVSVDEMLAAAG"
     gene            2142..>2455
                     /locus_tag="AC16G0_07235"
     CDS             2142..>2455
                     /locus_tag="AC16G0_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HIR5099450.1"
                     /translation="MTWRDESPWFLPGDVAAGGSVSTLMLTDTAARIWAARNAYLARL
                     PVAEVVEIVDHVVSLWLEPESPWLKAAALRIAAATPYGQAMVRTGLTRLMAGCRKEAL
                     LSL"
BASE COUNT          393 a          743 c          883 g          436 t
ORIGIN      
        1 tgagtctgga gacagtacag gttcgcaccc tgacggtggg tgaggcgctg atccgggtga
       61 aggccgcggg ggtatgctat accgacacgc atttcctgag cggggtcctg aacctgggga
      121 tccagccact caccctgggc cacgagatcg cgggcgaggt ggcagagctc gggagagagg
      181 cccggggggt tgcggtcggc gatcgagttc tcgtgtatta ctatgccacc tgtggccact
      241 gtcactggtg ccgcacagac cgagcgaacc tttgtccgca ggttgtggcg gagtacgggt
      301 tcacggccga cggcggctac gccgagtacg tggtcgtgcc cgcggcaaac ctcgtcaaga
      361 ttcccgccgg cttggacctc aaagaagcgg cgacactcgg gtgcagtgtc accacagcca
      421 tccacgcggc gcggacgatc gccgacctgc ggctgggcga gaccgtcgtg gtgtacgggg
      481 ccggcggcgt cggcttcggt ctgatccagt tctgcaagct ggcgggcgcc cgggtgatcg
      541 cggtcggtcg gtcggccgcc aagcgcgcga aggcggcgga gctgggggcc gaccatgtgg
      601 tggacgccgc acgggatgtc gcaaaagaga tcggtgccct cacgggaggc gagggagcgg
      661 acgtggtgtt cgagctggtg gggacgaagg ccagcatgac gcactcggtg agcgccctgc
      721 ggcggcgcgg cagattggtc ctgatcggct actccgagga ctcgctcagc gtgcatccgt
      781 tgcagctcgt gatcggggag ctggctgtga cggcgtcggt gggcaatacc tacgacgagc
      841 tgctccaggc ggtggatctg gcggcggccg gaaagatcaa ggctgtggtg ggtcgaacgg
      901 tctccctgga tcaattgccc ggcaccctgg aggccttgcg gcggggagag atcgtgggcc
      961 gggcggtcgt cgtcttttcc tgagccgaag agcgcaggag ccgcgcagcg ggggacaacg
     1021 atggcggttg agaagattga agatctcagg gcggcgatcc tccggatgat ccgaaggggc
     1081 gccgatcaac ccctggacga tgccgcattc agcgaactgg cgctccgcct gttcgcctac
     1141 cagttcgagg agaacgccgc ctatcgcaag ttctgccagc gacgccggaa gatccccgag
     1201 aacctgagaa actggctgga ggttccgcct gtcccgattg ccgccttcaa ggagctgagt
     1261 ctggcctcca ggccgctcga agaggctgtc gcctgcttca tgacgagcgg gacgacaaac
     1321 cctgaaaagc ggggcaagca ctaccatttc accttggaag tctacgacgc ctccgccacg
     1381 acgttcttca aggcgaacgt gctcccggat gtggaccgca tgcggattct gatcctggga
     1441 agcccgcccg acctgatgcc gcattcctcg ctctcccact atttggatct catgctccgc
     1501 tactttggat cgtccggcag ccggttctac atcggggcag acggcctgaa ggcggatgat
     1561 ctgacggccg acctccgcgc cgcagagcgg tccggggagc cggtctgcct tctgggggcg
     1621 agctatgcct tcgtccactt cctcgaccgc tgccgagccg atagcctccg gtaccgcctg
     1681 ccgcccggca gccggatcat ggatacgggg ggattcaagg ggcagtccag ggaggttccc
     1741 cagacggagc tgtatgcaga ctttgcggag acattctccg ttccgcttga ttcgtgcgtc
     1801 aacatgtacg gcatgaccga gctgagcatg cagtgctacg actcccccgt tcgccggcgg
     1861 gcgctggcac tgccggaaga gcgctggatg caggccccgc catgggcgcg gaccgttgtg
     1921 ctggatgcgg acagcctggc gccggtgtct ccaggggtgc ggggaatcat ctgtcattac
     1981 gatctggcga actgctcctc ggtcatgggc atcctgacgg aggatgtcgg aatcgccacg
     2041 gccgccggct tccgcctctt gggtcgggtc aggggggcgg agtcacgcgg ctgctcggtg
     2101 tcggtggatg aaatgctggc ggcggccggg tagggacctc catgacctgg cgtgacgaga
     2161 gcccgtggtt tctgccgggt gatgtggcgg cgggaggttc ggtctcgacg ctcatgctga
     2221 ccgataccgc agccaggatc tgggcggcgc ggaacgccta cctggcgcgg ctgcccgtgg
     2281 ccgaggtcgt ggagatcgtg gatcacgtcg tgagtctctg gctcgagccg gagtcgccct
     2341 ggttgaaggc cgctgccctt cgcatcgccg ccgccacgcc gtacggccag gccatggtgc
     2401 ggacgggtct cacgcgcctg atggccggct gccgcaagga ggcgttgctc agtct
//
LOCUS       DBNGZC010000409         2698 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_329417, whole genome shotgun sequence.
ACCESSION   DBNGZC010000409 DBNGZC010000000
VERSION     DBNGZC010000409.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_329417"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="AC16G0_07240"
     CDS             complement(<1..704)
                     /locus_tag="AC16G0_07240"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5099451.1"
                     /translation="MPRDLKTQVAVITGASSGIGRAAAVAFAREGVRVVLGARRIDHL
                     QAVVETIRASGGEARAIQTDVSQPAQVQRLVDEAAAAFGRLDILVNNAGLGYFGPLES
                     MAIQEARHLFEVNLMGTLLGIQAAVPIMRRQGSGHIINVASVLGKRATPGSGFYSATK
                     FAQVALSESLRLELADAGIQVSVVCPVSTMTEFFTVAASRSPLKIDPAGPTYTAEQVA
                     KVLVRCARRPRPEVMV"
     gene            complement(806..1378)
                     /locus_tag="AC16G0_07245"
     CDS             complement(806..1378)
                     /locus_tag="AC16G0_07245"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR5099452.1"
                     /translation="MAARLLAVADIHSRRGALRGIREEFARHAPDAVIVAGDLTNYGD
                     AAEVRRVLEDIPGRVLAIPGNLDLWPAFDAGIAGSHAESLVGAVIEIGSVVVAGLSAN
                     LPACDILVVHEPPQGILDDAGGGRHIGFPAHLEALHRLQPKVLLCGHCHESPGVTNHR
                     TTTVVNCTLGRGGHGALVEMDGDRVTARLL"
     gene            complement(1550..>2698)
                     /locus_tag="AC16G0_07250"
     CDS             complement(1550..>2698)
                     /locus_tag="AC16G0_07250"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012881950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR5099453.1"
                     /translation="LLVMDGLGGLPRSGDGKTEMEAARTPNMDALADTGSLGLADAVA
                     PGITPGSGPGHLGLFGYDPLQYRIGRGVLEAVGIDLTLTGRDLAARGNFCSVDAQGLI
                     ADRRAGRIPTEVCTRLCSLLEKIRIPGVELIVRPVREHRFVVLFRGQGLEEGLSETDP
                     QEVGKPPLPVQAEVPQAKHAAEIANRFVNEAKTALASEHPANMILLRGFSKHPSLPKL
                     TDIYGVKAAVIAVYPMYRGLARLAGMQAVVTGDTVADQITALESAFSQYDFFFVHVKK
                     SDSAGEDGNFDKKVTIIEEVDREIPRLLALRPDVLVVTGDHSTPAPFKAHSWHPVPVL
                     LWSKWCRRDHATRFSEQECRTGSLGHIRAAELLPIMMGYAERLTKFGA"
BASE COUNT          435 a          940 c          886 g          437 t
ORIGIN      
        1 tagaccatga cctcggggcg cgggcgccgg gcgcagcgca ccagcacctt cgccacctgc
       61 tcggcggtgt aggtcgggcc ggcgggatcg atcttgagcg gggatcgaga tgccgcgacc
      121 gtgaagaact cggtcatggt cgaaaccggg cacacgacgc tcacctggat gcccgcatcc
      181 gccagctcca atcggagtga ctcggacagg gccacctggg cgaacttggt ggcagagtag
      241 aatcccgagc caggcgtggc ccgcttcccg aggaccgagg ccacattgat gatgtgcccg
      301 gagccttgcc gccgcatgat gggcacggcg gcctgaattc cgagcagcgt gcccatgagg
      361 ttcacctcga agaggtgccg ggcttcctgg atcgccatgg actcgagcgg gccgaaatag
      421 ccgagaccgg cgttgttgac gaggatgtcc agtctgccga aggccgccgc cgcctcgtcg
      481 accaggcgct gcacttgggc gggctggctc acatcggtct gaatggcgcg cgcctcaccc
      541 ccggacgccc ggatggtttc cacgaccgcc tgcaagtgat cgatccgacg ggcccccagg
      601 acgacgcgca ccccctccct ggcaaaggcg acggctgcgg ctcgcccgat gccgctggag
      661 gcgccggtga tcacagcgac ctgcgtcttc aggtcacgcg gcatggtcgg gatgttcccg
      721 cctcggattc attctgcctc gaccttccgg atcgccagcg ccggggccgg gcgtccccga
      781 cggagggtgt ttcccgcccc gggggttaca ggagccgcgc ggtcacccgg tcgccgtcca
      841 tctccaccag ggcgccgtgg ccgccccgtc ccagagtgca attcaccacg gtcgtggtgc
      901 ggtggttcgt caccccgggc gattcgtggc aatggccgca gagcaggacc ttgggctgca
      961 gcctgtggag ggcctccagg tgggccggaa agccgatgtg acgccccccg ccggcgtcgt
     1021 ccaggatgcc ctgcggtggc tcgtggacca ccaggatgtc gcacgcggga aggttggcgg
     1081 acaggccggc gacgaccacg ctcccgatct cgatcaccgc cccgaccagg ctctcggcgt
     1141 ggctccccgc gatccccgcg tcgaaggccg gccagaggtc cagattgccg gggatcgcca
     1201 agacccgacc cggaatgtcc tccaacaccc gccggacctc cgcggcgtct ccgtaattcg
     1261 tcaggtcgcc ggccacgatg acggcatccg gcgcatgccg cgcgaattcc tcccggatgc
     1321 cccgaagcgc cccccttcga ctgtggatgt cagcaacggc cagcaaccgt gccgccattc
     1381 ctccccgacc tccctgatgc tgattgcatg tcactcaggg gaaccagagg gaatcacctt
     1441 gaaccgcaga ttacgcaggt ttcacagatt tctcggagac agcctctccg gaataatctg
     1501 cgtgatctgc gaaatctgcg gataagtatc gggtctccct ccgaatgcct caggcgccga
     1561 acttggtcag acgctcggcg taccccatca tgatcggcag caactcggcc gcccggatgt
     1621 ggcccaggct gccggtccgg cactcctgct cgctgaagcg cgtggcgtga tcccggcggc
     1681 accacttcga ccaaaggagc accggcaccg ggtgccagct gtgcgccttg aagggcgccg
     1741 gcgtggagtg gtcgcccgtg accaccagga cgtccggccg gagcgccagg aggcgcggga
     1801 tctcccggtc aacctcctcg atgatggtca ccttcttgtc gaagttccca tcctcacccg
     1861 cgctgtccga cttcttcacg tggacgaaga agaagtcgta ttgactgaag gccgattcca
     1921 gggcggtgat ctggtctgcg acggtatccc cggtcaccac cgcctgcatc cccgccagtc
     1981 tggccagacc ccggtacatg ggatagaccg cgatgaccgc cgccttcacg ccgtagatgt
     2041 cggtcagctt gggaaggctg gggtgcttgg agaagccgcg gagcagaatc atgttggctg
     2101 gatgctcgct ggccagcgcg gtctttgcct cgttgacgaa gcggttggcg atctcggccg
     2161 cgtgcttggc ctgggggacc tccgcctgga ctggcagcgg cggcttcccg acctcctgcg
     2221 ggtcggtctc cgaaagaccc tcttccaggc cctggccgcg gaacagaaca acgaagcgat
     2281 gctcgcgcac cgggcgcacg atcaattcca cgccggggat ccggatcttc tccagcaggc
     2341 tgcagaggcg tgtgcagacc tccgtcggga tgcgcccggc gcgtcggtcg gcgatcagac
     2401 cctgggcatc cacgctgcag aagttgcccc gggccgcgag gtcccgcccg gtcagcgtga
     2461 gatcgatgcc caccgcctcc aggacgccgc gtccgatgcg atactggagc gggtcgtagc
     2521 cgaagaggcc caggtggccc gggccgctgc ccggagtgat gccgggggcc accgcatcgg
     2581 ccagacccag agatcccgta tccgccaggg cgtccatatt gggtgtgcgc gccgcctcca
     2641 tttccgtctt gccgtcgccc gatcgaggca ggccgcccag accgtccatc accagcag
//
LOCUS       DBNGZC010000410         6260 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1107541, whole genome shotgun sequence.
ACCESSION   DBNGZC010000410 DBNGZC010000000
VERSION     DBNGZC010000410.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6260)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1107541"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="AC16G0_07255"
     CDS             complement(<1..640)
                     /locus_tag="AC16G0_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR5099454.1"
                     /translation="MTATDAEDRGRSTWVIAVTSAVRAAFGVIWGIDAYLTWQPAFAD
                     HYVGYLTNAAHGQPAWLHPWFALWLAAVTPVPGLFVWLTRRLETLIAVGLLFGLLRKW
                     TYVLGGLFSLLIWSTAEGFGGPYTIGASNLGPALVYVLVFIALIIFDRTQGRTPYSLD
                     YYLERRFPAWQGTAEWAPAALREQAPPRLPWPAQAVAILGIVVAFLFFFGTLE"
     gene            complement(692..2110)
                     /locus_tag="AC16G0_07260"
     CDS             complement(692..2110)
                     /locus_tag="AC16G0_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR5099455.1"
                     /translation="MLNDSASSTRQKALALNLESAAHGTFAEIGGGQEVARWFFQVGG
                     AAGTVAKTISAYDMAVSDALYGPAQRYVSRQRLEAMLDHEFDQLRERLGPTRGDTTTF
                     FAFADTVATRSYRHPENGRGWAGLRFQARPHEDPSEIIVHAHLFDSTAVHQQEALGVL
                     GVNLIHGAFFRRSEPADLIHSLMDELSRARVEIDMIKLAGPAFAGVDNRLMSLQLVEQ
                     GLTDAAMFTASGEVVQPSEVLHKQPILVERGSFRPITKLTLDLLERAQEQFLAEPAVQ
                     GRQPVVLAEMTLRSLIPGPEVGHPDFLDRANILGALGIDVLISRFEPYHQLAEYLAAY
                     TDQMIGIAVGLPSVRHMLDEKYYTDLPGGVLESMGRLFKRSVKMYVYPSRDPAIDRIW
                     SIEQAPFSPPWHHLRDLLIGIRHLEPTRGYNEAYLSIQTPDVLARIEKGDPSWESMVP
                     PRVAEIIQAKKLFGWQVARVGT"
     gene            complement(2271..2732)
                     /locus_tag="AC16G0_07265"
     CDS             complement(2271..2732)
                     /locus_tag="AC16G0_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR5099456.1"
                     /translation="MQPRIEYAKVAPEGLRAMRGLETYARQSGLDPLLLELVKVRASQ
                     MNGCAYCIDMHTKDARARGESEQRLYALSAWRETPFFTDRERAALEWTEAVTAISDGH
                     APDEVYERVQQHFSEKELVNLSLAIVAINGWNRLCIGFRSVPGSYQPTGHR"
     gene            complement(2911..3291)
                     /locus_tag="AC16G0_07270"
     CDS             complement(2911..3291)
                     /locus_tag="AC16G0_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HIR5099457.1"
                     /translation="MIQVKRVYDPVTRKDGARFLVERLWPRGMKKEALHMDGWTKDVS
                     PSDALRRWFSHDPAKWEEFCRRYFIELDSRPEAWRPLLDVARRGNTTLLYSAHDTQHN
                     NAEALKAYLEKHMGARGRTSRVRD"
     gene            complement(3746..4945)
                     /gene="odhB"
                     /locus_tag="AC16G0_07275"
     CDS             complement(3746..4945)
                     /gene="odhB"
                     /locus_tag="AC16G0_07275"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HIR5099458.1"
                     /translation="MPTNIIVPEMGESVVGATVARWLKQEGERVTAGESIVELETEKV
                     DLEVAAEQPGILVKIGRQEGEEVRVGDILGVIEEPTAGVAQAPAEVKEASKTPETPTR
                     TEGGNGGQAIPASTQSPHPTSVSASQDAVAGSSRPRTPEAQERVESKRTEPRTTPAVP
                     ATMPGPSRGEERVHMSRRRQTIARRLVEAQHAAAMLTTFNEIDMSAVMDVRVRRKDAF
                     KERHGVSLGLTSFFVKAAVGALKEFPRLNAEIQGEDMLLKHYYDIGIAVGASEGLVVP
                     VLRDADRLSFAEIEQAVKAFAQKAGDGSLSLEDLRGGTFTITNGGVFGSLLSTPILNP
                     PQVGILGLHKIEARPVVRDGNVVVRPMMYVALSYDHRIVDGREAVQFLVRVKELTEDP
                     ERLLLEG"
     gene            complement(4965..>6260)
                     /locus_tag="AC16G0_07280"
     CDS             complement(4965..>6260)
                     /locus_tag="AC16G0_07280"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase component E1 family
                     protein"
                     /protein_id="HIR5099459.1"
                     /translation="SLHPERDLAEPFPAAPPPGAARRVQTAVPAERLREMHAALHKLA
                     GDFTINPKLERAIRRHLRSLDDLDEPSIEWATAEELALASILADGIAIRMTGEDVERG
                     TFGQRHAVFHDVKTGKTFTPLQNFPQARASFEIHNSPLTETAVVGYEYGYDIQEPRRL
                     VIWEAQYGDFINAAQVMIDEFLVSARAKWGQTPSLVLLLPHGYEGQGPDHSSGRLERF
                     LGLAAETNMRIANCTTAAQYFHLLRRQASLLETDPLPLLVMTPKSLLRHPRAASALRE
                     LAEGQWQPVIDDREARHQPEQVRRLILCSGKVYVDLVTNPLREETPVIAIARVEQLYL
                     FPAGDLQALLEAYPRLEEVVWFQEEPENMGAWTFMQPRLGKLIAGRWPLRYIGRPPNS
                     SPAEGSSAWHVANQKALVERAYNLESHVLEDVVISSEWT"
BASE COUNT         1022 a         2061 c         2051 g         1126 t
ORIGIN      
        1 tctccagggt gccgaagaag aacaggaacg cgacaacgat ccccaggatg gcgaccgcct
       61 gggccggcca ggggaggcgt ggtggtgcct gctcccgcaa ggctgccggg gcccactcgg
      121 ccgtcccctg ccaggcgggg aagcggcgtt ccagatagta gtcaaggctg tacggggttc
      181 gcccctgggt ccggtcgaag atgatgagcg cgatgaacac cagcacgtag accagggccg
      241 gcccgaggtt cgacgccccg atggtgtaag gacccccgaa gccctcggcg gtggaccaga
      301 tcaggaggct gaaaagccca cccaggacgt aggtccactt ccgcaggagg ccgaagagca
      361 agccgacggc gatgagggtc tcgagtctgc gcgtgagcca gacgaagagg ccgggcacgg
      421 gggtcacggc agccagccag agggcgaacc aggggtgcag ccaggccggc tggccgtggg
      481 cggcgttcgt gaggtacccc acgtagtggt cggcgaaggc aggctgccag gtcaggtagg
      541 cgtcgatccc ccagatcacg ccgaaggcgg cccgcaccgc actcgtcacg gcgatgaccc
      601 acgtggagcg tccccgatcc tctgcatctg tggctgtcat ggtcaccccc tcgctgtcat
      661 acctggcaag ttcagcaccc cggaaccact cctaggtgcc cacgcgcgcg acctgccagc
      721 cgaagagctt cttggcctgg atgatctctg ccaccctggg cggcaccatg ctttcccacg
      781 aaggatcgcc cttctcgatt cgggccagga cgtcgggtgt ctgaatggag aggtaggcct
      841 cattgtaccc ccgcgtaggc tcgaggtgac ggatcccgat gaggaggtcg cgcaggtggt
      901 gccagggtgg cgagaacggc gcctgctcga tgctccagat ccggtcgatc gcgggatctc
      961 gggagggata gacgtacatc ttcacggacc gcttgaacag gcgtcccatc gattccagga
     1021 ctcccccggg caggtccgtg taatacttct catccaacat gtgtctcacg ctggggagac
     1081 ccacggcgat gccgatcatc tggtcggtgt aggccgccag gtactcggcc agttgatggt
     1141 atggctcgaa ccgcgagatg aggacgtcga tgcccagagc gcccaggatg ttcgcccggt
     1201 ccaggaagtc cggatggcca acctcgggcc ccgggatgag gctccgcaat gtcatctccg
     1261 cgagtacgac cggctgccgc ccctggacgg cgggctccgc caggaactgc tcctgtgcgc
     1321 gctcgagcag atccagggtg agcttggtga tcggccggaa gctcccacgc tccaccagga
     1381 tcggctgctt gtggaggacc tcggatggct gcacgacctc tccgctcgcc gtgaacatcg
     1441 cggcatcggt caggccctgc tccaccagct gaaggctcat gaggcgattg tccacgcccg
     1501 caaaggctgg ccctgccagc ttgatcatgt cgatctcgac ccgcgcgcgt gacaactcgt
     1561 ccatcagcga gtggatgagg tccgccggct ccgaacgccg gaagaaggct ccgtggatca
     1621 ggttgacccc gagcaccccc agggcctcct gctggtgcac ggcagtggaa tcgaacaggt
     1681 gggcgtgcac gatgatctca gaggggtcct cgtgcgggcg ggcctggaac cggagccctg
     1741 cccaaccgcg accgttctcg gggtgccggt agctccgcgt ggccacggtg tcggcgaagg
     1801 cgaagaaggt cgtggtgtcg cctcgcgtgg gtcccagacg ctcccgcaat tggtcaaact
     1861 catgatccag catggcctcc agacgctggc ggctgacgta ccgctgggcc gggccgtaga
     1921 gggcgtcgct caccgccatg tcgtaggcgg agatggtctt ggccacagtc cccgccgcgc
     1981 cgccgacctg gaaaaaccag cgggccacct cctggccgcc accgatctcg gcaaaggtgc
     2041 cgtgtgccgc tgattccagg ttgagggcca gcgccttctg ccgggtgctc gatgccgagt
     2101 cattcagcat gcagggactc cgcacatggg tgaagctgac tgccctggat cttcactccc
     2161 aactcccacg ctcccctcgc ccgaatcttg gatctccggg gacccatcct cgggggcctc
     2221 ccccggcccc gtcctttccc cgccaagggg aggaaatctt ccgcatcagc ctatcggtgt
     2281 ccggtcggct ggtagctccc cggcacggac cgaaagccga tgcagagccg gttccagccg
     2341 ttgatggcga cgatggccag gctcaggttg accagctctt tctcgctgaa gtgctgctgg
     2401 accctctcgt agacttcgtc gggggcatgc ccgtcgctga tggccgtgac cgcctcggtc
     2461 cactccaggg cggcccgctc ccggtcggtg aagaacgggg tctcgcgcca ggcgctgagg
     2521 gcgtacaggc gctgctcgct ctccccccgg gcgcgggcat ccttggtgtg catgtcgatg
     2581 cagtaggcgc agccgttcat ctgcgacgcc cgaaccttca ccagttccag cagaagggga
     2641 tccagaccac tctgccgggc gtaggtctcc agcccacgca tcgcccgcag cccctccggg
     2701 gcgactttcg cgtattcgat tcgcggttgc atcgtggtct cctgtctctg tgcccttggc
     2761 gtcttgccct gatgcggatc ccttgctcgg atcgggggtg cgattgactt cacctgaaat
     2821 gggatccgca tgccgagggg tggagtgaaa agttgagggg gaaaccgccc ggagcggaac
     2881 atgtggcagc cgggaatccg ctccttggcc tcaatcccgg actcgcgagg tccgtcctcg
     2941 tgcccccatg tgcttttcca ggtacgcctt cagcgcctcg gcgttgttgt gttgcgtgtc
     3001 gtgagcgctg tagagcagcg tggtgttgcc gcgtcgtgcg acatccagca agggccgcca
     3061 ggcctcgggc cggctgtcca gttcgatgaa gtagcggcgg cagaactcct cccacttcgc
     3121 cggatcgtga ctgaaccagc ggcgcaaggc gtcgcttggg ctgacgtcct ttgtccagcc
     3181 atccatgtgg agggcctctt tcttcatgcc ccgcggccat aaacgctcga ccaagaagcg
     3241 cgccccgtcc ttccgagtca cgggatcgta cacgcgtttg acttggatca tgcaggtccc
     3301 tccagcatcg ttcgcacaca acgacggttc aggcgaccgc ccgcctcggt gtgctctcaa
     3361 tgggataggc cagttcagtc ctttcgctcc catcctgggt gtctcggccg tagccatgtt
     3421 catcctttca cttgcgtcag tacccaaccc ctcccggctg atcaacaaac gaagcgtgat
     3481 ccggcaaggt ctttggcgac ccgagccgga ttctcctccc ctcgacacgc acctcccgat
     3541 gcaaccaggc aggagaactc cgcaagccgt gccggacacc atccactact gagtatgctc
     3601 ttgggatggc gaatggtctg tgaaggcagt cacagaaggt ggagagggtg aaacctgagt
     3661 cggagtgggg gaaatggaaa cgcaggatcc ctggctctac cagaatcgaa attgcccctg
     3721 cgttctgttc tccggcaggc cggattcagc cctccagcaa gaggcgctcc ggatcttcgg
     3781 tcaactcctt cacccggacc aggaactgca ccgcttcccg tccgtccacg atccggtgat
     3841 cgtagctcag ggcgacgtac atcatgggcc ggacgaccac attgccgtca cgaaccacgg
     3901 gtcgtgcttc gatcttgtgc aatcccagaa tgccgacctg gggggggttg aggataggcg
     3961 tgctgaggag cgagccgaag acaccaccat ttgtgatggt gaaggtcccg ccgcgcaagt
     4021 cctccaggga gagacttccg tccccggcct tttgggcgaa ggccttgacc gcctgctcga
     4081 tctcggcgaa ggacaggcga tcggcatccc ggagcaccgg gaccaccagc ccctcggatg
     4141 cacccacggc gataccgatg tcgtagtagt gtttgaggag catgtcctcg ccctggatct
     4201 cggcattgag gcgggggaat tccttcaggg cccccacggc ggccttgacg aagaaggagg
     4261 tgaggcccag actcacacca tggcgttcct tgaaggcatc cttgcgccgg acgcgaacgt
     4321 ccatcacggc gctcatgtcg atttcattga aggtggtcag catggccgcc gcgtgctgcg
     4381 cttccaccag tcggcgggcg atggtctggc ggcggcggga catatggacc cgttcctcgc
     4441 cccgggaagg cccgggcatc gtcgcgggaa cggcaggggt ggtccttggc tcggtcctct
     4501 tcgactccac tcgctcctga gcctctgggg tcctcggcct gctggaaccg gccacggcat
     4561 cttgcgacgc gctcacgctg gtcggatgag gtgattgcgt cgacgcggga atcgcctgtc
     4621 cgccgttgcc cccttcggtg cgggtgggag tctccggcgt cttcgaagct tccttgacct
     4681 ctgccggtgc ctgcgccacg cctgccgtag gctcctcaat cacgcccaag atgtcgccca
     4741 cacggacttc ttccccttcc tgccggccga tcttcaccag gatgcctggc tgctcggccg
     4801 ccacctccag atccaccttc tcggtttcca gctccacgat cgactcgccg gcggtcacac
     4861 gctcaccttc ctgcttcagc cagcgggcta ccgtggcgcc aacgaccgat tcccccatct
     4921 cgggtacgat gatgttagtc ggcatggccg gcgtgcctcc cggactacgt ccattcggac
     4981 gagatgacga cgtcttccaa cacgtgcgat tcgaggttat aggcgcgctc gaccagggcc
     5041 ttctggttgg cgacatgcca cgcggaggac ccctccgcag ggctggagtt ggggggtcgg
     5101 ccgatgtagc ggagcggcca gcgcccggcg atgagcttgc cgagccgtgg ctgcatgaag
     5161 gtccaggcgc ccatgttctc cggctcctcc tggaaccaga ccacctcttc cagccgcggg
     5221 tacgcctcca gaagcgcttg caggtccccg gcggggaaga ggtacagttg ctccacacga
     5281 gcgatggcga tcaccggggt ctcctcccgc agcgggttcg tcaccaggtc cacgtagact
     5341 ttgccgctgc agaggatgag gcgccgcacc tgttcgggct ggtgccgggc ctcccgatcg
     5401 tcgatcaccg gctgccactg gccttcggcc agctcgcgca gggcggaggc cgctcgggga
     5461 tggcgtaaga ggctcttcgg ggtcatcacc agcagcggga gcgggtcggt ttccaggagg
     5521 gaggcctgcc gtcggagcag gtggaagtac tgggcggcgg tggtgcagtt ggcgatccgc
     5581 atgttggtct ccgccgccag tcccaggaag cgctcgagcc ggccgctgga gtggtccggg
     5641 ccctgcccct cgtagccgtg gggaagcagc aggaccagag agggcgtctg cccccacttg
     5701 gcgcgggcgg agacgaggaa ctcgtcgatc atcacctggg ccgcgttgat gaagtcgccg
     5761 tactgagcct cccagatcac gaggcgccgc ggctcctgga tgtcgtagcc atactcgtag
     5821 ccgacgacgg cggtctccgt cagcgggctg ttgtggatct cgaaggaggc cctggcctgg
     5881 ggaaagtttt gcaggggcgt gaacgtcttc cccgtcttca cgtcgtgaaa gacggcgtgg
     5941 cgctggccaa acgtccctcg ctccacgtcc tcgcccgtca tgcggatggc gatgccgtct
     6001 gccaggatgg aggccagggc cagctcctcc gccgtggccc attcgatgga tggctcgtcc
     6061 aggtcgtcca gagaccgcag atggcgccgt atggcgcgct ccagctttgg attgatggtg
     6121 aagtcgccgg cgagcttatg gagcgccgcg tgcatttccc gcagccgctc ggcaggcacg
     6181 gcagtctgga cccttcgggc cgccccgggg ggcggcgccg ccgggaacgg ctctgccagg
     6241 tcacgctcgg ggtgcaggga
//
LOCUS       DBNGZC010000411         2361 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_620615, whole genome shotgun sequence.
ACCESSION   DBNGZC010000411 DBNGZC010000000
VERSION     DBNGZC010000411.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_620615"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="AC16G0_07285"
     CDS             complement(<1..705)
                     /locus_tag="AC16G0_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR5099460.1"
                     /translation="MEPEIAKAVRSHVHQCAECSADLQAEVEIRSLIRAQAPRYTAPP
                     GLRTRIQANMVQDTPKVWSLWLGWLRGHAWATRSLAGAMAVLLLVWAGGLWLTRDPLS
                     ATMTRAVQEHSEYVQDMMNRPAPDPQALVRELRTQAGFPFEPVFQGDAGVQLVGTLMT
                     DLPGKRAATLVYRNSAGQYTTLFLMPGTGTVIPDGGRMQIESFKPYHRVTSGRQVLLW
                     KQKDLACLIVSDLDQSG"
     gene            complement(776..1318)
                     /locus_tag="AC16G0_07290"
     CDS             complement(776..1318)
                     /locus_tag="AC16G0_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR5099461.1"
                     /translation="MDYSHSPVAAEALNHLDALYNLARWLTRDPVEAEDLVQETYLRA
                     LKAIHQFQPGTNLRAWLFQILRNTFFTLYKRKGREPNAVDPEVMDGLSSRGLSDFGRE
                     NGGENSLAGPRDGTAGLDLTAALNRLPEEYRSVVLLADVEDFSMGEIAGIMDCPVGTV
                     KSRLFRARSILRELLRDYVR"
     gene            complement(1703..>2361)
                     /locus_tag="AC16G0_07295"
     CDS             complement(1703..>2361)
                     /locus_tag="AC16G0_07295"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5099462.1"
                     /translation="RLTQGVAYALFLTSNLALLADLAPPSRRAEAVGIFGVSGLVSIA
                     LAPAIGEVILNAWGFQVFFAATVLIALGALGVCLATPVPISESAEIPPGFGPGFWRIF
                     SPILTAAFQFGLASSIVFVFLPPFARYVGLPRVGPFYVVYTCAAIAVRFLGGQLADRL
                     GRKKIIFPSLVGLATGILLFSLLHSTWLLLMIALINGTSHGFLYPAASAFVFDRAPPG
                     "
BASE COUNT          511 a          765 c          697 g          388 t
ORIGIN      
        1 gccggactga tccaggtccg agacgatgag gcaggccaga tctttctgtt tccacaggag
       61 cacctggcgg cccgaggtaa ctcgatggta aggtttgaac gattcgatct gcatgcgacc
      121 gccgtcaggg ataaccgtgc ccgtccctgg catgaggaac agcgtggtgt actgtcccgc
      181 ggaattccga tacaccaggg tggcggcccg ttttcccggc aaatcggtca tcagggtacc
      241 caccaattgc accccggcgt cgccttggaa gaccggctca aaggggaacc ctgcctgcgt
      301 tcgaagctcc cggaccagcg cctggggatc aggtgcgggc cggttcatca tatcctgcac
      361 atattccgag tgctcttgca ctgcacgggt catcgtcgcg gagaggggat cccgcgtcag
      421 ccatagacct ccggcccaga ccaggaggag caccgccatg gccccggcta ggcttctggt
      481 cgcccaggca tgaccgcgca accacccaag ccacagcgac cagaccttcg gggtgtcttg
      541 aaccatgttc gcctgaatgc gggtgcgcaa tcccggtggg gccgtgtacc tgggcgcctg
      601 cgcccggatc aggctgcgga tctcaacctc cgcctgaaga tcggcactac actcggcaca
      661 ctgatgcacg tgcgaccgga ccgccttggc gatctcgggc tccaattccc ctcggttcaa
      721 gtcacacaag tgttctttga cttcggcaca ccgcatcggc tcaattccta gccatctagc
      781 gcacgtaatc ccgcagcaac tctcgcagaa tcgaccttgc ccggaagagt cgggatttca
      841 cagtccccac ggggcaatcc atgatcccgg cgatttcccc catgctgaag tcctccacgt
      901 cggccaggag gactacagaa cgatactcct caggcagccg gttcagggca gccgtgagat
      961 ccaagcctgc cgtcccatcc ctgggtccgg ccaggctgtt ttctccccca ttttccctgc
     1021 cgaagtcgga taagccccga gaagataagc catccatgac ttccgggtcc acggcattcg
     1081 gctctcgacc ctttcgcttg tacagggtga agaatgtgtt cctcagtatt tgaaacaacc
     1141 aggcgcgtaa attagttccc ggctggaact gatgaattgc cttgagggca cgcaggtagg
     1201 tctcctggac cagatcctcc gcctccaccg gatcccgggt gagccagcgg gccaggttat
     1261 agagggcatc cagatggttc agggcctcgg cggccaccgg gctgtgtgag tagtccatgg
     1321 ctgtgagcag gatctgttag tggggtttgg gattcgtaga ggctaccaga acaacactga
     1381 gcacgcaagc cgacgattct gctccgtatt gcccggaagc ctcccgcccg agtcctcgcc
     1441 gaggaaccag gacccacggg gtgtgcaccc gatgcccaga cgcctgccaa aaacgcgcca
     1501 gaggacaaat gaaccggcat ggaatcttcg gccacagcta ccgccgtcgc cagaacacac
     1561 ccgctccgac gaccagaatg ctccccacta agaggaaccc cgctcggaac cccaacagct
     1621 caactagcca gccaaaaccg aaggccccta ccgcaccgcc cagcagcacg gcgatattat
     1681 agatagccag cgccttgccc cgtcaaccgg gaggcgcccg atcaaacacg aaagcgctgg
     1741 ccgccggata gaggaaccca tgggatgtgc cgttgatcag agcgatcatg agaagaagcc
     1801 atgtggaatg cagcaggctg aacagcagta tgcctgtggc cagccccacc agggacggga
     1861 agatgatttt cttcctcccc aggcgatcgg ccagttgtcc ccccagaaag cggacggcga
     1921 tggccgcaca ggtatacacg acgtagaatg gtccgacccg cggaaggccg acgtaccggg
     1981 caaacggtgg aaggaataca aagacgatcg agctggcgag accgaattga aaggcggctg
     2041 tcaggatcgg tgagaagatt cgccagaaac ccgggccgaa ccccggaggg atctcggcag
     2101 actcagagat cgggaccggg gtcgccaggc agacccccaa cgcccccaga gcgatcaata
     2161 cggtcgcggc aaagaatacc tggaaccccc aggcattcag aatcacctcc ccgatggccg
     2221 gcgccagcgc tatgctgacg aggccggaga ccccgaagat cccgaccgcc tcggcgcgcc
     2281 ggctgggtgg cgcgaggtcc gccagcaggg ccaggttgct ggtgaggaag agcgcgtacg
     2341 cgaccccctg ggtcaagcgc a
//
LOCUS       DBNGZC010000412         3283 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2578350, whole genome shotgun sequence.
ACCESSION   DBNGZC010000412 DBNGZC010000000
VERSION     DBNGZC010000412.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2578350"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..467
                     /gene="erpA"
                     /locus_tag="AC16G0_07300"
     CDS             144..467
                     /gene="erpA"
                     /locus_tag="AC16G0_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006447319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HIR5099463.1"
                     /translation="MITVTSLAAEKIKNIKTSQAAPEDAGLRVRVVGGGCSGFQYQLV
                     FDAPKAGDQVYEQDGVKVVVDPKSFLYLNGTEIDYVDGLMGAGFSLKNPNARGTCGCG
                     SSFQA"
     gene            540..692
                     /locus_tag="AC16G0_07305"
     CDS             540..692
                     /locus_tag="AC16G0_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099464.1"
                     /translation="MAVTCLRSAGRGSVGGNFCTEGDAPLPLTGEEGCYSIFGWNECW
                     GWILVG"
     gene            complement(906..1028)
                     /locus_tag="AC16G0_07310"
     CDS             complement(906..1028)
                     /locus_tag="AC16G0_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099465.1"
                     /translation="MTHGIREHQVLIAQEGGTTERGLLNLRRIRSMPVPVETPI"
     gene            1118..1681
                     /locus_tag="AC16G0_07315"
     CDS             1118..1681
                     /locus_tag="AC16G0_07315"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HIR5099466.1"
                     /translation="MSSELTIVPDVTQAGSPQKEDEEFERGLLLTSLDKMVNWARRSS
                     IWPATFGLACCAIEMMATGASRFDLARFGAEVFRASPRQADLMIVAGRVSRKMAPVLR
                     KIYDQMPDPKWVIAMGACASCGGVFNTYTIVQGVDQVVPVDVYVPGCPPRPEQLIHGI
                     MLLQEKIKKARNSRKDLINFPWEQINS"
     gene            2068..>3283
                     /locus_tag="AC16G0_07320"
     CDS             2068..>3283
                     /locus_tag="AC16G0_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014378108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR5099467.1"
                     /translation="MTVETQTSTPLSPKQHEQRKSVVIRFAGDSGDGMQLTGMQFTTE
                     SALAGNDLGTLPDFPAEIRAPAGTLAGVSGFQLNFSSQEVYTPGDNPDVLVAMNPAAL
                     KVNIEDLKLGGLLLVDRDGFDAGNLKKAGYATNPLEDGSLEKFQVSVVDMTGLTLKVL
                     EDLKLSNKVATRCKNFFALGLCSWLSSRPIEPTLKWIQDKFKRTPELVEANSRVLKAG
                     YNFGETTEMFAVQYEVEPARIPPGTYRHVTGNSALAMGLVAAANKAGLPLFLGSYPIT
                     PASDILHELAQYKNFGVYTFQAEDEIAGVCSALGASFGGSIGVTTSSGPGIALKQEAI
                     NLAVMVELPLVICDVQRGGPSTGLHTKTEQADLYQAIFGRNSDSPLPVIAAATPGDCF
                     AMAYEAVRIAIKY"
BASE COUNT          643 a          980 c         1061 g          599 t
ORIGIN      
        1 cgttgcccgg tgatcacctc gcgcgcccgg atccccctgc acatgcaggc ggtgatcgag
       61 gcggcatgat gtccgaaacc gccgccctga agcagcgggc ttctggtcgg taaacgcgaa
      121 attcaacaca aggagattga cctatgatca ctgtcacctc gctcgccgcc gagaagatca
      181 agaacatcaa gacgtcccag gcggcaccgg aggatgccgg ccttcgggtt cgggtggtcg
      241 gcggcggctg ctccggcttc cagtaccagc tcgtcttcga tgcccccaag gctggcgatc
      301 aggtgtacga gcaggacggc gtgaaggtcg tcgtggaccc aaagtctttc ctgtacctga
      361 acgggaccga gatcgactac gtggacggcc tcatgggggc cggcttttcg ctgaagaacc
      421 ccaacgcccg gggaacctgc ggctgcggct ccagctttca ggcctagcgc cgaacatcgg
      481 accagactgg ccattgagag atcctgcaac cggacttggg tttgggcgag gcgcctcgta
      541 tggctgtcac ctgcctcagg tctgcaggcc ggggttcggt cgggggaaac ttctgcaccg
      601 agggcgatgc acccttgccg ctcacggggg aggagggctg ctactcgatc tttggctgga
      661 acgagtgctg gggctggatc ctggtcggct gaaggtcgga tacttcgagc tagccccaat
      721 ccgaagaaat ccgtctctcg caaagacgcc aagggcgcaa agaaaggcca gatttaatca
      781 cggaatcacc catatcggat gactacttcc aggggcggtg ggtctcttgc cttctgtgcc
      841 cagattatga tcgtgggtct tggcgaactt tgcgtcttgg cgagatgccg aatttcggat
      901 cagagctata tgggcgtctc caccgggacg ggcatgcttc ggattcgccg gaggttcagg
      961 aggccacgct ccgtcgttcc gccttcctga gcgatgagta cctggtgctc cctgatcccg
     1021 tgggtcatca cctggacgaa agcgcataca ccgaccgagc gttgcaggcg gtggaagagt
     1081 cgtggacgcc ggaggcttcc aggaaaggcg acgagtaatg agcagtgaat tgacgattgt
     1141 gccggacgtt acgcaggcgg gttcccccca aaaggaagac gaggaattcg agcgcggtct
     1201 cctcctcacg tccctggata agatggtgaa ctgggctcgt cggtcgtcta tctggccggc
     1261 gaccttcggg cttgcctgtt gtgccattga aatgatggcc acgggtgcct cgaggttcga
     1321 cctggctcgt ttcggggccg aagtgtttcg cgcttccccc cggcaggcgg atctgatgat
     1381 cgtggccggt cgagtcagtc gaaagatggc ccccgtcctc cggaagatct acgatcagat
     1441 gcccgatccc aaatgggtga tcgccatggg cgcgtgtgcc tcctgcggtg gggtgttcaa
     1501 cacctacacc atcgtccagg gagtggacca ggtggttccc gtggatgtat atgtgccggg
     1561 ctgtcccccg cggcctgagc aactcatcca tggaatcatg ctcctccagg agaagatcaa
     1621 gaaggctaga aactcccgga aggaccttat aaactttccc tgggaacaaa ttaattctta
     1681 aggatttgtt aggattcatg ccttgtcaca ctcaactcct ccgaaggctg acgacggcgg
     1741 ggctgcatgt ccaagcaagg ttgtcgccat tgatttctga accaccaagc cagcggacgg
     1801 gcgcaaacgg tggaggtcta gccaaccacc gctcttcgcg agcaggacat ccaacgcatg
     1861 gtggaggagt cagccggaac acgcccacaa cggcattgag ccgtgactcg tggagcgggc
     1921 cgctcgcgag cgattggaag atcgactcca ggactccggg gcgaggattg attttttgat
     1981 ttagtgagga tgcggcggct gggcagcggc ggtgagccac caacatcctg agtgtaaccg
     2041 cggggtgaag aggagcacga gggaatcatg acagtcgaga cgcagacgtc gacgccacta
     2101 tctcccaagc agcatgagca gcgaaagagc gtggtcatcc gcttcgcggg cgactcggga
     2161 gacggcatgc agctcaccgg catgcagttc accaccgagt ccgctctggc ggggaacgat
     2221 ctcggaaccc tgccggactt cccggcggag atccgggcgc cggcagggac gcttgccggc
     2281 gtgagcggct tccagctcaa cttcagcagc caggaggtct acacccccgg ggacaacccc
     2341 gatgtccttg tggccatgaa tccggcggcc ctgaaggtga acatcgaaga cctcaagctg
     2401 gggggtctgc tcttggtcga cagggatggg ttcgatgcgg ggaacctgaa gaaggccggg
     2461 tatgcgacca accccctgga ggacggctcg ctcgagaagt tccaggtcag cgtggtggac
     2521 atgaccgggc tgaccctgaa ggtcctggaa gacctcaagc tgtccaacaa ggtggccacc
     2581 cgctgcaaga acttctttgc cctggggctg tgctcgtggt tgtccagccg cccgatcgag
     2641 ccgaccctga agtggatcca ggacaagttc aagcggaccc ctgagctggt cgaggcgaat
     2701 tcccgcgtcc tgaaggcggg ctacaacttc ggcgagacca cggagatgtt cgccgtccag
     2761 tacgaggtcg agccggcgcg gatccccccg ggcacctacc gacacgtgac cgggaactcg
     2821 gccctggcca tgggcctggt ggctgccgcc aacaaggcgg gcctgccgct gttcctggga
     2881 agctatccca tcacgccggc cagcgacatt ctgcacgagc tggcgcagta caagaacttc
     2941 ggggtctaca ccttccaggc cgaagacgag atcgccggcg tgtgctcggc cctgggggcc
     3001 tccttcgggg ggtccatcgg tgtcacgacc agcagcgggc cggggattgc cctgaagcag
     3061 gaggccatca acctggcggt gatggtcgag ttacccctgg tgatctgcga cgtccagcgg
     3121 ggcggcccca gcacagggct ccacaccaag acagagcagg ccgacctgta ccaggccatc
     3181 ttcggccgga acagtgactc cccgctaccc gtgatcgcgg ccgcgacgcc cggcgactgc
     3241 ttcgccatgg cctacgaggc ggtgcggatt gccatcaagt aca
//
LOCUS       DBNGZC010000413         2715 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_718106, whole genome shotgun sequence.
ACCESSION   DBNGZC010000413 DBNGZC010000000
VERSION     DBNGZC010000413.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_718106"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="AC16G0_07325"
     CDS             complement(<1..676)
                     /locus_tag="AC16G0_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005369571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5099468.1"
                     /translation="MAVVTGATGAIGKAIARGIAGNPGYEVVLACRDEEKATRAVREI
                     VQGTGNAKVRYELVDVSRKASIQALADRWPGPLHVLVNNAAATPRKRQGTPEGIELQF
                     ATNVLGYFWMIQAFTDVLRQSAPARVVNVASYWAGDLDLTDLEFRRRRYNNNEVYRQS
                     KQADRMLTAAFAERLQPFGISVNACHPGDVNSALSNNLGFAGQQTSDEGARTPVWLAV
                     GPAGQQA"
     gene            complement(729..1208)
                     /locus_tag="AC16G0_07330"
     CDS             complement(729..1208)
                     /locus_tag="AC16G0_07330"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005809474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5099469.1"
                     /translation="MIRCATEQDVPLILKFIRGLAEYERLAHEVAATEELLRQTLFGD
                     RRVAEVIIGDYAGEPVAFALFFHNFSTFLGRPGIYLEDLFVKPEMRGRGFGRVLLTYL
                     AKLAKERNCGRVEWSVLDWNQPAIVFYKNLGAVPMNEWTIHRLTGEALDRQAQEFEG"
     gene            complement(1402..2415)
                     /gene="aroF"
                     /locus_tag="AC16G0_07335"
     CDS             complement(1402..2415)
                     /gene="aroF"
                     /locus_tag="AC16G0_07335"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017015881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR5099470.1"
                     /translation="MIIVLTPGASKKDADQILPQIAAVGLKPLYMPGTERIVLGAIGD
                     ERILGKLHIENHPLVEKVSPVLTPYKLVSREIHPRDTIVKIGGVPVGGKHFTVIAGPC
                     AVESPRQMLLTAQAVKRAGAKCLRGGAYKPRTSPYSFQGLGLEGLQILHKVSQRVKLP
                     AVTEVVEVADVAAVEQHASAFQVGARNMQNFRLLKALGQSRKPVILKRGMAASVDDLL
                     LAAEYILSEGNADVILCERGIRTFETATRNTLDLNAIPHIKQHSHLPVIVDPSHGTGV
                     REYVTPMAKAAVACGADGLLVEVHPHPAEALSDGRQSLDLRQFAQLMRGLRPFVEAAG
                     RRM"
BASE COUNT          441 a          917 c          837 g          520 t
ORIGIN      
        1 tcgcctgctg gccggcaggt cccaccgcca gccagacggg cgtccgggca ccctcgtctg
       61 acgtttgctg gcccgcaaag cctaaattgt tgctgagggc ggagttcacg tcgcccgggt
      121 ggcaggcgtt tacggagatg ccgaagggct ggagtcgctc cgcaaaggcc gcggtcagca
      181 tccggtcggc ttgcttggac tgccggtata cctcgttgtt gttgtacctg cggcgccgga
      241 actccagatc ggtaaggtcg agatcgcccg cccagtaact ggcgacgttc accacccgag
      301 cgggtgctga ttgcctgagc acgtcggtga atgcctggat catccagaag tagcccagta
      361 cgttggtcgc gaattgcagc tcgattccct cgggcgtccc ttgccgcttg cgcggggtcg
      421 cggcggcatt gttgaccagc acgtggagcg gccccggcca ccggtcggcc aacgcctgaa
      481 tggaggcttt tcgcgagaca tcaaccagct cgtatcggac cttggcgttg ccggtgccct
      541 gcacaatttc ccggacggcc cgcgtcgcct tctcctcgtc ccggcatgcg aggaccacct
      601 cgtaccccgg gttccccgcg atccccctgg cgatcgcctt cccgatcgcc cccgttgcgc
      661 ccgtcacaac ggccacacgg cttcccctgg tcaatattct ctcccgtcac tcgcggttgt
      721 cccgtggctc atccctcgaa ctcctgggcc tgtctgtcca gagcctcgcc ggtgagccgg
      781 tgtatggtcc attcgttcat cggaacggcg cccaggttct tgtagaacac gatagccggc
      841 tggttccagt caaggaccga ccactccaca cggccgcaat tccgctcctt ggccagcttt
      901 gccaggtagg tgagcaagac ccgtccgaat ccacgtcccc gcatctccgg cttcacgaac
      961 agatcctcga ggtagattcc aggccgcccc aggaacgtcg agaagttgtg aaagaatagg
     1021 gcaaaggcca cgggctcccc cgcgtagtcg ccgatgatca cctcggccac ccgccgatcc
     1081 ccgaagaggg tctggcggag caactcctcc gttgccgcca cttcgtgggc caatctctcg
     1141 tactccgcca atccccggat gaacttaagg atcaggggca catcctgctc ggtcgcacag
     1201 cgtatcaaga aacccggagt cctcgttgca atcggttcgg tcacgtgtat tgcctcaggc
     1261 tctgatggtt gggagtgaaa gtcgggcagt tcctgatcag actgagtaag tccgccgtat
     1321 ccgactcgaa catgccggtg tctgtctact ggttcctgga gacatgcagg ccgtgtgtcc
     1381 tgtgaaatcg gcgctcattg cctacatcct tcgccccgcg gcctccacga acggacgcag
     1441 acccctcatg agctgggcga actgcctgag gtcgagggat tggcggccgt cggagagcgc
     1501 ctccgcgggg tgggggtgga cctcgaccag gaggccgtcg gctccgcagg ccacggcggc
     1561 tttggccatg ggcgtcacgt actcgcgaac gccggttcca tgcgacggat ccacgatcac
     1621 gggcaggtgg ctgtgctgct tgatgtgggg gatggcgttc aggtcgagcg tgttacgcgt
     1681 ggccgtctcg aacgtgcgaa ttccgcgctc acacaggatc acgtcggcgt ttccctcgga
     1741 gagaatgtac tcggcggcca ggagaaggtc gtccacggac gccgccatgc cgcgcttgag
     1801 gatgacgggc ttccggctct gccccagcgc cttcagcaag cggaagttct gcatgttccg
     1861 cgcgccgacc tggaaggcgg aggcgtgctg ctcgacggcg gccacgtccg ccacctccac
     1921 gacttcggtc acggccggca gcttcacccg ctgcgagacc ttgtggagga tctgcaggcc
     1981 ttccaatccc aacccctgaa aactgtacgg gcttgtccgc ggcttgtacg ccccgccccg
     2041 caggcacttg gccccggctc gcttgaccgc ctgcgccgtc agaagcatct gacgtgggct
     2101 ctccaccgca caggggccgg caatcaccgt gaagtgcttc ccgcccacgg gaaccccgcc
     2161 gatcttgacg atggtgtctc tgggatggat ctcgcggctg accagcttgt acggcgtgag
     2221 gaccggcgac accttctcca ccagcgggtg gttctcgatg tgcagctttc ccaggatgcg
     2281 ctcgtcgccg atggcaccga ggacgatgcg ctcggtcccg ggcatgtaga gcggcttgag
     2341 ccccaccgcc gcgatctggg ggaggatctg atcggcgtcc ttcttgctgg cgcccggcgt
     2401 gagcacgatg atcatgaatg tccctctcgc gcgtctgggt tcgccgcctc gtcagtgact
     2461 cattgaccgt cacggcgcca agcacgccgg ggcggcatgg atggtcagga cccggggacg
     2521 tctctacccg aaaccgctga tggccacccc aggccttttg actccgagat gaacgctgca
     2581 caccatgacg acgcgagtat ccagcgctga ccctgggtgc tctccgcgat aggattcttt
     2641 ctgcggcttc caacttcccg gccgaaccgc ctcagcagag cgatgccgga tccacgtcca
     2701 ggtccaggcg caccc
//
LOCUS       DBNGZC010000414         4053 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2090197, whole genome shotgun sequence.
ACCESSION   DBNGZC010000414 DBNGZC010000000
VERSION     DBNGZC010000414.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2090197"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="AC16G0_07340"
     CDS             <1..526
                     /locus_tag="AC16G0_07340"
                     /inference="COORDINATES: protein motif:HMM:NF017197.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HIR5099471.1"
                     /translation="IPADGVKKVDALVAALSAKPSLKLEIACYVSPEADREGLKQYLL
                     QRKVKAQKLNDLVKKGAPAVPVDEVSLAPEEYEQYLTLAYRAEKFPKPRNFVGMVKSL
                     PVAEMEKLMLTHIEAGEEELRALAAQRANAVRDAILQSGKVGAERLFIVEPKGLAPEM
                     KDKVKDSRAEFKIA"
     gene            768..2048
                     /gene="hutI"
                     /locus_tag="AC16G0_07345"
     CDS             768..2048
                     /gene="hutI"
                     /locus_tag="AC16G0_07345"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HIR5099472.1"
                     /translation="MIQADLIIEGAAELLTLRGISVRPRRGEEMVELGIIRQGALAAR
                     RGKIVWVGLTADLLTSVKPLAFSKLISAYGKTVMPGLVDPHTHLVFAGSRENEFAMRI
                     QGKTYQEIAAAGGGINATVAATRKASKDELRANGRAALDRMLVLGTTTLEAKSGYGLD
                     LPSEIKMLEVIRELNEWGPATVIPTFMGAHEIPPEMRQNPEAFVDLVITRMIPEVAAQ
                     KLARFCDVFCESGVFSVEQSERIFRAAQAAGLESRVHADEMTDLGGAAMAARIKARTA
                     DHLLHASDDGIRQMAQAGVIAVLLPGTAYFLHLQRYARARDMIAAGVPVALATDFNPG
                     SCMTESLPLIMNLACTQMRMLPAEAITAATINAAWAIGEEDCAGSLEIGKQADVLILD
                     APGHEHLCYHFGVNLVESVIKNGKVVAERGHRVR"
     gene            2062..>4053
                     /locus_tag="AC16G0_07350"
     CDS             2062..>4053
                     /locus_tag="AC16G0_07350"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR5099473.1"
                     /translation="MDRIPNPTPTPSGGAGAMLADLLASLAAIGRSMQEEFDPRRFLS
                     QFSAHIGRLIPHDRLVIDLLHEDGRTFTVFAEHAPPELTLHQDYYTTAFAPLARYVVA
                     EWVLRTIFAGETMLVHDLQKDARFTQANPFERKLLEAGLRSALLVPLDSGGRVIGAIV
                     ATSLQPETYTQVHLERAKHVAALIGPFLENVVLLQRERRRRRRLRALEGLAHALGVSL
                     NVRDVFARLADAVRPVLDFDVMSVALLTASGREVEMLAEVDNAPGAETPPRIPLEHFS
                     ISSQVESGDVVLINDALQELTPDLPGDQLIIEGGGRSCLFAPLRFGERVGGGLYFGKR
                     QPNWFDRSDVEIATAVSAVVVLAVQHQHLAEDQRRLAVVEDRARKLEQRVDSLRHALS
                     ERYGFDQIIGRAPALRETLTRAAKVAPTETTVLITGESGTGKEVVARAIHSASPRADG
                     PFVAINCAALPDTLLESELFGHERGAFTGADRQKPGRFELAAGGTLFLDEIGELSPTV
                     QAKLLRVLQEREFQRLGGTTTLKADARLIAASNRDLAREVEAGRFREDLYYRLNVFSV
                     HLPPLRDRGEDVLLLADHFVRVLGARMAKGEPGLSRDAREALLGHTWPGNIRELQNAV
                     ERALIVSEGGLLTADQLGIAPRRERTADTVAAASPSGPTM"
BASE COUNT          685 a         1388 c         1339 g          641 t
ORIGIN      
        1 gatcccggcg gatggcgtaa agaaggttga cgccctcgtc gccgccctct ccgcaaagcc
       61 ctcgttgaag ctggagatcg cgtgctacgt gtccccggag gcggaccggg aagggttgaa
      121 gcagtacctc ttgcagcgga aggtcaaggc ccagaagctg aacgacctag tgaagaaggg
      181 cgcccctgcg gtgccggtgg acgaggtcag cctggcaccc gaggaatacg agcagtatct
      241 caccctggcg tatcgggcag agaagttccc caagccgcgg aacttcgtgg gtatggtgaa
      301 gagccttccc gttgccgaga tggagaagct catgctcacc cacatcgagg cgggtgaaga
      361 ggagctccgt gccctcgccg cacagcgggc gaacgccgtg agggacgcga tccttcaatc
      421 cggaaaggtt ggcgccgagc ggcttttcat cgtcgagccg aaaggcctcg cgcctgaaat
      481 gaaggacaag gtcaaggata gccgggccga gttcaagatc gcgtagcagg ctgctgaaaa
      541 aggcccatct gcggtgaccc acctccggtg ggtaccccgc acgctggggc actcgttgcg
      601 gcgtaccgga agtacgcctg gacccagcct cgctgggtgc cccgccccgg gtacccaacc
      661 gaaggatggg tgacgccgcc ttgcatctgg acctttttga gcagcctggg cgaaggcggc
      721 cttttcaacg tcttgctcgc caacagattc ggttcaggag acgcaccatg attcaggcgg
      781 atctgatcat cgaaggagca gccgaactcc tcaccttgcg cggcatctct gtccggccgc
      841 gccggggcga ggagatggtg gagctcggca tcatccggca gggggcgctg gccgcccggc
      901 gcggcaagat cgtctgggtg gggctcacgg cggatctgct gaccagcgtg aaacctctgg
      961 ccttctccaa gctcatcagc gcctacggca agaccgtcat gcccggtctc gtggacccgc
     1021 acacccatct ggtcttcgcc ggcagccgtg agaacgagtt cgccatgcgc attcagggca
     1081 agacctatca ggagatcgcg gcggccggcg gagggatcaa cgccacggtg gcggccaccc
     1141 gcaaggcgag caaggacgag ctgcgggcga acgggcgggc cgccctggac cgcatgctcg
     1201 tgctcggcac gaccaccttg gaggccaaga gcgggtacgg actggacctt cccagcgaga
     1261 tcaagatgct ggaggtgatc cgggagttga acgagtgggg tccggcgacc gtcattccga
     1321 ccttcatggg ggcccacgag attccgccgg agatgcgtca gaaccccgag gccttcgtgg
     1381 acctggtcat cacgcggatg atcccggagg tcgcggcgca gaagctcgcc cgcttctgcg
     1441 acgtgttctg cgagtccggc gtgttctccg tggagcaatc ggagcggatc ttccgcgcag
     1501 cccaggctgc gggcctggag tcgcgggtcc acgccgacga gatgaccgac ctgggaggtg
     1561 ccgccatggc ggcccggatc aaggcgcgga ccgccgatca tctcttgcac gccagcgacg
     1621 acggcatccg ccagatggcg caggccgggg tgatcgccgt cctcctcccc ggcacggcct
     1681 acttccttca cctgcagcgc tacgcccgcg cccgggacat gatcgccgcg ggggtccccg
     1741 tggccctggc caccgatttc aatccggggt cgtgcatgac cgagtccctg cccctcatca
     1801 tgaacctggc atgcacccag atgcgaatgc tccccgccga ggcgatcacg gcagccacca
     1861 tcaacgccgc ctgggcgatc ggtgaagagg actgcgccgg cagcctggag atcggcaagc
     1921 aggccgacgt cctcatcctg gacgcccccg gccacgaaca cctctgctac cacttcggcg
     1981 tgaacctggt cgagagcgtg atcaagaacg gcaaggtcgt cgcggagcgc ggccatcggg
     2041 tccgctgaac acaggccggg aatggatcgg atcccgaatc ccaccccgac tccgtccggc
     2101 ggtgccggcg ccatgctggc cgacctgctg gcctccctgg cggccatcgg gcggtccatg
     2161 caggaggagt tcgacccccg gcgcttcctc agccagttct ccgcgcacat cgggcggctg
     2221 atcccccacg ataggctggt gatcgacctc ctccacgaag acgggcggac cttcaccgtc
     2281 ttcgccgagc atgctccacc cgagctgact ctccaccaag attactacac caccgccttc
     2341 gctccgctgg cgcgctacgt ggtggccgaa tgggtgctcc gcaccatctt cgccggcgag
     2401 acgatgctgg tgcacgatct ccagaaagat gcccggttca cgcaggccaa tccatttgag
     2461 cgcaagctcc tggaagccgg gctgcgctcg gcccttctcg tgcccctgga cagcggcgga
     2521 cgggtgatcg gcgccatcgt cgcgaccagc ctgcagccgg agacgtacac ccaggtccat
     2581 ctggagaggg ccaagcacgt ggccgctctc atcggcccct tcctggagaa cgtggtcctc
     2641 ctccagcggg agcgacgccg gcggcggcga ctccgcgccc tggaggggct ggcgcatgcg
     2701 ctgggcgtga gcctgaacgt gcgggacgtc ttcgcccggc tggccgatgc ggttcggccc
     2761 gttctcgact tcgatgtgat gagcgtggcc ctgttgacgg ccagtggtcg cgaggtggag
     2821 atgctggccg aggtggacaa cgcccccggt gccgagacgc cgccacggat cccgctggaa
     2881 cacttctcta tctcttccca agtcgaatcc ggcgatgtcg tcttgatcaa cgacgccctg
     2941 caggagttga cgccggacct ccccggggat caactgatca tcgaaggcgg cggccgatcc
     3001 tgcctcttcg ccccgctccg cttcggcgag cgtgtgggcg gcggcctcta cttcgggaag
     3061 cgtcagccca actggtttga ccggtcggac gtggagatcg ccaccgccgt ctccgcggtc
     3121 gtggtcctgg ccgtccagca ccagcacctg gccgaggacc agcggcggct ggccgtcgtc
     3181 gaggaccgcg cccggaaact cgagcagcgc gtggactcgc tccgccatgc cctctccgag
     3241 cggtacggct tcgaccagat catcgggcgg gcccctgccc ttcgcgagac cttgacccgc
     3301 gcagccaagg tggcccccac ggagaccacg gtcctgatca ctggtgagag cggaaccggg
     3361 aaagaagtgg tcgcccgcgc cattcattcc gccagccctc gggctgacgg ccccttcgtg
     3421 gcgatcaact gcgcggccct gcccgacacg ttgctggaat ccgagctgtt cggccacgaa
     3481 cggggagcct tcaccggcgc cgatcggcag aaaccgggtc gtttcgagct ggccgcaggc
     3541 ggcacgttgt tcttggacga gatcggagaa ctctcaccca ccgtccaggc caagctcctc
     3601 cgcgtcctgc aggagcggga attccagcgc ctgggcggca cgaccacgct caaggccgac
     3661 gcccggctca tcgcggccag caatcgggat ctggcccgcg aggtcgaagc cggccgcttt
     3721 cgcgaggacc tctactatcg actgaacgtc ttcagcgtgc acttgccccc gctgcgcgac
     3781 cggggggagg atgtgcttct cctggccgac cacttcgtcc gcgtgctggg ggcgcgcatg
     3841 gccaaggggg agcccggact cagccgcgat gcccgggagg cgctcttggg ccatacctgg
     3901 cccggaaaca ttcgcgagct gcagaacgcg gtcgaacgcg ccctcatcgt ctcggagggc
     3961 ggcctcctca ccgccgacca gctcgggatc gctcccagac gagagcggac cgcggacacg
     4021 gtcgcggcag cgtcgccgtc tggccccaca atg
//
LOCUS       DBNGZC010000415         1520 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1405097, whole genome shotgun sequence.
ACCESSION   DBNGZC010000415 DBNGZC010000000
VERSION     DBNGZC010000415.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1405097"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="AC16G0_07355"
     CDS             complement(<1..577)
                     /locus_tag="AC16G0_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099474.1"
                     /translation="MAALLEVEGLSKWFGGLQAVSDVSFAVPEGGIVGLIGPNGAGKS
                     TLFDLLSGVQRNTGGRVRFRGHDVTGWSAHRIAHLGMSRTFQKVRIFPGMTVLDNAMV
                     GAFLHTADPSEAQGRAEEALKMVDLSEEMRHRSTRSLTLVDRKRLEMARALCTHPSLL
                     LLDEVLAGLNQQEISVALDLIRRLNKRGVTLI"
     gene            complement(696..>1520)
                     /locus_tag="AC16G0_07360"
     CDS             complement(696..>1520)
                     /locus_tag="AC16G0_07360"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5099475.1"
                     /translation="GHGAYFGLGAYTSSLLLVRLGVTPWIGMVPGAIVAALFGLCLGY
                     PSFRLKGVYFKLVTFVAALILEIICRSWSDFTGGDPGLQIPLLRNAPAMYQFGSPVPY
                     FYIILGMASLYFLLTRRLLRSRFGYYLQALRDDQVAAEVLGVNSLYMKLAGFALSAML
                     TALVGTFYAQYVLFIDPASTFGMFVSVKIALAAIVGGAGTLWGPVIGGLLLVPVAELG
                     NAQFTGTLTGVDVVLYSLVLIAVAIFLPRGVVSLPEFIRRKRHGMQRTKAPPQLAG"
BASE COUNT          331 a          500 c          448 g          241 t
ORIGIN      
        1 cgatgagggt cactccccgt ttattgaggc gacgaatcag atccagtgcc acgctgatct
       61 cctgctgatt gagcccagcc agcacctcat cgaggagcaa gagggacgga tgggtgcaaa
      121 gcgcccgcgc catttccagc cgcttccgat ctaccagcgt gaggctccgc gtcgagcggt
      181 gtctcatttc ctcggagagg tcgaccatct tgagggcctc ttcagctcgc ccttgcgcct
      241 cggatgggtc ggccgtgtgc agaaaagcgc ccaccatggc gttatccagc acggtcatgc
      301 cgggaaagat tcggaccttc tgaaacgtgc gggacatgcc caggtgggca atccgatggg
      361 cgctccagcc ggtcacgtcg tgaccgcgga atcgcactcg tccgccggtg ttcctttgca
      421 cgccgctcag cagatcgaag agggtactct tccccgcccc gttcggcccg atcagcccta
      481 caatgccccc ttccggaacg gcaaaggaca catcgctcac cgcttgcagg ccaccaaacc
      541 atttgctcag gccctcgacc tccagcaggg cggccatagc ttctcctcac attcactgca
      601 gcaatagaat gccacgtcga ttggcggccc cgacttcagc ctggaacgct tccgcccgca
      661 ccgcatgatc ccggctagac aggggcgatc ggggatcaac ccgcaagctg gggcggtgcc
      721 ttcgtccgct gcattccgtg ccttttcctg cgaatgaact ccggcaggct caccacgccc
      781 cggggcagga agatggcgac ggcaatcaac accaggctat agagcacgac atcgacgcca
      841 gtcagggttc cggtgaactg cgcattgccc aactcggcca cagggaccag caggagccca
      901 ccgatcactg gaccccacaa ggtgcccgcg ccaccgacga tggccgccaa ggcgattttc
      961 acggagacga acatcccgaa ggtgctcgcc gggtcgatga acagcacgta ctgggcgtag
     1021 aaggtaccca ccagcgcggt cagcatggca ctgagggcga atcccgcgag tttcatatac
     1081 agagagttga cccccaacac ctccgccgcg acctggtcat cccgcaacgc ctgcaggtaa
     1141 tacccgaagc ggctgcgcag gagacgccgc gtgaggagaa agtagaggct cgccatcccg
     1201 aggatgatgt agaagtaggg aacaggcgag ccgaactggt acatcgccgg cgcgttacgc
     1261 aagaggggga tctgcaagcc tgggtcgcca ccggtgaagt ccgaccatga ccgacagatg
     1321 atctcgagga tcagggcggc cacgaaggtc accagcttga aatagacccc tttcaagcgg
     1381 aaggatggat aacccagaca cagcccaaat agggcggcga caatcgcccc gggcaccatt
     1441 ccgatccagg gcgtaacgcc cagacggacc agcagcaggg aactggtata ggcacctagc
     1501 ccgaagtagg ccccgtgtcc
//
LOCUS       DBNGZC010000416         1315 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_427060, whole genome shotgun sequence.
ACCESSION   DBNGZC010000416 DBNGZC010000000
VERSION     DBNGZC010000416.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_427060"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1315
                     /locus_tag="AC16G0_07365"
     CDS             <1..>1315
                     /locus_tag="AC16G0_07365"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR5099476.1"
                     /translation="GLDSVFAFLYIFVIFEAANLLERRGSLLVALLSAGLYGLLVAAE
                     WTRIISPVQFAGGFAPLRPVGYAVYQVLMHAVAFVAVAILSSHLAYRLRQTGRELERR
                     GLDLRNLRTLHQVIVANISSGLMTLDLTGRIISYNQAAQRITGYDFDSLRDRPWEETP
                     FAVCPSLAEFFAHPHAPLQVSLTEMSLRRRDGQLIPVGIACSTLRGSDGEPMGLVAIF
                     QDLTDRKRVEEQLRRADRLAALGQLAANIAHEIRNPLAAISGSVEILREDLALTGSNR
                     ELLDIILREAHRLKLVTGQFLDFAKPRQLLFRSCPLRPLLDETLQLLAKSGERHPETT
                     WTVREECPDLSVLADADQLRQVVWNLCLNAIQSMPTGGILTITLRPALSDGQLPLPSI
                     SPLGRTGNPKPTGESTARTPAQSASPLGEWVEMVFQDTGQGIPPQE"
BASE COUNT          213 a          457 c          409 g          236 t
ORIGIN      
        1 ggggttggac tccgtcttcg ccttcctgta catcttcgtc atcttcgagg ccgccaatct
       61 cctggagcga cgggggagcc tcctcgtggc ccttctcagc gcgggtctct acggcctgct
      121 ggtggcggcg gagtggacac ggatcatttc acccgtccaa ttcgccggcg gctttgcccc
      181 cttgcggccc gtgggctatg cggtatacca ggtcctgatg cacgccgtgg ccttcgtggc
      241 cgtggccatc ctgagcagcc acctggcgta ccgcctgcgg cagaccggga gggagttgga
      301 gcgccggggt cttgatctcc ggaatctccg gacgctacac caggttatcg tggccaacat
      361 cagcagcggc ctcatgacct tggacctcac agggcggatc atctcttaca accaggcggc
      421 ccagcggatc actgggtatg acttcgacag ccttcgggac cggccctggg aggagacccc
      481 ctttgccgtc tgccccagcc tggccgaatt cttcgcccac ccccacgccc ccctccaggt
      541 ctcactcaca gagatgagcc tccgccgccg cgacgggcag ctcattcccg taggcatcgc
      601 ctgctccacc ctgcgcggca gcgacgggga gccgatgggg ctcgtggcaa tcttccagga
      661 cctgactgat cggaagcggg tggaggagca gctgcgccgc gcggaccgat tagcggccct
      721 ggggcaactg gcggcgaaca tcgcccacga gatccggaat cccctggcgg ccatcagcgg
      781 atcggtggag atcctccgcg aggacttggc cctcacgggc tcgaaccgag agctactgga
      841 catcatcctg cgggaggcgc atcgcctgaa gctggtcacg gggcagttcc tcgactttgc
      901 caaaccccgg cagctgctct tccggtcgtg cccgctccgg ccgctcctgg atgagacgct
      961 tcagctcctg gcgaagagcg gcgagcgcca ccccgagacc acctggaccg ttcgggagga
     1021 gtgtccggac ctttccgtcc tggcggatgc ggaccaactg cgccaggtgg tgtggaacct
     1081 ttgtctgaac gccatccaat ccatgccgac gggcggcatc ctcaccatca cccttcggcc
     1141 tgcgttgagt gacggccaac tgccccttcc gtcgatcagc ccgttgggac gaaccggcaa
     1201 tccgaaaccg actggcgaat cgaccgcccg aacccctgcc cagtcggcgt cgccgcttgg
     1261 cgagtgggtg gagatggtct ttcaggatac cggacaaggc attcctccac aggaa
//
LOCUS       DBNGZC010000417         2669 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1503276, whole genome shotgun sequence.
ACCESSION   DBNGZC010000417 DBNGZC010000000
VERSION     DBNGZC010000417.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1503276"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="AC16G0_07370"
     CDS             <1..344
                     /locus_tag="AC16G0_07370"
                     /inference="COORDINATES: protein motif:HMM:NF015038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HIR5099477.1"
                     /translation="CKEMSAEERRAWRQRDAVPTLEVFGEWLDAQAGHVLPKSPIGQA
                     IAYARAQWGDLQIYTCDGALSIDNNLAERTLRAQAIGRKNFLLSSAGRVKTLAFRRRL
                     QHSSVLASSFA"
     gene            666..2090
                     /locus_tag="AC16G0_07375"
     CDS             666..2090
                     /locus_tag="AC16G0_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HIR5099478.1"
                     /translation="MTTECNAAYLDFPMLGSRQVLADFDGGDITSDGGALLLRKTEEL
                     TGIIRQFARCFTDHRNSELIEHSVEELLGQRVYGLALGYEDLNDHDDLRHDPLLATVV
                     GKADPTGDSRRRRRDRGKPLAGKSTLNRLELTPVGADAGSRSKKVTCRTRDVEHLFVS
                     LFLQAHDRPPEHIVLDLDATDDPIHGHQLGRFFHGYYKNYCYLPLYIFCGDHLLCARL
                     RPSDIDASAGALKQLQRVVAEIREAWPEVTITIRADSGFCREPIMAWCEANGVDYLLG
                     LAQNPRLVAMIATEQGQACQEFEATREPARVFAELRYRTLDSWSCERRVVAKAEHLTT
                     GANPRFVVTSLAVEDRAAKALYEENYCGRGEAENRIKEQQLHLFADRTSAGTMRANQI
                     RLLLPSIAYVLMNALRRLGLADTDLAEAQCQTSRLKLLKIGALVRVTFRKVWVSLASS
                     CPYAEVFRRVHANLARLSPLILRC"
BASE COUNT          481 a          829 c          883 g          476 t
ORIGIN      
        1 cctgcaagga gatgtcggcc gaggagcgcc gtgcctggcg gcaacgggat gcggtaccga
       61 ccctggaggt gttcggggag tggctggacg cgcaggccgg tcatgtgctg ccgaagagcc
      121 cgatcggcca ggcgatcgcc tacgcccggg cccagtgggg agacttgcag atctacacct
      181 gcgacggcgc gctgagcatc gacaacaatc tcgccgagcg gacgctgcgt gctcaggcca
      241 tcggtcggaa gaacttctta ttgtcatctg ctgggagggt caaaaccctc gcctttaggc
      301 gacggcttca gcattcttcg gtcctcgcgt caagctttgc atgagttgaa gttccggtgt
      361 cggtcgtctt tcgcaacgag accagtggtc tgcggcccct gggatttgac ggccgaggtg
      421 gtgtcgtcag ggcgcatctc ggtgggaccg agacaggcgg ataaccgcga ggagcctgga
      481 gcccgtcagc ccgcctctgg cgggaagctt tcagccgagc ttggctcgga acaggacttg
      541 agtccgttac accgaagccc ccgcctttag cccgcatttc tcaccagggg aatggaatcg
      601 ggctgcccgg cgtgtcatag gtggaattgc gacaaccatc tacgacaccg acccaaggca
      661 gcccgatgac cacagagtgt aacgcggcct acctcgactt tcccatgctc gggtcccgcc
      721 aggtcctggc cgacttcgac ggcggtgaca tcacctccga cggcggcgcc ctgctgctcc
      781 gcaagaccga ggagctgacc gggatcatcc gccagttcgc caggtgcttc accgatcacc
      841 gcaactccga gcttatcgag cactccgtcg aggaactcct cggccagcgc gtctacggcc
      901 tggcgctggg ctacgaggac ctcaacgacc acgacgacct gcggcatgat ccgctgctgg
      961 ccaccgtcgt cggcaaggcc gaccccaccg gcgactcacg acggaggcgc cgcgatcgcg
     1021 gcaagccgct ggccggcaag agcacgctga accgactcga gctgaccccc gtcggtgcgg
     1081 acgccggcag ccgctccaag aaggtcacct gccgaacccg cgacgtggaa cacctcttcg
     1141 tcagcctgtt ccttcaggcg catgaccggc cgcccgagca catcgtgctc gacctcgacg
     1201 ccaccgacga cccgatccac ggccaccagc tcggccgctt cttccacggc tattacaaga
     1261 actactgcta tctcccgttg tacatcttct gtggcgacca cctgctctgc gcgcggctgc
     1321 ggccgtcgga catcgacgcc agcgccgggg cgttgaagca actccagcgg gtcgtggcgg
     1381 agatccggga ggcctggccc gaggtcacga tcacgatccg ggccgacagc gggttctgcc
     1441 gcgagccgat catggcttgg tgtgaagcca acggcgtgga ctacctgctc ggcctggcgc
     1501 agaaccctcg gctggtggcg atgatcgcca cggagcaggg gcaggcgtgc caggagttcg
     1561 aggcgacgcg ggagcccgcg cgggtgttcg ccgagttacg gtaccggacg ctcgacagct
     1621 ggagctgcga gcggcgggtg gtggccaagg ccgagcacct gaccacgggg gccaacccgc
     1681 ggttcgtggt cacgtcactg gcggtcgagg atcgcgcggc gaaggcttta tatgaggaga
     1741 actactgcgg ccgcggcgag gcggagaacc ggatcaagga gcagcaattg cacctgttcg
     1801 ctgaccgcac cagcgccggc acgatgcggg ccaaccagat ccggctgctc ctcccatcga
     1861 tcgcttacgt gctgatgaat gcgttgcgtc ggctggggct tgccgatacg gacttggccg
     1921 aggcgcagtg ccagacgagc cggttgaagc tgttgaagat cggcgccttg gtgcgggtga
     1981 cgttccggaa ggtgtgggtg agcctggcga gcagttgtcc gtacgccgag gtcttccggc
     2041 gggtgcatgc gaacctggcc cggttgtcgc cgctgatcct gcggtgctga cctgaggaga
     2101 tgtccacgtg ccggcgggcc agaacggcct ggaggacgag gccctacgat cgtggtgcgt
     2161 cccgggtcgg ccccgaagcc ctcatcccga gggcttccga gggctcggag aggctgggaa
     2221 ggcagtgaaa ggcgacgacg cgggtatcga ggcccgctca ggccgccatt cgcagaggga
     2281 gggcggtcaa tcaggcgagc aatcggtctc cgaaagaata gggtgagaaa tgcgggctag
     2341 gcctgaaaca agcgtattgg gacggtgccc tggaacatca cccctgtaat ttcaggcttt
     2401 cgggcaaggg gtttccgagg gggctgtcaa tttcaagtga gcgccactcc gaatttcatc
     2461 gtttcggctt ttgccttgtt tcataggttt tgagccgctg acagccttat gtcaatcctc
     2521 gtattacgct tgtttaggga ctagcctgga ttcccacgga ggggaaactg attaacaatc
     2581 actggttcga tcttcgagaa aaacccgatg atctccgcct cttctccatt gggatggtta
     2641 aggcagccct ctaaacattg tcgtatgga
//
LOCUS       DBNGZC010000418         2728 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_916718, whole genome shotgun sequence.
ACCESSION   DBNGZC010000418 DBNGZC010000000
VERSION     DBNGZC010000418.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_916718"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /gene="modA"
                     /locus_tag="AC16G0_07380"
     CDS             <1..518
                     /gene="modA"
                     /locus_tag="AC16G0_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5099479.1"
                     /translation="ASYVDGLRAGGAVLADSVEPYAQGRIVLATHRGQPSLTRLTDLA
                     RDEVKRVAIANPAHAPYGMAAREALQSSGVWTKVQPKLVYGENIRQALQFLETRNVDA
                     AIIALSVAQVPDVQYTVIAASLYRPIVQVAAVTTRSQHPDLARAFIRFVNGPTGRPVM
                     KRLGFLLPGEF"
     gene            526..1176
                     /gene="modB"
                     /locus_tag="AC16G0_07385"
     CDS             526..1176
                     /gene="modB"
                     /locus_tag="AC16G0_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HIR5099480.1"
                     /translation="MDSFPILLSLRVATLSTLLAGVAGVGLAWLLTRKRLPGRPLLES
                     VVLLPLVLPPTVVGFYLLLLLGGAGPLGAALEAAGLQFIFTWRAAVLAATVASLPLVV
                     KVVQAAFEAIDPRLEAAAKTLRPAPSVFLSLTLPLAWRGILAGLVLAFARGMGEFGIT
                     LMLAGSIPGRTQTLPLAIYDAVQANDLAQANALALTTVGIVTVLLLVLSRLPRVRL"
     gene            1176..2267
                     /locus_tag="AC16G0_07390"
     CDS             1176..2267
                     /locus_tag="AC16G0_07390"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099481.1"
                     /translation="MLEFAFERRLDGFVLAPALRAEQGTTALYGPSGAGKSLTLLCLV
                     GLLHPDRGWIRLNGRTLFDAALGVHVPPHRRRVGVVFQEYALFPHLSVAANLAYGLRG
                     IGRAEIRERVLGMLRLLRLEGFGRRYPGDLSGGQRQRVALGRGLILQPELLLLDEPFS
                     ALDQMERERLRAEVLDLLQGFGGMTILVTHSLEEAYLMSRQIAVIHGGRILQAGPRDD
                     VLQHPRDRRVAEQVGASNIFQGVVTGVGTSAAVAWRGLQLTVAGPLPPPGTAVEFCIR
                     PENVRLIWPDRVAGRPNVLSGQLSAEVKRGVDYLLRFRADGSAAQASDIQIHCSEHLH
                     YLMKLEPGTPVWIALPPEKLHVLEPVAPG"
     gene            2405..>2728
                     /locus_tag="AC16G0_07395"
     CDS             2405..>2728
                     /locus_tag="AC16G0_07395"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR5099482.1"
                     /translation="MRVANVIGPREIRLDEAPIPTPGRGEVLVRIRAVGLCGSDLQYY
                     AQGRIGDQSLSSGHILGHEVAGVVEVLGPEAGGPPSGTPVVVDPAMPCGRCRFCLGGD
                     PNLCRS"
BASE COUNT          368 a          970 c          927 g          463 t
ORIGIN      
        1 atgcctccta cgtggatggc cttcgcgccg gcggggccgt cctcgccgac agcgtggagc
       61 cgtacgccca gggccgcatt gtcctggcca cgcatcgggg ccagccgtcg ctgacgaggc
      121 tcacagatct cgcccgcgac gaggtgaagc gggtggccat cgccaacccg gcgcatgcgc
      181 cttacggtat ggcggccaga gaggccctgc aatcatccgg ggtatggacg aaggtccagc
      241 cgaagctggt gtacggcgaa aacatccggc aggcgctcca gttcctggag acccgaaatg
      301 tggatgcggc gatcatcgcc ctgtcggtgg cgcaagtccc cgacgtgcaa tacaccgtca
      361 tcgccgcgag cctctaccga cccatcgtcc aggtggcggc cgtgacgaca cgctctcagc
      421 acccggacct ggcgcgggcc ttcatccgtt tcgtgaacgg gccgacgggt cgccctgtca
      481 tgaagcggct cgggtttctc ttgccggggg agttttgagc ggccaatgga ttcatttccg
      541 atcctgctgt ctctccgggt cgccacgctg agcacgctcc tggcgggcgt agcgggtgtg
      601 gggctcgcct ggctgctcac acgaaagcga ctgccgggcc gcccgctcct cgaatccgtc
      661 gtcctcctcc cgctggtcct gccccccacc gtggtgggct tttatctgtt gctcctcctg
      721 gggggggcgg ggccgctggg tgcggccctg gaggccgcgg ggctccagtt catcttcacc
      781 tggcgagccg ccgtgctcgc cgccaccgtg gcctcgctcc ccctggtggt gaaggtcgtc
      841 caggccgcct tcgaggccat cgacccgcgg cttgaggcgg cagcgaagac gctccgtccg
      901 gccccgagcg tctttctcag cctcacgctg cccctggcct ggcgcgggat cctggccggc
      961 ctcgtcctgg ccttcgcccg cgggatgggc gagttcggca tcacgctcat gctggccgga
     1021 agcatcccgg gccggacgca gaccctcccc ctggcgatct acgacgccgt gcaggccaac
     1081 gacctggccc aggcaaacgc ccttgcgctc accaccgtcg gcatcgtcac ggtcctcctc
     1141 ctcgtcctca gccggctgcc ccgggtacgc ctctgatgct ggaattcgcc ttcgagcgcc
     1201 ggctggatgg attcgtcctg gcccccgcac tccgggcgga gcaggggacc accgcgctct
     1261 acggtccgtc cggcgccggg aaaagcctca ccctcctctg cctggtgggc cttctgcacc
     1321 cggatcgcgg ctggatccgg ctcaacggtc gcacgctgtt cgacgccgct ctgggcgttc
     1381 acgtcccgcc gcaccggcgc cgcgtggggg tggtcttcca ggagtacgcc ctgtttcccc
     1441 acctcagcgt ggcggcgaat ctggcctacg gccttcgggg cattggccga gccgagatca
     1501 gggagcgggt cctgggaatg ctccgcctcc tgagactcga gggattcggc aggcgctatc
     1561 cgggggacct gtccggcggc cagcgacagc gcgtggccct gggccggggg ctcatcctgc
     1621 aaccggagct cctgctcctg gacgagcctt tctccgccct ggatcagatg gagcgcgagc
     1681 gtctgcgcgc cgaggtcctt gacctcctcc agggattcgg agggatgacc atccttgtga
     1741 cgcatagcct ggaggaggct tacctgatgt cccgccagat cgccgtcatc catggcgggc
     1801 gcatcctcca agccggcccg cgcgacgacg tgctgcagca cccccgggat cggcgcgtgg
     1861 ctgagcaggt gggggcatcc aacatcttcc agggcgtcgt gacgggggtc ggcacctctg
     1921 cggccgtcgc ctggcgtgga ctccagctca ccgtcgccgg cccgctgccg ccgccgggca
     1981 ctgccgtgga gttctgcatc cgcccggaga acgtgagact catctggccc gacagggtgg
     2041 cgggccggcc caacgtcctg tccgggcagc tcagcgccga ggtgaaacgg ggtgtggact
     2101 accttctgcg gttccgggca gacggatcgg ccgctcaggc gagcgacatc cagatccact
     2161 gctccgagca cctgcactac ctgatgaaac tcgagccggg cacaccggtc tggatcgccc
     2221 tccccccgga gaagctgcac gtgctcgaac ccgtggcgcc gggatgaggc ggcgggacgg
     2281 catccgtcca tggcggttgc aggcgccgct ccatcccttg acccctcgat agcgggcagg
     2341 catcatgcct tcgggtggcc ggaggatggt ccggccagaa aggggaggaa tcacgagcgc
     2401 gaccatgcga gtagcaaacg tcatcggccc gcgcgagatc cgattggacg aggcccctat
     2461 ccccacaccc ggtcgcggtg aggtgttggt ccgcatccgg gcggtggggc tctgcggctc
     2521 cgacctccag tattacgcgc agggacgcat tggcgaccag tcgctctcct ccggtcacat
     2581 cctgggccac gaggtggcgg gcgtcgtcga ggtcttgggt ccggaggcag gcgggccgcc
     2641 ctcgggcacg cccgtggtcg ttgatcccgc catgccgtgc ggccggtgtc ggttctgcct
     2701 gggcggggac cccaacctgt gccggagc
//
LOCUS       DBNGZC010000419         4610 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_36719, whole genome shotgun sequence.
ACCESSION   DBNGZC010000419 DBNGZC010000000
VERSION     DBNGZC010000419.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_36719"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..106)
                     /locus_tag="AC16G0_07400"
                     /pseudo
     CDS             complement(1..106)
                     /locus_tag="AC16G0_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878606.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
     gene            complement(261..608)
                     /gene="rplS"
                     /locus_tag="AC16G0_07405"
     CDS             complement(261..608)
                     /gene="rplS"
                     /locus_tag="AC16G0_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR5099483.1"
                     /translation="MSLIQAVEEKHLRKDLPSFAPGDTVKVQVKVVEGDKERLQAFEG
                     ICIRRRGGGLSATFTVRKVTYGVGVERTFPAHSPSIDRIEVLRHGQVRRAKLYYLRNL
                     KGKAARIKEYRTS"
     gene            complement(710..1426)
                     /gene="trmD"
                     /locus_tag="AC16G0_07410"
     CDS             complement(710..1426)
                     /gene="trmD"
                     /locus_tag="AC16G0_07410"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020278180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR5099484.1"
                     /translation="MFQGPLQESILRRAQDKQLVEIVVHDLRSWTTDRHHVVDDTPYG
                     GGAGMVLKPEPVFDAVGALSSEPETTVIVLSTQGVLFTQAMAGELAQAGHLILLCGRY
                     EGFDDRIPQGLRARELSIGDYVLTGGEVPALVVLDAVVRLMPGVLGDETSAQTDSFAT
                     GLLEHPQYTRPPVFRGMAVPPVLLSGDHQAIARWRRKQAILRTRDRRPDLLEKATLSE
                     VDRELLAELDEEEETGSTGD"
     gene            complement(1449..1997)
                     /gene="rimM"
                     /locus_tag="AC16G0_07415"
     CDS             complement(1449..1997)
                     /gene="rimM"
                     /locus_tag="AC16G0_07415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR5099485.1"
                     /translation="MPRPAYLILGRILKVHGVNGEVRAASFAEAWLPFQVLTHLWVGP
                     PEASPRRLRLEAGRLHGREAILKLEGVDTPAAASRLIGQQVSIPRADAPAPPEGVFYH
                     YDILGLLVVEGERPLGCVRQILETPAHDVYVIQGPAGEWLLPATRAHIRRIDLAAERI
                     EIEPMTGLVGPSSGGEGNPEAV"
     gene            complement(2001..2234)
                     /locus_tag="AC16G0_07420"
     CDS             complement(2001..2234)
                     /locus_tag="AC16G0_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIR5099486.1"
                     /translation="MYKDLVEYVAKALVDHPDQVSVESVEEPTTTTVRLKVAPADLGR
                     VIGKQGRTARAMRTLLHATAARSKRRAVLEILE"
     gene            complement(2322..2606)
                     /gene="rpsP"
                     /locus_tag="AC16G0_07425"
     CDS             complement(2322..2606)
                     /gene="rpsP"
                     /locus_tag="AC16G0_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR5099487.1"
                     /translation="MAVKIRLRRMGTKQTPFYRVVVADERTARNGRFLESLGTYDPRQ
                     TSVGLKVDADKALRWLERGAQPTDTVRTLLRTAGVLRRFAELRNKPPATP"
     gene            complement(2671..4011)
                     /gene="ffh"
                     /locus_tag="AC16G0_07430"
     CDS             complement(2671..4011)
                     /gene="ffh"
                     /locus_tag="AC16G0_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR5099488.1"
                     /translation="MFQGLSDRLNDIFKRLRGHGSLTEDNIAESLREVRMALLEADVN
                     VKVVRTFLERVRERAVGRDVLESLSPGQQVVKVVYDELTALMGGTASRLERAGQPPSV
                     VMLVGLQGSGKTTTAGKLARLLRQEGQRPLLVAADLQRPAAQEQLATLGETLGVEVYR
                     NPSAGSAVAVCREAVSLARSRLLDPVILDTAGRLHVDEPLMEELRAIKADVKPTEILM
                     VADAMTGQDAVNSATAFNHALALSGYILTKLDGDARGGAALSIRSVTGAPIKFIGVGE
                     KLDALEVFHPDRMASRILGMGDVLSLVERAEQVVDAKQAAVLQKKLKAQAFTLEDFRD
                     QLRQIRGMGPLDQLLGMIPGLSRLKGLPDSTTQERELKRVEAIIDSMTPGERQKPEIL
                     NGSRRKRIAAGSGTNVADVNRLLKQFADMQKMMRQLMQAGKGGRLPRQMPFSTR"
BASE COUNT          777 a         1588 c         1457 g          788 t
ORIGIN      
        1 tgtcggcaag ccccggcagg atcgcatcgg ccggcagcac gaccgccgcc gcgaccaccg
       61 gaccggcaag gctcccccgg cccgcctcgt ccacgcctgc cacgcggctg ccgcagggaa
      121 catccaagcc cttgcccgtc agtccgtgtc gacaggcaaa gggtagaatc gccctggagg
      181 cccccgatcc tcggtccccc gatcgtcttc gggcagaacc tgccgtcacc gccgtacgtc
      241 cttgcctctc ggcaggcgag ctacgacgtc cggtactctt tgatccgcgc cgccttgccc
      301 ttcagatttc ggagatagta cagctttgcc cggcgcacct ggccgtgtcg caggacctcg
      361 atccggtcga tgctgggcga gtgcgcggga aaggtccgct cgaccccgac gccgtacgtc
      421 accttccgga cggtgaaggt ggcgctcaga ccgccgcccc ggcgccggat gcagatccct
      481 tcgaaggcct gcaatcgctc tttgtctcct tcaaccacct tcacctgcac cttgaccgtg
      541 tcgccgggag cgaacgacgg caggtctttt cgcaagtgct tttcctctac tgcctggatc
      601 agtgacatgt gcctaccccc caatgccaac cttcaaattt caagccgagg acggttcgaa
      661 ccggccaatg tcggcttgcc ccagattccc ggcggtcagg tggccccggt caatccccgg
      721 tggagccggt ctcctcttcc tcgtccagct cggccagcag ttcccgatcc acctcggaca
      781 aggttgcctt ctctaggaga tccggccggc gatctctggt ccgcagtatc gcctgtttcc
      841 ttcgccagcg ggcaatcgcc tgatggtcac ccgagagcag gacgggcggt acggccattc
      901 cccggaaaac cggaggccgg gtgtactgag ggtgctccag gaggccggtg gcaaaggaat
      961 ccgtttgggc cgaggtctcg tcccccagga cgcccggcat cagccggacc acggcatcca
     1021 gaaccaccag cgcgggcacc tctcccccgg tgagaacata gtcgccgatg gatagctcgc
     1081 gcgcccgcag cccctgaggg atccggtcgt cgaagccctc gtagcggccg cagagcagga
     1141 tcaggtgtcc cgcctgtgcc aattcgccgg ccatcgcctg ggtgaacaag accccctggg
     1201 tggacaggac gatgaccgtg gtctcgggct cggaggagag cgcccccacg gcatcgaaga
     1261 ccggctcggg cttcagcacc atgccggccc cgccgccgta cggggtgtcg tccaccacgt
     1321 ggtgtcgatc tgtcgtccag gaccggaggt cgtggaccac gatctccacg agctgcttgt
     1381 cctgagcccg gcgcaagata ctctcttgaa ggggtccctg gaacatgccg ggaaacagcg
     1441 tcaagatatc aaaccgcttc agggttcccc tcgcctcccg acgacgggcc caccaggccg
     1501 gtcatgggct cgatctcgat gcgctcggcg gccagatcaa tccgccggat gtgggcacgg
     1561 gtggccggca gcagccactc gccggccggg ccctggatca cgtagacatc gtgggccggc
     1621 gtctccagga tctgacggac gcagccgagg ggccgctcgc cttccaccac gaggagcccc
     1681 aggatgtcat agtggtaaaa gaccccctcg ggcggggcgg gggcatcggc ccgcggaatg
     1741 gacacctgct gcccgatcag tctggaggcc gctgcgggtg tatccacccc ctccagcttc
     1801 aggatcgcct ctcgcccgtg caggcgtccg gcctccagcc tcaaacgccg gggggaggcc
     1861 tcaggtggtc ccacccagag gtgggtcaga acctggaatg gcagccacgc ctcggcgaag
     1921 ctggccgccc tcacctcgcc gttcacgccg tggaccttca ggatgcggcc caggatcagg
     1981 taggccgggc gtggcatgca tcactccaga atctccagca ccgcccgccg cttggatcgg
     2041 gccgccgtgg cgtgcagcag ggtccgcatg gcccgggccg tgcgtccctg cttgccgatg
     2101 acccggccaa ggtccgccgg cgccaccttc agccgcaccg tggtggtcgt cggctcctcc
     2161 actgactcca cactcacctg atccggatgg tccaccaacg ccttcgccac gtactcgacc
     2221 aggtccttgt acaccgggga gtcctccatg gctgctcgca ggtccggcgc ttgccgagta
     2281 cccgcgcccg ggcgcgcccc ggcgggcgtg aagtccgggg gttaaggagt cgccggaggc
     2341 ttattgcgga gctcggcaaa gcgccgcagg acgccggccg tccggagcaa cgtgcggacg
     2401 gtatcggtgg gctgggcccc ccgctcgagc cagcgcaggg ccttatccgc atcgaccttc
     2461 agaccgacag aggtctgccg aggatcgtag gtgcccagac tttccaggaa ccgaccattt
     2521 cgcgccgtcc gttcatcggc caccacgact cggtagaagg gcgtctgttt tgtccccatg
     2581 cgtcgcaacc tgatcttaac cgccatggaa cctcctgaga aatgtgtggg caaccggccc
     2641 cccggcaagg gggcgttggc ccaaccgggg ttagcgagtg gaaaacggca tctgccgcgg
     2701 caggcgtccg cccttgccag cctgcatgag ctgacgcatc atcttctgca tgtccgcaaa
     2761 ctgcttgagc aggcggttca cgtcggccac gttggtcccg gagccggcgg cgatgcgctt
     2821 gcgccggcta ccgttgagga tctcgggttt ctgtcgttcc cccggggtca tcgagtcgat
     2881 gatggcctcg acccgcttca gctctcgctc ttgcgtggtg ctgtcgggca gacccttgag
     2941 ccgcgacagc ccgggaatca tccccagcag ttggtccagg ggacccatgc cccggatctg
     3001 ccggagctgg tcccggaaat cctccagcgt gaaggcctgc gccttgagct tcttctggag
     3061 gaccgccgcc tgcttggcgt ccaccacctg ctcggcccgc tccaccaggc tgaggacgtc
     3121 gcccatcccc aggatgcgag aggccatacg gtccgggtgg aacacctcca gggcgtccag
     3181 cttctcgccc acgccgatga acttgatggg cgccccggtc accgagcgga tggacagggc
     3241 cgcgccgccc cgggcgtccc cgtccagctt ggtgaggatg taaccgctga gggccagggc
     3301 gtgattgaac gccgtggccg agttgaccgc atcctggccg gtcatggcat ccgccaccat
     3361 caggatctcg gtcggtttca cgtccgcctt gatggcgcgg agttcctcca tcagcggctc
     3421 gtccacatgc aggcgcccgg cggtatccag aatcaccgga tccaggaggc gcgatcgggc
     3481 caggctcacg gcctcgcggc agacggcgac agcagagccc gcgctcggat ttcgatacac
     3541 ctccacaccc agggtctccc ccagggtggc gagctgctcc tgcgccgcgg gccgctgcag
     3601 gtcggcagcc acgaggagcg gccgctgccc ctcctgacgg aggagccggg ccagcttgcc
     3661 tgccgtggtc gtcttgcccg acccctgcag gcccaccagc atgaccacgc tgggcggctg
     3721 ccccgcccgt tccagacggc ttgcggttcc acccatcagg gccgtgagct cgtcgtacac
     3781 gaccttgacc acctgctggc cggggctcag gctctccagg acatcgcggc cgaccgcgcg
     3841 ctcccgcact cgctccagga aggttcggac gaccttgacg ttgacgtccg cctcgaggag
     3901 ggccatgcgc acctcacgga gggactcggc gatattgtcc tcggtgagac tgccgtgccc
     3961 gcgaagccgc ttgaagatat cgttcagtct gtcgctaagc ccttgaaaca tggtctcctc
     4021 gcaaaacgca aacaggaaaa gtaccgggat ggcggggatt tgtcaaggtc aaagtccttt
     4081 gggaggccat aagtgacggg aggttagagg ctggcgatgg acagaacata ccacacccat
     4141 gcatacaaac gcaacccagg cgcaaccggg attcggcgtg cactcacgcc cggtgcgcca
     4201 gtccagaagg agggacaact caggcgatga gacaactcct agccgcttag gaaaagggca
     4261 accggcttcc tggaggccgg gatatatgtg ttcctctgac cggggattct tcaagcctca
     4321 ggaacctagc cgatcattca atggaaccaa acactgcgct ttttggttat acagatgaat
     4381 gcaaagaccg cctggcgggg ggccagtgcc gtctgattct tccgggtcct tcaggggggg
     4441 aggaagacat gccttggaat gggacgggga ggtttgccct cggtatcttt accgtgttga
     4501 agcaggcccc accggaatcc gtggtatatg ccctcctctc gggggagagg tgggtggaca
     4561 tcgaggaggc agagaacatt caggttcgcc ttctgacgca cctgaacggc
//
LOCUS       DBNGZC010000420         2008 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1405823, whole genome shotgun sequence.
ACCESSION   DBNGZC010000420 DBNGZC010000000
VERSION     DBNGZC010000420.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1405823"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="AC16G0_07435"
     CDS             complement(<1..989)
                     /locus_tag="AC16G0_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013715.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HIR5099489.1"
                     /translation="MYWISKGFHRLNRFRRRLFIFLAVLGPGIITMVADNDAGGISTY
                     AVTGAKYGFNLLWVFFILVPMAYYVQEMTVRLGAVTKRGHAEAIFEGFGPFWGWFSLA
                     DLAIVNWLTLVTEYIGMTAAMSLFGVPPWLTYLGITGILFSVVLTGKYWTFEKLTLLF
                     CAFNLVYVPAAFWAMKTPTAPGWGQVANGLLVPSLLGGVTADLLFIILANIGTTITPW
                     QIFFQQSAVVDKGLDLHDIQFGKVDTFVGSMVTCIVAAFIIIATGAAFYYHHPPIVIE
                     DAKQTAEALVPLLPHGQGELARRLFAIGLFDAGFLGALCISLSTSWAGGEVFG"
     regulatory      complement(1088..1264)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            1792..>2008
                     /locus_tag="AC16G0_07440"
     CDS             1792..>2008
                     /locus_tag="AC16G0_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="12,18-didecarboxysiroheme deacetylase"
                     /protein_id="HIR5099490.1"
                     /translation="MISISKLYCGGMAESDGLRYGHGGQGPVVSAGAPPASKTAAERR
                     PVTVWNITRTCNLRCIHCYTDSEAKKYS"
BASE COUNT          408 a          618 c          632 g          350 t
ORIGIN      
        1 cagccgaaca cctctccccc ggcccaggaa gtcgacaggg agatgcacag ggcccccagg
       61 aatccggcat cgaagaggcc gatggcaaag agccttcggg ccagctcgcc ctgcccgtgg
      121 ggaagcagcg ggaccagggc ttccgccgtc tgcttggcgt cctcgatcac gatgggcgga
      181 tggtggtaat agaacgctgc gccggtcgcg atgatgatga aggctgccac gatgcacgtc
      241 accatcgagc ccacgaaggt gtccaccttg ccgaactgga tgtcatggag atccagcccc
      301 ttgtccacca cggcgctctg ctggaagaag atctgccacg gcgtgatggt cgtgccgatg
      361 ttggccagga tgatgaagag caaatcggcc gtgaccccgc ccagcaggct gggcaccagg
      421 agtccattgg caacctggcc ccaccccggt gccgtggggg tcttcatggc ccagaaggcg
      481 gccgggacgt acacgaggtt gaaggcgcag aagagcagtg tgagtttttc gaaggtccag
      541 tacttccccg tcagcaccac gctgaagagg atgccggtga ttcccaggta cgtgagccat
      601 ggcgggaccc cgaacaggct catcgcggcc gtcatgccga tgtactcggt cactagggtg
      661 agccagttga cgatggccag atccgcgagg gagaaccagc cccagaatgg cccgaagccc
      721 tcgaagatgg cctcggcgtg gccgcgcttg gtgaccgccc ccaggcggac tgtcatctcc
      781 tggacgtagt acgccatggg caccaggatg aagaacaccc agagcaggtt gaagccgtac
      841 tttgccccgg tgaccgcgta ggtggagata cccccggcat cgttgtccgc caccatggtg
      901 atgatgccgg gtccgaggac cgccaggaag atgaagaggc gacgcctgaa tcgattcagg
      961 cgatggaagc ccttgctgat ccagtacatg aactgctccg aaacagatag gccctttccc
     1021 caagatgggg aaagggccaa agccttcgcc tcggcgttca cctagggcgc caaggcatcc
     1081 agccttcggg attccccact cgtacaagtt ttggcaccgc gcaccgtaag aggccaacgg
     1141 cctcagccac tttgagcaga cccttaatcc gggaatgcct ggtctacccg tcttccaacg
     1201 tctctcgacg ccgccgggca gtggcctgtg tgtcgcgcgg gagcctcacc taccgaggat
     1261 ccgtattctc tctctgatgc tccgagaccc gcatggggtc ccctcatctc agagctcttt
     1321 taggcccgga ctcgttccct gtcaaccccc aaaatgggct ggtcgatagc gtgagcctcc
     1381 caggtgcaaa cgggccgatg tgtccggagg ctcacgttgc aaccgtcgtc ctgagctgcg
     1441 gctcagaggt ctgagcggct cattgccggt ttgtcagata gaggcggggg gcattatcgg
     1501 ataggggatg gcggcccgcc agatgaaggc gggtctccgc tcctacgatc gacccatgcc
     1561 cctcgaccac attcggtgcc acgactcccg gaccagaaag ttgggggaaa tggaaccaag
     1621 actgtcccct ttcctacatg agcgccgaca ggagacaggg gtgcggttgg caacacatga
     1681 gacgtaaagc atttgccgtc ccagccttgc tggaatagga tttgctctca aactgcgcgg
     1741 catggcgcgc gcttacccat tgatgtgcga tgagccgacc aagggggacc gatgatcagt
     1801 atcagcaagc tctactgcgg tggaatggca gaaagtgacg gcctgcggta cgggcatggc
     1861 gggcagggcc cggttgtgtc cgcgggtgcc ccacctgcgt ccaagacggc tgcggaacgg
     1921 cggcctgtga ccgtctggaa catcacccgg acgtgcaatc tgcgctgcat ccactgctac
     1981 acggactccg aggccaaaaa gtacagcg
//
LOCUS       DBNGZC010000421         2422 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1698238, whole genome shotgun sequence.
ACCESSION   DBNGZC010000421 DBNGZC010000000
VERSION     DBNGZC010000421.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1698238"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..155
                     /locus_tag="AC16G0_07445"
     CDS             <1..155
                     /locus_tag="AC16G0_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013551698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HIR5099491.1"
                     /translation="GAKGRMKPLLLAMPVRVILNDKTALLGAARYAALSVTDDPAPAG
                     TRGAME"
     gene            152..1156
                     /gene="fbp"
                     /locus_tag="AC16G0_07450"
     CDS             152..1156
                     /gene="fbp"
                     /locus_tag="AC16G0_07450"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HIR5099492.1"
                     /translation="MSTRAVTLNRHILEEERLHPQAVGELTELLVQLGYAAKILAREI
                     GRAALVGRLGLVGEKNATGDSQKKLDIFANDTVMEAFAQTGLVAGVVSEELDEIKQIS
                     CEGKAKYILCMDPLDGSSNTDINGALGTIFGIYRSARAGGADLMGDILRQKAVPEVAG
                     YVLYGTSTILVYTSGHGVAGFTLDRELGEFLLSHENIRCPERGRTYSANVARMGEWDP
                     DIQRFVRHLTQTDVASGRPYSLRYSGALVADVHRCLIEGGVYFYPPDPGHSQGKLRLV
                     YECAPLAALVEAAGGRASDGRQRVLDIPAETIHQRVPLVIGSAGDVSLYESFLRGETS
                     "
     gene            1153..1968
                     /gene="pgl"
                     /locus_tag="AC16G0_07455"
     CDS             1153..1968
                     /gene="pgl"
                     /locus_tag="AC16G0_07455"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017739784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HIR5099493.1"
                     /translation="MTEKRGATKEARKPEIRILADANLLARTAAEEFAGRAEEAVRVG
                     GSFTVALSGGTTPRALFGLLAGEEDASLRGRLPWGRVQVFWGDERHVPPDHPDSNYRM
                     TVETLLSRVPVPPENIHRIQSEDPDAGKVAEAYEQTLRRSFRLPEGQLPRFDLILLGM
                     GPDGHTASLFPGTPALHERTRLVVAQWVEKLRTHRITLTPPVLNNAALIMFLVSGADK
                     ADTLRAVLLGEYQPERLPAQLVRPTNGRLLWLVDQAAARHLQLSEGSPRPAPD"
     gene            2026..>2422
                     /locus_tag="AC16G0_07460"
     CDS             2026..>2422
                     /locus_tag="AC16G0_07460"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5099494.1"
                     /translation="MRYDAIVIGSGQAGSPLSEDLADHGWSVALIEKAHLGGTCINTG
                     CTPTKTMIASAQVAHYARNALRWGVRTGEVQVDLPRIVARKDEIVQRFRGGKQSAVES
                     RERLHLYRGAARFLDPHRITVQDQVLEAER"
BASE COUNT          412 a          790 c          842 g          378 t
ORIGIN      
        1 tcggcgccaa ggggcggatg aagcccttac tcctggccat gccggtccgg gtcatcctga
       61 acgacaagac tgccctgctt ggagccgccc gctacgccgc cctttcggtc acggacgatc
      121 ccgctcccgc aggcactcga ggagccatgg aatgagcacg cgcgcggtga ccctcaaccg
      181 gcacatcctg gaagaggagc ggctgcaccc gcaggccgtg ggggagctca cggaactcct
      241 ggtccagctc ggctatgcgg ccaagatcct cgcccgcgag atcggtcggg cggctctggt
      301 aggacgattg ggcctggtcg gggagaagaa tgcgaccggc gacagccaga agaagctgga
      361 catcttcgcc aacgacaccg tgatggaagc ctttgcccag acaggactgg tggccggcgt
      421 ggtgtcggag gagctggacg agatcaagca gatctcctgc gaggggaagg cgaagtacat
      481 tctctgcatg gaccccctgg acgggtcgtc caacacggac atcaacggcg ccctgggcac
      541 catcttcggg atctaccgca gtgcccgggc cggcggtgct gatctcatgg gagacatcct
      601 ccgtcagaag gccgtgccgg aggtggccgg gtacgtgctg tatgggacga gcacgatcct
      661 ggtctatacc tccggccatg gggtggcggg attcacgctg gaccgcgagc tgggcgagtt
      721 cctgctgtcc catgagaaca tccgttgccc ggagcggggc cggacctaca gcgccaacgt
      781 ggcccgcatg ggcgagtggg accccgacat ccagcggttt gttcggcatc tgacccagac
      841 cgatgtcgcc agcggccgac cctattcgct gcgctacagc ggggccttgg tcgccgacgt
      901 acaccgctgt ctgatcgagg ggggtgtcta cttctatcca ccggatcccg gccactcgca
      961 ggggaagctc cgcctggtgt acgagtgcgc ccccttggcc gccctggtcg aagcggccgg
     1021 tgggcgggcc agcgacggcc ggcagcgcgt cctggacatt ccggcggaga ccatccacca
     1081 gcgcgtcccc ctggtcatcg gcagtgccgg ggatgtctcc ctgtacgaga gcttcctgcg
     1141 gggggagacc tcgtgacgga gaagcgggga gccaccaagg aggcgcggaa gcccgagata
     1201 cgcatactcg ccgacgccaa cctgctcgcc cggaccgcag cggaggaatt cgccggacgg
     1261 gccgaggagg cggtgcgggt cggaggatcc ttcacggtcg cgctctcggg cggcacgacc
     1321 cccagggcgt tgtttggtct gctcgccggc gaggaggatg cgtcccttcg gggtcggctg
     1381 ccctggggca gggttcaggt gttctggggg gacgagcgcc acgtgccgcc cgaccacccg
     1441 gacagcaatt accgcatgac ggtcgagacg ctcctctccc gcgtgccggt ccctccggag
     1501 aatatccacc gcatccagag cgaagatccg gatgcgggga aagtggcgga ggcctatgag
     1561 cagacgctcc gccgctcctt tcgcctgccg gaaggacagc ttccacgatt tgacctgatt
     1621 ctcctgggga tgggtccgga cggccatacg gcctcgctct tcccgggcac accggccctg
     1681 cacgagcgga cgcgtctcgt ggttgcccag tgggtcgaga aactccggac ccatcggatc
     1741 accttgaccc ctcctgtgtt gaacaacgcc gcgctcatca tgttcctcgt cagcggagcg
     1801 gacaaggccg acacgctgcg ggcggtcctc ctgggggagt accagccgga acggctcccg
     1861 gcgcagctcg tgcgacccac caacgggagg ctcctctggt tggtggatca agcggcggcc
     1921 cggcacttgc agctttccga gggatcaccg aggcccgccc ccgattgaga gcgtgaggcc
     1981 ggtccgcgtt aggcggccag cggcattgcc ggaggaggct ccgagatgag atacgacgcg
     2041 atcgtcatcg gctccggcca ggcgggcagt cccttgtctg aggatctggc cgaccatggc
     2101 tggagcgtgg ccttgataga aaaagcacac ctgggcggga cgtgcatcaa cacggggtgc
     2161 acgccgacca agaccatgat cgcctcggcc caggtggccc actacgccag gaacgccctt
     2221 cgctgggggg tgcggaccgg ggaggtgcag gtggacctgc cccgaatcgt cgcccgcaag
     2281 gacgagatcg tgcagcgctt ccgtggcgga aaacagagcg ctgtggagag tcgcgaacgc
     2341 ctccatctct accgcggggc cgctcggttc ctggaccccc accggatcac ggtgcaggac
     2401 caggtgctgg aggccgagcg ga
//
LOCUS       DBNGZC010000422         3429 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_917009, whole genome shotgun sequence.
ACCESSION   DBNGZC010000422 DBNGZC010000000
VERSION     DBNGZC010000422.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3429)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3429)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3429
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_917009"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="AC16G0_07465"
     CDS             complement(<1..464)
                     /locus_tag="AC16G0_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099495.1"
                     /translation="MRRGWVVAGCLCLAFSWLSPLHAAQSAPMILAVGNTAYLNAPAI
                     QAATGAEVKTDLEKHKLTEFAVIVLANVAYSSLPGAVQQGLVQYVTDGGALLLTGGPQ
                     AFGSGGYQAVAPIVPFQIRSAGDWRVTPFRPPVSLQPGHPILAGVEFITVGAV"
     gene            676..1335
                     /locus_tag="AC16G0_07470"
     CDS             676..1335
                     /locus_tag="AC16G0_07470"
                     /inference="COORDINATES: protein motif:HMM:NF012933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HIR5099496.1"
                     /translation="MRPAIKVTRVAALLRKSYGPRRQQRRRDNPLDSLIYTLLSQNTS
                     DLNSERAYANLRRRFPAWEDVHRASLRELIGAIRSGGLANIKAVRIKALLEEIWGDQG
                     HFDLSFLRDLPDEEAKAYLRQFKSISSKTIDCVLLFGLGRPSFPVDTHVFRVSRRLGL
                     LNGLPTPEKAQEFLEPRIPSGDRYALHLHLVEHGQKVCKPQRPLCEDCVLARLCDHVH
                     K"
     gene            1483..1556
                     /locus_tag="AC16G0_07475"
     tRNA            1483..1556
                     /locus_tag="AC16G0_07475"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1515..1517,aa:Gly,seq:ccc)
     gene            1651..2265
                     /locus_tag="AC16G0_07480"
     CDS             1651..2265
                     /locus_tag="AC16G0_07480"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR5099497.1"
                     /translation="MDRVPVSLKDRVAWALHGRLAVQAAPEGFRRAAVLIPLYESEAG
                     PHLVLTKRTESVPTHKGQISFPGGGFEETDRNFRTTALREAEEEIGLRPQDVEILGTL
                     DDTVTASSRHVVRPFVGIVPHPYPYRLDPFEIESLIHLPIRALLSGAPFREETWERDG
                     RQFPVYFYEHEGQTIWGLTARILKQFVEVVGAASFEGGLAPSGE"
     gene            2558..>3429
                     /locus_tag="AC16G0_07485"
     CDS             2558..>3429
                     /locus_tag="AC16G0_07485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5099498.1"
                     /translation="MRGIAIARTGREHDPSLGSPKRLAFVTSTPLSVAGGSGTYVGIR
                     QLARSLEDRGIRVEVHAPTFWLPSFTVKRLLFNLLVAPRLRRATCDWVVGFDLDGFQY
                     GRSKVAPYVASIKGVIADELQNERGVVRAMLGIQAAFERLAVRRADVVVATSLYSRDR
                     IVQAYGISPAKVRIVPESIDLLTWAAGQVAPVEMASAPPAILTVAHMYPRKNLGALLE
                     AYAHLQAAGIPFQGWIVGEGPCRTAWERLRDTLGLAERVKFLGTIPRRELEDRYRRAA
                     IFCLPSRQEGFGIV"
BASE COUNT          598 a         1080 c         1112 g          639 t
ORIGIN      
        1 acggctccga ccgtgatgaa ttcgactccc gccaggatgg ggtgaccggg ttggaggctc
       61 accggcgggc ggaaaggggt cacgcgccag tcgccggcgg atcgaatctg aaatggcacg
      121 atgggggcca cggcctggta cccgccgctt ccgaaggcct gagggccgcc ggtcagcagc
      181 agggcgccgc catctgtcac gtactgcacg agtccctgtt ggaccgctcc tggcaacgag
      241 gaataggcca cgtttgccag cacgatgacg gcgaactccg tcagcttgtg cttctccagg
      301 tcggtcttga cctcggcgcc ggtggcggct tggatggctg gcgcgttgag gtaggcagtg
      361 tttcccacag ccaggatcat tggggccgac tgggctgcgt ggagaggcga caaccagctg
      421 aaggcgaggc acagacagcc agccacgacc catcccctgc gcatcgttcc ctcctcgcgt
      481 gtgagtgaac tggctcgcac tatacacgcc cggaccggcg aagtaaaggg agcgcccggt
      541 cgtcatgggc cgtgtcatca cccgcccgca tggccggata cgcgagggtc gctccgtccg
      601 tccttttgga ttttcgcgtt gacattcctc cagcccttcg ttagcgtagc gacctgtcgt
      661 ccaactgagg acaacatgcg cccagcaatc aaggtcacgc gtgtcgcggc gttgctccga
      721 aaatcgtatg gtccccgccg ccagcaacgc cgacgtgaca acccgctgga ctccctcatc
      781 tacacgctgc tctcccagaa caccagtgac ctgaacagcg agcgggcgta tgcaaacctg
      841 cggcgtcgtt tccccgcatg ggaggatgtg catcgcgcct ccttgcggga attgattggg
      901 gccatccgga gcgggggctt ggccaacatt aaggcggtcc ggatcaaggc gctcctggaa
      961 gagatctggg gagaccaggg gcacttcgac ctcagcttcc ttcgcgatct gcctgacgag
     1021 gaggccaagg cctatctgag acagttcaag tcgatcagca gcaagaccat tgattgcgtc
     1081 ctgctcttcg gcttggggag gccgtccttc cccgtggaca cccacgtctt ccgggtgtcc
     1141 cggcggctgg gtctcctgaa cgggctgccc acgccggaga aggcccagga gttcctggaa
     1201 ccccgcatcc catccggcga ccgctatgcc ctgcacctgc atctcgtgga acacgggcaa
     1261 aaggtgtgca agccacagcg tccgctgtgt gaggattgcg tgctggctcg tctctgcgac
     1321 cacgttcaca agtaggatga aagcgagggg cacgagggga agggtgattc gggacgctgg
     1381 cttccagaac cagttgccca taggcttgcc catccgccgc gaattgagcg cgcctttctg
     1441 ttgacagcgg cagggcaatc tggtaggaaa acggagcaat ctgcgggtat cgtccaatgg
     1501 tagggcacag gcttcccaag cctgggatgc gggttcgatc cccgctaccc gctccaagga
     1561 tggtgatgaa ggggcttcgg ccaacaccga ggccccttgt gcttttccgg cggatgcatg
     1621 ccgggattgg ccggaggggg tgagggatcg gtggatcgcg tgcctgtcag cctgaaggac
     1681 cgggtggctt gggccctcca cggacggctc gccgtccagg cagcccccga aggattccgg
     1741 cgcgctgccg tcctcatccc cctgtacgag tcagaggcgg gaccgcacct tgtcctgacc
     1801 aagcggacag aatcagtacc gactcacaag gggcagatca gcttccccgg ggggggattc
     1861 gaagagacgg acaggaattt tcgcaccacg gccctccgcg aggccgagga ggagattgga
     1921 ctacgtccgc aggatgtcga gatcctgggg accctcgacg acaccgtcac cgccagtagt
     1981 cgccacgtcg tgcggccctt cgttggaatc gttccccacc cctacccgta tcgcctcgat
     2041 cccttcgaga tagagtctct cattcacctt ccgatccgtg cgctcctctc tggggcaccc
     2101 tttcgcgagg agacgtggga gcgggacggc cgccagtttc ccgtctattt ctacgagcat
     2161 gagggtcaaa ccatctgggg cctgaccgcc aggattctga agcaatttgt tgaagtggtc
     2221 ggagctgcct ctttcgaggg cgggctcgct ccctccgggg agtagccgcc gatcgccagc
     2281 cgtggacttc aaatctctgg aaataccgag acgaatgaat ccgtgtgcac gtagtcatgg
     2341 gcgctgcttg caggaggtca gcgatcggtg gttgcggccc gagtctgtgc ctacggcagc
     2401 ctccccggag gggcttttcc cttgacaccc ctccgatggc ggattagcgt gagccggttt
     2461 tcacggcagg atcgcggccg cgcgtgagcg gaatgaagat ctgattcgcg ggcgtccgat
     2521 aaggtccccg aacccctcac tgatgcggaa tagacgcatg cgtggcatcg caatcgctcg
     2581 aacgggtcgg gagcacgatc cgtctctcgg cagcccaaag cggctggcgt tcgtgacctc
     2641 cacacccctg agcgtagccg ggggaagcgg cacctacgta ggcatccggc agctcgcccg
     2701 gtccctggag gaccggggga ttcgggtcga ggtccacgcg cccaccttct ggctgccgag
     2761 cttcacggtc aagcggctgc tcttcaacct cctggtggcg ccacggctcc gaagggccac
     2821 gtgcgattgg gtcgtcgggt tcgatctgga tggatttcag tatggccgat ccaaggtggc
     2881 tccctacgtt gcctccatca agggggtcat tgccgacgaa ctccagaacg agcgcggcgt
     2941 cgtgcgggcg atgttgggca tccaggcggc cttcgaacga ctggcggtcc gccgcgccga
     3001 tgtggtcgtg gccacgagcc tgtactctcg cgaccgcatc gtccaggcgt acggaatatc
     3061 accggcaaag gtgcggatcg ttcccgagtc gatcgatctt ctgacgtggg ccgcgggcca
     3121 ggtagcgccg gtcgagatgg cttcggctcc ccccgcaatc ctgactgtgg cccacatgta
     3181 tccacgaaag aatctcggcg ctctcctcga ggcttacgcg cacctgcagg ctgcgggtat
     3241 tccgttccag ggatggatcg tgggcgaggg gccctgccgc accgcctggg agcgcctgcg
     3301 ggatacgctc ggtctcgcag aacgagtcaa gttcttgggg accattccgc gccgcgagct
     3361 cgaggatcgc tatcgtcgag ccgccatctt ctgcctgccg agccgacagg agggattcgg
     3421 gatcgtctt
//
LOCUS       DBNGZC010000423         5395 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1013526, whole genome shotgun sequence.
ACCESSION   DBNGZC010000423 DBNGZC010000000
VERSION     DBNGZC010000423.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1013526"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..849
                     /locus_tag="AC16G0_07490"
     CDS             184..849
                     /locus_tag="AC16G0_07490"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5099499.1"
                     /translation="MTIRLARDARLVRFPSSDGLQIEGRLTRRDRERAVVLCHPHPLH
                     GGSMLTPVIMTAEQIFQESGYTTLIFNFRGVGASEGAYGEGLAEVADVTGGLSFLEET
                     LGGPPRLQAVAGYSFGSFVGGSVAASDPRVRLYLGIAPPLNAYDFGFLKAAMCAVALI
                     GAKRDEFCDVTELQSFYLRLPATPWLRLLDTDHSFGDAMDELAQACKDVVSWVGETQR
                     RVP"
     gene            complement(893..1393)
                     /locus_tag="AC16G0_07495"
     CDS             complement(893..1393)
                     /locus_tag="AC16G0_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099500.1"
                     /translation="MRRTGKRLVPCMLLALPLVLALPGGAASGPAATPEAGPLQVNGS
                     LPAPAGFIQASNCVAKEGIHYVKEGERTLMPILAYDEAGKLVSLEYIIARKALEAGSS
                     WKGLPGVAGRAIDHIDVDYYPGSRKGTSTPYYSIHLYFIDGKEQQQICPKSTAVEPVG
                     RVIRSQ"
     gene            complement(1828..3036)
                     /locus_tag="AC16G0_07500"
     CDS             complement(1828..3036)
                     /locus_tag="AC16G0_07500"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5099501.1"
                     /translation="MSNAIGSKEENAPLWDATFGWFLVVFFLFNVGLGVFPPLLPQIM
                     EDLGMTFAATGLLGSAYALFRFFVDLPAGILAERLGIPLLMHGAVGLLLAGTVLSVWA
                     DGFWVMLAARSLLGVGSGMAMVFAILYLMRRGPAGQRNRRANLYEASVIAGMAISSQM
                     GGLIASRWSWRWSFGAGAVVLVLAWAVMMWALLPGVRDLMQGVGETVEVAHRQRSAES
                     PGPTFTIYLLIFGQAFVWGGGISTLLPLYGGDTLHLTPEAIGRTMAVAFWIEVCLLFP
                     VGWAADVLGKVRVVVPGFLAMLVGIMCVPLAQGVWSYGVTFAFLVSGMSVWMAVPALH
                     AERLAGGFRGKAAGIYRLVTDLGFIAAPALVGWLIGQYGFVAGAFPMAVVLIASILMT
                     LCFLRARPSA"
     gene            complement(3140..4330)
                     /locus_tag="AC16G0_07505"
     CDS             complement(3140..4330)
                     /locus_tag="AC16G0_07505"
                     /inference="COORDINATES: protein motif:HMM:NF046806.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5099502.1"
                     /translation="MDVVRIGMVGARFGATLHMQNLIPMRGREAEVVGVCARSHESAQ
                     AFAQRFEIPFVTTEFRALLDRKDVDAVDLCVPPSVHHSFAIQAAEAGKHVIIEKPLTG
                     YFGEPGDPEPIGEKVARTKMLEGARKNARAVQEAVRRNKVKLGYAENWVYAPPVAKLR
                     RLIRAAGGSILDVRAEECHSGSHSTFAKQWRFAGGGALLRMGVHSVGACLHLKATEGK
                     IRDGRPIRPISVTADAASLMRLSTARRGPRRWIAADPDDVENWANLVLAFEDGTRGLV
                     TVSDAGLGGLNTKIGVYMTNAVLHTNMTQNTAVQAYAPDPTIFGEEYFTEKLETKAGW
                     TFPSPDEDWFRGYPQEMRDFVECIRENREPLAGVDLAVDCVEVIYAAYLSAAEGRRVV
                     LAGR"
     gene            complement(4535..5032)
                     /locus_tag="AC16G0_07510"
     CDS             complement(4535..5032)
                     /locus_tag="AC16G0_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099503.1"
                     /translation="MDDFSLDKTKLLEAVIGGVEETLRTMDSGYAAARQATLDSPHVM
                     KSKREVMGIEASYLANGLAQNIQEREHELRTLRSLHLPEAPSRVSLGCVVGVGPPEEP
                     VESLYFLLPVCGGMAVPLEGGERTVRVLTPGAPVARALLGKRLGEDVALPLHPAQPTC
                     VRFLA"
BASE COUNT          985 a         1831 c         1637 g          942 t
ORIGIN      
        1 gggaggcgaa gcgggggagc tgaaggtgga cgtctggcgt ggacctcgcg agagaggaat
       61 gagcgtttcg gaaactgacg gaatgacgta tgatccggct atgcggtgaa caccctccaa
      121 cagccgcgcg cctgccgctg ccatggacag cgccatgatt ccgacaggcg atggagttct
      181 gcaatgacga ttcgattggc tcgggatgcc aggctggtcc gtttcccctc ctccgacgga
      241 ttgcagatcg agggacgcct gacccggcgc gaccgggagc gggctgtggt cctgtgtcac
      301 ccccacccgc ttcacggcgg atccatgctc accccggtca tcatgaccgc cgagcagatc
      361 ttccaggaga gcggctatac caccctgatc ttcaacttcc gcggggtggg ggcgagcgaa
      421 ggcgcctacg gagagggcct cgcagaggtc gccgacgtca cggggggcct cagcttcctc
      481 gaggagaccc tgggcggccc acccagattg caggccgtgg ccggatactc cttcggcagc
      541 ttcgtgggag gatcggtcgc cgccagcgat cctcgtgtca ggctctacct cggcatcgcg
      601 ccacccttga acgcgtacga ctttggattc ctcaaggcgg cgatgtgtgc cgtcgccctg
      661 atcggggcaa agcgagacga attctgcgac gtgacggagt tgcagtcatt ctatctccgg
      721 ctgcccgcaa ccccctggct tcgtctgctg gacaccgacc attccttcgg cgatgccatg
      781 gatgagctgg cgcaggcctg caaggacgtc gtgtcctggg tgggtgagac ccagcgaagg
      841 gtcccctgat ggggcaagcc atccctcttg cgctgaccgg ccgccggggg tcttactggg
      901 atcgaattac gcgacccacc ggctccaccg ccgtactctt ggggcagatc tgctgctgct
      961 ccttcccgtc aataaagtac aggtggatac tgtaatacgg agtcgaagtc cccttgcgac
     1021 tgcctggata gtaatccaca tcgatgtggt cgatggcccg gccagccacg cccggcagcc
     1081 ccttccacga gctaccagcc tcgagggcct tccgagcaat tatgtattcg agggagacca
     1141 gttttccggc ctcgtcatac gccagaatgg gcatcaaggt cctctctccc tccttcacgt
     1201 aatggatgcc ctccttggcc acacaatttg acgcctggat gaaacctgcg ggcgccggca
     1261 gcgacccgtt gacctggagc ggaccggcct ctggagtagc cgcaggaccg gaggccgcac
     1321 cgcccggaag ggcaaggacc aggggaaggg caagcaacat acaggggaca agcctctttc
     1381 ccgttcgtcg catctctgtc ctccttctac ttccttcccg cctcaacccc ggcgcccgtt
     1441 ctccccggtc gtgtcacggt tctctgcgcc agtccgtcct cgtcttgtac ttccggagcc
     1501 gtccggttga aaccctccgc aaatctgtct agtccccgga gtgcgcgcct acacccgcca
     1561 actcggggac atgaggcgga ggccgcctca ggtatctggg atgcataccg gacatatcct
     1621 ctttgcaacg cctgtgccga ggatctccgg aatagggtta cttcaggacg gtctctgcat
     1681 ttgccctcta ttgctgccat cacgggtagg tcgaagccgc cagactcctc ccgcaagtcc
     1741 atccctcctt gtggcaacag ggacttcgcg gtgtgtcacc tcccgcgttc atctgttcca
     1801 ttgttgggca gcagcatctc gggtgcgtca agcggagggc cgtgctcgga gaaagcagag
     1861 ggtcatgagg atggaggcta tcagaaccac agccatcgga aaggcgcccg ccacgaagcc
     1921 atattgcccg atcagccaac cgacgagcgc cggggccgcg atgaatccaa gatccgtcac
     1981 cagccggtag attccggcgg ccttcccgcg gaacccccct gcaagccgct ccgcgtggag
     2041 cgcgggtaca gccatccaga cgctcatccc gctgaccagg aaggcaaagg taacgccata
     2101 gctccagact ccctgcgcca ggggcacaca catgattccg acgagcattg ccaggaaacc
     2161 tgggaccacc acccggacct ttcccagaac gtccgccgcc cagcccacgg ggaaaaggag
     2221 gcagacctct atccaaaagg cgacagccat ggtccgtccg atggcttccg gcgtcaggtg
     2281 aagggtgtcc cctccgtaga ggggaaggag cgtggaaatt ccgccgcccc acacgaaggc
     2341 ctggccaaag atcaacaggt agatggtgaa ggtgggtccc ggagactccg cggaccgttg
     2401 gcggtgcgca acctcgaccg tctcgccgac cccttgcatg agatcccgga ccccgggaag
     2461 cagcgcccac atcatcacag cccaggccag cacgaggacc acggcgcccg cgccgaagct
     2521 ccaccgccaa ctccagcgac tggcgatgag tccgcccatc tgcgagctga tggccatgcc
     2581 cgcgatgacc gacgcctcgt acagattcgc ccgacggttg cgctggccgg cgggcccccg
     2641 gcgcatcagg tagaggatcg cgaagaccat ggccatgccg gacccgaccc ccagcaggct
     2701 ccgcgcggcg agcatgaccc agaacccatc cgcccagacg gacaagacgg tgccggcgag
     2761 aagcagtccg actgcaccgt gcatcaagag ggggatccca agccgctccg cgaggatccc
     2821 cgccgggaga tcgacgaaga accggaagag ggcgtaggcc gaccccagca aaccggtggc
     2881 agcgaaggtc atgcccaggt cttccattat ctgcgggagg agcggaggaa acacgcccag
     2941 gccgacgttg aagaggaaga agaccacgag aaaccacccg aaggtggcat cccaaagggg
     3001 ggcgttctct tccttcgacc cgatcgcgtt tgacatctct ggacacccgg agacccgcga
     3061 atcgatggac ggagtcatga cgcggcaagc cctctcatgg tccggcgaca cctccggtga
     3121 acctgtccgc tgggtatcct cagcgaccgg cgagcaccac tcgccgcccc tcggccgcgg
     3181 acaggtaggc cgcgtagatc acctccacac agtccacggc taagtccacg ccggccagtg
     3241 gctcgcggtt ctcccggatg cactccacga aatcccgcat ctcctgcggg tagccgcgaa
     3301 accaatcctc gtcgggactg gggaacgtcc agcctgcctt ggtctcgagc ttctccgtga
     3361 agtactcctc cccgaagatc gtcgggtcag gcgcataggc ctgcacggcg gtgttctgtg
     3421 tcatgttggt gtgcaggacg gcattcgtca tgtacacgcc gatcttggta ttcaacccac
     3481 ccagtcccgc gtcgctcacg gtgacgagac cgcgggtccc gtcctcgaat gccaggacga
     3541 ggttggccca gttctccaca tcatcgggat ccgcggcgat ccaccttcgc gggccgcgcc
     3601 gggcggtcga aagtcgcatg agcgaggccg cgtccgccgt caccgagatc ggtcgtatgg
     3661 gtcgcccatc gcggatcttc ccttcggtgg ccttgaggtg gaggcacgcc ccgaccgagt
     3721 ggacccccat gcgcagaagt gccccgcccc cggcgaacct ccactgcttg gcaaaggtgg
     3781 agtgagaacc cgagtggcac tcctcggctc gcacgtccag gatgcttcct cccgcggcgc
     3841 gaatgaggcg ccggagcttc gcgaccggcg gggcatagac ccagttctcg gcgtagccga
     3901 gcttgacctt gttccgccgg actgcctcct gcacggcgcg agcgttcttc cgggctccct
     3961 ccagcatctt ggtccgtgcg accttctcac cgatcggctc ggggtcaccc ggttcaccga
     4021 agtagccggt gagcggcttc tcgatgatga catgcttgcc ggcctccgcc gcctggatcg
     4081 cgaagctgtg gtgcaccgac ggggggacgc agaggtctac ggcatccaca tccttccggt
     4141 cgaggagcgc ccggaattcc gtggtgacga agggaatctc gaaacgctgg gcgaaggcct
     4201 gggcgctttc atggctcctg gcgcaaaccc cgacgacctc cgcttcccgg ccccgcatgg
     4261 ggatgaggtt ttgcatgtgg agcgtcgcac cgaagcgcgc ccccaccatt ccgatccgga
     4321 cgacatccat cttccctccc cttccattcg acaggtccaa tgcacacgcc ggcccgctac
     4381 agtctcggtg tccacgccgg agcgctcgcc gacatggcgc ttcgcaggat gccgaagaac
     4441 tcgtttcgcc caggctgctc aaagggttca gatgcaaggc ggcgcaccca tccttcgatg
     4501 ggcacccggc agatgggcct tgttcagcag cctgctacgc aaggaaacgc acgcaggtcg
     4561 gttgggccgg gtggagcgga agcgccacat cctcccccag acgcttgccc agcagcgctc
     4621 gtgccacggg tgccccggga gtgaggacgc ggacggtgcg ctcgccgcct tccaggggaa
     4681 ccgccatgcc accgcagacg gggaggagga aatacaggga ctccaccggc tcctccgggg
     4741 ggcccacgcc gaccacgcag cccagcgaga cccgcgaggg ggcctcgggc aggtggagac
     4801 tcctgagcgt ccgcagttca tgttctcgtt cttgaatgtt ctgcgccagt ccgttcgcga
     4861 ggtatgaggc ctcgattccc atgacctcac gcttgctctt catcacgtgg ggcgaatcca
     4921 gggtggcttg cctggctgca gcatagcccg agtccatcgt ccgcagcgtc tcctccaccc
     4981 cgccgatgac ggcctcaagg agtttcgtct tgtccagact gaagtcgtcc atgcctcttc
     5041 gtccatctcc gtcgtgatcc ccttcggttc cccgcaagga tacagaagcc gcctcctcgc
     5101 atcaagtaca gagcgccgat ctcttgtccc gtgccgctct acgaggcgtt cttgcttgac
     5161 agtgcatcca cggccgccta gactccgccc ataccggacc cggggactcc gcattgcata
     5221 gcctgtgaat cctttcggtc atactgtggt cctcgtggta actcccgacg cccgctaacc
     5281 ttgttgaaaa gccaggatga tcctagcgtg tttaggccat cggatgatct ggctcctcgg
     5341 attcaaaccg cacaacgtga ccgcagcctg acggcgagtc ttcggcaagg gtttc
//
LOCUS       DBNGZC010000424         2233 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_917238, whole genome shotgun sequence.
ACCESSION   DBNGZC010000424 DBNGZC010000000
VERSION     DBNGZC010000424.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_917238"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="AC16G0_07515"
     CDS             <1..663
                     /locus_tag="AC16G0_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099504.1"
                     /translation="RGVAVYVSIVTEEIYEYEVCFKCHGIQDQTTPGQVRQDNSRNIR
                     LLLNPSNPSYHPVITAGKNPTIQGLEPGYTASSLIYCTDCHNNDEWTSTGIKPKGPHG
                     SRYQPILEREFDQIDPNPESFPAYALCYKCHNQTSLLGSGRFPHRLHVVDKQASCTVC
                     HNAHGSRQNPHLINFLLRDRLNKVVVSTNGKGLIQFVSTTPGHGSCSLRCHGTDHDQK
                     SY"
     gene            948..1316
                     /locus_tag="AC16G0_07520"
     CDS             948..1316
                     /locus_tag="AC16G0_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5099505.1"
                     /translation="MSCRVIILYTNALFGHGLRSLLARQGGFEVPVVAAKDADLATAV
                     REHRPDVIIVEGEGLSPENARALLEAAWGEPRVRVVNIRNDAKQPTVWRAVTIVPEGQ
                     ESAADALERILTVGADPAER"
     gene            1338..>2233
                     /locus_tag="AC16G0_07525"
     CDS             1338..>2233
                     /locus_tag="AC16G0_07525"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5099506.1"
                     /translation="MKDPASMFHVAVPDYGYWRRQIKCQDACPVHTDARGYVSAIGDG
                     DPERAYIIAREPNPFASVCGRVCAAPCEVACRRGDIDAPIAIRALTRFVTERYGVEAP
                     LRADRDRPAVEATGLPLVESAAAVREIRNVEDGTGLRALGRLAHPGKGKRVAVVGSGV
                     AGLTCAHDLTLLGYRVTVFEKQSVPGGMLMLGVPEYRLPRDLVRAEIQAVLALGVELR
                     ANSAIGRDFSLGDLRGQGFEAIFLAIGCHKARGLPIEGTGLDGVFRAVEFLLNVNLGF
                     KVDLGQRVIVVGGGDVAMDAART"
BASE COUNT          407 a          734 c          722 g          370 t
ORIGIN      
        1 cggggggtcg ccgtgtacgt gagcatcgta acggaagaga tatacgagta cgaggtctgc
       61 ttcaagtgcc atggcatcca ggaccagacg accccggggc aggtgcggca agacaacagc
      121 cgaaacatcc gcctcctgct gaaccccagc aatccctcct atcatccggt gatcaccgcc
      181 gggaagaatc cgaccatcca gggcctggag ccgggctaca cggcctccag cctgatctac
      241 tgcacagact gtcataacaa cgacgagtgg acatccaccg ggatcaagcc aaagggtcca
      301 cacggctcgc gctaccagcc catcctggag cgggagttcg accagatcga ccccaaccca
      361 gaatcctttc ccgcgtacgc cctctgttac aagtgccaca accagacctc gctcctcggg
      421 agcggccgat tcccgcatcg gctgcacgtg gtggacaagc aggcttcctg caccgtctgc
      481 cacaatgctc acggatcgcg gcagaatccc cacctcatca acttcctgct gcgggaccgt
      541 ctgaacaagg tcgtggtgag caccaacggc aaaggcctca tccagttcgt ttccacgacg
      601 cccggccacg ggagctgttc gctccggtgt cacgggacgg atcacgacca gaagagctac
      661 tgacccgaag ttgtacgata tcccacacag gggtcgagtg ctcatcctgt cacgacccac
      721 acaacaaggc gtccgtagag acgacgttcc ttctccctcc acggaatcca tctcccaaca
      781 ggccccgatg tgggcggcca cctgaatcct ctcgccctcc gactcgtatg acccgaaagg
      841 gccatacccg atggcccttc ttcttcgcga gaatggcccc accgcaggat ggtcaaccgc
      901 cttgtgccgg aggatactca accacagagt ggcgagaggg aaatcccgtg agctgtcggg
      961 tcatcattct ctacaccaac gccctcttcg gccacgggct gcgcagcctg ctggcccggc
     1021 aagggggatt cgaggtcccg gtcgtagcgg ccaaggatgc cgacttggcc accgccgtcc
     1081 gggagcaccg cccggatgtg atcattgtcg aaggtgaagg gctctcgccg gagaatgcgc
     1141 gggcactcct ggaggcggca tggggcgagc cgcgagttcg ggtggtgaac atccggaatg
     1201 acgcgaagca gcccactgtg tggcgagcag taacgattgt cccggaaggg caggagtcgg
     1261 cggccgatgc cctggagcgg atactcacgg ttggcgccga ccccgccgag cgatagtcgc
     1321 gaggagaatc cgagaggatg aaagaccccg ccagcatgtt ccacgtcgcc gtgcccgact
     1381 acgggtactg gcgccggcag atcaagtgtc aggacgcctg tcccgtgcac acggacgcgc
     1441 ggggctacgt ctcggccatc ggcgacgggg acccggagag agcctacatc atcgctcggg
     1501 agcccaaccc cttcgcctcc gtctgcggcc gggtgtgcgc ggccccgtgc gaggtagcct
     1561 gccgccgggg agatattgac gcgcccatcg ccatccgcgc cctcacgcgg ttcgtgaccg
     1621 agcgctatgg ggttgaggcg ccattgcggg ccgaccggga ccgcccggca gtggaagcga
     1681 ccggtcttcc gctggtggaa tcggccgcgg ccgttcgcga gattcggaac gtcgaggacg
     1741 gcacgggtct gcgcgcgctc gggcggctgg cccatcccgg aaaggggaag cgcgtggctg
     1801 tggtgggttc cggggtggcg ggtctcacct gcgcccacga tctcacgctt ctcggctacc
     1861 gggtcacggt cttcgagaag cagtcggtgc cgggtggcat gctcatgctt ggcgtccccg
     1921 agtaccgcct tcccagggac ctcgtccggg ctgagatcca ggccgttctg gccctggggg
     1981 tcgaactccg cgcgaacagc gccattggcc gtgacttctc cctgggagat ttgcggggcc
     2041 agggtttcga ggccatcttc ctggccatcg ggtgccacaa ggcgcgcggc cttcccatcg
     2101 aggggacagg cttggacggc gtctttcggg cggtggagtt tctcctcaac gtgaacctcg
     2161 gattcaaggt ggacttgggg cagcgggtga tcgtggtggg gggaggcgac gtcgccatgg
     2221 acgcggcccg cac
//
LOCUS       DBNGZC010000425         4245 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1307527, whole genome shotgun sequence.
ACCESSION   DBNGZC010000425 DBNGZC010000000
VERSION     DBNGZC010000425.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1307527"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="AC16G0_07530"
     CDS             <1..498
                     /locus_tag="AC16G0_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR5099507.1"
                     /translation="IEQVLHLDAELERLAHQYHTKTDFLYLARGINYPIALEGALKLK
                     EISYIHAEGSPAGEMKHGPIALITDEMPVVILAPKDKVYEKILGNIQEVKARDGIVIA
                     VCSEDDQELPRKVDHCLIVPRTSRLLTPALLAIPMQLLAYHIGVHKGCDVDQPRNLAK
                     SVTVE"
     gene            849..1790
                     /locus_tag="AC16G0_07535"
     CDS             849..1790
                     /locus_tag="AC16G0_07535"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009113862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR5099508.1"
                     /translation="MITLEDVKQAAGRIPPFVRRTPLLPARCLKDNPYPEGELLLKLE
                     CLQATGSFKARGAANKLLSLSDTEIRRGIVTASGGNHGLAVAYVGWLGKTQTTVFLPD
                     NASPEKRKKIQHWGATVRIEGHHWHEANRAALALAEREGLTYFHPFADPVVIAGQGTT
                     ALEILEQAPDVEEILVAIGGGGLISGISLAAKALRPSIRITGIEPTGSPTLHECLKAG
                     QVVELREITTIVPTMACAKTEPINYEIVRQYVDEIVLVTDDDMREAAQWLWFEMGIAT
                     DLSGAATIAALRSGKVRPAKGRNVCALVCGAGPDGMQ"
     gene            1824..2036
                     /locus_tag="AC16G0_07540"
                     /pseudo
     CDS             1824..2036
                     /locus_tag="AC16G0_07540"
                     /inference="COORDINATES: protein motif:HMM:NF022018.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            2218..3549
                     /locus_tag="AC16G0_07545"
     CDS             2218..3549
                     /locus_tag="AC16G0_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR5099509.1"
                     /translation="MKWRKDRDQSWVSKESLHVYGPMLVLTLVGFVVAFHFVKPAPPT
                     HLVIATGRTDGAYYQFALRYRELLARRGIQLEVRETSGSVENIQLLEDPSKGVDVAFV
                     QGGTGGTARSGELVSLASLYFEPLWVFTRASLGAEDMSGLRGRRLAVGPEGSGTRAIA
                     ELLLGANGIATDSTFLLPLAGLEAAQALQAGGVDAAFFVASPQSAILREAVRLQGVAL
                     MSFQRADAYTRLYPFLTKLVLPRGTLNLPADLPPRDTLLLAPAASLVVRNDFHPALVD
                     LLLGAATEVHGGAGMFERALQFPSAENLEFPLSSEARRYFRTGPSFLARYLPFWAATL
                     VDRMKVMLLPLITLLIPLFKVVPPTYRWRVRAKIYRWYRDLHAVDTALAERPKGGDLS
                     DLLRELDRIEAEVLRIVIPLSYSESHYNLRLHIEWLRSEVRAAQSTLKEGA"
     gene            3599..>4245
                     /locus_tag="AC16G0_07550"
     CDS             3599..>4245
                     /locus_tag="AC16G0_07550"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5099510.1"
                     /translation="MPPVGIAIVGCGGMIAGVHAAALKEIPDARLVGAWSRSPEKTQR
                     FSDQHGVRAYRSHEELLGDPAVQAVSICLPSGHHAEYGVKAAAAGKHVIVEKPIDISI
                     PKAMSLIEVCRKNGRRLSVIFQNRYTPAAKKVRKALDQGLLGRLILGDAYIKWYRSPE
                     YYKSNAWRGTKAIDGGGALINQAIHTIDLLQWMMGGVQRLNGLVRTSTHAIESEDL"
BASE COUNT          764 a         1355 c         1372 g          754 t
ORIGIN      
        1 atcgagcagg tcctccatct ggacgcagag ctggagcgac tcgcgcacca gtaccacacc
       61 aagaccgact ttctgtatct cgcgcggggc atcaactacc ccatcgccct ggagggtgcc
      121 ctgaagctga aggaaatctc ctacatccac gcggaagggt cccctgccgg cgagatgaag
      181 cacggtccca tcgccctcat caccgacgag atgccggtgg tgatcctggc gccgaaggac
      241 aaggtgtacg agaagatcct ggggaacatc caggaggtca aggcgcggga cgggatcgtc
      301 atcgccgtct gctcggagga cgaccaggag ctgcccagga aggtggacca ctgcctgatc
      361 gtgccccgca cctcccgcct gctcacgccc gccctgctcg ccatcccgat gcagctcctg
      421 gcctatcaca tcggcgtcca caaaggctgc gacgtagacc aaccccgcaa cctggccaag
      481 agcgtgacgg tggaatagcg gtgccgggga tcatttgcca ggtaaaggcg ccgcctcaag
      541 cgactcgcgg gactcgctcg tctgccaaga gcctgtgttt cccgtgtccc gatctgcgta
      601 aggagcatgc tctggtattc tgaccgctgg aatacttgca gaggtttcct tcactctgga
      661 agcttccagg gtccatccgc tcggtggcca ctccccgacc cctccccgat gtctgacggc
      721 cgtcaccaag gcagcctccg tcttgacacg accgaatggc gctgataacg tccggtcacg
      781 agatgactcg cgcagtggcc acggagcgct gccttcctct cctcgccagt tcagccggag
      841 acccagacat gatcacgctc gaggatgtga agcaggcggc tgggcgcatt ccgccctttg
      901 tcaggaggac gccgcttttg cccgcccgat gcttgaagga taacccctat ccggagggag
      961 agctcctctt gaagctggag tgcctccagg ccacgggctc gttcaaggcg cgcggggctg
     1021 ccaacaagct cctgtccctc tccgacacgg aaatccgccg cgggatcgtc acggcctccg
     1081 gggggaacca tggtctcgcc gtcgcctacg ttggctggct ggggaagacc cagaccacgg
     1141 tgttcctgcc cgacaacgcc tcgccggaga agcggaagaa gatccagcac tggggagcca
     1201 cggtgcggat cgaggggcac cactggcatg aggccaatcg ggcggccctg gcgttggcgg
     1261 agcgggaggg actcacctat ttccatccct tcgccgaccc cgtggtgatc gcgggacagg
     1321 ggacgacggc cctggaaatc ctggagcagg cgcccgatgt ggaggagatc ctcgtcgcca
     1381 tcggcggggg cggcctgatc agcggcatca gcctggcggc caaggcgctt cgcccctcca
     1441 tccgcatcac cggcatcgag cccacgggat cgcccacgct gcatgagtgc ctcaaggccg
     1501 gacaagtggt ggagcttcga gagatcacca ccatcgtccc caccatggca tgcgccaaga
     1561 ccgaacccat caactacgag atcgtccggc agtacgtgga cgagatcgtc ctggtcaccg
     1621 acgacgacat gcgcgaggcg gcgcagtggc tgtggttcga gatggggatc gccacggacc
     1681 tgagtggcgc cgccaccatc gccgccctgc ggagcggcaa ggtccggccg gccaagggcc
     1741 gcaatgtctg cgccctggtc tgcggcgccg gtccggatgg gatgcagtag tggcgggaca
     1801 ttctgcgtta gaagtttcct gcggactttg gtttccgcct cacggacgag gaggcggagg
     1861 tcgcacaatg ttcaagatca caagttgtga tcttgaagcg ggggtggaat atcaagtatc
     1921 tcccctttgc attcaccgaa cacggcgcca tcatggcggc gaacgtactg accagctcgc
     1981 gggctgtggg gatgagcgtc ttcgtggtgc gtgccttcgt ccggctgcgt cagtgaatgg
     2041 ccggtcacgc cgaattctcg cggcgactgg acgagcctgg aaagaaattt gacacccagt
     2101 tcacagtcgt ctttgaccct atccgcgacc tcggagaagc gagccaggtg agtagggggg
     2161 gcgggatgaa atcgtgcacg tgttgcgtcg tcggaccccc gacaccccgg agccaagatg
     2221 aagtggcgta aggatcggga ccagagctgg gtctcgaaag aatctctcca cgtctacggt
     2281 ccgatgctgg tgctgaccct cgtgggattc gtggtcgcgt ttcatttcgt caagccggca
     2341 cctcccacgc atctcgtcat cgccacgggt cggaccgatg gggcgtatta tcagttcgcc
     2401 ctccgctatc gagaactgct cgcacggcgc ggcatccagc tcgaggtccg ggagacgtcc
     2461 ggctccgtgg agaacatcca gttgctcgag gatccgagca agggcgtgga tgtggctttc
     2521 gttcaggggg gtacgggcgg cacggccagg tcaggggaac tggtctcgct ggcgagtctg
     2581 tatttcgaac ccctctgggt cttcacgcgc gccagcctgg gtgccgagga catgagtggg
     2641 ctgcgcgggc ggcgcctggc cgtcggaccc gaggggagcg gaacccgagc catcgccgag
     2701 ctattgctgg gcgccaacgg catcgccacg gattcgacat ttttgctacc gctcgccggc
     2761 ctcgaggccg cccaggccct ccaggcgggc ggcgtggacg ctgcgttctt cgtcgcctca
     2821 ccacagtccg cgatcctgcg agaggccgtt cgcctgcagg gcgtcgcctt gatgagcttc
     2881 cagcgggctg acgcgtacac gcgcctctat ccctttctca ccaagctcgt cctgccgcgg
     2941 gggaccctga atctccccgc cgatcttccg ccgcgggaca cgctgctcct ggcgccggcc
     3001 gccagcctgg tggtccggaa tgattttcat cccgccctcg tcgatctctt gctgggagcg
     3061 gccactgaag tccacggtgg cgccggcatg ttcgagcggg ccctgcagtt tccctcggca
     3121 gagaatctgg agtttccact gagcagcgag gcacgccggt acttccggac cgggccttcg
     3181 tttctggcac ggtatctgcc cttctgggcg gccacgctgg tggaccggat gaaggtcatg
     3241 ttgcttccgc tgatcacgct gctcatcccg ctcttcaaag tggttccacc cacctaccgg
     3301 tggcgcgtcc gggcgaagat ctatcggtgg tatcgcgacc tccacgccgt ggataccgcg
     3361 ctggcagagc ggccgaaagg cggggacctc tcagacctgc tccgggaact ggaccggatt
     3421 gaggcggagg tgctacggat cgtgatcccg ctatcctatt ccgagtcgca ctataatctt
     3481 cgtctccaca tcgagtggct ccgcagcgag gtccgcgccg cgcagtccac gttgaaagag
     3541 ggggcgtgaa agtcaaacga cgcgggaaaa ctgacctgag ttcacaggag gagtgatcat
     3601 gccacccgtt ggtatcgcga tcgtgggctg tggcggaatg atcgccggtg tgcacgcggc
     3661 agcgctgaag gagatccccg acgcacgcct ggtgggggcg tggagccggt cgccggagaa
     3721 gacccagcgc ttctcggatc aacacggggt ccgcgcgtac cgcagccatg aagagctcct
     3781 gggggatccc gcagtccagg cggtcagcat ctgtttgccg tcgggccatc atgccgagta
     3841 tggtgtgaag gccgccgccg ccggaaagca cgtgatcgtc gaaaagccca tcgacatcag
     3901 catccccaag gccatgagtc tgatcgaggt ctgccggaag aacggtcgaa ggctgtccgt
     3961 gatcttccag aaccggtata ccccggcggc caagaaggtt cgcaaagccc tggaccaggg
     4021 gctcctcggg cgattgatcc tgggggacgc gtacatcaag tggtatcgct cgccggagta
     4081 ctacaagagc aacgcctggc gggggacgaa ggccattgac ggcggcgggg cgctcatcaa
     4141 ccaggccatt cacaccatcg atctcttgca gtggatgatg ggcggagtcc agcggctgaa
     4201 tggcctggta cgcacgtcca cccacgccat cgagtccgag gacct
//
LOCUS       DBNGZC010000426         1322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1503868, whole genome shotgun sequence.
ACCESSION   DBNGZC010000426 DBNGZC010000000
VERSION     DBNGZC010000426.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1503868"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="AC16G0_07555"
     CDS             complement(<1..1179)
                     /locus_tag="AC16G0_07555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5099511.1"
                     /translation="MKRILLTNFHPQQDGGGGHMRYVRTILDSPLRREFEFGVAAPQG
                     SAVWSTGRALNAPTFACDFPGNLKEIPQMVRAVRRFEQIYREWTPALIHMNGSRDQGI
                     VVLWKTFCGRAVPCVRAHLAVRCIPNNLYNRWSYLKMVQGHIYIGHSAKNIAWAGGFL
                     KPRNARVIPVGVALDHWKPMQKDPGALARFGLGPTEFVFGSHAGMGQHKRTDLFLQAA
                     ALALRRGARPFKILLRGKEDEVRESQRLASELDLHNLLYASHEPDPRGYLSLIDVGFI
                     LSESIEAVSYAARELMAMGKPLISSNYAGLVENVDDGLNGRLVECGDVESVAEAIGWF
                     LALDAANLERLGQNARRKAEQIFGVDRQVQGLLLFYRVVLERWEKEPRAAGREPRGGS
                     R"
BASE COUNT          230 a          454 c          413 g          225 t
ORIGIN      
        1 gcggctcccg cctctcggct cgcggcccgc ggcccgcggc tctttctccc agcgctccag
       61 caccacgcga taaaacagca ggagcccttg gacctgccgg tccacgccga agatctgctc
      121 ggccttcctt ctggcgtttt gccccagacg ctccagattg gcggcgtcga gggccaagaa
      181 ccagccgatg gcctcggcca cgctttcgac gtccccgcac tcgacaagcc tcccattcag
      241 cccgtcgtcc acgttttcga ccagaccggc gtagttggag ctgatgagcg gcttccccat
      301 cgccatcagc tcccgcgcag cgtaggagac ggcctcgatg gactcgctca ggatgaaccc
      361 cacgtcaatc aaggagaggt acccccgcgg atccggctcg tggctggcgt acaggaggtt
      421 gtgcaggtcc agctcggacg ccaggcgttg actctcccgg acctcgtcct ccttgccgcg
      481 aaggaggatc ttgaaggggc gcgccccgcg ccggagcgcg agggccgccg cctggaggaa
      541 gaggtcggtg cgcttgtgct gacccatgcc ggcatgggag ccgaacacaa actccgtcgg
      601 ccctagaccg aacctggcga gggcgcccgg atctttctgc atcggcttcc agtgatccaa
      661 tgccacaccg accgggatca ctcgggcgtt ccgcggcttc agaaaccccc ccgcccaggc
      721 aatgttcttg gcgctgtgac cgatgtagat gtgcccctgc accatcttca ggtaggacca
      781 gcgattgtag agattgttcg ggatacagcg gacggccagg tgcgcgcgca cgcagggcac
      841 agcccggccg caaaacgtct tccagagcac gacaatgccc tgatcccggc tcccattcat
      901 gtggatgagg gcaggcgtcc attcccggta gatctgctcg aatcgccgca cggcgcggac
      961 catctgcggg atttccttca ggttgccggg gaagtcgcag gcaaacgtgg gagcgttcag
     1021 ggcgcgtcca gtggaccaga ctgccgaccc ttggggcgcg gcgacgccga attcgaattc
     1081 gcgacggagc gggctgtcca ggatcgtccg gacataccgc atgtgacccc cgccgccgtc
     1141 ttgctgggga tgaaagttcg tgagaaggat tcgcttcatc tcgcgtccat catagccgca
     1201 gcccggctga tgtcaactgc cgcgctcgcc gcagcctcag atcacccccg cccgcagcag
     1261 atcgtgcagg tggagaaccc cttcgggccg gcctgccgcg tcgacgatca agagggaggt
     1321 ga
//
LOCUS       DBNGZC010000427         1648 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1307698, whole genome shotgun sequence.
ACCESSION   DBNGZC010000427 DBNGZC010000000
VERSION     DBNGZC010000427.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1307698"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="AC16G0_07560"
     CDS             <1..233
                     /locus_tag="AC16G0_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006186267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR5099512.1"
                     /translation="MPRIDKSYTGDKRSRLVVIPGRIPNLIDPPRGCRFHPRCPIAKE
                     ACSQDLPEPVEVADGHMVSCLEYQGPKTCSGR"
     gene            285..1277
                     /locus_tag="AC16G0_07565"
     CDS             285..1277
                     /locus_tag="AC16G0_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099513.1"
                     /translation="MKTTEDLLRIEKLVKRYPVVRRGIFRTKQVGLINAVDNVSFGIR
                     RGETLGLVGESGCGKTTTARSVLYLTAPTSGSAFFDGTDILDVFRKGNREEVLKIRRQ
                     LQYVFQDPYLSLNPRWSIAETILEPLRVHRHLPKAEWEDRLLELLELVGLESYHAWRY
                     PHEFSGGQRQRVGIARALAGEPKFLICDEPVSSLDVSVRAQILNLLVELQDRLRLTYL
                     YISHDLSSVRFISNRVAVMYLGKIVEIADVDELFTHPLHHYTYALLAAIPVPDPGASV
                     RQSVLKGEVPSAMNPPSGCRFHPRCVAAQPKCSQGEPPLMLLGEGHLVACHYPR"
     gene            1369..>1648
                     /locus_tag="AC16G0_07570"
     CDS             1369..>1648
                     /locus_tag="AC16G0_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020614592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR5099514.1"
                     /translation="MENRRVDKGVGRGSRFEIEVDGRKIIAYEGETIGSALIAAGVHT
                     FRRTPKRDHPRGMYCGIGLCYECTMVVDGVPNTRACQTLATPGCRVETQ"
BASE COUNT          321 a          508 c          543 g          276 t
ORIGIN      
        1 cgatgccacg gatcgacaag agctacaccg gggacaagcg gagccggctc gtcgtcatcc
       61 cgggacgcat ccccaacctg atcgaccccc cccgcggctg ccgctttcac ccgcgctgtc
      121 cgatcgcgaa ggaagcgtgc agccaggacc tcccggagcc cgtggaggtc gccgacgggc
      181 acatggtcag ttgtctggaa taccaggggc ccaagacgtg ttccggaagg tgaccggaca
      241 ctgcctcggg acgaacagcg gactcggcac atgagcactc tgccatgaag acgacagaag
      301 atcttctccg cattgaaaag ctggtcaagc gctatcccgt ggttcgacgc gggatcttcc
      361 gtaccaagca ggtcgggctg atcaacgccg tggataacgt ctccttcggc atccgccggg
      421 gcgagacgct ggggctggta ggggagagcg gatgcggaaa gaccacgacc gcccggagcg
      481 tcctgtatct gaccgctccc acctcgggga gcgcgttctt tgacgggacg gacattctcg
      541 acgtcttccg gaagggcaac cgagaagaag tcctcaagat ccggcgccag ctccagtacg
      601 tgttccagga tccgtacctt tcgctcaacc cgcgctggag catcgccgag acgatcctgg
      661 agccgcttcg ggttcaccgg cacctcccca aggctgagtg ggaagaccgc ctgctggagc
      721 tgctggagct ggtgggactt gagtcctacc acgcctggcg gtatccgcac gagttcagcg
      781 gaggacagcg gcagcgggtg gggatcgcgc gggcgctggc cggggagccg aaattcctga
      841 tttgcgacga gcccgtctcg tccctggatg tctcggtgcg ggcccagatc ctgaacctgc
      901 tcgtcgagct gcaggatcgg ctgagactca cgtatctgta tatctcgcac gacctcagct
      961 cggttcgctt catcagcaac cgggtcgccg tcatgtacct gggaaagatc gtcgagatcg
     1021 cagatgtgga cgagctgttc acccacccgc tgcaccacta cacctatgcc ctgctggcag
     1081 ccatcccggt tccggacccg ggggccagcg tacgccagag cgtgctgaaa ggggaggtgc
     1141 cgagcgccat gaatccaccg tccggatgcc gattccatcc ccgttgtgtg gcggcccagc
     1201 ccaagtgcag ccagggggag ccgcccctca tgctgctggg cgagggccac ctggtcgcat
     1261 gccattaccc gcgctagcaa gacaggcccg cgagttcgta agggtgattc ataacccgat
     1321 ggtgtccgat tcctggtagg ctgttcggaa ggaggaagga cgggaggaat ggaaaatcga
     1381 agagtagaca agggtgtcgg gcggggaagc cgcttcgaga tcgaggtgga tgggaggaag
     1441 atcatcgcct acgaaggcga gaccatcggc tcggcgctga tcgctgcagg ggtgcacacc
     1501 ttccgtcgca caccgaagag ggatcacccg cgaggcatgt attgcggaat cggcctgtgt
     1561 tacgagtgca ccatggtggt cgatggcgtg cccaacacca gagcctgcca gactctggcg
     1621 acccccggat gccgggtgga gacgcaag
//
LOCUS       DBNGZC010000428         3097 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2580599, whole genome shotgun sequence.
ACCESSION   DBNGZC010000428 DBNGZC010000000
VERSION     DBNGZC010000428.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2580599"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1542)
                     /gene="aspS"
                     /locus_tag="AC16G0_07575"
                     /pseudo
     CDS             complement(1..1542)
                     /gene="aspS"
                     /locus_tag="AC16G0_07575"
                     /inference="COORDINATES: protein motif:HMM:NF012379.7"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
     gene            complement(1542..2813)
                     /gene="hisS"
                     /locus_tag="AC16G0_07580"
     CDS             complement(1542..2813)
                     /gene="hisS"
                     /locus_tag="AC16G0_07580"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR5099515.1"
                     /translation="MIKGVRGAPDILPEDAGKWQALEAVARDVLDRYGYAEIRTPVFE
                     HTELFVRGIGEGTDIVEKEMYTFQDRGGESLTLRPEATASLMRAYAEHGLGAKSSLAK
                     LYLIGPMFRHERPQSGRFRQFHQIDVEAVGSPGPAVDAEVVALLHHLLEAWGLSGIPL
                     HINSIGDRNCRPAYREAFVAYLQAHREALCADCHVRLQKNPLRILDCKQPGCQRIAAG
                     APTITGYLCDPCRDHFAAVRGLLDELKIAHSVNERLVRGLDYYTRTTFEFLNPALGAQ
                     NAVAGGGRYDGLVEELGGPPTPAIGFAIGEERILTSLAAQGTPGDTPACTGVYVATAG
                     SVPLGTALDLAQTLRRRGLRATADLESRSLRGQMRQANKERFRYSVILGSDELVRGQV
                     TLKDMLSGEQREVPLAEVVDQLTRLPEREIV"
     gene            complement(2897..>3097)
                     /locus_tag="AC16G0_07585"
     CDS             complement(2897..>3097)
                     /locus_tag="AC16G0_07585"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HIR5099516.1"
                     /translation="RIYRIGDLASGDDIMFAATGVTDGDLLRGVRFFGGGARSHSVVM
                     RRRSGTVRFIEATHRFDRRPVY"
BASE COUNT          521 a         1097 c          969 g          510 t
ORIGIN      
        1 cttcgcctgc tcggcgtcga tgccgagggc ctggaacatc ttctgctgga cctcgcggcg
       61 gtggatccgg atgctcccgc cgccgatctc ggccccgttc agcaccaggt cgtaggcctt
      121 ggcccggatc ttcccggggg cgctctcgaa gagttacaga tcctcgtcca ccggcgaggt
      181 gaaggggtgg tgcatggcgc tccaccgctt ctccttctcg tcccactcga gcagcgggaa
      241 gtcggtgacc caggtgatgg ccagcgcctt ggaatcgatc agcttcagct cctgcccgaa
      301 gcgcaggcgc aacctcccca tggcatccgc agcctgtctg gacctgtcca cgcacaggag
      361 cagcagatct ccctcctctg atcgcatccg ctctcgaaag cgctgcagca cctcctccga
      421 caggaacttc gcaaccgggg actggacacc ctcggacccg atcttgaacc aggcgagacc
      481 cttggccccg aagcccttgg cgaagtcctc gagctgatcg agctggctcc gggaatagcc
      541 gccgcagcct ttggcgttca acgccagggc ccgccccccg gctgccagcg gctgcgcgaa
      601 ggcgcggaag gcgccccccg cgaacaggtc tccaacgtcc tggagttcca gaccgaagcg
      661 caggtccggg gcatccaggc cgaagcgccg aatggcctca tcgtaggtga ggcgcgggaa
      721 cggccggacc agctccacac ccttgacctc gcggaacagc tcgaccacca ggccctccat
      781 ggtgctcaga acgtcctcgc gatccacgaa ggacatctcc aggtcgatct gcgtgaactc
      841 cggctggcgg tcggcccgaa ggtcttcatc ccggaagcac ttcacgatct ggaagtagcg
      901 gtcgaagccc gagaccatca gcaattgctt gaagagctgg ggcgactggg gaagggcata
      961 gaactgccca gggttcaccc ggctcggcac caggtagtcg cgggcccctt cgggcgtgct
     1021 cttggtgagc acgggtgtct ccacctccag gaacccctcg ccgtccaggt agcgtcgggt
     1081 cacctggcag aaccgatgtc gcaggcgcag gttccgctgg aggggcgcac gccgcaggtc
     1141 cagatagcga taggtcagcc ggatctcttc ggccacgtcg gtcgtctcct cgatggggaa
     1201 gaccggcgtc ttggcctcgt tcaggatgac cgcctcctgc gccaccacct ccacctcgcc
     1261 ggtgggaaag tccagattca ccgtccccgc cgggcgcaag cgcacctccc cccggacggc
     1321 caccacatac tccgaccgca tctcctcggc gcactcatgg gcggcggtga aaggttcggg
     1381 gttgaaaacc acctgcacga tcccctgccg atcccgcagg tgcacgaaga tcaggccgcc
     1441 gaggtcgcgc cggcgatcca cccagccgaa tagggtgacg acccggccca cgtggtcggg
     1501 tcggacctcg ccgcagtaga gggttcgctc ccagcccttc attagacaat ctcccgttcg
     1561 ggcaatcgcg tgagctggtc cacgacctcg gccaagggaa cctcccgctg ctcacccgag
     1621 agcatgtctt tcagggtcac ctgcccccgg accagctcgt cgctgccaag gatcaccgag
     1681 tatcggaacc gctccttgtt ggcctgccgc atctgtcccc ggaggcttcg tgactccagg
     1741 tctgcggtcg cccggagtcc ccggcgccgg agagtttgtg ccaggtcgag cgccgtcccc
     1801 agaggaacgg atccagccgt ggccacgtac actcccgtgc aggccggcgt gtctcccggg
     1861 gtcccctgag cggccagaga cgtgaggatc cgctcctccc cgatggcgaa gccgatggcc
     1921 ggcgtgggag gcccccccaa ttcctccacc aggccgtcgt accgtccgcc cccggccacg
     1981 gcgttttgcg cgcccagggc cgggttcagg aactcgaagg tcgtccgcgt gtagtaatcc
     2041 aggccccgca ccagccgctc gttcacgctg tgggcgatct tcagctcatc caggaggccc
     2101 cgaaccgctg cgaaatgatc gcggcagggg tcgcagaggt acccggtgat cgtcggcgcg
     2161 ccggccgcga tccgctggca acccggttgt ttgcaatcca ggatgcgcag gggattcttc
     2221 tggaggcgca catgacagtc ggcgcacagg gcctcccggt gtgcctggag ataggccacg
     2281 aacgcttcgc gatacgccgg gcggcaattg cggtcgccga tgctattgat gtgcaggggg
     2341 attcccgaga gcccccaggc ctccagcagg tgatgcagga gggccaccac ctcggcgtcc
     2401 acggccgggc cgggggaccc gaccgcctcc acatcgatct ggtgaaactg tcggaagcgg
     2461 cccgactgcg gacgctcgtg ccggaacatc gggccgatca gatagagctt ggccaggctg
     2521 ctcttggcac ccaggccgtg ctcggcatag gctcgcatca gcgacgctgt cgcctcgggc
     2581 cgaagggtca ggctttcgcc cccccggtcc tggaaggtgt acatctcctt ctcgacgatg
     2641 tccgtcccct cgccgatccc gcggacgaac agctcggtgt gctcgaagac cggggtccgg
     2701 atttcggcat agccgtaccg gtcgagaacg tcacgcgcga ccgcctccaa ggcttgccat
     2761 ttccccgcat cctcgggaag aatatccggt gccccccgta cgcctttgat cacctgggaa
     2821 cccctcgctg ataaccgatc acagatgttc gattacagtc gtcagacggc cggcccacgt
     2881 ctgcggtctc cgtgtgtcag tagaccggtc ggcggtcgaa ccggtgggtg gcctcgatga
     2941 accgaacggt ccccgagcgg cgccgcatga ccaccgaatg gctccgggcc ccgccgccga
     3001 agaaccgtac accgcgaagc agatccccat cggtgacgcc cgtggccgca aacatgatgt
     3061 cgtctccgga cgccaggtcg ccgattcggt agatccg
//
LOCUS       DBNGZC010000429         1590 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2287848, whole genome shotgun sequence.
ACCESSION   DBNGZC010000429 DBNGZC010000000
VERSION     DBNGZC010000429.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2287848"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1295)
                     /locus_tag="AC16G0_07590"
     CDS             complement(117..1295)
                     /locus_tag="AC16G0_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR5099517.1"
                     /translation="MDGMIYLDNAATSFPKPDEVHDKMISFYRTCGVNPGRSGCDMAL
                     QAEALVDDARRKLSALFNPSPVSAGKSKDPSRLVFTSNATHSLNLILKGTIGPGDHVI
                     TTWMEHNSVLRPVNHLVRLGAKATFLRPGADCRVDPDDIRRAIQPNTKLVIVNHGSNV
                     CGVVQDVPAIGAVCREAGVPLAIDAAQTAGAFPIDMAGWNVSFVAFTGHKSLLGPTGT
                     GGICVADDAEIASTMWGGTGVRSVEPYHLEEYPWRLEAGTLNILGIAGLSAGVDWIND
                     HGLKVIREREDALAAMLAQGLREIRGVTVYFPADGQPRVPTLSVTVKGWDPSDVGTLL
                     DVDYNILTRTGLQCAPKVHEHLGTAPRGTVRFSVGPLNTQEDILRAVKAVGEIAAIRA
                     "
BASE COUNT          282 a          512 c          506 g          290 t
ORIGIN      
        1 caaaagaagt gttacctgta aggcaaggtc cccgtcggca cgtcccttcc ggcaggttcc
       61 acacatgccg atcactggct cttccccgcg gcggtcgcgg acggaggagg tgcggctcag
      121 gccctgatgg ccgcgatctc cccaaccgct ttgacggccc tgaggatgtc ctcctgcgta
      181 ttgagggggc cgaccgagaa gcgcaccgtg ccgcgcggtg ccgtgccgag gtgctcgtgt
      241 accttggggg cgcactgcag gccggtgcgg gtgaggatgt tgtagtccac atccagaagg
      301 gtgccgacgt ccgaggggtc ccaccctttt accgtgacag acagggtcgg cactcgcggc
      361 tgcccatcgg ctggaaaata cactgtcacc ccgcggatct cccgcaaccc ctgcgccagc
      421 atcgcggcca gtgcatcctc ccgctcgcgg atcaccttca ggccgtggtc gttgatccaa
      481 tccacgccgg cagagagccc cgcaatcccg aggatgttga gggtgccggc ctccaagcgc
      541 cagggatatt cctccaggtg atacggctcc accgagcgga cgccagtccc gccccacatg
      601 gtcgaggcaa tttcggcatc gtccgccacg caaatcccgc cggttcccgt ggggcccagc
      661 aggctcttgt gtccggtgaa agccacgaag ctgacgttcc agcccgccat gtcaatcggg
      721 aaggcgccgg cggtctgggc tgcgtcgatg gccaggggga ctcccgcctc gcggcagacg
      781 gccccgatgg cggggacatc ctggactacc ccgcagacgt tcgagccgtg gttgacgata
      841 accaacttgg tgttgggctg aatggcgcgc cggatgtcgt ccggatccac ccggcaatcc
      901 gcaccgggtc gaaggaaggt ggccttggcg cccagcctga ccagatggtt gaccggccgg
      961 aggaccgagt tatgttccat ccacgtcgtg atgacgtgat cccccggacc aatcgtccct
     1021 ttcaggatca ggttgaggct gtgggtggcg ttcgaagtga agaccagccg ggagggatct
     1081 ttcgacttgc cggccgacac cgggctgggg ttgaacaatg cgctgagctt acgccgggcg
     1141 tcatccacca aggcctctgc ctgcagggcc atatcgcacc ccgagcggcc tgggttgacc
     1201 ccgcaggtcc gatagaagct gatcatcttg tcgtggacct cgtcaggctt cggaaatgac
     1261 gtcgccgcgt tgtccaggta aatcatgccg tccatggagg ccctcctgca gagtacgctt
     1321 cggattccga tcctgcgatc attcaggcgt cgaagaaaat ctacaccgaa ctgcctcgaa
     1381 ctgcgggcaa gcgggatggg tttgacggcg tatgttggac gggaccgaga atctttgtca
     1441 agggcgtaga tgcagaggtg cggagagccg ccctttggat gtgctcgatc agggcgccct
     1501 ggcatgcttg tcgccgcccc caaatcgcac agcctcaccc gaagccaatc cacccaaggt
     1561 ttcgtcttga caaccccttc gggtgcacgg
//
LOCUS       DBNGZC010000430         2168 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1897213, whole genome shotgun sequence.
ACCESSION   DBNGZC010000430 DBNGZC010000000
VERSION     DBNGZC010000430.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1897213"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="AC16G0_07595"
     CDS             complement(<1..301)
                     /locus_tag="AC16G0_07595"
                     /inference="COORDINATES: protein motif:HMM:NF040248.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099518.1"
                     /translation="MAKYIAGRLAAMLPVLAVVAVTVFLLIHLIPGDPASVMLGPDAS
                     VVQVNQLRHELGLDRPLWVQFVLWLGHLLQGDLGESIFLRRKVGQAIWEHLGPTAS"
     gene            complement(379..1953)
                     /locus_tag="AC16G0_07600"
     CDS             complement(379..1953)
                     /locus_tag="AC16G0_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099519.1"
                     /translation="MRLLHFNSRLAASVLALLLVLVVPGGPARGAEPVQGGVIRVATN
                     AEPPTLDWQSSTTTATRLVAWHIFESLFALDQDYEVKPLLAQGFSVSPDGLRYTIRLR
                     TGIKFHNGQPMRPEDVIASLERWGKLSSGGRETFKFIKQIRRVDDATIEIGLARAFTP
                     LITNLGDPKQAAVIMPKSVAESSGLKPATDLIGTGPYSFKRWTPGQEIVLVKNPAYAS
                     RTENWGGITGRKVAYADEIDFFPVTDPQVRLAGVSTGQYDLALELPQDVYAGVRANPN
                     LTPDMVKVFNWMGAVFNKANGVFTDERLRQAALYAMNPADLMAAAAGPKEFWKLDPGL
                     FFPEQKGLYSTAGSDIYNHQNLDKARALMKAAGYHGQKIVVMSTKDYTWNNNAAQVLV
                     PELQAVGFNVDSQVFDWPTLINRRTKKDLWDIFLTGFSPSIDPTAVIFFGSNWPGWYE
                     SKALDAQLSRWSQTPVTDAAARKALMDQIQTTFYKEVPVAKFGNSYGLEVYNNQQLHG
                     YTSLFDVRFWNTWVTR"
BASE COUNT          374 a          699 c          717 g          378 t
ORIGIN      
        1 gactcgccgt cggacccaga tgttcccaga tcgcctggcc caccttccgc cgcaggaaga
       61 tcgactcgcc caggtcaccc tggagcagat gccccagcca cagcacgaac tgcacccaca
      121 ggggtcggtc gaggcccaac tcgtgccgga gttggttgac ctggacgacc gaggcgtccg
      181 gccccagcat aaccgaggcc ggatcccccg ggatcaggtg gatcagcaag aagacggtga
      241 ccgcgacgac cgccaggacc ggcagcatgg ccgcgaggcg cccggcaatg tactttgcca
      301 cttgcctccc cccgggcgcc cgcctcgctc accccgcgcc accgaaaacc cgacctccgc
      361 ccgcggcgcc tccgacgctc agcgggtcac ccacgtgttc cagaaccgca cgtcgaataa
      421 gctcgtatag ccgtgcaatt gctgattgtt gtacacctcg aggccgtaag agttcccgaa
      481 cttggcgacc ggcacttcct tgtagaaggt ggtctggatc tggtccatga gggccttgcg
      541 cgcggcggcg tcggtcaccg gtgtctggct ccaccggctc aactgggcat ccagggcctt
      601 gctctcgtac catcccggcc agttgctgcc gaagaagatg acggccgtgg gatcgatcga
      661 gggcgagaag cccgtcagga agatgtccca caggtctttc ttcgtgcggc ggttgatgag
      721 cgtcggccag tcgaacacct gagagtccac gttgaatccc acggcctgga gttccgggac
      781 gagcacctgc gccgcgttgt tgttccaggt gtagtccttg gtggacatca ccacgatctt
      841 ctggccgtga tagcccgccg ccttcatgag ggcccgggcc ttgtccaggt tctggtgatt
      901 gtagatatcc gacccggcgg tgctgtacag gcccttctgc tcggggaaga acaggccggg
      961 gtcgagcttc cagaactcct tcggccccgc cgcggccgcc atcaggtccg ccgggttcat
     1021 cgcatagagc gccgcctgcc gcaggcgctc gtcggtgaat acaccgttgg ccttgttgaa
     1081 gaccgctccc atccagttga acaccttgac catgtcgggg gtcaggttgg ggttcgccct
     1141 cacccccgcg tagacgtcct ggggaagctc cagggccagg tcatactggc cggtggacac
     1201 cccggcgagc cgcacctggg ggtcggtgac ggggaagaag tctatctcgt cggcgtaggc
     1261 caccttccgg cccgtgatcc cgccccagtt ctcggtgcgt gacgcgtaag ccgggttctt
     1321 gaccagcacg atctcctgac cgggcgtcca ccgcttgaag ctgtacggac ccgtgccgat
     1381 caagtcggtc gcgggcttga ggccggagga ctcggcgacg ctcttgggca tgatcaccgc
     1441 cgcctgcttg gggtcgccca ggttggtgat gagcggtgtg aaggcccggg ccagaccgat
     1501 ctcgatcgtg gcgtcgtcga ccctcctgat ctgcttgatg aacttgaacg tctcgcgacc
     1561 gccgctgctg agtttgcccc agcgttccag ggaggcaatg acgtcctccg ggcgcatggg
     1621 ctgaccgttg tggaacttga tgccggtccg aagacggatg gtgtagcgca gcccgtcggg
     1681 gctgacgctg aagccctgag ccaggagcgg tttgacttca tagtcctggt ccagcgcgaa
     1741 caggctctcg aagatgtgcc aggcgacgag ccgcgttgcc gtcgtcgtgg acgactgcca
     1801 gtcgagggtg gggggttcgg cgttcgtcgc tactcggatc accccgccct gaacgggctc
     1861 ggccccgcgg gccggcccgc caggaacgac gaggaccaac aggagcgcca ggacagaagc
     1921 ggcgagccgg gaattgaaat gaagcaggcg catgggagtc ccctcctccg gaaacggatc
     1981 tggatgcgaa agccttatcg agagagtgcg gctcttgagg cagcggggcc cgatcggcgg
     2041 cacaaagtca gcgcaggccc gcccatgccg aggcgatgag actcatggtg gagtgggagc
     2101 gcccggtggt caagtgaaaa ctggtccgaa aagctgggca ggtggccaca ctggaggtgc
     2161 ttgcacac
//
LOCUS       DBNGZC010000431         1963 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1699024, whole genome shotgun sequence.
ACCESSION   DBNGZC010000431 DBNGZC010000000
VERSION     DBNGZC010000431.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1699024"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="AC16G0_07605"
     CDS             complement(<1..546)
                     /locus_tag="AC16G0_07605"
                     /inference="COORDINATES: protein motif:HMM:NF018004.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HIR5099520.1"
                     /translation="MAEIIPFRALRYNPQFVPDLKLVVAPPYDVISPEAQERYHARHP
                     HNVVRLILAKHGSAGTPGPDRYARSARTFAEWRAGGILRRDPRPAIYLYEQEFSVGDG
                     HRIQRRGFMAMVRLQDYDAKVIFPHERTFSQYKDDRLQLMRACPANLEAILGFYPGPG
                     GAISAALARHMEEEPSVGLTDE"
     gene            890..>1963
                     /locus_tag="AC16G0_07610"
     CDS             890..>1963
                     /locus_tag="AC16G0_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR5099521.1"
                     /translation="MQITDVRSLLIRIPLQRPIRIATREVHAREFVLTFVKAEDGSQG
                     IGYTYAGTVGGSMVRTAIDDVLKPLLLGGDADLIERHWAAMFQHGLLVGRRGALLRAI
                     SAVDIALWDLAAKRAGTPLFRLLGGFQGEVPAYASGGYYMEGKGTAGLAEEMQRYLRA
                     GFNAVKIKVGGVSIAEDATRVKAAREAIGSGIRLALDANNAYKTVAEAIRAARTFEPY
                     DIWWFEEPLPPDNIPGHAELAAAVNIPVATGEIHSSRWDFRDIIAIKAAQILQPDAGV
                     LGGISEWMKVAHTAASFDLPVAPHWHADLHIHLVGAVSNALTVEYFFLEEDIYNFERI
                     LLERLVPSRGHLHIPERPGLGLVL"
BASE COUNT          339 a          651 c          649 g          324 t
ORIGIN      
        1 ctcgtcggtg agcccgacag acggctcctc ctccatgtgt cgcgccagcg cggcagaaat
       61 agccccgccc ggtcccggat agaatcccag gatggcctcc aggttggccg ggcaggcacg
      121 catgagctga agccgatcgt ccttgtactg ggagaaggtg cgctcgtggg ggaaaatgac
      181 cttggcgtca taatcctgga gccgtaccat cgccatgaag ccgcgccgtt ggatgcggtg
      241 cccatcgcca accgagaact cctgctcgta gagatagatg gccggccggg ggtcgcgacg
      301 gaggatcccg ccggctcgcc attcggcgaa ggttcgagct gatcgggcgt agcggtccgg
      361 ccctggcgtc cccgcgctgc cgtgtttggc cagaatcagt cgcaccacgt tgtgcgggtg
      421 gcgcgcatga tatcgctcct gcgcctccgg ggagatcacg tcgtagggtg gcgccaccac
      481 cagcttcaaa tcggggacga attgaggatt gtaccggagg gcacgaaagg gaatgatctc
      541 ggccatgcgc ttgccctggg gaacaaaaaa ggtcacgcgt tgggcggacc ctcgaaccgt
      601 gacccgcggc gaccggccga cggggggccg ccagccggtc ccggcgcgga agcgaaacta
      661 cttgggctgc cctggcgtgt caagggaaaa ggcccagagg ggatcgtcct ctgcctcctg
      721 cacttcccgc ccccgcggcc ggcttcaagt ctctccgttt cgtcgtgatc ctccgggatc
      781 ctccggggga gccccgtctc aacttcgggc tccggcgagc cggggcttag ccgttcacgg
      841 ggtcggtgcc gtccgacacc cactcaccct gcgtgcaatg gaggcaccta tgcagatcac
      901 tgatgttcga agcctactca tccggatccc cttgcagcgt ccgatccgca tcgccacgcg
      961 ggaagtccac gcgcgggagt tcgtcctcac cttcgtgaag gcggaggacg gaagccaggg
     1021 gatcggttac acgtacgccg gcacggtggg cgggagcatg gtccggaccg cgatcgacga
     1081 cgtattgaag ccgctcctgc tgggcggcga cgcagacctg atcgagcggc actgggccgc
     1141 catgttccag cacgggctgc tcgttggccg gcgaggagcc ctgctacgcg ccatctcggc
     1201 tgtcgacatc gccctgtggg atctggcagc caaacgggcc ggcacccccc tcttccggct
     1261 gctcggcggg ttccagggag aggtgcctgc ctacgcctcg ggcggctact acatggaagg
     1321 gaagggaacg gccggcctgg cggaggagat gcaacggtac ctccgcgccg gcttcaacgc
     1381 cgtgaagatc aaggtggggg gcgtctccat cgcggaagac gcgacccggg taaaggcggc
     1441 gcgagaagcc attggctcgg ggattcgtct ggccctggac gccaacaatg cctacaagac
     1501 cgttgccgag gccatccggg ccgcaaggac cttcgagccg tacgacatct ggtggtttga
     1561 ggagcccctg cccccggaca acatcccggg gcacgccgaa ttggccgcgg ccgtgaacat
     1621 ccctgtggcc acgggcgaga tccactccag ccgatgggac ttccgggaca tcatcgcaat
     1681 caaggccgca cagatcctcc agccggacgc gggcgttctg ggcggcatca gcgaatggat
     1741 gaaggttgcc cacaccgccg ccagcttcga cctgccggtg gcaccccact ggcacgccga
     1801 tctgcacatt cacctggtcg gcgccgtctc caatgccctg acggtggagt acttcttcct
     1861 agaagaggat atctacaact tcgagcggat cctcctggag cggctcgtac catcccgcgg
     1921 ccaccttcat atccccgagc ggcccggact gggcctcgtc ctg
//
LOCUS       DBNGZC010000432         6171 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_231954, whole genome shotgun sequence.
ACCESSION   DBNGZC010000432 DBNGZC010000000
VERSION     DBNGZC010000432.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6171)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6171)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6171
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_231954"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="AC16G0_07615"
     CDS             <1..646
                     /locus_tag="AC16G0_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR5099522.1"
                     /translation="REYEVERHQNVLLMLDAGRMMTAAVGSLTKLDCAVNAALLVTHA
                     AVGHGDKVGLLVFAEDILAYLPPRTGRTQIGRVLEALYQIQPSLVEPDYGAAFRYLAT
                     RRLQRALVLVFTDLVDARASGRLLRHVAALMPHHLALLAAIADPTLQRYAREAPASAT
                     AVYRQAVARDLLEERAEALNGITARGGLALDVAPEGLNLAVVNRYLEVKRRGLL"
     gene            complement(690..1694)
                     /locus_tag="AC16G0_07620"
     CDS             complement(690..1694)
                     /locus_tag="AC16G0_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA];
                     GO_process: GO:1901893 - positive regulation of cell
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HIR5099523.1"
                     /translation="MDLTRFVEERRPDWERLDHLAKQAGQTLGRLGKVDLRELGALYR
                     QASSDLARVQAVRADPELVEYLNNLVVRAHGIIYQPEPARLKALTTFFAREFPTLVRQ
                     EWRPILLALFVDVLPALWCLLMAALDPSFIDAVAPPGLRERLDNGELWVYRINPIQPL
                     ASSAILTNNITVTFTFFALGIVFGVGTLWGLAYNGIHFGSIAVLVNQTRMAREFWAFV
                     LPHGVLEIPAIYIGGGAGFILGAALLFPGDLSRKDALVVRGRVAVKLVLGCVPLLIIA
                     GIIEAFFSPLPPSSMPIGAKFLVGAALFSLLLLYLLLGGRTPVKSITKEPRQEGSTRS
                     "
     gene            1766..2605
                     /locus_tag="AC16G0_07625"
     CDS             1766..2605
                     /locus_tag="AC16G0_07625"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR5099524.1"
                     /translation="MEPHGHEREKGASGDERMAVRTPEHVELHFLLAGPGNRFLALMV
                     DLVIQWLLLLGILLALGGFLWMTKWSFGEMISKQMGTRLALWATAGLILVFFLLQWGY
                     FTFFETIWSGQTPGKRRQRIRVIRENGRPIGFAEAAIRNLLRGVLDSQPFPLHAIGLV
                     VAVLNSRFKRLGDFAAGTVVIVERRQSVPKAGPRLRPAAHAVLPKTPLRVRQLTPEEV
                     ATLQAYLRRRDELSAEVRLTMAHKIAVSLMQRLDISQPMDMTYDAFLQWLDEEVRKSQ
                     AFR"
     gene            2974..3732
                     /locus_tag="AC16G0_07630"
     CDS             2974..3732
                     /locus_tag="AC16G0_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020590110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR5099525.1"
                     /translation="MKAHRVVKALIGLAGLCLMAVQPNVAAAGAARTVTVATDATWPP
                     MEMVDATKQIVGFDIDYLKAVAKEAGFGVEFKNTAWDGIFAGLAAGRYDVIASSVTIT
                     DERRKQYDFSDPYINVGQILVVPKTEKGKALADLKGKKVGAQIGTTGAFEIKKVAGVE
                     LKSYDEIGLAFEDMAAGRISGVVCDEPTAAHYALQRKEYKEKFKIVGEPFTQESYGFV
                     VQKGKKDLVDLLNKGIKAVKAKGIDEQFRKKWLR"
     gene            3752..4549
                     /locus_tag="AC16G0_07635"
     CDS             3752..4549
                     /locus_tag="AC16G0_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015773648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR5099526.1"
                     /translation="MEDKTQPPAVVEVGDGAAIPLKSDVGLFTAWRLAFFGAIATIAC
                     LAVTRPDPYFRILTFVPDGVLVTFKVTVGAILLALVLGLFTGLGRLAKNRFINGAASL
                     YVEVIRGIPLLVQIFYIYYALGRIVHIPDIYSAVIAMAVCYGAYMGEIFRAGIQSIPR
                     GQMEAARSLGMTHARAMRHIIIPQAFKVVLPPIGNEFIALLKDSSLVSILAVSDLLRR
                     GREFASESFSYFETYTIIALIYLVLTLFFSKLIGFMEERIGVGKQPS"
     gene            4551..5273
                     /locus_tag="AC16G0_07640"
     CDS             4551..5273
                     /locus_tag="AC16G0_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013514885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR5099527.1"
                     /translation="MVEAREISKYFGRFKALDRVSFDVYDGEKVVVIGPSGSGKSTLL
                     RSINGLETVDRGRILVDGIDINDPKTDINKVREEVGMVFQSFNLFPHKTVLENLTLAQ
                     IIVRKRPKAEAAERAMGLLKKVGIPEKANAYPSQLSGGQQQRVAIARSLAMGPKLMLF
                     DEPTSALDPEMIGEVLDVMKSLAREGMTMVVVTHEMGFAREVSDRVVFMDSGAIVEEA
                     PPAHFFTNPGQERTKLFLSQIL"
     gene            complement(5361..>6171)
                     /locus_tag="AC16G0_07645"
     CDS             complement(5361..>6171)
                     /locus_tag="AC16G0_07645"
                     /inference="COORDINATES: protein motif:HMM:NF012876.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HIR5099528.1"
                     /translation="FQKAMQGVAYTSEPSVSIITNKPTIFYSAPIQDPSGQVLAVVRI
                     RASLDDFAQVVDGDAGSHGPGSYGMLLDEYGIRLAISTTPTNRAEVTSKFLYRAIGPV
                     PPEAEKAMVAEKRFGRAAESTVGVLSIPALVPTLQSPTDLPILGMNADLNTEQNIGYA
                     VRMRNKPWRYVLVTPQSTFYAETYGLQMTVLVVGLVSAILAAGIGIFVGRTISNPLLQ
                     LARVADRMSLGDLDAKIELGRRDEIGLLGESLSRMQASLRAAMERLRAKRT"
BASE COUNT         1142 a         1877 c         2043 g         1109 t
ORIGIN      
        1 ccgggagtat gaggtggaac ggcaccagaa cgtcctcctc atgctggacg ccggccggat
       61 gatgacggcg gcggtgggca gcctgacgaa actggactgc gcggtgaatg ccgccctgct
      121 cgtgacccac gcggccgtgg ggcacgggga caaggtgggt ctcctggtct tcgccgagga
      181 tatcctggcc tacctgcctc cgcggaccgg caggacgcag atcggacggg tgctggaggc
      241 gctgtaccag atccagccga gcctggtcga gcctgactac ggcgccgcct ttcggtatct
      301 ggcgacccgg cgcctccagc gggccctcgt cctggtcttc acggatctgg tggatgcccg
      361 cgcctcgggc cgcctgctgc ggcacgtggc ggcgctcatg ccgcaccacc tggcactcct
      421 tgccgccatc gcggatccca cgctgcagcg gtacgcgcga gaagcccccg cgtcggccac
      481 ggccgtgtac cggcaggcgg tggcgcggga cctgttggag gagcgcgccg aggcgctcaa
      541 cggcatcacg gctcggggcg gtctggccct ggacgtggcg ccggaagggc tgaacctggc
      601 cgtggtgaac cgatacctgg aggtcaagcg gcgaggactg ctctaaagga atggccggta
      661 accacaaacg acgaagtctc cgtgaggtct caagaacgcg ttgatccttc ttgccttggt
      721 tccttggtta tggatttgac cggcgttctt cctcccagca ggaggtagag cagcagtagc
      781 gagaagaggg cggcgcccac cagaaacttg gcgccgatgg gcatgctgct ggggggcaag
      841 ggtgagaaga aggcctcgat gattccggcg atgatcagga ggggaacgca acccaggaca
      901 agcttcacgg ccactcggcc gcgcacgacc agggcgtcct tccggctaag gtcgccgggg
      961 aagagcaacg cggcgcccag aatgaatccg gctccgcccc caatgtagat ggcggggatc
     1021 tccaggacgc cgtgggggag cacgaaggcc cagaactcgc gcgccatccg ggtctggttg
     1081 acgaggactg cgatcgagcc gaagtggatc ccgttgtagg cgaggcccca caaggtgccg
     1141 acaccgaaga cgatcccaag ggcgaagaaa gtgaaggtga cggtaatgtt gttggtcagg
     1201 atggcgctgg atgcgagggg ttggattgga ttgatgcggt agacccataa ctcgccgttg
     1261 tccagacgct cccgcagacc gggtggagcc acggcatcga tgaagctcgg atcgagagca
     1321 gccatcagga ggcaccagag ggcggggagc acgtccacga acagcgccag gaggatgggt
     1381 cgccactcct gccggacgag cgtcgggaac tcgcgcgcga agaaagtcgt cagggccttc
     1441 aggcgggcgg gctcgggctg gtagatgatc ccgtgggcgc ggactaccaa attgttgagg
     1501 tactccacaa gctcaggatc ggcccggact gcctggaccc gagccagatc ggatgacgcc
     1561 tggcgataga gggcgcccag ttcccgcaga tctactttcc ccaggcggcc cagggtctgg
     1621 ccggcctgct tcgcgaggtg gtcgagccgt tcccaatcgg gccggcgttc ttcaacgaag
     1681 cgggtcagat ccatggctat cccggctcaa cgggcggagg gggttcaagg cgattcgctc
     1741 gcttggtatg caaggggctg aacccatgga accgcatgga cacgagcggg agaagggtgc
     1801 gagtggcgac gagcggatgg cggtccgcac gccggagcac gtggaactgc atttcctctt
     1861 agctggtccc ggaaaccggt tcctggccct gatggtcgac ctggtcatcc agtggctgct
     1921 gctcctgggc atcctgctgg ctctgggtgg atttctgtgg atgaccaaat ggtctttcgg
     1981 cgaaatgatc agcaagcaga tggggactcg tctcgccctc tgggccactg caggtctcat
     2041 tctggtcttt tttcttctgc agtggggata cttcaccttt ttcgagacga tctggtccgg
     2101 ccagactccc ggaaagcgcc gccagaggat ccgggtcatc cgagagaacg gccggcccat
     2161 tggcttcgcc gaagcggcca tccggaatct gctgcgcggc gtgctcgatt cgcagccctt
     2221 ccccctgcac gccatcggcc tggtagtggc agtgctgaac tcccgcttca agcgccttgg
     2281 ggatttcgct gccggcacgg tggtcatcgt ggaacgacgg cagtcggtgc cgaaggcggg
     2341 gccgcgtctg cgccccgcag cccacgccgt cttgcccaag acgccactac gggtccggca
     2401 gctcaccccg gaggaggtgg ccaccctcca ggcatacctg cgccgccggg acgagctgag
     2461 cgccgaagtg cgcttgacca tggcgcataa gattgcggtc tcgctcatgc agcgcctgga
     2521 catctcgcag cccatggaca tgacctacga cgctttcctc cagtggctgg atgaggaggt
     2581 gcggaagtcc caggctttcc ggtagatcga gccaacggcc ggatcctgcg gccctggtcg
     2641 ccgcgtttgc ccttggctct gccgacgccg gtcgtcctcg gagacttctg atgctgcccg
     2701 cccccgaggc ccctccaggt atagccgaag gggaaggcag gcgcaatcga attgcgccca
     2761 cccccggacg cctgtcctct cagccttgac ttcggattcc gattagaggt agcgtctcgc
     2821 cgggttgtag ggtggaattg gatttgcgcc tcctgtcatc ggcgtctcgg cggccgaggg
     2881 ttcgttccgg atcaaggctg tcgggctggc cctgatcagc ttcatattcg caagagcatg
     2941 tggtgtgaaa gcgcataccc aaaggaggag agcatgaaag cacaccgcgt cgtcaaggcc
     3001 ttgatcggtc tcgccggact ctgtctcatg gcagtccagc ccaacgtcgc ggccgccggg
     3061 gccgcacgca cagtcacggt tgccaccgat gccacctggc cgcccatgga gatggtggat
     3121 gccaccaagc agattgttgg ctttgacatc gattacctca aagccgtggc gaaggaggca
     3181 ggcttcggcg tggagttcaa gaacaccgcc tgggacggta tcttcgccgg gctggctgcc
     3241 ggccgatacg atgtcattgc ctcgtcggtg accatcaccg acgagcgaag gaagcaatat
     3301 gatttttccg acccgtatat caacgtcggc cagatcctcg tggtcccgaa gacggagaaa
     3361 gggaaggccc tggcggacct caagggaaag aaggtgggcg cccaaatcgg cactaccggg
     3421 gcattcgaga ttaagaaagt tgcaggggtg gagctgaagt cctacgacga gatcgggctg
     3481 gccttcgaag acatggcggc gggccggata tcgggcgtgg tctgcgatga gccaaccgcg
     3541 gctcactacg ccctgcagcg gaaggagtac aaggagaagt tcaagatcgt aggcgagccg
     3601 ttcacccaag aatcctacgg cttcgtcgtc cagaaaggga agaaggacct cgtcgacctt
     3661 ctgaacaagg ggatcaaggc cgtcaaggcc aaggggatcg acgagcagtt ccggaagaag
     3721 tggctccgat agcgcctccc ccggagagat gatggaagac aagacgcagc ctcccgccgt
     3781 cgtcgaggtg ggcgacgggg ccgcgatccc gctgaagagc gacgtaggat tgttcaccgc
     3841 ttggcgactc gccttcttcg gggccattgc caccatcgcc tgcctcgccg tcactcgtcc
     3901 cgatccgtac ttccgaatcc tgacgttcgt tcccgacggg gtcctcgtca ccttcaaggt
     3961 caccgtcggg gccatcctgc tggccctggt cctcggcctc ttcacgggcc tcggacggct
     4021 ggcgaagaac aggttcatca acggggccgc ctcgctgtac gtggaggtga tccgcggcat
     4081 tcccctgctg gtgcagatct tctacatcta ctacgccctg ggccgcatcg tacacatccc
     4141 cgacatctac agcgccgtga ttgccatggc ggtctgctac ggggcctaca tgggggaaat
     4201 cttccgcgcg ggcatccagt ccatcccacg agggcagatg gaggcggccc gctcgctggg
     4261 catgacccat gcccgggcca tgcggcacat catcatccct caagcgttca aggtcgtgct
     4321 cccccccatc ggcaatgagt tcatcgccct gctcaaggat tcctccctcg tgtcgatcct
     4381 ggcggtgtcg gatctcttgc ggcgggggcg cgagttcgcc tcggagtcgt tcagctactt
     4441 cgagacctac acgatcatcg ccctgatcta cctggtcctc acgctgtttt tttcgaagct
     4501 gatcggattc atggaggagc ggatcggtgt cggcaaacaa ccgtcgtaaa atggtcgagg
     4561 cccgggagat cagcaagtac ttcggccggt tcaaggccct ggaccgggtg tccttcgatg
     4621 tctacgacgg ggaaaaggtg gttgtcatcg ggccgagcgg ctcggggaag agcacgcttc
     4681 tccggtccat caatgggctg gagacggttg atcgcgggcg gatcctggtg gacgggatcg
     4741 acatcaacga cccgaagacc gacatcaaca aagtccggga agaagttggc atggtcttcc
     4801 aatccttcaa tctcttcccc cacaaaaccg tgctggagaa cctgaccctg gcccagatca
     4861 tcgtccggaa gcgccccaag gcggaggccg cggagcgggc catgggactc ctcaagaagg
     4921 tggggattcc cgaaaaggcc aatgcctatc cgagccaact ctcgggaggg cagcagcagc
     4981 gcgtggccat cgcccgctcg ctggccatgg ggccgaaact gatgctgttc gacgagccca
     5041 cgtccgccct cgacccggag atgatcgggg aggtcctgga cgtcatgaag agcctcgcca
     5101 gagaagggat gaccatggta gtggtcaccc acgagatggg cttcgctcgg gaggtgtcgg
     5161 accgagtggt cttcatggat tccggggcca tcgtggagga ggcgcccccg gcgcatttct
     5221 tcacgaatcc cggccaggag cgtacgaagc tgttcctcag ccaaatcctc tgatcgccga
     5281 gggcgaccgc cgccatcttt ccccgccctg ttgcttcgat caagaaggag ggggtgcctg
     5341 ggccgtggga gaagcccact tcatgtgcgc ttcgctcgca ggcgctccat ggcggcacgc
     5401 aagctggctt gcatccgcga gagggattct ccgaggagcc cgatctcgtc cctgcgaccg
     5461 agttcgatct tggcgtccag atcacccagg ctcatccggt ccgccacccg ggcgagttgg
     5521 agcagcggat tcgagatggt gcgcccgaca aagatgccga tacccgcggc gagaatcgcc
     5581 gatacgaggc cgacgacgag gacggtcatc tgcaggccgt aggtctccgc atagaatgtt
     5641 gactgcggcg tgacgagaac gtaccgccag ggcttgttgc gcatgcggac ggcgtagccg
     5701 atgttttgct cagtgttcag gtcggcattc atgccgagaa tcggcaggtc cgtggggctc
     5761 tggagcgtgg gcacaagggc gggaatcgac agcacgccga cagtcgactc ggcggcccgt
     5821 ccaaagcgct tctctgccac catggccttc tccgcttcag gcggtacagg tccgatggca
     5881 cggtacagaa acttgctggt gacctctgct cggttcgtgg gggtcgtgct gatggcaagg
     5941 cgaatgccgt attcatccaa gagcattccg tagctgccgg ggccatgcga tcccgcatcc
     6001 ccatcgacga cctgggcgaa atcatccagg gaggctcgga tgcgcaccac agccaggacc
     6061 tggccgcttg ggtcttggat aggggcagag taaaagatcg tgggtttgtt ggtgatgatg
     6121 gaaaccgaag gttcggaggt gtacgccacc ccctgcatgg ccttctggaa g
//
LOCUS       DBNGZC010000433         2431 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2677486, whole genome shotgun sequence.
ACCESSION   DBNGZC010000433 DBNGZC010000000
VERSION     DBNGZC010000433.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2677486"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1395
                     /locus_tag="AC16G0_07650"
     CDS             187..1395
                     /locus_tag="AC16G0_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002756392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HIR5099529.1"
                     /translation="MELLPEHTIKTLREAARLLTGPKRRRFQAQVTRDYLNGHARQAE
                     RVFGWGRQTVQLGLHELRTGITCLDRYTDRGRHATEADHPQLAHDIRELVEPHAQADP
                     TSPSPFAFTRLTAKAVRRALIERKGYQDEDLPCERTLRNLLNRLGYRLRHVQKTRPLK
                     KIKETEAIFTQVEAAHERAAALEDTLRISIDTKAKVQVGEFSRGGAARGAGPVEALDH
                     DMKPVATLVPFGILEVVSGLLTILFGTTCLTSDFVVDCLEYWWQERRKSLSQVKKLVI
                     DLDNGPETSSRRTQFVRRLVEFADRFSLVIELVYYPPYHSKYNAIERCWGILEKHWNG
                     ALLSHVETVLCWAGTMTWKGQPPEVRLVEKEYERGRGVDKKEMKRYEARLQRSEALPK
                     WSVIITPQAC"
     gene            complement(1392..2030)
                     /locus_tag="AC16G0_07655"
     CDS             complement(1392..2030)
                     /locus_tag="AC16G0_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024751.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR5099530.1"
                     /translation="MAGEGLAPHQKKARRRGANLLLMDESGLLMAPLVRRSWAPRGHP
                     PVIQQKAGHREKVSVAAALWLPPLRDRLGLAYQTLVNGYFSNMEVAEFLGCAVEGLPG
                     PVIAIWDQGTMHKGDPIRELVDQSRGRLDIEPLPAHAPELMPVEQLWTWLKYDRLCNF
                     APEGAHQLNAVVVGELEAIRDDQTRLRNFFHAFDLPLPRALLRQSERDKLPH"
     gene            complement(2139..2402)
                     /locus_tag="AC16G0_07660"
                     /pseudo
     CDS             complement(2139..2402)
                     /locus_tag="AC16G0_07660"
                     /inference="COORDINATES: protein motif:HMM:NF024954.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          462 a          762 c          768 g          439 t
ORIGIN      
        1 atcgacgcgg tcggagacgg cgaacacgtc attgaggcga tggctcacca gaaccacgat
       61 tcagttttcc gatctccgcc tcgagataag gcgataggaa agaaatagcc taccactcct
      121 ctccggccag ccgtatccta tctcctctat cattcttcca gtgtccttga ccagcctcgg
      181 tgacccgtgg aactgctccc ggaacacacg atcaagaccc ttcgcgaggc cgccagactt
      241 ctgaccggtc ccaagcgacg acgcttccag gcccaagtca cccgcgatta cctcaacggc
      301 cacgcgcgtc aagccgaacg cgtctttggc tgggggcgcc agaccgtcca actcggtctc
      361 cacgaactgc gcaccggaat cacctgcctc gatcggtaca ccgatcgcgg acgacacgcc
      421 accgaagccg accaccctca gttggctcac gacatccgcg aactggtcga accccacgcc
      481 caggccgatc ctacgtcccc atccccgttc gccttcacac gtctcacggc caaggccgtc
      541 cgccgtgccc tgatcgagcg caagggttat caggacgagg acctgccctg tgaacgcacc
      601 ctccgcaatc tcctcaaccg tctgggctac cgcctgcgtc atgtgcagaa gacccggccc
      661 ctcaagaaga tcaaggagac cgaggcgatc ttcacccagg tcgaagcggc ccatgaacgg
      721 gcggcggctt tggaggacac gctgcggatc tcgatcgaca ccaaggccaa ggtgcaggtc
      781 ggggaattct cccgtggcgg ggcggctcgg ggcgcgggac cggtggaggc gctggaccac
      841 gacatgaagc cggtggcgac attggtcccc ttcgggatcc tggaggtggt cagtggtttg
      901 ctgaccatcc tgttcgggac gacgtgcttg acgagtgatt tcgtggtgga ctgtttggag
      961 tactggtggc aggagaggcg caagagcttg tcgcaggtga agaaattggt gatcgatctg
     1021 gataatggcc cggagacgtc gagtcggcgg acgcagttcg tcagacgctt ggtggagttc
     1081 gcggaccggt tcagtctggt gatcgagtta gtgtactatc cgccgtatca cagcaagtac
     1141 aacgcgatcg agcgttgttg ggggatcttg gagaagcact ggaacggggc gttgttatcg
     1201 catgtagaga cggtgctgtg ttgggcaggg acgatgacat ggaagggtca gccaccggaa
     1261 gtgcgattgg tcgagaaaga gtatgagcgt gggaggggtg tggacaagaa ggagatgaag
     1321 cggtacgagg cccgtctgca acgctccgaa gcacttccca agtggagtgt gattatcact
     1381 cctcaagcgt gttaatgggg gagcttatct ctctccgatt gcctaagtaa tgcgcggggc
     1441 aaagggagat cgaaagcgtg gaagaagttc cgcaacctcg tctggtcgtc ccggatcgcc
     1501 tccaactccc caacgaccac cgcattgagt tggtgagcgc cctctggcgc gaaattgcac
     1561 aggcgatcat actttaacca cgtccacaac tgctccaccg gcatcaactc cggggcgtgg
     1621 gcgggcaagg gttcgatgtc cagccgcccc cgagactggt ccaccagttc gcggatcggg
     1681 tcgcccttgt gcatggtccc ttgatcccag atcgcgatga ccggtccggg gagcccctcc
     1741 accgcgcagc cgaggaattc ggccacctcc atgttgctga agtagccgtt gaccagtgtc
     1801 tgataggcca gccccagccg atcccgcaag ggcggcagcc acaaggccgc cgccaccgac
     1861 accttctctc ggtgcccggc cttctgctgg atcaccggcg ggtggccccg cggcgcccaa
     1921 ctgcgtcgca ccaacggcgc catcagcaac ccgctctcgt ccatcagcag caggttcgcc
     1981 ccccgccgcc gcgccttttt ttgatgcggg gccagtcctt ctccagccac cgggcgatgg
     2041 cctcgtcatc ccgctggcgg gcccggcgcc gcggcgtctg gggcgtgaag ccactgctgg
     2101 cggagccagg tggtcaggta atcagggtgg aagtggacgc cgaactcctg ctcgatgagc
     2161 tgggccagcc gtggggccgt ccacaactcg gtggcgaagc cgtgttcgat gggattgtcg
     2221 gccagccaac ggcggacgat tttctcctgg gtcgaggaca gtttgggcgg tcgcccgggc
     2281 acgggttgcg ctgccaggcc gtcggcaccc tgtcgtcgga aggtagcgac ccagcggcgg
     2341 acgctgctgg gagccacgtc gaggaagtca gcgacctctg cgatggagta accctcctcg
     2401 attcgctgga cggcgagctg acgacgatac t
//
LOCUS       DBNGZC010000434         1189 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2288123, whole genome shotgun sequence.
ACCESSION   DBNGZC010000434 DBNGZC010000000
VERSION     DBNGZC010000434.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2288123"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="AC16G0_07665"
     CDS             <1..1115
                     /locus_tag="AC16G0_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HIR5099531.1"
                     /translation="TGGTFGVNYATIGGRLLAFNYTSIYLSEAERKTIHEQFILNHYL
                     KQFNEETIQPKQQATCGEPCMAVCKKLRDEYKKDYEPYQTLGPLCGIFDQRAAERLNR
                     QGDMYGFDTISVGGVLSWLMECLSRGLLTKEELGVRREPIFSPSGFSLERDSAENADI
                     GVGLLDGIIAKRGILNLEEGARKLARRLARERGKAVLDPFVYIAYARKGWMVPNQYWT
                     PGALSPMAIMGKYFMYYGSEFMPPRKLGRINAQRFRDELIMDNMGACRFHRLWAEEML
                     PEVIGSLYGQKEGFLKGLAITAGRINSRNSSVFWESERAADYVHTFMKRHHDVDGNND
                     AELLDWIDRFEKDKSEAALSFWYEVHKGVHESLREF"
BASE COUNT          243 a          364 c          377 g          205 t
ORIGIN      
        1 aaaccggcgg gaccttcggg gtaaattacg ccacgatcgg cggccgactg ctggccttca
       61 attacaccag catctatctt tcggaggccg agcggaagac aatccacgag cagttcatcc
      121 tcaaccacta cctcaagcag ttcaacgagg agaccatcca gcccaagcaa caggccacgt
      181 gcggcgagcc atgcatggcc gtctgcaaga agctgcgcga cgagtacaag aaagattacg
      241 agccgtacca gaccctgggc cccctctgcg gcatcttcga ccagcgggcc gcagagcggc
      301 tgaaccgtca gggggacatg tacggcttcg acaccatctc ggtcggggga gttctttcct
      361 ggctgatgga gtgcctctcg cgggggctcc tcaccaaaga agagctgggc gtgaggcgag
      421 agccgatttt ttcccccagc ggcttcagcc tggagaggga ctcggccgag aatgccgaca
      481 tcggcgtggg actcctcgac ggcatcatcg ccaagcgcgg catcctgaat ctggaggagg
      541 gggcgcggaa gctggcgcgg cggctcgccc gcgagcgggg caaggccgtg ctggatccgt
      601 tcgtgtatat cgcctatgcc aggaaaggct ggatggtgcc caatcagtac tggacccccg
      661 gcgccctctc acccatggcc atcatgggca agtacttcat gtactacggc agcgagttca
      721 tgccgcccag aaagcttggc cggatcaatg cccagcgctt ccgtgacgag ctgatcatgg
      781 acaacatggg ggcgtgccgg ttccatcgcc tctgggccga ggagatgctc ccggaggtca
      841 tcgggtccct gtacgggcag aaggagggct tcctgaaggg gctcgccatc acggccggcc
      901 ggatcaatag ccggaactcc tccgtcttct gggaatccga acgggccgcc gactacgtcc
      961 acaccttcat gaagcgacac cacgatgtgg acggaaacaa cgatgcggag ctgctcgact
     1021 ggatcgaccg gtttgagaag gacaaatccg aggctgccct gagcttctgg tacgaggtgc
     1081 acaagggcgt ccacgaatcc ctgcgggagt tttgattgcc ggggtctggc acgctcaccc
     1141 tggaggagtg cctcaacgcg ctcttccagg acgtcgacgc gtgtgacgt
//
LOCUS       DBNGZC010000435         2279 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1110044, whole genome shotgun sequence.
ACCESSION   DBNGZC010000435 DBNGZC010000000
VERSION     DBNGZC010000435.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1110044"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="AC16G0_07670"
     CDS             <1..506
                     /locus_tag="AC16G0_07670"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diaminopimelate decarboxylase family protein"
                     /protein_id="HIR5099532.1"
                     /translation="AEFAQAMLEVIKDLGATVVLEPGRVLVGNAGILVARVLYSKQNP
                     VKNFLVVDAGMNDLARPSLYGSYHTIRPVRRVPGREEVTVDVVGPICESGDFLAKDRT
                     LPRFEAGEFLAVMSAGAYGHTMSSNYNARPRAPEVMVRGDRYDVVRDRETYEDLVRGE
                     RMLDPLP"
     gene            602..1486
                     /gene="dapA"
                     /locus_tag="AC16G0_07675"
     CDS             602..1486
                     /gene="dapA"
                     /locus_tag="AC16G0_07675"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR5099533.1"
                     /translation="MAKRVFQGSLVALVTPFRNGKVDEPTLKELVEFQIAGGTDAIVP
                     CGTTGESPTLDHEEHRNVIDLVIRAVNKRIPVVAGTGSNSTAEAISLTKHAKAAGADA
                     ALIVLPYYNKPTQKGLIEHCRAIADAVTLPLILYNIPGRTGVNMLPETVAQLAEHPNI
                     VGMKEATGNLEQMTQDIVLCGDKLSFLSGDDTLTLPLMSVGGRGIISVVANIVPKDVA
                     DMTHAFLSGDWKRARELHLKLFPLSQAMFIETNPIPVKTALAMMGKIREEFRLPLCSM
                     VEANRKKLEAALRNYGLL"
     gene            1570..>2279
                     /gene="dapB"
                     /locus_tag="AC16G0_07680"
     CDS             1570..>2279
                     /gene="dapB"
                     /locus_tag="AC16G0_07680"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR5099534.1"
                     /translation="MIKAVVTGAGGRMGKRLVALLRESAEFQVVGAVERKDHVDLGRD
                     AGEVAGVGPSGVPIVDALANALPDAQVVLDFTAPAAAMQHLEVASRSGVAMVLGTTGL
                     TEADLRKAKDLCARIPCVQSPNMSVGVNVLYGVLAQVARTLGEGYDIEVIEAHHHFKK
                     DAPSGTADRMAKVLAEALGRDLKQVGVYGRHGLVGERKQTEIGVHAIRAGDIVGEHTI
                     LFGGMGERIEIIHRAHSR"
BASE COUNT          427 a          685 c          808 g          359 t
ORIGIN      
        1 ccgccgaatt cgcgcaagcg atgctggaag tgatcaagga cctgggcgcc accgtggtcc
       61 tggagccggg gcgggtcctg gtcggcaatg ccggcatcct ggtggcccgc gtcctgtatt
      121 ccaagcagaa cccggtgaag aacttcctgg tggtggacgc ggggatgaac gatctggccc
      181 ggccgagctt gtacggctcc tatcatacca tccggccggt gcggcgagtt cctggccgag
      241 aggaggtgac ggtggatgtc gtggggccga tctgcgagtc cggtgacttc ctggccaagg
      301 atcgcaccct gccccgcttc gaggcgggcg agttcttggc cgtgatgtcg gcgggggcgt
      361 acggccatac catgtcatcc aactacaatg cccgaccgcg ggcacccgag gtgatggtcc
      421 ggggggaccg gtacgacgtc gtgcgggatc gcgagaccta cgaggacctc gtccgaggag
      481 agcggatgct ggatccgttg ccgtgaagcg ggtcggcgaa cggggcagtg ccggcgtttc
      541 cgcgccagcg gcgccgagcg cgagggacaa gcgcaacagg gcttgatggg gaggtatggc
      601 catggcaaag cgcgtctttc agggttcgct ggtggctctg gtcaccccgt tccgtaacgg
      661 gaaagttgac gagcccacgc taaaggaatt ggtggaattc cagatcgccg gagggaccga
      721 tgccatcgtt ccttgcggga cgacgggcga gtcccccacc ctggaccacg aggagcaccg
      781 gaacgtgatc gacctggtga tccgggccgt caacaagcga attccggtgg tggcgggcac
      841 ggggtcgaac agcacggcgg aggctatcag cctcaccaag catgccaagg cagcgggagc
      901 ggacgcggcc ctgatcgttc tcccctatta caacaagccg acccagaagg gcctgatcga
      961 acactgccgg gccattgccg acgcggtaac cctgccactg atcctgtaca acattccggg
     1021 gcggaccggc gtgaacatgc tgcccgagac cgtggcccag ctcgccgagc atcccaacat
     1081 cgtcgggatg aaagaggcca cggggaatct ggagcagatg acccaggaca tcgtcctgtg
     1141 cggcgacaag ctgagcttcc tctcggggga cgacaccctg accctgccct tgatgtccgt
     1201 gggcggccgg gggatcatct ccgtcgtggc caatatcgtg cccaaggacg tggccgatat
     1261 gacccatgcc ttcctgagcg gggactggaa gcgggcccgc gagctgcacc tcaagctgtt
     1321 ccccctgtcc caggccatgt tcatcgagac caaccccatt ccggtgaaga ccgccttggc
     1381 catgatggga aagatcaggg aggagttccg gctcccgtta tgttccatgg tcgaagccaa
     1441 tcggaagaag ctggaggcgg ccctccgaaa ctacgggctg ctgtgaggga acatcgacag
     1501 ggacagtgac aggcttaggc cgagaaagcc tgaggagtcg ccgacgccgc cactgaggga
     1561 ggcagggccg tgatcaaggc cgtcgtcacc ggtgccggag ggcgaatggg caagcgcctg
     1621 gtcgcccttc tgcgggagag cgcagagttt caggtcgtgg gggcagtgga acggaaagac
     1681 cacgtggacc tggggcggga cgcaggtgag gttgccgggg tcggcccctc gggagtgccc
     1741 atcgtggacg cgctcgccaa tgcgctgccc gacgcccagg tagtcctgga cttcacggcg
     1801 cccgcagcgg ccatgcagca tctggaggtg gcgagccgct ccggagtcgc catggttctg
     1861 ggcacaaccg gtctcaccga agcggacctg cgcaaggcca aggacctatg cgcccggatt
     1921 ccctgcgtgc agtcgccgaa catgagcgtt ggagtcaacg tcctctacgg cgtcctggcg
     1981 caggtggccc ggacgctggg tgagggatac gacatcgagg tgatcgaggc gcatcaccac
     2041 ttcaagaagg acgcccccag cggcaccgcg gaccgcatgg cgaaggtctt ggccgaagcc
     2101 ctggggcgcg acctcaagca ggtgggtgtg tacggccgtc atggtttggt aggggagcgg
     2161 aaacagacgg agatcggagt ccacgccatc cgggccgggg acatcgtggg ggagcacacc
     2221 atcctcttcg gcgggatggg cgagcggatc gagatcatcc atcgggcgca cagtcgcga
//
LOCUS       DBNGZC010000436         3384 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2481795, whole genome shotgun sequence.
ACCESSION   DBNGZC010000436 DBNGZC010000000
VERSION     DBNGZC010000436.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2481795"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="AC16G0_07685"
     CDS             <1..582
                     /locus_tag="AC16G0_07685"
                     /inference="COORDINATES: protein motif:HMM:NF014695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HIR5099535.1"
                     /translation="ALLEPFTAGLRGGALEAGLHWLYTASGFMGIGFAMLGAVALLIR
                     RLTAGDLKTYSTPGDIFNLCAFILCFGCLLAGYLLRGSESPGTVAVAQAILTFDTSVR
                     IPGTFLVALALSALMVAYIPFTHMSHFIGKFFTYHMIRWNDQPNLPGGMLEKKLAEYL
                     TYRPTWAAPHVGAAGTKTWADVVSINPAQGARK"
     gene            579..2186
                     /locus_tag="AC16G0_07690"
     CDS             579..2186
                     /locus_tag="AC16G0_07690"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008684768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR5099536.1"
                     /translation="MKEKISLDDMARKDREPLVHVKNGDLMPLPPPLEEPAKDRVWTP
                     LSAQAREKYECLLDDTCAVNIPRPKTQEEERNLLQRFLSGLDNLFSRENNWAFLQPLL
                     LTMEYCAKCQTCSPACHVFEASGGNELYRPMYRSEILRRLYFKYVRGGGLLSAWQHGD
                     IELNWPLVARLIELAYRCNLCRRCAQTCPMGADNGLVAHELRKLFSMEMGIAPKEIHG
                     NGSMLQLKVGSSTGMSPAVVKDNIAFIDEDTTERTGVSVETPWDVEGAEVLLLHNAGE
                     IMAWPENPGAFALILTAAGIKWTLSSEVAGYDAINYGLWYDDAQFARVAIKHAEAARK
                     LKVKKIVLGECGHANKALAVVGDRILTGDLNIPRESAMTLLRDIVMQDRLALDPRRND
                     FPVTLHDPCNLVRLMGVVEPQREILRKICPGFREMHPHGVDNYCCGGGSGFAIMSGNN
                     FPDWRFHVSGRKKLQQILTAFADCLPPETPKYVCAPCSNCKGQLRDMFAYYRLWENHR
                     ILYGGLVELIVNAMQDVKPGFIQWEWH"
     gene            2300..3091
                     /locus_tag="AC16G0_07695"
     CDS             2300..3091
                     /locus_tag="AC16G0_07695"
                     /inference="COORDINATES: protein motif:HMM:NF021252.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HIR5099537.1"
                     /translation="MKNQRRDVLKMFAAASGAAALTPFLGRSLQATAVTDAIPAVPWP
                     YKKLNLELAAERGYAGYYKGACCYGAFDAIISQLREEVGPPYTMMPSEMMIFGEGGIA
                     GISSVCGALLGASAAIFLVAGGLDPKKREEAFPLIRELFAWYEQEALPSYRPKNPKFE
                     IKTSVARSPLCHASVTRWSKATGFKSFSKERSERCGWLTASVAKYTADMLNTTLLDAS
                     FKPVHALSSEVKACRGCHDKGGAVENSRGLMDCGGCHFTGKAKHP"
     gene            3177..>3384
                     /locus_tag="AC16G0_07700"
     CDS             3177..>3384
                     /locus_tag="AC16G0_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099538.1"
                     /translation="MGQEEIKTAAKRILGYLEAHGDAPILAMKNDLKNPELQFYMGLG
                     DLIREHRVTLQERQGAFWTTPCSKS"
BASE COUNT          672 a         1096 c         1029 g          587 t
ORIGIN      
        1 gccctcctcg aacctttcac cgccggcctc aggggaggcg cgctggaggc aggtctccat
       61 tggctctaca ccgcctcggg attcatggga atcggcttcg ccatgctggg agcagtcgca
      121 ctgctcatcc gacgactgac ggcaggggac ctcaagacct acagcactcc gggcgacatc
      181 ttcaacctgt gtgcattcat cctgtgcttc ggctgcctgc ttgcaggcta cctcctcagg
      241 ggctcggagt ccccaggcac cgtcgccgtc gcgcaagcca tcctcacctt cgacaccagc
      301 gtcaggatcc ccgggacgtt ccttgtggcc ctcgccctgt cggctctcat ggtcgcgtac
      361 atccccttca cccacatgtc gcacttcatt ggcaagttct tcacctacca catgatccgc
      421 tggaacgacc agcccaacct gccgggcggc atgctcgaaa agaaactcgc cgaatacctg
      481 acctaccggc ccacgtgggc cgcgccgcat gtcggcgccg ccgggacaaa gacctgggcg
      541 gatgtggtca gcatcaaccc cgcacagggg gcaaggaaat gaaggagaag atcagcctgg
      601 atgacatggc ccggaaggac agggagcctc tcgttcacgt gaaaaacggc gacctcatgc
      661 ccctcccacc ccccctggaa gaacccgcca aggatcgtgt ctggacgccg ctctcggccc
      721 aagcccggga gaaatacgag tgcctcctgg acgatacatg cgccgtcaac atcccccgac
      781 cgaagaccca agaggaggag aggaatctcc tccagagatt tctgtccggt ctggacaacc
      841 tcttctcccg agagaacaac tgggccttcc tgcagcccct gctgctgacc atggagtatt
      901 gcgccaagtg ccagacgtgc tctccggcct gccacgtctt cgaggccagc ggcgggaatg
      961 agctgtaccg ccccatgtac cgttccgaga tcctgcggcg cctgtacttc aagtacgtcc
     1021 ggggcggggg tctcctctcc gcctggcagc acggggacat cgagctgaac tggcccctgg
     1081 ttgcccgcct gatcgagctg gcctatcggt gcaacctgtg ccgtcggtgc gcccagacct
     1141 gtcccatggg ggcggacaac ggtctcgtgg cccacgaact ccgcaagctg ttcagcatgg
     1201 agatgggaat cgcgcccaag gagatccacg ggaacggctc catgctccag ctcaaggtgg
     1261 gctcctcgac cggcatgagc cccgcggtgg tcaaggacaa catcgcattc atcgacgagg
     1321 atacgaccga gcgcaccggc gtctccgtgg agaccccatg ggatgtggag ggggccgagg
     1381 tgctcctgct ccacaacgcc ggcgagatca tggcctggcc cgagaacccc ggcgccttcg
     1441 ccctcatcct gaccgccgcc ggaatcaagt ggaccctctc atccgaggta gccggctacg
     1501 atgccatcaa ttatgggctc tggtacgacg acgcgcaatt tgcccgtgtg gccatcaagc
     1561 acgccgaggc cgccaggaag ctcaaggtca agaagatcgt cctgggcgag tgcgggcacg
     1621 ccaacaaggc cctggccgtc gtcggggacc ggatcctcac cggcgatcta aatatcccgc
     1681 gggagagcgc catgacgctc ctgcgcgaca ttgtcatgca ggatcggctc gcgctggatc
     1741 ctcgccgtaa cgacttcccg gtcacgcttc acgatccgtg caacctggtg cgcctgatgg
     1801 gtgtggtgga gccccaacgg gagattctcc ggaagatctg tcccgggttc cgcgagatgc
     1861 acccgcacgg cgtggacaac tactgctgcg gcggaggaag cggttttgcc atcatgagtg
     1921 gcaacaactt cccggactgg cgcttccacg tgtccggccg caagaaactc cagcagatcc
     1981 tcacggcgtt cgccgactgc ctcccacccg agacgccgaa gtacgtctgc gccccctgca
     2041 gcaactgcaa gggacagctc cgggacatgt tcgcctatta tcgcctgtgg gagaatcatc
     2101 ggatcctgta cggcggcctg gtggaactca ttgtcaacgc catgcaggat gtgaagcccg
     2161 gcttcatcca gtgggaatgg cattgaggaa tgatcacatc ccagcaggct ctcacggccc
     2221 actgggatgc ggtcactgga cacacgccac ccggcgtcga aagtggagaa tcgcgctgct
     2281 ttgaaaggag aaggaccgca tgaagaatca acggcgagac gtgctcaaga tgttcgcggc
     2341 ggcatcgggc gcggccgcac tcacaccatt cttggggagg agcctgcaag ctacggcggt
     2401 gacggatgcg atccccgcag ttccctggcc ctacaagaaa ctgaatttgg aactcgcggc
     2461 ggagcgcgga tacgcaggct actataaggg agcatgctgc tacggggcct tcgatgcaat
     2521 catcagccag cttcgcgagg aggtggggcc tccgtacacg atgatgccgt cggaaatgat
     2581 gatcttcggg gaggggggta ttgcgggcat ctcctccgtg tgcggcgccc tgctcggcgc
     2641 ctccgcggcc atttttttgg tcgcgggagg gctcgaccca aagaagcgag aggaggcatt
     2701 cccgctgata cgcgagctgt tcgcctggta cgagcaggaa gccctcccca gctaccggcc
     2761 caagaatccg aagttcgaga tcaagacttc cgtcgcacgc tccccgctct gccatgcctc
     2821 ggtgacgcgc tggtcgaagg cgacgggctt caagtcattc tcgaaggagc ggtcagagcg
     2881 gtgcggctgg ctcaccgcct ccgtggcgaa gtatacggca gacatgctga acaccaccct
     2941 gctcgacgcc tccttcaagc ccgtccacgc cctctcgtcg gaggtgaagg cgtgccgtgg
     3001 ctgccatgac aagggcgggg cagtcgagaa cagccgtggc ctgatggact gcggtgggtg
     3061 tcacttcacg gggaaggcga agcatcccta actgagccac catgaccaag gagccaatga
     3121 ccaaatctca atggattctc ggcgcgcatg gatcaggagg agcggccgtt gagaagatgg
     3181 ggcaggagga gatcaagacg gcagccaagc gaatcctggg ttacctagag gcccacgggg
     3241 atgcccccat tctcgccatg aagaatgacc tgaagaaccc cgagctccag ttttacatgg
     3301 gcttgggtga cctcatccgt gagcaccgcg tcaccctcca ggagcgtcag ggggcgttct
     3361 ggactacacc ctgctcgaaa tcca
//
LOCUS       DBNGZC010000437         5070 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_135377, whole genome shotgun sequence.
ACCESSION   DBNGZC010000437 DBNGZC010000000
VERSION     DBNGZC010000437.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_135377"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="AC16G0_07705"
     CDS             complement(<1..267)
                     /locus_tag="AC16G0_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019644195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR5099539.1"
                     /translation="MQSFGGLLHGFSVALSPLNLLFGFLGAVIGTIIGILPGLGPLGT
                     MALLLSLTYGMDPVAGMILFAGIYFGAMYGGSTTSILLNIPGEAA"
     gene            complement(282..812)
                     /locus_tag="AC16G0_07710"
     CDS             complement(282..812)
                     /locus_tag="AC16G0_07710"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR5099540.1"
                     /translation="MRLPSSDRAGRPVKKADALLAGIVLLIGIGYEGMAWWMPRGSIS
                     YPGPGFFPVIVGLFLILTAAGCVIQAFTAGMSPNPSAAGGKLAGEHRPTTRPSAGKAW
                     ALLAFLVAYALLLKPLGFLIAIFLFLLASIRIFGYRRWLPSLGFTAAIAALSYVSFVI
                     WLKVPLPLGVLGDILD"
     gene            complement(840..1811)
                     /locus_tag="AC16G0_07715"
     CDS             complement(840..1811)
                     /locus_tag="AC16G0_07715"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR5099541.1"
                     /translation="MRTKIAALVVTLVVTLAWAGFPKAAEYPRKPINVLIGFAPGGGS
                     DVMLSMVRPLLEKTLKTTLVPVYKPGAGSDIALTELAGSKADGYTVVISCTPQVPINP
                     VVRETQYKLSDMLFVANVVTDPGILVVRTESPYKTLADLLQAMKEKPGKIGVGVSSAP
                     GDDWFAVHMLQAAAKVEANIVPFSGDGPSWQAALAGHVDASANNLGIVYPQVKGGKLR
                     ALAIMTEKRSPYLPDVPTFKELGYNFTSGSSRGFSMPKETPKEIVQTFANAVKQVMET
                     QEFKANADKTAFPSDYQGPEEYTAYIKKLDVVYRPLWDKYGKTAVVK"
     gene            complement(2193..2942)
                     /locus_tag="AC16G0_07720"
     CDS             complement(2193..2942)
                     /locus_tag="AC16G0_07720"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5099542.1"
                     /translation="MDLGIDGKVAVITGAGSGIGRATALLFARWGARVTGVDIDADAL
                     EAVARDIREIGSEMETALANVCAEEPVVRFYETLGRKHGRLDILVNVVGGGRPATLPE
                     ITGAEWDRMFSFNAKSCFLMSREAVRYMTQGGRIVNVASLAGQSSSVLQGAHYSASKA
                     AVIGLTRHMARELGPLGIAVNAIAPGTTATDRIQRQMTPERRQAVISRIPLGRLATPE
                     DQAGVILFLASSLASYVTGVTIDVSGGMLLG"
     gene            3313..4230
                     /locus_tag="AC16G0_07725"
     CDS             3313..4230
                     /locus_tag="AC16G0_07725"
                     /inference="COORDINATES: protein motif:HMM:NF024874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5099543.1"
                     /translation="MDADKIDRTLRSARIWWDRLTRPPDPEEKEARDSPRFIAPVRPN
                     PRTWSDGGITLAWVGHSTFLINLEGTTILTDPVFARKVGISLLGLMTVGIERFVPPAL
                     TFQDLPPVDLVLVSHAHMDHYDIPSLRTLPRDVPMILARDTTEFIDGLGFSKVQELDW
                     GQTAEVAGVRIEAMPVKHWGSRYPWDRDRGYNGLLLQKHKRSILFGGDTAYTELLPAA
                     LNHRPIDVALLPIGGYNPYIANHANPEQAWHLFHEMRARYFIPMHWRTFRMSYEPPFE
                     PFERLATAVNGSAPRIALHTIGETWSLPG"
     gene            4380..>5070
                     /locus_tag="AC16G0_07730"
     CDS             4380..>5070
                     /locus_tag="AC16G0_07730"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR5099544.1"
                     /translation="MRRLQGLVIATLTPMHEDGSLDQPGFAAHVQDLLENGAEGLFVA
                     GTTGEGLLLDEEERLEVVRAAVRLVAGRVPVVGLCGGLTTAGAVRLAVRMRDAGADGV
                     AALTPFFYHVDVEAMAEHFRRVADAAQCPTYLYSIPGLTGVSLPVEVLERLRVHPCFG
                     GLKFSYCDLEQLGKYVRTGAPVFIGCDSLITQALRIGAAGTVSGTAACLPAPFATLFA
                     RIRQGGDSSAAQ"
BASE COUNT          901 a         1637 c         1648 g          884 t
ORIGIN      
        1 ggccgcctct cccgggatgt tgagcaggat ggaggtggtc gatcctccgt acatggcgcc
       61 gaagtagatg ccggcgaaca ggatcatgcc ggccaccgga tccataccgt aggtcagtga
      121 caggagcagg gccatggtcc ccaggggacc gagaccgggc aggattccga tgattgtccc
      181 gatgacggcg cccaggaacc cgaacagcag gttcagtggg gacagggcca ccgagaaccc
      241 gtgcaggagc ccaccgaacg attgcatgtc gcgcgctcct cctagtccag gatgtcgccg
      301 agaacgccga gggggagcgg caccttcagc cagatcacga aggagacata ggagagcgcc
      361 gcgatggccg ccgtgaatcc gagtgagggg agccatctcc ggtaaccgaa gatgcggatg
      421 gacgccagca ggaagagaaa gatcgcgatc aggaatccca gaggcttgag gaggagcgcg
      481 tacgccacga ggaaggcgag aagcgcccat gccttgccgg cggagggcct tgtggtcggc
      541 cggtgctccc ccgcaagctt gcccccggct gcggaggggt taggcgacat cccagcagtg
      601 aaggcctgga tcacgcagcc ggcggcggtg aggatcagga atagccccac gatcaccggg
      661 aagaacccgg gaccgggata ggagatgctt ccccgtggca tccaccaggc catgccttca
      721 tatccgatcc cgatgagcag gacgatccca gccagcagcg catccgcctt cttcaccggt
      781 ctccccgcgc gatccgagga cgggaggcgc actcgtgcct cccgtcctcg aaaaagccgc
      841 tacttcacga ctgcggtctt gccgtacttg tcccacaggg gtcggtacac aacgtccagc
      901 ttcttgatgt aggcggtgta ttcctcggga ccctgatagt cggaggggaa ggccgtcttg
      961 tcggcgttgg ccttgaactc ctgggtctcc atgacctgct tcacggcatt ggcaaaggtc
     1021 tgcacgatct ctttcggggt ctctttcggc atggagaagc cgcgggacga cccgctggtg
     1081 aaattgtagc ccagctcctt gaaggtgggg acatccggca ggtaggggga gcgcttctcc
     1141 gtcatgatgg cgagcgcccg aagcttgccg cccttcacct ggggatacac gatgcccagg
     1201 ttgttggcgg aggcatccac gtggccggcc agggccgcct gccaggacgg gccgtcaccc
     1261 gagaacggca cgatgttggc ctcgaccttg gctgcggcct ggagcatgtg cacggcgaac
     1321 caatcatccc ccggggcgga ggagaccccc acgccgatct tccccggctt ctccttcatc
     1381 gcctggagga ggtcggcgag ggttttgtag gggctctccg tgcgcaccac caggatgccg
     1441 gggtcggtga ccacgttggc cacgaacagc atgtccgaga gcttgtactg ggtctcccgc
     1501 acgacggggt tgatcggcac ctgcggtgtg caggagatca cgacggtata cccgtcagcc
     1561 ttggatcccg ccagctccgt caaggcgatg tcgctcccgg cgcccggctt gtacacgggt
     1621 accagggtgg tcttgagcgt cttctccagc aggggacgca ccatggagag catgacatca
     1681 ctgccaccgc cgggggcgaa gccgatgagg acgttgatgg gttttctcgg gtactcggca
     1741 gccttcggga atcctgccca cgccagcgtc acgaccagcg tcacgactag ggccgcaatc
     1801 ttcgtacgca tatccttcga cctcccgtca tggaatgcga gacgtcacaa ccccgttgca
     1861 cctctctggt cctcctttcc ccagggtgat tcccctccca tcaaggtcct tgtacaaact
     1921 ttcttccggc gatgcaaaag ggaaaatagc cagagacccg gtatgcttcc aaaccctgac
     1981 aagttcacat ctcctgacat gggtgagctt gggtacaaaa tcctggacct ctcgccccgg
     2041 tgggccggcg gtgagtgaca cggatcctcc agtccatcag cgactccaga cctacggaca
     2101 cacgtcatga gtccgataat ggcggtgggt ggggaattcg agcgcggagc gtcggcggac
     2161 gcgcgctgcg tcattgggaa gttaggggga ggttagccga ggagcatccc gccgctgacg
     2221 tcgatcgtga ccccggtcac gtaactggcc aaggaggagg ccaggaacag gatgacccct
     2281 gcttgatctt cgggagtggc caatcttccg agcgggatgc gggagatcac agcctggcgc
     2341 ctctccgggg tcatctggcg ttggatgcgg tcggtggccg tcgtccccgg ggcgatggcg
     2401 ttgacggcga tacccagggg gcccagctcg cgggccatgt gccgggtcag cccgatcacc
     2461 gccgccttgg atgcgctgta atgcgccccc tgaaggacgc tgctgctctg tcctgccagt
     2521 gaggcgacgt tgacgatccg cccgccttgg gtcatgtagc gaacggcctc ccgggacatc
     2581 aggaagcagc tcttggcgtt gaagctgaac atgcggtccc actccgcccc cgtgatctcc
     2641 gggagcgtgg caggcctgcc gccccccacc acattgacca ggatatcgag tcgcccgtgc
     2701 ttccggccga gcgtctcgta gaagcggacc accggctcct cggcgcagac attcgccagg
     2761 gccgtctcca tctcgctgcc gatctcccgg atgtccctgg ccaccgcctc cagagcgtcg
     2821 gcgtcgatgt ccacccccgt caccctggcg ccccatcggg cgaataggag agccgtcgcc
     2881 cgcccgattc cgctcccggc accggtgatg acggcgactt tcccgtcgat acccaggtcc
     2941 atttccgagg tgcgaccaga ttcagacaag gggattctcc atcaggaacg gcacccccac
     3001 atacccatgc aaaggcggcg ggagacgcga gatgccacac tcatagggga tagcgcgaca
     3061 caagtcaagc gtgaagggtc ccaccagcat gaggcagggc aggttgccag ttttcctgtt
     3121 gcacgccctc cggtcctcag ccatactttt tgtgttgctt gtatgttcac acggaacggt
     3181 tgagccatgc ggcgaaaatc cggatggaaa atctccccgg ccgagcggga ccgcgcgagg
     3241 cggcgcttcc tgtccctggt cctcatgaca ggagcgctga cgtgcgtctc tcggaaggcg
     3301 tgggcacaga caatggacgc agacaagatc gatcggaccc tgcgaagcgc gcggatctgg
     3361 tgggaccggc tgacccgccc accggatccg gaggagaagg aagccaggga ctcaccacgc
     3421 ttcattgcac cggttcgtcc aaatccccgc acgtggtccg acggcggcat caccctggcc
     3481 tgggtgggcc actccacatt tctgatcaac ctggagggca cgacgatcct gaccgacccg
     3541 gtcttcgccc ggaaggtggg catcagcctc ctggggctca tgaccgtagg gatcgaacga
     3601 ttcgtgccgc cggcgctcac gttccaggat cttccccctg ttgacctggt gctcgtctcg
     3661 cacgcccaca tggatcacta cgacatcccc tcgctgcgaa cgctcccgcg agatgtcccg
     3721 atgatcctgg cacgggacac caccgagttc atcgacgggc tgggattctc gaaggtgcag
     3781 gagctagatt gggggcagac cgccgaggtg gccggcgtcc ggatcgaggc catgccagtt
     3841 aagcactggg ggagccgcta tccctgggac agagaccggg ggtataacgg gctcctcctc
     3901 cagaagcaca agcgaagcat cctcttcggc ggcgacacgg cctacaccga actgctcccc
     3961 gctgccctga accatcgccc gattgacgtg gcgctcctcc ccatcggcgg ctacaacccg
     4021 tacatcgcca accacgccaa tccagagcag gcctggcacc tcttccacga gatgcgcgcc
     4081 cgctacttca tccccatgca ctggcggacg ttccggatgt cctacgagcc ccccttcgag
     4141 ccgttcgagc gtctggcgac tgccgtcaac gggtccgccc cgcggatcgc cctgcatacc
     4201 atcggcgaga cctggagtct gcccggctag agtcggtctc cgggggggcg cccaggctgt
     4261 tgccccctga ctgccgcgca gcccatgggt gaccgatttg aagggggcgg tgcgaaaagg
     4321 gaggtctttc cgcaggtcga tcggacaggg attcttctcc gggtaagagg gagtcaagga
     4381 tgaggagatt gcaggggctg gtgatcgcca cgctcacgcc gatgcacgag gatggctctc
     4441 tggaccagcc aggcttcgcc gcgcacgttc aggatctcct ggagaacggg gcggaaggcc
     4501 tattcgtggc cgggacgacg ggggagggac ttcttctgga cgaggaggag cgactggagg
     4561 tcgtccgggc ggcggtgagg ctggttgccg gccgcgttcc cgtggtgggt ctctgcggtg
     4621 gtctcaccac ggccggggcg gtgaggttgg cggtacgcat gcgcgacgcg ggggcggatg
     4681 gggttgccgc gctcacgccg ttcttctacc acgtggatgt cgaggccatg gcggagcatt
     4741 ttcgccgggt ggcggacgcg gcccagtgtc cgacgtacct ttacagcatt ccggggctca
     4801 caggcgtctc gctccccgtc gaggtactgg agcggctgcg cgtccatccc tgctttggcg
     4861 gcctgaagtt cagctattgc gacctggagc aactgggcaa gtacgtccga acgggcgccc
     4921 cggtgttcat cggttgcgac tccctcatca cccaggccct ccgcattggc gcggccggca
     4981 cggtgtcagg aaccgcggcc tgtctgcccg caccctttgc cacgctcttc gcccgcatcc
     5041 ggcagggcgg ggactcctcg gccgcacagg
//
LOCUS       DBNGZC010000438         2624 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_135378, whole genome shotgun sequence.
ACCESSION   DBNGZC010000438 DBNGZC010000000
VERSION     DBNGZC010000438.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_135378"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="AC16G0_07735"
     CDS             complement(<1..302)
                     /locus_tag="AC16G0_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HIR5099545.1"
                     /translation="MYTDRENGAAIRRRVLVDGLSQRAACREFDIHWDTLQKILDHPE
                     PPGYRRTASRPKPKLEAFLPVIHQILQDDKKAPKKQRHTAKRIFERLRDEYGYTGGL"
     gene            complement(673..2379)
                     /locus_tag="AC16G0_07740"
     CDS             complement(673..2379)
                     /locus_tag="AC16G0_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006424230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HIR5099546.1"
                     /translation="MFFRTKTSGSRSYLQIVENRWEDGRTRQRVVVTLGRLDQLQQDG
                     RLDALLASGARLSQSVLLLSAHAKGQLPTITTRHIGPALIFERLWQQTGCQHVIEQLL
                     DERRFECDVERAIFLTVLHRLFAPGSDRAADKWKTDYQIDGCEPLQLHHLYRAMAWLG
                     EQLPQNQQKDKTPFAPRCIKDRIEEQVFAHRRDLFTDLQLVFFDTTSIYFEGEGGQDL
                     GQRGFSKDHRPDLYQMVVGAVLDGQGRPICCELWPGNTTDVTTLIPVVDRLRSRFGVR
                     RVCVVADRGMISQETIEALEQPGRNWQYILGARMRSQTEVKDEVLSRAGRYRVVHPPR
                     VQSDESSPLKVKEVWVGDHRYVVCLNEDEARKEASDREAIVAALREQLKQGDKALVGN
                     KGYRRYLSGSHPSHFRIDEAKIAEEARYDGKWVLRTNTELDSAEVALQYKQLWMVEHW
                     FRSCKSLLQTRPIYHRRDETIRGHVFCSFLALMLRQELQAQLEERGHELEWADVIGDL
                     DRLQVVEVEQEGKRFLLRSEVQGSCGKVFQAVGVAVPPSVQQVSPTTPGRDAAPGATP
                     PD"
BASE COUNT          472 a          826 c          814 g          512 t
ORIGIN      
        1 aggccgccgg tgtagccgta ctcgtcgcgg agccgctcga agatgcgctt ggcggtgtgc
       61 cgctgcttct tcggggcctt cttgtcgtcc tggaggatct ggtggatgac ggggagaaac
      121 gcctcgagct tcggcttcgg gcgtgaggcc gtgcggcggt agccgggcgg ctcggggtga
      181 tcgaggatct tctggagcgt gtcccagtgg atgtcgaact cacggcaggc ggcgcgctgg
      241 ctcaggccgt cgacgaggac gcggcggcga atcgcggccc cgttctccct atcggtgtac
      301 acccattgac ctcacggctg agggacctgg atggggtgag cgaatccccc atcgtggtcc
      361 cgaggccgtg agcgaggcca tgggcgctcc gttttctgac cgaccgccgg ccgccggcct
      421 gcgctccgtt ttctcagcgg ccgtcacata aagagttgag gacgagatgg acgataaatg
      481 acgtggtcgc tcatgatcag aaatatttct cttttctgat ctggtagcca gcaccaaaac
      541 agactgggca cctgtcatat ccagttgcac ctctcattcc atgcctatga cattcgaagc
      601 agatcgtcca atccttggct caactttaac accacaatgg agcatcttca gcagtggcaa
      661 gaacttgacg catcaatcag gcggcgtggc accaggcgca gcatcccgcc ccggagtcgt
      721 aggcgagact tgttgaacgc tcggcggcac ggctacccct accgcctgga acaccttgcc
      781 acaactcccc tggacctcgc tccgcagcag gaaccgcttc ccctcctgct ccacttccac
      841 cacctgcaaa cggtccaggt ccccgatcac atcggcccac tccaactcgt gaccccgctc
      901 ctccaactgg gcctgaagtt cttgacgaag catcaacgcc agaaacgagc agaacacatg
      961 gccacggatt gtctcatcac gccggtggta gatcggacgg gtttgtaaca acgatttgca
     1021 ggagcggaac cagtgctcca ccatccagag ctgtttgtac tgcaacgcca cctcagccga
     1081 atcgagttcg gtgttggtgc ggaggaccca cttgccgtcg taacgggctt cttcggcgat
     1141 cttcgcctcg tcgatacgga agtgggaggg gtggctgccg ctcaggtaac ggcggtagcc
     1201 cttgttgccg accagcgcct tgtccccttg cttcagttgc tcccgcagcg ccgccacgat
     1261 ggcctctcgg tcggaggctt ccttgcgggc ctcgtcctcg ttgaggcaga ccacgtagcg
     1321 gtggtcgccc acccacacct ccttgacctt caggggcgag gactcgtcgc tctggactcg
     1381 gggcggatgc accacccgat agcgtccggc ccgggatagc acctcgtcct tcacctcggt
     1441 ctgcgatcgc atccgggcac caaggatgta ctgccagttc cgcccaggtt gctccagggc
     1501 ctcgatcgtc tcctggctga tcatgccccg gtccgccacg acgcagaccc gccgcacccc
     1561 gaatcgactc cgcagccgat ccacgacggg gatcaacgtc gtcacgtccg tggtgttgcc
     1621 gggccacagt tcgcagcaga tgggcctccc ctgcccatcg agcaccgcac cgacgaccat
     1681 ctggtagagg tcgggccggt ggtccttgct gaagccccgc tggccgagat cctgaccacc
     1741 ctcgccctca aagtaaatcg aggtggtgtc gaagaagacc agttgcaggt cggtgaagag
     1801 gtctcggcgg tgggcaaaca cctgctcctc gatccggtcc ttgatgcatc ggggggcgaa
     1861 cggtgtcttg tccttctgct gattctgggg caactgctcg ccgagccagg ccatagcccg
     1921 gtagaggtgg tggagttgca agggttcaca cccatcgatc tggtagtccg tcttccactt
     1981 gtcggcggct cgatcgctgc ccggggcgaa gaggcgatgg aggacagtga ggaagatggc
     2041 ccgctcgacg tcacactcga agcggcgttc gtccaggagt tgctcgatga cgtgctgaca
     2101 gccggtctgt tgccagaggc gctcgaagat cagtgccggt ccgatgtggc gggtggtgat
     2161 ggtggggagt tggcccttgg catgagcgga gagcagcagt acggactggg acaggcgtgc
     2221 ccccgaggcc aggagggcat cgagtcgtcc gtcctgctgg agttgatcaa gtcgtcccag
     2281 ggtgacgacg acccgctgtc gggtgcgtcc gtcctcccag cggttctcga caatctggag
     2341 gtaggagcgt gaaccggagg tcttggtgcg aaagaacatg gtgccctcat gggtagggtc
     2401 accatgatca tagggctttc caaggatttt tcaagggcga cctggttatg atgtggcacc
     2461 aggattgtgc ctccgctaca atccctgtcc catcaccaaa ggagttacgt cgaaaacgat
     2521 ggcacaactg tcgaaaaaaa gcttgagcaa cttcccgtac cccagcgcgg gcctgccgaa
     2581 cgagaccccg cgtcctctgg ccgccgcctg gccggcgcgg atgc
//
LOCUS       DBNGZC010000439         2498 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2481956, whole genome shotgun sequence.
ACCESSION   DBNGZC010000439 DBNGZC010000000
VERSION     DBNGZC010000439.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2481956"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="AC16G0_07745"
     CDS             <1..200
                     /locus_tag="AC16G0_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099547.1"
                     /translation="TLGFSGFDQGYIAVEAAHRFLKGEKLPQQIILPVPIVDKSNAEP
                     YNIPPEQRKPPVWDQVLATQK"
     gene            504..917
                     /locus_tag="AC16G0_07750"
     CDS             504..917
                     /locus_tag="AC16G0_07750"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5099548.1"
                     /translation="MKAKNLKRVVRVESVPRYEAPAPYRRFAQALVDQESFPETSMCV
                     GYFTYPPGAKSHTHHHTAENEIYFVLQGELTSVQNGSAFRVPRHGLVFIPKGVSHYAA
                     NRTRRRCVFLAVHAPAVPDLLEFKRSWKRTARQRS"
     gene            921..1625
                     /locus_tag="AC16G0_07755"
     CDS             921..1625
                     /locus_tag="AC16G0_07755"
                     /inference="COORDINATES: protein motif:HMM:NF013353.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleate cis-trans isomerase family protein"
                     /protein_id="HIR5099549.1"
                     /translation="MATWERKLGLIIPSWNTVMEYEFQRMAVPSMSVHSMRISHTADT
                     DENLTWMGTQVPAAAKLLAHAKVGVICYGCTAGGFLKGPGYDQQIIRDIETATGIPGA
                     ATAAAVVDALRALRATRVSVASPYEPWLNEKLQTYLKECGFEVLAIKGFGTQAHSSFT
                     PEQNAALVAEVDRSEAQAIFISCSNFRTLEIIEPLEQKLGKPVVTSNSASVWKMLRVI
                     GDGRRVSGGGQLLREA"
     gene            1666..>2498
                     /locus_tag="AC16G0_07760"
     CDS             1666..>2498
                     /locus_tag="AC16G0_07760"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR5099550.1"
                     /translation="MAKSEGAGGNSTATRWDEEYDLVVLGAGAGGMTAALVSAIEGMR
                     TLVIEQSNQVGGTTAFSSGSVWIPNNPEQRRSGITGDAEAARAYLNALVGGRADRALR
                     EAFLAAGPEMLEYLEKHTDVGFQMYRQAPDYRQELPGAAQGGRPLEPLPFDGRSLGKD
                     FDRVRWPIPELMLFGGMMVTRGEAARLLRIGRSWDSFALGARLVTRYLCDRLGYRRGT
                     RLVLGNALSARLFKNLLDRRVAIWFDGKTTRLLSEDGRACGLVVLRDAAEVRVRARRG
                     IV"
BASE COUNT          459 a          756 c          865 g          418 t
ORIGIN      
        1 ccaccctcgg cttcagcggt tttgaccagg gctatatcgc tgtcgaggcc gcccatcgat
       61 tcctgaaggg cgagaagctt ccgcagcaga ttattctgcc ggtgcccata gtggacaaga
      121 gcaatgcgga gccctacaac atcccgccgg aacagcggaa gccgccggtc tgggaccaag
      181 tcctggcaac acagaagtaa tgatccgaga aacgtctcag gggagggggc gcgtggcctc
      241 cccccctgat tggtatcttg gggaggatga ttccggtggt gagtgtggat aagcgcaatg
      301 ctcagactta caatgtccag cgaaaacagc ggaggccgcc ggtcagggac aagatcgttg
      361 cggcacaggg ctagagggga tcgcttgggc aatcgagaaa aggggaggga gagaactcgt
      421 cccctctccc tgtgccaggc ccggatacgg ctggcgtggt gagaggcgat cgaatgtgct
      481 ggttgacagg agggaatcca cacgtgaagg ccaagaatct caagcgggtc gtgcgggtgg
      541 agtcggttcc ccgctacgag gcccccgcgc cgtatcgccg attcgctcag gccctggtag
      601 atcaggagtc gttccccgag acctcgatgt gcgttgggta cttcacctac cccccggggg
      661 ccaagtcgca tacccaccac cacaccgcgg agaacgagat ctacttcgtc ctccaggggg
      721 agttgaccag cgtgcagaac ggcagcgcct ttcgggtgcc ccggcacggc ttggtcttca
      781 tcccgaaggg cgtctcgcat tacgccgcaa atcggacgcg acgccgctgc gtcttcctgg
      841 cggtccatgc acccgcggtg ccggacctcc tggagttcaa gcggtcctgg aagcggaccg
      901 cgaggcagag gagctaagcc atggcaacct gggagcggaa gttaggcctg atcatcccct
      961 catggaatac cgtcatggag tacgagtttc agcggatggc ggtacccagc atgtcggtcc
     1021 acagcatgcg tatctcccac acggccgata ccgacgagaa tctgacctgg atgggcaccc
     1081 aggtcccggc cgcggcgaag ctcctggccc atgccaaggt gggagtgatc tgctatgggt
     1141 gcacggccgg agggttcctc aaagggccgg gatacgacca gcagatcatc cgcgatatcg
     1201 agacggctac tgggatcccc ggggcggcca cggcggccgc ggtcgtggac gccttgcggg
     1261 cgctccgcgc cacacgggtg agcgtggcgt ctccctacga accctggttg aacgagaaac
     1321 tgcagaccta cctgaaggag tgcggcttcg aggtcctggc catcaaagga ttcggcaccc
     1381 aggcgcactc cagcttcacg cccgagcaga acgccgcgct ggtggccgaa gtggaccggt
     1441 ccgaggccca ggccatcttc atcagttgtt cgaattttcg cacgctggag atcatcgagc
     1501 cgctggagca gaagctgggg aagccggtcg tgaccagtaa ctccgcctcg gtgtggaaga
     1561 tgctgcgcgt gatcggcgat ggccgacgcg tttcgggggg cggccagctc ttgcgggagg
     1621 cctgaaggca gtatgccagc gacatggtaa aggggatcga tgcccatggc aaagagtgaa
     1681 ggcgccggcg ggaattcgac agcgacgcga tgggacgagg aatacgacct ggtcgtcctg
     1741 ggcgccgggg cgggtgggat gaccgcggcg ctcgtctcgg caatcgaggg catgcgtacc
     1801 ctcgtgatcg agcagagcaa tcaggtgggg ggcaccaccg ccttctcctc tggcagcgtc
     1861 tggattccca acaaccccga gcagcgccgt agcggcatca ccggggatgc cgaagccgcg
     1921 cgcgcgtacc tgaatgcgct ggtgggcggc cgggcggatc gcgccctgcg cgaggccttc
     1981 ctcgctgcgg ggcctgagat gctggagtac ctcgaaaagc acacggacgt gggcttccag
     2041 atgtatcggc aggctccgga ttatcgccag gagctaccgg gtgccgccca gggcggtcga
     2101 cccctggagc cgctgccgtt cgacgggcgc agcctgggca aggactttga tcgggtgcgc
     2161 tggcccatcc cggaactcat gctcttcggc gggatgatgg tgacccgcgg agaggcagcg
     2221 cgcctcctgc ggatcgggcg gtcgtgggat tcgttcgcgc tgggcgcccg gttggtgaca
     2281 cgctacctgt gtgatcgcct gggctaccgg cgggggacac gccttgtcct gggaaacgcg
     2341 ctctcggcga gactcttcaa gaatctgctc gaccgccgcg tggcgatctg gttcgacggc
     2401 aagacgacgc gacttctctc cgaggacgga cgagcctgcg gcctcgtcgt cctgcgcgat
     2461 gccgcggagg tgcgggtacg cgcgcgccga ggaatcgt
//
LOCUS       DBNGZC010000440         2403 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1110280, whole genome shotgun sequence.
ACCESSION   DBNGZC010000440 DBNGZC010000000
VERSION     DBNGZC010000440.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1110280"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..504
                     /locus_tag="AC16G0_07765"
     CDS             148..504
                     /locus_tag="AC16G0_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099551.1"
                     /translation="MAKTRAQPKSKSPFLGRWHIVSISNWDEEYFHEEVQAYIEFEPD
                     QRGEFQFGYVRGDIDYREGQRDGTPAVEWTWDGNDEMDPAQGRGWAVLEGDELHGKIF
                     FHLSDESDFVAERAGP"
     gene            complement(709..933)
                     /locus_tag="AC16G0_07770"
                     /pseudo
     CDS             complement(709..933)
                     /locus_tag="AC16G0_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012464.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(945..1265)
                     /locus_tag="AC16G0_07775"
     CDS             complement(945..1265)
                     /locus_tag="AC16G0_07775"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR5099552.1"
                     /translation="MESVRAPLTADESDRLSHACETPTEQRLIVWTLLDTGLRVSALC
                     SLTLKNILGQQRQLRFKGKGGPYGQKTKVCVIPMSHRVRALWEHHFALEKAFPVQAQR
                     DTRG"
     gene            complement(1300..2187)
                     /locus_tag="AC16G0_07780"
     CDS             complement(1300..2187)
                     /locus_tag="AC16G0_07780"
                     /inference="COORDINATES: protein motif:HMM:NF014646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HIR5099553.1"
                     /translation="MSTARVAEALGVGTTTVKRWVDQGILPAHKTPGGHRKILLSDVV
                     RVVREGDFPRLDLSRLGLPPEGQESPDPKSLSAALLAALKQGEAETLRSLLQGAHRSG
                     MAIETLADAVIAPAMRRLGHAWAAGRLEVWQEHQGTQLCAAAIYEIRATLQDPTDGKK
                     PLAIGGGPEGDPYLLANLLAEMALTGLGWKVVNLGQNTPLPSFRAALERTRPRLLWLS
                     ASHLVDPASFLEQYRAMYQEATRMGTAVAVGGQALTEEVRAQMPYTTFGDGLTHLVAL
                     ARSLHPGARRPRRGRPRGT"
BASE COUNT          417 a          789 c          803 g          394 t
ORIGIN      
        1 ccgacgatcg agcccgaccg gaccggggag gatcaggtcc catgaacgcc atcccgctgc
       61 tcgccctggc gacgtgtctc cccgccacga ccccaactcg gggagatgga cagatcccag
      121 ggcgggatga ggggaggcga atcgacgatg gccaagacca gagcccagcc gaagtcgaag
      181 tcgccgttcc tcggccgctg gcacatcgtc tcgatttcca actgggacga ggagtacttc
      241 cacgaggagg tccaagccta catcgagttc gagccggacc agcggggcga gtttcagttc
      301 ggctacgtcc gaggcgacat cgactaccgt gagggccagc gggacgggac gcccgccgtc
      361 gagtggacct gggacggcaa cgacgagatg gacccggccc aggggcgtgg ctgggccgtg
      421 ctggagggcg acgaactcca cggcaagatc ttcttccacc tgagcgacga gtcggacttc
      481 gtggccgaga gggccgggcc atgaggggcg tgagtagcag tgcgccgtgc aaaggcgcgg
      541 atcttcagcg ggtgcaactc ccgcccggat aactggtcgc tccatccggt agctatcgga
      601 gcggcggtgg aggcaacgaa gccgtcgaag ccttcgagac cacggaccgc ctcggcggtt
      661 cggcgagcag gcaggccgca acgcaagaga acgacagtct tgcacgacct tcatcagctt
      721 gttccagccc ggccggtcca tcgtcttgcc cgtaaactgg tcgcgatacc acctgacctc
      781 ggcgtcctga cccgcggccc agcgcttcaa gtcggactcc tggctggcgg tgtcctgcgc
      841 gcggctcgat accctagtgt agacggcgat gtgcttgctc atgaggcaag gatacgccgg
      901 ggcggtcgga gtgtcaagtg aatattctaa cattcacttg acaattaccc ccgggtgtca
      961 cgctgcgcct ggaccgggaa tgccttctcc agcgcgaagt ggtgctccca gagtgcccgc
     1021 acgcggtggg acatcggtat cacgcagacc ttggtcttct ggccataagg gccacccttg
     1081 cccttgaacc ggagctggcg ctgctgcccg aggatgttct tcaaggtgag gctgcacagc
     1141 gcgctgacgc ggagcccggt gtcgagcagg gtccagacga tgagccgctg ctcggtgggc
     1201 gtttcacagg cgtgggacag gcgatcggac tcgtcggcgg tgagcggcgc tcggacggat
     1261 tccatccgat ccgagaagac gacctctgac gccccacggt cacgtccccc gcggccggcc
     1321 gcgacgcggc cgtcgggccc ccggatggag ggaacgggcc aatgcgacga ggtgcgtcaa
     1381 cccatcgccg aaggtcgtgt agggcatctg ggcccgcacc tcttcggtca gggcctgccc
     1441 tccgacggca acggcggtcc ccatccgcgt agcctcttgg tacatcgcgc ggtattgctc
     1501 caggaacgac gccgggtcga cgaggtggct ggccgagagc cagagcaggc gcgggcgggt
     1561 ccgctccagg gccgcgcgga aggagggtaa tggcgtattc tgccccaggt tgaccacctt
     1621 ccatcccagg cccgtcagcg ccatctccgc gaggaggttc gccagcaggt acgggtctcc
     1681 ttccgggccg ccgccgatgg ccaggggctt cttgccatcg gtggggtcct ggagcgtggc
     1741 ccggatctcg tagatggcgg ccgcgcagag ttgggtgccc tggtgctcct gccacacctc
     1801 cagccggccc gcggcccacg cgtgcccgag ccgcctcatc gccggcgcga tgacggcatc
     1861 ggccaaggtc tcgatggcca tgcccgaccg atgcgccccc tggaggagcg aacgcaacgt
     1921 ttcggcctcg ccctgcttca gggcggccag cagcgccgcg ctcaacgact tagggtccgg
     1981 tgattcctgg ccctccgggg gaaggcccag gcggctgaga tccaggcgcg ggaagtcgcc
     2041 ttcgcggacg acccgcacga cgtcgctcag caggatcttg cgatggcccc ccggggtctt
     2101 gtgggccggc aagatcccct ggtccaccca gcgtttgacg gtcgtggtcc cgacccccag
     2161 ggcctcggcg acccgggctg tgctcacata cgggtttccg gcgtttggtc ccggctaggt
     2221 catctcagtg tccacagaga cgcgggcgcc ctcggcatgc acccgaccgg caagtgtcat
     2281 cgatcggcat cggaattgcc tagagccatt ctaccgtttt ggacgatatt ggctgagaaa
     2341 aaatcccaat aaatctgaaa atacccttta ctaatcgcaa tcgacttcca acaataaagc
     2401 agt
//
LOCUS       DBNGZC010000441         3727 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1897658, whole genome shotgun sequence.
ACCESSION   DBNGZC010000441 DBNGZC010000000
VERSION     DBNGZC010000441.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1897658"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..1402
                     /locus_tag="AC16G0_07785"
     CDS             314..1402
                     /locus_tag="AC16G0_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR5099554.1"
                     /translation="MQERVRVGAAQVSPHFFDKRKTLEKTLHTIQEAGQLGLDLLVFP
                     ETYFAAYPYWRGAVSVRRSTELVAERQRHAIRADGEEARAIADAAGRARVHCVVGCNE
                     LDDRKGSLTLYNTLLFAGRDGRILGRHRKLMPTHSERVYWGMGDASDIRTFDLDIGCV
                     GGLICYEHHMTLLRAAMAIKGEEIHCAVWPGWWKVERHLGGKTPEAGSETCDIAPAIR
                     EYAIENQAFVVSSSWFLTPDAVPADLRDELRYNLAVGGSCIVNPAGLYLKGPVFNQET
                     IVWTEIDEMDRRLAKAYFDCLGHYSRFDLLTLNIREDAWTPTGPRGAAPEVAGLPPEV
                     DLRRLANRYGLRIEELEAYLARGVAHAE"
     gene            1392..1646
                     /locus_tag="AC16G0_07790"
     CDS             1392..1646
                     /locus_tag="AC16G0_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099555.1"
                     /translation="MPSRPRLSPGAFLRRGVGAVVLVAVAGIFLVVINRAAPRSPLVI
                     LAACVLGVLAMKLVERRLKRWRQRWDAQEAARNRPAGPGR"
     gene            1655..2212
                     /locus_tag="AC16G0_07795"
     CDS             1655..2212
                     /locus_tag="AC16G0_07795"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HIR5099556.1"
                     /translation="MRSVPIGSARLELVEGDITQQDTEAIVNAANTSLLGGGGVDGAI
                     HRAGGPQILEACQKIGGCPTGEARITTGGRLKAKWVIHTVGPVYRDGKHREPELLASA
                     HRNSLKLASEEGIKSVAFPSISTGAYGYPMAEAARIGLQTVIEYLRKHPEITLVRFVL
                     FGQAALRTYDAVLAELSPGGDAGAS"
     gene            complement(2307..>3727)
                     /locus_tag="AC16G0_07800"
     CDS             complement(2307..>3727)
                     /locus_tag="AC16G0_07800"
                     /inference="COORDINATES: protein motif:HMM:NF014073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR5099557.1"
                     /translation="LFVGAVPGLTATMAAALLVPFTFFMDPLPALVAIVTMEAMAIFA
                     GDIPAALVRIPGTPSSAAYTDDSYELTKQGKAELVLGVDVITSAIGGLMGAVVLMTSA
                     PLLAEVALTFTSFEYFWLAALGLSATVMIASTNPLKAGLALLVGLFFSTVGLDITLGY
                     PRFTFGSTELLNGVDFIPAMIGLFGVSEILRNMGSSHLQYPITSVKAEKIFSGVAKTL
                     WRYKANMVRAGAIGTFIGILPGAGADIAAWIAYAAAKRFSKEPEKFGKGAIEPIVDAG
                     TANNACLAGDWVPALVFGIPGDSITAIVIGILFMKGLRPGPMIFQQQPELVYAVYISF
                     IIANILMVPFGYLAIKLSGTMLKVPRNILMPAILMFCIVGSFAINNSFFDIGVMLAMG
                     ILGYFFENNGIPVAPIVLGMVLGPIVEQNFMVSMIKTQWDLTQFFTRPTAAILAVLTI
                     LTWLAPFLPTLARRFRSQSAAA"
BASE COUNT          669 a         1171 c         1302 g          585 t
ORIGIN      
        1 gatcctgccc cggttccgga aagtgctggg ttccatgcgg ccggtgtcct ccaggaagat
       61 ggaggcccgg cctttttgtt tggcaaccaa tctcttctcc agagggcaag ccatttgagg
      121 gatggcccgc cggctctttt tgggctccgg ctattgaccc tgcacccgtc ggaggatacc
      181 ctgcgacaca gggggcagcc ggcagcaggc agagcgcaaa gagcaaggcg catggcgttg
      241 agtgcaaggc gaagaacgtt ggacgctctg ccccatgcgc agtgcgtcat gcgcccgaaa
      301 cacggaggga gccatgcagg agcgggttcg cgtcggtgcg gcgcaggtct caccccactt
      361 cttcgacaag cgcaagaccc tggagaagac cctccacacg atccaggagg ccgggcagct
      421 cggcctcgac ctcctggtct ttcccgagac ctacttcgca gcttatccgt actggcgtgg
      481 ggcggtgtcg gtccgccgct ccaccgagct cgtcgccgag aggcagcggc acgccatccg
      541 ggcggacggt gaggaggcgc gggccattgc cgatgcggcc ggccgtgccc gggtgcactg
      601 cgtcgtcgga tgcaacgagc tggacgaccg gaaggggagc ctcacgctct acaacaccct
      661 gctcttcgcg gggcgcgacg gccggatcct gggccgccat cggaagctca tgcccaccca
      721 ctcggagcgg gtgtactggg gtatggggga cgcctcggac atccggacct tcgatctgga
      781 catcggctgc gtgggcggcc tcatctgcta cgagcaccac atgacgctcc tgcgggcggc
      841 catggccatc aagggggagg agatccactg cgccgtctgg cccggctggt ggaaggtgga
      901 gaggcatctg ggcgggaaaa cgccggaggc cgggtcggag acctgcgaca tcgcgccggc
      961 catccgggag tatgccatcg agaaccaggc cttcgtggtc agctcgagct ggtttctgac
     1021 gcccgacgct gtgccggcgg atctccggga cgagttgcgc tacaacctgg cggtgggcgg
     1081 aagctgcatc gtgaacccgg cggggctgta cctgaaggga ccggtcttca accaggagac
     1141 catcgtctgg accgagatcg atgagatgga ccggaggctg gccaaggcct acttcgactg
     1201 cctcggccac tactcccggt tcgatctgtt gacgctgaac attcgagagg acgcctggac
     1261 gcccaccgga ccgcggggcg ccgcgccgga agtggcgggc ctccctcctg aggtggacct
     1321 ccgcagactg gcaaaccggt acggcttgcg catcgaggag ctggaggcct acctggctcg
     1381 cggtgtggcc catgccgagt agaccccgcc tgtctcccgg cgcgttcctg cgccgtgggg
     1441 tcggggcggt tgtcctggtg gcggtcgcag ggattttcct ggtggtcatt aaccgcgccg
     1501 ccccccgctc ccctctcgtc atcctggcgg cctgcgtcct cggcgtcctg gccatgaagc
     1561 tggtggagcg ccgtctgaag cgctggcgcc agcggtggga cgcgcaggag gcggcccgga
     1621 accggccggc cggcccgggg cggtaggagg tgtcatgcgc tcggtgccga tcggttcggc
     1681 ccgactggaa ctcgtggagg gggacatcac acagcaggac acggaggcca tcgtcaatgc
     1741 ggccaacacc agtctcctgg gtggcggcgg cgtggacggg gccattcacc gggcgggcgg
     1801 cccgcagatc ctggaggcgt gccagaagat cggcggctgc cctaccggcg aggcgcggat
     1861 caccacggga ggccgcctca aggccaagtg ggtgatccac accgtgggcc cggtgtatcg
     1921 tgacggcaaa caccgggagc ccgagctgct ggcgagcgcc catcgaaaca gcctcaagct
     1981 ggcgtccgag gagggcatca agagcgtggc cttcccctcg atcagcaccg gggcttacgg
     2041 ctatccgatg gcggaagccg cccgcatcgg tctccagact gtcatcgagt acctcaggaa
     2101 gcatccggag attaccctgg tgcggttcgt gctgttcggc caggcggccc ttcgtaccta
     2161 cgacgcggtc ctggccgaac tctcaccggg aggagatgcg ggcgcgtctt gagctgcgcc
     2221 acaccctcgc cggaaaagcg atacgcacgt gtcgggcctg acctcagggg tcgggcccga
     2281 cgtgttttac ggatacgggg ctgagatcag gcggcagcgc tctgggagcg gaaccggcgg
     2341 gccagagtgg gcaagaacgg cgcgagccag gtgaggatcg tcaggacggc caggatggcc
     2401 gcggtgggac gggtgaagaa ctgggtgagg tcccactggg tcttgatcat gctcaccatg
     2461 aagttctgct cgacgatggg acccaggacc atgcccagga cgatcggcgc aacggggatg
     2521 ccgttgttct cgaagaagta ccccaggatc cccatggcca gcatgacccc gatgtcgaag
     2581 aagctgttat tgatggcaaa agagccgacg atgcagaaca tcaggatggc cggcatgagg
     2641 atgttgcggg gcaccttgag catggtcccg ctcagcttga tggccagata cccgaagggc
     2701 accatcagga tgttggcgat gatgaaggag atgtacaccg catacaccag ctcgggctgc
     2761 tgctgaaaga tcatggggcc cgggcgcaag cccttcatga acaggatccc gatgacgatg
     2821 gccgtgatgg agtcaccggg aatgccgaag accagtgccg gcacccagtc gccggccagg
     2881 cacgcattgt tggccgtgcc ggcgtccacg atgggctcga tagccccctt tccgaacttc
     2941 tcgggctcct tggagaaccg cttggcggcc gcataggcga tccaggcggc gatgtcggca
     3001 cccgcacccg gcagaatccc gatgaaggtg ccgatcgccc ctgcccgtac catgttggcc
     3061 ttgtacctcc agagcgtctt ggccaccccg ctgaagatct tctccgcctt gacagacgtg
     3121 atggggtatt gcaggtggga gctgcccatg ttccgcagga tctccgacac gccgaagagg
     3181 ccgatcatgg ccgggatgaa gtccaccccg ttgagcagct cggtgctgcc gaaggtgaag
     3241 cgcgggtacc ccagggtgat gtccagaccg accgtggaga agaagagacc caccaagagc
     3301 gccaagccgg ccttcaaggg gttggtgctg gcgatcatga ccgtggccga caggcccagg
     3361 gcggccagcc agaagtactc gaaggaggtg aacgtcaggg ccacctcggc caggagcggg
     3421 gcggaggtca tgagaaccac cgcacccatc aggccgccga tggccgaggt gatcacgtcc
     3481 acccccagga ccagctccgc cttcccctgc ttggtcagct cgtaggaatc gtcggtgtag
     3541 gcggcggacg aaggggtccc cggaatccgt accagcgccg ccggaatgtc gccggcgaaa
     3601 atggccatgg cctccatggt cacgatggcg accagggccg gcagcgggtc catgaagaag
     3661 gtgaacggca cgagcagcgc cgcggccatg gtggccgtga gcccggggac ggccccgaca
     3721 aacaacc
//
LOCUS       DBNGZC010000442         1938 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_623533, whole genome shotgun sequence.
ACCESSION   DBNGZC010000442 DBNGZC010000000
VERSION     DBNGZC010000442.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_623533"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="AC16G0_07805"
     CDS             <1..663
                     /locus_tag="AC16G0_07805"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR5099558.1"
                     /translation="EVLEDKVYTRLKSLIIERRFEIGEKIQPDRLAKEMGVSRTPVLS
                     ALKRLAAEHLIECIPRRGMFVAHVSKRELVRLFEVREALECLSARLAATEVTDEQVDR
                     LGELFRGLDVSHTPEAVARYLERDRYFHWELVKISGNRYLMNAMEAVNVMTFTFQYDL
                     CRPIPEAIQEHESLLEALRKHDPDASESAMRSHISRTRALVMRAAIAEDQQGRGGQGP
                     ET"
     gene            1053..1277
                     /locus_tag="AC16G0_07810"
     CDS             1053..1277
                     /locus_tag="AC16G0_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099559.1"
                     /translation="MATVRKFMSALETARADGRASLGVDGKTVGTFVVERARPSLIRA
                     TATEAKEDKVRVAFATLGATPPKGALVHAS"
     gene            1267..>1938
                     /locus_tag="AC16G0_07815"
     CDS             1267..>1938
                     /locus_tag="AC16G0_07815"
                     /inference="COORDINATES: protein motif:HMM:NF024773.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR5099560.1"
                     /translation="MPPDTKSAIAAMLRPSSIAVIGATDRLYSGRLLNNLIHGGFKGE
                     LYPVNPKRDTVLGRRCYSSIRALPGPVDSAAILIPATGVLGALQECAEKGVKAAVIIS
                     AGFAEMGDLQGQQLQLELRRMARETGVRICGPNCLGVANVAEGIWTSANPSVEPDAVV
                     GPGAVALVSQSGATAYGPLMAVAKDRGIGFRYVVSTGNEADLDLTDFVEFLLDDPGVK
                     SIALMV"
BASE COUNT          381 a          588 c          637 g          332 t
ORIGIN      
        1 gaagtcctcg aggacaaggt ctatacccgc ctcaaatccc tgatcatcga gcggcgattt
       61 gagataggcg agaagattca gccggatcga ttggcgaagg aaatgggagt gagccggacg
      121 ccggtcctaa gcgcgctgaa gcgactggca gccgagcacc tcattgaatg cattccccgc
      181 cgtgggatgt ttgtagcgca cgtctccaaa cgcgaactag tccgcctgtt tgaggtccgg
      241 gaagcgctag aatgcctgtc cgcccgattg gcggctacgg aggtcactga cgagcaggtg
      301 gacaggctcg gcgaattgtt ccggggactg gacgtcagcc acacacctga agcggtcgcg
      361 cggtacctcg agcgggaccg gtatttccac tgggagttgg tcaagatctc cgggaaccgc
      421 tatctgatga acgccatgga ggcggtgaat gtgatgacct tcaccttcca gtacgatctt
      481 tgtcggccga tcccagaggc gatccaggaa cacgaaagcc tgctggaggc cttgcgcaag
      541 catgaccccg acgccagcga gtccgcaatg cggtcgcaca tcagccgaac gcgagcactc
      601 gtgatgcgcg cggcgattgc ggaagatcag caaggcaggg gagggcaggg accagaaacg
      661 taggatgcga cgcacaattg gccttggtcc ggaatttccc aacaccaagg caccaagaac
      721 gacaagaagg gaccaatgcc tcggagcatc ggccctcgga tcccgaagtc cacaggaggt
      781 acggctcagc tctctcgatt caatgcgaga atcccagcct agttgacgta cttggcgtca
      841 tggcggttca cgggtttcct ggaacagggt taggcgacgg ttcaccctcc gaccgcacag
      901 tccatgggag agtccagagg gggctcgcgg cccacagagg ggtggcggcc cgcctctctg
      961 ggtcgcctcc ggatcaccga catagctcgg tcccgaagac tttacgctcg acatcggggt
     1021 cgagcccagc agggacgggc gagagcagtt ccgtggccac cgtcaggaaa ttcatgtccg
     1081 ccctcgagac tgcccgggcc gatgggcggg cttcgcttgg cgtcgacggc aagacggtag
     1141 gcaccttcgt cgtggagcga gcgcggccta gcctcatccg ggcgaccgcc accgaagcca
     1201 aggaggacaa agtacgcgtc gcgttcgcca cgctcggggc aaccccaccg aaaggagccc
     1261 tggtccatgc ctcctgatac caaatcagcc attgccgcca tgttgcggcc gtcctcgatc
     1321 gccgtgatcg gagcgacgga tcgactctac agcggtcggc tcctcaacaa cctgatccat
     1381 ggcggattca agggggagct gtacccggtc aatcccaaac gggacacggt gctcgggcgg
     1441 cgctgttatt ccagcatccg cgccctgccc gggcccgtcg actcggcagc catcctcatc
     1501 cccgcaaccg gcgtccttgg ggcgctgcag gagtgtgcgg agaagggggt gaaggcggcc
     1561 gtcatcatct ccgcgggatt tgccgagatg ggcgacctgc aggggcaaca gttgcagttg
     1621 gagctgcggc gcatggcccg cgagaccggt gtgcggatct gcggtcccaa ctgcctgggc
     1681 gtcgccaacg ttgcggaggg catctggacc agtgccaacc ccagcgtgga gcccgatgcc
     1741 gtcgtcggcc ctggcgccgt ggccctggtg tctcagagcg gggcgaccgc atatgggcct
     1801 ctcatggccg tcgccaagga tcggggcatc gggttcaggt atgtggtgtc tacgggaaac
     1861 gaggcggatc tggacctcac agattttgtg gaattcctgc tggacgaccc aggggtcaag
     1921 tccatcgcct tgatggtc
//
LOCUS       DBNGZC010000443         1812 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_333005, whole genome shotgun sequence.
ACCESSION   DBNGZC010000443 DBNGZC010000000
VERSION     DBNGZC010000443.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_333005"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..1388)
                     /gene="murC"
                     /locus_tag="AC16G0_07820"
     CDS             complement(9..1388)
                     /gene="murC"
                     /locus_tag="AC16G0_07820"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR5099561.1"
                     /translation="MFKKIRRIHFVGIGGIGISGIAEVLHNLGYLVSGSDGRESENTR
                     HLVSLGIPVVIGHAAENIGQADVVVRSSAVPLDNSEVAAARGRLTPVIQRAEMLAELM
                     RMKYGVAVAGTHGKTTTTSMVAAVLAKGGLDPTIVIGGRLDALGTNAKLGRGDFLVAE
                     ADESDGSFLKLTPTIAVVTTLDAEHLDYYRDLAHIQDTFVEFINKVPFYGLAVLCLDQ
                     EHIQGLLPRVEKRFLSYGLHAQADLQAEDMSFSGMTSEFQVMAKRQPLGRIRLKVPGL
                     HNVYNALAATAVGLDLEIDFATIREALGEFAGVARRFQIKGEPGGILVVDDYAHHPTE
                     IQATLNAAKRGFGRRLIALFQPHRYTRTQALLKEFHTAFYQADRLFVTEIYPAGETPI
                     PGVHGGQIAEGVAEHGHRGVTYVPAKEDLVDAVLAEARPDDMVLTLGAGDIWRVGEEI
                     ARRLEAGTR"
     gene            complement(1408..>1812)
                     /locus_tag="AC16G0_07825"
     CDS             complement(1408..>1812)
                     /locus_tag="AC16G0_07825"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - obsolete lipid glycosylation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HIR5099562.1"
                     /translation="GGFQARAEAFFQDMATVYQAADFVIARAGASTVFELAAVGKPAL
                     LVPYPFAANDHQRLNAEAAVAAGAAWLVPDGYCDGQRIAAAIQAAREKPEQLRRMGEA
                     AQSLARPDAALRIVALLEQVAGAESKPESDRR"
BASE COUNT          286 a          646 c          593 g          287 t
ORIGIN      
        1 gcatgcgctc accgcgtccc cgcctcgagc cttcgcgcga tctcctcgcc gaccctccag
       61 atgtctccgg cccccaaggt cagcaccatg tcgtcagggc gcgcctctgc caggaccgca
      121 tccaccaggt cctccttcgc cgggacatag gtgacgcccc ggtggccgtg ctccgccacc
      181 ccctccgcga tttgcccccc gtggacgccg gggatcgggg tttcgccggc cgggtagatc
      241 tcggtcacga acagccggtc ggcctggtag aaggcggtgt ggaattcctt gagcaaggcc
      301 tgcgtccgcg tgtagcgatg cggctggaag agggcaatga gtcgccggcc aaacccccgc
      361 ttggccgcgt tcagggtggc ctggatctcc gtcgggtgat gggcgtagtc gtccaccacc
      421 aggatgccac ccggctcccc cttgatctgg aagcgccggg cgacacccgc gaactcgccc
      481 agggcctccc ggatcgtggc gaagtcgatc tccaggtcca gccccacggc cgtggccgcc
      541 aaggcgttgt acacgttgtg gagccccgga accttgagcc ggatgcgccc caggggctgg
      601 cgtttggcca tcacctggaa ctcggaggtc atcccggaga aggacatgtc ctccgcctgc
      661 agatccgcct gcgcatgcag cccgtagctc aggaaccgct tctccacacg cggcaggagg
      721 ccctggatgt gctcctgatc caggcacagg accgccaggc catagaaggg gaccttgttg
      781 atgaactcga cgaaggtgtc ctggatatgc gcgaggtcgc ggtagtagtc caggtgctcg
      841 gcatccagcg tggtcacgac ggcgatggtc ggcgtgagct tgaggaagga gccgtcgctc
      901 tcgtccgcct ccgccaccag aaaatcgccc cggccgagct tggcgttcgt ccccagggca
      961 tccaggcgcc ccccgatgac gatggtgggg tccaggccgc ccttggccag gacggccgcc
     1021 accatggacg tggtggtggt cttgccgtgg gtgccggcca cggccacgcc gtacttcatc
     1081 cgcatcagct ccgccagcat ctccgcacgc tggatgacgg gggtcaggcg cccgcgcgcc
     1141 gccgccacct ccgaattgtc cagcggcacg gcggaagagc ggaccacgac atcggcctgg
     1201 ccgatgttct ccgcggcgtg accgatgacc accgggatcc ccagggagac gaggtgccgc
     1261 gtgttctcgg attcccgacc gtccgagccg gagaccaggt acccgaggtt gtgcaggacc
     1321 tcggcgatgc cgctaatccc gatgccgccg atccccacga agtgaatccg acggatcttc
     1381 ttgaacatca cgcccccgga caaggaatca acgccggtcg ctctccggct tggactcggc
     1441 cccagcaacc tgctccaata gcgccacgat tcgcagggcg gcgtccggcc gtgccagcga
     1501 ctgggcggcc tcccccatgc ggcgaagctg ctccggcttt tctcgggcgg cctggatggc
     1561 ggccgcgatc ctttggccgt cacagtagcc gtccggaacc agccaggccg cgccggcggc
     1621 cacggccgcc tcggcattca atcgctggtg atcgttggcc gcgaagggat agggaaccaa
     1681 gagcgcgggc ttccccacgg ccgccagctc gaagacggtg ctggcgccgg cccgcgcgat
     1741 gacgaaatcc gcggcttggt agaccgtggc catgtcctgg aagaaggcct ccgcccgcgc
     1801 ctgaaatccg cc
//
LOCUS       DBNGZC010000444         3046 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1211480, whole genome shotgun sequence.
ACCESSION   DBNGZC010000444 DBNGZC010000000
VERSION     DBNGZC010000444.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1211480"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..860)
                     /locus_tag="AC16G0_07830"
     CDS             complement(168..860)
                     /locus_tag="AC16G0_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HIR5099563.1"
                     /translation="MRPYDLHFFPYPPLYLLALLLILGVLLTLLQLGQIRYVYERLGL
                     NRHAAVAILFAALLGSGMNIPLAELPAQRVVQPAIVDFFGMQYVIPQVVERNSTVIAV
                     NVGGAVIPVLLAIYFMSRHGVRGRMLITLGVATLVTHQLAQPVPGVGIALPPLLPSLV
                     TAGMAILVDRQEAPRTAFIAGTLGTLIGADLLNLGVVQDLGAPVVSIGGAGTFDGVFL
                     AGILAVLLAGLI"
     gene            complement(889..1293)
                     /locus_tag="AC16G0_07835"
     CDS             complement(889..1293)
                     /locus_tag="AC16G0_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HIR5099564.1"
                     /translation="MEPLTWMELTISYVLRVGVLLSAAIILAGVLLFAIRQDTGYAKV
                     LPNHLQALLSFHQTSGPGFFPTSLEEVGRGAVAGRPYAIICLGILLLIATPVVRVALS
                     VLFFLVQGDWLYVGITLFVLGVLLLSMFAGIG"
     gene            complement(1326..2174)
                     /locus_tag="AC16G0_07840"
     CDS             complement(1326..2174)
                     /locus_tag="AC16G0_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR5099565.1"
                     /translation="MEPLTFFLILFGASMVAGALGALLGLGGGVIIVPILTLMAGLNI
                     HYAIGASIVSVIATSSGAAATYVRDKITNLRLGMFLEIATTTGAVTGALLAGLLPGRI
                     LSVVFGLMLVHAAWSMYRSGHEDVPVGVVGSPLATRLRLHGAYFDPALGRRVDYQVRG
                     VPQGFSVMWLAGLISGLLGIGSGVFKVIAMDIMMRAPIKVSTTTSNFMIGVTAAASAG
                     IYFIRGDIHPFVAAPVALGILTGTIIGTHLLLRLRGAAVRGVFLVVLIVIAIQMILRG
                     IGGSVR"
     gene            complement(2196..3041)
                     /locus_tag="AC16G0_07845"
     CDS             complement(2196..3041)
                     /locus_tag="AC16G0_07845"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0030497 - fatty acid elongation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HIR5099566.1"
                     /translation="MNRIPAGRRPELNEQEPTLHGWGLILGASSGFGEATALALAQHG
                     LHIFGVHLDRKATLANVDRIVGEIKALGSEAIFFNVNAADPEKRQEVLDQVGKTLADR
                     GEPGSLTVLLHSLAFGALKPFITEDPKEALAQSQMEMTLDVMAHSLVYWVQDVIRHGL
                     MGAGGKIFAMTSSGGNHVWPTYGAVSAAKAALEAHIRQLAYELAPRKITANAIRAGVT
                     DTPALRKIPGNDKLIQGALGKNPHGRLTTTSDVASAIAALCVDGTQWISGNVIGVDGG
                     EDLTG"
BASE COUNT          604 a          981 c         1007 g          454 t
ORIGIN      
        1 accgttggca acctcgtagc tgccgcagcc gaccacctcg ccgctgggag aacgcccagc
       61 aggccaaccg ctatgtgccc aacgcccacg gcagcgccga ctcggtcatt gtcatcgaat
      121 gaccggcacg caccggaccc agcgcagccg gtcacgcagg gcagggctta gatgagcccg
      181 gcgagcagga ccgcgaggat accggcgagg aagacgccgt cgaaggtgcc ggcgccgccg
      241 atggagacca ccggggcgcc caggtcctgc acgactccca ggttcagcag gtcggccccg
      301 atcaaggtgc caagcgttcc ggcgatgaag gccgttcggg gtgcctcctg ccggtcaacc
      361 aggatggcca tcccggcagt cacgagtgac ggcaagagcg gggggagggc gatccccacg
      421 cccggcaccg gttgcgccaa ttgatgcgtg accagggtgg ccacgcccaa ggtgatcagc
      481 atgcgacccc tgaccccgtg tcgggacatg aagtagatcg ccagcaggac aggaatgacg
      541 gcacccccga cgttgaccgc aatcacggtg ctgttccgct ccaccacctg aggtatgacg
      601 tactgcatac cgaagaagtc gacgatcgcc ggctggacca cacgctgcgc cggcagctcc
      661 gccagcggaa tgttcatccc gctgccgaga agggcggcga ataggatggc gacggcggcg
      721 tgccgattca gtccgagacg ctcgtagacg tagcggatct ggccgagttg caacagggtg
      781 aggagcacgc ccaggatcag caggagggcc aagaggtaga gcgggggata gggaaagaag
      841 tgcagatcgt agggtcgcat ggtgcccccg acagagaggt ctggggtgct acccgatacc
      901 ggcgaacatg ctcaggagca agacccccag gacaaacagg gtgatcccca cgtagagcca
      961 gtctccctgg accaagaaga agagaaccga cagggccacc cgcacgacgg gcgtcgcgat
     1021 cagcaagaga atccccagac agatgatggc atacggtctt cccgcgaccg cgcctcgccc
     1081 aacctcctcg agcgaggtgg ggaagaaacc gggcccgctg gtttgatgga aagagagcag
     1141 agcctggaga tgattgggga ggaccttggc gtatccggtg tcctgcctga tggcaaacag
     1201 tagcacgcct gccaggatga tcgccgcact caagaggacc ccgacccgga ggacgtagct
     1261 gatcgtcagc tccatccaag tgaggggctc catgccctca ggcggactct ccggcttccc
     1321 tggggtcatc ggacactccc tccgatcccc cgcaggatca tctggatcgc gatcacgatc
     1381 agcaccacca agaagacccc acgaacggcg gcgccgcgca agcggagaag gaggtgggtc
     1441 ccgatgattg tacccgtcag aatccccaga gccaccgggg cagcgacgaa aggatgaatg
     1501 tccccccgga tgaaatagat cccggcgctg gcagccgcag tcacgccgat catgaagttg
     1561 ctggtggtcg tggacacctt gatgggagcg cgcatcatga tgtccatggc gatgaccttg
     1621 aacacgccgc tgcctatccc caaaaggccc gagatgagcc ccgccagcca catcacgctg
     1681 aaaccctggg ggacccccct gacctggtag tctacacgcc ggcccagggc cggatcgaaa
     1741 taggccccgt ggagccgcag acgcgtggcc agaggactcc ccaccacccc caccgggacg
     1801 tcttcatgac ccgagcgata catgctccag gccgcgtgca ccagcatgag gccgaagacc
     1861 accgagagga tccgcccggg gagcaagccg gccaggaggg cccccgtcac ggcccccgtg
     1921 gtggtggcga tctcaaggaa catcccgagc cgcaggttgg tgatcttgtc tcggacgtag
     1981 gtggcggcgg cgccgctgga ggtggcgatg acggagacga tgctggcccc gatggcgtaa
     2041 tggatgttga ggccggccat gagggtgagg atggggacga tgatgacccc gcccccgagt
     2101 cccaggaggg cccccagggc gcctgccacc atggaggcgc cgaagagaat caggaagaac
     2161 gtcagcggtt ccacgcgcca acccgggtca cgactttacc ccgtcaggtc ctcgcctccg
     2221 tccacgccga tgacgttccc tgagatccac tgggtgccat ccacgcacag cgcggcgatg
     2281 gcggacgcga cgtcggaagt tgtggtgagt ctcccgtgcg gattcttgcc cagggcaccc
     2341 tggatcagct tgtcgttgcc gggaatcttc cgcagggcgg gggtatcggt gacaccggcg
     2401 cggatggcat tggcggtgat cttccgtggc gcgagttcgt atgccaactg acggatgtgg
     2461 gcctccagtg ccgccttggc cgcggacacg gccccgtagg tgggccagac gtggttcccg
     2521 ccggagctgg tcatggcaaa gatcttgccg ccggcgccca tcagcccgtg ccggatcaca
     2581 tcctggaccc agtacaccag gctgtgggcc atgacgtcca gggtcatttc catctgggat
     2641 tgggcaagag cctccttggg gtcctcggtg atgaaaggct tcagggcgcc aaaggcgagc
     2701 gaatgcagga gcaccgtcag cgaaccgggc tcgccgcggt ccgccagggt cttgcccacc
     2761 tggtccagga cctcttggcg tttctccgga tccgcggcgt tcacgttgaa gaagatcgcc
     2821 tcgctgccca gggccttgat ctcgcccacg atccggtcaa cgttggccag ggttgccttg
     2881 cggtccagat ggacaccgaa gatgtgcaac ccatgctgcg ccagggccaa ggcagtggcc
     2941 tcgccgaacc cgctggaggc gccgaggatc aggccccatc catgaagcgt cggctcctgt
     3001 tcgttcaatt cgggtctcct tccggctggg atgcggttca catggc
//
LOCUS       DBNGZC010000445         2529 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1993211, whole genome shotgun sequence.
ACCESSION   DBNGZC010000445 DBNGZC010000000
VERSION     DBNGZC010000445.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1993211"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="AC16G0_07850"
     CDS             <1..661
                     /locus_tag="AC16G0_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR5099567.1"
                     /translation="PGEVSLAHHGVLFLDELPEFKRHVLEVLRQPLEDGQVTIARAQS
                     SLTYPARFMLAAAMNPCPCGYATDPTKECSCTPLQIQRYLARISGPLLDRIDIHIDVP
                     PLKNRELTGEPSGEPSATIRERVRTARRIQQDRFRRSKIYCNAHMSTRQIRKHCQADG
                     EARTLLETAMERLGLSARAYDRILKVARTIADLESLETIQPEHIAEAIQYRSLDRGPR
                     R"
     gene            1456..>2529
                     /locus_tag="AC16G0_07855"
     CDS             1456..>2529
                     /locus_tag="AC16G0_07855"
                     /inference="COORDINATES: protein motif:HMM:NF049980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate MFS transporter"
                     /protein_id="HIR5099568.1"
                     /translation="MSAEGYINRWWRVVGALLMNLSLGSLYAWSIFVAPLEKEFGWNR
                     AQTSWIFTIAVFVFGMSFVIAGRLQDKLGPFKISVVGSIGLSLGFILASFTNSLGWIF
                     FTFGGIMGFGNGFGYATPIPVLSKWFPDRRGLAVGLAVAGYGGGSAILAFIGPPMLSG
                     LGWRGTFLWLGIGFFAATIIGAFLLQNPPVGWKPAGWVPPPPTAKVSATTYDYTPSEM
                     VRTPQFPLMWIGYCLGTSAGLMTISQLIPFAKSVGLPATAATLTLLIGAIGNAGGRIF
                     SGWLSDAMGRLQTLRTMIAVSALAMVVLAHSASVGVVYVVLFLVYYCYGTCLSVFPST
                     TADFFGTKNLGVNYGWIFTAWGVA"
BASE COUNT          453 a          774 c          796 g          506 t
ORIGIN      
        1 gcctggcgag gtgagcctgg cgcatcacgg cgtcctcttc ttggacgagc tcccggaatt
       61 caagcgtcac gtcctggagg tcctgcgcca gcccctggag gacggtcagg ttaccatcgc
      121 gcgggcccaa tcctcgctca cctatccggc gcggttcatg ctggccgccg ccatgaatcc
      181 ctgcccctgc ggctacgcca cggacccgac gaaggagtgc tcctgcaccc cgctccagat
      241 ccagcgctac ctcgcgcgta tctcgggacc gctcctggat cgcatcgaca ttcacattga
      301 tgtcccgccg ctgaagaacc gagaattgac tggagagccg tcgggagagc cgtccgccac
      361 catccgcgag cgggtgcgca ccgcgcgccg gatccagcag gaccgcttcc ggcgcagcaa
      421 gatctactgc aacgcccaca tgagcacccg acagatccgc aagcactgcc aggcggacgg
      481 cgaggctcgc acacttctgg agactgccat ggagcgcctc ggcctctcgg cccgggcgta
      541 cgatcgaatc ctgaaggtcg cccggacgat cgccgacctc gagagcctcg agacgattca
      601 acccgagcac attgccgagg ccatccagta tcggtccctg gatcgcggcc cccggcggta
      661 gcctccgatg gaagggagcg gggaaggccc gcccgttctc tccgtgtggc cgctgggtta
      721 gttccccggt ggaaccggct tgcccgcaaa catggggacc tgcctgagcc ccctgacatg
      781 ctggagcatg gttcgcaggg ggcggcccac atggccaccg accgtttacg gctccatcca
      841 aattacaggc gccggtgcga tcaagacgca cgcctgcaga ccacccgtca aacctactcc
      901 gccagtggtc agcgcaatgc ctcgcctatg gctaggaacc tccaagactt ttgtataaat
      961 gaacagttac aggggataaa ccgggccaaa accgtttcct ggaacttttc ccttgacagt
     1021 attcaacaca tgttttagtt ttcgcatact acgccttcca agaagcttcc cgtagcacgc
     1081 attgagttat ccgcccccta aggtaacgat agaagatcct gccttgtgta gtacttagtt
     1141 ggcctcgacc aaagggaggg ggatcccggc ggcagccaac caaaacgcag tttcacaatt
     1201 tggaggtggt gtggagccaa tgcggagtag aacgatctac gcggtgagcc agtgtgttca
     1261 gatttgaggc agtgaggttc tcataggcag gttctagacc acgaggggag ggacgtcggc
     1321 tgaaggaaat agaccagggt atgagtgcac ggcggcagga cgattgggca tcatatgagg
     1381 taatacgcgt gaggatgccg tcgtgaggtt taagtcctgg gggaggcggc ttgggggccg
     1441 caaaggagga aagggatgag tgcagagggt tatatcaatc gatggtggcg ggtggtgggc
     1501 gctttattga tgaacttgtc gctggggtcg ctgtacgcct ggagtatctt cgtggcgccc
     1561 ctggagaaag agttcgggtg gaaccgagcg cagacctcct ggatcttcac catcgcggtg
     1621 ttcgtcttcg ggatgtcctt cgtgattgcg ggccgcctcc aggataagct gggtcccttc
     1681 aagatctccg tcgtgggatc tatcggcctc agccttgggt tcatcctggc cagcttcacc
     1741 aacagtctgg ggtggatctt cttcaccttc ggaggcatca tgggcttcgg gaacggtttc
     1801 gggtatgcca ccccgattcc cgtcctctcc aagtggtttc cggatcgtcg cggcctggcg
     1861 gtcggcctcg ccgtggccgg atatggcggc ggttcggcca tcctggcctt catcggcccg
     1921 cccatgctga gcggcctcgg gtggcgaggg accttcctct ggctggggat cggattcttt
     1981 gcggccacca tcatcggggc cttcctgctg cagaatcctc cggtcgggtg gaagccggcg
     2041 ggctgggttc cgcccccgcc gacggccaag gtctctgcga caacgtatga ctacacacct
     2101 tccgagatgg tccgaacgcc ccagttcccc ctgatgtgga tcggttactg cctgggcact
     2161 tcggccgggc tgatgaccat cagccagctc attcccttcg cgaagagcgt gggactgccg
     2221 gccaccgcgg ccaccttgac gctgctgatc ggggcgatcg ggaacgcggg gggtcggata
     2281 ttctcgggct ggctgtcgga tgcgatgggc cgactgcaga cgttgcgcac catgatcgcg
     2341 gtatccgcgc tcgccatggt ggtcctggca cacagcgcca gcgtcggcgt cgtgtatgtg
     2401 gtactcttcc tggtgtacta ctgctacggg acgtgtctgt cggtattccc gtccaccacg
     2461 gcagacttct tcggcaccaa gaacctgggc gtgaactacg gctggatctt cacagcgtgg
     2521 ggcgtggct
//
LOCUS       DBNGZC010000446         3312 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2678256, whole genome shotgun sequence.
ACCESSION   DBNGZC010000446 DBNGZC010000000
VERSION     DBNGZC010000446.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2678256"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="AC16G0_07860"
     CDS             <1..474
                     /locus_tag="AC16G0_07860"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017364447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HIR5099569.1"
                     /translation="GTKIEAYEMKMASLVILIGPAIMLIGTAVAVVTPAAVASMSNPG
                     PHGFSEVLYAFSEGAANNGSAFAGLNANTLFYNIGIGLAVLFGRFWMLIPPLAIAGSL
                     ARKKIVPASAGTLPTHTPLFVALLVGTIILVGALTFFPALALGPIVEHLQMLAMK"
     gene            487..2559
                     /gene="kdpB"
                     /locus_tag="AC16G0_07865"
     CDS             487..2559
                     /gene="kdpB"
                     /locus_tag="AC16G0_07865"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015600820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HIR5099570.1"
                     /translation="MTTQAKARPLFDPAIVWQATIDSFRKLTPQRQIRNPVMFVVYVG
                     SILTTLLFFHALVGQGEAPAGFILAVSVWLWFTVLFANFAEAMAEGRGKAQAASLRKA
                     RRDIQAKRLTRPEREAQVTVVPGTSLRKDDVVLVEAGDFIPADGEVIDGIASVNESAI
                     TGESAPVIRESGGDRSSVTGGTQVLSDWLIVQVTANPGEAFLDRMIALVEGAKRQKTP
                     NEIALDVLLAGLTIVFLLACATLLPYSLYSVKAAGHGTPITVTVLVALLVCLIPTTIG
                     GLLSAIGIAGMDRMIQANVIAMSGRAVEAAGDVDVLLLDKTWTITLGNRQATAFLPAP
                     NVPEKVLADAAQMSSLPDETPEGRSIVVLAKEKYGLRERDLRAMGATFVPFTAQTRMS
                     GVNLNGRQIRKGAADAIEAYVKDLGGTVPPHVQMTVEKIAKDGATPLVVAEGAKVLGV
                     IQLKDIVKGGIKERFAELRRMGIKTVMITGDNRLTAAAIAAEAGVDDFLAQATPEAKL
                     KLIRAIQAEGRLVAMTGDGTNDAPALAQADVAVAMNTGTQAAKEAGNMVDLDSNPTKL
                     MEVVEVGKQLLMTRGALTTFSIANDVAKYFAIIPAAFATTYPALKALDIMHLATPESA
                     IMSAVIFNALIIIALVPLALRGVKYRALGAAAVLRQYLWIYGVGGIIIPFVGIKLIDM
                     ILVALRLV"
     gene            2571..3146
                     /gene="kdpC"
                     /locus_tag="AC16G0_07870"
     CDS             2571..3146
                     /gene="kdpC"
                     /locus_tag="AC16G0_07870"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HIR5099571.1"
                     /translation="MWKHLRPAIVSLVALTILTGLVYPMVVTGIAQVLFPRQANGSLI
                     MKDGKAVGSELIGQPFDEAKYFWGRPSATSPFPNNAGSSSGSNLGPTNPDLQKAVQGR
                     IEALRAADPGNTAPVPVDLVTASGSGLDPDISPAAAQYQVRRVAKARGLDESVVRNLV
                     EQYTQRRQFGFLGEPRVNVLRLNLALGETKK"
BASE COUNT          586 a         1061 c         1103 g          562 t
ORIGIN      
        1 ggcacgaaga tcgaggccta cgagatgaag atggcgtcgc tcgtcatcct gatcgggccg
       61 gccatcatgc tgatcgggac agccgtcgcc gtggtcacgc cggcagccgt ggcctcgatg
      121 agcaacccgg gcccgcacgg cttcagcgaa gtcctctacg ccttctccga aggcgcggcg
      181 aacaacggca gcgcctttgc cgggctgaac gccaacaccc tcttctataa catcgggatc
      241 ggcctggcag tgctcttcgg tcgcttctgg atgctgatcc cgcctctggc catcgcggga
      301 tccctggcgc ggaagaagat cgtaccggcg agcgcgggga cgctccctac gcacacaccc
      361 ctgttcgtgg cccttctggt gggtacgata atccttgtgg gggcgctcac cttcttcccg
      421 gcgttggccc tcggccccat cgttgagcat ctccagatgc tcgccatgaa gtgaggtgag
      481 actcccatga caacgcaagc gaaggcccgt ccgctgttcg atcctgcgat cgtgtggcag
      541 gcgacgatcg attcgttccg gaagctgaca ccgcaacgcc agatccggaa cccggtcatg
      601 ttcgtggtgt acgtggggtc catcctcacg acgcttctat tcttccatgc cctggtgggc
      661 caaggggagg cgccggctgg gttcatcctg gccgtgtcgg tgtggctctg gttcacggtg
      721 cttttcgcca acttcgccga ggcgatggcg gaaggccgcg gcaaggccca ggcggcctcc
      781 ctccgaaagg cccgccggga cattcaggct aagcgcctga cccggccaga gcgcgaggcg
      841 caggtgacgg tagtcccggg cactagcctg cggaaagacg acgtcgtgct ggtcgaggcc
      901 ggagacttca tcccggccga tggcgaggtg atcgacggca tcgcctcggt gaacgagagc
      961 gccatcaccg gcgagagcgc gcccgtgatc cgcgagagcg gcggcgaccg cagctcggtg
     1021 acgggcggga cgcaggtact ctccgactgg ctgatcgtgc aggtgacggc aaatcccggc
     1081 gaggcgttcc tggatcgcat gatcgccctg gtcgaggggg ctaagcgtca gaagacaccc
     1141 aacgagatcg ccctggacgt tctcctggcc ggtctgacca tcgtgtttct gctggcgtgc
     1201 gccacgctgt tgccctactc gctctacagc gtgaaggcgg cgggccacgg gaccccaatc
     1261 acagtcaccg tgttggtcgc cctgctggtt tgcctgatcc cgaccaccat cggtggcctc
     1321 ctctccgcca tcggcatcgc gggcatggac cggatgatcc aggccaacgt gatcgccatg
     1381 tcggggcgcg cggtggaggc ggcgggtgat gtggatgtgc tcctcttgga caagacgtgg
     1441 acgatcaccc tggggaaccg gcaggcgact gccttcctcc cagcgcccaa tgtcccggag
     1501 aaagtcctgg ccgacgcggc tcagatgtcc tccctgccgg atgagacacc ggagggtcgc
     1561 agcatcgtcg ttctcgccaa ggagaagtac ggcctgcgag agcgggacct ccgtgccatg
     1621 ggcgccacgt tcgtcccctt caccgcccag acccggatga gcggggtcaa cctgaacggc
     1681 cgccagatcc ggaagggcgc cgcggatgcc atcgaggcat acgtcaagga tctgggcggc
     1741 acagttcccc cgcatgtcca gatgacggtg gaaaagatcg ccaaggacgg agcgaccccc
     1801 ctggttgtgg cagagggggc gaaggtcctc ggggtgatcc agctcaaaga tatcgtgaag
     1861 ggcggcatca aggagcggtt cgcggagctg cgccgcatgg gcatcaagac cgtcatgatc
     1921 accggagaca accggctgac ggcggcggcc atcgccgccg aggccggcgt ggacgacttc
     1981 ctggcccagg ccacacccga ggcgaagctc aagctgatcc gtgccattca agcggagggc
     2041 cggctggtcg cgatgaccgg ggacggcacc aacgatgccc cggcgctggc ccaggcggat
     2101 gtcgccgttg ccatgaacac cggcacccag gcggccaagg aagccggcaa catggtggac
     2161 ctcgattcca acccgaccaa gctgatggag gtggtcgagg tcggcaagca gctcctgatg
     2221 acccgcgggg cgctcacgac cttcagcatc gccaatgacg tggccaagta cttcgcgatc
     2281 atcccggcgg cgttcgcgac cacctatccc gccctcaagg cgctggacat catgcacctg
     2341 gccacgccgg agagcgcgat catgtcggcg gtcatcttca acgcgctgat catcatcgcg
     2401 ctggttcccc tggcgctccg tggggtgaag taccgggccc tcggggccgc tgcggtgctc
     2461 cggcagtacc tctggatcta cggggtgggc gggatcatca tccccttcgt cggcatcaag
     2521 ctgattgaca tgatcctggt cgctttgcga ctggtgtaag gagaacgccc atgtggaagc
     2581 atcttcgacc ggcgatcgta tctctggtgg ccttgacgat cctcacgggt ttagtctacc
     2641 cgatggtggt gacggggatt gcgcaagtac tcttcccgcg gcaagcgaac gggagcctga
     2701 tcatgaagga tgggaaggct gtgggctcgg agttgatcgg ccagcccttc gatgaggcca
     2761 agtacttctg ggggcggcct tcggcgacct cgcccttccc gaataacgcc ggatcgtctt
     2821 cgggctcgaa tctcggcccc accaaccccg acctccagaa ggcggtgcag gggcggatcg
     2881 aggctctccg ggcggccgat cccggaaaca cggcgcccgt cccggtggac ctggtcacgg
     2941 cgtccgggag cggtctcgat cctgacatca gtccagccgc agcccagtac caagtccgac
     3001 gggtggccaa agcccgggga ctggatgaaa gcgtggtgcg gaacctagtg gagcagtata
     3061 ctcaacgtcg gcaattcggg ttcctgggcg agccaagggt gaacgtgctc cggctcaacc
     3121 tggccctggg cgagacgaag aagtagcctt caaccgtccg aatcaactca ggagatcctc
     3181 gccgtgaacg atcgtcggcc cgacccggat gaactgctgg cccgggtcaa ggccacggag
     3241 gctcgccaga cacgggggaa gctcaagatc ttcctcgggg cggcagcggg ggtggggaag
     3301 acctttgcca tg
//
LOCUS       DBNGZC010000447         1685 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_232818, whole genome shotgun sequence.
ACCESSION   DBNGZC010000447 DBNGZC010000000
VERSION     DBNGZC010000447.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_232818"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1473)
                     /locus_tag="AC16G0_07875"
     CDS             complement(154..1473)
                     /locus_tag="AC16G0_07875"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5099572.1"
                     /translation="MGWIPAGRKKPASMKDAFDLILQNCRLPDGSLVDIGCCNGRITE
                     IGTLADHAVDRVVACEGCLVTPGLVEAHIHLDKVLLSERAPSTEGTLAEAIRVTGEAK
                     RRFSVEDIRSRARAVLDMAVRSGTTAMRSHVEVDPIVGLKGLEALLPLKKEYAPALDL
                     QLCAFAQEGILQAPGTEALLADALRQGADLVGGCPYNDTDAHAQIDIIFRLAKEFGVD
                     VDFHTDFFDEAEHLHVCYIARQTVRQGWQGRVAVGHLTELAALPPEEQDPIIASLREA
                     GVAVIVLPATDLYLMGRRDLKNVRRGLAPVKRLLAGGVTVAAATNNVRNAFTPVGNAS
                     LPLMGYLLAVGAHMGTSREIQQALAMLTENPARILRIADYGLRVGARADLVLWEVKRQ
                     DEIIAALANPRLVVKNGRVTVEHERTVREPWRAAPPSEAKASQRPGS"
BASE COUNT          271 a          609 c          515 g          290 t
ORIGIN      
        1 caggctgtcc tggcaaatat cggtcttgac caccccgact tctgaccaca cttccggccg
       61 agtctaccgg tgtaaagtcc gcatcacaag agccggcgac aatccccgtg tgatggccac
      121 ctgctcaccc ccaccccttc gtatccattg gccttagctg ccaggtctct gcgaggcctt
      181 ggcctccgac ggaggggccg cccgccaggg ctcccgcacg gtccgctcgt gctccaccgt
      241 gactcgacca ttcttcacca ccaggcgggg gttggccagg gcggcgatga tctcgtcttg
      301 acgcttcacc tcccagagga cgaggtccgc tcgggccccg acccgcagtc cgtaatcggc
      361 gatcctcagg atgcgggccg gattctccgt caacatcgcc agggcctgct ggatctcccg
      421 cgaggtcccc atgtgggcac ccaccgccag caggtacccc atgaggggga ggctggcgtt
      481 gcccacaggc gtgaaggcgt tccgcacgtt gttcgtggcg gcggccaccg tgacgccccc
      541 ggccaggagg cgcttcacag gggcgagccc gcgccggaca ttcttgaggt cgcgccgccc
      601 catcaggtag aggtcggtgg cgggaagcac gatcacggca acgcccgcct cgcgcagact
      661 ggcgatgatc ggatcctgct cctccggggg cagagcggcc agctccgtga ggtggcccac
      721 ggccacccga ccctgccagc cctgccgcac ggtctgccgg gcgatatagc acacatgcag
      781 gtgctcggcc tcgtcgaaga aatccgtgtg gaagtccacg tccacgccga actccttcgc
      841 cagccggaag atgatgtcga tttgggcgtg ggcatcggtg tcgttatagg ggcatccccc
      901 cacgaggtct gcgccctgtc gtagcgcgtc ggcgagcagc gcctcggtgc ccggggcctg
      961 gaggatcccc tcctgggcaa aggcgcagag ctgaagatcc agcgccgggg cgtattcctt
     1021 cttgagcggg agaagggcct ccagcccctt gaggccgacg atggggtcca cctccacgtg
     1081 gctgcgcatt gccgtggtgc cagaacgcac agccatgtcc aggacagccc gcgcccggga
     1141 ccggatgtcc tccacgctga agcggcgctt ggcctcccct gtgacccgga tcgcctcggc
     1201 aagcgtcccc tcggtgctgg gggcgcgctc gctgaggagc accttgtcca gatggatatg
     1261 tgcctcgacg agcccggggg tcaccaagca gccttcgcat gcgaccaccc ggtcgaccgc
     1321 atggtccgcg agggtgccaa tctctgtgat gcggccgtta cagcagccaa tatccaccag
     1381 gcttccgtcc ggcaaccgac agttctgcaa gatcagatcg aaggcgtctt tcatgctggc
     1441 cggcttcttc cttcctgcgg gaatccaacc catccagtcc cccacctgtc tctcgtggat
     1501 tcaggcttca ggcttcgctg gaccatgtcg gacccccgac ctggatgacg aaccatgcat
     1561 cgcccacggc tcaatcctcc cgcacgatgt gtgtccccgc cttccccgcc accgcttcgc
     1621 ccacgcgatc cagagaggtg atgatggcca cccggccgcc gtcctccacg aactgaactg
     1681 ccgcc
//
LOCUS       DBNGZC010000448         1330 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1993455, whole genome shotgun sequence.
ACCESSION   DBNGZC010000448 DBNGZC010000000
VERSION     DBNGZC010000448.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1330)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1993455"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="AC16G0_07880"
     CDS             complement(<1..255)
                     /locus_tag="AC16G0_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIR5099573.1"
                     /translation="MSKPIIAVTMGDPAGIGPEIVVRALGEAAVRECCQPVVVGDPRI
                     FARAVELVKSPLRVRTVKSSREAGADPSTLDILTAGEVDPA"
     gene            complement(280..525)
                     /locus_tag="AC16G0_07885"
     CDS             complement(280..525)
                     /locus_tag="AC16G0_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR5099574.1"
                     /translation="MRGRAGSVPIHSRVETYPRVRIRSGACMPGDPSPPESQLCHQFR
                     VERVTVRQALSRPVFEGLILRQRGRAGVVADPRHRHL"
     gene            705..1190
                     /locus_tag="AC16G0_07890"
     CDS             705..1190
                     /locus_tag="AC16G0_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HIR5099575.1"
                     /translation="MRKAGLWWSMGAVVILGMGIQIVPYGHARINPPVHQEPTWNNSV
                     TRDLAVRACYDCHSNQTIWPWYSQVAPVSWFIQRDVDVGRRRLNFSEWDRPKQGTRNL
                     ARRIQRGSMPPWYYLLMHSGAKLSDAEKQALVQGLEETVRQSPTLPAKAGGRDGKGRE
                     G"
BASE COUNT          251 a          406 c          439 g          234 t
ORIGIN      
        1 cgccgggtcc acctcaccgg cggtcaggat gtccagggtg gaggggtcgg ccccggcctc
       61 tcgtgagctt ttgaccgtgc ggacacgcaa cgggctcttc accagctcga cggcgcgcgc
      121 gaatattcgc gggtcgccga ccaccacagg ctgacaacac tctcggaccg cggcctcgcc
      181 caaggcgcga accacgatct ccggcccgat gcccgccggg tctcccatcg tgacggctat
      241 gatcggtttg ctcatgcctg ctctcctctg gtctgttctt caaagatggc gatggcgcgg
      301 atcggcgacg acaccagccc gccctcgttg ccggaggatc aatccttcga acacaggacg
      361 ggaaagggcc tgccggacgg tcacacgctc gacgcggaac tggtggcaga gttgcgattc
      421 cggcggggac gggtcgccgg gcatgcaggc cccggagcgg atgcgaaccc gcggatacgt
      481 ctcgacccgg gagtggatgg gaacggagcc agccctgccg cgcatgctcg cttccgtgag
      541 ggtgggcgca aatctattgt ctaggcctat cccatgtcaa ggaggatttg cacggcacgc
      601 acggcatgga gagaccagta ggaatcaccc tcttgccagg tcagctttaa cctcggtaca
      661 ctccttcgac tggaacacga gatccagaag aaggaggaag tggaatgcgc aaggctggcc
      721 tatggtggtc gatgggagcg gtggtgatcc tcggaatggg cattcaaatc gtgccgtacg
      781 ggcacgcccg catcaatcca cccgtccacc aggagcctac ctggaacaac tctgtgaccc
      841 gcgatctggc cgttcgcgcc tgctacgact gccacagcaa ccagacgatc tggccctggt
      901 attcccaggt tgcccctgtg tcctggttca tccagcgcga cgtggacgtc ggacgccgga
      961 ggctgaactt ttcggagtgg gaccggccaa agcaggggac ccgaaacctg gctcggcgca
     1021 tccagcgcgg ctccatgccc ccatggtact atctcctgat gcactcgggg gcaaagctct
     1081 ccgacgcgga gaagcaggca ctcgtgcaag gcctggagga gaccgtgagg cagagtccca
     1141 cattacctgc gaaggcggga gggcgggacg gcaaggggag agaggggtag gatctccgcc
     1201 gcaaagtgca cctctgatga ttttctggat tgataagagc tggtcaggct ttgcgatctt
     1261 ggcggccttg gcggtggaat ttccgggttc agttgggcga gccgccgctc agggcgcaga
     1321 tgatctggat
//
LOCUS       DBNGZC010000449         2176 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_624098, whole genome shotgun sequence.
ACCESSION   DBNGZC010000449 DBNGZC010000000
VERSION     DBNGZC010000449.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_624098"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="AC16G0_07895"
     CDS             <1..273
                     /locus_tag="AC16G0_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5099576.1"
                     /translation="TPDSKPAARKSKVTKGADQRLKQAQRASILLKHVSDPTRLQVIL
                     ILAEGEQHVGALCEHLSQSQPAVSHHLALLRHGGIIAPRRQGKNNQ"
     gene            complement(286..711)
                     /locus_tag="AC16G0_07900"
     CDS             complement(286..711)
                     /locus_tag="AC16G0_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099577.1"
                     /translation="MLARFEETADPIRHGMATVMLSFLAGLFVGEEEYDGVRDNLDLE
                     RWFRVPKSHERRIHGRRHAGVRIVVGGPTLVHALDAHAAHPEPFTVADLLPYRTAREP
                     PSQTQALNRRKIRRKARSKKRRPALLADLERRYRESATS"
     gene            complement(784..1974)
                     /locus_tag="AC16G0_07905"
     CDS             complement(784..1974)
                     /locus_tag="AC16G0_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099578.1"
                     /translation="MSYDWPEDTDFALWALNVLDRKCPPCGRMRHVCDHRSRRFHTLQ
                     GPVELTCRLDHCPDPDCPGHTKTKSPELEVTLALPKWAIGWDVFCWIGHRRCSRHWAI
                     PQIRGELRDDYGICLSEDSIARYIHHYQVMLAARQQDPEALGRQYRSAGEIILSIDGL
                     QPEKGHETRYVVRELTQKRIWFAEPLLSATADEVRHLIARAKQWAESLGKPVALWLSD
                     KQDAFVTGIAAEFPEVPHRYCDNHFLRDLAKPVLDADSHAKVQMRRKVRGLRAIGQAV
                     LARQKAETRDDGPDDPAATVPATAAASDLPPAVVDPAGGVVLDYCAAVRGILNDDQGG
                     PLHPPGLRMAEALNEVQESIRGNLSANKGGSRRSNSAAWPDASRGAWTKSRSSKRSFE
                     DTSR"
BASE COUNT          382 a          722 c          692 g          380 t
ORIGIN      
        1 acccccgaca gcaaaccggc ggcccgcaag tccaaggtca ccaagggagc cgaccagcgt
       61 ctcaagcagg cgcaacgcgc gtcgatcctg ctcaagcacg tcagcgaccc gactcgcctc
      121 caggtcatcc tcatcctggc cgagggcgag cagcacgtcg gggccttgtg tgagcatttg
      181 agccagagcc agcccgcggt cagccatcac ctggcgctgt tgcgtcacgg cgggatcatc
      241 gcgccgcgac gacagggcaa gaataatcaa tgatccgcaa cagagctaag atgtcgctga
      301 ttcccggtag cgcctctcca ggtcggcaag gagggccggc cgtcgtttct tcgatcgggc
      361 ttttctcctg atcttgcggc ggttcaacgc ctgagtttgg ctcggcggct cgcgggccgt
      421 ccgatacggc aacaggtcgg ccacggtaaa cggctcggga tgcgcggcgt gagcgtccag
      481 ggcatgcacc aacgtcggtc cccccacaac gatccggacc cccgcgtggc gacgaccgtg
      541 gatccgccgc tcgtggctct tcggcacgcg aaaccaacgc tccagatcga gattatccct
      601 gaccccgtca tactcctcct cgccgacgaa caggccggcg aggaagctga gcatcaccgt
      661 ggccatgccg tggcggatcg gatccgcggt ctcctcgaat cgagcgagca atagcctcga
      721 acttctcctc ccgttccgtg atatcctccg tcccgggctc gagggtcgcg gccacctccg
      781 cgatcacctc gacgtatcct cgaatgacct cttgctgctc cttgacttcg tccaggcccc
      841 cctggatgca tccggccagg cggccgagtt gctcctccgc gaaccccctt tgttcgcgct
      901 caagttccct cggatcgact cctggacctc gttcagcgcc tcggccatcc gcaagccggg
      961 aggatgcagg ggtccacctt gatcgtcatt gaggatccca cgcaccgccg cgcaatagtc
     1021 gaggaccacg ccgccggcgg gatcgaccac ggcaggaggc aggtccgacg cggccgcagt
     1081 cgccgggaca gtggctgcgg gatcgtccgg cccgtcgtcc ctcgtctcgg ccttctgtcg
     1141 cgccagtacg gcctgtccga tcgcccgcag gccgcggacc ttgcggcgca tctggacctt
     1201 ggcatgactg tcggcatcca ggaccggctt ggccagatcg cggaggaagt ggttgtcgca
     1261 gtagcgatgg ggcacctcgg ggaactccgc ggcgatcccc gtcacgaatg cgtcctgctt
     1321 gtcggacaac cagagggcca ccggcttgcc caacgattcg gcccactgct tggcccgggc
     1381 gatcaggtgc cggacctcgt ccgcggtggc ggagagcaac ggctcggcga accagatccg
     1441 tttctgggtc agctcccgga cgacgtagcg ggtctcgtgc cccttctcgg gttggaggcc
     1501 gtcgatcgag aggatgatct ccccggccga tcggtattgc cgcccgaggg cctcggggtc
     1561 ttgctgccgc gcggcgagca tcacttgata atggtggatg taccgagcga tcgagtcctc
     1621 cgagagacag atgccatagt cgtcccggag ttcgcctcgg atctggggga tggcccagtg
     1681 gcgcgaacac cggcgatgac cgatccaaca gaagacatcc cagccgatgg cccacttggg
     1741 taaagcgagg gtgacttcga gctcaggact cttggtcttg gtgtgtccgg ggcagtcggg
     1801 atcggggcag tggtcgagcc ggcaagtcaa ctcgactggc ccctggaggg tatgaaaccg
     1861 ccgagaccgg tggtcacaga cgtgcctcat ccgtccgcag ggcgggcact tccgatccag
     1921 gacgttgagc gcccacaagg cgaagtcggt atcctcgggc cagtcgtagg acatcgcgct
     1981 gacctccctc gggcactgag atccgtcgat cgggcagacc agcccagagg ttacacgtgc
     2041 aggagccgag atcctagctc tgtggcgggt cattgaaaaa acaacggtcc ggcaaagggc
     2101 tagaaacccg tgaattcctg acgtaacgtt agaactggtc tcgggaaccg atggctggtt
     2161 catggtcacg gagttc
//
LOCUS       DBNGZC010000450         3115 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1407529, whole genome shotgun sequence.
ACCESSION   DBNGZC010000450 DBNGZC010000000
VERSION     DBNGZC010000450.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1407529"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1018)
                     /locus_tag="AC16G0_07910"
     CDS             complement(11..1018)
                     /locus_tag="AC16G0_07910"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR5099579.1"
                     /translation="MMRALVTGVAGFAGSYLAERLLSQGVEVWGLVQPGGPLRNLEGI
                     RQDSGTEGGLRLIEVDLLEGSDLLRIVGESSPDRVFHLAAAASVRRSLEDPREAFQVN
                     VLGTRNLLEAIRRAGIQPRILMVSSADAYGQSARLPRPLEEDDPLLPVSPYGASKAAA
                     EAVASRYGKEYGLHVIRVRPFPHCGPRQTPQFVLPDWARQLAEIEIGRRPRRLLVGNL
                     SVRRDLSDVRDVVRAYCLALESGESGAVYNVCSGRVHSLRAVLEMLIGLTGLEVEIMA
                     DSERLRPHDLDVLAGSPAAFRLRTGWEAVIPLERTLQDLLDFWRREQPGEAPASEAAA
                     T"
     gene            complement(1015..1899)
                     /gene="rfbD"
                     /locus_tag="AC16G0_07915"
     CDS             complement(1015..1899)
                     /gene="rfbD"
                     /locus_tag="AC16G0_07915"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIR5099580.1"
                     /translation="MRIVVTGARGQLGVELVRALGAGAEVIGCDLPEWDITQADCADR
                     LAALHPAWVVHAAALTDVDRCEREPGLAMTVNAEGTRRVAEGCRRIGAGLVYLSTDYV
                     FDGRKGTPYVEEDLPAPLNAYGRSKLEGETAVRGLAPRWAIVRTAWLYGTHGKNFVKT
                     ICEKAAGGGPLRVVDDQVGSPTYAADLADAMTRLLCRGLTGTYHVTNGGSCSWYEFAR
                     EILRLAGLASTSVIRITSEVLDRLARRPAHSVLANAAWQAAGLPLLRPWPEALAAMLA
                     AVPPRMWARTESRKGRGA"
     gene            complement(2040..2570)
                     /locus_tag="AC16G0_07920"
     CDS             complement(2040..2570)
                     /locus_tag="AC16G0_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HIR5099581.1"
                     /translation="MAAGTTSLGLPAAGSARVIHGVQVKRLTVLPDDRGFLMEMLRED
                     EPIFDRFGQVYITGCRRGVAKGWHYHKEQTDHFVCVGGTALVALYDPRDGSPTRGMIQ
                     EATLHSPPTREPEPLLLKIPPLVVHGFTAIDCEEARIINIPTLPYRRDHPDEYRYLWN
                     SPEIPYRWPTEVVRGG"
     gene            complement(2604..>3115)
                     /locus_tag="AC16G0_07925"
     CDS             complement(2604..>3115)
                     /locus_tag="AC16G0_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase"
                     /protein_id="HIR5099582.1"
                     /translation="GELELSDAHPDLLDHGSQVGVSEITGWWNDTGKPEDLLEANRFV
                     LDQVLDDTGPTIRGEVDAASDIAGKVVVDAGARVIASHIRGPVIIGRDAVIDRSYIGP
                     FTSLGARCVIRDSELEYSILFEDTQILDADVRIERSLLGREVIIRRGDSRPKTQKFIL
                     GDQSIIELC"
BASE COUNT          487 a         1101 c         1022 g          505 t
ORIGIN      
        1 gacccacgag tcaagttgcg gcggcttcgc ttgcgggggc ttcgccgggc tgctcacgac
       61 gccaaaaatc gagaaggtcc tggagggtcc gctccagggg aatgaccgcc tcccagccgg
      121 tgcgcaggcg gaacgccgcg ggtgaccccg ccaggacatc caggtcgtgg gggcgcagcc
      181 gctcgctgtc tgccatgatc tccacctcca gacccgtgag tccgatcagc atctccagca
      241 ccgctcggag gctgtggacc ctcccggagc agacattgta caccgcgccc gactctcccg
      301 actccagcgc caggcagtag gcccgcacga cgtcccggac gtccgagaga tcgcgccgga
      361 cggacagatt cccgacgagg agcctcctgg ggcgccgtcc gatctcgatc tccgccagtt
      421 gccgggccca gtctggcagc acgaactgcg gggtctggcg cgggccgcag tgggggaaag
      481 ggcggacccg gatcacgtgg agcccgtatt cctttccgta gcgggacgcc accgcctccg
      541 ccgccgcctt gctcgccccg taggggctca cggggagcag cgggtcgtcc tcctccaggg
      601 gtcgcggcag gcgcgccgat tgcccgtagg cgtcggcaga gctgaccatc aggatccggg
      661 gctggatccc ggcacgtcgg atggcctcca gaagattccg tgtgccgagg acgttcacct
      721 gaaacgcttc ccgtggatcc tccagggacc gccggaccga ggcggccgcc gccagatgga
      781 atacccggtc ggggctgctc tccccgacga tccgcaggag gtccgaaccc tccaggagat
      841 ccacctcgat gagccggagg ccgccttcgg tcccggagtc ctggcgaatc ccctccaggt
      901 ttcgcagggg gcccccgggc tggaccaatc cccacacttc cacgccctgc gacaggaggc
      961 gttcggccag gtaggatcct gcaaatcccg ccactccggt caccaaggct cgcatcatgc
     1021 gccccgccct ttccggctct ctgtccgagc ccacatccgc ggcgggacgg cggccagcat
     1081 ggcggccagg gcctccggcc agggccgcag cagcggaagg ccggccgcct gccacgcggc
     1141 attcgccagg accgaatgcg cgggtcggcg cgccagccgg tccaacacct ccgaggtgat
     1201 ccggatcacg gatgtcgagg ccaaccccgc cagacgcagg atctctcgcg cgaattcgta
     1261 ccaggagcag gaaccgccgt tggtgacgtg gtaggtcccg gtcagtcctc gacacagcag
     1321 gcgggtcatg gcatcggcaa gatcggcggc atacgtgggc gagcccacct gatcgtccac
     1381 gacgcgcagg ggtcccccgc cggctgcctt ctcgcagatc gtcttcacga agttcttgcc
     1441 gtgcgtcccg tagagccagg cggtccggac gatggcccaa cgcggcgcca ggccccgcac
     1501 cgcggtctct ccctccagct tcgagcggcc gtatgcgttg agcggcgccg gcaggtcctc
     1561 ttccacgtat ggcgttccct tgcgcccgtc gaacacgtag tccgtgctca ggtagaccag
     1621 accggccccg attcggcggc agccctcggc cacccggcgg gtcccttccg cgttcaccgt
     1681 catggcgagt ccaggctcgc gctcgcaccg atccacgtcg gtcagggccg cggcatgcac
     1741 cacccatgcc gggtggagcg ccgccaatcg gtcggcgcag tccgcctgcg tgatgtccca
     1801 ttccggcagg tcgcagccga tcacctcagc gcctgcgccc agggcacgca ccagctcgac
     1861 cccgagttgt ccgcgggcac ccgtcaccac gatccgcatc tgcgagtcca ttgagtctgt
     1921 tgagtccgtt gagtctattg agtgtgtgga atgtgtggag tccgttgcgt ccattgaatc
     1981 cgctgaggcg cgtcgagttc tcggaggctc tccgacgggg gagactcgat agaccgtaat
     2041 tagcctccgc ggacgacctc ggtgggccag cggtacggga tctccgggct gttccagaga
     2101 tagcgatact cgtcggggtg gtcgcgacgg tatgggaggg tcgggatgtt gatgatgcgc
     2161 gcctcctcgc agtcgatggc ggtgaatcca tggacgacga gcggggggat cttcagcaag
     2221 agcggctccg gttccctggt cggcggggaa tgcagggtcg cctcctggat cattccccgg
     2281 gtgggcgagc cgtcacgcgg gtcgtagagg gccaccaggg cggtgccgcc cacacacacg
     2341 aaatggtcgg tctgctcctt gtgataatgc cagcccttgg ccacgccgcg ccggcagccc
     2401 gtgatgtaca cctgaccgaa gcgatcgaag atcggctcat cctcgcgcag catctccatg
     2461 aggaaccccc ggtcgtcggg caggaccgtg agccgcttca cctggacccc gtggatcacc
     2521 ctcgcactcc ccgcggccgg caaaccgaga gacgttgtcc cggcagccat ctcgccttcc
     2581 cgtgcacctg cgacacgcag aggctaacac agctcgatga tgctctgatc ccctaggatg
     2641 aacttctgcg tcttgggccg cgagtccccg cggcggatga tcacttctcg gcccaggagg
     2701 ctgcgctcga tccgcacgtc cgcatccagg atctgggtgt cctcgaagag gatgctgtac
     2761 tccagctcgc tgtcgcggat gacgcagcgc gcccccaggg aggtgaaggg cccgatgtag
     2821 ctccggtcga tgacggcgtc ccgcccgatg atgaccggtc cgcggatgtg gctggcgatc
     2881 acccgcgccc ccgcgtccac gaccaccttg ccggcgatgt cgctggcggc gtccacctcg
     2941 ccacggatag tgggccccgt gtcatccagc acctgatcca ggacgaagcg gttggcttcg
     3001 aggagatcct ccggctttcc cgtgtcgttc caccagcccg tgatctccga gacgccgact
     3061 tgcgagccgt ggtccaggag atccgggtgg gcgtcggaga gctccagctc gcctc
//
LOCUS       DBNGZC010000451         2587 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_919095, whole genome shotgun sequence.
ACCESSION   DBNGZC010000451 DBNGZC010000000
VERSION     DBNGZC010000451.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_919095"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1238)
                     /locus_tag="AC16G0_07930"
     CDS             complement(69..1238)
                     /locus_tag="AC16G0_07930"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099583.1"
                     /translation="MAIPLTYNLRSLKVRWTSTVVAVLGIAGTVGVFVAMLSLAQGFK
                     ATLVASGSTHNAIVRRAGATSEMESVVSLDQVRVIQDAPGIGRGRLGPLVSPEVVVIA
                     AFPLKSSGTDANVQVRGVSPQVLSVRDSVRIISGRFFQPGLAELVVGRNVAQSYVGFA
                     LGKTVRFGGGTWTVVGVFDAGGSAFDSELWCDANVLNQVYQRPQNIFQSVTARLASPE
                     ALTTFKDALTSDPRITVQVDREVDYYEKQSRQLTTLITVRGTIVALVMGIGAVFGALN
                     TMYSAVVERSREIATMRAVGFGVGSVVMSFLFEALCIAFVGGVLGCAAVLPLNNLTTG
                     TMNWQTFSHLAFAFRVTPPLLASGLLFALLMGLVGGVPPAIRAARQPIALALREL"
     gene            complement(1240..2346)
                     /locus_tag="AC16G0_07935"
     CDS             complement(1240..2346)
                     /locus_tag="AC16G0_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099584.1"
                     /translation="MRKKIRTVLTAGSFAVALFLFGILAVVRGAFSQGVDVAGADRLV
                     VVNKVSIIQPLPLSYRDRLLRIPGVTQATFASWFGGVYQDERNFFPEFAIDREKYRQM
                     FSEFIIPDDQWQAFLADREGCIVGQGLAERFKWKLGDRIPIKGTIFPGAWEFNIRGIY
                     HGKRLQDDTTQFWFRWDYLDERTPYRKGYVGWYTVRIANPDDAARIVRTIDEDFANSP
                     WETKTDTEKAFAASFVKQMGNIQFLIMSIGGVVFFTLLLVTGNTMAIAVRERGRELAV
                     LKAIGFSDSFTLVLVIAETLVVSAVGGGIGLALAKLFTLRGDPTHGMLPFFYLPSEAM
                     ALGFVLAMVVGLMAGILPAFSAMRLRVVDALRRV"
     gene            complement(2376..>2587)
                     /locus_tag="AC16G0_07940"
     CDS             complement(2376..>2587)
                     /locus_tag="AC16G0_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099585.1"
                     /translation="TFLLCDEPTGDLDRKSADEILDLLGRLVREFGKTVLMVTHDPLA
                     AERAHVVLHLEKGMLVDAGTAGRVT"
BASE COUNT          510 a          878 c          786 g          413 t
ORIGIN      
        1 ctttctgtga cgcaaagtac cggcgtgcgc caggatcgcg gctccagtgg gaatcgggaa
       61 gtgatggctc agagctcccg gagtgccagg gcgatgggct ggcgggctgc gcggattgcg
      121 gggggcacgc caccgacgag ccccatcagc agggcaaaca gcaatcccga ggccagcagg
      181 ggtggggtca ctcggaaggc gaaggccagg tgggagaagg tctgccagtt catggttccc
      241 gtcgtcaggt tgttcaaggg caggacggcc gcacagccca gcactcctcc gacaaaggca
      301 atgcagagcg cctcaaagag gaaggacatc accacgctcc cgacgccgaa tcctaccgcg
      361 cgcatggtgg cgatttcccg cgagcgctcc accaccgccg agtacatcgt gttcaaggcg
      421 ccgaacaccg ccccgatgcc catgaccaac gccacgatcg taccccgcac cgtgatcaac
      481 gtcgtcagtt ggcgcgactg cttctcgtaa taatccacct cccgatccac ctggaccgtg
      541 atccgcggat cggaggtgag cgcatccttg aacgtggtga gcgcctccgg ggacgcgagt
      601 cttgccgtga ctgactggaa gatgttctgc ggacgctggt agacctgatt gagcacattg
      661 gcgtcgcacc acagctcgga gtcgaaggcg ctgccgccgg cgtcgaagac acccaccacg
      721 gtccaggtcc cgcccccgaa ccggacggtc ttgcccaatg cgaacccgac atagctttgg
      781 gccacgttcc gccccacaac cagctcggcc agtccgggct ggaagaaccg cccgctgata
      841 atcctcaccg aatctcgcac cgacagcacc tgaggcgaga cccctcggac ctgcacattc
      901 gcatcggtcc cgctgctctt gagtggaaat gccgctatca cgacaacttc ggggctgacc
      961 agtggaccca ggcggccccg cccgataccc ggggcatcct ggatgacacg cacctggtcg
     1021 agcgagacca cgctctccat ctcggaggtg gcgccggccc gtcgcacaat cgcgttgtgg
     1081 gtggagcccg atgccaccag cgtggccttg aatccctgcg ccagcgagag catggccaca
     1141 aagaccccca cggtgccggc gatgcccagc acggccacca ccgtcgaggt ccagcggacc
     1201 ttcaggctgc gcaggttgta cgtcagggga atggccacgt cagaccctcc gcaaggcgtc
     1261 aaccacccgg agccgcatgg cggagaatgc aggcaggatg ccggccatca gtcccaccac
     1321 catcgccagc acgaacccga gggccatggc ttccgacggc aaatagaaga acggcagcat
     1381 gccgtgggtt ggatcccccc gcagcgtgaa cagcttcgcc agggcgagcc cgattcctcc
     1441 cccgacagca gacacgacca gcgtctccgc gatgaccagg accagagtga aactgtcgga
     1501 gaaaccgatc gccttcagca ccgccagttc ccgcccgcgc tcgcgcacgg cgatggccat
     1561 cgtgttcccg gtcaccagca agagcgtgaa gaacaccaca cccccaatgc tcatgatcag
     1621 gaactggatg ttgcccatct gcttcacaaa ggacgcggcg aaggctttct ccgtgtcggt
     1681 cttggtctcc cacggagagt tggcgaagtc ttcgtcgatc gtcctcacga tacgcgcggc
     1741 atcgtcgggg ttggcgatgc ggaccgtgta ccagcccacg taccccttcc ggtacggggt
     1801 ccgctcatcc agatagtccc agcgaaacca gaattgcgtg gtgtcgtcct ggagccgctt
     1861 tccgtggtag atgccccgga tgttgaactc ccacgccccc gggaagatgg tgcccttgat
     1921 ggggatccgg tcgcccagct tccacttgaa ccgctccgcc aggccctgcc ccacgatgca
     1981 gccttcccga tcggcgagga atgcctgcca ctggtcgtcg ggtatgatga actcagaaaa
     2041 catctgccgg tacttctctc gatctatggc gaactcgggg aagaagttgc gctcgtcttg
     2101 atagacgccc ccgaaccagc tggcgaatgt tgcctgcgtc acccctggga ttcgcagcag
     2161 ccggtcacgg taagacagcg gcagaggctg gatgatggag accttgttca ccacgaccag
     2221 ccggtcagcg ccggcgacgt ccaccccctg gctgaatgcc ccccgcacca cggccaggat
     2281 gccgaacagg aacagggcca cggcgaagga acccgccgtc aggacggtcc ggattttctt
     2341 ccgcaacaaa ttggctagga tgagacgagc gtacttcacg tgacgcgtcc tgccgtcccg
     2401 gcgtccacca gcatgccctt ctccagatgc agcacaacat gggcgcgttc ggcggccagg
     2461 gggtcgtggg tcaccatgag cacggtcttg ccgaactccc gaaccagccg gccgagcaag
     2521 tcgaggatct cgtccgcgct cttgcggtcc aggtcgccgg tcggctcgtc gcagagcagg
     2581 aaggtcg
//
LOCUS       DBNGZC010000452         2299 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_721746, whole genome shotgun sequence.
ACCESSION   DBNGZC010000452 DBNGZC010000000
VERSION     DBNGZC010000452.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_721746"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1329)
                     /locus_tag="AC16G0_07945"
     CDS             complement(<1..1329)
                     /locus_tag="AC16G0_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR5099586.1"
                     /translation="MESNLYRYIFRRSLRMQVALIVAIFALGFLNPYVLVLTKRTINA
                     IRQMHISDTLHLLMWYLAAVLATGGLKYLKQNLEGIVSETMLRDLRTELYHRILRFPL
                     PYVRSTSAGQLVAMILGETEDLGQFFGEAFSVPMFNGLMLLGSVGFMLWQNPWLAMAA
                     MVLFPIQIWLVRKIQRRVIELSRKRVRLVRGLSDRIQESVGGLQEIHADDTLAYESSG
                     FRGKLHKIFKVRIRIYNLKYLVKWFNNFLEKLGIFFLLLIGGWLIIKRPGSFDLGALV
                     AFIEAFKQLNEPWRELINYIQQRDTARVKYEQVIVSFDPPGLRPEFPLDERLPEPVPQ
                     LAGAYDVRGASVILDGTTRVLDRLQLSTPPYRHVAIVGPTGSGKSTLTMVLARLSGYT
                     GTALLDNMELAQVPSAVAGRQIAYVGSDARLFTGTIFENLVYSLRHRPQAP"
     gene            complement(1357..>2299)
                     /locus_tag="AC16G0_07950"
     CDS             complement(1357..>2299)
                     /locus_tag="AC16G0_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019815238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099587.1"
                     /translation="ALRTRHADFIYFPPQRVYFFQDGRFTRPFVYGYRMALNMDNLKR
                     EYREDRSRRYRLLLLPRGESYWMGGLWHSDLHLFGVEEGGTLFLLGTDRLGRDMLSRI
                     IHGTRISLTVGLIGITVSFVLALIMGGLSGYYGGMLDIAIQRLIEVIRSFPELPLWMA
                     LSAALPARLDPVLVYVGITVILGVIEWTGLARSVRGKLLAVREEDFVTGAVLAGGTPA
                     YVIRKHLLPAFTSHLIVSATLSIPRMILGETALSFLGLGLRPPVTSWGVLLSEAQNMN
                     AVALYPWLLFPVVMVVLTVLAFNFFGDGLRDAADPYT"
BASE COUNT          497 a          695 c          733 g          374 t
ORIGIN      
        1 gggggcctgg gggcgatgcc ggagactgta gaccagattc tcaaagatcg tgcccgtgaa
       61 cagccgcgca tcgctgccga cataggcgat ctgacgacct gccacggctg atgggacctg
      121 ggcgagctcc atgttgtcca gcagggcggt gcccgtatat ccggaaagtc tggccaatac
      181 catggtcaag gtgctcttgc cgctcccggt agggccgacg atggccacgt gccggtaggg
      241 tggggtcgag agttggaggc ggtccaagac ccgcgtcgta ccgtccagga tcacgctggc
      301 tccccggaca tcataggccc cggccaactg ggggaccggc tccggaagcc gctcgtcgag
      361 agggaactcg ggacggaggc ccggtggatc gaaactgacg atcacctgct cgtacttgac
      421 cctggcagtg tcgcgctgct ggatgtagtt gatgagctct cgccagggct cgttcagttg
      481 cttgaaggcc tcgatgaagg ccaccaaggc cccaaggtcg aaagagccgg ggcgctttat
      541 gataagccag ccgccgatca ggagcaggaa gaagatgccc agcttctcca ggaagttgtt
      601 gaaccacttg accaggtact tgagattgta gatccggatc cggaccttga atatcttgtg
      661 aagcttgccg cggaagccgc tggattcgta agccaaggtg tcgtcggcgt ggatctcctg
      721 aaggccgccc accgattcct ggatgcggtc agagagtccg cggaccagcc ggaccctctt
      781 ccgggacagc tcaatcactc gccgctggat tttccggacc aaccagatct gaatcggaaa
      841 gagaaccatg gccgccatgg ctagccacgg gttctgccac aacatgaacc cgacagagcc
      901 gagaagcatc aagccgttga acatgggcac cgaaaaggcc tcaccgaaga attgccccag
      961 gtcttcggtt tcccccagga tcatggccac gagttgcccc gccgaggtgc tgcggacgta
     1021 ggggaggggg aagcgcagga tccgatggta caattcggtc cggagatccc gcagcatggt
     1081 ctccgatacg attccctcca ggttctgctt gagatatttc aatcccccgg tggccaggac
     1141 ggcggccagg taccacatga gcagatgcaa ggtatcgctg atgtgcatct ggcggatggc
     1201 gttgatggtc cgcttggtca ggaccaggac ataggggttc aggaacccca aggcgaagat
     1261 ggcaacgatc aacgccacct gcatccgaag gctgcgccga aaaatgtagc ggtagaggtt
     1321 ggactccatg gtcctacctt tcaggcggcc gaaggcttaa gtgtacgggt ccgccgcatc
     1381 gcgaaggccg tccccgaaga aattgaaagc caggacggtc agcaccacca tgacgacggg
     1441 gaacagcagc caggggtaga gggcgaccgc gttcatgttc tgggcctcgg agagcagcac
     1501 cccccaactg gtgaccgggg gccgcaaccc caggcccagg aagctgaggg ccgtctctcc
     1561 caggatcatg cgcgggatgc tcaacgtggc ggacacgatg aggtgacttg tgaaggcggg
     1621 cagcaggtgc ttgcggatca cgtaggcggg tgtacccccg gccaggaccg ccccggtgac
     1681 gaaatcctcc tcccgcacgg ccaagagctt cccccgcacg ctcctggcga gcccggtcca
     1741 ctcgatgacc cccaggatga cggtaatgcc cacgtaaacc aggaccggat ccaaccgggc
     1801 cggcagcgcc gcggagagcg ccatccacag cggcaactcg ggaaaggacc ggatcacctc
     1861 gatgagccgt tggatggcga tgtccagcat gccgccgtag tagcctgaaa gccctcccat
     1921 gatcagggcc agcacaaaac tcacggtgat cccgatgagg ccgacggtca gcgagatccg
     1981 cgtcccatga atgatgcggg agagcatgtc ccggccgagc cggtcggtcc ccagcaaaaa
     2041 cagcgtgccg ccttcctcga cgccgaagag gtgcagatcc gaatgccaca gaccccccat
     2101 ccaatacgac tccccccgcg gcaggagaag cagccgatag cgtcgcgagc ggtcttcgcg
     2161 gtactcgcgc ttcagattat ccatgttgag ggccatgcgg tagccgtaga caaagggcct
     2221 ggtgaagcgc ccatcctgga agaagtaaac ccgttgaggc gggaaataga taaagtccgc
     2281 atgccgcgtg cgcagcgca
//
LOCUS       DBNGZC010000453         3905 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2679348, whole genome shotgun sequence.
ACCESSION   DBNGZC010000453 DBNGZC010000000
VERSION     DBNGZC010000453.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2679348"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..607
                     /locus_tag="AC16G0_07955"
     CDS             164..607
                     /locus_tag="AC16G0_07955"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5099588.1"
                     /translation="MIDLARALWAEVEEAAVRLRAFGETDVTRDRGPGKWTRKEILGH
                     LIDSAANNHQRFVRAQFVSPVVWPGSDPGSWVLLHRYRARPWMELVDLWVAFNRQVAQ
                     VIEYVPAEKLQTPCTIGDNTPASLEWWMRDYLRHLKHHLAQIEGD"
     gene            complement(1150..1374)
                     /locus_tag="AC16G0_07960"
     CDS             complement(1150..1374)
                     /locus_tag="AC16G0_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099589.1"
                     /translation="MDLDLPGAACTLEDVLAALAGAEPRIARYLRGEDGQPPASLRPL
                     LDDLLLEPGTPIPDGATVTLLYATAGGTSK"
     gene            complement(1720..3030)
                     /locus_tag="AC16G0_07965"
     CDS             complement(1720..3030)
                     /locus_tag="AC16G0_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007258605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HIR5099590.1"
                     /translation="MESKGVRNRIVVEEYTGGLIGPSLEMLGTLKDGGTISTVSPPGC
                     WGPMITPEFVGGHEVSRPVAVEGAHVGDAVAIFIQKVWVRSQATCSGTMTTKAAAFGS
                     DPFVEKHCPNCKTPWPASRIEGTGPDCVRCVKCGAECSPFDFEEGYTIAFDDRRRMGI
                     TVDEKAASDFATRAYEVSALPKQARQHPILLYKPHTIPGTLSRLQSFIGNIGTIPSKD
                     MPDSHNAGDFGSFLVGAKHRFGLTQSELDQHRTDCHLDCNAVRDGAVLICPVKVEGAG
                     IYIGDSHANQGDGELSLHTTDITADVTVKVKVLKGLKLEGPILLPVPEDLPWIARPYS
                     KKELEIGERLAKQHSVTLQRKKGPIQVIGTGPTINEATDNAIGRAARLLGMSQPEVRN
                     RCTITGAVEIARLPGAVQLTILAPLPRLDEVGLGKLVRRQYGIA"
     gene            3381..>3905
                     /locus_tag="AC16G0_07970"
     CDS             3381..>3905
                     /locus_tag="AC16G0_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HIR5099591.1"
                     /translation="MREYERIRAAIIRGGTSKGVYLLANELPQDPGARDKVILSIFGS
                     PDPRQINGLGGADPLTRKVAIIAPSRREGADVDYTFGYVGIEKAVVDYDGNCGNISQG
                     VGPFAVDEGLVPVTEPITKVRIFNTNTKKVIEAAVPVKDGKALTQGDFFVNGVPGTGA
                     KIVLNFVNSGGSRTG"
BASE COUNT          724 a         1192 c         1282 g          707 t
ORIGIN      
        1 ctgaaagaat ggctctgcga ctggacgccc gaagccttcg atctccaagc cgtgaagcgc
       61 accttcgacg gctgatcccc cggtgggagg cgctgtatga ggcagacgca agggggaggc
      121 gggtcaacgc ggggctcgaa acgcgttcgg gtggaggaat cgcatgatcg atctggcgcg
      181 agcactatgg gccgaagtgg aagaggcggc agtgcggctc agagcctttg gggaaaccga
      241 tgtcacccgt gaccggggcc ccggcaagtg gacgaggaag gagattctgg gtcatctgat
      301 cgactccgcc gccaacaatc accagcggtt cgtgcgagcg cagttcgtga gccccgtcgt
      361 ctggcccggg tccgacccgg ggtcgtgggt attgttacac cggtatcgcg cccgcccctg
      421 gatggagttg gttgacctct gggtggcttt taatcgtcag gtggctcagg tcatagaata
      481 tgtgccggcc gagaagctac agacgccctg caccatcggc gacaatacgc ccgcctcgct
      541 cgagtggtgg atgcgggact atctgaggca cctgaagcac catttggccc agatagaggg
      601 tgattgaaac ggtgccgccc gagcaggcgc tctagcgaat cgggcccatg cgggcacgtc
      661 cgatcttccg gcctggccgg tattcgaggc gaactgtagg ttgtgatcat tggcggtacc
      721 tggtaccgat gaacggctaa gcagcggaat tcgctcaggc cgagttcgaa ttcatcaaga
      781 cggaggtgat tcggagggga agcggtagag tacccgctcg gggtgaacca gcgaacgtgc
      841 ggttgacaat ccggccttca tcgagcggtc cccgccccgc cgctctcgag aatgaattgg
      901 aacccgtgcc gaggcgaatt aatgacttag ccgccatttg gtaccaggta ccgcacaagg
      961 gtcacgattg tgagatgtgt tcgcataacc cgcctccttt cgcatgcggc gctgcttcac
     1021 acaagaaaaa tccacgccat tcggcaaaag gtgcgtcggt ctttccgtac ggcctaaagc
     1081 acatttcgtc ttctggcggg ctacctttct gccgtgtaac ggcggtccgt atgtgccgga
     1141 gccaatggat tatttgctcg ttccaccggc cgtggcgtag aggagggtaa cggtggcgcc
     1201 gtcggggatg ggggtgccgg gttcgaggag gaggtcgtcg aggagagggc ggagcgaggc
     1261 gggcggctgc ccgtcctctc cccggagata gcgggcgata cggggctcgg cgcctgccag
     1321 ggcggcgagg acatcttcca gggtgcacgc cgcacccggc aggtccaggt ccacggccga
     1381 tcgggcgagg atcacacgcg gtgggcccgt caaggtgacg cgtatccgca caaggcacca
     1441 gtcagaagat aatccgcacg aatccttctt ttaccacaga cccagggacc atggaaatcg
     1501 gggggagggc agaggatcaa ccccagccct gcccccgtct cagattagca gaatcttgaa
     1561 taaccgttct tcccacgggc tgctcaaaaa ggtccagatg caaggcggcg cacccatctt
     1621 tcgatgggta cccggcgagg cgcgaggcgt actgactccg tacgccgcag tgccgcgcga
     1681 gcgcgccaac gcagcagatg gggctttttc agcagtccgt taagcaatcc cgtactgccg
     1741 ccggaccagc ttgcccaggc cgacctcgtc cagacgcggg agtggcgcca ggatggtgag
     1801 ctggacagcg ccgggcaggc gggcgatctc caccgcgccg gtaatggtgc agcggtttcg
     1861 cacctcgggc tgggacatcc cgagcaggcg cgcggcccgg ccgatggcgt tgtcggtggc
     1921 ctcgttgatc gtcgggccgg tcccgatcac ctgaatcggt cccttcttgc gctgaagcgt
     1981 cacgctgtgt tgcttggcca gccgttcgcc gatctccagc tccttcttgg agtagggccg
     2041 cgcgatccag ggtaggtcct cgggtacggg gagcaggatc ggtccctcca gctttaagcc
     2101 cttgagcacc ttcaccttca ccgtcacgtc ggcggtaatg tcggtggtgt gaagcgacag
     2161 ctccccgtcg ccctggttgg cgtgcgaatc accgatgtag atcccggccc cctccacctt
     2221 cacggggcag ataagcaccg ccccgtcccg cacggcgttg caatccaggt ggcagtcggt
     2281 gcggtgctga tcaagctcgg attgggtcag gccgaaacgg tgcttggccc cgaccaggaa
     2341 tgagccgaag tccccagcgt tgtgggaatc cggcatgtcc ttggacggga tcgtgccgat
     2401 gttgccgatg aaggactgga gccgcgagag cgtcccgggg attgtgtgcg gcttgtacag
     2461 gaggatcggg tgctgccggg cctgtttggg aagagcggac acctcgtagg cgcgggtggc
     2521 aaaatccgag gcggccttct cgtccaccgt gatgcccatt cgccgccggt cgtcgaaggc
     2581 gatggtgtag ccctcctcga aatcgaaggg actgcactcg gcgccacact tgacgcagcg
     2641 gacgcagtcc ggaccggttc cctcgatccg gctggccggc caaggcgtct tgcagttcgg
     2701 gcagtgcttc tccacgaatg gatccgatcc aaaggccgcc gccttggtgg tcatggtgcc
     2761 agagcaggtc gcctgcgacc ggacccagac cttctggatg aaaatagcca cagcatcccc
     2821 gacgtgagcc ccctccacgg ccaccggccg tgagacttcg tgcccaccga cgaactccgg
     2881 cgtgatcatg gggccccaac atcccggcgg tgatactgtg ctgatggtac ctccgtcctt
     2941 cagcgttccg agcatctcca ggctggggcc gatcaggccc ccggtgtact cctcgacgac
     3001 gatccgattt cgtaccccct tgctttcgat cccggccatg gctccctccc ccgggccgca
     3061 tattagaccg ccgtggcccg atagactgtg tgtaccaggc tatgatccga gcgtcaaggg
     3121 cggcccgctg gcggtggccc gctggcggta ccaggactgt cagtatatcg ccatcacggg
     3181 gcaaaacctg cctcctgctt gtccctttgt tcttgcctcc ctacagcgcc tccgaggttt
     3241 attgacattc ccatccagct catgatagtt ccgccagtag ctgctcgaag attctgcagg
     3301 cggatgaaac aaacctggaa tgggggacgc ggtccttctg aattgaaggc cagcgaacga
     3361 acaagggaga cacgtggatg atgcgtgagt acgaaagaat ccgggcagcc atcatccgag
     3421 gggggacgag caagggagtc tacttgctgg ccaacgagct gccgcaggat cccggagcgc
     3481 gagacaaggt catcctcagc atcttcggca gcccggatcc acggcagatc aacggcctgg
     3541 ggggcgcgga tcccttgacg cgcaaggttg cgatcatcgc gccctctcgg cgcgagggcg
     3601 ccgacgtgga ctacaccttc ggctacgtgg ggatcgagaa ggccgtcgtg gactacgacg
     3661 gcaactgcgg gaacatctcc cagggcgtcg gcccctttgc cgtagacgaa gggctggtcc
     3721 ccgtcaccga accgatcacg aaggtgcgca tcttcaacac caacaccaag aaggtgatcg
     3781 aggcggccgt gccggtcaag gacggcaagg ccctcaccca gggggacttc ttcgtcaacg
     3841 gggtgccggg cacgggcgcc aagatcgtcc tgaacttcgt gaattccggc gggtccagga
     3901 cggga
//
LOCUS       DBNGZC010000454         4382 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_334451, whole genome shotgun sequence.
ACCESSION   DBNGZC010000454 DBNGZC010000000
VERSION     DBNGZC010000454.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4382)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4382)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4382
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_334451"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="AC16G0_07975"
     CDS             <1..751
                     /locus_tag="AC16G0_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099592.1"
                     /translation="WFDDFSAILNNARLAAQVARGGKAQGILIDVEQYKAPLFEYRKQ
                     RDAATKSWSEYAAQVRQRGREVMNAFQQGYPDVTVFLTFGYSVPWRESQRGKKPLPDG
                     EYGLLAPFLDGMVEAARGRTRLVDGYEIAYYHNKDTSKFAAAYQTIRDELLPIVANPK
                     EYRRRFSVSFGLWMDFDSTAIGWDGEDGSRNFYTPEEFGRSVRKALEVADEYVWIYSQ
                     IPRWWSSEGKTIKLPEGYADALRQARQGLAP"
     gene            complement(899..1903)
                     /locus_tag="AC16G0_07980"
     CDS             complement(899..1903)
                     /locus_tag="AC16G0_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019988444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR5099593.1"
                     /translation="MHAIRLHQPGGPEQLRFEETPTPRPGPGEVLVHLKAAALNHRDV
                     WIRLGRQMTDRLPLIPGSDGAGLVAEVGPAVSHLRSGQEVVINPSLDWGDREDRPSSA
                     FRILGGPDPGTYAEYIVVPAGNVFPKPSPLSFEEAAAMPLASLTAWRAVVTRGQVRPG
                     ERVVVLGIGGGVATFALQIARQAGASVIATSSSDDKLLRARELGADLTINYTSTAWDE
                     VVLKHTGGGADVIIDSVGKATWEQDLRALRPGGRLVTFGATTGPTTEVSIRTVFWNQL
                     SILGTTMGSPREFAAMLSLYETGRLKPIVDRVFPLREAAEAHRRMDAGEQFGKLVLVP
                     "
     gene            2227..3024
                     /locus_tag="AC16G0_07985"
     CDS             2227..3024
                     /locus_tag="AC16G0_07985"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014743950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5099594.1"
                     /translation="MGSNLFDLGGRVAVVTGGGTGLGKGIATGLAQAGALVILAGRRQ
                     DVVEQATAEIRAGGGRAEGVTLDVTRIQELPAFFEAVSTRHGGLDILVNNAGTNRRNP
                     CLEYTEQDWDTVMNLNLKAVFFCCQAAARIMKERGGGKIINTASLASTVTSTNQAAYA
                     PSKGGVRLLTMHLALELAPYRINVNAIGPGWFRTPLNDDLFRNEGWARGVRALIPWGR
                     TGTPDDLAGTAVFLASRASDYVTGQVIYVDGGMLAGYKVHPISTQEV"
     gene            3138..3830
                     /locus_tag="AC16G0_07990"
     CDS             3138..3830
                     /locus_tag="AC16G0_07990"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR5099595.1"
                     /translation="MRIFDVTLPIHPGMPVYEGDPEVSIRKWFSLDGGEAANVSVLSL
                     GSHTGTHVDAPLHFRNGAPGVDRLPLDVLIGPARVYALALADHLDATSLRGLNLGAYP
                     RVLFKTRNSAASREGRSSKDFVALTADGARLLVEVGVQLVGLDGPSADCFSSTDFPVH
                     HTLLDAGVIILEGLDLSPVPPGDYELLCLPLMIRGGDGAPARVVLRERTEGPAPPLAG
                     GIQRRTHISPGR"
     gene            3845..>4382
                     /locus_tag="AC16G0_07995"
     CDS             3845..>4382
                     /locus_tag="AC16G0_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR5099596.1"
                     /translation="MRRTKIVCTIGPATSSPEMIGQLVEAGMNVARLNFSHGAWEEHA
                     GVIASLRQISARKHVPVAILQDLQGPKIRLGTFRGGRASLQPGAEFVITVRPVEGTAA
                     QASTTYDQLPRDVKPGDRILLADGTIELKALESTEDWVRCRVVVGGEISDHKGINLPG
                     VSVSARSMTAKDREDLRFG"
BASE COUNT          773 a         1383 c         1492 g          734 t
ORIGIN      
        1 ctggttcgac gatttctcgg cgatcctcaa taatgcccgc ctggccgcgc aggtcgcccg
       61 cggcgggaag gctcagggca tcctgattga tgtcgagcaa tacaaggcgc cactatttga
      121 ataccgcaag cagcgcgatg ccgccacgaa gtcgtggagc gagtacgccg ctcaagtgcg
      181 ccagcgtggc cgggaggtga tgaacgcctt ccagcagggc tatccggatg tgacggtctt
      241 cctcacgttc ggctacagcg tgccctggcg agaatcacag cgaggcaaga agccgctgcc
      301 cgatggcgaa tacgggctgc tcgccccgtt tctggacggc atggtggagg ccgcgagggg
      361 ccgcacccgc ttggtggacg gctacgagat cgcctattac cataacaagg acaccagcaa
      421 gttcgccgcc gcctaccaaa cgattcggga tgagctgctc cccatcgtcg ccaacccgaa
      481 ggaatatcgt cgccgcttct cggtgagctt cggtctctgg atggacttcg actcgactgc
      541 cataggctgg gacggcgaag acggcagcag gaatttctac acgcccgagg aatttgggag
      601 gagcgtccgc aaggctctcg aggttgccga cgaatacgtc tggatctaca gccagatccc
      661 tcgctggtgg tcgtccgagg ggaagacgat caaactgccc gagggctacg ctgacgccct
      721 gcgccaggcc cgccaagggt tggctccgtg acgcggcttc tggttcttgc gctgagcgcg
      781 cacaagccaa aaaggagcgc gactgaccgt gtcggtcccc acgcgtacac atggccggat
      841 acgacgggat tcggaagagg ggccgagggc agggacgggc cgaagcccgg gtgaagcgtt
      901 acgggaccag gacaagtttg ccgaattgct ccccggcgtc catgcggcgg tgagcttctg
      961 cggcctcccg cagggggaag acgcggtcca cgatcggctt gagtcggccc gtttcgtaga
     1021 gggacagcat ggcggcgaac tcccgcggac ttcccatggt ggtgcccagg atgctgagct
     1081 gattccagaa caccgtccgg atgctcacct cggttgtggg acccgtggtt gcgccaaagg
     1141 tgaccagcct gccgcccggt cggagcgccc gcaagtcctg ttcccaggtt gccttcccca
     1201 cgctgtcgat gatcacgtcc gcccctcccc ccgtgtgctt taggacgacc tcgtcccagg
     1261 cggtggaggt gtaattgatg gtgaggtccg cgcccagttc cctggcccga aggagcttat
     1321 catcgctcga ggatgtggcg atcaccgacg cccctgcctg ccgcgcgatc tgtagcgcga
     1381 aggtcgccac cccgcccccg atccccagga ccaccacccg ctctcccggc cgcacctgtc
     1441 cccgcgtgac aaccgcgcgc caggcggtga gcgaggccag cggcatggcc gccgcctcct
     1501 cgaagctgag gggggaaggc ttggggaaga cgttccccgc cgggaccacg atgtactcgg
     1561 cataggttcc tggatcggga cctcccagga tccggaaggc cgacgaggga cgatcctcac
     1621 gatctcccca gtccagcgag gggttgatca ccacctcctg tcctgaccgg agatggctca
     1681 ccgccggacc gacctcggcc acaagacccg cgccgtccga tccgggaatg agggggaggc
     1741 ggtccgtcat ctgccggccg agccggatcc acacgtcgcg gtggttcaag gcggcagcct
     1801 tcagatggac cagaacctct cccgggccgg gtcgtggggt gggagtttcc tcgaagcgaa
     1861 gctgctcggg tccgccgggt tggtgcagtc ggatggcgtg catgcgagga ctccttcagc
     1921 ggggccgaaa atgcgcgacg gccgggacca agtggcgggg agggggaggg tttccacaag
     1981 gtccagaccg tcgcgacagg aacgcggcat gcccatatgg tccggtcacg ccagcgatgt
     2041 ggcggactat acacacctcg atgggttctg ccaagttgag agtgaccgaa tgtatcggcc
     2101 agtggacaaa tcgggatgca gccgacaagg gcggcgtcgc cggaaagtgc tggacgaggc
     2161 ccctggttta ttggtatgtg cagggaggga accagcaggc atgatcgacc gacggaggga
     2221 tattgcatgg ggagcaatct gttcgacctc ggcggacgcg tggcggtggt gacgggtggc
     2281 ggcaccggct tggggaaagg aatcgcgacc ggtctggcgc aggccggtgc cttggtgatc
     2341 ctagccggcc ggcgacagga cgtggtggag caagccaccg cagagatccg ggccggcggg
     2401 ggaagggccg agggggtcac cctggacgtc acgcgtatcc aggaactgcc ggcgttcttc
     2461 gaggcggtga gcacgcgaca cggcgggctt gacatcctgg tgaacaacgc cggcaccaac
     2521 cggcggaacc catgtctgga atacacggag caggactggg acaccgtgat gaatctcaac
     2581 ctcaaggcgg tgttcttctg ttgccaggcg gcggcgcgga tcatgaagga gcgcggcggc
     2641 gggaagatca tcaacaccgc ctcgctggcg agcacggtca cctccacgaa ccaggccgcc
     2701 tacgcgccga gcaagggcgg ggtacggctg ctcacgatgc atcttgccct ggagttggcc
     2761 ccgtaccgca tcaacgtcaa cgccatcggg cccggctggt tccggacccc gctcaacgat
     2821 gacctgttcc gtaacgaggg ctgggcccgg ggcgtcaggg ctttgatccc ctgggggcga
     2881 accgggaccc ccgatgatct ggctgggacc gcggtctttc ttgcctcccg ggcctcggac
     2941 tatgtgaccg gacaggtgat ctacgtggac ggggggatgc tggcggggta caaagtccat
     3001 ccgatatcca cgcaggaggt ctagagctcg ataaccgctg gacatggtct tggattgttc
     3061 gcgttcggaa gttcgcccgg agaatggtcg actgttcccg gacaggcgaa cggcccaatg
     3121 ctctgggtgc tcatcccatg cgcatcttcg acgtcacgct gccgattcac cccgggatgc
     3181 cggtgtacga aggggatccc gaggtctcga tccggaaatg gttctctctg gatgggggcg
     3241 aggccgcgaa tgtctctgtg ctcagcctgg gctctcacac tggtacccac gtggacgcac
     3301 ctctgcactt ccgaaacggt gcgcccggcg tggatcgtct tcccctggat gtcctgatcg
     3361 gtccagcccg ggtatatgct ctggctctgg ccgatcacct tgacgccacc agcctgcggg
     3421 ggctgaacct cggggcgtac cccagggtcc tgttcaagac ccgcaactct gccgcctcac
     3481 gcgagggacg gtcgagcaag gacttcgtgg cgctcacggc tgatggagcg cgcctgctgg
     3541 tggaggtcgg tgtgcaactg gtcggcctgg acggcccctc cgcggactgc ttctccagca
     3601 cagatttccc cgtgcaccac acgctacttg acgccggggt catcattctg gagggcctcg
     3661 acctctctcc cgtccctccg ggggactacg agctcctgtg cctgcccttg atgatccggg
     3721 gaggtgatgg ggccccggcg cgggtcgtcc tgcgagaacg gacggaaggt cctgccccac
     3781 ctcttgccgg tgggatccag cggagaaccc acatctcacc cggtcgctga gggagggcac
     3841 gcgcatgcga cgaacaaaga tcgtgtgcac catcgggccg gccacatcct cgcccgagat
     3901 gattggacag ctcgtcgagg cgggtatgaa cgtcgcgcgc ctcaacttct cccacggcgc
     3961 ctgggaggag cacgccgggg tgatcgcgag tctccgccag atctccgcgc gcaagcacgt
     4021 cccggtggca atcctccagg atctgcaggg cccgaagatc cgtctgggga ctttccgggg
     4081 gggacgggcc agcctgcaac cgggggccga attcgtcatc accgtgcgcc cggtggaggg
     4141 cacggccgcc caggccagca cgacctacga ccaattgccc cgggatgtca agccgggcga
     4201 tcgcatcctg ctggcggatg ggacgatcga gctcaaggcc ctcgagagca ccgaggactg
     4261 ggtgcgctgc cgagtcgtgg tggggggaga gatcagcgat cacaagggca tcaacctccc
     4321 cggggtgtct gtcagcgcgc gttccatgac ggccaaggac cgagaggatc tccggttcgg
     4381 gc
//
LOCUS       DBNGZC010000455         1318 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_919740, whole genome shotgun sequence.
ACCESSION   DBNGZC010000455 DBNGZC010000000
VERSION     DBNGZC010000455.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1318)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_919740"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="AC16G0_08000"
     CDS             <1..724
                     /locus_tag="AC16G0_08000"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5099597.1"
                     /translation="TGGGRGIGATTALMMLAEGGGVGVVDVNPAHLSRVTETAQARGY
                     KLKTFLADVSKQDQVRTAVDAFVKEFGRIDILVNNAGMTIPRPFMEKTAEDWIRTLEV
                     NLIGVFLCAQAAAKHMLAQQSGKIINISSIRGVEHCGREGVMDYSAAKSGVINLTKTM
                     AKELAPHINVNTVAPGHTLTDLTAPLPDAVKKGMIEGAYLKRMAQPEDIAKAILFLAS
                     DDANFITGQMILVDGGFSLKAG"
     gene            747..941
                     /locus_tag="AC16G0_08005"
     CDS             747..941
                     /locus_tag="AC16G0_08005"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HIR5099598.1"
                     /translation="MPVITIRLLEGRSLEQKRELVKSISEAVSRIAKTPLEGVHVIFE
                     NVSRTDWGLGGELFADRNPR"
     gene            993..>1318
                     /locus_tag="AC16G0_08010"
     CDS             993..>1318
                     /locus_tag="AC16G0_08010"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5099599.1"
                     /translation="MRRKVAIAGFTMTLALAVSAWGGEKKVVHVWHTETEPQTVAAMQ
                     EIANDFEKLHPDVTIRQEGLAWGDLEAKLTAALAAGAPPEASHGQAFTCASFYHKGLL
                     RDQEDIA"
BASE COUNT          279 a          380 c          451 g          208 t
ORIGIN      
        1 caccggaggg ggacgtggga tcggcgccac caccgccctc atgatgctcg ccgagggcgg
       61 cggggtgggg gtcgtggatg tgaatccagc tcacctgagc agggtgacag agacggccca
      121 ggcccgcggg tacaagctca agacgtttct ggcggatgtg tccaagcagg atcaggtccg
      181 gaccgcagtg gatgcgtttg tcaaggagtt cgggcgaatc gatatcctgg tgaacaacgc
      241 ggggatgacc attcccaggc cgttcatgga aaagaccgcg gaggattgga tcaggacgct
      301 ggaggtcaac ctcatcggtg tctttctctg cgcccaggcg gcggcgaagc atatgctggc
      361 tcagcagtcg gggaagatca tcaacatctc ctccatccgc ggcgtcgagc actgcggccg
      421 cgagggggtc atggactaca gcgccgccaa gagtggggtg atcaatctca ccaagacgat
      481 ggccaaggag ctggccccgc acatcaacgt gaacacggtg gcaccaggcc acaccctgac
      541 ggacctcacg gccccgctgc ccgacgccgt caagaagggc atgatcgagg gcgcctatct
      601 gaagcgcatg gcgcagcccg aggacatcgc caaggccatt ctcttcctgg catctgacga
      661 tgccaacttc atcaccgggc agatgatcct agtggatgga ggattcagcc tgaaggcagg
      721 atagcgagag gaggtgaggc atcgccatgc ctgtcatcac gattcgtctc ctggaaggac
      781 gatccttgga gcagaagcgg gaattggtca agagcatcag cgaagcggtc tcacgcatcg
      841 ccaagacgcc cctggagggg gtgcacgtga tctttgaaaa cgtctcgcgg acggactggg
      901 ggcttggggg ggagttgttc gccgaccgga atccgcgctg accgatccag ggatatcgtc
      961 agcgggtcgc cgtgcttgga ggaggaagga tcatgaggcg aaaagtagcg atcgcgggtt
     1021 tcacgatgac tctggcattg gccgtatccg cctggggagg ggagaagaag gtcgtccacg
     1081 tctggcacac ggagaccgag ccgcagacag tcgcggccat gcaggagatc gccaacgact
     1141 tcgagaagct gcatccggac gtcaccatac gacaggaggg cctggcatgg ggcgacctgg
     1201 aggccaagct gacggcggcc ctagccgcgg gcgcgccccc ggaggccagc cacggccagg
     1261 cattcacctg cgcctcgttc taccacaagg gactcctgcg tgaccaggag gacatcgc
//
LOCUS       DBNGZC010000456         2308 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_233938, whole genome shotgun sequence.
ACCESSION   DBNGZC010000456 DBNGZC010000000
VERSION     DBNGZC010000456.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_233938"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1174)
                     /gene="pdxA"
                     /locus_tag="AC16G0_08015"
                     /pseudo
     CDS             complement(158..1174)
                     /gene="pdxA"
                     /locus_tag="AC16G0_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015512918.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
     gene            complement(1342..2169)
                     /locus_tag="AC16G0_08020"
     CDS             complement(1342..2169)
                     /locus_tag="AC16G0_08020"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR5099600.1"
                     /translation="MQLSLLASTPDIAATGYMVNLLLGAPQALAGEARELGYDGIEFF
                     PGPPGTVSVAEMNEALRSFDLTLTAVNSGRIVAEGLTLLHPEPTIRGRAQARLKDLLD
                     FAGHFGAPVTMAGVKGSLPAGAPPGRGEALAQEIFDSLAKFAIDAGSVLLLTPTDEAD
                     SNLICTVAEAMAWVQRVNRPGFGMMLDTHQLARKEVSVIEGLRAGDGLIRHLHLYDPG
                     RRPPGTRKENRLDWPAIMATLRDIDYDGAASVCLAPTGDRRAQAQHTAAYLRSLMAS"
BASE COUNT          385 a          747 c          731 g          445 t
ORIGIN      
        1 gacctgtaag ccttcggctc gccccattgc accgcctcgg ccccctcctc agtcgtcatt
       61 ccggcggaag ccggaatcca gtgtgccgag aactgtggac ccggctttgg tcggggggac
      121 gacctaggat ggcaccctaa ggcgaatcgc gagcggccta tgcgaagagg cctgctttcc
      181 gtccctgggc gagcttcacg gccgcgtcga tggcatccag catgctctgg tgggcggcgg
      241 tgcccttccc ggccttgccg aaagccgtcc cgtgatccac cgaggtccgg atgattggca
      301 gacccaaggt cacgttgacc ccgctcaccg acgtccactt ctgtgtggcc ttatcgaagg
      361 tgaagcctag cgtcttcagg ggtacgtgtc cctggtcgtg gtacatgcag accacgatgt
      421 catacagacc gccccgggcc cgaccgaaca cggtgtcggg tgcgatcggg ccgtccacct
      481 tgatgccctc ggccctggcc tcctcgatgg ccgggatgat ctccttgacc tcctcgttgc
      541 cgaacagacc gtgctcgccg gcgtgagggt tcagaccggc gacggcgatc ttgggctcgg
      601 cgatgcctag ggccttgagg gcccgattcg ccagacggat gacggtcagg acccgtggtt
      661 tcttcgtgcg gttgatggct tcctgcaagg aaacgtgggt ggagacgtga accacccgca
      721 gatcctcccc caggagcatc atggcatagt ccttggtgtt ggtcagggct ccgaagatct
      781 ccgtgtgacc cgggtgcggg cagccggctt ggttgatgga ctccttgtgg atcggcccgg
      841 tcacgacggc gtcgatctcc ttggccaggg ccagggcgat gcccttctcg atctactcca
      901 ccgaggcctt gccgcacatg gcgctcacct tgccgtactg aagcttggcc atgtcaacgt
      961 tggccaggtc catgacgtcc agggtcccgt gcgtgaatcg tgcctcggcc accttggcga
     1021 tcgggtgtag cgtgagggtc agcttggtta tgcggagcgc gtcagccatc gctttcttgt
     1081 cgccgatgat gaagggccgc gacttggcat aaacagtggg gtcggccaga gccttggctg
     1141 cgatctccgg cccgatgctg gcaggatccc ccatggtgat gccgagaatt ggtcgatcca
     1201 tgatcacgtg cctcctcttg acctccagcg gtcagtggtc acccgcccga gcctctcgtg
     1261 atacctgtct ctatcggctg cgggctttgt agcatacctg ggcgcggccg tccgtcccca
     1321 gcttgaacgt gaacggttca ctcacgaagc catgagcgat cgaagatagg ccgcggtgtg
     1381 ctgcgcctgg gcccgacgat cgccggtggg ggccagacac acggaggcgg caccgtcgta
     1441 gtcgatgtcg cgtagggtgg ccatgatggc cggccagtcc aggcgatttt cctttctggt
     1501 tccgggcggg cgacgacctg gatcgtacag atggaggtgc ctgatcaacc cgtcccctgc
     1561 gcgcaacccc tcgatcaccg acacctcctt gcgcgcgagc tgatgcgtgt caagcatcat
     1621 gccgaagcca ggacggttca cgcgctggac ccacgccatc gcctcggcga cggtgcagat
     1681 gaggttgctg tcggcctcgt cggtcggggt cagcaataga accgagcctg catcaatcgc
     1741 aaatttggcc aggctgtcga atatctcttg cgccagagcc tccccccttc cgggaggcgc
     1801 cccggcaggc aggctgccct tcaccccggc catcgtcacg ggggcgccaa agtggccggc
     1861 gaagtccaaa aggtccttga ggcgtgcctg ggcgcgaccc cggatcgtgg gctcaggatg
     1921 cagcagggtg aggccctcgg ccacgatacg cccactgttc accgcggtca gggtgaggtc
     1981 gaagctccgc aacgcctcgt tcatctcggc cacactgacg gtcccgggag gacccgggaa
     2041 gaactcgatg ccgtcgtagc cgagttccct cgcctctccg gccagggcct gcggcgcgcc
     2101 caggagcaga ttcaccatgt accccgtggc cgcgatgtcc ggcgtgctgg ccagcaagct
     2161 caactgcatt ggagaacaag aactcctttt gattatccgc agattacgca gattccgcag
     2221 attccctgag ggacatcacc aatcgtcgat gttcgagcga ccgcgcgccg aaattgaaca
     2281 aaaggcccac agaatggcca ctggcctt
//
LOCUS       DBNGZC010000457         3426 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_526970, whole genome shotgun sequence.
ACCESSION   DBNGZC010000457 DBNGZC010000000
VERSION     DBNGZC010000457.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_526970"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..1452)
                     /locus_tag="AC16G0_08025"
     CDS             complement(214..1452)
                     /locus_tag="AC16G0_08025"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015112 - nitrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5099601.1"
                     /translation="MRRPDAIWLAAVCVCEMATMLMFLNYTAILPILQNEWGMRHGQA
                     GLIFSAYQACYLLAVLVLASLTDRMDTRAIYLASAVWAGLAGLTFPFFAQDFRSAAIL
                     RALAGIGLAGTYVPGMRLVADRFPSERRGFAIAVYASAFGLGSALSLALTGSLTRFSG
                     WRLAMGVTSLGPLLAAGLASVVLSPAPPPSTRSPRLLDARVLKNRPALLMIAGYCAHN
                     WELFGMRSWLPPFFAAIFTRRGTSVVDASSLGAAIASGILLVGSVSNIGGGWLSDRWG
                     RVQVIILSQTVSAACSFIIGWFFAAPLWVILGLAALYGVFVSSESGALSTSVTELAEP
                     SALGATLAVQSCLGFLAAGMAPASVGFLLDQLQPITGGTAAASPWQWGPAFGILGLGV
                     LFGPLSMLALRRAQRTSRHR"
     gene            complement(1452..2174)
                     /locus_tag="AC16G0_08030"
     CDS             complement(1452..2174)
                     /locus_tag="AC16G0_08030"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR5099602.1"
                     /translation="MRIDGVIFDLDGTLADTLPVCFVAFRRTMEAFTDRRFTDEEIMS
                     HFGPSEEGIFQRLVPDRWEACLRVYLEIYEQEHLRAARLFPGIEEALRRLRRRGMPLA
                     IVTGKGLHSAAISIRHLGLARYFDVLETGGPEGSIKPQAIRRVLARWAVLPERVAYVG
                     DAASDMEEAKAAGLIPLGAAWDPRASVQVGNGRDPLLLFPSVESFTDWIEVAVEFSAC
                     AGEAEASPAPPQKVPPTQNPAG"
     gene            2565..2846
                     /gene="rpsU"
                     /locus_tag="AC16G0_08035"
     CDS             2565..2846
                     /gene="rpsU"
                     /locus_tag="AC16G0_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR5099603.1"
                     /translation="MAFRDGQNGDNYPPAGGNAPRHRPLEVTVGDRGVEGAIRLFKRL
                     VLRDGILRDLKRRAHYEKPGDRRRRKKREAARRLRKRLVRALQRGDQNE"
     gene            2967..3293
                     /locus_tag="AC16G0_08040"
     CDS             2967..3293
                     /locus_tag="AC16G0_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR5099604.1"
                     /translation="MGTRLYVGNLPFDTDETQLRTLFSEGGRQVTEVKIITDRDTGRP
                     RGFAFVEMGSQADADAAVNTLNGREFGGRALTVNEAREQAPRRGGGFGGGGGRGGGGG
                     GGRRGY"
BASE COUNT          657 a         1052 c         1195 g          522 t
ORIGIN      
        1 tcgagcctcc ggttccgagc agggtcggtc tctcgagctg cgagcttcgc gagcgcggga
       61 agcggcggac cgaccgtgtg ggcatgaagc tctgagtatc tctgctgccc ggggaaggcc
      121 gagcccctga ggtttctacc ggcagggctg gagcgcatgg ggaatcgcgc gacgccccta
      181 ccggcctgct cgcgcctcag acgcaccgcc tcgtcatcga tgtctcgaag tcctctgagc
      241 ccggcgcagg gcgagcatgc tcaaggggcc gaagagcacg cccaacccta gaatgccgaa
      301 ggccggtccc cactgccagg gcgaggcggc tgcagttccg ccggtgatgg gctgaagctg
      361 gtccagaaga aagcccaccg aggcgggcgc catgcccgcc gccaggaatc cgagacagga
      421 ttgaacggcg agggttgcgc ccagggcgga gggttctgcc agctcggtca cgctcgtgga
      481 cagggcgccg gattcggagc tgacaaagac accatacagg gccgcaagtc ccagtatcac
      541 ccagagtgga gccgcaaaga accagccgat gatgaatgag caggcggcgc tcacggtctg
      601 ggagaggatg atcacctgga cccgtcccca gcgatccgag agccagcccc cgccgatgtt
      661 tgagacagac cctaccagca ggatacccga ggcaatcgcc gcccccaggc tgcttgcgtc
      721 caccacgctg gtcccgcgcc gggtgaagat cgcggcgaag aacggcggga gccaggagcg
      781 catgccgaag agttcccagt tgtgggcgca gtagcccgcg atcatgagca gagccggacg
      841 attcttgagg acccgggcat ccaggaggcg tgggctccgc gtggaaggcg gaggggcggg
      901 ggagaggacg acggaagcca gcccggcggc gaggagaggt cccagggagg tgacacccat
      961 ggcgaggcgc cagccagaga accgggtcaa ggagccggtg agcgccaggg acaaggcgga
     1021 gccgaggcca aaggcgctgg cataaacagc gatggcaaat ccccggcgct ccgaggggaa
     1081 tcggtcggcg acgagccgca tccccggaac ataggttccg gcgaggccaa tcccggccag
     1141 ggcgcggagg atggcggccg aacggaaatc ctgggcaaag aaggggaagg tgagaccggc
     1201 tagccccgcc cagacggccg aggcaagata gatggcgcgg gtgtccatgc ggtcggtgag
     1261 actggccagg acgaggaccg ccaggagata acacgcctgg taggcggaga agatcaggcc
     1321 cgcctgcccg tgacgcatcc cccactcgtt ctggaggatc ggaaggatcg ccgtgtaatt
     1381 gaggaacatc agcatggtgg ccatctcgca cacgcatacg gccgcgagcc agatggcatc
     1441 cgggcgtcgc atcagccggc agggttctgt gtggggggaa ccttctgtgg gggtgccggg
     1501 gaggcctcgg cctcgcctgc gcaggcagag aactccacgg caacctctat ccaatccgtg
     1561 aagctttcaa cgctggggaa gagcagcagc ggatcccgac cgtttccgac ctgaacgctt
     1621 gccctcgggt cccaggcggc tcccagcggg atcaggccgg ccgctttggc ctcttccata
     1681 tcggaggcgg cgtccccgac atacgccacc cgctccggca ggacagccca cctcgccagg
     1741 acccttcgaa tagcttgtgg cttgatactc ccctcgggac caccggtctc caggacgtcg
     1801 aagtagcggg ccaagccgag atggcggatg gagatggccg cgctgtggag ccctttccct
     1861 gtcacgatgg ccagaggcat cccgcgcctc ctcagccgtc gcaatgcctc ctcaatccca
     1921 ggaaagagtc gagcggcccg cagatgctcc tgctcgtata tctccaaata gacgcggaga
     1981 caggcctccc atcggtcggg caccaggcgc tggaatatgc cctcctcgct cggcccgaag
     2041 tgggacatga tctcctcatc cgtgaaccgt cggtccgtga acgcctccat ggtgcgtcgg
     2101 aaggcgacga agcaaacggg taacgtgtcg gcaagggttc cgtccaaatc aaagatgacc
     2161 ccgtctatcc gcatggtgac tccacttttg tcctgcggcg ctgtgccaga ttcagcctga
     2221 aggcctctga cgctattctg cttctcgcgt caatgtcaag ggctatgcgt tcgatagacc
     2281 gagtcatcgc ctggtcagcc gaccgtcggg ctggcggcct tttgccacgt cggccgatcg
     2341 catggatgat gccggtcaga tccaaaagag cgaactatct cggccaattt ccggggcgat
     2401 tctacttgac aaggaggggg aatgcccgta gagtccggga caaggacggt tcagacggtt
     2461 tcgcgttttc gtacaaacgc cgcgaggacc ctagccttgc ggcgttttgc ttttctcgtg
     2521 gcgtggccct cggggaccga gcacagtctc ggcggggagg atgcatggcc ttccgggatg
     2581 gacagaatgg ggataattat cccccggcgg gtgggaacgc cccacgccac cgtccattgg
     2641 aagtcaccgt tggggatcgg ggggtggagg gcgccatccg actgttcaag cggttggtcc
     2701 tccgggatgg catcctccgc gatctcaagc ggcgagccca ttacgagaag cccggcgacc
     2761 gtcgccgtcg gaagaagcga gaggcggcgc gtcgccttcg gaaacgactc gtgcgtgccc
     2821 tgcagcgagg ggatcagaac gagtgacgga gaagggtacc ctggacggtt cgggacgagc
     2881 gggcccggga ccggacgatt cagctacgac gaccgctcgg gatgggaaac ggtcccgcaa
     2941 tggcggggcg aagggggttg gggacgatgg ggacgaggct gtacgttggg aatctgccat
     3001 ttgacacgga cgaaacacaa ctccgaactt tgttctcgga gggcgggcgc caggtcaccg
     3061 aggtgaagat catcaccgac cgagacacgg gtcgtccccg cgggttcgcc ttcgtggaaa
     3121 tgggcagcca ggcagatgcg gatgcggccg tcaacacgtt gaacggacgg gaattcggcg
     3181 gacgggcact cacggtcaac gaggcccgcg agcaggctcc tcgccgtggc ggaggattcg
     3241 ggggcggcgg cggtcgcggc ggtggtggcg gcggaggacg gcgcggctac tgagcccagc
     3301 cgagagcctt cggcccaccg ctcctctttc tgccgacggt cattcctcgc ggtcaggaca
     3361 tcggataccg ggcggatctc gtacggcatg gccttcgcgc cacgtcggca gggtccgccc
     3421 ggattt
//
LOCUS       DBNGZC010000458         2157 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2679627, whole genome shotgun sequence.
ACCESSION   DBNGZC010000458 DBNGZC010000000
VERSION     DBNGZC010000458.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2679627"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="AC16G0_08045"
     CDS             <1..834
                     /locus_tag="AC16G0_08045"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099605.1"
                     /translation="MAGDPVLLLVPVDSSPEFIERMRVQWGFADPLWVQYGRFIGRAA
                     LGDFGTSLKYNVPALTLVLERLPATGLLTLVSMIFAVVLGVPLGFLAAVRSGRLADRL
                     STVGSVLGQSIPSFWLGLLLILVCSVMLGVLPTGGMGTAKQLILPGVTLGLYSLGRVV
                     KLTRSGVLEVLRADFIRTARAKGLRERVVLYRHVLKNAAIPILTIIGLQMGGLIGGAI
                     VVETIFAWPGLGRLIVQSIQFRDFPTALAAVFFTAVSVSVINLVVDVLYGLLNPRIVY
                     E"
     gene            869..1747
                     /locus_tag="AC16G0_08050"
     CDS             869..1747
                     /locus_tag="AC16G0_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099606.1"
                     /translation="MDQAARTEGNRRSPVRRLRIRLDGGAAWGIGAALFLMLEAFLAV
                     GAPLLTGYDPLKGDLDRRLLAPGAAGHLLGTDQLGRDLQARMLYGARVSLVVGAVAVA
                     VAGTVGVAAGLVMAYRGGWLDSVGSRLVDVQLSFPFMALALSIAAVLGASLTGIVATL
                     AISSWVLYARLVRGETLVMKQREFVEAARTLGAPWWRILARHVLPNVVGPAIALASLE
                     AGRLIVAESSLSFLGYGIQPPIPAWGNMLAEGKDYMARAWWLTVLPGLAVSLTCLSTT
                     LLGDWLTEVLEPRRSP"
     gene            1817..>2157
                     /locus_tag="AC16G0_08055"
     CDS             1817..>2157
                     /locus_tag="AC16G0_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013486.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HIR5099607.1"
                     /translation="MSASKLKRVAAALKRSGMDALVLLPGPNLYYTLGINKMLRKRPI
                     IYVVFSDESLAASLPLLEVPDFRTRWPEAQVISWTDAEGPDSAAKRLGAIVRERSQAS
                     TPRVAAEYFTLR"
BASE COUNT          289 a          699 c          751 g          418 t
ORIGIN      
        1 atggcgggag atccggtgct gctcctggtg ccggtggact cctcacccga gttcatcgaa
       61 cgcatgcggg tgcagtgggg gttcgccgat cccctgtggg tgcagtacgg gcgcttcatc
      121 ggacgcgcgg cgctgggcga tttcggcacc tcgctgaaat acaacgtgcc ggcgctcacc
      181 ttggtcctgg aacgtctgcc ggcgacgggt ctgctcaccc tcgtgtccat gatcttcgcc
      241 gtcgtcctgg gggtgccgct gggattcctg gcggcagtcc ggagcgggcg gctcgccgac
      301 aggctgtcca ccgtcggctc ggtgctggga cagtcgatcc cgagcttctg gctgggactc
      361 ctgctcatcc tggtgtgctc cgtgatgctc ggcgtcctgc caaccggggg catgggtacc
      421 gccaagcagc tcatcctgcc cggcgtcacc ctgggactct attcgctggg tcgggtggtc
      481 aagctcaccc ggtccggcgt gctggaggtg ctgcgtgcgg atttcatccg cacggctcgg
      541 gccaaggggc tgagggagcg ggtggtgctg taccgccacg tgctcaagaa cgccgccatc
      601 ccgatcctga ccatcatcgg cctgcagatg ggagggctta tcgggggcgc gatcgtggtc
      661 gagaccatct tcgcctggcc cggtctgggc cggctgatcg tgcaatcgat ccagtttcgt
      721 gacttcccca cggcgttggc ggcggtcttc ttcaccgcgg tctccgtctc ggtgatcaat
      781 ctcgtcgtgg atgtgctcta cgggctcctg aacccccgga tcgtgtatga gtgactcgcg
      841 ggaatcctgg agcggcgagg gcattccaat ggatcaggcc gcccgtacgg agggcaaccg
      901 acgttcgccc gtccgtcggc tgaggattcg cctggatggc ggcgcggcct ggggcatcgg
      961 cgccgccctc ttcttgatgc tcgaagcgtt cctggccgtc ggcgcaccgc tcctgaccgg
     1021 atacgacccg ttgaagggag accttgaccg gcggctgctg gcgccgggtg ccgcgggaca
     1081 cctgctggga accgatcagc tgggccggga tcttcaggcg cgcatgctgt acggtgcgcg
     1141 ggtctccctc gtcgtcgggg cggtcgccgt ggccgtggca ggcacggtgg gggtggcggc
     1201 cggactggtc atggcctacc ggggaggatg gctggacagc gtcggctcgc gcctggtgga
     1261 cgtccagctc agtttcccct tcatggcgct ggcgctgagc atcgccgccg tgctgggcgc
     1321 gagcctgacg ggcatcgtgg ccaccctcgc catatcctcc tgggtgcttt acgccagact
     1381 cgttcgcggt gagaccctgg tcatgaaaca acgggagttc gtcgaggcgg cgcgcacgct
     1441 gggggcaccc tggtggcgta tcctggcccg ccacgtcctt cccaacgtcg tcggacccgc
     1501 catcgccctg gcatccctgg aggcgggacg gctcatcgtc gccgagtcct ccctgagctt
     1561 cctgggctat ggcatccagc cgccgatccc ggcctggggg aacatgctgg ccgaggggaa
     1621 ggactacatg gcgcgggcct ggtggctgac cgtgttgccc ggcctggcgg tgtccctcac
     1681 ctgtttgtcc acgacccttc tgggcgactg gctgaccgag gtcctggagc cccgccgcag
     1741 tccctgaacc cagcgggcgg gatcgacctg ccctggtggc aggtttggcg aaacgatttc
     1801 tcgggaggat ttcctcatgt cggcatccaa gctgaagcga gtggcggccg ccctcaagag
     1861 gtcgggtatg gatgccctgg tgctgctgcc agggccaaat ctctattata ccctcggcat
     1921 caacaagatg ctccggaagc ggccgatcat ctatgtggtg ttttccgacg agagcctggc
     1981 ggcatctctc cctctcctgg aagtgccgga ctttcggacc cgatggcccg aggcgcaagt
     2041 catctcctgg accgacgccg aggggccgga ttcggcggcg aagaggctgg gagccatcgt
     2101 ccgggaacga tcccaggcca gcacgccccg ggtcgcggca gagtacttca ccttgcg
//
LOCUS       DBNGZC010000459         1891 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1701501, whole genome shotgun sequence.
ACCESSION   DBNGZC010000459 DBNGZC010000000
VERSION     DBNGZC010000459.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1701501"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..249
                     /locus_tag="AC16G0_08060"
     CDS             70..249
                     /locus_tag="AC16G0_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099608.1"
                     /translation="MKEDEPSLGLASRGRIPPEIDFGDAGAPAKATIRNGRKYFYRID
                     SRLGLWLIIDGVEDG"
     gene            complement(601..858)
                     /locus_tag="AC16G0_08065"
     CDS             complement(601..858)
                     /locus_tag="AC16G0_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099609.1"
                     /translation="MTTIVMYRDDQPPINHFPKRIISPTRSGPCCLSGMEDLGVVWRQ
                     GQWEYAYRRCRTCGFTVRGIGRYVLDDDEIAKFQKRFAAIP"
     gene            complement(1310..>1891)
                     /locus_tag="AC16G0_08070"
     CDS             complement(1310..>1891)
                     /locus_tag="AC16G0_08070"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR5099610.1"
                     /translation="FPVRVPVVAEPDAPAGYPTPGETQNLSRSGVLLRVERAERRGVP
                     VRVTLPLRPHVSATLAGTVVWTRPQADRPGWEQGIQFHSEVPGALVLALTDETLPPRG
                     TRPARGAAGVTAMHAQPIQGLLFGFVLGLALWLVAGLVSLRAVGCLPYVPTSLWCVLG
                     FLVICTTVEVTRTFLRGRAAMVPSPHIHRRSVG"
BASE COUNT          353 a          575 c          649 g          314 t
ORIGIN      
        1 ataccagcga gagagcctcc cgatttattg tgcagcaggg gggcgatgtg agacgggccg
       61 gtcgccctaa tgaaggaaga tgaaccctct ctaggcctgg cgagccgagg gcggatccct
      121 ccggagatcg atttcgggga cgccggggcc ccggcgaagg caacgatccg gaacgggagg
      181 aaatacttct acaggatcga tagcaggttg gggctctggc tcatcatcga tggcgtcgag
      241 gacggatagc ctggtgtccg gccccggagc atgctctatc cttccagctc atgtcgagat
      301 catgtgtgtc agcactccga ggaaagggaa ggggcaacag agacgggctc gatggctgcg
      361 gggcaccagc ctcggccgcc agggccatcc ggccggcgac tgttagaccg gcgtatgcgc
      421 gcgcgatctc cgagagtgac cggctggttt ggagaggagt tcgatgcgct cgggtgcgac
      481 cacccggagg ttgaacgtgc ggctcaaatt ttctggcggg gggtaggatt gcgggtgatg
      541 ctgtcaacga taaggaaatt gctcagagaa ggcatgggct cgacggccgc aattcacctg
      601 ctaggggatg gcagcaaagc gcttctggaa tttggcgatt tcatcatcat ctagcacgta
      661 gcgcccaatt ccccgcaccg tgaatccgca ggtccgacag cgccggtacg cgtactccca
      721 ctgcccctgg cgccagacga ccccaaggtc ttccatcccg gaaaggcagc aggggccaga
      781 acgcgtgggc gagataatgc gcttgggaaa gtgattgatc ggcggttgat cgtcccggta
      841 catcacaatc gtggtcatcg ctgactcctc tccaaggctc gcggatgtgg ttcaacctac
      901 ttgcctcggg gacctcccag ggtctcaccc ttcgagtttc ggttttgcac gggcggtgcc
      961 agcggccgtt cggccccggt acccctacgg caactccgcg acccagaagc acctgtgggg
     1021 cggccacgac tgaggcgagc gcgggtttcg ccgtgggcgt cggcggttcc ccgcccgagg
     1081 cggggtgcag cggtttgcat ccgtggcagt ccctgtgcaa tattcttcac gcgagccgca
     1141 gtcggggttg gctcggaagg ggtagccgcg cgggatactc gggtaaagcg ttggagaaag
     1201 aaggtccagt cgcgccacac acgctcggcc ggtgggggtg cgagggagat gcacccgaag
     1261 cacgtgggtg aagccgtgac gtgtaggtat tcccactcga cgcgcgctgc taccctacgc
     1321 tacgtcggtg aatgtgggga gaagggacca tggctgcacg gccacgaagg aatgtccgtg
     1381 tcacctcgac cgtcgtgcaa atcacgagga agcccaatac gcaccacagg gaggtcggca
     1441 cgtaaggcag gcagcccact gcgcggagtg aaaccagtcc ggctaccaac cacagggcaa
     1501 gcccaagcac aaagccgaac agaagccctt ggattggctg ggcatgcatg gcggttaccc
     1561 ctgcggcgcc acgggcgggt cgcgttcccc gtggcggtag cgtttcatcg gtaagcgcga
     1621 gaacgagggc gccagggacc tccgagtgga attggattcc ctgctcccac cccggtcggt
     1681 cagcctgcgg ccgggtccag acgaccgttc ccgcgagggt ggccgagacg tgagggcgca
     1741 gcggcagcgt cacccgcacc gggaccccgc gcctctccgc ccgctccacc cgcagcagca
     1801 caccgctccg gctcaggttc tgggtctccc ctggggtggg atacccggcg ggcgcgtccg
     1861 gctctgccac gaccgggact cgcacgggga a
//
LOCUS       DBNGZC010000460         1588 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1112083, whole genome shotgun sequence.
ACCESSION   DBNGZC010000460 DBNGZC010000000
VERSION     DBNGZC010000460.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1112083"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="AC16G0_08075"
     CDS             complement(<1..304)
                     /locus_tag="AC16G0_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5099611.1"
                     /translation="MAKILVVDDETLLRTILSDALESAGHTVTVAVDGKSALACAKDD
                     HPDCILLDIMMPGLDGYQTCEALKADPELARIPVLLVSATTDLRVVDRAEKVGAAGV"
     gene            complement(470..>1588)
                     /locus_tag="AC16G0_08080"
     CDS             complement(470..>1588)
                     /locus_tag="AC16G0_08080"
                     /inference="COORDINATES: protein motif:HMM:NF013206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR5099612.1"
                     /translation="ERIVAFITTSPVEDASDTPHTGLLSYEDMAEWHAAVEDPVASPY
                     GDLEVHKCPPWTQGPVFLQQLAILEGYDLAALGHGSPEYLHLLIESAKLAFADREAYY
                     GDPLFDKVPLDILLSKEYTAKRRSLIGADASREMRPGDVGQGIPAYVTFDVAEDNRRA
                     LQVAARSIRDLGLGHAHVGDTTHLDAVDAEGNMVAATPSGGWIGSSPVIKGLGFPLGT
                     RGQMFYLNPERPNALAPRKRPRATLTPSLVTKHGEPFMVFGTPGGDGQDQWTLQFFLN
                     VAVFGMNLQEALDAPTIHSVHFPSSFYPRPAYPGRVEVEGRIPAEVIASLENRGHEVK
                     VIDGWANGKVMGIRRDPERGVIAGAVSPRRTIGYALGW"
BASE COUNT          265 a          533 c          500 g          290 t
ORIGIN      
        1 agacgccggc ggctccaacc ttctcggccc gatccacgac gcggagatcg gtggttgctg
       61 aaacgagcag gaccgggatc cgcgccagct cggggtccgc cttcaaggcc tcgcatgtct
      121 gatagccgtc gaggcccggc atcatgatgt ccagaaggat gcagtccgga tggtcgtctt
      181 ttgcgcaggc cagggcggac ttaccatcta ccgccacggt tacagtatga cctgccgact
      241 cgagtgcatc gctgaggatg gtgcgaagca acgtctcgtc atctacgacc aggattttcg
      301 ccataccgag acgaccctca tggtatgcgg gaggtccgct ccaaggcgag cctacctacc
      361 gcccttgtcc ctgtcaaact ctttgtctag gctttgtttg cacgttcccc gccatctgtt
      421 ccaatggagg aacttccgcc tgtggctgac ctgccccggc ggcagccgtt caccacccca
      481 gggcgtaccc gatggttcgc cggggggaga ccgcacccgc gatcacgccc cgttccggat
      541 cccggcggat tcccatcacc ttgccgttgg cccagccgtc tatcaccttg acttcatgcc
      601 cccggttttc caagctcgcg atcacctctg ctgggatgcg tccctccacc tccacccggc
      661 cgggatacgc cgggcgcgga tagaaggagg aggggaagtg cacggagtgg atggtcggcg
      721 cgtccagggc ctcctgcaga ttcatgccga acaccgccac gttcaggaag aactgcagcg
      781 tccactgatc ttgcccgtcg ccacccggcg taccgaaaac catgaacggc tcaccgtgct
      841 tggtcacgag ggacggcgtg agcgtggccc gcgggcgttt ccggggggcc agggcgttgg
      901 gtcgctcggg attgaggtag aacatctgcc cccgggtccc caagggaaag cccaggcctt
      961 tgatgaccgg tgaggaaccg atccacccgc cgctgggggt ggcggcgacc atgttgccct
     1021 cggcgtccac ggcgtccagg tgggttgtgt ccccgacgtg ggcgtggccc agccccaggt
     1081 cccggataga acgggccgcg acctgcagcg cccgccgatt gtcctccgcc acgtcgaagg
     1141 tcacatacgc gggaatgccc tgcccgacat cgccgggccg catctcccgc gacgcgtccg
     1201 ccccgatgag gctcctccgc ttcgccgtgt attccttcga gagcaggata tccaggggaa
     1261 ccttgtcgaa gagcggatct ccgtagtagg cctcgcggtc ggcgaaggcc agcttggcgg
     1321 actcgatcaa gaggtggaga tactctgggg agccgtgtcc caaggctgcg agatcgtaac
     1381 cctccaggat cgccaactgc tgcaggaata ccggcccttg cgtccagggc gggcacttgt
     1441 ggacctcgag gtctccgtac ggcgacgcca ccgggtcttc caccgccgca tgccattccg
     1501 ccatgtcctc atacgagagc aggccggtgt ggggagtgtc gctagcgtct tccaccgggc
     1561 tggtcgtgat gaacgccacg atgcgttc
//
LOCUS       DBNGZC010000461         1730 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2483793, whole genome shotgun sequence.
ACCESSION   DBNGZC010000461 DBNGZC010000000
VERSION     DBNGZC010000461.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2483793"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1514)
                     /locus_tag="AC16G0_08085"
     CDS             complement(135..1514)
                     /locus_tag="AC16G0_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR5099613.1"
                     /translation="MGTGAPTPVVIPAHCAPTKAPCPTCGKHGRRKRTFTRTVRTVAY
                     KTVAFLEITCGEYGARCDCCTTFRNAPEGVLPRALYDDKVRDLVLDRILKDGMSIERT
                     LESLSREFLLDLSTGFVYDVLYDHAKQLDMAAHRRTVLALFSGTLCVDELHLGRFTLL
                     LATDPLSDLPVAFALVAANDQGHMRRFLGNLKNWGLDPEVVVTDGSNLYPTVLAELWP
                     DADHQLCVFHVIKDINKLTLDAVRRMRTAMSRRGKAGRKKKRGRKGAKSKAAVRRGMT
                     VKEKAHFVFKRRHLIVKRREGLTEAERDDLTRMLEYLPELATLRRFADRIYWLFDAPK
                     DLHQAGCRRAAIVRDPAFRAVPELVKAMEQIDAEKFPKLMVYLNNPVSRRVRTNNHVE
                     RTNRMFRFLEKVRYKWRRRRTLVRFVVLTLDGIWRERTIAQAKVTDRSKPTGPATTES
                     EKRKRRQAA"
BASE COUNT          275 a          573 c          553 g          329 t
ORIGIN      
        1 aaatcgcgta caatggagcc ctctgatgcg tccccctgga atccgtcgtt atccgtacac
       61 ggagggctcc catgtcctgg tcccacctca agggagggaa agggggagac ttctcgctga
      121 acaccaccgc acactcacgc ggcctggcgt cgttttctct tctcgctctc cgtcgtggct
      181 ggccctgtcg gctttgagcg atccgtcacc ttcgcctggg cgatggttcg ttccctccaa
      241 atgccgtcca gcgtcaacac cacgaatctc accaacgtcc gccgtcgacg ccacttgtac
      301 cgcaccttct ccaggaaccg gaacatccgg ttggtccgct ccacgtggtt gttggtccgc
      361 acccgtcggc tcaccgggtt gttcaagtac accatcagct tggggaactt ctccgcgtcg
      421 atttgctcca tcgccttgac cagctcgggc acggcccgga acgccgggtc gcgcacgatc
      481 gcggcccggc ggcagcccgc ctggtggaga tccttgggcg catcgaacag ccagtagatc
      541 cggtcggcga accgccgcaa ggtcgccaac tcgggcaggt actccagcat ccgggtcaag
      601 tcgtcgcgct ccgcctcggt gaggccctcc cgccgcttca cgatcaggtg acggcgcttg
      661 aacacgaagt gcgccttctc cttgaccgtc atgccccggc ggacggcggc cttggacttg
      721 gcccccttgc ggccccgctt cttcttgcgc ccggccttgc cgcgccggct catcgccgtc
      781 cgcatccgcc gcacggcgtc gagggtcagc ttgttgatgt ccttgatgac gtgaaagacg
      841 cacagctgat ggtccgcgtc gggccacaat tcggccagca ccgtggggta caggttcgag
      901 ccgtcggtca ccacgacctc ggggtccagt ccccagttct tgaggttccc caggaaccgc
      961 cgcatgtggc cctggtcgtt ggcggcgacc agggcaaagg ccacgggcag gtcggacagc
     1021 gggtcggtag ccaagagcaa cgtgaagcgt cccaggtgca gttcgtcgac gcagagcgtg
     1081 ccgctgaaga gtgccaggac cgtgcggcga tgcgcggcca tgtcgagttg cttggcatgg
     1141 tcgtagagca cgtcatagac gaaccccgtg gagaggtcga ggaggaactc gcggctcagc
     1201 gactccaagg tccgctcgat gctcatcccg tccttgagga tgcgatcgag gaccaggtct
     1261 cggaccttgt cgtcgtagag ggccctgggg agcaccccct cgggggcatt gcggaaggtc
     1321 gtgcagcagt cgcaccgggc gccgtactcg ccgcaggtga tctccaggaa ggcgacggtc
     1381 ttgtaggcga cggtgcggac ggtgcgagtg aacgtccgct tgcggcggcc gtgtttgccg
     1441 cacgtcgggc acggggcctt cgtgggggcg cagtgagccg gtataacgac tggtgtgggt
     1501 gctccggtgc ccatgcttgc gtccttggtg aaggggctgt gttccaatcc ccattcacct
     1561 ggacgcaagc cctatctttc aagagtaaaa cgggttacac cccgcatatc cctcggtggt
     1621 gttcggcgag aagtctcgaa atgtcgtctc gctggccaac ctctcggtga tcaccggcca
     1681 gatcggtcgc cccggcgccg gcgtctgccc gctgcgtggc cagaacaacg
//
LOCUS       DBNGZC010000462         1419 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1112124, whole genome shotgun sequence.
ACCESSION   DBNGZC010000462 DBNGZC010000000
VERSION     DBNGZC010000462.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1112124"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="AC16G0_08090"
     CDS             complement(<1..574)
                     /locus_tag="AC16G0_08090"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099614.1"
                     /translation="MHKRSIGMTRRRFLTSAAAVGAATVLAPSRRPALAQGAKPTISF
                     WNGLTGADGKVMDDLIGQFTKETGIAVEQQRIVWPDLYAKLQVSTPAGEGPDICLIHT
                     VEVPHFAGDGILDAIDNRTLEAKGFRGEDYLPSPWNGGSYQGKRYTIPLDVPQHIFFL
                     NPKLMKAAGFANPDGSLKLPGSKDELLQMGA"
     gene            906..>1419
                     /locus_tag="AC16G0_08095"
     CDS             906..>1419
                     /locus_tag="AC16G0_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HIR5099615.1"
                     /translation="MAKIGIDLDRKIGTVDRRIFGNFIEHIGRCIYGGVYEEGSPLSD
                     ERGFRRDVLEAARPLRVPILRWPGGNFVSGYHWLDGVGPKDSRPRKTDLAWYAEESNR
                     YGTDEFIEYCRVLDTEPCICVNMGSGTMDEAQAWLEYCNGTGNTHWANLRRKHGHAEP
                     HRVKYWGLDNE"
BASE COUNT          260 a          399 c          511 g          249 t
ORIGIN      
        1 tggcgcccat ctggagcaac tcgtccttgc tcccgggaag cttgaggctc ccatccgggt
       61 tggcgaagcc ggctgccttc atcagtttgg ggttcaggaa gaagatgtgc tgcgggacgt
      121 ccagcggaat cgtataccgc tttccctggt aggatccgcc gttccatgga gagggcagat
      181 agtcctcgcc gcggaaaccc ttggcctcca gcgtgcgatt gtcgatggca tcgaggatac
      241 cgtcgccggc aaagtgcggg acctcgaccg tgtggatcag gcagatgtct ggtccctcgc
      301 cggccggggt cgaaacctgg agctttgcat acagatcggg ccagacgatc ctctgctgct
      361 cgacagcaat cccggtttcc ttggtgaact gaccgatcag gtcgtccatc acctttccgt
      421 cggccccggt gaggccgttc cagaaggaga tggtcggctt ggctccttgg gccaaggcgg
      481 ggcgccggct cggggccagg acggtggcgg cgccaacggc tgcggcgctg gtgagaaagc
      541 gacggcgggt cattccgata gagcgcttgt gcatggaagt ccctccccct gcgaggatgt
      601 gtgtgaggaa gtctggttgc ggcgtggagc ggactatacc aaccccgttc aacccgtgtc
      661 aaggctgcga attggccggc cggcgcgtct ggagaatggg tacgtccgga tggcagggca
      721 gattgatcgg agggatcgcg gggggatgtc tcggcggcga agatggtcca tgggcgattg
      781 acaccccctg ctgggctggc ctatattcac gccgccgcgg ccgggatgaa caggagaggt
      841 ggagtccgga ccgcgacgtg ggtgcataga ctctgtccga aggctgcaac ccagggaggc
      901 gtgacatggc gaagatcggg atcgatctgg atcggaagat tggcaccgtc gatcggcgga
      961 tctttggcaa cttcatcgag cacatcggcc gatgcatcta cggcggcgtc tacgaagagg
     1021 gctcgccgct gagcgacgag agggggtttc ggcgggatgt cctggaggcc gcccgccccc
     1081 tgcgggtgcc gatcctccga tggccggggg ggaactttgt gagcgggtat cactggctgg
     1141 acggggtggg gcccaaggac agcaggcctc gaaagacgga cctggcatgg tatgccgagg
     1201 agtcgaaccg atacggcacg gacgagttca tcgagtactg ccgggtgctc gacaccgagc
     1261 cctgcatttg cgtgaacatg ggatcgggaa cgatggacga ggcccaggcc tggctggaat
     1321 actgtaacgg cacggggaac acccactggg caaacctccg ccggaagcac ggccacgccg
     1381 agccgcaccg ggtcaagtac tgggggctgg acaacgaga
//
LOCUS       DBNGZC010000463         2599 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_821561, whole genome shotgun sequence.
ACCESSION   DBNGZC010000463 DBNGZC010000000
VERSION     DBNGZC010000463.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_821561"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="AC16G0_08100"
     CDS             <1..676
                     /locus_tag="AC16G0_08100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5099616.1"
                     /translation="AERLEEAAALARAGGGTVLTFSGDVADKARVKQVVQETVEHFGR
                     VDILVNNAGTNTYHRNLADLSVEDWSLVMNTNLTGAFLFTRQVLPHMREAGKGHIINI
                     SSAAGMQPSAPAGVAYSASKHALHSLTGSINTEERRHGIRACVIAPGETDTPNLDLRP
                     RPPSKEDRATTLRPEDIANAVVYVASQPDRVAVELLLINPTVGRNYQADYERYVAEGH
                     TRIALD"
     gene            complement(883..1860)
                     /locus_tag="AC16G0_08105"
     CDS             complement(883..1860)
                     /locus_tag="AC16G0_08105"
                     /inference="COORDINATES: protein motif:HMM:NF028479.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HIR5099617.1"
                     /translation="MASVPRFQVVEGWEKLPPGYQHQDVDGVAVDSRDRVYLMTRRDA
                     RVIVYERDGSFVASWGEGIFTPRTHGIAVGPDDMVYSVDDGDHTVRKFTPDGKQVMMI
                     GTPGKSTDTGYDGKTIASIKRGGPPFNRPTDVAVAPDGEIYVCDGYGNARVHRFTAEG
                     KLLQSWGEPGNGPGEFHLPHGIGVSPDNRVFVTDRENDRLQIFSRDGQFLDMWTHLQR
                     PTDISFDKEGRVYVSELWWRVGQSSFTNGKIKWDLPGGVRVLDLQGNVLLHWCSADRE
                     APGNFVAPHTICVDSRGDLYVGEVTWTFGVSRGGVREDAHTFQKFARKA"
     gene            2129..>2599
                     /locus_tag="AC16G0_08110"
     CDS             2129..>2599
                     /locus_tag="AC16G0_08110"
                     /inference="COORDINATES: protein motif:HMM:NF016553.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099618.1"
                     /translation="MADTSQVSNPEAGLTSLESLAELSDLLSRTRSRLEMMRLLGVFL
                     RGLPREDRPLAARLLIGRPLPEGDPRKLDVSGVTLWRVASAVAGAPPASQAVWGEAVD
                     VGEAVERLFGSGARVPVKPSLSLQDVAGAFESMAAQRGRGARKAREEILAKLLER"
BASE COUNT          447 a          878 c          815 g          459 t
ORIGIN      
        1 cgccgaacga ctggaggagg ccgcggccct ggcgagggcc ggcggcggaa cggtgctgac
       61 cttttccgga gacgtggccg acaaggcgcg ggtgaagcag gtggtccagg agacggtcga
      121 gcacttcggc cgcgtcgaca tcctggtcaa caacgccggc accaacacgt atcatcgcaa
      181 cctggccgac ctctccgtcg aggactggtc cctggtgatg aacaccaacc tcacaggcgc
      241 gttcctgttc acgcgccagg tgctgcccca catgcgagag gcgggcaagg ggcacatcat
      301 caacatctca tccgctgccg gcatgcagcc gagcgccccc gcgggggttg cctattcggc
      361 ctcaaagcac gccctccact ctctcaccgg ttcgatcaac accgaggagc gtcgccacgg
      421 gattcgggcc tgcgtcatcg ccccaggcga gacggatacc ccgaacctgg atctgcgtcc
      481 gcgcccaccc tccaaggaag accgggccac gacgttgcgt ccggaggaca tcgccaacgc
      541 cgtggtctac gtcgccagcc agccggaccg cgtcgccgtt gagctactcc tgatcaatcc
      601 gaccgtgggg cgcaactacc aggccgatta cgagcggtat gtggccgagg gccatacgcg
      661 gatcgccctc gattgatcca gccacccagg cggcagcgtc ccctcgggtg atttgcggac
      721 tgatgacgca aaggcccgcg cggtgacaca ccacgcgggc ctttgattca ctggcagaag
      781 ggggaagggt tcggtcctat cgtcccttcg ccgattcgct ggacgataca gacggcggcc
      841 tcagttccgt actcccggct agcaggcggc gttcccgccc tgtcaggctt ttcgggcgaa
      901 cttctggaac gtgtgggcgt cttccctcac gccgccgcgg gagacgccga aggtccaggt
      961 gacctcaccg acgtagaggt ccccccgcga atccacacag atggtatggg gcgcgacgaa
     1021 gttccccggg gcctcccgat ccgcgctgca ccaatggagg agcacattcc cctggaggtc
     1081 cagcacgcgc acgccccccg gcagatccca cttgatcttc ccgttggtga acgaggactg
     1141 gccgacccgc caccacagct cggatacgta cacccgaccc tctttgtcga aggagatgtc
     1201 cgtgggccgc tggaggtgcg tccacatatc caggaattgg ccgtcccggg agaagatctg
     1261 gagacggtcg ttctcgcgat ccgtcacgaa gacgcggttg tcgggggaga ccccgatgcc
     1321 atgggggagg tgaaactctc ccgggccgtt accgggttcg ccccaggact ggagcagctt
     1381 tccctcggcc gtgaaccggt ggacccgagc attgccgtag ccgtcgcaca catatatctc
     1441 cccatctggg gcgacagcca cgtcggtggg ccggttgaag ggcgggccgc cacgcttgat
     1501 gctggcgatg gtcttgccgt catacccagt gtccgtcgat ttcccgggag tgccgatcat
     1561 catcacctgc ttgccgtcgg gcgtgaactt ccgcactgtg tggtcgccgt catccacgct
     1621 gtataccatg tcgtccggcc cgactgcgat cccgtgggtc cggggcgtga agatgccctc
     1681 gccccacgag gcgacgaagg agccgtctcg ctcgtacacg atgacgcggg catctcgccg
     1741 agtcatgagg tagacccggt cccgcgagtc gacagccacg ccgtccacat cctggtgctg
     1801 gtagcctggg ggaagttttt cccaaccctc gacgacttgg aatctgggta cactcgccat
     1861 catattctcc tttttccacg caatgatagg tcgccgacaa ccacgctggc ctcaaggacc
     1921 acgaccggcg gattataccc ctcccgccac accaggcaag caatccttcc ctttggtcaa
     1981 aaggagcaga cattctcttg cccttcagcc cccgcttcgc cgtctctgtc gtgagggccc
     2041 ttcaattcgc atttcgaaat tccgcaattc gaaattcacc cggcgcctcc cctgggccgc
     2101 gagggactta catgctagac tcctcgccat ggcagacact tcccaggtga gcaatccgga
     2161 agccggcctc acaagtctcg agagtctcgc cgagctttcg gacctgcttt cgaggacccg
     2221 gagccggctg gagatgatgc gcctcctggg cgttttcctc cgaggtttgc cccgcgagga
     2281 tcgccccctc gccgctcgcc tcttgatcgg tcggccgctg cccgagggag atcctcggaa
     2341 gctcgatgtc agcggggtca ccttgtggcg cgtggcaagc gcggtggcag gggcgcctcc
     2401 cgccagccag gccgtgtggg gcgaggcggt ggacgttgga gaggcggtcg agcgtctgtt
     2461 tgggagcggc gccagggtcc ctgtcaagcc gagcctcagt ctgcaggacg ttgcgggggc
     2521 ctttgagagc atggctgccc agcgaggtcg gggcgcgcgt aaggcacgag aggagatcct
     2581 cgccaagctc ctggagcgc
//
LOCUS       DBNGZC010000464         3158 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2680090, whole genome shotgun sequence.
ACCESSION   DBNGZC010000464 DBNGZC010000000
VERSION     DBNGZC010000464.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2680090"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="AC16G0_08115"
     CDS             complement(<1..226)
                     /locus_tag="AC16G0_08115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5099619.1"
                     /translation="MTDSRAEVVVIGGGARGCSCAYHLAKAGVRVTLVEKDGIASACS
                     GATFALINVSHKDPGHYAALSLESAKLFAGL"
     gene            complement(378..608)
                     /locus_tag="AC16G0_08120"
     CDS             complement(378..608)
                     /locus_tag="AC16G0_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099620.1"
                     /translation="MIGDALFFEGAALLVVGGLLDVGRSITIAHVRALPRIGEPPPSV
                     RKPGRAFVLVIAGVLMCLQGVLLVRLFPLSRG"
     gene            complement(801..1415)
                     /locus_tag="AC16G0_08125"
     CDS             complement(801..1415)
                     /locus_tag="AC16G0_08125"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIR5099621.1"
                     /translation="MVEQPTIPQGSTALIFNDMINGNLRTKNNPDHNKLIEESGLIQN
                     SVRLVAGLRQRKVPIIWIRVERRADYADVVFPVTDMFLAGGRKRPPATVRGSYEAANV
                     DELPVQPEDQVVLKPRFNPFIGTDLDLQLRARKITTILFGGYSTNVGVESGVRTARDL
                     GYDVVAVSDCCWNVHKDLHEWSVTKIMPIFARVLTSEQVLTLLA"
     gene            complement(1459..2559)
                     /locus_tag="AC16G0_08130"
     CDS             complement(1459..2559)
                     /locus_tag="AC16G0_08130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5099622.1"
                     /translation="MPEVKSADAVVIGGGVRGCSIALFLARAGVRVVLVERRTVGELA
                     STGNGAQVNVTAKEPDHYTAFSLRSARMYPEFIASLEADVVLQQEGLLFATIHPKEME
                     EFRRRVEIRNRIPGLHAELLDARAAREILPALGPEVLGGYIARADGRVDVLQLLPAMG
                     RAAQRAGAVILRGTEVTGIEVAGNRVQEVLTTQGAIATPVVVNAAGVHVPHIGRMVGV
                     TIPVEAEHGHMAITQPAPPLLPIPTQHVAQWPSGAFTIGTTNKMIGYDNAVRPGWLPP
                     FLSEALHLLPALKTVNILRVFAHLRPLPPDRLPIYDRAAGLDGFYIAVGHSGITLAPL
                     TGKLFADWIVTGKPDKDLAPYSLSRFAASLTQ"
     gene            complement(2624..>3158)
                     /locus_tag="AC16G0_08135"
     CDS             complement(2624..>3158)
                     /locus_tag="AC16G0_08135"
                     /inference="COORDINATES: protein motif:HMM:NF037640.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR5099623.1"
                     /translation="PVRGEDMTTSVPNVYAAGDCAGVAGSFVAIEEGRIAGLAVAQAL
                     GRLSRDEFTKRMEPSRERLAGLTRLRKALDEISVPRPGLYALATDATILCRCEEVTLG
                     EIKAALVGGVTDMNEVKRATRMGMGNCQGRMCGPALQEIIARKCGVSPAEIRPLNPRP
                     PIKPVPITALAGHTEMD"
BASE COUNT          537 a         1106 c          962 g          553 t
ORIGIN      
        1 cgagccccgc gaagagcttg gcgctctcca ggctcagcgc cgcatagtgc ccgggatcct
       61 tgtgggagac attgatgagg gcaaaggtcg cccctgaaca ggccgacgcg atgccgtcct
      121 tctccacgag cgtgacgcgt acgcccgctt tcgcgaggtg gtaggcacag gagcagcccc
      181 gggcgccccc gccgatgacg accacctcag cccgagaatc cgtcatctcc acctcctcct
      241 cggcggccag actcggcccc gcaatggacg gctccgcaag atgccaggcg tcggacagac
      301 ctcagcgccg tgatttgcga cgtcgtactt tccccgcatc ctcatggctt gcaggctcac
      361 cacgcgatcc gccagcctca accccgggag agcgggaata gacggaccag cagcactccc
      421 tgcaggcaca tcaacacgcc tgcaatgacc aggacgaacg cccgcccggg tttccgtacg
      481 ctgggaggtg gctcgccgat ccgcggcaag gctcggacat gggcgatggt gatggagcgg
      541 ccgacgtcga gtaggccacc caccaccaac agcgccgccc cttcgaaaaa cagggcatct
      601 cctatcaatc tccgccacgg aagaccgagc aacccaacgc acaggccggt cagtacccca
      661 aggccttctg ccatcgcgag ggcccacagc agatacgatt tccaagtcga aggcatagcc
      721 tctcttgggt tgtcctggca ttcacccttg tcctcggggg ttcgtcgact ccttccctgc
      781 agccgccgat ccgcgcgggc tcaggctaga agcgtcagga cctgctccga ggtcagcacg
      841 cgggcgaaga tgggcatgat cttggtcacc gaccactcgt ggaggtcttt gtggacgttc
      901 cagcagcagt cgctgaccgc gaccacatcg tagccgaggt ctcgggccgt gcgcacgccg
      961 ctctccacac ccacgtttgt ggaatagccg ccaaacagaa tggtcgtgat cttgcgggcc
     1021 cggagctgga ggtcgaggtc ggtcccgata aagggattga agcgaggctt gagcacgacc
     1081 tgatcttcgg gttgcacggg cagctcgtcc acgttggccg cctcgtagga gccccgtacc
     1141 gtggcgggag ggcgcttccg cccgccagcc aggaacatat cggtcacggg aaacaccacg
     1201 tcggcgtaat ccgcgcggcg ctcgacccgg atccagatga tcggtacctt tcgctgccgg
     1261 agccccgcca ccagccggac cgagttctgg atgaggccac tctcctcgat cagcttattg
     1321 tggtcgggat tattcttggt gcgtaggttg ccattgatca tgtcgttgaa gatgagtgcg
     1381 gtgctgcctt gcgggatcgt cggttgctcg accatggtca cctccgggtg gcgttccgcc
     1441 gtttccggcc cgctgcgttt actgcgtcaa gctagctgcg aagcggctca gtgagtacgg
     1501 cgccagatcc ttatccggct ttcccgtcac aatccaatcc gcaaagagct tccccgtcag
     1561 cggcgccagg gtgatgccgc tgtgtcccac cgcgatgtag aatccgtcca gccccgccgc
     1621 ccggtcatag atcggcaggc ggtccggcgg tagcggcctc agatgcgcga acacccggag
     1681 gatgttcaca gtcttcaggg ccggcaagag gtgcagcgcc tcgctcagga agggcggcag
     1741 ccaccctgga cggacggcgt tgtcgtatcc gatcatcttg tttgtggtgc cgatggtgaa
     1801 ggctccactc ggccattggg cgacatgctg cgttggaatg ggaagcagcg gaggagccgg
     1861 ctgcgtgatc gccatgtgcc catgctccgc ctcaactggg attgtcacgc cgaccatgcg
     1921 tccgatgtgc ggaacgtgca cccccgcggc attgacgacc acgggggtcg cgatggcgcc
     1981 ctgcgtggtc agcacctctt gcacccggtt ccctgcaacc tctatacccg tcacctccgt
     2041 ccctcggaga atcaccgccc ccgcccgctg cgccgcccga cccatggccg gaaggagctg
     2101 gagcacatcc accctgccgt cggcacgcgc gatatagcca cccagaacct cgggccccaa
     2161 agccggcagg atttctctgg ctgcccgagc gtccaacagc tcggcgtgca gtccggggat
     2221 acggttccga atctccacgc gccgccgaaa ctcctccatc tctttcgggt ggatcgtggc
     2281 gaacagcagc ccctcctgct ggaggaccac gtcggcctcc aggctggcaa tgaactcggg
     2341 atacatgcgc gcgctgcgga ggctgaaggc agtgtagtga tcgggctcct tggccgtgac
     2401 gttgacctgg gcgccattcc cggtcgaagc caactcccca acggttcgcc gctccaccaa
     2461 gaccacccgg acgcctgcgc gggccagaaa cagcgcgatg gaacagcccc tcacgccgcc
     2521 cccgatcacc acggcatcgg cactcttcac ctctggcatg gataccaatt ccccctttcc
     2581 tccctgcggg tcccgtcgca atggcagccc gcccctccgg gcactagtcc atttcggtgt
     2641 ggccggcaag ggcggtgatc ggcaccggtt tgatcggcgg ccgtggattg agcggccgaa
     2701 tttcggccgg cgagacgccg cattttcggg cgatgatctc ttgcagcgcg ggaccgcaca
     2761 tgcggccttg acagttgccc atgcccatgc gggtcgcacg cttgacctca ttcatgtcgg
     2821 tcacgcctcc gaccagggcc gccttgatct ccccgagagt tacttcctcg caccggcaca
     2881 ggatggtggc atctgtcgcc agcgcataca gccctggccg cggcacggat atctcgtcta
     2941 gcgccttccg gagccgcgtg aggcccgcca gccgctcccg actcggctcc atgcgctttg
     3001 tgaactcgtc tcgcgagagc cggccgagtg cctgcgcgac ggccagcccc gcgatccgcc
     3061 cctcctcgat ggccacaaag ctgcctgcca cgccggcgca gtcacccgcc gcgtacacgt
     3121 tcggcacgct ggtggtcatg tcctccccgc ggacgggg
//
LOCUS       DBNGZC010000465         2629 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_335111, whole genome shotgun sequence.
ACCESSION   DBNGZC010000465 DBNGZC010000000
VERSION     DBNGZC010000465.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_335111"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="AC16G0_08140"
     CDS             <1..294
                     /locus_tag="AC16G0_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099624.1"
                     /translation="LLVLPTWLLIVLRDGRQGVREINNLVPARARLDFWSIVRIVDRS
                     LRAFLQQQVTQAFLVGAGIAAVAWGLDRLNIVDVKYPPASWSFRNSFRMLAVS"
     gene            complement(513..782)
                     /locus_tag="AC16G0_08145"
                     /pseudo
     CDS             complement(513..782)
                     /locus_tag="AC16G0_08145"
                     /inference="COORDINATES: protein motif:HMM:NF037041.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltokinase N-terminal cap-like
                     domain-containing protein"
     gene            complement(837..2132)
                     /gene="glgA"
                     /locus_tag="AC16G0_08150"
     CDS             complement(837..2132)
                     /gene="glgA"
                     /locus_tag="AC16G0_08150"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - alpha-1,4-glucan
                     glucosyltransferase (UDP-glucose donor) activity [Evidence
                     IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009250 - glucan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HIR5099625.1"
                     /translation="MTQSELKVLFLTREYPPDIYGGAGVHVDYLSRELAKLIPIEVRT
                     FGTQDIRSGNLSVRGHRFAQGEAGDAPPQFLMALQAIRVSASFAARPSDADLVHCHTW
                     YAHFGGLLVKILYGIPLVVTAHSLEPLRPWKREQLGRGADLAAWIERTVIQAADAVVA
                     VSQEMKADILHHFGVAPDRVHIIGNGIDTDEYRPVSASQRLDRFGIKADRPYVLFVGR
                     ISRQKGILHLVNAIPHLDPAAQVVLCAASPDSADIAREVEEAVGRVSAVPDRVIWIRE
                     MVDRPTAIELYSHAAVFCCPSIYEPFGIINLEAMACEIPVVASAVGGIKEVVVNGETG
                     YLVPVEQHPDRPFEVVNPDRFSADLALHINRLLADAPLRKAMGAKGRRRAEVLFSWHA
                     VARQTLSLYESLVRSGTRTSVAPDPRVPEFPSIPKGDVP"
     gene            complement(2119..>2629)
                     /locus_tag="AC16G0_08155"
     CDS             complement(2119..>2629)
                     /locus_tag="AC16G0_08155"
                     /inference="COORDINATES: protein motif:HMM:NF027968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR5099626.1"
                     /translation="NGVRTPMQWSADRNAGFSLTDPGQLYSPVIADPMFGYQGVNVEA
                     QLRSPSSLLHWMRRLIAVRKTSRVFGRGSLQFLRPANTRVLVHLREHRGETILLAHNL
                     SGSAEPVELDLREFRGAIPTEMLGEARFPPIGDAPYFLSLGPYGYYWLRLQTAGPQRE
                     TYGIEDDAV"
BASE COUNT          452 a          820 c          899 g          458 t
ORIGIN      
        1 ctgctggtcc tgccgacgtg gctgctgatc gtgctgcgtg atggcaggca gggtgtgcgc
       61 gagatcaaca atttggttcc ggcacgagcc cgtcttgatt tctggtccat cgtgcgcatt
      121 gtcgatcgct cgctgcgcgc tttcttgcag cagcaagtca cgcaagcgtt tctcgtcggc
      181 gccggtatcg cagctgtggc gtgggggctg gatcgcttga acatcgtaga cgtgaaatac
      241 ccgccagcat cctggagctt tcggaacagc ttcaggatgc tggcggtgtc gtagaggacc
      301 gaggtgttgc tctgctcggc ttgcacccgg gtcactttct cgggggggcc gggcaggatt
      361 tcggccaagc gctctgtcgg ggtgaattgg aagcttcccc gacgaccggg caacgatgcc
      421 cgcagccgga tctgttccag cagggccgcg caggaggcag gatcgtccat ggcatcgcgg
      481 aatcccgcct cggtctcgtc ggggacgacc gggacgaggt aggtttcatc tcccccgtcc
      541 gtgaaggcga cgtcgaacag ggccagaatc gccatcgttc ccggcagcgg ggcaaaatcc
      601 cgcggggtca cacccaccag cctccgggac ttgctgccga accagcgctg acctggcagc
      661 cagaccacaa ggcccgcggc cgcgcgggtc gccagctctt cgggtgggat cggggttcgc
      721 cgggtccggg ggagtctccc acttgcggct ggacgcctgg acgatccgga cttcgtaccc
      781 atgctggggt tactcccgtc ttgccgaggg gctagccacg agcggcatct cccatgtcaa
      841 ggaacgtccc ccttcggtat cgatggaaat tccggaaccc gggggtcggg ggcgacggat
      901 gtccgtgttc cggatcggac caaagattca tacagggaga gtgtttgccg ggcgacggcg
      961 tgccaactga agaggacctc ggcccgtcgg cgccccttcg cgcccatggc cttccgcagg
     1021 ggggcgtcgg ccagcagccg gttgatatgc agggccaggt cggcggagaa ccgatcaggg
     1081 ttgacgacct cgaaggggcg gtccggatgc tgctccacgg ggacgaggta accggtctcg
     1141 ccgttgacca cgacctcctt gatgccgccc acggcgctgg ccactaccgg gatctcgcag
     1201 gccatggcct ccaggttgat gatcccgaag ggctcgtaga tggatggaca gcagaagacg
     1261 gcagcgtggg agtacagctc tatggccgtc ggccgatcca ccatctctcg aatccagatc
     1321 acccggtcag gtacggccga aacccggccg accgcctcct cgacctcacg ggcaatgtcg
     1381 gccgaatcag ggctggcggc gcagaggacc acctgagcgg ccgggtccag gtgtgggatg
     1441 gcattcacca gatgcaggat ccccttctgg cgggagatcc ggcccacgaa gaggacatac
     1501 ggccgatccg ccttgattcc gaagcggtcg agacgctggc tggcactgac cggacggtac
     1561 tcgtccgtat cgatgccgtt cccgatgatg tgtaccctgt ccggggctac tccgaagtgg
     1621 tgcaggatgt ccgccttcat ttcctgggac acggccacca cggcgtcggc ggcctggatg
     1681 accgttcgct cgatccaggc ggccagatcg gctccacgcc ccaactgctc ccgcttccag
     1741 ggtcgcaggg gctccagcga gtgggcggtg acgaccaggg ggatcccgta caggatcttc
     1801 accagcagtc ccccgaagtg cgcgtaccag gtatgacagt ggaccaggtc ggcgtcgctg
     1861 gggcgggcgg cgaagctggc ggagacgcgg attgcctgca acgccatgag aaactgcgga
     1921 ggggcgtccc ccgcctcacc ctgggcaaag cggtgacctc gtacgctgag attgccggag
     1981 cggatgtcct gcgtgccaaa ggtccggact tcgatcggga tcagcttggc cagctcccgg
     2041 gaaaggtaat ccacgtggac gccggcgccg ccgtaaatat ccgggggata ctcccgggtc
     2101 aggaacaaga ccttgagttc agactgcgtc atcttcgatc ccatatgtct cccgctgagg
     2161 gccggctgtt tgtagccgga gccagtagta gccgtagggt cccaggctga gaaaataggg
     2221 ggcgtcgccg atgggtggga atcgcgcctc gcccagcatc tcggtgggaa tggccccgcg
     2281 gaactcacgc aagtccagct ccaccggctc tgccgagccc gagagattgt gggccaagag
     2341 gatggtttct ccccggtgct cccgcaggtg gaccagcacc cgggtgttgg cgggacgcag
     2401 aaactgcaga ctccctcggc cgaagacccg cgacgtcttc cggaccgcga tgaggcgccg
     2461 catccagtga agcagggaag aggggctgcg gagttgcgct tccacgttga ccccctggta
     2521 gccgaacatg gggtcggcga tcaccgggct gtagagttgc ccagggtccg tgagggaaaa
     2581 gcccgcgttg cggtcggccg accactgcat gggcgtccgt acaccgttg
//
LOCUS       DBNGZC010000466         5818 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2094762, whole genome shotgun sequence.
ACCESSION   DBNGZC010000466 DBNGZC010000000
VERSION     DBNGZC010000466.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5818)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2094762"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            652..1782
                     /locus_tag="AC16G0_08160"
     CDS             652..1782
                     /locus_tag="AC16G0_08160"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010130959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR5099627.1"
                     /translation="MLIRHHPICPWRRARAVCLLIGLSVFLLMSQVYPASSQGRGSGG
                     LTPEEEIIIGVYRAVSPAVVHITSTVLSQDFFFRAVPEQGTGSGFMVDDRGYILTNNH
                     VVENADSLEVTLADKSKVPAKLIGRDPNNDLAVIRISVPKEKLAPVRLGDSSQLQVGQ
                     MAIAIGNPFGLDRTVTRGVVSAVGRSLKADTGRQIRNVIQTDAAINPGNSGGPLLNSR
                     GEVIGVNTAIFSPSGGSVGIGFAIPVNTAKSILPQLIARGRASHPWLGISGADITPAV
                     VRALNLTTTEGVIISQVAPGGPAARAGLRGSQRRTRVGNYMIGVGGDIIMALDGEKIA
                     SVDDLTAFLDDRKKAGDEVRVEVLRDGNPVTIVVRLGELPEG"
     gene            1791..1988
                     /locus_tag="AC16G0_08165"
     CDS             1791..1988
                     /locus_tag="AC16G0_08165"
                     /inference="COORDINATES: protein motif:HMM:NF014678.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HIR5099628.1"
                     /translation="MLFSLDIIWIREGRIVHIEKRTPPLTSTGPERIYTATADQVPEV
                     PAGFTSQQQIRVGDKVQAVLP"
     gene            2320..3561
                     /locus_tag="AC16G0_08170"
                     /pseudo
     CDS             2320..3561
                     /locus_tag="AC16G0_08170"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
     gene            3558..4928
                     /locus_tag="AC16G0_08175"
     CDS             3558..4928
                     /locus_tag="AC16G0_08175"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR5099629.1"
                     /translation="MRMRSGVRVEARRSTVFCLLVLLSASVLCLRSSGAWAAAIGQRE
                     VLPSGMVLLVSEKHAVPIVTATMLIQAGAVLDPSDKSGLANLTAELLTQGTSTRSASQ
                     IGEAIEFVGGSLSVNAGQDVTTVSLSVLTKDLDLGLDLLADILLHPTFAPAEIQRKVQ
                     EVVADIKHKHEDPGEVSAEAFATLAFGKHPYGRPAEGTETSVPTISREDIVRFHDLYY
                     RPNRTILAVVGDVSPQDLRQRLEGRLGSWQAAGPAIQPPAPPAPLPGPVVRTIQRDVT
                     QANITLGHLGVRRDNPDYYAILVMNYILGGGGFNSRLVSKIRDENGWAYDIGSFFAPD
                     KYAGAFTVSLQTKNEKAQEAIQAVLAEMRRIREQPVSDAELSDAKAYLTGSFPLRLDT
                     SSKIARLLANIEFFELGLDYTDRFPGLIHAVTVADVQRVAQRYLDPDRYVLAVVADLQ
                     KARIKE"
     gene            5008..5607
                     /locus_tag="AC16G0_08180"
     CDS             5008..5607
                     /locus_tag="AC16G0_08180"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR5099630.1"
                     /translation="MLSRERSNIGFCRRLIRGGLVCLPLISLSLGILLGLAWPVFAAA
                     DPFEALSLQEPKEHVAAPDFTLPDGVGKKLRLKDLRGSVVFLNFFATWCEPCRLEMPG
                     MEQLSQAYKGKGLVVLAVGMHESVKRVRAFMQELKLSFPAVVDADGSVGFMYSIGPIP
                     TTYLITRDGQIRWRAFGARDWDTPEAHAYFAKLLSGGPR"
BASE COUNT         1068 a         1789 c         1888 g         1073 t
ORIGIN      
        1 cccttcctga gtcaccgaat cagactgtct cgctctgcct cccggaaggg tcaatgcatt
       61 cggatctcgt tgagaccgca aggtacgcga aggtgcgggg gcttgccttt gctcgatatc
      121 tcagctatcc tctcgtatct atgcaataac ctgggttaca gccagcgcgc agaaggggct
      181 gtggcgctaa tcgggaacag ggtttccgga cgagctacac caccttaatc ccttttctgg
      241 tttctgcaat cagagcagtc ccctgcaggc cagaattaga gtcgaatacc ccccaggcga
      301 agcgcgcagc cggctgtggt ctgggagaag gaggaacggc ctgctcttgg gcgaggtgaa
      361 ggtctacagt ctcgcaatcg aacccaaccc ccggtgctga gctggttgcg gactgcgcct
      421 gaccggacgg ggaatccagc gatcgagaga cgccgtatct ttcacaccta ggatccagcc
      481 gggtgtctgt cgtccgccga tggttccctg cgcagctgcg gacaagggtc gccgctccca
      541 aggatctctc atccgaaccc tcgccccata cccggcccgc ccgggaggcc ggcggggaaa
      601 tggatcatga aactggcggc ctgaatcccc ctcttggcgg aggagtcctg aatgctaatc
      661 cgacatcatc caatctgccc ctggcgtcgc gcgcgtgcgg tctgcctcct catcgggctc
      721 tccgtcttcc tgctgatgag ccaggtgtac cccgccagca gccaagggcg tggtagtggc
      781 gggctcaccc ccgaggagga aatcatcatc ggcgtctacc gggccgtgag cccggcagtc
      841 gtgcacatca ccagcaccgt gttgagtcag gacttcttct tccgggccgt gccggagcaa
      901 ggaaccggtt ccggtttcat ggtggatgat cggggctaca tcctgacgaa caatcacgtc
      961 gtggagaatg ccgacagcct cgaggtcacc ctcgcggata agagcaaggt cccggcgaag
     1021 ctcatcggtc gcgacccgaa caacgatttg gccgtgattc ggatcagcgt ccccaaggaa
     1081 aaactcgcgc cggttcgact cggagattcg agccagcttc aggtcggtca gatggccatc
     1141 gccatcggga atcctttcgg cctggaccgg accgtcaccc ggggcgtggt gagtgcggtc
     1201 gggcgcagcc tgaaggccga taccggccgc cagatccgca acgtgatcca gaccgacgcg
     1261 gccatcaatc cgggaaactc gggagggccg ctcctgaact cccgcggtga ggtcatcggc
     1321 gtcaacacgg ccatcttctc gcccagtggc ggatcggtcg gtatcgggtt tgccatcccg
     1381 gtgaacacgg caaagagcat cctcccgcaa ctcatcgctc gcggccgtgc cagccatccg
     1441 tggctgggca tcagcggggc cgacatcacg cccgccgtgg ttcgcgctct gaacctaacc
     1501 acgacggagg gggtgatcat cagccaggtg gcgcccggcg gtccggctgc ccgggcgggc
     1561 ctgcggggat cgcagcggcg cactcgggtc ggcaactaca tgatcggtgt ggggggcgac
     1621 atcatcatgg ctctggacgg cgagaagatc gcctccgtgg acgacctcac cgcgttcctg
     1681 gacgaccgga agaaagcggg ggacgaggtc cgtgtggagg ttctccgcga cggcaatcca
     1741 gtgaccatcg tggttcgatt gggcgaactg cctgagggat aggcaaggag atgctcttct
     1801 ccctggatat catctggatc cgggaggggc ggatcgtaca catcgagaag cggaccccac
     1861 cattgacctc aaccggtccg gagcggattt acacggccac cgcggatcag gtgccggagg
     1921 ttcctgcggg cttcacttcc cagcagcaga tccgcgttgg tgacaaggtt caggccgtcc
     1981 ttccctgacc ctgagatacc ttgacgtgac tgccggtaac atcccaggcc ggcctcttgc
     2041 cttccgggac gcaaagcgta tcgagcccag ggtcaccggt ctcctggagt gtttcccatg
     2101 tcgtgtccga tgccgctggc agggacaggg gggacttctt tcaatgcggg gaagattatg
     2161 ctgaagaagt tgaggagctg gggtctccga cgccttccat tgtgggctgc agttttggtg
     2221 ctcgcattgt atctgcctac ccaccagtcg gcgtcggccg cgagcctagc ggaccgcgtc
     2281 tttgaggcga cactgacgaa tgggctcaag gtgctcttgg tggaagagcc caaggccccc
     2341 gtcgtgagca tccaggtttg gtataaggtc ggttcgcgga acgagccggt ggggaagagc
     2401 ggcatctctc acatgatcga gcacatgatg ttcaagggga cgcccaccgt cggccccaag
     2461 cagttcgacc gcatggtgca gcagaacggg gggcacgaca acgcctacac cacggcggac
     2521 tacacggcct attacgagga cttcgccgcg gaccgggtgt tgctgggcct cacgctggag
     2581 gcggaccgga tggcgcacct cctcttggac gagaaggagt tcctgccgga gcgccaggtg
     2641 gtcatggagg agcgtcgcct gcgaatcgag gaccagcccg cgaacgtcct gggcgaggtg
     2701 atgcgggcga cggccttcct ggcccacccg taccgctggc ccgtcatcgg ctggtccagc
     2761 gacatcgaaa gttacactcg ggaagatctg gttcggtatt tccacgccca ttacgcaccc
     2821 aacaacgcga tcctgatcgt ggcgggtgac gtcaagaggg acgccctgtt cccgaagatc
     2881 gaggagctct tcgggaagat tccgcggggt cccgagacgc ccagggtcgt gaccgtggag
     2941 ccgaggcagg cgggggaacg gcgagtgtac gtgaagaagg aggcggagtt gcctgtattc
     3001 tcggccgtgt aacatgtacc gaacctcacg catcccgaca gcttcgccct ggatgttctg
     3061 gcttatgtat tgggcggtgg ggagagctcc cgcctgcacc agagcgtcgt gtaccacaag
     3121 caactcgcct cctacgcttc tgccgattac tccggcatcc atgcggaccc gtacctcttc
     3181 gggctgagcg ccggaccgct ccccggaaag tccgtcgaag aggtggagca ggccgtgtac
     3241 gccgaggtgg agcgcatcca gcgcgagccg gtgtcagaca gagagttaca gaaggccaag
     3301 aaccagatcg cctccgaatt cattttcgcg caagactctg tgcacagact tgcccgcctg
     3361 ctgggggagt ttgaggcggt ggcgagctgg cgactcttgg ccgggtacat cgacggcatc
     3421 cagaaggtgg cggcgccgga cgtgacgcgg gtggcacggg agtatctcac caaggagaac
     3481 cgcacggcgg cgattctgat cccgacgaaa ggggctgcgg cagcgagcgg cagtgtgcag
     3541 ggccagaagg gacagccatg aggatgcggt ccggggtgcg cgtggaggcg cggcggagta
     3601 cagtcttttg tctgctggtc ctcctgtcgg catcagtcct gtgccttcgc tcgtcggggg
     3661 cttgggcggc ggcgataggc cagcgtgagg tgctccccag cgggatggtc ctgttggtct
     3721 cggagaaaca cgccgtgccg atcgtgacgg ccaccatgct gatccaggcc ggcgccgtct
     3781 tggatccctc tgacaagtca ggcttggcaa atctcacagc ggagcttctg acccagggca
     3841 cgtccacacg ctcggcgtcc cagatcggcg aagccatcga gttcgtgggc ggttcgctta
     3901 gcgtgaacgc gggacaggac gtgacgactg tgtctctgtc cgtgctcacc aaggacctgg
     3961 acctcggcct ggatctcctg gcggacatcc tcttgcatcc gacctttgcc cctgcggaaa
     4021 tccagcgaaa ggtgcaggag gtggtagccg acatcaagca caagcacgaa gaccctgggg
     4081 aggtgtcagc ggaagcattc gctacgctcg cattcgggaa gcacccctat gggcggcctg
     4141 cggagggaac cgagacgtcc gtcccgacca tcagccggga ggacatcgtc cggtttcacg
     4201 acctgtatta ccggcccaac cgcacgatcc tggccgtggt gggggacgtg agcccccagg
     4261 acctcaggca gcgccttgag gggcgcctgg ggtcatggca ggccgcagga ccggcaattc
     4321 agccgcccgc gccgcctgcg ccactaccgg gaccggttgt gcgcacaatc caacgcgatg
     4381 tgacccaagc gaacatcacc cttggccatc tcggcgtgcg gcgggataat ccggactact
     4441 acgccatcct ggtgatgaac tacatcctgg gtggaggggg attcaactcg cgtctggttt
     4501 ccaagatccg ggatgaaaat gggtgggcct acgacatcgg gagctttttc gctcccgaca
     4561 agtatgccgg ggccttcacc gtcagcctcc agacaaagaa cgagaaggcc caggaggcca
     4621 tccaggccgt tctggcggag atgcggcgaa tccgagagca gccggtgagc gacgccgagc
     4681 tcagcgacgc caaagcctac ctgaccggca gcttccccct gcgcctggac acgagcagca
     4741 agatcgcccg cctgctggcc aacatcgagt ttttcgagct gggattggac tacaccgatc
     4801 ggttcccggg actcatccat gccgtgacgg tggcagacgt gcagcgagtg gcgcagcggt
     4861 atctcgaccc cgaccggtac gtcttggccg tggtggccga tctccagaag gcaagaatca
     4921 aggagtgatc tccgttatcc gagaagtcgt tccgatgcaa gggaagatac acgtgaggaa
     4981 atgctcgttg ttctttgagg aatcccagtg ctgagccgag agcgatcaaa tatcgggttc
     5041 tgcaggcggc tcattcgtgg cggtctggtc tgtcttccac tcatctcgct ctccctgggt
     5101 atcctgcttg ggttggcttg gcccgttttc gccgcagcgg atcctttcga ggcgctgagt
     5161 ctgcaagagc cgaaggaaca cgttgcagcg ccggatttca ccctgccgga tggggttggg
     5221 aaaaagctac gactcaagga ccttcggggg agcgttgttt tcctcaactt ctttgccacg
     5281 tggtgcgagc catgccggct ggagatgccg ggaatggagc agttgtccca ggcctataag
     5341 ggcaaggggc tggtggtcct ggctgtgggc atgcacgaga gtgtcaagag ggtccgcgcc
     5401 ttcatgcaag aactgaagct ctcgttcccg gcggttgtgg atgcggacgg ctctgttggt
     5461 ttcatgtaca gcatcgggcc cattccaacc acgtacctga taacccgtga cggccagatt
     5521 cgctggagag ccttcggggc ccgggactgg gatacccccg aggcccacgc ctacttcgcc
     5581 aagctgcttt ccgggggacc tcggtgagca atcggtcccg cgggcgaacg gcgcgaaggg
     5641 ccgcggggcg acatacctgg cccgaccctc ccgggccgca ggagtaaccc ggcatgccac
     5701 acacccaact gagtctcctc ttggccctgg ggggcggcct cctgtccttc ctctccccct
     5761 gcgtcctccc cctgttcccc tcctacctct ccttcgtggc cggggtctcc ctggagga
//
LOCUS       DBNGZC010000467         3239 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2191420, whole genome shotgun sequence.
ACCESSION   DBNGZC010000467 DBNGZC010000000
VERSION     DBNGZC010000467.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2191420"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="AC16G0_08185"
     CDS             <1..218
                     /locus_tag="AC16G0_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR5099631.1"
                     /translation="HIVENARGYIQLLRAHIDKENNILFPMADNVLSPEDQTELGEAF
                     ERFENEETGAGVHEAALKLLEELRAGA"
     gene            complement(310..2169)
                     /locus_tag="AC16G0_08190"
     CDS             complement(310..2169)
                     /locus_tag="AC16G0_08190"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF015859.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5099632.1"
                     /translation="MPRMRQACAWLVRRLLQRRRTVLTAAFLIGALLSAGAFFEESQD
                     ARLGVEQAFRGSASQQFRAIKEEIEEAVDRGTSVAELFQAFGSVDRRQFREVVVPQLG
                     RAPGILAIFSAPRVPDGDRSRYEAAGRQKWGPGVHLFDRGKQDRAAGPRPEYYPVDYH
                     ETLRGPVNERVVGQDLGSELAGLEALLRARDTGQPAMTASLRLFNGEKGVHVYWPIYE
                     RGAPRSSVDERRENLLGFVGAMFDIEALVDSALGEMPPQGIDITLSDDGAPVGERVLI
                     DRPSRLKDPPRPWLGQRLIEGHIRSGLPQTNTLEVGGRRWSVAFAPTEGYVSARTGTD
                     MWDHLLLGLLTTGLVGGYLLMLMRRTDVVERQVAARTQELQVALGEKTRAEAVARRLT
                     QQVLSVQEEERRHLSRELHDEAGQNLTTLVLNLRLLQADVPEGQASLRQRLREAGEMI
                     QATVEKIRLLARDLRPPGLDTLGLNASLKGLCDSVARQTAVSITYAGAEVSPLPDGVT
                     ICLYRVLQEALTNVVKHAHAERVRVRLRNGAESVFLSVEDDGQGFDPGAVKCPGNGNG
                     KGNGSRSLGLVGMQERLELVGGRLEIVSRRGHGTRLMARVPARGVEPADPVRH"
     gene            complement(2223..2855)
                     /locus_tag="AC16G0_08195"
     CDS             complement(2223..2855)
                     /locus_tag="AC16G0_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5099633.1"
                     /translation="MSLRILLADDHQFVRLGLKALLEREGYEVVGEASDGLEAVRLAQ
                     CLQPDVAVLDICMPLLNGIAAASQILQHSPKTKPILLTMYTEDVYVTEAFRAGMRGYV
                     VKSQMADDLLVAIRKVAEGRLYLSPAISDAVIQSFVDKSDLPTVTLSARETQVLQLLA
                     EGKTTKESGELLGISVRTVEAHRRNTIAKLGIRDRAGLVRYAIRRGVIQP"
BASE COUNT          559 a         1117 c          986 g          577 t
ORIGIN      
        1 ggcatatcgt cgagaacgca cgaggctata tccagctctt gcgcgcgcac atcgacaagg
       61 agaacaacat cctgttcccc atggccgaca acgtcctgag ccccgaggat cagacggaac
      121 tgggcgaggc cttcgagcgg ttcgaaaacg aggagacggg cgccggggtc cacgaggcgg
      181 cgttgaagct cctggaggag ctccgggccg gggcgtgatg ccagaccgaa tcttgtgaat
      241 cgaaggattg aatcccggat gagtcgtggt cggggacggc cagcaggccg tccccgaaat
      301 tgtctcgcat cagtggcgga cgggatcggc aggctctacc cctctcgcgg gaactcgtgc
      361 catcaggcgt gtcccatgac ctcggcgaga cacgatctcc aggcgacccc ccaccaactc
      421 cagccgttcc tgcatcccga ccaaccccag gctccgcgag ccatttcctt ttccattccc
      481 gttcccaggg cacttcacgg ccccagggtc gaagccctgt ccgtcatcct cgacggacag
      541 gaacacgctc tcggcgccat ttcgcagcct cactcgaacc cgctcggcat gggcgtgctt
      601 gaccacattc gtcagggcct cttgcaggac ccggtagagg caaatggtga ccccatccgg
      661 caacggcgag acctcggcgc ctgcgtacgt gatcgacaca gccgtctggc gggcaacgct
      721 gtcgcacagg cctttcaggc tcgcgttaag acccagggtg tcgagaccgg ggggccgtag
      781 gtcccgggcc agcaggcgga tcttctccac ggtcgcctgg atcatctcac ccgcctcccg
      841 gaggcgttgc cgcagtgatg cctgtccctc gggtacatcc gcctggagca agcgcaggtt
      901 gagcacgagc gtggtgagat tctggcccgc ctcatcgtgc agctcgcggg acaggtgccg
      961 ccgctcctcc tcctggacgg agaggacctg ctgggtgagc cggcgtgcca cggcctcggc
     1021 cctggttttc tccccgagcg ccacttgcag ttcctgggtg cgggccgcca cctgccgctc
     1081 cactacgtcc gtccgccgca tcagcatgag gaggtagccc ccgacgagac ccgtggtcag
     1141 gagcccgagg aggaggtggt cccacatgtc cgtgccggtc cgcgcgctca cgtagccctc
     1201 ggtcggggcg aaggccaccg accagcggcg cccgccaacc tcgagcgtat tggtctgagg
     1261 caggccggat cggatgtggc cctctatcag cctctgtccc agccagggcc gcgggggatc
     1321 cttgaggcgg gatgggcggt caatgagcac ccgctcgccg acgggtgccc cgtcgtccga
     1381 aagggtgatg tcgatcccct gaggaggcat ttccccgagc gccgagtcca ccaacgcctc
     1441 gatatcgaac atcgcgccca cgaatcccag gaggttctcc cgccgttcat ccacgctgct
     1501 ccggggcgcc cctcgctcgt aaatcggcca gtagacatgg actcccttct cgccgttgaa
     1561 cagccgaagg ctcgcggtca tggcgggctg gcccgtatcg cgcgcccgca ggagcgcctc
     1621 caagcctgca agctcggagc ccaagtcctg acccacgacc cgctcgttga ccggccccct
     1681 gagggtctca tggtaatcga cagggtagta ctcgggccgc ggacccgctg cccggtcctg
     1741 cttgcccctg tcgaagaggt gaacgcccgg cccccacttc tgcctaccgg ctgcctcgta
     1801 tcgtgaccga tccccatccg gaacccgggg ggccgagaag attgccagga tccccggtgc
     1861 gcggccgagc tgtgggacca cgacctcccg gaactggcgt cggtcaacgg acccaaaggc
     1921 ctggaagagt tccgccaccg aggtgccgcg gtcaacagct tcctcgatct cttctttgat
     1981 tgcccgaaac tgttgcgacg cgctcccgcg gaacgcttgc tctaccccaa gtcgtgcgtc
     2041 ttgcgattcc tcgaaaaacg cgcctgcgct caggagcgcc ccgataagga atgcggcggt
     2101 caggaccgtt cggcgacgct ggagaagccg tcgaacgagc caggcacagg cctgtcgcat
     2161 gcgtggcatg agtgtgagat cctcggcgga tggcatcccc tgtccctgtc gtcagcagag
     2221 tgtcacggct ggatcacacc gcgccggatg gcgtaacgga cgaggcctgc cctgtcacgg
     2281 atgcccagct tcgcaatggt attgcgacga tgggcctcca cggtcctgac gctgatcccg
     2341 agcaattccc ctgactcctt ggtcgtcttt ccctcggcca ggagttgcag gacctgcgtc
     2401 tctctagcag agagggtaac cgtgggcagg tcggacttgt ccacgaagct ctggatgacg
     2461 gcatccgaga tcgccgggct caggtacagc ctgccctcgg ccaccttccg gatcgccacc
     2521 agcaggtcat cggccatctg actcttcacc acgtagcccc gcatccctgc gcgaaaggcc
     2581 tcggtcacgt agacgtcctc cgtatacatg gtgaggagga tgggcttcgt cttgggagag
     2641 tgctggagga tctgactcgc cgccgcgatg ccgttcagca gtggcatgca gatatccagg
     2701 acagccacgt caggttggag acactgagcc agacgaacgg cctccaaccc gtccgaggcc
     2761 tcgcccacga cttcgtaccc ctcccgctcc aggagggcct tcaagcccag ccgaacgaac
     2821 tggtggtcgt cggccaagag aatccgcagg gacacatcac acctcccgga tgactatcgg
     2881 tttcacgctg gacctgttca tgccttcgcg acagctatcc tgaggcccgg cggcggggcc
     2941 catcttcgcg caagccagtt gcgctgcgcc gcgcggactg gagctgcctg gaggtccatc
     3001 cataatgctc agaatgatat cgtctgctgg tggcaagtaa atcggacttc ctccctattt
     3061 cctgtaaggc tttgtcctgc atgtttgtcg gtgttcatcc tacatgcggc gtcctcacct
     3121 ccttggccgc ctggaagccg acgagcacgc cgtcctctcc cgccgtcccg ccttcggtgc
     3181 accgctccac cgcgagaaac ctcatctgga gcaaaggcga gtctacttcc cgattgcat
//
LOCUS       DBNGZC010000468         6706 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1213747, whole genome shotgun sequence.
ACCESSION   DBNGZC010000468 DBNGZC010000000
VERSION     DBNGZC010000468.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6706)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1213747"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /gene="ybgF"
                     /locus_tag="AC16G0_08200"
     CDS             <1..317
                     /gene="ybgF"
                     /locus_tag="AC16G0_08200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HIR5099634.1"
                     /translation="IAGFRSYIQTYPKSSLVANAQYWLGESYYSQKNFAQATQEFDVV
                     VRDFPDSPKVPSALFKQGDAYLQLGDTKRATTVLCELISNRKFAKTREAQLARDKNVR
                     CR"
     gene            complement(413..1768)
                     /gene="dnaA"
                     /locus_tag="AC16G0_08205"
     CDS             complement(413..1768)
                     /gene="dnaA"
                     /locus_tag="AC16G0_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR5099635.1"
                     /translation="MNGAVTSSNAKELWDEALSVIRSRISRQSFEAWFRPLNLGTVEE
                     HRINVMLPNRFFKEWFEEHYLGLLRSALEDLMFSKVQVNLLLPEKEAASPKPAREEPT
                     ERRAPRRPRDGAAQLNPKYTFDTFVVGSSNQFANAASLAVAEQLSKAYNPLFIYGGVG
                     LGKTHLLHAVGHLAMQRDPRIKVSYVSSEKFTNDLINSIRFDATVEFRNRYRSLDLLL
                     IDDIQFIAGKERTQEEFFHTFNDLYDSSKQIVISSDSLPREIPTLEERLRSRFEWGLI
                     ADIQPPDLETKAAILRKKAQAQGVRLPDEVSLFIAKHVKSNIRELEGSLVRLAAQASF
                     TNREVSLELAQEVLKELTAEQQRIPTIPGIQKVVADFYGVRVDDLRSRGRNKSIVLPR
                     QVAMYLCREVVKASLPDIGDGFGKDHSTVIHACEKVKRKIAGEEVFRKQMDELSVRLS
                     A"
     gene            2483..3703
                     /locus_tag="AC16G0_08210"
     CDS             2483..3703
                     /locus_tag="AC16G0_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099636.1"
                     /translation="MRHTARWVGLGLAAIMLMGGRSEAGAQSPIKIGEINSYSGIAAG
                     FTLPYRQAVEMAVDEVNGAGGLLGRKVEVLFRDDKGNPAEAIKHAQGLVTEEKVSLLA
                     GTFLSNVGLAVSDFARQNRIPFVAAEPLTEAITWSQGHRYVFRVRPNTYSQGRVLAER
                     AAKLPYRKWAAIGPNYEYGHRAWETFWARLKELKPDVQVVAEHWPTLGQIEPSGYVTA
                     LQSEKPEAVYVSLFGSDWIAFVREASQRGLFQRAFYVGILLGEPEYMDPLKLEAPEGM
                     LVTGYPWYDIQTPSHRDFVARFEKRTGRTPVMGSLVGYITFLSIFEAIRKAQSTEAEK
                     MAAALEGLRVETPIGPITFRPFDHQSTMGCWVGMTKRDPKRGVGIMQNWEYVPGDKLL
                     PSEAEVRKMREVAR"
     gene            3807..5642
                     /locus_tag="AC16G0_08215"
     CDS             3807..5642
                     /locus_tag="AC16G0_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099637.1"
                     /translation="MQLLTGLTNATFLFLIASGLSLIFGVTRVINFAHGAFYMLAAYL
                     TYTLAASLPLGGASYYVAVLLASLGVAVLGGVLEVCLLRRVYGAPELYQLLLTFALVL
                     VIGDAVKFSWGTENKLGPVPPGLAGSVSIFDQAVSRYDLSMLLLGPAVALGLWWVFRH
                     SRWGILVRAATADREMAGALGVNQAWLFTSVFVVGTWLAGLAGALQVPRQALTSVMHA
                     SVLAEAFVVVVIGGMGSIFGALLGAVLIGVLQALGILLLPPGFDLAIMFVLMAAVLIL
                     RPWGLLGRPEGSARPQGEAAITADGGGAVSTRALCLTLLGMIILPLILPTFYVWLLVE
                     VLAFSLFAGSLQLLMGTGGMVSFGHAAYFGLGAYGAALAMKLGGLAMPVAFWMGPLLA
                     GLAALIFGAFCVRLTSIYFAMLTLAFAQISFAVIHQWYDVTGGDNGILGVWPAPYLAA
                     PVRFYYLALLVGAGGLALLWRITGSPFGCTLRAARDHALRCGAIGVNVRAHQLQAFVV
                     AGILAGVAGSTFAFLKGSVFPEYLGIPMSVESLIMVLLGGISTLVGAPVGAAAYKILD
                     TLVTNATDYWQAALGGILILVVVGFPQGLVGFWLERRSRTGGDRG"
     gene            5635..6402
                     /locus_tag="AC16G0_08220"
     CDS             5635..6402
                     /locus_tag="AC16G0_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014246860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099638.1"
                     /translation="MGETPLLEVRSVSKAFGGVQAVRDVSLRLSPREVRAVIGPNGAG
                     KTTLFNLLTGHLACDQGAVLFKGERISGLPPHRIWRRGISRTFQITATFRSLSVLQNV
                     QVAILSHRGRSREMFSITRRLVREPAAELLEQVGLQGLAERACGALSYADLKRLELAL
                     ALANDPEVLLLDEPTAGMAPQERMDLMALVLKTVAERGLGVLFTEHDMDVVFAAAGRI
                     LVLHQGRMISEGTPQEVRASRAVQEIYLGGPAQTDGW"
     gene            6403..>6706
                     /locus_tag="AC16G0_08225"
     CDS             6403..>6706
                     /locus_tag="AC16G0_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5099639.1"
                     /translation="MTADEKKMLEVADLEAYYGPSHILSDICLHTGRGEVVALLGRNG
                     AGKSTTLKTIMGLVPPAAGRVRFQGEDLAGQPPYAICRRGIGFVPQERRIFPDLTVG"
BASE COUNT         1100 a         2067 c         2210 g         1329 t
ORIGIN      
        1 ccatcgccgg ctttcgcagc tatatccaga cctatccgaa gtccagtctg gtggccaacg
       61 cgcaatactg gctgggggaa tcctattaca gccagaagaa cttcgcccag gccacccagg
      121 aattcgacgt ggtggtccgg gacttcccgg atagccccaa ggtccccagc gcgctgttca
      181 agcaggggga tgcctacctg cagttggggg acacgaagcg ggcgaccacg gtcctctgcg
      241 agttgatctc caaccggaag ttcgccaaga cccgggaggc acagctcgcg cgggacaaga
      301 acgtccgctg ccgctagcac ccttcggact gccgaaacaa atggcccggg acttctgtcc
      361 cgggccgttt ctgtcgattc ctcggccgtc tcgtctcgcc ttgtcctgcc cctcaggcgg
      421 atagccggac gctcagctcg tccatctgct tccggaagac ctcctcgccg gcgatcttcc
      481 gcttcacctt ctcgcaggcg tggatgacgg tggaatggtc cttgccgaag ccatcgccga
      541 tatcggggag ggaggccttc acgacttcgc ggcagagata catcgccacc tggcgcggca
      601 ggacgatgga cttgtttcgt ccccgcgagc ggaggtcgtc cacccgcacc ccgtagaagt
      661 cggccacgac cttctggatg ccggggatgg tggggatccg ctgctgctcg gctgtgagct
      721 cctttaggac ctcctgggcc agctccaggc tcacttctcg gttggtgaac gaggcctgag
      781 cggccaggcg gaccagagag ccttccagct cccggatgtt ggacttcacg tgcttggcga
      841 tgaaaaggga gacctcgtcc gggaggcgca cgccctgcgc ctgcgccttc ttgcgcagga
      901 tggcggcttt ggtctccagg tccggcggct ggatgtcggc gatcaggccc cactcgaacc
      961 gcgaccgcag gcgttcctcc agcgtcggaa tctcccgggg cagactgtcc gagctgatca
     1021 cgatttgctt ggaggagtcg tacagatcgt tgaaggtgtg gaagaattct tcctgggttc
     1081 gctccttgcc cgcgatgaac tggatgtcgt cgatgagcaa gaggtccagg ctgcgatacc
     1141 ggtttcgaaa ttccaccgtg gcgtcgaagc ggatcgagtt gatgagatcg ttggtgaact
     1201 tctccgagga aacataggag accttgatcc ggggatcccg ctgcatggcg agatgcccaa
     1261 cggcgtggag cagatgcgtc ttccccagac ccacgccgcc gtatatgaat aatgggttgt
     1321 acgccttgga gagttgctcg gcgacggcca aggaggcggc gttggcgaac tggttgctgg
     1381 agccgaccac gaatgtgtcg aaggtgtact tcgggttgag ctgagccgct ccgtcccgtg
     1441 gccggcgggg ggcgcggcgc tcggtcggct cctcccgggc gggcttgggc gatgcggcct
     1501 ccttttcagg gagcaggagg ttcacctgga ccttggaaaa catcagatcc tcgagcgccg
     1561 agcggaggag ccccaggtag tgctcctcga accattcctt gaagaagcga ttggggagca
     1621 tgacattgat gcgatgttcc tcgacggtcc ccaggttcag aggacggaac caggcttcga
     1681 agctctgccg gctgatgcgg ctgcggatca ccgagagggc ctcatcccag agctcctttg
     1741 cattcgaaga cgtgaccgct ccattcatgc tcagccctcc aagtgattta tccacagccc
     1801 atccaactgg ctaacctatt gatatgttga gaccgatcta actcccggtg cgctcaagcc
     1861 gcttccagag ccagccgaaa gttatccaca gctttttcca caggtgtgga taactggaac
     1921 ctcttgtttt tctcggagtc tcgggggatt atccccagaa cagccggacg tgcgggactt
     1981 gtgcatgtat ggaacatatt gaaaacaaac actttctggt tattcagggc acgaaggaga
     2041 ccccttgtag cgcagaccgg ataccggtgt caagtccctt ttttgaattt tttttcatgg
     2101 tccccgggca gtttcgaccc tgggcctcca gcgaagcaag gacagtccac gaaccaatac
     2161 cagcgcgcct cagagctatt gtacgggggt ctccccggcc aaccgggggg cccctgatcc
     2221 ctttgggcgg agccatttcc cttgacacgc tgttatgggc tgaatagatt ggaccaggtt
     2281 tcccggatac agagtgtgcg ccgcccttgg gatgtcgcct gctgacaagc tcctcggtgg
     2341 ctcagctcag atcgcgaacc cggatttttt cattgcccta ttacccgatc ggaagcgagg
     2401 actcaacttg aatgatcgat tggtttgggc aggcattcaa ggcccaaagc gaggacccga
     2461 tcgagctatc ggagcaggga gcatgagaca cacagcacgc tgggtcggtc tgggattggc
     2521 ggccatcatg ctaatgggcg ggaggtcgga agcgggcgcc cagtccccca tcaagatcgg
     2581 agaaatcaat tcctacagtg ggatcgctgc cggcttcacc ttgccctacc ggcaggcggt
     2641 tgagatggcg gtggatgagg tcaatggcgc agggggactt ctcggacgaa aggtcgaggt
     2701 cctgttccgg gatgacaagg ggaacccggc cgaggcgatc aagcacgccc agggtctcgt
     2761 gaccgaggag aaagtgagcc tgctggcggg gaccttcctg agcaatgtcg ggcttgcggt
     2821 ctctgacttt gcccgtcaga accggatccc tttcgttgcg gctgagccgt tgaccgaggc
     2881 catcacctgg tcccagggac accgttacgt tttccgcgtg cgccccaata cctattcgca
     2941 agggcgggtc ctggcggagc gggcggccaa gcttccctac cggaagtggg cggccatcgg
     3001 tccgaactac gagtacggcc accgcgcctg ggaaaccttc tgggcccggc tgaaggaatt
     3061 gaaaccggac gtccaagtgg tcgccgagca ctggccgacg ctgggccaga tcgagcccag
     3121 cggctatgtc accgcgcttc agagcgagaa gccggaggcc gtctatgtct ccctgttcgg
     3181 cagcgactgg attgccttcg tgcgggaggc gagccagcgc ggcctcttcc agcgggcctt
     3241 ctacgtcggc attctcctcg gcgaaccgga gtacatggac ccgttgaagc tggaggcccc
     3301 cgaggggatg ctcgtcacgg gctacccctg gtacgacatc cagacaccgt cccaccggga
     3361 cttcgtggcc aggttcgaga agcggacggg gcgcaccccc gtcatgggga gcctggtggg
     3421 ctacatcacc ttcctttcga tcttcgaggc catccgcaag gcccagagca ccgaggctga
     3481 gaagatggcg gcggccctgg agggtctccg cgtcgagacg cccatcgggc cgatcacctt
     3541 ccgccccttt gaccaccagt ccaccatggg ctgctgggtg gggatgacca agcgcgatcc
     3601 caaacgcggc gtggggatca tgcagaactg ggagtacgtc ccgggagaca agctccttcc
     3661 atctgaggct gaggtccgaa aaatgcggga ggtggcccgc tgaccctccg gggcaggcag
     3721 gcggtccggg cctcaggggt ggtcgggacc cagcgggctt cggcctagat gccaaccgaa
     3781 cttccttcct tatgtcattc ttcctggtgc aacttctgac gggtttgacc aacgcgacgt
     3841 ttctcttctt gatcgcgtcg ggcctctcgc tgatctttgg agtgacccgg gtcatcaact
     3901 ttgcccacgg cgccttctac atgctggcgg cctatctcac ctacacgctg gcggcgagcc
     3961 tccctctggg tggggcgagc tattatgtgg ccgtcctcct ggcgtctctg ggagtggcgg
     4021 tgctgggagg tgtcctggag gtgtgcctgc tgcggcgcgt ctatggggcg ccggagctgt
     4081 accaattgct tctcaccttt gccctagtcc tggtgatcgg ggatgcggtg aaattctcct
     4141 gggggacgga gaacaagctg gggcccgtcc cccccggcct cgccgggtcc gtatcgattt
     4201 tcgaccaagc cgtctcccgg tacgatctct ccatgctcct gctcgggccg gcggtggcgc
     4261 tgggtctgtg gtgggtgttc cgccacagcc gatggggcat tctcgtccgt gccgccacgg
     4321 ccgaccggga gatggccgga gccctgggcg tgaaccaggc ctggctcttc accagcgtgt
     4381 tcgtcgtcgg gacgtggctt gcgggtctgg caggcgccct ccaggtaccg cgccaagccc
     4441 tgaccagcgt gatgcacgcc agtgtcttgg ccgaggcctt tgtggtggtg gtgatcggcg
     4501 ggatgggcag catcttcggg gcgctcctgg gtgcggtgct gatcggggtc cttcaggccc
     4561 tcggcattct cctgctgccc ccgggattcg acctcgccat catgttcgtc ctcatggcgg
     4621 cggtgctcat cctccgcccc tggggcctcc tggggaggcc cgaggggtcc gcccgcccgc
     4681 aaggtgaggc ggcgatcact gcggacgggg gcggcgcggt ctcgacccgg gcgctctgtc
     4741 tcacgctcct cggcatgatc atccttcccc tgatcctgcc cacgttctac gtctggctgc
     4801 tggtggaagt tctggccttc agcctgttcg ccgggagcct gcaactcctc atgggaactg
     4861 gcggcatggt gtcctttgga cacgccgcct acttcggcct gggggcctac ggagccgccc
     4921 tggcgatgaa gctcggcggg ctggcgatgc ccgtcgcctt ctggatgggt ccgctgctgg
     4981 cggggctggc ggccctgatc ttcggggcct tctgcgtgcg actcacgagc atctactttg
     5041 ccatgctgac tctggccttc gcgcagatca gcttcgccgt gatccaccag tggtacgacg
     5101 tcacgggcgg cgacaacggc atcctgggtg tttggccggc tccctacctg gcggcaccgg
     5161 tgcggttcta ttacctggcc ctcctggtcg gtgccggtgg actcgccctg ctctggcgca
     5221 tcacagggtc cccgttcggc tgcaccttgc gggcggctcg tgaccacgcc ctgcggtgcg
     5281 gggccatcgg cgtgaacgtg cgagcccatc aactccaggc cttcgtggtg gccgggatcc
     5341 ttgccggcgt ggcggggagc accttcgcat tcctgaaggg gagcgtattt cccgagtacc
     5401 ttgggatccc catgtcggtc gaatccctga tcatggtcct cctggggggg atcagcacgc
     5461 tggtcggcgc gccggtgggt gcggcggcgt acaagatttt agataccctc gtcaccaacg
     5521 ccacagacta ctggcaggcc gccttggggg ggattctgat tctggtggtg gtgggcttcc
     5581 cccaagggct cgtcgggttc tggctggagc gccgatccag gacgggaggc gaccgtgggt
     5641 gagacaccgc tcttggaggt gcgaagcgtc tcgaaggcct tcgggggggt tcaggcggtc
     5701 cgcgatgtga gcctccggct gtcccccagg gaagtccggg cggtcatcgg cccgaacggc
     5761 gcgggcaaga cgacgctgtt caatctgctg acgggtcacc tggcttgcga tcagggggcc
     5821 gtgctgttca aaggggaacg gatcagcggc ctgccgcccc accggatctg gcggcgcggg
     5881 atcagccgga cgtttcagat caccgccacg tttcggagcc tcagcgtcct gcagaacgtc
     5941 caggtagcga tcctctcgca tcgggggcga agccgggaga tgttctcgat cacccgcagg
     6001 ttagtccggg agcctgccgc agagctgctg gaacaggtcg gactgcaggg gctggcggaa
     6061 cgcgcctgcg gcgctctctc ctatgcggat ttgaagcggt tggagctggc cctggccctg
     6121 gccaacgatc cggaagtgct gctgctggac gaaccgacgg caggcatggc cccgcaggag
     6181 cgcatggacc tcatggccct ggtcttgaag accgtggccg agcgcggcct gggcgtcctg
     6241 ttcaccgagc acgacatgga tgtggtcttc gctgcggccg ggcggatcct ggtgttgcat
     6301 caggggcgaa tgatctctga gggaacgcct caagaggtcc gggcaagtcg cgctgttcag
     6361 gagatttact tgggcggtcc ggctcagacg gatggctggt aaatgacggc tgacgaaaag
     6421 aagatgctcg aagtcgcgga cctcgaagcc tactacggcc cgagccacat cctgtccgac
     6481 atctgtctcc acacaggccg aggcgaggtt gtggcgcttc tcgggcgaaa cggggcgggc
     6541 aagtccacca cgctcaaaac catcatgggg ctcgtccctc ccgcagccgg tcgagtgcgc
     6601 ttccaggggg aagacctcgc gggacagccg ccctatgcca tctgcaggcg ggggatcggc
     6661 tttgttcccc aggagcggcg catcttcccc gatctgacag tcggtg
//
LOCUS       DBNGZC010000469         8450 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_431570, whole genome shotgun sequence.
ACCESSION   DBNGZC010000469 DBNGZC010000000
VERSION     DBNGZC010000469.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 8450)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8450)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8450
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_431570"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="AC16G0_08230"
     CDS             complement(<1..234)
                     /locus_tag="AC16G0_08230"
                     /inference="COORDINATES: protein motif:HMM:NF015444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCC domain-containing protein"
                     /protein_id="HIR5099640.1"
                     /translation="MQYFVSKTSPRAILIMFDRGDLLLEGLQEVVKKEKIDTAAITGG
                     IGSLSRVHLHTITTTGFPTSDKYWNFTGAIELAS"
     gene            complement(268..1116)
                     /locus_tag="AC16G0_08235"
     CDS             complement(268..1116)
                     /locus_tag="AC16G0_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5099641.1"
                     /translation="MRRKRLRTLQRILGVYLPLAAFLCFALFPFYWMLISSFKANPEL
                     YNLRANRLWIKNPTLDQFRDLFAKTAIIRWLANSFFVSFLTAFISMTLGVMAGYALAR
                     LKFPGAQAFGLATFITYLVPTTLIFIPLARVVQSLGLSNSIWSLVVTYPTFLIPFCTW
                     MLIAYFKSIPKDMEECAMIDGCSRIGAMIRIVLPMAVPGVVFSAMFGFTLSWNEFVYA
                     LTFISNTAEKTMSVAVPTELIRGDAYFWGELMAASLLGSVPVALLYSFFMDYFVSGLT
                     AGALKG"
     gene            complement(1325..2242)
                     /locus_tag="AC16G0_08240"
     CDS             complement(1325..2242)
                     /locus_tag="AC16G0_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5099642.1"
                     /translation="METVAEVKYVPAQPRLSRFLKPEALTGYFLISPAVFLMLVLLAY
                     PFVLAVYISLTDRVLGEPGKFIWFRNFYKLVQDPLFRQTVWNSFVYTIATVFFKMLLG
                     VILALLLNQEIRCRNLIRGAILLPWIVPTSLSVLTWLWMFDSLFSVVNYILLGVGLIS
                     TKIQWLGDPFWAMVSVIIVNTWRGLPFFAVSFLAGLMTIPKELYEAAETDGALRFRQF
                     WHITLPLLQPVIAVVVLFSTIWTFADFQIVYILTHGGPINATQIFATMAYDVALVAGR
                     IGEGSAISLFLIPALLVVIILMLRYLRKE"
     gene            complement(2270..3589)
                     /locus_tag="AC16G0_08245"
     CDS             complement(2270..3589)
                     /locus_tag="AC16G0_08245"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099643.1"
                     /translation="MRERKKRAAHAGGDRTISRRRFLGVTAGATAALSFGTFIRSGLG
                     AAKINVLTWSNTLNAMDDTLREQAKQYTKEKGVEVTFEFINQTDLPTKTAAAVESGAG
                     PDIITTWSDGAHLHDKALIDMSDIADSLGKQLKGWTNIGRDIGFVGKVWKAVPWGIIP
                     ICVTYRSDWCKEVGYEKFPESYDDLLVMSKKLKDKGHPVGFALGRAIGDGNQSHYPIF
                     WAFGGTECKKDGKTVAINSPETAKAVDWRRRLFKEAETEDVLSWDDSSNNRAFLAEAV
                     SVTGNAASIYWAEKKNAPQLRDVTNHALWPKGPAGRFGYSTAMGLGIFKFSKFQKEAK
                     AFITWLMQPTQYSAWIGVGEGQIAGLANFYEDDPVWKKDPKLEVIREIPKYVRMPGYP
                     APPSRPAAEAMVKFVLVNMFARACKGMSTAETIKIAESELKEIYGKA"
     gene            complement(3726..4778)
                     /locus_tag="AC16G0_08250"
     CDS             complement(3726..4778)
                     /locus_tag="AC16G0_08250"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR5099644.1"
                     /translation="MPPRPPKVTRLMDVARRAGVSTATVSRVLNDRGPMSDLARDRVL
                     RAVRELDYHPNWLARSLRSRRTDTLGLVVPDIENPFFTALINGVEREASTRGWNVLLG
                     NTDEGIQKEESVVRALVERRIDGLLLCPSAGPHDYLGRYVDRRLPIVTVNRALREFPL
                     PRVISDNERGAHEAASHLLAKGLQPLALILGTPDLSTTQARLAGCRRAITEFGLGQDA
                     LYLAVGYGRTEEGYKAALKCLEATPRPRAIFAFNNLMAESALMAIHAGGVRCPEEVAL
                     IGFDDFRSAAALSPPLSVVEQDPVGMGVKAVEVLAQVIESGQPAEPETVMPTRLVSRA
                     SCGCTVTPGGGTTPAP"
     gene            complement(5013..6107)
                     /locus_tag="AC16G0_08255"
     CDS             complement(5013..6107)
                     /locus_tag="AC16G0_08255"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR5099645.1"
                     /translation="MKGKAAIWWEAHKPLEIREYPVPDVKPDGILVKLSLANICGSDI
                     HFVEGRGPRLKGGIPQILGHEMMGTILKRGSEVATDSLGQPLQEGDRLVFSYYSPCGQ
                     CWACYTGVPGCTNRYRRWIGVPADKPPHFNGGFAEYYYLHPGHWRFKVPPELPDHVVS
                     PVNCALSQMIYTLHKVGVMLGDTVVIQGAGGLGLYGAAVAKEMGAGKVISLDKRDHRL
                     ELAKAFGADVTINVEKVPRERRLETIQDLTGGVGADLVVEVCGSPAAVDEGLRMARVG
                     GRYALIGNINLDMPDQVDPGNAVRFSKTITAISVYAQWVIPRALDLLVRCKDKYPFHQ
                     VISHTFPLEEINKAFDFARTGQPIRVALEC"
     gene            complement(6211..6870)
                     /locus_tag="AC16G0_08260"
     CDS             complement(6211..6870)
                     /locus_tag="AC16G0_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5099646.1"
                     /translation="MLEYEGVQITWMGHDGFKFKKESVTYVDPFKLGTKPEAADLVCV
                     THEHFDHLSVDDLKKVVTQKTTVVTIAACQEAVKALRLKALRVVRPGDRLEVGQVQVE
                     VLPAYNTNKFRSPGNPFHPQVDGKVGFILTIGGIRIYHAGDTDEIPEMAKAKGVDIAL
                     LPVSGTYVMTAEEAIKACNAIQPKLALPMHYGAIVGSEKDAEAFKQGVKCRVEILTPE
                     H"
     gene            complement(6979..7239)
                     /locus_tag="AC16G0_08265"
     CDS             complement(6979..7239)
                     /locus_tag="AC16G0_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099647.1"
                     /translation="MSPRAEERCPWEDTCPFARMTQVGEDIRAGLSELKRRLPKGFRE
                     HAMAAERETLLAMRTLLDGLLDAMQSKTPGPEKGRGRKNKID"
     gene            complement(7223..>8450)
                     /locus_tag="AC16G0_08270"
     CDS             complement(7223..>8450)
                     /locus_tag="AC16G0_08270"
                     /inference="COORDINATES: protein motif:HMM:NF014433.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HIR5099648.1"
                     /translation="TRTVRSASMRIILYTGKGGVGKTTVAAATAFWAAHLGHRSLVIS
                     TDPAHSLGDAFDKPLGAEPSAVCKNLWAQEINVLEEIQKYWGEVQEYLTALFATRGVD
                     AIVAEEMAVFPGMEEVCSLLSVWQHVQAGHYDCLIVDCAPTGETMRLLSFPDIARWYM
                     DKLFPWERRIVTRLGPVVQPLVPVPLPKDNVYAALEGLFHKVDETKSLLVDTQRSSIR
                     LVLNPEKMVIREAQRALTYLNLYGYAADAIVSNRVLPEESTKGYFAEWTGIQRRYRKM
                     IQEAFAPLPVWEIPFFKTEVVGTRMLALMAETLFGNRDPTQVFFSGSVQEVERRGKEY
                     VFRLKLPFLRKEEVDLFQRGEELTVSIGNFRREIVLPRSLANLRVLGAKAEEGALVIR
                     FAPREGRGGKGDVTAS"
BASE COUNT         1533 a         2738 c         2625 g         1554 t
ORIGIN      
        1 gctcgccaac tcgatggcgc cggtgaagtt ccagtacttg tccgaggtgg ggaagccggt
       61 ggtcgtgatg gtatgcaggt gcacccgaga cagcgagccg atcccgccgg tgatggcggc
      121 ggtgtcgatc ttctcctttt tcacgacttc ctgcagtccc tccagcagaa gatcaccccg
      181 atcgaacatg atcaggatgg ctcgcggcga cgtcttggac acgaagtact gcacgggaat
      241 cctcctttcg gggtgggagg cggtgcgtca acccttgagg gcgccggcgg tcaggccgga
      301 gacgaagtag tccatgaaga aggaatagag caacgccacc gggaccgaac ccaggagcga
      361 ggcggccatc aattcgcccc agaaataggc atcccctcgg atgagttccg tggggacggc
      421 gaccgacatc gtcttctcgg cggtgttgga aatgaacgtc aaggcataca cgaactcgtt
      481 ccaggaaagg gtgaagccaa acatggcgga gaagacgacc cccggaacgg ccatgggcaa
      541 cacgatccgg atcatggccc cgatgcggct gcacccgtcg atcatggcgc actcctccat
      601 gtccttgggg atggacttga agtaggcgat gagcatccag gtgcagaacg ggatcaggaa
      661 cgtgggatag gtcacgacca gcgaccagat ggagttggat agacccaggg actggacgac
      721 cctcgccagg gggatgaaga tgagggtcgt cggcaccaga tacgtgatga aggtggccag
      781 gccgaaggcc tgggccccgg gaaacttcag gcgcgccagg gcatacccag ccatgacccc
      841 caaggtcatc gagatgaacg cggtgaggaa cgacacgaag aagctattcg ccagccagcg
      901 gataatggcg gtctttgcaa agaggtctcg gaactgatcc agggtcggat tcttgatcca
      961 cagccgattg gcgcgcaagt tgtaaagctc cggattggcc ttgaagctgc tgatcagcat
     1021 ccagtaaaag ggaaacaggg cgaagcacag gaatgccgcc agagggaggt acactcccag
     1081 gatgcgctgg agcgtccgga gccgtttgcg ccgcatgctc atcccctact aggctgctga
     1141 aaaaggccca tctgctggga cccatcctcc ggacgggtac cccgcgcgct cgcgcggcgc
     1201 tgcggcgtac cgaccaagta cgcctcgcac ctgcacccat cctgctggac gggtacccgg
     1261 cgcgccgcct tgcatctgga cctttttgag cagcctgagt aagaacggat ttttcgccat
     1321 cctgctattc tttccgaagg tatcgcagca tcaggatgat gacaaccaat aatgccggga
     1381 tcaagaacag cgagatggcg gatccttcgc cgatccgccc ggccaccaag gccacatcat
     1441 aggccatggt ggcgaaaatc tgggtcgcgt tgatcggacc gccgtgggtc aggatgtaga
     1501 cgatctggaa gtcggcgaag gtccagatcg tggagaagag gacgaccacg gcgatcaccg
     1561 gctgcagcag tggcagcgtg atgtgccaga actggcggaa gcgcagcgcc ccatcggtct
     1621 cggccgcttc gtagagttcc ttggggatgg tcatgagccc cgccaagaac gagaccgcga
     1681 agaagggcaa gccccgccag gtgttgacga tgatgacgga gaccatggcc cagaacggat
     1741 cgcccagcca ctggatcttc gtcgagatca gccccacgcc cagcaggatg tagttcacga
     1801 cgctgaagag ggagtcgaac atccagagcc aggtgaggac ggagagcgac gtgggaacga
     1861 tccaggggag gaggatcgcc ccccgaatca ggttccgaca gcggatctcc tgattcagta
     1921 ggagcgccag gatcacgccc aggagcatct tgaagaacac cgtcgcgatg gtgtagacaa
     1981 aagaattcca caccgtctgg cggaacaacg ggtcctggac gagcttgtag aagtttcgga
     2041 accagatgaa cttccccggc tcccccagga cccgatcagt caagctgata tacaccgcca
     2101 ggacgaaggg ataggcgagg agcaccagca tcagaaacac cgccggcgag atgaggaagt
     2161 atcccgtgag cgcctcgggt ttgagaaagc ggctcaggcg tggttgcgcc ggcacatact
     2221 ttacttcagc gacggtctcc atcggtgcct ccgccccggc ggcgtgcggc taggcctttc
     2281 cgtagatctc cttcagctcg gattccgcaa tcttgatggt ctcggccgtg gacatgccct
     2341 tgcacgcccg ggcgaacata ttcacgagaa cgaacttgac catggcctcg gcggcgggcc
     2401 gggacggcgg cgcagggtat ccaggcatcc gcacgtattt cgggatctcc cggatgacct
     2461 ccagcttggg atccttcttc cacaccggat cgtcctcgta aaagttcgcc agtccggcga
     2521 tctggccctc gccgaccccg atccaggcgg agtactgcgt gggctgcatc agccaggtga
     2581 tgaacgcctt cgcctccttc tggaacttgg agaacttgaa gatccccagt cccatggcgg
     2641 tagagtatcc gaagcggccc gccggcccct tcggccacag ggcgtgattc gtcacgtcgc
     2701 ggagctgcgg ggcgttcttc ttctcggccc agtagatgct ggccgcgttc cccgtcaccg
     2761 agacggcctc cgccaggaag gcccggttgt tgctggagtc gtcccaggag aggacgtctt
     2821 ccgtttcggc ctccttgaag aggcggcgcc gccagtccac cgccttggcg gtctcgggcg
     2881 agttgatggc cacggtcttc ccgtccttct tgcactccgt tccgccgaag gcccagaaga
     2941 tcggatagtg agactggttc ccgtccccga tggcgcgacc cagggcgaag ccgacggggt
     3001 gtcccttgtc tttcaatttc ttggacatca cgaggaggtc gtcgtagctc tccgggaatt
     3061 tctcgtatcc gacctccttg caccagtcgc tgcggtaggt gacgcagatg gggatgatcc
     3121 cccaaggcac agccttccag accttcccca cgaagccgat gtcccgcccg atgttggtcc
     3181 agcccttcaa ctgcttgccc aggctatcgg cgatgtcgct catgtcgatc agggccttgt
     3241 cgtgcaggtg cgccccgtca ctccaggtgg tgatgatgtc tgggcccgcc ccgctctcga
     3301 cggcggcggc ggtcttggtc gggaggtcgg tctgattgat gaactcgaag gtgacctcca
     3361 ctcccttttc cttggtgtac tgcttggcct gctctcgcag ggtgtcatcc atggcgttca
     3421 gagtgttgga ccaggtcaag acattgatct tggccgcacc cagcccgctc ctgatgaacg
     3481 tcccgaagga cagggcggcg gtggctccgg ccgtcacacc cagaaacctg cgtcgactga
     3541 tcgtccggtc gccgccagca tgtgccgctc tcttctttct ctctcgcatg gttgtctcct
     3601 ctccctgaat ggggttcagt ggacccaggc acccggcact ccacgcactt cgggtggaga
     3661 ccttgtgccg tacgctgtgc tcgggcgctc ctccggcacc caagcgcgtc gagccttgct
     3721 cgtggtcatg gagccggcgt ggtccctccc cccggcgtga ccgtacaacc gcaggaggcg
     3781 cgactcacga gacgggtggg catgaccgtt tcgggttctg ccggctggcc ggactcgatc
     3841 acctgggcca gcacctcgac cgctttcacg cccatcccga ccggatcctg ttccacgact
     3901 gacaggggcg ggctcagggc cgcggcagag cgaaagtcgt cgaagccgat gagtgccacc
     3961 tcctcggggc atcgcacgcc gcccgcgtgg atggccatga gggccgactc cgccatcaga
     4021 ttgttgaagg cgaaaatcgc ccgggggcgc ggggtggcct ccagacattt cagtgcggcc
     4081 ttgtacccct cctcggtcct gccgtagccg accgcaaggt ataacgcatc ctgccctagg
     4141 ccgaactcgg ttattgcccg gcgacagccg gccaaccggg cctgggtggt gctcagatcg
     4201 ggcgtcccca ggatgagggc cagcggctga agccccttgg ccaagaggtg gctcgccgcc
     4261 tcgtgggcgc ctcgttcgtt gtcggagatc accctgggaa gggggaactc ccggagcgcc
     4321 cggttcacgg tcacgatggg taggcgccgg tccacataac gcccgaggta gtcgtggggc
     4381 ccggcgctgg ggcacagcag caggccatcg atcctgcgtt cgactagcgc ccggacaacc
     4441 gactcctcct tctggatacc ctcgtccgtg ttgcccagca ggacattcca cccccgcgtg
     4501 gaggcctcac gctccacgcc atttatcagc gccgtgaaga acggattctc gatgtcgggg
     4561 acgaccagac ccagcgtgtc ggtgcggcga ctcctgaggc ttctggccag ccaattgggg
     4621 tggtagtcga gttcccgcac ggcccgcagg acgcgatcgc gggcgagatc gctcatcggg
     4681 ccccggtcgt tgagaacgcg tgagacggta gccgtggaca caccggcgcg tcgggcgaca
     4741 tccatgaggc gagtgacctt gggcgggcga ggaggcatgg cgcgattcca cgaaaccggc
     4801 ggcgacgagg gttgcgtgtc ggaaagctaa tggcacccgt cctcagggaa ccatgtaatc
     4861 cattacaccg gggtatatat atgaaaccgg tgcttcccgt caatgacaga tccattccat
     4921 gtcaccctta tctgacctct cggcgtcagg ctgctgatac tcgccagggc gaggcgtggt
     4981 cggctgccgg tcgcagaagc cttcgaacaa gatcagcatt ccagggcaac ccgaatgggc
     5041 tggccggtcc gggcgaagtc gaacgccttg ttgatctcct ccagggggaa ggtgtgagag
     5101 atgacttggt gaaaggggta cttgtccttg catcgcacca gcaggtccag ggctcggggg
     5161 atgacccact gggcgtagac cgatatggcg gtgatcgtct tgctgaaacg cacggcgttg
     5221 cccgggtcca cctggtcggg catgtccaga ttgatgttac cgatcagggc gtagcgaccg
     5281 cccactcgcg ccattcgcag gccctcgtcc acggcagccg gagacccgca cacctccacc
     5341 accaggtcgg cgcccacgcc gcccgtgagg tcttggatcg tctccaggcg ccgctcccgt
     5401 ggcaccttct ccacgttgat ggtcacatcg gccccaaagg ctttggccag ctccagacga
     5461 tggtcccgct tgtccaggct gatgaccttg cccgccccca tctccttggc cacggcggct
     5521 ccgtacaggc caagacctcc ggccccctgg atcaccaccg tatcccccag catgaccccg
     5581 actttgtgca gggtgtagat catctgggag agggcgcagt tcaccgggga gaccacatgg
     5641 tccggaagct ccggcggaac cttgaacctc cagtggcccg gatgcaggta gtagtactcg
     5701 gcgaaaccgc cgttgaagtg aggcggcttg tctgcgggca cgccgatcca ccgccggtac
     5761 cggttggtac aaccgggcac gcccgtgtaa caggcccagc actgcccgca ggggctgtaa
     5821 taggagaaga ccaggcgatc cccctcttgg agcggctggc ccagggaatc ggtcgcgacc
     5881 tcgcttccac gcttcaagat cgtccccatc atctcgtggc cgaggatctg ggggatgcct
     5941 cctttgaggc gcgggccgcg cccctccaca aaatggatgt cggaaccgca gatattggcc
     6001 agactcagct tgaccaggat cccgtccggt ttcacgtccg gcaccgggta ctcccggatc
     6061 tccagtggct tgtgcgcctc ccaccagatg gccgcctttc ccttcatgga ctcccctccc
     6121 ctgatggact gacaggggca gcgacagcgg tacacggcac gcgcaccgct cctcccccct
     6181 gtccctgtcg gaattccccg tcgtcctctt tcagtgctcc ggcgtgagga tctccacccg
     6241 gcacttcacc ccctgcttga atgcctcggc atccttctcc gaccccacga tggccccgta
     6301 gtgcatgggc aacgccagct tgggctggat ggcattgcag gccttgatcg cctcttccgc
     6361 ggtcatcacg taggttccgc tgaccgggag gagcgcaatg tccacaccct tggccttggc
     6421 catctccggg atctcgtcgg tgtcgcccgc gtgataaatc cgaatgcccc caatcgtgag
     6481 aatgaatccg accttcccat ccacctgggg atggaacggg tttccagggg accggaactt
     6541 gttggtgttg tacgccggca gcacctccac ttgcacctgg ccgacctcca ggcggtcccc
     6601 cggtcggacc acccgcaggg ccttgaggcg gagcgccttg acggcctcct ggcacgcggc
     6661 gatggtcacc accgtggttt tctgcgtcac caccttcttc aggtcgtcca cgctcaggtg
     6721 atcgaagtgc tcatgggtca cgcagacaag gtcggccgcc tcgggcttgg tgcccagctt
     6781 gaacggatcg acgtacgtga cgctttcctt cttgaacttg aagccgtcat ggcccatcca
     6841 cgtgatctgc actccttcat actccagcat gattcacctc ctgccggcgg tcggatcacc
     6901 cggaggccga gagcccgagg cccagccgct cccggatgtc gccggccggc cctgccaccg
     6961 gcgttggctg cttgtggttc agtcgatttt gttcttgcgc cctctcccct tctctggccc
     7021 tggggtcttg gattgcatgg cgtccagcag tccatccagg agcgtccgca tggccaagag
     7081 cgtctcccgc tccgcggcca tggcgtgctc ccggaatccc ttggggagtc gtcgcttcag
     7141 ctcggacagg ccggcccgga tgtcctcgcc cacctgcgtc attcgggcaa acggacaggt
     7201 gtcctcccag gggcagcgct cctcagctcg cggtgacatc ccctttccct cctcgcccct
     7261 cccggggggc aaaccgaatg accagcgcac cctcctccgc cttggctccc aagacccgca
     7321 ggttcgccag ggagcgcggc agcacgatct cccgccggaa gtttccgatg gagacggtga
     7381 gttcctcccc gcgttggaac aggtccacct cctctttccg gaggaacggc agcttcagac
     7441 gaaagacgta ctccttcccg cgtcgctcga cctcctgaac cgaccctgag aagaacacct
     7501 gggtcgggtc ccgattgccg aatagggttt cggccatcag ggccagcatc ctcgtgccca
     7561 cgacctcggt cttgaagaag gggatctccc acaccggcag cggtgcgaag gcttcctgga
     7621 tcatttttct gtaacgccgc tggataccgg tccactcggc gaagtagccc ttggtcgact
     7681 cctcgggcag gacccggttg ctcacgatgg cgtctgcggc gtagccgtac aaattgagat
     7741 aggtgagggc gcgctgcgcc tccctgatga ccatcttctc cgggttcagg accagccgga
     7801 tggaggagcg ctgagtatcc accagcaacg acttggtttc atccaccttg tggaagaggc
     7861 cctccagggc cgcgtagacg ttgtccttgg gcaaggggac cgggaccaag ggctggacca
     7921 cggggccgag gcgggtcacg atccgccgct cccaggggaa cagtttgtcc atgtaccagc
     7981 gggcgatgtc gggaaagctg agcagccgca tggtctcacc cgtgggggcg cagtccacga
     8041 tcaggcagtc gtagtgcccg gcctggacat gttgccaaac cgagagcagg ctgcacactt
     8101 cctccatgcc ggggaaaacc gccatctcct cggccacgat ggcatccacc ccccgggtgg
     8161 cgaagagggc cgtgaggtac tcctggacct cgccccagta cttctggatc tcctccagaa
     8221 cgttgatctc ctgggcccag aggttcttgc acaccgcgct gggctccgcg cccaggggtt
     8281 tgtcaaaggc atcccccagg ctgtgggcgg gatcggtgct gatcaccagt gaccgatgac
     8341 ccagatgggc ggcccagaac gccgtggcgg cggccacggt cgtcttcccc acacccccct
     8401 tgccagtgta caggatgatc cgcatggagg cactccggac cgtgcgcgtg
//
LOCUS       DBNGZC010000470         2345 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1016928, whole genome shotgun sequence.
ACCESSION   DBNGZC010000470 DBNGZC010000000
VERSION     DBNGZC010000470.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1016928"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="AC16G0_08275"
     CDS             complement(<1..369)
                     /locus_tag="AC16G0_08275"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012549.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR5099649.1"
                     /translation="MTAEEILQKVKERFGDAVTEAEAKGVEVRLTVSPAKSWDVCRFL
                     RDLGFEYLNCLSGVDWLTRLEVVYDISSLTHPNKVHRRVPVSRENPEVRTTTDIWRGA
                     NWHERECYDLFGVRFDGHPDH"
     gene            complement(439..798)
                     /locus_tag="AC16G0_08280"
     CDS             complement(439..798)
                     /locus_tag="AC16G0_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR5099650.1"
                     /translation="MAADYAYVGFFLIFGVLFVVLTIEIMSRIVRPHHPTAQKISTYE
                     CGEEPVGIGWIRFHVRYYLYALIYIVFAVETVFLFPWAVVFRALGLFAFIEMMVFIGI
                     LLVGLAYAWKKGALEWV"
     gene            1559..>2345
                     /locus_tag="AC16G0_08285"
     CDS             1559..>2345
                     /locus_tag="AC16G0_08285"
                     /inference="COORDINATES: protein motif:HMM:NF013346.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HIR5099651.1"
                     /translation="MTAFVASLLFVTLAEMGDKTQLLAMAFATRFPAPTVLAAVFVAT
                     LLNHSLAVAAGRLLTTVIPLEAISLVAALSFILFGLWTIRGDSLEGEDQRRSAFGPFL
                     TVAIAFFLAEIGDKTQLATISLAVKYETPLAVLLGTTAGMVVADSIGIILGIVLGKRL
                     PDNLIRLVSAGVFIGFGFLGAGAMFPTWLPRAGTAVVLFAIGVATLGAAHALLIQRRR
                     SEAHPAVGRGPSRLPRRLTQTLFAGLLVVGWVAGLGLVKPLTAI"
BASE COUNT          396 a          809 c          716 g          424 t
ORIGIN      
        1 gtgatccggg tgtccgtcga agcggacccc gaagaggtcg tagcattccc gttcgtgcca
       61 gttcgcgccc cgccagatgt ccgtcgtcgt ccggacctcc ggattctccc ggctcaccgg
      121 gacgcgacgg tgcaccttgt taggatgggt gagcgacgag atgtcgtaca ccacctcgag
      181 ccgcgtcagc caatcgactc cgctcaggca attcagatac tcgaaaccga gatcccggag
      241 gaagcggcag acgtcccaac ttttcgccgg cgaaaccgtc aatcgcacct cgacgccctt
      301 cgcctccgcc tcggtgacgg cgtcgccgaa cctctccttg accttttgca ggatctcttc
      361 ggccgtcatc tcagtccccg gcaattcggc aatctggcaa ccaggccatc aggcgtgccc
      421 cgttgcttgg ttgccagttc agacccactc gagcgcgccc ttcttccagg cgtaggccag
      481 cccgaccaag agaatcccga tgaagaccat catctcgatg aaggcaaaca gcccgagcgc
      541 gcggaagacg accgcccacg gaaacagaaa gactgtttcc accgcgaaca cgatatatat
      601 gagagcgtaa agatagtacc gaacatggaa gcgaatccag ccgataccaa ccggctcctc
      661 gccgcattca taggtggaga tcttctgggc ggtcgggtgg tggggacgga cgatccgaga
      721 catgatctcg atggtcagga cgacaaacag gacgccgaaa atcagaaaga agccgacata
      781 ggcgtaatcc gctgccatgg aggccccctc tacgtcaccg ggaagcaggc agtgcgcgga
      841 tccagtaggc gagattggct cactgaggct acaaaccgcg agcgcctggc gtcacaaaaa
      901 aagcccccaa gtagggctcc gccgatctcg taaatcccgg aacaataatg cggacttcta
      961 gcatggggtc ccaggcctgt caagggaaat gctggacaag cgtcacccca gcagaccgag
     1021 gcgttccagc gccgccggcc gcgggcgccc ggccttccat cgccgtcttg gggtgttggc
     1081 cgctcgccct ttgtccgggc gcccggacgt gcggacgatg cctcttgcgc gacgggtggg
     1141 gcgcagctac tctcgccctt gccaaagggg agtagcaggg ccgtctcggc ggccccacgg
     1201 ccgccaacaa acacgacgca cggcttctgg cgtccatgac cggacccggt ccgggtgcaa
     1261 gagctttggt ggggtgagcc ccgtgtgggc gcgcccgacc ttgctttgca accggagggt
     1321 tcatggtcct ggctctttgt gcttcgcccc gtcggcgccc cgccttcgcc ccccaggctc
     1381 gagaagcgct gccccactcc gccacgcgac gcgcctccag gctcattgac cctgacggat
     1441 cccggcaggc atctctcggg cagtcttgcg cacctcgcgt cgcgtgtccg acaagcggct
     1501 gtcattttcg caccgctggt cgggcatccg cctttcgctc tcctggagag gatccagcat
     1561 gaccgccttc gtcgcctccc tcctcttcgt gactctggcc gagatgggag acaagaccca
     1621 gctcctggcg atggccttcg ccacacgctt tccggctcca accgtgctgg cggcggtctt
     1681 cgtcgcgacg ctcctgaatc attccctggc ggtggccgcc ggccgccttc tgaccacggt
     1741 gatcccgctg gaggccatct ccctggtcgc ggccctctcg ttcatcctgt tcggattatg
     1801 gacgattcgc ggggacagtc tggaggggga ggaccagcgg cggtcggcct tcgggccgtt
     1861 tctcaccgtg gccatcgcct tcttcctggc ggagatcggg gacaagacgc aacttgccac
     1921 catcagcctg gccgtcaagt atgagacccc cctggcggtg ctccttggca ccactgcggg
     1981 aatggtggtg gccgatagca tcgggatcat ccttggcatc gtcctcggca agcgcctgcc
     2041 cgacaacctg atccgcctcg tctcggccgg ggtgttcatc gggttcggct ttctgggcgc
     2101 cggggccatg ttcccgacct ggctcccgcg cgcaggaacc gcggtggtcc tgttcgccat
     2161 cggcgtcgcc acgctcggcg cggcccacgc cctcctgatc cagcgtcggc ggagcgaggc
     2221 gcacccagcg gtcggacgag gaccgtcccg gctgcctcga cgcctcacgc agacgctctt
     2281 tgccggactg ctcgtggtgg ggtgggtcgc cggtctcggc ctggtcaaac ccttgaccgc
     2341 catcg
//
LOCUS       DBNGZC010000471         3670 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_921017, whole genome shotgun sequence.
ACCESSION   DBNGZC010000471 DBNGZC010000000
VERSION     DBNGZC010000471.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_921017"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..1471)
                     /locus_tag="AC16G0_08290"
                     /pseudo
     CDS             complement(209..1471)
                     /locus_tag="AC16G0_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_145269244.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
     gene            complement(1534..2394)
                     /locus_tag="AC16G0_08295"
     CDS             complement(1534..2394)
                     /locus_tag="AC16G0_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005372295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HIR5099652.1"
                     /translation="MAQAQPRTSSRSAPPVDDLWRIPDVLWDRIVPLLPPRPHHPLGC
                     HNPRVDDRKAMDGIFFVLRTGCQWGALDATGICSHASAHRRFQEWTAAGAFVTLWAEG
                     LQEYDELNGLDWDWLALDGAMTKAPLGGERTGRNPTDRGKQGTKRSLLTEANGVPVGL
                     TVEGANRHDKILVEATLESIPVERPEPTEAKPQGMCLDKGYDYDDTRELVREFGFTAH
                     VRARGEEARALKREAGFKARRWVVERTHSWMNRFRRILIRLEKKVENYFGMLHFVCAW
                     ITYRCCGLLG"
     gene            complement(2484..>3670)
                     /locus_tag="AC16G0_08300"
     CDS             complement(2484..>3670)
                     /locus_tag="AC16G0_08300"
                     /inference="COORDINATES: protein motif:HMM:NF033540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HIR5099653.1"
                     /translation="KRLALMLARAGSDPKRGVLCLDDTAIPKKGEHSVGVQWQYCGEL
                     GKLANCQAIVTAHYTDDHTHWPIGMRLYLPEGWANDPARRRKTRVPEAIAFATKPELA
                     LALLDRAREAGVRHAVVTADCGYGDIPDFLAGLEGRREPYIVQVSKAFGVRLSEEVAA
                     AASNPVPLGRRPGRPRKDGTTSEEPYTRSGRPRKHPHPVQVAPLHQAQAVTGSLPESA
                     WRTVTVLDGSEESCQRLGCRIRVHRGHDDVTGPEGWLIGERPLPGQPGDPKWYFAWGL
                     DRRSLERQLRFGHRRWSIERLHQDAKQELGLGDYQGRMWPGLHRHLALVGLIWCYAVL
                     GAASATPEVSGAFSPSAEPARGAASGAGGAGFDGYLPGLPQSHRAADDGLPPPRPFTL
                     RR"
BASE COUNT          627 a         1235 c         1176 g          632 t
ORIGIN      
        1 cctccggagc ggacgccgag ttccggtacc ggaaggcggc ctgatcccac ttatgggaca
       61 gtcactaata ctactccgtt aaccttgata tgaatccgat tgctaaatgg taatgcgggt
      121 tgcagtactc gcaatccggt ttggcccgcg ggctcagcaa ttcctgaagc gcccggcgga
      181 ttgccggcac cgtgatccgg ggcacctccc cacttttttt ccccggcaac gccggagcct
      241 ccttctcgcg cagttgttcc aacaccagaa atccatacgc cagcatgacc aggcaggcat
      301 ggtgatgaaa cccacgccac aaccggccct cgaaatgatc caggcccaat tcttccttca
      361 tctgttggta gccttgctcc accggccagc ggctcttcca cagccggacc gccaggagcc
      421 ggctggttcg tgcgggcagg ttcgagaagg cgaaccgaat ctcgccgtcg gcctgttctt
      481 cgatcaacag ccagatcggc tcggcgccgg cgcagtcgcc ccgctcccag ccctgggccg
      541 gccagacccg gatccaactg aatcggcccg acaacttgcc tttcgtcccc tcacgccagg
      601 tcactttccg gcggggcaag cgctgggcca gtgcccccag gctgaccggt cgcgggccct
      661 ccgcggccag ccgcggtttg gtccgtggtc ggcccttggt cgaaggggtg ggctgctccc
      721 agcggggttt ctcgctgaag accaccatct ccgaagtgac ccccgcgagg tagaacaatt
      781 gcctctgggc cagaccctcg cgaaacggct gcgagacgcc atagcccgca tcggtgatga
      841 ccacgttgcc gggaagcagg ccctcactcc gaacccggtc gagcagctcc agggcgattt
      901 gccccttggt cttctcctcg gggcacgatg cggggacttt ggccgcctgg agccgcttgg
      961 gagatgctgt ccaggatggg ggcagataca gccgcaaggc ggcggggaag tgtccctggg
     1021 ggctgacgta gtggacggtg acggcgacct ggcagttggc cttcttcccc aacgggccgc
     1081 agtattggtg ctgcacgccg acggagtgct tgccctgctt ggggaagccg gtatcgtcga
     1141 tgacgaagat cccccgagga ctggccaggc ggcgagccat gacaaagcgg tagcgcttcc
     1201 agacggccag ttcgtcccag gggctctggc tgacgaattg ttgcagggct tgttcggggt
     1261 ctttgatgtg ggagagttcc gcgggcaagg ggacgcgggt ggccatcggt tcgatgcttt
     1321 tgcgttcgcc gttttggagc aggccgcgga ggtagacgcc actccaggag acttgggacg
     1381 gaaagcggaa gaggtctcgg aactgatcag cgtagtcccg aagccgaagg aggacttcgg
     1441 gggcgagttc gggggtgtag gtcttgttca taatgttcat acgaacaaaa cccccgaaag
     1501 gttcgtagga ggttaacgga gtagtattag agcctatccc agtaggccac agcatcgata
     1561 cgtaatccag gcacacacga aatgcaacat accgaagtaa ttctccacct tcttctccaa
     1621 acggatcagg atccgacgga agcgattcat ccaactgtgg gtccgctcca ccacccagcg
     1681 ccgcgccttg aaccccgcct cccgcttcag cgccctggcc tcctcgccgc gggcccgcac
     1741 atgcgccgtg aagccgaact cccggaccaa ctcccgcgtg tcgtcgtaat cataaccctt
     1801 gtccaggcac attccctgcg gcttcgcctc ggtcggctca gggcgctcca ccgggatgct
     1861 ctccagcgtc gcctcgacca ggatcttgtc atgccgattg gccccctcga cggtcaaccc
     1921 caccggcacc ccgttggcct ccgtcaacag gctccgcttc gtcccttgct tgccccggtc
     1981 ggtcgggttc ctcccggtcc gttccccgcc gagcggggcc ttggtcatcg ccccgtccag
     2041 cgccagccag tcccaatcca ggccattcaa ctcgtcgtac tcctgcagcc cttcggccca
     2101 gagggtgacg aaggcgccgg cggccgtcca ttcctggaag cggcggtggg ccgaggcgtg
     2161 ggaacagatg ccggtggcat cgagggcgcc ccactggcat ccggtgcgga ggacgaagaa
     2221 gatgccgtcc atggccttgc gatcatcgac gcgggggttg tgacaaccca gcgggtggtg
     2281 gggtcgtggc ggcagcagtg ggacgatccg gtcccacaac acgtcaggga tccgccagag
     2341 atcatcgacg ggaggagcgg atcgggacga cgtacggggc tgagcctggg ccatcagaca
     2401 catctccggg gaacaggcga acatcacacc ggaattgtag gcgcccgtcc acctaccact
     2461 actttggcga cataatcgga acatcatctt ctgagtgtga atggccttgg cggcgggagg
     2521 ccatcgtcgg cagcacgatg cgactgcggc aggccgggca ggtaaccgtc aaagccagcg
     2581 ccgccagcac ctgacgccgc gcctctggca ggctccgccg agggggaaaa agcccccgac
     2641 acctcagggg tggcggaggc cgctcccagc accgcatagc accagatcag gcccaccagc
     2701 gccaggtggc ggtgcaatcc cggccacatc cggccctggt aatcgcccag gccgagctcc
     2761 tgtttggcat cctggtggag gcgctcgatt gaccagcggc ggtggccgaa ccgcagttgc
     2821 cgctccagcg agcggcggtc cagaccccag gcgaaatacc acttcgggtc acccggctgc
     2881 cccggcagcg gtcgctcgcc gatcagccaa ccctccgggc cagtgacgtc gtcatggccc
     2941 cgatgcacgc ggatccggca ccccaggcgc tggcaggatt cctccgaccc gtccagcacg
     3001 gtgaccgtcc gccacgccga ctcgggcagg gaaccggtca cagcctgcgc ctggtgcagc
     3061 ggcgccacct ggactgggtg gggatgcttt cgcggtcggc cgctccgcgt atacggctcc
     3121 tccgaggtgg tcccgtcctt gcgggggcgt cccgggcgac ggcccagcgg gaccggattg
     3181 ctcgccgcgg cggcaacttc ctcggagagg cggaccccga aggccttgct gacctggacg
     3241 atgtacggct ctcgtcgccc ttcgagcccg gccaggaagt cggggatatc gccgtagccg
     3301 caatcggctg tcaccacggc atgcctcact ccggcctcgc gggcgcgatc cagcaaggcc
     3361 aaagccagtt ccggcttggt ggcgaacgcg atggcctcag gcacccgcgt cttcctgcgg
     3421 cgggccgggt cgttggccca tccctcgggc aggtagagcc gcatgccgat gggccagtgg
     3481 gtgtggtcgt cagtgtagtg tgccgtgacg atggcctggc agttggccag cttgcccagc
     3541 tcgccacagt actgccactg cacgcccacg gaatgctcgc ccttcttggg gatcgcggta
     3601 tcgtccaggc agagcacacc ccgtttgggg tccgaaccgg ctcgcgcgag catcagggcc
     3661 aggcgctttt
//
LOCUS       DBNGZC010000472         4407 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1900993, whole genome shotgun sequence.
ACCESSION   DBNGZC010000472 DBNGZC010000000
VERSION     DBNGZC010000472.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1900993"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="AC16G0_08305"
     CDS             complement(<1..176)
                     /locus_tag="AC16G0_08305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HIR5099654.1"
                     /translation="MDRREFLTLASATGLSLGAGLWTPAAAASAPATAQPKRGGTLKF
                     PVDANVTPWPPIGLL"
     gene            complement(265..2058)
                     /locus_tag="AC16G0_08310"
     CDS             complement(265..2058)
                     /locus_tag="AC16G0_08310"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR5099655.1"
                     /translation="MAHFPAEEQAFVDAVSTKRLMESTATIAQWVRLSGTPEEAHAFD
                     WLEAQLKWYGLEVERYSFPALVSWPESASLHITTADGRTEEIICATHGFAASTPEEGL
                     EGELVYVGRGTEEELAGRDVRGKIVLVDGIAAPNRGLVLEAAGVAASIWVAGQRLHER
                     LMSPVWGTPTPETAHLLPRTPSISIKGLDGARIREAMRTGTVRVKLLTRVFQGWKDIP
                     CLTGDLRPTTDRPEAHTFIMLSGHVDSWYYGAMDNGSANATQLEVVRLLAAHRSSLRR
                     GLRVAFWSGHSHGRYAGSARYADHKWQDLHDNCAAHVNVDSVGGKGATVLTEGNSMAE
                     MRGFASEVIQAVAGQQLSPRRFGRSGDQSFWGHGIPSLLMTLSEQPPEAADPVLAALF
                     RQISGGHGKSGGLGSWWHTPEDTLDKIDPEFLRRDAIIYTLLTYRLCASTVLPLDYLA
                     VVDELKAVVDDLQAKAGDGFDLSPAAEQLAALGSAVKRLQGRIKALQQGRVSDKAARV
                     VNRCLMELGRALIPVNYARSGQFDQDLAVPTQPLPGLQPVAKLAAMERASDQRQFLIT
                     RLARERNRLVHSVISAVRSVEAALSALEREI"
     gene            complement(2456..3391)
                     /locus_tag="AC16G0_08315"
     CDS             complement(2456..3391)
                     /locus_tag="AC16G0_08315"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR5099656.1"
                     /translation="MNRVLAVVAASVCLCVLPAARAEGGSMTEPARPLKVVTLNLYHG
                     GPFSGLLGDGQQLDRRLEMVARELRVLDPDIIGLQEASESRQRGNVAARLAAQLGLQY
                     VFAAANPRPFESSRVNRLIASLLNFSEGPAIVTRFPIAAWESLDLPRCGSYADSRLLL
                     HARLQSPWGGLDVFSTHTIGDPCQTGRVAQLVRNRRGPFPSVLMGDFNAPEDSVAIAA
                     ITQKGGLVDAFRTVNPFEPGLTVWQRIDAPASMVKRRADYLFVCPGTEFPGKILSSRV
                     VLNRPQQLQDGTYLWPSDHYGVLARLEIFPPDRNP"
     gene            complement(3626..>4407)
                     /locus_tag="AC16G0_08320"
     CDS             complement(3626..>4407)
                     /locus_tag="AC16G0_08320"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5099657.1"
                     /translation="GKLFGIGMTSSSRSTDTIYNFEQYLLAFHGDVITPDGKVVAGEA
                     KNREAIIKTLSFFTSIYKEGYVPPDALTWTDADNNVNFNNRTVVMTPNPSISIPGSWF
                     FTNKDYYYNKIGSILQPTSPIDGKGYPYLVAVKQIIIPKDAKEPALAKKFIKYVLETR
                     HFTDYVKASNGRWFPSFKDVAADKFFHDPKDPHVSLATEQHSKYPTRPFGFSYHPAYV
                     QVYTENVWGKAISRVVTEKWSNEQAVDEAVTRIETIFKNWK"
BASE COUNT          766 a         1427 c         1423 g          791 t
ORIGIN      
        1 aggagtccga tgggcggcca gggagtcacg ttggcatcca cggggaactt gagcgtgccg
       61 ccccgcttgg gctgggcggt cgccggcgcc gaggcggcag cggccggggt ccataacccc
      121 gcccccagac tgagccccgt ggcggaggcc agcgtgagga attcccgtcg atccatggaa
      181 cgtccttgcc cactgcgcag cccgcgattc ccctgatggc tcacggcctg actcctttcc
      241 ctgtcagcct accgcccgag atggttagat ctccctctcc agtgccgaga gtgctgcctc
      301 gaccgaccgt accgcggaga tgacgctgtg aaccagccgg ttccgctctc gcgcgagccg
      361 cgtgatcagg aattgtcgct ggtcacttgc ccgctccatg gctgccagct tggcgacggg
      421 ctgcagcccc gggaggggtt gcgtcggcac cgccaggtcc tggtcgaact gtccgctgcg
      481 agcatagttg acggggatga gtgcccgacc cagctccatc aggcagcggt tcaccacgcg
      541 ggccgccttg tcgctcaccc ggccctgctg cagcgccttg atccggccct gaagcctctt
      601 cactgccgaa cccagtgccg ccagttgctc ggccgcgggg gagagatcga atccgtcccc
      661 ggccttcgcc tgcaggtcat ccaccacagc cttcaactcg tcgaccacgg ccaggtagtc
      721 cagcggcagg acggtcgatg cgcacaaacg gtaggtcagc agcgtgtaga tgatggcatc
      781 ccgccgcaag aattcggggt cgatcttatc cagggtgtcc tcaggggtgt gccaccacga
      841 gccgaggccg ccgctcttcc cgtggccgcc gctgatctgg cgaaacaggg cggccagcac
      901 gggatcggct gcttcgggcg gctgctccga gagcgtcatc aacagcgagg gaatgccgtg
      961 cccccagaag gactggtccc ccgagcgccc gaagcggcga ggcgagagct gctggccggc
     1021 gaccgcctgg atgacttcgc tggcgaaccc ccgcatctcg gccatgctgt tgccctcggt
     1081 gagcaccgtc gcccccttgc cgccgacgga atccacgttg acgtgagccg cacagttgtc
     1141 gtggagatct tgccacttgt gatcggcgta gcgcgcggag ccggcgtagc gcccgtggga
     1201 gtgtcccgac cagaaggcga cccgaaggcc ccgccggagc gaggaccggt gcgctgcaag
     1261 caggcggacg acctcgagct gggtcgcgtt ggcgctcccg ttgtccatgg ccccgtagta
     1321 ccaggagtcc acgtggccgc tcagcatgat gaaggtgtgg gcttcaggcc tgtcggtggt
     1381 cgggcggagg tcgccggtca agcacgggat gtccttccac ccctggaaga ccctggtcag
     1441 cagcttcacc cggacggtcc cagtgcgcat ggcctccctg atgcgggccc cgtccagtcc
     1501 cttgatcgaa atggacgggg tccggggcaa gaggtgggcg gtctcgggcg tcggcgtgcc
     1561 ccacaccggc gacatcagcc gctcgtgcaa acgctgcccg gcgacccaga tggaggcggc
     1621 cacaccggcc gcctccagca ccaatccccg gttgggggcc gcgatgccgt ccaccaggac
     1681 gatcttcccg cgtacgtctc gcccggccag ttcttcttcc gtgccccggc cgacgtaaac
     1741 cagctcgcct tccagaccct cctctggcgt ggacgcggca aacccgtggg ttgcgcagat
     1801 gatttcctcg gtccttccgt cggccgtggt gatatgcagg gaggctgact ccggccagga
     1861 taccagagct gggaagctgt accgctcgac ctccagaccg taccacttca gttgagcctc
     1921 cagccagtcg aaggcatggg cctcctccgg cgtgccggaa aggcgaaccc actgagcgat
     1981 ggtcgcggtc gattccatga gtcgcttggt ggacacggca tccacgaatg cttgctcctc
     2041 agcgggaaag tgagccatca tcgcacatcc tccctgcgcc tcgaggcagg tgcagcccga
     2101 tccgcttcat tacccgaatg aatccatctc gcccggacgg acccggactc cacgcctgtt
     2161 ggtgagccgg catcgtgtac ccacccgaaa gagtgggcgc aggcgcccgg cgtcgagtac
     2221 ccaaccggac gttgggtgcg acgggccggg tggccggcac atgtcaccag ttgaagcccc
     2281 atcgaaacgg aagaaggctt ttccccatgg ttgttagatg agaaacgatg tgctcggatg
     2341 gagaccgcgt cccctcgcca ggtgggaatc tactgcgatg agttctaccc tgtcaacatc
     2401 gagtgcctct caaccctccg gattcatctc tccgtgcgaa ccggatcatt ctgcctcacg
     2461 ggttgcggtc tggagggaag atctccaacc tcgccaggac cccgtagtga tccgacggcc
     2521 ataggtaggt cccatcctgc aattgctggg gcctgttcaa tacgacacgg ctggatagga
     2581 tctttcccgg aaactccgtt ccagggcata cgaagaggta gtctgcccgg cgcttcacca
     2641 tcgaggcagg agcgtcgatg cgctgccaga cggtcaggcc gggctcgaac gggttgactg
     2701 tccggaaggc gtcaaccaaa ccccctttct gagtgatggc ggcgatggcc acggaatcct
     2761 cgggcgcgtt gaagtccccc atcagcacgg aggggaaggg gccgcgccga ttccggacga
     2821 gctgcgccac ccgcccggtc tggcaagggt ctccgatggt atgcgtggag aacacgtcaa
     2881 gcccgcccca cggactctgc agcctggcat gcaggaggag acgcgagtcg gcgtagctcc
     2941 cacagcgagg gaggtcaagg gattcccagg ccgcgatggg aaaacgggtt acgatcgccg
     3001 ggccctccga gaagttcagc agggaggcga tgaggcggtt gacccgactg ctctcgaacg
     3061 gccgtgggtt ggccgccgca aacacatact ggaggccgag ctgggcagcc agccgtgccg
     3121 ccacatttcc cctttgccgg ctctccgacg cctcctgcag gccgatgata tcggggtcca
     3181 gcacccgtaa ctccctcgcc accatctcca gccgacgatc cagttgctgc ccatctccca
     3241 ggaggccgga gaaagggccc ccatgataca ggttcagcgt gacaaccttg agcggtctcg
     3301 ccggctctgt catggagccg ccctccgccc gagccgcagg caggacgcag agacagacag
     3361 aagcggcgac cacggcaagg actcgattca tgcgtatcct cggagggctg gagtcttcga
     3421 tccaatcgtc atgaatgatt tcgaactgcc agggagaccc agcgtcaggg agcggcgtcg
     3481 agcagaagag tcaggggccg ttctcccttc gggaggggga catctcgcca gaagcatctg
     3541 ggctgaggcg aaaacgcatt cccgagacgc cgcgggcggg aggtctggca ccccccgccc
     3601 gcgtgaacgg cttccgatct gacgcctact tccagttctt gaatatcgtc tcgatccggg
     3661 tcaccgcctc gtccacagcc tgctcgttgc tccacttctc cgtcacgact cgggagatgg
     3721 ccttgcccca gacgttctcg gtatacacct ggacataggc cgggtggtag ctgaagccga
     3781 aggggcgggt cgggtacttg gagtgctgct ccgtcgccag ggagacgtgc gggtccttcg
     3841 ggtcgtggaa gaacttgtcc gccgccacgt ccttgaacga ggggaaccac cggccgttcg
     3901 acgccttcac atagtcggtg aaatgccggg tctccaggac gtatttgatg aacttcttgg
     3961 ccagcgccgg ctccttggcg tccttcggga tgatgatctg cttcaccgcc acgagatagg
     4021 gatacccctt cccatcgatg ggcgaggtgg gctggaggat gctcccgatc ttgttgtagt
     4081 agtaatcttt gttggtgaag aaccaagacc ccgggatgga gatggagggg ttgggcgtca
     4141 tgaccaccgt ccggttgttg aagttcacgt tgttgtcggc gtctgtccag gtaagggcgt
     4201 ctggggggac atagccttcc ttgtagatgc tggtgaagaa gctgagggtc ttgatgatgg
     4261 cctcgcggtt cttggcctcg ccggccacca ccttcccgtc gggggtgatc acatcgccat
     4321 gaaatgccag caggtactgc tcgaagttgt agatcgtgtc ggtcgagcgg ctggaagagg
     4381 tcataccgat tccgaagagc tttccgt
//
LOCUS       DBNGZC010000473         5497 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_431860, whole genome shotgun sequence.
ACCESSION   DBNGZC010000473 DBNGZC010000000
VERSION     DBNGZC010000473.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_431860"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="AC16G0_08325"
     CDS             <1..216
                     /locus_tag="AC16G0_08325"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR5099658.1"
                     /translation="EGSFSIVQASYRSALTERADVILPAPVWAERSGHVTNLEGTVLP
                     LAPAVPMPRGVRDEAEVLQALREMAAR"
     gene            253..810
                     /locus_tag="AC16G0_08330"
     CDS             253..810
                     /locus_tag="AC16G0_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIR5099659.1"
                     /translation="MGKIRLAMQWLEACSGCEMSMLDIDERMVELLRHAELTSSPITD
                     LKRPPQEGVEVGILSGGIGNEEEVAEAVQMRERSKTLIALGDCAVTGGICTMRNFFSR
                     EEVLRRGYIETESTVEGTVPTHEDLAPLTAQVTGVGEVVPVDIYLPGCPPSADAIWYV
                     LSELLAGRQPKLEGDKTLKYGLDGA"
     gene            816..2240
                     /locus_tag="AC16G0_08335"
     CDS             816..2240
                     /locus_tag="AC16G0_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012582502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HIR5099660.1"
                     /translation="MGQKITISPVTRIEGHAKITIHLDDAGHVERTLFHVEQFRGFEK
                     FSEGRMYYEMAQITPRICGICPVSHHLAATKAADQIAGTRPTATAHKLRELMHMGQVI
                     QSHGMHFFELAGPDLILGFDADPKIRNVAGLIAAEPDLALKAVQLRAFGQEIVRTLGG
                     RRIHPLFGVPGGVSQTLRPADRDRLRPGVDVQLASAQAGLTIWREWAERHRETLAEYA
                     VFPSGYLGLVKDDGALELYDGRLRLLDAAGAPLVEFAPQKYLDHIGEHVEPFSYLKFP
                     FYKPVGWPGGVYRVGPLARLNVATHIRTPLAQAEMVRWKALGDGKPVEGTLQYHYARL
                     IELLYALERAAELLDDQDITGEAVSNPAGPITGEGVGCLEAPRGTLIHHYRTDAQGVI
                     TRVNLIVATGHNNWAMNHAVSLVARRYMDGLPPTEGILNRIEAAIRAHDPCLSCSTHA
                     LGQMPLRVEFLAPDGRLLHTLCRD"
     gene            2249..2746
                     /locus_tag="AC16G0_08340"
     CDS             2249..2746
                     /locus_tag="AC16G0_08340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HIR5099661.1"
                     /translation="MRRSLVVGFGNPYRRDDGVGRAVVDLLRRELGQPALDPLDDGFD
                     ELGREVDTVVAHQLVPEFAELLGGYTLAIFVDAHVGDGTEPLCAEPIAPIPHSSSVTH
                     HLHPGTLLDLAQRIHGRTPRGVLLSLRGHDFEFGDGLSAETASLVPPAVARIRALIEG
                     ASADL"
     gene            complement(2954..3775)
                     /locus_tag="AC16G0_08345"
     CDS             complement(2954..3775)
                     /locus_tag="AC16G0_08345"
                     /inference="COORDINATES: protein motif:HMM:NF014826.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR5099662.1"
                     /translation="MAPRATGRLGPRSRRVSHSCSPTGEGLRAYTADLHVHTVLSPCA
                     AVEMIPPLIVQQALMRGLDLLAITDHNASANAGAVQRAAAGTGLTVLPGMELQTSEEV
                     HLLCVFDTLEQVASWQEEVDARMPRADNAPEVFGEQFVVNETGELIRQETRLLAGSVR
                     LRLEEAVGEVRRRGGLAIPAHIDRPSYSLPTSLGFPPPDLLMDALEISAHTTPAAARQ
                     RFPWLSGHPLVQGGDVHHLKDFAGTTVLTLAAPTISEIRLALRNQDGRACRILPG"
     gene            complement(3748..4095)
                     /locus_tag="AC16G0_08350"
     CDS             complement(3748..4095)
                     /locus_tag="AC16G0_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099663.1"
                     /translation="MTLEKIMQELELKLLTETLDLGKRTPTSGYASDMLSRVMAHAPR
                     DGALWVTLQSHANIVAVGAVLGLSGIIITEGELPDEATIAKAVEMGVPLLATPRPTFH
                     VVGQLWRLGLREG"
     gene            complement(4150..5160)
                     /locus_tag="AC16G0_08355"
     CDS             complement(4150..5160)
                     /locus_tag="AC16G0_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5099664.1"
                     /translation="MNVEVFPVAPDLPSARTIREELTQVEALAYELHVKDVMTSPVVS
                     VGPTDTISRLSEMLRTHNITGIPVVSDEKLVGIISVKDLIAALEKGELAAPVSAYMTS
                     NLFTLHEADALVRALEIFSKTGVGRLPVLDQEGRLVGIVTKGDIAHGLLRTLQTPKDE
                     EEVRRYRASHLFEDILSDRTSLFLRYRVRHRDFAGGGMASANVRRALLRLGASLETAR
                     RCAIAVYEAEMNLVIHTTNGGILRVEIQPELIFIEALDEGPGIEDIDRAMQPGYTTTP
                     PDIRAMGFGAGFGLANIQRFVDKMWLKSQPGGGTRLEMWIYLQPEAERRRLETMLDRL
                     ST"
BASE COUNT          892 a         1824 c         1865 g          916 t
ORIGIN      
        1 gaggggagct tcagcatcgt gcaggcaagc taccgatcgg ccctcacgga gcgggccgac
       61 gtgatcctgc cggcgcccgt ctgggccgag cgctcgggcc acgtgaccaa cctggaaggg
      121 accgtcctgc ctctggcgcc cgccgtgccg atgccccggg gggtgcggga cgaggcggag
      181 gtactccagg cgctgcggga gatggcggcg cgctgattcg gcgcggtttt cccgggccaa
      241 gtcgagggat tcatgggaaa gatcagactc gccatgcaat ggctggaagc ctgctccggc
      301 tgtgagatgt ccatgctgga cattgacgag cggatggtgg agttgctccg tcacgcggag
      361 ttgacctcct cgccgatcac cgacctcaag cgcccgcccc aggaaggggt ggaggtgggg
      421 atcctctccg ggggcatcgg gaacgaggag gaggtggcgg aggcggtgca gatgcgggag
      481 cggtccaaga ccctcatcgc gctgggtgat tgcgcggtca ccggcggcat ctgcaccatg
      541 cggaatttct tcagccggga ggaggtcttg cgccgggggt acatcgagac cgagagcacc
      601 gtggagggga cggtccccac ccatgaggat ctggccccgc tgacggcgca ggtgaccggc
      661 gtgggggagg tggtccccgt ggacatctac ctgcccggct gcccgccctc cgcggacgcc
      721 atctggtacg tgctgagcga gcttttggcc ggacggcaac cgaagctgga aggcgacaag
      781 accttgaagt acggcctgga cggagcgtag gcaccatggg gcagaagatc accatctcac
      841 ccgtgacccg catcgagggg cacgccaaga tcaccatcca cctggacgat gcaggccacg
      901 tggagcgcac cctcttccat gtcgagcagt tccggggctt cgagaaattc agcgagggcc
      961 ggatgtacta cgagatggcc cagatcacgc cccgcatctg cggcatctgc ccggtgagcc
     1021 atcacctggc cgcgaccaag gcggcggatc agatcgccgg cacccgtccg accgccaccg
     1081 cgcacaagct ccgggagctt atgcacatgg gacaggtcat ccagtcccac ggcatgcact
     1141 tcttcgagct ggcagggccc gacctgatcc tggggttcga cgccgatcca aagatccgga
     1201 acgtcgccgg gctcatcgcc gccgagccag acctggccct caaggcagtc cagctccgcg
     1261 ccttcggtca ggagatcgtc cgcaccctgg gcgggcgtcg catccacccc ctcttcggcg
     1321 tgccgggcgg cgtgagccag accctgcggc ccgcggaccg cgatcggctc cgaccgggag
     1381 tggacgtgca gcttgcgtcc gcgcaggcag gtctgacgat ctggcgggag tgggcggagc
     1441 gccaccggga aaccctcgcc gagtacgccg tgttcccctc cggctacctg ggcctggtca
     1501 aggacgacgg agccctggag ctttacgacg gacgcctccg gctgctggat gccgcgggcg
     1561 cgccgctcgt ggagtttgcg cctcagaagt atctcgatca catcggcgag catgtcgagc
     1621 ctttcagcta cctcaagttc ccgttctaca agccggtggg ctggcccggc ggcgtctatc
     1681 gcgtggggcc gctggcccgg ctgaacgtgg cgacgcacat ccggaccccg ctggcccagg
     1741 cggagatggt gcgctggaag gccctgggcg acgggaagcc ggtggagggc acgctccagt
     1801 accactacgc gcggctgatc gagctgctct acgccctgga gcgtgcggcg gagctgctgg
     1861 acgatcagga catcacggga gaggccgttt ccaatccggc cgggcctatc acgggggagg
     1921 gggtcggctg tctggaggcg ccccggggaa cgctcatcca ccactaccgg acggatgcgc
     1981 agggggtcat cacgcgggtg aacctgatcg tcgccaccgg gcacaacaac tgggccatga
     2041 accacgccgt cagcctggtg gcccggcggt acatggatgg cctgcctccg acagagggga
     2101 tcctgaaccg gatcgaggcc gccatccggg cccacgatcc ctgcctgtcc tgctccactc
     2161 acgccctggg ccagatgccc ctccgggtcg agttccttgc gccggacggg cggctgctcc
     2221 ataccctctg ccgggactag atctggcaat gaggcgcagc ctggtggtgg ggtttggcaa
     2281 tccctaccgg cgggatgacg gcgtggggcg cgcggtggtc gacttgctcc ggcgagagct
     2341 gggccagccg gccctcgacc ctctggatga cggctttgac gagctgggac gggaggtgga
     2401 cacggttgtc gcccaccagc tcgtccccga gttcgcggag cttctgggcg gctacaccct
     2461 ggcgatcttc gtggatgccc acgtgggcga tgggaccgaa ccactctgcg cggagcccat
     2521 cgcgccgatc ccccactcga gttccgtcac ccaccatctg caccccggga ccctcctcga
     2581 cctggcccag cgcatccacg gtcggacccc ccggggcgtg ctgctctcgc tcagaggtca
     2641 cgacttcgaa ttcggcgatg gcctctcggc cgagacggcg agtctcgtcc cgccggccgt
     2701 ggcccgtatc cgcgcgctga tcgagggcgc gagcgctgac ctctgatccc gaagaaatcc
     2761 gaaggtctca ccgccgagtc cgcgaagatc gcaaagaaga tacctgggga tctcactgga
     2821 gccgacggca gcccttcccc cggacgcttg aataatatcc gcccgtatcc gggggctgaa
     2881 tccttccgcg ctgcccacct ttgcgccctt tgcgcacttt gcggtgaggc tttggatgga
     2941 ggggggcggg ggatcagccg gggaggattc tacaggcgcg gccgtcctgg ttccgcaaag
     3001 ccagccggat ctcgctgatg gtcggggcgg ccagggtgag cacggtggtg ccggcgaagt
     3061 ccttcaggtg atggacgtcg ccgccctgga ccaaaggatg gccggacaac cagggaaatc
     3121 gctggcgggc ggccgcgggc gtggtgtggg ccgagatctc gagggcatcc atcaggagat
     3181 ccgggggagg aaagccgagg ctcgtgggca gactgtagct gggtcggtcg atatgggccg
     3241 ggatggcgag ccctccgcgg cgacgaacct cgccgaccgc ctcctccagg cgcagtcgga
     3301 ccgatccggc caagagccgc gtctcttgac gaatcagctc tcccgtttcg ttcaccacga
     3361 attgctcccc gaagacttca ggggcgttgt ccgcacgcgg cattcgggcg tccacctcct
     3421 cctgccagga cgccacttgc tccagcgtgt caaacacgca gaggaggtgc acctcctcgg
     3481 acgtctggag ctccatcccc ggcaagaccg tgagtccggt ccctgcggca gcccgttgca
     3541 ccgcaccggc gttggcgctg gcgttatggt cggtgatcgc gagaaggtcc aggcctcgca
     3601 tcagggcctg ttggactatc aggggaggga tcatctccac tgcggcacag ggggacagga
     3661 cagtgtgcac gtgcagatcg gccgtatacg cccggagccc ttcccccgtc gggctgcagg
     3721 agtgggacac gcgccgactg cgcggaccta gccttcccgt agcccgaggc gccacagctg
     3781 ccccactacg tgaaaggtcg gccgcggggt cgccaggagc ggcacaccca tctcgacggc
     3841 cttggcgatc gtggcctcgt cgggcagctc gccttccgtg atgatgatgc cgctcaaccc
     3901 cagcacagcg cccacggcca cgatattggc gtgggactgc agggtcaccc agagcgcccc
     3961 gtcccgaggg gcatgggcca tgacgcggct cagcatgtcc gaggcataac ctgacgtcgg
     4021 cgtccgcttt ccgagatcga gcgtctcggt caggagtttc agttcgagtt cctgcatgat
     4081 cttttcgagc gtcatggggc ctcctcgggc gagcgcgtcc gctcggcggt ccatccgtag
     4141 ccggccgggc tacgtgctga gccggtccag catggtctcg agcctccggc gctccgcctc
     4201 gggctggagg tagatccaca tttcgaggcg agttcccccg cccggctgtg acttgagcca
     4261 catcttgtcc acgaatcgct gaatattggc caacccgaat ccggcgccga agcccatggc
     4321 ccggatgtcc ggtggcgtcg tggtgtaacc gggctgcatg gcgcggtcga tgtcctcgat
     4381 cccggggccc tcatccaggg cctcgataaa tatcagctcg ggttggatct ccacccggag
     4441 gatcccgcca ttggttgtgt ggatcaccag gttcatctcg gcttcgtaca cggcgatggc
     4501 gcaacggcgg gcggtttcca ggctggcccc caaccggagg agcgcccgcc ggacattggc
     4561 ggacgccatc ccgccgcctg cgaagtctcg gtggcggacc cggtagcgca ggaacaggct
     4621 ggtccggtcg gacaggatgt cctcgaacag atggctggcc cggtagcgac ggacctcctc
     4681 ctcgtctttc ggagtctgga gcgtccgcag gaggccgtgg gcgatgtccc ctttggtgac
     4741 gatcccaacc aggcgaccct cctgatccaa gaccggcagg cgacctacgc cggtcttgga
     4801 aaatatctcc agggccctga ccaaggcatc ggcctcatgc aacgtgaaca ggttggaggt
     4861 catgtaggcg ctgaccggcg cagccagctc gcccttctcc aaggcggcga tcagatcctt
     4921 cacgctgatg atcccgacga gcttctcatc cgagaccacc ggaatgccgg tgatgttgtg
     4981 tgtgcggagc atctccgaca ggcggctgat ggtgtcggtg ggcccgacgc tcacgaccgg
     5041 actggtcatg acgtccttga cgtgtagctc gtacgccagg gcttcgacct gggtcagctc
     5101 ttctcggatg gtcctggcag agggaaggtc cggggccaca ggaaacacct ccacattcat
     5161 ctcgcgctat tgccggcggg agcataccgg aatttccccc cctagccaat cccgttcttg
     5221 ttctcgctcg tccccgcttg agcaaggata ttccttcact tcacctcgat ggggcttccc
     5281 atgccggatg cgggcataca ggcgacggcc gtgccggtgc ggccggacct agcgcttgag
     5341 gcaactggga ctcaccgcct tgatgcaggc ccacgggcgg gagcgggcga cgtccccgat
     5401 ctcgagccag atgaccacgg tcatcacccg ctgaggaccg acgcctgctt ccgccgcatg
     5461 cggtccaagc tctgggcatc ggggggcggc gcagcag
//
LOCUS       DBNGZC010000474         2937 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_626656, whole genome shotgun sequence.
ACCESSION   DBNGZC010000474 DBNGZC010000000
VERSION     DBNGZC010000474.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_626656"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /gene="pilQ"
                     /locus_tag="AC16G0_08360"
     CDS             <1..1013
                     /gene="pilQ"
                     /locus_tag="AC16G0_08360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HIR5099665.1"
                     /translation="QRAMEPLETEVIAVNHAKAADVVKNLDRLKTTGRPDTSIAVDER
                     TNKLIIKETRGTLDKMTQLLKQLDLPTPQVLIEARLVEAQRNFAQSLGVEWGFAANVN
                     MANLQAPPVSVFGSPTGTALVPTPAPGGGAIPLAVSFPALISGLETIPGVGFVANNLF
                     SNKLALQARLSAAENEGKLKTLSAPRVATLDNQEAEIKQGTQVPYTTIDSSGRTVVAF
                     QDAFIRLKVTPHITNDRRVSMKVEAERTDPGTQINFAGGFAFPLNTRKATTNVLVTNG
                     GTIVIGGLLQTTDNESESGIPWLNKVPVLSFLFKTDTISKTRIELLIFLTPTILEEPR
                     LS"
     gene            1196..2386
                     /gene="aroC"
                     /locus_tag="AC16G0_08365"
     CDS             1196..2386
                     /gene="aroC"
                     /locus_tag="AC16G0_08365"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR5099666.1"
                     /translation="MLRYLTAGESHGPALVAILEGMPSNVPVNPRDINADLVRRMGGY
                     GRGGRMKIERDEVQILGGIRRGITLGSPIALLIQNRDWENWKRTMAIEAEGVEGEAER
                     PPMTRPRPGHADLTGGLKYDHQDLRNVLERASARETAVRVAVGGACKCLLRQFGIQVV
                     SHVVEMGGIRAITEGMTAAEIAAKAEASPLRCADAAATAAIVAKVDEARSRGTSLGGV
                     FEVLVTDPPLGLGSYVHWDQRLDGRLAQALMSIQAIKGVEIGLGFETSRRFGSEVLDE
                     IFYQAPGQGSCQAEGFHRQTNFSGGLEGGMTTGQPLVLRAAMKPLSTQYTPLTSVDVV
                     TKEQSKAGVERSDITAVPAAGVVGEAVVAFEIARALCEKFGGDSLAEMQRNHAAYLAY
                     LRAV"
     gene            complement(2523..>2937)
                     /locus_tag="AC16G0_08370"
     CDS             complement(2523..>2937)
                     /locus_tag="AC16G0_08370"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR5099667.1"
                     /translation="QFVTSAMRLLLRIPLVDGLLIEVVEPGSPAEGAGLRGGQLEIGI
                     AGRSLLLGGDIITGVNGTPLTSTEKLVEVIQGLDVGATIHLTVFRDGEHRDVDYLLPE
                     RPILPMDLPGRRSITPAEGRRGESPAGHRLPPALR"
BASE COUNT          539 a          918 c         1009 g          471 t
ORIGIN      
        1 tccagagggc gatggagccg ctggagaccg aggtcatcgc cgtcaaccac gccaaggccg
       61 cggacgtcgt caagaacctg gaccgcctca agaccacggg ccgtcccgac accagcattg
      121 cggtggacga gcgaacgaac aagctgatca tcaaggagac ccggggcacg ctcgacaaga
      181 tgacgcagct cctgaagcaa ctggatctgc ccacgcccca ggtgttgatc gaggctcggc
      241 tggtggaggc gcaacgaaac ttcgcccaga gcctgggcgt cgaatgggga tttgcggcca
      301 acgtgaacat ggccaatctg caggccccac cggtgagcgt gttcggttca cccacgggga
      361 ccgccctggt cccgactccg gcccctggtg gcggcgccat tccattggcg gtctcgttcc
      421 ccgccttgat ttctggttta gagactatcc ctggcgttgg cttcgtggcc aacaatctct
      481 ttagcaacaa gttggccctg caggcccggt tatcggcggc ggagaatgag gggaagttga
      541 aaaccctctc tgcgcccagg gtggccaccc tggacaacca ggaggccgag atcaagcaag
      601 ggacccaggt tccctacacg accatagata gctcgggccg gacggtggtg gccttccagg
      661 atgcgttcat ccggctgaag gtgacccccc acatcaccaa cgatcgccgg gtgtccatga
      721 aggtcgaagc cgaacggacc gacccgggga ctcagatcaa cttcgccggc ggattcgcct
      781 tcccgctcaa cacgcggaag gccacgacca acgttctggt caccaacggc ggcaccatcg
      841 tgatcggcgg cctgcttcag actaccgaca atgagtctga atccgggatc ccttggctga
      901 ataaggttcc cgtcctgagt ttcctgttca agacggacac catctcgaaa acccggatcg
      961 agctgctgat cttcctcacc cccacgatcc tggaggagcc gcgattgagc tgacgccggc
     1021 atagtccgcg gcaccaaggc aaggagaaac gcacccccaa ccgggtgcgt tttctctttg
     1081 gtggagcggg gtggcagagt atcatgcgtc gccgtcacct gcgccccatg ccccggcgat
     1141 tccggacaga tgggcaggtg ggcgggccca catctcaggg gagaaaggtg gtgatatgct
     1201 caggtacttg acggccgggg aatcccacgg gccggcactg gtggctatcc ttgagggcat
     1261 gccctccaac gttccggtga acccgaggga tatcaacgcc gacctcgtcc gccgtatggg
     1321 gggctacggg cggggcggcc ggatgaagat cgagcgggat gaggtgcaga tcctgggagg
     1381 tatccggcgg gggataactt tgggcagccc catcgccctc ttgatccaaa accgcgactg
     1441 ggagaactgg aagcggacca tggccatcga ggccgagggc gttgagggcg aggcggagcg
     1501 tccgccgatg acgcgacccc gaccaggcca cgcggacctc acgggggggc tcaagtacga
     1561 ccaccaggat ctccgcaacg tcctggagag agcgtcggcg cgggagacgg ccgtgcgtgt
     1621 ggcggtggga ggcgcctgca aatgcctcct gcggcagttt gggatccagg tcgtgagcca
     1681 cgtcgtggag atgggaggca tccgagcgat cacggagggc atgacggcgg cggagattgc
     1741 ggccaaggca gaggcctcgc cgctgcgctg cgccgatgcc gcggccacgg cagccatcgt
     1801 ggccaaggtg gacgaggcgc gctcccgcgg cacctccctg gggggggtct tcgaggtgct
     1861 cgtgacggat ccacccctgg gcctcggcag ctatgtccac tgggatcagc gcctggacgg
     1921 ccgcctcgcc caggcgctca tgagcattca ggcgatcaaa ggggtggaga tcggccttgg
     1981 cttcgagacg tcacgtcgtt tcggctccga ggtcctcgac gagatcttct accaagctcc
     2041 gggtcagggt tcgtgccagg ccgagggctt tcaccggcag acgaacttca gcggcgggct
     2101 ggaggggggg atgaccaccg gtcagcccct cgttctccgc gccgccatga agcccctctc
     2161 cacccagtac accccactca cctcggtgga cgtggtgacc aaggagcaga gcaaggccgg
     2221 ggtggagcgc tctgacatca cggcggttcc ggccgcaggg gtggtcggcg aggcggtggt
     2281 ggccttcgag atcgcccggg ccttgtgcga gaagtttggg ggggacagtc tggcggagat
     2341 gcagcggaac cacgcggcct acttggccta cctcagggcc gtctgaggcc gggacatcac
     2401 ggcatccccg ccgtgcaaca gcgtggaaca gcaagggttc gtggaggggg aattcgacgc
     2461 ccactggttc aggcagcctc gagcggtcct gggtgtttct gaagggcgaa cgaccggggg
     2521 cgttaccgca gcgccggagg gaggcggtga cctgcggggg attctccgcg cctgccctcg
     2581 gcgggtgtga tggagcgtcg accggggaga tccatcggga ggatgggccg ctcggggagg
     2641 aggtaatcaa cgtcccgatg ctccccatcc cggaagacgg tcagatggat ggtggcgccg
     2701 acatccaaac cctgtatgac ctcgacgagc ttttcggtcg acgtcaacgg tgtgccgttc
     2761 acgccggtga tgatgtcccc gccgagaagc aggctcctcc cggcgatgcc gatctcaagc
     2821 tgtccgccgc gaagccccgc cccctcggcc gggcttcccg gctcgaccac ctcgatcagg
     2881 aggccgtcca ccagagggat gcggaggagg aggcgcatgg cgctggtcac gaactgc
//
LOCUS       DBNGZC010000475         2106 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2095607, whole genome shotgun sequence.
ACCESSION   DBNGZC010000475 DBNGZC010000000
VERSION     DBNGZC010000475.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2095607"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="AC16G0_08375"
     CDS             <1..197
                     /locus_tag="AC16G0_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099668.1"
                     /translation="VENKLEVTDIGPGLAEPIGLAVDVVEDLGVCEIYPSRSRSSYNP
                     YIRSESPTASRFLCLRIAVS"
     gene            194..1264
                     /locus_tag="AC16G0_08380"
     CDS             194..1264
                     /locus_tag="AC16G0_08380"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HIR5099669.1"
                     /translation="MSTPLALDARARAEIEERRKQVSDRRIFSRLCALLWLDDGMSQE
                     RVAQLLGITPRQVRKWVRTYRTQGLDGLATLHYQGDPGQLRRSQIERLKAEIQTGRFH
                     NARQIADWIEQTFQVRYSESGIKALLRRIGASYHKVSGFFGKADRKKQKAFVRKYRRH
                     RRQAGPTTRRYFIDACHPVWGLGLLYSCWLLVGQRFYVGVGDGRKRLNILGAYCPDDH
                     EYLDLCLTRGGINGEQFVNLLRLLRAAHPETEKFILYLDNAAYYKKALVRQWLKRHPE
                     FRLVFLPAYSPNLNLIERLWKFLRKEALSCWHKTFEEMQAAVSRVLDHLHEYREELDT
                     LMREEFQILRDEDLPVTSAVAA"
     gene            complement(1463..2050)
                     /locus_tag="AC16G0_08385"
                     /pseudo
     CDS             complement(1463..2050)
                     /locus_tag="AC16G0_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013793.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
BASE COUNT          381 a          677 c          667 g          381 t
ORIGIN      
        1 gtgtcgaaaa caagcttgag gtcaccgaca taggccctgg gctggccgaa ccgatcggtc
       61 ttgccgtgga tgtggttgag gatctcggcg tttgtgaaat atatccaagc cgttcgagaa
      121 gttcctacaa tccatatatt cggagcgagt cccctactgc atcccgattc ctgtgcttgc
      181 ggattgccgt ctcatgagta cgcccctcgc ccttgacgcc cgagcccgag ccgagatcga
      241 ggaacgccgg aagcaggtct cggaccgacg gatcttctcc cgcctttgcg ccctgctctg
      301 gctcgacgac gggatgtctc aggagcgggt cgcccaactc ctcgggatca ctccacgtca
      361 ggtccgcaag tgggtccgca cctaccgcac ccaagggctc gatgggctcg ccacgctcca
      421 ctaccagggc gaccccggtc aactccgccg ctcccagatc gaacggctca aggccgagat
      481 tcagaccggg cgcttccaca acgcacgcca gatcgccgac tggatcgagc agacgttcca
      541 ggtccgctac tccgagagcg ggatcaaggc gctgctgcgt cgcatcgggg ccagctacca
      601 caaggtcagc ggcttcttcg ggaaggccga ccggaagaag cagaaggcgt tcgtccgcaa
      661 gtatcggcga catcgccgcc aggcgggtcc gaccacccgg cggtatttca tcgatgcctg
      721 ccatcccgtc tggggactgg ggctgctcta ctcgtgctgg ctgctggtcg ggcagcgctt
      781 ctacgtcggt gtcggcgacg ggcgcaagcg gctgaacatc ctcggggcct actgccccga
      841 cgaccacgag tacctcgacc tgtgcctgac ccgaggcggc atcaatggcg agcaattcgt
      901 caacctgttg cgcctgctgc gtgcggctca tcccgagacc gaaaagttca tcctctacct
      961 ggacaacgcc gcctactaca agaaggcgct cgtccggcaa tggctgaagc gccacccgga
     1021 attccgcctg gtgttcctcc cggcctactc gcccaacctc aacttgatcg aacggctgtg
     1081 gaagttcctg cgcaaggagg cgttgtcatg ctggcacaag acgttcgagg agatgcaagc
     1141 ggcggtctct cgggtcctgg atcacctcca cgagtaccgg gaggaactgg acacgctgat
     1201 gcgggaggag ttccagatcc tccgagatga ggacctccct gtaacctcgg cggttgctgc
     1261 ttgaaggagt cctcacccct tcgctccgca gtcccttgag tttgcctgtc cttcggcccg
     1321 gtccgactgg cctgcggttc actacgcccg gcgagtgaca ggagaagaga tgaaggatgt
     1381 ggtcctccat ctcccaggag gatgtgtgtc ccattggagg aaccactcga ttggcgatgc
     1441 tatagctttg gtggacggcc aacctttggt cgtacatcgt gaccagatag cccgcgaggt
     1501 ggagtccagg gttcggaccg gcttgcactg cccccacggc cgaccgcacc ggaccgagcc
     1561 cttgggcacc gtagtcctcg gcctggagcg ggaccacgag gtaatccgat gccacgagcg
     1621 aggcgcgggc gcacaaatag aggttcgggg ggttgtccac cagggtcagg tcgaagctgt
     1681 tccgaatcgg ggcgaggaag tcgcggagtc cggtttccag gggcccccat tcccgcgccg
     1741 gtgtggtgtt gaccacggcc aggtggggcg agccggggat gatccagacc ccgtcaaccc
     1801 cggtcgggcg gacgagcgcg gccggtgagg gcgtggcgtc ctcgtcgtag aggccggcga
     1861 tgctctcgcc ggccgggacg gagccgatcg ccgccggtcc gtaaaacccc tgagtcagac
     1921 tcgcctgggg gtcgttgtcg agcagcaaga cccgcttgcc catccgggcg aacgtgccgg
     1981 ccaagtgatg gcaagtgctc gtcttgccga ctccgccttt caggttcaag aacgagatca
     2041 catggcccat aagaccctcc ctggcggcgg gcgctcaacc gttgagcgag atctacagaa
     2101 gggtta
//
LOCUS       DBNGZC010000476         2790 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1801663, whole genome shotgun sequence.
ACCESSION   DBNGZC010000476 DBNGZC010000000
VERSION     DBNGZC010000476.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1801663"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="AC16G0_08390"
     CDS             complement(<1..399)
                     /locus_tag="AC16G0_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HIR5099670.1"
                     /translation="MNASRLSKSKYLAGLQCLKRLYLDIHARDLASEVDAGTQAVLDA
                     GTRVGELARDRYPGGVLVEGDYFKVADGLARTAAVLADPGVPAIYEGFIQCDDVLVRP
                     DILVRTRGNRWRLIEVKSTSRVKDEHLDDLA"
     gene            complement(417..998)
                     /locus_tag="AC16G0_08395"
     CDS             complement(417..998)
                     /locus_tag="AC16G0_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099671.1"
                     /translation="MDTKSNFLRQQVFALLRESTTSAEEDLKALSQCGAHIRNRMIVE
                     GIARDCDLTLARFLTNDALELYYDIKRSRHDMGFISKGWDDPGFRIGDLVEVGWWDGD
                     VLKANSQHIMRFCATNGITMTIQEAPLAISLQLDGVIYSEGFNRGTFLQTLDSLNACV
                     EKIHALIPGGQHGRHSPASILCRRMPEASGRTH"
     gene            complement(1128..2057)
                     /locus_tag="AC16G0_08400"
     CDS             complement(1128..2057)
                     /locus_tag="AC16G0_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008179308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIR5099672.1"
                     /translation="MEPSERFRGALLGLAVGDALGTTLEFKPPGTFEPITDLVGGGPF
                     RLKPGQWTDDTSMALCLADSLIESRGYDPADQLRRYCRWYREGYLSSTGRCFDIGNTT
                     REALERFEQTHESFCGSTDPRTAGNGSIMRLAPVALFYAQRPREAIERAGESSRTTHG
                     AREAVDACRYFAGLIIGALQGCSKDDLLSPYFSPVPGLWTETPLAPKIAAVAAGSFKA
                     KDPPAIQGSGYVVDSLEAALWAFHRSHSFKDGALLAVNLGDDADTTGAVYGQLAGAYY
                     GEEGIPEAWRVKLAYREVIEGYAEQLRLLALTA"
     gene            complement(2157..2561)
                     /locus_tag="AC16G0_08405"
     CDS             complement(2157..2561)
                     /locus_tag="AC16G0_08405"
                     /inference="COORDINATES: protein motif:HMM:NF017020.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HIR5099673.1"
                     /translation="MKPELAALATHRMAQARETLAEAEQLLASQALRGAVNRLYYAAF
                     YAARALLATRDMDAARHSGLITLFQQHFVKTGLVPTETGKVLARAFEKRQTSDYGDFA
                     IIDPADAQDLSRAVHAFVHACGELLQRLVAGA"
     gene            complement(2558..>2790)
                     /locus_tag="AC16G0_08410"
     CDS             complement(2558..>2790)
                     /locus_tag="AC16G0_08410"
                     /inference="COORDINATES: protein motif:HMM:NF014021.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR5099674.1"
                     /translation="SHVRGEAAPDSDLDVLVAVEEATPAFENAILDLAFQVNLAHAVY
                     ISPRVIARQVFEDPVWGSTPFIRALQAEGAPL"
BASE COUNT          525 a          876 c          906 g          483 t
ORIGIN      
        1 ggccaggtcg tccaggtgtt cgtccttcac ccgactggtg gacttcacct cgatcagccg
       61 ccaccggttg ccgcgggttc tgacgaggat gtccggccgc acgaggacat catcgcactg
      121 gatgaacccc tcgtagatcg cgggtacacc cggatcggca agcacggccg ctgtccgggc
      181 gagcccatcg gccaccttga agtagtcgcc ctccacgaga acgccgcccg gataccggtc
      241 ccgggcgagt tcccccaccc gagtgccagc atcgagcacc gcctgggtac ccgcgtcgac
      301 ctcgctcgcc aggtctcggg cgtggatgtc cagatacagg cgcttcaggc actggaggcc
      361 ggcgaggtac ttggacttgg aaaggcgtga agcgttcatc ttttcacgca gaatgcctaa
      421 tgggtccggc cagatgcttc cggcatcctt ctgcaaagga tgctggcagg agaatgacgg
      481 ccatgctgac ctccgggaat cagggcgtga atcttctcca cgcaggcatt caggctatcg
      541 agggtctgga gaaatgtccc ccgattgaac ccctcgctgt agatcacgcc atccaattgg
      601 agtgagatgg cgagcggcgc ttcctggatc gtcatcgtga tgccattggt cgcacaaaag
      661 cgcatgatgt gctgcgagtt ggctttcaag acgtccccgt cccaccaacc gacctccacc
      721 agatcgccga tccggaaccc gggatcgtcc cagcctttgg aaatgaagcc catgtcgtgg
      781 cggctgcgct tgatgtcgta gtacaactcc agggcgtcgt tcgtaaggaa tcgtgcgagg
      841 gtcaagtcgc aatcccgggc gatgccctca acgatcattc ggttccggat gtgggccccg
      901 cattgcgaaa gagctttcaa atcctcttcg gcggaggtcg tgctctcgcg caggagcgca
      961 aagacttgct ggcgtagaaa gttgctctta gtatccatag aatcctcctg tggccggctt
     1021 gaatctggac cggcgcacaa ggaggatacc gccgggctgt gacatctagg gtcacagaaa
     1081 aatttgggac cgccggaatc gacggaacgc atcccaacgg gggcaaatca cgcagtgagt
     1141 gcgagcaaac ggagttgctc cgcgtatcct tcaatcacct cgcgatacgc tagcttgacg
     1201 cgccaggcct ccggaatccc ctcctctccg tagtaggcgc cagcaagctg cccgtagacg
     1261 gcgccggtgg tgtcggcgtc gtcgccgagg ttcacggcga ggagggcccc gtctttgaat
     1321 gagtggctcc gatgaaatgc ccaaagggcg gcctccaggc tgtccaccac gtagccggag
     1381 ccttggattg ctggtggatc cttcgccttg aaggacccgg ctgcgacggc agcgatcttc
     1441 ggagccaggg gggtctcggt ccacagaccg gggaccggag agaaatatgg ggacagcaag
     1501 tcatccttcg agcagccctg gagggcaccg atgatcaggc cggcaaagta ccggcaggca
     1561 tccaccgctt ctcgggcgcc gtgggtggtc cgggaactct ccccggctct ctcgatggcc
     1621 tcccgcgggc gctgcgcgta gaacagggcc acgggggcga ggcgcatgat cgagccgttc
     1681 cccgccgtcc gcggatcggt ggagccacag aatgactcgt gggtctgctc gaaccgttcc
     1741 agggcctcgc gggtggtgtt cccgatgtcg aagcagcgcc ccgtgctgct gagatagccc
     1801 tcgcgatacc agcggcagta ccggcgaagc tggtcggccg ggtcgtatcc ccggctttcg
     1861 atcaggctat ccgccaggca cagggccatg gaggtgtcat cggtccactg gcccggcttc
     1921 agcctgaacg gacccccacc caccaggtcg gtgatcggtt cgaaagttcc tggcggcttg
     1981 aactccagtg tggtcccgag cgcatcgccg acggccagcc ccaacagagc gcctcggaaa
     2041 cgctcactgg gttccatagc gctctcggat cacgggaagg gcaccaaggg gatggagcag
     2101 aagggaatca gcacgaccgg tgatagggac aggggatcta gcgatcgttg gaccgatcat
     2161 gccccggcaa ccagccgctg cagcaactca ccgcaggcat gcacgaacgc gtggacggcc
     2221 cgggaaaggt cttgagcgtc tgcggggtcg atgatagcga agtcgccgta atcggaggtc
     2281 tggcgcttct cgaaggcacg ggccaagacc ttgccggtct cggtcggcac gaggccggtc
     2341 ttcacgaagt gctgctggaa gagggtgatg agcccgctat gtcgagcggc atccatatcc
     2401 cgagtggcca gcagggcgcg cgccgcatag aaggcggcgt aatacagccg gttcaccgcc
     2461 ccacgaagcg cctggcttgc caggagctgc tccgcctcgg ccagcgtctc tcgggcctgc
     2521 gccatgcgat gcgtcgccag cgcggcgagc tcgggcttca caggggggcc ccctcggcct
     2581 ggagggcgcg aatgaaggga gtgctccccc acaccggatc ctcgaacacc tgtcgagcga
     2641 tcacgcgggg ggagatatac actgcatggg cgaggttcac ctggaacgcc aggtccagaa
     2701 tcgcattctc gaaggcgggg gtcgcctcct ctacggcgac gaggacatcg agatccgaat
     2761 cgggcgccgc ctcgcctcgg acgtgggacc
//
LOCUS       DBNGZC010000477         1833 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2484837, whole genome shotgun sequence.
ACCESSION   DBNGZC010000477 DBNGZC010000000
VERSION     DBNGZC010000477.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2484837"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..804
                     /locus_tag="AC16G0_08415"
     CDS             106..804
                     /locus_tag="AC16G0_08415"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008675 -
                     2-dehydro-3-deoxy-phosphogluconate aldolase activity
                     [Evidence IEA];
                     GO_function: GO:0008700 - (R,S)-4-hydroxy-2-oxoglutarate
                     aldolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HIR5099675.1"
                     /translation="MSVNQVVERIVAGGVIAIIPFPGPSELLPAAEALREGGISAIEF
                     TSTSPGAIQAIEAARIRLRGSVLLGVGAVLSPEAARDAIRAGAEFLVAPNLNPEVIRA
                     GREAGVAVVPGAFTPTEIVQAWDLEASLVQVFPAGPLGPRYIKELHGSLPHIPLVPAG
                     GVSLENVGAFIYAGASAVRVGGELVSQDLLEQGASREITGRAQEFAEAIRRARRASGY
                     AVTPIKPIEGPDIR"
     gene            complement(1054..>1833)
                     /locus_tag="AC16G0_08420"
     CDS             complement(1054..>1833)
                     /locus_tag="AC16G0_08420"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5099676.1"
                     /translation="ARFMLAPAPGWRPAGWTGADAAARTRLQQSSRSYTPLEMIRTGR
                     FWLMYVMMSLVAFGGLMVTAQLKPIANSYGLDKSVVWLGITALGMALMLDRILNGVTR
                     PFWGWVSDHIGRYNTMALAFGAEALAILALLRLVHQPIWFVILTGFTFFAWGEIFSLF
                     PATIGDVFGPDYATTNYGIQYTAKGTASIFAGWGAAKLLEMSGSWIPVFWVAVVCDLT
                     AAILAYFWLKPMVVRALQRERHVQAGVTTGQPVTSMAGGTD"
BASE COUNT          336 a          584 c          616 g          297 t
ORIGIN      
        1 ggtccccaag tgggtgtaag ggatggcccc gcgcctgccg acctatttgc ttttcctcac
       61 cgcctggaaa gggtagcggg ctctgcggag agttagggat agcgcatgag cgtgaaccag
      121 gtagtagagc ggatcgtggc ggggggcgtc atcgcgatca ttccgtttcc gggtccgagc
      181 gagctgctgc cagcggcgga ggctctccgg gaaggcggaa tatccgccat cgagttcacc
      241 tcgacctcgc ccggcgccat ccaggcgatc gaagccgccc ggatcaggct gcgcggcagc
      301 gtcctcctcg gcgtcggggc ggtcctttca cccgaggcgg cgcgggatgc gatccgtgcc
      361 ggggccgagt ttctggtagc gcccaacctg aacccggagg tgatccgcgc cggccgagag
      421 gcgggggttg cggtcgtacc gggggccttc acgccgacgg agatcgtgca ggcctgggac
      481 ttggaggcca gcctggtaca ggtatttccc gcggggccgc tcggtccccg ctacatcaaa
      541 gagttgcacg gatcgctccc ccacatcccc ctggttcccg cgggaggcgt ctcgctggag
      601 aacgtgggcg cgttcattta tgcgggcgcg tctgcggtcc gggtgggcgg cgaactggtc
      661 tcgcaggacc tcctggaaca gggggcgtct cgtgagatca ccggtcgcgc gcaggagttc
      721 gcggaggcaa tccggcgagc gcgtcgggcg tcgggatacg ctgtgacccc gatcaaaccg
      781 atcgaaggac ccgacatccg ttagagaccc gatatccggc tgggagagcg acagccaggt
      841 acgacagggg aggggtcaga gacagcgccg acacccagcc aggtgggaga ctccaccgtc
      901 tcgacgtcag cagagaagtc cgggagtgcc gaaacgcccg atacggaagc gcatgtttca
      961 caacaaaaga ggccagggcg gtctttccgc cccggcctct tttgcagcga ttgccaccgc
     1021 cgaacggatg gactgtcttg gaggcgctgc gtgctagtcc gttcccccgg ccatggaggt
     1081 taccggctgt cctgtagtta cacccgcctg gacgtggcgt tctcgctgca gagccctcac
     1141 gaccatgggc ttgagccaga agtaggcgag gattgccgcc gtcaggtcgc acaccaccgc
     1201 gacccagaac acgggaatcc aggaccctga catctccaag agcttggcgg cgccccagcc
     1261 ggcgaagatg gacgccgtcc ccttggctgt gtattggatg ccataattgg tcgtggcata
     1321 gtcgggaccg aacacatcgc cgatcgttgc cgggaagagc gagaaaatct ccccccaggc
     1381 aaagaacgtg aagcccgtca ggatcacgaa ccagatgggc tggtggacca gtcgcaggag
     1441 ggcgagaatc gccaaagcct ccgcaccgaa ggcgagggcc atggtattgt agcgcccgat
     1501 gtggtcagag acccatcccc agaacggccg cgtaacccca ttcaggatcc ggtcgagcat
     1561 cagggccatg cccagcgccg tgatccccag ccacaccacg gatttgtcca agccgtatga
     1621 gttggcgatc ggcttgagct gggccgtcac catgagcccc ccgaaggcga ccagactcat
     1681 catcacgtac atcagccaga agcggccggt ccggatcatc tccagtggcg tgtagctcct
     1741 gctcgactgc tggagccgag ttctggccgc agcatccgct cccgtccatc cggcgggccg
     1801 ccatccgggt gccggcgcga gcatgaaccg ggc
//
LOCUS       DBNGZC010000478         4020 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1311442, whole genome shotgun sequence.
ACCESSION   DBNGZC010000478 DBNGZC010000000
VERSION     DBNGZC010000478.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1311442"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2564)
                     /locus_tag="AC16G0_08425"
     CDS             complement(<1..2564)
                     /locus_tag="AC16G0_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - L-glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HIR5099677.1"
                     /translation="MPSSTDQRLDRSILKSIRATRGAAGENLRWLREQMPPFFFISMR
                     DEEEALANLAINLNWLQRNRHLILADQEKELILARLDVPGSIYETLEQIQDREVSYGE
                     MSHSYAVVPGTDLPLEIQRYEFDRKTDAEIAQAGDVSIPARIRREVIAALHAHYPPMD
                     ARERERLFRLIWLNNERYVRVSPARRVAQLLWLFHEGRAHGGIFLDVNAEGYEEHQET
                     KVLFAVGNPPHREYLTQVMEVFNRLNLGVRRCYTLTLSTGVHPYFLGSFYVARRGGGT
                     IEPESELLWKLRRELYNTQILATETPTYRDFVVRRVLTGEEASLIDAFIGFCHTNLVH
                     NQPHRYTLEDVMRAFHSHPDIALRLVRLFEARFDPDLPDRDARYRDELEQTEREVAAY
                     NTGHKRLDEFRRSIFHTTVLFIRRTLKTNFFIPEKHALAFRLDPAYLDDLGPDFTADL
                     PPERPFRVTYFYGRHGLGYHIGFSDIARGGWRTIITQTRDDYVTVANTLFRENYVLAH
                     TQHLKNKDIYEGGSKLVVALRAPDARTKERLNQLLYKIQYDFINAFLDIFITQEGKAK
                     DPRVVDYYGEDEPIELGPDENMHDLMIETIAELSVKRGYVLGIGIMSSKVVGINHKHY
                     GVTSTGVVKFAEVAMRQQGIDIRRDPFSIKFTGGPNGDVAGNAMRLLLERCPCVAIRL
                     IVDGTGAVVDPKGLDGKELSRIVLQQDVEAFDPACLSPGGFLLYRNVRRTEGLRELYK
                     RVARTETGPEEAWVTLDEFHKEFNELLFTVPADLFIPAGGRPETVDRSNWGRFFGPDG
                     TPTARVIVEGANSFITPEARTLLQQKGIVVLRDASANKCGVISSSYEIIGNLLLTP"
     gene            complement(2737..3015)
                     /locus_tag="AC16G0_08430"
     CDS             complement(2737..3015)
                     /locus_tag="AC16G0_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI-like protein"
                     /protein_id="HIR5099678.1"
                     /translation="MYYMLLYDVVDNFIERRTVFREEHLKQVRDAHDRGELWLAGALV
                     DPVDGAALVFTTEDRSVAERFAQNDPYVKGGLVKQWRVRQWNVVVGGK"
     gene            complement(3019..3345)
                     /locus_tag="AC16G0_08435"
     CDS             complement(3019..3345)
                     /locus_tag="AC16G0_08435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIR5099679.1"
                     /translation="MIARVWRGWTSRGNADAYEHHLRTTVFPGIHRVRGYRGAQLFRR
                     QEDEEVEFIAITYFETMDAVRGFAGPDCGVAVISEVAAKLLSHYDQRAEHYTVVLGPE
                     ASPVKG"
     gene            complement(3360..>4020)
                     /locus_tag="AC16G0_08440"
     CDS             complement(3360..>4020)
                     /locus_tag="AC16G0_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR5099680.1"
                     /translation="LTAWYGLVELGALEAGDAVLVHSAAGGVGLNALAILAAYDARVV
                     ATVGRPAKRDFLVERWGLSPDQVIVRDRRTFGEQLDRALAALRLDGFDLALDAIAGPF
                     FQPAYRRLRPGGRHVVYGAADFMPRGRRPDYLRLGLKYLRRPRLDPLRMIAENRSLMA
                     FNLIWLWDQVHRLQLAADQVARLLGKPPLVGRRFAFTDAPAALRYLQSGESVGKVVLE
                     V"
BASE COUNT          696 a         1295 c         1328 g          701 t
ORIGIN      
        1 ttcggggtca ggagcaggtt gccgatgatc tcgtacgaag aagagatgac gccgcacttg
       61 ttggccgacg cgtcccgcag gacgacgatc cccttctgct gcaacagggt cctggcctcg
      121 ggggtgatga aggagttggc tccctccacg atcacgcgcg ccgtgggcgt gccgtccggg
      181 ccgaagaacc gcccccagtt cgaccgatcc acggtctcgg gccgtccgcc ggcggggatg
      241 aacaggtcgg cgggaaccgt gaaaagaagt tcgttgaatt ctttgtggaa ttcgtccagg
      301 gtgacccacg cctcctccgg accggtctcc gtccgcgcga cacgcttgta gagttcccgc
      361 agtccctctg tccggcggac gtttcgatac aggaggaagc caccggggga aaggcaggcc
      421 gggtcgaagg cctccacgtc ctgctggaga acgatgcggg acagctcctt tccgtccaga
      481 cccttcggat cgaccacggc gccggtgccg tccacgatga gtcgaatggc gacgcagggg
      541 cagcgctcca gcaggaggcg catggcgttc cccgcgacat ccccgtttgg gcctccggtg
      601 aacttgatgc tgaacgggtc gcggcggata tcgatcccct gctggcgcat ggcgacttcc
      661 gcaaatttca ccacgccggt ggaggtgacc ccgtagtgct tgtggttgat gccgacgacc
      721 ttgctggaca tgatgccgat gccaagcacg tagccgcgct tgacggacag ctccgcgatg
      781 gtctcgatca tcaggtcgtg catgttctcg tcagggccga gctcgatggg ttcgtcttcg
      841 ccataatagt ccacgacccg cggatctttg gccttcccct cctgcgtgat gaagatgtcc
      901 agaaaggcgt tgatgaaatc gtactggatc ttgtagagga gctggttcaa ccgctccttc
      961 gtccgggcgt ccggggcccg cagcgccacg accagcttgg agccgccctc gtaaatgtcc
     1021 ttgttcttga ggtgctgggt gtgagcgagg acgtagtttt cccggaacag cgtgttcgcc
     1081 acggtgacgt aatcgtcgcg cgtctgggtg atgatggtcc gccagccgcc ccgcgcgatg
     1141 tcggaaaatc cgatgtggta ccccagtccg tggcggccgt agaagtaggt gacccggaag
     1201 ggccgctccg gggggagatc ggccgtgaag tccggcccca ggtcatccag ataggcgggg
     1261 tcgaggcgga aggccagggc atgcttctcg ggaatgaaga agttggtctt gagcgtgcgc
     1321 cggatgaaga ggaccgtggt gtggaagatg gaacgacgga attcgtccag ccgcttgtgg
     1381 ccggtgttgt aggcggcgac ctcgcgttcg gtctgctcca gctcgtcgcg ataccgcgcg
     1441 tcccggtccg gcaggtccgg atcgaagcgg gcctcgaaga ggcggaccag ccgcagggcg
     1501 atgtcggggt gcgagtggaa ggcccgcatc acgtcctcga gcgtgtaccg gtgaggttgg
     1561 ttgtggacga ggttggtgtg gcagaagccg atgaaggcat cgatgaggga ggcctcttct
     1621 cccgtcagga cgcgccggac gacgaagtcc cggtacgtcg gggtctccgt ggcaaggatc
     1681 tgggtgttgt acagctcgcg tcgcaacttc cagagcagct cggactccgg ctcgatggtc
     1741 ccgccgcccc ggcgggcgac gtagaagctc ccgaggaagt aggggtgcac gcccgtgctg
     1801 agggtcagcg tatagcagcg ccgcacgcca aggttgaggc ggttgaacac ctccatgacc
     1861 tgggtgagat actcccggtg cggcgggttc ccgaccgcga agaggacctt ggtctcctgg
     1921 tgctcctcgt agccctcggc gttcacatcc aggaagatgc cgccgtgggc ccgaccctcg
     1981 tggaacagcc agagaagctg ggcgacccgg cgcgccggag agacccgcac gtaccgctca
     2041 ttgttgagcc agatcaggcg gaaaagcctc tcccgctcgc gggcatccat gggaggataa
     2101 tgcgcgtgga gggctgcaat cacctcgcgg cgaatgcggg ccgggatgga cacgtcgccc
     2161 gcctgggcga tctccgcgtc ggtcttccgg tcaaactcgt accgctgaat ctccagggga
     2221 aggtctgtgc ccgggaccac ggcatacgaa tgagacatct cgccgtagga cacttcccga
     2281 tcctggatct gctccaaggt ctcgtagatc gagcctggga cgtccagccg ggcgaggatg
     2341 agctccttct cctggtcggc gagaatcaga tgccggttgc gctggagcca gttcaggttg
     2401 atggcgaggt tcgccagggc ttcctcttca tcccgcatcg agatgaagaa gaagggcggc
     2461 atctgctcgc ggagccaccg gagattttcc ccggcagccc ccctggtggc gcggatggat
     2521 ttcaggatgc tgcgatccag acgctggtcc gtggaggaag gcatggctct ctcctacgcg
     2581 aggctgcctc gccccgctgt cccaggctgt gccccaggtc actgttcaac gcggttcgcc
     2641 cggcccacgc gagtgtgccc tgcgcttccc gggagcgtgc ctcacacgct cgtgggtgtc
     2701 aaggggaatc cgccgaccgc gccgggtgca gtaggctcac tttcccccca cgaccacatt
     2761 ccactgccgg accctccact gtttcacaag tccgcccttc acatacgggt cgttctgagc
     2821 gaaacgctcg gccacggacc ggtcctccgt cgtgaagacc agggccgcgc cgtccacagg
     2881 atccaccagg gcgcccgcca gccacagctc gccccgatcg tgggcatctc gcacctgctt
     2941 caggtgctcc tcgcggaaga cggtccggcg ctcgatgaag ttgtcgacca catcgtagag
     3001 cagcatgtag tacatgggtc accctttcac tggggacgcc tctggcccga gaactaccgt
     3061 gtagtgctcc gcccgctggt cgtagtgcga caggagcttt gccgccactt ccgatatcac
     3121 cgcgactccg caatcgggcc cggcgaaacc gcggaccgca tccatggtct cgaagtaggt
     3181 gatggcgata aattccacct cctcgtcctc ctgccgccga aagagctgag cgcccctgta
     3241 gccgcggacc cgatggatgc cgggaaacac cgtggtccgc aggtggtgct cataggcatc
     3301 ggcgttccca cgtgacgtcc agccgcgcca gaccctcgcg atcatccttt gccctcctct
     3361 tacacctcca gcaccacctt gccgacactc tcgccactct gcaggtaacg gagcgctgcg
     3421 ggcgcatcgg tgaaggcgaa gcgccgcccg acgaggggcg gtttgcccaa gagcctggcg
     3481 acctgatcgg ccgccagttg caggcggtgg acctgatccc agagccagat cagattgaaa
     3541 gccatcaggc tgcgattctc agcgatcata cgaagcgggt caagccgcgg gcgccgcagg
     3601 tacttgaggc ccaggcggag atagtccggc cgcctgcccc ggggcatgaa atcggccgcg
     3661 ccgtagacga cgtggcgccc cccgggccgc aggcgccggt acgcgggctg gaaaaatggg
     3721 ccggcaatcg catccaacgc caggtcaaac ccatccagcc gcaaggctgc cagcgcgcgg
     3781 tcgagctgtt ccccgaaggt ccggcggtcc cgcacaatca cctggtcggg cgagagaccc
     3841 cagcgctcga cgagaaagtc gcgcttggcc ggacgcccga ccgtggcgac cacccgcgcg
     3901 tcgtaggctg cgaggatcgc aagcgcgttg aggcccacgc cccctgccgc cgagtgcacg
     3961 agcacggcgt cgccggcctc cagtgcgccc agctcgacca gtccatacca ggcagtgagt
//
LOCUS       DBNGZC010000479         1839 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1410215, whole genome shotgun sequence.
ACCESSION   DBNGZC010000479 DBNGZC010000000
VERSION     DBNGZC010000479.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1410215"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..1202)
                     /locus_tag="AC16G0_08445"
     CDS             complement(51..1202)
                     /locus_tag="AC16G0_08445"
                     /inference="COORDINATES: protein motif:HMM:NF017197.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HIR5099681.1"
                     /translation="MTSRKGKRWTLAAAAVLFVILIAGGIVGFRVALSVLKGKVIEAL
                     GPGSDITELRVGWSALEVLGLRIKGSQGWPAPDALRAERVVIVPSLRSLFSDQVRIAS
                     ITVTKPYLSMLRTRDGRLRVMPSLLEKPAAMGQPAAGPPIRSVAVSRFDMEDGVVELF
                     DETVAQPPLKVRLEQVQASVRNVLVPALRGRSQFDLAAIVKGIRRDGRAKVTGWIEAA
                     SKDSSVKTELRGVDLVAFQPYLSKASETRIQRGGLDLDLASEVSKNRLRAPGKVTISD
                     LEFAPAHGAMDTFMGLPRAGVVSFLKNKDNKIALDFTIEGDVNNPKFTLNEAFTTRVA
                     SSMAENLGLNIKGVAEGVGGLGRKGAEAVGEAAKGVGGALQRLFGGQKK"
     gene            complement(1257..1667)
                     /locus_tag="AC16G0_08450"
     CDS             complement(1257..1667)
                     /locus_tag="AC16G0_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HIR5099682.1"
                     /translation="MRNRWLLKTEPGEYSFEGLMRERKTVWDGVTNPLALKHLRSIRK
                     GDELLIYHTGAIRAAVGLAKAASAPYPDPERQDPKIVVVDVVPVRKLRQAVTLEAIKS
                     DRRFKDFELVRLPRLSVMPVPDAAWDAILRMAGG"
BASE COUNT          323 a          642 c          516 g          358 t
ORIGIN      
        1 atggcgccac gcgctagcgc cagccaggag cggccttcgt gaggtcgggc ttacttcttc
       61 tgcccgccga agagccgttg cagggcgccc cccacgccct tggcggcctc gcccaccgcc
      121 tcggcgccct tccgtcccag gccgcccacg ccctcggcga cgcctttgat gttcaacccc
      181 aggttctccg ccatggagga cgccacgcgg gtcgtgaagg cctcgttgag ggtaaacttc
      241 gggttgttga cgtccccttc gatggtgaaa tccagggcga tcttgttgtc cttgtttttc
      301 aggaagctga ccacaccggc ccgtggcagt cccatgaacg tgtccatcgc cccgtgggcg
      361 ggcgcaaact ccagatccga aatggtcacc tttcccggcg cccggagccg gttcttgctg
      421 acctcggagg caagatccag gtccagacct cctcgctgga tccgggtctc cgacgccttg
      481 ctgaggtacg gctggaatgc caccaggtcc acgccgcgca gttcggtctt taccgatgag
      541 tctttgctgg ccgcctcgat ccaccccgtc accttggcgc gcccgtcccg ccggataccc
      601 ttcacgattg cggccaggtc gaattgactc ctccctctga gcgccgggac cagtacgttt
      661 cgcacggagg cctgcacctg ctcaagacgg accttgagcg ggggctgggc caccgtttcg
      721 tcgaaaagct ccaccacgcc gtcctccatg tcgaagcgcg agacggccac gctgcgtatc
      781 ggggggccgg ctgccggctg ccccatcgcc gccggtttct ccagcaagct gggcatgacc
      841 cgcaatctcc catcccgagt ccgaagcatc gataagtagg gcttcgtgac ggtgatcgag
      901 gcgatccgga cctgatcgga gaaaaggctc cgcagactgg gtacgatcac gacccgctcg
      961 gcccggagcg cgtctggagc cggccacccc tgtgaaccct tgatgcggag gcccagcacc
     1021 tccagtgccg accagcccac ccggagttcc gtgatgtcgc tgcccggccc caaggcttca
     1081 atcaccttcc ccttgagaac ggaaagcgcc acccggaatc caacaatccc gccggcgatc
     1141 agaatcacga aaaggacggc ggcggcggcg agtgtccacc ttttgccctt tcggctggtc
     1201 atggcttatt cctcttttcc cacacaatgc cgatggcggc gccgatccgg aacggactag
     1261 ccgccagcca tcctcagaat cgcatcccac gccgcgtcag gcacaggcat gacagacaga
     1321 cggggcaacc ggaccagctc gaaatccttg aacctccgat cggacttgat agcctccagg
     1381 gttactgctt gtctcagctt gcgtacggga acgacatcca cgaccacaat cttggggtcc
     1441 tgtcgctccg ggtccggata cggcgcgctg gcggctttgg ccaatcccac tgcagcccga
     1501 attgccccgg tgtgatagat gagtagctca tccccttttc gaatgctccg caggtgtttc
     1561 agggcgagag gattcgtcac gccgtcccag acagtcttcc tctcccgcat cagaccttcg
     1621 aatgaatatt ccccaggctc cgtcttcagc agccacctgt tcctcatggc ccagcctttc
     1681 tcctttcgtg gcgaccctga gacaccttgc gaaatatagc attcctagga agagttccgc
     1741 ggccatgcca accgaaatcc tgcctgttgc ataatccagc acacgggggt tgtcaatcgc
     1801 tagtgccccg ttctcgaagt tcgtcgacga attgccggg
//
LOCUS       DBNGZC010000480         2532 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2192627, whole genome shotgun sequence.
ACCESSION   DBNGZC010000480 DBNGZC010000000
VERSION     DBNGZC010000480.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2192627"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..>2532
                     /locus_tag="AC16G0_08455"
     CDS             29..>2532
                     /locus_tag="AC16G0_08455"
                     /inference="COORDINATES: protein motif:HMM:NF013106.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR5099683.1"
                     /translation="MTPQQVSISASFWRRTALYSREAVLRVLHRRITLPSTGPIPDLV
                     RAPEVGRTPLYRRHVPRWIRLSLLVGLGLALAAHELRTSALQSRLFSWWAGRVTYTLQ
                     PGPSAQITFPETGPFDQRLGYTRIHEFTRRLQERGYRVSAQVRFSEAAARLVSWGFPP
                     PYQEPSVAGLVIRDRTGALLYDSTLGHGVFHDIAEVPPLLVDTVLFVENRSLQEPLDP
                     RQNPVVDWARFGKAGALYAGRKLGLPLPLEGASTLSTQLEKFQHSRGGRTASALEKLR
                     QITSASLKAYRDGVDTRPERREILLRYLNEVPLAAAPGWGEVNGITDGLLAWFGDRPA
                     EVLGALASPSLTPEKVRVYKHVLALLCAVRAPTTYLVRNHAALEARVALYTELLQKAG
                     LIDPDLAHRLRLTPITYLREKISPPARLVLQRKAVSHLRAGLGQLLGEPNIYNLDRLH
                     LEVGSTLDLGLQQDVARLFDQIQDRGFLATHGLIGDHLLGAGDPRDVRYSFLLLERTP
                     LENLVRAHTDTLDTPFDINDGMKMELGSTAKLRTLAHYLELMTGLHAELSGLDADVLA
                     SRARSARDPLTRWAAETLRSQREITLDAFLRQALGRTYSASPREVFFTGGGVQVFANY
                     EHSEDGRVYSVQDGLIHSVNLVYIRLMRDIVQFHEARLPYDAQAVLSNPDDPVRGGML
                     EDIAATEAREALERAYREYRGLSPQEMEAHLLGRQVKDLRHQSMLFFAWHPGAGEEAL
                     GRWLDSRVGSVPPDELRRLMKAYGNPRLGLMDFAFLMGRRPLDVWAAGELAKTAKISL
                     DDLRFRCGETCRAAAAWLFRTRNRHAQDVRLRIRIE"
BASE COUNT          413 a          904 c          796 g          419 t
ORIGIN      
        1 gaaacacggc aacgtgagac aaggaaccat gactccccaa caggtctcta tttccgcgtc
       61 gttctggagg cgcacagccc tctattcccg cgaggcagtc cttcgagtcc tgcatcgtcg
      121 gatcacgctg ccctcaaccg gacccatccc tgacctggtc cgagcaccgg aggtgggccg
      181 cacccctctt taccgtcggc acgtcccgag gtggatacgg ctatcgctcc tggtaggact
      241 gggtctggcc ctggctgccc acgagttgcg gacctccgca ctccagtccc ggctcttctc
      301 ctggtgggcg ggacgtgtga cgtacaccct ccagcccggc ccgagcgcgc agatcacctt
      361 cccggagacc gggcccttcg atcagcggct gggctacacc cggatccacg agttcacccg
      421 tcggctgcaa gagcgcggct accgggtgag cgcccaggtg cgattctcgg aggcggcggc
      481 acggctggtc tcctggggct tccctccccc gtaccaggag ccgtcggtcg caggactcgt
      541 catccgtgac cggaccggcg cgctgctcta cgacagcacc ctgggccatg gcgtgtttca
      601 cgacattgcc gaggtccccc cgctcctggt ggacacggtc ctcttcgtcg agaaccggag
      661 cctccaagag cctctggacc cccgccagaa cccggtggtc gactgggctc gttttggaaa
      721 ggcgggcgcc ctctacgccg gtcgcaagct cgggctgccc cttccgcttg aaggggccag
      781 cacgctgtcc acacagctcg agaagttcca gcactcccgg ggcggtcgta ccgcatcggc
      841 cctcgagaag ctgcgccaga taacctccgc cagcctgaag gcgtaccggg atggagttga
      901 cacgcgcccg gagcgacgcg agatcctgct ccggtacctc aacgaggtcc cgctggcggc
      961 cgcgcctggc tggggcgagg tgaacggcat cacggatggg ctgcttgcct ggttcggcga
     1021 tcgacccgca gaggtactcg gtgccttggc ctcgcccagc ctcaccccgg agaaggtccg
     1081 cgtctacaag cacgtcctgg ccttgctgtg tgcggtgcgc gcaccgacca cctatctggt
     1141 gcgcaaccac gccgcgctcg aggctcgcgt ggcgctctac accgagctgc tgcaaaaggc
     1201 ggggctgatc gatccggatc tcgcgcaccg ccttcgcctg accccgatta catacttgcg
     1261 cgagaagatc agcccccctg cccggctcgt cctccagcgc aaggccgtca gccacctgcg
     1321 agccgggctg gggcagctcc tcggagagcc gaacatctac aacctggacc ggctccacct
     1381 cgaggtgggc agcacgctcg acctgggtct gcagcaggat gtggcgcggc tcttcgacca
     1441 gatccaggac cggggcttcc tggccacgca cgggctgatc ggcgaccatc tcctcggcgc
     1501 aggcgaccca cgggacgtgc gttacagctt cctgctgctc gagcgaaccc ctctggaaaa
     1561 cctggtccgc gcccacacgg acaccctcga cacgcccttc gatatcaatg acggcatgaa
     1621 gatggagctg ggcagtaccg ccaagctgcg caccctggcc cactacctcg agctgatgac
     1681 cggcctgcac gcggagcttt ccgggctgga cgccgacgtc ctcgcctcgc gcgcccgctc
     1741 ggcccgcgat ccgctcaccc gctgggccgc cgaaaccctc cggagccaga gagagatcac
     1801 cctggacgca ttccttcggc aggcgctcgg ccgcacctac tcggcgagcc cgcgagaggt
     1861 gttctttacc ggaggcggcg tccaggtgtt tgccaattac gaacacagtg aggacggccg
     1921 tgtctactcc gtgcaagacg gactgatcca ctcggtcaac ctcgtgtaca tccgcctgat
     1981 gcgggacatc gtgcagtttc acgaggcgcg cctcccgtat gacgcgcagg cagtgctctc
     2041 caaccccgat gaccccgtgc ggggtggcat gctggaagac atcgcggcca cggaagccag
     2101 agaggcccta gaaagagcct atcgcgagta ccggggcctc tccccccaag agatggaggc
     2161 ccatctcctg ggccgacagg tgaaggatct gcgccaccag tcgatgctct tcttcgcctg
     2221 gcaccccggc gcaggggagg aggcgctggg ccgctggctc gattcacgcg tgggatcggt
     2281 gccgcccgac gagctgcggc ggctgatgaa ggcctacggg aacccgcggc tgggcctgat
     2341 ggacttcgcc ttcctgatgg gccgtcgccc gctcgacgtg tgggctgccg gcgaactggc
     2401 aaaaacggcg aagatttccc tggatgacct ccggttccgc tgcggcgaga cctgccgcgc
     2461 cgccgcggcc tggttgttca ggacccgcaa tcgtcatgcg caggatgtgc gcttacgcat
     2521 ccgcatcgag ca
//
LOCUS       DBNGZC010000481         1476 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2096074, whole genome shotgun sequence.
ACCESSION   DBNGZC010000481 DBNGZC010000000
VERSION     DBNGZC010000481.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2096074"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="AC16G0_08460"
     CDS             <1..1017
                     /locus_tag="AC16G0_08460"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5099684.1"
                     /translation="ELQKMIFGPEFSKRFPNVEINAVGSGPGDPGSRVIFQKLKSEKD
                     ANVANYDIDVAIVHQSIMPDMIQNDLLAKYGPEISTYKMMSAANGRNALGFNVEGYVM
                     PMFQSQVVVAYNPDMVSNPPNTFEELVAWVKANPRKFGYNGVKGGMSGTGFVTGWAYW
                     KTGKYEQYAMGAYEKSAQAGWPAAFKELKGLPVTMTTGNNDTLDKLNRGEIAMGPVWV
                     DMLINMKNEGRMDPKIKMKLISPGLPGQPMYLVVTKKAANYEMAKKFVEFITSPEQQA
                     KVIVERQGWLPGIDAKYVMPEVSPKAKEMIFRDLPADVLEKNGLVFPQQQYFKDLLTA
                     YEEN"
     gene            1077..>1476
                     /locus_tag="AC16G0_08465"
     CDS             1077..>1476
                     /locus_tag="AC16G0_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099685.1"
                     /translation="MRLSLRIACLVIALPPLAIVIWLFFIPFLSSAHLSLIRGDQWSL
                     RNYFLVWRLYRYDILYTLWIAFVSLVAVLGIAVFLCGYLRIYTNKAVEFLFKIPLFVP
                     FVVVGHGMRVLLAPRGTLNSALAQVGLVSLA"
BASE COUNT          293 a          447 c          475 g          261 t
ORIGIN      
        1 gagctgcaga agatgatctt tggcccggag ttcagcaaga gattcccgaa cgtggagatc
       61 aacgccgtcg gcagcgggcc gggggatccg ggaagccggg tgatcttcca gaaactcaag
      121 agcgagaagg acgccaacgt cgccaattat gacatcgacg tggccatcgt gcaccaaagc
      181 atcatgccgg acatgatcca gaacgacctg ctggcgaagt acggcccaga gatcagcacc
      241 tacaagatga tgtccgccgc caacggccgc aacgctctgg gcttcaacgt cgaaggctac
      301 gtcatgccca tgttccagag ccaggtggtc gtggcctaca atcccgacat ggtgtcgaac
      361 ccgccaaaca ccttcgagga attggtggcc tgggtcaagg cgaatccgcg gaagttcggc
      421 tacaacgggg tcaagggcgg catgagcggc accgggttcg tcaccggctg ggcctactgg
      481 aagaccggga agtacgagca gtacgccatg ggggcctacg agaagagcgc gcaggcgggc
      541 tggcccgcgg ccttcaaaga gctgaaggga ctgcccgtga ccatgacgac ggggaacaac
      601 gacaccctgg acaagctgaa ccggggcgag atcgccatgg ggccggtctg ggtggacatg
      661 ctgatcaaca tgaagaacga gggccgcatg gaccccaaga tcaagatgaa gctgatctcg
      721 cccggcctgc cgggtcagcc gatgtacctg gtggtcacca agaaggcggc caactacgag
      781 atggccaaga agttcgtcga gttcatcacc tcgcccgagc aacaggccaa ggtgatcgtg
      841 gagcggcagg ggtggctccc gggcattgac gccaagtatg tgatgccgga ggtgtcgccc
      901 aaggccaagg agatgatctt ccgcgacctg ccggcggatg tcctggagaa gaacggcctg
      961 gtcttcccgc agcagcagta tttcaaggac ctgctgacgg cgtacgaaga gaactgaggc
     1021 cccggaccca cggagagagg cgggcgaggg gggcgtagcc ccctcgccct ttccgtatgc
     1081 gactctcact gcgcatcgcc tgtctggtca tcgcgctgcc cccgctcgcc atcgtcatct
     1141 ggctgttctt cattcctttt ctttcctcgg cccatctcag cctgatccgc ggggaccagt
     1201 ggagtctgcg gaactacttc ctggtctggc ggctgtaccg ctacgatatc ctgtacacgc
     1261 tctggatcgc gttcgtcagc ctggtcgccg tcctcggcat cgccgttttc ctgtgtgggt
     1321 acctgcgcat ctataccaac aaggcggtcg agttcctgtt caagatcccg ctcttcgtgc
     1381 cgttcgtcgt cgtcgggcac gggatgcggg tgctgctggc gccgcggggg acgctcaact
     1441 ccgccctcgc ccaggtgggc ctggtgagcc tggccg
//
LOCUS       DBNGZC010000482         2392 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1017665, whole genome shotgun sequence.
ACCESSION   DBNGZC010000482 DBNGZC010000000
VERSION     DBNGZC010000482.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1017665"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1598
                     /locus_tag="AC16G0_08470"
     CDS             <1..1598
                     /locus_tag="AC16G0_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5099686.1"
                     /translation="DARVLVAEGQEEVDKALAIKARCPKLEKIIWDDPRGMRDYDDPA
                     LVQFTQVQELGRKLLARDPDRFESLVERGQGNDVCLLFYTSGTTAKPKGALLTHTNML
                     KMGQNLMRVDPCLASDDFVSFLPMAWIGEQMMSISCGLQAGFTLNFPEEPETVIANLR
                     EIGPHTVFSSARFYEQMVRTVQVKYLDASWLKRKVYETALAIGHRLADMKLARRCIPW
                     MWRVLGVLADQAVHRKLRDHLGLSRIRNAYSGGSALGPDYFRFFHAIGVNLKQIYGQT
                     EIAGISVVHRSGDIKFDTVGTPIPETEVRITPEGEIISRSPSVFQGYYKDPAETAKAL
                     RNGWLHSGDTGFIDADGHLVFFDRTKDVFRLSDGSRFSPMYLESRLKFSPFVKDAWVL
                     GHERPHAAAVICIDGSVVGKWAEDHRIAYTSYSELSQHPQVYALIQAAIQQVNAQLPE
                     AARVRRFLNLHKEFDADDEELTRTRKIRRAFLEERYRDLVEALYGGAGHVGVGTTITY
                     EDGRVVEVKAELRIVDVSDGGGH"
     gene            1602..>2392
                     /locus_tag="AC16G0_08475"
     CDS             1602..>2392
                     /locus_tag="AC16G0_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5099687.1"
                     /translation="MDLFLQLLITGVMVGSIYALVALGWTLIYKCSGVLNLAMGELTL
                     LGAYVCLTFYSWGIPFPLAVVGMLLIGFLLGLLTERLFLRRMVGESVLAIIMVTVGLS
                     FFFRGIVAITWGTDTRVFAPPVFSQEPIQLGRVIVGQVYVWSFLAALAMLVVFVAFFK
                     YTRWGLSMQATADDEMASLSLGVSAHRVYALAWAIAFMAAGVGGTLLGNINGLNISVG
                     SLGLLVLPAVVLGGLNSVPGAIVGGIAIGVLQNLSDGYLSRFTPGG"
BASE COUNT          395 a          768 c          778 g          451 t
ORIGIN      
        1 gcgatgcccg cgtcctggtg gcagagggcc aagaagaagt ggacaaggcc ctggccatca
       61 aggcgcgctg cccgaagctc gagaaaatca tctgggacga tccgaggggg atgcgagatt
      121 acgacgatcc ggcgctcgtc cagttcaccc aggtccagga gctgggccgc aagctcctcg
      181 cgcgcgaccc ggatcgcttc gaatccctgg tcgagcgagg tcaggggaac gatgtctgcc
      241 tgctcttcta cacctccggg accacggcca aacccaaggg agcgctcctc acgcacacca
      301 acatgctgaa gatgggccag aacctcatgc gggtggaccc ctgcctcgcg agcgacgact
      361 tcgtctcctt cctgcccatg gcttggatcg gggagcagat gatgtccatc tcctgcggtc
      421 tgcaggcggg cttcaccctg aacttccccg aggagcccga gaccgtcatc gccaatctgc
      481 gtgagatcgg gccccacacc gtcttctcct cggcccgctt ctacgagcag atggtgcgca
      541 cggtgcaggt gaaatacctg gatgccagtt ggctcaagcg caaagtgtac gagacggcgc
      601 tggccatcgg tcatcggctg gcggatatga agctggccag gcgatgcatc ccctggatgt
      661 ggcgcgtcct gggggtgctg gcggatcagg ctgttcaccg aaagctccgg gaccacctgg
      721 ggctctcgcg gattcgcaat gcgtacagtg gcggctccgc tctggggccg gactacttcc
      781 ggttcttcca tgccatcggc gtgaatctga agcagatcta cggccagacc gagatcgccg
      841 gcatctccgt ggtgcatcgg agcggcgaca tcaagttcga caccgtcggc acccccattc
      901 cggagaccga ggtccggatc acgcccgagg gggaaatcat ctcccggagc ccgtcggtgt
      961 ttcagggcta ctacaaagat ccggcggaga ccgccaaggc tctgcggaac ggctggctcc
     1021 actccggcga cacgggattc atcgacgcgg acggccatct ggtcttcttc gaccggacca
     1081 aggacgtctt ccggctttcg gacggaagcc gattctcccc catgtacctg gagagccgcc
     1141 tcaagttcag cccattcgtc aaggacgcct gggtcctggg tcacgagcgc ccccacgccg
     1201 cggccgtgat ctgcatcgac ggatcggtgg tgggcaagtg ggccgaggac caccgcatcg
     1261 cctacaccag ctactccgag ctgtcccagc acccgcaggt gtacgccttg atccaggccg
     1321 ccatccagca ggtgaatgcc cagttgcctg aggcagcacg ggtgcgacgc ttcctcaacc
     1381 tccacaagga attcgatgca gacgacgagg agctgacgcg gacgcgaaag atccggcggg
     1441 ccttcctgga agagcgctat cgcgacctgg tggaggccct gtacgggggt gccggacacg
     1501 tgggcgtcgg gaccaccatc acctacgagg acggccgggt ggtcgaggtg aaggcggagc
     1561 tgcgaatcgt ggacgtgtcg gatggcgggg ggcactgacc catggacctc tttctgcaac
     1621 tgctcatcac cggcgtcatg gtggggagca tctacgcgct ggtggcgctg ggctggaccc
     1681 tcatctacaa gtgctcgggc gtcctgaatc tggccatggg ggaattgacc ctcctgggag
     1741 cctacgtctg cctcaccttt tactcctggg ggatcccatt tcccctggcg gtggtgggga
     1801 tgctcctcat cggctttctc ctggggctgc tcacggagcg gttgttcctc cgccggatgg
     1861 tgggggaatc cgtcctggcg atcatcatgg tgacggtggg tctgtccttc ttcttccggg
     1921 gcatcgttgc catcacctgg gggacggata cccgggtctt cgcgcctccg gtcttcagcc
     1981 aggagccgat ccaactgggt cgggtcatcg tggggcaggt ctacgtctgg agcttcctcg
     2041 ccgcccttgc catgctggtg gtattcgtgg ctttcttcaa gtacacccgc tgggggctgt
     2101 ccatgcaagc caccgcggat gacgagatgg cttccctctc gctcggtgtg agcgcccatc
     2161 gcgtctatgc cctggcctgg gccatcgcct tcatggcggc cggcgtcggc gggacgctcc
     2221 tggggaacat caacggcctg aacatctcgg tgggctccct gggcctcctg gtcctgccgg
     2281 cagtcgtcct gggtgggctg aactcggtgc ccggggccat cgtgggcggc atcgccatcg
     2341 gagttctgca gaacctgtcc gacggctacc tcagccggtt caccccggga gg
//
LOCUS       DBNGZC010000483         2341 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1410428, whole genome shotgun sequence.
ACCESSION   DBNGZC010000483 DBNGZC010000000
VERSION     DBNGZC010000483.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1410428"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="AC16G0_08480"
     CDS             <1..300
                     /locus_tag="AC16G0_08480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HIR5099688.1"
                     /translation="IYTGLHQTVEQVAAAALQESADVVALSILTGAHLSLTRKLIARL
                     EALEIRKDVVLAVGGTIPPADVSSLRDLGVEGVFPTGTPLAEIVGWFRALAEGRT"
     gene            300..1202
                     /locus_tag="AC16G0_08485"
     CDS             300..1202
                     /locus_tag="AC16G0_08485"
                     /inference="COORDINATES: protein motif:HMM:NF012758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099689.1"
                     /translation="MWLLQGQVRRIFRECGLEEAAPDAGADVRLTLEVEPAEGMIRIG
                     AVRAAGPPSGNAPEVAWVGVDPRYGLWDYQARDLLLRVGLRGWVRVGPPLIRRLWQVF
                     REYELLRLETELLGAGDGPRFSGGRMLCDDNALGRNERLSRLREAVAHEPSHMMRSHG
                     LEYVELDGEIGLLSVGAGETMATMDLLEAAGGRAACFMDVSGGFGPDAVTAALERVRG
                     LPHIRVMMVNVFGGVTRVDDVAASIATALERIPDFSLPLVIRLEGNRADRGRELLSRI
                     GLQCFTLLRDAVEAAVALARKEQA"
     gene            1199..2074
                     /gene="sucD"
                     /locus_tag="AC16G0_08490"
     CDS             1199..2074
                     /gene="sucD"
                     /locus_tag="AC16G0_08490"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR5099690.1"
                     /translation="MSILLDGTTRVIIQGVTGREGSFHAQLMREYGTRIVGGVTPGKG
                     GQAHDGMPVFDTVAEARRKTGAEASITFVPTSAAVDSLVEAIRAGLRLVVCTTDGIPV
                     HDMLPVSVMAREGACSLIGPNCPGLITPGRSKLGFMPSHVYSPGPVGVMAKSGSLSYE
                     VAYELTRAGLGQSTVVGIGGDPVKGCSFVDLLPLFAEDPETQAIVLVGEIGGTDEEQA
                     AGWLERSRIDKPVIAYLVGRTAPPFVKLGHPATLFVRNDRNYAEKVERLRAAGAMMAF
                     SPWDVAACAHAIFGR"
     gene            2079..>2341
                     /locus_tag="AC16G0_08495"
     CDS             2079..>2341
                     /locus_tag="AC16G0_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014824551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR5099691.1"
                     /translation="MSGNVLYDEDGGVGTVTVNRPEVHNALNMETIRELRELCLQLRA
                     EDRLRAMILTGAGEKTFLSGGDLKEFQHLKTVEEARRMISSMK"
BASE COUNT          366 a          691 c          893 g          391 t
ORIGIN      
        1 atctacaccg gattgcacca gacggtggag caggtggcgg cggcagccct gcaggagtcg
       61 gcggatgtcg tggcgctcag catcctcacc ggggcccacc tctccttgac gcggaagctg
      121 atcgcacggc tggaagccct ggagatccgg aaggacgtgg tgctggcggt tggcgggacg
      181 attcctccag cggatgtgtc gagtctgcgt gatctcgggg tggagggtgt ctttcccacc
      241 ggaacgccgc tggcggagat cgtcgggtgg ttccgggccc tggccgaggg caggacgtga
      301 tgtggctgct ccagggacag gtccgacgga tttttcgtga gtgcggtctg gaagaggcgg
      361 ctccagatgc cggggcggat gtgcggctga cgctggaggt ggagccggcc gaggggatga
      421 tccggatcgg ggccgtgcgg gcggctggtc cgccttcggg gaacgctcca gaggtggcat
      481 gggtgggcgt ggatccccgc tacggcctct gggattacca ggcacgcgac ctcttgctga
      541 gggtgggtct gcggggctgg gtgcgtgtgg gtccaccgct gatccgtcgg ttgtggcagg
      601 tgtttcggga gtacgaactg cttcgcctgg aaacggagct gttgggagcg ggcgacggcc
      661 caaggttctc gggcggccgg atgctctgtg acgacaacgc cctcggccgg aacgaacgcc
      721 tgtcacggtt gcgggaggcg gtcgcgcacg agccctccca catgatgcga tcacacggac
      781 tcgagtacgt ggagctggac ggcgagatcg gcctgctttc ggtgggggcc ggggaaacca
      841 tggccaccat ggatctgctg gaggccgcgg ggggccgcgc cgcctgcttc atggacgtga
      901 gcggcgggtt tgggcccgat gccgtgaccg ccgccctgga gcgcgtccgc ggcctgccgc
      961 atatccgggt gatgatggtc aatgtcttcg gaggcgtgac ccgcgtggac gacgtggccg
     1021 cgtccatcgc gaccgccttg gagcggatcc ccgacttctc tcttcctctt gtgatccgcc
     1081 tggagggaaa cagggccgac cgcggccggg agctgctcag ccggatcgga ttgcagtgct
     1141 tcacgctcct ccgggacgcg gtggaggccg cggtggcctt ggccaggaaa gagcaagcgt
     1201 gagcatcctg ctggatggga cgacacgggt catcatccag ggggtgacgg gacgggaggg
     1261 gtccttccac gcccagctca tgcgcgaata cggtacccgg atcgtgggag gggtcacgcc
     1321 cgggaaaggc ggtcaagcgc acgacgggat gcccgtcttc gacacggtgg ccgaagccag
     1381 gcggaagacg ggtgcggagg cgagcatcac cttcgtgccc acgtcagcag ccgtggacag
     1441 cctggtggag gccatccggg ctggcctccg cctggtcgtc tgcacgaccg atgggattcc
     1501 ggtgcatgac atgctgccgg tcagcgttat ggcccgggaa ggtgcctgca gcctgatcgg
     1561 gcccaactgc ccggggctga tcacgcccgg caggagcaag ctcgggttca tgccgagcca
     1621 tgtctacagc cccggtcctg tcggtgtgat ggccaagagc gggtccctgt cctacgaggt
     1681 ggcctacgag ctgacccgag cgggccttgg ccagtccacg gtcgtgggga tcgggggcga
     1741 tcccgtgaag ggctgctcct tcgtggacct gctccccctc ttcgcggagg accccgagac
     1801 ccaggccatc gttctggtcg gggagatcgg tgggaccgac gaggagcagg ccgccggctg
     1861 gttggagcgg agccggatcg acaagccggt gatcgcctac ctggtcggac gcacggcgcc
     1921 gcccttcgtg aagctggggc atccggcgac cctcttcgtc cggaacgatc ggaactacgc
     1981 cgagaaggtc gagcggctac gagccgccgg tgccatgatg gccttcagtc cctgggacgt
     2041 ggcggcctgc gcccatgcga tcttcgggag gtgaaggggt gagcgggaac gtcctgtacg
     2101 acgaggacgg gggggtgggc acggtgaccg tcaatcggcc cgaggtccac aatgccctga
     2161 acatggagac gatccgggaa cttcgagagc tgtgcctgca actgcgggcg gaggatcgtc
     2221 tccgggccat gatcctgacc ggggcgggag agaagacctt tctctccggg ggggacctga
     2281 aggaattcca gcacctcaag acggtggaag aggcccgccg catgatctcc agcatgaagg
     2341 a
//
LOCUS       DBNGZC010000484         3505 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2192777, whole genome shotgun sequence.
ACCESSION   DBNGZC010000484 DBNGZC010000000
VERSION     DBNGZC010000484.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2192777"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..1233)
                     /locus_tag="AC16G0_08500"
     CDS             complement(229..1233)
                     /locus_tag="AC16G0_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099692.1"
                     /translation="MRLLEVKGLQKFFPIRRGILQREVGRVRAVDDVSFYVEKGETLS
                     LVGESGCGKTTTARCILRAIPPTAGQILFRPEDGTVVDVATLPKGQLRPLRRQMQMIF
                     QDPFSSLNPRRTLLDIVAEPLVANKVGTHHEQVERVAELLRLVGLRPEYVRRYPHAFS
                     GGQRQRIGIARALALNPSLVVADEPVSALDVAVQAQILNLMLRLQSQHGLTYLFVAHD
                     LSVVKHVSDRVAVMYVGRIVEMAATQRLFATPLHPYTEALLSAVPKPDPRLRSQRIVL
                     EGEVADPANPPSGCYFHPRCRYAADVCRTEKPQLQEIRPGHFVSCHRAGDLNLRGVVA
                     "
     gene            complement(1248..2237)
                     /locus_tag="AC16G0_08505"
     CDS             complement(1248..2237)
                     /locus_tag="AC16G0_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099693.1"
                     /translation="MRDLKTYFFPDEGTVRAVDGASFDVDSRKTLGIVGESGCGKSVT
                     ARSILRIVEQPGRIVAGSILLRRNGSHGDASELDLTQLDPGGQEMRRIRGGEIGLVFQ
                     EPMTSFSPVHTIGNQIVETIRLHHQVSEPAARQQAIEALRSVGIPKPERRIDEYAFEL
                     SGGLRQRAMIALALSCRPRILIADEPTTALDVTTQAQILDLLRQLQDREGMAIILITH
                     NLGVVAEMCDAVVVMYLGRVVERGPVDAIFHAPKHPYTKALLRSIPSIHATVRTELPT
                     IAGSIPHPYNRPVGCPFHPRCPDFMPGTCDREEPSLRPAGEGQDVSCFLYHPA"
     gene            complement(2378..>3505)
                     /locus_tag="AC16G0_08510"
     CDS             complement(2378..>3505)
                     /locus_tag="AC16G0_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012654021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099694.1"
                     /translation="SGAGEPEAPASFAEERVSVATQWQLMWWRFKKHRLAMVSTAVVL
                     LFYLVVLLADFLAYADPTVSEAQRSLLPPQSIHWFDGGRLAPHVFGLTGMRDPNTFKR
                     VYTPDPEKKIPVRFFVRGFEYSLFGLIPATLHVIGVEGTTPEESLFLLGTDEQGRDVW
                     SRLMYGTRTSLTIGLAGVLLSLILGILLGGISGLYGGVLDTVIQRVIEILRSIPTIPL
                     WMGLAAALPNDWSVMQVYFAITLILSLIGWTELARVVRGRFLSLRQEDFVMAAELCGC
                     NRMRIIFTHLVPSFLSHIIAATTLAIPAMIISETSLSFLGLGLRPPAISWGVLLQQAQ
                     NIQALAISPWLLIPSVPVIIVILAFNFMGDGLRDAADPYGH"
BASE COUNT          611 a         1160 c         1206 g          528 t
ORIGIN      
        1 gatccagcat gtggtcgcgg tagatgatgt ccaggtcgcg gggcacgatg acgtgatcgg
       61 tgacccacac cgagtcaagc ccccgctcct ctgcccgccg gacgacctcc aggatgcggc
      121 cgacctcgat cggccgcccg tagtgcggga tgcagatgcc gagcttcatg gctcttccct
      181 cctgttcccg ccgaggatcg agacgcggcc gcaacgcgac cggagccgtc aggcgaccac
      241 ccctcgcagg ttcagatccc ccgcccggtg acagctcacg aaatggcccg gccggatctc
      301 ctgtagctgc ggcttctctg tccggcagac gtccgccgca tagcgacagc gcgggtggaa
      361 atagcagcct gacggcgggt tcgcgggatc cgcgacctcc ccctccagca cgatgcgctg
      421 ggaccgcagc cgaggatccg gcttcgggac cgccgacagg agcgcctcgg tgtaggggtg
      481 caggggggtc gcgaagaggc gctgcgtcgc ggccatctcg acgatccggc cgacgtacat
      541 gactgccacc cggtcgctca cgtgcttgac cacggagaga tcgtgggcca cgaagagata
      601 cgtcagaccg tgttgggact ggaggcgcag catgaggttg aggatctggg cctggaccgc
      661 cacgtccagg gccgagaccg gctcgtccgc gaccaccagg ctgggattca gggccagggc
      721 ccgggcgatc ccgatccgtt ggcgctgtcc ccccgaaaag gcgtgcgggt accggcgcac
      781 gtactcgggc cggaggccaa ccagccgcaa gagctccgcc acccgctcca cctgctcatg
      841 gtgcgttccc accttgttcg ccaccagcgg ctcggccacg atgtccagga gcgtgcgccg
      901 gggattgagg gaagagaagg ggtcttggaa gatcatctgc atctggcggc gcagcggccg
      961 gagctggcct ttgggcaggg tagccacatc caccaccgtc ccatcctcgg gccggaagag
     1021 gatctgaccg gcggtgggcg gtatggcccg caggatgcac cgagcggtgg tcgtcttgcc
     1081 acagccactc tcccccacca gcgagagagt ctcccccttc tcgacataga agctcacgtc
     1141 gtccacggcg cggacgcgac ccacctcccg ctggaggatc cctcggcgga tgggaaagaa
     1201 cttctgcagg cccttcactt ccagcaggcg cacggaggct cccatcgtca ggccgggtga
     1261 tacaggaagc aactcacgtc ctggccctcg cccgccgggc gcaggctcgg ctcctcccgg
     1321 tcgcaggtgc cgggcatgaa atccggacag cggggatgga acgggcaccc aaccggccgg
     1381 ttgtaggggt gggggatgga gcccgcgatg gtcggcagct cggtgcgcac ggtcgcgtgg
     1441 atgctcggga tggagcgcag gagcgccttg gtgtaggggt gcttcggcgc gtggaagatg
     1501 gcatccacgg ggccccgctc caccacccga ccgagataca tgaccaccac cgcatcgcac
     1561 atctcggcca cgacccccag attgtgggtg atgaggatga tcgccatccc ctcccgatcc
     1621 tggagctggc ggagcagatc caggatctgg gcctgggtgg tgacatccaa ggccgtggtc
     1681 ggctcgtcgg cgatcaggat gcgcgggcgg caggaaaggg ccagggcaat catggctcgc
     1741 tggcgcagcc cgccggacag ctcgaaggcg tactcgtcga tccgccgctc gggcttgggg
     1801 atgccgacgg agcggagcgc ctcgatggcc tgctgccgcg cagccggctc actcacctgg
     1861 tgatgcagcc ggatggtctc gacgatttgg ttgccgatgg tgtggacggg gctgaaggac
     1921 gtcatcggct cctggaagac caggccgatc tcgccgcccc ggatgcggcg catctcctgg
     1981 cctccggggt cgagctgggt caggtccagc tcggacgcat cgccatggct gccattgcgc
     2041 cggaggagga tggacccggc cacgatccgg ccgggctgct ccacgatgcg caggatggag
     2101 cgggccgtga cgctcttgcc gcagccgctc tcccccacga tgcccagggt cttccgcgag
     2161 tcgacatcga agctggcgcc gtccaccgcc cggaccgtgc cctcgtcggg gaagaagtag
     2221 gttttcagat cgcgcaccga caggatcggc ggcgcactgc caaggctggt cacatcaacc
     2281 acccgctgct ctctcttccg cccttatcgc catgcctgtc gggtgcccat cgacgacggc
     2341 aaaggtctcg ttgcctgtgg ctgtccctac cgcccggtca atgtccgtag ggatccgccg
     2401 catcccgcag cccgtcgccc atgaagttga atgccaggat cacgatgatg accgggacgg
     2461 acgggatcaa gagccagggc gagatggcca gggcctggat attctgcgct tgctgcaaga
     2521 ggacccccca gctgatggcc ggcggccgga gccccaggcc caggaaggag agggaggtct
     2581 cgctgatgat catggccggt atggccaggg tcgtggccgc gatgatgtga ctcagaaagg
     2641 acggcacgag gtgcgtgaag atgatccgca tccggttgca gccgcagagc tccgccgcca
     2701 tcacgaagtc ctcctgacgc agcgacagga agcggccgcg gaccacccgc gccagctccg
     2761 tccagccgat cagcgagagg atgagggtga tggcgaaata gacctgcatg acgctccaat
     2821 cgttcggcag ggccgccgcc agacccatcc acagcgggat ggtcgggatg gagcgcagga
     2881 tctcgatcac acgctggatg actgtatcca ggaccccgcc gtagagccct gagatcccgc
     2941 ccaggaggat gcccaggatc aggctgagca agacaccagc caggccgatg gtgagcgacg
     3001 tccgggtccc gtacatcagg cgggaccata cgtcacggcc ctgctcgtcg gtccccagca
     3061 ggaacagcga ctcttcgggc gttgtcccct cgacgccgat gacgtgcagg gtggccggga
     3121 tcaggccgaa cagggagtac tcgaagcccc ggacgaagaa gcggaccggg atcttcttct
     3181 ccgggtcggg cgtgtacacg cgcttgaagg tgttggggtc gcgcatgccc gtgaggccga
     3241 agacgtgcgg ggcgagccgt cccccgtcaa accagtggat gctctggggc gggaggaggg
     3301 accgctgcgc ctcggagacc gtcgggtccg cgtaggccag gaaatcggcc agcagaacca
     3361 ccaggtagaa gagcagcacc accgcagtgc ttaccatggc gaggcggtgc ttcttaaagc
     3421 gccaccacat gagctgccac tgcgtggcga cggagacccg ctcctcggca aaggatgccg
     3481 gggcctccgg ctcgcccgcg ccgct
//
LOCUS       DBNGZC010000485         2866 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_41681, whole genome shotgun sequence.
ACCESSION   DBNGZC010000485 DBNGZC010000000
VERSION     DBNGZC010000485.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_41681"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="AC16G0_08515"
     CDS             complement(<1..581)
                     /locus_tag="AC16G0_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR5099695.1"
                     /translation="MGGQSRHRLLVTFVLLAVLWLLAVPAMAQQAPMAPAPTTTPAAP
                     AAVTVSQAAPPPKIDSGDTAWMLVSSALVLMMTAPGLALFYGGMVRRHNVLGTIMHSF
                     IIMAVVSLQWVLWGYSLSFGPDVHGLIGNLSWFGLRDVGLTPNADYAATIPHQAFMIY
                     QLMFAIITPALICGAFAERMKFSAMAIFLSLWS"
     gene            complement(1079..1285)
                     /locus_tag="AC16G0_08520"
     CDS             complement(1079..1285)
                     /locus_tag="AC16G0_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099696.1"
                     /translation="MGLCTILNKDTSLAAIIGVFELTRAAEETIERTFRSFEIYPLAA
                     AIYFCISFPLSLLAQRAESRLRHS"
     gene            complement(1381..2115)
                     /locus_tag="AC16G0_08525"
     CDS             complement(1381..2115)
                     /locus_tag="AC16G0_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR5099697.1"
                     /translation="MSFDWSVIASNWKFLILQGLLGFGSFSGGTLRLAIPAIVLGFLL
                     GIVIGLCRLSHRPWIHYPAVLYVEFFRGVPLVMVIFWFWFLIPQITGERLPEYTVALT
                     AFVVFEAAYLGEIVRAGIQSVPRGQIEAATAEGLSYAQMMGYVILPQAIRNMIPSLVT
                     QFIVLFKDTSLASIIGMMDLTKAAQTVSQREIRPFEMYLFIAVVYWIFAYSMSCFSRW
                     LEVKMSPERAPRKRATLPQATEAAAA"
     gene            complement(2112..>2866)
                     /locus_tag="AC16G0_08530"
     CDS             complement(2112..>2866)
                     /locus_tag="AC16G0_08530"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR5099698.1"
                     /translation="SMPLTAFLGMQYDFQWSVLWTGEHGQWLLQGIWTTLRLSALSWI
                     LASFLGILSGSLRTVPFAPLRALATCYVEFFRNVPLLVWLFFWYFGAPQVLPRAAQDW
                     LNHHGPEFWSAVVGLSVYHGARMSEVIRAGIQSIPKTLLEASVATGLSVRQAYRLIII
                     PIALRLIIPPATNESLNLLKNSSLALTIGVAELTFMSRQIETYTAKAFEALTAGTLIY
                     LILCLGIAAVMGRVEQRYRIPGLIARAGRAEH"
BASE COUNT          591 a          820 c         1013 g          442 t
ORIGIN      
        1 atcgaccaga gagaaagaaa gatcgccatg gcgctgaact tcatgcgttc ggcgaaggcg
       61 ccgcagatga gggccggcgt gatgatggcg aacatgagct ggtagatcat gaacgcctga
      121 tgggggatgg tcgccgcgta gtcggcgttg ggcgtcaggc cgacgtccct caggccgaac
      181 cacgacaggt tgccgatcag accatggacg tccggcccga aggagaggct gtagccccag
      241 agcacccact ggaggctgac caccgccatg atgatgaagc tgtgcatgat ggttcccagg
      301 acgttgtggc gccggaccat gcctccatag aagagggcga gtcccggagc ggtcatcatc
      361 aggaccaagg ccgaagagac gagcatccag gcggtgtccc cgctgtcgat cttgggcggg
      421 ggcgcggcct gtgataccgt cacagcggct ggagcggcag gcgttgtggt cggcgccgga
      481 gccatggggg cctgctgtgc catcgcagga acggccagca gccagagcac tgccaggagc
      541 acgaaggtca ccagaagccg atgccgagac tgccctccca tgatgactcc ctccttgagg
      601 aacacggtcc accgttcttt gcaagcggtg gacgaggctg acgctaccag agatcgtgtg
      661 ccggtcgatg tatcccatcg agccctcgga tccgtgggtg gaagtactca atggccagac
      721 gattcaggga agtgacggtg aatctcgatg gtggagcgtg ccgctcggct tgccatcccc
      781 aggcggatag ggggacaaca ctgcggtcgg ctatgaatcg aactgcgctg ctcatgtgtg
      841 cactccgttg tgcagatggt ccctgcgggt aacagcatgc ggcgtgcctg tggaggggaa
      901 ggccggaccg ggaccggcaa agtcatgcaa agtgtgaaga accctagccg aacagggagg
      961 ccggtgccgg gacccgctgg aattggtggc accactcctg cctccggaaa cggcacgtgc
     1021 agacgggggt gcctatcctg tgggcggaac gccgtggcca gacaccgtgc agccaccgct
     1081 aggagtgccg caggcgcgac tcggctcgtt gggccaggag ggagagagga aagctgatgc
     1141 agaagtagat ggccgccgcc agagggtaga tctcgaagga ccggaaggtg cgctcgatgg
     1201 tctcctcggc ggcccgtgtg agctcgaaga caccgatgat ggctgccagg gacgtgtcct
     1261 tgttcaggat cgtgcacaga cccacgaagg gtggaagcct acggcggacc gctcaagggg
     1321 cgaagcgcag gagaccggcc atgccgccgg cggaggtgcc cagcagcatg acggcggggg
     1381 tcaggcggct gcggcctccg tggcctgggg caacgtggcg cgcttcctgg gtgcgcgctc
     1441 gggcgacatc ttgacctcca gccatcggct gaagcaggac atgctgtagg cgaaaatcca
     1501 gtacaccacg gcgatgaaga ggtacatctc gaaggggcga atctctcgct ggctgaccgt
     1561 ctgggccgcc ttggtgagat ccatcatgcc gatgatggag gcaagggacg tgtccttgaa
     1621 gaggacgatg aactgggtca ccagcgaggg gatcatgttc cggatggcct gggggaggat
     1681 gacgtagccc atcatctggg cgtaactcaa cccctcggcc gtggccgcct cgatctggcc
     1741 gcgggggacc gactggatcc cggcccggac gatctcgccg agataggcag cctcgaagac
     1801 cacgaaggcg gtcagggcca ccgtgtattc cggcagacgt tctcccgtga tctgcgggat
     1861 gaggaaccag aaccaaaaga tcaccatcac cagggggacg cctctaaaga actcaacgta
     1921 caggacggct gggtagtgga tccaggggcg atgggacagc cggcagagcc cgatgacgat
     1981 gcccagcagg aatcccagga cgatggcggg aatcgccagg cgtagcgtcc ctccgctgaa
     2041 gctcccgaac ccgaggaggc cctggaggat caggaacttc cagttgctgg caatgacgct
     2101 ccagtcgaag ctcaatgttc ggccctcccg gcgcgggcga tgaggcccgg gatacggtac
     2161 cgctgctcca cccggcccat gacggcagcg atgccgagac acaggatcag gtagatcagc
     2221 gtcccggcgg tcagggcctc gaaggctttg gcggtgtacg tctcgatctg gcgactcatg
     2281 aaggtgagtt cggccacccc gatcgtcagg gccagcgagg aattcttcag gaggttcaag
     2341 gactcgttgg tcgcgggcgg aatgatcaac cgcagggcga tggggatgat gatgagccgg
     2401 tacgcctgtc tcacggaaag acccgtggcg acggaggcct ccagcagcgt cttggggatg
     2461 gactggatcc cggcccggat gacttcggac atccgggccc cgtggtacac gctcaagccc
     2521 acgacggcgg accagaactc cgggccgtgg tgattcagcc agtcctgggc ggcacgaggg
     2581 agcacctggg gggcgccgaa gtaccagaag aacagccaga ccaggagcgg gacgtttcgg
     2641 aagaactcga cgtagcaggt ggccagcgcc cgcaggggtg caaagggcac ggtccggagc
     2701 gatccggaca gaattccgag aaaggatgcc aagatccagc tcagggcgga gagccggagg
     2761 gttgtccaga ttccctggag gagccactgg ccgtgttctc ccgtccagag cacgctccac
     2821 tggaagtcgt attgcattcc caggaatgcc gtgaggggca tggata
//
LOCUS       DBNGZC010000486         1230 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_139273, whole genome shotgun sequence.
ACCESSION   DBNGZC010000486 DBNGZC010000000
VERSION     DBNGZC010000486.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_139273"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..811
                     /locus_tag="AC16G0_08535"
     CDS             236..811
                     /locus_tag="AC16G0_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099699.1"
                     /translation="MTMHAPQRTRSCRRDQPTATTSQSRGSRQICLPLDPETYQQVWD
                     DARAVRRLVEGLLSRHPELFPEALSRGFTLCGKLPLSRKLPGICLRRVRVLQTDAQGQ
                     AAPRDFFLRPSFVLPYCCGTVDGVANGMLLLSYGVPYHVVAVCCGRNAMAWYRLERAL
                     GRNSVVGTTVRDPRVLPRHLAADEHHAQLRG"
     gene            827..>1230
                     /locus_tag="AC16G0_08540"
     CDS             827..>1230
                     /locus_tag="AC16G0_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099700.1"
                     /translation="MTAGDQCLLGIAVSPTASEVDLTAAYGRFRDEAADVSAAYTPET
                     VNTDGWQATRAAWRALFPTIAVILCFLHGFLKIRDRGRKAFELHRKVWEVYRAISALA
                     FREAMEAFRQWCLARVWSGPIHEALESLWGHT"
BASE COUNT          196 a          389 c          418 g          227 t
ORIGIN      
        1 caagcaacca aaggacattg agtcggcttc tgacgggtcg gggcggaaac tggcggaagg
       61 actgtgggaa tagaatgggt ggggcatcga ggagtgtgac gagaggaaag tctccatgcg
      121 gagaggaact cgcctgaccc tacctctttc tgcgtgtgca taagagccgg gttgccggga
      181 ggcagtccat cctctgaaat cctgctggtg aaatcgttcc cggaacgaag gcaggatgac
      241 gatgcacgcc ccccagcgaa cccggtcgtg ccggcgggat cagcccaccg ccacgacctc
      301 ccagtctcga ggctcacgcc agatctgtct acccctggac cccgagactt atcaacaggt
      361 ctgggatgat gcccgagccg tacgccgcct cgtcgaaggc ctgctctccc ggcatccaga
      421 gctgttcccc gaggccctgt ctcggggatt caccttgtgc ggcaagctgc cgctgtcccg
      481 gaaactcccg ggcatctgtc tccgccgggt tcgggtcctg cagaccgatg cgcagggcca
      541 agccgcgccc cgggatttct tcctcaggcc cagcttcgtc ttgccctact gctgcggcac
      601 cgtcgacggc gtcgccaacg ggatgctgtt gctgagctat ggcgtgcctt accatgtcgt
      661 cgctgtctgc tgcggtcgca atgccatggc ctggtatcgc ctggaacggg cgctggggcg
      721 caacagcgtg gtgggtacga cggtgcgtga ccctcgggtc ttgcctcgac acctggccgc
      781 ggacgagcat catgctcagc tccggggtta gagcgcctac gttgcgatga ccgccggcga
      841 ccagtgcctg ttggggatcg cggtgagtcc cacggccagc gaggtggacc tgaccgccgc
      901 ctacggccgg ttccgggacg aggcggcgga tgtgtccgcc gcgtacaccc ccgagacggt
      961 caacaccgac ggctggcagg cgacccgggc cgcctggcgg gcgttgttcc cgacgatcgc
     1021 cgtgatcctg tgcttcctgc acgggttctt gaagatccgc gaccgtggcc gcaaggcctt
     1081 cgagcttcat cgcaaggtct gggaggtgta ccgggcgatc agtgcgctgg ccttccgcga
     1141 ggcgatggag gcatttcgtc agtggtgcct ggcacgggtg tggtcggggc cgattcacga
     1201 ggcgttggag tcgctctggg gtcacaccga
//
LOCUS       DBNGZC010000487         5751 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1215205, whole genome shotgun sequence.
ACCESSION   DBNGZC010000487 DBNGZC010000000
VERSION     DBNGZC010000487.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5751)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5751
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1215205"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="AC16G0_08545"
     CDS             <1..287
                     /locus_tag="AC16G0_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099701.1"
                     /translation="NAGRRMRELNEAFEVLSDPARRRAYDRQRSVGSRQASGPLPTSF
                     QVSLKSLLPYVGFAILVVLSLRILPLLLRPPVLAALVVAVCVYFFLRRLR"
     gene            complement(300..1145)
                     /locus_tag="AC16G0_08550"
     CDS             complement(300..1145)
                     /locus_tag="AC16G0_08550"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5099702.1"
                     /translation="MIPPFRTLVWHFPLLLAGILGTGMFLGLEKYVIYVPERAIEMTP
                     QSEGLAYEDVWFQAKDGVKLHGWLVPAPGARFTLVWFHGNAGNISHRVDNIKYLHRSL
                     GRPLLPNIFIFDYRGYGQSAGSLSDLSEEATYRDAEGALAYLRGRQDLAQTKAVYFGR
                     SLGSAIAVEMGRQHTPVGMILETPLTSISDMARATLPFLPVGWLLRTKYDSLSKMKEI
                     RVPLLVLHGDRDDVVPYEQGRRLFEAANQPKVFYTIRGARHNDTYIVGGQAYFDAWAR
                     FLGGL"
     gene            complement(1260..2498)
                     /locus_tag="AC16G0_08555"
     CDS             complement(1260..2498)
                     /locus_tag="AC16G0_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR5099703.1"
                     /translation="MNVLLVSANTERMNLPTLPYGLAFVAAAARSAGHRVAMLDLLGE
                     TDPQAAIARAVQTLCPDVIGISVRNIDDQFMERPRFLLEHVAGVVAACRASSRAPLVL
                     GGAGYSIFPTAVLAYLGADFGVCGEGELVFPELLSRLQRGENPSDLPGVYAGGWSSKI
                     PRAFVPDLEVLPSPAEDPWPTANPTDPDLWIPVQSRRGCPLGCSYCSTASIQGRIIRA
                     RPPALVVAEVARLARAGFRRFYFVDNTFNRPPDYALELCRRVTEQGLDIAWRCIIYPH
                     AVREELVRAMAAAGCVEVGLGFESGSPRILQAMNKRFLPAEVREISGRLAAHGIRRMG
                     FLLLGGPKETQASIEESLGFAHSLHLDQLKVAVGIRIYPHTPLARRAIQEGMLAPEDD
                     LLTPRFYLTPGLDYPAAQVA"
     gene            complement(2875..3096)
                     /locus_tag="AC16G0_08560"
     CDS             complement(2875..3096)
                     /locus_tag="AC16G0_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099704.1"
                     /translation="MATRVKFFFGIRDLEGEINTWLSEQSAEVVPLHVSMCAYGSGAQ
                     ADAVVSLWYKSDSTGTRGEADLPSEQRLI"
     gene            3839..4180
                     /locus_tag="AC16G0_08565"
     CDS             3839..4180
                     /locus_tag="AC16G0_08565"
                     /inference="COORDINATES: protein motif:HMM:NF018744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1344 domain-containing protein"
                     /protein_id="HIR5099705.1"
                     /translation="MRQVLAALVTLWLVGSPAWAQMKPPMQNPPGPLAPPTPAEPRRL
                     QPTAVQGKIKSLDPSSNTMTLEDGTTLTIPESVAATGLKEGTRVIVTYNADNGQKVVT
                     SVIQVRESSRS"
     gene            complement(4311..5426)
                     /locus_tag="AC16G0_08570"
     CDS             complement(4311..5426)
                     /locus_tag="AC16G0_08570"
                     /EC_number="2.7.7.-"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR5099706.1"
                     /translation="MRTPARVLVVDDNPENLDIFQARLARHGYEILTAKDGEEALATA
                     RATGPDLILLDIMMPKLDGIEVCRRLRADPSFPFVPIILVTAKADSKDIVAGLEAGAD
                     EYLTKPVDQAALVARVKSMLRIKQLHDTVQKQTAELSNWNRELEGRVQEQVAQLERLG
                     RLKRFFSPQLAELIVAGDAEDPLKSHRREVTVAFLDLRGFTAFADTSEPEEVMGVLRE
                     YPAEMGRLILAHEGTLERFTGDGMMIFFNDPVPVPNPGGRAIRMALAMRDRVRELIDR
                     WRKLGYELDWGMGLAQGYATIGAIGFEGRWDYGAIGTVTNLAARLCGEAGPGQILVPR
                     RFLGTVEGLVEAEPVGELSLKGFHRPVTAYNILGLKG"
     gene            complement(5522..>5751)
                     /locus_tag="AC16G0_08575"
     CDS             complement(5522..>5751)
                     /locus_tag="AC16G0_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003562240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5099707.1"
                     /translation="DLILMDIQLPGLDGYEATRRIKATPALRHIPIIAVTSYALSGDD
                     VKARVAGCDAYVTKPYSPRALLAKVREYLPQ"
BASE COUNT         1077 a         1825 c         1820 g         1029 t
ORIGIN      
        1 cgaatgcagg tcgccggatg cgagaactga acgaggcttt tgaggtcctc agcgatcctg
       61 cgcgccgtcg ggcctacgac cggcagcggt ctgtcggcag ccgccaggct tcaggccccc
      121 tcccgacaag cttccaggtc agcctgaagt cgcttctgcc ttacgtcggt ttcgccatcc
      181 tcgtggtctt gagcctccgc atcctcccgc tgctcctgcg ccccccggtc ctggccgccc
      241 tcgtggttgc cgtttgcgtg tacttcttcc tgcgccgtct ccgctgaagg aggcgctggc
      301 tacaggccgc ccaggaagcg agcccaggcg tcgaagtacg cctggccccc cacgatatac
      361 gtgtcgttgt ggcgcgctcc acgaatcgta tagaacactt tgggctggtt cgccgcctcg
      421 aagagacggc gcccctgctc atagggcacc acgtcatccc gatcgccgtg cagcacgagc
      481 aatggcaccc ggatctcctt catcttgctc aacgagtcgt acttggtccg aagcaaccac
      541 cccaccggaa ggaaaggcaa ggttgcccgc gccatgtcac tgatggaggt gagaggcgtc
      601 tccaggatca tgccgaccgg cgtatgctgt cgtcccatct cgacggcgat ggccgatccc
      661 agcgagcgtc cgaaatagac ggccttcgtc tgggccagat cctggcgtcc tcgaaggtag
      721 gccagggccc cctcggcgtc gcggtaggtt gcctcctccg agagatcgga gaggctgccg
      781 gcactctgcc cgtagccgcg atagtcgaag atgaagatgt tgggaaggag gggccggccc
      841 agggagcggt gcaggtattt gatgttgtcc acccggtggc tgatgttgcc ggcgttcccg
      901 tggaaccaca cgagcgtgaa gcgcgcgccc ggcgcaggga ccagccagcc gtgcagtttc
      961 acgccatcct tcgcctggaa ccagacgtcc tcgtacgcca gcccctcgct ttgcggggtc
     1021 atctcgatgg cccgttcagg gacgtaaatg acgtatttct caagacccag gaacataccg
     1081 gtccccagaa tgccggccaa gagcagcggg aagtgccaga ccagtgtccg aaacgggggg
     1141 atcacggatt cccctcaggg tacgccaggg ctacaagcag tcggcccagg cattctggat
     1201 gcccaagggg cgggtggaag ccgacgggtc ggccatggca ccattttcag tcgcgagcct
     1261 cacgccactt gcgcggcggg gtaatccagg ccgggggtca ggtagaaacg tggcgtgaga
     1321 aggtcgtcct cgggtgcgag catgccctcc tggatggctc ttcgcgccaa gggcgtgtgg
     1381 gggtagatgc gaattcccac cgcgaccttc agttgatcca ggtggaggga gtgggcaaag
     1441 ccgaggctct cctcaatcga tgcctgcgtc tccttgggac ctcccagcag gagaaacccc
     1501 atccggcgaa tgccatgcgc agccaaccgg ccggagatct cgcggacctc ggcggggagg
     1561 aatcgcttgt tcatcgcctg cagaatccgc ggggagccgc tctcaaaccc cagaccaacc
     1621 tcgacacagc cggccgcggc catagcccgg acgagctctt cgcgtactgc gtgcgggtag
     1681 atgatgcagc gccaggcaat atccagcccc tgctccgtca cccgccgaca caactccagg
     1741 gcgtagtccg gtgggcggtt gaaagtgttg tccacaaagt aaaaccgccg gaaccccgcg
     1801 cgggcgaggc gggcgacctc ggcgaccacg agcgccgggg ggcgggcgcg gatgatccgc
     1861 ccttggatgc tggccgtgga acagtaggag caccccagag gacagccgcg ccggctttgc
     1921 acggggatcc agaggtcggg atcggtggga ttcgccgtgg gccacgggtc ctctgcgggc
     1981 gagggcagga cctctagatc cggcacaaag gcccggggga tcttgctgga ccacccgcct
     2041 gcgtacactc cggggaggtc actagggttc tcaccccgct gaagccgcga caaaagctcc
     2101 gggaagacca actccccctc gccacacacg ccgaagtcgg ccccgaggta agccagcacc
     2161 gcggtgggga agatactgta gcccgcgccg ccgagcacca gcggtgcccg ggaagaggcc
     2221 cggcatgccg ccacgacacc agcgacgtgc tccaggagga agcggggtcg ctccatgaac
     2281 tggtcatcga tgttccgcac ggaaatgcca atgacatccg ggcagagggt ctggactgcg
     2341 cgagctatgg cagcttgggg atcggtctca ccgaggaggt cgagcatcgc cacccggtgg
     2401 ccggcggaac gggctgctgc ggctacaaag gccaagccgt aggggagggt ggggaggttc
     2461 atccgctccg tgttggccga aacgagcagg acgttcatcg gctgcctcga gcccttccca
     2521 tgcgcgcacc tcgggcacga ccgaccctgt gccgcaacgg atctgaggca ggaatgctga
     2581 gactccgccg cgcgatgaga ttccgcacag ctatccccta tcgtgcggaa gaggttaccg
     2641 cgaaaggacc aaaccggccc gatacacgcg gtagctaggg aggagagcgg gcgctcccgc
     2701 gaatatggga taagcgccga gaagctcgtc tgggagcgta tcgctcagtc ctcgcagtgt
     2761 caagtgaacc tcgctcggac gcactggcca gcataccatc gtatggcgat atgcggcaca
     2821 cacatagagg tctggcagga tgaagcggat atgcgagttc cgcggacggc tcgatcatat
     2881 gagtcgctgt tcagacggga gatctgcttc accccgggtg cccgtggagt cgctcttgta
     2941 ccagagcgag acgacggcgt ccgcctgcgc tcctgagcca taggcacaca ttgaaacatg
     3001 gagcgggact acctcggcgg actgctcgga aagccaggtg ttgatctccc cttccaggtc
     3061 acgaatacca aaaaagaact ttacccgagt ggccatgtct cctcctctct tctggtcgct
     3121 cctcgacatc gaccgtacgc cacagcgccg cgcctgcgcg ccgggtaccc attcggcgca
     3181 tgggcaccca tccactggtc cttggcagat cccagcagga cgctagacat ccccgcgtct
     3241 tgcgcatgat ctcacgaact caaagcttga gccagcacca cttgatcgga aattagaccc
     3301 cccgacttca aggccggggg actggtgggc acgccatatt cactgcgggt caggcggggt
     3361 gttcgatggt ggcacaacgg tcagccaacc accggggcat tccttgatgt atgaatggta
     3421 gctatgcgga ttctagcagt agtacaaggt cgaacttctc gaagggattg gagggagttc
     3481 ttgcggacga cgacgattgg gtgaacgcat gtaagaatta caggtctcca gaagatcaga
     3541 agttctcagt ttttcagaga catgtatcag gttgtggtaa ttcttactgg gtgtgattgg
     3601 agggtcaggc gctttcaatt ccccagcgcc gaacgacatt ttttgacctg gcatccagtg
     3661 atgcctgttc ctatcggtac ttcacggact atttcaacac gcctcgtcaa ctggaacgca
     3721 atttgctgca tcgataaacc gcctagtagg ccagcctctc accctatcct cttgggaaga
     3781 acggatgggg tagaaagggt tgggaggcca agtcttgtgg ccaaaggagg tacaacggat
     3841 gcggcaagta ttggcagccc tggtcactct ctggctggtc ggatcccctg cctgggcgca
     3901 aatgaagcca ccgatgcaga atccgcccgg ccccttggcg ccgccgactc cagcggaacc
     3961 caggagactt caaccgacgg cggtccaggg caagatcaag agtctggacc catcgagtaa
     4021 caccatgaca ctcgaggacg ggaccaccct cacgatcccc gagtccgtcg cggcgactgg
     4081 cctgaaagag ggaacgaggg tcatcgtgac gtacaacgcc gataacgggc aaaaggtggt
     4141 gacttcggtc atccaggtac gggagtcctc caggtcctag cccagatatc cgcccatcga
     4201 aggcctagca gcctgagatg tcgatcttgg gccaccatcc ttctcagtca actcttttga
     4261 tggaaaccgt tgccaagggg gagcccaatc ccattcgcct ggttatctgc ttatcccttc
     4321 aggcccagga tgttgtaggc ggtgaccggg cggtgaaagc ctttgaggga gagttcgccc
     4381 acaggctcgg cctccaccag gccctccacg gtcccgagga agcggcgagg caccaggatc
     4441 tgtcctggcc ccgcctcgcc gcagaggcgg gctgcgaggt tcgtgacggt gccgatggcc
     4501 ccgtagtccc atcgaccctc gaagccaatg gcaccgatgg tggcgtatcc ctgcgccagc
     4561 cccatgcccc agtcaagctc atagcccagt ttccgccacc ggtcaatcag ttcccgtacc
     4621 cggtcccgca tcgccagggc catccggatc gctcgccctc ccgggttggg taccggcacc
     4681 gggtcgttga agaaaatcat cattccgtcg ccggtgaacc gctccagtgt cccctcgtgg
     4741 gcgaggatga gcctgcccat ctcggcgggg tactcgcgga ggacgcccat gacctcctcc
     4801 ggctccgagg tatcggcaaa ggcggtgaag ccccgcaggt ccaggaaggc gaccgtcacc
     4861 tctcgccggt ggcttttcag gggatcctcg gcatcgcccg ccacgatcaa ctcggcaagc
     4921 tggggcgaaa agaaccgttt gagccggccc agccgctcga gctgcgccac ctgctcctgg
     4981 acgcgcccct ccagctcccg gttccagttc gatagctccg ccgtctgctt ctggacggta
     5041 tcgtggagct gcttgatgcg cagcatggac ttcacccggg ccacgagcgc agcctggtcc
     5101 acgggcttcg tgaggtactc gtccgcgccc gcctccagac cggccacgat gtccttcgaa
     5161 tcggccttgg ccgtcaccag gatgatcggc acaaagggga aagaagggtc cgccctgagc
     5221 cgccggcaga cctcgatgcc gtccagcttc ggcatcatga tgtccaggag gatcaggtcc
     5281 ggccctgttg ctcgggccgt cgccagagcc tcctcgccgt ccttcgccgt caggatctca
     5341 tagccgtggc gggccaggcg ggcttggaag atgtccaggt tctccgggtt atcgtccaca
     5401 acgagaacgc gcgctggtgt ccgcatgctc tgccctcagg attctctaag tgctttcagc
     5461 tatcagcggt cagccctggt catccggcat tcgaccttgg tcattcttca ttcgccattg
     5521 gtcattgcgg caggtactcc cgcaccttgg cgagcagggc gcgggggctg tagggcttgg
     5581 tgacgtaggc gtcgcagccg gccacgcggg ccttcacgtc gtcgccgctc agggcgtagg
     5641 aggtcacggc gatgatcggg atgtgccgga gggcgggggt ggccttgatc cggcgggtgg
     5701 cctcgtagcc gtcgaggccg gggagctgga tgtccatgag gatgaggtcg g
//
LOCUS       DBNGZC010000488         6225 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1215212, whole genome shotgun sequence.
ACCESSION   DBNGZC010000488 DBNGZC010000000
VERSION     DBNGZC010000488.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1215212"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="AC16G0_08580"
     CDS             complement(<1..1208)
                     /locus_tag="AC16G0_08580"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR5099708.1"
                     /translation="MTQARLLVVDDDPVSCEAVAEALRAEGYEVEMTRGGRAALALAK
                     ERLFDVVVSDIRMPDLDGLGLLHGLHAVSPDISVILMTAFGTVEAALAAIKEGAYDYV
                     GKPLRLDELLLTIRRALEQRRLVRENQRFRQTLQERYHLDNIVGVSPRMIEVFKLIAR
                     VAPTRSTVLITGESGTGKEVVAHALHWNGPRAAGPFITIDCGGLAEGLLESELFGHVR
                     GAFTGALTTRRGLFEAGAGGTVLLDEIGEVGPNIQAKLLRVLELQEVRPIGSNEPIRI
                     DVRLIAATNKDLSAEVREGRFREDLFYRLNVVRIELPPLRERREDIPVLAHHFLRKYA
                     ARNGKAVDAIAPEAMALLEAHPWPGNVRELENAIERAIAVSGHSVLLPDDLPLHLASP
                     HTQSAVAPGDL"
     gene            complement(1205..2770)
                     /locus_tag="AC16G0_08585"
     CDS             complement(1205..2770)
                     /locus_tag="AC16G0_08585"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5099709.1"
                     /translation="MRSWSLRWKIAALVVGLTAGSSLAASAVHGWLAFQALKEEVRGR
                     AAGVVSDITFGITTPEELANRDLLALEIRNIMAARPTLRWLDIYARGPTGLSPIASSR
                     EPLPLRPPDLVTQALAQGRTVTAAGMGGGDEIWQAAAPVRFGEDAAGVVALAISLEGA
                     NRLAVTLGQQLLLVLVVSGLTIVTSITLFMERSINRPIRELLHTMAAVERGDLTAEPQ
                     YDRRDEMGQLADGLAHMLRRIRESQEENALLLARINHFNQDLQLRVAEATRELADRNQ
                     ALRRANELLFDLQRQLGRAQRLATLGQLAATIAHEIGTPLNSIAVHLQLLARSPGLSQ
                     QDRQRLVTIDAQIQRLVATVQARLAATRGEARRLQPTDLHQLVRRIIDLMAPILAAKG
                     IGLHPGVDGPQLMIQADEHQIQQVVLNLLTNAVDAMPTGGSLAVEAGLSDGMACLTIA
                     DSGPGVPPEAREQIFEPFFTTKERGGGTGLGLTICRQIVEEHGGSIQMNDTPGGGATF
                     VIRLPLAAEEGQE"
     gene            2988..3617
                     /gene="rdgB"
                     /locus_tag="AC16G0_08590"
     CDS             2988..3617
                     /gene="rdgB"
                     /locus_tag="AC16G0_08590"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HIR5099710.1"
                     /translation="MTNIPPIHRITLVIATRNPGKLNEIVALLQGLDALLVPLDRVGP
                     CVIPPEGGESCQVNARRKAEAVAGATGFLALADDSGLEVDALGGQPGVLSARFGGEQA
                     TDADRNRRLLENLRSIPIERRTARFRCVVAIAEPHGQVHLAEGTCEGRIALAPRGTNG
                     FGYDPVFEIPSLGQTLAEVPSAVKNRMSHRAQALAKAREILREVLADHD"
     gene            3667..3743
                     /locus_tag="AC16G0_08595"
     tRNA            3667..3743
                     /locus_tag="AC16G0_08595"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3701..3703,aa:Pro,seq:tgg)
     gene            3791..3868
                     /locus_tag="AC16G0_08600"
     tRNA            3791..3868
                     /locus_tag="AC16G0_08600"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3826..3828,aa:Arg,seq:ccg)
     gene            3953..4029
                     /locus_tag="AC16G0_08605"
     tRNA            3953..4029
                     /locus_tag="AC16G0_08605"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3987..3989,aa:His,seq:gtg)
     gene            4049..4125
                     /locus_tag="AC16G0_08610"
     tRNA            4049..4125
                     /locus_tag="AC16G0_08610"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4083..4085,aa:Arg,seq:tcg)
     gene            4213..4289
                     /locus_tag="AC16G0_08615"
     tRNA            4213..4289
                     /locus_tag="AC16G0_08615"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4247..4249,aa:Arg,seq:tct)
     gene            4357..4432
                     /locus_tag="AC16G0_08620"
     tRNA            4357..4432
                     /locus_tag="AC16G0_08620"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4390..4392,aa:Lys,seq:ctt)
     gene            5248..6015
                     /locus_tag="AC16G0_08625"
     CDS             5248..6015
                     /locus_tag="AC16G0_08625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5099711.1"
                     /translation="MDTGKLEGQAAIVFGASSGMGRATALAFAAEGSKVVVSARRTAE
                     LQTLAAEITKSGREAVALAAEVSRRPDVEAVVQATVERFGRVDVLVNAAGVNTRNRQL
                     AHLDQAGWDRIIGINLTGGFHCTQAVLPQMRAQKGGLVIHVSSISGLWGDFSGAAYQA
                     SKHGLTGLANATMMEERLNGIRVTVICPGLCDTPLLNNRPVPPTQEERDRMMRPEDIA
                     QACVFAASLPPRTYVSELVLMPGALQCNGHGVGSSGV"
BASE COUNT         1099 a         1904 c         2081 g         1141 t
ORIGIN      
        1 aggtcgccag gcgcgacggc agactgcgtg tgcggcgagg caaggtggag cggcagatca
       61 tcgggcaaca ataccgagtg gccgcttacc gcgatcgccc gctcgatggc gttctcgagt
      121 tctcggacgt ttcctggcca cggatgggcc tccaggagag ccatcgcttc aggggcgatc
      181 gcgtccacgg ccttcccgtt cctcgcggca tacttccgta ggaaatgatg cgccaggacg
      241 gggatgtcct cgcgacgttc ccggaggggt ggaagctcga tgcgcacgac attcaaccgg
      301 tagaacaggt cctctcggaa gcgaccctcg cgaacctcgg cagacagatc cttgttcgtg
      361 gcggcgatca ggcgaacgtc gatgcggatc ggctcgttgg atccaatcgg tcggacttcc
      421 tggagctcaa ggacccgaag gagcttggcc tgaatattcg ggccgacctc accgatctcg
      481 tccagcaaga cggtccctcc ggcaccagcc tcgaacagcc cgcgtcgggt cgtcagcgcg
      541 ccggtgaagg cgccgcggac gtgaccgaac aactcgctct ccaggagacc ttcggccagg
      601 ccgccacaat cgatagtgat gaatgggccg gcggcgcgag gaccgttcca atgcagggcg
      661 tgcgcgacca cctcctttcc cgtcccgctc tccccggtaa tcaggaccgt actccgggtc
      721 ggagctaccc gcgcgatgag cttgaagact tcgatcatgc gcgggctgac gccgacgata
      781 ttgtcgaggt gatatcgctc ctggagcgtc tggcggaatc gctgattctc acggacgagc
      841 cggcgttgtt cgagcgcacg gcgaatcgtg aggagcagct catccaggcg gagaggcttt
      901 cccacatagt cgtaggcacc ctccttgatg gccgccaggg ccgcctcgac ggtgccgaag
      961 gcggtcatca ggatcacgct gatgtccggg ctgaccgcgt ggagcccgtg taagagcccg
     1021 aggccgtcca ggtcaggcat gcggatgtcc gagaccacca cgtcgaagag ccgctctttc
     1081 gccagcgcca aggcggctcg tccgccccgc gtcatctcaa cctcgtaccc ctcggcgcgg
     1141 agtgcttcgg ccacggcctc gcaggacacg gggtcgtcat ccacgaccag caggcgggcc
     1201 tgggtcattc ctgcccctcc tcggcggcga gcgggaggcg gataacgaag gtcgccccgc
     1261 cacccggcgt atcgttcatc tggatgctgc ccccgtgctc ctccacgatc tggcggcaga
     1321 tggtgagacc cagcccggtt ccaccgcctc tttccttcgt cgtgaaaaag ggttcgaaga
     1381 tctgttcacg ggcctctgga gggacaccgg ggccggagtc agcgatcgtc aggcaggcca
     1441 tgccgtccga cagaccggcc tcgacagcga gcgagccgcc agtcggcatg gcgtccacgg
     1501 cgttggtcag caaattgagc acgacctgtt ggatctgatg ttcgtcggcc tggatcatga
     1561 gctgtgggcc gtctacgcca gggtgtagcc caatgccctt ggctgcgagg atgggagcca
     1621 tcaggtcaat tattctccgg acgagttggt gcagatcggt cggctgcaga cggcgggcct
     1681 caccacgcgt ggcggccagg cgcgcctgaa cggtggcgac cagccgctgg atctgcgcgt
     1741 cgatggtgac gagacgctgg cgatcctgct ggctgagacc agggcttcgt gccaggagct
     1801 ggagatggac ggcgatggag ttgagcggcg tgccgatctc gtgggcgatg gtagcggcga
     1861 gctgtcccag ggtggccaat cgttgtgccc ggccgagctg ccgctggagg tcgaagagca
     1921 gttcattggc ccggcggagg gcctggttcc ggtcggcgag ctcgcgtgtg gcctcggcca
     1981 cccggagctg gaggtcctgg ttgaagtggt taatgcgcgc gagcagaagg gcgttctcct
     2041 cctggctctc ccgaatccgg cgcagcatgt gcgccagtcc gtcggcgagc tgccccatct
     2101 cgtcccgccg atcgtactgg ggttcggcag tgaggtcgcc ccgctcgacc gcagccatgg
     2161 tgtgcaacag ctcgcggatc ggccggttga tgctccgctc catgaagagg gtgatgctcg
     2221 tcacgatcgt gaggccgctc acgacgagca ccaggagcag ttgctggccc agggtgacgg
     2281 ccaggcgatt ggccccctcc aacgagatcg ccagggcaac gacaccggcc gcgtcctcgc
     2341 cgaaacggac gggggcggcg gcctgccaga tctcatcgcc cccgcccatc ccggcggcag
     2401 tcaccgttcg tccctgggcg agggcctggg tcacaaggtc gggcggccgc agtggcaggg
     2461 gctcgcgact ggaggcgatt ggcgagagac cggtcggtcc tcgggcgtag atatccagcc
     2521 accggagcgt cggtctggcc gccatgatgt tccggatctc cagggccaag agatcgcggt
     2581 tggccaattc ttccggtgtc gtgatgccga aggtgatgtc tgataccacg cccgcagccc
     2641 ggccccttac ctcttccttc aacgcctgga aggcgagcca cccgtggacg gcgctcgcgg
     2701 ccaggctgct cccggcggtg aggcccacga ccagggcagc gatcttccag cggagcgacc
     2761 aggatctcat tcgcgatgac ctcgcaggcc ttcaggatgc aggtatctta gcctaaaggc
     2821 ccctccgagt catctccgca tccgatctta ccctttatct ggcacccaag ggatggacca
     2881 aattttggat tgtgcgggag tagcgtttca tgctatcaga cggcgtggtg tgtatcccgg
     2941 tggtccttaa gggtccatga tacctgtccg tgtcgggcaa tagttccttg acaaacattc
     3001 cccccatcca tagaatcacg ctggtcattg ccacgaggaa tcccggcaaa ctgaacgaga
     3061 tcgtcgcgtt gttgcagggc ctggatgccc tgctcgtgcc gctcgaccgg gtggggccat
     3121 gcgtgatacc cccggagggc ggcgagtcgt gtcaggtgaa cgctcggcga aaggccgagg
     3181 cagttgccgg cgccactggc tttctcgcct tggcggatga ctccgggctg gaagtggatg
     3241 cgctgggggg acagcccggt gtgctgtccg ccagattcgg cggagaacag gcgaccgacg
     3301 cagatcggaa ccggcgactc cttgaaaacc tccgaagcat cccgatcgaa cggcggacgg
     3361 ctcgcttccg ttgtgtggtt gccatagccg agccgcacgg tcaggtgcac ttggcggaag
     3421 ggacctgcga ggggcgtatt gcgctagcac cgcgcgggac gaacggcttc ggttacgatc
     3481 ctgtatttga gatcccgtcc ctggggcaga ctctcgccga ggttccttcg gcggtgaaga
     3541 accgcatgag tcatcgcgcc caggcgctgg cgaaagcgcg ggagatcctc cgggaggtcc
     3601 tggcggatca cgactaattc aggaaaggcc agacgcgatt gcgcggtgca gccatcaaga
     3661 tcgtctcggg gcgtggcgca gcctggtagc gcacctgctt tgggagcagg gagtcggagg
     3721 ttcaaatcct ctcgccccga ccatcggcgg gagcaatgtg gcggtagcgc tgggactgat
     3781 gaagggagat gcgcctgtag ctcagcccgg atagagcaac ggcctccgga gccgtgtgtc
     3841 gcgtgttcaa atcacgccag gcgcgccagt ttggattggg gactgcagat cgaagctggc
     3901 agatctttcc gtccagaatc tgccatctga catctgtcat ggctgtggca tggtgagcgt
     3961 agctcaactg gtcagagcac tggactgtgg ctccaggggt tgagggttcg agtccctccg
     4021 ttcaccccaa tcagcttggg ccgaggaggc gcccgtagct cagtcggata gagcggctgg
     4081 cttcgaacca gcaggtcggg agttcaatcc tctccgggcg cgccaggata tgagtggtcg
     4141 atgaccaaac gccgactgac cgacggatgg aggtaaggca ttggtcggcg ggtgttggtc
     4201 atgccaacga gggcgcctgt agctcagacg gacagagcaa ctgccttcta agcagtgggt
     4261 cgggcgttcg agtcgcccca ggcgcaccag ccagaacagg cttgagatcg cgcccacgac
     4321 tcccggccag agggagtttg gggcgtgatg cagattgggc cgctagctca gatggtagag
     4381 cagctgactc ttaatcagcg ggtcgtaggt tcgagtccta cgcggcccac catactcagg
     4441 tagtttcgac acccaatctc gtccttggtc tgctcctgcc agcattttgc tttcactcac
     4501 cctgtgatcc gggatcatcc tgtcctctca tcgcctcgct cagcgtttcc tcgacatggg
     4561 tgtagaaagg tgtcttcctc tgtgcgcaac actacgcgct gacctgagga ggcacgaatg
     4621 gtcggaggat ctgacccctg cgggaaggcg gcagtcgtca ctgggtcagg acggcggaga
     4681 gtgacccaga tgctggaagc actattcatc atgtaggtgc acttgggaat cacagggaat
     4741 gttgttgcgc ccggttggtc ccgaacgtcc agaccgagac ccttccatct gcttgctacg
     4801 ctggacttct tcatatagga tgacattcgg cgtgatttct ggtagcgccc gggccagcct
     4861 tggaggtgtg agaaactatg cggagtttcc cctgggcgca cctgctccaa gccgacttcg
     4921 ccctctcctg ggattccgcg accttcatct tatgcgctcg aaccgagcca acccttgcga
     4981 tcgaaaaatc tcttgctttg aaagcagctg gcgatgtgtc gcgagcgacg tgcaaaggcc
     5041 acccgggcca cggatctaat tcgggcggca ttggcactcc tggcgctcta gttagcccgg
     5101 tgagacgcac tgacctacca ttctggcgct ggaattcacg cccgcgtgga cacgtccggc
     5161 gtcccgccga cctccgttcg acgcgccgga tagcgcccag actgccgggt tgaaggaaat
     5221 gactggcgga cagacgagga gaatcgaatg gataccggta agttggaggg tcaggcagcc
     5281 atcgtttttg gggcgagcag cggtatgggc cgagctaccg ccctcgcctt cgcggcagag
     5341 gggtcaaagg tcgtggtgtc tgcccgccga acagcagagc tccagacact cgccgcggag
     5401 atcacgaagt ccggccggga ggccgtcgcc ctcgccgccg aggtgagccg gcgtcccgat
     5461 gtcgaggcgg tcgtgcaggc gacggtcgaa cggtttggta gggtggatgt cctggtgaat
     5521 gccgccggcg tcaatacgcg caaccgccaa ctggcgcacc tggaccaggc cggctgggat
     5581 cggatcatcg gcataaacct gaccggcgga ttccactgca ctcaggcggt cttgccacag
     5641 atgcgcgcgc agaagggcgg actcgtcatc cacgtctcgt ccatatcagg attgtgggga
     5701 gatttttccg gcgcggcgta tcaggccagc aagcacggcc tcacgggact ggcgaacgcg
     5761 acgatgatgg aggagcgact caacggcatc cgggtgacgg tcatctgccc gggtctctgc
     5821 gacacccccc tcctgaacaa ccggccggtg ccgccgaccc aggaagagcg cgaccgaatg
     5881 atgaggccgg aagacatcgc ccaggcgtgc gtcttcgcgg cgagcctgcc gccgcggact
     5941 tacgtctcgg agctcgttct gatgcccggc gcgctccagt gcaacgggca tggggtggga
     6001 tcgagcgggg tgtgagggat gaaagcaggg ctccattctg acccggcccc gatatgcgtc
     6061 ggcaccgatt agtccgagga caggatcgtt tccgcgaagg gggagtccat gaaatcggtc
     6121 cgtgtgcatg aactgggcgg ggtcgaggtc ttgcgttacg aggacattcc cattcctgct
     6181 cccggtgccg gacaggcgct cgtcaaggtc gaagcgatcg ggctg
//
LOCUS       DBNGZC010000489         2636 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2485567, whole genome shotgun sequence.
ACCESSION   DBNGZC010000489 DBNGZC010000000
VERSION     DBNGZC010000489.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2485567"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="AC16G0_08630"
     CDS             <1..251
                     /locus_tag="AC16G0_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099712.1"
                     /translation="RAEVTEESAAEEPSAGVSPERIQVQLINGERLTGTVCIEGPPGK
                     RRLSDFLNTQPAFLPLHGRDRLHLLHKRFITRIVPQNS"
     gene            454..1707
                     /locus_tag="AC16G0_08635"
     CDS             454..1707
                     /locus_tag="AC16G0_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099713.1"
                     /translation="MQKLPSFVSVIVVTLALSFGSAWAGEKVAEVKLGSVMSLSGGAA
                     NQGVQTKTGAIIAADEINAKGGIPALGGAKIKLIFGDSQSKPDVGASETERIIQRENV
                     PIMFGAYQSAVTTVTTEVAERYKTPWLVTGSVADIITQRGFKYTFRPNNKAVYDAKEQ
                     LEAIKTLTQETGTGPKSIGILVEGSDWGKSHATNVRKLAKDMGYEIVLDEAYSPGTVD
                     FSAQILKIRSRKPDAMVIAMYTPDHIVFTKQAYEGKLDLPFGLHSVGAGSEDPNFYKA
                     VPVQAVDLMFVQEDFQIDITEVAPWTQAVDKKFREALGYELTSYGAQGYSAIYTIYDV
                     LQRAGSLDKEKIRDAFTKTNITSGPALIMGWQKIAFDQDGQNVDAHGVISQNFKGKRT
                     TIWPVANRPKGVKPVWPIPAWKDRQ"
     gene            1778..>2636
                     /locus_tag="AC16G0_08640"
     CDS             1778..>2636
                     /locus_tag="AC16G0_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5099714.1"
                     /translation="MDPIILGIAVVNGVIVGGLYGAIALGLSLIFGVMRVINFAHGTF
                     LMLGLFIPFWLWQLFGINPYLSVLVAAPGLFALGYLVQATIIAPLFKRERAMVVEPLS
                     VLLLTAGVGIMLDNLALMTVGPDFRGITLEFAGKALWIGSILNINYTRLIAFLVSLAI
                     AIALSLLLARTDLGRAIRSTAQNRDAAALCGINVSRIYNLTFGLGCAILGIVGCVMIP
                     FYLVSPSVGLAFGVRSFIVVVLGGIGSIPGSILGGLILGVVESVTAQFVTATSASIFS
                     FVIFILVLFF"
BASE COUNT          485 a          848 c          822 g          481 t
ORIGIN      
        1 tacgggcgga ggtgacggaa gagtccgccg ccgaagagcc ctcggccggt gtgagtccgg
       61 agcggataca ggtccagctc atcaatggcg agcgactgac cggcacggtg tgcatcgagg
      121 ggcccccagg caagcggcgt ctttccgact tcctgaacac ccagccggcc ttcctgcccc
      181 tgcacgggag ggaccggctc cacctcctgc acaagcgctt catcacccgg atcgtccccc
      241 agaattcgtg agcggaccgg gtccggccgg cagcaggaag tccttgactc tcgccgtcat
      301 tgtggagcac accgacaaag tgtggggcgc tggtcctggg cggtggggac cgatgcgtcc
      361 cagcggaagg gggtggtagt ggcttctgat attacaggag gctgaatcgg atcttccatg
      421 tggacacggt ctacagatcg aggagggata cacatgcaga agctgccttc attcgtctcc
      481 gtaattgtag tcacgctagc cctctccttc ggctccgcct gggccggcga gaaggtggcc
      541 gaggtgaagc tcgggtcggt gatgtcactc tccggtgggg cggcgaacca aggggtccag
      601 acgaagacgg gcgccatcat cgccgcggac gagatcaacg ccaagggcgg gatcccggca
      661 ctgggcggcg ccaagatcaa gctcatcttc ggcgactccc agagcaagcc cgacgtcggg
      721 gcctccgaga ccgagcgcat catccagcga gagaacgtgc cgatcatgtt cggtgcctac
      781 cagagcgctg tgaccaccgt gaccaccgag gtggccgagc ggtacaagac cccctggctg
      841 gtcaccggct ccgtggccga catcatcacc cagcgtggct tcaagtacac cttccggccg
      901 aacaacaaag ccgtctacga cgccaaggag cagctcgaag ccatcaagac cctcacccag
      961 gagacgggca cgggcccgaa aagcatcggc atcctggtag agggatccga ctgggggaag
     1021 tcgcatgcca ccaacgtgcg aaagctcgcc aaggacatgg gctacgagat cgtcctggac
     1081 gaggcctatt ctccggggac cgtggacttc tccgcccaga tcctgaagat tcggtcgagg
     1141 aagcccgacg ccatggtcat cgccatgtac accccggacc acatcgtctt caccaagcag
     1201 gcctacgagg gaaagctgga tctccccttc ggccttcact cggtcggcgc cggctctgag
     1261 gacccgaact tctacaaggc ggtgcccgtc caggcggtcg atctcatgtt tgtccaggaa
     1321 gacttccaga tcgacatcac cgaggtggcc ccctggactc aggcagtgga caagaagttc
     1381 agggaggcgc tgggatacga gctgaccagc tacggggccc agggctattc ggcgatctac
     1441 accatctacg atgtcctgca gcgggccggt tccctcgaca aggaaaagat ccgcgacgcc
     1501 ttcacgaaga ccaacatcac cagcggaccc gccctgatca tggggtggca aaagattgcc
     1561 ttcgatcagg acggccagaa cgtggacgcc catggcgtca tctcgcaaaa cttcaagggc
     1621 aaacggacga ctatctggcc cgtggcgaac cgccccaagg gggtcaagcc tgtttggccg
     1681 attcctgcct ggaaggatcg gcagtagtcc acgcgaacgg gcagagcgag ggggaggagg
     1741 ctctccctcg ctctgcgaat cctgaaaggc acagcgcatg gacccgatca tcctcggcat
     1801 tgccgtggtc aacggcgtca tcgtgggggg gctgtatggg gccatagctc tcgggctcag
     1861 cctgatcttc ggggtgatgc gggtgatcaa tttcgcccac ggcaccttcc tgatgctcgg
     1921 gctgttcatt cccttctggc tctggcagct cttcgggatc aatccgtacc tgtccgtgct
     1981 ggtcgccgcc ccaggtcttt ttgccctggg ctacctcgtc caggccacca tcatcgcccc
     2041 gctgttcaaa cgagagcggg ccatggtcgt ggaacccttg agcgtgctcc tcctcacggc
     2101 cggggtgggg atcatgctgg acaatctggc cctcatgacc gtcgggccgg acttccgggg
     2161 aattacgctg gagttcgcgg gcaaggcgct ctggatcggc agcatcctca acatcaacta
     2221 cacgcgtctg atcgccttcc tggtgagcct cgcgatcgcc atcgccctga gcctcctgct
     2281 cgcccgcacc gacctggggc gggccatccg ctccaccgcc cagaaccgag atgccgccgc
     2341 cctgtgcggg atcaacgtgt cccggatata caacctcacc ttcggcctgg gctgcgccat
     2401 cctggggatc gtcgggtgcg tcatgatccc attttacctg gtgagcccgt cggtggggct
     2461 ggccttcggg gtacggtcgt tcatcgtcgt ggtgctcgga ggcatcggca gcatccccgg
     2521 gtcgatcctg gggggcctca tcctcggggt ggtagagtcg gtgaccgccc agttcgtgac
     2581 cgccacctcg gcgtcgatct tttccttcgt catctttatc ctggtgctgt tcttcc
//
LOCUS       DBNGZC010000490         1449 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1018206, whole genome shotgun sequence.
ACCESSION   DBNGZC010000490 DBNGZC010000000
VERSION     DBNGZC010000490.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1018206"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1098)
                     /locus_tag="AC16G0_08645"
     CDS             complement(94..1098)
                     /locus_tag="AC16G0_08645"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR5099715.1"
                     /translation="MSRSRQFLIVALAVLVAFACALGSAQAQEKKIVAKIADILSPDH
                     PHSLTMKFFADRVKERTKGRMEIQVFPAAQLGQTKDLFMGVQTGSIEMAKMPMSFSGE
                     WIPESKVWDLPYLFNSREELWKVIRSNVGKKFFTDFYPKQGLAGLFWVDDGGRSIYTH
                     KPIRKPEDLKGLKIRVQPSDIQIQAINEMGGIGTPLAFGEVYLALQQKVLDGAENSPA
                     LLWTSKHWEVAKVYSLTEQFWSVSGFIANKKWWDGLPKDIQAEMIAAGPETEKYFLDT
                     YIALENKAIGFLKEKGVQIVTDVDKHTFEKRIQPVYDNFVKKYAFGKDLLDQARAAKK
                     "
BASE COUNT          264 a          464 c          426 g          295 t
ORIGIN      
        1 agcttgccca cgacccgcct ccacgcacag acgataacgg gggcggtgcg ccacctggca
       61 ccgcccccgc aagtgaaccg cgtcccgacg cgtctacttc ttcgcggcgc gcgcctggtc
      121 cagaaggtcc ttgccgaagg cgtacttctt cacgaagttg tcgtacaccg gctggatacg
      181 cttttcgaag gtgtgcttgt ccacgtcggt gacgatctgg acccccttct ccttcaggaa
      241 cccgatggcc ttgttctcga gtgcgatgta ggtgtcaagg aagtacttct ccgtctccgg
      301 tccggccgcg atcatctcgg cctggatgtc cttgggcagg ccgtcccacc acttcttgtt
      361 ggcgatgaag ccgctcaccg accagaactg ctcggtgaga gaatagacct tggccacctc
      421 ccagtgcttg gaggtccaca gcagggcggg ggagttctcg gcgccgtcca ggaccttctg
      481 ctggagggcc aggtatacct cgccgaaggc cagcggggtg ccgatgcctc ccatctcgtt
      541 gatggcctgg atctgaatgt cgctgggctg aacgcggatc ttgagcccct tcaggtcttc
      601 cggcttccgg atgggcttgt gcgtgtatat actgcggccg ccgtcatcta cccagaagag
      661 ccccgccagg ccctgcttgg gatagaaatc ggtgaagaat ttctttccca cgttgcttcg
      721 gatgaccttc cacaactcct cacgactgtt gaagaggtag gggagatccc acaccttgga
      781 ctcgggaatc cactcacccg agaaggacat gggcatcttg gccatctcga tgctgccggt
      841 ctggacgccc atgaacaggt ccttggtctg gccaagctgg gcggcgggga aaacctggat
      901 ctccatccgc cccttggtcc gctcctttac cctgtcggcg aagaatttca tggtcaggga
      961 gtgcgggtga tccggcgaca ggatgtccgc gatctttgcg acgatcttct tctcctgggc
     1021 ctgggcgctc cccagggcgc acgcgaaggc caccagcacc gccagggcca cgatcaagaa
     1081 ctgcctggat ctggacattg ccaaacctcc tgaaaccccc ctcgggttga tgaaggccgc
     1141 tcgcacggca ggcgcccgcc tcttctgggg ccccggtgca ggctagattt tttacccttt
     1201 caaataccta tattctctct gcggtgtcaa ggcaaagcac cgggaagtac ggtctccggg
     1261 acatggggag cacaatcgta gtccggcatg tcatgggtta cccaatcccg aagagcgtga
     1321 tgaggccgta actcacaccc gccatcagga cagccgcgca caggccggca taaaacggcc
     1381 tcagcccgat gcgcctcatg gaggcgatgt tggtctccag cccgaccccg gcgatggcga
     1441 cgaggatca
//
LOCUS       DBNGZC010000491         1890 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_724828, whole genome shotgun sequence.
ACCESSION   DBNGZC010000491 DBNGZC010000000
VERSION     DBNGZC010000491.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_724828"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..398)
                     /locus_tag="AC16G0_08650"
     CDS             complement(60..398)
                     /locus_tag="AC16G0_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099716.1"
                     /translation="MEQFFVPVLVGLTSLGGFLLGARALGLSGRGLRAAVGKTLECVG
                     LTLVFFLTNLVAGMAVVLLGRLLTRGFASLYLASDVTLLVLSLLQGLTFQWWRERQIS
                     SGERMLGSEG"
     gene            complement(414..1097)
                     /locus_tag="AC16G0_08655"
     CDS             complement(414..1097)
                     /locus_tag="AC16G0_08655"
                     /inference="COORDINATES: protein motif:HMM:NF014724.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR5099717.1"
                     /translation="MQHEPGEDVKPTGAPVAGPSGPGEWECVCRALGLVGVACFIAIA
                     FTPLPNFISTRLEIPSQLESAGAIVVLGAGVSKDGVLGEDSLRRAIQGVLLQRKGLAP
                     LLVLSGPAFKNGPTEAAVRAELARELGVPSNAILTEETARTTREEAIRIGALLRARQV
                     RRILLVTSPYHMLRAQRLFERAGFEVLPATAEDGSRTDKSPGGRLKFMRQVLQEVLAR
                     LYYQAAGYL"
     gene            complement(1304..>1890)
                     /locus_tag="AC16G0_08660"
     CDS             complement(1304..>1890)
                     /locus_tag="AC16G0_08660"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR5099718.1"
                     /translation="ADMGAAMELRRSLNAIDPDAEKLSVNDIIIKATTLALQRHPAIN
                     AAFHDGQVRLFKRVHMGVAVALEDGLIVPVIRDCERKSLGQIAREARSLFERARNKKL
                     RPEEYSGGTFAISNLGMFDVVEFTAVIDPAHGAILAVGAVEEKPVAMDGQIVVRQRLR
                     LTGAFDHRIIDGAMGAKFLQELKKILENPVQLLL"
BASE COUNT          367 a          661 c          551 g          311 t
ORIGIN      
        1 ttagcatcgt tcatgcccaa ctcctctcgc cagagcaaac atcaaaaccg atctgcctcc
       61 taaccctcgc tccccagcat ccgctcccca gatgaaatct gtcgttcccg ccaccactgg
      121 aaggtgaggc cctggagcag cgaaagcacc agcagcgtca cgtcgctggc caggtagagg
      181 gaagcgaatc ctcgcgtcag cagccgcccg agcaacacga cggccatccc cgctacgagg
      241 ttcgtcaaga agaagacgag cgtcaatccc acgcattcca gtgtcttccc gactgccgcg
      301 cgcaggcccc tccccgaaag cccgagcgct cttgctccga gcaggaaacc ccccagggac
      361 gtgaggccga ccaagaccgg tacgaagaac tgctccatac tctcctccca ccctcacagg
      421 taacccgccg cctgatagta gagacgtgcg agaacctcct gaaggacttg gcgcatgaac
      481 ttgagacgac ccccgggcga tttgtcggtg cgcgagccgt cctccgccgt ggccggaagc
      541 acctcgaagc cggcacgctc aaacagacgt tgagcacgca gcatgtggta cgggctggtg
      601 acgagaagga tccgccgcac ctgtcgggcg cgcaggagcg ctccgattcg aatcgcctcc
      661 tcgcgcgttg tcctagctgt ctcctctgtc aagatcgcat ttgacggcac gccaagctct
      721 cgagccagtt cggctcggac cgccgcctct gtcggcccat tcttgaaggc cggcccggac
      781 agaacgagga gcggtgcaag gcctttgcgc tgcagaagaa caccctgaat tgcccggcgg
      841 agcgagtcct ccccgagcac gccatccttg gaaacgccgg cccccaagac gacgatcgcc
      901 ccggccgact caagctgcga gggaatctcc agcctggtgc tgatgaagtt cgggagtggg
      961 gtgaaggcaa tggcgatgaa gcaggcgact ccgacgagtc ccagggcccg gcagacgcat
     1021 tcccattccc ctggaccaga aggcccggca accggggcgc cggtaggctt cacatcttcg
     1081 ccgggctcat gctgcaccac cgttccccct cacacaccat cacagcattc aaggcgtcct
     1141 gggctcgtca aatcggcctc gcctgtcgcc agggagaaat gcggcggcag gcgacgaaaa
     1201 aaggccgcgc gcctggcagg aaggctgacc ttccgtccac agcgggcggc ctcgtgagac
     1261 aggcgacctc ctcggcgact gtcgagggtg tctccccctc gaatcacagc agcaactgca
     1321 ccgggttctc caaaatcttc ttgagttcct ggaggaactt tgcgcccatg gcgccgtcga
     1381 tgatccggtg gtcgaaggcg ccggtgaggc ggagccgctg ccgcaccacg atctgtccat
     1441 ccatcgccac gggcttctct tcgacggcgc cgaccgccag gatggcccca tgggcgggat
     1501 cgatcacggc agtgaactcc accacgtcga acatccccaa attggagatg gcaaaggtcc
     1561 ccccggagta ctcctccggt cgcagcttct tattccgcgc ccgctcgaaa aggctgcggg
     1621 cctcgcgggc gatctgaccc aggctcttcc gctcgcagtc gcgaatcacg gggacgatca
     1681 gcccatcctc cagggccacc gccaccccca tgtgcacccg cttgaacagt cgcacctgtc
     1741 cgtcgtggaa ggcggcgttg atcgcgggat ggcgctgcag cgccagcgtc gttgccttga
     1801 tgatgatgtc gttgacactg agcttctcgg catcgggatc gatggcgttc agggatctgc
     1861 ggagctccat agccgccccc atgtccgcct
//
LOCUS       DBNGZC010000492         2381 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1215552, whole genome shotgun sequence.
ACCESSION   DBNGZC010000492 DBNGZC010000000
VERSION     DBNGZC010000492.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1215552"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..585)
                     /locus_tag="AC16G0_08665"
     CDS             complement(<1..585)
                     /locus_tag="AC16G0_08665"
                     /inference="COORDINATES: protein motif:HMM:NF013106.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HIR5099719.1"
                     /translation="MIRWAGMVMIVAAGTCLWAAFGTPPPSVPPFESVRAAHHPSDVE
                     LLDRNGELLHELRVGETARRLAWASLSEISPALQAAVLASEDRRFYRHGGVDVKALAA
                     ALLQRIRGGPPRGASTISMQVAALTSPSLRRGGGPRTLAQKWRQMRAAWAMEQRWSKA
                     EILEAYLNRVTFRGEVQGITAAAGVLFGKAPHGIT"
     gene            complement(582..>2381)
                     /locus_tag="AC16G0_08670"
     CDS             complement(582..>2381)
                     /locus_tag="AC16G0_08670"
                     /inference="COORDINATES: protein motif:HMM:NF036531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HIR5099720.1"
                     /translation="EGDRFQSEFTVRNATDRSMDILVRGRVEGLPGTLEPRTIRLAAG
                     EAKTLGWEIAVPTGIRTLTHEVQADERAGASDRVRVTQQVRQAVPVRTYQATLSRWEQ
                     VMQQPVERPADAIPGRGGVQVVLGSTLVEELAGVREYMTRYPYTCLEQEVSRVVALRD
                     EDRWKQLAARLPAFLDSDGLLKYFPQMWTGSEVLTAYVMAVIHESGWTFPTDVRQRME
                     RGLQRFVEGAIFRRSALPTADLSIRKLAALEALSRYGKAEPRLLGSVTIEPNLWPTSA
                     VLDWWNLLHRVAGIPLREARLREAEQIIRARLNLQGTTLGFSTEQTDDLWWLMVSPDV
                     NALRLILHLLEASEWRDDLPRIVRGGLGRQHRGAWGLTVANAWGALAVEKFTRTFEKA
                     PVSGTTTAGLAGAKESLDWAQTPKGKTLAFPWPPSGQDLVVSHAGSGNPWVTVQGLAA
                     IPLRTPLSSGYRITRTVTPIQQRQPGRWSRGDILRIQLSVLAQSDMTWVVVNDPIPAG
                     ASHLGTGLQRDSSIAVQGEKLEGWAWPAFEERSFEAFRAYYEFVPKGSFTIEYTLRLN
                     QSGRFQLPTTRVEALYAPEMFGELPNAPLEVLP"
BASE COUNT          373 a          841 c          790 g          377 t
ORIGIN      
        1 cgtgatccca tggggggcct ttccgaagag cacgcccgcc gccgctgtga ttccctggac
       61 ctctccccgg aaggtcaccc ggttgagata ggcctccagg atctctgcct tggaccagcg
      121 ctgttccatg gcccaggcgg cccgcatctg ccgccatttt tgcgccaagg tcctggggcc
      181 gccgccgcgg cgcaggctcg ggcttgtcaa cgcggcgacc tgcatgctga tggtgctggc
      241 gcctcgaggt ggtccgcctc tgatccgctg gagcagggcg gcagccaggg ccttcacatc
      301 cacgcccccg tgacggtaga accgccgatc ctcggaggcc agcacggcgg cctgcaaggc
      361 gggggaaatc tccgagaggc tcgcccaggc cagccgcctg gccgtctcgc cgacccggag
      421 ttcatgcagc agctcaccgt ttcgatccaa tagctccacg tcggaagggt ggtgggccgc
      481 acgcacagac tcgaaggggg gcacgctggg cgggggtgtc ccgaaggccg cccagagaca
      541 tgtgccggcg gcgacgatca tcaccatgcc tgcccagcgg atcacggcaa gacctccagc
      601 ggggcgttcg gcaattcccc gaacatctcc ggcgcgtaca gggcctcgac gcgcgtcgtg
      661 ggaagctgga agcgtccgct ctggttgagc cggagcgtgt actcgatggt gaagctaccc
      721 ttggggacga actcgtagta ggcacgaaag gcctcgaagg acctctcttc gaatgccggc
      781 caggcccagc cctccagctt ctctccctgc acggcgatgg aagagtcccg ctgaagaccc
      841 gtgccgaggt gtgaggcgcc cgccgggatc ggatcattca ccacgaccca cgtcatgtcg
      901 ctctgggcca ggacgctgag ctggatccgc aggatgtccc cccggctcca gcggcccggt
      961 tgcctctgct ggatcggcgt cacggtccgg gtgatccggt agccgctgga aagcggcgtc
     1021 ctgagcggga tggccgccag tccctggacg gtcacccacg ggttcccgct gcctgcgtga
     1081 ctcaccacga gatcctgacc cgacggtggc caggggaagg ccagggtctt tcccttcggg
     1141 gtctgcgccc agtccagcga ctctttggcc ccggccagac cggccgtggt cgtgccggag
     1201 accggggcct tctcgaatgt ccgggtaaac ttctcgacgg ccaaggcccc ccaggcattg
     1261 gccacggtga gaccccaggc gccgcggtgc tggcggccca gcccaccccg gacgatccgc
     1321 ggcaggtcgt cccgccactc gctcgcctcc agcaggtgca ggatcaaacg caacgcgttc
     1381 acatccgggg acaccatcag ccaccagagg tcatccgtct gctccgtgga gaagcccagg
     1441 gtggttccct gcaggttcaa gcgggcgcgg atgatctgct cggcctcgcg cagccgcgcc
     1501 tctcgaagcg ggatgccggc tacgcggtgc aagaggttcc accaatccag cacggcggac
     1561 gtcggccaga ggtttggctc gatcgtgacg ctgccgagga gccggggctc cgcctttccg
     1621 tagcgggaga gggcctccag ggcggcgagc ttccggatgg agaggtcggc cgtgggcagg
     1681 gccgagcggc ggaagatggc cccctcgacg aaccgctgca atcccctctc catccgctgt
     1741 cgcacgtcgg tcgggaacgt ccatcccgat tcgtggatca cggccatgac gtaggcggtc
     1801 aggacctcgc tccccgtcca catctgcggg aagtacttga ggaggccatc cgagtccagg
     1861 aaggcgggaa gacgggcagc cagttgcttc cagcgatcct catcgcgaag cgcgaccacc
     1921 cgggagacct cctgctccag gcaggtgtaa gggtagcgag tcatgtactc ccgcacgccg
     1981 gccagctcct ccaccagcgt cgagccgagc acgacctgca ctcccccccg ccccggtatg
     2041 gcatcggccg ggcgctcgac cggctgttgc atgacctgct cccagcggga gagcgtggcc
     2101 tggtacgtcc ggacagggac ggcctggcgc acctgctggg tcacccgcac ccggtcactc
     2161 gcgccggccc gctcgtcggc ctgcacctcg tgagtcagcg tccggatgcc cgtggggaca
     2221 gcgatctccc accccaacgt cttggcttcc ccggcggcga ggcggatcgt cctcggctcc
     2281 agggttccag gcagtccttc cacccggccg cggacgagaa tgtccatgct ccgatccgtg
     2341 gcgttccgga cggtgaattc cgactggaat cgatcgccct c
//
LOCUS       DBNGZC010000493         2340 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1997213, whole genome shotgun sequence.
ACCESSION   DBNGZC010000493 DBNGZC010000000
VERSION     DBNGZC010000493.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1997213"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="AC16G0_08675"
     CDS             complement(<1..807)
                     /locus_tag="AC16G0_08675"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR5099721.1"
                     /translation="MKKPRVSRSIVLAVLLMFGLAAVQAVAADKPKDYPNKPVTIQVG
                     FGAGGSSDVGVRVLAEALKKIIGQPVLVENKPGAGSQVMLSDFKNNAKPDGYTLALIN
                     IPQLQTIVFDPTRKASFGMKDFQPVANHVQDPGAILVRNESPFKTLEDFLQEAKAKPG
                     QVKASTTGIGSDDHLAVLDLQRKANIRFNIIHLQDTPAALTAALGGHTDVDFDNVGGF
                     LPSAKSGTARILAVMMEQRYPDLPDVPTFKERGIDLVSSSTRGYVFPAGTP"
     gene            complement(1010..>2340)
                     /locus_tag="AC16G0_08680"
     CDS             complement(1010..>2340)
                     /locus_tag="AC16G0_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012861.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HIR5099722.1"
                     /translation="EGPAAQISSGAASLLCDILKVSPAERRMLVLAGMAAGLSAIFRA
                     PLGMAIFAGEILYAGLAFEFEALQYTLISAVVAYAVNGLIVGWAPLFLLPSNLTFTRP
                     VALLGYAILGVVAGIVGALEPPIFYGIRDFFSQLRVPRHVKPAIGGLLMGLVAMVFPQ
                     TLSTGFGWVQMALSADYIGPQLLLLPFAKILAMSFAIASGGSGGVFGPNVYIGAMLGG
                     AVAYVMDLWFPAAHFVPAAFVVVGMGAVFVGTARVPLSTLIMVAEMTGGYGLIVPSML
                     ASVLSFTVQRTVGAQFRYSHLYESQVEGRLDSPVHHQRLVQGALQILEAKTIRDLPGI
                     SLPDLSNLLRFGQPINIHGGKGRVFAFALDSEDGSLVGRDAEEAFGSLRDVTLIAIVR
                     SEEVLPSGQVGPLEQGDQLIIAASEEAYGEFLRAQAEPGPTKPAGPPDGT"
BASE COUNT          433 a          739 c          776 g          392 t
ORIGIN      
        1 gggggtcccg gccgggaaca catagccccg ggtggaagag gagacgaggt cgattccccg
       61 ctccttgaac gtgggcacgt ccggcaggtc cgggtaccgc tgctccatca tcaccgccag
      121 gatccgtgct gtcccggact tggccgaagg caggaagcct cccacgttgt cgaagtccac
      181 atcggtgtgt ccgcccaggg ccgccgtgag cgccgcgggg gtgtcctgca ggtgaatgat
      241 gttgaagcgg atgttcgcct tgcgctgcag gtccaggacc gccagatgat cgtcgctgcc
      301 gattcccgtc gtcgaggcct tgacctggcc cggcttggcc ttggcttcct gcaggaagtc
      361 ctccagcgtc ttgaaggggc tttcattccg gaccaggatg gcccccgggt cctgcacgtg
      421 gtttgccacg ggctggaagt ccttcatgcc gaaggaggct ttccgggtcg ggtcaaacac
      481 gatggtctga agctggggaa tgttgatcaa tgccagcgtg tacccgtcgg gcttggcgtt
      541 gttcttgaag tcgctgagca tcacctggct gccggctccc ggcttgttct ccaccaggac
      601 gggctgcccg attatcttct tgagggcctc ggccaggacg cggaccccca cgtcgctgct
      661 cccgcccgct ccaaagccga cctggatggt gacgggcttg ttcggatagt ccttcggctt
      721 gtcggcggca acagcctgaa cggctgccaa gccgaacatg aggaggactg caaggacgat
      781 cgaccgggac acgcgtggct tcttcatcat tttctccttc cgcaagaatt cccgtccggg
      841 ctcgatccaa ctcacggggg aaaaatcggg cgtctgaaga ctcaggtgtg tctatactat
      901 cgagggtgag tgcacggcaa tcccgttcac tggggacgga cgcctttgtt cggtccagtg
      961 aacctaccgg tccgcccgag gcctttgccg tcgaaaggat ggatgggtct caggtgccat
     1021 cgggcggacc ggctggtttc gtcggaccgg gctcggcctg ggcccggagg aattcgccgt
     1081 aggcttcctc gctcgcggcg atgatcagct ggtcgccctg ctcgagcggc ccgacttggc
     1141 ccgagggcag aacctcctcg ctccgcacga tcgcaatgag ggtcacatcc cggagcgatc
     1201 cgaaggcctc ttcggcgtca cggcccacca gggagccgtc ctcggaatcc agggcgaagg
     1261 caaagacgcg tcccttgccg ccgtggatgt tgatgggctg cccgaaacgc agcagattgc
     1321 tcagatcggg taggctgatt cccggaaggt cccggatcgt cttcgcctcc aggatctgca
     1381 aggcaccctg gaccagacgc tggtgatgca cgggggagtc cagtctccct tccacctgcg
     1441 actcgtacag gtgcgaatag cgaaactgcg ccccgacggt ccgctggacc gtgaagctca
     1501 ggaccgaggc cagcatggat ggcacgatga gtccgtagcc tccggtcatc tcggccacca
     1561 tgatcagcgt ggacaggggc acccgggctg tcccgacaaa gaccgcaccc atgccgacga
     1621 cgacgaaggc cgcgggcacg aagtgtgcgg cgggaaacca caggtccatg acgtaggcca
     1681 cagcgccccc cagcatggcc ccgatgtaca cgttggggcc gaagacgcct cccgagcccc
     1741 cggaggcgat ggcgaatgac atggccagga tctttgcgaa gggcaagagc aggagctggg
     1801 ggccgatgta gtcggcggac agcgccatct gcacccatcc gaatcctgtg gacagggtct
     1861 gcgggaagac catggccacg agacccatca gcaggccgcc gatggccggc ttgacgtgtc
     1921 tggggacccg aagctggctg aagaagtccc ggatgccgta aaagatcggg ggttccaggg
     1981 ctcccacgat cccagccacg acgccaagga tggcgtaccc caggagggcc acgggacggg
     2041 tgaaggtaag gttggagggc aggaggaaca ggggcgccca gccgacgatg agtccgttca
     2101 cggcgtaagc cacgacggcg gagatgagcg tgtactgcag cgcctcgaac tcgaaggcta
     2161 ggcctgcgta gagaatctcc ccggcaaaga tggccatccc cagaggcgcg cggaagatgg
     2221 ccgaaagccc cgccgccatc cccgccagca ccagcatccg ccgctcggca ggagagacct
     2281 taaggatgtc acacagcaag gaggccgcac cggagctgat ctgtgccgcc ggaccctccc
//
LOCUS       DBNGZC010000494         2644 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1703972, whole genome shotgun sequence.
ACCESSION   DBNGZC010000494 DBNGZC010000000
VERSION     DBNGZC010000494.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1703972"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1242)
                     /locus_tag="AC16G0_08685"
     CDS             complement(163..1242)
                     /locus_tag="AC16G0_08685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HIR5099723.1"
                     /translation="MPYRIPIGPFHPALEEPYKLSVSCQGETIQGVQIEIGFSFRAIE
                     LLAQRRNYIQGLTLVERVCGICSGVHTLTFCMAAEVLAGVRVPPRGHYIRVIVAELER
                     LHSHLLWAGIAADDMGFQSLFMEVFALRERIMDVLELISGNRVHYAMNCIGGVNRDVR
                     DPEAVLAAVRGVRQVLEQDVVSVFSLDPTVRARTQGVGILGREQAIAYGVVGPTARAS
                     GLDMDVRRDQPYAAYGELGVQVVTADGGDIQARIMVRALEMQESIRLIQEALCRLPSG
                     PTRADTGIPVLPPGEATARAEAPRGELFYYLDSDGSDTPRRVKIRTPTFVNIPAVEAM
                     CLGQHFADLPIIQASVDPCCSCTDR"
     gene            complement(1288..1731)
                     /locus_tag="AC16G0_08690"
     CDS             complement(1288..1731)
                     /locus_tag="AC16G0_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012549.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR5099724.1"
                     /translation="MTEAGELREAGTWLRDVPGAAPWERRQDGWWMRAPLLDVMAMAE
                     AMNQLGARLSTMTASALEGGETAVIYHYVLGSEAINIRTQTRDRSLPSIAPVTLASGW
                     IEREIQDLYGTEFPGHPNLTRLVRPAEVPQGFFRDPEDAKKGRQA"
     gene            complement(1724..2245)
                     /locus_tag="AC16G0_08695"
     CDS             complement(1724..2245)
                     /locus_tag="AC16G0_08695"
                     /inference="COORDINATES: protein motif:HMM:NF024247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR5099725.1"
                     /translation="MGIWKILTQNAGRGSRTRSPSDMVPSPEAFRGALQHSLDRCTGC
                     QICAYVCSPTAISFKTCDQHSTVWGYFAGQCTFCGRCVEYCPTGALTFEGDAPPVTGD
                     PAHHRLAHEVVFPLCPRCGRSVQPMPEALLVRLYKEPLSREMQARQGLCERCRARTMA
                     EALKVQVGGRRHD"
     gene            complement(2270..>2644)
                     /locus_tag="AC16G0_08700"
     CDS             complement(2270..>2644)
                     /locus_tag="AC16G0_08700"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HIR5099726.1"
                     /translation="AAVALLLAIPARLKRNPFSIPNAEQEIVAGSLTDYSGAPLALFE
                     LAHSLELVALVGLSAVLLLPPAGGPALAAGLYFCGSMALVVLVTLVAAATARLKVQQA
                     FRFYWSWGALAGLAALVLAAIG"
BASE COUNT          489 a          904 c          830 g          421 t
ORIGIN      
        1 acgccgcaga tgggcctttt tcagcagcct gttgggggcg ttgccgcgtc ttgacccatt
       61 agacaaggaa ggggtggaaa aggttccagt gcaaccagac ttcttgggaa tgaaatgcgg
      121 gaaggatgac tcaaaggcaa aaccagggcg gcaggccgct ttctacctgt cggtgcagga
      181 gcagcagggg tccaccgacg cctggatgat cggcaggtcg gcgaagtgtt ggcccaagca
      241 catcgcctcg acggcaggga tgttcacgaa ggtgggcgtg cggatcttga cgcgtcttgg
      301 ggtgtcgctc ccgtcggagt ccagatagta gaagagttct ccccgcgggg cctccgcccg
      361 ggcggtggct tcgccgggag gaaggaccgg gatgcccgtg tcggcgcgcg tggggccgga
      421 gggcagcctg catagcgcct cctggatgag gcggatgctc tcctgcattt ccagcgcgcg
      481 gaccatgatt cgcgcctgga tatcgccgcc atcagccgtc acgacctgga cccccagctc
      541 cccgtaggct gcatacggct gatcgcgccg cacgtccatg tccagcccgg aggctcgggc
      601 cgtcggaccc acgacgccgt aggcgatggc ctgctcgcgg ccgagaatcc ccaccccttg
      661 ggtgcgtgcc cggacagtgg gatccaggct gaagacggaa acgacatcct gctccagcac
      721 ctgccgcacc ccgcgcaccg ccgccagcac agcctccgga tcccgcacgt cgcgattcac
      781 gccgccgatg caattcatcg cgtagtgcac ccgattccca gagatgagct ccaagacatc
      841 catgatccgt tcccgcaggg caaaaacctc catgaacagg ctctggaaac ccatgtcgtc
      901 ggccgcgatt cccgcccaca gcaggtgcga gtgaagtcgt tccagttccg ccacgatgac
      961 ccgaatgtag tggccccgcg gaggcacgcg gacgccagcc aggacttccg ccgccatgca
     1021 gaacgtcagg gtgtgcacgc cggaacagat cccacacacc cgctccacca gcgtgaggcc
     1081 ctggatgtag ttccgccgct gggccaggag ttcgatggcc cggaagctga atccgatctc
     1141 gatctgcacc ccttggatcg tctcaccctg gcaggacacg ctgagcttgt agggctcctc
     1201 cagcgctggg tgaaatggcc cgatgggtat ccggtacggc atccggttct ccgaaaactc
     1261 cagacctggc tcgcaccccg aagccgctca ggcctgcctc cccttcttgg catcctctgg
     1321 gtcacggaaa aagccctggg gcacctccgc gggacggacc agccgggtca gattcgggtg
     1381 gccgggaaac tccgtcccgt agagatcctg gatctcccgc tcgatccagc ccgacgctag
     1441 tgtcaccggc gcgatagagg gcagcgaccg atcccgcgtc tgcgtcctga tattgatggc
     1501 ctcagatccc agaacatagt gatagatcac cgccgtctcg cccccttcca gcgctgaggc
     1561 ggtcatcgtg ctgagacggg ccccgagttg attcatggcc tcggccatcg ccatgacatc
     1621 caggagggga gcccgcatcc accacccgtc ctgccgacgc tcccatgggg cggcccccgg
     1681 gacgtcacgg agccacgtgc ccgcttcccg cagctcgcct gcttcagtca tggcgtcggc
     1741 ctccgacctg gaccttcagg gcttccgcca tcgtcctggc ccgacaccgc tcacacaatc
     1801 cctgacgggc ctgcatctct cgagacagcg gctccttgta cagccgcacc agaagcgcct
     1861 ccggcatcgg ttgaacggat cggccacacc gcgggcaaag aggaaatacg acctcatgcg
     1921 cgaggcggtg gtgcgcggga tctcccgtca ccggcggggc atccccttca aaggtgagtg
     1981 ctccggtcgg gcagtattcc acacagcgtc cgcaaaaggt gcactggccg gcgaagtagc
     2041 cccacaccgt cgaatgctga tcgcacgtct tgaagctgat ggccgttggc gagcacacat
     2101 aggcgcagat ctggcagccc gtgcaccggt ccaggctgtg ctgcagcgcc ccacgaaatg
     2161 cctcggggga cggcaccatg tcggagggcg atcgcgtgcg cgaaccccga ccggcgttct
     2221 gcgtgagaat tttccagata cccaccgttt ctcctcgaac ctccagacct tacccgatcg
     2281 cagccaatac caatgccgcc aggcccgcca gggcgcccca gctccagtag aagcggaagg
     2341 cctgctggac cttgaggcgg gccgtggctg cagccactag ggtcaccaaa actaccaggg
     2401 ccatgctccc gcagaagtag agtcctgccg ccagggcagg gccgccggca ggcggcagga
     2461 gcaggaccgc ggagagcccg acgagcgcca ccagctccag gctgtgagcc agctcgaaga
     2521 gggccagggg ggcgccggaa tagtcggtga gcgatccggc cacgatctct tgctccgcat
     2581 tggggatcga gaacggattc cgcttcagcc gggctggaat cgccagcagc agggccacgg
     2641 ccgc
//
LOCUS       DBNGZC010000495         2880 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_529907, whole genome shotgun sequence.
ACCESSION   DBNGZC010000495 DBNGZC010000000
VERSION     DBNGZC010000495.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_529907"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1541
                     /locus_tag="AC16G0_08705"
     CDS             120..1541
                     /locus_tag="AC16G0_08705"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF012722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5099727.1"
                     /translation="MPTDTDRFQTTVEWSFAVLRALAMLGGMIALFLVPLHPEHQPHV
                     APLAWTFVAYKALLFLAIWMWPGRLRLILLGTTGLDLVFVSLFVWHTGGAESHFYLLF
                     YILVALAAAHFGPGIGLTTAGGGGLLYLLASVVGAPVTDWNHLIARAATFFLLGGSLG
                     YLSQRERLARAETERLNEELREHQARLEKAYQELQAAQAMLVQSERLAAIGQMSAKVS
                     HEVRNPLSSISLNAELLEDELQALPPEPRAEAANLMAAIRSQVDVLSAVTEEYLRFAR
                     LPKPKLEAKALAPVIEDLAEFVREELRARKVSLEVDVADGLPVLRLDAGQIRQALLNL
                     IRNAAEEMSGGGRITVSAAVGHRAQSTGHRAQGTGHRAQGSGREVGEAGGDSLSMPSG
                     DSVLPAVFIEVRDTGTGISAENLRKIFEPFFTSKGGGTGLGLPIARQIALDHGGTLTC
                     ENAPEGGAIFLLTLPIPNGGRTP"
     gene            1538..>2880
                     /locus_tag="AC16G0_08710"
     CDS             1538..>2880
                     /locus_tag="AC16G0_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020742225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR5099728.1"
                     /translation="MTADAARIRVLVVDDDQAIREALTRTLEKLGYGVAVAADGQEGL
                     DRLRQGGVHILLADLKMPRISGQELLKAAKAIAPDIEVIVITGHGTVEDAVEAMKEGA
                     YDFITKPFKRVQLEKTITKAAEKQGLVLQNRDLQQRLDALQGAGQIVGGSPAMLRTLD
                     LVTQVAPSAATVLIQGESGTGKELIANAVHHGSPRRDRPFIKVNCAALPETLLESELF
                     GDEKGAFTGAAARKEGRFELADGGTLFMDEIGDLSPATQAKLLRVLQEGEFERVGGTR
                     TLKTDVRLVAATNADLATLVREKRFREDLFYRLNVITIQIPPLRARREDIPLLAQHFL
                     RRFAVKNAKTITGFTDEAMDLLQTYTWPGNVRELENVIERAVVLTRASMISHADFPET
                     VVGSDQAARHLVISIGTPLEEVEDRLIDETLRYTKGDKTLAAKLLGIATRTIYRR"
BASE COUNT          530 a          908 c          974 g          468 t
ORIGIN      
        1 gggaagaaca tgtgctatac tccccacaat ctcgaatcag cccttatcgc gttcggaagc
       61 ttggtgcgtc gccgacactc ggaacacgac agctgccttc agcggagttg ctggcacaga
      121 tgccgaccga cacagataga tttcagacga ccgtggagtg gagtttcgcg gtcctgcgcg
      181 ctcttgccat gctggggggc atgattgccc tcttcctggt accgctccat cctgaacacc
      241 aaccgcacgt cgcaccgctg gcttggacct tcgtggcgta caaggcgctc ctgttcctcg
      301 ccatctggat gtggcccggc cgcttgcgcc ttatcctctt ggggacgacc ggtctcgacc
      361 tggtgttcgt ctcgctcttc gtctggcaca ccggaggcgc agagagtcac ttctatctcc
      421 tgttttatat cttggtggcg ctggccgccg cccacttcgg ccccgggatc ggcctgacca
      481 cggccggcgg tgggggcctg ctgtatctgt tggcctcggt ggtgggtgcg ccggtaaccg
      541 actggaatca cctgatcgcg cgcgcggcca ccttcttctt gctgggcgga tcgctgggat
      601 atttgagcca gcgagagcgc ctggcgcgcg ccgagaccga gcggctgaac gaggaactcc
      661 gcgagcacca ggcgcgactg gaaaaagcgt accaggaatt gcaggcggca caggccatgt
      721 tggtgcagtc tgagcgcctg gctgcgatcg gccagatgtc cgcgaaggtg tcccacgagg
      781 tgcgaaatcc cttgagctcc atcagtctga atgcggagct actggaagat gagctgcagg
      841 cgctgccgcc agagccgcgg gccgaggcgg cgaacctcat ggcggcgatc cggtcccagg
      901 ttgacgtgct gagcgcggtg accgaggaat atctgcgctt cgcgagactg cccaagccca
      961 agctcgaggc caaggcgctc gcccccgtga tcgaggacct ggcggagttc gtgcgcgagg
     1021 aattgcgggc caggaaggta agtctcgagg tggacgtggc cgacggcttg cctgtcctgc
     1081 gcctggatgc gggccagatc cggcaagcct tgctcaacct gatccggaac gcggccgagg
     1141 agatgtccgg cggtgggagg atcacggtga gtgcggcagt cgggcacagg gcacagagca
     1201 cagggcacag agcacagggc acagggcaca gagcacaggg cagcggccgg gaagtggggg
     1261 aggctggcgg ggattcgttg tcgatgcctt ctggcgacag cgttctgcct gctgtcttca
     1321 tcgaggtccg cgatacgggg acgggtatct cggcggagaa cctgaggaag atcttcgaac
     1381 cgttcttcac cagcaaagga ggcggtacgg gcttgggcct acccatcgct cgccagatcg
     1441 ccctggatca cggcggaacc ctgacctgcg agaacgcgcc ggaaggcggc gccatcttcc
     1501 tcctgaccct cccgatcccc aatggagggc gcacaccatg acggcagatg cggcccgtat
     1561 ccgggtcctg gtcgtggacg acgaccaggc catccgtgaa gccctgacgc ggacgctgga
     1621 gaagctcgga tacggcgtgg cagtcgccgc ggacggccag gagggactgg accgcctgcg
     1681 gcaagggggc gtccacatcc tgctggccga tttgaaaatg ccgaggatct cggggcagga
     1741 actgctgaag gccgccaagg ccatcgcgcc tgacatcgag gtgatcgtca tcacgggcca
     1801 cgggacggtc gaggatgcgg tcgaggccat gaaggagggg gcctacgact tcatcaccaa
     1861 gccgttcaag cgcgtgcaac tggagaagac gatcaccaag gcggcggaga agcagggact
     1921 ggtgctccag aaccgcgacc tccaacagcg gctcgacgcg ctacagggcg cggggcagat
     1981 cgtcggcggc agcccggcca tgcttcgcac cctggacctc gtaacccagg tggcgcccag
     2041 cgccgccacg gtcctcatcc agggggagag cggcacgggg aaagaactga ttgccaacgc
     2101 cgtccatcac ggaagcccgc ggcgggaccg gcccttcatc aaggtgaact gcgccgccct
     2161 gcccgagacg ctcctggagt cggagctgtt cggcgatgag aagggagcct tcaccggcgc
     2221 cgcggcccgc aaggagggac gcttcgagct ggcggacggc ggcacgctgt tcatggacga
     2281 gatcggcgac ctcagcccgg cgacacaggc caagctcttg cgggtcctcc aggaggggga
     2341 gttcgagcgc gtgggcggca cgcggacgct gaagacggat gtccgactcg tggcggccac
     2401 caatgccgac cttgccacgc tggtccggga aaagcggttt cgcgaggacc tcttttatcg
     2461 gctgaacgtc atcaccatcc agattccgcc gctccgggcg cgccgggagg atatcccgct
     2521 cctcgcccag cacttcctcc ggcgcttcgc cgtcaagaac gccaagacca ttaccggctt
     2581 cacggacgag gccatggacc tcctgcaaac atacacctgg cccggcaatg tgcgagaact
     2641 ggagaatgtc atcgagcggg ccgtggtgct gacgcgggca tccatgatct cccacgcgga
     2701 tttccccgag accgtggtgg ggagcgacca ggccgctcgc cacctggtga tctccatcgg
     2761 aacgcccctg gaggaagtgg aggatcgcct catcgatgag acgctacgct acaccaaggg
     2821 cgacaagacg ctcgccgcca agcttttggg catcgccacg cgcacgatct accggcggat
//
LOCUS       DBNGZC010000496         2825 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2389939, whole genome shotgun sequence.
ACCESSION   DBNGZC010000496 DBNGZC010000000
VERSION     DBNGZC010000496.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2389939"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..414
                     /gene="hypA"
                     /locus_tag="AC16G0_08715"
     CDS             73..414
                     /gene="hypA"
                     /locus_tag="AC16G0_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015175288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HIR5099729.1"
                     /translation="MHEVSLMQDALILAAKRASEAGGERIHRLTMRVGPLSGAVPEAL
                     EFAFEMLARGTMAEGATLEVERAPIVCYCSTCRAEFQSSDFVCECPTCHQPSADVRGG
                     RELELASLEVS"
     gene            417..1268
                     /gene="hypB"
                     /locus_tag="AC16G0_08720"
     CDS             417..1268
                     /gene="hypB"
                     /locus_tag="AC16G0_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012167693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HIR5099730.1"
                     /translation="MCKDCGCSIVGDVAIHGAEAPEHVHPHEHTHDHDHSHPHVHPHE
                     QDASSDGSRRSVPVHQAILDKNDRLAERNRGYFLAKGLLVVNVLSSPGSGKTTFLERT
                     AADLAGRLRLGVIVGDLETDNDARRLRKSGLPSVQITTGSVCHLDAEMVARAMTRLPL
                     DDLDILVIENVGNLVCPASYDLGESLRVVLLSVTEGEDKPLKYPVMFKTAHVIVVNKM
                     DIAEAVGYDREASLANIRRIAPQATIFEVSARTGQGMAQWYTHLQAGCQPEGGNPAPP
                     PGDTSLA"
     gene            complement(1384..1767)
                     /locus_tag="AC16G0_08725"
     CDS             complement(1384..1767)
                     /locus_tag="AC16G0_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099731.1"
                     /translation="METQEAREISLEMLKKFGTRWAVLAAMRLDMASRGISIGSETDE
                     QLKLARVQILSGCFSPCEVGCSLSKVEGQFISSGAALGGEYLRPWFDLLAQAMRGEID
                     PTRIAEIPALKPVAMDCEFLACHCG"
     gene            2192..>2825
                     /locus_tag="AC16G0_08730"
     CDS             2192..>2825
                     /locus_tag="AC16G0_08730"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5099732.1"
                     /translation="MSEHNTQTQNSGNGGGEEEPRIGVFICHCGGNISDVVDVRRVAE
                     EIGRLPHVVFSTTHMFICSDPGQALIEQKIKELNLNRVIVAACSPSLHELTFRRTLAR
                     AGLNQYLYEHTNIREQVSWVVEDKELATQKAIRLVSASVARVPHLAPLQKRVIPIHPS
                     ALVIGGGIAGLTAARDLAARGIEVTIIESSSFLGGRMPRLAGLFPTGRQAR"
BASE COUNT          524 a          929 c          887 g          485 t
ORIGIN      
        1 cccgaggcct tggcgcaaga ggacgggatg gtggtgtatc agcgcatgga gcacgtcgtc
       61 taggattgtg tcatgcacga ggtcagcctg atgcaggacg cactgatcct ggccgccaag
      121 cgggcgagcg aggccggggg ggagcgcatc cacagattga cgatgcgggt cggaccgctg
      181 tccggcgcgg ttcccgaggc cttggagttc gccttcgaga tgctcgcccg cgggaccatg
      241 gcggagggcg caaccctgga ggtcgagcgc gcgcccatcg tgtgttattg ctcgacctgc
      301 cgtgcggagt ttcagtcgag cgatttcgtg tgcgaatgcc cgacgtgtca ccagcccagc
      361 gccgacgtgc gtggcgggcg ggaactggaa ctggcatcgc tggaggtttc ctgaccgtgt
      421 gcaaggattg cggctgcagc atcgtcgggg atgtggccat ccacggggca gaggcccccg
      481 agcatgtcca tccccatgag catacccatg accacgacca ttcgcatccc cacgtccatc
      541 cccacgagca ggatgcgtcc tccgacggct cccgacgcag cgttcccgtg caccaggcga
      601 ttctggacaa gaatgaccgc ctcgcggagc ggaaccgggg ctacttcctg gccaagggcc
      661 ttctggtcgt gaatgttctc tcctcgcccg gttccggcaa gaccacattc ctcgagcgca
      721 cggccgcaga ccttgccggc cgcctgcgcc tcggcgtgat cgtgggcgac ctggagacgg
      781 acaacgatgc gcgacgcctc cggaagagcg gcctcccgtc ggtccagatc accaccggaa
      841 gcgtctgcca cctggacgcc gagatggtgg cccgcgccat gactcgcttg cccctcgacg
      901 acctcgacat cctggtcatc gagaacgtag gcaacctggt gtgtcccgca tcctatgatc
      961 tgggggagag tttacgggtc gtgctgctgt ccgtcaccga gggtgaggac aagccgctca
     1021 agtacccagt catgttcaag actgcgcacg tgatcgtcgt gaataagatg gatatcgccg
     1081 aggcagtcgg ttacgacagg gaggcttccc ttgcgaacat ccggcggatt gccccgcagg
     1141 cgacgatctt cgaggtgtca gcgcgaacgg gccaggggat ggcccaatgg tacacgcatc
     1201 tccaggcagg ctgccagccc gagggtggga atcccgcacc accccctgga gacaccagcc
     1261 tggcctagtt cacggcccgt tgcgacgggt atccgatcgt cagcgcgccc gggaaaatcc
     1321 ggcctgcctg gagacgggca gcattttccc ccgcgctatg tcgtgacctc ggatgcggcc
     1381 agatcagccg cagtggcaag ccagaaactc acaatccatc gccacaggct tgagggcggg
     1441 gatctccgcg attcgggtgg gatcaatctc gccgcgcatg gcctgcgcca gcagatcaaa
     1501 ccacggccga aggtactcgc ccccaagggc tgccccgctg gaaatgaact ggccctctac
     1561 cttcgagagc gagcagccga cttcgcaggg agagaaacag ccggacaaga tctgaacccg
     1621 cgccagcttg agctgctcgt ccgtctcgct cccgatgctg atgcctcggc tggccatgtc
     1681 cagcctcatg gcggccagca ccgcccaccg tgtcccgaac ttcttcagca tctccaaact
     1741 gatttcgcgt gcctcctggg tttccatgac tcccctcctc tgctcgaaat aagtcctccg
     1801 aatccctctt gtgaaaatgt acacgctctg cgggacaatg caaagcccta ttttcttgcg
     1861 ggagaggtta tgcctggacg gccgacctct cccgcggtgt tcgacacgtg agcggcggat
     1921 cccaatcaac gcatcgcagc gactgttgag aatgtaaaca gatgattggg acgatagaca
     1981 aacgcacgaa aaatccttga acgcggcgac ggggcgccta ttgtgatgcc tgagcgatgt
     2041 cgccgcagag actgaccgat gaagctcgac agttgccggg ccagccttca ggttccccgg
     2101 caccccgcac caccgtgcag ccgggcggcc gctgagtcgg aggcccgggt acggcgcacc
     2161 ggggggacac ccgttgttgg gagaggcggg catgagcgag cacaacacgc agacacagaa
     2221 ttcgggtaac ggtggcgggg aagaagaacc acgcatcggc gtcttcatct gtcactgcgg
     2281 aggaaacatc tccgacgtcg tggacgtgcg gcgtgtggca gaggagatcg gtcgattgcc
     2341 ccatgtcgtc ttctcgacca cccacatgtt catctgctcc gatcccggcc aggcgctgat
     2401 cgagcagaag atcaaggagc tgaacctcaa ccgcgtcatt gtcgccgcct gctcccccag
     2461 cctgcacgaa ctgaccttcc ggcggacgct ggcgcgcgcc ggcctgaacc aatacctgta
     2521 tgagcacacc aacatccgcg agcaggtgtc ctgggtggtt gaggacaagg agctggcgac
     2581 ccagaaggcc atccgcctgg tcagcgcctc cgtagcccgt gtcccccacc tggccccgtt
     2641 gcagaagcga gtcatcccca tccacccctc tgctctggtg atcggcgggg ggatcgccgg
     2701 cctcaccgcg gcacgggatc tggccgcgcg cggcatcgag gtcacgatca tcgaatccag
     2761 ctcgttcctc ggcggccgca tgccgcgcct ggcgggcctc tttcccaccg ggcggcaggc
     2821 acgcg
//
LOCUS       DBNGZC010000497         2259 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2586460, whole genome shotgun sequence.
ACCESSION   DBNGZC010000497 DBNGZC010000000
VERSION     DBNGZC010000497.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2586460"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..591
                     /locus_tag="AC16G0_08735"
                     /pseudo
     CDS             53..591
                     /locus_tag="AC16G0_08735"
                     /inference="COORDINATES: protein motif:HMM:NF014593.7"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
     gene            721..1323
                     /locus_tag="AC16G0_08740"
     CDS             721..1323
                     /locus_tag="AC16G0_08740"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020122009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR5099733.1"
                     /translation="MASMEELRAGVRRCLPEVEQIKNKDLRDKVVEAWALALSESEFT
                     SIDDIPPQAGPEHPLFKEGQGTQSGHFRGVARIAQGIADGLEALHPNVRIDRDLLWAC
                     GLCHDLGKPYEMSPKNQDRWKGDTATHGYPAIRHPGYGIHVALMVKLPEVVAHTAGYH
                     SMEGEWVRRSLLTTIVHHADYAYWHLVDRAGLLVTPMHLK"
     gene            1513..2238
                     /locus_tag="AC16G0_08745"
     CDS             1513..2238
                     /locus_tag="AC16G0_08745"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025464.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016837 - carbon-oxygen lyase activity,
                     acting on polysaccharides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase family 20 protein"
                     /protein_id="HIR5099734.1"
                     /translation="MKSRLLAVLLVFIWGAAASAAPVADGFESGTVGSLWRTHRVKQG
                     SATVQQGIVHSGTFALRLDLHRGDMAGLGGDGEATERAEIQEADGLWARFGESYEYSF
                     SMYLPLDFPIVPTRLVIGQWKQACQDCSKNRSPIIAQRFRDGVFFVTIETPSGRRTIY
                     QHRQSILGQWLDLRYRIRFAWSDGSVTVWLNDARVADYQGPLGYPDDPPEVYFKFGLY
                     RDRMSQPMTIYIDDYRKDRVGEK"
BASE COUNT          390 a          705 c          765 g          399 t
ORIGIN      
        1 cgaggcgatc cggaacgccg gccgcctcct cagcgagggg ggttgtcacg cggtgaagct
       61 cgaaggtggg cggcgatgtc tgcgcacgat cgagcacctg ctcgccgcga catcccggtg
      121 atggggcacc tcggtctgac gccgcagtcg ctccaccgga tgggtggctt ccgggtccag
      181 gcccgggagc ctgagggcgc ggccgcaatc ctggacgacg cgcgactgct ggcagaggcg
      241 ggagtcttcg ccatcgtcct tgaggggatt cccgcggagg ttgcccagat catcacggag
      301 agcgtctccg tccccacgat cggtatcggg gccgggccgc actgcgacgg acaggtcctc
      361 gtctggcatg atctcctcgg cctcaccgag gggctcaggc cgaagttcgt ccggcagtac
      421 gcggacctcc gaggtgtgat cgtggaagcg ctcgcccggt tcgtgggcga cgtccgcaca
      481 ggggccttcc cgtcagccga ggagagttac catgtcgctg ccgacgtggt gacggcagtt
      541 cgggaacggc agggcccttc cggtccgtcg caggagaaag aactgccgta gcggatggtc
      601 cgggcaggta cagcctgcag tgcgtccagc ggtcttcccc gtggaccacg agtgcgtagg
      661 gcatcgagga cttggatcgt cgaccgccaa gtgcaaagcg cagcaagagg aggatcatgc
      721 atggcatcca tggaggagtt gcgagcgggc gtgcggagat gtctccccga ggtggagcag
      781 atcaagaata aggatttgcg cgacaaggtc gtggaggcct gggctctcgc cttgtcagag
      841 agtgagttta cctccatcga cgacataccg ccccaggccg ggccggagca tcccctcttc
      901 aaagaggggc agggcaccca gtctggtcac ttccgcgggg tggcccgcat cgcgcaggga
      961 atcgccgatg gcctggaggc actccacccg aacgtgagga tcgatcgtga tctgctctgg
     1021 gcctgcggcc tctgccatga tctggggaaa ccctatgaga tgagccccaa gaaccaggac
     1081 cgatggaagg gtgacacggc cacccacggg tatcccgcca ttcgtcatcc ggggtatggc
     1141 atccacgtgg cgcttatggt caagctcccc gaagtcgtgg cccatactgc cgggtatcat
     1201 tccatggagg gcgaatgggt gcggcggagt ctcctcacga cgatcgtcca tcacgccgac
     1261 tacgcctact ggcacctggt ggatcgggcg ggcttgctgg tcacgcccat gcacctcaaa
     1321 taatggtgcc actgaagtgc tcggtcggtt ccagggccga tggtcccgaa gcaatgtgag
     1381 cggaacggga cgcagcgcgg tgagtcggcg ccggccgatg ctcgccggat gttggcggcc
     1441 ctacgacgcc cgcttcatcc ggtctccccg tttagcgcgt ctgatctctt acggcaccgg
     1501 aggcgccgac ccttgaaatc tcgccttctg gcggttctat tggtcttcat ctggggggca
     1561 gccgcctcgg ccgcacccgt ggctgatggg ttcgagagcg ggaccgtcgg atccctgtgg
     1621 aggacccacc gggttaagca gggcagtgcc acggttcagc aaggaatcgt ccactccggt
     1681 acgttcgccc tgcggctcga tctgcaccgg ggggatatgg ccggcctcgg tggcgacggc
     1741 gaggccacgg agcgggctga gattcaggaa gcggacgggc tgtgggcccg cttcggcgag
     1801 agctacgagt atagcttcag catgtacctg cctctggact tccccatcgt gcccacacgg
     1861 ctggtcatcg ggcagtggaa gcaggcctgt caggactgtt ctaaaaaccg cagcccgatc
     1921 atcgcccagc gctttcgtga cggcgtcttc ttcgtcacga tcgagactcc gagcgggagg
     1981 aggaccatct atcaacaccg ccaatcgatc ctgggccagt ggctggatct gcgataccgc
     2041 atccggttcg cgtggtccga cgggtcagtc acggtgtggc tgaatgacgc tcgcgtcgcc
     2101 gattaccagg gaccgctcgg ctatcccgac gacccgccgg aggtctactt caaattcggt
     2161 ttgtaccggg accggatgag ccagcccatg accatctaca tcgacgacta ccgcaaggat
     2221 cgggtcggcg agaagtagcg ctggcccccg ccggtcccc
//
LOCUS       DBNGZC010000498         3271 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1997674, whole genome shotgun sequence.
ACCESSION   DBNGZC010000498 DBNGZC010000000
VERSION     DBNGZC010000498.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1997674"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="AC16G0_08750"
     CDS             complement(<1..327)
                     /locus_tag="AC16G0_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099735.1"
                     /translation="MTNASRVPVICFLIFGVLVLAATSWSQQRPQISEQIAKTYGLDA
                     FSQIGRIRYTFNSGVGEVKLSRTWVWEPKTDQVSYEGKDKAGNPVKLTYLRSQLSSQA
                     AVVKDEI"
     gene            751..1893
                     /locus_tag="AC16G0_08755"
     CDS             751..1893
                     /locus_tag="AC16G0_08755"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5099736.1"
                     /translation="MRERPMGSILTRRIARYGVAVLVSAGALLLRQVLMASLGTRLPQ
                     YIILLPAVMLTAVLCGLGPGLLATGVAALLADYWLLPPVGFGIQDRTDAIGLGVFMAM
                     GIGMSGVAELYHRAERQLRTRSAELSRAVATLERQAGQLRSLASELTLAEQRERRRLS
                     EILHGDLQQLLIAVKLGLSPLEQAPDPRVREAYHEAMPLLGQALTVSRSLTEELSPPK
                     LHRGDLVGPLTWLARWMGEKHRLAVVVRASAGVVAESEDTASLIFQAIRELLLNVVKY
                     AHVDTAQVELARQGDQVQIVVSDAGVGFDPSQLRALGGTVGGFGLFSLGERLELLGGR
                     MEIESAPQHGSRVRLWVPLQRATAPHPSAATSGTFPPPRNAGPPSA"
     gene            2220..2909
                     /locus_tag="AC16G0_08760"
     CDS             2220..2909
                     /locus_tag="AC16G0_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005995228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HIR5099737.1"
                     /translation="MSAYPYELHLLLRLLLAVLCGAALGYERERHGRSAGLRTNLLVC
                     LGAALMMVVSKYFYYKPGEVAATIPVALDPSRIAAQIVSGIGFLGAGVILKDRGGVRG
                     LTTAATLWFNAGVGMACGAGMLLLPLVCTGIGLVSLTVMKQWEDHMPRDSYKTIALDC
                     QERTGPVLPALTQYFATRHIHVVGANCSTSAPEGATYEFVIKGKWRDLEILTHLKGLA
                     DLEQVSRVRVS"
     gene            complement(3038..>3271)
                     /locus_tag="AC16G0_08765"
     CDS             complement(3038..>3271)
                     /locus_tag="AC16G0_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008851103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5099738.1"
                     /translation="TMLAPAPLAPWITGHSIIAVQGEGPREEASDGVVAYTSAHIDGV
                     ESELVVRSGHSVQGNPEAIEEVRRILLDQAGLQ"
BASE COUNT          522 a         1076 c         1083 g          590 t
ORIGIN      
        1 gatttcgtcc ttcacgaccg cggcctgact gctgagctga gagcgcaggt acgtaagctt
       61 caccggattg cccgccttgt cttttccctc gtaggagacc tggtcggtct tgggctccca
      121 gacccacgtg cgcgagagtt tgacctcgcc cacaccggag ttgaaggtat agcggatccg
      181 cccgatttgg ctaaacgcat cgagcccata ggtcttggcg atttgttcgg agatttgcgg
      241 gcgctgctgc gaccaggaag ttgcagccag gaccagcacc ccgaaaatca gaaagcagat
      301 caccggaaca cggctcgcat tggtcatggg ttccctcctc ggtgaaaccg gataggaagt
      361 tggaaccggg gtcggccaca cgggacctca gttcgggggg caaggacggc cgcgccagcg
      421 aagcacccga gaaaacggga aatgaacacc ggattagtca tgttatcaac ggtgggtggg
      481 gctgtcaaac aggacggagt tcgaaaggca ccgataagac agaggcggcc atggtcaggc
      541 cgcaatacgg ccacacggcc ccgctcttcg gggcacgcca ccaggttcga ttggtatgat
      601 tccccgcacc ggccgcccct cgtgccttga cgccggacct gccaaggggt agcttgacat
      661 atcattcggt cgaccagggt ctggaagccc accccctcct cctgatcctg cccctttggc
      721 cgcaaaatcc ggccagattc tattcgctcg atgcgagagc gccctatggg atctatcctc
      781 acgcgccgga ttgcccgcta cggcgtcgcc gtcctggtga gcgccggcgc cctcctgctg
      841 cgccaggtgt tgatggcgtc cctcggtact cgtctaccgc agtacattat cctgctcccg
      901 gcggtgatgc tcacggcagt gctctgcggc ctggggccgg gcctcctcgc caccggcgtg
      961 gccgctctac tggccgacta ttggctgctt cccccggtag gattcggcat ccaggaccga
     1021 actgacgcca tcgggctggg ggtcttcatg gccatgggga ttggcatgag cggcgtggcc
     1081 gagttgtacc atcgggcgga acgccagctc cgcacccgga gtgccgagct gtctcgagcc
     1141 gtcgcaactt tggaacgcca ggccggccaa ctgcggtcct tggcgtccga gctgacgctc
     1201 gctgagcaac gggagcgccg gcgcctgtcc gagatattgc acggtgacct ccagcagttg
     1261 ctgattgcgg tgaagctggg cttgagcccc ttggagcagg cgccggaccc gagagtgcgg
     1321 gaggcctacc acgaggccat gcctctgctc gggcaagccc tgaccgtctc gcggtctctg
     1381 acggaagaac tcagtccccc caaactgcac cgcggcgacc tggtcgggcc gctgacctgg
     1441 ctggcccgct ggatggggga gaagcaccgg ctggccgtcg tcgtgcgggc aagcgcgggg
     1501 gtcgtggccg agagcgagga cacggccagc ctgatcttcc aggcgatccg ggaactgctg
     1561 ctgaacgtcg tgaagtatgc gcacgtggac accgcccagg tcgagctcgc gcgtcagggt
     1621 gaccaggtgc agatcgtggt ctccgacgcc ggggtgggct ttgacccgtc ccagctacgg
     1681 gcgctgggcg ggactgtggg gggttttggc ctctttagcc ttggcgaacg cttggagctc
     1741 ctggggggcc gcatggagat cgagagcgct cctcagcacg ggagccgtgt caggctttgg
     1801 gtccccctcc agcgagcgac ggcgccacac ccgtccgccg cgacgtctgg gaccttccca
     1861 cccccgcgta acgctgggcc cccctccgca tagttgcatc agcctcgaat tggacccccc
     1921 tcgacggtcc tcctgacctt tggccccagc ggtagttttt ttgggatcga ggctggcact
     1981 tcgtagtagt cgcggaattt cacagccacg ggtcgccagg ctcgcttgcc attgccacga
     2041 tgacctgcgc ggggcggagg acatcgttcc cgagccgcca gccacgtcgc acctcgcgtg
     2101 gagattcttg cggacggcag ccgggggtca ttgctcgttc ggcaggttga caattctcgg
     2161 aagtgctcta tggtctcctc atcagtaaca gtcagccgat acgccaggga gggacacaca
     2221 tgagcgccta tccatacgaa ctccacctgc tacttcggtt gctgctcgcg gtcctgtgcg
     2281 gggccgcgct cggctacgag cgcgagcgcc atggtcggag cgccgggctt cgcacgaacc
     2341 tgctcgtctg tctgggggcc gccctgatga tggtggtgtc gaagtacttc tactacaagc
     2401 cgggggaggt ggccgccacg atcccggtcg ccctcgaccc ttcccggatc gccgcccaaa
     2461 tcgtgtcggg gatcggcttc ctcggagcgg gtgtgatcct caaggaccgc gggggcgtgc
     2521 gcgggctgac cacggcggca accctctggt tcaacgcggg ggtggggatg gcctgcgggg
     2581 ccgggatgct cctcctcccg ctggtgtgca cggggatcgg gctcgtgtcc ctgacggtca
     2641 tgaagcagtg ggaggaccac atgccgcggg acagctacaa gaccatcgca ctggactgcc
     2701 aggaacggac cgggcctgtc ctgcccgccc tcacgcagta cttcgccacc cggcacatcc
     2761 acgtcgtcgg ggcgaactgc agcacctcgg ccccggaagg ggccacctat gagttcgtca
     2821 tcaagggcaa gtggcgggat ctggagatcc tcacgcactt gaaggggttg gctgatctgg
     2881 agcaggtgtc ccgtgtccgg gtgagctagg cgcctctggg gctggtccct tgcttccacg
     2941 ccgcccaggg cgcctagcga ccggcgcgac tggcgaagcg tgtccacccc tttgacaccc
     3001 acccgcccgg acgcccttcg cggccgctgc ggagttgcta ctgaagcccg gcctggtcca
     3061 gcaggatccg ccgcacctcc tcgatggcct cggggttccc ttggaccgag tgccctgagc
     3121 ggaccaccag ctccgactcc acgccgtcga tatgtgcgct ggtgtaggcc acgacgccgt
     3181 cagacgcttc ctcgcgcggc ccctcgccct gcacggcgat gatcgagtgg ccggtgatcc
     3241 agggggccag gggggccggg gccagcatcg t
//
LOCUS       DBNGZC010000499         1687 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1509965, whole genome shotgun sequence.
ACCESSION   DBNGZC010000499 DBNGZC010000000
VERSION     DBNGZC010000499.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1509965"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="AC16G0_08770"
     CDS             <1..474
                     /locus_tag="AC16G0_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099739.1"
                     /translation="TDTEFTGGHPVDGIDLLSVVAHELGHVLGYGDLDPATHQGDLMS
                     ATLVEGIRRLPEATEPSLAPRAWDPASGSLKVLDSAPPLAGVTGFALTAAPSGGTGTT
                     TTPSPANQAGSAPITWRGQDTGSPASLDPVIDEPISGPLLPSVEMQLAAKAIGKK"
     gene            567..902
                     /locus_tag="AC16G0_08775"
     CDS             567..902
                     /locus_tag="AC16G0_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011713807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HIR5099740.1"
                     /translation="MAVDKTPESAASAAESPAPTTPTIRWDDTNMKSSYANFGNVAST
                     REEFVLLFGVNQAWQAGQAEIPIQLTDRIIMSPFVAKRLALLLTNVLREYETRFGALG
                     VPGPPSTTA"
     gene            1270..>1687
                     /locus_tag="AC16G0_08780"
     CDS             1270..>1687
                     /locus_tag="AC16G0_08780"
                     /inference="COORDINATES: protein motif:HMM:NF013112.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099741.1"
                     /translation="MSQAANDSDDYRFFILDASNRCEPSAFLFTRAVNFFLLNGYRIA
                     SDIDTSAVILVNSCCVTEDKIAASMAALEVARFHAGRKRIVLFGCLASLPLPQLERED
                     LICIGPKNLDDLDSYFPHHTSIREVRSNQLPPALYEP"
BASE COUNT          305 a          562 c          523 g          297 t
ORIGIN      
        1 accgacaccg agtttaccgg cggtcacccg gtggacggca tcgatctgct tagcgtggtg
       61 gcccacgagc tcgggcacgt gctcggctac ggcgatctcg atccagcgac gcatcagggc
      121 gatctgatga gcgccacgct ggttgagggc atccgccgcc tgcccgaagc caccgagccg
      181 agccttgcgc ctcgcgcctg ggatccggcg agcggcagcc tgaaggtcct ggacagtgcc
      241 ccgcccttgg cgggcgtgac cggcttcgcc ctgaccgccg cgccctcggg cgggaccggg
      301 accactacca ccccgagtcc ggccaaccag gcgggcagcg caccgatcac gtggaggggg
      361 caggacactg ggtcaccagc gtctctcgac ccggtgattg acgagcccat cagcggccct
      421 ctcctgcctt cggtggagat gcagctcgct gccaaggcca taggcaagaa gtgatgctcc
      481 gaaggtggga tgttctgaga aggggacgcg ctgagcgacc agacacggac ccagtcagcc
      541 gcccgattct ggagcaagga ggattcatgg cagtagacaa gactcccgag tcagccgcga
      601 gtgctgcgga aagcccggct cctaccaccc ccacgattcg ctgggacgac accaacatga
      661 agagttccta cgcgaacttc ggtaacgtgg cgagcacccg cgaggagttc gtcctgctct
      721 tcggggtgaa ccaggcctgg caggcgggcc aggcggagat ccccatccag ctcaccgatc
      781 gcatcatcat gagccccttc gtggcgaagc gactggcgct cctcttgacc aacgtgctcc
      841 gggagtacga gacccgcttc ggggccttgg gtgtgccggg gcctccgagc accaccgcct
      901 agacccgaaa cctgggccct ccggacgaga ccacatctgc cgccgggtca cccccggcgg
      961 cagtgcccat ttggaacaga gctgcaagca gcagggaaag ctggcggatc cagtattgcc
     1021 agactccaga cccttccccg ttcggcagtg cttccggtgg cgagtgacct tcccctcagt
     1081 ctggggcctg accagggctt tccggttgac acgcggcacc ctcctcgatt cgctccgccc
     1141 gagagcctct cggctccaca agcgcatcac aggattgctc gcagtccggc ctcctgccag
     1201 gcgggatggg ccgcagcggc ccttgaggat aggattcatt gaccgaggcc ggtccccgag
     1261 gagcctgcct tgtcacaggc tgcgaatgat tccgacgatt accggttctt catcttggac
     1321 gcgagcaatc gttgtgagcc cagcgctttc cttttcacca gggcggtgaa cttcttcctc
     1381 ctgaacgggt accggatagc aagcgatatc gacacgagcg ccgtgattct ggtgaattcg
     1441 tgttgcgtca ccgaggacaa gatagctgcc tccatggcgg cactggaggt ggcgaggttt
     1501 catgccggtc ggaaaaggat tgttcttttc ggctgtctcg ccagtctgcc cctcccccag
     1561 ctcgagagag aagatctgat ctgcatcgga ccgaagaatc tggacgatct ggacagctac
     1621 ttcccgcatc acacttcgat ccgtgaggtc cgctccaatc agctccctcc ggcgctgtat
     1681 gagccgg
//
LOCUS       DBNGZC010000500         2140 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2293321, whole genome shotgun sequence.
ACCESSION   DBNGZC010000500 DBNGZC010000000
VERSION     DBNGZC010000500.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2293321"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..1476
                     /locus_tag="AC16G0_08785"
     CDS             286..1476
                     /locus_tag="AC16G0_08785"
                     /inference="COORDINATES: protein motif:HMM:NF024241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5099742.1"
                     /translation="MKALQVACVVCFLQFVGAYMRIPIVPLFARSEGATATEVGLIVG
                     LFMLVAAVSAIPVGRASDRWGRKRLILFGITLGGLTSLVLPALHTAGQMMLLYAVAGL
                     GVSAFTPSMISYAGDVGKPGEMGQVYGWITTAQYAGMTLGPALGGLAAGVWGYRSTFL
                     ASAAMLAMAFGTASCGLPTLPRAVPVGSPVADRHQALREVFTNRTILGCWLSTFCVTF
                     TWGVPLSFFPLYAQDAGYSAGNIGVFFAIQACCNALARVPVGRFLDRIGVRLPFILTG
                     MLGFAAATALLVTTQRWDLLALTWTLAGLSQGVGMVAVGAALGEATTRTNRGLAMGGF
                     GTAIYSGFALGSSGLGALLAHFGFRAAFTAAAGVSVAGAAACFAIMRGSKPQAKLSGG
                     KEEK"
     gene            complement(1527..>2140)
                     /locus_tag="AC16G0_08790"
     CDS             complement(1527..>2140)
                     /locus_tag="AC16G0_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_083463101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIR5099743.1"
                     /translation="VMLVGTTSEALGILALADPLRSASRQAVTDLRTAGIRHVALLTG
                     DNRAVAEAIAREVGADTVRAEVLPDQKVVTVRALRDEYGSLAMVGDGVNDAPALAASS
                     VGIAMGVAGSDVALETADVALMADDLSKLPFLMRLGRTAIRVIHQNIAFALIVKAVAI
                     AAVFPGWLTLWLAVLGDMGASVLVTLNGMRLLATKPASSKKPG"
BASE COUNT          328 a          694 c          695 g          423 t
ORIGIN      
        1 accaggtagg cctccgaccc accgtcggtg ggtacccggc gtaacggagg caatcttgtg
       61 ctgcaattca cctccctgta cactctccgc ggatgcgttg ccagattgtc tctggcgaat
      121 cgccgatggc gtatggacaa gaccgagcca aacaccatag gcaatgtgtt tgcagcgcgc
      181 atcgacccgg cttaccctga gaggggtccc accgtgggct gtgaatctgg ttggtgttgt
      241 cgaaatgttc cggctcaacc ccctgaatac tgaacccaca cccccatgaa ggcactccag
      301 gttgcctgcg ttgtgtgctt tcttcagttc gtcggggcct acatgcggat cccgatcgtt
      361 cccctcttcg cgcgtagcga aggcgcgacc gcaacagagg tgggcctcat cgtgggtctg
      421 ttcatgctcg tggcggcagt gtcggccatc cccgtcggcc gggcttcgga caggtggggg
      481 cgcaagaggc tcatcctctt tgggattacc ctgggtggcc tgacctcgct cgttctgccg
      541 gcgttgcaca ccgccgggca aatgatgctc ctctacgctg tcgcaggcct cggggtaagt
      601 gccttcaccc ccagcatgat ttcgtacgcc ggtgacgtcg gaaagcctgg cgagatgggg
      661 caggtgtacg ggtggatcac cacggcccaa tacgccggca tgacgctggg cccagccctc
      721 ggcggcctgg cggctggcgt ctggggctat cggtcaacct tcctggcttc ggcagccatg
      781 ctggcgatgg cctttggaac ggccagctgt ggtctcccga ccctgcctcg ggctgtgccc
      841 gtggggtcac cagttgctga tcggcatcaa gctctgcgtg aggtcttcac gaaccggacg
      901 atcttggggt gctggctctc gaccttctgc gtcaccttca cctggggcgt tccgctttcc
      961 ttcttcccct tgtacgccca ggatgcaggg tactcggccg gaaacatcgg cgtctttttc
     1021 gccatccaag catgctgcaa cgccctggct cgtgttcccg tcggccgctt tctggaccgg
     1081 atcggcgtcc ggctgccgtt catcctgacc ggtatgctgg gtttcgccgc ggctacggct
     1141 ctcctcgtca cgacccagcg atgggatctc ctggccttga catggaccct ggccggtctg
     1201 agccaaggag tcggcatggt agcggtggga gccgcactgg gtgaggcgac gacccggact
     1261 aaccgagggc tggccatggg tgggttcggg accgcgatct acagtggctt tgccttgggc
     1321 tcttccggtc tcggcgcact gcttgcccac tttggcttcc gcgccgcatt caccgcagcc
     1381 gcgggggtat cggttgccgg agcggccgcg tgctttgcca tcatgcgggg ctcaaaaccc
     1441 caggccaagc tgagtggagg caaggaggag aagtgaccga cgtactgaag gagagtcccc
     1501 tcgcagcttg ccgttgtctg ctcgcctcat cccggcttct tgcttgaggc aggcttggtg
     1561 gccaggagac gcatgccgtt cagggtgacg aggacggagg cacccatgtc gccgaggaca
     1621 gccagccaca aggtcagcca tccggggaag accgccgcga tggcgacggc cttgacgatg
     1681 agggcgaagg caatgttctg atggatgacc cggatggccg tgcgtcccag gcgcatgaga
     1741 aatgggagct ttgacagatc gtctgccatg agggcgacgt cggccgtctc cagggcgaca
     1801 tcgctcccag ccacgcccat ggcgattccc acgcttgagg cggccagggc cggcgcgtcg
     1861 ttcacgccat cgcccaccat ggcgaggctg ccgtattcgt cccgcaaggc ccggactgtc
     1921 accaccttct ggtccgggag cacctcggct cggacggtat cggcgcccac ctcgcgggcg
     1981 atcgcctctg ccacggcgcg gttatctccc gtcaggaggg ccacgtgccg gatcccggca
     2041 gttctgaggt cggtcaccgc ctgccggctc gccgaccgga ggggatctgc cagggcaagg
     2101 atcccgaggg cctccgaggt cgtgcccacc aacatcacgg
//
LOCUS       DBNGZC010000501         1527 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2682981, whole genome shotgun sequence.
ACCESSION   DBNGZC010000501 DBNGZC010000000
VERSION     DBNGZC010000501.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2682981"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(351..884)
                     /gene="hpt"
                     /locus_tag="AC16G0_08795"
     CDS             complement(351..884)
                     /gene="hpt"
                     /locus_tag="AC16G0_08795"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR5099744.1"
                     /translation="MTTEELGEILFSEGQIAARIKELGQAISRDYAGRDLVLAGVLRG
                     AAFFLIDLVRAITIPVAIDFIAISSYGPGSRASGVVRIVKDLEEEIAGRDLLVIEDIV
                     DTGLTLGYLLRILRERGPASLKVCTFLDRAVRRIVDPPIAYRGFEVPDKFVVGYGLDY
                     QQRYRNLPFIAVLKPPA"
     gene            complement(881..>1527)
                     /locus_tag="AC16G0_08800"
     CDS             complement(881..>1527)
                     /locus_tag="AC16G0_08800"
                     /inference="COORDINATES: protein motif:HMM:NF024587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HIR5099745.1"
                     /translation="HVQKLSDAEAETWLLEVDRQRSEWVRQHYGEDWEDPVHYDLILR
                     IDRLGLEGAAAVVRRAAEARDILGHLGDIAAWVERRGTEVSADQAGGAVGFVHPSEAE
                     FARVLDFYRIRWQYEPTSFPLAWDEAGNVTEAFRPDFYLSDLDLYLELTTLKQSLVTD
                     KNRKIRKFRELYPDRQLKVFYGRDFRSLIQKYGLPPTPSGPAAAQPHRGEGQPS"
BASE COUNT          279 a          487 c          488 g          273 t
ORIGIN      
        1 gatgcctgcg atcaccacgg tccccggcgc cagcgccgct ccacgcagaa gctcatgccg
       61 cgtcacgccc gcacgattct tcgacctgat cggggcttcc tcgcgctttg tccggtggac
      121 ggccccggag cggaacacac ggccccggcc tgagcatttg cgaagcaggc gattggacgg
      181 tatcaggtgc cgcaggctcg atctctggca gggagaattg ggcgagtcta ttcgccaccc
      241 gtgagcgctg tcaacgagat tctgaccaaa ggcgggagct ggtttcctca cgccagtgag
      301 gagtgtaccg gttgcgtgag gattttcacg ggagcacacg aatgaggcag tcaggcgggg
      361 ggcttgagca cggcgatgaa aggcaggttc cggtagcgct gctggtagtc aaggccgtac
      421 ccaacgacga acttgtcggg cacctcgaag ccgcggtacg cgatgggggg gtccacgatc
      481 cgccggacgg ctctgtcgag aaaagtgcat accttcagac tggccggccc acgttcccgg
      541 agaattctca agagatagcc cagcgtgagg ccggtgtcca cgatatcctc aatcacgagg
      601 aggtctcgcc cggctatctc ctcctcaagg tccttgacga tgcgcaccac ccccgaggcc
      661 ctgctgccgg ggccgtagct ggaaatggcg atgaagtcga tggcgacggg gatggtgatt
      721 gcccggacga ggtcaatcag gaagaaggcg gctccccgca ggaccccggc caggaccaag
      781 tcgcgaccgg cataatcgcg cgagatcgcc tgccccagtt cctttatgcg ggccgcgatc
      841 tggccctccg agaacaggat ctctcccaac tcttccgtag tcacgacggc tgcccttcac
      901 cgcgatgcgg ttgtgccgcc gctggaccgg atggagtggg gggcaggcca tacttctgga
      961 tgaggctgcg gaaatctcgg ccgtagaaga ccttcaactg gcgatcaggg taaagctccc
     1021 gaaactttcg aatttttcgg ttcttgtcgg tgacaagact ctgcttcagc gtcgtgagtt
     1081 cgagatagag atccaggtcg gagaggtaga agtccggcct gaaggcctca gtgacgttgc
     1141 cggcctcgtc ccatgccagc gggaacgatg tcggctcgta ctgccagcga atccggtaga
     1201 agtccaagac ccgggcgaac tccgcctcgc tggggtggac gaaacctaca gcgccgcccg
     1261 cttggtccgc ggagacctcc gtcccgcgtc gctcgaccca tgccgctatg tcgcccaagt
     1321 ggccgaggat gtcgcgggct tcggccgccc gtcgaaccac ggcggccgcc ccctcgaggc
     1381 ccagccgatc tatccgcagg atgaggtcgt agtggaccgg gtcctcccaa tcctctccgt
     1441 agtgctgccg aacccactcg ctacgctggc ggtccacctc cagcagccag gtctcggcct
     1501 cggcgtcact gagcttctgg acgtgct
//
LOCUS       DBNGZC010000502         2498 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1412023, whole genome shotgun sequence.
ACCESSION   DBNGZC010000502 DBNGZC010000000
VERSION     DBNGZC010000502.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1412023"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="AC16G0_08805"
     CDS             complement(<1..296)
                     /locus_tag="AC16G0_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744647.1"
                     /note="involved in the processing of the 5'end of 16S
                     rRNA; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIR5099746.1"
                     /translation="MKREIVVNSSLVETRVAVIEDGTLVEILIDDSHTQSLAGNIYKG
                     RVLKILPGMQAAFVDIGLARDAFLYVRDIYEDMELYENLLALEEEDTAEEPVDV"
     gene            complement(341..>2498)
                     /locus_tag="AC16G0_08810"
     CDS             complement(341..>2498)
                     /locus_tag="AC16G0_08810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HIR5099747.1"
                     /translation="APEPLADFIEAFLLGDGEEAVLELCAVVRAWKQSGAPREDLLRA
                     VKGIGGMYVPALHAPGETVRKRTALALDGFDYGRLPVPYMEVVHDRANIEVMRGCAQG
                     CRFCQAGYIYRPIRELSAPQIQAKVDQAVRGTGYEEISLSSLSIADLSCLGDLVPSLM
                     AALAPGKTSLSLPSLRVEALNRNKQIADEIGRVRKTGFTIAPEAGSSRLRAVINKQGF
                     DEEQIFGAVRNAAGAGWESVKLYFMIGLPTETQPDLDDLVRVARESARVARGERRRGF
                     GLTVSASSFVPKPHTPFQWFAQDPMEVLQQKQEYLKARLREARIAFKWHHVEASFLEA
                     VFALGGRELGAAIALAHERGCRFDGWTEELKFTTWMEALTAAGVNPHAIANRPRDLAA
                     PLPWDHIDCGLSRSFLEREYRKALRGAGTGDCHEGPCNNCGEICAPSWRRWTEATGST
                     PAGTPPGEGPALPEGAQDVVSGAAAAAAAKPQDVAQPIQKIRFEFHKVGDLRYLSHLE
                     LMRALQRALRRADLPLAYTQGFNPQPKISLAQALAVGVEALREPGELELTRWVDPTEL
                     LARWNRQLPPELKILRAWEAPLMGPSLSAGVRGAVYQIRLQPNGWEPSALADLGTTGA
                     CAAFLAREPIPVEASKRGRPVTLDARPFIQGFSATTEGGSPVWELNLRTGLGGSVKPQ
                     AVMRSFLGGRVPPGELDLMVSSLRVTRTALALQGQG"
BASE COUNT          398 a          860 c          832 g          408 t
ORIGIN      
        1 tccacgtcga ctggctcctc ggcggtatct tcctcttcca aggcgaggag gttctcgtac
       61 agctccatgt cctcgtagat gtcgcggacg tacaagaagg cgtcccgggc cagcccgatg
      121 tccacgaagg cggcctgcat ccctgggagg atcttcagga cgcgcccctt gtaaatgttg
      181 cccgccaggc tctgggtgtg ggagtcgtca atgaggatct cgaccagggt gccgtcctcg
      241 atgaccgcca cccgggtctc gaccaaggag gagttgacca cgatctcgcg tttcatttgg
      301 tagacctcga ccccccgcct cgggcgggga ctcgggaggc ctacccctgg ccctggaggg
      361 cgagggccgt gcgcgtgacc cgcagggacg agaccatcag atccaactca ccgggtggga
      421 ccctcccgcc cagaaagctc cgcataacgg cctggggctt cacgctgccc cccagacccg
      481 tccggaggtt gagctcccat acgggcgaac ccccctcggt tgtggcggag aatccctgga
      541 tgaagggtcg cgcgtcgagg gtcacgggac gacccctctt cgacgcctcg acgggaatgg
      601 gctcccgcgc caggaaggcg gcgcaggccc cggtggtgcc gaggtcggcc agggcggatg
      661 gctcccagcc gttcggctgc aaccggatct ggtacacagc acctcgtacc ccggcggaaa
      721 gcgacggccc catcaggggt gcttcccagg cccggagaat cttgagttcg ggcggcaatt
      781 gtcggttcca gcgagccaag agttccgtcg ggtccaccca cctggtcagc tccagctccc
      841 ccggttcccg aagagcctcg acgcccacgg ccaatgcctg ggccagggag atcttcggct
      901 gggggttgaa gccctgggta taggccaggg gcaggtccgc gcggcgcaag gctcgctgca
      961 gcgcccgcat cagttcaagg tgggaaaggt accggagatc tcccaccttg tggaattcaa
     1021 atcggatctt ctggattggc tgcgccacgt cctggggctt cgcggcggcg gcggccgccg
     1081 ccccggacac gacatcctgg gccccctctg ggagcgctgg tccctccccg ggtggcgtcc
     1141 ctgccggggt gctgccggtg gcttcggtcc accggcgcca actcggcgcg cagatctccc
     1201 cgcaattgtt gcagggaccc tcgtggcagt ccccggtccc cgcgccccgc agcgccttcc
     1261 ggtactctcg ctccaggaac gatcgcgaga gcccgcagtc gatgtgatcc cagggcaggg
     1321 gggcggccag gtcacgcggc cgattggcga tggcgtgggg gttcacgccc gcggccgtca
     1381 gcgcctccat ccaggtggtg aatttcaact cttccgtcca gccgtcgaag cggcatcccc
     1441 gctcatgggc cagggcgatg gcggcaccca gctcccggcc acccagggcg aagacggcct
     1501 ccaggaacga cgcctcgacg tggtgccact tgaaagcgat ccgtgcctcg cgcagacgcg
     1561 ccttgaggta ctcctgcttc tgctggagca cctccatggg gtcctgcgcg aaccactgga
     1621 agggcgtgtg cggcttgggg acgaaggagc tggcgctgac cgtgaggccg aagcctcgcc
     1681 gccgctcccc gcgggcgact cgcgccgatt ctcgcgccac ccgcaccaga tcgtccaggt
     1741 ccggctgcgt ctccgtggga agtccgatca tgaagtagag cttgaccgac tcccaccccg
     1801 cgccggccgc attccggacc gccccaaaga tctgctcctc gtcgaatccc tgcttgttga
     1861 tgacagcccg aagtcgggag gagccggcct cgggggcgat ggtgaacccc gtcttccgaa
     1921 ctctcccgat ctcgtcggcg atctgcttgt tccggttcag ggcctcgacc cggagagacg
     1981 ggaggctcag ggaggttttc cccggagcga gtgcggccat gagtgagggc accaggtcgc
     2041 cgaggcaaga caggtcggcg atggagagag aagacagcga gatctcctcg tagccggtcc
     2101 cacgcacggc ctgatccacc ttggcctgga tctggggggc ggacagttcg cggatcgggc
     2161 ggtagatata gccggcctga caaaaccggc acccttgggc acagccccgc atcacttcga
     2221 tgttggcccg atcgtgtacg acctccatgt agggtacagg gagccggcca tagtcgaatc
     2281 cgtccagggc cagcgcggtc cgtttgcgca cggtctcccc cggcgcgtgc agcgccggga
     2341 cgtacatccc gccgatcccc ttgaccgccc tgaggaggtc ttcgcgcggg gcgccactct
     2401 gcttccacgc gcggacaacg gcgcacaatt ccaggacggc ctcctcgccg tcgccgagga
     2461 ggaaggcctc tatgaaatcc gccaggggct cgggggca
//
LOCUS       DBNGZC010000503         3333 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_140475, whole genome shotgun sequence.
ACCESSION   DBNGZC010000503 DBNGZC010000000
VERSION     DBNGZC010000503.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_140475"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="AC16G0_08815"
     CDS             <1..304
                     /locus_tag="AC16G0_08815"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR5099748.1"
                     /translation="RVPVSRENPEVRTTTDIWRGANWHERECYDLFGVRFDGHPDHRR
                     ILLPEDWVGYPLRKDYTDERMVPYTEYGAEEKLPKAEGAKPAAKPAAAPPPKPAAS"
     gene            415..1560
                     /locus_tag="AC16G0_08820"
     CDS             415..1560
                     /locus_tag="AC16G0_08820"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HIR5099749.1"
                     /translation="MDRPVPIGTTVMLERTGESMEEMYVNMGPQHPSTHGVLRLMLKL
                     DGEAITDCIPYIGYLHRCHEKIGENRNYTQVIPYTDRLDYLASMSMNFGYVLTIERLM
                     GQQIPERAEYMRVILAELQRIASHLIWLGTFGLDLGNFTIFIYCFREREMVLDLFEAV
                     SGQRLNYSFYRIGGMPMDFPPGWVENCRAFLEWFKPRLPEYDALTADNVLFQKRLQGV
                     GVLSPDLVVDYGLSGPMIRASGIKWDLRRNDPYSIYDRFNFDIPVFDSCDVWARYMVR
                     RKEMAESVKIIEQALDQLPAGEVMAKMPKTLKPAAGQIYCRTESPRGELGYYVVSDGT
                     PKPYRYKVRSPAFVNLSALPAMLKGCLVADVVAVLGSVDIVLGEIDR"
     gene            1595..2680
                     /gene="nuoH"
                     /locus_tag="AC16G0_08825"
     CDS             1595..2680
                     /gene="nuoH"
                     /locus_tag="AC16G0_08825"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HIR5099750.1"
                     /translation="MTTLAALVDGFTATMVAWGLPLIVPTLVVMLVVAGIILAYISVA
                     AMFLIWWERKISAHIQVRYGPMRVGGWHGWAQSIADGIKLLLKEDIIPAGADKVVFVL
                     APVVVFTASLASYVTIPWAPGLIVRDLNIGVLYMVAISSLVVVGIIMAGWSSNNKYAT
                     LGAMRSAAQVVSYEVPLVLSLLGPVMIAGSMSMGKLVDAQGGTWLGILPGWFVFPQIV
                     AFLTYFICALAECNHLPFDIPEAESELVAGFHVEYSGMRFAMFFLAEYANMIVVAAIA
                     TTLFLGGWNGPWVPLPGLSRELAHGLFGLVWFQVKVYFLIFVMMWLRWTLARLRVDQL
                     MGFAWKVLLPIAFANLIVTGLVLTLKG"
     gene            2712..3203
                     /locus_tag="AC16G0_08830"
     CDS             2712..3203
                     /locus_tag="AC16G0_08830"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J family
                     protein"
                     /protein_id="HIR5099751.1"
                     /translation="MTVQLGIFYFLAAFTVWAAAVVVLGRNIVRSAVALIFTFCGMAA
                     LYVLLDAEFLAAVQVLIYVGGITILLLFAIMLTSRISVKSARVINDQVLLSAIAAVGL
                     LIGLIYAGTRGIGALVAPPRLPETSSFLGRALLTTYALPFEAVSVLLLAAMVGAIILA
                     RKE"
     gene            3212..>3333
                     /locus_tag="AC16G0_08835"
     CDS             3212..>3333
                     /locus_tag="AC16G0_08835"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit K"
                     /protein_id="HIR5099752.1"
                     /translation="MIPLSHYVILGAIIFSLGVFGALTRRNAIAILMSIELMLNA"
BASE COUNT          552 a         1075 c         1090 g          616 t
ORIGIN      
        1 tcgcgtcccg gtgagccggg agaatccgga ggtccggacg acgacggaca tctggcgggg
       61 cgcgaactgg cacgaacggg aatgctacga cctcttcggg gtccgcttcg acggacaccc
      121 ggatcaccgg cgcatcctcc tccccgagga ctgggtgggg tatccactgc ggaaagacta
      181 cacggacgag cgcatggtgc cctacaccga gtacggcgcg gaggagaagc tgcccaaggc
      241 cgagggggca aagcctgcgg caaagccggc cgcagcgcct ccaccaaagc cggcggcatc
      301 ctagcccccc accctcccct cccccagacg gggggatgag aagagtggac gcctacgcga
      361 ttcaatcgaa ggagcttcgg accatcggat ccgaagatat gaaggcggta taggatggac
      421 aggccagtac ccatcgggac gacggtcatg ctggaacgga ccggcgagag tatggaagag
      481 atgtacgtca acatggggcc gcagcacccc tccacccacg gagtgctccg cctcatgctg
      541 aagctcgacg gggaggccat caccgactgc atcccctaca tcggctacct ccatcgctgc
      601 cacgagaaga tcggcgagaa ccggaactac acccaggtca ttccctacac cgatcggctg
      661 gactacctgg cctccatgtc catgaacttc gggtatgtgc tcaccatcga gcggctgatg
      721 ggccagcaga tcccagagcg ggcagagtac atgcgggtca tcctggccga gctgcagcga
      781 atcgccagcc acctgatctg gctcggaacc tttggcctgg atctgggaaa cttcaccata
      841 ttcatctact gcttccgcga gcgggagatg gtcctggacc tgttcgaggc ggtgtccggg
      901 cagcgcctga actacagctt ctaccggatc ggcggaatgc ccatggactt cccgcccggc
      961 tgggtggaaa actgccgcgc attcctggaa tggttcaagc cccgcctccc cgagtacgat
     1021 gcgctcacag ccgacaatgt cctgttccag aaacggctcc agggggtggg ggtcctttcg
     1081 cccgacctgg tggtggacta cggtctgagc gggcccatga tccgggcgag cggcatcaag
     1141 tgggacctcc gccggaacga cccctactcc atttacgacc ggttcaattt cgacatcccg
     1201 gtgttcgata gctgcgacgt ctgggcgcgg tacatggtcc gccggaagga gatggcggag
     1261 agcgtcaaga tcatcgagca ggcgctcgac cagctgcccg cgggtgaggt catggccaag
     1321 atgcccaaga ctttgaagcc ggcggcgggc cagatctact gccggaccga gagcccccgg
     1381 ggcgagctgg ggtattacgt cgtgagcgac gggacgccca agccctaccg ctacaaggtg
     1441 cgctcccccg ccttcgtcaa tttgagcgcc ctgccggcca tgctcaaagg gtgcctcgtg
     1501 gcggacgtgg tggccgtcct gggaagcgtg gatatcgtcc tgggcgagat agaccgatga
     1561 cccctggggg tgcaccgatg gggtgtgacg cgcgatgacg acccttgccg cgctggtgga
     1621 cggcttcacc gcgacgatgg tggcctgggg gcttcccctg atcgtcccca cgcttgtggt
     1681 catgctggtc gtggccggga tcatcctggc ctacatctcc gtggccgcca tgttcctgat
     1741 ctggtgggag cgcaagatca gcgcccacat ccaggtgcgc tatgggccca tgcgggtggg
     1801 gggctggcac ggctgggccc agagcatcgc agacggcatc aagctcctgc tcaaggagga
     1861 catcatcccc gcgggggcgg acaaggtggt gttcgtcctc gcccccgtgg tggtcttcac
     1921 cgcctcattg gcgtcctacg tcaccatccc ctgggcgccg ggcctgatcg tccgggatct
     1981 gaacatcggc gtgctctaca tggtcgccat ctcctccctg gtggtggtgg ggatcatcat
     2041 ggccggctgg tcctccaaca acaaatacgc caccctgggc gccatgcgct ccgcagccca
     2101 ggtggtgagc tacgaggtcc cgctggtcct ctcgctcctg ggcccggtga tgatcgccgg
     2161 cagcatgagc atgggcaagc tggtcgacgc ccagggcggc acctggctcg gcatcctacc
     2221 gggctggttc gtgttccccc agatcgtggc cttcctcacc tacttcatct gcgccctggc
     2281 cgagtgcaac cacctgccct tcgacatccc ggaagccgag tcggaactcg tggcgggctt
     2341 ccacgtcgag tacagcggca tgcggttcgc catgttcttc ctggcggagt acgccaacat
     2401 gatcgtggtt gcggccatcg ccaccacgct cttcctgggc ggatggaacg gtccctgggt
     2461 gccgctaccc ggtctgtcgc gggagctggc ccacgggctc ttcggcctgg tctggttcca
     2521 ggtgaaggtc tacttcctga tcttcgtaat gatgtggctg cggtggaccc tggcccgcct
     2581 gcgcgtggac cagctcatgg gtttcgcctg gaaggtcctc ctgcccatcg cctttgccaa
     2641 cctgatcgtc acggggttgg tgctgacgct caaggggtag tccggaagcc tacgagaggg
     2701 gtcgggcggg gatgacggtg caactgggga ttttctattt cctcgcggcg ttcacggtgt
     2761 gggccgctgc cgtggtcgtc ctggggagaa acatcgtgcg gtcggcggtg gcgctgatct
     2821 tcaccttctg cggcatggcg gccctctacg tcctcctgga cgccgagttc ctggcggcgg
     2881 tgcaggtcct gatctacgtc ggcggaatca cgatcctgct gctgttcgcc atcatgctga
     2941 ccagccggat ctcggtcaag agcgcgcggg tgataaacga ccaggtgctc ctgagcgcca
     3001 tcgcggcggt gggtctcctg atcggcctga tctacgccgg gacgcggggc atcggcgcac
     3061 ttgtggcccc gccgcgcctc ccggagacgt cgtcgttcct cggacgggcg ctgctcacga
     3121 cgtatgcgct gcccttcgag gcggtctccg tcctgctcct ggcggccatg gtgggggcca
     3181 tcatcctggc caggaaggaa tagggagccc gatgatcccg ctgagccact acgtgatcct
     3241 gggggcgatc atcttcagcc tcggggtgtt cggggcgctg acccgccgaa acgccatcgc
     3301 catcctcatg tccatcgagc tgatgctgaa cgc
//
LOCUS       DBNGZC010000504         2098 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1605655, whole genome shotgun sequence.
ACCESSION   DBNGZC010000504 DBNGZC010000000
VERSION     DBNGZC010000504.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1605655"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /gene="cutA"
                     /locus_tag="AC16G0_08840"
     CDS             <1..325
                     /gene="cutA"
                     /locus_tag="AC16G0_08840"
                     /inference="COORDINATES: protein motif:HMM:NF015077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HIR5099753.1"
                     /translation="GLMSTYRWQGKICRDPEVLLIIKTRRRLLQTLTDRVRALHPYTV
                     PEVIALPLVGGSPAYLTWVEDTTRNPIGPGPLSGGGAPPRRQAVRRNPSPRRDAPGAK
                     GGSRR"
     gene            677..>2098
                     /locus_tag="AC16G0_08845"
     CDS             677..>2098
                     /locus_tag="AC16G0_08845"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR5099754.1"
                     /translation="MAASPPSGSPQRAQPSAALAVAVEQASTSYERGLQAMHRGDRDR
                     AEWEFDVALEALQDGNLAASGPPHLQGTALPPAHPPPDWISSLLLPQRTVVSPPGPSP
                     DEESPLEAPALLSPEDLDEVTGEKPEETTALPEPDAQKHDVPLVFNDQVRAVVYYLQN
                     RKWDVVTRAFERASRYLPMMRQTFQEEGLPLDLLNLAFIESAVNPWATSRAKAAGIWQ
                     FMPSTGRLYGMRVGWWLDERRDPEKSTRGAAQYLKKLYEMFEDWPLALAAYNAGEGAI
                     QNAIRRQRTKNFWALRLPKETRLFVPTFMAMTIIAREPERYGFSPPPEAPVEIDTVTL
                     RQPADLKVIAQAAGSTVEEIRNLNPALIRWATPPDQGHYSLRLPAGRADAFLEAFERI
                     PPDQRVSWIHHTVRKGETPAGVARRYGVDVQSVLDLNGLRKRQALKAGTKLRVPPVPT
                     PAFLVQAEKKESGRPGSYTVRRGD"
BASE COUNT          376 a          756 c          670 g          296 t
ORIGIN      
        1 gggcctgatg tccacctatc gatggcaggg caagatctgc cgggaccccg aggtcttgct
       61 gatcatcaag acgcgccgcc ggctgctcca gaccctgacg gatcgcgtcc gggccctgca
      121 cccgtacacg gtacccgaag tcatcgccct gcccctggtc gggggctcgc ccgcttacct
      181 cacctgggtg gaggacacca cccggaatcc catcggccct ggtcctctgt ctggcggggg
      241 cgcgcccccg cgaaggcagg cagtccgaag gaacccctcg ccccgccggg atgcccctgg
      301 ggcgaaaggg ggctcgaggc ggtgaacggc tgcacgtcga cgaggaaggg aaaagggcat
      361 ccgccgatcc aaccccacga aaatcttgac aaacagggag accgcgggta agaaacatta
      421 gaagagtcgc gcccgcttcg acaggggccg ggcagcttga ccgaaagaca tcatcactca
      481 aggatgcttg atgccccttc ggcaaccctc tcgacctctg gttgtcgttt ggctgcttgt
      541 cttcctgacc ggatgcggca cggccggcac gctaccccac cccacacccc aagccagtgc
      601 ggatcctcca ccggccgacg agctgtggag cgggccgaag acgcctaagc tggcgacccc
      661 ggtgccggtc gttcccgtgg cagcgtcacc cccctctggt tcccctcaac gcgcccagcc
      721 ctccgctgcc ctggcggtcg ccgtggagca agcgagcacc tcctatgagc gagggctcca
      781 ggccatgcac cgcggggacc gggaccgggc cgaatgggag ttcgacgttg ccctggaggc
      841 cctgcaggac gggaatctcg ccgcaagcgg gccgccccac ctccagggca ccgccctgcc
      901 gccggcacac cctccgccgg actggatcag ctccctgctc ctgccgcaac ggacagtggt
      961 gtcgccgccc gggccatcac ccgacgaaga gtcgccgctc gaggcgcccg cgctgctcag
     1021 ccccgaggac ctcgacgagg taaccggcga gaagccggaa gagaccaccg ccctcccaga
     1081 gcctgacgcc cagaagcacg atgtccccct cgtcttcaac gaccaagtgc gggcagtggt
     1141 ctactatctt cagaatcgga agtgggacgt cgtcacgcgt gccttcgagc gggccagtcg
     1201 ctatctgccg atgatgcgac agaccttcca ggaggaaggc ctccccctcg accttctcaa
     1261 cctggcgttc atcgaaagcg ccgtgaatcc ttgggccacg tcccgggcta aggcggccgg
     1321 catctggcag ttcatgccgt cgaccggacg gctgtacggc atgcgggtgg gctggtggct
     1381 ggacgagcgg cgagatccgg agaaatcgac ccggggggcc gcgcagtacc tcaagaaact
     1441 ctacgagatg ttcgaggact ggcccctcgc cctggccgcc tacaacgcgg gcgaaggggc
     1501 gatccagaac gccatccggc gccagaggac caagaacttc tgggcgctcc ggctccccaa
     1561 ggagacccgg ctcttcgttc ccacgttcat ggccatgacc atcatcgccc gggagccaga
     1621 gcgctacggc ttctcgcccc cgccggaggc gccggtcgaa atcgacacgg tcaccctgcg
     1681 tcagccggcc gatctgaagg tcatcgccca ggccgccggc agcacggtcg aggagatccg
     1741 caacctgaac cctgcgctga tccgctgggc gactccgcct gaccagggcc attacagcct
     1801 gcgcctcccg gccggccggg ccgacgcgtt cctcgaggcg ttcgagcgca tcccgccgga
     1861 ccagcgggtc agctggatcc accacacggt gcgcaagggt gagacaccgg caggcgtcgc
     1921 ccggcggtac ggcgtcgatg tgcaaagcgt cctcgacctc aacggcctcc gcaagcggca
     1981 ggccctgaaa gcgggaacca agctccgggt cccgccggtg ccgaccccgg cattcctggt
     2041 tcaggccgag aaaaaagaga gcgggcggcc ggggtcatat acagtccggc gaggcgat
//
LOCUS       DBNGZC010000505         2791 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2683178, whole genome shotgun sequence.
ACCESSION   DBNGZC010000505 DBNGZC010000000
VERSION     DBNGZC010000505.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2683178"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..410
                     /locus_tag="AC16G0_08850"
     CDS             72..410
                     /locus_tag="AC16G0_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018281349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR5099755.1"
                     /translation="MEIQVRNHTGAAIIEVRGEVDLYTSPQVREAILGAMSARTPTVV
                     VDLSGVGYMDSSGVATLVEGLQSSRAYGGAFRLAGLGDTVRQVFKFARLEKVFEIYLN
                     AQEALARPRA"
     gene            460..1254
                     /locus_tag="AC16G0_08855"
     CDS             460..1254
                     /locus_tag="AC16G0_08855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family ABC transporter permease"
                     /protein_id="HIR5099756.1"
                     /translation="MAEVLAGMGRRALRVRDDLGAAARLLVAMAARLGSAPFSRKGFR
                     WPATFRQMVRIGVDSLPIVFLIAGSVGMIVALQAATQLRRVGATIYVADLVGISLTRE
                     LGPLMTAIIIAGRSGSAIAAELGSMKVAEEVDALTAMGLDPVEFLVLPRILAMGIMLP
                     CLTVLADVIGILGGVVVAGVSLGIPAGNYLEQTLNALLLKDLFSGLVKSWVFAGIIAG
                     VGCYQGFRVEGGAEGVGRRTTASVVVSIFLIVAADLVFTLAFYVLG"
     gene            1334..2131
                     /locus_tag="AC16G0_08860"
     CDS             1334..2131
                     /locus_tag="AC16G0_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099757.1"
                     /translation="MSTPNAIIEVHDLVTQYGARRILDGASLRVPEGLITVILGGSGS
                     GKSTLLRHLIGLQKPTGGQILVKGRDLAPMTPDELVEMRTRMGILFQGGALFNSMTVG
                     ENVALPLREHTRLEESTIQIMTRIKLQLVGLAGFEDFMPAQLSGGMKKRAGLARAIAM
                     DPEILFFDEPSAGLDPLTAAALDELILKLKQTFRMTILVVTHELPSVFAIADHVVMLM
                     NGKVLMEGSLDELRASPDPRIQDFLARRPAPETEDAEAYLRSLVGTS"
     gene            2141..>2791
                     /locus_tag="AC16G0_08865"
     CDS             2141..>2791
                     /locus_tag="AC16G0_08865"
                     /inference="COORDINATES: protein motif:HMM:NF014523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIR5099758.1"
                     /translation="MATRDEVKAGIVIAGSLLILAALVIGVSGLSLWERYDRYTVRLH
                     AIAGLDQGSPVRLGGLKVGRILGLRIPPDDTARVEITLGVRQGTHIPQGSWASVATLG
                     LLGDTFLQLTTERHSSQRIPPGSEIPSREVAGIADLVQHLQRVADSAETVLSEVATVL
                     TRDVADLMRRVNGLADTTQKTVTHIDAFVAPANRERVERMLATLDQAVQEGTASIRV"
BASE COUNT          443 a          868 c          995 g          485 t
ORIGIN      
        1 cgtgtcggca gatgggccgg ggatggaact caccctggtg aagcatctgc gcgggggagc
       61 ggggaggcca agtggagatc caggtccgta atcatacagg cgcggcgatc atcgaggtgc
      121 ggggcgaggt ggacctgtac acctcccccc aggtgcgaga ggcaatcctc ggggccatgt
      181 cggctcggac gccgaccgtg gtggtggatc tttccggcgt gggctacatg gacagctccg
      241 gcgtggccac gttggtggag ggtctgcagt cctcccgggc gtacggcggc gcctttcggc
      301 tggccggcct cggagacacg gtccgccaag tcttcaagtt cgccaggctg gagaaggtgt
      361 tcgagatcta cttgaatgct caggaggcac tggcccggcc gagggcctag cgcatgcctg
      421 gtgatgcaag gctgatggcg aaggctcgag ggcggcgaca tggctgaagt cttggcggga
      481 atgggtcgcc gagcgctccg cgtccgcgac gacctcggcg ccgccgcacg gctcctggtc
      541 gccatggcgg cccgtctcgg ctccgccccc ttcagccgca aggggttccg gtggcccgcg
      601 accttccgcc agatggttcg gatcggcgtg gattctctcc ccatagtttt tcttatcgcg
      661 ggatcggtgg gaatgatcgt cgcgctccag gcggccacgc agttgcgacg ggtgggcgcc
      721 accatctacg tggccgatct ggtggggatc tccctgaccc gcgagctggg accgctgatg
      781 acggccatca tcatcgcggg caggagcggc tccgccatcg ccgccgagct ggggagcatg
      841 aaggtggctg aagaggtgga cgccctcact gccatggggc tggacccggt ggaattcctg
      901 gttctccccc gtatcctggc catggggatc atgctcccct gcctgacggt cctggcggac
      961 gtgatcggga tcctgggcgg ggtcgtggtg gcgggggtga gcctggggat tcccgccggg
     1021 aattacctcg agcagacgtt gaatgccctc ctgctcaagg atctcttcag cgggctggtg
     1081 aagagctggg tcttcgccgg gatcatcgcc ggcgtgggct gctaccaggg ctttcgcgtg
     1141 gagggaggtg ccgagggggt gggcagacgc accacggcct cggtggtggt gtccatcttc
     1201 ctgatcgtcg ccgcggacct ggtgttcacg ctggccttct acgtcctggg ttgaggtgtg
     1261 gaggttcaag cgggcacgcg tccgagcctt cggacgttgt cgagccgcag caagctctgg
     1321 ccggtagcgc gagatgtcca ccccgaatgc cataatcgag gtccatgacc tggtgactca
     1381 gtacggcgcc cgccggatcc tggacggcgc gagcctccgg gtgccggaag gcctcatcac
     1441 ggtgatcctg ggagggagcg gctccgggaa gagcacgctg ctgcgtcatc tcatcggcct
     1501 gcagaaaccc acgggcggac agattctggt gaagggccgg gacctcgcgc ccatgacacc
     1561 cgacgagctg gtggagatgc ggacgcgcat gggcatcctg ttccagggcg gggccctctt
     1621 caactccatg accgtggggg aaaacgtggc gctgcccttg cgagagcaca cacggctgga
     1681 ggaatccacg atccagatca tgacccggat caagttgcag ctcgtgggtc tggctgggtt
     1741 cgaggacttc atgccggccc agctctccgg cggcatgaaa aagcgggccg ggctggcacg
     1801 ggccatcgcc atggaccccg agatcctgtt cttcgacgag ccgtcggcgg gcctggatcc
     1861 cctcaccgcg gcggcgctgg acgagttgat cctgaagctc aagcagacct ttcgcatgac
     1921 gatcctggtg gtgacccacg agcttcccag cgtctttgcc attgcagatc atgtggtcat
     1981 gctcatgaac ggcaaggtcc tcatggaggg aagcctggat gaactccggg cgtcaccgga
     2041 tccgcgcatc caggacttcc tggccaggcg gcccgcgcca gagaccgagg atgccgaggc
     2101 ataccttcga tccctggtgg gcacctcgtg atggaggcac atggccacgc gggatgaggt
     2161 gaaggcgggc atcgtcatcg ccgggtcgct gctgatcctg gcagccctgg ttatcggcgt
     2221 gagcgggctg agcctgtggg aacgctacga ccggtacacc gtccggctgc acgccatcgc
     2281 cggcctggat cagggatcgc cggtgcgact cggcggcttg aaggtgggcc gcatcctcgg
     2341 cctgcgcatc ccgcccgacg acacggcccg ggtggagatt accctgggcg tgcgtcaggg
     2401 gacccacatc ccgcagggct cgtgggccag cgtggcgacc ttggggctct tgggggatac
     2461 gttcctgcag ctcaccacgg agcgacacag cagccagcgg attccccctg ggagtgagat
     2521 cccgagccga gaggtcgccg gcatcgccga tctcgtccag cacctgcagc gagtggcgga
     2581 ctcggccgag accgttcttt ccgaggtggc gacggtcctg acccgagacg tggcggatct
     2641 gatgcgacgg gtgaacggcc tcgccgacac cacccagaag accgtgaccc acatcgacgc
     2701 cttcgtcgcc ccggccaatc gcgagcgggt ggaaaggatg ctggccacgc tggaccaggc
     2761 agtccaggag ggaaccgcct ccattcgcgt g
//
LOCUS       DBNGZC010000506         4341 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2486621, whole genome shotgun sequence.
ACCESSION   DBNGZC010000506 DBNGZC010000000
VERSION     DBNGZC010000506.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4341)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2486621"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="AC16G0_08870"
     CDS             complement(<1..628)
                     /locus_tag="AC16G0_08870"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009093657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR5099759.1"
                     /translation="MEPRQESRSETPTLSRNGEVGELVRRLVEAAGGSAHADFLQEMI
                     GSVLRLIEDQADRGDVKILNAALRELVHAFKIFAPYRGIRKVTMFGSARTAEDQPEYL
                     QAQAFAQAISRQGWMVITGAGDGIMKAAQGGAGRERSFGVNIRLPFEQVANEYIQNDF
                     KLITFRYFFTRKLIFVKETDAIVLFPGGFGTMDEAFETLTLMQTGKSNL"
     gene            complement(651..1598)
                     /locus_tag="AC16G0_08875"
     CDS             complement(651..1598)
                     /locus_tag="AC16G0_08875"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR5099760.1"
                     /translation="MGRAEIAPGRRRVLLDVDTGVDDALALMLAIRSPELEVLGITTV
                     SGNVPVARSTANTLLVLEILDAPAIPVVAGAPAPLARAPVTATQVHGADGLGNISAAY
                     PVPKRSAGEGAVEFLLETICRFPGELTLIATGPLTNVAMALQRDGATMRQLRGLTVMG
                     GAVRVPGNLGPVTEFNFGVDPEAAAIVFRSGLNPTLVPLDVTHQVVLSRKAVDGTEGR
                     LRDFIQRMTAPAMAFHQEHEGFDGIFLHDPLAVGIVVDPSLARGQALPLAVECRGELT
                     AGMAVADLRRRSRACPTATVCVEVAAHRFLELFQRRVLN"
     gene            complement(1761..2006)
                     /locus_tag="AC16G0_08880"
     CDS             complement(1761..2006)
                     /locus_tag="AC16G0_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099761.1"
                     /translation="MDEPLAQHILDIIYQDSDFRRLYKESLTDWILDTQPRTAPLDAA
                     ALLQFLSTHQPDLLNRLKINVRIKEELARALESIEQN"
     gene            complement(2082..2822)
                     /locus_tag="AC16G0_08885"
     CDS             complement(2082..2822)
                     /locus_tag="AC16G0_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR5099762.1"
                     /translation="MDAFKRLKAQVIACRLCPRLVAHRERTAREKVRRYRGGEYWGKP
                     IPGFGDPRAGLLIVGLAPAAHGGNRTGRIFTGDRSGDFLFAALHRTGFANQPWSRHLD
                     DGLRLRDCYITAAVRCAPPENKPTPLELATCRHYLVEELRLLSRVRVVVVLGRIAFEA
                     FLKAWVEAGGKLPARRPQFGHGLMTELPGGLRLLASYHPSQQNTQTGRLTVPMFQRIF
                     AQARRLLTDPKAAQAQTGGRQKGEKSEQ"
     gene            complement(2854..3258)
                     /locus_tag="AC16G0_08890"
     CDS             complement(2854..3258)
                     /locus_tag="AC16G0_08890"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR5099763.1"
                     /translation="MRLKKAEREFVAFQRVCRVATVNRKGEPHNVPVCPILDGDRVYF
                     ASEAGAVKVRNVRSTGHVALTFDEYTEAWSGLKGVLIRGMGRTIEAGPAFRRVRKLLY
                     QRFPQYEGYASLEEKNTVIVEVTPTRTFSWGL"
     gene            complement(3331..3528)
                     /locus_tag="AC16G0_08895"
     CDS             complement(3331..3528)
                     /locus_tag="AC16G0_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099764.1"
                     /translation="MIRDRIVELRAKIRLTKNEIAHLWNGGILSLKIEGPGVEADLEL
                     RCPETEPDRGTRILIKRVSAE"
     gene            complement(3736..>4341)
                     /locus_tag="AC16G0_08900"
     CDS             complement(3736..>4341)
                     /locus_tag="AC16G0_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099765.1"
                     /translation="LFPIVEASASLSFLAQSSQQTSTAPLARGRVGFLAVSDGGRSAT
                     IMVGPVHGPEHLEVIRTYRFYHVPASSIAASRLTVPYIAFYEGASRFKLETGVIREYA
                     EVLKVTRVRRCDLPGLTWPGRHGEEASYYRFDLGPLVTLPHPITNPDQLRVVFRFPDI
                     QKFRRAETLHDLGDRPLQGQEGGAGARAPKTVRGRKGAGKT"
BASE COUNT          778 a         1400 c         1401 g          762 t
ORIGIN      
        1 ggaggttgct ctttccggtc tgcatcagcg tcaaggtctc gaaggcctcg tccatggttc
       61 cgaagccgcc gggaaagagg acgatggcgt cggtctcttt cacgaagatc agcttccggg
      121 tgaagaagta ccggaacgtg atgagcttga agtcgttctg gatgtactcg ttggccacct
      181 gctcgaaggg caggcggatg ttcacgccga agctccgctc ccgtccggcg ccgccctggg
      241 cggctttcat gatgccgtca cccgcgcccg tgatgaccat ccagccctgg cgactgattg
      301 cctgggcgaa ggcctgagcc tgcaggtatt ccggctggtc ctccgccgtc cgggccgagc
      361 cgaacatcgt caccttccgg atgccccgat agggggcgaa gatcttgaag gcgtgcacca
      421 gttcgcgcag ggcggcattc aagatcttca cgtccccccg atccgcctgg tcctcgatga
      481 ggcgaaggac gctcccgatc atctcctgaa ggaagtcggc gtgtgccgac ccgccagccg
      541 cctccacgag tctgcgcacc agctccccga cctcgccatt ccgagagagc gtcggggtct
      601 ccgagcggga ctcctgtctt ggttccaagt tccacctcgc gagctttgac tcagttcagg
      661 acacggcgct ggaacaactc caaaaatcgg tgggccgcga cctcgacgca gactgtggcc
      721 gtcgggcagg cccgactgcg ccgccgaaga tcggcgaccg ccatcccggc ggtcagctcc
      781 ccccggcact cgaccgctaa cggaagcgcc tggccccgag ccaaggaggg atcgactacg
      841 atccccacgg ccaaggggtc gtggaggaat atgccgtcga agccctcgtg ctcttggtgg
      901 aaggccatgg ccggggcggt catcctctgg atgaagtccc ggagcctccc ctcggtcccg
      961 tccacggcct ttcgggagag gaccacttgg tgcgtgacat ccaggggaac caaggtcggg
     1021 ttgagccccg aacggaagac gatcgccgcc gcttccgggt ccacgccgaa gttgaattcc
     1081 gtcacggggc caagattgcc cgggacccgg acggcccccc ccatgacggt gagcccccgg
     1141 agttggcgca tcgttgcgcc atcccgctgg agcgccatgg cgacattcgt gagcggcccc
     1201 gtggcgatga gcgtcaattc gccggggaaa cggcagatcg tctccaggag gaattccaca
     1261 gcaccctcgc cggcgctccg cttcggcacc ggataggcgg cgctgatgtt gccgagcccg
     1321 tccgccccat gcacttgggt cgccgtgact ggcgcccggg cgagaggcgc aggagccccg
     1381 gccaccaccg ggatcgcggg agcgtcgaga atctccaaga ccagaagggt attggccgtg
     1441 gagcgggcca ccgggacgtt cccactcacc gtggtgatgc ccaggacttc tagctccggc
     1501 gagcgaattg ccagcatgag agccagtgca tcatccacgc cggtgtccac atccagaagg
     1561 acccgccgcc gccctggggc gatctcagcc cttcccatga ctactccttg ggttccagga
     1621 atcgcgacgg atgcgcccgc tgaaaatatg ccttccccca agagacagcg ggggcgatca
     1681 cgccgagaaa acagggcgtt gaaccagcca gcgatacggc gtgggaacgt caggcgggag
     1741 ggaaggtgcc gcgctgaaga ctagttttgc tcgatggatt ccaacgcgcg ggccagttcc
     1801 tctttgatcc tcacgttgat cttcagccga ttgaggagat cgggctggtg tgtcgacagg
     1861 aattgcagca gggctgccgc atccaacggg gccgtgcggg gctgtgtatc gagtatccag
     1921 tccgtaaggg actccttgta gagacgtcga aagtcggagt cctgatagat gatgtcgaga
     1981 atgtgttggg ctaagggttc gtccacagaa tcctcctcat ggacctctca atctgaagga
     2041 aatatagcag acaaatttgg ctctgacaag accacacttg atcattgttc acttttttca
     2101 cccttttgcc gccctcccgt ttgcgcctgg gcggctttcg ggtcggtcaa caggcggcgg
     2161 gcttgggcga agatccgctg aaacatcgga acggttaggc gcccggtttg cgtgttctgc
     2221 tggctcgggt gataggacgc gagcaagcgc aggcctccag gtaactccgt catcaggccg
     2281 tgaccgaatt ggggtcgccg ggcaggcaac ttcccaccgg cctccaccca cgccttgaga
     2341 aaggcctcaa aggcgatccg gccgagaacc acgacgaccc gcactcggga caagagtcga
     2401 agctcctcca ccaggtagtg ccggcaggtc gcaagctcca atggcgtggg tttgttttcc
     2461 gggggtgcgc aacggacggc ggcggtgatg tagcaatctc ggagacggag gccgtcgtcc
     2521 aggtgccgcg accatggctg gttggcaaag ccggtccggt gcaaggcagc gaataggaaa
     2581 tccccgctcc gatcgccggt gaagatgcgc ccggtacggt tgccgccgtg ggccgcgggc
     2641 gccaaaccga cgatcaatag accggccctg ggatcgccga accccgggat gggttttccc
     2701 cagtactccc cgccgcgata gcgccgcacc ttctcccgcg ccgtgcgctc ccggtgtgca
     2761 accaggcgcg ggcacaggcg acacgcgatc acctgtgcct tcaagcgttt gaacgcatcc
     2821 atccttggcg acttggcggg ttcggattgg acttcacagg ccccaactga acgtccgggt
     2881 cggtgtgacc tcgacgatca ccgtgttctt ctcttccagc gacgcgtacc cctcgtactg
     2941 cgggaagcgc tggtagagca gttttcgaac ccggcgaaat gccggcccgg cctcgatcgt
     3001 ccggcccatc ccccggatca ggacgccctt caggccagac caggcctcgg tgtactcgtc
     3061 gaaggtcagg gcgacgtgac ccgtgctccg gacgttccga accttgacgg cgcctgcttc
     3121 agaggcgaag tagactcgat ccccgtcgag gatcgggcag acggggacgt tgtggggttc
     3181 ccccttccgg ttgaccgtgg ccacccggca gacccgctga aatgccacaa actcccgctc
     3241 ggccttcttc agtcgcatgt cgggctcctc ggctcggcgc ccaggatcag tagagaaggg
     3301 atcgatccgg cggcgacggc ggtgcgagcg tcattccgcg ctcacgcgct tgatgagaat
     3361 gcgagtgccc ctgtcgggct cggtctcggg acagcggagt tcgaggtccg cctcgacacc
     3421 ggggccctcg atcttgaggg agaggattcc tccgttccac aggtgcgcga tctcgttctt
     3481 cgtgaggcga atcttcgccc gaagttctac tatgcggtct ctaatcatgg tgacagtcca
     3541 cctccaggac cgcagagtcc gggggagagt ccgaggaggg gtggcggccc acccctctcg
     3601 gaagggtccg cggggagggc gagcgattgc ccgtcccggt gcaagcgcgc gggccgtccc
     3661 cgcgaactaa gcggtccggg cggatatgta gcctaccttc cggaccgctt agtaaaggcg
     3721 aaccaacgtt ctgcgtcatg tcttgcccgc ccccttccgc cccctgaccg tcttcggtgc
     3781 tctcgcgccg gcccctccct cttgaccctg gagcgggcga tccccaagat cgtgcagggt
     3841 ctcggccctg cgaaatttct ggatgtccgg aaaccggaac actacccgaa gctggtcagg
     3901 gtttgtgatc gggtgaggga gagtcaccag gggccccagg tcgaaacggt agtaggacgc
     3961 ctcttcccca tgccgccccg gccaggtcaa gcctggcagg tcgcaccggc gaacgcgggt
     4021 gaccttcagg acctcggcgt actcccgaat cacgcccgtc tcgagcttga accgggaagc
     4081 gccctcgtag aaagcgatgt aggggaccgt gagtcgagag gcggcgatgg aggaggcggg
     4141 cacatggtag aaccggtagg tgcggatgac ctccaggtgc tccggcccgt ggaccggccc
     4201 taccatgatt gtcgcactgc gcccgccgtc cgacaccgcg agaaaaccca cgcgcccgcg
     4261 ggccaggggg gccgtcgagg tctgctgtga ggactgcgcc aaaaaactga gggaagctga
     4321 tgcttccaca atgggaaaga g
//
LOCUS       DBNGZC010000507         1924 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_43513, whole genome shotgun sequence.
ACCESSION   DBNGZC010000507 DBNGZC010000000
VERSION     DBNGZC010000507.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_43513"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="AC16G0_08905"
     CDS             <1..481
                     /locus_tag="AC16G0_08905"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR5099766.1"
                     /translation="DGILVNDDWGTQETLMIRPEYWRKIYKPAYAKIVQAIHSGGCFA
                     HLHTDGVTEAIMEDLIEIGFDEINPQMYCKDIEDLGKRYWGRVCFRADMDRQYTLPFG
                     TPAEVETYVQRMYAAFGGFNGGYVGYGQVNTDVPLENAEAMLAAFSQLRYAPPGAKD"
     gene            492..1616
                     /locus_tag="AC16G0_08910"
     CDS             492..1616
                     /locus_tag="AC16G0_08910"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR5099767.1"
                     /translation="MTNRERILAVLDGRSPDRIPWIARLPLWYHARLTEGSLPARFEG
                     MDLLEIARTLRTGNPARDGRVFQVRYEGLDVQVEREPGVLRKRFVTPYGEVTYGLTLT
                     GELEGRTDQGLPLDHPIKGVRDYRVWEYVAEHTFYDPCHDEYLAYEAAVGEEGYPMVN
                     AGDCPFHYFLLHLCGYNTAYYEMVDHPGEFEHLLTVMTQVEKERLWPVVANSPARLIL
                     HGAHFDSQMTPPHMFRKYITPYYQEFSRLLHARGKRLTWHADDDTRDLLGEIQQAGFD
                     MSECFCTAPMVSVTLEEARAAWGREVIIFGGVPSIVLEATFPEAEFEEYMRGVFRAIA
                     PGDAFILGVADNVMPTSLIQRVERISELVEQHGTYPVQTY"
     gene            1691..>1924
                     /locus_tag="AC16G0_08915"
     CDS             1691..>1924
                     /locus_tag="AC16G0_08915"
                     /inference="COORDINATES: protein motif:HMM:NF014646.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HIR5099768.1"
                     /translation="MANYDALKTAVIEGKMAQVKTLTQQALDEGKGPQEILDQALIPA
                     MDVVGEKFNCHEFYIPEMLISARAMQAGVAILKP"
BASE COUNT          367 a          592 c          645 g          320 t
ORIGIN      
        1 cgacggaatc ctggtgaacg acgactgggg gacgcaagag accctgatga tccggcccga
       61 gtactggcgc aagatctaca aaccggccta cgccaagatc gtccaggcga tccacagcgg
      121 ggggtgcttc gcccacttgc acacggacgg agtgaccgag gccatcatgg aggatctgat
      181 cgagatcggc ttcgacgaga tcaaccccca gatgtactgc aaggacatcg aggatctggg
      241 gaagcgatac tggggtcggg tgtgcttccg cgcagatatg gatcggcagt acaccctgcc
      301 tttcgggacc ccggccgagg tcgagaccta cgtgcagcgg atgtacgctg ccttcggcgg
      361 attcaacggc ggatatgtgg gttacggcca ggtcaacacc gacgtccccc tggagaacgc
      421 cgaggccatg ctggcggcct tttcccaatt aaggtatgct ccgcccgggg cgaaggactg
      481 agggtgggcc gatgaccaac cgcgagcgta tcctggccgt tctggacggc cggagccccg
      541 accgcatccc ctggatcgcg cggcttcctc tctggtacca cgcgcgcctc accgagggca
      601 gtctaccggc ccgcttcgag ggaatggacc tgctggagat cgcccgcacc ctgcgcacgg
      661 gcaatccggc ccgggatgga cgggtcttcc aggttcggta cgaggggctg gacgtccagg
      721 tggagcggga gcccggcgtc ctgcgcaagc gcttcgtcac gccgtacggt gaggtcacct
      781 acggcttgac cctgaccgga gaactggagg gtcgcaccga ccagggccta ccgctcgatc
      841 accccatcaa gggcgtaagg gactaccggg tctgggagta cgtggcggag cacaccttct
      901 acgatccctg ccatgatgag tatctggcgt acgaggcagc ggtgggagag gaaggctatc
      961 ccatggtgaa cgccggggat tgccccttcc actacttcct gctacacctg tgcgggtata
     1021 acaccgccta ctacgagatg gtggatcatc ccggcgagtt cgagcacctc ctcacggtga
     1081 tgacccaggt ggagaaggag cggctgtggc cggtggtggc gaactcaccg gcgcgcctga
     1141 tcctgcacgg cgcccacttc gattcgcaga tgacaccgcc ccacatgttc cggaagtaca
     1201 tcacgccgta ctatcaggag ttcagccggc tgctgcacgc gcggggcaag cgcctgacct
     1261 ggcacgcgga cgacgacacc cgggatctgc tcggcgagat ccagcaggcc ggcttcgaca
     1321 tgtccgagtg cttctgcacg gcccccatgg tgagcgtgac cctggaggag gcaagggccg
     1381 cctggggtcg ggaggtcatc atcttcggcg gcgtgccctc catcgtgctg gaggcgacct
     1441 tccccgaggc ggagttcgag gagtacatgc ggggagtgtt ccgggccatc gcgccgggag
     1501 atgccttcat cctgggcgtg gccgacaacg tcatgccgac gtcgctcatc cagcgggtgg
     1561 agcggatctc cgagctggtg gagcagcacg ggacctatcc cgttcagacc tactgaattg
     1621 agttcgcggg gagcgtccga gcgcaacgga gccactggcg cttaatgacc ggggaaccga
     1681 ggaggagacg atggccaact acgacgcctt gaagaccgcc gtgatcgagg ggaagatggc
     1741 ccaggtgaag accttgaccc agcaggctct ggacgaaggc aagggtcctc aagagatcct
     1801 ggaccaggcc ctgattcccg ccatggatgt cgtgggggag aaattcaact gccacgagtt
     1861 ctacattccc gagatgctga tctccgcccg ggccatgcag gcgggggtgg ccatcctgaa
     1921 accg
//
LOCUS       DBNGZC010000508         3761 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1903751, whole genome shotgun sequence.
ACCESSION   DBNGZC010000508 DBNGZC010000000
VERSION     DBNGZC010000508.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1903751"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            357..1463
                     /gene="gcvT"
                     /locus_tag="AC16G0_08920"
     CDS             357..1463
                     /gene="gcvT"
                     /locus_tag="AC16G0_08920"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR5099769.1"
                     /translation="MSGPEGPLKQTPLNAVHHQMGAKMVPFGGWDMPVQYRGILDEHR
                     AVRSAAGLFDVSHMGEVEFRGPAALEAVQRLTTNDASRLKVGQVQYTALTTEAGTFVD
                     DLTVYKFADDHYMVTVNASNIDKDVAWMRSHTTGSVEVRNASDETALLAIQGPRALAI
                     LQKLTPVDLGAIPYYWFAQGKVLGVDAVLSRTGYTGEDGFEVYVKPKDVVALWQALLE
                     AGTTQGLLPCGLGARDTLRLEAKMALYGNDIDDRHTVLEADLGWILKLQKGEFIGRAA
                     LAKQKEEGVRRKLVGFEMIGRGIGRSHYPIVKDGRPIGEVTSGGPAPWLNKNIGLGYV
                     AQEHAAVGTDIEILIRGTPVTARIVQTPFYKRPR"
     gene            1593..1982
                     /gene="gcvH"
                     /locus_tag="AC16G0_08925"
     CDS             1593..1982
                     /gene="gcvH"
                     /locus_tag="AC16G0_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR5099770.1"
                     /translation="MYPADLKYTKEHEWIKVERGLGRVGITHHAQNALGDVVFVELPK
                     VGRAVKQMETFGVVESVKAVSDLYSPASGEVVEANGALEGKPELVNQDCYGAGWMIVV
                     KLANPKELDALMDAKAYEAYLAAEGGH"
     gene            1982..3328
                     /gene="gcvPA"
                     /locus_tag="AC16G0_08930"
     CDS             1982..3328
                     /gene="gcvPA"
                     /locus_tag="AC16G0_08930"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HIR5099771.1"
                     /translation="MRYIPNTDLDRRQMLEAIGVGSVLDLFADVPSEVRLKRSLKVPP
                     ALSEAELGKHLRTLAGKNADADRFSTFLGGGCYHHFSPAIINHLVLRGEFLTAYTPYQ
                     PEISQGTLQALYEYQTLICLLTGMEVSNASMYEGASSTAEAILMAQRVTGRNEVVVAR
                     AVHPEYRQVARTYTAQVGITFKEVPFAEAGHTDAGIVQQALSEHTACLVIQTPNFFGT
                     IEDLAPLAEAAHAAGALLVVCVAEPTALGILKPPGEYGADIVAGEGQALGTGLNYGGP
                     ALGFFATRDKFVRHMPGRLVGQTVDREGRTGYVLTLATREQHIRREKATSNICTSESL
                     IALMATIHLVSLGPRGLREVALTNLRKAAYAKERLAKVRGCGLRFSGPTFNEFVLKTK
                     KKPGAVLAHLFKRQILGGLDLGRFYPELSDCLLVCVTEQNSREEIEALAKALGGVR"
     gene            3325..>3761
                     /locus_tag="AC16G0_08935"
     CDS             3325..>3761
                     /locus_tag="AC16G0_08935"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743290.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HIR5099772.1"
                     /translation="MSGPENTVGTRGLVFNEPLIFDQGSPGRVGYSLPKSDVPEKKPE
                     TLVPKRLLREDIPGLPEVSEVDAVRHFTRMSQWNFGIDLGFYPLGSCTMKYNPRINED
                     MARLPGFARAHPYLPEEAAQGALQLLWELEQFLADVAGMDRVTL"
BASE COUNT          690 a         1177 c         1275 g          619 t
ORIGIN      
        1 cattttctgc cgggcaaata tctttcctgc cccgcccctg ccatcttttt caacccgatg
       61 acgcagacac tctcagcggc tccgagcatg gtctcatcat cgcttcgact tttcccttga
      121 caaagaccca gggccattaa aaaatcacca gcacttttct actcctcgtc ccgatccccc
      181 aagttacagc ctgagagcgt gcatgtccgc gcattctggc cctggtcccg tgatttcggc
      241 acctgcgagg agtggtcccc agccttgtcg gcctgcgctg tctgatggtc ccggcgccac
      301 cgctcgtggc tctcaatccc gagctcggtc cagaccacac actcaggagg aacagcatgt
      361 ccgggcctga aggtcccctg aagcagaccc cgctgaacgc cgtccatcat cagatgggcg
      421 cgaagatggt acccttcggc ggctgggata tgccggtcca gtaccgaggg atcctggacg
      481 agcatcgagc cgtccggagc gctgccgggc tgttcgacgt ctctcacatg ggggaggtgg
      541 agtttcgggg gccggcggcc ctcgaggccg tccagcgact caccaccaat gacgcctccc
      601 gcctcaaggt ggggcaagtg cagtatacgg ccctgaccac cgaggccggg acgttcgtgg
      661 acgacctgac ggtgtacaag ttcgccgacg accattacat ggtcacggtg aatgcctcga
      721 acatcgacaa ggacgtggcc tggatgcgtt cccacaccac aggcagcgtc gaggtccgga
      781 acgccagcga cgagacggcc ctcctggcca tccagggacc cagggccctg gcgatcctgc
      841 agaagcttac tccggtggat ctgggcgcca tcccatacta ctggttcgcg cagggcaagg
      901 tgctgggagt ggacgccgtt ctctcgcgga cgggatacac cggcgaggat gggtttgagg
      961 tatatgtcaa gcccaaggac gtggttgcct tgtggcaggc cttgctggaa gcaggaacga
     1021 cccaggggct gcttccgtgc ggcctggggg cgcgggacac gctccggctc gaagccaaga
     1081 tggccctgta cgggaacgac atcgacgatc ggcacaccgt cctggaggcc gacctgggat
     1141 ggatcctcaa gctgcagaag ggagagttca ttggccgggc ggccctggcc aaacaaaagg
     1201 aggagggcgt tcggcggaag ctcgtcggat tcgagatgat tgggcgaggc atcgggcggt
     1261 cgcactaccc gatcgtgaaa gacgggcggc ccatcgggga ggtcacgagc ggcggcccgg
     1321 ccccctggct caacaagaac atcgggctgg gctacgtggc gcaggagcac gcggcagttg
     1381 ggacggacat cgagatcctg atccggggga ctccggttac cgcccggatc gtgcagacgc
     1441 cgttctacaa gcgaccgcgc tagcggatcg cgagtcggag agcttccaga agcgtgggtg
     1501 cctagctgga ggtggcacaa cccgggatgg ctgcgaagga attgacttcg gttcaagact
     1561 tctgaggggg acccgagcca ggaggagcct gcatgtaccc agccgacctg aaatacacga
     1621 aggagcacga gtggatcaag gtggaaaggg gcctggggcg cgtggggatc acgcatcacg
     1681 cccagaacgc cctcggcgac gtggtcttcg tcgagctgcc gaaggtgggg cgcgccgtga
     1741 agcagatgga gaccttcggc gtggtggagt cggtgaaggc agtctcggac ctgtactcgc
     1801 cggccagcgg cgaggtggtg gaggccaacg gggcgttgga gggaaagccc gagctggtga
     1861 accaggactg ctatggcgcc ggctggatga tcgtggtgaa gctcgccaat ccgaaggagc
     1921 tggatgcgct catggatgcc aaagcctacg aggcgtacct ggcggctgag gggggccact
     1981 gatgcgctac attccgaaca ccgacctgga tcgccgccag atgctggaag ccatcggcgt
     2041 cggctcggtt ctcgacctgt tcgccgatgt tccgagcgag gttcggctca agcgatcgct
     2101 caaggtgcct ccggcccttt ccgaggccga actcgggaag cacctgcgga ccttggcggg
     2161 gaagaacgct gacgcggacc gcttcagcac cttcctgggg ggcggctgct accaccactt
     2221 cagccctgcc atcatcaatc acctggttct gcgcggggaa ttcctcacgg cctacacccc
     2281 gtaccagccc gagatctccc aggggacgct gcaggcccta tatgagtatc agaccctcat
     2341 ttgcctcctc accgggatgg aggtcagcaa tgcctccatg tacgaagggg cgtcgtccac
     2401 ggccgaggcg atcctgatgg cccagcgcgt caccggccgg aatgaggtgg tggtggcccg
     2461 cgcggtgcac cccgagtacc ggcaggtggc gcggacctac acggcgcagg tcgggatcac
     2521 gttcaaagag gttcccttcg ccgaggccgg gcacacggat gccggcatcg tccagcaggc
     2581 cctctccgaa catacagcct gcctggtgat ccagaccccg aatttcttcg ggacgatcga
     2641 agatctggcg cccctggccg aggcagcgca cgccgcaggg gcgctcttgg tggtgtgcgt
     2701 ggccgagccc acggccctgg ggattctcaa gccgcccggg gaatacggcg cggacatcgt
     2761 ggccggcgag ggacaggccc tgggcacggg tctgaactac ggcgggccgg ccctgggctt
     2821 cttcgccacc cgggacaagt tcgtccgcca catgccgggg cggctggtgg gccagacggt
     2881 ggaccgcgag ggacggacgg gatacgtcct cacgctggcc acgcgcgagc agcacatccg
     2941 gcgggagaag gccacctcga acatctgcac cagcgagtcc ttgatcgccc tcatggcgac
     3001 aatccatctc gtcagcctgg gaccccgagg attgcgcgag gtcgccctga cgaacctgcg
     3061 caaggcagcc tacgccaagg agcgcctggc gaaggtccgg ggatgcggtc ttcgattctc
     3121 cggtcccacg ttcaacgagt tcgtcctcaa gaccaagaag aagccgggcg ccgtcctggc
     3181 ccacctgttc aagcggcaga tcctgggcgg cctggacctg gggcgattct atccggaact
     3241 gtcggactgc ctgctggtct gcgtgaccga gcagaacagt cgcgaggaga tcgaggcgct
     3301 ggccaaggcg ctgggagggg tgcgatgagc ggaccggaga acacggtcgg cacccggggg
     3361 ctggtattta acgagccact gatcttcgac caggggtcgc cggggcgagt ggggtactcc
     3421 ctgccgaaga gcgacgtccc cgagaagaag cccgagacgc tggtcccgaa acgtctcctg
     3481 cgggaggaca tcccgggtct gcccgaggtg agcgaggtgg atgccgtccg ccacttcacc
     3541 cggatgtcgc agtggaactt cggcatcgat ctgggctttt atcctctcgg atcgtgcacc
     3601 atgaagtaca acccgcggat caacgaggac atggcgcggc tgccgggctt cgcccgcgcc
     3661 catccctacc tgccggaaga ggcggcgcag ggggcgctcc agctcctgtg ggagctggag
     3721 cagttcctgg ccgacgtggc gggcatggac cgggtcacgc t
//
LOCUS       DBNGZC010000509         2780 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_726347, whole genome shotgun sequence.
ACCESSION   DBNGZC010000509 DBNGZC010000000
VERSION     DBNGZC010000509.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_726347"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..1176
                     /gene="larE"
                     /locus_tag="AC16G0_08940"
     CDS             346..1176
                     /gene="larE"
                     /locus_tag="AC16G0_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HIR5099773.1"
                     /translation="MTPTGLASLPPALASRYEALQDLLRDMGRVLIAYSGGVDSAFLL
                     AVAAECLGTDVLAVTGLSPSIAAAEVEEASRLAQQIGAEHRFIQTSEMERPGYVANAP
                     DRCYFCKTDLFERLRTLAQAEGYAWVADGSNLDDQGDLRPGREAARQQGVRSPLVEAG
                     LTKAAIRELSRAMDLPTWDKPAMACLASRIPHGTPVTIERLGQVEAAEAALRALGFQG
                     VRVRHHESIARIELPPEERGRLLDPVLASEVAQRVKAAGYQYVVLDLEGYRPGGRPKT
                     "
     gene            complement(1700..>2780)
                     /locus_tag="AC16G0_08945"
     CDS             complement(1700..>2780)
                     /locus_tag="AC16G0_08945"
                     /inference="COORDINATES: protein motif:HMM:NF037016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cadherin-like domain-containing protein"
                     /protein_id="HIR5099774.1"
                     /translation="IDPVTGQLVVRAQLAARSKVTVLSSGRGENQLQMTTGTVTGGVG
                     GGATTPVPVALNDTAATLEDTAVTVNVLANDSNALGGTVSLVSPALLGSAVVNANGSI
                     TYTPNLNANGTDTLAYTVTVGTQVSNSATVTLTITPVNDPPTAISDTAGALRGVSNSV
                     NVFANDVDPDGKADLANAVIVTGNASLGITPGTVFPGGIVTFTPPATTAAGPYVFTYN
                     AVDLAGAVSATPATVTVNVSTTEAIVVAKDIYTQTKGRWTVQGTISPAAGQTMTIRYV
                     DGIFKVNGVCPATSNAAGTVIGTAGVDALGNWLFDTILPSTAGVLNPSNTLGNSTGFW
                     CTPPKSVRITSSLTGGSVTSAISLK"
BASE COUNT          433 a          912 c          950 g          485 t
ORIGIN      
        1 tttcaatact aggcgtcccg gagggacgct gcgaaagtag cccggggcta gcgaagcgca
       61 gccccgggtt ggcagtcgac aggaaaccga gccccgggag gggcgacaga agcgactgta
      121 attgagggaa ttctgccgcc cctgaccggg gcttgatcca ttgaactttc acaatcccgg
      181 gcggcgcttc gctggcccgg ggctactttc tgatcgccgc tccgcggctt gcacggggct
      241 gtttgaggat ccggaccagg agatcaccgg ccggttcttc gtcgtgccgg agatggcgtc
      301 gctgcaaagg atgatccacg gggcgggaga tgggaggtcc cggccatgac gcccaccggc
      361 ctggcgtccc ttccgccagc cctggcttcc cgctacgagg ccttgcagga tctcttgcgg
      421 gacatggggc gcgtgctgat cgcctactcc gggggggtgg acagcgcgtt cctcctggcg
      481 gtggcggccg agtgtctggg aacggatgtc ctggccgtca ccggattgtc tcccagcatc
      541 gccgccgccg aggtcgagga ggcgtcacgg ctggcccagc agatcggcgc cgagcaccgc
      601 ttcatccaga cgtccgagat ggagcggccg ggctatgtgg ccaatgcgcc ggaccgatgc
      661 tacttctgca agacggacct cttcgagagg ctccggaccc tggcccaggc tgagggatat
      721 gcctgggtgg ccgatgggtc gaatctggac gaccagggag acctgcgccc cggccgggag
      781 gcggcccgcc agcagggggt gagaagcccc ctggtcgagg ccgggctgac caaggcggcg
      841 atacgggagc tgtcccgcgc catggatctc ccgacctggg acaagcccgc catggcctgt
      901 ctcgcctcgc ggatccccca cgggacgcct gtcaccatcg agcgactcgg acaggtcgag
      961 gccgccgaag ccgcgctccg cgccctgggc ttccagggag tgcgcgtgcg acaccacgag
     1021 agcatcgcgc ggattgagct accgcccgag gaacggggac gcctcttgga cccggtcctg
     1081 gcctcggagg tcgcccagcg ggtgaaggcc gcgggctacc agtacgtcgt cctggatctg
     1141 gagggctacc gccccggcgg ccggccaaag acctgatccg tcccccgcca cgctccggat
     1201 ctctcgcctt tcccacgctg tcttctccgg tgttgtcacc tcatactacc ccagcgtacc
     1261 atcgtttcct cgctcttctc cgccccttca cggtgctggt gctggtctcg gcctcttcct
     1321 gcgtgttctc taccgcgggg ccgggctcag cccgaataat gcacttcaac cgcagagcac
     1381 gccaagcacg cagagaagac gcctttggat ctcagggact cctgaggcag ggcccactgg
     1441 gatgccggaa catttcggct ccttactcag gggctgaagc ctccaactac gtctgccttt
     1501 gcgaactttg cgatgagata atccggttca ggggcgagtt ggggttctgc aatgaaaaaa
     1561 cccggcgggc ggagtgccta gttactggct tctccgcccg ccgggcaacc tggggacggc
     1621 gccaccgcag ccccagggag tcccctggcc gggcgctccg acgaggatta tctcgtgggg
     1681 cgcccgaggc caggtcagcc tacttcaacg agatggccga ggtaacgctg ccgccggtga
     1741 gcgatgaggt gatccggacc gacttcggcg gcgtgcacca gaagccggtg gaattgccga
     1801 gcgtgttgct cggattcaga acgccggcgg tgctgggcag gatcgtgtcg aacagccagt
     1861 tgccgagggc gtccacaccg gccgtgccga tcacagtgcc ggccgcgttg ctggtggcgg
     1921 ggcaaacccc gttgaccttg aagatgccgt caacgtacct gattgtcatc gtctgacccg
     1981 cggccgggct gatggtgccc tgcacggtcc agcggccctt ggtctgcgtg tagatgtcct
     2041 tggcgacaac gatggcctcc gtcgtggaca cgttcacggt caccgtcgcc ggggtcgcgg
     2101 acaccgcgcc cgccagatcc acggcattgt acgtgaacac gtacggcccg gccgcggtcg
     2161 tggcgggcgg ggtgaaggtc acaatgccgc cagggaagac cgtgcctggc gtgatgccca
     2221 gactggcgtt ccccgtcacg atcacggcat tggcaagatc tgccttgcca tccggatcaa
     2281 cgtcgttggc gaacacgttg acgctgttcg agaccccccg gagcgcgccg gcagtgtcgc
     2341 tgatcgcggt cggcgggtcg ttcaccgggg tgatcgtgag cgtcacggtg gccgagttcg
     2401 aaacctgcgt cccgaccgtc accgtgtagg cgagggtgtc ggtgccgttg gcgttcaggt
     2461 tcggcgtgta ggtgatgctg ccattggcat tgaccaccgc actgcccagc agggctgggc
     2521 tcaccaagct cacggtcccg ccgagggcgt tgctgtcgtt ggccaggacg ttgacggtca
     2581 cagccgtgtc ctccagggtt gccgccgtat cattcagcgc caccgggaca ggggtggttg
     2641 cgccgccccc gacaccgccg gtgacagtgc ccgtggtcat ctggagctgg ttctcgccgc
     2701 gcccggaaga gagcaccgtc accttggacc gcgccgcgag ttgtgcccgg accacgagct
     2761 ggccggtcac gggatcgatg
//
LOCUS       DBNGZC010000510         1786 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1705356, whole genome shotgun sequence.
ACCESSION   DBNGZC010000510 DBNGZC010000000
VERSION     DBNGZC010000510.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1705356"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..110)
                     /locus_tag="AC16G0_08950"
     CDS             complement(<1..110)
                     /locus_tag="AC16G0_08950"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745380.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR5099775.1"
                     /translation="MARKRVLSGMRPTGRLHLGHLFGALDNWKTLQEQYD"
     gene            complement(196..831)
                     /locus_tag="AC16G0_08955"
     CDS             complement(196..831)
                     /locus_tag="AC16G0_08955"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR5099776.1"
                     /translation="MFELPSIQTLVITIPVFLLALTIHEFAHGWVANWLGDPTARLQG
                     RLTLNPIAHLDPIGTLAIVFIGFGWARPVPVDARYLKRPQRDMMLIAAAGPGSNLLLA
                     VVSAFCVRMIPWSVASPDWMWLVKPVLLMLYTSVGANVILAVFNLLPIPPLDGSRVLT
                     GLLPLRQAINFSRLEPYGGIIIFLLFITGIMNPMFGLAQHTITRALLRMWG"
     gene            complement(858..>1786)
                     /locus_tag="AC16G0_08960"
     CDS             complement(858..>1786)
                     /locus_tag="AC16G0_08960"
                     /inference="COORDINATES: protein motif:HMM:NF024041.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA nucleotidyltransferase/poly(A)
                     polymerase-related protein"
                     /protein_id="HIR5099777.1"
                     /translation="ELLDFFGGQQDLRDKTLRIIHNLSFVEDPTRILRAVRFEVRFGF
                     HLSRHAEQLITNAVQMGLLAKLSGARLFTELQLILQEARPLAILQRLQELKVLTAIHS
                     HLSVDATTERRFRRVGEVLTWYGLLYQGEQPTPWIVYLLTLLGERVGTEARAILRRLG
                     PSPRAAGTLVQDLARLRGLARQFQQARELAPSRVFRWLAEAPLEVVLALMARMERPEL
                     RKAVGDFLATMRRVRPFLRGSDLRTLGIRPGPIYREILNSLQYARLDGHVQSRDDELR
                     FVRQKFSHALATSDTETAAARLTNRRGSGRVH"
BASE COUNT          341 a          577 c          576 g          292 t
ORIGIN      
        1 cagtcgtatt gctcttgcag cgtcttccag ttgtcgaggg ctccgaagag atgccccagg
       61 tgcagccgcc cggtcgggcg cattccgctc aaaacccgct ttctcgccat gccgtctccg
      121 tcggaacgtg tgatcgcatc tcggggctcg cggctcgcat gtcgtggccc gcggctcgcg
      181 gctctctgcc cgcccctacc cccacatacg caacagggcg cgtgtgatcg tgtgctgcgc
      241 aagcccgaac atcggattca tgatgcccgt gatgaacagc aggaatatga tgatgccccc
      301 gtagggctcc aggcggctga agttgatggc ctgtcgcaga ggcaaaaggc cggtgaggac
      361 ccggctgccg tcgagaggcg ggatgggaag aagattgaac actgccagga tgacgttggc
      421 gcccaccgac gtgtacagca tcaacaggac gggcttgacg agccacatcc agtccggact
      481 cgccaccgac cacgggatca tgcgcacgca gaaggcggaa accacggcca gcagcagatt
      541 gcttccgggt ccggctgcgg caatcagcat catatcccgc tgcggtcgct tgagataacg
      601 cgcatccacc gggaccggcc tggcccagcc gaagccgatg aacacgatgg ccagcgtccc
      661 gatggggtcg agatgggcga tgggattcag cgtgagacga ccctgaagac gcgccgtggg
      721 atcacccagc caattggcca cccagccgtg cgcaaactcg tgaatcgtga gagccagaag
      781 gaagacagga atggtgatga cgagagtttg aatcgagggc agttcgaaca tagggaccct
      841 tcttgacttg ccggaaatta atgaacccta cccgatcctc tccggtttgt caatcgggcg
      901 gcggcggttt ccgtgtcgga tgtggcgagc gcgtgggaga acttctggcg cacaaatcgc
      961 aattcgtcgt cccggctctg cacatggccg tcgagcctcg catactggag cgagttcagg
     1021 atctcgcggt agatcggccc cggccggatg cccagggtcc ggagatcact cccccgcaag
     1081 aacggccgga cccgacgcat cgtggccagg aagtcgccga ccgccttccg aagctccggc
     1141 cgctccatcc gggccatgag agccaggacg acctccagcg gtgcttccgc cagccagcgg
     1201 aatacccggc tgggagccag ctcccgcgcc tgctggaatt gtcgcgccag cccccggagc
     1261 ctcgccaggt cctggacgag tgtcccggcg gcccgcggcg acggtcccaa tcggcgcagg
     1321 atcgcccggg cctcggtacc gacccgctcc cccagaagcg tcaggaggta cacgatccag
     1381 ggcgtgggct gctccccctg atacaagagg ccgtaccagg tgaggacctc ccccacccga
     1441 cggaagcgtc gctccgtggt cgcgtccacg ctgaggtgcg agtggatggc ggtcaggacc
     1501 ttcaattcct gcaatcgctg gaggatcgcc agggggcgcg cctcctggag gatcaattgc
     1561 agttcggtga acagccgggc ccccgacagc ttcgctagca gccccatctg gacagcattg
     1621 gtgatgagtt gctcggcgtg ccggctcaga tgaaagccga accgcacctc gaagcggacc
     1681 gcgcgcagga tgcgcgtcgg gtcctcgacg aaactcaggt tgtggatgat gcggagcgtc
     1741 ttgtcgcgca ggtcctgctg cccgccgaag aaatccagaa gttcgc
//
LOCUS       DBNGZC010000511         2137 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2587718, whole genome shotgun sequence.
ACCESSION   DBNGZC010000511 DBNGZC010000000
VERSION     DBNGZC010000511.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2587718"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1191)
                     /locus_tag="AC16G0_08965"
     CDS             complement(<1..1191)
                     /locus_tag="AC16G0_08965"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5099778.1"
                     /translation="MEPDAMEKQGGPDRTAAAAAKEAPDRLVGQLIRARYRVGAKLAV
                     GQTGTVYAAQQLNTGNKIALKILHGPVAKNEEAVRRFRQLMTALRALSRNQQSIVRVY
                     DFDQAEDGRLFVAMEFLAGTPLSDVIRQAGPLNIEKALRLARQMAEGLGVAHEFGIVH
                     TDIRPQHFMIVGEEESIKVIGFERARLKDGGGMDLPVESEGLARTPEYLAPEQIRGEE
                     ITRQTDIYAFGVVLYEMLSGVTPFRAETPEALRAMHLRDAPPPLKELRPEIPGVVEAK
                     VLQALEKDPKLRETYVNDVVNEYLYESALFELGEKKDQAKAGRVSRVQPASESDVEEE
                     QDDVADGSWLRWKLVLIGVALLLIVAASLWMFSSRRVPEDAAVPAPQPRPSQVRPPAP
                     VAPEK"
     gene            complement(1348..>2137)
                     /locus_tag="AC16G0_08970"
     CDS             complement(1348..>2137)
                     /locus_tag="AC16G0_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR5099779.1"
                     /translation="YAVFQRPGVQLGIVQADVLAFVARVETNAVLQRIAKKTKMVLPL
                     YNEEVHLVGRRDIADFDDLADKRVAIGREGSGTYLTTRLLFKLSEVVPKEMVLIDTDE
                     ALAELKAGRIDAMFYVAGFPVKLFTEGVSEADGLSVIPITNKSILEFYPRTQIPANTY
                     AWQPNPVDTVAVKAVLVSYDYKKADCDNVGRAAEIISSNQDWLVKNGHPKWKVVDLNY
                     PLKGWEQYECVRRYLKKPARATPGRSDKTPVNPVLDAIKDVLGE"
BASE COUNT          345 a          742 c          625 g          425 t
ORIGIN      
        1 tttctctggg gccaccggcg cgggtgggcg gacctggctt ggacgcggct gcggcgcggg
       61 cacggccgca tcctctggaa cccgtcgaga cgagaacatc cacagagagg ccgccacgat
      121 caagagcagt gcgacgccta tcaggaccag cttccagcgg agccacgagc catcggcgac
      181 gtcgtcctgc tcctcctcca catccgattc gctggccggc tggacccggc tgacccgtcc
      241 cgccttggct tgatctttct tttcccccag ctcaaagagc gccgactcgt agaggtactc
      301 gtttaccacg tcattcacat acgtctcgcg cagtttcggg tccttctcca gcgcctgcaa
      361 gaccttcgcc tcgaccacgc ccgggatctc gggcctcaac tccttcagcg gaggcggcgc
      421 gtcccgcagg tgcatggccc gcaaggcctc aggcgtctct gccctgaatg gggtgacccc
      481 gctcagcatt tcgtagagga ccacgccgaa ggcatagatg tcggtctgcc tggtgatctc
      541 ctccccgcgg atctgttccg gcgcaaggta ttccggagtc ctcgcgagtc cctcggactc
      601 gacggggagg tccatgcccc cgccatcctt cagtcgtgcc cgctcaaagc cgatcacctt
      661 gatcgactcc tcctccccga cgatcatgaa gtgctgcggc cggatatccg tgtgcacaat
      721 cccgaactcg tgcgcgactc caagtccctc ggccatctgt cgagccaggc gcaacgcctt
      781 ctcgatgttc aagggcccag cctgccgaat gacgtcgctc agaggggttc cggccaggaa
      841 ctccatggcg acgaaaagcc gaccgtcttc cgcctgatcg aaatcgtaca ccctcacgat
      901 gctctgttga ttccgggaca gagccctgag cgccgtcatc aactgtcgga accggcgcac
      961 ggcctcctca ttctttgcca cggggccatg caggatcttc agggcgattt tgttccccgt
     1021 gttcaactgc tgggcggcat ataccgtccc cgtctgtcca acggccagct tggctcccac
     1081 ccggtaacgt gcccggatca gttggcccac cagccgatcg ggggcctcct tggccgcggc
     1141 ggcggctgtc cggtccggcc cgccctgctt ctccatcgcg tctggctcca tggcatcccc
     1201 cgcccctgcg tcacggcgac aggagcagtc ggacgtgggg atgccgcacg acctgcggcc
     1261 ttccgtccca cccgtcacgc ccggtcccgt gaaggaggag gagcccttcc ccccgcccgc
     1321 tgggtggtct cccagggcaa ccgcgggtca ctcccccagg acgtccttga tcgcgtccag
     1381 cacggggttg acgggcgtct tgtccgagcg gcccggggtg gcacgcgcgg gtttcttgag
     1441 gtaccgtcga acgcactcgt actgctccca gcctttcagc ggatagttca aatcgacgac
     1501 cttccacttc gggtgtccgt tcttcacgag ccagtcctgg ttgctggata tgatttcggc
     1561 ggcccggccc acattgtcac agtcggcctt cttgtagtcg taggacacga ggacggcctt
     1621 cacggcgacg gtgtccacgg gattcggctg ccaggcatag gtgttcgccg ggatctgcgt
     1681 tcggggataa aattccagga tgctcttgtt ggtgatcggg atcacactca acccgtcggc
     1741 ctcgctcacg ccttccgtga acagcttgac gggaaatccc gcgacataga acatcgcgtc
     1801 gatgcggccg gccttcaatt ccgccagggc ctcgtccgta tcgatgagga ccatctcctt
     1861 cgggacgacc tccgagagct tgaagaggag cctggtcgtc aggtacgtgc cgctgccctc
     1921 tctgccgatg gcgacccgtt tgtcagccag gtcatcaaaa tccgcgatgt cgcgtcgccc
     1981 caccaggtgg acctcttcat tgtacagggg aagaaccatc ttcgttttct tggcgattcg
     2041 ttgcagcacg gcgttggtct ctacgcgggc aacgaaggcc aagacgtccg cctggacgat
     2101 tcccagctgg acgccgggcc gttggaacac cgcatac
//
LOCUS       DBNGZC010000512         1493 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_238337, whole genome shotgun sequence.
ACCESSION   DBNGZC010000512 DBNGZC010000000
VERSION     DBNGZC010000512.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_238337"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1493)
                     /locus_tag="AC16G0_08975"
     CDS             complement(<1..>1493)
                     /locus_tag="AC16G0_08975"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR5099780.1"
                     /translation="VAIWLLFPVLKIFHEMGHALTTKAFGGEVHSMGIMLLVFSPLPY
                     VDASSSSSFPSKWQRIVVGAAGMLVELFLASLALFVWLAAEPGIVRAAMYDIMLIAGL
                     STVVFNINPLLRYDGYYILSDFLEIPNLYTRSSAYLSYLCQRYLFGRHEVEEPLASWS
                     ERAWFVFYAVSAFAYRLLVILLVSLVLLGKSFYLGVVGVFALLVGWLGLPLWRGLRFL
                     TTGSSLRGNRFRAITVSLALVGLVVGGVGFVRIPSRTTTEGVVWVPDEALVRAGVDGF
                     VDRIVARPGSDVHSGDVLIECRDPELETRARVVAGQVKELRARYDEQRPTDLVKAAQI
                     QDQIRYAEEDLARTRKQLSELTIRSRTEGTFIVPEVQDLPRRYVKRGDLLGYAVHLDR
                     ITVRAVVSQADIDLVQHETKSVTVRLSERLGQQQPAVIRREVPGAMDRLPSAALGYSG
                     GGNVAVDPRDAKGVTAMDKVFQIDLELPSNLPLLNLGSRVYVRFDHG"
BASE COUNT          312 a          500 c          468 g          213 t
ORIGIN      
        1 cccgtgatcg aagcggacgt acacccggct ccccaggttc aggaggggga ggttcgacgg
       61 cagctccaga tcgatctgga agaccttatc catggccgtc acaccctttg catcccgtgg
      121 atcgacggcc acattcccac ccccactata ccccagggcc gcgctgggca gccggtccat
      181 ggccccggga acctcgcggc gaatgacggc tggctgctgc tggccgagcc gctccgagag
      241 gcgcaccgtg accgacttgg tctcatgctg caccaggtcg atatcggcct gggacacgac
      301 ggcgcgcacc gtgatccggt cgagatggac ggcatacccc aggaggtctc cgcgcttcac
      361 gtagcgccga ggcagatcct ggacctccgg cacgatgaag gttccctccg tgcggctccg
      421 gatggtcaac tccgagagtt gcttgcgcgt gcgggccaga tcctcctcag cgtatcggat
      481 ctgatcctgg atctgggccg ccttcaccag gtccgtcggg cgctgttcgt catagcgggc
      541 ccggagctcc ttgacctgtc ctgccaccac ccgtgcccgc gtctccagct ccgggtcccg
      601 acactcgata agcacatccc cggaatgcac gtcggacccc ggccgggcca cgatccgatc
      661 cacgaaccca tccactccgg cccgcaccaa ggcctcatcg ggcacccaca ccaccccttc
      721 ggtggttgtc cgggatggga tgcgcacgaa accgacacca cccaccacca ggccaaccag
      781 ggccagggaa accgtgattg cccggaaccg attgcctcgc aggctcgaac cagtggtgag
      841 gaagcgcagg ccgcgccaga gaggcaaacc cagccagccg accagcaggg cgaacacccc
      901 gaccaccccc aggtagaagg acttaccgag cagaaccagg gacaccagga ggatgacgag
      961 gagccgatag gcgaaggcgc tgacggcata gaatacgaac cacgcgcgct ccgaccagga
     1021 ggccaggggc tcctccacct cgtgacggcc gaataggtag cgctggcata gatagctcag
     1081 gtaggcggaa gaccgcgtgt acaggttggg gatctccaga aaatccgaga ggatgtagta
     1141 cccgtcgtac cgtagcagcg ggttgatgtt gaacaccacc gtggaaaggc cggcgatgag
     1201 catgatgtcg tacatggcgg cccggacgat ccctggctct gccgccagcc agacgaagag
     1261 ggccagggag gccaggaaca gttccaccag catgcccgcc gcgccgacga cgatccgctg
     1321 ccacttgctg ggaaaggatg aggaggaaga ggcatccacg tagggcaagg gcgagaacac
     1381 caggagcatg atgcccatgc tgtgcacctc gccgccgaag gccttggtgg tcagagcgtg
     1441 ccccatctcg tggaagatct tgaggaccgg gaagagcagc catatggcca caa
//
LOCUS       DBNGZC010000513         4294 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_339330, whole genome shotgun sequence.
ACCESSION   DBNGZC010000513 DBNGZC010000000
VERSION     DBNGZC010000513.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_339330"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..709)
                     /locus_tag="AC16G0_08980"
     CDS             complement(26..709)
                     /locus_tag="AC16G0_08980"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR5099781.1"
                     /translation="MAVITISREVGSGGDQIAHQVCELLEYRYFDKLLMAQVAREQGV
                     SETEVVDFSEDKYRLRGFLDALLRRSARVALATTLATTKSGAVTRVVHSVDEEMAAAF
                     VSATIRALWTRGRIVVVGRGGQAILRDVAGVLHVRIIARLEDRVWQVMQAEGLTRDVA
                     LRTIDERDRATTQYLHRFHGIDWDDPLLYHLTLNASVMDRETAAGVIATLARQMEAHP
                     TEPPTPRAG"
     gene            complement(963..1310)
                     /locus_tag="AC16G0_08985"
     CDS             complement(963..1310)
                     /locus_tag="AC16G0_08985"
                     /inference="COORDINATES: protein motif:HMM:NF020317.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIR5099782.1"
                     /translation="MSLYMYQFKYTSAAWAAFAKKPEDRRAVVDELLRKLGGRLVSAY
                     YTFGEWDGLVIYEAPNEISAMAAIVTIIAPGHIEATKTTVFFTIDDAIKALDMAGALK
                     FRGIEPLPSSWPA"
     gene            2255..2428
                     /locus_tag="AC16G0_08990"
     CDS             2255..2428
                     /locus_tag="AC16G0_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099783.1"
                     /translation="MATELQSLRQAREFLTRKGDPDKAGEEVTMKREVRARTIWSSIL
                     GVRAAALFPRPMT"
     gene            complement(2425..3396)
                     /locus_tag="AC16G0_08995"
     CDS             complement(2425..3396)
                     /locus_tag="AC16G0_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR5099784.1"
                     /translation="MAFGDTFSALFAGRVRAHLDIQCHFTLPDERGNIPRLSEMDVLV
                     TLAFTREMGAVARRLKLVQVPGAGVDRIDRSALPPGAWLANAYGHETGIAEYILGAML
                     AWTRDLTRLDARLRQGTWESVWAIPPAPPAPWPELAGKTLGILGYGRIGQCVARRARA
                     FDMLVWAIRRDAMRSETDGLAFLGGPDALDEVLKHADYLVLTLPLTAATRGLLGEREL
                     QLMKSTALLINVARAEIIDEVALYRALAKKTIAGAVLDVWYRYPTGAGLTLPSRHAFH
                     ELSNVLMTPHVSGWTEGTLDARARLIAENIHRVARGDPPLNLIRLSD"
     gene            complement(3559..>4294)
                     /locus_tag="AC16G0_09000"
     CDS             complement(3559..>4294)
                     /locus_tag="AC16G0_09000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013921328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5099785.1"
                     /translation="LASNTILITGGASGIGFALAQLFLRAGSEVIICGRRPEQLQAAQ
                     DALPQIKIRVCDVAKDSDRLALCRGVVGEFAGLNVLVNNAGIQRRPQLTQPEEWEQTR
                     QEIAINFEAPVHLSTLLVPHLLKQQRPAIVNVTSGLGFVPMARAPIYSATKAALHSFT
                     LSLRQQLSHTPIQVVEIIPPAVNTDLGGAGLHTFGVPLDEFADAVIAGMQNGDLEIAY
                     GFSQQASRASRAQLDELFTRMNQTAS"
BASE COUNT          826 a         1401 c         1291 g          776 t
ORIGIN      
        1 tcacttcctc ctcgtatctg cccacctaac cagcccgggg tgtaggcggc tcagtcggat
       61 gcgcctccat ctgccgcgcc agcgtggcga tcactcccgc cgccgtctcc cggtccatga
      121 cggaggcgtt gagcgtcagg tggtagagca acgggtcgtc ccagtcgatg ccgtggaatc
      181 ggtgaaggta ctgggtcgtc gcccggtcgc gctcatcgat cgtgcgaagg gctacgtccc
      241 gagtgagtcc ctcggcctgc atcacctgcc agacccggtc ctcaagccgg gcgatgattc
      301 gcacgtgaag caccccggcc acatcccgga gaatcgcctg gccgccccgg ccaaccacaa
      361 caatccgccc acgcgtccag agggcgcgga tcgtcgccga cacgaatgcg gccgccatct
      421 cttcatccac agaatggacg actctggtca ctgcaccgct cttcgtggtg gccagcgtgg
      481 tggccagggc gacccgcgcc gagcgcctca acaaggcgtc gaggaacccg cgcagccgat
      541 acttatcctc ggagaagtcc accacctccg tctcggagac cccctgctcc ctggccacct
      601 gggccatcag caacttgtcg aagtagcgat actccagaag ttcgcacact tgatgggcga
      661 tctggtcgcc cccgctgccg acttcccggg aaattgtgat caccgccatg gctcacacct
      721 cccgcccgga ctgtttgccc gcctggtcag gatccatgcc ggcgtcggaa tctccaagtt
      781 acccgcaagg cccccattcc cacgcagcat cgttcagtct cttgcttcga ggcttccggt
      841 ccgtgatctc ccctccgtcg cctgcagcaa gtctcttcct ggctcgggcc gggagaaccg
      901 ctgccatcac gtccaacccc ccggagccat cccactctgg ggtgggaaaa ctcggggcga
      961 ctttacgccg gccatgaaga tggcagtggc tctatccccc gaaatttcag cgctcctgcc
     1021 atatccagcg cctttatcgc atcgtcaatg gtgaagaaca cagtcgtctt ggtcgcctcg
     1081 atgtgcccgg gagcgatgat tgtgacaata gccgccatcg ccgagatctc attgggggcc
     1141 tcgtagatga ccaatccatc ccactcgccg aatgtgtagt aagcggaaac cagtctcccg
     1201 cccagcttcc tcagcaactc gtccacgacc gcacgccggt cctccggctt cttggcgaat
     1261 gccgcccatg cagccgaggt atatttgaac tggtacatgt aaagtgacat ggcttttcct
     1321 ccccctcgtc acgggttctt gtaggaatga aaggcgatct cgcagctcca gcgtttctct
     1381 ggggccagac tattcctcct ttcagtacct cgtgatcccc gtccgtgggg agcagacttg
     1441 tcttgatggc gatcacgctg gacctctttc agacaagacc ctcccggctc ccgggatacc
     1501 gcctctccac gggatctgtg catcgaagga aggggctgcc cgtcgcgcac ccttccgaac
     1561 agggtagacg ccaggcggcg ccggtcccgc atatgctttg gaggcaagtt ccagtctgag
     1621 aagctcctat tcggagcgaa gaagaccggg ttgagggctg ggatagccga tgggcgaact
     1681 tgattcaatt ggaatggagg tcgaaggcgt gaagacctgc tccccagcgc gaggaggacc
     1741 tccggaattc gccgtgacca acggaacctc agaagatcgg cagaggccag cggactgatc
     1801 gccggcctcc tggggagcct ggaccgcata aagcggtcca caacccccca ataagtgaac
     1861 gcgaaagagg gcctcctctc cctcaattcc gacgggagat ttcgccgcag gaggttttcc
     1921 agcatctgcg tggcctccca gggcctgatg acgacccgac accttgtcag gaatcctcag
     1981 gctcgtcgac ttcgccacgc ttgcgtggat tctagctata atatcgtgtc ggagagggaa
     2041 tcccacaagg gtccgcccat gggctgccga gttcctcgcg atcacagtca caaccgccga
     2101 cgtgacaagc tttcccgggg gacttccaaa ttgactaatt gcttttcccc gtgatcaggg
     2161 gagtaggcta tcctgcccca gacaatggcg tcggccgagc gacgtcagcg gggctagggt
     2221 tgccgggcga ggtatccgca ttcaaacagg tggaatggct accgaacttc aatccctccg
     2281 acaggcaaga gagtttctga cgcggaaggg ggatccggac aaggcaggag aagaggtaac
     2341 catgaagcgg gaggtgagag cccgcaccat ctggtcctcc atcctcggcg tgcgggccgc
     2401 cgcactcttt ccccgaccca tgacctaatc cgagagccga atcaggttaa gcggggggtc
     2461 gccccgggca acgcggtgga tattctccgc gatgagcctc gcgcgtgcgt ccagcgtccc
     2521 ctcggtccac ccggacacgt gtggggtcat gagcacgtta gacaactcgt gaaaagcgtg
     2581 gcgtgaggga agcgtgaggc cagcgccggt gggataccga taccagacat ccagcaccgc
     2641 tccggcaatc gtctttttag ccagcgcccg gtaaagtgca acctcgtcga tgatctcggc
     2701 gcgggccacg ttgatcagga gtgccgtcga cttcatcaac tggagttccc gctcccccag
     2761 caggccgcgc gtggccgccg tgagcggcag cgtcagcacc aggtaatcgg cgtgcttcag
     2821 aacttcgtcc aaagcatcgg gtcccccgag aaacgcgagc ccgtcggtct cggagcgcat
     2881 cgcgtcgcgc cggatagccc agaccagcat gtcgaaggcc cgggcccgcc tcgccacgca
     2941 ctgcccgatg cgcccatagc cgaggatgcc cagcgtcttc ccggccagct ctggccaggg
     3001 cgccggggga gccggaggga tcgcccagac gctctcccaa gtgccctgcc ggaggcgagc
     3061 atcgagccga gtgaggtcgc gggtccacgc cagcatggcc ccgagaatgt attccgcgat
     3121 cccggtctca tgaccgtaag cattcgccag ccacgcaccg ggcggtagcg ccgaccggtc
     3181 gatccggtcc acgcccgcgc cgggaacttg caccagcttg agccgtctgg cgacagcacc
     3241 catctcgcgg gtgaacgcca aggtgaccag cacgtccatc tcggacagtc gagggatatt
     3301 cccccgctca tccggaaggg tgaagtggca ctggatgtcg agatgggccc gcacgcgacc
     3361 cgcgaagagg gcagagaacg tgtcgccaaa ggccacccga agtatcggag gtgtcgcatc
     3421 tgaattcaat ggagtgactc cctcagagcc ggaccggtcc ggccccttga gatcctgttg
     3481 actctggcta tccaagacaa ggcgctagcc acgaggtcgg atgcagacgc ctccgggccg
     3541 tgcgaacttt ccgtgcagtc aggacgctgt ttgattcatc cgggtgaaga gttcatccag
     3601 ctgtgcacgc gacgcacggc ttgcctgttg cgaaaagcca taggcgattt ccagatcccc
     3661 attttgcatc ccggcaatga cggcgtctgc aaactcgtcg aggggtacac cgaacgtgtg
     3721 cagaccagcc cctcccaaat ctgtgttcac ggccgggggg atgatctcaa cgacttgaat
     3781 cggcgtgtgg gagagttgct gcctgaggga cagcgtgaag gagtgcaagg ccgctttggt
     3841 ggcgctgtaa atgggagcgc gcgccatcgg cacgaagccc aggccggacg tgacgttcac
     3901 aatcgcaggc cgctgttgct tcaggaggtg gggaaccagc aaggttgaga gatggacggg
     3961 tgcctcgaag ttgatcgcga tctcctggcg cgtctgctcc cactcttcgg gctgcgtgag
     4021 ctgcggacgc cgctgaatgc ccgcattgtt gacgagaacg ttcaatccgg caaattcacc
     4081 aaccaccccc ctgcacaacg ccaggcggtc ggagtccttg gcaacatcgc agacgcgaat
     4141 cttgatttgt ggaagcgcgt cctgcgccgc ctgcagctgc tccgggcgac gtccgcaaat
     4201 gatcacttca ctgcccgccc ggagaaagag ctgggcgagc gcgaacccga ttcccgacgc
     4261 ccctccggtg atcaggatgg tattggaggc gagg
//
LOCUS       DBNGZC010000514         3839 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_339401, whole genome shotgun sequence.
ACCESSION   DBNGZC010000514 DBNGZC010000000
VERSION     DBNGZC010000514.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_339401"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="AC16G0_09005"
     CDS             complement(<1..766)
                     /locus_tag="AC16G0_09005"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HIR5099786.1"
                     /translation="MDISLPSISWGAMLPMLLITAAGLLVLILDPLTPPERRDRLAVL
                     SLAGILAAAVAQLLRHGQTEIVFGGMLAADEFAFFFNLLFLLVAGLTLLISIPYIHSV
                     DMDHGEYYALLLFSTLGMMIMASSLDLMTIFLGLETLSISLYILAGFLREQLKSNESA
                     LKYLLLGAFASGFVLYGIALVYGATGSINLKRVAEALAGGKVHNLTLLVVGMGLLIIG
                     FGFKVASVPFHMWTPDVYEGAPTAVTAFMSVGPKAAG"
     gene            complement(756..2378)
                     /locus_tag="AC16G0_09010"
     CDS             complement(756..2378)
                     /locus_tag="AC16G0_09010"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HIR5099787.1"
                     /translation="MSDFPILSIVIFLPCAGAVLLLGLDRRHEGAIKGLAFGVSLLTF
                     LVSLLLYSGFQLNVPGMQFVEQRPWIAGFNVQYFVGVDGISLLLVLLTTFLTAIAILS
                     SFTAITTSVKEYMICMLLLETGMLGVFVALDLVLFYLFWEGMLIPMYFLIGVWGGPNR
                     IYATVKFFLYTMAGGVLMLVGIISLYYLHGGQPGAVFTFDLLKISAELTLPFATQAWL
                     FLAFAVAFAIKVPMFPFHTWLPDAHVEAPTAGSVILAGVLLKMGTYGFLRFALPLFPE
                     ATRFFTPLFSWLAIIGILYGALVSMVQPDLKKLVAYSSVSHLGFVMLGIFALTSQGLE
                     GGILQMINHGLSTGALFLLVGMIYERRHTRLIEEFGGLARVIPAFTAFFMIVTLSSIG
                     LPGLNGFVGEFLILVGTFQVNILYAVLGTAGIILAAVYMLWMFQRVMFGTLTKEENRG
                     LIDLTPREIACLVPVVLGIIWIGIYPGPLLRTTEASVKQLMAQVHAGYRAEGPAISHQ
                     PSAIRLKQGEGTPAVVMTSAMPDVAGEGRGGH"
     gene            complement(2421..>3839)
                     /gene="nuoL"
                     /locus_tag="AC16G0_09015"
     CDS             complement(2421..>3839)
                     /gene="nuoL"
                     /locus_tag="AC16G0_09015"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIR5099788.1"
                     /translation="RKSAADAGKKAFIVNRVGDFGFGLGVMLLWTTLGSLEYAQVFAA
                     APKQLVAGGGLPTAITLLLFLGATGKSAQLPLYVWLPDAMEGPTPVSALIHAATMVTA
                     GVYMVARSAPLFNLAPLSMEVVAWIGGLTAVFAATIALVQNDIKRVVAYSTISQLGYM
                     FLGCGVGAYAAAIFHLMTHAFFKALLFLGSGSVIHGLSGEQDLRKMGGLRRKLPITAW
                     TFLIGALANAGIIPLAGFWSKDEILFGAMSSGHTLLFLLGAGGACLTAFYMLRCYYLA
                     FEGESRVEAEAAHHLHESPPVMTVPLIILAIFAIGVGFVGVPPEHGAYHQLVEPIFAV
                     AGGAEAHAPAGGALPVAVFSLAVAVLGIWIATRFYLWRPEIPGRLTASWPGVYRVLLN
                     KYYVDELYDWLFVQPIFRGALWLWRRFDEPVIDGSVNGLGAIVRVGSGLLRRIQTGYV
                     GSYALSFIVGVVAILGYLAFWR"
BASE COUNT          800 a         1237 c         1271 g          531 t
ORIGIN      
        1 atcccgcggc tttcggcccg accgacatga aagcggtgac cgccgtcgga gccccttcgt
       61 acacgtcggg ggtccacatg tgaaagggca ccgaggccac cttgaacccg aacccgatga
      121 tcaggaggcc catgccgacg accaggagcg tgagattatg caccttcccc ccggccaaag
      181 cctccgccac ccgcttgaga ttgatgctcc ccgtggcgcc gtagaccagc gcgatgccgt
      241 agagcacgaa gcccgaggcg aaggccccca ggagcaggta tttcaaggcc gattcgttgg
      301 acttgagctg ctcccgcaga aagcccgcca ggatgtaaag cgagatggag agggtctcca
      361 gacccaggaa gatggtcatc aggtccaggc tgctggccat gatcatcatg ccgagggtgc
      421 tgaagaggag cagggcgtag tactctccgt gatccatgtc cacgctgtgg atgtagggga
      481 tggaaatcaa gagcgtcagg cccgccacca agagaaagag caggttgaag aagaaggcga
      541 actcatccgc ggccagcatc cccccgaaga cgatctcggt ctggccatgc cgcaagagct
      601 gagccacggc tgccgccagg atcccggcga gactcaggac cgcgagtcgg tcgcgccgct
      661 cgggcggggt caggggatcg aggatcaaca ccaagagccc cgccgcggtg atgagcagca
      721 tgggcagcat ggcaccccag gagatggagg gcaggctaat gtccaccgcg gccctccccg
      781 gcgacgtccg gcatcgcgct cgtcatcacg actgccggag taccctcccc ctgcttcaac
      841 ctgatggcgg atggctgatg gctgatggcg ggtccttccg cccgataccc ggcatggacc
      901 tgggccatga gctgcttcac cgaggcctcc gtcgtccgga ggagcggccc cgggtagatc
      961 ccgatccaga tgatgcccag gaccacaggg accaggcagg cgatctcccg cggggtcagg
     1021 tcgatcaggc cgcggttctc ttccttggtc agcgtgccga acatcacgcg ctggaacatc
     1081 cagagcatgt acaccgcggc caggatgatg cccgcggtcc ccagcaccgc gtagaggatg
     1141 ttcacctgga aggtgccgac caggatcagg aattccccca cgaacccgtt caggcctggc
     1201 aggccgatgg aggacagcgt gacgatcatg aaaaaggccg tgaaggccgg gatcacccgg
     1261 gccaggcccc cgaactcctc gatcagccgg gtgtgccgcc gctcgtaaat catccccacg
     1321 aggaggaaca gcgccccggt ggacaggcca tggttgatca tctggaggat cccgccctcc
     1381 agcccctggc tggtgagggc aaagatcccg agcatcacaa agcccagatg gctcacggag
     1441 ctgtaggcca ccagcttctt cagatcgggc tgtaccatgg acaccagggc cccgtacagg
     1501 atcccgatga tggccagcca ggagaacagc ggcgtgaaga atcgggtggc ctccgggaac
     1561 aggggcaggg cgaagcgcag gaacccgtag gtccccatct tcaggaggac ccccgccagg
     1621 atcaccgagc cggccgtggg cgcctcgacg tgggcgtcgg gcagccaggt gtggaagggg
     1681 aacatgggca ccttgatggc gaaggccact gcaaaggcca ggaacagcca ggcctgtgtc
     1741 gcaaagggca gcgtcagctc cgcggagatc ttcagcaggt cgaaggtgaa cacggcgccc
     1801 ggctgcccgc cgtgcaggta gtacaggctg atgatcccca ccagcatcag gacgccgccc
     1861 gccatggtgt agaggaaaaa tttgacggtg gcgtagatcc ggttgggtcc tccccagacg
     1921 ccgatcagga agtacatcgg gatcagcatc ccctcccaga agagatagaa caggaccagg
     1981 tccagggcca cgaagacccc cagcatgccg gtttccagga ggagcatgca gatcatgtac
     2041 tccttcaccg aggtggtgat ggctgtgaag gaggagagga tggcgatggc cgtgaggaac
     2101 gtcgtgagga ggaccaggag caggctgatc ccgtccacgc cgacgaagta ctggacgttg
     2161 aaacccgcga tccagggccg ctgttccacg aactgcatcc caggaacgtt aagctggaag
     2221 cccgagtaga gcaggaggga caccaggaac gtgaggagcg acacgccaaa ggccagcccc
     2281 ttgatggcgc cctcgtgccg gcggtccaac cccagcaaga gcaccgcccc cgcgcacggc
     2341 aggaagatca cgatggagag gatgggaaag tcactcatcg ttcgcggccc acgctattcc
     2401 ccattgcagc tcgtccctgt tcatcgccag aacgccagat aacccaggat ggccacgacc
     2461 cccacgatga agctcagggc atagctcccc acgtacccgg tctggatgcg gcggagcagg
     2521 ccgctcccca cccggacgat ggctccgagg ccgttcaccg agccgtcgat caccggctcg
     2581 tcgaatcgcc gccagagcca gagggcccca cggaagatgg gctggacgaa gagccagtcg
     2641 tacagctcgt ccacatagta cttgttcagc aggacccggt agacccctgg ccagctcgcg
     2701 gtgagtctgc ccggaatctc cgggcgccag aggtagaatc gggttgcgat ccagatcccc
     2761 agcacggcca cggcgaggga gaagacggcg acaggcaggg ccccgcctgc cggcgcatgc
     2821 gcttccgccc ctccggcgac ggcgaagatc ggctccacga gctggtgata ggcgccatgc
     2881 tccggcggga cgccgacgaa gcccacgccg atggcgaaga tcgccagaat gatcaggggg
     2941 accgtcatga cgggcggcga ctcgtggaga tgatgcgccg cctctgcttc gacccgggac
     3001 tccccctcga aggccaggta gtagcagcgc agcatgtaga aggcggtgag acaggcgccg
     3061 ccggccccca gcaggaagag cagcgtgtgc ccgctgctca tcgcgccgaa caagatctca
     3121 tccttggacc agaaccccgc cagggggatg atgcccgcgt tggccagggc accaatcaga
     3181 aacgtccagg cggtgatggg gagcttccgc cgcagccctc ccatcttgcg caggtcctgc
     3241 tcccccgaga ggccgtggat gaccgagccg gatccgagga acaggagcgc cttgaagaag
     3301 gcgtgggtca tcaggtggaa gatggccgcg gcgtaggccc ccaccccgca gcccaagaac
     3361 atgtagccga gctgggagat ggtcgagtag gcgaccaccc gcttgatgtc gttctggacc
     3421 agggcgatgg tggccgcgaa gacggcggtg aggcccccga tccaggccac cacctccatg
     3481 gagagagggg ccaggttgaa cagcggggcg gagcgggcca ccatgtacac cccggcggtc
     3541 accatggtgg cggcgtggat cagggccgaa accggcgtgg gcccctccat ggcatccggc
     3601 agccagacgt agagcgggag ctgggccgat ttccccgtgg cacccaggaa cagcaggagc
     3661 gtgatggccg tcgggaggcc gccgcccgcc acgagctgct tgggcgcggc cgcgaagacc
     3721 tgggcgtact ccaggctccc cagggttgtc cagaggagca tcacccccag accgaagccg
     3781 aagtctccga cgcggttgac gatgaacgcc ttcttccccg catctgccgc cgactttcg
//
LOCUS       DBNGZC010000515         3450 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2098913, whole genome shotgun sequence.
ACCESSION   DBNGZC010000515 DBNGZC010000000
VERSION     DBNGZC010000515.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3450)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3450)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3450
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2098913"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="AC16G0_09020"
     CDS             complement(<1..512)
                     /locus_tag="AC16G0_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099789.1"
                     /translation="MRRVLWLIGVGFLFVIAAGIQRPPAAAAAAQTKVVIGLNSDVRS
                     FDPINTLDTTTDRVISHIYEYLYTRDRSMKLVPLLAESARTLDDLNWKIKLRKNVKFS
                     DGEPFNADTVKANIEYIVKKENNAARRVRIDLVKSVTVVDEHTVRITTEKPFPTLLEG
                     LTEIYMVPIRA"
     gene            complement(669..1514)
                     /locus_tag="AC16G0_09025"
     CDS             complement(669..1514)
                     /locus_tag="AC16G0_09025"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5099790.1"
                     /translation="MELRVSGTTLHCEIVGDGVPCLCLHGGPGSDSSGLRPALEPLAK
                     RLGLRLIFYDHRGHGRSEWVPVEQCTQDQLVADIDAVRQTLGLGRVHVLGISWGGFLG
                     LMYAARYPDSVRALAVVGAAASHEFMRRAEDNARRQATPEQWTAYRALWDGSLPDDES
                     LRRAFETIRPLYFFEKRLAAGSNAARAATRYRIAVRNFVIQHEYPAYDCRTELSRIRC
                     PTLVAVGRHDWICPIEFAEEIHKLVPGSKLAIFERSGHSPQIEESEAFAGQFGAFIAT
                     TAEAT"
     gene            complement(1519..3147)
                     /locus_tag="AC16G0_09030"
     CDS             complement(1519..3147)
                     /locus_tag="AC16G0_09030"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HIR5099791.1"
                     /translation="MTRSSLITALVLLMGLLDPTPGVTGGRDMVIGLSGEATSLNPVV
                     ATDSMSYIVEWPLFDSLLELDQNLNVRPLLAESWEMSKDGLSYTFKLKKGVKWHDGAP
                     FTARDVAFTFYSVLNPKVTTPHRAYFDALAGFPELTAKDNPKRPEELAVRPIEILDDY
                     TVRFHLRYPSGSFLAVLVNPRAGIIPEHLLKGVDLNTAEFNRRPVGTGPFKFVEWRRG
                     ERVVMEANDQYHGGRPALNRLIFRIIPDSVVLLEDLRAGGVDFIENPPLTEVAHLKQM
                     PGLKVLVADNTSYTYFGWREDLEPFTDIRVRRALNHAIDVGSIVRETLQGYAAIATGQ
                     FPPASWAFDPSVKPYSYDPNRSKALLAEAGFKPGPDGILVKDGKRFSFSIRHDQADQR
                     VKDTAVIIQEYLKRIGVEARLEPLDWPTFVKKLFASDFEGIVVGWTNHHDPDPFAYTI
                     WHSSQWKGRNFAHYKNPRVDELLEKARRTAVVAERKRYYGEFSKTLMEDAPYVFLYFP
                     QQVYVTRQGYEGFVPIPTYAGIYQSLKSVRWTGK"
     gene            complement(3177..>3450)
                     /locus_tag="AC16G0_09035"
     CDS             complement(3177..>3450)
                     /locus_tag="AC16G0_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012946554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099792.1"
                     /translation="NALPVIIVQASLLLGQAILIESALSYLGLGAQPPLPSWGNMIVE
                     GRQFLASAWWIATFPGVAIFATVLGFNLFGDGLRDILDPTLRSGSS"
BASE COUNT          609 a         1079 c         1135 g          627 t
ORIGIN      
        1 gccctgatgg gcaccatgta aatctccgtg agcccttcca gcaaggtggg gaagggcttc
       61 tcggtggtga ttctgacggt atgctcgtcc accaccgtga ccgacttcac caggtcaatg
      121 cgaacccgtc tggccgcgtt gttctccttc ttgacgatgt actcgatatt cgccttcacc
      181 gtatcggcat tgaagggttc cccgtcgctg aacttcacgt tcttgcggag cttgatcttc
      241 cagttcaggt cgtccagggt ccgcgccgac tccgccagca gcggaacgag cttcatgctg
      301 cggtcgcggg tgtacaagta ctcgtagata tgactgatga ctcgatcggt ggtggtatcc
      361 agggtgttta tcgggtcgaa gctgcgcacg tccgagttga ggccaatcac gaccttcgtc
      421 tgggccgccg ccgcggccgc gggaggccgc tggatcccgg cagcgatcac gaagaggaat
      481 cccacaccga tcagccagag gaccctacgc atcgcgcgct cctttctgag aactcgtggg
      541 tatcacggcc gccagccaat gcccctggtc gattcctggg ggccgaaggt gacgcctcgc
      601 gttgggacgg gagcccatgg caccatggca gcggacccgt cgaggcgtct ggggtgccca
      661 aggccccatc acgtggcttc ggctgtcgtg gcgatgaagg ccccgaactg cccggcaaag
      721 gcctcggact cctcgatttg cggcgagtga ccgctccgct cgaagatcgc caacttcgag
      781 cccggcacca gcttgtggat ttcctcggcg aactcgatgg gacagatcca gtcgtgccgc
      841 cccaccgcga ccagcgtggg acagcggatg cgggaaagct ctgtgcggca atcgtaggca
      901 gggtactcgt gctggatgac gaagttgcgg accgcgatcc ggtatcgggt cgcggcccgg
      961 gccgcattgc tccccgccgc gagacgcttc tcgaaaaaat agagcgggcg gatcgtttcg
     1021 aaggcccggc ggagactttc gtcatccggc agagacccat cccacagggc acgatacgcc
     1081 gtccactgct ccggagtcgc ctgccggcgg gcgttatcct ccgcccgacg catgaactcg
     1141 tgactggccg cggcgccaac gacggcgagg gcgcgaacgg aatcggggta ccgggccgca
     1201 tacatgaggc ccaagaagcc gccccagctt atccccagga cgtgcacgcg cccgaggccg
     1261 agcgtctgac ggacggcatc gatgtccgcc acgagctggt cctgggtgca ctgctcgacg
     1321 ggaacccatt cggaccgtcc atgtccgcgg tggtcgtaga agatcaggcg gaggcccagc
     1381 cgcttggcca gcggctccaa ggccgggcgc agacctgacg agtcgctgcc cggccctcca
     1441 tgcaggcaga ggcagggcac cccgtcgccc acgatttcgc agtggagtgt tgtcccgctg
     1501 acccgaagct ccatggacct actttccggt ccagcgcacc gacttcaatg attggtagat
     1561 tcccgcgtac gtcgggatgg ggacgaagcc ctcgtatccc tggcgcgtca cgtacacctg
     1621 ctgcgggaaa tagaggaaga cgtagggcgc gtcctccatc agggtctttg agaactcgcc
     1681 gtagtaccgt ttccgctcgg cgacgacggc cgtgcgccgg gctttctcca gcaactcgtc
     1741 gacccgcgga ttcttgtagt gggcgaagtt ccggcccttc cactggctcg aatgccagat
     1801 ggtataggcg aagggatcgg gatcgtggtg attggtccag ccgacgacga tgccttcgaa
     1861 gtccgaggcg aagagcttct tgacaaaggt gggccaatcc agcggctcca gcctggcctc
     1921 gactccgatc cgcttcaagt actcctggat gatcaccgcg gtgtccttca cgcgttgatc
     1981 cgcctggtcg tgtcgaatcg aaaagctgaa gcgcttgccg tccttgacca ggatgccgtc
     2041 ggggcccggc ttgaagcccg cttcggccag caaggccttc gaccggttcg gatcgtagct
     2101 gtagggcttg acgctcgggt cgaaggccca actcgccggc gggaactgcc cggtggcgat
     2161 cgcggcgtac ccctggaggg tttcgcgcac gatgctcccg acgtcgatgg cgtggttgag
     2221 cgcccggcgg acgcggatgt cggtgaaggg ctcgaggtct tcacgccagc cgaagtaggt
     2281 gtacgaggtg ttgtcggcga cgagcacctt gagcccgggc atctgcttga gatgcgccac
     2341 ttccgtcagc ggcgggttct cgatgaaatc gacaccgccc gcccgaaggt cttccagcag
     2401 gaccacgctg tccgggatga tgcggaagat cagccggttc agggccggtc gtcccccatg
     2461 atactggtcg ttggcctcca tcacgacgcg ctcaccccgc cgccactcga cgaacttgaa
     2521 ggggccggtg cccacgggtc tccggttgaa ctcggcggtg ttgaggtcaa cacccttgag
     2581 gagatgctcg gggatgattc cggcccgggg gttgacaagg acggcgagga atgagccaga
     2641 gggatagcgg aggtggaagc gcaccgtgta atcgtccagg atttcgatcg gtcgcaccgc
     2701 cagctcttcg ggccgcttgg ggttgtcctt ggcggtcagc tccgggaacc cggccagggc
     2761 gtcgaagtat gcccggtgcg gcgttgtcac cttggggttg aggacggagt agaaggtgaa
     2821 ggcgacgtcg cgcgccgtga acggcgcccc gtcgtgccac ttcacgccct ttttgagctt
     2881 gaaggtatag ctgagcccgt ccttcgacat ctcccaggac tcggccagga gaggtcggac
     2941 gttgaggttc tggtccagtt ccagcagact gtcgaagagc ggccattcca cgatgtagga
     3001 catactgtcc gtcgccacga ccgggttgag cgaagtcgcc tccccgctga ggccgatgac
     3061 catgtccctt ccgccggtaa cccccggcgt cggatcgagc aaccccatga gaagcacgag
     3121 cgcggtgatc agactggacc gtgtcatggg tatcccccct ttccatgacg ccgtggtcat
     3181 gacgagcccg accgcagggt cggatcgagg atatcgcgga gaccgtcgcc gaagaggttg
     3241 aagccgagga ccgtggcgaa gatcgccacg ccgggaaagg tggcgatcca ccaggccgaa
     3301 gccaggaatt gccgcccctc gacgatcatg ttgccccaac tgggcagtgg cggttgggcg
     3361 cccagcccca ggtaactgag tgccgactcg atgagaatgg cctgaccgag cagcaggctc
     3421 gcctggacga tgatgaccgg tagcgcgttg
//
LOCUS       DBNGZC010000516         2414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_141642, whole genome shotgun sequence.
ACCESSION   DBNGZC010000516 DBNGZC010000000
VERSION     DBNGZC010000516.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_141642"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /gene="glk"
                     /locus_tag="AC16G0_09040"
     CDS             complement(<1..886)
                     /gene="glk"
                     /locus_tag="AC16G0_09040"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017739781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HIR5099793.1"
                     /translation="MILAGDIGGTNTRLAWVAPDGERLTVVQETAFPSREYASLEAVL
                     ERFLSAQRYPVERACFGVAGPVRHGRVDATNLPWVVDSRSLTGQLGLEHVGVINDLVA
                     NAHGISALEAKDFTLLNPGAADAQGNVAVISAGTGLGEAGLYWDGTRHRPFATEGGHA
                     TFAPRGALEMDLLRHVSTQFQHVSVERVLSGPGLLLIYRFLRDTGHGEEPAWLAEELR
                     QRDPPAVIAETAMESKNDLCIQALDLFVSIYGAEAGNLALKVMATGGVFLGGGIAPKI
                     LPKLQDPGFLEAFGAKGRM"
     gene            complement(1262..>2414)
                     /locus_tag="AC16G0_09045"
     CDS             complement(1262..>2414)
                     /locus_tag="AC16G0_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HIR5099794.1"
                     /translation="HVFLGEPSIGGRYSALSDFGMVPAAVMGLDVRQFLDRAEEMVHA
                     CASCVPPEENPGAVLGTILGVLGKSGRDKVTLIASPGIRDLGAWLEQLLAESTGKQGK
                     GLIPVDREQLGPPEAYGSDRLFVYVRLGPAPDPKQDAAVGALEQAGLPVVHIGVVDPY
                     DLGQEFLRWEIATAVAGSILGINPFDQPDVEASKVATRDLTGEYERTGSLPAETPLFD
                     GDGIRLFADGRNADTLNQRAGSDHTLAGYLRAHLGRLLAGDYLVLLAYLEMTDAHESK
                     LQAMRHAIRDSKRVATCLGFGPRFLHSTGQAYKGGPNTGVFLQITCEDAADLPVPGQR
                     YSFGVVKAAQARGDFRVLAERGRRALRVHLGDVETGLRTLGVAVERALG"
BASE COUNT          375 a          879 c          794 g          366 t
ORIGIN      
        1 tcatccgccc cttggcgccg aaggcttcca ggaacccggg gtcctggagc ttcgggagaa
       61 tcttgggggc gatcccgccc ccgaggaaca cgccgcccgt ggccatgacc ttcagggcca
      121 ggttgccggc ctcggcaccg tagatcgaga cgaacaggtc cagggcctgg atgcagagat
      181 cgttcttgct ctccatggcc gtttcggcga tgacggcggg gggatctcgc tggcgcaact
      241 cctcggcaag ccaggctggc tcctcgccgt gtcccgtgtc ccgcaggaac cggtagatca
      301 ggaggaggcc cggccccgac agcacgcgct cgacgctcac gtgctggaac tgcgtggaca
      361 cgtgacgcag cagatccatt tccagcgctc cccggggcgc gaaggtggca tgcccaccct
      421 ccgtggcaaa gggccggtgg cgtgtgccgt cccagtaaag ccccgcttcg ccgagcccgg
      481 tccccgcgga gatgacggcc acgttgccct gggcgtccgc ggcccccgga ttcaggagcg
      541 tgaagtcttt tgcctccagc gcggaaatgc cgtgggcgtt ggccaccagg tcgttgatca
      601 cccccacatg ctcgagaccc aattgcccgg tcaggctccg ggagtccacc acccagggca
      661 aattggtcgc gtcaacccgg ccatggcgga cagggcctgc gaccccgaag caggcgcgtt
      721 caacggggta gcgctgtgcc gagaggaacc gctccagcac cgcctcgagg ctggcgtact
      781 cccggctggg aaaggcggtc tcctgcacga ccgtgagacg ctcgccgtcc ggcgcgaccc
      841 aggccagccg ggtgttggtc ccgccgatgt ctcctgccag gatcatggca cgctcccccg
      901 caccttccgg ccgctctgct ccggcggctt gccggccact cgactcaacc gccaagaacg
      961 atccacccca ccgctgttcc ggagttcggg agcggccgat ccgtctgagg atcgggcggc
     1021 tgaggatggc tgactggtgt acagtcccag agattcgccg tataagtcag ggcctcagtg
     1081 ccaccccgag gacgctgcgc cacacccatc ctgcggctgg gtaccccccg gccgggtccg
     1141 gcccggaggc ccccgttctg tgggggccga cccggagcaa ggccgggcgg ttcgcgacga
     1201 ggcgcctggc ggcggagggg cggctttgtg gcccggcaat tggactccag ggaataggac
     1261 actaccccag cgcccgctcg accgccacgc ccagcgtgcg caagcctgtc tccacgtccc
     1321 cgaggtgcac ccggagggca cggcggcctc gctccgccag aacccgaaag tcgccgcgcg
     1381 cctgggcagc cttgaccacg ccgaaggagt agcgctgccc cggcaccggc agatccgcgg
     1441 catcctcgca ggtgatctgg aggaacaccc ccgtgttggg tccgcccttg tacgcctgtc
     1501 cggtggagtg caggaagcgc gggccgaatc ccagacaggt cgcgacccgt ttgctgtccc
     1561 ggatggcgtg gcgcatggcc tgcagcttgg actcgtgggc atcggtcatt tccagatacg
     1621 ccaagagcac gaggtaatcc cccgccagga gccgccccag gtgtgcccgg aggtatcccg
     1681 cgagggtgtg atcgctgccc gcacgttggt tcagtgtgtc ggcattccgc ccgtccgcga
     1741 acagccggat gccgtccccg tcgaataacg gggtctccgc ggggagcgag ccggttcgct
     1801 cgtactcgcc ggtcagatcc cgggtcgcca ccttgctggc ctccacatcc ggctggtcga
     1861 aggggttgat ccccaggatc gagcccgcca ccgccgtcgc gatctcccag cggagaaact
     1921 cctggccgag atcgtagggg tccaccaccc cgatgtgcac cacggggagc cccgcctgtt
     1981 ccagggcccc cacggcggcg tcctgtttcg gatcgggggc tggccccagt cgcacgtaga
     2041 cgaacaaccg gtcggacccg tacgcctccg gcgggcccaa ctgctcccga tccacgggga
     2101 tgagtccctt cccctgcttg cccgtggact ccgccagcag ttgctccagc cacgcgccca
     2161 ggtcccggat gccaggcgag gctatcaggg tcaccttatc gcgcccgctt ttccccagga
     2221 cacccaggat cgttcccagg accgcgcccg ggttctcctc gggcgggaca caggaggcgc
     2281 aggcgtgcac catctcttcg gcccggtcca ggaactgccg cacgtccaga cccatgacgg
     2341 ccgccggcac catgccaaag tccgacagcg ccgagtagcg cccgccgatg gacggctccc
     2401 cgaggaagac gtgc
//
LOCUS       DBNGZC010000517         2045 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1805177, whole genome shotgun sequence.
ACCESSION   DBNGZC010000517 DBNGZC010000000
VERSION     DBNGZC010000517.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1805177"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="AC16G0_09050"
     CDS             complement(<1..1136)
                     /locus_tag="AC16G0_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR5099795.1"
                     /translation="MAAVIKGNYPLITFEADEGGATDWYPSVIAAQGDRLAFGLRAQA
                     VQHEPGWEVLRSFKRLLQDNAPHSLWKVGGVELPAIEWLRLFLTALHQDLLRHSNLDA
                     GRRERLEVMVGVPANANSNQRFLTLEAFRQAGFEPIGMLNEPSAAGIEYAHGYRTAEV
                     ARRREHVVVYDLGGGTFDAAVICMSGNQHDVIASEGISRLGGDDFDVALLELALSQAR
                     SAAPDPGVSRSRLLDLCREAKESINPNSRKVVVDFGQVKPGLGDVMVPIPQFYDRCAP
                     LIHRTIQATEAAMQAALGKADGELPGVVAVYLVGGSCELPVLARALRERFGRRVRRSP
                     YPPAATAIGLAIAADQEGGYTLTERFTRHFGVWREGESGEKVVF"
     gene            complement(1393..1887)
                     /locus_tag="AC16G0_09055"
     CDS             complement(1393..1887)
                     /locus_tag="AC16G0_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HIR5099796.1"
                     /translation="MKRILFVCTGNICRSPMAEALLRDALQQDPELRSQGIEVRSAGT
                     SGWDGAPATDLAVRAMMSLGLNLRGHRGRQLTRELLNWAECLLTMEARQRDWIQHAYP
                     DAAAKVFNLADCAGDSGDVEDPYGGDEAEYAQCARRLCALIPGLVTHLRSRLNRGAGP
                     SPPG"
BASE COUNT          373 a          707 c          620 g          345 t
ORIGIN      
        1 tcgaacacga ccttctcccc tgactcgcct tcgcgccaca cgccgaagtg acgggtgaag
       61 cgctccgtga gggtgtatcc accctcctgg tcggcggcga tcgccaggcc gatcgcggtc
      121 gcagccggcg gatacggaga ccttcggaca cgcctcccga accgctcccg caacgcccgg
      181 gccaggacgg gcagctcgca ggagccgccg acgagataca cggctaccac ccccggcagc
      241 tcaccgtcgg ccttgcccaa cgccgcctgc atggccgcct cggtggcctg gatggtccga
      301 tggatgaggg gggcgcagcg gtcgtagaat tgaggaatcg gcaccatcac gtcgcccagg
      361 cccggcttca cctgaccgaa atccaccaca accttgcggc tgttgggatt gatgctctcc
      421 ttggcctccc ggcacaaatc caaaagacgc gaacgggaga cccccggatc cggagccgcg
      481 gaccgcgcct gggaaagcgc cagctccaga agcgccacgt cgaaatcatc tccccccagc
      541 cgggagatgc cctcgctggc aatgacgtcg tgctggttcc cggacatgca aatgaccgcg
      601 gcgtcgaacg tgccgccacc gaggtcgtac accaccacgt gctcgcgccg ccgcgccacc
      661 tccgccgtac ggtagccatg agcatactcg atgccggcgg ccgacggctc attgagcatc
      721 ccgatcggct cgaaccctgc ctgccggaag gcctccagcg tgaggaagcg ctggttgctg
      781 ttggcgttgg ccgggacccc aaccatcacc tcgagccgct cacggcgacc cgcatccagg
      841 ttgctgtgcc gcagcaagtc ctggtgcagt gccgtgagga acagcctcag ccattcgatg
      901 gcgggaagct cgaccccgcc cactttccag agagagtggg gtgcgttgtc ctgcaacagc
      961 cgcttgaagg agcggagcac ttcccagccc ggctcgtgtt gcacagcctg ggcacgcagg
     1021 ccgaaggcca gccggtcgcc ctgggccgcg atcaccgagg gataccaatc cgtcgcgccc
     1081 ccttcatcag cctcgaaggt gatcagcgga tagttgccct tgatgacagc agccacccgt
     1141 gtccgggtcg ttccgaagtc aattccaaga cgcatccttt tggtcctctg gtttcggggg
     1201 gtcatttccc cagggtcccc gccgacctgt ggctgcagcc aagggcaggg ttgggcgtta
     1261 ggataccatg acgcctcttg taaggggtag cccttctcgg gttaaccggg cgatcgagtc
     1321 gggccatccc cctccgactc ggctcggcct acccacctca ccgacatcat cgccaagagg
     1381 acggcgaagg ggtcaaccag gcggtgacgg gccggcgcca cggttgagcc gggagcgcag
     1441 gtgggtgacc agcccaggga tcaaggcaca aagccgacgg gcacactggg catactcggc
     1501 ctcatctccc ccatagggat cctcgacatc gccggagtcg ccggcacagt cggcaaggtt
     1561 gaagaccttg gcagcggcat cgggataggc gtgctgtatc cagtctcgct gccgcgcctc
     1621 catcgtcaag aggcattcgg cccaattcag caactcccgc gtcaattgac gccctcgatg
     1681 accgcggagg ttgagtccaa gactcatcat cgcccggacc gccagatccg tggccggcgc
     1741 cccatcccag ccgctcgtcc cggccgaccg gacctcgatg ccctggctcc ggagttccgg
     1801 atcctgctgg agcgcgtccc gcaacagagc ctcagccatc gggctccgac agatatttcc
     1861 cgtgcacacg aagagaatcc gcttcattga tcgcctcagg caatgtgaag tgacttcgcg
     1921 gaatgatgct gtggggttcc tcgatgcgag cctcctcgga aaattatact cgtcctgcgt
     1981 ggggttgccg atgcataatg tggcgttctt tctgcgatcc atggcaaaac gtaccataat
     2041 tcctg
//
LOCUS       DBNGZC010000518         3012 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2391811, whole genome shotgun sequence.
ACCESSION   DBNGZC010000518 DBNGZC010000000
VERSION     DBNGZC010000518.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2391811"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..>3012
                     /gene="secA"
                     /locus_tag="AC16G0_09060"
     CDS             319..>3012
                     /gene="secA"
                     /locus_tag="AC16G0_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR5099797.1"
                     /translation="MIKHLIHKIIGTKNERELKRLRPQVEAINALEPEVQRLKDADLK
                     VRTATFKERVATVLASINGGAEDATEDAKARNDALKAILDELLPEAFAVCREAARRVL
                     NMRHFDVQLIGGMVLHEGKIAEMATGDGKTLVATLPVYLNALSGRGVHVVTVNAYLAK
                     RDTQWMGPIYHALGLSVGVLQHEASFLFDPTSRSADMRLANLRACTRQEAYGADVTYG
                     TNSEFGFDYLRDNMKFSAAEYVQRELHYAIVDEVDNILIDEARTPLIISGASDESTDK
                     YYAIDRIIPRLKRAATITSGKLHEIEATAAGDFIVDEKAKTVALTETGVLTVEKLLGV
                     KNLYEPANIEIVHHVQQGLRAHSLYKRDVDYVIKDGEVIIVDEFTGRLMPGRRWSDGL
                     HQAIEAKEKMKIQRENQTLASVTIQNYFRMYGKLAGMTGTADTEAAEFAQIYNLDVMV
                     IPTNRPLIRVNQNDMVYKTNKEKLNAVADEIAELYKVGRPVLVGTTSIEKSEAVSSLL
                     KRRGIPHQVLNAKPMYAEREAEVVAQAGRFKAVTISTNMAGRGTDILLGGNPKFLATE
                     LVRRGEVGHDGLDPGEYEKVLAEVRDMQRYGLLETDDPENYAAALARVRKQIEAEHKK
                     VVELGGLHILGTERHEARRIDNQLRGRAGRQGDPGSSRFYLSLEDDLLRLFAQERIAK
                     IMEKLGMEEGEPIEHPWVTRAIETAQKRVEAHNFEIRKHLLEYDDVMNTQRKVIYGER
                     RQLLEGTDVREVIEGMIEEVLEESVGVYVDEDAYPENWNLAGLAEAMKLQFDVEAAWS
                     KEQVEALTPVVLKEELQEKVKTAYRKKEEQVGSDMMRFLERMAMLQVVDNQWKDHLLA
                     MDHLKEGIGLRGYGQKDPLVEYKREGFEMFEAMEARIAKSTI"
BASE COUNT          634 a          891 c         1024 g          463 t
ORIGIN      
        1 ccggagtgaa attcgctcgc gagggtgaca acgggggtca gtcgtagctc tccgatctcg
       61 attaccacaa cagccccgag gactcctcct cggggctttt cttttgtgcg ggtcgcaggg
      121 gagaggaccg gagcgggcaa tcgagaatgg ggcggcttgg cctgacggca accgggcagg
      181 gaaatggcca ttcctccggg gccccgggtc gcccgatcgc ctcttggtct ggggggaatt
      241 gaattggcgt gcctgcgctt tgtctgctaa gatacgtcgg cctgcaaaga ccggaagcga
      301 aaggacggga gagtcgggat gatcaagcat ctcatccaca aaatcatcgg cacaaaaaac
      361 gagcgggagc tgaagcgcct gcgcccgcag gtcgaggcca tcaacgccct cgagccggag
      421 gtccagcgcc tgaaggatgc ggacctgaag gtgaggacgg cgacgttcaa ggagcgcgtg
      481 gccactgttc tggccagcat taacggtggg gccgaggacg ccacggagga tgccaaggcc
      541 aggaacgacg ccctcaaggc catcttggac gagctcctcc ccgaggcgtt tgccgtctgc
      601 cgcgaggcgg cccggcgcgt gctgaacatg cgccatttcg atgtccagct catcgggggc
      661 atggtcctgc atgaggggaa gatcgccgag atggccacgg gcgatggcaa gacattggtc
      721 gccacattgc ccgtgtacct gaatgcactc tcgggtagag gcgtccacgt cgtcacggtc
      781 aacgcttact tggccaagcg ggacacccag tggatgggcc ccatctacca cgcattgggt
      841 ctgagcgtgg gcgtcctgca gcacgaggcc tcctttctct tcgatcccac gtcgcggtcc
      901 gccgacatgc gattggcgaa tctccgagcg tgcacccggc aggaagccta tggggctgac
      961 gtcacctacg ggaccaacag cgagttcggg ttcgactacc tgcgcgacaa catgaaattc
     1021 tcggctgcgg agtatgtgca gcgcgagctc cactacgcca tcgtggacga ggtggacaac
     1081 atcctgatcg acgaggcccg gaccccgctc atcatctccg gggcctcgga cgagtcgacg
     1141 gacaagtact acgccatcga ccggatcatc ccccggttga agcgggccgc taccatcacc
     1201 agcggcaagc tgcatgagat cgaggccacc gccgcgggtg acttcatcgt ggacgagaag
     1261 gcgaagaccg tggccctcac cgagaccggc gtgctcacgg tggagaagct cctgggggtg
     1321 aagaacctct acgagccggc caacatcgag atcgtccacc acgtccagca gggtctgcgg
     1381 gcgcacagcc tgtacaaacg ggatgtggac tacgtgatca aagacggcga ggtcatcatc
     1441 gtggatgagt tcaccgggcg cctgatgccg gggcgccggt ggagcgacgg tcttcaccag
     1501 gccatcgagg ccaaggaaaa gatgaagatc cagcgggaga atcagaccct cgcctccgtc
     1561 accatccaga actacttccg catgtacggc aagctggcgg gcatgaccgg caccgccgac
     1621 accgaggcgg cggagttcgc ccagatctac aacctggacg tgatggtcat cccgaccaac
     1681 cggccgctca tccgcgtgaa ccagaacgac atggtgtaca aaaccaacaa ggaaaagctg
     1741 aacgcggtgg cagacgagat cgccgagctg tacaaggtgg ggcgacccgt cctggttggg
     1801 acgacctcca tcgagaagtc cgaggcggtg agcagcctgc tcaagcggcg aggaatcccc
     1861 caccaggtct tgaatgccaa gccgatgtac gccgagcgcg aggccgaggt cgtggcccag
     1921 gccgggcgct tcaaggcggt gaccatctcc accaacatgg cgggccgcgg gaccgacatc
     1981 ctcctggggg gcaacccgaa gttcctggcg actgagctgg tccgccgggg agaggtgggc
     2041 cacgacggcc tggatcccgg cgagtacgag aaggtcctgg ccgaggtgcg ggacatgcag
     2101 cggtacggcc ttctggagac cgatgaccct gaaaactatg ccgcagcgct ggcgcgggtc
     2161 cggaagcaga tcgaggccga acacaagaag gtggtcgagc tgggcggctt gcacatcctg
     2221 gggacggagc ggcacgaagc ccgccggatc gacaaccagt tgcgtggtcg cgccgggcgc
     2281 cagggcgacc cgggctcgtc ccgcttctac ctctccctgg aggacgacct cctgcgcctc
     2341 ttcgcccagg agcgcatcgc caagatcatg gagaagctgg gcatggagga gggggagccg
     2401 atcgagcacc cctgggtcac gcgcgccatc gaaaccgccc agaagcgggt ggaggctcac
     2461 aacttcgaga tccggaagca cctcctggaa tacgacgacg tcatgaacac ccagcgcaag
     2521 gtcatctatg gcgagcgccg gcagctcctg gagggaactg acgtgcgcga ggtcatcgag
     2581 gggatgatcg aggaggtcct ggaagagtcc gttggcgtgt acgttgacga ggatgcgtat
     2641 cccgagaact ggaatttggc cggcctggca gaggcgatga agctgcagtt cgacgtggag
     2701 gctgcctggt ccaaggagca ggtcgaggcg ctcacccccg tggtcctcaa ggaagagttg
     2761 caggagaagg tcaagaccgc gtaccggaag aaggaagaac aggtcggaag cgacatgatg
     2821 cggttcttgg agcgcatggc catgctgcag gtggtggaca atcagtggaa ggatcatctg
     2881 ttggccatgg accacttgaa ggaggggatc ggcctccggg gctacggcca gaaggacccg
     2941 ctggtggagt acaagcgcga gggcttcgag atgttcgagg ccatggaggc gcggatcgcc
     3001 aagagcacca tc
//
LOCUS       DBNGZC010000519         3195 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1413602, whole genome shotgun sequence.
ACCESSION   DBNGZC010000519 DBNGZC010000000
VERSION     DBNGZC010000519.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1413602"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="AC16G0_09065"
     CDS             complement(<1..598)
                     /locus_tag="AC16G0_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099798.1"
                     /translation="MLDVEDIQVYYGEILALKGVSVRVGDGEIVALLGNNGAGKTTSL
                     KTISGVLRPRTGRILLEGRPIHGLPAHEIVGLGIAHAPEGRRIFNRLSVVENLEMGAY
                     LRSDAGIAEDIDRVFSLFPRLRERRLQVAGTLSGGEQQMLAIGRAIMTRPRLLLLDEP
                     SMGLAPILVEQIFQTIEEINRQGTTILLVEQNAAIALSV"
     gene            complement(591..1388)
                     /locus_tag="AC16G0_09070"
     CDS             complement(591..1388)
                     /locus_tag="AC16G0_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006631853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099799.1"
                     /translation="MAAILDVRQITKKFGGLTALNHVDYLLEEGHIASIIGPNGAGKT
                     TLFNVFTGIYQPEGGEVYFKGKPLLGLRPDQITALGICRTFQNIRLFSNMTALENVLV
                     GMHSRIAFSMASVLVRSHHFQQEEHRLWSRASGLLEVVGLVAKANEVARNLPYGERRR
                     LELARALASEPTLLLLDEPTAGMTQGEAQSLMALLRRLILEYRLTILLIEHNMRVVMG
                     VSDRVTVLDYGEKIAEGQPSEVQKNPRVIEAYLGRKRWGAPPDSAHA"
     gene            complement(1389..2654)
                     /locus_tag="AC16G0_09075"
     CDS             complement(1389..2654)
                     /locus_tag="AC16G0_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5099800.1"
                     /translation="MSALLERPWLATVLTMAVIGLSSFGVAYYPRSVLVFLLFQSSIL
                     LLYLARVRPWLKLLLGGVTLGVVMPILGTINGYYLEVATQVGIFVALSLGLNIVVGLA
                     GLLDLGYVAFYAVGAYTWAIFGSHQAGKFIPEWVATFPLGGSWFFVFLILAVCVGATA
                     GILLGLPVLRLRGDYLAIVTLGFGEVIRVLANNLDKPINFTNGPQGITPIVRPPLFFQ
                     PVLEFIGIHASPVALYPLYLYFIVLCIVLIVIQVNRRLEDSHIGRAWEAIREDEVAAQ
                     AMGVPLVRMKLMAFACGASFAGAMGAVFSAKQVFINPESFTFLESIGVLSMVILGGMG
                     SIPGAILGASVVTVLNLQVLKGLSLWLNELRNAGTIIFGYNLANLPTQLEPAKYERMI
                     FGAILILMMIFRPQGILPARRRTREFGGK"
     gene            complement(2664..>3195)
                     /locus_tag="AC16G0_09080"
     CDS             complement(2664..>3195)
                     /locus_tag="AC16G0_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5099801.1"
                     /translation="LNYRIQMTENIDVPVKSLVVIAAALLMLWALQLIVNRTKVGKAI
                     RAVAEDQETASLMGINVNRIISLTFLIGGAMGGAAGVLFGVQYNLINPYTGFIPGIKA
                     FTAAVLGGIGNIAGAMMGGLVLGLLEAFAASYLSLLTGGAFGAEYKDILAFSILIMIL
                     IFRPKGLLGEVVRERA"
BASE COUNT          629 a         1057 c          995 g          514 t
ORIGIN      
        1 ccaccgacag cgcgatggcc gcgttctgct ccaccaggag aatcgtggtt ccctggcgat
       61 tgatctcctc gatggtctgg aagatctgct ccaccaggat cggcgcgagg cccatggaag
      121 gctcgtccaa gaggaggagg cgcgggcggg tcatgatggc ccggccgatg gccagcatct
      181 gctgctcgcc gcccgagagt gtccccgcca cctggagccg gcgctcccgc aggcgcggga
      241 acaggctgaa gacccggtcg atgtcctcgg cgatgccggc gtcggatcga aggtaggcgc
      301 ccatctccag gttttcgacc acggacagcc ggttgaagat cctccgtccc tcgggtgcgt
      361 gggcaatccc caagcccacg atctcgtgcg cgggcagtcc gtggatgggc ctcccctcga
      421 gcaggatccg gccggtccga ggccgcagga cgcccgagat cgtcttgagg ctcgtcgtct
      481 ttcccgcccc gttgttgccc aggagggcga cgatctcccc gtcgcccacg cggacggaca
      541 cacccttcag ggccaggatc tcgccgtaat aaacctggat gtcctcgacg tcaagcatgc
      601 gcgctgtccg ggggcgcccc ccagcgcttc cggcccaggt aggcctcgat cacccgggga
      661 ttcttttgca cctcgctggg ctgcccctcg gcgatcttct ccccgtaatc cagcacggtc
      721 acgcggtccg agacgcccat cacgacgcgc atgttgtgct cgatcagcag gatggtgagc
      781 cggtactcca ggatgagccg ccgcagcagc gccatgaggc tctgcgcctc tccctgggtc
      841 atcccggccg tgggctcgtc caggaggagc agcgtcggtt cggaggccag ggcccgagcc
      901 agctccagtc tccggcgctc gccgtagggg agattcctgg ccacctcgtt ggccttggcc
      961 acaaggccca ccacctccag aagcccggag gcacggctcc acagccggtg ctcctcctgt
     1021 tgaaagtggt gggatcggac gaggacgctg gccatggaga aggcgatccg ggagtgcatc
     1081 cccaccaaga cgttctccag ggcggtcatg ttggagaaga ggcggatgtt ctgaaaggtg
     1141 cggcagatgc ccagcgccgt gatctgatcc ggccgtagcc ccaggagcgg ctttcccttg
     1201 aaatagacct cgcccccctc cggctgatag attcccgtga agacgttgaa gagcgtcgtc
     1261 ttccctgccc cgttgggtcc gatgatggag gcgatgtgcc cctcctccag caggtagtca
     1321 acgtggttga gggccgtgag cccgccgaat ttcttcgtga tctgccgaac atccaggatg
     1381 gcggccacct atttccctcc aaactcgcgg gtgcggcggc gagcagggag aattccttgg
     1441 gggcgaaaga tcatcatgag aatgaggatg gccccgaaga tcatccgctc gtacttggcc
     1501 ggctcgagct gggtcgggag attggccagg ttgtacccga agatgatggt tccggcgttc
     1561 cgcagctcgt tcagccagag cgacaggccc ttgagaacct gcagattcaa caccgtcacc
     1621 accgaggccc ccaggatggc cccggggatg gagcccatcc cgcccaggat caccatggag
     1681 agcaccccga tggattccag gaaggtgaag gactccgggt tgatgaagac ctgcttggcg
     1741 ctgaagaccg cccccatggc acccgcgaag gaggcgccgc aggcaaaggc catcagcttc
     1801 atccggacca gcggaacccc catggcctgc gccgccacct cgtcttcgcg gatggcctcc
     1861 caggcccgtc cgatgtgcga gtcctccagc cggcgattga cctggatgac gatcaggacg
     1921 atgcacagta cgatgaaata gagatagagg gggtagagcg ccaccggcga tgcatggatt
     1981 ccgatgaatt ccaggaccgg ctggaagaac aggggaggcc ggacgatggg cgtgatgcct
     2041 tgggggccgt tggtgaaatt gatgggctta tccaggttat tcgcgaggac ccggatcacc
     2101 tcgccgaagc ccagggtgac gatggccagg taatcgccgc gcaggcggag aaccggaagc
     2161 cccaacagaa tccccgccgt ggcgcccacg caaaccgcca ggatgaggaa cacgaagaac
     2221 cagctccctc cgaggggaaa ggtcgccacc cactccggga tgaatttccc agcctggtgc
     2281 gagccgaaga tggcccaggt gtaggccccc acggcgtaaa aggccacgta cccgaggtcc
     2341 agcagccccg ccaaaccgac cacgatgttc aggcccaagg agagcgccac gaagattccc
     2401 acctgggtgg cgacctccag atagtagccg ttgatcgtcc ccaggatcgg catcacgacc
     2461 cccagggtca ccccgcccag caagagcttc agccagggcc tcacccgcgc gagatagagc
     2521 aacaggatgg atgactggaa gagcaggaag acgagcaccg agcgcgggta gtaggccacc
     2581 ccgaacgacg acagtccgat caccgccatc gtcaggaccg tcgccaacca cggccgttcc
     2641 agtaatgcgc tcaaccaggc cacctacgca cgctccctca cgacttcgcc cagcagcccc
     2701 ttcggacgga agatcaagat catgatcagg atggagaagg cgagaatgtc cttgtattcc
     2761 gccccgaagg cccccccggt cagcaaggaa aggtaggatg cggcgaaggc ttcaagcaac
     2821 cccaacacca acccgcccat catggccccg gcgatattcc cgatcccccc caagaccgca
     2881 gccgtgaacg ccttgatccc cgggatgaac ccggtgtagg ggttgatgag attgtactgg
     2941 acgccgaaga gaactccggc tgctccgccc atggcccccc cgatcaggaa ggtcaaggag
     3001 atgattcggt tcacgttgat tcccatcagg ctggccgtct cctgatcctc cgccacggcc
     3061 cggatggcct tccccacctt cgtccggttc acgatcaact gcagggccca gagcatcagg
     3121 agcgcggccg cgatcaccac cagtgacttc accgggacgt cgatgttctc ggtcatctga
     3181 atacggtagt tgagc
//
LOCUS       DBNGZC010000520         2320 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_44570, whole genome shotgun sequence.
ACCESSION   DBNGZC010000520 DBNGZC010000000
VERSION     DBNGZC010000520.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_44570"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="AC16G0_09085"
     CDS             complement(<1..471)
                     /locus_tag="AC16G0_09085"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5099802.1"
                     /translation="MPETALGFIGLGVMGGPMARILIKAGYPVIGFDTDAGRLAKFTA
                     AGGKAGKSAAEVGKQSEVVFLSLPTGDVVREIVTGANGLATVMKAGTLVADLSTTEPK
                     VAQDAAAACSAKGVVFLDAPVSGGEQGAIDASLSIMVGGDAAAFTRCKPILACLW"
     gene            complement(709..1467)
                     /locus_tag="AC16G0_09090"
     CDS             complement(709..1467)
                     /locus_tag="AC16G0_09090"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5099803.1"
                     /translation="MDFTGKVALVTGAGSGIGRATAGLFAKRGGAVVVADINEASAKE
                     TAETILKNGGRAEACRCDVTVWEQAQTAVGKTRQVFGRLDVLVNCAGILRAHLIEETP
                     ESEWQEVLTTNLTGAFLLTKAAMRVMREQRGGSIVHIASRMALRVKEEHGAYAASKAG
                     VMQLTMMAALEGGPHGVRVNCVCPGFIDTPMTRKSGPKDIVDAQFAGWAKVCPLGRAG
                     MPEDIAKAMLFLASDDAAFITGVALPVDGGRTIL"
     gene            complement(1607..>2320)
                     /locus_tag="AC16G0_09095"
     CDS             complement(1607..>2320)
                     /locus_tag="AC16G0_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5099804.1"
                     /translation="RGGYVLNGTKRFITCAMLADLAVVVATVDRSKGRQGITLFLVEK
                     GTPGFSPGSKEHLLGVRGMGTGEVVFDNCWVSKECMLGGEGEGFKRAMLSLDTGRIGI
                     GCQALGLAQAAMEEAVRYAKQRTAFGQPIANLQAVQFKLADMSAGIEAARLRLRHAAW
                     LKDQGRSMIREAAEAKLLASDLAVKVTAEALQIHGAYGYSTDFPIERMYREAKVYQIW
                     EGTSEIQRVVIARQLLKEY"
BASE COUNT          407 a          845 c          660 g          408 t
ORIGIN      
        1 ccacaggcag gccagaatcg gcttgcaccg agtgaaggcc gcggcatcgc cccccaccat
       61 gatggacaag gaggcatcga tcgccccctg ctcaccgccg ctgacaggcg catccaggaa
      121 gacgacaccc ttggccgagc acgcggccgc cgcgtcctgg gccaccttgg gctcggtggt
      181 gctaaggtcg gcgacgagcg tgccagcctt catgaccgtc gccaggccat tggcacccgt
      241 cacgatctcg cgcacgacat cgccggtcgg cagggagagg aagaccacct cgctttgttt
      301 gccgacctcg gccgccgact ttccagcctt gccgccggcc gccgtgaact tcgcgagacg
      361 cccggcatcc gtgtcgaagc caatgacggg atacccggcc ttgatgagaa tgcgggccat
      421 cggccccccc atgacgccaa gtccgatgaa gccgagtgct gtctcaggca tggacctgct
      481 ccttgaaaga agtgaacctc cggatttcac acccagggat gtaccagtgc ctccgcgaac
      541 gtctggtatt tctggagcgt cagcctaccc gtcctgatga ttgagaggct tccgctaaac
      601 caccacttcc tcccatatct ggccatgatt ctgcagaagc ataccacgag gcagagcgcg
      661 aggacatcgt acggcaggcc atccagcctg cgcacctacg agccgcactc aaagaatcgt
      721 ccgcccaccg tccacgggga gcgcgacccc cgtgatgaag gcggcgtcat ccgaggccag
      781 gaacagcatc gccttcgcga tatcctcggg cataccggcg cggcccagcg gacagacctt
      841 ggcccagccg gcgaattgcg cgtccacgat gtccttgggg ccggacttgc gggtcatggg
      901 cgtatcgatg aagccggggc agacgcagtt gacccggacg ccgtgaggcc ccccctccag
      961 cgcggccatc atcgtgagct gcatgactcc ggccttggat gcggcatacg ccccgtgctc
     1021 ctcctttacc cggagggcca ttctcgaggc gatgtggacg atggagccgc cccgctgctc
     1081 acgcatgacc cgcatggcgg ccttcgtcag tagaaatgcc ccggtcagat ttgtggtcag
     1141 gacttcctgc cactccgact ccggcgtttc ttcaatcagg tgggcccgca ggatgccggc
     1201 gcagttgacc aggacgtcga gtcggccaaa gacctgcctg gtcttcccga ccgccgtctg
     1261 cgcctgctcc cagacggtca catcacagcg gcacgcctcg gcccgaccac cattcttcag
     1321 gatcgtctcg gccgtttcct tggccgacgc ctcattgata tcggccacaa caacggcgcc
     1381 gccgcgcttc gcgaagagcc ccgcagtcgc tcgtccgatc cccgaccctg cgccggtcac
     1441 cagcgctacc ttccccgtga agtccatcgg ctctcccttt ccaaaaggac tgacaacgat
     1501 ctccgacggc gacaggagca cccggctttc ctgcgccgtc cctgtcgcgg tcaccccgta
     1561 gtcgcttccg ccctcactgc acccaatacc tgctactgtc gccctatcag tattccttca
     1621 agagttgtct ggcgatcacc acacgctgaa tctcgctcgt cccctcccag atctggtaca
     1681 ccttggcctc ccggtacatg cgctcgatag gaaagtccgt gctgtacccg taggcgccgt
     1741 ggatctggag ggcttccgcc gtcaccttga ccgctaggtc cgacgccaag agctttgcct
     1801 cggccgcctc acggatcatc gaccggcctt gatccttgag ccatgccgcg tgccgcaagc
     1861 ggagccgtgc cgcctcgatc ccggccgaca tgtcggcgag cttgaactgg accgcctgca
     1921 gattggcgat tggctgcccg aaggccgtgc gctgcttggc gtaccggacg gcctcctcca
     1981 tggcggcctg ggccagaccc agggcctggc agccgatacc gatgcggccg gtgtcgagcg
     2041 acagcatggc ccgtttgaac ccctcgccct cgccgcctaa catacactcc ttggataccc
     2101 agcagttgtc gaaaactact tcgcccgtac ccatgccccg gacacccagc aggtgctcct
     2161 tggagccggg cgagaaaccc ggcgtcccct tctccaccag gaacagggtg atcccctgac
     2221 gccctttgga cctgtccacc gtcgccacca ctacggccaa gtcagccagc atggcgcagg
     2281 tgatgaaccg cttggtgccg ttcaggacat agccgccccg
//
LOCUS       DBNGZC010000521         3029 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2195883, whole genome shotgun sequence.
ACCESSION   DBNGZC010000521 DBNGZC010000000
VERSION     DBNGZC010000521.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2195883"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            300..1229
                     /locus_tag="AC16G0_09100"
     CDS             300..1229
                     /locus_tag="AC16G0_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5099805.1"
                     /translation="MLLTTLFWGGIPVAGKLVIREIPALTAGVLRYGLTSLLLIAACW
                     RQLPDPRALLRSDLWLLLWVGVFGTFLNHILFFMGLVHAPAAHGSIIPPTTSPIWTMI
                     LAARLGKERVTGGQVAGMVLCLVGVVLVIHPERLVTGGGGSLLLGDVLFFLGGGAWGV
                     YSFLSKAAMARLSAITTLTFAMITGTVFLVPWALAERPWMALRAAHLSTWGALAYITL
                     AGTVLSFFWWNLALHRVGAGKTAVFTNLVPVFGVILSWLILGERLTVIQLVGGLLAVG
                     GVLACQRPASPAIARGPVQQRRLRPWTSDKDEA"
     gene            1404..1898
                     /gene="ybaK"
                     /locus_tag="AC16G0_09105"
     CDS             1404..1898
                     /gene="ybaK"
                     /locus_tag="AC16G0_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HIR5099806.1"
                     /translation="MLASALVKTRAAQLLERAGIPYEIREFQEEELGAEEAARKLQIP
                     LAQVFKTLVVRGDRTGVMLACLPGTTTLSLKALAKVSGNRKVEMVEKDEIHRLTGYLR
                     GGVSPLGGKKTYPVHLDQSALTQPRLSVSAGMRGMQIFLTPEALRRATDAKIAALGED
                     QHAS"
     gene            1888..2802
                     /locus_tag="AC16G0_09110"
     CDS             1888..2802
                     /locus_tag="AC16G0_09110"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5099807.1"
                     /translation="MRHEGLVRLRLLLVPLALVTLAGCATTPLRPSPDVVTELVEATT
                     ADGVRLAGALWSPATPDWGRPALLLVHGFAGNFYEAWPSLAERLAARGFPALALNLRD
                     HDLTPQTSRFEDNRLDIAAGIQWLSRRGASRVVLGGQSLGTNRVLFYLAETQDPRVSD
                     LILMAPPGNLFEWNIRIFGRERALAVLAEAEQRVRENRGGDLMLVDLGPLGKALYSAE
                     HILSLRGPHSRSDPFQNIARVRVPILILHGTADRLAEPVVSLRLQAAATAAQSVQVHL
                     IPGADHALPRRPSELLPILEPWLDTVIK"
     gene            2778..>3029
                     /locus_tag="AC16G0_09115"
     CDS             2778..>3029
                     /locus_tag="AC16G0_09115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5099808.1"
                     /translation="MAGHGDQVMQTPEPKDEQDLIAASRAGNASAMEELVRSNQQRVY
                     NFAMRMCRNVEDAKDILQETFLGMVRSIRDFREESKFTTW"
BASE COUNT          471 a         1016 c          974 g          568 t
ORIGIN      
        1 atgtcaagcg ctttcccccg ggaacctggc atctgtcgca caagagatcg atccacctcg
       61 tggccattcg gcgctctgcg gcgggtgggc acgcggtagg cactctcacc tgccctggcg
      121 ctttctcctc atgcgcgggc agttgttgtg atatatagcc ttgttcgtct cgggggagct
      181 cgacacgcac cctcggggta gcaggaagca tcactccatg agccatagcg aggaggagtt
      241 cactgggcac cgtcgggcca cgcgaacgct acgcgccgtt ctcccaaacc tatctgctga
      301 tcctcctgac gacactcttc tggggaggga tcccggttgc cggcaagctt gtcattcggg
      361 agatcccggc cctcacggcc ggcgtgcttc gctacggcct aacctcgctg ctcttgatcg
      421 cagcctgctg gcgtcaactt cccgatccgc gcgcactcct tcgaagcgac ctctggctcc
      481 tgctgtgggt gggggtcttc gggacgttct tgaaccacat cctgttcttc atgggattgg
      541 ttcatgcacc tgccgcccac ggctccatca tcccgccgac caccagcccc atctggacca
      601 tgatcctggc cgcacgcctg ggaaaggagc gggtgacggg gggacaggtg gccgggatgg
      661 tcctctgtct cgttggcgtg gttctggtca ttcatccgga gcgtctcgtg actggaggtg
      721 gaggcagcct cctcctgggg gacgtgctgt tcttcctggg aggcggcgcc tggggggtct
      781 attccttcct ctcgaaggcc gccatggccc ggctgtcggc catcactacc ttgacttttg
      841 cgatgatcac cgggacggtg tttctggtcc cttgggccct ggcggagcgg ccctggatgg
      901 ctcttcgtgc cgcgcacctg agcacctggg gcgcgctggc gtatatcacc ctggcgggca
      961 cggttctgtc tttcttctgg tggaatcttg ccctccaccg ggtcggggcc ggcaagacgg
     1021 cagtcttcac caacctggtg ccggtcttcg gtgtgatcct gtcatggttg atcctggggg
     1081 agcggctgac ggtcattcag ttggtggggg gacttctggc cgttggtgga gttctcgcat
     1141 gccagcgccc cgcatccccc gcgattgccc gcggccccgt gcagcaacgc cggctcaggc
     1201 cctggacctc cgacaaggat gaggcttgac cctgacagcc tcgccggcga ccctcggcca
     1261 ggcagctcaa gaaggtccag atgcgaggcg gcgcccccat tcttcgatgg ggaccagggg
     1321 ctgggcaccc atccaacgca tgggtactcg gctcataggc ctttttcagc agcctgccag
     1381 gacttgacgc tgtctcgccc cgaatgctag cctcagctct cgtgaagact cgagcggccc
     1441 agttgctgga aagagcgggt atcccttacg agatccggga atttcaggag gaggagctcg
     1501 gggcggagga ggctgcccgc aaactccaga tccccctagc ccaggttttc aagacattgg
     1561 tcgtcagggg ggatcgaacg ggagtcatgc tcgcgtgcct gcccgggacg accaccttgt
     1621 ccctcaaggc cctggccaag gtctccggaa accggaaggt cgagatggtg gagaaagacg
     1681 agattcaccg cctcacgggc tacctgcggg gcggggtctc gcccctcgga gggaagaaga
     1741 cgtacccggt gcacctggac caaagcgcgc tgactcagcc caggctgagc gtcagtgcag
     1801 gcatgcgcgg catgcagatc ttcctcaccc cggaggctct ccgacgggcc accgacgcca
     1861 agatcgcggc cctcggagag gaccagcatg cgtcatgagg gtttggtccg cctgcggctg
     1921 ctcctggtgc cgctggcgct ggtgacgttg gccggctgcg cgaccacgcc actgcgccca
     1981 tccccggacg tcgtcacgga gctggtcgag gcgaccacgg ccgacggggt tcggctggcg
     2041 ggcgccctct ggtcccccgc gacaccggat tggggccgac ccgcactcct cctcgtccat
     2101 ggattcgcgg gaaacttcta cgaggcctgg ccgtctctgg cggagcgttt ggcggcccgc
     2161 ggtttccccg ccctggctct caacctgcgc gatcacgacc tcacgcccca gacctcccgt
     2221 ttcgaggaca atcgcctcga catcgcagcg ggaatccagt ggctgtcccg gcggggagcc
     2281 agccgtgtcg tcctcggggg ccagagcctg gggacaaacc gggtgctctt ctacctggcg
     2341 gagactcagg atccgcgcgt gagcgatctc atcctgatgg ctccgcccgg aaacctcttc
     2401 gagtggaaca tccgtatctt tgggcgcgag cgcgccctgg cggtgctggc ggaagcggag
     2461 cagcgggtgc gggaaaaccg gggcggcgac ctgatgctgg tcgatctggg ccccctgggc
     2521 aaggccctct actccgccga gcacatcctc agtctgcgcg gtccccactc ccgctccgat
     2581 cccttccaga acatcgcccg ggtgcgggtg ccgatcctga tcctccacgg aaccgcggac
     2641 cggctggccg agccagtcgt ctccctgcgt ctgcaggccg ccgccacagc ggcacagagc
     2701 gtccaggtcc acctgatacc gggggcggac catgccctcc cccgccgacc cagtgagctc
     2761 ctccccatcc tcgaaccatg gctggacacg gtgatcaagt gatgcagacc cccgagccaa
     2821 aggacgagca ggatttgatc gccgcctccc gcgccggcaa cgcctccgcc atggaggagc
     2881 tggtgcgttc gaatcagcag cgcgtttaca actttgccat gcggatgtgt cgcaacgtcg
     2941 aggacgccaa ggacatcctg caggagacct tcctggggat ggtccgctcc atccgcgatt
     3001 ttcgggagga gagcaagttc acgacgtgg
//
LOCUS       DBNGZC010000522         4536 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1217927, whole genome shotgun sequence.
ACCESSION   DBNGZC010000522 DBNGZC010000000
VERSION     DBNGZC010000522.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1217927"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..403)
                     /locus_tag="AC16G0_09120"
     CDS             complement(50..403)
                     /locus_tag="AC16G0_09120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR5099809.1"
                     /translation="MKLTWKSVVACGALAVLIGFLGAVVVEKAMAQMKGPADFDFAGG
                     AQGKVVFSHEKHLAKDSKCTACHTKLFKMAKGQRSALKMADMNNGQACGACHDGKVAF
                     SVKDAASCTKCHQKG"
     gene            complement(537..1811)
                     /locus_tag="AC16G0_09125"
     CDS             complement(537..1811)
                     /locus_tag="AC16G0_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR5099810.1"
                     /translation="MPWAWRLLAAAVMAAPILLASSVTEAQKLPEDNACIQCHQGLGD
                     PRLSAPPTGFPQDIHLTKGILCQDCHGGDPTSFDPERAMSRAKGFIGKPTHQAIPQLC
                     GKCHSNAVYMKRFNPTIRVDQVQEYFTSVHGRLLQKGDQKVATCISCHDVHRIRAIKD
                     QQAWTYPVKVVDTCGRCHGDPKYMTGYNIPTDVVTKYKQSVHYEALTKKGDLSAPTCN
                     VCHGNHGATPPGVDSVANVCGTCHSVPAEFFGKSPHQKAFAAMGLFPCVTCHSNHDVM
                     KTSDSMVGTTDKAVCLNCHNANTPGFQKAGAIRASLDGLSEDVLRATKILERAENAGM
                     EVSQAKFELRGAADILLKARAAVHLADPVAVKKLTDEGDQIATKMQTRGVKALEELTF
                     RHRGLWIAVGIILVAIVGLVLKIKEIDRPRQG"
     gene            complement(1813..2907)
                     /locus_tag="AC16G0_09130"
     CDS             complement(1813..2907)
                     /locus_tag="AC16G0_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIR5099811.1"
                     /translation="MGEPRLIERLTTWLDERVDLVEIREFIRHKVVPVHSHTVWYYFG
                     GMTLFLFTIQVCTGILLLLYYRPSAEEAYESVQFIVTEVQFGWLIRSIHSWSANLMIA
                     TLMIHMFSVYFTRAYRKPRELTWVSGMLLFGIALTFGFSGYLLPWNTLAYFATKVWTD
                     VAGQVPLVGHLMTRILRGGEDVTGATLSRFYGIHVAVLPLLTTAILGLHLFLVQKQGM
                     SLPVEIEHSGRPAKTMPFFPDFLLRDLVGWLSALAVLGALAAYFPWELGTKADPFAPA
                     PAGIRPEWYFMYMFQTLKYLPAKIGPLDGEMVGVLGFGLGGLFLLLVPFLDRKAAQGE
                     KGPLFLWLGVGIVTYIVILSILGYVAPGGK"
     gene            complement(2910..3353)
                     /locus_tag="AC16G0_09135"
     CDS             complement(2910..3353)
                     /locus_tag="AC16G0_09135"
                     /EC_number="7.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="QcrA and Rieske domain-containing protein"
                     /protein_id="HIR5099812.1"
                     /translation="MDMLANTRRSFVNILLGSSFGAMVVWMLYPVVRYLIPPQSGEPT
                     IASVQMPWKPTELKTNSGRIFKFGSQPGILVKTPAGELRAFSAVCTHLNCTVQYRDDR
                     QDIWCACHNGVYDLNGKNVSGPPPRPLEPYKVFVKGDQIVVTKGA"
     gene            complement(3381..>4536)
                     /gene="hemL"
                     /locus_tag="AC16G0_09140"
     CDS             complement(3381..>4536)
                     /gene="hemL"
                     /locus_tag="AC16G0_09140"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HIR5099813.1"
                     /translation="TDVDGKSYIDYVGSWGPMILGHAHPQVVAAIQKTAERGTSYGAP
                     TELETRLAQLVQAAFPSLERMRFVSSGTEACMSALRVARGFTRRDAILKFEGCYHGHA
                     DSLLVKAGSGAITFGIPDSLGVPAELAKHTLTLPYNDLEAVRALFSARGAEIAAVIVE
                     PVAGNMGVVPPQSNFLAGLREITRRHGTLLIFDEVITGFRIARGGAQETTGIRPDLCC
                     LGKIVGGGLPVGVYGGRAEILEQVAPLGPIYQAGTLSGNPLAMAAGIETLTILQTPGF
                     YADLEAKGKRLAEALAEAAAASRIPVQINRVGSMLTAFFTTVPVRDYVTAKTADTARY
                     GAFFRGLLERGVSLAPSQFEAAFISGAHTTQDLEMTIAGAREVLAVLTAA"
BASE COUNT          787 a         1472 c         1435 g          842 t
ORIGIN      
        1 ccccggatgg gcttccacat tcaggcgagg cgagaagtcg aggatgttat cagcccttct
       61 gatggcattt ggtgcagctg gctgcatcct tcacgctgaa ggcgaccttg ccgtcatggc
      121 aggccccaca cgcctggccg ttgttcatgt ccgccatctt cagggcggac cgctgtccct
      181 tggccatctt gaagagtttc gtgtggcagg cggtgcactt cgaatccttg gccaagtgct
      241 tctcgtgact gaacaccacc ttgccctgag ccccgccagc gaaatcaaaa tccgcgggtc
      301 ccttcatctg ggccatggcc ttctccacca ccaccgcgcc caggaacccg atgagaactg
      361 ccagggcgcc acaggccact acggacttcc acgtgagctt cattcaattc acctcctttc
      421 attacaggta ttcttacgtg tcggtgcaat ggctatcgac ccttggacgg ccgaccctgc
      481 tcacctattc actttaggcg gcacccgggc agggttgttc ccacccacgg ctttccctac
      541 ccttgccgcg gccggtcgat ttccttgatc ttcaggacca ggcccacgat ggccaccagg
      601 atgataccca cggcgatcca caggcccctg tgtcggaagg tgagctcctc cagtgccttc
      661 acccctcggg tctgcatctt ggtcgcgatc tggtcccctt catccgtcaa ctttttcaca
      721 gcaaccggat cggccagatg gactgccgcc cgcgccttga gcaggatatc cgcggcgccc
      781 cggagttcga acttggcctg gctcacctcc atcccggcat tctccgcccg ctccaggatc
      841 ttcgtggcgc gcagcacgtc ctcgctcaat ccatccagtg aggcccggat cgcccccgcc
      901 ttctggaatc ccggcgtgtt ggcattgtgg caattgaggc agaccgcctt gtcggtggtc
      961 cccaccatgc tgtcgctcgt cttcatcacg tcgtgattgc tgtggcaggt cacgcagggg
     1021 aacagaccca tggcggcgaa agccttttgg tgtggactct tcccgaagaa ttccgcgggg
     1081 acactgtgac aggtcccgca cacgttggcc accgagtcga ccccgggcgg cgtggcgccg
     1141 tggttgccat ggcagacgtt acacgtgggc gccgagaggt ctcctttctt ggtcagggct
     1201 tcgtagtgaa cgctttgctt gtacttcgtc accacatccg tgggaatgtt gtagcccgtc
     1261 atgtacttgg gatcgccgtg gcaccgcccg caggtgtcca ccactttcac cggatacgtc
     1321 catgcctgct ggtctttgat cgcccggatc ctgtggacgt cgtgacagct gatgcaagtg
     1381 gccaccttct ggtcgccctt ctggagcagc cttccatgga cgctggtgaa gtactcctgc
     1441 acttggtcca cgcgaatggt tggattgaat ctcttcatgt agacagcatt gctgtggcac
     1501 ttcccgcaga gctggggaat cgcctggtgg gtcggcttgc cgatgaaccc cttggcccgg
     1561 ctcatggctc gttctggatc gaagctggtt ggatcaccgc cgtggcagtc ctggcagaga
     1621 atccccttgg tcagatggat gtcttgggga aagcccgtgg gaggcgctga gagacgggga
     1681 tcaccgagcc cctggtggca ctgaatgcac gcgttatcct caggcagctt ctgggcctcc
     1741 gtcacgctcg aagcgaggag gatcggggca gccatcacgg ccgccgccag caggcgccac
     1801 gcccagggca tgtcacttcc ctcccggagc cacgtagccc agtatgctca ggatcacgat
     1861 gtaggtcacg atccccaccc cgagccaaag gaacagcggg cccttctcgc cctgggccgc
     1921 cttccggtcc aaaaatggaa ccaggagcag gaacagaccg cccaggccga aacccaggac
     1981 gccgaccatt tcgccgtcca gcgggccgat cttggcgggc aagtacttca gggtctggaa
     2041 catgtacatg aagtaccact cgggacggat gcccgcgggg gctggggcga agggatcggc
     2101 cttggtgccc agctcccacg gaaagtaggc cgccaacgcc ccgaggactg ccagggccga
     2161 tagccagccc accagatccc ggaggaggaa gtcgggaaaa aagggcatgg tcttggcagg
     2221 gcgcccgctg tgctcgatct ccacgggaag actcatcccc tgcttttgca ccaggaagag
     2281 gtggagaccc aggatggccg tcgtgagcaa tggcaggacc gccacgtgga tcccgtagaa
     2341 tcgggagagg gtggcccccg tgacatcctc gcctccccgg agaatccgcg tcatcaggtg
     2401 acccaccagc gggacctggc cggccacgtc ggtccacacc ttggtggcga agtaggccag
     2461 ggtgttccag ggcaggaggt acccgctgaa cccgaatgtg agcgcgatcc cgaagagcag
     2521 catgccgctc acccaggtca gctcccgtgg cttccggtag gcccgggtga agtacacgct
     2581 gaacatgtgg atcatcagcg tggcgatcat caggttggcc gaccagctgt ggatggagcg
     2641 gatgagccag ccgaactgga cctcggtgac gatgaactgg acgctctcgt acgcctcctc
     2701 ggcgctgggg cgatagtaca gcagaagcag aatcccggtg cagacctgga tggtgaagag
     2761 gaagagcgtc atcccgccga agtagtacca gaccgtatgg ctgtggaccg gcacgacctt
     2821 gtgccggatg aactcgcgga tctccaccag gtcgacccgc tcatccagcc aggtggtcag
     2881 gcgctcgatg aggcgtggct cccccatgct caggccccct tggtcacgac gatctggtcc
     2941 cccttgacga agaccttata gggctccagg gggcggggcg gcgggccgct cacattcttg
     3001 ccgttcaggt catacacgcc gttgtggcag gcacaccaga tgtcttgccg atcgtcccgg
     3061 tattgcaccg tgcagttcag gtgggtgcag accgcgctga aggcgcgcaa ttcacccgct
     3121 ggggtcttga ccaagattcc gggctggctg ccgaacttga agatccgtcc cgagttcgtc
     3181 ttcagctcgg tcggcttcca cggcatttgc accgaggcga tggtgggctc gccgctctgg
     3241 ggagggatga ggtagcgaac gacggggtag agcatccaca cgaccatggc cccgaagctc
     3301 gaacccaaga gaatattgac gaagcttcgc cgggtgttcg ccagcatgtc catgcgctct
     3361 cccctggcat tgaccgcatg ctatgcggcc gtgaggacgg cgagcacctc tcgggcgccc
     3421 gcgatggtca tctccagatc ctgcgtcgtg tgcgccccag agatgaaggc ggcctcgaac
     3481 tgggacgggg ccaggctcac cccacgctca agcaatcctc ggaagaatgc gccataacgc
     3541 gccgtgtccg ccgtcttggc cgtgacgtag tccctgaccg gcaccgtcgt gaagaaggcc
     3601 gtcagcatcg accccacgcg gttgatttgc accgggatcc ggctcgcggc ggccgcctcg
     3661 gccagcgcct cggccaggcg cttccccttg gcctccagat cggcgtagaa gccgggggtc
     3721 tggaggatcg tcagggtctc gatgccggcc gccatggcca gggggtttcc cgacagggtg
     3781 cccgcctggt agatgggtcc cagtggggca acctgctcca ggatctcggc gcggccgccg
     3841 tacaccccga ccgggaggcc gccgcccacg atcttcccca gacagcagag gtccggtcgg
     3901 atgcccgtgg tctcctgcgc cccgccgcgc gcgatgcgaa agcccgtgat aacctcgtcg
     3961 aagatgagga gggtgccgtg gcggcgcgtg atctcgcgca ggcccgccag gaagttggat
     4021 tggggaggga cgacgcccat attgcccgcc acgggttcca cgatgaccgc ggcgatctcc
     4081 gccccgcgcg ccgagaaaag tgcccgtacg gcttccaggt cgttgtaggg gagggtgagg
     4141 gtgtgcttgg ccagttcggc ggggacgccc aggctgtccg gaatcccgaa ggtgatcgct
     4201 ccggatccgg ccttcaccag caggctgtcg gcgtgcccgt ggtaacagcc ctcgaacttg
     4261 aggatggcgt cacgccgggt gaagccgcgc gccacccgga gggcgctcat gcaggcctcg
     4321 gtcccggagg agacaaagcg catccgctcc aggctgggga aggccgcctg gacaagctgg
     4381 gccagacgcg tttccaactc cgtgggggcc ccgtagctcg tcccccgctc cgccgtcttt
     4441 tgaatggcgg cgacgacctg tgggtgggcg tggcccagaa tcatgggtcc ccaggagccg
     4501 acgtagtcga tgtagctctt gccgtccacg tcggtg
//
LOCUS       DBNGZC010000523         1295 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2392520, whole genome shotgun sequence.
ACCESSION   DBNGZC010000523 DBNGZC010000000
VERSION     DBNGZC010000523.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2392520"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..>1295
                     /locus_tag="AC16G0_09145"
     CDS             122..>1295
                     /locus_tag="AC16G0_09145"
                     /inference="COORDINATES: protein motif:HMM:NF014073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR5099814.1"
                     /translation="MEIFHNILLGFQVAMSWQNLLFCLIGVTYGTLIGVLPGIGPVVG
                     VAILIPVTFGLSATTAIITMAGVYYGAMYGGSTTSILINVPGESASVVTCLDGYQMAK
                     KGRAGPALGMAAMASFMAGTLAVVLLTFIAPALANVALSFGPPEYFALTFMGLTLVTS
                     LAGESLVKGLMSGVFGLIVACVGVDAVSGEERFTFGILSLLDGMGFIGVAVGLFAVAE
                     VLCNLEESMEQIFVKAKLGLRNLFPNWQDWKDSFLPIWRSSFMAYGMEKKLSKHPEKF
                     GTGIIEGVAAPEAANNAAAGGAMVPLLALGIPSSGTSAVLLGALLVHGLRPGPLLFQS
                     NPDFVWGVIASMYIGNIMLLILNLPPVGLWASLLRIRYKILMPLIVTISAVGVYTTD"
BASE COUNT          194 a          429 c          411 g          261 t
ORIGIN      
        1 ggcgtcagtc ttccaaagaa cctgttgggt ttctgacggc aagggcgggg tctgccgagg
       61 gcttcttctc ggagggcggg cggcggtgac gccgcggccc gccgggctag ggaaggcacc
      121 catggagatc ttccacaaca tcctgctggg ttttcaggtg gcgatgtcct ggcagaacct
      181 cctcttctgt ctaatcggcg tcacctacgg cacgctgatc ggcgtcctgc cagggatcgg
      241 ccccgtggtc ggtgtggcga tcctcatccc ggtcaccttt ggcctcagcg ccaccacggc
      301 catcatcacc atggccggcg tctactacgg ggcgatgtac ggcggctcaa ccacctcgat
      361 tctcatcaac gtccccggcg agtctgcctc ggtcgtcacc tgcctggacg gctaccagat
      421 ggccaagaag gggcgggccg gcccagccct gggcatggcc gccatggcct ccttcatggc
      481 cgggaccctg gcggtcgtcc tgctcacctt catcgcgccc gcgctggcaa acgtcgccct
      541 ttccttcggg ccgccggagt atttcgccct gaccttcatg ggtctcaccc tggtgaccag
      601 cctcgccgga gagtcattgg tcaagggcct catgagcggc gttttcggct tgatcgtcgc
      661 ctgcgtgggc gtggacgctg tcagcgggga ggagcgattc acgttcggca tcctctccct
      721 cctcgacggc atgggcttca tcggggtggc agtgggcctc ttcgcggtag ccgaggtgct
      781 ctgcaacctg gaagagtcca tggagcagat cttcgtcaag gcgaagctgg gcctccggaa
      841 cctcttcccg aactggcagg attggaagga ttccttcctc cccatctggc gctcgtcctt
      901 catggcctac ggcatggaga agaagctgag caaacacccg gagaagttcg gcacgggaat
      961 catcgagggg gttgccgctc ccgaagcggc caacaatgcg gcggccggtg gggcgatggt
     1021 tcccctcctg gcactgggca tcccctcctc cggcaccagc gccgtgcttc tgggggcgct
     1081 cctggtccac gggctgcggc cggggccgct tctgttccag agcaacccgg atttcgtctg
     1141 gggcgtgatc gccagcatgt atatcggcaa catcatgctg ctgatcctga acctgccccc
     1201 ggtcgggctc tgggccagcc tgctgcgcat tcggtacaag attctcatgc cgctgatcgt
     1261 gaccatatcc gccgtcgggg tctacaccac ggaca
//
LOCUS       DBNGZC010000524         2263 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_727792, whole genome shotgun sequence.
ACCESSION   DBNGZC010000524 DBNGZC010000000
VERSION     DBNGZC010000524.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_727792"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1360)
                     /locus_tag="AC16G0_09150"
     CDS             complement(<1..1360)
                     /locus_tag="AC16G0_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5099815.1"
                     /translation="MAGRLARLFIDSRLTPLVVLASMLLGFFAIIATPREEEPQIIVP
                     MMDVFVQMPGAGAKEVEERVTIPMEKKLMEIPGVEYVYSTSSPGMSQAIVRFKVGEDE
                     EKSIVKLYNKMYANFDLIPPGASTPLIKPRSIDDVPILALTLWSDRVDGYTLRRIAAQ
                     LDDQIKDIQDVSETRLLGGERRQVRIQLDPTALAAHQLAPAMVVGALTAANRELRAGS
                     FTRDNREILVDTARWLERPEEVGRVVVGAPDGRPVYLKDVARILDGPEEPRDYVFFGV
                     GRAAHAKGIRGERPAGQEYPAVTISVAKRKGTNAVVIADKVLAKVASLQGVLIPREVQ
                     VTVTRNYGETAQDKSNELLKHLTLATLSVTILIALFLGWRSAVVVLMAVPVTLALTLF
                     LYYIYGYTLNRVTLFALIFSIGILVDDAIVVVENIARHFHLPENRGRPLLAVAVEAVA
                     EVG"
     gene            complement(1469..>2263)
                     /locus_tag="AC16G0_09155"
     CDS             complement(1469..>2263)
                     /locus_tag="AC16G0_09155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5099816.1"
                     /translation="EAQRDLAASTLARYQQLLDRKAVAPLEYDQVLARQKAAFADVER
                     ALAEEQAVQAKRQQVLAKIEGAKAEIASLQVTQGYAKIAAPFAGVVTQKHADVGSLAA
                     PGTPILTLEDGRRYWLEASVPGSQISGIRRGQSLRVQVEEAGLSITAGVSEILPSADP
                     TTRTTLVRLDLPASPRLRSGFFGRVWVPVGRRQSIQVARGAIVERGQLQGVYVVGQDN
                     IARFRLVRTGQTSRDATEILSGLTGGEQVVLGGTERVTDGARIERK"
BASE COUNT          367 a          752 c          769 g          375 t
ORIGIN      
        1 tccccacctc ggcgaccgcc tcgacagcca cggcgagcag gggtcgcccc cggttctcgg
       61 gcaagtggaa gtggcgcgcg atgttctcca ccaccacgat ggcatcgtcc accagaatgc
      121 cgatcgagaa gatcagcgcg aagagggtta cccggttcag cgtgtagccg tagatgtagt
      181 acaggaacag ggtcagggcc aacgtcaccg gcaccgccat gaggaccacg accgccgagc
      241 gccagcccag gaagagggcg atgaggatgg tcaccgagag cgtggccagg gtcaggtgct
      301 tcagcagctc gttggatttg tcctgagccg tctccccgta gttgcgcgtg accgtgacct
      361 gcacctcgcg ggggatgagg actccctgga gcgaagcgac cttcgccagg accttgtccg
      421 cgatgacaac ggcgttcgtc cccttgcgtt tcgccacgga gatcgtgacc gcggggtact
      481 cctggccggc cggccgctcc ccacgaatgc ccttggcgtg cgcggcgcgc cccacgccga
      541 agaagacgta gtcgcgcggc tcctcgggcc catccaggat gcgggccaca tccttcaggt
      601 acacggggcg accgtccggg gcgcccacga ccacgcggcc cacctcctcg ggccgctcga
      661 gccaacgagc cgtgtccacc aggatctcgc ggttgtcgcg ggtgaagctg ccggcgcgta
      721 gctcccggtt tgccgcggtc agcgccccga ccaccatggc gggggccagt tgatgggcgg
      781 ccagggccgt aggatcgagc tggatgcgca cctgccgtcg ctcgcctccc aggagacggg
      841 tctcggacac gtcctggatg tccttgatct ggtcgtcgag ctgggcagcg atccggcgta
      901 gggtgtagcc atccacccgg tcgctccaga gggtgagggc caggatcggc acatcgtcga
      961 tggagcgcgg cttgatcagc ggggtggagg cgccgggtgg gatgaggtcg aagttggcgt
     1021 acatcttgtt gtacagcttg acgatgctct tctcctcgtc ctcgcccacc ttgaagcgga
     1081 caatggcttg gctcatcccc gggctgctcg tggagtagac atactctacg cccgggatct
     1141 ccataagctt cttctccatg gggatggtga cgcgctcctc gacctccttg gcgccggccc
     1201 cgggcatctg aacgaagaca tccatcatgg ggacgatgat ctggggctcc tcctcgcggg
     1261 gggtggcgat gatggcgaag aagcccagca acatggaggc gagcaccacc aggggggtca
     1321 ggcgcgagtc gatgaagagg cgcgctagcc tacccgccat gcccagggaa ggatggtgat
     1381 cggaagcgcg cgatgcgggc tgagcgtctg tcatggggat ccgtcacaat acacacatga
     1441 aagcataaga gcgcgtgcgc ttcccgttct atttgcgttc gatgcgcgcg ccgtcggtca
     1501 cgcgctccgt gccgccgagc accacctgct cgccgccggt caacccggac aggatctccg
     1561 tggcgtctcg gctggtctgg cctgtccgca cgagtcggaa gcgggcgatg ttgtcctggc
     1621 ccaccacgta gaccccttgg agctgccctc gttccacgat tgccccgcga gcgacctgga
     1681 tggactgccg ccgacccacg ggcacccaga cccggccgaa gaagccggac cggaggcgag
     1741 gtgaggcggg gagatccagt cgcaccaggg tggtccgggt ggtggggtcg gcggaaggaa
     1801 ggatctcgct cacgccggcc gtgatggaaa gccccgcctc ctcgacctgc acccgcagcg
     1861 actgcccacg gcggataccg ctgatctgcg atcccgggac cgaggcctcc agccagtacc
     1921 gccggccgtc ctcgagggtg aggatcggcg tccccggagc cgccaaggat cccacatcgg
     1981 cgtgcttctg cgtgacgacg ccggcgaagg gggcggcgat cttggcgtaa ccctgagtga
     2041 cctggaggct cgcaatctcg gccttggccc cctcgatctt ggccaggacc tgctgtcgct
     2101 tggcctgcac cgcctgctct tcggccaggg cccgctccac gtcggcaaac gcggccttct
     2161 gtctcgccag aacttggtcg tactccaggg gggccacggc cttccgatcc agtaactgct
     2221 ggtaccgggc cagggtcgat gcggcgaggt cgcgttgcgc ctc
//
LOCUS       DBNGZC010000525         1110 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_436171, whole genome shotgun sequence.
ACCESSION   DBNGZC010000525 DBNGZC010000000
VERSION     DBNGZC010000525.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1110)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_436171"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="AC16G0_09160"
     CDS             <1..832
                     /locus_tag="AC16G0_09160"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5099817.1"
                     /translation="KGLFKAAGLDPEKAPVTWEQVAAYSKQIKEKTGKFGYGMVARLN
                     HGNTPFRIVPVMWGYGGSIFDELDPAPKNKEIRINGEGTVKALETYQRMYAVDKSVSP
                     TALTDAQPEVSTQFLNGQVAMIIAHANDASDAKRKNPDLNVGAGLMPRGPVRRAAVLG
                     GFQLALLKTSKNPVAAMALMKMFMDPEWLPQILGDNSNPATKEALASKAQAERERRNP
                     FHKPYKEMLAYGVVQPQVPQLAPIWNETIPTMLQETITGKKSAKQAADDAAKAISTIQ
                     "
     gene            914..>1110
                     /locus_tag="AC16G0_09165"
     CDS             914..>1110
                     /locus_tag="AC16G0_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099818.1"
                     /translation="MNGMQREGIAFPGRRGEAWTLIWANRRDYPYVLPAILAISLVML
                     YPLGYVIYLSFFRTSLFSPIP"
BASE COUNT          235 a          347 c          355 g          173 t
ORIGIN      
        1 caaggggctc ttcaaggcgg ccgggctgga tccggaaaag gctccggtca cctgggagca
       61 ggtggcggcc tactcgaagc agatcaagga gaagaccggg aagttcggct acggcatggt
      121 cgcccgcctc aaccacggga acacgccgtt ccgcatcgtg ccggtgatgt gggggtacgg
      181 cggcagcatc ttcgacgagc ttgatcccgc ccccaagaac aaggagattc gaatcaacgg
      241 cgagggtacg gtcaaggcac tggagacgta ccagcgcatg tacgccgtgg acaagagcgt
      301 ctcccccacg gcactcacgg acgcccagcc cgaggtgagc acccaattcc tgaatgggca
      361 ggtggcgatg atcatcgccc acgccaacga cgcgagcgac gccaagcgca agaacccgga
      421 cctgaacgtt ggcgccggcc tgatgccacg cggcccggtc cggcgagctg ccgtgctggg
      481 tggctttcag ttggctctgc tcaaaaccag caagaacccc gtcgcggcga tggcgctcat
      541 gaagatgttt atggatcccg agtggcttcc acagatcctc ggtgacaaca gcaacccggc
      601 gacaaaggag gcgctggcga gcaaggccca ggccgagcgg gagcggcgga atccgttcca
      661 caagccctac aaagagatgc tcgcctatgg cgtggtgcag ccccaggtgc cccagctggc
      721 ccccatctgg aacgaaacga tcccgaccat gctgcaggag accatcacgg ggaagaagag
      781 cgcaaagcag gctgccgatg atgccgccaa ggcgatcagc acgatacaat agtgaaggtc
      841 cgggtcagcc tggtgagact gactcaatag cggggaggtg ggaggccggt gtgtgcgccg
      901 ccctcccgcc gaaatgaacg gtatgcaacg ggaggggatc gcgtttcctg gccgccgtgg
      961 cgaggcttgg accctgatct gggcgaaccg ccgggactac ccctacgtcc tgccagcgat
     1021 cctcgccatc tccctcgtga tgctctatcc gcttggatat gtcatctacc tgagcttctt
     1081 cagaacgtcc ctgttcagtc ccattccgag
//
LOCUS       DBNGZC010000526         1860 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_142678, whole genome shotgun sequence.
ACCESSION   DBNGZC010000526 DBNGZC010000000
VERSION     DBNGZC010000526.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_142678"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="AC16G0_09170"
     CDS             complement(<1..579)
                     /locus_tag="AC16G0_09170"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099819.1"
                     /translation="MSHVWLSTLSRLATILILLVVLSLVSPAFLSVQNLVNIIRQASL
                     LFLLASGLTIVILTAGIDLSIGSVLAFSASVAANFIQQGWIWAGSIVGLVIGAGCGLL
                     NGLMVAVVGLPPFIATYGMLWIAAGFAQVFMKGEIISGFPSGFRFLGAGHLTGIPVPI
                     LVMIVVLAVCLFTLRRTNLGRYVYIIGANREAA"
     gene            complement(581..1528)
                     /locus_tag="AC16G0_09175"
     CDS             complement(581..1528)
                     /locus_tag="AC16G0_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099820.1"
                     /translation="MSQTVRYEWTRRLAMAPGAVYALILLSLIFTLFTAQFLTVHNLT
                     NVGMQGSVLIIVSLGMTLIILSEGIDLSLGPVLGLAGVVLALCVKQQVGLPLAILLAI
                     LVGAVFGALNGVLISHVGLPPFVVTLGTFGMAQGLALVLTEGSSVPGLGPAVQYFNDG
                     RILGVQVPIILTGIAFLVVYVILYHTKFGTYVFAIGGNQEALTLAGVRTRLYKTGVYV
                     LGGSLAGVGALVMTARMNAAHPTVGVGLEFDAIASVILGGTSFERGKGWLGGTIIGAL
                     AVSVLRNGLNLIGLSTSWQVSMVGLVIILAIVVDSLRGI"
     gene            complement(1525..>1860)
                     /locus_tag="AC16G0_09180"
     CDS             complement(1525..>1860)
                     /locus_tag="AC16G0_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5099821.1"
                     /translation="TPSINRMTKFLSGGTQQKVVLAKWLCTQSRFLIFDEPTRGIDVG
                     AKAEIHQLMNELVKQGAAILMISSELPEILNMSDRIYVMHKGRISKEVSREEATQEAI
                     LRHAMGVTK"
BASE COUNT          397 a          597 c          576 g          290 t
ORIGIN      
        1 ggccgcctcc cggttcgctc cgataatgta cacgtaacgg cccaggttcg tccgacgcag
       61 ggtgaaaagg cagacggcaa gaaccacgat catcaccagg atcgggaccg gaatcccggt
      121 cagatgccct gcccccagga accggaagcc ggaaggaaac cccgagatga tctcgccctt
      181 catgaacacc tgggcaaagc ccgccgcaat ccacagcatg ccgtaggtgg cgatgaaggg
      241 gggaaggccc accacggcca ccatgagccc gttcagcagt ccgcagcccg ccccgatcac
      301 caggcccacg atgcttccgg cccagatcca cccctgctgg atgaagttgg cggcgacgct
      361 ggccgagaag gccaggaccg atccaatcga gagatcaatg cccgccgtca agatgacgat
      421 cgtcaacccc gaggccagca agaataggag agaggcctgc cggatgatat tgaccaggtt
      481 ctggaccgag aggaaggctg ggctcacgag ggagagcacc accagcagaa tcaggatcgt
      541 agccagccga gacagcgtgc tcaaccagac gtgtgacatg tcagatccct cgcagggagt
      601 ccaccacgat ggccaggatg atcaccagcc ccaccatgga cacctgccaa gaggtgctca
      661 agccgatgag attcagcccg ttgcgcagca cgctgacggc cagggccccg atgatcgtgc
      721 cgccgagcca tcctttgccg cgctcgaagg aggtgccgcc caggatcacc gaagcgatgg
      781 cgtcgaactc cagcccgact ccgaccgtgg gatgggccgc gttcatccgg gccgtcatga
      841 ccagggcgcc cacgccggcc aagctgcccc ccaggacgta cactcccgtc ttgtagagac
      901 gtgtccggac cccggcgagg gtcagggcct cctggttccc cccgatggcg aagacatacg
      961 ttccgaactt ggtgtggtac aggatgacgt agactaccag gaaagcgatc cctgtcagga
     1021 tgatggggac ctggactccg agaatccgcc cgtcgttgaa gtactggact gccgggccca
     1081 gcccggggac cgatgaccct tcggtcagca ccagggcgag accttgggcc atgccgaagg
     1141 tgcccaacgt caccacaaac gggggaagcc cgacgtggga aatcaggacg ccgttcagag
     1201 ccccgaagac ggcgcccacc aggatggcga ggagaattgc cagcgggagt cctacctgtt
     1261 gcttcacgca cagggccagg accacgccgg caagacccag caccgggccc agggacaggt
     1321 cgatcccctc cgaaaggatg atgagggtca tcccgaggga cacgatgatc aggaccgatc
     1381 cctgcattcc gacgttggtc agattgtgta ccgtcaggaa ctgggcggtg aacagggtga
     1441 agatcaggct gagcaagatg agcgcgtaca ccgcccccgg tgccatggcc aggcgccggg
     1501 tccactcgta ccgcacagtc tggctcattt cgtgactccc atggcatgcc ggaggatggc
     1561 ctcttgggtc gcctcctccc ggctcacctc tttgctgatc cgtcccttgt gcatgacata
     1621 gatcctgtcg ctcatgttca ggatttcggg aagctcggac gagatcatca ggatggccgc
     1681 cccttgcttc accagctcgt tcatgagctg gtggatctcg gccttggcgc cgacgtcgat
     1741 cccccgagtg ggctcgtcaa agatcaggaa gcgggactgg gtgcagagcc acttcgctag
     1801 gacgaccttc tgctgggtcc caccactcag gaacttggtc atccgattga tggagggggt
//
LOCUS       DBNGZC010000527         2240 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2000398, whole genome shotgun sequence.
ACCESSION   DBNGZC010000527 DBNGZC010000000
VERSION     DBNGZC010000527.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2240)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2000398"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="AC16G0_09185"
     CDS             complement(<1..875)
                     /locus_tag="AC16G0_09185"
                     /inference="COORDINATES: protein motif:HMM:NF024772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099822.1"
                     /translation="MEQRWRWGVNLLVVGLMVVVSVASASAQGPRVKVREAHTDTLFM
                     APVYIAEAAGYMAEEGIDLEMTEVDSGALGIAALVSGGVQLVDADPFQAVQLLNQGKS
                     IQFMYNLTKGVTLDMVMSSEVVKAKNLSRSMPIAQRFAALKGLKLGITKPGAATDVYM
                     RYYLKRAGIDPERDVQIVTVGGGAALLATMRTRQIDAFHLSAPTPYLAQRDGFGEVII
                     KASAGDVPELTNFLYTGIAVNKEYGDKNVDVLKRWTKAVNRANVLMRKDEGAAITHLR
                     KYFPRMDPAVLTLAMR"
     gene            complement(904..1788)
                     /locus_tag="AC16G0_09190"
     CDS             complement(904..1788)
                     /locus_tag="AC16G0_09190"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099823.1"
                     /translation="MTTGTRENQSTGVAAAGGRGQQLLREAEARDREIEGKNKRNTLL
                     GQALLGIALFCLWEFGSGVIVDQFFVSRPSRIALSLWAMFTKEDLLYHMQFTVVEALV
                     GYTIGASAGLTAGFALARIDLIYRIVQPFIIAFYGVPRIALAPLFILWFGIGISSKLA
                     VAAVMVFFIVLINTIAGIRSVPVQLLQVTRVMGASEWDLVWKVVFPAATPFIIAALQI
                     AVPQAMIGAIVGEFISSNRGVGHLINRASGWLDTPGLFAGILALMVVVLLMNYGVTLL
                     GNRLMRWNPKYSSMREPV"
     gene            complement(1785..>2240)
                     /locus_tag="AC16G0_09195"
     CDS             complement(1785..>2240)
                     /locus_tag="AC16G0_09195"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020663342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099824.1"
                     /translation="RHEQAATYIAKVGLSGFENHYPHELSGGMRKRAALVRTLIYEPD
                     IILMDEPFGPLDAQTRVILQDELLKLWDGSGKTIIFVTHDLVEAIALSDRIVLFSRAP
                     GTIKHVYEVPMDRPRDVFHIHAAPAFPEFFDRLWRDLKEEITEVELRAK"
BASE COUNT          410 a          743 c          690 g          397 t
ORIGIN      
        1 cgcatcgcca gggtcaggac ggcgggatcc atgcgcggga agtatttccg aagatgggtg
       61 atggccgcgc cctcgtcctt gcgcatcagg acattggccc ggttcaccgc cttggtccac
      121 cgcttgagga cgtcgacgtt cttgtcgccg tattccttgt tgacggcgat gcccgtgtag
      181 aggaagttgg ttagctccgg cacgtcgccc gccgacgcct tgatgatcac ctcgccgaag
      241 ccgtcccgtt gcgccaggta cggcgtgggg gcggagaggt ggaaggcgtc gatctggcga
      301 gtgcgcatgg tcgccagcaa ggccgccccg cccccgaccg tcacgatctg cacgtcccgc
      361 tccgggtcga ttccggcacg cttgaggtag tagcgcatgt agacgtcggt cgcggcgccc
      421 ggcttggtga tccccagctt gaggcccttc agcgccgcga accgctgggc gatcggcatg
      481 ctccggctga ggttcttcgc cttgaccacc tcggaggaca tcaccatgtc cagggtcacg
      541 cccttggtga ggttatacat gaactgtatc gacttgccct ggttcaagag ctgcacggcc
      601 tggaagggat cggcatccac cagctgcacg cccccggaaa cgagggccgc gatgcccagg
      661 gcgccgctgt ccacctcggt catctcaaga tcgatcccct cttccgccat gtagccggcc
      721 gcctccgcga tatacaccgg ggccatgaag agcgtatcgg tgtgggcctc gcggaccttg
      781 acccgcggcc cctgcgcgga cgcgctggcc actgaaacca caaccatcag cccaacgacc
      841 aacaggttca ccccccaccg ccacctctgt tccatgttgc cccccttgtc gcgttacggg
      901 tttctatacc ggctctcgca tgctgctata cttcgggttc cagcgcatca aacggttgcc
      961 gagcaacgtg accccgtagt tcatcagcag caccaccacc atgagggcca ggatccccgc
     1021 gaagaggccg ggagtgtcga gccagccgga cgcccgattg atcaggtgcc caacgccccg
     1081 gttgctggag atgaactcgc cgacgatggc gccgatcatc gcctggggca ccgcgatctg
     1141 cagcgcggcg atgatgaacg gggtcgcggc gggaaagacg accttccaga caagatccca
     1201 ttccgaagcg cccatcaccc gtgtcacctg caggagctgg accgggacgg atcggatgcc
     1261 ggcgatggtg ttgatcagca cgatgaagaa gaccatgacc gccgccaccg ccagcttgga
     1321 gctgatgccg atcccgaacc agagaatgaa cagtggcgcc agggcgatgc gcgggacgcc
     1381 gtagaacgcg atgatgaacg gttggacgat gcggtagatt agatcaatgc gggcgagggc
     1441 gaaccccgcc gtcagcccgg cgctggcgcc gatggtgtag cccaccagcg cctcgaccac
     1501 cgtgaactgc atgtggtaca gcaggtcctc cttggtgaac atggcccaca gggatagcgc
     1561 gatccgactg ggacggctga cgaagaactg atccacgatg acaccggagc cgaattccca
     1621 gagacagaac agggcaattc ccaggagcgc ctgtcccagg agcgtgttcc gcttgttctt
     1681 cccctcgatc tcccgatctc gtgcctcggc ctcgcgcaaa agctgctggc cccggccccc
     1741 ggcggccgcg acccccgtcg actgattctc gcgtgtgccc gtcgtcattt cgccctcaac
     1801 tccacctcgg tgatctcttc cttcaggtcg cgccacagcc gatcgaagaa ctcagggaag
     1861 gctggcgcgg cgtggatgtg gaacacgtcg cgaggccgat ccatgggaac ctcgtagaca
     1921 tgcttgatcg tccccggtgc ccgactgaac aggacgatcc tgtccgagag ggcgatggct
     1981 tccaccaagt cgtgggtcac gaagatgatc gtcttgccgg aaccgtccca cagcttcagc
     2041 aactcatcct gcaggatgac tcgtgtctgg gcgtcgagcg gcccgaaggg ctcgtccatc
     2101 agaatgatgt ccggctcgta gatcagggtt cgcaccagcg cagcccgctt gcgcatccca
     2161 ccggaaagct cgtggggata atggttctcg aagccgctca gacccacttt ggcgatatac
     2221 gtcgccgcct gctcgtggcg
//
LOCUS       DBNGZC010000528         3810 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2000438, whole genome shotgun sequence.
ACCESSION   DBNGZC010000528 DBNGZC010000000
VERSION     DBNGZC010000528.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2000438"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(429..902)
                     /locus_tag="AC16G0_09200"
     CDS             complement(429..902)
                     /locus_tag="AC16G0_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR5099825.1"
                     /translation="MKPRLDHTVAPDGYKAMLGLETYVRRSGLEPSLLELVRLRASQI
                     NGCAYCIDMHTKDARAGGESEQRLYALDAWRETPFYTDRERAALAWTEAVTQVAEGHV
                     PDEVYELARQHFNEKELADLTLGVIAINGWNRLAISFRTVPGTYTPASPPRKATA"
     gene            complement(1018..1245)
                     /locus_tag="AC16G0_09205"
     CDS             complement(1018..1245)
                     /locus_tag="AC16G0_09205"
                     /inference="COORDINATES: protein motif:HMM:NF038399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Os1348 family NHLP clan protein"
                     /protein_id="HIR5099826.1"
                     /translation="MSQAAVERALGKLMTDRLFREKFFEDPARAVSFAGLDLSEAELK
                     ALSRLPASAVSRFSLFLDDRIARLPLEEQPA"
     gene            complement(1346..1600)
                     /locus_tag="AC16G0_09210"
     CDS             complement(1346..1600)
                     /locus_tag="AC16G0_09210"
                     /inference="COORDINATES: protein motif:HMM:NF038399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Os1348 family NHLP clan protein"
                     /protein_id="HIR5099827.1"
                     /translation="MSQAAVERALGKLVTDEAFRESFFHDPATASFHAGLELSTAELD
                     ALLRLSAKAIATFSARLDARICRLRLEERGQTRSVKGSGG"
     gene            complement(1904..>3810)
                     /locus_tag="AC16G0_09215"
     CDS             complement(1904..>3810)
                     /locus_tag="AC16G0_09215"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR5099828.1"
                     /translation="LLLHEEHYTTAFDPQGRYVVADWAIRRVFAGEAVLVHEFASDPR
                     FASVNPHERKLQEAGIRSGIGVPLKSGGRVIGALAATSLTPHRYEETHLDTARQVADL
                     IAPFIQSKVLLQRERRRRGRLRALGTLTQVLATSLDARDVFARFAEAVKPVLDFDLMG
                     AVLISTSGRDLELLAKAGLPDMKGPPERIPLEHYSFTRRVEAGEAVLIQDTAVELDPT
                     LPGDRRILDGGGRSCMVVPLRFGEKVGGGLFFGKRRPYWFDALDVELATGIASQVVVA
                     VQHQRLAEDQKRLTLAEGRARQLEQRLTTLRQELGDRYGFDRILGRAPALQDALARAA
                     KVAPTETTVLLTGESGTGKELVARAIHYASPRAEGPYVAINCAALPETLVESELFGHE
                     RGAFTGADKQKPGRFEAAAGGTLFLDEIAELPTSAQAKLLRVLQEREFQRVGGTTTLR
                     ADVRLITATNQDLAQAVAEGKFREDLFYRLNVFSVHLPPLRERGEDILLLAQHFVREL
                     APKLGKGSGGLSRDAQEVLRSYHWPGNIRELANAVERALILAGGELLTAAHFGLARVK
                     HQDLRGTGGPPGEPSTPEPLVDLERRAIRAALERTKGNKTRAAALLGISRTQLHTRLR
                     RFGLAPESAA"
BASE COUNT          605 a         1285 c         1240 g          680 t
ORIGIN      
        1 gatccggagg gtcgagagat cgggacctgc gggccgctcc cgcatctgtg cctccggcac
       61 cccgtgattg cgttggagga tttccccccg cgacactcct cagggggaac gataggcctt
      121 gccctcacct ttggtgactc aggcctcagg ctcgtgcctg cgtggaatcg tcgagataga
      181 gtattgcgcg gcatttcacg tgtcaacaat gggtttgtcg ggtggaactc ttgcggtcgc
      241 tcgggccctc ccaccgcagg tgatcaggcc gactggcacc gcagggcagg ggcatcgttg
      301 ccatccggga gatcactgca atgccccgcg tcggtctgat gaagagcaac cgcgtcgacg
      361 tcctgaacgg gctactgaaa acggtccatc tgcagcattg gcgtgcgcct atcgcgcagg
      421 accatcactc acgccgtggc ctttcgaggt ggggaagccg gggtatacgt tcccggcacg
      481 gtgcggaagc tgatggcgag tcgattccag ccgttaatgg cgatgacgcc cagggtcagg
      541 tccgccagtt ccttttcatt gaaatgctgc cgggccagct cgtacacctc gtccggtacg
      601 tgtccctcgg cgacctgcgt gactgcctcg gtccaggcga gcgccgctcg ctcgcggtcc
      661 gtatagaacg gcgtctcccg ccaggcatcg agggcgtaca ggcgttgttc gctttcgccc
      721 ccggcccggg cgtccttcgt gtgcatgtcg atgcagtagg cacagccgtt gatctgggat
      781 gctcggagcc tcaccagttc cagcaaggag ggctcgagac cgctccggcg aacgtacgtc
      841 tccagtccga gcatcgcctt gtaaccgtcg ggcgcaaccg tgtggtcaag tcggggtttc
      901 attgtatctt ctcgcgacgt catcgaatcc tcctgttgtc actcatcctc attgccccgc
      961 caggccggga ccgaactaca tgctctgccc gtttgccgtt catcctgcca cgtcccctca
     1021 cgccggctgc tcctccagtg ggaggcgggc gatccgatcg tccaggaaca ggctgaaacg
     1081 gctgactgcc gaggcgggga gtcgagacag tgccttcaac tccgcctccg agagatcgag
     1141 acccgcgaaa gagacggccc gtgccggatc ctcgaagaac ttttcgcgga atagcctgtc
     1201 tgtcatcaac ttaccgagcg ctctctccac cgccgcttga ctcatgggtg ctcctcctga
     1261 tttggacctc tctcggccag aataacgcca cacgacggcg gacagcttcg ctcgcgggcc
     1321 tcgacccgaa ggctcgcgcg tcctgttacc cgccggatcc cttcaccgac ctcgtctggc
     1381 ccctctcttc gagacggagc cggcagattc tggcatccag ccgcgcactg aatgtcgcga
     1441 tcgctttcgc cgagagtcgc agcagagcgt ccaattctgc cgtggacagt tcaagaccgg
     1501 catggaagct cgccgtcgca gggtcgtgaa agaacgactc acggaaggct tcgtccgtca
     1561 ccaatttgcc cagggctctc tcgaccgcag cttgactcat cagcctcgcc cctgcccttc
     1621 gaaatcccct ctccacgggg tggtctcgcg tgtgggtcgc aattgccaca tgcccccgca
     1681 caggacctcc aagctgcagc tcaggtcgcc atagtgctga tccgtcagga gcctcgacgc
     1741 gtgcgctttt tacaaacgcc ggaatgcagc cgcggtgcca atctccgcca gcggccccac
     1801 gccttgaaaa gcctcacttg gacaagacgc ccgccgggtg agatcggggg aggtgtctat
     1861 cagtgagcag acggctgatc agcgatggac aggtcgtgca ggatcaagcg gccgactccg
     1921 gggcaagtcc gaagcgtctc aggcgggtgt gcagttgcgt ccggctgatc ccgaggagcg
     1981 ccgcggctcg cgtcttgttg cctttggtgc gctccagggc ggcgcgaatg gcgcgccgtt
     2041 ccaggtcgac aagcggttcg ggcgtcgatg gctccccggg gggaccgccg gttcctctta
     2101 gatcctgatg cttcacgcgg gccagcccga aatgggccgc cgtcaggagt tcgcctccgg
     2161 ccaggatcag ggcgcgctcg acggcgttcg ccagctcccg gatattgccg ggccagtgat
     2221 aggaccgcag cacctcctgg gcgtcgcggc tcaggccgcc gctgcccttt ccgagcttgg
     2281 gggccagctc tcggacgaag tgctgggcca ggaggagaat gtcctcaccc cgttcgcgca
     2341 gcgggggcag gtgcacgctg aagacattga ggcggtagaa gagatcctca cgaaacttgc
     2401 cctccgccac ggcctgggcc agatcctggt tggtggcggt gatcaggcgg acatccgcgc
     2461 gcagggtggt ggtccccccc acgcgctgga actcgcgctc ctgaaggacc cggaggagct
     2521 tcgcctgggc gctcgtgggg agctcggcga tctcatccag gaagagcgtt cccccggcgg
     2581 ccgcctcgaa gcggccgggc ttctgcttgt cggcgcccgt gaaggccccc cgctcgtggc
     2641 cgaacagctc ggattccacc agcgtctcgg ggagggccgc gcaattgatg gcgacatagg
     2701 gcccctcggc gcgcgggctg gcgtaatgga tggcgcgggc caccagctcc ttccccgtcc
     2761 cgctctcgcc cgtgaggagc accgtcgtct cggttggggc caccttggcg gcgcgggcga
     2821 gcgcgtcctg gagggccgga gcccggccga gaatccggtc gaagccgtat cggtcgccca
     2881 gctcctggcg gagggtggtg agccgctgct ccagttgacg cgcccgcccc tcggccagcg
     2941 tcaggcgttt ttgatcctcg gccaatcgct ggtgctgcac cgccaccacg acctgggagg
     3001 cgatgccggt ggccagctcg acatccagcg cgtcgaacca gtaggggcgg cgcttcccga
     3061 agaagagccc gccacccacc ttttcaccga accggagcgg cacgaccatg caggagcggc
     3121 cgccgccatc gaggatccgc cgatcgcccg ggagggtcgg gtccagctcg accgccgtgt
     3181 cctggatgag caccgcttct cccgcttcca cccgccgcgt gaaggagtag tgctccagcg
     3241 ggatccgctc cggggggccc ttcatgtcgg gcagacccgc cttggccagc agctccaggt
     3301 ctctcccgct ggtggagatg agcaccgccc ccatgaggtc gaagtcgaga accggcttca
     3361 ccgcctcggc gaaccgggcg aagacgtcgc gggcgtcgag actcgtcgcc agcacctggg
     3421 tgagtgtccc tagcgcccgc aggcggcccc ggcggcggcg ctcacgctgg agcaggacct
     3481 tgctctggat gaacggcgcg atcaggtcgg ccacctgccg ggccgtatcc aagtgggttt
     3541 cctcatagcg atgcggcgtg agactcgtgg ccgcgagagc cccgatgacc ctgccgccgc
     3601 tcttcaacgg gactccgatg cccgagcgga ttcccgcttc ttgaagttta cgttcgtggg
     3661 gattcacgct cgcgaagcgg gggtctgacg cgaattcgtg caccagcacc gcctcgccag
     3721 cgaagacgcg gcgaatcgcc cagtcggcaa ccacgtagcg gccttgcggg tcaaaggcgg
     3781 tggtatagtg ctcctcgtgg agcaggaggc
//
LOCUS       DBNGZC010000529         2175 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2100204, whole genome shotgun sequence.
ACCESSION   DBNGZC010000529 DBNGZC010000000
VERSION     DBNGZC010000529.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2100204"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="AC16G0_09220"
     CDS             complement(<1..885)
                     /locus_tag="AC16G0_09220"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR5099829.1"
                     /translation="MKGLVLEAKWEPKAGYVVSDFEKKTGKAITGNSVYKSPSLQVKE
                     VPVPKIGPKDVLLKVKACGVCGSDIHMYETDAQGYVMYPGLMKLACITGHEFSGVVQE
                     VGGEVRDLKPGDMVTAEEMIWCGECKPCRAGFVNQCTHLEEIGFTINGAFAEYIAIGS
                     KYCWKLDSLLERYSDPDLVYDLGATVEPTSVAYNAVFVRGGGFKPGAYTVVYGGGCIG
                     LTAIAMMKASGAAKVIAFETVAERRKLCTTMGADHVYDPIALTKQGTRASDVILDLTH
                     GEGADFQLEAAGAPALTFP"
     gene            1307..>2175
                     /locus_tag="AC16G0_09225"
     CDS             1307..>2175
                     /locus_tag="AC16G0_09225"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR5099830.1"
                     /translation="MIRVATAPMNWNSDDLADLRPTIPIRQVLAEMVEAGYEGTEYGT
                     GLPDDPQELKAILSPYGLALASMFCWIRLEDPARQRDEIERALAIARTLGAMDVHELI
                     LGIRGSERRIELAGRVPRDGSAGLTEAQWHVLADGIHALARSCEPLGVRLAVHPHAGS
                     YVETRDELETLFRVTDSRALGLCVDAGHLVYGGGDPVEVVRTFGSRVRYVHIKDVDPD
                     VLARCRREGLSFLEALRLYVFCGLGKGSVDLRGFVGALQHAGFSGWMVIEEDTSPDPP
                     LVAARRNRRYLKDT"
BASE COUNT          366 a          693 c          724 g          392 t
ORIGIN      
        1 cgggaaggtc agggcgggcg cccccgccgc ctccaactgg aaatccgctc cctcgccgtg
       61 ggtgagatcc aggatcacat ccgacgcccg ggtgccctgc tttgtcaggg cgatggggtc
      121 atagacatgg tccgcgccca tggtggtgca gagcttccgc cgctcggcta ccgtttcgaa
      181 ggcgatgacc ttggcggcgc cggaggcctt catcatggcg atggcggtga ggccgatgca
      241 gcccccgcca tacaccacgg tgtaggcacc tggcttgaag ccgcccccgc ggacgaagac
      301 cgcgttgtag gccacgctgg tcggctccac cgtggcgccg aggtcataca cgaggtcggg
      361 atcggaatag cgctccaaaa gggaatccag cttccagcag tacttgctgc cgatggcgat
      421 gtactcggcg aaggccccat tgatggtgaa gccgatctcc tccagatgcg tgcactgatt
      481 cacaaagccg gcgcggcagg gcttgcactc gccgcaccag atcatctcct cggccgtgac
      541 catgtctccg ggcttgaggt cgcgaacctc gccccccacc tcctgcacca cgcccgagaa
      601 ttcgtggccg gtgatgcagg ccagcttcat cagaccgggg tacatcacgt agccctgggc
      661 gtccgtctcg tacatgtgga tgtcggaccc gcagacgccg caggccttca ccttgagaag
      721 gacatccttg ggcccgatct tgggaaccgg gacttccttc acctgcaggc tgggtgactt
      781 gtacacgctg ttcccggtga tggccttgcc cgtcttcttc tcgaaatcgc tgacgacgta
      841 ccctgccttg ggctcccact tcgcttccag caccagtcct ttcatcccac gcctccttct
      901 cttcgcagtg cccaccgctc caccggcggc aaacagtaac ccgtttcctc ctctcacatc
      961 cggccatacc caagaggaag agtccactct gggctctact ttcgtgggag tcccgacccc
     1021 tgtcaaggag ggaatggcaa cctggagact ttgttttgag tgcgggacgt taccgctaac
     1081 agactcttgg tcgacgggag cgccgaaacg ggagagaaca gccagcccga atagcgcgag
     1141 acgccgattc gaacgcccgg cgggccttgc gtcctccgga cacgtcggct aatctaggct
     1201 gccctgagag cggaccggcg cgggagagac tgggctggat cggtcatgcc cggcatcggg
     1261 ctccgagtcg tcgaccggag ccgggctgat gtatgggagg gaacgcatga ttcgtgtggc
     1321 gacggcgccg atgaactgga atagcgacga cctggcggat ctccggccga cgattcccat
     1381 ccggcaggtc ctggcggaga tggtcgaggc cggctatgaa gggaccgaat acggcacggg
     1441 ccttcccgat gatccgcagg agctcaaggc catcctgagt ccctacggac tcgcgctggc
     1501 ttcgatgttc tgctggatac ggctggaaga tccggcccgg cagcgggacg agatcgagcg
     1561 ggcgctggcc atcgcccgga cactgggggc gatggatgtc cacgagttga tcctgggcat
     1621 ccgggggagc gagcggcgca tcgagttggc tggacgggtg ccgcgggacg gcagcgccgg
     1681 tctcacggag gcgcaatggc acgtcctggc cgacgggatc cacgccctgg cacggtcgtg
     1741 tgagccgctt ggcgtacgac tggcggtcca cccccatgcg ggctcttacg tggagacgcg
     1801 ggacgaattg gagacgctct tccgcgtcac ggacagccgc gccctgggtc tgtgcgtgga
     1861 tgcgggacac ctcgtgtatg gcggcggcga tcctgtcgag gtcgtcagga ccttcgggtc
     1921 gcgggtgcgg tacgttcaca tcaaggacgt ggatcccgac gtcctggcgc ggtgtcgccg
     1981 tgaaggtctg agctttctcg aggccctgcg cctgtacgtc ttctgcggcc tggggaaggg
     2041 aagcgtggac ctccggggat tcgtcggggc gctgcaacat gcgggattct cgggctggat
     2101 ggtcatcgag gaggacacgt ccccggaccc cccgctcgtg gccgcgcggc gaaatcggcg
     2161 atacttgaag gacac
//
LOCUS       DBNGZC010000530         1755 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_631719, whole genome shotgun sequence.
ACCESSION   DBNGZC010000530 DBNGZC010000000
VERSION     DBNGZC010000530.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_631719"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="AC16G0_09230"
     CDS             <1..224
                     /locus_tag="AC16G0_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007090901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HIR5099831.1"
                     /translation="ARAMESSPTGRPVAWQNPDSGHSGTVTPTRTYQTAQGAYCREYE
                     TTVLIDGKKERATGTACRQPDGTWRVVNS"
     gene            380..784
                     /locus_tag="AC16G0_09235"
     CDS             380..784
                     /locus_tag="AC16G0_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024890.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HIR5099832.1"
                     /translation="MRQSLCWALIVATALVVFAGPADAQAPVRPDWKESFRMHDKNGD
                     GRIDRAEFQNWMVDTFYFRDTNHKGYLVSSDLQGVRAPELIKAMNRQSDGKLTLSEFL
                     NALFQDFAAIDVNGSGSITIEEIQAYIQRNGK"
     gene            904..>1755
                     /locus_tag="AC16G0_09240"
     CDS             904..>1755
                     /locus_tag="AC16G0_09240"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR5099833.1"
                     /translation="MNDASRDLARIMLQVLFIGALIAGSFWILRPFLLASLWAATIAV
                     ATWPLMLLVEKRLGQRRGLAVTVMTLALLMALLVPLALATIAIIQNADRLVGWARALP
                     AFSIPPPPEWLGRVPLVGSKLVAKWGQVSALGPEGLSTYVTPYARVLVGWFVAQVGGV
                     GILLLEFLLTIIIAAIMYSNGEKVAAGTRRFARRLGGPQGESVVDLSVQAVRSVALGV
                     VVTALVQSALAGIGLAIAGIPFAPILAGVILVLSISQIGPAPVLIPAVVWLYWTGGSG
                     WGTFLLVW"
BASE COUNT          296 a          555 c          558 g          346 t
ORIGIN      
        1 ccgcgcgggc catggagtcg tcgcccacgg ggagacccgt cgcctggcag aatcccgata
       61 gcggccactc gggaacggtc accccgaccc ggacctatca gaccgcccag ggcgcctact
      121 gccgggagta cgagaccacc gtgctgatcg acggcaagaa agagcgggcc accgggaccg
      181 catgccggca gcccgatggc acctggcggg tcgtgaactc ctagatctga gccgacactt
      241 cctcgataat tcgaggattg aaggcgttca gcgatcagct ttcagcaagc gctgatgact
      301 gacggttgaa cgcttgccgt gtattttcgg cctggggttt accaggaccc acaccccgac
      361 agcgggggag gaggatcaca tgcggcaaag tctctgctgg gctttgattg ttgcgacggc
      421 gctcgtcgtc tttgccggtc ccgccgatgc ccaggcgccc gtgcgacctg attggaaaga
      481 gagttttcgg atgcacgaca agaacggcga cgggcggatc gaccgcgcgg aattccaaaa
      541 ctggatggtg gacaccttct acttccggga tacgaaccac aagggctatc tggtctcctc
      601 ggatctgcag ggcgtgcgcg cgcccgaact gatcaaggcg atgaaccgcc agagcgatgg
      661 gaaactcacc ttgagcgaat ttcttaatgc gctcttccag gatttcgcgg cgatcgatgt
      721 caacgggagc gggtcgatca cgatcgagga gatccaggcg tacattcagc gcaacggaaa
      781 gtgagcctga gatttggtgt gagtggcaca ggctgaaacc cgtcctcgat acggcccggt
      841 ctttgccctg cccggggacc cgtcagtcgt gcataagcag gcacggaagg gacgtgacaa
      901 gccatgaatg atgcctcccg cgacctcgcc cgtatcatgc tccaggtgct cttcatcgga
      961 gcgttgatcg ccggcagctt ctggatcctc cgtcctttcc tcctggcctc cctctgggcc
     1021 gcgaccatcg ctgtggccac ctggccgctg atgctcttag tggaaaagcg gctagggcag
     1081 cgtcgcggtc tggccgtgac ggtcatgacg cttgccttgc tcatggcgct gctcgtgccc
     1141 ctggcacttg ccactatcgc tatcatccag aacgctgacc ggcttgtcgg ctgggcgagg
     1201 gccctaccgg ccttcagcat tccgccgccc ccggagtggc tgggccgggt cccgctggtt
     1261 ggatcgaaac ttgtggcgaa atgggggcag gtttccgcgc tgggcccaga aggtctctcc
     1321 acatacgtga cgccctatgc aagggtgctc gtgggctggt tcgtagcgca ggtcggcggc
     1381 gtggggatac tcctgctgga attcctcctg accatcatca tcgccgccat catgtacagc
     1441 aacggcgaga aggtcgccgc cggcacgcgc cgcttcgccc gccggcttgg cgggccccag
     1501 ggtgagagtg tcgtggacct atccgtccaa gcggtccgtt ccgtcgccct cggggttgtc
     1561 gtcaccgctc tcgtccaatc ggccctggca ggcatcggct tggccatcgc cggcatcccc
     1621 tttgccccaa tcttagcggg tgtcatcctg gtgctctcga tttcgcagat tggccccgcc
     1681 cctgtcctca tcccggcggt ggtgtggctg tactggacgg gaggatccgg ctggggaacg
     1741 ttcctgctcg tctgg
//
LOCUS       DBNGZC010000531         7128 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1807188, whole genome shotgun sequence.
ACCESSION   DBNGZC010000531 DBNGZC010000000
VERSION     DBNGZC010000531.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7128)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7128)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7128
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1807188"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..468)
                     /locus_tag="AC16G0_09245"
     CDS             complement(43..468)
                     /locus_tag="AC16G0_09245"
                     /inference="COORDINATES: protein motif:HMM:NF012899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid kinase family protein"
                     /protein_id="HIR5099834.1"
                     /translation="MSKLVVLAIGGNAFIVVAAGGGIPVVRDADGRLRGVEAVVDKDL
                     TAALLARGLQADILLIATAVEQVFVDYGKTTQRPIERITRGEAGALMEQGHFAPGSMQ
                     PKVRAAIDFLDHGGKMAIITSLRTLAPALRGQGGTVITG"
     gene            complement(538..1749)
                     /locus_tag="AC16G0_09250"
     CDS             complement(538..1749)
                     /locus_tag="AC16G0_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005497889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HIR5099835.1"
                     /translation="MAIDYKRVLEKAEEYKPAISRFLRDLIALPSESCQEREVIRRIK
                     QEMESVGFDRVDIDPMGNVLGTIGLGRHLVAMDAHIDNVGVGNPDNWRFDPFKGYEDD
                     EVIGGRGASDQKGGMASLVYAGKIIQDLGLTADYTLLVTGTVQEEDCDGLCWQYLVEK
                     RRIRPEFVVSTEPTSCRVHRGQRGRMEIKVSVRGVSAHGSAPERGDNAIFKMAPILGE
                     LQRLHARLAEDAFLGKGSLTVSEIFFTSPSRCAVADGCAISIDRRLTNGESWEGALEE
                     IRRLPAVQAARAQVSLYDYSRPSYTGLVYPTECYFPCGVVEEKHPACQALVAAYQSLF
                     AKKPVVDKWTFSTNGVSIMGRYGIPCVGFGPGHEDQAHAPNELTWKSELVQAAAMYAV
                     IPQVYLTGLLP"
     gene            2178..5837
                     /locus_tag="AC16G0_09255"
     CDS             2178..5837
                     /locus_tag="AC16G0_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011032122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR5099836.1"
                     /translation="MSLKSPFSLRSWLALLVVGVAVPLIAFAGLTIWSILDANEQERD
                     KDLSARARAIASAVDAETRSWRAALYALAENKNLRAGRLGEFYHEAQAVAARYEGWVV
                     INDATGQQRLNTLLPLGAPLPHTSAGEMVTAVLRDGKPLTDLVYGAAAQRYIIANAVP
                     VFREGKVALCLNMNFYPERLTRLLEEQKLPPTWVAAIVNHQNRVVSRSIKAEERVGKL
                     APQALQQAVAQSDMGLVNYALTDGRRSRVAFQHLREVPWALALAVPFAELPSPRHLIG
                     FVVIGILLAGVAVGAAVAVARKIAQPISRLAHLAPCLVRSEAAGIDVAPRIAEVRQLQ
                     QALVEAAEQVQAHAEERERAAEGLRRSSAYTRSLIEASLDPLVTIGPDGTITDVNAAT
                     EAATGQLRSALIGTDFSDYFTEPARARAGYEQVFRDGFVRDYPLELRHRDGRVMSVLY
                     NASVYRDETGKVQGVFAAARDITERKRAEDIIRLQADQYRAMLATTSDGFWLYDTEGN
                     LLDVNDVYCRMSGYSRDELLHMRISEIEAIESPQEVARHIRQVIEGTGFDRFESQHRR
                     KDGSLWDVEISATYWRTTGQMLVFCRDITQRKQAEEALRRLTEELEARVQRRTAALQE
                     AHVKLEAANTQTQEANKRLEEMNAELQQEVEERQRVEEELANANAALTNERNLAAQRA
                     DELEVVFAAMHDAVEVHNTDGVMVKANAEALALYDFDPIGMDRKTRNTKNGLRYLDGS
                     RVPLDALPTCRAERGEWFHDERFLIYHAGHGADRVVQMSAAPLYKDQQLIGTVVLWQD
                     MTEHEKMLQQLRDHQRILEETVQARTAELAQTVKILTAEVSRRTLAEEGLRDRSEQLR
                     LLASELTIAEQRERQRVATVLHDDLQQLLVGARLVISPLLQAADPGVQAMGREAMDLL
                     ALSLQCSRSLTEELSPPILHRGGLRHALEWLVRWMADKHHLTVHVHTDDAAAVEPPDM
                     TLLLFQAIRELLFNVTKHAQTQSAWVTITHRDAQIQVQVADEGAGFEPTLLRVAGGTA
                     GGFGLFSLRERLELLGGRLEIESAPGKGSRFTLLTPVKASTPLETPEAPPLSRRQQPA
                     DPDVSDWKRTRVLVVDDHVIVRQGLVRLLNAESDLEVVGEASDGDEAVELTGRLRPDV
                     VVMDVDLRELNGVEATRAIHAEFPEMQVIGLSMFEEAEKGPAMREAGAANYLSKLGPS
                     EALLAAIRACVRSDA"
     gene            complement(5911..6798)
                     /gene="dapF"
                     /locus_tag="AC16G0_09260"
     CDS             complement(5911..6798)
                     /gene="dapF"
                     /locus_tag="AC16G0_09260"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR5099837.1"
                     /translation="MRAVPFTKMSGVGNDFILVDNRAGILQEAGLQEFARRVCTRRMS
                     LGGDELMVIEPPTAGGDFSMRTFNPDGGEVKMCGNASRCVARYAYVHGIAGARMRIDT
                     LGGPVDAWVEGDEVRVKLQLTSPIELDHALTVQGESHIAHTLHVSGAPHAVVYLRDVD
                     RASGEAIHRLGAAIRHHAHFPQGINVNFVQVIDRHTLWQRTFERGVEGETLACGTGAV
                     ASSVISSLRGQVDTPVRLRVLGGELSVSFRREEDSFDELFLGGGARFVAEGVLHPEAW
                     CWPEAEPMARATPGSVVIP"
     gene            complement(6898..>7128)
                     /locus_tag="AC16G0_09265"
     CDS             complement(6898..>7128)
                     /locus_tag="AC16G0_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR5099838.1"
                     /translation="EREAIALMARSEGILLDPVYTGRAMAGMADLIRKGEFGKDETIV
                     FWHTGGSAALFAYADQLLAASKAQGELRNFPG"
BASE COUNT         1266 a         2320 c         2318 g         1224 t
ORIGIN      
        1 attggtcatt ggtcatttga tccggactta ccccaacggc cctcagcccg tgatgactgt
       61 ccccccctgg ccgcgcaagg cgggggcgag cgtccggagt gaggtgatga tggccatctt
      121 tcctccatgg tccaagaaat cgatggccgc ccggaccttg ggctgcatgc tgcccggagc
      181 aaagtgaccc tgctccatca gggcaccggc ctcgccgcgg gtgattcgct ctatcggccg
      241 ctgagttgtc ttgccgtagt ccacaaagac ctgttcgacc gcggtggcga tcaggagaat
      301 gtcggcctgg agtccccggg caaggagcgc cgcggtgagg tccttatcca ccaccgcctc
      361 cacgccccgc aggcgcccgt ccgcgtctcg caccacgggg atgccgccgc cggccgccac
      421 cacgatgaag gcattcccgc cgatggccag gaccacgagc ttggacaagg tcacttcact
      481 ccgcggccgg gcatccctcg ccggccatcg aatggccaag gcgcacgcga ccctgcctca
      541 cggcagcagc ccggtcagat agacctgcgg gatcacggcg tacattgccg cggcttgcac
      601 cagctcgctc ttccaggtga gctcgttggg cgcatgggcc tggtcctcgt ggccggggcc
      661 gaagcctacg cagggaatac cgtagcggcc catgatggac acgccgttgg tggagaaggt
      721 ccatttgtcc accaccggct tcttggcgaa cagggactgg tacgccgcca ccagcgcctg
      781 gcaggcgggg tgcttttcct cgaccacccc gcaggggaaa tagcactcgg tggggtagac
      841 caggccggtg tatgaaggcc gggagtagtc gtagagggac acctgggcgc gggccgcttg
      901 cacggcgggg aggcgtcgga tctcctccag ggcgccttcc caagattcac cgttggtgag
      961 gcggcggtcg atagagatgg cgcagccgtc ggccacggcg cagcggctgg gcgaggtgaa
     1021 gaagatctcg gacacggtga gggatccctt gcccaggaag gcatcctcgg ccagccgagc
     1081 gtgaagcctc tggagctctc ccaggatggg ggccatcttg aagatggcgt tgtccccgcg
     1141 ctccggcgca gatccgtggg cgctgacgcc ccgcacggac accttgatct ccatgcggcc
     1201 ccgctggccg cggtggacgc gacaggaggt gggctcggtg gagaccacaa actcgggccg
     1261 gatgcggcgt ttctccacga ggtactgcca gcagaggccg tcacagtcct cttcctgaac
     1321 ggtgccggtg acaaggaggg tgtaatcggc cgtgagaccg agatcctgga tgatcttgcc
     1381 agcgtagacg agagaggcca tgccgccctt ctgatcgctg gccccccggc cgccgatgac
     1441 ctcgtcgtcc tcgtagccct tgaagggatc aaatctccag ttgtcggggt tgcccacacc
     1501 cacgttgtcg atatgggcgt ccatggcgac gaggtgccgt ccgaggccga tggtgcccag
     1561 cacattgccc atgggatcga tgtccacccg gtcgaagccg actgactcca tttcctgttt
     1621 gatccgccgg atgacctccc gttcctggca gctctcgctg ggcagggcga tgaggtcccg
     1681 gaggaaccgg gagatggccg gcttgtattc ttcagccttt tcgagaaccc gtttgtagtc
     1741 gatggccacg atcgtcctcg cgtgtgttgg ctggtatctc ctcggcgctt caatcgcagg
     1801 caggctgaga tccgcttggc agagtgccgt ctattatcac aaagctcttg cttccgcaat
     1861 gcatcgggag agctttgtct agcacttcgg cagtccccgg ttcggcagcg caaccctctc
     1921 acggacgcga ctgtgctgac gatcaatgcc cctgaggcat tcctggtgat gcgccctccg
     1981 cattgacagc gctgatgggc tggcgtagag tccaacctgc atcacctacc tccagtccga
     2041 gggtgcgggc gccgggtccc aaccgaactt ctcccgcgaa gaacaggccg atggcaggcc
     2101 ctttgcataa ctgttcctcg ccgtgtgtag tcgtctgact ccctaaggca gctcctgtgg
     2161 ccttcccggg aggtgcgatg agcctgaaat cgcctttcag cttgcgttcc tggcttgccc
     2221 tactcgtggt cggcgttgcg gtccccctga tcgccttcgc gggcctgacg atctggtcga
     2281 tcctcgatgc caacgagcag gagcgagata aggacctgag cgcgagggcg cgcgccatcg
     2341 cctctgccgt cgacgccgag acccgctcgt ggcgcgcagc cctttacgcc ctcgccgaga
     2401 acaagaatct ccgagcggga cggctcgggg agttctacca cgaggcacag gccgtcgccg
     2461 cgcgctatga aggctgggtc gtcatcaacg atgccacagg acagcaacgt ctcaacacct
     2521 tacttccgct gggtgcgccc ctcccgcata cttctgccgg ggagatggtg acagcagtcc
     2581 tccgggatgg aaaacccctc acggacctgg tctatggggc ggccgcgcag cggtacatca
     2641 tcgccaacgc cgtgcccgtg ttccgcgagg ggaaggtggc actttgcctg aatatgaact
     2701 tctatcccga gcggctgacg cggctcttgg aggagcagaa actgccccca acctgggtgg
     2761 ccgccatcgt aaaccaccag aatcgtgtgg tgtcccgctc catcaaagcg gaagaacgcg
     2821 tcggaaaact ggcgccgcaa gccctgcaac aggcggtagc ccagtcggat atggggcttg
     2881 tgaattacgc cttgaccgac ggtcgaaggt cccgcgtggc gtttcagcac ctcagggagg
     2941 tgccctgggc gctggccttg gccgtcccct tcgccgagct gccttcgccg cgccatctta
     3001 tcggtttcgt ggtgatcggc atccttctgg ccggggtcgc ggtcggcgcg gcggtcgccg
     3061 tcgctcgcaa gatagcccaa ccgatcagcc gcctggctca cctggcgccc tgcctggtgc
     3121 ggagtgaagc tgcggggata gacgtcgcac cccgcattgc ggaggtacgc cagttgcagc
     3181 aggcgctggt cgaggcggcc gagcaggtcc aggcgcacgc ggaggaacgg gagcgggcgg
     3241 cggagggttt gcgtcgctcg agcgcctaca cgcgcagcct catcgaggcg agtctcgacc
     3301 ccttggtgac catcgggccg gatggcacga tcaccgacgt gaacgccgcc accgaggccg
     3361 ccaccgggca actccgatcg gcgctgatcg gaacggactt ctcagactac tttaccgagc
     3421 cggcgcgcgc ccgggcaggg tacgagcagg tgttccggga tggattcgtc cgcgactatc
     3481 ccctggagct ccggcaccgg gacggacggg tgatgtccgt cctctacaac gcctccgtct
     3541 accgcgacga gaccgggaag gtccagggcg tatttgccgc cgcccgggac atcaccgagc
     3601 gcaagcgggc ggaggacatc atccggctgc aggcggatca gtaccgagcc atgctcgcga
     3661 ccaccagcga cggcttttgg ctctacgata ccgaaggcaa tctcctggac gtcaacgacg
     3721 tctattgtcg gatgagcggt tactcccgag acgagctgct gcatatgcgc atctcggaga
     3781 tcgaggccat cgagtcgcct caggaggtgg cccgacacat ccgccaggtc atcgagggga
     3841 caggcttcga ccgtttcgag agtcaacatc ggcggaagga cgggagcctc tgggatgtcg
     3901 agattagcgc cacctactgg cggaccacgg gccagatgtt ggtgttttgt cgcgacatca
     3961 cccagcggaa gcaggcggaa gaggccctcc gccggctcac cgaggagctg gaagcacgcg
     4021 ttcagaggcg caccgcggcc ctgcaggagg cccacgtcaa actggaggcg gccaataccc
     4081 agacgcagga ggcaaacaag cggctggagg aaatgaacgc agagttacag caggaagtcg
     4141 aggagcggca gcgagtagag gaggagcttg ccaacgccaa cgcggcgctg acgaatgagc
     4201 ggaaccttgc agcccagcgc gcggatgaac tggaagtggt atttgcagcc atgcacgatg
     4261 ccgttgaggt tcacaatacc gatggggtga tggtgaaggc gaatgcggag gccctggcgc
     4321 tctatgactt tgaccccatc ggcatggatc gaaagaccag aaacacaaag aacggtcttc
     4381 gctatctgga cggcagcagg gtcccgttgg acgcgcttcc cacctgtcgt gccgagcgag
     4441 gagaatggtt tcacgacgag cggtttctta tctaccatgc tggccacggt gccgaccgcg
     4501 tcgttcagat gtccgccgcc cccctctaca aagatcagca gctcatcggc accgtcgtgc
     4561 tgtggcaaga tatgaccgaa cacgagaaga tgctccagca gttgcgagat caccagagaa
     4621 tcctggagga gacagtccag gcacgaaccg ccgagctggc ccagacggtg aagatactca
     4681 cggccgaggt cagccggcga acgctggccg aggaggggct ccgcgaccga tcggagcagt
     4741 tgcggctcct ggcctccgag ctgaccattg ccgagcagcg ggagcggcag cgggtggcga
     4801 cagtgctgca tgacgacctc cagcagctcc tggtgggagc gaggctcgtg atcagccccc
     4861 tacttcaggc cgcggaccca ggggtgcagg caatgggccg ggaggcgatg gacctgctcg
     4921 ccctgtccct ccagtgctcg cggtccctca cggaggaact cagcccgccc attctgcatc
     4981 gaggtggtct gcgccacgcg ctggaatggc tggtccggtg gatggcagat aagcaccacc
     5041 tcacggtcca cgtccacacg gatgacgcgg ccgccgtgga gccccccgat atgaccctcc
     5101 tgctcttcca ggccatccgg gagctgctct tcaatgtcac gaagcacgcc cagacccaat
     5161 ccgcctgggt caccatcacg cacagggacg cccagattca ggtccaggtc gcagacgagg
     5221 gcgcgggctt cgagccgaca ctgctccgcg tggccggagg caccgcaggg ggttttggcc
     5281 tgttcagcct gcgcgagcgc ctggagttgc tgggcggccg gctagagatt gagagtgctc
     5341 ctggcaaagg cagccggttc acattgctga cccccgtgaa agcctcgacc cccctggaaa
     5401 cgcccgaagc acctccgctg tcccggcgtc aacaaccggc tgaccccgat gtgagcgatt
     5461 ggaagcgcac ccgtgtcctc gtggtggacg atcacgtgat cgtccgccaa ggcctggtca
     5521 ggctgctcaa cgcggaatcg gatctcgaag tcgtggggga ggcgtcggac ggcgatgagg
     5581 ccgtggagct gaccggccga ctccggcccg atgtggtcgt catggacgtg gatctgcggg
     5641 aactgaacgg cgtcgaggcc acccgggcca tccacgccga attccccgaa atgcaggtga
     5701 tcggcttgtc catgtttgag gaggcggaga aaggcccggc gatgcgggag gccggtgcgg
     5761 cgaactatct tagcaagctg gggccgtcgg aggcgctgct cgccgctatc cgtgcctgtg
     5821 ttcggtcgga cgcgtaacct cttggcccgg ccttcttgcc agcgttgacc gctccctcgg
     5881 agcgatgagc cccgcagccg accccaggtt tcaggggatc actacggacc ctggagtggc
     5941 gcgagccatc ggttccgcct ctggccagca ccaggcttct gggtgcaaca cgccctcggc
     6001 cacaaaccgc gcgccgccgc ccaggaacag ttcgtcgaag ctgtcctcct cgcgcctgaa
     6061 ggaaacgctc agttcgccgc cgagcacgcg tagccgaacg ggggtgtcca cctgcccccg
     6121 caaagaactg atgacgctcg aggcgacggc tcccgtgccg catgccagtg tctcgccttc
     6181 cacgccgcgc tcaaaggtgc gttgccagag cgtgtgcctg tcgatcacct ggacgaaatt
     6241 gacattgatg ccctgaggaa aatgggcgtg atggcggatg gccgccccca ggcgatggat
     6301 tgcctctccc gacgcccggt caacatcccg caggtacacc acggcgtgcg gtgcgccgga
     6361 gacgtgcagc gtgtgggcaa tgtggctttc cccctgaacc gtcagcgcgt gatccagttc
     6421 gattggcgag gtgagctgca atttcacgcg cacctcgtcc ccctcgaccc aggcatccac
     6481 cggaccgccc agggtatcga tcctcatcct ggctccggcg atgccgtgga cgtaagcata
     6541 gcgggccacg cagcgggacg cgttgccgca cattttgact tcgccgccgt ccgggttgaa
     6601 ggtgcgcatg gaaaaatcgc cgccggctgt ggggggctcg atgaccatca gctcatcgcc
     6661 gcccagcgac atccgccgcg tgcagacccg gcgggcaaat tcctgcagcc ccgcctcctg
     6721 caggatacct gcgcggttgt ccacgaggat aaagtcgttg ccgactcccg acatcttggt
     6781 gaaggggacg gctcgcatgc aggcttcctc tcatgtgatt cgacgagcgc caggggtctt
     6841 ccgacgcttc gccatgccat ggcagcgtcc cacgcgccgg acgccgaaga ccccacgcta
     6901 accgggaaag ttgcgtaact ccccttgggc ttttgacgcc gccagcagtt gatcggcgta
     6961 ggcgaagagc gcggccgagc cgcccgtgtg ccagaagacg atcgtctcgt ccttgccgaa
     7021 ctcgcccttg cggatgagat ccgccatccc ggccatcgcg cgtcccgtgt agacggggtc
     7081 cagcaggatg ccctcgctcc gcgccatcag agcgatggcc tcccgctc
//
LOCUS       DBNGZC010000532         1881 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2686291, whole genome shotgun sequence.
ACCESSION   DBNGZC010000532 DBNGZC010000000
VERSION     DBNGZC010000532.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2686291"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="AC16G0_09270"
     CDS             <1..379
                     /locus_tag="AC16G0_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5099839.1"
                     /translation="LCIDDDQLVLSVCYDALVDHGYRVLTERDGPTGLATAKAEQPDL
                     ILLDVIMPGMDGLEVCRRLRAEPSLTATPIILLTAFPHPEVAAEGRQAGATLTMRKPY
                     GPELIASTVNQALGRKPRPRRAL"
     gene            459..707
                     /locus_tag="AC16G0_09275"
     CDS             459..707
                     /locus_tag="AC16G0_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099840.1"
                     /translation="MSQKKTELYRGFNIHTGEVRTGVWGCSVVEIPSSEEAGINRTRH
                     QGHVSGEFQSKESALEAARTHIDRIHKNRLNRANQTGG"
     gene            760..882
                     /locus_tag="AC16G0_09280"
     CDS             760..882
                     /locus_tag="AC16G0_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099841.1"
                     /translation="MSRKSDAIGRKVPRSTYERDRRSILKAVPKLRKIKPRKGR"
     gene            968..1405
                     /locus_tag="AC16G0_09285"
     CDS             968..1405
                     /locus_tag="AC16G0_09285"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR5099842.1"
                     /translation="MTEPRALPVRADFSFWWPVTVRWGDMDAMGHVNNMMYLQYLESA
                     RVGYFEAMGWGGRLVNGRKQGPVVVSQTFNYRRQVVYPDELEVGVHCQEIRNRSFILA
                     YGVFRKGTDLLVGDGDTVTVWLDYGDGKAVEIPSSLREALAAN"
     gene            complement(1675..>1881)
                     /locus_tag="AC16G0_09290"
     CDS             complement(1675..>1881)
                     /locus_tag="AC16G0_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR5099843.1"
                     /translation="SRFWPKDQYKPGGPQPSFDKQFLRDYLLSIKWPQAPPPPPLPAE
                     IVTRTREKYLEAYTRLLGKPATFA"
BASE COUNT          376 a          535 c          642 g          328 t
ORIGIN      
        1 cctctgcatt gatgatgacc agcttgtcct cagcgtttgc tacgatgcct tggtggacca
       61 cggctaccgg gtcctcacgg agcgggacgg ccccacgggg ctcgccacgg ccaaggctga
      121 gcaaccggac ctcatcctcc tggacgtcat tatgcccggg atggacggac tggaggtctg
      181 ccggcgcctg cgggccgagc ccagtctcac ggcaaccccc atcatcctcc tcacggcctt
      241 cccccatccc gaggtggccg ccgagggccg ccaggcgggg gccaccctca ccatgcggaa
      301 accctacggc cctgagctga tcgccagcac cgtgaaccag gccctcggcc ggaagccccg
      361 accccggagg gcgctgtgag cgggggatcc tgtaagggtc tcatggccgt cttgggccag
      421 catcaggcga gggcgtcggg ggtacgaagg ggcaggaaat gagtcaaaag aagacggagc
      481 tgtatcgagg cttcaacatt cacacgggag aagtccggac cggggtctgg ggatgctcgg
      541 tggtcgagat tccctcatcg gaagaggcgg gcatcaaccg cacgcgccat caggggcatg
      601 tgtcgggaga gtttcaatcc aaggagtcgg cactggaggc ggcaaggacg catatcgatc
      661 ggatccacaa gaacaggctg aacagggcca accagaccgg aggatgaata tgcgacgacg
      721 cctcagtatg cgcccagcgg gcgcttaccc aaagtcctca tgagtcgcaa atcggatgca
      781 attggccgga aggttcctcg aagcacctac gagcgggatc gtcggagcat cctgaaggcg
      841 gttccaaagc ttcgcaagat caaaccgcgc aagggacggt gaagtccggc gaggctgcgg
      901 cctcgccggc gcaatcaacc caggccatcg tctccgcgac cgtggggacg aggagggatc
      961 actgtcgatg acagagcctc gggcgttgcc cgttcgtgct gacttctctt tctggtggcc
     1021 cgtgacagtc cgctggggtg acatggatgc catgggtcac gtgaacaaca tgatgtacct
     1081 gcaatacctc gagtcggccc gggtcggata tttcgaggcc atgggatggg ggggccggct
     1141 cgtgaacggt cgaaaacaag gcccagtggt cgtgtcccag acgttcaact accggcgcca
     1201 ggtggtctat ccggatgaac tggaggtggg cgtccactgt caggaaattc gaaatcgaag
     1261 cttcatcctg gcgtatgggg ttttccggaa agggaccgac cttctggtgg gggacgggga
     1321 caccgtgacc gtctggctgg actacgggga cggtaaggcg gtcgagatcc cgtcgagcct
     1381 gcgtgaagcc ctggcggcga attaaggccg aaaatctcac ggtcgagatc accaagaacg
     1441 ccaagaattg atcggcacgc tctgggaaag agcctcggat tccgaaggct ccgagagggg
     1501 cggttccgaa atctcgattt gtagacggaa tatccctctg tttggcttcc ttggcgcttt
     1561 ggcggtttaa tgattcttct gaatcaggct gcacagacgc cacgggcgag aggtagggga
     1621 gcgcccccgg tctgaatgat ggcctgctag cgaccgagag cggtcggtac gaggctatgc
     1681 gaaggtcgca ggcttgccca ggagtcgggt gtaggcctcg agatacttct cccgcgtcct
     1741 ggtgacgatc tcggccggca agggcggggg agggggcgcc tgcggccact tgatggacag
     1801 gagatagtcg cggaggaact gcttgtcgaa ggacggttgg ggtccgcccg gcttgtactg
     1861 atccttgggc cagaaccggg a
//
LOCUS       DBNGZC010000533         3384 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2489264, whole genome shotgun sequence.
ACCESSION   DBNGZC010000533 DBNGZC010000000
VERSION     DBNGZC010000533.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2489264"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="AC16G0_09295"
     CDS             complement(<1..326)
                     /locus_tag="AC16G0_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HIR5099844.1"
                     /translation="MTRMWTSWHLLPFVVVVGLAGCATVPAGPSEMVLPGEGKPFEQF
                     QAEDADCRQWANTQVGTTPGQAWTESALSHATIATLLGAALGAAIGAASGDAGIGAAI
                     GAGSGAL"
     gene            2241..2630
                     /locus_tag="AC16G0_09300"
     CDS             2241..2630
                     /locus_tag="AC16G0_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099845.1"
                     /translation="MAKVLPPQKKPITYGTEVRSVPKNPQSKATLCEMAEEQRRSTQG
                     QIDQLRPTPQPVSGLTERILAKIAAEEADRKLAAQPQLHPALPVDVKQIDRAMATTVA
                     GRAHWRLAAISGLLLGIAVASFWASFP"
     gene            complement(2872..>3384)
                     /locus_tag="AC16G0_09305"
     CDS             complement(2872..>3384)
                     /locus_tag="AC16G0_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR5099846.1"
                     /translation="NVLAYLAQAASGIYDPAADLDAVLMPERVAAYRIFLHSACVAVT
                     ATVNEEIERTANPKERAELVGWKDLLPVEVLNAPADAIAQRARWFQRYHPELQDCQIV
                     AEAEIGGLEVLLTFDRTLRRRLDGRSPTLRLRSPSEHWRELQIPRGQPPRWSPTPTSP
                     IADATWWRWE"
BASE COUNT          636 a         1136 c         1013 g          599 t
ORIGIN      
        1 agggccccgc tcccggcccc gatggcagcg ccgatgcccg cgtcacccga ggcggcacca
       61 attgcggccc ccagggccgc cccgaggagg gtagcgatgg tggcatggct cagggcgctt
      121 tccgtccagg cctgtccggg cgtggtgcct acctgggtgt tggcccactg ccgacagtcg
      181 gcgtcctccg cctggaactg ctcgaagggc ttcccctcgc cgggcagcac catctcgctc
      241 ggaccggcgg gaaccgtggc gcagccggcc aggcccacga cgacgacgaa ggggagcaga
      301 tgccaactag tccacatgcg tgtcatcttt cctactccta ctgcgatggc ccgctgggcg
      361 gcgctgtctg ggacgggacg acggtcaacc agccggaggc gcattccttg acatacgggt
      421 agtaggtgcg ggaatccttg cagtagtacc agtagttggg tggcgcgggt tcccgctgga
      481 tatactcagg cggggacggc gggtccaccg ctgatggcgt atagtagggg tagccatagg
      541 aaggatagcc gtagtacgga tagccgtaag agtaacctgg ccctccccac caccaggagc
      601 cgccccacca atagccggtg ccgatgacaa ctccaacgcc gcccccgccg tgcccgtgat
      661 gacctccacc tgaatgccag ccgcctgagg ggtgccccgt tcctccgtgc cacgagcccc
      721 cgcctccatg ccacgtgcct gtggaatgcc cagcgcgagc gccggcgtgt cctgagccgc
      781 tggctccacg ccacgtgcct gcggagtgcc cagtccctcc gtgacccgac cccccgcctc
      841 cgcgccatgc gcctgaggat tgaccgccac gagagccgcc ttgccgtgaa ccgctggctc
      901 ctggggcgac ttccggtgtc aggccgagca cgagagcggc agtcatcagg actgcagcca
      961 ccagcccttt ccgcatcttc tttctccttt cgtgggcctc ccggcgcccc gacgagccaa
     1021 ccgctccttc ggcatgccac cgtcgacaat aatatatacc acgcctgctc ctgcggacag
     1081 atcatacgcc acggcttcgc acattcgact ccgatgaaac cgtcaaccgc gtagcccttg
     1141 ttcaaaggaa tcttgccagc attccttttc ttacaaacag atttacaggt tattgacaga
     1201 tttgcagata gagcatatac gttttgcccg cctcgaccga aaatccccaa cctttctcgc
     1261 cactccgacg tgggtaatcg ccgggaattc ctggatctct caccgcctgc tctactctgc
     1321 tccgatccac tccgcacttt ggttaccgtg cggcatgaag cttgctgtct gtaagtgaga
     1381 tacctgcaat ttatcacggg tcaacgcagc cacagcacat gattttcggg gtcacctgag
     1441 cggaaacaga atgctgaggg agactctgcg atctaggcca gtcggttcgc accccggcgc
     1501 gggtcgacgg cggctgctcg cgaacctgag ccgcgacccg tcgcctcagg tggtgccccc
     1561 ctccttctca cctcagccta ggtacgctcc gggtggtgtc gagggccgag attttcgggg
     1621 gagagaagac cgagcgattc cgaggagagg atacgatggc cccgcaaccg ccaggcactt
     1681 tcaagtacga tctgcagtcc atcacgcaga acgcctcacc ggcctccggt gtatacgcgc
     1741 tcttctcgcc gagcgaatgc gtttttgtcg gggagtccga cgacgtttgt gccagcctcc
     1801 tggagacctt ctatgaggac aaccccagcc tgaatggcca gtacattacg cacttctcca
     1861 ttgaactggt ttccccggaa tgccgagtgg cacgccaggt cgaccgcatc cgggagctga
     1921 ggccggtctg caatggggga atgggctccc ccgagtgtgg ccacagccgc agcccacagg
     1981 ggcagagcgg aagtgaagct gtccaccttg cggagcatca tggccgggag gcggcttccc
     2041 caagttcccc gaggccatgt attgggtctc ccagggctct ggaggtcacg ccggcagccc
     2101 agggcggcct gaggccgcca gaccctccag gtcaggcacg caccgttgag ggtccgcaag
     2161 tccgaaagga ggagaaagcc tccgatcact cgacgggtca actgcccaca ggatctcgtc
     2221 ccgaaatccc gcttgcatca gtggcaaagg tcttgccgcc acagaagaag ccgataacct
     2281 acgggaccga agtgaggagc gtccccaaga acccccagtc caaggcaacg ctctgcgaaa
     2341 tggccgagga gcagcgtcga tcgacccagg gccagatcga ccagcttcgc ccgacccctc
     2401 agccagttag cggcctcacc gagcgcatcc ttgcgaagat cgcagcggag gaggcagatc
     2461 ggaagctcgc cgcacagccg cagctccacc cggcactccc agtggacgtg aagcagatcg
     2521 atcgcgccat ggccacaacc gtggcgggac gggcgcactg gagactcgcc gcgataagcg
     2581 gtttgctcct cgggattgcg gtcgcgtcat tttgggcgag cttcccgtga gagattctgg
     2641 aagccactcg cgttgtgttc ccgctaggca attgagcttt accgcgacgt cccaaagagt
     2701 tcttctgccg gcatgggcat cccccgaagg gtcagcagga gctctcaagg tgcacggcac
     2761 gaacctcccg ccggacccac gtctccacat cactgcacgg gatatggaca gcgagaccag
     2821 ccggtttgga accctcgaac cactgccgca tgcgccccag ccgctccgct ttcattccca
     2881 tcgccaccac gtggcatcgg cgatgggact cgttggggtc ggcgaccaac ggggcggctg
     2941 cccccgcggg atctgaagtt ccctccagtg ttctgagggg gatcgtaggc gcaacgtggg
     3001 cgagcgaccg tcgagtcgtc ttcggagcgt ccggtcaaac gtcagaagga cctccaggcc
     3061 accaatttcc gcctcggcaa caatctgaca gtcctgcagc tcggggtgat acctctggaa
     3121 ccaccgagcc cgctgggcaa tcgcgtccgc cggagcattc aagacctcga cagggagaag
     3181 gtctttccac cccaccagtt ctgcccgctc tttgggattg gcggttcgtt cgatttcttc
     3241 gttgacggta gccgtgaccg caacacatgc tgaatggagg aagatccgat acgcggcaac
     3301 ccgctcgggc atgagaacgg catccagatc ggctgctgga tcatagatgc cgctcgccgc
     3361 ctgggcgagg taggcgagca catt
//
LOCUS       DBNGZC010000534         1483 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_240490, whole genome shotgun sequence.
ACCESSION   DBNGZC010000534 DBNGZC010000000
VERSION     DBNGZC010000534.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1483)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_240490"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1483)
                     /gene="glmS"
                     /locus_tag="AC16G0_09310"
     CDS             complement(<1..>1483)
                     /gene="glmS"
                     /locus_tag="AC16G0_09310"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 -
                     L-glutamine:D-fructose-6-phosphate transaminase
                     (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR5099847.1"
                     /translation="HNGIIENYRELRRSLAARGHRFRSETDTEVIAHLIESYRTNGLP
                     EAVVRASRELRGAYALACIADDTPDALVAVRAGSSPLVIGFRDGEAFLASDIPALLGE
                     TRDILVVEEGEMAVLTPRGITLRTLDGAPVRRTPTTIPWDCEAAQRSGFPHFMLKEIF
                     EQPETVRNTLRGRADPDTGEIEIPEMGLKDRELVGLNRVCFVGCGTSWHAALVGKYLV
                     EEFARLPVEVDVASEFRHRRPVMDSRVLTVPISQSGETADTLGAVCEVKGYGSRIVAI
                     CNTVGSSVARQADGVIYTRAGIEIGVASTKAFAAQLTAVTLLALKLGLARGLADRAAV
                     REVVKGLWEIPDLLAALLARSDQIREIAGRFADRTKFLYLGRGIHYPLALEGALKLKE
                     ISYIHAEGYPAGEMKHGPIALVDKRVAVVSIVPAGPTYEKMTTNLEEVKAREGVVLAV
                     ANEGDEAIADKADAVMPIPAAAYWVQPLLVALPLQLLAYHLAVS"
BASE COUNT          258 a          503 c          464 g          258 t
ORIGIN      
        1 ggagacggcc aggtgatagg cgaggagctg aagcggcaac gcaaccagca acggttgaac
       61 ccagtacgcg gcagcaggga tgggcatcac cgcatccgct ttatccgcaa tcgcctcatc
      121 cccttcattc gccaccgcca gtacaacccc ttctcgcgcc ttcacctcct cgaggttggt
      181 cgtcatcttt tcgtaggtcg gcccggccgg aacgatggag accaccgcca ccctcttgtc
      241 gacgagggca atgggtccat gcttcatctc tcccgccggg tagccttcgg cgtggatgta
      301 ggagatctcc ttgagcttga gtgccccctc cagggccagc gggtagtgga taccccgccc
      361 caggtagagg aatttcgtgc gatcggcgaa tcggccggcg atctcgcgga tctgatcgct
      421 ccgagccagc agggccgcca gcaggtcggg gatttcccac agacccttga cgacctcgcg
      481 gacggccgcc cggtccgcca gtccccgggc gaggccgagt ttcagggcca gaagggtcac
      541 cgccgtgagt tgggccgcga aagccttggt cgacgccacc ccgatctcga tgccagcccg
      601 ggtgtagatc accccatccg cctggcgggc cacggagctt cccaccgtgt tgcagattgc
      661 aacgatccgg gagccgtacc ccttgacctc gcacacggcg cccagcgtgt ccgcagtctc
      721 accggattgg gagatgggaa ccgtcaggac ccggctgtcc atcaccggtc gccgatgccg
      781 gaattcggaa gcgacatcca cctcgacggg aagtctggca aattcttcca ccagatattt
      841 ccccaccagc gccgcgtgcc aggaggtacc gcagcccaca aagcacaccc gattcaggcc
      901 caccagctcg cgatccttga gtcccatctc ggggatctcg atctccccgg tatccgggtc
      961 ggcccgtccc cgcaacgtgt tccgaaccgt ctcgggctgt tcgaagatct ctttgagcat
     1021 gaagtggggg aagccggacc tctgggcggc ctcgcagtcc catgggattg tggtcggcgt
     1081 ccgccgtacg ggagcgccgt ccagggtgcg aagcgtgatt ccgcgggggg tgaggaccgc
     1141 catctcgcct tcctccacca cgagaatatc gcgcgtctca cccaggagcg ccgggatgtc
     1201 cgaggcgagg aaggcctccc cgtcccgaaa tcctatgacc aggggggagc tgcccgcacg
     1261 aacggccacg agcgcatccg gggtatcgtc agcgatgcag gcgagggcgt atgcgccccg
     1321 gagttccctg ctcgcccgga ccactgcctc gggcaggccg tttgttcgat aggattcaat
     1381 gaggtgggcg atcacctccg tgtcggtctc ggagcgaaag cgatggcccc tggccgccag
     1441 agaccgtcgg agctcacggt agttctcgat gatcccgttg tgg
//
LOCUS       DBNGZC010000535         2102 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1513422, whole genome shotgun sequence.
ACCESSION   DBNGZC010000535 DBNGZC010000000
VERSION     DBNGZC010000535.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1513422"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..527
                     /locus_tag="AC16G0_09315"
                     /pseudo
     CDS             63..527
                     /locus_tag="AC16G0_09315"
                     /inference="COORDINATES: protein motif:HMM:NF015655.7"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
     gene            603..>2102
                     /locus_tag="AC16G0_09320"
     CDS             603..>2102
                     /locus_tag="AC16G0_09320"
                     /inference="COORDINATES: protein motif:HMM:NF015655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase family
                     protein"
                     /protein_id="HIR5099848.1"
                     /translation="MYRQYLDYAALDEIRAMKDRINLAVAQDRRARRHVKLGYGGIRE
                     IEFLVQGFQLLHGGKNPWVRETNTLRALHRLAGQGLLADQDYEALVRAYSFLRKLEHR
                     LQIIHDRQTHTLPEEPRDLLGLARRMGYHPLEHSDPVAALLREYEQHTTAVRTLYDTF
                     LQGMQPREGEPEEGSADELTLFFSADLSVEQLRERLASVGFEDLDRALRNLAMIREGQ
                     PFAHYPPEAHRALARLAPGLVEALRGVPDPDLALTTFERFLAMVAARTTYVGLLADSP
                     GLLTLLIQLFGTSEFLSATLLHHPDLLDALLAPEPATAHGRGRVGADLANAMAEAPTG
                     SARLDALRRVKKAEELRIGLRDILNQADVTQTHRALTHLADACLEAALRLAEQDLEER
                     FGTAEPAGFAIVAMGKLGGMELGYASDLDLLFVYLQEGTTTGPERLSHPEYFSRLADR
                     VTKILTSITQEGAAYRVDARLRPWGQKGELALPLEAFETHFTRMADPWER"
BASE COUNT          335 a          692 c          745 g          330 t
ORIGIN      
        1 gcttcaaggc ccgggagatc ctgcggatcg gcctccagga ccttttgggc cggcagatcc
       61 tggtgggtgt cacggaggag ctgtccaatc tggccgacgt ggtgctagaa accgcctacc
      121 gtctctgccg ggcggaggtc gcccgccggc acgggcagcc ccagctcgag ggcgaggccc
      181 ctcggacccg ggagtgccgg ttcgtgatct tgggcatggg gaagctggga gggcaggagc
      241 tgaatttcag ctccgacatc gacctcatct tcgtctacga gggggagggg gagaccaccg
      301 gagtccccgg acccgacggg acccgggtgg ggcgggtgag caaccaggag ttcttccgga
      361 agctcggcga gatgctggtg aaaggggtga gcgaggcatc gcccgatgga cacctgtacc
      421 gcgtcgatct ccgcctccgc cccgagggcc ggacggcggg catcgcctcg acgctgcggg
      481 cctgagagat ctattacgag gcctggggac agacctggga gcgtcaggcg ctgatcaaga
      541 cccggcctgt tgccggggat cccacgctgg gggaggcctt cctgcgcctg atcgtcccct
      601 tcgtgtatcg tcagtacctg gactacgccg ctttggacga gatccgtgcc atgaaggacc
      661 ggatcaacct ggcggtggcc caggatcggc gcgcccggcg acacgtgaag ctgggctacg
      721 gcggcattcg cgagatcgag ttcctggtgc aggggttcca gttgctgcac gggggcaaga
      781 acccttgggt gcgggagacc aacacgctgc gcgccctgca ccgattggca ggccagggtc
      841 tgctggcgga tcaggattac gaggccctgg tccgggccta cagcttcctc cggaaactcg
      901 agcaccggct tcagatcatt cacgaccggc agacccacac gctccccgaa gagccgcgcg
      961 acctcttggg gctggcccgg cgcatggggt accacccgct ggaacactcg gatccggtgg
     1021 ccgcgctgct ccgcgagtac gagcagcaca ccaccgccgt gcggactctg tacgacacct
     1081 ttctccaggg gatgcaaccg cgcgagggcg agccggagga ggggtccgcc gacgaattga
     1141 cgttgttctt cagcgccgat ctctccgtgg agcaactgcg ggagcgcctg gcctccgtgg
     1201 gattcgagga tctggatcgg gccctgcgga acttggcgat gatccgcgag ggccagccct
     1261 tcgcccacta cccgcccgag gcgcaccgcg cgctggcacg tctcgccccc gggctggtgg
     1321 aggcgttgcg gggggtcccc gatcccgacc tggccctgac caccttcgag cggtttctgg
     1381 ccatggtggc cgcacggacc acctacgtcg ggctgctggc cgactctccg ggcctcctga
     1441 cgctgctgat tcagctcttc ggcaccagtg agttcctctc ggctacccta ctgcaccacc
     1501 cggacttgct ggatgccctc ctggcaccag agccggcgac cgcccacggc cggggtcggg
     1561 tgggtgcgga cctcgcgaat gccatggccg aggcgccgac aggcagcgcc aggctggacg
     1621 cactgcgccg ggtgaaaaag gcggaagagc tgcgcatcgg tctccgggac atcctgaacc
     1681 aggcggacgt gacgcagacg catcgagcgc tcacccacct ggccgatgcc tgtctggaag
     1741 ccgccctcag gctggcggag caggatctgg aggagcgttt cgggacggca gagccggcgg
     1801 gtttcgccat cgtggccatg gggaagctgg gcggcatgga gttgggctac gcatcggacc
     1861 tggatctcct cttcgtgtac ctgcaagagg ggaccacgac cggccccgag cggctgagcc
     1921 accccgagta cttcagccgc ctggcagacc gcgtcaccaa gattctcacc tccatcaccc
     1981 aggagggcgc ggcctaccgg gtggacgcgc ggctgcggcc gtgggggcag aagggggagc
     2041 tggccctgcc gctggaggcc ttcgagacgc acttcacccg gatggccgat ccgtgggagc
     2101 gg
//
LOCUS       DBNGZC010000536         7190 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1609062, whole genome shotgun sequence.
ACCESSION   DBNGZC010000536 DBNGZC010000000
VERSION     DBNGZC010000536.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7190)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7190)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7190
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1609062"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="AC16G0_09325"
     CDS             complement(<1..1259)
                     /locus_tag="AC16G0_09325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR5099849.1"
                     /translation="MTDRRVAVTGMGIVTSIGIGVEPYWKAALSGCSGVGPVRHFDAS
                     GLPAQIASYVHDHVTLEAWRVRLGLRPGDPRALLFALAAGEMAYEACGWDPRLAGDRV
                     GVVFGTYGDKVHMGRLAEIAYRSRPEGSQEISPASFFSAYCRMQNGHQLYRLMPHYAT
                     AALARRYAATGPTCTIQTACTSSAQAIGEAYRAVRRGTVERAICGGAECIVTPDQMVM
                     FSLLGVLSTRNDEPERASRPFDGKRDGFVLGEGSGVLVLERMDLALERGAPILCELAG
                     YGTSCDAYRLTDEDPEGAGAVLAMRRALESAGLDPGHIDYINAHGTSTPMNDRIETLA
                     IKTVFGKGAYDIPVSSTKSMVGHTVSAAGAIEAISCILAIRDQILPPTINYEERDPQC
                     DLDYVPNFARPAGLAVALSNSFGFGGH"
     gene            complement(1272..2048)
                     /gene="fabL"
                     /locus_tag="AC16G0_09330"
     CDS             complement(1272..2048)
                     /gene="fabL"
                     /locus_tag="AC16G0_09330"
                     /EC_number="1.3.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008881553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabL"
                     /protein_id="HIR5099850.1"
                     /translation="MRALAGIRQVLKGKVAVVTGGSRGIGRAISLRLAAAGANVVVNY
                     FLNREAAEKTAAEIRRHGVRAHLVQADMKDPRQIRALFDAVQGEFGGLDILVSNAASG
                     VFRPVQELSAKHWDWMMNTHPRAFLLCAQAAVDLMQGRQGRIVCISSIGSVRAIPQYA
                     GMGASKGALETLTRYLAVEFAPHGIAVNCVSGGAVATETWQQLPEVEMVIEGIRRKTP
                     AGRIATPEDIAEAALFLVSPQAEMIRGQVLIVDGGFSLTL"
     gene            complement(2014..2532)
                     /locus_tag="AC16G0_09335"
     CDS             complement(2014..2532)
                     /locus_tag="AC16G0_09335"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HIR5099851.1"
                     /translation="MRFLLVDRILRLEEGKEAVILKNVSNSEDFFADHFPGRPIMPGC
                     LILESCDQAARLLLAKSTGFQRLPRLEQVANGKFRYFVQPGDSLQVHVSVVSQNADGA
                     TVRAAASVGDRSVAQASLTYSLVDAAGDPGATRACAQLREFVEALSTDVLSGVMESRA
                     RDAGPGGDPASA"
     gene            complement(2562..3173)
                     /locus_tag="AC16G0_09340"
     CDS             complement(2562..3173)
                     /locus_tag="AC16G0_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012759.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR5099852.1"
                     /translation="MTGQTPELREWSRNEVEAALRNILVESLGVKDEEVVASASVVRN
                     LGAESIDFLDVSFQIQQTFGVNIQAAEIRDRIIAWGALILPTLLEVLEGRYGVKLSAE
                     TLRPLEASGIEGVLAHLRVTHGISIESGAADGVGRELLRRLIKEFTAFGFVVSEADER
                     DLLAIMRADLGTRRITERTMDLLTVGSLANFICAKLGPRLRAD"
     gene            complement(3246..3992)
                     /locus_tag="AC16G0_09345"
     CDS             complement(3246..3992)
                     /locus_tag="AC16G0_09345"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5099853.1"
                     /translation="MQVNLGGKLALVTGGSRGIGAALVRAMAKAGAAVVVNYREDESA
                     ARQVVEEIRGVGGDASLWQADVRDGQAVSAMIEGIVGRAGRLDILVNNAGIVRDALVP
                     EMGDEEWRSVLDTNLSGVFQCSRLAARHMLRKRWGRIINLSSVGAWRGNKGQGNYAAS
                     KAAVNALTRVMATELGPRGVTVNAVAPGLIDTAMASGVLPFADEFVREHIPLRRVGRP
                     EDVAPLVVFLASDHAAYITGQVFAVDGGLI"
     gene            complement(4110..5396)
                     /locus_tag="AC16G0_09350"
     CDS             complement(4110..5396)
                     /locus_tag="AC16G0_09350"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR5099854.1"
                     /translation="MLNRVVVSGVGMVSALGVGGGCHWTGLLSGRSAVSRVERLTRLG
                     FPVDVAAEVPPEKVAESLSRLSRKQLKLYNRATMLAMTAATLAAEDAGLTAPIAEGSR
                     AGVILGTLFIPYPIQSILRLLPGQEAPDDPFRMNMGRALKTSMKSVNPLDMSLKIVPN
                     LTGGHIAITYGLRGFCRTVSDGSTGGMHAIGQAMMAIREGELDVVFCGGTEAPLEDLV
                     FADLCATDLLAEPADIPERTCRPFGRGRKGMVAGEGAAVLVLEAEDHARQRGVRLRAE
                     IAGFGATIGERSLAGISASVERAMQAALADSQRDGVDLVSANGDGGRLNDQAEAGAIH
                     ACLETQSRPPAVYATKGAHGNLFSAAGPLEVATVVMALEQEVLPPSLNADDLDPECGL
                     RLSGNAPQALAGARTALVNALGAFGEATSLVVAKAS"
     gene            complement(5435..5938)
                     /locus_tag="AC16G0_09355"
     CDS             complement(5435..5938)
                     /locus_tag="AC16G0_09355"
                     /inference="COORDINATES: protein motif:HMM:NF019589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog family protein"
                     /protein_id="HIR5099855.1"
                     /translation="MHYQFIDRILEVDAHDAGRIVLVKTFPRTEDYFDGTFRRPDEVP
                     STLVLETMASAGALVLAVRSGYHANAALLKVNWARFPRPVYAGAELKVSLELTASQGD
                     WTGQADPRQAVGMAQALAQGFVGEAQVAEADMLFLCVPMPWVLGSGGEKVLTHYLELM
                     GIADARP"
     gene            complement(6017..6481)
                     /gene="acpS"
                     /locus_tag="AC16G0_09360"
     CDS             complement(6017..6481)
                     /gene="acpS"
                     /locus_tag="AC16G0_09360"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIR5099856.1"
                     /translation="MVALLPVRIPIPRPLAGRGRKAAVRETSAAAEPCIGVDLIAVSR
                     VREVFQGREALLESVFTPEEMRYCRRQKDPFMHLAARYAAKEAAVKGLGTGKTGRMSW
                     RDVETVHGALGEPRLVLRGEVARLVEAKGLHRCAVSLSHAGDYAMAAVLFTA"
     gene            complement(6510..>7190)
                     /locus_tag="AC16G0_09365"
     CDS             complement(6510..>7190)
                     /locus_tag="AC16G0_09365"
                     /inference="COORDINATES: protein motif:HMM:NF019240.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1571 domain-containing protein"
                     /protein_id="HIR5099857.1"
                     /translation="DLLRLLDLAEKSYASVQDYTSIMLGRERIGGILRPQETILLKFQ
                     RPFKVYMKWLEGPSKGREGLYVSGAREGKFLLYEPRGIRRLFTAALDPSDQRIMEQSR
                     HPVTDVGIGRLLEIVGENARRGTKSGVLQVTDRGIAELAGRRVHTLEGMLPRDPKLAY
                     YCYRVILSFDEENHLPIRVAVYDWDDQMVEEYIHTALQVNPGFTGRDFDPNNRDYGFS
                     GWRINISG"
BASE COUNT         1233 a         2605 c         2161 g         1191 t
ORIGIN      
        1 ttgtgacccc cgaatccaaa ggaattcgac agggcgactg ccaggccagc gggacgggcg
       61 aagttcggga catagtccag atcgcactgg gggtcgcgtt cctcgtaatt gatcgtgggc
      121 gggaggatct ggtcccggat ggccaggata cacgagatgg cctcgatggc gccggcggcg
      181 gagaccgtgt gcccgaccat ggatttcgtg gagctgaccg gaatgtcgta ggcgcccttg
      241 ccaaagaccg tcttgatggc cagggtttcg atgcgatcgt tcatcggggt cgaggttccg
      301 tgggcgttga tataatcgat gtgccccggg tccagccccg cgctttccag cgcccggcgc
      361 atggccagaa cggctccggc tccctcgggg tcctcatcgg tgagccggta ggcgtcacac
      421 gaggttccgt atccggccag ctcgcacagg atcggcgccc cacgctcgag cgccagatcc
      481 atccgctcca gcaccagcac gccgctcccc tctcccagga cgaatccatc ccgcttgccg
      541 tcgaacggcc ggctggcgcg ctcgggctcg tcgttccgcg tggacagcac acccaggagg
      601 gagaacatga ccatctgatc cggcgtcacg atgcattcgg ctccgccgca gatcgcccgc
      661 tccaccgtcc cgcggcggac cgcccggtag gcctccccga tggcttgggc ggagctggtg
      721 cacgcggtct ggatggtgca ggtcggcccg gtggccgcat accgccgggc cagggcggcc
      781 gtggcatagt gaggcatcaa gcgatacaac tggtgcccgt tctgcatccg gcagtaggcg
      841 ctgaagaagg aggcgggcga gatctcttga ctgccttcgg gccgagatcg ataggcaatc
      901 tcggcaagcc gacccatgtg gaccttgtct ccgtaggtcc cgaagacgac cccgacccgg
      961 tcgcctgcga gccggggatc ccatccacac gcctcgtacg ccatctcccc ggcagccagg
     1021 gcaaacagca gtgcgcgggg gtcccccggc cgaaggccga ggcggacgcg ccaggcctcc
     1081 agcgtcacgt ggtcgtggac gtagctcgca atctgtgcgg gcaatcccga cgcgtcgaaa
     1141 tgccggaccg gccccacccc cgagcaaccg ctcagggcgg ccttccagta cggctccacc
     1201 ccgatgccga tggaggtcac gattcccatg cccgtgacag cgacgcgtcg atccgtcatc
     1261 cgtcaatccc cttagagagt caaggagaag ccgccgtcca cgatcaggac ctggccgcgg
     1321 atcatctcgg cctgggggct caccaagaat agcgccgcct cggcgatgtc ctcgggtgtg
     1381 gcgatccggc ccgcaggcgt cttccgccgg attccttcga tgaccatttc cacctcgggc
     1441 agttgctgcc acgtttccgt ggcgaccgcc cctccggaga cgcaattcac ggcaatcccg
     1501 tgtggcgcga actccaccgc caggtatctg gtcagggttt ccagggcgcc cttggatgcc
     1561 cccatgccgg catactgggg gatcgcgcgc accgacccga tgctgctgat gcacacgatc
     1621 cggccttgcc gtccctgcat caggtccact gcagcctggg cgcacaggag gaacgctcgg
     1681 ggatgggtat tcatcatcca gtcccagtgc ttggcgctga gctcctgcac cggccggaac
     1741 acaccggagg cggcgttgct caccaagatg tccaggccgc cgaactcccc ctgcaccgcg
     1801 tcgaacagcg cccggatctg gcggggatcc ttcatatcgg cctgcaccag gtgggcccgc
     1861 accccgtgac gccggatctc cgccgccgtc ttctccgctg cctcccggtt gaggaaatag
     1921 ttcaccacga cgttggcgcc cgcagccgcc agccgcaatg aaatcgctcg accgatgccg
     1981 cggcttcccc ccgtcaccac ggcgactttt cccttaagca cttgccggat ccccgccagg
     2041 gcccgcatcc cgggcccggc tctccatgac gccgctgagg acatccgtgc tgagggcctc
     2101 gacgaattcg cgcaactggg cacaggcccg cgtcgccccg ggatcgccgg cggcatccac
     2161 cagcgaatac gtgagggagg cctgcgccac cgagcggtcg ccgacggaag cggccgcgcg
     2221 caccgtcgcc ccgtcagcgt tctgggacac cacgctcacg tgaacctgca gcgaatcgcc
     2281 cggctggaca aagtagcgga acttcccgtt ggcaacctgc tccaaccggg ggagtcgttg
     2341 aaatcccgtg ctcttggcca ggagcagccg ggccgcctga tcgcagctct ccaggatcag
     2401 gcatcctggc atgatcggcc gtccgggaaa gtggtcagcg aagaaatcct cgctgttgct
     2461 gacgttcttg aggatcaccg cttccttccc ctcctccagc cggaggatcc gatccaccag
     2521 aagaaagcgc atcagatggc gtcccctctg ccgtccatca atcaatcggc ccgcaggcgc
     2581 gggccgagct tcgcgcagat gaagttcgcc agcgaaccca ccgtcaggag atccatcgtc
     2641 cgctctgtga tccgacgcgt ccccaggtcg gcccgcatga tggccagcag atcccgctcg
     2701 tccgcctcgc tcacgacgaa accaaaggcc gtgaactcct tgatcagccg gcgcagcaac
     2761 tcccggccaa cgccatcggc ggcgccggat tcgatggaga tcccgtgggt cacgcggagg
     2821 tgggccagca cgccctcgat cccggacgcc tccagcgggc gcaacgtctc ggcgctcagc
     2881 ttcaccccgt agcgtccctc caggacctcc agcagcgtcg ggaggatcag cgctccccac
     2941 gcgatgatgc ggtcccggat ctcggccgct tggatgttga ccccgaaggt ctgttggatc
     3001 tggaagctca catccaggaa gtcgatggac tccgctccga ggttccgaac cacggacgcg
     3061 gaggccacga cctcctcgtc ctttaccccg agtgactcca cgaggatatt ccgcagcgcg
     3121 gcctccacct cgttgcgact ccactcccga agctccggcg tttgtcccgt catgagcgcc
     3181 cccggagccc gtcgccccgg ccaatcctca tgaggcgggc gggctcgtct gttcgaatcg
     3241 gcgaatcaga tcagtccccc atcaaccgcg aagacctgtc ccgtgatgta ggcggcgtgg
     3301 tccgacgcca gaaacacaac cagcggggca acatcctctg gccggccgac gcggcgcagc
     3361 gggatgtgct cccggacaaa ctcatcggcg aacggcaaca ccccgctcgc catggccgtg
     3421 tcgatcaggc cgggcgcgac ggcgttgacg gtgacccccc gggggcccaa ctcggtcgcc
     3481 atgacccggg tgagggcgtt caccgcggcc ttggacgccg cgtagtttcc ctgtcctttg
     3541 ttccctcgcc aggcacccac cgagctgaga ttgatgatcc gcccccacct ttttcgaagc
     3601 atgtgccggg ccgccagtcg cgaacactgg aacaccccgg aaaggttcgt gtccaggacc
     3661 gaacgccatt cctcgtcgcc catctcggga accagggcgt cccggacgat gccggcgttg
     3721 ttgaccagga tgtccaggcg gcccgcccgg cccacgatcc cctcgatcat ggccgagacg
     3781 gcttgtccgt cccgcacatc ggcctgccac agggaggcgt cgccgcccac cccacgaatc
     3841 tcctccacaa cctgccgggc cgcggattca tcctcgcgat aattcaccac caccgcggcg
     3901 ccggccttgg ccatggcgcg aaccagtgcg gcaccgattc cccgggatcc tcccgtcacc
     3961 aaagccagtt ttccgcccag gttgacctgc accgcggctc gcctttcccg cctcccggcc
     4021 cctcctcggg ctttcccccc ggacgacgcg ggtcgggccg gactatcgtg tgcgtcatgc
     4081 gccgcaccgt gtgtcaggta cccgccggct cagctcgcct tggcgaccac cagggatgtc
     4141 gcctcgccga atgcccccag ggcattcacc agggccgtcc gggcgcccgc cagcgcctga
     4201 ggcgcattcc cggagagccg caggccacac tcggggtcga gatcgtcagc gttgaggctg
     4261 gggggcagca cttcctgctc cagcgccata accacggtcg cgacctccag cgggccggcc
     4321 gcagaaaaga gattcccgtg ggcccccttc gtggcgtaca ccgcgggagg cctagactgc
     4381 gtctcgaggc aagcgtggat cgcgcctgcc tccgcctggt cgttcagccg gcctccgtcc
     4441 ccattggccg agaccaaatc caccccatcc ctctggctgt ccgccagggc cgcctgcatg
     4501 gcacgctcga cgctggcgga gatgccggca agagatcgct ctccgatggt ggccccgaat
     4561 ccggcgatct ctgctcgcag ccgcaccccg cgctgtctgg cgtgatcctc ggcttcaagc
     4621 acgaggaccg ccgccccctc ccctgcgacc atgccttttc ggccccggcc aaaaggacgg
     4681 caggtccgct cggggatgtc ggccggctcg gccagaaggt ccgtggcgca caggtccgcg
     4741 aacacgagat cctccagggg cgcctcggtc cctccgcaga agacgacgtc cagttcccct
     4801 tctcggatcg ccatcatggc ctggccgatg gcgtgcatac ccccggtcga cccatcagac
     4861 accgtccggc aaaatccccg gagaccgtag gtgatggcga tgtgtcctcc ggttaggttg
     4921 ggaacgatct tcaaagacat atccaggggg ttcacgctct tcatggacgt tttcagcgcc
     4981 cgccccatgt tcatccgaaa gggatcgtct ggagcttcct gccccggaag caggcgcaag
     5041 atactctgga tcgggtaggg aatgaacagg gtacccagga tgaccccagc gcgcgatccc
     5101 tcggcgatcg gggccgtcaa tcccgcatct tcggccgcca acgtagctgc ggtcatggcc
     5161 agcatcgtgg cccggttgta caatttcagt tgcttgcgtg agagcctgga cagactctcc
     5221 gccaccttct ccgggggaac ctcggcggcg acatccaccg gaaaaccgag gcgggtcaac
     5281 cgctccaccc gggacacggc cgatcggccg ctgagcaggc ccgtccaatg gcagcccccc
     5341 cccacgccga gggcagagac catccccaca cctgacacga cgacccgatt aagcatgtcg
     5401 caccggaggc ctcattcgca ggacagcccc ggtctcatgg ccgagcatcc gcgatcccca
     5461 tgagctccag gtagtgcgtg aggaccttct ccccacccga acccaggacc cagggcatcg
     5521 ggacacacag gaagagcatg tccgcctccg ccacctgcgc ctcaccaacg aagccttgcg
     5581 ccagggcctg cgccattccc acagcctgac gcgggtccgc ctggccggtc caatctccct
     5641 gggacgccgt cagttccagg ctgaccttca gctccgcgcc ggcatagacc ggccgcggaa
     5701 acctcgccca gttgaccttc agcagggcgg cattggcgtg gtagccactc cgcaccgcca
     5761 gaactagcgc gccggctgag gccatggtct ccagcacgag ggtagaaggg acctcgtccg
     5821 gtcgccgaaa ggtcccgtcg aagtagtcct ccgtgcgcgg aaaggtcttg acgagcacga
     5881 tcctgccggc atcatgggcg tcgacttcga gaatccgatc gatgaattga tagtgcatcc
     5941 tgtccgtcag gtccatcccc gcatggtccc gccgcctgcg cgggcctgcc ggtccacctg
     6001 agatcctccg ccggtttcac gcggtgaaga gcaccgcagc catggcgtag tcgcccgcgt
     6061 gcgagaggga gaccgcacac cggtgcaggc ctttggcttc gacgagtctg gcaacctccc
     6121 cacgcagaac aaggcgcggc tcccccaagg cgccgtggac ggtttccacg tcccgccagg
     6181 acatcctgcc cgtcttaccc gttcccagcc ccttgaccgc cgcctccttg gccgcgtacc
     6241 tggccgccag gtgcatgaac gggtccttct ggcgcctgca gtaccgcatt tcctcggggg
     6301 tgaacaccga ctccaaaagc gcctcgcgcc cctgaaagac ctcccgcacc cgcgaaacgg
     6361 cgattaggtc caccccgatg cacggctctg cggccgccga ggtttcccgg accgccgctt
     6421 tgcgcccgcg tccagcaaga ggtcgcggaa tgggaatccg gacaggcaac agggcaacca
     6481 tgctaccctg cacgtgggga tccgctgcct catcccgaaa tgttgatgcg ccaacccgag
     6541 aagccatagt ccctgttgtt aggatcgaag tctcgacccg tgaagccagg atttacctgg
     6601 agggcggtgt ggatgtactc ctccaccatt tggtcgtccc agtcgtacac cgccactcgg
     6661 atcgggaggt gattctcctc atcgaaggac agaatgaccc gatagcagta gtaagccagc
     6721 ttcggatctc gcggcagcat gccttccagg gtgtgcaccc gacggcccgc gagctctgcg
     6781 atcccccggt cggtcacctg caagaccccg ctctttgtcc ctcgccgcgc attctcaccc
     6841 acgatctcca ggagtctccc aatcccgaca tccgtcacgg gatggcgact ttgctccatg
     6901 atgcgctggt ccgagggatc cagcgcagcc gtgaacaggc ggcggatccc tcggggttca
     6961 tacagcagaa acttcccctc ccgggccccc gacacgtaga ggccttctcg acccttggat
     7021 ggtccttcca gccatttcat atagaccttg aaggggcgct ggaacttgag caggatcgtc
     7081 tcttgggggc gcaagatccc cccgattcgc tcacgaccca acatgatcga ggtatagtcc
     7141 tgcacactgg cgtagctttt ctctgccagg tccagcaggc gaagcaagtc
//
LOCUS       DBNGZC010000537         6117 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1117769, whole genome shotgun sequence.
ACCESSION   DBNGZC010000537 DBNGZC010000000
VERSION     DBNGZC010000537.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6117)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1117769"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="AC16G0_09370"
     CDS             <1..611
                     /locus_tag="AC16G0_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR5099858.1"
                     /translation="PHLVYLPERPFAMARFLGDVKAAYDQHGRCVVAVSEGIQDEKGT
                     PILTKYQETQEKDAHGNVQLSGTGALGDLLAESVRKNLNIKRVRADTFGYLQRSFFGC
                     VSDVDQHEAREVGEKSAQFAIWHNLDGSITIHRTGEYSVDYRLTPLPEVAGKTRHMPD
                     HFINEAGNNVTDAFRYYVRPLLGSGLQSAQRLRAPKVAKILS"
     gene            648..1013
                     /locus_tag="AC16G0_09375"
     CDS             648..1013
                     /locus_tag="AC16G0_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5099859.1"
                     /translation="MVGVIAAVDDLFFGAKIQETALRLNVPLRLVRSPEDLAAQARAS
                     RPALVIFDLNATACRPLEAIRRLKADPDLGSIPTLGFLSHVQQELKLAAAEAGCDRIV
                     ARSAFTSELPEILRRHAAG"
     gene            1127..2521
                     /locus_tag="AC16G0_09380"
     CDS             1127..2521
                     /locus_tag="AC16G0_09380"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR5099860.1"
                     /translation="MAKRARLLIVDEDTGSRDLFKEILEADGFTVIPAENGDVAMTCL
                     KREPVDLVFTDYVLPGMNGLALLERIRALSKPPGVIFVTAYGTIDSAVEAIKRGASGY
                     ITKPLTRERLLHLTHKAVEEIRLRQENEDLRRELDRRYTYQDLIGKSPRMQEVFQLLE
                     SLSGTESNILIQGKSGTGKELVARAVHSQSPRSDRRFVALNCGGLTETLLESELFGHV
                     KGAFTGALSHKMGVFQEADGGTLFLDEISNMPPPMQVKLLRTIQEGEVKPVGSTQTSK
                     VNVRFIAATNRDLMHDVAEGVFREDLYYRLNVITITLPDLTDRAEDIPLLASHFLERF
                     VKKLRKPVEHISEEAMGLLMDYPWPGNVRELENCVERAVVLAKGSTITPTELPPSIRA
                     ISPKEGLRLPAGMNLADLEREAILRTLSNCGGNRSAAAKTLGLSERTLYRKLRLFSAE
                     QPSIAKRARSNATV"
     gene            complement(2648..3151)
                     /locus_tag="AC16G0_09385"
     CDS             complement(2648..3151)
                     /locus_tag="AC16G0_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099861.1"
                     /translation="MRIQGWARLVACTPLVLGFLCGASCASRPPVEAKLPLGIQVENP
                     GHLRVAVAGATKRPLAAAELRPFERAGSRWDYSVQFTDTAGVGVQFLMVEATVQSLAG
                     TTATTVTRLPSRVEPMGTTPIQIHAVLSSSNPDEPENLTGVEELTFLGTDDRGDPVRV
                     IVRVPLE"
     gene            complement(3168..4241)
                     /locus_tag="AC16G0_09390"
     CDS             complement(3168..4241)
                     /locus_tag="AC16G0_09390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014653.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HIR5099862.1"
                     /translation="MPTLQPGPLESLVRDVFQAIQVPPAGAAWMAQLLVRANLRGHDS
                     HGVIRVPQYVASWKKGEANPTAEPVVVQDGPATALVDGKLGFGQIVARRGMELAMEKA
                     AAIGISAVGVFNSNHIGRLADYTEMASQRSMLALLAVNAGGAGQRMAPWGGRAPRLST
                     NPIAFACPTGKGAPISFDVATTVAAEGKVRVKRNRKEQMPSGWCLDANGSPTTDPNAL
                     YGNPPGTILPVGGHKGYSLALMIEVLAGVVARGGYSRENPGPVRNGMFMVVVDIRRFL
                     DPGTFRAEVDDLIRYLKTSPTVSGADQILTPGEPEAITEARRQQSGIFVEDQTWEQIV
                     QVSRELGVPIPRPEMTVSIPLPS"
     gene            complement(4274..4546)
                     /locus_tag="AC16G0_09395"
     CDS             complement(4274..4546)
                     /locus_tag="AC16G0_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017740867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 domain-containing protein"
                     /protein_id="HIR5099863.1"
                     /translation="MLFMLIERFKNRDAVAVYRRFREQGRMAPEGLTYVGSWIEANFD
                     RCFQLMECDDARLLQQWMLRWQDLVEFEIIPVVPSKETVDTITPML"
     gene            complement(4661..5620)
                     /locus_tag="AC16G0_09400"
     CDS             complement(4661..5620)
                     /locus_tag="AC16G0_09400"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011286609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HIR5099864.1"
                     /translation="MPTYTLDFERPILELQAKIQELRQVAQTGQVDFSSEIVRLERKV
                     ARLQAEVFANLTAWQRTQIARHPQRPYAMDYIRLMLTDFLELHGDRGFADDRAVVGGF
                     ARLEGMPLVVVGQQKGRDTKEKITRNFAMANPEGYRKALRLLRLAAKFGKPVLVLVDT
                     PGAFPGIGAEERGQAEAIARNLREMAELPVPIVVVVVGEGGSGGALGIAVGDVVLMLE
                     YAIYSVISPEGCASILWRDPGKAPDAAEAMRITAESLLQLGLIDGVVPEPTGGAHRNH
                     PETAATLKDALLKAFARLGQVPVEELLEGRYRKFRRMGVFEEG"
     gene            complement(5710..>6117)
                     /gene="dnaE"
                     /locus_tag="AC16G0_09405"
     CDS             complement(5710..>6117)
                     /gene="dnaE"
                     /locus_tag="AC16G0_09405"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes DNA-template-directed extension of the
                     3'-end of a DNA strand by one nucleotide at a time.
                     Proposed to be responsible for the synthesis of the
                     lagging strand. In the low GC gram positive bacteria this
                     enzyme is less processive and more error prone than its
                     counterpart in other bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR5099865.1"
                     /translation="LAGSVEAVIFPDVYRSSMLHLAKDSAVLVKGQVDISEESVKLLV
                     SEVRPLSLPSNGSTPLVEVTLAGAAASPDGLRRLQAILQAHPGDAPLRLHLRLPEGER
                     VTIAPEPSLGVAADEGLRQALTSAFGAGCLTMG"
BASE COUNT         1054 a         2066 c         2005 g          992 t
ORIGIN      
        1 gaccgcatct ggtttacctg cccgagcggc ccttcgccat ggcgcgattc ctgggcgatg
       61 tcaaggcggc ctacgatcag cacgggcgct gcgtggtggc ggtgtcggag ggcatccagg
      121 acgagaaagg gacccccatc ctcacgaagt atcaggagac gcaggagaag gacgcccacg
      181 gcaacgtgca gctctcgggg acgggcgccc tgggagacct gcttgccgag tccgtgagga
      241 agaatctcaa catcaagcga gtccgggcgg acaccttcgg atacctgcag cggagtttct
      301 tcggttgcgt ctcggatgtg gatcagcacg aggcccggga ggtcggcgag aagtccgccc
      361 agttcgccat ctggcacaat ctggacggtt ccatcacgat tcaccggacg ggtgagtact
      421 cggtggatta ccggctcacg cccctgccgg aagtcgcggg gaagacccgc cacatgccgg
      481 accacttcat caacgaggcc ggaaacaacg tgaccgacgc cttccggtac tacgtccggc
      541 ctctgctcgg atccgggctg cagtccgccc agaggctccg ggcgcccaag gttgccaaga
      601 tcttgagctg aaatgctggg ggaatctggc gagtcgcagg gggggttatg gtcggggtga
      661 ttgccgccgt ggacgacctg ttcttcgggg cgaagatcca ggagacggcg ctgcgcctga
      721 atgtcccgct tcgcctggtc cggtcgccgg aggatctggc cgcgcaagct cgggccagcc
      781 gccccgcgct cgtcatcttc gacctgaacg ccacggcctg ccgtccattg gaggcgatcc
      841 gccggttgaa ggccgacccg gacctgggga gcatcccgac cctcgggttt ctctcccacg
      901 tgcagcaaga actcaagctg gctgcggctg aggccggatg cgatcggatc gtcgctcgct
      961 cagcctttac ttcggaactc ccggagatcc tccggcggca cgcggccgga tgaggccgcc
     1021 cggttcggct gggggttggg ggtttcgtcg ttgcatacgt cttgtctttc gtcgggaact
     1081 caggatatac tgcatcggct cgtccggcgc agagtgaggc ggttgcatgg cgaaacgcgc
     1141 acgcctgttg atcgtggatg aggacaccgg ctcgcgggac ctcttcaaag agatcctgga
     1201 ggccgatggc ttcaccgtca tccccgccga aaacggggac gtcgccatga cgtgcctgaa
     1261 gcgggagccc gtggatctcg tcttcacgga ttacgttctg cccggcatga acggcctcgc
     1321 cctgttggag cgcatccggg cgctctccaa gccaccgggg gtcatcttcg tcaccgccta
     1381 cggaaccatc gattctgccg tcgaggccat caagcggggt gcatccggct acatcaccaa
     1441 gcccctcacc cgggaacgac tactccatct cacccacaag gccgtggaag agatccggct
     1501 gcgccaggaa aacgaggacc tgcgacgcga gctggatagg cgctatacct atcaggacct
     1561 tatcgggaag agtccgcgca tgcaggaggt cttccaactc ctggagagcc tgtcgggcac
     1621 cgaaagcaac atcctgattc aagggaagag cgggaccggc aaggaactcg tggcgcgggc
     1681 cgttcactcc cagagcccga ggagcgaccg ccggttcgtc gccctgaact gcgggggcct
     1741 caccgagacc ctcctggaaa gcgagctgtt cggccacgtc aagggagcgt tcaccggcgc
     1801 cctgagtcac aagatggggg tcttccagga ggcagacggg gggaccttgt tcctggatga
     1861 aatctcaaac atgcctccgc ccatgcaggt aaagctcctc cggacaatcc aggagggcga
     1921 ggtcaagccg gtcggctcca cccagacgtc caaggtcaac gtccgattca tcgcggcaac
     1981 caaccgggac ctcatgcacg atgtggcgga gggggtattc cgggaggacc tgtactaccg
     2041 cctgaatgtc atcaccatca cgctgccgga cctcacggac cgggccgagg acattcccct
     2101 gctggccagc cacttcctcg agcgtttcgt caagaagctg cggaagcccg tggagcacat
     2161 cagcgaagag gccatgggcc tcctgatgga ttacccctgg ccgggcaacg tgcgggagct
     2221 ggagaattgc gtcgagcggg ccgtggtgtt ggccaaggga tcgaccatca cgccgaccga
     2281 gctgccgccc tccattcgcg ccatctcgcc caaggagggc ctccgcctgc cggcgggcat
     2341 gaatctggcg gatctggagc gggaggccat cctgcgcacg ctctccaact gcggggggaa
     2401 ccgttccgcg gcggcgaaga cgttgggctt gtccgagcgc accctctacc ggaaactgcg
     2461 cctcttttcc gccgagcagc cctcaatcgc caaaagggcg cgaagcaacg caactgtctg
     2521 agacgctcct ctcacccggt tcccgaccca ccacgaggca ccagcccaca gccggaagcg
     2581 ccctacctgg ccggcggccc acatgagcca gagattgcct ggtgtcttgc tggcgggctg
     2641 tctcgcccta ttccaggggc acgcggacga tcacgcgcac gggatcaccg cgatcatccg
     2701 tgccgaggaa ggtgagctct tcgaccccgg tcagattctc gggctcgtcc ggattggagc
     2761 tggagagcac cgcgtggatc tggatgggtg tggtccccat gggctccacg cggctcggca
     2821 accgagtcac ggtcgtggcc gtggtcccgg caaggctttg gacggtagct tcgaccatca
     2881 ggaactggac gcctacgccg gcggtgtccg tgaactgcac gctgtagtcc cagcgcgacc
     2941 ccgcacgctc gaacggtcgc agctcggcgg ccgccagcgg ccgcttggtc gcgcccgcca
     3001 cggctacccg aaggtggcca ggattctcca cctggatccc cagggggagc ttggcctcca
     3061 ccgggggccg ggacgcgcag ctcgccccgc acaagaaccc gaggaccagc ggggtgcagg
     3121 ctaccaggcg agcccagccc tgaatcctca ccgcctctcc catcctgcta cgagggcaag
     3181 ggaatgctca cggtcatctc aggccggggg atcggcaccc cgagttcccg cgaaacctgc
     3241 acgatctgct cccaggtctg atcctccaca aagatgccgg actgttgacg cctcgcctcg
     3301 gtgatcgcct caggctcgcc cggggtgagg atctgatccg cacccgacac cgtcgggctg
     3361 gtcttcaggt atcggatcag atcgtccacc tcggcccgga acgtgccggg atcgaggaat
     3421 cgccggatat ccacgacgac catgaacatc ccgttccgga cggggcccgg gttctccctg
     3481 gaatacccgc cgcgggccac aacgcccgcc aggacttcga tcatgagcgc cagggagtag
     3541 cccttgtgcc cgccgaccgg caggatagtc ccgggcggat tcccgtagag ggcgttgggg
     3601 tcggtcgtgg ggctcccgtt ggcgtccagg caccagccgg aaggcatctg ctcttttcgg
     3661 tttcgcttca cccggacctt cccctccgca gccaccgtgg tggccacgtc gaagctgatg
     3721 ggggcacctt ttcccgtggg acaggcgaag gcgatagggt tggtgctgag gcgcggcgcc
     3781 cggccgcccc agggggccat ccgctgcccc gctcccccgg cattcacggc gagcagcgcc
     3841 agcatgctcc gctggctggc catctcggtg tagtccgcca gccggccgat gtggttgctg
     3901 ttgaagacgc cgacggcgga gatgccgatc gccgccgcct tctccatcgc cagctccatc
     3961 ccgcgccggg cgacgatctg gccgaacccc agcttgccgt ccacgagggc cgtggccggc
     4021 ccgtcctgga ccaccaccgg ctcggccgtg ggattggcct ctcccttctt ccaggaggcg
     4081 acgtactggg gaacgcggat gacgccgtgg gagtcgtgtc cgcggagatt ggcccggacg
     4141 aggagctggg ccatccacgc cgcccccgcg ggcggcacct ggatggcctg gaagacgtcc
     4201 cggaccaggc tctcgagcgg tccgggctga agggtgggca tggttcaaac ctcctttacg
     4261 accgacggag ggctcacagc atgggggtga tggtgtccac ggtttccttg gaagggacga
     4321 cggggatgat ctcgaactcg accaggtctt gccaccggag catccactgt tggagcagac
     4381 gcgcgtcatc gcactccatg agctggaagc agcgatcgaa attcgcctcg atccagctcc
     4441 ccacgtaggt cagaccctcg ggcgccatgc gcccctgctc acggaaccga cggtacaccg
     4501 ccacagcgtc ccgattcttg aatcgctcga tcagcatgaa cagcatgggt cctccgtcgc
     4561 agggaagccc ggtgtcgcgc tcgaaaggaa tccgccaccc gtcacacgaa gggcgccgcg
     4621 gcaactgtcc gggcgccctg agactgcgga ggttgcgcgg ctaaccttcc tcgaagaccc
     4681 ccatccggcg gaattttcga tagcgccctt ccagcaattc ctccaccgga acctgcccta
     4741 ggcgggcgaa ggccttgagc aaggcgtctt tcaaggtggc ggcggtctcc ggatgattcc
     4801 gatgggcgcc gcccgtgggc tcgggcacga cgccgtcgat cagtccgagc tgcaagagcg
     4861 actccgccgt gatccgcatg gcctcggcgg cgtccggcgc cttgcccgga tcccgccaga
     4921 ggatggaggc gcaaccttcg ggagagatca cggagtagat ggcgtactcc agcatcagga
     4981 cgacgtcccc gacggcgatg cccaaggcgc cgcccgagcc accctcgccc acgaccacga
     5041 cgacgatggg caccgggagc tccgccatct cccggaggtt ccgggcgatg gcctcagcct
     5101 ggccccgctc ttcggcgccg atcccgggaa acgcccccgg ggtgtccacc agcacgagaa
     5161 cgggcttccc gaatttcgcc gccagccgca ggaggcggag ggccttgcgg tacccttccg
     5221 ggttggccat ggcgaaattg cgcgtgatct tctccttggt gtcccggccc ttctgctgtc
     5281 cgacgacgac cagcggcatg ccttccagcc gggcaaagcc tcccacgacc gcccgatcgt
     5341 cggcgaagcc gcggtccccg tgcaactcca ggaagtccgt cagcatcagc cggatgtagt
     5401 ccatggcgta ggggcgttgc ggatgacggg cgatctgggt ccgctgccag gcggtgaggt
     5461 tggcgaagac ctctgcctgc agccgggcga ccttccgctc cagacgcacg atctcgctgg
     5521 agaagtccac ctggcccgtc tgggcaacct gtcgaagctc ctggatcttg gcctgcagct
     5581 ccaggatcgg gcgctcgaaa tccagggtgt aggtcggcat gcgaacccct cagagaggcc
     5641 gacagggaca cagacaggga gaaagcgaac cgacggggaa gattcttccc gcgtccctgt
     5701 cgagcattcc tatcccatcg tcaggcagcc ggcgccgaac gccgacgtga gcgcctgacg
     5761 caggccttcg tccgccgcca cgcccagcga gggttccggg gcgatggtga cccgctcgcc
     5821 ctcgggaagc cggaggtgca gtcgtagcgg tgcgtccccg ggatgcgcct gaaggatggc
     5881 ctgcaggcgc cggagcccgt cggggctggc cgccgcgccg gccagggtca cttccaccaa
     5941 gggggtgctc ccgttgctcg gcagggagag cgggcggacc tcgctgacca ggagtttgac
     6001 ggactcctcc gagatgtcca cctggccctt caccagcacc gcggaatcct tggcgaggtg
     6061 gagcatgctg gaccggtaca cgtcggggaa gatcaccgcc tccaccgagc ccgccag
//
LOCUS       DBNGZC010000538         2118 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2590380, whole genome shotgun sequence.
ACCESSION   DBNGZC010000538 DBNGZC010000000
VERSION     DBNGZC010000538.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2590380"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="AC16G0_09410"
     CDS             complement(<1..805)
                     /locus_tag="AC16G0_09410"
                     /inference="COORDINATES: protein motif:HMM:NF013460.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HIR5099866.1"
                     /translation="MNQHRDLEPGAPEVEALLRDSLKQELEGEIRQALEAELREELSQ
                     ELRERVVTRLKTELAEEIRVRIARVKAELEAEILAKVVPAPAPREFERFSWNFRAQHL
                     VLMASCLILILTGLPLKFHEARVSQLFFDLVGGVQTSTLIHRVGAVGLICVGAYHLFY
                     LVVFREGRRNFLELLPGPKDALDLLRMLRYFLGRTEEKPRFGRFSYVEKFDYWAVYWG
                     IVVMIGSGLILWFLETSLQFLPKFAADIAREAHSDEGLLATLAIIIWHFY"
     gene            complement(802..1974)
                     /locus_tag="AC16G0_09415"
     CDS             complement(802..1974)
                     /locus_tag="AC16G0_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR5099867.1"
                     /translation="MRWSELNFRRADQAVGERGTSRPMQGGSSPKERPGCISVSGWSW
                     LLVALSGLILVLFATGKGEALTKCLICHGKPNFSKALPSGRSVSLFVDEKQLKESVHA
                     GRSCTDCHSDITAIPHKGEIKRVGCVRCHFKGNPVGAPQKDIYVEYSKSVHGIAAAAG
                     NVKAPVCQDCHGTHNIHRPKDPASQMNKAHIPETCGRCHLVVFSDYRESAHWKAFLEG
                     KRDSAVCTDCHGEHTIRAHENPDSSTYVTRVPETCSRCHASVAVVGKYGIKTEQVATY
                     GRSYHGVAIKFGGKTVANCASCHGVHDIRPSNDPKSAGYIVNIPKTCGKCHTGANANY
                     AKGKIHVDPTKKEAGLIYWVSLFFKWLTISTMVGLIGHIGLDLFHRVRRRGQAHAP"
BASE COUNT          379 a          686 c          663 g          390 t
ORIGIN      
        1 tgtaaaagtg ccagatgatg atggccaggg tggccagcag cccctcgtcg ctgtgggctt
       61 cgcgggcgat gtctgcggcg aacttgggca ggaactggag ggaagtctcc aggaaccaca
      121 ggatcaggcc ggagccgatc atgacgacga tgccccaata caccgcccag tagtcaaact
      181 tttccacgta gctgaagcgg ccgaagcgcg gcttctcctc ggtccggccc aggaagtaac
      241 ggagcatccg caaaaggtcg agggcatcct tcgggcccgg caagagttcc agaaagttcc
      301 ggcgcccttc ccggaagacc acgaggtaga agaggtggta ggctcccacg cagatgagac
      361 ccaccgcgcc gacccggtgg atgagggtgc tggtttgcac cccgccgacg agatcgaaga
      421 agagttggga tacccgcgcc tcatggaatt tcagcggaag cccggtgagg atgaggatga
      481 ggcagctcgc catcagcacc aagtgctgcg cccggaagtt ccagctgaac cgctcgaact
      541 cacggggggc gggggcgggc acaaccttcg cgaggatctc cgcctccagc tccgccttga
      601 cgcgggcgat gcgaacccgg atctcctccg ccagctccgt cttcaggcgg gtcaccacgc
      661 gctcgcgaag ctcctgactc agctcctccc gcagctccgc ctcgagggcc tgccggatct
      721 cgccctccag ttcttgcttc agcgagtcgc ggaggagggc ctcgacctcc ggagcacctg
      781 gctcgagatc ccgatgttga ttcatggcgc gtgtgcctgc ccgcgtcgtc ggacgcgatg
      841 gaagaggtcc aagccgatgt gaccgatcaa acccaccatg gtcgagatcg tcagccattt
      901 gaagaagagg ctgacccagt agatcaatcc cgcctccttc ttggtgggat ccacgtggat
      961 cttgcccttg gcgtagttcg cgttggcccc ggtgtgacac ttcccgcacg tcttaggaat
     1021 gttgacgatg taccccgccg atttggggtc gttcgaaggt cgaatgtcgt gcaccccgtg
     1081 gcagctggcg cagttggcca cggtcttccc gccgaacttg atggcgacgc cgtggtagct
     1141 ccgcccgtag gtggccacct gctccgtctt gatcccgtac ttcccgacca cggcgacgga
     1201 tgcgtggcac cgggagcagg tttcgggcac ccgggtcacg taggtggagg aatccggatt
     1261 ctcgtgagca cggatcgtgt gttccccgtg gcagtccgta cacacggccg agtcccgctt
     1321 tccctccagg aacgctttcc agtgggcgct ctcgcggtag tcgctgaaga ccaccaggtg
     1381 gcaccggccg cacgtctcgg ggatgtgcgc cttgttcatc tgggaggcgg ggtccttggg
     1441 ccgatggatg ttgtgggtgc cgtggcagtc ctggcagacc ggggccttca cgtttcccgc
     1501 cgcagcggcg ataccgtgca cgctcttgga gtattccacg tagatgtctt tctggggggc
     1561 gcccaccgga ttccccttga agtgacagcg gacacatccc actcgcttga tctcgccctt
     1621 gtgggggatg gccgtgatgt cgctgtgaca gtcggtacag gaccgcccgg catggaccga
     1681 ttccttcaac tgtttctcat ccacgaacag ggaaactgat cggccgctgg gcagggcctt
     1741 cgagaagttg ggcttcccgt ggcagatcaa gcacttcgtg agagcctcgc ctttccccgt
     1801 cgcaaagagc acgaggatca acccgctcag ggcgaccagg agccacgacc acccggaaac
     1861 cgagatgcat ccgggtcgct ccttcgggga ggacccgccc tgcatcgggc gcgacgtccc
     1921 ccgctctccc acggcctgat ctgccctccg aaagttcagc tcactccatc gcatcgatgc
     1981 gccccgcccg gcaaagccga tcgtactcca cccggtgccg ctccttgagg tcacccacgc
     2041 tgatccgacc ggtgatccag gaccagtcca tgggaaacac ctcaggccgg aggtggacat
     2101 cgtacaggtg ccatacga
//
LOCUS       DBNGZC010000539         1992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2001413, whole genome shotgun sequence.
ACCESSION   DBNGZC010000539 DBNGZC010000000
VERSION     DBNGZC010000539.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2001413"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="AC16G0_09420"
     CDS             <1..1001
                     /locus_tag="AC16G0_09420"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018221868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HIR5099868.1"
                     /translation="LAAARRGDQEAFARLIEPYQVELLAHCYRILGALEDAEDGLQET
                     FLRAWRRLETFEGRSSLRAWLYKVATNVCFDLLDARRGSRRLPNSISPPGNPNHALPP
                     ASPEALWVGPFPDAWIDQAPDVYPEARIELRESITLAFIAALQTLPGRQRAVLLLRDV
                     MGWNAAETAQTLDMSTAAVNSALQRARDTLKHSSKRPDRPGPGRLQEPLSSLLGRYVA
                     AWEAADSAALVATLREDVKLTMPPLPLWFEGRRDIRAFLDGTLFRSLAPFRVRLVPAR
                     LNAAPSFAVYQMDASGIFRGVALHVLTLENGAISEIHDFLALDGPLFSSFGLPATV"
     gene            1086..1763
                     /locus_tag="AC16G0_09425"
     CDS             1086..1763
                     /locus_tag="AC16G0_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099869.1"
                     /translation="MDPNLNLYLLAIRGTLASSTLDAARKIHNSTAGAPENIAAVRSL
                     GDMSHMVYVPVEPPGSGAGEFLIMDIWTSPDGINQFFSNPTVQEQAGQIFSQRDPVVW
                     RAAEGFTSYHIPAPFGKNDRILTTARGMLKSVEAGCGLHNASIAKMIGKARKAGNLSH
                     EAYLRLAAPGSPEAQEFFAVDVWMSAEGMMEVYDDPEFLEGFDHMFAAEATTAVWTHP
                     TGNWVEW"
BASE COUNT          332 a          659 c          616 g          385 t
ORIGIN      
        1 gccttgccgc tgcacgccgc ggcgaccagg aggcttttgc gcgcctgatc gagccctacc
       61 aggtggaatt gctcgcccat tgttaccgca tcctgggcgc actggaggac gccgaggatg
      121 gcctccagga aacgttcttg cgcgcctggc gccgcctgga gacgtttgag ggacgctcgt
      181 cccttcgtgc ctggctctac aaggtcgcca cgaatgtgtg cttcgacctg ctggacgcgc
      241 gccgcggcag ccgccgcctc cccaacagca tctcgccgcc cggcaacccg aatcatgcct
      301 tgccgcctgc ctccccggag gccctgtggg tagggccgtt ccccgatgcg tggatcgacc
      361 aggcgccgga cgtttatccg gaagcacgca tcgagctgcg agagagcatt accctggcct
      421 tcatcgccgc gttgcagacg cttcccggcc ggcagcgtgc cgtcctgctc ctccgcgatg
      481 tgatgggctg gaatgctgcc gagactgccc agacgctgga catgagtacg gccgcggtca
      541 acagcgccct ccagcgcgcc cgggacacgt tgaagcattc ctcaaaacgg ccggaccgcc
      601 cgggtcccgg caggctgcag gagccgcttt catccctcct ggggcgctac gtcgcagcct
      661 gggaggccgc cgattccgcc gccctcgtcg ccacgctgcg ggaagatgtg aaactcacca
      721 tgccgcctct cccgctttgg tttgagggcc ggcgggatat ccgggccttc ctcgatggca
      781 ccctgtttcg aagtctcgcc ccgttccggg tgcgcctcgt cccggcgcgc ctcaacgccg
      841 cgccttcttt tgcggtttat cagatggacg cttccggcat ctttcgcggc gtggccttgc
      901 acgtcctgac cctcgagaac ggagcgatct ccgagatcca cgatttcctg gcccttgacg
      961 gtccactttt ctcctccttt ggacttcctg ccacggtctg accgatgatt ttggtcgaat
     1021 cctgccgtct cattagtgag agttgcagga aagatcgagt ctgtccaagc caaaaggaga
     1081 aaagaatgga tccgaatctt aatttatacc ttctcgcgat ccgcgggact ctggcatcct
     1141 ccacactgga tgccgcgcgc aagatccata attccacggc gggcgccccg gagaacatcg
     1201 ccgccgtgag gtccttgggg gacatgagcc acatggtgta tgtgcccgtg gagcctcccg
     1261 ggtccggcgc gggggaattc ctgatcatgg atatttggac gagcccggac gggatcaacc
     1321 agttcttctc gaacccgacg gtgcaggagc aagccggaca gatattcagc cagcgcgatc
     1381 cggtggtctg gcgggcggcc gagggcttca ccagttacca catccctgcc ccgttcggaa
     1441 agaacgaccg catcctcacc actgctcgcg gcatgctcaa gtccgtcgag gcgggctgcg
     1501 ggctgcataa tgcctccatc gccaagatga tcggcaaggc gcgcaaagcc gggaaccttt
     1561 cgcacgaggc ctatctccgg cttgccgctc cgggctcacc cgaagctcag gagtttttcg
     1621 cggtggacgt ctggatgagc gccgaaggca tgatggaagt ctatgacgac cccgaattcc
     1681 tggagggctt cgaccacatg ttcgcggccg aggcgaccac ggccgtgtgg acccacccga
     1741 caggcaactg ggtggagtgg taaggggatc gcgagtccgc ctcacgagag ggcgaccgac
     1801 ctggccgccc tcgttttgtg ttgacaccag gtagaggtac ggcgggatcc gtacgggcac
     1861 ggcgaggagt ctgaaagtta ttgtccaccg accgtctcgc cgtgcccctg cacgtctggc
     1921 tgacgccggc acctgcgtgc tcggggcagg tgtagctgac agctgatcgc tgacggctga
     1981 atttgtcttt cc
//
LOCUS       DBNGZC010000540         4197 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1117866, whole genome shotgun sequence.
ACCESSION   DBNGZC010000540 DBNGZC010000000
VERSION     DBNGZC010000540.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1117866"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="AC16G0_09430"
     CDS             <1..1098
                     /locus_tag="AC16G0_09430"
                     /inference="COORDINATES: protein motif:HMM:NF013717.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HIR5099870.1"
                     /translation="PILDMLWDYQHNPSDRKVEPKMELVLKEVNGYATADILDKDGKV
                     VLKKGQPVPGFAALRDDGLTACGNWIYSGVYPAEGKNLAARREKAASPADYMNHNWGF
                     AWPANRRILYNRASADPSGKPWSERKKLIWWDVDQKKWVGGDVPDFKADLPPDSPKLK
                     EGPFRGLDGQDVFIMRADGKGAFFGPLLDGPFPEHYEPFESPTPNLLSKVDNNPVAKV
                     WKIGDLNKLGKPDEYPIVLTTYRLTEHHAAGMSRHVPWLAEKFAGHFAEISHDLAADR
                     GIANGDMITVVTPRGKIHLRALVTERFRPFMIDGKKVHHIGIPWHWGWLGVMPSAVGD
                     VTNDLVASVGDPTTFIQESKALLCDVKKGEV"
     gene            1101..1889
                     /locus_tag="AC16G0_09435"
     CDS             1101..1889
                     /locus_tag="AC16G0_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006139281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5099871.1"
                     /translation="MARPLAMFTDTSVCIGCRACEVACKQWNQLPAETPAWTGSYAAQ
                     PHFTDKTWRHVRFIEKKTEGGDPKWLLMSDVCKHCNEAGCLEACPTGAIYRTAFGTVD
                     INQDICNGCRYCVSACPFGVVHFNEGTGRVNKCTFCVDRTSEGMQTACAKTCPTQSIQ
                     FGHRDELAVKAYKRLDELKAQGVKEANLYGVDEQGPLGGLNAFFLLLDKPEVYGLPEE
                     PRLPQKPIFEDAAISAGTAILAGIGAVVAFRERGDGGGGEGGEE"
     gene            1892..2827
                     /gene="nrfD"
                     /locus_tag="AC16G0_09440"
     CDS             1892..2827
                     /gene="nrfD"
                     /locus_tag="AC16G0_09440"
                     /inference="COORDINATES: protein motif:HMM:NF015851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR5099872.1"
                     /translation="MPNLTSPHWPPIIEVYFFLVGLAGGAFMAAGAADIFGNKQDRVV
                     TKVGSWLALLALIPGPIFLIIDLGMPSRFLHMLFVSKASTNIGLSAITIGPFHIKPYS
                     PMNLGAWALMGFGICAAGVVATLYLEDSRRMQGMVPLRGAFGAIGVLLGFFVAGYPGQ
                     LLAVTAQPFWTNARPLGALFMAIGAATGLAAIALILSLGGAEVGSSLVKVRRAYTFCV
                     ILQALALMAVLWAVAQGPAWSAGRIWLLLGGRYSPVFWIGAVLVGVLAPLVLEYRDGF
                     FQGYRQGPGRVALTSALILVGGFLMKYVIFAVGQA"
     gene            2895..3170
                     /locus_tag="AC16G0_09445"
     CDS             2895..3170
                     /locus_tag="AC16G0_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099873.1"
                     /translation="MHTSHHVMGMVTTVDLEAGHLRLKDESESFCTVCCGGETEILGE
                     EDPLALEGLQPGDYIRSECLRGEDGKLVAKKIVLLRPAWRMIESPEM"
     gene            3204..4100
                     /locus_tag="AC16G0_09450"
     CDS             3204..4100
                     /locus_tag="AC16G0_09450"
                     /inference="COORDINATES: protein motif:HMM:NF051207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HIR5099874.1"
                     /translation="MAEWEWPATHLLNEGTSMLQDRLWEEIATTFEKARRRQPALGEF
                     LAFFEKLVKEQHSATVNRGVFTFDEAKLRGRSTRSEPVLPRAEFPLDLACAETLFRTL
                     CTMAKERGPEVRSAAQEIEASIGWGGMELPDLLRGTMAEEGYAYAVADQRSLDPELLH
                     MLAEASILPSLQATAKSVSAFVENDRWLKGVCPVCGSEPKFAELRGQELAASRYLHCG
                     FCVWAWPARRLGCPFCDNKDHEHLQTLIIENHLSCKLEACDVCHRYLKLVDNKEFIGL
                     IPEVEVLVTPHLDLAVMERGYH"
BASE COUNT          759 a         1237 c         1436 g          765 t
ORIGIN      
        1 cctatcctgg acatgctctg ggactaccag cacaacccca gcgaccggaa ggtcgagccc
       61 aagatggagc tggtgctgaa ggaggtcaac gggtatgcga ccgccgacat cctggacaaa
      121 gacgggaagg tggtcctcaa gaagggccag cctgttccgg gcttcgcggc gcttcgggat
      181 gatggcttga cggcgtgcgg caactggatt tactccggcg tatatcccgc ggaaggaaag
      241 aacctggcag cccggcgcga gaaggcggcg tccccggcgg attacatgaa tcacaactgg
      301 ggttttgcct ggccggccaa ccgccgcatc ctgtacaacc gggcctcggc cgacccctcg
      361 ggcaagccct ggagcgagcg gaagaaactc atctggtggg atgtggatca gaagaaatgg
      421 gtgggagggg atgtgccgga tttcaaggcc gatcttcccc cggactcgcc caagctaaaa
      481 gaaggaccct tcagggggct ggacggccag gacgtgttca tcatgcgggc ggacgggaag
      541 ggggcgttct tcggcccgct gctggatggc ccgttccccg aacactacga gccgttcgag
      601 tcccccacgc cgaatctgct ctccaaggtg gacaacaatc cggtggccaa ggtctggaag
      661 atcggggacc tgaataagct ggggaagccg gacgagtacc cgatcgtcct gacgacgtac
      721 cgtctgacgg agcaccacgc ggccggcatg tcgcggcacg tcccctggct ggcggagaag
      781 ttcgccggcc atttcgccga gattagccac gacctggcgg cggaccgagg cattgcaaac
      841 ggtgacatga tcacggtcgt gacaccgcgc ggcaagatcc acctccgcgc cctggtgacg
      901 gagcgtttcc gccctttcat gatcgacggc aagaaggtcc atcacatcgg cattccgtgg
      961 cactggggct ggctgggcgt gatgccaagc gctgtgggcg acgtgaccaa cgacctggtg
     1021 gccagcgtcg gcgatccgac cacctttatt caggagagca aggcgctcct gtgcgacgtg
     1081 aagaaggggg aggtgtaaga atggccagac cgctcgctat gttcaccgac acctcggtgt
     1141 gcatcggctg ccgcgcctgc gaggtggcgt gcaagcagtg gaaccagctt ccggcggaga
     1201 cgcctgcgtg gaccgggagt tatgcggccc agccgcattt caccgacaag acctggcggc
     1261 acgtccggtt catcgagaag aagaccgagg gcggcgatcc caagtggctg ctcatgagcg
     1321 acgtctgcaa gcactgcaac gaggccggct gcctggaagc ctgtccgacc ggcgccatct
     1381 atcgaaccgc cttcggcact gtggacatca accaggacat ctgcaatggc tgccgttatt
     1441 gcgtatcggc ctgccccttc ggcgtcgtgc acttcaacga ggggacgggg cgggtcaaca
     1501 agtgcacctt ctgcgtggat cggacctctg agggtatgca gaccgcctgc gccaagacct
     1561 gtcctacaca gtccatccag ttcggacatc gcgacgagct ggcggtcaag gcctacaagc
     1621 ggctggacga gctgaaggcc cagggtgtga aagaggccaa cctgtacggt gtggacgagc
     1681 agggtccgct gggtggtctg aatgccttct tcctccttct ggacaagccc gaggtgtacg
     1741 ggctgcccga ggagccccgg cttccccaga agccgatctt tgaagatgcg gccatcagcg
     1801 ccgggaccgc catcctggcg gggattggcg ccgtcgtggc cttccgggag cgtggcgatg
     1861 gaggcggcgg agagggaggg gaggagtagc catgccgaat ctgacgagcc cacactggcc
     1921 gcccattatc gaagtctatt tcttcctcgt cggcttggcc ggcggcgcct tcatggccgc
     1981 cggggctgcc gacatttttg gaaataagca ggatcgcgtg gtcaccaagg tggggagctg
     2041 gctggccctc ctggccttga tccctgggcc gatctttctc atcatcgact tgggcatgcc
     2101 gtcacggttt ctgcacatgc tgtttgtttc caaggccagc acgaacatcg gcctgagcgc
     2161 catcaccatc ggtccctttc acatcaagcc gtactcgccg atgaacctgg gggcatgggc
     2221 gctcatgggc ttcggcatct gtgcggcggg ggtggtggcg acgctttacc tggaggacag
     2281 tcgtcgcatg cagggcatgg tcccgctcag gggagccttc ggggcgatcg gggtcttgct
     2341 gggcttcttt gtggccgggt atcccggtca gctcctggcc gtgaccgccc agcccttctg
     2401 gaccaatgcc cgccccctgg gtgcgctctt catggccatt ggcgcggcga cgggcctggc
     2461 ggccattgcc ttgatcctga gtctgggcgg cgccgaggtc ggtagcagtc tggtgaaggt
     2521 acgtcgtgct tacacattct gcgtcattct ccaggccctt gcgctcatgg cggtcctttg
     2581 ggcggtggca cagggaccgg cctggagcgc cggccgcatc tggctgctcc ttggcggtcg
     2641 gtacagcccg gtcttctgga tcggcgcagt tcttgtcggt gtcctggcgc ccctcgtcct
     2701 ggaataccgg gacggattct tccaaggcta tcggcagggc ccagggaggg ttgccctcac
     2761 gtccgcattg atcctggtcg gcggtttcct gatgaagtac gtgatctttg ccgtgggaca
     2821 ggcctgatcc ggctccgcgc tgacacggag cagtgcacgg ggtatgtcca tgggagagaa
     2881 atcggagggg acggatgcat accagtcatc acgtcatggg gatggtgacc acggtggatc
     2941 tggaggcggg tcaccttcgg ctcaaggatg agagcgagtc cttctgcacc gtgtgttgcg
     3001 gcggcgagac agagatcttg ggagaggagg atccgctggc cctggaaggt ctccagcccg
     3061 gcgattacat ccggagtgag tgcctcaggg gagaagacgg aaagctggtg gccaaaaaga
     3121 tagtcctcct ccggccggcc tggcgcatga tcgagagccc ggagatgtga gggagggacc
     3181 tggtagcgtt cgttgccggg gtcgtggccg agtgggagtg gccggcgacc catctcttga
     3241 atgaggggac gagtatgctg caggatcgcc tgtgggaaga gatcgccacc accttcgaga
     3301 aagcacgccg acggcagccc gcactcgggg agttcctggc tttcttcgag aagctggtga
     3361 aggagcaaca ctccgcgacg gtgaatcggg gcgtcttcac cttcgatgag gccaagctgc
     3421 ggggccggag cacgcggagc gagccggtct tgccgcgggc tgaattcccc ctggacctcg
     3481 cctgcgcgga gaccctcttc cggacgctct gcacgatggc caaggagcgt ggaccggagg
     3541 tgcggtccgc cgcccaggag atcgaggcct ccatcggctg gggtgggatg gaacttccgg
     3601 acctgcttcg aggcaccatg gcggaagaag ggtacgccta tgccgtggcg gaccagcgaa
     3661 gcctcgatcc ggaactgctc cacatgctgg ccgaggcgag tatcctcccg agcctccagg
     3721 caacggccaa gagcgtcagc gctttcgtgg agaatgaccg atggctcaaa ggggtctgtc
     3781 ccgtctgcgg ctcggaaccg aagttcgcag agctgcgcgg ccaggagttg gcggcctccc
     3841 ggtatttgca ctgtggcttc tgcgtctggg catggccggc ccgccgtctt gggtgtccct
     3901 tctgcgacaa caaggaccac gagcacctgc agaccctcat catcgagaac cacttgtcgt
     3961 gcaagctgga agcgtgtgac gtgtgccatc gctacctgaa gctggtggac aacaaggagt
     4021 tcatcggcct gatcccggag gtggaagtct tggtcacacc ccacctggac ctggcggtta
     4081 tggagcgggg ctaccactaa agtggaggcg gaaccgtgtg gcggaagaaa aaggtcgatg
     4141 agtgggtcgt ggtcgggctc ttgaaccagg ccaaggacga gatcgatgac ccgaccg
//
LOCUS       DBNGZC010000541         1247 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1316910, whole genome shotgun sequence.
ACCESSION   DBNGZC010000541 DBNGZC010000000
VERSION     DBNGZC010000541.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1316910"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1065)
                     /locus_tag="AC16G0_09455"
     CDS             complement(172..1065)
                     /locus_tag="AC16G0_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5099875.1"
                     /translation="MRWATLGRGRFTFVVLALPAILYVVVWRIAPAVYTVWLSLTQYN
                     LVYDPQPQWNNLENYRRLLHDSGLVEALRLSAIFALAATVVELVLGLLAAAFFDSDPP
                     ARNLLLGVFLLPMIMAPVVVGTVWSALFDQTVGPIPYLLEVLHGPNILWLATPLSALF
                     SLVAADAWEWSPLMALLLFSAMQVIPREQYEAARVDGATVIQLFLRISFPQITGMVGV
                     AAGLRFMDAFLELDKVFVMTGGGPGTSTQFVSYYVYKQAFQSYELGYASTVITGLLGL
                     LAVVYALYLRNYGRKPKPMPA"
BASE COUNT          247 a          402 c          412 g          186 t
ORIGIN      
        1 tcgaccatga tgcgctcggg tttgacagag agcagcacca ggtagagaaa ggcggggagc
       61 gtccagccga ggatgagcgc cgtccccagc aggtgccccc accgcctgcg tcgtcgtcgg
      121 ccggtcatgt tgcctcccac tctccctccc gccagtcccg gcggccggag cctacgcggg
      181 catcggcttg ggtttccggc cgtagttccg gagatacagc gcgtagacaa ccgccagaag
      241 tcccagcagc cccgtgatca cggtcgaggc gtatcccagt tcataggact gaaacgcctg
      301 cttgtacacg tagtagctca cgaactgggt cgaggttccg gggccgccgc cggtcatcac
      361 gaagaccttg tccagctcca ggaaggcgtc catgaaccgg agccctgcgg cgacaccgac
      421 catgccggtg atctgcggga agctgatccg aaggaagagc tggatgacgg tcgccccatc
      481 gaccctggcg gcctcgtatt gctcgcgggg aatcacctgc atggccgaga acaggagcaa
      541 ggccatcagg ggggaccatt cccaggcgtc ggccgccacg aggctgaaga gagccgagag
      601 cggcgtcgcc agccacagga tgttgggccc atgcaggacc tcgaggaggt agggaatggg
      661 gcccaccgtc tgatcgaaga gcgcggacca gacggtcccc accacgacgg gcgccatgat
      721 catcggcagc aggaacaccc ccaggaggag attccgcgcg ggagggtcgc tgtcgaaaaa
      781 ggcggcggcc agcaggccca gcaccagttc cacgacggtc gcggcgagcg cgaagatggc
      841 cgagagcctc agggcctcca ccaggccgct gtcatgcaaa aggcgcctgt aattttccag
      901 gttgttccac tgcggctggg ggtcatagac caggttgtac tgcgtcagac tcaaccagac
      961 ggtgtacaca gcgggggcaa tccgccagac cacgacgtag aggatagccg gcagggcgag
     1021 caccacgaag gtgaaccggc ctcggccaag cgtcgcccat ctcatggcgc caccccgtat
     1081 gcggacggtg tcagcgagaa tgccagccaa ggcgtggtcg cggatctcgg cgcggccacg
     1141 ccctggcgaa aagctccgct tgcacggcta gcgttcgttt cgcccctatc tcacttgatg
     1201 tagcgggcgc gcttgaatac ctccacgacc ttggcctggg ccgagtt
//
LOCUS       DBNGZC010000542         5185 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_47009, whole genome shotgun sequence.
ACCESSION   DBNGZC010000542 DBNGZC010000000
VERSION     DBNGZC010000542.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5185)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5185)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5185
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_47009"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1732
                     /locus_tag="AC16G0_09460"
     CDS             <1..1732
                     /locus_tag="AC16G0_09460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5099876.1"
                     /translation="NARLLLRHVNDLLDLSKIDAGRMAPKYAEVDLAQLTRVAASNFK
                     SLAAEGGVSYVIEAPDTLRAEVDPPQLERVLLNLLSNAFKFTPAGGRIRVSARREGER
                     AILEVEDTGPGVPPHLREAIFERFRQVEGGSVRRFGGTGLGLSIAKEFVALHGGSITV
                     GDPPGGRGSVFRVEFDLCAPSGKEVRRTAWQPDEAPGRQMVEDLGLRNPARQHAGRAR
                     PGDPVVLVVEDNPDMNAFIARTLSRTYRVESAFDGQEGLEKAKEILPDLILCDVMMPR
                     MSGEQMVRELRRCRALDDVPVVLLTAKVDDEELRVALLKDGAQDYLSKPISVDHLLAK
                     VERLIADRRRAAEELHRTREVSARLLTVHDQERKRIAEELSENIAQCLAGLGMSLSLA
                     RNSDTAPSLEVQRILAEGLELLQRTSQSIRALCYVLHPTLLDHFGLAAAIEWHVDDFA
                     RRSGIDVTLDLPHGLGELPVEHELALFRVMEEALTNVRRHSGSAKATVRIFQDASEIG
                     LEVIDEGHGMLPSEASETTTQGAGIPTMRERARGLGGRLEIQSGKGGTSVRVVLPIPA
                     ETRSLVSEPV"
     gene            2207..2869
                     /locus_tag="AC16G0_09465"
     CDS             2207..2869
                     /locus_tag="AC16G0_09465"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR5099877.1"
                     /translation="MPRQEMGEHFTRCAIDLPFRYRESTEVAYRPGVGWTRDLSGGGA
                     WVELPERVAPPTELEVGLHALGEPLRLAAQVVWGCPEPHVRSRLHGLRFIRITPAQRQ
                     RLGVLITHQKSREVGRLYCSLAATCQSKAAAGVPLPCETRDLSFGGVSLRLPALAPPG
                     THLQVKILTAFGAVTADAQVVWVEPPDARPRNAPCRHGLRFLRLEPSSDLPLKALLAG
                     LR"
     gene            4166..4573
                     /locus_tag="AC16G0_09470"
     CDS             4166..4573
                     /locus_tag="AC16G0_09470"
                     /inference="COORDINATES: protein motif:HMM:NF016408.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR5099878.1"
                     /translation="MEEPVMSAIQPKGAESLVAKGLTIEGKIEGSGDVRIAGRFKGTI
                     NVKGEFRIESGATIEGEVTADTVLVGGEVNGKIVASSRVEFKESGSLIGDLKAGSLTV
                     AAGSKMRGNVEFGWKEGEVESLPLKERGHAAIR"
     gene            4586..4951
                     /locus_tag="AC16G0_09475"
     CDS             4586..4951
                     /locus_tag="AC16G0_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099879.1"
                     /translation="MTGESWKCPHCGEQILRSAVICPACQRRLHVNALATHQTTQVIA
                     SPLNVEGVIRHPGTGAAWEYSVLVEVQDQEGEVLARRVVGVGALQPGEARVVRLRVDM
                     HSREKITPAAAAARQPKSE"
BASE COUNT          942 a         1607 c         1699 g          937 t
ORIGIN      
        1 caatgcccgg ctgctcctgc ggcacgtcaa cgacctgctc gacctctcca agatcgatgc
       61 cggccgcatg gcccccaagt acgccgaggt cgacctggcc caactgacca gggtcgcggc
      121 ctcgaatttc aagtcactgg cggccgaggg tggcgtcagc tacgtgatcg aggcgccgga
      181 tacgctccgg gcggaagtcg acccgccgca actcgagcgg gtcctgctca atctcctctc
      241 caacgccttc aagttcaccc cggccggcgg ccgtatacgg gtctctgccc ggagggaagg
      301 cgagcgggcg attctcgaag tcgaagacac cggccccggg gtcccgccgc acctgcgtga
      361 ggccatcttc gagcgattcc gccaggtcga gggcgggtcg gtccggcgct tcggcggcac
      421 cgggctgggg ttgtcgatcg ccaaggagtt cgtcgccctc catggcggga gcatcaccgt
      481 cggagaccca ccgggcggaa gggggtcggt gttccgggtt gagttcgacc tctgcgcacc
      541 cagcggcaag gaggtcaggc ggacagcctg gcagccggac gaagcgcccg gccggcagat
      601 ggtcgaggat ctggggctgc gcaatccagc ccggcagcac gcgggcaggg cgcgtcccgg
      661 cgaccccgtg gtcctggttg tcgaggacaa ccccgacatg aacgcgttca ttgcgaggac
      721 gctgtcgcgg acataccgcg tggaatcggc gttcgacggc caggagggcc tcgagaaggc
      781 gaaggagatc ctccccgacc tgatcctctg cgatgtcatg atgcctcgca tgagcggcga
      841 gcagatggtg cgggagcttc gccggtgccg ggcgctggac gacgtgccgg tggtgctgct
      901 gacggccaag gtcgatgatg aggagttgcg cgtggcgctc ctgaaggacg gcgcccagga
      961 ttacctgagc aagccgatca gcgtggacca cctgctggcc aaggtcgagc ggctcatcgc
     1021 ggaccgccgg cgcgctgccg aggaattgca ccggacgcgt gaggtctcgg cacgcctcct
     1081 cacggtccac gatcaggagc gcaagcgcat cgccgaggaa ctgagcgaga acatcgccca
     1141 gtgccttgcc ggcctgggga tgagcctgtc cctggcgcga aattcggaca cggccccgag
     1201 cctggaggtg cagcgcatcc tggccgaggg actcgagttg ttgcagcgga cttcgcagag
     1261 cattcgcgcc ttgtgctacg tcttgcaccc gacgctcttg gatcacttcg gattggcggc
     1321 ggcgatcgag tggcatgtcg atgacttcgc ccggcgtagc gggatcgacg tgacgctcga
     1381 cctcccacac ggcctcgggg agctaccggt cgagcacgag ctggcattgt tccgggtcat
     1441 ggaagaggcg ctgacgaatg ttcgccgtca ctccgggagc gccaaggcca ccgtccgcat
     1501 ctttcaggac gcctccgaga tcgggctgga ggtgatcgat gaaggccacg ggatgctgcc
     1561 ctccgaggcg tccgagacca ctacccaggg tgctggcatc cccacgatgc gagagcgcgc
     1621 caggggtctc gggggacggc tggagatcca gtccggaaag ggagggacca gcgtcagggt
     1681 cgttctcccg attcccgcgg agacgagatc cctcgtctca gagcccgtgt gatccgcaag
     1741 cgcagggtcg tcacctcccg ctccacccgc gaccacgcgg gatgcgattg atgtcccacg
     1801 accacgctct cccagagcga gcggtatgcg tgggctgggt ctctctcctc cggtacgcca
     1861 attgtgcaag acaatgcttg aacctccgcc aggattgtgg acgcgagaat caagctcccg
     1921 tggaatacgc catcgatcca aggcagcttg gtccggttca tgccggatgt caggtggaag
     1981 ttgtcatgca gccaccagtt gtcgaggacg gaacatcttt ggacgcaacg gactctggcg
     2041 acgcctcgga ccgcctgtgg tgcatggtgg cgatccattg aatcccgaac ggctccattc
     2101 agcgttcttc cgctcttgtc cgcaccaaga gttgtcacct cccaccaaac ctcggattcg
     2161 tgtgagacga gggccttggc acgcgttgcg tcagggaggg aggggcatgc ctagacagga
     2221 aatgggggag cacttcacgc gctgtgcgat tgacctgccc ttccgctacc gcgaatcgac
     2281 ggaggtcgcc taccgccccg gcgtcggttg gacgcgagac ctgagtggag gcggcgcctg
     2341 ggtcgagttg cccgaacggg tggcgcctcc gaccgaattg gaagttggcc tgcacgcgct
     2401 tggcgaaccg ctgcgcctgg ccgcgcaggt ggtctggggt tgccccgaac cgcacgtgcg
     2461 atcccgactc cacggattgc gcttcattcg catcacgccg gcgcagcgac agcgcttggg
     2521 agtgctcatc acccaccaga aatcgcgtga agttggacgc ctctattgta gcttggccgc
     2581 gacctgccag agcaaggcag ccgctggtgt cccgctaccc tgcgagaccc gggatctctc
     2641 cttcggcggg gtctccctcc gcctgccggc attggcgccg cccggaactc acctgcaggt
     2701 gaagattctc accgcatttg gcgctgtcac ggccgacgcc caagtcgtct gggtcgagcc
     2761 accggacgct cgaccccgga acgccccctg ccgacacggc ctgcggtttc ttcgcctcga
     2821 gccgtcgagc gatctgcccc tgaaagcgct gctggctgga ctgcggtagt cagcggcaat
     2881 catcgcggaa tccggaaacc ccgcgggcct gtgccggcat gtccctgcag tgctttcgct
     2941 acaatcgttg actatcctcc tccctgaaag cgctccaaga ataaccaagc tcatgtgtcc
     3001 ggcaagacaa ctcgcgctga cgcggacgtc ctcgaggcag tccccgccgg ctgcttggat
     3061 ccagacgatc gcttgcagct tgtcgaactc cgcgtagccg cgctggcggg cgcgcacctg
     3121 gagcatgtcg cgcacgagtc cctccccgcc cagcgcgagg agcgtctcgg cgatgagcgt
     3181 cagccccgcg tgcgccgtca gatccatcgg gcgaggcgcc gcttcgaacc ggaagggcag
     3241 gaggccttga cccacgggcg tggagtctct cggcgtcgca tggtcggggg agaatattgc
     3301 gaacgacttg cacgtgtgac cctcttcacc cgatgggtgc agcggatttc acgtccatgg
     3361 cgcgggatcc tcatctgctg ctgagtttca aggtcgaagg tactgtcctc agaataagat
     3421 gttgccgacg atcctggagg gtgatagcct atctgggtaa cggtccgggg caggcggcgc
     3481 gctagtaagc cgaaacctct cgggtgaagg catccgagct ggcaggcctc gacggacaaa
     3541 accgcggaag gatactggca acgtatcagt tgtcttgttc ctttgcgtct tgatcgcgag
     3601 gcactcccag agccaggcag taattatttc acgcttacag tgtatatttt ggatcttcgt
     3661 cttggaacgt cccgtctgga cattccctta actatatgaa gtcattggcg gaaatagact
     3721 ccatactttc cgactggttc ccggcatgat agttgctcac attttagcgg gtttaggctg
     3781 agtaccccgg gagccgcagt cccttgtgtg ttcgaactcc agccagcggg tccgagggcg
     3841 gtccggtcgt cgccaaggtg tagcgtttac taacccggct tgatcccctg gaggctgttc
     3901 aaggaatgag gcgaagtagc cggctctcgc cggcggtctc gcatgacatg ggtggatcgg
     3961 tgctttagag tcctctccgg aatcgtacgg ggtggacggc tccgaacaag taaaggagga
     4021 tcaaggaatg gcttttttta caccgcagcc tcggaaggac gaacctgtgg ccaaacccga
     4081 aataaagcct gagccaaaac ccgagccaaa ggtggagtca ccacgcgagc aggttgcgcc
     4141 acccccaccc acacccatcc gacgcatgga ggagccggta atgtcagcaa ttcagcccaa
     4201 gggagccgaa tcgctcgtgg cgaagggact gacgattgag gggaagattg agggatcggg
     4261 cgacgttcgg attgccggcc gcttcaaggg gaccataaac gtcaagggcg agttcagaat
     4321 cgagtcgggt gccaccatcg agggggaagt gactgcggac accgttctcg tcggcggtga
     4381 ggtcaacgga aagatcgtgg cctcctcacg tgtggagttc aaggaatccg gtagtctcat
     4441 cggagatctg aaggccggta gcttgacggt ggcggcgggc tcgaagatgc gcggcaatgt
     4501 cgaattcggg tggaaagagg gcgaggttga gagtcttccg ctgaaggagc gtggccacgc
     4561 ggccatccgg taggctttga tgggggtgac gggtgagtcc tggaaatgcc ctcactgcgg
     4621 ggagcagatt ctccgaagcg ccgtaatctg cccggcgtgc cagcgtcggc ttcacgtcaa
     4681 tgccctggcg acgcatcaaa ctacccaggt cattgcttcc cccctgaatg tcgagggtgt
     4741 catccgacac cctggcaccg gcgccgcctg ggagtactcg gtgttggttg aagtccagga
     4801 tcaggaaggc gaggtcctgg ccaggcgcgt cgtcggggtg ggagcacttc aacccggcga
     4861 ggctcgggtg gtccggcttc gcgtggacat gcactcacga gaaaaaatca cgcctgcggc
     4921 ggctgccgcg cgacaaccga agagtgagtg agacggacac catccagcga gccaattcgt
     4981 atgcgggaac gtgctgcaaa ttggcgctaa tgtaaacgcc tctgccggac cagcctaacc
     5041 cgtcctctcg tgcagttgac ccaccctcag ggaatctctc tcaccagtag ctccctccat
     5101 gctcctgtcc gggtccagcc gccgtattgg gagctgcctg cgatacgcct tgggggacgg
     5161 ctatcgcccg gtcctcttca accgg
//
LOCUS       DBNGZC010000543         7493 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_437847, whole genome shotgun sequence.
ACCESSION   DBNGZC010000543 DBNGZC010000000
VERSION     DBNGZC010000543.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7493)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7493)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7493
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_437847"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            315..1550
                     /locus_tag="AC16G0_09480"
     CDS             315..1550
                     /locus_tag="AC16G0_09480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5099880.1"
                     /translation="MQPRAEAPVPADATVPADRRYISRLVPLLCAGWAVLYADRTVLY
                     PLLTVIAREFGLSGTQTGLITGTYFTLYVVTLLVSGLLAERFGLKRVLLIFSFVLAVS
                     VAGFGVVALNYLALLLVAGLHGAGGGPYYPMAYSIMIHTVPGRLRGVASGLINGGMSL
                     GLAVGLALAGPVYQATGSWRAPFLILAVPTFLVAVLFQRIVREVPVPLKRSIPLSGLL
                     RDPTLLCMNLAAFCALYGWWVILSWGPVFFQTERGLSLTMSGVYTLVIAITAIPSGLL
                     LGRLSDHIGRKRIILVMLPLMALSLAALPQVRSQAGMFLALMAYGVVGKLAWDPLGIA
                     WLGDYMGQNRPEAVGAAVALFSFISVLSAVVGPPLTGWIKDLTGSLAGGFYLAGGLAL
                     VGFVLSLKPADSRPKRTTL"
     gene            1547..2947
                     /locus_tag="AC16G0_09485"
     CDS             1547..2947
                     /locus_tag="AC16G0_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5099881.1"
                     /translation="MSSPEGHAARPAVMAGPDTGHSSRRVVRLLWALVLVGAVLWTAA
                     GTPAEKPLTPAIRILVLKTEAEARDAVAAFDAGVSFDRLVRERSIGPNAERGGLLGRV
                     DSASLSPEARAALAKTQPGRLSTIFRTDAGFAVIQVVTEQEEQELEARTRREREAREL
                     FTQGTEAARGGDLQGAIPLLQRAAQLDPNLADAHFNLAIAYGRLNRLDAAIAAMREAI
                     RLRPDDVEAHMNLGRWLSSRGLHAEATQAYERAATLRMNSWEAWLRLAQSYDAAGKAR
                     ASVAAYQRVLALLGRDEPAIVRALFRVAMKAGDGPAAVEAAQKLRGFQFGHEGFLSLG
                     EALLLNGQAEDAVREFQKASALVPTSAKAHAGLASAYARLGQREAAAESYRQAIRLDP
                     MNPELYGRLAHVYEEMGRLDLAIVALRDGVSAADLSPAPLQAELAEQLIALYEKAGMT
                     REAGRERLRLQALRAP"
     gene            3148..3852
                     /locus_tag="AC16G0_09490"
     CDS             3148..3852
                     /locus_tag="AC16G0_09490"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR5099882.1"
                     /translation="MADVTPVLLLDGHQSLRERIVRRLRDAIITGDFPATARLREPEL
                     AGRLGVSRTPLREAIRQLEAEGLVNTVPRVGTFVSEVHPQDVENTYAIRAVLEGLAAR
                     QAAENLDPAKAARLREILAELERKQADYRAYHEAAGRFHEVIFALSGNERLQTIYQSL
                     THQVARFRSLSLALPQRPERSLREHKRIATAILRGRGKEAEQLMRVHVEGARAVLRQR
                     MKPVKPSRGPRRGNTR"
     gene            3863..5275
                     /locus_tag="AC16G0_09495"
     CDS             3863..5275
                     /locus_tag="AC16G0_09495"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007253652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR5099883.1"
                     /translation="MSKERVDAGAGSLTAYVADFITSTKYADIPKAVVPLGKKSILDG
                     LGLALAGSVAKSGDLVRRHLQGLGCAGAASVIGSSLRLPARFAAFANGTAVHADDYDD
                     TQLAVAKDRVYGLLTHPTAPVLPAVLAVAECDGRSGRDLLTAYHVGVEVECKIAEAIH
                     PRHYQHGFHTTATCGPLGAAAGLVNLLGLSAQVTQVALGIAGSQSAGLRENFGTMTKP
                     FHAGRASESGVVAAELARLGFTATPHILEAPRGFFHAAGGGYDEGAIRQKLGGPWTFA
                     DPGVSIKPHPSGSLTHPGMTLMLSLIKQNDIRPEQVEHVKVGTNQNMPNALIHHRPTT
                     ELQAKFSMEFCMAILLLERKAGLPEFTDEVVNRPDVQAMIQTVEFGVHPEAEAAGYDK
                     MTSIIEIRLKGGRVISGRADFGKGSPANPMSYEEVADKFRECAAFSRWPSQKAEDVIG
                     MVKSLEALPNVRELTALLSG"
     gene            5387..6376
                     /locus_tag="AC16G0_09500"
     CDS             5387..6376
                     /locus_tag="AC16G0_09500"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR5099884.1"
                     /translation="MKKVFGLAVVTLIASVGIAPSIVGAADFPTKTITAIVTVSPGGS
                     NDIQTRAFASVAEKILKQPVVVINKPAASGMLGLLQGAESAPDGYTLTTSSMSEMCVL
                     EWEKANNRKTEVALEDFVSIGGFTVASLIIGVPVNSPWKTIDDLIKDAKAKPGEYAFA
                     SGGLYRIQHINTELFAQATGVKFRHVPYSGGGQAVTAVVGGHEHFASMTPSSSIPLFK
                     GNKLRALAVQGDRRLKSLPNVPTLKEAIGIDAGVPQIVGLGVPKKTPAPIVERLRAVV
                     KQVSEDPAFINVIESQGDEVRHVSGEEFAKISARESEKVAKLFKQLLLEEKKK"
     gene            6547..6990
                     /locus_tag="AC16G0_09505"
     CDS             6547..6990
                     /locus_tag="AC16G0_09505"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR5099885.1"
                     /translation="MNKTRAGSLVLFVVGAYGFVLSLRLPLGKLNAPGAGAFPLIVSI
                     LLTLSGIFLYVGEGKKVEIKWRELIHEQRTPFQIVVLTAAFILALDRLGYVLSSTAYM
                     FALLYWVSRYRLTVSIALAIALGVGSWYVFGTVFETPLPVGILGR"
     gene            7023..>7493
                     /locus_tag="AC16G0_09510"
     CDS             7023..>7493
                     /locus_tag="AC16G0_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR5099886.1"
                     /translation="MDVVHNLLSGFATAITLKNLYLAAVGCLWGTIVGVLPGIGPVAG
                     ITLLIPATFGIDATGAIIMLAGIYYGAMYGGSTTSILMNIPGESASVITCLDGYQMTL
                     KGRGGAALFIAAFGSWVGGTLSIVGLMLMAPMLAEFATKFGPPEMFAIYMVAFIL"
BASE COUNT         1231 a         2372 c         2550 g         1340 t
ORIGIN      
        1 caaggggcgg tgctgaaggc cgattagcga ggggttcgga actggggctg gggggctcca
       61 atggacgcga gcgtaggggg ctgacccgcg ggacgtcgtg gacagtcaat gcggcggttc
      121 ccttctccga cacgagccgg gcgttctccg ggggacgaat caaccgcagg gggcgggccc
      181 gtaggcgggt tcgccccctg gttttttcgg cagatgatgt gcgcgtgatt tccgggtcgg
      241 ttgcaagcca ggctcccccc ggtcggtggc tgtcgagcca ccgcatcacc gggtgatcct
      301 gcgagggagg cgaggtgcag cccagggcag aagcgccggt gcctgctgac gcgacggtgc
      361 cagccgatcg aaggtatatc tcccgcctcg tgccactcct ctgcgccggc tgggctgtcc
      421 tctacgccga tcgaaccgtt ctctacccac tcctcacggt gatcgcgagg gaattcggcc
      481 tcagcgggac ccaaacgggt ctcatcaccg ggacgtactt cacgttgtac gtggtcacgc
      541 tcctggtgtc cggcctgctg gccgagcgct tcggcctgaa gcgggtcctg ctcatcttct
      601 cctttgtgtt ggccgtgagc gtggcgggat tcggtgttgt tgccctgaac tacctcgccc
      661 tcttgctggt ggccggtctg cacggggcgg ggggcggacc gtactacccc atggcctaca
      721 gcatcatgat ccacacggtc ccgggccgcc tccgcggggt ggcgtcgggt ctgatcaacg
      781 gtgggatgtc cctgggcctg gcggtggggc tggccctggc cggacccgtg taccaggcga
      841 cggggagttg gcgagccccc ttcctgatcc tggcggtccc caccttcctg gtggccgtgc
      901 tcttccagag gatcgtccgg gaggtcccgg tgcccctcaa gcggtcaatt cctctctctg
      961 gattgctccg cgacccgacg ctcctctgca tgaatctggc cgcattctgc gccctgtacg
     1021 gatggtgggt gatcctgtcg tggggtccgg tattctttca gacggagcgg gggctgagcc
     1081 tcacgatgtc gggcgtctac accctggtga tcgccatcac cgccatcccc tccggactgc
     1141 tcttgggccg actgtccgat cacatcggtc gcaaacgaat catcctggtg atgctccccc
     1201 tgatggcctt gagcctggcg gccctgcccc aggtgcgttc ccaggccggg atgttcctgg
     1261 ccctcatggc ctacggcgtg gtggggaagc ttgcctggga ccccctgggc atcgcgtggc
     1321 tgggggacta catggggcag aatcggcccg aggcggtggg ggcggccgtg gcgcttttct
     1381 cgttcatcag cgtgctgtcg gcggtggtcg gcccgccctt gacaggctgg atcaaggacc
     1441 tgacgggctc gctggccggg ggcttctacc tggctggagg tctggctctg gtcggattcg
     1501 tcttgtcgct caaacccgca gacagcaggc cgaagaggac cacgttgtga gctcgccgga
     1561 ggggcatgcg gccagaccgg ccgtcatggc cggccccgac accggccatt cgtcaaggcg
     1621 ggtggtccgg ctgctttggg ctctcgtgct ggtgggggca gtcctctgga cggcagccgg
     1681 aacgccggcc gagaagccgc tcaccccggc gatccggatc ctggtgctca agacggaggc
     1741 ggaggcgcgt gacgcggtgg ctgccttcga tgcgggggtg tcgttcgacc ggctcgtgcg
     1801 cgagcgctcc atcgggccca acgcggaacg gggcggcctc ctcggccggg tggactcggc
     1861 cagcttgtcg cccgaggccc gagccgccct ggccaagacc cagcccggac gattgagcac
     1921 catcttccgg accgatgccg ggttcgccgt catccaggtt gtcacggagc aggaagagca
     1981 ggagttggag gcgcggacta ggcgagagag ggaggccagg gaactattta cccaagggac
     2041 cgaggcggca agaggcgggg acctccaggg ggcgatcccg ctccttcagc gggccgccca
     2101 gcttgacccc aaccttgcgg atgcgcattt caacctggcc atcgcctacg ggagactcaa
     2161 ccgattggat gcagccatcg cggcgatgcg cgaggcgatc cgcctgcgac cggacgatgt
     2221 agaagcgcac atgaaccttg gcaggtggct gtccagccgt ggtctgcatg cggaggcaac
     2281 tcaggcctac gagcgggccg cgacgcttcg catgaattcg tgggaggctt ggctgaggct
     2341 ggcacagagc tatgatgccg caggcaaggc tcgggcttcg gtggctgcgt accagcgggt
     2401 gctggccctg ctcggccgcg acgagccggc catcgtgcgg gcgctcttcc gggttgccat
     2461 gaaggctggg gacggcccgg cggcggtgga ggcggcgcag aaactgcgcg ggtttcagtt
     2521 cggccatgaa gggtttctga gcttgggcga ggcgctcctc ttgaacgggc aggccgagga
     2581 tgccgtccgg gagttccaga aggcttcggc cctggtgccg acctccgcga aagcgcatgc
     2641 cgggttggcc tccgcctacg cgcgactggg ccagcgcgag gcggcggcgg agagttaccg
     2701 gcaggccatc cggctcgatc cgatgaaccc ggagctgtac ggccggctgg cccatgtcta
     2761 cgaggagatg ggtcgcctgg atctggccat cgtggccctc cgggacgggg tgagcgccgc
     2821 cgacctgtct cccgcgccgc tccaggccga actggccgag cagctcatcg ccctgtacga
     2881 gaaggccggc atgacccgtg aggccgggcg ggagcgactt cgcctccagg ccctgcgcgc
     2941 tccctgatcg cggccccctc cggtccgagg attcctcccc tccctcgcct ctggaatcaa
     3001 gcgattcgcg tcgcatcggc catcctgccc gtcggcctct ccaccctgat cccgggccgc
     3061 tccacccggc ggcgacgggg gagattgtgc ttgacacgga tgagcagtat ggagtacaca
     3121 gcatactgta tacaatctgg agatccaatg gccgacgtca ccccggtact ccttctggat
     3181 ggtcaccagt ccctgcggga gcggatcgtc cgccgcctgc gggacgccat catcaccggc
     3241 gacttccccg ccacggcccg cctccgggaa cccgaacttg ccggccggct gggtgtcagc
     3301 cgcacccccc tgcgcgaggc catccgtcaa ttggaggccg aagggctggt gaacaccgtc
     3361 ccgcgggtgg gcaccttcgt cagcgaggtg caccctcaag acgtcgagaa tacctacgcc
     3421 atccgggccg tcttggaggg cctggccgca cggcaggccg cagagaatct ggacccggcc
     3481 aaggccgctc ggctgcgcga gatcctggcg gagctggagc gaaagcaggc cgactaccgg
     3541 gcgtaccacg aggccgcagg gcggttccac gaggtgatct tcgcgctctc ggggaacgag
     3601 cgccttcaga ccatatacca atctctgacg catcaggtgg cgcgcttccg cagcctctcg
     3661 ctggccctcc cccagcgtcc cgagcggtcc ctccgggagc acaagcggat cgccacggcc
     3721 atcctccgcg gtcgggggaa agaggcggag cagttgatgc gggtccacgt cgagggggcg
     3781 cgcgccgtgc tgaggcagcg catgaaaccc gtgaagccaa gccgggggcc gcgtcgaggg
     3841 aacacacgct aaggaggccg ggatgagcaa agagagggtt gacgcaggag ccgggtcact
     3901 caccgcctat gttgcggact tcattacctc cacgaagtac gcggatatcc cgaaggccgt
     3961 ggttcccctc gggaagaagt ccatcctcga cgggttgggc ctggctctcg ccggctccgt
     4021 ggcgaagagc ggcgatcttg tgcgtcgcca cctccagggt cttgggtgcg ctggcgcggc
     4081 cagcgtcatc ggatcctcgc tgcggctgcc tgcccgcttc gcggccttcg ccaacggaac
     4141 ggcggtccac gccgacgact acgatgacac ccagcttgcg gtggccaagg accgggtgta
     4201 cggtctgctg acccatccca cggcgccggt tctgcctgcg gtcctggccg tggcggagtg
     4261 cgacggacgc tctggccgcg acctgctcac ggcctatcac gtgggcgtcg aggtcgagtg
     4321 caagatcgcc gaggcgatcc acccgcggca ctatcagcat gggtttcaca ccacggcgac
     4381 ctgcggtccc ttgggcgcgg ctgcggggtt ggtgaacctg ctggggctct ccgcgcaggt
     4441 gacgcaagtg gccctgggga tcgccgggag tcaatcggcg ggccttcgcg aaaacttcgg
     4501 gaccatgacg aagcccttcc acgcagggcg agcgtcggag agcggggtgg tggcggcgga
     4561 gctggcgcgc ctggggttca cggcgacgcc gcacatcctg gaggcgcccc gggggttctt
     4621 ccacgcggcg gggggcgggt acgatgaggg cgccatccga cagaagctcg gaggcccctg
     4681 gaccttcgca gacccaggcg tctcgatcaa gccgcacccc tccggatcgc tgacccatcc
     4741 cggaatgacc ttgatgctct ccttgatcaa gcagaacgac atccggccgg agcaggtcga
     4801 acatgtgaag gtcggcacga accagaacat gcccaacgcc ctcatccatc accggcccac
     4861 gaccgagctg caggccaagt tcagcatgga attctgcatg gccatcctgc tgctggagcg
     4921 caaggccggc ctgccggagt tcacggacga ggtcgtcaac cgaccggatg tccaggcgat
     4981 gatccagacg gtggaattcg gtgtgcatcc ggaagccgag gcagccggct acgacaagat
     5041 gaccagcatc atcgagatcc ggctgaaggg cgggcgggtc atctccgggc gtgccgactt
     5101 cgggaagggg agcccggcga acccgatgag ctacgaggag gtggcggaca aattccggga
     5161 gtgcgccgcc ttcagccggt ggccgtcgca gaaggccgag gacgtgatcg gcatggtcaa
     5221 gtcactcgag gctcttccca atgtacggga actcaccgcg ctcctttcag gctagccgac
     5281 caccccgatt tatgacgacc ctggctcctg ccatgctcgg cccggatctg gatcagatgg
     5341 caagagggtg ttggatggcc acgacatcat tcatggaggt attgcgatga aaaaggtctt
     5401 cggtctggcg gtcgtgactc tcatcgcatc tgtgggcatc gccccttcca tagtgggcgc
     5461 ggccgatttc ccgacaaaga ccatcacggc catcgtcacg gtatcgccgg ggggaagcaa
     5521 tgacattcaa acccgggcct tcgcgtctgt agcggagaag attttgaagc agcccgtcgt
     5581 ggtcatcaac aagccggccg catccgggat gctcggcctg ctccaggggg cagagtccgc
     5641 tccggacggc tacaccctga ccaccagctc catgagcgag atgtgcgttc tggagtggga
     5701 gaaagcgaac aaccggaaga ccgaagtcgc cctcgaggac tttgtctcca tcggaggctt
     5761 taccgttgcg tcgctgatca tcggagtccc cgtgaacagc ccgtggaaga ccatcgacga
     5821 cttgatcaag gacgccaaag ccaagccggg agagtacgcc tttgcctcgg gcggcctgta
     5881 ccgcatccag cacataaaca ccgagctctt cgcgcaggcc acgggcgtga agtttcggca
     5941 cgtcccgtac agcgggggag ggcaggcggt cacggcagtc gtgggcggcc acgagcattt
     6001 cgcctcgatg acgccgtcct cttcgattcc gctcttcaag ggcaataagc ttcgggcctt
     6061 ggccgttcag ggtgaccgga ggctgaagtc tctgccgaat gtcccaacct tgaaggaagc
     6121 gatcggcatc gatgcgggtg tgccgcaaat cgtgggtctg ggcgtcccca agaagacacc
     6181 cgcgcccatt gtggaacggc ttcgcgcggt cgtgaaacag gtgtccgagg acccggcctt
     6241 catcaatgtc atcgagagcc agggcgacga agtgcgccac gtcagcggag aggaattcgc
     6301 caagatctct gcccgggaat cagagaaggt ggcgaagctc ttcaagcagt tgctgctcga
     6361 ggaaaaaaag aagtaattgt tgcagagatc tgccggttgc ccgtgtcaac cgagggcctc
     6421 gtcggaccga gagggggtat tcggcaggct gccgctccgg ggtcaatcac gacgaccgaa
     6481 cgcgggattg cccctcttgc ctttgctgag tgggaagaac ctgacttcat tcgaagggcc
     6541 acgccgatga acaagacgcg cgctggcagc ctggttctct tcgtggtggg agcatacgga
     6601 tttgtgctct ccctccggtt gcccctgggg aaattgaacg cgccaggcgc cggagcattc
     6661 ccgctcatcg tttcgatcct gctgacgcta tcaggtatct tcttgtatgt cggcgaaggg
     6721 aaaaaggtcg agatcaagtg gcgagagctc atccatgagc agcggacgcc gttccagatc
     6781 gttgtcctga cggcggcatt tatcctggcg ctggacaggc tcgggtatgt gctctcgtcc
     6841 acggcctaca tgttcgccct cctgtactgg gtgagtcgct accggctcac ggtctccatt
     6901 gccctggcca tcgctctcgg cgtggggagc tggtacgtgt tcggcacggt gttcgagacc
     6961 cctctgccgg tgggaatcct ggggcgctaa ggatcggaac ggccagaccg ggagtcgcct
     7021 gaatggacgt tgtgcataac ctgctgagtg ggttcgcgac agccatcacg ctcaagaatc
     7081 tttacctggc tgccgtcggg tgcctgtggg ggacaatagt cggcgtcctg cccggcattg
     7141 ggcccgtcgc aggtatcacg ttgctcatcc cggccacttt cgggatcgat gccaccgggg
     7201 cgatcatcat gctggccggg atctactacg gggccatgta cgggggttcc accacctcga
     7261 tcttgatgaa cattccgggg gagtccgcct cggtcatcac ctgcctcgac ggctatcaga
     7321 tgaccttgaa agggcgaggc ggggccgccc tgttcatcgc ggcattcggc tcgtgggttg
     7381 gcggcacctt gagcatcgtc ggcctgatgc tcatggcgcc gatgttggct gaatttgcca
     7441 cgaagttcgg tccgccggag atgttcgcta tctacatggt cgccttcatt ctg
//
LOCUS       DBNGZC010000544         5473 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2001875, whole genome shotgun sequence.
ACCESSION   DBNGZC010000544 DBNGZC010000000
VERSION     DBNGZC010000544.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2001875"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="AC16G0_09515"
     CDS             complement(<1..1172)
                     /locus_tag="AC16G0_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099887.1"
                     /translation="MKRALVLLLALSPVLLLFGSSPAFAQSRADVLVIGMSTSDITSL
                     DPAKAFEFAGTGVVSQVYDRLLDFPAGRYDKPEPWLAESWKVSADGLVWTFSLRKDAK
                     FHSGNQVTADDVVYSFQRVILLKDQPAFILTQFGMTPESIRALDKSTVQITVPKKFAG
                     GLFLACMTAAVASIVDSQVVKQHITKTDKFPNGDFGLTWLSQNSAGSGPFILRKWEKG
                     DSVVMDANPSHYLHPPKVKRVVIKEIPEATSRRLQVEKGDLDVAWQMLPDQVKEMAKN
                     KDLHIDKFPATQIVYMGLNVTHGPLADNRVRQAIKYAVDYKGIIDDVLGGAAKPINTF
                     IPEGFAGYESRLIYKTDLTKARQLMKEAGQANGFETTLDHAEFTPIPEVAQVIQNSL"
     gene            complement(1566..2879)
                     /gene="gabT"
                     /locus_tag="AC16G0_09520"
     CDS             complement(1566..2879)
                     /gene="gabT"
                     /locus_tag="AC16G0_09520"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - GABA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HIR5099888.1"
                     /translation="MSTHTEKLAERRNRQVPRGVGNLNPVFIAKADGALLTDVDGREY
                     IDFAGGIGVNNVGHRHPKVLAAIQEQLQAYLHPCFHVMMYEPYVELAERLNQIAPCRG
                     EKKTMFVNSGAEAVENAVKIARYHTRRSGVVCFDNAFHGRTLLTMTLTSKVRPYKLHL
                     GAQAPGIFRAHFPYCYRCPWGLTYPKCNVPCGEAYFEEDFFKHHVDPEEVAAIILEPV
                     QGEGGFITAPPEYLGQIRRVCDRHGILLILDEVQTGFGRTGKMFAAEHFGVQADIVTS
                     AKSLAGGLPLGAVTGTAAIMDSVHSGGLGGTYSGNPLACRAALAVLDVMEEEHIVARA
                     AELGQRVRKALTGLYDTYAIIGEVRGLGAMLAIELVKDRASKKPAAEDAKKLTAFCRE
                     HGLIILDCGTLGNNIRTLMPLTITSDQLAKGLGILEEGLKQLSVR"
     gene            complement(2895..3605)
                     /locus_tag="AC16G0_09525"
     CDS             complement(2895..3605)
                     /locus_tag="AC16G0_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099889.1"
                     /translation="MSLLSIESIDVFYGPAQALWGVSFAVEKGQVASLIGTNGAGKTT
                     TLKALCGLITLAAGRVTYDGEVISGLPVHQIMDRGVSLVPEGRQLFPKMTVEENLAIG
                     SYLSRTKPQRKVNLERVYHVFPRLAERRLQIAETLSGGEQQMLAIGRALMQEPKLLIL
                     DEPSLGLAPVLVREIFGIVKDLHGRGLTILLVEQNVHQALQVADIAFVLENGRVVMSG
                     TGLELISDPKVKEAYLGF"
     gene            complement(3602..4348)
                     /locus_tag="AC16G0_09530"
     CDS             complement(3602..4348)
                     /locus_tag="AC16G0_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099890.1"
                     /translation="MAEPVLRVDGVTVAFGGLVAVNHVSFHVAAGEIVGLIGPNGAGK
                     TTLFNAVSGYYKPTRGRITFLEHDITGRQPYALAARGIGRTFQIVKPFAGLSVLQNIL
                     VASYLRYPKQVDAREKAWEVLKFIGLGDRADLPAAGLTLAGRKRLEIGKALALEPKLV
                     LLDEVVAGLNPTEADQTVQLILKIRDAGMTILIVEHIMRVIMKISTRIIVLNYGKMIA
                     QGTPEEVAKSREVIEAYLGEGAGTANGSAA"
     gene            complement(4358..5329)
                     /locus_tag="AC16G0_09535"
     CDS             complement(4358..5329)
                     /locus_tag="AC16G0_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5099891.1"
                     /translation="MRGRPAWLWILLVIAGVGLVPVVMRDAFFLRLFTEALMWIGLAI
                     TWDIMAGYIGYLNFGHGVFFGVGAYTTALLIIKTGLPFGPAAALGGVAAGVVALVTGW
                     PTLRLRGSYFAIGTWALNRAVMQLALVLDITGGADGMRLPPFLRPQFFSWVMTGVVGA
                     TFLVLWLWLEQAPFGLKLKAIREDESGSLAIGLNPAKLKLQAFVLSAVPAGIIGGVYA
                     YWITFIDPSSTLGDLVTDQAVVMAIFGGLGTLIGPVIGAILFFAFKTYFWAYLSDYQV
                     LYLIILGLAIAATVFFLPDGFWGTVARRQEGRRALQKGLALRDTKAT"
BASE COUNT         1038 a         1713 c         1751 g          971 t
ORIGIN      
        1 agggagttct gaatgacttg ggccacctcc ggtattggcg tgaattccgc gtggtccagc
       61 gtggtttcga aaccgttggc ctggccggct tccttcatca gttggcgggc tttggtcaga
      121 tcggtcttgt agatgaggcg cgattcataa ccggcgaatc cctccgggat aaacgtgttg
      181 atgggtttgg ccgcaccccc caggacatcg tcaatgattc ccttgtaatc aacggcgtac
      241 ttgatggcct ggcggacccg attgtcggcc agaggaccgt gagtgacgtt gagccccatg
      301 tacacgatct gggtcgccgg gaacttatcg atgtgcaggt ccttgttctt ggccatctcc
      361 ttgacctggt ccggcagcat ctgccaggcc acatccagat cccccttctc cacctgcagg
      421 cgtcggctag tggcctcggg aatctccttg atcaccaccc gcttgacctt aggcggatgg
      481 aggtagtgac tcgggttggc gtccatgacc accgagtcgc ccttctccca cttacgcaag
      541 ataaatggcc cggacccggc cgaattctgg gagagccacg tgaggccgaa gtcgccattg
      601 gggaacttgt ccgtcttggt gatatgctgc ttcaccacct gggaatccac gatgctggcg
      661 acggccgctg tcatgcaggc gagaaagaga ccgccggcga acttcttcgg caccgtgatc
      721 tgaaccgtag acttgtccag ggcgcggatc gattccgggg tcatgccgaa ctgagtgagg
      781 atgaacgccg gctggtcctt gagcaaaatc actcgctgaa aggagtagac gacgtcatcg
      841 gcggtcacct ggttgccaga gtggaatttg gcgtccttcc gcaggctgaa cgtccagacc
      901 aggccatcag cggatacctt ccaggactcg gcgagccacg gctcaggctt atcgtagcgc
      961 cccgcgggaa aatccagcag ccgatcgtac acctgggaca ccacacccgt gccagcgaac
     1021 tcaaaggcct tggccgggtc cagggaggtg atgtcgctgg ttgacatgcc gatcaccagg
     1081 acatcggccc ggctctgggc aaacgcagga gaggaaccga aaagcaggag gactggggag
     1141 agtgcgagaa ggagaaccag tgcccgtttc attaccaccc tccttgcctg tggtcgcgga
     1201 ccggccacac agaccagccc gtgaccgttt ctgggttccc aggcgccatc cgagccggac
     1261 atggccggcc caagatcgtc gcctgggtcg cggagtgagt cgtcttcggc attcaggttc
     1321 ccgatggcgg atcgggatgc gtttcgtgcg tccagctggc aaccggagcc tatcgcgcca
     1381 cagcggattg tcccagccag ggaagcatcg cttgtgccga acacgattgt caatcagaaa
     1441 cttcgcgata tgggaaggca gagcaggtcg gcatcctggt actgccgagg cgagcggaca
     1501 gggacggcga ccacacggcg actgatgcca agccgcagag atgccctgcc gaccacggga
     1561 tggtcttatc ggacggagag ttgcttcagg ccttcctcca ggatgcccag acccttcgcc
     1621 aactgatccg aggtgatggt cagcggcatg agggtacgga tgttgttccc cagggtcccg
     1681 cagtccagga tgatcaggcc gtgctcccgg cagaaggcgg tgagtttttt cgcgtcctcg
     1741 gcagccggtt tcttgctggc gcgatccttg acaagctcga tggcaagcat ggcgccgaga
     1801 ccccggacct cgccgatgat cgcgtaggtg tcgtagagtc ccgtcaacgc cttccgcact
     1861 cgctggccca gctccgccgc acgggccacg atgtgctcct cttccatgac atccaggacg
     1921 gccaaagccg ctcggcacgc caggggattg ccgctgtagg ttccacctaa gccgccggag
     1981 tggaccgagt ccatgatggc ggccgtgccg gtgaccgccc ccagcggcag cccgccggcc
     2041 agagacttgg ccgaggtgac gatgtcggcc tgcacgccga agtgctccgc ggcgaacatc
     2101 ttgcccgtcc ggccgaaccc ggtctgcacc tcgtcgagga tgagcaggat gccatgacgg
     2161 tcgcagaccc gccggatctg gccgagatac tcgggcggcg cggtgatgaa tcccccttca
     2221 ccctggaccg gctccaggat gatggccgcc acttcttccg ggtcgacgtg gtgcttgaag
     2281 aagtcctcct cgaagtacgc ctcgccgcag ggcacattgc acttgggata cgtgagcccc
     2341 caggggcagc gatagcagta ggggaagtgc gcccggaaga tgccgggcgc ctgcgccccc
     2401 aggtgcagct tgtagggccg caccttgctg gtcagggtca tggtcaggag cgtccggccg
     2461 tgaaacgcgt tgtcgaagca gaccacgccg gagcggcggg tgtgataccg ggcgatcttg
     2521 acggcgttct caaccgcctc ggcgcccgag ttcacgaaca tcgtcttctt ctcgccgcgg
     2581 caaggcgcga tctggttcag ccgctcggcc agctcgacat aaggctcgta catcatgacg
     2641 tggaagcagg gatggaggta agcctggagc tgctcttgga tggccgccag gaccttggga
     2701 tggcggtggc cgacgttgtt gacgccgatg ccgccggcaa agtcgatata ctcccgccca
     2761 tccacgtcgg tgagcagggc gccgtcggcc ttggcgatga acacggggtt caggttgccc
     2821 actccgcgcg gcacctgccg gttgcgccgc tcggccagtt tctccgtgtg tgtcgacatg
     2881 ctggctcctt tccctcagaa ccccaggtag gcctccttca ccttggggtc actgatcagc
     2941 tcgagacccg tgcctgacat gacgacccgg ccgttctcga ggacgaaggc gatgtcggcc
     3001 acctgcagcg cctggtggac gttctgctcc accaagagga tggtcagccc gcgtccatgc
     3061 agatccttca cgatcccgaa gatctcgcgg accaacacgg gggctaggcc cagactcggc
     3121 tcgtcgagga tcaggagctt gggctcctgc atcaaggccc ggccgatggc cagcatctgc
     3181 tgctcgccgc cgctcagggt ctccgcgatt tgcaggcggc gttccgccaa gcggggaaag
     3241 acgtggtaga cccgctccag gttgacctta cgctgcggct tggttcggga tagataggat
     3301 ccgatcgcca ggttctcctc cacggtcatc ttggggaaga gctgccggcc ctccgggaca
     3361 agggagacac cccgatccat gatttgatgg accggcaggc cactgatgac ctctccgtcg
     3421 taggtcacac ggccagccgc gagggtgatc aggccgcaga gtgctttgag ggtagtggtc
     3481 ttcccggcgc cgttggttcc gatcagggag gccacctgcc ctttttcgac ggcgaacgac
     3541 acaccccaga gggcctgggc cgggccgtag aagacgtcga tggattcgat gctgagcaga
     3601 ctcatgctgc ggacccgttg gccgtcccgg cgccttcgcc caggtaggct tcgatgacct
     3661 cgcggctctt ggcgacctcc tccggcgtgc cctgggcgat catcttgccg tagttgagga
     3721 cgatgatccg ggtgctgatc ttcatgatga cccgcatgat gtgctcgacg atgaggatgg
     3781 tcatgccggc atcgcggatc ttcaggatga gctggacggt ctggtcggcc tcggtggggt
     3841 tgaggccggc cacgacctcg tccaggagga cgagcttcgg ctccagcgcc agcgcctttc
     3901 cgatctccag acgcttgcgg ccggccaggg tcaggccggc ggccggaagg tcggcccggt
     3961 cacccagacc gatgaacttg agcacctccc aggccttctc cctggcatca acctgcttcg
     4021 ggtagcgcag gtacgaggcg accaggatat tctgcaggac gctgagcccg gcgaagggtt
     4081 tgacgatctg aaaggtccgg ccgatgcccc gggcagccaa cgcatacggc tgacggccgg
     4141 tgatgtcatg ttccaggaag gtgatgcggc cgcgagtggg cttgtaatac ccgctgacag
     4201 cattgaacag ggtggtcttg ccggcgccgt tggggccgat gaggccgacg atctctccgg
     4261 cggcaacatg gaaggacaca tggttcactg ccaccaagcc gccgaaggcc acggtcacgc
     4321 catctactct cagcaccggc tctgccacgt aagctcctta cgtcgccttg gtatccctga
     4381 gcgccagccc cttctgcaag gcccgccggc cttcctgacg cctggccacc gtgccccaga
     4441 agccgtcggg gaggaagaat actgtcgccg cgatggccaa accgaggatg atgaggtaca
     4501 gcacctgata atcggagagg taggcccaga agtaggtctt gaaggcgaag aacaagatcg
     4561 cgccgatgac cgggccgatc agggtgccca gtcccccgaa gatcgccatc accacagcct
     4621 ggtcggtgac gaggtctccc agcgtggacg aagggtcaat gaaggtgatc cagtaggcgt
     4681 aaacgccgcc gataatccca gccggtacgg ctgacaggac gaacgcctgg agcttgagct
     4741 tggcgggatt gaggccgatg gccaggctgc ccgactcgtc ctcgcggata gccttgagct
     4801 tcagcccgaa gggggcctgc tccaaccaca gccacagaac caggaaggtt gcgccgacca
     4861 ccccggtcat gacccaggag aagaactgtg gacgcaagaa agggggcagc cgcatgccgt
     4921 ccgctccgcc ggtgatatcc aacaccagcg ccaattgcat caccgcccgg ttcaatgccc
     4981 aggtgccgat ggcgaagtag gagccccgca gacgcagggt gggccacccg gtgacgaggg
     5041 ccaccacccc ggctgccacg cctccgagcg ccgccgccgg cccgaagggt aagccggtct
     5101 ttatgatgag caaggccgtg gtgtacgcgc ccacgccgaa gaagaccccg tggccaaagt
     5161 tgaggtatcc aatgtacccg gccatgatgt cccaggtgat ggccaggccg atccacatca
     5221 gcgcctcggt gaacagccgc aagaaaaagg cgtcccgcat caccacgggc accaggccga
     5281 cgcctgcgat gaccagaaga atccacagcc atgccggacg tcctcgcatc ctagactccc
     5341 ttgcggaaca tcccggtcgg cgaaatcaac aggacggcat acaagatggt gaatagggcc
     5401 aagagtacgt agctggggtt gaagtagatc aagaaaaagg actggatcag gcccagtagg
     5461 aacgccgccc agg
//
LOCUS       DBNGZC010000545         2654 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2297801, whole genome shotgun sequence.
ACCESSION   DBNGZC010000545 DBNGZC010000000
VERSION     DBNGZC010000545.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2297801"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1198
                     /locus_tag="AC16G0_09540"
     CDS             128..1198
                     /locus_tag="AC16G0_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR5099892.1"
                     /translation="MVTIKDVAREAGVSVGTASQALRGSAVVREATRGRVLAVATRLR
                     YQPSALARGLVTRRTHTVGLLISDIANPFFIRAVRAVEDVAQENGYNVILCNTDEDPA
                     KETRYLRVLMEKRVDGIILATTAASLRMVRDIRWRRIPLVLFDRELPGVTADIVKVDG
                     VLGGRLATEHLLDFGHRRIAIIHGPLVRSTGAERLEGYLTALRAAAVRPDPALIQEGN
                     FKQDSGRELTRRLLELSPRPTALFCTNNLMTVGALQALREGRVRIPENLSLVGYDDME
                     WWTLTQPPLTTVGQPVYDLGREAMRLLLAQIARKTVRRPQRVVLKPELVLRDSCGAPP
                     RLVRTTGSRRRTPRPQSREGHP"
     gene            1195..>2654
                     /locus_tag="AC16G0_09545"
     CDS             1195..>2654
                     /locus_tag="AC16G0_09545"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HIR5099893.1"
                     /translation="MTYLIGLDVGTQSVRSCLFDLDGAVVASASRPLRTIFPKPAWAE
                     QDPEEWWRGAVETLKEILQVSKVNPREIAALSYDCTACTVVALTGEGKALRPALLWMD
                     ERAHVEAEEVSATAHDILKYCGGKVSPQWMISKGRWLERHDPAAFERARWIVEEADFF
                     TYRLTGRMTASLDNATAKWNYVRPLGGWPVDFLRAAKAERLATKWPTQVIPVGRSLGR
                     IHEAVAREVGLSPETVVAQGGIDAHAGEIALGAVGAGDLALIMGSSTCHMAQSKNPVF
                     ASIWGPYPDALVEGMFTLEGGQTASGSIIQWLVEHFGAEATRQAKSRSLDVYAYLDGL
                     AESVPPGSEGLLVLDYFQGNRTPYKDPLARGTVIGLTLKHTLPHLYRAVYEGVCFGTR
                     QILEDMATHGFRLERIYAGGGGAKSRLWTQIQADVLGCPIHLPRDTETMALGAAIWAG
                     LGAGLFQDYRDAIGRMVHIERALSPDPKRHAAYEGL"
BASE COUNT          433 a          866 c          920 g          435 t
ORIGIN      
        1 tttccgccgc atcgccatgt ccgcaccgcg atccccccaa ggtctctgat tgctcttcgc
       61 cttgcacccc gggtgggcga gtgctggccc gtggattgaa acgtttcaac gtgtcgaggt
      121 cggggcgatg gtgacgatca aagacgtggc gcgagaggcc ggggtctccg tgggcacggc
      181 ctcgcaggcc ctgcggggga gcgccgtcgt tcgtgaggcc acccgggggc gggtcctcgc
      241 ggtggccacc cgcctccgct accagcccag cgccctggcc cgcgggctcg tcacccggcg
      301 cacccacacc gtgggactcc tcatctcgga catcgccaat cccttcttca tccgcgcggt
      361 ccgtgccgtc gaggacgtgg cgcaggagaa cggttataac gtgatcctgt gcaacacgga
      421 cgaggacccg gccaaggaaa cacgatacct ccgcgtcctg atggaaaaac gggtggatgg
      481 gatcatcctg gccaccacgg ccgccagcct tcggatggtc cgggatatcc gctggcggcg
      541 catcccgctg gtcctcttcg atcgcgagtt gccgggggtg accgccgata tcgtcaaggt
      601 ggacggcgtc ctcgggggcc ggctggccac ggagcacctc ctggatttcg gtcaccggcg
      661 gatcgccatc atccacggac ccctggtgcg atcgaccggg gccgagcggc tggaagggta
      721 cctgaccgcc ctgcgcgcgg ctgccgtccg acctgacccc gccttgatcc aagaggggaa
      781 cttcaagcag gatagcgggc gcgagctgac ccggcggctc ctcgaactct cgccgcggcc
      841 gacggcgctc ttctgcacga ataacctcat gaccgtgggc gccctccagg ccttgcggga
      901 gggcagggtt cggatccccg agaacctctc cctggtgggc tacgacgaca tggagtggtg
      961 gacgctcacc cagcccccgc tgacgacggt gggtcagccg gtctatgacc tcggccggga
     1021 agccatgcga ttgctccttg cgcagatcgc caggaagacc gtgcggcgcc cccagcgggt
     1081 ggtgctgaag ccggagctgg tcctccggga ctcgtgtggg gctcctcctc ggctcgtccg
     1141 cacaaccggg tcgcgtcgga gaacccccag gcctcagtca agggagggtc atccatgacc
     1201 tacctcatcg gtctcgacgt ggggacgcag agcgtccgga gctgtctctt cgatcttgac
     1261 ggcgcggtgg tggcctcggc cagtcggccg ctccgcacaa tatttcccaa gcccgcctgg
     1321 gccgagcaag atcccgagga gtggtggcgg ggcgcggtcg agaccctcaa agagatcctc
     1381 caggtatcca aggtgaaccc gcgggaaata gccgcactgt cttacgactg caccgcctgc
     1441 acggtggtgg ccctgacggg ggaggggaaa gcacttcgcc cggccctcct gtggatggac
     1501 gagcgggccc acgtggaagc ggaagaagtc tcagccacgg cacacgacat cctcaagtac
     1561 tgcgggggaa aggtgtcccc gcaatggatg atctcgaagg ggcgctggct ggagcggcac
     1621 gatccggcgg ccttcgagcg cgcccgctgg atcgtggagg aggcggactt ctttacctat
     1681 cgcctcacag ggcggatgac cgccagtctg gacaatgcga ccgccaagtg gaactacgtc
     1741 cgtcctctgg gcggatggcc ggtcgacttc ttgcgggcag cgaaggcgga gaggctggcg
     1801 acaaagtggc cgacccaggt gattccggtt ggcaggtccc tggggcggat ccacgaggcg
     1861 gtggcgcgtg aggtgggcct gtctcccgag accgttgtgg cccaaggggg gatcgacgcc
     1921 cacgccggcg agatcgccct gggggccgtg ggggccggcg acctggcact catcatgggg
     1981 agttccacct gtcacatggc tcaatcgaag aatccggtct tcgccagtat ctggggtcca
     2041 tacccggacg ccctggtcga aggcatgttc accctggagg gcgggcagac cgcctccggc
     2101 agcattatcc agtggctggt ggagcatttc ggggccgagg cgacacgtca ggccaagagt
     2161 cgcagcctgg atgtgtatgc gtatctggat ggcctggccg agtccgtccc gcctggcagc
     2221 gaggggctgc tcgtcctgga ttatttccag gggaatcgca ccccctacaa ggatccgctg
     2281 gcacgcggga ccgtgatcgg gctgacgctc aagcacacgc tcccccacct gtatcgtgcc
     2341 gtgtacgagg gggtgtgctt cggaacgcgg cagatcctgg aggacatggc gacccacggc
     2401 ttcaggctgg aacggatcta cgccggcggc ggcggcgcca agagccgact ctggacccag
     2461 atccaggctg atgtgctcgg atgtccgatt cacctccccc gcgacacaga gaccatggcg
     2521 ctgggcgccg ccatctgggc ggggctgggc gcggggttgt tccaggacta ccgggatgcc
     2581 atcggccgca tggtccacat cgagcgggcg ctgtcgccgg acccgaaacg tcacgcggcc
     2641 tacgaggggc tgta
//
LOCUS       DBNGZC010000546         1446 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2198619, whole genome shotgun sequence.
ACCESSION   DBNGZC010000546 DBNGZC010000000
VERSION     DBNGZC010000546.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2198619"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="AC16G0_09550"
     CDS             complement(<1..691)
                     /locus_tag="AC16G0_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099894.1"
                     /translation="MPTDLFLDRLVTRREVERRILARLLSAALVCLLVAWLLLFIAYP
                     VVVMLARSLAAKDGGLTLVHFQTFFGKGYFLRSLGNTLILGITVTVLVLGLGFALAYL
                     VTRGPRLLRWPLRAVALAPLIAPPYIFGLALIIVAGRRGFLAQLLGTQIPIYGWLGVI
                     LAQVLSFLPVAFLMIENVLQSLDPNLEDSACDLGAGELTLLKTILLPLASPGLLKAAL
                     LTFGLAIADFGN"
     gene            complement(725..>1446)
                     /locus_tag="AC16G0_09555"
     CDS             complement(725..>1446)
                     /locus_tag="AC16G0_09555"
                     /inference="COORDINATES: protein motif:HMM:NF024739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5099895.1"
                     /translation="GKREGLWAPYSDAPAWRDIHPRYKDKDGMWFDLGTSSFVLVGNK
                     DLLAKKGYKLPESWFDHLDPKWKGEIVMPSPVTSGTAFMINASFLQLLGWDKGWEFLE
                     KLDKNVAQYTKSGNAPLEHVGRGEFILGITSDEKVPQRLTQGFPIYWSAPKEGIGYEG
                     NIITIVKGTKHLDAAKRIVNFAGTEKFQRWVAQFGYLSARDAPNPMYPTKPKFIDYSY
                     EWAADNHARVIETWKTKFLRK"
BASE COUNT          284 a          436 c          479 g          247 t
ORIGIN      
        1 ggttgccgaa gtcggcgatg gccagaccga aggtgagaag ggcggccttg agaagacccg
       61 ggctggccag ggggaggagg atggtcttga gcagcgtcag ctcccccgcc cccaggtcgc
      121 aagcggaatc ctccagattc gggtcgagcg actgcaggac gttctcgatc atcaggaatg
      181 ccaccgggag gaaggagagg acctgagcca ggatgacgcc gagccaccca taaatcggga
      241 tctgtgtccc gaggagctgg gccaggaatc ctcggcgccc ggcgacgatg attagggcca
      301 ggccgaagat gtacggaggc gcgatgagcg gcgccaatgc gactgcccgc aggggccaac
      361 ggagcaggcg aggcccccgc gtgaccagat aggcgagggc gaagcccagc cccaagacca
      421 gcaccgtgac cgtgatcccc aggatcaaag tgttgcccag ggaccgcagg aaatagccct
      481 tcccgaagaa ggtctggaag tggaccaggg tgaggccccc gtctttggct gcgaggctgc
      541 gagcaagcat caccaccacg gggtaggcga tgaagagcaa gagccacgcc accaggaggc
      601 atacgagggc cgcagacagg aggcgcgcca ggatgcgtct ttccacttcc cgtcgggtaa
      661 ccaggcggtc gaggaaaagg tccgtgggca tgcggctggg ctcgtccggc gcggcccctc
      721 ggggctattt tctcaggaac ttggtcttcc aggtctcgat cacgcgggcg tggttgtcgg
      781 cagcccactc ataggaatag tcgatgaact tgggcttcgt cggatacatg ggattggggg
      841 cgtcacgggc cgagaggtaa ccgaactggg ctacccagcg ctgaaatttc tcggtgccgg
      901 cgaagttcac gatccgcttg gccgcatcga gatgcttcgt acccttgacg atcgtgatga
      961 tattcccctc gtagccgatt ccctccttgg gggcggacca gtagatgggg aagccctgcg
     1021 tgagccgctg cgggaccttt tcgtccgagg tgatccccag gatgaactcg ccccgcccga
     1081 catgctccag gggcgcgttc ccgctcttgg tgtactgggc cacgttctta tccagctttt
     1141 ccagaaactc ccatcccttg tcccagccca agagctgtag gaagctggcg ttgatcatga
     1201 acgccgtgcc ggaagtcacc ggagagggca tcacgatctc gcccttccac ttgggatcga
     1261 ggtggtcgaa ccacgactcg ggcagcttgt agcccttctt ggccaggagg tctttgttac
     1321 cgaccaggac aaacgacgag gtgccgaggt cgaaccacat cccgtccttg tccttgtacc
     1381 gcgggtgaat gtcacgccag gccggggcgt ccgagtatgg cgcccacagg ccttcccgct
     1441 tcccca
//
LOCUS       DBNGZC010000547         1535 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1907681, whole genome shotgun sequence.
ACCESSION   DBNGZC010000547 DBNGZC010000000
VERSION     DBNGZC010000547.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1907681"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1535)
                     /locus_tag="AC16G0_09560"
     CDS             complement(<1..>1535)
                     /locus_tag="AC16G0_09560"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HIR5099896.1"
                     /translation="QQLSKSLFLSPERTLSRKLKEIQIAREIEQRYSKDKILEMYLNT
                     IYFGGGAYGVEAAARTYFNKSVGQLSLAEAAMLAGLPKAPSLYSPFNDLKRAKERRDY
                     VLSRMEKEGDITAAQAKATKRLPVSLSSFFKVRGLGPYFVDYVRKELEPKYGRVLLAR
                     GGLRIYTTLDLKMQQEAAEVLWTGIKGIEKAKAGKKKAPSSDLSGLEGALVALDPTTG
                     DIRAMVGGLDYGRSQFNRAVQARRQSGSAFKPFVYAAAFDRGFTPATIMDDYPISYSI
                     PQNGHMVEWRPENYDRQYRGPVTLRRALEESINVPTVRLMEAVGVDPVVRLAHRMGIT
                     SELRREYALALGVSEVSLLELVSAYGVFADMGLHVPPRAVRRVVAPNGEVLETGGGSP
                     ERVLREEVAFQVTSILEGAVERGTAKLAKIRGREVAAKTGTSQDASDLWLMGYTPGLV
                     VGLWAGFDQPHSLGSHETAGRLIAPIWADLMRRALAEVPPETMPIPEGILPVRVDWRT
                     GLP"
BASE COUNT          267 a          532 c          456 g          280 t
ORIGIN      
        1 gggcaggccg gtccgccaat ccacgcgcac cgggaggatg ccttcgggaa tcggcatcgt
       61 ctcgggcgga acctccgcga gtgcacggcg catgaggtcg gcccagatcg gggcgattaa
      121 tcggcccgcc gtctcgtggg aaccgaggga gtgtggctgg tcaaagcctg cccacaatcc
      181 caccaccagg cccggggtat agcccatgag ccagaggtcg ctggcgtcct gactcgtccc
      241 cgtcttggcg gcgacctcgc ggccacggat cttcgcgagc ttggcggtcc cccgctccac
      301 cgcaccttcc aggatgctgg tgacttggaa cgctacctcc tcgcgcagga cgcgctccgg
      361 actgccgccg cccgtctcca gaacctcccc attcggggcg accacccggc ggacggcccg
      421 cggcggaaca tgtagaccca tgtcggcaaa caccccgtag gccgagacca gctccaacag
      481 acttacctct gacaccccca gggccagcgc atactctcgc cgcaactccg aagtgatccc
      541 catccgatgg gccagtctga ccaccggatc gactcccacc gcctccatca aacggaccgt
      601 cggaacattg atggattcct ccaaagcccg ccggagggtt accggtcccc ggtattggcg
      661 gtcataattt tccggcctcc actccaccat gtgcccgttc tgcggaatcg aataactgat
      721 cgggtagtca tccatgatcg tggccggcgt gaagccccgg tcgaaagccg ccgcgtacac
      781 gaatggcttg aaggccgagc cggactgacg ccgcgcctgc accgcacggt tgaactggct
      841 acgcccgtaa tccaggccgc ccaccatggc ccggatgtca cccgtcgtag gatccagagc
      901 caccaaggcc ccctccagtc cggacaggtc ggacgagggg gccttcttct tcccggcctt
      961 ggccttctcg atgcccttga tgccggtcca caagacctcg gcggcctctt gctgcatctt
     1021 caggtcgagc gtcgtgtaga tccggaggcc gcccctggcc aagaggacgc gcccgtactt
     1081 tggctccaac tccttgcgca cgtagtccac gaaatacggt cccaggcccc gcaccttgaa
     1141 gaatgaagac aggctgaccg ggagacgctt ggtggccttg gcctgcgcgg cggtgatgtc
     1201 cccctccttc tccatgcggc tcaagacgta gtcgcgtcgc tccttggctc gcttgaggtc
     1261 gttgaagggg gagtacaggc tcggggcttt gggcagacca gccagcatgg cggcctcggc
     1321 caagctgagc tggcccacgg atttgttgaa atacgtccgg gccgcagcct cgacgccata
     1381 cgctccgccg ccaaagtaga tcgtgttcag atacatctcc aggatcttgt ccttgctgta
     1441 gcgctgctcg atctcgcggg cgatctggat ctctttcagc ttgcgactca atgtccgttc
     1501 cggactgagg aagagactct tgctgagttg ctggg
//
LOCUS       DBNGZC010000548         2491 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2198802, whole genome shotgun sequence.
ACCESSION   DBNGZC010000548 DBNGZC010000000
VERSION     DBNGZC010000548.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2198802"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /locus_tag="AC16G0_09565"
     CDS             complement(<1..1309)
                     /locus_tag="AC16G0_09565"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5099897.1"
                     /translation="MPRWIAAGLMLICLATRAMAGEAPAGGPEAVLDGAAREAQAGNI
                     TVAIQSLQALAAAGVPATIQSRADLLLGMLLLKQDKREEAIPPLERAAATYPLLADYA
                     LYSLAAANRLAGRPAAAALALRRLLDGYPDSLFVERASREIVRDWLDAGELARVEETA
                     GRYLAAFPQASGRAGVWVALGEALIRSGRAEQAEEIFRRVWIELPGSPDAQRAKDLLA
                     TIPGARPFTPEEQFRRAMTAYQIGRYGQALHELDPFATAGDPHESQARLLLGISAFKL
                     RQYSLATKWLLPLRDAAGADRGEVAYWLGRSYGRAGDYPRFTEQMLLLADMAPQTRRG
                     EEALYLLAQAAANEGEVTQARTYLTRLVQEYPKGVWTDAGLWLQGWLAKKAGEPAAAL
                     AAWGRLLAEQPGSSLRAPALYWRGRVLEVKKHSTDAVKAYRTLL"
     gene            complement(1401..2453)
                     /gene="mtnA"
                     /locus_tag="AC16G0_09570"
     CDS             complement(1401..2453)
                     /gene="mtnA"
                     /locus_tag="AC16G0_09570"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HIR5099898.1"
                     /translation="MAEDFVTVAWTPSGTIRLIDQTRLPGEEVYVECRTIEEVADAIR
                     TMRVRGAPAIGVTAAMGMALGAQTISPTSFEDFMAGLERMGHLLSATRPTAVNLRWAV
                     QRILELARKSRDLSPAEIKAALVMEAQEMCQEDIRINRAMGRHGQTLVPSPARVLTHC
                     NAGGLATAGYGTAVGVIRAAAEAGKRVMVWVDETRPLLQGARLTAWELQKAGIPATLI
                     TDNMAGYFMGRGQVDLVVVGADRIARNGDVANKIGTYGVSLLARAHGIPFYVAAPIST
                     LDLSLIDGSGILIEERAIEEVTHIGGRRLAPAGIRVAHPAFDVTPASCVDAIITERGI
                     ARAPYDVTLPRLAGEV"
BASE COUNT          395 a          908 c          784 g          404 t
ORIGIN      
        1 ccagaagggt ccggtacgcc ttgaccgcgt ccgtcgaatg tttcttcacc tccagcaccc
       61 gtcctcgcca gtacagggcc ggcgcacgaa gggacgaacc gggctgctcg gccaggaggc
      121 gcccccacgc cgccagagcc gcagcgggct ctcccgcctt cttcgccagc cacccctgaa
      181 gccagagccc cgcgtccgtc cagacgccct tcgggtattc ttggaccagg cgggtcagat
      241 acgtccgggc ctgtgtgacc tctccctcgt tggccgccgc ctgtgccagg aggtagagtg
      301 cttcctctcc ccggcgggtc tggggtgcca tgtccgccag gagaagcatc tgctccgtga
      361 aacgcggata gtctccggcc cggccgtagc tacgcccgag ccagtaagcg acctcgcccc
      421 gatccgcccc cgccgcatcc cgcaagggca gcaaccattt ggttgctagg ctatactggc
      481 gcagtttgaa agcgctgatg cccaggagga ggcgggcctg gctctcgtgc ggatcaccgg
      541 ccgtcgcaaa gggatccaac tcgtgaagcg cctgcccgta ccgcccgatt tgataggccg
      601 tcatggctcg ccggaactgc tcttccggcg tgaagggccg ggcgccgggg atggttgcca
      661 ggaggtcctt cgcccgctgg gcgtccggac tgcccggcag ctcgatccag actcggcgga
      721 agatctcttc cgcctgctct gcccgtcccg atcgaatcag cgcctcgccc agcgccaccc
      781 acactcctgc ccgcccagag gcctgcggga aggccgccag gtagcgacct gccgtctcct
      841 ccacgcgcgc aagctctccc gcatccagcc aatcccgcac gatctcccgg ctggctcgct
      901 cgacgaacag gctgtccggg taaccgtcca gcagacgccg cagggcaagg gcggcagccg
      961 cgggtcggcc ggcaagccgg ttggccgcag ccagagaata gagggcgtag tccgcgagca
     1021 acggataggt cgccgccgcg cgctccagcg gggggatggc ctcttcccgc ttgtcctgct
     1081 tgagcaggag cattcccagt aggaggtcgg cccgcgactg aatcgtggcg ggaacccctg
     1141 ccgccgccag ggcctgcaag gattgtatcg cgaccgtgat gtttcccgcc tgcgcctccc
     1201 gggccgcccc atccaggacg gcctccggtc cgccggccgg agcctcgccg gccatggctc
     1261 gggtggcaag gcagatcagc atcaggccgg ccgcgatcca tcgaggcatc gtcagccttg
     1321 tggcgcaccg ccttgtcgcg tccaggatca taacacccct tgcccttggg atatgaacga
     1381 ggaaaatcac caggcgagcc tcacacctcg cctgccaggc gcgggagggt cacgtcgtac
     1441 ggggcacggg cgatgccgcg ctctgtgatg atcgcgtcca cacagctcgc cggagtgaca
     1501 tcgaaggcag ggtgggcgac ccggatacct gccggcgcaa gcctgcgccc cccgatgtgg
     1561 gtcacctcct cgatcgcccg ctcttcaatc agaatcccgg acccgtcgat caggctgagg
     1621 tccaaggttg agatcggtgc cgccacgtag aacgggatgc cgtgtgcccg ggccaggagg
     1681 gacacgccgt acgtcccgat cttgttggcg acatccccgt tccgggcgat gcggtccgcc
     1741 cccaccacca cgagatccac ctgtccccgc cccatgaagt aacccgccat gttgtcggtg
     1801 atgagcgtgg cagggatgcc tgccttttgc agctcccaag ccgtcagacg cgcaccctgg
     1861 agcagcggcc gcgtctcgtc cacccacacc atgacgcgct tacccgcctc ggcggcagcg
     1921 cggatgacgc cgacggcggt cccatacccc gccgtggcaa gtccccccgc attgcagtgt
     1981 gtgagcacgc gcgccggact tgggaccagg gtctgcccgt gccggcccat ggcacgattg
     2041 atccggatgt cctcctggca catctcctgg gcctccatca ccagggccgc cttgatctcc
     2101 gcaggagaga ggtcccgcga cttccgcgcc agctccagga tacgctgcac cgcccagcgg
     2161 aggttcaccg ccgtgggccg ggtggcggag agaaggtgtc ccatcctttc cagtcccgcc
     2221 atgaagtctt cgaagctggt cggcgagatc gtctgggcgc ccagggccat ccccatggcg
     2281 gcggtcacgc cgatggccgg ggcccctcgg acccgcattg tccggatggc gtccgccacc
     2341 tcctcgatag tccggcactc gacatagacc tcctctccag gcagcctggt ttgatcgatc
     2401 agtcgaatcg tgccggacgg ggtccaggca accgtcacga aatcctcggc cattccaaac
     2461 cctcgtgtga tgcgaagttg ctgggttagc c
//
LOCUS       DBNGZC010000549         2251 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2298017, whole genome shotgun sequence.
ACCESSION   DBNGZC010000549 DBNGZC010000000
VERSION     DBNGZC010000549.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2298017"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="AC16G0_09575"
     CDS             <1..864
                     /locus_tag="AC16G0_09575"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HIR5099899.1"
                     /translation="ALLTILLNAVLLRTAPGWISGRDRFGPAGTLSTPRRDDRRQGHV
                     ILCGFGALGSAVGEALETFRCPFLVIETDPDIIQGLASRGIPTLYGDAQQERILEDAG
                     LVRASALIVTLTEGAATYGIVRAARTLHREIPILVRAASPEEQQRLRTAGATGVVQPR
                     LEGAAFLIRETLAHLGLPSERTDAYLERFREVMDGPVRLGPSGPDVLPELREIQLTPA
                     GELADQSLRQARVRERFGVLVLAVKRADGTSMVNPSPEIPLRPGDRLRVFGLPAQLAA
                     FEAATGGGGTQ"
     gene            1366..>2251
                     /locus_tag="AC16G0_09580"
     CDS             1366..>2251
                     /locus_tag="AC16G0_09580"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR5099900.1"
                     /translation="MPILLKRLLVGTPLATAEAQHERLGKIAALAIFCSDAISSVAYG
                     PEEIRIFLALAGTAALSLSVPIAAAITFLIAVVSTSYWQTIHAYPAGGGAYTVARENL
                     GRFPGLVAGASLLIGYVVTVAVSVAAGVAALTSAFPSLYPARVWVGIGCVTFLAVVNL
                     RGLRETGRIFAVPTYWFIASIALAMGAGLAHLWRGDPLPDFPPLDPSTLAPLTTLLVL
                     RAFSSGCTAMTGIEAVANCVQAFKPPEAKNAGITLIWMASILGIMVMGLTYLSDAFRL
                     LPREGETLVSQLAGQTLGR"
BASE COUNT          361 a          748 c          727 g          415 t
ORIGIN      
        1 gcactgctga ccatcctgct caatgccgtg ctgctgcgga ccgcgcccgg gtggatctct
       61 ggccgggacc gcttcggccc ggccggcacg ctgagcacgc cccggcgtga cgatcggcgc
      121 cagggtcatg tcatcctgtg tggcttcggc gccctgggga gcgcggttgg agaggctctg
      181 gaaaccttca ggtgtccctt cctggtaatc gagaccgatc ccgacatcat ccaggggctt
      241 gccagtcggg ggattcccac cctttatggg gatgcccagc aggagcgcat cttggaggac
      301 gcgggcctcg tccgggcgtc cgcgctgatc gtcacactga cggaaggggc ggcgacctac
      361 gggattgtgc gggcggcacg gaccctgcac cgggagatcc cgatcttggt gcgggccgca
      421 tcgccggaag agcagcagcg gctgcggacg gccggggcca ccggcgtcgt ccagccccga
      481 ctggaagggg cggcatttct catccgggag acgctggcgc acctcgggtt gccatctgag
      541 cgcaccgacg cctacctgga gcggttccgc gaagtcatgg acggacccgt ccgcctgggc
      601 ccatcagggc ccgacgtctt gcccgagctt cgagagattc agctcacccc agcgggcgaa
      661 ctggcggacc aatcgctccg ccaagcccgt gtgcgggagc gcttcggcgt cctggtcctg
      721 gccgtgaagc gcgcggacgg gaccagcatg gtgaacccgt ctcctgaaat acctctgcgc
      781 cctggtgacc ggctccgcgt cttcgggctc cccgcacaac tcgcagcatt tgaggcggca
      841 accggcggtg gtgggaccca gtgaactcgg cccaaccggc ttctactatg cctctccggg
      901 tacacggacg gagccgcggg agcaacgtta gagagatgcg ggactcgtag gatcccaagt
      961 ctccgggtgg gctgcatggt ggcgcgatgc atgacgaaat cccgagaaac cgtgtcatct
     1021 cggaagacag cggtccgctc gatcccttca caatcatatc ggtagcgggc gtcggccgat
     1081 ggttcggtca cacagcgcgg gcacgcatcg acacacgcct cgcgagaggt ggaagcagag
     1141 tcgagcacca ggagtcataa gatgcaacgg atcccctaat gcaactctga gttgaatcga
     1201 caaggaaccc atggctgttt acaacctaca caacctccgc cgatagcttg acatacaacg
     1261 agcgcaagtc gtagagtcgc cccgtacact gttcccttta gtcggcggga ttcccaaggc
     1321 caggtcagtc gccgcggaac tggtatctgc tcacgttgtg gcccaatgcc catactcctc
     1381 aagaggttgc tggttggcac gcctttggcc acggccgagg cccagcatga gcggctcgga
     1441 aagatcgcgg ctctggccat cttctgctcc gacgcgatct cgtcagtggc ttacggcccg
     1501 gaggaaatcc ggatctttct ggcgctggcg gggacggcgg cgctgtccct gtccgtcccc
     1561 atcgcggcgg cgatcacctt cctcatcgcc gtggtctcga cctcctattg gcagacgatc
     1621 catgcctacc ccgccggtgg tggcgcgtat accgttgctc gggagaatct ggggaggttt
     1681 cccggactgg tggcgggagc ttcgctcctg atcggatatg tggtgaccgt cgccgtgagc
     1741 gtggccgccg gcgtggccgc cctcacctcc gctttcccga gtctgtatcc cgcgcgcgtc
     1801 tgggtgggga tcggatgcgt gaccttcctc gccgtcgtga acctccgcgg gctgcgggag
     1861 accgggcgaa tcttcgccgt tcccacctac tggttcatcg ccagcatcgc tctcgcgatg
     1921 ggggccgggc tggcccacct ttggagaggg gatccgctcc cggatttccc ccccctggac
     1981 ccgtccactc tggctcccct cacaaccctc ttggttctgc gcgccttcag ctccggctgc
     2041 accgcgatga ccggaatcga ggcggtcgca aactgcgtcc aggccttcaa gcctccggag
     2101 gccaagaacg caggcatcac cctgatctgg atggcatcca ttttggggat catggtgatg
     2161 gggctgacgt acctcagcga tgcctttcgc ctcctccccc gcgaggggga gacgctcgtc
     2221 tcccagttgg ctggtcagac cctgggccga g
//
LOCUS       DBNGZC010000550         2378 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2198927, whole genome shotgun sequence.
ACCESSION   DBNGZC010000550 DBNGZC010000000
VERSION     DBNGZC010000550.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2198927"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            664..>2378
                     /locus_tag="AC16G0_09585"
     CDS             664..>2378
                     /locus_tag="AC16G0_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HIR5099901.1"
                     /translation="MPVELSGQMRQFGYNVFRGPAGTFAPVENLPVGPDYVLGPGDDL
                     TIYIWGLVESYFTATVDRSGEILIPRVGPLKVWGLTFEKAQQLIRDKLSQVFTGFQVS
                     VTLGRLRTIQVFLLGEVGLPGAYNVNALSTLTHALFAAGGPTKQGTLRGIKLLRNNQQ
                     LAEFDFYDFLLRGDKSQDVRLEPGDTILVPPIGPVVGLVGDVKRPAIYEMKGGMLAAD
                     LLTLAGGVSPGGYLQRLQLERFRANTDRLVVDFNLLDFYRNGRQDSNPMLQDGDLVRV
                     FPVDPRIYNVVTLEGFVKRPGNYEYRPGMRVGDLLANDQLLPEAFLDRAELVRLRGDL
                     TTEVIPFNIREIQTGQGDSNLELRPRDRVVINSEFRAGASVLISGQVKRPGRYAINQG
                     ERLSSLLERAGGFLPGAFPKGAVFTRESIRQLERQQLDKFIQTQDQTLMAEVASGVEG
                     AAVLNVVRRELARSLAAVITLGRLSVQLDSPERIKAANDILLEDGDSLTIPQQPTSVF
                     VIGAVRNSASILFKEGENIEYYLSQAGGTRPEAEVKQTYILKADGTAVASFVKLRKLE
                     PGDAVV"
BASE COUNT          414 a          772 c          746 g          446 t
ORIGIN      
        1 ttgggccagg ctccatatgg ccaggcccca ttgggccagg ctccatatgg ccaagctcca
       61 tttgggcaga tacaatccct cccggcccct ccggcagtct ccgcgacacc gcgtcctacc
      121 ggtgcgccgg cggttccctc aggttcgagt gcctcgacct ttccctgtcc ggtcggattc
      181 actccggtac aatcggccca atcctatgtg aggtccttgg cgacgcagca gttccccagc
      241 gcgttcaccg gagggccgtt ctccacgcaa ggctcgacgc ctggggttcc ctctgggacc
      301 cagccaggct cgacgcttgg ggtctccccc ggaacccagc aaggttcggt gtccggagtc
      361 ccctccggca cccagcaagg ggtggctgcc ggtgccctcc tcccttccgc cgcgcagaac
      421 gtgctgaccg cccccgtctt cggaggccag ccagcgcccg aatcggctcc catctcccct
      481 tccatgacgc agggcgccac gcctggatcc ccctttggaa cgcaagcctt ggggccggtt
      541 gccgcggttc cctcgccgaa ccgaggtccc atcccggggc agttcccggc tcccgggttt
      601 cccggtatcg actgcattcc ttcggagccg ctctcgccca tcgaggctga ctttgagaaa
      661 cgagtgccgg tagaactttc cgggcagatg cgccagttcg gctacaatgt gttccggggg
      721 ccggcgggta ccttcgctcc cgtcgagaac ctcccggtag gacccgatta cgtgctgggg
      781 ccgggtgacg acctcacgat atatatctgg ggtctcgtcg agagttactt cacggcgacg
      841 gtcgatcgaa gcggagagat cctcattccg agagtcgggc cgctcaaggt gtgggggctc
      901 accttcgaga aggcgcagca actgattcgc gacaagctct cccaggtctt taccggcttc
      961 caggtcagcg tcaccttggg gcgtctgcgg accatccagg tgttcttgct gggagaggtg
     1021 ggattgcccg gggcatacaa cgtgaatgca ttgtccacgc tcacccatgc gctgttcgcc
     1081 gcgggcgggc cgaccaagca gggaacgttg cggggaatca agctccttcg caacaaccag
     1141 cagctagccg agttcgactt ctacgacttc ttgctgcgtg gggataagag tcaggacgtt
     1201 cgactggagc ccggcgacac tattctcgtt ccccccatcg gccccgtggt gggactcgtc
     1261 ggggatgtga aacggccggc catctacgag atgaaggggg ggatgctggc ggccgacctc
     1321 ctcacgctgg ccggcggggt ctctcccggg ggctacctgc aacgtctcca actggagcgc
     1381 ttccgggcga acaccgatcg cctggtcgtg gatttcaacc tgctggattt ctatcgcaac
     1441 gggcggcagg actcgaatcc gatgctgcag gacggcgacc tggtgcgggt cttcccggtg
     1501 gatccccgca tctacaacgt ggtgaccctt gagggcttcg tcaagcgccc cgggaactat
     1561 gaatatcgcc cggggatgcg cgtgggagat ctgctggcaa acgatcagct cctcccagaa
     1621 gcattcctcg atcgcgccga gctggtccgg ctgcggggag acctgaccac cgaagtcatt
     1681 cctttcaata ttcgagagat acagaccggc cagggagatt ccaacctcga actccgcccc
     1741 cgagatcggg tggtgatcaa cagcgagttc cgggcggggg cgtcggtcct catcagcggt
     1801 caggtgaagc ggccgggccg ctacgccatc aaccagggag agcgcctcag cagccttctg
     1861 gagcgagcgg gcggcttcct gcccggagca tttcccaagg gagccgtttt cacacgagag
     1921 tccattcggc agctcgagcg gcagcagctc gacaagttca tccagacgca ggaccagacc
     1981 ttgatggcgg aagtggcatc cggcgtggag ggggcggcgg tgctcaacgt cgtgcgccgc
     2041 gagcttgcca ggtcccttgc ggcggtcatc acccttggga gactctctgt tcaactggac
     2101 tcacccgaga ggatcaaggc ggcgaacgat atcctgctgg aagacgggga ttccctcacc
     2161 atcccgcaac agccgacctc cgtctttgtt atcggagcgg tccggaacag tgcctccatc
     2221 ctcttcaagg aaggtgagaa tatcgagtac tacctctcgc aggcaggggg gactcggccc
     2281 gaagctgagg tcaagcagac atacatcctc aaggcggacg gcactgccgt cgcgagcttc
     2341 gtgaagctcc ggaagttaga gccgggagat gcggtggt
//
LOCUS       DBNGZC010000551         3666 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_928024, whole genome shotgun sequence.
ACCESSION   DBNGZC010000551 DBNGZC010000000
VERSION     DBNGZC010000551.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_928024"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="AC16G0_09590"
     CDS             <1..418
                     /locus_tag="AC16G0_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5099902.1"
                     /translation="FLESQGGTVDVKLDDIPKDAGTVAGAGGGQAAELKLPGDPKAGQ
                     AIFMGKGACIGCHKAGAIGASPVGPDLSQIARIQTPEYIMAKILNPAAMGTVAGYPPN
                     VMPPMFGQTLTAREYIDLVSFLLTLKGEASASAAKP"
     gene            415..2652
                     /locus_tag="AC16G0_09595"
     CDS             415..2652
                     /locus_tag="AC16G0_09595"
                     /inference="COORDINATES: protein motif:HMM:NF021010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5099903.1"
                     /translation="MKILHSKFLQALLILVGTFLFFRFGIRPPAPWSVVRLYMTITVF
                     AVLVYVSSNSDSWESFLQPIRSTLADDSKRQIRLALMIIFPLLLGYYTYSKAGTELGA
                     PPELRAVHPAPPASMTFRGKTLNIQGLENPLRKDVANFKKYVAEGGIIYIRNCVYCHG
                     DNLDGNGHFAPAFNPRPANFTDPGTIAMLQEGYLFWRIAKGGPGLPKESAPWNSVMPA
                     WEDRLSEEEIWKVILFLYDDTGYQPRRWETQASLPSAPIRSDDQGDRWRGVFWKHAQA
                     EAQQAGDPASGKAVFEKKCALCHGAEGKGDGPGAATLDPKPRDFTKGLYKIRTTASGQ
                     PPTDTDLFRVITEGMIGTSMPGWKMLPEKDRRDLVAYLKTLATDKFKEPPKAPDFPKE
                     VASSKESLIRGKEMFEAIECNKCHGSAGRGDGPSAPELKDDWGNPIRPANLTKPWTFR
                     GGASTRDIATRLVTGLMGTPMPSFIDSVEKPEDIWHVANYVKSLGQENPNFATILAVH
                     PVQGEIPDDPASPFWAGQVPANFPLAGQVTVDPRNFTPSIDMITARAVYSDNEIAFHL
                     SWDDPTASKTNPESKTFADQVALQFPAKLSEENDRPYLLMGDGANSVFLVRWSSDAGV
                     EEATASGLGAITPRRKEETQAKGKAIYENGRYRLVVKRPLAAPAQAASPTLSIGTFIP
                     VAFFAWDGGQGEVGTKFSMSSWYYLRLEPPPSKARWMYPPLVVVAVFGLEVWGLRRFK
                     RRVHG"
     gene            2739..3104
                     /locus_tag="AC16G0_09600"
     CDS             2739..3104
                     /locus_tag="AC16G0_09600"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5099904.1"
                     /translation="MRLIAWWVVCVVVALSQLPGTALGAGDVAKGKAKYQEVCAACHG
                     ATGKGDGPAAAGLPTKPANHTDAAYMGKLKDQQIFDTIKKGGQAVGKSPLMPPWGGQL
                     SDQQIRDLVAYIRTLAKKP"
     gene            3143..3640
                     /locus_tag="AC16G0_09605"
     CDS             3143..3640
                     /locus_tag="AC16G0_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR5099905.1"
                     /translation="MAKLNINGRVRDVQVEPDTPLLWVIREQVGLTGTKYGCGIAQCG
                     ACSVHINGEVQRSCSIRVGDVKATDRIVTIEGLSASGSHPVQKAWAALDVPQCGYCQS
                     GQIMAAAALLRQNPKPTDKDIDEAMTNICRCGTYQRIRAAVHMAAGMARGGSSFAPAQ
                     GKSKV"
BASE COUNT          726 a         1147 c         1155 g          638 t
ORIGIN      
        1 gtttctggag tcacagggcg gcacagtgga cgtgaagctt gacgatatcc ccaaggacgc
       61 ggggacggtg gcgggtgccg ggggtggaca ggcggcggag ctgaagcttc cgggggatcc
      121 gaaggccgga caggctatct tcatggggaa gggggcctgc atcggctgcc acaaggcagg
      181 cgccatcggc gcctcgccgg tcgggcctga cctgtcccag atcgccagga tccagacacc
      241 cgagtacatc atggccaaga tcctgaaccc ggctgcgatg ggaaccgtgg cagggtatcc
      301 gcccaacgtg atgccgccca tgttcggtca aaccctcacc gccagggagt acattgatct
      361 cgtgtccttc ctcctcacgt tgaagggcga ggcctcagcc tccgcggcga agccatgaag
      421 atcctccact cgaagtttct ccaggccctc ctgatcctcg tggggacctt tctgttcttc
      481 cggtttggga tccggcctcc ggctccgtgg agtgtggtac gcctatatat gaccatcacc
      541 gtcttcgcgg tcctggtcta cgtctcgtcg aattccgatt cctgggaatc gtttctccag
      601 ccgattcggt caacactcgc ggatgactcc aagcgccaga ttcgtcttgc cctcatgatt
      661 atctttccgc tcttgctggg ctactacact tacagcaagg ccgggacgga gctgggggcg
      721 cccccggagc ttcgggcggt ccacccggct cctcccgcct ccatgacatt tcgggggaag
      781 acactaaata tacaggggct cgagaatccc ctccggaagg acgtggcgaa cttcaagaag
      841 tatgtggcag aaggcggaat catctacatc cggaactgcg tctactgtca cggagataat
      901 ctcgacggca acggccactt cgcccccgcc ttcaacccgc gcccggcgaa cttcacggac
      961 cccggcacca tcgccatgct gcaagagggc tatctcttct ggcggatcgc caagggcggt
     1021 cccggtctgc cgaaggagtc cgccccgtgg aactcggtca tgccggcctg ggaggatcgg
     1081 ctcagtgagg aagagatctg gaaggtgatc ctgtttctct acgatgacac gggctaccag
     1141 ccgagacgct gggagacgca ggcgtcgctc ccatccgctc ccatcaggag cgatgaccag
     1201 ggtgaccgat ggcgcggagt attctggaag cacgctcagg cagaggcgca gcaggccggt
     1261 gaccccgcca gcgggaaggc cgtgttcgag aagaagtgcg ccctgtgtca cggcgcggaa
     1321 gggaaagggg acggccccgg ggccgccacc ctcgatccca agccgcggga tttcaccaag
     1381 ggtctgtaca agatccgtac aactgcttcc ggtcagccgc ccacggacac ggaccttttc
     1441 cgagtcatca cggagggcat gatcggaacc tccatgcccg gctggaagat gctcccagag
     1501 aaggaccgac gggacctggt cgcgtacctc aagacccttg ccactgacaa gttcaaggag
     1561 ccacccaagg cacccgactt ccccaaggag gttgcgtctt ccaaggaatc cctcatccgt
     1621 ggaaaagaga tgttcgaggc catcgagtgc aacaagtgcc acggcagcgc ggggcggggt
     1681 gacggccctt cggcacctga actcaaggat gactggggaa accccatccg gccggccaat
     1741 ctgaccaagc cctggacctt ccgaggggga gcgagcacca gggacatcgc cacccgcctg
     1801 gtgaccggcc tcatgggaac tcccatgccg tcattcattg acagcgtgga gaagcccgaa
     1861 gacatctggc acgtggccaa ctatgtgaaa tccctcgggc aggaaaaccc caacttcgcc
     1921 acgatcctgg cggtgcatcc agtgcagggg gagatccccg acgatcccgc ttcgccattc
     1981 tgggccgggc aggttcccgc aaattttccg ctggccggac aggtgaccgt ggatccccgg
     2041 aacttcaccc cgtccatcga tatgatcacg gcgcgagcgg tttattctga caatgagatc
     2101 gcctttcacc tgagctggga tgaccccacc gcctccaaga cgaatcccga aagcaagacg
     2161 ttcgccgacc aggtcgccct tcagttcccg gccaaactct ccgaggagaa cgaccgccct
     2221 tacctgctca tgggcgatgg agcgaattcc gtcttcctcg tacgctggtc ctcggatgcc
     2281 ggggtggagg aggcgaccgc gagtggcctg ggggcgatca ctccccggag gaaagaggaa
     2341 acccaggcga aggggaaggc catatacgag aacggccgct accgcctggt cgtgaagcgg
     2401 cccctggcag cgcccgcgca ggccgcttcg cccacgctct caatagggac gttcatccct
     2461 gtcgcttttt tcgcctggga tggaggtcag ggtgaggtcg gaaccaaatt ctcgatgtcg
     2521 tcctggtact atctgcgctt ggagccgccg ccatccaagg cccgctggat gtatccgccc
     2581 ttggtggtgg ttgccgtctt tggactcgag gtctgggggc tgcggcgatt caagcgtcgt
     2641 gtgcacggct gatggccaag tgtggccccc ccagactcaa tggactcagc aggctcactg
     2701 aactcgaagg attcaacaaa ctcaagggag gaggagaaat gcgtctgatc gcatggtggg
     2761 tagtttgcgt ggttgtggct ctctctcagc ttccgggaac tgcattggga gccggtgatg
     2821 tggcgaaggg aaaggcgaag taccaggagg tgtgcgcggc ctgccatggt gcgacgggca
     2881 aaggggatgg accggcggcg gccggcctgc cgaccaagcc cgccaaccat accgacgcgg
     2941 cctacatggg gaagctcaag gatcagcaga tcttcgatac catcaagaag ggcggccagg
     3001 ccgtggggaa atcgcccctc atgcccccct ggggcggcca gctcagtgac cagcagatcc
     3061 gggatctcgt ggcctacatc cggacactgg ccaagaagcc ctgagcaggc ctgatctgga
     3121 cgctgcatgc gggaggatgt ccatggcaaa gctcaacatc aatggcagag tccgtgacgt
     3181 gcaagtggag ccagacacgc cgctcttgtg ggtcatccgc gagcaggtgg gcctgacggg
     3241 gaccaagtat ggctgcggaa tcgcgcaatg cggcgcctgc tccgtgcaca tcaacggcga
     3301 ggtgcaacgc tcctgctcca tccgcgtcgg agacgtgaaa gccaccgaca ggatcgtgac
     3361 catcgaaggc ctctcggcct ccggctcgca tcccgtgcag aaggcctggg cggcgctcga
     3421 cgtaccccag tgcggctact gccagtcggg gcagatcatg gctgccgcgg cgctgctcag
     3481 gcagaaccca aagccgaccg acaaggacat cgacgaagcg atgaccaata tctgtcggtg
     3541 cggcacctat cagcgcatcc gggcggccgt gcacatggcg gctggcatgg ctcgaggcgg
     3601 cagctcgttt gctcccgctc agggcaagtc caaagtctag caggctgctg acgaacgcat
     3661 tcttgc
//
LOCUS       DBNGZC010000552         8850 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1514858, whole genome shotgun sequence.
ACCESSION   DBNGZC010000552 DBNGZC010000000
VERSION     DBNGZC010000552.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 8850)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8850)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8850
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1514858"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(355..1065)
                     /locus_tag="AC16G0_09610"
     CDS             complement(355..1065)
                     /locus_tag="AC16G0_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HIR5099906.1"
                     /translation="MESNADMKLDVLEHGANQQKCDRRLFVQLQVFSWCSETKPLIAA
                     LEASQVEAVLYLDISDPTGVGVLSMSEDPTFFVTRLRELLGSPPFAGLQRRPDLAMLG
                     RSYSSGFEPDLEEYLLRRPRRTARNPDWPWAVWYPLRRTGAFARLSPKEQGAILREHG
                     TIGHAYGDAGLAHDIRLACNGLDVNDNDFVIGLVGPDLYPLSHLVQQMRRTIQTSEYI
                     EKLGPFFVGRACWQSPLR"
     gene            complement(1196..2596)
                     /gene="asnS"
                     /locus_tag="AC16G0_09615"
     CDS             complement(1196..2596)
                     /gene="asnS"
                     /locus_tag="AC16G0_09615"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005346717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HIR5099907.1"
                     /translation="MPWLKVVDALKVPVGTECTVKGWVRTRRDSKADGGLSFITVHDG
                     TCQDPVQVVVRSSLANYQSDVQRLSTGCAIEVDGTLSASQGKGQAVELLASAVRVVGW
                     VDDPDSYPVSAKRHTFEYLREVAHLRVRTNTFGAVARVRHTLAAALHRFFDERDYYYV
                     HTPIITTSDAEGAGQMFRVSTLDLVNLPRTPAGGVDFSQDFFGREARLTVSGQLNVES
                     YCCALTRVYTFGPTFRAENSNTSRHLSEFWMIEPEIAFAGLDENADLAEAMLKYLFDD
                     LLRRRADDMAFFDLRIEPGVVDRLRHVVETPFRRLTYTEAVEVLKKSGRTFEFPVEWG
                     TDLQSEHERYLTEECFKQPVILVDYPKEIKAFYMRLNDDGKTVAAMDVLVPKIGEIIG
                     GSQREERLEVLDRRIQEAGLRKEDYWWYRDLRRYGTVPHAGFGLGFERLIQFCTGMGN
                     IRDVIPFPRAPRQAEF"
     gene            3117..3569
                     /locus_tag="AC16G0_09620"
     CDS             3117..3569
                     /locus_tag="AC16G0_09620"
                     /inference="COORDINATES: protein motif:HMM:NF039485.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HIR5099908.1"
                     /translation="MCRPIGSGAHVRKGDVMGLLDGLLQGLSGGQGDSGGGGLQQALF
                     QMLQQGGTEGLSGLAQNFQSQGLGDIFSSWVGTGENKPIQPEQLQNALGAGTLQQLTS
                     GTGTSLQAALPLLAQLLPQVIDKLTPQGQLPQNNNLLQQGLSALFGQK"
     gene            complement(3948..4247)
                     /locus_tag="AC16G0_09625"
     CDS             complement(3948..4247)
                     /locus_tag="AC16G0_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099909.1"
                     /translation="MKRFVLLAAILLFAVSVAFAASVKTYQVTGPVLEVKDDMIVVQK
                     GNDKWEIARDKDTKVTGDLKVGSKVTIQYTMTAKEIESKEAPAKKEPAKKAPAKK"
     gene            complement(4409..5992)
                     /locus_tag="AC16G0_09630"
     CDS             complement(4409..5992)
                     /locus_tag="AC16G0_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008174578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR5099910.1"
                     /translation="MLNTPRATRGMVVAPHHLAAQAGLAVLREGGNAVEAALAAASTV
                     AVVYPHMNSLGGDNFWLLAEPGREPVAILACGSAGKRATADLYRGCEAIPPRGPLAAN
                     TVAGVVSGWQAAFEIGARWGGRLPLGRVLADAIQYARSGVPVTNSQHRFTLAKLRELK
                     DVPGFAGQFLPTGEPPPPGALFRQPRLADTLRRLAQTGLEDFYRGEIGAAIAAELARI
                     GSPVTGPDLASHRAVETAPLRASLSCGTVFNTPPPTQGLASLIILGVFEHLRCTEAES
                     FPYLHGLVESTKQAFLVRDRIVTDPAYMQEDPAGFLTESALAEMARRIQPEKAAPWPA
                     PAQGGDTVWLGVIDGEGRAVSLIQSIYWEFGSGVVLADTGILWQNSGVSFSLTEGAVN
                     VLRPGRKPFHTLNPAMARLKDGRLMVYGTMGGEGQPQTQAAVFTRHVLYGQELQAAVT
                     APRWLLGRTWGATSTNLKLESRFDPSLIAALRNAGHEVEVVASFTDMMGHAGGIVRHP
                     SGVLEGAADPRGDGIAAAF"
     gene            complement(6039..8162)
                     /locus_tag="AC16G0_09635"
     CDS             complement(6039..8162)
                     /locus_tag="AC16G0_09635"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF023418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase TgpA family protein"
                     /protein_id="HIR5099911.1"
                     /translation="MPFPLAFKLNLYLLVLDAFGALYLTQILGWPEYATVLLLGLGSW
                     WADRLRVSVLHYRQLWDAVTLVFLAFAVLDLLFLAESFMVGVIHLLLFLLTYKLYNTR
                     THRDVLDIFILTFLQLVAASTLTVSFGFLLVFCLYMILGTWGVILFHLKREADLALPE
                     QSRELLTRPDLIRPGFLLCSMGVAIASLGLTLAIFLAIPRVGRTYLAMKAQFGTLVTG
                     FTDRVDLGAYGSIQNDPTIVMRISFSGGTGSPDRNPNLLWRGLAFDRFDGQAWSVGDP
                     GRTPIRGARDGYFPVHPIRPGGPILTAEIFLEPIGTEVIFGLPRVIGIHGPFPSLSLD
                     TGGGLNLQAPPGSRIRYEVVSQPERFADAQLRRPARPEDFPLEIRRMYLQLPELSPRL
                     RALAQELAAGAMTPYETARRVETYLNENLRYSLDLRRESDLDPLDAFLFQRKAGNCEY
                     FAASMAILLRAAGIPTRVVNGFQRGEWNEVGRYFAVRQRDAHSWVEVYLPQAGWVTFD
                     PTPRAAFEAQAFGESSWAGKYLDALRMRWNRYVIDYSLGDQVLIAMSLRSQSRLFRRS
                     LGQTLELWSFQIGRTLRRLWRNYGYLVGVLLGLLVAGGVLFRRAAMAGVGLDRLPWRR
                     ATRTSVIFYERMIRLLARRGYARAPTATAREYASSLADRPHVYATVAELTALYEGIRF
                     GGEALTASDKSRAAALLRQLAEAHR"
     gene            complement(8165..>8850)
                     /locus_tag="AC16G0_09640"
     CDS             complement(8165..>8850)
                     /locus_tag="AC16G0_09640"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR5099912.1"
                     /translation="HHFLLRLAPQAREVWQYRLGFPRRGRHGLPGVQVLTRFPFGLFA
                     KISRPMLRDPLLVYPAVRALAPDELPAALGPGWHERERRGQGAGLYNLRPYRPGDDPR
                     MLHWKTSAKAGELMLKESEDEDRPHVRLVVADPAPQTPPLVVEANLSYAASLAAHAIR
                     LACQVQLVTADGSTEYGQGETHLDRILERLALYEVPASPRPVPISAGSGRTVQIHLDA
                     RRSTADAGA"
BASE COUNT         1603 a         2905 c         2874 g         1468 t
ORIGIN      
        1 tccggagacc gccgcggcca gcccgatctt gatgggctcc ttggattgcg cgtgtgcggg
       61 ctggcttgca gcgaggcaga cccctagcag gatgaagagc aacatcgtta cgcgacccat
      121 cgaagccacc ttggcggacg ggaatccgca tgtagggacg gacaacatca ctgtcagctc
      181 ggatgtctgg cagttggact ggcgcggcag gacgcgtact tttaccacaa ttgggggtga
      241 aagaaaacga cccctcaccg attgccaccg gcggccaact gggcatccag agacttcgtc
      301 gggtgtcacc cgaccgcgcc ggtgggcatg agggttgtca ggcggtgtga gcctttagcg
      361 gagggggctt tgccagcagg ctcggccgac gaagaacggg ccgagtttct cgatgtactc
      421 cgacgtctgg atcgtcctgc gcatctgctg gacgagatgc gagaggggat agagatcagg
      481 gccgaccagc ccgatgacga agtcgttatc gttgacatcc aggccgttgc aggccaagcg
      541 aatgtcgtgg gcaagcccgg catccccgta ggcatgcccg atggtaccgt gctcccgcag
      601 gatggccccc tgctctttcg gcgacaggcg ggcgaacgcc ccggtccgcc gcagtggata
      661 ccacaccgcc cacggccagt cggggtttcg cgccgtgcgg cgaggccgac ggagaagata
      721 ctcttccagg tccggctcga agccggagga ataactccgg ccgagcatgg cgagatccgg
      781 ccgacgctgg aggcccgcaa acggcggtga gccgagcagc tcgcggaggc gcgtcacgaa
      841 aaatgtcgga tcctcgctca tggacagcac gccgacgcct gtcggatcgc tgatatccag
      901 atacagcacc gcctcgacct gactcgcttc cagggcggcg atcagcggtt tcgtctccga
      961 gcaccagctg aacacctgta gctgcacgaa cagacgtcga tcacacttct gctggttcgc
     1021 cccatgctcc aggacgtcca gcttcatgtc cgcattggat tccacgcgca ccccccttgt
     1081 ccgtgacccg tcccccgctc tacctcgcgt ccccacaccc gtccgtcatc gttatcccag
     1141 aggatccccg tcccttcgac cctccactcg tcttgacgag ccgccccgtg cctcctcaga
     1201 actccgcctg gcgcggtgcg cgggggaacg ggatgacgtc gcggatgttt cccatgccgg
     1261 tgcagaactg gatcagccgc tcgaagccga ggccgaaccc ggcgtggggg acagttccgt
     1321 agcgacggag gtcgcggtac caccaataat cctctttccg caagcccgcc tcctggatgc
     1381 gtcggtccag gacctccagg cgctcctcgc gctgcgagcc gccgatgatc tcaccgatct
     1441 tcggcaccag cacgtccatg gccgcgaccg tcttgccatc gtcgttcagg cgcatgtaga
     1501 aggccttgat ctcctttggg taatccacca ggataactgg ctgcttgaaa cactcctccg
     1561 tcaagtaccg ctcgtgttcg ctctggaggt cggtccccca ctccaccgga aactcgaagg
     1621 tcctcccgga cttcttcagc acctccacgg cctcggtgta cgtcagccgg cggaacgggg
     1681 tctccaccac atgccggagg cggtccacca cccccggctc gatcctgagg tcgaagaaag
     1741 ccatgtcgtc ggcccgccgc cggagcagat cgtcgaagag gtatttcaac atcgcttcgg
     1801 ccagatcggc gttctcatcc agcccggcga aggcgatctc aggctcgatc atccagaact
     1861 cggacaggtg gcggctcgtg ttgctgttct ccgcccggaa ggtggggccg aaggtgtaca
     1921 cccgcgtgag ggcgcagcag tagctctcca cgttgagctg ccccgagacc gtcagccgcg
     1981 cctcgcggcc gaagaagtcc tgcgaaaagt ccacgccgcc ggctggcgtg cgggggaggt
     2041 tcaccaagtc cagcgtgctc acacggaaca tctggccggc cccttcggcg tccgatgtcg
     2101 tgataatggg ggtgtgcacg tagtagtagt cgcgctcgtc gaagaaccgg tggagggccg
     2161 ccgccagtgt gtgacgcacc cgcgcaacgg caccgaaggt attcgtgcgg acgcggagat
     2221 gggccacttc ccgcaggtac tcaaaggtgt gacgcttcgc gctcaccggg tagctgtcgg
     2281 ggtcgtccac ccatccgacc acgcggaccg ccgaggcgag aagctccacg gcttggcctt
     2341 tgccctgcga cgcactcagc gtgccgtcca cctcgatcgc gcagccggtt gagagacgct
     2401 gaacatccga ctggtaattc gccaggctgc tccggaccac cacctgcacg ggatcttgac
     2461 aggtaccatc gtgcaccgtg atgaaggaca gcccgccatc cgccttggaa tcccgccgtg
     2521 tccgcaccca acccttgacg gtgcactccg tcccgactgg taccttgagg gcgtctacca
     2581 ctttcagcca gggcatgacc gacctcctgt gtttggcggc gggaaaaagg atagtggagg
     2641 tgtccgaggc atgcaagaca tatccggcgg ccagtcccga acctcccttt ccgaccatgg
     2701 ccgctcggct gagattcccc ccggctcata cgagacctgc gcgacgaagc cttcatcgat
     2761 cagtccgagg cgatcgagat gatccagact cgccgcaggt tatgggcgac ccaaccccgt
     2821 agcccgcctg ccgcttccat tgccgcgtat tcagaggatg ggttggggtt ccctggactc
     2881 acgctcgatc ggccgctggg agtcgcggcc ctgagggccg tggggtccat ccagtcgcaa
     2941 cccacgttta gccttgacac tgagcgaggg tcggccggat actcccctcg acctggcgac
     3001 gcacccccgc tgcgtcggag tggtcgccca acgcggcgtt cagtccaaac gcccacgcgg
     3061 ccccgacaag ctgaattctt ccacctgttg gcattgaaac gtcctgttcg ggtgggatgt
     3121 gtcggcctat cggaagtggt gctcacgtgc gaaaggggga cgtcatggga ttgctggatg
     3181 gtttgctgca aggactttcg ggtgggcaag gcgactccgg cggcggggga ctccagcagg
     3241 cattgttcca gatgctgcaa caggggggca cagagggact ctcgggtctg gcccagaatt
     3301 tccagtccca agggctcggc gacatcttct cctcctgggt gggcaccggg gagaacaagc
     3361 ccatccagcc ggagcagctc cagaatgccc tgggtgcagg cacactgcag caactaacga
     3421 gtggcaccgg cacatcgctc caggccgccc tgccgctgct tgcacagctt ctcccccagg
     3481 tgattgataa gctcacaccg caggggcaat tgccgcagaa caacaacctg cttcagcagg
     3541 gtttgagcgc gcttttcgga cagaaatgac ggcaaccccg accgcggggc catccagaat
     3601 gccgcggaca ctccgcggaa tcatccgacc gggcgaagca tggtcaacgc gggcgggcgt
     3661 ccctccaggc ggtaggaaga actgatcgag aatctgatca tcaccaagga gaagtgcaag
     3721 gaagagagga aaggatcgtc gctgattatt tgctggcctt gatggtcgtt tcaggggggg
     3781 cgcgggcact tgcttgcgcc ctctgacttt tccagcagat ccgtatcgat ccggccaaag
     3841 gtggcggctc catcgacttt cgtgccgcgc tcgttggcct ccagaaaagc caggagcggc
     3901 aaccgttgag ctggctgccg ctccggtcgg cgagaatcgg tgatccccta cttcttcgcg
     3961 ggtgccttct tggcgggctc tttcttcgca ggcgcctcct tcgattcgat ctccttggcg
     4021 gtcatcgtat actgaatggt caccttggat cccaccttca ggtcgccggt gaccttcgtg
     4081 tccttgtcac gggcgatctc ccacttatcg ttgcctttct gcacaacgat catatcgtcc
     4141 ttgacctcga gaaccgggcc ggtaacctgg taggtcttca cggaggctgc aaaagcgacc
     4201 gagacggcga acagaaggat ggcggcaagc aacacgaatc gtttcatcgg ctcctccttt
     4261 cacggttgtg agggccccct ccagtattga acgcgcggcg atcttaaccg cagtccggga
     4321 aagccgtcaa cgggaatcgc gcaaggctgg tgcaggcaga ggccgaggct ccacgcgctg
     4381 ggatggccct ccgggcagga gggcgggatt agaacgccgc cgcgatgccg tcgccgcgcg
     4441 gatcggcggc accttcaagg acacccgacg ggtgtcggac gatgccgccg gcgtggccca
     4501 tcatatcggt gaacgacgcc acgacctcga cctcgtggcc ggcgttccgt agcgccgcaa
     4561 tcaatgaggg gtcgaaccgg ctctccagtt tcagattcgt gctggtcgct ccccaggttc
     4621 ggccgaggag ccagcgcggc gccgtcacgg ctgcctgcaa ctcctgccca tacaggacgt
     4681 gccgagtgaa gacggccgcc tgggtttgtg gctgtccctc gcctcccatc gtgccgtaca
     4741 ccatgagacg gccatccttg agtcgggcca tggcgggatt caaggtgtgg aacggcttcc
     4801 gtccgggacg caacacgttc acggcgcctt ccgtcagcga gaagctcaca ccgctgttct
     4861 gccacaggat gcccgtgtcg gcaaggacta cgccggagcc gaactcccag tagatgctct
     4921 ggatcaggct gacggcgcgc ccttccccgt caatgacccc gagccacacc gtgtcccccc
     4981 cctgcgcggg tgccggccaa ggcgcggcct tctccggctg aatccgccga gccatctccg
     5041 ccagggcgga ttcggtcagg aaccctgctg gatcttcctg catgtacgca ggatctgtga
     5101 cgatccggtc gcgcaccagg aaggcctgct tggtggattc gacgagaccg tggaggtagg
     5161 gaaagctttc cgcttcggtg catctcaggt gctcgaagac gccaaggata atgagcgagg
     5221 cgagtccttg agtcggcggg ggcgtgttga acaccgtccc gcagctcaag gacgcgcgaa
     5281 gcggcgccgt ctcgaccgcg cgatgcgatg caagatccgg ccccgtcacc gggctcccga
     5341 tccgcgccaa ttccgcggcg atggctgccc cgatctcgcc ccgatagaaa tcctcgaggc
     5401 cggtttgtgc cagtcttcgc aaggtgtcgg ccagtctggg ttgccggaac agcgcgcccg
     5461 ggggtggggg ctcgcccgtt ggcaggaact gcccggcaaa ccccgggaca tctttcagct
     5521 cccgtagctt cgccagcgtg aatcgatgct ggctgttcgt tacgggcacg ccgctccgcg
     5581 catactgaat cgcatccgcc aggactcgac cgaggggcag ccggccgccc cagcgcgccc
     5641 cgatctcgaa cgccgcctgc catcccgata ccacgcccgc cactgtgttg gccgccagcg
     5701 gcccgcgggg cggaatggcc tcgcagccgc gatagagatc tgccgtcgcc cgcttcccgg
     5761 cggacccaca ggcgaggatc gccaccggct ctcgtcccgg ctccgccagc agccagaagt
     5821 tgtccccccc taggctgttc atgtgcggat agaccactgc cacggtggag gccgccgcca
     5881 gcgccgcctc aacggcattc ccgccctcgc ggagaaccgc gagaccggct tgcgcggcga
     5941 ggtggtgcgg ggccacgacc atcccccgag tcgccctggg cgtattgagc atgagacatc
     6001 ctttccggaa tgccgccgag catcccagcg cttcgaggtc agcgatgggc ctctgcgagt
     6061 tggcgcagga gggcggcggc gcgagacttg tcgctggcgg tcaaggcctc gccgccgaaa
     6121 cggatgccct cgtacagggc ggtcagttcc gcgacggtcg catacacatg cggccgatcg
     6181 gccaggctgg aagcgtattc tctggcggtg gccgtcgggg ctcgcgcata gccgcgccgg
     6241 gccaggagtc ggatcatccg ttcgtaaaag atgacgctgg tacgagtggc gcgccgccag
     6301 ggaagcctgt ccagacctac cccggccatc gcggcccgtc ggaagaggac cccacctgcc
     6361 accaggagcc ccagaagaac gccgaccaga tatccgtaat tgcgccagag ccgccggaga
     6421 gtgcggccga tctgaaagga ccagagttcc agggtctggc ccaggctccg gcggaacaac
     6481 cgggactggc tgcgcaggct catcgcaatc aagacctgat caccgaggct atagtcgatc
     6541 acgtagcggt tccatcgcat ccgcagggca tcgaggtact tcccggccca gctactctcc
     6601 ccgaaggcct gggcctcgaa ggcggcccgc ggcgtggggt cgaaggttac ccaacctgcc
     6661 tggggaagat agacctccac ccaggagtgg gcatcgcgct ggcgcacggc gaagtaccgg
     6721 cccacctcgt tccactcccc tcgctgaaac ccgttcacca ctcgcgtcgg gatcccggcg
     6781 gcccggagga ggatcgccat gctggcggcg aagtactcgc agtttcccgc cttccgctgg
     6841 aacaggaacg catccagggg atccagatcg gactcccggc gcaggtccag gctgtagcgg
     6901 aggttctcgt tcaggtaggt ctccacgcgc cgtgccgtct cgtatggggt catggcgccc
     6961 gccgccagtt cctgcgccag cgcgcgcagg cgtggagaca gttccggcag ttgaaggtac
     7021 atccgccgga tctccaaggg gaaatcctcc gggcgagccg gccgccggag ttgcgcgtcg
     7081 gcgaaacgct ccggctggga caccacctcg tagcggatcc gcgagccggg cggggcctga
     7141 aggttgaggc ctccgccggt atccagtgac aggctgggga aggggccatg gatgccgatg
     7201 acccgcggaa ggccgaagat gacctcggtg ccgatgggct ccaggaagat ctcggcagtc
     7261 agaatcgggc ctcccggacg gatagggtga acggggaagt agccgtcgcg tgcgccgcgg
     7321 atgggggtcc gcccggggtc acccaccgac caggcctgtc catcgaaccg atcgaaggcc
     7381 agcccccgcc aaaggaggtt gggattgcga tccgggcttc ccgtgccccc cgagaagctg
     7441 atccgcatca cgatggtcgg atcgttctgg atgctcccat acgcccccag atccacgcga
     7501 tcggtgaacc ccgtgaccag cgtgccgaac tgcgccttca tggcgagata ggtccgcccg
     7561 acgcgaggga tggcaaggaa gatggccagg gtgaggccca gggacgcgat cgccacgccc
     7621 atgctgcaaa ggaggaaacc gggccggatg aggtccgggc gggtcagtaa ctcccggctt
     7681 tgctccggga gcgcgagatc ggcctcgcgc ttcaggtgga acaggatgac gccccaggtt
     7741 cccaagatca tgtacaggca gaaaaccagg aggaagccaa aactcaccgt gagggtggag
     7801 gcagctacga gctgcaggaa cgtcaggatg aagatgtcca ggacgtcgcg gtgcgtccgg
     7861 gtgttgtaca gcttgtaggt cagcaagaag aggagcagat ggatgacgcc gaccatgaag
     7921 gattccgcca ggaacaacag gtctaggacc gcgaacgcca ggaagaccag cgtgaccgca
     7981 tcccagagct gccgataatg aaggacggat acgcggagcc gatcggccca ccagctcccg
     8041 aggccgagga ggaggacggt cgcgtactcc ggccacccga ggatctgggt gagatagagc
     8101 gcgccgaagg cgtcgaggac caaaaggtac aggttgagct tgaaggccag cgggaagggc
     8161 acgtctaggc tcctgcgtcc gccgtggagc gccgggcgtc gagatggatc tggaccgtcc
     8221 gtcccgatcc cgcggagatc ggcaccgggc gcggcgacgc agggacctcg tagagcgcca
     8281 atcgctccag gatgcgatcc aggtgcgtct caccctggcc gtactccgtg gatccatccg
     8341 cggtcacgag ctgcacctgg cacgccaggc ggatggcgtg ggcagccagc gaggccgcat
     8401 agctcaggtt cgcctccacc acgaggggcg gagtctgcgg cgccgggtcg gcgaccacca
     8461 gcctgacatg ggggcgatcc tcatcctccg attccttgag catgagctcg ccggctttcg
     8521 ccgacgtctt ccagtggagc atccgaggat catctcccgg ccggtacggg cgcaggttgt
     8581 aaagcccggc gccttggccc cggcgctccc gctcgtgcca gccgggcccc agggcggcgg
     8641 ggagttcgtc cggtgcgagc gctcggacgg cggggtagac caggagcgga tcgcgcagca
     8701 taggacggct gatcttcgcg aagaggccga aggggaatcg ggtgaggacc tggacccccg
     8761 gaagtccatg ccgaccacga cgggggaacc ccagcctata ctgccagacc tcacgcgcct
     8821 ggggggcaag cctgagcagg aagtggtggg
//
LOCUS       DBNGZC010000553         3057 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1514989, whole genome shotgun sequence.
ACCESSION   DBNGZC010000553 DBNGZC010000000
VERSION     DBNGZC010000553.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1514989"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="AC16G0_09645"
     CDS             <1..1325
                     /locus_tag="AC16G0_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099913.1"
                     /translation="FKLRRGVKFHDGTPMNARTVAEDLDRAINPKNPCYVLDRKVDTY
                     DTFTFGSVKAGDAVKMDVLDDYTLRFSLPKPNAPFLSSLAMVWTGIMSPAATKQYNCD
                     AGQHPVGTGPFKFVEAVRNDHITVDANPEYWGGKPKVDRIIFRIIPESATRVLALERN
                     EVQILADVLPADYARIEKNSALKLYRQPGLTILGVAMSNDLGPFKDKRVRQAMNYAVD
                     KEAINKGLYGGATTASQGMPPVLWGFAKNVQPYPYDVNKAKALLKEAGYPNGFSTEMM
                     VSANPRGYNPIGGAKLGEAVQGYLAKVGVNVKISQYEWGAYLDRFRHTPWEGFAIAGW
                     SGDNGDPDNFLGDLFEWDTPANKARVNNTGRHHNPEYDKLMVQGRETTDQAKRVQIYE
                     KANQILHDDAAWIFINHTNQVRAARANVKGFQLNPLQMFFHMEQVSIE"
     gene            1420..2424
                     /locus_tag="AC16G0_09650"
     CDS             1420..2424
                     /locus_tag="AC16G0_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015390499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099914.1"
                     /translation="MWKYAARRLVQMLPVLLGVSLLVFLGMHLIPGDVAQLLLGDKGS
                     AEQLERLRHQLGLDQPIYVQYLRFLAEALRGDFGDSIRTRQPVIWEIGQALPVTIQLS
                     LAALAFAVVVGLVIGVVAARRPHSSLDTGTMVFVLIGVSMPVFWTGILLLLVFGGILG
                     WLPLGGVLDAGMSLRRVTGMPVVDALVSGNWAAARSGFAHLILPAVTLGATVMATIAR
                     MARSSMLDVLNLDYVRTARAKGVAERRVVRRHALWNALLPVVTLIGLQLGLLLSGAVL
                     TETIFGLPGLGRLTITSVLARDYPVVQGVVVIGAVIFVLANLVVDLLYAWLDPRIRYS
                     "
     gene            2394..>3057
                     /locus_tag="AC16G0_09655"
     CDS             2394..>3057
                     /locus_tag="AC16G0_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099915.1"
                     /translation="MARSADSLQLSAAGSPFAAEGRAATLSWRRLRRSPNLVAGAAIL
                     LLVLLSALLAPQVSPYDPIDQEFTSQLQPPSASHLFGTDEFGRDIFSRVIFGARIALS
                     VGGLADAIATALGVLLGVMAGYFGGRLDAVVTRVIDVLLAFPYLLLAMIVVAILGPGL
                     TNAMVAIAIVYTPQMARVVRGVVLSVKEHEYVEGARAMGGGALRILGRHILPNIVSPI
                     IVM"
BASE COUNT          495 a         1031 c          989 g          542 t
ORIGIN      
        1 ccttcaagct ccgccgcggc gtgaagttcc atgacggcac gccgatgaac gctcgcacgg
       61 tggccgagga cctcgaccgc gcgatcaatc ccaagaaccc gtgctacgtc ctcgaccgca
      121 aggtcgacac ctacgacacc ttcacctttg gctcggtgaa ggcgggcgac gccgtgaaga
      181 tggatgtact cgacgactac accctccgct tctcgctgcc caagccgaac gccccgttcc
      241 tctcgagcct cgccatggtg tggacgggca tcatgagccc ggcggcgacc aagcagtaca
      301 actgtgacgc gggccagcac ccggtcggca ctggcccctt caagttcgtg gaggccgtcc
      361 ggaacgatca catcaccgtg gatgcgaacc ccgagtattg gggggggaag cccaaggtgg
      421 accggattat cttccggatc atccccgaga gcgccacccg ggtgctggca ttggagcgta
      481 acgaggtcca gatcctggcc gacgtcctgc cggcggatta cgcgcgcatc gagaagaact
      541 ccgccctcaa gctttaccgg cagccgggtc tcacgatcct cggggtcgcc atgtcgaatg
      601 atctcggccc cttcaaggac aagcgggtcc ggcaggccat gaactacgcc gtcgacaagg
      661 aggcgatcaa caagggcctc tacggcggcg cgaccaccgc aagccagggg atgcccccgg
      721 tcctgtgggg gtttgccaag aacgtccaac cctacccgta cgacgtgaac aaggccaagg
      781 ccctgctcaa ggaggcagga tacccgaatg gcttctcgac cgagatgatg gtctccgcga
      841 atccccgcgg ctataacccg atcggcgggg ccaagctcgg ggaggcagtg cagggctacc
      901 tggccaaggt cggcgtgaac gtcaagatca gccagtacga gtggggggcc tacctggata
      961 gattccggca cacaccctgg gaagggttcg ccatcgccgg gtggtcgggc gacaacggcg
     1021 accccgacaa cttcctcggc gacctgttcg agtgggacac ccctgccaac aaggcccggg
     1081 tcaacaacac ggggcgacac cacaaccccg agtatgacaa gctgatggtg cagggacgcg
     1141 agacgaccga tcaggccaag cgcgtccaga tctacgagaa ggcgaaccag atcctgcacg
     1201 acgacgcggc ctggatcttc atcaaccaca ccaaccaggt ccgggcggca cgggcaaacg
     1261 tgaagggttt ccaactcaac cccctgcaga tgttcttcca catggagcag gtctcgatcg
     1321 agtagcggtc gtccgggtag tcgcgtatcg tgacgggtcc gccgcccgcg ggcggcggac
     1381 ccccgccact cctggtctga aggtcaactc accccgatca tgtggaaata cgccgcgcgg
     1441 cggctcgtcc agatgctccc cgtcctcctc ggggtcagcc tcctggtctt cctgggcatg
     1501 cacctgattc ccggggacgt ggcgcagctc ctgctgggtg acaaggggag cgcggagcag
     1561 ctcgagcgtc tccgccacca gctcggtctg gaccagccca tctacgtcca atacctccgg
     1621 ttccttgccg aagctctgcg cggggatttc ggcgactcga tccgcacgcg acaacccgtg
     1681 atctgggaga tcggccaagc gctcccggtg accatccagt taagcctggc cgctctcgcc
     1741 ttcgcggttg tggtcggcct cgtgatcggc gtggtggcgg cgcgacgccc gcactccagc
     1801 ctcgacacgg gcaccatggt cttcgtgctg atcggggtct ccatgcccgt gttctggacc
     1861 gggatcctgc tcctgctggt gttcgggggg atcctgggct ggctccccct ggggggggtg
     1921 ctggacgccg ggatgagcct tcgccgcgtg acggggatgc cggtcgtgga tgccctggtc
     1981 tccggcaact gggcggccgc gcgaagcggg ttcgcgcacc tgatcctccc cgcggtcacc
     2041 ctgggtgcga cggtcatggc caccatcgcc cggatggctc gctccagcat gctggatgtc
     2101 ctcaacctcg attacgtccg cacggcgcga gccaaggggg tggccgagcg gcgggttgtc
     2161 cgccgtcacg ccctctggaa tgccctcttg ccggtggtca cgctgatcgg gcttcaactg
     2221 gggttgttgc tctcgggcgc ggtgctgacc gagacgatct ttgggctccc cggtcttggc
     2281 agactgacga tcacgagcgt gctggcacgg gactacccgg tcgttcaggg cgtggtggtg
     2341 atcggcgccg tgatcttcgt gctggccaac ctcgtcgtcg acctccttta cgcatggctc
     2401 gatccgcgga ttcgttacag ttgagcgcgg ccggatcgcc tttcgctgcc gagggtcggg
     2461 cggcgaccct gtcctggcga cggctccggc ggagtcccaa tctcgtcgcg ggcgccgcca
     2521 tcctgttgct ggtcctcctg tccgccctgc tggcgcctca ggtgtccccc tacgacccga
     2581 tcgatcagga gttcaccagt cagttgcagc cgccgtcggc aagccacctc tttggcaccg
     2641 acgagttcgg cagagatatc ttctcccggg tcatcttcgg cgctcgcatc gccctgagcg
     2701 tgggaggcct ggccgacgcc atcgcgacgg cgctgggcgt actcctgggg gtgatggccg
     2761 gctacttcgg cggccgactg gacgccgtcg tcacccgcgt gatcgacgtg ctgttggcct
     2821 tcccgtacct cttgctggcg atgatcgtcg tggcgattct gggacccggc ctgaccaacg
     2881 ccatggtggc catcgctatc gtctacaccc cccagatggc gcgggtggtc cggggcgtcg
     2941 tcctctcggt gaaggagcat gagtacgtcg agggggcgcg agccatgggg ggcggcgcgc
     3001 tccggatcct gggccgtcac atcctcccca acatcgtctc cccgatcatc gtcatgg
//
LOCUS       DBNGZC010000554         1285 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2491063, whole genome shotgun sequence.
ACCESSION   DBNGZC010000554 DBNGZC010000000
VERSION     DBNGZC010000554.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1285)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2491063"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /gene="asnB"
                     /locus_tag="AC16G0_09660"
     CDS             complement(<1..771)
                     /gene="asnB"
                     /locus_tag="AC16G0_09660"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR5099916.1"
                     /translation="MCGIVGIVKLDPRVPVDETRLKRMRDVLYHRGPDGEGLWIDGPV
                     GFGHRRLAIVDVAGGSQPMANEDESVWIVYNGEIYNHPTLRPELEKRGHRYRTRSDTE
                     TILHLYEEEGERCVERLQGMFAFALWDRARERLLLARDRLGIKPLYYACVGDELLFAS
                     EIKAILAAGSLSPEFNTAILPEYLATRFTSGKETFFRGVLKLLPGRTLSWSLAVGFRE
                     RRYWRLPVETDGARTTLREQAGELRARLEAAVQSHLMSD"
     gene            complement(888..>1285)
                     /locus_tag="AC16G0_09665"
     CDS             complement(888..>1285)
                     /locus_tag="AC16G0_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099917.1"
                     /translation="EVTVGSGLWARACGAEGHGLLIRPFAGVPLKADVVEGGLDPIQG
                     WVSPDYGRRQPAPVLIYSTESHLPVRILTLLFPTKDPLAPPPPVSPLGEGGPGPAGLL
                     FDDEDEVVRFDDPDGVTVHLSSASGIGEA"
BASE COUNT          236 a          447 c          391 g          211 t
ORIGIN      
        1 atcgctcatc aggtggctct gaacggccgc ctccagccga gcacggagct cgccggcctg
       61 ttccctcagg gtcgtccggg ccccatccgt ttcgaccggt agccgccagt aacgccgctc
      121 ccggaacccc acggcaagcg accaggaaag cgttcttccc ggcaggagct tcagcacccc
      181 ccggaagaac gtctccttcc cggaagtgaa acgggtggcc agatattcag gcaggatggc
      241 ggtgttgaac tccggggaga gggagccggc cgccaggatg gccttgatct ccgaggcgaa
      301 tagcagttcg tcgccgacgc acgcatagta gaggggcttg attccaagcc ggtctcgcgc
      361 caggagcaac ctctcccgcg cccgatccca gagggcaaag gcgaacattc cctggaggcg
      421 ctcgacacaa cgctccccct cctcctcgta aagatgcagg atcgtttccg tgtcgctccg
      481 tgtgcggtag cggtggccgc gcttctccag ttccggtcga agcgtcggat ggttgtagat
      541 ctcaccgttg tagacgatcc agacggactc atcctcgttg gccatcggct gggagccgcc
      601 cgcgacgtcc acgatggcga gtcgccggtg cccgaatccg accggcccgt cgatccagag
      661 tccctcgccg tccggtcccc ggtgatacag cacgtcccgc atgcgcttga gccgcgtttc
      721 gtccacgggg acccgcggat cgagcttcac gataccgaca atcccgcaca tcgagatacc
      781 ctcgcttcct aattgatcac gcttgctgag ggtggagcca cagaccctct ctgactttgg
      841 ccctcattcc cctgcacatt cccagagcgc ggcggacccc caagggatca cgcctcaccg
      901 attccactcg cgctggacaa atggacggta acaccatccg ggtcgtcgaa gcgtaccacc
      961 tcatcctcgt cgtcgaacag caggccggct ggtcccggcc ccccctctcc cagcggggag
     1021 acaggaggag gaggggcaag cgggtccttt gtggggaaga gcagggtcag gatgcggacc
     1081 ggcagatgcg actcggtcga gtaaatgagg acgggggcgg gttggcggcg gccatagtcc
     1141 ggggagaccc aaccctgaat gggatcgaga cctccctcga ccacatcggc cttgagcggc
     1201 actcccgcaa acggtcggat gagcagccca tgcccctcgg ccccacaggc cctggcccag
     1261 agtcccgagc ccaccgtcac ctcca
//
LOCUS       DBNGZC010000555         1240 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_343819, whole genome shotgun sequence.
ACCESSION   DBNGZC010000555 DBNGZC010000000
VERSION     DBNGZC010000555.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1240)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_343819"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="AC16G0_09670"
     CDS             <1..273
                     /locus_tag="AC16G0_09670"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HIR5099918.1"
                     /translation="EVREIVVAGGGSQNAYLMRQLAATLPGSIVMASDAVGFPTHTIE
                     AVAFACLAYLTATGRPGNLPAVTGARGPRVLGCIVPGRGFRAVGGS"
     gene            281..1009
                     /locus_tag="AC16G0_09675"
     CDS             281..1009
                     /locus_tag="AC16G0_09675"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HIR5099919.1"
                     /translation="MTPYHEAAMRLLTAIADSQAENLERAARTVFESLSSDGVLHVFG
                     SGHSHALSEEVYHRAGGLVPVNAIQEAFLTPLTPPGMNRQMERLPGLAKIILDGHDLR
                     PGEVLMVISNSGINVVPIEVALEGKARGLTLIALTSLNHSQSVPARHPSGKRLFDIAD
                     IVLDNCGVAGDAAVQYAGLPGRVGPTSLLAGSYIINSLVCRVVELFLANGLTPPVYMS
                     ANVHGGDEHNTALEAKYRGRIRGL"
BASE COUNT          218 a          390 c          415 g          217 t
ORIGIN      
        1 gaggtgcgcg aaatcgtggt ggcagggggc ggctcccaga atgcctacct gatgcggcag
       61 ctcgcggcga cgctaccggg ctctatagtc atggcctccg acgcggtggg gtttccgacg
      121 cacaccatcg aggccgtggc cttcgcctgc ctcgcctatc tcacggcgac gggtagaccg
      181 ggcaacctgc cggcggtcac gggcgcgcgg ggacccaggg ttctggggtg catcgtgccc
      241 ggacgggggt tccgagcagt gggaggaagc taatatgggg atgactcctt accatgaggc
      301 cgccatgcgg ttattgacgg cgatcgcaga ctcccaggca gaaaacctag agcgcgccgc
      361 ccgaacggtc ttcgagtcgc tctcgtcgga cggcgtgctt cacgtcttcg gcagcggtca
      421 ctcgcacgcg ctgtccgaag aggtctatca ccgggcgggc ggcctcgttc ccgtcaacgc
      481 aatccaggag gcttttctca cacccctcac gccccccggg atgaaccgcc agatggagcg
      541 cctccccggt ctggccaaga tcatcctgga tggccacgac ctccgtcctg gcgaggtcct
      601 catggtcatc tccaactccg ggatcaacgt cgtgcctatc gaggtggcgc tggaagggaa
      661 ggcgcgcggc ctcacgctga tcgccttgac ctcgctcaat cattcgcaga gcgtgccggc
      721 gcgccatccc agcgggaagc gccttttcga catcgcggac atcgtcttgg acaactgcgg
      781 ggtggccggc gacgctgctg tgcagtatgc aggactgccc ggacgggtgg gtcccacctc
      841 gctcctggcc ggttcgtaca tcatcaattc cctggtctgt cgtgtggtcg agttgttcct
      901 ggccaatggc ctcacgccac ccgtgtacat gagcgccaac gtccatggcg gggacgagca
      961 caacacggcg ctggaggcga agtatcgggg acgtatccgg ggtttgtgaa gggggcaggc
     1021 agcgcaggaa agcgatcggg cagattcgta ccgcacccca cgcaattgcc cctcaccctc
     1081 tcacccacgg tgagacaggg taggtgaagg ggatctcgga agaagggcag ggattcctga
     1141 cttccgaagg gaggagacgt ggccgggagg gaatcaaacg aaaggctgct cgaaggcatc
     1201 cgcgtgctgg atctgagccg gatcatcgcc ggcccgttct
//
LOCUS       DBNGZC010000556         1777 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1221545, whole genome shotgun sequence.
ACCESSION   DBNGZC010000556 DBNGZC010000000
VERSION     DBNGZC010000556.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1221545"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="AC16G0_09680"
     CDS             <1..463
                     /locus_tag="AC16G0_09680"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR5099920.1"
                     /translation="LIEAACAIAVVALTVIVSLQVFNRFVLKAPLAWSEDLAMLLFQW
                     VVFLGASLGVKRMRHFGIELIVRKFPVRMRHWVELATPVVMAIVALVMIVQGWTLLSF
                     NRTRTFPTMDLTYTWAFLPIPLAGVLILIYLVQMEINRWSGKPSTGGGHER"
     gene            460..1755
                     /locus_tag="AC16G0_09685"
     CDS             460..1755
                     /locus_tag="AC16G0_09685"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR5099921.1"
                     /translation="MSEIPVLILLGGFILLLALNVPVAFSMILACIAALWWQGTIPIS
                     TVTMKLYSGIDTFPFLAIPLFILAGALMEQGGISARLVRLASNLVGHIRGGLGFVVVV
                     SEIFFSGISGSSIADASAIGSLLLPSMVRSGYTAARATAVVAAATGMGMLIPPCLNMV
                     VLGAVANISIAGLFMGGFLPGFLMALTLMVVIYVQSNRGILPAATAARPEMKEVLVSL
                     RDSLIPLMTPVIIFGGIFGGVFTATEAAAAATIYAFIVSFFIYKEIGTKDLYRILVDT
                     AVMTGVVSLLVGAATGFSWILATHQVPRMLGDFITTVGGGQYTFLLFSIAVFLFFGMI
                     LDGLPAILIFYPILMPIAYSLGVHPLHYGVLVIAVVGIAIVAPPIGLCLVILCSLAKI
                     KLTDTIGPLVPYISILVADLVIMALWPWLVLFLPTFFKL"
BASE COUNT          246 a          572 c          550 g          409 t
ORIGIN      
        1 actcatcgaa gcggcgtgcg caattgcggt ggtggcgttg accgtcatcg tcagcctgca
       61 ggtcttcaac cgcttcgtcc tgaaggcgcc cctggcctgg tcggaggatc tggccatgct
      121 gctcttccag tgggtggtct tcctgggagc ctccctgggg gtgaagcgga tgcgccactt
      181 cgggatcgag ctgatcgtcc gaaaattccc ggtgcggatg cggcactggg tcgagctggc
      241 aaccccggtc gtgatggcta tcgtggccct ggtgatgatc gtccagggct ggacgctcct
      301 cagcttcaat cggacccgga cgttccccac catggacctc acctacacct gggccttcct
      361 gccgatcccg ctggccgggg tgctcatcct catctatctg gtccagatgg agatcaaccg
      421 ctggagtggg aagccgtcca caggtggggg acacgagcga tgagcgaaat cccagtcctc
      481 atcctcctgg gggggttcat cctgctcctg gcgctgaacg tgccggttgc gttctccatg
      541 atcctggcct gcatcgccgc gctgtggtgg caggggacga tcccgatctc cacggtgacc
      601 atgaagctct actcggggat cgacacattt ccgttcctgg ccataccgct tttcattctg
      661 gccggcgccc tgatggagca gggcgggatc tcggcgcggc tggtgcggct ggcttccaac
      721 ctggtcgggc acatccgcgg cggcctggga ttcgtcgtcg tggtctcgga gatcttcttc
      781 tcgggtatct ccggttcctc catcgccgat gcctcggcca tcggctcgct gctcctgccc
      841 tccatggttc gctctggata cacggccgcc cgggccacgg ccgtcgtggc tgcggccacg
      901 gggatgggga tgctgattcc tccctgtctc aacatggtgg tcctgggggc agtcgccaac
      961 atctccatcg ccggcctgtt catgggcggg ttcctgcccg gcttcctgat ggccctgacc
     1021 ctgatggtgg tcatctacgt ccaatccaat cgagggattc tcccggcggc caccgccgcc
     1081 cggccggaga tgaaggaagt tctggtttcc cttcgcgaca gcctcatccc tttgatgacg
     1141 cccgtgatca tcttcggcgg gatcttcggc ggggtcttca cggccaccga ggcggcagcg
     1201 gcggccacga tttatgcgtt catcgtctcg ttcttcatct acaaggagat cgggaccaag
     1261 gatctgtaca ggatcctcgt ggatacggcg gtcatgaccg gtgtcgtctc tcttctcgta
     1321 ggggccgcga cgggcttctc gtggatcctg gccacgcatc aagtcccgcg tatgctgggg
     1381 gacttcatca ccacagtcgg aggcggccag tacaccttcc tgctcttcag catcgccgtg
     1441 ttcttgttct tcggcatgat cctggacggt ctgccggcca tcctgatctt ctacccgatc
     1501 ctgatgccga tcgcctactc gctcggcgtg catccgttgc actacggggt cctggtgatc
     1561 gccgtcgtgg gcatcgccat cgtggcgcct cccatcgggc tgtgcctggt gatcctctgc
     1621 agcctggcca agatcaaact cacggatacg atcggtccgc tggtcccgta catcagtatt
     1681 ctggtcgcgg atctggtgat catggccctc tggccatggc tcgtcctgtt tcttcccacg
     1741 ttcttcaagc tgtgagggtg cggggggcga cgaggcg
//
LOCUS       DBNGZC010000557         6396 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1515408, whole genome shotgun sequence.
ACCESSION   DBNGZC010000557 DBNGZC010000000
VERSION     DBNGZC010000557.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6396)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6396)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6396
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1515408"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..1057)
                     /locus_tag="AC16G0_09690"
     CDS             complement(101..1057)
                     /locus_tag="AC16G0_09690"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR5099922.1"
                     /translation="MRSRVTHGWILVVACTVLLGVLPVGAQEYPVKPIEVIVGAAPGG
                     GTDMIARAVADVAPKYVGQPLVVVNKVGASMMIAAQYVASAKPDGYTLNVAGGSETVA
                     VPHFRSVPFDPINDFEPIIRFIIERVGFYVKADSPWKTMKDFVADAKQNPEKYTYAHA
                     GVGGIHHATMMAFEKRAGMRLTPVPHKGGAENLAALAGGHVSMAVASPNEAYALVQGG
                     RVRPLANASLKRSATEPDTPTLRELGYDVYIENQKGFVFPKGTPKPIVQKLHDNLKKV
                     FDDPQFLASAEKLKLELAYLSGEDFRKSLKAMYDQIGEAVKK"
     gene            complement(1243..1689)
                     /locus_tag="AC16G0_09695"
     CDS             complement(1243..1689)
                     /locus_tag="AC16G0_09695"
                     /inference="COORDINATES: protein motif:HMM:NF015444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003680 - minor groove of
                     adenine-thymine-rich DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HIR5099923.1"
                     /translation="MEVIKCGLISEMILARFDPGERLLEGLRELIRAERIRTGLVVSG
                     IGTLSDCRLHQSVAGYPPNLMTRHQEYLELKGSYEIASIQGIIAEGDPHLHITLSEGS
                     HTVAGHLEDGCVVMTLAEVAILRGQEAPIRRVTRGPEKIKQLTAAE"
     gene            complement(1828..2184)
                     /locus_tag="AC16G0_09700"
     CDS             complement(1828..2184)
                     /locus_tag="AC16G0_09700"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HIR5099924.1"
                     /translation="MRSNPRVHRLGLGFFDRVYRLAAKIPRGKVATYGQIARLLGAPR
                     SARIVGWAMHGNPHGSKVPCHRVVQQGGSLSPNYCIDDPGRQRRLLAREGVTFLPDGR
                     IDMARHQWSAPVKGAR"
     gene            complement(2218..3189)
                     /locus_tag="AC16G0_09705"
     CDS             complement(2218..3189)
                     /locus_tag="AC16G0_09705"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HIR5099925.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRTGIGNGWEVWGVHHGYAGL
                     IADDMLALNARDVGGILQRGGTVLGSARSAEFETVAGREKAIRNLNVRGIEALVVIGG
                     NGSQAGAACLFQMGFPVVGVASTIDNDLVGSDITIGVDTALNIALEAIDRLKTTASSH
                     QRAFLVEVMGRRCGYLALMAAIAGGSEAVVLPEFEIDPEALAAELHAAYLRGKSHAIV
                     VVAEGAKYNAEALALYFRQNQDRLGFDLRVTTLGHVQRGGEPGAFDRILATRLAGGAT
                     EALAKGEHGVLVGSVKGEIRTTPLADVVGKQKPLDPRLVALAGVLAR"
     gene            complement(3338..3589)
                     /locus_tag="AC16G0_09710"
     CDS             complement(3338..3589)
                     /locus_tag="AC16G0_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099926.1"
                     /translation="MRWTPTHRITYSPNREEPEAFEVMAFDLGCDHPGPGRYGLFTQD
                     EWDAGAPPDWTMDLEGQVWYQGAAAPGEGPATIQDLWNG"
     gene            3821..4585
                     /gene="cuyB"
                     /locus_tag="AC16G0_09715"
     CDS             3821..4585
                     /gene="cuyB"
                     /locus_tag="AC16G0_09715"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009610996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteate racemase"
                     /protein_id="HIR5099927.1"
                     /translation="MRTMSTSGKIIGILGGMGPAATADFYQKIIRATPAKADQDHLRV
                     VIYSNPQIPDRTAAIRGDGPDPVPALVESAEALIRAGADFLTIPCVTAHHFFEPLQRA
                     VRIPILHLIGESVTAVMGEHPGLRRLGLLATSGTLQSRLFEAYFEPRGFSILTPDQET
                     QSAWVMEAIYAVKHGDPLEGPRRLIHEAAEHLRTRGAEAVLAGCTEVPLILQDGDLSV
                     PVIDPTWILAQAAVCRAMASSPQHLAASQRCYKDRG"
     gene            4628..5356
                     /locus_tag="AC16G0_09720"
     CDS             4628..5356
                     /locus_tag="AC16G0_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014320805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR5099928.1"
                     /translation="MIEFRGVNKWFGSLHVLKDIDLLVKAGEVVVVCGPSGSGKSTLI
                     RCINKLEPIQKGEIVVDGMSLGDSATDITKLRAEVGMVFQQFNLYPHMTALQNITLAP
                     VKVRKQSWDEAARIARDLLVKVGIPEKAEHYPAQLSGGQQQRVAIARALAMQPKIMLF
                     DEPTSAMDPEMINEVLDVMVNLAKEGMTMIVVTHEMGFAKKVAHRIIFMDEGRIVEEG
                     DPQRFFASPKEERTRTFLSKILVH"
     gene            5468..6325
                     /locus_tag="AC16G0_09725"
     CDS             5468..6325
                     /locus_tag="AC16G0_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR5099929.1"
                     /translation="MRRVFLVALTTLAMLGLAASAWAAESTLEKIGRTGVFTVGTRTA
                     SPPFGFINKQNEWVGFSIDLAKLVHKNLEKKLNKSIKFELKESTPATRIPLLSSGTVD
                     LIAGTMTDTRSRRDSVDFSLTFFVTGAQFLVKADSPIQGIQDITGKRVGTQQGSTNER
                     ALREKGVQAQLVIFPDQAAAFTALVQGRVDTFTNDGIQLWGLKYKAPNPNEWKVVGDF
                     YTYEPYGMAMRKNDSDFRAVVNNTLMEAIENGEYFKIYDKWFGPKSADPYPLADSTKS
                     FLMLQVVPK"
BASE COUNT         1160 a         2151 c         1964 g         1121 t
ORIGIN      
        1 aacccccacg aggccacggc aaccgggatc gccaggagca cggcccccac gcccagaacc
       61 cgatcaacgg tcatgcgtca gcccgccagg tgtgtcggtg ctacttcttc accgcctcgc
      121 cgatctggtc gtacatcgcc ttcagggatt tgcggaaatc ctccccgctg aggtaggcca
      181 gctccagctt cagcttctcg gcgctggcca ggaactgggg atcatcgaag actttcttga
      241 ggttgtcgtg gagtttctgc acgatcggtt tgggagtgcc cttggggaac acgaagccct
      301 tctggttctc gatgtaaacg tcatatccca gctcgcgcag ggtcggcgtg tccggttccg
      361 tcgccgaccg cttcagcgag gcattggcca gggggcgaac ccgtccgccc tgcaccaagg
      421 catacgcctc gttcggcgag gccaccgcca tgctcacgtg acctccggcc agggcggcga
      481 ggttttccgc tccccccttg tgcggaacgg gggtcaggcg catgccggcg cgcttttcaa
      541 atgccatcat ggtggcatga tggatgccgc cgacaccggc atgggcatag gtgtacttct
      601 ccggattctg cttggcgtcc gccacgaagt ccttcatggt cttccagggg ctgtcggcct
      661 tcacatagaa tccgacccgc tcgatgatga atcgaatgat gggctcgaag tcgttgatgg
      721 ggtcgaaggg gacgctccga aagtggggca cggccaccgt ctcgctgcct cctgccacgt
      781 tcagcgtata gccgtccggt ttcgcgctgg cgacgtactg cgcggcgatc atcatgctgg
      841 cgcccacctt gttgacgacg accaggggct gacccacgta cttgggggcc acgtcggcca
      901 cggccctggc aatcatatcg gttccaccgc cgggggcggc ccccacgatc acctcgattg
      961 gcttgacggg atactcctgc gccccgaccg gcagcacgcc gaggaggacc gtgcaggcca
     1021 ccaccaggat ccagccgtgc gtcaccctgc tacgcatgat tgccctcctt gcgccgggcc
     1081 agccgacgcg ggatcttccg gatgccacgg gcatgctggc gcgatcgact cgtgcgatgc
     1141 gatgacacat actcctgatc ctggagcagc gtcaacggca aaaaccgagt cgagaggagt
     1201 accgaaccgc ccggagcgct tgcaagtgat tccttcgtcc ggctactcgg ctgccgtcag
     1261 ttgcttgatc ttctccggtc cgcgtgtcac ccgccgaatc ggggcctcct gcccgcgcag
     1321 gattgccact tccgccaggg tcatgaccac gcaaccgtcc tccaggtggc cggccacggt
     1381 atggcttccc tcggagagcg tgatgtgcag gtgcggatcg ccctcagcga tgatcccctg
     1441 aatcgaggca atctcgtacg accccttgag ttccagatat tcctgatgtc gagtcatgag
     1501 gttgggtggg tagccggcaa ccgactgatg cagtcggcag tcgctcaggg tgccgatccc
     1561 cgaaacgacc agacccgtcc ggatcctttc ggctcggatc agttctcgca gaccctccag
     1621 caggcgttcg ccaggatcga accgagccag gatcatctcg gaaataagac cgcacttgat
     1681 gacttccatg cgtgcctcct ctgttgcgat ccgcggccaa gcctgcctgg gtatttcctc
     1741 cggcaggact ggccgcgacc tataccaccg gccatcaggt acatcttgac gagaacgcgg
     1801 ggtgggaatg ccgggagggc tggtaagcta ccgcgcccct ttgacaggcg cagaccattg
     1861 atgccgcgcc atgtcgatgc gaccgtcggg taggaaggtc accccttcgc gtgcgagcag
     1921 gcggcgctgg cggccgggat catcaataca gtaattcggg ctcagggagc caccctgctg
     1981 caccacccgg tggcagggaa ccttggaccc gtgaggattc ccgtgcatag cccagccgac
     2041 aatccgtgcc gaccgcggcg cgcccagcaa gcgcgcgatc tgaccgtagg tggccacctt
     2101 cccgcgcggg atcttcgccg ccaggcggta tacccgatcg aagaagccca accccagccg
     2161 gtggacgcgg gggttgctcc gcatcgggac aagtcgagcc gtcatgtctg cgcgctgtta
     2221 cctggccaag accccggcca gcgccaccag acggggatcc aggggctttt gcttcccgac
     2281 cacatcggcc agtggcgtcg tcctgatctc gcccttgacg gatccgacaa gcaccccgtg
     2341 ttctcccttc gccagggctt ccgtcgcacc gcccgccagt cgcgtggcca ggatccggtc
     2401 gaaggccccc ggctccccgc ctcgctggac atgaccaagg gtcgtgaccc ggaggtcgaa
     2461 gccgagtcgg tcctggttct gtcggaagta caatgccagg gcctcggcat tgtacttcgc
     2521 cccctcggcc acgaccacga tcgcgtgtga ctttccccgg aggtatgccg cgtggagttc
     2581 tgcagccagt gcctccgggt cgatctcgaa ctcggggagc acgaccgcct ccgaaccgcc
     2641 ggcgatcgcc gccatcaggg ccagataccc gcagcgtcgg cccatcacct cgacgaggaa
     2701 agcccgctgg tgggaggagg ctgtcgtctt caaccggtcg atggcctcta gcgcgatgtt
     2761 cagggccgtg tccaccccga tggtgatgtc ggagccgacc aggtcgttgt cgatcgtgga
     2821 cgccaccccc accaccggga agcccatctg gaagaggcag gcggctcctg cctgtgagcc
     2881 gttcccgccg atgacgacca aggcctcgat gccccgcacg ttcagattgc ggatggcctt
     2941 ctcccgcccc gccaccgtct cgaactccgc actgcgggcg ctccccagca ccgtgccgcc
     3001 acgctggagg atcccgccca cgtcccgggc gttcagcgcc agcatgtcgt cggcaatcag
     3061 gccagcgtac ccgtgatgga ccccccacac ctcccacccg ttcccgatgc ctgtccgcac
     3121 cacggcgcgg atggctgcgt tcatccccgg tgcatcccca ccactggtca gaactgcaat
     3181 ccgcttcatg ggctcgctcc ctccttcaat ccgtctccgc cagagtcccg cggtcgcgcc
     3241 acggatccat cgccggcgga gccaatcccc ggatgagaag attccgaccc tcgaagcctg
     3301 aaccgccttc gcccggccga ggcgttcaca acgcaactca cccgttccag agatcctgga
     3361 tggtcgccgg cccctcgccc ggcgcagccg ctccctggta ccagacctga ccctcgagat
     3421 ccatggtcca atccggaggg gcgccggcat cccactcgtc ttgcgtaaac agaccgtacc
     3481 gtccgggacc aggatggtcg cacccgaggt cgaaggccat gacctcgaag gcctcgggtt
     3541 cctcccggtt gggggagtag gtgatgcggt gcgtcggtgt ccagcgcatg ggctcctccc
     3601 cttctctggt aggcaggact ggagagtagc cccaaggctc gcgcggcgtc aaggcatcct
     3661 ggatcaatgt gttgaattcg gaaccggcag ggacgggggc tggccgtggc gagacctgtc
     3721 catctcaagg cggaaacgcc gacgccgacc tcatccccgg tttcgggcat tcccggcgcc
     3781 cgtccccatc cttgacacac ccacgacaga atgatacaaa atgcgtacaa tgagcacttc
     3841 ggggaagatc atcgggattc tgggggggat gggaccggct gcgaccgccg acttctacca
     3901 gaagatcatc cgggccaccc ccgccaaggc cgaccaggat catctcaggg tcgtcattta
     3961 cagcaacccg cagatcccag atcgcacagc ggccatccgg ggcgacggac ccgaccccgt
     4021 cccggccttg gtggaaagcg ccgaggcgct gatccgggcc ggcgccgatt tcctcaccat
     4081 tccttgcgtc actgcccatc acttcttcga accgctccag cgggccgtcc ggatccctat
     4141 cctccatctg atcggggaaa gcgtgacggc ggtgatgggt gagcacccgg gcctccgccg
     4201 ccttgggctc ctggcgacat cgggtaccct gcagagccgt ctgttcgaag cctacttcga
     4261 gcctcgtggc ttcagcatcc tgaccccgga ccaggagacc cagagtgcct gggtgatgga
     4321 ggcgatctac gccgtcaagc acggcgaccc cctggaggga ccgcgtcgcc tgatccacga
     4381 ggcggcggag catcttcgca cccgcggggc cgaggccgtt ctcgccggct gcaccgaggt
     4441 gcccctcatc cttcaggacg gagacctgtc cgtgcccgtc atcgatccca cctggatcct
     4501 ggcccaggca gcagtatgcc gggccatggc ctcgagccct cagcacctcg ccgccagcca
     4561 gcgatgctac aaagaccggg ggtgaggctg ggatccccac gaccctgacc cgaggagacc
     4621 tcccctcgtg atcgaattcc gtggcgtcaa caaatggttc ggatccctgc atgtcctgaa
     4681 ggacatcgat ctgctggtga aagccgggga ggtggtggtg gtctgcggtc ccagcggatc
     4741 cggcaagagc accctcatcc gctgcatcaa caagctggag ccgatccaga agggcgagat
     4801 cgtggtcgac ggcatgtcgc tcggcgattc cgccacggat atcacgaagc tgcgggccga
     4861 ggtcgggatg gtcttccagc agttcaatct ctacccgcac atgacggcgc tccagaacat
     4921 cacgcttgct cctgtcaagg tccggaagca gtcctgggat gaggcggcac gcattgcccg
     4981 ggacctgctg gtcaaggtgg gcatccccga gaaggccgaa cactacccgg cccagctttc
     5041 cggcggccag cagcagcggg tggccatcgc ccgggccctg gccatgcagc ccaagatcat
     5101 gctgttcgac gagcccacct ccgccatgga cccggagatg atcaatgagg tgctggatgt
     5161 catggtcaac ctggccaagg agggcatgac catgattgtc gtcacccacg agatgggttt
     5221 tgccaagaag gtcgcccacc ggatcatctt catggacgag gggcggatcg ttgaggaggg
     5281 ggacccgcaa cggttcttcg ccagccccaa ggaagagcgg acacgcacgt tcctgagcaa
     5341 gatcctggtg cactgaactc tcttggcagc atgtgattgc cgaggatcga ccggcggccg
     5401 tggggaagtc tcttccccat ggctggcgcg gggtggtgca tcgcactcga catgaggagg
     5461 aggggaaatg cgacgggtat tcctggttgc gctaacaaca cttgccatgc tgggcctggc
     5521 tgccagcgcc tgggcggccg agagcacgct ggagaaaatc gggcggaccg gcgtattcac
     5581 ggtggggacc cggacggcct cgcccccctt tgggttcatc aacaagcaga acgaatgggt
     5641 cggcttctcc attgatctgg ccaagctggt ccataagaat ctcgagaaga agctgaacaa
     5701 gtccatcaag ttcgagctga aggagagcac gccggcgacc cgcattccgc ttctctccag
     5761 cggaaccgtc gacctgattg ccgggaccat gaccgatacc cgctcgcgcc gcgacagcgt
     5821 ggacttcagc ctgaccttct tcgtgaccgg cgctcagttc ctggtgaagg ccgacagccc
     5881 catccagggc atccaggaca tcacgggaaa acgggtgggc acccagcaag gctcgaccaa
     5941 cgagcgggct ttgcgtgaga agggagtcca ggcacaactg gtgatcttcc cggatcaagc
     6001 ggcggccttc acggcgttgg tccaggggcg ggtggacacc ttcaccaacg atggaatcca
     6061 gctctggggg ctcaagtaca aagcccccaa cccaaacgaa tggaaggtgg taggtgactt
     6121 ctacacctac gagccgtacg ggatggccat gcggaagaac gattcggact tccgggcggt
     6181 ggtgaacaac accctgatgg aggccatcga gaacggcgag tacttcaaga tctacgacaa
     6241 gtggtttggt cccaagagtg cggaccccta tccattggcc gacagcacca agagctttct
     6301 gatgttgcag gtcgtcccca agtaacgcat acctcacggc gggaactccg ggaggaccgg
     6361 cgtgtcctcc cgggatgatc ccgatcctat ccatgc
//
LOCUS       DBNGZC010000558         4246 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2592688, whole genome shotgun sequence.
ACCESSION   DBNGZC010000558 DBNGZC010000000
VERSION     DBNGZC010000558.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4246)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4246)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4246
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2592688"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="AC16G0_09730"
     CDS             complement(<1..877)
                     /locus_tag="AC16G0_09730"
                     /inference="COORDINATES: protein motif:HMM:NF013662.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HIR5099930.1"
                     /translation="MKTLTSFEDLRALRQRLTEEATREGQRPCLVLCAGTGGQASGSN
                     TILRLVKRYILEHKLQGRISLRITGCQGFCQMDPFIVVEPGGQLYPRLRKEDIPRVIE
                     AALAGGVAEDLLYRIPGEDFPALSQGEIPFFARQQRLLLGQNRRLDPIRIHDYLGLGG
                     YAALEKVLAQADPDWVVQEITRSGLRGRGGAGFPTGRKWEMARRSHGPSGIKYLVCNA
                     DEGDPGAYMDRSLLEGNPHAILEGMIIGAFAIGATHGLIYVRTEYPLAIKHALIALRQ
                     ARELGLLGSRILGSEL"
     gene            complement(929..1780)
                     /locus_tag="AC16G0_09735"
     CDS             complement(929..1780)
                     /locus_tag="AC16G0_09735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013426.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE family
                     protein"
                     /protein_id="HIR5099931.1"
                     /translation="MTPEGVSRIVARHPSRPGRLIAILEEIQASYGFLPQEALRRVAE
                     ETGAPLVDVYGVATFYNAFSLKPRGKHLISACRGTACHVRGGVAVFRELVQQLRISPG
                     ETTPDKEITLETVNCLGACALGPVVVVDGHYFPNVTRPQVEELIRRARVGLDRVDIHT
                     DERIFPVDVACFRCHHTLMDAHYYIDGYPSIRVTASFGAMHGWIRLSCLYGSFAVDYE
                     HAFPPDAVVHFFCPHCHAELAGMSQCDRCEAPMVPMYVRGGGVVQICSRRGCKGHMLD
                     LRVAGME"
     gene            complement(2426..3778)
                     /locus_tag="AC16G0_09740"
     CDS             complement(2426..3778)
                     /locus_tag="AC16G0_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015852063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR5099932.1"
                     /translation="MTTAAGRESVLVVDDAPETLEVLRRNLESAGYRVFTVPGVAEAL
                     AALEAAPIDLVITDYKMPTVSGLDLVRHIRENLRDTEVVMITGYASIGGAIEAIKAGA
                     EEYLAKPFTEEELLDAVRRALEKRNRRRTADPDSPTNVVMPHGLIGESEAIRQVIAAI
                     RKAAETSATGLITGESGTGKELVARAIHYGSPRSSASFVPVNCGGIPEGLIESELFGH
                     MKGAFTGALTSRAGFFLTADGGTIFLDEISELGLPLQVKLLRVLQDKEVFMVGATRSR
                     LVDVRILAATNKDLRRLVERQAFREDLYFRLNVIAIEVPPLRGRGDDVLLLARHFAEK
                     YARELGRPVPRFSQGVIESFLAYPWPGNIRELENIIQRLVVMTEEEQIDVPSLPSAMR
                     FSAARPGGLHRSLAEVEGEHIRAVLASVGENKTLAAKILGIDRKTLREKLKTPTTEES
                     "
     gene            complement(3775..>4246)
                     /locus_tag="AC16G0_09745"
     CDS             complement(3775..>4246)
                     /locus_tag="AC16G0_09745"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012805529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5099933.1"
                     /translation="VSLTRIVEEGIPFLEHRVRKARIEIRRKVDPDLPEILADPNQLY
                     QVLVNLVVNAIQAMPEGGWLSIETYAADGCAVLAVEDQGVGMTPQALRRIFEPFFTTK
                     DAGEGTGLGLWVVQGIVASHGGTVRVQSEPGKGSRFEVRLPIPPLEPPSSSPSE"
BASE COUNT          728 a         1394 c         1375 g          749 t
ORIGIN      
        1 cgagctcgct gccgagaatg cggctgccga ggagccccaa ctctctggcc tggcgaagcg
       61 cgatgagggc gtgcttgatg gccagggggt actcggtgcg gacatagatg aggccgtggg
      121 tcgcgccgat ggcgaaggcg ccgatgatca tcccttcgag gatggcgtgg gggtttccct
      181 ccaggaggct ccgatccatg taggcgccgg gatcgccctc atccgcgttg cacacgaggt
      241 acttgatccc ggaggggccg tgggaacggc gagccatctc ccatttcctg ccggtgggga
      301 acccggcccc gccccgccct cggaggccgg accgcgtgat ctcctggacg acccagtccg
      361 gatcggcctg cgccaggact ttttccaggg cggcgtatcc gccgagccca aggtagtcgt
      421 ggatacgaat gggatcgagt cgccggttct gccccaggag gagccgctgc tgccttgcga
      481 agaacgggat ctcgccctgg gacagggcag gaaaatcctc cccgggaatc cgatacagca
      541 agtcctcggc gacgcccccg gcgagggctg cctcgatcac gcggggaatg tcctctttcc
      601 ggagcctcgg gtagagctgg ccgcccggct ccacgacgat gaagggatcc atctgacaga
      661 acccctggca gccggtgata cgcagggaga tcctcccctg cagcttgtgc tccaggatgt
      721 atcgcttcac gagccgcagg atggtgttgg acccgctggc ctggccgccc gtcccggcgc
      781 agagcacgag gcacggtctt tgcccctccc gtgtagcctc ctccgtgagg cgctggcgga
      841 gcgcgcgtag gtcctcgaag gatgtaagcg tcttcatcgg ctcctctgtc cccggatcgg
      901 tcgggcacct ctcgccgtcg cgctcggact actccatacc cgccacgcga aggtccagca
      961 tgtggccctt gcatccccgt cgcgagcaga tctgcaccac cccgccaccc cggacgtaca
     1021 tgggcaccat gggggcctcg cagcggtcac attgcgacat cccggccagc tcggcatggc
     1081 agtggggaca gaagaagtgg acgaccgcgt ccggggggaa ggcatgttcg taatcgacgg
     1141 cgaagctgcc gtagaggcac gacagccgga tccagccgtg catggcgccg aacgaggccg
     1201 tgacgcggat ggagggatac ccgtcgatgt agtagtgggc atccatcaac gtgtgatggc
     1261 agcggaagca ggccacgtcc acgggaaaaa tcctctcgtc ggtgtggatg tccacacggt
     1321 ccagaccgac tctggcccga cggatcaact cctcgacctg cggccgcgtg acgttgggga
     1381 agtaatggcc gtccacgacc accacgggcc ccagcgcgca cgcccccagg cagttcacgg
     1441 tttcgagggt gatctccttg tcgggggtcg tctcccccgg actgattcgg agctgctgga
     1501 cgagctcgcg aaagacagcc accccgcctc ggacatgaca ggccgtgcca cggcacgccg
     1561 agatgaggtg cttgcccctc ggtttcagac tgaatgcatt gtaaaaggtg gcgacgccgt
     1621 agacatcgac gaggggggcc ccggtctcct ctgcgaccct gcgtaaggcc tcctggggga
     1681 gaaagccgta actggcctgg atctcttcca ggatggcaat cagccgccct ggtcggctcg
     1741 gatggcgcgc aacgattctc gatactccct ctggtgtcat aggtacccct cgcgcggaat
     1801 tccgccgtag agtgcctggg gcatcacgcc agctcctcgc agtgccagac cccgcccgga
     1861 ggtttcgggt accggcagtc gagccggcga gcgcactctc gacacaggcc attgagctcc
     1921 ttggcctcgg ctgtgtccgc catgctcggc ggggcagagg tctggccacc gggcaccgcc
     1981 gggcgagacg gagtgaactc gcccccctcg aattcctcgc aagaccagac cggacgggac
     2041 ggatcgcgcg ggaaggtgca ggtgtcagcc gccacacaat cccggcacag cccctcgttt
     2101 ttttgtatct cacgcgcggc tgggcgcttc accacccttg gcatcgcctc tagcttgaac
     2161 atcatcccaa ccccctttcc cgggcggtca tccaaagctc gaaagtgccg tccatctcac
     2221 atagcatcct cttgagcaac gacggtgcca ggagatgtcg ccggggacgg ccagacacgg
     2281 cctcggaaaa gcaagcgcga acaccttgat ttcaaggggc ttcccgccag acggctgggt
     2341 tccagtattg attgcgatca ggaagggcag gacgatgcgg gatggggaga tcggccccgc
     2401 tggggaaatt ttctccggtg ggatttcagg attcttccgt ggtcggggtc ttcagcttct
     2461 cgcgcagggt cttgcggtcg atgcccagga tctttgccgc tagcgtctta ttttcaccga
     2521 cgctggccag cacggcacga atgtgctcgc cctccacctc ggcaagggag cggtgaaggc
     2581 cgccgggtct ggccgccgag aaccgcatcg cggacgggag actgggaaca tcgatctgct
     2641 cctcctcggt catgacaacc agacgttgga tgatattttc cagctcacgg atgtttccgg
     2701 gccagggata ggcgaggaag ctctcgatga ccccttggga gaatcggggt acaggccgtc
     2761 ccagttcgcg ggcgtacttc tcggcgaagt gtcgggccag gaggagcacg tcatcccctc
     2821 gcccgcgaag cggggggacc tcgatggcga tcacgttcag gcggaagtat agatcttccc
     2881 ggaacgcctg tcgctcaacc aagcgccgga gatccttgtt cgtcgcggcc agtatccgta
     2941 cgtccacaag ccgggagcgc gtggcgccta ccatgaagac ttccttgtcc tgcagcacgc
     3001 gcaggagctt cacctggagc ggcaggccca actcgctgat ctcatccagg aagattgttc
     3061 cgccgtcggc ggtcaggaag aagccagccc gggaggtcag ggcccctgtg aatgcaccct
     3121 tcatatgtcc gaagagctcg ctctcgatca gaccctcggg gattccaccg cagttcaccg
     3181 ggacgaacga ggcggacgac cgggggcttc cgtaatggat cgcccgcgcc accaactctt
     3241 tgccggtccc gctttcgccc gtgatgagac ccgtggccga ggtctcagca gccttccgga
     3301 tggcggcgat gacctgccgg atggcctcgg actcgccgat gaggccgtgc ggcatcacga
     3361 cattcgtcgg ggaatcggga tcggcggtcc tccggcggtt tcgcttttca agggcccgtc
     3421 gcacggcgtc aagcagctcc tcctccgtga agggcttcgc caggtactct tcggcgccgg
     3481 ccttgatcgc ctcgatggcc cctccgatgg aggcgtagcc ggtgatcatg acgacctcgg
     3541 tgtccctgag gttctctcgg atgtggcgca ccaagtccag gccgctcacc gtcggcatct
     3601 tgtaatcggt gatgacgagg tcgatggggg cggcctccag cgctgccagg gcctcggcca
     3661 ctccggggac ggtgaagaca cggtaccccg ccgactccag gttgcgccga agcacctcca
     3721 gggtctcggg ggcgtcgtct acgacgagaa cggattctct gcccgcagcc gtcgtcattc
     3781 ggatggactc gaggatggag gctccagggg cgggatcggc aggcggacct cgaaccggct
     3841 tcctttcccg ggctcgctct gtacccggac ggtgccgcca tgcgacgcca cgatcccctg
     3901 aaccacccag aggcccaggc cggtgccctc tcccgcgtcc ttggtcgtga agaacggctc
     3961 gaagattcgc cgcaatgcct ggggcgtcat gccaacgcct tggtcctcga cggccaggac
     4021 ggcgcaacca tccgcggcgt acgtctcgat ggacagccag cccccctcgg gcattgcctg
     4081 gatggcgttc accaccagat tcaccagaac ttggtagagc tgattcggat cggcgaggat
     4141 ctcgggcagg tcggggtcaa ccttccggcg aatctcgatc ctcgccttgc ggacgcggtg
     4201 ctccaggaag gggatcccct cctccacgat tcgcgtcagg ctgacc
//
LOCUS       DBNGZC010000559         1339 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1222168, whole genome shotgun sequence.
ACCESSION   DBNGZC010000559 DBNGZC010000000
VERSION     DBNGZC010000559.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1222168"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="AC16G0_09750"
     CDS             <1..342
                     /locus_tag="AC16G0_09750"
                     /inference="COORDINATES: protein motif:HMM:NF042714.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pYEATS domain-containing protein"
                     /protein_id="HIR5099934.1"
                     /translation="QKGRWGGQREADYRVVVAGPIRPLRSDPSNYRIPLEVRSTDPAG
                     HPLAGAVRFHLHNTFSPDVQEVAVRDCVARLGLVAYGAFTVGVETDDGARLEINLADA
                     DIDAPQVFKDR"
     gene            complement(329..631)
                     /locus_tag="AC16G0_09755"
     CDS             complement(329..631)
                     /locus_tag="AC16G0_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099935.1"
                     /translation="MGITTYRGEPSIEAIADRVYAELRPASRKKAVAALLRENPHLRQ
                     LDRVETGAILTVPELPGLRIAQNPGTPTASRPHAADAAPGERRRAHRAQRLRASDP"
     gene            complement(748..1272)
                     /locus_tag="AC16G0_09760"
     CDS             complement(748..1272)
                     /locus_tag="AC16G0_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018078747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098015 - virus tail [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HIR5099936.1"
                     /translation="MAQFSVNAQRLDPYKNFKFRVKWDGRYVAGVSKVSALKRSTELV
                     EHRQGGDPSTSHKSPGRTKYEAITLERGVTHDTEFEKWANKVWNYGSGLGAEVSLKDF
                     RKDLIIEVYNEAGQLALAFKVYRCWVSEYQAIPDLDANANAVAIQHIKLENEGWERDY
                     DVTEPSEPSFTEPA"
BASE COUNT          185 a          454 c          442 g          258 t
ORIGIN      
        1 cagaaggggc gctggggcgg ccaacgcgag gcggattacc gggtcgtcgt cgccggcccc
       61 atccggcccc ttcgctcgga tccctcgaat taccggatcc cgctcgaggt ccggtccact
      121 gacccggcgg ggcatcccct ggccggcgcg gtgcgcttcc atctgcacaa cacgttctcc
      181 cccgacgtgc aggaggtcgc ggtccgggac tgtgtggcgc gcctcgggct cgtcgcctac
      241 ggcgcgttca cggttggggt ggagacggac gacggtgccc gtctcgaaat caatctggcc
      301 gacgccgaca ttgacgcgcc tcaggtcttc aaggatcgct agcgcggaga cgttgcgcgc
      361 gatgggctcg ccggcgctcg cccggggcgg cgtccgctgc gtgcggacgt gatgctgttg
      421 gcgtgccagg attctgagcg atgcggagtc ctggaagctc cggaacggtc aggatggcgc
      481 cggtttcgac cctgtccagc tggcggaggt gcgggttctc tctcagcaag gccgccacag
      541 ccttcttgcg ggaggctgga cgcagttccg cgtacacccg gtcggcgatg gcctcgatcg
      601 agggctctcc ccgatacgtg gtgataccca tggccctacg ctccttctgt cagactccga
      661 cgaggttcag ggctgcagcc agtgctgtta gcgcctccgc gttgacagcg cccttgctca
      721 cgcggccgac ggcggctgca tcgggcgcta cgccggctcg gtgaagctcg gctccgaggg
      781 ctcggtgaca tcgtagtccc gctcccagcc ctcgttctca agcttgatgt gctggatcgc
      841 caccgcattc gcattggcgt ccagatccgg gatcgcctgg tactccgaga cccagcagcg
      901 gtagaccttg aaagccagcg ccagctgccc ggcctcgttg tagacctcga tgatcaggtc
      961 cttccggaag tccttcagcg acacctcggc gcccaggccg gagccgtagt tccagacctt
     1021 gttggcccac ttctcgaatt cggtgtcgtg ggtgacaccc cgctccaggg taatggcctc
     1081 gtacttcgtc cgccccggcg atttgtgcga ggtggagggg tcgccaccct ggcggtgctc
     1141 gaccagctcc gtgctgcgct tgagcgcgct gaccttgctg acccccgcca cgtagcgtcc
     1201 gtcccacttc acccggaact tgaagttctt gtagggatcg aggcgctggg cgttgacgct
     1261 gaactgtgcc atggctggtc tcctaggccg cgacctggcc ggccatctgc tggatcttga
     1321 tgaccacgaa ctccgccgg
//
LOCUS       DBNGZC010000560         5519 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1710617, whole genome shotgun sequence.
ACCESSION   DBNGZC010000560 DBNGZC010000000
VERSION     DBNGZC010000560.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1710617"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="AC16G0_09765"
     CDS             <1..281
                     /locus_tag="AC16G0_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099937.1"
                     /translation="SLREARNREQEIRLVAEFDRAVLTREGTTRLDLPVLRAGFRNLW
                     KEGQYERIVAVGLILSKDLMEKDETLLMYHTCAVRRLAEGPSQDQVSG"
     gene            278..967
                     /locus_tag="AC16G0_09770"
     CDS             278..967
                     /locus_tag="AC16G0_09770"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099938.1"
                     /translation="MIRLADVSKTYDGAGTRVRALAGISLQIARGEFVALMGPSGCGK
                     STLLHLIAGVDLPSAGEIWVDGQALARMSDRALSQFRRDRVGLVYQFYNLLPTMSAWE
                     NVGLPALLTGAPARAARERAEKHLQEVGMLHRKDHWPHELSGGELQRVAIARALVNGP
                     AVLLADEPTGNLDSKAGREILELLGSLNTSQRVTILLATHSQEAATFAGRVIHLRDGT
                     IAPEQWPDLRE"
     gene            1114..3693
                     /locus_tag="AC16G0_09775"
     CDS             1114..3693
                     /locus_tag="AC16G0_09775"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR5099939.1"
                     /translation="MHVLIHLIRFVAWRTLTRDRMRSLVTILGVALGVAVALAIRIAN
                     DSVLDSFRNSLDHVAGKSRLQVSADEGGFDETLFPWIAATPGVARAVPVVQTVMPVAR
                     RTPAAGQTGEVLLVLGVDVLADSSIREYRGPTLDLENPLQLLTDPNAILLTERFARTH
                     GLHPGGTIRLLTPTGPKEFVIRGLLADQGAAQAMDGQLAAMDIAAAQLSFGKLGKLDR
                     VDLLLDAGADPDQVAAFLRQRLPPEVTVERPEARNAQVEQMLASFQLNLFVLSLIALF
                     VGMFLVYNSLSVSVVRQRREISILRALGASRANVLAAVGAEGALIGLIGSLLGVILGA
                     ALARETVQAVSQTVSSLYAFVRPGEVHLPATLITLAVLLGSGTSILSSLLPALEAAAV
                     TPREGVAAAALERRHRPWLLASAGLGLVLLSYGLAQAGPVGRRPLFGYGAALSLLLGA
                     TLLCPASLRVFQRLLAASLAGSRLLGGRLAAGNLGRGLRRNAVTIGAVVVGLAMLVSV
                     STMVQSFRRTVEVWIQQTIRGDLYLSRATRLIKGADTRLPLSIMDEVKRIPGVAEADG
                     FRGIRVEDGQGGRFLLGAGDFAVMARRGRLLFRRGDTAAILSGARTQDQLIVSETFAE
                     RYHLREGDEVSLHAPGRTIRLPIAGLYYDYTTEGGLAVMDRTLFERLWRDAWLNSIII
                     YLSPGTDPQSVRQEILRRLGRREDLVLFTNRDLKVRILEIFDQTFAITYALEIIALVV
                     AALSVLNTLLVSVLERTREIGILRSVGFTRGGVMRTILCEATFMGVLANLLGAVTGLG
                     LSLILIYVINKQSFGWTIQFTFPAVLIGEYGLLTLAASLVAGVFPAWRASRLPIAEAV
                     RYE"
     gene            3748..4863
                     /locus_tag="AC16G0_09780"
     CDS             3748..4863
                     /locus_tag="AC16G0_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HIR5099940.1"
                     /translation="MRLRAPQVTLIALALLASWQPSEDPAASAAQFRQALPGYAFSFP
                     RDHFSHDDYRTEWWYYTGHLRAQGGDEYGYQVTFFRSGVAEAQANPSRWAAKNLYMAH
                     FAISDIPRKSFHYFERVNRGGLGQAGASGREFRVWIGDWQVSGDGTTQRLQAKEGEAA
                     VDLTFASQKAPVIHGENGVSQKGAGRGQASHYYSMTRLNTEGTLTVRGKAMPVTGLSW
                     MDHEFGSTQLSSDQVGWDWFSLQLDDGTEVMLYIIRKADGRPDPFSAGTWVGVDGRTV
                     HLRQRDFSVEVLDRWQSPRDKALYPMKWRLNVPTLGLDVTVAPAFPDQELDTAKSTNV
                     IYWEGAVSALGSVQGRPVKGQGYIEMTGYAGAFRKKL"
     gene            4896..>5519
                     /locus_tag="AC16G0_09785"
     CDS             4896..>5519
                     /locus_tag="AC16G0_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012246.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HIR5099941.1"
                     /translation="MVTRAGEVRFSAVYRVSGTKRESHIRAWDICLEQTVELPFDVVP
                     EGFVRDQVVGRIESLRKCPGGYRTTISYAAGSAAGELTQLLNVLFGNISIKPGIRLEQ
                     IILPPAMLAGFRGPNFGRTGLRRQLGVPRRPLLCAALKPMGLPSEGLAELAHEFARGG
                     IDIVKDDHGLSDQPYARFEERVRLCAKAVSKANRETGFRCAYAPNITA"
BASE COUNT          931 a         1723 c         1855 g         1010 t
ORIGIN      
        1 tgtctctgcg agaggctagg aatcgcgagc aggagatccg cctcgtggcg gagttcgatc
       61 gggccgtctt gacccgggag ggtaccacac gcctcgacct cccggtgctt cgtgccggct
      121 tccgaaacct gtggaaagag gggcagtacg agaggattgt tgccgtcggg ctcatcctct
      181 caaaggacct gatggagaag gacgagacgc tgctcatgta ccacacgtgt gccgtgaggc
      241 ggctggctga gggcccatcg caagatcagg tgtcggggtg atccgcctgg ctgatgtctc
      301 caagacctac gacggggcgg ggacgcgggt gagggcgctc gccggcatct cgctccagat
      361 cgcccgcggc gagttcgtag ccttgatggg gcccagcggg tgcgggaaga gcacgctgct
      421 tcatctgatc gccggcgtgg acctgccgag cgccggggaa atctgggtgg acggccaggc
      481 gcttgccagg atgtccgatc gcgccctcag ccaattccgt cgcgaccggg tgggattggt
      541 gtaccagttc tacaacctgc tgcccacgat gtcggcctgg gaaaacgtgg ggttgcccgc
      601 cctcctcaca ggcgcacccg cacgggcggc ccgtgagcga gcggaaaagc acctgcaaga
      661 ggtcgggatg ctccatcgaa aggatcactg gccccacgaa ttgtccggcg gggaattgca
      721 gcgggtggcc atcgcgcggg cgctggtgaa cggtccggcc gtcctcttgg cggacgagcc
      781 cacggggaac ctggactcca aggcgggacg ggagatcctg gaactcctgg ggagcctcaa
      841 cacttcgcaa cgggtcacca tcctcttggc tacccacagc caggaggccg ccacttttgc
      901 cgggcgggta atccacctgc gggacgggac gatcgctccc gagcaatggc ccgacttacg
      961 ggaatgacca aatcccaatg cccaatgacc aattgcggat tgcctgtcca tgatgctcca
     1021 tgacggcgcg ccgtgtgcac ccaccaccat tggtcatttg atccagaaga cttacgagcg
     1081 ggttcgtgaa taatccgggt gaggaattcc tgcatgcatg ttcttatcca tctgatccgg
     1141 ttcgtcgcct ggcggacgct gacgcgtgac cggatgcgga gtctcgtcac catcctgggc
     1201 gtggcgctgg gcgtggccgt tgccctggcc atccggatcg ccaatgacag cgtcctggac
     1261 tctttccgca acagcctgga ccacgtggcc ggcaagagcc gactccaggt gagcgcggac
     1321 gagggcggct ttgacgagac tctgtttccc tggatcgcgg cgacgccggg cgtggcacgg
     1381 gcggtgcccg tggttcagac cgtgatgccg gtcgcaaggc ggacgccggc cgcggggcaa
     1441 accggcgagg ttctcctggt cctgggggta gatgtcctgg ccgattcgag catccgggaa
     1501 taccgggggc cgacgttgga cctcgaaaat ccgttacaac tcttgaccga ccccaacgcc
     1561 atcctgctga cagagaggtt cgcgcgaact cacggcctgc atccgggcgg aacaatccgg
     1621 ctcctcacac cgaccgggcc gaaggaattc gtcatccgcg gactcctcgc agaccagggc
     1681 gcggcccagg ctatggacgg acagttggcc gcgatggaca tcgcggcggc gcaactttcc
     1741 ttcggcaagc tggggaagct cgatcgggtg gacctcctcc tggacgcagg ggcggacccg
     1801 gaccaggtcg ccgctttctt gcggcaacgc ctgccccccg aggtcacggt cgagcgaccc
     1861 gaggcgagga acgcccaggt ggagcagatg ctggcgtcgt ttcagctcaa cctcttcgtc
     1921 ctgagtctca tcgctctctt cgtcgggatg ttcctcgtgt acaacagcct gtccgtttcc
     1981 gtggtgcgac agcgacggga gatcagtatc ctgcgcgccc tgggggcaag ccgcgccaac
     2041 gttctggcgg ccgtaggcgc cgagggggca ctgatcggcc tgatcggttc cctgctggga
     2101 gtcatcctgg gcgccgcgct ggcgcgagag acggtccagg ccgtgagtca gaccgtctca
     2161 tccctctatg ctttcgtgcg cccgggcgag gtgcaccttc ccgccacgct gatcacgctg
     2221 gcggttctcc tgggcagcgg gacgtccatc ctttcctctt tactccccgc attggaggct
     2281 gctgcggtga caccccgaga aggcgtggct gccgccgctc tggagcggag gcaccggccg
     2341 tggctcctcg cctctgccgg cctcgggttg gtgctcttgt cctatggcct cgcacaggcg
     2401 ggaccggtcg ggaggcggcc cctcttcggc tatggcgcag ccctgagcct cctactcggg
     2461 gcgacgctcc tgtgcccggc cagcctgcgc gtgttccaac gtctcttggc cgcttccctg
     2521 gccgggtcaa ggctcttggg gggacggctg gcggccggaa atctcggtcg ggggttgcgc
     2581 cggaacgccg tgacgatcgg tgccgtggtg gtgggtcttg ccatgcttgt gagcgtctcc
     2641 accatggttc agagtttccg caggacggtc gaggtgtgga tccagcagac gatccgggga
     2701 gacctctacc tgtcccgggc cacccggctg atcaagggcg ccgacacacg gctccccttg
     2761 agcataatgg acgaggtgaa gcgaataccg ggcgtggcag aggcggacgg gttccgtggc
     2821 atcagggtgg aggacgggca gggtggacgg ttcctcctgg gtgcggggga tttcgctgtc
     2881 atggccagac gagggcggct cttgttccgc cggggggaca ccgcggctat cctgtcggga
     2941 gcccgcacgc aggatcaatt gatcgtgtcc gagaccttcg ccgagcgata ccatctcagg
     3001 gagggggatg aggtcagcct gcacgctccg ggccgtacca ttcgtctacc catcgccggc
     3061 ttgtactacg actacacgac ggaaggcggc ttggccgtca tggatcgcac cctcttcgag
     3121 cgactctggc gcgacgcgtg gttgaactcg atcatcatct acctctcccc aggaaccgat
     3181 ccccagtcgg tgcggcagga aatccttcgg cggctgggca ggcgagaaga cctcgtcctc
     3241 ttcacgaacc gggacctcaa ggtccggatc ctggaaatct tcgatcagac ctttgccatc
     3301 acctacgcac tcgagatcat cgccctggtg gtagccgctc tgagcgtcct caacacgctc
     3361 ctggtatcgg tcctggagcg gacgcgggag atcggaatcc tgcgctccgt cggattcacc
     3421 cggggcggcg tcatgcgcac gatcctgtgc gaggcgacgt tcatgggcgt gctggccaat
     3481 ctcctggggg ccgtcaccgg tcttggcctt tcgctcattc tgatttacgt catcaacaag
     3541 caatccttcg gctggacgat tcagttcaca ttccccgcgg tcctgatcgg agagtacggg
     3601 ctcttgacgc tggccgcgag cctcgtggcc ggggtcttcc cggcctggcg ggcgtcgcgc
     3661 cttcccatcg ccgaggccgt gcgctatgaa tgagaccctt cggcggcttg gccgctcagc
     3721 cggcgacgcg ccacgcggaa ccgggatatg aggctgcgcg ctcctcaggt gaccctgatc
     3781 gccttggcgc ttttggcgtc ctggcagccc tcggaggatc ccgctgcctc ggcggcgcaa
     3841 ttccggcagg ccctccctgg ctacgccttc tccttccccc gagatcactt ctcccacgac
     3901 gactatcgca ccgagtggtg gtattacacc gggcacctgc gggcgcaagg tggtgatgag
     3961 tacggctacc aggtgacgtt tttccggagc ggcgtggccg aggcgcaggc caacccgtcc
     4021 cggtgggcgg cgaagaacct gtacatggcc cacttcgcca tctcggacat cccgcggaag
     4081 tccttccact acttcgagcg cgtgaatcgg ggcggcctgg ggcaggcggg tgcaagcggg
     4141 agagagttcc gtgtctggat cggggactgg caggtgagcg gggatggaac gacgcagcgt
     4201 ctccaggcca aggagggtga ggccgccgtg gacctgacgt tcgcgtcgca gaaggctccc
     4261 gtcatccatg gcgaaaacgg cgtgagccag aaaggggcgg ggcgggggca ggcctcccac
     4321 tactactcga tgactcgttt gaacacggaa ggcacgctga ccgtacgggg aaaagcgatg
     4381 ccggtgacgg ggttgagctg gatggatcac gaatttgggt ccactcaact gagttcagac
     4441 caggtcggct gggattggtt cagccttcag cttgacgatg ggacggaggt gatgctctac
     4501 atcattcgga aggccgacgg gcgcccagat cccttctcgg caggaacgtg ggtgggggtt
     4561 gacggtcgca cggttcatct ccgccaacgt gatttcagcg tggaggttct ggaccgctgg
     4621 cagagtcccc gagacaaggc tctctacccc atgaaatggc gtcttaatgt gccaacgctc
     4681 ggcctggacg tgaccgtggc gccggcgttt cccgaccagg agctagatac ggccaagagc
     4741 acaaatgtga tttactggga gggggcggtc tctgccctgg gctcggtcca gggccgcccc
     4801 gtcaaagggc agggctacat tgaaatgacc ggttacgccg gggcgtttcg caagaagctg
     4861 tgaatcgtaa caggctccaa aaggaatcaa ggggaatggt cacacgggca ggcgaggtgc
     4921 gcttctctgc ggtctatcgc gtctccggca ccaagagaga atcccatatc cgtgcgtggg
     4981 acatctgcct ggagcagacg gtcgaactcc ccttcgacgt ggtcccggag ggcttcgtcc
     5041 gcgaccaggt ggtcggaagg atcgagtcac tccggaagtg tccgggcggt taccggacca
     5101 caatcagcta cgccgccgga tccgctgccg gggaactgac ccagctcctg aatgtcctgt
     5161 tcggcaacat cagcatcaag ccgggcatcc gcctggagca gatcatcctt cctcccgcga
     5221 tgctcgcagg cttcagaggg ccaaactttg gaaggaccgg gctgcggcgg caactcggcg
     5281 tgcctcggcg gccgcttctg tgcgcggcct tgaagcccat ggggctgccc tccgagggct
     5341 tggcggaact tgcccatgaa ttcgcgcggg gcggcattga catcgtgaag gacgaccacg
     5401 gcctatccga ccagccgtac gcccggttcg aagaaagggt aaggctgtgt gccaaggctg
     5461 tttcgaaggc gaaccgggaa acgggattca gatgcgcata cgcgccgaac atcaccgcc
//
LOCUS       DBNGZC010000561         1010 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1024713, whole genome shotgun sequence.
ACCESSION   DBNGZC010000561 DBNGZC010000000
VERSION     DBNGZC010000561.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1024713"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(476..>1010)
                     /locus_tag="AC16G0_09790"
     CDS             complement(476..>1010)
                     /locus_tag="AC16G0_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099942.1"
                     /translation="KRMTVEELFRDDKNRRNGWGLRDVLYRNHDPVRQHVVIRWVEGL
                     PEKRDEPWFLMTDLARDPRSLSCLYAKRMTVEELFRDDKDRRNGWGLRDVQVTEAGRF
                     DRLLLILALAYLLLVGLGLRARRSYRVGMWSSSNDGDRCSVFTIGRAMLDRMRQHCDT
                     VFAAVAEATVMSAPNWG"
BASE COUNT          173 a          395 c          276 g          166 t
ORIGIN      
        1 tggccttgcc gccgacggtt caacaggtcg catccaccgc cccgagggag gatgctacgt
       61 ctggtgccac gccgcctgat tgagtaaagc ttcggtaaac ccgtccggct ctctggcttt
      121 cccgcccacc tgcgattaca accggcggca tgcctgttga gcctccgccg acctcctggc
      181 ctgacgcctc tccggtctcc ctcctggctg ggcacgacga caccccgacc tgttcggctc
      241 cccccacccc cctgggacca tgcccggtct gcccacgttt ggaaacggag ttcgagccct
      301 ggcgccgggc cgcctaccgg caggccatgc accagcgggc cgtccagcgc gagaagcttc
      361 tccacgagca gaacgagcaa ctccaggccc gcatccggtc cctcgaacgg cagctcttcg
      421 gacgcaaggc cgattctccg gcccccgccg acggcccagc ccctgtgagg ccgagtcatc
      481 cccagttggg agcactcatg acggtggcct cggcgacggc ggcgaacacc gtatcacagt
      541 gctgtctcat ccgatctaac atcgcacggc cgatggtgaa gacgctgcac cgatccccat
      601 cgttgctgct gctccacatc cccacgcgat agctccgccg tgcccgcagg cccaggccga
      661 ccagcagcag gtaggccaac gccaggatca gcagcagccg atcgaaccga cccgcttcgg
      721 tcacctgcac atcgcgcagc ccccagccat tgcgccggtc cttgtcgtca cggaacagtt
      781 cctccaccgt catccgcttc gcgtagaggc acgacagact ccggggatca cgcgccagat
      841 cggtcatcaa gaaccaaggc tcatcgcgct tctcgggcag gccctcgacc cagcggatca
      901 ccacgtgctg ccgcaccgga tcgtggttgc gatacagcac atcgcgcagc ccccagccat
      961 tgcgccggtt cttgtcgtca cggaacagtt cctccaccgt catccgcttc
//
LOCUS       DBNGZC010000562         1847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2104174, whole genome shotgun sequence.
ACCESSION   DBNGZC010000562 DBNGZC010000000
VERSION     DBNGZC010000562.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2104174"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..378
                     /locus_tag="AC16G0_09795"
     CDS             124..378
                     /locus_tag="AC16G0_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099943.1"
                     /translation="MRILLVEDDRKAARVLKKGLGEKQCKTCHAQAWGPADRQQHAGL
                     LAAEQSLKANPDDPKAALAVGPNNFCVYCHRRDTKWQGED"
     gene            631..1470
                     /locus_tag="AC16G0_09800"
     CDS             631..1470
                     /locus_tag="AC16G0_09800"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5099944.1"
                     /translation="MTLLVEGLSIYLDLPWSVDLAFDVLMFGSIVVGAYLFSRYVFGI
                     VGRKEEELRNSREQLRALARHLQSVREEERSRIAREIHDTLGQPLAGLKLDLAWLAGR
                     LPPDQAPLLEKLRTTLALADSTIRSVRQITTDLRPSLLDDLGLVAAIEWLAQDFQTRT
                     GIGCEFASDQPDLLLPSELSTAVFRICQESLTNVARHAHATRVRLALKAEAGHLLLTV
                     ADNGRGITEQEIASQTSVGLLGIREWALLLGGEANFMGQPGEGTTVIVRIPLKAPRGG
                     GNY"
BASE COUNT          333 a          590 c          588 g          336 t
ORIGIN      
        1 gtcggcgccc accaccagct ccaccacctg gccatggcca ggggcgagga gatggcgggt
       61 catggccact gagccgagcc gacgtggggc tcgccctgac cacgcttttc cgcccaacct
      121 gccatgagga ttctgctcgt cgaggatgac cggaaggccg ctcgggtctt gaagaagggc
      181 ctaggggaga agcagtgcaa gacctgccac gcgcaagcgt ggggaccggc cgaccgtcag
      241 caacatgccg gtctcctcgc ggcggagcag agcctcaagg ccaatccgga tgacccgaag
      301 gctgcgctgg cagtcggccc caacaatttc tgcgtgtact gccaccggag ggacaccaag
      361 tggcagggcg aggattgacg ggcaggatag caggtgggtc gtgtcgggat actctgctgc
      421 cggccacgac gtccgccgag gattcgcccc tttcgggcgt ggcgcggcct gagggcgcct
      481 cgtgacgcgg gtcgcaaccc aaagaaacct gttcttccga tcataagaga cacccagatg
      541 gttccgccgt cctcaaagac ccccagggaa cactctctcg acaagctaag actgcttgga
      601 ttctggctgc ctgtcaccgc catcggctgc atgaccttgc tggtcgaggg cctctccatc
      661 tacctcgatc tcccgtggtc ggtcgatcta gcattcgacg tcctcatgtt tggttcgata
      721 gtcgtcgggg cgtacctctt ttcccgatat gtttttggca tcgtcgggcg aaaagaggag
      781 gagctccgga attcccgcga gcagctccgt gccttggccc gtcacctgca atccgttcgg
      841 gaggaggagc gttcccggat cgcccgagag atccacgaca ccctgggaca gccgctggcc
      901 gggttgaagc tcgacctcgc gtggttggcg gggcggctgc cccccgacca ggcgccgctg
      961 ctggagaagc tgaggacgac gctggccctt gccgattcca cgatccggtc ggtccgacag
     1021 atcacgacag atctacgccc cagcctgctg gatgacctcg gcctggtggc cgccatcgaa
     1081 tggctggcac aagatttcca gacgcgcacc ggcatcggct gtgagtttgc ctctgaccag
     1141 ccggacctct tgcttccctc cgagctaagc acggcggtct tccgcatctg ccaggaatcc
     1201 ctgaccaacg tcgcccgaca cgcccacgcc acccgggtcc gtctggccct aaaggccgag
     1261 gcgggccacc tgcttctcac cgtcgccgac aacggtcggg gcatcaccga gcaggagatc
     1321 gccagtcaga cctccgtcgg gctcttgggc atccgcgagt gggccctcct cctgggcggc
     1381 gaggccaact tcatgggaca gccaggagaa ggcacgacgg tgatagtgcg gatcccattg
     1441 aaggcgcccc gcgggggagg caactattga aacggcgtat tgggaatgac ccgccgccgc
     1501 ttcccggacc cgccccccag aaccggcgat caggccaaag atacgatcct ccaagcatgt
     1561 ctcgccattt ccttgacttc cctcaaagaa cctgactagt atcccgctca tcgtccatcg
     1621 caggtcggaa tccgacattc gaggctgtgt ggagctgtct cctgcctgat ggttgggttg
     1681 acttgcgctg cccaattgag aaagggggtg accgggaaga ccggggcagt gcctgtgcct
     1741 attgtcgagg tgggtggggt gagcgcctta cataacctgt gaaccgggag gttcctggga
     1801 gggtgcgaac atgggtggtg tagcgattct gctgacgatt gtggcgg
//
LOCUS       DBNGZC010000563         3346 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2396898, whole genome shotgun sequence.
ACCESSION   DBNGZC010000563 DBNGZC010000000
VERSION     DBNGZC010000563.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2396898"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="AC16G0_09805"
     CDS             complement(<1..184)
                     /locus_tag="AC16G0_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099945.1"
                     /translation="MTNDQFPNPNRAAIPVGNVVRRHSDRMLIWSLGFCHFPRLFASL
                     LKNELGMKVGGNTTRCR"
     gene            complement(200..1096)
                     /locus_tag="AC16G0_09810"
     CDS             complement(200..1096)
                     /locus_tag="AC16G0_09810"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR5099946.1"
                     /translation="MSDQAPGAQALADLQPRHQFFVGIDSDGCAFDTMEIKHKECFCP
                     SAIKHWNLQPVSKYTREAWEFVNLYSKWRGINRWHALVMVFDLLRERPEVHARKVEVT
                     AATKVREFMASGKPLSNDGLKAYRAEHPDPELDQAWAWTTGVNASIADLVHGVPPFPY
                     VQESLELLQRQADVVVVSATPGEALLREWEEHDIAKYARVIAGQELGTKKQHLALAAK
                     GKYPSDRILMVGDAPGDMEAARANGALFFPINPGREEQSWERFYKEGLGRFMAREYAG
                     AYEAALVAEFEKLLPSVPPWKR"
     gene            1375..2427
                     /locus_tag="AC16G0_09815"
     CDS             1375..2427
                     /locus_tag="AC16G0_09815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR5099947.1"
                     /translation="MELRGCGTSDLKLSALGLGCWAFGGGAYWGDCDQQDVDAVVRRS
                     VELGINYFDAAEAYNDGRSEESLGRAVRGLPRDRFVIGTKVSPSNCYAETLPRHCEAS
                     LRRLGLDHLDLYMIHWPIHPHSIRHFSQDESVIQNPPTIQEGLGALARLKERGKIRYI
                     GVSNFGKDRLEEALRICPDIVVNQLPYSLLTRAIELESLPQCRSLGVGVIGYMTLLQG
                     VLADLYPTLEAVPPYQRRTRHFHSRTSHLARHGEEGAETETNAALADIRRIMQECGMT
                     MPEVATKWALAAHGIACSLIGVRSVRRLEDNVRAAAVPLPPEIVAKLNAVTRPVLDKL
                     GNSFDYYESFENNRTR"
     gene            2919..>3346
                     /gene="dctP"
                     /locus_tag="AC16G0_09820"
     CDS             2919..>3346
                     /gene="dctP"
                     /locus_tag="AC16G0_09820"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HIR5099948.1"
                     /translation="MRRLLQCIAIFAAVLAVALVLQPAEAKTKINIGSTYGPDAPVHF
                     GMVKFKELVEQRSKGDMEVQIHVGGAMGGERDVFEAMSSGGLEMGAMGSGDVSIFFPK
                     WMVYEVPYALRDADHFWKFWNGPVGKEVNDMVLKERGVMTT"
BASE COUNT          628 a         1063 c         1048 g          607 t
ORIGIN      
        1 tcctgcaacg agtcgtgttg ccacccactt tcatgcccag ctcattcttc agcaggctcg
       61 cgaataagcg ggggaaatga caaaatccca atgaccagat gagcatccga tcgctatgcc
      121 gccgaacgac attgccgacc gggatcgcgg cccggttggg atttgggaat tggtcattgg
      181 tcatttgctt ctgctcccgc taccgcttcc agggcggaac cgaaggcagg agcttctcga
      241 actctgccac cagagccgcc tcgtaggcgc ccgcgtactc gcgggccatg aacctgccca
      301 ggccctcttt gtagaaccgc tcccaggact gctcctcgcg acccggattg atggggaaga
      361 agagggcgcc gttggcgcgc gccgcctcca tgtcgccggg agcatcaccg accatcagga
      421 tgcggtccga ggggtacttg cccttcgccg ccagcgccag gtgctgcttc ttggtcccca
      481 gctcttgccc ggcgatgacg cgggcatact tggcgatgtc gtgctcttcc cactcgcgca
      541 ggagcgcctc cccgggtgtg gcgctcacca ccaccacgtc tgcctggcgc tgcaggagct
      601 ccaggctctc ctgcacgtac ggaaagggtg gcacgccgtg caccaagtcg gcgatggagg
      661 cattcacgcc ggtcgtccag gcccaggcct ggtccagctc gggatcggga tgctccgccc
      721 gataggcctt gaggccgtca ttgctcaacg gtttgcccga cgccatgaac tcacgcacct
      781 tggtcgcagc cgtcacctcc accttcctgg cgtgcacctc gggtcgctcg cgcaagaggt
      841 cgaagaccat gaccagggcg tgccagcggt tgatcccgcg ccacttcgag tagagattga
      901 cgaactccca ggcctcgcgg gtgtacttgg acaccggctg caggttccag tgcttgatcg
      961 cgctggggca aaaacactcc ttgtgcttga tctccatcgt gtcaaaggcg cagccgtccg
     1021 aatcgatccc cacgaagaac tggtgccgag gttgcaggtc ggccagcgcc tgcgcccctg
     1081 gagcctgatc gctcataata tcgtctcctc cttcggggat ttccgggaac cccgctgcag
     1141 accacgccac gcagggtgac aggaaggatt gaatgctgat acaccttcta ccaaaacagt
     1201 cgaaaggacg caagaccggg tataacggcg acgccggagg gggcatgggc cagcgccgat
     1261 tcgcgcggaa ccctgcgaaa gtccttgacc gtcatttctc actcgacgga tgatgggcag
     1321 cgtcacgacc atccggtatt ggcggaggcc actcgcggcc aggagggaga ctccgtggaa
     1381 ctcagaggct gcggcacgtc ggatctgaag ctgtcggcgc tgggtctggg atgctgggcc
     1441 ttcggcgggg gagcctactg gggcgactgc gatcagcagg atgtggatgc ggtggtccga
     1501 cgctcggtgg agctgggtat caattacttc gacgccgccg aggcatacaa cgacggccgg
     1561 agcgaggagt cgctgggccg ggcggtgcgg ggcttgccgc gcgaccgatt cgtgatcggg
     1621 acgaaggtgt cgccctcgaa ctgctacgcc gagaccctcc caaggcactg cgaggcctcg
     1681 ctccggcgcc tggggctcga ccacctggac ctctacatga tccactggcc gatccatccg
     1741 cactccatcc gccacttctc ccaggacgaa tcggtcatcc agaatccgcc gacgatccag
     1801 gaaggcctcg gcgcgctggc tcgtctgaaa gaacgaggga agatccgcta catcggcgtc
     1861 agcaacttcg gcaaggatcg gctggaagag gcgctcagaa tctgtccgga catcgtggtg
     1921 aaccaactgc cgtacagcct gctgacccgg gcgatcgagc tggagagtct gccgcagtgc
     1981 cggagcctgg gggtgggcgt catcggttac atgaccctgc tgcagggcgt gctcgcagat
     2041 ctttatccga cacttgaggc tgtaccgccc taccaacggc gcacccggca ctttcacagc
     2101 aggacctccc atctggcccg ccacggcgag gaaggggcgg aaaccgagac aaacgccgcg
     2161 ctggcggata tccgccggat catgcaggaa tgcggcatga ccatgcccga ggtcgccaca
     2221 aagtgggcgc tcgccgcgca cggaatcgcc tgctccctga tcggggtccg gagcgtccgg
     2281 aggctcgagg acaatgtccg tgccgcggcc gttccactcc cccccgagat cgttgcgaag
     2341 ctgaatgccg tcacgaggcc ggtcctggac aaactcggca acagctttga ctattacgag
     2401 agctttgaga acaaccgcac gcgctagcgg tcaaactacg tccaaggcgg gcccgaccct
     2461 agcggtccgg gccggcgaaa tccccccagt cttcccctga atagtccgct cattcagacc
     2521 tatccatccc cgcccacccg agtaaggctt tggtgttgac agcatccatg gccttcgata
     2581 gcctccccag cacgtccttc tgcgcaaggg ctcgttccga gtaagaggac gcccgaccat
     2641 cgtccgagcg tcgtcgatca ttcgacctag gtaccctagg gaatgcctct gagcaacccc
     2701 caacactgtc ggtccgcctc cgacccgtgg agagcaggag aaagtgtatc gttcatctcg
     2761 aagcctcttc gcttctcgct ccttgcctgt acacgcactg ctcaaggatc agcgagaaat
     2821 gattgggaaa acgtcggacc gtgcttttcg acgccgaagg ggagccattg ggtaacccgt
     2881 cgggtgagta tttatcacgt taacgaggga ggtctgccat gaggcggttg ctgcagtgca
     2941 tcgcgatctt cgcggcggtc ctggctgttg ctctggtgct ccagccggcg gaggccaaga
     3001 ccaagatcaa catcggctcc acctacggac cggatgcgcc ggtccatttt gggatggtga
     3061 agttcaaaga actggtggag cagcggagca agggcgacat ggaggtccag atccacgtcg
     3121 gtggcgccat ggggggcgag cgggacgtct tcgaggccat gagttcgggc ggcctggaga
     3181 tgggcgccat gggctcgggc gacgtctcta tcttcttccc caagtggatg gtgtacgagg
     3241 ttccctacgc cctgcgggac gccgaccact tctggaagtt ctggaacggc ccggtcggta
     3301 aagaggtcaa cgatatggtg ctcaaggagc ggggggtcat gaccac
//
LOCUS       DBNGZC010000564         3209 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_49740, whole genome shotgun sequence.
ACCESSION   DBNGZC010000564 DBNGZC010000000
VERSION     DBNGZC010000564.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_49740"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1390
                     /locus_tag="AC16G0_09825"
     CDS             107..1390
                     /locus_tag="AC16G0_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007264422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5099949.1"
                     /translation="MIWKLSASGFLGLLGFLTTALAACGGEQHSPSVEVAAVWTGAEQ
                     KAFAEVMAAFEKQTGARVTYTSTGDDIATILGTRIQGGNPPDVAILPQPGLLVDLARQ
                     DVLKPIQQVAGAEVDKHYAPIWRQLGSVNGILYGVWFKAANKSTVWYNVQVFGNAGVQ
                     PPANWAELLQAAKILSDYGVPPFSIAGADGWTLTDWFENVYLRTAGPVLYDRLTRHEI
                     PWTDPSVGVALSTLAQILGREEWLAGGTEGALQTDFPTSVTQTFRAPPRAAMVYEGDF
                     VAGVIAGETSARLGVVADFFPFPSIRSSPPSVVGGGDVAVLLRESEAGKALIRFLASP
                     EAGEIWARLGGFTSPSRGVSLSVYPDDVSRRSAEALLGAQVFRFDMSDQMPAAFGGTP
                     GQGEWAILQDFLRRPSDVRGTMQRLEAAAKQAYRR"
     gene            1404..2696
                     /locus_tag="AC16G0_09830"
     CDS             1404..2696
                     /locus_tag="AC16G0_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018890988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5099950.1"
                     /translation="MPPPLTLIRRSLLLTLFLGPALLLLGAIVLYPIGFTLVRSLYDR
                     SGEAFVGFANYRQMLALPEIDRVIRNNVIWVVVAPCAVTALGLVLAVLTERIRWRTAF
                     QVILFMPMAISFLSAGVIWRLVYQRDPSLGLANAVIRAGVNLFRPPGLYFGARPSMPG
                     LLSLEGDGFVSTRTYRPGDVAAFGLVAIPPSLLQNRAIRATPPRATAKSIQGVVWLDF
                     TLGGGGERGVIGPSEKGLPGVGVQALLGGRTVAVAVTGADGAFLLPDLPPGEYRLRLE
                     GASFRAPFDGIPWLGPILVTPAIILSYIWIWAGFAMVVIGAGLAALPREVLEACRVDG
                     ASEWQVFRQVTVPLLAPALAVVFVTLVINVLKIFDLILVIPPGSALADANVIALEMWQ
                     VSFGGIRDLGLGSALAVVLFLLVLPAMALNARRFGGGA"
     gene            2693..>3209
                     /locus_tag="AC16G0_09835"
     CDS             2693..>3209
                     /locus_tag="AC16G0_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5099951.1"
                     /translation="MTAAGLVRTLTRGPLNVLLLLAGTAWFTPTLGLLVSSLRTAAAN
                     SQSGWWVALWAPGELTLDSYRRLLQNPGSLRALLNTALITLPSTLLVVGVGALAAYAF
                     VWVRFPGRDRLFLLVVALLVVPLQVALIPVADLYNRLGLFGTILGAVLFHTAFGLPFA
                     VFLLRNFIREIP"
BASE COUNT          456 a         1059 c         1049 g          645 t
ORIGIN      
        1 accggcctgg tgaatggcat gtggtccaat ggtgttccgg cccctggagg catccccgga
       61 tgacgctcac acacccggaa gctccctctg tcgcagggct tcactcatga tatggaagct
      121 gagtgcgtcg ggatttctgg ggctcctggg cttcttgaca acggcgcttg cagcctgcgg
      181 cggtgagcag catagtccca gcgtcgaggt cgctgccgtg tggaccgggg cggaacagaa
      241 ggccttcgcc gaggtcatgg cggcctttga aaagcaaacc ggggccaggg tgacgtacac
      301 ctcaaccggg gacgacattg cgaccatcct gggaacccgt atccaaggtg gcaacccacc
      361 tgacgtggcc attctgccgc agccgggcct gttggtggac ctcgcccgac aggatgtcct
      421 caaacccatc cagcaggtcg cgggcgcgga agtggacaag cactacgcgc ccatctggcg
      481 acagcttggc agcgtcaacg ggatcctgta cggcgtgtgg ttcaaggccg caaacaagtc
      541 gacggtctgg tacaacgtgc aagttttcgg gaacgcgggc gttcagcctc cggcaaactg
      601 ggcggaactg ctccaagcgg ccaagatcct ctcggactac ggtgtgccgc cattttccat
      661 tgcgggcgca gatggatgga ccctcacaga ctggttcgag aatgtttacc tccggacggc
      721 cggtcccgtc ttgtatgatc ggctcacccg gcacgagatc ccctggacgg acccgagcgt
      781 cggggttgcc ctgagtacgc tggcccagat cctcggccgt gaagagtggc tcgccggcgg
      841 aaccgaaggg gccctgcaga cggacttccc gacctctgtc acccagacgt ttcgcgcccc
      901 gccgagggca gccatggttt acgagggtga ctttgtggcc ggcgtgattg ccggagagac
      961 cagcgcaaga cttggggtcg tcgccgactt cttccccttt ccatccatcc gcagctcccc
     1021 tccgagcgtg gtcggaggcg gcgacgtcgc ggtcctgctg cgggagagcg aggctggaaa
     1081 ggctctcatc cggtttctgg cctcgcccga ggctggggag atctgggccc ggctcggcgg
     1141 cttcacctcc cccagccggg gcgtgagctt gtccgtctac ccggacgatg tctcccgccg
     1201 atcggccgag gcgctcctcg gcgcacaggt cttccgattt gacatgtccg accagatgcc
     1261 cgccgcgttc ggggggactc ccggtcaggg ggagtgggcg atcctacagg atttcctgcg
     1321 tcgtccctca gacgtccgag ggaccatgca gcgtctggag gcggcggcga agcaagccta
     1381 caggcgctga ggagatcggg gcgatgccgc ctccgttgac gttgatccga cgatccctgc
     1441 tcctcaccct gtttctgggc cccgcgcttc tacttctggg cgccatcgtc ttgtacccga
     1501 tcggcttcac actggtcaga agtctctacg atcgatccgg cgaggctttc gtcgggttcg
     1561 ccaactaccg gcagatgctc gcccttcccg agatcgacag ggtgatccgg aataacgtca
     1621 tctgggttgt ggtcgccccc tgcgcggtga cggccttggg tctggtgctt gccgttctca
     1681 cggagcgcat ccggtggcga acagcctttc aggtgatcct gttcatgccg atggccatct
     1741 cgttcctgtc cgccggggtc atctggcgcc tcgtgtacca gcgcgatccc agcctggggc
     1801 tggcaaacgc cgtcattcga gccggggtga accttttccg gccacctggt ctgtacttcg
     1861 gggcccggcc atccatgccc ggcctcctct cgctggaggg cgacgggttc gtgagcacgc
     1921 gtacctacag accaggggat gtggccgcat tcggcctcgt cgcgatcccg ccgagcctcc
     1981 tgcagaaccg ggccatccgc gcaacccccc cgagagcgac ggcgaaatcg atccaagggg
     2041 tggtgtggtt ggacttcacc ctcggcggtg ggggggagcg cggcgtcatc gggccctcgg
     2101 agaaggggct tccgggcgtt ggggtccagg ctctactcgg cgggcgcacg gtcgccgtgg
     2161 cggtgacggg agccgatggt gcattcctcc tgccggacct tcctccaggg gagtatcggc
     2221 tgcggctgga gggtgcgagc tttcgtgcgc cattcgacgg catcccatgg ctcgggccca
     2281 tcctggtcac gccggcgatc atcctctcgt acatatggat ctgggcgggg ttcgccatgg
     2341 tcgtgatcgg cgccggcctg gccgcccttc ctcgggaggt tctcgaggcc tgtcgggtgg
     2401 acggcgcgag cgaatggcag gtcttccggc aggtgaccgt ccctctcctc gccccggccc
     2461 tcgccgtggt cttcgtcacg ctcgtcatca atgtcctcaa gatcttcgac ttgatccttg
     2521 tcatcccgcc ggggtcggcg ctggctgacg caaacgtgat cgccctggag atgtggcagg
     2581 tgtccttcgg gggaatccgg gatctgggcc tggggagcgc cctggcagtc gtcctgttcc
     2641 tcctcgtgct cccggctatg gcgctcaacg cccgacggtt tggagggggc gcatgacggc
     2701 cgcgggtctg gtacgcactc tcacccgggg cccgctgaac gtgctactcc tgctggccgg
     2761 gaccgcgtgg ttcaccccca cgctcggcct cctcgtttcc tcgctccgta ccgccgccgc
     2821 gaattcccag agtggttggt gggtcgcact ctgggcacct ggagagctca cgctagactc
     2881 ctaccgccgc ctgctgcaga atcccggaag tctccgcgcg cttctgaaca cggcgctcat
     2941 caccctgcca tccacacttc tggtggttgg cgtcggagcc ttggcagctt acgcgtttgt
     3001 ctgggtgcgc tttccgggcc gcgatcggct gttcctgctg gtggtggcac tgctggtcgt
     3061 tccgctgcag gtcgccctga tccccgtggc ggacctgtac aatcggctgg ggttgttcgg
     3121 aaccatcctc ggagccgtct tgttccacac ggccttcggg ctccccttcg ccgtcttcct
     3181 gcttcggaac ttcatccgtg agattccac
//
LOCUS       DBNGZC010000565         2221 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2396951, whole genome shotgun sequence.
ACCESSION   DBNGZC010000565 DBNGZC010000000
VERSION     DBNGZC010000565.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2396951"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..323
                     /locus_tag="AC16G0_09840"
     CDS             153..323
                     /locus_tag="AC16G0_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099952.1"
                     /translation="MTKRKIEYWVIPPDQDAEFVADMENVLETYAEAYDRHRPVLCMD
                     EQPVQLLRVQPV"
     gene            513..2060
                     /locus_tag="AC16G0_09845"
     CDS             513..2060
                     /locus_tag="AC16G0_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099953.1"
                     /translation="MSTGQASRPTVACPPPGEALPQLIHQRLDEIIAFCRNHPGPTSF
                     LEFERSLLGLLRSLGCLLIQLFLQARHRRLDRTPWTRTRGYRVADDAAERTLKTCCGE
                     VTYHRAYLLPRRGGGPGVHPLDVALGLTRDAYSPLVIGWFCRLATRVSFQAASRLGGM
                     FLGAAPPASAIEEWVLGLARPAYVYLSDGPLPEDDGEVLVIEVDGKAAPTATERELRR
                     RRGPRSRHRPGCQCQRHRGRAKRQRRGRRTRRKKGDKSKNGRSATLVVMYTLRRGEDG
                     QLHGPINKKVFGTFGSRRSALEWARVQATRRGFPPGTAKRVQIVVDGETCLEQRLRRL
                     FRGATLTLDIRHAQEKLWEVGRLFHREGSAELTHWVEDLEALLYKGHVRTLLRRLEQD
                     LRGVGRHGPGTKAKREVLTQAIGYLGARVKLMDYGRLRKEDLVIATGVVEGAARYVVG
                     ERLDNSGMRWIEARAEAVLLLRCIEVNGEWETFMRWSQERRSQELERGEVVQIRSKTP
                     IQLPRAA"
BASE COUNT          411 a          636 c          790 g          384 t
ORIGIN      
        1 ggcgaagcga agctgatcgc gatgcggttg gggaagccgc cagcggggta cggtcgttgg
       61 acgttgcagt tactggccga tgaactggtg ttgttggaag tggtcccttc gatctcggcg
      121 gagacggtcc gcaaggtgct caaaaaaatg gcatgaccaa gcgcaagatc gagtactggg
      181 tgattccgcc cgaccaggat gccgagttcg tggctgacat ggaaaacgtg ctcgaaacct
      241 atgcggaagc gtacgatcgg catcgaccgg tgctctgcat ggacgagcaa ccggtccaat
      301 tgctgagggt gcagcccgtt tgactgcaaa cctcttgtga tcgtaaatcc ttgaactgcc
      361 gcaggaaagc actcgctcga tggagcgggc ctcgtccgga ggcgtgctcg aaggaccatt
      421 attcgcggcg catcggtgaa ggtgaggctc aggggacgta tcctgaccgc tgatcaacag
      481 ccccaaacgg gaacgatcag gaggccgtcc ccatgagcac cggccaggct agccggccca
      541 ccgtcgcgtg tccaccgcca ggcgaagcat tgccccagtt gatccatcag cgactcgacg
      601 agatcatcgc gttctgtcgg aatcatcccg gtcccacgtc cttccttgag ttcgagagat
      661 ccctcctggg gctgcttcga tcgctgggct gcctgctgat ccaactgttc ctccaggcac
      721 gccatcgccg gctcgaccgg acgccctgga cccggacacg tggctatcgt gtggcggacg
      781 atgccgccga gcggacgctc aagacctgtt gtggggaggt cacctatcat cgggcctacc
      841 tcctgccacg ccgcggaggt ggccccggcg tgcatcccct ggatgtcgcc ctgggcctga
      901 cgcgcgatgc ttacagtccg ctggtcatcg gctggttctg tcggctagcc acccgggtga
      961 gcttccaggc cgccagccgg ctcgggggga tgttcctggg agccgcacca ccggcctcgg
     1021 cgatcgagga gtgggtcttg ggcctggccc ggcccgccta cgtctacctg agtgacggcc
     1081 cgttgcccga ggacgacggc gaggtgctgg tcatcgaggt cgacggcaag gccgccccca
     1141 cagccaccga gcgggagttg aggcggcggc gtggcccgcg gtcccgccac cgaccaggct
     1201 gccagtgcca gcggcatcga ggccgggcga aacgacaacg gcggggccga aggacgaggc
     1261 gcaagaaggg ggataagagc aagaacggcc gcagcgcgac cctggtggtg atgtatacct
     1321 tgcggcgggg tgaggacggc cagctgcacg gcccgatcaa caagaaggtg tttgggacct
     1381 tcggctcgcg gcgatcggcc ctggagtggg cgcgggtgca ggcgacgcgg cgcggtttcc
     1441 cccccgggac ggccaagagg gtgcagatcg tggtcgacgg cgagacctgt ctggagcagc
     1501 gcctgcggcg gctgtttcgc ggcgcgaccc tgacgttgga catccggcat gcgcaggaga
     1561 agctctggga ggtgggccgg ctgttccacc gggaggggag tgcggagttg acacactggg
     1621 ttgaggacct ggaagcgttg ctctacaagg ggcatgtgcg gacgttgctg cggcgattgg
     1681 agcaggatct gcgaggcgtg ggtcggcatg gccccgggac gaaggcgaaa cgagaggtcc
     1741 tcacacaggc catcggctac ctgggggcac gggtgaagct gatggattac gggcggttgc
     1801 ggaaggagga cctggtgatt gccacggggg tggtcgaagg cgcggcgcgg tacgtggtgg
     1861 gagagcggct ggataacagc gggatgaggt ggatcgaggc tcgtgcggag gcggtgttgc
     1921 tgctacggtg tatcgaggtc aacggcgaat gggagacctt catgaggtgg agccaagagc
     1981 gacgcagcca agaacttgag cgtggtgagg tcgttcagat ccggagcaag acccccatcc
     2041 aactccccag agcagcatga gcaagaacgg cttgcagcca aacggtctgc acccatcctg
     2101 tttgagagtc tggcgattcg ttctctgatc atctttatcc cgcacgcttc tcatcaaagt
     2161 ggatcaggtt ggaaggcctg atccggaatc tgaaatctcg atacgagtta ctcggcgatt
     2221 a
//
LOCUS       DBNGZC010000566         2315 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2004301, whole genome shotgun sequence.
ACCESSION   DBNGZC010000566 DBNGZC010000000
VERSION     DBNGZC010000566.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2004301"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..374
                     /locus_tag="AC16G0_09850"
                     /pseudo
     CDS             45..374
                     /locus_tag="AC16G0_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012372.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            325..957
                     /locus_tag="AC16G0_09855"
     CDS             325..957
                     /locus_tag="AC16G0_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012372.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HIR5099954.1"
                     /translation="MSGERSGLWWEYLRIVDLFRPVGVLVENVFSGLSLWHSTVRCSL
                     EGLDYRTASARIDAADVGAPHGRPRVFVLAYSGSKREPQPETLVCEDREWAIDFCSPL
                     ADADGKRLAGPIRVTESARRRASVDGRQEARGSAEPRVGRDAHGRAARMDAHRWPFGQ
                     GEEQHEGEPAGTVRSCKDRKDRIKSLGNAVTPQQGLAAMRWALGILGWVS"
     gene            1533..1820
                     /locus_tag="AC16G0_09860"
     CDS             1533..1820
                     /locus_tag="AC16G0_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099955.1"
                     /translation="MSRTVLEIKIVVSQATIGEDGKVDRGHRHAVKVYAETATFPSWT
                     LTAGTARGLGREAVKMVLDRDNMLVQTFEGEVTFPDTDLVLRGAPNPSTPA"
     gene            1833..2060
                     /locus_tag="AC16G0_09865"
     CDS             1833..2060
                     /locus_tag="AC16G0_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099956.1"
                     /translation="MNKNVLVITIKAEWRGPPGALDGFLPPSPQASAAVRAEVPWEPV
                     ANMVQGRLAIMCPGLTVTVEPQTEGPASPRQ"
     gene            2057..>2315
                     /locus_tag="AC16G0_09870"
     CDS             2057..>2315
                     /locus_tag="AC16G0_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099957.1"
                     /translation="MTPGRPPGDPKDPEGLLDVLARLVATGSPWAVEVEQWRQGNPGR
                     ALTEGHRRVLRQERDRTGPRPKLTAADEADVQAIVEAYVAGQ"
BASE COUNT          456 a          663 c          818 g          378 t
ORIGIN      
        1 gaacgggcgc ttgcccgtga gcatctcgta gagcaccact tgaggaggct ggtctcaatc
       61 ctcggcttgc tgcgcaggta gagattgatc cgttctgtcg gcgcgttctg gagaagcact
      121 ggctgactgt gtaccgtgac gtgaaggacg tgcgcgatgt cgatcgagaa aacaccccct
      181 gggccgacct catcgtcggc gggtttcctt gcgccgactt gtcagcggct ggacatcagg
      241 cgtggcgtag ggcaattgcc cagttctggc agatcgtgcc tacgtgcgcc ggcggcctcc
      301 ctcgcccagg cgatcgcgtc ggggttgtcg ggggagcgtt ctggcctttg gtgggagtat
      361 cttcggatcg ttgatctgtt cagacctgtc ggcgtccttg tcgaaaacgt tttctccggc
      421 ttgtctctgt ggcacagcac ggttcgttgc tcgctcgaag gattggacta tcgaacggcc
      481 agcgcaagaa tcgacgcggc ggacgtcggg gcgccgcatg gacgcccaag ggtatttgta
      541 ctggcctact ccggtagcaa gcgggaacca caaccggaaa cgcttgtctg tgaagatcgg
      601 gaatgggcta tcgacttctg ttcgccgttg gccgacgccg acggcaagcg actggcgggg
      661 ccgatccgag tcaccgaatc ggcaaggcgg cgtgcctctg tcgatggccg tcaggaagca
      721 aggggaagcg ctgaacccag agtgggtaga gatgctcatg ggcgcgccgc tcggatggac
      781 gctcatcgct ggccattcgg acaaggcgaa gagcagcacg agggcgaacc ggcggggacc
      841 gtcaggtcgt gcaaggatcg aaaagataga atcaaatcac tagggaatgc cgtaacgcct
      901 cagcagggac tcgcggcgat gcggtgggcg cttggaattt tggggtgggt ctcatgactt
      961 gggtgcgcgt cgacgacggg atgcctcgcc atccaaagtt catcgagctt gacgacggca
     1021 ggacgcctga gcaggctggc atgattctgc tcccggaacc agggggggcg tgatggtctg
     1081 gttcaaggtg gacgaccgga tagcatttca caggaaggcc atcagggccg ggaatgaagc
     1141 cattggggca tgggtgcgcg cgggggcgtg gtcgtcggcg ccggagaacc tcacggaggg
     1201 aaggattccc gtcgaagtgg cccacctgat cgcgccgctg aaaggtaggg agcggctgcg
     1261 cgccgctggg ttcgtcgagc cacaggacgg cagcgaagga taccagattc acgattttct
     1321 taattacaat ccatcggcgg agaaggttcg gcagcagcag gaggcgcaca gggagcgcac
     1381 gagacgatgg cgtgacggaa agcgtgacgc gtcaccatca ccgtcacgtg acgcggagga
     1441 gacgtctgat cccgtacccg tacccgatct ctcctccccc cactctcaaa gcaaatccgg
     1501 gatcgcgcac gaagagcaag gagaatgcca agatgagcag aacagtgctg gaaataaaga
     1561 tagtggtgag ccaggctacg atcggcgagg acggcaaggt ggacaggggc catcgtcacg
     1621 cggtgaaagt ctacgccgag acggcaacgt tcccgagttg gactctgacg gcaggcacgg
     1681 cgcggggact cggccgggaa gccgtcaaaa tggtcctcga ccgggacaac atgttagttc
     1741 agacgttcga gggcgaggta acgttcccgg acacggatct ggtccttcgc ggggcgccga
     1801 acccttcgac cccggcatga caggaggcga cgatgaacaa gaacgtgttg gtgattacaa
     1861 tcaaggcgga gtggaggggc ccgcccggcg ctctggatgg tttcctgccg ccgagcccac
     1921 aggcatcggc ggcggtgcgg gcggaggtcc cctgggaacc ggtggcgaac atggtgcagg
     1981 gacggctggc gatcatgtgc cccggcctaa ccgtcacggt cgagccccag acggagggcc
     2041 ctgccagccc gcgccagtga ccccaggacg gcctcccggg gaccccaagg acccggaagg
     2101 cctcctcgac gtcctggcgc gcctggtggc caccgggagt ccttgggcgg tggaggtcga
     2161 gcagtggcgc cagggcaacc caggccgagc cctgaccgag ggccaccgga gggtcttgcg
     2221 ccaggaacgc gaccggaccg gcccacggcc caaactcacg gccgccgacg aggcagacgt
     2281 gcaagccatc gtggaggcgt acgtcgccgg ccaga
//
LOCUS       DBNGZC010000567         3573 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2104357, whole genome shotgun sequence.
ACCESSION   DBNGZC010000567 DBNGZC010000000
VERSION     DBNGZC010000567.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2104357"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="AC16G0_09875"
     CDS             <1..246
                     /locus_tag="AC16G0_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099958.1"
                     /translation="HILVLGDVGSLSQGWLPGKGENRMVLALRGICGFRTQNLSGCTD
                     GQTDLSRISSIWENHWENKKRLQEKKFTSLPNHVIFS"
     gene            534..1754
                     /locus_tag="AC16G0_09880"
     CDS             534..1754
                     /locus_tag="AC16G0_09880"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR5099959.1"
                     /translation="MARYSIRIGTPTGSIQTLEADGESFEEARRSLEAKGYFVFDGGG
                     ERQGWTWTLPRIPKIRLDRIRPRTLLVFNQELLALVKAGLPIIVTLDLLRERSQQPRL
                     RALLGEVREAVKGGAALSAAMAKHPRIFSALYTAALHAGEQSGNFVDALSRYVTYQQR
                     ILALRQRFRAALTYPAILCVASLCVITFLLTFVVPTFTRIYGDMDQELPVATRILVSF
                     TGELQKTLPFAAAGIALFVLAIWRWRQTAAGRMLMDRAVMRFPFVGAMVRGYLYSRLS
                     RTLAMMLGGGIPMIPSLETALTIVGNTYMIDGIRRAIPRVAAGTSLANAFEASGVIPP
                     LMLELVGVGEKSGSLVEMLGHIAELYDGEVDTRLATLAATIEPAVMVVMGGIVATIVI
                     VMYLPIFHLASVVR"
     gene            1843..3549
                     /locus_tag="AC16G0_09885"
     CDS             1843..3549
                     /locus_tag="AC16G0_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HIR5099960.1"
                     /translation="MTRYRSLEEALVGEGLLPRPELEDILSQRDGMRGSLGWHLVDTG
                     RLTEDALAQALARLHDFPTVDLAAEIIEPGVLNAFPVERMRRDLFIPLRREGDRLVVA
                     MADPRNVLLRDDLAQWGGGPVDVVLATRSAIQDRLSRVDASQRVLNALGEDFKLADDE
                     GEEEGPVTLGLAPDEVSPTIRLVDTILLNALERHASDVHIESNERQVHVKYRVDGMLH
                     PAMEPMDRRHQDTIVSRIKIMAELDIAEHRIPQDGRFKLRAKGRSIDCRVSILPSNFG
                     EAVVIRILDKEAITKELAELRLDILGISKGDLRRFRRHIHRPHGMVLVTGPTGSGKTT
                     TLYAAITEIHTGDDKIITIEDPIEYQLQGVVQIPVNEKKGLTFARGLRSILRHDPDKI
                     LVGEIRDTETAQIAVQSALTGHLVFTTVHANNVMDVLGRFLNMGLEPYHFLSSLNCIL
                     TQRLVRVLCRACRKPVRYGREDLEESKLDPVLDANRTFFEPGGCEECHGSGFRGRTAV
                     AELVEVNDPIRALILDRRPATEITAGAQAGGTVVLRDAALAKARAGITTLAEINRVTF
                     AE"
BASE COUNT          673 a         1097 c         1134 g          669 t
ORIGIN      
        1 cacatccttg tcctcggcga tgtcgggtct ctttcccaag ggtggcttcc gggcaaggga
       61 gaaaatcgaa tggtgctggc tctgagggga atatgcgggt ttcggacaca gaatctcagt
      121 ggatgtacgg atggacagac cgatttgtcc aggatatctt caatatggga gaatcattgg
      181 gagaacaaaa agcgcctgca agagaagaaa ttcacttcac taccgaacca cgtgatattt
      241 tcgtgagcgc cgatgccagc atttcgactg gaccaaatca tcttggaaga cttaggataa
      301 tccaaacgtc ttatcgggca tctagggcta tatgaacaac ctgatccctt gatgcgcgtg
      361 gtaccaatag gatcgggatg gcgggtggca tcctgtaggg tcacaccgaa atggcagagg
      421 caaaagaaga tgatatttgg gacacttcaa tgtacaattc tcatcatttc cttggactct
      481 gcctcgttgg ttgtctaaaa ttcgccccta acatgttttc ggggtgagga agcatggcgc
      541 ggtattctat cagaattggg accccgaccg ggtccatcca aaccctcgaa gcggacggcg
      601 agagcttcga ggaggcccgg cgtagcctgg aggccaaggg gtacttcgtc ttcgatggcg
      661 ggggtgagcg gcagggttgg acatggacgt tgccgcgcat cccgaagatc agattggacc
      721 gcatcaggcc acgaacgctc ttggtcttca accaggagtt gctggcactg gtcaaggccg
      781 gacttccgat catcgtcacc ctcgatctcc tgcgcgagcg ctcccagcag ccccgtttgc
      841 gagccttgtt gggcgaggtg cgcgaagctg tgaaaggtgg cgccgcccta tccgccgcca
      901 tggccaagca tcctcggatc ttttccgcct tatacacggc ggcactccac gccggcgagc
      961 agagcgggaa cttcgtggac gcgctctcgc gttatgtcac atatcagcag cggatcctgg
     1021 ccctgcgcca gcggttccga gcagccctca cctatccggc catcctttgc gtggcatcgc
     1081 tctgcgtcat cacgttccta ctgacctttg tggtgccgac ttttacccgg atttacggcg
     1141 acatggacca agaacttccc gtcgccacgc gcatcctggt gtcgttcacc ggagagctgc
     1201 aaaagacctt gccattcgca gccgccggta tagccttgtt tgtcctggcg atatggcgct
     1261 ggcgccagac ggcggccgga agaatgctga tggaccgggc ggttatgcgc ttcccgttcg
     1321 tgggggccat ggtcaggggc tacctttact cccgactgtc ccggaccttg gcgatgatgc
     1381 tgggcggggg aattcccatg attccttcgc tggagacggc cctcacgatc gtggggaata
     1441 cctacatgat agacggcatc cggcgggcca tcccccgggt ggccgcgggt accagcctgg
     1501 cgaatgcttt cgaggcctct ggagttatcc ctcccttgat gctggagctt gtaggggtcg
     1561 gggagaagag cggatcatta gtagagatgc tggggcacat cgccgaactc tacgacggcg
     1621 aggtggacac acggctcgcg acgctggcgg cgacgatcga gccggccgtc atggtcgtca
     1681 tggggggaat tgtggcgaca atcgtcatcg tcatgtacct cccgatcttt cacctggcat
     1741 ccgtggtccg ctaggtgcca caaggggcgt aagctcgtcc gggacggatg cgtgcccgaa
     1801 ggtgcgcatc ccagatttct gggcaggaat tcgaggttcg gcatgacacg ctaccgttcc
     1861 ctggaagaag ctttggtggg cgagggactc ttgccccgcc ccgagctgga ggacatcctc
     1921 agccagcggg acggcatgcg cggcagtctc ggctggcacc tggtggacac tgggcggctt
     1981 accgaagacg cccttgccca ggccttggcc cgcctgcacg atttccccac ggtggatctc
     2041 gcggccgaga tcatagagcc cggcgtgttg aacgccttcc ccgtggaacg gatgcggcgt
     2101 gacctcttca tccccctgcg ccgtgagggc gacaggcttg tggtggccat ggcggacccc
     2161 cggaacgtcc tcctccggga cgatctcgcc cagtggggtg ggggtccggt ggacgtggtc
     2221 ctggccactc gctccgcgat ccaggatcga ttgagccgcg tggacgcctc ccagcgggtg
     2281 ctgaacgccc tgggtgaaga cttcaagcta gccgatgacg agggggagga ggaggggccg
     2341 gtcacgctgg gattggctcc ggacgaagtc agccccacca tccgcctggt ggacaccatc
     2401 ctgctcaacg ctttggaacg gcacgccagc gacgtccaca tcgagagcaa cgagcggcag
     2461 gtacacgtga agtaccgtgt ggacggtatg ctgcatccgg ccatggagcc gatggaccgg
     2521 cgccaccagg acaccatcgt ctcccgcatc aagatcatgg ccgagctgga catcgccgag
     2581 catcgcatcc cccaggacgg ccgattcaag ctgagggcca agggccgctc catcgactgc
     2641 cgggtctcga tcctgcccag caacttcggc gaggcggtgg tgatccgcat cctggacaag
     2701 gaggccatca ccaaggagct ggcggagctc cggctggaca ttctgggcat cagcaaaggg
     2761 gatcttcgac gcttccggcg ccacatccac cgcccccacg ggatggtcct ggtcacgggg
     2821 cccacggggt cagggaagac gaccaccctg tacgcggcca tcacggagat tcacacgggc
     2881 gatgacaaga tcatcaccat cgaagacccc atcgagtacc agctccaggg cgtggtccag
     2941 atcccggtga acgagaagaa ggggctcacc ttcgcccgcg gcctccggag tatcctgcgc
     3001 cacgacccgg acaagatcct ggtgggcgag atccgcgata ccgagacagc ccagattgcc
     3061 gtccaatccg ccctcaccgg ccacctggtc ttcaccacgg tgcacgccaa caacgtcatg
     3121 gacgtcctgg gacgcttcct gaacatgggc ctggagccct accacttcct ctcctccctg
     3181 aactgcatcc tgacccagcg cctggtgcgg gtcctgtgcc gcgcctgccg caaacccgtg
     3241 cgctatgggc gggaggacct ggaggagtcc aagctggatc ccgtcctgga cgccaaccgc
     3301 accttcttcg agccgggcgg ttgtgaggag tgtcacggct cgggcttccg cggccgcacg
     3361 gcggtggccg agctggtgga ggtcaatgac ccgattcgcg ccctgatcct ggatcgccgc
     3421 ccagccaccg agatcaccgc tggcgcccag gcagggggca ccgtggtgct ccgggacgcc
     3481 gccctggcca aggcccgggc ggggatcacg accctggcgg agatcaaccg ggtgaccttt
     3541 gcggagtgat gcggggacga ggagttcgag gca
//
LOCUS       DBNGZC010000568         1604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_731903, whole genome shotgun sequence.
ACCESSION   DBNGZC010000568 DBNGZC010000000
VERSION     DBNGZC010000568.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_731903"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      65..137
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            240..479
                     /locus_tag="AC16G0_09890"
     CDS             240..479
                     /locus_tag="AC16G0_09890"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR5099961.1"
                     /translation="MWPIYERSAGVIPFHFDGNRVPTYLVIHSATVQNPQARWEFPKG
                     AIEAGEYPREAAAREFEEETGLVSWAFRDGFERSL"
     gene            489..860
                     /locus_tag="AC16G0_09895"
     CDS             489..860
                     /locus_tag="AC16G0_09895"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR5099962.1"
                     /translation="MCRCQKHFKTVIYFAAEVFDTATMTRSHEHMEDPLGRWCHWGSF
                     DETTRMLCHAKIRHLFAEVDSWLRNGNSAARVRHAYRRESAREADHFRPRGWARIWAD
                     TNNTIPAPGLVRQLGHPPAQA"
     gene            complement(1085..>1604)
                     /locus_tag="AC16G0_09900"
     CDS             complement(1085..>1604)
                     /locus_tag="AC16G0_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_410177318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HIR5099963.1"
                     /translation="PVACQKAYRVVVLRKELVVEKGQLWLFEPDRDFFYITNDRTTPA
                     SEVVFLANDRCDQENLIAQLKGGVKALAMPVGDLVSNWAYMVMAGLAWSLKAWSALVL
                     PEQGRWSEKYRAEKRSLLRMEFSTFCVALIQVPCQIVRTSRRIVYRLLSWNPWQCVFL
                     RLVERLHGRRLC"
BASE COUNT          311 a          568 c          452 g          273 t
ORIGIN      
        1 cgtccttgcg ccttgacggg gggcgaccgg ccatcaagaa tgaatgatga cggcggggag
       61 accccgcgcg acgggtgatg gagttcaccc accgctcaac cacctcgtac cgcgaggttg
      121 ataactccta ctcagggccc atcgccctcg gtaggggttt tttgttgcgc aagcctggcc
      181 cggtgcgtgg cacatcgccg cgcctcgatc gcagacctgg cgtttggagc tcggcgaata
      241 tgtggcccat ctacgagcgt tccgcaggcg tgatcccgtt tcactttgat ggaaaccggg
      301 tgcccacgta cctggtcatc cactcggcca ccgtgcaaaa cccccaggcg cgatgggaat
      361 tccccaaggg ggcaatcgag gcgggcgagt atcctcgcga ggcggccgct cgcgagttcg
      421 aggaggagac cggcctcgtc tcttgggcct tccgcgacgg attcgagcgg agcctgtagt
      481 acacatacat gtgtcgatgc cagaaacact ttaagacggt catctacttc gcggcggagg
      541 tcttcgacac cgcgaccatg acccggtccc acgagcacat ggaggacccc ctcggccgct
      601 ggtgccactg gggctcgttc gacgagacca cccgcatgct ctgccacgcg aagatccgtc
      661 atctcttcgc ggaggtcgat tcctggctcc gcaatggcaa ctcggccgca cgcgtgcgcc
      721 acgcttacag gagagaatcc gccagggaag cagatcattt ccggccgagg gggtgggcga
      781 ggatctgggc agataccaac aacacaatcc cggcaccggg gctcgtccga cagctcggcc
      841 atccccccgc acaagcctga ttgaccggga cgaaaaagca ccctaaccct caaccaagcg
      901 taatgtgagc ccccatccct ggtcctttcc tgccccggat ggtcacgtac cgtctccggg
      961 ccgggtcctc gtcacccggt tgcacacatc ccgatctcga tcgctcatca cgccaccgcc
     1021 tctcattggt atggggccaa cacggaccgg agcatccgga ctccggcttc agggcgccgg
     1081 ccggtcaaca caaccggcga ccgtgcaatc gctccaccaa ccggaggaat acacactgcc
     1141 acgggttcca cgacaagagc cgatacacga tccgtcggga tgtcctcacg atctggcacg
     1201 gcacctggat caaggccacg cagaacgtgc tgaactccat ccgcagcagc gaccgcttct
     1261 ccgcacgata cttctccgac caccgtccct gttcgggcaa caccagggcg ctccacgcct
     1321 tcaggctcca cgccaggccc gccatcacca tgtacgccca gttgctcacc aggtcgccca
     1381 ccggcatcgc cagcgccttc acacccccct tcaactgggc gatcaggttc tcctggtcac
     1441 aacggtcgtt ggccaggaac acgacctccg acgccggagt cgtccggtcg ttggtgatgt
     1501 aaaagaagtc ccggtccggc tcgaacagcc acaattgccc cttctccacg accaactcct
     1561 tgcggagcac gactacccga tacgccttct ggcaggcgac cggg
//
LOCUS       DBNGZC010000569         3101 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_146948, whole genome shotgun sequence.
ACCESSION   DBNGZC010000569 DBNGZC010000000
VERSION     DBNGZC010000569.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_146948"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="AC16G0_09905"
     CDS             <1..408
                     /locus_tag="AC16G0_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family ABC transporter permease"
                     /protein_id="HIR5099964.1"
                     /translation="QIDAMRALGVNHIKKLVVPRLLVTMLALPLLTVIADALGIFGGM
                     MISQYEFNVDYHQYYTSFTQYVRLADMLSGLGKTVFFGFIIAVVGCFNGLETRGGTEG
                     LGRATTGTVVTSALLVFVSDFFLTKFFWWLEGW"
     gene            456..1262
                     /locus_tag="AC16G0_09910"
     CDS             456..1262
                     /locus_tag="AC16G0_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009645433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099965.1"
                     /translation="MAQASIELRGLWKVFDGHEVLRGVDLAVRSGESLTIIGGSGTGK
                     SVLLRLIIGLLKPEAGQILLDGQDIVPFPERELLRVRRKVGMVFQGSALFDSLSVGEN
                     VAFALREHTRLGEAEIAARVREALDLVGLNDVERMDPAELSGGMRKRVAVARAIALPP
                     SILLYDEPTTGLDPGNVEKIVELILDLKTKLGVTSIVVTHDMPSAMKLSDRIAMLHQG
                     RIAAIGTPEEVQATHHSLIRDFIEGRPRHEGDAGPEVADRTVERTVLHER"
     gene            1285..2451
                     /locus_tag="AC16G0_09915"
     CDS             1285..2451
                     /locus_tag="AC16G0_09915"
                     /inference="COORDINATES: protein motif:HMM:NF014523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIR5099966.1"
                     /translation="MQLRIGALVLAAILLFMGFVLTIGQRSTLFQERYSLVATFASTE
                     GLAVGAPVRLAGLTVGNVTRISFGRDPRDRRITMTLSVERRVQERIRQDSLASIGTIG
                     LVGDKVLDITVGSHDTPELPPGGRLASVDPLDYSVLLQKGDRILDHVTRISASLDEFL
                     AGGEQTGKRNLNDALRSLRTTLVEVEKGQGLLHDIIYGKEGNDLLARVDRTVQSLEKT
                     AQAIDTEHGLLHALIYTPQEETLGKLDRAVGNLDDLLRQAKEGRGLLHALLYDQDGTQ
                     TLTRLNQASAELEGLVREAREGKGLIPSLFFDPAGRKALEDLLAATSGLRGTATDLQA
                     IVAKLRQGEGTLGGLLEDPTLYEDLSALLRGANRSTLLRSLIRSTREEGARQTR"
     gene            2566..2769
                     /locus_tag="AC16G0_09920"
     CDS             2566..2769
                     /locus_tag="AC16G0_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099967.1"
                     /translation="MAIHLVIHGHFYQPPRENPWTGRIERQASAAPFHDWNARITEEC
                     YRPNASSRVLDGKGHIQDIVNNY"
     gene            2830..>3101
                     /locus_tag="AC16G0_09925"
     CDS             2830..>3101
                     /locus_tag="AC16G0_09925"
                     /inference="COORDINATES: protein motif:HMM:NF015052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HIR5099968.1"
                     /translation="MVAALEAGDRSSRARLGHGNAIAQGYNHLILPLATRRDRETKIR
                     WGVCEFQQRFARPPEAMWLPEAAVDATTLELLVRAGMRYVILSPAQA"
BASE COUNT          540 a          986 c         1073 g          502 t
ORIGIN      
        1 cagatcgatg ccatgcgggc cctgggcgtc aaccacatca agaagctggt ggtcccccgg
       61 ctgctggtca ccatgctggc cctgcccttg ctcaccgtca tcgccgacgc cctggggatc
      121 ttcggtggca tgatgatctc ccagtacgag ttcaacgtgg actatcacca gtactacacc
      181 agtttcaccc agtacgtacg gctggcggat atgctgagcg gcctggggaa gacggtcttc
      241 tttggcttca tcatcgcggt ggtcggatgc ttcaacggtc tggagacccg gggcgggacc
      301 gaggggctgg gacgcgccac gacggggacg gtcgtgacct cggcgctcct ggtattcgtc
      361 tcggatttct tcctcaccaa gttcttctgg tggctggagg gctggtaatc ctgcagtcgg
      421 tgtccgccgg ggcgcggggt cccggagagt cgaacatggc ccaggcaagc atcgagctcc
      481 gcgggctctg gaaagttttc gacggccatg aggtccttcg aggcgtggac ttggccgtcc
      541 ggtcagggga atccctcacc atcatcggcg gcagcggcac cggcaagagc gtcctgctcc
      601 ggctgatcat cggcctgctg aagccggagg cgggtcagat cctcctggac ggccaggaca
      661 tcgttccgtt cccggagcgg gagttgctcc gcgtccgccg aaaggtgggg atggttttcc
      721 aggggtcggc cctcttcgac tccctgagcg tcggcgagaa tgtggccttc gccctgcgcg
      781 agcacacccg tctcggcgag gcggagatcg ccgcccgggt gcgagaggcc ctcgacctgg
      841 tgggtctgaa cgacgtggaa cgcatggacc cggcggagtt gtctggtggg atgcggaaac
      901 gggtggccgt ggcgcgggca atcgcgctgc ctcccagcat cctgctctat gacgagccta
      961 ccaccggcct ggatcccggc aacgtggaga agatcgtcga gttgatcctg gatctcaaga
     1021 ccaagctggg ggtcacgtcc atcgtggtga cccacgatat gcccagcgcc atgaagctgt
     1081 ccgatcggat cgccatgctc caccagggac gcatcgcggc catcggcacg cccgaggagg
     1141 tccaggcgac ccatcactcc ttgatccggg acttcatcga ggggcgccct cggcacgagg
     1201 gcgacgcggg gccggaggtg gcagacagga cggtggagcg gacggtgctc cacgagaggt
     1261 gaaacgagga tgaccgagcg gcagatgcaa ctacgaattg gggcgctggt cctggcggcc
     1321 atcctgctct tcatgggatt cgtgctgacc atcggccagc ggagcaccct cttccaggag
     1381 cggtactccc tggtcgccac cttcgcctcc accgagggcc tggcagtcgg ggcgcccgtg
     1441 aggctggcag gcttgaccgt ggggaacgtc acccggatca gctttggtcg cgatcccagg
     1501 gatcgtcgca tcaccatgac cctgagcgtg gaacggcggg ttcaggagcg catccgccag
     1561 gattcgctgg cgagcatcgg gacgatcggc ctcgtggggg acaaggttct ggacatcacc
     1621 gtgggcagcc acgacacacc cgagcttccg cccgggggga gactcgccag cgtggacccg
     1681 ctggactact ccgtgctgct ccagaagggc gaccgaatcc tggaccacgt gacgcggatc
     1741 agcgcctcgc tggacgagtt cctggcggga ggggagcaga cggggaagcg gaacctgaac
     1801 gatgcgctcc gctcgctgcg gaccaccctg gtcgaggtgg agaagggcca gggactgctg
     1861 catgacatca tctacgggaa ggaaggcaac gacctgctgg cgcgcgtgga ccgcaccgtc
     1921 cagtccctgg agaagacggc tcaggccatc gacacggagc acggcctgct gcacgccctg
     1981 atctacacgc cccaggagga aacgctgggg aagctggacc gggcggtggg gaacctggac
     2041 gacctcttgc gccaggcgaa ggaaggccgg ggcctcctgc acgctctgct gtatgaccag
     2101 gacgggacgc agaccctcac ccgtctcaat caggccagcg cggagctgga gggcctggtg
     2161 cgggaggccc gggagggcaa gggtctgatc ccctcgctct tcttcgaccc tgcaggccgg
     2221 aaggccctgg aggacctcct ggcagcgacg tcgggtctgc gggggacggc gaccgatctg
     2281 caggccatcg tggccaagct ccgacagggg gaggggacct tgggtggcct gctggaggac
     2341 cccacgctgt acgaggacct ctcggcgctg ctccggggcg ccaaccggag cacgctgttg
     2401 cgcagcctca tccgctccac gcgggaggag ggcgcccgcc agacgcggta ggccggagca
     2461 atgcaaaacc gacaaccgac aacggacaat gccaaattga gaacgggcga aggattatcg
     2521 gcaattgtcg gttgtcagtt tgaatatttt tattgagcga agcgtatggc cattcatcta
     2581 gtcatccacg gacatttcta ccagccgccg cgcgagaatc cctggaccgg gcggatcgag
     2641 cggcaggcga gcgccgcgcc tttccacgac tggaacgcgc ggatcacgga ggagtgctac
     2701 cggccgaacg cctcctcgcg cgtcctggac gggaagggcc acatccagga catcgtcaac
     2761 aactactagc ggatcagctt caacgtcgga cccaccctct tctcctggct gggggaggag
     2821 gcgccggacg tggtggctgc cctggaggcc ggcgaccgga gcagccgcgc ccgcctcggg
     2881 cacgggaacg ccatcgccca agggtacaac cacctgatcc tgcctctggc cacgcggcgg
     2941 gatcgtgaga ccaagatccg ctggggcgtc tgcgagttcc agcagcgctt cgcccgcccg
     3001 cccgaggcca tgtggctgcc cgaggcggcc gtggacgcca cgacgctgga actcctggtc
     3061 cgggccggca tgcgctacgt gattttgtcg ccggcccagg c
//
LOCUS       DBNGZC010000570         6857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2397172, whole genome shotgun sequence.
ACCESSION   DBNGZC010000570 DBNGZC010000000
VERSION     DBNGZC010000570.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2397172"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..162)
                     /locus_tag="AC16G0_09930"
     CDS             complement(<1..162)
                     /locus_tag="AC16G0_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HIR5099969.1"
                     /translation="MSDLRELYQQVILDHYKKPRNFQKLPAANRRAEGHNPLCGDEIT
                     VYLQMEGDVI"
     gene            complement(162..1382)
                     /locus_tag="AC16G0_09935"
     CDS             complement(162..1382)
                     /locus_tag="AC16G0_09935"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020249435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HIR5099970.1"
                     /translation="MGFGVEKIREDFPVLQTKVHGKPLVYLDNAATTQKPRVVIEALT
                     RYYTEENSNIHRGVHFLSQVATQAYESGRTRVRQLLNAAHDHEIIFTRGTTEGINLVA
                     QCYGRSRLRAGDEVIISAMEHHSNIVPWQMICDEKGAILRVIPMNDAGELLLEEYERL
                     LGDRSKLVSVAYVSNALGTVNPIRQIIELAHRRGIPVLVDGAQASPHMPVDVRALDCD
                     FYAFSGHKLYGPTGIGVLYGKAELLEAMPPYQGGGDMISAVTFEKTVYNTLPHKFEAG
                     TPHIAGVIGLAAAIEYVHRIGLAQIAAHEADLLAYGTELLSAVPGLRIIGTAREKAGV
                     LSFVLDGIHAHDVGTILDLEGVAVRAGHHCAMPVMKRYGVPATARASLALYNTRADID
                     ALVKAIHKVIEVFA"
     gene            complement(1430..2773)
                     /gene="sufD"
                     /locus_tag="AC16G0_09940"
     CDS             complement(1430..2773)
                     /gene="sufD"
                     /locus_tag="AC16G0_09940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HIR5099971.1"
                     /translation="MTLTQAQETHTYGSHFEELVRSIPQSQSWFTPVRKAAVLRFSEV
                     GFPTVRDEDWRFTSVAPIAQIDFQPAPDAQTAVTLDTLGPFLVPGLPCMRLVFVNGRF
                     VPKLSSMGALPKGLRVSSLGRAMAEDRHALEPHLARYADIHRDPFLALNTACMEDGAF
                     ISIPRSMVLEEPVHLLYVSTDLTVPTVTHPRNLIVSGEDSQATVLEDYVSLGGGVAFS
                     NAITELVVGQNSVVSHYLIERENTRSFNVSTLRVEQGRSSNVTSHSVLLGGALVRNNI
                     HPVLAGEGCECMINGLFIPTGRQHMDNYMQVEHASPHGSSRQFYKGILDGQARGVFHG
                     RIIVHKDAQKTDAKQTNMNLLLSEDAQIDTKPQLEIYADDVKCTHGATIGQIDDEAIF
                     YLRSRGIAEASAKALLLFAFAGESLQRMKIEPLRKYLEGLVTQWLPQARPLEDVR"
     gene            complement(2791..3561)
                     /gene="sufC"
                     /locus_tag="AC16G0_09945"
     CDS             complement(2791..3561)
                     /gene="sufC"
                     /locus_tag="AC16G0_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013219706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIR5099972.1"
                     /translation="MLEIKNLHAKVGNREILRGVNLRINAGEVHSIMGPNGSGKSTLA
                     QVLAGRETYEVTAGDVLYHGKNLLEMAPEERACEGVFMAFQYPVEIPGVNNVYFLKAA
                     VNAVRKYRGEDEFDAMDFLALVKDKMKLLEMDDRLLNRSVNEGFSGGEKKRNEIFQMA
                     VLQPQFAILDETDSGLDIDALRIVANGVNALRAPERAILVVTHYQRLLNYIVPDFVHV
                     LVDGRIVKSGGKELALELEEKGYAWVENEARLTAPATL"
     gene            complement(3652..5109)
                     /gene="sufB"
                     /locus_tag="AC16G0_09950"
     CDS             complement(3652..5109)
                     /gene="sufB"
                     /locus_tag="AC16G0_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIR5099973.1"
                     /translation="MSTSTNAIEALANQEYKYGFVTDIDADAAPRGLSEDIIRLISAK
                     KKEPDFMLQWRLRAYRHWEKMVRSEGEPRWANVKFPPIDYQDIIYYSAPKSKKPLKSL
                     DEVAPELLRTFEKLGIPLQEQKQLAGVAVDAVFDSVSVATTFQKKLAELGIIFGSFSE
                     AVQNHPDLVQKYLGSVVPYTDNFYAALNSAVFSDGSFCYIPKGVRCPMELSTYFRINA
                     LNTGQFERTLIIADEGAYVSYLEGCTAPMRDTNQLHAAVVELVAHDDATIKYSTIQNW
                     YPGDKEGKGGIYNFVTKRGKCLGRNSHISWTQVETGSSITWKYPSCILQGDNSVGEFY
                     SVALTNNYQQVDTGTKMIHIGKNTKSTIVSKGISAGHGQNTYRGMVKILKGAAGARNY
                     TQCDSLLIGDKCGAHTFPYIEVKNATARMEHEASTSKIGEDQIFYCNQRAISKEDAVS
                     LIVGGFCKQVFKELPMEFAVEAQKLLSVSLEGSVG"
     gene            complement(5183..6265)
                     /locus_tag="AC16G0_09955"
     CDS             complement(5183..6265)
                     /locus_tag="AC16G0_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HIR5099974.1"
                     /translation="MVTEQAILAALATIKDPDLHQDIVSLGFVKGLKIDGGKVSFMIE
                     LTTPACPVKHQMEEGARRVVSALPGVQAVEVTMTSRVTTSRAPHSEYLPGVRNTIAVA
                     SGKGGVGKSTVAANLAVALMRSGARVGLMDTDVYGPCIPTLMGANGELEHGTNGKIIP
                     PILHGVKIMSMGFFLPKSEAVIWRGPMLHKMIQEFLGRVDWGELDYLVMDLPPGTGDV
                     QLSLCQSVPLTGAVIVSTPQDVALQVASKAILMFNKMKVPILGIVENMSYHTCSHCGQ
                     RDDIFGHGGAREASRQSEIPFLGEIPLDAGIRVKSDGGTPVALEGTEVPVAAAFHEIA
                     ARLAAQISILNAAGLAQNQEPVVVMR"
     gene            complement(6333..6737)
                     /locus_tag="AC16G0_09960"
     CDS             complement(6333..6737)
                     /locus_tag="AC16G0_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5099975.1"
                     /translation="MDGLKSMHVIVCSGNPAVVDAASRAFDHREHRHTVCESGLEVLG
                     VVGVLHSDLLVLDLETPGLGGLLLISAIRELAPDLPILAVSTKPVPDARALAQKGVPF
                     SLLGGSPGNETASLLATLAQVERKAAIGTGRS"
BASE COUNT         1172 a         2194 c         2114 g         1377 t
ORIGIN      
        1 gatcacgtca ccctccatct ggaggtagac ggtgatctcg tccccgcaca gggggttgtg
       61 cccctcggcc cgccggttcg cggccggaag cttctggaaa ttccggggct tcttgtagtg
      121 atccaggatg acttgctgat acagctcacg caggtccgac atcaggcgaa gacctcgatg
      181 accttatgga tggccttgac cagggcgtcg atgtccgcac gcgtgttgta gagcgccaga
      241 gacgcacgcg ctgtggccgg caccccatac cgcttcatga cgggcatggc gcagtggtgg
      301 cccgcccgga ccgctacccc ctctaggtcc aggatggtgc ccacgtcgtg ggcgtggatg
      361 ccgtccagca caaaggatag gacaccggcc ttctccctgg ccgtgccgat gatgcgtagg
      421 ccggggacgg ccgacagcag ttcggtgccg tacgccagga gatcggcctc gtgtgccgca
      481 atctgggcga ggccgatccg atgcacgtac tcgatggcgg cggcgagacc gatgacgccg
      541 gcgatgtggg gcgtgccggc ctcgaacttg tggggcagcg tgttgtacac cgtcttctca
      601 aaggtcacgg cgctgatcat gtccccgccc ccctggtacg ggggcatggc ctccagcagc
      661 tcggccttcc cgtagaggac gccgatcccc gtggggccgt acagcttgtg accggaaaag
      721 gcgtagaagt cgcaatccag cgcccggaca tccaccggca tgtgggggga ggcctgcgcg
      781 ccgtccacca gcaccgggat gccgcgtcgg tgcgccaatt cgatgatctg ccggatcggg
      841 ttcaccgtcc ccagggcgtt ggagacatag gccaccgaca ccagcttgct gcgatccccg
      901 agcagtcgct cgtactcctc gagcagcagc tctccggcgt cgttcatggg gatgacgcgg
      961 aggatcgccc ccttctcatc gcagatcatt tgccacggaa cgatgttcga atgatgttcc
     1021 atggccgaga tgatcacctc gtccccggcg cgcaggcggc tccggccgta gcattgggcc
     1081 acgaggttga tgccctcggt ggtccctcgg gtgaagatga tctcgtgatc gtgggccgcg
     1141 ttcagcaact gccgcacccg ggtccggccg gattcgtacg cctgggtggc gacctggctc
     1201 aggaagtgga ccccccgatg aatgttcgag ttctcctccg tgtagtaccg ggtcagggcc
     1261 tcgatgacga cccggggttt ctgcgtggtg gcggcattat ccaggtacac caatggcttg
     1321 ccatggacct tcgtctgcag gacgggaaaa tcctcccgga tcttctcgac gccaaacccc
     1381 atcaggcagt cctggcacac ctcttctgga acgcggcggc ccgtgctctt catcgaacgt
     1441 cctccaaggg tctagcctgg ggcaaccact gggtgaccaa tccctccagg tacttccgca
     1501 gcggctcgat cttcatgcgc tgcaggctct ccccggcaaa ggcgaagagc agcagggcct
     1561 tggccgacgc ctcggcgatg ccgcgggagc ggaggtagaa gatggcctcg tcatcgattt
     1621 gaccgatggt cgccccatgg gtgcacttca cgtcgtcggc atagatctcc agttgcggct
     1681 tggtgtcgat ctgtgcgtct tcggagagca gcaggttcat gttggtctgc ttggcgtcgg
     1741 tcttctgcgc atccttgtgc acgatgatcc ggccgtgaaa cacgcctcgc gcctgaccgt
     1801 ccaggatgcc cttgtagaat tggcggctgg agccgtgtgg gctggcgtgc tccacctgca
     1861 tgtagttgtc catgtgctgg cggccggtgg ggatgaacag gccgttgatc atgcactcgc
     1921 agccttcgcc tgccagcacc gggtggatgt tattccggac cagggcgcct cccagcagca
     1981 ccgagtggga ggtcacgttg ctgctccggc cctgctccac tcgcagcgtc gagacattga
     2041 aggaccgggt gttctcccgc tcgatcaaat agtggctcac cacgctgttc tgcccgacga
     2101 ccagctcggt gatcgcgttc gagaaggcca ccccgccgcc gagggagacg tagtcctcca
     2161 gcacggtcgc ctggctgtcc tctcccgaca cgatgagatt gcgcgggtga gtgacggtcg
     2221 gaacggtcag gtctgtcgag acgtaaagca agtggaccgg ctcctccagc accatgctac
     2281 gggggatcga gatgaacgcg ccgtcctcca tgcaagcggt gttcaaggcc aaaaaggggt
     2341 cccggtgaat gtcggcgtag cgggccaggt ggggctccag ggcgtgacga tcctccgcca
     2401 tggcccggcc gaggctgctc accctcagtc ccttcggcag cgcgcccatg gaggacagtt
     2461 tcggcacgaa ccggccgttc acgaagacga gccgcatgca gggcagaccc ggcaccagga
     2521 acgggccgag cgtatccagc gtcaccgccg tctgggcgtc cggagcgggc tggaagtcga
     2581 tttgcgcgat gggcgccacg ctggtgaacc gccaatcctc gtctcggacc gtcgggaatc
     2641 ccacctcgga gaagcggagg actgcggcct tcctgactgg ggtgaaccac gactggctct
     2701 gcggaatgga gcgtaccagt tcctcgaagt gcgacccgta tgtgtgcgtc tcctgcgcct
     2761 gtgtgagtgt catttccgtc gcgcccccgt ctatagagtt gccggagcgg tgagacgcgc
     2821 ctcgttctcc acccaggcgt accccttctc ttccagctcc agggccagct ccttgccgcc
     2881 ggacttgacg atccggccgt ccaccaggac gtgcacgaag tccggcacga tgtagttgag
     2941 cagtcgctgg tagtgggtca cgaccaggat ggcccgctcg ggggcccgca acgcgttgac
     3001 gccgttggcc acgatccgca gggcgtcgat gtccaggccg gaatcggtct cgtccaggat
     3061 ggcgaactgg ggctgcagca cggccatctg gaagatctca ttgcgcttct tctcgcctcc
     3121 cgagaagccc tcgtttaccg accggttgag caatcggtcg tccatctcca ggagcttcat
     3181 cttgtccttg accagggcca gaaagtccat ggcgtcgaat tcgtcctcgc cgcggtactt
     3241 gcggacggcg ttgacggccg ccttgaggaa gtacacgttg ttgacgccgg ggatctccac
     3301 gggatactgg aaggccatga agactccttc gcaggcacgc tcttccggcg ccatctccag
     3361 cagattcttg ccatggtaga ggacgtcccc ggcggtgacc tcgtacgtct cccgtccggc
     3421 caggacctgc gccagcgtgc tcttgcccga cccgttgggt cccatgatgg agtggacctc
     3481 gccggcgttg atcctgaggt tcacgccgcg caggatctcg cgattcccga ccttggcgtg
     3541 gaggttcttg atttcaagca tcgtgtatcc ttccctggtt gagttggtga atgattggtg
     3601 acccgtgagg tcccgtcatg aagacgttct cgtgacgttc cgtctgagga atcagcccac
     3661 gctgccctcg aggctgacgc tcagcagctt ctgcgcctcg acggcaaact ccatcggcag
     3721 ctccttgaag acctgcttgc aaaagccgcc gacgatgagc gagaccgcgt cctctttgga
     3781 gatcgcccgc tggttgcagt agaagatctg gtcctcgccg atcttggacg tggaggcttc
     3841 gtgctccatt cgcgccgtgg cgttcttcac ctcgatgtag gggaaggtgt gggcgccgca
     3901 cttgtcgccg atcaagagcg aatcgcactg cgtgtagttc cgcgccccgg cggccccctt
     3961 caaaatcttg accatccccc ggtaggtgtt ctggccgtga cccgcggaga tgcccttgga
     4021 cacgatggtg ctcttcgtgt tcttgccgat gtgaatcatc ttggtcccgg tgtcgacctg
     4081 ctggtaattg ttggtcaggg ctaccgagta gaactcgccc accgagttgt cgccctgcag
     4141 gatgcagctc gggtacttcc aggtgatgga agagcctgtc tccacctgtg tccaggagat
     4201 gtgggagttc cgccccaggc acttgccgcg cttggtgacg aagttgtaga tgcccccctt
     4261 cccctccttg tccccggggt accagttctg aatggtcgag tacttgatcg tcgcgtcgtc
     4321 gtgggcgacc agctccacca cggccgcgtg caactgattg gtgtcccgca tgggggcggt
     4381 gcagccctcc agatagctca cgtacgcgcc ctcgtcggca atgatcaggg tgcgctcgaa
     4441 ctgcccggtg tttagggcat tgatccggaa gtacgtggac aactccatgg ggcaacgcac
     4501 gcccttgggg atgtagcaga acgatccatc gctgaagacg gcggagttca gcgcagcgta
     4561 gaagttgtcg gtgtagggca ccaccgatcc cagatacttc tggaccaggt ccgggtggtt
     4621 ctgcaccgcc tcggaaaagg agccgaagat gatccccagc tccgccagct tcttctggaa
     4681 cgtggtggcg accgagacgc tgtcgaagac cgcgtccacc gccacccccg cgagctgctt
     4741 ctgctcctgc aaggggatcc ccagcttctc gaaggtccgc agcagctccg gagccacctc
     4801 atccaggctc ttcaggggct tcttggactt gggtgccgaa taatagatga tgtcctggta
     4861 gtcgatcggc ggaaacttca cgttggccca gcgtggctcg ccctcggacc ggaccatctt
     4921 ctcccagtgc cggtaggccc ggagccgcca ctgcagcatg aagtcgggct cttttttctt
     4981 ggcggagatc aggcggatga tgtcctcgct caagccgcgg ggcgctgcgt ccgcgtcgat
     5041 gtccgtcacg aacccgtact tgtattcctg gtttgccagg gcctcgatcg cgtttgtcga
     5101 agtgctcatt atctctccct tgtgggctgc attctcaaga gcttctcttc agtcgagcag
     5161 ggtattatag catcctaagg gctcatcgca tcaccacaac cggctcctgg ttctgagcca
     5221 gcccagcggc gttcaggatg ctgatttgtg ctgccagcct cgccgcgatc tcgtggaatg
     5281 cagccgccac gggaacctcg gtgccctcca gggccaccgg cgttccgccg tcactcttga
     5341 cacggatgcc agcgtccagg gggatttccc cgaggaaggg tatctcggac tgcctgctgg
     5401 cctcccgggc gcccccatgc ccgaagatgt cgtcccgttg cccgcagtgc gagcaggtgt
     5461 ggtagctcat gttctccacg atccctagga tcggcacctt catcttgttg aacatgagga
     5521 ttgccttgga agccacctgg agagccacgt cctggggcgt ggagacgatc acggcccctg
     5581 taagcgggac ggattgacac aagctcagct ggacgtcgcc cgtaccgggc ggcaggtcca
     5641 tcaccagata gtccaactcc ccccagtcga cgcgacccag gaattcctga atcatcttgt
     5701 gcagcatggg gccccgccag atcaccgcct cactcttcgg aaggaagaag cccatggaca
     5761 tgatcttcac gccgtgcagg atgggcggga tgatcttgcc gttggtcccg tgctccagct
     5821 cgccattggc tcccattagg gtcgggatgc atggaccgta cacgtcggta tccatgaggc
     5881 cgacccgggc gcccgatcgc atcaaggcta ccgccaggtt cgccgccacg gtggatttgc
     5941 caacccctcc cttgccgctg gccacggcaa tcgtgttccg aacgcccggg agatactcgg
     6001 aatgcggcgc ccgggaggtc gtcacccgcg aggtcatcgt gacctcgacc gcctggacgc
     6061 cggggagggc cgatacgacc cggcgcgccc cctcctccat ctgatgtttg accggacagg
     6121 cgggggtcgt cagctcgatc atgaagctca cctttccgcc atcaatcttg agacccttga
     6181 cgaatccaag ggacacgatg tcttgatgca gatccggatc cttgatcgtc gccagcgcgg
     6241 ccagaatcgc ctgttccgtg accatgttgt ctcgcttctc ctctcgtcta gttgtctagg
     6301 ttttcctcgt gcgtcgacag acgctgccgg cattagctac gaccggtgcc aatcgccgcc
     6361 ttcctttcaa cctgcgcgag cgtcgccagc agggaggccg tctcattgcc ggggctcccg
     6421 cccagcaacg agaagggaac ccctttctgg gcgagggccc gtgcatcggg cactggcttc
     6481 gtggacaccg ccaagatcgg cagatcgggc gccagctccc tgatggcgga gatcaagagg
     6541 agcccgccga gtccaggcgt ctccaagtcc agcactagga ggtcggaatg caggactccc
     6601 acgactccca gcacctcgag cccggattcg cagacggtgt gacgatgctc acgatgatcg
     6661 aacgcccgcg aggccgcatc cacgaccgcc ggattgcccg aacatacgat gacgtgcata
     6721 ctcttcaacc catccacctc agaacgagga aactgtctcc tagcctttga ggtttcttgt
     6781 agggcaagcc tcgtaccaca ctagacaccc ctagaatatt tctcaggtag ttttcataac
     6841 tcattgtaaa tacaacg
//
LOCUS       DBNGZC010000571         4489 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1711428, whole genome shotgun sequence.
ACCESSION   DBNGZC010000571 DBNGZC010000000
VERSION     DBNGZC010000571.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1711428"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1464)
                     /locus_tag="AC16G0_09965"
     CDS             complement(82..1464)
                     /locus_tag="AC16G0_09965"
                     /EC_number="5.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008477365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR5099976.1"
                     /translation="MMQKQGKDQEGHGRIWEAFQRANPRSAELFARAGQVIPGGITHD
                     IRQLKPFPIYVERAQGTRKWTVDGQELVDYWMGHGALFLGHGHPTILRAVHEQLDRGT
                     HYGACHELEVRWAEQVQRLVPSAERVRFTSSGTEATQLALRLARAYTGKPKVVKFEGH
                     FHGWHDYALIGVKPPYEAPMSAGIPGDTLGQVILCPPNDLAAFESRLDSHQDVAAVIL
                     EPGGGSSGTIPTDAAYLRGLREVTRRREVVLIFDEVITGFRQAPGGVQEVVGVTPDMT
                     TLAKILAGGLPGGAVVGKAAILERLAFKDDPNWNRKARVAQAGTYNANPLAAAAGIAM
                     LTLIADGEAHRRANRTAEVLRQELGGVWRRLGIPGCVYGEASTINYSLEAALGSAPAP
                     GARDHGRLQVLKNPDVYHALRCALILNGADICPLHGWVSAAHTDEDVQRTVQAFEKAL
                     LLMREDGLLP"
     gene            complement(1511..1789)
                     /locus_tag="AC16G0_09970"
     CDS             complement(1511..1789)
                     /locus_tag="AC16G0_09970"
                     /inference="COORDINATES: protein motif:HMM:NF017574.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR5099977.1"
                     /translation="MDDRPLRLRLYSRPGCHLCDNLRVLCERLSGEFPLQLTEVNIET
                     DPGLLAQYDQKVPVLFINGRKAVKYRTTEAALRRLLQRRLLVSRLFGT"
     gene            complement(2054..2980)
                     /locus_tag="AC16G0_09975"
     CDS             complement(2054..2980)
                     /locus_tag="AC16G0_09975"
                     /inference="COORDINATES: protein motif:HMM:NF040052.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6306 domain-containing protein"
                     /protein_id="HIR5099978.1"
                     /translation="MPKSPRKKIEPRKARGRQPVHPPLAPPPSREGHRPTPISDEEIV
                     GRLNELLEAERAGVEAAAALRQVSAGSMTAAELDKFADDEASACAGLHRCILRYGGQP
                     SDRTGDFGRRVAALKDEGERLNLMTRGQAWVVKRLEVLLGMPLDAETRIFLAEMRAEH
                     LENIEACNRRAEEVSAPPSPPYRELPFARLREAHDRLYYGPWRTPTATLRYYQRGYFQ
                     LVRYLDMLAEEVQRTRSVEARRALARARAAHAKADPEADRGEVIRSLDNSLSYAHSAL
                     NALLRQYRAPSHDPRDFESFYDVIGVPFEDLV"
     gene            complement(3223..3915)
                     /locus_tag="AC16G0_09980"
     CDS             complement(3223..3915)
                     /locus_tag="AC16G0_09980"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR5099979.1"
                     /translation="MKNIQDCATCRLREVAALCSGGKSSLAAFMRGRSRHRYLAKQTL
                     YHENTPALGVYGVCSGQIKVSRSGGRGKEQILRIVDPGGILGEESLLEGGRFAGTAQA
                     LGNSEVAFVGRREMLQLLRAPDAMTEKFVLHLCHVLQATQMDLARIALADARTRLAGL
                     LLDLGRRYGESEGKGVRFMLNVSRRELGAMVGLTPETTMRLMSDFRRQGLVRADGRML
                     SLPNPDRLEGSA"
     gene            complement(4167..4340)
                     /locus_tag="AC16G0_09985"
     CDS             complement(4167..4340)
                     /locus_tag="AC16G0_09985"
                     /inference="COORDINATES: protein motif:HMM:NF013286.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HIR5099980.1"
                     /translation="MNESQVNPGEAERRFEQQIEAVLEKIRPALQADGGDIELLAVEG
                     RNARVRLVGACGG"
BASE COUNT          772 a         1561 c         1354 g          802 t
ORIGIN      
        1 ctcgcgtggt tgtccggctt cgctctcagg gcagtgaatg gcgccgcgcc gccgtcggcg
       61 agcgcggcgc ggatacgccg gtcacggcaa aaggccgtct tcgcgcatca agagcagcgc
      121 cttctcgaag gcctggacgg tgcgctgtac gtcctcgtcg gtgtgggccg ccgaaaccca
      181 cccgtgcagc gggcagatat ccgcgccgtt caggatcaga gcgcagcgca gcgcgtggta
      241 gacatccgga ttcttgagga cttgcaggcg gccatggtcg cgggctcccg gcgccggggc
      301 cgaccccagc gccgcctcca gggaatagtt gatggtggag gcctcgccat agacgcaacc
      361 agggatgccg aggcgtcgcc acacccctcc cagctcttgc cgcaagacct cggccgtccg
      421 attggcgcga cgatgcgcct ctccgtctgc gatgagggtc agcatggcga tgccggccgc
      481 ggcggccagc ggattggcgt tataggtacc tgcctgggcg acccgcgcct tccgattcca
      541 gttgggatcg tccttgaagg ccagccgctc caggatggcc gccttcccca cgaccgcacc
      601 gccgggcaac ccgcccgcca ggatcttggc cagcgtcgtc atgtccggag tgaccccgac
      661 cacctcttgg accccgccgg gcgcctggcg gaacccggtg atgacttcat caaagatcag
      721 cacgacctca cggcgacgcg tgacctcgcg caggcctcgc agatatgcgg catccgtcgg
      781 gatcgtgccg ctgctgccgc cacccggctc caggatcacg gcagccacgt cctggtggga
      841 atccaaacgc gactcgaatg cagccaggtc gttgggcggg cagaggatga cctggcccag
      901 cgtgtcgccg gggatccccg ccgacatcgg ggcctcgtag gggggcttca ccccgatcag
      961 ggcgtagtcg tgccagccgt ggaagtgccc ctcgaacttc acgaccttgg gcttgccggt
     1021 gtaggcgcga gccagacgca gggcgagctg cgtggcctcg gttcccgagc tggtgaaacg
     1081 gacacgctcc gccgacggca cgagccgctg cacctgctcg gcccagcgaa cctccaactc
     1141 gtgacacgcc ccataatggg tgccgcggtc gagctgttcg tgaaccgccc gcaggatcgt
     1201 cgggtgaccg tggccaagga acagggcccc atgccccatc caatagtcga ccagctcttg
     1261 gccgtccact gtccactttc gcgttccctg ggctcgctct acgtatatcg ggaacggctt
     1321 caattggcgg atatcgtggg tgatacctcc ggggatcacc tgcccggccc gagcgaacag
     1381 ttccgccgag cgcgggttcg cccgctggaa tgcctcccag atccgcccgt gcccttcctg
     1441 atcttttccc tgtttctgca tcatgatcgc tctcacctct ctgctgcgtt cgaagcggcg
     1501 cgccgtagtc tcacgtgcca aacagtcggc tcacaagcag gcgacgctgg agcagccgac
     1561 gaagggccgc ctcggtcgtt cggtacttga ccgcctttcg cccgttgatg aagagcactg
     1621 gaaccttctg atcgtactgg gcgagcaacc ctggatcggt ctcgatgttc acctcggtga
     1681 gctggagagg gaactcgccg gacaaccgct cgcagagtac tcggaggttg tcacacagat
     1741 ggcatccggg tcggctatag agtcttaggc gaagcggccg atcatccata tatcttgtgg
     1801 cgcagaaacg ttaccacgcc cctcgggggc tgtcaacgcg cccgggcaca ttactatcct
     1861 ctccagccgg gagggaacga gaacgaacga tcgaaagaat ctaggatcag ccggatggct
     1921 accagggaac aggagcttgc ggccaatagg gtatcatgct ggaaccacag tcgcgtcaga
     1981 accttatctc gaatagaaga ctttcctcat aactatctga tttaatggaa tatcgtccat
     2041 cgtggttcca gcctcatacc agatcttcga atggcacacc gatgacatca taaaaggact
     2101 caaagtcacg gggatcgtgg ctgggcgctc gatactgccg taggagggca ttcagggcgc
     2161 tgtgcgcgta cgacaggcta ttgtcgaggc ttcgaatcac ctccccccga tccgcttccg
     2221 ggtcggcctt ggcatgagcg gcgcgcgccc gggccagggc gcgcctggct tcaaccgacc
     2281 gggtgcgctg gacctcctcg gccaacatgt ccaggtaacg gacgagttga aaatatcctc
     2341 gctggtaata ccggagggtc gccgtcggcg tcctccaggg gccatagtag agccgatcgt
     2401 gagcttcgcg aagccgcgcg aagggcagtt cacgataggg cgggctgggg ggtgcggaca
     2461 cttcctccgc gcggcggttg caggcctcga tgttctccag gtgttcagcc cgcatctcgg
     2521 caaggaagat ccgcgtctcc gcgtccagtg gcattcccag gagcacctcc aggcgcttga
     2581 ccacccaggc ctggccccgg gtcatcagat tcaagcgctc tccctcatcc ttcagggcag
     2641 ccaccctgcg gccaaaatcc ccggttcgat ccgacggctg cccgccgtaa cgcaggatac
     2701 accggtgcag cccggcgcac gcagaagcct cgtcgtccgc gaacttgtcc agctcggcgg
     2761 ccgtcatact cccggcggaa acctgccgga gcgctgctgc ggcctcgacg ccggcccgct
     2821 cggcctccag gagttcgttc agccgaccga cgatctcttc gtccgagatc ggggtcggcc
     2881 gatggccctc ccggctcggt ggcggggcga gggggggatg aacaggttgc cgccctcgcg
     2941 ctttccgggg ctcgatcttt ttccgcggag attttggcat ggccactcgg cgcgtcgcgt
     3001 ccagaggcag gaaacttgcg attgatccag aggaggatgt tctggaacgg cggcaagctg
     3061 tggtcggtcg cagcctacag agtcggccgg cccctagcca gctgcccccc atcttccagg
     3121 cctagcgtga gcctggtccg cggacctccg gaacgcccac cgaggaatac taggatcttt
     3181 gctcgcgctc tgcaatggtc tcgggagagc atcgaagcac tgctacgccg acccttccaa
     3241 tctgtccgga ttgggcaggc tcagcatgcg cccatccgcc cggacgagtc cttgccttcg
     3301 gaagtcggac atgagacgca tggtcgtctc cggggtaagg cccaccatcg cccccagttc
     3361 cctgcggctg acattcagca taaatcgcac accctttccc tcgctctccc catagcggcg
     3421 gcccaagtcc agcaggagcc cggccagacg cgttcgcgcg tctgccaggg cgatgcgggc
     3481 gaggtccatc tgggttgcct ggagcacgtg acacaggtga agcacgaatt tctctgtcat
     3541 ggcatctggg gcacggagga gctggagcat ttccctgcgg ccgacgaagg caacctcgct
     3601 attccccagc gcctgcgctg tgccggcaaa gcgaccaccc tccagcaggc tctcctcacc
     3661 caggatccca ccagggtcca cgatgcggag gatctgctct tttccgcgtc ccccactccg
     3721 gctcaccttg atctggccgc tgcagacccc gtagacaccc agggccgggg tgttctcatg
     3781 gtacagggtc tgcttcgcca ggtaccgatg ccgggagcgc ccccgcatga acgccgccag
     3841 cgaactcttg ccaccgctac acaaggccgc gacctcgcgc aggcggcagg tcgcacagtc
     3901 ctgaatgttt ttcattgccg ccatcctctg ggctcgcgtt tgagctcagt ctcacccgca
     3961 ccaccgcccc cgtccagcct ggcccggcca ccgcggaagc agcctgcacc acccggcggc
     4021 cctcccactc ctcggtgcgt ttccagtcga tcgagtcgct agtccaatcc gccctcagac
     4081 gtgtcgctga tcacctcacc aaagccgggc acctcgtgct gcagaaatcg ctgcactccc
     4141 atctggagtg tgatgtgggc gctgggtcac ccgccgcacg ccccgaccag ccgcaccctg
     4201 gcattgcgtc cctccaccgc cagaagctcg atgtcccctc cgtcggcctg gagggccggc
     4261 cggatcttct ccagcaccgc ttcgatctgc tgctcgaatc tccgttcggc ctcgcccgga
     4321 ttcacttgcg actcgttcat ggtcccaccc cttccagaaa gaagacctcc agccaccatc
     4381 cgagaatgaa accgaccttg cccgcctcac cagtgtctcc atgacatgcg tattcgcacc
     4441 gcccccagga gcccgctgaa atcgcccgaa cgactcatcc gtccgccga
//
LOCUS       DBNGZC010000572         7918 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1223360, whole genome shotgun sequence.
ACCESSION   DBNGZC010000572 DBNGZC010000000
VERSION     DBNGZC010000572.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7918)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7918
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1223360"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="AC16G0_09990"
     CDS             complement(<1..617)
                     /locus_tag="AC16G0_09990"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5099981.1"
                     /translation="MANEFRRTLMRLLGRMPGRCALRPGVVERVALAGFVREKVNYAV
                     APGERVSAYLFLPDGDAPRPAVLCIHQHNREFHLGKSEPAGLSGNPEQFFALELAKRG
                     YVTLCPDAIGFEERQHPILRGADYERFEATRLLTLGSSLQAKMLSDLMRALDYLTTRK
                     EVDRRRLGCLGHSLGGQETLFLSATDRRIAAAVSSCGFSSYQAIF"
     gene            complement(626..2275)
                     /locus_tag="AC16G0_09995"
     CDS             complement(626..2275)
                     /locus_tag="AC16G0_09995"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5099982.1"
                     /translation="MAWRRRDFWWSITALAFLAVLAFLVYPLATVLFTSFMGEEQRFV
                     LAENYHRFFAHRYYYGSIINSLYLSSLATLGALALGTSLAFAVARYPLPGKTFLRTAA
                     NLALLSPPFIGAYSWVVLFGRMGLVNRALSTFGWSLPTIYGWRGILLVFIVQYFPFVF
                     LLVSAALRSVDQSIEDAALSLGSPPRRALTTAILPILAPSLTAGALLVFMATMADFGT
                     PMIIGEGLRTLPTLMYGEFINELGGNPAMASTLGSLLILVNTAALMAQRYYAFRKAYN
                     VVCIQPLQVRRLQPGRRLALAVYAYAVVAVALIPSVTIIISSFMPTRGPVMYAGLSLQ
                     SYRTIFYLIPRAIANTFGLTAAAVVLDVLLGVLVAYLLARRPSRTHALLDGLIMLAYA
                     IPGTVVGVGLIVVYNRPPVVLTGTWVILLVSYFIRHLPYPVRSCTAMLQQIDIRVEEA
                     SVSLGVPPFRTFLKVTIPLMFPAIISGAALTWMTTIGELSSSILLYSGPWATMSVAIF
                     TEVFSNHFGTASALASILIAGAFVPLFLTYRFLGERAALGV"
     gene            complement(2277..3371)
                     /locus_tag="AC16G0_10000"
     CDS             complement(2277..3371)
                     /locus_tag="AC16G0_10000"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5099983.1"
                     /translation="MTRVRVQGIVKRFGETPAIDGVSLDVRQGELFTLVGPSGCGKTT
                     LLRIMAGLLEQDVGSVLFNDDRMDEVPPYLRNIGVVFQNYAIFPHLTVGENIAYGLKA
                     RRVASERIAAKVEEASRLVQLEGLLDRTPAQLSGGQQQRVVLARALVIEPRLLLMDEP
                     LSNLDAKLRVQIRSLIRAVQRKLQITTIYVTHDQEEALAISDTIAVMDRGRVLQVGKP
                     WELYLRPDNRFVADFIGTMNVLPAQVEGPGQSPSDLAVMTEFGERWQLRAAGQPGEAI
                     SIGVRPESLQLEPGPNASDAWNALKGTVSEVAYLGAVVRYHVQVTADRRVVADIHNPD
                     FTAICAVGDRLTLWFAGSRAILLPAEVGGD"
     gene            complement(3463..4479)
                     /locus_tag="AC16G0_10005"
     CDS             complement(3463..4479)
                     /locus_tag="AC16G0_10005"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5099984.1"
                     /translation="MRQARGWLLGVMAISCMLAVVVGTIWAQGKPEDSVVVYSAADAD
                     MVNAVAAAFEKKYPGIKVSTVVAGTGEIIKRIEAEKERPLGDVGWSFGPEAIGDKKGL
                     FEPYLSNEASAFFPGEVPADKVWTPFTTMPYVIMYNKKLVSEAEKPKAWKDVLEAKWK
                     GKVAYADASKSGSSYTLLVTWLTIYGKNDTGWKFVEDLLRQCKVLPKSSMTYQGVANG
                     EYPIGLTFEQAAFEYLKGGAPIGLIYPTEGTAITLDGSAIVKNAPHPNAAKLFLDFTV
                     SKDMQQLMVEKFGRRSVRKDVGSPAGLPMLDKIKAINYDLDYAANQRGQLLKRFQNTL
                     IKTQ"
     gene            complement(4635..5153)
                     /locus_tag="AC16G0_10010"
     CDS             complement(4635..5153)
                     /locus_tag="AC16G0_10010"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4956..4958),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HIR5099985.1"
                     /translation="MEQPGRHPHGAIVRQGEPHMPEIKIGDPAPDFRLPAPGNKEIGL
                     ADFRGKRHVVLAFYPFDWSPGUTKEMPALEARLADFQAAGAAILGISIDSVYSHDAWA
                     DHHKLTFPLLSDIHRKVCQAYGVHNAERNCARRSVFIVDKGGMVSFKEEYGPGQLPDP
                     ETLLAALKVLGT"
     gene            complement(5153..5896)
                     /locus_tag="AC16G0_10015"
     CDS             complement(5153..5896)
                     /locus_tag="AC16G0_10015"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR5099986.1"
                     /translation="MKIYQAVLLDMFDTLVNFNNQRLPLVQINGQEVRSTSPYVYEVL
                     KTVCAEVSLAEFYQAFVGAFRAAEEIRNRDQREVTAHERFRMLFQRLGISDGSEVARF
                     IDIGIAEHTRQLACAMEFPESHRTVLEALRPRYRLAVVSNFDHGPTVEMALESHGIRQ
                     SFEVVVVSADVGWRKPRPEIFAETLRRLGVRPDEVIFVGDTPEVDVVGAQAAGMDVIW
                     IDHGVKPLPPGTPAPTYTVSAFTDIAFLL"
     gene            6278..6433
                     /locus_tag="AC16G0_10020"
     CDS             6278..6433
                     /locus_tag="AC16G0_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016514 - SWI/SNF complex [Evidence IEA];
                     GO_function: GO:0061629 - RNA polymerase II-specific
                     DNA-binding transcription factor binding [Evidence IEA];
                     GO_process: GO:0006338 - chromatin remodeling [Evidence
                     IEA];
                     GO_process: GO:2000819 - regulation of nucleotide-excision
                     repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB-MDM2 domain-containing protein"
                     /protein_id="HIR5099987.1"
                     /translation="MATKKAAFGGYTINFKGCTETVEAVFGNKPVSPSDMTKKLWAYV
                     KKNKLAK"
     gene            6711..6899
                     /locus_tag="AC16G0_10025"
     CDS             6711..6899
                     /locus_tag="AC16G0_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099988.1"
                     /translation="MATSGQFIAITLHRIPRKEVCGVVILARQEDESWAGKCSKCGED
                     FRVERDPKFEAQVRAMRN"
     gene            7156..7638
                     /locus_tag="AC16G0_10030"
     CDS             7156..7638
                     /locus_tag="AC16G0_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099989.1"
                     /translation="MAQRWRGKTLESLEGKLAAIIRREVQKVLGVLKREISKREGELA
                     TLKAEYKKGLDILRRRQKTGPARRRRRGRQANWKQVFSSLPTHFTLETLLRHPVAGKR
                     SKPHLYAIISRWKKDKLLTPDPAGGYRKIGAQVKKKHVPRAKPAHAPKPATRAEAPGA
                     "
     gene            complement(7684..>7918)
                     /locus_tag="AC16G0_10035"
     CDS             complement(7684..>7918)
                     /locus_tag="AC16G0_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5099990.1"
                     /translation="KPMRPPHYARRSDTMRIWISLAYLSLAASLVSLGIANGSWLLNS
                     PAAGLSPRAYALGAGIALLYAIAFLMFDQAQRS"
BASE COUNT         1552 a         2480 c         2524 g         1362 t
ORIGIN      
        1 tcgaagatcg cctggtagct gctgaatccg cagctcgaca cggcggccgc gatgcgtcga
       61 tccgtcgcgc tgaggaagag agtctcctgc ccccccagtg aatgccccag gcatcccagc
      121 ctgcgccggt ccacctcctt ccgggtcgtc agataatcca gcgcccgcat gaggtccgag
      181 agcatcttgg cctgcaggga cgaccccagc gtaaggagcc gggtcgcctc gaagcgctcg
      241 tagtccgccc cccgcaggat cggatgctgc cgttcctcga agccgatggc gtcggggcag
      301 agcgtgacgt agccccgttt ggccagttcc agggcgaaaa actgctcggg gttgccggag
      361 aggcccgcgg gctcactctt ccccaggtgg aattcccggt tgtgctggtg gatgcacagg
      421 accgccggac ggggggcgtc tccgtccggg aggaacaggt aggcgctcac ccgctccccg
      481 ggagccaccg cgtagttcac cttctcccgg acgaatcctg caagcgccac ccgctccacc
      541 acccctggcc ggagcgcaca gcgacccggc atccgcccta gcagccgcat cagcgtccgt
      601 cgaaattcgt tggccatctt tcccctcaca caccgagcgc ggcgcgctcg ccaaggaagc
      661 ggtaagtgag gaacaacggc acaaaggctc ccgcaatgag aatcgaggcc agggccgagg
      721 ccgtcccgaa gtggttgctg aagacctcgg tgaagatggc gaccgacatc gtggcccacg
      781 ggccggagta gagcaggatc gaggagctga gctcgccgat ggtggtcatc caggtcaagg
      841 ccgccccgga gatgatcgca gggaacatga gggggatcgt caccttcagg aacgtgcgga
      901 agggcggcac ccccaggctc accgaggcct cctcaacccg gatgtcgatc tgctgaagca
      961 tggcggtaca ggagcgaacc gggtagggca ggtggcggat gaagtaggag accagaagga
     1021 tcacccaggt gcccgtgagc accacgggcg ggcggttata gaccacgatc agccccacgc
     1081 ccacgacggt accggggatg gcataggcca gcatgatcag cccatccagc agggcgtggg
     1141 tgcgggacgg ccgccgggcc aagaggtatg ccaccagcac ccccagcagg acatccagga
     1201 ccaccgccgc cgccgtgagg ccgaaggtgt tggcaatggc ccgggggatg agatagaaga
     1261 tggtccggta gctctgcagg ctgaggcccg catacatcac gggaccccgc gtcggcatga
     1321 aggaggagat gatgatggtg accgatggga tgagagccac ggctaccacc gcataggcat
     1381 acacagccaa ggcgagccgc cggcccggct gcaggcgccg cacctggagc ggctggatgc
     1441 agaccacgtt ataggctttc cggaaggcat agtaacgctg ggccatgagg gccgcggtgt
     1501 tcaccaggat gaggagactc cccagcgtgc tggccatggc cgggttgccc cccagctcgt
     1561 tgatgaactc tccgtacatc agcgtgggca gcgtccgcag cccctcgccg atgatcatgg
     1621 gggtgccgaa gtcggccatg gtggccatga agacgaggag cgcccccgcc gtgagcgacg
     1681 gggccaggat gggcaggatg gccgtggtga gcgcccggcg cggcggcgag cccaggctga
     1741 gggcggcgtc ctcgatggat tgatccacgg agcgaagcgc cgctgacacc agcaggaaga
     1801 cgaaggggaa gtactggacg atgaagacca gcaggatgcc gcgccagccg tagatggtgg
     1861 gcagactcca tccgaaggtc gacagcgccc ggttgaccag ccccatccgc ccgaacagga
     1921 cgacccacga gtaggccccg atgaacggag gcgacaggag ggccaggttg gccgccgtgc
     1981 ggagaaaggt cttgccgggc aaaggataac gcgcaaccgc gaaggccagg gatgtcccga
     2041 gggcgagtgc ccccagggtg gccaggctgc tcaagtacag gctgttgatg atgcttccgt
     2101 agtaatagcg gtgggcgaag aagcggtggt agttctccgc caggacgaag cgctgctcct
     2161 cgcccatgaa gctcgtgaac agcacggtgg ccagcgggta caccagaaac gccaggacgg
     2221 cgagaaaggc cagggccgtg atggaccacc agaagtcgcg gcggcgccag gccatatcag
     2281 tctccgccca cctcggcggg cagcaggatg gcccggctcc cggcaaacca gagcgtgagg
     2341 cgatccccca ccgcgcagat cgcagtgaag tccggattgt ggatgtcggc gacgacgcgg
     2401 cgatcggctg tcacctgcac gtgataccgc accaccgccc cgaggtacgc cacctcgctg
     2461 accgtgcctt tcaaggcgtt ccaggcgtca gaggcgttgg ggcccggctc gagctggagc
     2521 gactcgggcc ggacaccgat gcttatcgcc tccccgggtt gccccgcggc ccgcagttgc
     2581 caccgctcgc cgaactccgt catcaccgcc agatcgctgg gcgactgccc tggaccctcc
     2641 acttgggcgg gcaggacgtt catcgtcccg atgaagtcgg cgacgaatcg gttgtcgggc
     2701 cggaggtaga gctcccaggg tttgccgacc tgcaggacgc gcccccgatc catcaccgcg
     2761 atggtgtcgg agatggcgag ggcctcctct tggtcgtggg tcacgtagat ggtcgtgatc
     2821 tggagcttcc gttgcaccgc tcgtatgagc gagcggatct gcactcgaag cttcgcgtcc
     2881 aagttggaga ggggctcgtc catcaagaga aggcgcggct cgatgacgag cgcccgggcc
     2941 aggacgaccc gctgctgttg cccgccggag agctgggccg gcgtccggtc cagaagcccc
     3001 tccagttgaa cgaggcgcga cgcctcctcg accttggcag cgatgcgctc ggaggcgacg
     3061 cggcgagctt tcaggccata ggcgatgttc tcgcccacgg tgagatgcgg gaagatggcg
     3121 tagttctgaa acaccacgcc gatgttgcgg aggtacggcg ggacctcgtc catccgatcg
     3181 tcattgaaga ggacactccc tacatcctgc tccaagagac cggccatgat gcggagcagc
     3241 gtggtcttgc cgcagccgct cgggccgacc agggtgaaga gctctccctg ccggacgtcc
     3301 aagctgacgc cgtcaatcgc cggcgtttcg ccaaatcgct tgacgatgcc ctgcactcga
     3361 acgcgagtca tggacctccg tctctcgggt gaataaacgg aagcgaggcg ggagattcgc
     3421 ctcgcttcag tctggggcgc atggcagcat cacttcccgc gcctactggg tcttgatcaa
     3481 ggtattctga aaccgcttca gaagctgccc gcgctgattg gccgcgtaat ctaggtcgta
     3541 attgatagcc ttgatcttgt ccagcattgg caggccggca ggggatccca cgtccttgcg
     3601 caccgaccgg cggccgaatt tctccaccat caactgctgc atatccttgg agacggtgaa
     3661 atccaggaac agcttggcgg cattcgggtg gggtgcattc ttaacgatgg cggatccgtc
     3721 cagcgtgatg gccgtgccct ctgtggggta gatcaggccg atgggggccc cgcccttcag
     3781 gtactcgaaa gcggcctgct caaaggtgag gccgatggga tactccccat tggccacgcc
     3841 ctggtaggtc atgctggact tgggcaggac cttgcactgg cggaggaggt cctccacgaa
     3901 cttccagccg gtatcgtttt ttccgtagat ggtgagccag gtgacgagca aggtgtagga
     3961 ggaaccggac ttcgaggcgt cggcgtaagc caccttcccc ttccacttgg cctccagcac
     4021 atccttccag gccttcggct tctcagcctc gctcaccagc ttcttgttat acatgatgac
     4081 gtagggcatg gtcgtaaagg gcgtccacac cttgtccgca ggcacttcgc cggggaagaa
     4141 agcgcttgcc tcattggaga ggtagggctc gaataggccc ttcttgtcgc cgatggcctc
     4201 tgggccgaag ctccagccca cgtcccccag cggccgctct ttttccgcct cgatgcgctt
     4261 gatgatctct cccgtccccg ccacaacggt ggaaaccttg atgcccgggt acttcttctc
     4321 gaaggccgcg gccacagcat tcaccatatc cgcgtcggcc gcggaataga ccactacgga
     4381 gtcctctggt ttgccctgcg cccagatggt tccaaccacg acggccagca tgcacgagat
     4441 cgccatgacc cccaagagcc acccacgtgc ttgtctcatg actcgcctcc tttttcgtgg
     4501 cctgccgcgt ttcttttcga tcatgcctac tacaagggcc tggagaaatc aaggagcaaa
     4561 gcacacccca cccagtgcgc cataggctgc gccacacgcg ccgcttgctt tcaaggcccc
     4621 tctcggatgg tttctcacgt gcccagaacc ttcagcgctg ccaggagagt ctcggggtct
     4681 gggagttggc cgggaccata ctcctccttg aaactgacca tcccgccttt gtccacgatg
     4741 aacactgagc gcctggcaca gttccgctca gcgttgtgca cgccgtacgc ctggcaaacc
     4801 ttccggtgga tgtcactcag aagcggaaag gtaagcttgt ggtgatcagc ccaggcgtcg
     4861 tgcgaataga cactgtcaat gctgataccc aggattgccg cccccgccgc ctggaaatca
     4921 gccagccgcg cctccagcgc aggcatttcc ttggttcagc caggtgacca gtcgaacggg
     4981 tagaaagcca ggaccacgtg gcgcttcccc cgaaagtcgg cgaggccgat ctctttgttg
     5041 ccgggggcag gcaaccggaa gtccggagcc ggatcgccaa tcttgatttc tggcatgtgc
     5101 ggttctcctt gtcggactat cgcgccgtgc ggatgcctgc ctggctgttc catcacagca
     5161 agaatgcgat gtccgtgaat gccgacacgg tgtaggtggg tgccggcgtt ccaggcggaa
     5221 gcggcttgac gccgtgatcg atccagatca cgtccatgcc cgccgcttgc gctcccacga
     5281 catccacctc gggtgtgtct cccacgaaga tgacctcgtc cggccggacc cccagccgtc
     5341 gaagcgtctc tgcgaagatc tccggccgag gcttgcgcca accgacatct gcggacacca
     5401 cgaccacctc gaacgattgt cgaataccgt gcgactcgag cgccatctcc accgtgggtc
     5461 cgtgatcgaa attggagacg accgccaggc gatagcgcgg ccgtagcgct tccaggaccg
     5521 tgcgatgaga ctcggggaac tccatggcgc aggccaactg tcgcgtatgc tcggcgatcc
     5581 cgatgtcgat gaagcgtgca acctcggaac cgtcggagat cccgagtctc tggaagagca
     5641 tccggaagcg ttcgtgcgcc gtcacctccc gctggtccct gttccgaatt tcctccgcgg
     5701 ccctgaaggc cccgacgaac gcctgataga attccgccag cgagacctct gcacacacag
     5761 tcttcagtac ctcgtagacg tacggactgg tagaccggac ctcttgtccg ttgatctgaa
     5821 ccagaggaag gcgttgattg ttgaaattca ccagggtgtc gaacatatcg aggagcacgg
     5881 cttggtaaat cttcatggaa tctcgcgacg gacttcgtat gaggtgcctg cgccctctca
     5941 ccccgagcgt ctggtcgacg cgtatatttg ctgaccagaa aactggccac agaatacttt
     6001 cggtcaagag cgccgtcaag gtcggaccgt gaacgctgac caaggggcga agcagtccgg
     6061 atggagccat cccggtcaat cctaatagcc gtcagacgtt tggcttgtac ccaggatatg
     6121 gggacaccag atagtgtcaa ccctatatat tgggcctcat gtgagaaaaa agattgacat
     6181 ggtctggcga gttgatattg tatgtccgcc atcgcccaat agggggcggg catcttgccg
     6241 ggctgtctgt caaagcagac ccggaaagga gaggtgaatg gcgacgaaga aggcggcatt
     6301 cggaggctac accatcaact tcaaagggtg caccgagaca gtggaggccg tcttcggtaa
     6361 caagcctgtc agcccgagcg acatgacaaa gaagctctgg gcgtacgtca agaagaacaa
     6421 gctagcgaag tagtcgcccc tccacccagg tccggaaagc acgcgccatt cagcggatgc
     6481 ccggatgatc gggtagctcc ggagatggtt gtttcacctc tcaacgccac agccctgtca
     6541 tatgacaggg ctgtggcgtt ttaccgtctt ctccttcggg ccccgcatca acgaggggcc
     6601 atccttcctc ttgacctcca ggcctccaag gtggcattct ctcaagggat ccgtgggacc
     6661 tgaaagaatg cccacagact gcccgttgca ccccgacgca ggacatgccg atggccacga
     6721 gtggtcaatt catcgcgatc acgctgcacc gcatcccccg aaaggaggtg tgtggtgtcg
     6781 tcatccttgc ccgacaggag gacgagtcct gggctggaaa gtgcagcaag tgcggcgaag
     6841 acttccgggt ggagcgcgat ccaaagttcg aggcgcaggt tcgtgcgatg cggaattgaa
     6901 ccgagcgtcc tgcgatgggt cttgcttccg tctcggatct gtctctgaag ccgatacccc
     6961 ccattgacaa aggatgatgc ccttccgcgt gaacgtcctg tgtgctagca tccctcggca
     7021 cgttcggcag gtcggagggg ccgcgcctgg tcgcgggatc ggctccccgt ggcggcatca
     7081 tctgtgtcgt catccccgtt ggtctcccct ccccgatggg gcgaggagcc cgaactccaa
     7141 ccacgaggag gagtcatggc gcagaggtgg aggggcaaga ccctggaatc cctggagggg
     7201 aaactggcag ccattatccg gcgggaagtt cagaaggttc tgggagtcct caaacgagag
     7261 atctcgaagc gggagggcga gctcgccacc ctcaaggcgg agtataagaa gggccttgat
     7321 atcctccgca gacggcagaa gactggaccc gcccggagac gacggcgcgg gcggcaggcc
     7381 aactggaagc aggtcttcag ttcccttccg acccacttca ccctggagac gctactacgc
     7441 catccggtgg cgggcaagag atccaagccc cacctgtacg ccatcatttc ccgatggaag
     7501 aaggataaac tgttaacgcc ggatccggca gggggctacc gaaaaatcgg ggcccaagtg
     7561 aagaagaagc acgtcccacg ggcgaagccc gcgcacgcgc cgaaaccggc cactcgggct
     7621 gaggcgcccg gcgcgtaggt gcttccgccg cggggcgagc tgccgcatcg ggatatgggc
     7681 ccctcagctt cgttgggctt ggtcgaacat caggaaggca atcgcgtaca ggagggcgat
     7741 gccggcgccc agcgcgtacg cccgcgggct caggccggcg gcaggtgagt tgagcagcca
     7801 ggagccgttg gcgatcccca ggctgaccag cgacgcggcc aggctcaggt aggccaggct
     7861 aatccagatc cgcatggtat cactcctccg ggcatagtgc gggggtctca tgggcttg
//
LOCUS       DBNGZC010000573         1205 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_635849, whole genome shotgun sequence.
ACCESSION   DBNGZC010000573 DBNGZC010000000
VERSION     DBNGZC010000573.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1205)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1205)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1205
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_635849"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(403..>1205)
                     /locus_tag="AC16G0_10040"
     CDS             complement(403..>1205)
                     /locus_tag="AC16G0_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017639834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR5099991.1"
                     /translation="SVQLPDGSRVPALGQGSATLAQGRHPASEEEDALRLGISLGLTL
                     LDTAELYGNGRAEQMIGRVIAGQRDHVFLVSKVWPSHATATGIRQACAASLGRLGTDY
                     LDLYLLHWPNGVSDLGVVVKTFDRLRAEGSIRRWGVSNFDVADMERLYRVPGGTAYAT
                     NQVRYNLTDRRIERALLPWCEQHRVPIMAYSPLGRGGGLLRNPALAQVADRYQSSPAA
                     VALAWTLRSGHVIAIPESGAPAHVRQNASALSLRLTDQDLDDLNRAFP"
BASE COUNT          210 a          408 c          389 g          198 t
ORIGIN      
        1 tcccccccct cgcacctcgg gcatggttat gggtgatgat ctcgggacca tccgcctccc
       61 ccggcgggac ccggaaaggg tcacagactc gcctttttgc accccagtgt caagggccag
      121 ttggccctct gttctccggc caggaagctg agtagtcgaa ccgaatgcgc gtattatggg
      181 acgcccccca tcacgggttt cgtgcggatc ggcgagagtc ttcgagaact caaacgcggg
      241 ttggaatcac cctggggaca ccactcatat atacgttgaa gcgccttgtg attttaacct
      301 gtcggtgagc gcccgtgcac cccgacacga tgtccttgct ccccaaaaag ctctgccgcc
      361 atcgggccat gcccaagtga atgatccagg agtgacggca gcctacggga aggcccggtt
      421 caggtcgtcc agatcctgat ccgtcaaccg caatgacagt gccgaggcat tctgccgcac
      481 atgcgctggg gcgccggatt ccgggatggc gatgacgtgc ccgctccgga gggtccaggc
      541 gagggcgacc gccgctggac tgctctggta ccgatccgcg acctgggcca gcgccggatt
      601 ccggaggagg cccccgcccc ggccgagggg agaataggcc ataatcggca cgcgatgctg
      661 ctcgcaccag gggagcaggg cccgctcgat gcgccgatcc gtcaggttgt agcgcacctg
      721 gttggtggca taggcggtgc cgcctgggac gcggtacagt ctttccatgt ccgccacgtc
      781 gaaattggag acgccccagc gccggatgga cccctccgcc cgcagccggt cgaacgtctt
      841 caccaccacg ccgaggtccg acaccccatt aggccagtgc agcaggtaca gatccagata
      901 gtcggtcccc agacgcccca gactcgcggc acaggcctgc cggatgccgg tagcagtggc
      961 gtgggagggc cacaccttgg agaccaggaa cacgtggtcg cgctgcccgg cgatgacccg
     1021 gccgatcatc tgctccgcgc gcccgttccc atacagttcg gccgtgtcca ggagggtcaa
     1081 ccccagggag atgccgagcc ggagcgcgtc ctcctcctcc gacgccgggt ggcgaccctg
     1141 ggcgagagtc gcggagcctt gccccagggc tgggacgcga ctgccatcgg gtagctggac
     1201 gctcg
//
LOCUS       DBNGZC010000574         4377 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_147437, whole genome shotgun sequence.
ACCESSION   DBNGZC010000574 DBNGZC010000000
VERSION     DBNGZC010000574.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_147437"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="AC16G0_10045"
     CDS             <1..510
                     /locus_tag="AC16G0_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HIR5099992.1"
                     /translation="SIMPGKVNPVMAEVVNQVAFRVIGNDHTICLASEAGQFELNVME
                     PVLVFSLLESLSIMANVFRVFRKYCVEGIQPNVERMTQYVEQSVGVITAINPHVGYET
                     AARIAREAILTGKPVRELVLRDGVLNEERLREILDPYQMTRPGIAGGKAPKAERPHWR
                     ADDEAEKAS"
     gene            654..2258
                     /locus_tag="AC16G0_10050"
     CDS             654..2258
                     /locus_tag="AC16G0_10050"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012970347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HIR5099993.1"
                     /translation="MQEHSPFPHSVVGLDRQGLKNLKAVYWNLPTPALYEEALKRNEG
                     ILAHLGPLVVRTGEFTGRSPNDRFIVEEPSSRDKIWWGAVNRPIAAERFEELHRRMAS
                     YLQGKDIFVQDCWCGADPDYRMPVRIITQYAWHSLFVRNMFIQAKPEELRTHTPEFVV
                     IDCPGFHAIPEVDGTHSDIFILVHFGKGLVLIGGTEYAGEMKKSVFTAMNYFLPFEDV
                     MPMHCSANVGAGGDVALFFGLSGTGKTTLSADPSRTLIGDDEHGWSRNGVFNFEGGCY
                     AKVIRLSAQAEPEIYDTTRRYGTILENVGIDMDTRRLDLDDATLTENTRAAYPISHIP
                     NTSRDGRAGHPSHIIFLTADAFGVLPPLAKLTPDQAMYHFLSGYTAKVAGTERGVVEP
                     KATFSACFGAPFMAQHPNVYAAMLGRKIAQHKATCWLVNTGWTGGPYGAGTRMKIAYT
                     RAMINAALEGKLDGVPFAPDPVFGVLVPSSCPGVPVEVLTPRSTWADPKAYEPQARKL
                     ARMFVENFAQFGAEASDAVKAAGPKP"
     gene            2313..3041
                     /locus_tag="AC16G0_10055"
     CDS             2313..3041
                     /locus_tag="AC16G0_10055"
                     /inference="COORDINATES: protein motif:HMM:NF024258.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR5099994.1"
                     /translation="MRVAFISDIHGNFPALCGALESARRNRAGRVICAGDIVGHGPHP
                     TEVVRLLVEQKVEAILGNVDRRVLDLLQAPKKLKKRLKKKAQAPAAWAALALGDAERA
                     WLEGLPKHLRFIAGGADVWVVHGSPLSDTDYIYPSITTRALAVKLGEDRPGVLVCGHT
                     HIPFTKRIGGVRVINCGSVGRPYDGDPRGALTLCDFPGKGQVHCRILRFTYPVESLIA
                     DLRVRGAKGIEPQEYHTGSRPKDE"
     gene            3223..3600
                     /locus_tag="AC16G0_10060"
     CDS             3223..3600
                     /locus_tag="AC16G0_10060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5099995.1"
                     /translation="MRKPLIAIVDDDASVRKALERLLKAAGFEATSFASALEFLEGGL
                     AKAPECLVLDIHLGGMNGFELYEQLVTRGSTVPVIFITAHDDAPTREHARQIGSSGYL
                     RKPFEQHALLGAIYHVLKQETAP"
BASE COUNT          820 a         1396 c         1407 g          754 t
ORIGIN      
        1 tccatcatgc ctggcaaggt gaacccggtg atggccgagg tcgtgaacca ggtggcgttc
       61 cgggtgatcg gcaacgacca cacgatctgc ctcgcctccg aggcgggcca gttcgagctg
      121 aacgtcatgg agccggtgct ggtcttcagc ctgctggagt cgctcagcat catggcgaat
      181 gtcttccggg tgttccggaa gtactgcgtc gagggcatcc agcccaacgt ggagcggatg
      241 acgcaatacg tcgagcagag cgtgggtgtg attaccgcca tcaatccgca cgtgggctac
      301 gaaacggcgg cgcggatcgc ccgcgaggcg atcctgacgg gcaagccggt gcgagagctg
      361 gtgctacgtg atggggtgct caacgaggag cggctcaggg aaatcctcga cccctaccag
      421 atgacccgcc ccggcatcgc cggtggcaaa gcgccgaaag ccgagcggcc gcactggcgg
      481 gccgacgacg aggcagagaa ggcgagctga accataggcc tggagctacc acactgcggg
      541 cggggatggg ggtgcgcccg gcgccttcca catcccggtc cgggccatcg ccggcatcag
      601 cggcctgcgc atgaggcgag gcttcgacgt ctctttccag gggaggaaga gtcatgcagg
      661 aacacagtcc gttcccgcac agcgttgttg gcctggatcg ccaggggctc aagaacttga
      721 aggcggtgta ctggaatctg ccgacgccgg cgctttacga ggaagcgctc aagcgcaatg
      781 aaggcatctt ggctcacctg ggcccgctcg tcgtgcgcac gggggagttc accgggcgct
      841 cgcccaacga cagattcatc gtggaggagc cgagcagccg ggacaagatc tggtggggcg
      901 cggtgaaccg ccccatcgca gccgagcgtt tcgaggagct gcaccggcgc atggcctcgt
      961 acctgcaggg caaggatatc ttcgtccagg attgctggtg cggggccgat ccggactacc
     1021 ggatgcccgt ccggatcatc acccagtacg cctggcacag cctgttcgtc cgcaacatgt
     1081 tcatccaggc caagccggag gagctccgca cccacacgcc ggagttcgtg gtgatcgact
     1141 gccccggctt tcacgccatc ccggaggtgg acggcactca ctccgacatc ttcatcctgg
     1201 tccacttcgg caaggggttg gtgttgatcg gggggacgga gtacgcgggc gagatgaaga
     1261 aatccgtctt caccgccatg aactacttcc tgcccttcga agacgtgatg cccatgcact
     1321 gctcggccaa cgtgggggcg ggtggcgacg tggccctctt tttcggtctg agcggcaccg
     1381 gcaagaccac gctgtccgcc gatccctcac ggacgctcat cggtgacgac gagcatggct
     1441 ggagccggaa cggcgtcttc aacttcgagg ggggctgcta cgccaaggtc atccgcttgt
     1501 cggcccaggc ggaacccgag atctacgaca cgacccgccg gtacggcacg atcctggaaa
     1561 acgtcggtat cgacatggac acccggcggc tggacctgga cgatgcgacg ctgaccgaga
     1621 acacgcgggc ggcctatccc atctcgcaca tccccaacac gagtcgggac gggcgggccg
     1681 gccatcccag tcacatcatc ttcctgaccg ccgacgcctt cggggtcctg ccgcccttgg
     1741 ccaagctgac cccggatcag gccatgtacc acttcctgtc cggctacacc gccaaggtgg
     1801 cggggaccga gcgcggggtc gtggagccca aggccacctt cagcgcctgc tttggggccc
     1861 cgttcatggc ccagcacccc aacgtctatg ccgcgatgct gggacggaag atcgcccagc
     1921 acaaggccac ctgctggctc gtcaacacgg ggtggacggg tggaccctac ggcgcgggca
     1981 ctcggatgaa gatcgcctat acccgcgcca tgatcaatgc ggcgctcgaa gggaagctgg
     2041 acggggtccc cttcgcgccc gatccggtct tcggcgtcct ggtcccgtcg agctgcccgg
     2101 gtgtgccggt cgaggtgctg actccccgca gcacgtgggc cgaccccaag gcctatgagc
     2161 cccaggcgcg caagctggcc cgcatgttcg tggagaactt cgcccagttc ggggccgagg
     2221 cgtccgatgc cgtcaaagcg gcggggccca agccctgagg ctgtcggcag cgggaacccg
     2281 acgatcacgg cgacacggcg cagggagcgg ccatgcgggt agcgttcatc tccgatatcc
     2341 atggcaactt ccccgccctc tgcggggccc tggagtcggc ccgtcgaaac cgggcgggcc
     2401 gcgtgatctg cgccggcgac atcgtgggcc acggtcccca tcccacggag gtggtccgtc
     2461 tcctcgtgga gcagaaggtc gaagccatcc tggggaatgt ggaccggagg gtcctggatc
     2521 ttctgcaagc cccgaaaaag ctgaagaagc gcctgaagaa gaaggcccag gcaccggcag
     2581 cttgggcggc cttggccctg ggcgatgcag agcgcgcctg gctcgaagga ctgcccaagc
     2641 atctgcgctt catcgctggc ggggccgacg tctgggtggt tcatggcagc ccgctatcgg
     2701 acaccgatta catatacccg agcatcacca cccgggccct tgcggtcaag ctgggggagg
     2761 accgtccggg cgttctggtg tgcggtcaca cccacatccc cttcacgaag cgaatcgggg
     2821 gagtgcgcgt cataaactgc ggatccgtcg gccggcctta tgacggggac ccgcgcggcg
     2881 ccttgaccct ctgcgacttc ccgggcaaag gccaggtgca ctgccgcatc ctacggttta
     2941 cctaccccgt ggaatcattg atcgcggatc tccgggtgcg gggcgccaag ggcatcgagc
     3001 cgcaggagta ccacacggga agcaggccca aggacgagtg agagattgtg catccacccc
     3061 ttgtctcgtg ctgcctgctt cccgccgtct cgccggggag agtaggacta aggtccaata
     3121 gacggggcaa ttgcccttac gttagattct caccaatcag accgactgaa gtctcatcta
     3181 tagtctgaat ggtttcagtc gtgcgttcaa cgaggtccta ccgtgcgaaa gccccttatc
     3241 gcaattgtgg acgacgatgc atccgtccgg aaggcgttgg agaggctgct caaggcggca
     3301 ggcttcgagg caaccagctt tgcctcggca ctggagtttc ttgagggcgg gctggcgaaa
     3361 gcccccgaat gcctggtgct ggatattcat ctgggcggca tgaacgggtt cgagttgtac
     3421 gaacagctag tgacaagagg atccaccgtt ccggtcatct tcatcaccgc acatgacgat
     3481 gcccctacgc gtgagcatgc gcggcagatc ggatcctccg gatacctccg caagcccttc
     3541 gagcagcatg cgttactcgg ggcgatctac catgtgctca agcaagagac tgcaccatga
     3601 tgcttttttt ggacctctgc aaattggctc gcgaatcctt ggctgaatga ccaatccccc
     3661 gtgatcaaat tttgggggct cagcgtatcg cctaggaagg tttccatccc agcacgacct
     3721 ctcgggaaaa agggcagtag atcgcggggc gcgtgggcga cgtgacgatc accgaggcaa
     3781 cctggcacga cggcggctga agcgggagat ggaaatggga agcggctgca agggtggcgt
     3841 gctcgcaatc gcgctgttgc ttccctggca aggatcggag gcggcaacag ctccatcgaa
     3901 cgcatgtcga agcctggctg gaatctacgc cagtgcccca gagaagatgg atgagcaggc
     3961 gctggcggca ttgcaggact gcctcgccgc tgaagccggg acacaggcgg cgtccactcc
     4021 accgcccgaa gcgtcctctc agaacatccc agttgcgccc gcccctcaat cggcgggtgc
     4081 accgcaacaa gagcgcaggt acggagagtg gccagcctcc ccagcctgga ccgaacactg
     4141 gccctccccc aatccatggt agcggcgatg tcccgcccgg agcctctcgt ctctcccacc
     4201 ctctccctcg tcttgaccgt gcagctgcga tccttttctc cttccaagac acaatctcgc
     4261 cctgcattgg accaaggtcc aatagacagg ctaccggtga attgaatatc gtccctccat
     4321 ctgctgggac cagggtaccg tgtggtacga ggtctgagcg cttctgatga actcata
//
LOCUS       DBNGZC010000575         2269 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1320318, whole genome shotgun sequence.
ACCESSION   DBNGZC010000575 DBNGZC010000000
VERSION     DBNGZC010000575.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1320318"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            751..2082
                     /gene="ltrA"
                     /locus_tag="AC16G0_10065"
     CDS             751..2082
                     /gene="ltrA"
                     /locus_tag="AC16G0_10065"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HIR5099996.1"
                     /translation="MPGTPSPDAISTRRQRIAELARRSPQVAFTTLAHYIDIDWLWEA
                     YRRTRKDGAAGVDGQTATDYAADREGNLRSLLDRAKSGRYQAPPVRRVHIPKGTGPET
                     RPIGIPTFEDKVLQRAVVMVLEAIYEQDFLDCSYGFRPGRSGHQALEVLRQRLMEVRG
                     GWVLEIDIRKFFDTLDHRHLQAILRRRVRDGVLLRLIGKWLNAGVLEDGCVSHPESGS
                     PQGGVVSPVLANAYLHEVLDTWFEQTVKSRLRGRAYLIRYADDAVLVFEQEGDARRVL
                     EVLPKRFGKYGLTLHPEKTRLVPFQGLRPGAPPRPRKDRPGTFDFLGFTHYWGRTRKG
                     TWTVKRKTAGDRFRRSLKSIAEWCREHRFLSVAEQQAALTAKLRGHFTYYGITGNSRS
                     LNSFRYHVRLAWHKWLSRRSSKAYIPWERFGELERRYPLPPARLNRARPVT"
BASE COUNT          418 a          692 c          796 g          363 t
ORIGIN      
        1 ggatgcaggt ggagggaccg actccatcgg tctggcatcg ccaggacttc ctcgattggc
       61 gggatcatcg cccgcgtaat ccggtcgaac cgccgtggta cggacccgta tgcccggtgg
      121 tgtgggaggg gaggagccgc gaggctcccc cctatcccga tcaagtcggc tttagtgcgc
      181 cagggaagcc tgggaaagga ggatgcggaa gcggaaacct ttcgatggaa ccctgctggt
      241 aaccaatccc gtccggcaga gctggccagc agtcggaagc gagtcttgcg tggtgccggg
      301 gtgacccgga ctgcgaagcg tagacagcga gtgaatgggt cgtgtaatgg agcctcgaaa
      361 tcttactccc gcgggagccg acgtccttaa agcgacggaa ggccgcgtcg cggcgccgcc
      421 atggcctggc gcccgcgatc ccgccggggt cagagaacag gacacgttca cgagggttcc
      481 ccaggaacct gggaggccct gtcgtctcca cggcaaatcc cggcgggagg taccgggtga
      541 ccaactccag gcccgtggtc ggcgcactcg acggccacgg gaacgaaacg cgggtgcaac
      601 cggggtaccg ccaagcgaag gcaacgaagc gcggcgggag gggcggcagg gagtcggagc
      661 gcctcatact acctgtgacg cgggggaacc gacccgaggg accccgctga gggaaggggg
      721 cgccgggcca tgaaaccgct ggagggacag atgccaggga caccgagccc tgacgccatc
      781 tcaacgcgac gacagcggat agcggaactg gcgaggcgat cgccacaggt ggcgttcacg
      841 acgctcgccc actacatcga catcgactgg ctgtgggagg cctaccggcg cacgcgcaag
      901 gacggagccg cgggagtgga cgggcagacg gctacggatt acgcggccga ccgggagggc
      961 aacctccgat cgctgctgga ccgtgccaag tccggccgct accaggcacc gcccgtgcgg
     1021 cgggtgcaca tcccgaaggg gacgggcccg gagaccaggc ccatcggcat tccgacgttc
     1081 gaggacaagg tccttcagcg tgcggttgtc atggtcctgg aagccattta cgagcaggac
     1141 ttcctggact gctcgtacgg gttccggcct ggccgctcgg ggcaccaggc gttggaagtc
     1201 cttcggcagc ggctgatgga ggtgcggggc gggtgggtct tagagatcga catacgaaaa
     1261 ttcttcgaca ctctggatca ccgtcacctg caagcgatcc tccgccggag ggtacgtgac
     1321 ggcgtcctgc tacgcttgat cggcaagtgg ctcaacgcgg gcgtgctgga ggacgggtgc
     1381 gtgtcgcacc ccgagtccgg tagcccccag ggcggggtgg tcagccccgt cctggccaac
     1441 gcctatctgc acgaggtcct ggacacgtgg ttcgagcaga cggtcaagtc ccgcctgaga
     1501 gggcgggctt acttgatccg ctacgcggac gacgcggtcc tggtcttcga gcaggaaggc
     1561 gacgcgcgac gggtcctgga ggtgctgccg aagcgattcg ggaagtacgg cctgaccctc
     1621 catccggaga agacccggct ggtcccgttc caagggctcc gaccgggcgc gcccccgcgc
     1681 ccccgcaagg atcgccccgg cacgttcgac ttcctggggt tcacccatta ttggggtcgg
     1741 acgcgcaagg ggacttggac ggtgaagcgc aagacggcgg gagaccgctt tcgtcgatcg
     1801 ttgaaatcga tagcggagtg gtgtcgtgag caccgcttcc tctcggtggc ggagcagcag
     1861 gctgccctga cggcgaagtt gcgcgggcac ttcacgtact acgggatcac cggaaattcg
     1921 cggagcctga acagctttcg ataccacgtg aggctcgcct ggcacaagtg gctcagccgc
     1981 cgctcgagca aggcgtatat cccgtgggag cgattcgggg agttggagcg gcgatacccg
     2041 ctgccccccg ctcggctgaa tcgcgctcgt ccggtcacgt agcgaactca tggcctgaag
     2101 agccggatgc ccgaattggg cctgtccgga tctgtggggg cccgaggtgg gtgaccaccc
     2161 cgggctaccc gactgttctt ccctctcgtc cagcaaaagt gcaaaataga cactgttctc
     2221 accccgtgaa tttacgcagt atgtgcttat catattaatt agtgtttgg
//
LOCUS       DBNGZC010000576         1433 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_636249, whole genome shotgun sequence.
ACCESSION   DBNGZC010000576 DBNGZC010000000
VERSION     DBNGZC010000576.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1433)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_636249"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(510..1241)
                     /locus_tag="AC16G0_10070"
     CDS             complement(510..1241)
                     /locus_tag="AC16G0_10070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5099997.1"
                     /translation="MPLSIIFPCLNEAEGLRHCIPDAIKACDDLGLTREIIVADNGST
                     DRSASMAEAMGATVVQARPRGYGYAIQAGVRAARYPWIIMADADGSYPFGDGFALYSM
                     TKLLDLGEQVIIGNRFKGKMMPGAMPWSHRYLGNPILSGLLRLLFRAPVGDAHCGMRG
                     FTREAWEIMQCQTGGMEFASEMVVKAVRAGLRISEVRVNYHPRIGTSKLHPIRDAWRH
                     VRFLLKARVEKRPATKPAAVRSPAP"
     gene            complement(1241..>1433)
                     /locus_tag="AC16G0_10075"
     CDS             complement(1241..>1433)
                     /locus_tag="AC16G0_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008396832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HIR5099998.1"
                     /translation="EDLVKPCVLAGSPEGATVLDPFAGSGTVVKVAQDLGRHGVGIEL
                     NPDYTQLGWTRSRQRGLLL"
BASE COUNT          248 a          505 c          432 g          248 t
ORIGIN      
        1 ccgagcactt cccgacgccg ggatgtactt ccacgcggcc acgtacattg atgagtgtgg
       61 ccgccccctt ggcctcctga cggcccccct gaagtccgcc ctcgtctacc cccgaacgag
      121 cctcctcccc cagctcctcg tccagaactt catcgccatg cctaccgtgc tcgtgaaagc
      181 ctcattggcc cgattcattc atgaagacct ctggtacagc gccgattggc tgcaatggct
      241 ctggattgcc gagcaggccc ccgtcgtcta ctggcaccag ccgctcgcct cgttccgcct
      301 ccatccagcc tctcagacgg cccagcggag acaggacatc cgggggcagc tcgaactagt
      361 cttgctctgg agcagacggg acgatccggt ggcccgattc tcactggagg tgaatgtggc
      421 cctcgggggc aggccctcgt ggcgcctgct gacccaggcc ggccggttga ccttcgggga
      481 ctggtcccgg tacttccggg actccagact cagggagcgg gtgagcgcac ggctgcgggc
      541 ttggtggcgg gacgtttctc gacgcgagct ttcagcagga accgcacatg ccgccaggca
      601 tcgcgaatcg gatggagttt cgaggtcccg atccgcggat ggtagttgac ccggacctcg
      661 ctgatccgta agcctgcccg caccgccttc accaccatct ccgaggcgaa ctccatgccc
      721 cccgtctggc actgcatgat ctcccatgcc tcgcgcgtaa agccccgcat cccgcaatgg
      781 gcatcgccaa ccggcgcccg aaagagaagc cgcaacagcc cgctcaggat cgggttgccg
      841 aggtagcggt ggctccacgg catcgcgccg ggcatcatct tccccttgaa gcggttgccg
      901 atgatgacct gctcgccgag atcgaggagt ttggtcatgg aatacaaggc aaagccatcg
      961 ccaaatgggt aactcccgtc ggcatccgcc atgatgatcc acgggtaccg ggcagccctg
     1021 accccggcct ggatcgcgta gccataccct cgtggcctcg cctggacgac cgtggccccc
     1081 atcgcctcgg ccatggacgc cgagcggtcg gtggagccgt tatccgccac gatgatctct
     1141 cgagtgagac caaggtcatc gcacgccttg atcgcgtcgg gaatgcagtg tctcaacccc
     1201 tcggcctcgt tcaaacacgg gaagatgatg gagagcggca tcacagcagg agcccccgct
     1261 ggcggctgcg cgtccagccg agctgggtat agtccgggtt cagctcaatc cccacgccgt
     1321 ggcgcccgag atcctgggca accttgacca cggtgcccga gcccgcgaag ggatcgagga
     1381 cggtggcgcc ttcgggagac ccggccagca cgcacggctt gaccagatcc tcg
//
LOCUS       DBNGZC010000577         1382 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_346339, whole genome shotgun sequence.
ACCESSION   DBNGZC010000577 DBNGZC010000000
VERSION     DBNGZC010000577.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1382)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_346339"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="AC16G0_10080"
     CDS             complement(<1..370)
                     /locus_tag="AC16G0_10080"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012549.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR5099999.1"
                     /translation="MNQTEERREGEATAAETLVLQRLREQFAEALLEVKLWRNETTVV
                     LDPRSLIRVCRFLRDDPDLAFDFLSDVTGVDRLKLPQSSPRFEIVYHLYSLPHKRRLR
                     LKVRVDEGETVPTVTGVWETA"
     gene            complement(399..755)
                     /locus_tag="AC16G0_10085"
     CDS             complement(399..755)
                     /locus_tag="AC16G0_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015722616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR5100000.1"
                     /translation="MLLDYIPILILAILATLFAGANIVLSLLLGPRKPNPEKLSPYEC
                     GMVPVGTARERFSVKFYLVAMLFIVFDMEIVFLYPWAVVFKQLKLFGLIEMGIFLLIL
                     LVGYFYIWKKGGLEWE"
     gene            complement(857..1201)
                     /gene="atpE"
                     /locus_tag="AC16G0_10090"
     CDS             complement(857..1201)
                     /gene="atpE"
                     /locus_tag="AC16G0_10090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012365.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR5100001.1"
                     /translation="MGVLGVWMMWVIPGVVLLTSGSAWAAEATAGGGGLSIGAVSIIT
                     GGFAMAIASAAAAIGQGKAIVAALDGIARQPNAAPRIQVAMIIGLALIESLAIYVLLI
                     SLIVFFVKPFGG"
BASE COUNT          267 a          467 c          424 g          224 t
ORIGIN      
        1 tggcggtctc ccacacgccg gtcaccgtgg gcaccgtctc tccctcgtcc acgcggacct
       61 tcagtcgcag gcgacgcttg tgcgggaggg aatagaggtg atacacgatc tcgaagcggg
      121 gcgaggactg cggcaacttc agccggtcca cgcccgtcac gtctgagagg aagtcgaagg
      181 ccaggtcggg atcgtcacgc aggaagcggc agacgcgtat gagggaccgg gggtccagga
      241 ccacggtggt ctcgttccgc cagagcttca cctcgagcag cgcctcggcg aactgctccc
      301 gcagccgctg caggaccagc gtctccgccg cggtcgcctc gccctctcgc cgctcctccg
      361 tttgattcat cccgccctct gcgcccatgc ggttggcatc actcccactc gaggccgccc
      421 ttcttccaga tgtagaagta cccgaccagc aagatcagca gaaagatccc catctcgatg
      481 agaccgaaca gcttgagctg cttgaacacc accgcccagg gatacagaaa gacgatctcc
      541 atgtcgaaaa cgatgaagag catcgccacg aggtagaact tcacggagaa ccgctcccgg
      601 gcggtcccca ccggcaccat gccgcactcg tagggggaga gcttctccgg gttcggcttg
      661 cgcgggccga ggagaaggga caggacgata ttggccccgg cgaagagcgt ggccaggatg
      721 gccaggatca agatcgggat ataatcgagg agcatgctat gagatcatcc gacgggccga
      781 acatgtgaag cttggtacat tgggaggtca agagaggggg acctcgacgg cccgacagcg
      841 aggtccccca tgcggtttac ccgccgaagg gtttcacgaa gaagacgatc agggagatga
      901 gcagcacgta gatcgccagg gactcgatca gggccagacc gatgatcatg gccacctgga
      961 tccggggtgc ggcgttcggt tgccgcgcga tgccatccag ggcggcaacg atggccttcc
     1021 cctggccgat ggcggctgcg gcggacgcga tcgccatggc gaacccgccc gtgatgatgc
     1081 tgaccgcccc gattgacagg ccgccgccgc ccgccgtggc ctccgccgcc caggccgacc
     1141 cggacgtgag gagcacgact ccgggaatta cccacatcat ccacacgcca agcacgccca
     1201 ttcaatccct cctttctccc ctcgtctgat gtcctgccgt cgatccgcgt atcgtgattg
     1261 tgcccgcatc tccgctgacc tccggcggcg cgcggccgtc ctctgcaaag ccagctcagt
     1321 gatgctcttc cccgtgcccc tcttccaccg ccccggcgat gtacaccatg gacagcatga
     1381 tg
//
LOCUS       DBNGZC010000578         3403 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2398107, whole genome shotgun sequence.
ACCESSION   DBNGZC010000578 DBNGZC010000000
VERSION     DBNGZC010000578.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2398107"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="AC16G0_10095"
     CDS             complement(<1..318)
                     /locus_tag="AC16G0_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012466343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR5100002.1"
                     /translation="MLHSLHLCVITDAGLAPGKDHVAIAEAALRGGADMIQLRDKAGD
                     LRDLLRQARAIQALCRERGAVFIVNDRVDLALAAEADGAHVGQDDLPAEAARTLLGPG
                     RVLG"
     gene            complement(318..1661)
                     /gene="accC"
                     /locus_tag="AC16G0_10100"
     CDS             complement(318..1661)
                     /gene="accC"
                     /locus_tag="AC16G0_10100"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR5100003.1"
                     /translation="MFSKILIANRGEIAVRIIRACRELGVRTVAVFSQADAEALHVRL
                     ADEAVCIGPPPPGQSYLQVSRIISAAEITGAEAIHPGYGFLAENAHFAEVCEQCGMTF
                     IGPPSASIKLMGDKAAARRKAASEGVPVLPGSQHPVRELEEARRIAREIGYPLMVKAS
                     AGGGGKGMRVVAEPDALEPSLATAAAEAAAAFGDAAVYLERFLLDPRHVEIQILMDAH
                     GQGIHLGERDCSIQRRHQKLLEESPSPGLIGSRREAMGRAALSIAAAAGYRNAGTVEF
                     LLDERGEFYFMEMNTRIQVEHPVTEMVTGLDLVKAQVRIAAGDRLDVTQADVRFTGHS
                     LECRINAEDPSRFLPSPGRLTNIRLPGGPGVRVDSHAYVGYVMPPFYDSLLAKLIVHG
                     QDRGEAVVRMQRALDEMLIEGVQTTIPFHQKLLRHPEFLAGRTSTGFLGRLMREE"
     gene            complement(1753..2208)
                     /gene="accB"
                     /locus_tag="AC16G0_10105"
     CDS             complement(1753..2208)
                     /gene="accB"
                     /locus_tag="AC16G0_10105"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016329697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR5100004.1"
                     /translation="MDLKELKALLRLMEGNDLEELELEEGGKRVRIRRRAPESMAPRG
                     QAVSVTHPPAPSAAATPILPAGGAEAAGLIPVESPMVGTCFRAPAPGAEPYVKEGDLV
                     HKGTVLCIIEAMKLMNEIEAEVSGRIVQVPVENGHPVEFGQTLFLLEPV"
     gene            complement(2220..2777)
                     /gene="efp"
                     /locus_tag="AC16G0_10110"
     CDS             complement(2220..2777)
                     /gene="efp"
                     /locus_tag="AC16G0_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR5100005.1"
                     /translation="MILAGDLRVGTKVELDGVAYIVSESQFVKPGKGGAFTRTRLKNM
                     RTGAVIERTFRSEEKIETPELEERTAQFMYRSGDEFHFMNSETYEQFYLSEEQLGRAR
                     DYLKEEMVVTILLHQGKPLNVEVPTFVELAVAETEPGVRGDTASGGSKPATLETGAVI
                     QVPLFINVGDRLRVDTRTGAYIERA"
     gene            complement(2774..3268)
                     /gene="aroQ"
                     /locus_tag="AC16G0_10115"
     CDS             complement(2774..3268)
                     /gene="aroQ"
                     /locus_tag="AC16G0_10115"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015126106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR5100006.1"
                     /translation="MTRILVLNGPNLNLLGVREPGVYGTTTLRDIEQAVRRLADSLSC
                     EVRFQQLNGEGELVEALHAAMRWADAVVLNAAAYSHTSVALRDAIAATQLPVIEVHLS
                     NIHAREPFRHHSLTAEVAVGLISGFGSESYLLGLRAALAHLTTHPPGGRRAQQARLRR
                     SKTK"
BASE COUNT          518 a         1232 c         1075 g          578 t
ORIGIN      
        1 cccgaggacg cgtcccggac cgaggagtgt tcgcgccgcc tctgccggca ggtcatcctg
       61 cccgacatgc gcgccgtccg cttccgccgc cagggccaag tccacccggt cgttcacgat
      121 gaagaccgcg ccgcgctccc ggcacagcgc ctggatggcc cgggcctggc gcagcaggtc
      181 gcgcaggtct cccgccttgt cgcggagctg aatcatgtcc gccccgcctc gcagagccgc
      241 ctcggcgatc gccacatggt ccttccccgg cgccagaccg gcatccgtga tcacgcacag
      301 gtgcaggctg tgaagcatca ctcctcccgc atcagccgcc ccaggaagcc ggtggaagtc
      361 cgcccggcca ggaactccgg atggcgcagc agcttctggt ggaaggggat cgtggtctgc
      421 acgccttcga tcagcatttc gtccagggcc cgctgcatgc ggacgaccgc ctctccccga
      481 tcctggccgt ggacgatgag cttggccagc aaggagtcat agaacggcgg catgacatag
      541 ccgacatagg cgtggctgtc cacccggaca ccaggcccgc ccggcagacg gatgttcgtg
      601 agccgtccgg gcgagggcag gaatcggctc gggtcctcgg cgttgatccg gcactccagg
      661 ctgtgtccgg tgaagcgcac gtccgcctgg gtcacgtcca gccgatcccc ggcggcgatc
      721 cggacctgcg ccttcaccag gtccaggccc gtcaccatct cggtgaccgg atgctctacc
      781 tggatccggg tgttcatctc catgaagtaa aattcccccc gctcgtccag caggaattcc
      841 accgttccgg cgttgcggta accggcagcc gcggcgatgc tgagagccgc ccgccccatg
      901 gcctcacgtc gggagccgat cagtccgggt gagggggact cctccaggag cttttggtgc
      961 cgccgctgga tggaacagtc ccgctccccc agatggatcc cctgcccgtg ggcgtccatc
     1021 aggatctgga tctccacgtg ccgcggatcg agcaggaagc gctccaggta gaccgctgca
     1081 tccccgaagg ccgctgccgc ctccgcggcc gccgtggcca aagacggctc cagggcgtcg
     1141 ggctccgcaa ccacccgcat tccctttccg cctccgcccg cactggcctt caccatgagc
     1201 gggtagccga tctcccgggc gattcgccgg gcctcctcca gctcccggac aggatgctgg
     1261 gagcccggca gaaccgggac gccctccgag gcggccttcc ggcgcgccgc cgctttgtcg
     1321 cccatcagct tgatgctcgc cgacggcggg ccgatgaagg tcatcccgca ctgttcgcag
     1381 acctcggcga agtgggcgtt ctcggccagg aacccgtagc ccggatggat ggcctcggca
     1441 cccgtgatct ccgccgcgct gatgatcctc gacacctgga ggtagctttg gccgggcggc
     1501 ggcgggccga tgcacaccgc ctcatccgcg agccgcacgt gaagcgcctc ggcgtccgcc
     1561 tgggaaaaca ccgccacggt ccggaccccc agctcccggc acgcccggat gatccggaca
     1621 gcgatctcgc cgcgatttgc gatcaggatc ttggaaaaca ttgggagatc acgagagggg
     1681 tgggcgtgag gctgccgtgt tcgcaggatt gatggccgag gcgccgccct ggacccatac
     1741 gcggcccggc gctcagacgg gctccagcag gaataacgtc tgtccgaatt ctacggggtg
     1801 gccgttctcc accgggacct ggacgatgcg cccgctcacc tcggcctcga tctcgttcat
     1861 gagcttcatg gcctcgatga tgcataggac cgtgccctta tgaacgaggt cgccctcctt
     1921 gacgtagggc tccgcccccg gtgcgggggc gcggaagcag gtgcccacca tgggggattc
     1981 gaccgggatg agtccggccg cttcggcccc gccggcgggc aggatcggcg tcgccgcggc
     2041 cgatggtgct ggcgggtgtg tcaccgagac ggcctggccc cgaggggcca tcgactctgg
     2101 tgcacgccgc cggatgcgga cccgctttcc cccctcctcc agttccagct cctcgaggtc
     2161 gttgccttcc atcaaccgga gaagggcctt gagctccttc aggtccatca ggactcccgt
     2221 caggcacgct cgatgtaggc ccccgtccgc gtgtccaccc gcagccggtc acccacattg
     2281 atgaagagag gaacctggat caccgccccg gtctccaggg tcgcgggttt ggagcctcct
     2341 gacgcggtgt cgccgcgtac cccgggctcg gtctcagcca cggccagctc cacgaaggtc
     2401 gggacctcca cgttcagcgg cttcccctga tgcaagagga tcgtgaccac catctcttcc
     2461 ttcaggtagt cccgcgcgcg cccgagctgc tcttcggaca gatagaactg ctcgtaggtc
     2521 tcggagttca tgaagtggaa ttcatcgccg ctccggtaca tgaactgggc ggtgcgctcc
     2581 tccagctccg gggtctcgat tttttcctcg gaccggaacg tccgctcgat gaccgcgccg
     2641 gtccgcatgt tcttgagcct cgtccgggtg aaggcgccgc cctttccggg cttgacgaac
     2701 tgactttcgg atacgatgta cgccaccccg tccagctcca ccttggttcc cactcgcaaa
     2761 tcacccgcta ggatcacttg gtcttactcc tccgcagccg cgcctgttgc gcccggcgtc
     2821 cgccgggtgg gtgagtggtc agatgcgcga gggcggcccg cagacccagc aggtagcttt
     2881 ccgatccgaa gccgctgatg agcccgacgg ccacctcggc ggtcagggag tggtgccgga
     2941 acggctcgcg cgcgtggatg ttcgataaat ggacttcaat caccggaagc tgcgtggcgg
     3001 caatggcgtc ccggagtgcc acgctggtgt gcgagtacgc cgcggcgttc aggaccacgg
     3061 catcggccca gcgcatggcg gcgtgcagcg cctcgaccag ctccccctcg ccgttgagct
     3121 gctggaagcg gacctcgcac gagaggctgt cggcgagcct tcgcaccgct tgctcgatgt
     3181 cccggagcgt ggtcgttcca tacacgccgg gttcccggac gcctagaagg ttcaagttcg
     3241 gaccgttcag gaccaggatg cgcgtcatca cccgatccac accccctcta gcgccgccca
     3301 gaacacgccg ctcccatcgc ccctcatccc cgcctcaatt cccccgctcg caggactcgc
     3361 accgcctcgc ggagcgccag cagttcttcc agggcctcct gcg
//
LOCUS       DBNGZC010000579         2976 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2105588, whole genome shotgun sequence.
ACCESSION   DBNGZC010000579 DBNGZC010000000
VERSION     DBNGZC010000579.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2105588"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="AC16G0_10120"
     CDS             complement(<1..629)
                     /locus_tag="AC16G0_10120"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR5100007.1"
                     /translation="MGKRLVAILAGGLLLLGPLAVVQAAIYVYVDSEGTTHFTDAPTS
                     PEFRPLAAFGLPRGVNLTRGRYADLINAIASEHDVDPDLVKAIIQAESNFDPHARSRK
                     GAQGLMQLMPGTAGRYAVVNAYSPEENIRGGVRYLRSLHDLFPGQLHLVLAAYNAGEN
                     AILRYNGIPPYPETREYVSRVLNYYGSHGDLVLSPGVRASRRTARAQGM"
     gene            961..1755
                     /gene="mazG"
                     /locus_tag="AC16G0_10125"
     CDS             961..1755
                     /gene="mazG"
                     /locus_tag="AC16G0_10125"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HIR5100008.1"
                     /translation="MAESANQLFTELVEIMARLRGENGCPWDREQTSESIKPYLVEET
                     YEVLEAIDEQDPAKLREELGDLMLQIVFHAQMAEEAGTFSIADVLAGINDKLRRRHPH
                     VFGDVKAETAQEVLFNWEQIKKAERQKAQGKASLLDGVPRELPALLRAHRLQEKASRV
                     GFDWKEAHEVLVKVEEEFAELRAAMEGQAVERVEAELGDLLFSLVNLSRFLAVNPEEA
                     LRKTIARFISRFRYIEEELSRRGRSLRQATLQEMDALWSEAKTRGH"
     gene            1912..>2976
                     /locus_tag="AC16G0_10130"
     CDS             1912..>2976
                     /locus_tag="AC16G0_10130"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF018444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HIR5100009.1"
                     /translation="MVASRHPIQKPAADSALASIFGPAGILRAALPDFEDRPAQLQMA
                     EAVERAILDGETLLAEAGTGTGKTLAYLIPALLHGAKIVVATGTKTLQEQLFFKDIPL
                     LASALPRRFAASLMKGRGNYLCRRNLRRALEETHTRPKRQALLKIQKWSAASLRGDRA
                     ELDFLSENDPLWDDIAAWSETCLGSACEDYDDCFLTRMRQEAAAADLVIVNHHLLLAD
                     AVLRDSTLFQIIPSCDVLILDEAHLLEDVATDFFGDEISNFRVERLIRDTNREWTTAA
                     LVDRAIPSHLTRLAESCTRFFRALDLPEGTRRLRPGALEGPCEAAGAQLIEDLILLHD
                     LVHAVREIPEGLTACARRALE"
BASE COUNT          559 a          961 c          932 g          524 t
ORIGIN      
        1 tgcatccctt gggcgcgggc ggtccggcgc gacgcccgga cccccgggct taggacgaga
       61 tccccgtgac tgccataata attgagaacg cggctgacgt attcccgcgt ctccgggtag
      121 gggggaatcc cgttatatcg caggatggcg ttctccccgg cattgtaggc cgcaagcacc
      181 aggtgcagtt ggccggggaa caagtcatga agggagcgaa ggtaccgaac acccccgcgg
      241 atgttttcct cggggctgta cgcattgacc acagcgtacc gtccagcagt acccggcatg
      301 agctgcatga ggccctgggc gcccttgcgg gaacgggcat gcgggtcgaa attggactca
      361 gcctggatga tggccttgac caaatccgga tccacgtcat gctccgaagc gatggcgttg
      421 atcaaatcgg cataccgtcc gcgggtgagg ttcacccctc gggggagccc gaaggcggcc
      481 aggggccgaa actctggact cgtcggggca tcggtgaagt gggttgtccc ctcggagtct
      541 acgtacacgt agatggccgc ctggaccacg gccaagggtc ccagcagcag gagtcctccg
      601 gccaggatag ccaccagtct cttgcccata gtatgttccc tatagcagac cgttcaccgc
      661 ctggcaacat tgaactcacc ggctggcagc tccgggtctc ggtctgcttg ggtatccgga
      721 caaatccttc tgatccaggg tcgaagacct ccgaccccgc agggtgcaac gccggagatt
      781 tctacttgcc cggcctgccc tttcctcgta tggtacgaaa tcggctaaca gccctggaca
      841 accaggcttt cacttcgaaa ggatttctga agggttcgaa gccgggctgg aggctgaccg
      901 cggatcttga ggcttgtggg acctcacgcc aacgcgcacc gaagcgggag agtgatcctc
      961 gtggctgaat cggcaaacca gctatttacc gagttggtgg agatcatggc ccggctgcgg
     1021 ggtgagaatg gctgtccctg ggatcgggag cagacgagtg agagcatcaa gccctacctc
     1081 gtcgaggaga cctacgaggt cctggaggcc atcgacgaac aggatccggc aaagctccgc
     1141 gaggagttgg gcgatctcat gctccagatc gtcttccacg cccagatggc ggaagaagcg
     1201 ggaaccttca gcatcgccga tgtcctggcc ggaatcaacg acaagctccg acgtcgccat
     1261 ccccacgtct tcggcgacgt gaaggcagaa accgcacaag aggtgctctt caactgggag
     1321 cagatcaaga aggccgagcg ccagaaagcc caggggaagg cctccctcct ggacggcgtc
     1381 ccgagggagt tgccggccct cctccgagcc caccgcctgc aggagaaggc atcccgcgtc
     1441 ggcttcgact ggaaggaggc gcacgaggtc ctggtgaagg tcgaggagga gttcgccgag
     1501 ctccgggcgg ccatggaggg tcaggcggtc gagcgagtgg aggccgaact gggcgacctc
     1561 ctgttctccc tggtgaacct cagccggttc ctcgccgtga atccggagga ggcgctccgc
     1621 aagaccatcg cgcgcttcat ctcgcgcttt cgttacatcg aggaggagct gtcccgccgc
     1681 ggccgatcct tacgccaggc cactctccag gagatggacg ccctgtggtc ggaagccaag
     1741 acgcgcggcc actagtgtac cgggttagca gttttccgat ctatcccgag gcatgagcgg
     1801 cgccccctgg tcgggatttg tctctagaat ctcgatgtat cacgctgatc ctgacccgtc
     1861 ctcccgcagg ccgaaacatc cggaccgttg atcggtcaag ggatccaaag catggtcgcc
     1921 agtcgccatc caatccagaa gccggcagcg gattctgccc tggccagcat cttcggaccc
     1981 gcggggatcc tccgggctgc cctccccgat ttcgaggacc ggccggccca actccagatg
     2041 gccgaggccg tggaacgggc catcctggac ggtgagaccc tcctagctga ggccggcaca
     2101 ggcaccggga agaccctggc ctatctcatc cccgccctgc tacacggtgc caagatcgtc
     2161 gtcgccaccg ggacgaagac cctgcaggag caactattct tcaaggatat tcccctgctc
     2221 gcctcggcgc ttccccggcg tttcgcggca agcctcatga agggccgggg gaattatctc
     2281 tgccgccgaa acctgcgccg cgccctggaa gaaacccaca cccggccaaa gcgacaggcc
     2341 ctcctgaaaa tccagaagtg gtccgccgcc tcgctgcggg gcgaccgggc ggagctggat
     2401 ttcctctccg agaacgaccc tctctgggac gacatcgcgg catggtccga gacgtgcctg
     2461 ggcagcgcct gtgaggacta cgacgactgt ttcctcaccc gcatgcggca agaggcagcc
     2521 gcggccgatc tcgtcatcgt gaaccaccac ttgctcctgg cggacgccgt cctgcgtgac
     2581 agcaccctgt ttcaaatcat cccctcctgc gacgtgctga tcctggatga ggcgcatctg
     2641 ttggaggatg ttgccacgga cttcttcggg gatgagatca gcaatttccg ggtggagcgg
     2701 ctgatcaggg ataccaaccg cgagtggacg acggccgccc tggttgatcg cgccattcca
     2761 agtcacctga ccagactcgc cgagtcctgc actcgcttct tccgggccct ggacctcccc
     2821 gaggggaccc ggcgtctccg ccctggcgcc ctggagggcc cctgtgaagc tgccggcgcg
     2881 caactcatcg aggacctgat cctgcttcac gacctcgttc atgcggtcag ggaaatcccg
     2941 gagggcctca cggcctgcgc tcggagggcc ttggag
//
LOCUS       DBNGZC010000580         2550 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2202369, whole genome shotgun sequence.
ACCESSION   DBNGZC010000580 DBNGZC010000000
VERSION     DBNGZC010000580.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2202369"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="AC16G0_10135"
     CDS             complement(<1..286)
                     /locus_tag="AC16G0_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100010.1"
                     /translation="MTEAHPELPQQETAAEREAREQEEARQAAVAAEIAQREAEAAQR
                     KAEARARRQEVEGPQAPAAWPGHARGTLRKSPLRQQSELGPPTDASTSADP"
     gene            798..1517
                     /locus_tag="AC16G0_10140"
     CDS             798..1517
                     /locus_tag="AC16G0_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5100011.1"
                     /translation="MARQRSFVLVHGAWHGGWCWRRVSDRLVAAGHRVFAPSLTGLGD
                     RRHLLNRETTLDTHVQDILGLIEAEELCEVILVGHSYGGIVITGVAAQIPQRHKQLIY
                     LDAVLVEDGDSWSSAHPPDLVVARRKAAQESRGGPILPAPPSASFGVMDADDQAWVNR
                     RMTPHPFSTYEQRMRWGGPIGNRLPKVYVECTDPALPALAAMKDSYRGKAEWSFIGIK
                     TGHDAMVTAPKELTEILLRFT"
     gene            complement(1696..>2550)
                     /locus_tag="AC16G0_10145"
     CDS             complement(1696..>2550)
                     /locus_tag="AC16G0_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR5100012.1"
                     /translation="GESGTGKELIARAIHFNSPRAAGPLVTLNCAALPEQLLESELFG
                     HEKGAFTGAVAQRKGRFELADGGSIFLDEIGDLSPALQVKLLRVLQERQFERLGSTRT
                     LAVNVRILAATHRDLEQAMREGAFREDLYYRLNVVTVQIPPLRVRRDDIPPLLDHFLR
                     RFAEKNKREVTGLTPGARDALLKYDYPGNVRELENIVERAVLLCRGHVIDTEDLPVAL
                     RPGDRRPEEPESRALPDILASIERQAIRAALERHDGVQTQAAADLGISERVLRYKMKK
                     YGLEGRST"
BASE COUNT          389 a          851 c          844 g          466 t
ORIGIN      
        1 gcggatccgc cgaggttgag gcgtccgtcg gagggccaag ctcgctctgc tgcctcagcg
       61 gactctttcg cagggttccc cgcgcatgcc cgggccacgc ggccggggcc tggggtccct
      121 cgacttcttg ccgtcgcgcc cgcgcctccg cctttctctg ggcggcttcc gcctcgcgct
      181 gggcaatctc cgcagcaact gcggcttggc gggcctcctc ctgttcgcgg gcctcccgtt
      241 ctgcagccgt ttcttgctgt ggcagctcgg ggtgtgcctc cgtcaagtgc accgcgagaa
      301 gtaaaacatc cagtccgcag tgcggacacc gtaccggctc cggggggcct tggatcgtcc
      361 ccacgttctc gcgcgcaatc gggatgcgct ttctgacccc cgccgggccc gacatgatcg
      421 accgaatccg ggagggttcg atgaagcctc tgccttcgca ctgggggcaa ttcgggttgg
      481 cgccgccaca ggggcagggg cgtcgtggtg agtggtcggg tttgagtgcc atcagcacct
      541 cccgctgtcg gtgccgcggg ttacccccaa cggtatcggt gttccctggg tcgggtgagt
      601 cccggaccgg ggcgacatgt taacaataaa gccgtcattt gacaaagggg atggcgggct
      661 gggccacccg cggggagcac gtgctattga cagtcccctt cgttcctgga tacgcttcca
      721 ccggcgctta ccctttcccc cgggagcctc ccttcccaac ccgtgcgctc gacgacgaca
      781 cctgagagga gagcacgatg gctaggcaac gcagttttgt gctcgtgcac ggcgcgtggc
      841 acggcggatg gtgttggcgc cgggtgtccg accggctggt ggctgccggc catcgcgtgt
      901 ttgccccgtc gctcaccggc ttgggcgacc ggcgccacct gttgaatcgg gagaccaccc
      961 tcgacaccca tgtccaggat attcttgggc tcatcgaggc ggaagagcta tgcgaggtga
     1021 ttctggtcgg gcacagctac gggggcatcg tgatcaccgg ggttgcggcg cagattcccc
     1081 aacgtcacaa gcagctcatc tatctggatg ccgtcctggt cgaggatggt gattcctggt
     1141 ctagtgcgca tcctccggac ctcgttgtgg cccggcgcaa ggcggcacag gaatcaaggg
     1201 gtggaccgat cctgccggcc ccgccgtcgg catccttcgg cgtgatggat gcggacgacc
     1261 aggcctgggt gaatcggcgg atgacgccgc acccgttctc cacctacgag caaagaatgc
     1321 gctggggagg gcccattggg aaccgtctcc ccaaggtcta cgtggaatgc acagatccgg
     1381 ctctgcccgc gctcgcggca atgaaggaca gctatcgcgg aaaggctgag tggtctttca
     1441 tcggaatcaa gacgggccac gacgccatgg tgacagcacc gaaggaactc accgagatcc
     1501 tgctccggtt tacgtgaagt cgcgcgtgga gtcagctcgg gcgccgctcc acaggcttac
     1561 tctggtgtgc gctgccgaac ttcaagtcgc caagccttcg cagcggctcc acctgctcct
     1621 tcaacagccc ggactcgccc tccctgggaa tgcgctagcg attcgagctg gggcgcccga
     1681 gaggtccggg actcctcacg tcgaccggcc ctccaagccg tatttcttca tcttgtagcg
     1741 caggacccgt tcgctgatac ccaggtcggc ggcggcctgg gtctggacgc catcgtggcg
     1801 ctccagggca gcgcggatgg cctggcgctc gatgctggcc aggatgtctg gcagggcgcg
     1861 agattcaggc tcctccgggc gccgatcccc cggccgcagg gccaccggga gatcctcggt
     1921 gtcgatcacg tggccgcgac agagcagcac cgcacgctcg acgatgttct ccagctctcg
     1981 cacgttgccc ggataatcgt atttcaacag ggcgtcccgg gcgcccggcg tgagaccggt
     2041 cacctcccgc ttgttcttct cggcgaacct tcgcagaaaa tggtcgagca ggggcgggat
     2101 gtcatcgcgc cgcacccgca ggggcgggat ctgtacggtc acgacattca ggcgatagta
     2161 gaggtcctcg cggaacgccc cctcccgcat ggcctgctcc aggtcgcgat gggtcgcggc
     2221 caggatgcgc acgttcaccg ccagggtcct ggtgctgccg agccgctcga actgccgctc
     2281 ctggaggacg cgaaggagct tcacctgaag ggcgggcgag agatccccga tctcgtcgag
     2341 gaagatggag ccgccgtcag ccaactcgaa ccgccccttc cgctgggcga cggcgccggt
     2401 gaaggctccc ttctcgtgac cgaaaagctc gctctcgagc agttgctccg gcagggccgc
     2461 gcagttcagg gtcaccaggg ggccggccgc ccgcggtgag ttgaagtgga tggcccgcgc
     2521 gatcagctcc ttccccgtgc cgctctcgcc
//
LOCUS       DBNGZC010000581         2222 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1517809, whole genome shotgun sequence.
ACCESSION   DBNGZC010000581 DBNGZC010000000
VERSION     DBNGZC010000581.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1517809"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /gene="ybgF"
                     /locus_tag="AC16G0_10150"
     CDS             <1..415
                     /gene="ybgF"
                     /locus_tag="AC16G0_10150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HIR5100013.1"
                     /translation="APSVADRRPSPPSASADEFFSAGATEYARQDYEGAIQRFRAFIA
                     AYPRDPRVPDARFLLGEAQFSRQRYAEAIPEFETVIRQFPESRRVPAALYRQGQARLA
                     LGDRTGCQILRDVVSRYPQSPEAASAREALSSHCP"
     gene            534..1964
                     /locus_tag="AC16G0_10155"
     CDS             534..1964
                     /locus_tag="AC16G0_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HIR5100014.1"
                     /translation="MRLVTWLYSGMMGGLGLLLLGLTASAASGAPARTAVTQGEQKDV
                     AITVYNGNLGLVRDTRELNLGPGTHEVRFMDVAAQIDPTTVHLKSLRDAAGLHVLEQN
                     YEYDLLSPQKLLDKFVGKQVKLMTPDGSQVDALLLSNNNGPIYEINGQIHLGHPGRVI
                     LPDIPENLISKPTLVWLLQNATAQPQRVEVSYLTGGITWKADYVVVLNAKDSGGDLSG
                     WVTIDNKSGATYTDAALKLVAGDVNRVRPRPEMKAMLEAAGRAAAPAPQFQEESFFEY
                     HLYSLERRTTIKQNQTKQVSLLDAADIPIQKELRFYGASQFFRSQWAQPIPNQKVSVY
                     LEIANTEKNHLGMPLPQGIVRVYKAAADKSLQFIGEDAIDHTPKDEKVRIKVGEAFDV
                     VAERTQKDWKKIASGVYETEWEIQVRNHKQEDVQVTVIEPVPGDWEVVKANLPPEKTD
                     AHTLQFKLAVPKDGKTSVNYRVRMRW"
BASE COUNT          426 a          707 c          734 g          355 t
ORIGIN      
        1 ggcgccgagc gtcgcagacc gccgcccttc gcccccctcc gcatcggcgg acgagttctt
       61 ctccgcgggt gccacggaat atgcccggca ggattacgag ggggcgatcc agaggttcag
      121 ggcgttcatc gcggcgtacc cgcgcgatcc gcgcgtgccc gacgcccggt tcttgttggg
      181 ggaagcgcag ttctcgaggc agcgctatgc agaggccatc cccgagttcg agaccgtgat
      241 ccggcagttc cctgaaagcc gacgggttcc tgccgccctt taccggcagg ggcaagcacg
      301 cctggccttg ggcgaccgga ccgggtgcca aatcctccgc gacgtggtca gccgctaccc
      361 gcagtcgcct gaggccgcct cggctcgcga ggccctctcc agccattgtc cctgacgccc
      421 cgtttcggcc tcatgttttc tgaccccgcg ggtggaactt ctcttgccac gacctgtctt
      481 actcttcaag gggagattcg ggcggccgat cgagccgcca aggaggctat gccatgcgcc
      541 tggtgacgtg gctgtattcg ggaatgatgg gtggcctggg cctgctcctg ctcggcctga
      601 cggcgagtgc ggcatcaggg gctccggctc ggacggcggt gacccagggg gagcagaagg
      661 acgtggcgat cacggtctac aacggcaact tgggtctggt gagggatacg cgagagctga
      721 acctcggtcc ggggacacac gaggtgcggt tcatggacgt ggcggcacag attgacccga
      781 ccacggttca cctcaagtcc ctccgggatg ccgcgggcct ccacgtcctg gagcagaact
      841 acgagtacga cctcctcagc ccccagaagc tcctggacaa gttcgtgggc aagcaggtca
      901 agctcatgac cccggacggc agccaggtgg atgccctcct gctcagcaac aataacgggc
      961 ccatttatga aatcaacggt cagatccacc tggggcatcc gggccgcgtg atcctccccg
     1021 acatcccgga gaacctgatc tccaagccca ccctggtgtg gctcctgcag aatgcgaccg
     1081 cgcagcccca gcgcgtggaa gtttcctatc tcacgggcgg catcacgtgg aaggcggact
     1141 atgtcgtggt gctgaatgcc aaggacagcg gcggcgacct ctctgggtgg gtgactatcg
     1201 acaacaagag cggggccacc tacaccgatg ccgcgctcaa gcttgtggcg ggagacgtga
     1261 accgcgtccg cccgcgtccg gagatgaagg cgatgctcga agcggccggc agggcagcgg
     1321 cccccgcgcc ccagtttcag gaggagtcgt tcttcgagta ccacctgtat tccctggagc
     1381 ggcggaccac catcaagcag aatcagacca agcaggtctc cctcctggac gcggccgaca
     1441 tccccatcca aaaggagctg cggttctacg gggcgagcca gttcttccgg agccagtggg
     1501 cgcagcctat ccccaaccag aaggtgagcg tctacctgga gatcgccaac acggagaaga
     1561 accacctcgg catgccgctc ccccagggga tcgtccgcgt ctacaaggcg gcggccgaca
     1621 agagcctgca attcatcggc gaggatgcca tcgaccacac gcccaaggac gagaaggtcc
     1681 ggatcaaggt gggagaggcc ttcgacgtgg tggcggagcg cacccagaag gactggaaga
     1741 agatcgcctc cggcgtctac gagaccgagt gggagattca ggtccggaac cacaaacagg
     1801 aggacgtgca ggtcacggtc atcgagccgg ttccgggcga ctgggaagtc gtcaaggcca
     1861 acctgccccc ggagaagacc gacgcccaca ccctccagtt caagctggcc gtccccaagg
     1921 acggcaagac cagcgtgaac taccgcgtgc ggatgcgctg gtaggcgggc agcggagggc
     1981 agagaactct cagcaggggc ggtcggtacc aggtttatca gccttacgtg tcgcgagcgc
     2041 ttgaacacgc cgcagcggtc cacgatcgcg gggactcgga aacagcgtca gagggccttc
     2101 tcgtacgtca cggaagattt cagccgatcc tcggggatcg agtaatcggt gcggagaatg
     2161 gcttcggggt cgggggaaag ggtgtaaccg gagcgctcga gcatcgcgcg ggccttgtag
     2221 tt
//
LOCUS       DBNGZC010000582         3937 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1224509, whole genome shotgun sequence.
ACCESSION   DBNGZC010000582 DBNGZC010000000
VERSION     DBNGZC010000582.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1224509"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="AC16G0_10160"
     CDS             <1..1309
                     /locus_tag="AC16G0_10160"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR5100015.1"
                     /translation="GDWMRTISVGILGLGTIGSGVVKLLQSHSALLDRRVGGKLRIRR
                     IAETGTPRIPVDPKLLTTDAMEVINDPQVDIVVELVGGTTIAKTFCLEAVRKGKHLVT
                     ANKALLGEHGLEIYQAAADAHVDLGFEASVCGAIPIIRSVREGLVANHIQSIMGIVNG
                     TTNYILTKMTESGQAFGDVLAEAQAKGYAEADPTLDVGGLDAAHKLQILASLAYGGYI
                     PFGEIYIEGIQKVEAVDIAFARELGYRIKLLAIAKEDQGELEVRVHPTMIPDSHLLAS
                     VGGVYNAVHIRGDSAGSLMFSGRGAGQMPTASSVVADIVEIAHNIVFHRPSRVSLLPA
                     EQMDGSLKLKPIDRIRSRYYLRVMAVDKPGVLSAITGVLGENHISIASMIQKGRAEQA
                     AVPIVMLTHEAMEGDMRKALKQINQLHVVSRDTLCIRVEGVED"
     gene            1417..2472
                     /gene="thrC"
                     /locus_tag="AC16G0_10165"
     CDS             1417..2472
                     /gene="thrC"
                     /locus_tag="AC16G0_10165"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR5100016.1"
                     /translation="MPWHGIIRQYREFLPVSAATPIVSLNEGNTPLIRADGVRRHLGL
                     ACEVYLKYEGLNPTGSFKDRGMTVAMSKAVERGARAVICASTGNTSASAAAYAARAGL
                     NAFVLIPEGKIALGKLAQAMFHGGRVLQIRGNFDDALKIVREISEKHPVVLVNSLNPD
                     RIEGQKTGAFEVCDQLGEAPAYQFMPVGNAGNITAYWRGYREYRQVGKSGRLPRMIGW
                     QAEGAAPIVRGHVVERPETIASAIRIGNPASWKGALEAIQQSQGAIDMVTDEEILEAY
                     SLLARVEGVFCEPASAASVAGLTKWSKAHDFAQDDRTVCILTGHGLKDPDTAVKSSPK
                     PVSVEAEMGVVLKAMEM"
     gene            2631..2706
                     /locus_tag="AC16G0_10170"
     tRNA            2631..2706
                     /locus_tag="AC16G0_10170"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2664..2666,aa:Gly,seq:gcc)
     gene            2824..2899
                     /locus_tag="AC16G0_10175"
     tRNA            2824..2899
                     /locus_tag="AC16G0_10175"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2857..2859,aa:Cys,seq:gca)
     gene            3210..3857
                     /locus_tag="AC16G0_10180"
     CDS             3210..3857
                     /locus_tag="AC16G0_10180"
                     /inference="COORDINATES: protein motif:HMM:NF015639.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HIR5100017.1"
                     /translation="MSDLLPAARALSDTVVRETCQGVEAAGEKISCTKGCGACCRNLV
                     AISEVEARRIRDVVDTLPEPQRSAVRIRFADARQRLERAGLLEKLHAADRWTDADYAA
                     LVGIYFQQGIPCPFLEEESCSIYQERPITCREYLVTSPPEYCARLGSEGVRRVRLPLR
                     VFNAVARWQVPPQEHFLERWVPLILAPEWAEAHSADPPPKPGLELLRELLAHLSG"
BASE COUNT          705 a         1222 c         1303 g          707 t
ORIGIN      
        1 aggcgattgg atgagaacga tcagcgtagg gatcctggga ctgggaacga tcggttcggg
       61 ggtcgtgaag cttctccaga gccacagcgc ccttctggac cgccgggtgg gtgggaagct
      121 tcgcatccgc cgcatcgcgg agacggggac gccgcggatc ccggtggacc cgaagctcct
      181 caccaccgac gccatggagg tgatcaacga tccgcaggtg gacatcgtgg tggagctggt
      241 gggcgggacc acaatcgcga agacgttctg cctggaggcc gtgcgaaaag gcaagcacct
      301 ggtcaccgcc aacaaggcgc tgctgggaga acacggtctg gaaatctacc aggctgccgc
      361 cgacgcgcac gtggacttgg gtttcgaggc cagtgtctgc ggggccatcc ccatcatccg
      421 gtcggtgcgg gagggcctgg tcgcgaatca catccagtcc atcatgggca tcgtcaacgg
      481 gaccacgaac tacatcctga ccaagatgac cgagagcggg caggccttcg gggatgtcct
      541 ggccgaggcc caggccaagg gctacgccga ggccgacccg acgctggacg tggggggtct
      601 ggacgccgcc cacaagctgc agatcctggc ctccctggcc tacggcgggt acatcccgtt
      661 tggcgagatc tacatcgagg gcatccagaa ggtcgaggcc gtggacatcg cctttgcgcg
      721 ggagctggga taccggatca agctcctggc catcgccaag gaggaccagg gggagctgga
      781 ggtccgggtg caccccacca tgatcccgga cagccatctc ctggcgtcgg tcggcggcgt
      841 gtacaacgcc gtccacatcc ggggtgattc ggccggctcg ttgatgttct ccggccgcgg
      901 cgcggggcag atgcccacgg cgtcctcggt cgtggccgac atcgtggaga tcgcccacaa
      961 catcgtcttc caccgcccct cccgcgtctc cctgctcccc gccgagcaga tggatgggag
     1021 cctcaagctg aagcccatag accggattcg gagccgctac tacctgcggg tcatggcggt
     1081 ggacaagccg ggagtcctgt cggcgatcac cggcgtcctg ggcgagaacc acatcagcat
     1141 cgcctccatg atccagaagg gtcgggccga gcaggccgcc gtccccatcg ttatgctcac
     1201 ccacgaggcg atggaaggcg acatgcggaa ggccctgaag cagatcaacc agctccacgt
     1261 ggtgtcccgg gatacgctct gcattcgagt cgagggggtc gaagattaaa tcccaatgac
     1321 aaatgacaag tgaccaatgg gcagatgcgc cgctgcggct gcgcgcccac cagcattggg
     1381 atttggtcat tggtcatttc cttggagttt catgacatgc cctggcacgg catcatccgc
     1441 cagtaccgtg agttcctccc ggtgagtgcc gcgacgccca tcgtcagcct gaacgagggg
     1501 aacacgcctc tcatccgggc ggacggcgtt cgccggcacc tcggactcgc gtgcgaggtg
     1561 tatctgaagt acgaaggtct gaaccccacg ggctcgttca aggaccgcgg gatgacggtg
     1621 gccatgagca aggcggtgga gcggggcgcc cgtgcggtca tctgcgcgtc caccggcaac
     1681 acctcggcct cggcggccgc gtatgcggcc cgcgccggcc tcaatgcctt cgtcctgatt
     1741 cccgagggga agatcgccct ggggaagctg gcccaagcca tgtttcacgg cggccgggtc
     1801 ctgcagatac gtgggaactt cgacgacgcg ctgaagattg tccgggagat ctcggagaag
     1861 catccagtcg tcctggtgaa ctcgttgaac ccggaccgga tcgaggggca gaagacgggg
     1921 gcattcgagg tctgcgacca gctgggcgag gcgcccgcgt atcagttcat gcccgtgggc
     1981 aacgccggca acatcacggc ctactggcgc gggtaccggg agtaccggca ggtcgggaag
     2041 agcggccggc tgccgcggat gatcggctgg caggccgagg gggctgcccc catcgtccgc
     2101 ggccacgtgg tggagcgccc ggagaccatt gcctccgcca tccggattgg caatccggcg
     2161 agttggaagg gcgcgctgga ggcgatccag caatcccagg gcgccatcga catggttacg
     2221 gacgaggaga tcttggaggc ctacagcctg ctggcccggg tcgagggggt gttctgcgag
     2281 ccggcctcgg cggcctcggt ggcgggactc accaagtggt caaaggccca tgattttgca
     2341 caggatgatc gaaccgtctg catcctcacc ggccacggcc tgaaggatcc ggacacagcg
     2401 gtgaaaagct cacccaagcc ggtgagcgtc gaggctgaga tgggcgtagt cctcaaggcc
     2461 atggagatgt agaaagaacg ttctgcggtt cggctgttcg acggtacggc cgttcgggat
     2521 ctgtgcagta tagccgaacc acagaacttc cgaacagccg aacgcctttg tctttagctg
     2581 gcttttccgt tgacaattct gagcctcgct tgctatcttg tgcgccgtct gcgggaatag
     2641 ctcagttggt agagcacaac cttgccaagg ttggggtcgc gggttcgaat cccgtttccc
     2701 gctccagtct tcatctggag gggttgtgag gcgattggct cgcaacccct tttcatttcg
     2761 tcggtggtgc ggccagcggg ttcgcgttga ccgctttgcc gatttgacct ctcatccagc
     2821 cacggcgcgg tacccaagag gctaagggag aggtctgcaa aacctcgatt ctgcggttcg
     2881 agtccgcccc gctcctccac catttcaagc agttatcgtg accctgctaa gcaccatcgc
     2941 ttcccatccg gacttcatgt tcggcgcttc gacggcctca tccggcactg tagccaaccc
     3001 tgcgcctttt tctcgaagtc cgtttggcct catggaaggt tcaatcgtcg tgagggttcc
     3061 acggggccgt cgcttgcgcc agcttgaggt tcctgttcat gacgccagaa ttccccagcc
     3121 ctccagaggt agccagcgcc caattcacaa ttgaaggaga gcactttcgt ctgacgttcc
     3181 gggtgacggt acccgcgggg tcggcgcagg tgagtgacct actgcccgcg gcccgcgccc
     3241 tgtccgatac cgtcgtacga gagacgtgcc aaggggttga ggcggcgggc gagaagatct
     3301 cgtgcacgaa gggttgcggg gcatgctgcc gaaacctggt tgccatctcc gaggtcgagg
     3361 cgcggcgtat ccgcgacgtg gttgacacgc ttcccgaacc gcaacgctcc gcggttcgca
     3421 tccgtttcgc cgatgcccgc cagcgattag aacgcgccgg gcttctggaa aagttgcacg
     3481 cggccgatcg gtggaccgat gcggactacg ccgcgctggt gggcatatac ttccagcagg
     3541 ggatcccctg tccgttcttg gaggaagagt catgctccat ctaccaagag cggccgatca
     3601 catgccggga atacctggtg acatcgccgc cggagtactg cgcgaggctc ggctctgagg
     3661 gcgttcggcg agtgcggctg ccgctgcggg tgttcaacgc ggtagcccgc tggcaggtgc
     3721 cgccgcagga gcacttcctg gaacggtggg tgccgctgat cctcgccccg gaatgggccg
     3781 aagcacactc cgctgacccg ccgcccaagc cgggcctgga gcttctgcgg gagctactcg
     3841 cccatctcag cggttagggg aaactggacg gtccgcacaa ttctcgttct cgattagatc
     3901 cggtggcagg cgaaaagact gcctgcgccg aatcccg
//
LOCUS       DBNGZC010000583         2852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_733370, whole genome shotgun sequence.
ACCESSION   DBNGZC010000583 DBNGZC010000000
VERSION     DBNGZC010000583.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_733370"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="AC16G0_10185"
     CDS             complement(<1..396)
                     /locus_tag="AC16G0_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100018.1"
                     /translation="MSAISRLLDEYGRLCDSAENGRRLSLWETADCGLRGETQWHGIP
                     NYTTDSGNSMPVTAECLEKMWEAVLGMDIRRFYTEADYFLEFFLRYKILKFRRFADDT
                     PLTREIPVCFGVTHEAGLLGQAIHYLPGME"
     gene            complement(912..1223)
                     /locus_tag="AC16G0_10190"
     CDS             complement(912..1223)
                     /locus_tag="AC16G0_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100019.1"
                     /translation="MASDNRVLLTVLLHHDQSKTLEEIMSHLKKTGFYRDFPPEGAEL
                     VSWVVAMSYGFIITLLVEPGAFRSVNRFMEQRAWGAFRYEVFPAYDFAPIAAKLKQDH
                     A"
     gene            complement(1397..2569)
                     /locus_tag="AC16G0_10195"
     CDS             complement(1397..2569)
                     /locus_tag="AC16G0_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR5100020.1"
                     /translation="MPFNSEGVEPGVGLALSGGGFRATLFHLGSLWRLNELGYLPKLD
                     RVSSVSGGSITAGLLAVRWGQLQWADGIATNFVAMIADPLRAFCARSIDGPAIGEGAL
                     LPWKRISDVVQGEYAAHLLGKGTLQDLLDRPRFVFDTTNFATGVSFRFSKPYAGDYRI
                     GLLPNPTFPVALAVTASSAFPPFLYPVIVEPDPAAFQRTKGADLFDQEAYRRRLVLTD
                     GGVYDNLGLETVWNRYQTILVSDAGAPFSPAPDPATDWTRQSLRAMDVATNQARALRK
                     RALIADYQAKTRMGSYWGIMTEIAEYQLPDALPVSLESTTYLSTLRTRLNPFSEEEQG
                     RLINGGYALCDAAMRKHVIPGQAVKPPRWPYPAYALNQPMSPAPSQPVAPDPPSGR"
BASE COUNT          511 a          893 c          927 g          521 t
ORIGIN      
        1 ttccatccca ggcagataat ggattgcctg gccgagcagg cccgcctcgt gggtcacacc
       61 gaagcacacg ggaatctctc gggtgagggg cgtgtcgtcg gcgaaccggc ggaacttgag
      121 gatcttgtat ctcaggaaga attcgaggaa gtaatctgcc tcggtgtaaa agcggcggat
      181 atccattccc agcaccgcct cccacatctt ctccaggcac tcggcggtga cgggcatgga
      241 attgccgctg tcggtcgtgt aattcgggat gccgtgccac tgcgtctccc cgcgcaggcc
      301 gcagtcggct gtttcccaga gggacagcct ccgtccgttc tcggcgctat cgcagagcct
      361 tccgtactca tcaagaagcc tgctgatcgc tgacacgggt acgccttcgc ctgagatgga
      421 gagcccattc agcacgcatg cccagctttt ttccaaggat tgcttgtgag ggtgagatct
      481 gatatctcag atggcagtct cgtgtcaagg gaatagtagg atggccatac ggacaacctg
      541 cagaggagtg gagatcggta caactccggc cgtgcaacag ccgccaggat tcgcgcccct
      601 gccggcgact acacccagag gagttccgga ctcgcgccaa cgtcagccac ccgcgagcga
      661 gcgatcacgg cgcgggaaag ccgatcgcac cccagctact caattcgagc ctgccagcgg
      721 gttagactgc cccgcgggcg ttgcgccgac aggtcgagag ttcacgtctg gtgccgcagt
      781 cgagggactg ccgcccgttc cgatcgactc cgccaccttg ggggaggcgt ccatctggat
      841 ggcctgtccc ttcaccctct gccgggaggc aacgaggcgc ggcgaagacc atcaccttgg
      901 gtgggatctc ctcaggcgtg gtcctgcttg agcttggcgg cgatgggggc gaaatcgtag
      961 gcggggaaaa cctcgtagcg gaaggcgccc caggcccgct gctccatgaa gcggttcacg
     1021 ctccggaagg cgccgggctc caccagcagc gtgatgataa agccgtagga catggccacc
     1081 acccaggaca ccagttccgc gccctccggt ggaaagtcac ggtagaagcc ggtcttcttg
     1141 aggtggctca tgatctcctc aagcgtcttg gattggtcgt ggtggagtaa taccgtcagc
     1201 aggacccggt tgtcggatgc catggccgtc ctcctcaatg gcggtcaatt ggagttcgcc
     1261 tcaccgagag gcgtgatgca acctgcgtcg cccggcgcgg ccgagtcaag agatcttgcg
     1321 actcgactac gcacggctcc cccccccggg ccttcgccga tccccggtca tgcctcctca
     1381 gtgccgggcc cttgggttat cgccctgacg gggggtcggg ggccaccggc tgggatggcg
     1441 ccggggacat cggctggttg agtgcatagg ctggataagg ccaccggggt ggcttcaccg
     1501 cctgaccagg gatcacgtgt ttccgcatgg cggcgtcaca gagggcgtag cccccgttga
     1561 tcaggcggcc ctgctcctcc tccgagaagg ggttgaggcg ggttcgcagc gtcgaaaggt
     1621 atgtggtgct ctccagggag acagggaggg cgtctgggag ttggtactcc gcgatctccg
     1681 tcatgatccc ccagtacgat cccatccggg tcttggcctg gtagtctgcg atgagcgccc
     1741 gcttgcgcag ggctcgcgcc tggttcgtgg ccacgtccat cgctcggagg gactgcctcg
     1801 tccagtcggt agccggatcg ggcgccgggc tgaatggcgc tccggcgtcg ctcacgagga
     1861 tggtctggta ccggttccag acggtctcca ggccgaggtt gtcgtagacc cccccatcgg
     1921 tgaggacgag gcggcggcgg taagcttcct gatcgaagag gtcggctccc ttggtccgct
     1981 ggaatgccgc ggggtcgggt tctacgatga cggggtacag gaagggcggg aaggcgctgg
     2041 aggcggtcac ggcgagggcg acagggaagg tggggtttgg aagcaggccg atccggtaat
     2101 ccccggcgta aggcttggag aagcgaaagc tcacccccgt ggcgaagttc gtggtgtcaa
     2161 aaacgaagcg gggtcggtcg agtaggtcct ggagggtccc cttccccagc aggtgcgccg
     2221 catattcccc ctgcaccaca tcactgatcc gcttccaggg gaggagcgcc ccctccccaa
     2281 tggccgggcc gtcaatgctc cgggcgcaga aagcccgcag agggtctgcg atcattgcaa
     2341 caaagttcgt ggcgattcca tctgcccact ggagctgtcc ccaccgtacc gccaggagcc
     2401 ccgctgtaat tgagcccccc gagacgctgg agacacggtc gagttttgga agatacccca
     2461 gctcgttcag ccgccagaga ctccccaggt gaaagagggt ggcccggaag cccccgccgg
     2521 agagagccag gccaacgccg ggctctacgc cttcgctgtt gaatggcatt gccctcctcc
     2581 tcaggcacag aggccctgtc gagactcagg ctcggcgatc agatcgacca tgaccacctc
     2641 cgggatcgtt gcaacgtctc ggtcgcatac tccgtctggc cccgcgatcg gatctagcct
     2701 tgcgagaaaa ctcctgccgc gaatgacgga cagtgtcagg gcgagcaaga tgggcgctcc
     2761 aacctaggct tccttccatg agttcctgca acgtgtcagg gccccaatga cttctcgcta
     2821 gaattccacc gtgcaaagtc cgccataccc tt
//
LOCUS       DBNGZC010000584         2180 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1712705, whole genome shotgun sequence.
ACCESSION   DBNGZC010000584 DBNGZC010000000
VERSION     DBNGZC010000584.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1712705"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1152
                     /gene="rbsK"
                     /locus_tag="AC16G0_10200"
     CDS             223..1152
                     /gene="rbsK"
                     /locus_tag="AC16G0_10200"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HIR5100021.1"
                     /translation="MKSDICVVGGINMDLVVQVPHIPRPGETVHGGDVARFPGGKGSN
                     QAVAASRLGAAVAMIGQVGADAFGDELLATLREAGVRADGVGRVSGLATGVALISVAP
                     NGENAIVVSPGANMAWDEGALTDVERAAAECRLVVLNLEVPSPVIARAVRAARRAGAR
                     VILNPAPHRKGDEACFGDVDFFVPNQVEAALFAGREPDSVEDWEDIGRRLCGLGPRAT
                     ILTLGSQGSLIVSGEGTTHVPGFKVPVVDTTAAGDAFVGGLATALLRGATLSGAVRYA
                     NACGGLAVTKAGAQPSLPHRADVEELLARAERS"
     gene            1157..>2180
                     /locus_tag="AC16G0_10205"
     CDS             1157..>2180
                     /locus_tag="AC16G0_10205"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5100022.1"
                     /translation="MAGDKVRVGIIGAGRIADMVHVPSLKLCPDICEIKAVASRRPDK
                     AKAFAERWGIPRPYPDWQSLLADPEVDAVVVCPPSGLTAEVARAAIAANKHILCEKPL
                     GLSHAEAALVEADAKASPCVHMVAFTFRFVPALRYLKRLVSQGHFGEIRHWRFSYFTD
                     FMLDPSAPVAWRNQRAQAGAGILADMGSHGIDFARYLLGDITAVSGASRLYVRERPSA
                     SGAGMAPVDAEDACAFTLEFAGGAIGTFDINRAVAGRGGKGRANYQCIEIHGTGGAAI
                     YELIRPYELQFSLGPAMTAAQQWTQAEVPFDLRKYPGSPRNTRVDDPLLGYKFDQGVA
                     FLQAIRG"
BASE COUNT          350 a          664 c          820 g          346 t
ORIGIN      
        1 cgagagcggg ccaactctag tggccgttgg aggcacgtgt caaggggatg tcggggcacg
       61 gggcgcaggg gaagaggcac agggcacagc gcacagggca cagcgcacag ggcaaggggc
      121 cggggggtga gagcgcgagc gagccagcat cggaattgcg atgcgtgaca tgctgtgata
      181 tgaaggcaag gccataggag gagaacggga ggcctcgggg gcatgaagag cgacatctgc
      241 gtggtcggcg ggatcaacat ggatctggtg gtgcaggtgc cgcacatccc gcgccctggg
      301 gagaccgtcc atggcggaga cgtggcccgg ttccccgggg gcaaggggtc gaaccaggcg
      361 gtagcggcga gccgtttggg tgcggccgtc gccatgatcg gccaggtggg ggcagatgcg
      421 tttggggacg aactgctggc gacactccgg gaggcaggcg tcagggccga cggcgtgggc
      481 cgggtctccg gtctggcgac cggggtcgcc ctgatctcgg tggcgcccaa cggagagaat
      541 gccatcgtgg tctcgcccgg ggcgaacatg gcgtgggatg agggcgcact cacagatgtg
      601 gagcgggccg cggcggaatg ccggctggtg gtcctcaatc tggaggtgcc ctcgcccgtc
      661 atcgcccgag ccgtccgtgc cgccaggcgg gcgggggctc gggtcatcct gaatccggct
      721 ccccatcgga agggagacga ggcctgcttt ggcgacgtag atttcttcgt ccccaaccag
      781 gtggaggctg ccctcttcgc aggcagggag cccgactcgg tggaagactg ggaggacatc
      841 ggccgccggc tctgtggact ggggccgcgg gccaccatcc tgacgctggg atcacagggg
      901 tccttgatcg tgagcggcga gggcacgaca cacgtgcccg gcttcaaggt tccggtcgtg
      961 gacaccaccg cggcgggaga tgctttcgtc ggaggactgg ccaccgcatt gctccggggg
     1021 gccacgcttt ccggagccgt ccggtacgcc aacgcctgcg gggggctggc agtgaccaaa
     1081 gcgggcgcgc agccgtccct gcctcatcgg gcggacgtcg aggaactgct cgctcgcgcg
     1141 gaaaggagct gaggtgatgg cgggcgacaa ggtgcgggtg ggcatcatcg gcgccgggcg
     1201 cattgccgac atggtgcacg tgccgtctct caaattgtgc cctgacatct gcgagatcaa
     1261 agccgtggcg tcgcgcaggc cggacaaggc caaggccttc gccgagcgct gggggattcc
     1321 gcgcccgtat cccgactggc agtcgctcct ggccgatccg gaggtggatg cggtggtggt
     1381 ctgcccgccc agcggcttga cggcggaagt ggccagggcg gccatcgccg ccaacaagca
     1441 catcctgtgc gagaagccct tggggctgag ccatgccgag gcggccctgg tcgaggcaga
     1501 tgccaaggct agtccctgcg tccacatggt ggccttcacc ttccggttcg tcccggcgct
     1561 ccgctacttg aagcggctcg tcagccaagg ccatttcgga gagatccgcc actggcgctt
     1621 ttcctatttc acggacttca tgctggatcc ctccgcgccc gtagcgtggc gaaatcagcg
     1681 ggcgcaggcg ggggccggga ttctggcgga catgggttcc catggcatcg acttcgcccg
     1741 gtacctcctg ggcgacatca ccgccgtgag cggcgccagc cgcctgtatg tccgcgagcg
     1801 gccgagcgca tccggcgcgg ggatggcgcc ggtggatgcg gaagatgcct gcgcgtttac
     1861 cctggagttt gccggcgggg ccatcgggac attcgatatc aatcgggccg tggccggccg
     1921 cggcggcaag ggccgtgcga actatcagtg catcgagatc cacgggacgg gcggggctgc
     1981 catctacgag ctcattcgcc cctatgagct gcagttcagt ctcggaccgg ccatgacggc
     2041 tgcccagcag tggacccaag ccgaggttcc cttcgacctg cgcaagtacc cgggctctcc
     2101 ccggaatacg cgggtggacg atcccctttt gggatacaag ttcgaccagg gagtggcctt
     2161 cctgcaggcg atccggggag
//
LOCUS       DBNGZC010000585         3214 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_931474, whole genome shotgun sequence.
ACCESSION   DBNGZC010000585 DBNGZC010000000
VERSION     DBNGZC010000585.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3214)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_931474"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="AC16G0_10210"
     CDS             <1..595
                     /locus_tag="AC16G0_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100023.1"
                     /translation="PGKYTYCIAENEEASPFAPYHVEKGYRPEDSTVFLIAAEPPHSV
                     TNHVANDPEGILDSLCSAMSTIAHNTAVSGGHCAVVLGPEHARAIAKAGWTRHDIRSS
                     LWTYSGNRFGDVAFNHRYGKVYNRNLPRWYKRDLEARIPVVASPDYIHLFVAGGEAGR
                     FSAFSPGWGHMSTPVLRSIQGSGPATGPECVDGTCVV"
     gene            621..1172
                     /locus_tag="AC16G0_10215"
     CDS             621..1172
                     /locus_tag="AC16G0_10215"
                     /inference="COORDINATES: protein motif:HMM:NF049802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:924..926,aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HIR5100024.1"
                     /translation="MREGGGGQGQVVYDPRGVVEAKLQELAPRATSLDGLRLGVLDNA
                     KWNASKLLRQSVELLRKEIEFAVVNSYRKESFSRSAEPRLIQEIAGQNDVVLTAIGDU
                     GSCTSCSVHDAVALESWGIPVAMVITSAFTQEAEFQRRALGMDALRSVVITHPLSSLT
                     MEEIQSRAEEVAAQAVKVWLGQI"
     gene            1333..2970
                     /locus_tag="AC16G0_10220"
     CDS             1333..2970
                     /locus_tag="AC16G0_10220"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012269216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HIR5100025.1"
                     /translation="MKELEEQRRAWAAELEKRRPAPALTPSGIPLEPLYGPESAEERD
                     PSEIGYPGGYPFTRGLHATMYRGRLWTFRQYAGFGTAEDTNARYKFLLEQGQTGLSVA
                     LDLPTQMGYDSDDPRVEEEVGRVGVAIDTLADMEVIFQGLPLDKISTSFTINGTAAIL
                     LAMYLALCERAFVPKEKINGTIQNDILKEYVSRGTWIYPTGPSMRLIVDTIQHCAAHA
                     PNFNAISIAGAHFRDAGATAVQEMGYTLANAIAYLQACVDRGLPVDAIARQISFFFYT
                     HMDFFEEVAKYRAGRRLWAKLMRERFGAKDPRSWMFRFGCVCGGSTLTVQQPLNNVVR
                     VAYEALAAVLGGVQSMFTTAYDEAFALPTQESAELALRTQQVLAYETGVPSVADPLGG
                     SWFIEALTDKMEAEIAALIQRVEKMGGMVAAIEAGLIQREIAQEAYRQQQRVESGEKV
                     VVGVTRFTRPEKPTLALYQPDPEILGRQQARLAHVKAQRDAGRVAKALRELKEAAQGT
                     ANLMPCLIECVQAYCTVGEINGNLLEVFGRFQEPVKF"
     gene            2980..>3214
                     /locus_tag="AC16G0_10225"
     CDS             2980..>3214
                     /locus_tag="AC16G0_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007072911.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HIR5100026.1"
                     /translation="MTTRRLRVLIAKPGLDGHDRGAKVVAYALRDAGMEVIYTGLHQT
                     VEQVAAAALQESADVVALSILTGAHLSLTRKLIA"
BASE COUNT          588 a          999 c         1109 g          518 t
ORIGIN      
        1 tccgggcaag tacacctatt gcatcgccga gaacgaggag gccagcccgt ttgcgcccta
       61 ccacgtcgag aaggggtacc ggcccgagga ttcgaccgtg ttcctgatcg ccgccgagcc
      121 tcctcacagc gtgacgaacc acgtggcgaa cgaccccgag ggaatcctgg acagcctgtg
      181 ctcggccatg agcacgattg cgcataatac tgcggtcagc ggggggcact gcgccgtcgt
      241 gttgggtcca gagcacgcgc gcgccatcgc gaaggcgggc tggacgcggc acgacatccg
      301 gtcctctctc tggacctact ccggcaatcg cttcggcgac gtggcgttca atcaccgcta
      361 cgggaaggtc tacaaccgga atcttccgcg atggtacaag cgcgacctgg aagcccgcat
      421 ccccgtcgtc gccagccccg actatatcca cctgttcgtg gccggcgggg aggctggccg
      481 attttcggcg ttcagtcccg gctggggcca catgtccaca ccagtgctcc gttcgatcca
      541 ggggagtgga ccggccacag gcccggagtg cgtggacggc acatgcgtcg tatgagcggc
      601 cgcaactgac ggagggggag atgcgcgagg gtgggggagg acagggacag gtcgtctacg
      661 atccccgagg tgtggtcgag gcgaaactgc aagaactcgc gccacgggct acgtccttgg
      721 acggtctgcg gctgggtgtc ctcgacaacg caaaatggaa cgcgagcaag ctcctgcggc
      781 aaagtgtgga gctgcttcgg aaggagatcg aattcgcggt ggtcaactcc taccggaagg
      841 aaagcttttc ccgaagcgcc gaaccgcgcc tcatccagga gattgctggg cagaacgacg
      901 tcgtcctgac cgccataggc gactgaggat cctgtacgtc atgcagtgtg catgacgcgg
      961 ttgcgctcga gagctggggg attccggtcg cgatggtgat cacgtcggca ttcacgcagg
     1021 aggctgaatt ccagcgtcgg gcgctcggca tggacgcact gaggtctgtg gtcatcaccc
     1081 atccgctgag ctcgctgacg atggaggaaa tccagagtcg ggccgaggag gttgcggccc
     1141 aggcggtgaa ggtgtggctc ggtcagatct gacgggagcg ccgaggaaga tccggacggc
     1201 cggcgatggc attcaggcca gcggttcgga taggaccgag ttcctgcccc aggaggctag
     1261 ccggctcgac agcgacgtat ctcgaaccgg cctggtgcga cggctcgcaa ccgctgtgga
     1321 ggggggaggc tcatgaagga actggaggag cagcgtcgcg cttgggcggc ggagctggag
     1381 aagcgacggc ctgcgcccgc cctcacgccc tcgggaatcc cgctggagcc gctctacgga
     1441 ccggagtcgg cggaggaacg tgatccgtcg gagatcgggt acccgggcgg ataccccttc
     1501 acccggggcc ttcacgcgac catgtaccgg gggcgcctct ggaccttccg ccagtatgcc
     1561 ggcttcggga ccgccgagga caccaacgcc cgttacaagt tcctcctgga gcagggccaa
     1621 acgggcctca gcgtggccct ggacctgccg acccagatgg gatacgactc cgacgatccc
     1681 cgggtggagg aagaggtggg tcgggtcggc gtggccatcg ataccctggc ggacatggag
     1741 gtgatatttc aggggctgcc gctcgacaag atcagcacct ccttcacgat caacggcacc
     1801 gcggccattc ttctggccat gtacctcgcg ctctgcgagc gcgccttcgt ccccaaggag
     1861 aagatcaacg gaacgatcca aaacgacatc ctcaaagagt atgtgtcccg ggggacctgg
     1921 atctacccga cgggcccctc catgcgcctg atcgtggaca ccatccagca ctgtgccgcc
     1981 cacgccccca acttcaatgc gatcagcatt gcgggagcgc acttccggga tgcgggtgcc
     2041 actgccgtgc aggagatggg ctacaccctg gcgaacgcca tcgcctatct ccaggcctgc
     2101 gtggaccggg ggctccccgt ggatgccatc gcccggcaga tcagcttctt cttctacacg
     2161 cacatggact tcttcgagga ggttgccaag taccgggcgg ggcggaggct ctgggcgaaa
     2221 ctcatgcggg agcgtttcgg agccaaggat ccccgctcct ggatgttccg gttcggctgc
     2281 gtctgtggcg ggagcacgct gaccgtgcag caaccgctga ataacgtggt ccgcgtggcc
     2341 tacgaagccc tggccgccgt gctgggcggg gtccagtcca tgttcaccac ggcctacgac
     2401 gaggccttcg cccttcccac gcaggaatcg gcggagctgg ccctccggac gcagcaggtg
     2461 ctggcgtacg agactggcgt gccgtccgtg gccgatccgc ttggcggctc ctggttcatc
     2521 gaggcgctga cggacaagat ggaggcggag atcgcagccc tgattcaacg agtcgaaaag
     2581 atggggggga tggtggccgc catcgaagcg gggctgatcc agcgggagat tgcccaggag
     2641 gcgtaccgtc agcagcagcg ggttgagtcg ggcgagaaag tagtcgtggg cgtcacccgg
     2701 ttcacccggc cggagaagcc gacgctcgcc ctctaccagc ccgacccgga aatcctcggt
     2761 cgccagcagg cgcgcctggc ccatgtcaaa gctcagcggg acgcgggccg cgtggcgaag
     2821 gcgctccggg agctcaaaga agccgcccag gggaccgcca acctgatgcc ctgcctgatc
     2881 gagtgcgtgc aggcctactg cacggtgggc gagataaacg gaaacctgct ggaggtcttc
     2941 gggcggttcc aggagcccgt gaagttttag gggagtggcg tgacaaccag gcggctcagg
     3001 gtcttgatcg ccaagcccgg tctggacgga cacgaccgcg gggccaaggt ggtggcgtac
     3061 gcactccgag acgccggcat ggaggtgatc tacaccggat tgcaccagac ggtggagcag
     3121 gtggcggcgg cagccctgca ggagtcggcg gatgtcgtgg cgctcagcat cctcaccggg
     3181 gcccacctct ccttgacgcg gaagctgatc gcac
//
LOCUS       DBNGZC010000586         2728 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2106113, whole genome shotgun sequence.
ACCESSION   DBNGZC010000586 DBNGZC010000000
VERSION     DBNGZC010000586.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2106113"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="AC16G0_10230"
     CDS             <1..682
                     /locus_tag="AC16G0_10230"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF013012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0061711 - tRNA
                     N(6)-L-threonylcarbamoyladenine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR5100027.1"
                     /translation="AASRLEYPSLTHPFVALVVSGGHTHLYHVPAECEYRLLGRTRDD
                     AAGEAFDKVAKLLGLPYPGGPAIEREARGGNPSAIPFPRAVFSDGSLDFSFSGLKTAV
                     AHYLKGLERNRPGPGKPALDPQAVRDVCASFQQAAVDMLTEGVMRAIRDLGVGQLVVA
                     GGVACNTALRQALKEEAEAEGIQLCVPSPALCTDNAAMIAVAGHIRLRRGERAGWDLN
                     AVANLPLA"
     gene            732..1250
                     /gene="def"
                     /locus_tag="AC16G0_10235"
     CDS             732..1250
                     /gene="def"
                     /locus_tag="AC16G0_10235"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR5100028.1"
                     /translation="MSISTHTRPILTYGQPILRQAARPVRSIDGELQQLIDDMVETMY
                     AAPGVGLAANQVGSLQRVFVTNPSEEHDPGKLLVLVNPELVESEGEVVAEEGCLSIPD
                     FREEVRRARRVLLRGLDRNERPVEIEAQDLLARIFQHELDHLNGVLFVDRLSPAKRDI
                     LLRKLRKTFLER"
     gene            complement(1307..2341)
                     /locus_tag="AC16G0_10240"
     CDS             complement(1307..2341)
                     /locus_tag="AC16G0_10240"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR5100029.1"
                     /translation="MTGTIWVVALGLVAPLTVAPVLAQTAIPEAQPKGMILDQVVAAV
                     NDEALTLSEVQEEGQPVIRKIFRDFVGPERDRRVEEAEKRLLDELIDRRLAYQVAKKE
                     GMLPSAADVTNAIEELKKNNNVSDDAQFRALLRAEGMTFEQVRRSVEERLAISRVLSR
                     QIRSSIIIREEDLTQYYQDHQDKYQRTPDAEIRHILISVPPGADETAAKARAEEILGK
                     VRSGADFAKMAEQYSDSPTREKGGELGTIHKGDLAPEIEAAAFSLPVGGVSDLIRTSA
                     GWNLIQVERVRTETVAPFAEVRDTIRDELFQEKFEAKRKEWLAGLRAKAFIQVIMEAG
                     ALKTELQKTK"
     gene            complement(2338..>2728)
                     /locus_tag="AC16G0_10245"
     CDS             complement(2338..>2728)
                     /locus_tag="AC16G0_10245"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR5100030.1"
                     /translation="SKDTSTAAKGGDLGFFSRDQMVQDFAKAAFALKPGEVSGVVKSP
                     FGYHVIQVLDRQKGQPLTFEQVKAQLQRRLLDESQNQRFTQWIKDLEGAAKITRDESL
                     LPVGTPLGPPPGAPSPGTGGAKTGDKS"
BASE COUNT          433 a          917 c          883 g          495 t
ORIGIN      
        1 cgcggcttcc cgactggaat atccgagtct cacgcatccc ttcgtggcgc tggtggtctc
       61 gggcggccac acccatctgt accatgtccc ggccgagtgc gaataccgtc tcctgggccg
      121 gacccgagat gatgccgccg gggaggcctt tgacaaggtc gccaagctcc tggggcttcc
      181 atatccgggc ggtcccgcca tcgagcggga ggcccggggc gggaatccgt cggccatccc
      241 gttcccccgg gccgtcttca gcgacggaag cctggacttc agcttcagcg gcctcaagac
      301 cgccgtggcg cactacctga agggtctgga gcggaatcgc ccgggacccg gcaagcctgc
      361 gctcgatccc caggcggtgc gagatgtgtg tgccagcttt caacaggccg ccgtggacat
      421 gctgacggaa ggggtgatgc gggcgatccg ggatctgggc gtggggcaac tcgtggtggc
      481 aggcggggtc gcatgcaaca cggcgctgcg ccaggccctc aaggaagagg cggaggccga
      541 gggaatccag ctctgcgtgc cctccccggc gctctgcacg gacaacgccg ccatgatcgc
      601 cgtcgccggg cacatccgcc tgcggcgtgg cgaacgagcg ggctgggacc tcaacgccgt
      661 ggcgaacctc cccctggcct gaggaaagct agggtgggct cttcggcgat ggagctatag
      721 gagttgctga cgtgtcgata tccacgcaca cccgacccat cttgacctat ggccaaccca
      781 ttttgcggca agcggctcgc cccgtccgca gcatcgacgg ggagctgcag cagctcatcg
      841 acgacatggt ggagacgatg tatgccgccc ctggcgtggg cctggccgcc aaccaggtgg
      901 gaagcctgca gcgggtcttc gtgacaaacc cttccgaaga gcacgatccg gggaagctcc
      961 tagtcctggt caaccccgaa ctggtggaga gcgagggaga ggtcgtcgcc gaggaaggct
     1021 gtctgtccat tccggacttc cgcgaggagg tccggcgcgc ccgtcgcgtg ctgctccgcg
     1081 gtctggatcg aaatgagcgg cccgtggaaa tcgaggccca ggatctgcta gcccgcatct
     1141 tccagcacga gctggatcat ctaaacggcg ttctcttcgt ggaccggctg agccccgcca
     1201 agcgcgacat cctgctccga aagctcagga aaaccttcct ggagcgctaa gccccggcgc
     1261 cggcaggacg cctcctccgt gagaacggcg gccacgcctt ctgcggttac ttcgtctttt
     1321 gcagttcggt cttgagggcc ccggcctcca tgatcacctg gatgaaggcc ttggcccgga
     1381 ggcctgccag ccattccttg cgcttggcct cgaacttctc ctggaacagc tcatcccgaa
     1441 tcgtgtcccg gacctcggca aagggggcca cggtctcggt gcggacgcgc tccacctgga
     1501 tgaggttcca gccggcggag gtccggatga ggtcactcac cccacccacc ggcaagctga
     1561 aggccgccgc ctcgatttcg ggcgccaggt cgcccttgtg gatcgttccc aactcgccgc
     1621 ccttctccct ggtggggctg tcagaatact gctccgccat cttggcgaag tccgccccgg
     1681 aacggacctt gcccaggatc tcctcggccc gggccttggc ggcggtctcg tccgctcccg
     1741 gcggcaccga gatgagaatg tggcggatct ccgcatcagg cgtgcgctga tacttgtcct
     1801 ggtggtcctg gtaatactgc gtgagatcct cttcccggat gatgatcgag gatcggatct
     1861 ggcgggacag cacgcgactg atggcgagtc tctcctcgac gcttcgacga acctgctcga
     1921 acgtcatgcc ctcggcccgg aggagggccc ggaattgggc atcgtcggag acgttgttgt
     1981 tcttcttcag ctcttcgatg gcgttcgtga cgtcggcggc ggaaggcaac atcccctcct
     2041 tcttcgccac ctggtaggcg agccgacggt cgatgagttc atccagcagg cgcttctccg
     2101 cctcctccac gcgacggtcc cgctcgggac cgacgaagtc ccggaagatc ttccggatca
     2161 ccggctggcc ctcttcctgg acctcgctca gcgtcagggc ctcatcgttg accgccgcca
     2221 cgacttggtc gagtatcatg cccttgggct gggcctcggg gatggccgtc tgcgccagga
     2281 ccggtgcgac cgtgaggggc gcgaccagcc cgagcgcgac gacccagatg gttccggtca
     2341 cgacttgtct ccagtcttgg cacccccagt cccggggctt ggagcgcccg gcgggggacc
     2401 caggggcgtg ccgaccggga gcaacgattc gtcgcgggtg atcttggccg cgccctccag
     2461 atctttaatc cactgcgtga agcgctgatt ctggctctcg tccagcaggc gccgctgtag
     2521 ctgggccttg acctgttcga aggtgagggg ctgtcccttc tgccgatcca gcacctggat
     2581 gacgtgatag ccgaaggggg acttcacgac accgctcacc tcccccggct tcagggcaaa
     2641 ggcggccttg gcgaaatcct ggaccatctg atcccgcgag aagaaaccca ggtcgccccc
     2701 cttggccgcc gtgctcgtat ccttggac
//
LOCUS       DBNGZC010000587         1073 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1224898, whole genome shotgun sequence.
ACCESSION   DBNGZC010000587 DBNGZC010000000
VERSION     DBNGZC010000587.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1224898"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..1073
                     /locus_tag="AC16G0_10250"
                     /pseudo
     CDS             217..1073
                     /locus_tag="AC16G0_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070789.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT          206 a          323 c          361 g          183 t
ORIGIN      
        1 gaacaagaga gaggttgatt gccttatatc gcgtgattta tggaatagta atccgacaat
       61 tttagggaaa ccagcaaagt ggtgcattcg gcaatggctc atgatgggtt tcggggtggt
      121 cgttccaagc tgagcgtctg gcggatacca tcgccggaga gctgaccact tccgccgttg
      181 acgggcctgc ggtgcggcgc atcacggagg ggacccatgc tggtgcagat cgccaacctc
      241 atcgacgacg cgaagtgctt cgagaccgtg cgccagctgc gatggcccga gggcgtgaca
      301 tgcccgcact gccaatcacg caatgtcacc aaggacggcc gcgacgacac ccaggtagag
      361 cggcagcgtt atctctgcca cagctgccat cgccgcttcg atgacctgac cgggacgatc
      421 ttcgccggcc atcaccagcc cctgacgacc tggatcttgt gcctgtactt catggggctg
      481 aacctgtcca accgccagat cgccagggag ttggacttgg accgcagcac agtccaggag
      541 atgaccaccc aactccgaga cgggatcgtc cagcggcgcc ccgcggtgac cctctcgggg
      601 gaggtcgaat gcgacgaggt ctacgtggtg gccggccaca agggccatcc cgaggcggtg
      661 ctcaaaaagg gcgcgagggg cgacgccggc gcctgaaggg cgagcccggt cgcggcacgc
      721 tggagaagga gaagccgccg atcctgggcc tgctccagcg gaccggcgag gttgtcatca
      781 cgatgctggc gaatgtccag caggccacga tcgcgccgct gattcggcag ttcatcgcgc
      841 ccgggagcac ggtcttcacc gacgagtatg acatctacca tcgcctgacg gagtgggggt
      901 acagccaccg gacggtgtgc catgccgcgg gggagtacgc tcgggacgag gatggcgatg
      961 gtcgttgcga ggtccacgtc aacacgatcg aggggttctg gtcgctgctg cgcagttggc
     1021 tgcgcccgca tcggggcatc tcgcaggaac agttgcccgt gtacctgggc ttc
//
LOCUS       DBNGZC010000588         2068 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1122726, whole genome shotgun sequence.
ACCESSION   DBNGZC010000588 DBNGZC010000000
VERSION     DBNGZC010000588.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1122726"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="AC16G0_10255"
     CDS             <1..981
                     /locus_tag="AC16G0_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR5100031.1"
                     /translation="TGVMVSPLCLGAMNFGGPTTEEDSFIILNRALEAGVNFIDTANV
                     YTGGASERIVGKFLKDGGKRDQVVLATKVYSRVGDLPNDAGASRFHVIKACEDTLRRL
                     QTDRIDLYQQHRPSLTIPQDETLRAYDDLVRSGKVRYIGSSTFPAWMVMEALAISDRY
                     GLARFISEQSPYNLLDRRVENELIPLCLKHGLAMLPWSPLAGGILAGRYSASETFPEG
                     SRATRMGAMFTERITGRGKKVAVEVAKMARNRGMTASQLCLLWLKDQPGVTSPITGPR
                     TLAHLEDALAILGKKLDDADRPVFDALVHPGNAVADFFNSNEWMKARIKE"
     gene            1008..2039
                     /locus_tag="AC16G0_10260"
     CDS             1008..2039
                     /locus_tag="AC16G0_10260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR5100032.1"
                     /translation="MAFEKLAVLGVGAIGSTLGAYLTRAGCDITLIDLWPAHVEEMKQ
                     RGLRVTAQDEEFTVRVKAVHLADVCSLHEHFDAVLLSVKSYDSVWAVRFIEPYLRPAG
                     ILVSAQNGINEDWVAPLIGYTRTLGCVVTLSAGLYEPGHVTRTSLPSRPSFALGELNG
                     MITPRVQELVKLLSPVGPAHVTTNLWGDRWAKLTINCMANALAGITGMAVEPLRANPD
                     VFSVAVRVAGEALTVAQRLGVQVEPLGGIPAQAYLDAACGVGLEELHHKRVEAGRAIG
                     AGRPSLFQDILKGRRTEVDYLNGYVVRKGREVDVPTPMNQTIVEVTQRLEAGDLKPDP
                     ANLTLLTKH"
BASE COUNT          385 a          681 c          667 g          335 t
ORIGIN      
        1 accggcgtca tggtcagccc gttgtgcctg ggagcgatga acttcggcgg tcccaccacc
       61 gaggaagatt ctttcatcat tcttaaccgc gccctggagg caggcgtcaa cttcatcgat
      121 accgccaacg tgtacaccgg tggcgccagc gagcgcatcg tcggcaagtt cctgaaggac
      181 ggcggcaagc gcgaccaggt ggtgctggcg acgaaggtct acagccgggt gggcgatctg
      241 cccaacgacg ccggcgcctc ccgttttcac gtcatcaaag cctgtgagga tacgttgcgt
      301 cgcctgcaga ccgaccgcat cgacctgtac cagcagcacc gtccatccct caccatcccc
      361 caggatgaga cgctccgggc ctacgacgac ttggtgcgct cgggcaaagt gcgttacatc
      421 ggcagcagca ctttcccggc ctggatggtg atggaggcgc tggcaatcag cgaccggtac
      481 gggctggcgc gattcatcag cgaacagtcg ccctacaacc tcctcgaccg gcgagtggaa
      541 aacgagctga ttccgctgtg cctgaaacac ggtctggcca tgctgccctg gtcgccgctg
      601 gcgggaggca tcctggccgg ccgatattcc gcgtccgaga cattccccga gggctcacgc
      661 gccacccgca tgggggcgat gttcaccgaa cggatcacgg gccgggggaa aaaggtcgcc
      721 gtcgaggtgg caaagatggc ccggaaccgc gggatgaccg ccagccagct ttgcctgctc
      781 tggctcaaag accaaccggg ggtcacctcg cccatcaccg gaccgcgcac cctggcccac
      841 ctggaggacg ctctggccat tctggggaag aagctggacg acgccgaccg gccagtgttc
      901 gacgccctgg tgcatccggg caacgccgtg gccgactttt tcaacagcaa cgagtggatg
      961 aaagcccgta tcaaagaatg accggcgtcc tggagaggag ggagcccgtg gcattcgaga
     1021 agttggccgt actgggtgta ggggccattg ggagcaccct cggtgcctat ctcacccgcg
     1081 caggatgcga catcaccctg atcgatctgt ggcccgccca cgtcgaggag atgaagcagc
     1141 gtggcctgag ggtcacggcg caggacgagg agttcacggt ccgggtcaag gccgtccacc
     1201 tggcggacgt gtgcagcctc cacgagcact ttgatgcggt cctgctgtcg gtgaagtctt
     1261 atgacagcgt gtgggcggtg agattcatcg agccgtacct cagacccgca ggcatcctcg
     1321 tctcggcaca gaacggcatc aacgaagact gggtggcccc cctgatcggc tacacacgga
     1381 cgctgggctg cgtggtgacc ttgagcgcgg gcctctacga gccggggcac gtcacccgca
     1441 ccagcttgcc ctcgcggccc tccttcgccc tgggcgagtt gaacggcatg atcaccccca
     1501 gagttcagga gctcgtcaag ctgctctctc ccgtcggccc ggcccacgtc acgaccaacc
     1561 tctggggtga ccgctgggcc aagctcacca tcaactgcat ggccaacgcc ctggcgggca
     1621 tcaccgggat ggccgtggag cctctgcgcg ccaaccccga cgtcttctcc gtagccgtca
     1681 gggtcgccgg cgaggcgctg acagtggccc agaggctggg cgtgcaggtg gagcctttgg
     1741 gcggcatccc cgcgcaggca tacctggatg cggcctgcgg cgtgggcctg gaggagcttc
     1801 accacaagcg ggtggaggcc ggtcgcgcca tcggggccgg gcgaccctct ctcttccagg
     1861 acatcctgaa gggccgccga accgaggtgg actacctgaa cggctacgtg gttcggaagg
     1921 gacgagaggt ggatgttccc acccccatga accagaccat cgtcgaggtc acccagcgac
     1981 tcgaggcggg ggacctgaag ccggaccccg ccaacctcac gctgctgacc aaacactaat
     2041 aaagccttgc aggctgctga aaaaggcc
//
LOCUS       DBNGZC010000589         4123 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_637227, whole genome shotgun sequence.
ACCESSION   DBNGZC010000589 DBNGZC010000000
VERSION     DBNGZC010000589.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_637227"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(392..703)
                     /locus_tag="AC16G0_10265"
     CDS             complement(392..703)
                     /locus_tag="AC16G0_10265"
                     /inference="COORDINATES: protein motif:HMM:NF018058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HIR5100033.1"
                     /translation="MRQFYFTLGLILGLAVAIFAMQNTTAVEVRFLGWMANGPLALVV
                     LASAAAGVLMALLFCLPAMLRGRWRVRSLERRLGPGGGPATSASGGDLDAKTPDKTVP
                     R"
     gene            complement(763..2229)
                     /locus_tag="AC16G0_10270"
     CDS             complement(763..2229)
                     /locus_tag="AC16G0_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HIR5100034.1"
                     /translation="MRSHWALYPCAVVALGLLMWVVSPVSAQQSSGQQTSGHEAEQKD
                     MRLVGFNDLQARSAYQPIIQKQGDRWIAYVGHHGGEASNPLSGKVEPNGTSIVDVTNP
                     AQPRYLYHIPGGKGAGEAGGAQMVRACEGKSLPKGTPGKTYLLRTNGNLAHEIWDVTD
                     PANPTFVVTVVTGLNGTHKNWWECDTGIAYLVSDGRPFGWRSNRMTKIYDLSDPARPR
                     FVRDFGLPGQEPGSSGPVPGGVHGPIRLGNRVYFAYGTSADGVLQIVDREKLLRGDPT
                     SSSPLAPTPANLLYPQVGRLDFSPNWGGHTSFPILGVEIPDFAKNLSGRVRDFVLMTS
                     EATGNSCQEFRHLTFLVDITTESKPFAVANFQVPESSGNFCDRGGRFGPHASSESFTP
                     IYYKKLVFLSYFNAGVRAVDLRDPYRPTEVAFYIPATTANTDKRCARIGGAETCKVAF
                     QTNNVEVDERGFIYIVDRANTGLHIVELTGSARAIIER"
     gene            complement(2343..3614)
                     /locus_tag="AC16G0_10275"
     CDS             complement(2343..3614)
                     /locus_tag="AC16G0_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR5100035.1"
                     /translation="MVWLIPLLFVFFFIGMPITFSLGVTSLLGLMLTKVPLLLIVQRL
                     WTAVDTFSLVAVPLFILAGDLMSRGGISRRLVDFAQALVGHFTSGLAMVAVVACMFFA
                     AVSGSAIADAAAIGGILIPTMIANGYSPPFTASLVAAAGTIGPIIPPSIPMVLYGAMT
                     NTSIAMLFAGGFLPGVLMGVGLMIYSYYVGKQRGYLGREKHAGLKEVAQGFVNALLAM
                     LMPIIIIGGIISGVFTPTESGVVAVVYALLLGKFVYRELTWGDVTRLLYECGLLTGKI
                     LFILGTASIFSWLLTVEGIPQQVTAYIASLNAGWVMMLLLINLILLFVGTFIDTISAL
                     VIFTPLLLPLAMSVGVDPIHFGIILAVNLTIGMITPPVGVVLFVTTAIAKLTIREMMR
                     DLTPMIGVLIVALAITTYWRAMVMWIPNLLR"
     gene            complement(3614..>4123)
                     /locus_tag="AC16G0_10280"
     CDS             complement(3614..>4123)
                     /locus_tag="AC16G0_10280"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR5100036.1"
                     /translation="PPPPPPPPAYQGEVFVLKILDRLLKYLLTFLIGLLTVSVFLQVL
                     IRFVFKYPLPWTEEVSRIAFVYSIFVGAAIAVREKTHLNVDFLLVVLPAGLARALKLV
                     GTVLVGVFLVFMTWQGIVFVRATGVQVTPVMQVPFRYLYLIIPSSGGLMLLYLILGSI
                     DDIRQGAKR"
BASE COUNT          830 a         1263 c         1334 g          696 t
ORIGIN      
        1 gatttgggtg cggatcgctg tggtctctcg ttgggccatg gagtgggtaa tgaggaagat
       61 ggccacgttg acggcgatca ggctgatggt cacccagggc agacgcgtga cccgctggtc
      121 ctcgtgactg atggggatca ggaacattgc cctgcctctg gctgcggtcc ggcctgcaac
      181 attggtggcg ccttcctctc cttcaggcga cccccggtag ctccagtgga ttgtcgccaa
      241 aacgtgccga cgctaccagc atcggcgtca gagtcaaggt gaaagtggct taggtacggc
      301 ggtgcagtgc agggccggag ggtagcaggc gtgccaggga ggcggcggcg cagtgagggc
      361 aggggtgcct gtgccggcat cgtgtgcagg actatcgcgg aaccgtcttg tccggtgtct
      421 tggcatccag gtcccccccc gaggccgaag tggctggacc gccgcccggc ccgagacggc
      481 gctccaagct ccggacgcgc cagcggcccc tcaacattgc cggaaggcag aagagcagcg
      541 ccatcaagac gccagcggcc gccgaggcca gtacgaccag cgccagcggc ccgttcgcca
      601 tccaccccag gaaccgcacc tcaaccgccg tcgtgttctg catcgcgaag attgctacgg
      661 ccaatcccag gatcaagccc agcgtgaagt aaaactgccg catgcgatcg cctcatctct
      721 cctacaggtt gccgctcccg ggtgcaccgg agctacctca gctcacctct cgatgatcgc
      781 tcgcgctgaa ccggtgagct caacgatgtg caagccggtg tttgcccgat ccacgatgta
      841 gatgaacccg cgctcgtcca cctcgacgtt gttggtctgg aaggcgacct tgcacgtctc
      901 cgccccgccg atccgtgcgc agcgcttgtc ggtgttcgcc gtggttgccg gaatgtaaaa
      961 ggcaacttcc gtcggccgat agggatcgcg gaggtccacc gcccgaaccc cggcgttgaa
     1021 gtaggacagg aatacgagtt tcttgtagta gatcggagta aaagactcgc tgctggcgtg
     1081 gggaccgaat cgcccgcccc ggtcgcagaa gttcccgctg gattccggga cctggaagtt
     1141 ggcgactgcg aacggtttgg attcggttgt gatgtccacg aggaacgtca aatgccggaa
     1201 ttcctggcag ctattcccgg tagcctccga agtcatgagg acgaagtcgc gaactcgccc
     1261 gctgaggttc ttggcgaagt ccggaatctc gacgccgaga atggggaagc tggtatggcc
     1321 cccccaattg ggagagaaat cgagccgacc gacttgcgga tagaggagat ttgccggcgt
     1381 gggagccagg ggactggacg aggtcggatc gccccgcagc aatttttccc ggtccacgat
     1441 ctggagcacc ccgtccgcac tcgtcccgta ggcgaagtag acgcgattcc cgagccggat
     1501 cggaccgtga actcccccag gcaccgggcc ggaggatccc ggctcctggc ctggaagtcc
     1561 gaaatctcga acaaaacgag ggcgcgcagg gtcgctcagg tcatagatct tcgtcatccg
     1621 gttggatcgc cacccgaacg ggcgtccatc ggacacgaga taggcgattc ccgtgtcgca
     1681 ttcccaccag ttcttatgcg tgccattgag cccggtaacc acagtcacca cgaacgtggg
     1741 gttggccggg tccgtcacat cccagatttc gtgcgccagg ttcccgttgg tccggagcag
     1801 gtacgtcttt ccgggagtcc ctttcggaag actcttcccc tcgcaggctc gaaccatctg
     1861 ggcaccaccg gcctcccccg ctcccttgcc acccgggatg tggtagagat atcggggctg
     1921 agccggattc gtcacatcca cgatggacgt accgttgggc tcgaccttgc cgctgagcgg
     1981 attggaagcc tcccccccat gatggccgac gtacgcgatc caccggtccc cctgcttctg
     2041 aatgatgggc tggtaggccg agcgggcctg gaggtcattg aagcccacca gtcgcatatc
     2101 cttctgttcc gcctcgtgcc ccgacgtttg ctgcccgctc gattgctggg ctgagaccgg
     2161 gctgacgacc cacatcagga ggccaagggc aacgacggca catgggtaga gggcccaatg
     2221 gcttcgcatc accgctactc cttgcttgaa acggttgcaa cccgcgcccg gtcgatggga
     2281 ggtcgcgtct ttcccggctc ttcactgcag gctttctcgt cttgcgcgcc gatgatagcc
     2341 cctcacctca gcaagttcgg gatccacatg accatcgcgc gccagtaggt ggtgatggcc
     2401 agcgccacga tcaagacgcc gatcatgggc gtgagatccc gcatcatttc acggatggtc
     2461 agcttggcga tggcggtggt gacgaacagc accacgccca ccggcggggt gatcatgccg
     2521 atggtcaggt tgacggcgag gatgatgccg aaatggatcg gatccacgcc caccgacatg
     2581 gccagaggga gcaacagggg ggtgaagatc accagggcgc ttatggtgtc gatgaacgtc
     2641 cccacgaaga gcaggatgag gttgatcagc aggagcatca tgacccatcc ggcattcagg
     2701 ctcgcgatgt atgcggtgac ctgctggggg atcccctcga ctgtgagaag ccacgagaag
     2761 atagaggccg tgcccaggat gaacaagatc ttgccggtga ggaggccgca ctcgtagagg
     2821 agccgtgtga cgtcccccca tgtcagctcg cggtacacaa actttcccag gagcagcgca
     2881 tagaccaccg ccaccacccc ggattcggtg ggggtgaaga ccccgctgat gatcccgccg
     2941 atgatgatga tcggcatgag catggccaaa agcgcattca cgaagccctg ggcgacctcc
     3001 ttcagccctg cgtgcttctc acggcccagg tagccgcgct gctttcccac atagtaagag
     3061 tagatcatga gccccacgcc catcagcacc ccggggagga aacccccggc gaagagcatg
     3121 gcaatggacg tgttggtcat ggccccgtag agcaccatgg ggatgctggg cgggatgata
     3181 gggccgatgg tgccggctgc ggccaccagg gaagcggtga agggcgggga gtacccattg
     3241 gcgatcatgg tcgggatcag gatgccgccg atggctgcgg cgtcggcgat ggccgagccg
     3301 gaaacggccg cgaaaaacat gcaggccacc acggcgacca tggccagtcc cgacgtgaaa
     3361 tggcccacca aggcctgggc gaagtccacg agtcttcggg aaatcccgcc ccgagacatc
     3421 aggtcgccag ccaggatgaa gagcggcacc gccaccaggg agaaagtgtc caccgccgtc
     3481 cagaggcgct gcacgatcag gagcagcggc accttggtga gcatcaggcc gagaagcgag
     3541 gtcactccca ggctgaaggt gatgggcatg ccgatgaaga agaatacgaa gagaagcggg
     3601 atcagccaga ccatcagcgc ttggccccct ggcggatatc gtcgatgctt ccaaggatta
     3661 ggtagaggag catcagcccg ccgctgctcg ggatgatgag gtacaggtag cggaacggga
     3721 cctgcatcac gggcgtcacc tgaacacctg tggcccggac gaacacaatc ccctgccagg
     3781 tcatgaacac caggaagacc cccaccagga ccgtgcccac cagcttgaga gcacgcgcca
     3841 ggcctgcggg caggaccacc aggaggaagt ccacgttcag gtgggtcttc tcccggacgg
     3901 cgatggccgc cccgacgaat atcgaataga cgaaggcgat tcgcgagact tcctccgtcc
     3961 agggcagggg gtacttgaag acgaagcgga tcagcacctg caggaacacg ctgaccgtga
     4021 ggaggccgat caggaaggtc aggagatact tcagcagccg gtcgagaatc ttgagcacaa
     4081 acacttcccc ctggtacgca ggcggggggg gggggggggg ggg
//
LOCUS       DBNGZC010000590         2838 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2398994, whole genome shotgun sequence.
ACCESSION   DBNGZC010000590 DBNGZC010000000
VERSION     DBNGZC010000590.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2398994"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="AC16G0_10285"
     CDS             complement(<1..939)
                     /locus_tag="AC16G0_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5100037.1"
                     /translation="MKMRIAVIATLALCIAAPVFGAEPIKIGAFFALSGPAANIGTPT
                     KLVAQMVVDKINKEGGINGRALELILGDTESDPTKAAVIAKKFIFSDKVAAIIGPTST
                     AEGMSVKKIVEEAGVPTFMTVGGDPVIMGGNLGAFTYVFKSPQRSSTAVKKLYGYLKA
                     KGLTKAALFTASDSFGKDGLGWLEKLAPDFGISFTAKESFGPSDTDMTAQLTKIKNSA
                     PQAIVTWTIGPVGSIVAKNKAQLGISTPLFQCHGLPDPKYIELAGKAAEGDRMPATKL
                     MVADSLPNSDPQKKVIVEFIHLYRDVYKHDTEFPINT"
     gene            complement(970..2277)
                     /locus_tag="AC16G0_10290"
     CDS             complement(970..2277)
                     /locus_tag="AC16G0_10290"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIR5100038.1"
                     /translation="MSRLTFLPPFTANDELLAHQLKGLQWTVRHAREGSSFYRALLDK
                     AGVGPEDIRSLDDLARLPLITADDLRDDYPFPLRSVPFEQVVRIHSSSGTTGKRKILC
                     YTQKDVDDWAHFFARCYEMAGVTPLDRVQIAVGYGLWTAGVGFQAGCERVGAMAVPTG
                     PGNLDLQCEFLMDLQSTVFCCTASMGLLLAEEVNKRGLAEKISLRKVIYGSERSSRSM
                     RKKISELLGGVELFDIPGLTELYGPGTGIECPHHDCIHYWGDYYLLEILDPETLAPVA
                     PGEWGEMVVTTLAKEAAPLIRYRTRDITRIIPGPCTCGSILPRHSRIRGRTDDVFKFR
                     GVNIYPSTIDTILSSIPGLGSEYQVHLSRDAEQRDHLRLVVERGDGVERGRVEELARE
                     IVHSVKHKVMVTAQVEVVDYGSLPRSERKTKRVFDTRIEDSIV"
     gene            complement(2581..2799)
                     /locus_tag="AC16G0_10295"
                     /pseudo
     CDS             complement(2581..2799)
                     /locus_tag="AC16G0_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          466 a          913 c          958 g          501 t
ORIGIN      
        1 ggtgttgatg ggaaactccg tgtcgtgctt atagacgtcc cgatagagat ggatgaactc
       61 gacgatgacc tttttctgag ggtccgagtt cgggagggag tcggcgacca tgagcttggt
      121 ggccggcatg cggtcaccct cggcggcctt gcccgccagc tcgatgtact tcggatcggg
      181 aagcccgtgg cactggaaga ggggggtgga gatcccgagc tgtgccttgt tcttggccac
      241 gatggaaccc accgggccga tggtccaggt gacgatggcc tggggcgcgg agttcttgat
      301 cttggtgagc tgagccgtca tgtccgtgtc cgaggggccg aaggattcct tggccgtgaa
      361 gctgatgccg aaatccgggg caagcttctc cagccagccg agcccatcct ttccgaagga
      421 gtccgacgcg gtgaagaggg ccgccttggt cagccccttg gccttgagat agccatagag
      481 cttcttcacg gccgtggacg agcgttgcgg ggacttgaag acgtaggtga aggcacccaa
      541 gttgccgccc atgatcaccg ggtcgccccc cacggtcatg aaggtgggga ctcccgcctc
      601 ctccacgatc ttcttgacgc tcatcccctc ggcggttgag gttgggccga tgatggcggc
      661 caccttgtcg ctgaagatga acttcttcgc gatgacggcc gccttggtcg ggtcgctctc
      721 ggtgtcgccc aggatcagct ccagggcgcg cccgttgatc cctccctcct tgttgatctt
      781 gtccacgacc atctgggcca cgagcttggt cggcgtgccg atgttggcgg cggggccgga
      841 cagggcgaag aaggcaccga tcttgatcgg ctccgcgcca aagacagggg cggcaatgca
      901 cagggccagc gtggcgatga cggcgattcg catcttcatg accgttgtcc cccctcagta
      961 atgcgttgct tagacgatgg agtcctcgat ccgcgtatcg aacacccgct tggtcttacg
     1021 ctcggagcgg gggagtgacc cgtagtccac cacctcgacc tgggccgtca ccatgacctt
     1081 gtgcttgacc gagtggacga tctctcgcgc cagctcctcg accctccccc gctccacccc
     1141 atcccctcgc tccaccacca ggcgcaggtg gtcgcgctgc tcggcgtcac gggaaaggtg
     1201 cacctgatac tccgaaccca agccgggaat ggacgagaga atggtgtcga tggtggaagg
     1261 gtagatgttc accccccgga acttgaacac gtcgtcggtg cgcccccgga tccgcgaatg
     1321 gcgcggcagg atggacccgc aggtgcaggg ccccggaatg atccgggtga tatcccgcgt
     1381 ccgatagcgg atgagggggg ccgcctcttt cgccagggtg gtgaccacca tctcacccca
     1441 ctcgcccgga gcgaccggcg cgagggtctc gggatcgagg atctccagga ggtagtagtc
     1501 gccccagtag tggatgcagt cgtggtgcgg gcactcgatc ccggtgcccg ggccgtacag
     1561 ctcggtgagc cccgggatgt cgaacagctc caccccgccg agcagttcgg agatcttctt
     1621 ccgcatggac cgcgacgacc gctccgagcc gtagatgacc ttccggaggc tgatcttctc
     1681 ggccaggccc cgcttgttga cctcctccgc cagcagcaac cccatggagg cggtgcagca
     1741 gaagacggtg gactgcaggt ccatcaggaa ctcgcactgg aggtccaggt tgcccggccc
     1801 ggtgggcacg gccatggccc cgaccctttc acagcctgcc tggaacccca cccccgccgt
     1861 ccagaggccg tatcccacgg cgatttgcac gcggtccagg ggcgtcacgc cggccatctc
     1921 gtagcagcgg gcaaagaagt gggcccaatc gtccacgtcc ttctgggtgt agcagaggat
     1981 cttccgcttg ccggtcgtgc ccgacgacga gtggatgcgc acgacctgct cgaagggaac
     2041 cgaccggagc gggaaggggt agtcgtcccg cagatcgtcg gcggtgatga ggggaagcct
     2101 cgccaggtcg tccagggaac ggatgtcctc cggcccgacc ccggccttgt ccagcagggc
     2161 gcggtagaag gatgagccct cgcgtgcgtg ccgcacggtc cactgcaggc ccttgagctg
     2221 atgggccagg agttcgtcgt tggccgtgaa gggcgggagg aaggtcagtc ggctcatgtc
     2281 gggcttctcc agtctgacgt tggtgagacc cgcggccccc aggcggctcc gggccgggtt
     2341 atccttggag gccgcaccca ggaaggcgtg acgcagctcc gggtgaacca gcaactcgtc
     2401 actcctcccc gaccggacga ttcttccgtt ggccagcagg tgggcggtgg tggaggcggt
     2461 aagcgcccgg gccgcgttct gctccaccag gaggatcgtc gtccccgccc ggcccagccg
     2521 gtggatgatg tcgaagatct catcggtgat gaggggggcg agacccagac tcggctcgtc
     2581 aaggagcagg agccgcggtc gcgccatcag ggctcggccg atggccacca tctgctgctc
     2641 gccccccgag agggtggcgg tcggctggtc ggcgcgatcc ctgagacgcg ggaaccgttc
     2701 gaagacctcc tccacccggc gggacagctc cgctttcttg ggcttgccgg ccagggcccc
     2761 caggacaagg atttcccgga cgctcagctc gccgaacatc tcgcgtccct cgggggagag
     2821 gaccacaccc agggcggc
//
LOCUS       DBNGZC010000591         2153 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1713210, whole genome shotgun sequence.
ACCESSION   DBNGZC010000591 DBNGZC010000000
VERSION     DBNGZC010000591.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1713210"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..513
                     /locus_tag="AC16G0_10300"
     CDS             268..513
                     /locus_tag="AC16G0_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100039.1"
                     /translation="MKKLAVFALVAGLVVALGAPAFAFKCPTLIKQANEEMAKMDQNS
                     DKVKKAKELIEEADKLHKAGNHADSVKKAEEALADLK"
     gene            772..>2153
                     /gene="glgP"
                     /locus_tag="AC16G0_10305"
     CDS             772..>2153
                     /gene="glgP"
                     /locus_tag="AC16G0_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HIR5100040.1"
                     /translation="MIPAHKYTVTPQIPGRLKDLQRIAMNLWWTWDPEAIDLFRRLEP
                     NRLLWERCYGNPIRMLGLVSQERLMELTTDEGFLAHLDRVSTRLSSYMEHPTWFAQTH
                     SPSNLCVAYFCAEFGIVEGLRTYSGGLGVLAGDHLKSASDLGIPLVAVGLLYRRGYFR
                     QYLNADGWQQEQYPEADYYTLPLGLERLTDGNPLTVELDFPGRKVRAQVWRAQVGRVA
                     LYLLDSDLDANAAEDRAITGYLYGGDRDTRIRQEILLGIGGLRALAAMGIEPTVCHMN
                     EGHAAFLALERIRQAMQRHRVSFAEARELVAASSVFTTHTPVPAGIDVFPPDLMDRYF
                     GACFAQLGLSRDAFLALGRQNPSDGNEPFSMAVLAIRLSSATNGVSALHGRVSRRMWA
                     GLWPGVPLDDLPITSITNGVHVRGWLSHDMAGLFDAYLGPNWISHPADQSVWERVEQI
                     PDAELWRTHER"
BASE COUNT          389 a          674 c          704 g          386 t
ORIGIN      
        1 ttgttagggt attctcgttc caaggccaag gcaagttgaa agatgcaggt gtcgggaaca
       61 cctgacgacc ccctgcgatc cgaggcgtga cacgcctacc ctgcccccat gaccccggga
      121 cccgtcgggt gaaaaatttg cccaggcggg gaaccgtttg cgacccgggg ttgttcttct
      181 tgataacccc ggcacctggc cccaggggtt acattgtccc aacaacgcaa gaaaatggct
      241 tgcaaccctg aaacaaggag gcacgtcgtg aagaaactcg cagtctttgc gctggtggct
      301 ggcctggtcg tggccttggg ggctccggcc tttgccttca agtgcccaac cctcatcaag
      361 caggcgaacg aagagatggc aaagatggac cagaacagcg acaaggtgaa gaaagccaag
      421 gaattgatcg aggaggcgga caagctgcac aaggccggca atcacgccga ttccgtgaag
      481 aaggccgagg aggccttggc cgatctgaag tagacggtaa gccgctggat cgcgataccg
      541 acgggccatc cggggtaatc ccggatggcc cgtggtattt gaggccgcca gccgcgaggg
      601 gatccggatc cggtggcggg gcggagatct gcttgctctg gccagcgggt cgcagtatgc
      661 tctcgacatc cagtcgagac aacctgccct ggcccggctt tcgcccgatc tgtcccgcga
      721 ctgtctccgc tcccccgacc gggggaggag cctggcgcaa aggaggctga cgtgatccca
      781 gcccacaagt acaccgtcac cccccaaatc cccggccggc tcaaagacct ccaacgcatc
      841 gcaatgaact tatggtggac ctgggatccc gaggcaatcg acctgttccg gcgcctggag
      901 cccaaccgtc ttctctggga gcgctgttac ggcaatccca tccggatgct gggcttggtc
      961 tcccaggagc ggctgatgga gctgacgacc gatgagggat tcctggcgca cctggaccgc
     1021 gtgagcacca gactgtcaag ctacatggag catcccacct ggtttgccca gacccacagt
     1081 ccttccaacc tgtgcgtggc ctacttttgc gcagagttcg ggatcgtcga aggcctgcgg
     1141 acgtattcgg gcggcctggg ggttctggcg ggggatcatc tcaagtcggc cagcgatttg
     1201 gggatcccgc tggtggcggt ggggctcctg taccggcgcg gatacttccg ccagtacctg
     1261 aatgccgatg gctggcaaca ggagcagtat cccgaggccg attactacac cttgccgctg
     1321 ggcctggagc ggctgacgga cggcaatccg ctgaccgtcg agttggattt ccccgggcga
     1381 aaggtgcggg cacaggtctg gcgggcgcag gtgggccggg tggccctgta cctcctggac
     1441 tcggacctgg acgccaatgc cgccgaggat cgcgccatca ccggatacct ctatgggggc
     1501 gatcgcgaca cgcgcattcg ccaagagatc ctgctgggca tcggagggct gcgtgccttg
     1561 gcggccatgg ggatcgaacc cacggtttgc cacatgaacg agggccacgc ggcattcctg
     1621 gcgctcgagc gcatccggca ggcgatgcag cggcaccggg tgagcttcgc ggaggcacgg
     1681 gagctggtgg cggccagcag cgtcttcacc acccacaccc ccgtcccggc cggaatcgac
     1741 gtcttcccgc cggacctgat ggatcgctac ttcggagcct gcttcgcaca gctcgggctc
     1801 agtcgggatg cttttctggc tttggggcgg cagaatcctt cggatgggaa tgaaccgttc
     1861 tcgatggcgg tcctggccat ccgactgtct tcggcgacca acggggtgag cgccctgcac
     1921 ggtcgggtct cgcgccggat gtgggctggc ctgtggcccg gggttccgct ggacgatctg
     1981 ccgatcacgt ccatcaccaa cggcgtccat gtccggggat ggctctccca cgacatggcc
     2041 ggcctcttcg atgcctacct tggcccgaac tggatctccc atcccgccga tcagtcggtg
     2101 tgggagcggg tcgagcagat tccggatgcc gaactctggc ggacccacga gcg
//
LOCUS       DBNGZC010000592         4059 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1122812, whole genome shotgun sequence.
ACCESSION   DBNGZC010000592 DBNGZC010000000
VERSION     DBNGZC010000592.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1122812"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="AC16G0_10310"
     CDS             complement(<1..951)
                     /locus_tag="AC16G0_10310"
                     /inference="COORDINATES: protein motif:HMM:NF016236.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0120010 - intermembrane phospholipid
                     transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HIR5100041.1"
                     /translation="MRIVKKIGGSMWWMAVLVTTLVSIGPGALSFAGAEEGLDPAVNV
                     SSGPSGLPSAAAVPDEAPPTAPLGDAVPARDTAEATPGGPQEGWNPVEASSFLSESPR
                     DAPVSDEAPLAMPVSDVVPDEKTAEETRGAPQVEPAASEDYDPWEPFNERMFTFNREL
                     DRYVLKPVATGWDTVVPNAVQRGLGRMLDNVGMPRRFVNSLLQGKVEGAGQELARFLL
                     NSTFGLAGFFDVATEAGLQASDKDTGQTLGVWGVPPGPYLVLPFLPPMTVRDGIGSGI
                     DVLLNPVTYFAPFAALLGIKGADTMNSRSLNLEVFEHVEEG"
     gene            complement(1690..2019)
                     /locus_tag="AC16G0_10315"
     CDS             complement(1690..2019)
                     /locus_tag="AC16G0_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100042.1"
                     /translation="MSFHETEHGMTHEDIVPLVDRVASLLATERGLSRDRVATLAHKM
                     LHELAGDVRNDFNVFEWEGAPAEAAQRRIAYWTKEQHWQAIAPEIAQTLRAAKATRQE
                     IEVLVMG"
     gene            complement(2323..3147)
                     /locus_tag="AC16G0_10320"
     CDS             complement(2323..3147)
                     /locus_tag="AC16G0_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding periplasmic protein"
                     /protein_id="HIR5100043.1"
                     /translation="MHSKRLVAVRTLVTLLFGALTLLGATRGYAGTLEAVKARGILTV
                     GVRWDFPPLSYLDGQGQPTGFEVDLARYLARQLLGDEGKLRLVRVNAGDRLTAILSGS
                     ADMLIASVSATEDRAAVFALSEPYFLSGTLLLVPRDSSIRDIGDLPGKRVAVLEGTIQ
                     EGVWEPVVREPVVRGVIRVQFRNVAAAVAALRAGQVDALAEDDVLILALAKQYPDLVA
                     VGRPFRPQPYAIAMHKGERELRDWVNEQLRQAKTDGTLDTLWNRYFGDAATILLRP"
     gene            complement(3266..3511)
                     /locus_tag="AC16G0_10325"
     CDS             complement(3266..3511)
                     /locus_tag="AC16G0_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100044.1"
                     /translation="MNTGARFLTVVDAYPGYCQMCHEPRAVGRIVVAGWPYRGLACSR
                     CLSRLADEVQRRGSALPDGASEVAALVASVGGNGHLR"
     gene            complement(3732..>4059)
                     /locus_tag="AC16G0_10330"
     CDS             complement(3732..>4059)
                     /locus_tag="AC16G0_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100045.1"
                     /translation="KPVPVANLQENPDLPMLLLVNDTEESPPAILLGVDARNGKDTWS
                     LSTDPIILIVVFADAATVQDVYVDIGFVDEGKASGTYAAVDPANDTALPELLKAVPAT
                     GRRSNI"
BASE COUNT          687 a         1363 c         1268 g          741 t
ORIGIN      
        1 gccttcctcg acgtgctcga acacttccag gttgagggag cggctgttca tagtgtccgc
       61 ccccttgatg cccaaaagcg cggcgaacgg cgcaaagtag gtcaccgggt tgagcagcac
      121 atcgatgccc gagccgatcc catcccggac agtcatgggg ggcaggaacg gcaagaccag
      181 gtagggtccg gggggtaccc cccagacgcc cagggtctgc ccagtgtcct tgtcgctcgc
      241 ctggaggccg gcctccgtgg cgacatcgaa gaagccggcg agaccgaagg tgctattgag
      301 gaggaagcgc gccagctctt ggcccgcccc ctccaccttg ccttggagga gactgttcac
      361 gaagcgcctg ggcatgccta cgttgtcgag catgcgcccg aggccccgct gcaccgcgtt
      421 ggggaccacc gtgtcccagc cggtcgccac cggctttagc acatagcgat cgagttcgcg
      481 attgaaggta aacatccgct cgttaaatgg ctcccaggga tcgtagtcct ccgatgctgc
      541 tggctctacc tgcggcgccc ctcgcgtctc ttccgcagtt ttttcgtccg ggaccacgtc
      601 gctgacgggc attgccaacg gggcctcgtc cgagacgggc gcgtccctcg gcgactcgga
      661 caaaaaactg ctcgcctcca cggggttcca cccttcctgc ggtccccctg gcgtcgcttc
      721 cgcagtgtct cgggctggca ccgcgtctcc aaggggcgcc gtcggggggg cctcgtcagg
      781 gacggccgct gcgctcggca gcccggacgg gccgctgctg acgttcacgg cggggtccaa
      841 cccctcctcc gccccggcga aacttagggc gcccggaccg atgctaacga gcgtcgtcac
      901 caggacggcc atccaccaca tggatcctcc gatcttcttt acgattctca tttcccccta
      961 ctccatcttc ctcgttctgg gaaatcttgt tgaactgcgg atggtagttc atcgccaggc
     1021 tcacgccgtc gagtcaaacg tccaccgcgt gagccgtcta cctgatgttc atctcatgcc
     1081 tctcagtgat tcgtttcacg gctaggggaa agcaccgatc gtgccaatgc tcacaatccg
     1141 taaggtgtcg aaaaatcgtg gtcgttgctg cgggaggaag acttgcgcac tcgaagagtg
     1201 caactctgca cgtcggcgtg cggaaccaca catgccgcgg atccaggatt tatagctgtc
     1261 gtgatgtgat agcgcatcat gaggggttaa cgctcggact gggagagccc gaggggggta
     1321 gccgcctggc aactgcccga gactcgtgac cgtacccgca ttggagtgta ggcggagggt
     1381 ctgatccagg tgcacggtga tcatgtcgag atggggtctc atgtggttgt agagctgaaa
     1441 tcgcgtgacg ccgaggaggg cgaaacggcg agcatgacta tgtcggagac gcgcaggact
     1501 ggctggaggc ccaagtgtgg gaggagccgt gtactgtcgc ttcgcgagtt gtttcagagt
     1561 gggatgaaca aaagccccct cggacccttg gcccatgcga gccggggacc gacaccggtc
     1621 gctgcttggc ctggcccacc gcggccctgg ggcccgctgc tccctcgccc tgccgggtac
     1681 aggggccgcc tagcccatca cgagtacttc gatttcctgg cgggtcgcct tcgctgcccg
     1741 cagagtctgc gcgatctccg gggcgatggc ctgccagtgc tgctccttag tccagtaggc
     1801 aatccgccgt tgcgccgcct ccgccggcgc gccctcccat tcgaagacgt tgaagtcatt
     1861 ccgcacgtcc cccgccagct cgtggagcat cttgtgcgcg agggtggcta cccggtctcg
     1921 ggaaagccct cgctccgtcg ccaataggct ggcgacgcga tctacgaggg gtacgatgtc
     1981 ctcgtgggtc ataccgtgct cggtttcatg gaagctcatc ggttctccct ccagccccgg
     2041 cggcggccgg ggcccgggct caacggacgg gagggcgcag gggaagcacc ggacacgggt
     2101 ttccgtgcac tacgttcccg gctacgctgc ccagacgagc cgtgctcgca aggaggtccg
     2161 acgtgccttc cggcagtccc tccccctccc gccccgcttc cggatgccaa ggcgtcggtc
     2221 gcgacatcct tcttggcgac atctcccctt gtcgaggcgc gcgatgtccc ctcggccaat
     2281 acccgggccg cttccaccgg ccccagcgga gtcaaagaca gctcaggggc gaagcaggat
     2341 ggtggcagca tcgccgaagt atctattcca gagggtgtcg agggtgccgt cggtcttggc
     2401 ctgccgcaac tgctcgttca cccaatcgcg cagctctcgc tcgcctttgt gcatggcaat
     2461 ggcatagggt tggggccgga acggcctccc caccgctaca agatcgggat actgcttggc
     2521 gagcgccaag atcaggacat catcctctgc gagggcgtcc acctggcccg cccggagagc
     2581 ggccactgct gcggcgacgt tccggaactg cacccgaatg acccctcgga cgactggctc
     2641 tcggacgact ggctcccaga cgccctcctg gatcgtgccc tcgagcaccg ccaccctctt
     2701 gcccggcaga tcaccgatgt cccggataga gctgtcccgg gggacgagca ggagggtacc
     2761 ggagaggaaa tagggctcgg agagggcgaa gacggcggcg cggtcctcgg ttgcggacac
     2821 ggaggcgatc agcatgtcgg ccgatccgct gaggatggcc gtcaggcggt cccctgcgtt
     2881 gaccctcacc agccgcagtt taccctcgtc gcccagcagt tgccgggcca ggtagcgggc
     2941 gaggtccacc tcgaagccgg tgggctggcc ctggccgtcc aaatagctga gtggcggaaa
     3001 atcccatcgt acccccacgg tcagaatccc ccgcgccttc accgcctcca gggtcccggc
     3061 atagccccgg gtcgccccca gcagcgtcag ggcgccgaag agaagtgtca ctaacgtgcg
     3121 cacggcgacc agtctcttac tgtgcattgt gcttcccccc tgttaattcg agatggcggc
     3181 atcccttgct catcctgctc ggaacctcct gccccgcggc atcgtggaag atgagggctt
     3241 ggctctttag ccgctggcag ccagcctatc gcaggtggcc gttgccgccg acgctggcca
     3301 ccagggccgc gacctcgctg gctccatcgg gcagcgcgct gccccgtcgc tggacttcgt
     3361 cggccagtcg gctcagacag cggctgcacg ccagcccgcg atagggccag ccggccacca
     3421 caatccggcc gacggctcgg ggttcgtggc acatctggca gtagcctgga taggcgtcga
     3481 cgaccgtcag aaatctcgcc ccggtgttca tgatcacatc tctctcgagg tccgttcaat
     3541 acactcacgc ggcccgcttg ctgccgcctt tcaccgcaga gagggtggct ccgacgatcg
     3601 agacccccag caccccccac gggaagagga gcagaaaggc gattacaatc ccatgaaccg
     3661 tcacatgatt tctccctggt gcccgtccgc ttccgagtta ggtgtccgac ccggggtgcg
     3721 gcaggaagcc atcagatgtt gctccgcctg cccgtggcag ggaccgcctt gagcaattca
     3781 gggagggcag tgtcattcgc cggatccacg gccgcatagg tccctgacgc cttcccctca
     3841 tccacaaaac caatatccac gtacacatcc tggaccgtcg ccgcgtcggc gaagaccacg
     3901 atcaggatga tgggatccgt gctcagtgac caagtgtctt tcccattgcg tgcatcgacc
     3961 ccaaggagga tggccggggg tgactcttcg gtatcgttca ccagcaggag cattggcaag
     4021 tccggattct cctgcaggtt cgccacaggt acaggcttg
//
LOCUS       DBNGZC010000593         2741 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_932002, whole genome shotgun sequence.
ACCESSION   DBNGZC010000593 DBNGZC010000000
VERSION     DBNGZC010000593.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_932002"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="AC16G0_10335"
     CDS             <1..288
                     /locus_tag="AC16G0_10335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5100046.1"
                     /translation="DEAFGLAALEALSCAVPVIATTVGGISEVVEEGKSGFLLPPGDV
                     DGMVKAALTLLTDPSRHAEFRVAARQRAVSQFDASLIVPQYEAYYHEISGT"
     gene            412..>2741
                     /locus_tag="AC16G0_10340"
     CDS             412..>2741
                     /locus_tag="AC16G0_10340"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR5100047.1"
                     /translation="MGAPIHTSAWQLAPSRILSLDALAALLCLITLTSCATTDEQRFP
                     LDYVSGIRAALQPETAREAFQAYQRMGVDLERQGRFAQAAIAYSNASTSARALGRLQD
                     ALVTSQKAVAAAEQARDPRHLAPALVRLGLVHLSLNAPQRAVSILEKAAQEAAKAGNP
                     NVEASSYLWLSRAYSRLAKRDLAMAYTQKAVAVLEPVILRMRAHGQRLRGERQEGLWN
                     MERNYADAVLDLGGHHVALHELEPARAYFEKALQSGERLGVPQLMANAQFGLGRVALE
                     QQDPQAAIGHLEESLRLSPQPGLAVGTQTLLGRLYQEMGNLPLAERHLRISIAAIEDM
                     RSQLQSEEFRETFVENKMGAYARLIQVLFDQRKLAEAFDMSERARARAVLDLQGNRVC
                     LSQGRRASLIAEEKALQERIAQLKAREALQDGEEADPEAKTRPGRSALQRELDLAREA
                     YGAFLSRVRAQSREQASLMTVEPLTLPEVQALLGPDTVLLEYFVGGGRTLGWVVRRNG
                     LRVVRLRIGDRELTKRISDFRQLIASRGHVEELQQAAGDLFQILVAPAFPAGLPRELL
                     IVPHRTLHYLPFHALMPAPGRYLVQESLVYYLSSASLMQFTREKESTSTTGALAVGNP
                     DLGDPTLNLRYAEREAREVGRMFPQATVLVGKEATKSQTRRYIDAEPLIHLATHADLD
                     GSDPLGSALLLRPVAGDTGRLEVQEVFGLDLHASLVILSACDTSLGKLTQGDEVVGLT
                     RAFIYAGTPSVISTLWMVDDRASYELMQAFYQNLRAR"
BASE COUNT          480 a          932 c          868 g          461 t
ORIGIN      
        1 gacgaggcct tcggcctcgc ggccctggag gccctgagtt gcgccgttcc cgtcattgcc
       61 actacggtgg ggggaatttc ggaggtggtg gaagagggga agagcggctt cctattgcct
      121 ccgggcgatg tggacgggat ggtcaaggcc gcccttaccc tgctcaccga tccgagtcgc
      181 catgccgagt tccgggtggc ggcacggcaa cgcgccgtct cccagttcga cgccagcctg
      241 atcgtcccgc agtacgaggc ctactaccac gagatcagcg gaacctgagc cgacagctct
      301 cccctcccca cccgttttcc ttcttgacag ggccggacct gaaggttaga agaatcgcta
      361 ggattccctg cgacgtgcag ttccgagcta tccgtcggcg acgcaggaaa agtgggggct
      421 ccgatccaca cctcagcttg gcagcttgcg ccttcccgca tcctctccct cgatgccctc
      481 gcagccctcc tctgcctcat caccctcacg agctgcgcga ccacggacga gcagcggttc
      541 cctctggatt atgtgtcggg aatccgtgcc gccctgcagc ccgaaacggc ccgagaggcc
      601 ttccaggcct atcaacgcat gggtgtcgat ctcgagcggc aaggccggtt tgcccaggcg
      661 gccatcgcct attcgaatgc ctccacatca gcgcgagctc tcggacgcct ccaggatgcc
      721 ctcgtcacaa gccagaaggc ggtggcggcg gccgagcagg ctcgagaccc cagacatctc
      781 gcacccgccc tggtccgcct gggactggta catctgagct tgaatgcccc gcagcgggcg
      841 gtgtcgattc ttgagaaggc cgcccaggag gcggcaaagg ccgggaatcc gaacgtcgag
      901 gccagcagct atctctggct cagtcgagcc tacagcagac tcgccaaacg cgacctggcc
      961 atggcataca cgcaaaaggc agtggcggtc ctggagccgg tcatcctgcg catgcgcgct
     1021 catgggcagc gcctccgcgg cgaaaggcaa gaggggctct ggaacatgga gcggaattac
     1081 gcggatgcgg tgcttgatct gggcggccat cacgtggccc tccacgaact ggaacccgct
     1141 cgggcgtatt tcgagaaggc tttgcaaagc ggagagcgtc tcggcgtacc gcagttgatg
     1201 gccaatgccc agttcggtct gggcagggtg gccctggagc agcaagaccc ccaagccgcc
     1261 ataggccacc tggaggaatc cctccggctc tcgccacaac ccggtctggc cgtgggaact
     1321 cagacgcttc tcggacgcct gtaccaggag atgggcaatc tcccgttggc cgaaaggcac
     1381 ctacgcattt ccatcgccgc gatcgaggat atgcgttcgc agctccagtc cgaggaattc
     1441 cgggagacct tcgtcgagaa caagatgggg gcctacgcca ggcttatcca ggttctcttc
     1501 gaccagagga agctggccga ggcctttgac atgagcgagc gggcccgggc ccgggccgtt
     1561 ctcgatctcc agggaaaccg cgtgtgcctg tcccagggcc ggcgcgcatc gctcatcgcc
     1621 gaggagaagg cgctgcagga acggatcgca cagctcaagg cgcgtgaggc ccttcaggat
     1681 ggcgaggagg cggacccgga ggcaaagacc cgccccggcc ggtcagccct ccagcgggag
     1741 ctggaccttg cccgcgaggc ctacggagcc ttcctgtccc gggtccgggc ccagagccgc
     1801 gaacaggcct ccctgatgac ggtcgagccg ctcaccttgc ccgaggtcca ggcgcttctg
     1861 ggcccagaca ccgttctgct ggaatacttc gtcggcggag gtcgcacgct gggctgggtg
     1921 gtccggcgga acgggctacg cgtcgtgcgc ctgagaatcg gagaccgcga gctgacgaaa
     1981 cgcatcagcg atttccggca actcatcgcc tcccgcgggc atgtcgagga gcttcagcag
     2041 gccgcgggcg atctgttcca gattctggtg gctccggcgt tcccggcggg attgccccgc
     2101 gaactcctca tcgtccccca tcgcacgctc cactatctcc ccttccacgc cctgatgccg
     2161 gctcccggcc gatacctggt gcaggagagc ctggtgtact atctgagcag cgccagcctg
     2221 atgcagttca cccgcgagaa agagtcgacc tccacgaccg gcgctctggc cgtggggaat
     2281 cctgacctgg gtgatcccac cctgaatctc cggtacgccg agcgcgaagc ccgcgaggtc
     2341 ggccggatgt ttccccaggc aaccgtgctc gtcgggaagg aagcgacaaa gagccagaca
     2401 cgacgctaca tcgacgccga gcccctgatc catctcgcga cgcacgcgga tctggacggg
     2461 agtgatcccc tgggctctgc cctgctgctc cggcccgtgg caggcgacac gggtcggctg
     2521 gaggtccagg aggtcttcgg cctggacctg cacgcctccc tcgtgatcct ttccgcctgc
     2581 gacacatccc tgggcaagct gacccagggg gacgaggtcg tcggcctcac ccgcgccttc
     2641 atctatgccg ggaccccctc ggtcatcagc accctgtgga tggtggacga tcgggccagt
     2701 tacgaactca tgcaggcctt ctatcagaac ctccgggcac g
//
LOCUS       DBNGZC010000594         3800 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2399183, whole genome shotgun sequence.
ACCESSION   DBNGZC010000594 DBNGZC010000000
VERSION     DBNGZC010000594.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2399183"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3800
                     /locus_tag="AC16G0_10345"
     CDS             <1..>3800
                     /locus_tag="AC16G0_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006274635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100048.1"
                     /translation="HDLHAKYGSLGDTYDPNWHYTLTQAEVDAITASIHVYTEEELLY
                     AMNSGLLKPVAKTTTTFEDPNAKGVNVSLVTSQDIGTTSGEEVIDLSQKPVTLTPEQR
                     VILAAAEREDVHYLGNAVTATVDFTHTESTDTTTDTISRSSGSWITDGFAAGMRIRVE
                     GDSLNADPDGSTVYYIDSVSDSTITLETGSKLITEVGRTVTITPEIVDPLANGNLIKQ
                     IVVDQREDVDLEATGWISVDAGADAYIGSEMDMNLDHVEAGGKVRIKGAKGIYNNAQV
                     AGRPNVESQDLILEAGGSAIGTDATPSDGDPFNGTPLTVDLVDGSRLTARAVNDIYIY
                     EHNSTGTAGSLSLATVYAESGGAYLMSDGSIVDGANDDFTNIQANTVELKAVGGGIGE
                     SGDYLETDAVGAGTLKAMAQGTIRLWESEGNMNVKRIESLAGDVDLKAQIAILDTDDT
                     PAPDVLGSSINLTASLGTIGMTGNDLDIDTHGAGLLSSSSFLNSYIIETAGNLNIDQM
                     STGAGNTAFIAAPDGSILNGRTSGGNVLSGKAYLVAKQDIGQDGKPLATEVGGIEGKA
                     ILGSVWVANQGPLTEGGVIDSGDPGVVSGGGITVTASSPITVSENIVSGGDITLTSTD
                     KAAAGDDLTILSDISIVSATYDFTTHTWVLADGTITLNSGDNFTMQPGAKVSTLGPVV
                     IRGDYGNADPGIGAAIVPRGQIVAQSVELSGEADGDVFDLRSMTVPASVAGGGGSDTL
                     IGPDGDNTWEITGPNSGNLNGEYPFESVENLTSGNGDDTFYFRGQGSVGGIVDGGAGF
                     DTLNFLESDFIQEAAEDFGDVDLPIGDAVVPFAATGGITRIEDIKVILLAEVQADGTL
                     LLNMGPRAAERLAINTEDGDETFVLAHVNGDPTTAAGETVRVTAFGITLDYDGVRSIR
                     GSGGQGNDTIILASDILAPAELDGGPGSDNLTGGGGDDILLGDAGLIIRAYETNGTPR
                     LNPDGSWHKDVVQTDLARITGRVDLDGLIWTDLDRALAEAIVQADLLLLTGAYDADGS
                     LHLLPQVPAPCWPPHDEWDTELLLLTLLADGDDTLDGGPGNDALYGQRGHDSLTGGAG
                     DDFLEGGTGNDHLNGDEGADWLVGDQATVLTEDGIFPNVAHAVQLLPGGIEAAAGITL
                     PSDGTVLLPVTAVVPGHDVDPAQGLLAQLRGVPGAPLDNTLSRTDGTHLVPFATLVPG
                     FVGHLDLVAGNDTLMGGAGDDVLTGDNATVLAPSLPFTDTRMEQALSLTADFLDVADD
                     LGDL"
BASE COUNT          737 a         1244 c         1248 g          571 t
ORIGIN      
        1 cacgatctcc atgccaagta cggaagcctc ggcgacacgt acgatccgaa ctggcactac
       61 acgctgacgc aggccgaagt cgacgccatc acggcgagca ttcatgtgta caccgaggag
      121 gagctgctct acgcgatgaa ctcgggcctc ctcaagccgg tggcgaagac cacgaccacg
      181 tttgaggatc cgaacgccaa gggcgtcaac gtgagcctgg tcacctccca ggacatcggc
      241 acgaccagcg gcgaggaagt catcgacctg tcccagaagc ccgtgacgct gacgcccgag
      301 cagcgggtga tcctggcggc ggccgagcgg gaagatgtgc actatctggg taacgcggtg
      361 acggccacgg tcgacttcac acacaccgaa tctacagaca ccactaccga caccatcagc
      421 cgctccagcg ggagctggat cactgacggc tttgcagccg ggatgcggat cagggtagag
      481 ggcgactccc tgaacgcaga tcccgacggc agcacggtct actacattga cagcgtttcc
      541 gatagcacca tcaccctgga gacgggctcc aagctgatca ccgaggtcgg caggaccgtg
      601 accatcaccc cggagatcgt cgacccgctg gcgaacggga acctgatcaa gcaaatcgtg
      661 gtcgaccagc gtgaggatgt ggacctggag gcgacgggct ggatcagcgt ggacgccggc
      721 gccgacgcct acatcggttc cgaaatggac atgaatctcg accacgtgga ggcgggcggc
      781 aaggtccgga tcaagggtgc gaaggggatt tacaataacg cccaggttgc cggcaggccc
      841 aacgtcgaga gtcaggatct gatcctcgaa gcaggcggaa gcgcgatcgg gacagacgcc
      901 acgccttcgg atggcgaccc attcaacggc acgcccctca ccgtcgacct cgtcgatggc
      961 tcccgcctca ccgcgagggc ggtcaacgac atctacatct acgagcacaa cagcacgggc
     1021 accgcgggca gcttgtccct ggccaccgtc tacgcagagt cgggcggagc ctacctcatg
     1081 tcggacggct ccatcgtgga tggggcgaac gatgatttca cgaacatcca ggccaacacg
     1141 gtggagttga aggcggtcgg cgggggaatc ggcgagtcgg gcgactacct ggagacggat
     1201 gcagtggggg ccgggaccct gaaggcgatg gctcagggga ccatccggct ctgggaatcc
     1261 gaaggcaaca tgaatgtgaa gcggatcgaa tcgctggcgg gtgacgtcga cctgaaggcg
     1321 caaatcgcga tcctggacac ggacgacaca cccgcaccgg acgtcctcgg cagcagcatc
     1381 aatctgaccg cgagcctcgg cacgatcggc atgaccggga acgatttgga tatagacacg
     1441 cacggtgccg ggctcctctc ctcttccagc ttcctcaaca gctacatcat cgagacggcc
     1501 ggcaacctca acatcgacca aatgagcacg ggcgcaggaa acacggcctt cattgcggcg
     1561 ccggacggga gcatcctgaa cggccggacc agcggcggca acgtcctttc gggcaaggct
     1621 tacctggtcg cgaagcagga catcggtcaa gatgggaagc cgctcgcgac cgaggtgggc
     1681 ggcatcgagg gcaaggccat cctgggcagc gtgtgggtcg ccaaccaggg cccgctcacc
     1741 gagggcggcg tgatcgatag tggcgacccg ggcgtggttt cgggcggggg gatcaccgtg
     1801 accgcctcca gccccatcac ggtttccgag aacatcgtct cgggtggcga catcacactg
     1861 acctcgaccg acaaggccgc cgcgggtgac gacctcacca tcctctcgga catctccatc
     1921 gtctcggcga cctacgactt cacgacccat acgtgggtcc tcgcagacgg gacgatcacc
     1981 ctaaactccg gggacaactt caccatgcag cccggggcga aggtgagcac gctggggccg
     2041 gttgttatcc gcggtgacta cggcaacgcc gatccgggca tcggcgccgc catcgtcccg
     2101 cgaggacaga tcgttgccca gtcggtcgag ctgtccgggg aagcggacgg cgacgtgttc
     2161 gatctccgca gcatgaccgt accggccagc gtcgcgggtg ggggaggcag cgacaccctg
     2221 atcgggcccg acggggacaa tacgtgggag atcaccggcc ccaacagcgg aaacctgaac
     2281 ggcgaatatc cctttgaatc cgtcgagaac ctcacgtccg gcaacgggga cgacaccttc
     2341 tatttccgtg gccaggggag cgtgggtgga atcgtcgacg gcggtgccgg cttcgatacg
     2401 ctcaacttcc tggagtcaga tttcattcag gaggcggccg aagatttcgg tgatgtggac
     2461 ctgcccatcg gggatgcggt ggtgcccttt gcagcaacgg gcgggattac ccggatcgag
     2521 gacatcaagg tcattctcct ggctgaggtc caggcggatg gcacgctgtt attgaacatg
     2581 ggcccgcggg cggccgagcg actcgccatc aacaccgagg acggcgacga gacgttcgtc
     2641 ctcgcgcacg tcaacggcga tcccacaacc gcggccggtg agacggtccg ggtcaccgcg
     2701 ttcggcatca cgctggacta cgacggggtc cggtccattc gtggctcggg gggccagggg
     2761 aacgacacca tcatcctcgc cagtgacatt ctggcacccg ccgagctaga cggtggaccc
     2821 gggagcgata atctcaccgg tggcgggggc gacgacatcc tgctgggcga tgccggcctc
     2881 atcatccggg catacgagac caacggcacc ccgcgcctga acccggacgg ctcgtggcac
     2941 aaggatgtcg tccagaccga tctggcgaga atcaccggcc gcgtggacct ggatgggctc
     3001 atctggaccg atctcgaccg cgccctggcc gaggccatcg tgcaggccga cctcctcctg
     3061 ctcaccgggg cgtatgacgc cgatggcagc ctgcatctgc tgccgcaggt ccccgccccc
     3121 tgctggccgc ctcacgacga atgggatacc gaactgctgc tcctgacgct cctcgccgat
     3181 ggggacgata ccctcgacgg cggccccggg aacgacgccc tctatggcca gcgggggcac
     3241 gactccctca ccggcggggc cggggacgac ttcctcgaag gtgggaccgg caacgaccac
     3301 ttgaatgggg acgagggcgc cgactggctg gtgggggatc aggcgacggt tctcaccgag
     3361 gatggcatct tccccaacgt ggcccacgcg gttcaactcc tgcccggcgg gatcgaggcc
     3421 gccgcgggga tcacccttcc gagcgacggc acggtcctcc tgccggttac cgcggtggtg
     3481 cccgggcacg acgtggatcc ggcccaaggc ctcctggcgc agctccgggg ggtgccgggt
     3541 gcccccctgg acaacaccct gtcacggacc gacggcactc acctcgtccc cttcgccacg
     3601 ctggtgccgg gcttcgtggg gcacctcgac ctcgtggcgg ggaacgacac cctcatgggc
     3661 ggggcgggcg atgacgtgct cacgggggat aacgccacgg tcctggcccc atcccttcca
     3721 ttcaccgata cccggatgga gcaggccctc agcctcacgg ccgacttcct ggacgtggcg
     3781 gacgatctgg gcgacctgat
//
LOCUS       DBNGZC010000595         1649 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2399297, whole genome shotgun sequence.
ACCESSION   DBNGZC010000595 DBNGZC010000000
VERSION     DBNGZC010000595.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2399297"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..135
                     /gene="acpP"
                     /locus_tag="AC16G0_10350"
     CDS             <1..135
                     /gene="acpP"
                     /locus_tag="AC16G0_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012982657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR5100049.1"
                     /translation="DSLDTVELVMALEEEFGIEIPDEDAEKITTVRLAINYIQEHKKD
                     "
     gene            232..1464
                     /gene="fabF"
                     /locus_tag="AC16G0_10355"
     CDS             232..1464
                     /gene="fabF"
                     /locus_tag="AC16G0_10355"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR5100050.1"
                     /translation="MRRVVVTGVGAVAPNGIGIENFWPNLVRGVSGVAPITRFDASKH
                     DAQIAGEVKGFDPLQWMEKKEVRKMDLFIQFAIAAAQMAYDDSGLKVTDENRERIGVF
                     VGTDMGGIPALEESHKILLERGPGRISAFFIPSIITNMGSGQISMRFGMKGPNSCVCT
                     ACATGNHAIGDSFRLIQRGDADVMVAGGAEAVITPLTIGGFSAMKALSTRNSEPTRAS
                     RPFDKGRDGFVMGEGSGIMVLELLEHAQRRGARIYAELVGYGMSADAYHITLPAPDGA
                     VRSMRLTLEDAKVDPSVVEYINAHGTSTPAGDVNETQAIKAVFGEHARRLPVSSTKSM
                     TGHLLGAAGGIESVITVLAIFKRTLPPTINQETPDPECDLDYIPNTARPAEVQYALTN
                     SFGFGGTNASLLFKRFEE"
BASE COUNT          311 a          534 c          546 g          258 t
ORIGIN      
        1 gattccctgg acacggtcga gctggtgatg gcgctggagg aggaattcgg gatcgagatt
       61 cccgacgagg acgcggagaa gatcaccacg gtccgcctgg cgatcaacta tatccaggag
      121 cacaagaagg actagcaccc gtgaagggtg ccggtgtgcg gagaccggtc gcgcgcccgg
      181 caaggaatgt acgaggcccg gcaggcggtc aagagagaca ggggtaggac catgaggcgt
      241 gtggtggtca ccggcgtggg ggcggttgcg cccaacggaa tcgggatcga gaacttttgg
      301 ccgaacctcg tccggggggt ctcgggcgtg gcgcccatca cccggttcga tgcgagcaag
      361 cacgacgccc agatcgcagg tgaggtcaag ggcttcgacc cgctccagtg gatggagaag
      421 aaagaggtcc gcaagatgga cctcttcatt caattcgcca tcgccgccgc ccagatggcg
      481 tacgacgact ccggcctcaa ggtcacggac gagaaccggg agcggatcgg cgtcttcgtg
      541 ggcaccgaca tggggggcat ccccgccctg gaggagtcgc acaagatcct cttggagcga
      601 gggccggggc ggatcagcgc cttcttcatt ccctccatca tcacgaacat gggctcgggt
      661 cagatctcca tgcgcttcgg catgaagggt cccaactcct gtgtgtgcac ggcctgcgcc
      721 acggggaacc atgccatcgg cgactccttc cgtctgatcc agcgtggcga cgcggatgtg
      781 atggtggccg ggggggccga ggcggtcatc acccctctca ccatcggcgg gttctctgcc
      841 atgaaagccc tctccacgcg caacagcgag cccacccgcg ccagccgccc cttcgacaaa
      901 gggcgggacg gcttcgtgat gggcgagggc tcggggatca tggtgctgga gctgttggag
      961 cacgcccagc ggcgcggcgc caggatctat gccgagctcg tggggtacgg catgtccgcc
     1021 gatgcctacc acatcacgct gccggccccc gatggcgcgg tgcgctccat gcgcctgacg
     1081 ctcgaggacg ccaaggttga tccctccgtg gtcgagtaca tcaacgccca cgggacctcg
     1141 acgccggcag gcgacgtgaa cgaaacccaa gccatcaagg ccgtctttgg cgagcatgcc
     1201 cgcaggctgc ccgtcagctc caccaagtcc atgaccggcc acctcctggg ggcggccggc
     1261 ggaatcgaat cggtgatcac cgtcctggcg atcttcaagc ggacgctgcc gcccaccatc
     1321 aaccaggaaa cgcccgatcc cgagtgcgac ctggattaca tccccaacac ggcccggccg
     1381 gcggaggtgc agtacgccct gaccaattcc ttcggattcg gcgggaccaa cgccagcctc
     1441 ctgttcaagc ggttcgaaga atagatctca ggcgcaggca aggacgggct gatggcgcca
     1501 ctcaccgggg tggcgacccg gatacgccag acccacggaa gggggccctg gccgggaccc
     1561 gatcactgac accgcaagtg ggtgggtcca tccgatccag tggatggact tctccctctg
     1621 actgcctccc ctggttcatc tgcaacgct
//
LOCUS       DBNGZC010000596         2540 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_347513, whole genome shotgun sequence.
ACCESSION   DBNGZC010000596 DBNGZC010000000
VERSION     DBNGZC010000596.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_347513"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="AC16G0_10360"
     CDS             complement(<1..318)
                     /locus_tag="AC16G0_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HIR5100051.1"
                     /translation="MTDLSTNYLGLALKNPIIASASPLCEDLGTVRRMEDAGAAAVVM
                     HSLFEEQLSLESNDLDRFLSHGTESFPEALTYFPDMMQYNLGPDGYLEHLRRVKAAVA
                     IPVI"
     gene            complement(315..>2540)
                     /gene="nifJ"
                     /locus_tag="AC16G0_10365"
     CDS             complement(315..>2540)
                     /gene="nifJ"
                     /locus_tag="AC16G0_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008386030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HIR5100052.1"
                     /translation="TDNYAQGYFVYDSKKSGALTISHLRFGPKPIHSTYLISQASFVA
                     CHQFSFLERLDVLKAAATGATFLLNSLYGPDEVWDHLPTTVQRQIIEKKLRFYVIDAY
                     KVAEKTGMGVRINTVMQTCFFAISGVLPREEAIAAIKHAIEKTYGKRGEAVVKKNFAA
                     VDAALEHLHEVKVPGQVTSRFDLRPAVPAQAPEFVQKTLAKIIANEGDALSVSAFPVD
                     GTFPTGTTQWEKRNIALEIPVWDTDVCIQCGKCILVCPHAVIRAKVYSPDLLKNAPAT
                     FKSAPARWREFKDMKYTLQVAPEDCTGCELCVEVCPAKNKQETRLKAINMAPQPPIRE
                     AERENWDFFLSLPEMNRDALNLAQVKDVQLLEPLFEFSGACAGCGETPYIKLMTQLFG
                     DRAVVANATGCSSIYGGNLPTTPYTFNQEGRGPAWNNSLFEDNAEFGLGLRLATDKQN
                     QFAQELLKRLSAQLGDELVNGLLEADQSNEAGVKAQRDRVKALKEKLKGIDTPEARNL
                     CSVADALVKKSIWIVGGDGWAYDIGYGGLDHVLASGYNVNVLVLDTEVYSNTGGQMSK
                     ATPRGAVAKFAAGGKPRPKKDLAMIAMTYGNIYVARVAFGMNDMHTVRAFLEAEAYDG
                     SSLIIAYSHCIAHGYDLKHGLEQQKAAVQSGHWPLLRYNPDLVKQGKNPLILDSKAPT
                     MTLEKFESNETRFTMLNHSDPAAAKQLLETAQAEVTARWRLYEHLAAMPGNGKPEEKA
                     P"
BASE COUNT          405 a          799 c          824 g          512 t
ORIGIN      
        1 gatgaccggt atggccacgg ccgccttcac ccggcgcagg tgctccaggt agccgtccgg
       61 acccaggtta tattgcatca tgtccgggaa gtaggtcagc gcctcgggga agctctcggt
      121 gccgtgggac aagaagcgat ccaggtcgtt actctccagg ctcaattgct cctcgaagag
      181 cgagtgcatc accaccgccg ccgccccggc gtcctccatc cggcgcacgg tgcccaggtc
      241 ctcgcacaga ggcgaagcgg aggcgatgat cgggttcttc agggcgagac ccaaatagtt
      301 tgtggacagg tctgtcatgg tgccttctcc tcgggcttgc cgttgcccgg catggcggcc
      361 aagtgctcgt agaggcgcca gcgcgccgtc acttcggcct gagcggtttc cagcagttgc
      421 ttggccgcgg cggggtcgct gtggtttagc atggtgaagc gcgtctcgtt gctctcaaac
      481 ttctccaggg tcatcgtcgg tgccttgcta tccaggatga gcgggttctt cccctgcttg
      541 accaggtccg ggttgtatcg caacagcggc cagtggcccg actggaccgc agccttctgc
      601 tgctccaacc cgtgcttgag gtcgtagccg tgggcgatgc agtggctgta ggcgatgatg
      661 agcgaggagc catcgtaggc ctcggcctcc aggaaggccc gcaccgtgtg catgtcgttc
      721 atgccgaagg cgacccgcgc cacgtaaatg tttccgtagg tcatggcgat catcgccagg
      781 tccttcttgg gccggggctt cccgcccgcc gcgaacttcg ccacagcgcc acgcggggtg
      841 gctttggaca tctggccgcc ggtgttggag tagacctcgg tgtccagcac caggacgttc
      901 acgttgtagc ccgaggccag gacgtgatcc agaccgccgt agccgatgtc gtaggcccag
      961 ccgtctccgc ccacgatcca gatgctcttc ttgaccaggg cgtccgccac gctgcacagg
     1021 tttcgtgcct cgggagtatc gatccccttc aatttctcct tgagggcctt cacccggtcc
     1081 cgctgcgcct tgacgccagc ctcgttggac tggtcggcct caagcagccc gttcaccagt
     1141 tcgtcgccga gctgcgcaga cagccgcttg aggagttctt gggcgaactg gttttgcttg
     1201 tccgtcgcca gacgcagccc caatccgaat tcggcgttat cctcgaagag ggagttgttc
     1261 caggccggcc cgcgcccctc ctggttgaac gtgtacgggg tggtgggcag gttgccgcca
     1321 tagatcgaag agcagccggt ggcgttggcg acgacggccc gatcgccgaa gagctgtgtc
     1381 atgagcttga tgtagggcgt ctcgccgcag ccggcacagg cgccggagaa ctcgaagagc
     1441 ggctccagga gctggacatc cttcacctgg gccaggttga gggcgtcccg attcatctcg
     1501 gggaggctga ggaagaagtc ccaattctcc cgctccgcct cgcggatggg gggctggggc
     1561 gccatgttga tggccttgag ccgggtctcc tgcttgttct tggccgggca cacctccacg
     1621 cacaattcgc agcccgtgca gtcctcgggt gccacttgca gggtgtactt catgtccttg
     1681 aactcgcgcc agcgcgcggg ggcggacttg aaggtggccg gcgcgttctt gagcaagtcc
     1741 ggcgaataca ccttggcccg gatcaccgca tgcgggcaga ccaggatgca tttcccgcac
     1801 tggatgcaga cgtccgtatc ccacaccggg atctccaagg cgatgttccg cttctcccac
     1861 tgggtggtgc cggtcggaaa cgtgccgtcc accgggaagg cgctcaccga cagggcatcc
     1921 ccttcgttgg cgatgatctt ggccagggtc ttttggacga actcgggcgc ctgcgccggg
     1981 accgcgggtc gcaggtcgaa gcggctggtc acctggccgg gcactttcac ctcgtgcagg
     2041 tgctccaggg cggcatccac ggcggcgaag ttcttcttga ccacggcctc gccccgcttg
     2101 ccataggtct tttcgatggc gtgcttgatg gcggcgatgg cttcctcgcg cggaagcacg
     2161 ccgctgatgg cgaagaagca ggtctgcatg acggtgttga tgcgcacccc catgccggtc
     2221 ttctccgcca ccttgtaggc gtcgatcacg taaaagcgca gcttcttctc gatgatctgc
     2281 cgctgcaccg tcgtcggcag atgatcccag acctcgtcgg gtccatacag gctgttgagc
     2341 aggaacgtcg cccccgtggc ggccgccttc agcacatcca gccgctccaa gaagctgaac
     2401 tggtggcagg ccacaaagct cgcctggctg atgaggtagg tggaatgaat gggcttggga
     2461 ccaaagcgca ggtgcgagat ggtcaaggcg ccggacttct tcgagtcgta gacgaagtag
     2521 ccttgggcgt aattgtccgt
//
LOCUS       DBNGZC010000597         3359 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1912678, whole genome shotgun sequence.
ACCESSION   DBNGZC010000597 DBNGZC010000000
VERSION     DBNGZC010000597.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1912678"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="AC16G0_10370"
     CDS             <1..822
                     /locus_tag="AC16G0_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase family protein"
                     /protein_id="HIR5100053.1"
                     /translation="TAAPYGYRLEGMNPGETVGQAASRMLEETILREGPETVAAVIAE
                     PVQGAGGVIVPPDDYFPLVRKVCTKHDVLLIADEVITGFGRTGSWFALTRWGVQPDIL
                     SFAKGITSGYLPLGGIMVSRPILDAMLSAPYADRWMHAYTYSGHATCCAVGLRNLEIL
                     EKEGLVENAAKMGARLLAGMRTLMDLKAVGEVRGLGLMVGVELVADRATKANFDPAKQ
                     IVNKVRAELEARGVFTRSMRDILAFAPPLVITEAQVDKLVESTRGAIAAVLPERA"
     gene            complement(915..1535)
                     /locus_tag="AC16G0_10375"
     CDS             complement(915..1535)
                     /locus_tag="AC16G0_10375"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HIR5100054.1"
                     /translation="MLAVGLFKLGVAARPLQTELGVTYKTAWTMLDRIRQAVGADPVL
                     RRLSGEVEIDDTYYGGHRKGKRGRGAAGKTPVVGIRQRGGRVRSVVVSALDAQTVQTV
                     IRQHVRRGSRVYTDGLNVYQGLAALGYRHATVDHTEHFVAPHGVHTQGIESHWAHTKP
                     GLRARHRKLSPQHLPKYLAAADFKRNTVHEPDFIHLVLQRLVSHTL"
     gene            1513..1662
                     /locus_tag="AC16G0_10380"
     CDS             1513..1662
                     /locus_tag="AC16G0_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100055.1"
                     /translation="MNSPTASTHLESGVRVFAKIVPVVSEKWHRHRRHRYSNRPAAVM
                     RQIRL"
     gene            complement(1617..1790)
                     /locus_tag="AC16G0_10385"
     CDS             complement(1617..1790)
                     /locus_tag="AC16G0_10385"
                     /inference="COORDINATES: protein motif:HMM:NF024170.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR5100056.1"
                     /translation="MRNKQLPSIGLYAYRTILASEQLCHRLLRRIRWPKGVRCPRCSY
                     SRIWRMTAAGRLE"
     gene            2352..>3359
                     /locus_tag="AC16G0_10390"
     CDS             2352..>3359
                     /locus_tag="AC16G0_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006157447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR5100057.1"
                     /translation="MKPILRRDFLKTVGTLVAAGGVLGQVGRVLAAEDPILAGRPLVR
                     YPEKTDLILLTSRPPQLETPMKYFERAITPNEAFFVRYHIFPIPTSVDLATWRLKVTG
                     HVGNPLDLSMDELKTKFPRAVVTAVNQCSGNSRGRFSPRVLGGEWGDGAMGNAEWAGV
                     RLREILTTAGVRPGAVEVSFNGLDQPAFPSVPDFVKSLAIDRIMDDPDVIVAYEMNGQ
                     PLPMLNGYPARLVVPGWYATYWVKNLSDITVLDKPFEGFWVKPAYRIPDNPCGCITPG
                     TAPKRTVPINRMTVRSFIIVPENGAKLPAGGPVVLKGIAFDGGYGIREVEVSDNGGAT
                     WH"
BASE COUNT          581 a         1026 c         1137 g          615 t
ORIGIN      
        1 acggccgctc cctacggcta ccgacttgaa gggatgaatc cgggcgagac cgtgggccag
       61 gccgcgtccc gcatgctgga agagaccatc ctgagggagg ggcccgagac cgtggccgcc
      121 gtcatcgccg agcccgtgca gggagcggga ggcgtcatcg tcccacccga cgactacttc
      181 ccgttggtcc ggaaggtctg caccaagcac gacgttctcc tcatcgccga cgaggtcatc
      241 accgggttcg gccgcaccgg atcctggttt gccctgacgc gctggggggt tcaacccgac
      301 atcctatcct tcgccaaggg catcaccagc ggctatctgc ccctgggcgg tatcatggtg
      361 tcgcgaccca tcctggacgc gatgctctcg gcgccttacg cagaccgctg gatgcacgcc
      421 tatacgtatt cgggtcacgc gacatgttgc gcggtcggcc tgcggaatct ggaaatcctg
      481 gagaaggagg gcctggtgga gaacgcggcc aagatggggg cgcggctcct ggccgggatg
      541 cgaaccctga tggacctgaa ggcggtcggg gaggtgcgcg gtctgggtct tatggtgggc
      601 gtggaactgg tggccgaccg cgccaccaag gccaacttcg accccgccaa gcagatcgtc
      661 aacaaggtga gggcagagct cgaagctcgg ggtgtgttca cccggagcat gcgagacatc
      721 ctggcctttg cgccacctct ggtgatcacc gaggcacagg tggacaagct cgtggagagc
      781 acccggggtg ccatcgcggc ggtgctgccc gagcgagcgt gaagagccag agtgtgttga
      841 gttcgtcgag tctatcgatg gaagctagtg tcctgtccct gaagttgact acataatata
      901 tgggacttat ccccttagag ggtatgactc acgagccgtt ggagcacaag atggatgaag
      961 tccggctcat ggactgtgtt ccgtttgaag tccgcggccg caagatactt cgggaggtgc
     1021 tgtggactca gcttgcgatg gcgggctcgc aggcccggct tcgtgtgcgc ccaatggctc
     1081 tcaatcccct gcgtgtgaac cccgtgcggt gccacgaagt gctccgtgtg atcgaccgtc
     1141 gcatgccggt acccgagagc cgccagcccc tggtagacgt tcaagccatc ggtgtacacc
     1201 cgactcccgc gccgcacgtg ctggcggatc acggtctgga ccgtttgggc atccagggcg
     1261 gacacgacga cgctgcgcac gcggcccccg cgctgtcgga tcccgacgac gggcgtcttc
     1321 ccggccgcgc cccggccgcg tttccccttc cgatgccccc cgtagtaggt atcgtcaatc
     1381 tcgacctcgc cgctgaggcg ccggaggacc gggtcggccc ccacggcctg gcggatgcga
     1441 tcgagcatgg tccacgccgt cttgtaggtc acgccgagct cggtctggag cgggcgggcc
     1501 gcgaccccca gcttgaacag cccgacggcc agcacccact tggagagcgg cgtccgggtc
     1561 ttcgcaaaga tcgtcccggt ggtgtccgag aagtggcacc gacaccgacg acaccgctac
     1621 tccaatcgcc ctgccgcggt catgcgccag atccgactgt agctgcaccg cgggcaccgc
     1681 acacctttcg gccaccggat ccgacgcagc aggcgatggc acaactgctc actcgcgagg
     1741 atggtgcgat acgcgtacag cccaatcgat ggtaactgct tgtttcgcat gccttagagg
     1801 ctaccatctt taccctctaa ggggataagt cccttccctt atgccgccgg accggcaggc
     1861 actgcaggcg gatctgggaa gtgagcgggt gatggccgga ggtgatgcgg cgggctaata
     1921 ttgacaccga tagattagac gggtagcttc cttccaagga agcggaacct atctgggtga
     1981 aggggggagt gatgccgacc gacgggactc cggccgtggg cgctggcaag gtctctccta
     2041 cgcctgagtt tgtccggccc tttgaggagt cgtgatcctc ggagggccgt tgtgtttagg
     2101 gggcgtttct gctgggcgac gccaggggag ggccggatgg atttgcccgt caggggctgc
     2161 gtcgccccgt gccgatttcg cccgcgtgac ctcgtttctc gcgccgctgc cgtgcaggca
     2221 ggcaagactg ccaatcgtct caatgcatgg cggcgactcg tggaggcatt cgaggggttg
     2281 gttcggtgga tgccctgtcg actgtcatcg taggacccgc cgaagcggat tgggcggggg
     2341 gaggcgaatc catgaaaccg atcttgcgga gggactttct caagacggtg gggacgcttg
     2401 ttgccgcagg gggcgtgctg gggcaggtgg ggcgggtctt ggcggcggag gatccgatct
     2461 tggcggggag gcctctcgtt cgataccccg aaaagacgga tctgatcctc ctcacttcac
     2521 ggccgcccca gctcgagacg ccgatgaagt acttcgagcg ggcgatcaca cccaacgagg
     2581 cgttcttcgt tcgctatcac atctttccga tccccaccag cgtggatctc gccacctgga
     2641 ggctcaaggt tacgggccat gtgggcaatc cgctagatct ctcgatggat gagttgaaga
     2701 ccaaattccc gcgggcggtg gtgacagcag tgaaccagtg ctccgggaac agccgcggcc
     2761 ggttttcgcc ccgagtattg ggcggagaat ggggcgacgg tgccatgggg aacgccgaat
     2821 gggccggggt gcggctgcgc gagatcctga caacggcggg cgtgcgaccc ggcgccgtgg
     2881 aggtgagctt caacgggctg gatcagcctg catttcccag cgtgcccgat ttcgtgaaga
     2941 gtctcgccat cgatcggatc atggatgacc cggatgtcat agtggcctac gagatgaacg
     3001 gccagccgct cccgatgctg aacggctatc cggcgcgact ggttgtcccg ggctggtacg
     3061 cgacgtactg ggtcaagaat ctatcggaca tcacggtgct ggacaagccg ttcgagggat
     3121 tctgggtgaa acccgcctac cggattccgg ataacccatg cggctgcatc acgcccggga
     3181 cagctccgaa gcggaccgtt cccatcaacc ggatgaccgt ccggtccttc atcatcgtcc
     3241 ccgagaacgg ggccaagctc cccgccggtg gtccggtggt gctgaagggg atcgccttcg
     3301 acgggggcta cgggattcgg gaggtcgagg tatccgacaa tggcggcgcc acctggcat
//
LOCUS       DBNGZC010000598         3291 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2596292, whole genome shotgun sequence.
ACCESSION   DBNGZC010000598 DBNGZC010000000
VERSION     DBNGZC010000598.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2596292"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..444
                     /locus_tag="AC16G0_10395"
     CDS             118..444
                     /locus_tag="AC16G0_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018435888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF59 domain-containing protein"
                     /protein_id="HIR5100058.1"
                     /translation="MDDTAINTMVIEAQAVEAIRTCFDPEIPVNIYDLGLIYDIKVEP
                     SGAATIRMTLTSPACPSAASLPEEVRQKVEGIAGVSSVSVELVWDPPWDPSRMSEVAK
                     LQLGML"
     gene            469..684
                     /locus_tag="AC16G0_10400"
                     /pseudo
     CDS             469..684
                     /locus_tag="AC16G0_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015716251.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
     gene            1278..2033
                     /locus_tag="AC16G0_10405"
     CDS             1278..2033
                     /locus_tag="AC16G0_10405"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017727273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5100059.1"
                     /translation="MKLKDRVALITGSSTGIGRATALALAREGADVVVTYITNRDKAE
                     EVVEDIQTMGRRAVCRRVDVTKAPEIERLVKGALRAFGRVDILVNNVGGIVRRSPILA
                     MSERAWDLTVTLNLKSTFLCSRAVIPFMIKQKWGRIINMSSIAARLGGAGGLYPYAVA
                     KAGVIALTKGLARELAPAGITVNAIAPGAIDTPFYSRVSPLFDLRQILPNIPVGRVGT
                     AEEVAKVIAFLASDEASYIIGQTIDVNGGWILP"
     gene            2130..2711
                     /locus_tag="AC16G0_10410"
     CDS             2130..2711
                     /locus_tag="AC16G0_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HIR5100060.1"
                     /translation="MAAKKILMLVGDYVEDYEVMVPFQALQMVGHTVHAVCAGKKSGE
                     RVRTAVHDFEGDQTYSEKPGHSFTLNASFDQVRPETYDALVIPGGRAPEYIRLNQDVL
                     KAVRHFAEANKPIASICHGAQLLAAAGVIEGKRCSAYPAVGPEVTRAGGKFMEIPVDA
                     AHVDGNLVTAPAWPAHPAWLAKFLEVLGTKITL"
     gene            2868..>3291
                     /locus_tag="AC16G0_10415"
     CDS             2868..>3291
                     /locus_tag="AC16G0_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018040532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5100061.1"
                     /translation="MAAPREQWLRLTAEDPLEPALPICDPHHHLWDRAEERYLLDELL
                     QDVRSGHNIVSTVFIECRSMYRTDGPEEMKPIGETEFVEGITAQSASGRSGPTRAAAG
                     IVGYANLTLGAAVAAVLEGHRAASPNRLRGIRHICVWDT"
BASE COUNT          612 a          972 c         1158 g          549 t
ORIGIN      
        1 gtggcacacg ctgcacgccg ccctggaggg aaaggacgag ccggtctcga cggagtaacg
       61 gtggttgggc agtcgtgcac tccggttctc aagcgttgca ggctgcgaga ggaagccatg
      121 gacgatacgg cgatcaacac catggtcatc gaggcacagg ccgtcgaggc gatccggaca
      181 tgcttcgatc cggagatccc cgtcaacatc tacgacctgg gattgatcta cgacatcaag
      241 gtggagccgt cgggagcggc gacgatccgg atgacgctca cgtcacctgc ctgtccgtct
      301 gcggcgtctc tgcctgaaga ggttcgccaa aaggtggagg gcatcgcggg cgtctcgtcc
      361 gtgagcgtcg agctagtctg ggacccgccc tgggatccaa gccggatgtc cgaggtggcg
      421 aagcttcagc tcgggatgtt gtagcgccag gctggggagc ggggagcgat gccgtacgtc
      481 gtgacggagc catgcatcag cgtcgaagag cacgcctgcg tggatgcctg tccggtggag
      541 tgcttctacc aggcggaaga ccaactcctg atccatcccg aggagtgcat cgattgcggg
      601 gcgtgcgtgc cggagtgccc cgtgaatgcc atcttcgaag tgagcaacgt cccggacaag
      661 tggaagagct acatcgtgaa gaatacgact ccctttcaga cgaacgcggg cttgcccaaa
      721 gcgatcccac aggacaaatg ggaggcgcag cggcgagtgg cggggagtcg tgcctaccag
      781 tattatcgga agtacgggaa atcggcacgg gttgctcccg aggatcggca gtagatgcaa
      841 ccgatgcgac cctcacctcc cgcatgacga ttctcaggtc gtagacaccc gcccgcgcaa
      901 taacctccgg actcccgaaa atctcctgca tatttcctcg cccctgtccc tcggatgggt
      961 tccgccgcgc tccgccggag ccttgacaat ccatcagtgt tgggatacac acgcaggtgt
     1021 tcctctgcgg caggaggggg atgttgccgc cgcgcagcga catgccgagc gtagcgagtg
     1081 gcggagggtg aatggctctc cggatctcat gggacctggg gagccctttg tgtttccatc
     1141 ccgaggtcgg gcgagagaaa acgtgtgatc gagtggctga ccgccagggg atgggtgagt
     1201 tccgtcgttg agtgcggcac ggtatggaga ttccggcgat ccttcggccc cgttgactgt
     1261 ggcaggggag caggccagtg aagcttaagg acagggtggc gctgatcacg gggagcagca
     1321 ccggaatcgg ccgggcgacc gctctggcgc tggctcggga aggcgcggat gtggtggtga
     1381 cctatatcac gaaccgggac aaggcggagg aggtggtcga agatatccag accatgggcc
     1441 gacgggcggt ctgccgacgg gtggacgtga ccaaggcgcc ggagatcgag cggttggtca
     1501 agggagcgct gcgtgccttc ggccgggtgg acatcctggt gaacaacgtg gggggcatcg
     1561 tccgccgctc ccccatcctg gcgatgagcg agcgggcttg ggatctgacc gtgaccctga
     1621 acttgaagag cacgttcctg tgctcccggg ccgtgatccc cttcatgatc aagcagaagt
     1681 gggggcggat catcaacatg tcctcgatcg cagcacgcct gggcggagcg gggggactgt
     1741 atccgtacgc cgtggcgaag gccggggtca tcgccctgac gaaggggctc gcccgggagt
     1801 tggcgccggc cgggatcacg gtgaacgcca tcgccccggg cgccattgac acgccctttt
     1861 actcccgagt ctccccgctc ttcgatctgc gtcagatcct tccaaacatc cccgtgggac
     1921 gcgtgggcac cgccgaggag gtcgccaagg tcatcgcgtt cttggcgtcg gacgaggcca
     1981 gttacatcat cgggcagacc atcgatgtga acggcggctg gattctgccg tgagagaggg
     2041 ggaggggtgc gggacccctc tccgacgtcg gaaacaccaa cgcaggatgg cacccgattt
     2101 gtcactcccg caacagaaag gagccgacca tggccgcgaa gaagatcctg atgctcgtgg
     2161 gtgactacgt cgaggactac gaggtgatgg tgccgttcca ggcgttgcag atggtggggc
     2221 acacagtcca cgcggtctgc gccggcaaga aatccgggga gagggtccgg acggcggtgc
     2281 acgatttcga gggggatcag acctacagcg aaaaaccggg gcacagcttc accctgaacg
     2341 ccagcttcga tcaggtgagg ccggagacct acgatgcgct ggtcatcccc ggaggacggg
     2401 cgcccgagta catccgcttg aaccaggatg tcctgaaggc tgtgcggcac ttcgctgaag
     2461 ccaacaagcc cattgcatcc atctgccatg gggcccagct cctggcggcc gccggcgtga
     2521 tcgagggaaa gcgctgctcc gcgtacccgg ctgtaggccc cgaggtgacg cgggccggcg
     2581 gcaagttcat ggagattccc gtggacgccg cccacgtcga cgggaacctc gtcaccgccc
     2641 cggcctggcc ggcccatccc gcctggctgg ccaagttcct ggaggtcctg gggacgaaga
     2701 tcacgctgtg acctggcgtc cagggcggac atcggagacc atggggcaga gaatggaggc
     2761 tatggatgtt ctgagggggc ggatccaaga gctggcgggg gccgccgctt tcgaggttga
     2821 gccccaggac gcagcggcgg ctccgggcac aaggcaacgg gggtgatatg gcggcgccac
     2881 gggaacaatg gttgcggttg acggcggagg accctctgga gccggccctt cccatttgcg
     2941 atccccatca ccatctgtgg gatcgcgccg aggagcggta tctccttgac gagctgcttc
     3001 aggacgtccg gagcgggcac aacatcgtct ccacggtctt catcgagtgc cgctccatgt
     3061 atcgcacgga cggccccgag gagatgaagc cgatcgggga gaccgagttc gtagagggca
     3121 tcaccgccca aagcgccagc gggcggtccg ggccgacacg tgcggcggcg gggattgtcg
     3181 gctacgccaa tctcaccctg ggggcagccg tggcggcggt gttggagggc caccgggcgg
     3241 ccagcccgaa ccgcctccgg ggcattcggc acatctgtgt gtgggatacg a
//
LOCUS       DBNGZC010000599         2408 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1322218, whole genome shotgun sequence.
ACCESSION   DBNGZC010000599 DBNGZC010000000
VERSION     DBNGZC010000599.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1322218"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..2031)
                     /locus_tag="AC16G0_10420"
     CDS             complement(103..2031)
                     /locus_tag="AC16G0_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5100062.1"
                     /translation="MKRAIWACLAGALLLLCPPAHAAVVEEPPSLAGMVAAGKLPPMV
                     ERLPEHPLVIETDPEAVYGGDLHMLVGTPKDLKLASVYGYARLVRFDRQYRIIPDIAE
                     RVEVEGGRVFTFHLRKGHRWSDGAPFTGADFAYWWEYVAKNRELSPAGPPPQLMVDGE
                     YPKVEFPDPWTVRYRWSKPNNLFLLDQASAAPTVLYRPAHYLKQFHIAFTNPEKLAKL
                     VKAERRRSWAGLHNARDSMYVMDNPDLPTLQPWRPTVSPPAEVFIAERNPYFHRVDQR
                     GRQLPYLGRIKMVLVDKSVIPIKTSTGEADLQARYLSFDDYTFLKKNERLGRYHVLLW
                     PTAATGVVVLYPNLTTSDPVMRKLLQDRRFRRALSLAINREEINKTLYYGLGVIGQNT
                     VLPGFGLGPKGELRMAYAQFDLAQADRLLDEMGLTQRDAKGYRLRPDGKRLDIILESS
                     GESTEQTDVLELVKDTWAEIGIELLTRPSQRTVYRRRVFSGEAMMSVGVGSGEFGLPT
                     PDMSPGWLAPVSEEQLQWSQWGLYFESKGQRGQPPTLPAAQKLAELYRRWLVSTDEAE
                     RARIWGEMLVINANEVFTIGILGDTPQPVVVANRLRGVPEKAVYTWDPGAQFGYLSPD
                     TFYWQGGRREPESMTKSQ"
     gene            complement(2028..>2408)
                     /locus_tag="AC16G0_10425"
     CDS             complement(2028..>2408)
                     /locus_tag="AC16G0_10425"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR5100063.1"
                     /translation="LVVVAYMADRIAVMFAGRIVELAPKRAIFEEPRHPYTETLLESV
                     LRPDPALRSSHPAPPQNGQSEALPAQGCPFALRCRYAVERCRVETPELTAVGPGHLAR
                     CHRVNDIHLTPLLPTSTGSLGSRP"
BASE COUNT          421 a          771 c          802 g          414 t
ORIGIN      
        1 gcaacgtaat tcagcatggg aagaatcctc gtcacgtgcc aaggacaaat gaccaatgac
       61 gcatgaccaa caattcccaa cggggcccct gtcgagcatt ggtcattggg atttggtcat
      121 cgattcgggt tcgcgtcgac cgccctgcca gtaaaaggta tccgggctca ggtagccgaa
      181 ttgagcccct gggtcccagg tgtaaaccgc cttctccggc acgccgcgca accggttggc
      241 caccacaacc ggttgcgggg tgtctcccag gatgccgatg gtgaacacct cattcgcatt
      301 gatcaccagc atctcgcccc agatgcgggc tcgctcggcc tcgtctgtgc tcaccagcca
      361 gcgtcggtaa agttccgcca gcttctgggc cgcgggcagg gtcggcggct gaccccgctg
      421 gcccttggat tcgaagtaga gcccccactg gctccactga agttgctcct cgctcaccgg
      481 ggcgagccaa ccggggctca tgtccggcgt gggcaggccg aattcgccgc tccccacccc
      541 cacgctcatc atggcttccc cggagaatac gcgacgacga taaaccgtgc gctggctcgg
      601 ccgggtgagc agctcgatgc cgatctccgc ccacgtatcc ttcaccagct ccagcacgtc
      661 ggtttgctcg gtggattcgc ccgacgactc caggatgatg tccaggcgct ttccatcggg
      721 tcgaagtcga tagcccttgg cgtcccgctg cgtgagaccc atctcgtcca gcaggcggtc
      781 ggcctgggcc aggtcgaact gggcgtaggc cattcgcagc tcccccttcg gccccaggcc
      841 gaagccgggg agcaccgtgt tctggccgat gaccccgagg ccgtagtaca gggtcttgtt
      901 gatctcctcg cggttgatgg ccaaggacag ggcccgacga aacctgcgat cctggaggag
      961 cttccgcatg accggatcgc tggtggtgag gttggggtac aggaccacga ccccggtagc
     1021 agcggtgggc cagaggagca cgtggtatct gccgagccgc tcgttcttct tgaggaacgt
     1081 gtagtcgtcg aagctgagat agcgcgcctg aaggtcggcc tcgcccgtgg aggtcttgat
     1141 ggggatcacg gatttgtcca ccagcaccat ctttatgcgg ccgaggtacg ggagctgacg
     1201 cccacgctgg tctacccggt ggaaataggg attccgctcg gcgatgaaga cctcggccgg
     1261 tggggagacc gtgggccgcc acggctgcag ggtcggcagg tccgggttgt ccatgacgta
     1321 catgctatcc cgagcgttat gcaggccagc ccaggaacgg cgccgctccg ccttgaccag
     1381 cttggccagc ttttccgggt tagtaaaggc gatgtggaac tgtttgagat aatgggccgg
     1441 ccggtagagc actgtggggg ccgcactggc ctgatccagg aggaacagat tgttgggctt
     1501 ggaccaacgg taccgcaccg tccagggatc ggggaattcc accttagggt attccccatc
     1561 caccatcaac tggggaggcg ggccggcggg ggaaagctcg cggttcttgg ccacgtactc
     1621 ccaccagtag gcgaaatcgg cgccggtaaa aggggccccg tccgaccagc ggtggccctt
     1681 tcggagatgg aaggtaaaga cccgccctcc ctccacctcc acccgttcgg cgatgtccgg
     1741 gatgatccgg tactgccgat cgaagcggac aagacgcgca tagccgtata ccgacgccag
     1801 cttcagatcc ttcggcgtcc cgaccaacat gtgcaggtcc cccccgtaga cggcctcggg
     1861 atccgtctct atcaccaggg gatgctcggg tagccgttcg accatggggg gcaacttccc
     1921 ggccgccacc atgccggcca gcgagggcgg ctcctcgacc acggccgcgt gggccggtgg
     1981 gcagagcagc aggagggccc cggccaggca agcccagatc gcgcgtttca tggacggctc
     2041 cccaggctgc ccgtggaagt ggggaggagc ggggtgaggt ggatatcatt cacccgatgg
     2101 cagcgcgcga gatgccccgg ccccaccgca gtcagctccg gggtctccac gcggcaccgc
     2161 tccacggcgt agcggcagcg cagggcaaag gggcatccct gggccgggag cgcctcagac
     2221 tggccgttct gcgggggggc gggatggctc gagcggagcg cggggtcggg ccggaggacg
     2281 gattccagca gggtctcggt gtacgggtgc cgcggctctt cgaagatcgc gcgcttgggg
     2341 gccaattcga cgatccgccc ggcgaacatg accgcgatgc gatcggccat gtacgcgacc
     2401 accacgag
//
LOCUS       DBNGZC010000600         3917 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2399708, whole genome shotgun sequence.
ACCESSION   DBNGZC010000600 DBNGZC010000000
VERSION     DBNGZC010000600.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2399708"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="AC16G0_10430"
     CDS             <1..643
                     /locus_tag="AC16G0_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015935335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIR5100064.1"
                     /translation="EDPRYITPDVYIVKVDDRFIVVLNDDGLPRLRISPYYRSLLANK
                     AGNGREVREYLEGRMRSALWLIRSIEQRQRTLFKVAESLVKFQREFLEKGITALRPLT
                     LKEVAEDIGMHESTVSRVTTNKYIHTPQGLFELKYFFHRGVPGTGGEAVSSLKVKDLI
                     HKLLTIEDSGKPLSDQKIVEVLRREHSIEIARRTVAKYRSQLRIPSSSRRKRY"
     gene            763..975
                     /gene="hpf"
                     /locus_tag="AC16G0_10435"
     CDS             763..975
                     /gene="hpf"
                     /locus_tag="AC16G0_10435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation-promoting factor, HPF/YfiA
                     family"
                     /protein_id="HIR5100065.1"
                     /translation="MQLSVKGRNLEVTDALRSYAEEKLRRLTKYFENIVTTNVVLSLE
                     KHRQIAEVTLRVRDLTIRAEESTEDL"
     gene            991..1284
                     /locus_tag="AC16G0_10440"
     CDS             991..1284
                     /locus_tag="AC16G0_10440"
                     /inference="COORDINATES: protein motif:HMM:NF027645.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54 modulation/S30EA ribosomal C-terminal
                     domain-containing protein"
                     /protein_id="HIR5100066.1"
                     /translation="MVEKLERQILRYKERIAAHVTRAASRGERGASATAEGESRLVKT
                     KRFAVKPAEVDEAIMQMDLLGHNFYVFRNARTDEVNVVYRRRDGHYGLIEPEG"
     gene            1287..1871
                     /locus_tag="AC16G0_10445"
     CDS             1287..1871
                     /locus_tag="AC16G0_10445"
                     /inference="COORDINATES: protein motif:HMM:NF019118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100067.1"
                     /translation="MDAETATPETTLPGDLVDVHGIGVLLLGESGTGKSECALDLITR
                     GHRLVADDVVEVTKDGERILMGRGPARIRHLMEIRGLGILSIRDLFGVAAIRYRKRIE
                     LVVMLEPWCPEEEYDRLGLDEQTYSILGVDIPMLRIPVSAGRNIAILVEVAARNHLLK
                     LLGLHVAQRFAKGLMASQGSQDADLPGFSREDTE"
     gene            1926..2792
                     /gene="rapZ"
                     /locus_tag="AC16G0_10450"
     CDS             1926..2792
                     /gene="rapZ"
                     /locus_tag="AC16G0_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIR5100068.1"
                     /translation="MAGIDFIVITGMSGAGKSAASRCLEDLGFFCIDNLPAALIPKVA
                     ELCAQSEKRIDRVALVIDAREGRFLDVLFDILGDLRRDGHQVRMVFLEASDEALVRRF
                     SESRRPHPLAPGGSALEGIQAERGRLAPLKAKADLVIDTSTFTVHEFRKLLGTSFLDL
                     PAPPRTCLSLVSFGYKHGLPVDADLMFDARCLPNPHFVETLQPLTGKAPQVVDYVLGF
                     AQARGFVDQILSFLTAMLPLYLQEGKAYLTIAIGCTGGRHRSVVLVEELARRLRDAGH
                     EVSVRHRDMERV"
     gene            2797..3222
                     /locus_tag="AC16G0_10455"
     CDS             2797..3222
                     /locus_tag="AC16G0_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012524234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR5100069.1"
                     /translation="MIGIVIVTHGDLGAELLRTAQEIVGKLPRVEAVSIHAAEQIEKA
                     RKKIVAVLQRVGDGGGVLILTDLFGGTPSNLVLSYLEEGKLEVVTGVNLPMLMKLPTL
                     REERDLRTLADKLARYGQRNILVASEFLAKQGKPAAREE"
     gene            3271..>3917
                     /locus_tag="AC16G0_10460"
     CDS             3271..>3917
                     /locus_tag="AC16G0_10460"
                     /inference="COORDINATES: protein motif:HMM:NF015052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 N-terminal
                     domain-containing protein"
                     /protein_id="HIR5100070.1"
                     /translation="MDGIRFILGIHAHQPVGNLPEVFAEAHRRSYLPFIEILSEFPQI
                     PFSLHISGVLLDWLEETHPAHLDRLAALVARGQVELLTGAYYEPILAAIPDADKLGQI
                     RRMTTYLRRRFGVTPKGMWLAERVWEPHLAKPIAEAGVEYVILDDNHFKAAGLPDSAL
                     TGRFLTEEQGATLALFPISQRLRYLIPFKEPAATVAYLRERAALAPGGLAIMADDA"
BASE COUNT          683 a         1279 c         1325 g          630 t
ORIGIN      
        1 ggaggatccg cgctacatca cgccggacgt ttacatcgtc aaggtggacg accggttcat
       61 cgtggtgtta aacgacgatg ggctcccgcg cctgcggatc agcccctatt accggtcgct
      121 cctggccaac aaggcgggca acggccgaga ggtccgcgaa tacctcgagg gccggatgcg
      181 ctcggccctc tggctgatcc gcagcatcga gcagcggcag cgaacgctct tcaaggtggc
      241 ggagagcctg gtgaagttcc agcgcgagtt cctggagaag ggcatcaccg ccctgcgacc
      301 gctgaccctc aaggaggtgg ccgaggacat cggaatgcac gagtccacgg tcagccgggt
      361 caccaccaac aagtacatcc acacccccca ggggttgttc gagctgaaat acttctttca
      421 ccggggcgtg cccggcaccg gcggcgaggc ggtgtcctcc ctgaaggtga aggacctcat
      481 ccacaagctg ctcaccattg aagacagcgg caagcccctc tccgaccaga agatcgtgga
      541 ggtgctgcgg cgcgagcact cgatcgagat tgcccggcgg accgtggcaa agtatcggag
      601 ccagcttcgg attccgtcct ccagtcggcg caagcggtac tgagtccggc tccgaggtcg
      661 ctgcacctcg cctccgggac gggcggtcgc tgccggggac gcaggagggt ccggacgacg
      721 gtccgcctta ccccatcccc aatcttcgga aggagcccga cgatgcaact ctctgtcaag
      781 ggtcggaacc tcgaggtgac ggacgccctc cgatcgtatg ccgaagagaa gctccggcgg
      841 ctcaccaagt acttcgagaa catcgtgacg accaacgtgg tcctgtccct ggaaaagcac
      901 cggcagatcg cggaggtgac cctacgcgtg cgggacctga ccatccgggc agaggaaagc
      961 actgaggacc tgtagtcctc catcgatctc gtggtggaga agctggagcg gcagatcctt
     1021 cgctacaagg agcggatcgc ggcgcacgtg acgcgcgcgg cctcccgggg ggagcgaggc
     1081 gcctcggcca ccgccgaggg ggagtcgcgg ctcgtgaaga ccaagcgctt tgccgtcaag
     1141 ccggccgagg tggacgaggc gatcatgcag atggatctcc tgggccacaa cttctatgtg
     1201 ttccgcaacg cccggaccga cgaggtgaac gtggtgtacc ggcgccggga cggccactac
     1261 ggtctgatcg agcccgaggg ctgagcatgg atgccgagac ggccaccccc gagaccacgc
     1321 tccccgggga tttggtggac gtccacggga tcggggtgct gctcctgggg gagagcggga
     1381 ccgggaagag tgagtgcgcc ctggacctca tcacccgggg tcaccggctc gtggccgacg
     1441 acgtggtgga agtcaccaag gatggcgagc ggatcctcat gggtcgcggc cccgcccgga
     1501 tccggcacct catggagatc cgggggctgg gcatcctcag catccgcgac ctcttcggcg
     1561 tggcggccat ccgctaccgc aagcggatcg agctggtggt catgctggag ccctggtgtc
     1621 ccgaggagga gtacgatcga ctcggtctgg acgagcagac ctactccatc ctgggggtgg
     1681 acatccccat gctgcggatc ccggtgtcgg cgggacgcaa catcgccatt ctggtggagg
     1741 tggcggcgcg caaccacctg ctcaagctcc tggggttgca cgtggcccag cgttttgcca
     1801 aggggttgat ggcgagccag gggagccagg acgcggatct ccccggcttc agtcgcgagg
     1861 acacggagta gccgtacccg ggggcgggtc cccacaggct cggcgaacca gggcagggca
     1921 agcgcatggc cgggattgat ttcatcgtca tcacggggat gtcgggggcg ggaaagagcg
     1981 cagccagccg atgcctggaa gacctggggt tcttctgcat cgacaatctt ccggctgccc
     2041 tgattcccaa ggtggcggag ctgtgcgccc agtccgaaaa acgtatcgat cgggtagccc
     2101 tggtgatcga cgcccgtgag ggacgtttcc tggacgtcct cttcgacatc ctgggagatc
     2161 tgcggcggga cggccatcaa gtgcggatgg tcttcctcga ggccagcgac gaggccttgg
     2221 tgcgccgctt cagcgagagc cggcgcccgc atccgctcgc ccctggcggc tcggcgctgg
     2281 agggcatcca ggcggagcgg ggccgtctcg ccccgctcaa ggccaaagcc gacctggtca
     2341 tcgacacctc gaccttcacg gtgcacgagt tccggaagct cctgggcacc tccttcctgg
     2401 atctgccggc gccgccacgg acctgcctct ccctggtctc cttcggctac aagcatgggc
     2461 tgccggtgga cgcggatctg atgttcgacg cccggtgcct gcccaatccc cactttgtgg
     2521 agacgctgca gccactgacg ggcaaagcgc cgcaggtggt agattacgtc ctgggcttcg
     2581 cccaggcccg aggctttgta gaccagatcc taagcttcct caccgcgatg ctccccctct
     2641 acctgcagga ggggaaggcg tatctcacca tcgcgatcgg ctgcaccggg gggcgccatc
     2701 gatcggtggt gctggtggag gaactggccc ggcggctgcg ggacgcgggc cacgaggtgt
     2761 ccgtccgcca ccgcgatatg gagcgagtct gacaccatga tcggcatcgt catcgtgact
     2821 cacggcgacc tgggggcgga gctcctgcgg acggcgcagg agatcgtggg aaagctcccc
     2881 cgcgtggaag ccgtctccat ccacgccgcg gagcagatcg agaaggcgcg gaagaagatc
     2941 gtggcggtac tccaacgggt gggcgacggg ggcggggtgt tgatcctcac cgacctcttc
     3001 ggcggtacgc cgtccaatct ggttctctcg tacctggagg agggcaaact ggaggtcgtc
     3061 accggggtga atctgcccat gctgatgaaa ctccccacgc tccgggagga gcgggacctc
     3121 cgcaccctcg cggacaagct ggcccgatac ggccagcgga atatcctggt ggccagcgag
     3181 ttcctggcca aacaggggaa gcccgccgcc cgcgaggagt gagcctcgga ccggggcctg
     3241 ggccatgacc cgaagtcgga gcaagcaccc atggacggca tccggttcat cctgggaatc
     3301 cacgcgcacc agcccgtggg gaatctccct gaagtctttg cggaggccca ccggcgttcc
     3361 tatcttccct tcatcgagat cctgagcgag ttcccgcaaa tccccttcag cctccacatt
     3421 tcgggcgtcc tcctggattg gctggaagag acgcatcccg cccacctgga ccgcctggca
     3481 gccctggtgg cccgaggtca ggtcgagctc ctgaccgggg catactacga gccgatcctg
     3541 gccgccattc cggatgcgga caagctggga cagatccggc gcatgacgac ctacctgcgc
     3601 cggcgcttcg gggtgacgcc caagggcatg tggctcgccg agcgcgtgtg ggagccccac
     3661 ctggccaagc cgatcgccga ggcgggcgtc gagtacgtga tcctggacga caatcacttc
     3721 aaggcggcag gcctcccgga ttcggccctc accggccgct tcctcaccga ggagcagggg
     3781 gcgaccctgg ccctgtttcc catcagccag cggctgcgct acctgatccc gttcaaggag
     3841 ccggcggcga cggtggccta tctccgcgaa cgcgcggccc tcgcgcccgg cgggcttgcg
     3901 atcatggccg acgacgc
//
LOCUS       DBNGZC010000601         1654 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_52915, whole genome shotgun sequence.
ACCESSION   DBNGZC010000601 DBNGZC010000000
VERSION     DBNGZC010000601.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_52915"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="AC16G0_10465"
     CDS             complement(<1..497)
                     /locus_tag="AC16G0_10465"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR5100071.1"
                     /translation="MDATPMSANLLPRRWRGGLLIACVAIIAAGCAVNPVSGRPEMAV
                     LSADKERELGVEEAKKVEASMGLVTEPALLAYVQAVGNRIARQSPRQDTSYAFQVVNR
                     PEPNAFALPNGNIYISRGLLALANSEDELAGVLGHEVVHVAARHTVRSANVAVATSPL
                     RIVTG"
     gene            complement(916..>1654)
                     /locus_tag="AC16G0_10470"
     CDS             complement(916..>1654)
                     /locus_tag="AC16G0_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR5100072.1"
                     /translation="RVAPVLDRILAKKELVVGTAANMPPLNRTLKDGKIVGMEMDIAA
                     QMATEIGVKLTVKPMNFNDLLPALEAGTVDLILSGMTMTPLRNIRVAFAGPYFGSGKS
                     VLIRQENMASLQSTEMMDSPDVTVTALKGSTSQLFVERFAPKAKLVLADDYDGAIALV
                     LDGKVRAMIADFPICNVSVYRYADKGLTTLKSPLNYEPIGIALSPTDPLLLNWAQNFV
                     DYLINSGELNSLRRKWFEDASWVPQLR"
BASE COUNT          275 a          569 c          510 g          300 t
ORIGIN      
        1 atccccgtga cgatccggag aggcgacgtc gccacggcca cgttcgcgct gcgcacggtg
       61 tggcgggccg ccacgtggac gacctcgtgc cccaggacgc ccgccagctc gtcttcggag
      121 ttcgccaggg ccagcagccc ccgcgagatg tagatgttcc cgttggggag cgcgaaggcg
      181 ttcggctccg gtcggttgac gacctggaag gcgtaagagg tatcctggcg cggcgattgc
      241 ctggcgatcc ggtttccgac cgcctgcacg tacgccagca gcgcgggctc agtcacgagt
      301 cccatgctcg cctcgacctt cttggcctcc tcgacgccca gctcgcgttc cttgtcggcc
      361 gagagcaccg ccatctccgg ccggccgctc accggattta cggcgcagcc ggcggcgatg
      421 atcgccacgc atgcgatcag cagccctccc cgccagcgtc tcggcaggag atttgccgac
      481 atcggagttg catccatgct cgtcctctcc cgttcagggg tcacaattca cgccggcaat
      541 ccgcctcccg cagccgttcg agatcctagt tgcgcagtcc ccagcgcgta gtgtactccc
      601 tttgggtgcg tgaatcgagg ttcggcagat tctctatcgt caccgggctc cccgcgatgg
      661 tgacgcccgg cagtcgggcc acgtcaggcg atcagtcgcg gccgctgcgt ggtgggatgg
      721 ccagcccaca gggaccacgg cgtccgaggc caagagcagc gtgaacgccc cgtgatggac
      781 tagccggttc ccggccacgg acctccgccg gggcgggggt gaagaggaag atcacgcgcc
      841 tgcatgtccg gatccaccgc catcagcctg atccctcagg attccccggc gccccgagca
      901 aaccctacgc cgctcttacc gaagctgggg aacccaggag gcgtcttcaa accacttccg
      961 ccggagcgaa ttcagctcgc cgctgttgat caggtaatcc acgaagttct gcgcccagtt
     1021 caggagcagc ggatcggtcg gggacagcgc gattccgatc ggctcgtagt tcagcgggct
     1081 cttcagggtt gtcagcccct tgtccgcgta gcggtagacc gacacattgc agatggggaa
     1141 atccgcgatc atggcccgga ccttcccgtc caggaccaac gcgatcgccc cgtcgtagtc
     1201 gtccgccaag actagcttcg ccttcggcgc gaaccgctcg acgaagagct ggctggtgga
     1261 gcccttgagg gccgtcaccg tcacatcggg gctgtccatc atttcggtgc tctggagcga
     1321 cgccatattc tcttgcctga tcaggacgga cttcccggag ccgaagtacg gcccggcgaa
     1381 agccacccga atattccgga ggggggtcat cgtcatcccg gacaggatca gatccaccgt
     1441 gccggcctcc agggcaggaa ggaggtcatt gaagttcatg ggcttcactg tcagcttgac
     1501 tccgatctcc gtcgccatct gagcggcgat atccatctcc atgccgacga tcttgccgtc
     1561 cttcaaggtc ctattcaggg gtggcatgtt tgcggcggtc cccaccacca gctccttctt
     1621 ggccaggatc cgatccagca ccggcgccac gcga
//
LOCUS       DBNGZC010000602         1342 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2596921, whole genome shotgun sequence.
ACCESSION   DBNGZC010000602 DBNGZC010000000
VERSION     DBNGZC010000602.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1342)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1342)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1342
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2596921"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..>1342
                     /locus_tag="AC16G0_10475"
     CDS             233..>1342
                     /locus_tag="AC16G0_10475"
                     /inference="COORDINATES: protein motif:HMM:NF012927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR5100073.1"
                     /translation="MASALEALFTPGTIGSMSVRNRIHMPPMGTNYATWTGAVTDRLI
                     TYHAERARGGVGLITVEYTYVHPTGRISYYALGVHDDALIPGLRRLTSAIHAEGAKVS
                     IQIAHAGRRAKSAITGTQPVAPSSIPCIGGEVPRELSPAEIQNVIAWFVQAARRVREA
                     GFDAVTLHLAAGYLLESFISPYANRRADRYGGSLEGRLRLPLEILERIRQDVGTEFPV
                     ICRLCVDEFVEGGLTLAEAQRMAQLLEGAGIAAIDTVAGIPETMYAVGPPMALPRGWL
                     VSHAQAIKEVVRIPVFAVGRINDPRLAEQILREGKADFISMGRPLLADPHLPNKAREG
                     RFGDICPCIACNEGCFQRLYNQLDISCVVNPRVGRE"
BASE COUNT          234 a          446 c          419 g          243 t
ORIGIN      
        1 atcgagccgt tcgcaggacc ggccgccctc cggacagcca gccctcattg gctggtcatg
       61 tgaacatccc caccgcacaa caaaacaaag actgattttg tcttgacact gccgtcaggg
      121 ctgcggcatg ctgccccgac tcgcacctcc gtcgtcacag gccaacaaag atttcctggg
      181 tgccgacaga caatacattc aagaaatttt cgcaccctca cgaggtattt tgatggcttc
      241 tgccctagag gctctgttca cccccggcac catcggcagc atgtctgtga ggaatcggat
      301 ccacatgccg cccatgggca cgaactacgc gacctggacg ggcgccgtga ccgatcgctt
      361 gatcacctat cacgccgaac gtgcgcgggg tggagtcggc ctcatcaccg tggagtacac
      421 ctacgttcat cccacggggc ggatttccta ctacgcgctg ggggtccacg atgatgcgct
      481 gattccaggg ctgcggcgtc tgaccagcgc catccatgcc gagggcgcca aggtgagcat
      541 ccagatcgcc catgccggtc gtcgagcaaa gagcgccatc acggggacgc agccggtggc
      601 gccctcctcc attccctgca tcggggggga agttccccgg gagctgtctc ccgccgagat
      661 ccagaatgtg atcgcctggt ttgtgcaggc ggcccggcgg gtgcgggagg ccggcttcga
      721 tgccgttacg ctccacctcg ccgccgggta tctgctggaa agcttcatct ccccctacgc
      781 caaccgacgg gccgaccggt acggcgggag tctggagggg cgcctccggc tgcccctgga
      841 gatcctggag cgcattcggc aagacgtggg aacggagttc ccggtcatct gtcgcttgtg
      901 cgtggatgag ttcgtggaag gcggcttgac gctcgccgag gcccagcgga tggcccaatt
      961 gctggaaggg gctgggatcg ctgcgatcga cactgtagcc ggcatccccg agaccatgta
     1021 cgccgtgggg ccgcccatgg ccctgccccg aggctggctg gtctcccacg cccaggcaat
     1081 caaagaggtg gtccgcatcc cggtctttgc ggtgggccgc atcaacgatc cgcggctggc
     1141 cgagcagatc ctccgggagg gcaaggccga tttcatctcc atgggccggc cccttctggc
     1201 cgatccccac ctcccgaaca aggctcggga gggacgcttc ggggacatct gcccgtgcat
     1261 tgcctgcaac gagggctgct tccagcgtct ctacaaccaa ctggatatca gttgcgtcgt
     1321 gaacccccgc gtggggcgcg ag
//
LOCUS       DBNGZC010000603         2053 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2204580, whole genome shotgun sequence.
ACCESSION   DBNGZC010000603 DBNGZC010000000
VERSION     DBNGZC010000603.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2204580"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="AC16G0_10480"
     CDS             complement(<1..1001)
                     /locus_tag="AC16G0_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012595408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR5100074.1"
                     /translation="MSGPRYPALYQINTRVWLTELSRSLGRPATLDDIPDAELDRVAK
                     MGFDWVWLLSVWRTGAAGQRVSRSNPEWRREFEQTLPDLREEDIAGSGFAITGYMVHP
                     GLGGDAALARLRQRLRQRGLRLMLDFVPNHTGPDHPWVKDHPEYYVPGTPEQLEREPQ
                     NYCRVELPGGPRVLAYGRDPYFSGWPDTLQLDYSNPATQEAMIGELLKIAGQCDGVRC
                     DMAMLVLPEVFDRTWGRRAPEFWPRATRRVRGQAPGFCFMAEVYWDLEWTMLQQGFDY
                     AYDKRLYDRLREGRARPVREHLYAGLDYQSKLARFLENHDEPRAAATFAPGMHEAAA"
     gene            complement(1105..2053)
                     /locus_tag="AC16G0_10485"
                     /pseudo
     CDS             complement(1105..2053)
                     /locus_tag="AC16G0_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770118.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="MGH1-like glycoside hydrolase domain-containing
                     protein"
BASE COUNT          384 a          712 c          591 g          366 t
ORIGIN      
        1 atggccgcgg cctcgtgcat gccgggcgca aaggtcgccg cggcccgggg ctcgtcatgg
       61 ttttccagga accgggcgag cttgctctgg tagtcgagcc ccgcgtacag gtgctcgcgc
      121 accggccgcg cgcgcccctc gcgcaggcgg tcgtagagcc gcttgtcgta ggcgtagtcg
      181 aacccctgct ggagcatcgt ccattccagg tcccagtaca cctcggccat gaagcagaag
      241 cccggggcct gcccccgcac gcgccgggtc gccctcggcc agaactctgg cgcccggcgg
      301 ccccaggtcc ggtcaaacac ctccggcagg acgagcatgg ccatgtcgca gcgcaccccg
      361 tcacactgtc ccgcgatctt cagcagctct ccgatcatgg cctcctgcgt ggccggattg
      421 ctgtaatcga gctggagcgt atccggccag ccggagaagt aggggtcgcg tccataggcc
      481 agcacgcgag gtcctccggg caactcgacg cgacagtagt tctgcggctc gcgctcgagc
      541 tgctcgggtg ttccggggac gtaatactcg gggtggtctt tcacccaggg gtgatccgga
      601 ccggtgtggt tggggacgaa gtcgagcatc agcctcagcc ctcgctgcct gaggcgctgg
      661 cgaagtcggg ccagcgccgc atcccctccc agaccgggat gcaccatgta gccggtgatg
      721 gcaaacccgg aaccggcgat atcctcctcg cgcagatccg gcagcgtctg ttcgaactcc
      781 cgtcgccatt cgggattgct gcgcgacacc cgctgtccgg ccgcccccgt ccgccacacg
      841 ctcagcagcc agacccagtc aaaccccatc ttggccacgc ggtccagctc ggcgtccgga
      901 atgtcatcca gcgtggccgg ccggcccaga gacctggaca actccgtcaa ccagacgcgc
      961 gtgttgatct ggtagagggc gggataacgc ggcccagaca tcacagccct ccttcgccac
     1021 tactttatat gaagatgttg acggtgccaa tccctcgatc actcgcggtt cacgccttat
     1081 cgggagtcga acttacccga cccctcacct gttgatctgt tctccgccca cctgctccct
     1141 tacaaccctg gccatcacgc gcccgcgttc ggtctccagc agggctttag cgtcgacacg
     1201 cccgaacaaa ttcatcaggg gggcgacgac accggtccac ccggtctggt ggctggcccc
     1261 gagccccgct ccattgtcgc catggaagta ctcaaaaaac aggatcaggt cgcgccaatg
     1321 cggatcttcc tggaacttgg ccgtcccccc gtagaccggc cgtcgtccgt tagcatcacg
     1381 caagaatgtt cccgccagcc ggcggacaag ttcctgcgcc acttcgaaca gtgtcatgta
     1441 acgccccgaa ccggtcgggc actgaacttt gaattcatcg ccgtaaaagc tgtagaggtt
     1501 taccagcgct cggacaatca gcgcattgac cggcatccac accggcccgc gccagttgga
     1561 gttgccgccg aacatccccg tattcgattc agccggcagg tactgcaccc tgaactcctg
     1621 atgacccacc cagaacgtga acggatgatc caggtgatac cgcgagagag aacgaattcc
     1681 gtgcggcccg agaaactcgt tctcgtcgag catgtaaccg agcacccgct ccaacttttg
     1741 cttgttgagg accgacagga gacgccgccc cttatgtccg atgaaaccct catcggtcgg
     1801 ggccacctgg gaaaccagct ccggatgacg tttccggtac agggcgatca tctccatgag
     1861 tttaggatgc cgcgtgacgt cctcggcctc gaacacggtc gatgcgcaca gcggcaacag
     1921 tcccaccagt gatcgcacct tcaaacgcac ggcctggccg tcagggagcc gcagcagatc
     1981 atagaaaaac ccgtcttctt aatcccacat ctcgtcgtga tgcatgccga tgcggtccat
     2041 ggcataggca atc
//
LOCUS       DBNGZC010000604         1640 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2303842, whole genome shotgun sequence.
ACCESSION   DBNGZC010000604 DBNGZC010000000
VERSION     DBNGZC010000604.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2303842"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="AC16G0_10490"
     CDS             <1..468
                     /locus_tag="AC16G0_10490"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5100075.1"
                     /translation="RDLPIQIHTGLQEGNENLLTNANPTHLANLFIEYREAKFDLFHG
                     GYPYLGEALALAKNFPNVFLDLCWLHIISPTAGARMLHEAIETVPANKIFAFGGDFII
                     PEGAYGHSVLARRVVSRVLTEKVAEAYLSEEEAIRLAQRILRDNPAALFRLKL"
     gene            511..1320
                     /locus_tag="AC16G0_10495"
     CDS             511..1320
                     /locus_tag="AC16G0_10495"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR5100076.1"
                     /translation="MSETGPGMRAADREIFISGVQFEGEFKSGVRTVLDVAPLAVKLG
                     VQGVEYRDVYWKDKPRELPAVRDQIARLKLKATYTTVTPLYSREPAMRQQLLQDIEDA
                     KTLGSPLLRVTLGESPGDGRDAAAIRTAARGAIEQAIMRGIRLSLENNSKAPGHTLEE
                     IKGALEEFKSPFLMTNIDFANFVTTGQDAVAAIKALAPHINYTHAKDARKTPDSWKAT
                     YLGNGSLPLQEIIAALDATGRRIPFCFEFPGEGDPESAIRKSQEFMARLGA"
     gene            complement(1493..>1640)
                     /locus_tag="AC16G0_10500"
     CDS             complement(1493..>1640)
                     /locus_tag="AC16G0_10500"
                     /inference="COORDINATES: protein motif:HMM:NF024833.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HIR5100077.1"
                     /translation="ACGTVTGAAIGAGSGAAIGAGPGYGAGKGALIGTGVGAAAGAVY
                     DVTH"
BASE COUNT          318 a          546 c          515 g          261 t
ORIGIN      
        1 cgcgacctcc ccatccagat ccataccgga ctgcaggaag gcaacgagaa cctgctcacg
       61 aatgcgaacc cgacacacct ggcgaacctg ttcatcgaat accgggaggc gaagttcgac
      121 ctcttccacg gcggatatcc ctatctgggc gaggcgctgg ccctggccaa gaacttcccg
      181 aatgtctttt tggacctctg ctggctccac atcatctcac ccaccgcggg cgcccggatg
      241 ctgcacgagg ccatcgagac ggtgcccgcc aacaagatct tcgccttcgg cggggacttc
      301 atcatcccgg agggcgccta cggtcacagc gtgctcgctc gccgggtggt gagccgggtc
      361 ctgacggaga aagtggcgga ggcatacctc agcgaggaag aggccatccg tcttgcccag
      421 cgcatcttgc gggataaccc ggcggcactc tttcgcctca agctctgagg cgaaattgcg
      481 acgtgaggtc caggcgagga gggccgtgct gtgagtgaga ccggtccagg catgcgggcg
      541 gcggatcgcg agatcttcat ctccggggtt caattcgagg gcgagttcaa gtcgggggtg
      601 aggacggtcc tggacgtggc gcccctggcc gtcaagctgg gtgtccaggg cgtagagtac
      661 cgggatgtct actggaagga caagcccagg gagttgcccg ccgtgcggga ccagatagcc
      721 aggctgaagc tgaaggcgac ctacaccacc gtgacgcccc tatacagcag ggagccggcg
      781 atgcggcagc agttattaca ggacatcgag gacgccaaga ccctgggctc tcccttgctg
      841 cgcgtcaccc tgggcgagtc accaggagac gggcgtgacg ccgcggcgat ccgaacggcg
      901 gcccgcggtg cgatcgagca ggcgatcatg cgcggaatcc gcctgtcgct ggagaacaac
      961 tccaaggccc ccggccacac actggaagag atcaagggcg cactggagga gttcaagtcc
     1021 cccttcctga tgaccaacat cgactttgcg aatttcgtga cgaccggcca ggatgcggtc
     1081 gccgcgatca aggccctggc accgcacatc aactacacgc acgccaagga tgccaggaag
     1141 actccggaca gctggaaggc gacctacctc gggaacggta gcctgcccct ccaggagatc
     1201 atcgccgccc tggacgcgac cggcaggcgg atccccttct gcttcgagtt ccccggggag
     1261 ggcgatcctg aaagcgcgat ccggaagagt caggagttca tggcaaggct cggagcgtga
     1321 ccagcacgag caggccctct tctgtgaatt ccggcctgcc agtattgtca tacgccgtgg
     1381 gctggctcta tgccaaggca gtcacgctca ggaggatgcc aaggctggcc acaattccca
     1441 cccacaggcg tatccaccat tttcgcattg gagcctccag cggctgacgc gcctaatgcg
     1501 tgacgtcata gaccgcgccc gcggccgcgc caacccctgt tccgatgagc gcacccttgc
     1561 ctgccccata ccccggtccc gcgccgatcg ccgcgccact ccccgcgccg atcgccgcgc
     1621 cggtgaccgt gccgcaggcc
//
LOCUS       DBNGZC010000605         1332 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1226942, whole genome shotgun sequence.
ACCESSION   DBNGZC010000605 DBNGZC010000000
VERSION     DBNGZC010000605.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1332)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1332)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1332
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1226942"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="AC16G0_10505"
     CDS             complement(<1..686)
                     /locus_tag="AC16G0_10505"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR5100078.1"
                     /translation="MKTESQAIKRLGASQRGLADRLPKPRVDKVIKLSSGDPSFPTPA
                     YIVEAAYRAMKDGYTHYPPVQGDPELREAIAAYQSHISGVPVSPAEVLVTSGGTGAIT
                     AAMMALLDEGDEVIILDPSYSLYADVARVVGAHVVFVPLTSGLGVDVEGVRGAVTPRT
                     RMLILNYPSNPTGQLLESQELDGLAAIAVEHDLAVLSDEVYDQLVFQGKHSSALGHPA
                     LTDRTVLVNA"
BASE COUNT          244 a          460 c          384 g          244 t
ORIGIN      
        1 aaggcgttga ccaggacagt cctgtcggta agggccggat gccccagcgc ggaggaatgc
       61 ttcccctgga acaccaattg gtcgtacacc tcatcggaga gcacagccaa atcgtgctcg
      121 acggcgatgg ccgccaggcc atcgagttcc tgcgactcca gcagttgccc agtcggattg
      181 gaggggtagt tcaggatcaa cattcgggta cggggtgtga cggccccccg cacgccctcc
      241 acgtccaccc cgagaccgct ggtgagaggc acaaagacca cgtgcgcccc caccaccctg
      301 gccacgtcgg cgtacagcga gtaggagggg tccaagatga tgacttcatc cccctcgtcc
      361 aggagagcca tcatcgccgc agtgatggct ccggtgcccc cgctggtcac caggacctct
      421 gcaggtgata ccggcacgcc gctgatgtga gattgatagg cggcgatggc ctcccggagc
      481 tctggatcgc cctggaccgg cgggtagtgg gtgtacccat ccttcatcgc acgataggca
      541 gcctcgacga tgtaggccgg cgtcgggaag ctggggtccc cgctgctcag cttgatgacc
      601 ttgtccactc tgggcttcgg cagccgatcc gccaggcccc gttgtgaagc ccccagtcgc
      661 ttgatagcct gtgactcagt tttcatcgcc atctactccc ttcgctacag ccggaatccc
      721 tataggcctg aaagctggcg gtgcctcggg gagaaatgca tggacgcctc ccctattgaa
      781 gggccatgcc cacctcgcaa agccgacggt ctgcgcggtc gcgccgccat caccatttgg
      841 agactatccg tgatgcttca cgagacgccc acgcgtcctt cctacaccga accggaggag
      901 cctgtaaagg acctccccga agttgtaggc aggaccacgc ataggtcggt ctgactgcgg
      961 cccaagcctc aatccttctg gtccccctgg agccctcgcc agtcgcccgt tgtggcattt
     1021 gccgttgaca ccccagggcg ccgaggctag gctctgccca cgcctcctgg gactcaccac
     1081 gctcagcggg aacgctgcat ccctcgagcc ttcgaggccc aatccggtct atcgaaccgg
     1141 tgatgcgggg ctcgctcttt gcatgcaccc atgacgaaga acgccgcccc actcagcatc
     1201 cgatcacctc gcggcgcatc cacccatgcg atttccagca gccctccgcg cactcgagca
     1261 tcgagactac cgcctgttct tttcaggcca actcatctcg ttgatcggca cctggatgca
     1321 gagggtggcg ca
//
LOCUS       DBNGZC010000606         4824 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1422915, whole genome shotgun sequence.
ACCESSION   DBNGZC010000606 DBNGZC010000000
VERSION     DBNGZC010000606.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1422915"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            480..1229
                     /locus_tag="AC16G0_10510"
     CDS             480..1229
                     /locus_tag="AC16G0_10510"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR5100079.1"
                     /translation="MPVLCLGHAQPALAGALERAMLGKAAIVDLTHVMGDHPRIPPGQ
                     EPSRQESGEGDRPSPGFRAAMPGDLDTHLDVPMAGQKIRPTVADISPRELLVRAVVVD
                     IAAKVAQGTEYRVSVVDLQSWERRNGRIPKQALVLLHTGWSKRWGNPEQYLNLDPQGV
                     PRVPGFSAAALAFLVNDRDVCGVGLDAFMSDGSQASGGGEARASLLAGKFQLENLTNL
                     DQLPTTGVKLMIMPLRVEGGSAPARVIAILP"
     gene            1341..1976
                     /locus_tag="AC16G0_10515"
     CDS             1341..1976
                     /locus_tag="AC16G0_10515"
                     /inference="COORDINATES: protein motif:HMM:NF015682.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HIR5100080.1"
                     /translation="MPDLLRRIARIPTAVLSDALGRLGTMASAIRPVTSSARLIGVAR
                     TVRCFPGDCLTLLRALAEAREGEVLVVDGGGMVEAAVMGRLMAIEARRKGLCGFIVDG
                     AVRDLHGLEAADLPVFARGVTPRMGTAEHLEETQVPIICGGAPVRPGDIVHGDLDGVV
                     VFGAKRLREVTARALEIERHETDVVRRLKRGQPLAELTKLFDRLAELRKRG"
     gene            1979..3163
                     /locus_tag="AC16G0_10520"
     CDS             1979..3163
                     /locus_tag="AC16G0_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR5100081.1"
                     /translation="MSGPLVGVRVLDLTRVLAGPFTTMLLADLGAEVVKVEEPGVGDE
                     ARRIGPFLKGVSTYFISINRGKKGLTLNLKNQTGRRLFLDLVGQADVLVENYRPGVMK
                     KLGLDYPVLHTRCPHLVYAACSGFGQTGPYAERPAYDMIVQGMGGIMSITGEMGGSPV
                     RVGVSIGDLGAALFTTVGILAAICHARATGEGQLVDVGMLDCQVALLENAIMRNLVTG
                     EVPRPLGSRHPSIAPFEAFPAADGQVILAVGTKQWARFCQAINRPDLIQHPHFSTNAL
                     RADHVDELREILAPITRQKAVAVWIQEMEAIGVPCGPVNTVEQTVKDPHVQAREMILK
                     TLDPEAGPVSMAGLPIKLSATPGAIQGPAPRLGEHTETVLAEWLHLQPSAVARLREEG
                     VV"
     gene            3165..3566
                     /locus_tag="AC16G0_10525"
     CDS             3165..3566
                     /locus_tag="AC16G0_10525"
                     /inference="COORDINATES: protein motif:HMM:NF017876.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HIR5100082.1"
                     /translation="MNTLGISALVVMALVSLLWTALLLPVLFEMRRASWRLQEFIRSV
                     EMELRPLIQEARQALKNVEHAAEGVAGGVTHLRGAFSAIEEAGENLRATTGVIRAVFG
                     SRLIPAATLIAGLRAGLRMLWKRSPRRRETT"
     gene            3563..3874
                     /locus_tag="AC16G0_10530"
     CDS             3563..3874
                     /locus_tag="AC16G0_10530"
                     /inference="COORDINATES: protein motif:HMM:NF024143.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HIR5100083.1"
                     /translation="MSEDRGYSGAAVALGFILGGALGACLAVLFAPESGRRTRERLRD
                     LAADVRDKTIDLSEDFRDKAEDVVERGREVLEEKKSILSAAVQAGREAMQRERDRLSP
                     H"
     gene            4026..>4824
                     /locus_tag="AC16G0_10535"
     CDS             4026..>4824
                     /locus_tag="AC16G0_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR5100084.1"
                     /translation="MKTIILQEPGRFQLSDTPSPASPGPGEALVRIHRVGVCGSDIHA
                     FKGNQPFFSYPRVVGHELGIEIVAVGPNDKGLTAGERCAVEPYLNCGRCIACRRGKTN
                     CCVNLQVLGVHTDGGMREFMTVPVGKLHRSRTLSVDQLALVEMLTIGAHAVRRAQLEP
                     GENVLVIGAGPIGLATLQFAHLVGARVGVLEISQWRREFCRRHLGVNRCLDGTGDTGR
                     QLEGFFNGDLPTAVFDATGSPQSMMQAVQYVAHGGKLVYVSLVQADLT"
BASE COUNT          765 a         1553 c         1716 g          790 t
ORIGIN      
        1 gaactcctgc tggaagcaat aggggcaaaa cctcatgtcc cgggggagct gctgttcctc
       61 gaccatgacg acgcctcctg gatgggcccc ctccgcagga tctcagctta accgaggtac
      121 cccggaacgt caatccgctc ggcttcccgc catcccgcag gctgtagtcc gccgccgcgc
      181 ggcacggaaa atccaccggt gattgcccag caggtaaaac cttcgagcgc ctcccagggg
      241 agagctagcc cttccccacg ggtcgtcatg ggaaggcaga tccgcggtgt gtggggcctg
      301 gcagccgcct gcctgcgcgt tgacagccat ttgggcctat ggtatggtcc gtttcgtcaa
      361 cttcgcgcac gccgaaattg cgcgagcgga ggagtgtcgt cagggctcgg ccctgacttt
      421 ttgttcaggg tacgcgaatg gggcattcgc tccgagtgca aggtccgata tgggcgctgg
      481 tgcctgtcct gtgcctgggg catgctcagc cggccctggc gggagcgctg gagcgggcca
      541 tgctcggcaa ggcggccatc gtggacctga cgcacgtcat gggggaccac ccgcgcattc
      601 ccccaggcca ggaaccgtcc agacaggagt cgggggaagg ggaccgtcct tcacccgggt
      661 tccgcgccgc gatgccggga gatctggaca cgcacctgga cgtgccgatg gccggccaga
      721 agatcaggcc gaccgtggcg gatatttcgc cgcgtgagct cctggttcga gcggtcgtcg
      781 tggacatcgc cgccaaggtc gcccagggaa cggaataccg tgtgtcggtg gtggacctgc
      841 agtcctggga gcgccggaat gggcgcattc ccaagcaggc cctggtcctg ctccacaccg
      901 gctggtccaa acggtggggc aaccctgaac agtatctgaa tctggatcct caaggcgtcc
      961 cgcgggtgcc cggattctcc gcggcggccc tggctttcct cgtgaacgac cgggacgtct
     1021 gtggcgtagg cctggatgcc ttcatgtctg acggatctca ggcgagcggc ggcggtgagg
     1081 cacgcgccag tttgctggct ggcaagttcc agctcgagaa tctcacgaac ctggatcagc
     1141 ttcccacgac gggggtcaag ctgatgatca tgccgctccg ggttgaaggc ggctccgccc
     1201 ccgcgcgggt gatcgccatt ctgccgtgaa tcctcaaccg cccgccgtga cccgagctgc
     1261 cgcagacccc cggcgccgac cgggaatgcg cccgggttgt ctgcgccccg cgggggactc
     1321 gcggcgctcg aaggccgacc gtgcctgacc tcctccggcg tatcgcccgc atcccgaccg
     1381 cggtgctgtc ggatgccctg ggccggttgg gaaccatggc ctcggccatc aggccagtga
     1441 catcctccgc gcggttgatc ggggtcgcgc ggacggtgcg ctgctttccg ggggattgcc
     1501 tgacgcttct ccgggcgctg gccgaggcgc gggagggcga ggtcctggtg gtggacggcg
     1561 gcgggatggt ggaggccgcg gtcatgggga ggctcatggc catcgaggcg cggcgaaagg
     1621 gtctctgtgg cttcatcgtg gacggggccg tccgcgacct tcacggcctg gaagcggcag
     1681 atcttcccgt ctttgcgcgt ggagtcacgc ctcgcatggg gaccgcggag cacctggagg
     1741 agacgcaggt gcccatcatc tgcggaggcg ctccggtgcg acccggcgac atcgtccacg
     1801 gagacctgga cggcgtcgtg gtcttcggcg cgaaaaggct gcgagaggtg acggcgcgcg
     1861 ccctggagat cgaacggcac gaaaccgacg tggtgcgccg gctgaagcgg ggccagccgc
     1921 tggcggagct gacgaaactc ttcgacagac tggccgagct gcgaaaacga ggttgagcat
     1981 gtccggtccc ctggtgggcg tgcgcgtcct ggacctgacg cgggttctcg ccggtccctt
     2041 cacaaccatg ctcctggccg acctgggcgc cgaggtcgtc aaggtcgagg agccgggggt
     2101 gggggacgag gcccggcgga tcgggccatt cctcaaaggg gtgagcacct acttcatcag
     2161 catcaaccgg ggcaagaagg ggttgacgct gaacctcaag aaccagacgg gccgtcggct
     2221 gttcctggat ctggtcggtc aggccgatgt gctggtcgag aactaccggc cgggcgtcat
     2281 gaagaagctt ggcctggact acccggtcct ccatacccga tgcccgcacc tggtgtacgc
     2341 cgcatgttcc ggcttcggcc agaccggacc ctacgcggag cggccggcct acgacatgat
     2401 cgtccagggg atgggtggca tcatgagcat cacgggagag atgggcggct cccccgtgcg
     2461 cgtgggggtg tccatcggcg atctcggggc ggcgctcttc acgaccgtcg gcatcctggc
     2521 tgccatctgt cacgcccgtg ccaccggaga ggggcagctc gtggacgtcg gcatgctgga
     2581 ttgccaggtg gccctcttgg agaacgccat catgcggaac ctggtgaccg gcgaggtccc
     2641 gcggcccctg ggctcgcgcc atccctcgat cgctcccttc gaggcgttcc cggcggcgga
     2701 cggtcaggtc atcctggctg tggggaccaa gcagtgggct cgcttctgcc aagccatcaa
     2761 ccggcccgac ttgatccagc acccgcattt cagcaccaac gcgctccggg cggatcatgt
     2821 ggatgagctc cgcgagatcc tggcccccat cacgcgtcag aaggcggtgg cagtctggat
     2881 tcaggagatg gaagccatcg gcgtcccctg cgggcccgtc aacacggtgg agcagacagt
     2941 caaggacccc cacgtccagg cgcgggagat gatcctcaag acgctggacc cggaggccgg
     3001 gccggtctcc atggccggtc tgcccatcaa gctgtccgcc accccaggtg cgatccaggg
     3061 gccggctccg cgcctggggg agcacaccga aacggtgctt gccgaatggc tgcacctgca
     3121 acccagcgcc gtggctcggc tgcgggagga aggggtcgtg tgagatgaac accctcggca
     3181 tctccgccct ggtggtcatg gccctcgtca gcctgctctg gaccgcgctg ctgctcccgg
     3241 tgctgttcga gatgcgccgg gcatcctggc gcctccagga attcatccgg tcggtggaga
     3301 tggaactgcg gcccctgata caggaggcgc ggcaagccct caagaacgtg gagcatgcgg
     3361 ccgagggcgt tgcgggaggg gtgacccacc tccgcggggc cttctcggcc atagaagagg
     3421 cgggagaaaa tctccgggca accactggcg tgatccgcgc cgtgttcggc tcgcgcctca
     3481 taccggcggc gaccctcata gcgggcctcc gagccggact gaggatgttg tggaagcggt
     3541 ccccacgaag gagggagacg acatgagcga agatcgaggg tactccgggg cggcggtcgc
     3601 gcttggattc atcttgggtg gcgccctggg ggcgtgtctg gccgtcctgt tcgcgcccga
     3661 atcgggccgg cggacgcgcg agcggctgcg ggacctggcg gctgatgtgc gggacaagac
     3721 catcgacctg tccgaggact tccgcgacaa ggccgaggac gtggtggagc gcggccgaga
     3781 ggtcctggag gagaagaaat ccatcctctc ggcggcggtg caggcgggcc gcgaggccat
     3841 gcagcgcgag cgggatcgcc tgtcccccca ctgaccggga tcgcgatatc cgctgatccg
     3901 tccggacgga agccgcgccc gacggagtgt cccggtggta ggctggggag gggtgtatcc
     3961 aacggcgtgt gtcccgaaat gccacgcggg ggtcgcagcg tgcacaagga gaacgggcag
     4021 ggcccatgaa aaccatcatc ctccaagagc cgggtcgctt tcagctcagc gacacgccgt
     4081 cgcctgcctc cccgggaccc ggtgaggccc tggtccgcat ccaccgcgtg ggtgtctgtg
     4141 gctccgacat tcacgccttc aagggaaacc agcccttctt cagctacccc cgtgtcgtgg
     4201 gacacgaact ggggatcgag atcgtggccg tcgggcccaa cgacaagggc ctcaccgccg
     4261 gcgagcgctg cgccgtggaa ccgtacctga actgcggtcg gtgcatcgcc tgccgtcgcg
     4321 gcaagacgaa ctgctgcgtg aacctccagg tcctgggcgt gcacaccgat ggcgggatgc
     4381 gggagttcat gaccgtcccg gtggggaaac ttcaccgctc ccgaacgctt tcggtggatc
     4441 aactcgccct ggtggagatg ctcaccatcg gggcccacgc cgtccgacgg gcccagttgg
     4501 agcccgggga gaacgtcctg gtgatcggcg ccgggccgat cggccttgcc actctgcagt
     4561 tcgcgcacct ggtgggcgcc cgggtcggcg tcctggagat cagccagtgg cggcgggagt
     4621 tctgtcggcg gcacctgggg gtgaaccgct gcctcgacgg gacgggggac acggggaggc
     4681 aactcgaagg cttcttcaac ggggacctgc ccacggcggt gttcgacgcc accgggagtc
     4741 cccagtcgat gatgcaggcg gtgcagtacg tggcccatgg ggggaagctg gtctacgtgt
     4801 ccctggtcca ggcggacctg acct
//
LOCUS       DBNGZC010000607         3068 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_150871, whole genome shotgun sequence.
ACCESSION   DBNGZC010000607 DBNGZC010000000
VERSION     DBNGZC010000607.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_150871"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="AC16G0_10540"
     CDS             <1..918
                     /locus_tag="AC16G0_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5100085.1"
                     /translation="RRLLQFIPTILAISLITFVLLNVLPGNAALMAAGAQDRGLDAKF
                     VEQMMKRWDLDKPLHVRYWMYLKNLARGDLGLSFLRREPVGEVILARLWPTLKLALAA
                     MLIAVGLGIPLGFVSALRRGSWLDAFSMIGAVSGVSLPQFWLGLLLMFLLSVKLRLLP
                     TSGYGEGGIAHLLLPAITLGVGYMALLARTTRAAVLEILSADFVRTAAAKGLSRLTVN
                     SKHVFRNALILVLTTAGLQFGSLMGQTVIVEKLFSWPGIGSLLVDTIFQRDIPVVQGC
                     VLLIILIFLVVNLAVDVLYALIDPRIEYQ"
     gene            957..1757
                     /locus_tag="AC16G0_10545"
     CDS             957..1757
                     /locus_tag="AC16G0_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019812834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5100086.1"
                     /translation="MIGGIITLAIVCVALLAPFLAPYDPINHADLLHSEEPPSRTFFF
                     GTDTQGRDILSRVIFGARISLSIGLITQSLNSLIGILLGLTAGFLGRWWDDLVMGLTN
                     VMLSIPALVLALAIMAVLGPGLFNVFLAIGFTNWSYSCRVTRSQVLATRSLDYVTAAR
                     ALGYGRVRIMLTQILPNIVGPILVIATLGVAYAILTEASLSFLGLGAQPPAPSWGGML
                     STAREQLFTAPWISIFPGLAIFVTVLGLNLLGDGLRDILDPHTLRQGR"
     gene            1798..>3068
                     /locus_tag="AC16G0_10550"
     CDS             1798..>3068
                     /locus_tag="AC16G0_10550"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR5100087.1"
                     /translation="MDLAILSSRILTGDPGRPWAEALCIKDDRVAAIGTNAEARHASG
                     PGTRIFELPDRLVTPGIVDAHLHFVNFGLYLQRVDLRDLTSIAACRERVKAAVGKKKP
                     GEWVIGRAWNEHIWSDRRQPNAKDLDDISPDHPVMLVRCCGHSVWLNGLAMKLAGITQ
                     ETVDPPAARIERDAGGNPIGLCHEYRKIIERIIPPPSLDERKAAALRAQAEALRHGVT
                     GVHSCETLQEWDALAALEAEGSLKVRVHHTLPPEEVATAHSRGIEPGKGSERLWFGQV
                     KLFADGSLGSGTALLHAPYADDPASTGLEVLSVAELQERIELAYRYGGDVAVHAIGDL
                     ATTNVLDAIEGARKKFHGPKRDRIEHVQLMHPSDFARFRVMEVVASCQPVHLHTDMPV
                     AEKKWGMDRCRYGYAWKSMLKEGIRLQFGSDA"
BASE COUNT          495 a         1000 c          991 g          582 t
ORIGIN      
        1 cgtcgcctgc tgcaattcat ccccaccatc ctggcgatct cgctgatcac cttcgtcctg
       61 ctcaacgtgc tccccggcaa cgccgccctc atggcggccg gagcgcagga ccgtggcctg
      121 gacgcgaagt tcgtcgagca gatgatgaag cgctgggatc tggataagcc cctgcacgtc
      181 cggtactgga tgtacctgaa gaacttggcc cgcggggatc tgggcctgtc cttcctgagg
      241 cgggagcccg tgggcgaggt gatcctggcc cgtctgtggc cgacgctgaa gctggccctg
      301 gcggccatgc tcatcgccgt ggggctggga atcccgttgg gcttcgtctc ggcattgcgt
      361 cggggctcgt ggctagacgc cttttccatg atcggggcgg tgtcaggcgt ctcgctgccg
      421 cagttctggc tcggactcct gctgatgttt ctcctgtcgg tgaagctgcg gctcctgcca
      481 acctcgggct atggcgaagg gggaatcgcc catcttctcc ttccggccat cactctgggc
      541 gtcggataca tggccctgct cgcccggacg acccgcgccg ccgtcctgga gatcctgagc
      601 gccgacttcg tgcggacggc ggcggccaaa ggcctctccc gcctcacggt caattcgaag
      661 cacgtgttcc ggaacgccct gatcctcgtc ctgacgacag ccggactcca gtttggctcg
      721 ctcatgggtc agaccgtcat cgtggagaag ctcttctcct ggcccggcat cggctccctg
      781 ctggtggaca cgatcttcca gcgggatatc cctgtggtgc aggggtgcgt gctgttgatc
      841 atcctgattt tcctggtggt caacctcgcc gtggatgtgc tgtacgcgct gatcgacccg
      901 cggattgagt accaatgagc gagacggtcc ggcagatccg acggtgcaag aacctgatga
      961 tcggggggat catcaccctg gcgatcgtat gcgtggcgct gctcgcgccc ttcctggcgc
     1021 cctatgatcc catcaaccat gcggacctcc tgcattcgga ggagccgccc agccgcacct
     1081 tcttcttcgg aacggacacc cagggcaggg acattctgtc ccgggtgatc ttcggcgcca
     1141 ggatttcgtt gagcatcggt ctgatcacgc agtccctgaa cagcctcatc ggaatcctgc
     1201 tgggcctcac cgcaggcttc ctggggaggt ggtgggacga tctggtcatg ggactgacca
     1261 acgtcatgct ctccatcccc gccctggtgc tggccctggc gatcatggcg gtcctggggc
     1321 cggggctgtt caacgtgttc ctggccatcg gcttcaccaa ctggtcctat agctgccggg
     1381 tcacccgatc ccaggtgctg gccacccgct ccctggacta cgtcaccgcc gcccgggccc
     1441 tcggctacgg ccgcgtgcgt atcatgctga cccaaatcct gcccaatatc gtggggccga
     1501 ttctggtgat cgccaccctc ggcgtggcgt atgcgatcct gaccgaggcc tccctgtcct
     1561 tcctcggtct gggggcgcaa ccacccgccc ccagttgggg tggcatgctc tccaccgctc
     1621 gggaacagct cttcacggct ccctggatct ccatcttccc cgggctggcc atcttcgtca
     1681 cggtgttggg cctgaatctg ctgggagacg ggttgcggga catcctggac ccgcacacct
     1741 tacggcaggg gcgatagacc gccaaccgcc ggacgccaag tgcgtcaagg agctcacatg
     1801 gacctagcaa tcctttcgag ccggatcttg acgggcgatc ccggccggcc ctgggccgag
     1861 gccctgtgca tcaaggatga cagggttgcc gccattggga ccaacgccga agctcggcat
     1921 gcctccggtc cggggacccg aatcttcgag ctccccgatc gactggtcac tccggggatc
     1981 gtggatgccc atctccactt tgtgaacttt ggcctgtatc tccagcgcgt ggacctgcgc
     2041 gatctcacct ccattgcggc ttgtcgcgag cgagtgaagg cggccgtcgg gaagaagaaa
     2101 cccggcgagt gggtcatcgg acgggcctgg aacgagcaca tctggagcga ccgccgtcag
     2161 ccgaatgcca aggatcttga cgacatttcg ccggatcacc cggtcatgct ggtccgctgc
     2221 tgcgggcata gcgtgtggct caacggcctg gccatgaagc tggcgggcat cacccaggaa
     2281 accgtggacc ccccggcggc gcggatcgag cgcgatgccg gtggaaaccc gatcggtctg
     2341 tgccacgaat accgaaagat catcgaaaga atcatcccgc cgccaagcct ggatgagagg
     2401 aaagcggcgg cgctccgggc ccaggccgag gcgctgcgcc acggcgtgac aggggtgcat
     2461 tcctgcgaga cgctgcagga atgggatgcc ctcgccgccc tggaggccga gggcagcttg
     2521 aaggtccggg ttcatcacac gctcccgccg gaggaagtgg cgacggctca ttcccgcggc
     2581 atcgaaccgg gcaaagggag cgagcgcttg tggttcggcc aggtgaagct cttcgccgac
     2641 gggtcactgg gctccggcac ggcgctgctg catgcgccat acgccgacga cccagccagc
     2701 acgggtctgg aggtcctctc ggttgccgag ctgcaagagc ggatcgagtt ggcctatcgg
     2761 tacgggggcg atgtggcggt gcacgccatc ggggacctgg cgacgaccaa cgtcctggac
     2821 gccatcgagg gggcgcgaaa gaagttccat ggtccgaagc gggaccggat cgagcatgtt
     2881 cagctcatgc acccgagtga cttcgctcga ttccgggtca tggaagtggt cgcctcctgc
     2941 cagcccgtgc acctccacac cgacatgccg gtggcggaga agaagtgggg aatggaccga
     3001 tgccggtacg gctatgcgtg gaagtccatg ctcaaggaag gaattcgcct ccagttcggc
     3061 tcggatgc
//
LOCUS       DBNGZC010000608         2279 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2304431, whole genome shotgun sequence.
ACCESSION   DBNGZC010000608 DBNGZC010000000
VERSION     DBNGZC010000608.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2304431"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="AC16G0_10555"
     CDS             <1..216
                     /locus_tag="AC16G0_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100088.1"
                     /translation="VMLGHPGCSGSTPWRCGVCRKELRGTWAVLTGRPFPVCSRCRLM
                     VHHGCLAQTDPPICRRCAEAQGQPSGG"
     gene            complement(266..1720)
                     /locus_tag="AC16G0_10560"
     CDS             complement(266..1720)
                     /locus_tag="AC16G0_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5100089.1"
                     /translation="MPYIEDLTISISTVRRESGGTHLAMRAAFLTAVYRREASMTPRI
                     LVVDDDSLIRRIMRDTLSTLPADVSEAKDGDEALKLAKAERPDLIFLDTMMPGMDGFQ
                     VAELLKQEPLTAGIPLVFVSALGTASHKVRGLDLGAEDYMAKPIDPEELKARVRSILR
                     RTRPSAPTPEPAASTVASGKLQAMPLPSLVRWLELERRSARLSLVRAGEAGEITFLDG
                     RISHALQGPRRGEAAVYQLLTWREGGFEILPATEGELPAGGELTRANEDLLQEGGRRM
                     EEASALRAGLPGSEALLEIPAALRTAAAAVVPPDGASLIALLDGKMDVGQILADSPFD
                     AWMTLKILHRLLRVGALSWVPAPGASGQAEAPRRSILRLLADAPVQYQSLRALQEAGR
                     FTLSARGMFVQTPTPFEVGEQVLLRFQLPGGTTWITAVGQVVWRNADAKKSKPEDLGM
                     MLQFIQASAEVLAAIEDCLTHGIVAQIRTAMEAP"
     gene            2173..>2279
                     /locus_tag="AC16G0_10565"
     CDS             2173..>2279
                     /locus_tag="AC16G0_10565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HIR5100090.1"
                     /translation="MKRKQTFELDRRDFMKAAGVVGGSAMLGLGPFRTL"
BASE COUNT          391 a          765 c          728 g          395 t
ORIGIN      
        1 gtgatgctgg gccaccctgg ctgcagcggg agtacaccct ggcgctgcgg cgtatgccgg
       61 aaagagctac gcggaacgtg ggcggtgctg acgggccgac cgtttccggt ctgctcccgc
      121 tgccggctga tggtccatca cggatgtctc gcccagaccg atccccccat ctgtcggcgt
      181 tgcgccgagg cgcaaggcca gccgtcaggt gggtagcccg cctccgaccc gaccattcga
      241 tccacccccg aaggggctcg ccacctcagg gtgcctccat cgccgtgcgg atctgagcca
      301 cgatgccatg cgtgagacag tcttcgatgg cggcgaggac ctctgctgaa gcctggatga
      361 actgcagcat catgccgaga tcctccggct tgctcttctt cgcgtctgcg ttgcgccaga
      421 ccacctgccc gacggccgtg atccaggtcg ttcctcctgg gagctgaaac cgaaggagca
      481 cctgctcgcc cacctcgaat ggagtcggtg tctggacgaa catcccacga gccgataggg
      541 tgaagcggcc ggcctcctga agcgcgcgca ggctctggta ctggaccggg gcgtcggcca
      601 gcaggcgaag gatgctgcgg cgaggcgcct ccgcctgtcc ggaagcgcca ggtgcaggca
      661 cccaactcag ggccccgacg cggagcaggc gatggaggat cttcagggtc atccaggcgt
      721 cgaagggact gtccgccagg atctggccca catccatctt gccgtccagc aaggcgatca
      781 ggctggcgcc gtcaggagga accactgccg ctgcggcggt acgaagggcc gccgggatct
      841 ccagcagtgc ttccgatccg ggcagcccgg cccgcaaggc agaggcttcc tccatccgcc
      901 ggccgccctc ctgcaggaga tcctcgttgg cccgtgtcag ctcgccaccg gctggcagct
      961 ccccctcagt tgccggcagg atctcgaacc ccccctcccg ccaggtgagg agctggtaga
     1021 cagcggcctc gccccgacgc ggcccctgca gcgcgtggct gatacgccca tccaagaagg
     1081 tgatctcgcc cgcctcaccg gcccgcacca gagatagccg agcgctccga cgctccaatt
     1141 ccagccaacg caccaaggag ggcaggggca tggcctggag ctttccgctg gccacggtcg
     1201 acgctgccgg ctcgggagtc ggggccgagg gacgagtccg tcggaggatg gagcggaccc
     1261 tggccttgag ctcttcgggg tcgatcggct tcgccatgta gtcttccgcg cccaggtcaa
     1321 gaccccgcac cttgtgactg gcggtcccga gggcgctgac gaagaccagg gggatcccgg
     1381 ccgtcagcgg ctcctgcttc agcagctctg cgacttgaaa cccatccatt cccggcatca
     1441 tggtatccag gaagatgaga tccgggcgct cggccttggc cagcttcaga gcctcatcac
     1501 catcctttgc ctcggagacg tcggcaggca gggtcgagag ggtgtcccgc atgatgcgac
     1561 ggatcaggga gtcgtcgtct accaccagta tgcgcggcgt catactcgcc tccctccgat
     1621 acacggcggt caagaacgcg gcccgcatcg ccaggtgcgt gccgcctgat tcccggcgga
     1681 cggtggagat cgagatggtc aggtcttcta tatagggcat cagaccacat ccagggcagt
     1741 cgagtcaaga gcgacctggg gaactcgccc caggaactct tccatgagtc agcgagacat
     1801 cctcaggcac ggacaccagc tccatcggcc ccttggatca agtccgcaat tcctgttgac
     1861 acgccggagc gctgagcgat aggctccctg caagcctccc ggcatcattc ccgtcaggct
     1921 ggagcgctca cccacagcat tccccgctgt gcgcggcggg ccgctccggt ccctaatttt
     1981 ggcttgtatt cagttggtgt ccggcgccaa ttcttctgga cgaccgataa acccaggggg
     2041 cgcttccctg tcgggagaca gggcctggtg ttcccagatg ttcccctggg caccagtttc
     2101 tgatctggca tgacatattc caagtcgagc tggaactaaa cccttgttgc gtcggatgaa
     2161 gaggaggggg ccatgaaacg gaaacagacc ttcgaattgg atcgccgcga tttcatgaag
     2221 gcggcaggcg tcgtgggggg gtcggccatg ctgggcctcg gtcctttccg gaccttgat
//
LOCUS       DBNGZC010000609         7927 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_53937, whole genome shotgun sequence.
ACCESSION   DBNGZC010000609 DBNGZC010000000
VERSION     DBNGZC010000609.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7927)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7927)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7927
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_53937"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1360)
                     /locus_tag="AC16G0_10570"
     CDS             complement(20..1360)
                     /locus_tag="AC16G0_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011093113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HIR5100091.1"
                     /translation="MDFFIQLAIVLVCLFYGARKGGIALGLLGGIGMVIMVYVFRLTP
                     GRPPVDVMLTIIAVVAASATLQSSGGLDVLLQLAERMLRRNPRFISILAPFTTCGLTM
                     LCGTGHVVYTMLPIVYDVAIKNNVRPERPMAASSIGAQMGIIGSPVSVAVVSLVAFLA
                     KAPIAGRAIDFIQLLSITIPSTLAGVLAIGIFSWFRGKDLDKDEEFQAFIAKPENREY
                     VYGSSASLLGKTLSRNQWTAMFLFSGAIAVVALLGAFEGLRPQIGGTPMNMVLTIQMF
                     MLLAGALIVIITKTDAKAIGKSEVFRAGMVAVVAVFGIAWMADTMFEAHVPALKSMLT
                     GVVKAYPWTYALVLLIVSKFVNSQAAAIAAIVPVALSIGVHPGYVVAFSAACYGYYIL
                     PTYPSDLAAIQFDRSGTTRIGKYVINHSFILPGLIGVSTSCVVGYFIALSRGLV"
     gene            complement(1751..1903)
                     /locus_tag="AC16G0_10575"
     CDS             complement(1751..1903)
                     /locus_tag="AC16G0_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100092.1"
                     /translation="MVQDPEFKSAMTKLETPIAYLDAPDFQKFWDKDAKMLADAIKRV
                     GKIEEK"
     gene            2105..2584
                     /locus_tag="AC16G0_10580"
     CDS             2105..2584
                     /locus_tag="AC16G0_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5100093.1"
                     /translation="MPDDKALREDVLLLLRGGRAHVSFEKAVANLPEELRGKKPRAVP
                     YSPWQLLEHLRICQWDILEYIRNPQHVSPEWPDGYWPKEAAPARNAWAESVKAFQADR
                     QALVDMAADTSKDLLARIPHETDGPTILHELLLVADHNAYHLGQLVILRRLLGAWEG"
     gene            complement(2774..3301)
                     /locus_tag="AC16G0_10585"
     CDS             complement(2774..3301)
                     /locus_tag="AC16G0_10585"
                     /inference="COORDINATES: protein motif:HMM:NF046506.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6941 family protein"
                     /protein_id="HIR5100094.1"
                     /translation="METPQQGNPASVTAPHPACNAMLICDQAVQEGATGKTSLVGIFE
                     NISAFQYPARCGFVTVYAKLTDAQGEYRIRLELINLGDFTVIGQREFRATIGDRMVPA
                     ELVFQIGGLVFEKPGRYEVRLYANERWVGSKSLYLVQAAEPIPPEQIRVSGSTVTVRW
                     LSFMLPSAKPLADAP"
     gene            3708..4775
                     /locus_tag="AC16G0_10590"
     CDS             3708..4775
                     /locus_tag="AC16G0_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HIR5100095.1"
                     /translation="MAVEGHASSFPAKRLEAFITSVLTALGLPEQDAATCAARMTDAD
                     LRGVDTHGIFRLPHYCQRIRAGGINLRPKVRPVRENVVTALVDWDNGMGHVVVTYAAE
                     LAIQKAAETGLAWVGTFNGNHAGAAAAYTTLALAHDMICMYMTVANGNHMPPWGGVEP
                     ILGTNPISVAIPAGEEPPIVLDMATTVTSHGKVKLAAQKGESMPVGWMVDRKGQPLTD
                     PKRAAEGFLLPIGMYKGYGLNVVIGMLAGVLNGAAFGRNVVDFNKDFATRNNSGHMIL
                     AMPVNNFQTADTFKKEMDSVIREIRESERMEGVDRIWLPGEIEYYRTRERLDKGIPIA
                     PAVLDGLRKLAGELNLLDRLE"
     gene            4833..6884
                     /gene="glgX"
                     /locus_tag="AC16G0_10595"
     CDS             4833..6884
                     /gene="glgX"
                     /locus_tag="AC16G0_10595"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120549 - limit dextrin
                     alpha-1,6-maltotetraose-hydrolase activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HIR5100096.1"
                     /translation="MADSEVRPGKSNPLGATPFRDGVNFSVFSKSARAMELLLFDAPD
                     DPRPAHVIPLDPQKNRTYHYWHVLVGGLRPGQHYGYRAIGPFTPEQGLRFDRDKILLD
                     PYGRGVAVPRGYDRQAASRPGDTSATAMKSVVADLRAYNWEGDAPLRRAFAKTVIYEM
                     HVGGFTRHPGAGVDPPRRGTYAGLVEKIPYLKELGITAVELLPVFQFDPQDCPQGLVN
                     YWGYCPVSFFAPHHGYSSRPDPLGALNEFRDMVKALHRAGIEVILDVVYNHTAEGDQN
                     GPTICFRGLENEVYYLLEADRARYADYTGTGNTLNTNQSIVRRLILDSLRYWVGVMHV
                     DGFRFDLASILSRDESGRPLKVPPVLWDIESDPVLAGTKLIAEAWDAGGLYQVGSFVG
                     DSWKEWNGLFRDDVRSFVRGDCGTVSRLASRLLASPDLYGHKEQEPEQSINFVTCHDG
                     FTLNDLVSYDAKHNEANGEGNQDGSDANLSWNCGVEGPSDDPAVERLRSRQVRNFLTI
                     TLLSLGTPMLLMGDEVRRTQRGNNNAYCQDNELGWFDWSLVSRHAGLHRFVQQLIRFR
                     LSWDTTTQRTGLSLNQLLRQAQFEWHGVRLHQPDWSHESHSLAIMARVMEGRLDLHLM
                     LNAYREPLTFELPLSRWRRVLDTALESPEDILPWASAPTVSGSTCVVQPRSVVLLLAQ
                     P"
     gene            7393..>7927
                     /locus_tag="AC16G0_10600"
     CDS             7393..>7927
                     /locus_tag="AC16G0_10600"
                     /inference="COORDINATES: protein motif:HMM:NF012675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100097.1"
                     /translation="MDVLNDPAGREQLLRLGARSVPVIAKGDKFVFGQMLEDVAEFVG
                     LAGTGQQKLSAAQLVSKYRVILDAAKRLIAQVPDQRLHERVIPNRDRTIRVLCHHMFR
                     IPESFIQACDGTEYTEQFHSLPPAADIQTVRQIVTYGESVRAEFDAWWDRVEDKSCRK
                     MLVTYFGPCPAHDLLERS"
BASE COUNT         1494 a         2465 c         2557 g         1411 t
ORIGIN      
        1 tcatcgaagg gcttgaagac tagaccagcc ccctgctcag cgcgatgaag tagcccacca
       61 cgcacgatgt cgacacgccg atcaaaccag gcaggatgaa gctgtggttg atcacgtact
      121 ttccgatgcg ggtggtgccg gaccggtcga actggatggc ggccaggtcg ctggggtagg
      181 tggggaggat gtaatagccg tagcacgcgg ccgagaacgc gaccacgtag ccgggatgca
      241 ccccgatcga cagcgccacg gggacgatgg ccgcgatggc ggccgcctgc gaattcacga
      301 atttcgacac gatcagcagc acgagcgcgt aggtccacgg gtacgccttc acgacgcccg
      361 tgagcatgct cttgagggcc ggcacatgcg cctcgaacat cgtatcggcc atccacgcga
      421 tcccgaacac cgcgacgacc gccaccatgc cggcgcggaa cacctcgctc ttgccgatcg
      481 ccttcgcgtc cgtcttcgtg atgatgacga tcagcgcacc cgccagcagc atgaacatct
      541 ggatggtcag caccatgttc atgggcgtgc cgccgatctg cgggcgaagg ccctcgaatg
      601 cccccagcag ggccaccacc gcgatggcac cgctgaacag gaacatcgcg gtccactggt
      661 tcctcgacag cgtcttgccg agaagcgacg cgctgctccc gtacacgtat tcgcggttct
      721 cgggtttcgc gataaacgcc tggaactctt catccttgtc caggtccttg ccgcggaacc
      781 agctgaaaat accgatggcg aggacgccgg ccagcgtgga cggaatggtg atggacaaga
      841 gctggatgaa gtcgatggcc cggccggcga tcggcgcctt ggcgaggaag gcgaccagcg
      901 agacgacggc gaccgagacg gggctgccga taattcccat ctgggcgccg atcgagctgg
      961 cggccatcgg gcgctctggc cggacgttgt tcttgatggc gacgtcgtag acgatcggca
     1021 gcatcgtgta gacgacgtgg ccggtcccgc agagcatcgt caggccgcag gtcgtgaagg
     1081 gcgcgaggat ggaaatgaac cggggattcc gccgcagcat ccgctccgcg agctgcaaca
     1141 gcacatcgag cccgccggac gactgcagcg tggcggaggc ggcgacgacg gcgatgatgg
     1201 tcagcatcac atccaccggc ggccggccgg gggtgagccg gaagacatag accatgatga
     1261 ccatgccgat gccgccgagc aggcccaggg ctatgccgcc tttgcgggcg ccgtagaaga
     1321 ggcagacaag gacaatcgca agttgaatga agaaatccat agctcgttct cccagtcggc
     1381 tgaggagttg tccatctgcg catcggatag gaagttatcg gatggacaac tcggggaaat
     1441 cagaactggc ggggacgcta gcaccggcgg ccagaggagt caaggcatga gccgacggtt
     1501 gtatctgttg taaacagaca tgggtgacac ttccaggctc gtagtatcga ccaggattgc
     1561 atcacccggg aaggaggtca ctcatgtcgt aatgccgggg acatcataca gaactccgtg
     1621 ctgctagacc tccgcctgtc gctgagcacc tcttctacgc tgagtaactg aggaacacct
     1681 cgccctccta ctattgggac caactgccag ggacaagacc tgtcaagcac aggggccctg
     1741 aagcgaaagc ctacttctcc tcgatctttc ccacccgctt gatggcgtcg gccagcatct
     1801 tggcgtcctt gtcccagaac ttctggaagt ccggcgcgtc caggtaggcg atgggggtct
     1861 ccagcttggt catggcgctc ttgaactccg ggtcctgaac cacggcctgg gccgtgtcag
     1921 ggtgtccttc cgaaaatggc tttcacgccg acgctcagtg ctctacagta ttgaacggct
     1981 atacgctgag ccattcgccc gagccaacgg gctgagagcc tatccaagtg atcgaagctt
     2041 gaggagcctg ggcgggcttg gagccttggg gcggaattga atccgaggaa aggagaggtc
     2101 aaagatgccg gacgacaaag cgctgcgtga ggacgtgctg ctcctgctgc gtggcggccg
     2161 ggcacatgtc agcttcgaga aggccgttgc caaccttcca gaagagctca gaggcaagaa
     2221 gccgcgtgcc gtgccgtact ccccatggca actactggag cacttgcgga tctgccagtg
     2281 ggacatcctc gaatacatcc gcaatccgca gcacgtatcc ccggagtggc cggatgggta
     2341 ctggccgaag gaggcggcgc cggcacggaa tgcgtgggcc gagagcgtga aggccttcca
     2401 ggcggaccgg caggccctcg tcgacatggc ggccgatacg tccaaggacc tgctggcgcg
     2461 gatcccgcac gagacagacg ggcccacgat tttgcacgag ctgctcctgg tggccgacca
     2521 caacgcctac cacctgggac aactggtcat cctgcggagg ctcttgggcg cgtgggaggg
     2581 ctgacccaga ttattcacga aagtttcgtg aaaaatctgg attcaatgac caatgacaaa
     2641 tttccaatga ccaatggtgg tgggcgcgta caccgcgccg tcccggaaca tcccggaggg
     2701 ggaatccgca attggtcctt gggatttggt cattcccccg attattcgcg aactcgttcg
     2761 cgaataatcg ggatcagggc gcgtccgcga ggggcttggc ggagggaagc atgaaactca
     2821 gccagcgcac ggtgacggtg gagccggaca cgcggatctg ctccggaggg atcggctcgg
     2881 cggcttgcac gaggtacaga gatttgctgc cgacccagcg ctcgttggcg tacaggcgga
     2941 cttcgtacct gccgggcttc tcgaacacca agccgccaat ctgaaagacc agctctgccg
     3001 gcaccattcg atcgccgatg gtcgcccgaa actccctctg cccgatcacc gtgaaatcgc
     3061 cgaggttgat gagctccaac cggatgcggt attcgccctg ggcgtccgtg agcttggcgt
     3121 acacggtgac gaaaccgcag cgagcggggt attggaaggc actgatattc tcgaagatcc
     3181 ccaccaggct agtttttccc gtggccccct cctggacggc ctggtcgcag atgagcatgg
     3241 cgttgcacgc cgggtggggc gcggtaacgc tggccgggtt accctgctgt ggggtctcca
     3301 tgccgacgat cctagcacag actacggctt cgccacaaca ggggctcctt tattcggcga
     3361 agagtcctgt gtcctgtccc agaggctcgc taaacaagtc cggccgttcc ggcccgcccc
     3421 tccccagcct gatcggggtc aacacccttc aactggtctc cggtacctgt tgggggtctt
     3481 gcttgacatc gccttcccgc tcttggtagc gtccccatcg cgtggaatgg ttgccctgag
     3541 tagcgccgtg tcttggcctc acggcggctt cctcggtgcc gtggtgccat tgcccttttc
     3601 gggccgagag ccgaacccac cttttgtctt taggcaattg attggatggc tgtgccgttc
     3661 tccttacgct taccgccgac cgcactaggt cagattggag gaatcctatg gcagtcgagg
     3721 gacatgcatc cagctttcca gcaaagcgat tggaggcttt catcacgagt gtcctgacgg
     3781 ccctggggct tcccgaacag gacgcggcga cctgcgccgc ccgcatgacg gacgccgatc
     3841 tccggggcgt ggatacgcat ggcatcttcc gcctcccgca ttattgccag agaatccgag
     3901 cggggggaat caatcttcga ccgaaggtcc gccccgtgcg cgagaacgtg gtgaccgcgc
     3961 tggtggactg ggataacggc atgggtcatg tagtggtgac ctatgccgcc gagctggcga
     4021 ttcaaaaagc tgcagagacc ggtctggcgt gggtgggcac cttcaacggg aatcacgccg
     4081 gtgccgccgc cgcatacacg acgctggcgt tggcccacga catgatctgt atgtacatga
     4141 cggttgccaa cggcaatcac atgccgccct ggggtggcgt cgagccgata ctcggcacga
     4201 acccgatttc ggtggccatc ccggccggag aagagcctcc catcgttctg gatatggcaa
     4261 cgacggtgac ctcccacggg aaggtgaagc tggcggccca gaagggggag tccatgcccg
     4321 tcggatggat ggtggatcgc aaggggcagc ccctcaccga ccccaagcgc gctgccgagg
     4381 ggtttctgct cccgatcggg atgtacaagg gctacggatt gaacgtggtc atcggcatgc
     4441 ttgccggcgt gctcaatggc gcggccttcg gcagaaacgt cgtggacttc aacaaggact
     4501 ttgcgacgag gaacaacagc gggcacatga tcctggcgat gccggtgaac aacttccaga
     4561 ccgccgacac cttcaagaaa gagatggact ccgtgatcag agagatccgg gagtcagagc
     4621 gcatggaggg cgtggatcgc atctggctgc cgggggagat cgagtactac cggactcggg
     4681 agcggctgga caaggggatc ccgatcgccc cggccgttct cgacgggctc cggaagctgg
     4741 ccggcgagtt gaatctgttg gatcggttgg agtagcccga ggtttccatc tgccattcgg
     4801 ttcgtcggca tggcctcgaa ggagaactgc ggatggcgga ttcggaggtc cggccgggca
     4861 agagtaaccc gctcggggcg accccgttcc gggacggcgt gaacttcagc gtgttctcga
     4921 agagcgcccg cgctatggag ctgctcctct tcgacgcacc ggacgatccc cggcccgccc
     4981 atgtgattcc cctggacccc caaaagaacc gcacgtacca ctactggcac gtgctcgtgg
     5041 gcggcctccg gccgggccag cattacgggt atcgcgccat cggccccttc acgcctgagc
     5101 aggggctccg gttcgaccgt gacaagatcc tgctcgatcc ctacgggcgc ggcgtcgcgg
     5161 tgccgagggg atacgaccgc caggcggcga gccggccggg cgacacctcg gccaccgcga
     5221 tgaagagcgt cgtggcggat ctccgcgcct ataactggga gggggatgcg ccgctacggc
     5281 gagccttcgc caagacagtg atctacgaga tgcacgtggg cggcttcacg cgacatcccg
     5341 gtgccggggt ggaccctccg cggcgcggga cctacgccgg cctggtggag aagatcccct
     5401 atctcaagga gctggggatc accgctgtcg agctgctgcc ggtctttcaa ttcgaccccc
     5461 aggactgccc gcagggcctg gtcaactact ggggctactg tcccgtctcg ttcttcgccc
     5521 ctcaccacgg ctacagctcc cggccggacc ccctcggcgc cctgaacgaa ttccgggata
     5581 tggtcaaggc gctccatcgc gcgggcatcg aggtcatcct cgacgtggtg tacaaccaca
     5641 cggccgaggg cgaccagaat gggccgacga tctgcttccg cggtctggag aacgaggtct
     5701 actatctcct ggaggccgac cgggctcgct acgcggatta caccggcacc ggcaacaccc
     5761 tgaacaccaa ccagtcgatc gtgcgccggc tgatcctcga cagcctccgc tattgggtcg
     5821 gggtgatgca cgtggacggc ttccggttcg atctggcgtc gatcctgtcc cgggacgagt
     5881 ccggccggcc cctcaaggta cccccggtgc tgtgggacat cgagtcggat cccgtgctgg
     5941 ccggcaccaa gctgatcgcc gaggcctggg acgccggcgg cctctatcag gtcggtagct
     6001 ttgtcggcga tagctggaag gaatggaatg gattgttccg cgacgatgtt cggagcttcg
     6061 tcagggggga ttgcgggact gtgtcccggc tcgcatcccg gctgctggcg agcccggatc
     6121 tgtacggtca caaggagcag gagcccgaac agagcatcaa cttcgtgacc tgccacgacg
     6181 gcttcacgct gaacgatctc gtttcctacg atgccaagca caacgaggcg aatggcgagg
     6241 gcaaccagga cggctccgac gccaacctga gctggaactg cggcgtggag gggccgagcg
     6301 acgatccggc ggtggagcgg ctgcgcagcc ggcaggtgag gaacttcctg accatcaccc
     6361 tgctctctct gggcacgccc atgctgctca tgggggacga ggtccggcga acccagcgcg
     6421 gcaacaacaa cgcctactgc caggacaacg agctcggctg gttcgactgg tccctggttt
     6481 cgcggcacgc cggcctccac cgattcgtgc agcagctcat ccgcttccgg ctgagctggg
     6541 acaccacaac ccagaggacc ggcctcagcc tcaaccaact cctgcgccag gcgcagttcg
     6601 agtggcatgg cgtccgcctc caccagccgg attggagcca cgagtctcac tcgctggcga
     6661 tcatggcccg ggtgatggag gggcggctcg acctgcacct tatgctgaat gcctaccgcg
     6721 agcctctgac cttcgagctg ccgcttagcc gctggcgtcg ggtgcttgac acggctctgg
     6781 aatcgccgga ggatatcctc ccctgggcgt ccgcaccgac ggtctcggga tccacctgtg
     6841 tggtccagcc ccggtcggtc gtgctgctgc tggcccagcc ctgacccagg gcgccgcccg
     6901 gaaagttgtt tcccctcaag ggcttcctcc tttgatggta taatgtctcc caattgaatc
     6961 gttgctgagg agtcagccgt gcgggacctg agggttatac gccaagggtg ggaggaggtc
     7021 gagcgggcag agaaactcct gctttgtcgg atgaccgctc aggagagttt ccgccagtgg
     7081 ctgcagttgc agcgcctgtt cgagccccag ctacaagaga cctgggcgct ctttgggccg
     7141 gagcgtcact cggctctggc cgagcttcaa actcgcctac gccgcttggc tgagtggcag
     7201 gagaagcatg gagagcctgc ttcggtccgt tcggaagctc caacagcgcc tgctggaagc
     7261 tgacattccc tccgtcagga ggcgatgatg gcaggagcgg cggacaggtt cgtgctgtac
     7321 acccagccgg ggtgaagcag ctgcctcaga gcgaaagagt ttctttcgaa acaaggtgtg
     7381 gacttcatcc tggtggacgt gctcaacgat cccgccggac gcgagcaatt gctgcggctc
     7441 ggcgcgcgca gcgtcccggt gatcgcgaaa ggcgacaagt tcgtcttcgg gcaaatgctg
     7501 gaagacgtcg ccgagttcgt gggcctcgcc ggcaccggcc agcagaaatt atccgcagcg
     7561 cagctcgtgt ccaagtaccg ggtcatactc gatgcggcca aacggctgat cgctcaggtg
     7621 ccggaccagc gcctccacga gcgtgtcatt ccaaaccgcg accgcacgat ccgggtgctg
     7681 tgccatcaca tgttccgcat ccccgagtcg ttcatccagg cgtgcgacgg caccgagtac
     7741 acggagcaat tccacagcct cccgcctgca gccgacatcc agacagttcg gcagatcgtc
     7801 acctacggtg agtcggtgcg cgccgagttc gatgcgtggt gggaccgggt ggaggacaag
     7861 tcctgccgga agatgctcgt cacgtacttc ggaccatgcc cggcgcacga cctgctcgag
     7921 cgctcga
//
LOCUS       DBNGZC010000610         2076 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1914241, whole genome shotgun sequence.
ACCESSION   DBNGZC010000610 DBNGZC010000000
VERSION     DBNGZC010000610.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1914241"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="AC16G0_10605"
     CDS             complement(<1..974)
                     /locus_tag="AC16G0_10605"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR5100098.1"
                     /translation="MRYRYLGSSGLLISRITLGTMTFGAPDWGCDEREAHAILKRFLD
                     GGGNSIDAADVYAGGRAEEIIGTFLPQTPREQVVLASKCYFPMGTQPNHHGLSRKHVI
                     AACEASLRRLRTDYIDVYYLHGPDPITRLEETLRAFDDLVRQGKVRYVGVSNLFAWQL
                     AKAAGVAARMNLEPPVAAQYIYSLIHREPEREIIPAAVDHGLGILCYGALGAGLLTGK
                     YRGMTEPPKGSRMQRRMQIDGARFWHPTGLKVAEILEEASAKTGIPMGKLAIAWPLGR
                     KFVASVIVGVRSCEQLASNLEPGDWDMPEDVWMSLEEQTRPEEEYTTW"
     gene            1221..>2076
                     /locus_tag="AC16G0_10610"
     CDS             1221..>2076
                     /locus_tag="AC16G0_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR5100099.1"
                     /translation="MSGSHGRRDVLKLAGFGVAAGVAAGVGGLSAGLLSPEQAYAQAQ
                     PGSLLRTVLDRKKLIVGTGSTNAPWHFEDEKGQLTGMDIAMGRILAAGLFDDANKVEF
                     VKQDPSARIPNITTGKVDIVIQFMTISPARAQLINFTRPYYVEGAALLTSPKSKHKTY
                     KQLMAAGKTVKASVLQNVDADKLVHDGLPQAQVMQLDTQANVIQALDSGRVDVAVVDL
                     STVRWLVKRNPTGYVDAGYSYYPMLYGAGVRQGDLDWLHWVDTCLNVAMFGHQTEVYD
                     KAFEEFFGQ"
BASE COUNT          380 a          683 c          658 g          355 t
ORIGIN      
        1 aaccaagtgg tgtactcctc ctccggtcgg gtctgttcct ccagactcat ccacacatcc
       61 tcgggcatgt cccaatcgcc cggctccagg ttggacgcaa gctgctcgca agagcgcacg
      121 ccgacgatga ccgaggctac gaacttccgg cccaggggcc aggcgatggc gagcttcccc
      181 atggggatcc ccgtcttggc ggaagcttcc tcgaggattt ccgccacctt gagccctgtt
      241 ggatgccaga aacgggcccc atcgatctgc atccgccttt gcatgcggct gcccttcggc
      301 ggctcggtca tacccctgta tttcccggtg agaagacccg ccccgagtgc accgtagcag
      361 aggatgccga gaccgtggtc caccgccgcc gggatgatct cccgctcagg ctcccggtgg
      421 atcaaactgt agatgtactg cgcagcgaca ggaggctcca gattcatccg ggcggcaacc
      481 ccggccgcct tggcgagctg ccaggcgaag aggttgctca cgccgacata ccgcaccttc
      541 ccctgtcgga cgaggtcgtc gaaggcccgc agggtttcct ccagccgggt gatggggtcg
      601 ggaccgtgaa ggtagtagac gtcgatgtaa tcggtccgca atcggcgcag gctggcctca
      661 caggcggcga tgacgtgttt tcgagagaga ccgtggtggt tgggctgggt gcccatggga
      721 aagtagcact tggaggccag gacgacctgc tcccgcgggg tctgcggcag gaaggtcccg
      781 atgatctcct cggccctgcc gccggcgtag acatccgccg catcgatgga gttgcccccg
      841 ccgtccagga atcgcttcag gatggcgtgg gcctcccgct cgtcgcaccc ccagtccgga
      901 gccccaaagg tcatggtccc gagcgtgatg cgcgagatga gcaggccgga cgacccgagg
      961 tatcggtacc tcatcgtttc ctcctgagag caaaggggat gcatcgaaac caccgaaaga
     1021 tagcccggcg cgggaagcgc cgtcaagcgc cgattaccgc ccgcggagcc tgggattcgc
     1081 gcccccgcgt ccgacgactt ttcccttgac actgctccaa tgctgctgga taaaacgact
     1141 ctattcctcg gcggcgggaa gcgcctcctc caggaggccc gccggtcctc ccgtgcaggc
     1201 agatcacaag gaggtgggtc atgagcgggt cccacggtcg tcgtgatgtt ctgaaactgg
     1261 ccggcttcgg cgtcgccgca ggtgtggcgg cgggggtcgg cggactctcg gccgggctgc
     1321 tcagtcccga gcaggcctac gcccaagccc agcccggcag cctactacgc accgtcctgg
     1381 atcggaaaaa gctgatcgtc ggcaccggga gcacgaacgc gccttggcat ttcgaggacg
     1441 agaaggggca gctcaccggc atggacatcg ccatgggccg catcctggcc gcggggctgt
     1501 tcgatgacgc caataaggtc gagttcgtca agcaggaccc ctcagcgcgc atcccgaaca
     1561 tcaccacggg caaggtggat atcgttatcc agttcatgac gatctcgccc gcccgggccc
     1621 agctcatcaa cttcacgcgg ccgtactacg tcgagggggc cgccctcctc acctccccca
     1681 agagcaagca caagacctac aagcagctca tggccgccgg gaagacggtg aaggcttctg
     1741 tcctgcagaa cgtggatgcc gacaagctcg ttcacgatgg gctgccgcag gcccaagtga
     1801 tgcagctcga cacccaggca aacgtcatcc aggcgctgga ctccgggcgc gtggatgtgg
     1861 ccgtggtgga cctctcgacg gtccgctggc tagtgaagcg caatccgacc ggatatgtcg
     1921 acgcaggcta cagttactat ccgatgcttt acggcgcggg cgtgcgccag ggcgacctcg
     1981 actggctgca ctgggtcgac acgtgcctca acgtggcgat gttcggccac cagaccgagg
     2041 tctatgacaa agccttcgag gaattcttcg gccaga
//
LOCUS       DBNGZC010000611         2839 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1423067, whole genome shotgun sequence.
ACCESSION   DBNGZC010000611 DBNGZC010000000
VERSION     DBNGZC010000611.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1423067"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="AC16G0_10615"
     CDS             complement(<1..897)
                     /locus_tag="AC16G0_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012876.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HIR5100100.1"
                     /translation="MRIATRLTLLLIAAMAVVMAAFGYLRARQERQHLITEQEQTTLL
                     LANAITLTVQHALRDRRPQDIRDLLVAMLRKPNPVDRIRIFDGRLEDIYSAVSDVAAS
                     TLVPPAELEQVLRGGQPIVRYLDAPRRPTVYVILPLKTQRGAIIGVLEVVHVASRVRR
                     QIRDAIRDTAIQLTLLSFTIGLVIWLTVRISIRRPLGHLMRTALAFGHGDLDRRIGIR
                     RRDEIGQLASAFNRMAERLQSAQVRLVAEGQERLKLEWQVQHAEKLAAVGRLASEVAH
                     EIGTPLSIISGRAEVVQRTLDAA"
     gene            complement(943..>2839)
                     /locus_tag="AC16G0_10620"
     CDS             complement(943..>2839)
                     /locus_tag="AC16G0_10620"
                     /inference="COORDINATES: protein motif:HMM:NF024516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HIR5100101.1"
                     /translation="LNPARTADLIALLPEDLEIQGAFQASGRLTGAPKALSGDVTLQL
                     ENVRTWDESWQQGNALLHFREGAVEITQISLRRGEEQLTGEIQLTADGILSGGLTSTP
                     TDLSRVGSLAGSDLAGRASFQLKFQGSLHETRTLGQVTATALFYREVPLGPGTASFRL
                     ERKALDVDLTLRDGAYQLHAAIGPPPNRSQQAVLSLRDADLDLVLQTAGVSAPSPWQL
                     RASGRIQVRGPAGDLAASQGEANFERMQLQGDGLAWESRDPVQLAWNQRALTLGALHL
                     RSGDYAVDIRGTAGGENQTDLTVIGTLPLAALPGWPPVLRPAGGLAIANLRLLGSWDA
                     PQFQGQLEIRQGRLILSGLSSQFTQVHATLDLAPHRTEIRDWRAQLAEGNIRGGAELV
                     RQDGGWKFRTTFEEDGGRAEQLLSGLYGGKGEVTGALSLGGLLASQGEVSADFWRNLN
                     GDLKLRMKNGRIGSYTVLAKILSLLNVRQALELKSAELDAEGMPYQNLTADIKIQHGI
                     ARTDNLVLDSRAMKVNAVGSVNLAEDTVDLKVAIKPFQTVDTIISHIPLAGWLLTGKE
                     KSLVVAYYQVSGSLRDPQVTAIPVQSVGRNVFGIFKNLLELPEVITGPYENLPPQAPK
                     PDEGQDR"
BASE COUNT          423 a          954 c          975 g          487 t
ORIGIN      
        1 agcggcgtcc agcgtcctct gcacgacctc cgcccgcccg gagataatgc tgagcggcgt
       61 gccgatctcg tgcgccacct cgctggccag cctgcccacg gccgccagct tctctgcgtg
      121 ctgcacctgc cattcgagct tgaggcgctc ctgcccctcg gccaccagtc gaacctgagc
      181 ggactggagg cgctcggcca tgcggttgaa ggccgaagca agctggccga tctcgtcccg
      241 acgcctgata ccgatccggc gatccagatc cccgtgcccg aaggccagcg ccgtccgcat
      301 caggtgcccg agaggccgcc gaatgctgat ccggacggtc agccagatga ccaggccgat
      361 ggtgaagctc aagagggtca gctggatcgc ggtgtcccgg atggcatccc ggatctgccg
      421 ccgcacccgc gaggctacgt ggaccacctc cagcacgccg atgatcgcgc cccgctgggt
      481 cttgaggggc aggatgacgt agacggtggg gcggcgcggg gcgtccaggt agcgcacgat
      541 gggttgcccg cccctgagga cctgttccag ctccgccggg gggaccagcg tcgacgcggc
      601 cacgtccgat acggcgctgt agatgtcctc cagtcggcca tcgaagatcc gaatgcgatc
      661 caccggattg ggcttccgca gcatggcgac cagcagatcc cggatgtcct ggggacgccg
      721 gtcgcggagt gcgtgctgca ccgtgagcgt gatggcgttg gccaagagca gggtcgtttg
      781 ctcctgctcg gtgatcaggt gctgccgctc ctgtcgggcg cggaggtagc cgaaggccgc
      841 catgacgacc gccatggcgg cgatcaggag cagcgtgagc ctggtggcga tacgcatgca
      901 ggggtcccca ccgccgtccg cgtcggcgat cgctggttcc ggctagcggt cctgcccctc
      961 gtcgggcttg ggtgcctgcg ggggcaggtt ctcgtaaggg ccggtgatca cctcgggaag
     1021 ctcaaggaga ttcttgaaga tcccgaagac gttccgcccg acggattgca cggggatggc
     1081 tgtcacctgc ggatcccgca aggagccgct cacctgatag taggccacca cgagactctt
     1141 ctccttcccc gtcaggagcc agccggccag ggggatgtgg ctgatgatcg tgtccaccgt
     1201 ctggaacggc ttgatcgcca ccttcaggtc caccgtatcc tcggccaggt tgaccgagcc
     1261 cacggcgttg accttcatgg ctcggctgtc caagaccagg ttgtcggtcc tggcgattcc
     1321 gtgctggatc ttgatgtcgg ccgtgaggtt ctgatagggc atcccctcgg cgtccagctc
     1381 ggccgacttc agttccaggg cctgccggac gttcaggagg gacaggatct tggccaggac
     1441 ggtgtagctc ccgatgcgtc cgttcttcat cctcagcttg aggtccccat tcaggttgcg
     1501 ccagaagtcg gccgagacct cgccctggct tgccaggagc ccgcccaggg ataaggcccc
     1561 ggtgacttcc cccttgcctc cgtagagtcc gctgaggagc tgctcggccc gcccgccatc
     1621 ctcctcgaaa gtggtccgga acttccaccc gccgtcctga cgcacgagct cggcgccccc
     1681 tcgaatgttc ccctcggcga gctgtgcacg ccaatcacgg atctccgtgc ggtgcggcgc
     1741 aaggtccagg gtggcgtgga cctgggtgaa ttgcgatgag agccccgaca ggatcaggcg
     1801 gccttgccgg atttccagct gtccctggaa ttggggtgcg tcccaggatc cgaggaggcg
     1861 cagattggcg atggccagtc cacccgcggg tcgcagcacg ggaggccatc cgggcaacgc
     1921 cgccagaggg agcgtgccga tgaccgtgag atcggtttga ttctcccccc ccgcggtccc
     1981 gcggatgtcc accgcgtaat cgccggagcg gaggtggagg gcgccgaggg tgagggcccg
     2041 ctggttccaa gcgagctgga ccgggtcgcg gctctcccag gccaggccgt ccccctggag
     2101 ctgcatccgc tcgaaattgg cctccccttg gctggcagcc aggtcgccgg ccgggccgcg
     2161 tacctggatg cgcccgctgg ctcgtagctg ccacgggctc ggggcggaca cccccgccgt
     2221 ctgaagcacc aaatccaggt ccgcgtcgcg aagcgacagc accgcctgtt ggctgcgatt
     2281 gggtggcggg ccaatggcgg cgtgcagctg ataggcgcca tcccggagcg tgaggtctac
     2341 atccagggcc ttccgctcga ggcgaaagct ggcggtcccg ggtccaaggg ggacctcccg
     2401 atagaacagg gcggtggccg tgacctggcc cagtgtacgc gtctcgtgca ggctgccctg
     2461 gaacttcagc tggaagctgg cccgtccggc gaggtcgctg cccgccaggg atccgactct
     2521 ggacagatcc gtgggcgtgc tggtcaggcc tccggaaagg atgccgtcgg ccgtcaattg
     2581 gatctccccg gtgagctgct cctccccgcg gcggagcgag atctgggtga tctccaccgc
     2641 gccctcccgg aagtggagca gggcgttgcc ttgctgccac gactcgtccc aggtccggac
     2701 attctccagt tggagtgtca cgtcgccgct caaggccttg ggggctcccg tgaggcggcc
     2761 actcgcctgg aaggccccct ggatctcgag gtcctccggc aggagggcga tcaggtcggc
     2821 cgtgcgcgcc gggttgagc
//
LOCUS       DBNGZC010000612         2410 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_54001, whole genome shotgun sequence.
ACCESSION   DBNGZC010000612 DBNGZC010000000
VERSION     DBNGZC010000612.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_54001"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /gene="hemC"
                     /locus_tag="AC16G0_10625"
     CDS             <1..592
                     /gene="hemC"
                     /locus_tag="AC16G0_10625"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HIR5100102.1"
                     /translation="PRGARVGTSSLRRQAQLLHLRPDFSVVGLRGNLDTRIRKLQEGG
                     LDAIVVAAAGVRRLGLAHLITETLPADILLPAIGQGALGIEVRAPSPERQAPSILQMM
                     TVLDHPETHIAVRAERALLTRLEGGCQVPIAAHATVSGEGINLRGLVASLDGKRLVRG
                     EVHGPTGSSEALGTNLAEDLLSRGAAEILQEIYARG"
     gene            596..2131
                     /gene="cobA"
                     /locus_tag="AC16G0_10630"
     CDS             596..2131
                     /gene="cobA"
                     /locus_tag="AC16G0_10630"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006423383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HIR5100103.1"
                     /translation="MSAEGSRKGMVFLIGAGPGDPGLLTLKGKRCLEGADVVVYDALA
                     NPRLLQHAKPGAELIYVGKRASQHTLPQEEIGRLLVERAKAGRVVARLKGGDPFIFGR
                     GGEEAEELAAACVPFEVVPGVTAAVAAPAYAGIPLTHRDFTSTVAFITGHEDPSKDES
                     GIAWEKIATGIGTLVFFMGVGQLPQIVGRLLRNGRSPATPAAVVRWGTRADQEVVVGT
                     LADLAEKCRGMKPPALIVVGEVVTLREKLRWFETKPLFGKRILVTRAREQASSFAQIL
                     EAAGAEVVEFPTITILPPENWAPLDTAIGRLREYQWVIFTSANGVRFFWERLEHAAKD
                     LRELFGITVCAIGPATAEALLRLGIRADIVPAEFRAEALVEAIGKDRVRGSRVLLARA
                     AEAREVIPEELARRGAQVDVVPAYRTVKNTSDAAEIRSMLREGKIHAVTFTSSSTVKH
                     FLDLVAEETEVLLKGVTVASIGPITAEAAAKRGVISHIMPESYTIPALAEAIVRHFQP
                     ETR"
BASE COUNT          424 a          713 c          855 g          418 t
ORIGIN      
        1 cccccgcggc gcgcgggtgg gcacgagcag cctgcggcgc caggcgcagc tcctccatct
       61 tcggcctgat ttttcggtcg tggggctccg gggaaacctg gacacccgaa tccgaaagct
      121 tcaggaggga ggcttggacg ccatcgtggt ggcggcggcc ggggtgaggc gactgggtct
      181 cgcgcatctg atcaccgaga cgctacccgc cgacatcctc ctgccggcca tcgggcaggg
      241 ggcattgggg atcgaggtcc gagccccgag ccccgagcgc caagcgccaa gtatccttca
      301 gatgatgact gtgctggatc acccggagac gcacatagcc gtccgggcag agcgggcgct
      361 gctcaccagg cttgagggcg ggtgccaggt gccaatcgcg gcccatgcga cggtctcagg
      421 ggagggcatc aatctgcgag gtctggtcgc cagcctggac ggcaagagac tggtgcgggg
      481 tgaggtgcac ggacccacgg ggagttccga ggcgctggga acgaacctgg cggaggacct
      541 cttgtcacgc ggggccgcgg agatccttca ggagatctat gcgcgggggt gaggcgtgag
      601 cgccgagggc agcaggaagg gtatggtctt cctcatcggg gcagggccgg gcgatccagg
      661 cctcctcacc ctcaagggga agcgctgcct agagggggcg gacgtggtgg tgtacgacgc
      721 cctcgccaat cccaggctgc tccagcatgc gaagccgggg gcggagttga tctacgtggg
      781 gaagcgggcg agccagcaca cgctgccaca ggaggagatc ggtcgccttc tggtggaacg
      841 ggccaaggcc gggagggtgg tcgcgaggct caagggcggc gatcccttca tctttggccg
      901 cgggggcgag gaggcggagg agttggcggc ggcctgcgtc cccttcgagg tggtccctgg
      961 cgtgacggct gcggtggcgg cgcctgccta cgccgggatt ccgctgacgc atcgggactt
     1021 cacctccacc gtcgccttca tcaccgggca tgaagacccg tccaaggacg agagcggcat
     1081 cgcctgggag aagatcgcca ccgggatcgg cacattggtg ttcttcatgg gggtgggcca
     1141 gctcccccag attgtgggac ggctcctgcg gaatggccga tcgccggcca cgccggcggc
     1201 ggtcgtgcgg tgggggactc gggcggatca agaggtggtg gtcggaacgt tagccgacct
     1261 ggcagagaag tgccgaggga tgaagccccc agccctgatt gtcgtgggtg aggtcgtgac
     1321 cctgcgagag aagctccgct ggttcgagac gaagccgctg ttcggaaaga gaatcctggt
     1381 gacccgggcg cgggagcagg caagcagctt cgcccagatc ctggaggcag ccggggccga
     1441 ggtggtcgag tttccaacca tcacgatcct gccaccggag aactgggcgc ccctggacac
     1501 tgccatcggc cggcttcgcg agtatcaatg ggtcatcttc accagcgcca acggcgtccg
     1561 cttcttctgg gagcggttag agcacgcggc aaaggacctc cgtgagcttt tcggcatcac
     1621 agtctgcgcc atcggacctg ccacggccga ggcactgctg cgtctgggca tccgcgcgga
     1681 tatcgtccct gccgagttca gagccgaggc cttggttgaa gccatcggca aggatcgcgt
     1741 ccgcgggagc cgggtcctgc tggcccgcgc cgccgaggcc cgggaggtca tcccggagga
     1801 gttggcccgc cgcggcgccc aggtggatgt cgtgcccgct taccggacgg tgaagaacac
     1861 ctcggacgcg gcggagattc gcagtatgct tcgggaagga aaaattcatg cggtcacgtt
     1921 cactagttcg tccacggtga agcatttcct ggacttggtc gcagaggaga cagaagtgct
     1981 tctgaaaggg gtaacggtcg ccagtatcgg tccgatcacg gccgaggccg ccgccaaacg
     2041 tggtgttatt agccacatca tgccggaaag ttacactatc ccagctctgg ccgaggctat
     2101 tgtcaggcac ttccagccgg aaacccggta agcaaccccg gtcaagccgc tctgattttc
     2161 cccttttccc ttgacaagta ttgaatttta cagatatttt cggccatccg gatccatcta
     2221 gccggtgctg cggtctctgc ccagcacctt tttaatttct ggaatggaat ctgcacgcta
     2281 aatccaggtt ggccctttga aggggagaga cggcacgcca tggtagtttc ggtcttggat
     2341 ctgcagacac agtatcagcg gatacgccgg gagattgacg aagcggtccg cagggttctg
     2401 gagggcgggc
//
LOCUS       DBNGZC010000613         3005 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2496541, whole genome shotgun sequence.
ACCESSION   DBNGZC010000613 DBNGZC010000000
VERSION     DBNGZC010000613.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2496541"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1058
                     /locus_tag="AC16G0_10635"
     CDS             <1..1058
                     /locus_tag="AC16G0_10635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR5100104.1"
                     /translation="RLVAFSAAIVGHVRGGLAYVVVVTNVIMAGFSGTALADASATGS
                     VLIPAMKREGYHAEFAGAITAAAATIGPIIPPSIPMIIYAVVAEVSVGSLFLSGVIPG
                     LLMGLYLFVVVFFISRRRGYPVRVRSSLRELWQATRRAFWALMAPVIILGGIVTGVVT
                     ATEAGVLAVVYSLIVGFFIHSELRVRDLPRVFRDTAVGTATVMIIVGASGVMGWLVAN
                     LRIGEALVAGVTSLTQNPWVILLLLNVFFLIVGCLMDPLAGMLIFIPVILPLTRHVGI
                     DPVHFGLVVVLNLMIGLLTPPVGYLLFLSAAMADVSIDRIVREVWPLVLALLVVLAIS
                     TYWPGLVLWIPNMFQPK"
     gene            1277..1945
                     /locus_tag="AC16G0_10640"
     CDS             1277..1945
                     /locus_tag="AC16G0_10640"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR5100105.1"
                     /translation="MRIVDLTFPIKPHFRWKVATELRSSHAKGDLFQSTVLTVHCHAF
                     THVDAPLHFLPGGKDIAQVPVDQWIGEAAVVDLTHVPENGEVTGADLERCARHVRAGD
                     TVLLRTDWPRRVSVETERFWKDAPYTGNSACDWLIQRKVKAVGYDYPPDYSVRTMIFE
                     PQKRLTREENPTHHAFFPAGITVVEYLTNLDQIGVPRCRFVALPLPIEGADGSPVRAV
                     AIVE"
     gene            2194..>3005
                     /locus_tag="AC16G0_10645"
     CDS             2194..>3005
                     /locus_tag="AC16G0_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HIR5100106.1"
                     /translation="MKPLYQEIGVVNTREMFKKAMQGGYAIPAYNFNNLEQLQAIILG
                     CVESKSGVILQVSKGAREYANQTLLRYMAMGAVKMAREMGSNIPITLHLDHGDTFELC
                     KSCVEFGFSSVMIDGSHHSYEDNIKLTKQVVDFAHQCDVSVEGELGVLAGIEEHVVAE
                     KSSYTDPGQVQDFVGRTGVDSLAISIGTSHGAYKFKLKPGETVPPLRFDILKEIEKRL
                     PGFPIVLHGASSVLQKYVAMINEYGGKLEGASGVPEEQLREAAKSAVCKINI"
BASE COUNT          523 a          889 c          999 g          594 t
ORIGIN      
        1 agcggctcgt cgcgttttcc gcggcgattg tcgggcacgt tcgcgggggg ctggcttacg
       61 tggtggtcgt gacgaatgtg atcatggcgg gcttctcggg taccgccttg gccgatgcca
      121 gcgctacggg ttccgtattg atcccggcga tgaagcggga ggggtaccat gcggaattcg
      181 ccggggccat cacggcggcc gccgccacca tcgggccgat catcccgccc agcatcccga
      241 tgatcattta tgcggtggtg gccgaagtct cggtggggtc gctattcctc tcgggcgtta
      301 ttccgggcct cctgatgggc ctgtacctgt tcgtcgttgt cttcttcatc agccggcggc
      361 gcggataccc ggtgcgggtg cggagcagcc tgcgggagct ctggcaggca acgcggcgcg
      421 ccttctgggc cctgatggcg ccggtcatca tcctgggggg gatcgtcacc ggtgtggtga
      481 cagcgacgga ggccggggtc ctggcggtgg tctacagcct gatcgtgggc ttcttcatcc
      541 acagcgagct gcgcgtccgc gatctgcctc gggtcttccg ggacacggcc gtcggcacgg
      601 cgacggtgat gatcatcgtc ggggccagcg gcgtcatggg ctggctggtc gccaacctcc
      661 ggatcgggga ggccctggtg gcgggggtga cgtccctgac ccagaatccc tgggtcatcc
      721 tcctgcttct gaacgtgttc ttcctcatcg tgggttgcct gatggatccc ctggccggca
      781 tgctgatctt catccccgtg atcctcccgc tgaccaggca tgtcggtata gatccggtgc
      841 acttcgggct ggtcgtcgtc ctgaacctga tgatcgggct cttgacgccg ccggtggggt
      901 acctgctgtt cctgtcggca gccatggcgg acgtctcgat cgatcggatc gtccgcgagg
      961 tctggccgct ggtgctggcg ctcctggtgg tcctggccat ctccacgtat tggccgggtc
     1021 tggtcctatg gatcccgaac atgtttcaac ccaaatgact cagccttagc gcagatttcg
     1081 cggacagcgt agaggatgac gaagagcttc ctgcgaattc aggcatgacg ggtcttggga
     1141 cgcggtgcat ttttcccggt ctgaatctgg aaagagccca tcccgtatgg ctctgcgatc
     1201 tttgcggtga aagggttggc ctgtattgac aggggcatct gcggcccacc gcagggcgcc
     1261 cggatggggg cctggcatgc gcatcgtgga tctcacattt ccaatcaagc cccactttcg
     1321 ctggaaggtg gcgacggagc ttcgctcgtc gcatgccaag ggggacctgt tccagagcac
     1381 ggtcctgacc gttcactgcc acgcgttcac ccatgtggac gcccccctgc acttcttgcc
     1441 gggtggcaag gatattgccc aggtgcccgt ggaccagtgg attggagagg cggctgtcgt
     1501 ggacctcacc catgttcccg agaacggcga ggtgacgggg gcggacctgg agaggtgcgc
     1561 ccgacacgtc cgggccggag atacggtcct gctccggacg gactggccaa ggcgggtcag
     1621 cgtggaaacc gagcggttct ggaaagatgc gccctacacg ggcaacagtg cctgtgactg
     1681 gctgatccag cgaaaggtca aagccgtggg gtacgactac ccccccgact attctgtccg
     1741 gacgatgatc ttcgagcccc agaagaggct gacgcgcgaa gagaacccga cccaccacgc
     1801 ctttttcccg gccggcataa ccgtggtcga gtacctgacc aacctggatc agatcggggt
     1861 gccgcgctgc cgcttcgtcg ccctgccgct ccccatcgag ggggctgacg gatctcccgt
     1921 tcgggcggtg gcgatcgtgg agtgaccgtc gggaaagcaa ttcaaccgcg ggcttcaggg
     1981 cttccggacg gaagcactct cggagaaatc tgcatagact atggttgatc gggaggttcg
     2041 aggattcgta catagatacg gaccagccac ggattgctat tgtccgctgg ggcagaggga
     2101 ggcatagtct atgtaggctt tgcgccgaat ctcacagagt tcaaggcgtg gattctaccg
     2161 gatcctgctg agatccgcag aaaggagaag gaaatgaagc cgctgtatca ggagattggt
     2221 gtcgtcaaca cgagagagat gttcaagaaa gccatgcaag gcgggtacgc catccccgcg
     2281 tacaacttca ataatctgga gcagctccag gccatcatcc tgggttgcgt cgagagcaaa
     2341 tcgggcgtca tcctccaggt gtcgaagggc gccagggagt acgccaacca gacgctgctc
     2401 cggtacatgg ccatgggcgc agtgaagatg gcgcgcgaga tggggagcaa catcccgatc
     2461 accctccatc tcgaccacgg cgataccttc gagctgtgca agtcctgcgt ggagtttggc
     2521 ttctcctccg tcatgattga cggctcccac cattcgtacg aggacaacat caagcttacc
     2581 aagcaggtcg tggattttgc ccaccagtgc gatgtgagcg tcgagggcga gctgggcgtc
     2641 ctggcgggca tcgaggagca cgtcgtggcg gagaagtcca gttacaccga ccccggtcag
     2701 gtccaggact tcgtggggag aaccggggtg gacagcctgg ccatctccat cggcacctcc
     2761 catggggcct acaagttcaa gctgaagccg ggcgagaccg tgccgcccct gcgcttcgac
     2821 atcctgaagg agatcgagaa gcggctgccg gggttcccca tcgtcctgca cggggcctcg
     2881 tcggtcctgc aaaagtacgt ggccatgatc aacgagtacg gcgggaagct cgagggcgcc
     2941 tccggcgtgc ccgaggaaca gttgcgtgag gcggccaagt cggcggtctg caagatcaac
     3001 atcga
//
LOCUS       DBNGZC010000614         4938 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1029143, whole genome shotgun sequence.
ACCESSION   DBNGZC010000614 DBNGZC010000000
VERSION     DBNGZC010000614.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1029143"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2351)
                     /locus_tag="AC16G0_10650"
     CDS             complement(<1..2351)
                     /locus_tag="AC16G0_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit alpha/beta"
                     /protein_id="HIR5100107.1"
                     /translation="MVKKLLLVPEEVRRAETINLGTIPVNAYKRTLADEIAQRNLTAA
                     DAVRIYRDMCLVRQFEEMLGELKRVRQYRGVSYNHQGPAHLSIGQEAAAVGEAFLLDV
                     ADHIYGSHRCHGEILAKGLAAIHRLDETTLQRIMEEYLGGGILRVAERRSAATGKERA
                     IDFLLYGLLAEVFGRASGFNRGLGGSMHAFFPPFGIYPNNAIVGGSADIAVGAALFKK
                     VQREAGIVVVNIGDASLGCGPVWEALHFSTMGQFWTLWEPEYRGGPPLIFAIMDNFYG
                     MGGQTRGETMGFNRLATLAAGLNPWNLHAESVDGNNPLAVIDAFRRKRAVLAAGEGPV
                     LLDILTYRLSGHSPSDAGSYRTREEVQLWQAVDPIREYRGQLLTAGAAGEAELDALAA
                     YAGEKVLTACRLAVDLTTSPRLTGEAIAGAMFPQRAKDRLPGMTPGATCKPLEENSRV
                     LAIAKKSRSGIDPASGKKLSEGKAVSFRDALFEAILARVYQDGRLIIYGEENRDWDGA
                     FAVYRGLTESLPYHRFFNAPISEGAIVGTAVGYAMEGGRALVELMYCDFLGRAGDEIF
                     NQLAKWQAMSAGVLRMPVVLRVSVGSKYGAQHSQDWSAMTAHMPGLQVVFPATPYDAK
                     GLMTTALQGSHPVVFFESQRLYGVTEWFRPEVPASDYAIPIGLPEVKRQGTDLTVLSI
                     GSTLYRVMEAADRLQQEFGVSAEVVDARSLVPFDYTPVLDSVRKTGHILLTSDACERG
                     SFPQVLAATIQELAFDALDAPAVVVGARNWITPAAEMEETFFP"
     gene            complement(2572..4038)
                     /gene="glgA"
                     /locus_tag="AC16G0_10655"
     CDS             complement(2572..4038)
                     /gene="glgA"
                     /locus_tag="AC16G0_10655"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - alpha-1,4-glucan
                     glucosyltransferase (UDP-glucose donor) activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HIR5100108.1"
                     /translation="MRILLAASEVAPFAKTGGLADVAGALPKAIHALGHDIRVVLPKY
                     RMVDAAKHGLRQIRASFPVPIAGRAENAALWQGAIGPVPVYFIQQEEYYDREGLYQLK
                     GVDYPDNAERFAFFSRAVLELTRILDFHPAVYHCNDWQTGLIPTYLRTTFEADPEFRT
                     AGVLFTVHNLGYQGLFSPEVLAVANLDHKVFNPFGIEFYGKVNFLKAGLVFSDLINTV
                     SLKYSQEIQTEEFGHGLEGVLRARAKDVHGILNGIDYEEWHPSRDAFITAHFTRDNLA
                     GKAACKADLQRIFGLPERPDLPLLAVISRLAPQKGMDIVAEAMNAMLALDTQFVLLGT
                     GDPDLHEAFEAAKAKHPKRVGLRLGFDVGLSHKIEAGSDMFLMPSRYEPCGLNQMYSL
                     AYGTIPVVRATGGLDDTIQPFDPETGTGNGFKFPGTTPEDLLTTVRAAVAVYRRRDQW
                     GRLVRNAMAFDFSWDRSAREYEKLYQEIAARHAGKDQP"
     gene            complement(4043..>4938)
                     /locus_tag="AC16G0_10660"
     CDS             complement(4043..>4938)
                     /locus_tag="AC16G0_10660"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5100109.1"
                     /translation="GLWPEACWDRGPVIDVSVHDVSLSRKIPALLGRPTATPPPRDPR
                     SRQGEEVKLSELIEDMDRAGVKTSLVVIHEEVDEFFSLAARHPGRLFGLAYFDSLSPP
                     RGLERVRSLWNDHRDLILGVRTAMPMLHQDPRLREFVPLYQYCLERDLPLQFSIGGNP
                     QEAATRPTPFGVLAVSYPRLRIVCLHAGGPWHPELPGYLRRFPNLYAAITGSLGSDDE
                     GDGGSRTSRELLRQAGSRQLVFGSNWLGRDPAYRHAVERMRSLPWWQRRNLCWRTAVR
                     VYGARILGDPATPMATSPTTR"
BASE COUNT          854 a         1603 c         1642 g          839 t
ORIGIN      
        1 tgcgggaaga acgtctcctc catctcggcg gctggggtga tccagttccg ggcgcccacc
       61 acgacggccg gcgcgtccaa ggcgtcgaag gccagctcct gtatggtcgc ggccaggacc
      121 tgaggaaagc tgcctcgctc gcaggcatcc gaggtcagga ggatgtgacc ggtctttcgc
      181 accgagtcga ggacgggggt gtagtcgaag gggaccaggc tgcgggcgtc cacgacctcg
      241 gcggagacac cgaactcctg ctggaggcgg tccgcggcct ccatgacccg gtacagcgtc
      301 gacccgatgc tgaggacggt gagatccgtc ccctgccgct tcacctccgg cagaccaatg
      361 gggatggcgt agtcgctggc cggcacctcc ggccggaacc actcggtgac cccgtagagc
      421 cgctggctct cgaagaatac gacaggatga ctaccctgga gggccgtggt catgagcccc
      481 ttggcatcat agggggtggc ggggaacacc acctggaggc cgggcatgtg ggccgtcatg
      541 gcgctccagt cctgggagtg ctgcgcgcca tacttgctgc cgacgctcac ccgcaagacc
      601 acgggcatcc ggaggacacc cgcggacatg gcctgccact tggcgagctg gttgaagatc
      661 tcgtccccgg cccgtcccag gaagtcacag tacatcagct ccaccagggc cctcccccct
      721 tccatggcat agccgacggc ggtgcccacg atggcgccct cggagatggg ggcgttgaag
      781 aagcggtggt agggcaggct ctcggtcagc ccccggtaca cggcgaaggc cccgtcccag
      841 tcccggttct cctccccgta gatgatgagc cggccgtcct ggtacacccg ggccaggatg
      901 gcctcgaaga gggcgtcgcg gaaggacacg gccttccctt cggagagctt cttgccgctg
      961 gccggatcga ttcccgaccg gctcttcttg gcgatggcca ggacgcggct gttctcctcc
     1021 aacggcttgc aggtcgcccc gggggtcatc ccaggtaggc ggtccttcgc ccgctgcggg
     1081 aacatggcgc ctgcgatggc ctcccccgtg agtcgcggag aggtcgtgag gtccaccgcc
     1141 agcctgcagg cggtgaggac cttctccccg gcgtaggccg ctagggcatc cagttctgcc
     1201 tcgcctgccg cacccgccgt gaggagctgg ccgcggtact cccgaatcgg gtccacggcc
     1261 tgccacagtt ggacctcctc gcgggtacgg taggagccgg cgtcgctcgg cgagtggccg
     1321 ctcaggcggt aggtcaggat atccaggagc acgggacctt cgccggcggc gaggaccgcg
     1381 cgctttcgcc ggaaagcgtc gatgacggcc agcgggttat tgccgtccac gctctcggcg
     1441 tggaggttcc aggggttcag gccggcggcc agcgtcgcaa gccggttgaa gcccatggtc
     1501 tcaccgcggg tctggccgcc catcccgtag aagttgtcca tgatggcgaa gatcaggggt
     1561 gggcccccgc ggtactcggg ctcccagagg gtccagaact ggcccatggt cgagaagtga
     1621 agggcctccc acaccgggcc acagccgagg gaggcgtccc cgatgttcac gacgacgatg
     1681 cctgcctccc gctgaacctt cttgaacagc gcggcgccca ccgcgatgtc cgcgcttccc
     1741 ccgacgatgg cgttgttggg atagatcccg aagggaggga agaaggcgtg catggagccg
     1801 cccaggccgc ggttgaatcc cgaggcgcgc ccgaagacct cggccaggag gccgtagagc
     1861 aggaagtcta tcgcccgctc cttgccggtc gcggcggacc gccgctccgc cacccggagg
     1921 atgccgccgc cgagatactc ctccatgatc cgctgcagcg tcgtctcgtc caggcggtga
     1981 atggcggcga gccccttggc caggatttcc ccatggcacc ggtggctgcc atagatatgg
     2041 tccgcgacat ccaggaggaa ggcttcgccc accgccgcgg cctcctggcc gattgacagg
     2101 tgggccggac cctggtggtt gtagctgacg cctctgtact gccgcacccg cttgagctcg
     2161 ccgagcatct cctcgaactg gcggacgagg cacatgtcgc gatagatgcg gaccgcgtcc
     2221 gccgccgtga gattcctctg ggcgatctcg tcggccaggg tccgcttgta ggcgttgacc
     2281 gggatcgttc ccaggttgat ggtttccgcc cgccgcacct cctcggggac gagcaggagt
     2341 ttctttacca tgggacgaga actccttcta gcctcatccg aaactcagtt atcaccaagg
     2401 cacagcgaca ccaaaacgag catagaccgg catgaatctg gcggcgagca ctctgattga
     2461 actcgcagcc aatgactgcg aaggtcccag gcgggcattg gtatgtttca gtgccgagtg
     2521 tcagaatgct tgcccctgtc ttggtgcctg gctgatgtgc gttgtcccgg gttagggttg
     2581 gtcctttcct gcatgacggg cggcgatctc ctgatacagc ttctcgtact ccctggcgga
     2641 gcgatcccag gaaaagtcga aggccatggc atttcgcacg aggcgccccc attgatcccg
     2701 tcggcggtag actgccacgg cggcccgcac cgtcgtcagg agatcctccg gggtcgtgcc
     2761 cgggaacttg aagccgttgc ccgtacccgt ctcggggtcg aagggttgga tggtgtcgtc
     2821 caggccaccc gtggcccgaa ccacggggat ggtcccatag gccagactgt acatctggtt
     2881 caggccgcag ggctcgtacc gcgacggcat gaggaacatg tccgagcccg cctcgatctt
     2941 gtgggacagg cccacatcga agccaagcct cagaccaacc cgctttgggt gcttcgcctt
     3001 ggcggcctcg aaggcctcgt ggaggtcggg atctcctgtc cccagaagga cgaattgcgt
     3061 gtccagggcc agcatggcgt tcatggcctc ggccacaatg tccatgccct tctggggggc
     3121 caggcgagag atcacggcca ggaggggaag atcgggccgc tcgggcaagc cgaagatgcg
     3181 ctggagatcc gccttgcatg ccgcctttcc agccaggttg tcgcgggtaa agtgggcggt
     3241 gataaaggcg tcgcggctgg ggtgccactc ctcgtagtcg atgccgttca ggatcccgtg
     3301 gacatctttg gcccgggcgc gcaagacgcc ctccagcccg tggccgaact cctcggtttg
     3361 gatctcctgg ctgtacttca ggctcacggt gttgatgagg tccgagaaga ccagccccgc
     3421 cttcaggaag ttcaccttgc cgtaaaattc aatcccgaaa ggattgaaga ccttgtgatc
     3481 gagattcgcg accgccagga cctcggggct gaacagcccc tggtatccca gattgtggac
     3541 cgtgaagaga acgccggccg tccggaactc cgggtctgcc tcgaaggtcg tgcgcagata
     3601 cgtcgggatc agcccggtct gccagtcgtt gcagtggtac acggcgggat gaaagtccag
     3661 gatccgcgtc aattccagca ccgcccggga gaagaaggcg aagcgctctg cattgtcagg
     3721 ataatccaca cccttgagct ggtacaggcc ctcacggtcg tagtactcct cctgctggat
     3781 gaaatacacg ggcacggggc cgatggcccc ttgccacagg gcggcgttct ccgcccgccc
     3841 ggcgatgggg accgggaaag aggcgcggat ctggcgaagg ccgtgtttgg ctgcatccac
     3901 catcctgtac ttgggcagaa ccacccggat gtcgtgcccc agggcatgga tggctttggg
     3961 gagcgccccg gccacatccg caagtccacc ggtcttggcg aaaggggcga cttctgaagc
     4021 cgcgagcagg atccgcatga tgtcacctcg tggtaggaga agttgccatc ggagtcgctg
     4081 gatctccgag gatgcgcgca ccatacaccc gcactgccgt gcgccagcag aggttccgcc
     4141 gctgccacca cgggaggctc cgcatgcgct ccacggcatg acggtacgcc ggatcccggc
     4201 cgagccagtt gctgccgaac accagttggc ggctgccggc ttggcgtagc agctcccgag
     4261 atgtccggga gccgccgtcc ccttcgtcgt ccgatccgag cgacccagtg atcgcagcgt
     4321 agagattcgg gaagcgccgg agatatccgg gaagctctgg atgccagggc ccgcccgcat
     4381 gaagacagac gatcctgagc cgagggtagg acacggccag gacgccgaag ggcgtgggcc
     4441 gggtggccgc ttcctgagga ttcccgccga tggagaattg aagcgggagg tcccgctcca
     4501 ggcaatactg gtacagcggg acaaattccc gcagccgggg atcctggtga agcatcggca
     4561 tcgccgtccg gacccccagg atcagatccc ggtggtcatt ccacagggac cgcacccgct
     4621 ctagcccccg ggggggcgaa agcgaatcga aataggccag accgaagagc cggccagggt
     4681 gacgcgcagc caggctgaag aattcgtcca cctcctcatg gatcacgacc aggctcgttt
     4741 tcacgccggc ccgatccatg tcttcgatca attcggagag cttgacctct tccccctgac
     4801 gactccgcgg gtccctgggc ggtggagtcg cggtgggacg cccgaggagg gcgggtatct
     4861 tccgggacag agaaacgtcg tggacggaga cgtctatcac cggccctcga tcccaacagg
     4921 cctccggcca gagcccgc
//
LOCUS       DBNGZC010000615         2763 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1323772, whole genome shotgun sequence.
ACCESSION   DBNGZC010000615 DBNGZC010000000
VERSION     DBNGZC010000615.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1323772"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(341..760)
                     /locus_tag="AC16G0_10665"
     CDS             complement(341..760)
                     /locus_tag="AC16G0_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5100110.1"
                     /translation="MAKKRVLLVGDDAVIRKAIQQRLERSGYIYAGAANGTQGLPLAE
                     RLHPDVILLDIRMPGLYSYEVCRRLKENPATAAIPVIFLTAVHETSLERLAFEAGAVA
                     CVTKPFHPEALVALIEASIVSAELRAKRKRKGRASAR"
     gene            complement(1433..2416)
                     /locus_tag="AC16G0_10670"
     CDS             complement(1433..2416)
                     /locus_tag="AC16G0_10670"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR5100111.1"
                     /translation="MAIDPYQTTERVNRAVGSGVILDKEGIVLTNSHVVFGRQAITVT
                     LDDGTTVPGEVVGADPVFDVAVLRIPRPASGSLPVAVPGSSAHLRVGEEVLAIGNPLG
                     LDQTLTRGVVSALNRILPDTPFAFMEPLIQTDASINPGNSGGPLLNRCGEVIGITTAI
                     LPDAQNIGFAIPIDLVKTALPSLLEKGRIIRPWLGIQGHLVGPSLKDLLRIPLTDGLL
                     VEVVEPGSPAERVGIRGGNLDVVIGGHALLLGGDIVTRVNGRPVDTPERLTTEMKRLQ
                     VGDTLHLTLYRDGETKDVEFVLPERPVLPQDLLAHPSVAPALRTRSLRLSH"
BASE COUNT          501 a          876 c          880 g          506 t
ORIGIN      
        1 cgccggatcg gtggtgaata gcacccggta gccatgggcc tccagcgcaa aggtgcatac
       61 gccaagcacg agccggtcgt catcgataca gaggatcgtc tgacgggagc ctcccgcctg
      121 ggtctcaccg ctcatcgcgt cttctccgcc ttacccccgc gcctaagacg tcgcctgtcg
      181 ctctccctcc ccagtgccga tccagcgtcg ccttcgcctc ccacaaggtc tccacgctgg
      241 tactaaccac aaccacaaag ccgcggaccg ccatgaccta attccgcttg caccggctca
      301 tggccgcccg gcaccgatct gagcaatgtg acgcggaagt tcagcgcgca ctcgccctgc
      361 cctttctctt gcgtttcgcc cggagttccg cgctcacgat gctggcctcg atcagggcca
      421 cgagggcctc ggggtggaag ggtttcgtga cacaggcgac ggcccccgcc tcaaaggcga
      481 gccgttccaa cgaggtctcg tggacggccg tgagaaagat aaccggaatg gctgcggtcg
      541 cggggttctc cttcagccgt cgacagacct cgtagctata gagtcccggc attcgaatat
      601 ccagcaggat cacatccggg tgcagccgct ctgctagagg gaggccctgg gtcccattgg
      661 ccgccccggc gtatatgtag ccggaccttt cgagtcgctg ctgtatcgct ttgcggataa
      721 cggcatcgtc acccacaagg agcacgcgct tctttgccat ggtcctcccg ccctaggctg
      781 ctgtcttgga accccggagg gcccgcatcc tggtcaacga ccgcatgagc tgctcctcca
      841 ccaaggcgag atcaccctcc tggtggcccc atcatgccgg gcctcggccc ttgtcggagc
      901 atcgcgtccg ctcgcgctcc gtgaaggcgt tcccgcagat cgggcagttt ccgtaagagc
      961 ctaccacact ggtccgtgac gcgctagttg ccgcacattc ggttgtggga gtggcgggag
     1021 ctatgtctgt gcaatcaatc atgtgcagca tctcccaggt gagctacaga agaaccccag
     1081 gcaatgcgcc agaggcaccc gatcgcgccg gagagcgctc gggaggcgag agttttacta
     1141 ttcttcggct gggtgactat cctggatagg ggtaccgagg agtcatcacc agcgtctcgt
     1201 cttctaggat ggcgttaacc ttgtagagat cctgtcgtcc gcgtctttct cgtccggctc
     1261 actacctggg gtgggtgacc aaccgctcga aaattggaga gggacccgct ggaccgggcg
     1321 cgggcgcgct cggcctgggg gttgagggga tgagagcggc aggggagtgg gagatgcttt
     1381 cgcagcaaaa ttccccgatt gtcctcatca ccctcgatgt ctcaatccgg catcagtgag
     1441 agagccgtaa cgagcgtgtg cggagggccg gggccacgga gggatgggcg aggaggtctt
     1501 gggggaggac cgggcgctca gggagaacga actcaacatc cttggtctcg ccgtctcgat
     1561 agagcgtcag gtgcagcgtg tctccgacct gcaagcgctt catctccgtc gtgagtcttt
     1621 ccggggtgtc caccggccgt ccgttgaccc gggtgacgat atcgcctccg aggagcagag
     1681 catgcccgcc gatgacgaca tccagattgc cgcctcggat ccccacccgc tctgccggac
     1741 ttcctggttc cacgacctcg acgaggagcc catctgtcag ggggatgcgc aggaggtcct
     1801 tcagggacgg gccaacgagg tgcccttgga tcccgagcca cggacggatg attcgcccct
     1861 tctcgagcag cgaggggagg gcggtcttga cgaggtcaat ggggatggca aagccgatat
     1921 tctgggcgtc ggggaggatg gcggtggtga tgccgatgac ctccccacag cggttcagga
     1981 gcgggccacc cgagttgccc ggattgatcg aggcgtcggt ctgaatcagg ggctccatga
     2041 acgcgaaggg cgtatcgggt aggatccggt tgagggccga gaccaccccg cgcgtcaatg
     2101 tctgatcgag cccaagggga ttcccaatgg ccagcacctc ctccccgacc cgcagatgcg
     2161 ccgaactccc gggcacggcg accggcagtg atccggaagc cgggcgagga atgcgcagga
     2221 cggccacgtc gaagaccgga tcggccccta ccacttcccc cgggaccgtt gtgccgtcgt
     2281 cgagtgtcac cgtgatcgcc tggcggccga agacgacatg ggagtttgta aggacgatgc
     2341 cctctttatc cagaatcacc cccgacccga ccgcccggtt cacacgctcc gtggtctggt
     2401 acgggtcgat cgccatggaa gcaatcgaaa ccactgcggg ggagacccgc tcgtagatct
     2461 caggaagcgc ttgactgcac aatgcccctt ccgctcgcgc ttggctcggg ggactggtaa
     2521 agagcacaca gaggagcagg gaaccaaggg tagggaggaa accgagatgg ccgaggcctt
     2581 tccggatacc ggcgtcacgt tgctgcgcgg gcatgggcat tctcccggta atgcgggttc
     2641 agcgtcattt ggacgattcc ttcagacgcc ctgagggctg gagacatggc actggtcccc
     2701 actccgccct gggcacctta tactaagcgg tccggaagta gggctatata tccgcccgga
     2761 ccg
//
LOCUS       DBNGZC010000616         7884 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_248352, whole genome shotgun sequence.
ACCESSION   DBNGZC010000616 DBNGZC010000000
VERSION     DBNGZC010000616.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7884)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7884)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7884
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_248352"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            807..1937
                     /locus_tag="AC16G0_10675"
     CDS             807..1937
                     /locus_tag="AC16G0_10675"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HIR5100112.1"
                     /translation="MTTFNILPKEQCRWDGVAIGEVMIRFDPIDVPFERARTCRIWHG
                     GGETNVAEGLATCFGLRATVLTALVDDGFGRNIQNQFREAGVDTSHILWFSNSGKGKY
                     ATDKKGTLMNGVNATFRGKGVIPSKTEYYRAHTPIREVGAGDYDFDRLFGQEKVRWAH
                     TGGIYSLLSPKTAETAVEYLKKAGQHGTFRSTDLNYRANVEPNKERARSINRQIVPHV
                     EFLVGNQSDFDDALGYTGDVPSNAGMEQFLECYVTLLRKVAKDFPNLKLIGTQLRGAH
                     SADRIDWGAVLYDVAEDKLYQGAIRRNVEIADRTGGGDSFASAVIAALLQGKPLDEAV
                     EWGAAHGILVQETPGDTTMVTQAMVEAEVKRAKKGGGVVALR"
     gene            2051..2818
                     /locus_tag="AC16G0_10680"
     CDS             2051..2818
                     /locus_tag="AC16G0_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100113.1"
                     /translation="MVEGYTRDPKRVDRALREKGVVVVMGRDHARSPQDVINTVRAIY
                     EAGYIAEVTFRIPEEILKEAMAELRKRRVESVKARPSDPMLLGVGSVINPRELESAIE
                     MGFDMIVGPDAGMGGSRERIEFVRIVRGAKAFGVPGSFSPSEFAYFLEREDGLEPDGI
                     KIFNASIYGPSGIGALLAPYQRERHNGKMIMPTGGVNVKTGAAFQEQISKRGFYPVLG
                     MSAPLELVTERKKAGDPDTLKESLQKFKAEFKPYKPA"
     gene            2968..3636
                     /locus_tag="AC16G0_10685"
     CDS             2968..3636
                     /locus_tag="AC16G0_10685"
                     /inference="COORDINATES: protein motif:HMM:NF015682.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HIR5100114.1"
                     /translation="MSSSTLSPAELSQRYKALYTGAVSDILDSLGLRQQALPYPIMPL
                     SLDMVVAGPAFTGQGYPVEDISNDDSHMRIRMLESIKPGTVSVWSSAGHFESAHWGEI
                     MSNAARERGCTGAVVDGGLRDTRFVLQMGFPVFCRFRCSASSIGRWEIKEWMVPIQIG
                     RTAIRPDDFVFGDVDGVVVVPKELTVEVLLKTEEMVGRENSMRADLAKGITVSEVYRK
                     HGKF"
     gene            3882..4424
                     /locus_tag="AC16G0_10690"
     CDS             3882..4424
                     /locus_tag="AC16G0_10690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5100115.1"
                     /translation="MPETDEALFEKVQSGRQEAFATLVERYQRRGYAVALRLLGRGQD
                     AEDAVQQAFLRLYESRAAFDSRCRLSTWFYRILTNTCVDELRRRRPTLPLPETEMPAE
                     DAPDKDLEAAERHRLLHRALAEVPVEARIALTLYYGDGRSYGEIGAIRGISVNTVKTH
                     LRRGRLALRKALRAQGLASS"
     gene            4625..5272
                     /locus_tag="AC16G0_10695"
     CDS             4625..5272
                     /locus_tag="AC16G0_10695"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR5100116.1"
                     /translation="MEQIELTLDAAEPSDLLQRLDLAASSAGITHVSLRRRRGVSPAV
                     GPAGTIGNLLAQARRELEEYFAGRRTFFTVPVDLSHVPAFEREALEVAARIPYGEVRS
                     YKWIAEQLGKPDAARAVGGAMAGNPVPIIVPCHRVVKTDGGLGGYSFGLMRKEALLNL
                     ERSTTPYIGCLTTKFFCRRGCHAEQRIGEGNRIKFFSPADAQASGYRPCSVCQPA"
     gene            complement(5369..5857)
                     /locus_tag="AC16G0_10700"
     CDS             complement(5369..5857)
                     /locus_tag="AC16G0_10700"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5100117.1"
                     /translation="MTPKQFIEYAESIYRPTAKLVSLAPTNKLDWKPAKGDYMSLGQL
                     LHHLSTCPGVFVAAVNNAFPSPEAFQKFLEEDLKNTKTPEIAAREASRGWDESKAALL
                     SVSDGDFQAKMVSVPWGPPMPLWRVSLGMADHWVNHKYQLFFYLKLLGLPVNTMTLYA
                     GA"
     gene            complement(5972..7231)
                     /locus_tag="AC16G0_10705"
     CDS             complement(5972..7231)
                     /locus_tag="AC16G0_10705"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008361315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR5100118.1"
                     /translation="MEKILDCVHCGFCLPTCPTYLVLGNEMDSPRGRIYLMRAAGEGR
                     VEIGDSFQKHMSLCLVCRACETACPSGVDFGHLMEAARGQVVRKYTHSFSDRLFRKFI
                     LDTFTDPSRLRILLAPMRLYQKLGIQHLLRSTGLLKLLGRWGAMEGLLPALPDPGLVT
                     HFPEVTPAVGTRRGRVALLLGCAQHAFFPDVNLATARVLAANGFEVVAPRKQGCCGSL
                     LVHEGEREKGKALARKAIDVFEAAGADLVAINAAGCGSVMKDYGDLLKDDPRYAGRAA
                     AFSQRVRDVSQILAEAGPVGTLHPVKLRVAYHDACHLAHGQRVRVEPRTLLAAIPGLE
                     LVPLRDADFCCGSAGVYNLLHPELSREFLERKLDRLAETGAQVVAAGNPGCLLQIAMG
                     LRPRGLPMRAVHTVEVLDWSYRGITPL"
     gene            complement(7381..>7884)
                     /locus_tag="AC16G0_10710"
     CDS             complement(7381..>7884)
                     /locus_tag="AC16G0_10710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5100119.1"
                     /translation="QLEQARGMGRQAGGDDGLMLEGEEHEQFWGAVRDFAPGNGGPAM
                     VLKAGVLISEVAEAMRQGEEVAAKQGLHVSTISEAGTGIVRYYLQADSAASDSIVRLT
                     GTVAPLRAFAIEAKGSLVVLQAPPEVKAAVDVWGPVGDTLAQMRGLKDQFDPGRVLNP
                     GRFVGGL"
BASE COUNT         1512 a         2478 c         2559 g         1335 t
ORIGIN      
        1 tatgagagac caccgacgat cagtggctca tgctaggtag ttcccggcct gggcaaccct
       61 ccaaccgtgt aagaattaca cgggtaacag atcagatttg cacagtgaag gagaccttat
      121 ccgaggagag gcccccggag accaatgttt tcgacgcaag aatcccggat ggcccgggca
      181 ctgcgcggaa caacccacac gaagaagcga gggcgggcga cttggtccct atgggagata
      241 ggcatgtaaa catgtaaagg ggtcagttca agcaagagac ggcagtcaag aatctgattg
      301 gccggataga tccagagagc aggatgcgca gcacgattga ggttagccgt catcgagagc
      361 tagcggcggc acgagtatcc acccggaaga cgtcatcagc tatcgaccga cgcctggttt
      421 gacactcatg gaggtttcac atatactcct cgccggccaa ggggggtggg ttcggacgaa
      481 gccggcctcg ggatgacttg cagtcccgtg ggagccgatt cacggcactg cgtgctccct
      541 ggccacgggg acccagcagg gcgggcgggc cggatctctc gctttcatcg attgagtcat
      601 gggcccctgg gcagcccgct gtcacagacc gatcactcat ccgccatgga catcatcgtg
      661 gcccatcctc ggcgcgcccc ggcaggcccg caggaccccc gtccccaacc ttcacccgtg
      721 tcctggtccc tgcacgacag atgcgggcaa gaggccgatt gccctgaaca ccgcatcacc
      781 aatccgaagg gaggctctga cgtacaatga ctacattcaa cattctacct aaagagcagt
      841 gtcgctggga cggagtggcc atcggcgagg tgatgattcg gttcgacccc atagacgtgc
      901 cattcgagcg ggctcggacc tgccgcatct ggcacggtgg gggcgagacc aacgtggccg
      961 aggggttggc gacctgcttc ggcctgcgcg ccacggtcct gacagccctg gtggacgacg
     1021 gcttcggtcg caacatccag aaccagttcc gggaagccgg ggtggatacc agccatatcc
     1081 tctggttcag caacagcggc aaggggaagt acgccacgga caagaagggc accttgatga
     1141 acggcgtcaa cgccaccttc cgcggcaagg gcgtcatccc atccaagacg gaatactatc
     1201 gcgcccacac ccccatccgt gaggtgggcg ccggggatta cgacttcgac cggctcttcg
     1261 gccaagagaa ggtgcgctgg gctcatacgg gcgggatcta cagccttctc tcccccaaga
     1321 ccgccgagac cgctgtagag tacctgaaga aggccgggca gcatggcacc ttccgttcca
     1381 cggacctaaa ttaccgggcc aatgtggagc caaacaagga gcgcgcccgg agcatcaacc
     1441 gccagatcgt acctcacgtg gagttcctgg tcgggaacca gagcgacttc gacgacgccc
     1501 tgggctatac cggggacgtg cccagcaacg ccggcatgga gcagttcctg gagtgttacg
     1561 tcaccttact ccggaaggta gccaaggatt tcccgaacct caagctcatc ggcacgcagc
     1621 ttcggggcgc ccactcggcc gatcggatcg attggggcgc ggtgctgtac gatgtcgccg
     1681 aggataaact gtaccagggg gccatccgcc gcaatgtgga gatcgcggac cgtaccggcg
     1741 gcggggactc cttcgcgtcc gccgtcatcg cggcgctttt gcagggcaag cccctggacg
     1801 aggccgtgga atggggcgcc gcccacggga tcctggtcca ggaaaccccc ggagacacca
     1861 ccatggtgac tcaggcgatg gtggaagccg aggtgaagcg agccaagaag ggtggcggcg
     1921 tcgtcgccct gcgctagacg tggcatggaa gtgataagca gtcaggccgc aatgcgcatg
     1981 acgcagacag catagcccat agggcatcgc gtccggcgaa tcgcgcctgg cgctgaggaa
     2041 ggaggcaatc atggtagaag gttacaccag agatccgaaa cgggtggacc gggcgctgcg
     2101 ggagaagggc gtcgtggtgg tcatggggcg agaccacgcc cgaagccctc aggatgtcat
     2161 caacaccgtc cgggccatct acgaggccgg ctacatcgcc gaggtcacct tccgcatccc
     2221 cgaggaaatc ttgaaggagg ccatggccga gcttcggaag cgccgcgtcg agagcgtgaa
     2281 ggcgcgccca agcgacccca tgctcctggg cgtgggcagc gtcatcaatc cccgggaatt
     2341 ggagtccgcc atcgagatgg gtttcgacat gatcgtgggc cccgacgcgg gcatgggcgg
     2401 gagccgggag cgcatcgagt tcgtgcggat cgtccgaggg gccaaggcct tcggcgtgcc
     2461 cggaagcttc tcgccgtcgg agtttgctta cttcctggag cgcgaggacg gtctggagcc
     2521 cgatggaatc aagatcttca acgccagcat ctatgggccg agcgggattg gcgccctcct
     2581 cgccccctac cagcgggagc ggcacaacgg caagatgatc atgcccacgg gcggcgtgaa
     2641 cgtgaagacg ggggccgcct tccaggagca gatcagcaag cgtgggttct acccggtgct
     2701 gggcatgtcc gcgcccctgg aactggtgac cgagcgcaag aaggcgggcg acccggacac
     2761 gctcaaggag tcgctccaga aattcaaggc ggagttcaag ccctacaagc cagcctagcc
     2821 agcggttgtg ggaggactag attgtctagg ggccggggag ggctcggacc ttcccggccc
     2881 tcggtttttc ccggagccga cttcgcatct cgtacccaga tctgcagcgc gcacttcgcc
     2941 ccctgatcac gccggaagga gatgagcatg tcctcgagta cattgtctcc agcggagttg
     3001 agccagcgct acaaggccct gtacaccggg gccgtctcgg atatcttgga cagtctgggc
     3061 ctccgacagc aggcgctgcc gtaccccatc atgcccctga gcctggacat ggtggtggcc
     3121 ggtccggcct tcaccgggca ggggtacccc gtggaggaca tctccaacga cgattcacac
     3181 atgcggatcc ggatgctgga aagcatcaag ccgggaaccg tctcagtctg gtcgtcggcc
     3241 gggcacttcg aatcggcgca ttggggcgag attatgagca atgccgcccg tgagcgcggc
     3301 tgcaccgggg cggtggtgga cggcgggctc cgggacaccc gattcgtcct ccagatggga
     3361 ttcccggtct tctgtcgctt ccgctgctcc gccagttcta ttgggcggtg ggagatcaag
     3421 gaatggatgg tcccgattca gatcggccgc acagccattc ggccggacga tttcgttttc
     3481 ggggacgtcg acggggtggt ggtcgttccc aaggagctga cggtcgaggt cctgctcaag
     3541 accgaggaga tggtcggccg ggagaacagc atgcgggccg acctggccaa ggggatcacc
     3601 gtttccgagg tctacagaaa acacggtaaa ttctgaggca aggggccgcc gaccagggcc
     3661 tggcatccct cacccgaggc tttccctgtt taaccatcgt cgccgccctc atgctacccg
     3721 agcaatagac aagaattctt tcaccctcga ggcgtggccc cgcgtatatt ctagcgacgg
     3781 atccgaaggg agcaactttg gtgggttcat gctctctagc tcgcagggcc gtctggggca
     3841 tgggccacct gaacgctccc gcccgaacta cgaggccggc attgcccgaa accgatgagg
     3901 ccctgttcga gaaggttcag tcggggaggc aggaagcctt tgcgaccctg gtggagcgat
     3961 accagcgtag gggctatgcc gtggccctgc gccttctcgg caggggccag gatgcggagg
     4021 atgcggtcca gcaagcgttt ctcagactct atgagagccg ggcggcattt gattccaggt
     4081 gtcggctgtc cacctggttc tatcgcatcc tcaccaacac ctgcgtggat gaactccggc
     4141 gccgtcgccc cacgcttccc ctacccgaga cggaaatgcc ggcggaggat gctcccgaca
     4201 aggatctgga agcggcggag cggcaccgtc tgctccaccg ggcgctggcc gaggtgccgg
     4261 tggaggcgag gattgcgctc accctttact acggggatgg gcggtcctat ggggagatcg
     4321 gggcgatccg cgggatctcc gtgaacacgg tgaagactca tctcagacga ggtcgcctgg
     4381 cattgcgcaa ggccctgcgg gcgcaaggct tggccagttc ctgatccggg aagagacgcg
     4441 aacgactgtg gcggacgctt catggctgac aactgacggc aggcaggaag ccaagacgat
     4501 ggttaaaggc gaaagaccgg aaagaaacga aagtcaaggg acccgaagag tcgtgggaag
     4561 cgaggcctgg cttcgggcgg ccttcgcggc cccacctgaa cctccagatc ttgcgaggcg
     4621 catcatggaa cagatcgagc tgacactgga tgcggccgag ccgagtgatc ttctacagcg
     4681 gctggacctg gcggcctcct cggccggcat cactcacgtg tcgctgcggc gccgtagagg
     4741 agtctctccc gcagtcggcc cggcgggaac catcgggaac ctgctggccc aggcgcgcag
     4801 ggagctggaa gagtacttcg ccggcagacg gaccttcttc accgtcccgg tggacctctc
     4861 acacgtccct gcattcgaac gggaggccct tgaagtcgcg gctcgcatcc cttacgggga
     4921 ggtgcggagc tacaagtgga tcgccgagca attggggaag ccggatgcgg cgcgcgccgt
     4981 ggggggcgcc atggccggga atcctgtccc gatcatcgtg ccctgccacc gcgtggtgaa
     5041 gaccgatggg ggactaggtg gatactcctt cggcctgatg cggaaggagg ccctcctgaa
     5101 cctggagagg agcactaccc cctacatcgg ctgcctcaca accaagttct tctgccggcg
     5161 aggctgtcat gccgagcaac gcatcggcga aggcaaccgt atcaagttct tctcgccggc
     5221 cgacgcgcag gccagcggct accgcccgtg cagcgtgtgc cagcccgctt gacgccccct
     5281 ggtgacggca ggggcgggcg cggcatctct tctcccgcat tcgcagggtt ctgcccagtg
     5341 cgccaccgag gagaatgggt cgctctcctc aggcccctgc atacagagtc atggtgttca
     5401 cgggtagacc caagagcttc aggtagaaga acaactgata cttgtggttc acccagtggt
     5461 cggccatgcc caggctcacc cgccacagcg gcatgggcgg cccccacgga accgacacca
     5521 tcttggcctg gaagtcacca tcgctcacgg acaggagcgc ggctttggat tcgtcccacc
     5581 cgcgtgaagc ctcccgagcc gcaatctccg gggtctttgt gttcttcaag tcttcttcca
     5641 aaaacttctg gaaggcttcg ggggacggga aggcattgtt cacggccgcc acgaagaccc
     5701 ccggacacgt ggacaggtga tgcaggagct gacccaagct catgtaatcc cctttcgccg
     5761 gtttccagtc cagcttgttg gttggggcga gtgaaaccag cttggccgtg ggtcggtaga
     5821 tggattcggc gtactcaatg aattgtttgg gtgtcatgtc tcccatcctc cttgttgtcg
     5881 aatccggaat cgagttggat gagcatcccg atggctggca atctttgggg aagccaaacg
     5941 aggatacagg gtggttgcgg gggatcggtc tctacagggg cgtgatgccc cggtaagacc
     6001 agtccaggac ctcgacggtg tggacggcgc gcatgggcag gccgcgcggg cgaagaccca
     6061 tggcgatttg aagcaagcat ccaggattcc cggcggcgac aacctgggcg ccggtctcgg
     6121 ccagcctgtc cagcttccgc tccagaaact cccgggacaa ttcagggtgg agcaggttat
     6181 agacgccggc gctcccgcag caaaagtcgg cgtcgcgaag cggaaccaac tccaggccgg
     6241 ggatcgccgc gagcagcgtt cgcggctcta cccgcacccg ctggccatgg gccaggtggc
     6301 aggcgtcgtg atacgccacc cgaagcttca ccgggtgaag cgtacccacg ggtcccgcct
     6361 ccgccagaat ctgcgagacg tcgcggaccc gttggctgaa ggcggcggcc cggccggcat
     6421 acctggggtc gtccttgagc aagtcgccat agtccttcat gacggatccg cagccggctg
     6481 cgttgatcgc taccaggtcg gccccggcag cctcgaaaac gtcgatggcc ttccgggcca
     6541 gggccttgcc cttttctcgc tcgccctcat ggaccaagag cgacccgcaa cagccttgct
     6601 tccgcggcgc caccacctcg aatccgttgg ccgccaggac ccgcgcggtg gccagattca
     6661 catccgggaa gaaggcgtgc tgcgcacatc caaggagcag ggcaacccga ccacgacgcg
     6721 ttccgacggc cggggtcacc tcgggaaagt gggtcaccag gccggggtcc ggcaacgccg
     6781 gaagcaatcc ctccatggcg ccccagcgac ccagcagctt caagaggcct gtgctccgca
     6841 gcagatgctg gatgcccagc ttctgataga gtcgcatggg cgccaggagg atccgcaacc
     6901 gggagggatc ggtgaaggtg tcgaggatga actttcggaa aaggcgatcc gagaaggagt
     6961 gggtgtactt ccggaccacc tgtcctcggg ccgcctccat caggtgtccg aagtccacac
     7021 cggacggaca cgccgtctcg cacgcccggc acacgaggca gagactcatg tgtttctgga
     7081 agctgtcgcc gatctcgaca cgcccttcgc ccgcggcccg catgaggtag atgcgacccc
     7141 gcggggagtc catctcgttg cccagaacca ggtaggtggg acaggtgggc aggcagaacc
     7201 cgcagtggac gcagtccaga attttttcca tctccggagg atcgagagga tcgaaggccc
     7261 cgagcgccct cggtgccgga gtctctttca ctgcctcagc tacctgtgga ctgcttgcca
     7321 tggaaactcc gcctcgcgtg cctgcgcact gaataaatct gcctcgggaa ggttgccaga
     7381 tcagagtcct ccgacgaatc ggccggggtt caggacgcgg cccgggtcga actggtcttt
     7441 gagccctcgc atctgagcca aggtgtctcc gaccggaccc cagacatcca cggccgcctt
     7501 cacctctggt ggcgcctgca gcacgaccag gctgcccttc gcctcgatgg cgaaggcgcg
     7561 aagcggggcc accgtcccgg tcaggcgcac gatggaatcg ctggcggcac tgtcagcctg
     7621 gaggtagtag cgcacgatcc ccgtgcccgc ctccgagatt gtggacacat gcagcccctg
     7681 tttggccgcg acctcctccc cttgtcgcat ggcctcggcc acctcgctga tgaggacgcc
     7741 ggccttgagg accattgccg gcccaccgtt ccccggagcg aaatcgcgca cggcgcccca
     7801 gaactgctcg tgttcctcgc cttccagcat caggccgtcg tccccgccgg cctggcgacc
     7861 catccccctc gcctgttcga gttg
//
LOCUS       DBNGZC010000617         2062 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2109075, whole genome shotgun sequence.
ACCESSION   DBNGZC010000617 DBNGZC010000000
VERSION     DBNGZC010000617.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2109075"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1559)
                     /locus_tag="AC16G0_10715"
     CDS             complement(<1..1559)
                     /locus_tag="AC16G0_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5100120.1"
                     /translation="MGEAGGKQEGITVDGLARMLRDGKITRRGFLVRAAGLVGGLAAA
                     EGLLARVAGAQTTRKTQVVIAQGGDLSKLDPHLSTSGWDLAITLNVFDNLTSRHPDGK
                     IYPGLATEWKLVNPTLWQFKLRSGVKFHNGDPFTSADAKFSIERSYDPNMRVSMVRTN
                     FQTIDHIEAPDAHTANFYTKKPDPLLPARFSFSGGQIMPKKYLEAVGAEAFNLKAVGT
                     GPVKLAEWVKDDRVVLDANPDYWQGKIDADRAVFRPIPESASRIAALLAGEVDLITKL
                     QPDHADRVAKHPTTKVEGVLYAGLYVLMANSRRPPLDNPKVKQALSLAIDREAIVKEL
                     FHGQGIIPSQPIAKGDNHFDESLPPLKYDPALAKQRLKEAGYKGEEVVIESGQGLIVN
                     DKEMGEAVVSMWREVGVNAKLEILETTVRAQKLREKTFKGFFWADPTSILGDPDGMMW
                     RILAPGGIYEFWRHPRFDELGDAARFSVDEKFRGQAYKEMTQIFHEQVPWIGVIQPIE
                     LYGLQKYMDWKA"
     gene            complement(1809..>2062)
                     /locus_tag="AC16G0_10720"
     CDS             complement(1809..>2062)
                     /locus_tag="AC16G0_10720"
                     /inference="COORDINATES: protein motif:HMM:NF037832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HIR5100121.1"
                     /translation="LRGATDGAIWEAARREVYVIVSKDNDFRQLSFLHGAPPKVIWLS
                     VGNAGTEAILRFLRSQRAEIQAFGDDTETSLLVLTLAPS"
BASE COUNT          325 a          678 c          647 g          412 t
ORIGIN      
        1 gccttccagt ccatatactt ttggagaccg tacagttcga ttggctggat caccccgatc
       61 cagggcactt gctcgtggaa gatctgggtc atctccttgt aggcttgtcc ccggaacttt
      121 tcatccacgc tgaagcgggc ggcgtcgccc agctcgtcga agcgcggatg gcgccagaac
      181 tcatagatcc cgcccggggc aaggatgcgc cacatcatgc cgtcgggatc ccccaagatc
      241 gaggtggggt cggcccagaa gaaacccttg aacgtctttt cccggagttt ctgggcacgc
      301 accgtggtct ccaggatctc gagcttggcg ttgaccccga cctcccgcca catggacacg
      361 acggcctcgc ccatctcctt gtcgttcacg atgaggccct ggccgctctc gatgacaacc
      421 tcctccccct tgtaccccgc ctccttgagg cgctgcttgg ccagggcggg gtcatacttc
      481 aacgggggca ggctctcatc gaagtggttg tcccccttgg cgatgggttg gctcgggatg
      541 attccctggc cgtggaacaa ctccttgacg atcgcctccc ggtcgatggc gagcgacagg
      601 gcctgcttga ccttcgggtt atccagcgga ggacgtctgg agttcgccat caggacgtag
      661 agccccgcgt agagaacccc ctccaccttg gtggtgggat gcttcgccac ccggtcggcg
      721 tggtcgggct ggagcttggt gatgaggtcc acttcgcccg cgaggagcgc cgcgatgcgg
      781 gaggcgcttt cggggatcgg ccggaacaca gcccgatccg cgtcgatctt cccctgccag
      841 tagtccgggt tggcgtccag caccacgcga tcgtccttga cccactcggc cagcttgacc
      901 ggccccgtcc ctaccgcctt caagttgaag gcctcggccc cgacagcctc caggtacttc
      961 ttgggcatga tctgcccgcc ggagaagctg aagcgggccg gcaacagcgg gtccggcttc
     1021 ttggtgtaga aattcgccgt gtgggcgtca ggcgcctcga tgtggtcgat ggtctggaaa
     1081 ttcgtcctca ccatcgagac ccgcatgttg ggatcgtagg accgctcgat gctgaacttc
     1141 gcgtcggccg acgtgaacgg atcaccgttg tggaacttga cccccgaacg cagcttgaac
     1201 tgccacaggg tcggattcac gagcttccac tcggtggcca ggcctgggta gatcttgccg
     1261 tcgggatgcc ggctggtgag gttgtcgaac acgttgagcg tgatggcgag gtcccagcca
     1321 ctcgtgctga ggtgcgggtc gagcttggag aggtctccgc cctgggcgat gaccacctgg
     1381 gtcttccgcg tcgtctgcgc cccggccacc cgggcgagaa gcccctcggc tgcagccagg
     1441 ccgcccacca gaccggccgc gcggaccagg aagcctcgcc gggtgatctt cccgtcccgg
     1501 agcatcctgg ccagtccgtc caccgtgatg ccttcctgct tcccccctgc ctctcccatg
     1561 gccctgctcc tttcgccggt gatcgagaca cactttgctt tcgagcctgc cgcggatccg
     1621 gccctccccg cccttatcgc ggcccggcgt ccgccccgtt acaaactctc cgccggtggc
     1681 gccgagcatc ccgcgccccc ggtgcagttg tcaaggcgga attttgggtg gattgggtcc
     1741 tgaggtgagg tggaggtggc gggctggggt tggttaatga ccaatgctcg agcgccagtc
     1801 ttcaggacct aactgggagc gagggtaaga acgaggagcg aagtctccgt gtcatcccca
     1861 aaggcctgga tctctgcgcg ctgacttcgc aggaagcgaa ggatggcctc cgtcccggca
     1921 ttgcctaccg atagccagat gaccttgggc ggagcgccgt ggagaaaact gagttgtcga
     1981 aagtcgttgt ctttggagac aatcacgtag acctctcgcc gggccgcttc ccagatagcc
     2041 ccgtctgtcg cgccccgcag gc
//
LOCUS       DBNGZC010000618         2197 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_736326, whole genome shotgun sequence.
ACCESSION   DBNGZC010000618 DBNGZC010000000
VERSION     DBNGZC010000618.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_736326"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..1178
                     /gene="pdxS"
                     /locus_tag="AC16G0_10725"
     CDS             297..1178
                     /gene="pdxS"
                     /locus_tag="AC16G0_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal 5'-phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HIR5100122.1"
                     /translation="MEKGTWTVKVGLAQMLKGGVIMDVVTPDQARIAEDAGACAVMAL
                     ERVPADIRAAGGVARMADPTVIEAIKRTVTIPVMAKCRIGHFVEAQVLEALGVDYIDE
                     SEVLTPADEEHHIFKHPFTVPFVCGCRDLGEALRRIGEGAAMIRTKGEAGPGNVVEAV
                     RHARAVLGAIRRLQGLTQDELMAEAKNLRAPYELVAEVAQTGRLPVVNFAAGGIATPA
                     DAALMMQLGVDGVFVGSGIFKSADPAARAKAIVQATTHFRDAKLLGEVSRGLGEAMPG
                     LDVRSMKPEDLLATRGW"
     gene            1273..1848
                     /gene="pdxT"
                     /locus_tag="AC16G0_10730"
     CDS             1273..1848
                     /gene="pdxT"
                     /locus_tag="AC16G0_10730"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006551387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal 5'-phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HIR5100123.1"
                     /translation="MPSVGVLALQGDFREHREMLERMGASSAEVRLPRDLPGLDGLII
                     PGGESTTIVRLMRTAGLLSPLKGLAADGFPMWGTCAGMILLARRLDTTGVPALEVMDI
                     AVRRNAFGRQVDSFEADLPIAALGDSPFHAVFIRAPIIEEVGPGVEVLARLSDGTPVA
                     ARQGRLLATAFHPELTADDRLHRLFLESVKR"
     gene            complement(1918..>2197)
                     /gene="tatC"
                     /locus_tag="AC16G0_10735"
     CDS             complement(1918..>2197)
                     /gene="tatC"
                     /locus_tag="AC16G0_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033281 - TAT protein transport complex
                     [Evidence IEA];
                     GO_function: GO:0009977 - proton motive force dependent
                     protein transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR5100124.1"
                     /translation="VDFTLKFLLAFGVIFELPLAMVIAARMGMVTPQFLSRNRKYAIL
                     VNFVIAAILTPTPDIFNQSLMALPMCVLYEVGILASRFVAPRPKPAEV"
BASE COUNT          416 a          669 c          718 g          394 t
ORIGIN      
        1 gagagaagca ctcggtatgc caatccgcaa tgaataatgg ccaggcctcg aactgcgcgg
       61 aattccgagc gagtatacga tttcatgacc tacggcgggg tatcgtccgg cactccgtgg
      121 aggatgaaag tggaaaagtt acatgatcga gaatgtaaaa cttgctggca tcgccgtccc
      181 ttattcccct cccgcaacgc tcccggaaga ctccgcgcca cgcctcgcgg gaacgcgttg
      241 acaccttagg acatagacga ctacaatcca atccgattca aaaagggagg atgatcatgg
      301 aaaagggaac ttggacggtg aaggtgggac tggcccagat gttgaagggc ggcgtcatca
      361 tggatgtggt cactccggat caggcccgca ttgccgagga tgccggcgcc tgcgccgtga
      421 tggcattgga gcgggtcccg gctgatatcc gcgcagccgg tggcgtcgcc cggatggccg
      481 atcctacggt gatcgaggct atcaagcgga ccgtcaccat cccggtgatg gccaagtgcc
      541 ggatcggcca ctttgtggag gcccaggtcc tggaggctct gggagtggat tacatcgacg
      601 aatccgaggt cctgacgccg gccgatgagg agcaccacat cttcaagcac cccttcacgg
      661 ttcccttcgt ctgcggctgt cgcgatctgg gcgaggcgct ccggcggatc ggcgaagggg
      721 cagccatgat ccggaccaaa ggcgaggctg gacccggcaa cgtggtcgag gcagtccggc
      781 acgcccgggc cgtcctggga gccatccgtc ggctccaggg tctcacacag gacgagttga
      841 tggcggaagc caagaatctg cgcgccccct atgagctggt ggcggaagtc gcgcagaccg
      901 ggcgtctccc ggtcgtcaac ttcgcggccg gggggatcgc cacccctgca gatgcggccc
      961 tgatgatgca gctcggtgtg gatggggtct tcgtgggttc ggggatcttc aagtccgccg
     1021 accccgcggc ccgggccaag gccatcgtcc aggcgacaac ccacttcagg gatgccaagc
     1081 tcctgggcga ggtctcccgc ggtctaggtg aggccatgcc tggcctggac gtccgcagca
     1141 tgaagccgga ggatctgctg gcaacccgcg gctggtaacg gaacaaatcc catttggcca
     1201 cttctccatg acgctcgggt tccgcgttca cggagatttg gtctatggct catcggacat
     1261 tcaccactcg ccatgccatc cgtcggcgtc ctcgccttgc agggagactt tcgtgagcac
     1321 cgggaaatgc tggagcgcat gggagcgtcc agcgccgagg tgcgcctgcc gcgagacctt
     1381 ccgggtctcg atggtctgat catcccgggg ggcgagagca ccaccatcgt ccgcctcatg
     1441 cggactgccg gcctgctctc accgctcaag gggctggctg ccgacggctt ccccatgtgg
     1501 ggtacgtgtg ccggcatgat cctcctggcc agaaggctgg acaccaccgg ggtccctgcc
     1561 ctggaggtca tggacatcgc cgtgcggcga aacgctttcg gccgccaggt ggacagcttc
     1621 gaggccgacc tccccatcgc tgcccttggg gactccccgt ttcacgcagt cttcatccgc
     1681 gccccgatca tcgaggaggt tggtcccgga gttgaggtgc tggcgcggtt gagtgacggc
     1741 actcccgtag ccgctcgcca gggccgcctc ctcgccacag ccttccatcc cgaactcaca
     1801 gcagacgatc gcctacaccg gctcttcctc gaatctgtga agaggtagac ccggtccgcg
     1861 gtgacggcac gatccctggc taaagtagtg gagtgccaga gggtcgaagc ggaattgtta
     1921 gacttcagcg ggcttggggc ggggggcgac gaatcgtgag gcgaggattc ccacctcgta
     1981 gagcacgcac atgggcagtg ccatcaaact ctgattaaag atatccgggg ttggggtgag
     2041 aatcgccgcg atgacgaaat tgaccaggat ggcgtacttc cggttccgcg acaggaattg
     2101 cggcgtgacc atgcccatgc gggcggcaat gaccatcgcc agggggagtt cgaagatcac
     2161 gccgaaggcg agcaggaact tcagggtgaa atctaca
//
LOCUS       DBNGZC010000619         1961 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_541327, whole genome shotgun sequence.
ACCESSION   DBNGZC010000619 DBNGZC010000000
VERSION     DBNGZC010000619.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_541327"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /gene="pyrF"
                     /locus_tag="AC16G0_10740"
     CDS             complement(<1..361)
                     /gene="pyrF"
                     /locus_tag="AC16G0_10740"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009470329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR5100125.1"
                     /translation="MAHPNSRLIVALDVPTLGEASDLARRLAPYVAAVKIGSQLFTAE
                     GPRAVAAMRDLDLRVFLDLKFHDIPNTVAGAVAAACRLGVWMLNVHSSGGPTMLQAAA
                     RAASAAVDGGASKTPTPL"
     gene            620..1804
                     /locus_tag="AC16G0_10745"
     CDS             620..1804
                     /locus_tag="AC16G0_10745"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5100126.1"
                     /translation="MRVVGIALCVAICLFLFAPSAAAFHDAPAVREGMLRATDLLLNR
                     DADLAEAECQRLILLPQGEAPGRFCLGLVTLTRAEDVDDPTPDLDRFLSQAAGAIAAG
                     ESQERSQPADAEVKLLLGLAQGTKALVDGTRKNYLAAFQALREAQRRFQEALQLDPNL
                     IDAHYGIGLYQYALGHLPVLLKPLVSIVLPPGDPAHGVEELTRVAEHGTYLRMTARVA
                     LLQLFVGEERRYAEALRLGRDLLQRYPGNPDLYFATAFAASELGRFPEALGVARRVAR
                     NMAESRPRFGPDLAARYNQLMGKVYMDQGEYATALTFFQRAIQAPTPPRNAWVTAWAW
                     TRSGMIHDLQGEREEAVRRYRQALAVGKEGPARSLAQRYLETPYRGESRPPSGDGGGA
                     SP"
BASE COUNT          322 a          643 c          686 g          310 t
ORIGIN      
        1 ccagcggcgt tggcgtcttg gaggcgccgc cgtcgactgc cgctgacgcg gcccgagcgg
       61 ccgcctgcag catggtcggc ccgccgctac tgtgtacgtt caacatccag accccgaggc
      121 gacaggccgc cgcgaccgcc cccgccacgg tgttggggat gtcatggaac ttgagatcca
      181 ggaagacgcg gagatccagg tcgcgcatgg cggccacggc ccgagggccc tcggcggtga
      241 agagctggct cccaatcttc acggcagcca catacggcgc gagacgccgg gcgagatccg
      301 aggcctcgcc aagcgtgggc acgtccaggg cgacgatcag gcggctgttc gggtgagcca
      361 tcgtgctctc cgaaggattg acgcggctct ctggcaacca ggcattccgg ttcagtggat
      421 cgcccggctg tccgcttgcc cgagcgccag cagggtatca cggaggcgga gcgagcgtca
      481 atcaacgggg ggaaggaagc caggcgctgt tggctcggag cagtgggaca gagggttgaa
      541 gctgatggct gacggccaag ggctgagggt tgtctcccgg acgttgacac cggaccgctg
      601 gtcggcttag agtgagccta tgcgtgtcgt cgggatcgcc ctgtgtgtcg ccatctgtct
      661 tttccttttc gccccgagtg ccgccgcctt ccacgatgca ccggccgttc gtgagggcat
      721 gcttcgcgcg accgacctgc tcttgaaccg ggatgcagac ctggccgagg cggagtgcca
      781 gcgcctcatc ctgcttcctc agggcgaggc gcccggtcga ttctgtctgg gtctcgtcac
      841 gctgacccgg gcagaggacg tggacgatcc gacgccggac ctggatcgtt ttctctcgca
      901 agcggccggg gccatcgccg ccggcgaatc ccaggagcgg tcccagcctg cggatgcgga
      961 ggtgaagctc ctcctgggcc tcgcccaggg gaccaaggcg ctggtggacg gcacgcggaa
     1021 gaattacctg gctgccttcc aggccttgcg ggaggctcaa cgccggttcc aggaggcgct
     1081 ccagctcgac cccaacttga ttgatgccca ttacgggatc ggcctctacc agtacgccct
     1141 gggacacctg cccgtgctgc tcaaaccgct ggtctccatc gtgctgcccc caggcgatcc
     1201 agcgcacggc gtggaagagc tcacgcgagt ggcggagcat gggacgtacc tcagaatgac
     1261 ggcgcgggtg gcgctgctcc aactcttcgt gggggaggag aggcgatatg ccgaggcgct
     1321 gcgactgggg cgcgatctcc tccagcggta tcccgggaat ccggatctct acttcgcgac
     1381 ggcctttgcg gcctcggaac tgggccgctt ccccgaggcc ctgggggtcg ctcgacgcgt
     1441 ggcccggaac atggccgagt cccgcccgcg attcgggccg gacctcgccg cacgctacaa
     1501 tcagctcatg ggcaaggtct acatggacca gggcgagtat gccacggccc tgaccttttt
     1561 ccagcgggcc atccaggcgc cgaccccgcc acgcaatgcg tgggtcaccg cctgggcctg
     1621 gacccgctcg gggatgatcc acgatctcca aggggagcgg gaggaggccg tgcgccggta
     1681 ccgtcaggcg ctggccgtgg gaaaggaagg gccggctcgc agcctggcgc agcgttacct
     1741 ggagacgccg tatcgcgggg agagccgtcc cccctcggga gacggaggag gggcctcgcc
     1801 ctgacgaagg gccaccgctg tgggaggatg atgaagaccc cgccttccgt ccaggctact
     1861 caagaacgtc cagatgcacg gcggcgcacc caccggaggc gggcacccat ccaaaggatg
     1921 ggtacccggc agacggatat attgcagcag catggcagga a
//
LOCUS       DBNGZC010000620         2013 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_639776, whole genome shotgun sequence.
ACCESSION   DBNGZC010000620 DBNGZC010000000
VERSION     DBNGZC010000620.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_639776"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="AC16G0_10750"
     CDS             complement(<1..633)
                     /locus_tag="AC16G0_10750"
                     /inference="COORDINATES: protein motif:HMM:NF013921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR5100127.1"
                     /translation="MDAPGTLHHVMVRGIERTVLFRADTDREDFLGRVAALAEGGAFT
                     IYAWALLPNHAHLLVQTGQRPLARSMRSLLTGYAGAFNRRHRRVGHLFQNRYKSIVVE
                     EAAYLLELTRYLHLNPVRAKVLPDLRALDRYPWTGHSALLGTVPRPWQATQTVLAQFG
                     ATRRRALHAYRQFVAAGRPQGRRPELQGGGLVRSLGGWQAVTALRRGREAF"
     gene            complement(973..1296)
                     /locus_tag="AC16G0_10755"
     CDS             complement(973..1296)
                     /locus_tag="AC16G0_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5100128.1"
                     /translation="MKGQKLDVVHGSGNVFRDLGHSNADVAQFKAILATEIIKVLDRE
                     GLSVRAAQARTGITAADFSRIRNADLGRFTVDRLMSIINRLGSRVEVKVKIRRAALIE
                     GRVPV"
     gene            complement(1293..1640)
                     /locus_tag="AC16G0_10760"
     CDS             complement(1293..1640)
                     /locus_tag="AC16G0_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR5100129.1"
                     /translation="MTRRTRPVSWVKAALKAFAAFPAEAQSICLTALTIAAEGGKADL
                     AKPLHGLGTGVFEIALVFRGAAFRVVYAVQLGAPIWVVHAFRKKSTLGIKTSQRDIEL
                     IKDRLRQLKEIVE"
BASE COUNT          362 a          663 c          659 g          329 t
ORIGIN      
        1 gaaggcctcc cggccccggc ggagggcggt gaccgcctgc cagcctccga gactccgcac
       61 gagccccccg ccctggagct ccgggcggcg gccctggggc cggccggccg ccacaaattg
      121 acggtaggcg tgaagggccc gacgtcgggt ggcgccgaac tgggcgagga cagtctgggt
      181 cgcctgccag gggcggggga cggtccccag gagggcgcta tggccggtcc acgggtagcg
      241 atccagggcg cggagatcgg ggagaacctt cgcccggacc gggttgaggt ggaggtagcg
      301 ggttagctct aagaggtagg cggcctcttc taccacgatg gacttgtacc ggttctgaaa
      361 gaggtggccg acccggcgat ggcggcggtt gaaggcgccg gcataccccg tgaggaggct
      421 tcgcatgctc cgggcgaggg gccgctgccc agtctggacg aggaggtggg cgtggttcgg
      481 gagcaacgcc caggcgtaaa tcgtgaaagc cccgccctcc gccaaggcgg ccacccggcc
      541 gaggaagtct tcccggtccg tgtccgcccg gaagaggaca gtccgttcga tccctcgcac
      601 catcacgtgg tggagcgtgc cgggcgcatc caagcgagcc tgacggggca tagcgaaacg
      661 atcctagcac aacatgctat acatggcaac gtccagtatc cgtcccgtat cgcgccacat
      721 ggcaaggcgt cccggattat ggattacgcc ccaacgccgc caggacgccc atcagccgac
      781 cgaggtgctc ggtccaaatg taggcgagca acggccaggc ctccgctgcg cggcgagccc
      841 gaacccagga ttatctaggg tgcgatctcg atccttgccc ggggctagcc gggtcggagc
      901 gaggggccgg cgctgtcggg tgggtcgggc cagcacctga cggtccgcac tcctcggaaa
      961 gcggcccgta tctcagaccg gcaccctgcc ctcgatcagt gccgctcgcc ggatcttgac
     1021 ctttacttcc actcgcgacc cgagccgatt gatgatggac atgagccgat cgaccgtaaa
     1081 ccgtccgagg tccgcattcc ggatgcgcga gaagtcagcc gccgtgatcc ccgtccgggc
     1141 ctgcgccgcg cgcacgctca ggccctcccg atcgagcacc ttgatgatct cggtcgccag
     1201 gatggccttg aactgtgcga cgtccgcgtt cgagtgcccg aggtcgcgga acacattccc
     1261 gctgccatgc accacgtcca gcttctggcc tttcattcca cgatctcctt cagttgccgc
     1321 aagcgatcct tgatcaactc gatgtcgcgc tgggacgtct tgatgcccag cgtcgatttc
     1381 ttccggaagg catgcacgac ccaaatcggc gcgccgagct gcaccgcgta caccacacgg
     1441 aacgcggcac cgcgaaacac cagcgcaatc tcgaacacgc ccgtccccag gccgtgcagc
     1501 ggcttggcca gatctgcctt gccaccttcg gccgcgatcg ttaaggcggt cagacagatc
     1561 gactgggctt ccgccgggaa cgcggcaaag gccttgaggg cggccttgac ccaggacacg
     1621 ggccgggtgc gtctggtcat gggctgtcgc tcaatccccg agtgcgaatc cgcaccttct
     1681 cgcgccgccg cttcgatgcc catatgttgt catatgtgac aacacctgtc aagccaggtc
     1741 atgcgtaaag tggaattccg gggacaccat acttatctat gcggtccgga agtaaggctc
     1801 catatccgcc tggaccgcat agaacaagtc aaagagaaag gtgaccgcgc ccgaaggggg
     1861 tgggcagacg acgtaatgcg ttgttgacga agtggactta ttgcgcttcc gccctgtcga
     1921 ccgctttcgc gatccccgag gtcgagacgc ccaggtgcct ggtcgcctcg gccaaggaga
     1981 gcccgagtcg cgtcacgaga tggacagcga gtg
//
LOCUS       DBNGZC010000621         2446 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1617192, whole genome shotgun sequence.
ACCESSION   DBNGZC010000621 DBNGZC010000000
VERSION     DBNGZC010000621.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1617192"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="AC16G0_10765"
     CDS             <1..185
                     /locus_tag="AC16G0_10765"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR5100130.1"
                     /translation="TGVWETANWHEREVYDLFGVSFDGHPDLRRILMPDDWEGHPLRK
                     DYPVEASPKWWEEEPA"
     gene            203..1357
                     /gene="nuoD"
                     /locus_tag="AC16G0_10770"
     CDS             203..1357
                     /gene="nuoD"
                     /locus_tag="AC16G0_10770"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HIR5100131.1"
                     /translation="MTLNMGPQHPSTHGVLRLVLELDGEIVVRCVPHIGYLHTGIEKG
                     LESKRYQQGIPLTDRLDYLAPLSNNLVYVLAIEKALGVSVPERSQTIRVLLTELTRIK
                     SHLVWLGTHALDVGAMSVFLYCFREREQILDMYEAVSGQRMMSTYFRVGGLAADIPPG
                     FEQQVQGFLTTFPDRLAEYEALLTQNPLWRQRTVGIGTIKAADAVALGLSGPSLRASG
                     VAYDVRRAHPYSGYESYQFDVPIGKAGDVFDRYVVRVAEMRQSHRIALQALQRLSPGP
                     INVADPKLIPPPKPLLKASMEALIHHFKLYSEGFSVPAGEVYQAIEAPKGELGVYLVS
                     DGSPRPYRVHYRAPSFVNLQALERMVEGRLVADVVAVIGSLDIVLGEIDR"
     gene            1524..2024
                     /gene="nuoE"
                     /locus_tag="AC16G0_10775"
     CDS             1524..2024
                     /gene="nuoE"
                     /locus_tag="AC16G0_10775"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HIR5100132.1"
                     /translation="MDTQVISEATRKRFDELVKRYPEKRSVLIPMLHEVQAEVGYLSP
                     EAIEWVAGYLGLSPADVMSVASFYDMLHLEPVGRHMIYVCHNLSCALLGAERLIRHLE
                     SRLSTRMGETTPDGRITLKRMECLAACGMGPAIQVDGVFHEHMTPEKLDALLEALQKD
                     AGAPRG"
     gene            2058..>2446
                     /locus_tag="AC16G0_10780"
     CDS             2058..>2446
                     /locus_tag="AC16G0_10780"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052520.1"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HIR5100133.1"
                     /translation="MGVVFERILTQYKEIPNYRGTIQEYLAHGGYQALPKVLKDLQPA
                     DLIDLVKRSGLRGRGGAGFPCGVKWGFIPKDSPKPKYLVVNSDESEPGTFKDRMLIEE
                     EPHQLIEGIALASYAIGCHSAFIYCRG"
BASE COUNT          426 a          791 c          837 g          392 t
ORIGIN      
        1 tgaccggcgt gtgggagacc gccaactggc acgagcgcga ggtctacgat ctgttcgggg
       61 tctccttcga tgggcacccg gaccttcggc ggatcctgat gcccgacgac tgggaggggc
      121 atccgctgcg caaggactac ccggtggagg cctcgcccaa gtggtgggag gaggagccgg
      181 cgtgaccgcc ccgtcccata ccatgacctt gaacatggga ccccagcacc cctcgacgca
      241 cggggtgctc cgcctggtgc tggagctgga cggggagatc gtggtccgct gcgtccccca
      301 tatcggctat ctgcacaccg gtatcgagaa ggggctggag agcaagcgct accagcaggg
      361 catccccctc accgaccgtc tcgactacct cgccccgctg tccaacaacc tggtctacgt
      421 cctggccatc gagaaggccc tgggcgtcag cgtgcccgag cggtcgcaga cgatccgggt
      481 cctgctcaca gagctgacac ggatcaagag ccacctggtc tggctgggca cccacgccct
      541 ggacgtgggc gccatgtcag tcttcctcta ctgcttccgc gaacgcgagc agatcctgga
      601 catgtacgag gcggtgagcg gccagcggat gatgtccacg tacttccgcg tgggcggcct
      661 ggcggcggac atccctcccg gcttcgagca gcaggtgcag ggcttcctga ccaccttccc
      721 ggaccggctc gcggagtacg aggcgctcct cacccagaat cccctgtggc gccagcgcac
      781 cgtggggatc ggaacgatca aggcggcgga tgccgtcgcg ctcggcctga gcgggccgag
      841 tctgcgggcc agcggcgtgg cctacgacgt tcgccgggcc cacccgtaca gcggttacga
      901 gagctaccag tttgacgtgc caatcgggaa ggccggcgat gtctttgatc ggtacgtggt
      961 ccgggtggcg gagatgcggc aaagtcaccg catcgccctg caggcgctgc agcggctctc
     1021 gccaggaccg atcaacgtcg ccgatcccaa gctgattccc ccgcccaagc cgctgctcaa
     1081 ggcatccatg gaggcgctga tccaccactt caagctgtac tcggagggct tcagcgttcc
     1141 ggcaggggag gtgtatcagg ccatcgaggc gcccaaggga gagctgggcg tctacctggt
     1201 gagcgacggc tctccccggc cgtaccgggt ccactaccgg gccccctcct tcgtcaacct
     1261 ccaggcactg gagcggatgg tcgagggacg gctcgtggcc gatgtcgtcg ccgtgatcgg
     1321 ctcgctggac atcgtgctgg gggagatcga ccgctgagaa ggcaactgcg caagcgttcg
     1381 gcaatcagca gtcagcgatc aggttgagat gcccgacggg agtgtttggc atgtgtcggg
     1441 ccatccgctg attctgaggc tgagggctga tagctgaacg ctgcaatggc ttgtgcactc
     1501 gactcgcgca gacgggcgag gccatggata cgcaagtgat ttccgaggca acgaggaagc
     1561 ggttcgacga actggtcaag cgctaccccg agaagcgctc ggtgctgatc cccatgctcc
     1621 acgaggtcca ggcggaggtg ggctatctct cccccgaggc catcgagtgg gtcgccggct
     1681 acctggggct cagcccggcc gacgtgatga gcgtggccag cttctacgac atgctccacc
     1741 tggagccggt ggggcggcac atgatctacg tgtgccacaa cctgagctgc gccctcctgg
     1801 gcgccgagcg gttgatccgc cacctggaga gtcgcctgag cacccggatg ggcgagacga
     1861 cgccggacgg gcgcatcacc ttgaagcgga tggagtgtct ggccgcgtgc ggcatgggcc
     1921 cggcgatcca ggtagacgga gtctttcacg agcacatgac gccggagaag ctggacgcct
     1981 tgctggaagc attgcagaag gacgcagggg caccccgcgg gtagcgggcc gcccggtcgc
     2041 gggagacggg ggcgaaggtg ggcgtggtct tcgagcggat cctgacccag tacaaggaga
     2101 tcccgaacta ccgcgggacg atccaggagt acctcgccca cggcggctac caggcgctcc
     2161 ccaaggtcct gaaggacctg cagcccgccg acctgatcga cctggtgaag cggtccggcc
     2221 tgcgcgggcg gggtggtgcc ggcttcccct gcggggtgaa gtggggcttc attcccaagg
     2281 actcccccaa gccgaagtat ctcgtggtca actccgacga gagcgagccc gggacgttca
     2341 aggaccgcat gctcatcgag gaggagccgc accagctcat cgagggaatc gccctggcca
     2401 gctacgccat cggctgccac agcgcattca tatactgccg gggcga
//
LOCUS       DBNGZC010000622         3668 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1125330, whole genome shotgun sequence.
ACCESSION   DBNGZC010000622 DBNGZC010000000
VERSION     DBNGZC010000622.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1125330"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="AC16G0_10785"
     CDS             <1..1156
                     /locus_tag="AC16G0_10785"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5100134.1"
                     /translation="RRIPQVVLDAAPGSPEASDLEAAARSGRGPGAELPKLGPEDVAA
                     CVYTSGTTGRPKGALLTHRNLTANVESFHEVLKLAEGEVFLAVLPLFHAYGGTVLLLE
                     PLSVGATIVLEPRFVPDLVLRALAEHRVSFFAAVPSMFAVLAKHPRPAADFSAWRLCI
                     SGGAPLPPPVLEAFEAKYGVPIYEGYGPTECAPVLTVNPPGGTRKVGSVGPPIPRVEL
                     RIVDERGQPVSRGEVGEIVARGPNVMRGYLNRPEETDEVLQDGWYHTGDLGRVDQDGY
                     YYIVDRKKDLILVGGLNVYPREVELVLADHPAVAEAAVIGIPDPVRGEVARALVILKD
                     GQQVGVHALLQWCRQRLANYKVPRTITIVPELPRTATGKVLKAALRGRGE"
     gene            1343..1951
                     /locus_tag="AC16G0_10790"
     CDS             1343..1951
                     /locus_tag="AC16G0_10790"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR5100135.1"
                     /translation="MVERQLKGRDITDPRVLWAMGKVRRHLFVGPGLRGEAYTDRALP
                     IAEGQTISQPYIVALMTQLLELKGSERVLEVGTGSGYQAAILAELTRQFYTIEILPGL
                     AKSAAAQLLALGYRNVEVKAGDGYQGWPQLAPFDAIIVTAGATHVPAPLIEQLKEGGR
                     LVIPVGASSLSQELLQGRKERGRLVTRVVAPVRFVPLIEPKR"
     gene            2058..3038
                     /locus_tag="AC16G0_10795"
     CDS             2058..3038
                     /locus_tag="AC16G0_10795"
                     /inference="COORDINATES: protein motif:HMM:NF014069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HIR5100136.1"
                     /translation="MAKIFIAELLEGEPVTSFFLAKQVQLRQRKKGDPFLTLVLADRT
                     GEVPAVMWDGAEQVAKELTDGDIVKVQGVLGAYQGERQLTLSRLRPAIPDEVSRDDYL
                     PRSPQDSATLLARLRETVEGLQEPHLQRLLRDLLADATFVEALISAPAAKAIHHAVLG
                     GLLEHTASVTGLCRLLADYYPVVDRDLLLTAAILHDVGKTQELTWDRLFDYTDAGRLL
                     GHITLGAMLVEQRIRAIPDFPEPLAQRLLHCILSHHGELEWGSPKRPKTLEALVLHYA
                     EDMDGKINSFLSVSQSHPDPQHPGWTQFNKPLDRYLYFGRGGADPPSERE"
     gene            3083..>3668
                     /locus_tag="AC16G0_10800"
     CDS             3083..>3668
                     /locus_tag="AC16G0_10800"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR5100137.1"
                     /translation="MRDNLPTGRPPVVTLVIIGLNTYVFLKEFLLGAWAGRRFILYYG
                     LIPCSLTGGCHILGRAFSPEVTLFTSMFVHAGFFHFAGNMLYLWIFGNNVEDSMGRFR
                     FAVFYLLSGLGAAFAQILVNPVSRIPMVGASGAISGVLGAYLLLFPQARVLTIIPFGL
                     FTQVAEVPALIVLGFWIVVQLFNGLLTFNFEGGGV"
BASE COUNT          585 a         1130 c         1228 g          725 t
ORIGIN      
        1 ccgccgaatt ccccaagtgg tcctggatgc ggcgccgggg agtccggagg cgtcggatct
       61 ggaggccgcg gcgcgctcgg gacgcgggcc gggagccgag ctcccgaaac tcggtcccga
      121 ggacgtcgcg gcctgtgtgt acacttcggg gaccacgggg cgtcccaagg gtgcgctcct
      181 gacccaccgg aatctcacgg ccaacgtcga gagcttccat gaggtgctga aactggccga
      241 gggggaagtg ttcctcgcgg tcctgccctt gttccacgcc tatgggggga ccgtgctgct
      301 actcgagccg ctgagtgtgg gcgccaccat cgttctggag ccccgctttg tccccgacct
      361 ggtgctccgg gccttggcgg agcatcgggt gagctttttc gcggctgttc cctccatgtt
      421 cgcggtcctg gccaagcatc cccggccggc ggcggacttc agcgcctggc gcctttgcat
      481 ctcggggggg gcgccgctgc cgccgcccgt cctggaggct ttcgaggcga agtacggcgt
      541 ccccatctac gagggatacg gccccacgga gtgtgcgccg gtgctgaccg tgaacccacc
      601 gggcgggacg cgcaaggtgg gatccgtggg tccacccatc ccccgcgtgg aattgcgaat
      661 cgtggatgag cgggggcagc cggtctcgcg gggcgaggtg ggagagatcg tggcccgagg
      721 tcccaacgtg atgcgggggt acctgaatcg gccagaggag accgacgagg tcctccagga
      781 tggatggtat cacaccgggg acctcggtcg cgtggatcag gatgggtact attacatcgt
      841 ggatcgaaag aaagatctga tcctggtggg gggtctgaac gtgtatccgc gtgaggtgga
      901 gctggtcctc gccgaccacc ctgcggtggc tgaggcggcg gtcatcggga ttcccgaccc
      961 cgtccggggt gaggtcgcca gggcgttggt catcctcaag gacgggcagc aggtcggcgt
     1021 gcatgcgctc ttgcagtggt gccggcaacg cttggccaac tacaaggtcc cgcggacgat
     1081 caccatcgtt cccgagcttc ccaggacggc cacgggaaaa gtcctcaagg ctgcccttcg
     1141 gggccgtggt gagtgaatcg ggagatcgct ctcagagacg ctgacgaatc ccggggtgtc
     1201 ttcgacgacg caggcgatcg accgggaggg ctggccgatg tgggggtggg tcggggtcgg
     1261 cttggtgctt gtcgagatgc tgtggctacc gctggccgtt gccgccgagg agtaggcccc
     1321 cttcacacag gcgcgccagc agatggtgga gcgtcagctc aaggggcgcg acatcaccga
     1381 tcctcgtgtc ctctgggcca tggggaaagt ccggcgtcac ctgtttgtcg gccccggcct
     1441 gcggggggag gcgtacaccg accgtgccct tccgatcgcg gaaggtcaga ccatctctca
     1501 gccctacatc gtggcgctca tgacacagct cttggagctg aagggttcgg agcgggtctt
     1561 ggaggtcggg actggatccg gctaccaggc agccattctg gccgagctca cccgacagtt
     1621 ttacaccatc gagatcctcc ccggactggc gaagagcgct gcggcacagc tcttggccct
     1681 tggctaccgg aacgtggagg tcaaagccgg cgacggttac cagggctggc cgcagctggc
     1741 ccccttcgac gcgatcatcg tcacggccgg tgccacccac gtcccggcgc ccctcatcga
     1801 gcagctcaag gaggggggaa gacttgtgat cccggtgggt gcctcatccc tttcgcaaga
     1861 gctgcttcaa ggtcggaagg agcggggccg gctcgtcacc cgtgtggtcg ctcctgtccg
     1921 cttcgttcct ctcatcgagc caaaacgctg agcgcgtatc agccacaatc ttgcattggc
     1981 ttcgacggcc tggctcaggt ccgtgtttgg ccttgcgcac cctgtgtgat tctgctaaca
     2041 aaggattgga ggtgcgcatg gccaaaatct tcatcgccga gttgctggag ggggagccgg
     2101 tcacgtcatt cttcctggcg aagcaggtcc agttgcgcca gcgcaagaag ggggatcctt
     2161 tcctcacgct ggtcctggcc gatcgtaccg gcgaggttcc tgccgtgatg tgggatggcg
     2221 cagagcaggt ggcgaaggaa ctgacggatg gggacatcgt caaggtgcaa ggggtactcg
     2281 gagcgtacca gggggagcgg cagctcacgc tctcgcggct ccgccctgcc attcccgacg
     2341 aggttagccg ggacgattac ctgccgcgga gtccgcagga ttcggccacg ctcctagcga
     2401 ggctgcgcga gacggtggaa gggctgcaag agccccacct gcagcgattg cttcgtgatc
     2461 tgctggcgga tgcaacgttt gttgaagcgc tgatctccgc gccggccgcc aaggcaatcc
     2521 accatgcggt cctaggcggc cttttggagc acacggcatc ggtcacgggg ctttgccgtc
     2581 tgctggccga ctactaccca gtggtggatc gggacctgct cctcacggcg gccattctgc
     2641 acgacgtggg caagacccag gagctcacct gggaccggct cttcgattat accgacgcgg
     2701 ggcggctgct gggtcacatc accctggggg ccatgctcgt cgaacagcgt attcgggcga
     2761 tcccggattt cccggaaccg ctcgcccagc ggttgctgca ctgcatcctg agtcaccatg
     2821 gcgaactgga atggggctca cccaagcgac cgaagaccct ggaagccctg gttctgcatt
     2881 atgccgagga catggatgga aagatcaatt cctttcttag cgtctcccaa agccatcccg
     2941 accctcaaca ccccggctgg acgcagttca acaagccact ggaccgctac ctttacttcg
     3001 ggcgtggggg tgctgatccc ccttccgaga gggagtgagg ggcggccaga gccgaagtgg
     3061 cgcaggatcc cggatgattc ctttgagaga taacctcccc acgggacggc cgccggtggt
     3121 aaccctcgtc atcatcggtc tcaataccta cgtcttcctc aaggaattcc tcttgggggc
     3181 ttgggccggc cggcggttca tcctgtacta cggtctgatt ccgtgcagcc tgaccggggg
     3241 gtgccatatc ctcggccgcg ccttctctcc cgaggtgacg ctcttcacct cgatgtttgt
     3301 gcacgcgggc ttctttcact ttgcaggcaa catgctctac ctgtggatct ttggaaataa
     3361 cgtcgaagac tccatgggga gatttcgctt tgcggtgttc tatctgctct ccggcctggg
     3421 ggcggcgttt gcccagattt tggtgaaccc tgtctcgcgg atcccgatgg tcggggctag
     3481 cggggcgatt tctggcgtgc tgggagccta ccttctttta tttccccagg ctcgggtcct
     3541 caccatcatt ccgttcggcc tgttcaccca ggtcgcggag gtccccgcct tgatcgttct
     3601 gggcttctgg atcgtggtgc aactcttcaa cggtctcctg acattcaact tcgagggggg
     3661 cggcgtcg
//
LOCUS       DBNGZC010000623         3337 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1125414, whole genome shotgun sequence.
ACCESSION   DBNGZC010000623 DBNGZC010000000
VERSION     DBNGZC010000623.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1125414"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="AC16G0_10805"
     CDS             complement(<1..183)
                     /locus_tag="AC16G0_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100138.1"
                     /translation="MSDAQDLQRLQSKVQRLQFLIETSAIISSTLDLDEVLRLVLEKA
                     QSLAKAEASTILFYNPE"
     gene            complement(180..620)
                     /locus_tag="AC16G0_10810"
     CDS             complement(180..620)
                     /locus_tag="AC16G0_10810"
                     /inference="COORDINATES: protein motif:HMM:NF015690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HIR5100139.1"
                     /translation="MQSVAAGPTPGRAGTAMDAELLPGIVLWNVGEYLLAEEEFERVW
                     LTEVGARRRYLRGLIHAAMGFHYMSVSDISSARSKLSSARAILAGFAADFLGLDLHGL
                     RSGIAAARAALERAELDKLFDPAGVPVPRLVPAVAGLAAHEEQT"
     gene            916..1125
                     /locus_tag="AC16G0_10815"
     CDS             916..1125
                     /locus_tag="AC16G0_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HIR5100140.1"
                     /translation="MRSKGTVKWFNDAKGFGFIERPDGEDVFVHYSAIQADGFKTLNE
                     GQAVEFDVVKGPKGLQAANVTKPTE"
     gene            complement(1198..1275)
                     /locus_tag="AC16G0_10820"
                     /pseudo
     CDS             complement(1198..1275)
                     /locus_tag="AC16G0_10820"
                     /inference="COORDINATES: protein motif:HMM:NF024645.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
     gene            complement(1309..1470)
                     /locus_tag="AC16G0_10825"
                     /pseudo
     CDS             complement(1309..1470)
                     /locus_tag="AC16G0_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
     gene            complement(1589..2734)
                     /locus_tag="AC16G0_10830"
     CDS             complement(1589..2734)
                     /locus_tag="AC16G0_10830"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR5100141.1"
                     /translation="MIDRQDAINLLSDLVRIPSVNPRMSPGAGEEEIARYLAERLRAL
                     GLTPTITEVHPRRPNVLVTLQGRPGGPHLLFEAHTDTVSPSHGQLEPFNPRLEGDRLS
                     GRGACDTKASVAAMLLALQQLLPCRERETSVSLAFTMGEELGHEGVTHLLGSGFRADA
                     AVVGEPTGLDIVTAHKGALRWRLVASGRAAHSSNPQEGVNAIVKMALVVRAIHDRLAP
                     QISRRTHPLLGAPTISVGRIEGGLQVNVVPDRCSIDVDRRTLPGETWAQVRGEVEALI
                     ASLQTEDPELRVTIEEPYVNLGSLETPPDAPIVRLAQEAVRRIDGQHPIRAVAYGTDA
                     AELSTAGTACVVLGPGSVEQAHTSSEYVEIQQVVKAAAIYGEIMQGF"
     gene            complement(2798..>3337)
                     /locus_tag="AC16G0_10835"
     CDS             complement(2798..>3337)
                     /locus_tag="AC16G0_10835"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5100142.1"
                     /translation="SIATDAERLIQTAIQAFGRIEILVNSAAIFHRTPFGTLTAEEWD
                     RFLNVNLKAPFLLCRQAGEIMLRQGRGKIINLADIAGMKAWAEYIPYSVSKAGMISLT
                     QGLAKALAPSIQVNAIAPGAILLPEGTTPEEREQAIRRVPLKRLGSPEDIARAAVYLI
                     ENDFITGEVLTLDGGQHLL"
BASE COUNT          622 a         1058 c         1046 g          611 t
ORIGIN      
        1 ctccggattg tagaacagga tggtgctggc ctccgccttg gcgaggctct gggccttttc
       61 caggaccagg cgcaacacct cgtcgagatc cagggtggag ctgatgatgg cgctggtctc
      121 gatgaggaat tgcagccgtt gaaccttgga ttggagacgc tgaagatcct gtgcgtcgct
      181 cacgtttgtt cctcgtgggc cgccaggcca gcaaccgcgg ggacgaggcg cggaaccggc
      241 acacctgcgg gatcgaagag cttatccaac tcggccctct ccagggccgc ccgcgctgcg
      301 gcgatgcctg acctgagccc gtggaggtcc agaccgagaa aatcagcggc gaagccggcc
      361 aggatggcgc gggccgagga cagcttcgaa cgagcggatg agatgtcact cacgctcatg
      421 taatggaagc ccatggcggc atgaatcagc ccgcgaagat accgccgccg cgcccccacc
      481 tccgtcagcc agacgcgttc gaactcctcc tcggccagaa gatattcgcc gacattccag
      541 aggacaatac ctggtagcaa ctccgcatcc atcgccgtgc ctgctctgcc gggcgtgggt
      601 cccgccgcga cagactgcat cggatccgcc tcgcccggcc tcgaggtcgc ctccacccgc
      661 gggaaaccgg acggtatcag acgaccccca acccgtcaag gagacacagg aatgagagtt
      721 tctgcaagat aatccttgac acctgggggg aatgacgtta ttctcccgcc cggttcggta
      781 gtccacgttc cgttggttgt ttccgtaggg aaggaccgca ggagcagacg ccgctccggc
      841 ggtttttctg tttttgggga caaaccaccc cagtgtgggg tggcgtagcg ccagaaggga
      901 aggaggtgtt gtcagttgcg aagcaaagga actgtgaagt ggttcaacga tgcaaagggt
      961 tttggcttca tcgagcgacc tgacggtgag gatgtgtttg tgcactattc cgccatccag
     1021 gccgacggct ttaagaccct gaacgagggg caggccgtcg aattcgacgt ggtgaagggt
     1081 cccaagggcc ttcaagcggc caacgttacc aagccgacag aataaatcgg catcgtcgta
     1141 accaaaaaag cccccaggga aaacccctgg gggctttttg cactcaagaa gacgaactta
     1201 aacgggatcc gacatcttct tgccgcagga tgaacaaaat actgcgtccg tcggcacact
     1261 ctttccacag tggggacaaa acttccccgc accgccagtg acctcgccct tgggggcggc
     1321 aggttccggt tccttcttgt ctgcagcctc tttgaatcct cgaatggcct ttcccaggga
     1381 gctcccgacc tctggcaacc gcccggcccc gaagaccagc agggcgatca ccaggatcac
     1441 gatgagctcc ggcataccca gtccgaacat agtcccccct ttcgcttacc gtaaatattg
     1501 cgcaaaacta taccatgcta tcatttcgac cgccacacct atcaagcgaa gagacacgga
     1561 gaagctccgc cccgaaccat cggacgcctc agaaaccttg catgatctcc ccatagatcg
     1621 cggccgcctt cacgacctgt tggatctcca cgtattccga gctggtgtgg gcctgctcga
     1681 cgctgcctgg ccccaggacc acacatgccg tcccggcggt ggagagttcc gccgcgtcgg
     1741 ttccgtaggc cacagctcgg atggggtgct ggccgtcgat ccgtcgaacc gcctcctgtg
     1801 ccagccggac gatcggtgcg tccggaggtg tctccaggct gccgaggttc acatacggct
     1861 cctcgatggt cacccgtagc tctggatcct ccgtctgcaa ggaggcgatc agcgcctcca
     1921 cctcgccccg cacttgtgcc caggtttcgc ctggaagcgt ccgacgatcc acgtcgatgc
     1981 tgcaccggtc cgggaccacg ttcacctgca gacctccctc aatccgcccc acgctgatgg
     2041 tgggcgcacc caggaggggg tgcgttcgcc gggagatctg cggggcgagg cggtcgtgga
     2101 tggcccgaac gaccagggcc atcttgacga tggcattcac gccctcctgt ggatttgagc
     2161 tgtgggcggc ccggccggag gccaccagcc tccagcgcaa ggcacccttg tgcgcggtga
     2221 cgatgtccag ccctgtgggt tcgccgacta cggccgcatc ggcccggaac cccgaaccca
     2281 gaaggtgtgt caccccttcg tgtcccagct cctcacccat ggtgaaggcc agagaaacgc
     2341 tcgtctcccg ttctcggcac gggaggagtt gctgcaaggc gaggagcatg gccgccaccg
     2401 acgccttggt gtcacaggcg ccgcgcccag acaggcggtc accctccagc cgtggattga
     2461 atggctctag ctggccgtgg gatggggaga ccgtgtccgt gtgggcctcg aacaggaggt
     2521 gcggaccccc gggtcggccc tggagcgtga ccaagacatt ggggcgccgc ggatgcacct
     2581 cggtgatcgt cggcgtcagg cccagcgcgc gaagccgttc ggccaggtac cgggcgatct
     2641 cctcctcgcc tgcgccgggg ctcatgcgag ggttcacgct ggggatccga accaagtcgg
     2701 agaggagatt gatggcgtcc tggcgatcga tcatgccgct cggctcccgg ttcaagggtc
     2761 aacgctcaag gtgtggggtc ccgcgaacga ggtccgttca gaggagatgc tggcccccgt
     2821 ccagggtcag cacctctccg gtgatgaagt cgttctcgat aaggtagacg gcagcccggg
     2881 cgatgtcctc gggtgagccg aggcgtttca gggggacgcg ccgaatggct tgctcccttt
     2941 cctcgggcgt ggtcccttca ggcagcagga tggcacccgg ggcgatggca ttcacctgaa
     3001 tggagggggc cagcgctttg gccagcccct gagtgagtga gatcatgccc gccttcgaaa
     3061 cggaataggg gatgtactcg gcccaggctt tcatgccggc gatatcggcc aggttgatga
     3121 tcttcccccg accctgtcga agcatgatct ctcccgcctg cctgcagagc aagaatgggg
     3181 ccttcaggtt cacgttcagg aagcgatccc attcctccgc cgtcagcgtc ccgaagggtg
     3241 tccggtgaaa gatggcggcg ctgttcacca ggatctcgat ccggccgaag gcctggatgg
     3301 cggtctggat gaggcgctct gcatctgtgg cgatcga
//
LOCUS       DBNGZC010000624         2042 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2401917, whole genome shotgun sequence.
ACCESSION   DBNGZC010000624 DBNGZC010000000
VERSION     DBNGZC010000624.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2401917"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1901
                     /locus_tag="AC16G0_10840"
     CDS             <1..1901
                     /locus_tag="AC16G0_10840"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HIR5100143.1"
                     /translation="QVGLVAGLSAALATLWGVVASQRRGRGLCVDVSEWEAFTHLLYE
                     HTAQLSDGKLPADRRRRAGAVITVVGGLILCLPCSDGWVLASPREDHQFKLWGELIED
                     PAWAARPEFADPVLRERHGWEIYERSAAWTRDRLKAEVYLAAQGKKLPCFPVSQMHDL
                     AELEQLKHRRFWTEMDHPVIKGLQYPGLPARIEGVQELPSRGAPSPGEHRLDVIEELS
                     RPPVKSGMLRDLRSSAGPARGYQSVLDGVRVLDFSWVVAGPFCTKLLALMGGEVIKVE
                     SARRAQYKDRGAWFSVLNNSKKSCTLNLATDQGKAIVKQLVRASDVLVENFSTGVMDR
                     LGLGYEALRAIKPDLIYVSSSGVGRSGPGRNWLAYGSLLQGLSGWTSLFSKPNPWMEG
                     MGIAPSWTDPLTGLWEALIIQAALLHRARTGTGLYVDLSMLECTIPAMGDVFLRAAAG
                     RLQTKRQSAESCALAAPHGIYPCRDEDSWIAISVGSDGEWEGLCKTFENPPWSEENAI
                     RTPEGRRKHRERLNGLVAGETSRRDVKDLFFRLQEAGVAAGPCYNLRDLVEDSHMQSR
                     ALFQRVPLADGRVQLTTGLPWREESEWKGRIERAPALGEHNEYVFRKLLQLSDEEYER
                     YRTEGVFE"
BASE COUNT          360 a          605 c          718 g          359 t
ORIGIN      
        1 atcaggtagg tctggtggcg ggactttccg ccgcgctggc gaccctctgg ggcgtggtcg
       61 ccagtcagcg gagggggcga gggctatgcg tggacgtgtc tgaatgggaa gcgttcacgc
      121 atctcctgta cgagcacacg gcccagctct ccgatggcaa gctccccgcg gaccgcaggc
      181 gtcgggcggg agcggtcatc actgtcgtgg gcgggctgat cctgtgtctg ccctgctcgg
      241 atggctgggt gttggcctcg ccgcgggagg atcaccagtt caaactctgg ggggagctga
      301 tcgaggaccc ggcctgggcc gctcgccccg agttcgccga cccggtcctc cgcgagcgac
      361 acggctggga aatctacgag cgctcagccg cgtggacccg agaccgactg aaggccgagg
      421 tgtacctggc agcccagggg aagaagctcc cgtgctttcc cgtgagccag atgcacgact
      481 tggccgagct ggagcagctc aaacaccggc gcttctggac agagatggac caccccgtga
      541 tcaagggcct tcaatacccc gggcttccgg ccagaatcga gggggtgcag gaattgccgt
      601 cccgcggggc gccgtcgcca ggggaacaca ggctggatgt catcgaggag ctgagccgtc
      661 cgccggtgaa atccgggatg ctgcgggacc ttcggtcgtc cgcgggtcct gcccgcggtt
      721 accagagcgt gctggacggc gtgcgcgtcc tcgatttcag ctgggtcgtg gcagggccct
      781 tctgcacgaa gctgctggcc ttgatgggcg gggaggtcat caaggtcgag tccgcccggc
      841 gcgcgcagta caaggaccga ggcgcctggt tctccgtgct caacaactcg aagaagagct
      901 gcacgctgaa cctcgcgacc gatcagggca aggcgattgt gaagcagctc gtccgcgcga
      961 gcgatgtgct tgtcgagaac ttttccaccg gggtaatgga tcgcctcggc ctcggctatg
     1021 aggccctcag ggccatcaag ccggatctca tctatgtttc ctcttcgggc gtcggtcggt
     1081 ctggccccgg tcgaaactgg ctggcctacg gcagcctcct gcagggcctg agcggctgga
     1141 cctccctgtt cagcaagccc aatccgtgga tggaggggat ggggatcgcg ccgagctgga
     1201 ccgatccgct cacgggattg tgggaagccc tcattatcca ggcagcgctg ctgcaccggg
     1261 cacggaccgg gacggggttg tacgtggacc tgtctatgct cgaatgcacg atccccgcca
     1321 tgggcgatgt cttccttcgt gccgcggcag gtcggctgca aacgaagaga cagtcagctg
     1381 agtcgtgcgc cctcgcggcg ccccatggca tctatccatg ccgcgacgag gattcctgga
     1441 tcgccatctc cgtcgggagt gacggcgagt gggaggggct ctgcaagact tttgagaatc
     1501 cgccgtggtc tgaagagaac gcgattcgca cgccggaggg gagacggaaa catcgcgagc
     1561 gcctaaatgg gttggtggcc ggtgaaacat cccgaaggga cgtcaaggac ctcttcttcc
     1621 gcttgcagga ggccggggtg gcggccggac cgtgctacaa cttgcgggat ctcgtcgagg
     1681 attcccatat gcaatcacga gcccttttcc agcgcgtccc tctggccgac gggagggtcc
     1741 agctcaccac gggactcccc tggagggagg agtccgagtg gaagggaagg atcgaacggg
     1801 cgccggcatt gggcgagcac aacgaatatg tctttcgcaa attattgcag ttgtccgatg
     1861 aggagtacga gaggtaccgg accgaagggg tgttcgagta ggcgaggaat tatcgaagga
     1921 ggagcatgga ggagcctatg agctcccgga cagggcgcct gcccttcagg ggagtgttcc
     1981 caattgcacc gaccccgttc acggaagcgg gggaccttga cctggacggc cagcggcgcg
     2041 tg
//
LOCUS       DBNGZC010000625         5407 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2009265, whole genome shotgun sequence.
ACCESSION   DBNGZC010000625 DBNGZC010000000
VERSION     DBNGZC010000625.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5407)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5407)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5407
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2009265"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="AC16G0_10845"
     CDS             complement(<1..841)
                     /locus_tag="AC16G0_10845"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5100144.1"
                     /translation="MAGDPVRSGRVAAALSGVSVGLADLFNLLISAILLAGIYTTMSI
                     GMTVIYGVMKMVNLAHAGFMMLGAYFAYELSQRTPIDPLLAAVLAVPVFFLLGIAAYW
                     SMVRRVPVADVPTLPSLLLLFGLWLVLQNIGYAIWGADDQSILTPLTMATFRFSGVTV
                     PVIRLIVFGIALVSLVLLHLCLTRTWFGRSVRALTQNREAGQLVGIDTQRTAMLTFGL
                     GIAFTGLAGGLLATLYSFTPDFGGSFMLRSFVIIVLGGLESFAGVAIGAIIVALIETF
                     SILL"
     gene            complement(932..2137)
                     /locus_tag="AC16G0_10850"
     CDS             complement(932..2137)
                     /locus_tag="AC16G0_10850"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5100145.1"
                     /translation="MAAIGGWAGESLAGQAASCPDSVRVGLIFPQTGREGRPGIYQLE
                     GIRLAIEQINAKGGVDVKACGKRLPVKEVAYDDQSDQGRSVQLAERTMSSDNVVAVLG
                     GYSTVLGEAQSVVADRYQVPWITPGAAASAIFSQGRQWVFGTLTPVDVLGYTTMKFLG
                     SLVDQGKLDKGLKLAIAVENTDHGKDYVDGVANWIKDHPGYFTIAFNESFQLGGTDFS
                     GLLQRVKAANGDIFLSDAHLQDYITMHRQYTQLGLYHRMVSYGARGPEEPARKALGRA
                     ADFIFAGTWWEKGLPYPQVKAFAQAYKKKYNHDPDNFYPATAYEGMRMLAAAIEAAGS
                     LDRTAVRDALRKVRLTDSLLPGQVLEFQKNGQAAAPFVIVQNKPEGRVDFVYPEDTKT
                     GEVVTKIPR"
     gene            complement(2579..3856)
                     /locus_tag="AC16G0_10855"
     CDS             complement(2579..3856)
                     /locus_tag="AC16G0_10855"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR5100146.1"
                     /translation="MLLFTTFCGLIALSVPIGFSIGLASALALVYRGDMPLTLLAQKM
                     FTGLDSFPLLAVPFFILAGLFMETGGISARLVRFASVLVGHIRGGLAHVVIVATIFFS
                     GVSGSSAADTAAIGSIMIPSMVKKGYPRPLSTAIVAAAGGMGVNIPPCIIMVLYGVAA
                     NVSIGYLFSAGFLPGFLSGLSLMILVYFIAKRQNLPREAKATLRQMLRAGWDAIPPLL
                     MPVIILGGILTGVFTATESAVVAVAYGVFLSMVVYGELHFRDIPRILVATAKLTGMVM
                     LVVGMCITFGWIVTVEQVPQAIIQWMLGVSQNPIVFLFFVNILMLIVGMFLDVTPAII
                     TFTPILYPVARQLGIDPVHFGIVMVTNLGVGFVHPPVGSCLIVATAIGEITIDEVIRP
                     ILPFIGVMIIALLLITYWPGMTLFIPRLLGYRG"
     gene            complement(3878..4318)
                     /locus_tag="AC16G0_10860"
     CDS             complement(3878..4318)
                     /locus_tag="AC16G0_10860"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR5100147.1"
                     /translation="MTILVFLTVIYRYFLLAPISWGEEMSRFLFIALSMLGAALAVKD
                     RSHFTITILTSRFPAFVRLWLQVGIALGISLLLGIVIDKGWGLTLLNRNQESPALGVP
                     MSIPYSAVPVGAMLMLVFLWLDLLIHWRGVRGTEEGTGDSRAEV"
     gene            complement(4487..>5407)
                     /locus_tag="AC16G0_10865"
     CDS             complement(4487..>5407)
                     /locus_tag="AC16G0_10865"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR5100148.1"
                     /translation="APPGATVTKFASVLAESHAHHKSYVFFADRVKELTGGKLIINVY
                     PNSQLGNEREYVEFLQTGQIEFGRVATSVLGPFIPQFQAFDLPYLFKSKQDMFRAADG
                     QLGNLLLKLLAEKLNIKGLGFFDDGSRCLYSSKRPINMPADFKGMKVRTMENQLMIKT
                     FNALGAAATPLPYGELYSALQQGVVDAGEGPVQSYWAMKHYEVAKYFSYTDTFISPAL
                     PMVGLKWFNAQPKEFQQAIDQAAKEMIVKERQLMAEQEKTATDDLKAKGVIFTEVKDK
                     AAFMKAVESIHKEYEARLGKDLLDMARNPK"
BASE COUNT         1091 a         1656 c         1756 g          904 t
ORIGIN      
        1 ccagcaaaat gctgaaggtc tcgatgagcg ccacgatgat cgccccgatg gccacgccgg
       61 cgaacgactc caatccgccc aacacgatga tgacgaagga ccgaagcatg aaggatcccc
      121 cgaagtccgg cgtgaaggag tagagcgtgg ccaggagccc gcccgccagg ccggtgaagg
      181 cgatgcctag accgaaggtg agcatggccg tccgctgggt gtcgatgccc acgagttggc
      241 ccgcctcgcg gttctgggtg agtgcccgca ccgatcgccc aaaccaggtt cgggtcagac
      301 acaggtgcag caacaccagg gagaccagcg cgatcccgaa gacgatcagc cggatgaccg
      361 ggacggtgac gccgctgaac cggaatgtcg ccatggtgag cggcgtgagg atggactgat
      421 cgtccgctcc ccagatcgcg tagccgatgt tctggaggac cagccacagg ccgaacagca
      481 agagcagcga ggggagcgtg ggcacgtcgg ccaccggcac ccgtcgcacc atgctccaat
      541 acgccgcgat ccccaacagg aagaagaccg gcacggccag caccgcggcc aggaggggat
      601 cgatcggcgt ccgctgcgac aactcgtagg cgaagtaggc gcccagcatc atgaagcccg
      661 catgggcgag gttcaccatc ttcatgacgc cgtagatcac cgtcatcccg atggacatgg
      721 tcgtgtagat gcccgcaaga aggatggccg agatcagcag gttgaacaga tcggcaagac
      781 ccacgctcac ccccgagagg gccgcggcaa cccgacccga cctcacagga tcacccgcca
      841 agacagcaaa agcgccaaga atcaacggag aggccagacg gcttcggcct tggcgttctt
      901 ggcgccttgg cggttcaagc gtctgcttgg atcaccgcgg gatcttcgtg accacctcac
      961 cggtcttggt gtcctcggga tacacgaagt ccacccgacc ctccggcttg ttctggacga
     1021 tcacgaaggg tgcggcggct tggccgttct tctggaactc caggacctgg ccaggcagga
     1081 gcgagtcggt cagtcggacc ttgcggaggg cgtctcgaac ggcggtgcgg tcgagtgacc
     1141 cggccgcctc gatcgccgcc gccagcatcc gcattccctc ataagccgtc gcggggtaga
     1201 agttgtccgg atcgtggttg tacttcttct tgtaagcttg cgcaaaggcc ttcacctgcg
     1261 ggtaggggag gcctttctcc caccaggtgc cggcgaagat gaagtcggcg gctcgcccca
     1321 gagccttccg ggctggctcc tcgggaccgc gggcgccgta gctcaccatc cggtggtaga
     1381 gtccgagttg cgtgtactgc cggtgcatgg tgatgtagtc ctgcaggtgg gcatccgaga
     1441 ggaagatgtc cccgttggcc gccttcaccc gctggaggag cccggagaag tccgtcccgc
     1501 cgagctggaa ggactcgttg aaggcaatgg tgaagtagcc tgggtggtcc ttgatccagt
     1561 tcgccacccc atccacgtaa tccttgccgt ggtcggtgtt ttccaccgcg atggcgagct
     1621 tcaagccctt gtccagtttc ccctggtcta ccagggagcc caggaacttc atcgtggtgt
     1681 agccgaggac atccaccggc gtgagggtcc cgaagaccca ctgtcgcccc tgactgaaga
     1741 tggcgctggc cgcggccccg ggggtgatcc atggcacctg gtagcggtcc gccacgaccg
     1801 actgggcctc gccgaggacg gtggagtatc cgccgaggac ggcgacgacg ttgtccgagg
     1861 acatcgtccg ttccgcgagc tgcaccgagc ggccttgatc cgactggtcg tcgtaggcga
     1921 cctcttttac ggggagccgc ttgccgcacg ccttgacatc gacgcccccc ttggcattga
     1981 tctgctcgat ggccagccgg atgccttcga gctggtagat ccccgggcgg ccctcgcgcc
     2041 ccgtctgggg aaatatcagg ccgacccgga ccgaatccgg gcaagacgca gcctgcccgg
     2101 ccaggctttc cccagcccag cccccgatcg cagccaccgc cgtcagacaa gcaacgagat
     2161 accaccaccc tcgtcgagac agacctcgca acctcacgtt cctcatcatg tggagtcccc
     2221 ctcccctgag ttcaagcgag atgtgtcgag cgaagtcccc tggttgacgc tacgccacac
     2281 caatcgccgc ggcagttatg tgacgggagc aggccatccc aggatgacct gcgagaagca
     2341 gaggatcatg cgacggctgg gatgcgaact gccccagtgt gctctctggc gaaaggagcg
     2401 gcaggttgcc gctgagcgcg ccgactttct gcgaatgcct caggctacta tgctaggcca
     2461 attgtggcaa gtttcacatc tatggagcca tcagcggcag aatggttgca gcggggtctc
     2521 gcattccaac gagtctggat caagtcagcg ggcgatcgtc cggcggcagg tggcgtgact
     2581 agcccctgta tcccaggagt ctcgggatga acagggtcat tccgggccag taggtgatca
     2641 agagcagggc gatgatcatc acgccgatga agggaaggat cggccgaatc acctcgtcaa
     2701 tcgtgatctc accgatcgcc gtggcgacaa ttaggcacga cccaaccggg gggtggacga
     2761 agcccacacc cagattggtg accatgacga tgccgaagtg cacggggtcg atgcccagct
     2821 gccgcgcgac cgggtagagg atcggggtga aggtgattat ggctggggtc acatccagga
     2881 acatgccgac gatcagcatc agtatgttca cgaagaaaag gaacacaatg ggattctggg
     2941 agacgcccag catccactgg atgattgcct gcggtacttg ttcgaccgtg acgatccagc
     3001 cgaaggtgat gcacattccc acgaccagca tgaccatgcc tgtcagcttg gctgtggcca
     3061 ccaggatgcg cggaatgtct cggaaatgga gctcgccata caccaccatg gagagaaaga
     3121 ccccgtatgc caccgcgacc accgcggatt cggtggcggt gaagacaccg gtgagaatgc
     3181 cgcccaggat gatgactggc atcagcagcg ggggtatggc atcccagccc gccctcaaca
     3241 tctgccggag tgtggccttg gcctcacggg gcagattctg gcgcttggcg atgaagtaca
     3301 ctaggatcat gagtgacagc cccgagagga atccgggcag aaagccagcg gaaaaaaggt
     3361 agccgatgga gacgttggcc gccacgccgt acaggaccat gatgatgcag ggcgggatgt
     3421 tcacccccat cccgcccgcc gcggccacga tggccgtgga taacggcctc gggtagccct
     3481 tcttcaccat ggacgggatc atgatggagc cgatggcagc cgtgtcggcg gcggaggagc
     3541 cggacacacc cgagaaaaaa atggtggcga cgatgacgac gtgggcgagt ccgccccgga
     3601 tgtggcccac cagcacgctg gcgaagcgca ccagcctggc cgagataccg cccgtttcca
     3661 tgaagagtcc agccaggatg aaaaagggaa cagccaggag cgggaaggag tccagccccg
     3721 tgaacatctt ctgggcgagg agggtgaggg gcatgtcgcc ccggtagacc agggccagcg
     3781 cgctggccag gccgatggag aaaccgatgg ggaccgacaa ggcgatcagc ccgcagaatg
     3841 tcgtgaagag gagcaaggag accagcccgt acctccctca cacctcagcc cgggagtcgc
     3901 cggtaccctc ttcggtcccg cggacaccgc gccaatggat gagcaggtcc agccacagga
     3961 acaccagcat cagcatggcg cccaccggga cggcggaata aggaatgctc atgggcaccc
     4021 cgagggcggg cgactcctgg ttccggttca gtagcgtcag cccccacccc ttatcgatca
     4081 cgatgccgag cagcaggctt attcccaggg cgatcccgac ctggagccag aggcgtacga
     4141 acgccgggaa acgcgacgtg agaatggtga tcgtgaaatg cgagcggtcc ttcaccgcga
     4201 gtgccgcgcc cagcatggag agggcgatga agaggaagcg agacatctcc tcgccccagc
     4261 tgatcggcgc gagcaggaag tagcgataga tgaccgtgag gaagacgagg atcgtcatca
     4321 cggcaaggat cgtgccggcg acgaatgccg tgaggcggcc gagccagacg tggaggaatc
     4381 ggatgggggc gatggcacac ctccgcggta gaaaccggtg caggaagcga tacagaacag
     4441 aagctcccgg agggtcgcgg ctgccctccg ggagccggtg gtgtcgttac ttggggttgc
     4501 gcgccatgtc gagcaggtcc ttcccgagcc gcgcctcgta ctccttgtgg atcgactcga
     4561 cggccttcat gaaggcggcc ttgtccttca cctcggtgaa gatcacgccc ttcgccttga
     4621 ggtcatcggt ggcggtcttt tcttgctcgg ccatcagctg acgctccttg acgatcatct
     4681 ccttggcggc ctggtcaatg gcctgctgga actccttcgg ctgcgcgttg aaccacttga
     4741 ggcccaccat aggaagcgcc ggcgagatga aggtgtcggt gtacgagaaa tacttcgcca
     4801 cctcgtaatg cttcatggcc cagtacgact gcactgggcc ctcgcctgcg tccaccacgc
     4861 cctgctggag cgccgagtac agctcaccgt aggggagtgg agtcgcggcc gcccccaggg
     4921 cgttgaaggt cttgatcatg agctggtttt ccatggtccg gaccttcatc cccttgaaat
     4981 cggccggcat gttgatcggc cgcttcgagc tgtagaggca gcggctgccg tcgtcgaaga
     5041 agcccagccc cttgatgttc aacttctccg ccagcagttt gagtaggagg ttgccgagct
     5101 gcccgtctgc cgcgcggaac atgtcctgtt tgctcttgaa caggtagggg agatcgaacg
     5161 cctggaactg cgggatgaag ggcccgagca cagaggtcgc gacgcgcccg aactcgatct
     5221 ggcccgtctg aaggaactcg acgtattccc gctcattccc gagttgggag ttggggtaca
     5281 cgttgatgat gagcttgccg ccggtgagct ccttcacccg gtcggcaaag aagacgtagg
     5341 acttgtggtg ggcgtggctt tccgccagga cggatgcgaa cttggtaact gtggcgcctg
     5401 gaggtgc
//
LOCUS       DBNGZC010000626         6106 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_350598, whole genome shotgun sequence.
ACCESSION   DBNGZC010000626 DBNGZC010000000
VERSION     DBNGZC010000626.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_350598"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /locus_tag="AC16G0_10870"
     CDS             <1..1090
                     /locus_tag="AC16G0_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HIR5100149.1"
                     /translation="DLASTLARELADVDRLGGFFDIIGQDPVISRVKLIAEPWDLGEG
                     GYQVGNFPVLWAEWNGQYRDTVRAYWKGDEGQAAALGYRLTGSSDLYGKGGRRPYASI
                     NFVTAHDGFCLHDLVAFNTKHNEANGEGNRDGTDDNRSWDCGIEGPTDDPVILDLRTR
                     QARNFLATLFLSQGVSMLCGGDEIDRTQQGNNNAYCQDNEIGWLDWTLDKRRRDLLAF
                     TRSLIGLRRRHPVLRRRQFFYGRRIRGSEVKDLAWFRPDGKEMTEEDWTNPQTRCLGL
                     RLAGDAIEEVDVKGEPILDDTFLILLNAHHESVSFVLPAHRPGVKWELVLDTRTVDGR
                     RLHRPMKGGQPYGLEGRCLACLRLGGES"
     gene            1185..1571
                     /locus_tag="AC16G0_10875"
     CDS             1185..1571
                     /locus_tag="AC16G0_10875"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5100150.1"
                     /translation="MVKTASILVVDDDPEMRALVLDVLRNEDYEVVEAKDGTEAVLAL
                     RTRQFDVILMDKNMPGPSGLDLLPGFQRVCPRSRIIMMTAFGDVPSYMEALEKGAVEY
                     LFKPFRMEEMKAAIQKALGAKADSLT"
     gene            1611..3176
                     /locus_tag="AC16G0_10880"
     CDS             1611..3176
                     /locus_tag="AC16G0_10880"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5100151.1"
                     /translation="MRNLTNRLVILTMLALMIITGVNDYRRVLRERERLVGQSEEDVR
                     IFAETLALAVSRNVRWGRTTAELKELLDDILARPGLVAVAIYGPEGQVIAENVATDVP
                     PPAVDRVVRNTLTTKEPALGTLEMQSARVVRYVQPFRWPGGRTGAIEVRQTLEGMERE
                     FQRAVRESILSRLVVLTLFVLSIVVLTRWSIGRPIRALIAAARAVGRGDLDQRIDVKR
                     RDEIGQLAEEFNRMAENLQSAHEEILRQGEERLRLEGEAQQAQKLAAVGMLAAEVAHE
                     IGTPLNIISGRAEVLGRVVTPDQPEHRNLDVILLQTQRISKIIRALLDYTRPRRPTFR
                     TEDVVPIIGRVSDLLLERSRRRGVRIVLDLPVGLPQIQADPDQLQQLFLNLLLNALDA
                     SPQGETVLVTVGPEPVLPAEDRAGIVRGRLEGPSLAIHVVDAGKGLTAEQLNHAFEPF
                     FSTKSQGQGTGLGLPIVEQIVRAHHGEIEMLSIPGRGTEVILRLPLVPEPGTTAPTAA
                     THHAIRKADANGQ"
     gene            3166..>6106
                     /gene="treY"
                     /locus_tag="AC16G0_10885"
     CDS             3166..>6106
                     /gene="treY"
                     /locus_tag="AC16G0_10885"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HIR5100152.1"
                     /translation="MDSDPYLELQGDPVLHPRIPVATYRLQFNPSFRFETAGRLVPYL
                     EALGISDVYASSYLAARPGSLHGYDIANHNALNPEIGTEEEYHGFVGALQAAGMGQIL
                     DVVPNHMGIAAACNPWWNDILENGPGSPYAEFFDIDWDPVKRQLANKVLLPILGDQYG
                     RVLDGGELRLEYAEGGFSLRYYDTRLPIAPRSVIRILGHRLEELTAALGEDSPHLQEY
                     LSILTALTNLPGRTETSPERVRERRREKEVIRRRLTDLITACEPVRTSIEETIRIFNG
                     KTGDPRSFDLLDRLLDEQVYRLAYWRVAAEEINYRRFFDINDLAAIRMENPAVFRAAH
                     QLIFRLVEEGKVTGLRLDHPDGLFDPPQYFHALQRERLSQVLRARPLSEGAADRLDRE
                     TLVARGVAQFDSTCQPDPTHLGCRPLYLLAEKILARGERLPLQWAIHGTTGYEFLNIA
                     EGLFVDSENEKAMTAAYTVFTGQRKPFADLVYQSKQLILQVSMSSELNVLGHTLDRLS
                     ERSRYSRDFTLNSLTHALQEVIACFPVYRTYIDGRGAEVVLQDRACVEVAVAFAKRRN
                     PTTNVSVFDFVRDVLLLRYPESADASYRRDQLAFVQKFQQVTAPVTAKGVEDTAFYRY
                     NRLVSLNEVGGEPDRFGISLEEFHKQCLARQEKWPNGLSAGSTHDTKRSEDVRARIHV
                     LSEIPREWRAAVGRWHRWNRRHLAEVDGRPAPDRNDEYLLYQTLVGAWPLAPMGPEEV
                     SAFTARIQDYLLKAAKEAKLNTSWINPNEAYDDALQTFAARILDPSPTNRFLTDFTAF
                     HPFVARLGMVNSLAQTTLRITAPGVPDFYQGSELWDFSLVDPDNRRPVDFATRSSLLE
                     DLRRRIATGDLVGLAREVVEQWTDGRVKLYTIHRALMYRRTAADLFQRGDYVPLPAGG
                     QAALHLCAFARRLATRTAITVVPRLSASLTDNGARLPLGRDVWGDTWVALPRDLAGGP
                     YRNLFTGE"
BASE COUNT         1066 a         1983 c         2047 g         1010 t
ORIGIN      
        1 cgacctcgcc tccaccctag ctcgcgagct ggccgatgtc gaccgcctgg ggggcttctt
       61 cgacatcatc ggccaggacc cggtcatcag ccgtgtgaag ctcatcgccg agccatggga
      121 cctgggcgag ggcggctacc aggtcggcaa ctttccggtc ctgtgggccg agtggaacgg
      181 gcagtaccgg gacacggtcc gggcctactg gaagggggac gagggccagg ctgccgccct
      241 gggctatcgc ctcacaggct cgagcgatct gtacgggaag ggcggacggc ggccgtatgc
      301 cagcatcaac tttgtgacgg cccatgacgg cttctgcctg cacgacctgg tggccttcaa
      361 cacgaagcac aacgaggcca acggggaggg aaaccgggac gggaccgacg acaaccggag
      421 ctgggactgc ggcatcgagg gaccgacgga cgatccggtc attctcgact tgcggacgag
      481 gcaggcccgg aacttcctgg cgaccctgtt tctctcgcag ggggtgtcca tgctgtgcgg
      541 aggcgacgag atcgatcgga cccagcaggg gaataacaac gcctactgtc aggacaacga
      601 gatcggctgg ctcgactgga ccctggacaa gcgacggcgc gacctcttgg ccttcacccg
      661 ctccctcatc ggactccgcc gccgccatcc agtcctgcgc cggcgccagt tcttctatgg
      721 aaggcggatt cgaggttcgg aggtgaagga tctggcctgg ttccggcccg acggcaagga
      781 gatgaccgaa gaggactgga ccaatccgca gacccggtgc ctgggcctcc ggttggcggg
      841 ggacgccatc gaggaggtgg acgtcaaggg cgagccgatc ctggatgata cctttctcat
      901 cctgctgaat gctcatcacg aatccgtttc cttcgtcttg cctgcccatc ggcccggcgt
      961 gaagtgggag ctggtcctgg acacgcggac ggtcgacgga cgccgactgc accgccccat
     1021 gaaagggggc cagccctacg gcctggaggg ccgctgtctg gcctgcctac gactgggggg
     1081 cgaatcatga cgcagggtcc ccgtacctcg ccaatgggca cgccgagcgg gcgggcatcc
     1141 gacccgcttc ggccgagcgt tgcaggtgaa aggagggtaa gaccgtggtg aagacggcga
     1201 gcatcttggt ggtggatgac gatccggaga tgcgcgcact cgtcctggac gtcctccgga
     1261 acgaagacta cgaagtggtt gaggccaagg acggcaccga ggcggtgctg gcccttcgca
     1321 cccggcagtt cgacgtcatc ttgatggaca agaacatgcc cggacccagc ggcctggatc
     1381 tcctccccgg atttcagcgg gtgtgcccca ggtcgcggat catcatgatg accgccttcg
     1441 gagatgtccc gtcctacatg gaggcactgg agaagggcgc ggtcgagtat ctcttcaagc
     1501 cgtttcggat ggaggagatg aaggcggcca tccagaaggc cttgggtgcg aaagccgact
     1561 ccctgacgtg atccgccgcg atccgttcgc ggaaacgggg gcccgaccgt atgcgaaacc
     1621 tgacgaaccg tctggtcatc ctgacgatgt tggcgctcat gatcatcacc ggcgtcaacg
     1681 actatcggcg cgtgctccgc gagcgggaac ggctggtggg gcaatccgaa gaggacgtgc
     1741 gcatcttcgc cgagaccctg gcactggccg tgagccggaa tgtccggtgg ggacgcacga
     1801 ccgccgagct gaaagagctg ctggacgaca tcctggcgcg gcccgggctg gtcgcggtgg
     1861 ccatctacgg tcccgaagga caggtgatcg cagaaaacgt cgccaccgac gttccgcccc
     1921 cggcagtgga ccgagtcgtg cgcaacacct tgacgaccaa ggagcccgcc ctcggcaccc
     1981 tggagatgca gtcggcacgg gtcgtccggt atgtgcagcc cttccgctgg cccggaggcc
     2041 gcaccggcgc catcgaggtc cgacagacct tggagggaat ggagcgggag ttccagcgtg
     2101 cggtccggga aagcatcctg tcccgcctgg tggtcctgac gctcttcgtc ctgtcgatcg
     2161 tggttctgac ccgttggagc atcggcaggc cgatccgggc cctgatcgcc gccgcgcgtg
     2221 cggtgggacg gggggatctg gatcaacgga tcgacgtgaa gcgtcgggac gagatcgggc
     2281 agttggcgga ggagttcaac cgcatggccg agaatctcca gtcggcgcat gaggagatcc
     2341 tccggcaggg ggaggagcgt ctgcggctgg agggcgaggc ccagcaggcc cagaagctgg
     2401 ccgcggtcgg catgctggcg gccgaggtcg cccatgagat cggcacgccg ttgaacatca
     2461 tctcgggacg ggccgaggtc ttggggaggg ttgtgactcc cgatcagcca gaacaccgaa
     2521 acctggacgt gatcctgctg cagacgcagc gaatcagcaa gatcattcgt gccctcctgg
     2581 actacacccg gccgcgccgc ccgacgttcc ggaccgagga cgtcgtgccg atcatcggcc
     2641 gcgtttccga cctcctcctg gagcgcagcc ggcggcgcgg ggtccgcatc gtgctggatc
     2701 ttcccgtggg gcttcctcag atccaggccg acccggatca gcttcagcag ctcttcctca
     2761 acctgctgct gaatgccctg gatgcatctc cccagggtga gacggttctg gtgacggtgg
     2821 gaccggagcc cgtgctgccg gcggaggatc gggccgggat cgtccggggt cggctcgagg
     2881 gacccagcct ggcgatccat gtcgtcgacg ccgggaaggg gttgaccgcg gagcagctga
     2941 atcacgcctt cgaaccgttc ttttccacca agagccaggg ccagggcacg gggctcggtc
     3001 ttcccatcgt cgagcagatc gtccgggcac atcacggcga gatcgagatg ctgagcattc
     3061 cgggccgggg aaccgaggtg atcctgcgcc tgccgctggt tccggagccg gggacgaccg
     3121 cccccacggc tgcgacgcac cacgcgattc ggaaggcgga tgcaaatgga cagtgatccg
     3181 tacctggagc tgcaagggga tcccgtcctt cacccccgca tcccggtggc gacctaccgg
     3241 ctccagttca atcccagctt caggttcgag accgccggcc gcctggtccc gtacctggag
     3301 gcgctcggga tcagcgacgt ctacgcctca tcgtatctgg ccgcgcgacc cgggagcctg
     3361 cacggctatg acatcgccaa ccacaacgcc ctgaatcccg agatcggaac ggaggaggag
     3421 taccacgggt tcgtcggggc gctgcaagcc gcaggcatgg ggcagatcct ggacgtggtg
     3481 cccaaccaca tggggatcgc cgccgcctgc aatccctggt ggaacgacat cctggagaac
     3541 ggacccggct ctccctacgc cgagttcttc gacatcgatt gggatccggt gaagcgacag
     3601 ctggccaaca aggtcctgct ccccattctg ggtgaccaat acggtcgtgt gctggatggt
     3661 ggagagctcc ggctggagta tgcggaaggg ggcttctcgc tccggtacta cgacacccgg
     3721 ctgcccatcg cgccccgctc ggtgattcgg atcctggggc accgcctgga ggagctgacg
     3781 gccgccttgg gggaagacag cccccacctg caggaatacc tgagcatcct caccgccctg
     3841 accaacctgc ccggccgaac cgaaacctcg cccgagcgcg tgcgtgagcg gaggcgcgag
     3901 aaggaggtga ttcgccgccg cctgacagac ttgattaccg cctgcgagcc ggtccgcacc
     3961 tccatcgagg agaccattcg gatcttcaac ggcaagacgg gcgatccccg gagcttcgat
     4021 ctgctggacc gcctcctgga tgagcaggtc taccggctgg cctactggcg ggtggcggcc
     4081 gaggagatca actaccgtcg cttcttcgac atcaatgatc tggcggccat ccggatggag
     4141 aatccggccg tcttccgggc agcccatcaa ctgatcttcc ggctggtgga ggagggcaag
     4201 gtcaccggtc tccgcctcga ccacccggac gggctgttcg atccgccaca atacttccac
     4261 gccctgcagc gagagcggct gtcccaggtg ctccgagccc gtccgctgtc ggaaggcgct
     4321 gcggatcggc ttgatcggga gacgctggtc gctcggggcg tggcgcagtt cgactccacg
     4381 tgtcagccgg acccgacgca tctgggctgc cgccccctgt acctcctggc ggagaagatc
     4441 ctggcccggg gagaacgtct gccgttgcaa tgggccatcc acggaaccac gggctacgag
     4501 ttcttgaaca tcgcggaggg cctgttcgtt gacagcgaga acgagaaggc catgaccgcc
     4561 gcctacaccg tcttcaccgg ccagcgcaag cctttcgccg acctggtcta ccagtccaag
     4621 cagctcatcc tgcaagtctc catgtcgagc gagctgaacg tcctcgggca caccctcgac
     4681 cggctgtcgg agcggagccg gtactcgcgg gatttcacgc tcaacagcct cacccatgcc
     4741 ctgcaggagg tgatcgcctg cttcccggtg taccggacgt atatcgacgg ccgaggcgcg
     4801 gaggtggtcc tgcaggaccg ggcctgcgtc gaggtggcgg tggccttcgc caagcggcgc
     4861 aaccccacca ccaacgtctc cgtgttcgac ttcgtgcgag atgtgctgct cctgcggtac
     4921 cccgagagtg cagatgcgtc ctaccgccgg gatcaactgg ccttcgtcca gaagttccag
     4981 caggtgaccg cgccggtcac ggccaagggg gtggaggata cggccttcta ccgctacaat
     5041 cgtcttgtga gtttgaacga ggtgggaggg gagccggacc ggttcggcat ctccctggag
     5101 gagttccaca aacagtgcct ggcccgccag gagaagtggc ccaacgggct atctgccggc
     5161 tccacgcacg ataccaagcg tagcgaggat gtccgggccc ggatccacgt cctctcggag
     5221 attcccaggg agtggcgggc ggcggttggt cgttggcacc ggtggaaccg gaggcacctg
     5281 gcggaagtgg atggacgccc tgctcccgac cggaacgatg agtacttgct ctaccagacc
     5341 ttggtcggcg cctggccgct cgcacccatg ggcccggagg aggtctcggc attcacggcc
     5401 cgcatccagg actacctcct gaaggcggcc aaggaggcca agctcaacac gagctggatc
     5461 aatccaaacg aggcctacga tgacgccctc cagaccttcg cggcccgcat cctcgacccg
     5521 tcccctacca atcggttcct cacggacttc accgccttcc atcccttcgt ggcgcggctg
     5581 ggaatggtga acagtctggc ccagacaacg ctcaggatca ccgcgcccgg ggtgcctgat
     5641 ttctatcagg ggagcgagct gtgggacttc agcctggtgg atccggacaa ccggcgccct
     5701 gtcgacttcg ccactcgatc cagccttctc gaggacctgc ggcgccgcat tgcgacgggc
     5761 gatctggtgg gcctggcacg cgaagtggtg gagcagtgga cggatggccg ggtcaagctg
     5821 tacaccatcc accgggccct gatgtaccgg cgaaccgccg ccgacctttt ccagagaggc
     5881 gactacgtgc cgttgccggc gggagggcag gctgcattgc acctgtgcgc cttcgcgcgt
     5941 cgcctggcga ccaggaccgc gatcacggtc gtcccccgtc tcagcgcttc gctgaccgac
     6001 aacggcgccc ggctgcccct ggggcgcgac gtgtggggtg atacctgggt ggcactgccc
     6061 cgcgatctgg cgggcggacc ctatcggaac ctgttcaccg gcgaga
//
LOCUS       DBNGZC010000627         2425 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2206922, whole genome shotgun sequence.
ACCESSION   DBNGZC010000627 DBNGZC010000000
VERSION     DBNGZC010000627.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2206922"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="AC16G0_10890"
     CDS             complement(<1..699)
                     /locus_tag="AC16G0_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100153.1"
                     /translation="MTRGRRIMRHAGGVGWFLSSLILMLLSTSGPAQAGTAGDFSERL
                     DKAIRQVEAAQDRGTPLGDASALFPESEEVTGPEGEMRLDHTSLRTEWNSVAPEGNAR
                     KESLERLRHRLTAVRAEATRTWNAAAEGAPQSLGWREKLTEVLSLPEFRKGQSGEDWR
                     SRFMQWLLDKLAFVFPRGATQAVGSVLRWMIYALAGAALVAVLFVLVRVALPLFARHG
                     GGVRAPTPSAPMPAE"
     gene            complement(696..1811)
                     /locus_tag="AC16G0_10895"
     CDS             complement(696..1811)
                     /locus_tag="AC16G0_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR5100154.1"
                     /translation="MKTPTFDLQPMSTGDILDRTIRLYRRHFLHTLAIVAVPFLLIIP
                     AWAVLGTGFAPRGRVMPWRSPGLMVGGALFGLAYVWLWFVSMGALARSVSEHYLGGTP
                     TIWTAYSPVLRRSFSLIWVYVLIWLVGGAILGAGGGIFAGAMVIVNLLPTTAGYVMAA
                     ALGIAAVVVLAIAVGTFIRSILVTQVIVIEDVRGWAAVKRSWRLMRPCTGKAVVIFLF
                     GIAVSFIISFLFNLPAGVVMALRPGSTTFILGKAWEGLGQILAAPLLMIAFTLLYYDS
                     RIRQEAFDLEMMAQNLGVSKGPATSSQTNTPPRPSISRSPSPAGPLTRPAGSLPRPIA
                     AFKVCPKCGAQVPNIQPSCGKCGTRVPYRPATHSPAP"
     gene            complement(2132..>2425)
                     /locus_tag="AC16G0_10900"
     CDS             complement(2132..>2425)
                     /locus_tag="AC16G0_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HIR5100155.1"
                     /translation="GMALGWPDAQPIVRPRLPTAAVLHWETYDPEPRDAALHEYDRTM
                     IATGIYQGRQVPVPGKAGEMQDYGWLEHSARRVSQALRTDLRLVLKRQGFALE"
BASE COUNT          477 a          813 c          768 g          367 t
ORIGIN      
        1 ctcggcgggc atcggtgcgg agggcgtcgg tgcccgcact cccccgccat ggcgggcaaa
       61 gaggggaagc gctaccctga caaggacgaa caggactgcg acaagcgcag cacctgccag
      121 cgcataaatc atccacctca ggacactgcc gacagcctgc gtcgcgccgc gggggaacac
      181 aaaggcgagc ttgtctagca accactgcat gaaacgactg cgccagtcct ctccggactg
      241 tcccttcctg aattcaggca gactgaggac ctccgtgagc ttctccctcc agccaaggga
      301 ttgcggggcg ccctctgccg cggcgttcca cgtccgggtc gcctccgcgc ggacggcggt
      361 caggcgatgc cgcagacgct ccagcgattc cttgcgcgcg ttcccctcgg gggcgacgct
      421 gttccactcc gtgcgtaggc tcgtgtggtc caggcgcatc tccccctccg gaccggtcac
      481 ttcctcggat tccgggaaaa gcgcactggc atcacccagc ggggtccccc gatcctgcgc
      541 cgcttccacc tggcggatgg ccttgtccag ccgctcggaa aagtcgccag cagttccagc
      601 ctgcgccggt cctgaggtgc tcagcagcat gaggatgaga ctggagagga accagccgac
      661 accgcccgcg tgcctcatga tccggcggcc acgcgtcacg gcgcgggtga atgggtcgcc
      721 ggacgatacg gcacccgggt gccgcatttt ccgcagctgg gctggatgtt gggcacctgg
      781 gcgccgcact tcgggcagac tttgaaggcc gcgatcggcc gcggtagact ccccgccggc
      841 cgcgtcagag gacctgcggg cgatggggag cgggaaatgg agggacgggg cggggtattc
      901 gtctgcgaac tggtagcggg acccttcgag acccccaggt tctgcgccat catctccaga
      961 tcgaaggcct cctgacggat ccggctgtcg taatacagca acgtgaatgc aatcatcaag
     1021 agcggggcgg caaggatctg gccgagcccc tcccacgcct tgcccaggat gaaggtcgtc
     1081 gagccggggc gcaaggccat caccaccccg gccgggaggt tgaacaggaa ggagatgatg
     1141 aaagacacgg cgatcccgaa gaggaagatc accacggcct tgccggtgca cgggcgcatg
     1201 agtctccagc ttcgcttcac ggccgcccac ccccgcacat cctcgatgac aatgacctgg
     1261 gtgaccagga tcgatcggat gaaggtccca acggcaatgg ccagcacgac aacggcagcg
     1321 atgcccagag cggctgccat gacgtagccg gccgtcgtcg ggagcagatt gacgattacc
     1381 atggcgccgg caaagattcc gccccccgca ccaagaatgg cgcctcccac gagccagatc
     1441 aaaacataca cccagatcag ggaaaaactt cgtcgaagga caggggagta cgccgtccaa
     1501 atggtgggag tcccgcccaa gtagtgctcg gagacggagc gggccagggc gcccatgctc
     1561 acaaaccaca gccagacata ggccagcccg aacaatgcgc ccccgaccat gagcccaggg
     1621 ctccgccacg gcatcacacg gcccctgggt gcaaatccgg tcccgagcac cgcccatgcc
     1681 gggatgatca gtaggaaggg cacggcgacg atggccaagg tgtgcagaaa gtgacgacgg
     1741 tagagacgga tcgtccggtc caggatatcc ccggtagaca tgggctggag gtcaaacgta
     1801 ggcgttttca tttccgcctc tggccgggcc cgagacacgg cccgtagggc gcaccgtaac
     1861 gaacaattgg aaacctgtca actccaaccc gtcacttgct cccgatccgc gaccaccgct
     1921 gtgtggtcag tccaaagatc aatcctccgc cccagacttc cgatgaacgc ccagattcta
     1981 caccatcaac ttcagatcga aggactcctt cctgctgcag cggtggcacc atgatcgcgc
     2041 ctgaggcagt cgtctgctcg gcggagatgg caggataccc gctgagctta gagacgctcg
     2101 ccaagccgcc gctccaacgg caagacatgt cttattccag ggcgaatcct tgccgcttca
     2161 ggacgaggcg caggtccgtc ctcagagcct gggaaacccg gcgcgccgag tgctccagcc
     2221 agccgtagtc ctgcatctcg cccgccttgc ccgggaccgg gacttgccgc ccctggtaaa
     2281 tgccggtggc gatcatggtc cggtcgtact cgtggagtgc agcgtcccgg ggctcgggat
     2341 cgtaggtctc ccagtgcagg accgcggcag tcgggaggcg cggccggaca atcggctggg
     2401 cgtcgggcca gcccagcgcc atgcc
//
LOCUS       DBNGZC010000628         3116 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_935753, whole genome shotgun sequence.
ACCESSION   DBNGZC010000628 DBNGZC010000000
VERSION     DBNGZC010000628.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_935753"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..695
                     /locus_tag="AC16G0_10905"
     CDS             141..695
                     /locus_tag="AC16G0_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR5100156.1"
                     /translation="MSESLLRASGQAVAPEAMTPLERIAFRVNWMLERVCAALAAAMV
                     LIIWYGVFERYVIRAGGAWTEELSRYVMIWLALLAVPCAAYYREHIGLELFLARLSLR
                     HRKTLILALDIVSVAFFLFLTYSGILLTQDGTTAYATIFSMNMVIPFASVPVSSALAA
                     FQFFVAMIRMQKQMAAQLASGGAA"
     gene            692..1975
                     /locus_tag="AC16G0_10910"
     CDS             692..1975
                     /locus_tag="AC16G0_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR5100157.1"
                     /translation="MSLVLILFFGLMFVGLPIGVTLGVAGVLGLVQIGGQNFLMMAPR
                     RYFQGLDLFTFMAMPFFILAGEIMNRSGITDRLTAFANAIVGHFRGGLAHSNMIASVI
                     MAGMTGAAVAETAAFGNTMVPAMVKSKYPRPFACAVVVAGSIIGPTIPPSNLMVIYGS
                     LMGTSIAGLFAAGIVPGLLICLFCMIIIGIVGKRINLPKNAEGFSLMGLLWAFKSSIL
                     ALLMPVIILGGILGGVVTPTEAAAIAVAYALVVSTVVYRAIGFSDVLEMLIKTARITG
                     VVFLIIAAASILSWWMTFLRLPQQVASLFLSVSTDRYMVITMILALLLFIGTIMDINA
                     ALIILAPVLGTLTSSIGMDPVHAGIMIVLALNIGLMTPPVGACLFVMCSISGETIERI
                     SRPLLPFIIVETLVLFIVSYWEKLTLFIPRILLGM"
     gene            2095..>3116
                     /locus_tag="AC16G0_10915"
     CDS             2095..>3116
                     /locus_tag="AC16G0_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020886113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR5100158.1"
                     /translation="MRTVWKALILALALALAASPAMAAKIIKLHHLNQDDPFDNPTGA
                     MATVFKSLVEAGTNGSITVQTFPNGQLGKDNEVLEQVKSGVIESGIFSVGGFAGGYPL
                     IGVLDVPFAFPNISATYAVFDGPFGQRMAQDIKKKTGMTVLGFGDSGGFFHISNNKKP
                     ITSPADMVGLKIRTMGLDTHKAIISSLGGQPTALAWAEVYTALQTGVADGQMNPIPII
                     AFAKFQEVQKYLTLTGHLFAPYVWVMNTKFWDGLTRDEKNVVTYSAKSAIVAGRGIAR
                     TIEASNRGLAALSKTMKVNSLTDEQKKAFKDKTTPEVTKIISQKFGAEGTEMMNAFLQ
                     AVKAAE"
BASE COUNT          541 a         1014 c          918 g          643 t
ORIGIN      
        1 atgaccaatg accactttcc aatgaccaag ggtggccgcc gacgtacgct gttgcgcagt
       61 atccggcgaa tggcaggcgc cgctgagatt cggccgtcgg gacctggcta tggctattgc
      121 cggaggaggg tcattcgaat atgtccgaat cgttgctgag agcttcgggg caggctgtcg
      181 cgcccgaggc catgacccca ttggaaagaa tcgccttcag ggtcaactgg atgctggagc
      241 gggtgtgcgc ggcgctggcg gcggccatgg tcttgatcat ctggtacggc gtgtttgagc
      301 gttatgtcat ccgcgccggg ggcgcctgga ccgaggagct ctcccggtac gtcatgatct
      361 ggctggccct gctggccgtg ccctgcgcgg cctactaccg ggagcacatc gggctggaac
      421 tgttcttggc gcggctctcc ctcaggcaca ggaagaccct catcctcgcc ctcgatatcg
      481 tcagcgtcgc ctttttcctg ttcctgacct attccgggat cctcctgact caggacggca
      541 ccacggccta cgccaccatc tttagcatga acatggtcat cccctttgca tcggtgcccg
      601 tctcgtcggc cctggccgcc ttccagttct tcgtggccat gatccggatg caaaagcaga
      661 tggccgcgca actggcgtcg ggaggtgcgg catgagcctg gtcctgatcc tcttcttcgg
      721 tctgatgttc gttggcctgc ccatcggcgt gacgctgggc gtggccggcg tgctcggcct
      781 ggtccagatc ggcggtcaga atttcttgat gatggctccg cggcgatact tccaagggct
      841 cgacctcttc accttcatgg ccatgccctt cttcatcctg gccggggaga tcatgaaccg
      901 ctccggcatc accgaccggc tgacggcctt cgccaacgct atcgtgggtc attttcgagg
      961 tgggcttgcc cactccaaca tgatcgcatc agtcatcatg gctggcatga cgggcgcggc
     1021 tgtcgccgaa acggcggcct tcgggaatac gatggttccg gccatggtca agtccaaata
     1081 tcctcgcccg tttgcctgcg ccgtggtggt cgcgggctcc atcatcggcc ccaccatccc
     1141 gccctccaac ctcatggtca tctacggctc gctcatgggc acctccatcg cggggctctt
     1201 cgcggccggc atcgtgccag gtcttctcat ctgcttgttt tgcatgatca tcattggcat
     1261 cgtggggaag cggatcaacc tgcccaagaa cgccgagggc ttcagtctga tgggcctgct
     1321 gtgggccttc aagtccagca tcctggccct cctgatgccg gtcatcatcc tgggtggcat
     1381 cctgggtggc gtcgtcacgc ccacggaagc ggccgccatt gccgtcgcct atgccctggt
     1441 cgtcagcacg gtggtctacc gcgccattgg cttcagcgat gtcctggaga tgctcatcaa
     1501 gaccgcccgc atcaccgggg tggtcttcct catcatcgcc gccgcctcga tcctgagctg
     1561 gtggatgacc ttcctgcgtc tgccccagca ggtcgcctcg ctgttcctca gcgtctctac
     1621 cgaccggtat atggtcatca ccatgatcct ggccctgctg ctgttcatcg gcacgatcat
     1681 ggacatcaac gccgcgctga tcatcctggc gccggtccta ggcaccttga ccagctccat
     1741 cggcatggac ccggtgcacg ccggcatcat gatcgtcctg gcgctgaaca tcggcctgat
     1801 gactccaccg gtgggtgcgt gcctcttcgt catgtgctcc atctccgggg agaccatcga
     1861 gcggatcagc cgcccgctgc tccccttcat catcgtggag actttggtct tattcatcgt
     1921 gtcatactgg gagaagttga ccctgttcat cccccggatt ctcctgggca tgtgaccggg
     1981 aggagccgcc ggctccgtgc aggcgaagcg gaccgaccgc agaggggacc agtcgcccgt
     2041 cctagatcgt cgttcaacat gggcagtccg attgccgcaa cacaggagga ccacatgcga
     2101 accgtttgga aagcgctcat cctggccctg gctctggccc tggccgcttc tccggccatg
     2161 gccgccaaga tcatcaagct gcaccacctc aaccaagacg acccgttcga caacccgacc
     2221 ggcgccatgg ccaccgtctt caagagcttg gtggaggccg gaaccaacgg cagcatcacc
     2281 gtacagacct tccccaacgg gcagctcggc aaggacaacg aagtcctgga gcaggtgaag
     2341 tcgggtgtca tcgagtccgg tatcttctcc gtgggcgggt tcgccggcgg ctacccgctg
     2401 atcggggtgc tggacgttcc cttcgccttc cccaacatca gcgccaccta cgcagtcttc
     2461 gacggcccct tcggccagcg gatggcccag gacatcaaga agaagaccgg catgacggtg
     2521 cttggcttcg gcgactccgg cggattcttc cacatctcca acaacaagaa gcccatcacg
     2581 tccccggcgg acatggttgg tttgaagatt cgcaccatgg gtctggacac ccacaaggcg
     2641 atcatatcgt ctctgggcgg gcaacctacc gcgctggcct gggccgaggt ctataccgcg
     2701 ttgcagaccg gcgtggccga tggtcagatg aatcccattc cgatcatcgc cttcgccaag
     2761 ttccaggagg tgcagaagta cctcaccctg accgggcatc tcttcgcccc gtacgtgtgg
     2821 gtgatgaaca ccaagttctg ggatggcctc acgcgcgacg agaagaacgt ggttacctat
     2881 tccgccaagt cggccatcgt ggcagggcga ggcatcgccc ggaccatcga agcttccaat
     2941 agggggctgg ctgccctgtc aaagaccatg aaggtcaact ccttgaccga cgagcagaag
     3001 aaggcattca aggacaagac cacacccgag gtcaccaaga tcatcagcca gaagttcggg
     3061 gccgagggta cggagatgat gaatgcgttc ctgcaggcgg tgaaggccgc cgagta
//
LOCUS       DBNGZC010000629         2825 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_55545, whole genome shotgun sequence.
ACCESSION   DBNGZC010000629 DBNGZC010000000
VERSION     DBNGZC010000629.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_55545"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="AC16G0_10920"
     CDS             complement(<1..417)
                     /locus_tag="AC16G0_10920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5100159.1"
                     /translation="MNFDLSDEQRMMRDTVRGFAEKEVKPRAAEIDRTDEFPWDLWKR
                     MADLGLLAMTLPPEYGGSGMDTVAWAIAQEELARASVVVADLQLLSKLMQDVLLRNGS
                     ESQKEKYLAAMGRGEKVCVFGQTEPGTGSDVAGITTT"
     gene            complement(509..1294)
                     /locus_tag="AC16G0_10925"
     CDS             complement(509..1294)
                     /locus_tag="AC16G0_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR5100160.1"
                     /translation="MSDQSTRRFVRWGQEEGVATLIVERPPLNPLSFQTKEEIGACLD
                     EIAADPGVRCLVIHGAGGRAFSVGSDIKEFPEATARRLGHQRAVHEHTLNNRIAYFPV
                     PTIAAIEGHCLGGGLELALACDLRVASEASRLGLPEVKLGGFPGGGGTERLPRLIGEA
                     RARELIYTGAPVDAREAWRIGLVSRVAPPGQALAAAQEMARAIAANAGATLRTVKSVL
                     DRGRDMSLLEAEQVAIAAIAEIFQSAAFQEGVDAFLEKRPPRF"
     gene            complement(1458..2693)
                     /locus_tag="AC16G0_10930"
     CDS             complement(1458..2693)
                     /locus_tag="AC16G0_10930"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR5100161.1"
                     /translation="MSMAYEGIRIIDFTQMEQGAVGTQVLADFGAEVIKIERIETGDL
                     MRGNRPIVNGLSSFFAACNRNKKSLSLDLKTAEGKEIIYRLVKISDVVASNFRPGVME
                     RLGFGWEDLSRINPRIICAYASGYGQTGPYRDRRGQDLVAQAMGGLMALTGERDGPPA
                     SAGTFVADFVGGMLLAQGIMAALAARERTGRGQVVDSCLLNTVVTFSIGEATAHLNSG
                     QKYPRAYRGGCHSAFGPLYAAYQTKDGKWLVIVGTYVDRELERVFTALGVESDLSRDP
                     RFQASAKGYPDIGLGREAELRPILEQAFSRLTREEALRRLEEQDILAAPVYEHSEVFA
                     DPQVIHNEMVQAIEHPVAGHLKLVGVPVKLSETPARLRLAPPTVGQHNEEILGLLGCT
                     REQTRALQEQGIVGSENLK"
BASE COUNT          462 a         1013 c          846 g          504 t
ORIGIN      
        1 ggtcgtcgtg atgcctgcga catcggagcc ggtcccgggc tcggtctggc cgaacacgca
       61 gaccttctcg ccgcgcccca tggcggcgag atacttctcc ttctgactct ccgacccgtt
      121 gcgcagaagc acgtcttgca tgagcttgct caggagctgc aggtcggcca ccaccaccga
      181 tgcccgcgcc agctcctcct gcgcaatggc ccacgccacg gtatccatcc ccgacccgcc
      241 gtactcggga ggcagcgtca tcgccaagag cccgaggtcg gccatccgct tccagagatc
      301 ccacggaaac tcgtcggtcc ggtcgatctc ggccgcccgc ggcttcacct ccttctccgc
      361 gaacccgcga accgtgtccc gcatcatgcg ctgctcgtcg ctgagatcga agttcatcga
      421 atcccccttc tcggtcgccc gcagaggtgt cgacggctcc tggcggccac cgctcggccc
      481 gtcgaggggt ggcccctccc ctggtccttc aaaacctcgg cggtcgcttc tccaggaagg
      541 cgtccacgcc ctcctggaac gccgcggact ggaaaatctc ggcgatcgcg gcaatggcca
      601 cctgctctgc ctccagaagg ctcatgtccc gtccgcggtc gagcaccgac ttcaccgtgc
      661 gaagcgtggc tccagcgttg gcggcaatcg cccgtgccat ctcctgcgcg gcggccaagg
      721 cttgccctgg aggtgccacg cggctcacca ggccgatgcg ccaggcctcg cgcgcatcca
      781 ccggcgcccc ggtgtagatc agctccctgg cccgcgcctc gccgatcagg cggggcaatc
      841 gctccgtccc gcctccgccc gggaatcctc ccagcttcac ctcgggcagc ccgaggcgcg
      901 aagcttcgct ggcgacgcgc aggtcgcagg ccagcgccaa ttccagaccg ccccccagac
      961 aatgtccttc gatggccgcg atggtcggga cgggaaaata ggctatccgg ttgttcaggg
     1021 tgtgctcgtg gacggcgcgc tgatggccca gccggcgggc ggtggcctcg gggaattcct
     1081 tgatgtccga tcccaccgag aaggcccgcc cccccgctcc gtggatgacg aggcagcgga
     1141 ctccgggatc ggcggcaatt tcatccaggc aggccccgat ctcctccttg gtctgaaagg
     1201 acagcgggtt caggggtggc cgctccacga tcagcgtggc gacgccttct tcttgacccc
     1261 accgaacgaa ccgccgcgtc gactgatcgg acatctggct tcctccatgc gtgagcggga
     1321 atccgcagcc tgccgccgag tcggtctctc cgccccctcc gacagccgga accccagccg
     1381 cggacacccg gatgaagggg atgcgcggca gcatgtctca tgtgactcga gacgtggctg
     1441 catcggcagt cgtcttctta cttcaaattt tccgagccga cgatgccctg ctcctgcaac
     1501 gctcgcgtct gctcccgggt gcagcccaac agccccagga tttcttcgtt gtgctggccc
     1561 accgtgggag gcgccagacg cagtctggcc ggcgtctccg acaacttcac cggcacaccg
     1621 accagcttca agtggcctgc aaccgggtgc tcgatcgctt gcaccatctc gttgtgaatg
     1681 acctgaggat cggcgaagac ctccgaatgc tcgtagaccg gcgccgccag gatatcctgc
     1741 tcttccaggc ggcgaagggc ctcctcccga gtcagccggg aaaaagcctg ctccaggatg
     1801 ggacgcagct ccgcctcgcg tcccagaccg atatccgggt acccctttgc gctggcttga
     1861 aatcgaggat cgcgggacag gtcactctca acgccgagtg cggtgaacac ccgctccagt
     1921 tccctgtcga catacgtgcc cacgatcacc agccacttgc cgtccttggt ctggtaggcc
     1981 gcgtagagag ggccaaaggc cgaatgacaa cccccgcggt acgcccgcgg gtacttctgg
     2041 ccggaattga gatgggcggt ggcctctcca atcgaaaagg tcaccaccgt gttgaggagg
     2101 cacgaatcca ccacctgccc tctgccggtc cgctcccggg cggccagggc ggccatgatt
     2161 ccctgggcca agagcatccc tccaacgaag tcggccacga aggtcccggc tgaggcggga
     2221 gggccatcgc gctctccggt caacgccatg aggccgccca tggcctgggc gacgaggtcc
     2281 tggcctcgcc gatcccggta tgggccggtc tggccatacc ccgaggcgta ggcgcagatg
     2341 atgcgagggt tgatccggct caaatcttcc cagccaaagc ccaggcgctc catcaccccg
     2401 gggcggaagt tgctggccac gacatcggaa atcttcacca gcctgtagat gatctccttg
     2461 ccttccgctg ttttcagatc gaggctcagg ctcttcttgt tgcgattgca ggcggcaaag
     2521 aacgagctca acccgttcac gatggggcga ttcccacgca taagatcgcc cgtctcgatg
     2581 cgttcgatct tgatgacctc ggcgccgaaa tcggccagca cctgggtccc caccgccccc
     2641 tgttccatct gggtgaagtc gatgatgcga attccttcgt aagccatgct catgctgcga
     2701 tccctcggtg tctgcggtgc ccccgggcct ccaattcgag cgggcggttg gcagggttgc
     2761 gggggctgtt catcaccctc actgacgctg cccggtgccc ggcatgcaaa tcgggctact
     2821 caatc
//
LOCUS       DBNGZC010000630         7688 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_837341, whole genome shotgun sequence.
ACCESSION   DBNGZC010000630 DBNGZC010000000
VERSION     DBNGZC010000630.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7688)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7688
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_837341"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="AC16G0_10935"
     CDS             complement(<1..1214)
                     /locus_tag="AC16G0_10935"
                     /inference="COORDINATES: protein motif:HMM:NF024634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5100162.1"
                     /translation="MIPHRELFWNIPAHLWLYPLFLPFLVIFLVGCYRLIKLVWMGQP
                     ERDIPPVGRQLRDLVGQAVLQCRLLGQPLAGGMHAAISWGFGILFVATCLVAVQDYLG
                     VPVLQGDFYLYFMSLTVDLFGLAATAGIVIALIRRYARKPDRLWKPRDAEGYGPFLWL
                     LLAILVTGFLVEGLRIGATQDPWRLWSPGGWVAALGLAPLSQAQQALWHRITWWGHAV
                     LAFGFIALLPYTLIRHILAAAANIALRRPQPSGVIQPVALEDAEHFGIARIEGFPRKD
                     LLDLVACTECGRCQDACPAWATGKALTPKGLILDLRDLLLGGRSGNGKAMVGEVVSED
                     VIWACTTCGACQHECPVFIEPIPKIVNMRRFLVMEEARFPETMQAAMRGLENRGHPYQ
                     GVVPSRTEWAKGL"
     gene            complement(1294..2277)
                     /locus_tag="AC16G0_10940"
     CDS             complement(1294..2277)
                     /locus_tag="AC16G0_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR5100163.1"
                     /translation="MAEILVLAEGEAGVPSRSSLELLGAARCVADATGGRVKAVVLEG
                     SPGGLSPSLFAHGADEVLRVEHPLLAVRQADPCVAALAQVAPQAGAQVVLLADDPCGR
                     EVGPRLAHRLKGGVVTEVTGVEANGEALVFHRQVYGGRCIAALCPKRWPVVATVKPRS
                     MEPAQEQANRGGKETTVAVALDPSVVRTRILQTVAEEVRGVKLENARVVVSGGRGLKG
                     PEPFQQLEELAQLLKGAMGASRAATDAGWVPASRQVGQTGKSVSPDLYIAVGISGAIQ
                     HLAGMTGSKTIVAINTDPDAPIFKMAHLGIVGDFKAVLPRFIDKCRELVQS"
     gene            complement(2359..3159)
                     /locus_tag="AC16G0_10945"
     CDS             complement(2359..3159)
                     /locus_tag="AC16G0_10945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR5100164.1"
                     /translation="MNIIVCIKQIIDPEIPGEQFKLDPATRRQIRGGLSLVISAYDQN
                     ALELALQLREKTGGKITALCLGGTDALGAVKSAMGMGVDAGVLVNDPPLLSADAFGVA
                     HVLARAIQKIGMPDLVLTGCVSGDTGYKVTGPLLAEELGVPGLAFVSRIEIRDGRIVA
                     RRVVEDGYELVDAPLPLVASVVSDDSNVPRYSKLKDIMLAAKKQVPVWKAGDLGLDAA
                     AVGAEAVRMEIQDLTIPQRESRCEFMEGETPQERAERLALRLRELKVI"
     gene            complement(3267..4406)
                     /locus_tag="AC16G0_10950"
     CDS             complement(3267..4406)
                     /locus_tag="AC16G0_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR5100165.1"
                     /translation="MEFELTDEQRMFQETARQFAEKELAPIAAQVDELGQFPSESVRK
                     MGELGFMGVCVPPEYAGAGADTVCYALAVEEISRACASHGVIMSVNNSLFCAPVLEYG
                     TEEQKQRFLSPVAGGETMGCFCLSEPASGSDAASLQTTMRRQGDSYVLNGTKNFVTNG
                     NEAGYAVVFATLDRSLGHKGICAVVVEGGTPGFSVTRLEKKLGIRGSSCAQLAFEDCR
                     VPAANLLGREGEGFKIALTSLDGGRIGIASQAVGIARAALEASLEYAKQRVQFGKPIA
                     AQQAIRFMLADMATETDAARLLTLQAAQLKDRHVRHTKESSMAKLYASEVAMRAATKG
                     VQIFGGYGYMLDYPVQRFMRDAKITEIYEGTSEVQRMVIAHALLS"
     gene            complement(4439..5221)
                     /locus_tag="AC16G0_10955"
     CDS             complement(4439..5221)
                     /locus_tag="AC16G0_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR5100166.1"
                     /translation="MDFETIQVSHDGGVTRLTLNRPKQLNALNQQLFRELDQVLDQVA
                     EDPAVRVVVITGAGERAFAAGADIREFEAMGPVEALEFSRNTQRVLRKLETLPKPTIA
                     AVNGFALGGGCELMMACDIAYAADSAKIGQPEITLGIIPGGGGTQRLPRLLGKQKAKE
                     LVLTGEALSAEEAHRLGLVSKVVPAADLMAEVQTLCGKLLSKGAIALRMAKEAIELGS
                     ETDLAAGMEIEAKSWSVCFTTQDRLEGVKAFLEKRKPNFQGN"
     gene            complement(5257..6108)
                     /locus_tag="AC16G0_10960"
     CDS             complement(5257..6108)
                     /locus_tag="AC16G0_10960"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003388468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5100167.1"
                     /translation="MEIRTVGVVGAGTMGHGIAQVALQGGVRVALRDVEERILQQARA
                     RIEKGFDRLMESGKMGETQKADCMKMLSITTNLADLKNVDFIVEAATEDFAVKKTIFQ
                     ELDRICRPSTILATNTSSISITKIGATTRRADRVIGMHFMNPVAILQLVEVIRGVETS
                     AETAATTHDLAKRLGKTAVEAGDFPGFLANRILMPMINEAIFTLMEGVGTVEAIDTVM
                     HLGMKHPMGPLALADLIGLDVCLAVLRVLHEGLGDPKYRPAPLLVRMVDAGHIGRKSG
                     RGFYNYS"
     gene            complement(6292..7470)
                     /locus_tag="AC16G0_10965"
     CDS             complement(6292..7470)
                     /locus_tag="AC16G0_10965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR5100168.1"
                     /translation="MEDVVIVGAARTPIGSFGGRLASVPATRLGSVAAAEALRRAGVD
                     PQHVDETIFGNVLSAGLGQAPARQVAMGAMVPATAGALTVNKVCGSGLMAVMLAANRI
                     QAGEAELVLAGGMESMSRAPYLLDRARTGYRMGHAQMYDSMILDGLWDVYNQFSMGLC
                     GELCADRYHFTREAQDEYAARSYTRALAAQAEGRFSREIVPVEVSGRKGAVEVVDEDE
                     EPKRGDLAKMRTLKPAFKDGGTVTAAISPSVNDGAAALVVVSARRAEALGLLPMARIA
                     GMAGAATAPEWFTVAPIDAVRRLFKKTGWTAENVDLFEINEAFSVVTMAANQDLGLDP
                     ERVNVNGGAVALGHPVGATGARILVTLLHAMANRGAGRGIATLCLGGGEAVALAVERN
                     "
BASE COUNT         1279 a         2763 c         2329 g         1317 t
ORIGIN      
        1 agacccttgg cccactctgt ccggctcggc acgaccccct gatacgggtg gccccggttc
       61 tccaacccgc gcatggctgc ctgcatcgtt tcgggaaagc gcgcctcctc catgaccagg
      121 aagcgccgca tgttgacgat cttggggatc ggctcgatga agaccgggca ctcgtgctgg
      181 caggcgccgc aggtggtgca ggcccagatc acgtcctcgc tcaccacctc acccaccatg
      241 gccttgccgt tcccgctcct tcctcccagc agcaggtcgc ggaggtccag gatcagtccc
      301 ttcggtgtca gggccttccc cgtggcccag gcgggacagg catcctggca ccggccgcat
      361 tccgtgcagg ccacgaggtc cagcagatcc ttgcgcggga agccttcaat ccgggcgatc
      421 ccgaagtgct cggcgtcctc cagggccacc ggctggatga cgcccgaggg ttggggccgg
      481 cgcagggcga tgttcgccgc ggctgccagg atgtgccgga tcagcgtata gggaagcagg
      541 gcgatgaagc cgaaggccag gaccgcgtgc ccccaccacg tgatgcggtg ccagagagcc
      601 tgttgcgcct gcgacagcgg ggcgagaccc agggccgcga cccagccccc cggtgaccac
      661 agacgccagg ggtcctgcgt ggctccgatg cggagcccct ccacgaggaa gcccgtcacc
      721 aggatggcga gcaagagcca gaggaagggg ccgtagcctt ccgcgtcccg cggcttccag
      781 agccggtcgg gcttcctggc gtagcgcctg atcagcgcga tgacgatccc cgccgtcgcc
      841 gccagaccga acaggtccac cgtgagcgac atgaagtaga ggtagaagtc tccctgcagg
      901 acgggaaccc ccaggtagtc ctgcacggcc acgaggcagg tggcgacgaa caggatgccg
      961 aacccccagg agatggcggc gtgcatcccg cccgccagcg gctggcccag caggcggcac
     1021 tgcaacaccg cctgcccgac gaggtcgcga agctgacggc cgaccggggg gatgtcccgt
     1081 tccggctgcc ccatccacac cagcttgatc aggcggtagc agccaaccag gaagatgacc
     1141 aggaacggca agaagagggg atacagccag aggtgcgctg gaatgttcca gaagagttcc
     1201 cgatggggga tcattcacgg gactccgaaa ggtcgcgggg catgcgacgc gggcttcggg
     1261 ctgtcacgac actccagcca tcttgggtcg ccgtcagctc tgcaccagct cccggcactt
     1321 gtcgatgaac ctcggcagca ccgccttgaa gtcgcccacg atgccgaggt gcgccatctt
     1381 gaagatcggc gcgtcgggat cggtgttgat cgccacgatg gtcttggagc cggtcatgcc
     1441 cgccaggtgc tggatcgcgc ccgagatccc cacggcgatg tagagatcgg ggctcacgct
     1501 cttgccggtc tgccccacct gccgagaggc gggcacccac ccggcatcgg tggcggcccg
     1561 cgaggccccc atggccccct tgagaagctg cgccagctcc tccagttgct ggaacggctc
     1621 ggggcctttg aggccgcggc ctccgctcac gaccacacga gcgttctcca gcttcacccc
     1681 gcggacctcc tcggcgaccg tctgcaggat cctggtccga acgacggagg ggtccagggc
     1741 gacggcgacc gtggtctcct tcccgccgcg attggcctgc tcctgcgccg gttccatgct
     1801 ccgcggcttg acggtcgcga ccacaggcca tcgctttggg cagagggccg cgatgcagcg
     1861 cccaccatac acctgccggt ggaagaccag cgcctcgcca ttcgcctcca cccccgtgac
     1921 ctcggtgacc accccgccct tcaggcggtg ggccaggcgg gggcccactt ccctgccaca
     1981 cgggtcgtca gccagcagga cgacctgcgc ccctgcctga ggcgcaacct gggcgagcgc
     2041 cgccacgcaa ggatcggcct gccgcacggc gagcagcgga tgttccaccc gcaagacttc
     2101 atccgctccg tgggcgaaca gggatggaga caggccgccg ggcgagccct ccagaaccac
     2161 cgccttcacc cgtccgccgg tcgcatcggc gacgcaacgg gccgccccca gaagctccag
     2221 gctgcttcgc gaggggaccc ctgcctctcc ttctgccagc acaagaatct ctgccatcga
     2281 tgcctctcct ccggcgggtg atcggcgctc ggcgtcgttg ccggcctaga gcaaacggct
     2341 gaccgcgctg ggctgttctt agatgacttt gagctcgcgc aggcggaggg cgaggcgttc
     2401 ggctcgctcc tgcggcgtct cgccctccat gaactcgcac ctggactcgc gctgggggat
     2461 cgtgagatcc tggatttcca tcctgaccgc ctcggctccc accgctgcgg cgtccagacc
     2521 caggtcaccc gccttccaca cgggcacctg cttcttggcc gccagcatga tgtccttgag
     2581 cttggaatag cggggcacgt tgctgtcgtc gctcacgacg ctggccacca ggggcagcgg
     2641 cgcgtccacc agttcgtacc cgtcctccac cacccggcgg gccacgatcc ggccgtcccg
     2701 aatctcgatc cggctcacga aggccagacc gggcaccccc agctcctcgg ccaggagcgg
     2761 gccggtcacc ttgtacccgg tgtcgcccga cacgcacccc gtgagaacca gatccggcat
     2821 cccgatcttc tggatcgccc tcgccaagac gtgcgccacc ccgaaggcat cagcgctgag
     2881 gagcgggggg tcgttcacca ggaccccggc atccaccccc atgcccatgg cgctcttcac
     2941 cgcccccaga gcatccgtcc cgcccaggca cagggccgtg atcttccccc cggttttttc
     3001 gcgaagctgc agcgccagct ccagagcatt ctggtcgtag gcgctgatga ccagggagag
     3061 gccgccccgg atctgccgcc tggtcgccgg gtccagcttg aactgctcgc ccgggatctc
     3121 ggggtcgatg atctgcttga tacagacgat gatgttcatg cgttgagcct cagaattgag
     3181 gaaagagcgt ccggcggttc gggtgttcgg cagttctgcc gagatttgca cggccgaacc
     3241 gccgaacagt cgaacggccg aacgttctat gatagcagcg cgtgcgcgat gaccatccgc
     3301 tgcacctcgg aggtgccctc gtagatctcg gtgatcttgg cgtcccgcat gaatcgctgg
     3361 accggatagt ccagcatata cccatacccg ccaaagatct gaaccccctt ggtggcggcg
     3421 cgcatggcga cctcggaggc atagagcttg gccatggacg attccttggt gtggcggacg
     3481 tggcgatcct tcaactgggc cgcctgcagc gtgagcaggc gagccgcgtc ggtctccgtg
     3541 gccatgtccg ccagcatgaa ccggatggcc tgctgggccg cgatgggctt cccgaactgc
     3601 acccgctgct ttgcgtactc cagcgaagcc tccagggcgg cccgcgcgat cccgacggcc
     3661 tgggaagcga tgccgatgcg ccccccgtcg agcgaggtca gggcaatctt gaatccctca
     3721 ccctcccggc ccagaaggtt cgccgccggc acgcggcagt cctcaaatgc caactgagcg
     3781 caactgctgc cccggatgcc cagcttcttc tccaggcggg tcacagagaa gcccggcgtt
     3841 cccccctcca cgacgacggc gcagatcccc ttgtgcccca gcgagcggtc gagcgtcgcg
     3901 aagaccacgg cgtaccccgc ttcattgccg ttggtcacaa agttcttggt gccgttgagg
     3961 acgtaggagt ccccctggcg tcgcatggtc gtctgaaggc tggcggcgtc gctccccgag
     4021 gcgggctcgc tcaggcagaa acagcccatg gtctccccgc ccgccacggg cgagaggaag
     4081 cgctgcttct gttcctcggt cccgtattcg aggacgggag cgcagaacaa ggaattattc
     4141 acggacatga tgacgccatg ggacgcgcag gcgcggctga tctcctccac cgccagggcg
     4201 tagcacaccg tgtccgcacc cgcgcctgcg tactcggggg ggacgcacac gcccatgaag
     4261 cccaactccc ccatcttccg gacgctctcg ctggggaact ggcccagttc gtccacctga
     4321 gccgcgatcg gcgccagctc tttctcggca aactgccggg ccgtctcctg aaacatccgc
     4381 tgttcgtcgg tcagctcgaa ctccatagag cccgccatcc gccatacact gtgagccgtc
     4441 agtttccctg aaaattgggc ttgcgtttct ccaggaaggc cttcacccct tcgagtcggt
     4501 cctgcgtggt gaagcagacg ctccaggact ttgcctcgat ctccatcccg gccgcgagat
     4561 ccgtctccga gcccagctcg atggcctcct tcgccatgcg gagggcgatc gcccccttgc
     4621 tcagcagctt gccgcagagc gtctgcacct cggccatgag gtccgccgcc ggcaccacct
     4681 tggagaccag acccagccgg tgcgcctcct cggcggataa cgcctcgccg gtcaacacca
     4741 actccttggc cttctgcttc cccagcagcc gagggagccg ttgcgtcccg ccgccgccgg
     4801 ggatgattcc cagggtgatc tcaggctgcc cgatcttggc cgagtcggcg gcataggcaa
     4861 tgtcgcaagc catcatcagc tcgcagcctc cgccgagggc gaatccgttg accgccgcga
     4921 tcgtcggctt gggcagcgtc tccagcttcc gcaggacgcg ctgggtgttc cggctgaact
     4981 ccagcgcttc cacagggccc atggcctcga actcgcgaat gtccgcccct gcggcaaagg
     5041 cacgctcgcc cgccccggtg atgaccacga cccgcactgc ggggtcctcc gccacctgat
     5101 cgaggacctg gtccagctcc cgaaacagtt gctggttcag cgcattcaat tgcttcgggc
     5161 ggttcagcgt gagtctggtc accccgccgt cgtgggagac ctggatggtt tcgaagtcca
     5221 tggcccctcc catgcgccct cgtggggaca ggcagatcag gaatagttgt aaaatcctcg
     5281 gccgctcttt ctcccgatat ggccggcgtc caccatcctg accagcaagg gcgccggacg
     5341 gtacttggga tcacccagcc cttcgtgaag cacccgcagc acggccaggc aaacgtccag
     5401 tccgatgaga tctgccaggg ccagcgggcc catggggtgc ttcatcccca ggtgcatgac
     5461 ggtgtcgatg gcctccaccg tccccacccc ttccatgagg gtaaagatgg cctcgttgat
     5521 catcggcatg agtatccggt tggccaggaa gccgggaaag tctccggcct ccacggcggt
     5581 cttccccagt cgcttggcca ggtcgtgagt cgtggccgcc gtctccgcgg atgtctccac
     5641 gccgcggatg acctccacca actgcaggat ggcaaccggg ttcatgaagt gcatcccgat
     5701 caccctgtcg gcccgccggg tggtcgcccc gatctttgtg atggaaatag aggaggtgtt
     5761 ggtggccagg atggtggagg ggcggcagat cctgtccaat tcttggaaga tcgtcttctt
     5821 caccgcgaaa tcttcggtgg cggcttcgac gatgaaatcg acgttcttca gatccgccag
     5881 gtttgtcgtt atggacaaca tcttcatgca gtcggccttc tgcgtctccc ccatcttccc
     5941 ggattccatc agcctgtcga accccttctc aatccgggcg cgggcctgct gcaggatccg
     6001 ctcttccacg tcgcgcaggg cgacgcgcac cccgccctgg agagccacct gtgcgatgcc
     6061 atgacccatg gtaccagctc ccaccacccc gacggttctg atctccatgg cgcctcctca
     6121 agacgtaaga aagcctgaca gggaaatcca acagcgatag ccaatcggga gaggaaaatc
     6181 cgataaatgc ggttccgtgc actccctcgg gagtccgaca gcaaatcctc tagcgatagc
     6241 ttgacgtcag aatgtgctcg ctgtcgctgt ccctgtcgcg gtcatccgtt gtcaattccg
     6301 ttcgaccgcc agggcaacgg cctcccctcc gcccaagcat agggtagcga tgccgcgacc
     6361 agccccgcga ttcgccatgg cgtggagaag ggtcaccagg atccgggccc cggtcgcccc
     6421 cacggggtgg cccagggcca cggccccgcc gttcacgttc acgcgctcag gatccagccc
     6481 gaggtcctgg ttggcagcca tggtcacgac cgagaacgcc tcgttgatct cgaacagatc
     6541 cacgttctcc gcggtccaac ccgtcttctt gaacagccgg cgcaccgcgt caatgggggc
     6601 cacggtgaac cactccggcg cggttgcagc cccggccatc ccggcgatcc gcgccatcgg
     6661 cagaagaccc agagcctcgg ctcgccgcgc cgagaccacg accaaagccg cggccccatc
     6721 gttcaccgag ggggagatgg ccgcggtcac ggtcccgcca tccttgaagg ccggcttcag
     6781 agtccgcatc ttcgccaggt ctccgcgctt cggctcctcg tcctcgtcca cgacctcgac
     6841 cgcgcccttc cgaccagaga cctccaccgg gacgatctca cgcgaaaatc ggccctctgc
     6901 ctgggcggcc agggcgcggg tgtagctcct agccgcatac tcatcctgcg cctcgcgcgt
     6961 gaaatgatac ctgtcggcgc acaactcgcc gcataatccc atggagaact ggttgtacac
     7021 gtcccacagc ccatccagga tcatggagtc atacatctgg gcatgaccca tgcggtagcc
     7081 ggttcgagct cggtccagga ggtacggggc ccggctcatg ctttccattc ccccggccag
     7141 caccagctcc gcctcccccg cctgaatgcg attggctgcc agcatgaccg ccatcagccc
     7201 tgagccgcag actttgttca ccgtgagagc ccctgcggtg gccgggacca tcgcccccat
     7261 ggcgacctgc cgcgccggcg cctggccaag ccccgccgac aggacgttcc cgaagatggt
     7321 ttcgtccacg tgttgcgggt ccacaccggc gcgtcgaagg gcctcggcgg ccgccacgct
     7381 gccgagtcgc gtcgccggca ccgacgctag tctaccgccg aaacttccga tgggggttcg
     7441 agcagccccc acgatcacca catcttccat gacctttccc ccgctcttca gctcccgccc
     7501 tgacctaagt tggtaaagtc cgttgcgtct cttgagcccc gaagcctttg actgcccggc
     7561 cctcctaccc tcgccttgcc tcagctcctc accgacccgt tgacggcaat cccgtccaag
     7621 atcaggtcgg tcaggctctc gatgacgtta tccagcgtgt agcctttcag gttccaccgt
     7681 ttcagatt
//
LOCUS       DBNGZC010000631         1747 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_737896, whole genome shotgun sequence.
ACCESSION   DBNGZC010000631 DBNGZC010000000
VERSION     DBNGZC010000631.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_737896"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="AC16G0_10970"
     CDS             <1..443
                     /locus_tag="AC16G0_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HIR5100169.1"
                     /translation="KVRHLLHMRDLQEETGGFMCFVPLAFDPSGTELAHLPVTTGYAN
                     LREIAVARLLLDNFPHVKAFWIMITPAVAQMALWYGADDIDGTVSHYEITHALGTASH
                     RQALSQAELLDLIREAGRVPVERDALYNLVEQTEPAGIGQAPGD"
     gene            676..>1747
                     /gene="mqnC"
                     /locus_tag="AC16G0_10975"
     CDS             676..>1747
                     /gene="mqnC"
                     /locus_tag="AC16G0_10975"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HIR5100170.1"
                     /translation="MTERASSLDDIETKILAGERLSLEEGVHLYREASPLQLSSWADL
                     VRRRLHPEGTVSYVIGRNINYTNVCWVKCKFCTFYRLPGQEGGYILPQAEIFQKIQAL
                     VDLGGTEILIQGGLTPALKIEYCEELFRAIKARFPVHVHGLSPVEIIYLAKISRLSER
                     ECLRRLKAAGLDSVPGAGAEMLVDEVREQIAPLKGSAAAWLDLMRTAHQLGIPSTVTM
                     MYGSVETVEQRLEHLIKVRELQDETGGFRAFIPWSFQPSGTHLAEVKRASGLDYLRTV
                     AVSRLMLDNIPNIQASWLTQGPKIGQLALQYGVNDMGSTVLEENVVTARGAIFMVPLA
                     EIERLIRDAGFRPARRNTRYEIL"
BASE COUNT          304 a          579 c          586 g          278 t
ORIGIN      
        1 agaaggtccg gcacctcttg cacatgcggg acctgcagga ggagacgggc gggttcatgt
       61 gcttcgtccc gcttgccttc gacccctccg gcaccgagct ggcccatctg cccgtgacca
      121 ccggctacgc caacctgcgg gagatcgcgg tggcccgcct actcctggac aatttccccc
      181 acgtcaaggc cttctggatc atgatcaccc cggcggtcgc ccagatggcc ctctggtacg
      241 gggcggatga catcgacggc acggtctcgc actacgagat cacccatgcc ctggggacgg
      301 cctcgcaccg ccaggccctg agccaggccg agctcctcga cctgatccgc gaggccggcc
      361 gcgtgccggt ggagcgggat gccctctaca acctggtcga gcagacggag ccggccggca
      421 tcggccaggc cccgggagac tgaccagatc ccaaggatta accccaatcc gaaattcctg
      481 aaccgccaag gcgccaagag cgccaagatt cacagggctc agaatgcaga cgttctgacg
      541 gacgccaccg taccatgggg tgctcttgag aagtcggccc tcacatcagc tcttggcggc
      601 cttggcggcc ccggtggcgt cttggcggtg aagcggtttc ctcggatcag ggttgactgt
      661 caatcgcgag gtccgatgac ggaaagagcg agttccctgg acgacatcga gaccaaaatc
      721 ctggcggggg agcgcctgag cctcgaggag ggggtgcacc tgtaccgcga ggccagcccg
      781 ctgcaactct cgtcctgggc ggacctggtg cggcggcggc tccatcccga agggacggtg
      841 agctacgtca tcgggcgaaa catcaactac acgaacgtct gctgggtgaa gtgcaagttc
      901 tgcacgttct accggctgcc gggacaggag ggcggctaca tcctgccgca ggcggagatc
      961 ttccagaaga tccaggcgct cgtggatctg ggcgggaccg agatcctgat ccagggcggg
     1021 ctgactccgg ccctcaagat cgagtactgc gaggagctgt tccgcgccat caaggcccgc
     1081 ttcccggtgc acgtgcacgg gctctcgccg gtcgagatca tctacctggc caagatctcc
     1141 cgcctgagcg agcgcgaatg cctgcgtcgc ctcaaggccg ccggcctgga ctcggtcccg
     1201 ggggcggggg cggagatgct ggtggacgag gtgcgggagc agatcgcgcc gctcaagggc
     1261 tcggcggcgg cgtggctgga cctgatgcgg acggcccacc agctgggtat cccgtccacg
     1321 gtgaccatga tgtacggatc ggtggagacg gtggagcagc ggctcgagca cctgatcaag
     1381 gtgcgcgagc tgcaggacga gacggggggc ttccgggcct tcatcccctg gagcttccag
     1441 ccgtcgggga cgcacctcgc cgaggtcaag cgcgcgagcg gtctggatta cctgcggacc
     1501 gtggccgttt cgcgcctgat gctggacaat atcccgaaca tccaggcctc gtggctcacc
     1561 caggggccga agatcggcca gctggctctc cagtacggcg tcaacgacat gggcagcacc
     1621 gtgctcgagg agaacgtggt cacggcccgg ggcgccatct tcatggtgcc cctcgccgag
     1681 atcgagcgcc tgatccgcga cgccggcttc cgccctgccc gccgcaacac ccggtacgag
     1741 atcctta
//
LOCUS       DBNGZC010000632         3298 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1424997, whole genome shotgun sequence.
ACCESSION   DBNGZC010000632 DBNGZC010000000
VERSION     DBNGZC010000632.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1424997"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1928)
                     /locus_tag="AC16G0_10980"
     CDS             complement(39..1928)
                     /locus_tag="AC16G0_10980"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR5100171.1"
                     /translation="MEGPFNKKFLHVDLTAGTTSVEEVPTLTYRMYLGGAAMLAYLLN
                     RELKPGVDPLGPENVLVFAAGVGNGSPLSGSTRFSVGAKSPLSGGYGESEAGGWWGPE
                     LKLAGFDAVIVKGASPRPLYLWIDDGQVELRDAAHLWGKISGDVQDILKGETDKRARV
                     LQCGIAGENGVLFANVVNELKHFNGRCGLGAVMGSKRLKAIVVRGTNKIEAKNPEAMA
                     ALLKWHRETYDRNTDMLHKLGTPRTVRGLNKDGILPTHNFRRGQFEQADEISGERMKE
                     TILADEGTCFACAVACKRDVEVPELGVTSRYGGPEYETLGAAGSGCGIGDLKMVALFN
                     QICAQHVMDTISTGVSISFAMECFENGLITAKDTDGLDLRFGNAEAMLAMTEKIARRQ
                     GFGAVLADGVRRAAKRIGNGAERFAMQVKGQEIPLHEPRGKQSMSLAYTMAPGGADHC
                     RHPHDPAYEGLHPDGAHALEPLGLCEPLKRLDLSQRKVRAFYYATNWNIMMSSTGMCQ
                     LAVSPANIINPTRVTDLVRALTGWDTSLWELMKVGERGKALARVFNCREGISPKDEVL
                     PARFHEAFTDGPLQGVRVDPETFSKARRLYYQMEGWDPETGWPTFAKLAELGIEWAAK
                     SGDCW"
     gene            complement(2114..>3298)
                     /locus_tag="AC16G0_10985"
     CDS             complement(2114..>3298)
                     /locus_tag="AC16G0_10985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR5100172.1"
                     /translation="TIPFSTGAMQIFFGIEQLTYLAIPLLILSGTLMHVAGIAERIVD
                     FAQVLVGRMRGGLGIADIVASLIFADMSGSAVSDTAAIGSLVIPEMKKRGYRAEFATA
                     LQASAGTLGLMCPPTITLLVYATVIGVSVSRLFAAAILPAVLVAVSFAVVTYLHARRH
                     GYPAERVARGEILPRIWRALPGLFAAVVVVGGILGGIFTPAEAGVVLLVYVLLVGVTL
                     YRKSVGARQIIRTTIQAGYTSGMTLCLISTSVAVGFLLASDLIPMHIAQYITQLTKDR
                     HMVLLILNLFFIATGILLEPPAVIVAFLPAVMPLLHNVGVDPVLWGVIFVINAGIGMI
                     HPPVGLTLYVSAAIGGVRIERAALAALPFLGIMLVDLALVSLWPNIALLLPHLLFGYP
                     LQ"
BASE COUNT          619 a         1071 c         1044 g          564 t
ORIGIN      
        1 gaagagaatc atccgcagtt cgcgagttga cctcccaatt accagcagtc ccccgacttc
       61 gccgcccact cgatccccag ctcggctagc ttagcgaagg tgggccagcc ggtctccggg
      121 tcccagccct ccatctgata gtacaatcgg cgcgccttgc tgaaggtttc cgggtcaacc
      181 cggacaccct gtagcgggcc gtcggtgaag gcctcgtgga acctggccgg gagcacctcg
      241 tccttgggcg agatgccctc ccggcagttg aagacgcggg ccagggcctt gccacgctct
      301 ccgaccttca tgagctccca gaggctggta tcccacccgg tcagggcccg gaccagatcg
      361 gtcacgcggg tggggttgat gatgttcgca ggagacacgg ccagctggca catccctgtc
      421 gagctcatca tgatgttcca gttggtcgcg taatagaagg ctcgcacctt gcgctgggac
      481 aggtccaggc gcttcagggg ctcgcacagg ccaaggggct ccagggcatg ggccccgtcg
      541 ggatgcaggc cctcgtatgc cgggtcgtga ggatggcggc agtggtcggc cccacccggg
      601 gccatggtgt aggccaggga catcgactgc ttaccccgcg gctcgtgaag cgggatctcc
      661 tggcccttga cctgcatggc aaaccgctcg gcgccgtttc cgatccgctt tgcggcgcgc
      721 cgcaccccgt cggccagaac cgcgccaaag ccctgacgcc gcgcgatctt ctccgtcatg
      781 gcgagcatgg cttcggcgtt gccgaatcgc aggtcgagac cgtcggtgtc cttcgccgtg
      841 atcaggccgt tctcgaaaca ctccatggcg aaggagatgc tcacgcccgt cgagatcgta
      901 tccatcacgt gctgcgcgca gatctggttg aacagcgcga ccatcttgag gtctccgatg
      961 ccgcagccgg agccggcggc gccgagcgtc tcgtactcgg gcccgccata ccgggacgtc
     1021 acgcccagct ccggcacctc cacatcgcgc ttgcaggcga cggcgcaggc gaagcaggtt
     1081 ccctcgtcgg cgaggatggt ctccttcatg cgctcgccgc tgatctcgtc ggcctgctcg
     1141 aattgtcccc gccggaagtt gtgggtcggc aagatgccgt ccttgttcag tccgcggacg
     1201 gtcctgggcg tcccgagctt gtgcagcatg tcggtattcc ggtcataggt ctcgcggtgc
     1261 catttgagga gcgccgccat ggcctcggga ttcttggcct cgatcttgtt cgtgccccgg
     1321 acgacgatgg ccttgagacg cttgctcccc atcaccgccc cgagcccgca gcgcccgttg
     1381 aagtgcttca gttcgttcac cacgttggcg aacagcaccc cgttctctcc agcgatcccg
     1441 cactggagga cgcgcgcccg cttgtccgtt tcacccttca ggatgtcctg aacatctccc
     1501 gagatcttgc cccacaggtg tgcggcatcc cgcaattcca cctggccgtc atcgatccat
     1561 aaatagagcg gccggggcga ggcccccttg acgatgacgg catcgaaccc ggcgagcttg
     1621 agttccggtc cccaccaacc gcccgcctca gactcaccat accccccgct gaggggggac
     1681 ttggcaccca cgctgaagcg ggtcgacccg gagaggggcg atccgttccc aacgccggcg
     1741 gcaaacacca ggacattctc gggccccaac ggatccacgc ccggcttgag ctcccggttg
     1801 agcaggtagg ccagcatggc cgcgccgccg agatacatgc gataggtgag ggtgggcact
     1861 tcctccacgc tggtcgtgcc cgcggtcaga tcgacgtgca ggaatttttt gttgaatggc
     1921 ccttccatgc tcattccttt cttccaggtg gttgtattga taccggctga tcagtgatga
     1981 aaaagccaca cctgcaacca aatcttcgtg gcacccccta tccccaagcc tttcatctcc
     2041 tccgaactgt gcaagagcgg ccagtcccag gcacgggcat tgcgaggatc tcccagatgc
     2101 cattcctctt gcgctattgc aggggatagc cgaagaggag atgggggagg agcagggcga
     2161 tgttcggcca gagcgatacc aaagccagat cgaccagcat gatgccgagg aagggcaggg
     2221 cagccagcgc agcccgctcg atccgcaccc caccgatagc cgccgagacg taaagcgtga
     2281 ggccgaccgg cgggtggatc atgccgatgc cggcattgat cacgaagatg accccccaca
     2341 gcacgggatc gacgcccaca ttgtgcagca acggcatcac cgcgggcaag aaggcgacga
     2401 tgaccgccgg tggttccagc agtatcccgg tggcgatgaa gaacaggttc aggatcagaa
     2461 gcaccatgtg cctgtccttg gtgagctgag tgatgtattg ggcgatgtgc atggggatga
     2521 gatcgctcgc cagcaagaaa cctacggcga ccgacgttga gatgaggcag agagtcatgc
     2581 cactggtata gcctgcctgg atggtcgtcc ggatgatctg ccgggcgccg acgctcttgc
     2641 ggtagagagt gacgcccacc aacagcacgt aaaccagcag cacgacaccg gcttccgctg
     2701 gagtgaagat gcctcccaga atgccaccca ccacgacgac cgcggcgaac agcccgggca
     2761 gtgccctcca gatgcgaggc aggatttccc cgcgggccac gcgctcggcg gggtacccgt
     2821 ggcggcgagc atgcagatac gtcaccacgg cgaagctgac ggccaccagg acagcgggca
     2881 gaatcgcggc ggcgaacagg cggctcaccg agacaccgat gaccgttgca tagacaagca
     2941 gggtgatggt cggcggacac atgaggccga gggtgccggc ggaggcctgg agggcagtgg
     3001 caaactcggc gcggtagccc cgcttcttca tctccgggat caccagcgag ccaatggcgg
     3061 ctgtgtccga cacggccgag ccggacatgt cggcgaagat caggctggcg acaatatcgg
     3121 ctattccaag accgccacgc atccgcccga ccaagacctg agcgaagtcc acgatccgct
     3181 cggcgatgcc ggcgacgtgc atgagggtgc cggagagaat gagcagcggg atagccagat
     3241 aggtgagctg ttcgatgccg aagaagatct gcatggcccc ggtcgagaag gggatggt
//
LOCUS       DBNGZC010000633         2093 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1522721, whole genome shotgun sequence.
ACCESSION   DBNGZC010000633 DBNGZC010000000
VERSION     DBNGZC010000633.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1522721"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(366..>2093)
                     /locus_tag="AC16G0_10990"
     CDS             complement(366..>2093)
                     /locus_tag="AC16G0_10990"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043758 - acetate-CoA ligase (ADP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HIR5100173.1"
                     /translation="NPSVEPDAVVGPGAVALVSQSGATAYGPLMAVAKDRGIGFRYVV
                     STGNEADLDLTDFVEFLLDDPGVKSIALMVEGLRDGPKFVRVARLALRCGKPLVILKL
                     GRSAVGAGAALTHTGALTGSDAVQTAMFRQLGVVRVDDYDELLEVAAMFAKTRAPEGD
                     RVGLISHGGGICGAIGDKCGELGLSVPPLAERTLKTLQTILEGRGAAANPADITYHGG
                     RDTFPVILKTMLEDDNIDLLGVATAGKEKTARAVIDTAKESRKPVLFMWTESVRDTLA
                     LPILQQSEVPVFYLPGRFANGIRSLVEYRRARQRYREEAPTETGPGLATQAMGNAAKA
                     ELTELLQSSAGRVLDEHGSKAALSLLGIPVTREVVCTSLRQAESAAADLGYPVALKVA
                     SPEIPHKTEAGMIRLDVRNERELAEAWDAVLANARAFAPAAAIDRVLVQEMVTGSVEA
                     IVGLSRDAVFGPVLLFGIGGLYVEALGAVSRRLCPITPADAREMIAEVRGMERILGGY
                     RGRPAADIDALVDVLCRLSCMALSAGDLLGEVDINPLAVLPKGLGVKALDALVVAGGT
                     ANVTEGMGN"
BASE COUNT          372 a          760 c          616 g          345 t
ORIGIN      
        1 gcagccggcc gtgctgcgtc cgccatggaa ccctcctcga aattccccga ctttccgctg
       61 ccgcattccc ggatcggctc tccagagtgg caaggctatc cggcgagccg tacacccgcg
      121 tcaagcggac gcacgcggaa acatggagtt gaccgagacg atacccggct cgatggcgcg
      181 ccaagcctcg gctcacgatc gaacgaccgc agggaagact tattccactt gtcggccctc
      241 tgaccatgac gaggacccct gcagagttcg gggtgggcat gttccgcttc ccgacgaccg
      301 cacctccggg acccggacca gcctcttccc ggaagtcgct gatcaatacg gactgcgtgg
      361 tttcctcagt ttcccatccc ctcggtcacg tttgccgtcc cgccggccac caccagcgca
      421 tccagcgcct tgacgcccag ccccttgggg agaaccgcca gcggattgat gtccacctcc
      481 ccaagcaggt cgccagccga gagcgccatg caggagagac ggcaaagcac atcgaccagt
      541 gcgtcgatgt ctgcagccgg gcgaccgcgg tagccgccca gaatccgctc catgcctcgg
      601 acttccgcga tcatctcgcg ggcgtccgca ggggtgattg ggcagaggcg gcgggagacg
      661 gctcccagcg cctccacgta gaggcccccg atcccgaaca agaggacagg gccaaatacg
      721 gcatcccggg acagaccgac gatcgcctcc acgcttccgg tgaccatctc ctgcaccagc
      781 acacgatcga ttgccgcggc aggcgcaaag gcgcgagcgt tcgccagcac cgcgtcccac
      841 gcttcggcaa gctctctctc attccgcacg tccagccgta tcatgcctgc ttcggtcttg
      901 tggggaattt ccggtgaggc gaccttcagc gcgacgggat agcccaggtc ggcggcggcg
      961 gattccgcct gacggagact cgtgcacaca acctcacgtg tcaccggtat tcccaggagg
     1021 gagagagccg ccttgctccc gtgctcatcc agcacgcggc ccgccgatga ttgcaggagc
     1081 tccgtcaact cggcctttgc ggcgttcccc atggcctgcg tcgccaagcc gggcccggtc
     1141 tccgtggggg cctcctcccg ataccgctgg cgggcgcgcc gatactcgac caatgaccgg
     1201 atcccgttgg cgaaccggcc tggcagatag aacacgggga cctcgctctg ctgcaatatc
     1261 gggagggcca gcgtgtcccg aacactttcg gtccacatga agagcacagg cttgcggctc
     1321 tccttcgccg tgtcaatcac ggcccgagcc gtcttctctt tgccggccgt cgccaccccc
     1381 aagagatcaa tattgtcatc ctccagcatg gtcttcagga tgaccggaaa ggtgtctcga
     1441 cccccatggt acgtgatgtc ggcgggattc gcggccgccc ctcgcccctc caggatcgtc
     1501 tgcagcgtct tcagggtgcg ttcggccagg ggaggcaccg acagccccaa ctcgccgcat
     1561 ttatccccga tggcgccgca gattccaccc ccatgcgaga tcaggccgac acggtctccc
     1621 tcgggagcgc gagtctttgc gaacatcgcg gcgacttcca ggagttcgtc atagtcgtcc
     1681 acgcgcacca cgccaagctg tcggaacatc gccgtctgaa ccgcatcgga ccccgtcagc
     1741 gcgccggtgt gggtgagtgc cgccccagcc cccaccgccg atcgtccgag cttgaggatc
     1801 accagcggct tgccgcagcg gagcgccagg cgcgcgaccc gcacaaactt gggaccatcc
     1861 cgtagaccct cgaccatcaa ggcgatggac ttgacccctg ggtcgtccag caggaactcc
     1921 acaaaatccg tgaggtccag atccgcctcg tttcccgtag acaccacata ccggaacccg
     1981 atgccccgat ctttggcgac ggccatgaga ggcccatatg cggtcgcccc gctctgagac
     2041 accagggcca cggcgcccgg gccgacgacg gcatcgggct ccacgctggg gtt
//
LOCUS       DBNGZC010000634         2313 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_641520, whole genome shotgun sequence.
ACCESSION   DBNGZC010000634 DBNGZC010000000
VERSION     DBNGZC010000634.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_641520"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="AC16G0_10995"
     CDS             <1..704
                     /locus_tag="AC16G0_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013954315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HIR5100174.1"
                     /translation="VLAEATRILKALGVTLNVEKTRIVHVTTGFEFLGYKIKRGRRPL
                     KLAPGKIRTGRRHGDLYAYPRQKSIEHFKEQIRQRTRRKSPVSTRELVAELNPVIRGW
                     GQYFCKAHIRKLFAQLDRWILRRIWSQRFKRWRCRGWKRLPERQLYGELGLVRLIYLI
                     PSLVAVHQCSNTSLCEVCAYHYTRQWATISSPRRAPRLSDPSRRISPRSRFGSCHRHA
                     AFRTSRSHRTLHLSA"
     gene            complement(732..1406)
                     /locus_tag="AC16G0_11000"
     CDS             complement(732..1406)
                     /locus_tag="AC16G0_11000"
                     /inference="COORDINATES: protein motif:HMM:NF015038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HIR5100175.1"
                     /translation="MLPRLYTVFKIDPTRSADVLIAVLGTEFDGVLGCDYFSAYRRYQ
                     REFGVTLQFCLAHLIREVKFLTTLPDVRDRTYGERLREALRRLFGVIHRREELPASVF
                     GSQPEAARAEVLRCGTQDVPETKPSRNLAKRLETHGESYFRFLTTPGMEPTNNLAEQA
                     IRFVVLDRLVTQGTRSEVGNRWCERIWTVIATCVQQGRSVFAYLGAAVGAWFTGTETP
                     SLLPEG"
     gene            complement(1346..2158)
                     /locus_tag="AC16G0_11005"
     CDS             complement(1346..2158)
                     /locus_tag="AC16G0_11005"
                     /inference="COORDINATES: protein motif:HMM:NF040425.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6444 domain-containing protein"
                     /protein_id="HIR5100176.1"
                     /translation="MRGEDPADCPNCRRLRARVDAPEAELAALKEIVARLSQQLAAAR
                     KDSTNSSKPPSSDIVKPPKPSPPQGQDRRRIGGQPGHPKHERAAFPPEAINGGSFDHR
                     IDSCPACGHDLQPTLTIAPRIVQQVEIVAAPLSIQEHRNHPGWCPHCQKLYEAPLPPG
                     IQRGGLVGPTLTTLIAYLKGACHASFSTIRKFLRDVVRVTISRGELARIIAKVSRVLE
                     RPYEELLEGLPTQARLNVDEAGHKQNGRRMWTWCFRACTPCSRSTRPAARTC"
BASE COUNT          388 a          801 c          715 g          409 t
ORIGIN      
        1 gggtgctggc cgaggccacc aggatcctga aggcgctggg cgtgacgctc aacgtcgaga
       61 agacgcggat cgtgcacgtc acgaccggct tcgagtttct ggggtacaag atcaagcgcg
      121 gacgccggcc cctgaagctg gccccgggca agatccgtac aggccgccgt cacggggacc
      181 tctacgcgta cccgcggcag aagtcgatcg agcacttcaa ggagcaaatc cgccagcgga
      241 cccggcggaa gtccccggtg agtacccggg agctggtcgc cgaactgaat ccggtcatcc
      301 gcggctgggg ccagtatttc tgcaaggccc acatccgcaa gctcttcgcc cagctcgacc
      361 gatggatctt gcggcgcatc tggtcgcaac gattcaagag gtggcgctgt cggggctgga
      421 agcggcttcc cgagcgtcag ctgtacggcg agttgggact cgtgcgcctg atctacctga
      481 ttccttcatt ggtagccgtt caccagtgca gtaacacttc tctgtgtgag gtgtgcgcgt
      541 accactacac tcgccaatgg gcaaccatca gctctcctcg tcgcgccccc cgcctttccg
      601 acccttcgcg cagaatcagt ccccgaagcc gttttggttc ttgtcaccgg catgctgcct
      661 tcaggacgtc ccggtcccat cggaccttgc acctgagcgc atgactggtt cgctcggcct
      721 cttcgttctc ctcacccctc gggcaacagc gacggcgtct cggttccagt gaaccaggca
      781 ccgaccgccg ctcccaggta cgcgaacacc gaacgaccct gctgaacaca cgtcgcgatc
      841 accgtccaga tccgttcgca ccagcgattc cccacctcgc tccgcgtccc ttgcgtgacc
      901 agccggtcga gcaccacgaa ccggatcgcc tgctccgcca ggttgttcgt cggctccatc
      961 ccgggcgtcg tcaggaaccg gaagtagctc tccccatgcg tctccagccg tttcgccagg
     1021 ttgcggctgg gcttcgtctc cgggacgtcc tgcgtcccac accgcaacac ctcggcacgc
     1081 gctgcctccg gttggcttcc gaacactgaa gcgggaagtt cttctcgccg gtggatcaca
     1141 ccgaacagcc gccgcaacgc ctcccgcaac cgctccccat aggtacgatc ccgtacgtcg
     1201 ggcagcgtcg tcaggaactt cacctcgcgg atcagatgcg ccaggcagaa ctggagcgtc
     1261 acgccgaact cacgctgata acgccggtac gcggagaagt agtcgcaacc caagacgccg
     1321 tcgaactccg tacccagcac cgcgatcagc acgtccgcgc tgcgggtcgg gtcgatcttg
     1381 aacacggtgt acaggcgcgg aagcaccagg tccacatcct ccggccgttc tgcttgtgcc
     1441 cggcctcatc gacgttcagc cgcgcctgcg tcgggaggcc ctccagtaat tcctcatacg
     1501 gccgctccag cacccggctg accttggcga tgatccgggc caactccccc cgcgagatgg
     1561 tcacccggac gacgtcgcgg aggaacttgc ggatggtcga gaaggaggcg tgacaagccc
     1621 ccttgaggta ggcgatcagc gtggtcaggg tcggcccgac caggccgcca cgttggatcc
     1681 ccggcggaag cggggcctcg tacagcttct gacagtgggg gcaccacccc ggatgattgc
     1741 ggtgctcctg gatcgacagc ggtgccgcga cgatctcgac ctgctggacg atccgcggcg
     1801 cgatggtcag cgtgggctgc aggtcgtgcc cgcacgcggg gcaggagtcg atccgatgat
     1861 cgaaggaccc gccgttgatc gcttcgggcg ggaaggcggc gcgttcgtgt ttgggatggc
     1921 cgggctgccc gccgatgcgg cgtcgatcct ggccctgagg cggtgacggc ttgggcggct
     1981 tgacgatgtc ggacgacggg ggcttggagg agttcgtaga gtccttgcgc gccgcggcca
     2041 gttgctggct caaacgggcg acgatctcct tgagggcggc gagttccgcc tccggggcat
     2101 cgactcgcgc ccggagccgt cgacaattgg ggcagtcagc cgggtcctcg cctctcatgg
     2161 cactcgatca tacacacacg acagaacagg cactacaccg gtgaacggcc acttcagcgc
     2221 cgggccggtc cgtcttgcgg cctggttgac cggcatagat tccctgcaat ttcgcctcct
     2281 ccttccgcag tcgttcgccc accacccact cca
//
LOCUS       DBNGZC010000635         9446 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2498525, whole genome shotgun sequence.
ACCESSION   DBNGZC010000635 DBNGZC010000000
VERSION     DBNGZC010000635.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 9446)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9446)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9446
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2498525"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="AC16G0_11010"
     CDS             <1..1196
                     /locus_tag="AC16G0_11010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR5100177.1"
                     /translation="YRRLFEDDLTADYVVAPEGKIHACNPAFVATFGFLDRNEALKAS
                     MVSLYPDAQQHAKFVDLLRQERKIENYECMRRRRDGTLIHVVENAVGGFNPQGELQHI
                     RGYLYDNTAHKLAEQGLREAELRYRNLIEFLPDAVLVADAETILFANPAAATLVGATR
                     PDELVGRRLLDFFHPDSHPAMNARTHQALQARVPGPPEHRKIVRLDSQTLDVETAVTP
                     ITFDGKPCVLRVNRDITARVRAEEALRQKDREISLQLQKIEKLNAALTTLLEHREQEA
                     QHQLESIRATLDKLILPYLENLQATRLDEDQRILVEVMAANLANVASSFARQLETWKT
                     KLTPTEIQVADLLRLGKRTKEVATLLKVSPSAVAFHRNNIRAKLGLTRKPTNLVSYLR
                     VMSQR"
     regulatory      2033..2100
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            2190..3425
                     /locus_tag="AC16G0_11015"
     CDS             2190..3425
                     /locus_tag="AC16G0_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR5100178.1"
                     /translation="MENPYGIATLWFGLALLATFLASRLKVSGALMEILVGIGAAAVI
                     GRLFGLDAMGTNLPWLKFLAGIGAVILTFLAGAELEPAVMRRKWREVTVVGLFGFLGP
                     FLGCTAIARFLLGWSPQASWLGGIALSTTSMAVVYAVMLETGFNRTDYGKGILGACFV
                     NDLGTVIALGLMFAPFTWRTLVFAAVSILAFGLLPLVTPLLVDRYANRTAAFRTKWVL
                     FILFGLGALAVWAGSEAVLPAYIAGMVLATVLGSDHAFMRRLRTLTIGFLTPLYFLRA
                     GSFVSLPALAAAPLVFLALFAGKVGTKIFGLHPVVGAFRREPRERWYYTLMMYTGLTF
                     GTIASLYGLTHGIVSQVQYSHLVAVVIGSAVVPTMIASTVFLPHHLLPRTTGGEEPAR
                     FGMLDPVGHAMLEEPIERG"
     gene            3698..4684
                     /locus_tag="AC16G0_11020"
     CDS             3698..4684
                     /locus_tag="AC16G0_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR5100179.1"
                     /translation="MKYTRILIGLVVVGLIAGASVSAHAAGKYALLKTIDLPGDKGGH
                     GDWTTFDPDTRTVWLSQSPDHNVVVIDAATNTIKAVIPDIKDGNGIALSPQFAFLSDN
                     GSNVTIVVDKRTFKRVATLTPVGKGPNGTNVVPSTGNVYISSDSNDMTVFQATAPFAQ
                     VATFKLQPNPAKDGPDVALYVPSKDRLYQPDDNMIAVIDPHTNKVLAVWDPGIKGQAK
                     PIVYDAKTNHFVLGTTDLKMLVLDADSGKVLASIPVKGKVDETVIDEGLRRAYVGDKA
                     GVIEVIDLDTNAVVGALPSEQNVHTLAVDPTSHAVYVYRNESNKVDVFAPGG"
     gene            4935..5213
                     /locus_tag="AC16G0_11025"
     CDS             4935..5213
                     /locus_tag="AC16G0_11025"
                     /inference="COORDINATES: protein motif:HMM:NF012570.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HIR5100180.1"
                     /translation="MTTQLGGEAVSDALYLVGALCRRYQLSALEKFLESCRSFAEEET
                     LNIAILGRFKAGKSSFLNHLLGQPVLPIGVIPVTTVVTEIQHGPKEQA"
     gene            5298..6599
                     /locus_tag="AC16G0_11030"
     CDS             5298..6599
                     /locus_tag="AC16G0_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR5100181.1"
                     /translation="MKRVQRVRVVLPAMERYPGLCFVDTPGLESVLAHNTDAALEWLP
                     NVGLAVVAVGVDPPLSQHDLELIRALSRYTPHIALLLTKVDVLTEAERVEVAAFVRQQ
                     LERDGGGGVRVFPYSVRSGFEECRRTFETELLAPLKAEAGARRTGILRHKLDALLREC
                     ADYLTVALKAAEATDAERDRLRRQILGSESALEDTRLALRLIVRHTSGTLRASFEARL
                     RTEESPARDRLLAAMNEEFPAWCQSLRLATERFDSWLGAGLSREMAELSQRHRSEFVE
                     PVRRVAGQLTRSLQDFRNRLSERTLEALGIPLRTTELELAAEEPRAPDVRVGRIFDHH
                     WELLSVLIPMGLVRGLLHRHFARKVWDAVFVNLSRLVSQWERSVTTALTRLEREAVLR
                     LDGLVATIETLLASAGREAPQIRTDLQRLEALWNQVSRREG"
     gene            7239..7757
                     /locus_tag="AC16G0_11035"
     CDS             7239..7757
                     /locus_tag="AC16G0_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100182.1"
                     /translation="MGPGPQPCELRWERPIEWDGTGAVEIVCMAAGGRRLAIKGGDHS
                     EMLATCGACTIPRETERRPCLFLVPFKTEREGRPRDYFACRWFYTLKPEDPWSTTGIM
                     CPGCIYWFPRPPVQLFRDLEPMTQDVIRYHQDAWAGRLPPSPFAHWFTPRANPSLPRW
                     RRLLHRVVSLVW"
     gene            7797..8537
                     /locus_tag="AC16G0_11040"
     CDS             7797..8537
                     /locus_tag="AC16G0_11040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR5100183.1"
                     /translation="MKLLVVSDIHANWPALQAITEGADAVVCLGDLVSYGPYPRECLA
                     WVRARAAHVVRGNHDTALADRGDPKAAGFKRELARVTLARHQRSLSRADVAWLRGLPT
                     EAHFRFDEYAFHAVHASPEDHLFSYHLTPELPDEELKNETAKVRADLLLVGHTHLPMI
                     RGAWTKVVVNPGSVGQPLDGDPRASYAVIQDGVAEIRRVAYDVEATVAGIRNMRLAAK
                     SAVALIAMLRTGKPVAGPRSEAEGDASG"
     gene            8553..8972
                     /locus_tag="AC16G0_11045"
     CDS             8553..8972
                     /locus_tag="AC16G0_11045"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5100184.1"
                     /translation="MKRKRPTVHDLNTLGVHFYSVEAYDLAIVQLEQAVALAPESAGI
                     HFNLGGAYYGKGRVADAEREFRHALALDPDHVRAHWFRGLCLERLGQLDAALEEFTCV
                     RRYSTETREGRSAGEEIEAIGVLLRRNGDGESPTDMS"
     gene            8969..>9446
                     /locus_tag="AC16G0_11050"
     CDS             8969..>9446
                     /locus_tag="AC16G0_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100185.1"
                     /translation="MSTIPVAPPRVDPAAPPTLEILPGAGTAGLESREKAKPARRRRR
                     WGQEDLSPWRAEILTRLKKPACLLCGEEAEDLRRYYFWFMNEQYANIPTMERLQRARG
                     FCLRHTRHLLTIGGPSRISFVGEYVLRAGADWLCAVQKGTTPGPPESRTGRHRKTEG"
BASE COUNT         1676 a         2957 c         3124 g         1689 t
ORIGIN      
        1 ggtaccgtcg cctctttgaa gacgacctga cggccgatta cgtcgttgct cctgagggca
       61 agatccacgc ctgcaatccc gccttcgtcg ccaccttcgg cttcctggat cggaacgagg
      121 ccctgaaggc cagcatggtg tctctgtatc ccgatgccca gcagcacgcc aagtttgtcg
      181 acttactccg gcaggagcgg aagatcgaga actatgagtg catgcggcga cggcgagacg
      241 gcaccctcat tcatgtggtt gagaatgcgg tcggcgggtt caatccgcag ggggagctgc
      301 agcatatccg cggctacctg tacgacaaca ccgcccacaa gctagccgag caggggctcc
      361 gagaggcaga gttacgctat cgcaacctaa ttgagttcct cccggatgcc gtcctcgtgg
      421 ccgacgccga gacgatcctc ttcgccaatc ccgccgccgc gacactcgta ggcgccacac
      481 ggcccgacga gctcgtgggc cgccgtctcc tcgacttctt ccacccggac agccatcccg
      541 cgatgaatgc gcgcacccat caggcgctcc aggcgcgcgt cccgggtcca ccggaacacc
      601 ggaagatcgt gcgcctggac tcccagaccc tcgatgtcga gacggcggtg acgcccatca
      661 cattcgacgg gaagccatgc gtcctccggg tcaaccgcga catcacggcc cgggtccgag
      721 cggaagaggc gctccgtcag aaggatcgcg aaatttccct gcagctccag aagatcgaga
      781 agctcaatgc ggccctgacc acgctcctgg agcaccggga acaggaggcc cagcaccagc
      841 ttgagagcat ccgggcgacc ctggacaagc tgatcctccc ctacctagag aacctgcaag
      901 ccacccggct ggacgaagac cagcggatcc tcgtcgaagt catggcggcc aacctcgcca
      961 acgtcgcttc gtcgttcgcc cgccagctcg aaacgtggaa gacgaagctc acccccaccg
     1021 agatccaggt ggcggacttg ctccgcctgg ggaagcgcac caaagaggtg gcgaccctgc
     1081 tcaaggtgtc gcccagcgcc gtcgctttcc accggaataa catccgggcg aagctcgggc
     1141 tgacccggaa acccacgaac ctggtgtcct acctccgcgt gatgagccag cggtagaatc
     1201 ccttcaatgc gtcgaagcca ttgggtgaga ggctttcgca tctcttgagg ggcgctgaag
     1261 agggcggaaa cggtggggag gtctgagaca cgaggtcaag gaggcggaga gcaaccgggg
     1321 aagtgcttca tttgacctcc ccagccctgg tgcggcactt cgaggacctg gtggaaaagc
     1381 tccagagtgg agcgacgggg cagattcggg aagcgatcag ggcatccctg gaaaagatcc
     1441 tcgtggtcga ggatggctcg ttggcgctta cggccaaggc ggatggtctt cgggggcgca
     1501 gacaaccatt ggacattcag ggtgccgggg gagcggaccg acaatggggc gaaccagtca
     1561 ttccgttacc ggcaggcatt ggagggtgat cggcattggt caggctgtcg aggggatgga
     1621 tcgccagttg agcagaacgg tctcctcaac gagcgttcga ttcccccggg ggacaccacg
     1681 caagtatacc gaagaatcct ccgccatctc gacctgcgtg tcccttttgt gggcagggta
     1741 ggtcttgcag cttgagcgtc ctctgccgtt cttgtcggga acaaattgcc cgctgagttc
     1801 caccgcttgc ctgacctgac ggccaggtgc tacgagtggc tctgagcaag gtcaaagatg
     1861 gagttgaccg ttctgactcg ctggggcagg taggatcgct aggccagtgg gccaagggtg
     1921 attcgcgccg caggacctgt tctctacaac acgcgacgaa gggcaccgcc ggtccggcgt
     1981 ccttccagaa agtagttgac cagcggagac taatcgggta catattttag gtatcaagcc
     2041 tggcggtgga gtccgccgag aaccgccacc cgcgatgtgg ccgatgactc ctacggggtt
     2101 cgcttcccgt gggagttttt ttgtgccatc aggaggcagc ccccggacca tcttcgtgcg
     2161 cgggggaccc gcgccaggag gctcacctga tggagaatcc gtacggtatc gctaccctgt
     2221 ggtttggact tgcgctcctc gccaccttcc tcgcctcccg cctgaaagtc tccggcgccc
     2281 tgatggagat tctcgtcggc atcggcgcgg cggcggtcat cggccgtctg ttcggcctcg
     2341 acgccatggg aacgaacctc ccctggctga agttcctggc cggcatcggc gccgtcatcc
     2401 tcacctttct tgcgggggcg gagctggagc cggcggtcat gcggcgcaag tggcgcgagg
     2461 tgaccgtcgt cggcctcttc ggtttcctgg ggcccttcct gggctgcacg gcgattgcgc
     2521 gattcctgct gggctggtcg ccgcaggcta gctggctggg cgggatcgcg ctctcgacca
     2581 cctcgatggc ggtggtctat gccgtcatgc tggagacggg attcaaccgg accgattatg
     2641 gcaagggcat cctgggggcc tgtttcgtga atgacctggg gacggtcatc gccctcgggc
     2701 tcatgttcgc gcctttcacc tggcgtaccc tggtcttcgc ggcggtgagc atcctggcct
     2761 ttgggctgct gccgctggtc acgccgctcc tggtagaccg ctatgcgaac cgcacggcgg
     2821 cattccgcac gaagtgggtg ttgttcattc tcttcggctt gggcgcgctc gccgtgtggg
     2881 cgggcagcga ggctgtgctg ccggcgtata tcgcgggcat ggtgctcgcc accgtcctgg
     2941 ggagcgacca tgcgttcatg cggcggctgc ggacgctgac gatcgggttc ctgaccccgc
     3001 tctacttcct acgggctggc tccttcgtct ccctgccggc cctggcggcg gcccccctcg
     3061 tcttcctcgc cctgttcgcc gggaaggtcg gcaccaagat cttcggtctt cacccggtgg
     3121 tgggcgcctt ccgccgggag ccccgggagc gttggtacta cacgctgatg atgtacacgg
     3181 gcctgacctt tgggaccatc gcgtcgcttt acgggctcac ccacgggatc gtctcccagg
     3241 tgcagtattc ccacctggtc gcggtggtca tcgggagtgc cgtggtgccg accatgatcg
     3301 ccagcaccgt gttcctgccg caccatctgc tcccccggac gacgggggga gaggagcctg
     3361 cgagattcgg aatgctcgat ccggtcggcc atgcgatgct cgaggagccg atcgagaggg
     3421 ggtgacgtag aggataccca cccctgtgag gagtcgatcg ccgattagga gcggccccag
     3481 cgggctccgg gagaagccgc agtccccgtg ccccgaatgg ggggggtggg ccataatttg
     3541 gcttgtccta agacggttcg caggcaatac tggagttgcg atggagttcg cgtatccccg
     3601 agtcaagcgg ggtgataact cctactggcc ttgccggtag gggttttttt gttggggtgt
     3661 gtgggttcgt caagatcgca ggaaagggag gggagcgatg aaatacacac ggatcctcat
     3721 cgggctcgtc gtagtcgggc tcatcgcagg ggcttccgtc agtgcccacg ctgccgggaa
     3781 gtacgcgctc ctgaagacca ttgacctgcc gggagacaag ggaggacacg gggattggac
     3841 cacgttcgac ccggacacac gaacggtctg gctctcccag tcgcctgatc acaacgtggt
     3901 tgtgatcgac gccgccacaa acaccatcaa ggccgtgatt cctgacatca aggatgggaa
     3961 tgggatcgcg cttagcccgc agtttgcctt cctgtcggac aacggcagca acgtcacgat
     4021 cgtcgtggac aagcgcacct tcaagcgggt cgccacactc accccggtgg ggaaagggcc
     4081 gaatggcacg aacgtcgtcc cctccacggg gaacgtctat atcagctcgg acagcaacga
     4141 catgacggtc ttccaggcga cggcgccctt tgcccaagtc gcgaccttca agctccagcc
     4201 caatccggcc aaggatggac cggatgtagc gctctacgtc ccgtccaagg accgtctcta
     4261 tcagccggat gacaacatga tcgccgtgat cgacccccac accaacaagg tgctcgccgt
     4321 gtgggatccc ggcatcaagg ggcaggcgaa acccatcgtc tacgacgcga agaccaacca
     4381 cttcgtcctc gggaccacgg acctcaagat gctggtgctg gacgcggact cgggtaaggt
     4441 gctcgcgtcc atccccgtga aggggaaggt ggatgagacg gtcatcgacg agggactgcg
     4501 ccgcgcctac gtcggcgaca aggcgggcgt gatcgaggtg atcgaccttg acaccaatgc
     4561 ggtggttggc gcgctcccct ccgagcagaa cgttcacacg ctggcagtgg atcctaccag
     4621 ccatgccgtc tacgtgtacc gcaacgaaag caataaggtg gatgtgttcg cgccgggcgg
     4681 ctaggggcga tcgagaaagg gctggtgcaa ggaatcgctt gacggtggcc tgcaggcatg
     4741 cgatatacaa cctaacctca tagcgatgga gttcgcgaat ccctgcttca ggcagggtga
     4801 taactcctac tggaatcgcc agtaggagtt atttttttgg gacaaggact cccccctttc
     4861 gctatgaagg atggatcccg cgggggctcc cttggggatg agcccacgga cctctgtttg
     4921 aaggtggact gcggatgact acacagctag gcggagaggc ggtgagcgac gcgttgtatt
     4981 tggtgggcgc cctttgccgg cgctaccagc tctcggccct ggagaaattc ctggagagct
     5041 gccgaagctt cgccgaggag gagaccctca atatcgccat cctcggccgc ttcaaggccg
     5101 gcaagagcag cttcctgaat cacctcttgg gtcagcccgt gctgccgatc ggggtgattc
     5161 cggtgactac cgttgtgacc gagattcagc acggaccgaa ggagcaggcg tagatcggct
     5221 tccgggacgg gcgaacggaa ttcatcccca tccatcggat caaagaattc atcgccgagg
     5281 ccgagaaccc gggcaacgtg aagcgagtcc aacgggtgcg cgtggtgctc cccgcaatgg
     5341 agcggtatcc gggcctctgc ttcgtggata cccccggact ggagagcgtt ctcgcccaca
     5401 acactgacgc cgctctggaa tggctcccca acgtgggcct tgccgtagta gctgtcggcg
     5461 tggacccgcc cctgtcgcag cacgacttgg aattgatccg cgccctcagc cggtacaccc
     5521 cccacatcgc gctcctgctc acgaaagtgg atgtcctgac cgaggcggag cgcgtcgagg
     5581 tcgcggcctt cgtgcgccag cagctcgagc gggacggtgg cggcggggtc cgggtgttcc
     5641 cgtactcagt ccgatccgga ttcgaggagt gtcggcggac cttcgagaca gagctcctag
     5701 ccccgctcaa ggccgaagcc ggcgcgcggc ggacaggcat cttgcggcac aagctcgatg
     5761 cgctgctgcg cgaatgcgca gattacctga ctgtggccct caaggccgcc gaggccaccg
     5821 acgccgagcg agatcgcctg cggcggcaga tcctgggatc cgagagcgcg ctggaggata
     5881 cgcgcctcgc cttgcgactg atcgtccggc atacctctgg cactctccgg gcgagctttg
     5941 aggcccgatt gcggacggag gagtcccccg cgcgcgaccg tctgctggcc gccatgaatg
     6001 aagaatttcc cgcttggtgc cagagcctga ggctggcgac ggagcgtttc gactcgtggc
     6061 tcggcgccgg cctcagccgg gagatggctg agctctccca gaggcatcgg agtgaattcg
     6121 tggagcccgt gcgccgggtg gctggccagc tcacccgctc actccaagat tttcgcaatc
     6181 gcttgtcgga acggacgttg gaggcgctgg gcatcccgtt gcggacaacc gagttggagt
     6241 tggccgcgga ggagccgcgc gcaccggatg tgcgggtggg ccggattttc gatcaccact
     6301 gggagctatt gtcggttctg atccccatgg gactcgttcg cggcctgctg caccgtcatt
     6361 ttgcgcgcaa agtctgggac gcggttttcg tgaacctctc gcgactggtc tcgcagtggg
     6421 agcgcagcgt cacgaccgcc ctgacccgtc tggaacggga ggcggtactg cggttggacg
     6481 ggctggtcgc cactatcgag accctcctgg cctcggccgg tcgagaggcc ccgcagatcc
     6541 gcacggatct gcaacgcctg gaggcactct ggaaccaggt gtctcgcagg gaggggtaac
     6601 ctcggtggga agtcacgtgg gtgcctttgg tgaggtggtc cgggacgtgc gagcgatacg
     6661 ctggtcgccc cgcggaccat ccagcaaatt catcgcctac ttcaatctgc tggatgcgtt
     6721 tcgggtggcc ggatgccctg tctgcaccct gctggagcag aaggaactga gggcagtggg
     6781 cgcgctcctg tgcgagcagg tgaatgaccg cttcacccgg gatcgcctgg taggatccca
     6841 cgggttctgt aactggcacg cgtggatgct gcctggcgta accaatagtg cgtcaggggt
     6901 ggcgctgatc taccatcacc tcctgggacc acgggctgga accctactcc agggtcgttc
     6961 cggaccgcga ggtcgcgagc atgatccccg cggcgcgcga cgggaagttc gacctcctgg
     7021 gagcgggaat cgtcgggcat tccaatctct ttggtcgggt gatgggagca cgatccagaa
     7081 cttgagccgt ttggcgccat ccagtgtgct catcgtgaac tagggacagg atccctcggg
     7141 ccggtgcgat gctggaagat cctcgggcac tcagaaccaa gacttcgaga accgacgcgc
     7201 caccgcggga agcgtggcga cccgagccac tgccgctcgt ggggccgggg ccgcagccct
     7261 gtgagctgcg gtgggagcgg cccatcgagt gggatggcac cggcgcagtg gaaatcgtct
     7321 gcatggccgc tggagggagg cgcctggcga tcaagggggg cgatcacagc gagatgctcg
     7381 ctacctgtgg cgcgtgcacg atccccaggg agacggaacg gcggccctgc ctctttctcg
     7441 tcccgttcaa gacggagcgg gagggccgac ctcgcgacta cttcgcctgc cgatggttct
     7501 atacgctgaa gccggaagac ccctggagca ccactggcat aatgtgcccc ggctgcatct
     7561 actggttccc gcgtccgccg gtccaactgt ttagggatct cgagccgatg acgcaggacg
     7621 tcatccggta ccaccaggac gcctgggcgg gacgcctccc cccgtcgccc ttcgcgcatt
     7681 ggttcacccc gagagcgaac ccatcgctcc cgcgatggag acgcctcctg catcgcgtcg
     7741 tgagcctggt ctggtaggag tcggaacgtg catccctcgc tggagaatcg caggccatga
     7801 agctcctggt cgtctctgac atccacgcga actggccggc gctccaggcg atcacggagg
     7861 gggccgacgc cgtcgtctgc ctcggggacc tcgtgtcgta cgggccgtat ccccgcgagt
     7921 gcctggcctg ggtgcgggcg cgcgccgccc acgtcgtccg cggcaatcac gacacggcct
     7981 tggcagatcg aggggatccg aaggcggcgg gcttcaaacg agaactggct cgggtgaccc
     8041 tggcgcgcca ccaacgcagt ctctcgcgag ccgatgtagc gtggctgcgc gggctgccca
     8101 cggaggcgca cttccgcttc gatgagtacg cattccacgc ggtgcatgcc tccccggagg
     8161 atcatctctt cagctaccac ctcacgccgg agctccccga cgaggagttg aagaatgaga
     8221 cggcgaaggt ccgggcagat ctcctcctcg tcggtcacac gcacctcccc atgatccggg
     8281 gcgcttggac caaagtggtg gtgaaccccg gaagcgtcgg gcagcccctg gacggagacc
     8341 cgcgggcgag ctatgccgtg atccaggacg gggtggcgga gattcgccgc gtcgcctacg
     8401 atgtcgaagc cacggttgcc gggatccgga acatgcggct ggccgccaaa tcggccgtgg
     8461 ccttgatcgc aatgctccgg accgggaagc cagtcgctgg tcccaggtcc gaggccgaag
     8521 gggacgcgtc cggctgaacc gtaggaggtt ggatgaagcg gaagcgaccg acggtccacg
     8581 acctcaacac gctgggcgtc cacttctaca gtgtcgaggc ttacgacttg gccatcgtgc
     8641 aactggagca ggcggtggcc ctggccccgg agtcggccgg catccacttt aatctcggcg
     8701 gcgcatacta cgggaagggt cgcgtcgccg atgccgaacg cgaattccgc cacgcgcttg
     8761 cgctcgaccc ggatcatgtg cgcgcgcact ggttccgggg gctctgcctc gaaaggctgg
     8821 ggcagctcga cgcagcgttg gaggagttta cgtgtgtccg ccgatattcc acggagaccc
     8881 gggaaggtcg ctctgcaggc gaggagatcg aagcaatcgg ggtcctgctc cgccgaaatg
     8941 gcgacggaga atcgcccacg gatatgtcat gagcaccatt cctgtggcgc cgcctcgggt
     9001 agaccccgcc gcacccccga ccctggagat cttgcccggc gcaggaaccg ccggcctcga
     9061 atcccgcgag aaagcgaagc cggctcggcg caggcggcgg tggggacagg aagacctgag
     9121 cccctggcgt gccgagatcc tgacacgtct gaagaagccc gcgtgtcttt tgtgcggtga
     9181 ggaagcggag gacctgagac ggtattactt ctggttcatg aacgagcaat acgcaaacat
     9241 cccgacgatg gaacggctgc aacgggcgcg cgggttctgc ctgcggcaca cccggcactt
     9301 gctgaccatc gggggaccat cccggatctc tttcgtggga gaatacgtgc tgcgagccgg
     9361 tgcggattgg ctgtgcgcgg tccagaaggg cacgacgccc ggaccgcccg aatcgcgcac
     9421 cgggagacac cggaagaccg aaggct
//
LOCUS       DBNGZC010000636         2084 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1618869, whole genome shotgun sequence.
ACCESSION   DBNGZC010000636 DBNGZC010000000
VERSION     DBNGZC010000636.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1618869"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            586..2061
                     /locus_tag="AC16G0_11055"
     CDS             586..2061
                     /locus_tag="AC16G0_11055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5100186.1"
                     /translation="MERAQLIRELGKLVGPEYVVSDPAELKVYETDGLTIFKATPDVV
                     VLPKTAEEIAEVVRLCVRERIPFVPRGAGTGLSGGALALEGGVMIGLNRMNRVLEVDY
                     ANLRAVIEPGLVNIWLTNHVSPQGYYYAPDPASQTACSVGGNVAENSGGPHCLKYGVT
                     TNHVLALELVLASGEITRIGTWAPDPPGYDLTGVFVGSEGTFGIVTKIVVRLLKRPEA
                     VKTMMLVFETIADASATVSGIIGRGIIPAAIEMMDKLSIEAVEKGTHAGYPTDAGSVL
                     LVELDGPRAEVEALTKPVIAVGRENKMREVRVAKDDVERALLWKGRKAAFAAMGQISP
                     AYYVQDGVIPRTQLLKILGEIEALSNKYGLRVANVFHAGDGNLHPLILYDDRVEGEAH
                     KAEEMGSELMQRCIAVGGSITGEHGVGFEKKDFMPLMYSPTDLEAMAKVKRVWNPDGL
                     LNPGKIFPTSKSCVEVGNARKAREIKYKQHRIEKEGLAQRF"
BASE COUNT          405 a          630 c          672 g          377 t
ORIGIN      
        1 tgcgccagtc actggtcatc tcggatggca gcatgatgat cttcctgacc cggccaatct
       61 cgctcttcct gctggttctg gccgtgatct cgatcaccaa tccctactgg aaccagatcg
      121 gcgccggcgt tcggcggttg tttgccaagc caaagtctgc gtgaaagtca ttcacgcaaa
      181 ggcccggact cagggctgag actctactag acagtgtccg gcctggattc tgcctgtcgc
      241 cgccgtacgg catcttcgtc ctgggccggc gaatccaggc catagaaaaa atcggtcctg
      301 ccagacggcg gctctggggg aaagtccttg ccagtggaga tttgctgctt taccatccgc
      361 gcatcaattc gtgattgata tcattcccgc ctgatcaccg gattcttcat ggtctttttg
      421 acctggggct gacccgagac aatccgtctg cagcccagct ccgcctgagg caacaaggcg
      481 accgccccgt ggatcgccaa gcgggcggcc acacgttcct tcttcaaaag ccgagcagct
      541 gtcccaaaga ttggaccagc ggcgtgccgt gaggggagtc actccatgga gcgtgcacaa
      601 ctcatccggg agcttggcaa gctggtgggg ccggagtacg tggtttcgga ccccgccgaa
      661 ctcaaggtat atgagacgga tggcttgacg atcttcaagg ccacgccgga cgtggtggtc
      721 ctccccaaga ctgccgagga gattgcagag gtcgtccgcc tgtgtgtccg cgagcgcatc
      781 cccttcgtcc cgcggggggc gggaacgggt ctgtcgggcg gggccttggc cctcgaaggc
      841 ggcgtcatga tcggtctgaa ccgcatgaac cgcgtgctgg aggtggacta tgccaacctg
      901 cgggccgtca tcgagcccgg cctagtgaac atctggctga cgaatcacgt gagcccccag
      961 gggtattact atgccccgga cccggccagc cagacggctt gtagcgtcgg gggcaacgtg
     1021 gccgagaact cgggcggccc gcattgcctg aagtacgggg tgaccacgaa ccacgtcctt
     1081 gccctggagc tggtcctggc ctcgggggag atcacccgga tcggcacctg ggctccggac
     1141 ccgccgggct acgacctcac gggtgtcttc gtcggatcgg agggtacctt cggcattgtc
     1201 accaagatcg tggtccgcct cctgaagcgg cccgaggccg tgaagaccat gatgctggtc
     1261 ttcgagacga ttgccgacgc cagcgccacc gtctccggga tcatcggtcg cgggatcatc
     1321 ccggccgcca tcgagatgat ggataagttg agcatcgaag ccgtggagaa ggggactcac
     1381 gccgggtatc ccacggatgc aggctcggtg ctcctagttg aactggatgg cccccgcgcc
     1441 gaggtggagg ccctcacgaa gccggtcatc gccgtggggc gcgagaacaa gatgcgcgag
     1501 gtgcgtgtgg cgaaagacga tgtcgagcgg gcgctcctct ggaaggggcg taaggccgcc
     1561 ttcgccgcca tgggccagat cagccccgcg tactacgtcc aggacggcgt catcccgcgc
     1621 acccagctcc tgaagatcct gggggagatc gaagccttga gcaacaagta cgggctccgc
     1681 gtggccaacg tgtttcacgc cggggacgga aatctccatc ccctcatcct ctacgacgat
     1741 cgcgtggagg gcgaggccca caaggcggag gaaatgggct ccgagttgat gcagcgctgc
     1801 atcgccgtag gcggcagcat caccggcgag cacggtgtgg gattcgagaa aaaagacttc
     1861 atgccgctca tgtactcgcc taccgacctg gaggccatgg ccaaggtgaa aagggtgtgg
     1921 aatccggatg gcctcctgaa cccgggcaag atcttcccca cgtccaaatc gtgcgtggag
     1981 gtggggaacg cgaggaaggc cagagaaatc aagtacaagc agcacaggat cgaaaaagag
     2041 ggactggcac agcggttctg acgggttgtc aacaatcagc tttc
//
LOCUS       DBNGZC010000637         2582 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1717391, whole genome shotgun sequence.
ACCESSION   DBNGZC010000637 DBNGZC010000000
VERSION     DBNGZC010000637.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1717391"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="AC16G0_11060"
     CDS             <1..589
                     /locus_tag="AC16G0_11060"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5100187.1"
                     /translation="LWLLADEVYERLYYGGPELGVPAPSILRKSSRDDAVMVAQSFSK
                     TFCMTGWRIGWLVARRDVAERAAQLNEFIISHATSFIQRAAQIALAQGEDEFRRMLVR
                     LKQNRDFCLAALRGMPGVTAPSPDGAFYLFPKIEGLRDSFAFCWKLLQETKVGIAPGV
                     AFGAGGEGSVRICYAAERAILEPAMERLARFLSQS"
     gene            607..1914
                     /locus_tag="AC16G0_11065"
     CDS             607..1914
                     /locus_tag="AC16G0_11065"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009058185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HIR5100188.1"
                     /translation="MSSITSRSADPGRVLSIIMGGGQGIRLFPLTRERAKPAVPLAGK
                     YRVVDIPISNCINSGMRRIYILTQFLSASLHRHVYGSFKFDHFGSGFVEILAAEQSHT
                     DTSWYQGTADAVRKNLVHFRTVDFDYALILSGDQLYRMDFRRILERHVDSKAELTIAT
                     LPVTRSAAQSLGILQLDPEGRIVRFVEKPKEPEVLDSLRLDRGLYSQFGIQDEQELLL
                     ASMGIYLFNREVLFRLLDNDLTDFGKHVIPQTLQTHRVFSYVFQGYWEDIGTIRSFFD
                     ANLDLTAELPRFNFFDMRAPIYTRPRFLPASKINGAAIEHGIISDGCIINRCSIAESI
                     VGLRCLVDEGSHITRSIIMGADYFESLESIQQNATENRPRIGIGRGTRIDHAIVDKNA
                     RIGDNCVITPGGKPATLDHPLYFIRDGIVIIPKDAVIPHGTVI"
     gene            complement(2031..>2582)
                     /locus_tag="AC16G0_11070"
     CDS             complement(2031..>2582)
                     /locus_tag="AC16G0_11070"
                     /inference="COORDINATES: protein motif:HMM:NF015382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carcinine hydrolase/isopenicillin-N
                     N-acyltransferase family protein"
                     /protein_id="HIR5100189.1"
                     /translation="VAVFANSLWAREGRNWQAPPITRRAILECATADEASDRVQKMDG
                     PAVGSFLIIDAAGRIRNLEVLPQALAVIARDGGVFVHANHCLDKDLQPHEVERLPSPG
                     SRGRRIRLHAWLEAASGRIGVETIKGLLADHSGRPEPLCRHAAKPSEWETASALIAEP
                     ASRTLHLSYGPPCEGRFVTYRIT"
BASE COUNT          457 a          870 c          785 g          470 t
ORIGIN      
        1 gctgtggctg ctggcggatg aagtctacga acggctctac tacggcggac cggagttggg
       61 ggtccccgcg ccctcgatcc tgcggaagtc cagccgcgac gacgccgtca tggtagccca
      121 gtccttctcc aagacattct gcatgaccgg ctggcggatc ggctggctgg tggctcgccg
      181 ggacgtggca gagcgcgccg cgcagctcaa tgagttcatc atctcccacg ccaccagctt
      241 catccaacgt gccgctcaga tcgcgctcgc ccagggagag gacgagtttc ggcgcatgct
      301 ggttcgcctc aagcagaacc gggacttctg cctcgccgcc ctccgtggga tgccaggcgt
      361 gaccgctccg tcccccgacg gggccttcta cctcttcccg aagatcgaag gcctcaggga
      421 ctccttcgcc ttctgctgga agctcctgca ggagaccaag gtcggcattg cccccggggt
      481 ggcgttcggc gccggggggg agggttcggt tcgcatctgc tacgccgccg agcgcgcgat
      541 cctcgagccg gccatggaac gcttggcccg ttttctctcg cagtcgtagc accgggggca
      601 acgacgatgt cctccatcac gtcccgctcc gccgatcctg gtcgggtcct gtctatcatc
      661 atgggtggcg gccaggggat ccgtctgttc cccctcaccc gcgaacgcgc caagcccgcc
      721 gtgcccctgg ccgggaagta tcgggtggtg gacatcccga tctccaactg catcaattcg
      781 gggatgagga ggatctacat cctgacccag tttctctccg cgtccctcca ccggcatgtg
      841 tacgggtcgt tcaagttcga ccatttcgga agcggcttcg tcgagatcct ggccgcggag
      901 cagagccata ccgacacctc gtggtaccag ggcaccgcag acgccgtgcg aaagaatctg
      961 gtccatttcc ggaccgtgga cttcgactac gccttgatcc tgagcggaga ccagctctac
     1021 cggatggatt tccgccgcat cctggaacgg catgtggact ccaaggcgga gctcaccatc
     1081 gccaccctcc cggtcactcg gagcgccgcg cagtctctcg gcatcctgca gctcgatccg
     1141 gagggacgca tcgtccgctt cgtcgaaaaa ccgaaagagc ccgaggtgct ggacagcctg
     1201 aggttggacc ggggcttgta tagccagttc ggcatccagg acgaacagga gctgttgctt
     1261 gcctccatgg gcatctatct gttcaaccga gaggttctct tccgcctcct ggacaacgac
     1321 ctcacggatt ttgggaagca cgtcatcccg cagactctcc agacccatcg ggtgttttcc
     1381 tacgtcttcc agggctactg ggaagacatc ggcaccatcc gctccttctt cgatgccaac
     1441 ctggatctga ccgccgaact gccgcggttc aacttcttcg acatgcgcgc ccccatctac
     1501 acgcgtccgc gctttctgcc cgcctccaag atcaacggtg ccgcgatcga gcacgggatc
     1561 atctccgacg gctgcatcat caaccgttgc agcatcgcgg agagcatcgt ggggctgcgt
     1621 tgcctggtgg atgaaggcag ccatatcacc cgcagcatca tcatgggggc cgactatttc
     1681 gagtcactgg agtcgatcca acagaacgcc acagagaatc gcccacgcat cggcatcggc
     1741 aggggcacgc gcatcgatca cgccatcgtc gacaagaatg ctcgtatcgg ggacaactgc
     1801 gtcatcacgc ctggcggcaa gcctgccacg ctggatcacc cgctctactt catccgcgac
     1861 ggcatcgtca tcatccccaa ggatgccgtc attccccacg gaaccgtgat ctgacccaga
     1921 cccgagtcgg atcgaatatt tcgaatttcg aaattcgaat ttgcggagtg cagggctagg
     1981 cggagcggct agaggttcca gggacgcgtt tgcggtgaag accgacgcag ctaggtgatg
     2041 cgatatgtga cgaaacggcc ctcgcacggg ggaccgtagc tgaggtggag cgtccgggac
     2101 gcgggttcag cgatcagggc cgaggccgtc tcccactccg acggcttggc cgcatggcgg
     2161 cagagcggct cgggccgtcc cgagtgatcg gccaacaaac ccttgatcgt ctccacgccg
     2221 atccgcccgg aggccgcctc cagccaggca tgcagacgga tgcgccgtcc ccgcgatccg
     2281 ggggaaggca gccgctcgac ctcgtgcggc tgcagatctt tgtccaggca atggttggcg
     2341 tggacgaaaa cgcccccgtc gcgcgcgatc accgccagcg cctgcggcag gacctccaag
     2401 ttgcggatcc ggccagccgc atcgatgatc aggaagctgc caaccgcggg gccgtccatc
     2461 ttctgcactc ggtcggaggc ctcgtcggcc gtggcgcact ccaggatggc ccgacgcgtg
     2521 atcggggggg cctgccagtt ccggccctcc cgcgcccaaa gcgaattggc gaacacggcg
     2581 ac
//
LOCUS       DBNGZC010000638         2773 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1325970, whole genome shotgun sequence.
ACCESSION   DBNGZC010000638 DBNGZC010000000
VERSION     DBNGZC010000638.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1325970"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /gene="mltG"
                     /locus_tag="AC16G0_11075"
     CDS             <1..835
                     /gene="mltG"
                     /locus_tag="AC16G0_11075"
                     /inference="COORDINATES: protein motif:HMM:NF014656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR5100190.1"
                     /translation="RTPWVFLSLAYLQGSLTRLQAGEYEFTSNMSLLEILHRLETGRI
                     LTHQVTVPEGFTALDIAQLLGSERLADADRFMALVKDSTFAEHLGLSVPTLEGYLFPD
                     TYRLSRGMPEEEIIRTMVARFREVLPSDLEAQAERLNLDAHGVITLASLIEKEAKLNS
                     ERPLVAGVFYNRLKRNMPLQSDPTAIYGAADSRRRITAIDLQRKTPYNTYLKAGLPPG
                     PIANPGLASIRAALNPARVDYLYFVAKNDGSHFFSRTFEQHVEAVRNYQMKGKDPAGR
                     S"
     gene            908..1666
                     /locus_tag="AC16G0_11080"
     CDS             908..1666
                     /locus_tag="AC16G0_11080"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5100191.1"
                     /translation="MRGRVGMVGIGVAMCALAACAGPESEQARQESARLHYDIGIGAL
                     AENNTTKAISELQTAVQEDPQNARGFHALGVAYFRSDQNDRAIAAFRKAVELNPRLSD
                     VYTDLGVAYMRQQTWDPAIDAFRKATANPQNTNPERAYMYLGDIYYIRKQYDLAAEEF
                     RKLQDVLPQSPDGYFLLGRTLLAQGKVAEAREQIEKAIKINNAIPTFHLELGMALMRE
                     GNRTGARQSFRRVLELTPSGREAEEARRYLRELN"
     gene            1699..2220
                     /locus_tag="AC16G0_11085"
     CDS             1699..2220
                     /locus_tag="AC16G0_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100192.1"
                     /translation="MTTPSPSAGAGWAESLQQLARHVPGLGGYQDREGLRETDKQVRM
                     YLAGLIADLPLSLECAERRLEEARQLDRLPSLDRVSRVLRRLADRVRFSSYGFAGVFD
                     LHKIREVELAALHRFDLRLLEALPRLQERVSALADSAMDEARFPQALQAMETALQEFE
                     GSLAERDKLAHSL"
     gene            2349..>2773
                     /locus_tag="AC16G0_11090"
     CDS             2349..>2773
                     /locus_tag="AC16G0_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR5100193.1"
                     /translation="MALLMEIIEWFDPDGRQWVHRIPEEGSADIKMGAQLIVRESQAA
                     VFFRDGKGLDVLGPGRHTLSSLNLPILTRVLSLPWGFTSPFRTEVYFVNQKVFTDLKW
                     GTKEPVAFKDKELGLVRLRAFGIFTARITQPLLFINTLVG"
BASE COUNT          514 a          884 c          892 g          483 t
ORIGIN      
        1 ccgcactccc tgggtttttc tctccctggc atacctacaa gggtcgctca cacgcctgca
       61 agccggcgaa tacgagttca cctccaacat gtccctgctc gagatcttgc accggctcga
      121 gacgggacgt atattgaccc accaagtcac cgtccccgag ggattcaccg ccctggacat
      181 cgcccagctt ctcggcagtg agcggctcgc ggatgccgat cgctttatgg cgctggtgaa
      241 ggattcgaca tttgccgagc atctgggttt gtccgtccct accttggagg ggtacctgtt
      301 tcccgatacc taccgtctca gccgggggat gcccgaggaa gaaatcatcc gcaccatggt
      361 cgcccgcttt cgcgaggtcc ttccgtccga cctcgaggcc caggcggagc ggttgaatct
      421 ggatgcgcac ggggtaatca ccctggcctc cctcatcgag aaagaggcaa aactcaacag
      481 cgagcgcccg ctggtggccg gggtgttcta caaccgactc aaacgcaaca tgcccctcca
      541 gtccgatccc acggcgatct acggggccgc cgactcccgt cgccggatca cggcaatcga
      601 tctgcagcgc aagactccct acaacaccta cctcaaagct gggctgcctc ctgggcccat
      661 tgccaatcct gggctggcct ccatccgggc ggccttgaac ccggcccgcg tggactacct
      721 gtacttcgtc gccaagaacg atgggtcgca tttcttttcc cggacgttcg agcagcacgt
      781 ggaagccgtg cgaaattatc agatgaaggg gaaggatccg gccggccgct cctgaattcc
      841 ctggacgcgt tttacgcgcg gtcgggtgca ggctggaacc tgggtctcgt gggaacggga
      901 ggaccgaatg cgaggaagag tcgggatggt cgggatcggg gtggcgatgt gtgccctggc
      961 ggcatgcgcg gggcccgagt cggagcaggc ccggcaggag tcggcccggc tccactacga
     1021 catcggcatc ggggcgctgg ccgagaacaa cacaacgaag gccatcagcg agctccagac
     1081 cgcggtgcaa gaggacccgc agaacgcccg cggctttcat gccctggggg ttgcctactt
     1141 ccgttccgat caaaacgacc gtgccatcgc ggcgttccgg aaggcggtgg agctgaatcc
     1201 acgcctgtcg gatgtctaca ctgacctggg cgttgcctac atgcggcagc agacgtggga
     1261 cccggccatc gacgccttcc gcaaggcaac ggccaacccc cagaatacga atccggagcg
     1321 cgcctatatg tacttggggg acatctacta catccggaag caatatgacc tggcggcgga
     1381 agaatttcgc aagctgcagg acgtactgcc ccagtccccc gacggctatt tcctgttggg
     1441 caggacgctc ctagcccagg gaaaggtggc ggaggcccgg gaacagatcg agaaagccat
     1501 caagataaac aatgcaattc ccacattcca tctcgagctc ggcatggcct tgatgcgcga
     1561 ggggaaccgc acgggggcga ggcaaagctt ccgccgtgtc ctggagctga ccccgtccgg
     1621 ccgtgaagcg gaagaagccc gacggtacct gcgcgagttg aactagccgc catcccgcgg
     1681 tgccgcaagg aggcgcccat gaccacaccc agtccgtctg ccggcgccgg ctgggcggag
     1741 tcgctccagc aactcgctcg ccacgttccc ggactcggcg gataccagga ccgggagggc
     1801 ctgcgggaga cggacaaaca ggtgcgcatg tatctggcgg ggctcatcgc agaccttccg
     1861 ctcagcctgg agtgcgcaga gcgtcgtctg gaagaggcgc ggcaactgga tcggctgccg
     1921 tccttggacc gggtgtcgcg cgtcctccgc aggctggcgg accgagtgcg gttctccagc
     1981 tatggatttg cgggagtctt cgatctccac aagattcggg aggtcgagct ggcggccctc
     2041 catcggttcg accttcgcct tctggaggcg ctgccgcgcc ttcaggagcg cgtctcagcc
     2101 ctggcggact ccgccatgga cgaggcccgc ttcccccagg cgctgcaggc catggagacc
     2161 gctctccagg agttcgaggg gagcctggca gagcgagaca agctggccca cagtctgtag
     2221 cccggcgccg agcagcgcct gaccggcccg gcctgttcga gcaggctccg gtgcaaggcc
     2281 agcgcggcat cggggccggc gggattgggg tgtaggtcgt tgacacaggc tggtgagaag
     2341 gaggcggcat ggcccttctg atggaaatca tcgaatggtt cgatccggat ggccggcagt
     2401 gggtgcaccg aatccccgag gagggctctg ccgacatcaa gatgggcgcg cagctcatcg
     2461 ttcgggagag ccaggcggct gtgttcttcc gagatggcaa aggcttggat gtgctcgggc
     2521 ctggccggca cacgttatcc agcctgaacc tgcccattct gacgcgggtc ctcagcttgc
     2581 cctgggggtt caccagccca ttccgcaccg aggtatactt cgtcaaccag aaagtcttca
     2641 ccgacctcaa atgggggacg aaggagccgg tggccttcaa ggacaaggaa ttggggttgg
     2701 tgcgcctgcg ggccttcggg atcttcacgg cgcggatcac gcagccactc ctgttcatca
     2761 acaccctggt cgg
//
LOCUS       DBNGZC010000639         3198 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_446955, whole genome shotgun sequence.
ACCESSION   DBNGZC010000639 DBNGZC010000000
VERSION     DBNGZC010000639.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_446955"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="AC16G0_11095"
     CDS             complement(<1..1383)
                     /locus_tag="AC16G0_11095"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR5100194.1"
                     /translation="MRGKGYVGKILLIDLTARKVETQTLPDALYKKYLMGSGMGARLL
                     TDLGDLAADPFDPRNPLVIMPGMFAGTTIPGGTRTSFVSKSPLTGIWGEATVGGTWGS
                     EFRCCGYDGLVILGAASSPVYLWINNDHVEIRSAEKVWGKDTYDAGDHLLVETDPKAK
                     VAAIGQAGENLVRFAAVMVEGRHARAAGRTGMGAVMGSKQLKAIVVRGTRGVPVYNPT
                     GLMGWAFKKTNEMPAKFGMFTVYGTSGSIEVHEERGGLPIKNFQDGHWPAGAAKISGK
                     TIHEKYDVKSSSCSGCPVHCWQHVTIEGEDGVRTNVGHNPEYETLGAFGAMCLNEDLP
                     SVIRANELCNRYGLDTISTGNSIAFAMEAYERGLISKADTGGLEIAWGDGNAILTLVE
                     QIAFKQELGGLLADGSFAAARKIGGDAEKLTITVKGLEFPMHDPRAFWSMGINYATGA
                     RGACHLEGLPF"
     gene            2249..>3198
                     /locus_tag="AC16G0_11100"
     CDS             2249..>3198
                     /locus_tag="AC16G0_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100195.1"
                     /translation="MSKKGNRRTAATTALVAMSMLVSTPWLVATAQSPPRSINSTSQG
                     RSSTPSTLVAEQPPPPSQAQQRLINFQVLGANDLGMHCGDFDHRNVSILPPFNTLHAQ
                     VIKKGALPQILDPTQAQVVYSAASNPADPAVNNPIPSSVYKTDFWDLNPRTGNSIAFD
                     AYDAHYPPWILSLFPLSADLGLPVPDLQRLYLGDHQLTADQQAMPSLTAPPAFTQPFA
                     ANTPQAFKLFYASFPFFLNFPFGYTQPGVNWFSAEGIPVAPFDDQGRSNPFPLMRVQA
                     LASPGNAQGLTAGTVMSTLDTPTPVSGELNCKACHTSAQDG"
BASE COUNT          592 a         1097 c          920 g          589 t
ORIGIN      
        1 gaaggggagc ccctccagat ggcaggcgcc tcgcgcgccc gtggcgtagt tgatccccat
       61 agaccagaag gctcggggat cgtgcatggg aaactccaga cccttcaccg tgatcgtgag
      121 cttctcggca tcgcctccga tcttgcgggc cgcggcgaag gagccatccg ccagaaggcc
      181 ccccagttcc tgcttgaagg cgatctgctc gaccagcgtc aggatggcgt tcccgtcgcc
      241 ccaggcgatt tccagccccc ccgtgtcggc cttggagatc aggccgcgct cgtaggcctc
      301 catggcgaag gcgatgctgt tgcccgtgga gatggtgtcc agcccgtacc ggttgcacag
      361 ctcgttggcg cgaatcaccg agggcaaatc ctcgttcagg cacatggcgc caaaggcccc
      421 cagggtctcg tactccgggt tgtggcccac gttggtccgg acgccgtcct ctccctcgat
      481 ggtcacgtgc tgccagcagt ggacggggca gccggagcac gagctcgact tcacgtcgta
      541 cttctcgtgg atggtcttgc cgctgatctt ggcggcccct gcaggccaat gcccgtcctg
      601 gaagttcttg atgggcagac cgcctcgctc ctcgtgtacc tcgatgctcc ccgaggtgcc
      661 atagacggtg aacatgccga acttggccgg catctcgttg gtcttcttga acgcccagcc
      721 catcaggccc gtcggattgt aaaccgggac cccgcgcgtc ccccggacga cgatggcctt
      781 gagctgcttc gaccccatca ccgcgcccat tccggtccgc ccggcggcgc gggcatggcg
      841 gccctccacc atgacggcgg cgaaccgcac cagattctcg ccggcctgcc cgatggccgc
      901 taccttggcc ttgggatcgg tttccaccag caggtgatcg ccggcatcgt aggtatcctt
      961 accccagact ttctcggcgg accggatctc cacgtggtcg ttgttgatcc aaaggtacac
     1021 agggctggat gccgcaccca ggatcaccag gccatcgtag ccgcagcagc ggaattcaga
     1081 gccccacgtg ccacccacgg tggcctcgcc ccagatgccg gtgaggggag acttggatac
     1141 gaaggaggtg cgggtcccgc ccggaatggt tgtcccagcg aacatcccgg gcatgatgac
     1201 gagcgggttc cgtggatcga aaggatccgc ggccaggtcg cccagatcgg tcagcaggcg
     1261 ggcgcccatg ccgctgccca tgaggtactt tttgtacagc gcgtctggaa gagtttgcgt
     1321 ctcgaccttt cgcgccgtga gatctatcag gaggatcttg ccgacatagc ccttgcctcg
     1381 cattgctact cctctatgtg ggttccattt ctgaaccgaa aatcaaaggg atacaaatgg
     1441 cgaccgatat aatccggtat caagcatgac ggcgcaagga aaaaggcagg gagttttggt
     1501 acgaccacga gccttccgta tcatctcagc gtgaagcagg gggtcatcct cctccggtgt
     1561 ggttccggtt tgaaggagtt atgcgctggc ggcggaccgc tagtcatcgt ccggaggggc
     1621 ttcgaatctc cgcggacgcc aaagaggtac gaatcgatgc agggcaaggc aacggacggt
     1681 cgtgtgaccg gaagcaggca ctgcaaggga taggattcag agacggagcg gaagagggaa
     1741 gtttattcac tctctcagtg taactcttac accattgtcg cggtgactcg atcatctcgt
     1801 cagcggccga cgcgtgataa gtactatgaa acccgcgaat ccctcagtgc cctctttctt
     1861 ggcatcgtga ttgctccgag aagagcccac cattccacgg ccccgagaac atcgcagaac
     1921 cgagggatga gcctcaccag accgtgtgtg gctgatcggc gttgccgtaa tgctccacct
     1981 accccatcca gcaccgactc cccgacctca tgacgcgcga cacgatttgc cggcgcctac
     2041 gcatgtgcac gctcgggcaa gaaccccctc gcatgttcac gccccgtgcg ggtgttgcgc
     2101 cggtgccgcc atgccgacac aggagtcctg cgcatcgaga gatccccctc ggtcgctcgc
     2161 gacttactga atcaggtgat caccacggca aagatgcccg cgccacggcg attgcggaaa
     2221 tgcccggccg gacagagagg ggaataccgt gagcaagaag ggaaaccgga gaaccgccgc
     2281 gactaccgcg ctggtcgcca tgagcatgct cgtctcaacc ccttggctcg ttgccaccgc
     2341 ccaatctccc ccgaggtcga tcaactcgac cagccagggc cgatcctcca ctccctccac
     2401 tcttgttgcc gaacaacccc cgccgccctc gcaggcccag cagcgactca tcaatttcca
     2461 agttctcggg gccaacgatc tcggcatgca ctgtggggat ttcgaccatc gcaacgtgag
     2521 catcctgccg ccgttcaaca cgctccacgc gcaggtgatc aagaagggtg cgttgccgca
     2581 aatcctcgac ccaacgcaag cgcaggtggt gtactcagcc gcctcgaacc ccgcagaccc
     2641 cgcggtcaac aaccccattc cgtccagcgt ctacaagacc gacttctggg atctgaatcc
     2701 ccgcactggc aactccatcg ccttcgacgc gtacgacgcc cattatccgc cgtggatcct
     2761 cagcctgttc ccgctgtccg cggatctcgg gctgccggtc cccgacctcc agcggctcta
     2821 cctgggggat catcagctga ctgccgacca gcaggccatg cccagtctga ccgccccgcc
     2881 cgccttcacc cagccattcg ccgcgaacac cccacaggct ttcaagctgt tctacgcgag
     2941 cttcccgttt ttcctgaact tcccgttcgg ctatacccaa cccggcgtta actggttctc
     3001 ggccgaaggg atcccggtcg cccccttcga cgatcagggc cggtccaacc cgttccccct
     3061 gatgcgcgtc caggcactgg cgtcgcccgg caatgcccag ggcctcacgg ccgggaccgt
     3121 catgtccacc ctggacaccc cgacgcccgt ctcaggagag ctgaactgca aggcctgtca
     3181 cacctcggct caggacgg
//
LOCUS       DBNGZC010000640         2617 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2403728, whole genome shotgun sequence.
ACCESSION   DBNGZC010000640 DBNGZC010000000
VERSION     DBNGZC010000640.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2403728"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..629)
                     /locus_tag="AC16G0_11105"
                     /pseudo
     CDS             complement(1..629)
                     /locus_tag="AC16G0_11105"
                     /inference="COORDINATES: protein motif:HMM:NF022077.7"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
     gene            complement(860..1414)
                     /gene="folE"
                     /locus_tag="AC16G0_11110"
     CDS             complement(860..1414)
                     /gene="folE"
                     /locus_tag="AC16G0_11110"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HIR5100196.1"
                     /translation="MIEDLVKDLLKELGEDPYREGLAKTPERMAKALQYLTSGYDKTV
                     DEVLHGAVFTEAYDEMVVVKDIELYSLCEHHILPFFGKVHIAYMPAGKIVGLSKLARL
                     VEMFARRLQVQERLTTQIAEALQGAIQPSGVAVVIEAFHFCMMMRGVEKQNSKAITSC
                     MLGSFRTCRETREEFLQLIHAHRQ"
     gene            complement(1411..1884)
                     /locus_tag="AC16G0_11115"
     CDS             complement(1411..1884)
                     /locus_tag="AC16G0_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HIR5100197.1"
                     /translation="MPEPGSPMVEVTRTFRFSAAHRLNNPRFSPAENARLYGRCNNPN
                     GHGHTYRLEVTIRGPVSPDTGWVEGWPRLEAVVGAAVLDRLDRSNLDHEITPQDGPTS
                     TTEALLGLIWRLLDGRLPAGRLHRLRLEETPNNFFELDRDGLAPARGPREKVSPT"
     gene            complement(1877..2299)
                     /locus_tag="AC16G0_11120"
     CDS             complement(1877..2299)
                     /locus_tag="AC16G0_11120"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl trahydropterin synthase family
                     protein"
                     /protein_id="HIR5100198.1"
                     /translation="MIRLTKRVEFCAAHRYINPRWNEEENRLVFGKEGNPDGHGHNYV
                     LEVTILGEPSPDTGMVMDLKALKDLIVETVVDRFDHKHLNLDVPYFKEKVPTPENLAV
                     VIWELLVSRLEGCRLDRIRLYEDDSTFVDYAGERDERA"
     gene            complement(2296..>2617)
                     /locus_tag="AC16G0_11125"
     CDS             complement(2296..>2617)
                     /locus_tag="AC16G0_11125"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5100199.1"
                     /translation="AGRRAAVPETVTDGDTGLLVDPDDPAALAEALAKLLQDGELRRV
                     MGEAGRRRVERYAAPRVAAAFVSTVQAALKDRSEGIGSQAEPLESSAREGHPAGIAGA
                     GGRR"
BASE COUNT          461 a          855 c          822 g          479 t
ORIGIN      
        1 gggcacacga aggtgctcat gttttcgatg atgccgagaa ttggcacctt gagctgttga
       61 aacatggcga tggccttgct ggcgatggtc atggccacat cctgcggcgt catgatcacc
      121 acggcgcccg agaggggaat ggattgcgcc agggtgagtt gagcgtcgcc cgtccccggc
      181 ggcagatcga tgaggagata atccagtcgt ccccactcca catcccgaag gaactgttgg
      241 atcgcccccg agaccatggg tcagcgccaa accgcgggtg caccctcggg catgaggaag
      301 cccagggaca tcaactggac accatgcccc aggatcggga agatcttttg cccgttgctc
      361 tggggcatcc ggttgacacc ggtcatcatg ggaatgctgg gaccgtagat gtcggcgtcg
      421 agcagaccgg tagaagcacc cgtctgcgcc agagccaccg caaggttcac ggcgatggtg
      481 gatttgccaa caccgccctt gcccgacccg atggcgatgc tgtgtccgat cccattcagg
      541 ctcgccggcc cacccatcgg ccccggaccg gctggttgtg ctgtcatgac cgctccgtcc
      601 cggcgccgtc ccgagccgcc ggccgccatc cgtccccacc cggcagagtc aaaccgtgga
      661 gcgtcctcat gccagatccc gtgacagcgc gtctcttgat gcgcgcatca caggtctaga
      721 taccagggca atgccggggt gtcaagcgcc aaggggagga tgattggagt cacgagccga
      781 gggtggagag acgcaactgc gatctggacg gtggacgcgg aaggccgagg gttgatccct
      841 gacggccgat ggcgttggct cactgccggt gagcatggat gagctggaga aactcctcgc
      901 gcgtctcccg gcaagtgcga aacgacccca gcatgcagga ggtgatcgcc ttgctgtttt
      961 gtttctccac gccccgcatc atcatgcaga aatgaaatgc ctcgatgacc acggcgacac
     1021 cagaaggctg gatggccccc tgcagggcct cggcgatctg ggtggtgagc cgctcttgca
     1081 cctgcaggcg acgggcgaac atctccacga gcctggccag cttgctcagg cccacgatct
     1141 tcccggcagg catgtaggcg atgtggacct ttccgaagaa gggcaaaatg tggtgctcgc
     1201 acagggagta aagctcgata tccttcacca ccaccatctc gtcgtatgcc tccgtgaaca
     1261 cagcaccgtg gaggacctcg tcgaccgtct tgtcgtatcc tgaggtgagg tactggagcg
     1321 ccttcgccat ccgctccggc gtcttggcca aaccttcacg gtacgggtcc tcgccgagct
     1381 ccttcagcag atctttgacc agatcctcga tcatgtcggg ctcactttct ctcgcgggcc
     1441 gcgggctggc gccaggccgt cgcggtccag ctcaaagaag ttgttgggtg tctcttccag
     1501 gcgtagacgg tgcaagcgac ctgccggcag gcggccatcc aggaggcgcc agatcaggcc
     1561 aagcaacgcc tccgtggtgc tggtgggtcc atcctggggg gtgatctcgt gatccaggtt
     1621 ggatcgatcc aggcggtcca agacggccgc ccccaccacc gcctccagcc tcggccagcc
     1681 ttcgacccat ccggtatcag gcgagaccgg gccgcggatc gtcacctcca gccgataggt
     1741 atggccgtgg ccgtttggat tgttgcagcg gccgtagagc cgcgcgttct cggccgggct
     1801 gaaccggggg ttgttcagcc ggtgggccgc agagaatcga aaagtgcgcg tgacttccac
     1861 catgggggat ccgggctcag gcacgctcgt ctcgctcccc ggcgtaatcc acgaaggtag
     1921 aatcgtcctc gtacaggcgg atccggtcca gccggcagcc ctccagtcgg gacaccagca
     1981 actcccagat gacgacggcc agattctccg gagtcgggac cttttccttg aagtagggga
     2041 catccaggtt gagatgcttg tgatcgaagc gatccaccac ggtctcgacg atcaggtcct
     2101 tcagggcctt gagatccatc accatcccgg tgtcgggcga tggctctccc aggatcgtga
     2161 cctccaagac gtagttgtgg ccgtgaccgt cggggttccc ttccttgccg aaaaccaatc
     2221 ggttctcttc ctcgttccag cgggggttga tgtaccgatg agcggcgcag aactccactc
     2281 gcttcgtgag tcggatcatc gtcggcctcc tgcgccggca atgcctgccg ggtggccttc
     2341 cctcgccgaa gattcgagcg gctccgcctg cgacccgatt ccttccgagc ggtccttcaa
     2401 ggcggcctga accgtgctca cgaaggctgc cgccacgcgc ggggcggcat agcgctccac
     2461 cctgcggcga cccgcctccc ccatgacgcg gcgcaactcg ccgtcctgca ggagcttcgc
     2521 cagggcctcg gccagggcgg cgggatcatc cgggtccacc aagagccccg tgtccccatc
     2581 ggtcacagtc tctgggacgg cagcccgtcg gccggcc
//
LOCUS       DBNGZC010000641        13048 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_153468, whole genome shotgun sequence.
ACCESSION   DBNGZC010000641 DBNGZC010000000
VERSION     DBNGZC010000641.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 13048)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13048)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13048
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_153468"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..1743)
                     /locus_tag="AC16G0_11130"
     CDS             complement(274..1743)
                     /locus_tag="AC16G0_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003389615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR5100200.1"
                     /translation="MALSPVNLLWALVGCLMGTLIGVLPGIGPSSGIAILLPLTTVVG
                     PIPAIIMMAAIYYGAMYGGSTTAIVVNIPGEASSVPTCLDGYELAKQGRAGPALGIAA
                     IASFVAGTLSLVGLTFFAPILANVALAFGPPEYFALMFMSLSLVISLSGRALLKGLIA
                     TVLGLLAAMVGQDPLTGAARLTFGSEEMMAGVNFISVIIGLFAIGEVLVNVEEAVTYI
                     YEKKKLDWMPTWADIRQCWGAMLRSTGIGFFLGLLPGCAPAITTFVAYDLEKRVSKHP
                     ERFGKGAMEGVAAPEGANNATCSGGFVPLFAFGLPTGPALAVLLGGLMMYGLQPGPML
                     FQQNPQFVWAVIASMYIGNVILLVLNLPLVGMWARIALLPFPVLGPLILVFSVIGAYS
                     IRFLAFDVWVALLFGVIGYLMRKFGFPMAPMVLASVLAQMLETSLQQTLLLSQGSPLI
                     FITRPIAAGFTALAFVSIIRGVWLELRAKAPEVAEEDEA"
     gene            complement(1793..2257)
                     /locus_tag="AC16G0_11135"
     CDS             complement(1793..2257)
                     /locus_tag="AC16G0_11135"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR5100201.1"
                     /translation="MRTQRGADFASGLFLSLLGLLVILAARRIAGSPDVRLEPRTLPM
                     ILGWTILVAGAILAVHAWHFQGEDRSIKWPNRAGTRRIAVTLLSLTAYLILMIPLGLP
                     LGTVLFVAFLVWYLGRYRAIWAAALGLVSGATVYYVFIRLLELSFPAGPLGR"
     gene            complement(2276..3268)
                     /locus_tag="AC16G0_11140"
     CDS             complement(2276..3268)
                     /locus_tag="AC16G0_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR5100202.1"
                     /translation="MRTPRSTPRSLIRLPWIAILAMTLPLGIGPAGSQAEAQEKYPAK
                     TITYLVTFDPGGQSDREARRQQPHLERLLGQKVLIDYKVGGGGALGWRELVKSKPDGY
                     TIGGFNVPHIILQPMQQEVGYKTEQIVPVALFQRTILALAVLNTSQFKTLQDFIDYAK
                     KNPGALTVGGSATFSGPHVTTLRFEKLTGTKVTYVPFTGAAPQTMAFLGGHTAAAISN
                     SDDLVRYKDKVRVLAFASEARFPDFPDAPTFKEQGIDLQEAVDRGVGVPAGTPDAIIQ
                     RLEAAFLEICRNPEIQVEMKKQGFVPLAMGHKETKAYVEKMAGIYRELMAGIKK"
     gene            complement(3850..4698)
                     /locus_tag="AC16G0_11145"
     CDS             complement(3850..4698)
                     /locus_tag="AC16G0_11145"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR5100203.1"
                     /translation="MWLLPLALVLCSAGLHASWNLIVKSEVDKLFSGWLTVLAPSLIL
                     SPALFVTGLPPREAWPFLLGSGALESLYVVALTRAYTYGDLSVVYPVARGLAPVFVAM
                     AAPIALGERLSPLAMLAILLVGGGISWLGFSAGRSATRVAALLWAVTTAALIASYSIV
                     DKAGVSRSHPLAYIITLFGLTAVIMTPYVLQQREFQQLRAIWRLRWRILVAGGLLSFG
                     AYLLVLIAMRLTQVSYVAALRESSVIIATLLGWRVLKEGFGGRRLAASTLVALGLFLL
                     VLAMRG"
     gene            5120..5887
                     /locus_tag="AC16G0_11150"
     CDS             5120..5887
                     /locus_tag="AC16G0_11150"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR5100204.1"
                     /translation="MAVPAGRRRISPLGHSPGIAPIAYRTIAEQVAGSIREAIAQGSI
                     PPATRLLEAPLAREMGTSRAPVREALSQLEREGWVVKKPNRGARVLELTEETIREVAS
                     LRGVLEGFAASLAVDRLTAKDFKALEDGLAEMDRAAHRGQFPRLLELDFQFHAFICQA
                     SGHRILYQTWNGMAGKIRLFLSATNLMYQDLSRIVRGHKLILEALKSRDKAKACRVMG
                     EHLGEMLDPFIAKLVSSRARAYRGRARNLQVHAGQGG"
     gene            5967..6785
                     /locus_tag="AC16G0_11155"
     CDS             5967..6785
                     /locus_tag="AC16G0_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR5100205.1"
                     /translation="MKRSRLWMGVLAVGLVMTGLAWSVPVQAAELQDTLNAVIKKGEL
                     TVGTFDFFPPWGFRDAQNNIVGMDVDIAKELAGDDVLKVKLTLVPVPTTQDRINFLLS
                     GKIDVAISNFTATLPRSKAITFTDPYVIDGVGAIFRKEHKIADWKDLNGKRVAVTTGS
                     TGEILVKKQAPQAKLLQFDQASTALLAVQQNKADAQIEDYTYTVYHAGRNKDLAYLNK
                     PLDFQPYCFGVRKFDQEWLNYLNFFIYRLQQSGKMKELYKKWFNAEPFHVSPTW"
     gene            6850..7518
                     /locus_tag="AC16G0_11160"
     CDS             6850..7518
                     /locus_tag="AC16G0_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011751297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR5100206.1"
                     /translation="MHGYVFQFRPIFQELPLLLSGALVTIHISILTLLFSFIVGTIGA
                     LCRLSRFRWLNVPARWYVEFIRNTPLLVQLFFIYFGFSQINIEVSNYMAGLIGLVVNN
                     GAYLTEIVRAGIEAIHKGQVEAGLSLGLSFPQLMRYVVFPQAFRVIYPPLCNQFIGII
                     LWSSLVSTIAVQDLAMRGKELASETFRSFETYAVVTLVYVVMTLTISGCLKVLGRRLF
                     RRPA"
     gene            7571..8233
                     /locus_tag="AC16G0_11165"
     CDS             7571..8233
                     /locus_tag="AC16G0_11165"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR5100207.1"
                     /translation="MPQYVQDSLNLYDAVLFLQGVWNTVALCVVGLGLGTLIALVVGL
                     SRASHLRWLSWPAGLYIEIIRDSPLLMQLYLVFFALPLLGLQVPIFVAGVATLMVNSS
                     AFMAEIVRAGIQAVAKDQWDAARGLGMGYLQAMRWIVLPQAFRVMIPPGVGLLIGLIK
                     DSSIISVISYLELTRVGQILTEKTFLSFQVFGIVAALYFLLCYPLSRLALYTERRLKV
                     SG"
     gene            8226..8948
                     /locus_tag="AC16G0_11170"
     CDS             8226..8948
                     /locus_tag="AC16G0_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007930250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR5100208.1"
                     /translation="MVKVKDVHKYFGHLHVLKGLNLEVARGEVVVIIGPSGSGKSTLL
                     RCLNYLEPVQSGEIWIDGIRLDHHKTNIRQVRAEVGIVFQAFNLFPHLTAMRNVMLAP
                     LVVRKRREDEVRREAVTLMTKVGLQDKIDAYPAQLSGGQQQRVAIARALAMQPKLMLF
                     DEVTSALDPELVREVLDVMKQLARDGMTMMVVTHEMSFAREVADRVIFMDEGRIVEEG
                     TAADVFDRPRSQRTQEFLGKIL"
     gene            9003..10394
                     /locus_tag="AC16G0_11175"
     CDS             9003..10394
                     /locus_tag="AC16G0_11175"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase family protein"
                     /protein_id="HIR5100209.1"
                     /translation="MVDVNETTRQAVIGWDLSRVVFRDLERDYPVVERGEGIYLFDTK
                     GRKYIDGSGGSSVVTSIGHGNKRVAAVMAKQAETIAFSPAHCFANQPHIELCQKIAEI
                     APKGMGHTFLLSGGSEATENAVKFARQYEFVRNNRGKYTVIGRWQGFHGNTFGAMTVS
                     GITYRRQIYGPMMGHAVKIPPCYCYRCDWGLTYGNCNLECARALKKVIRQEGRENVLA
                     FIAEPVVGAALGAVPAVPEYFPLIREICDEFDVVFIADEVMTGFGRTGANFGVDHWQV
                     TPEIIATAKGISGGYTPLAAVLLRDTIIDTLRAHHSNFRGGHTYCGNPLSARIGMEVL
                     AIIREQNLIENSRRMGLRLLEGLRKLLDHPTVGDVRGKGLMAGVEFVRNKQTKEPFPV
                     EYQFGKRIFEEAFARGLVVFAGSGSVDGVAGDHILLAPPLIITEGQVDDFLAILDEAI
                     GVVEKALPADLRG"
     gene            10495..11829
                     /locus_tag="AC16G0_11180"
     CDS             10495..11829
                     /locus_tag="AC16G0_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007954818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase family protein"
                     /protein_id="HIR5100210.1"
                     /translation="MSSQVYRDFTKTYPVMVRGKGVCLYDTGGREYLDAVGGIAVVNV
                     GHGVPEIITAMTEQAQKVAFVHGGSFANEPAQALAEELAQWAPRGLGHVLLLSGGSEA
                     TETAMKLAHQYHLERGKPGKYRIISRWISYHGNTIGALSMSGRTAWRREFTPYLLSFP
                     KIHPPYCYRCPYGRTYPTCGIACAEDLERVITLEGADSIAAFIAEPVIGTSAAGVTPP
                     PEYYPRIREICDKHDVLFIADEVINGIGRTGKNFGIEHWGVIPDMITTAKALSSGYAP
                     LAAVLLHDKVYDAIARGSGHTAQGFTYSGHPLCSAVGLAVLRYLRAHKLVEHAARIGH
                     RLLQRLESLKRFSIVGDVRGTGLILGVEFVADQATKRPFPPEAGVTRRIVQHTLAEGV
                     MVVPGMGGMIDGVSGDHIQISPPYIFDEANVEQLVTALEVAIQKVMHEVPVR"
     gene            11846..12706
                     /locus_tag="AC16G0_11185"
     CDS             11846..12706
                     /locus_tag="AC16G0_11185"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR5100211.1"
                     /translation="MNSHGMHETGSEPYFYSNYTWRELREVPAERRVVLLPVGSTEDH
                     GHHLPLDTDNYLAWTVCVETAKRIPDEVLVMPLIPYGYNLHHIDFPGTICVAAEHFIN
                     YVSDVCQSVAYHGFKRILIVDGHGSNVPLLDVVARRVILQTDALCASFIYTNLIKDVC
                     RRIRQSVHPGGMAHACELETSLFLYLNEQRVRKAEIQKEVNLPPSKYIWLDLVDGSPL
                     QLMDWWSTFSKTGVVGDPTLATREKGEQMFTAVTERMVELVREFRDRPREPRHDMHAE
                     TPGDLFGLHP"
     gene            12774..>13048
                     /locus_tag="AC16G0_11190"
     CDS             12774..>13048
                     /locus_tag="AC16G0_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5100212.1"
                     /translation="MTTQNDARFAREEYLRHIMLDFRQMTDFVKEPFLVAKADGIRFW
                     DVQGKEYLDGLAGIFVVNVGYNNPRVLEAVRRQLETLPFNPPMHGTNP"
BASE COUNT         2423 a         4091 c         4284 g         2250 t
ORIGIN      
        1 ggcggcccct ccgccctctc ggccgcgaag agatgacgca cctcgacgcc gccctcaaag
       61 gagagggcct ctgatgcgcc tccggcccgc gtcatatcgc ctaccttagc ctcgatcacc
      121 gcgatggggt gatccagtcg gaagatgaga agatagtccg ttttcgtcac gggccgccgc
      181 aaatgtcttc tgcctgccac caccattcgg cgcccgtgaa gtacttgtgc tcgtggactt
      241 cctcttccgt cctgccggcc cggcgcaagc aattcaggct tcgtcctcct ctgcgacctc
      301 gggcgccttg gcccgcagct ccaaccacac accccggatg atcgaaacga atgccagcgc
      361 cgtgaatccc gcggcgatcg gacgggtgat gaagatgaga ggcgatccct gggaaaggag
      421 cagggtctgc tggagagaag tctccagcat ctgggccagg acgctggcca agaccatggg
      481 ggccatgggg aacccgaact tccgcatgag gtagccaatc accccgaaga gcaaggcgac
      541 ccacacgtcg aaggccaaga agcgaatgct gtaggcgccg atcaccgaga agacgaggat
      601 caggggcccg agcacgggga acggcaagag cgcgatgcgg gcccacattc cgacgagggg
      661 aaggttcagc accagcagaa tgacgttgcc gatgtacatg ctggcgatca ccgcccacac
      721 gaactggggg ttctgctgga agagcatggg gccgggctgg agcccgtaca tcatcagtcc
      781 gccgaggagg acggccaggg cgggacccgt gggcaggccg aaggcaaata agggaacgaa
      841 cccaccactg caggtggcgt tgttcgcccc ttccggggcc gcgaccccct ccatggcccc
      901 cttcccgaaa cgctcgggat gcttcgacac ccgcttctcc aggtcgtagg ccacgaaggt
      961 ggttatggca ggagcgcatc caggcaggag gcccagaaag aatccgatgc cggtggaccg
     1021 gagcatcgca ccccagcatt ggcggatatc ggcccaggtt ggcatccagt ccagcttctt
     1081 cttctcgtag atgtaggtca ccgcctcctc gacgttcacc agcacctcgc cgatggcgaa
     1141 aaggccgatg atcacgctga tgaaattgac cccggccatc atttcttccg atccgaaagt
     1201 cagcctggcg gcacccgtga gcgggtcctg gcccaccatg gccgccagga gtcccagaac
     1261 cgtggcgatg aggcccttca aaagggcccg gccagagagg ctgatgacca ggctcaggct
     1321 catgaacatc agtgcaaaat attcgggcgg accgaaggcc agggccacgt tggccaggat
     1381 cggcgcaaag aaggtgagac ccaccaggct cagcgttccg gccacgaagg aagcaatggc
     1441 cgcgatcccc agcgccggtc cggcccgacc ttgcttggcc agctcgtagc catccagaca
     1501 ggtgggcacg gatgaggcct cgcccggaat gttcaccacg atggccgtgg tggaccctcc
     1561 gtacatggcg ccatagtaga tcgcggccat catgatgatg gcggggatcg gccccaccac
     1621 cgtggtgagc ggcaacagga tggcaatccc agaagagggg ccgattcccg ggagcacgcc
     1681 gatcagggtc cccatgaggc agcctaccag cgcccagagg agatttacgg gggacaaggc
     1741 cattgagaac ccttggagca aatggtccca ggagaagagc atggcgaccc ccctatcgcc
     1801 ctagaggccc ggcggggaat gacaattcga gaagacggat gaacacgtag tacaccgtcg
     1861 cgccggaaac caaccccagg gcggcggccc atatcgcgcg gtagcgtccc aggtaccaca
     1921 ccagaaaggc gacgaacaac acggtgccca gcgggaggcc aaggggtatc atcaggatca
     1981 ggtaggccgt caggctcagc agggttaccg cgatacgccg tgtcccagcg cggtttggcc
     2041 acttgatgga tctgtcttcc ccttggaagt gccaggcatg cacggccagg atcgccccgg
     2101 ccaccaggat ggtccagccc aggatcatcg gcagggtccg gggctccagg cgcacgtcgg
     2161 gagagccggc gatcctccgg gccgccagga tgacgagcag tcccaggaga gacaagaaga
     2221 gcccgctggc gaaatccgct ccgcgctgtg ttctcacgct cggaccccct cggctctatt
     2281 tcttgatacc tgccatcagc tcccggtaga tgccagccat cttctccacg taggccttgg
     2341 tctccttgtg tcccatggcc aagggcacga agccctgttt tttcatctcg acctggattt
     2401 ccgggttgcg gcagatttcc agaaaggcgg cctccagtct ctggatgatg gcgtcgggcg
     2461 ttccggccgg aacccccact ccgcggtcca ccgcctcctg gaggtcgata ccctgctcct
     2521 tgaaggtcgg ggcgtccgga aagtcgggga agcgcgcttc gctggcgaag gcgagcacgc
     2581 ggaccttgtc cttgtatcga accaggtcat ccgaattgct gatggcggcc gcagtatgtc
     2641 cacccagaaa agccatggtc tgcggcgccg ctccggtgaa ggggacgtag gtcaccttgg
     2701 ttcccgtgag cttctcgaag cgaagggtcg tcacgtgggg gccggaaaaa gtcgcggacc
     2761 ctccaacggt cagcgcaccg gggttcttct tggcgtagtc gatgaagtcc tgcaacgtct
     2821 tgaactggct ggtattcagg actgcgagcg caaggatggt ccgctggaag agtgccacgg
     2881 gtacgatctg ctcggtcttg tagcccacct cctgctgcat gggctgcagg atgatgtggg
     2941 ggacgttgaa gccccctatg gtgtacccgt cgggcttgga cttcaccagt tcccgccagc
     3001 ccagggcgcc gccgcccccc accttgtagt cgatgaggac cttctgcccg aggagccgct
     3061 cgaggtgggg ctgctgtcgc cgcgcttctc ggtcggactg tccgccgggg tcaaaggtca
     3121 ccaggtaggt gatcgtcttc gccggatact tctcctgggc ctctgcctgg gaaccggcgg
     3181 ggccgattcc caggggcagg gtcattgcaa ggatcgcgat ccacgggaga cgaatgaggg
     3241 acctcggcgt gcttctgggc gtgcgcatgc tgcacctccc ggccccagtg ggccatgact
     3301 ccccagacga ttgctgcaag cttctccgga ctcgaccgat ccggccctca agcggcgccc
     3361 gttgatcagg gcccgaatcc gttctgcgat gcggctatgg gaattgctat cttgcctgcg
     3421 agccggtgag aacactcgca cggcgaccct aggctcccgg atagagcttg gtcaggacga
     3481 actcgcggtg tcccagggcc tcggcggccg tgaggcggcc gttagcactt cacacaatcg
     3541 gctggatgag cctgtccccg tccactccgg ctcgatgccg atcaccacga cggctgccac
     3601 catggagttg gcccctgtgg aggcaggcgg tgagccaaga ggtcatgcct ctcataacct
     3661 atctcccgcg gcggtgctgt caaggccaaa agggggcgtc ggtgccgttt tccgtgcagc
     3721 ggaccggcga gactgtgggg atggatgagg tcgcgaatcc atccgagaat cggatccggc
     3781 ggtgcggctg aagggcaagg tctgtctcgg cgtgtatcac ctctccgctg aaggatgggg
     3841 aagcgagtgc tagccccgca tggccaagac cagcaggaaa aggccgaggg cgaccagcgt
     3901 agatgcagcc aggcgccggc cgccgaagcc ctcctttagc acccgccaac cgaggagcgt
     3961 ggcgatgatg accgagctct cccgcagggc ggcgacgtaa ctcacctgcg tcagtcgcat
     4021 ggcaatcaga accagcaggt aggcgccgaa actcaggagg ccgcccgcca ccaagatcct
     4081 ccaacgcagc cgccagatgg ctcgcagttg ctggaactcc cgctgttgca ggacgtacgg
     4141 cgtcatgatg acagccgtca atccgaagag ggtgatgatg tacgcaagcg gatgcgaccg
     4201 cgagaccccc gccttgtcca caatggaata ggaggcgata agggccgcgg tggtcacggc
     4261 ccagagcagt gcggcgacac gtgtcgcgga tcgtccggct gagaagccga gccaggagat
     4321 tccgccgccg accaggagga tggccagcat ggcaagcggg gacagacgct cgccgagagc
     4381 gataggggcg gccatggcca cgaagaccgg cgccaaaccc cgggccacgg ggtagacaac
     4441 ggacagatcc ccgtatgtgt aggctcgggt cagggctacg acgtacagag attcgagggc
     4501 gccacttccc aaaaggaaag gccaggcctc gcgtggcggt aggccggtga cgaagagagc
     4561 cggggagagg atcaggctcg gcgcgaggac tgtgagccag ccggagaaaa gcttgtcgac
     4621 ctcgctcttg acgatgaggt tccaactggc gtggaggccg gcggagcaca ggacgagggc
     4681 aagggggaga agccacatgc agagtcccct ctggagtccg gttcggcccg gtgcgatggt
     4741 gccaagcggg tcccagcttg gtttctctcc ccggatggac gggggccagg accgccaacc
     4801 tcggggtcgg ccctcggccc tgccttcatc caagagctag acaggaccgg gtctgagtcg
     4861 ccatggatgt gatccagtgc caggcgcggt ctctatacca caggaatcgt gccgtggaca
     4921 agccgacctg ggccagactg gtcgtgtccc ggcttcaggc cgctcggcgg ggagccggag
     4981 ggctcacccc catctggggc gacgacgggg tctcgcgaac cagcactttc tccttgacga
     5041 gcccacgccg ttcggctaga aagtcgacat tcgacattcg gtatttcccg cgatttcccc
     5101 tgactgttca aggcttccca tggcggtccc tgccggaagg cgccgaatct ctccgctcgg
     5161 gcactcgccc ggcatcgccc ccattgcgta ccggactatc gccgagcagg tggccggctc
     5221 gatccgtgag gccattgccc aggggtcgat ccctcccgcc acccggctgc tggaggcgcc
     5281 tcttgcccgg gagatgggga ccagccgggc ccccgtccgg gaagcgctca gtcagttgga
     5341 gcgggagggg tgggttgtca agaagccgaa ccgcggggca cgggtcctgg aattgaccga
     5401 ggagaccatc cgcgaggtgg cgagccttcg cggggtccta gaggggtttg ccgcttccct
     5461 ggcggtggac cggctgaccg ccaaggactt caaggcgctg gaggatggtc tggcggagat
     5521 ggatcgggca gcgcatagag gccagtttcc gcgtctcctg gagttggact tccagtttca
     5581 tgccttcatc tgccaagcct ccggtcatcg gatcctgtac cagacgtgga acgggatggc
     5641 cggcaagatc cggctctttc tctcggcgac gaacctgatg taccaggact tgagccgcat
     5701 cgtccggggt cacaagctga tcctggaggc cttgaagtct cgggacaagg ccaaggcttg
     5761 ccgagtcatg ggggagcatc tgggcgagat gctcgatccg ttcattgcca aactcgtatc
     5821 ctcgcgcgcc cgcgcctacc ggggacgcgc gaggaacctg caggtgcatg cggggcaagg
     5881 ggggtagggg acgcccctgt tcggggggaa gaatcgtgcg acaccggcgc cgcagtcccg
     5941 cgacgccaca caccaaggag gaggccatga agaggagcag gttgtggatg ggggtgctgg
     6001 ccgtcggttt ggtgatgacc ggcttggcct ggtcggtccc ggttcaggca gccgagctac
     6061 aggacacgct gaatgcggtc atcaagaagg gagagttgac cgtcgggacc ttcgacttct
     6121 tcccgccctg ggggttccgg gatgcccaga acaacatcgt gggcatggac gtggacatcg
     6181 ccaaggagtt ggcgggggat gacgtgctga aggtgaagtt gaccctcgtc ccggtgccca
     6241 ccacccagga ccggatcaac ttcctgctgt cgggcaagat cgatgtggcc atctccaact
     6301 tcacggcgac cctgccccgg tccaaggcga taaccttcac cgatccgtat gtgatcgatg
     6361 gcgtcggtgc gatcttccgc aaagaacaca agatcgctga ctggaaggac ctgaacggca
     6421 agcgggtggc ggtgaccaca ggctcaaccg gagagatcct ggtcaagaag caggcgcccc
     6481 aggcgaagct cctccagttc gatcaggcat ccacggcgct cctggccgtc cagcagaaca
     6541 aggccgacgc ccagatcgag gattacacct acacggtgta ccatgccgga cgcaacaagg
     6601 acctcgccta cctgaacaag ccgctggact tccagcccta ctgcttcggc gtgcggaagt
     6661 tcgaccagga atggctgaac tacctgaact tcttcatcta ccggctgcaa cagagtggca
     6721 agatgaagga gctgtacaag aagtggttca atgcggagcc gttccacgtc tcgccgacct
     6781 ggtagagaac tgacggggcg ccccgcggtc tgcaccgggg gcgccccacg gcgccaaggc
     6841 tgacacgcga tgcatggata cgtttttcag tttcgcccca tcttccagga actcccgctt
     6901 ctactcagcg gggccctggt gaccatccat atctcgatcc tgacgctcct tttcagcttc
     6961 atcgtcggca cgatcggggc gctgtgtcgc ctctcgcgct ttcgatggct gaatgtcccg
     7021 gccaggtggt acgtcgagtt catccggaat accccgctgc tcgtccagct cttcttcatc
     7081 tatttcggct tcagccagat caatatcgaa gtgtcgaatt acatggccgg tctcatcggt
     7141 ctcgtggtga acaacggggc ctacctcacc gagatcgtgc gggcgggaat cgaggccatc
     7201 cacaagggac aggtcgaggc gggtctgtcc ttggggctca gctttcccca gctgatgcga
     7261 tacgtggtct tcccgcaggc gttccgggtc atctacccac cgctctgcaa tcagttcatc
     7321 ggcatcatcc tctggtcgtc cctggtctcg acgatcgccg tgcaggatct cgccatgcgc
     7381 gggaaagagc tggcctccga gaccttccgt tctttcgaga cgtatgcggt ggtgaccctg
     7441 gtgtatgtgg tcatgacttt gacgatttct ggctgcctga aggtcctcgg ccggcgactc
     7501 ttccgccggc cggcgtgagg tcgcagggcg agtccggtcc gcaggccggt cccgtccgcg
     7561 cagaacgacg atgccccaat atgtgcaaga cagcctaaac ctgtacgatg cggttctctt
     7621 cctccagggg gtctggaaca ccgtcgcgct gtgcgtggtc gggctgggcc tggggacgct
     7681 gatcgccctg gtcgtgggtc tgtcccgggc ctcccatctt cgctggctct cctggccggc
     7741 cggtctgtac atcgagatca tccgggactc acccctgttg atgcagctct atctcgtctt
     7801 tttcgcgctg ccgctcctcg gactccaggt gcccatcttc gtcgccgggg tggccacgtt
     7861 gatggtcaac tccagcgcct tcatggcaga gatcgtccgg gccgggatcc aggcggtggc
     7921 caaggaccag tgggatgcgg cccggggact cggcatgggc tacctgcagg ccatgcgctg
     7981 gatcgtcctg ccgcaagcct ttcgcgtcat gattccgcca ggggtgggcc tcctgatcgg
     8041 gctgatcaag gactcctcca tcatctcggt catcagctac ctggagctga cgcgggtggg
     8101 gcagatcctg acggagaaga ccttcctgtc cttccaggtg ttcggcatcg tcgcggcgct
     8161 gtattttctc ctgtgttacc cgctttcccg cctggccctg tatacggaac ggaggttgaa
     8221 ggtcagtggt taaggtcaag gacgtccaca agtacttcgg gcatctgcac gtcctgaagg
     8281 gactcaacct ggaggtggct cggggggagg tcgtggtgat catcgggccc agcggctcgg
     8341 gcaagagcac cctgctccgc tgcctgaact acctggagcc ggtgcagtcc ggcgagatct
     8401 ggatcgacgg gattcgtttg gaccatcaca agaccaatat ccgacaagtc cgtgccgagg
     8461 tcggcatcgt gttccaggcc ttcaacctct ttcctcacct cacggccatg cggaatgtca
     8521 tgctcgcccc cctggtggtg aggaaaaggc gtgaggacga ggtccggcga gaggccgtga
     8581 ccctcatgac caaggtgggc ctgcaggaca agatcgacgc ctacccagcc cagctctcgg
     8641 gcgggcagca gcagcgggtg gccatcgccc gggccctggc catgcagccg aagctcatgt
     8701 tgttcgatga ggtcacctcg gccctggacc cggagctggt tcgggaggtc ctggatgtga
     8761 tgaagcagct cgcccgggac gggatgacga tgatggtggt cacgcacgag atgagcttcg
     8821 cccgggaagt cgccgaccgg gtgatcttca tggacgaggg gcggatcgtc gaggagggga
     8881 cggcggccga cgtgttcgat cggccgagaa gccagcgaac ccaggagttc ctcggcaaga
     8941 tcctgtaatg ggagtcccgg tcgccgacgc tggcggtccg gaatcccgat gggggtgaga
     9001 tgatggtgga cgtcaacgag acgacgagac aggcggtgat cggctgggac ctgagccggg
     9061 tggtcttccg cgatctggag cgcgattatc cggtggtgga gcggggggaa ggcatctatc
     9121 tcttcgatac caagggccga aagtatatcg atgggtccgg gggctcctcc gtcgtcacct
     9181 ccatcggtca cggcaacaag cgggtggccg cggtcatggc gaagcaggcg gagaccatcg
     9241 ccttcagccc cgcccactgc ttcgccaatc aaccccacat cgagctctgc cagaagatcg
     9301 ccgagatcgc gcccaagggg atggggcaca ccttcctgct gtccgggggc tccgaggcca
     9361 ccgagaacgc ggtgaagttc gcccgccagt acgagttcgt gcggaacaac cggggaaagt
     9421 acacggtcat cggccgctgg cagggcttcc acgggaacac cttcggagcc atgacggtga
     9481 gcgggatcac ctaccggcgc cagatctacg ggccgatgat gggccacgcg gtcaagattc
     9541 caccctgcta ttgctaccgc tgtgattggg gtctgacgta cgggaactgt aacctggagt
     9601 gcgcccgcgc cctgaagaag gtcatccggc aggaagggcg ggagaacgtc ctggccttca
     9661 tcgccgagcc ggtggtgggc gcggccctgg gggcggtgcc ggcggtgccg gaatacttcc
     9721 cgctgatccg cgagatatgc gacgagttcg acgtggtctt catcgccgat gaggtcatga
     9781 ccggcttcgg tcggaccggg gccaacttcg gagtggacca ctggcaggtc accccggaaa
     9841 ttatcgccac cgccaagggc atctcggggg ggtacacgcc gctggccgcc gtcttgctgc
     9901 gggacacgat catcgatacc ctccgggcgc accacagcaa cttccggggc ggccatacct
     9961 actgcgggaa tccgctgtcg gcccggatcg ggatggaggt cctggcaatc atccgggagc
    10021 agaacctcat cgagaatagc cgccgcatgg gcctgcgcct cctggagggg ctgaggaagc
    10081 tcctggacca cccgacggtg ggcgatgtcc gcggcaaggg cctcatggcc ggggtggagt
    10141 tcgtccgcaa caagcagacc aaggagccgt tcccggtcga gtaccagttc ggcaagcgga
    10201 tcttcgagga agcctttgcg cgcggcctgg tcgtctttgc cggcagcggc agcgtggacg
    10261 gggtggcggg ggaccatatc ctcctggcgc cccctctcat catcaccgag ggccaggtgg
    10321 acgacttcct cgcgatcctg gacgaggcca tcggggtggt ggagaaagcg ctgcccgcgg
    10381 acctgagagg ctaaaaaagc tcgcatcgtc aagctgtacg gcaattcggt ggttcaggtg
    10441 tacccgatca accggccggc cgaactgccg aacgccccga ggaggtcttt cgatatgagt
    10501 tcccaggtgt accgcgattt caccaagacc tacccggtca tggtccgggg gaagggcgtc
    10561 tgcctgtatg acacgggggg acgggaatac cttgacgcgg tcggcgggat tgccgtggtc
    10621 aacgtgggcc acggcgttcc ggagatcatt accgccatga ccgagcaggc ccagaaggtg
    10681 gccttcgtcc acggcggttc cttcgccaac gagccggccc aggcccttgc cgaggaactg
    10741 gcccagtggg cgccgcgggg attggggcac gtcctcttac tctccggcgg gtccgaggcc
    10801 accgagaccg ccatgaagct ggcccaccag tatcacctgg agcggggcaa gcccggcaag
    10861 taccgcatca tttctcgctg gatcagttac cacggcaaca ccatcggcgc cctgtccatg
    10921 tcgggccgga ccgcctggcg ccgggagttc acaccgtatc tcctgagctt tccgaagatc
    10981 cacccgccat actgttatcg ctgtccctac ggcaggacct accccacgtg cggaatcgcc
    11041 tgcgccgagg acctggagcg ggtgatcacc ctggaggggg ccgacagcat cgccgccttc
    11101 atcgccgagc cggtcatcgg aacctcggcg gcaggggtca cgccgcctcc cgagtactac
    11161 ccgcgcatcc gcgagatctg cgacaagcac gatgtcctgt tcatcgccga cgaggtgatc
    11221 aacggcatcg ggcgcacggg gaagaacttc ggaatcgagc actggggggt catccccgac
    11281 atgatcacga ccgccaaggc cctgagcagc ggctacgccc ccctggcggc tgtgctcctg
    11341 cacgacaagg tgtacgatgc catcgcccgg gggtcgggcc acacggcgca aggattcacc
    11401 tactccggac acccgttgtg ctccgccgtc ggcttggccg tcctccggta tctgcgggcc
    11461 cacaagctgg tcgagcacgc cgcccggatc ggccatcggt tgctccagcg tctggagtcc
    11521 ctgaagcggt tttccatcgt cggcgacgtg cgcgggaccg gcctgatcct gggcgtggaa
    11581 ttcgtcgccg accaggccac caagcgcccc ttcccaccgg aggcgggcgt cacccggcgt
    11641 atcgtgcagc acaccctggc ggagggcgtc atggtggtgc ccggcatggg cgggatgatc
    11701 gacggagttt ccggggacca catccagatc agcccgccct acatcttcga cgaggccaac
    11761 gtggagcagc tcgtgacggc cctggaggtg gccatccaga aggtcatgca cgaggtgccc
    11821 gtccgatgag gccagggggt gcagcatgaa cagtcacggg atgcacgaga cggggagcga
    11881 gccgtacttc tatagcaact acacctggag ggaacttcga gaggttcccg cggagcggcg
    11941 ggtggtgctg ctccccgtgg ggagcacgga ggatcacggc caccacctgc ccctggacac
    12001 ggacaactac ctggcctgga ccgtctgcgt cgagaccgcc aagcgcatcc cggacgaagt
    12061 cctggtcatg ccgctcatcc cctacggcta caacctgcat cacatcgact ttccggggac
    12121 catctgcgtg gcggcggagc atttcatcaa ctacgtctcc gacgtgtgcc agagcgtcgc
    12181 ctaccacggg ttcaagcgca tcctgatcgt ggacggccac gggtccaacg tgccgctcct
    12241 ggacgtggtg gcccgccggg tgatcctgca gaccgacgcc ctgtgcgcct ccttcattta
    12301 caccaacctc atcaaggacg tgtgccgcag gatccgccaa tcggtgcacc ccggtgggat
    12361 ggcgcacgcc tgtgagctgg agacgtcgct tttcctgtac ctgaacgagc agcgggtgcg
    12421 gaaggcggag atccagaaag aggtcaattt gcctcccagc aagtacatct ggctggatct
    12481 cgtggacgga tcgccccttc agctgatgga ctggtggagt accttctcca agacgggggt
    12541 cgtcggcgat ccgaccctgg caacccggga gaaaggcgag cagatgttca cggcggtgac
    12601 cgagcggatg gtcgagctgg tgcgggagtt cagggaccgc ccgcgagagc cgcgacacga
    12661 catgcacgcc gagacgccgg gagatctctt cggcctgcat ccctaggtcg ggagagcggc
    12721 gattccccgg ttgtgggggt agcagccttc acctgaaaga gaaggagaac gtcatgacca
    12781 cgcagaacga cgcccgcttc gcccgagagg aatacctgcg gcacatcatg ctggacttcc
    12841 gccagatgac ggacttcgtg aaggagccgt tcctcgtggc gaaggccgac gggatccgct
    12901 tctgggacgt acagggcaag gagtacctgg atggtctcgc ggggatcttc gtggtcaacg
    12961 tgggatacaa caatccccgg gtgctggaag cggtgcgtcg ccaactggag acgctgccct
    13021 tcaatccccc catgcacggg accaatcc
//
LOCUS       DBNGZC010000642         2611 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2307143, whole genome shotgun sequence.
ACCESSION   DBNGZC010000642 DBNGZC010000000
VERSION     DBNGZC010000642.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2307143"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /gene="nusA"
                     /locus_tag="AC16G0_11195"
     CDS             complement(<1..1374)
                     /gene="nusA"
                     /locus_tag="AC16G0_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR5100213.1"
                     /translation="MDLMQVIDQIGREKGIEKGILIEAVGAAILSASRKSLPVAQAAL
                     DLRVDFDGKTGQFMLYAVKKVVEAPTNSKIEVSLEEALQINPEAKVGDEVKTPVPLVA
                     GSFGRIAAQTAKQVIIQRVREAERDSVFQTFKEKEGELVHGVVQRVIKGNAILNIGKA
                     EAILPVREQLPREEFRVGDRVRAYILDVKKTSKGSQIVVSRTHPGFVKRLFELEVPEI
                     YEGIVEVRAAAREAGERAKIAVVSKDNNVDPVGACVGYRGARVQAIVRELQGERIDII
                     PWREDPAAFVKAALAPAEVQSVSAESDTRTLRVVVADDQLSLAIGKRGQNARLAAKLV
                     GWKVDIKSMAELQREAEVTLTGLMGGETKPAATPPGPAETGVVLEQLTALPGVGEKLA
                     GRLVAAGFAGLTALADATPEALQAIEGIGPKSAERILLAVREALANPSGVAPAPEEPA
                     AAENAVVQ"
     gene            complement(1450..1941)
                     /locus_tag="AC16G0_11200"
     CDS             complement(1450..1941)
                     /locus_tag="AC16G0_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIR5100214.1"
                     /translation="MPTGGAGASGAATEVVARVRALAEPILADLGLELIDVEFRREAQ
                     GWVLRVYMDRAGGVSLHDCQRVSEELGDHLDVEDVIDHPYHLEVSSPGLDRPLTRDAD
                     FLTFAGKEARITTREAVGGQKNFRGRLAGLTQGAVLLDLADGRRVEIPRGLIARARLQ
                     PEF"
     gene            complement(2053..>2611)
                     /gene="fusA"
                     /locus_tag="AC16G0_11205"
     CDS             complement(2053..>2611)
                     /gene="fusA"
                     /locus_tag="AC16G0_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547529.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR5100215.1"
                     /translation="PMPRSAGFEFVNAIVGGSIPKNYIPAVEKGVVEAMERGSVAGYP
                     VVDLKITLYDGSTHPVDSSEMAFKLAGRLAFRKAMTQAKPTLLEPVMTVEVVAPGDCM
                     GDIVGDLNSKRGRVLGIESKGRNQAVNANVPLAEMLEYDSRLRSITGDRGDYSMEFSH
                     YEEVPAHIQEKIVAEAKHPVEEEEE"
BASE COUNT          409 a          966 c          789 g          447 t
ORIGIN      
        1 ctggacgacg gcgttttcgg ccgcagcggg ttcctccgga gccggcgcga cgccggaggg
       61 attcgccagc gcctcccgga ccgccagcaa gattcgctcc gcgctctttg gtccgattcc
      121 ctcgatcgcc tggagcgcct ccggggtggc gtcggccagg gccgtgagcc cggcgaaacc
      181 agccgccacg agacgccccg ccaacttctc ccccacgccg ggcaaggcgg tcaactgctc
      241 caggaccacc cccgtctccg cgggaccggg aggagtcgcc gcaggcttgg tctcgccccc
      301 catcaggccg gtgagggtga cctccgcctc gcgctgaagc tccgccatgc tcttgatgtc
      361 caccttccag ccaaccagct tggcggccag gcgggcgttc tgccctcgct tgccgatggc
      421 caaggagagc tggtcgtcgg ccaccaccac ccggagcgtt cgcgtgtcgg attcggcgct
      481 cacgctctgc acctcggcgg gggccagggc agccttcaca aaggcggccg gatcctcgcg
      541 ccacgggatg atgtcgatgc gctcgccctg cagctcccgg acgatggcct gcacccgggc
      601 cccccggtac ccgacgcacg cccccaccgg gtccacgttg ttgtccttgg agaccaccgc
      661 gatcttggcg cgctcgccag cctctcgcgc cgccgcccgg acctcgacga ttccctcgta
      721 gatctccggt acctccagct cgaagagacg cttcacaaat cccggatgcg ttcgggacac
      781 gacgatctgg gatcccttgg aggtcttctt cacatccagg atgtaggccc ggacccggtc
      841 acccacgcgg aactcctctc gggggagctg ctcgcgcacg gggaggatgg cctcggcctt
      901 accgatgttc aggatggcat tgcccttgat gacccgctgg accaccccgt ggaccagctc
      961 gccttccttc tccttgaacg tctggaagac cgaatcccgc tccgcctccc ggacccgttg
     1021 gatgatcacc tgcttggccg tctgggccgc gatccggccg aagcttcccg ccaccaacgg
     1081 cacgggcgtc ttgacttcat ccccgacctt ggcctctggg ttgatctgca gcgcttcctc
     1141 caggctgacc tcgatcttgg agttggttgg agcctccacc accttcttga ccgcgtagag
     1201 catgaattgg cccgtcttgc cgtcgaagtc cacccgcaaa tccagggctg cctgcgcgac
     1261 ggggagactc ttgcgggatg ccgacaggat ggccgccccc accgcctcga tgagaatgcc
     1321 cttctcgatg cccttctctc gtccaatctg atcgatgacc tgcatcaggt ccattccgag
     1381 cctcccctgt cgtacccggg gccgaaggca ccccgcgccg cctgacccga ccgcggggcg
     1441 atggcggagc tagaattccg gctgcagccg ggccctggcg atgagcccgc gagggatctc
     1501 tactcgccgg ccgtcggcaa ggtccaagag caccgccccc tgcgtgaggc cggccagacg
     1561 gccgcgaaag ttcttctgac cccccacggc ctcgcgagtc gtgatccgcg cctcttttcc
     1621 ggcgaaggtg agaaagtcgg cgtcccgcgt gagcgggcgg tccagccccg gagacgagac
     1681 ctccagatgg taggggtgat cgatcacatc ctccacgtcc agatggtcac cgagttcctc
     1741 gctgacccgc tggcagtcgt ggaggctcac cccgcccgcg cgatccatgt agacccgcag
     1801 cacccagccc tgcgcctccc ggcggaactc gacgtcgatg agttccagcc cgagatcggc
     1861 caggatgggc tcggccaggg ctcggacgcg ggccaccacc tctgtcgccg ccccggacgc
     1921 ccccgcgccc ccggtaggca tcaaccgaca cccctcgacc cgtgaaaaac aaagagtggg
     1981 caggtgccca ctcgatgcgg caaagcatac cactccggcc agatcggacg caagggaaaa
     2041 atcgcggtgg ggctactctt cctcctcctc gacggggtgc ttggcctcgg ccacgatctt
     2101 ctcctggata tgcgccggca cctcctcgta atgggagaac tccatgctgt agtccccacg
     2161 atcacccgtg atggaccgca gtcgggagtc gtactccagc atctccgcca gagggacgtt
     2221 ggcattcacc gcctggttgc gccccttgct ctcgatgccc aggacccgac ctcgcttgct
     2281 gttcagatcc ccgacgatgt ctcccatgca atccccgggg gcgaccacct ccacggtcat
     2341 cacgggttcc aggagcgtgg gcttggcctg ggtcatggcc ttgcggaaag cgagccggcc
     2401 cgccagcttg aaggccatct cggaagagtc tacgggatgg gtggagccgt cgtacagcgt
     2461 gatcttgagg tccaccacgg ggtaaccggc cacggagccc cgctccatgg cctccaccac
     2521 gcccttctcc accgccggga tgtagttctt ggggatggag ccgcccacga tggcgttcac
     2581 aaattcgaag cccgcgctcc gcggcatcgg c
//
LOCUS       DBNGZC010000643         5380 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2499156, whole genome shotgun sequence.
ACCESSION   DBNGZC010000643 DBNGZC010000000
VERSION     DBNGZC010000643.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5380)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2499156"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1055
                     /locus_tag="AC16G0_11210"
     CDS             <1..1055
                     /locus_tag="AC16G0_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIR5100216.1"
                     /translation="LLIFEQALVESVYAAQPPVEFTAAILAAGMREGHSGPIFLQADH
                     DQVNAKNYQKDAQAEIQRLSDVIREQIAASFFNIDIDASTVVDLSLPTLVDQQRLNAE
                     LTARFTRLVRTLEPKGVTISVGAEIGEVGAHNTTPEELRAFMATYPMALAAAGGGAGI
                     TKVSVNSGTYHGGKVLPDGSLAKVPVAFDTLRDISRVAREEFGLAGAVQHGASTLPMD
                     FFGKFPESGTVEIHLALGFNNLIFDHPKLPQEVKDEIRSYCFAHHASERKPDQTEAQF
                     IYTTRKKAWAGMKERFWNLPGDIQEALMDSLEEMFRDMFVRMHVGQTAGLVAQHTQAP
                     VVPVPIPPALARGLEV"
     gene            1105..2058
                     /locus_tag="AC16G0_11215"
     CDS             1105..2058
                     /locus_tag="AC16G0_11215"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR5100217.1"
                     /translation="MTSVELATRPFDVVGFGVNALDLIAIISGYPQPDTKVQFQEFDV
                     QGGGVVATAVAACARLGLRTRYIGKVGTDFWGRASLRTLSKEGIDVRHVIKAKGSPGH
                     VSLVLADKHSGARTLFFRRPPAYAIRPDELNREALTAGRLLHVDGIDAAAALQAVTWA
                     REAGMVVTMDGERIVEGIEKVWPLADLLVCNPRFVRATTGHAAVEDGLREMADRGPSR
                     LAVTLAEKGVLGFENGQWVRVKGFRVEPVDTNGAGDVFHGACAFGALRGWPFEWVLTF
                     ANAVAAMKCRFLGGRRGIPRLPEVAEFLNTHGRSDIADALI"
     gene            2374..2841
                     /locus_tag="AC16G0_11220"
     CDS             2374..2841
                     /locus_tag="AC16G0_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012976978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5100218.1"
                     /translation="MANVLMIHPDKCTGCRTCELACVFGKEGRFRPRASRVHVFTWDR
                     EGISVPMMCQHCDDAPCVSVCTPGAMHRNAGTGWVEWDTKKCIRCRMCVQACPFGNAV
                     YDGLTGSILKCDACQGNPECVAFCPNKALEFAEDTLSTRSRKKGFAAKFKEAF"
     gene            2858..4663
                     /locus_tag="AC16G0_11225"
     CDS             2858..4663
                     /locus_tag="AC16G0_11225"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR5100219.1"
                     /translation="MHGWIGTILRVNLTDRTVKEEPLDLKLARQFFGARGLGTKILYD
                     EMDPKADPLGPDNKLIFAPGPFSGTYAPSAGRYNVITKSPLTGAIAASNSGGAFGPEL
                     RYAGYDLLILEGKADKPVYLFIRDKQIEIRDAAHLWGQSVPDTTDRVRAETDDDAKVA
                     CIGPAGERLALLANILNEMHRAAGRGGVGAVMGSKNLKAIAVLGGGGVDVADPNGFRD
                     AVMQARQKIQAHPVGGGGLKAFGTDVLINILNQTGALPTRNFREGQFPTADKVGGESL
                     SAQILVRPKGCFSCIISCGRVTEVKDPKYQGAGEGPEYETAWALGPDCGVDNLNAITK
                     ANYLCNEFGLDPISLGSTIACAMDLYEAGIIGLKDTGGIPLTFGNPEALVEMVRLTGL
                     RQGLGDKLALGSFRLAESFGHPEFSMTVKKQEMPAYDPRGVQGIGLNYATSNRGGCHV
                     RGYTIASEILGIPAKMDNLTTEGKAQLDITFQNLTAALDSTGACLFCLFGIGADELAR
                     MLSSLTGVTCTTEDFLKTGERIWNLERLFNLKAGFTAKDDTLPDRLLKSPIPGGPAKG
                     NVNRLGEMLPEYYHLRGWDKNGVPTEKKLKELALA"
     gene            4809..5081
                     /locus_tag="AC16G0_11230"
     CDS             4809..5081
                     /locus_tag="AC16G0_11230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD family protein"
                     /protein_id="HIR5100220.1"
                     /translation="MQILFFATIRNITGEKEIRWPTPSPTLGDLLRDLSARYGPQFQR
                     WVLEGEDLGGAILVQVNGHDARHEGGLKAPLKPEDIIAIFPAIAGG"
     gene            5103..>5380
                     /locus_tag="AC16G0_11235"
     CDS             5103..>5380
                     /locus_tag="AC16G0_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIR5100221.1"
                     /translation="MEQLAALPLAALLAGVLLQLLLGRILSASGKGWLAVISGLMALI
                     GAATLVPSIARGTAIKGRLFLWDDGVPFVLHVDGLSLVFLLMATGIGSA"
BASE COUNT          944 a         1701 c         1820 g          915 t
ORIGIN      
        1 gcctccttat cttcgaacaa gcgctggtgg aatcggtcta tgcggcccag cctcccgtcg
       61 aattcacggc ggccatcctg gctgcgggaa tgcgggaggg gcactcgggc ccgatctttc
      121 tccaggcgga tcacgatcag gttaatgcca agaactacca gaaggacgca caggcggaga
      181 tccagcggct cagcgacgtg atccgggagc agatcgcggc tagcttcttc aacatcgaca
      241 tcgacgcctc tacggtggtg gatttgtcct tgccgacgct cgtggaccag cagcggctga
      301 acgccgagct cacggcgcgg ttcacccgcc tggtgcggac gctcgagccc aagggcgtca
      361 ccatctcggt cggcgccgag atcggcgagg tgggggccca caacacgacg ccggaggaac
      421 tgcgcgcctt catggccacc tatcccatgg cgctggccgc cgccgggggc ggggcgggca
      481 tcaccaaggt cagcgtgaac tcggggacct accacggcgg taaggtcctg ccggatggaa
      541 gtctcgccaa ggtgccggtg gccttcgaca cgctccgaga catctctcgg gtcgcacgcg
      601 aagaattcgg tctggccggg gcagtgcagc acggcgcctc cacgcttccc atggacttct
      661 tcggcaagtt tccggagagt ggcacggtgg agatccacct ggccctgggc ttcaataatc
      721 tcatctttga tcatccgaag ctgccgcagg aggtgaagga cgagatccgg tcctattgct
      781 tcgcgcacca cgcgagtgag cggaagcccg accagactga agcccagttc atctacacca
      841 cccggaagaa ggcgtgggcc ggcatgaagg agcggttctg gaacctaccc ggcgacatcc
      901 aggaggccct catggactcg ctcgaagaga tgttccgcga catgttcgtg cgcatgcatg
      961 tgggccagac tgccgggctg gtcgcccagc acacccaggc cccggtggtg ccggtgccga
     1021 tcccaccggc cctggcaagg gggctggaag tctaggctgc gcttctggag agtcggcctc
     1081 catgacggcg ggggcgatca agtcatgaca tcggtagaac tggcgacccg ccccttcgat
     1141 gtcgtcgggt ttggcgtgaa cgccctcgac ctcatcgcca tcatcagcgg ctatcctcag
     1201 ccggacacca aggtgcaatt ccaggagttc gatgtccagg ggggcggcgt cgtggccacg
     1261 gccgtggcgg cctgcgcgcg gctcggtctc cgtacccgct acatcggcaa ggtcgggacc
     1321 gacttctggg ggcgggcctc cctccgcacg ctgtccaagg aaggtatcga tgtccgtcac
     1381 gtcatcaagg caaagggaag ccccggccat gtcagcctgg tcctggccga caagcactcg
     1441 ggggcgcgga cgctcttctt ccgtcgccca ccggcctacg ccatccgacc ggacgagttg
     1501 aaccgggagg cgctgacggc ggggcggctc ctgcatgtgg atgggatcga cgcggcggcg
     1561 gccctccaag ctgtaacctg ggcccgcgag gcagggatgg ttgtcaccat ggacggcgag
     1621 cggatcgtcg agggcatcga gaaggtctgg cccctggcgg accttctggt ctgcaacccc
     1681 aggttcgttc gggcgaccac cggacatgcg gcggtggaag acggcctccg cgagatggcg
     1741 gatcggggtc catcccgcct ggccgtcacg ctggccgaga agggagtcct tgggtttgag
     1801 aatggccagt gggtcagggt aaaggggttc cgggtcgagc cggtggacac caacggtgcg
     1861 ggagacgtgt ttcatggggc ctgcgccttt ggcgcgctgc ggggttggcc gttcgagtgg
     1921 gtcctgacct ttgccaacgc cgtggctgcc atgaaatgcc gcttcctggg aggtcgccgc
     1981 ggcatcccga ggctccccga ggtggctgag ttcctcaaca cgcacggccg gtccgacatc
     2041 gcggatgcct tgatctgacc ccacctgcca atggccaggc cggctgaagg tcctgcatac
     2101 tggacggaag cccctttcat cttgacattc gcctccgagg agggctagct tcagacaaca
     2161 aggatcagtt ctccctgact tgagggggag gggagagcgg tgccggccgg tgggggacac
     2221 ccatgggcgc agggacggct cttctgatgg acggtagaac aatcggcggc ccttcgggga
     2281 tgtggacacc cggagggccg tcgtgttttg tggtcaggct gccgccttct tcctgagacc
     2341 cccgtcaccc ttccgtagag aaaggaggat gcgatggcaa atgttctcat gatccacccg
     2401 gataagtgca ccgggtgccg aacctgcgag ttggcctgcg tctttggcaa ggagggccgc
     2461 ttccgacccc gggcgagccg ggtgcatgtg ttcacctggg atcgggaggg catctcggtt
     2521 cccatgatgt gccagcattg tgatgatgcc ccgtgcgtgt cagtgtgcac tccgggggcc
     2581 atgcaccgga atgcgggcac gggatgggtg gaatgggaca cgaagaagtg catccgatgt
     2641 cgcatgtgcg ttcaggcctg cccattcggg aatgccgtgt acgacgggct gacggggagc
     2701 atcctgaagt gcgatgcttg ccagggcaat cccgaatgcg tcgccttctg tccgaacaag
     2761 gccctggagt ttgccgagga cacgctctcg acgcggtcgc gcaagaaggg tttcgcggcc
     2821 aagttcaagg aagctttcta aagaggaggc tggagccatg catggctgga tagggaccat
     2881 cctgcgggtg aatctgaccg atcgcacggt gaaggaggag cccctggatc tcaagctggc
     2941 ccgccagttc ttcggcgccc gggggctcgg gacgaaaatc ctgtatgatg agatggaccc
     3001 caaggcggat ccgctgggcc cggacaacaa gctgatcttc gctcccggac cgttctccgg
     3061 cacgtacgcc ccctccgctg gacgctacaa tgtcatcacc aagagcccgc tcaccggagc
     3121 gatcgcggcg tccaactccg gtggggcctt cgggccggag ctcaggtacg cgggctatga
     3181 cctcctcatc ctggagggca aggccgacaa gccggtctat ctcttcatcc gagacaagca
     3241 gatcgagatc cgcgatgccg ctcatctctg gggccagtcg gtgccggaca ccaccgatcg
     3301 agtgcgcgcc gagaccgacg acgacgccaa ggtggcctgc atcggcccgg ccggagagcg
     3361 attggccctc ctggcgaaca tcctgaatga gatgcatcgg gccgccggtc gaggcggcgt
     3421 gggcgcggtg atggggtcca agaacctcaa ggccatcgcc gtcctgggcg ggggcggcgt
     3481 ggatgtggcg gaccccaacg ggttccggga cgccgtgatg caggcccggc agaagatcca
     3541 ggcgcacccg gtggggggcg gaggcctcaa ggccttcggc accgacgtgc tgatcaacat
     3601 cctgaaccag accggcgccc tcccgacgcg caatttccgg gaggggcagt tcccgacggc
     3661 cgacaaggtg ggcggcgagt cgctgagcgc ccagatcctg gtccggccca agggctgctt
     3721 ctcctgcatc atcagttgcg ggcgcgtgac cgaggtgaag gatccgaagt accagggggc
     3781 gggcgagggt cccgagtatg aaacggcctg ggccctgggc ccggactgcg gggtggacaa
     3841 cctgaacgcc atcaccaagg ccaactacct ctgcaacgag ttcggcctgg atcccatcag
     3901 cctgggttcc accatcgcct gcgccatgga cctctacgag gcggggatca tcggcctcaa
     3961 ggacaccggc ggcatccccc tcaccttcgg gaatccggag gccctggtgg agatggtacg
     4021 actaacgggc ctgcgccagg gccttggcga caagctcgcc ctggggtcgt tccgcctagc
     4081 cgagagcttc ggccacccgg agttctccat gacggtcaag aagcaggaga tgccggccta
     4141 cgatccccgg ggggtgcagg gcattggcct gaactacgcc accagcaacc ggggcggctg
     4201 ccacgtgcgc ggttatacca tagcgtccga gatcctgggc atccccgcga agatggataa
     4261 cttgacgacc gagggcaagg cccaactgga tatcaccttc cagaatctga ccgccgccct
     4321 ggattcgacc ggcgcgtgcc tcttctgcct cttcggcatc ggggccgatg agctggcccg
     4381 gatgctcagc agcctcaccg gtgtgacctg caccaccgag gacttcctga agaccggcga
     4441 gcgcatctgg aatctggagc ggttgttcaa cctcaaggcc ggtttcacgg cgaaggacga
     4501 taccctgccg gaccgcctgc tcaagagtcc aattccaggc gggcccgcca aggggaacgt
     4561 caaccggctg ggggagatgc ttcccgagta ctaccacctg cgcggatggg acaagaacgg
     4621 ggttccgacc gagaagaagc tgaaggagct ggccctggcg tgaagacgcc cgcccagcgg
     4681 tgcgggcgct ggaagtcaat tattcaaggt gtgaaggccc tctgggtcgg ccgtgcggcc
     4741 gtgcttcagc cgtatcggct gacccggggc gcccgggaca cccgcgtccc aaggggacga
     4801 cacgcaccgt gcagatcctc ttcttcgcca ccattcgtaa catcaccgga gaaaaagaga
     4861 ttcgctggcc cacgccgtcc cccaccctgg gtgacctgct gcgggatctc tcggcccgct
     4921 acggcccgca gtttcagcga tgggtgctcg agggagagga cctgggtggg gccattctcg
     4981 tccaggtcaa cgggcacgac gcgcggcatg agggcggcct caaggcgccg ctcaagccgg
     5041 aggatatcat cgcgatcttc ccggccatcg cgggtgggta aggcgttcga attggaggcg
     5101 tggtggaaca actggctgcc ctcccgctag ctgcgctcct ggccggcgtg ctcctgcaac
     5161 tcctcttggg caggattctc tcggcgtcgg gaaagggatg gctggccgtc atcagcggcc
     5221 tcatggctct catcggcgcc gccacgctcg taccctccat cgctcgcggg acagccatca
     5281 agggcaggct cttcctctgg gacgacgggg tgccgtttgt cctccacgtg gacggcctga
     5341 gcctggtctt cctcctcatg gccaccggca tcgggtcggc
//
LOCUS       DBNGZC010000644         1978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2499205, whole genome shotgun sequence.
ACCESSION   DBNGZC010000644 DBNGZC010000000
VERSION     DBNGZC010000644.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2499205"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /gene="istB"
                     /locus_tag="AC16G0_11240"
     CDS             <1..700
                     /gene="istB"
                     /locus_tag="AC16G0_11240"
                     /inference="COORDINATES: protein motif:HMM:NF013828.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HIR5100222.1"
                     /translation="HAAEALVAELTEAAQHDRAAHQVLDRLLTLELSQRDERRIRTSL
                     RLSGLPPGMTLGEFDFAFQPSVERRQIETLATCTFIREHATLLIQGPPGVGKTHLCVG
                     LGVKAIEHGFSVAFYILEDLLHEMRKDAAVAPQRLRRKKYFNVSYLVVDEVGFEPMSR
                     EDASLFFRLVSYRYCRGSTAITTNKSVKDWPGIFAGEEALAAAILDRLLHRSVVLNIR
                     GRSDRLQELEKLLK"
     gene            973..1934
                     /locus_tag="AC16G0_11245"
                     /pseudo
     CDS             973..1934
                     /locus_tag="AC16G0_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070789.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT          370 a          595 c          660 g          353 t
ORIGIN      
        1 ccacgccgcg gaggcactgg tcgcggagct aaccgaagcg gcccagcacg accgcgcggc
       61 gcaccaggtc ctggaccggc tgctgacgtt ggagctgagc cagcgtgatg agcggcggat
      121 ccggacctcg ctgcggctct ccgggctgcc accggggatg accctgggcg agttcgactt
      181 cgcgttccag ccgtcggtcg agcggcgtca gatcgagacc ctggcgacct gcacgttcat
      241 ccgggagcac gcgacgctgt tgatccaggg gcccccggga gtcgggaaga cacatttatg
      301 tgttgggttg ggagtcaagg ccattgagca tggcttctcg gtggcgttct acatcctgga
      361 ggacctgctg cacgagatgc gcaaggacgc ggccgtggcg ccgcagcggc tgcggcgcaa
      421 gaagtacttc aacgtctcgt acctggtggt cgacgaggtc ggcttcgagc cgatgagccg
      481 cgaggatgcc agcctgttct tccggctggt gagctaccgg tactgccgtg ggagcacggc
      541 gatcacgacg aacaagagcg tgaaggattg gccggggatc ttcgccgggg aggaggcgct
      601 ggcggcggcg atcctggatc gcctgctgca ccggagtgtg gtcctgaaca tccgtggccg
      661 gagcgaccgg cttcaggagc tcgagaagtt gctgaagtga ccggcccagc ccagcggccc
      721 ctcggggccg ccttggcggc cggatttcgg ccccctcggg ggccgaatgg gaagacaaac
      781 gtcgcccggc cggctcgtaa aagatcgtgt cactcaacct cgtaagagtt ggtgtcactt
      841 gacaattgcg ggaggctcat ccgggattcg ggggtggtaa taccagcgcc gcctctacgg
      901 ggataagatc aacgttttgg acagattaca tccaccccct gctgcagccc ctgacccgag
      961 ccacccccaa tgatgctcaa catccaggcc ctgatcgacg acgccaagtg cttcgaaacc
     1021 gtccgggcga tgcgatggcc cgatggaatc cgatgccccg agtgcgactg tgccgaggtc
     1081 accaaggacg ggcgggacga cacccagtct gagcggcagc gctaccagtg tcacggttgc
     1141 tggaagcggt tcgatgacct gactggcacc gtcttcgctg ggcaccatca gcccttgcgg
     1201 gtctggatct tgtgcctgta tttcatgggc ctgaacctcc ccaacgagca gatcgcccaa
     1261 gagttggacc tcgaccccga tgatactcag gagatgacct ccgaactccg ggaaggcatc
     1321 gtccggcgta agcccgaggt gaagctcagt ggcgaggtcg aatgcgatga gctctacgtg
     1381 gtggccgggc acaagggcca tcccgaggcc gtcgcaaaaa agggcgagag ggccgccgcc
     1441 gacgcctgaa gggggcaccg ggacggggaa cgctggagaa agagaagccg ccgatctttg
     1501 gcatgatcca acgcaccggc gaggtggtga tccggatgct ggagaatgtt caacaggtga
     1561 ccatcggccc gttgattcgg gcgatgatca caccgggaag tgtggtctac accgacgagt
     1621 acgacatcta cgaccggctc tcggagtggg gctacatcca tcgcacggtc tgtcacgccg
     1681 ccggggagtt cgcccgtgat gatgatgggg acgggttctg cgaggtccac gtcaacaccc
     1741 tggaagggtt ctggtcattg ctgaggtcgt ggctccgccc tcatcgtggg atctcgcagg
     1801 agaagttgcc gatctacttg ggtttcttcg agctcgtgca caacatccga agctcactgt
     1861 tccccaagtt ttattcgctc attgcgggcg gatccaaacc cgtgttcctc ctgcggtggg
     1921 actcgacgcc ttgaaccatg accatcaggc gttgccaacc gcgccagatc gtgatcca
//
LOCUS       DBNGZC010000645         3328 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1917289, whole genome shotgun sequence.
ACCESSION   DBNGZC010000645 DBNGZC010000000
VERSION     DBNGZC010000645.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1917289"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..981)
                     /gene="dapA"
                     /locus_tag="AC16G0_11250"
     CDS             complement(88..981)
                     /gene="dapA"
                     /locus_tag="AC16G0_11250"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR5100223.1"
                     /translation="MGKEIRGIIPAVITPFLPDERVDEAAFRKVLNAMIDQGVHGLFP
                     VGTGGEFSALTTDEKKGLTTVAVKEAGGRVFVMPNVGAITTAESVDLARHAESVGCDA
                     VSAVTPFFLKPSQDELFEHYRAICAAVKIPVLAYNIPERTGGVALATATVSRLAREVP
                     NFAGIKDSTGDLSNAAELVQMCPPGFRVFMGRDTLIFGALLYGCVGAVAATANVAPAL
                     AVGIHAAVEAGDLKKARELQAKLAPVRKLFAVGSHPAGLKEAMVQLGMIECGRCRRPT
                     LDLTSAQKHEVSKVLREAGLL"
     gene            complement(1082..2500)
                     /gene="mtaB"
                     /locus_tag="AC16G0_11255"
     CDS             complement(1082..2500)
                     /gene="mtaB"
                     /locus_tag="AC16G0_11255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035598 - tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HIR5100224.1"
                     /translation="MTECSKMRIAFATVGCRLNQFETDALRGRAQAEACEVVPFDAVA
                     DVYVINTCTITAEADADSRQLARRAVRRNPSALVAVTGCYAQAAPETVASIAGVDLVL
                     GNGEKADLFRYLQTARKNGGATVRVGEIGGRRRIDAYGPGIDPDRTRAFLKIQDGCNY
                     RCSFCIVPQVRGLNRSQQPERVLDQIQRLEGAGFPEVVLTGIHLGTYGRDLAPATSLA
                     DLCRRIAALERAPRIRLSSLDPHEVKPDLIRILADSPRFCRHLHLPLQSGDEGVLRRM
                     RRGHTAAQFRLLVDGLVAEVPGMAVTGDVIVGFPEEDEPAFRNTYDLLDGVPIAGMHV
                     FSYSRRPGTDATGFANQIPKAVKAERSRLLRALVVRKVQAFRRRFIGQTLEVVVLDRD
                     GAGDLLEGLSDNNLRIWFPGNPCLRGTLARVRVDQVTGRGLLGSLFRGEAALDHRQSS
                     LSGGPPGPADAGASQLSAGGMC"
     gene            complement(2536..3030)
                     /locus_tag="AC16G0_11260"
     CDS             complement(2536..3030)
                     /locus_tag="AC16G0_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HIR5100225.1"
                     /translation="MKAYLDIETCAGGQVTVVGVYREDRGLLQLVGGEITDETVWQAL
                     DGVETVCTFNGDRFDLPLLERQTRLDLRSQFRSLDLLRECRRVGLKGGLKRMEERFGI
                     ARTTRGMNGWDALQLWARYEGDGDQEALRLLLEYNREDVMNLVQLERIVVGVGLELEE
                     NRGG"
BASE COUNT          572 a         1185 c          984 g          587 t
ORIGIN      
        1 accagatgct ctgacggacc aagaggcgtt ccccggcgta gcatgcgagg ctcgatgtcg
       61 aacctccaac atggagcttc gaaccgccta caggagtcca gcctcccgta agaccttgct
      121 gacctcatgc ttctgtgccg acgtgaggtc caaggtgggg cgccgacacc gaccgcactc
      181 gatcatcccg agctggacca tggcctcctt gagcccggcc ggatgcgagc ccaccgcgaa
      241 gagtttacga acgggggcca gcttggcctg cagctctcga gccttcttga gatcacccgc
      301 ttccaccgcg gcatggattc ccaccgccaa ggccggcgcc acgttggcgg tcgccgccac
      361 cgctcccacg cagccgtaga ggagcgcccc gaatatgagt gtgtctcgcc ccatgaagac
      421 gcggaagccc ggcgggcaca tctgcaccaa ctcggccgcg ttggataggt cgcccgtgct
      481 gtccttgatc ccggcaaagt tcgggacctc tcgggcgagc ctggagaccg tggcggtggc
      541 cagggccacg ccgcccgtcc gctcggggat gttgtaggcc agcaccggaa tcttcaccgc
      601 cgcgcatatc gcccggtagt gctcgaacag ttcgtcctgg gacggcttca ggaagaacgg
      661 cgtcaccgcc gagacggcgt cgcaccccac gctctcggcg tgccgggcca ggtccacgga
      721 ctccgccgtg gtgatggcgc ccacgttggg catcacgaag acccgccctc ctgcctcttt
      781 caccgcgacc gtcgtcaggc ccttcttctc gtctgtggtc agggcggaaa attccccgcc
      841 cgtacccacc gggaacagac catgaacccc ctggtcgatc atggcgttca ggaccttccg
      901 gaaggccgcc tcgtcaaccc gctcgtccgg caggaacggc gtgatcactg ccgggatgat
      961 tcctcgaatc tccttgccca tgtgcgtctt ctccttgatc ccccgtttcc cttctccgtg
     1021 cttcccctgc gaatccggcc tcggcctccg cggcgaaaac tacttcaccc ctggtgtgct
     1081 gtcaacacat tcccccggcc gagagctgcg aggcacctgc atccgcgggt cctggagggc
     1141 caccactgag ggaagactgc cgatgatcca aagccgcctc cccgcggaac agcgatccga
     1201 gcagccctcg ccccgtaact tggtccaccc gcactcgcgc cagcgttcct cgcaggcatg
     1261 gattgccggg aaaccagatc cgcaggttgt tgtcgctcag gccttccagc aggtcccctg
     1321 ccccgtcgcg atccaggacc acgacctcca gcgtctggcc gatgaaccga cgccggaacg
     1381 cctggacctt gcggaccacc agggcccgga gtaaccggct ccgttccgcc ttcaccgcct
     1441 tggggatctg attcgcaaat ccggtggcat ccgtgccggg ccgccgtgag tagctgaaga
     1501 catgcatccc cgcgatgggc actccatcca gtaagtcata ggtgttccgg aacgccggct
     1561 cgtcctcttc ggggaatccc acaatgacat ccccggtcac cgccataccc ggaacctcgg
     1621 ccaccaggcc atccaccaac agccggaact gcgccgccgt gtggccccgg cgcatccggc
     1681 gaaggactcc ctcatccccg ctctgaagtg ggaggtggag gtggcggcag aatcgcgggg
     1741 aatccgccag aattcggatg aggtccggct tcacctcatg cggatccagc gagctcagtc
     1801 ggatccgggg cgcccgttcg agcgccgcga tccgccggca caaatcggcc agcgaggtgg
     1861 ccggggcaag atcccgcccg taggttccga gatggatgcc cgtcaggacc acctcgggaa
     1921 agcctgcccc ctccagacgc tggatctgat cgagaacccg ctcaggttgc tggctgcggt
     1981 tcaagccgcg aacctgggga acgatgcaga agctacagcg atagttgcat ccgtcctgga
     2041 tcttgaggaa ggcccgcgtt ctatcggggt cgatccccgg accgtaggcg tctatccggc
     2101 gacggccgcc gatctccccc acccggacgg tagcaccccc gttcttcctg gcggtctgga
     2161 ggtatctgaa cagatcggcc ttctcaccgt tccccaggac cagatccacg cccgctatgc
     2221 ttgccacggt ctcgggcgca gcctgcgcgt agcagccggt cactgccacc agcgccgatg
     2281 gattccggcg aacggcgcgt cgcgccaatt gccgggaatc cgcgtccgcc tcggcggtga
     2341 tcgtacaggt gttgatgaca tacacgtccg ccacggcgtc gaaggggacg acctcgcacg
     2401 cctcggcctg cgcccttccc cggagcgcgt ccgtctcgaa ttggtttagc cggcagccca
     2461 ccgtcgcaaa cgcgattcgc atcttggagc actcggtcat ccgcgctcag cctccagcaa
     2521 ggtgtcaggg gcggtttatc cgccccgatt ctcttcgagt tccaggccga ctccgacgac
     2581 gatccgctcg agctgcacca ggttcatcac gtcttctcga ttgtactcca ggagcaatcg
     2641 taaggcctcc tggtcgccgt cgccttcgta ccgcgcccaa agctgcaggg catcccaccc
     2701 gttcatcccc cgggtcgttc gcgcaatgcc gaaccgttct tccatccgct tgaggccgcc
     2761 cttgagcccg acccgccggc actctcgcaa gaggtccagg gaccggaact gggaccggag
     2821 atccagccgc gtctggcgtt ccaggagagg caggtcgaac cgatcgccgt tgaaggtgca
     2881 cacggtctcc accccgtcca gggcctgcca gaccgtctcg tcggtgatct ccccgcccac
     2941 gagctggagc agcccgcggt cctcgcgata aactccgacg accgtcacct ggccgccggc
     3001 gcaggtttcg atgtcgaggt aagccttcat acgccaatcc cgccccatgg ggccggtgcg
     3061 gttgcggccg aggatagcat acggcccctc gatttcaatg gcgaaacagg cctggccccc
     3121 ctccttggag aaaccagaca ggctgagtcg tcctgatgcc tagcgcttga ttgtgtacgt
     3181 gagtgccact ctgccagacc tcttgcgtca ttccgatgag aaccggaatc caggtcttgc
     3241 aagggcttct ggacaccggc ttccaccggg gggaggatac tcggaaatga caatcgcttg
     3301 ctcaattagt cgctagaggg gtgatgtg
//
LOCUS       DBNGZC010000646         1935 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_57245, whole genome shotgun sequence.
ACCESSION   DBNGZC010000646 DBNGZC010000000
VERSION     DBNGZC010000646.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_57245"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="AC16G0_11265"
     CDS             complement(<1..200)
                     /locus_tag="AC16G0_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100226.1"
                     /translation="MARTRDGLGDCSELTYWFAGIRRLIEMCLTLKNCQKIMPSSESW
                     MSALETSSRNETLGMPGERSGRA"
     gene            complement(322..801)
                     /locus_tag="AC16G0_11270"
     CDS             complement(322..801)
                     /locus_tag="AC16G0_11270"
                     /inference="COORDINATES: protein motif:HMM:NF016424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR5100227.1"
                     /translation="MGLLAALRRRRFLTAAERAQITAALAGVDRYTRARIGLFIDADA
                     CDDPEKQAEAMLRQWDLPETERATAVLLYVSAVSRTFAVMGGEEVRRIAPRAFWEIVH
                     RDLCHHFEERRYCDGIFKALAQVAVQLQHHFPPETHPECERLSDATPDRPAKAEPTD"
     gene            1086..>1935
                     /locus_tag="AC16G0_11275"
     CDS             1086..>1935
                     /locus_tag="AC16G0_11275"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HIR5100228.1"
                     /translation="MDIRVVSGDVTRFRGDALVISLFEGIRAPGGTAKTIDGVLGGAV
                     RKLIQSGEVRGKWGEQTLIHTLGKLPVDRVLVAGLGKRGEFTLDRVRVISAEAARHLR
                     KIGARRIGSIVHGTGAGDFNAAHAAQAVIEGTLLGLYRFARYKPHADDNNRREVTSFT
                     LVEGDRMKLRAMVEAVRRVQIVAEATNAARDLVNEPGNTLTPTELARRAREMAQGTGA
                     RCRVLGPRELRRLRCGALLGVARGSQEPPRLIVLEFRGSRRREPHLGLVGKGITFDSG
                     GISIKPA"
BASE COUNT          349 a          582 c          670 g          334 t
ORIGIN      
        1 gcccgcccgg aacgctcccc cggcattcca agcgtttcat tgcgagacga ggtctccaac
       61 gcactcatcc atgattccga cgaaggcatg atcttttggc aattcttgag tgtcaagcac
      121 atttcgatca gacgacgaat cccggcaaac cagtaggtga gctcggagca atcgccaagg
      181 ccgtcgcgcg tccgggccat catgagtcga ccgagtcacc accgcattgc aggaagtgcg
      241 agaatggggg actagaaact gctctgcctt ccgcggtgct ccaatatgag acgttgtctc
      301 aggctcccca tggcagctgg gtcaatctgt cggctcggct ttggctgggc gatcgggtgt
      361 ggcgtccgaa agacgttcac attctgggtg ggtctcgggt gggaagtggt gctggagttg
      421 gacggccacc tgcgccaggg ccttgaagat cccatcgcaa tagcgacgtt cttcaaaatg
      481 gtggcagaga tcgcgatgga cgatctccca gaatgcccgg ggggcgatcc ggcggacttc
      541 ctcgccgccc atcacggcga aggtccggct cacggcggag acgtacagca ggacggccgt
      601 ggcacgctcc gtctcgggaa gatcccactg ccggagcatc gcctccgcct gtttctccgg
      661 atcgtcgcag gcgtcagcgt cgataaacag gccgatcctg gcccgcgtgt atcgatccac
      721 ccccgccagg gcggctgtga tctgtgcgcg ctccgcggcc gtcaggaagc ggcggcggcg
      781 aagggcggcg aggagcccca tggctcaggc gcgcgcccgg aggaccgcgg ggtcatccca
      841 cgtggccatg cgtcgtatcc tcctgctggc gtgtcgaacc gccgacgttc tcacggatct
      901 tcccgataca ttgagactcg aaaggtccag gcttctggat actagcccgg ggccgccccg
      961 gatgtccagc atcggtctgg ctgtgcgcgg tcctggactg gtccgccttt ctgggctata
     1021 actgcggacg gctcttggcc atcggatcct ggtgagtgga gtgaactctc gcaaaggagc
     1081 gtggcgtgga catccgcgtg gtctcgggag atgtgacgag gttccgggga gatgccctgg
     1141 tgattagcct gttcgagggc atcagagcac caggtggcac cgccaagacg atcgatgggg
     1201 tcctcggcgg tgcggtacgg aaattgatcc aatcaggtga ggtgaggggt aagtggggag
     1261 agcagacact gatccacacg ctagggaagc tgccggtgga ccgagtcttg gtggcaggtc
     1321 tagggaagcg cggggaattc acgttggacc gggttcgggt gatctcagcc gaggcggcca
     1381 ggcacctgcg gaagatcggt gcgcgacgca tcggcagcat cgtccacggg accggggccg
     1441 gggacttcaa cgcggcacac gccgcccagg cggtgatcga gggcacgctg ctcggcctct
     1501 accggttcgc gcgttacaag ccacatgcgg acgacaacaa tcggcgggag gttaccagct
     1561 tcaccctggt cgaaggggat cggatgaagc tacgggccat ggtcgaggcg gtccgccgcg
     1621 tacagatcgt ggccgaggcg acgaatgcgg ccagagatct cgtgaacgaa cccgggaaca
     1681 cgcttacccc cacggagctg gcccggcgag cccgggagat ggcccaaggg accggcgcgc
     1741 gctgccgggt cctaggcccg cgagaactcc ggcgactccg ctgcggggcg ctcctcggcg
     1801 tggctcgggg cagccaggag ccgcccaggc tcattgtgct cgagtttcgc gggagtcgac
     1861 gacgcgaacc acaccttgga ctcgtgggga aaggcatcac gttcgattcg ggcgggatct
     1921 ctatcaagcc ggcgg
//
LOCUS       DBNGZC010000647         5860 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1032203, whole genome shotgun sequence.
ACCESSION   DBNGZC010000647 DBNGZC010000000
VERSION     DBNGZC010000647.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5860)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5860)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5860
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1032203"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="AC16G0_11280"
     CDS             <1..603
                     /locus_tag="AC16G0_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100229.1"
                     /translation="RWSAKTLKGEGEYSEHCLAFPFVVYLLGFRQDQFEEMRVYFRPA
                     PLLHEADPLYFSNLWNVQAAESPLARCRACLRGRPEIQERPMAEQAASLVEFFWTAGF
                     NRDIEENCFDRARGRDPRIGSLEAWEAATLADPLFPLSLSWEPVGLSLGEALDHWRRH
                     GDHARRVETAADVADVMYRLHEAPGSESGAGPGTWSGTGR"
     gene            603..1268
                     /locus_tag="AC16G0_11285"
     CDS             603..1268
                     /locus_tag="AC16G0_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100230.1"
                     /translation="MFPVYLKDEGFESPDDPIYYLVTADGLFQVKENPVFRSCVGVRG
                     IRGLQGQKDEVRWKLPPIPGALLQRVVGLFREVLRRYRSEAIVLLIYDLATREYGVVV
                     PEQQVTGGHLSYAPGETPPGRIRVGTIHSHAEADAFHSPLDDWDEEHDDGLHVTVGNL
                     DASVSLSCSLVVDGRRFLLRSRDVFLPAPLDTPEQVWFEGLTHIHTEPPARDSLDGRP
                     SAI"
     gene            1367..2083
                     /locus_tag="AC16G0_11290"
     CDS             1367..2083
                     /locus_tag="AC16G0_11290"
                     /inference="COORDINATES: protein motif:HMM:NF013094.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HIR5100231.1"
                     /translation="MADGYPMRHIKVIGVGGIGCALLPHLARYLQAKGEAARLTLVDG
                     DAFEAQNADRQPFSHLGNKAKVKAVELAQQFEGLSFRAVTEYVTRENVETTIGSGDVV
                     FLGVDNHATRRVVSDRCQRLAEVTLISGGNDYTDGNVQVYLRREDRDVTLPLTRFHPE
                     IADPRDRSPHELSCEELSREAAPQLLFANLAVASAMLNAFYACDVGKLQYGEVYLDVL
                     EGRANPIAREPAPDAQDLGT"
     gene            2891..4165
                     /locus_tag="AC16G0_11295"
     CDS             2891..4165
                     /locus_tag="AC16G0_11295"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5100232.1"
                     /translation="MKRRSNTARGGITRRGFLKTVGVVGGAAAGLGFPAVLRAQQEPI
                     KIGHISPQTGFLAAIGKYSDAGAQLAVDEINEKGGLLGRKVQLINEDEANPGVAAQKA
                     RKLIEKDKVIAVTGIVSSASALAVGDEAQRAKVLFINAGANSDEIRGARCHRYVFSTE
                     GSNTQYVSAIASWLLQQPKLKRWYFLTSDYAFGHDLLKVAQKLLQKAGREQVGSELVP
                     TGSADFSSYLLKVKAAKPDLVFQNLAGSDQTNFLKQFKEFGLDMEVAGGLVDTAVVWP
                     VGVESLIGHFPLLWWHELKYPGVPEFVAKFRKLAGGDPPENQGWGDYTAIKALGEVVR
                     KVNSTDSKAIVKGMEGFQFDSYKGRTLYFRELDHQLIMPMYTVRTKKKDEMKDKWEIF
                     EVIAEVPAKGQSAETIAIPKAESLCTKMEPLD"
     gene            4244..5119
                     /locus_tag="AC16G0_11300"
     CDS             4244..5119
                     /locus_tag="AC16G0_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5100233.1"
                     /translation="MSWLALMEYLLNGFVMGTLYVLMAIGLSLIFGMIGIINFCHGIL
                     FTLGAYATVALGNSLGFGPSLVVSPLLVGVLGMLIETVFLRRLYREDPASGLLLTFGL
                     AMVAEQCVRIVWGLNPLPYNIPSWLGGPLTLGFFLYPKYRYFILACAVAIIAALWWFL
                     EKTPYGMIIRAGSRDGEMVRILGISLRPIFTFVFGLGSALAAIAGVLMAPVAGVQPAM
                     GTDVGTAAFVVVTIGGLGSFWGAVIAGILVGEVVSLSILLYPPIAQASMYILMTVVLL
                     TRPRGLLGERCEKFE"
     gene            5116..>5860
                     /locus_tag="AC16G0_11305"
     CDS             5116..>5860
                     /locus_tag="AC16G0_11305"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5100234.1"
                     /translation="MSAPAKSAWSVTTLLGHPLTRLFAIFLVLPFVVVRLGTTYSIAT
                     EILIFSLMALAFNILLGYGGLLSFGHGAFFGIGAYGVAMTYTHLVKGMAVPMLVGTTA
                     ATGMGAVIGFLLMRKRGVYFALLTLAFTQMFFYIVYRATGVTGGENGIGGIERSVMAG
                     LDFTHQLTYYYLVAAVVSLAIGLVWRLVHSPFGRVLLATRENERRANCVGYDPKRYKL
                     IAFTISCGLAGLAGTLHAFLLNFVYPETLH"
BASE COUNT          962 a         1886 c         1959 g         1053 t
ORIGIN      
        1 cgctggagcg ccaagacgct gaagggcgag ggggaatact cggagcactg cctggccttt
       61 cccttcgtgg tctacctgct gggatttcgc caggaccagt tcgaagagat gcgcgtctac
      121 ttccgccccg ccccgctcct ccacgaggcc gatccccttt acttctccaa cctgtggaac
      181 gtgcaggcgg cggagagtcc tctggcgcgc tgccgggcgt gcctgcgcgg gcggccggag
      241 atccaggagc gtccgatggc ggagcaggcg gccagcctcg tcgagttctt ctggaccgcg
      301 gggttcaatc gagacatcga ggagaactgc ttcgaccgcg cccggggacg ggacccgcgc
      361 atcggcagtc tggaggcctg ggaggccgcg acgctggccg atccgctgtt ccccctctcc
      421 ctctcgtggg agccggtggg gctcagcctg ggggaggccc tggaccactg gcgccgccac
      481 ggggaccatg cccgccgagt cgagaccgcc gcggatgtgg cggacgtgat gtatcgcctg
      541 catgaggccc ccggatcgga gtccggggca ggcccgggaa catggtccgg gacaggccgc
      601 tgatgtttcc cgtgtatctc aaggacgagg ggttcgagtc gccggacgat cccatctact
      661 atcttgtgac ggcggacggc ctgttccagg tgaaggaaaa cccggtcttc cgctcctgcg
      721 tgggcgtgcg gggaatccgg gggcttcagg ggcaaaagga cgaggtgcgc tggaagctcc
      781 cgccgatccc gggcgccctg ctgcagcgcg tggtgggact cttccgcgaa gtgctccggc
      841 gctaccggag cgaggccatt gtcctcttga tctacgacct cgcgacgcgg gaatacgggg
      901 tcgtcgtccc ggagcagcag gtgacgggcg gtcatctgag ctacgccccg ggcgagacgc
      961 cgcccggacg catccgcgtc gggaccatcc acagccatgc cgaggcggat gcgtttcact
     1021 cccccctgga cgactgggac gaggagcacg atgacggtct gcacgtcacc gtggggaacc
     1081 tcgacgccag cgtgagcctt tcctgctccc tggtggtgga cggccgccgc ttcctcctgc
     1141 ggtcgcggga tgtcttcctg cccgcgccgc tggatacgcc cgagcaggtc tggttcgagg
     1201 gcctcaccca catccatacc gagccgccgg cgcgcgactc gttggacggc agaccgtcgg
     1261 cgatttgaga accggcatca gctcttcacc gtcggccccc cagcctgatc ctcgccctcg
     1321 agggggaggg gagggggggt gacgcacggc tgacagcgaa ccgctgatgg ctgacggcta
     1381 tcctatgcgg cacatcaagg tcatcggggt cggcggcatc gggtgcgcgc tcctgccaca
     1441 cctcgcccgg tacctccagg cgaagggcga agcggcgcgt ctgaccctcg tggacggcga
     1501 tgccttcgag gcacagaacg ccgatcgcca gcccttcagt cacctgggca acaaagccaa
     1561 agtgaaggcc gtggagctgg cccagcagtt cgaggggctg tccttccggg cggtgacgga
     1621 gtatgtgacc cgcgagaatg tggagacgac catcgggtcc ggggacgtgg tgttcctcgg
     1681 ggtggacaac cacgccacgc gacgggtggt gagcgaccgg tgccagcgtc tggcggaggt
     1741 caccctcatc tccggcggga acgactacac cgacgggaat gtgcaagtgt acctccggcg
     1801 cgaggatcgg gacgtcacgc ttcccctgac ccgcttccac cccgagatcg ccgacccacg
     1861 ggatcgctcc ccgcacgagc tgtcctgcga ggagctgtcc cgcgaagccg ccccgcaact
     1921 gctcttcgca aatctggcgg tggcctcggc catgctcaac gccttctatg cctgcgacgt
     1981 ggggaagctt cagtacggcg aggtctatct ggacgtcctg gagggcagag ccaacccgat
     2041 cgcgcgcgag ccagctcccg acgcgcagga cctcgggacg tgagtgccgg cgtctttcag
     2101 actccccggc gcaggagagt gggccggtgc gcgggggcgc ccatgggaag atacccgcct
     2161 tgccgattgt ccggttttcc gatcctcttt ccatcacact cttgcccgcc gcaacgttta
     2221 cgggggatat tcgccccatg catacgccga aggccaagat tcctcgtcag gtatccctcg
     2281 tgtgcctgcg cggtctgccc gacgagcctc ccccctcgca cgcgtcccgt gatcgggaag
     2341 agcagaccca cccggggtcc tcgtcctgct ggacggctta ctatggggag ggaagggagg
     2401 tggaccggca gcgggctgct ggcgcggcga cagaccgtgc gccaaggccg cggcggggag
     2461 ggggaagccg aaggcagagg ggagattcat gacgacagac aaccggatcg ccgatggctg
     2521 ctccgcccgt cgcaggctct tcacgagcga gggcagggtg ggcgatccgg tattccgttc
     2581 ccctgccctt ctgccccttg cactccatca ccgttcaggc ctatagtccc cgccgtgcat
     2641 cgtcagcaac ctccctggaa agcgcccttg accatcccat tcgagcggac gactgccacg
     2701 ctctgtccct ggctggatct ttacgctgcc aggttgaaga ggtaggccga gccggactca
     2761 gcattcttta tcgagcgtgc ttttcttggg aaaggaggtg gggcgtggcc gcgggctggt
     2821 agtctcagcc caaggaatat ccttagccga ggaccttcag gattattggg agaaggcaag
     2881 gagggaaacc atgaagcgac gcagcaacac ggcacgaggc ggaatcaccc gccgcggatt
     2941 cctgaagacc gtgggcgtgg tgggcggtgc ggcggccggc ctggggttcc ccgcggtcct
     3001 gcgggcgcag caagagccga tcaagatcgg ccacatttcc ccgcagacgg gattcctggc
     3061 ggccatcggc aagtattcgg atgcgggcgc gcagctggcg gtggatgaga tcaacgagaa
     3121 gggcgggctc ctcggccgca aggtccagtt gatcaatgag gacgaggcga acccgggcgt
     3181 cgccgcgcag aaggcccgga agctcatcga gaaggacaag gtcatcgccg tcaccggcat
     3241 cgtcagcagc gcctcggcct tggcggtcgg ggacgaggcc cagcgggcga aggtcctgtt
     3301 catcaacgcc ggggccaact cggacgagat ccggggggcc cgctgtcacc gctacgtctt
     3361 cagcacggag ggcagcaaca cccagtacgt gagcgccatc gcgagctggc tcttgcagca
     3421 gccgaagctg aagcgctggt acttcctcac gtccgactac gccttcggtc acgatctgct
     3481 caaggtggcg cagaagctcc tgcagaaggc cggccgggag caggtcggaa gcgaactggt
     3541 tccgaccggc agcgccgact tcagctcata cctgctgaag gtcaaggcgg cgaagccgga
     3601 cctggtgttt cagaatctcg caggctccga ccagaccaac ttcctgaagc agttcaaaga
     3661 gttcggactg gacatggaag tcgccggcgg cctggtggat acggccgtgg tgtggccggt
     3721 gggggtggag tccctgatag gccacttccc cctgctgtgg tggcacgagc tgaagtatcc
     3781 cggtgtgccc gagttcgtag ccaagttcag gaagctcgcc ggaggggacc cgccggagaa
     3841 ccagggctgg ggagattaca cggccatcaa ggccctcggc gaggtggtca ggaaggtcaa
     3901 cagcaccgac agcaaggcca tcgtgaaggg catggagggt ttccagttcg actcgtacaa
     3961 gggccggacg ctgtatttcc gggagttgga tcatcagctc atcatgccca tgtacaccgt
     4021 ccggaccaag aagaaggacg agatgaagga caagtgggag atcttcgagg tcatcgccga
     4081 ggtgccggcc aagggacaat ccgcagaaac catcgccatc ccgaaggcgg agagcctttg
     4141 caccaagatg gagccgctgg actgaggcct acccggggcc gggccgcatc ccgcggcccg
     4201 gccccttccg tcctgcatcc atgcagaggt gaggagcacg accatgagtt ggctggcgct
     4261 gatggaatac ctgttgaacg gcttcgtgat gggcaccttg tacgtgctca tggcgattgg
     4321 cctttccctg atcttcggga tgatcggcat catcaacttc tgtcacggga ttctgtttac
     4381 cctcggcgcc tatgccaccg tggccctcgg caatagcctc gggtttgggc cctcgctggt
     4441 ggtctcgcca ctcctggtcg gcgtcctggg gatgctcatc gagacggtct tcctgcggcg
     4501 actgtaccga gaggatcctg cctcgggact cctgctcacc ttcggcctgg ccatggtcgc
     4561 cgagcagtgc gtgcggatcg tgtggggcct caaccctctt ccctataaca ttccgagctg
     4621 gctgggtggt cctctgacgc tggggttctt cctctacccc aagtaccgct acttcatcct
     4681 ggcctgcgcc gtcgccatca tcgcggccct ctggtggttc ctggagaaga ccccctacgg
     4741 catgatcatc cgggcgggga gccgggacgg ggagatggtc cgcatcctgg ggatcagcct
     4801 ccgcccgatc ttcaccttcg tcttcggtct gggatcggcc ttggccgcca tcgccggtgt
     4861 gctcatggcg ccggtagccg gcgtgcagcc agccatgggg acggatgtgg gcacggcggc
     4921 cttcgtggtg gtgaccatcg gggggttggg gagcttctgg ggtgcggtca tcgccggcat
     4981 cctggtgggc gaggtggtca gcctgagcat cctcctgtat ccgcccatcg cgcaagcctc
     5041 catgtatatc ctgatgacgg tggtccttct cacccgcccc cggggtctcc tgggcgagcg
     5101 atgtgagaaa ttcgaatgag cgcgccggcg aagtccgcct ggtccgtgac gactctgctg
     5161 ggccatcccc tgacccgtct cttcgcgatc ttcctcgtgc tgcccttcgt cgtggtgcgg
     5221 ctggggacga catactccat cgccacggag atcctgatct tctcgttgat ggcactggcg
     5281 ttcaacatcc tgctgggtta cggcggcctt ctgtccttcg gccacggggc cttcttcggc
     5341 atcggggcct acggagtcgc catgacttac acccatctgg tgaagggcat ggccgtgccc
     5401 atgctcgtcg gcacgaccgc ggcgaccgga atgggtgcgg tcattggttt tctcctgatg
     5461 cggaaacgcg gggtctactt tgcgctcctg accctcgcct ttacccagat gtttttctac
     5521 atcgtatacc gcgccaccgg ggtgaccggg ggtgagaacg ggatcggggg gatcgagcgc
     5581 tccgtcatgg ccggcctgga cttcacccat caactgacct actactacct cgtggccgcc
     5641 gtggtcagtc tggccatcgg cctcgtatgg cggttggtcc actccccctt cggacgggtc
     5701 ctgctggcca cccgggagaa cgagcggcgc gcgaactgcg tcggctacga tcccaagcgg
     5761 tacaagctga tcgccttcac catctcctgc ggcctcgcgg gactggccgg gacactccac
     5821 gccttcctcc tgaacttcgt ctaccccgaa acgctgcacg
//
LOCUS       DBNGZC010000648         1710 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1032210, whole genome shotgun sequence.
ACCESSION   DBNGZC010000648 DBNGZC010000000
VERSION     DBNGZC010000648.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1032210"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="AC16G0_11310"
     CDS             <1..686
                     /locus_tag="AC16G0_11310"
                     /inference="COORDINATES: protein motif:HMM:NF023401.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 4 glycosyl hydrolase"
                     /protein_id="HIR5100235.1"
                     /translation="RGLTLDGEELWPRVFPAYLEGLRREADPEWDVRTLESLGMIPNY
                     YLQYFYYTDKKLRSQAEWPPSRAEEVMAIEAILLRDYADPGLTEPPADLMKRGGAYYS
                     TLATELIDSHFNDLGQVHVVNVRHNGAVKGWPEDWVVEMPARVDRQGIHPLPADPLPA
                     ACYGLVAQIKMYELLTVEAGVHGDRKAAYQALLAHPLGPRADKVQDVLDDMLETNRGW
                     LPQFSKPEA"
     gene            686..1681
                     /locus_tag="AC16G0_11315"
     CDS             686..1681
                     /locus_tag="AC16G0_11315"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013986.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HIR5100236.1"
                     /translation="MRYLLGIDVGGSKTHALIADESGCAVGFGLAGTGNYQGVGYDGY
                     RSSLEASVGQALRQAGIQPDQISGAGFGIGGYDWPSQLPAHQNVVASLGLTCPVEIVN
                     DAVIGLVAGTSEGWGVAIVGGTGCNCRGRDRQGREGRVTGEGERFGEWGGGYELVARA
                     IQAVSHEWSRRGPHTELTNTFLQMTGAKDLDDLIEGLDLGRYHPNARWAEVVFRVGYA
                     GDPIAREVVTWSGHELGELACAVIRQLALQDEAVQVVQIGGLFNGGPLYVDAVHETVL
                     RLAPRAQFVRLTVPPVIGGVLLGAAAAGISPSSIRGQLIQSTEALLHTAGPAQAS"
BASE COUNT          301 a          523 c          592 g          294 t
ORIGIN      
        1 atcgcggtct caccctggat ggcgaggagc tttggccgcg cgtcttcccg gcatatttgg
       61 agggactgcg ccgtgaagca gaccccgaat gggatgtgcg cacgctcgaa tcgctgggca
      121 tgatcccgaa ctactatctg caatacttct attacaccga caagaagctc aggtcccagg
      181 ctgagtggcc gccctccagg gccgaggagg tgatggcgat cgaggccatc ctgctgcgcg
      241 actacgcaga ccccggcctc acggaaccgc cggcggactt gatgaagcgg ggaggcgcct
      301 actactccac gctggcaacg gagcttatcg attcccattt caacgacctt gggcaggtgc
      361 acgtcgtcaa cgtccgccac aacggggccg ttaaaggctg gcctgaggat tgggtggtcg
      421 agatgcccgc ccgcgtggac cgtcagggca tccatccgct tccggcggac ccgctgcccg
      481 ccgcgtgcta tggcctggtg gcccagatca agatgtacga attgctcacg gtggaagccg
      541 gggtgcacgg cgaccgcaag gcggcctacc aggcactgct ggcccatccc ctcggcccca
      601 gggccgacaa ggtgcaggac gtgctggatg acatgctcga gaccaaccgg ggttggttgc
      661 cgcagttctc caaaccggag gcgtgatgcg ctatctgctt ggcatcgatg taggtggctc
      721 caagactcat gccctcatcg cagatgagag cggttgtgcg gtgggcttcg gcctggcggg
      781 caccggcaac taccaggggg tgggctatga tgggtatcgc agcagtctgg aggcgtcggt
      841 ggggcaggcc ttgcgccagg caggcattca gcccgatcag atctccggcg ctgggttcgg
      901 catcggcggc tacgattggc cctcgcagct tccggctcac cagaatgtgg tcgccagcct
      961 cggattgacc tgccccgtcg agatcgtgaa tgacgcggtg atcggcctgg tggccggcac
     1021 cagtgagggc tggggcgtcg cgatcgtcgg cggtacaggc tgcaactgcc gggggcgcga
     1081 ccgccagggt cgggagggac gcgtcaccgg cgaaggggag cgctttggcg aatggggcgg
     1141 cgggtatgaa ctggtggcga gggcgatcca ggcggtcagc cacgagtgga gccgccgcgg
     1201 tcctcacacg gagctcacca acaccttcct gcagatgaca ggcgcgaagg acctcgacga
     1261 tctcatcgag ggcctcgacc ttggaagata ccacccgaat gccaggtggg ccgaggtcgt
     1321 attccgcgtc ggatatgccg gtgacccgat tgccagggag gttgtcacat ggtcgggaca
     1381 cgagctgggc gagctggcct gtgcggtgat ccgtcagctt gccctccagg atgaggccgt
     1441 tcaggtcgtc cagatcggcg gtctattcaa tggagggccg ctctatgtgg atgccgtgca
     1501 cgagacagtc ctgcggctcg cgccccgcgc ccaattcgtg agattgaccg ttccaccggt
     1561 catcggcgga gtgctgctcg gagccgcggc agctggcatc agcccgagct cgatccgcgg
     1621 ccagctcatc caatcgacag aggcgttgct gcatacggcg ggaccagcgc aggcaagcta
     1681 gcggaaaccc gaagaaacct ccggggtctc
//
LOCUS       DBNGZC010000649         2731 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1426520, whole genome shotgun sequence.
ACCESSION   DBNGZC010000649 DBNGZC010000000
VERSION     DBNGZC010000649.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1426520"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="AC16G0_11320"
     CDS             complement(<1..235)
                     /locus_tag="AC16G0_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5100237.1"
                     /translation="MRRARTGEPSELDLLAIACLLPSLLYIFVLFIYPFLYGIYLSAH
                     PLKVPGFSLANYFAFFSDQYQYKTVSITFRLAVP"
     gene            complement(232..1317)
                     /locus_tag="AC16G0_11325"
     CDS             complement(232..1317)
                     /locus_tag="AC16G0_11325"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010099749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5100238.1"
                     /translation="MKETIESLRFRITKRFGTKTVLRDFALEVRGKQFITFLGPSGCG
                     KSTALNIIAGLIPATDGEVWIDGERIDHLPPEKRGFGMVFQNYALFPHMTVFGNVAFG
                     LQLQGRPAAEITERVQQMLDLVQLPGFEGRYPGQLSGGQQQRIAIARALVLHPRLMLL
                     DEPLSNLDAKLRIEMRAEIKRLHTSLGLTSIYVTHDQTEALSLSDWIVVMKDGVLIQA
                     GPPQEIHDQPVNIFVADFMGFRNFFPTQVVALGPDRHVEAAGGGMKIRGKTRHTFAPG
                     ASVVAAIRPDDVVVGGDAGSPNAFRATVDIVEYLGREDEAIVTLEAGGRLWVRTAARV
                     TPGENVTVVLPPDRVIFLSAESSGGTA"
     gene            complement(1395..2573)
                     /locus_tag="AC16G0_11330"
     CDS             complement(1395..2573)
                     /locus_tag="AC16G0_11330"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5100239.1"
                     /translation="MQRRGRFLGLLVLAALVTLGLTLGGVLPPASAGQPITISIIDNG
                     GDLASTGVIIENYKKANPDKVKEIKIQRGPAPETPAKIKAQQDAGRADINLVLTGQDA
                     GSVLVANKQLIELFPKYDKMFPKSELTDAAKVLQDSGGGALIPVVGNAGGPVFIYNPK
                     KVQKPPKTADELMAWVKANPNRFLYARPANSGPGRSIMAGLPFILNDKNPADPEKGWD
                     KAWAFMKELGKSMEYYPTGTLFTLREFAQEQRWMIAGIMEWDMKPRAEGVIPPDAKIT
                     VLDNTTFVIDGHYWGIPKGVPQNEVDVILDLMKFMRRVDQQVLTWKAFIGPSIKAATF
                     DKAPADIRDLVKEHWRPEYDQIGPGKKYKMAPQLDVKELTYAMDRWDREVGAQQIKKQ
                     "
BASE COUNT          462 a          863 c          880 g          526 t
ORIGIN      
        1 tgggaacggc aaggcggaag gtgatactca cggtcttgta ctgatactgg tcgctgaaga
       61 aagcgaagta gttcgccagg ctgaagccgg gcaccttgag cgggtgggca ctcaggtaga
      121 tgccgtagag gaacgggtag atgaacagca cgaagatgta gagcagcgag gggagcaggc
      181 aggcgatggc cagcaggtcc aactcgctcg gttcgccggt gcgtgcgcgc ctcatgccgt
      241 ccctccggag ctttccgccg aaaggaagat gacccggtcc ggcgggagga ccacggtcac
      301 gttctctccc ggggtcaccc gcgcggccgt ccggacccag aggcgcccac cggcttccag
      361 cgtcacgatc gcctcatcct cgcgccccag gtattcgacg atgtccaccg tggcccgaaa
      421 cgcattcggg ctcccggcgt ccccgccgac cacgacatca tccggacgga tggcggccac
      481 gacggatgcg ccgggtgcga aggtgtgtcg ggtcttgccc cggatcttca tgccgccgcc
      541 cgcggcctcc acgtgccgat ccggcccgag ggccacgacc tgcgtcggga agaagttccg
      601 gaaccccatg aagtccgcca cgaagatgtt gacgggctgg tcgtgtatct cctgcggcgg
      661 acccgcctgg atcaggacgc cgtccttcat caccacgatc caatcggaaa gcgagagggc
      721 ttccgtctga tcgtgggtca cgtagatgga ggtgagaccc aggctggtgt gtagccgctt
      781 gatctcggcg cgcatctcga tccgaagctt ggcgtcgagg ttcgacagcg gctcgtccag
      841 gagcatgagg cgtgggtgca ggaccagggc cctggcgatg gcgatccgct gctgctggcc
      901 gccggagagc tgtcccggat agcgcccttc gaagccgggc aactggacca ggtcgagcat
      961 ttgctggacc cgctcggtga tctctgccgc tggccggccc tgaagctgca gaccgaaggc
     1021 gacgttgccg aaaacggtca tgtgcgggaa gagagcgtag ttctggaaga ccatgccgaa
     1081 gccccgcttc tcgggaggca ggtggtcgat gcgctccccg tcgatccaga cctcgccatc
     1141 ggtggcgggg atcagcccgg cgatgatgtt cagggccgtg gacttgccgc agccgctggg
     1201 ccccagaaac gtgatgaact gcttaccgcg gacctccagg gcaaagtctc gcagcacggt
     1261 cttggtccca aatcgcttgg tgatccggaa tcgcagtgat tcaatcgtct ctttcatggt
     1321 cccccgccgg tgggcaagga aagggagggc cctcggccgg gccctcctct tggtgagtat
     1381 ccgcgagccc cctgctactg cttcttgatc tgctgggcgc cgacttcccg gtcccagcga
     1441 tccatcgcgt aggtcaactc cttgacgtcg agctgcgggg ccatcttgta cttcttgccg
     1501 ggaccgatct ggtcatactc cggccgccag tgctccttga cgaggtctcg aatgtccgcc
     1561 ggcgccttgt cgaaggtcgc cgccttgatg gaggggccga tgaaggcctt ccaggtcagg
     1621 acttgctggt cgaccctgcg catgaacttc atgaggtcca ggatgacgtc cacctcgttc
     1681 tggggcacgc ccttggggat gccccagtag tggccatcta tgacgaaggt ggtgttgtcc
     1741 aggacggtga tcttggcatc tggcgggatc acgccctcgg cccggggctt catgtcccac
     1801 tccatgatgc cggcgatcat ccagcgctgc tcctgagcga actcccggag ggtgaacagg
     1861 gtgcccgtgg gataatactc catggacttc cccagctcct tcatgaaggc ccaggccttg
     1921 tcccagccct tctccggatc cgcgggattc ttgtcgttca ggatgaatgg caacccggcc
     1981 atgatggacc ggccggggcc gctgttcgcc gggcgggcat agaggaaccg gttggggttc
     2041 gccttgaccc aggccatcag ctcgtcggcg gtctttgggg gcttctgcac cttcttcgga
     2101 ttgtagatga acaccggccc gccggcattc cccacgaccg gtataagcgc gcccccgccc
     2161 gaatcctgca gcaccttcgc cgcgtcggtc agctcactct tggggaacat cttgtcgtac
     2221 ttgggaaaaa gttcgatgag ctgcttgttt gccacaagga cggagcctgc atcctggccg
     2281 gtgaggacca ggttgatgtc cgcccgcccg gcgtcctgct gcgccttgat cttggcgggt
     2341 gtctccgggg ccggtccgcg ctggatcttg atctctttca ccttgtcggg attggccttc
     2401 ttgtagttct cgatgatcac gccggtggag gcgaggtcgc cgccgttgtc gatgatcgag
     2461 atggtgatgg gctgcccggc tgaggccgga ggcaggaccc cgccgagagt gagccccaac
     2521 gttacgaggg ccgccaggac caagagcccg aggaaccttc cgcgacgttg catcatgatc
     2581 ctccttttgc ggcctgcctc ccaccgaccg acctgacatg agtgacctcc accttccacc
     2641 tccccagtca cacttctacg aagtctacag agaggctatt ggcagagtca aggtcaatcg
     2701 ggtcttcagc gcctagacca gacaagcgat g
//
LOCUS       DBNGZC010000650         3779 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_937827, whole genome shotgun sequence.
ACCESSION   DBNGZC010000650 DBNGZC010000000
VERSION     DBNGZC010000650.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_937827"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..3219)
                     /locus_tag="AC16G0_11335"
     CDS             complement(100..3219)
                     /locus_tag="AC16G0_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5100240.1"
                     /translation="MTPELFIRRPVMTTLVMLGILFFGLLGFHALPVSDLPNVDFPTI
                     QVSASLPGASPDTMASAVATPLEKQFSTIAGVDSMTSTSALGLSNITIQFSLDRSIDG
                     AAQDVQAAIAKAAPQLPPGMPTPPTYQKVNPADQPVLYLALSSPTLPLYTVDEYAETL
                     LAQRISTINGVAQVQVFGSQKYAVRVQLDPNALASRGIGIDEVQKALADGNVNLPTGT
                     LYGPYQAFNVQATGQLTDAAAYRPLIVAYRNGSPIRLEQLGQVIDSVQTDKVASWFNG
                     ERAVVLAIQRQPGTNTVEVVDSIRKLLPTFRAQIPASVNLNVLYDRSISIRDSVRDVE
                     FTLLLAIMLVILVIFLFLRNLSATIIPSLALPMSIVGTFAAMYLLGYTVDNLSLMALT
                     LSVGFVVDDAIVMLENIVRHMEEGKTRMEAAREGAREIGFTIISMTLSLAAVFIPVFF
                     MGGVIGRLLHEFAATIGAAVLVSGFVSLTLTPMACSRFLRPPTESHGRLYAASERVFD
                     GMLGLYERTLKRVLQHPRLIMGVLFLTVVATGYLVLVIPKGFLPDEDTGQIFVFTEGA
                     QDISFDAMKQNQRRAAEIVQKNPYVDNFMSSMGATSFSPSPNTGRMFIRLKPHSERPS
                     AGQIVQDLRRQFAALPDTRVYPQILPTIRIGGQLTKALYQYTLQDADLKELYRWAPMV
                     FDKMRGLPGLQDVNTDLQITNPQVLVKIDRDKASTLGVTAQQIENALNYAYGSPQVST
                     IYTPSNEYWVMMELLPRYQAHPADLSLLYVRSAGGQLVPLNAVASLIRGVGPLTVNHL
                     GQLPAVTISFNLKPGVALGDAVGAIQKMQRDLQLPATLSTSFQGTAQAFQASLQGLGL
                     LVLAAIVVIYLVLGILYESFIHPLTILSGLPSAGVGALLTLLLFRMELNLYGFVGIIM
                     LIGIVKKNAIMMIDFAQQAEARGKSPADAIFEGCLLRFRPIMMTTMSALMGTLPIAIG
                     FGAGADARRPLGLTVVGGLILSQLLTLYITPVTYLYLEKIRQWLFRKRAAGPAMKEDP
                     VFAVNSPDR"
     gene            complement(3216..>3779)
                     /locus_tag="AC16G0_11340"
     CDS             complement(3216..>3779)
                     /locus_tag="AC16G0_11340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5100241.1"
                     /translation="KARDSNSPLVVINQVHPIYVSFSVPEQSLADIRTYRAAGTLQVN
                     ALIPGQEEKPVGGELSFVNNTVDPTTGTIQLKASFPNTDNRLWPGQFLNVLLTLTTEP
                     NAVVVPSQAIQTGQQGPYVFVVKPDLTAEMRSIAPGLVLDGDTIVQKGLSPGERVVTQ
                     GQIRLVPGIRVDIKSTAPSGAPQEKAG"
BASE COUNT          721 a         1090 c         1290 g          678 t
ORIGIN      
        1 agtgcccgat cagcggcggc gaataccttg gcgaatggag atctggccgg cctggccttt
       61 cagagagagg tcaatccgcg gcggcactgc ccgagcgggc tacctgtccg gggaattgac
      121 ggcgaagacg ggatcttctt tcatcgcggg gccggcggcg cgcttccgga ataaccactg
      181 gcggattttc tctagataca aatacgtaac gggggtgatg tagagcgtca ggagctgcga
      241 gaggatcagg cctccgacga cggtgagccc cagggggcgg cgcgcatctg cacctgcccc
      301 gaacccgatg gcgattggca gcgtgcccat gagggccgac atcgtcgtca tcatgatggg
      361 gcggaaccgg aggagacacc cctcgaagat tgcgtccgca ggactcttgc ccctcgcctc
      421 cgcctgctgt gcgaagtcga tcatcatgat ggcattcttc ttgacgatcc cgatcagcat
      481 gatgatgccg acgaagccgt acaggttcaa ctccatgcgg aacagcagga gcgtgaggag
      541 cgccccgacg ccggccgagg ggaggcccga caggatcgtg aggggatgga tgaagctttc
      601 gtacaggatc cccaggacca ggtagatcac cacgatggcc gccagcacca gtagccccaa
      661 cccctggagc gatgcctgaa acgcctgggc cgtcccctgg aagctggtgc tcagggtggc
      721 aggtagctgc aggtcccgct gcatcttctg gatcgctccc acggcatcgc ccagcgccac
      781 cccaggcttg aggttgaagg agatggtcac cgcgggaagc tgtcccaggt ggttgaccgt
      841 gagggggccg acgccccgaa taaggctggc cacggcgttc agggggacga gctgcccgcc
      901 ggcggagcga acgtacagca gcgacaggtc ggccggatgc gcctggtagc gcggaagcag
      961 ttccatcatg acccagtact cgttcgaggg ggtgtagatc gtggagacct ggggtgagcc
     1021 gtaggcgtag ttcagggcgt tctcgatctg ctgggcggtg acgcccaggg tcgaggcctt
     1081 gtcccgatcg atcttcacga gcacctgggg attggtgatt tgaagatcgg tgttgacatc
     1141 ttgaaggccc ggtagccccc gcatcttgtc aaagaccatg ggcgcccagc ggtagagttc
     1201 cttcaggtcg gcgtcctgca gggtgtactg atagagggct ttggtgagct gtccgccgat
     1261 gcggatcgtc ggcaggatct gcgggtagac tctggtgtcg gggagcgcgg cgaattgtcg
     1321 ccggaggtcc tggacgatct gccccgcgct cggtcgctcc gagtgcggct tgagacggat
     1381 gaacatgcgc ccggtgttgg gcgaagggct gaagctggtt gcccccatgg acgacatgaa
     1441 gttatccaca taggggttct tctgaacgat ctccgccgca cgccgctggt tttgcttcat
     1501 ggcatcgaaa gaaatatcct gggctccctc cgtgaacacg aagatctgtc cggtgtcctc
     1561 atcggggagg aaacccttcg gaatcaccag gaccaggtat cccgttgcca ccacggtgag
     1621 aaagaggaca cccatgatga ggcgcggatg ctgcagcacc cgtttgaggg tccgctcgta
     1681 cagtccgagc atgccgtcga agacccgttc ggaggccgcg tagaggcggc cgtgggattc
     1741 ggtcggcggc cggagaaagc ggctgcacgc cataggcgtg agagtcaggg agacgaagcc
     1801 ggacaccagc accgcggcgc cgatggtggc cgcgaactcg tggagcagcc gcccgatcac
     1861 accgcccatg aagaacaccg ggatgaaaac ggctgccaga gacagggtca tcgaaatgat
     1921 ggtgaaaccg atctcccgag cgccctcccg ggccgcttcc atgcgggtct tgccctcttc
     1981 catgtgccga acgatgttct ccagcatgac gatggcatcg tccacgacga atccgacgga
     2041 aagcgtgagg gccatgagcg agaggttatc aaccgtgtag cccagcagat acatagctgc
     2101 aaatgtgccc acgatggaca tcggcaaggc caagctggga atgatcgtcg ccgacagatt
     2161 ccgcagaaaa aggaagatca cgaggatgac cagcatgatg gcaaggagca aggtgaactc
     2221 gacgtcacgg accgaatcgc ggatggagat cgagcggtcg tacaggacgt tgaggttcac
     2281 cgaggcaggg atttgtgccc gaaaagtggg cagcagcttt cggatcgagt ccacaacctc
     2341 gaccgtattc gtgcctggct gcctctggat cgccaagacg acagcccgct cgccgttgaa
     2401 ccagctcgcc accttgtcgg tctgcacgct gtcgatcacc tggccaagct gctcgagccg
     2461 gatgggcgag ccgttgcggt aggcaacgat cagcggacgg taggctgcgg cgtcggtgag
     2521 ctgtccggta gcctgcacgt tgaacgcttg atagggaccg tagagcgtcc ccgtcggaag
     2581 gttcacgttc ccatcggcaa gggctttctg aacctcgtcg atgccgatgc ctcgcgaggc
     2641 cagcgcgttg gggtcgagct gcactcgcac ggcgtacttc tgcgagccga aaacctggac
     2701 ctgggccacg ccgttgatcg tggagatgcg ctgcgccagg agcgtttcgg catattcatc
     2761 gacggtgtac agcggcagcg tcggcgagct gagggcaagg taaagaaccg gctgatcggc
     2821 cgggttgacc ttttggtagg tggggggtgt gggcatgccc ggcggcaatt gcggggcggc
     2881 cttggcgatg gccgcctgga cgtcttgagc ggcgccatcg atgctccggt ccaggctgaa
     2941 ctggatcgta atgttggaaa ggccaagggc gctcgtcgac gtcatggaat ccacgccggc
     3001 gatggtgctg aactgcttct ccagaggcgt cgccaccgcg gaggccatcg tatccggact
     3061 ggcgccgggc aggcttgccg agacctggat ggtagggaag tccacgttgg gcaggtcgct
     3121 cacgggcagg gcgtggaagc ccaggaggcc aaagaacaaa atgcccagca tgaccagcgt
     3181 ggtcatgaca ggccgtcgga tgaagagttc cggagtcatc ctgctttctc ctgcggagcc
     3241 ccggagggcg cagtggactt gatgtccacc ctgatcccgg ggaccaggcg aatctggccc
     3301 tgggtgacga ctcgctctcc gggcgaaagg cccttctgca cgatggtgtc gccatccagt
     3361 accagtcctg gggcgatgga acgcatctcg gcggtgaggt cgggcttgac aacgaatacg
     3421 tacggcccct gctgacccgt ctggatcgcc tgagacggaa ccaccacggc gttcggctcc
     3481 gtggtgagcg tcagcaggac gttcaggaac tggccgggcc agagcctatt gtccgtattg
     3541 gggaagctgg ccttgagctg gatggtcccc gtcgtcgggt ctaccgtgtt gttcacgaag
     3601 ctcaactcgc cccccaccgg tttctcttcc tggccgggga tgagggcgtt gacctggagc
     3661 gtgccggctg cccggtacgt ccggatgtcc gccagcgact gctcgggcac ggagaagctc
     3721 acgtagatcg gatggacctg gttgatgacg acgagcggac tgttgctgtc tcgggcctt
//
LOCUS       DBNGZC010000651         1599 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1718619, whole genome shotgun sequence.
ACCESSION   DBNGZC010000651 DBNGZC010000000
VERSION     DBNGZC010000651.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1718619"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="AC16G0_11345"
     CDS             complement(<1..170)
                     /locus_tag="AC16G0_11345"
                     /inference="COORDINATES: protein motif:HMM:NF024736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR5100242.1"
                     /translation="MKPQRYPSDLTRTQWKRLQRLLPPAKPGGRPREVHLREVLNGIL
                     YIARGGCSWRMMP"
     gene            complement(242..1426)
                     /locus_tag="AC16G0_11350"
     CDS             complement(242..1426)
                     /locus_tag="AC16G0_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR5100243.1"
                     /translation="MRKSINPLTPTQVYCYAVQACQPHLKLGGPKKALGSMILTVVFA
                     AAARVSSLSDTCRRLQGVPDEHVVADALYATLPGYDVLRRRVQAALRGHLPKALRRKP
                     QIVALDLTLLPYYGADAKTNPLVVRAKAKKGTCSFFGYGTAYVVRTGWRYTLALTAVT
                     RDMTMADLARALLKQVRAAGIKVRFLVLDREFYSVAVIRYLQAARVAFLMPVVCHGRP
                     ADHPLGPSGSNVFKQCKRSGWSRYTLSDAAKRRATVRICIKCRNRRGERGQQGREAWI
                     YAYWGIGPKRFDWVKETYRRRFGIETSYRQMNQCGIRTTSKRFEVRFLYVAIALMLRN
                     LWVWLHHAVLSRPRRGGIELHLERLRLRTMLLWLFEVAAELYGLVNMALIERLLPKSV
                     TT"
     gene            1538..1599
                     /locus_tag="AC16G0_11355"
                     /pseudo
     CDS             1538..1599
                     /locus_tag="AC16G0_11355"
                     /inference="COORDINATES: protein motif:HMM:NF019228.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          294 a          504 c          523 g          278 t
ORIGIN      
        1 ggcatcatcc gccacgagca accgccccgc gcgatgtaga ggatgccgtt gagaacctcg
       61 cggaggtgga cctcgcgcgg ccgtcccccg ggcttggccg gggggagcag gcgctggagt
      121 cgtttccact gagtgcgggt caggtcggag ggatagcgtt ggggtttcat acgggaattc
      181 taggctcgac ctgacttttc gtacaccctt tcagtagttc ggtactacgg ctttcggaga
      241 cctacgttgt aacagatttc ggcaaaagtc tttcgatcaa tgccatgttc accaggccat
      301 acaactcggc ggccacctcg aagagccaca gcagcatcgt cctcaggcgc aatcgctcca
      361 ggtggagttc gatgccaccg cgacgcggcc gggagagcac cgcatgatgc agccacaccc
      421 acaggttccg cagcatcaac gcgatcgcca cgtacaagaa ccgcacctcg aaccgcttcg
      481 acgtcgtccg aatcccacac tggttcatct gacgatagct ggtctcgatc ccgaacctcc
      541 tccggtacgt ctccttgacc cagtcgaacc gcttcggacc aatcccccaa taggcataga
      601 tccaggcctc ccgaccctgc tggccgcgct cgccgcgacg gttccggcat ttgatgcaga
      661 tccgcaccgt ggccctgcgc ttcgccgcgt cggacagcgt gtaccggctc cagccgctcc
      721 gcttgcactg cttgaagacg ttcgagccgc tggggcccag cggatggtcg gccgggcggc
      781 cgtggcagac caccggcatc agaaacgcga cccgcgccgc ctggagataa cggatgaccg
      841 cgacgctgta aaactcgcgg tccaggacca ggaagcggac cttgatcccg gcagcgcgca
      901 cctgcttgag cagtgcgcgg gcgaggtcgg ccatggtcat gtcccgcgtc acggcggtca
      961 gcgcgagggt gtagcgccac cccgtgcgga ccacgtaggc ggtgccatag ccgaagaacg
     1021 agcaggtccc cttcttggcc ttggcacgga cgaccagagg gttggtctta gcgtcggcgc
     1081 cgtagtaggg gagcaacgtc aaatcgaggg cgacgatctg gggcttgcgg cgcagggcct
     1141 tgggtaggtg gccgcggagc gcggcctgaa cccgtcgccg caggacgtcg tacccgggaa
     1201 gcgtggcgta gagcgcgtcg gcgacgacgt gttcgtcggg gacgccctga agtcgccggc
     1261 aggtgtcgga cagagaactg acccgcgcgg cggcggcgaa tacgacggtc aggatcatcg
     1321 agccgagggc cttcttgggg ccgcccagtt tcaggtgagg ctggcaggcc tgaacggcgt
     1381 agcaatagac ttgggtgggt gtcagggggt tgatggactt gcgcatagga ccctccgtga
     1441 gtgttagggt ttgcacaagt cattctcaga ggacaggttg agtctgtcat cccctggcaa
     1501 ccctctccaa gccgatctac cgaactactg agtctatgac ctcagccgga accaggggtg
     1561 ggtcagcgtc ggcgtcgatc acgacaccgc cgagttcgc
//
LOCUS       DBNGZC010000652         1896 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1032443, whole genome shotgun sequence.
ACCESSION   DBNGZC010000652 DBNGZC010000000
VERSION     DBNGZC010000652.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1032443"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="AC16G0_11360"
     CDS             <1..337
                     /locus_tag="AC16G0_11360"
                     /inference="COORDINATES: protein motif:HMM:NF019228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAzo13-like element transposase-related
                     protein"
                     /protein_id="HIR5100244.1"
                     /translation="RDTVQTGLHELHHGMRCLENFAARGRQRSEQKDPRLAADIRALV
                     EPHTQADPELKSSRRYTNLSAAEVRHALIDKGYSNEELPAERTLRDILNRMNYRLKRI
                     QRLSGNFSL"
     gene            complement(314..1159)
                     /locus_tag="AC16G0_11365"
     CDS             complement(314..1159)
                     /locus_tag="AC16G0_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002653516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HIR5100245.1"
                     /translation="MRKPYPSDLTDQQWAIIQPLIPVHQVGRPRLVDMREVLNAIFYL
                     NRSGCQWDMLPHDLPAKSTVYDHFAQWKADGTWQAILDALRQQVRIAVGRDPNPSAGS
                     IDSQTVKATEVAEDRGYDGGQKITGRKRHIIVDTLGLLLVVLVTVASADDGTTAPQVL
                     GRLTTEHRTRLEKVWADGKYNNHHLNGWLVKTKAGYVIEVIGRPAGSVGYVKLPRRWV
                     VERTFAWLGRYRRNSRDYERFTESSEAMVKISSIHRMLRLLKTDSLKEPVRFKYRELQ
                     GKVPG"
     gene            complement(1264..1836)
                     /locus_tag="AC16G0_11370"
     CDS             complement(1264..1836)
                     /locus_tag="AC16G0_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100246.1"
                     /translation="MIEDTVRYPAETLTIAFPKQARELRVQVIRDILWYRGGKTEPIM
                     VVLVRDPLGQWRDEALVATDPTASAAFVIMGYCRRWSVELAFFDSKQSLGLHDPRVWS
                     ARSVERAHPMAWFIGSLTILWYCLEGHAGAHVVKDRPWYTTKVTPTFADMLGALRLQM
                     WEYAIYEECGDEPPSPECIQSLLHKLAAVA"
BASE COUNT          337 a          624 c          569 g          366 t
ORIGIN      
        1 acgcgatacg gtccagacgg gattgcacga gcttcatcac gggatgcgtt gcctggagaa
       61 cttcgccgcg cggggccgac agcgtagcga acagaaggac ccacgcttgg cggccgacat
      121 tcgcgccctc gtcgagcccc atacccaggc cgatccggaa ctgaagtcat cacgccggta
      181 caccaacctg tcggcggccg aggtgcgaca cgccctgatc gacaagggtt attcgaacga
      241 ggaattgccc gccgagcgca ccctgcgtga catcctcaat cggatgaact accgtctcaa
      301 gcggattcag aggctatccg ggaacttttc cctgtaattc tcggtatttg aaccgcaccg
      361 gctctttcaa tgaatcagtt ttcaagagcc tgagcatgcg atgaatcgaa ctgatcttga
      421 ccatcgcctc gctcgactcc gtgaaccgct cgtaatcccg gctgttccgc cggtaccggc
      481 ccagccaggc gaatgtccgc tcgaccaccc agcgcctcgg caacttcacg tacccgacac
      541 tcccggccgg acgaccgatg acctcgatca catacccggc cttggtcttc accagccatc
      601 cgttgaggtg gtgattgttg tacttcccgt cggcccacac cttctccaat cgggtccggt
      661 gctcggtggt cagtcgcccc aggacctggg gggcggtcgt accgtcgtcg gccgaggcca
      721 ccgtcaccag gaccaccagg agcaaaccca gggtgtccac gatgatatgc cgcttccgcc
      781 cggtgatctt ctggcccccg tcgtagcctc tgtcttcggc gacttcggtg gccttgacgg
      841 tctggctgtc gatgctcccg gcgctcgggt tggggtcacg accgacggcg atccggacct
      901 gctgccgcaa ggcgtcgagg atcgcctgcc aggtcccgtc ggccttccac tgggcgaagt
      961 ggtcgtacac ggtgctcttg gccgggaggt cgtggggaag catgtcccac tggcagccgg
     1021 agcggttcaa gtagaagatg gcgttgagca cttcccgcat gtcaacgaga cggggtcgcc
     1081 ccacctggtg cacggggatc agaggctgga tgatcgccca ctgctggtcg gtcaggtcgg
     1141 atgggtaggg cttacgcatc ggtgcatgct ccgtcgtgag tgaatcctgc gaacctcctg
     1201 ataaggtagg atgttacgat actcgggaat tcccggatag cctctaactt gcttttcgga
     1261 ctctcaggct accgccgcca gcttgtggag caggctttgg atacattccg gcgagggcgg
     1321 ttcatcacca cactcctcat aaatcgcata ctcccacatc tgcaatcgca gcgcaccgag
     1381 catgtcggcg aacgtcggcg tgaccttcgt ggtgtaccac gggcggtcct tgacgacatg
     1441 ggcacccgca tgaccctcca ggcagtacca caggatcgtc agcgacccga tgaaccacgc
     1501 catcgggtgc gcccgctcga cgcttcgcgc gctccacacc cgcgggtcgt gcaggccgag
     1561 ggattgtttg ctgtcgaaga aggccagttc cacactccat cgccggcagt atcccatgat
     1621 gacgaacgcc gccgaggccg tggggtcggt cgccaccaac gcctcgtccc gccactggcc
     1681 gagcgggtcg cgcaccagca ccaccattat cggctcggtc ttgccccccc gataccacag
     1741 gatatcgcgg atgacctgca cccgcagctc ccgcgcctgc ttcgggaagg cgatcgtcag
     1801 ggtctcggcg gggtagcgca ccgtgtcctc gatcatcacc tgctcagagg gaggggtttt
     1861 tcaggatggg tgtcgggggt tgtccctcgg atgtca
//
LOCUS       DBNGZC010000653         7096 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1524209, whole genome shotgun sequence.
ACCESSION   DBNGZC010000653 DBNGZC010000000
VERSION     DBNGZC010000653.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7096)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7096)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7096
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1524209"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="AC16G0_11375"
     CDS             <1..759
                     /locus_tag="AC16G0_11375"
                     /inference="COORDINATES: protein motif:HMM:NF013738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HIR5100247.1"
                     /translation="RYLTFGCTSAIASSEALCVLIEQGNYTPIGALQITNRDIVTYLG
                     GLPDQKIHCSVMGEEALEAAVFNWAQKRGVDLSVLGVDMHADEKEEGRLVCQCFGLTE
                     PYLKRKIRELGLKTIAEITGAVKAGGACMSCHHKPGGLQDLLTEVWGAQPLTLRVLPS
                     FPEPRPSAAADRPISSPYQFSKQVEKALDQYVRPMLAKDGGDLEIVDIKDTAVYCRLV
                     GACTGCGGAGMTMKLMVERTLKEMVDDRIRIITV"
     gene            770..1990
                     /locus_tag="AC16G0_11380"
     CDS             770..1990
                     /locus_tag="AC16G0_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002710757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR5100248.1"
                     /translation="MQSVYLDNNATTAVAPEVRKAMEVYFTSEYFNPSSAYDRASPAA
                     QAVKRARETVARFLGGVDPVEILFTSCATESLNAALFGAVRANPSRRHILTTAVEHPA
                     VYEVCKELERSGLEVTFLPVDRAGNLELRTFIRSLRPDTLIVAVMHANNETGVIFPIE
                     QLARITKETDPAILFLTDATQTAGKLPLDLSGALRHVDLLAFSGHKLHAPKGVGALFV
                     RRGTPIRPFLIGGHQEGGRRAGTENVPYIAGLARAVELAQEDHDGIEARIRRLRDRLE
                     AGLLAQIPCLEVNGQGALRLPNTSNISVHFIEGEGILYQLNEHGVFASSGSACTSGSL
                     EPSHVLRAMHVPFTAVHGSVRLSLGRYNTDEDVDKALEVFPHIVANLRRLSPYWDTTH
                     NRPRPEAEAMMREP"
     gene            2440..2937
                     /locus_tag="AC16G0_11385"
     CDS             2440..2937
                     /locus_tag="AC16G0_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5100249.1"
                     /translation="MGLSQALLPEFDQEMANTRKTLERVPEDKLGWKPHEKSGTMGWL
                     ANHVATLPRLAVFAIEQDSVDVATVPRPSPANSRQELLNTFDKHVAAARAAIAGASDE
                     RLLKPWSLLHSGRAIFTLPRTAALRSFFMNHSIHHRAQLGVYLRLNNVPVPAIYGPSA
                     DEPAF"
     gene            complement(2971..3639)
                     /gene="mtgA"
                     /locus_tag="AC16G0_11390"
     CDS             complement(2971..3639)
                     /gene="mtgA"
                     /locus_tag="AC16G0_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008212041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HIR5100250.1"
                     /translation="MWRAILALVLLTVTPVLSLRWIAPPTSAFMLEKRVGSILRREPQ
                     PVIRYRWIGWESISPQMRLAVVAAEDQKFPYHWGFDFESIADAVLEKDARGRLRGAST
                     ITQQVARNLFLWPGRSYVRKGLEAYFTLLLELLWPKRRILEVYLNIAEFGDGAYGVSA
                     ATQAFFGKRPSALQREEAALLAAVLPNPARLHVRNPSGYVRERASWIQEQMTHLGVAY
                     LRNL"
     gene            3889..5256
                     /locus_tag="AC16G0_11395"
     CDS             3889..5256
                     /locus_tag="AC16G0_11395"
                     /EC_number="5.4.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR5100251.1"
                     /translation="MAMLKHLESRAKEILARELKVYADKNSKSREAYERSCRVLPGGV
                     SRTLCYFPPFPCQTAYGEGCWMVDADGNRRLDLFNCATTLILGHRPPAVMKAVQEQLT
                     HGTAFQTNSGLETTLAEMLVERVASLEQMRFTNSGSEGTLMAVRAARAFTGRPKIAKM
                     EGGYHGTHDSVEISVRPPMDLAGSPEEPKAVAGSAGITPGTVGDVVLLPYNNLQAARA
                     ILAREASQIAAIIVEPILGSVGFVPAEREFLHGLRAEADKHGMLLILDEVQSFRLDRG
                     GAQQLFGVRPDLTCFGKIIGGGFPVGAFGGRRDVMALFNTATGKGTIPHAGTFNGNPI
                     TMTAGIATMEQLTEPVYSRLNSMGDRLREQLGQIGRRHEVPMQITGIGSMFKIHFSAS
                     PVRDYRSTLQASPLLHTALFLFSLNRGLFLSEGGRCCVSAAMGDAELTQYLGTVEEFL
                     AELTR"
     gene            5529..6917
                     /locus_tag="AC16G0_11400"
     CDS             5529..6917
                     /locus_tag="AC16G0_11400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5100252.1"
                     /translation="MTTASRSLAADLRRLLPPGQVLDTPEDRTLYAYDGTSSRALPDV
                     AVMARSAQDVSHILKYATAQSTPVVPRGAGTGLSGGSVPARGGIALVLASMRAIKEID
                     TGSMLAVVEPGVITGQFQKTVEEAGMFYPPDPASLNYCTLGGNVAENAGGPHGVKYGV
                     TKDYVLGLEVALPNGDVARLGGKQIKNVTGYNLVQLFVGSEGTLGVITEIILRLLPLP
                     PAKRTMLAIFDRLDDAATAVAGIMAGGVIPATLEIMDKKSINCVEDYLKLGLPRDAEA
                     ILLIEVDGMQAAVEAEAEVIGRICREKGVRSFQTAKDKKEEDSLWKARRSISPAISAA
                     RPSKIGEDISVPRSAIPAMVREIQRIAKEYDLPIVIFGHAGDGNLHPNILTDRRDHQE
                     MERVEQAIAAIFEASVKLGGTLSGEHGIGLSKQQFLKLALSPETVKLMADLKRAVDPQ
                     GILNPGKIFGKA"
     gene            6914..>7096
                     /locus_tag="AC16G0_11405"
     CDS             6914..>7096
                     /locus_tag="AC16G0_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5100253.1"
                     /translation="MMELTQLGNLFKCNKCGSCTSVCPLYQQTVYEGMAARGKLALLE
                     ATVEGKLDSPRAVRAKL"
BASE COUNT         1347 a         2213 c         2326 g         1210 t
ORIGIN      
        1 cggtatctta ccttcggctg cacctccgct atcgcctcct cggaggcgct ctgcgtcctg
       61 atcgagcagg ggaattacac cccgatcggc gccctccaga tcacgaaccg cgacattgtc
      121 acctacctgg gcggcctgcc agatcagaag atccattgct cggtgatggg cgaggaggcg
      181 ctggaggcgg cggtcttcaa ctgggcgcag aaacgggggg tggacctctc ggtcctcggc
      241 gtcgacatgc acgccgatga gaaagaggag ggccgcctcg tctgccagtg tttcggcctc
      301 actgagccct atctcaagcg caagatccgg gaactgggcc tgaagaccat tgccgagatc
      361 accggcgcag tcaaggcggg cggcgcgtgc atgtcgtgcc accacaagcc gggcggtctg
      421 caggacctgt tgaccgaggt ctggggggct cagcccctca cgttgagggt cctccccagc
      481 tttccggaac cgcgaccgtc tgccgccgcg gatagaccca tctcgtcgcc gtaccagttc
      541 agtaaacagg tggagaaagc cctggaccag tatgtccggc cgatgctggc gaaagacgga
      601 ggcgacctgg agatcgtcga catcaaggac accgccgtct actgccgcct ggtcggagcg
      661 tgcacggggt gcggtggggc cggcatgacc atgaaactca tggtcgagcg tacgctgaag
      721 gagatggtcg acgaccgcat ccgcattatc accgtgtgag ggccacccca tgcaatccgt
      781 ctacctggat aacaacgcca cgacagccgt cgccccagag gtccgcaagg cgatggaggt
      841 ctatttcacc tccgagtatt tcaatcccag ctcggcctac gaccgcgcct caccggcggc
      901 ccaggccgtc aagcgggcgc gtgagaccgt ggcgcgtttt ctgggggggg tcgacccggt
      961 ggagatccta tttacctcct gtgccacgga gagcctgaac gccgccctct tcggagcggt
     1021 ccgggccaat ccttcccgca ggcacatact cacgacggct gtcgagcacc cggcggtcta
     1081 cgaggtgtgc aaggagctcg agcggagcgg cctggaggtc acctttttgc ccgtggaccg
     1141 ggcggggaat ctcgaactcc gcacgttcat tcggtcgctc cgacccgaca ccttgatcgt
     1201 ggccgtcatg cacgccaaca acgagaccgg tgtgatcttc cccatcgagc agttggcgcg
     1261 catcaccaaa gaaaccgacc ccgccatcct ctttctcact gacgcgacac agacggcggg
     1321 aaaacttccc ctggacctct ccggggccct gcgacacgtg gatctgctcg cattctcggg
     1381 gcacaaactg catgccccca agggcgtggg cgccctcttt gtccgccgtg gtacgcccat
     1441 ccgccccttc ctgatcgggg gtcaccagga gggcgggcgc cgggccggca ctgaaaacgt
     1501 tccatatatc gcgggactgg cccgggcggt ggaactggct caagaagacc acgacgggat
     1561 cgaggcgcgc atcagacggc tgcgagatcg gctggaggcc gggctcctgg cgcagatccc
     1621 ctgcctggag gtcaacggcc agggagcctt gcgtctcccc aacacatcca acatctcggt
     1681 gcacttcatc gagggcgagg ggatactcta tcagctcaac gaacacggcg tcttcgcttc
     1741 atcggggtcg gcctgcacct cggggtcgct ggagccgtcc catgtccttc gagccatgca
     1801 cgtgcccttc accgccgtcc acggctcggt gcgcctatcc ctcggccgct acaacaccga
     1861 tgaggacgtg gataaagccc ttgaggtgtt cccacacatc gtggccaacc tacgccggct
     1921 ctccccgtac tgggacacaa cccacaaccg tcctcgcccc gaggctgagg cgatgatgcg
     1981 ggaaccgtaa cggtccggga cgttacggag cggtagggct caccgggtcc aaaattctgg
     2041 aagacaagtt attcgactgg gtggccaggg gattccgcat ccacgaacgg attcgagccc
     2101 tgacccctat ccgagaggtc cggctcaaga cgccgccttc agcgcgcacg gatccactgg
     2161 cggtcggcca tcggattggg tggcgacccc tcacccctat cggcaagcac cctggcgcaa
     2221 tgacgcagag aatcggggtt gcccttcctg ttctatttgc ttaccatacc aatacttgca
     2281 aacagatcac acacctcgat cctatcctcg ccggaggaca tttaggccgt ggctctcagc
     2341 ggtccatggg gaccgtattg agggaggtga actggaacta ttggcgcctt tgcccgtgcc
     2401 gtgatgtgcg gccctggaag gcgctggcat ggaggaacca tgggactgag tcaagcattg
     2461 ctgccggaat tcgaccagga gatggccaac acgcgaaaga cgctggagcg ggttcctgag
     2521 gataagctcg gctggaagcc tcacgagaag tccgggacga tgggatggct ggccaaccat
     2581 gtggccacgc ttccgcgctt ggccgttttt gccatcgagc aggactcggt tgacgtggcc
     2641 acggtgcctc gcccgtcacc cgcaaattcc cgccaggagt tgctgaatac attcgacaag
     2701 catgtggcgg ccgcgcgcgc cgccatcgcg ggggcgagcg acgagcgact cctgaagccc
     2761 tggagcctgc tccacagcgg acgggcgatc ttcacgctgc cgcggaccgc cgcgctgcgg
     2821 agctttttca tgaaccatag catccaccac cgcgcccagc tcggcgtgta cctgcgtttg
     2881 aacaacgtgc cggtgcccgc gatctacggg ccgtcggccg acgagccagc tttctgacct
     2941 ccgtggagcc gctgtctgga aggggacggc tcagagattc cgaaggtagg cgacgccgag
     3001 gtgagtcatc tgctcctgga tccagctcgc acgctcgcgt acgtatccgg agggattccg
     3061 gacgtggagc cgagcaggat tgggcaacac cgcagccagg agggcagcct cctctctctg
     3121 caaagcggat ggccgcttgc cgaagaaggc ttgggtggcc gcggagaccc cataggcgcc
     3181 gtcgccgaac tcggcgatgt tcaagtagac ctccaggata cgccgcttcg gccacagaag
     3241 ctcgaggagc agcgtgaagt acgcctccag acccttccgg acatagctgc gccctggcca
     3301 aagaaacagg ttcctggcga cctgttgggt gatggtgctt gctccccgga ggcggcccct
     3361 cgcatccttc tccagcaccg cgtcagcgat ggactcgaaa tcgaagcccc aatgataggg
     3421 gaatttttgg tcctcggccg cgaccacagc tagccgcatc tggggcgaga tggattccca
     3481 gccgatccag cggtagcgaa tgacaggctg gggttccctg cggagaatac tgccgacgcg
     3541 tttttcgagc atgaaggcac tcgtcggcgg tgcgatccag cgcagagaga ggaccggtgt
     3601 gaccgtgagg agaaccaggg caagtatggc acgccacatg agccggacga gtcggaagga
     3661 tttcctgttt ttcagcattg aggcctgcat gctggatgaa tcggtccgga agacatgcaa
     3721 aggtatcgct ctgcgcaccg cagaccgcac gctattacca ccgcaggggc gagtcaagat
     3781 gaaaggagag gaagtgaaag aagaagaatc cgcttgacgc aggcgcgtga caacgtcgaa
     3841 gataagcctc cgcgccgcat gctgcggtcg ggtgaaagga gtgtggccat ggcgatgttg
     3901 aagcacttgg agtccagggc caaggagatc ctggcgaggg aactgaaggt ttacgcagac
     3961 aagaacagca agtcccgaga ggcgtatgag cgatcctgcc gggtcctgcc gggcggtgtg
     4021 tcgcggacgc tgtgctactt cccgcccttc ccgtgccaga cggcgtacgg cgaggggtgc
     4081 tggatggtgg acgcggatgg gaaccggcgc ctggacctgt tcaactgcgc taccacgttg
     4141 atcctggggc accggccgcc ggcggtgatg aaggcggttc aggaacaact cacgcatggg
     4201 acggccttcc agacaaactc aggtctggaa acgaccctgg cggaaatgct ggtggagcgc
     4261 gtcgcctcgc tggagcagat gcgattcacc aacagcggaa gcgagggcac cctgatggcc
     4321 gtccgggcag ctcgggcttt caccggccgg cccaagatcg ccaagatgga ggggggatat
     4381 cacggcaccc acgatagtgt cgagatcagc gtgcgaccgc cgatggattt ggccgggtct
     4441 cccgaggaac ccaaggcggt ggctgggtcg gccgggatca ctcccggcac cgtgggggat
     4501 gtcgtcctcc tgccgtacaa caatcttcag gcggcccggg ccatcctggc cagggaggcc
     4561 tcccagatcg cggcgatcat cgtcgaaccg attctgggca gtgtgggctt cgttccggca
     4621 gaacgtgagt tccttcacgg gctccgggcc gaggcggaca agcacggaat gctcctcatc
     4681 ctggatgagg tgcagagctt ccgattggat cggggcgggg cgcaacagct cttcggtgtt
     4741 cgaccggacc tgacctgctt cggcaagatc atcggcggcg gattcccggt gggtgccttc
     4801 ggcgggcggc gggatgtcat ggcgctcttc aacactgcaa ccggcaaggg aaccattccc
     4861 cacgcgggga cgttcaacgg gaacccgatc accatgacgg ccgggatcgc gacgatggag
     4921 cagttgacgg aaccggtgta ctcgcggctc aacagcatgg gcgatcggct gcgggagcag
     4981 ctcgggcaga tcgggaggcg ccatgaggtt ccgatgcaga tcacgggcat cggctccatg
     5041 ttcaagattc acttcagcgc atcgcccgtc cgtgactacc ggtcaaccct ccaggccagt
     5101 cccttgctcc acactgcgct gttcctcttc agcttgaatc gcggcctgtt cctgtccgag
     5161 ggcggacgct gctgcgtgtc ggcggccatg ggtgacgccg aattgacgca gtacctgggt
     5221 acggttgagg agttcctggc agagctgacc cgctaaggca gccggggagc cgagaggcag
     5281 ggtgtatttc acacccctgc tcctcggcat ctctgcccca tgcggtttcc ctgctgcttt
     5341 tcggcagatc ctgcctgcgt tcgcaaccac agacaattga agaccacaga gccgattgcg
     5401 gttcaggttc agccccctga ggttgtatca ttgcggggta cgtgtccgaa catgtccatt
     5461 ggacagtgtg gacgtgataa cacgttcggc ttcaccgcgc agcaagcggg cgaactgaga
     5521 ggaaacatat gaccactgcg agtcgttcgc tggccgcgga ccttcgccgg ctgcttccgc
     5581 cggggcaggt cctggatacg cccgaagatc gaaccctgta cgcctatgac gggaccagca
     5641 gcagggccct gccggatgtc gccgtcatgg cgcgaagcgc gcaggacgta tcccacatcc
     5701 tgaagtacgc cacggcgcaa tccacgccgg tcgtgccgcg aggggccggc acggggcttt
     5761 ccggggggtc ggttcccgcc cgcgggggga tcgccctcgt cctcgcgtcc atgcgggcga
     5821 tcaaggagat cgataccggc agcatgctgg cggtggtgga gcctggggtg attacgggcc
     5881 agttccagaa gaccgtcgag gaggcgggga tgttctaccc gccggacccg gccagcctca
     5941 actactgcac cctcggcggg aacgtggcgg agaacgcggg aggcccccac ggggtgaagt
     6001 acggcgtcac caaggactac gttctgggtc tggaggtcgc cctgccgaac ggggacgtgg
     6061 cgcgacttgg cgggaagcag atcaagaacg tgaccggcta caacctggtc cagctcttcg
     6121 tgggatcgga gggcaccctc ggcgtcatca ccgagatcat cctgcggctc cttcccctgc
     6181 cgccggccaa gcgcaccatg ctggccatct tcgaccggct ggatgatgca gccacggccg
     6241 tggcggggat catggcgggc ggcgtgatcc cggcgaccct ggagatcatg gacaagaaga
     6301 gcatcaactg cgtggaggat tatctgaaac tggggttgcc tcgggatgcg gaggcgatcc
     6361 tgctgatcga ggtggacggg atgcaggccg cggtggaggc ggaggccgag gtcatcggcc
     6421 ggatctgccg cgaaaagggc gtgcggagct tccagacggc caaggacaag aaggaagagg
     6481 actccctctg gaaggcccgc cggtccatct cgccggccat atccgccgcg cggccaagca
     6541 agatcggcga ggacatcagc gtgccgcgga gcgccattcc cgccatggtg cgggagattc
     6601 aacgcatcgc caaggaatac gacctgccca tcgtgatctt cggccacgcc ggggacggca
     6661 atctccaccc gaacatcttg accgaccggc gggaccacca ggagatggag cgggtcgagc
     6721 aagccatcgc ggccatcttc gaggcgtcgg tcaaacttgg ggggacgctc tccggcgagc
     6781 acggcatcgg cttgagcaag caacagttcc tcaagctggc cctctccccg gaaaccgtca
     6841 agctaatggc ggacctcaag cgggccgtgg atccccaggg cattctgaat cccgggaaga
     6901 tcttcgggaa ggcatgatgg agctgacgca gctcggcaac ctcttcaagt gcaacaagtg
     6961 cgggtcctgc acgtcggtgt gccccctcta ccagcagacg gtgtacgagg gaatggccgc
     7021 gcgcgggaag ctggcgctcc tcgaggcgac ggtggaaggg aaactcgatt cgccccgggc
     7081 ggttcgggcg aagctc
//
LOCUS       DBNGZC010000654         1471 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_643542, whole genome shotgun sequence.
ACCESSION   DBNGZC010000654 DBNGZC010000000
VERSION     DBNGZC010000654.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_643542"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="AC16G0_11410"
     CDS             complement(<1..266)
                     /locus_tag="AC16G0_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100254.1"
                     /translation="MALWYVTSASPVAAADAPEGGTSLSYPAGIGLFVVPREILFYSA
                     QAGAWTSIKLDAGERVLRRASDGNVAAVVTSVRAIGFSAPLNVT"
     gene            complement(338..910)
                     /locus_tag="AC16G0_11415"
     CDS             complement(338..910)
                     /locus_tag="AC16G0_11415"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR5100255.1"
                     /translation="MVSDGTGLREARATRKAKGGRVDQATFQVRGEVRMPRKPGASSK
                     TRVASPRPTVPDGRRYARFAVRLPVRCRRLAARTTRTWSGRTADVGSGGFSVELPTRL
                     PPGTPLAVEIRTGIGPLRMEVEVRWTRCILGRVGIIRHGLCLADRSEILELPIGTLLG
                     EWLQGVAKREGKRGGRAHAHAQTQRRSKAR"
     gene            1196..>1471
                     /locus_tag="AC16G0_11420"
     CDS             1196..>1471
                     /locus_tag="AC16G0_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100256.1"
                     /translation="MRTAWVIAGVLATVLAATTTSADTVYLKNGEEIWGREVSEEGDM
                     VIIERPGGEVRVPKGEVSRIEHVQTSLPPYYAPPPPPPPPATGAPGSP"
BASE COUNT          265 a          469 c          488 g          249 t
ORIGIN      
        1 tcggtcacat tgagaggcgc actgaagccg atcgcccgca ccgaggtcac gacggccgcg
       61 acgttgccgt ccgacgcgcg ccggagaact cgctcgccgg catccaactt gattgaggtc
      121 caggccccgg cctgggcgct gtagaaaagg atctcgcgcg gaaccacaaa caggccgatg
      181 cctgctgggt atgataggct cgtccccccc tcgggggcat ctgcggcggc cacgggggaa
      241 gccgatgtca cataccagag tgccacgagg aggaagagac cggcgagtcc aaaatggcgt
      301 gggatcctac ggtttccggg gatgaagcgg acgagcatca tcgggctttg ctccgcctct
      361 gggtctgggc gtgggcgtgt gcccggccgc ctcgcttccc ttcgcgcttg gccacaccct
      421 ggagccactc tccgaggagg gtaccgatgg gaagttccag gatctccgag cgatcggcca
      481 ggcaaagtcc gtgacggatg atgccaaccc gaccgaggat gcagcgcgtc caccgcacct
      541 cgacttccat ccgaagcggg cctatacctg tccggatctc tactgccaga ggggttccag
      601 gtggcaaccg ggtggggagt tccacgctaa atccgccgct gcccacgtcg gcggtccggc
      661 cgctccaggt ccgggttgtg cgagcggcca ggcgcctgca gcggacgggg agccgcacag
      721 cgaatcgagc gtatcgcctg ccgtcaggga ccgtagggcg gggcgacgcc acgcgcgtct
      781 tggaggaagc tcccggcttc cgcggcatcc tgacttctcc tcgcacctgg aacgttgctt
      841 ggtccacgcg gccccccttg gctttccgag ttgcccttgc ttctctgagg ccagttccat
      901 cagaaaccat ccctcgaccg cattctctag tgcttttaac gggcaaagtc aatacctggg
      961 ggccgcgggg aaagcatggg agaagggacc tgtccccgtc tcgcggcgaa aacccttgac
     1021 gctcgtggcg ggcgcatcta gattcagcat gcggcacggg ggagattgtg atgcgtcctt
     1081 tttcctcatg atcatacggc aaggactcag ggtccacgga ctctccgggt tcgcgaaagt
     1141 ttctacttca cgcgatcgcc agaagaggac gaccggacac aagggaggac ggctgatgcg
     1201 cacggcctgg gtgattgctg gggtgctggc gacggtcctg gcagcgacca cgacgagtgc
     1261 ggacacagtg tatctcaaga atggcgagga gatctggggg agggaggtat ccgaagaggg
     1321 ggatatggtg atcatcgagc gtccgggagg agaggtccgc gttccaaagg gggaggtcag
     1381 ccggatcgag catgtccaga cgtcgctccc accgtactac gcaccgcccc cacctccccc
     1441 tccccctgcc accggggcgc ccggctcccc c
//
LOCUS       DBNGZC010000655         1721 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1524758, whole genome shotgun sequence.
ACCESSION   DBNGZC010000655 DBNGZC010000000
VERSION     DBNGZC010000655.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1524758"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..1101
                     /locus_tag="AC16G0_11425"
     CDS             199..1101
                     /locus_tag="AC16G0_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020875852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5100257.1"
                     /translation="MKSDSLLLLAACIWGFAFVAQRLGMQHVGPFGFNAVRFALGCLV
                     LLPLLFRSDIRHAGESSPSAGLFSLHRLGGGLLAGVVLFAGASFQQVALVYTTAGNAG
                     FITGLYVILVPIIGIALGHRTQAGTWVGAALAAVGLYLLSVTDQFAVSPGDVLVLIGA
                     FFWACHVHIIGWLSPRQDPLRLACLQYAACSLLSLIVSVATEQNTLSRYLSAAIPILY
                     GGVMSVGVAYTLQVVAQKQAKPSHAAIILSLEAGFAAMGGWIILGEILTRRAMMGCIL
                     MFCGMLTSQLWRPPLREARLLRAD"
     gene            1262..>1721
                     /locus_tag="AC16G0_11430"
     CDS             1262..>1721
                     /locus_tag="AC16G0_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen
                     decarboxylase/cobalamine-independent methonine synthase
                     family protein"
                     /protein_id="HIR5100258.1"
                     /translation="MNKRETVLSLLDSGSTPGYTPAAFFLHFDPMYHRGQAAVDKHLE
                     YFRYTGMDLVKIQYERTFPHLPEIRRPADWAQMPCYGLEFYEAPLGVLAGLVKAAKSE
                     ALVIQTLYSPFMCAGHTVGAEILADHIRQDPERVKRGMQVITDSLLRFVKE"
BASE COUNT          279 a          569 c          521 g          352 t
ORIGIN      
        1 gaccccagac ccgcggctgt tacgccgcgc gactggggtc cgcccttggt attcggggga
       61 gaccattaac tatcggtgag acactcaaat tcaatggtcg ggacaggaag gggcccatcg
      121 ggatcagaat aaaccggggc cgggtatctt gtgatgagag agcatcccat ggcacggacc
      181 gccaagggtg gccatccagt gaaatctgac agcctcctcc ttctcgccgc ctgcatctgg
      241 ggtttcgcct ttgtggccca gcgtctcggc atgcagcacg tgggcccctt cgggttcaac
      301 gctgtgcgct ttgctctggg ctgcctcgtg cttctgccct tgctcttcag gagcgacatt
      361 cggcacgctg gcgagtcatc gccgagtgcg gggctgttct cgctccatcg cctcggcggg
      421 ggcctcctgg ccggagtcgt cctctttgcc ggagcatcct tccagcaagt ggccctcgtc
      481 tacaccaccg caggcaatgc gggtttcatc acggggctct atgtgatcct cgtacccatc
      541 atcgggatcg ccctcggcca caggacgcag gccggcacct gggtcggcgc cgcgctggct
      601 gccgtcggcc tctacctcct gagcgtcacc gaccagttcg ccgtgtcgcc gggcgacgtc
      661 ctggtcctca tcggcgcctt cttctgggcc tgccatgtgc acatcatcgg ctggctctcg
      721 cccaggcagg acccactgcg cctcgcctgt ctgcagtatg ccgcctgttc tctgctcagc
      781 ctgatcgttt cggtcgccac tgagcagaat acgctgagcc gctatttgtc tgcggccatc
      841 cccatcctct acggcggcgt catgtccgtg ggtgtggcct acaccctcca ggtggtggcc
      901 cagaagcagg ccaaaccctc acacgctgct atcatcctga gtctggaggc gggctttgcc
      961 gccatgggag ggtggatcat ccttggcgag atcctgaccc gccgggcgat gatggggtgc
     1021 atcctcatgt tctgcggtat gctcacctcc caactctgga gacccccgct gagggaagca
     1081 cgtctcctcc gtgccgattg agcatgcagg cggacccgaa ttctcggcac tggcgagcac
     1141 gacataccgg aggaaatggc gcgacccgca ggcctgcgtt cccccgattt tttgcccgct
     1201 gtcgtctcgg ctggacctcg gaggccgcat gacgtccgtt cgtccacagg gaggagtgca
     1261 tatgaacaag cgcgagacag tcttgagcct gttggattcc gggtccacgc ccggatatac
     1321 cccggcagcc ttcttcctgc atttcgaccc gatgtaccac cgcgggcagg ctgccgttga
     1381 caagcacctg gagtacttcc ggtacacggg catggacctg gtcaagatcc agtacgaacg
     1441 caccttcccg cacctgccgg agatccgacg gcctgcggac tgggcacaga tgccctgcta
     1501 tgggctggag ttctacgaag cgccgctggg cgtgctggcg gggctggtca aggcggccaa
     1561 gtccgaagcc ctggtgattc agactctcta ttccccgttc atgtgcgccg gacatacggt
     1621 cggtgccgag attctcgccg atcacatccg ccaagacccg gaaagggtga aacgcggcat
     1681 gcaggtgatc accgacagtc tgctgcgctt cgtcaaagaa t
//
LOCUS       DBNGZC010000656         2243 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2405444, whole genome shotgun sequence.
ACCESSION   DBNGZC010000656 DBNGZC010000000
VERSION     DBNGZC010000656.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2405444"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1533
                     /locus_tag="AC16G0_11435"
     CDS             <1..1533
                     /locus_tag="AC16G0_11435"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HIR5100259.1"
                     /translation="SSSAAQLVPDMVLAGIEARFQSTGEPKGLTLVFPVAVGDSFGTA
                     GMDHLAHPGLIQRLIGGSYVNGPASKPAPRIYAMILDEQVEAYNFPLGVLMHLHRDIA
                     ARKPGVITKIGLGTFVDPRDQGGRMNRLTPPDLVEVIQLGGEEWLLYRAFPIDVAIIR
                     GTTADTHGNLTLEHEGVVLSILAQAMAAHNSGGKVIAQVKRVAAPGTLDPQKVKVPGI
                     LVDALVVDPEQRQNTGIAYDPAISGEIRTAQFPVEPLPLGPEKVIARRALCQLRPGDI
                     VNLGFGISSLVPQVAFEEGVFDRLTFTVEQGAIGGLPLTGFAFGASHNPEAIIDSAAQ
                     FDLIDGGGVTVGCLAFAEVDGQGNVNVSRLAAQPHVLAGAGGFINIAQGTRKLLYCGT
                     VTAGGLDVRVENGQIRIVRDGRFPKFVARTQHVTFNGAMAAKAGQEVWYFTERGVFLL
                     TPEGLVLTEVAPGIDVEKDIRARVEFPVRLAPEVRLMDPRLFRAAPMGLDVAWREDSR
                     PG"
     gene            1545..1994
                     /locus_tag="AC16G0_11440"
     CDS             1545..1994
                     /locus_tag="AC16G0_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017248425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR5100260.1"
                     /translation="MERHYEDMQVGEEYESPARTITETDIVNFSAISGDWSPVHSDEE
                     YCKHSPYKTRIAHGLLGLAISEGLKFRIPEFANARYMASLYWNYKFTGPILMGDTIRV
                     RVKVLSKRETKKPDRGIVVEYVTMLNQREEIVQEGEHGLMVLRKGVA"
     gene            2024..>2243
                     /locus_tag="AC16G0_11445"
     CDS             2024..>2243
                     /locus_tag="AC16G0_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003329589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5100261.1"
                     /translation="MNFELSDAQRMLRDMVREFAEKEIRPRAAEIDRTDEFPWDLWRR
                     MAELGILGMTLPTEYGGSGMDTLCWAIAQ"
BASE COUNT          397 a          698 c          783 g          365 t
ORIGIN      
        1 agcagttcgg cggcgcagct cgtgccggat atggtcctgg ccggcatcga ggcgcgcttc
       61 cagtcgacgg gggaacccaa ggggctcacc ctggtctttc ccgtggcggt gggggactcc
      121 ttcggcactg cggggatgga ccatctggcg caccccgggc tcatccagcg gctcatcggg
      181 ggctcctacg tgaacggccc cgcgtcgaaa ccggcgccca ggatctacgc catgatcctc
      241 gacgagcagg tggaggcgta caattttcct ctgggagtcc tgatgcacct ccaccgcgac
      301 atcgccgcga ggaagccggg cgtaatcacc aagatcggcc tggggacgtt cgtggacccg
      361 cgggatcagg gcggacgcat gaaccgcctg acgccgccgg atctggtgga ggtgatccag
      421 cttgggggcg aggagtggct cctctaccgc gccttcccga tcgacgtggc catcatccgg
      481 gggaccacgg ccgacaccca cggcaacctg accctggaac atgagggcgt tgtcctgtcc
      541 atcctggccc aggcgatggc agcccacaac tccggcggga aggtgatcgc gcaagtgaag
      601 cgggtggcgg cccccggaac gctcgatcca cagaaggtga aggttcccgg aatcctggta
      661 gacgccctgg tcgtggatcc ggagcagcgc cagaacaccg gcatcgccta cgaccccgcc
      721 atcagcggcg agatccggac ggcacagttt ccggtggagc ccctgcccct ggggccggag
      781 aaagtcattg cgcgccgggc cctctgccag ctccggcccg gcgacatcgt gaacctgggc
      841 ttcggcatct cctctttggt gccccaggtt gccttcgagg agggggtatt cgaccggttg
      901 accttcaccg tggagcaggg ggccatcggc ggcctgccgc tcaccggatt cgccttcggg
      961 gcctcgcaca acccggaagc catcatcgac tcggccgccc agttcgacct catcgacggg
     1021 ggcggggtga cggtggggtg cctggccttc gccgaggtgg acggccaggg caacgtcaac
     1081 gtcagccgcc tcgcggccca gcctcacgtc ctggcgggag caggcgggtt catcaacatc
     1141 gcccaaggca cgcggaagct gctctactgc ggcaccgtga cggccggcgg ccttgacgtg
     1201 cgtgtagaga acgggcagat ccggatcgtc cgggatggac gcttcccaaa gtttgtggcc
     1261 cggacgcagc acgtgacctt caacggcgcc atggcagcca aggcaggaca ggaggtctgg
     1321 tacttcacgg agcggggggt cttcctgctg acgcccgagg gcctggtgct cacagaggtt
     1381 gccccgggca tcgacgtgga gaaggacatc cgggctcgcg tggagttccc cgtacgcctg
     1441 gcgccggagg tccgtcttat ggacccccgt ctcttccggg cggcgcccat gggccttgac
     1501 gtggcgtggc gggaggacag ccgtccgggc tgaaaggagc gaagatggaa cggcactacg
     1561 aggacatgca ggtgggggaa gagtacgagt ctccggcccg caccatcacc gagacggaca
     1621 tcgtgaactt ctcggcgatc tcgggagatt ggagcccggt ccattcggat gaggagtact
     1681 gcaagcactc cccctacaag acccggatcg cccacggctt gctggggctg gccatttctg
     1741 aaggcttgaa gttccgtatc ccggaatttg ccaacgcgcg gtacatggcg tccctctact
     1801 ggaactacaa gttcacgggg ccgatcctga tgggagacac catccgggtt cgagtcaagg
     1861 tcctcagcaa gcgcgagacg aagaaaccgg accggggaat cgtggttgag tacgttacca
     1921 tgctcaacca gcgggaagag atcgtgcagg aaggggagca cggcttgatg gtcctgcgga
     1981 aaggcgtggc ctgagatcga tcatttccag gcgaggaggc cgcatgaatt tcgagctgtc
     2041 ggacgcacag cggatgctgc gggacatggt ccgggaattc gcggagaagg agatccggcc
     2101 tcgtgcggcc gagatcgacc ggaccgatga gttcccgtgg gacctctggc gccggatggc
     2161 ggagttgggc atcctgggga tgacgctccc gacggagtac ggcggctcgg gtatggacac
     2221 gctctgctgg gccatcgcgc agg
//
LOCUS       DBNGZC010000657         1858 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1918678, whole genome shotgun sequence.
ACCESSION   DBNGZC010000657 DBNGZC010000000
VERSION     DBNGZC010000657.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1918678"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..264
                     /locus_tag="AC16G0_11450"
                     /pseudo
     CDS             70..264
                     /locus_tag="AC16G0_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_217639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1249..>1858
                     /locus_tag="AC16G0_11455"
     CDS             1249..>1858
                     /locus_tag="AC16G0_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020393465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HIR5100262.1"
                     /translation="MVGSKPKVKSFEIDKRLVHEAWMKVQANGGAPGVDAVSIGGFRE
                     AERDNLYKLWNRMSSGSYLPGPVRAVEIPKDHGAGIRVLGVPNVADRIAQTAAAMLLE
                     EKLEPIFHPDSYGYRPGRSAHDALAVTRRRCWKQDWVLDLDVRAFFDSVPHDLLLKAV
                     AHHTDERWVLLYIQRWLKAPMQMPDGTLVARDKGTSQGSPISP"
BASE COUNT          355 a          596 c          630 g          277 t
ORIGIN      
        1 gccgccctgg tggccgccga ggcccgccgc cgcggcgccg accacatccg ccaactcgtg
       61 gtcctcggtg acggggcgcc ctggatctgg aacctggcca ccgcgatcct gcccgaggcc
      121 acccccatcg tggacctcta ccacgcccgc gaacacctgc acgacctcgc cgcgcacctg
      181 agcccggtcc taggcgacgc ccaccccgag tggctggccg cccgccgggc cgacctcgat
      241 gccggcgaca tcgaaaccct cctcaccgcc accgaacggc tcctgccgca gctgcccgac
      301 gacaccgcga acaacaccgc caaagcattg gggtacttca agaccaatgc ccaccggatg
      361 cgctatgcct acttccgcga gcacggcctg ttcgtcggca ccggcgtcgg cgaggccggc
      421 tgcaaatccc tcgtcggtca acgcctcaaa ctgtccggca tgcgctggaa catcccgggg
      481 ccaccgccat cctcaccctg cgctgccacc aggccagcgg ccgcttcgac cacatctgga
      541 cacagccgca caaccagacc gccacctacg ccaccgcatg accagcaggc cggcggttca
      601 attcaagatc aacttcggtt acctacgtgt gtcctgaaag tgaagtgagg aggaggtaga
      661 agatcctcag cacgaagcgt gctgtgccag cgatggaggt aggtcctccg ggttcgccga
      721 gcagacggag aactcaaacc gccgcagtgt catgccggtc aagagccggg gtgggaagcg
      781 actgcggaaa aggcccgctg cagcgggtca ggtatgcgac gagaagacga acatggatga
      841 accactgctg acgcatcgaa acgtagaacg atggcgccga aaccggaggt tgtgggtatc
      901 tccgggacaa aggcccgccc gtggggcgag ccctgagcca gggggcgggc tgcgtgctgc
      961 cctggcggcg tccggtgtat aggtggcgtg agctcgtcgc gggcgctggc atggaacagg
     1021 agaacctgtc gcctcgatac cgacgaccgg atgatatcgg cgcggtgtgc cggttctggt
     1081 cgcgagaggg agaaccccaa gtggccgaaa ccgcaagggg cagagtaccg atgcgaggca
     1141 caggggcgga ccggctcgta gtagcggtga aggctctgta atggggctgg agcgaagggg
     1201 ccgggcgggc cagatcgacc tgaggtcacc ccgcacgggt aggagctgat ggttggatcg
     1261 aagccgaagg tgaagtcgtt tgagatcgat aagcgcctcg ttcatgaggc gtggatgaag
     1321 gtccaggcca acggcggagc gccaggcgtg gacgccgtca gcatcggcgg gttccgggag
     1381 gcggagaggg acaacctcta caagctgtgg aaccggatga gctccggcag ttacctgccg
     1441 ggcccggtgc gggcggtgga gataccgaag gatcatgggg cggggatcag ggtgttgggt
     1501 gtgcccaatg tggccgaccg aatcgcccag accgcggcgg cgatgctgct ggaggagaag
     1561 ctggagccga tctttcaccc cgacagctac ggttaccgtc cggggcgttc ggcccacgac
     1621 gcgttggccg tgactcggcg gcgctgctgg aaacaggact gggtgttgga ccttgatgtc
     1681 cgggcctttt tcgacagcgt tccccatgat ctgctactca aggcggtcgc tcaccacacc
     1741 gacgagcgat gggttctgct ctacatccag cggtggctga aggcccccat gcagatgccg
     1801 gacgggaccc tcgttgcacg agacaaggga acctcacagg gctccccgat ttcgccgt
//
LOCUS       DBNGZC010000658         2017 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2113176, whole genome shotgun sequence.
ACCESSION   DBNGZC010000658 DBNGZC010000000
VERSION     DBNGZC010000658.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2113176"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..923
                     /locus_tag="AC16G0_11460"
     CDS             18..923
                     /locus_tag="AC16G0_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5100263.1"
                     /translation="MAWPAYLGLHRTGELRRRSEAARQVLAACRLCPRECKVRRLEGE
                     TGVCGAGSQAVVSSYGPHFGEEAPLVGSGGSGTIFLAHCNLCCVFCQNFEISQRRDGR
                     EVCPHELAQMMLELQRQGCHNINFVTPTHEVPQILQALILAVDGGLRLPLVYNSGGYE
                     SLETLRLLDGVMDIYMPDFKYGDAGIGKRYSGVENYPEVARAALREMHRQVGDLALDE
                     RGIATRGVLVRHLVLPNGLAGTPDVARFLADLSKDMYVNLMNQYHPRYRAHEEPLLSR
                     RITRKEFDEALSSARDAGLHRLDGY"
     gene            973..>2017
                     /locus_tag="AC16G0_11465"
     CDS             973..>2017
                     /locus_tag="AC16G0_11465"
                     /inference="COORDINATES: protein motif:HMM:NF026066.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR5100264.1"
                     /translation="MKNAEIKDLFNEIADFLEIKGENPFRVRAYRRAAQAVEGLSEDI
                     AAIAGRGALVTIPGIGKDLAAKIQEYLDSARVEYLEDLRREIPAGVIELMRIHGVGPK
                     TARLLYEQVGVDSVEKLETLALAHNLAGLPGIKAKTEENILKGIQVWKSGRERMPLGS
                     ALVLAEGILQALRALHEVDQIAVAGSLRRMKETVKDIDLLVTSAKPARVMEVFVGLPN
                     VAEVLAHGDTKSSVRLKEAIQVDLRVVDPGCFGAALQYFTGSKQHNIRVRELAQRKGL
                     KVNEYGVFQEGSDRRVAGATEEEVYRAIGLPLIPPELREDGGEIEAAMEGRLPTLVSP
                     DDIRGDLQLHTTWS"
BASE COUNT          365 a          612 c          708 g          332 t
ORIGIN      
        1 gtcatcgagg agaagatatg gcctggcccg cctacctggg gctccaccgt acgggtgagc
       61 tgcgccgacg cagcgaagcg gcgcggcagg tcctcgcggc atgtcgtctc tgcccgcggg
      121 agtgcaaggt tcgacgcctg gaaggtgaga ccggcgtgtg cggcgccggc agccaggcgg
      181 tcgtctccag ttacggcccg cacttcggcg aggaggcgcc cctggttggc tccgggggtt
      241 cgggaacaat ctttctggct cactgcaacc tctgctgcgt cttctgccag aacttcgaga
      301 tcagccaacg gagggacggg cgggaggtct gtccccacga gctcgcccag atgatgctgg
      361 agctgcagcg gcagggctgc cacaacatca atttcgtgac gcccacccac gaggtgccgc
      421 aaatcctgca ggccctgatc cttgccgtgg atggcggcct ccggcttccc ctggtgtaca
      481 actccggggg ctacgagtcc ctggagaccc tgcgtcttct cgacggggtc atggacatct
      541 acatgccgga cttcaaatac ggggacgcgg gaatagggaa acgctattcc ggggtggaga
      601 actatccgga ggtcgcgcga gccgccctgc gcgagatgca ccggcaggtc ggagacctcg
      661 cgctggatga gcggggcatc gccactcggg gggtcctggt gcgccacctg gttttgccca
      721 acggacttgc cgggacgccc gacgtcgcgc ggttcctcgc tgatctctcg aaagacatgt
      781 atgtgaacct catgaaccag tatcatccca ggtaccgggc ccacgaggag cccctcctga
      841 gccgccggat cacccggaag gaatttgacg aggcgctcag ctcggcccgc gacgccggcc
      901 tccaccgtct ggatgggtat tgatgagaga agccgtctgc cggcagccct cggccctcag
      961 ctttggaata ggatgaaaaa cgccgagatc aaggatctgt tcaacgagat cgccgacttt
     1021 ctggagatca agggcgagaa cccgttccgc gtgcgtgcct accggcgtgc ggcgcaggcc
     1081 gtggaggggc tctccgagga catcgcggcg attgcgggtc ggggtgcgct cgtcacgatt
     1141 cccgggatcg gcaaggacct cgcggccaag atccaggagt acctcgacag tgcgagggtc
     1201 gagtacctgg aggatcttcg gcgggagatc cccgccgggg tgatcgagct gatgcggatc
     1261 cacggggtcg gacccaagac ggccaggctg ttgtacgagc aggtcggcgt ggattccgtg
     1321 gagaagttgg agacgctggc cctggcccac aatctggccg ggctgccggg gatcaaagcc
     1381 aagacggagg agaacatcct caagggcatc caggtctgga agagcgggcg ggaacggatg
     1441 cccctggggt cagccctggt gctggccgaa ggaatcctgc aggctcttcg agcgctccac
     1501 gaggtggacc agattgccgt ggccgggagc ctccgccgga tgaaggagac ggtgaaggac
     1561 atcgatctcc tggtcacctc ggcgaagccg gcgcgggtca tggaggtgtt cgtcggcctc
     1621 ccgaacgtgg ccgaggtgct ggcccacggc gatacgaagt ccagcgtccg cctcaaggag
     1681 gcgatccagg tggacctgcg cgtggttgac cccggctgct tcggcgcggc gctccaatat
     1741 tttaccggct ccaaacagca caacatccgg gttcgcgagc tggcccagcg aaaagggctg
     1801 aaggtcaacg agtacggcgt cttccaggag gggagcgaca ggcgggtggc gggcgcgacc
     1861 gaggaagagg tgtatcgagc catcggcctc cccctaatcc cgccggaact ccgtgaggac
     1921 gggggtgaga tcgaggccgc catggagggt cgattgccga ccttggtgtc cccggatgac
     1981 atccggggcg acctgcagct acatacgacc tggagtg
//
LOCUS       DBNGZC010000659         2540 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2500788, whole genome shotgun sequence.
ACCESSION   DBNGZC010000659 DBNGZC010000000
VERSION     DBNGZC010000659.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2500788"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            285..752
                     /locus_tag="AC16G0_11470"
     CDS             285..752
                     /locus_tag="AC16G0_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100265.1"
                     /translation="MVGLYGAVCALSPSTPRFLDRVDLVFHEAGHLLFAAFGEFIGVL
                     GGSLMQLLIPAIIVGYFFFHQQLYSGAVSLFWLAQSLFNVSVYVRDARTQALPLLGGE
                     DTLHDWHYILGRLRLLNWDQMLGNLAYLVGLATLIASVLGGLYYSLEEEHDAG"
     gene            1086..2054
                     /locus_tag="AC16G0_11475"
     CDS             1086..2054
                     /locus_tag="AC16G0_11475"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase family protein"
                     /protein_id="HIR5100266.1"
                     /translation="MKAILFDQVGGPEVLRVGEAPKPEILPGMVLIKNHAIGVNFADV
                     LFRRGTYLSQPSFPDTPGLEAAGVIEAVGPGVEDLKVGMRVAAVTTKTYAEYTLAKAS
                     QTFVLPAMMSFERGAAFPIQTLTAYHMIHTAHQTTPRQTVLIHSAAGGVGGLAVQIAR
                     AAGARVIGTISDDGKTALVKENGADLVINYVTHDFAEEALRFTDGKGVNLILDAVGKP
                     TLERDFRCLAPLGRLILYGRAGGSPDPFNLLSLLGKSHTVSGFVLYTIFDMPEKKLEG
                     VVHSLQLMQEGKLRLRIGKTFPLAEAAEAHRYLESRQSAGKLVLIP"
     gene            2163..>2540
                     /locus_tag="AC16G0_11480"
     CDS             2163..>2540
                     /locus_tag="AC16G0_11480"
                     /inference="COORDINATES: protein motif:HMM:NF016156.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HIR5100267.1"
                     /translation="MDRVFFTLGAWMAALGMAAGAFGAHILRVRVAPEMLAAFETGVL
                     YQLVHALALIGVAWATTRWPGRATVIAGWLFLAGTLLFSGSLYALALSGAFFFGAVTP
                     LGGLALIAGWLVLAWAAWQERLPT"
BASE COUNT          463 a          773 c          814 g          490 t
ORIGIN      
        1 atcagcagtc agcaaagact caacacgggg gcatgtccgg aagagcgggc atgcccccgt
       61 tgcatctgtg acgggatggg cgtaggatag ccgctgtctg ccgagcaaag ccgggaccac
      121 gccgcgcagg gttcacggcc caaggttcct gcgagcctgc gaacctcgat cgggggacag
      181 cttgaaatca ggaaggcggc atgaagcaga ggccagcggg acctgaagaa accgagcctt
      241 cctcgggcag acgcctcaat tgtctcaagc ttggtttcct actcatggtt gggctctacg
      301 gggctgtgtg tgcgctgagc ccctcgaccc cccggttcct ggatcgcgtg gacctcgtct
      361 ttcatgaggc aggacacctg ctctttgctg cgttcgggga gttcattggc gtcttgggcg
      421 gatctctaat gcagctactc attcccgcga tcatcgtggg ctacttcttc ttccaccaac
      481 agctctactc cggcgcggtc agcctgttct ggctggcgca aagccttttc aacgtctccg
      541 tgtacgttag agacgccaga acacaggccc tgccgctcct gggtggcgaa gacaccctgc
      601 acgactggca ctacatcctc ggccgtctcc gcctcctgaa ttgggaccag atgctgggga
      661 atctggcata cctcgtgggc ctcgccaccc tgatcgcgtc tgtcttgggc ggtctctact
      721 actccctgga ggaagaacat gacgcggggt agcttctctc caccccactg atccccgcag
      781 atccccttct tccaaaaacc ccttgtgttc ctcgtggacg catgcataga atcctcccgc
      841 caatccctcg aattcataat cctaccaggt gcggccatga acatggctcg agtgaagatc
      901 catccggaga gaggcggcac gtccagaaat tctcaggtag ctacccgagg atcgccggga
      961 ttcatcgcca cccccctgga ttcggggacg acgggaaaca gggcggagag ccagcggacc
     1021 ttaatgtggg gtgacggtta ggaccagtac gtcggcatgg ctcattaacc aagggggaca
     1081 actgcatgaa agccattctg ttcgaccagg tcggtgggcc ggaggtgttg cgggtggggg
     1141 aggcgccgaa gccggagatc ctgccaggga tggtgctgat caagaatcac gccatcgggg
     1201 tgaacttcgc cgacgtgctc ttccgccggg gaacgtactt gagtcagccg agcttccccg
     1261 atacgccggg tttggaggcc gccggcgtga tcgaggcggt aggacccggc gtggaggacc
     1321 tgaaggttgg gatgcgggtg gccgcagtga ccaccaagac ctatgcggaa tacacgctgg
     1381 caaaggcctc tcagaccttt gtgcttcccg ccatgatgag cttcgagagg ggcgcggcct
     1441 tcccgatcca gacgctcacc gcgtaccaca tgatccacac ggcccaccag acaaccccca
     1501 ggcaaacggt gctgatccat tctgccgccg gtggcgtggg cggcctggcg gttcagattg
     1561 caagagccgc gggcgcgcgg gtgatcggga cgatttccga cgacggcaaa accgcactcg
     1621 tcaaggagaa cggggccgat ttggtgatca actatgtgac ccacgacttc gccgaggagg
     1681 cgttgcggtt caccgacggc aaaggggtga acctgattct tgacgcggtg ggcaaaccaa
     1741 ccctggagcg cgacttccgg tgtctcgcgc ctctcgggcg cctgatcctg tacggtcggg
     1801 ccggaggctc gcctgatccg ttcaatctgc tcagcctgct ggggaagtcc cacacggtga
     1861 gcgggttcgt cctctacacg attttcgaca tgccggaaaa aaagctggaa ggcgttgtcc
     1921 actctctcca gctcatgcag gaaggcaagc tccggctccg catcggcaag actttccccc
     1981 tggcggaagc cgctgaggct caccggtacc tggagtcccg tcaatctgcg ggaaagctgg
     2041 tgctgatccc gtgatggaga gcccagagcc gcgagccacg agccacgaac gggagaacac
     2101 accgcttatg cagagccgtg agccgcgagc gggatagcac aacaccagat gagggcggag
     2161 gaatggatcg ggtcttcttc acgctgggcg cgtggatggc agctttgggg atggccgcgg
     2221 gtgcctttgg tgcccacatc cttcgggtac gggtcgcgcc ggagatgctg gctgcctttg
     2281 aaacgggcgt gctctaccag ctggtgcacg ccctggcgtt gatcggcgta gcctgggcta
     2341 cgacccgctg gccgggaaga gctacggtca tcgcgggctg gctgtttctc gcgggcacgc
     2401 tcctattttc tggcagcttg tacgcgcttg ccctgagcgg ggctttcttc ttcggggcgg
     2461 tcacgcctct cggcgggctg gccctgatcg cgggctggct ggtccttgcc tgggctgcgt
     2521 ggcaggagcg cttacccacg
//
LOCUS       DBNGZC010000660         2980 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1621238, whole genome shotgun sequence.
ACCESSION   DBNGZC010000660 DBNGZC010000000
VERSION     DBNGZC010000660.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1621238"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="AC16G0_11485"
     CDS             complement(<1..786)
                     /locus_tag="AC16G0_11485"
                     /inference="COORDINATES: protein motif:HMM:NF004616.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HIR5100268.1"
                     /translation="MAAPMLSEVVFRVHRFDPERDSHPHYEDYRLSVKPGATILDGLW
                     KIKEGQSPTLSWRFSCRMGVCGSCGMLINGSPRLACNTQIAELGTPIIAVAPLPNFDI
                     IRDLVPDLLPMFKTHQELRPYILREDTDECENPTGEFRQTPEELEHYLQFSYCIKCGC
                     CMAACPTMASDLEYSGPMPLAQAHRYNEDNRDSGFQTRKQELAFGRGPWRCHYAGECS
                     RVCPKGVDPAKAIQYMKRALVLDFLHLRKMGCPAKLAEQPKDLK"
     gene            complement(796..1170)
                     /locus_tag="AC16G0_11490"
     CDS             complement(796..1170)
                     /locus_tag="AC16G0_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     transmembrane subunit"
                     /protein_id="HIR5100269.1"
                     /translation="MRDQKLWTWHVAAGVLVVVLLGLHMVVMHLSRTVGIGNPAGGHP
                     VDWGNVVARGKSALFTISYILLLGAALFHGLYGFRNIVGEIGPSAGLKKTINALLLVG
                     GLGLFGLGTWAAIASFQLAKSL"
     gene            complement(1183..1620)
                     /locus_tag="AC16G0_11495"
     CDS             complement(1183..1620)
                     /locus_tag="AC16G0_11495"
                     /inference="COORDINATES: protein motif:HMM:NF013307.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     transmembrane subunit"
                     /protein_id="HIR5100270.1"
                     /translation="MFTTKHYPNRLGVWGWLGGGRWGFERYLYSLHRLTGLGLLAYFL
                     LHIVLTTSRTLGPEAWARAMKTVEGPIFTLGEYLVFAAFVIHGLNGIRLVLLELGVGV
                     GKPIEPVYPYRTSVHVQRPLAVGVMVLAAVAVVIGGLDFFILK"
     gene            complement(1727..>2980)
                     /locus_tag="AC16G0_11500"
     CDS             complement(1727..>2980)
                     /locus_tag="AC16G0_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR5100271.1"
                     /translation="FTRYDEFFVTDILVEDGRFAGLLGIHAASGETVLLQGKALLIAT
                     GGGGSLYGFTTYSQTVSGDGMAMAYRAGLPLEDMEFLQFHPTGLVPSGILMTEACRGE
                     GGYLRNSKGERFMEKYAASKMELAPRDMVSRAEMTEILEGRGFPHPDGLDYVHLDLTH
                     LGAEKINTRLPLIREVCIKFIGLDPIEKPIPVRPVAHYSRGGVDCDSNGRTRLPNVWV
                     AGEAACVSLHGGNRLGSNSTAECLVWGGICGEEIVKALPRLKVASTSEARVREGRRHV
                     EEIVNRTGNENLYQVKQDLRSTMDKYVGVYRKGVELTEALKRIGEIRERFRRAPVDDK
                     SKVYNSNLFNALELENLIDLAEVTVAGALERKESRGAHARRDFPTRDDQEWLKHTLAF
                     RDEAGPKLSSKPVTINTWKPVERKY"
BASE COUNT          518 a          989 c          925 g          548 t
ORIGIN      
        1 cttcaggtcc ttgggttgct cggccagctt ggctggacaa cccatcttcc ggagatgcag
       61 gaagtccagg actagggctc gcttcatgta ctggatggct ttggctgggt cgacaccctt
      121 cggacacacc cgggagcact ctcctgcgta gtggcaacgc caggggccgc ggccgaaggc
      181 cagctcctgc ttccgtgtct ggaagccgct gtcccgattg tcctcattgt agcggtgtgc
      241 ctgggcgagc ggcatgggac ccgagtactc cagatcggac gccatggtgg ggcatgcggc
      301 catgcaacag ccgcacttga tgcagtagct gaattgcagg tagtgttcca gctcctcggg
      361 ggtctgcctg aactcccccg tggggttctc gcactcatcc gtgtcctcgc ggagaatgta
      421 cggccgaagc tcctgatgcg tcttgaacat cggcaggaga tccggaacca ggtcccggat
      481 gatgtcgaag ttcggcagcg gcgccaccgc gatgatgggc gtgcccagct ccgcgatctg
      541 ggtgttgcag gccaggcgcg gagagccatt gatcagcatg ccgcaggagc cacacacacc
      601 cattcggcag gagaatcgcc aggacaaggt cggcgactgg ccctccttga tcttccagag
      661 gccgtcgagg atcgtcgcgc ccggcttgac cgagaggcgg tagtcctcgt aatgggggtg
      721 cgaatcgcgc tcgggatcga aacgatgaac ccgaaagacg acctcgctca gcattggggc
      781 agccatggtc gcctcctaga gggatttggc gagctggaag ctggcgatgg ccgcccaggt
      841 ccccagcccg aaaaggccca ggccgccgac cagcagcagg gcgttgatgg tcttcttgag
      901 tcctgccgaa ggcccgatct cacccacgat gttcctgaag ccgtacaggc cgtggaacag
      961 cgcggcgccg agcagcagga tgtagctgat ggtgaacaag gcgctcttgc ccctcgccac
     1021 cacgttcccc cagtccaccg ggtgcccccc tgccggattc ccgattccca cggtccggct
     1081 caagtgcatg acgaccatgt ggaggcccag gagcaccacg acgagcacgc ccgccgccac
     1141 atgccaggtc cacagcttct ggtcgcgcat ggattcgacc tcctacttca ggatgaagaa
     1201 gtccaggccc ccgatgacga cggccaccgc cgccagcacc atgaccccca cggccagcgg
     1261 ccgctgcacg tgtaccgagg tgcggtaggg atagaccggc tcgatgggtt tgcccactcc
     1321 cacgcccagt tccaagagca cgaggcggat tccgttgagg ccgtggatca cgaaggccgc
     1381 gaacaccagg tactcgccca gggtgaatat ggggccctcc accgtcttca tcgccctggc
     1441 ccaggcctcc ggccccagag tgcgggaggt ggtcaggacg atgtgcagca agaaataagc
     1501 gaggagcccc aggccggtga gacggtgcag gctgtacagg taccgctcga agccccagcg
     1561 gccgccaccc agccagcccc agacgcccag ccgattcggg tagtgcttgg tcgtgaacat
     1621 gagaccgtct cctcccgttt cctccgaccg tgatcgcgcc aggaccttcc cctcgttcct
     1681 accgcccctc ttcccatgcg aaggcctgcg gccattgaat tgggtctcag tactttcgtt
     1741 ctaccggctt ccaggtgttg atagtcaccg gcttggagct cagcttcggc ccagcctcgt
     1801 cgcggaacgc cagggtatgc ttgagccatt cctggtcgtc ccgggtcggg aagtcgcgcc
     1861 gggcatgggc accccgcgat tctttccgct ccagtgcccc ggccacggtc acctcggcca
     1921 ggtcgatcag gttctccagt tccagggcgt tgaacaggtt tgagttgtat accttgctct
     1981 tgtcgtccac cggcgctcgc ctgaagcgct cgcgaatctc cccgatcctc ttgagcgcct
     2041 cggtcagctc cacgcccttc cggtagacgc caacgtactt gtccatggtg cttcgaagat
     2101 cctgcttcac ctggtagagg ttctcgttgc cggtgcggtt gacaatctcc tccacgtgcc
     2161 ggcgcccctc ccgcacccgg gcctcggacg tggatgccac cttgagtctc gggagcgcct
     2221 tgacgatctc ctcgccgcag atcccgcccc acaccagaca ctcggcggtg gaattggaac
     2281 ccaaccggtt gccgccgtgc agggacacgc aggcggcctc ccctgccacc cagacgttcg
     2341 gaaggcgcgt gcgtccgttg ctgtcgcagt ccactccgcc cctggagtag tgcgccaccg
     2401 ggcggacggg gataggtttc tcaatcgggt cgagcccgat gaacttgatg cacacctcac
     2461 ggatcagggg caggcgggtg ttgatcttct cagcacccag gtgggtgaga tccaggtgga
     2521 cgtagtccag gccatccggg tgcgggaagc ctcgtccctc caggatctcg gtcatctcgg
     2581 cccgcgagac catgtcgcgt ggcgccagct ccatcttgga ggcggcgtat ttctccatga
     2641 agcgctcgcc cttggagttg cgaaggtagc cgccctcgcc gcggcaggcc tcggtcatca
     2701 ggatgcctga gggcaccaag ccggtggggt ggaattgcag gaattccata tcctcaaggg
     2761 gtagacctgc ccggtaggcc atggccattc cgtctccgga gacggtctgg gagtaggtag
     2821 tgaacccata caggctgcca cctccgccgg tggcgatgag caaggctttc ccttggagca
     2881 atacggtctc gccgctcgcg gcgtgaatcc ccagcaaccc ggcgaagcgg ccgtcctcca
     2941 ccaggatatc cgtgacgaag aattcatcgt accgcgtgaa
//
LOCUS       DBNGZC010000661         1747 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1129216, whole genome shotgun sequence.
ACCESSION   DBNGZC010000661 DBNGZC010000000
VERSION     DBNGZC010000661.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1129216"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..1569
                     /locus_tag="AC16G0_11505"
     CDS             46..1569
                     /locus_tag="AC16G0_11505"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HIR5100272.1"
                     /translation="MLASWVVDEMKDADLRDKRLNDRLQRILSDLSERPTASIPAACG
                     GKAETEAAYRFFNNEKALPQRILDPHYEQTRQRIAAQEVAILVPDTTELDLTRPHSQV
                     EGAGPLDDGPRRGALVHLLEAFSEDGTPLGAVWSQMWTRDEPADEPAADQPERNRKQI
                     PIEEKESYRWLESQRQARAVAQKVPGTKCVLTADSESDIYEVFAEPRGADNPLEWVIR
                     ACQDRAVKSGADDTAGQLWAGVSAEEVLFTREITVRARHAKVSCDQRKRRTERAARQA
                     TMEVRAARVELRPPWRPDRKLPAVFVNVVQVREIAPPEGEEPVEWILVTTLPIDTIED
                     VIAIIRYYTVRWMIEIFFRVLKSGCRVEQRRFEHLDRALTFVAVSLIVAWRVLMICRL
                     GRSCPDLDCEAVFEPSEWKAVYVAVHQRPAPEQPPPLGEMIRLIAQLGGYVNTPGRKD
                     PPGPQTLWLGMQRMSDLAWAWNTFGPVARAEAVSESVPAKPGRVRHPKAPGTRAKDV"
BASE COUNT          337 a          501 c          614 g          295 t
ORIGIN      
        1 tgacccgcac cacggacggt tttcatcagc caacggagtg gcatcatgtt agcatcctgg
       61 gttgtggacg agatgaagga cgccgacctc cgagacaagc ggctcaacga ccggctgcag
      121 cggatcctct cggacctctc tgagcgccct accgccagca tcccggcggc ctgcggcggc
      181 aaggccgaga cggaggctgc ttaccgcttc ttcaataacg agaaggccct cccccagagg
      241 atcctcgatc ctcattatga acaaacgagg caacggatcg ctgcccaaga ggtcgcgatc
      301 cttgtcccag acaccaccga actggatctc acccgcccgc acagtcaggt ggaaggggcc
      361 gggccgctgg acgacggccc ccgacgcggg gcactggtgc atctgctgga ggccttcagc
      421 gaggatggga cccctctggg ggcggtatgg tcgcagatgt ggacgcggga cgaaccggct
      481 gacgagcccg ccgcggacca gccagagcgc aatcgcaagc aaatcccaat cgaagagaaa
      541 gagagctatc gctggctgga aagccaacgg caagcccgcg ccgtggccca aaaggtgccc
      601 gggacgaagt gcgtgctcac ggccgacagc gagtcggaca tctatgaggt ctttgccgag
      661 ccgcgcgggg cggacaaccc gctcgagtgg gttatccgag cctgtcagga tcgcgcggtg
      721 aagtcggggg ccgacgacac ggccggtcaa ctctgggccg gtgtgtcggc agaggaggtg
      781 ctcttcaccc gcgagatcac cgtgcgggcg cggcacgcca aggtctcgtg cgatcagcgc
      841 aagcggcgga cggagcgggc ggcccgccag gcgacgatgg aggtgcgtgc cgcgcgggtg
      901 gagttgcgac cgccctggcg tccggaccgg aaactgcctg ccgtgttcgt gaatgtggtg
      961 caggtccgcg agatcgcgcc tcccgagggg gaggagcccg tggagtggat cctggtgacc
     1021 acgctcccca tcgacacgat agaagatgta atcgcaatca taagatatta tactgtgcgg
     1081 tggatgatcg agatcttttt ccgggtgctc aagtcgggct gccgggtgga acagcggcgg
     1141 ttcgagcacc tggatcgcgc gctgaccttc gtggcggttt cgttgatcgt ggcctggcga
     1201 gtgttgatga tctgccggct gggtcggagt tgcccggact tggactgcga ggcggtcttc
     1261 gagccgtcgg aatggaaggc ggtgtacgtg gcagtgcatc agcgtccggc cccggagcaa
     1321 ccgccgccgc tgggggagat gatccggctg attgctcaac tgggcggtta cgtcaacacg
     1381 cctggccgca aggatcctcc gggcccgcag acgctctggt tgggaatgca gcggatgtcc
     1441 gacctggcgt gggcgtggaa cacgtttggt cccgtggcga gggccgaagc ggtttcggag
     1501 tcggtgccgg ccaagccagg tcgggtcagg catcccaagg ctcctgggac aagggccaaa
     1561 gatgtgtagt acaacgaggg gggagcacat ggggcaagat ttctggaaat cgcgatgaaa
     1621 aaccaatttg tggcgcattc tgaaaatatg gcagttaccg catttaccgt tgtttactgg
     1681 gggacgttca tgatcgacac gattgcagag gagcgcgggc agggtcttcc gaaagatcct
     1741 ctggagg
//
LOCUS       DBNGZC010000662         2279 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1819571, whole genome shotgun sequence.
ACCESSION   DBNGZC010000662 DBNGZC010000000
VERSION     DBNGZC010000662.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1819571"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            558..1292
                     /locus_tag="AC16G0_11510"
     CDS             558..1292
                     /locus_tag="AC16G0_11510"
                     /inference="COORDINATES: protein motif:HMM:NF024257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR5100273.1"
                     /translation="MTWIGVLALMSVRTSASAEEVRLHGAASVADQLVNPYKAAVEQA
                     TGYTLAVVVSNAGRGLISLVEGKCDAAMASAALETTVKAAKAAGKEIDIATLQMHVAA
                     TDEVVFIVHPSNPVTSLTWEQIRDIHTGRITNWKAVGGKDLPITVFTDAPASATRGLI
                     KDSVLGGQEYSPQAQALDAVKSVNDQVATTEAGIGGLGKGFVDAAAVRVVQTRKLERP
                     LGFITLGNPSEKVQKVIEAYRSQARK"
     gene            1768..>2279
                     /locus_tag="AC16G0_11515"
     CDS             1768..>2279
                     /locus_tag="AC16G0_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5100274.1"
                     /translation="MRRRMGRWVQGMLLLLLASALVAVSLAGRADAQAPRTLVIAIGA
                     DQTGMDPQTVQNNESGFVMATIYDSIVNYKPGSSEVGPGLAESWTISSDGKIYTFKLR
                     RGVKFHDGTAMNARTVAEDLDRAINPNNPCYVLNRKVDTYDDFTFGSVKDGNVVKMDV
                     PDDYTLRFTLP"
BASE COUNT          438 a          706 c          750 g          385 t
ORIGIN      
        1 gggccgcggt gccgaagcgg tcggcgtacg ctcgctgatc atcaacatga gccagacggg
       61 gatgccgttc ctgtttggcg cgcttggaac ggcactcggc atgaccccgg tattctgggc
      121 catgtcggtg tgcctggtcg gcagcggctg catcgcgcgc aggcggtagc gccgcgggta
      181 cccgacggaa aggggagggc cgcttcccct cgttggactg tcctccggac tgggcgatct
      241 cgggcggacc gtcgcctatc gtttgcagca cacaatacgc ctggaccgtt gctcgtgact
      301 catcggcagt cctgtcatct tccgccgccc cctcagaacc gagcccgcat cccggcgatg
      361 attctgcttt tgaagtataa tgcagttggt gcatatatgg actacccgtg gcttgcctcg
      421 ggtagttccg gacgcgggca gggccacgac cccgcatgcc cctataggaa cgatcggaat
      481 gggggcctca tgcttgtcag agaatttgga tcgcagtcac aggaagacag gggggtcgga
      541 tgcgaatgac cgaggccatg acttggattg gcgtccttgc gctgatgtcc gttaggacga
      601 gcgcctccgc ggaagaagtg cgactccacg gagcggccag tgttgccgac caactggtca
      661 acccatacaa agcggccgtg gagcaggcca ccggctacac gctggccgtg gtggtcagca
      721 atgcggggag gggattaatc agcttggtcg agggcaagtg cgacgcggcg atggcctcgg
      781 ccgcgctgga gaccacggtg aaggcggcga aggccgcggg gaaagagatc gacatcgcca
      841 ctctccagat gcacgtggcg gccacggatg aggtggtctt catcgtccac ccctccaatc
      901 ccgtcaccag cctcacctgg gagcagatcc gagacatcca cacgggcagg atcacgaact
      961 ggaaagcggt gggcggcaag gacctgccca tcacggtctt cacggacgct ccagccagcg
     1021 cgacccgcgg actgatcaag gattcggtcc tcgggggaca ggaatattcc ccgcaggcgc
     1081 aggctttgga tgcggtcaag agcgtgaatg accaggtagc gacaaccgag gcgggcatcg
     1141 gcgggttggg caagggcttc gtggacgcgg ctgcggtcag ggtcgtgcag actcggaagc
     1201 tggagcgccc tctgggattc attaccctcg gaaatccatc cgagaaagtg caaaaggtga
     1261 tcgaggccta caggtcccag gcaagaaagt aagcgggcga tcggggtcgc gtgacatgca
     1321 gtttccgtgc attggtcatt ccccgccccc tctgcctgcc ggccagccgg gatccccacg
     1381 cgcgacgagg cgtgagccgc cgcaaaattc cccttgaccg cccccgccta ccgctgcatg
     1441 cttttttcgc tggcatttgg cggggcgacc cctccgcggg attccacgtt catcgccagc
     1501 cgtagggccg ccacaccgga gaagcaaaat ctgaccgcct tcacccataa tctgtgcacc
     1561 acattcgaag ggtcatgcgt ctgccacaac tcgtggtgcc gccgcgggtc tggcccagga
     1621 aggtgcggca actgccgcgc ctagaaccgc gattgacccc tgcgtatcac gaccgccgct
     1681 cgcatcgggg gggtgcgagc gtgtggtgaa cggacggagg cactgacgag gtcgtcgacg
     1741 acgtcctcgc acgaaaggag atcaggcatg agaagacgca tggggaggtg ggttcaaggc
     1801 atgctcctgc tcctgctggc aagcgcgctg gtggccgtca gcctcgccgg ccgcgccgac
     1861 gcgcaggcgc ccaggacact ggtgattgca ataggggcgg accagacggg gatggatccc
     1921 cagacggtcc agaacaacga gtccggcttc gtcatggcga cgatctacga ctcgatcgtc
     1981 aactacaagc ccggctcgag cgaggtgggg cccgggctgg cggagtcctg gaccatctcg
     2041 tccgatggca agatctacac cttcaagctc cgccgtggcg tcaagttcca cgacgggacc
     2101 gccatgaacg cccgcacggt ggcggaagac ctcgaccgcg cgatcaatcc gaacaaccct
     2161 tgctacgtgc tcaatcggaa ggtggacacc tatgacgact tcaccttcgg gtcggtgaag
     2221 gacggcaacg tcgtgaagat ggatgtgcca gacgactaca cgctccgctt cacgctgcc
//
LOCUS       DBNGZC010000663         6794 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1525322, whole genome shotgun sequence.
ACCESSION   DBNGZC010000663 DBNGZC010000000
VERSION     DBNGZC010000663.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6794)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6794)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6794
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1525322"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1183
                     /locus_tag="AC16G0_11520"
     CDS             191..1183
                     /locus_tag="AC16G0_11520"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR5100275.1"
                     /translation="MPVGGDPVQPPTLQDVYDARRVISRYLAPTPLVHSAAMSEVLGC
                     EVHLKLETATPIGAFKVRGGINLISQLSEEERGRGVITASTGNHGQSIAYAAKLFGVR
                     AVIGAPEGANPYKVAAMRRLGAEVILKGKDYDEAREWVEDQAKRKGYRYVHAANEPLI
                     IAGVGTASLEILESLPSVDAILVPIGGGSGISSHCVVAKQINSKVKIIGVQAEKAPAV
                     YLSWRNGRKVETPEAATWAEGLATRTPFDLTLDIIREHVDDIVLVSEEELRQGVLFLL
                     EAGHQLAEAAGAAPAAAAQKLKAQLRGQTVVLIVSGSNITLDQLRRVLTDPQPW"
     gene            1191..1655
                     /locus_tag="AC16G0_11525"
     CDS             1191..1655
                     /locus_tag="AC16G0_11525"
                     /inference="COORDINATES: protein motif:HMM:NF019301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR5100276.1"
                     /translation="MAENASRWSERLPGIALVLARLYTGQFFLQTGFTKLFRGFLWGG
                     VLLPQLERFAAGTPHLWYKAWLVNVVIPHEHLFAMLTSLGESLAGIALILGAFTRYAA
                     AGGIFMVANYLFSKGWPNPAASHDKDFIVILIVILIGGAGNYWGIDRWRRRR"
     gene            1669..2037
                     /gene="queF"
                     /locus_tag="AC16G0_11530"
     CDS             1669..2037
                     /gene="queF"
                     /locus_tag="AC16G0_11530"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HIR5100277.1"
                     /translation="MPTKPTRRLDTFPNPNASRDYEISFECPEFTCLCPRTGQPDFAT
                     IRIRYVPDRLCVELKSLKLYLWSFRDEGHYHEAMTNRILDDLVKATRPRRMTVVGDFY
                     VRGGIHTVVTATHERARRRR"
     gene            2130..2801
                     /locus_tag="AC16G0_11535"
     CDS             2130..2801
                     /locus_tag="AC16G0_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR5100278.1"
                     /translation="MRILLVEDDRKVASFIRKGLEEEGHAVDLAPDGETGLTMGLDRL
                     HDVIVLDVMLPGKPGLYVVRELRQAKVATPVLLLTARDAVEDKVQGLDAGADDYLTKP
                     FAFAELLARIRALLRRRTEAKGPLLQVADLALDPATRTVKRGAEPIALTNREFALLEY
                     LMRNAGRVLTRTMIAEHVWDYSFDSTTNVIDVYVNYLRKKVDAGHEPKLIHTVRGVGY
                     VLKET"
     gene            2888..4369
                     /locus_tag="AC16G0_11540"
     CDS             2888..4369
                     /locus_tag="AC16G0_11540"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5100279.1"
                     /translation="MKRLSIRARLTLWYTGIVAATLILLGGVAYGLLMRGLWQDVDAT
                     LESVAKTLAQTAHQPALEPLPSDLDEVIRRFFGPGMADKFYQFFDPRGRLDPRWPRFR
                     GEPLHVSPEALRNAARGDATFETVPEGERYPVRIITAPIMQNGQMVNILQVGMSLEGI
                     YLARRHFLWTLAALVPLALILAGTGGWLLARRALRPVDQMTTTARRIEAEHLAQRLEG
                     AEADDELGRLARTLNEMLARLEATFAQVRNFSSDASHELRTPLTVLKGEIEVALRNPR
                     EPGEYRRVLTSALEEVERMSRLVDDLLMLSRADAGALRWDHGSVELDRLVEEVAKQGD
                     ILGQGGGVRIRISTLEPLVVQGDDQRLKQLLLNLVGNAIKYTPSNGQVTLGLRAVKRE
                     AGGKRAEARDPEPSGFEPKSGGKDYAEITVQDTGIGIPAEALPRIFERFYRVDQARAR
                     EAGGAGLGLCIAKTIATAHGGQIEVQSTPGAGSTFTVILPLAS"
     gene            4674..>6794
                     /locus_tag="AC16G0_11545"
     CDS             4674..>6794
                     /locus_tag="AC16G0_11545"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR5100280.1"
                     /translation="MRCPQCQFENLDDARFCEECGTKLDQACLACGCAVSPGQKFCRN
                     CGQALTPAVAPSAKFTSPQAYTPKHLAEKILTSKSAMEGERKNVTVMFADVSGFTAMS
                     ERLDPEEVHTIMDRAFEVILDAVHRYEGTINQFLGDGVMALFGAPIAHEDHPHRALSA
                     ALAIQEDLKALREEVGRSHGVDFQVRMGINTGLVVVGAIGKDLRMDYTAVGDTTNLAA
                     RLMSLAQPGQVVVGRRTQHLREGFFVFEDLGEFQVKGKTNPIRAYALTGEIRGRTRLE
                     VSKERGLTPRVGRDSELAQLTDAYRRVERGEAAVVVVAGEPGVGKSRLLYEFLRGLTG
                     AGFLELEATCLGDARSTPYHAILQLVRSYLGLPFGMLPDEMQTRLAERLERLRIEAEE
                     DRTLIEHFLGISAPPEFLTRLGPQLKERTFAVFRELFLRASKTTPVILVVENLHWVDP
                     SSEDFLRHFISAVPGNRILLLLSTRSDYSAPWLTPPLAEMLALEGLKAENIYGMARAL
                     LDAQRVSEKLLNLLLAKGEGNPLYVEEILRQLQETRGIAVEEGEARLVGGEVSVPATI
                     HDIIAARIDRLPESVKRTLQASAVVGRRFTLPLLSRLVNEGLVDDLGKLQALDFVYLE
                     AQKPEVAYAFKHALTQEVAYTSLLERRRRLYHEAVGRGIEELHAGRLEEVAELLAHHF
                     GRSAEAEKAVDYAILAGEKAHRRWA"
BASE COUNT         1226 a         2134 c         2217 g         1217 t
ORIGIN      
        1 cgtgtcactg gggccagtgt gcgccggccg acactccacc ccgggaatca ttcgacgtgt
       61 cgagccagag cttatgcccg tggcgccccg gcgtcaaggc gcaactgcac gagaggtatg
      121 gaatcggttg ccgaccaacc cttgagcggt gtatgctttc gtggccaata tctatgtctt
      181 tcacgccagc atgcctgttg gaggagatcc cgtgcagcca cctacgctcc aggatgtgta
      241 tgacgcgcgc cgggtgataa gtaggtacct cgcgcccaca ccgttggtcc attcagccgc
      301 gatgtcagag gtattggggt gcgaggtgca cctcaagctg gagaccgcca cccccatcgg
      361 ggcattcaag gttcggggcg gaatcaatct gatttcccaa ctgagcgagg aggagcgtgg
      421 gcgcggggtc atcacggcat caacgggcaa ccacgggcaa tccatcgcct atgccgcgaa
      481 gctattcggc gtgcgggccg tcatcggcgc ccccgagggg gcgaacccgt ataaggtggc
      541 ggctatgcgc cgactgggcg cggaggttat cctaaagggc aaagactacg acgaggcaag
      601 agaatgggtg gaggaccagg cgaagcggaa gggataccgg tacgtccatg cagccaatga
      661 gccgctcatc atcgccgggg tcggcaccgc cagcctggag atcctggaat cacttccgtc
      721 cgtggatgcc atcctggtcc ccataggggg cggcagcggg atcagcagcc attgcgtcgt
      781 ggcgaagcag atcaattcga aggtcaagat catcggcgtc caggcggaga aggcgccggc
      841 ggtgtacctc tcctggcgga acgggcgaaa ggtggagacc cccgaggcgg cgacctgggc
      901 cgagggtctc gccacccgaa cccccttcga cctgaccctc gacataatcc gggagcatgt
      961 tgacgacatc gtcctggtca gcgaggagga gctgcggcaa ggggtcctgt tcctcctgga
     1021 ggccggccac caactcgccg aggccgcggg ggcggcgccg gccgctgcag cgcagaaact
     1081 gaaggctcaa cttcgaggac agacggtcgt cctgatcgtc agcggtagca acatcaccct
     1141 ggatcaactc cggcgggtcc tgacggaccc acaaccctgg taggtgtgcc atggcggaga
     1201 acgccagccg ttggtcggag cgcttgcctg gtatcgccct ggtcctggca agactgtaca
     1261 caggccagtt cttcctccag acgggcttca ccaagctctt ccgcggcttc ctctggggcg
     1321 gggtcctctt gccgcagctc gagcgctttg cggccggcac gccccacctg tggtacaagg
     1381 cctggctggt caacgtcgtg attccccacg agcacctctt cgccatgctt acctcgctgg
     1441 gtgaaagcct ggcaggcatc gccttgatcc tgggcgcctt cacccgctat gccgccgccg
     1501 gaggcatttt catggtcgcc aactatctct tcagcaaggg ctggccgaac cccgcggcat
     1561 ctcacgacaa ggatttcatc gtcattctga tcgtgatcct catcgggggt gccgggaatt
     1621 actggggaat cgacaggtgg cggcggcgcc gctgagagga gtccgtctgt gccgacaaaa
     1681 cccaccaggc gactcgatac ctttcccaac ccaaacgcca gccgcgacta cgagatcagc
     1741 ttcgagtgcc cggagttcac ctgcctttgc ccccgcacgg gacagccgga cttcgccacg
     1801 atccgcatcc gctacgttcc ggaccggctg tgcgtggagc tcaaatcgct caaactctac
     1861 ctgtggtcgt ttcgcgacga ggggcactat cacgaggcaa tgaccaaccg catcctggat
     1921 gacctggtga aggcgacccg gcctcgccgc atgacggtgg tcggggactt ctatgtgcgt
     1981 ggcgggattc acaccgtcgt gaccgccacc cacgagcggg cgagacggcg gcgctgaatg
     2041 gaaactcgac ggagtatggg aataggtctg gggtggttcc gggatgtctc ggtttcctgg
     2101 tggtgacaca atcggcctcg gtttctccca tgcgcatcct gctggttgag gacgatcgga
     2161 aggtcgccag tttcatccgc aaggggctcg aagaggaagg ccatgccgtg gacctcgccc
     2221 cggacggaga gactggcctg acgatgggac tggaccggct tcacgacgtc atcgttctcg
     2281 acgtcatgct tccggggaag ccgggccttt acgtggtgcg ggagctccgc caagccaagg
     2341 tggccacacc cgttctcctg ctcaccgcac gggatgcggt ggaagacaag gtgcaggggc
     2401 tggatgccgg ggcagacgat tatctcacca aacccttcgc ttttgccgag ttgctggccc
     2461 gcatccgggc cctgctgcgt cgccggacgg aggcgaaggg tccgctcctt caggttgccg
     2521 acctcgccct ggatccggcg acccgaacgg tgaagcgggg agcggaaccc attgcgttga
     2581 ccaaccgtga gtttgccctc ctggagtatc tcatgcgtaa cgcggggcgg gtcttgaccc
     2641 ggacaatgat tgccgagcac gtctgggatt actccttcga ctcgactacc aatgtcatag
     2701 acgtgtatgt gaactacctc cggaagaagg tcgacgcggg tcatgagccc aagctcatcc
     2761 acaccgtccg cggcgtgggt tatgtcttga aggagacctg acgacgaccg cagattcaat
     2821 ggatcagctt cctggtctga ttccaatctg tgcagcctgc gaaatccgag cctagcaggg
     2881 gtcctgcatg aaacggcttt ccatccgagc ccggttgact ctctggtaca cggggatcgt
     2941 ggccgccacc ctcatcctct tgggcggcgt ggcctacgga ctgctgatgc gtggcctgtg
     3001 gcaggatgtg gacgccacgc tggaaagcgt cgccaagacg ctcgcccaga cggctcatca
     3061 gccagccctg gagccgctcc cttcggacct cgacgaggtg attcgccgat tcttcggccc
     3121 cggtatggcg gacaagttct accagttctt tgatccccgc ggccgcctcg atccccgctg
     3181 gccccggttt cgtggggagc cgctgcacgt gagcccggag gccctccgca atgccgcgcg
     3241 aggggatgcc accttcgaga ccgtgccaga gggtgagcgc taccctgtgc gaatcatcac
     3301 cgcccccatc atgcagaacg gccagatggt gaacatcctc caggtgggaa tgtcgctgga
     3361 gggcatctac ctggcacgtc gacatttcct ctggacgctg gctgccctgg tccccctggc
     3421 gctgatcctg gccgggaccg gcggctggct cctcgcgcgg cgcgccctcc gccctgtgga
     3481 ccagatgact accacggccc gacggatcga ggcggagcac ctggcccaac gtctggaggg
     3541 cgcggaggcg gacgatgagc tgggacgact ggcccggacg ctgaacgaga tgttggcccg
     3601 gctggaggcg accttcgctc aggtccgcaa cttctcttcc gacgcctccc acgaactccg
     3661 gaccccgctg acggtgctca agggcgaaat cgaggtggcc ctgcgaaacc cgcgggaacc
     3721 gggagaatac cggcgcgtcc tgaccagcgc cctggaagaa gtcgagcgga tgtcccgcct
     3781 ggtggacgat ctcctcatgc tgtcgcgggc ggatgccggg gctttgcggt gggaccatgg
     3841 atcggtcgag ctggatcgtc tggtcgaaga ggtcgccaag cagggcgata tcctcggaca
     3901 gggaggcggg gtgcgaatca gaatcagcac cctggagcct ctggtcgtcc agggagacga
     3961 tcagcgcctg aagcagttgc tgctcaacct cgtgggcaac gccatcaagt acacaccctc
     4021 caacggccag gttacactgg gactgagggc tgtaaagcgg gaggcgggag gcaagagggc
     4081 ggaggcaaga gatcctgagc cgtcgggctt cgagccgaag tctggcggaa aggactatgc
     4141 ggagatcacc gttcaggaca ccgggatcgg catccccgcc gaggctcttc cccgaatctt
     4201 cgagcgcttc taccgcgtgg atcaggcgcg cgcgcgcgag gccggcggcg ccggcttggg
     4261 cctctgcatc gcgaagacca tcgccacggc ccatggcggc cagatcgagg tccagagcac
     4321 tcccggtgcc ggcagcacgt tcacagttat cctccccctg gcgagttagc ccggcctgcg
     4381 tattcagctc ccccagtctt ttttgccatc cgacgcgacc cctcctctgt agatttgtca
     4441 tgtctgggaa gaaagacgag acgggccttc gccttgacaa gcgagagagc aatgcatagg
     4501 ttttcagaag aacattccgc ctcaatcgtg cgctgagggg aggcacccct gcctgtcacg
     4561 gggtttcttc cgggaatgca ggaattcgac ccttcaggag tggggtgctc ggtaggatcg
     4621 caacatttgc caacgcgtga ggtccccaca caacccgaag attgaggacc cagatgcgtt
     4681 gcccgcagtg ccagtttgaa aatctggacg acgcccgctt ctgcgaggag tgcgggacga
     4741 agctggatca ggcctgtctc gcctgcggct gcgcggtttc ccccggccag aagttttgca
     4801 gaaactgcgg gcaggctctt acccccgcgg ttgctccgtc ggccaagttc acctcccccc
     4861 aggcctacac cccaaagcac ctcgccgaga agattctcac ctccaagagc gccatggagg
     4921 gggaacggaa gaatgtgacc gtgatgttcg cggacgtctc cggtttcacc gccatgtccg
     4981 agcgcctgga ccccgaggaa gtccacacga tcatggatcg cgccttcgag gtgatcctgg
     5041 acgccgtcca ccgctacgag gggacgatca atcagttcct gggggacggg gttatggcac
     5101 tgttcggggc cccgatcgcc cacgaagatc atccccatcg cgccctgagc gccgcgctgg
     5161 ccattcagga agacctgaag gcgctccggg aggaggtggg gcgctcccat ggggttgatt
     5221 tccaggtccg gatgggcatc aatacggggc tcgtggtggt gggcgccatc ggcaaggatc
     5281 tccgcatgga ctatacggcg gtgggggaca caaccaacct cgcggcgcgt cttatgagcc
     5341 ttgctcagcc gggtcaggtc gtcgtcggtc ggcgcaccca gcacctccga gaaggattct
     5401 tcgtcttcga ggacctgggc gaattccagg tgaagggaaa gaccaatccg attcgcgcgt
     5461 acgccctgac gggcgaaatc cggggccgaa cccgtttgga agtgtccaaa gagcgtgggc
     5521 tcaccccgcg ggtcggccgg gattccgagc tggcgcagct cactgatgcc taccggcggg
     5581 tcgagcgggg tgaggcggcc gttgtcgtgg tcgccggcga gccgggcgtg ggcaagtccc
     5641 gattgcttta cgagttcctg cggggactga ccggggccgg gttcctggag ctggaggcca
     5701 cctgcctggg ggatgctcgt tcaacacctt accatgcgat cctgcagctc gtccggagct
     5761 acctggggtt gccgttcggg atgctgcctg atgagatgca gacgcgcctg gctgaacggc
     5821 tggagcggct gcggatagag gcggaagaag accggacgct gattgaacac tttctcggga
     5881 tctcggctcc cccggaattc ctcacgcgtc tcggccccca gctcaaagag cgaacctttg
     5941 cggtgttccg tgaattgttc ttgcgggcca gcaagaccac cccagtcatc ctggtcgtgg
     6001 aaaacctgca ctgggtcgat cctagctcag aggatttcct ccgccatttc atttcagccg
     6061 tgcctgggaa tcgaattctc ctgctcctga gcacgcggtc tgactactca gccccgtggc
     6121 tcacgccgcc gctggccgag atgctcgcgc tcgaaggcct gaaggcggag aacatctacg
     6181 ggatggcccg cgccctgctc gacgcgcagc gggtctccga gaagctcttg aatctattgc
     6241 ttgccaaggg cgaagggaac ccactctacg tggaggagat cctccggcag ttgcaggaga
     6301 cgcgggggat cgccgtcgag gagggggagg ccaggctggt cggtggggag gtcagcgtcc
     6361 ccgcaaccat tcacgacatc atcgccgccc gtatcgaccg tctgccggag tcggtcaagc
     6421 gcacgttgca ggcctccgct gtggtgggcc ggcgatttac actccccttg ctgtcgcggc
     6481 tcgtcaacga ggggctggtg gatgatctgg gcaagctcca ggcgctcgac ttcgtctacc
     6541 tggaggccca aaaaccggag gtcgcctacg ccttcaagca cgcgctcacg caggaggtgg
     6601 cttacaccag cctgctggag cgccggcggc gcctgtacca tgaggccgtc ggccgcggca
     6661 tcgaggaact gcacgcgggg cgcctcgagg aggtggcaga gctgctggcc caccacttcg
     6721 ggcgaagtgc cgaggcggaa aaggccgtgg actacgccat cctcgccggt gagaaggccc
     6781 atcgtcgctg ggcc
//
LOCUS       DBNGZC010000664         1671 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_546310, whole genome shotgun sequence.
ACCESSION   DBNGZC010000664 DBNGZC010000000
VERSION     DBNGZC010000664.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_546310"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="AC16G0_11550"
     CDS             <1..767
                     /locus_tag="AC16G0_11550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MsnO8 family LLM class oxidoreductase"
                     /protein_id="HIR5100281.1"
                     /translation="VAENFRMLETLFPGRIDLGIGRAPGSDQRTARALANDLGALDAE
                     RFPGKVTDLIGFLHNDLGADHPLTGIEAMPNGSGTPEVWLLGSSDQSAGLAARVGTAF
                     SFAHFISSEGASEILRAYADQFRPSPYLARPQASLAVFVVCADTEAEALRLARSRDLF
                     LLWLRAGRSLPYPSVGEAEGYSYSARERVLVEHNRRRIIAGAPEQVRDRLLTMGEACG
                     VQEFVVVTITHDFKARLRSYELLAEAFGLDRQETAG"
     gene            870..1109
                     /locus_tag="AC16G0_11555"
     CDS             870..1109
                     /locus_tag="AC16G0_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100282.1"
                     /translation="MKLRSALMHGLTWSARLSSLPADSPGGQAVLVLDNEEILASEDA
                     AFGEFSIVEASAEEREALRQAGYSLPDWTPEEQPV"
     gene            complement(1161..1568)
                     /locus_tag="AC16G0_11560"
     CDS             complement(1161..1568)
                     /locus_tag="AC16G0_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HIR5100283.1"
                     /translation="MARPDNRLQVIKTAKAGPPVGPYSQGIKAGGFIFVAGEKGIDPL
                     TGKIVPGGIEAETRRTLENIKAILEEAGSSMDLAVSTSVFMTDLGDFHKMNAIYAEYF
                     RANPPGRTTVEVKALPAGAHVEITVTALAGSGS"
BASE COUNT          278 a          545 c          533 g          315 t
ORIGIN      
        1 aggtggcgga gaacttccgc atgctggaaa ccctcttccc tggtcggatc gacctcggca
       61 tcggccgggc gccgggcagc gaccagcgca cggctcgcgc cttggccaac gacctcgggg
      121 cactcgacgc cgagcgattc cccgggaagg tgactgacct gatcggtttc ctacataacg
      181 acctcggagc cgaccatcca ctcaccggta tcgaggccat gcccaacgga tcggggacgc
      241 cggaggtgtg gctcctcggg tccagcgatc agagcgccgg cctggcagct cgcgtgggga
      301 ccgccttctc cttcgcccac ttcattagca gcgagggcgc cagcgagatc ctccgcgcct
      361 atgcagacca attccggccg tcgccctacc tggcccggcc gcaggcgagc ctggctgtct
      421 tcgtcgtctg tgccgatacg gaggcggagg ccctgcgtct ggcgcgaagc cgtgatctct
      481 ttctcctctg gctgcgcgcc ggccgatccc tcccctaccc atcggtcggg gaggcggaag
      541 gctattcgta ttcggctcgc gagcgggtcc tggtggagca caatcgccgg cgtatcatcg
      601 ccggcgcccc ggagcaagtc cgggaccgtc tgttgacgat gggggaagcc tgcggtgtgc
      661 aggagttcgt ggtcgtgacc atcacccacg acttcaaggc gcggctgcgc tcgtacgaac
      721 tcctggccga ggccttcggg ctggacagac aggagaccgc tggatgaacg tcgacagttc
      781 gactgattcg aagcgcgagg gctgaagggg agacggccca aggtcagtca aggagcagcg
      841 gcagtttgcc agggaggaaa gaaagatcca tgaagcttcg ctccgcactc atgcacggct
      901 tgacgtggag tgccaggctc tctagcctgc ctgccgattc gcccggcggg caagctgtcc
      961 tcgtcttgga caacgaggag atcctcgctt cagaggatgc tgccttcggc gaattcagca
     1021 tcgtcgaggc ctccgccgag gagcgggagg ccctgcgtca ggccggctac tcccttccgg
     1081 actggacgcc cgaggagcag ccggtttgag cctggcatcc tgaacctgga atcgagccgg
     1141 cgagttgatg tctggagaga tcaggaccct gagccggcca gggccgtgac ggtgatctcc
     1201 acatgggccc ctgcgggcaa tgccttgacc tcgacggtgg tgcgaccagg cgggttcgcg
     1261 cggaaatact ccgcatagat cgcgttcatc ttgtgaaagt caccgagatc cgtcatgaac
     1321 accgaggtgc tcacggcgag atccatgcta gacccggcct cctccaggat ggccttgata
     1381 ttctccaggg ttcgcctggt ctccgcctcg atccccccgg gaacgatctt ccccgtcaac
     1441 ggatcgatcc ctttttcgcc ggccacgaag atgaacccgc ccgccttgat tccctgagag
     1501 taggggccca ccgggggccc ggccttggcg gtcttgatga cctgaaggcg attgtcgggt
     1561 ctggccattc gcttgcctcc tttcgggtta ttcagtcctg cgtccccatt ccttagtgtc
     1621 ctgtccctga agttgaccac ataattgagg ccgttccggc tcgccctgag c
//
LOCUS       DBNGZC010000665         2241 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1621452, whole genome shotgun sequence.
ACCESSION   DBNGZC010000665 DBNGZC010000000
VERSION     DBNGZC010000665.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1621452"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="AC16G0_11565"
     CDS             <1..959
                     /locus_tag="AC16G0_11565"
                     /inference="COORDINATES: protein motif:HMM:NF014476.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIR5100284.1"
                     /translation="YLSRADVERVGLPMGEIIAAVEEVFREKGEGRVEMPAKIGIHPM
                     ADAYLRAAPAYLQSPPIAGVKWVAMFNQNPAKGLQTISGLLILNDPGTGHPLAVMDCT
                     WITAKRTGAASAVAAKYLARKDAAHLTIIGCGVQGRSHLEAMLVQFPGIRRIVAYDQR
                     PATLARYVREMQATHGVPLEAATDCESAVRDADIIVTATEILRQPKPVIRPELIKAGA
                     FCMPIDFDAQFTLEAIRAMDLFYTDDTPQMEYYQSLGYIAGVPRAQGDLGLLVTGRKP
                     WRTSTTQRTMSMQLGLAIEDMVTAMRIYERARAECIGTRLPL"
     gene            1065..2231
                     /locus_tag="AC16G0_11570"
     CDS             1065..2231
                     /locus_tag="AC16G0_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5100285.1"
                     /translation="MSSFKGVDYYEIEGLFSEEERMVRDVARAFTDEQILPIITPHFR
                     EGTFPVDLVRKLGALGFLGANLQGHGCAGIGNVAYGLIMQELERGDSGVRSFASVQGS
                     LVMYPIHAFGSEAQKEYWIPRLAAGEAVGCFGLTEPDFGSDPGNMATKAVRVGSEYVL
                     NGTKLWITNGSVADIAVVWAKLDGVVRGYLVEKGRPGFTSRDLHGKFSMRASVTSELA
                     FQECRIPADNLLPKSDGLRSPLSCLNQARYGIAWGAVGASMACYQAALDYSRGRIQFG
                     KPIASFQLVQQKLVHMLTEITKAQLLCLQLGRLKDAGRLRHTQVSLAKRNNVAVALRI
                     AREAREILGANGIVDEYPVIRHLLNLESVYTYEGTHDIHTLIVGRDITGISALE"
BASE COUNT          407 a          699 c          753 g          382 t
ORIGIN      
        1 tctacctgtc gcgggcggat gtggagcggg tcggcctccc catgggagag atcatcgccg
       61 ccgtcgagga ggtgtttagg gagaaggggg aaggccgggt tgaaatgccg gccaagatcg
      121 gcattcatcc gatggccgac gcctacctgc gcgcggcgcc ggcctatctc caatcccccc
      181 ctatcgccgg tgtcaagtgg gttgccatgt tcaaccagaa tccggccaag ggccttcaga
      241 cgatcagcgg cctgctgatc ctgaatgatc ctggaacggg ccatcccctc gcagtcatgg
      301 actgcacctg gataaccgcc aagcgcacag gggcggccag cgccgtggcg gccaagtacc
      361 tggcccgaaa ggatgccgcg cacttgacga tcatcggctg cggggtgcaa gggcggagcc
      421 acctggaggc catgctggtc cagttcccgg gtattcgccg gatcgtggcc tacgatcaac
      481 ggccggcgac gctcgcgcgc tacgtccggg agatgcaggc gacgcacggc gtccctctgg
      541 aggctgcgac cgactgcgag tcggccgtgc gggacgcaga catcatcgtg accgccacgg
      601 agatcctgag gcagcccaag ccggtcatcc gtccggagtt gatcaaggct ggggcgttct
      661 gcatgcccat cgacttcgac gcgcagttca cgctggaggc gatccgggct atggatctct
      721 tctacacgga tgacacgccc cagatggagt actatcagag cctcgggtac attgccgggg
      781 tcccgagggc tcaaggcgat ctcggcctac tcgtgacggg gaggaagccg tggcgaacca
      841 gcacgacaca gcggacgatg agcatgcagc tcggcctggc gatcgaggac atggtgacgg
      901 ctatgcgaat ctacgaacga gcccgcgcgg agtgcatcgg gacgcgacta ccgctgtgac
      961 gggggaccgc cttggaccag ccgcctttct cgggcagttc cgagggctcc acactggcgg
     1021 ctgaccactg gccgtttcct tcactggctg aaagggcagg gaacatgagc agcttcaaag
     1081 gtgtggacta ctacgagatc gagggactct tctccgaaga agagcggatg gtccgcgacg
     1141 tggcgcgtgc cttcaccgac gaacagatcc tgccaatcat cacgccccac ttccgggagg
     1201 gaacgttccc cgtcgacctg gtccggaagc tgggggcgct gggattcctg ggggccaacc
     1261 tccaggggca cggctgtgcc ggaatcggca atgtcgccta cggcctgatc atgcaggagt
     1321 tggagcgtgg cgattcgggc gtgcgaagct ttgcgtccgt ccaggggtcg ctggtcatgt
     1381 acccgatcca cgccttcggc tccgaggcac agaaggagta ctggattccg cgattggcgg
     1441 caggggaggc cgtcgggtgt ttcggtctga ccgagccaga cttcggctcg gatccaggca
     1501 acatggcgac caaggcggtg cgggtcggga gcgagtacgt cctgaacggc accaagctct
     1561 ggatcacgaa cgggagcgtc gccgatatcg ccgtggtctg ggccaagctg gacggcgtcg
     1621 ttcgggggta cctggtggag aagggccgcc caggcttcac ctcgcgcgac ctccacggga
     1681 agttttccat gcgggcctcg gttacctcgg agctggcctt ccaggagtgc cgaatccctg
     1741 ccgacaacct cctgccgaag agtgatggcc tgcgctcgcc gctgtcctgc ctgaaccagg
     1801 cgcgctacgg gatcgcctgg ggggcggtgg gggcctccat ggcgtgctac caggcggctc
     1861 tggactacag ccgggggcgc atccagttcg ggaagcccat cgcctccttc cagctcgtcc
     1921 agcagaagct cgtccacatg ctcacggaaa taaccaaggc acaattgctg tgccttcaac
     1981 tcggccgcct caaggatgcc ggccgcctgc gccacaccca ggtgtccctg gccaaacgga
     2041 acaacgtggc ggtcgccctg aggattgccc gggaggctcg ggagatcctg ggggccaacg
     2101 gcatcgtgga tgagtatccg gtgatccgcc atctcttgaa cctggaatcg gtctatacct
     2161 acgagggcac ccacgatatc cacaccctga tcgtgggacg ggacatcacg ggcatctcgg
     2221 ccctggagta agcccatggt t
//
LOCUS       DBNGZC010000666         2889 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1033639, whole genome shotgun sequence.
ACCESSION   DBNGZC010000666 DBNGZC010000000
VERSION     DBNGZC010000666.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1033639"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(297..1571)
                     /locus_tag="AC16G0_11575"
     CDS             complement(297..1571)
                     /locus_tag="AC16G0_11575"
                     /inference="COORDINATES: protein motif:HMM:NF033519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HIR5100286.1"
                     /translation="MAEAQSPTVHRCGCRECQRDPHGALASQHQAINTLIGCLDERSQ
                     RLIVGSLASQYGRGGVARLARITGLSRNTIRRGQRELARPASLSPGRIRRPGGGRKRV
                     EKKYPEILEALEELLRDATAGDPMTGLKWTHKSIRKLSAALHQRGLPAGHDAVARLLR
                     DQRFSLRTNRKLHAGTRDPDRDRQFRYLTRLRRWYLTRGLPVISVDTKKKELVGNFKN
                     PGRCWRRDSRAVLDHDFPSWAAGHGIPYGIYDVGRNAGYVVIGTSHETPAFAVAAIRR
                     WWIQVGRKHYSGSNRLLIEADGGGANGSRNAAWRAGLQALADEFGLIITVTHYPPGAS
                     KWNPIEPRMFSLISENWAGEPLANYETMLKFIRRTRSSTGFHCKACLDTTPYAKGVKV
                     TKQEQARLRLRRRPVMPKWNYTIWPRKAHKAK"
     gene            1702..2142
                     /locus_tag="AC16G0_11580"
     CDS             1702..2142
                     /locus_tag="AC16G0_11580"
                     /inference="COORDINATES: protein motif:HMM:NF020859.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HIR5100287.1"
                     /translation="MPDSLERLDLLLLTVPTARRVHQDGIRFMGLRYIDPTLAAYVGE
                     DVVLRYDPHDMAEIRVFYHDRFLCRAICQELAGATVTLREIVNARNARRRQLRQTLQE
                     RRRLVDSLLAAKRSPPVKQEQESSSPSSEPTPPPVKLKRYFCDE"
     gene            2135..>2889
                     /locus_tag="AC16G0_11585"
     CDS             2135..>2889
                     /locus_tag="AC16G0_11585"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5100288.1"
                     /translation="MSSGAAAMFIETQEYSRFREFCDACRTYQYIGLCYGFPGVGKTL
                     SARYYTDPKKLLPPFYWPSETNFEKGLGNHVVLYTPPVANSPGRVASDLSRWRHCVSM
                     TLASHFRSEETWKLRELEESMEVNWFAAYRNGGVDADTDEAAEYSRARDAYYAEWKRY
                     HSRYQEIQSVPVLVVIDEVYRLKMASLEQVREIFDEGGMGLVLIGMPGLEKRLARYAQ
                     FYSRVGFVHEFRSLAEKEVRGLLSQGWMPPGVV"
BASE COUNT          522 a          946 c          881 g          540 t
ORIGIN      
        1 ccccaccgcc attgctcggt tccacatgga cagaagtgta ttatgaactg gtggacaggt
       61 caaggaagaa agctgaggaa gcgcaccgct gccctactcc ctcgccggcc atggccgacc
      121 cggcaacatc ccgaccgccg cagcctgatc aaacgcccgc tgccacccct cggagtccgc
      181 ctgggccagt tcacgagcgg cacacccggc atcgggaccc gaggaagcaa ctggccgatc
      241 ccctcggccc ggccgcgctg ccgagtcaga taagtcggcg cccggaaaca agttgatcac
      301 ttggccttgt gagcttttcg tggccagatc gtgtaattcc atttgggcat gaccggacga
      361 cgtctcaagc gcagccgcgc ctgctcctgt tttgtcacct tcaccccctt ggcataggga
      421 gtcgtatcca agcacgcttt gcagtgaaag cccgtactcg accgcgtcct gcggatgaac
      481 ttcaacatcg tctcatagtt ggccagcggc tccccggccc agttctcgct gatcaggctg
      541 aacatccggg gctcgatcgg attccatttc gatgccccgg gcggatagtg agtgactgtg
      601 atgatcaggc cgaactcatc ggccaaagct tgcaggcccg ctcgccacgc cgcattccgg
      661 ctcccattgg ctccgccgcc atccgcctcg atcagcaggc ggttgctccc cgagtagtgc
      721 ttgcgcccga cctggatcca ccaccggcgg atcgcagcga cggcgaaggc cggtgtctca
      781 tgcgacgtgc cgatgacgac atatcccgca ttccggccca catcgtagat gccatacggg
      841 atgccatgcc cggccgccca actggggaag tcatggtcca gcaccgcccg gctgtcccgc
      901 cgccagcacc gtcccgggtt cttgaagttc cccaccagtt ccttcttctt cgtgtccacg
      961 ctgatgacgg gcaaccctcg ggtcagatac caccgccgca ggcgcgtcag gtaacggaac
     1021 tgccgatccc gatccgggtc ccgcgtcccg gcgtggagct tgcggttggt ccgcaaggag
     1081 aatcgctggt cccgcagcag gcgggccaca gcgtcatgcc cggcgggcaa cccccgctgg
     1141 tgcagagccg ccgacagctt gcggatcgat ttgtgggtcc acttcagtcc cgtcatgggg
     1201 tctccggcag tggcatcacg caagagttcc tccaacgcct ccaggatctc gggatatttt
     1261 ttttcgacac gtttgcgtcc gcctcccggt ctccggatgc ggcccgggga tagggaagcg
     1321 gggcgggcca gttctcgttg cccgcgccgg atggtgttgc gactcaaacc cgtgatccgg
     1381 gccaggcggg caacacctcc acgaccgtat tggctggcca gggaccccac gatcagtcgc
     1441 tggctccgct catccaagca gccgatcagg gtgttgatgg cctggtgttg ggaggcgagg
     1501 gccccgtgag ggtctcgttg gcattcccgg caaccgcagc ggtgaaccgt gggactttgt
     1561 gcctcagcca tgggccgcct ccgtcacggg tggcgatacc gtgacgaagg ctatgataca
     1621 gcggaatggt caagttatta ccgggcgccc cctaagcagg cgccccaggc ccgctgggat
     1681 gctggcggct tcctgccgca aatgccggac tcgctcgaac gactcgatct gctcttgctc
     1741 accgtgccca cggcgcgccg cgtccatcag gacggtattc ggttcatggg gctgcgctat
     1801 atcgatccaa cgcttgccgc gtatgtggga gaggatgtcg tactgcgcta cgacccgcac
     1861 gacatggctg agatccgcgt cttctatcac gatcgctttc tctgccgcgc catctgccag
     1921 gaactggcgg gcgcgaccgt gaccttgcgc gagatcgtta acgcccggaa cgcccgtcgt
     1981 cggcagttgc gccagacgct gcaggagcgt cgccgactcg ttgactcact gctggcggcc
     2041 aaacgctcac cccctgtcaa gcaagagcaa gagtcatcgt cgccatcgtc cgagcccacc
     2101 ccaccgccgg tcaagctcaa gcggtatttc tgtgatgagt agcggcgcgg cggcaatgtt
     2161 catcgaaacc caggaataca gccgctttcg agaattctgc gatgcctgcc gtacctacca
     2221 atacatcggc ttatgctatg ggttcccggg cgtcggcaag acactctccg cgcgttatta
     2281 caccgacccc aagaagcttc tgcccccgtt ctattggcct tcggaaacga acttcgagaa
     2341 ggggctggga aaccatgtcg tcctctacac gccccctgtg gcgaactccc caggaagagt
     2401 cgcgtcggac ctcagcaggt ggcgccattg cgtcagcatg accttggcga gtcatttccg
     2461 gagtgaagaa acgtggaagc tcagggaact cgaggagagc atggaggtca actggttcgc
     2521 ggcgtaccgc aacggtggcg tcgacgccga caccgacgag gccgcagaat actcgcgcgc
     2581 gagggacgct tactacgcag aatggaagcg ataccactcc cgctaccaag aaattcagag
     2641 cgtacccgtg ctcgtggtga ttgacgaggt gtaccgcttg aagatggcga gcctggagca
     2701 ggtccgcgag attttcgacg agggaggcat gggcctggtg ctgatcggga tgccggggct
     2761 cgagaaacgg ctcgcgcgct acgcgcagtt ttactcgcgc gtcggcttcg tccacgaatt
     2821 ccgatccctc gcggagaagg aagtgcgcgg cctgctcagc caggggtgga tgcctccggg
     2881 ggtggtgtt
//
LOCUS       DBNGZC010000667         1995 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_546422, whole genome shotgun sequence.
ACCESSION   DBNGZC010000667 DBNGZC010000000
VERSION     DBNGZC010000667.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_546422"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..620)
                     /locus_tag="AC16G0_11590"
     CDS             complement(201..620)
                     /locus_tag="AC16G0_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100289.1"
                     /translation="MRLLAVLQALTCFALLAGRGRFRTTDLHATAAEAQGKTPASYSL
                     GQLRYDLAKLRGKGLVERVAGTQSYRLPAEGYRLAVLYLKLFHRIYAPLTAGIVDPVP
                     WDDRLPPERRATLDRLYAAVDRALNQLFESVGVRLAG"
     gene            complement(622..1818)
                     /locus_tag="AC16G0_11595"
     CDS             complement(622..1818)
                     /locus_tag="AC16G0_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100290.1"
                     /translation="MNAFIEHHQQAIRFDYSCFDRILLNGVIQVLQNPACLVGFLKEK
                     RQATQVTPAYFKAISTDYHHFVQALADQRHVEIVQPPKGVRREEWVEPYYQKLQGQNA
                     IAVILKSRENARVAVSFPRQGNHVELCNRFVQQYYFYLQDRDFGRMFLRVCPYFPFSA
                     RVCLNGHEWLACRLREEGIAFEQCANAFRTCADPTRLQELADQFSPAHIEACAHRWLA
                     QLVPFFTDRERRHQGFGYRLFVSQVEYCTNLVFEHRAALDRLHDRLLDLNRSIGHPDK
                     IAMIFGRRITTHTDAGLKTQILDHDLGQPVIRSEYKSSSIKQYARQSGAADRDDQLSH
                     ARPGGEQGRGAPAGVAADDGRGQRPLSRRAAGRPGDLRRSRPIGAVATTDRLAERATD
                     PGSEAR"
BASE COUNT          330 a          598 c          689 g          378 t
ORIGIN      
        1 tagagatgat gggctcagcg aatcacggcg tcgtcgcccg tcggcgtctg agattcgtgg
       61 tgggacggtt gacgtcgcaa ggcgcgagct cccgcggttc cagccgcctc aacccttctc
      121 ttaggccgtt ataagacctg aagtgcgagt tctgttcacg tttttccaac tcatttattc
      181 tctgtgagtt gcgactcctt tcagcccgcc agcctgactc cgacggactc gaacagttga
      241 ttcaaagctc gatccaccgc agcgtacaac cgatccagcg tcgctcgacg ctccggcggt
      301 agccggtcgt cccacggcac cgggtccacg atcccggctg tcagtggcgc atagatccgg
      361 tggaacaact tcaggtacag caccgcgagc cggtacccct ccgccgggag gcggtaggac
      421 tgggtgccgg cgacccgctc caccagcccc ttgccacgca gtttggccag gtcgtaccgc
      481 agttgtccca ggctgtagct cgccggcgtt ttcccctgcg cctccgcggc cgtggcatgc
      541 aggtcggtcg tccggaaccg gccccggccc gccagcagag cgaagcaggt cagcgcctgg
      601 agcaccgcca gcagacgcac gtcatcgagc ttcagacccg gggtccgtcg cccgctcggc
      661 gagacggtcg gttgtcgcaa ccgctccaat tggccgcgat cgacgaaggt ctccaggacg
      721 tcctgctgca cgtcgagata gcggtcgttg gccgcgacca tcgtctgccg caactccggc
      781 aggtgctcca cgcccttgtt cacccccagg tcgggcgtgt gatagctggt cgtctcggtc
      841 cgcagcacca gattggcgcg catattgctt gattgaactg ctcttgtact cgctccggat
      901 gaccggctgc cccaggtcgt ggtcgaggat ctgggtcttc aggcccgcgt cggtgtgcgt
      961 cgtgatccgt cggccgaaga tcatcgcgat cttgtccggg tggccgattg agcggttgag
     1021 gtcgaggagg cggtcgtgca ggcggtccag cgccgcgcgg tgctcgaaga ccaggttggt
     1081 gcagtactcg acctgactga cgaacaggcg atagccgaag ccctggtggc gacgttcccg
     1141 gtcggtgaag aaggggacca gttgggccag ccagcgatgg gcacaggcct cgatgtgtgc
     1201 gggcgaaaac tggtcggcca gttcctgcag ccgcgtcggg tcggcgcagg tccggaaggc
     1261 gttggcgcac tgctcgaagg cgatgccctc ctcacgcagc cggcaggcca gccactcgtg
     1321 gccgttgagg cagacgcggg cgctgaaagg gaagtagggg cagacccgta ggaacatccg
     1381 gccgaagtcc cggtcctgga ggtagaagta gtattgctga acgaagcggt tgcagagttc
     1441 gacgtgattg ccctgacgcg ggaagctgac ggccacgcgg gcattctcgc ggctcttgag
     1501 gatgacggcg atggcgttct ggccctgcag cttctgatag tagggttcga cccactcctc
     1561 gcggcggacg cccttgggag gctggacgat ttcgacatgg cgttggtcgg cgagggcctg
     1621 gacgaagtga tggtagtcgg tggagatggc cttgaagtag gcgggggtca cctgggtggc
     1681 ttggcgtttc tccttgagga agccgacgag gcacgcgggg ttctgcagga cctgaatgac
     1741 gccgttgagg aggatgcggt cgaagcagga gtagtcgaag cggatggcct gctggtggtg
     1801 ctcgatgaag gcgttcatgg cggacctccg aagatcaggt gggcgttgcc cacgatgata
     1861 gtgaggtccg caggcttcgg gggatttcaa ggggcacggc cccttgccta gaactagttt
     1921 gcggtgcgaa atgagggggg gcagaatcgg ccggatcgtc cctcatcctg ccgttcgcga
     1981 ccgcctcact cggcc
//
LOCUS       DBNGZC010000668         1669 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1719951, whole genome shotgun sequence.
ACCESSION   DBNGZC010000668 DBNGZC010000000
VERSION     DBNGZC010000668.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1719951"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="AC16G0_11600"
     CDS             complement(<1..666)
                     /locus_tag="AC16G0_11600"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5100291.1"
                     /translation="MLHRHFITRQITSAPRQSVVFVLCVILSMVSLVALGGFSGSVNR
                     SMLRDARKLHAGDIILHSHAEFSSGLARAVDQLTRTGAAKAARVWEFYSVVRAEGETA
                     SLLANVKVVGQGYPFYGTVVLESGRPFAQVLTPGTVVVERSLLDRLNLKVGDRLHVGQ
                     ATPTIADVVLQEPDRPVRFFSLGPRVFLPAADLADLDLVKSGSRVDYSVLLAIADETA
                     LDET"
     gene            complement(656..1339)
                     /locus_tag="AC16G0_11605"
     CDS             complement(656..1339)
                     /locus_tag="AC16G0_11605"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5100292.1"
                     /translation="MNVLEAIEITKVYSIGDRRIPVVDHVSLSVEESEFLVVKGSSGS
                     GKTTLLSLLSGLDKPTTGRVRVLDTDITDMGEDALAPLRNEVIGFVFQSFHLIPSLTA
                     LENVMFPAELKRDRAAQAKAQGLLQRVGLSERGSNYPHQLSGGEKQRVAICRALINDP
                     TIIFADEPTGNLDSSNGEAILDLLLEIHRERRITLLLVTHNSEIAGRADRVITLQDGK
                     ITLERRRAA"
BASE COUNT          319 a          532 c          520 g          298 t
ORIGIN      
        1 agtctcgtca agggccgtct cgtccgcaat ggcgaggaga actgagtaat ccacccggct
       61 cccagatttc accagatcca ggtccgccaa gtccgccgca gggagaaaaa ccctgggccc
      121 gagggaaaag aacctcacag gacgatccgg ctcctgcagc accacgtccg cgatggtggg
      181 tgtcgcctgc cccacgtgca gccgatcccc cactttgagg ttgaggcgat caagcagaga
      241 ccgttcgacc acgacggtcc cgggggtcaa cacctgggca aagggtcttc ccgactccag
      301 cacgaccgtt ccgtaaaagg ggtagccctg ccctaccacc ttgacgttgg ccagaaggga
      361 agccgtttcc ccctcggcac gcaccaccga atagaattcc cagacccggg cggccttggc
      421 agcacccgtg cgtgtgagct ggtcgaccgc ccgagccagg cccgaagaga actcggcgtg
      481 cgagtggagg atgatatctc cggcgtgcag cttcctggcg tccctgagca tggagcggtt
      541 gacgctgcca ctgaagccgc cgagggccac caaagagacc atggagagga tcacgcagag
      601 gacaaagacc acggattgcc ggggggcgct ggtgatctgc cgggtgatga aatgtctatg
      661 cagcacgtcg tctctcaaga gtgatctttc cgtcctgcag ggtgatcacc ctgtcggccc
      721 gtccggcgat ctcggagttg tgggtgacga gcagcagggt aatccggcgc tcccggtgga
      781 tctcgaggag cagatccagg atggcttcgc cgttgctgga gtccaggttg ccggtgggct
      841 catccgcaaa gatgatggtc ggatcgttga tcagggcccg acagatggcc acgcgctgct
      901 tctctcctcc cgacagttga tgcggataat tgctgccacg ctcactcagg cccacccgct
      961 gaaggaggcc ctgcgccttc gcctgagccg cccgatcccg cttgagctcg gcggggaaca
     1021 tgacattctc cagcgcggtc agggacggga tgagatggaa ggactggaaa acgaatccga
     1081 tgacctcgtt gcgcagcggg gcgagggcat cttctcccat gtctgtgatg tcggtgtcca
     1141 agacccgcac ccgtccggtg gtgggcttgt cgagcccgga gaggaggctc aacagcgtcg
     1201 tcttcccgct tccgctgctg cctttcacca caagaaactc ggactcctcc accgataggg
     1261 agacgtggtc cacgaccggg atgcgccggt ccccgatgga gtaaaccttg gtgatctcta
     1321 ttgcctccag gacattcatt caagagtcct cgctgcgacc agtctccact tcgatccgtg
     1381 cttcatcttt cattccagcc gcatgggaga tcgcggtccc ttccttctcg ggcgaaatgc
     1441 gatcccgcat tcctgccgcg tttgaccgcg cccccccggg aacgtatcag ggatgatcga
     1501 gaaaggacca cgccctggga gaccgtgggg tcattcgcgg acgcgaagac gcatccggag
     1561 ctggggcgcg ccgctccacc tggggaatgc agtggagtac ttcgcgtcac acgtgcatcg
     1621 caccgcacac gggtgcgggc caatccagtg cggtgcccgc ggcaacgcc
//
LOCUS       DBNGZC010000669         1073 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2501234, whole genome shotgun sequence.
ACCESSION   DBNGZC010000669 DBNGZC010000000
VERSION     DBNGZC010000669.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2501234"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..548)
                     /locus_tag="AC16G0_11610"
     CDS             complement(15..548)
                     /locus_tag="AC16G0_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HIR5100293.1"
                     /translation="MADPKKPIKLPETEDGAVIYNVPFPQDLTALKGMAPIITKSHYE
                     IDYIHSYGQDSPFFAGLANGKLLGTRCPQCPYTYGTPRRHCMYCGGECDWFELPTEGF
                     VHTFTVCHYGSQEFLPETPFVLILVEWPGVDTLFLSRLVGVDSAAPSLDWVGMKVSAR
                     FKRLSKFKPTDVYFVPA"
     gene            complement(551..>1073)
                     /locus_tag="AC16G0_11615"
     CDS             complement(551..>1073)
                     /locus_tag="AC16G0_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR5100294.1"
                     /translation="EVMLLPHEKAEDYRGLKYPGVHSFRAGRMASKTAYQRAGIGNPL
                     EDLDFIELHDAYTSSEIQTYEDMGLCRYGEGGKFVEAGKPFLPGVDYGYRFPEQGKIP
                     VNPSGGLIACGHPVGATGLMQAVFALWQLQESIGRHFGDPTLQVKAARRGAIHSHAGT
                     GTYVVVTILERGF"
BASE COUNT          201 a          348 c          325 g          199 t
ORIGIN      
        1 accctttctg gtgcctaggc cgggacgaag tacacgtcag tcggcttgaa cttggacagc
       61 cgcttgaacc gtgcactcac cttcatcccc acccagtcca gggacggcgc cgctgaatcc
      121 acgcccacca gcctggagag aaacaaggtg tctactcctg gccactccac caggatgagg
      181 acgaacggcg tctccggcag gaactcctgg cttccgtagt ggcagacggt gaaggtgtgg
      241 acgaatccct ccgtcggcaa ctcgaaccaa tcgcactcgc caccgcagta catgcagtgc
      301 cgccgcggcg tcccgtaggt gtaaggacac tgcggacacc gggtccccag cagcttgccg
      361 ttggccagcc ccgcaaagaa gggcgaatcc tgaccatagc tgtgaatgta gtcgatctcg
      421 taatgggact tggtgatgat cggggccatg cctttcaggg ccgtgagatc ctggggaaaa
      481 ggcacgttgt atatgaccgc cccatcttcc gtctcgggga gcttgatggg cttctttgga
      541 tcggccatgg ctagaacccc ctctccagga tcgtgaccac gacataggtt ccggtgcctg
      601 catggctgtg gatggcgccg cgacgcgcag ccttcacctg caaggtggga tccccgaagt
      661 ggcgcccgat gctctcctga agctgccaca gggcaaagac cgcctgcatg agccccgtgg
      721 cgcctacggg atgcccgcag gcgatgagac cgcccgaggg attcacggga atcttccctt
      781 gctccgggaa gcggtagccg taatccacgc cgggcaggaa cggcttcccc gcctctacga
      841 actttccgcc ttcgccgtag cgacacagcc ccatgtcctc gtatgtctga atctcggagg
      901 aggtgtaggc gtcgtgcagc tcgatgaagt ccagatcctc cagcgggttc ccgattccgg
      961 ccctctggta ggccgtcttg gaggccatac gaccggcccg gaaggagtgg acgcccgggt
     1021 acttcaagcc ccggtagtcc tcggccttct cgtgcgggag gagcatcacc tcc
//
LOCUS       DBNGZC010000670         5504 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_58957, whole genome shotgun sequence.
ACCESSION   DBNGZC010000670 DBNGZC010000000
VERSION     DBNGZC010000670.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_58957"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /gene="ygeW"
                     /locus_tag="AC16G0_11620"
     CDS             <1..1113
                     /gene="ygeW"
                     /locus_tag="AC16G0_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HIR5100295.1"
                     /translation="TLEKTDDELAAVFAVAEILRGMRRNNVSARIFDSGLAVSIFRDN
                     STRTRFSFASASNLLGLTLQDLGEGKSQIAHGETVRETAVMVSFMAEVIGIRDDMYIG
                     KGNAYMREVSRAVVESHAAGILERRPTLVNLQCDIDHPTQSMADALHLIQHFGGAEKL
                     RGKKIAMSWAYSPSYGKPLSVPQGVIGLMTRFGMEVVLAHPPGYEVMQETMDVAKRNA
                     ARTGGSFEVTHDMRQAFRGADIVYPKSWAPYAAMEKRTDLYGRRDMGGIQDLEKELLA
                     QNARHKDWECTEKLMEETKGGRALYMHCLPADISGVSCKEGEVAASVFDRYRDPLYRE
                     ASCKPYIIAAMIFLSKVEHPADMLSRLLTAGRPRVL"
     gene            1902..2120
                     /locus_tag="AC16G0_11625"
     CDS             1902..2120
                     /locus_tag="AC16G0_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100296.1"
                     /translation="MIETILYVGGWTLFFLFLYFYVGWIGLERRFFAGEDPEDWGSHF
                     RAEEVPLDSQQPEPSMRLSFLPSLGRPA"
     gene            complement(2468..3112)
                     /locus_tag="AC16G0_11630"
     CDS             complement(2468..3112)
                     /locus_tag="AC16G0_11630"
                     /inference="COORDINATES: protein motif:HMM:NF012401.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR5100297.1"
                     /translation="MHTRRSFLALALMNTLGGGLLLDLLASPMEWAWAASRKVLGKDT
                     KRESLIHENPAALDPTNLAITPLEQFGTMGPIDRVVDLATWRLEIAGQVRRPLLLPYV
                     QLTALPPIEREVLLICPGFFANHGRWRGVSLQGLLDQADFDRTASRVTIEAKGEKGIS
                     FPLTEVLAERVFLAYQVNGQALPRKHGFPLRVVAEDHYGSDWVKYVDRITVERS"
     gene            3463..4116
                     /locus_tag="AC16G0_11635"
     CDS             3463..4116
                     /locus_tag="AC16G0_11635"
                     /inference="COORDINATES: protein motif:HMM:NF015682.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HIR5100298.1"
                     /translation="MHPREICERYERLYTGAVADILDEQGFRRQCLGKELRPLLSGRR
                     AAGFAYPALGAACADVGYDCRPEILKFLGEIPADSMVVFDPGAEKVCAAWGEFMALAA
                     RNNGGRGVVVDGAIRDAEMIVQADFQIFHIGFWPVSSVGRWRPVHAGHPILFHGVEVR
                     PGDLVLGDIDGVVVVPRPLVEEVLVAAEGCYQAERSMKEELRAGKTMEEVFRRYGRW"
     gene            4238..5299
                     /locus_tag="AC16G0_11640"
     CDS             4238..5299
                     /locus_tag="AC16G0_11640"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR5100299.1"
                     /translation="MSTPNFERFRTVLLLRGEPDYVPLGDIGIHPLLKAGVLGRPVRS
                     LEDEVAFWATAGYDYVPLEQGLQLTDIIQQKSMREVEAQYAADTSDTQVRSWATEGQG
                     LITCRADLEALQWPDPDSLDYTGFDKLGRLLPANIQVIAVLGKVFTCVSWLMGLEGLS
                     FALADDPGLVAALFDRVGRFQSRVLERMLEFPCVGAVWHADDIAFKTQLLVNPRVLRA
                     HVFPTYTAMNRLTRQRGRLAVYHSDGALQEVVEDIIGCGFHALNPIEPPAMDITAIKR
                     RYGSHLAILGNIDLGYTLTRGTPDEVRAEVRQRIHDLAPGGGYAVASSNSVPEYVPLA
                     NFNAMREATFEYGRYPIQV"
BASE COUNT         1001 a         1745 c         1776 g          982 t
ORIGIN      
        1 accttggaaa agaccgacga cgagctggcg gcggtcttcg ccgtcgcaga aatcctgcgg
       61 ggtatgcgtc gaaataacgt ctcggccagg attttcgaca gcggtctggc cgtgagcatc
      121 ttccgcgaca actccacccg cacccgcttc agcttcgcca gcgccagcaa cctcctgggt
      181 ctcacgctcc aggacctggg cgagggcaag agtcagatcg cccatggcga gacggtgcgg
      241 gaaacggccg tcatggtgtc cttcatggcc gaggtgatcg gcatccgcga cgacatgtac
      301 attggcaagg gcaacgccta catgcgggag gtgtcccgcg cggtggtgga aagccatgct
      361 gccggcatcc tggagcggcg ccccacgttg gtgaacctcc agtgcgacat cgatcatccc
      421 acccagtcca tggccgacgc cctgcacctg atccaacact tcggcggcgc ggagaaactc
      481 cgaggcaaga agatcgccat gagctgggcc tactcgccct cctatggaaa accgctctcc
      541 gtgccccagg gcgtcatcgg cctgatgacc cggttcggca tggaggtggt gctggcccat
      601 ccgccgggct acgaggtgat gcaggagacg atggacgtgg ccaagagaaa tgccgcccga
      661 actggcggat ccttcgaggt cacccacgac atgcgccagg cgttcagggg tgcggacatc
      721 gtctatccca agagctgggc tccctacgcg gccatggaaa aacgcaccga cctctacggc
      781 cggcgagaca tgggcggcat ccaggatctg gaaaaggagc tgctggctca aaacgccagg
      841 cacaaggact gggagtgcac cgagaagttg atggaggaga ccaagggggg ccgcgccctc
      901 tacatgcact gccttcccgc cgacatctcc ggcgtgagct gcaaggaggg cgaagtggcc
      961 gcctccgtct tcgaccgcta ccgggacccg ctctaccggg aagccagctg caagccctac
     1021 atcatcgcgg ccatgatctt cctgtcgaag gtcgagcatc ctgcggacat gctcagccgc
     1081 ctgctcacgg ctggtcggcc gcgggtcctc tagcatccga gcaggccggt atctggaagg
     1141 tcggctggta cgagcgcctg ataaagcccc ccgaagacaa aaccctcacg gccccaggcc
     1201 atgtctggaa cgatcccgcc accaccgcca aggcggccgc caccctgccc cgcagtcagt
     1261 accttgggat ggtgaacaac acccaagaga aaaccgggct gctggtgttc aacggtacct
     1321 ctgtcggacg gctccaggcc gcgccggggg gaatcctatc aaagccgccg agattctcgg
     1381 cccgcggcgc gcctacctct cccggctgct aaaggcgctg gacacctgtc agccctcgta
     1441 ataggagtcc actccggatc tcgccagcag ctttctcctg tatccgtttg tgaccatcgc
     1501 ctgtatcttg ttcgagtcgg tccatgtcct aggcagccct ctgatggaag gctcaacccg
     1561 ccaagattcg ccgcaacgac aaacacttac gaccgtgtca acatcaggcg tctgctcggc
     1621 atggatcctg ctttctacgg aggacattca aatgatggat gtggaatcac ccaacggttc
     1681 gagcggtaag tgactacgca ggagcaagct ggaggggcag cggtcgccct catgcaccgg
     1741 ccccgcgaag gctgggtcga gcgagttggt cgttggagaa tgtggttagg catactttgg
     1801 attcgatgct ggggaaagag caggtctccg ttcctggcct ggttgtaggg gaggcggttc
     1861 gtcgcggacg aaggtcggtc cgaggaagga ggctggaggc catgattgag acgatcttgt
     1921 acgtcggagg ctggacgctg ttcttcctgt tcttgtactt ttacgtgggc tggattggct
     1981 tggagcggag attttttgcc ggagaggatc cggaggactg gggctctcat tttcgggctg
     2041 aggaggtacc cttggattcc cagcagcccg agcccagcat gcggctctct tttctcccct
     2101 cgctgggtcg gccggcctaa agagctgagg ccgcagcgag tcgcgacccg gacgcccaga
     2161 gggtctccac gactacaaag ggagatggga cgctaaaaga catcgccgct gagttactcc
     2221 ggcctccatt ccgccctcgg gggcgggagc caggcatggc cgaagctacc aatgaaccca
     2281 aagagtggta gcgtaactta gcggcgatga ccctcactgt aaccttcttt ttcccaacca
     2341 gcaagcacac cccgacttct atcacacccc catctaagcg cagcgggtgc ggatctgcct
     2401 cacgagcatg ggtcggagag aatggcgaaa ggtttgtgcg tcgcaggggt gacctgccgg
     2461 cagaaaccta cgaccgctct acggtgatgc gatcgacata ctttacccaa tccgacccat
     2521 agtgatcctc ggccaccacc cggaggggga acccatgttt ccggggcagg gcctggccat
     2581 tcacctggta ggcgagaaag accctttccg cgagcacctc ggtcagggga aagctgattc
     2641 ccttctcacc tttggcctcg atggtgaccc gggacgccgt gcggtcgaag tccgcctggt
     2701 ccaggagtcc ctgcagggag acaccgcgcc agcggccgtg attggcgaag aagcctggac
     2761 agatcaagag cacctcccgc tcgatggggg gcagggcggt gagctggaca tagggcagga
     2821 gtaaaggccg cctcacctgg ccggcgatct ccaaccgcca cgtcgcgagg tcgacgaccc
     2881 gatcgatggg gcccatggtg ccgaactgtt ccaggggagt gatcgccagg ttggtcggat
     2941 caagggcggc ggggttttca tggatcaagc tctcgcgttt cgtatccttg cccaacacct
     3001 tccgactcgc ggcccatgcc cactccatcg gggacgcgag gagatccagc agcaggcccc
     3061 cgcccagggt attcatgaga gccagcgcga gaaagcttct ccgggtgtgc atggatcctc
     3121 tcccttctag acatgatggc tcctgcggac ggctccgccg gccgccgcgg attcgggccg
     3181 gacgaacgag gacgggagga agcctccgag ggaacagctt cctggatcgc gtttgccgct
     3241 gcggccttcg gccgtggtct gtggtcggaa tttcctcacg ggcataagat acctcctctt
     3301 gtttccggtg tctcgcatga atcgtggcgt cggatcacgc aaccatgggg tcgacccccg
     3361 ccgatcctca tgagtgtcca gcctgtggag aatgtcattg tcaccagggg cggcctgtga
     3421 taagcggaag ctctcaacct ggacggaacg ggaggttagc gtatgcatcc gcgagagatc
     3481 tgcgagcggt atgaaaggct gtacacgggg gccgtggccg acatcctcga cgagcagggg
     3541 ttccgccggc agtgcctcgg gaaggaactc cgtccactct tgtcaggcag gcgggcggcg
     3601 ggattcgcct atccggccct cggcgcagcc tgcgcggatg tgggctacga ttgccgcccc
     3661 gagatcctga agttcttggg ggagatccct gcggattcca tggtggtgtt tgatccgggc
     3721 gcggagaagg tctgcgcggc ctggggcgag ttcatggcgc tggcggcgcg aaacaacggc
     3781 ggccgcggcg tggtcgtgga cggggcgatc cgggacgccg agatgatcgt gcaggcagat
     3841 ttccagatct tccacatcgg cttctggccg gtttcctccg tggggcggtg gcgtccggtg
     3901 cacgccggac acccgatcct cttccacggc gtcgaggtcc ggcccggcga cctggttctc
     3961 ggcgacatcg acggggtggt cgtggtaccg cgaccgctgg tggaggaggt gcttgtggcc
     4021 gccgagggct gctaccaggc cgagcggagc atgaaggagg agctgcgcgc gggcaagacc
     4081 atggaggagg tcttccggcg gtacggccgc tggtaatcgt cccggctccc atggcaacgc
     4141 cccaggcgac ccagcacctc cgcgaatgcg gccgaaatcg tgaaaacgtg aagaggcccg
     4201 caccggtcca ggggattcac cggacgaaag gaggctcatg agcacgccca acttcgaacg
     4261 cttccgcaca gtcctgctcc tccgggggga gccggactat gtccccctcg gagacatcgg
     4321 catccacccc ctgctgaagg cgggcgtgct cggccgcccg gtccggagcc tggaggatga
     4381 ggtggcgttc tgggccacag ccggatacga ctacgtgccc ctggagcaag ggctgcagct
     4441 cacggacatc atccagcaga aatccatgag ggaggtcgaa gcccagtatg ctgcggacac
     4501 cagcgacact caggtccgct cttgggcgac cgagggccag ggtctgatca cctgccgggc
     4561 ggatcttgaa gccttgcagt ggcctgaccc cgacagcctg gattacaccg ggttcgacaa
     4621 gctgggccga ctcctcccgg ccaacatcca ggtcatcgcc gttctgggca aggtcttcac
     4681 ctgcgtctcc tggctcatgg gattggaggg tctgagcttc gccttggccg acgatcccgg
     4741 cttggtggcg gcgctcttcg atcgggtggg gagatttcag tcccgtgtgc tcgaacgcat
     4801 gctggagttc ccgtgcgtcg gcgcggtctg gcacgcggac gacatcgcct tcaagaccca
     4861 gctcctggtc aatccccgcg tcctgcgggc gcacgtcttt cctacctaca cggccatgaa
     4921 ccggctgacg cggcaacggg gccgcctggc ggtctaccac agcgacggag ccctccaaga
     4981 agtcgtggag gacatcatcg gctgcggctt ccatgcgctg aatccgatcg agccgccggc
     5041 catggacatc accgcgatca aacggcggta cggctcccac ctcgccatcc tggggaacat
     5101 cgatctcggc tacacgctca cccgcgggac gccggacgag gtccgggccg aggtccggca
     5161 gcgcattcac gatctcgcgc ccggcggggg ctatgccgtg gcctccagca actccgtccc
     5221 cgaatacgtc ccgctggcga atttcaacgc catgcgcgag gccacgtttg agtacggccg
     5281 ctatcccatt caggtctaac tgagagggag gggcggaccc gatcccaagc cggcttcccg
     5341 cccgctctga ttgggattgg tctcccaccc ggcccgccgc acctgcaggc tcgccgtgat
     5401 ttcccttgac ccgtcattct gccggtggta gcgtcagcag cctacgctgg gtttcccccg
     5461 ggggtcgtcg ctggttcggc cgtccggcgg ttacattcca cttg
//
LOCUS       DBNGZC010000671         2145 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2406280, whole genome shotgun sequence.
ACCESSION   DBNGZC010000671 DBNGZC010000000
VERSION     DBNGZC010000671.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2406280"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="AC16G0_11645"
     CDS             <1..248
                     /locus_tag="AC16G0_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011228608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5100300.1"
                     /translation="NAMIPVLTVFGLEFGYMLGGAVLTETVCSLPGIGRLLVEGIFSR
                     DYPVVQGAMMVVATTFVLVNLLTDVAYAFFDPRIRYE"
     gene            407..1228
                     /locus_tag="AC16G0_11650"
     CDS             407..1228
                     /locus_tag="AC16G0_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5100301.1"
                     /translation="MWRRLRRNRAAVAGAGIVLAFVLLAVLAPVLVPYSPIQGDLNDR
                     LQSFSPTHWLGTDELGRDLLSRILYGARVSLQIQIVAVVLALLIGVALGSMSGYLGGL
                     VDNLIMRSMDVLLAFPGIFLALGIIAALGPGLLNLMFAAGISSIPQFARIVRASILTL
                     KEREFVEAALALGSGSNRVMFRHLLPNCMAPIIVQSTLRMATVLLTASGLSFLGLGVQ
                     PPTPEWGAMLSNARSYLIVAPHVATIPGLAIMVVVVGFNLFGDGLRDTLDPRLRQ"
     gene            1714..>2145
                     /locus_tag="AC16G0_11655"
     CDS             1714..>2145
                     /locus_tag="AC16G0_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100302.1"
                     /translation="MGVLFGLTLAIFAAVPSPGLGAQFDLELFAPARDAERAILAARR
                     AGPADSAGQDLSLATHYFEEAKAALQPASGLPNREKATHLFRLAEGHARLAEARTVEE
                     TRNHEAGDAALQYLQAINGAPPGMTPTGPSPSEAAASFSRLR"
BASE COUNT          359 a          679 c          680 g          427 t
ORIGIN      
        1 agaacgccat gatccccgtg ctcacggtct tcggcctgga attcggatat atgctgggtg
       61 gggcggtact gaccgagacg gtgtgttctc tccccgggat cgggcggctc ctggtggagg
      121 ggatcttctc gcgggactac ccagtcgtcc agggtgctat gatggtggtc gcgacgacct
      181 tcgtgctggt gaacctgttg acggacgtgg cgtacgcgtt cttcgatccg cgcatccggt
      241 acgagtgaaa tcccaatgac caaagacaaa atcccaatgg aataccttcc cccatgggtc
      301 atttgggttt cgtcattggt cattcgacga gaagcatgag atggaaacac cagcgacccg
      361 aggtgaagga gttgcaccga gcgcgtctcg cggtgagctg gccgacatgt ggcgacgctt
      421 gcgacgaaac cgggcagccg tggccggggc cggcatcgtg ctggctttcg tgctcctggc
      481 ggtcttggcg ccggtcctgg ttccttacag tcccatccag ggagacctga acgatcgcct
      541 gcaatcgttc agccccaccc actggctcgg gacggacgag ctgggccgtg acctcctgag
      601 tcgcattctg tatggcgcca gggtatcgct gcagatccag atcgtcgccg tggttctggc
      661 cctcttgatc ggcgtggccc ttggctcgat gagcgggtac ctgggtggcc tcgtagataa
      721 tctcattatg cgatccatgg atgtcttact agcctttccg gggatttttc tggccctagg
      781 gatcatcgcg gccttggggc cgggcctgct taatttgatg ttcgccgcgg gtatcagttc
      841 gatcccgcag ttcgcccgga ttgtgcgcgc ctccatccta accctcaagg agcgggaatt
      901 cgtggaggcc gccctggcgc tcgggagcgg cagcaaccgg gtgatgttcc gtcacctcct
      961 gcccaactgc atggccccaa tcatcgtcca gtccaccttg cgcatggcga cggtcctcct
     1021 gaccgcctcc gggctgagct tcctcggtct gggggttcag ccgccgacgc cggagtgggg
     1081 cgccatgctg tcgaacgcgc gaagctacct catcgtagcc ccccacgtgg ccaccatccc
     1141 cggcctcgcc atcatggtcg tggtcgtcgg cttcaatctc ttcggcgacg gtctccgcga
     1201 caccctggac ccccgcctgc ggcagtaagg gccgtggctt tcctcgtgcg accgaaggtt
     1261 ctggaaagca tttcgtcgct gaaacggtgt catttttaca cacgcctctg agtataagtc
     1321 gttttgaacg tctacctgcg agtgcaggcg cggtcggcct aactttctgt cattcttaag
     1381 gatacgtgat ccggtcgaca accaatcaaa ccttggcacc ggccttgctc cctttcaacc
     1441 ggaggcgggc aacagccgcc gggaagtatc cctgtccagc cgcgaggagc ggaactagcc
     1501 gatgcggcgc atgtcatact gtgcggtgcg caaaagaggc gacggttcca ccacggccgc
     1561 acagtccggg ggagagtccg aggaggggcg gcccgcccct cacggtcggg tacccgcgcc
     1621 acaggcgtgg gtgcgcgggg aacggtgtag ccattattcc gcgctgcgga gtaactgggc
     1681 aggcatgacg ggagcagggt aatgagacag gggatgggag tgctgtttgg attaaccttg
     1741 gcgatcttcg cggccgtccc gtcgccgggc ttgggggccc agtttgatct cgagctgttt
     1801 gctccggccc gcgacgccga gcgggccatc ctggccgcac gtcgcgccgg gcccgccgac
     1861 tctgctggcc aggatctaag tctggccacc cactacttcg aagaggccaa ggccgctctc
     1921 cagccggcct ccggcctacc caacagagag aaggccaccc acctgttccg cttggcggag
     1981 gggcacgcta ggctcgccga ggctcgcacc gtcgaggaaa cacgcaatca cgaggcgggc
     2041 gatgcggccc tccagtatct gcaggccatc aacggggcac ctccagggat gacacccaca
     2101 ggtccgtcac cgtccgaggc agcagcttca ttcagccgcc tccgg
//
LOCUS       DBNGZC010000672         4756 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1232843, whole genome shotgun sequence.
ACCESSION   DBNGZC010000672 DBNGZC010000000
VERSION     DBNGZC010000672.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1232843"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..878
                     /locus_tag="AC16G0_11660"
     CDS             <1..878
                     /locus_tag="AC16G0_11660"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5100303.1"
                     /translation="PAWATAMPATASFPRVALAHVFPGTNQTEAQVQAVQEAEAYLAV
                     AVQPLLAGGIKAQTVVRYGHAAEEILDHAEAGSVDLIAMSTHGRSGPRRWMLGSIAEK
                     VLRAAPVPVLLVRARGELAVEAVSEARQRLVPAASKEIQFVPAAIHHILCPVDFSQQS
                     HEIAEYAGVLALRFSADLTVLHAVYDPLDITCSHIPHPPLEQLREEMIRVAEHTLQRL
                     VNRKLRRLPRAKAVVVAGPPGQQITRYAREHGVDLIVMGTQGLSGLNHLILGSTAERV
                     IRYAPCPVVSIRAAA"
     gene            952..1596
                     /locus_tag="AC16G0_11665"
     CDS             952..1596
                     /locus_tag="AC16G0_11665"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HIR5100304.1"
                     /translation="MQVEKRMKANPITVSPEDSFRHAMTLIRQRGIRHLPVVEEGRLV
                     GIVTDRDLRQASPSQATSLESHELLYLLEKLKVRDIMTAKVYTVAPETPIEEAARLML
                     QHKIGGLPVLRAGMLIGILTETDILRAFVDLTGLQADQTRLELVLEDRPGAFLDVCRI
                     IQDQGGDFASMVSATLPHGGQDKKVMVFRLEGVQAEPLVKRLEQSGHTVLSITT"
     gene            2004..4115
                     /locus_tag="AC16G0_11670"
     CDS             2004..4115
                     /locus_tag="AC16G0_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HIR5100305.1"
                     /translation="MPGTQVDYTTTKNLLQSFGISIIGTPVRTREEAVATATEIGFPC
                     VMKLISPDVIHKTDVGVVILDIASASTAGKSYDTIVANGRKAGAQRIDGVLVQKQARP
                     GFELLVGARQDPVFGPVTMIGYGGRYVELFKEVAPGVGVLTRADVERMLSETVVSRVI
                     AGFRGPALDKDAVIDLTIRVSQLMEARPEVTELDLNPVILYPKGCCIVDARMIVGEPI
                     HHPRAVHLSETRLRSLKAIFEAKSVAVVGASSPGSIGGIILKNSSRVERLYPINPKRD
                     TLMGLKCYKGFHDLPTPADVAVFAVAPETTIASFRQFCETGGKGAIVVSDGFAEIGRS
                     DLEDELRAISEQHGVVYIGPNCLGVFDNFSGLNTLFLPMRSLNTNTKPGLVGVVSQSG
                     GVGLELLEMAAADNLAIGKWVSCGNASGISIPELLAHMGDDRRIRVIAIYVEGLRNGL
                     QFMEVGRKIAKRKPVIVIKGGMAGGAAATMSHTASLAGSFQAFKAACHQAGFYLIEEL
                     TEDPKILINVLSILTTQKPARNNRVAVVSVGGGAAILLADQITSNGMQLTTFAEDTQS
                     KLARLLGSKIRAASDADRARIADRVASNPLDLFGDADDDRLLEAIRILNDDQNTDVIV
                     VGLYFQVPYLSEYIAERLVEVMREMRKPLILSARGYSHFVQQTTEYLAQHDVHTYTVP
                     MIKPLAIALDIWKRYDLVFTA"
     gene            4178..4582
                     /locus_tag="AC16G0_11675"
     CDS             4178..4582
                     /locus_tag="AC16G0_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100306.1"
                     /translation="MKRTYFCPTCLATLNPNVKLILAMAKGRRRSLILLSPRPGDYSV
                     ILPSEVSLRPGDEVQFFCPACGLNLRSEANPHLAEIGFRLEDGTRGRVNFSRIFGEHA
                     TYFVTKEQIRSYGENAAIYGDANFFGAGGERG"
BASE COUNT          845 a         1578 c         1481 g          852 t
ORIGIN      
        1 cgcccgcatg ggcgacggcc atgcccgcta cggcgtcgtt cccgcgggtg gctctggccc
       61 acgtcttccc tggaacgaat cagaccgagg cacaagttca ggcggttcag gaggcggagg
      121 cctatctggc cgtcgcggtc caacctctcc ttgccggcgg gatcaaggcc cagactgtcg
      181 tgcgctacgg ccacgccgcc gaggagatct tggaccacgc cgaggccggt tccgtggatc
      241 tcatcgccat gtccacccat gggcggagcg ggccccggcg atggatgctg ggaagcattg
      301 ccgagaaggt ccttcgggcg gccccggttc cggtcctctt ggtccgagcg cggggggaac
      361 tggcagtcga agcagtctcc gaagcacgtc agcggcttgt gccggccgcc tcgaaggaga
      421 tccagttcgt cccggccgcc atccaccaca tcctgtgccc agttgatttc tcccagcaat
      481 cccacgagat cgcggagtac gcgggtgtcc tggccctccg atttagcgcc gacctgacgg
      541 tcctccacgc ggtctacgat ccgctggaca tcacctgctc tcacatcccc cacccgcccc
      601 tggagcaact gcgtgaggag atgattcggg tggccgagca cacactccag cggctggtga
      661 accggaagct ccgtcgcctc ccgcgtgcca aggcggtcgt ggtcgcaggt ccaccgggcc
      721 agcagatcac tcgttacgct cgcgagcatg gggtggacct gatcgtaatg gggacacagg
      781 ggctctccgg tctgaatcac ctgatcctgg gcagcacggc cgagcgggtc atccgctacg
      841 ccccgtgtcc tgtcgtcagc atccgcgccg ccgcctagat cgttcggccg ggcgtcaggc
      901 ccccgcctgc gctgggcagc cacccacgat ggcagcccga ggaggaatgc catgcaggtc
      961 gaaaagcgga tgaaggccaa cccgatcacg gtgagccccg aagattcgtt tcgacacgcc
     1021 atgaccctca tacgtcagcg gggcatccgc cacttgcccg tggtggagga aggtcgactg
     1081 gtcggcattg tcaccgatcg ggaccttcga caggcctcgc cctcccaggc taccagcctg
     1141 gagagccacg agctactcta tctgttggag aagctgaagg tccgcgacat catgaccgcc
     1201 aaggtgtaca ccgtggcgcc agagaccccc atcgaggagg cggcgcggct gatgctccag
     1261 cacaagatcg ggggcctgcc ggtcctccgg gcggggatgc ttatcggcat cctcaccgag
     1321 accgacatcc tgcgggcctt cgtggacctg actggcctcc aggcggacca gaccagactc
     1381 gagttggtcc tggaggatcg gccgggtgcc ttcctggatg tgtgccggat catccaggac
     1441 caggggggcg acttcgccag catggtgagc gccacgctcc cccatggcgg tcaagacaag
     1501 aaggtcatgg tcttcagact cgagggcgtt caggcggagc ccttggtgaa gcgcctggag
     1561 cagagcggcc acaccgtcct gtccatcacc acctgacggc ggtttcccac cggggatccg
     1621 agcagcactc cagccagggg gtcgatccgg accggcggcc aattgcctct ctgcctcggt
     1681 ttctgctcgt ggcacgctat gcttggctgt tcccccgcct gccacaggcg cgcaacccgc
     1741 tgccggtcgc acccgccatc gtcgccggag cggatatcgt ccataccttg tggcgagcac
     1801 gcgggtctag acgtcggact tcatcggcgc gatggtggcc gtatggcgtg ctcccggcgc
     1861 atattggcgt cacgccgagc gcgacgaccg agggcaaggc ccgcgacatc atggcagctg
     1921 cctgcttggc ggaggagtga ggagggtcga ggctgcctca ttcaggggca cggcgcatga
     1981 cagcgccact cgaggagacg accatgcctg gcacccaggt cgattacacc acgaccaaga
     2041 acctgctcca gtcctttggc atctccatca tcggcacccc ggtccgcacc cgtgaggaag
     2101 ctgtggcgac cgccacggaa atcggcttcc cctgcgtcat gaagctcatc tcgccagacg
     2161 tgatccacaa gacggacgta ggtgtcgtga ttctcgacat cgcgagcgca tccacggcgg
     2221 gaaagagcta cgacacgatc gtcgccaacg gacgcaaggc gggggcgcag cgcatcgatg
     2281 gggtcctggt ccagaagcaa gccagaccgg ggttcgagct tttggtgggc gctcgtcagg
     2341 atccggtgtt tgggccagtc accatgatcg gctatggcgg acgctatgtt gaattgttca
     2401 aggaggtggc cccgggcgtc ggtgtgctga ctcgcgccga cgtcgagcgc atgctctcgg
     2461 agaccgtggt gtcgcgagtc atcgccgggt tccgcggccc cgccctcgac aaggacgccg
     2521 tcatcgacct caccattcgg gtctcccaac tgatggaggc gaggcccgag gtcaccgagt
     2581 tggatctgaa cccggtgatc ctttatccga agggttgctg tatcgtggat gcgcgcatga
     2641 tcgtcggcga gcccatccac cacccccggg ctgtccatct ctccgaaacc cggctccgaa
     2701 gtctcaaggc gatcttcgag gccaagagcg tggcggtggt cggcgcatcc agcccgggct
     2761 cgatcggcgg aatcatcctg aagaactcca gtcgggtgga acgtctctac cccatcaatc
     2821 ccaagcgcga cacattgatg ggtctcaagt gctacaaggg cttccacgac cttccgaccc
     2881 ccgcagacgt cgccgtcttt gccgtggcgc cggaaacgac catcgccagc ttcaggcaat
     2941 tctgcgagac cggcggcaaa ggcgccatcg tggtgagcga cggcttcgcc gagatcgggc
     3001 gaagcgacct ggaggacgaa cttcgggcga tctcggagca gcatggcgtg gtgtacatcg
     3061 gcccgaactg tctcggggtc ttcgacaact tctccgggct caacacgctc ttcttgccca
     3121 tgcgaagctt gaacaccaac accaaaccgg gcttggtcgg cgtggtgtca cagagcggcg
     3181 gcgtcggcct cgaactcctc gagatggcgg ccgccgacaa cctggcgatc ggcaagtggg
     3241 tgtcctgcgg caatgccagc ggcatttcca ttccggagct tctggcgcac atgggcgacg
     3301 accggcgcat ccgggtcatc gccatctacg tggaagggct gcgcaacgga ctccaattca
     3361 tggaagtcgg ccgcaagatt gccaagcgga agccggtcat cgtcatcaag ggcggcatgg
     3421 ccggcggggc ggctgccacc atgtcccaca cagcctccct ggcaggatcc ttccaggcct
     3481 tcaaagccgc gtgccaccag gcgggcttct acctcatcga ggaactcacc gaggatccaa
     3541 agatcctgat caacgtgctc tcgatcctga cgacgcaaaa gcccgcccgg aacaaccggg
     3601 tggctgtggt gagcgtgggc ggcggcgcgg ccatcttgtt ggccgaccag atcacctcga
     3661 atggaatgca gttgacgacg ttcgccgagg acacgcaatc caagctcgcc cgtctgctcg
     3721 gcagcaaaat ccgcgcggcg agcgacgccg acagggcgcg cattgcggat cgggtggcgt
     3781 cgaaccctct ggatcttttc ggcgatgccg acgacgatcg attgcttgaa gccattcgca
     3841 tcctcaacga cgaccagaat accgacgtca tcgttgtggg tctgtacttc caggtcccct
     3901 atctttccga gtacattgcg gagcgcctgg tcgaggtcat gcgagagatg cgcaagcccc
     3961 tcatcctctc ggcccgggga tactcgcact tcgtgcagca gacgaccgaa tatctggccc
     4021 agcacgatgt gcatacctac acggtcccca tgattaagcc gctggccatt gccctcgaca
     4081 tctggaagcg gtacgacctt gtttttaccg cctgatgcct ggcgtcgccg cccgcgcctg
     4141 acgccaaaac ccccgtgtgc cgaaagcgag acctgcgatg aagcggactt acttttgccc
     4201 cacctgcttg gcgacgctca acccgaacgt gaaactcatt ctcgccatgg cgaagggcag
     4261 gcgccgcagc ctcatcctgc tcagtcctcg gccgggcgac tacagtgtca tcctgccgag
     4321 cgaggtcagc ctgaggcccg gcgacgaagt ccagtttttc tgcccggcct gcggtctcaa
     4381 cctgcgaagt gaggccaacc cccatttggc ggagatcgga ttccggctgg aggacggcac
     4441 ccgaggtcgg gtgaatttct cccggatatt cggggagcac gcgacctact tcgtcaccaa
     4501 ggaacagatc cgctcttacg gcgagaacgc cgcaatctat ggcgacgcga atttcttcgg
     4561 cgctggcggc gagcgaggat aactctccgc ccccgattct cgccgcctgt tatcgccgcg
     4621 gctaccctaa ccttgagcgc tccttcgccg ctttgctccg ggctgcagtg tcggcctggt
     4681 ccttcaggga gatgtcctgt tcgcctcatt tttttcccgc cgcgcttgac ttcggtgagg
     4741 gcccagccta tgttcg
//
LOCUS       DBNGZC010000673         2944 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2113966, whole genome shotgun sequence.
ACCESSION   DBNGZC010000673 DBNGZC010000000
VERSION     DBNGZC010000673.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2113966"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            448..789
                     /locus_tag="AC16G0_11680"
     CDS             448..789
                     /locus_tag="AC16G0_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100307.1"
                     /translation="MLRPCLVAIMLGLLMLAGFPAEAAKWSRAYIRQLPDSAFAAIET
                     TPDGKTLRHLPHHDAAGNLDLPHLRSALSRLSQVKWQNPATAGSARQHLLDHMRQHQQ
                     ERGKSPLKGSP"
     gene            complement(879..2159)
                     /locus_tag="AC16G0_11685"
     CDS             complement(879..2159)
                     /locus_tag="AC16G0_11685"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR5100308.1"
                     /translation="MATSELLPLYDVIAQCNRCGFCQAGCPVFRVTGEEHSLARGRMA
                     VARALILGQLELTPEVAHALDDCLLCRGCTAHCFPALKTDEIVTAIRQANIARHGQPM
                     WQRVLFRSVLSNSKRLETAGRMALWAKRNGLATLAEKTGILNLVDKRLAVASQVVPPL
                     TAKPFLRGGGAQVPTPATVKHRIGYFVSCGFSFQFPEVVDATLRVLAQSGCAITVLDN
                     TCCGRPAQSYGDLEAARDIARRNVDRLAPAMGLDVIVSECGSCSTHLKDYGSLLKGDP
                     AYAVKATALSKKIQSFSEFLVAAGANGSLGPLQGTVTYHDPCHLSNRFAKVTAQPRKL
                     LKSIPGATYKELPEADWCCGAAGTYTFLHHAEAMGVLDRKMGNVEKTGATILATECPA
                     CMMHLAYGVKRRGLSVQVRHVSQLLDQAYAAGKA"
     gene            complement(2174..>2944)
                     /locus_tag="AC16G0_11690"
     CDS             complement(2174..>2944)
                     /locus_tag="AC16G0_11690"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5100309.1"
                     /translation="TLGIITEVTFKQLPMPGVRASVIGLFPELGQAARIVAKVLDSVL
                     LPEAMEFLNPEAVRLLAPALGLEGPTGYGLAVALAGTTETVERQMRDFVAIFQEGQAD
                     RTTTLHGGQTVPAWQAIRDVFDLPSGPPTERMICKIAVPISRTAETLAAAEELGRHHN
                     LRAVVTGHAGSGIVVACYLLPKGGPPAEALADSLEEFRREVEAREGSLVLQTAPQAIK
                     TLVDAWGKPGEAFDVMRRLKADFDPRGLCNPGRFLGGI"
BASE COUNT          508 a         1017 c          905 g          514 t
ORIGIN      
        1 agccaacgcg tcaccggtta tcctttcgat ccgcaaggga ccaggcgtga gcacattttc
       61 agacgtcccg gcggaagaac atccccgcca tccaaggacc ggatgtcctc cagccgcttc
      121 cgggcgctac gcggaaaggc ctgcccttcg gactccaggt tccgcggctg aacacccgca
      181 tcctgaaact cctctccgac cggcggatgc ccgagactcc aaacagccaa gagaccagac
      241 cgccctggag ctttagataa tccagggcct ggtggataat ccggtccagg atgctccggc
      301 cgtccgaacc ttccttgctc gccagcgagc agcaggggga ggttgttgaa aacctaacaa
      361 aggaaggatg aggcgtcaat cgcaccgcct ggccgttagc caggactgca acagcgaggt
      421 cggctgaaag ggaaaggtgg cgaggtcatg ctgaggccat gccttgttgc gatcatgctg
      481 ggcctgctca tgctggccgg cttccccgcc gaggcggcca agtggagccg cgcctacatc
      541 cgacagcttc ccgactcggc ctttgcggcc atcgagacta cgcccgacgg caagacgctg
      601 cggcatcttc cccaccacga cgccgcgggg aatctggacc tgcctcatct ccggagcgcg
      661 ctgtcccggc tcagccaggt caagtggcag aatcctgcca ccgctgggtc cgcccgccag
      721 catctcctgg atcatatgcg ccagcaccag caggaacgcg gcaagtctcc tctcaaggga
      781 tcgccctgac ccccaccccc tttatcctcc actccggaaa agacgcggcc cgcatccacg
      841 acgagggtgc gggccggatc aagtggcgag gtatggtctc aggctttgcc ggcggcgtag
      901 gcctgatcga gaagctggct cacgtggcgc acctgcaccg acaggccgcg acgcttcaca
      961 ccgtaggcca gatgcatcat gcacgcgggg cactccgtgg ccaggattgt ggccccggtc
     1021 ttctccacgt tccccatctt ccgatccagc actcccatcg cctccgcatg gtgaaggaag
     1081 gtataggttc ccgccgcccc gcagcaccag tcggcctcgg gaagttcttt gtaggtcgca
     1141 cccgggatcg acttcagcag ttttcgtggc tgcgcggtga ctttggcgaa gcgattggac
     1201 aggtggcagg ggtcgtggta ggtgaccgtc ccctgcagcg ggccaagact cccgtttgcg
     1261 cctgccgcca ccaggaactc gctgaagctc tgaatcttct tcgacagggc ggtggccttc
     1321 acggcatagg ccggatcccc tttcagcaga ctcccgtaat ccttcaggtg tgtgctgcag
     1381 ctcccgcatt ctgacacgat tacgtccaga cccatggccg gcgcgagacg atccacgttt
     1441 cttctggcaa tgtcgcgagc cgcctcgaga tccccgtagg attgggccgg ccggccacag
     1501 caggtgttgt ccagcacggt gatggcgcag ccgctctgcg ccaggacgcg tagcgtagcg
     1561 tcgacgacct cggggaactg gaagctgaac ccgcaggaga caaagtatcc gatccggtgc
     1621 ttcaccgtcg cgggggtggg cacctgtgcg ccccctccgc gcaggaaggg tttcgccgtc
     1681 agcgggggga ccacttggct ggccaccgcc agccgtttgt ctacgagatt caggataccg
     1741 gtcttctccg ccagcgtcgc caggccgttc cgttttgccc agagagccat gcgtccggcc
     1801 gtctctaacc tcttgctgtt cgagagcacc gatcggaaca ggacgcgctg ccacatcggc
     1861 tggccgtggc gggcgatgtt ggcctgccgg atcgccgtca caatctcgtc cgtcttgagc
     1921 gctgggaagc agtgggccgt gcatccgcga caaagcaggc agtcgtccag cgcgtgagcc
     1981 acctccggag tcaactcaag ctgccccagg atcagcgcgc gtgccaccgc catgcgtccc
     2041 cgggccagcg agtgctcctc tccggtcact cggaacaccg ggcagccggc ctggcagaat
     2101 ccgcagcgat tgcactgcgc tatcacatcg tacaggggga ggagttccga tgtcgccatg
     2161 gcccgccctc gcctcagatc ccgccgagga atctccccgg gttgcagagg ccccgaggat
     2221 cgaagtccgc cttcagccgc cgcatcacat cgaaggcctc gcccggcttc ccccaggcat
     2281 ccacgagcgt cttgatggcc tggggggccg tctgcaaaac caggctaccc tctcttgcct
     2341 ccacctcgcg tcgaaattcc tcaaggctgt cggccagggc ttccgccggg ggcccccctt
     2401 tcgggagcag gtagcacgcc accacgatgc cgctgccggc atggcccgtc accacagccc
     2461 ggaggttgtg gtggcggccc agctcttctg ccgccgccaa ggtctctgcc gttcggctga
     2521 tgggtacggc gatcttgcag atcatccgct ccgtcggtgg accggacggg aggtcgaaca
     2581 catcgcggat ggcctgccac gcgggaaccg tctggccgcc gtggagggtt gtcgtcctgt
     2641 ccgcctgtcc ctcctggaag atcgcgacga aatcccgcat ctggcgctcc accgtctcgg
     2701 ttgttcccgc caaggccact gccaagccgt aaccggtcgg gccttccagg ccgagggccg
     2761 gcgcgagaag tctgacggcc tccgggttca ggaactccat cgcctcgggg agcaggaccg
     2821 aatccaggac cttcgccacg atcctggctg cctgaccgag ttcagggaac agcccgatca
     2881 cgctggcccg aaccccgggc atggggagct gcttgaacgt gacctcggtg atgatcccca
     2941 gggt
//
LOCUS       DBNGZC010000674         1692 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2501503, whole genome shotgun sequence.
ACCESSION   DBNGZC010000674 DBNGZC010000000
VERSION     DBNGZC010000674.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2501503"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="AC16G0_11695"
     CDS             <1..678
                     /locus_tag="AC16G0_11695"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5100310.1"
                     /translation="TSPYVAQLANFFTRYHEDPPRLDVIRAGLETAVKTDPDVPNLLA
                     LAQVWFIWGDIRATTEDQKLQAYDRGRQAAERVVELEPKNVLAHLWYAINSARWGQTK
                     GVVRSLFLLPTVKREINTILELDPKLPPAYSLAGNVYYEVPGPLGGDLATAERMFRKG
                     LELDPRFTSLRVGLAKVLIKQGRLAEARQELQRVLDEKHPESPADWTMKDSTRARALL
                     DSLDKPS"
     gene            complement(908..>1692)
                     /locus_tag="AC16G0_11700"
     CDS             complement(908..>1692)
                     /locus_tag="AC16G0_11700"
                     /inference="COORDINATES: protein motif:HMM:NF025019.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5100311.1"
                     /translation="DVLHAIVRRRALSDDLIRTAWLAGGFPEPALLEDTAARRRWFDA
                     YVRLVSERDLGELQPDLRPPLVRRLLRMLAARQGQTVNLASLATEFGVPVVKVRGFLE
                     LLEGAFLWRRVEPYSANIGKRLTRSPRGWIADSGLLHALLDLRGPEDLAVHPLVGASW
                     EGWVQAQLLAQASLLDVPPAFAYWRTHAGAEVDIVLESGNRLIPIEVKRATRIGPYEL
                     RGLTSFLEAFRKRAAFGVVLYNGEDALRASERIALVPIARIL"
BASE COUNT          323 a          551 c          546 g          272 t
ORIGIN      
        1 acatcgccgt acgtggcgca gcttgccaac ttcttcaccc gctaccacga ggatccgccc
       61 cggctggacg tgattcgggc cggtctggag acggcagtca agacggaccc ggacgtgccg
      121 aatctcctgg cgctggccca ggtctggttc atctggggag acatccgggc gacgacggag
      181 gaccagaagc tccaggcgta cgatcgcggg cggcaagcgg ctgagcgagt agtggagctg
      241 gagccaaaga acgtcctggc ccacctctgg tatgcgatca actctgcccg ctggggccag
      301 accaaggggg tggtgcgatc gctgttcctc ctgccgacgg tcaagcggga gatcaacacg
      361 atcctggagc ttgaccccaa gctgccgcct gcatactcgc tggccggtaa cgtgtattat
      421 gaggttccgg gtccactggg cggcgacctg gcaacggcgg agcggatgtt ccggaaaggc
      481 ctggaactgg acccgcggtt cacctccttg cgcgtgggcc tggcgaaggt gctgatcaag
      541 caagggcgcc tcgccgaggc acgccaggag ctgcagcgcg tcctggacga gaagcatccc
      601 gagagcccgg ccgactggac catgaaggac agcacgcgcg cccgtgccct cctggatagc
      661 ctggacaagc cgtcctgacc gcaatcagag aatccaaccg caagacgccg ggaacaccag
      721 cgccgggtgc cgggacgccc tgtcaggcca ctcggtgctt cacaataagc tcgcagatcg
      781 cggcagccaa ttcgcgcgga accacatcac cgtctgcgtc gggtcctcga aggccgcttc
      841 gtcctcatga tccgggggtt gtccggctgt gggaggccgt acagaaacag ggggtcaggt
      901 gtcagtgtca cagaattcgc gcgatcggca cgagtgcgat gcgttcggag gcccggagcg
      961 cgtcttcgcc attgtagagg accaccccga aggctgcacg cttgcgaaaa gcctcgagga
     1021 atgacgtgag cccccgcaac tcgtaaggtc cgatgcgggt ggcgcgtttc acctcgattg
     1081 gaatcaaccg attccctgac tccaggacga tgtcaacctc ggcccccgca tgggtcctcc
     1141 aatatgcgaa ggccggtggt acatccagca gcgatgcctg ggcgagaagc tgcgcctgta
     1201 cccatccctc ccagcttgcc cccaccagag gatgcacggc gagatcctct ggcccccgaa
     1261 ggtccagcag cgcatgcagc agtccggagt cggcaatcca cccccgcggc gaacgggtga
     1321 gccgcttgcc aatgttggcc gagtagggtt ctacccgacg ccagagaaat gctccctcca
     1381 ggagttccag gaaacctcgc accttgacca ccgggacgcc gaattcggtg gccagggatg
     1441 ccaggttcac cgtttgaccc tgccgtgccg cgagcatgcg gaggagccgc ctcacgagcg
     1501 gtggacgcaa gtcgggttgt agttccccca gatctcgttc gctcactagc cggacgtagg
     1561 catcaaacca ccggcgcctg gcagccgtgt cctccaggag ggccggttca gggaatcctc
     1621 ccgccagcca ggcggtgcgg attagatcgt ccgaaagtgc ccgccgcctc acgatggcgt
     1681 ggagcacatc gt
//
LOCUS       DBNGZC010000675         2171 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2309845, whole genome shotgun sequence.
ACCESSION   DBNGZC010000675 DBNGZC010000000
VERSION     DBNGZC010000675.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2309845"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1614
                     /locus_tag="AC16G0_11705"
     CDS             67..1614
                     /locus_tag="AC16G0_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5100312.1"
                     /translation="MNRRKTSRREFLRLAAVGAGGALVAGRSVARAATSSAKALTVVQ
                     TAWPQSLDPSMDTNVNSGNMYAHIFESPAQYRYDEREKVMKLEARLCDKWENRAPDRW
                     RFHLRPGLKFTNGEEINSEVAKFSMETIKGNKGMAAPFMAHIKEVVPVDKLSFDVVTE
                     GPYAATPASTAFFYFFPPKYYVESGGKTRFGRKPIGSGPYKFVEWQEGVHVKLEANPA
                     YWGPKPQIPAITFRAQPETATRVALLDTGEADLITALSPELIDRVKRTADIRTVRGLR
                     RVFCFFNTNVAPTDNPLVRKAINYAVDVEAIVKNVLEGHAYVAKGINKPGYVGYAPDK
                     ITGYKYDPEQAKKLLAQAGHPNGIDLDYNHPIGRWLNDKDVAAAIIDMAAKVGIRFRD
                     KGVEFNTVSSLYTTQKATGMNMWSVGPLWLDPSYEWQVHFWTRGLYHYTNDAKMDGLL
                     DRLRMEVDPAKRVPLAQEFEKYVVDEYCPWLFLYDEEQIYGANKKLHWTPSPFERMDL
                     QYATMAA"
     gene            1660..>2171
                     /locus_tag="AC16G0_11710"
     CDS             1660..>2171
                     /locus_tag="AC16G0_11710"
                     /inference="COORDINATES: protein motif:HMM:NF040248.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5100313.1"
                     /translation="MWRYIRRRLVLAVLTYWAVSTVVFGLLYLTGDPVAILLAGTPAG
                     QVQLEELRHELGFDRPVPIQYLRFLASAVQGDLGKSLRSGEPALLLVSRRVGFTVQLA
                     FLAMVMALLVSIPIGIVSAMLRGSLLDRMLMLLSMLGQSMPIFWVGLLMIAVFAVGLR
                     WFPAGGAGGI"
BASE COUNT          403 a          736 c          664 g          368 t
ORIGIN      
        1 gaaccgccgc cgttccatcc aatccgatca cgcatcagcc gcggacgcac gcggaagggg
       61 gagcccatga atcgccgaaa aacaagtcgc cgtgagttcc tgcgtcttgc cgccgtggga
      121 gcaggtgggg cgttggtggc gggtcgctct gtggcccggg ctgcgacgag ctccgccaag
      181 gccttgaccg ttgtccagac agcctggccc cagtccctcg atccctccat ggacaccaac
      241 gtcaactccg gcaacatgta cgcccatatc ttcgagagcc cggcgcagta ccggtacgat
      301 gaacgcgaga aggtcatgaa gctcgaagcg cgtctctgtg acaagtggga gaaccgagcc
      361 cccgaccgct ggcgattcca cctccgcccg ggcctcaagt tcacgaacgg agaggagatc
      421 aactcggagg tcgccaagtt cagcatggag accatcaagg gcaacaaggg catggcggcc
      481 cccttcatgg cccacatcaa agaggtggtc ccggtggaca agctcagctt cgacgtggtg
      541 accgagggcc cctacgcggc caccccggcc tctaccgcgt tcttctactt cttcccgccc
      601 aaatactacg tcgagtcggg cggcaagact cgcttcggcc ggaagccgat cggcagcggc
      661 ccctacaagt tcgtggagtg gcaggaaggg gtgcacgtca agcttgaggc caaccccgcc
      721 tactggggcc ccaagccaca gatccccgcc atcaccttcc gggcccagcc cgagaccgcc
      781 acccgcgtgg cactgctgga taccggggaa gccgatctca tcaccgccct ctcccccgaa
      841 ctgatcgacc gggtaaagcg gacggccgac atccggactg tccgcgggct gcggcgggtg
      901 ttctgcttct tcaacaccaa cgtcgccccc accgacaacc ccctggtgcg gaaggccatc
      961 aactacgccg tggacgtaga ggcaattgtg aagaacgtcc tggagggaca cgcctacgtg
     1021 gcgaagggga tcaacaagcc gggctacgtc ggctacgccc cggacaagat cacaggctac
     1081 aagtacgatc cggagcaggc caagaagctc ctggcgcagg ccggacatcc caacgggatc
     1141 gacctggact acaaccaccc catcggccgc tggctgaacg acaaggacgt ggccgcggcc
     1201 atcatcgata tggccgccaa agtgggcatc cggttcagag acaagggggt ggagttcaat
     1261 accgtgtcct ctctctacac cacccagaag gccaccggta tgaacatgtg gtcggtgggt
     1321 cccctctggc tcgacccctc ctacgagtgg caagtccact tctggactcg cggcctatac
     1381 cactatacga atgacgccaa gatggatggg ctcttggatc ggctgcggat ggaggtcgat
     1441 ccggccaagc gcgtcccgct ggcgcaggaa ttcgagaagt acgtcgtgga cgagtattgc
     1501 ccctggctgt tcctgtacga cgaggagcag atctacggcg cgaacaagaa gctccactgg
     1561 accccgagcc ccttcgagcg gatggacctg cagtacgcga ccatggccgc ctaagcctct
     1621 ggcgcagaga gccaccgccg gagccaagga agcctgtgaa tgtggcggta catccgtcgc
     1681 cgcctggtgc tcgctgtcct cacgtactgg gcggtgagca ccgtcgtctt cggcctgcta
     1741 tacctgaccg gcgaccctgt cgccatcctg ctggccggaa ccccggccgg ccaggtccag
     1801 ctcgaggagc tgcggcacga gctgggcttc gaccggccgg tgccgatcca gtacctccgc
     1861 tttctggcca gcgccgtcca gggtgatctg gggaaatccc tgcgctccgg cgagccggcc
     1921 ctgctcctcg tctcgcggcg tgtcggcttc acagtgcagc tcgctttcct ggccatggtc
     1981 atggccctcc tggtctccat tcccatcggc atcgtctcgg ccatgctccg aggctccctc
     2041 ctggaccgca tgctcatgct gctcagcatg ctgggtcagt ccatgccgat cttctgggta
     2101 ggcctgctca tgatcgccgt gtttgctgtc gggctgcgct ggttcccggc cggcggcgcg
     2161 ggagggatca g
//
LOCUS       DBNGZC010000676         2339 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1034066, whole genome shotgun sequence.
ACCESSION   DBNGZC010000676 DBNGZC010000000
VERSION     DBNGZC010000676.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1034066"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="AC16G0_11715"
     CDS             complement(<1..1126)
                     /locus_tag="AC16G0_11715"
                     /inference="COORDINATES: protein motif:HMM:NF013206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR5100314.1"
                     /translation="MAKFTTRPVIMGRRGVVTSGHYLATAAGFRIMEQGGNAIDAAAA
                     MCFCLNLLEPQSNGIGGEVPTLIYSAPHRRVFAVCGMGWSATAFSLDWCRQHGIDLIP
                     GDGYLPACVPSMVDTWATAVARFGSLSFSQVLRPAIELAENGFAVYPGLHESLAGNLT
                     KFTETYPTTGEIYCPGGRVPEVGELLRNPDWASTLRLMCRAEAAGKQQGRVAGIEAAR
                     DAFYKGEIAERIVAFITTSPVEDASGTPHTGLLSYEDMAEWHAAVEEPVASPYGDLDV
                     HKCPPWTQGPVFLQQLAILEGYDLAALGHGSAEYLHLLIESAKLAFADREAYYGDPLF
                     DKVPLDILLSKEYTAKRRSLIGADASREMRPGDVGQGIPAY"
     gene            complement(1307..1834)
                     /locus_tag="AC16G0_11720"
     CDS             complement(1307..1834)
                     /locus_tag="AC16G0_11720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100315.1"
                     /translation="MLLRSMLVAVLVVEVVGCASLRSPGMGGGQSPASPDAFAHRTAS
                     SHVVLYWNCARPAPTVLRVEGIAQNPWESQEVRSLEFELVGVDAGERAVSEVTGEARE
                     PAIGTNQTSPFLLELRIVGSEARFDLYYDYQFSEGDQTDALLAGPAMLGLRLPPQGNR
                     FLVRDVCAESQHRSR"
     gene            complement(1958..>2339)
                     /locus_tag="AC16G0_11725"
     CDS             complement(1958..>2339)
                     /locus_tag="AC16G0_11725"
                     /inference="COORDINATES: protein motif:HMM:NF014017.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HIR5100316.1"
                     /translation="ARLRLFLEYLLRQRVLPGVRAAFEFREAGWLTPEVFTALEKANV
                     ALCLADWPDLVVEGPVTADFVYIRRHGATGRYVGSYSPAALRHQADRIRGWLAEDRSV
                     YVYFNNDAEAHAVANALRLKELLA"
BASE COUNT          426 a          817 c          727 g          369 t
ORIGIN      
        1 cataggcggg aataccctgc ccgacatcgc cgggccgcat ctcccgcgac gcgtccgccc
       61 cgatgaggct cctccgtttc gccgtgtatt ccttcgagag caagatatca agggggacct
      121 tgtcgaagag cggatcaccg tagtatgcct cgcggtcggc gaaggccagc ttggcggact
      181 cgatcagaag gtggagatac tccgcggagc cgtgtcccaa ggctgccaga tcgtaaccct
      241 ccaggatcgc caactgctgc aggaagaccg gcccttgcgt ccagggcggg cacttgtgga
      301 cgtcaaggtc tccgtacggc gacgccaccg gctcttccac cgccgcatgc cattccgcca
      361 tgtcctcata cgagagcagg ccggtgtggg gagtgccgct agcgtcttcc accgggctgg
      421 tcgtgatgaa cgccacgatg cgttcggcga tctccccctt gtagaaggca tcccgcgccg
      481 cctcgatgcc ggcgacgcga ccttgttgct tgcctgccgc ctcggcccgg cacatgagcc
      541 tgagggtact cgcccaatcc ggattccgaa gcagctcgcc gacctcgggc acacgccctc
      601 ccggacaata gatctcgccc gtggtcggat aagtctcggt gaacttcgtg aggtttcccg
      661 ccaggctctc gtgcaggccg gggtagactg cgaagccgtt ctccgccagc tcgattgcgg
      721 gccggaggac ctgggagaag gacaggctgc cgaaacgggc aaccgcagtg gcccaggtgt
      781 ccaccatcga cggcacgcac gccgggagat atccgtcgcc ggggatgagg tcgatgccat
      841 gctggcggca ccagtccagg gagaacgccg tcgccgacca ccccatcccg cagacggcga
      901 agaccctgcg gtgaggtgcc gagtaaatca gcgtcggcac ctcgccgccg atgccgttac
      961 tctggggctc caagaggttg agacagaagc acatggccgc ggcggcatcg attgcgttgc
     1021 cgccctgttc catgatgcga aaaccggcgg ccgtcgccag atagtgtccc gaggtgacga
     1081 cgccgcgccg tcccatgatc acgggtcggg tggtaaactt tgccatgccc atctccctct
     1141 gccggggtgc cgaccgggcc tctcgggacc acaagggccc tcccgtcagc gctccctctc
     1201 ggacgtgtcc accggccgcc atcccgcacg cgccccggag gtcgcggggc gccagtgccg
     1261 cggatactac ccccacgacc tgcccccgtc aatcccggac gggctgtcag cggctccgat
     1321 gttgcgattc cgcgcagaca tcccggacta ggaatcggtt cccctgtgga gggagccgca
     1381 atccaagcat tgccggcccc gccaacaagg catctgtctg gtcgccctcg gagaattggt
     1441 aatcgtaata caggtcgaat cgcgcctcgc tgccgacgat gcgcaactcc aggaggaaag
     1501 gcgaggtctg gttcgttccg atcgcgggtt ctcgcgcctc gcccgtgact tcggagacgg
     1561 cacgctcgcc tgcatccact cccacgagct caaactccaa agaccggact tcttgcgact
     1621 cccagggatt ctgggcgatg ccttccaccc gaaggacggt gggcgcaggc ctggcacagt
     1681 tccagtacag gaccacatgg gatgaagcag tccggtgggc gaaggcatcg ggggaggctg
     1741 gagactgtcc gccccccatc cccggcgacc ggagggatgc gcatcccacc acctccacca
     1801 cgagaaccgc gaccagcatg gaacgcagca gcataatctc cctcccgcaa gaagccttgg
     1861 ccgtacggaa cccccgcgat cacccgcggt cctctgttcg catgggaggc ctttcgggct
     1921 gccatccgcc accggccgcc cgggcacccc ggaaccctca cgccagaagt tccttcaggc
     1981 gaagcgcatt ggcgacggcg tgcgcctcgg catcgttgtt gaagtagacg tacacacttc
     2041 gatcctcggc gagccagccc ctgatacggt cggcctggtg acgcagggcc gcaggcgagt
     2101 aggaaccgac ataccggccg gtcgcgccgt gccgccggat atacacaaaa tcggccgtga
     2161 ccggtccctc gaccacgagg tccggccaat ccgccaggca caacgccacg ttcgccttct
     2221 caagggcggt gaagacttct ggggtcaacc aaccggcctc ccgaaactcg aaggccgccc
     2281 gcacgccggg aaggaccctc tggcggagca ggtactccag aaagagacgg agacgggcc
//
LOCUS       DBNGZC010000677         1719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1920035, whole genome shotgun sequence.
ACCESSION   DBNGZC010000677 DBNGZC010000000
VERSION     DBNGZC010000677.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1920035"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="AC16G0_11730"
     CDS             <1..348
                     /locus_tag="AC16G0_11730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100317.1"
                     /translation="LPLFLFFASGIFHVRSRVRVWLARRKGGASASSALVPCLVYHVL
                     LILVIPLLAILQILPWLALLAFAPAVCRAAAGLCQDGLDLDLKRLRWSEVALSTVFVL
                     LLVSAFHLTSVAG"
     gene            475..>1719
                     /gene="der"
                     /locus_tag="AC16G0_11735"
     CDS             475..>1719
                     /gene="der"
                     /locus_tag="AC16G0_11735"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR5100318.1"
                     /translation="MSVPVIAIVGRPNVGKSTLFNRLLGGRRAIVDDMPGVTRDRHYG
                     ECEWRARRFVLVDTGGFEPAGTGIMALVRAQAQQAVAEADVILLLVDAKDGLTPADAE
                     VARLLRTGSPRPILLVVNKVDTAGQEPMAAEFHRLGLNQVLPISAEQGQGIGELLDAV
                     VSILPAAENPPQATNAITVAVVGRPNVGKSSLVNRILGAERVLVSPEPGTTRDAVDTR
                     FTYRGHDYICVDTAGIRAKGRIGHSIERFSVSRALAAVRRADVALILLDGVEGVTSQD
                     TKVGGEAHEAGCASILVVNKWDCRAGDREAMAAFRLALQEKFKYLAYAPTAFVSAKTG
                     LHVMSLFRLIDAVAAERERRIPTSDLNAAIGEAVTRRPPPAERGRPVRIRYATQTGIK
                     PPTFLCFATAAGGLHFTYLRYLE"
BASE COUNT          249 a          581 c          566 g          323 t
ORIGIN      
        1 ctgccactgt tcctcttctt tgccagtggg atcttccacg tccggtcccg cgtgcgggta
       61 tggctcgcgc gccggaaggg gggagcatca gccagttcag ctctcgttcc atgtctcgtg
      121 taccatgtcc tgctcatcct ggtgattccc ctgctcgcga tcctgcaaat cctcccgtgg
      181 ctcgctctcc tcgcctttgc gcctgctgtc tgtcgcgccg ccgctggcct ttgtcaggac
      241 gggctggacc tggatctcaa gcgtttacgc tggtccgaag tggccctcag caccgtcttc
      301 gtcctcctct tggtcagcgc cttccacctc acatccgtgg cgggctgaac ctcacttctc
      361 agcctcccgc cgacttgcca cgaagccgcg acgcggcacc tcgtctggcg acaccactcc
      421 tcgggtggca gcccccctga ttctgtgata caagtccccc ggaggtatcg actcgtgtcc
      481 gtgcctgtca tcgccattgt cggccgaccg aatgtgggga aatccacgtt gttcaaccgc
      541 ctgctgggcg ggcgacgcgc catcgtggac gatatgcccg gcgtgacccg tgatcgccat
      601 tacggcgagt gcgagtggcg cgcccggcgc ttcgtcctcg tggataccgg tggtttcgaa
      661 cccgccggga ccgggatcat ggcgctggtg cgagcccagg cgcagcaggc cgtggccgag
      721 gcggatgtga tcctcctcct cgtggatgcc aaggacggcc tcaccccggc cgatgccgag
      781 gtcgcgcggc tgctccggac cggctcgccc cggcccatcc tgctggtggt caacaaggtg
      841 gacacggccg gccaggagcc gatggcggcg gagttccatc ggctggggct gaatcaggtc
      901 ttgcccatct cggccgagca aggacagggg atcggcgagc tgctggatgc ggtggtttcg
      961 attctcccgg ccgcggagaa ccctccgcaa gcaaccaatg cgatcacggt ggcggtggtg
     1021 ggacgcccca acgtcgggaa atcctcactg gtcaaccgga tcctgggtgc cgagcgagtc
     1081 ctggtgagtc cggagccggg gacgacccgg gatgccgtgg acacgcgatt cacgtaccga
     1141 ggtcacgatt acatctgtgt cgatacggct ggcattcgag ccaaggggcg gatcggccac
     1201 tcgatcgagc ggttcagcgt ctcccgcgcc ctggcggccg tgcggcgcgc cgacgtcgcc
     1261 ctgatcctgc tggacggggt cgagggggtc acgagccagg acaccaaggt cggcggggag
     1321 gcccacgagg cgggttgcgc cagcatcctg gtcgtcaaca agtgggattg ccgtgcgggt
     1381 gaccgcgagg ccatggccgc ctttcgcctt gcgctgcagg agaagtttaa gtatcttgcc
     1441 tacgccccga ccgcgttcgt ctccgccaag accgggctcc acgtgatgtc gctgtttcgc
     1501 ctgatcgacg ccgtggcggc cgagcgcgaa cggcgcatcc cgacctcgga cttgaatgcc
     1561 gccatcgggg aggcggtaac gcggcggccg ccacctgcag agcgagggcg tccggtgcgc
     1621 atccgctacg cgacgcagac gggcataaag ccgccgacgt tcctctgctt cgccacggct
     1681 gctgggggat tgcacttcac ctatctgcgc tacctggag
//
LOCUS       DBNGZC010000678         1870 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2407000, whole genome shotgun sequence.
ACCESSION   DBNGZC010000678 DBNGZC010000000
VERSION     DBNGZC010000678.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2407000"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="AC16G0_11740"
     CDS             <1..626
                     /locus_tag="AC16G0_11740"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR5100319.1"
                     /translation="FGQNVATVRKALGRQLAREAPAEADLVIPVPDSGVPAALGFAQE
                     SGLPFDHGLIRNHYVGRTFIEPKQNIRHFGVKIKLNPVREILEGKRVVVVDDSIVRGT
                     TSRKIVSMVRSAGAREVHMRISSPPTVSPCYYGIDTPTRKELIASSHTVNETCRYIRA
                     DSLGYLSIAGLLKAVGKDNGFCHACFSMQYPVAFPTEDRAQLNLFEP"
     gene            651..923
                     /locus_tag="AC16G0_11745"
     CDS             651..923
                     /locus_tag="AC16G0_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100320.1"
                     /translation="MVADHTDPHERARSLARLIVGEIALYNKEKIAEGIRNDTLFEVL
                     ERELDVGRRYYQKNVEPEVEAQADYFNEAVVDILVKGGADLESKIW"
     gene            complement(1234..>1870)
                     /locus_tag="AC16G0_11750"
     CDS             complement(1234..>1870)
                     /locus_tag="AC16G0_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR5100321.1"
                     /translation="TYIRPIAYISSEGIGPKLVGYDTGFGIYTLPLGDYIDTSAGIKV
                     GVASWRRINDNTIPARCKVTGSYVNSALAKTEALEQGFDEAIFLTQDGYVCEGSAENI
                     FLVRDGVLVTPDLSQDILEGITRNVLISLCRDELGLKVVERQVGRTELYIAEEAFLCG
                     TGAQVSSIVEVDRRPVGNGRIGPITTRIQQLYFEVVKGRHPKYRHWCTPVY"
BASE COUNT          359 a          568 c          607 g          336 t
ORIGIN      
        1 tgttcggcca gaacgtggcc acggtgcgga aggccctggg ccggcagctg gcccgggagg
       61 ccccggccga ggcagacctg gtgatccccg tgcccgactc gggggtgccg gccgccctgg
      121 gcttcgccca ggagtcgggg ctgccctttg atcacggcct catccgcaat cactatgtgg
      181 ggcggacctt catcgagccc aagcagaaca tccgtcactt cggggtcaag atcaagctga
      241 acccggtgcg cgagatcctg gagggcaaac gggtcgtggt ggtggacgac tcgatcgtgc
      301 ggggaacgac cagccggaag atcgtatcca tggtgcgatc cgccggggcg cgagaggtcc
      361 acatgcggat cagctcaccc cccacggtgt ctccctgcta ctacgggatc gacacaccca
      421 cgcggaagga gttgatcgct tccagccaca ccgtgaatga gacctgccga tacattcggg
      481 ccgattccct cggctatctc agcatcgcgg gtctcttgaa ggcggtgggc aaggacaacg
      541 ggttctgcca tgcctgcttc agcatgcaat atcccgtggc gttcccgaca gaggatcggg
      601 cccagctcaa cttgttcgag ccctgagccg ttctcccgag gaggacaggc atggttgcgg
      661 atcacacgga cccacacgag cgcgccagga gcctggcgcg gctgatcgtg ggagagatcg
      721 ctctctacaa caaggaaaag atcgcggagg ggatccggaa cgatactctc ttcgaggttc
      781 tggagcgcga gctggacgtg ggccggaggt actaccagaa gaacgtggag cccgaggtgg
      841 aggcccaggc ggactacttc aacgaggcgg tggtggatat cctggtgaag ggaggggccg
      901 acttggaatc gaagatctgg tgatgttccg cctgaagtag ccggcggcag cctccaatta
      961 accggattat ctcaccgcga aattcgcaat gtgcgcaaag acagacgtag ctggaggatt
     1021 caaccccgga gtaaggaggc gaaatgttcc ggcgtcccag tgggcgctgc ctcaggatct
     1081 cctgagaccc aaaagcgttt tctctgtgtg cttggcgtgc tctgcggttg aactgcagta
     1141 ttcgggatta acacatcaac gcggctcgga agtcttccgg gccgcgccat gtttatccgc
     1201 cgtccaccgc cttcccccag cccctggaac cggtcagtag acgggcgtgc accagtggcg
     1261 atacttgggg tggcgaccct tgacgacctc gaagtacaac tgctggatac gtgtcgtgat
     1321 gggtccgatc cggccattgc ccacgggccg ccgatccacc tcgacgatgg aggagacctg
     1381 ggcaccggtg ccgcagagaa aggcctcctc ggcgatgtac aactcggtcc gacccacctg
     1441 gcgctcgacc accttgaggc ccaactcgtc ccggcatagg gagatcaaga cgttgcgcgt
     1501 gatcccctcc aggatgtcct gggacaggtc cggggtgacg aggaccccgt cgcggaccag
     1561 gaaaatgttc tcggccgagc cctcgcacac gtagccgtcc tgcgtgagga agatggcctc
     1621 atcgaagccc tgttccaggg cctcggtctt ggccagggcg gagttcacgt aacttccggt
     1681 caccttgcaa cgggccggga tggtgttgtc gttgatccgg cgccaggagg ccacccccac
     1741 cttgattccc gccgaggtgt cgatgtagtc gcctaacggc agtgtgtaga tgccgaagcc
     1801 cgtatcgtag ccgaccaact tcggaccgat cccctcagag ctgatgtagg cgatgggccg
     1861 gatgtacgtg
//
LOCUS       DBNGZC010000679         2884 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1622451, whole genome shotgun sequence.
ACCESSION   DBNGZC010000679 DBNGZC010000000
VERSION     DBNGZC010000679.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1622451"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1169
                     /locus_tag="AC16G0_11755"
     CDS             <1..1169
                     /locus_tag="AC16G0_11755"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HIR5100322.1"
                     /translation="NKTAVRAQIRVGLEEYRRNFGCDSPGLWIPECGYAAGLDQLLEE
                     SGVRYFFVEAHGLLSAEPRPRFGVHAPVYTPSGVAAFGRDPESSKAVWSSVEGYPGDF
                     DYREFYRDIGYDLDYETIKPYLPGGGIRSNTGIKYHRITGRTDHKEPYVRSRAMEKAA
                     LHAGDFMANRQRQIEHLANHMDRRPIVVAPYDAELFGHWWFEGPQWIDFLVRKVAFDQ
                     KTFRLVTPSEYLDMYPVNQVCAPSTSTWGYKGHNEVWVNGNNDWIYPHLHAAADQMEE
                     LARTFPQARGLQGQALNQAARELLLLQASDWAFMMQTGATADYARRRTRSHLARFLRL
                     AQQIQGGTIQEGWLAQLEEADRLSPALDYRVYNPLPEGKSVETDRRQPSSRGRA"
     gene            complement(1199..2146)
                     /locus_tag="AC16G0_11760"
     CDS             complement(1199..2146)
                     /locus_tag="AC16G0_11760"
                     /inference="COORDINATES: protein motif:HMM:NF013197.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005851 - eukaryotic translation
                     initiation factor 2B complex [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="eIF2B alpha/beta/delta subunit family protein"
                     /protein_id="HIR5100323.1"
                     /translation="MSTDWQFTLRRIKHDRRSGASVLLGHGIEAGRLFLNTSRRFSPR
                     RLEAALMRFSLGLVGAQPAMAPFLRLANELWLAWEACLAPSRAWDSLHAALVRYAEGT
                     DRGLRETVRRAGALVRPGALVLTYSSSTAVRLALWQAMADGRRFTVVCSESRPMGEGV
                     ALARRLTALGIPVHLTLDAALLEWVERADLILLGADAILPGGVVNKVGSGPLFQIARL
                     AGVPAYVLADSGKWLPEGLARFWRVRPEDPREITRLRHPDLTVHNRYFGMWPLHSIQG
                     LVWEGGIASPADVRKRIARISVSLALVGFLGGLDAPISR"
     gene            2280..>2884
                     /locus_tag="AC16G0_11765"
     CDS             2280..>2884
                     /locus_tag="AC16G0_11765"
                     /inference="COORDINATES: protein motif:HMM:NF013183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIR5100324.1"
                     /translation="MERKPIMHAIILAVGREILRGRVHDSNSWTLARRLTGMGCEVLR
                     IVSCDDDLVAIVREIRRAQEDGAGLLITTGGLGPTEDDLTLRALAEAVGRPLALDAEA
                     SRLVASRYAELQRIGAVEDASMTPTREKMAWIPSGSRPLPNPVGASPGVWLEDGGVIY
                     VALPGVPAEMAAILEASALPALAPRMGGAVFLERSLRTAAR"
BASE COUNT          521 a          961 c          914 g          488 t
ORIGIN      
        1 tcaacaagac ggctgtgcgt gcccagattc gggtcggtct ggaagaatac cgacggaact
       61 ttggctgcga ttctccgggg ttatggattc ccgaatgcgg ctacgctgcc gggctggatc
      121 agctcctcga ggaatccgga gtccgatatt tcttcgttga agctcacggt cttctcagcg
      181 cggagcctcg gccgcgcttc ggggttcacg cgccggtcta cactccctcg ggagtggccg
      241 ccttcggccg ggaccccgag tcctcgaagg ccgtctggag ctccgttgag gggtacccgg
      301 gagacttcga ctaccgggag ttctaccgag acatcggata tgacctggac tacgagacga
      361 tcaaacccta tctcccggga ggcgggatcc ggtcgaacac ggggatcaag taccaccgga
      421 tcacgggaag gacggatcac aaggagccct acgtccggtc tcgggccatg gaaaaggccg
      481 ccctgcacgc gggggatttc atggcgaatc gccagcggca gatcgaacac cttgcaaacc
      541 acatggatcg cagacccatc gtggtggcac cctacgatgc ggagttgttc ggccattggt
      601 ggtttgaggg gccccagtgg atcgactttc tcgtccggaa ggtggccttt gaccagaaga
      661 cgttccgcct ggtcaccccc tctgagtacc tggacatgta tccggtgaat caggtgtgcg
      721 ccccctccac ctccacctgg ggttacaagg ggcacaatga ggtctgggtg aacgggaaca
      781 acgattggat ctacccccat cttcatgcgg ccgcagatca gatggaggaa ctggcgagaa
      841 catttcccca ggcccggggt ctccaaggcc aggcactgaa tcaggcggcc cgagagctcc
      901 tcctgctgca ggcgagcgat tgggctttca tgatgcagac cggggcgacc gcggactacg
      961 ccagaagacg gacccgaagc cacctggcgc gatttctacg tctggcgcag cagatccagg
     1021 gagggacgat ccaggagggg tggctggccc aactggaaga ggctgaccgc ctgtctcccg
     1081 cgctggatta tcgggtctat aatcccttgc ctgagggtaa gtccgtagag accgatcgcc
     1141 gtcaaccttc ttcccggggg cgggcctgag cggccacgac ctcccacagg gagaggcctc
     1201 atctggatat cggtgcgtcc agccctccga ggaatccaac cagcgccagc gagacggaaa
     1261 tccttgcgat ccgtttcctg acatccgccg gtgaggcgat tccgccctcc cacacgagtc
     1321 cttgtatcga gtggagcggc cacatcccaa aatagcggtt atgcaccgtg aggtcggggt
     1381 ggcgtaggcg ggtaatctct ctggggtcct ctggccgaac ccgccagaaa cgcgccagac
     1441 cttccggcag ccacttgccg gaatccgcca gcacgtaggc cggaacacct gcgagccggg
     1501 cgatctgaaa gaggggaccg ctccccacct tgttcaccac gccccctggc aggattgcat
     1561 ccgcgccgag gaggatcaga tcggctcgct cgacccactc caggagggcg gcatccagtg
     1621 tgaggtggac cgggatcccc agggccgtaa ggcggcgcgc cagggcaacc ccctccccca
     1681 tcggacgcga ctcagagcag accaccgtga accgccttcc gtccgccatg gcttgccaga
     1741 gggcaagccg caccgccgtg ctactggagt atgtgaggac gagggctccg ggccggacca
     1801 aggcccccgc ccgccgcact gtctcccgga ggccgcgatc ggtcccctcg gcatagcgga
     1861 ccagggcagc gtgcaggctg tcccacgccc tcgagggcgc aagacaggct tcccacgcga
     1921 gccatagctc gttcgccagc ctgaggaatg gcgccatggc gggctgagcg cccaccagcc
     1981 ccaggctgaa tcgcatcaac gccgcctcca aacgccgcgg ggagaagcgc cggctggtat
     2041 tgagaaacag gcggccggct tcgatgccgt ggccgagcag cacgctcgcg cccgaacgcc
     2101 gatcgtgctt tatccgccga agggtgaatt gccaatcagt ggacattgtc cccggtatcc
     2161 ccaatgcctg ctgacgtgcg gccactcgcc aagccgccga ccggtcaatt cgtttcctcg
     2221 tccctcgttc gcaaatcccg cgatcggcag caaccgagat atgctatgct gcagacttga
     2281 tggaacgcaa accgatcatg cacgcgatca tcctggccgt ggggcgcgaa atcctgcggg
     2341 gtcgagtcca cgattccaac tcctggacgc tggcccggcg gctcactggc atgggctgcg
     2401 aggttctgcg gattgtttcc tgcgatgacg accttgtcgc catcgttcgc gagatccgcc
     2461 gcgcccaaga ggatggcgcc ggcctcctca tcaccaccgg cggcctggga cccaccgagg
     2521 atgacctgac cctccgcgcc ctggcggaag ctgtgggccg tcctctcgcc ctggatgcag
     2581 aggcatcccg gctcgtcgcc tcccgttacg ctgagctaca gcggattggt gccgtggagg
     2641 acgccagcat gacgcccacg cgggaaaaga tggcctggat cccgtcgggc tcccggccgc
     2701 tgcccaatcc tgtcggcgcc tcccctggcg tatggctgga agacggcggc gtgatctacg
     2761 tcgcgctccc gggcgtccct gccgagatgg cagccatcct ggaggcttcc gccctccccg
     2821 cactcgcccc tcggatgggc ggtgcggtct tcctggagcg gagtctacgg acggccgccc
     2881 ggga
//
LOCUS       DBNGZC010000680         1787 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2407129, whole genome shotgun sequence.
ACCESSION   DBNGZC010000680 DBNGZC010000000
VERSION     DBNGZC010000680.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2407129"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1116)
                     /gene="ilvC"
                     /locus_tag="AC16G0_11770"
     CDS             complement(118..1116)
                     /gene="ilvC"
                     /locus_tag="AC16G0_11770"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR5100325.1"
                     /translation="MARLYYDKDADLGLLKGKTIGVIGYGSQGHAHALNHRDSGLEVM
                     VGLYKGSKSWEKAKGDGLKVGTVQETAEASQVIMILLPDQTHKQVYEESIKKALTPGK
                     TLMFAHGFNIHFNQIVPPPDVDVSMIAPKAPGHMMRQVFTEGSGVPALVAVHQNPSGK
                     AKELALAYARGVGCTRAGVLETTFKEETETDLFGEQTVLCGGVSALIKAGFETLVEAG
                     YQPESAYFECMHEMKLIVDLMYQGGLAYMRYSVSDTAEYGDYSRGPRVISQVVKDEMK
                     RILGEIQNGQFAREWILENQAGRPGFLAMRGKEAEHPIERVGKELRGMMSWLKGRR"
     gene            complement(1130..1696)
                     /gene="ilvN"
                     /locus_tag="AC16G0_11775"
     CDS             complement(1130..1696)
                     /gene="ilvN"
                     /locus_tag="AC16G0_11775"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR5100326.1"
                     /translation="MTEPKHHRKHILSILVENHQGVLAKIAALFAAKGYNIDSLSVAE
                     TLDPTVSRITMSVHGDEWVIEQAAKQLHRLIDVIKVVDLTDEAHVGREMVFVRVNAEP
                     MNRAEILRIVEIFRGRIVDVTPLSYTLEVTGEEGKIGAILELLQPFGIQEVVRSGTLA
                     IARGSKIVSKREESRLLRVASRKQAVAE"
BASE COUNT          274 a          635 c          515 g          363 t
ORIGIN      
        1 cacgaggaac accagcaact tgacgcgggt cccccctcga ggcggtcccg gggggaggcc
       61 ctcctggggc catccgacag atttctcgcg tgaggcgctc agtccaactc ctccaggtca
      121 gcggcggccc ttgagccagc tcatcatccc gcggagctcc ttccccaccc tctcgatggg
      181 atgctcggcc tccttacccc tcatggcgag gaagccgggg cgacccgcct gattctccag
      241 gatccattcc cgggcgaact gcccgttctg gatctcgccc aggatcctct tcatctcgtc
      301 cttcacgacc tggctgatga cgcgtgggcc ccggctgtaa tccccgtact cggcggtgtc
      361 gctcacggag taccgcatgt acgccaggcc accttggtac atgaggtcta cgatcagctt
      421 catctcgtgc atgcactcaa agtaggccga ctccggttga tagcccgcct ccaccagggt
      481 ctcgaatccc gccttgatga gcgccgacac gccgccgcag agcaccgtct gttcaccgaa
      541 caggtcggtc tcggtctcct ctttgaaggt ggtttccagc accccggccc gcgtgcagcc
      601 caccccacgg gcgtaggcca gggccagctc cttggccttg cccgatggat tttggtgcac
      661 cgccaccaag gcgggcacgc ccgagccctc ggtgaagacc tgacgcatca tgtggcccgg
      721 agccttggga gcgatcatgg agacgtccac atccggcggg ggcacgatct ggttgaaatg
      781 gatgttgaag ccatgggcga acatcaaggt cttgcctggg gtcaaggcct tcttgatgga
      841 ttcctcatac acctgcttgt gggtctgatc ggggagcagg atcatgatga cctgggaggc
      901 ctccgccgtc tcctggaccg tcccgacctt gaggccgtcc cccttggcct tctcccagga
      961 cttgctaccc ttgtacaggc ccaccatgac ctccagcccg ctgtcccgat ggttcagggc
     1021 atgggcgtgg ccctggcttc cgtagccgat gacgccgatg gtctttccct tcagcagccc
     1081 aagatccgca tccttgtcat agtaaagtct ggccatgggt ctcctcgttt cactccgcca
     1141 ccgcttgttt tcgactcgcc acgcgcagca gccgactctc ttcccgcttg gagacgatct
     1201 tgctgccccg cgcgatggcc agggtgccac tccgcacgac ttcctggatc ccaaagggct
     1261 gcagcaattc caggatggcc ccaatcttcc cctcttcgcc ggtgacctcc agcgtgtagc
     1321 tcaggggggt gacgtccacg atccgtcctc ggaagatctc gacgatccgc aggatctcgg
     1381 cccggttcat cggttcggcg ttcacccgca cgaagaccat ctcgcggccg acgtgggcct
     1441 cgtcggtgag gtccacgacc ttgatcacgt cgatgaggcg gtggagctgc ttcgccgcct
     1501 gctcgatcac ccactcgtcc ccgtggacgg acatggtgat ccgggagacg gtggggtcca
     1561 gcgtctccgc caccgagagg ctgtcgatgt tatagccctt ggccgcgaac agcgccgcga
     1621 tcttggccag gaccccctgg tggttctcca ccaggatgga caagatatgc ttccgatggt
     1681 gcttgggttc cgtcatcctt gctcccctgc ctccctctgc gcgctcgccg cccccgcggg
     1741 gcatctccga tcctgtccgt gccgacgggc gctcccctcc ccggacc
//
LOCUS       DBNGZC010000681         1800 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_742146, whole genome shotgun sequence.
ACCESSION   DBNGZC010000681 DBNGZC010000000
VERSION     DBNGZC010000681.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1800)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1800
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_742146"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1400)
                     /locus_tag="AC16G0_11780"
     CDS             complement(<1..1400)
                     /locus_tag="AC16G0_11780"
                     /inference="COORDINATES: protein motif:HMM:NF014176.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR5100327.1"
                     /translation="MRAFLRDVWDLLRKHVRLLAAASIVLLVGIFGLNFAASRVAQRS
                     SFCATCHYMAPYVEQWKTSNHAGVDCVSCHPYRARAVAASTIRYVSGAYNPRPRAEVQ
                     DRSCLASGCHEQRLLKTQETFRGGIRFDHQVHLKTMPRGIQLRCTSCHNQIVQKGHVA
                     VTEQACYLCHFKGAGPGEAVTGCETCHGSPKKTVEHAGISFSHQSYLKVGVACSQCHV
                     RVVVGEGGVPKERCASCHIGREDRIQDVQFLHENHITRHNVDCQECHEPLRHGKIQMV
                     EPLEVRCESCHIRQHSLRKLMYIGTGGKLIPDLPSRMFAAQVSCTGCHIHVTEKGAVL
                     SHEARTTAQREACVTCHGQGFDKMYDDWKTLMAKLLQGYGAFLAEAEKQIAGKPLPKK
                     HATALQDAREAYLFVKEGRGEHNVEYAVKLAQAGVARVDALFQALDPKAKPIPRDDLL
                     GQKDAYCYPLCHQRLP"
     gene            complement(1438..>1800)
                     /locus_tag="AC16G0_11785"
     CDS             complement(1438..>1800)
                     /locus_tag="AC16G0_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100328.1"
                     /translation="GLILWFLETSLSYLPKFAADIAREAHSDEGLLATLAIIIWHFYN
                     VHLNPEHFPMNRTFWTGTISEEEMRRHHPLEYEALRASRATRLSATGATSAARGIVEN
                     PSLQPLKKVLNAPGNETS"
BASE COUNT          308 a          568 c          573 g          351 t
ORIGIN      
        1 aagggtaacc gctggtgaca cagcggatag cagtacgcgt ccttctggcc gaggaggtca
       61 tctcgaggaa tcggcttggc cttggggtcg agggcctgga agagcgcatc cacccgtgcc
      121 acgccagcct gggccagctt gaccgcgtat tccacgttgt gctcgcctcg gccttctttg
      181 acgaacagat aggcctccct ggcatcctgc agcgccgtgg cgtgcttctt ggggagaggc
      241 ttccccgcga tctgcttttc tgcctcggcc aggaaggcgc cgtagccttg gagcagcttc
      301 gccatgaggg ttttccagtc atcatacatc ttgtcgaagc cctgaccgtg gcaggtgacg
      361 cacgcttccc gctgggcggt cgttcgagcc tcgtgggaca gcaccgcacc cttctccgtc
      421 acatggatat gacagccggt gcagctcacc tgtgcggcga acatccggct gggcaaatca
      481 gggatgagct ttccgcccgt cccgatgtac atgagcttgc gtagactgtg ctggcggata
      541 tggcaactct cgcagcgaac ctccagcggc tcgaccatct ggatcttccc gtggcgcaac
      601 ggctcgtggc actcctggca atccacgttg tgccgggtga tgtgattctc gtgcaggaac
      661 tggacgtcct ggatgcggtc ctcacggccg atgtgacagg aggcgcaacg ctccttcgga
      721 actcccccct cacccacgac cacccggacg tggcactgac tgcaggcgac ccccaccttc
      781 agatacgact gatggctgaa ggagatcccg gcgtgttcca cggttttctt cgggctccca
      841 tggcaggtct cgcagccggt gaccgcctcg cccgggccgg cccctttgaa gtgacagagg
      901 tagcacgcct gttcggtcac cgccacgtgt cccttctgga cgatctgatt gtggcagctg
      961 gtgcagcgaa gctggattcc tcggggcatg gtcttgaggt gaacctggtg atcgaaccga
     1021 atccccccac gaaatgtctc ctgcgtcttg agcaggcgct gttcgtgaca cccgctggcc
     1081 aggcagctcc gatcctgcac ttcggcccgg gggcgaggat tgtaggcccc gcttacgtag
     1141 cggatcgtcg acgcggccac cgcacgcgcg cggtagggat gacagctcac gcaatccacg
     1201 ccggcgtggt tcgaggtctt ccactgctcc acgtagggcg ccatgtagtg acaggtggcg
     1261 cagaagctcg agcgctgggc gacccgcgag gcggcgaagt ttagtccgaa gatgcccacc
     1321 agcaatacga tgctggcggc ggccaggagt ctcacgtgct tgcgcaagag gtcccacaca
     1381 tcgcgaagga atgcccgcat atgctcctca agagatcagc cgcccgaggg cccagcctca
     1441 ggaggtctcg ttccctggcg cgttcaggac tttcttcagt ggctgcaacg agggattctc
     1501 cacaatgcct ctggccgcgg aagtcgcccc ggtagccgac agacgggtcg cacgggacgc
     1561 cctcaacgcc tcgtactcca gcgggtggtg ccggcgcatc tcttcctcgc tgatcgttcc
     1621 ggtccagaag gtccggttca tggggaagtg ctcgggattc aggtggacgt tgtagaaatg
     1681 ccagatgatg atggccagcg tggccagcag gccctcgtcg ctgtgtgcct cccgggcgat
     1741 gtcggcggcg aatttgggca ggtagctgag ggaggtctcg agaaaccaca ggatcaggcc
//
LOCUS       DBNGZC010000682         2087 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_157357, whole genome shotgun sequence.
ACCESSION   DBNGZC010000682 DBNGZC010000000
VERSION     DBNGZC010000682.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_157357"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="AC16G0_11790"
     CDS             complement(<1..474)
                     /locus_tag="AC16G0_11790"
                     /inference="COORDINATES: protein motif:HMM:NF022892.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2950 family protein"
                     /protein_id="HIR5100329.1"
                     /translation="MAGFLFLPAAFGQGKGNPQKSFATPEAATKALAKAYKKGDNKTV
                     AEILGDKAGRLVLSGDPVIDRHEREWFLSLYQEGHEVVAEDESRVVLQLGKNEQPYPI
                     PLIKKGAQWRFDSSEGHEDLVSRRISKTELTAVNLVSAYVDAQRAYHETPYRNDGV"
     gene            complement(949..1341)
                     /locus_tag="AC16G0_11795"
     CDS             complement(949..1341)
                     /locus_tag="AC16G0_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100330.1"
                     /translation="MASRISWFGFGVGALVGGVIRVAVAVLHIPSITGEQYAVILLPA
                     VLGIVIGGISGGTGRILLGAVVGAGLSILFYLGSVPLVGLLAFLGAATLPSLWEVLAV
                     GAVPGAIGGAVGQFVTKRRGGAPAPRSS"
     gene            complement(1509..>2087)
                     /locus_tag="AC16G0_11800"
     CDS             complement(1509..>2087)
                     /locus_tag="AC16G0_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100331.1"
                     /translation="LEGRSNTRRLWPTARLLACWLAVLVTLAGCASLAVTRCDSVPDR
                     DGKYFGRFFLGVATLGLSEVTIQQEEVRQAYEGSPGCPPPSGVRVVSAQKPSSAGGQS
                     AALGTLVNGTRWTIGVYLNQDPGAPGIAPFTILRPNGSLQVAFRPGEYRMVARPTGAP
                     PGTLPAVTWSRQMEIDPRVRGFKLQFNEADFK"
BASE COUNT          374 a          731 c          611 g          371 t
ORIGIN      
        1 cacgccatcg ttccgatagg gcgtctcgtg gtaggcccgc tgcgcgtcca cgtaggcgct
       61 cacgagattg accgcggtca gctcggtctt gctgatccga cgactcacga gatcctcgtg
      121 cccttcactg gagtcgaagc gccactgcgc tcccttcttg atgagcggaa tcggatatgg
      181 ctgctcgttc ttgccgagct gcagcaccac gcggctctcg tcctcggcga cgacctcgtg
      241 gccctcctgg tagagtgaaa ggaaccattc acgctcgtgc cgatcgatga ccggatcgcc
      301 cgagagcacc agccgcccgg ccttgtcgcc caggatctcg gccactgtct tgttatcccc
      361 cttcttgtaa gccttggcca gggccttcgt ggccgcctcc ggtgtggcaa agctcttctg
      421 cgggttaccc ttgccctgcc cgaaggctgc ggggaggaag agaaatccgg ccaccagcca
      481 taggacacat gcccgcagag tgcggcttat ggcgcaccga cccgtgcagg aacttcccat
      541 ccctctcatc aactcacctc ccctccatcc tggtcttgcc agtttcgcct ttccgattgt
      601 ctccctgatc tcgcggcttt cttctgtcac ttgccgggca agctgggagc catccccggc
      661 aacccctcgc ggatctgtat cgagaagtcg agcgccgagg caccccacgc gaccccgacc
      721 agcggggtgg gccagaccag cagtcgagcg agcaatgccc atcttcgact tcgccttgaa
      781 atcatcgcgc ctcattccca tcagggggca tggggcttgg ccttccacct gtccctcggg
      841 acccacacct cgggcaacac attcttttca atttcgaatt ccgatattcg agatcagggt
      901 ggttcttgtc agctgaaggc ggagtggcgg ctcaagaact tgggagagtc aggaacttct
      961 aggagcgggt gcgccgcccc tgcgcttcgt gacgaactgc cccaccgctc cgccgatggc
     1021 gcccggaacc gccccgaccg ccagtacctc ccacagggag ggaagcgtcg cagccccgag
     1081 aaaagctaac aagccgacga ggggcacgct gcccaggtaa aacaaaatgg ataatcccgc
     1141 tccgaccacg gcgccgagca agattctccc tgtcccccct gatattccac caatgacgat
     1201 ccccaaaacg gcggggagga ggataaccgc atattgctcc cccgtaatgg aaggaatatg
     1261 cagcaccgca acggccacgc gaatgacccc tccaactaat gcgcccacgc cgaacccgaa
     1321 ccacgagatt ctcgaggcca tcggaatctc ctttgccgcc gagctactcg acaatccgac
     1381 tcctggcggc gcccggccca tcggcggaac tcggtcgcaa ccggcgtgtc gtccgtcaga
     1441 ccgaggtgcg tcgtgcttac gacatccccc tcccctccgc cacgaacctg gatcgttcca
     1501 cagtgtggtt acttgaaatc cgcctcgttg aattgcagct tgaagccccg cacccgtggg
     1561 tcgatctcca tctgtcggct ccaggtcacc gccggcagcg tcccgggggg cgcccctgtt
     1621 gggcgcgcca ccatgcggta ctcaccgggc cgaaaggcga cctgaaggct gccgtttggc
     1681 ctcaggatgg tgaacggcgc gatgccgggg gctccggggt cctggttcag atacacgccg
     1741 atggtccagc gcgtcccgtt gacgagggta cccagggctg cggactggcc gccggccgag
     1801 cttggcttct gcgccgacac gacccgcacc cccgacgggg gcggacagcc cggagagcct
     1861 tcgtaggcct ggcgcacctc ctcctgttgg atggtcacct cggagaggcc gagggtggcg
     1921 acccccagga agaagcggcc gaagtatttc ccgtcgcggt ccgggacgga atcgcatcgc
     1981 gtgaccgcca ggctggcgca gcccgccagc gtcacgagta cggcaagcca gcaggcgagc
     2041 agacgcgcag tcggccacaa ccggcgcgtg ttcgatctcc cttcgag
//
LOCUS       DBNGZC010000683         3080 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1130767, whole genome shotgun sequence.
ACCESSION   DBNGZC010000683 DBNGZC010000000
VERSION     DBNGZC010000683.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1130767"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2767
                     /locus_tag="AC16G0_11805"
     CDS             <1..2767
                     /locus_tag="AC16G0_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5100332.1"
                     /translation="ANMPSPPTMRKVNPADWPIFFLSLSSPTLPLSVVDEYAETLLAQ
                     RISMVSGVSQVQVHGSQKYAVRVQVDPSAMAARGIGIDEVENAIAEGNVNLPTGNLDG
                     RHQTFAVEANGQLFDAAAYRPLIVTYRNGSPVRLGELGRVIDSVENDKQAGWRDTTRA
                     IVLAIQRQPGTNTVEVVDAIRALLPTFRAQLPASIALDTLYDRSQTIRASVADVKFTL
                     LLTICLVVMVIFLFLRNLSATIIPSLALPMSVVGTFAVMYLMGYSLDNLSLRALTLSV
                     GFVVDDAIVMLENIVRHMEAGEEAHEAALIGSREIGFTILSMTLSLAAVFIPVMFMGG
                     IVGRLFHEFAVTVTSAILISGFVSLTLTPMLCSRFLHPPREAHHGRLYAVSERFFQGM
                     LSTYRWSLERCLEHRPAVMGLSLLLLVATAYLFFVVPKGFIPTEDTGQIIGITEAAQD
                     TSFDSMVEHQQQVAAIVRKHPEIETFMSSLTSGGIGDTQNMGRLGIMLKPRAERKHSA
                     AEIIQELRHDLAAVPGMRVFLQVPPSIRIGGMMTKSLYQYTLQGADIEELYRVSRDMQ
                     ARITALPMLQDVTSDLQITSPKVRVEIDRDKASALGITANQVENALYDAYGSRQVSTI
                     YTPTNQYWVILELDPQYQQNPDALSLLYVRSSKGQLVPLNAVAKLTPGTGPLTVTHLG
                     QLPSVTLSFDLRPGISLGDAVAQINRVGRATLPPTISGTFQGSAQAFEASMQGMGLLV
                     VLAVLVIYIVLGILYESFVHPITILSGLPSAGVGALATLILFRHDLNIYGFVGIIMLV
                     GIVKKNAIMMIDFALYAQRQGKTAAEAIFQGSLLRFRPIMMTTMAALMATLPIAMGAG
                     QGAESRRPLGLAVVGGLVVSQLLTLYITPVTYIYLDSFGHWLRTRGWLRSTMSKGHES
                     VLDAPAP"
BASE COUNT          529 a         1086 c          893 g          572 t
ORIGIN      
        1 ggcgaacatg cccagcccgc ccaccatgcg gaaggtcaac cccgcggatt ggcccatctt
       61 cttcctgtct ctcagctcgc ccaccctgcc tctctccgtc gtggacgaat atgcggagac
      121 cctcctggcc cagcgtatct ccatggtaag cggcgtctcg caggtgcaag tccacggcag
      181 ccagaagtac gccgtgcggg tccaggtcga tccatcggcc atggcggcgc gggggatcgg
      241 gatcgacgag gtcgaaaacg ccattgcgga gggcaacgtc aatctgccga caggaaacct
      301 ggacgggcgt caccagacct ttgcggtcga ggccaatggg cagctcttcg acgcggcggc
      361 ctaccgcccg ctgatcgtca cctaccgcaa cggctccccg gtgcgcctgg gggagttggg
      421 ccgggtcatc gacagcgtgg agaacgacaa gcaagcaggc tggcgcgaca cgacccgcgc
      481 catcgtcctg gccatccaac ggcagccggg caccaacacc gtggaggtgg tggacgccat
      541 ccgggccctc ctgcccactt tccgggcaca actcccggcc tcgattgctt tggacacgct
      601 gtacgatcgg tcccagacca tccgcgcctc ggtggccgac gtgaagttca ccctgctcct
      661 caccatctgc ctcgtcgtga tggtgatctt cctgttcctc cggaacctct ccgcaaccat
      721 tatccccagc ctggccctgc ccatgtccgt cgtagggacg ttcgcggtca tgtacctcat
      781 gggttacagc ttggacaatc tctccctcag ggccctcacc ctctcggtgg gcttcgtggt
      841 ggacgacgcc atagtcatgc tggagaacat cgtacgccac atggaggcgg gagaagaggc
      901 ccacgaggcc gccctcatcg gctcgcggga gatcggcttc accatcctat ccatgacatt
      961 gtctttggct gccgtgttca tcccagtcat gttcatgggc ggcatcgtcg gccggctgtt
     1021 ccacgagttc gccgtgaccg tcacctcggc catcctgatc tccggcttcg tctcgctgac
     1081 cctcaccccc atgctctgca gccggttcct tcacccgccc cgagaggccc atcacggccg
     1141 cctctatgcc gtgagcgagc gcttcttcca gggcatgctc tccacctatc gctggtcgct
     1201 ggagcggtgc ctggagcacc gccccgccgt catggggctc tccctccttc tccttgtcgc
     1261 cacggcctat ctcttcttcg tggtccccaa agggttcatc cccaccgagg acaccgggca
     1321 gatcatcggc atcaccgagg ccgcccagga cacctccttc gacagcatgg tggagcacca
     1381 gcagcaggtg gcggccattg tccggaagca cccggagatc gagacgttca tgtccagcct
     1441 cacttccggc ggcatcggcg acacgcagaa catggggcgg ctgggcatca tgctgaagcc
     1501 acgggcggag cggaagcatt ccgcggccga gattatccag gaactgcggc acgacctcgc
     1561 cgccgtcccc ggcatgcgcg tgttcctgca ggttcccccg tccatccgga tcgggggaat
     1621 gatgaccaag agcctctatc agtacaccct gcagggagcg gacatcgaag agctgtaccg
     1681 ggtgtcacgg gatatgcagg cgcggatcac cgccctcccc atgttgcagg acgtcaccag
     1741 tgacctccag atcacgagcc ccaaggtccg ggtggagatc gaccgggaca aggcctccgc
     1801 cctcgggatc accgccaacc aggtggaaaa cgccctctac gacgcctacg gcagccggca
     1861 ggtctcgacg atctacacgc cgacgaacca gtactgggtc atcttggagc tggatccaca
     1921 ataccagcag aatccggacg cactttcgct gctctacgtc cggtcgtcca agggccagct
     1981 cgttcccttg aacgccgtgg cgaagctgac gccgggaacc gggcctttga cggtgaccca
     2041 cctcggtcag ctcccctcgg tcaccctctc cttcgacctc aggcccggga tctcgctggg
     2101 cgatgccgtg gctcagatca accgggtcgg gcgggccaca cttccaccca ccatcagcgg
     2161 caccttccag ggcagcgccc aggcattcga ggcgtcgatg cagggcatgg gcctcctggt
     2221 cgtcttggcg gtcctggtca tctacattgt cctggggata ctctacgaaa gctttgtcca
     2281 cccgataacc atcctgtccg ggctcccctc agcgggggtc ggcgccctgg ccacacttat
     2341 cctgttccgc cacgacctga acatctacgg ttttgtcggc atcatcatgc tggtgggcat
     2401 cgtgaagaag aacgccatca tgatgattga cttcgccctg tacgcccagc ggcaaggaaa
     2461 gaccgccgcg gaggcgatct tccagggctc cctcctccgc ttccgcccca tcatgatgac
     2521 caccatggcg gccctgatgg cgacgctgcc catcgccatg ggggcgggac aaggagccga
     2581 atcgcggcgc cccctgggcc tcgccgtggt cggcggccta gtggtctccc agcttctcac
     2641 cctgtacatc acgccggtca catacatcta cctggattcc ttcggccact ggctgcgcac
     2701 gcgtggctgg ctgcgctcca cgatgtcgaa gggccatgag tcggtgctgg acgcaccagc
     2761 gccgtagtac ccccgccatt ctaagactcc agcccggtcg gaacagggtg tccggagtct
     2821 tcagaagccc ctgccaagcc gccatctatc gatggctcag tcccctccag gaatagatac
     2881 cgaccggtca gtccaagcgt ccggggatcg cctgcgtcag gtgaggccta tggcggacgg
     2941 accgatgcga gccagcccct ggaaccgatc ctgccttgct ggtgttctgt atggttaaag
     3001 ccgtcgagga ccatgtcctg gtgtctctgc accggtccat caccgtcctc tgcggatcgt
     3061 catgcccgcc tgaagcatcg
//
LOCUS       DBNGZC010000684         1334 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_60333, whole genome shotgun sequence.
ACCESSION   DBNGZC010000684 DBNGZC010000000
VERSION     DBNGZC010000684.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_60333"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..>1334
                     /gene="hypF"
                     /locus_tag="AC16G0_11810"
     CDS             44..>1334
                     /gene="hypF"
                     /locus_tag="AC16G0_11810"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013320778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HIR5100333.1"
                     /translation="MPSTDERLRLRLMLRGAVQGVGFRPFVYRLANELHLPGWVSNSA
                     QGVTIEVEGVKQSLDAFLLRIGQDRPPRASIQSLETSYLDPAGFTAFTIRESDGRGPA
                     TALVLPDIATCPDCLREVLDPTNRRYLYPFTNCTNCGPRFTIIQALPYDRPNTTMRGF
                     SMCADCRREYEDPTDRRFHAQPNACPACGPQVVLWNPSGSILAARHEAVLQAADAIRW
                     GKILALKGLGGFHLLVDARNQEAVLRLRKRKHREEKPLAVLVPSIESAKALCQGSELE
                     ERLLCAPESPIVLLARRPDPPLDAIASGVAPRNPYLGVMLPYTPLHHILLQELGFPVV
                     ATSGNLSDEPICTYERDALPRLGGIADAFLVHDRPILRHVDDSIVRILMGRELVLRRA
                     RGYAPLPVHISQPAPPILAVGAHLKNSIAASVGTEVFV"
BASE COUNT          217 a          477 c          393 g          247 t
ORIGIN      
        1 tttcccgcct acaattgcgg cagaggatta tgtgtgtgac gatatgccga gcaccgatga
       61 acgcttgcgt ctccgtctca tgctgcgcgg cgcggtccaa ggggtcggat tccggccctt
      121 cgtgtaccgg cttgcgaacg agctgcattt gcccggctgg gtgtcgaact cggcccaggg
      181 ggtgaccatc gaggtcgaag gggtcaagca gagcctcgac gccttcctct tgcggatcgg
      241 gcaggatcgg ccaccccgcg cctctatcca gagtcttgag acctcctacc tcgatcccgc
      301 aggcttcacc gccttcacga ttcgggagag cgacggccgc ggtccagcca ccgccctggt
      361 cctcccggac atcgccacct gtccagactg cctgcgcgag gtcctggatc cgaccaaccg
      421 gcgctacctg tacccgttca ccaactgcac caactgcggt ccccgcttca cgatcatcca
      481 ggcgcttccc tacgaccggc ccaacaccac gatgcgcggc ttctcgatgt gcgccgattg
      541 ccgccgagaa tacgaggatc cgaccgaccg ccgctttcac gcccagccga atgcctgccc
      601 agcatgcggg ccgcaggtgg tgttatggaa tccgtccggc agtatcctgg ccgcacgaca
      661 cgaggccgtc cttcaggccg cggacgccat tcgctgggga aagatcctgg ccctgaaggg
      721 cctgggggga tttcatcttc tcgtggatgc tcggaatcag gaagcggtcc ttcgcctccg
      781 gaagcgcaaa caccgggagg agaagccgct cgccgtgttg gttccatcta tcgagtcggc
      841 gaaggccctc tgccagggct cggaactcga ggagcgcttg ctctgcgcgc ccgaatctcc
      901 gatcgtcctt ctggcacgcc gccccgatcc ccccttggac gcgatcgcgt ccggggtggc
      961 cccccgcaac ccgtacctgg gggtgatgct gccgtatacc cctctccatc acatcctcct
     1021 tcaggagctg ggattccccg tggtggccac gagtggcaac ctgtccgacg agcccatctg
     1081 cacctacgag cgggatgcgc tcccacgact cggcggtata gccgatgcct tcctagtcca
     1141 cgatcggccg atcctccggc acgtggatga ttccatcgtc cgaatcctca tggggcgcga
     1201 gctggtcctc cgtcgggccc gagggtacgc cccactgccc gtccacatct cgcagccggc
     1261 gcctcccatc ctcgccgtgg gagcacatct gaagaacagc atcgcagcct ccgtggggac
     1321 agaggtcttc gtca
//
LOCUS       DBNGZC010000685         1480 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1721287, whole genome shotgun sequence.
ACCESSION   DBNGZC010000685 DBNGZC010000000
VERSION     DBNGZC010000685.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1480)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1721287"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="AC16G0_11815"
     CDS             <1..412
                     /locus_tag="AC16G0_11815"
                     /inference="COORDINATES: protein motif:HMM:NF014671.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HIR5100334.1"
                     /translation="PLIRELYTEARAYRESRGIHAAALAEGEKLLLVTEDVGRHNAID
                     KVCGEAMLRGIPTEGKILLATGRISSEMLRKGAHMGTPVVVSRTSATTMSIDLAKRLG
                     LTLIGYVRGDSFYIYSHPERLVLPKPGRVLAGQV"
     gene            571..1128
                     /locus_tag="AC16G0_11820"
     CDS             571..1128
                     /locus_tag="AC16G0_11820"
                     /inference="COORDINATES: protein motif:HMM:NF019542.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HIR5100335.1"
                     /translation="MPDYYEFEVSLRNITPRIWRRFLLRKTATFHDLHMAIQEACGWW
                     NSHLFAFRAWGRENEEIAGMPDEETDEPTPDAKKVKLAPFFDKSDADKCLYEYDFGDG
                     WEHEVRLRKRVTLAESFKRRLLDGRRAFPPEDCGGVWGYERCVKILSGAAKGADEDAE
                     ELKEWLCDWTPEAFDLQAVKRTFDG"
     gene            1217..>1480
                     /locus_tag="AC16G0_11825"
     CDS             1217..>1480
                     /locus_tag="AC16G0_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010249914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5100336.1"
                     /translation="MIELARALRVEVEEAAVRLRAFGETDVTRDRGPGKWTRKEILGH
                     LIDSAANNHQRFVRAQLVSHFVWPGYDQESWVLLHRYRARPWME"
BASE COUNT          284 a          445 c          494 g          257 t
ORIGIN      
        1 tccgctcatc cgcgagctgt ataccgaagc ccgcgcctac cgagaatcgc gggggatcca
       61 cgcggcggcg ctggcggaag gggagaagct cctgctcgtg accgaggacg tgggccgcca
      121 caacgccatc gacaaggtct gcggcgaggc aatgctccgg gggatcccga cggaggggaa
      181 aatcctgctt gccacgggcc ggatctcctc ggagatgctc cgcaaggggg cccacatggg
      241 cacgccggtc gtggtctcgc gcacgtcggc cacgaccatg tccatcgacc tcgccaagcg
      301 gctgggcctt accctcatcg gctacgtccg cggcgacagc ttctacatct atagccaccc
      361 ggaacgcctc gtcctcccca agcccggacg agtgctcgcc ggccaggtct aggccaagtg
      421 ttcggcacgt ccctcctatc ttcatttcca acctgcatca ttccgtgacg ggtctcacgc
      481 gcatgtttgg gcttgccatc aacggaggaa gaatgccatc atcatcgcgg atgcttccat
      541 tgtgggtgtc tccagccaga gggagaggcc atgcctgact actatgaatt cgaagtgtcg
      601 cttcggaata tcacaccgcg catctggcgg cgattcctcc tccggaagac ggccacgttc
      661 cacgatctgc acatggcgat ccaggaggcc tgcggctggt ggaattctca cctcttcgcg
      721 ttccgggcgt gggggcgaga aaatgaagag atcgccggca tgcccgatga ggagaccgat
      781 gagcctacac cggatgcgaa gaaggtcaaa ctggcgccct tctttgacaa atctgatgca
      841 gacaagtgtc tgtacgaata cgatttcggc gatggctggg agcacgaggt gaggctccgg
      901 aaacgagtca cattggcgga gtccttcaag cgccgcctat tggatgggcg ccgcgccttt
      961 cccccggagg actgcggcgg ggtgtgggga tacgagcggt gcgtgaagat cctcagcggc
     1021 gccgcgaagg gtgcggatga ggacgctgag gagctgaaag aatggctctg cgactggacg
     1081 cccgaagcct tcgatctcca agccgtgaag cgcaccttcg acggctgatc ccccggtcgg
     1141 aggcgctgta tgaggcaaac gcaggggtga ggcgggtcag cccggcgcac ggcacgcgtt
     1201 cgggaggaag agacgcatga tcgagctggc gcgggcactg cgagtcgaag tggaagaggc
     1261 ggcggtgcgg ctcagagcct tcggggagac cgatgtcacg cgtgaccggg gccctggcaa
     1321 gtggacgagg aaggagattc tgggccatct gatcgactcc gccgccaaca accaccagcg
     1381 tttcgtgcgt gcgcagctcg tgagtcactt cgtctggcca gggtacgacc aggagtcgtg
     1441 ggtattgtta caccggtatc gcgcccgccc ctggatggag
//
LOCUS       DBNGZC010000686         3298 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2604170, whole genome shotgun sequence.
ACCESSION   DBNGZC010000686 DBNGZC010000000
VERSION     DBNGZC010000686.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2604170"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..581)
                     /locus_tag="AC16G0_11830"
     CDS             complement(195..581)
                     /locus_tag="AC16G0_11830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5100337.1"
                     /translation="MPRVLIVDDEPALRESLRAGLELAGYEVHEASDGAEGLEQAVRH
                     LPDAIFLDIRMPGLSGYEVCTRLRANPATTRIPVVFLTAVDDEELSRQVRNAGGTACL
                     IKPFRLPEVTALAKWLIAHAPGEAGV"
     gene            complement(616..951)
                     /locus_tag="AC16G0_11835"
     CDS             complement(616..951)
                     /locus_tag="AC16G0_11835"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR5100338.1"
                     /translation="MRKRSQEVGHRHFLRLPVLVSVKGRAAQFGETELQGVVRNIGGG
                     GLMAEFSVQIVPGSVVDLTLQTRQGPVPVTGEVAWTGTPGPQVAHGIALRGPRGEEFA
                     RELFQHEQG"
     gene            complement(1328..1813)
                     /locus_tag="AC16G0_11840"
     CDS             complement(1328..1813)
                     /locus_tag="AC16G0_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015829728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HIR5100339.1"
                     /translation="MQRNPLSLMRVVVLALAAALATGPAFAEKPEWAGGGKGKGKAHG
                     KKGGSGEEGGPRIEGYFGDGHRTHVREYYGGEFQAGRCPPGLAKKHNGCMPPGQAKKW
                     QIGQPLPREVIFYDLPPTLAVQIGVPPAGYRFVRVASDILMIAIGTGLVVDAIKDLGR
                     M"
     gene            complement(2258..2557)
                     /locus_tag="AC16G0_11845"
     CDS             complement(2258..2557)
                     /locus_tag="AC16G0_11845"
                     /inference="COORDINATES: protein motif:HMM:NF026928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm27 family immunity protein"
                     /protein_id="HIR5100340.1"
                     /translation="MALYHDLQTDETTLIGRWIVLDLDVEDDDVSERIKWLVANRLQR
                     LTTSPSGWEILYRDPRDGRFWELSSPQGAPEGWGPPRLRFLSLAEATAKYRLGAV"
BASE COUNT          608 a         1120 c         1007 g          563 t
ORIGIN      
        1 agcccgtgac aacgacgagg gtcaggcaac ctgcgaacag gctgaggaat ccccatcgca
       61 tcgcattcgc ctccagcagg gacgcgcccg cccccactca cgctcctcgc agggtgccct
      121 gaaagacggt atctccctcg gcgagagcga acgcggtctg aggtgccgcc agcccccggg
      181 cactcggcct gcgtctacac tcccgcctct cccggggcgt gggcgatgag ccacttggcg
      241 agcgccgtga cctcgggcag gcggaacggc ttgatgaggc acgccgtgcc ccctgcgttc
      301 cgcacctggc gggacagctc ttcatcgtcc accgcggtga ggaagacgac cgggatccgc
      361 gtcgtggccg ggttcgccct gagccgcgta cagacctcgt acccgctgag ccccggcatc
      421 cgaatatcga gaaagatcgc gtccgggagg tggcgcaccg cttgctccag tccctcggcc
      481 ccgtccgacg cctcgtggac ctcgtagcct gcgagctcca ggccggcgcg cagcgactcg
      541 cgcagggctg gctcatcgtc tacgatcagc acgcggggca tggtcgcttg cctccgagtc
      601 atccctaccg ttcggtcacc cttgctcgtg ctggaacagt tccctggcga actcctcccc
      661 gcgaggtccg cggagggcga tgccgtgggc aacctgcggg cccggcgttc ccgtccaggc
      721 gacttcgccg gtgactggga ccgggccctg gcgggtctgg agggtgagat ccacgacgct
      781 ccctgggacg atctggacgg aaaactccgc catcaaaccg ccaccgccga tgttccggac
      841 caccccctga agttccgtct cgccgaactg cgccgcccgg cccttgaccg acaccaacac
      901 cggcagccgc aagaaatgac ggtgccccac ttcttggctg cgcttcctca tcgttccctc
      961 cacactctcg tccctccggc ctctcgtcgg gagaaccgcc tgcgtggtcc aaggagaaga
     1021 tcacggccgc cttcgaaggt gaagccgccg gcaagggtgg tgcgaggcag gacaccgtcg
     1081 ctggtgaggt gggtccggcg ggtgaggaag tcgaatgagg ccaacttcac cccccggtcg
     1141 aactcgaacc agccgaaagc cactcgcagc cgcctccaca cgcctgaaaa tcagtgcggt
     1201 acggaacagg tagtgccagt ggtacaaggg ccagtgcgtg gcgcagaaag cgcggcacga
     1261 gatcggctgg aacgagtcag cccgtatgtg ggaatggtgc ctctatctcg ggccgggcgc
     1321 tgtggaccta catccgaccc agatccttga ttgcgtcgac gaccaagccg gttccgatgg
     1381 cgatcatcag gatgtcggag gccacgcgca cgaagcggta tcccgccggc ggaacgccga
     1441 tctgcaccgc gagcgtgggg ggcaggtcgt agaagatgac ctcgcggggc agcggctggc
     1501 cgatctgcca cttcttcgcc tgccccggcg gcatacagcc attatgcttc ttggccagac
     1561 ccggagggca ccggccggcc tggaattcgc ccccgtagta ctcgcgaacg tgcgtccggt
     1621 ggccgtcgcc gaagtaccct tcgatgcgcg ggccgccttc ctctcccgac ccccccttct
     1681 tcccgtgcgc cttgcccttg cccttgccac cgccggccca ctccggcttt tccgcaaagg
     1741 ccggacccgt cgccagggcc gcggccaatg ccagaacaac cacccgcatc aggctcaacg
     1801 gattgcgttg cacggcgtac ctcctccaag atgagattct tccgccacac atattgccgt
     1861 gctgatcaag gtgaaacgcg ccgcgatcca ctcgcgactg cctccaccca cttcaccgcc
     1921 agtgcagtgg aggtgatgca agtcgtacaa aggccggtgg atggccccaa agtgcggcag
     1981 aaatggaggg cgagtcttcc ggcaatgtgg agcagaggtc gggaggctcg ggtgggagcg
     2041 taacgctcac ctcctgtcgt gtcaatccga ttcccattct tgcttgggtg atcggacttc
     2101 tgtcgtggcg atcctccggc gagcaccccc ctgaccagga cgagtcccct ggcccgaaga
     2161 ccagcgggtt tgaaggggcg cccatcgcag aacctctggg ggaggtgcgg gtcacttgtg
     2221 cccactctgc gccgaggttg tccgtcgcat tcgcgtgcta gacggccccc aaacggtact
     2281 tcgccgttgc ctcggccaat gacagaaagc gcaaccgagg cggtccccag ccctccggcg
     2341 cgccttgcgg ggaggacagt tcccagaagc ggccatctcg ggggtcccgg tacaagatct
     2401 cccatccgct gggcgaggtt gtgaggcgtt gcaagcgatt cgcaaccagc cacttgatcc
     2461 gctcgctgac atcgtcgtcc tcgacatcca agtcaagaac gatccagcga ccgatcagtg
     2521 tcgtctcgtc cgtttgaagg tcgtgataga gcgccatctt gtcgccaagg ccatcaaaag
     2581 acagcaggtc gcgtgcgcgt gcttccgccc ctcgtcacag cgactcccct tactagggga
     2641 tcggttcatc ttttccagag accttccatt cccgttcgca aactgctccc gctagaacgc
     2701 cgtccagcgc acccagccgc ccgcaacgct ccttcagtcc cacatgctcc cctccattcg
     2761 gagtggcccg ccacccaccg acagtgcaac tgccatgccg ccattggcag caagtagatc
     2821 ggggcatccg cgatgcggct atccccgcac aagtgcggag gcggaaggcg caaggcgaga
     2881 ttggggaggt gttgagttcg gattagatcc tcagtatctg aagagattcg gcaatcggaa
     2941 aggatagaca aaaagacgga agttgctctc ggcgtcgtgg ggtggctaac gaaaccgcta
     3001 ccggcctccg aatagggctt actgtacggt ctctaattaa ggcgacagtc cgcttaaggc
     3061 gacagtccgc ctcaagaccg cacagtccgg agcgcagttc gaggggagga gcagatggga
     3121 atcaatacaa ccggcgaaag tcaccgcgga cgatacggtc ttctgaccga tcatgccccg
     3181 gcaatcagcc gctgaagcaa ctcaccatat gcatccgtga acgcgtgggc ggccctggaa
     3241 aagtcctgag cgtccgcggg gtcgatggtc gcaaaatctt cacagatttg acgatcgc
//
LOCUS       DBNGZC010000687         1636 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1821547, whole genome shotgun sequence.
ACCESSION   DBNGZC010000687 DBNGZC010000000
VERSION     DBNGZC010000687.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1821547"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..849)
                     /locus_tag="AC16G0_11850"
     CDS             complement(82..849)
                     /locus_tag="AC16G0_11850"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009768807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5100341.1"
                     /translation="MGVRFDGTVAIVTGSSDGIGKAAALILARDGAQVVLNARGEERL
                     EAARTAVQAVGKPPLALAGDVSQAAVVERLVGETLQQFGRIDVLVNNAGGGTPLRSLD
                     EVTEEDWSRVVDSNLKSAFLCCKAVAPAMRRQRHGRIVNVSSVAGRNVSRLSGPQYSA
                     AKAGLLGLTRHLAQDLGPSGITVNAVAPGPTLVDRFVKKWAAQSEADRARILANIPLG
                     RLAQPEEVAAVIAFLASDDARYVTGVTIDVNGGSFMS"
     gene            complement(945..>1636)
                     /locus_tag="AC16G0_11855"
     CDS             complement(945..>1636)
                     /locus_tag="AC16G0_11855"
                     /inference="COORDINATES: protein motif:HMM:NF015948.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR5100342.1"
                     /translation="THDQGGATLALKNLKGLVTDVEKRRIHQEGMFEGAVDLVSHFQP
                     VFAVVDGLIGQEGMGPLMGLPVEMRLLLAGRDLVAVDATAGRVMGFEPEEVPITKAAA
                     ERGLGVLEEGQIEVVGEQIESVRRRFVRCEEDHRIDRAGITVVHSEGTCTGCRNGLLS
                     SLFDMRAEGTLELARGLTIIAGPTAIPEGVPPEKLVSIGTCSWPEARRLPRHVRGCPP
                     NNVDIIRALSQ"
BASE COUNT          276 a          609 c          472 g          279 t
ORIGIN      
        1 tagctagtgc agcctgggga aaggtggaat gcccggagcg tcggacagga tgggccgtga
       61 aaagttcgac cgttcctccc gctacgacat gaaactcccg ccgttcacgt caatggtcac
      121 accggtcaca taccgtgcgt cgtccgacgc gagaaaggcg atgaccgccg ccacctcctc
      181 gggctgcgcc agtctcccca gcgggatatt cgccaggatg cgtgctcgat cagcctcgga
      241 ctgggcggcc cacttcttga cgaatcgatc caccagcgtg ggccctggcg ccacggcatt
      301 taccgtgatg ccggaggggc ccaggtcctg ggccagatgc cgcgtgagcc cgagcaaccc
      361 tgccttggcc gccgagtact gcggtcccga cagccggctc acgttgcggc cggcaaccga
      421 agaaacattc acgatgcggc cgtgacgctg gcgccgcatc gcgggcgcaa cggctttgca
      481 gcacaggaag gcgctcttca ggttcgagtc cacgacccga ctccagtcct cctccgtcac
      541 ctcgtcgaga gaacgcagag gggtgccgcc cccggcattg ttgaccagga catcaatgcg
      601 tccgaactgt tgcagggtct cgcccaccag ccgctccacc accgccgcct gactcacatc
      661 tccggccagg gcgaggggcg gcttccccac cgcctgaacg gccgtccggg ccgcctcaag
      721 tctctcctca ccgcgcgcgt tcaggaccac ctgggcacca tctcgcgcca agatcagcgc
      781 cgccgccttc ccgataccgt ccgacgatcc cgtgacaatc gcaaccgtcc cgtcaaagcg
      841 cacgcccatc tcctctccgc attccgcgat ttccttctac gaagggttct tcttccatgc
      901 cagcaattcg caattcgaaa ttcgaaatct ccccgttttc ccttctactg actcagagcc
      961 cggatgatgt ccacgttgtt gggtgggcag ccgcgcacat gccgcgggag ccgccgggcc
     1021 tcgggccaac tgcacgtgcc gatactgacc agcttctccg gaggcactcc ctccgggatc
     1081 gccgtcggcc cggcgatgat ggtcaggccg cgagccagct ccagggttcc ctctgcgcgc
     1141 atgtcgaaga gggagctgag cagaccgttg cgacagccgg tgcaggtgcc ctcgctgtgg
     1201 acgacggtga tgcccgcccg atcgatgcgg tggtcctcct cgcagcggac gaagcgtcgc
     1261 cgcacggatt ctatctgctc ccccaccacc tcgatctgcc cctcctccag gacaccaagg
     1321 ccccgctcgg ccgccgcctt ggtgatgggc acctcctccg gctcgaagcc catgacgcgg
     1381 ccggccgtgg catccaccgc caccaggtcc cgcccggcca agaggagcct catctccaca
     1441 gggagaccca tgagcggccc catgccttcc tggccgatga ggccgtccac cacggcaaag
     1501 accggctgga agtggctgac cagatccacg gccccctcga acatcccctc ctggtggatg
     1561 cgccgcttct ccacatcggt caccaggcct ttcagattct ttagggccag cgtggctccc
     1621 ccctgatcgt gggtct
//
LOCUS       DBNGZC010000688         2701 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1623212, whole genome shotgun sequence.
ACCESSION   DBNGZC010000688 DBNGZC010000000
VERSION     DBNGZC010000688.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1623212"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..136)
                     /locus_tag="AC16G0_11860"
     CDS             complement(<1..136)
                     /locus_tag="AC16G0_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HIR5100343.1"
                     /translation="MLKLFRHKKAKGFTLIELLIVVAIIGILAAIAIPNLLSAQKKSK
                     Y"
     gene            complement(411..>2701)
                     /locus_tag="AC16G0_11865"
     CDS             complement(411..>2701)
                     /locus_tag="AC16G0_11865"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR5100344.1"
                     /translation="RSTAWTGSSTTGTMARSDGRFLPMATLLLGAVQIALPWTVGGRS
                     AVGQVSLVLLLGLAGMIGLVLEGSSPRSRSSPPLLLAGVLIAISALHTIYLDRTVQSL
                     LLLLSYLLAATLAAHAARNAQWAKRILLAGISISGVLVSLAGMFQLLQGSKGGLYARL
                     LTGPFGYPNAAGGFLLLTAGAALAFAREERSPATRTASIAAALVSILGLALTRSHGVM
                     FAATVGLVIWALTDRTAWWSQRRVWVGAGVVGLLLIVALAPKWLIGLPLRLWSLFDTG
                     AADPSFMWRLHILEWTWAMVRDHPWWGVGPGAFPVALTHYQRIPYVSGMNPHNLYLEL
                     AAEFGLPTAILAMLALGRLFFRMGTAIRGTPVIHPSRGHLVALLATLAAFAVHSAMDL
                     DWSFPAIAVTAAILLGMASAQLARGVGMAPWSPILWRWSLLSLLVVSAVLALSRFYSA
                     MLVEGAQLALTTGDAAAARADLSWALRLNPLSFPARQMMTRARMQSGDPQGALEVAEH
                     AIRVAPLDPNSYYLAGEIAAARERWGSAVDFFEKAAERAPSAQLRFHASLVETAARAG
                     RGAEARLWYEKAVDTFTPERVLEPEARCLVPGDRYVLARMSRIASRLYAEAGDRMREQ
                     VAADLAWLLAQPDLRGFCVTGGWPDQASPEAAMVNFWRALSEGGWLWAERYLSPELRT
                     LGRSIANGGRGGEGQPSRGRVAWIASLSGSESRANLRYEAEITKDPSQQYTRCAQTTL
                     RLIGESWFVERLPFLEPNPCKP"
BASE COUNT          532 a          836 c          891 g          442 t
ORIGIN      
        1 catacttcga cttcttctgg gcggacagca ggttcgggat ggcgatggcc gccagaatgc
       61 cgatgattgc cacgacgatc aacaactcga tcagggtaaa ccctttggcc ttcttgtgtc
      121 tgaacagttt cagcatcgaa gccctccttg taggaaatag ttgctctggt tcgagccgga
      181 tgacccgtcc tctccctgag tgggaggggg gtgacatact cgtcaggccc accttgtgca
      241 gcggctgtgc caaaggagaa ccgaggtcat gggaatgagc tattgttgag gatagattgt
      301 aaaattaatg agttatatga taagaaatag ccgagtggtg acgtcctgtt taatctgcac
      361 cggccatcct cctgaagtgt tttgtcggga gtggacatta tttgtcaatc tcatggtttg
      421 caggggttcg gctctagaaa cgggagccgc tcgacgaacc agctctcccc gatgagccgg
      481 agtgtagttt gggcacagcg ggtatactgc tgcgaaggat ccttcgttat ctccgcctca
      541 tagcgcagat ttgctcggct ttcacttccg ctcagcgagg cgatccacgc cacacgccct
      601 cggctgggct gtccctctcc ccctcttcct ccgttggcga tacttcttcc gagggtgcgc
      661 aactcggggg acaggtatcg ctcggcccag agccatccgc cctcggagag ggcacgccag
      721 aagttcacca tggctgcttc cggggatgcc tggtcgggcc accccccggt gacgcagaat
      781 ccccgaagat ccggttgggc aagaagccag gcgagatcgg cggcaacctg ttctcgcatg
      841 cggtcgccgg cctccgcata caggcgagag gcgattcggc tcatgcgtgc cagcacgtag
      901 cgatcacccg gcacgagaca gcgggcttcg ggctccaaga ctcgctcggg agtaaaggtg
      961 tcgacggctt tttcgtacca cagccgagcc tcagctcccc gaccggcgcg cgcggccgtc
     1021 tcgacaaggc tggcgtgaaa gcgaagctgg gcggagggtg cccgttcagc cgccttctcg
     1081 aaaaaatcca ccgccgaccc ccaccgctcc cgggcggcgg cgatctcgcc agccaggtag
     1141 tagctgttgg gatccagggg ggcaacgcgg atcgcgtgtt ccgcgacctc cagggctcct
     1201 tgaggatcgc cggattgcat ccgagccctg gtcatcatct gccgcgcggg aaaactcagg
     1261 ggattcagac gaagggccca cgagagatcc gcgcgtgccg ccgcggcgtc cccggtcgta
     1321 agagccagtt gggcaccctc cacaagcatg gcggagtaaa agcggctgag cgcgaggact
     1381 gcggagacaa ccagcagcga gagcagactc catcgccaca ggatcggaga ccagggcgcc
     1441 attcctaccc cccgtgcgag ttgcgccgag gccatcccga ggaggatggc ggcggtgacc
     1501 gcaatcgcgg gaaagctcca gtccaagtcc atggcgctgt ggactgcaaa ggccgccagc
     1561 gtcgccaaca gagccaccag gtgtccgcgg ctgggatgga tcaccggggt gccccggatc
     1621 gcggtcccca tccggaagaa aagacgaccg agagcgagca tcgcgaggat tgcagtcggg
     1681 agaccgaact ccgccgccag ctccaggtag aggttgtggg ggttcatccc gctgacgtac
     1741 gggatacgct gatagtgcgt cagcgccacg ggaaacgccc ccggccccac gccccaccat
     1801 ggatgatccc gcaccatggc ccacgtccat tccaggatgt ggagtcgcca catgaaggaa
     1861 gggtcggcag cacccgtgtc gaagaggctc cataagcgaa gggggaggcc gatgagccac
     1921 ttcggcgcaa gcgcaacgat taggaggaga ccgaccactc ccgcccccac ccatacacgc
     1981 cgctgtgacc accaggcggt gcggtccgtc aaggcccaga tcacgagccc gacggtcgcg
     2041 gcgaacatga cgccgtgcga tcgagtcagg gccagcccca ggatcgacac gagggcggca
     2101 gcgattgagg ctgtccgggt cgccgggctc cgctcctccc gggcaaacgc cagggcggct
     2161 ccggcggtca gaaggagaaa cccgccggcc gcgtttgggt atccgaaggg gccggtgagg
     2221 agccgggcgt acaatccccc cttacttccc tggagtaact ggaacatgcc ggcgaggctc
     2281 accaggaccc cggagatcga gatccctgcg aggaggatac gcttggccca ctgggcgttc
     2341 cgggcagcgt gcgcagccaa ggtggccgcc aggaggtagg agaggagaag gagcaggctc
     2401 tgcaccgtcc ggtcgagata gatcgtgtgg agcgccgata ttgcgatcag gacgccggcc
     2461 aggaggagcg ggggcgagga tcgtgaccgc ggactggatc cttcaaggac aaggccgatc
     2521 atccccgcca ggccgaggag caggacgaga ctcacttggc cgacggcgct cctcccgccg
     2581 acggtccatg ggagggcgat ctggacggcg cccaggagga gtgtggccat ggggaggaag
     2641 cggccgtcag acctcgccat ggttccggtc gtggatgaac cggtccaggc cgtacttctt
     2701 c
//
LOCUS       DBNGZC010000689         2335 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2502714, whole genome shotgun sequence.
ACCESSION   DBNGZC010000689 DBNGZC010000000
VERSION     DBNGZC010000689.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2502714"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="AC16G0_11870"
     CDS             complement(<1..271)
                     /locus_tag="AC16G0_11870"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5100345.1"
                     /translation="MRFQGKTALITGGGRGIGATTALMMLAEGGGVGVVDVNRDHLSR
                     VVETAQARGYKLKTFLADVSKMDQVRTAVDAFVKEFGRIDNMVNNA"
     gene            complement(331..1131)
                     /locus_tag="AC16G0_11875"
     CDS             complement(331..1131)
                     /locus_tag="AC16G0_11875"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR5100346.1"
                     /translation="MPPTSQYAAPAVEAALSILETLGTVHEMGVTELAKRLGLGKSSV
                     YRLLATLSRRGYVEKNPQSDRYLLTHRLFAVGSRATDRLGLQDIAHPIMQRLASETGE
                     AVNLGVLDGTRVVNLHRVESPHLLRVHLEVGGGVPAHATALGKVLLAFLGEAAAMRRL
                     QGHRLERVTPRTIGDRTALRTALRRIREQGYALDDEECSLGLRCVAAPIVDHRGSVAA
                     ALSVSGPSQRLSPSTLQALAGRVQAAARDISMRLGHPPVGSPDRGGER"
     gene            1405..>2335
                     /locus_tag="AC16G0_11880"
     CDS             1405..>2335
                     /locus_tag="AC16G0_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100347.1"
                     /translation="MTTNSRAVDLDVLRGRLTQGQRAILDAVWEHYRDQNRWIPRHVL
                     RERFGAPVLTAALNQLGGGVVAECQDDDEECYRLTFLGVLLVDQGQESEELLVRYLTF
                     VRDRFRSDPRVEWVGSQEVEAALGLTRERSRLLRQLIRLSHWWGGGSGFGGQEWSVGV
                     PIDVDDLPSEPDLRGYVRDHVLRHFAPELFLGETGGLSLRVQVGRDERPRDAFWFVRD
                     PALRRQLAMDWHEAQDVRQVRGWKSCVILCGGILEALLQDALTRAASPGTQDAPATDG
                     MNLADLVEAAITLGILGQGALPLGHALLGFRHLI"
BASE COUNT          375 a          815 c          748 g          397 t
ORIGIN      
        1 ccgcgttgtt caccatgtta tcgattcgcc cgaactcttt gacaaacgca tccactgcgg
       61 tccggacctg atccatcttg gacacatccg ccagaaacgt cttgagcttg tacccccggg
      121 cctgggccgt ctccaccacc ctgctcaggt gatctcggtt cacatccacg acccccactc
      181 ctccgccctc ggcaagcatc atgagggcag tggtggcgcc gatcccccgt ccccctccgg
      241 tgatcaaggc tgtcttcccc tgaaatctca tcccgtcacc tcctcttcag cgttggtcat
      301 tggccactgg attttcgtca ttcccccggg ttatctctca cctcccctgt caggcgatcc
      361 gacgggagga tgacccaggc gcatcgagat gtcccgcgcc gcggcctgga cccgccctgc
      421 cagcgcctgc aaggtcgatg gggacagacg ctggctgggt ccagagacgc tcaaggcggc
      481 cgccaccgat ccccggtgat ccacgatcgg cgcagccacg cagcggagac cgaggctgca
      541 ctcctcgtca tcgagggcgt agccctgctc tcggatgcgt cggagggcgg tccggagtgc
      601 ggtccgatcc ccgatggtcc gtggggtcac ccgttctagt ctgtggcctt gcagccgtcg
      661 catcgccgcc gcctctccca ggaacgccag caggaccttg cccagggctg tggcatgggc
      721 cggcacgcct ccccccacct ccaggtggac gcgcagcaga tgcggactct ccacccggtg
      781 caggttcacg acccgcgtcc catccaggac gccgaggttc accgcctcgc cggtctcgga
      841 agccaggcgc tgcatgatcg gatgggcgat atcctgcaaa ccgaggcgat cggtcgctcg
      901 gctgcctacg gcgaagagtc ggtgagtcaa caggtaccgg tcactctgcg gattcttctc
      961 gacgtacccg cgccggctca aggtagccaa cagccgatag acggaactct tccccaagcc
     1021 gagccgcttg gccagctcgg tcacccccat ctcatggacg gtccccaggg tctccaggat
     1081 ggacaaagcc gcctcaacgg cgggcgcggc gtattgagac gtgggaggca tgggactccc
     1141 ggctgggagg ctcgacgtcc aactggtgga acacagttca atatatgcaa caccacctct
     1201 cccgtcaagc gcccccagcc ccatggtcga ctttctgcag tcgaggacac aaagacgtgt
     1261 gggcccctgc cggacgttga cacccacctc gcccggagag atactcgctc caccttcagg
     1321 acgtggagaa ggggcgcggg gggtgcgcgc gccctgcacc aggctggggc tcgcccgatc
     1381 tcgggcggcc ggcgggggag ggagatgacg acaaacagcc gggcggtcga cctggatgtc
     1441 ctccgaggta gactgacgca aggccagcgg gcgatcctcg acgcggtctg ggagcactac
     1501 cgcgaccaga atcgctggat cccacgccac gtcctccggg agagattcgg cgcgccggtg
     1561 ctcaccgcgg cactcaacca gctcggaggg ggcgtggttg ccgaatgcca ggacgacgac
     1621 gaggagtgct accggctgac cttcctcggg gtgctcctgg tcgaccaggg acaggagtcg
     1681 gaagaactcc tggtccgcta cctcacgttt gtgcgcgatc ggttccggtc ggaccctcgc
     1741 gtcgagtggg tggggagcca ggaggtcgag gccgccctgg gtctcacccg cgagcggtcg
     1801 cgcctcctgc ggcagttgat tcggctgagc cactggtggg gcggcgggag cgggttcggc
     1861 ggccaagagt ggtccgtcgg cgtgcccatc gacgttgacg atctcccgtc cgagcccgat
     1921 ctacggggct acgtccggga ccatgtcctc cgacacttcg cgcccgagct gttcctcggc
     1981 gagaccgggg ggctcagcct gcgcgtccag gtcggtcgcg acgagcgccc ccgtgacgct
     2041 ttctggttcg tcagggatcc cgccctgcga cggcaattgg ccatggactg gcacgaggcc
     2101 caggatgtcc gccaggtgcg cggctggaag agctgtgtca tcctgtgcgg cggcatcttg
     2161 gaggccttac tccaggacgc cctgacccgc gccgcatcgc ctggaaccca ggatgccccg
     2221 gcaacggacg gaatgaacct ggcggatctg gtggaggcgg ccatcacgct gggcatcctc
     2281 ggccagggcg cacttcccct cggccacgcc ctgctcgggt ttcgacatct catcc
//
LOCUS       DBNGZC010000690         3374 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2604494, whole genome shotgun sequence.
ACCESSION   DBNGZC010000690 DBNGZC010000000
VERSION     DBNGZC010000690.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2604494"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(769..1674)
                     /locus_tag="AC16G0_11885"
     CDS             complement(769..1674)
                     /locus_tag="AC16G0_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012765412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5100348.1"
                     /translation="MDVTQGQMPARRRSGKTWVRLHSIPILIVVPALVIAVFLLTALL
                     AEHLVPADPLQQHLDYRLSPPYWLPDETNADRPFYILGSDQLGRDVLSRVIQGSRVSL
                     VVGFAAVFIGGTFGSSLGMLAGYMGKSIDDVIMMVADIQLAFPFILLAIAVIAVLGPS
                     FTNLVVLIGISGWVTYARIARAQVLSIKEKEYVEAIRCLGGNNVRIILRHLLPNALSP
                     LIVVATLDLARTIILESTLSFLGLGIQPPTPSWGGMLGEGRQYLGNAWWISIFPGLFL
                     MAVALSVSRIGDWLRDVLDPTLRSG"
     gene            complement(1687..2589)
                     /locus_tag="AC16G0_11890"
     CDS             complement(1687..2589)
                     /locus_tag="AC16G0_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5100349.1"
                     /translation="MRRLIRAVIVLWGISTIVFVVMHLSGDPVTLLLPQDATRADIDR
                     VTHQLGLDKPLWTQYVIFMANLARLDFGTSIHLRQPAFALALERLPATFELAAAAFCL
                     AVAVAVPIGVLAAMRPYSPFDSLAMLFAAIGQSAPTFFLGIMLILVFSLRIGYFPTSG
                     RGGLDHLVLPAITLGAYAMASIARLTRAAMLEVIRKDYIRTARAKGIAESVIVWRHAF
                     KNTAIPVVTIMGIQLGALLGGAVVTETVFSWPGIGRLAIQSIYNRDYAVVQATVFLTA
                     FWFVLINFAVDVIYGWLDPRIRYS"
     gene            complement(2671..>3374)
                     /locus_tag="AC16G0_11895"
     CDS             complement(2671..>3374)
                     /locus_tag="AC16G0_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010586097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5100350.1"
                     /translation="QPGFLQWLRALTSELGLVLIFDEVITGFRVAPGGAQQYFRVTPD
                     LAVLAKILAGGLPGGAVVGRADILEYLTFKTGDTRWNRYHRIAHPGTFNGNPVSASAG
                     VAALKIVATGKPQAEAAELGRVLRGGMNQVLDRRGIPGCVYGDFSMFHIYLGECPQGA
                     DCDRSLCTNDPTALMLARKGPRAEGLRRHMLAHGADIQGAGGMLSSAHTLADVERATD
                     IFDAALGDLADEGLL"
BASE COUNT          666 a         1005 c         1133 g          570 t
ORIGIN      
        1 ggtgggatct gcgtgctgaa ttgaaggtta catactacgc ccctcctgac acaggcaagc
       61 aaaaaatctc cgacacgttc ctggctgttc tcccgcaaat cttgtagtct tattgagcgg
      121 aaactcatgg aagccgagac ccggatacca tggggcggat ggagcctatg accgggtcat
      181 tctcctcgtg ggacggcttc caggagcagg cgaagcttcg aagatccgca aggtgtctgg
      241 agtgcgtctg agaatatgtc gaggatagag gaagtcgcgc tcgccgtact cgtcgtcaac
      301 ctcccattcg gcttctggag ggcgggcgtg cggaagctca ccctgccttg gattctggcc
      361 gttcatctgc cggttccact cgtcgtcggg ttgcggatcc tgtcagggct gggatggcaa
      421 ctctccacgg tcccagtcct gggtagcgcg ttttttgctg ggcagttttt cggaggaagg
      481 atccgccact ggtgggatag aaagtgcgaa gcccgatccg atgccggcgg gtagggccct
      541 ggggacatga ctgtcacccc aagtctctcc tcccttccac ctcctccacc tgccgagggt
      601 gctactgagc cctcgtggtg agcatctggg cgaccgatgc tggttcgggc ggtatcctcc
      661 ccttcactcg agacgagagt ggcacggttg tttgcaagag atgaggcgga ctgcagccca
      721 ggatgatgcc tcggccgttg ggagccaagt gggggcgctg tcgctttgtc acccggatct
      781 cagcgtcgga tccagcacgt cccggagcca gtcgccgatg cggctgacgc tgagggcaac
      841 cgccatcaag aacaggcccg gaaagatgga gatccaccac gcattcccca gatactgccg
      901 gccttcgccc agcatgccgc cccaactcgg cgtcggaggc tggatgccca gcccgaggaa
      961 gctgagggtg gattccagga tgatggtgcg ggccaggtcc agggtggcca caacgatgag
     1021 aggggaaagg gcattgggga gaaggtgacg gaggatgatg cgaacattgt tgccgccgag
     1081 acagcggatc gcctcgacgt attccttttc tttgatggag agcacctgcg cccgggcgat
     1141 gcgggcatac gtgacccagc cgctgatgcc gatcagcacc accaggttgg taaagctggg
     1201 gccgaggacg gcgatgacgg cgatggccag caggatgaag ggaaaggcga gttggatgtc
     1261 cgccaccatc atgatcacat cgtcgatgct cttccccatg tagcccgcca gcatgcccag
     1321 ggaagaaccg aaggtgccgc cgatgaaaac ggcggcgaag ccgacgacca gggaaacgcg
     1381 gctcccctgg atgacccggc tgagcacgtc cctgccaagt tgatccgagc cgaggatgta
     1441 gaatggccgg tcggcatttg tctcatctgg cagccaatag ggcggggaga gacgatagtc
     1501 gagatgctgc tggagcgggt cggcgggaac gaggtgttcg gcgaggagcg ccgtgagcag
     1561 aaagaccgcg atcaccaggg ctgggacgac gatgaggatt gggatcgagt gaaggcggac
     1621 ccaggtcttc cccgaccggc gcctggctgg catttgccct tgggtgacgt ccatgcttcc
     1681 ccgctcctag gagtaacgga tcctgggatc cagccatccg tagatgacat ccacggcgaa
     1741 gttgatcagc acgaaccaga aggccgtcag gaacaccgtg gcctgcacca cggcgtaatc
     1801 gcggttgtag atcgactgga ttgccaggcg gccgatgccg ggccaggaga acacggtctc
     1861 ggtgaccacg gcgccgccca ggagggcgcc caattggatg cccatgatgg tcaccacggg
     1921 gatggcggtg ttcttgaagg cgtggcgcca cacgatgacg ctctcggcaa tccccttggc
     1981 gcgggcggtc cggatgtagt ccttgcggat cacctccagc atggcagctc gggtgagtcg
     2041 tgcgatgctg gccatggcgt aggcacccag ggtgatggca ggtagcacca ggtggtcgag
     2101 gccgccccgg ccactcgtcg ggaagtaacc tatgcgcagg gaaaagacca ggatcagcat
     2161 gatccccagg aagaatgtag gggccgactg gccgatcgcg gcgaacagca tcgccaggct
     2221 gtcaaaggga gaatagggcc tcatggcggc caggacgccg atcggtacgg cgaccgccac
     2281 ggccagacag aaagccgcag cggccagctc aaaggttgcc gggagccgct ccaaggccaa
     2341 ggcgaaggcc ggctgacgca ggtggatgga ggtgccaaaa tcgaggcgag ccaggttggc
     2401 catgaagatc acgtactgag tccagagcgg cttgtccaga cccagttggt gggtgactcg
     2461 atcgatgtcg gcccgggtgg cgtcttgcgg gagcagcaat gtcacggggt cgcccgagag
     2521 gtgcatcacg acaaagacga tggtggagat gccccagagg acgatgacgg cccgtatcag
     2581 ccgacgcaac aggaaccggc gcaatggttg tacctcgcgg ggacagctcg cgaacggggc
     2641 agaggcaaga gcccgcgcca gggcggcggg ctacaagagc ccctcgtctg ccaagtctcc
     2701 cagcgccgca tcgaagatgt cggtcgcgcg ctccacatcc gccagagtgt gggccgagga
     2761 gagcatgcct cccgctccct ggatgtcggc gccgtgggcc agcatgtgac gtcgcaggcc
     2821 ctccgccctg ggccccttgc gtgccagcat gagggccgtg gggtcgttgg tgcaaaggct
     2881 gcgatcgcag tccgcccctt gcgggcattc ccccaggtag atgtgaaaca tggagaaatc
     2941 gccgtagacg cagccgggga tacctcggcg gtcgagcacc tggttcattc ctcctcgcag
     3001 gacccgaccc agctccgccg cttcagcttg aggcttcccc gtggccacga tcttgagcgc
     3061 cgcgacaccg gcagaggctg acaccgggtt gccattgaag gtccctgggt gggcgatcct
     3121 gtggtagcga ttccacctgg tatcccccgt cttgaacgtc agatactcca ggatgtctgc
     3181 ccggcccacg acggctcctc ccggcaatcc tccagccagg atcttggcca gcaccgccag
     3241 gtccggtgtc accctgaaat actgctgcgc accgcccggg gcaacgcgaa agcccgtgat
     3301 gacctcgtca aaaatcagca cgaggcccag ctctgacgtg agggctcgaa gccactggag
     3361 aaagcccggc tgca
//
LOCUS       DBNGZC010000691         3073 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1821828, whole genome shotgun sequence.
ACCESSION   DBNGZC010000691 DBNGZC010000000
VERSION     DBNGZC010000691.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1821828"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="AC16G0_11900"
     CDS             complement(<1..305)
                     /locus_tag="AC16G0_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HIR5100351.1"
                     /translation="MGAMSSIYGLMAEFDTPEDVLAAAKRAQAQGYRRMDAYTPFPVE
                     GLSEAIGFHHTWLPLLVLIAGIIGAIVGFASQYYATVIDYPLNVGGRPLNSWPSYIP"
     gene            complement(309..1730)
                     /gene="nrfD"
                     /locus_tag="AC16G0_11905"
     CDS             complement(309..1730)
                     /gene="nrfD"
                     /locus_tag="AC16G0_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR5100352.1"
                     /translation="MAAEGHEQAAGVQAQPSIIGPGYTYGSIGDKIAGAVFTTYTPWS
                     WVVGFAVGFFLLQVLMIAVAYLIYRGVGIWGINIPVGWGFAIVNFVWWIGIGHAGTLI
                     SAILLLLHQEWRTSINRFAEAMTLFAVSCAGIFPLIHTGRPWFAYWLLPIPNTLPLWP
                     QPRSPLVWDVFAVSTYATVSLLFWYVGLIPDLATFRDRAKHRLLQIVYGMLALGWRGS
                     AMHWHRYETAYLLLAGLATPLVVSVHSVVSFDFAVGIVPGWHTTIFPPYFVAGAIYSG
                     FAMVLTLTIPIRKIYGLEDFITMRHLRNMANVMLATGLIVAYGYLMETFMGWFSGNPY
                     EGYAIWNRMTGPYAPLYGLLILCNILVPQALWSDRVKRSVPAVFLISLVINVGMWLER
                     FVIVVVSLHRDFMPSAWGMYYPTIWDWATYLGTIGLFLAMIFLFIRVLPMISIFEMRT
                     LVAKGTRGQVPPGRPAPTPAAGD"
     gene            complement(1735..>3073)
                     /locus_tag="AC16G0_11910"
     CDS             complement(1735..>3073)
                     /locus_tag="AC16G0_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5100353.1"
                     /translation="PRPVSLKAGGGVSGPGAGAATAPAQSADGLEIVFRPDPAIWDGR
                     FANNGWLQELPKPLSKLTWDNTALLSPATAERLSLATGDVVELDLGGRTVRAPVWIMP
                     GHAVDSVTVHFGYGRTRAGRVGTGTGFNAYAIRTAAAPWSAPGLQVRKTGEQYPLATT
                     QSHYRMEGRNLIRMGTLAEYQKNPEFAREMAEEPPPELTLYPGFKYEGYAWGMAIDLN
                     ACTGCSACVVACQAENNSPIVGKDQVTRGREMHWLRIDRYYGGSLDNPEIHHEPVMCQ
                     HCENAPCEAVCPVGATNHSAEGLNDMVYNRCVGTRYCSNNCPYKVRRFNFLAFQDYNT
                     SSLKLMRNPNVTVRSRGVMEKCSYCVQRINAAKIEAEKADRRVRDGEILTACQQACPT
                     RAIIFGNINDPGSQVAKLKAEPRSYGILTELNTRPRTSYLAKVRNPNPEIEKG"
BASE COUNT          591 a          958 c          988 g          536 t
ORIGIN      
        1 atcggaatat acgacggcca gctattcaac ggacggccac caacgttgag gggataatcg
       61 atgaccgtcg catagtactg ggatgcgaat ccgacgatgg cgccgatgat gccggcgatc
      121 agcaccagca ggggaagcca ggtgtgatgg aagccgatgg cctcggacag tccctccacc
      181 gggaaggggg tgtaggcgtc catgcgacgg tatccctggg cttgggcgcg cttcgccgcg
      241 gccagcacgt cctcgggtgt gtcgaactcg gccatcaggc cgtagatcga agacatcgca
      301 cccatgcctc agtctccggc tgccggtgtg ggcgccggcc tgccgggagg gacctgtcct
      361 ctcgtgccct tcgccaccag cgttcgcatc tcaaagatag agatcatggg gagaactcgg
      421 atgaagagga agatcatcgc caggaacagg ccgatcgtcc ccaggtatgt cgcccagtcc
      481 cagatcgtcg ggtagtacat gccccaggcc gagggcataa agtcccggtg caggctgaca
      541 accacgatga cgaatcgctc cagccacatc ccaacattga tgacaagcga gatcaagaac
      601 accgccggga cgcttcgctt gactcgatct gaccagagcg cttgcggtac gaggatattg
      661 cagaggatga gtaacccgta gagcggagcg taggggccgg tcattcgatt ccagatcgca
      721 tacccttcgt acggattccc gctgaaccac cccatgaagg tttccatcag gtacccgtac
      781 gccacgatca accccgtcgc cagcatgacg ttcgccatgt tgcgcaggtg gcgcatggtg
      841 atgaagtctt cgaggccata gatcttgcgg atgggaatgg tgagggtgag caccatggca
      901 aatccggagt agatggcccc tgccacgaaa tacggcggga agatcgtcgt gtgccagccc
      961 gggacgatac cgacggcgaa gtcgaagctg acgacgctgt gcaccgacac caccaagggc
     1021 gtcgcgaggc cggccagcag cagataggcc gtctcgtacc ggtgccagtg catggccgat
     1081 ccccgccagc cgagggccag catcccgtag acgatctgga gcaggcggtg ctttgcacga
     1141 tcgcggaagg tcgccaaatc agggatcagc cccacgtacc agaacagcaa cgacacagtg
     1201 gcgtaggtcg agacggcgaa cacgtcccag accagcgggc ttctgggctg cggccagagc
     1261 ggcaaggtgt tggggatggg cagtagccaa taggcgaacc acgggcgtcc cgtgtggatc
     1321 aagggaaaga tacccgcgca agagaccgcg aacagagtca tggcctcggc gaaccgattg
     1381 atcgaggtgc gccactcctg gtggagcagg agcaggatcg ccgagatcaa cgtgccagcg
     1441 tggccaatcc cgatccacca gacaaagttg acgatggcga atccccagcc caccgggatg
     1501 ttgatacccc agatcccgac gccgcggtaa atcaagtacg cgacagcgat catgaggacc
     1561 tgaagcagga agaagccgac ggcaaacccg acaacccagc tccagggggt gtacgtggtg
     1621 aagacggcgc ccgcgatctt gtcgccgatc gaaccgtagg tatatcccgg cccgatgatt
     1681 gaaggctggg cctggacgcc tgcagcttgc tcgtggcctt cggccgccat gaagctatcc
     1741 cttctcgatc tctggattgg ggttgcgcac cttcgccaga tagctcgtgc gagggcgggt
     1801 gttcagttcc gtcaggatgc catagctccg gggctcggct ttgagcttgg ccacctggct
     1861 ccccggatcg ttgatgtttc cgaagatgat cgcccgggtg gggcaggcct gctggcaggc
     1921 cgtgaggatt tccccgtccc ggacccggcg atccgccttt tcggcctcga tcttggccgc
     1981 attgatccgc tggacgcagt acgagcactt ctccatgacg ccgcggcttc gcaccgtgac
     2041 gttcgggttt cgcatcagct tcaagctgga cgtgttgtag tcctggaacg ccaggaaatt
     2101 gaagcgccgg accttgtagg ggcaattgtt cgagcagtag cgggtgccga cgcaccgatt
     2161 gtacaccatg tcgttcaacc cctcggcgct gtgattggtg gctccaacgg ggcagaccgc
     2221 ctcgcagggc gcgttctcgc aatgctggca catgacgggc tcgtggtgga tctccggatt
     2281 atcgaggctg cccccgtagt agcgatcgat gcggagccag tgcatctcgc ggccgcgggt
     2341 cacctgatct ttccccacga tgggactgtt gttctcggcc tggcaggcga ccacgcaggc
     2401 actgcacccc gtgcaggcgt tcaaatcgat cgccatcccc caggcgtacc cttcatactt
     2461 gaatccgggg tagagggtga gctccggcgg cggctcttca gccatctcgc gggcgaactc
     2521 ggggttcttc tggtactccg cgagcgtgcc catacggatc agattgcgtc cttccattct
     2581 gtagtggctc tgcgtcgtgg ccaatgggta ctgctcgccg gtcttgcgga cctgcaagcc
     2641 cggggcggac cagggagccg cagcggtccg aatggcgtag gcattgaatc ccgtaccggt
     2701 ccccaccctg cccgcgcgcg ttcggccgta gccgaagtgc acggtgacgg aatccacggc
     2761 gtgccccggc atgatccaga cgggagcgcg caccgtccgc ccaccgaggt ccagctccac
     2821 cacgtcgccg gtggcgaggc tgagacgctc ggcggtcgcc gggctcagca gcgccgtgtt
     2881 gtcccaggtg agcttgctca ggggtttggg caattcctga agccagccgt tgttggcgaa
     2941 ccgtccatcc cagatggccg gatcggggcg gaacacgatc tccaggccgt cggcggattg
     3001 agccggtgcc gtcgccgccc cagcccccgg tcccgagacg ccaccccccg ccttcagact
     3061 gaccggccgc ggc
//
LOCUS       DBNGZC010000692         3144 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_646236, whole genome shotgun sequence.
ACCESSION   DBNGZC010000692 DBNGZC010000000
VERSION     DBNGZC010000692.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_646236"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            545..1510
                     /locus_tag="AC16G0_11915"
     CDS             545..1510
                     /locus_tag="AC16G0_11915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5100354.1"
                     /translation="MKRYWVVGVMVAAFLMAGPAFGAEPIKLGMSTWIGYAPLYLAKE
                     KGFFQKRGIEVEVVVIESPADRRAAFAADRLQGMATTVDTHVMTAAAENPIPVKQVLA
                     LDDSNGGDGMVAKKEIKTIKDLKGKTVAAQLGAGASYFWLNYVLAQNGMKLSDLKTVD
                     MKAGDAGAAFVAGKVDAAVTWEPWLSRAKETPFGSVLLSSDKTPGIIVDSLAFKPDFL
                     KKRGDDVKKLVAAWNEAVQFAAQNPNEADAIMAKFTNQKPEEFTKEKGGVRFYGEKEN
                     KEYFGTPQKPGLLYTVTQRAADLWSELKLIKTKPKATDLIDGSYL"
     gene            1614..2444
                     /locus_tag="AC16G0_11920"
     CDS             1614..2444
                     /locus_tag="AC16G0_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5100355.1"
                     /translation="MGEKNAEQSSKGNPRTIGDLLSPREPIPASLYLVLSLSSFALML
                     IVWSLLTYGGIVDALFLPSPGRVLRSGIDLFTELGFTTDILNSTYRVMLGFLIAAIIG
                     VPLGLVMGTFRVAEALTEPVVGFIRYMPASAFIPLFILWLGIDDVEKIAIIFVGSFFQ
                     LVLMVAVVAKNVPMDMLETAYTLGAGRFQVIRRVLLPASLPGILDTLRIIVGWAWTYI
                     IVAELVASASGIGYMIISAQRMLRTGSIIFGILTIGTMGLITDYFFKWLYKKLFPWAE
                     "
     gene            2450..>3144
                     /locus_tag="AC16G0_11925"
     CDS             2450..>3144
                     /locus_tag="AC16G0_11925"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5100356.1"
                     /translation="MADSIKLHIQNISKTYRSEGREVLAIDEASLEIRNKEFATILGP
                     SGCGKSTLLRIVAGLTKPTRGTVRLDGKMIGGPGQDRGMVFQTYTLFPWLTVKQNIRF
                     GLELSGVARAEQEQIAQEFIQKVGLKGFEEAYPKGLSGGMKQRVAIARALANNPAVLL
                     LDEPFGALDAQTRALMQELLTQVWEDLHKTILFVTHDVEEAVFLSDRVFVMTARPGKI
                     KAEIDIPLERPRS"
BASE COUNT          590 a         1000 c         1001 g          553 t
ORIGIN      
        1 ctgctccgtt cgcggggaag gccctcgccc ttgcagcgag aacaccgcaa gggctcagcc
       61 ctccccacgg acccttccga gaggggtggg ccgccacccc tcctcgcgct ctcccccgga
      121 ctgtgcggtc ctggaggggc tgttgcctat tttacgcacc gcacagtaat tccccattcg
      181 cattgcttct ccgccattgg gatggtacca tccgcccgct ccgccgaggt cccttctcaa
      241 tcagcgggga aggccgtcac gcggtctgcg gagacttggg aggctgctat tcgggtcctg
      301 ccgctgttgc atgcgattcc cacagccact cgaagcctta cggctcgtaa cgagccgcca
      361 actctccgct gaatcatcgc tcacgacacg ggacccaatg cgtgcgatca ccgtccgacc
      421 gaaggcctca cggacatgct gtcgaaaggg cacgctcagg cgggtgtgat gtaggatctc
      481 ttggcctgag aatccaagaa gcgctcgggg atgagggttg ttaaaggagg gagggacaat
      541 ggtaatgaaa aggtattggg tggtgggggt gatggtggcg gcattcctga tggccgggcc
      601 cgccttcggc gccgagccca tcaagctggg catgtccacc tggattggat acgccccgct
      661 gtacctagcc aaggagaagg gcttcttcca gaagcggggc atcgaggtcg aggtcgtggt
      721 catcgagagc ccggcggacc ggcgggcggc tttcgccgcc gacaggctcc agggcatggc
      781 gacgacggtg gatacccatg tgatgacggc ggccgccgaa aacccgattc cggtgaagca
      841 ggtgctggcc ctcgacgaca gtaacggcgg cgacgggatg gtggcaaaga aggagatcaa
      901 gacgatcaaa gacctgaagg ggaagacggt ggcggcccag ctcggtgccg gggccagcta
      961 tttctggctc aactatgtcc tggcccagaa cgggatgaag ctctccgacc tcaagacggt
     1021 ggacatgaag gcaggcgatg ccggggcggc ctttgtggcg ggcaaggtgg atgctgcggt
     1081 cacctgggag ccctggctct cccgagccaa ggagaccccg ttcggaagcg tcctcctctc
     1141 cagcgacaag acaccgggca tcatcgtgga ttccctggcc ttcaagcccg acttcctgaa
     1201 gaagcggggg gacgacgtca agaagctcgt ggccgcctgg aacgaggcag tccagttcgc
     1261 tgcgcagaat ccgaatgagg ccgacgccat catggcaaag ttcaccaacc agaagccgga
     1321 ggagttcacc aaggagaagg gcggcgtgcg attctacggc gagaaagaga acaaggaata
     1381 cttcgggacg ccgcagaagc ccggtctgct gtacaccgtg acccagcggg ccgcggatct
     1441 ctggtccgag ttgaagctca tcaagaccaa gcccaaggcg accgatctga tcgacggctc
     1501 gtacctgtaa ccgatccgcg aggttccatc cagccaatcc ggaaggcttg cgctgccggc
     1561 acgggcgggg tcgcccgtgc cggtgtctct cttgcctgac cgcctgggac ccgatgggtg
     1621 aaaagaacgc agaacagtcc tccaaaggca atccccggac gatcggagat ctcctgagtc
     1681 cgagggaacc catacccgcg agtctctacc tcgtcctcag cctcagcagc ttcgccctca
     1741 tgctcatcgt gtggtccctc ctcacctacg gggggatcgt ggacgccctg ttcctcccgt
     1801 cgccgggacg ggttctgcgg tcgggcatcg acctcttcac cgagctgggc ttcaccaccg
     1861 acatcctgaa cagcacgtac cgcgtgatgc tgggattcct catcgccgcc atcatcggcg
     1921 tgcccctggg cctcgtcatg gggaccttcc gggtcgccga ggcactgacg gagccggtgg
     1981 tcggcttcat ccgctacatg cccgcctcgg ccttcatccc tctgttcatc ctctggctgg
     2041 ggatcgacga tgtcgagaag atcgccatca tcttcgtggg gagcttcttc cagctcgtcc
     2101 tgatggtggc cgtcgtcgcc aagaacgtcc ccatggacat gctggagacg gcgtacaccc
     2161 tgggcgccgg acgttttcag gtcatccgca gggttctgct gccggcgagc ctgcccggga
     2221 tcctggacac gctgcggatc atcgtgggct gggcctggac ctacatcatc gtggcggagc
     2281 tggtcgcctc cgcctccggc atcggctaca tgatcatcag cgcccagcgg atgcttcgca
     2341 ccggaagcat catcttcggg atcctcacca tcgggacgat gggtctcatc accgactatt
     2401 tcttcaagtg gctctacaag aagctgttcc cctgggcgga gtgagacgcg tggccgactc
     2461 catcaagctc cacatccaga acatctccaa gacctaccgg tcggagggcc gcgaggtgct
     2521 ggccatcgat gaggccagcc tggagatccg gaacaaggag ttcgccacca tcctgggccc
     2581 ctcgggctgc ggcaagagca cgctcctccg gatcgtggcg ggcctcacga agccgacgcg
     2641 ggggacggtg cggctggacg ggaaaatgat cggcggcccc gggcaggacc gcgggatggt
     2701 cttccagacc tacaccctct tcccctggct caccgtgaag cagaacatcc ggtttggcct
     2761 ggagctgtcc ggcgtggcgc gggcagagca agagcagatc gctcaggagt tcatccagaa
     2821 ggtggggctc aagggattcg aggaggccta ccccaaaggg ctgtccggag ggatgaagca
     2881 gcgggtggcc atcgcccggg ccctggccaa caaccccgcc gtcctgctcc tggacgaacc
     2941 ctttggggcc ctcgacgccc agacgcgggc gctcatgcaa gagctcctca cgcaggtctg
     3001 ggaggacctg cacaagacca tcctcttcgt cacccacgac gtggaggagg ccgtcttcct
     3061 ctcggatcgg gtctttgtca tgaccgccag accggggaag atcaaagccg agattgacat
     3121 ccctttggag cggcccagaa gtta
//
LOCUS       DBNGZC010000693         5530 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2311619, whole genome shotgun sequence.
ACCESSION   DBNGZC010000693 DBNGZC010000000
VERSION     DBNGZC010000693.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2311619"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="AC16G0_11930"
     CDS             <1..372
                     /locus_tag="AC16G0_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR5100357.1"
                     /translation="RLHGDERILGESEFVEGVFKAAGERMERRHRLRVQGRDFDWLLR
                     RVAASVGLRSADLLTPSKVPARVEHRSLLCYWAVRELGLAGTIVAAKIGLTQSAVSRS
                     VARGELLVEEKAGITSKRIIS"
     gene            complement(447..1424)
                     /locus_tag="AC16G0_11935"
     CDS             complement(447..1424)
                     /locus_tag="AC16G0_11935"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0003714 - transcription corepressor
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_function: GO:0140297 - DNA-binding transcription factor
                     binding [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HIR5100358.1"
                     /translation="MGFIVIHTDPLHSDFSAEHQELSAIGAEFRTARCRTEAEVAVAC
                     KEAHGLIVTYAKVGRTALAAMPDLRIIVRTGVGYDTLDVPAATERKIMVANVPDYCVS
                     EVADHAMALSLSWWRRIGELDQQVRATGWGMPVKPVLRLEGRTLGILGLGRIGRAVAA
                     RARGFGLQVMASDPYVPQEVFASLGVRAASLEDLLRASDILTIHAPLTEETQGIIGDK
                     TLRLMKPSAVVVNTSRGGLIRTDDLVRAIREKRIAGAALDVIEVEPLPMDHPIRTLPR
                     VLLTPHAAWYSEDSEPELRRRAARIVAQALRGERPASLLNPEVLDQGRR"
     gene            1704..2618
                     /locus_tag="AC16G0_11940"
     CDS             1704..2618
                     /locus_tag="AC16G0_11940"
                     /inference="COORDINATES: protein motif:HMM:NF014615.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR5100359.1"
                     /translation="MGTQGQAFLDRLASEVLVLDGAMGTMLFAAGLSSGDCPELWNDA
                     RPDVLQKIHQAYYAAGSDLVETNTFGGTRLKLNAYGMQDRARELNLKAAQIARSVCPP
                     GKYVAGSMGPTGHLPDTFEPMGDTPAEVFYENFREQALALAEGGVDLLAVETMMIPEE
                     ATIAIKAAKEATGLPVMGSMTFQWGRGKNLDRTMWGTNPEAAARLLREAGADVVGCNC
                     GDGGPARVPFILREMQKAAPGFLVAYPNAGLPRIVDGRSVYDLSPEAMAADYLEILAA
                     GANVVGACCGSTPEHIRRIAEVVRGSRR"
     gene            complement(2676..2903)
                     /locus_tag="AC16G0_11945"
     CDS             complement(2676..2903)
                     /locus_tag="AC16G0_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HIR5100360.1"
                     /translation="MKISYDPRADALYIKFQEGSASRTRKVEEGLLVDLDENGRLYGI
                     EIIGMKGRIPIPELGKIILDFPIGGSEPARA"
     gene            complement(2900..3127)
                     /locus_tag="AC16G0_11950"
     CDS             complement(2900..3127)
                     /locus_tag="AC16G0_11950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HIR5100361.1"
                     /translation="MAVEFTNNALARMLDRGIGEADVRAALETPDHLGPSFEKRWRAK
                     KLVGKRTLEVVFFRDLKQTQVITTYWREPAP"
     gene            complement(3317..4093)
                     /locus_tag="AC16G0_11955"
     CDS             complement(3317..4093)
                     /locus_tag="AC16G0_11955"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5100362.1"
                     /translation="MSLERYVIFTPNRAIEATPQSEGLAFEDVWFSTSDGVRLHGWLV
                     PAPGADFTLLWFHGNMGNIGHCLNNLKYLHRSLRANLFIFDYRGYGRSEGRIGDLSEE
                     ATYRDADGAVACLRRRQDVSRTTLVYFGQSLGAAVAVDLARRQPAAGLVLETPFTCIR
                     DMARVIAPYWPFPSLLQTKYDSLRKIQEVRVPLLILHGDRDEWVPYEQGRRLFDAANE
                     PKTLYTIPGAHHNDTYVVGGEPYFEAWAGFLRTLGRTRVL"
     gene            4252..5106
                     /locus_tag="AC16G0_11960"
     CDS             4252..5106
                     /locus_tag="AC16G0_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017319448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB1 family protein"
                     /protein_id="HIR5100363.1"
                     /translation="MASHPVDPARPCRLAFARLVSRPEETIDLAEAALLIAQEEYPNL
                     DVAEYIGRLETMAAEVQAQVSTPVVPRRMIEVLNTYLFQAQGFRGNSEDYYDPRNSYL
                     NDVLDRRTGIPITLSTVYLAVGRRLGLPLHGVGMPGHFLVKYTGADEEVVIDPFNGGA
                     VVTPVDCQRLLDRIYDGKLAFEPRFLTTLGTRRILARMLSNLKVIHFNNHAYAKALTV
                     VDRLLILDPKAATEIRDRGLLSSQLKRFTQATADLERYLRLAPEAEDADVIRDHLRSM
                     RERVASMN"
     gene            5126..>5530
                     /locus_tag="AC16G0_11965"
     CDS             5126..>5530
                     /locus_tag="AC16G0_11965"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR5100364.1"
                     /translation="MKIYTRTGDRGETGLLGGGRVRKNALRVEAYGEVDELNATLGLC
                     SAALTDPDLLPLLLEIQRDLLAMGTQLADIRKEKATSTEKADFPGDKVVVLERAIDRF
                     EATLPPLRRFILQGGCEAGARLHLARTVCRRAE"
BASE COUNT          947 a         1809 c         1821 g          953 t
ORIGIN      
        1 cgcctccacg gggatgagcg gattctgggc gagagcgaat ttgtagaggg cgtttttaaa
       61 gctgctggtg agcggatgga gcggcggcac cgtctccgcg tgcaggggcg cgacttcgat
      121 tggcttctcc gtcgggtcgc cgcctcagtt ggcctccgat cggcggacct gttgacgccc
      181 agtaaagtcc cagcgcgtgt cgagcaccgc agcttgctct gctattgggc cgtgcgagaa
      241 ctgggcctcg ccggcacaat cgtggccgcc aagattggtc tcacccagtc ggccgtcagt
      301 cgatcggttg cgcgcggaga gcttctcgtc gaggagaagg cgggaatcac ttcgaaacgc
      361 ataatatcat gaccgtcccc tacgccctac gcatgctacg gatatccccc ggcgaagagg
      421 gtatcggtat tcgtcggtat tccgggtcag cgccttccct ggtccagcac ctcgggattc
      481 agcaaggagg cggggcgctc gccgcgcagg gcctgggcca cgatgcgggc tgcgcgccgg
      541 cggagctcgg gctccgaatc ctccgagtac caggccgcgt ggggtgtgag tagcacccgg
      601 gggagggtgc ggatggggtg gtccattggc agcggctcga cctcgatcac atccagggcc
      661 gccccggcaa tgcgtttctc ccggatggcg cggacgaggt cgtcggtacg gatgagcccc
      721 ccgcgcgagg tgttcacgac gacggccgag ggcttcatga ggcggagagt cttgtcgccg
      781 atgatcccct gcgtctcctc cgtaaggggg gcgtggatgg tcaggatatc cgaggcccgc
      841 aggaggtctt cgagacttgc cgcccggacg cccagggagg cgaacacctc ctgcggcacg
      901 tagggatcag aggccatcac ctgcagcccg aatcctcgcg cccgcgcggc cacggcccgc
      961 ccgatccgcc ctaagcccag gatgcccagc gtccgcccct ccagccgcag caccggtttc
     1021 acgggcatcc cccagcccgt cgcccgcacc tgctggtcca gctccccgat ccgccgccac
     1081 caggagaggg agagggccat ggcatggtcg gccacctcgc tcacgcaata gtccggcacg
     1141 ttggcgacca tgatcttgcg ctccgtggcc gcaggcacgt ccagcgtgtc gtaccccacg
     1201 ccggtccgga cgatgatgcg gaggtccggc atggcggcca gggcggtgcg gcccaccttg
     1261 gcgtaggtga cgatgaggcc gtgggcctcc ttgcaggcta ccgccacctc tgcttcggtg
     1321 cgacaccggg cggtgcgaaa ctccgccccg atggcagaga gttcctggtg ttcggcgctg
     1381 aagtccgagt ggagcgggtc cgtgtggatg acgataaacc ccatgggaga cctccgtcgg
     1441 atttctgtgg gtcgggtgga aggttcaaga tcctgtcgcg gtaccgcggg cgccgtcggt
     1501 tcggcagcga cagggagagt gcctgccccg cgctgaccgg tgcatcgccc gcacacttgt
     1561 atggcttggc gccgggacat gcaaggcaaa acttcgcatc gtccggcaga cccggttttc
     1621 ccttgtcacg tcgctgccct ttgggataaa aggaacggag cgccatgaca cgccatcgaa
     1681 cgacccacgg gaggaaaaat cgtatgggaa cccaaggtca ggcgttcctg gatcggctcg
     1741 catccgaggt gctggtgctc gacggggcca tgggcaccat gctcttcgcg gcgggcctca
     1801 gtagcggaga ctgtccggag ctgtggaacg acgcccgtcc cgacgtgctc cagaagatcc
     1861 accaggccta ctatgcggcg ggcagcgacc tcgtggagac caacaccttc ggcgggaccc
     1921 ggctcaagct gaacgcctac gggatgcagg atcgggctcg cgagctgaac ctcaaggcgg
     1981 cgcaaatcgc acgctcggtc tgtcctccgg gcaagtacgt cgccggctcc atgggaccca
     2041 cggggcatct ccccgacacc ttcgagccca tgggcgacac acccgcggag gtgttctacg
     2101 agaacttccg ggagcaggcc ttggccctgg ccgaaggggg cgtggacctg cttgccgtcg
     2161 agaccatgat gatcccggag gaggcgacca tcgccatcaa ggccgccaag gaggcaaccg
     2221 gtcttcccgt gatgggcagc atgacctttc agtggggcag gggaaagaac ctggaccgga
     2281 ccatgtgggg aaccaatccg gaggcggcgg ctcggctgct tcgcgaggcc ggcgccgacg
     2341 ttgtggggtg caactgcggg gatggcgggc cggcccgcgt gcccttcatc ctgcgcgaga
     2401 tgcagaaggc cgccccggga tttctggtgg cttacccgaa tgccggtctg ccgcggatcg
     2461 tggacggccg ttcggtctac gatctatcgc ccgaggcaat ggcggccgac tatctggaaa
     2521 tcctcgcggc cggggccaac gtcgtcggcg cctgctgcgg cagcacgccc gagcacatcc
     2581 ggcggatcgc ggaggtggtc cgggggagcc ggaggtgacc acgctgcacc tcgtctgcaa
     2641 cgtgcgcaga ttcctcgagg gacctgatgc gatgattacg cccgggcggg ctcggaccct
     2701 ccgatgggga agtccaggat gatctttccc agctcgggaa tggggatacg ccccttcatg
     2761 ccgatgattt caatgccgta gagacggccg ttctcgtcca agtccaccaa cagcccctcc
     2821 tccactttcc gcgtccggct cgcgctcccc tcctggaact tgatgtacag ggcatcggct
     2881 cggggatcgt agctgatctt catggggccg gctccctcca gtacgtcgtg atgacctgcg
     2941 tctgcttcag gtcccggaag aagaccacct ccagcgtgcg cttccctacc aacttcttgg
     3001 cgcgccagcg cttctcgaag ctggggccga gatggtccgg ggtctccagc gccgcccgga
     3061 cgtcggcctc tccgataccc cggtccagca tccgggccag cgcgttattt gtgaattcta
     3121 ccgccatcgc atctcccgac aaccctcctc cagcccttcc caacgggcac ccagcgtgtc
     3181 gctcacacgg gtcgaggcac ggaagctctt ccggggatcc ttggttcggt catcccgccc
     3241 gcctgggcgg cctgtctcgc cctttgaccg gggcggaaaa gccgaacctc acctttgcca
     3301 gcgcctgctt ggtatgttat aaaacacgcg ttcgccccag ggtgcgcagg aacccggccc
     3361 acgcctcgaa gtatggctcg cctcccacaa cgtaggtgtc gttgtggtgg gcgccgggga
     3421 ttgtgtaaag ggtcttgggc tcgttggcgg cgtcgaagag acggcgccct tgctcgtagg
     3481 ggacccactc gtcacgatcg ccgtgcagga tcaacagcgg gacccgaacc tcctgaattt
     3541 tcctcagcga atcgtacttg gtttgcagca agctcggaaa cggccagtag ggtgcaatga
     3601 cccgggccat atcgcggatg caagtgaagg gcgtctccaa caccagaccc gcggccggct
     3661 ggcgccgggc gagatccacg gcgacggccg cccccaggga ttgtccgaaa tagaccagcg
     3721 tggtgcggga cacgtcctgc cgtctccgga ggcaggcgac ggccccgtcg gcatcgcggt
     3781 acgtcgcctc ttccgagaga tcgcctatcc tgccctcgct cctgccatag ccgcggtagt
     3841 cgaatataaa gaggttcgct ctcagtgatc ggtgcaggta tttgaggttg ttgaggcagt
     3901 ggccgatgtt ccccatgttt ccgtggaacc agagaagcgt gaaatctgcg cccggcgccg
     3961 ggaccagcca gccgtgaagc ctgacgccgt cgctcgtgga gaaccacacg tcctcgaagg
     4021 cgagtccctc gctctggggg gtcgcctcga tggcgcggtt tggtgtgaat atgacgtatc
     4081 tctccaaact caagatcatt ctcatcccca atacggccgc ccagaggagc gggacacgct
     4141 agaccaaggt ggtggtggcg gaagcctgga aactgcgtca agaatgccag agccggccgc
     4201 ccacggcaag tgataaacgt cgcggcggcg tggagccgcc gatggaggac catggcatcg
     4261 catcctgttg accctgcccg tccctgccgt ctggctttcg ccagactcgt cagtcgtccc
     4321 gaagagacga tcgacctggc ggaagcggcg cttctgatcg cccaggagga gtatcccaac
     4381 ctggacgtgg ccgaatacat cggtcgtctc gagaccatgg ccgccgaggt ccaggcccag
     4441 gtgagcactc cggtggtacc ccgtcgcatg atcgaggtcc tcaacaccta tctgttccag
     4501 gcccaggggt tccgcggcaa cagtgaggac tactacgacc cgcgcaacag ctatctgaac
     4561 gatgtcctgg accggcggac cggcatcccc atcaccctct ccaccgtcta tctggccgtg
     4621 ggaaggcgcc tgggcctgcc gttgcatggg gtgggcatgc ccggtcattt cttggtgaaa
     4681 tacacgggcg cagacgaaga ggtcgtgatc gaccctttca acgggggcgc ggtcgtgacg
     4741 cccgtcgatt gccagcggct gctggatcgg atctacgacg ggaagcttgc cttcgagccg
     4801 cgcttcctga cgaccttggg gacccgccgg atcctggccc ggatgctctc caacctcaaa
     4861 gtcatccact tcaacaatca cgcctacgcc aaggccctga ccgtcgtgga tcgcctgctg
     4921 atcctggatc ccaaggccgc cacggagatc cgtgatcggg ggttgctctc ctcgcagctc
     4981 aagcggttca cccaggccac agccgatctg gaacggtacc tgcggctggc gccggaggcc
     5041 gaggatgccg acgtgatccg ggaccatttg cgctccatgc gcgagcgcgt ggcctcgatg
     5101 aactgagcgt gatccgggaa gaccaatgaa gatctacacg aggacaggcg atcgaggaga
     5161 gacggggctg ctcggcgggg gacgcgtgcg gaagaacgcc ctgcgggtcg aggcctacgg
     5221 cgaggtggac gaactcaacg cgaccctcgg gctctgctct gcggccctca cagacccaga
     5281 cctgctcccc ctcctcctcg agatccagcg ggacctgctg gccatgggga cgcagcttgc
     5341 ggatatccgc aaggagaagg ccacgagcac ggaaaaggcc gatttccctg gagacaaggt
     5401 ggtcgtgctg gaacgcgcca tcgatcgctt cgaggcgacg ctcccgcccc tgcggcggtt
     5461 catcctgcag ggcgggtgcg aggccggcgc ccgcctccac ctggcccgaa ccgtctgccg
     5521 gcgggccgag
//
LOCUS       DBNGZC010000694         6908 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2604655, whole genome shotgun sequence.
ACCESSION   DBNGZC010000694 DBNGZC010000000
VERSION     DBNGZC010000694.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6908)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2604655"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1908
                     /gene="topA"
                     /locus_tag="AC16G0_11970"
     CDS             <1..1908
                     /gene="topA"
                     /locus_tag="AC16G0_11970"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR5100365.1"
                     /translation="DGVGSAFPARLLRVDGEKAAVPSQTDADRILAELSAPGTLFRVA
                     SLVKKEKRRNPVPPFTTSKLQLEAARKLHFPVRKTMQVAQQLYEGIEVGDAGAVGLIT
                     YMRTDSTRISPEAQAEVRQFIGDRYGQAFLPERAPTYRSGKGAQEAHEAIRPTSVLRD
                     PAQVAPYLTREQLALYTLIWNRFVASQMRPAVFDATTVDISAGRFGFRASGQHMKFAG
                     FMQVYVEGQDEAARGTPGEAEADEDEERSLPPLEEGQTLDCRALTPTQHFTQPPFRYS
                     EASLVKELEERGIGRPSTYAAILSVIQNRDYVVKDQGKFKPTELGEIVVDLLIESFPR
                     ILDPGFTAQMETKLDEIEEGTADWLEAMHKFYGAFTRWLEQAKHKMKNVKAMEEPTDI
                     ACEKCGRPMVIKWGRFGKFLACSGYPECKSTKELPGNGEGSAVAGTNGQGEAVGEGQA
                     CENCGKPMVLKRGRFGPFLACSGYPECKTIVRIGRAAAPPPKPTDQVCEKCGAPMVIR
                     EGRFGPFYSCSTYPTCKNLKPIPIGINCPECGAPLTARRTKRGRTFYGCSAYPKCDFT
                     LWDRPVPEPCPACHAPFLVEKRLKSGVKLRCIKEGCEYERDAEPPAQPTAADPESSDG
                     GAAPAVSVPQS"
     gene            1974..3404
                     /gene="trmFO"
                     /locus_tag="AC16G0_11975"
     CDS             1974..3404
                     /gene="trmFO"
                     /locus_tag="AC16G0_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HIR5100366.1"
                     /translation="MSDEFVITVIGGGLAGCEAAWRAARLGASVRLFEMRPRSMTGAH
                     QTDRLAELVCSNSLKSESLEDASGLLKAEMRALDSLILRWAETHRVPAGSALAVDRDA
                     FASAITQAITDEGRIQVVRQEVPEIPPDRPVIVATGPLTSDRLSADLAARFAAISEPR
                     AASREPRAENLEPHTSNRERRPSSLLYFYDAISPIVSADSVNRDVAFAAGRYEKGGAD
                     YLNCALTREEYLAFREALLLGERYPLHAFEAPKYFEGCLPIEELAERGEDTLRFGPMR
                     PVGLKDPRTGHRPYAVVQLRAENLGPDGSPSMYNLVGFQTRLRRGDQARALRLIPGLQ
                     QAEILRYGSVHRNTFLAAPALLQETLQFQGDTEIFFAGQLTGVEGYLESAATGILAGI
                     NAVRVSRGEGPVCPPPTTMLGALLRYITKADPRTFQPINANFGLLPPLPQIVRKREER
                     NRLLVERALADFRVCQAGLQSSSPGN"
     gene            3464..3694
                     /locus_tag="AC16G0_11980"
     CDS             3464..3694
                     /locus_tag="AC16G0_11980"
                     /inference="COORDINATES: protein motif:HMM:NF021560.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HIR5100367.1"
                     /translation="MRIEYSKGVDALYIHLRDVPVAESRDVEEGVTLDFDAEGHVVGL
                     EILDASDRLGLSNLVNVSIENLPVDRVPFEAA"
     gene            3880..4209
                     /locus_tag="AC16G0_11985"
     CDS             3880..4209
                     /locus_tag="AC16G0_11985"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR5100368.1"
                     /translation="MGQLAIADPKLAEIVRRLVEAYHPERIYLFGSRARGDSSPDSDY
                     DLMVVVPDSTPPQRRRSRLAYEVLWEAGSGADVLVWTRNAFESRLHLRASLPATVIRE
                     GRLLHAA"
     gene            4199..4612
                     /locus_tag="AC16G0_11990"
     CDS             4199..4612
                     /locus_tag="AC16G0_11990"
                     /inference="COORDINATES: protein motif:HMM:NF017020.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HIR5100369.1"
                     /translation="MRHDPGRVADTRAWLVKAANDLRAAETDVAASPPLLEDALFHCQ
                     QAAEKAMKALLTWHDEPFRKTHDLVEVGRQCVEIDATLESLLRRSAPLTEYAWKFRYP
                     GDSEEPSRDEAEEAMTLARQVYEAVLGRLPGEVHP"
     gene            4693..5145
                     /locus_tag="AC16G0_11995"
     CDS             4693..5145
                     /locus_tag="AC16G0_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK family nuclease"
                     /protein_id="HIR5100370.1"
                     /translation="MNRYKPIGTTAEVEFKTIRPCYMTARSHINQVVADTLAWEGSAA
                     FRLEQSAAAACYARNEHLGLVIPYEFMGVDYSYEPDFLVRLANGLTLILDVKGFEDEQ
                     TRAKHEAAKRWVMAENNWGQLGKWMFHVCRNPLMLDKELAYLLHESQE"
     gene            5296..5613
                     /locus_tag="AC16G0_12000"
     CDS             5296..5613
                     /locus_tag="AC16G0_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HIR5100371.1"
                     /translation="MGGFPDELSECTGFHWDAGNVDKNWDLHQVSQAECEQVFFNRPI
                     LVASDEKHSQGEPLYAAFGQTNAGRRLAVIFTVRGLLLRVISAQDMSRRERRVYEAVS
                     EVE"
     gene            5588..5887
                     /locus_tag="AC16G0_12005"
     CDS             5588..5887
                     /locus_tag="AC16G0_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HIR5100372.1"
                     /translation="MKQSAKSSKKPIPRFASEDAERRFWAEHDSTDYFDWSKTARPSF
                     PNLKPSTTSISIRLPLSMLDELKALANEKDVPYQSLMKVYLAERLARERGRKIPS"
     gene            6039..>6908
                     /locus_tag="AC16G0_12010"
     CDS             6039..>6908
                     /locus_tag="AC16G0_12010"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR5100373.1"
                     /translation="MAGCRVCRLGAWRLAVSIFGGAVREAIEQYLTYLTAERDASVHT
                     LRNYRVDLSQFLAYLRERQDDRSDPDPANVDPLAVRGFLARLHGAGLTKTSMARKLAT
                     LRSFFRFLCREGRLRTNPAELVQSPRLPVRSTPHLSVDEAFQLLAAPATAPRPPTRIS
                     AILRVRDRAILELFYASGLRIGELVGLNRSDVDLQEGLVRVLGKGRKERIVPVGKKAI
                     EALNAYLAGCEALTPGSTDRKEGPQGLFLNHRGGRLTSRSVSRTVLRHLLASGLGKKV
                     TPHGLRHSFATHLL"
BASE COUNT         1237 a         2207 c         2243 g         1221 t
ORIGIN      
        1 gatggtgtcg ggtcggcatt ccccgcccgt ctccttcggg tggacgggga gaaagccgcc
       61 gtcccatcgc agacggacgc cgaccggatc ctggccgagc tgtccgcccc cggcaccctg
      121 ttccgggtgg ccagcctggt caagaaggag aagcgcagga accccgtccc gcctttcacc
      181 accagcaagc tgcagctgga ggcggcgcgg aagctccact tcccggtgcg caagaccatg
      241 caggtggcgc agcagcttta cgaggggatc gaggtggggg acgcgggagc cgtgggtctc
      301 atcacctaca tgcgcaccga ctccactcgc atctcgcccg aggcccaggc ggaggtccgg
      361 caattcatcg gggaccgcta cggccaggcc ttcctccccg agcgggcccc cacgtaccgg
      421 agcgggaagg gagcccagga ggcccacgag gccatccgcc cgacctcggt tctgcgtgac
      481 ccggcccagg tggcgcccta tctcacccgg gagcaactgg ccctctacac gctgatctgg
      541 aaccgattcg tggcctccca gatgcgcccc gcggtcttcg acgcgacgac ggtggacatc
      601 tccgcgggcc gcttcggctt ccgggccagc ggccagcata tgaagtttgc cggcttcatg
      661 caggtgtacg tggagggaca ggacgaggcc gctcggggca cacccgggga ggcggaggcc
      721 gacgaggacg aggagcgaag cctgccgccg ctcgaggagg gccagaccct cgattgccgg
      781 gccctcaccc cgactcagca cttcacgcaa ccgcccttcc gttacagcga ggcttccctg
      841 gtcaaggaac tggaggagcg aggcattggt cgccccagca cctacgccgc catcctgagc
      901 gtgatccaga accgggacta cgtggtgaag gaccagggga aattcaagcc cacggagctg
      961 ggcgagatcg tcgtggacct cctcatcgag agcttccccc gcatcctgga ccctggcttc
     1021 accgcccaga tggagacgaa gctggacgag atcgaggagg gaacggccga ctggctggag
     1081 gccatgcaca agttctacgg ggcctttacc aggtggctgg agcaggccaa gcacaagatg
     1141 aagaacgtca aggccatgga ggagccgacc gatatcgcct gcgaaaaatg cgggcgcccc
     1201 atggtcatca agtgggggcg gttcgggaag ttcctggcgt gctcgggcta cccggagtgc
     1261 aagagcacga aggaactccc cggaaacggg gagggcagcg cggtcgccgg gacaaacggt
     1321 cagggcgagg cggtgggtga gggccaggcc tgcgagaatt gcggaaagcc catggtcttg
     1381 aagcgcggcc gcttcggacc attcctggcc tgctcgggat accccgaatg caagacgata
     1441 gtccggatcg gccgggccgc ggcgccgccg ccgaagccga cggatcaagt ctgcgagaag
     1501 tgcggggcgc ccatggtcat ccgcgagggg cggttcggcc ccttctactc ctgcagcacg
     1561 tatccgacgt gcaagaatct gaaaccgatc cccatcggga tcaactgccc agagtgcggc
     1621 gcgcccctca cggctcggcg gaccaagcga ggccggacgt tttacgggtg cagcgcctat
     1681 cccaagtgcg acttcaccct ctgggaccgc ccggtccccg agccctgtcc ggcctgtcac
     1741 gccccgtttc tggtggagaa gcggttgaag tcgggcgtga agcttcgctg catcaaggag
     1801 gggtgcgagt acgagcgcga cgccgagccc ccggcgcagc cgacggcggc cgacccggaa
     1861 tccagcgatg gtggcgcagc ccctgccgtc tcggtccccc aaagctgacg gccgatcgac
     1921 ccatcgctca gggtgggcgc cgtaagccat acaccgcctc ttccagtgtc agcatgtcgg
     1981 atgaattcgt gatcaccgtg atcgggggcg gcctggcggg ctgtgaggcg gcctggcgcg
     2041 ctgcgcgtct cggcgccagc gtccgcctgt tcgagatgcg accccggtcc atgaccgggg
     2101 cgcaccagac cgacaggctc gccgagctgg tctgcagcaa ctccctgaag tccgagtccc
     2161 tggaagacgc cagcggcctg ctcaaggcgg agatgcgggc gctggactcc ctgatcctgc
     2221 gttgggcaga gacgcaccgg gtcccggccg ggtcggcact ggccgtggac cgggacgcct
     2281 tcgcctcagc gatcacccaa gccatcactg acgagggccg aatccaggtt gtccgccagg
     2341 aggttccgga gatccccccg gaccggcctg tgatcgtggc caccggcccg ctgacctcgg
     2401 atcgcctttc tgcggatctc gccgcgcgat tcgccgcaat ttccgagccg cgagccgcga
     2461 gccgcgagcc gcgagcggag aacctcgaac cgcacacgtc gaaccgcgaa cgtcgcccgt
     2521 cgagcctcct ctacttctac gacgccatct cgcccatcgt ctccgccgac tcggtgaacc
     2581 gggacgtcgc cttcgccgcg gggcggtacg agaagggggg ggccgattac ctcaactgcg
     2641 cgctgacgcg ggaagagtac ctggcattcc gggaagcgct gctcttgggc gagcggtatc
     2701 ccctgcacgc cttcgaggcg ccgaagtact tcgaagggtg cctgcccatc gaggagctgg
     2761 cagagcgggg agaggacacg ctccgcttcg ggccgatgcg tccggtgggg ctcaaggatc
     2821 ctcgcaccgg ccatcggccc tacgcggtcg ttcagctccg ggcggaaaac ctggggccgg
     2881 acggctctcc cagcatgtac aacctcgtgg gcttccagac ccgcttgcgg cgaggcgatc
     2941 aggcccgggc gctccgcctg atccccggct tgcagcaggc ggagatcctt cgctacggca
     3001 gtgtccaccg gaacacgttc ctggcggccc ccgccctgct ccaagagacg ctgcagttcc
     3061 agggggacac ggagatcttc ttcgccgggc agcttaccgg agtcgagggg tatctggagt
     3121 ccgcagccac gggaatcctg gccgggatca acgccgtccg cgtctcccgg ggcgagggcc
     3181 ccgtttgccc tcctcccacg accatgctgg gcgcactcct gcggtacatc accaaagccg
     3241 acccccggac cttccagccc atcaacgcca acttcggtct cttgcccccg ttgccgcaga
     3301 tcgtccgcaa gcgcgaggag cgcaaccgcc tcctggtcga gcgcgccctg gcggatttcc
     3361 gggtgtgtca ggcgggtctc caatcatcaa gtccaggcaa ttgaccggtg actggtattc
     3421 cacacagtag ttcggctaaa cagttctcta ggaggggtaa gccgtgagga tcgagtacag
     3481 caagggcgtg gatgccctgt atattcatct ccgggatgtg ccggtcgcgg aaagccgaga
     3541 tgtcgaggag ggagtcacat tggacttcga tgcggaggga catgtcgtcg gtctcgagat
     3601 tctggatgcc tcagatcggc taggactaag taacctcgtc aacgtctcca ttgagaacct
     3661 cccggttgat cgcgttccct tcgaagccgc gtagattcca cctcagcccg gcccttttct
     3721 cctatcgtct cttgggtgcg ccccgctccc cccgggcgtg tcctggccct tgcatcccag
     3781 ggtggcattt ggtacaaagt caacaatccc tcttggtttt taggcgccca gcgcgcaaga
     3841 accaggcggg atgcttcgag tcggttaagg aagatcgcca tgggccagct tgcgatcgca
     3901 gatccgaagc tagccgaaat cgtccgacgg ctggtagagg cctatcaccc tgagcggata
     3961 tacctctttg gctcccgcgc tcggggggac tcgtctcctg acagcgatta tgatttgatg
     4021 gttgtggttc ccgattcgac acccccacag cgacgacgga gccgcctcgc atacgaagtc
     4081 ctctgggaag ccggttcggg tgccgatgtc cttgtctgga cccgcaatgc ttttgagagc
     4141 cggctccatc tgagggcgtc gctgccggcc acggtcattc gggaaggcag gttgctccat
     4201 gcggcatgat ccaggccggg tggcggacac ccgtgcgtgg cttgtgaaag ccgcgaatga
     4261 tcttcgtgct gcagaaaccg atgttgctgc gtcacctccc ttactggagg acgcactctt
     4321 ccattgtcag caagcagcgg agaaggcgat gaaggcactt ctcacctggc acgatgagcc
     4381 ctttcggaag acgcacgatc tggttgaggt tggaagacag tgcgtcgaga tagatgcgac
     4441 actggaatcg cttcttcggc gctcggcgcc actcacggaa tatgcctgga aatttcgata
     4501 cccgggcgac tctgaggagc cttcgcgaga cgaagcggag gaggccatga ctcttgccag
     4561 gcaagtctat gaagccgttc ttgggcgctt gccaggggaa gttcatccgt aggaggggcc
     4621 gtcgcggcga aggggctgcg agatgccatc gtccctgacg aggccgaggg ggagccaccg
     4681 ctcctgccgg tcttgaaccg gtacaagccc atcggcacga cggccgaggt ggagttcaaa
     4741 acgatacgcc cctgctacat gaccgcacgg agccatatca atcaagtcgt ggcggacacg
     4801 ctcgcctggg aagggagcgc ggcgtttcgc ttggagcaga gtgcggcagc ggcgtgctac
     4861 gccaggaacg aacatctggg cttggtcatc ccctatgagt tcatgggtgt tgattacagc
     4921 tacgagcccg acttcctggt tcggctggcc aacggcctaa cgctgatttt ggatgtcaag
     4981 ggatttgagg acgagcagac ccgggcgaag catgaggcgg ccaagcggtg ggtgatggcc
     5041 gagaacaact gggggcagct cgggaaatgg atgttccacg tatgtcgcaa tccactaatg
     5101 ctggataaag aactggccta tcttcttcac gagagccagg agtaagcggt cacgacattc
     5161 tcttcaacgg ttctgtggga ggcgtgccct gcagtgcttg aactccagct tttccgagtg
     5221 aagatccttc ccgctgcagg tcggcccacc attccgtccg ttgacccgcg tatatcctta
     5281 tgatatacaa ctcccatggg aggcttccct gacgaactgt cggaatgcac cgggttccat
     5341 tgggacgccg gtaacgtcga caagaactgg gatctccacc aggtctcaca ggccgaatgc
     5401 gaacaggtat tcttcaaccg gccgatcctg gtcgcatccg atgaaaagca ctcacagggc
     5461 gagcctctgt acgctgcatt tgggcaaacc aatgccggcc gccgactggc ggtgatattc
     5521 actgtccgcg gcctgctgtt gcgcgtcatc tccgcccaag atatgagccg gcgagagcgg
     5581 agggtttatg aagcagtcag cgaagtcgag taagaaaccg atccctcggt ttgcctccga
     5641 ggacgccgag cggcggttct gggccgagca cgattccacc gactacttcg actggagtaa
     5701 gacggctcgg ccatcgtttc ccaacctgaa gccatcgacc acctcgatct ccatcaggct
     5761 cccgctttct atgctcgatg agctgaaagc gctcgcgaat gaaaaggacg taccatacca
     5821 gtcgctcatg aaggtctatc ttgccgagcg cttggcgcgg gagcgggggc gaaagattcc
     5881 ctcctgatgc ccgcagtgag cctggacgcc tgtcgcatgg tgcggtctgg ctactccgcc
     5941 gactcctcct gacccgcttc ctaccgccct gggccagatg atatgatggc tgcttttcgc
     6001 ttgatctttg gggcgctctg gcgtagattc cgcgcggagt ggccggttgc cgggtctgcc
     6061 gcctgggggc ctggcggctg gccgtttcga tttttggggg agccgtgcgc gaggccatcg
     6121 agcagtacct gacgtatctt actgccgaac gtgacgcctc ggtccacacc ctgcggaact
     6181 accgggtgga cctcagccag ttcctggcgt atctccgcga gcggcaagac gacaggtcgg
     6241 atcccgatcc agcgaacgtc gatccgctcg ccgtgcgggg attcctggct cgactccacg
     6301 gggcgggctt gaccaagacc agtatggccc ggaagctggc cacgctccgg tcgttcttcc
     6361 ggttcctctg ccgggagggg cgtcttcgga cgaaccctgc agaactggtg cagagtcctc
     6421 gtctgccggt gcggtcgacg ccccatcttt ccgtggacga ggccttccag ttgctggccg
     6481 cgccggcgac cgctccgagg cctcccacga ggatcagcgc aattcttcgc gtgcgcgatc
     6541 gggcgatcct ggagctgttt tacgcctcgg gtcttcgaat cggggagctg gtgggactga
     6601 accggtcgga cgtggatctc caggaggggc tggttcgggt cctggggaag ggccggaagg
     6661 agcgcatcgt tccggtgggg aagaaggcga tcgaggccct caatgcctat ctcgctgggt
     6721 gtgaagctct gacgcccgga agcacggatc ggaaggaagg gcctcagggc ctcttcctga
     6781 accatcgcgg agggcgcctc acgtcccgga gcgtctccag aaccgtcctc cggcatctgc
     6841 tggcgagcgg tctgggaaag aaggtcacgc cccacggcct gcgccacagc ttcgccaccc
     6901 acctactg
//
LOCUS       DBNGZC010000695         1733 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1527315, whole genome shotgun sequence.
ACCESSION   DBNGZC010000695 DBNGZC010000000
VERSION     DBNGZC010000695.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1527315"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /gene="nrdR"
                     /locus_tag="AC16G0_12015"
     CDS             complement(<1..355)
                     /gene="nrdR"
                     /locus_tag="AC16G0_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR5100374.1"
                     /translation="MAEKVVDSREGKDGGVIRRRRECLQCHRRFTTSERIEEIHFMVV
                     KKDGRREPFDRHKLLSGLLKATQKRPVSVAQLEEIVDDIEGRLSEKPDREIASSEVGE
                     LIMQRLHDLDEVAYVR"
     gene            complement(415..1665)
                     /locus_tag="AC16G0_12020"
     CDS             complement(415..1665)
                     /locus_tag="AC16G0_12020"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR5100375.1"
                     /translation="MKRLIDVDPEIAKVIRLETERQASKLELIASENFVSEAVLEAMG
                     SPLTNKYAEGYPGKRYYGGCEFVDMAESLAIERAKQLFGADHVNVQPHAGAQANMSAY
                     FAVLNPGDTILGLNLAHGGHLTHGSPVNFSGRFFKVIPYGVNRETEQIDMAEVRRLAQ
                     EHRPKLIVIGGSAYPRTLQFDQFRVIADEIGCLLMADIAHPAGIIAAGLHPSPIPHCE
                     LVTTTTHKTLRGPRGGMIMCKAAYAAAVNKNIFPGMQGGPLMHVIAAKAVAFKEALTP
                     EFRLYEQQIVANAKALAEALISHGFRLVSGGTDTHLMLVDLRPKGLTGKAAETALDRA
                     GMTVNKNAIPFDDKPPTVTSGIRVGTPALTTRGMREAEMRQVAALMAEVLADIEDVNR
                     QASVAARVRELCAAFPLYRERLTP"
BASE COUNT          275 a          574 c          554 g          330 t
ORIGIN      
        1 accggacgta agccacctcg tccagatcgt gcagacgctg catgatcagc tcgcccacct
       61 cgctggaagc gatttcccgg tccggcttct ccgagagacg cccctcgata tcgtccacga
      121 tctcttcgag ttgggccaca ctcaccggcc gcttctgcgt cgccttcagc aggccgctca
      181 gcagcttgtg tcgatcgaag ggttcgcggc gaccgtcctt cttcaccacc atgaagtgga
      241 tctcctcgat ccgctcagag gtggtgaatc ggcggtggca ctgcaggcac tcgcgccgac
      301 ggcggatgac cccgccatcc ttcccttcgc gggagtccac taccttctcc gccatggtgc
      361 tgcagtacgg acagcgcacc gagattgctc cggtccccga cgcgagggga agggttaggg
      421 ggtcagacgc tcccggtaca gggggaaggc ggcgcacagt tctcgcaccc gggccgccac
      481 cgaggcctgg cggttcacat cctcgatatc tgccaagacc tcggccatca gggccgccac
      541 ctgacgcatc tctgcctcgc gcatgccgcg cgtcgtaagt gcgggcgtgc caacccggat
      601 gcccgaggtg accgtggggg gcttgtcgtc gaaggggatg gcgttcttgt tcaccgtcat
      661 gccggcccga tccagggctg tctcggccgc cttgcctgtg agccccttgg gcctgagatc
      721 caccagcatc aggtgggtgt cggtgccgcc cgagacgagc cgaaacccgt gggagatgag
      781 cgcctcggcc agcgccttgg cattcgccac gatctgctgt tcgtagaggc ggaactccgg
      841 cgtgagggct tccttgaagg ccaccgcctt ggccgcgatg acgtgcatca ggggaccgcc
      901 ctgcattccg gggaagatgt tcttgttcac cgccgccgcg taggcggcct tgcacatgat
      961 catgccgccc cgggggcccc gcagcgtctt gtgcgtcgtg gtcgtgacca gctcgcagtg
     1021 agggatgggc gaggggtgga gtcccgccgc gatgatccct gcgggatggg caatgtccgc
     1081 catgagcagg cacccgatct catcggcgat cacgcggaat tggtcaaact ggagcgtacg
     1141 cgggtaggcg ctgccgccga tcacgatgag ctttggccgg tgttcctggg cgaggcgccg
     1201 gacctcggcc atgtcgatct gctcggtttc ccgattcacc ccgtagggga tgaccttgaa
     1261 gaatcggccg gagaagttga ccggactccc gtgggtgagg tggccgccgt gcgccaggtt
     1321 cagcccgagg atcgtgtcgc cggggttcag gacggcgaag tacgcggaca tgttggcctg
     1381 ggccccggcg tgcggctgca cgttcacgtg gtcagctccg aagagctgct tggcgcgctc
     1441 gatggccagg ctctccgcca tgtccacgaa ttcgcaaccg ccgtagtacc gcttccccgg
     1501 atatccttcg gcgtatttgt tggtcaaggg ggagcccatc gcctccagca cggcttcgct
     1561 gacgaagttt tccgaggcaa tcagctccag cttggaggcc tgccgttccg tctccagtcg
     1621 aatgaccttg gcaatctccg gatccacatc aatcaatcgc ttcacggccg ctcccctccc
     1681 tccacaacgc acgaatccag gtccgtccag tcggaaatag gagtctagcc tcg
//
LOCUS       DBNGZC010000696         2183 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2311675, whole genome shotgun sequence.
ACCESSION   DBNGZC010000696 DBNGZC010000000
VERSION     DBNGZC010000696.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2311675"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="AC16G0_12025"
     CDS             complement(<1..845)
                     /locus_tag="AC16G0_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HIR5100376.1"
                     /translation="MTQHRLEVADVFREHGHAFLEQYGDTLSPERRRALRDIAACRTA
                     ALGGHVEACDQCGHQRIAYNSCRNRHCPKCQATAAAQWMEAREAELLPVEYFHVIFTL
                     PTALGPIALQNPREVYGLLFRAAAETLQQIAADPKHLGAEIGFLAVLHTWGQNLQHHP
                     HLHCVVPGGGLSLDGSRWVACRPGFFLPVRILSRVFRGKFLALLGEAFDHGKLSFHGK
                     LSARADAGEFHRRVTASARTEWVVYAKPPFGGPEQVLKYLARYTHRVAISNRRLVALE
                     DGEVT"
     gene            complement(842..1702)
                     /locus_tag="AC16G0_12030"
     CDS             complement(842..1702)
                     /locus_tag="AC16G0_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR5100377.1"
                     /translation="MTPLRQRMIEDMKLRNFAPRTIQVYVERVATFAKHFGQSPDRLG
                     PADVRAYLLYLVNQKHASWSYYNQALCALRFLYRVTLGKKWVLDGVVCPKQPKRLPVV
                     LSPAEVTHFFEAIPGLKHRALLMTAYAAGLRVSEVVALRVDDIDSQRMVIRVRQAKGR
                     KDRYVMLSPRLLEILRQYWKAARPDRYLFPGQDPEKTLHTRTVQQVCRDACQAAGLGK
                     HVTVHTLRHSFATHLLEAGTNIRTIQILLGHRSLKTTAVYTHVSAVALEATQSPLDRL
                     APPMEGRSQP"
     gene            2055..>2183
                     /locus_tag="AC16G0_12035"
     CDS             2055..>2183
                     /locus_tag="AC16G0_12035"
                     /inference="COORDINATES: protein motif:HMM:NF023168.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HIR5100378.1"
                     /translation="MARPPKLAPEQIRQTIQELAAEGAELSITAIRERLGTGSYSTI"
BASE COUNT          406 a          646 c          760 g          371 t
ORIGIN      
        1 aacgtgactt cgccatcttc cagggcgacg agccgtcggt tgctgatcgc aacccggtgg
       61 gtgtaccgcg ccaggtactt cagtacttgt tccggtcctc cgaaaggggg cttggcgtac
      121 acgacccact cggtccgggc gctggcggtg acccgtcggt ggaactcgcc ggcatccgcc
      181 cgcgcgctga gcttcccgtg gaacgagagc tttccgtgat cgaaggcctc gcccagcagc
      241 gcgaggaact tgccccggaa gacacggctg aggatgcgca ccgggagaaa gaatcccggc
      301 cggcaagcca cccaacgtga gccgtcgagg gacaacccgc ctcccggcac gacgcaatgc
      361 agatgcgggt ggtgctggag attctggccc caggtatgca agacggccag gaatccgatc
      421 tcagccccga ggtgcttcgg atcggcggcg atctgctgca aggtctcggc ggcagcacgg
      481 aacaggaggc cgtagacctc ccgcgggttc tgtagggcga tcggacccag tgccgtcgga
      541 agcgtaaaga tcacatggaa gtattcgacg ggcagcagct ccgcctctcg ggcctccatc
      601 cactgcgccg cggcggtggc ctggcacttg gggcagtggc ggttgcggca cgagttatag
      661 gcgatccgct gatgcccgca ctggtcgcat gcctcgacgt gtccgcccag cgcggcggtc
      721 cggcaggccg cgatgtcgcg cagggcgcgg cgacgctcgg gcgagagcgt gtcgccgtac
      781 tgttcgagga aggcatgccc atgttcgcgg aagacatcag ccacttcgag ccgatgttgc
      841 gtcatggctg ggatcgtccc tccatcgggg gtgcgagccg atcgagcgga ctttgcgtcg
      901 cctccagggc caccgccgag acgtgggtgt agaccgccgt ggtcttgagg ctgcgatgcc
      961 cgagcaggat ctggatggtc cggatgttgg tcccggcctc caggaggtgg gtggcgaagc
     1021 tgtgccgcag ggtatgaacg gtgacgtgct tgcccaatcc cgcggcctga caagcgtccc
     1081 ggcagacctg ttgcaccgtg cgggtatgga gcgtcttctc cggatcttgc cccgggaaga
     1141 ggtatcggtc cggacgggcc gccttccaat actggcgaag gatctcgagc aaccgggggg
     1201 agagcatcac atagcggtcc ttgcggccct tggcctggcg gacgcggatg accatccgct
     1261 ggctgtcgat gtcgtcgaca cgcaaggcca cgacctccga gacgcgcaac ccggcggcgt
     1321 aggcggtcat caggagggcg cggtgcttga ggccggggat ggcctcgaag aagtgcgtga
     1381 cctcggcagg gctcaagacg acggggagcc tctttggctg cttgggacag acgacgccgt
     1441 cgaggaccca cttcttgccg agggtgacgc gatagaggaa ccgcagggcg cagagggcct
     1501 ggttgtaata gctccacgag gcgtgcttct ggttgacgag gtagaggaga taggcacgga
     1561 cgtcggccgg cccgaggcgg tcgggcgact ggccgaagtg cttggcgaag gtggcgaccc
     1621 gttcgacgta gacctggatg gtgcggggtg cgaagttgcg gagtttcatg tcctcgatca
     1681 ttcgctggcg cagcggtgtc atgacgggct cctgtgctga ggtggaaggg tgggggggaa
     1741 ccctcatcat ggctcagcag gagcgtggga tgggcaccga ggttagggag gaccgaggag
     1801 atcgagaggg agtcggtcag gaagggcttg gaagaaggga cagccttcga ccagcgagaa
     1861 gactggcgct atgggactct accgcgaagc ggtttagtac aacgcccttt atgcgagcca
     1921 tccgagcccc ccgcaggagc cccaaaaccc acaccttaca gcccccacat cgacgttgac
     1981 ccgcccggcg gttctgtaat atcatattat gtaataatgt aattatataa ttatataact
     2041 agtgcatgag gtacatggcc cgaccaccaa aactcgcccc agagcaaatt cgccagacca
     2101 tccaagagct cgccgctgag ggggcagagc tctcgatcac tgccatccgc gagcggcttg
     2161 gcaccggcag ctactcgacc atc
//
LOCUS       DBNGZC010000697         3671 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1131596, whole genome shotgun sequence.
ACCESSION   DBNGZC010000697 DBNGZC010000000
VERSION     DBNGZC010000697.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1131596"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1162)
                     /locus_tag="AC16G0_12040"
     CDS             complement(104..1162)
                     /locus_tag="AC16G0_12040"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR5100379.1"
                     /translation="MNVKERILAAMTWGEPDRIPLTIYDWMLPRGSTERLLRESGVGL
                     ITRLPAHQVEQREVEIITREYQEKGRRLIRRTIHTPVGDVWQTLEPDAAYGTSNWIQE
                     HFIKGPEDYRVMEFYYLDGIYRDNYSLIRESQRRMGDDGIVLVRIAKSPIQEMLYQMM
                     GLERFAIDFHERRDLFDSLHATMLKRYEELYDLAAGSPVEILQLGDNISSDVVGKERF
                     RSYLMPEYRKIMARLAGTGKKLAVHMDGNLESLQGEIGEAQFDIVEAMTPPPMGDVSV
                     KAARERWPGKALWINFTSSMHIEPPEVIEEHTRSLVEEAGTKRGFAISVTEDAPVEAL
                     ERSLAVISRVLREPGSGA"
     gene            complement(1412..1858)
                     /locus_tag="AC16G0_12045"
     CDS             complement(1412..1858)
                     /locus_tag="AC16G0_12045"
                     /inference="COORDINATES: protein motif:HMM:NF024189.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HIR5100380.1"
                     /translation="MSRQLQPSRARREQTHRAAASAWGPLGPHGLSTPCWRRRCAVIT
                     RVARIISAGLLLAAAGCVATLPEQAVVQTPPPFPPPQVEVAPVQPGPAYVWVPGHWAW
                     RGPRRGYVWIGGYWAVPQAPTYVWVPGHWEPRRGGHVWVEGYWRPR"
     gene            2216..2827
                     /locus_tag="AC16G0_12050"
     CDS             2216..2827
                     /locus_tag="AC16G0_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017748838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR5100381.1"
                     /translation="MSQRPSKDPPDQHTFSRRAFLKTVGVGTAVTGLVGAVPRPARAE
                     ILGPDAVPLSLKVNGAVHSLTVEPRVTLLRALRNHLGLTGAKEVCERGACGACTVLLD
                     GEPVCSCMLLAVDAAGHEITTVEGLGTPEKMSPVQAAFVEADALQCGFCTPGFVVTST
                     ALLRKNPNPTLEEIKAGLAGNLCRCGTYSRIFEAVQKAAKQRG"
     gene            2831..>3671
                     /locus_tag="AC16G0_12055"
     CDS             2831..>3671
                     /locus_tag="AC16G0_12055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR5100382.1"
                     /translation="MAQQDPAGDSTFQVKVGPAEHPVTLEVHPAAGDLKPWDNDTRLR
                     AVKGRHPRQEAPLKVTGRAEYTYDVRLPGMLWAKMVRAPIPGGKIVRIDTAKAERLPG
                     VQAIWTTDAKLVRFVGQDIAAVAAVSQEIAEDGARLVEVEYQAAPFVTDLEKAMEPTA
                     PLVYAGGQAPASPNAPRKGNVTGPLPAAAVGGARGGTRGDIEKGFAEADTVHEATYRL
                     AVHTHAVLETHGAVAHWEGDRLTVYTSTQGVFMVRAALAETFGLPRNKVRVISEHMGG
                     GFGS"
BASE COUNT          642 a         1205 c         1194 g          630 t
ORIGIN      
        1 gccggcggtc tttcttccat gccccctcgc tccaggaacg gatcccagcc tcgcttggcc
       61 tcccaggcat ccgggggcac aggaggccgc agaccgacgc gcctcaggct cccgacccgg
      121 gctcccggag gacgcgcgag atgacggcga ggctccgctc cagggcctcg accggcgcgt
      181 cttcggtcac gctgatggcg aagccccgtt tggtcccggc ctcctccacg agcgacctgg
      241 tgtgttcctc gatgacctcg ggcggttcga tatgcatcga gctggtgaag ttgatccaca
      301 gcgccttgcc cggccagcgt tcccgtgccg ccttcaccga gacgtcgccc atgggagggg
      361 gcgtcatcgc ctcgacgata tcgaactggg cctccccgat ctccccctgc agggactcca
      421 gattgccatc catgtgcacg gccagcttct tccccgtccc cgcgaggcgc gccatgatct
      481 tcctgtactc cggcatgagg taggagcgga accgctcctt ccccaccacg tcgctgctga
      541 tgttgtcgcc gagttggagg atctccaccg gtgagccggc ggcgaggtcg tacagctctt
      601 cgtaccgctt gagcatggtg gcatgcaggg aatcgaagag gtcgcgccgc tcgtggaagt
      661 caatggcgaa ccgctccaat cccatcatct gatacagcat ctcctggatg ggagacttgg
      721 caatgcgcac gaggacgatg ccgtcgtcac ccattcgccg ctgactctcc cggatcaggc
      781 tgtaattgtc ccggtaaatg ccgtccaggt agtagaactc catcacccgg tagtcttcgg
      841 gtcccttgat gaagtgctcc tggatccagt tcgaggttcc gtacgcagcg tccggctcca
      901 aggtctgcca cacgtcaccc acgggcgtgt ggatggtccg acgaatgagc ctgcggccct
      961 tctcctgata ctcccgcgtg atgatctcga cctcgcgctg ctcgacctgg tgggccggga
     1021 ggcgggtgat caacccgacg cccgattcgc gcagcaaccg ctcggtcgac cctcggggga
     1081 gcatccaatc gtaaatggtg agtggaatcc gatccggctc accccaggtc atcgcggcca
     1141 ggatacgctc cttgacgttc acgcagcctc ctcccggttt cggcagccaa caacccctaa
     1201 cccggattat tcgcgaacga gttcgcgaat agtcgcggga atgaccaaat cccaatgccc
     1261 aatgaccaat tgcggattgc cccttcgtga tcctccgtga cggcgcgctg tacgcgccca
     1321 ccatcattgg tcattggaaa ttggtcattg gtcatttgat ccggagaatt cacgagtcca
     1381 ttcgtgaata atccggacta agagatgggt ctcagcgcgg tcgccagtag ccttccaccc
     1441 acacgtgccc gccgcggcgc ggctcccagt ggccaggaac ccagacgtag gtgggtgcct
     1501 ggggaacggc ccagtagccg ccaatccaga cgtatccgcg gcgcggacca cgccatgccc
     1561 aatggcccgg aacccagaca tacgcggggc ctggctggac gggagcgacc tcgacttgcg
     1621 ggggcggaaa tggcggcgga gtctgcacga ccgcctgctc gggaagggtg gccacacacc
     1681 cggcagcggc gaggaggagt ccggcactga ttattcgggc tacccgcgta atcactgcac
     1741 atctcctgcg ccaacagggt gttgacagcc catgcggccc aagaggcccc caggcagatg
     1801 cggccgccct gtgtgtttgc tctcttcggg cacgagatgg ttgaagctgc cgcgacattt
     1861 cctcacgtga attgtaaccc gccgaagtct atgggggctg gccgcggctg tcaaggagct
     1921 ggacggaggg gggttcagga tcggcaaagc aggagcgacc atggatattc ggcccgcggc
     1981 gctgggctca aaacagcgaa gagaacacgg gcaatgtgca acaggagaaa ccgaacattc
     2041 catatgacct ctgccgtcga cctccgcggg cggtgagttt gtattcaaaa tgggacatcc
     2101 aattgacaca agaaatgatc cgggtagtat ctcgtcttgc tactgggtcc ctgcttctcg
     2161 cgctcccgcc tccgaggtcg ggtcacagaa ttgctgtcct cttgcgaggt gtcttatgag
     2221 ccagcgtccc tccaaagatc ctcccgatca gcacacattt tcgcggcgcg cgtttctcaa
     2281 gaccgtgggc gtcggaacgg cggtcacggg actcgtcggc gcggtccctc gcccggcccg
     2341 ggcggagatc ctgggacctg atgcggttcc gctgagcctc aaggtgaacg gcgcggtcca
     2401 cagcctgacc gtggagccgc gggtcacact gctgcgggcc ctgaggaatc acctgggcct
     2461 cacgggcgcc aaggaggtct gtgagcgcgg cgcttgcggg gcgtgcacgg tccttctgga
     2521 cggcgagccg gtctgctcct gcatgctgct ggcggtggat gccgccgggc acgagatcac
     2581 gacggttgag ggtctcggga caccagagaa gatgagcccg gtgcaggccg ccttcgtgga
     2641 ggccgacgcc ttgcagtgcg gcttctgcac gccgggcttc gtggtgactt ccaccgccct
     2701 cctgcgaaaa aaccccaacc ctaccctgga agagatcaag gccggcctgg cagggaacct
     2761 gtgccggtgt gggacgtact cccgcatttt cgaggccgtc cagaaggcgg ccaagcagag
     2821 ggggtgaccc atggcgcagc aggaccctgc cggtgacagc acgttccagg tcaaggtcgg
     2881 acccgccgaa cacccggtga ccctggaggt ccatcccgcg gcgggcgacc taaagccctg
     2941 ggacaacgat acccgtcttc gggccgtcaa gggacggcac ccccggcagg aagcgccgct
     3001 gaaggtgacg ggccgggccg agtacaccta tgacgtgcgg ctcccgggga tgctctgggc
     3061 caagatggtc cgggcgccaa ttcctggcgg gaagatcgtc cgaatcgaca cggcgaaggc
     3121 cgagcggctt ccgggcgtgc aggcgatttg gaccaccgac gcaaagctgg tccgcttcgt
     3181 cggccaggac atcgcggcgg tcgctgccgt gtcccaagag atcgcggagg atggggcgcg
     3241 gctggtggag gtcgagtacc aggcggcgcc cttcgtcaca gatctcgaaa aggccatgga
     3301 gcctacggcc cccctggtgt acgctggggg gcaggccccc gcctccccga acgccccgcg
     3361 gaaggggaat gtgaccggac cgctcccggc tgcggcggtc ggcggcgcgc gcggggggac
     3421 ccggggggac atcgagaagg gcttcgccga ggccgatacg gtccacgagg ccacgtaccg
     3481 tcttgccgtc cacacccatg ccgtcctcga gacgcacggg gccgtcgccc actgggaggg
     3541 ggaccggctc acggtctaca cctccacgca gggggtcttc atggttcgcg ccgccctggc
     3601 cgagacgttc ggtctcccgc gcaacaaggt ccgcgtgatc tcggagcaca tgggcggcgg
     3661 cttcgggagc a
//
LOCUS       DBNGZC010000698         1791 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1722051, whole genome shotgun sequence.
ACCESSION   DBNGZC010000698 DBNGZC010000000
VERSION     DBNGZC010000698.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1722051"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="AC16G0_12060"
     CDS             <1..969
                     /locus_tag="AC16G0_12060"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR5100383.1"
                     /translation="KDARGRAILRALAKRSDVLLENFKPGTLARLGLDYADLIQDNPR
                     LIFCAISGFGRTGPYAERGGFDVIVQAVGGLMGITGDPDGPPVKVGVAMTDICTALHA
                     HGAILAALYARERTGKGQRIDVSLLETQIAALINIASSYLNAGELPRKWGTAHASIVP
                     YQAFRAQDGYMIIGGATDRLWLKLCEAIGLPELARNPNYLTNEQRVQHREEIVRLLEE
                     RLMTRTRTEWETILAPTGVPCGPINRMDEVFADAQVRHLEMVLEGTHPQVGPIRMVRN
                     PVTFSETPAELRLVPPKLGEHTEEVLRDLLGYSADEIADLRAAGVV"
     gene            1064..>1791
                     /locus_tag="AC16G0_12065"
     CDS             1064..>1791
                     /locus_tag="AC16G0_12065"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5100384.1"
                     /translation="MIDILIRGVQVFDGGGDPPVESDIAIAGDRIQAVGHKLSAQAAH
                     RVIEARGLAACPGFVDIHGHSDYHLLLTPTAESALLQGVTSEIGGNCGHSAAPIWGPW
                     LEDRARSYREVYGLDHDWREVGAYFRRLLDPGISINFGLLAGHNTLRGSAMGGADRAP
                     TAEELQAMISGLERGMEEGALGLSTGLVYSPACFSSPDELATLTAAAGRMGGILTTHM
                     RSEGDDLLESIQEVISAAETARTPL"
BASE COUNT          314 a          558 c          608 g          311 t
ORIGIN      
        1 aaggatgctc gcgggcgggc catcctccgg gccctggcca agcgctcgga tgtcctcttg
       61 gagaatttca agccgggcac gctggccagg ctggggctgg actacgcaga cctgatccaa
      121 gacaaccccc gcttgatctt ttgcgccatc tcggggtttg gccggaccgg cccctacgcg
      181 gagcggggcg gtttcgacgt gatcgtgcag gcggtgggcg gtctcatggg catcaccggc
      241 gacccagacg gcccaccggt caaggtgggc gtggccatga ccgatatctg cacggccctc
      301 cacgctcacg gcgcgatcct ggccgcgctc tacgcccggg aacgaacagg aaagggacag
      361 cggatcgacg tctcgctcct ggagacgcaa atcgcggcgc tcatcaacat cgccagctcc
      421 tacctgaacg ccggcgagct tccgcggaaa tgggggacgg cccacgccag catcgtcccg
      481 tatcaggcct tcagagcgca agacggctac atgataatcg gaggcgccac ggatcgtctg
      541 tggctgaagc tgtgcgaggc catcgggctc ccggagttgg cgcggaaccc caattacctt
      601 accaacgagc agcgcgtgca gcaccgggag gagatcgtcc gcctcctgga agagcgactg
      661 atgaccagga cccgaacgga gtgggagacc attctggcgc ccacgggcgt tccctgcgga
      721 cccatcaatc ggatggacga ggtctttgcc gacgcccagg ttcggcacct ggagatggtg
      781 ctggagggga cccatcctca ggtgggtccc atccggatgg tgcgaaaccc ggtgacattc
      841 tccgagacgc ctgcggagct ccgactggtt ccgccgaagt tgggggaaca caccgaggag
      901 gtccttcgag acctcctggg gtactccgcg gacgagatcg cagacctccg ggctgcaggc
      961 gttgtgtgag aggtgaaatg accgatgatc aatcgccaat gaccgatggg ctctcagatc
     1021 tcatagggac ttggtcattt cctcgaagtg gcggggcatc ggtatgatag acattctgat
     1081 ccgcggggtg caggtgttcg atggcggggg cgatcctcct gtcgagtccg atattgccat
     1141 cgcaggagat cgcatccagg ctgtgggaca caagctctcg gcgcaggctg cacaccgggt
     1201 catcgaggcc cgtggacttg cggcctgtcc aggctttgtg gacatccacg ggcactccga
     1261 ttatcacctg ctgctcacgc ccacggccga gagcgccctg ctgcaggggg tgacctccga
     1321 gattggcggt aattgcggcc attccgcggc accgatctgg ggcccctggt tggaggaccg
     1381 cgcccggtcg tatcgcgagg tctatgggct agaccacgac tggcgcgaag tgggggcgta
     1441 tttccgccgg ctccttgacc cggggatctc gatcaacttc ggcctcctgg ccggtcacaa
     1501 caccctccgg gggtccgcca tggggggtgc ggatcgcgcg cccacagcgg aagagctgca
     1561 ggctatgatc tccggcctgg agcggggcat ggaagagggg gcgctgggcc tgtccaccgg
     1621 cttggtctac tcgcctgcct gcttttcctc gccggacgag ctggcaaccc ttaccgccgc
     1681 tgcggggagg atgggcggga ttctcacaac gcacatgcgg agcgaggggg atgacctgct
     1741 ggagtcgatt caagaggtca tttccgcggc cgagacggca cggacgcccc t
//
LOCUS       DBNGZC010000699         1482 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2015379, whole genome shotgun sequence.
ACCESSION   DBNGZC010000699 DBNGZC010000000
VERSION     DBNGZC010000699.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2015379"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..1397)
                     /locus_tag="AC16G0_12070"
     CDS             complement(255..1397)
                     /locus_tag="AC16G0_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR5100385.1"
                     /translation="MAILAGGMGLCLIVVIMWETFEVIVLPRRVTRRVRLTRLFYRAT
                     WVMWSTIVGRLRSSKRQETYLSFYGPLSLLLLLSVWAAGMILGFALFQAALGSALNVS
                     AGTASFGTDLYMSGTNFFTLGLGDVTPRTTLARLFTVVEAGTGFGFLALVIGYLPVMY
                     QAFSRREVSTVLLDARAGSPPCAAELLRRCGQSLRQELDPLLREWERWSAELLESHIS
                     YPVLCYYRSQHSNQSWLAALTAILDTSALVLVGVDGISPRQAQLTFAIARHAIADLAQ
                     IFKTPPRMPGADRLPPPSLIRLREGLAAAGVRLRDGVTADRRLDELRRMYEPYANALA
                     EYLLMSLPPWLPAAGGFDSWQTSAWERISPGEVTSAAVATQRDEHF"
BASE COUNT          293 a          503 c          452 g          234 t
ORIGIN      
        1 ggccggctgc cgagaccatc acgatcgggg caactgcctg ggtgatcact tggatgtccg
       61 ctgattccat cacgaaggcc tccgttgcat cccgcccacc ggtcaggcca tctcacgctt
      121 gaactgggag gagaccgggc aattcgagga aacggcaggc cggttcactc tggtctccta
      181 gcgtcccgct caatcgcaag ggtcccaagc acctccggat ccgagccgat gtcctaccgc
      241 cgaaccgcgg gcccctaaaa atgctcgtcc cgctgcgtcg ccacagccgc gctcgtcacc
      301 tctccgggcg aaatccgctc ccaggcactc gtctgccagc tatcgaagcc tccagccgct
      361 ggcagccacg gcggaaggga catcagcaga tactcggcca gggcgttggc gtacggctcg
      421 tacattcgcc gcaattcatc cagtcgtcga tcggcggtga caccatcgcg cagtctcacg
      481 ccggccgcgg caaggccttc tcgcaagcgt atgagactgg gtggagggag gcggtccgct
      541 ccgggcatcc ggggtggcgt cttgaagatc tgcgccaggt ccgcgatggc gtggcgcgcg
      601 attgcgaatg tgagctgcgc ctgccgcggc gaaatcccgt cgaccccgac caagaccagc
      661 gcactggtgt ccaggatggc cgtcagcgcg gccagccagg actggttgct gtgttgcgag
      721 cggtaataac acaggaccgg ataggagatg tggctttcca gcagttctgc cgaccagcgt
      781 tcccactcgc ggagcaaggg gtcgagttcc tgacgcaggc tctgcccgca gcggcggagc
      841 aattctgccg cgcagggcgg cgagcccgcg cgggcatcca gcaggacagt actcacttcc
      901 cgccgggaga aagcttgata catcacgggg agatagccga tgaccaatgc gagaaagcca
      961 aagcccgtcc ctgcctccac aaccgtgaag agccgcgcca aggtcgtgcg gggggtcaca
     1021 tcgccgaggc ccagggtaaa gaagttggtg ccgctcatgt acagatccgt cccaaaactg
     1081 gccgtccccg ccgacacgtt cagcgccgac ccgagcgccg cctggaacag ggcgaacccc
     1141 agaatcatac ccgccgccca cacgctcaac agtagcagga gggacaaggg tccgtagaag
     1201 ctcaggtatg tttcctgccg cttgctggat cgaaggcgtc ccacgatggt cgaccacatc
     1261 acccaggtgg cgcgatagaa aaggcgcgtc aggcgaaccc ttcgtgtcac acgtcggggc
     1321 aggacgatga cctcgaaagt ttcccacatg atgacaacga tcagacagag ccccatcccg
     1381 ccggcaagga tcgccatcgc ccccctcccc aatccccgag tctccaaccc gcgtcggttt
     1441 gaccatgcgg ccggccgtgg ccgaccttgt ttcagccgac at
//
LOCUS       DBNGZC010000700         1698 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_159026, whole genome shotgun sequence.
ACCESSION   DBNGZC010000700 DBNGZC010000000
VERSION     DBNGZC010000700.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_159026"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..1541
                     /gene="ltrA"
                     /locus_tag="AC16G0_12075"
                     /pseudo
     CDS             266..1541
                     /gene="ltrA"
                     /locus_tag="AC16G0_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020393465.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          307 a          507 c          587 g          297 t
ORIGIN      
        1 tggcgtgagc tcgtcgcggg cgctggcatg gaacaggaga acctgtcgcc tcgataccga
       61 cgaccgggtg atatcggcgc gggttgccga ttccggtcgc aagagggagg accccaagtg
      121 gtcgaaaccg caaggggcag agtaccgatg cgaggcacag gggcgggccg gctcgtagta
      181 gcggtgaagg ctctgtaatg gggctggagc gaaggggccg ggcaggccag atcgacctga
      241 ggtcaccccg taagggtagg agctgatggt tggatcgaag tcgaaggtga agtcgtttga
      301 gatcgataag cgcctcgttt atgaggcgtg gatgaaggtc caggccaacg gcggagcgtc
      361 gggcgtggac gccgtcagca tcggcgggtt ccgtgaggca cagagggaca acctctacaa
      421 gctgtggaac cggatgagct ctggcagcta cttgccgggc ccggtgcggg cggtggagat
      481 accgaaggat catggggcgg ggatcagggt gttgggtgtg cccaatgtgg ccgaccgaat
      541 cgcccagacc gcggctgcga tgctgctgga ggagaagctg gagccgatct tccacccgga
      601 cagctacggt taccgtcctg ggcgttcggc ccacgacgcg ttagccgtaa ctcgacggcg
      661 ctgctggaaa cagggctggg cgttagatct tgatatccgg gcctttttcg acagcgtccc
      721 gcacgatctg ctactcaagg cggtcgctca ccacaccaac gagcgttggg ttctgctcta
      781 catcaagcgg tggcttaccg cccccatgca gatgccggac gggactctga gagcgcggga
      841 caaggggacc ccgcaggggt cgccgatctc tccgttgttg gccaacgtgt tcatgcatta
      901 cgcgttcgat gtctggatgg ctcggaagtt cccgggctgc cccttcgagc ggtttgcgga
      961 tgacattgtc gctcactgcg actccgagga tcaggcccgc gcgctgcggg actcgatcgc
     1021 cacccggctc ggggcacttg gcctcgagct gcatcccgag aagaccaagg tcgtgttctg
     1081 ccaggacgcg aaccggccgg ggaaagccga gcacaccagc ttcgacttcc ctcggctaca
     1141 ccgtccgggg gcgcctggcc aaaggccgca gaggctactt cgtgagcttc gccccggcca
     1201 tcagtgtcaa ggcgcggaag gcggtcggcc agaaggtcag ggcctggcac ctcaatcgtc
     1261 gcagtgggac agatctgtcc ggcctcgccg aagagatcga ccctcaggtg cgcggctgga
     1321 tcgactatta cggggccttc taccgctccg agttgtattc cctcgcatgg cgcatcgacg
     1381 agcatctcgt tcgatgggcg ttgcagaaat acacacgact gcgcgggaaa gccgccaagg
     1441 cacgggcctg gctggacgcg gtgcgacagc accagccccg actctttgcc cactggtacc
     1501 ttctcccgcc cacccgaggc gggcctgtgg gagccgtatg acgggagact gtcacgtacg
     1561 gttctgcgag agcccggggg tgcgattccc ccgggccact ctcctcgtca tgggattcca
     1621 gtaccgaaac gacgccgagc gatgtctccg ggaactccgg gaccgcctgg tcacgttcgg
     1681 gttggagcta catcccga
//
LOCUS       DBNGZC010000701         1629 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1430804, whole genome shotgun sequence.
ACCESSION   DBNGZC010000701 DBNGZC010000000
VERSION     DBNGZC010000701.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1430804"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..1076)
                     /gene="hpnD"
                     /locus_tag="AC16G0_12080"
     CDS             complement(216..1076)
                     /gene="hpnD"
                     /locus_tag="AC16G0_12080"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046905 - 15-cis-phytoene synthase
                     activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HIR5100386.1"
                     /translation="MCQSLTKASGSNFAYAFRFLPKPQRKALYAIYAFCRVTDDLVDE
                     GASLAQDAGPEGNGPALERLKAWRAELDACFRGEASHPVTQRLAETIQAFRIPHAYFQ
                     ELLTGVEMDLVQCRYATFAELQQYCYHVAGVVGLMCIEVFGYSQPECRTYAERLGTAF
                     QLTNILRDLGGDAARGRIYLPQEDLQRHGYSEADLLDRKVTPAFRALMQFEVARAREF
                     YAAAQAALPAQDRRAMLPAEIMTAIYRRILERIETTDYDVFSRRIRLSDPYRLLLALG
                     CWARRRLLPC"
     gene            complement(1178..>1629)
                     /gene="ispH"
                     /locus_tag="AC16G0_12085"
                     /gene_synonym="lytB"
     CDS             complement(1178..>1629)
                     /gene="ispH"
                     /locus_tag="AC16G0_12085"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF014456.7"
                     /note="catalyzes the conversion of
                     1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into
                     isopentenyl diphosphate (IPP) and dimethylallyl
                     diphosphate (DMAPP); functions in the nonmevalonate
                     isoprenoid biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR5100387.1"
                     /translation="DTSAIIGALRRRFPTITAPARDDICYATQNRQMAVKALASRVPV
                     ILVIGSDNSSNSNRLAEVARDGGARAYLIDDISQIDPAWLEGVECLGITAGASAPEYL
                     VDEVVQYFRKGGVTEIEEVQAVTEEVSFALPPELVRDRAARESAQAA"
BASE COUNT          274 a          509 c          575 g          271 t
ORIGIN      
        1 tcgaagcgtg gagtacatcc cgggggtcag catgaccgac ctgcaccagg aggattcggc
       61 tggaaccgag ccggggccca ctgataaagg gctcttggga tgaacgtccc gaagagtttc
      121 gatgttgttg aacttctgga ttgatgaggg ctggcgggtc ttcgcggtct tggcaatctt
      181 ggcggtgaaa tttcgggatt aggaagggga ggcgatcagc aggggaggag gcggcgcctg
      241 gcccagcacc ccagggccag cagaaggcga taggggtcgg agaggcgaat gcgccgggag
      301 aacacatcgt agtccgtggt ctcgatgcgc tccagtatcc tgcgatagat ggccgtcatg
      361 atctcggcag gcagcatggc ccggcggtcc tgggcgggca aggccgcctg ggcggccgcg
      421 tagaattccc gggcgcgggc cacctcgaat tgcatcaggg cgcgaaaggc cggcgtcacc
      481 ttccggtcga ggaggtccgc ctcgctgtag ccgtggcgct ggagatcctc ctggggcaga
      541 tagattcgcc cccgcgcggc gtcgcctccc aggtcccgca ggatatttgt gagctgaaag
      601 gcggtgccca ggcgctcggc gtaggtccgg cactcgggct gggaatagcc gaagacctcg
      661 atgcacatca ggccgacgac acccgccacg tggtaacagt actgctgtaa ctccgcgaag
      721 gtcgcgtagc ggcactgcac caggtccatc tccacgccgg tcaggagctc ctggaagtac
      781 gcgtggggga tccggaaggc ttggatggtc tccgccagtc tctgcgtgac ggggtgcgag
      841 gcctcgcccc ggaagcaggc gtccagctcg gcacgccagg ccttgagccg ctccagggct
      901 ggcccattcc cttccggccc ggcatcttgg gcgagcgacg cgccctcatc caccagatcg
      961 tcggtcacac ggcagaaggc gtagatggcg tagagcgcct tgcgctgggg cttggggagg
     1021 aaccggaagg cgtaggcgaa gttggacccg ctggccttgg tcagggactg gcacacgtcc
     1081 cggacaccta cggcaggaaa gccagtacgg atcatcctgt gtcacattcc ccgacgtgcg
     1141 tggagccggt tagggactgc tggcgccggt gaacccgtca cgcggcctgc gccgattccc
     1201 gggccgcgcg atcccgtacg agttcagggg gcagggcgaa gctgacctcc tcggtcaccg
     1261 cctggacctc ctcgatctcg gtcacgccgc ccttccggaa gtactgcacc acctcgtcca
     1321 ccaggtattc gggcgccgag gcgccggcgg tgatcccgag gcactcgacc ccctccagcc
     1381 aggcgggatc gatctgggag atgtcgtcga tgaggtaggc ccgggcgccc ccatcccggg
     1441 cgacctccgc cagccggttg ctgttggagc tgttgtccga cccgatcacc aggatgaccg
     1501 ggactcggct ggcaagggcc ttcaccgcca tctgacggtt ctgcgttgca tagcaaatat
     1561 cgtcccgggc cggcgccgtg atcgtgggga agcgccgccg gagcgcgccg atgatggccg
     1621 aggtgtcgt
//
LOCUS       DBNGZC010000702         3839 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1036352, whole genome shotgun sequence.
ACCESSION   DBNGZC010000702 DBNGZC010000000
VERSION     DBNGZC010000702.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1036352"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..635)
                     /gene="rplF"
                     /locus_tag="AC16G0_12090"
     CDS             complement(81..635)
                     /gene="rplF"
                     /locus_tag="AC16G0_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR5100388.1"
                     /translation="MSRIGRLPIPIPDAVKVDVKDHTVSVQGPKGSLSFTAHPSMRVE
                     VKDRQVVCGRPTDEKFHKALHGLTRSLIANMVAGVVKGFERKLELVGVGYRASTMGPN
                     LNLSLGYSHPIIYPVPSGIRIEVKDQTQITVSGIDKQLVGAVAAKIRSFRPPEPYKGK
                     GVKYEGEYIRRKAGKAAAASGAKA"
     gene            complement(670..1068)
                     /gene="rpsH"
                     /locus_tag="AC16G0_12095"
     CDS             complement(670..1068)
                     /gene="rpsH"
                     /locus_tag="AC16G0_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003241998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR5100389.1"
                     /translation="MSMTDPIADMLTRIRNANQAQLERVDIPASRLKVELAKVLKAEG
                     FIRGYKLADDSRQGILRVYLKFGPGNERVIQGLKRVSRPGLRVYRKAAQIPRVFSGMG
                     VAILSTSQGLMTGKAARERSVGGEVLCYVW"
     gene            complement(1147..1332)
                     /locus_tag="AC16G0_12100"
     CDS             complement(1147..1332)
                     /locus_tag="AC16G0_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIR5100390.1"
                     /translation="MAKTSLIIKQQRPPRFAVRRYHRCRICGRPRGYMGKFQMCRICF
                     RGLALRGEIPGVLKASW"
     gene            complement(1345..2016)
                     /gene="rplE"
                     /locus_tag="AC16G0_12105"
     CDS             complement(1345..2016)
                     /gene="rplE"
                     /locus_tag="AC16G0_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR5100391.1"
                     /translation="MADKAERKARADAGKAAKGEKGAKGERGAKGKPEAAAAPQVPRV
                     PARLRARFRGEITQALMTEFGYANRMQVPEVKKVVINMGLGEAVGNVKVIDAAVEELA
                     AITGQRPVVTRARKSEAAFKLRAGMPIGCKVTLRGERMYEFLDRLLSVALPRIRDFRG
                     VPAKSFDGRGNYALGIREQLIFPEIRYDKVDMTRGMDVCIETSARNDEQARALLTHLG
                     FPFRK"
     gene            complement(2020..2349)
                     /gene="rplX"
                     /locus_tag="AC16G0_12110"
                     /pseudo
     CDS             complement(2020..2349)
                     /gene="rplX"
                     /locus_tag="AC16G0_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743141.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
     gene            complement(2389..2757)
                     /gene="rplN"
                     /locus_tag="AC16G0_12115"
     CDS             complement(2389..2757)
                     /gene="rplN"
                     /locus_tag="AC16G0_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR5100392.1"
                     /translation="MIGLRTILDVADNSGAKRISLIKVLGGSAKRYAHIGDIIVANVK
                     EAVPEGTVKKGTVVKAVVVRTVKEQRRPDGSYIKFDRNAAVLIDDHNDPVGTRIFGPV
                     ARELREARFMKIISLAPEVI"
     gene            complement(2812..3084)
                     /gene="rpsQ"
                     /locus_tag="AC16G0_12120"
     CDS             complement(2812..3084)
                     /gene="rpsQ"
                     /locus_tag="AC16G0_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR5100393.1"
                     /translation="MAPNMTEDRSRRQTVVGMVVSDKMQKTVVVEIARLVRHQSYDKM
                     VRRKTKVKAHDEQNACHVGDRVELMETRPLSKDKRWRVVEILQRAV"
     gene            complement(3086..3298)
                     /gene="rpmC"
                     /locus_tag="AC16G0_12125"
     CDS             complement(3086..3298)
                     /gene="rpmC"
                     /locus_tag="AC16G0_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR5100394.1"
                     /translation="MKYAELKDLTAEELRQKEAALREDLFKLRLRQASTQVENPMRIR
                     QLRHDIARVQTAQRALAATTASKEGV"
     gene            complement(3298..3720)
                     /gene="rplP"
                     /locus_tag="AC16G0_12130"
     CDS             complement(3298..3720)
                     /gene="rplP"
                     /locus_tag="AC16G0_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR5100395.1"
                     /translation="MLVPKRVKFRKQQRGRRSGIATRGSVLAFGQYGLKAMEAAWITN
                     RQIEAARRAMTRYVKRGGKVWIRIFPDKPITAKPAETRMGKGKGAPEGWVAVVKPGRI
                     LYEMEGIPEAEAKEAMRLAAMKLSIATRFVTRGVGGTM"
BASE COUNT          549 a         1385 c         1160 g          745 t
ORIGIN      
        1 gcggatgtgg tgtctcaggc ggtgtcggtt ttcggatctg ttcttcttga tcattggaag
       61 caaccctgac cgaacatgac ttacgctttg gcgccgctgg ctgcggcggc tttgcccgcc
      121 ttgcggcgga tgtactcgcc ttcgtatttc acgcctttcc ccttgtaggg ttcgggcggc
      181 cggaagctcc ggatcttggc cgccacggct cccaccaact gcttgtcgat gccgctcacg
      241 gtgatctggg tctgatcctt cacctcgatc cggatgccgg acgggaccgg atagatgatg
      301 gggtgggaat agcccagcga caggttcagg ttcggcccca tggtcgaggc ccggtagccc
      361 acgcccacca actccagttt ccgctcgaac cccttcacca cgcccgccac catgttggcg
      421 atcaggctcc gggtgagtcc gtgcagggcc ttgtgaaact tctcatccgt gggtcgcccg
      481 cagacgacct gtcggtcctt cacctcgacc cgcatggagg ggtgggccgt gaagctcagg
      541 gaccccttgg gcccctgcac gctgaccgtg tgatccttga cgtccacctt cactgcatcc
      601 gggatcggga ttggaagtct gccgatgcgc gacatggtgt ctcagctcca ggacctcagt
      661 cgaacagcct taccagacgt aacacagcac ttccccgccc acggaccgct cccgggcggc
      721 cttgccggtc atcaggccct gggacgtgga caggatcgcc acccccatcc cgctgaacac
      781 cctggggatc tgggcggcct tccggtacac ccggagaccc ggtcggctga cccgtttcag
      841 gccctggatg acccgttcgt tccccggtcc gaacttcagg tagacccgca ggatcccctg
      901 ccggctgtcg tcggcgagct tgtagccccg gatgaagccc tccgctttca ggaccttggc
      961 cagctccacc ttcagccggg aggccgggat gtccacccgc tccaattggg cctgattggc
     1021 atttcggatg cgcgtgagca tatccgcgat cgggtccgtc atcgacatgc gcgactccac
     1081 attcaggggt cagacttcac gtcccggcag cgcgctcgcc cccaggggac tccgcctacg
     1141 ctagccctac caggacgcct tcaagacgcc tgggatctcc ccgcgcaagg ccaggccgcg
     1201 gaagcagata cggcacatct ggaacttgcc catgtacccc cggggccggc cgcagatccg
     1261 gcagcggtgg tagcggcgca ccgcgaagcg gggcggtcgc tgctgcttga tgatgagact
     1321 cgtcttcgcc atgtcgcctc tcgcttactt tcggaagggg aacccgaggt gcgtgagcag
     1381 ggcccgggcc tgctcgtcat tccgggccga ggtctcgatg cacacatcca tgccccgggt
     1441 catgtccacc ttgtcgtacc ggatctcggg aaaaatgagc tgctcccgaa tgcccagggc
     1501 atagttcccg cgaccgtcga aggacttggc cggcacgccg cggaagtcgc ggatgcgcgg
     1561 cagggccacg gagagcaggc gatccaggaa ctcgtacatc cgctcacccc ggagggtcac
     1621 cttgcagccg atcggcatcc ccgcgcgcag cttgaaggcg gcttccgatt tccgggcccg
     1681 ggtgaccacc ggcctctggc ccgtgatggc cgccaattct tccaccgcgg cgtcgatcac
     1741 cttcacgttc cccaccgcct cccccaggcc catgttgatg acgaccttct tgacctcggg
     1801 gacctgcatc cgattcgcgt agccgaactc cgtcatcagg gcctgggtga tctcgccccg
     1861 gaatcgtgcc cggagacggg ccgggacccg cggaacctgc ggagcggctg ccgcctcggg
     1921 tttccccttg gcaccccgct cccccttggc gcccttctcc cccttggctg ccttcccagc
     1981 atcggcccgg gccttgcgtt ccgccttgtc cgccatggct caatccatcg tctcgccgca
     2041 ctcgcggcac acgcgcacct tccgcccatc cgccagttgg gtgcgcccga cccgcacggg
     2101 gcggtcgcac ttgttgcaca ccagctgcac gttggatacg tgcagggggt tctaccgttc
     2161 gaggatgccg ccctgctggc caaagcgtcc cggtttcgtg tgccgcttca cgaagttgat
     2221 cttctccacc aggatcttgt ccagcttggg caggacgcgc aggaccttgc cgcgcttccc
     2281 cttgtccttc cccgcgatca cctcgatcag gtcgttcttg ttgaccgcca gccgatgtcc
     2341 gacacccatg acgcgacccc gtgagacgtc cagccgaccc tggactgcct agatcacttc
     2401 cggcgccaac gagatgatct tcataaagcg ggcctcgcgg agctcccgcg ccaccggccc
     2461 gaagatgcgg gtcccgaccg gatcgttgtg gtcgtcgatg aggacggcag cgttgcggtc
     2521 gaacttgatg tagctcccgt ctggccgccg ctgctccttc acggtgcgga ccaccaccgc
     2581 cttgaccacc gtgcccttct tcaccgtccc ctcggggacc gcttccttca cgttggcgac
     2641 gatgatgtcc ccgatgtggg cgtagcgctt cgccgagccc cccaggacct tgatcaagga
     2701 gatgcgcttg gccccggagt tgtccgccac atccaggatg gttcgcaggc cgatcatggc
     2761 tctaccctgt cgtcctcagg tccgaaccgc ccccgcctcc ggcggagacg ctcagacggc
     2821 cctttgcagg atctccacca cccgccagcg cttgtccttg gagagggggc gggtctccat
     2881 gagctccacc ctgtcgccca catggcaggc gttctgctcg tcgtgggcct tgaccttggt
     2941 cttgcgccgg accatcttgt cgtaggactg atggcggacc agccgggcga tctccaccac
     3001 caccgtcttc tgcatcttgt cgctgacgac cattcccacg accgtctgtc gccggctgcg
     3061 gtcctccgtc atgttgggcg ccatgctaga ccccttcctt cgacgccgtc gtggcggcca
     3121 gggcgcgctg cgccgtctgg acgcgggcga tgtcgtggcg aagctgccgg atccgcatgg
     3181 ggttctccac ctgggtcgag gcctggcgca gacgcagctt gaagaggtcc tcacgcaacg
     3241 ctgcttcctt ctggcggagc tcctccgcag tcaggtcctt cagctcggca tacttcatca
     3301 catcgtcccc ccgactccgc gggtcacgaa gcgcgtggcg atggacagct tcatggctgc
     3361 caggcgcatc gcctccttgg cctcagcctc cgggatccct tccatctcgt agaggatgcg
     3421 gcccggtttt accacggcca cccagccctc cggggccccc ttacccttgc ccatgcgggt
     3481 ctccgccggc ttggccgtga tgggcttgtc cgggaagatg cggatccaga ccttgccgcc
     3541 ccgcttgacg tagcgggtca tggcgcgccg ggcagcctcg atctgccggt tcgtgatcca
     3601 ggccgcctcc atggccttca atccatactg accgaaggcc aggacgctcc cgcgggtggc
     3661 gatcccggac cgccgacccc gctgttgctt ccggaacttt acgcgcttgg gtaccagcat
     3721 gccgtgctcc tcgccctcgg gctaggtccg cgacgctggg ggtggcgtgc ccggcccccg
     3781 gcgccgccga gggcgatccg agggtgttcg gaggtcgcgg agacgctccg gcggcttgc
//
LOCUS       DBNGZC010000703         1895 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_159498, whole genome shotgun sequence.
ACCESSION   DBNGZC010000703 DBNGZC010000000
VERSION     DBNGZC010000703.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_159498"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1421..1834
                     /locus_tag="AC16G0_12135"
     CDS             1421..1834
                     /locus_tag="AC16G0_12135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5100396.1"
                     /translation="MLIVDDEASPRAVIREVLHRFGYESAEAGDGLDGLAKAEAVRPD
                     VILLEGRMRGLDGYEVCRRLKANTRTQHIPVIFVTGVEDDELYALADEVGAVASLTKP
                     FHFETLVAVIEAALTNAEPQTGPTIKSDEVKADSC"
BASE COUNT          376 a          531 c          661 g          327 t
ORIGIN      
        1 tcgaggtccc tcgaccccgt gggctgccga ttgcactggt cttgacccgg cgaccgagag
       61 gcgaatccag cccgaaagga atgatagaag gaagggaccc cagtgtcctg agggacagat
      121 tcgctgacaa gatctggcga cgctcgccag gggatcgtcg gtggtcttgg cccacctgta
      181 ccaagctgtc cgcctcggag aatccttcct ttgtcaagtt gaagggcacc ctcttggtcc
      241 tcaccgacgc ggatcatcga gccgggctcc gccggggaag tggcggaacg ccagatggta
      301 ctggcccagc ggaaggctac cgctccacgt ccgcctgtgg tagcatccag cggaaaacct
      361 gacaggaagc cctggtggga ggagatacgg aaaggacgct tggtccgtct ggacgaccgg
      421 ctgtccgttg tcctcgccgt gctccgggta ttttggaggt cacatgttca gcggtgacga
      481 ccgtagcctt tcgtcagcgg atgaccggcc ccctggtagc gagtcgccat cctgcccgcc
      541 tgcccctatc gcgccgtggt cgccttacgg ccggaggccg agatggtcgg gaccagcgtc
      601 ctagacttga cgcgttgaat atccaagggt ggtacatcct catgcggggc tttgtgcaca
      661 tttggggggt tccctttcct tgaaaggggg gaaaggagga gccatgaaga ggatggggcg
      721 gatggccctc gtgatcggaa tggtcggtgc cacgttagtg gccgggtgtg gctttcaccg
      781 ggagacgtgg cggaccacaa acgggcgggg ggcggtcagc gtcccgttgg gtcagttccg
      841 ttcgagtcca tatggcttcc aaagcaagag ggccaaggag gtcatggtcg ccaaatgtcc
      901 cggaggatac gccgtcctgc aggaaggcac cacaccgtca ggagaagatg cgcgtggact
      961 ctggagaaac ccacagcccg gggagggagg cattgcttat gagtggtaca agtacgaccc
     1021 gaaatggtac tgggagtatg aatgcaccag gtaagccccc atgacggtgt gcagcggaca
     1081 ggctccggat cccagctggt gagttagcct ttcgggaacc ggcggagtcg cgtgggtgac
     1141 agagccaaaa cggcgcgaag ccctcaggga catcatccag agcgtcatcg cgaacgtgga
     1201 gcgccgggcg aaacgccaaa gaaaagtcgg agggggtggt cgctcagctc ccgcgcccac
     1261 cgctcagacc gctgtcgggt gggcacttgg caacagaagc gagaggatga attgaacctg
     1321 ctggaggagt agctcacccg tgccttgacg cgggtacgag cctccgaggt gtgaaagcag
     1381 cagcgtaggg cgagtggtga ggaccttggc gagaaagcgc gtgcttatcg tggatgatga
     1441 agcgagcccc cgtgcagtca tccgggaggt actccacagg ttcgggtacg agtccgccga
     1501 ggctggagac ggcctggatg gtctcgccaa agccgaggcc gtccgcccag atgtcatcct
     1561 gctggagggg cgcatgcggg ggctcgacgg ctacgaggtg tgtcggcgcc tgaaggcaaa
     1621 cacacggact cagcacatcc cggtgatctt cgtgacggga gtggaggatg acgaactata
     1681 cgcgcttgcc gacgaggtgg gagccgtggc gtctctcacg aagccctttc atttcgagac
     1741 gctcgtggcc gtcatcgagg cggccctcac caacgcagag ccccagactg ggcccacaat
     1801 aaagtcggat gaggtgaagg ctgactcttg ctgagcgcct gctctgacac gtgccgggcg
     1861 gctgcctgac agcggcagag gggagacggc ctggc
//
LOCUS       DBNGZC010000704         2742 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2312768, whole genome shotgun sequence.
ACCESSION   DBNGZC010000704 DBNGZC010000000
VERSION     DBNGZC010000704.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2312768"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="AC16G0_12140"
     CDS             complement(<1..520)
                     /locus_tag="AC16G0_12140"
                     /inference="COORDINATES: protein motif:HMM:NF014766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enolase-like domain-containing protein"
                     /protein_id="HIR5100397.1"
                     /translation="MRITNIQCIPVECHLPQPVYDANYIMATKPALLVEVTTDEGLVG
                     LGEAAHFGGPLHSTRTVIEGELRDHLLGEDPRDVERLWEQMHQRAYKHGRGGILIAAM
                     SGIDIALWDLRGKMTGLPLWRLLGGYRRRVPAYATGGFYAEGKGIRELCAEMEAYCRH
                     GFTAVKMKVGRNS"
     gene            complement(748..1836)
                     /locus_tag="AC16G0_12145"
     CDS             complement(748..1836)
                     /locus_tag="AC16G0_12145"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HIR5100398.1"
                     /translation="MATHSIAVVKGDGIGPEVCTAAIEVIQSALGAGSRLEFCEYPAG
                     AAHYLRAGDAFPEETFEGCRKADAILHGAAGLPGVLLPDGTEAGPDFGLKIRSRLDLY
                     ANMRPIRLWPGLKSPLRRYPPGRIDYVVIRENTEGLYASRGAGAVLRDEVAADTLMIT
                     RKGTERVVRFAFELARKRPGAPRDGKRRVTACDKANVLRSYAFFRRVFNEVAAQYTEI
                     ATDYAYVDAMTVHQVNRPDFYDVIVAENIFGDIISDLGAATVGSMGMSPAAELGDHHG
                     FFQASHGSAPDIMGQGIANPLGTILAGSLMLKWLGERTQDPNLHQSAARIEQAVESVL
                     ASGKTLTQDLGGRATTAELTKAVCEALG"
     gene            complement(1895..>2742)
                     /locus_tag="AC16G0_12150"
     CDS             complement(1895..>2742)
                     /locus_tag="AC16G0_12150"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5100399.1"
                     /translation="AAVAMAYVSEEAPAAAAGQTMAAYVTGTVIGGFAGRFLNGLVAP
                     YWGWRTAFVLLGATTLLSASGTGWRLPPSRKFVPQGAGVASFRVLGKHLRNRQLLATY
                     LVGASVLLSQVSTFTYVNFYLADPPFRLGPTGLSMIFAVYLLGAAVTPAAGRLVDRLG
                     DRTVLLGAVGLGGLGILVTLLPVLVAVIAGLTLLDAGAFICQAAAQSHVGTVAGDARS
                     SAAGLYISLFYVGGTLGSVIPGLFWDVAGWAGCVAFILAVQVLIGGIAHRYWQRSPVE
                     PLVQS"
BASE COUNT          539 a          922 c          840 g          441 t
ORIGIN      
        1 ggctattccg tcccaccttc atcttgacgg ccgtgaagcc gtgccggcag tacgcctcca
       61 tctcggcgca gagctcccgg atgcccttcc cttcggcgta gaagcccccc gtggcatatg
      121 ccggcacccg gcggcggtac ccgcccagca ggcgccacaa ggggagtccc gtcatctttc
      181 cccgcagatc ccaaagggcg atgtcgatcc cgctcatggc agcgatgagg atgccgccgc
      241 ggccatgctt gtaagcccgc tggtgcattt gctcccagag tcgctccacg tcccggggat
      301 cctcccccag gaggtgatcc cgaagttcac cctcgatgac tgttcgcgtc gaatggagag
      361 gacccccgaa gtgcgcggct tcccctaatc caaccagccc ctcgtccgtg gtgacctcga
      421 ccagcagcgc tggcttggtc gccatgatat agttagcgtc gtacacgggt tggggcagat
      481 ggcattcgac cggaatgcac tggatgttcg tgattcgcat agaaattctc cctcctcttc
      541 gtcaggccgg ttacgccacg cgagtttgat catgcgatgg gcgcgatcgc gatgaagaat
      601 catcggctca cgcccccagc aacagcaacg cctcttgcct gtcgagtcga gccgcgccat
      661 gagatcgtgt cggctgattc tcaactggcg caaatctcga ctgcgttggg tcagccatac
      721 cccagaccat cgccgaggcc gaatccatca gccaagcgcc tcacacacag ccttggtcag
      781 ttcggcggtc gtcgcccgcc ctcccagatc ctgagtgagc gtcttgcctg aggcgaggac
      841 actctctacc gcctgctcaa tccgcgccgc agactggtgg agattcggat cctgtgtgcg
      901 ctcgcctagc cacttcagca taagcgagcc ggcgaggatc gttcccagcg gattggcgat
      961 accctggccc atgatatccg gggcagatcc atgcgacgcc tgaaagaagc cgtgatgatc
     1021 gccgagttca gccgcaggcg acatccccat gctccccacg gttgccgccc cgagatccga
     1081 gatgatgtct ccaaatatgt tctccgcaac aatcacatcg tagaaatccg ggcgattgac
     1141 ctggtggaca gtcatggcat ccacataggc atagtcggtc gcgatctcgg tgtactgggc
     1201 cgcgacctcg ttgaacacgc ggcggaagaa ggcgtagctc cgcaagacat tcgccttgtc
     1261 gcaggcggtc acccgccgct ttccatcccg cggggcgcca ggccgctttc gagccagctc
     1321 gaaggcgaat cggaccaccc gctcggttcc tttccgggtg atcatcaagg tatcggccgc
     1381 gacctcgtcg cgcaagacag ccccggctcc ccgcgaagcg tacagcccct ccgtgttctc
     1441 gcgaatgacc acgtagtcga ttcgaccggg tgggtagcga cgcaacgggg atttgagtcc
     1501 cggccagagg cgaatgggcc gcatgttggc gtacagatcg aggcgtgagc ggatcttcag
     1561 gccgaaatcc ggtcccgctt cggttccgtc tggaaggagg acgcccggca ggcccgcagc
     1621 gccatggagg atggcgtccg ccttccggca tccctcgaac gtctcctcgg ggaaagcgtc
     1681 accggccctc aggtagtgcg cggcacccgc ggggtactcg cagaactcaa ggcggctccc
     1741 ggccccgagg gcggattgaa tcacttcaat ggccgccgtg cagacttcgg gcccgatgcc
     1801 gtcccccttt acaaccgcga tcgaatgcgt ggccatggtc gtagctccca ggagaagcgc
     1861 actgcgacac caagcctgag cagaacaatc caaactagga ctgaaccaat ggttccacgg
     1921 gagaacgctg ccaatacctg tgagcgatcc cgccgatcag cacctggacc gccaggatga
     1981 aggccacaca ccccgcccag cccgcgacgt cccagaacag gcccgggatg acggagccca
     2041 gggtcccccc gacgtagaag agagagatgt agaggcctgc cgcagaggag cgggcatccc
     2101 ccgctaccgt tccgacgtgg ctctgggccg ccgcctggca gatgaaggcg cccgcatcca
     2161 agagagtgag gcccgcgatc acagccacga ggacgggaag gagtgtgaca agtatcccca
     2221 ggccccccaa accgaccgcc ccgagcagca cagtgcggtc tcccagccga tccaccagcc
     2281 ggccagccgc gggggtgacg gcagccccga gcagatagac cgcgaagatc atggatagtc
     2341 cagtggggcc gagtcgaaag ggcggatcgg ccaggtagaa gttgacgtag gtaaaggtgg
     2401 agacctgcga gaggagcacg gaagcaccca cgagataagt ggccaagagc tgccggttcc
     2461 gcaggtgctt tcccaggact ctgaacgagg cgaccccggc accctgcggc acgaatttcc
     2521 tggagggcgg cagccgccag cccgtcccgg aggcgctcag aagggtcgtg gcccccagaa
     2581 ggacaaaggc cgtgcgccaa ccccagtacg gggcgacgag accgttcaga aagcgacctg
     2641 caaagccgcc gatgacggtg ccggtgacat aggccgccat ggtctgtccc gctgccgcgg
     2701 caggagcctc ctcgctgacg taggccatgg ccaccgccgc ga
//
LOCUS       DBNGZC010000705         2136 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_256224, whole genome shotgun sequence.
ACCESSION   DBNGZC010000705 DBNGZC010000000
VERSION     DBNGZC010000705.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_256224"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..1174)
                     /gene="folD"
                     /locus_tag="AC16G0_12155"
     CDS             complement(314..1174)
                     /gene="folD"
                     /locus_tag="AC16G0_12155"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HIR5100400.1"
                     /translation="MSARTIDGKKIAAVVREEIRLRVLQYKKTTGRVPGLAAVLVGDD
                     PASASYVRSKTKACQEAGILSRQLSPPGDISQAELLALIGELNAEPNIHGILVQLPLP
                     TTLNERAILESVNPDKDVDGFTFGSIGRLVENQPGFVPCTPAGILELLDREGVEISGK
                     HAVVIGRSEIVGKPVALLLLHRHATITICHSRTPDLAAETRRADILVAAVGRPRLITG
                     SMVKPGAVVIDVGINRVEGRLVGDVDFESAVQVASAITPVPGGVGPMTVAMLLKNTLR
                     AFEHSSAISP"
     gene            complement(1281..2057)
                     /locus_tag="AC16G0_12160"
     CDS             complement(1281..2057)
                     /locus_tag="AC16G0_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012545500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HIR5100401.1"
                     /translation="MRILFIGDIVGQPGRRMVARHLKELVAEHQADVVLANAENAAAG
                     FGVTPSVGEELFSLGIDLLTSGNHVWDKKEAEGYIAKESRMLRPANYPEEAPGTGVHI
                     LRKQDRLVGVLNLQGRAFLPAIDCPFKAADREIPHIQQVTDIILVDFHAEATAEKQAF
                     GWYVDGRVSAVLGTHTHVQTADERILPERTAYITDVGMTGPRESVIGIRREDAIQKFI
                     TQMPKKFNVADGATQLSAVVLDIGDDGRARRITRLQIQDN"
BASE COUNT          370 a          706 c          657 g          403 t
ORIGIN      
        1 gggttccttg cacgcttcga gggcctgctt cagactgagg tgaaactcca ttggcatggc
       61 tccccctggc ttccctgcgg ggcgggaggg acacctcccc tcgcctcctc cccttgagcg
      121 caggttagga tttgctcacg gccacatagg ctgccccgga aagacgtgag ccccttgagg
      181 gagaagtccc cccaaagggc ttcgcgccaa cgggtagcat gctcatcttc gcccggcata
      241 gaagtcctca ccaggctggt atgaggtaga ggctgcggat cctgagcgac gctatcgcca
      301 ccggaggggc aagtcaagga gaaatggctg acgagtgttc gaaggcacgg agggtgttct
      361 tgaggagcat agccacggtc atgggcccga cgccgccagg cacgggagtg atggcggagg
      421 caacctggac cgcggactcg aagtccacgt cgcctacgag tctcccctcg acgcgattga
      481 tcccgacgtc aatcacgact gcccccggct tcaccatcga ccccgttatc agccggggtc
      541 ggcccaccgc ggctacgaga atgtcggccc gacgcgtctc ggcggcgaga tcgggagtgc
      601 gcgagtggca gatggtgatc gtggcatggc ggtgaagcag gaggagggcc accggcttac
      661 cgacgatttc gctccgaccg atcaccaccg cgtgcttccc actgatctcg acgccctccc
      721 gatccaggag ttcaaggatg ccagccggcg tgcagggaac aaaccccggc tggttctcca
      781 cgagccttcc gatactcccg aaggtgaacc cgtccacatc cttgtcgggg ttcacggatt
      841 ctagaatggc tcgctcgttc agggtggtcg ggagaggcaa ttgcaccagg ataccgtgga
      901 tgttgggctc ggcgttcagt tccccaatca gcgcaagcaa ttcggcctgg gaaatgtcgc
      961 ccggcgggct gagctgtcga gataagatgc cggcctcttg gcaggctttc gtcttggacc
     1021 ggacgtagga agccgatgcc gggtcgtcgc cgacgagcac ggcggccagg cccggtaccc
     1081 tccccgtcgt cttcttgtac tggaggaccc gcaggcgaat ctcctcccga accacggccg
     1141 cgatcttctt cccgtcgatg gtccgggcac tcacggcatg cccctccgct cccctgcgcg
     1201 accccgctgc ctcccccttg cctcgccgaa tggggtccga cggggacagg acagcggcat
     1261 gcgcgactcc ccaccccagc ttagttgtcc tggatctgaa ggcgcgtgat gcgtcgagcg
     1321 cgcccgtcgt ccccgatgtc cagcaccacc gccgagagtt gcgttgcccc atcggctacg
     1381 ttgaatttct tcggcatctg ggtgatgaac ttctggatag catcctcccg tcggatgccg
     1441 atgaccgact cccggggacc cgtcatgccc acgtccgtga tgtaggcggt gcgctcgggc
     1501 aggatgcgct catctgcggt ctgcacgtgg gtgtgggtgc ccagcacggc cgaaacgcgc
     1561 ccgtccacat accaaccgaa ggcctgtttc tcggcggtcg cctcggcatg gaaatccacc
     1621 aggatgatgt cggtgacttg ctggatgtgg ggaatctctc ggtccgccgc cttgaaggga
     1681 cagtcgatgg ccgggaggaa tgccctccct tggaggttga gcacccccac caggcgatcc
     1741 tgttttcgca ggatatggac accggtgccc ggagcttcct cgggatagtt cgccggccgc
     1801 agcatacggg actctttggc gatgtatccc tcggcctcct tcttgtccca gacgtgattg
     1861 ccggaggtga gcaggtcaat ccccagactg aagagctctt ctccaaccga gggggtgacg
     1921 ccgaaccccg ccgccgcgtt ctccgcgttg gccaggacca catccgcctg gtgctcggcc
     1981 accagttcct tcagatggcg cgccaccatg cggcgccccg gctgcccgac gatatccccg
     2041 atgaacagga tgcgcatctc aactattcga gcttcgaact tcgaaattcg aaattggcct
     2101 cggattcaca cccctgctgc taccgcgcat attcca
//
LOCUS       DBNGZC010000706         2721 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_357410, whole genome shotgun sequence.
ACCESSION   DBNGZC010000706 DBNGZC010000000
VERSION     DBNGZC010000706.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_357410"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            498..1430
                     /locus_tag="AC16G0_12165"
     CDS             498..1430
                     /locus_tag="AC16G0_12165"
                     /inference="COORDINATES: protein motif:HMM:NF013247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR5100402.1"
                     /translation="MADYTAYFCGQWMPASQVKIDPMDRGFLVGDAVFDVARTFNGKS
                     FRMREHVDRLYRSLRYVRIDPGLSPEAMLEISEEVIRRNEHLRAEVGDFAIWQFVTRG
                     RGRWAHSAGPPSVGVFIRSLGLSRFVHLYSEGAHGVIVRVRSYSPESLDPKVKNFSRM
                     NFNIAELEAADIDKAGWPILLDHRGNIAEGVGYNVFLVTDGVIRTPGDRSILQGVSRR
                     MVFDLAKGLGIPIAEEDLQPYDLYTADEAFFSSTSPCVLPVTRVDRRPIGNGVPGPIV
                     HRLLAGWSEAVGVDIVEQARIYGPKEQLESKASS"
     gene            complement(1727..2563)
                     /locus_tag="AC16G0_12170"
     CDS             complement(1727..2563)
                     /locus_tag="AC16G0_12170"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5100403.1"
                     /translation="MLRSILVPVGRLSLYLVVGGVGALMILPFYWMIITALMPAPDIL
                     SFPPKWFPTTVTLEHFVAAFAKAPWLTYYWNSLVVAVGSVSCSLFFGLFAGYAFAVYR
                     FPLQNFFFLLILGTLMVPVQVTSVALYIQLSKIGWVDTFLGILAPNFASAFGVFMLRQ
                     GIKQIPVDVIEAARIDGAGEMRIVLTIIAPLIKTTVATVAMILFLGSWNDFLWPVIII
                     NSADLRTLPVGIALFKDPYGNLNYGPLMAATLITTAPMLVAYVISQKYMIRGIAMTGL
                     KG"
BASE COUNT          541 a          810 c          881 g          489 t
ORIGIN      
        1 tgtggagagg ttgaccctgc gggtctcgtc gtcctccctt ccccggagac atccggaaaa
       61 ccagctccca gggcgagagc ctgcccgtgt cattgacgtg ggtcacccgg agcagaggac
      121 accgatgccg ctccggggag atcctccgcc ctgcattgcg accgacattc gatgatggac
      181 accacattct tcgagttgtt cgagggggaa tggcgaggtt ctgcaacggg tagaattgat
      241 cgttccagcc aataccaaag ccacgcgcac acgggcgaag agttttcggc tggttgcctg
      301 gtcacgtgac ggcagtctag caccctagac cgggggggtc aaagagaggc tggggcactc
      361 agccatcacg aacagtcgag cccccgactg gggcgggagt gcgccttgcg tctcctaggc
      421 gtgcaatgta tagtgcctga gacggaagcg aagggttccc caggagcgca cgagttcacg
      481 cgcgggagga gaggggcatg gcagattata cagcgtactt ctgtgggcaa tggatgcccg
      541 ccagccaggt caagatcgat cccatggacc ggggattcct ggtgggtgac gcggtgttcg
      601 atgtggcccg caccttcaac gggaagagct tccgcatgcg ggagcacgtg gatcggctct
      661 accggtccct gcggtacgtc cgcatcgatc ccggtctctc gccggaggcc atgctggaga
      721 tcagcgaaga ggtcatcagg cggaacgagc acctgcgggc cgaggtgggc gactttgcga
      781 tctggcagtt cgtgacccgc gggcgaggac gctgggctca cagcgccggc ccccccagcg
      841 tcggcgtgtt tatccgatcc ctgggtcttt ctcggtttgt ccacctctac agcgaagggg
      901 cccacggcgt catcgtccgc gttcggagct actcgcctga gtcgctggat cccaaggtca
      961 agaacttcag ccggatgaac ttcaacatcg ccgaactcga agccgccgat atcgacaagg
     1021 ccggctggcc gattctgctg gatcaccggg gcaacatcgc cgagggggtg gggtacaacg
     1081 tgtttctggt caccgacggc gtcattcgca ccccggggga ccgctcgatc ctccaggggg
     1141 tatcccggag gatggtcttt gacctggcca agggcctggg tatacccatc gcggaggaag
     1201 atttgcagcc ctacgacctc tacacggccg acgaggcctt cttctcgagt accagtccgt
     1261 gtgtcttgcc ggtgacccgg gtggaccggc gccccatcgg caacggtgtt cccggcccca
     1321 tcgtccaccg gcttctggcg ggctggagcg aggccgtggg ggtggacatc gtggagcagg
     1381 cgcggatcta tggcccgaag gagcaactcg aatccaaggc cagctcgtga caggctgctg
     1441 aaagaggccc atctgctgcg ttggcgcgcc tcgccactgg ctgcggcgta catgcagtac
     1501 gcctggaccc agcagcgctg ggtaccccgc ctacggcgcg ccgccttgca tctggacctg
     1561 tttgagcagc ctgggagaag gcgggtattt cagcggcccg ctaattcctg aagagcttcg
     1621 atacaacaaa gggcggcatc ccccacgaca ggggatgccg ccctcttcct tgtatctgcc
     1681 gatcctctcc cgatttccgg cctctgtccc ccgatccccc cgtcccttac cccttcagcc
     1741 ccgtcatggc gatgccacgg atcatgtact tctgggagat gacgtaggcc acgagcatgg
     1801 gcgccgtggt gatgagcgtc gccgccatca ggggcccata attgaggttg ccgtacgggt
     1861 ctttgaagag ggcgatgccc accggcagag ttcgcaggtc tgcagaattg atgatgatga
     1921 ccggccagag gaaatcgttc caggagccca ggaacaggat catggccacg gtggcgaccg
     1981 tcgtcttgat gaggggagcg atgatggtga gcacgatacg catctcgccg gccccgtcga
     2041 tccgggcggc ctcgatgacg tccaccggga tctgcttgat cccctggcgg agcatgaaga
     2101 ccccgaaggc gctggcaaag ttgggtgcca ggattccgag gaaggtgtcc acccagccga
     2161 tcttggagag ttggatgtac agggccaccg aggtgacctg gacgggcacc atcaaggttc
     2221 ccaggatgag gagaaaaaag aagttctgca aggggaagcg atagacggcg aacgcgtagc
     2281 cggcgaacag gccgaagaaa agcgagcagc tcaccgatcc gactgcgacg acgaggctat
     2341 tccagtagta ggtcaaccag ggcgccttgg cgaaggcggc gacgaaatgc tccagggtca
     2401 cggtggttgg aaaccacttg ggaggaaagg agaggatgtc cggcgcgggc atcaaggcgg
     2461 tgatgatcat ccagtaaaac gggaggatca tgagcgcgcc caccccgccc accacgaggt
     2521 acagactcaa ccttcccacc ggcaccaaaa tggaacggag catctcttcc tcagtccgga
     2581 ttactcgcga acgcattcat aagaattccg gggcaatgac cgaatcccaa tggcggatag
     2641 gatgtccgtg atatcccttg acggcgcgcg gcgcacgctt ttcaccattt gtcactggaa
     2701 attggtcatt gtcaatacgc g
//
LOCUS       DBNGZC010000707         1356 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2016310, whole genome shotgun sequence.
ACCESSION   DBNGZC010000707 DBNGZC010000000
VERSION     DBNGZC010000707.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1356)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2016310"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="AC16G0_12175"
     CDS             <1..364
                     /locus_tag="AC16G0_12175"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5100404.1"
                     /translation="AIRSLPAIAIAYAASKAAVIALTREIAIQYAAAGIRANAILPGL
                     MDTPMVEAQLTTAYGGEVEEMRRRRARLSPTGKQGDAWDTAHAALFLASDEAKYINGT
                     TLTVDGGLTVQVVPQAAS"
     gene            457..>1356
                     /locus_tag="AC16G0_12180"
     CDS             457..>1356
                     /locus_tag="AC16G0_12180"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR5100405.1"
                     /translation="MAEDTSLQLAMFEQMLTIRRFEEKAIELFEKGLIRGNVHPCIGQ
                     EAVSVGVCAILRRDDYMTSTHRGHGNCLAKGADPRLMMAELLGRETGYCKGKGGSMHI
                     ADFEGGNLGANGIVGGGLPIATGAGISIQNRETDQVVVCFFGDGAANQGTFHESLNLA
                     ALWKLPVVYVCENNQYVLSTPLRESVGLPEISERARAYGITGVRVDGNDVLEVHAKAG
                     EAVRRARAGEGPTLLDCLTYRFFGHFTGDRGHGITYRSKEEMEEWRGRCPIARLRKRL
                     LANGALTEADAAAIEAREDSAIAA"
BASE COUNT          246 a          415 c          485 g          210 t
ORIGIN      
        1 tgcgatccgg agtctcccgg cgatcgcgat cgcctacgcg gcctcgaagg ccgccgtgat
       61 cgccctcact cgggaaatcg ccatacagta tgccgccgcg ggcatccggg cgaatgccat
      121 cctgcccggt ctcatggaca ccccgatggt ggaggcgcag ctcaccaccg cctacggggg
      181 cgaggtggag gagatgcgga gacggcgagc ccggctgagt cccacgggta aacagggaga
      241 tgcctgggac acggcccacg ccgccctctt cctggcatcg gacgaggcca agtacatcaa
      301 tgggacgacg ctgacggtgg acggggggct gaccgtccag gttgttcccc aggccgcgtc
      361 ctgacccgga ggattcagcg cgcgaatgat cctgagccac gggcgtcttg gcggcggaat
      421 gccggcacgg ggccattcac gtagggggtc tctctcatgg cagaggatac gtcgttgcag
      481 ctcgcgatgt ttgagcaaat gctgaccatc cggagattcg aggagaaggc catcgagcta
      541 ttcgagaagg gcctcatccg cgggaacgtg cacccctgca tcgggcagga agccgtctcc
      601 gtgggggtct gcgccattct gcggcgagac gattacatga cgagcaccca tcgcggccac
      661 ggcaactgcc tggccaaggg agcggatccg cggctgatga tggcggagct tttgggacgg
      721 gagaccggct actgcaaagg caaaggcggc tccatgcaca tcgcggattt cgagggcggg
      781 aatctgggtg ccaacggcat cgtggggggc ggcctcccca tcgcgaccgg ggccggcatc
      841 agcatccaga accgggagac ggatcaggtg gtggtgtgct tcttcgggga cggggcggcg
      901 aaccaaggga cctttcacga gagcctcaac ctggcggccc tctggaagct gccggtcgtc
      961 tacgtgtgcg agaacaatca atatgtgctg tccacgccgc tccgtgaatc ggtcggcctt
     1021 cccgagatct ccgagcgggc ccgggcctac gggatcaccg gcgtccgcgt ggacgggaac
     1081 gacgtcctgg aggtgcatgc caaggcgggg gaggccgtcc ggcgggcgcg ggccggagaa
     1141 gggccgaccc tgctcgactg cctcacatac cgattcttcg gtcacttcac cggagaccgc
     1201 gggcacggga tcacgtatcg ttccaaggaa gagatggaag agtggcgggg gcgctgcccc
     1261 attgctcggc tgcgaaaacg gttgctggca aatggcgcgc tgaccgaggc ggatgcggcg
     1321 gccatcgagg cacgggagga ttccgccatc gccgcc
//
LOCUS       DBNGZC010000708         4640 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_745138, whole genome shotgun sequence.
ACCESSION   DBNGZC010000708 DBNGZC010000000
VERSION     DBNGZC010000708.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_745138"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..162
                     /locus_tag="AC16G0_12185"
     CDS             <1..162
                     /locus_tag="AC16G0_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HIR5100406.1"
                     /translation="LTDLLQGTDIVKYGEVMGRATQPIPAGSHVHVHNIESLRGRGDL
                     EAREVGHGI"
     gene            152..1312
                     /locus_tag="AC16G0_12190"
     CDS             152..1312
                     /locus_tag="AC16G0_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013253705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HIR5100407.1"
                     /translation="MEFKGYRRADGRVGTRNYVGVLSTVVCANEVAERIVREVHGATA
                     FLHHQGCGQMPTDVERVTQALIGLGRNPNLAAVLLVSLGCESVAVEEIAHGIAASGKR
                     VERLGIQEEGGMARSVAAGQLLVQEMVMEASTLPAGACPASDLVLGLKCGSSDTTQGL
                     FANPAVGIASDLLVAAGGTSVLGEVTEFIGAEHILVRHARTPDVAQQILHLVQRMEAR
                     AKAVGCDMRGGQPTGGNIKGGLSTIEEKSLGAIAKAGVSQIQAVYEYGERPKVKGLVV
                     MDSPGREPEILTGLAAAGCTLIAFATGRGAPQGFPFVPVIKITGNKVTWEKLRDHMDF
                     YVGTIMEGTETLPTAGKRLFAEFMEFASGRLTKAEICGYINSMDIYVTGPVI"
     gene            1439..1870
                     /locus_tag="AC16G0_12195"
     CDS             1439..1870
                     /locus_tag="AC16G0_12195"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR5100408.1"
                     /translation="MQRLPLRNQTPVVLAIVLALSLAVDPALLAGSGFTDVSVDQARE
                     LMQRRAGQRDFVILDVRTPEEFAEGHLSGAVNMNVLASDFAARLATLERGKTYLVYCR
                     TGNRSTKAVQAMERLGFQSVHHMFEGIIGWQKKGFPISRTQ"
     gene            1931..2524
                     /locus_tag="AC16G0_12200"
     CDS             1931..2524
                     /locus_tag="AC16G0_12200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5100409.1"
                     /translation="MEKPYRKDLSHVTWDEVFARQVKRAGLVEEWMNDLQLKPGARVL
                     DVGSGPGYVSLVLAERVGPAGLVYAVDRKAEALAYLERLQRERGIAHIQRVVADATRM
                     NPGSVSPDSALVTMVLHHTDDPPGILANVARLLPTGALTVVAEFHPDGPCKVGPPRTE
                     RVAPEQVQAWCEAAGFKVLRYRRQTPEHYTWVVERSP"
     gene            2741..>4640
                     /locus_tag="AC16G0_12205"
     CDS             2741..>4640
                     /locus_tag="AC16G0_12205"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HIR5100410.1"
                     /translation="MYEIRLLDPATRDVPLRRDVRRLGNLLGCVLREQAGHALFEKEE
                     EIRNLTKDLRQSFSLEREAAMDRITEELDLPTATQIIRAFTTFLQLVNVAEQIHDIRK
                     LRYDQLTRPGIPGPGSLEHAVGHCKAAGLSAQALQTLLREMDITLVLTAHPTEAKRRS
                     ILEKTRRISRHLAALDHPLLTPGEQEVLEGEILADITAIWQSDEVRLRPPTVLDEVRS
                     GLFYFDEILWEVLPLLYRDLRHALAANYPGETFVVRPCLRFGSWIGGDRDGNPHVTLE
                     TTWEALRLHKELALRKHREGLKRLGGVLSQSTTQVGVSDELRASVETDEAQLGVAQSA
                     ADPRTEREVYRRKLRCMRLRLEATLAANARGGRNSKPEPARPYSRAVELLADLAIVQQ
                     SLAENRGERLAAGAVQTLVDQVGVFGFHLASLDIRQDASVLRACVAEIAQAVRLADGD
                     IRDLHETERQTLLTRELRTLRPLIPPYARWSAETADTIELFRLIRRARAEVAPEAITA
                     FILSMTEQPSDVLAALLLAKEVGLLTREVGGLVSQIDLVPLFEKIHVLQAAPGVMDTL
                     YANGPYREHLAARGGIQEIMLGYSDSSKDGGYLTSNWELYVAHEALAVGATAQQVCLR
                     LFHGRGGTV"
BASE COUNT          831 a         1446 c         1589 g          774 t
ORIGIN      
        1 ttgaccgacc tcctgcaggg tacggacata gtcaagtatg gcgaagtcat gggccgggcg
       61 acacagccga ttcccgccgg gagccatgtg catgtccaca acatcgaaag cctccggggg
      121 cggggcgatc tcgaggcaag agaggtcggc catggaattt aagggctacc ggcgggcgga
      181 tgggcgggtg ggcactcgga attatgtggg ggtgctctcg accgtggtct gcgccaacga
      241 ggtggccgag cggatcgttc gggaagtgca cggggccacg gccttcctcc accaccaggg
      301 gtgtggccag atgccgaccg atgtggagcg ggtgactcag gccctgatcg gtctcgggcg
      361 gaacccgaat cttgcggcgg tgctcctcgt cagcctcggt tgcgagagcg tggcggtgga
      421 ggagattgcg cacggaatcg cggcatcggg aaagcgcgtc gaacggctgg gcatccagga
      481 ggagggcggg atggcccggt cggtcgctgc gggccagctt ctcgtccagg aaatggtgat
      541 ggaggcttcg accctgcccg ccggtgcgtg ccctgcttct gatctggtgc tcggcctcaa
      601 atgcggaagc tccgatacca cccaggggct cttcgccaac ccggcggtgg gcatcgcctc
      661 ggacctcctg gtggcggccg gggggacgtc ggtcctgggg gaggtcaccg aattcatcgg
      721 ggcggagcac atcctggtgc ggcacgcgcg gacgccggac gtggcacaac aaatcctcca
      781 cctcgtccag cgtatggagg cccgtgcgaa ggccgtgggc tgtgacatgc gcggggggca
      841 gcccaccggc gggaacatca aggggggtct ttcgaccatc gaggaaaaat cgttgggggc
      901 gattgccaag gcgggcgtct cgcagatcca ggcggtctac gagtatgggg aacgaccgaa
      961 agtcaagggc ctcgtggtga tggactctcc cggccgggag ccggagattc tcaccggatt
     1021 ggccgccgca ggctgcaccc tcatcgcctt cgccacgggc cggggtgccc cgcagggctt
     1081 cccgttcgtc ccggtgataa agatcaccgg aaacaaggta acctgggaga agcttcgcga
     1141 ccatatggat ttctacgtcg ggacgatcat ggaggggacc gaaaccctcc cgacggcggg
     1201 caagcggctc ttcgccgagt tcatggagtt cgcttcgggg cgactcacca aagccgagat
     1261 ctgcggctac ataaattcca tggacatcta cgtgacggga ccggtgatct gagcgggcag
     1321 tggatcagtt caccctcacc gcgagaggga gacagaagct tcgatgcagg aatacgagat
     1381 ccaagggaaa ccgagtggtt ggaagaacgc tggcaattct cgtccgtgag gtggcgacat
     1441 gcaacgactt ccattgcgaa atcagacacc cgttgtgttg gccatcgtgc tggcgctgag
     1501 cttggcggtt gatcccgccc ttttggccgg ctccggattc acggatgtgt cggtggatca
     1561 ggcccgggag ctgatgcagc gaagagcagg ccagcgtgat tttgtcatcc tggatgttcg
     1621 cacccccgag gagtttgccg aggggcactt gtccggggcg gtgaacatga acgtcctggc
     1681 ctcggacttt gcagcccggc tggccacgct tgagcgcggc aagacctacc tggtgtactg
     1741 ccgaaccggc aatcgctcca ccaaagccgt ccaggccatg gagcggctgg ggtttcaatc
     1801 ggtgcaccac atgttcgaag gcatcatcgg ctggcagaag aagggcttcc caatttctcg
     1861 aacacagtga gccggcagtc aaccgcgacc tcaggcgtca gataccacgc tattgtagcg
     1921 agggtgttgg gtggagaaac cgtatcggaa ggacctgagc catgtcacct gggacgaggt
     1981 gttcgcccgg caggtcaagc gtgccggcct ggtcgaggag tggatgaacg atctgcaact
     2041 gaagccgggc gcccgcgtgc tggacgtcgg ctccggcccg gggtatgtga gcctggtcct
     2101 cgccgagcgg gtggggccgg ccggactcgt gtatgcggtg gacaggaagg ccgaagccct
     2161 ggcctacctg gaacgcctgc agcgggaacg cgggattgcc cacatccagc gagttgtggc
     2221 ggatgccaca cgcatgaacc ccggaagcgt ctctcccgat tcggccctgg tgacgatggt
     2281 cttgcatcac acggatgatc cccccggaat tctggcgaat gttgcccgcc tgctccccac
     2341 gggagcgctg accgtggtgg cggagtttca tcccgacggc ccgtgcaagg tggggccgcc
     2401 ccggacggag cgcgtcgccc ccgagcaggt gcaggcgtgg tgcgaagcgg ccggattcaa
     2461 ggttctgcgc taccggcggc aaaccccaga acactacacc tgggtggtcg agcggagtcc
     2521 ataggcctcc cgccgccagt gacgagttcc cgcagatctt ctttcagatc acccgcattc
     2581 cggttcaatt caccacgccc gagggcacgc cgcgcctgac gggggcgtcc cgcaagaagc
     2641 cttgcggtct tccggtaggt cctctataaa gagagcctct tccggcgacc cgttgcacgg
     2701 gaagggacgc aacgggtacc ccggcagttc cggagtgggc gtgtacgaga tcaggcttct
     2761 cgatcctgcg acgcgagacg ttccccttcg gcgggatgtc cgacgcctgg gcaacctttt
     2821 ggggtgcgtc cttcgcgaac aggccggtca tgcgctcttc gagaaggaag aggagattcg
     2881 gaacctgacg aaggatctcc gacagtcctt ctccctggaa cgggaagcgg ccatggaccg
     2941 catcaccgag gagctggacc ttccgaccgc cacccagatc atccgggcct tcaccacgtt
     3001 cctacaactc gtcaatgtcg ccgagcagat ccacgacatc cggaagctcc ggtacgacca
     3061 gctcacacgg cccggtattc ccggccccgg gtcgttagaa cacgcggtgg gtcactgcaa
     3121 ggccgccggt ctctcggccc aggcgctcca gaccctgctc cgcgagatgg acatcaccct
     3181 cgtgctcacg gcccacccca cggaggcgaa gcggcggagc atcctggaaa agacgcggcg
     3241 gatctcgcga catctggccg ctctggatca cccgctcctc acgcccgggg agcaagaggt
     3301 cctggagggg gagatcctgg ccgatatcac ggccatctgg cagagcgatg aggtccggct
     3361 ccgccctccc acggtgctag acgaggtccg aagcggtctc ttctacttcg acgaaatcct
     3421 ctgggaggtg ctgcccctgc tctatcggga tctccgccac gccttggccg caaactatcc
     3481 gggtgagacc ttcgtcgtgc gcccctgtct gcggttcggc tcgtggattg ggggcgatcg
     3541 ggacggcaat ccccacgtga ccctcgagac cacctgggag gccctgcggc tgcacaagga
     3601 gctggccttg cgcaaacacc gcgaagggct gaagcggctc gggggcgtcc tcagtcagtc
     3661 cacgacacag gtcggggtaa gcgatgagct tcgggcgtcg gtcgagacgg atgaggccca
     3721 actgggcgtg gcacaatcgg cggcagaccc gcggacggag cgcgaagtgt atcgcaggaa
     3781 gctccgctgc atgcgcctcc gcctggaggc gaccctggcc gccaacgcga gaggagggag
     3841 aaactccaag ccggaaccgg cgcgacccta ttcccgagcg gtagaactgt tggcggacct
     3901 ggcgatcgtc caacagagcc tcgccgaaaa ccggggcgag cgccttgcgg cgggagcggt
     3961 ccagaccctg gtggatcagg tcggcgtctt cggcttccac ctggcgtcgc tcgacatccg
     4021 ccaggatgcg tccgtgctac gcgcctgcgt ggccgagatt gcgcaggccg tgcgcctcgc
     4081 ggacggggat atccgcgact tgcatgagac ggaacggcag accctgctca cccgcgagct
     4141 gcggacactg cggcccctca tccctcccta cgcgcgctgg agtgccgaga ccgccgacac
     4201 catcgagttg ttccgactca tccggcgggc gcgcgccgag gtggctccgg aggcgatcac
     4261 cgcctttatc ctgagcatga cggagcagcc gagcgacgtt ctggcggcgc tcctcctggc
     4321 aaaggaggtc ggcctcctga cgcgggaggt gggcgggctc gtgagccaga tcgacttggt
     4381 cccgctcttc gagaagatcc atgtgctgca ggccgccccg ggcgtcatgg acaccctgta
     4441 cgccaatggt ccgtaccgag agcacctggc cgcgcggggc ggaatccagg agatcatgct
     4501 ggggtattcc gacagcagca aggacggggg atacctgacc tccaactggg agctgtacgt
     4561 agcccacgag gccctggcgg ttggcgcgac ggcgcagcag gtgtgcctgc gactctttca
     4621 cgggcggggc ggcaccgtcg
//
LOCUS       DBNGZC010000709         4038 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_160434, whole genome shotgun sequence.
ACCESSION   DBNGZC010000709 DBNGZC010000000
VERSION     DBNGZC010000709.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_160434"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="AC16G0_12210"
     CDS             complement(<1..633)
                     /locus_tag="AC16G0_12210"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR5100411.1"
                     /translation="MTNLMLITLFGGMALLLYGMQLVGEGLQQAAGGKMRQILSSITN
                     NRFKGMMVGAFITAVIQSSSATTVMLVGFASSGLMGLPQTIGVILGADIGTTVTVQLI
                     ALEIFDYALLLVGVGFLLIFACRRKIFRYVGQAILGFGLIFFAMKLMSDAMVPLRQSE
                     LVKTLLVSLGDQPLLGLIVAAIFSALVHSSAATIGLAITLSLQGLLPLTAA"
     gene            complement(839..1474)
                     /locus_tag="AC16G0_12215"
                     /pseudo
     CDS             complement(839..1474)
                     /locus_tag="AC16G0_12215"
                     /inference="COORDINATES: protein motif:HMM:NF013046.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
     gene            complement(1490..1720)
                     /locus_tag="AC16G0_12220"
     CDS             complement(1490..1720)
                     /locus_tag="AC16G0_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100412.1"
                     /translation="MQAAAISAITGIFYHPSFSRRSYLTAGRRLADFPAALGALRQHP
                     LVRLEECPAATNEQILRVHASALVPKVEADPF"
     gene            complement(1757..2296)
                     /locus_tag="AC16G0_12225"
     CDS             complement(1757..2296)
                     /locus_tag="AC16G0_12225"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5100413.1"
                     /translation="MHDWEQELALQLQTVEGKQFSEQQAEVLKVLVTTLSKAFGLNPD
                     EVALLLLSQDRQMLRFAYPPELAKGGTNAFPVSFPSLAGQVAGTGASVLVNNMHEVQH
                     LAIYERIPIMDTEPANIQKLLAVAVEGPDGLPQGVIEVSRRGESLEEVGPDFQAEDQE
                     LLERLAATAAPAIARAFGT"
     gene            complement(2289..2537)
                     /locus_tag="AC16G0_12230"
     CDS             complement(2289..2537)
                     /locus_tag="AC16G0_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100414.1"
                     /translation="MKRPRVFGLGMGVALSVLSLSGLLDLAEARTLHLGFDLRGTQRP
                     ALPILLVTAEAVGPLAVEGVIQRERHPGTASEEDPSHA"
     gene            complement(2534..3268)
                     /locus_tag="AC16G0_12235"
     CDS             complement(2534..3268)
                     /locus_tag="AC16G0_12235"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR5100415.1"
                     /translation="MSPDAARLTMHEFTEAVSQGAWLLLPVGTTEEHGPHLPLGSDML
                     QAEFVASSVASAVDGVVAPGLAYGVCRTTRKFPGTVSLSFHTFATLVREVLEGYVKTG
                     ARRIAIISGHAGGAHLEALHQAAEPLVEREEDLIILILGPADIPLPSLEHAGVSGGDG
                     HAGAVETSVMLAIQERLVHLDRIPAATRPVFPRGQLLRHPESLFPSGIMGEVSPSSRD
                     LGKRVLMAGVAEVARILALASREGAR"
     gene            complement(3265..3552)
                     /locus_tag="AC16G0_12240"
     CDS             complement(3265..3552)
                     /locus_tag="AC16G0_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100416.1"
                     /translation="MSKLCAKCGKEMTDGGLAYEVRIQVYADFDGVLPQVETAEQMEA
                     RLAELVAAMDERDPDELMRDVYHEERHLLCPACRERYLANPLNLPLQEGRL"
     gene            complement(3641..>4038)
                     /locus_tag="AC16G0_12245"
     CDS             complement(3641..>4038)
                     /locus_tag="AC16G0_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HIR5100417.1"
                     /translation="HFALRGELLPRTLNELYERLRDFFVGDDGGEVVGICALSIYWED
                     LAEVRSLAVLETQGGKGLGTALVQACVEEAKRLGVRRVFALTYRPGFFEKLGFQTIDK
                     RQLPQKIWKDCFKCAKVDCCDEVALIRET"
BASE COUNT          752 a         1377 c         1248 g          661 t
ORIGIN      
        1 ggccgccgtc aggggcagga gtccctgcag cgacagcgtg atggccagac cgatggtcgc
       61 cgccgagcta tggaccagtg ccgagaagat ggccgccacg atcagcccca ggagtggctg
      121 gtcacccagg gaaaccagga gcgtcttgac cagctcgctc tggcggagcg gcaccatggc
      181 gtccgacatg agcttcatgg cgaagaagat caagccgaac cccaggatgg cctggccgac
      241 gtatcggaag atcttccgcc tgcatgcgaa gatcaggagg aaacccacgc cgaccagcag
      301 cagggcatag tcgaagatct ccagggcaat gagctggacg gtgaccgtgg ttccgatgtc
      361 ggcgcccagg atcaccccga tggtctgggg gaggcccatg agcccggagc tggcgaagcc
      421 caccagcatt accgtcgttg cgctggagga ctggatgacg gccgtgataa aggccccgac
      481 catcatcccc ttgaagcggt tgttggtgat ggagctgagg atctgccgca tcttgccccc
      541 ggcggcttgc tgcaggccct cccccacgag ctgcatcccg tagaggagga gcgccatgcc
      601 cccgaagagc gtgatgagca tcaggttcgt catcgctctg gcgtcctcac cattccagcc
      661 ttagccggaa ccatccctcg cgctcccctg cccatctggg tcaggatgcg gacggtcgcc
      721 ggaaagacag aaggccagat tgatcattca atctggcctc tccatatctc ggtgtccatc
      781 gaggctagct tgtaacacgc tactgacagg ggtgtcaagg ggaacaggcg aacagccttc
      841 agcctgccgc ccctctgacc aggcgggcga tcaccggagg gatcagtgcc gtggccagat
      901 cggttcggga gccgccgccc aagactacca gcagacgctc agcgcacgac gcatgtgcca
      961 gggcaaccat ccggtctgcg attccccgga aggccgcgct gctcaagccg atatcgccgt
     1021 agtctcctcg gtgcgtgtcg aaaccgaaat accagaccag gaggtccggg tggaaaccca
     1081 cgactcggga gcatgcctcc cgctccagga gcgcaaggta tgcttcgtcg acgacctcgc
     1141 caggtctcag ctcggggaat ggcacatcca cctttgtccc atccgatgac tcgaaggccg
     1201 tgccacagac gcacacatga aggatgtcta gatcggcccg caccagatcg cgtgtcccat
     1261 cagcgtgatg ggcgtcggta tccacgatgg cgattcgtcg gagggatgtc gtccgccgtg
     1321 tttccaccac cgcgatcacg acgtcattga agcagcaata ccccccgaag aagtcccgac
     1381 ccgcatgatg gccgccggcc ccgatgaagc agaaggcgtt gcggagggaa ccggccgcga
     1441 cctgctccat gcctgccacc acgcctccca cggaggcgcg cgccgtggat cagaacgggt
     1501 cggcctcgac tttggggacc agggcagaag cgtggacgcg aaggatctgc tcgtttgtgg
     1561 cggcaggaca ctcttccagg cgaaccaagg ggtgctggcg cagtgcaccc agagcggcgg
     1621 ggaagtctgc gagacgccgg ccggcggtca gatagctccg acggctgaag gagggatgat
     1681 agaagatccc cgtgatggcg ctgatggcgg cagcctgcac gtgcgttccc gccttccctg
     1741 cgggctgcaa aaacgcttac gttccgaagg cccgcgcgat tgcgggcgcc gccgtggccg
     1801 ccagccgctc cagcagttcc tgatcctcgg cctgaaagtc gggaccgacc tcttcgaggc
     1861 tctccccgcg acggctcacc tcgatcacgc cctgcggcaa tccatccggc ccttccacgg
     1921 cgacggccag aagcttctga atgttggccg gctcggtgtc catgatgggg atccgctcat
     1981 agatggccag gtgctgcact tcgtgcatgt tattgaccaa gacgctcgcg cccgtccccg
     2041 caacctgacc ggccaggctg gggaagctca cgggaaaggc attggtcccg cccttggcca
     2101 gctccggcgg atacgcgaag cggagcatct gtcgatcctg gctgaggagc aggagggcga
     2161 cctcgtccgg gtttaatcca aacgccttcg agagcgtcgt gaccagcacc ttgagcacct
     2221 ccgcctgttg ctcggagaac tgtttcccct cgacagtctg gagctgcaag gccaattcct
     2281 gctcccagtc atgcatgact cggatcctcc tcggacgccg tccctggatg ccgctccctc
     2341 tggatcactc cttccacggc caacggcccc acggcctccg cggtcacgag gaggatgggc
     2401 aaggcagggc gctgcgttcc gcgcaggtcg aagccaaggt gcaacgtccg agcttccgct
     2461 aggtccaaga ggccggatag ggagaggacc gagagggcga cccccatccc caggccgaac
     2521 acccgcggac gcttcaacgg gcaccttccc gtgaggccag agccaggatt cgtgcgacct
     2581 cggccacccc cgccatcaaa acccgcttcc ccaaatcccg ggaactggga gagacctcgc
     2641 ccatgattcc ggagggaaac aggctctcgg gatgccgcaa gagctgaccg cgcgggaaga
     2701 caggtcgcgt ggcagcggga atccgatcca ggtgaaccaa ccgctcctgg atcgccagca
     2761 tcaccgaggt ctccaccgca cccgcatggc cgtcgccacc ggagactcct gcatgctcca
     2821 gggacgggag ggggatatcg gccgggccga gaatcaggat gatgaggtcc tcctctcgct
     2881 ccaccagggg ttccgcggcc tggtgcagcg cttccaggtg ggcgcctccc gcgtggccgg
     2941 agatgatcgc gatccgccgg gcccccgttt tgacgtagcc ctccaacacc tcgcggacga
     3001 gggtcgcaaa ggtgtggaag ctgagcgaga cggtcccggg aaacttacgc gtcgtccggc
     3061 aaacgccata cgcgaggccg ggggccacca ctccgtccac ggcactcgcc acgctgctcg
     3121 cgacgaactc cgcttggagc atgtcggagc ccagtggcag gtgtggcccg tgctcctccg
     3181 tcgtgccaac agggagcaga agccatgccc cctgggacac agcctcggtg aactcatgca
     3241 tcgtcagccg tgccgcgtca gggctcacag gcgcccctcc tggagcggga gattcagcgg
     3301 gttcgccagg tagcgctcac gacacgccgg acagagcagg tgccgctcct catgatacac
     3361 atcgcgcatc agctcgtccg gatccctctc gtccattgcc gccaccagct ctgccaaccg
     3421 ggcttccatt tgctcggcgg tctccacctg cggcaggacg ccgtcgaagt cggcatagac
     3481 ctggatgcgg acctcgtagg cgaggccgcc gtccgtcatt tccttcccgc acttcgcaca
     3541 gagcttgctc atgactgttc gatagcgaat cggcctgacc gcccctgcgc ttctcgccct
     3601 ccgcccctcg cttcccgtct tagggtttgc gtctttcgga tcacgtttcg cggatgagcg
     3661 ccacctcgtc acagcagtcg accttcgcgc atttgaagca gtccttccaa atcttctggg
     3721 gaagctggcg tttgtcgatc gtctggaaac ccagcttctc gaagaacccc ggccggtagg
     3781 tgagggcaaa gacccggcgc acgccgaggc gcttcgcctc ctccacgcac gcctggacaa
     3841 gcgccgtccc caaccccttc cccccctgcg tctccagcac cgccagcgag cggacctctg
     3901 ccaggtcctc ccagtagatt gagagggcgc agatgcccac gacctccccg ccgtcatcgc
     3961 ccacgaagaa atcccggagg cgttcgtaca gttcgttcag cgtccggggc agcagctcac
     4021 cgcgcaaggc gaagtgag
//
LOCUS       DBNGZC010000710         4066 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1529043, whole genome shotgun sequence.
ACCESSION   DBNGZC010000710 DBNGZC010000000
VERSION     DBNGZC010000710.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1529043"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="AC16G0_12250"
     CDS             <1..742
                     /locus_tag="AC16G0_12250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR5100418.1"
                     /translation="DPLAVLPWLAGVTQRIAIGTSVVILPYRSPIPVAKLLASVDVLS
                     GGRLIVGAAVGWMEGEFAALGIPIKERASRADEALQLFRSLWTQEYPEVATKRYRLEG
                     VKASPMPLQKPHPPLLIGGGSGGAFRRVARFGDGWHASATTPQAFRQGQEEVNRHWKE
                     LGREGTPIWTLRIPVLVQGVHRPAVDMALIRGRFVIQGPVTKVIEELEEFRNLGCSHV
                     ALEVSYTTFPAILETIDILAQVRPQLAD"
     gene            complement(827..1297)
                     /gene="sixA"
                     /locus_tag="AC16G0_12255"
     CDS             complement(827..1297)
                     /gene="sixA"
                     /locus_tag="AC16G0_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014288849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HIR5100419.1"
                     /translation="MYAFLVQHGEARSEMEDPARPLSQRGREEVERVARHAASLPLRL
                     AEIRHSDKLRARQTAEIFARYLSPPRGIREMDGMAPQDAPGKAREIIQTAAEPLMLVG
                     HLPHLRRLASSLLVGDADKEIVRFRNGAIVCLCRVEEGWVLQWILTPELALRDG"
     gene            complement(1467..1994)
                     /locus_tag="AC16G0_12260"
     CDS             complement(1467..1994)
                     /locus_tag="AC16G0_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018780520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YfiT family bacillithiol transferase"
                     /protein_id="HIR5100420.1"
                     /translation="MADLQYPIGRFVEDREVTDDKRRRWIEEIAAAAAMFRQAVAGLT
                     PEQLDTPYRPGGWTARQVIHHMADSHMHVCMRFRLALTEQEPAITAYNNAAWAELPDA
                     RTGPVESSLALLGALHDRWVLLLRSMTPADFAKTFRRPDGAFVTLDRALQSYAWHGKH
                     HAAHITSLRERMGWN"
     gene            2369..3856
                     /locus_tag="AC16G0_12265"
     CDS             2369..3856
                     /locus_tag="AC16G0_12265"
                     /inference="COORDINATES: protein motif:HMM:NF014073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR5100421.1"
                     /translation="MMQNLVHGFVLTAQWQNIVAIIAGSITGYFVGALPGLSAGMGIA
                     LLLPFTFYMPPLTSLVLLTSLYGAAEYGGSITAVLINVPGEAGATPTCFDGYPLTKKG
                     APGKALGVSIVASAYAGILSTVALILVSIPLAQLALKFGPPEYFALGIFGLTTVAGLA
                     GKSWIKGFITVLFGLLITTIGVDAVSGTSRYVFAKTLYEGIPLIPVLIGLFAVSEVFE
                     TMEELAEKVVYQKFSGSLPTMREYMSTHLAMLRGTIIGFIIGIVPGAGKAVASFIAYN
                     EERRASKHPELFGTGVLEGVAAPEAANNAVVGGALVPLLSLGIPGSAAAAVLIGAFTI
                     QGLQPGPLLFVREPGLVYGLFASLLVGNIVMLAMGLVGTKLWAKVLDVPKNVLTPIVL
                     ATILFAAYAESTNVFSMWLSLFFGILGYLMRKFEFPVAPVVLAMVLGGMIEVSFRRSL
                     ILSDGNPAIFVTRPVAAAILLLAALSIGYQVLRDLRKMRGGTPRR"
BASE COUNT          655 a         1312 c         1335 g          764 t
ORIGIN      
        1 ggatccgctg gcggtcttgc cctggctcgc cggggtcacg cagcggatcg ccatcggcac
       61 gagcgtggtc attctcccgt atcgctcgcc tatccccgtc gccaaactcc tggccagcgt
      121 ggacgtcctc tccggcggcc gcctcatcgt cggcgcggcg gtgggttgga tggaagggga
      181 attcgccgca ctcggcatcc cgatcaagga gcgcgccagt cgggccgacg aggccctgca
      241 gctcttccga tcgctctgga cgcaggagta cccggaggtc gcgacgaagc gctaccggct
      301 cgaaggagtc aaggcctcgc cgatgccgct ccagaagccg cacccgccgc tcctgatcgg
      361 cgggggcagt gggggcgcat tccggcgagt ggcccggttc ggggacgggt ggcacgcctc
      421 cgccacaacc cctcaggcgt tccgccaggg gcaggaggag gtcaatcgcc actggaagga
      481 gctggggcgc gaagggaccc caatctggac gctccggatc cccgtcctcg tccagggcgt
      541 ccatcgtccg gccgtggaca tggcgctgat ccgcgggcga ttcgtgattc agggtccggt
      601 gaccaaggtg atcgaggagc tcgaagaatt ccggaacctt gggtgcagtc acgtggccct
      661 ggaggtgtcg tacacgacgt tcccggccat tctggaaacc atcgatatcc tcgcccaggt
      721 gcggccacag ctggcggatt gacccaaccg agggtccggt cggccgtgcg ggccgccgat
      781 cgatccctga cggtcaaaga ccgccatgga cgcgtgggcg gaaagctcac ccgtccctga
      841 gggccaattc gggcgtcaag atccactgaa gcacccagcc ctcctccacc ctgcagagac
      901 agacgatcgc cccgttccgg aaccggacga tctctttgtc ggcatccccg acgagcaggg
      961 aggaggcgag cctgcggagg tgcggcaggt gtccgacgag catcagtggc tcggcggcgg
     1021 tctggatgat ctccctggcc ttgcccggag cgtcctgggg agccatgccg tccatttccc
     1081 ggatgcctcg cggcggggaa aggtatcggg caaagatctc cgccgtctgt cgcgcgcgga
     1141 gcttgtcgga atggcggatc tccgccaggc gaaggggcag ggatgcggcg tgccgggcca
     1201 cccgctcgac ctcctcgcgc cctcgctgac tcagcggccg tgcggggtcc tccatctccg
     1261 atcgcgcctc accgtgctga accaaaaacg cgtacaccgt caatcctcct cccgatgtag
     1321 acattgacca tcgccggatg taatccggat ttcaccggcg caccatgcta ccctgggtca
     1381 tcctggtgtc caacacgagg cgcaagcccg gtgaccgtga gcaggggaag gtggctgacg
     1441 cccggctgga tctttagcga ggcgttctaa ttccagccca tccgctcgcg cagggaggtg
     1501 atgtgcgcgg cgtgatgctt gccgtgccag gcgtacgact gcagcgcccg gtcgagggtc
     1561 acgaaggcgc cgtcgggacg gcggaaggtc ttggcgaaat ccgcaggggt catggaccgg
     1621 agcaagagga cccaccggtc gtggagcgcg ccgaggagcg ccagggagga ctccaccgga
     1681 ccggtccggg cgtcgggtag ctcggcccag gcggcattgt tgtacgctgt gatggcgggc
     1741 tcctgctcgg tgagggcgag ccggaaacgc atgcagacat gcatgtggct gtccgccatg
     1801 tgatggatca cctgccgggc ggtccagccg ccgggacggt acggcgtgtc aagctgttcc
     1861 ggcgtgagtc cggccaccgc ctggcggaac atcgccgccg ccgccgcgat ctcctcgatc
     1921 cacctccggc gcttgtcatc cgtgacctcc ctatcctcga cgaatcgccc gatggggtat
     1981 tgcagatcgg ccatgggttt cctcctgaca gtgataaggc aagcagaacg atggagcatc
     2041 ctacctccga cagacctccg ttttcaaccg gatgcctggg tttcggcgta acgatcgccc
     2101 tgccgagcat actttcgcct tgacaaggac cgatgctacc tagtaagagc acgcagggcc
     2161 atcggcatga agtctccacg gattgacgca gtggtgcagc tcgcataatt cttcgtttcc
     2221 agttgtgcca tgcgctcgat cctcctgact ccccccgtga gattgccggc ttcaagcagc
     2281 ctcttctcgg cccgaatgct gcgcgcgatg tcggcccagg cttcgaggta cgcccgagga
     2341 agccgtcgcc gccgaggaac cctgttcgat gatgcagaat ttggtccacg ggtttgtact
     2401 gaccgcgcag tggcagaaca tcgtggcgat catcgccgga tccatcacgg gctactttgt
     2461 cggcgcgctc ccagggctca gcgcggggat ggggattgcg ctactcctgc ccttcacctt
     2521 ctacatgccg cccctgacca gcctggtgct cttgacgtcc ctgtacggcg ccgcagaata
     2581 cggcggctcg atcacggcgg tcctgatcaa tgtgcccggt gaagccgggg ccaccccgac
     2641 gtgcttcgac ggctacccac tgaccaagaa gggcgccccg ggtaaggccc tgggggtctc
     2701 catcgtggcc tccgcttacg cgggaatcct gagcaccgtg gcattgatcc tcgtctccat
     2761 tccgctggcc cagctcgctc tgaagttcgg gccgccggag tacttcgccc tggggatctt
     2821 cggactgacg acggtggcag gcctggccgg caaatcctgg atcaagggct tcatcactgt
     2881 cctgttcggg cttctcatca cgaccatcgg tgtggatgct gtgagcggca ccagccggta
     2941 cgtcttcgcc aagaccctgt acgaagggat ccccctgatc ccggttctca tcgggctgtt
     3001 tgccgtctcc gaggtcttcg agaccatgga ggagctggcg gagaaggtcg tctatcagaa
     3061 gttctccggc agtctcccga ccatgcggga gtacatgagc acccatctcg ccatgctccg
     3121 ggggacgatc atcggattca tcatcggcat cgttccgggg gcggggaagg ccgtggcctc
     3181 gttcatcgcc tacaacgaag agcggcgagc ctcgaagcac ccggagttgt tcgggacggg
     3241 cgtgctggag ggagtcgctg cgccggaggc tgcaaacaat gccgtggtcg gcggcgcact
     3301 ggtccccctc ctctccctcg ggattccggg gtcggcggcg gcggcggtcc tgatcggggc
     3361 gttcaccatc caagggctgc agccaggccc gctcctcttc gtgagggagc cggggctggt
     3421 ctacgggctc tttgccagcc tgctggtggg caatatcgtg atgcttgcca tggggctggt
     3481 gggcacgaag ctctgggcga aggtgctgga tgttcccaag aacgttctga cccccatcgt
     3541 gcttgccacc atcctgtttg ccgcctatgc ggaatcgacc aatgtcttca gcatgtggct
     3601 ctctctcttc ttcggtatcc ttgggtacct catgcggaag tttgagtttc ccgtggcccc
     3661 ggtcgtcctg gccatggttc tggggggcat gatcgaggtg tcgttccgtc gatcgctgat
     3721 cctgtcggac gggaatcccg cgatcttcgt cacgaggcca gtggcggcgg cgatcctgct
     3781 tctggccgcc ctgtcgatcg gctaccaggt gttgagggat ttgcggaaga tgcgcggggg
     3841 taccccgaga cgctgaggcg ggtccgcctc ggaaagtgtg ccggaagctg ggcgtccata
     3901 tgacaagccc gtcacaaagg agcgcatggc catgagacta gacagagtgc cgcggatcct
     3961 gtggctgttc ctgggagttg gtctcatcat cctgagccta ctcggatcct tccccagggt
     4021 ctccgacgcc gcgggagcct atccaaccaa gccggtcacc gtcttc
//
LOCUS       DBNGZC010000711         2877 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_745396, whole genome shotgun sequence.
ACCESSION   DBNGZC010000711 DBNGZC010000000
VERSION     DBNGZC010000711.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_745396"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="AC16G0_12270"
     CDS             complement(<1..750)
                     /locus_tag="AC16G0_12270"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5100422.1"
                     /translation="MGTVGIGFIGSRFAADLHAHALGKLRGSKCEIVAVCSRTQESAE
                     AFAKTFKIPHVYTDHRAMLERRDIQLVTLPVVTSLHHSLAIDAANAGKHLIMEKPLTG
                     CFVEAGAKSRQAMFDEAMKNADAVVEACRRNKVRMGYAENFVYAPPVAKLRRLMDASG
                     GTLLDLRAEESHSGSHAAYSRRWATAGGGSMLRMGSHPIGAVIHLKHYEGMKKFGKPI
                     RVKSVMGDTAQLTKVPAFQAEAKKWLVTAWED"
     gene            complement(771..2294)
                     /locus_tag="AC16G0_12275"
     CDS             complement(771..2294)
                     /locus_tag="AC16G0_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR5100423.1"
                     /translation="MVIGPWQILIELWKLFTPLTLAYVTASTFIGIVIGMLPGLTATM
                     GVALLTGLTFGFPPKDAIIILICVYVGAIYGGSRSAILLNIPGTPANAATCLDGFPMA
                     QRGEAGPAIGLATTSSFLGTVFGLFCLAFFTPLLGRIALQFQSWEFFWLAIFGIVVCG
                     NLTAPTDPLKGWIAGFIGLLVSQVGQEGIAAYPRFSFGFDQMRGGFALIPVLVGVYGI
                     AEVLGVMRETAVPRVMTQIKRILPRWRDLWQYRRTILRSGIIGVIIGAIPGVGEDVAA
                     WVSYDFAKRGSQKPEEFGKGSREGLIAAETGNNACIGGAVIPVLSLAVPGSAPAAVLL
                     GAMWLHGVRPGPLLMVEFPNYIWEVTAMFIMASLAMLILGLSLVRTLVKILMVPRPIL
                     MPIVFVLCVIGSFAISVRIFDIWVMLAFGLLGYPMRRYNYPAAPLVLGVILGDMADVN
                     FRRALIRTAGDPTPFFTRPISLILVTITVITILARSAWFRQALATGSARVKAVFSRA"
     gene            complement(2302..2799)
                     /locus_tag="AC16G0_12280"
     CDS             complement(2302..2799)
                     /locus_tag="AC16G0_12280"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR5100424.1"
                     /translation="MRVRRTMAYEFRRQDFYTGLILCCLSIGVIVQCWQMPRDLQGWP
                     AYASPGVVTGLLGLGLLGMGLALTLRSVWRVGAPLGITGADARIYLRDVGTRRLGLMF
                     GLSVIYCLLLGRDLPYWLTTGGYLMAVMFVFRAGPWWQILLISAASTVAVAYVFNRIF
                     AIPLP"
BASE COUNT          574 a          912 c          937 g          454 t
ORIGIN      
        1 atcctcccac gccgtcacca gccacttttt cgcctcggcc tgaaatgccg ggaccttggt
       61 gagctgggcc gtatcgccca tcacggactt cacccggatc ggcttgccga acttcttcat
      121 tccctcgtag tgcttcaggt gaatgaccgc cccgatggga tgggagccca tgcgcagcat
      181 ggagccgccg ccggccgtgg cccagcgccg cgagtaggcc gcatgggagc cggagtggct
      241 ctcctccgcc cggaggtcga ggagggtccc cccgctggca tccatcagcc gacgcagctt
      301 ggccaccggc ggcgcataca cgaagttctc cgcgtatccc atgcgaacct tgtttcgccg
      361 gcaggcctcg accacggcgt cggcattctt catggcctcg tcgaacatgg cctgccgcga
      421 cttggcgcca gcctcgacga agcagccggt cagcggcttc tccatgatga ggtgcttgcc
      481 cgcattggcg gcgtcgatgg ccaggctgtg gtggaggctg gtcaccacgg gcaaggtcac
      541 gagttggatg tccctccgct ccaacatcgc ccgatggtcg gtgtacacgt gggggatctt
      601 gaacgtcttc gcaaaggctt cggcactctc ctgggttctg gagcatacgg caacaatttc
      661 gcacttgctg ccccggagct tgccgagggc gtgggcgtgc agatccgcgg cgaaccgcga
      721 gccgatgaag ccgataccga cggtgcccat gggctccact ccttgccgga ctaggcgcgg
      781 gagaagacgg ccttcacccg tgccgatccg gtcgcgagcg cctgccggaa ccaggcggaa
      841 cgggctagga tggtgattac ggtaatcgtg accaggatca gcgagatggg tcgagtgaaa
      901 aatggcgtcg gatcacccgc agtacgtatc agcgcgcggc ggaagtttac gtcagccata
      961 tcccccagga tgacgccgag gaccagcggt gcggcggggt agttgtacct ccgcatggga
     1021 taacccagga ggccgaaggc cagcatcacc cagatgtcga agatgcgtac actgatggcg
     1081 aaggagccga tgacacacaa gacgaagacg atcggcatca ggatggggcg aggcaccatg
     1141 aggatcttca ccagggtgcg caccagggag agtcccagga tcagcatggc gagggaggcc
     1201 atgatgaaca tggccgtgac ttcccagatg tagttgggga actcgaccat gaggaggggg
     1261 cccggacgca ccccgtgcag ccacatggcg ccgagcagga ccgccgcggg tgcgcttccg
     1321 ggaaccgcca gggacagcac cgggatcacc gcgcccccga tgcaggcatt gtttcccgtt
     1381 tcggccgcga tgagcccctc ccgcgatccc ttgccgaatt cttccggttt ctggctgccc
     1441 ctcttggcga agtcgtagga gacccaggcg gccacgtcct cgcccacacc gggaatggcc
     1501 ccgatgatga ctccgatgat cccggagcgc aagatagtcc ggcgatactg ccagaggtct
     1561 cgccatcggg gcaggattct cttgatctgg gtcatgaccc gtgggaccgc ggtctcacgc
     1621 atgacgccca ggacctcggc gatgccgtaa accccaacca acacggggat caacgcgaac
     1681 ccgccgcgca tctggtcaaa gccgaaggag aacctcgggt aggcggcgat cccttcttga
     1741 cccacctgcg agaccaacag gcctatgaag ccggcgatcc atcccttcag ggggtcggtg
     1801 ggcgcggtga ggtttccgca tacgacgatc ccgaagatgg cgagccagaa gaactcccac
     1861 gactggaact gcagggcgat ccttcccagg agcggggtga agaaggccag gcaaaacaag
     1921 ccgaagacgg tcccgagaaa ggaggaggtc gtggccaggc cgatggccgg gcccgcctcg
     1981 ccccgctggg ccatcgggaa gccgtcgaga caggtcgcgg cattcgccgg ggtcccaggg
     2041 atgttcagca ggatggccga tcggctgccc ccatagatcg ctcccacgta cacgcagatg
     2101 agaatgatga tcgcatcttt cggcgggaag ccgaaggtca ggccggtgag cagggcaacg
     2161 cccattgtag ccgtgaggcc gggaagcatc ccgatgacga tgccgatgaa cgtgctggca
     2221 gtgacgtaag ccagcgtgag gggggtgaat agcttccaca actcgatcag gatttgccag
     2281 ggccctatga ccatcgcagc ctcacgggag cggaatggcg aagatccggt tgaatacata
     2341 ggcgaccgcc acagtggagg ctgccgaaat gagcaggatc tgccaccacg gtccggcgcg
     2401 gaacacgaac atcaccgcca tcaggtatcc cccggtggtg agccaatatg gaagatctct
     2461 tcccaacaaa aggcagtaga tgacgctgag cccgaacatc agcccaagac gacgggtccc
     2521 gacatccctg aggtagatcc gggcatccgc acctgtgatc ccgagtgggg cgccgacccg
     2581 ccacacggag cgcagcgtga gtgccagccc catcccgaga aggcccaggc ccaggagccc
     2641 ggtgaccacc cccgggctcg cgtaggcggg ccagccctgc aggtcgcggg gcatctgcca
     2701 gcactgcacg atcacgccaa tgctgaggca gcataagatg agcccggtgt agaagtcctg
     2761 ccgccggaat tcgtacgcca tggttcgcct tactctcatg aggcaggagg gggccggcct
     2821 tggcctcttg aaggccaggg ccggcccact gctgccgatg actttgcgag ctagcgc
//
LOCUS       DBNGZC010000712         1915 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_358193, whole genome shotgun sequence.
ACCESSION   DBNGZC010000712 DBNGZC010000000
VERSION     DBNGZC010000712.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_358193"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="AC16G0_12285"
     CDS             <1..757
                     /locus_tag="AC16G0_12285"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HIR5100425.1"
                     /translation="YPSAVVDDIAGSSDQESGMEAHASLMELLEGVHDPRRAQGKRHP
                     LPALLGLAVVAMLAGMTSYEAIVQYGKERGWAFLQHLGFTTRRGLCKATYSRVFRRID
                     VADFETRLGQWLRGHLGQDDAPHIAVDGKTARGSRDGTTPGVHLLAAYAPDVKAVLAQ
                     LRVDSKTNEHKAALELLGVLALKGKVVTGDAMFTHRDVCTKVIGSGGNYILPAKENQS
                     TLCRDITAVFAAPEAGLSPPPNYATKGRDRVHS"
     gene            780..1199
                     /locus_tag="AC16G0_12290"
     CDS             780..1199
                     /locus_tag="AC16G0_12290"
                     /inference="COORDINATES: protein motif:HMM:NF033564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100426.1"
                     /translation="METRSIEVTAALAEYLGSDWPGCEQVFRLTRVRKTGAEVETEVV
                     LGITSLPRERAGAKALLGLIRDHWGIENGLHGVRDGTLREDASRIRKGSGPEVMAGLR
                     NIVVFLFERMGYKSAAAATRHYVCHPEETLRIVSTRI"
     gene            1735..>1915
                     /locus_tag="AC16G0_12295"
     CDS             1735..>1915
                     /locus_tag="AC16G0_12295"
                     /inference="COORDINATES: protein motif:HMM:NF016167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HIR5100427.1"
                     /translation="MFDFNVSETSPVVKGTWVTVSHIVSLIVDGWTWSDILRTHPELT
                     EEDIRTCLAYTVAQDE"
BASE COUNT          348 a          622 c          632 g          313 t
ORIGIN      
        1 atatccatcc gctgtggtcg atgacatcgc ggggtcttcg gatcaggagt ctggcatgga
       61 agcccacgcc tctttgatgg aactgcttga gggagttcat gatccacgac gggcccaggg
      121 caagcggcac ccgcttcccg ccttgctcgg cttggccgtc gtcgccatgc tcgcgggcat
      181 gacttcttat gaggcgatcg ttcaatacgg caaggaacgg ggttgggcgt tcctccaaca
      241 cctcgggttc accacccgtc ggggcctctg taaggccacc tattcgcggg tcttccgccg
      301 catcgacgtc gccgacttcg agactcgact gggccagtgg ctccggggac acctcgggca
      361 ggacgacgcc ccgcacatcg ccgtcgacgg caagaccgcc cggggcagtc gcgacggcac
      421 gacgcccggg gtccacctgc tcgccgccta tgccccggac gtcaaggcgg tcttggccca
      481 acttcgggtg gattccaaga ccaacgaaca taaggctgcg ttggaactgc tgggcgtgct
      541 ggctctgaag ggcaaggtcg tcaccggaga tgccatgttc acgcaccgcg acgtctgcac
      601 gaaggtgatc ggatcgggcg ggaattacat cctgccggcg aaggagaatc agtcgaccct
      661 ctgccgggat atcacggccg tattcgccgc gcctgaggcc ggcctttccc cccctccaaa
      721 ctacgcgaca aaaggccgag atcgagtgca ctcgtgaggt ccacaaggga cacggtcgga
      781 tcgagacgcg gtcgattgaa gtcactgccg cgctggccga gtacctgggc tcggactggc
      841 ccggatgcga gcaggtgttt cgtctgacgc gcgtgcgcaa gacgggcgcg gaggtcgaga
      901 ccgaggtggt gctggggatc acgagcttgc cgcgcgagcg ggccggggcc aaggcgctct
      961 tgggactgat ccgcgaccat tggggcatcg agaatggcct gcatggggtc cgtgatggga
     1021 cgctccgcga ggacgccagc cggatccgca agggttccgg gccggaggtg atggcggggt
     1081 tgagaaacat cgtcgtcttc ttgttcgagc gaatggggta caagagcgct gcagcagcga
     1141 cgcggcatta cgtttgtcac ccggaagaga cgctgaggat cgtgtcaacc cgaatctgag
     1201 aatgaaacgg ccctgcgtgg agcgtgaaga caactcgtca ggagctccca attcgtttac
     1261 gatcttcgcc gactgtctcc gcaaggagct cgcccccccg acactcctcg agcaactcgt
     1321 cgccgagcgg ctgatcaacg cggcgtggcg cctgctctgg gcgacgaatc tggaagccga
     1381 agccctggaa aaggccgccg ctgcgagtgg gaaagcctct tcccaacggt ccccacgtac
     1441 cgccacgtcg gcccggcgtg aactgctccg ggcggagcgc ggcctggagc gcgccctcca
     1501 cctgtattac agaacgcgag agagccatca accccgctgg ggcaaggcgg aacacaccgc
     1561 cgcgccgccg gtgaccgccg cagcgtccgc ggtagcgtat tcccccccga acccccgtcc
     1621 ctctcgaagg aacggccccg tgcctcctcc agtgagcggt cgctcgaccc cgaagcggag
     1681 agagaggcac ccgcggagga agccgaggct tgcgggcgct ggcaggaccg cctggtgttc
     1741 gacttcaacg tctcggagac ctcgccggtg gtcaagggaa cctgggtcac tgtcagccac
     1801 atcgtctcgc tgatcgtgga cggctggacc tggtccgata tcctgcgcac ccaccccgag
     1861 ctgacggaag aggacatccg tacctgcctg gcctacaccg tggcccagga cgaag
//
LOCUS       DBNGZC010000713         3341 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2214515, whole genome shotgun sequence.
ACCESSION   DBNGZC010000713 DBNGZC010000000
VERSION     DBNGZC010000713.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2214515"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1658)
                     /locus_tag="AC16G0_12300"
     CDS             complement(<1..1658)
                     /locus_tag="AC16G0_12300"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5100428.1"
                     /translation="MDATARWYRNLSLRTKFALYMVVSIAFLFAVLIPGVVYLQQRTV
                     LDEVRERGFLLTKVFAHSSVQSLVADDFLVMRQIINSVASDPAVLYAMILDPAGNLIM
                     HSDMREVGRKYTDPASRRAAQAEQPLLQVVWRPRRHLYDFSVPIYILNERRAVARIGI
                     SLEREIADIRRTRNLVFAFGIIALAAGLGLAMWQANDIIRPVGELVRGAEEIAAGNLG
                     RNIPVHARDEVGHLAAAFNRMAESLRVRFEVDRELSATLNLEAVLRALTRHAHKLSDA
                     DMAFLAYRESGSPDASVAASSGTVGAAIGTWRIRAGQGRAGCILSGSGTSSLPEPAAP
                     VDPDEERVLREEGVSALLLVPISFRNVCVGVLGVGRRLATTFDEGAAEAIQRLADQAA
                     VALANALAYREIELLNLSLEAKVAERTRELVEANAALKASDQKLRALDRLKSEFVSNV
                     SHELRTPLTAIRMSVDNLLDGVTGEISPMLERYLTRVKGNTERLVRLITDLLDLSRIE
                     AGRIELHRTTVNAGDVIQDVVESLRPMAADKGLELAVTQSDGPLL"
     gene            complement(1724..3166)
                     /locus_tag="AC16G0_12305"
     CDS             complement(1724..3166)
                     /locus_tag="AC16G0_12305"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5100429.1"
                     /translation="MGQPPKTFVCVLEGEPETLDPGAKFYSERANRVKWLLFDALINI
                     AGDGRRLEPGLAEKWRLSDGGLCAELTLREGILFHDGTAVDAEAVKACFQRQFALDLN
                     DPKKQVLRDLIQDIHTPDRHALVFKMRYPGFEYLARRYLYKLGVVSPTALAKLGKDFA
                     RRPVGTGPFKDPVWLEDRIVLSRNPGYWAGPPRVDEVHFRIIPDGHEAVDALLAGGVD
                     FVPSLSDPDSIQRVLQDDRVRIQVVPGYNVYYLGVYWTKSPFDNPTMRRAVAQGVDVH
                     KAMFIGKGAAAAAVGPLPPHMQGYDPCLRQLPHDPDAARTLLREAGYDHHPVILVHYG
                     PASFFRNLALAVERDLTQIGMHVRRREMPTWPEFLAAVKNADGNMFLYSWHMRTEDAH
                     GFLRALFHSSNIGVTNLTGYSNPEVDKALDMAPPHEYSTAQRIILEDIPMVFLAHWTR
                     VAAYRSRVRNLRVNLGVLPQDKLVGVDLSE"
BASE COUNT          633 a         1033 c         1063 g          612 t
ORIGIN      
        1 aggagcggcc catccgactg ggtgaccgca agctccaagc ctttgtcggc ggccatgggg
       61 cggaggctct ccacgacgtc ctggatgacg tcgccggcgt tgactgtcgt ccgatgcaac
      121 tcgatccggc cggcttcgat ccgcgacagg tccaagaggt cggtgatcag acgaaccagc
      181 ctttcggtgt tgcccttcac cctcgtaaga taccgctcca gcatcgggct gatctccccg
      241 gtgacgccat ccaggaggtt gtccacggac atgcggatgg cggtgagcgg ggtgcgcagc
      301 tcgtgggaga cgttggacac aaattcagat ttcagacgat ccagggctcg cagtttttga
      361 tcggaggctt tcaaggccgc attggcttcc acaagctcgc gggtgcgctc agccaccttg
      421 gcctccaggc tcaggttcaa cagctcgatc tcccggtaag caagggcatt ggcgagcgcc
      481 acggcggcct ggtctgccag tcgctggatg gcctccgcag ccccttcgtc gaaggttgtc
      541 gccagccgcc ggcccacgcc cagtaccccc acgcatacat tgcgaaaact gatggggacc
      601 aggagcaagg ccgacactcc ctcttccctc aggacgcgct cctcgtccgg atcaaccggg
      661 gcggcaggtt cgggcagcga ggaagtcccg ctgccggaaa ggatgcatcc ggcgcgacct
      721 tggcccgccc ggatgcgcca ggttcctatg gcagccccca ccgttccgga ggaggccgcc
      781 acgctggcgt cgggggatcc cgactcgcgg taggcgagga atgccatgtc ggcgtcggac
      841 aacttgtggg cgtggcgggt gagagcccgc agcaccgcct ccaagttcag ggtggcggag
      901 agttcgcgat ccacctcgaa acggactcgg agggactcgg ccatccggtt gaatgccgcg
      961 gcgagatgcc ctacctcatc ccgggcgtgc accggaatat tgcgacccaa gtttccggcg
     1021 gcgatctcct ctgcgccgcg gacgagctcg ccgacgggac gaatgatgtc gttggcctgc
     1081 cacatggcca ggcccagacc cgccgcgagg gcaatgatgc caaacgcaaa gaccaggttc
     1141 cgggtccgcc ggatatcggc aatctctcgc tccagggaaa tgccgatgcg cgccacggcg
     1201 cgccgctcgt tgaggatata gattgggacg ctgaaatcat acaggtgtcg gcgcgggcgc
     1261 cacaccacct gcaacagggg ctgctcggcc tgggcggcgc gtcggctcgc cggatcggtg
     1321 tatttccggc ctacctcgcg catgtcgctg tgcatgatga gatttcccgc ggggtcgagg
     1381 atcatcgcat acaggacggc cgggtcgctg gcgacgctgt tgatgatctg ccgcatcacg
     1441 aggaagtcgt cggccacgag ggattggacg ctggaatggg cgaacacctt ggtgagcagg
     1501 aatcctcgct ctcgcacctc gtccaggacg gttcgttgtt gcagatacac cacccctgga
     1561 atcaggacgg cgaagaggaa ggcgatggac acaaccatgt agagggcgaa cttggtgcgg
     1621 aggctcaggt tccgatacca cctcgccgtt gcatccatcc actttcgaaa cgtgaggttc
     1681 acgggaacct ctcggtcacg aggaaacgtt gggtgccgat cgcctactcc gacagatcca
     1741 caccaaccaa cttgtcctgt gggaggacgc caagattcac gcgaaggttc cgcaccctcg
     1801 agcgatacgc cgccacccgg gtccagtgag ccagaaagac catggggata tcctcgagga
     1861 tgatccgctg agcggttgaa tactcgtgag ggggagccat gtcgagggcc ttgtccacct
     1921 ccggattcga gtagccggtg agatttgtga cgccgatatt gcttgagtga aagagcgcgc
     1981 gaagaaaacc gtgcgcgtcc tcggttcgca tatgccagct atacaggaac atgtttccgt
     2041 ccgcattttt cacagccgcc aagaattcgg gccaggtcgg catctccctc cgcctgacat
     2101 gcatgccgat ctgggtgagg tcccgctcga cggcaagggc gagattgcgg aaaaatgagg
     2161 cggggccgta atggacgagg atcaccgggt ggtgatcata ccctgcctcc cgcaggagcg
     2221 tcctggccgc atccggatcg tggggaagct gtctgagaca tggatcgtat ccctgcatgt
     2281 gcggtgggag cgggcccacg gcagccgcgg ctgcaccctt cccgatgaac atggccttgt
     2341 gcacgtcaac accctgagcg acggcccgcc gcatggtcgg attgtcaaag ggactcttgg
     2401 tccaatagac acccaggtaa tagacgttgt acccggggac cacctgaatc cggacccgat
     2461 catcctgcag cacgcgctga atcgagtcgg ggtcggagag gctcggcaca aaatccacgc
     2521 cacccgccaa gagcgcgtcc acagcctcgt gtccatcagg gatgatgcgg aagtgcacct
     2581 catcgactct gggcggtcct gcccaatacc cgggattcct ggataggacg atgcgatcct
     2641 ccaaccacac gggatccttg aaggggccgg tgccgacggg acgtctcgcg aaatccttgc
     2701 cgagtttggc cagagcggtt gggctgacga ccccaagttt gtataggtac ctccgggcca
     2761 gatactcaaa cccggggtat ctcatcttga agaccagggc atgccgatcc ggggtgtgga
     2821 tgtcttggat gaggtcccgg agcacttgct tcttggggtc gttcagatcg agggcaaact
     2881 ggcgctggaa gcacgccttg accgcctccg catccacggc agtcccgtcg tgaaagagga
     2941 taccctcgcg aagagtcaac tctgcgcaga ggccgccgtc ggaaagcctc cacttctcgg
     3001 ccaggcccgg ttccagacgg cggccatcgc ctgcgatgtt gatgagggcg tcgaagagca
     3061 gccacttgac ccggttggcc cgctccgagt agaacttcgc ccccgggtcc agcgtttccg
     3121 gctccccttc cagcacgcag acaaatgtct tgggcggttg acccatgagc ttctcggttc
     3181 tgtgtgggct cccggtcgtc gggtcgagac tttcaccaat cggaccggtg gcgagtgggt
     3241 gccggctcag gcatgcatgt cgacatccac ctcggtcagc ctattcctgg gaagggtccc
     3301 atcaagtatc gtaagatgct tgaccctggt gttgtatgcg g
//
LOCUS       DBNGZC010000714         1997 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1333267, whole genome shotgun sequence.
ACCESSION   DBNGZC010000714 DBNGZC010000000
VERSION     DBNGZC010000714.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1333267"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="AC16G0_12310"
     CDS             <1..570
                     /locus_tag="AC16G0_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS701 family transposase"
                     /protein_id="HIR5100430.1"
                     /translation="IEDVTTYPAELQTVVFPQTTRELRRQVIRDVLWYRGCKDEPVTV
                     VLVRDPAGQWRDEALVATDPTAAAEFVILGYCRRWSVELAFYDSKQFLGLHDPRVRSA
                     RSVERAHPMAWFVGSLAVLWYCLKGHEGSHVIKDRPWYKQRVTPTFTDMLGALRLQMW
                     EHAVFGESGERLPSPECIENLLHKMAAVA"
     gene            665..1879
                     /locus_tag="AC16G0_12315"
     CDS             665..1879
                     /locus_tag="AC16G0_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HIR5100431.1"
                     /translation="MRPEPEMIPALIDAAKALKGSQRRLFMAKTVQAMGRGGQRWAEE
                     YLGWCRDTIRKGTHELESGMTCVDAFSARRRKPAEEHLPGLLDDIRDLVDGQCQADPK
                     FQTQRLFIRISAAEVRSRLITRKGYTDAELPTPQTINTKLNMLGYRLTKVAKCRPRKK
                     VPQTDAIFEQLSQINRQADESPETLRLSLDAKATVHIGPFSRRGRSRTKTEAADHDFK
                     PEATLTPFGILLPEDDELMLYMTGSPVTSDFIVDRLKDWWEFNRERFPEVTKLVLNLD
                     NGPENHSRRTQFLKRMVGFARGYQLDIELAYYPPYHSKYNPIERCWGILELHWNGALL
                     DSVEAVLGYAETMTWKGGHPEVVLVETEYSKGVRLSREEMEALESEVTRLPSLEKWFV
                     SISGRPKKRRAS"
BASE COUNT          387 a          608 c          686 g          316 t
ORIGIN      
        1 atcgaggacg tgacgaccta ccccgccgag ttgcagaccg tcgtcttccc gcagacgacg
       61 cgggaactgc ggcgtcaggt gatccgcgac gtgctctggt accgcgggtg caaggacgaa
      121 ccggtcacgg tggtgctggt ccgcgacccg gcgggccagt ggcgggacga ggcgctggtg
      181 gcgaccgacc cgacggccgc ggcggagttc gtgatcctgg ggtattgccg gaggtggagt
      241 gtggagctgg cgttctacga tagcaaacaa ttcctcggcc tgcacgaccc gcgggtgcgc
      301 agcgcgcgga gcgtggaacg tgcgcacccg atggcgtggt tcgtcgggtc gctggcggtc
      361 ctgtggtact gcctgaaggg tcatgagggc tcgcacgtca tcaaagaccg tccgtggtac
      421 aagcagcggg tcacgccgac gttcacggac atgctgggtg cgctgcggtt gcagatgtgg
      481 gagcacgcgg ttttcggaga gtcaggtgag aggctcccct ctccggaatg catcgaaaac
      541 ctgctccaca agatggccgc agtggcgtag aggtccgaaa gtctagtcag attaggggct
      601 tgaaagaaga aaagtcgccc gtatcctgga gcctctacac caagaggctc aggagcttac
      661 ggagatgcga cccgaacccg agatgatccc cgccctcatt gacgccgcca aggctctcaa
      721 agggagccag cggcggctct tcatggccaa gacggttcag gcgatgggac ggggtgggca
      781 acgctgggcc gaagagtacc tcggatggtg ccgggacacc atccgcaagg ggacgcacga
      841 actcgaatcc ggcatgacct gcgtcgatgc cttctcggcc cgccgccgca agcccgccga
      901 ggaacacctg cccgggctgc tcgatgacat ccgggacctc gtcgatggcc agtgccaggc
      961 cgatccgaag ttccagaccc agcggctctt catccgcatc agcgccgccg aggtccggag
     1021 tcggctgatc accaggaagg gctacaccga cgccgagctg cccaccccgc agacgatcaa
     1081 cacgaagctc aacatgctgg gctaccggct caccaaggtg gccaagtgcc gccctagaaa
     1141 aaaagtccca cagaccgacg ccatcttcga gcagttgagc cagatcaatc gccaggccga
     1201 cgagtccccg gagacgctcc gactctcgct cgacgccaag gcgacagtcc acattggccc
     1261 gttctcccgc cggggcagga gccggaccaa gaccgaggcg gcggaccacg acttcaagcc
     1321 cgaggcgacg ctgacaccct tcggcatcct cctgcctgag gacgacgagc tgatgctcta
     1381 catgaccggc tcaccggtca ccagcgactt catcgtggac cggctgaagg attggtggga
     1441 gttcaaccgg gagcggttcc ccgaggtcac caagctggtg ctgaacctgg acaacgggcc
     1501 ggagaaccac agccgccgga cgcagttcct caagcggatg gtcgggttcg cccgtgggta
     1561 tcagctcgac atcgagttgg cgtattaccc gccgtaccac agcaagtaca acccgatcga
     1621 gcggtgctgg ggcatcctgg agttgcactg gaacggggcg ttgctggatt cggtcgaggc
     1681 ggtgctgggc tacgccgaga cgatgacctg gaagggcggg catcccgaag tcgtgctggt
     1741 cgagacggag tacagcaagg gggtgaggct gagccgggag gagatggagg cgttggagtc
     1801 ggaggtcact cggctcccct cactggagaa gtggttcgtg tccatctcgg ggaggccgaa
     1861 aaagagacgg gcgagttaac ttctttcaaa ccccttaccc tcgatggccc agttcggtta
     1921 ggggttcagg tgcggcgctg tcgggaccgg gactgcgcac gttgcggccg gtcggtgcgg
     1981 cccgaggccg aggggcg
//
LOCUS       DBNGZC010000715         4421 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_648464, whole genome shotgun sequence.
ACCESSION   DBNGZC010000715 DBNGZC010000000
VERSION     DBNGZC010000715.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_648464"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..968
                     /gene="ccmA"
                     /locus_tag="AC16G0_12320"
     CDS             207..968
                     /gene="ccmA"
                     /locus_tag="AC16G0_12320"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HIR5100432.1"
                     /translation="MTEPMVEVEGLTKVFGGTLALDNVDLRVRPGEVAALLGPNGAGK
                     TTLLKLLSTALKPTAGGGRILGEDLLKGRDAIRRRIGMISHNHHLYEDLTADENLQFA
                     AAMHGMRTDDGAIRKALTEVGLGAQARWKVRTFSTGMKRRLVIGKMILFAPDLLFLDE
                     PHNTLDQAAIALLNGYVASVTARGGAAIVATHNLTRAYAMADRFVLMRDGAVAWRVDR
                     QGLLPDQLRELYEQHAESEETGGQATASGAEASRW"
     gene            962..1645
                     /locus_tag="AC16G0_12325"
     CDS             962..1645
                     /locus_tag="AC16G0_12325"
                     /inference="COORDINATES: protein motif:HMM:NF015344.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA];
                     GO_process: GO:1903607 - cytochrome c biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1904334 - heme import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HIR5100433.1"
                     /translation="MVAGVLRRTWAMTWKDVTVELRARERVNAMLFFAALVLFIFNFA
                     LGPDREKLREVAPGLLWLAFLFTGMLGLGRSFQAERENECFEELLLTPGDRESIYFGK
                     LVGNILFMVTAEAFILPLFGILYNLDIWDKLIPLALVAFLGTVGFSAIGTLLAAMTAH
                     LRAREVMLPLLLFPLTVPVILASVRSTEAILNGGGLSEVGNWLKLLGGFDLIFAVVCP
                     LAFEFVLEE"
     gene            1645..2337
                     /gene="ccsA"
                     /locus_tag="AC16G0_12330"
     CDS             1645..2337
                     /gene="ccsA"
                     /locus_tag="AC16G0_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HIR5100434.1"
                     /translation="MLSSNVAAKGVGAGAGLLLLAGLYLGLVAAPPDAVQGNVQRIMY
                     LHVPSIVTAYVAFFVVFCTSILYLWKRDLRYDAVAVASAEIGVVLTGITLATGSIWGK
                     STWGVWWTWDARLTLTAILFTIYVGYLMLRSFSEDPRAAARYGAVLAIIGFLDIPMNH
                     FAVYWWRTLHQPSSILRAGGPSVAPVMLVPLAVNFVGLVLLYTYFLLERFRLERIRHK
                     TAEVRLKRGRRG"
     gene            2399..2545
                     /gene="ccmD"
                     /locus_tag="AC16G0_12335"
     CDS             2399..2545
                     /gene="ccmD"
                     /locus_tag="AC16G0_12335"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF016856.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HIR5100435.1"
                     /translation="MEHWGYVWLAYGVAAVALCGYLVLLKGRLRDAGTELSVLEQDSG
                     RRTR"
     gene            2542..2988
                     /gene="ccmE"
                     /locus_tag="AC16G0_12340"
     CDS             2542..2988
                     /gene="ccmE"
                     /locus_tag="AC16G0_12340"
                     /inference="COORDINATES: protein motif:HMM:NF009727.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HIR5100436.1"
                     /translation="MKGPKLKYLVGGAIVIAALLGLVYSGVKESAVYFYTPSELNQQK
                     DSVAGKSLRVGGMVMDGSVKWDPQSLLLTFKLTDGQGTVLVRHKGTAPDLFKEGAGAV
                     VEGTWSPDGYFRSSTIMAKHSEEYKPPTHPETLESKKEMFKSILKK"
     gene            2997..>4421
                     /locus_tag="AC16G0_12345"
     CDS             2997..>4421
                     /locus_tag="AC16G0_12345"
                     /inference="COORDINATES: protein motif:HMM:NF013725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HIR5100437.1"
                     /translation="MVSTLGGFVLFLALGVTIYAAAASVIGARPNREDWLASSRNAIL
                     VHFCLVTLAIFLLEYALITSDFSLRYVAFNSTRGSLTRYKIAGLWGSLEGSILLWEWL
                     QALMSALVVARYHDKHRQLMPYVQAVLQGISIFFLLLMTFAVNPFARIFPIPPDGRGL
                     NPLLEVTDMLVHPLLLYSGYVGFSVPYAFGMAALITGRPSEEWLSITRRWTVVAWMFL
                     TCGIFYGGWWSYRTLGWGGYWAWDPVENASFMPWLTGTAFIHSVMIQQRKRMLRVWNL
                     ALVTLTFSLVIFGTFLTRSGILGSVHAFADGPVGILFLAFLSLVLLFSFGLVAYRSDQ
                     LKGHGELDSIVSRESAFLLNNVVLVGICFTVFLGTIFPLLSEAIRGTKISVGTPYFNR
                     VAGPLGMALLLLMGVGPLIAWGRASLNNLRRNFLKPSLLAIVGTIIVYILGVHEDAAL
                     AAFLFAFFVIATVSFEFVISTRTRA"
BASE COUNT          670 a         1346 c         1538 g          867 t
ORIGIN      
        1 gcttgcgtct cccggtgttc ccggcgagag gccgctggca cgatgatccg gtccggcaag
       61 ggtccacgcg tttgcgagtg ccgcgggatg cggcgagcca gcccagaaca gaggcgggga
      121 ggcgtgaggc ggggccggga ccgtaccgga cgaacgggga tgcgaagcgg tcagggcaga
      181 aatccccgaa cggggagagg aatggcgtga cggaacccat ggtagaggtc gagggcctca
      241 cgaaggtctt cgggggaacc ctcgccctgg ataacgtgga tctcagggtg aggcccggcg
      301 aggtcgcggc gctgctcgga cccaacgggg cggggaagac cacgctcctc aaactcctgt
      361 ccacggcgct caagcccacg gcgggcggcg gccgcatctt gggcgaggat ctcctcaagg
      421 ggcgggacgc catccgtcgc cggatcggga tgatctccca caaccaccac ctctacgaag
      481 acttgacggc ggatgagaac ctccagttcg ccgcggccat gcacgggatg cgcaccgatg
      541 acggggcgat ccggaaggcc ctgaccgagg tcgggctggg ggctcaggcc cgatggaaag
      601 tccggacctt cagcacgggt atgaagcggc gactcgtcat cggcaagatg atcctcttcg
      661 cgccggacct cctgttcctg gacgagcctc acaacaccct ggaccaggcg gccattgccc
      721 tgctgaacgg ctacgtggcg tcggtgacgg cccggggcgg ggccgccatc gtggccaccc
      781 acaatttgac gcgcgcctac gccatggccg accggttcgt gctcatgcgg gacggggcgg
      841 tggcctggcg tgtggatcga cagggactac tccccgacca gctccgcgag ctgtacgagc
      901 agcacgccga gagcgaggaa accggtgggc aggctacggc ctcgggggcg gaggcgtcgc
      961 gatggtagcg ggggtgctgc gccgcacgtg ggccatgacc tggaaggacg tcaccgtgga
     1021 gctgcgcgcc cgcgagcgcg tcaacgccat gctattcttc gcggccctgg tgctcttcat
     1081 cttcaacttc gccctgggac ccgatcggga gaagctgcgg gaagtcgcgc cgggcctcct
     1141 ctggctggcc ttcctgttca ccgggatgct gggtctgggg cgcagctttc aggcggagcg
     1201 ggagaacgag tgcttcgaag agctgctcct cacccccggg gatcgagagt ccatctactt
     1261 cggcaagctg gtggggaaca tcctcttcat ggtcacggcg gaggccttca tcctcccgct
     1321 tttcggcatc ctctataacc ttgacatctg ggacaaactc atccccctgg ccctggtggc
     1381 cttcttaggg acggtgggat tctcggccat cggcacgctc ttggcggcaa tgacggcgca
     1441 cctgcgtgct cgcgaggtga tgcttccgct gctgctgttt cccctcacgg tgcccgtgat
     1501 cctcgcctcc gtccggtcga cggaggcgat cctgaatggc ggcggcctgt cagaggtggg
     1561 caactggctc aagctgctgg gggggttcga tctgatcttc gcggtggtgt gcccgctggc
     1621 attcgaattc gtgctggagg aatgatgctg agcagcaatg tggcggccaa gggtgtgggc
     1681 gcgggggccg ggttgcttct gctggcgggt ctgtacctgg gattggtggc agccccgccg
     1741 gacgcagtgc agggtaacgt gcagcgaatc atgtacctgc acgttccctc catcgtcacg
     1801 gcatacgtgg cgttcttcgt ggtgttttgc accagcatcc tgtacctgtg gaagcgggat
     1861 ctgcgctacg atgcggtggc ggtggcctcg gccgagatcg gggtggtcct caccgggatc
     1921 acgctggcca cggggtccat ctggggtaag tcaacctggg gtgtgtggtg gacctgggat
     1981 gcccgactca ccctgaccgc catcttgttt acgatctacg tggggtacct gatgctgcgc
     2041 tccttctccg aggacccgcg ggcggcggcg cgctacggcg cggtcctggc catcatcggc
     2101 ttcctggaca tccccatgaa ccactttgcg gtgtactggt ggcgtacgct ccaccagccc
     2161 tcctcgatcc tgcgggcggg cggcccatcg gtggccccgg tgatgctggt gcccctggca
     2221 gtcaacttcg tggggctggt cctgctgtac acctatttcc tgctggagcg gttcaggttg
     2281 gagcgcattc gacacaagac ggccgaggtc cggctgaagc gagggcgccg ggggtaaggg
     2341 cgcaaaccgg agggtccgtc gcagggggaa gaggcatttc catttcgcgg gaggtagcgt
     2401 ggaacactgg ggttatgtgt ggttggcgta cggggtggcg gcggtggcgc tctgcgggta
     2461 cctcgtcctc ctgaagggcc gcctgcggga tgccgggacg gagctgtccg tcctggagca
     2521 ggacagcggt cggaggacac gatgaaggga ccaaagctga agtacctcgt gggaggcgcc
     2581 attgtgatcg cggcgctcct cgggctggtg tattccggcg tgaaggagtc ggcggtgtac
     2641 ttctacacgc cgagcgagtt gaatcagcag aaggattcgg tggcggggaa gtcgctccgg
     2701 gtcgggggca tggtcatgga tgggtcggtc aagtgggacc cgcagagcct cctgctgacg
     2761 ttcaagctga cggacggcca ggggacggtc ctggtgcgcc acaaggggac ggccccggac
     2821 ctgttcaagg aaggggctgg ggccgtggtg gaggggacct ggtcgcccga cggctacttc
     2881 cggtccagca ccatcatggc caagcactcc gaggagtaca agccgccgac gcatccggaa
     2941 accctggagt cgaagaaaga gatgttcaaa tccattctga agaagtgagg ggcatgatgg
     3001 tctccacgtt gggcggtttc gtactcttct tggcgctggg cgtgacgatc tacgccgccg
     3061 ccgcgtccgt gatcggggca cgccccaacc gtgaggattg gctggccagc tcgcgcaacg
     3121 cgatcctggt tcacttctgt ctggtcaccc tggcgatctt cctgctggag tacgcgctga
     3181 tcaccagcga cttcagcctg cgctacgtgg ccttcaacag caccagggga agcctcacgc
     3241 gatacaagat cgctggcctg tggggctccc tggagggctc catcctgctg tgggaatggc
     3301 tccaggcgct catgtcggcc ctggtggtgg cccgctatca tgacaagcac cggcagctca
     3361 tgccgtacgt ccaggcggtg cttcagggaa tctcgatctt cttcctgctg ctcatgacct
     3421 ttgccgtcaa tcccttcgcg cgcatcttcc ccatcccgcc ggacggccgc ggtctgaacc
     3481 ccctgctgga agtgaccgac atgctggtcc accctctgct gctgtactcg ggttatgtgg
     3541 gcttctccgt cccctacgct ttcggcatgg cggccctgat cacggggcgt ccctcggagg
     3601 agtggctcag catcacgcgc cgctggaccg tggtggcatg gatgttcctc acctgcggga
     3661 tcttctacgg gggctggtgg tcgtaccgga ccctcgggtg gggcggctac tgggcctggg
     3721 atccggtgga gaatgcgtcc ttcatgccgt ggctcacggg tacggccttc atccactcgg
     3781 tgatgatcca gcagaggaag cgaatgctcc gggtctggaa tttggccctg gtcaccctga
     3841 cgttttccct ggtgatcttc gggacgttcc tcacgcggag cggtatcctg ggctcggtcc
     3901 acgccttcgc ggacggcccc gtggggatcc tcttcctggc ctttctgtcc ctggtgctgc
     3961 tgttctcctt cggtctcgtg gcctaccggt ccgatcagct caagggccac ggcgagctgg
     4021 attccatcgt ctcgcgggag agcgcgttcc tgctgaacaa cgtggtcctg gtgggcatct
     4081 gctttacggt gttcctgggg acgatctttc cgctgctgtc cgaggccatc cgggggacca
     4141 agatcagcgt ggggacaccg tattttaacc gggtggcggg ccccctgggc atggccctgc
     4201 tcctcttgat gggggttggc ccactcatcg cgtgggggcg cgcctcgttg aacaatctga
     4261 ggcggaactt cctgaagccc tcgctcctgg ccatcgtcgg cacgatcatc gtgtacatcc
     4321 tgggcgtgca cgaggatgcg gccctggcgg cgttcctgtt tgcgttcttt gtgattgcca
     4381 cggtgagctt cgagttcgtg atctcgacgc gaacccgggc c
//
LOCUS       DBNGZC010000716         3170 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_160915, whole genome shotgun sequence.
ACCESSION   DBNGZC010000716 DBNGZC010000000
VERSION     DBNGZC010000716.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_160915"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="AC16G0_12350"
     CDS             complement(<1..1003)
                     /locus_tag="AC16G0_12350"
                     /inference="COORDINATES: protein motif:HMM:NF016027.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR5100438.1"
                     /translation="MGHLRRCRTIAHSLVDQHKKLNVLILSGSPIIGRFSFRSRVDFV
                     RVPGIIKLRNGDYTSLSLHVKIEDTLALRASIIEHTARVFDPHLFLVDKEPLGLLGEV
                     RQTLAMLKRRGRCQLVLGLRDIMDEPESLEDEWNRKGVMPALERFYDEIWVYGLKRIY
                     DSVAAYQFPPRVAAKTKFTGYLRREPRPDVPVPHEVTAILQRGPCILVTPGGGGDGAD
                     LVDAVLSAYERFDDRLPWPALVVYGPFLPAHQREAFELRASQLRRITTLTFHEHLENL
                     MEGAAGVVSLGGYNTFCEILSLNKRSIMVPRVTPRAEQLLRADTARKLGLVQVIAPYE
                     "
     gene            1433..2461
                     /locus_tag="AC16G0_12355"
     CDS             1433..2461
                     /locus_tag="AC16G0_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008192165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5100439.1"
                     /translation="MAGPLLQVEDLRTTIYLDEGVVHAVDGVSFTLNRGETVALVGES
                     GAGKSMVAQSILRILPDNARIEGGRLLFSPSDEAPLDLAQLPPDGKTMRAIRGSAIGM
                     IFQEPMTSFSPVHTIGDQIGETVRLHRGASRAEARVQAIHALKAVGFRDPERKVDAYP
                     FELSGGLRQRAMIAMALACRPALLIADEPTSAVDVTIQIQILHLLVALQREYGMALLL
                     IAHDPGVVAYLADRVVVMYRGKVMETGPLADLYANPQHPYLQALFRSVPDLDLDESHR
                     LQAIPGTVPDPFLELQGCPFLSRCLEAEAELCGRAMPQLTRLTPDHWVACYKRGSVPE
                     PAHAAVKA"
     gene            2493..>3170
                     /locus_tag="AC16G0_12360"
     CDS             2493..>3170
                     /locus_tag="AC16G0_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014176708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5100440.1"
                     /translation="MAKPEPLLIVRELTKEFPLYQPSLLGFGRRRVGVVKAVDGVSFS
                     VQRGETLGLVGESGCGKTTTARLILRVLRPTRGEILLQADGRPLRMDQLEGKALRGIR
                     RHIQLVFQDPYSSLNPRMPVGEIIGEPLRLQHVGTPAEIGERVRALMGMVGLNIQHLR
                     RYPHSFSGGQRQRIGIARALALNPELLVCDEPVSALDVSVQSQVLNLLKDLQAELQLA
                     YLFISHNL"
BASE COUNT          547 a         1075 c         1012 g          536 t
ORIGIN      
        1 gctcgtacgg agcgatcacc tggaccaggc ccagcttgcg cgcggtgtcg gcgcgcagca
       61 attgctcggc ccggggcgtc acccggggca ccatgatgct gcgcttgttg agggagagga
      121 tctcgcagaa ggtattgtac cccccgaggc tgaccacgcc ggccgctccc tccatcagat
      181 tctcgaggtg ctcgtggaag gtgagggttg tgatccgcct gagctgggag gcccggagct
      241 cgaatgcctc gcgctgatgc gcaggaagga aaggtccata gaccaccagg gcaggccatg
      301 gcaagcgatc gtcgaatcgc tcgtaggccg agaggaccgc atccaccaag tccgctccat
      361 cgcccccccc acccggcgtg accaagatgc acgggcccct ctgcaggatc gccgtcacct
      421 cgtggggcac tggcacgtcc ggccgcggtt cccgtctcag gtacccggta aacttcgtct
      481 tggcggcaac cctggggggg aactggtagg ccgcgacgga gtcgtagatc cgcttcaggc
      541 cgtacaccca gatctcatcg tagaaccgct ccagggccgg catgaccccc ttgcggttcc
      601 actcgtcctc cagcgactca ggctcatcca tgatatcgcg gaggcccagc actagctggc
      661 agcggccgcg gcgcttgagc atggccagcg tctgacgcac ctcgccaagc agacccaggg
      721 gctccttgtc cacgagaaac agatgcgggt cgaagacacg cgccgtgtgc tcgatgatgg
      781 aggctcgaag cgccagggtg tcctcgatct tgacgtggag gctgagactg gtgtagtcgc
      841 cgttccggag tttgatgatc cccggaaccc ggacaaaatc cacgcgagag cggaagctga
      901 atcgaccgat gatgggtgag ccgctgagga tcaagacgtt gagcttcttg tgctgatcga
      961 ccagggagtg cgcaatggtc cggcagcggc ggaggtggcc caacccaaag gaatcgtggc
     1021 tgtagatgag gacccgatag ccggactcag accccgggga ccgacgctac gaactgccgt
     1081 cctgtgcccc cagggacttc accgccaccc gccgcatcac cattcgcctg acctccccgg
     1141 cgtgcgcgaa cccctgacgg ctgaggagca ttccagccac cgtcgggcct ttccccgacc
     1201 aagagacccg atgaccggca gagcctacta cgacttccgg cacaaaacaa gcgcaacatg
     1261 ttacgtctga tctgccaaac gttaccgccg ggaagattcc gcattgaccg tctgtgacgg
     1321 ggttttctgc gacggggttc tcttgacaga gtacggccgg gatggtatcc gtgacagaat
     1381 aggaaggcct ggcagcctgc gaggcagcgg cgctggattc tggagcacgg atatggctgg
     1441 tcccctgttg caggtggaag acctccgcac gaccatctac ctcgacgaag gggtggtgca
     1501 cgccgtggac ggcgtctcct tcacgttgaa tcgcggggaa accgtggccc tggtgggaga
     1561 gtcgggcgcc ggcaagagta tggtggccca atccatcctc cgcatcctgc cggacaatgc
     1621 ccggatcgaa ggcggccggt tgctgttctc cccctcagac gaggcgccgc tggatctggc
     1681 ccagcttccc cccgacggga aaaccatgcg ggcgatccgc ggcagcgcca tcgggatgat
     1741 cttccaggag ccgatgacct cgttttcacc ggtgcacacc atcggcgatc agatcggcga
     1801 aacggtccgg ctccaccggg gggcgagtcg cgccgaggcc cgcgtccagg ccattcacgc
     1861 gctcaaggct gtgggatttc gcgacccgga acgtaaggtg gacgcctatc ccttcgagct
     1921 gtccggtggt ctccgccaac gggccatgat tgccatggcg ctggcctgcc ggcccgccct
     1981 cctgatcgcg gacgagccca cctcggcggt ggatgtgacc atccagatac agatccttca
     2041 cctgctcgtc gccctccagc gcgagtacgg catggccctc ctgctgatcg cccacgaccc
     2101 cggcgtggtc gcgtaccttg cggaccgcgt ggtcgtcatg taccgcggga aggtcatgga
     2161 gacgggccct ctggccgatc tgtatgcaaa cccgcagcat ccctacctcc aggccttgtt
     2221 ccggagtgtg ccggacctcg acctggacga aagccaccgg ctccaggcta tccccgggac
     2281 ggtcccggac cccttcctcg agctgcaggg ctgcccgttt ctctcccgct gcctggaggc
     2341 ggaggcggag ctgtgcggcc gggccatgcc ccaactcact cgcctcaccc ccgatcactg
     2401 ggtggcgtgt tacaagcgcg gctccgtgcc cgaaccggcg cacgccgctg tgaaggcctg
     2461 aggcattgac ggcgctcctg gtggggacac ctgtggcaaa acctgaaccc ctcctgatcg
     2521 ttcgggagct cacaaaggaa ttccccctgt atcaaccctc gctcctggga ttcggccgcc
     2581 gccgggtggg ggtggtcaag gccgtggacg gcgtgagttt cagcgtgcag cggggggaga
     2641 ctttgggcct ggtgggcgag agcggctgcg gcaagaccac gaccgcccgg ctgatcctgc
     2701 gggtcctgcg gccgacccgc ggcgaaatcc tgctccaggc cgacggccgc ccgctgcgca
     2761 tggatcagct cgaaggaaag gccttgcggg ggatccgccg tcacatccag ctcgtcttcc
     2821 aggaccccta ctcctcgctc aacccgcgca tgccggtggg ggagatcatc ggggaacccc
     2881 tccgattgca gcacgtcggc acgcccgcgg agatcggcga gcgggtccgg gccctgatgg
     2941 ggatggtcgg actcaacatc cagcacctcc ggcggtaccc ccattccttc tccgggggtc
     3001 aacgccagcg gatcgggatc gcccgcgccc tggccctcaa ccccgaactg ctggtgtgcg
     3061 acgagccggt gtccgccctg gacgtctccg tccagtccca ggtgctgaac ctcctgaagg
     3121 acctccaggc tgagctgcag cttgcctacc tcttcatctc ccacaacctc
//
LOCUS       DBNGZC010000717         1300 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_844727, whole genome shotgun sequence.
ACCESSION   DBNGZC010000717 DBNGZC010000000
VERSION     DBNGZC010000717.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1300)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_844727"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(383..>1300)
                     /locus_tag="AC16G0_12365"
     CDS             complement(383..>1300)
                     /locus_tag="AC16G0_12365"
                     /EC_number="4.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048040 - UDP-glucuronate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HIR5100441.1"
                     /translation="GAGFLGSHVCDRLLQEGQEVICADNLLTGNMDNIAHLMGREGFR
                     FIKQDVTEYLYVEGPLDFILHFASPASPVDYLRFPIQTLKVGSLGTHKALGLAKAKGA
                     RFLLASTSEVYGDPQIHPQPESYWGHVNPVGPRGVYDEAKRFAEAMTMAYHRYHGVQT
                     RIARIFNTYGPRMRPNDGRVVSNFLVQALHGQPLTVYGDGRQTRSFCFVSDLVEGLFR
                     LLVSSEVDPVNLGNPAERTIRDLVTAIEGILGHSLPVTYNPLPVDDPRVRQPDISRAR
                     ACLGWEPTVDLDAGLRKTIADFRKNGVGV"
BASE COUNT          242 a          429 c          412 g          217 t
ORIGIN      
        1 cgccgaaacc ggcccagata tttgcccagc gtcgtgcggc tcatgcgtct gtaactcctc
       61 gtcaaaacgt cgcctaagct atctcatttc catcgagaat tcaacccgct tctcccgcca
      121 gacatccccg caaagagcca tcgggaggca aaagaatggc ggcgagggag agtctccctc
      181 gccgccatgg cggtaaccca ggctgactga atgactcgga tggcctccgc aggaattccc
      241 ctaccctcct tgagggaagt gtctctcgaa cggtcgagat gatatcagat tcctcgctgc
      301 cggacaagcg ccccggaatc gttcctacca agccactgaa aaacctgcgg cggacgatga
      361 ccgggaccga ggagccggtg cctcagactc ctacaccgtt cttccggaaa tcggcaatcg
      421 ttttcctgag accggcatcc aggtccaccg tgggttccca ccccaggcag gcgcgggcgc
      481 ggctgatgtc gggttggcgc acccgggggt cgtccacggg cagcggatta taggtgaccg
      541 gcagcgagtg gcccaggatc ccctcgatgg ccgtcacgag gtcccggatc gtgcgctccg
      601 caggattgcc caggttgacc ggatccacct ccgaggacac gaggagccgg aacaggccct
      661 ccaccagatc ggagacgaag cagaagctgc gcgtctgccg gccgtcgcca tacaccgtga
      721 gcggctggcc atgcagcgcc tggaccagga agttggagac cacccggccg tcgttggggc
      781 gcatccgcgg cccataggtg ttgaagatcc gggcaatccg ggtctggacc ccgtgatacc
      841 ggtggtaggc catggtcatg gcctcggcga accgcttcgc ctcgtcgtac accccccggg
      901 ggcccaccgg gttcacatgg ccccagtagc tctcgggttg cgggtggatc tggggatccc
      961 cgtacacctc cgaagtggag gccaggagga accgcgcccc cttggccttg gccaggccca
     1021 gggccttgtg ggtgccgagg gagccgacct tcaacgtctg gatggggaag cgcaggtagt
     1081 ccaccggact ggcgggggag gcgaagtgca ggatgaagtc cagcggcccc tcgacgtaca
     1141 ggtactcggt cacatcctgc ttgatgaacc ggaagccctc gcgacccatg aggtgggcga
     1201 tgttgtccat gttcccggtg agcaggttgt cggcgcagat cacctcctgc ccttcctgga
     1261 ggagccgatc gcacacgtgc gaccccagga accccgcccc
//
LOCUS       DBNGZC010000718         6689 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2017207, whole genome shotgun sequence.
ACCESSION   DBNGZC010000718 DBNGZC010000000
VERSION     DBNGZC010000718.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6689)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6689)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6689
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2017207"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="AC16G0_12370"
     CDS             <1..311
                     /locus_tag="AC16G0_12370"
                     /inference="COORDINATES: protein motif:HMM:NF012497.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100442.1"
                     /translation="GVVITAAEDSLRIAGGRPLRGAAVSSRGDHRMAMSLAIAGLYAD
                     GETTVKDVACVETSFPGFAHLLEMVAPSCGIREMQDAERGPMRDTPQRTRRSEEVPVP
                     "
     gene            308..1003
                     /gene="cmk"
                     /locus_tag="AC16G0_12375"
     CDS             308..1003
                     /gene="cmk"
                     /locus_tag="AC16G0_12375"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020373129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR5100443.1"
                     /translation="MISRRSRLVIAIDGPVGAGKSTAARRLAEALGSVYIDSGAMYRG
                     MGWKAIHEGVDLRDHAGLSRLAARTDIRVVAGPTGPRVLVDGLDVTAALRTPRIDEAS
                     SLVSTCGEVRQRLVALQRAMSRQGGVVMDGRDIGTVVFPDAYLKFYLDADLSIRASRR
                     LNDLRHAGAQADLSTVQKEVEQRDRRDTGRTLSPLRVAPDAVRIDSSTLDADEVLGVM
                     LNAVRKIVEPDNS"
     gene            1102..1731
                     /locus_tag="AC16G0_12380"
     CDS             1102..1731
                     /locus_tag="AC16G0_12380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR5100444.1"
                     /translation="MIYRIGWRLCRWIAQGFFQLQIEGVEHVPERGPVILAPNHVSYL
                     DPILVGVSIRRRAHFMAKKELFKNRLAAWILRSVQAYPVTRERLDPSTLKHALSLLSR
                     GELLLIFPEGTRGDGRRLGPAKPGLAAIAARSGASVVPVFHWGTERALPRKSRRIRRV
                     PLRVRFGPPLRVAGNGETSRQALEVFGQQVMDAISALRPAEPPWISDSQ"
     gene            1834..3549
                     /locus_tag="AC16G0_12385"
     CDS             1834..3549
                     /locus_tag="AC16G0_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR5100445.1"
                     /translation="MTSQLVGSEDQSSTEFEESEHSGPSAEMQEIYEQSFRDIVEGDI
                     IKGTILELRDDAVLVDIGYKSEGLIPLREFRTPSGEITAKIGDSVDVYLEQKEDSDGL
                     IVLSREKAEKIKIWDELSKIYEKAGAVTGTILGRTKGGLIVDIGVRAFLPGSQVDLRP
                     VRDLDKLIGKAFPMKIIKLNQKRGNIVLSRRELLEEERKQLKEQTLTNLEEGKRIKGK
                     IKNITEYGAFVDLGGLDGLLHITDMSWGRVGHPSELFQVGDEIEVVVLKFDRSNERVS
                     LGYKQRLPDPWAAVEERYPVGSKLKGKVVSLTDYGAFVELEEGIEGLVHISEMSWTQR
                     VKHPSKVVAIGDVVEVVVLDVDRANKRISLGMRQAEPNPWNFIEERYPVGTRVEGKVR
                     NLADFGAFVELEEGIDGLIHISDMSWTKRVRHPSEVLKKGEKVEAIVLHVDKANHRIS
                     LGLKQIQQDPWQSTVPEKYRVGMDVQGKVIRTTDFGAFVELEDGVEGLLHVSELSHER
                     VNKPEDVVQVGQDLTLRVIKLDPEERKIGLSLRAYLEGQRESTPDDEGGHGRKRRRRE
                     GAEEE"
     gene            3595..3876
                     /locus_tag="AC16G0_12390"
     CDS             3595..3876
                     /locus_tag="AC16G0_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR5100446.1"
                     /translation="MTKADLVEKVATHVNLTKKDTEVVIDTIFDSISQALAAKTDAKV
                     ELRGFGSFRVRRRRARQGRNPQTGKPVDVPAKRVPYFKPGKELKALVDS"
     gene            3979..5850
                     /locus_tag="AC16G0_12395"
     CDS             3979..5850
                     /locus_tag="AC16G0_12395"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR5100447.1"
                     /translation="MILETLANCEPRFIQGTARELEELFGSRGLELPEAQLAPEETDA
                     LLVDLHPGLSQGLRDLIESIYRILSERGGEKDPSLDRRRAESPFRRFEEILEAVLTTI
                     IERERRLGLLNLFWLAHSKHAAGLIQEVFSRPGFKITIKYQMHPLLQGAYRNAFARVW
                     TRFKSQGGATLRRNLGGNFNPSLIDCIIDDQLPLTETSLARLNFTTVLVENNKRFRIS
                     FREFIEIYTACRERLREGVQRKESRLLEIVRHALPSIRPDLYEDEHGATRILFNSRVV
                     TYLLGDVGGLGSRLLANPILKTALTARRGWGDLLMDYLDLLQAVKRSEVVDLARQALS
                     LVGPGHTEIELRERSDQGRLYRFHPGSEVLKLARKVTVLFADLRGFTQTSEGGVSEVE
                     LTERLYEVFDPLASIVERYHGRIDKFTGDGVMITFGSARATRQDELNALRTALAMQQT
                     MTELRAAGRTRFEMGISVHTGRAQLAHFIVDDETMDRTVIGRNVNIAGRLSGSGKSEA
                     SPFADQGDRVERGVGATQSAARDVWVDEEGTLYNRGIVVSQDTVEELHKQVEAEPLAG
                     AQGGGYRFRDEILRKNVLLEYRGDAKFKGVGRSIAIYRLGLEGGQPQGSSGPSREGQ"
     gene            5847..6338
                     /locus_tag="AC16G0_12400"
     CDS             5847..6338
                     /locus_tag="AC16G0_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010867189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR5100448.1"
                     /translation="MTSTLWAPWRMAYIGGARREGCLLCDVPSAQADRANLVLHRGPL
                     TYVVMNLYPYNNGHLMIVPYRHCAGLGQLSPEESLEIMQAAQRSTQVLETAFGAEGFN
                     IGFNLGKAAGAGIAEHVHLHVVPRWVGDTNFMPVLADTKVMPDYLEATYAKLVPLFQE
                     QGR"
     gene            6349..>6689
                     /locus_tag="AC16G0_12405"
     CDS             6349..>6689
                     /locus_tag="AC16G0_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HIR5100449.1"
                     /translation="MLIKMTVRGIALDPITNMPIIILKDVDERKALPIWVGIFEANAI
                     ALELEKVSTPRPMTHDLLKSILDGLGVTVRQVIVNDLKENTFFAVIELQHNGNTINID
                     ARPSDAIALALR"
BASE COUNT         1274 a         1990 c         2285 g         1140 t
ORIGIN      
        1 tgggggtcgt catcaccgcg gcagaagaca gcctgcggat agcgggcggg cgacccctac
       61 gaggagccgc cgtgagtagc cgcggcgatc accgcatggc catgagcctg gccatcgccg
      121 gcctgtacgc cgacggggag acgacggtga aggatgtggc ctgcgtggag acgtcattcc
      181 ccggcttcgc ccacctgctg gaaatggtcg caccgagctg cggtattcgc gagatgcagg
      241 acgcggagcg cgggccgatg agggacacgc cgcagcgcac tcgacgatcc gaggaggtgc
      301 ctgtgccttg atttcacggc ggtcccgact ggtcatcgcc atcgatgggc ctgttggggc
      361 gggcaagagc acggcagcgc gccgcctggc agaggccctg ggctctgtgt atatcgacag
      421 cggcgccatg tatcgcggca tgggctggaa ggccatccat gagggtgtag acctccggga
      481 ccatgcgggc ctgtcgcgcc tggccgcccg gacagacatc cgagtggtgg ccgggccaac
      541 gggcccgcgg gtgctggtgg acggcctgga tgtgacggcg gcgctccgga cccccaggat
      601 cgacgaggcc tcttccttgg tgtccacctg cggagaggtc cgtcagcggt tggtggcgct
      661 ccagcgggcc atgtcccgac agggcggggt cgtcatggac ggccgggaca tcggaacggt
      721 ggtcttcccg gatgcctacc taaagttcta tttggacgcc gatctctcga ttcgcgcctc
      781 ccgccgtttg aatgacctgc ggcacgcggg ggcccaagcc gacctgtcca ccgtccagaa
      841 ggaagttgag caacgcgacc ggagggacac ggggcgcacg ctctcgccgc tccgtgtcgc
      901 cccggacgcg gtccggatcg attccagtac actggatgcg gatgaggtcc ttggggtgat
      961 gttgaacgca gtccggaaga ttgtggaacc ggataactcc tgaattttca gggagatgcg
     1021 gattattttt cttgcctctg ccggtgtggc cgtgtatgat cccccgtccc tcgtggcccg
     1081 tggcggagag ggttgccggg gatgatctac cggatcgggt ggcggctatg tcgctggatt
     1141 gcccaagggt tcttccaact ccagatagaa ggggtcgagc acgtgcccga aagggggccg
     1201 gtgatcctgg cccccaatca tgtcagctat ctggatccca tactcgtggg cgtctccatc
     1261 cgtcgccggg cccatttcat ggccaagaag gagttgttca agaatcggct ggcagcgtgg
     1321 atcctccgta gcgtgcaggc gtatccagtg acacgggagc gcctggatcc ttccacactg
     1381 aagcatgcgc tctccctact cagccgcgga gagttgctcc tgatcttccc ggagggaacc
     1441 cggggggatg ggcgcaggct gggccccgcg aagcccggac tcgccgcgat cgcggctcgc
     1501 tccggtgcat cggtggtgcc ggttttccac tggggcactg aacgagcgct cccccggaag
     1561 agccgccgga ttcggcgggt gccgctgcgg gtgcggttcg gtcccccgct ccgagttgcc
     1621 ggcaacgggg agaccagccg tcaggcgttg gaggtgttcg gccagcaagt catggatgcg
     1681 atctcggcgc tccggccggc ggagccgccg tggatttcgg attcccagtg aggcggcatg
     1741 gtcgcctcga aaaggccagt caccgtgctc cgcatggcgg agacgggacg gcggttctaa
     1801 ttcacggccg cgtgcagcga ggagtaggtt cgtatgacat cacagttagt cggttcagaa
     1861 gatcaatctt ccacggagtt cgaagagtcc gagcactcgg gcccttcggc ggagatgcag
     1921 gagatctacg agcagagctt tcgagacatt gttgagggtg acatcatcaa ggggaccatc
     1981 ctggagcttc gcgacgacgc ggtcctggtg gacatcggct ataagtccga gggcttgatc
     2041 cccctgcggg agttccgcac gccttccgga gagatcacgg ccaagatcgg cgacagcgtg
     2101 gacgtgtacc tggaacagaa ggaagacagc gacggcctga tcgtgctgtc gcgggagaag
     2161 gccgagaaga tcaagatctg ggacgagctg tccaagatct acgagaaggc gggcgcggtc
     2221 acagggacga tcctgggtcg gaccaaaggg ggtctgatcg ttgacatcgg cgtccgggct
     2281 ttccttcccg gctcccaggt cgacctgcgc ccggtacggg acctggacaa gctcatcggg
     2341 aaggcgttcc cgatgaagat catcaagctg aaccaaaagc gagggaacat cgtcttgtcc
     2401 cgacgcgaac tcctggaaga ggagcggaag cagctcaagg aacagacgct caccaacctg
     2461 gaggaaggga agcgcatcaa ggggaagatc aagaacatca ccgagtacgg cgccttcgtg
     2521 gatctgggcg gcctggatgg gttgctccac atcaccgaca tgtcctgggg gcgcgtgggt
     2581 catccctccg agctgttcca ggtcggggac gagatcgagg tggtcgtgct gaagttcgat
     2641 cggagcaacg agcgggtgtc gctcggttac aagcagcggc tgccagatcc ctgggcagcc
     2701 gtggaggagc ggtatccggt cggctccaag ctcaagggga aggtggtcag cctcaccgat
     2761 tatggcgcct tcgtcgagct ggaggagggg attgaagggc tggtccacat ctccgagatg
     2821 tcgtggaccc agcgggtcaa gcatccgtcc aaggtcgtgg ccatcggcga cgtggtcgag
     2881 gtggtggtac tggatgtgga ccgggccaac aagcgcattt cgctgggaat gcgccaggcc
     2941 gagccgaacc cctggaattt catcgaagag cgctatcccg tggggacccg cgtcgaggga
     3001 aaggttcgga acctcgccga cttcggcgcc ttcgtggagc tggaagaggg gatcgacggg
     3061 ctcatccaca tctccgacat gtcgtggacc aaacgcgtgc ggcatccgtc cgaggtgctg
     3121 aagaaagggg agaaggtgga agccatcgtc ctccatgtgg acaaggccaa ccaccgcatc
     3181 tcccttggct tgaaacagat ccagcaggac ccctggcagt cgaccgtgcc cgagaaatac
     3241 cgggtcggga tggacgtgca gggcaaggtg atccgcacca ccgacttcgg tgccttcgtg
     3301 gaactggagg acggggtgga ggggctcctg cacgtctcgg agctttccca tgagcgcgtg
     3361 aacaagcccg aggatgtggt gcaggtcgga caggacctga cgctgcgggt catcaagctc
     3421 gacccagagg agcgaaagat cggtctcagc ctccgggcct acctcgaggg gcaacgagag
     3481 agtaccccgg acgacgaggg tggacacggg cggaagcgac ggcggcgtga gggtgccgag
     3541 gaagaatagt cgaggggccg ttggggtggc ccatcctcaa aggaggtgga tttcatgacc
     3601 aaagccgatc tggtggaaaa ggtcgccacg cacgtcaacc tgaccaagaa ggatacggaa
     3661 gtcgtcatcg atacaatctt tgacagcatc tcccaggccc tggccgccaa gactgacgcc
     3721 aaggtcgagc ttcgaggttt cgggagcttt cgggtgcgca ggcgccgcgc ccggcagggg
     3781 cggaatccgc agaccggtaa gccagtggac gtcccggcca aacgtgtgcc gtacttcaag
     3841 cctggcaaag agttgaaggc cctggtggat tcctaactcg ccgcgcgata cgggacggaa
     3901 agagcagggc cgcgttgccc tgctttttca ttggcggtgg cggaggggac ggcggttacc
     3961 ccgcgggagt ccaggcggat gatcttggag accctagcca actgcgagcc ccgcttcatc
     4021 cagggaacgg cccgggaact ggaggagttg ttcggcagcc gcgggctgga gctacccgag
     4081 gcgcaactcg ccccggagga gacggatgcc ctgctcgtgg atctccatcc cggcctctcc
     4141 cagggtctcc gggacctcat cgagtccatc tatcgcatcc tgtccgagag ggggggcgaa
     4201 aaagacccct ccttggatag gcgccgcgct gaaagtccct tccgccgttt tgaagagatc
     4261 ctggaggccg tgctgaccac catcatcgag cgcgagcgcc gcctcgggct gctcaatctg
     4321 ttctggctgg cccattccaa gcatgccgcc ggtctcatcc aggaggtttt ctcgcggccc
     4381 gggttcaaga tcaccatcaa gtatcaaatg catccactcc ttcagggcgc gtatcgtaac
     4441 gccttcgcgc gggtctggac gcgcttcaag agccaggggg gggccaccct ccgtcgcaac
     4501 ctggggggca acttcaatcc cagcctcatc gactgcatca tcgacgacca gcttcccctg
     4561 acggagacca gcctggcgcg gctgaacttc acgaccgtcc tggtcgagaa caacaagcgg
     4621 ttccgcatca gttttcgaga gttcatcgag atctacacgg cctgccgaga gcgtctacga
     4681 gagggggtgc agcggaagga gtcccgcctc ttggagatcg tgcggcatgc gctgccctcc
     4741 atacgccccg acctatacga ggacgagcac ggagcaaccc ggatcctgtt caacagccgg
     4801 gtggtgactt acctgctggg cgacgtcggc ggcctgggaa gccgactgct ggcgaatccg
     4861 atcctcaaga ccgcgctgac ggcgcggcgg ggctggggtg acctcctcat ggactacctg
     4921 gacctgctgc aggccgtcaa gcgcagcgag gtcgtggact tggcgcgcca ggcgctgtct
     4981 ctggtcgggc cgggacacac tgaaatcgag cttcgagaac gcagcgacca gggacggctc
     5041 taccgtttcc atccgggcag cgaggtgttg aagctggccc ggaaagtcac ggtgctcttc
     5101 gcagacctcc gcggcttcac gcagacctcc gagggcgggg tctcggaggt cgagctgacg
     5161 gagcggttgt acgaggtctt cgacccgctg gcctccatcg tggagcggta tcacggacgc
     5221 atcgacaagt tcaccgggga cggcgtgatg atcaccttcg gctcggcccg agccacccgg
     5281 caggacgagc tgaatgcctt gcgtacggcc ctggccatgc aacagacgat gacggagctg
     5341 cgggctgccg gacggacccg cttcgagatg ggcatcagcg tccacacggg acgggcccag
     5401 ctggcccact tcatcgtcga cgatgaaacc atggaccgca ccgtcatcgg gcggaacgtg
     5461 aacatcgcag gccggttgtc gggttcggga aagagcgagg cttccccctt tgcggaccag
     5521 ggcgaccgtg tggagagggg agtcggggcg acccagtcgg cagcgcggga cgtctgggtg
     5581 gacgaggagg gaaccctgta caaccggggg atcgtggtca gccaggacac agtggaggag
     5641 ctgcacaagc aggtggaggc cgaaccgctg gcaggcgcgc aaggcggcgg gtatcgattt
     5701 cgcgacgaga tcctgcgaaa aaatgtcttg ctagagtata ggggcgatgc taaattcaaa
     5761 ggcgtcggcc ggtcaatcgc gatataccgg ctgggcctgg aggggggcca gccccagggg
     5821 tcgtcggggc cgtcgcggga gggacaatga cctcgacgct ctgggcgccg tggcgcatgg
     5881 cctatatcgg tggggcgcgc cgagaagggt gcctgctgtg tgacgtcccg agcgcgcagg
     5941 ctgaccgagc gaatctggtt ctccatcgcg ggccgttgac gtatgtggtc atgaacctgt
     6001 acccgtataa caacggccac ctcatgatcg tgccttaccg acactgcgcg ggtctgggcc
     6061 agctctcccc cgaggaatcc ctggaaatca tgcaggcagc acagcgatct acccaggtcc
     6121 tggagaccgc attcggggcc gagggcttca atatcggctt caacctggga aaggccgccg
     6181 gcgctggcat cgcagagcat gtccacttgc acgtggtgcc gcgatgggtg ggtgacacca
     6241 acttcatgcc cgtgctggcg gacacaaagg tcatgccgga ctacctggaa gccacctacg
     6301 caaagctggt tcccctcttc caggagcagg ggaggtgagg agggccccat gctcatcaag
     6361 atgaccgtgc gggggatcgc cctggatccc atcacgaaca tgcccatcat catcctcaag
     6421 gacgtggacg agaggaaagc cctccccatt tgggtcggca tcttcgaggc gaacgccatc
     6481 gccttggagc tggagaaggt ctccaccccc cgacccatga cccacgactt gctcaagagc
     6541 atcttggacg ggctgggagt gaccgtccgg caagtgattg tgaacgacct caaagagaac
     6601 accttcttcg ccgtgatcga gctgcagcac aacgggaaca cgatcaatat cgacgcccgc
     6661 ccaagcgatg ccatcgccct ggcccttcg
//
LOCUS       DBNGZC010000719         1989 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2117895, whole genome shotgun sequence.
ACCESSION   DBNGZC010000719 DBNGZC010000000
VERSION     DBNGZC010000719.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2117895"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="AC16G0_12410"
     CDS             <1..443
                     /locus_tag="AC16G0_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR5100450.1"
                     /translation="QERDKIAALDAGADDYVSKPFGVGELLARMRVALRHLAHAPGDP
                     GESTFAVGDLQVDLVRRQVLVAGAPIHLTPIEYRLLTTLIHHAGKILTHQHLLREVWG
                     PHQTDQAQYLRVYVAQLRRKLERNPARPRYVLTEPGVGYRLAAE"
     gene            587..>1989
                     /locus_tag="AC16G0_12415"
     CDS             587..>1989
                     /locus_tag="AC16G0_12415"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR5100451.1"
                     /translation="MASSLKRIILGRPLESAAERHERLGKIAGLAVFASDALSSVAYG
                     TEEVLLVLVTAGSAALSLSYPIMVGIAILVGIVAASYWQTIHAYPSGGGSYIVAKDNL
                     GTLPGLIAGASLLIDYVLTVAVSTASGVAAITSAVPAFFPWRVELCVICVTLVTIANL
                     RGVRESGRLFTVPTYWFIGSMFLLILAGFLRVATGTTQPPPLVEVQATQALTLFLILR
                     AFSSGCATLTGIEAVSNGIPAFRPPEPRNASTTLVWMAAILAATSLGIAYLAHAYHIV
                     PSESETVVSMLARNAFGRNLLYYNIQASTALILFLAVNTSYQDFPRLSSILARDGFMP
                     RQMANRGDRLVFSNGIIILAGLSILLLVAFHGETHALIPLYAVGVFLSFTLSQSGMVR
                     HWLKEERPSPHHIALNGLGALTTGVVTVVIAVSKFTHGAWMVIVLIPLIVLALRRVQG
                     HYQQVAQRLSLQGANRPR"
BASE COUNT          314 a          685 c          576 g          414 t
ORIGIN      
        1 ggcaagagcg agacaagatc gcggccctgg atgcgggtgc ggacgactac gtcagcaagc
       61 ccttcggggt tggagaactg ctcgcccgta tgcgcgtggc cttgcgccac ctcgcgcacg
      121 cgccgggcga cccgggcgag tccaccttcg ccgtcggcga cttgcaggtg gatctcgttc
      181 gcaggcaggt cctggtggca ggggctccca ttcacctcac cccgatcgag taccggctcc
      241 tcaccaccct catccatcat gcaggcaaga ttctgacgca ccagcacctc ctccgagagg
      301 tctggggacc ccatcagacc gatcaggcac aatacctgcg ggtgtacgtc gcccagcttc
      361 ggcggaaact ggagaggaac cctgcccggc cgcgctacgt cctcaccgag ccgggggtgg
      421 ggtaccggct tgccgcagag tgatgccctc ccccaatcgc ccgatttttc cgcacgcggg
      481 atcacatcac acacggccta tcgcaacttg acactctgcg cacacgagac tagcatacgc
      541 gcctgtccgc aggggttccc ttgcggaccc tctatatgga gggcttgtgg cgagttccct
      601 gaaacggatc atccttggca ggcccctgga gtcagccgcc gagaggcatg agcgactggg
      661 caagattgcc gggctggcgg tctttgcgtc ggacgccttg tcctccgtgg cttacggcac
      721 ggaggaagtg ctcctcgtcc tggtgacggc gggctccgca gccctctccc tctcatatcc
      781 catcatggta gggatagcca tcctcgtcgg gatcgtggcg gcgtcctact ggcagacgat
      841 ccatgcgtat ccttcgggcg ggggttccta tatcgtcgcc aaggacaacc tgggcaccct
      901 tccgggtctt atcgccggtg cgtcgcttct catcgactat gtcctgactg ttgcggtgag
      961 caccgcctcg ggtgttgccg cgattacgtc tgctgttcct gcattctttc catggcgcgt
     1021 cgaactctgc gtcatttgtg taacgctggt caccatcgca aacctccggg gggtgagaga
     1081 gtccgggcgg ctcttcacgg tcccaacata ctggttcatc ggtagcatgt ttctgctcat
     1141 cctcgcgggc ttccttcgag tcgccactgg aactactcag ccgcctccgc tcgttgaggt
     1201 ccaggcgacg caggccctca ccctcttcct gatcttgcgc gctttctcct cgggttgcgc
     1261 taccctcacg ggcatcgaag ccgtgagcaa tggaatcccc gcgttccgac caccggaacc
     1321 gcggaacgcc agtacgacct tggtgtggat ggcggcgatc ttggcggcga catccctagg
     1381 gatcgcctac ctggcccacg cgtatcatat cgttccgagt gaatcggaaa ccgtcgtctc
     1441 gatgctggcc cggaatgcct ttgggcggaa cctgctctat tacaatattc aggcctccac
     1501 ggccttgatc ctgtttctgg ccgtaaacac ctcataccag gatttccctc gactctcctc
     1561 catcctggcc cgagacgggt tcatgccgcg gcagatggcg aaccgcggtg accgcctggt
     1621 cttcagcaac gggatcatca tcctggcggg gctgtccatc ctcttgctgg tggccttcca
     1681 cggcgagacc cacgccctga tccccctcta cgccgtcggg gtcttcctct ccttcaccct
     1741 ctcccagtcc ggtatggtcc ggcactggct gaaggaagag cgcccctcgc ctcaccacat
     1801 cgccctgaac ggactcgggg ctctcaccac cggtgtcgtg accgtggtca tcgcggtttc
     1861 caagttcacc cacggggcct ggatggtgat cgtcctgatc ccgctgatcg tcctcgcgct
     1921 tcgccgggtg cagggtcact accagcaggt cgcccaacgg ctctcgctgc agggcgccaa
     1981 tcggccccg
//
LOCUS       DBNGZC010000720         1877 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1724318, whole genome shotgun sequence.
ACCESSION   DBNGZC010000720 DBNGZC010000000
VERSION     DBNGZC010000720.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1724318"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1672)
                     /gene="purH"
                     /locus_tag="AC16G0_12420"
     CDS             complement(53..1672)
                     /gene="purH"
                     /locus_tag="AC16G0_12420"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017507565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR5100452.1"
                     /translation="MMRVRRALLSASNKVGLVDFAAGLMRLGVQLVSTGGTARVMREN
                     GLPVTDVTEITEFPEMLDGRVKTLHPRIHGGLLGIRGKPEHERQMAEHGILPIDLVAI
                     TLYPFGEATARPDVSLHEAIENIDIGGPAMLRSAAKNFEAVAVILDPADYEPVLAEMQ
                     AAQGTLSRATRFALARKAFAHTARYDALISAFLDRVELSPDSTDIERSSPRLRTPTDG
                     QGDGLPRTLTLRLEKLQDLRYGENPHQRAAFYRDLDAPGGIAAARQLHGKELSYNNIV
                     DLHAAWALVQEFDAPVVAIIKHTNPCGVATAPDLRSAYSRARATDPVSAYGGIIGVNR
                     PLDVAAAREIGGSFVEAVIAPGFAVEALDLLKQKKNIRLLEIPPAEEQRASDMRQYDM
                     RHVSGGMLVQDPDGADTDPAGLKVVTKRSPTSEETEAMRFAWKVAKHVKSNAIVLASE
                     SATVGIGAGQMSRVDSAKLAVMKANFPTQGTALASDAFFPFRDGVDAAAGAGVTAIIQ
                     PGGSVRDGEVIAAADEHGIAMLFTGIRHFRH"
BASE COUNT          301 a          625 c          616 g          335 t
ORIGIN      
        1 accaggactc gcatgtcagc tccgtctcgt cctcgccagg cttcgagcat cgtcaatggc
       61 ggaagtgccg aatacctgtg aacagcatgg cgatgccgtg ctcgtctgca gcggcgatga
      121 cctcaccgtc gcgcaccgac ccgccgggct ggatgatcgc ggtgaccccc gcccccgccg
      181 ccgcatccac accgtcgcgg aaggggaaga aggcatccga ggcgagggca gtcccctggg
      241 tggggaagtt cgccttcatc accgccagct tggctgaatc gacccggctc atctggccgg
      301 ccccgatgcc caccgtagca gactccgagg cgaggacgat ggcgttcgac ttcacgtgct
      361 tcgccacctt ccaggcaaat cgcatggcct cggtctcttc cgaggttggg gagcgcttgg
      421 tcaccacctt cagcccggca ggatccgtat ccgctccatc cggatcctgg accagcatcc
      481 ccccactcac gtggcgcatg tcgtattggc gcatgtcgga tgcccgttgc tcctcggctg
      541 gcggtatttc caggaggcga atgttcttct tctgtttgag gaggtccagg gcctctacgg
      601 caaaccccgg cgcgatgacg gcctccacga agctgccgcc gatctcgcgg gccgcggcga
      661 cgtccagggg ccggttgact ccgatgatcc ctccgtatgc cgagaccgga tcggtcgccc
      721 gggcccggct ataggcgctc cggaggtcgg gggcggtggc cacgccgcag gggttcgtgt
      781 gtttgatgat ggccacgacc ggcgcatcga actcctgcac gagagcccag gcggcgtgca
      841 ggtccacgat gttgttgtac gagagctcct ttccatggag ctgtcgagcg gcggcgatcc
      901 cccccggcgc atccaggtcc cggtagaagg cggcgcgctg atgcggattc tcaccgtagc
      961 gcagatcctg gagcttttcc agccggaggg tcagggtgcg cggcaagcca tccccctgcc
     1021 catccgtcgg agtccggagt cgtggggacg atctttcgat gtccgtggaa tccggagaga
     1081 gttccacgcg atccagaaag gcgctgatga gcgcgtcgta gcgcgcggtg tgggcgaagg
     1141 ccttgcgcgc caaggcaaac cgcgtcgccc gggagagagt tccctgagcg gcctgcattt
     1201 ctgcgaggac gggctcgtag tcggctgggt ccaggatcac ggcgaccgct tcaaaattct
     1261 tggccgccga acgcaacatg gcggggccgc cgatgtcgat gttctcgatg gcttcgtgca
     1321 gggagacgtc gggtcgcgcc gtggcctctc cgaaggggta cagcgtgata gccacaaggt
     1381 cgatcggcag gatgccatgc tccgccatct ggcgttcgtg ctcgggcttc ccccgtatcc
     1441 ccaggagccc tccatggatt ctggggtgca gggtcttcac ccggccatcc agcatctcag
     1501 ggaactctgt aatttccgtc acgtcggtga cgggcagacc attctctcgc atgacgcggg
     1561 ccgtcccccc ggtcgagacg agctggacac cgagtctcat caggcccgcg gcgaaatcga
     1621 ccaggcccac tttgttggac gcgctcagaa gagcgcggcg cacgcgcatc atcccgttac
     1681 ctcgtcggcg aggaccgtgc ttggcccctc cggttccgtc tctccacggt cctggatcgc
     1741 cacgggtcgc cttgcaggac ccggccagcc ctccggacgg caaccggggg aggatcagac
     1801 gcatcgcctg cgtgcaggcg gacgggccat cgagccgtcc gatgagcagc cgaacgcggt
     1861 ctggacatga acgactg
//
LOCUS       DBNGZC010000721         1857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_648909, whole genome shotgun sequence.
ACCESSION   DBNGZC010000721 DBNGZC010000000
VERSION     DBNGZC010000721.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_648909"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1319)
                     /locus_tag="AC16G0_12425"
     CDS             complement(<1..1319)
                     /locus_tag="AC16G0_12425"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HIR5100453.1"
                     /translation="MATAFTQASYIAAAALFILSLKWLSHPDTARRGVRTGEVGMALA
                     IAGTLVQQGIVSYQWILVGFVLGTAIGLPMGVWMPMTHVPQRTALSHAFGALAAALVG
                     TSEYYLHTVELSTFTMAVLSLEVVLGFLTFTGSLMAVGKLQDLVPSRPLVYKGQNVVN
                     LLLLAVAVGTAVYLVMYPGRTELFPVVAGLSLLFGVLLIIPIGGADMPTVISLLNSYA
                     GLSAAAMGFALDNNLLIVAGALDGSSGFILSVIMCRAMNRSFTNVLFGGFGQVQVTAG
                     TAEQKSYRSATAEDAAGILEAARAVIIVPGYGMAVAQAQHKVRELYDILSRRGVEVKF
                     AIHPVAGRMPGHMNVLLAEADIPYDRLVEMDEINPEFAQADVALVIGANDVTNPAARH
                     DKASPIYGMPILDVDKARTVMVIKRSMSPGFAGIDNELYYMDRTMML"
     gene            complement(1319..1612)
                     /locus_tag="AC16G0_12430"
     CDS             complement(1319..1612)
                     /locus_tag="AC16G0_12430"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha part 2"
                     /protein_id="HIR5100454.1"
                     /translation="MPSNLESALFVFLLATFVGFEVIRRVSPLLHTPLMSLTNAISAI
                     SLVGSIAIAGAEKSHLSTVLGTIAVVCSMANVIGGFVITDRMLKMFKKREPGR"
BASE COUNT          327 a          623 c          607 g          300 t
ORIGIN      
        1 aagagcatca tggtcctgtc catgtagtag agctcgttgt cgatgccggc gaagcccgga
       61 ctcatgctgc gcttgatcac catgaccgtc cgagccttgt ccacgtccag gatgggcata
      121 ccgtagatcg ggctcgcctt gtcgtgccgg gccgcggggt ttgtcacgtc gttggcgccg
      181 atcaccaggg ccacgtcggc ctgcgcgaac tcggggttga tctcgtccat ctccaccagc
      241 cggtcgtagg gaatgtccgc ctcggccagg agcacgttca tgtggccggg catgcggccc
      301 gccacggggt ggatggcgaa cttcacctcc accccgcgcc ggctgaggat atcgtacagc
      361 tcccgaacct tgtgctgcgc ctgcgccacg gccatgccgt agccgggaac gatgatgacg
      421 gcgcgcgccg cttccaggat tcccgcagca tcctcggccg tggcgctccg atagctcttc
      481 tgctcggccg tgcccgctgt cacctggacc tggccgaagc ccccgaagag cacgttggtg
      541 aaggatcggt tcatggcccg gcacatgatt accgagagga tgaagcctga ggagccatcc
      601 agggcgccgg ccacgatcag caggttgttg tcgagggcga agcccatggc cgcagccgag
      661 aggcccgcgt aggagttgag gagcgagatg accgtcggca tgtccgcgcc gccgatgggg
      721 atgatgagga gaaccccgaa gagcagggac agcccagcga ccaccgggaa cagctcggtc
      781 cgaccggggt acatcaccag gtagaccgca gtccccaccg ccaccgccaa gagcaacaag
      841 ttgacgacgt tctggccttt gtacaccagc gggcggctcg ggaccagatc ctgcagcttg
      901 ccgactgcca tcaggctgcc cgtgaatgtc aagaagccca ggaccacctc caggctgagg
      961 accgccatgg tgaacgtgga cagctccacg gtgtgcagat agtactcgga cgtccccacc
     1021 agggccgccg ccagcgcccc gaaggcgtgg gacagcgcgg tccgctgcgg gacgtgggtc
     1081 atcggcatcc acacacccat gggcaatccg atggccgtcc ccaggacgaa gcccaccagg
     1141 atccactggt agctcacgat cccttgctgg acgagggtcc ccgcaatcgc cagggccatg
     1201 ccgacttcgc ccgtgcgcac gccgcgccgg gccgtgtcgg ggtggctcag ccacttgagc
     1261 gagaggatga acagggccgc ggcggcgatg taggaggcct gggtgaatgc ggtggcgatc
     1321 accgcccggg ctcccgcttc ttgaacatct tgagcatgcg gtccgtgatc acgaacccgc
     1381 cgatcacgtt ggccatggag cagacgacgg cgatggtccc caggaccgtg ctgaggtggc
     1441 tcttctccgc gccagcgatg gcgatggagc ccaccaggga gatggccgag atggcgttgg
     1501 tgagcgacat cagcggcgtg tgcaacagcg ggctcacccg gcggatcacc tcgaagccca
     1561 cgaaggtggc cagcaggaag acgaacagcg cgctctccag gtttgacggc atcccatgcg
     1621 cctcctctgt atgcggcttt ccttcaacgc tcgacggtac cgttgcaagc ctacgagccc
     1681 cttcccccca ggtgcgtctt ccgtcagtct tgcgcctggg tgatcggggt ctcgagatac
     1741 ggcagcccca gcgccttgcg cacccccgcg tgcaccacct caccaccgcg ggtcaagagc
     1801 gtcgcctgcg cgatctcgtc acccggtgtc gcctggaagg cgcctgtctg caggagg
//
LOCUS       DBNGZC010000722         3154 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1724476, whole genome shotgun sequence.
ACCESSION   DBNGZC010000722 DBNGZC010000000
VERSION     DBNGZC010000722.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1724476"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="AC16G0_12435"
     CDS             <1..583
                     /locus_tag="AC16G0_12435"
                     /inference="COORDINATES: protein motif:HMM:NF039835.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIR5100455.1"
                     /translation="NVTATYTLQIHALTVTVVGSSDGSVSGSSGGISCTPTCEASYTS
                     STVVTLTASAGAGGIFREWRGDCTGTGACQVTMTAAKSVTAVFSKTFTDGTPTAGVTP
                     IKAAHFTELRDAINTLRARYGLAAFAWSTPAPTAGGTVLKAHLTDLRTALTQAYSEAK
                     KTPPTYTEAISARETAIKASHLSELRNFVRALE"
     gene            843..1709
                     /gene="ypfJ"
                     /locus_tag="AC16G0_12440"
     CDS             843..1709
                     /gene="ypfJ"
                     /locus_tag="AC16G0_12440"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_02809 family neutral zinc metallopeptidase"
                     /protein_id="HIR5100456.1"
                     /translation="MRLDDERESDNVEDRRGEGGRGGFGVGRGVGIGTVIVALAASYF
                     LGIDPSVMLGLFSGGGTPVPQSVPARKPPAGDEMARFVSKVLADTEDTWREIFRQTGR
                     QYQDPRLVLFTGATPTACGTGQTAMGPFYCPEDQKVYIDLAFYQDLKARYKAPGDFAQ
                     AYVIAHEVGHHVQHLLGISDKVQAARRRASETQANALSVRLELQADCLAGVWGYHADR
                     VRNILETGDIEEALTAASAIGDDRLQQQGRGYAVPDTFTHGTSAQRVRWFKRGVESGD
                     LRQCNTFEARQL"
     gene            2105..2860
                     /locus_tag="AC16G0_12445"
     CDS             2105..2860
                     /locus_tag="AC16G0_12445"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HIR5100457.1"
                     /translation="MRAILLVAGAGWRLKPHTEATPKCLLDVGGKSLLLRYLEIFSAL
                     GIREAVLVVGFLKEMVMAEAARGPADMQVRFVQNDQFTKGNILSLWHARQECDDEVII
                     MDGDVLYPQELLARLLASPDPNAIVVDERFQDSGEEQKVVCEDGWVVEVTKKISGDPR
                     VRGEAVGFLRLSAEAAEVLRGILEEFIETGKDTLEYEETFRELAAEVPIGLVEVGDLP
                     WIEIDYEEDLARAREQILPQVECLDRGQIIPSA"
     gene            complement(2948..>3154)
                     /locus_tag="AC16G0_12450"
     CDS             complement(2948..>3154)
                     /locus_tag="AC16G0_12450"
                     /inference="COORDINATES: protein motif:HMM:NF019998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HIR5100458.1"
                     /translation="TVEPNRLPGTIERAVYTGSLVRYTVNVRGTRLAVDLSDPRHTPA
                     FVAGDRVTVILPDDPHLLSETDHD"
BASE COUNT          569 a         1008 c         1089 g          488 t
ORIGIN      
        1 aaacgtgacc gcgacctata ccctacagat ccatgcgctc acggtcacgg tcgtggggag
       61 cagcgatggc agcgtgagcg gctcctccgg cgggatatcc tgcaccccga cctgcgaagc
      121 gagctacacg agcagcacgg tggtcacgct cacggcgagc gcaggagcgg gcggtatctt
      181 ccgggaatgg cgcggggact gcaccggaac cggggcgtgc caggtcacga tgacggcggc
      241 gaaatcggtc acggccgtct tctccaagac gttcaccgat ggcacgccca cggcgggggt
      301 cacgcccatc aaggcggcgc acttcaccga gctgcgggac gccatcaaca ccctccgcgc
      361 ccggtatggc ctcgcggcct tcgcctggag cacgcccgcc cctactgccg gaggcacggt
      421 gctgaaggcg cacctgaccg accttcggac ggccctcacc caggcctact cggaggcgaa
      481 gaagacgccc cccacctaca cggaggctat ctcggccagg gagacggcca tcaaggccag
      541 ccacctgagc gagctgcgaa acttcgtgcg ggcgctagag tgaggtccta cccgagcccc
      601 gctgccattg caggcgaggt cgagtgagca cctccgtgta gtgtgggtga accggggccg
      661 aagggaggcg aaaagagcca aaggatgacc cgccaagggc gcggagtttt gtgttgctct
      721 cctgccaacg gcgaggtaag gtcactcact gagcgggtta cggtttggac cgaaagcgct
      781 gggaagatcg gttcggtccc cgtgctggga gtgatcacag acgggaactg gaggacactc
      841 gcatgcgcct cgatgacgaa cgggagagcg acaatgtcga ggaccgccgc ggcgagggcg
      901 gtcggggagg cttcggcgtc ggccggggcg tcggcatcgg cacggtcatc gtcgcgctgg
      961 cggcgagcta cttcctcggc atcgacccgt cggtgatgct gggtcttttc tcggggggcg
     1021 ggactcccgt cccgcagtcg gtgcccgccc gcaagcctcc ggccggcgac gagatggcac
     1081 gattcgtatc gaaggtgctg gcggacaccg aggatacctg gcgcgagatc ttccggcaaa
     1141 ccggccgcca gtaccaggac ccgaggctcg tcctgttcac gggcgcgacg ccgaccgcat
     1201 gcggcaccgg ccagacggcg atgggcccgt tctattgccc ggaggaccag aaggtctata
     1261 tcgatctcgc cttctaccag gacctgaagg ctcgctacaa ggcgcccggc gatttcgccc
     1321 aggcgtacgt catcgcgcac gaggtcggcc atcacgtgca gcacctgctc ggcatctcgg
     1381 acaaagtgca ggcggcccga cgccgtgcca gcgaaacgca ggccaatgcc ctgtcggtgc
     1441 gcctggagct ccaggccgat tgcctggccg gcgtctgggg ctaccatgcc gaccgagttc
     1501 gcaacatcct tgagaccggc gacatcgagg aagcgctgac ggccgccagc gcgatcggtg
     1561 acgatcgtct gcagcagcaa ggacgcggct acgcggttcc cgacacgttc acccacggca
     1621 cctcggcgca gcgcgtgcgc tggttcaaac gcggcgtcga gagcggcgac ctccggcagt
     1681 gcaatacctt cgaggcgcgg cagttgtgac caggccggcg tcaagggtgg ggcaaggcca
     1741 tcttctcgcg gaagcccgca ggaaacgcac tccagggatg tgagcgccga tcaaccaccg
     1801 aagtcggacg attccatcga cagaagggcc cggggctttg acaatcgatc ggccccgaga
     1861 aaccgagcgg ctgggggtga cctcggcgtc ggcggccttg ccttccaggt ttgccgagtt
     1921 catggccatc ggaaggacga tcggggtgca tcgcagcgcc ggatcggcgc gcaagcaccc
     1981 acagacctcg aagccaacca tgccgggcat aagaatgtcc agcgggattc tccttgacac
     2041 cccgctttgc cctcgcatag catcgccgca gtttgcctga ccctcgcctt tccggggata
     2101 gagcatgcgt gccattctgc tggttgccgg agcgggctgg cggctgaagc cgcacaccga
     2161 ggcgaccccg aagtgtctcc tggacgtggg gggcaagtcc ctgctgctcc gctacctgga
     2221 gatcttctcg gccctgggga ttcgcgaggc tgttctggtg gtgggtttcc tgaaagagat
     2281 ggtcatggcc gaggctgccc gcggtccggc agacatgcag gtgcgcttcg tgcagaacga
     2341 ccaattcaca aaggggaaca tcctctccct ctggcacgcc cggcaggagt gcgacgacga
     2401 ggtcatcatc atggacggcg acgtgctcta tccgcaagag ctcctggcgc gcctcttggc
     2461 ctcccccgat cccaatgcca tcgtcgtgga cgagcggttc caggacagcg gcgaggaaca
     2521 gaaggtggtc tgcgaggacg gctgggtggt ggaggtgacc aagaagatca gcggtgatcc
     2581 gcgagtccgg ggcgaggccg tggggttcct tcggctctcc gccgaggccg ccgaggtcct
     2641 gcggggcatc ctggaggagt tcatcgagac cggcaaggac accctggagt acgaggagac
     2701 cttccgggag ctcgccgccg aggtgccgat cggcctggtc gaggtcgggg atcttccctg
     2761 gatcgagatc gactacgagg aagacctcgc ccgggcgagg gagcagatcc tgcctcaggt
     2821 ggagtgcctg gatcgcggcc agatcattcc ctccgcataa gcccaattct cacaccgcaa
     2881 ggctcgcagg gacggacgac ggcgcaggtc gggcggatga cccggcgggg cgggaggcgc
     2941 gcttcggtca gtcgtggtcc gtctcggata ggaggtgggg atcgtcgggc agaatcacgg
     3001 tcacccgatc cccagcgaca aaggcggggg tgtggcgcgg gtcggaaaga tccaccgcca
     3061 gccgggtccc ccttacgttc accgtgtagc ggacaagaga tccggtgtag acggccctct
     3121 cgatcgtgcc cggcaaccga ttgggctcca cagt
//
LOCUS       DBNGZC010000723         2284 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_944060, whole genome shotgun sequence.
ACCESSION   DBNGZC010000723 DBNGZC010000000
VERSION     DBNGZC010000723.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_944060"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="AC16G0_12455"
     CDS             complement(<1..375)
                     /locus_tag="AC16G0_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HIR5100459.1"
                     /translation="MKRYLLAPGPTPIPPEVLQVMAQPIIHHRTPEYEGLFGDVRRDL
                     RLLFQCKNEVLMFAASGTGAMEGAVVNTLSSGDRAVVVRGGKFGERWCEICQAYGVRV
                     LPLDVPYGKSVDPAAVAAALAQA"
     gene            688..1569
                     /locus_tag="AC16G0_12460"
     CDS             688..1569
                     /locus_tag="AC16G0_12460"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR5100460.1"
                     /translation="MTEDGRLRGYAMVVGAACCWGVMATLAKLLFRDRAVDPVVLVVI
                     RSDLATLTLFAIFAVAGLGRLRIGRREGWLAAIVGVGGLLTNNFLYFQAIHLTSVATA
                     LLLQYQAPILVALYTVLVQRQRLSRRLLLSLGMAAGGCALVVRVYDPAVLRPNLLGVA
                     AGLGTALAFAFYILASRVALKSMGAWTLLAYGYLSASLVWAFVVPPWRILSLPYDAET
                     WWAFVAIATIGTALPFGLFINGLKFLPPTQASIVSMLEPVVATLAAYIVLGETLLPLQ
                     MLGGGLVLAAVILVEKT"
     gene            1818..>2284
                     /locus_tag="AC16G0_12465"
     CDS             1818..>2284
                     /locus_tag="AC16G0_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR5100461.1"
                     /translation="MDYKETLNLPKTDFPMRANLAQREPELLRQWDRDRVYARLREVR
                     AGREMWILHDGPPYANGHIHMGHALNKILKDMVVKSRSMAGYDAVYVPGWDCHGLPIE
                     HQVDKELGAKAHEVPIPEKRRMCRAYAQKFIDIQREELKRLGVLGDWDHPYLT"
BASE COUNT          382 a          729 c          750 g          423 t
ORIGIN      
        1 cgcttgggcg agggctgcgg ccacggcggc ggggtccacg ctcttcccgt aggggacatc
       61 cagcggcagc acgcgaaccc cgtaggcctg gcagatctcg caccaccgct caccgaactt
      121 ccccccgcgg accaccacgg cccggtcacc ggaggagagg gtattcacca cggctccctc
      181 catggccccg gtgcctgagg cggcgaacat caagacctcg ttcttgcatt ggaacagcag
      241 gcggagatca cggcgcacgt cgccgaacag accttcgtac tcgggcgttc ggtggtggat
      301 gatgggctga gccatcacct ggaggacttc gggtggaatc ggagtcggac ctggcgcgag
      361 caggtatcgc ttcatcaatc atcccccctc tgtggatatg acgacgaaga gagcccggat
      421 catgaagtgc agtgctgagg aacccgacac ggggcagacc gggattccgt tcttgcagcg
      481 aaggacgcaa cccgtgggga ctatactcaa gcctcgcgag gcgtgtcaag gcgattcccc
      541 ggagcctcca gagggtcgcg gcccggctcg ggcgcttgcg aggtgcggtg gcgtatgcta
      601 gggtgccccg gcgatttgcc gaccgggaat ctcggaactc tggattgtcc gtttgccacg
      661 ttgcctgatt gccggcaagg ggagtagatg accgaggacg gccgcttgcg gggatacgcc
      721 atggtggtcg gggcggcctg ctgttggggc gtgatggcca cgctggcgaa gctcctcttc
      781 cgggacaggg ccgtcgatcc cgtggtcctg gtggtgatcc gctcggactt ggccaccctc
      841 accctctttg cgatcttcgc cgttgccggt ctcggacgat tgcggatcgg gcggcgggaa
      901 ggctggctgg cggccatcgt gggggtgggc ggtctcttga ccaacaactt cctctacttc
      961 caggccatcc atctgaccag cgtggcgacg gccctcctcc tccagtacca ggcacccatc
     1021 ctggtcgccc tgtacacggt cctggtccaa cgccagcgcc tgtcccgacg actcctacta
     1081 tctctcggaa tggcagcagg ggggtgcgcg ctggtcgtcc gggtgtatga tccggcggtg
     1141 cttcggccga acctcctcgg cgtggcggcg ggcctcggga cagccctcgc ctttgccttc
     1201 tacattctgg ccagccgcgt cgcgctgaag agcatgggcg cctggacgct cctggcctat
     1261 ggctaccttt ccgcaagcct ggtctgggca ttcgtggtgc cgccgtggcg gattctcagc
     1321 ctgccatacg acgctgaaac ctggtgggcc ttcgttgcca tcgcgaccat cggcacggcg
     1381 ttgcccttcg gtctgttcat caatggactg aagttccttc cacccaccca ggccagcatc
     1441 gtcagcatgc tggagccggt agtcgccacg ctggcggcct acatcgtcct gggggaaaca
     1501 ctgcttccac ttcagatgct ggggggaggg ctcgtcctgg ctgcggtcat cctggtcgag
     1561 aagacgtgag caggccgcgt tcatgagggc gcgggtgacg atcacatcgg tcttccccaa
     1621 cccctcattg agggacaggg cccatcggtt gacaggggac tgcttccctg gtagctttta
     1681 gcctccaggc gggtctcagc gtgcgaaaat gaacgccccg gagtggggcg tttttcattc
     1741 gcacccagcc atcagccccc ggcgatcggc ctcgatccgg cggatgatgg cttccttctg
     1801 ccaagggccg tgaggacatg gactacaaag agacgctgaa cctccccaag accgacttcc
     1861 ccatgcgagc caacttggcg cagcgagagc ctgagttgct ccgccagtgg gaccgggatc
     1921 gcgtgtatgc ccgcctgcgc gaggtgcgag ccgggcggga gatgtggatc ctgcatgacg
     1981 ggccgcccta cgccaacggt cacatccaca tgggccacgc gctgaacaag atcctcaagg
     2041 acatggtggt gaagtcccgg tccatggcgg gctacgatgc ggtgtacgtg ccgggctggg
     2101 actgccacgg cctccccatc gagcaccagg tggacaagga gctgggagcc aaagcgcacg
     2161 aggttcccat cccggagaag cgccggatgt gccgggccta tgcccaaaaa ttcatcgaca
     2221 ttcagcgaga ggagttaaag cgcctgggtg tcctggggga ttgggaccat ccctacctca
     2281 ccat
//
LOCUS       DBNGZC010000724         1504 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_551062, whole genome shotgun sequence.
ACCESSION   DBNGZC010000724 DBNGZC010000000
VERSION     DBNGZC010000724.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_551062"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="AC16G0_12470"
     CDS             <1..560
                     /locus_tag="AC16G0_12470"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIR5100462.1"
                     /translation="PEYLLLGKTMDRRGNRDRYCAPSNLFRAKGGEWVYVAAANDSLF
                     PQLLKVMGHEEVLQDPRFATNDARMAHVEEAEEVLAKWVAGRTADEVVALVRGAGLPC
                     AKIASIEDVVTNPQVRHRGMLVELEHPTAGRVPMHGLNVHFSNTPKQIRRPAPLLGQH
                     NEEVYGRWLGFTPEHVSQLRSEGVI"
     gene            787..>1504
                     /locus_tag="AC16G0_12475"
     CDS             787..>1504
                     /locus_tag="AC16G0_12475"
                     /inference="COORDINATES: protein motif:HMM:NF013323.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR5100463.1"
                     /translation="MQRKLIGLSEAARLVSSGSSIALGGSIIRRSPAAFARELIRQGV
                     TDLRILAFQAWFTMDLLAGAGAVKRVEAVYEGLFQFGMAFNFRRAVEAGKIEVRDFPE
                     TAMIARFRAAAQGVSFLPTQVLLGTDMAAHNPEQIREITCPFTGGKYHAVEAAHTDFT
                     VLHGYVGDEFGNVQYPVQRDSDDIDSIMAKAANRLIVTVEKIVPHEEILRRANLTYIP
                     HVWVEAIVEAPFGAHPLSCDG"
BASE COUNT          276 a          441 c          539 g          248 t
ORIGIN      
        1 tccccgagta tctgctctta gggaagacga tggaccgacg gggcaaccgt gaccggtact
       61 gtgccccctc caatctgttc cgcgcgaagg gtggcgagtg ggtgtacgtg gccgcagcca
      121 acgactccct gttcccgcag ttactgaagg tgatggggca tgaggaggtc ctgcaagacc
      181 cgcggtttgc gacgaatgac gcccgcatgg cccatgtgga ggaggcggag gaggtgctgg
      241 ccaagtgggt ggcgggtagg acggcggacg aggtggttgc gctggtgcgg ggggccggtc
      301 tgccgtgcgc gaagatcgcc tccatcgaag acgtggtgac gaatccccaa gtgcggcatc
      361 gcggaatgct ggtcgagctg gagcatccca ccgccggacg cgtgcccatg cacggcctga
      421 atgttcattt ttcgaacacc cccaagcaga ttcgacgccc cgccccgttg ctcgggcagc
      481 ataacgagga ggtgtatggg cggtggctgg ggttcacacc cgagcacgtc tctcaactga
      541 ggagcgaagg ggtcatctga ggtagccgag aaacggcgca acactcccgg ggcgaaggag
      601 actcgtgtgg atggaccgag catgcggagg aggtgatgag gcgaacacac tctcccagga
      661 aagacccctt ggatggtcgg gtgcgtacga agggccgcag gccggaaagc caagccggcg
      721 ggtatgggga gcaccgcgag cgacggggtc ccgacgaggc acactaattg tgggaggcgg
      781 agggctatgc agcgcaaact catcgggctg tccgaggcgg ccaggttagt ttcctcggga
      841 agcagcattg cccttggcgg cagcatcatt cggcgatcgc cggcagcctt tgcccgcgag
      901 ctcattcggc agggcgtgac ggatctccgc attctggcct tccaggcgtg gttcacgatg
      961 gacctgctgg ccggggcggg agccgtcaag cgagtcgagg ccgtctacga ggggctcttt
     1021 cagttcggga tggccttcaa cttccgacgg gccgtcgagg cggggaagat cgaggtccgg
     1081 gacttccccg agacggccat gatcgcgcgg tttcgggcgg cggcgcaggg ggtctccttc
     1141 ctgccgacgc aggtgctcct ggggacggac atggcggccc ataacccgga gcagatccgc
     1201 gagattacct gcccgttcac cggcgggaaa taccacgccg tcgaggcggc ccacaccgac
     1261 tttaccgtgc ttcacgggta cgtgggagat gagttcggaa atgtccagta cccggtgcag
     1321 cgcgacagcg atgacatcga ctcgatcatg gcgaaggcgg ccaaccgact gatcgtcacc
     1381 gtcgagaaga tcgtccccca cgaggagatc ctgcgccggg cgaacctgac gtacatcccc
     1441 cacgtgtggg tggaggccat cgtcgaggcc ccgttcggtg cgcacccgct gagctgcgat
     1501 ggct
//
LOCUS       DBNGZC010000725         1917 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_649238, whole genome shotgun sequence.
ACCESSION   DBNGZC010000725 DBNGZC010000000
VERSION     DBNGZC010000725.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_649238"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..633)
                     /locus_tag="AC16G0_12480"
     CDS             complement(181..633)
                     /locus_tag="AC16G0_12480"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR5100464.1"
                     /translation="MQPLERDVETLRNIPLFAGLPTARLKLIAYTAEVVRFEPGEEIV
                     QQGDPADAVYIIAEGEAEVWLTAVDGHEVRLGTMGRHSLLGEIAVLCNGRRTATVRAK
                     DPMATLKISAQVFLDLVRQSPDISMKVMTILAERLEKTTALLRQSDHQ"
     gene            complement(647..>1917)
                     /locus_tag="AC16G0_12485"
     CDS             complement(647..>1917)
                     /locus_tag="AC16G0_12485"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5100465.1"
                     /translation="PQGPGGEPDGASSDGVRADEPGSWLDLSLAGVNDRAGLVAAVLA
                     STRLVALDEDLFTFGLRACIDPAKRPEITERLLVARRLVMERFGQEGKGVVVEFFDRD
                     RFAAYASIGENILFGQSTDPELGMDRLAAHDHFRRVIAEVGLKDSLLTLGAEVAREMV
                     EIFKDIPAEHELFSNFSLIAASELPEYVRVLSRLERITPQALPPEDQERLIALALRLI
                     PARHRLGHVDEPFMAKVVAARHRFAESLPPSVGTFVPYDRQRYFAQGTLLENLLFGKV
                     LATSSLAVKKVNAIVEEVITTHNLREVVMEAGLDYPVGMAGSRVSPVQRQKVVVARAL
                     LKRPQILILDDAFSALESDKLAVMHQRITEAMKGRTVISVVKHPDLVRYYDSVVVLDA
                     GKVAEMGSYQELAAKDGIFRHLLVKASAST"
BASE COUNT          318 a          650 c          611 g          338 t
ORIGIN      
        1 ggcgggccga tcggggtgga tcccccaagc tccgcgcctg ttctcgcaac ttctccacca
       61 tggcggaacc cctggcgaaa ctcgacggcg tctcctgcgt caccgcgcgg aagtgcgaac
      121 cccgctcccc ctccggagat tctttccggg ggactccgcg tggacaaccc ccggccttgg
      181 ttactggtga tcggactgcc ggaggagtgc cgtggtcttc tcgagccgct ccgcgaggat
      241 cgtcatcacc ttcatgctga tgtccggact ctggcgcacc aggtccagga acacttgggc
      301 cgagatcttg agggtcgcca tgggatcctt ggcacgcacc gtggccgtgc gccggccatt
      361 acacaagacc gcgatctccc ccagcaggct gtggcggccc atggtcccca gacgtacctc
      421 gtggccgtcg acggccgtga gccagacctc cgcctcgccc tcggcgatga tgtagacggc
      481 gtccgcggga tccccctgct gcacaatctc ctctcccggc tcgaaccgca ccacctccgc
      541 agtgtaggcg atcagcttga gcctggctgt gggcagaccg gcaaacaggg ggatgtttcg
      601 cagcgtctcg acgtcccgct ccaggggttg catctcggct tctccttcag gtggaggcgc
      661 tggccttgac caggaggtgg cggaagatac catccttggc cgccaattct tgataagagc
      721 ccatctcggc gaccttgccc gcgtcgagca ctacgacgga gtcgtagtag cggaccaggt
      781 cggggtgctt gaccaccgag atgaccgtgc gtcccttcat ggcctcggtg atgcgctggt
      841 gcatgacggc cagcttgtcc gactcgaggg cactgaaggc gtcgtccagg atgaggatct
      901 gcggacgttt gagcagcgcc cgcgccacca cgaccttctg gcgctgtacc ggggacactc
      961 ggctgccggc cattcccacg gggtagtcga gacccgcctc catcaccacc tcgcggaggt
     1021 tgtgggtggt gatcacttcc tccacaatgg cgttgacctt ttttacggcc aggctgctgg
     1081 tggcgagcac tttgccgaac agcaggttct ccagcaaggt gccctgggcg aagtaccgct
     1141 ggcgatcata gggcacgaaa gtccccacac tgggtggcag gctctcggca aagcgatgcc
     1201 gcgcggccac gaccttggcc atgaacggct cgtccacgtg acccaaccgg tgccgggccg
     1261 ggatcaaacg tagggcaagg gcgatcagcc gctcctgatc ctcgggggga agcgcttgag
     1321 gggtgatccg ctccaaccga gacaggaccc gcacgtattc gggcaactcg ctggcagcga
     1381 tcaggctgaa gttcgagaag agttcgtgtt ccgctgggat gtccttgaag atctccacca
     1441 tctccctggc gacctcggcg cccagtgtca ggagggaatc cttgaggccc acctcggcaa
     1501 tgacccggcg gaagtggtcg tgggcggcga gccggtccat gccgagttct gggtccgtgc
     1561 tctgcccgaa caggatgttc tccccgatcg aggcataggc ggcgaaccga tcccggtcga
     1621 agaactccac caccaccccc tttccttctt gcccgaaccg ctccatcacg aggcgtcggg
     1681 cgacgaggag gcgttcggtg atctcgggcc gcttcgccgg atcgatgcag gcccgcaggc
     1741 cgaaggtgaa gagatcctcg tccagcgcca ccaagcgtgt ggaggcgagc acggcggcca
     1801 ccagaccggc cctatcgttc acgcccgcca gcgacaagtc cagccaactg ccgggctcat
     1861 ccgcccttac gccgtccgag ctggcgccgt caggctcgcc gccggggccc tgggggc
//
LOCUS       DBNGZC010000726         1186 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_359269, whole genome shotgun sequence.
ACCESSION   DBNGZC010000726 DBNGZC010000000
VERSION     DBNGZC010000726.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_359269"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="AC16G0_12490"
     CDS             <1..728
                     /locus_tag="AC16G0_12490"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR5100466.1"
                     /translation="INTAIFSQTGGSVGIGFAIPINEAKSILPELQAKGHVTRGWLGV
                     SIQPVTPELAKALHLEKDKGALVAQVMPNSPAAKAGLKEGDVIVEYEGHAVTKSSDLP
                     RLVASTPIGKAASLQVLRDNQPLAFSAQIAELSEPQTAATSNPDREKLGIAVQSLTPA
                     LAQQLGVPDRSGVVVAGVKDGSPAAEAGVQPGDVIVQANRQPVKNVTDFRKALAAQKP
                     DEPTLLQIHRKDASLFVAVTAQG"
     gene            750..>1186
                     /locus_tag="AC16G0_12495"
     CDS             750..>1186
                     /locus_tag="AC16G0_12495"
                     /inference="COORDINATES: protein motif:HMM:NF012436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR5100467.1"
                     /translation="MHHARWLPPSAVAAGPVTVFEGNLMDDRELVEGLNRDLAQELGT
                     ICRYIQQAALARGRSGHDLRVFLKVEVIDDVFHAMFLADKVAQLGGTPTTEPAPFKLL
                     RNPKEMIEYDLEIERRAIHHYAERVKQAAADWALSLKARLEKIL"
BASE COUNT          236 a          377 c          386 g          187 t
ORIGIN      
        1 gcatcaatac cgcgatcttt tcccaaaccg ggggctccgt ggggattgga tttgccatcc
       61 ctatcaatga ggccaagagc attctacccg agctgcaggc caagggacac gtgacccgag
      121 gatggctcgg cgtgagcatc cagccggtga cccccgagct ggccaaggcc ctccacctgg
      181 agaaggacaa aggggccctg gtggcacagg tcatgccgaa ttcccccgct gccaaagccg
      241 gtctgaagga gggcgatgtg atcgtcgagt atgaggggca tgccgtgacc aagtccagcg
      301 acctgccccg tctggtcgca tcaacgccga tcgggaaggc tgcctctctc caggtgctcc
      361 gggacaacca acccctcgcc ttcagcgcac agatcgcgga gctctccgaa cctcagacgg
      421 cggccacgtc caacccggac cgggagaagc tgggtattgc ggtccagtct ctcacgccgg
      481 cgctggccca acaactggga gtgcccgacc gctccggggt cgtggtcgcc ggcgtgaagg
      541 acggtagccc ggcggcggag gcgggagtcc agcccgggga tgtcatcgtt caggccaacc
      601 ggcaaccggt gaaaaacgtc accgattttc gcaaagctct ggctgcccag aagcccgacg
      661 agccgacgct cctccagatc caccggaagg atgccagcct atttgtggca gtgacggccc
      721 agggctagat ccagcgctgg ggtgacggcg tgcatcacgc gcgctggctg cccccgagcg
      781 ccgtggccgc gggtccggtg acggtattcg aggggaatct catggatgat cgagagttgg
      841 ttgaaggact caaccgggac ctggcgcagg aactcgggac gatctgccgg tacatccagc
      901 aggcggccct ggcccgagga cgctctggcc acgacctccg ggtattcctg aaggtggagg
      961 tcattgacga cgtgttccat gccatgttcc tggcggacaa ggtcgcgcaa ctgggcggca
     1021 cacccacgac agagcctgcc cctttcaaac tcctccggaa cccgaaggaa atgatcgagt
     1081 acgacctgga gatcgagcgc cgcgccatcc atcactatgc agagcgagtg aagcaggctg
     1141 cggcggattg ggcgctcagc ctcaaggcgc gactggagaa gatcct
//
LOCUS       DBNGZC010000727         2376 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1134458, whole genome shotgun sequence.
ACCESSION   DBNGZC010000727 DBNGZC010000000
VERSION     DBNGZC010000727.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1134458"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="AC16G0_12500"
     CDS             complement(<1..632)
                     /locus_tag="AC16G0_12500"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR5100468.1"
                     /translation="MRLALDVGNTNTVIGVFDGAALRVHWRLSTRRTGTSDEYGIFTK
                     GLFDFAGLDFRAVTAVILSTVVPTVQAPLEEMSRQFFGVEPMVVGPGIKTAMPILYES
                     PRDVGADRIVNAVAAFEAYGGPCIVVDFGTATTFDAISVKGEYLGGVICPGIGISAEA
                     LFQRAAKLPRVDIVKPKVVIGKNTVGSMQAGLFYGYLSLVEGIVARMREE"
     gene            complement(769..936)
                     /locus_tag="AC16G0_12505"
     CDS             complement(769..936)
                     /locus_tag="AC16G0_12505"
                     /inference="COORDINATES: protein motif:HMM:NF014310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100469.1"
                     /translation="MRSATLGNPVHVLAGSEDWQGQAVDLDPDGALLVRDESGATRRV
                     VAGDVSIQSPG"
     gene            complement(959..1546)
                     /locus_tag="AC16G0_12510"
     CDS             complement(959..1546)
                     /locus_tag="AC16G0_12510"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR5100470.1"
                     /translation="MGTIPADIAPEAIRGTLLAPVIGRVLEVLPEIGSTNDRAMAAGD
                     GGTAEGFCVLADRQTAGRGRLGRTWVSPPGVGLYTSILLRPKAPLSSLPLLTLVAGLA
                     VTDAIREVAALTARLKWPNDVWLDGRKVAGILTELATSASSVRYVVVGIGINVNHSAD
                     DFPAELRSADRPRVAGRCHLQRYGQLVRPFLPGPA"
     gene            complement(1620..>2376)
                     /locus_tag="AC16G0_12515"
     CDS             complement(1620..>2376)
                     /locus_tag="AC16G0_12515"
                     /inference="COORDINATES: protein motif:HMM:NF019869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR5100471.1"
                     /translation="ASILYQFLWHEYCDWYLEIVKDRLTTPASEADARAGRILLVHVL
                     EQSLRLLHPIMPFITEEIWQRLPHQGLSIMLAPWPQPDPALDWPKAVESMDLLMEITR
                     EVRNIRSTYNIPPAKRLPLVLHTGSAAQDAILESCRGYLTNLARLSRLTFGQGVAKPD
                     VAATAVVKGIEAHVPLEDVIDLREEQARLARELAKVEKALERITKKLSNEEFLGKAPA
                     AVVSQERAVNAELLDAQVKIRESAARIAAHLKG"
BASE COUNT          411 a          808 c          743 g          414 t
ORIGIN      
        1 atctcctccc gcatccgagc cacgatcccc tccaccaggc tcagataccc atagaagagg
       61 ccggcctgca tgctgcccac ggtgttcttg ccgatgacga ccttgggctt gacgatgtcc
      121 acgcggggga gtttggccgc ccgctggaag agggcttccg cggagatgcc gatgccggga
      181 cagatgacac cgcccaggta ctcgcccttg accgagatag cgtcgaaggt ggtggccgtg
      241 ccgaaatcca cgacgatgca ggggccgcca taagcctcga aggccgccac ggcattcacg
      301 attctgtcgg cgcccacgtc ccggggcgat tcgtacagga tgggcatggc ggtcttgatc
      361 cccggcccca ccaccatcgg ttccacgccg aagaactgcc ggctcatctc ctccagcgga
      421 gcctggactg tcggtaccac ggtggagaga atgacggccg tcaccgctcg gaagtccagg
      481 cctgcgaaat cgaagagccc cttagtgaag atgccgtact catcgctcgt acccgtgcgt
      541 cgagtggaca ggcgccagtg gaccctgagc gctgcgccgt cgaagacgcc gatcaccgtg
      601 ttcgtgttcc cgacgtccag cgccagacgc atgtctcctc ctctccttgc tcgctctcca
      661 ggtgtggacg gaaagaggcc agagatcaga ggccagaggt cagatgatca gcgatcagag
      721 gtcagtggtc agcgctcggc ttgatgccga cggacgagcg ctgacttctc atcccggcga
      781 ttggatcgac acgtcccccg ccaccaccct gcgagttgct ccggattcgt ctcggaccag
      841 cagggccccg tccggatcca ggtccacggc ctgtccttgc caatcctcgc tgccggcgag
      901 cacatgtact gggttcccca gggtggcact tctcaaccga ccggcctcca ggatcatttc
      961 acgccggtcc tggcaaaaaa gggcgtacca gttgtccata ccgttgtaga tggcagcggc
     1021 cagcaacccg cgggcgatcg gccgaccgca gctccgcggg aaaatcgtct gcgctgtgat
     1081 tgacgttgat accgataccc acgacgacgt acctcactga tgacgcgctt gtcgccagct
     1141 ccgtcaggat accggccact ttgcgtccgt ccagccagac atcgttgggc cacttcaggc
     1201 gcgcggtcag cgcggccacc tcacggatcg cgtccgtcac tgccagaccg gccaccaggg
     1261 tgaggagtgg cagggaagat aacggcgctt tcggccgcag caggatcgag gtgtagagac
     1321 ccacgccagg cggcgagacc cacgtacgtc ccaggcgccc gcgacccgcc gtctggcgat
     1381 cggcgagaac gcaaaagccc tcagccgttc ctccatcgcc cgcagccatg gcccgatcgt
     1441 tggtcgagcc gatctctgga agcacctcta gcacgcggcc gatcacgggc gcaagaagcg
     1501 tcccccggat ggcctcggga gcaatgtccg ctggaatcgt acccattgct tgccactcgg
     1561 ccatcagccc ccagttttca gcgtggagct gatggctgac ggctggccgc tgtcctcggt
     1621 tatcccttga ggtgcgccgc gatgcgcgcc gcgctttccc ggatcttcac ctgggcatcc
     1681 aggagttccg cgttgacggc ccgctcttgg ctcacgacgg cggcgggcgc cttgcccagg
     1741 aactcctcgt tgctgagctt cttcgtgatc cgctccagcg ccttctccac cttcgccagt
     1801 tcccgggcga gacgtgcctg ctcctcgcgg aggtcaatca catcctccag ggggacgtgg
     1861 gcctcgatcc ccttgacgac ggcggtggcg gccacatcgg gcttggccac gccctgcccg
     1921 aaggtgagcc gcgagagccg cgccaggttg gtcagatagc cgcgacagga ctccaggata
     1981 gcgtcctggg ccgcgctccc ggtgtggagc accaggggca gccgcttggc cgggggaatg
     2041 ttgtaggtgg accggatgtt ccgtacctcg cgggtgatct ccatgaggag atccatgctc
     2101 tccaccgcct tcggccaatc cagcgcggga tccggctgcg gccagggcgc cagcatgata
     2161 ctcagcccct ggtgcggaag ccgctgccag atctcctcgg tgatgaaggg catgatcggg
     2221 tggagcaggc gcagagactg ctccaggaca tgcaccagga ggatccggcc cgcccgtgcg
     2281 tccgcctcgc tggccggagt cgtcagcctg tccttcacga tctccaggta ccagtcgcag
     2341 tactcgtgcc agaggaactg gtagaggatc gaggca
//
LOCUS       DBNGZC010000728         1719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2608029, whole genome shotgun sequence.
ACCESSION   DBNGZC010000728 DBNGZC010000000
VERSION     DBNGZC010000728.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2608029"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="AC16G0_12520"
     CDS             <1..558
                     /locus_tag="AC16G0_12520"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015589809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HIR5100472.1"
                     /translation="SSMNWAFSYAATGYIDYGMIGAAQVDTYGNVNSTLLGGSYERPG
                     RRFPGSGGGNELASLCWKTILVMKHEPRRFVKELDFITSPGYLDGPGRREEAGLPRGS
                     GPWRLVTSRAVFGFEAETKRLQLLGVLKGLTEREVLDGMEVRPLVADTLEVLAPPSEE
                     ELSVLRREIDPSGIIIRGEKLRAPR"
     gene            605..1420
                     /locus_tag="AC16G0_12525"
     CDS             605..1420
                     /locus_tag="AC16G0_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015948541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5100473.1"
                     /translation="MRQSPLEQICLKVDDLSIAFGGVQALAGVSLDVRRGEIFSIIGP
                     NGAGKTVLLNCINGLYRPSAGRILFEGQDITHLVPHRRAALGIARTFQKIELFSGMTV
                     LDNIRLGRHLHMRAGILSGACYLGKAAHEEVAHREFIEREIIDLLEIAHIRDRIVGML
                     PYGLQKRVELGRALALQPRILLLDEPMAGLNLEEVEDIARFILDLNEEDRWRVTCILV
                     EHDMGVVMYISHRVAALNFGEKIAEGPPGVVQGDAEVVRAYLGDVEGLYVSRR"
     gene            1404..>1719
                     /locus_tag="AC16G0_12530"
     CDS             1404..>1719
                     /locus_tag="AC16G0_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5100474.1"
                     /translation="MSRGGKLDELEITPDLTIPKLFVRKAGEHNGRVAIREKEYGIWR
                     PITWVQYLENVRVFALGLAALGLRPGDKVAMIGDNRPEGLWAEMGALSVGGVAVWLFQ
                     DAM"
BASE COUNT          299 a          492 c          627 g          301 t
ORIGIN      
        1 agcagcatga actgggcctt ctcctatgcc gccaccggct acatcgacta cggcatgatc
       61 ggggcagccc aggtggacac gtacggcaac gtgaattcca cgctgcttgg aggaagctac
      121 gagcgacccg ggcgacgatt cccgggcagc ggcggcggca acgagctggc ctccctctgc
      181 tggaagacga tcctggtgat gaagcacgag cctcgacgct tcgtgaagga gctggacttc
      241 atcacctcac ccggatacct ggacggtccg gggcggcggg aggaggcggg gctgccgcga
      301 ggcagcggac cctggcgact ggtgacgtcc cgggcggtgt tcggcttcga ggcggaaacg
      361 aagcgtctgc agcttctcgg ggttttgaag ggtctgaccg agagggaggt tctggatggg
      421 atggaggtcc ggccactcgt ggccgatacc ctcgaagtct tggccccgcc gagtgaggag
      481 gagttgtcgg tgttacgccg cgagatagac ccctcgggca tcatcatccg gggagagaag
      541 ctgcgcgccc cccgctaggt cggtcttcga tgttcgccgc gtagattgga gggggaaagc
      601 cggaatgcgt cagtcgccgc tggagcagat ctgcctcaag gtggacgatc tgtccatcgc
      661 gttcggaggc gtccaggccc tggccggggt gagtctggac gtgagaaggg gggagatctt
      721 ctccatcatc gggcccaacg gggcagggaa gaccgttctc ctcaattgca tcaatgggct
      781 gtaccggccg agcgccggcc ggatcctttt cgaaggacaa gacatcaccc acctggttcc
      841 gcatcggcgc gccgccctgg ggatcgcgcg caccttccag aagatcgagc tcttctccgg
      901 gatgaccgtg ttggacaaca tccggttggg acgtcacctg cacatgcgcg ccgggatcct
      961 ctcgggggcc tgctacctgg ggaaggcggc gcacgaggag gtggcccatc gggaattcat
     1021 cgagcgggag atcatcgacc tcctggagat cgcccacatc cgcgaccgga tcgtgggcat
     1081 gctcccctac ggtttgcaga agcgggtgga gctggggcgc gcgctggcgc ttcagccgcg
     1141 aatcctgctc ctggatgaac ccatggccgg gctgaacctg gaggaggtgg aggacatcgc
     1201 tcgcttcatc ctggacctga acgaagagga tcgctggcgg gtgacgtgca tcctggtgga
     1261 gcacgacatg ggggtcgtca tgtacatctc ccaccgggtg gcggcgctga attttggcga
     1321 gaagatcgcg gagggaccgc cgggagttgt gcagggcgat gcggaggttg tccgggcgta
     1381 cctgggggat gtcgagggct tgtatgtctc gcggcggtaa gctcgacgag ctggagatca
     1441 cacccgacct gacgatcccg aagttgtttg tgcggaaggc gggagaacac aacggccggg
     1501 tggccatccg cgagaaagag tacggcatct ggcggcccat cacctgggtg cagtacctgg
     1561 aaaacgtccg ggtgttcgct ctggggctcg cggccctggg tctccggccg ggagacaagg
     1621 tcgccatgat cggtgacaac cgccccgagg ggctctgggc agagatgggc gccctgtcgg
     1681 tcgggggcgt ggcggtgtgg ctgttccagg atgccatgc
//
LOCUS       DBNGZC010000729         1778 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2315373, whole genome shotgun sequence.
ACCESSION   DBNGZC010000729 DBNGZC010000000
VERSION     DBNGZC010000729.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2315373"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="AC16G0_12535"
     CDS             <1..614
                     /locus_tag="AC16G0_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIR5100475.1"
                     /translation="FVAVAGEVAGVVAVADTLKASSVEAIRALHVLGLEVVMITGDNQ
                     RTAAAIARQAGIDRVLAEVLPEQKASEVKKLQAEGKVVAMVGDGINDAPALAQADVGI
                     AIGTGTDVAMEASDITLIRGDLTGVVTAIELSQRTLRTIKQNLFWAFIYNVLGIPIAA
                     GALYPFFGILLDTMLASAAMAASSVSVVSNSLRLRRFRPSLAR"
     gene            676..>1778
                     /locus_tag="AC16G0_12540"
     CDS             676..>1778
                     /locus_tag="AC16G0_12540"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5100476.1"
                     /translation="MIPAFRVPSGRSVAAALYADATNLALDGASSLSFDRAGRLIRAF
                     WDGRAVRRSLDNRFIQKRKTGTYPWSYARRELDPPERDAFLQAVAHELTGIQHCLSEA
                     GAILPDEERRELGARLQDILDWSPEALEGDAGRFRSVYLPIPILPLDQYGALVVQATE
                     GCSYNQCTFCRFYRDRPFRVKSLAELRAHVHGVRGFFGEGLRLRKSIFLADANALVLH
                     PARLLEVFSLLREELPLGPGVFRGVYSFIDAFSEMPKSPEHFRALAEQGLRRVTIGLE
                     TGCDALLRFLHKPATAAETLDLVERLRAAGVHVGIIVMLGIGGDRFAGRHVSETLAVI
                     DAMRLGPQDLLYLSPLAADPDSPYRRQEKEAGI"
BASE COUNT          284 a          573 c          585 g          336 t
ORIGIN      
        1 tgttcgtggc cgtggccggc gaggtggccg gcgtggtcgc ggttgccgac acgctcaagg
       61 caagctcagt ggaggccatc cgggccctcc atgttcttgg actcgaggtg gtcatgatca
      121 ccggcgacaa ccagcggacc gctgccgcca tcgcccggca ggcgggaatc gatcgagtcc
      181 tggccgaggt cctgcccgag cagaaggcga gcgaggtgaa gaagctccag gccgagggca
      241 aggtggtggc catggtcggg gatgggatca acgatgcgcc ggccctggcc caggcggacg
      301 tcggcatcgc catcggcacg gggacggatg tggccatgga ggcctcggat atcaccctga
      361 tccgcgggga tctcaccggc gtcgtgacgg ccattgagtt gagccagcgg acactccgca
      421 cgatcaagca gaacctgttc tgggccttca tctacaacgt ccttggcatc cccatcgcgg
      481 ccggcgccct gtatcccttc ttcgggatac tcctggacac catgctggcc tcggcggcca
      541 tggcggcttc gtcggtctcg gtggtgagca actcgctccg cctccgccgg ttccggccga
      601 gcctcgcgag atagcctgat tctgggttcg tcaatcgcac ctacttcgga tcagggtgga
      661 atgacctccc gggtcgtgat cccagccttc cgggtgccct ccgggcgatc cgtggcggcg
      721 gccctgtatg cggacgccac gaatctcgcc ctcgacgggg cttcctctct gtccttcgat
      781 cgggccggcc gactgatccg ggcgttctgg gatggacggg ccgtccgtcg cagcctggac
      841 aaccgattca tccagaaacg aaagacgggg acgtacccct ggagctacgc ccggagagag
      901 ctagatccac ctgagcgcga tgccttcctc caggccgtag ctcacgagct gacgggcatc
      961 cagcactgcc tgagcgaggc aggggcgatc cttccggatg aggagcgtcg agagctgggg
     1021 gcgcgcctgc aggacattct ggattggagc ccggaggctc tggagggcga tgccgggcgc
     1081 ttccgatcgg tgtatttgcc cattcccatc ctgcctctag atcaatacgg ggccctggtt
     1141 gtgcaggcca ctgaaggctg ctcgtacaac cagtgcacct tctgccggtt ctaccgcgat
     1201 cgcccgttcc gcgtgaagtc tctcgcggag ctgcgagccc atgtccacgg tgtccgcgga
     1261 ttcttcggag agggcctacg tcttcggaag agcatttttc tcgcggacgc caacgcgctg
     1321 gttctccacc cggcgcgcct cttagaagtt tttagtctcc tccgcgagga gttgccgctc
     1381 ggtcccggtg tcttccgcgg cgtgtactcc ttcatcgacg ccttcagcga gatgcccaag
     1441 tcacccgagc acttccgtgc gctggccgag cagggactca ggcgagtcac catcggactg
     1501 gagacagggt gtgatgctct gctccgcttt cttcacaaac ccgccaccgc ggcggagacc
     1561 ttggacctgg tcgagagatt gcgagcagcg ggcgtgcatg tgggcatcat cgtcatgctg
     1621 gggatcggcg gcgatcggtt tgcgggaaga cacgtctccg agacgctagc ggtgatagat
     1681 gccatgcgac ttgggcccca ggatctcctg tacctttcgc cgctcgcggc cgatcccgac
     1741 tcgccgtacc gccggcagga gaaggaggcc ggcatcaa
//
LOCUS       DBNGZC010000730         1482 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_845735, whole genome shotgun sequence.
ACCESSION   DBNGZC010000730 DBNGZC010000000
VERSION     DBNGZC010000730.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1482)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1482)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1482
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_845735"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="AC16G0_12545"
     CDS             complement(<1..577)
                     /locus_tag="AC16G0_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR5100477.1"
                     /translation="MTYRSEISLEGRTLSIAAGRVARAADGAAWVRYGDTVVLVTAVA
                     SKQPREGIDFFPLTVDYQERAYAAGKIPGGFFKREGRPHEKETLTSRLIDRPIRPLFP
                     TGYHQDVQIIATVLSADQENDPDVLAVLGASAALTISPIPFQGPIGCVRVGRIGGRLV
                     VNPTYAQLAESDMDVVLAGSEGAVVMVEGGAN"
     gene            complement(574..843)
                     /gene="rpsO"
                     /locus_tag="AC16G0_12550"
     CDS             complement(574..843)
                     /gene="rpsO"
                     /locus_tag="AC16G0_12550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HIR5100478.1"
                     /translation="MPKDFSNKGELIGQYRLHEQDTGSPEVQIALLSGRIGYLTEHLT
                     VHKKDHHSRRGLLMMVGRRRKLLDYLKAKDVERYKRVIERLGIRK"
     gene            complement(910..>1482)
                     /locus_tag="AC16G0_12555"
     CDS             complement(910..>1482)
                     /locus_tag="AC16G0_12555"
                     /inference="COORDINATES: protein motif:HMM:NF013821.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HIR5100479.1"
                     /translation="GRRGNVELLQALAPTFGFWLEVVPPIQVDGVVVSSRLIRGLLAE
                     GRVEEAQRFLGRPYCLQGEVQPGSGRGRELGYATANVAVPAGLPLREGVYAGRVFVRG
                     VFQDAMMNLGSAPTFGPGERRLEIHMPAWEATLYGEEVSVFFLRRLRDEQRFPDAAAL
                     VRQLARDREAAAAVWEGARPLPWPDWTLHS"
BASE COUNT          243 a          552 c          455 g          232 t
ORIGIN      
        1 cgttggcccc cccctccacc atgaccaccg cgccctcgct gccggccagg accacgtcca
       61 tgtcgctctc ggccagctgg gcataggtgg ggttcacgac gaggcggccg ccgatgcgtc
      121 cgacccggac gcagccgatg ggcccctgga agggaatcgg ggagatggtg agggccgccg
      181 aggcccccag caccgccagg acgtccggat cgttctcttg gtccgccgac aggaccgtcg
      241 cgatgatctg gacatcctga tgataccccg tgggaaacag cggacggatc ggccggtcga
      301 tcaggcggga ggtcagcgtc tccttctcat gtggccgacc ctcgcgcttg aagaaccccc
      361 cggggatctt gccggcagcg taggcccgct cctggtagtc caccgtgagg gggaagaagt
      421 cgatcccctc ccggggctgc ttggaggcca cggccgtcac cagcaccacc gtatccccgt
      481 agcgcaccca ggcggccccg tccgcggccc gcgccacccg gcccgccgcg atgctaaggg
      541 tcctgccctc cagactgatc tcgctccggt acgtcatttg cggatgccca acctttcaat
      601 cacacgcttg tagcgctcca catccttggc tttgaggtag tccagcagct tgcgccgccg
      661 ccccaccatc atgagcagac cgcgtcgcga atggtggtcc ttcttgtgca ccgtcaggtg
      721 ctcggtcagg tagccgatcc gcccgctcag gagggcgatc tgcacctccg gtgagccggt
      781 atcctgctcg tgcagacggt actgaccgat gagttcaccc ttgttcgaga agtccttggg
      841 catgatcgct ctcccccctt tcgggcccaa ccgtgtcatc gacagcatca gggaacctag
      901 cataggcccc taggagtgta aagtccaatc tggccatgga aggggccgcg cgccctccca
      961 cacggctgcc gcagcctcgc gatcccgggc gagttgccga accaacgccg cggcatcggg
     1021 gaaacgctgt tcgtcccgca agcgccgcag aaagaagacg ctcacctcct cgccgtacag
     1081 ggtcgcctcc caggcgggca tgtggatctc gagccgccgc tcgcccggac cgaaggtcgg
     1141 cgccgacccc aggttcatca tggcatcctg gaagacaccc cggacgaaga ctcggccggc
     1201 gtacaccccc tcccggagcg ggagccccgc gggcacggcc acgttggccg tggcataccc
     1261 cagctcgcgt ccacggccgg atcccggctg gacctcgcct tggaggcagt agggccggcc
     1321 caagaacctc tgggcctctt ccacccgacc ctcggccaga aggccccgga tcagccggct
     1381 gctcaccacc accccgtcca cctggatcgg cggcacgact tccagccaga acccgaaggt
     1441 gggggccagc gcttggagca gctcgacgtt tccccgccgt cc
//
LOCUS       DBNGZC010000731         4984 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_258594, whole genome shotgun sequence.
ACCESSION   DBNGZC010000731 DBNGZC010000000
VERSION     DBNGZC010000731.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4984)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_258594"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="AC16G0_12560"
     CDS             complement(<1..1257)
                     /locus_tag="AC16G0_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HIR5100480.1"
                     /translation="MATQTATKKQWTMLVYLAGDNDLDGAGVVDLKEMKRVGCTQALN
                     VIAQFDRAGSKAETTRYCLQKTTALAKDAVQKLGETNMGDPKVLEDFVTWGVTKYPAE
                     HYLLVLWNHGAGWDDANLYQGDVFSGATPPVSRKNQPVVSRGAIARDGRTLAFTPMRA
                     GLARTRRALFHTTVRAAVKDRAIAFDDQAQDFLDNIELKRVMGKVKNALRRKIDILGM
                     DACLMSMVEVAYQIRDGADYTVGSEETEPGDGWPYDRILKVLAARPGMTPEELSKVIV
                     TEYLASYRAGDNVTQSAVRLASLTPLASAVDGLSRALKNALATEALRTALLTVRAQVQ
                     EYSRPYDDYCDLLDLCARLEKSMPDEMVRTACAGVKQVAQGAIVAAGYKGSAVEHSQG
                     ISIYFPKRKLSPLYTTLDFTKTSAWDE"
     gene            complement(1632..2801)
                     /gene="chrA"
                     /locus_tag="AC16G0_12565"
     CDS             complement(1632..2801)
                     /gene="chrA"
                     /locus_tag="AC16G0_12565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HIR5100481.1"
                     /translation="MPARVSYMDLCRACLWLGLTAYGGPAMVGYVRDVMVERRRWVDA
                     KEFAEGLALAQLVPGATLTQVVAYVGLLLRGPAGAAAAEACFIFPGALLVLIISAFYF
                     RWQTLPAVQVLSHGVGAVVVAILAHACLRLAKTSLPNLSTFMMAAAACLAYLLQVNGF
                     VVVMGAAGAAYIARRGWVEPVEALPEAASGVPSVSPQTWALLTGMSLAVLLGAWVLAP
                     RLARLCVAFLGIGSVAFGGGYSMIPLVQQRVVDTLGLLSTRAFMDGIALGQITPGPIM
                     LTATFIGYAAAGPAGALLATAAIFSPSWAILVVAAPRFQAWTRRRAAQAMLGGVVASF
                     VGMLLSILVRFGNAALTDVPSVLLSAGAFVALFLGVEVPLVVAVGLLLSLAAWVM"
     gene            complement(3043..4173)
                     /locus_tag="AC16G0_12570"
     CDS             complement(3043..4173)
                     /locus_tag="AC16G0_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006919788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate/molybdate transporter"
                     /protein_id="HIR5100482.1"
                     /translation="MKSTGNRYDLQELAGAFGDLGTLIPFVVGYITLNKMDPAGILVA
                     FGVFKLWAGLYFKTPVPIQPMKAIGTAAIAHPGAITPGAIWASGLFTGAVWLLLGLCG
                     AATWVAKITSRPVVQGIVLGLGLGFMLEGVKMMRGDLLLAAVMIPLTFLLLSRQRIPA
                     MLVLLAVGSVVAVVRQPGLVEELAQMSFRFRLPASGLAQIAWGDFITGVLVLGVPQLP
                     LTLGNAVISTVEENNSLFPDRPITVKTVALDHGVLNLVGTALGGVPMCHGAGGMAGHV
                     RFGARTGGALVILGGLVLFTGLFLADSVATLFKVFPPVILGVILLFGGLELASGVRGE
                     GNGKEQRYIMYLTAGLSMWNMGVGYASGLLLWYAFERKWLRA"
     gene            4611..>4984
                     /locus_tag="AC16G0_12575"
     CDS             4611..>4984
                     /locus_tag="AC16G0_12575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100483.1"
                     /translation="MSQSASLQSAEPILPGQDAFGAIAEIVRILEADPTTDWSKVNLE
                     RLRQHLIDMNEVTLRSEVKSDAVAGGLAMNVTGTGRTEQAIHRMLMAHTVELNKLPEW
                     SAKTEPIPGGMRLTVTAKNPDDA"
BASE COUNT          942 a         1649 c         1598 g          795 t
ORIGIN      
        1 ctcgtcccag gcgctggtct tggtgaaatc cagggtcgtg tagaggggcg acagcttccg
       61 ctttggaaaa tagatggaga tgccctggga atgttccacg gcggacccct tgtagccggc
      121 ggcgacgatg gccccttggg cgacctgctt cacgccggcg caggcggtgc ggaccatctc
      181 gtccggcatg gatttctcca gacgggcgca caggtcgagc aagtcacagt aatcgtcata
      241 gggccggctg tactcctgca cctgggcgcg cacggtcaat agggcggtgc gcagggcctc
      301 ggtggcgagg gcgttcttca aagcccggct caggccatcc accgcgctgg ccaagggcgt
      361 gaggctcgca agccgcacgg cggactgggt gacgttgtcg ccggcccgat aggaggcgag
      421 atactcggtc acgatgacct tggacaactc ctccggagtc atccccggcc tggcggccag
      481 gaccttcagg atccgatcgt acggccagcc gtcgcccggc tcggtctctt cggagccgac
      541 ggtgtagtcg gccccatccc ggatctgata ggcgacctcc accatgctca tgaggcacgc
      601 gtccatgccc aggatatcta tcttgcgcct gagggcattc ttgacctttc ccatcacccg
      661 ctttagttcg atgttatcca ggaagtcctg ggcctgatcg tcgaacgcga ttgctcgatc
      721 cttgaccgcc gccctcaccg tggtgtggaa cagggcgcgg cgggtgcggg cgaggcccgc
      781 ccgcatcggg gtgaaggcga gggtcctgcc atccctggca atcgcaccgc gggagacgac
      841 gggctggttc ttccgcgaga cgggcggcgt ggcaccgctg aagacgtcgc cctgatacag
      901 attggcgtcg tcccagccag cgccgtggtt ccacaggacc agcaagtaat gctctgccgg
      961 atatttcgtc acaccccaag tcacgaagtc ttccagcacc ttggggtcgc ccatgttggt
     1021 ttcgccgagc ttctgcacgg cgtccttggc gagggccgtc gttttctgca ggcagtaccg
     1081 ggtggtctcc gcctttgagc cggcccgatc gaactgggcg atgacgttga gtgcttgggt
     1141 gcatcccacc cgcttcatct ccttcaggtc cacgacgccg gccccgtcca ggtcgttgtc
     1201 ccccgcgaga tacaccagca tggtccactg cttcttcgtt gcggtctgtg tcgccatggt
     1261 gtcctcccct gcctcccggc agcctccgcg gtttgcatcc agccgagaca agacccgact
     1321 ctcaacacta ctcttgcgga ccccagcgag taccgaggtc cttggcgcct gcaacccagc
     1381 tttcgacgag gatggctgcc acaagaggcg tcagggttag gagccagagg tggggatgga
     1441 agtggtgaca acttgagtcc gccgatcccg agccggcgcc accctccaag ggataatggt
     1501 agattgcggc tatcctatat caaacggcga ggcgccgcgc aagactcgaa ccgccaggga
     1561 cgctgcgcct gcccagcgcc attcaggtcg atgaatcccg cttctccgtg acgtgatcca
     1621 ggttcgcaga atcacatcac ccacgcggcc agcgacagga gcagtccgac ggcgaccacg
     1681 agcggtacct ccacaccgag gaagagggcg acgaaggcgc cggcggagag gaggacgctg
     1741 gggacgtcgg tgagggccgc gttgccgaac cgaacgagga tggacaagag catcccgacg
     1801 aacgaggcga cgactccccc gagcatggcc tgggccgccc gccggcgagt ccacgcctgg
     1861 aagcggggcg ccgccacgac caggatcgcc cacgacggag agaagatggc cgctgttgcc
     1921 agcagggcac ccgccggtcc tgccgcggca tacccgatga acgtcgccgt cagcatgatc
     1981 ggtccgggcg tgatctgacc cagggcgatg ccgtccatga aggctcgggt ggagagcaga
     2041 cccagggtat ccacgacccg ctgctggacc agcgggatca tgctgtagcc gccgccgaag
     2101 gccacgctgc cgatcccgag gaaggccaca cacaggcggg ccagccgggg ggcgagaacc
     2161 cacgctccca ggagtacggc caggctcatg ccggtcagga gcgcccatgt ctgcgggctc
     2221 acggacggca cgccgctcgc ggcttcaggc aaggcctcca ccggttctac ccatccccgg
     2281 cgcgcaatgt atgccgcccc cgctgccccc atcaccacga cgaacccgtt cacctgcagc
     2341 aggtacgcaa gacaggcggc cgcagccatc atgaaggtcg agaggttggg caggctcgtc
     2401 ttggccagac gcaggcaggc atgggccagg atcgccacca cgaccgcccc gaccccgtgc
     2461 gagaggacct ggaccgcggg gagcgtctgc caccgaaaat agaaggcgga gatgatcagg
     2521 accagcaacg ctcccggaaa aatgaagcaa gcttcggctg cggcggcacc ggcaggaccc
     2581 cggaggagca ggcccacata ggcgaccacc tgggtaagcg tggcgccggg gacaagctgg
     2641 gccagcgcca gtccctccgc gaactctttg gcgtcgaccc agcgccgacg ctccaccatc
     2701 acgtctcgga cgtagcccac catcgccggc ccgccgtacg ccgtgaggcc gagccagaga
     2761 cacgccctac agagatccat gtaagaaact cgggcaggca tgttgctcca ccttggagga
     2821 ccaggagtcg catgagcgtc gtcagagtag gacggacgcc ctgctgtggc aatcgtatct
     2881 tcctctccgt ccgaggttcc cttcgtcggc ccagatcttt tctgctggca tgcggcaggt
     2941 cgcgcaggaa gcttcaggca tgggatggtt tggacatcgc gtgggggagg cctccgggat
     3001 cggccgatga ggttcacgtc cattcgcagg gggacgcagc ccttacgccc tcagccactt
     3061 cctctcgaac gcgtaccaca gaagcagccc actcgcgtat cccaccccca tgttccacat
     3121 ggagagcccg gcggtgaggt acatgatgta tcgctgctcc ttaccgttcc cctccccgcg
     3181 cacgccactc gccagctcca accctccgaa caggaggatc acacccagga tgaccggcgg
     3241 gaagactttg aagagtgtgg ccacggaatc ggccagaaaa agcccggtga acaggaccag
     3301 gccgccgagg atcacgaggg cgccgcccgt gcgcgcgccg aagcggacgt gaccggccat
     3361 cccgcccgcg ccatggcaca tcgggacacc ccccagtgcc gtccccacca ggttcagcac
     3421 accgtggtcc agcgccacgg tcttgaccgt gatgggccga tccgggaaga gcgagttgtt
     3481 ctcctcgacc gtgctaatga cggcattccc gagcgtgagg ggaagctgcg ggacccccag
     3541 gaccaggaca ccggtgataa aatcgcccca cgcgatctgc gcgagcccgg aggccggcag
     3601 gcggaagcgg aagctcatct gcgccagctc ctccaccagt cccggctgtc ggacgaccgc
     3661 aacgacagat cccacggcga ggaggacgag catggccggg atgcgttggc gggacaggag
     3721 caggaatgtc aggggaatca tgacggccgc gaggaggagg tccccccgca tcatcttgac
     3781 gccttccagc atgaaaccca ggcccaaccc cagcacgatg ccctggacca cggggcggct
     3841 cgtgatcttc gcgacccagg ttgccgcccc gcacaatccg aggaggagcc acacggcccc
     3901 ggtgaacagc cccgacgccc agattgcgcc cggcgtgatc gcccccgggt gggcgatcgc
     3961 cgccgttccg atggccttca tcggctggat gggcacgggg gtcttgaagt acaggcccgc
     4021 ccagagcttg aacacgccga aggctaccag gatgcccgcg gggtccatct tgttgagggt
     4081 gatgtagccg acgacgaagg ggatcaacgt gcccaggtcc ccgaaggcgc cggccaactc
     4141 ctgcagatcg tatctgttcc cggtagattt catccaacga tcctcctggc ccttgatgta
     4201 tatatatgct acagacagca tggtcaagag cggtcaccgc tcccggcagc cctcccatac
     4261 aaatcctccg tcctcgggat gcggccctag cccaaaatcc tccggggagg gctgagcatc
     4321 ggacgctcca tcttgcgacg gcgtctgaca ggcaggctgt tgcaggcata cttccgcgat
     4381 ctttcgaggt gccaggggtg ggctgggctc gttttctgct gacacgggat aattaccccg
     4441 gtacatttgg gattcgagga aacctgtgtc cagggccaat cgtgggaacc ggagtcaggt
     4501 agaggtgaac catgcataga gttgagagat tgtgtgtccc cctcgcagtc ctcacagcga
     4561 tcctcgcggc gggccccgtc ggggctcagc aaatgcagca cgaccactcc atgagtcaat
     4621 ccgcaagcct ccagtcggcc gagccgatcc tgccagggca ggacgcgttt ggcgccatcg
     4681 ccgagatcgt acgaattctc gaggccgacc ccaccaccga ctggagcaag gtgaatctcg
     4741 agcgtctgcg ccagcacctg atcgacatga acgaggtgac gttgcggtcc gaggtcaaat
     4801 cggatgccgt ggccggcggc ctcgccatga atgtgaccgg cacgggtcgg accgagcagg
     4861 cgattcatcg gatgctgatg gcccacaccg tcgagttgaa caagctgccg gagtggtcgg
     4921 cgaagacgga gccgatcccg gggggtatgc gcctgacggt gacggcgaag aatccggatg
     4981 atgc
//
LOCUS       DBNGZC010000732         4014 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2704423, whole genome shotgun sequence.
ACCESSION   DBNGZC010000732 DBNGZC010000000
VERSION     DBNGZC010000732.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2704423"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..197)
                     /gene="kdpF"
                     /locus_tag="AC16G0_12580"
     CDS             complement(105..197)
                     /gene="kdpF"
                     /locus_tag="AC16G0_12580"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HIR5100484.1"
                     /translation="MSAIYVLGGTVAVGLLIYLVIALLKPEVFS"
     gene            complement(500..796)
                     /locus_tag="AC16G0_12585"
     CDS             complement(500..796)
                     /locus_tag="AC16G0_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100485.1"
                     /translation="MRRPKPAWGLLYAVFLLAVVLLVAAHLEAPSPSWREAADGVASL
                     LVIGAMALWVRANRLALLRSGESTTDGEGLRVWVAHSPQPLPRREGSLAEIEGN"
     gene            982..1284
                     /locus_tag="AC16G0_12590"
     CDS             982..1284
                     /locus_tag="AC16G0_12590"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR5100486.1"
                     /translation="MGRLGRSDLIRVLAQEPNPVVEARTDAELVADMQAQFAQEDWVS
                     NRTLGIHARNGVIVLSGLVANEEERAALENLARAIADCTGVEDRLIPRTAVRRAWA"
     gene            complement(1538..2884)
                     /locus_tag="AC16G0_12595"
     CDS             complement(1538..2884)
                     /locus_tag="AC16G0_12595"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5100487.1"
                     /translation="MPPGNSLLELLHVSQVFTGGEKVFTAIQSVDLTLKEGELAVLVG
                     PSGCGKSTLLRIITGLQRPTEGLVLYRGSSVKGVNPQATIVFQTFALFPWLTVQENVE
                     VALKARGVSPELRRARALEVLDRVGLDGFETAYPRELSGGMRQKVGFARAMAVEPELL
                     CLDEPFSALDVLSAEALRGELLELWTSGKIPTRAILMVTHNIEEAVFLGDRIVVMEKG
                     PGRVVADLRVGLPQPRHRKAPEFLEMVDRVYATLAGQTQPEPVELGSAPGEGGRTRAL
                     PHVPINSLAGLLEHLAERPSHQADLYRLAEELHVNSDNLLWLSEAAELLGFATISRGD
                     ITLTPLGETFAEASILARKEIFASRIRRLPMFRWLLTMVQAAHRQRLEWEVAKAALEL
                     EFPPEEAERQLDTAVNWGRYTELLAYDDNDRVFYLEPSGVSSTETMPKADGSPGSP"
     gene            complement(2889..>4014)
                     /locus_tag="AC16G0_12600"
     CDS             complement(2889..>4014)
                     /locus_tag="AC16G0_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014434384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5100488.1"
                     /translation="GWFFLMAAETFTVGQRDFRLPGLGSYLQAAASQGDARAITWGVL
                     ALVVVVIALDQLVWRPLLTWADRFKLEMVEGETPPASWVTEVLRSSHLMDRLHQLWEP
                     VAERLDAWLFRRFPGAASTSGTGAGPSMGLYVAGIAGGLALLYGGSHAGQMLVAVPLA
                     EWGTMALGLLATLLRVLVALLIALAWTVPLGVAIGTSPRLAAWLQPVVQIAAAVPATA
                     LFPVLILMVAGLPGGLNLAAVLLMLLGTQWYLLFNVIAGTTAIPQDLKYTAALMQLSR
                     LERWRTLTLPALFPHIVTGAITASGGAWNASIVAEYAEFGGRLLTTRGIGSIIAQATA
                     QGDYPVLLAGTLSMILVVVGIHRVVWRPLSRLAETRYHLE"
BASE COUNT          785 a         1314 c         1282 g          633 t
ORIGIN      
        1 ggtggggcgg ccctcgtaca cgcgggccat gtaggcgccg agtggcttga caagcgccag
       61 caagacgcca aagaacacca caaattggag aacgccattt gcggtcatga gaagacctcc
      121 ggcttgagca gcgcgatcac aaggtagatc agcagcccaa ctgcgacagt cccgccgagg
      181 acgtagattg ctgacattgt tccctcctac actcgctcac agagaacgac gaacaaccac
      241 gtgagcccga aaaagacgac ggtaatcccg acgaaaagca gatccatggg catgtcctcc
      301 tctcaccggc cgggaatcta ccctgaccct cgaagggttg ggttacctca ggtgctccag
      361 cgcgagcccc acccgcagga gcaggcctgc gctggcgatc agggtcatca cgatcagaga
      421 ggcaatggct gacttgatct ggctcgacat ggcgcatcct gcttgtccac gtcccctggt
      481 ccggggcgtg tttcctgatt caattgcctt cgatctcggc gagactgcct tcccgccgcg
      541 gcaatggctg cggggagtgg gcaacccaca cgcgcaggcc ctccccgtcc gtcgtggatt
      601 ctccagaccg cagcaaggcc agccggttcg cccgcaccca gagggccatt gccccgatga
      661 caaggagcga ggccactcca tcggcggcct ccctccagct aggcgacggc gcctcgagat
      721 gggctgccac cagcagcacc accgcgagca gaaacactgc gtagagcagt ccccatgcag
      781 gcttcggtct tcgcatcatc aatctcctgc cacctgacat ggcttcgtgg tctcctggct
      841 ttcggcttca gaaggaatct agaagggtag cgcatgagtc tcaagtcaag aggtcgtcaa
      901 aaacagaggc gggagtatca agaagtgtca agattcgcga atgaggattc gtgaggtgcc
      961 tgttatgctc ggggggcact cgtggggagg ctgggccgga gcgacctcat ccgggtcctg
     1021 gcgcaagaac ccaaccccgt ggtcgaagcg cggaccgatg ccgagctcgt cgccgacatg
     1081 caggcccagt tcgcccagga ggattgggtc tccaaccgga cgctcgggat tcatgcgagg
     1141 aatggggtga ttgtcctctc cgggctcgta gccaacgagg aggagagagc cgccctcgaa
     1201 aacctggcgc gcgccatcgc agactgcaca ggcgtcgagg accgcctgat accacggacg
     1261 gcggtccgcc gagcctgggc ctagcctcct ctacaagcaa cgcgacaagc cgagtcgcga
     1321 cggcctgaca ttgtcctcgc ggacctcggc ctcctgacct cgtcggactg gacgtgaatc
     1381 agcgcttgcg tgaatggacc gcagtgccga tcatcctgtg cctagtgcgc tgcaaagtga
     1441 cgacgggcct cgaaactttg atgctgctct gcaagcaaag atggacgggc caagcacgga
     1501 acatgctcag gtggagcacg cctctgccag tcatccttca tgggctgccg ggcgatccgt
     1561 ctgctttggg cattgtctcg gtggatgaga caccgctggg ttccaaatag aacactcgat
     1621 cgttgtcgtc gtacgccagc aactcggtat accgacccca gttgacggcg gtgtccaatt
     1681 ggcgctcggc ttcttccggc gggaattcca gctccagggc cgccttggcc acctcccact
     1741 ccagccgctg ccggtgggcc gcctgcacca ttgtgaggag ccaccggaac atcggtaacc
     1801 gccggatacg gctagcgaaa atttccttcc gggccaggat gctggcctcg gcaaaggtct
     1861 cccccaatgg ggtcagcgtg atgtcacctc tggagatcgt ggcgaacccc aagagctccg
     1921 ccgcctcgga cagccacaaa aggttatctg agtttacgtg aagctcctct gccagccggt
     1981 agaggtcggc ctggtgggag gggcgttcgg ccagatgctc cagcaacccg gccaggctgt
     2041 tgatggggac atgcggcaaa gcgcgcgtcc gacccccctc gccgggggca ctgcccagct
     2101 ctacaggctc cggctgggtc tggccggcca gggtggcgta cactcggtcc accatctcca
     2161 gaaactccgg tgccttgcgg tggcggggct gaggaagtcc aacccgcaga tcagccacca
     2221 cgcggccggg gcccttttcc atcacgacga tgcgatcccc gaggaagacc gcttcctcga
     2281 tgttgtgggt gaccatgagg atggcgcgcg tcgggatctt gccgctcgtc cacaattcca
     2341 gcaactcccc gcgcagcgcc tccgcgctca ggacatcgag ggccgagaag ggttcgtcca
     2401 ggcagagcag ttcgggctcc accgccatgg cgcgggcgaa cccgaccttc tggcgcatcc
     2461 cgcccgacag ctcccgcgga taggctgtct cgaatccgtc taggcccacc cggtccagga
     2521 cctccaatgc ccgagccctg cggagttcgg gagacactcc ccgcgccttc agggcaacct
     2581 ccacgttctc ctggaccgtc agccaaggga acaaggcaaa cgtctggaag acgatggtgg
     2641 cctgggggtt cacccccttc acggatgagc cacggtagag caccagaccc tcggtcgggc
     2701 gctggaggcc cgtgatgatg cgcaagagag tactcttccc gcagcccgac gggccgacga
     2761 gaaccgccaa ctccccctct ttgagggtca ggtctacaga ctggatagcg gtgaagacct
     2821 tttcgccgcc ggtgaagacc tggctgacgt gaagcaactc tagaagagaa ttcccgggcg
     2881 gcatgggatc actccagatg gtaccgggtt tcggccagac ggctcagtgg ccgccagacc
     2941 acacgatgga tgccgacgac caccaggatc atgctgagcg taccggccag gagcacaggg
     3001 tagtcccctt gggctgtggc ctgggcgatg atcgaaccga tcccacgagt ggtgaggagt
     3061 cggccgccga actcggcgta ctccgcgacg atgctggcgt tccaggcccc gccgctggcc
     3121 gtgatggccc ccgtcacgat gtgggggaac agggcgggta gggtcagcgt gcgccagcgc
     3181 tccaggcggc tgagctgcat aagggccgcg gtgtacttca aatcctgggg gatggcggtg
     3241 gtgcccgcga tcacattgaa taggaggtac cattgggtgc ccaggagcat cagcaacacg
     3301 gccgccagat tgaggccgcc cggcaagcct gcgaccatca ggatgagcac agggaatagt
     3361 gccgtggccg ggaccgcagc ggcgatctgg acaacgggtt gcaaccaggc tgccaggcgc
     3421 gggctggtcc cgatggccac ccccagggga acggtccagg ccagggcgat gagcagggcg
     3481 accaggacgc gcaggagcgt ggcgaggagg ccgagcgcca tggttcccca ttccgccagg
     3541 gggacggcca cgagcatctg gccggcgtgg gagcctccgt agagcaacgc cagccccccc
     3601 gcgattcccg cgacatacag acccatcgac gggccggctc ccgtgcccga ggtgctggcc
     3661 gcaccgggaa agcggcgaaa caaccaggca tcgagccgct cggccaccgg ctcccagagc
     3721 tggtggagcc ggtccatcag gtgtgaactg cgcaagacct cggtgaccca cgaggccggc
     3781 ggggtctcgc cctcgaccat ctcgagcttg aaccggtccg cccacgtcaa cagcggccgc
     3841 cacacgagtt gatccaaggc gatcacgacg acgaccaaag cgaggacacc ccaggtgatg
     3901 gcccgggcgt ccccctgact ggcggccgct tgcagatacg acccgagccc gggcagccgg
     3961 aagtcgcgct gccccacggt gaaggtctcg gccgccatca agaagaacca gcca
//
LOCUS       DBNGZC010000733         3030 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2506394, whole genome shotgun sequence.
ACCESSION   DBNGZC010000733 DBNGZC010000000
VERSION     DBNGZC010000733.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2506394"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            490..1371
                     /locus_tag="AC16G0_12605"
     CDS             490..1371
                     /locus_tag="AC16G0_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR5100489.1"
                     /translation="MRRFRIAVAQIAPTLGDLEKNLALHEKMAREAISHSANLLVFPE
                     LSLTGYFLKDQVPSVSLCEGAPALRPLLDLSQRVAMVVGFVEEGPGHLFHNAAAYLEG
                     GEIRHLHRKVYLPTYGIFDEQRYLAAGGRIRPFDTALGRMTILICEDLWHPAAVGIAA
                     WDGAEVILCPSASPGRGQGEAAPFQNSATWERVIRAYGDLFTCFVVYANRVGYEDGAC
                     FWGGSEVVEPTGHTLSKARYLDEELLLADVEPGLLRRARITNPLLRDERLDLTLRELN
                     RIVREREEQTGMGGNGL"
     gene            1477..2301
                     /locus_tag="AC16G0_12610"
     CDS             1477..2301
                     /locus_tag="AC16G0_12610"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HIR5100490.1"
                     /translation="MVNLQLKCETVRKVLVGFIDNEVRKAGFERVVVGLSGGVDSTLS
                     AFLAAEALGPQNVWGILMPYRTSNPESAEHATLVIRALHINSLTVDITPMVDAYFAMF
                     PDADQVRRGNKMARERMTILFDHSARLRALVLGTSNKTELLLGYGTLHGDMASAVNPL
                     GDLYKTQIRQLARHMQVPTVIVEKQPSADLWVGQTDEAELGFTYEEVDRVLHLLVDQR
                     YEVADLVREESFDEKFVRSVSRKIQASQYKRRLPVIAKVSSRTIDRDFRYPRDWGR"
     gene            2406..>3030
                     /locus_tag="AC16G0_12615"
     CDS             2406..>3030
                     /locus_tag="AC16G0_12615"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - L-alanine dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIR5100491.1"
                     /translation="MIIGIPKEIKDNENRVGAIPAGVQALVSRGHTVLVQKGAGLGSG
                     LSDQTYETAGAKIIRPARQVYKDAEMILKVKEPLPSERGLLQPDQVLFTYLHLAPDLE
                     LTKALLRRKIVGIAYETVETADGRLPLLEPMSEVAGRMAVHTAAYYLAKPFGGRGVLM
                     SGVPGVPPATVVVLGGGIVGYNAARIAAGMGARVYLLDVNALRMRYLD"
BASE COUNT          537 a          923 c         1029 g          541 t
ORIGIN      
        1 ctggagaaga gaatggttgc ccaaagaaaa ggccgggcct ccatcttcct ggaggacacc
       61 ggccgcatgg aacccagcac tctccggaac cggggcagga tctcgcctcc ggttgggtgg
      121 tcaagaaatc gcttcacacg accatctttg gaactggaaa agacatctac ctcaaagcgg
      181 tccgggtacc cggcgtctgc gctgggtgac ctttgcggct caagatcttc ttggattgtg
      241 gctgactcgt caagcgcgtc atccggtagg gggcatcgac agtccgggcg caggggatcc
      301 ggcgtatcgc attcagcttg ccggctgcct gaccaggata tattgcatgt ccgtgacgac
      361 ccccgtggac aatacaggag gcactcctct agcttgttca gtggtggggc agttgagttg
      421 ggacggggag tgtagtagga tgctgcgagt tcgcgtgttg agagggcgcg aagccttctg
      481 gggtaatcta tgcgacgatt tcgcattgcc gtggctcaga tcgcgccgac gctgggcgat
      541 ctggagaaga acctcgccct ccacgagaag atggcgcggg aggccatatc ccattcggcc
      601 aacctgctcg tctttcccga gctcagcctg accggctatt tcctcaagga tcaggttccc
      661 tcggtctccc tctgcgaggg ggcgcccgca cttcgcccct tgctcgatct gagccagcgg
      721 gtggccatgg tggtgggctt cgtcgaggaa ggtccgggcc acctttttca caacgcggcc
      781 gcctatctgg agggcggcga gattcgccac ctccatcgca aggtctacct gcccacatac
      841 gggatcttcg atgagcagcg atacctggct gccggcggtc ggatccgccc cttcgatact
      901 gcgctcggcc ggatgaccat cctcatctgt gaagacctct ggcatcccgc tgccgtcggg
      961 atcgcggcct gggacggggc cgaggtgatc ctctgcccct cggccagccc aggtcgcggc
     1021 cagggagagg cggcgccctt ccagaactcc gccacctggg agcgcgtcat ccgcgcctac
     1081 ggcgacctgt tcacctgctt cgtggtgtac gccaaccggg tcgggtacga ggatggtgcc
     1141 tgcttctggg gcgggtcgga ggtcgtggag cccaccgggc acaccctgag caaggcacgc
     1201 tacctggacg aggaattgct cctggcggac gtcgagccgg gacttctgcg gcgggcgcgt
     1261 atcaccaatc ccctcctccg ggacgagcgg ctcgacttga cgctgcgtga gctcaaccgg
     1321 atcgtgcggg agcgcgagga gcagacgggg atgggaggta atgggttgta acgggtggtc
     1381 acggatggtg acggattgta acggatggtc acggatggca acggatggca acggatgggg
     1441 agggcgttgg tgggcggtgg ggggtcggcg taacccatgg tgaatctcca gctcaagtgt
     1501 gagacggtcc gcaaggtcct tgtcggcttc atcgacaacg aggtgcggaa ggccggattc
     1561 gaacgggtgg tggtcggcct ctcgggcggc gtggactcga ccctgtcggc gttcctggca
     1621 gcggaggcgc tgggaccgca gaacgtctgg ggcatcctga tgccctatcg cacctccaac
     1681 cccgagagcg cggagcacgc cacactggtg atccgggcgc ttcacatcaa cagcctgacc
     1741 gtggacatca cgcccatggt ggacgcctac ttcgccatgt tcccggatgc ggaccaggtc
     1801 cggcgcggga acaagatggc tcgcgagcgc atgaccatcc tcttcgacca ctcggcccgc
     1861 ctgcgggcat tggtcctggg gaccagcaac aagacggagc tgctgctggg gtatggaacg
     1921 ctccacggcg acatggcctc ggccgtgaat cctctgggcg acctctacaa gacacagatc
     1981 cggcagctcg cccggcacat gcaggtgccg acggtcatcg tggagaaaca gcccagcgcg
     2041 gacctgtggg tgggccagac ggacgaggcc gagctggggt tcacctacga ggaggtggat
     2101 cgggtcctgc acctcctggt ggatcagcgc tatgaggtgg ccgacctggt ccgcgaggaa
     2161 tccttcgacg agaaattcgt ccggtcggtc tccaggaaga tccaggcttc tcagtacaag
     2221 cggcggctgc ccgtcatcgc caaggtatca tcccgcacca tcgatcgcga cttccggtac
     2281 ccgcgtgact ggggacgcta gaccgggcga tttggcgatc agacgactcg acagttcggc
     2341 catccgcact cgggatccgg gtccgtgcct cgttgccaaa taacctcatt gccagggggc
     2401 ggccaatgat catcggcatt ccgaaagaga tcaaggacaa cgagaatcgg gtgggggcga
     2461 ttcccgcggg agtccaagcg ctggtgtcgc gcggccacac ggtcttggtc cagaaggggg
     2521 ccggcctcgg atcgggcctc agcgaccaga cctatgagac agccggcgcc aagatcatcc
     2581 gaccggcgcg ccaggtctat aaggacgcag agatgatcct gaaggtgaag gagccgcttc
     2641 catcggagcg aggacttctc caacccgacc aggtcctctt cacctacctg cacctggcgc
     2701 ccgatctgga actcacgaag gctctcctgc gccggaagat tgtcgggatc gcctacgaga
     2761 cggtggagac ggcggacggc cgactgcccc tcctggagcc gatgagcgag gtggccggac
     2821 gcatggcggt gcacaccgcc gcctactatc tcgccaagcc cttcggcggc cggggcgtcc
     2881 tgatgagcgg tgtgccgggg gttccgccgg ccacggtcgt ggtcctaggc gggggcatcg
     2941 tggggtacaa tgcggcccgg attgccgccg ggatgggggc gagggtctat cttctggatg
     3001 tgaacgcgct caggatgcgc tacctggacc
//
LOCUS       DBNGZC010000734         2484 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1725493, whole genome shotgun sequence.
ACCESSION   DBNGZC010000734 DBNGZC010000000
VERSION     DBNGZC010000734.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1725493"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..1389
                     /locus_tag="AC16G0_12620"
     CDS             55..1389
                     /locus_tag="AC16G0_12620"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR5100492.1"
                     /translation="MGSESYFDWTARLFDRERIFEKPEALRGIRVLDLSIIIFGPATA
                     DYLGEMGAEVIRVEMPGAGDVMRIVAPRGSYWKNVSPAFFAQNHNKYHVAIDVRRPEG
                     QALIKGLAAKSDILVENFRGGTLERKWGIGYRQLREINPRLIYVANTGFGQWGPYIKR
                     ASYDALAQAVSGFTAVSGFPGRPPLKIGIYIGDYFGACLSALGVLAALHHRDRTGQGQ
                     FLEVGQCEALLRAMDWTWVYAGLTGKNRSQTGNRDPSACPSGIYACKDGFVAVVAGSD
                     EAFKGLCRAMGREDLLRDPRFLTRADRMREPHAEALNGCIEAWVREMTRGGVEAQARQ
                     HGFSAAAVATAKDHYEDAHLQARGSVWKMEDPVYGTMVEHGPAPKLSETPARYKWAAK
                     PVGFHNEHVLGRLLGLRPDQLAQLTRQGVIGKWADRRGPRPPEGLDGEGIIE"
     gene            1497..>2484
                     /locus_tag="AC16G0_12625"
     CDS             1497..>2484
                     /locus_tag="AC16G0_12625"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIR5100493.1"
                     /translation="MSELTWEDWIRQADDPRDAFAKPEALDDLVVLDVSRESVAGMVC
                     SAFLAELGAEVLKTEPPGGDPARLWGPPDFMHRGEGLAYLAEGRNKYYITLSLEEESG
                     REIFRHLARRADVVIEAELPGRMDAWGIGYRQLSAENSRLVYVAISAQGQFGPRAHEG
                     VQEHELTSQATSGLTFITGEPPTGDACPAAVPTKAGPWLAGYAGGAWAAFAILAALHH
                     RHLTGQGQMVDVTPAEGLMRYLEYTLLWYHVDRRIRERIGYYDLAVFPYTFVRVKDGW
                     AFIAAYTDPNFQALCRIMGRPELAEDERFSGVWQRVQAENEEALRREIEAWSV"
BASE COUNT          427 a          797 c          864 g          396 t
ORIGIN      
        1 gctgaccgcc gaaggcggaa cctcacggaa gattgcagag aggcgaggcg tgccatgggg
       61 tcggagtcct acttcgactg gactgctcgg ctgttcgacc gggagcgcat cttcgagaag
      121 ccggaggcgc tacgcggcat ccgcgtcctg gacctgtcca tcatcatttt cggcccggcc
      181 accgccgact accttggcga gatgggggcg gaggtgatcc gggtcgagat gcccggcgcc
      241 ggcgacgtga tgcggatcgt cgccccgcga ggctcctact ggaagaacgt ctcccctgcc
      301 ttcttcgccc agaatcacaa caagtaccac gtggccatcg acgtccgacg ccccgagggc
      361 caggcgctca tcaaggggct ggcggccaag agcgacatcc tggtcgagaa cttccgcggc
      421 gggacgctgg agcggaagtg gggcatcggc tatcgccagc tccgcgaaat caatccccgt
      481 ctgatctacg tggccaacac aggcttcggc cagtggggac catacatcaa acgcgcctcc
      541 tacgatgccc tggcccaggc ggtgagcgga ttcacggctg tctcggggtt tccggggcgg
      601 ccgccgctca agatcggcat ctacatcggc gactacttcg gcgcctgcct ctcggccctg
      661 ggtgtcctgg ccgcgcttca ccaccgggat cgaaccggac aggggcagtt cctggaagtc
      721 ggacagtgcg aggccctgct gcgcgccatg gactggacct gggtctatgc ggggctcacc
      781 ggcaagaacc ggtcccagac cgggaaccgc gacccatcgg cctgtccctc cggaatctat
      841 gcctgcaagg acgggttcgt ggccgtcgtc gccggaagcg acgaggcttt caagggcttg
      901 tgccgggcca tggggcgtga ggacctcctg cgcgaccccc gcttcctgac gcgggcggat
      961 cgcatgaggg aaccccatgc cgaggcgctg aacgggtgca tcgaggcctg ggtgcgtgag
     1021 atgacgcggg gtggggtgga ggcacaggcc aggcagcacg ggttctccgc cgcggccgtc
     1081 gccacggcga aggaccacta tgaggatgcc caccttcagg cccgaggctc cgtctggaag
     1141 atggaggatc ccgtgtacgg gaccatggtg gagcatggcc ccgcacccaa gctgtccgaa
     1201 acccccgcac ggtacaagtg ggcggccaag ccggtcggtt ttcacaacga gcatgtcctg
     1261 ggaaggctcc tgggtctccg tccggatcag ctggcccaat tgacacggca gggcgtcatc
     1321 ggcaagtggg ccgaccgccg cggcccaagg ccgccggagg gattggacgg cgaaggtatc
     1381 atcgagtgac gcagggagca cagggcatag ggcagcgggc gatcttcgat gggttcaccc
     1441 taatcgccga ttgctgatcg ctgacctctg acttctgacc tggtctctgg gcgagcatga
     1501 gcgagctaac ctgggaagac tggatcaggc aggcggatga ccctcgcgac gcctttgcga
     1561 agcccgaggc gctggacgat ctggtagtcc tggacgtgag ccgggagagc gtcgccggga
     1621 tggtctgctc cgcctttctg gcggaactgg gggccgaggt gctgaagacg gagccccccg
     1681 gaggcgatcc ggcgcgcctg tggggaccgc cggacttcat gcatcgcggc gaggggctgg
     1741 cctacctcgc cgaggggcga aacaagtatt acatcaccct gagcctcgaa gaagagtcgg
     1801 ggcgggagat cttccgccac ctcgcccggc gggccgatgt cgtgatcgag gcggagctac
     1861 ccggccggat ggacgcatgg ggcatcggct atcgccagct ctcggccgag aactcacggc
     1921 tggtctacgt ggccatctca gcacagggtc agtttgggcc gcgggcgcat gagggcgttc
     1981 aggaacacga gctcaccagc caggcaacct ccggactcac cttcatcacg ggcgagcccc
     2041 cgaccggcga cgcctgcccg gccgccgtcc cgaccaaggc tgggccgtgg ctggccggat
     2101 acgccggggg tgcctgggcg gccttcgcca ttctggcggc cctgcaccac cgccatctga
     2161 ccggccaggg acagatggtg gacgtgacac cggccgaagg tctcatgcgc tacctggagt
     2221 acaccctcct ctggtaccac gtggacagga ggatccggga gcggatcggc tattacgacc
     2281 tggcggtctt cccctacacc ttcgtgcgag tgaaggatgg ctgggcgttc atcgccgcct
     2341 acaccgatcc caacttccag gcgctctgcc ggatcatggg tcgcccggaa ttggccgagg
     2401 atgagcggtt ctccggggtg tggcagcgag tccaggccga gaacgaagag gccctccgtc
     2461 gggagatcga agcctggtcg gtcc
//
LOCUS       DBNGZC010000735         1689 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1238701, whole genome shotgun sequence.
ACCESSION   DBNGZC010000735 DBNGZC010000000
VERSION     DBNGZC010000735.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1238701"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..433
                     /locus_tag="AC16G0_12630"
     CDS             53..433
                     /locus_tag="AC16G0_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclophilin-like fold protein"
                     /protein_id="HIR5100494.1"
                     /translation="MATERKVRITAGKVSAEALLNDSPTATKIWEALPIEWRGSTWGD
                     EIYFSIPVEAKPEKDARDIVAVGELGYWPPGTAFCIFFGRTPASTDERPRAASAVNIL
                     GKVQGDATAFKAVSSGTRVRLERA"
     gene            430..>1689
                     /locus_tag="AC16G0_12635"
     CDS             430..>1689
                     /locus_tag="AC16G0_12635"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR5100495.1"
                     /translation="MRHESLRLPPGGEDLETLDGFLQNVLQREASRGHLVHIEAIPTR
                     GPRYAEPEPPLPGPIQQALRAAGIQRLYTHQVEALTHARAGKDVLVVTGTASGKSLAF
                     QLPVLETLLCDPEARALFLFPTKALEQDQLKSLQQLLPWGSGIRAEILDGDTPAHRRA
                     QLKEQLPHILLSNPDILHLSLLPYHAAWKGFWENLRWVVLDELHTYKGIFGSHIAHVL
                     RRLHRVTAFYGSRPQFITCSATISNPSEFAGTLAARDFIRVDDDGSPARGKRFVFVNP
                     SRSPYGEATELLLHCIRSGFKTIAFAKARKIAELITMWAHQAAPELRAKIRAYRAGYR
                     PEERRAIEQGLFHERLLGVVSTSALELGIDVGGLDACLLVGYPGSIASTWQRGGRVGR
                     AGQEALIILIALPDALDQYFMRHPHDFF"
BASE COUNT          276 a          624 c          529 g          260 t
ORIGIN      
        1 atccgcggat gcgatagatt ccgaaccggt cccttaccag gaggttttct gcatggccac
       61 agagcgcaag gtccggatca cagcagggaa ggtgagtgcc gaggcgcttc tgaacgattc
      121 gcccacggcc accaagatct gggaggccct acccatcgag tggcgcggga gcacctgggg
      181 cgacgagatc tacttctcca tcccggtgga ggccaagccg gagaaggatg cccgcgacat
      241 cgtcgccgtg ggcgagttgg gctactggcc ccccgggacc gccttctgca tcttcttcgg
      301 tcggaccccg gccagcaccg acgagcggcc gcgcgccgcc agcgcggtga acatcctcgg
      361 caaggtccag ggcgacgcca ccgctttcaa ggcggtctcg tccgggacgc gggtgcgact
      421 ggaacgggcg tgaggcacga gtccctgcgc cttcccccgg ggggcgagga tctcgaaaca
      481 ctggacggct tcctgcagaa cgtcctccag cgcgaggcga gtcgcggcca cctggtccac
      541 atcgaggcaa tccccacgcg ggggccccgg tacgcggagc cggagcctcc gctccccggt
      601 ccgatccagc aggctctccg cgccgcgggc atccagagac tctacaccca ccaggtcgaa
      661 gcgctgaccc acgcccgcgc cgggaaagac gtcctggtcg tcacggggac cgcctcggga
      721 aagagcctcg ccttccaact ccccgtcctg gagaccctgc tgtgtgaccc cgaggcccgc
      781 gccctcttcc tgttccccac caaggcgctg gagcaggacc aactgaagtc gctgcagcag
      841 ctcctcccct ggggctcggg gatccgggcg gagatcctgg atggggacac gccggcccac
      901 cgccgggcgc aactcaagga gcagttgccc cacatcctcc tcagcaaccc ggacatcctg
      961 cacctgtccc tgctcccgta ccatgccgcc tggaagggct tctgggagaa tctgcgctgg
     1021 gtcgtcctgg acgagctaca tacctacaag ggcatcttcg gctcccacat cgcccacgtc
     1081 ctgcgacgcc tccaccgcgt gacggccttt tacggcagcc gcccgcagtt catcacctgc
     1141 tcggccacca tcagcaaccc ttcggagttc gccgggactc tggccgcgcg cgacttcatt
     1201 cgggtggacg acgacggctc cccggcccgg ggcaagcgct ttgtcttcgt gaaccccagc
     1261 cggagtccct acggtgaggc caccgaactc ctgctccact gcatccggtc cggcttcaag
     1321 accatcgcct tcgccaaggc tcggaagatc gccgagctca tcaccatgtg ggcccaccag
     1381 gccgccccgg agctgcgggc caagatccgc gcctatcgcg ccggctaccg ccccgaggaa
     1441 agacgagcca tcgagcaggg cctcttccac gagcggctcc tgggcgtcgt ctccacctcg
     1501 gccctggagc tggggatcga cgtggggggg ctggatgcct gcctcctggt gggctatccg
     1561 ggaagcatcg ccagcacctg gcagcgcggc ggccgcgtgg ggcgggcggg gcaggaggcg
     1621 ctgatcatcc tcatcgctct ccctgacgcc ctggaccagt acttcatgcg acacccccac
     1681 gacttcttc
//
LOCUS       DBNGZC010000736         1712 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_454812, whole genome shotgun sequence.
ACCESSION   DBNGZC010000736 DBNGZC010000000
VERSION     DBNGZC010000736.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_454812"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="AC16G0_12640"
     CDS             <1..1360
                     /locus_tag="AC16G0_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HIR5100496.1"
                     /translation="TSPEDIGGMHAAQGILTARGGMTSHAAVVARGMGKPCVAGSGDI
                     TVYEEHGYFFAKDFKIQRGDYITLDGGSGDVMLGEAKLIQPELSGDFATLMNWADKYR
                     KIGVRTNADTPTDARVTMRFGAGGIGLTRTEHMFFEGDRIDAVREMILAEDVEGRKRA
                     LAKLLPMQKADFKAILEIMGNLPVTIRTLDPPLHEFLPKTEADIEDLARKVNIPVERL
                     KAKVDGMHEFNPMLGYRGCRLGIEYPEITEMQARAIFEAACELRKAGKNPYPEIMIPL
                     VGTLQELQLQQDIVERVAQECMKAAGVKVRYMLGTMIEIPRACLVADQIAKLAEFFSF
                     GTNDLTQTILALSRDDAGKFLPLYVERGVYAEDPFVSIDQEGVGAMMKMAIEKGRKTR
                     KDLEIGICGEHGGEPRSVIFCHEIGLDYVSCSPYRVPIAKLAAAHANLKGQVKVKEMG
                     EK"
BASE COUNT          344 a          516 c          583 g          269 t
ORIGIN      
        1 gacctcgccc gaggatatcg gcggcatgca cgcggcccag ggcatcctca cggcccgggg
       61 cggcatgacc tcccacgcgg ccgtggtggc gcgcggcatg ggcaagccct gcgtggccgg
      121 ctccggcgac atcacagtgt acgaggagca cggctacttc ttcgccaagg acttcaagat
      181 ccagcggggg gactacatca cgctggacgg cgggtccggc gacgtgatgc tgggcgaggc
      241 caagctgatc cagccagagc tgtccggcga cttcgccacg ctgatgaatt gggccgacaa
      301 gtaccggaag atcggcgtgc gcaccaacgc cgacacgcca accgacgccc gggtcaccat
      361 gcggttcggg gccggcggca tcgggctcac ccggacagag cacatgttct tcgagggcga
      421 ccgcatcgac gcggtgcgag agatgatcct ggccgaggac gtggagggga gaaagcgcgc
      481 cctggccaaa ttactgccca tgcagaaggc ggacttcaag gccatcctgg agatcatggg
      541 caacctgccg gtgacgatcc gcacgctgga tccgcccctg cacgagttcc tgcccaagac
      601 ggaggcggac atcgaggacc tggcccggaa ggtgaacatc cccgtggagc ggttgaaggc
      661 caaggtcgac ggcatgcacg agttcaaccc catgctcggc taccgcggct gccgcctggg
      721 gatcgagtac cccgagatca ccgagatgca ggcccgggcc atcttcgagg ccgcttgcga
      781 actccggaag gcggggaaga acccgtaccc cgagatcatg atcccgctgg tggggacgct
      841 gcaggagctg caactccaac aagacatcgt ggagcgcgtg gctcaagagt gcatgaaggc
      901 ggccggcgtg aaggtccgct acatgctcgg gaccatgatc gagatcccgc gggcctgcct
      961 ggtggcggat cagatcgcca agctggcgga gttcttctcc ttcggcacca atgacctcac
     1021 ccagaccatc ctggcgctct cccgtgacga cgccggcaag ttcctgccgc tgtatgtgga
     1081 gcggggggtc tacgccgaag acccgttcgt gagcatcgac caggagggtg tgggggccat
     1141 gatgaagatg gccatcgaga agggccgcaa gacccgaaag gacctggaga tcgggatctg
     1201 cggggaacac gggggtgagc cgcggtcggt catattctgc cacgaaattg gcctggacta
     1261 cgtgagctgc tccccctacc gcgtccccat cgccaagctg gccgcagccc atgccaactt
     1321 gaagggccag gtcaaggtga aggagatggg cgagaagtaa ggctgcatca cgatctggct
     1381 catccatcgg ggggccgaca tttcggcccc ccgttgtttt cgggccccct gcatggatca
     1441 ctcctgcttg caccttggac aggagcttgg tataagcttt ttgccggcag gagacagggg
     1501 cgaccatgac ggccctgggg ggtagatcat ggccattcga ggatgcccaa ggtggaggag
     1561 tcggcgaaac agctgagtgg ccgatgacgc agagaaagcc gcaaccaaag gcataatcat
     1621 gacagaaccg actgcgcttc agacattgca ggatcgattc gcgattcccg gggttgtgcg
     1681 gttcgagatg ggggaacgtg ggctgattcg cg
//
LOCUS       DBNGZC010000737         2533 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_162721, whole genome shotgun sequence.
ACCESSION   DBNGZC010000737 DBNGZC010000000
VERSION     DBNGZC010000737.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_162721"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1391
                     /locus_tag="AC16G0_12645"
     CDS             <1..1391
                     /locus_tag="AC16G0_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006920375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HIR5100497.1"
                     /translation="SIGQFNQWVGGQLMSSGTVQIWPGTPVSKPLIEDGRVVGVRLID
                     QGTDLKGNPEAGFMPGMDIRAALTVVGDGPVGAIGRQLDEQFGLPEGTERRDWAVGAK
                     LVVDLREDCGLPPGTVIHTFGYPEPEIFGFLYVYADRLASLGIFVPSWFESPARTSYR
                     YLQHWMLHPYLWRYLQGGRLRSWGAKTLQEAGKRGEPYLAGDGYARIGEGSGSTNVLT
                     GSGVDEAWTTGVQLAEGVIALLQEGKPFTKENLEQAYVRRRRESWVEAEGKVAERARH
                     GFHRGVLIGLVEMALTGFTRGKLTLFGKSLPPPRRVLSLEEYFRGKIPAADIAAIRRE
                     CETKGVALHDALFSRAGWPEIPYDGQLLVSQQDALLLGGKVQAPPGYPDHVVFLYPAV
                     CEACNTKICIEICSGQAITPGERGVPAFDREKCVHCGACLWNCSEPRPGDSGRTNIDF
                     RAGAGGLHSAEN"
     gene            1440..2252
                     /locus_tag="AC16G0_12650"
     CDS             1440..2252
                     /locus_tag="AC16G0_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HIR5100498.1"
                     /translation="MSNAYQIVVCGGIVPDPLQTLEPVTGPSGPALKNELMLPAVLDP
                     WASHALYEAAHLAKSAPGSKVWLLSLGPKAKLQQVMMTVAQKVPFEFVPLDGPAGGFT
                     EASEVAVALADAIQAIPGLDRSRLLVFGGWESASRGAGATMQMVGERLGILDQFHGVD
                     QLTLGENGSFRILERIEGGKHQVSTCVGPPAVLGWATGNLPEPPNNPQVGMLNMRTVM
                     PALQRSKPAKVTAAGVTFSSVSLPAQRRETRVVKDLSPDEIAREIVAWIGGA"
BASE COUNT          434 a          749 c          919 g          431 t
ORIGIN      
        1 tttccatcgg ccagttcaat cagtgggtcg gcgggcagct catgtcctcc gggacggtgc
       61 agatctggcc cgggacaccg gtgtccaagc cgctcatcga ggatggtcgt gtcgtggggg
      121 tgcggctgat cgatcagggg accgatctca aggggaatcc cgaggccggc ttcatgccag
      181 ggatggatat ccgtgctgcc ctgaccgttg tcggtgacgg gccggtcggc gccatcggtc
      241 ggcagctcga cgagcagttc ggcctcccgg aggggacgga gcgacgggac tgggcggtgg
      301 gcgccaagct cgtggttgac ctgagggagg attgcggcct cccgcccggc accgtgatcc
      361 acaccttcgg ctatcccgag ccagagatct tcggcttcct gtatgtgtat gccgatcgcc
      421 tcgcctctct tgggatcttc gtgccgtcgt ggttcgagag cccggcgcgc acctcgtacc
      481 ggtacctgca acactggatg ctgcacccgt acctctggcg atacctccag ggcggtcggc
      541 tccggtcctg gggggccaag acgctccagg aagccgggaa acgaggggag ccctacctcg
      601 cgggagatgg gtacgcccgg atcggggaag gctcgggcag caccaacgtc ctcaccggat
      661 cgggcgtgga cgaggcctgg accacggggg tgcaattggc cgagggggtc atcgccctcc
      721 tgcaagaggg aaagcccttc accaaggaga acctggaaca ggcgtacgtc aggcgtcgcc
      781 gggagagctg ggtggaggcg gagggaaagg tcgcggagcg cgcccggcat ggctttcacc
      841 ggggggtgct gatcggtctc gtggagatgg ccctcaccgg gtttacccgc gggaagttga
      901 ccctcttcgg caagtcgctt ccaccgccaa ggcgagtcct gagtctggag gagtatttcc
      961 ggggaaagat cccggctgca gacatcgcgg cgatacgccg cgagtgcgag acgaagggcg
     1021 tcgcgctcca cgacgcactc ttctcccgcg ccggctggcc ggagatcccg tacgacggcc
     1081 agctactcgt ctcgcagcag gatgccctgc tgctgggcgg caaggtgcag gccccgccgg
     1141 ggtatcccga tcacgtcgtc tttctctatc ccgcggtgtg cgaggcatgc aataccaaga
     1201 tctgcatcga gatctgctcg ggccaggcga tcacgccggg cgagcggggg gtgcccgcct
     1261 ttgaccggga aaagtgcgtc cactgcgggg cctgcctgtg gaactgttcc gagccccgcc
     1321 cgggggattc cggacggacc aatatcgact tccgggcggg ggccgggggg ctgcactcgg
     1381 cggaaaattg agaacgttcg gccgcacgag aacggccgga cgcgttggga gtaagaatga
     1441 tgagtaatgc gtatcagatc gtcgtctgcg gtggaatcgt gcccgatcca ctgcaaaccc
     1501 tggagccggt cacgggtccg agcggcccgg cgctcaagaa cgagctgatg ctgccggcag
     1561 tcctggatcc gtgggcctcg catgccttgt acgaggccgc gcacctggcg aagtcggcgc
     1621 cgggcagcaa ggtgtggctg ctgagtctgg gccccaaggc caagctccag caggtgatga
     1681 tgacggtggc ccagaaggtc ccctttgagt tcgtgccgtt ggatgggccg gcgggtggat
     1741 tcaccgaagc gtccgaagtg gcggtggcct tggccgacgc catccaggcg attccgggtt
     1801 tggatcgctc gagactgttg gtgttcggcg gatgggagtc ggcctcccgg ggggcggggg
     1861 ccacgatgca gatggttgga gagcgcctgg ggatcctgga ccagtttcat ggggtggatc
     1921 agctcaccct cggcgagaac ggcagcttcc gcatcctgga gcgcatcgag ggtggaaaac
     1981 accaagtctc aacctgcgtt ggaccacccg ccgtgctggg gtgggcgacc ggcaatctcc
     2041 ccgagccgcc gaacaacccg caggtcggca tgctcaacat gcgcacggtg atgcccgccc
     2101 tccagcggtc caagcccgcg aaggtgaccg cggccggcgt gaccttctcc agcgtgtcgc
     2161 tgcccgcaca gcggcgggag acccgagtgg tgaaggatct ctctcctgac gagatcgctc
     2221 gtgagatcgt tgcgtggatc gggggagcgt gaagactcaa aatgtggaag gggaaacaga
     2281 acaggaatca gtaaggtttt gggctgtagg tatcggcagc ttcatcgtcg atggagcaac
     2341 agtcttccag gcagagcagc gaatagggag acgcgacgga tatggagagc gtgctgttcc
     2401 ttggacacac cgaatccgat ggcaccctgg ggaaggcggc tctggaggcg ctggcggccg
     2461 cgagactcct ggccgatggg ctggggggaa agagcttcac cgccggtctt gtgggcggcc
     2521 aggtgcaggc tgc
//
LOCUS       DBNGZC010000738         4375 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1039512, whole genome shotgun sequence.
ACCESSION   DBNGZC010000738 DBNGZC010000000
VERSION     DBNGZC010000738.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1039512"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="AC16G0_12655"
     CDS             complement(<1..805)
                     /locus_tag="AC16G0_12655"
                     /inference="COORDINATES: protein motif:HMM:NF024772.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5100499.1"
                     /translation="MRRRTSRSVVAALAMLMVLGLPGPTAAGSSATTQLRVNVFPGVS
                     NLALYAAQAEGFFARRGLDIQIQFTPNSPAQREALARGDFEIAHAGVDNAIALVELAK
                     VDAIVVMGGDSGMNSLFVQPEIQSYADLRGKTVIVDAPNTAYALVLYKMLGLNGLDRG
                     AYTVKPIGGTKLRLEAMLKDKSHAATMLNPPFSVLAEHGGLRSMGLAVRTLGAYQGSG
                     MFVLRAWAQANGDNLVRYIQAYVEGLRWALSPVNKSEAAALLADRLKLSA"
     gene            complement(950..2284)
                     /locus_tag="AC16G0_12660"
     CDS             complement(950..2284)
                     /locus_tag="AC16G0_12660"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR5100500.1"
                     /translation="MGIKTFGAMAVDWEERVNFDRLRSERLARVKSLLRASEMGALLC
                     FDMTNVRYITATHIGTWAMDKLVRFSLLPQGGEPILWDFGSAARHHQLYCPWLGDRSR
                     AGISLLRGALSPESGQADDVARKIRIALEERGLLGEPVGVDVIELPVLFALQRQGIRV
                     VDGQQLMQEVRKIKTQDEITLLTSACMMVDAAYDELYRAMRPGMRENECVALVNRVLY
                     DLGSEFVEGVNAISGERCSPHPHVYTDRVLRPGDPAYFDILHSFNGYRTCYYRTFAIG
                     SASRAMVDAYKRCRDYLDAAISLIRPNVTTADVVKVWPRAQDFGFATEEDAFALQYGH
                     GVGLAIWEKPIFSRLVSLDHPEVIEENMVFALETFWPATDGWSAARIEEQLVVTKTGC
                     EIITRFPAEDLLVAGHRYYTATGPLPTTRETQSNQNRPLVEPVESPAVVGAS"
     gene            complement(2408..3301)
                     /locus_tag="AC16G0_12665"
     CDS             complement(2408..3301)
                     /locus_tag="AC16G0_12665"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5100501.1"
                     /translation="MITLGFVGLGVMGGRIAKRLLDSGHPLTGYNRTKSKAQWLLKAG
                     MKWGETPRAIAQEVDVVFTMVTNTRALQDVVGGPDGLLAGLGPGKIFVDMSTVGPSAS
                     RELASQVAARGAQMLDAPVSGSVSTLEEGKLSIMVGGDRSAFERVNPILQDIGPKVTH
                     VGGNGLAVSMKIATNLSLAVQMLAFSEGVLLAEKSGIPRKTAVEVLLNSVIASPMLKY
                     RGPFVLEMPTEAWFNVNMMQKDMTLALEMGRQLNVPLPTAATTNQFLTAARGMGVAEQ
                     DFAVIFEVLARMAGVSGSRAG"
     gene            complement(3795..3995)
                     /locus_tag="AC16G0_12670"
     CDS             complement(3795..3995)
                     /locus_tag="AC16G0_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100502.1"
                     /translation="MVGSAVYFPFKASFCILGGVSSGFALLFAGARAAEKVAGTTCRG
                     TWAITPNVVKGKETVHFAGTPH"
BASE COUNT          820 a         1462 c         1312 g          781 t
ORIGIN      
        1 ctgcgctgag ctttagccga tccgccagca gcgccgccgc ctcggacttg ttgaccggcg
       61 acagagccca ccgcagacct tcaacatatg cctgaatgta gcggaccaag ttatcgccgt
      121 ttgcctgcgc ccacgcccgc agaacgaaca tcccggaacc ctgatatgcc cccaaggtcc
      181 gcaccgcaag ccccatgctt cgtagacccc catgttcggc gagcaccgag aagggaggat
      241 tcagcatggt cgctgcatgg ctcttgtcct tgagcatggc ttccaagcgg agctttgttc
      301 ccccgatagg cttcacggtg taggccccgc ggtcgagccc gttgagaccc agcatcttgt
      361 agagcaccag ggcgtaggcg gtattgggtg cgtccacgat cacggtcttg ccacgcaggt
      421 cggcatagga ctggatttcg ggctggacga agagactgtt catcccgcta tccccgccca
      481 tgacgacgat ggcatcaacc ttcgccagct ccaccaaggc gatggcgttg tccacccccg
      541 cgtgcgcgat ctcgaaatcg ccccttgcca atgcctcacg ctgcgccggg gaattcggtg
      601 tgaactggat ttggatgtcc agtccccgcc gggcgaagaa tccctcggcc tgcgcggcgt
      661 agagcgccag gttgctgacg cctggaaaca cgttcacccg gagctgagtg gtcgcgctcg
      721 accccgcggc tgtcgggccc gggagcccca aaaccatcag catcgccagg gctgccacga
      781 ccgaccgtga agtcctccgc cgcatgcatc ctcctcgcct caccggagtg ttcgtcgccg
      841 ggagtgtcca agccgtcgtg atgcccacat tgtcaccccg gcgaaagccg gggtccagca
      901 tcccgcgacg cgactggatt ccggcctccg ccggaatgac ggacaaggcc tagctggccc
      961 cgactaccgc gggactctcc acgggctcca ccaagggccg attctggttc gattgggtct
     1021 cccgggtcgt cggcagaggg cccgtcgcgg tgtagtagcg atgcccggcg accagcaggt
     1081 cttccgccgg gaaccgcgtg atgatctcac agcctgtctt cgtgaccacg agctgctcct
     1141 cgatgcgggc ggcagaccat ccatccgtgg ccggccagaa ggtctccagg gcaaagacca
     1201 tgttctcctc gatgacctcg ggatggtcga gggagaccag acgactgaag atcggtttct
     1261 cccagatggc gagacccaca ccgtggccgt actggagtgc gaaggcgtcc tcctccgtcg
     1321 caaagccgaa atcctgggcc ctcggccaga ccttgacgac atcagccgtg gtgacgttcg
     1381 ggcggatcaa gctgatggcc gcatccaggt aatcccggca ccgcttgtac gcatccacca
     1441 tggcccgcga agcgctgccg atggcgaagg tgcggtagta gcacgtccgg tacccgttga
     1501 agctgtgcag gatgtcaaag taggccgggt ctccgggacg cagcacccga tccgtataga
     1561 cgtgtgggtg cggactgcag cgctcgccgg agatggcatt gacgccttcc acgaactccg
     1621 agccgaggtc gtagagcacc cggttgacca gggccacgca ctcgttctca cgcatgcccg
     1681 gacgcatggc acgatacagc tcgtcgtagg cggcatccac catcatgcag gcggaggtga
     1741 gcagagtgat ctcgtcctgc gtcttgatct tccgcacctc ttgcatgagc tgctggccgt
     1801 ccaccacccg gatcccctgg cgctgcagcg cgaagagcac cggcagctcg atcacgtcca
     1861 ccccgacggg ctcgccgagc aggccgcgct cctcgagggc gatccggatt tttcgggcca
     1921 catcatcggc ctggccggac tcgggtgaca gcgcgccgcg aaggagcgag atgcccgctc
     1981 gcgagcgatc gcccagccag gggcagtaga gctgatggtg tcgcgccgcg gagccgaagt
     2041 cccacaggat gggctctccc ccctggggga gcaggctgaa gcggaccagc ttatccatgg
     2101 cccaggtgcc gatgtgggtg gccgtgatgt agcgcacgtt ggtcatgtcg aagcacaaga
     2161 gagcgcccat ctcggaggcc cggagcagcg acttcacccg cgccagccgc tcgctgcgca
     2221 gccgatcgaa gttcacccgc tcttcccagt cgacggccat ggcgccaaag gtcttgatcc
     2281 ccatcgtccc ccctccggat cgctcctcca gcgcctcgct tgctaggagc gcgatcctga
     2341 atttgcctcg agaaaaagtc ccgcccggat caccccgacc acagggaagc agaacgcagc
     2401 atgacgatca gcccgccctg gacccgctca cgcctgccat gcgggccagc acctcgaaga
     2461 tgaccgcgaa atcctgttcg gctaccccca ttccacgagc ggcggtcagg aattgattgg
     2521 tcgtcgccgc cgtgggcaac ggcacattca gttgccggcc catctccagc gccagagtca
     2581 tgtccttctg catcatgttc acgttgaacc aggcctctgt cggcatctcc aggacaaagg
     2641 gtccacggta cttcagcatg ggcgaggcga tcacgctgtt caggagcact tcaacggcgg
     2701 tcttgcgggg gatgccgctc ttctctgcca ggagaacgcc ctcgctgaat gccagcatct
     2761 gcacggccag gctgagattg gtggcgatct tcatggacac cgccaggccg tttccaccca
     2821 cgtgcgtgac cttcggtccg atgtcttgca ggatgggatt gactctttcg aaggcgctgc
     2881 ggtctccacc caccatgatg gagagcttgc cttcttccag cgtgctcacg ctgccggaga
     2941 ccggggcgtc gagcatctgg gctcccctgg cggcgacctg ggaggcaagt tcccgactgg
     3001 cggagggacc gacggtgctc atatccacga agatcttccc tggaccgagt cccgccaaca
     3061 ggccatccgg gcccccgacc acatcctgca gggcccgagt gttcgtgacc atcgtgaaca
     3121 cgacatcaac ttcctgggca atggcgcgcg gcgtctcccc ccatttcatc ccggccttca
     3181 gcagccactg ggccttcgac ttggtgcggt tgtagccggt gagtgggtgc ccggaatcca
     3241 gcagccgctt ggcaatccga ccccccatca cccctaggcc gacaaaccct agcgtgatca
     3301 tcagacgccc tttctgccgt aaagctgtcg tactgtcgga tggagacctg cccttagagc
     3361 tgtcctcgcg gttgggtcgc aggaaacgta tagtctgata agatgacaag aggggccagt
     3421 caagcggaaa ccaatccggg gacgagacat tcgggcggaa agacgctgcc gattcagacg
     3481 cccaatccag ccaagctgtg cggcaataca gaaatccaat tatgccggat gggttggaag
     3541 cgctcgaaag tgggacagaa ggtcaggctg aatcccttcg tctaagcgga gcgagcttta
     3601 gcgcgcgttg ctcaccgcgg ctgcgtacgg gggagtggtg gccgaacccg atgaggttgc
     3661 gaaaggcgac atccccgatc tgatcgcagc taccctggac catcctgggg cagatgtcaa
     3721 aggcgacaac ccatgtcgtg accggccgga gtgcggcccg accgactcac tcgcgggacg
     3781 agccccagaa caggctaatg gggggtaccg gcgaagtgaa ctgtctcctt acctttcacg
     3841 acgttgggtg tgatggccca ggtcccgcga cacgtcgtac ccgccacctt ttcagccgcc
     3901 ctggccccgg caaaaagcag ggcaaagccg ctgctgacgc ccccaaggat gcagaaagac
     3961 gccttgaagg ggaagtagac ggccgaaccc accacgctgc cgacgcccgc gccaatctgc
     4021 gcccccgtgc tttcttgcga gtctccaacc gcaaagcttg ccgtgctctg cccttacagt
     4081 gccaatgcaa gcagtactac cgcaatacgt ttcatctgtc gcccctcatt cctttctgga
     4141 attcagccaa gtcctggcag ggccggatac tcgatagcgg ttgatacctt ttcatcctaa
     4201 tctgcggacc ggagggtttc aagacatttc cctatcgaag ggcggcgaca gcattacagc
     4261 gctgtgaggc tgcaaaccgc tctggactat tagccccggc ccctgcgtcg cgggtgctcc
     4321 gcgatcgccc agcccgggct cccggcgctg gcgttaggca cctctttaca ctaac
//
LOCUS       DBNGZC010000739         1449 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_65080, whole genome shotgun sequence.
ACCESSION   DBNGZC010000739 DBNGZC010000000
VERSION     DBNGZC010000739.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_65080"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1047)
                     /locus_tag="AC16G0_12675"
     CDS             complement(100..1047)
                     /locus_tag="AC16G0_12675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024685.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR5100503.1"
                     /translation="MGEPGEAHYALVTKAIADGRVVPFLGAGASLCGRPASAGFEQGK
                     YLPSGTELSEYLCQAYPAGEPRELLRIAQFVYVMEGSGPLYERLHTLFDADYPVTELH
                     ALLASIPGTLQAKGYPSPYLLVMTTNYDDLLERAFDAAGQAYDVVWYVADGDGRGKFR
                     HRSPGAEIRLIDKPNEYTGLSLRERNVILKIHGAVSRVNPEEDSYVITEDHYIDYLTR
                     TDIAGLIPVTLAAKVRKSHFLFMGYSLADWNMRVILQRIWGVQQLTYKSWAIQRQPRE
                     LDIECWKKRGVDILEVELAAYVAALRARLVALPLSGSPP"
BASE COUNT          250 a          485 c          457 g          257 t
ORIGIN      
        1 tgatgagctc ggtctccgtc tcccgtccga agaaatactc ccagtcctcc tcggaatacg
       61 ggaccagccc cacgtacgga cagcgtcgcg ccggctgggt cacggggggc tcccggacag
      121 cggcagggcc acgagtcgtg cgcgcagggc ggccacgtag gcggccagct ccacctccag
      181 aatgtccacg ccccgcttct tccagcactc gatgtccagc tcccgaggct gtcgctgtat
      241 ggcccacgac ttgtacgtga gctgctgtac gccccagatg cgctgcagga tcacgcgcat
      301 gttccagtcc gcgaggctgt accccatgaa caggaagtgg ctcttcctca ccttggccgc
      361 aagcgtgacg ggaatgaggc cggcgatgtc cgtccgcgtg agatagtcga tgtagtgatc
      421 ctcggtgatc acatagctat cctcctcagg gttcacgcgg ctgacggcgc cgtggatctt
      481 caggatcacg ttccgctctc tcaacgacag accggtgtac tcgttgggct tgtcgatcag
      541 ccgtatctcg gcccccggcg agcggtgacg gaacttccct cgaccgtcgc cgtccgccac
      601 gtaccacacc acgtcgtagg cctgcccagc ggcatcgaag gcgcgctcga gcagatcgtc
      661 gtagtttgtg gtcatgacga ggagatacgg gctcggatag cctttggcct gcagcgtgcc
      721 tggaatcgaa gccaagagcg cgtgcagctc ggtcacgggg tagtcggcgt cgaatagagt
      781 gtgcagccgc tcgtagagcg gacccgagcc ctccatcacg tagacgaact gcgcgatgcg
      841 gagcagctcg cggggctcgc cggccggata agcctggcag aggtactcgc tcagctccgt
      901 cccgctgggc aggtacttgc cttgctcgaa gccagcgctg gccggccgac cgcaaaggct
      961 ggcaccggcc ccgagaaagg gcacgacccg cccgtccgcg atggccttgg ttaccagcgc
     1021 gtagtgcgcc tctccgggtt cacccatgga tggttctcca cgccgtcgtg gtcacctcgc
     1081 cggggggagg gcgcgtagcc gcgattacct ccaagttcat ccgccggctc ccccagtatg
     1141 gcgagttcag cagcaggtcg atcgttcaga tgacggattc gagccacaac ttatcgaagg
     1201 tccagcggag tgtcaatttg aaatcccgcc tgcaagttga aggcgaggaa ctgctagtgt
     1261 cctacccctg acgtgcattg aggaattgtc ggccctcaac gccgcccatc cgccgcctgg
     1321 cccctacact cactcgttcg agtggggacc cggaaccgtc cagtcctgct ccgggtcatg
     1381 ggggggtgcg cagaagcggg actccctacg acccacggcg ggcgggctcc agcgccagcc
     1441 gcgccgagc
//
LOCUS       DBNGZC010000740         3690 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1434408, whole genome shotgun sequence.
ACCESSION   DBNGZC010000740 DBNGZC010000000
VERSION     DBNGZC010000740.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1434408"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="AC16G0_12680"
     CDS             complement(<1..547)
                     /locus_tag="AC16G0_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100504.1"
                     /translation="MTRSIRWLRFIQTLKIRSRDRRIIPLVYNRPQQIVWESIAEKID
                     RLEPIRLIVLKARREGISTAIESWMMALIASQDNVNALVTAHQKGPATKIFNMAKLFV
                     NASPLLSRLARIESGRISIGSSSLEVATAGTPEQARGGDLTAWHASEAAFYPFPEVLV
                     ATMQSLPHSKDIFSIGVIESTA"
     gene            complement(784..1215)
                     /locus_tag="AC16G0_12685"
     CDS             complement(784..1215)
                     /locus_tag="AC16G0_12685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100505.1"
                     /translation="MRLRKILTLALILLIPTVAMAQPWVVSQIMQNAAGATGPGTSID
                     TSRAASLTLQYIGGSGTLVVEASDGKTVAGTEIWTTLPCYPVGNSSVASSFTVSTTAG
                     MARCNVAGIALVRARISVFSSGPINVLGSASSIPAFSANGF"
     gene            complement(1230..1430)
                     /locus_tag="AC16G0_12690"
     CDS             complement(1230..1430)
                     /locus_tag="AC16G0_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100506.1"
                     /translation="MPESRTVSEEISHLMRKGPSKGPRKGKKYGQKQAVAAALEQERR
                     GKIRPNPRKGRGRRGRGRRRSR"
     gene            complement(1431..1610)
                     /locus_tag="AC16G0_12695"
     CDS             complement(1431..1610)
                     /locus_tag="AC16G0_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100507.1"
                     /translation="MSPRRTTAAEGDPEVIIIRSGYATDDQWLDAIAQAKATGRRVTA
                     IAANGTAWTPEQEDW"
     gene            complement(1802..2329)
                     /locus_tag="AC16G0_12700"
     CDS             complement(1802..2329)
                     /locus_tag="AC16G0_12700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100508.1"
                     /translation="MRRWLALALGLVVVWPVLVLAQPQGGPVVLSKRLQNAATTTGVG
                     ATLDTSNAAVAGIQVSGTFSGTVAFEGSIDGTTWAALTCFTPAGVSGTSTTTASLWRC
                     NVIGTTSIRANITARSSGSITVFAFATSTPQMVLDTPGAAGPTGPTGDTGPTGPTGPT
                     GSTGATGPTGATGAP"
     gene            complement(2322..2501)
                     /locus_tag="AC16G0_12705"
     CDS             complement(2322..2501)
                     /locus_tag="AC16G0_12705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100509.1"
                     /translation="MRLENHEGAVILMLGDRVPELGCPFTPDEARRLIEALEKHLRGL
                     ESARIIIPGTGGPHA"
     gene            complement(2557..2760)
                     /locus_tag="AC16G0_12710"
     CDS             complement(2557..2760)
                     /locus_tag="AC16G0_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100510.1"
                     /translation="MQAWLKDAEPLMGVQGPSDYLKGVALLSHAIHRSRGGKSKDTGA
                     TEELKALQKLMEPHGRSPKTAHG"
     gene            complement(2769..3179)
                     /locus_tag="AC16G0_12715"
     CDS             complement(2769..3179)
                     /locus_tag="AC16G0_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100511.1"
                     /translation="MSPLWEIAAFTILGLGILGVYLAGAKIARFLRQLREEQDHDHER
                     LAGHMSEWHRRFEERIEALMVEVRAARRLQVVEEPGVFLPTDEAVAEMERSLKLSEDH
                     SLGASGVIRYSSRPSRTSGTGSRREPSTQRVPRS"
     gene            complement(3222..>3690)
                     /locus_tag="AC16G0_12720"
     CDS             complement(3222..>3690)
                     /locus_tag="AC16G0_12720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100512.1"
                     /translation="LTWSTVDQGVAIGSAAYSSLTQRDAWLFQLPYSITVDSITYRRT
                     SGASGTAKVCVYSGDGSTKLIDVTDTAGTGNITVAVSPGVALSPGTYWIIIGCATTCT
                     HAITASNNTSPVFTAASPAPWNGTVTHSSGVCDSALGTVTGSAVKSGMFRFDQ"
BASE COUNT          635 a         1256 c         1136 g          663 t
ORIGIN      
        1 tcgccgtgga ctcgatgacc ccgatggaga agatatcctt ggagtgaggg agcgactgca
       61 tcgtggcaac gagcacttcg gggaaggggt agaaggcggc ttcactcgca tgccacgccg
      121 tgagatcacc accccgcgcc tgttctggcg ttcccgccgt ggcgacctcc agtgacgaac
      181 tgccgatgct gatacgaccc gactcgatgc gggcgagacg gctcaggagc ggcgaggcgt
      241 tcacgaagag cttcgccatg ttgaaaatct tggtagcggg gcccttctgg tgcgcggtga
      301 ccagggcgtt gacgttatcc tgcgaggcga tcaacgccat catccacgac tcgatggcgg
      361 tggaaatccc ctcgcgcctg gccttcagca cgatcaggcg aatgggctcc agccggtcga
      421 tcttctcggc gatggactcc cagacgattt gctgcgggcg gttgtagaca agggggatga
      481 tccggcgatc acgcgaccgg atcttcaggg tctggatgaa gcggagccag cggatcgaac
      541 gggtcactca cagggcctca acacaccatc cttcactaag aacccgagcc atgggccgaa
      601 aagatcatga gcgagtttct cggtaacgaa ccccggcacc ggagcgggat gatcctgaaa
      661 ggaccacggc ctcttatact gccgtggctc catcacctga tacatcatcg gcccgtcctt
      721 ccacgaccac gcacgatcag gacaatctgc ggcggtcatg cttggcacaa ccgccgtccc
      781 gaatcagaag ccgttcgccg agaacgccgg aatgctgctc gcgctgccga gtacgttgat
      841 ggggcccgaa ctgaagacgc tgatcctcgc tctcacgagc gcgatccccg ccacgttgca
      901 ccgcgccata cccgccgtag tcgaaaccgt aaaggacgag gccaccgagg agttacccac
      961 cggatagcac ggtaaagtag tccatatctc cgtacccgcc acggtcttcc cgtccgaggc
     1021 ttccacgacg agcgtccccg aaccgcctat gtactggagc gtgaggctgg ccgcccgcga
     1081 cgtgtcgatc gacgtccccg ggccggtggc gcccgcggcg ttctgcatga tctgggagac
     1141 cacccacggc tgcgccatgg cgacggtggg gatgagcagg atgagggcca acgtgaggat
     1201 cttccgtaga cgcatgaaag ggtctccttt taccgggagc gacggcgacc gcgaccccgc
     1261 cgaccgcgcc ccttcctggg attgggccga atcttgcccc gtcgttcctg ttccagcgcc
     1321 gccgccaccg cctgcttctg cccgtacttc ttgcccttgc gcgggccttt cgaggggccc
     1381 ttccgcatca agtggctgat ctcctccgac accgttctcg attcaggcat ctaccagtcc
     1441 tcctgctcgg gcgtccaggc cgtcccgttc gcggcaatgg ccgtcacccg ccgccccgtg
     1501 gccttggcct gcgcgatggc atcgagccat tggtcatccg tcgcgtagcc cgaccggatg
     1561 atgatgacct cggggtcacc ctccgcggcg gtcgttcttc gcggactcac tctgactcgc
     1621 ccgcccctgt ttctggcccc tcgcgccatt gaccgtggtc gagggggagt acacgggagt
     1681 ctcgatgcaa tcctcgccca agggactcac cagatgcgtg aactgatggg cgctccggcg
     1741 tcccggcctg agattgcccc acttgccgtt gtcagagaag gattcagcca tgtcggtctc
     1801 cttatggcgc ccccgtggct ccggtcggac ccgtcgcgcc agttgacccg gtgggaccag
     1861 tgggtccggt ggggccggtg tcccccgtgg ggccagtcgg ccctgccgcc cccggcgtat
     1921 caaggaccat ctgtggggta ctcgtggcga aggcgaacac ggtaatagac ccagaactcc
     1981 gagccgtgat gttggctcga atcgacgtgg tgccgatcac gttgcagcgc cagaggctcg
     2041 cggtcgtggt cgaagtgccg ctgacgcctg ccggggtgaa acacgtcagc gccgcccagg
     2101 tcgtcccgtc gatggacccc tcgaacgcca ccgtccccga gaacgtcccc gagacctgaa
     2161 tccccgccac cgccgcattc gaggtatcga gcgtagcccc gactcccgtc gtggtggcag
     2221 cgttttgcag ccgcttgctc aggaccacgg ggccgccttg aggttgcgcc agaacgagaa
     2281 ccggccacac gaccaccagg ccaagcgcca gagccagcca tctacgcatg agggcctcct
     2341 gttccgggaa tgatgatccg cgccgattcc aggccgcgca gatgcttctc tagcgcctcg
     2401 atcaaccgcc gcgcctcgtc gggcgtgaac ggacacccga gctccggtac gcggtcgccg
     2461 agcatcagga tcaccgcgcc ctcgtggttc tccagccgca cgtcatggac gagtcgcacc
     2521 cgcttgaatc gaggggtgac gacgtgcccg ttcgcctcag ccgtgcgccg tcttcgggct
     2581 tcgcccatgg ggttccatca atttctggag cgccttcaat tcctcggtcg cccccgtgtc
     2641 cttcgacttg ccgcctcggc tccgatggat ggcatgggag aggagcgcca cgcccttcag
     2701 gtaatcgctc ggtccctgca cccccatgag cggctcggcg tccttcagcc aggcttgcat
     2761 gatgcgctct aggagcgcgg gactcgctgc gtcgaaggtt cccttctcga tcccgtgccc
     2821 gaggtccgcg agggtctgga cgaatagcga atgacgcccg aggcgccgag gctgtgatcc
     2881 tcggacagct tcagcgaccg ctccatctcc gctaccgcct cgtccgtcgg gagaaacacc
     2941 cccggctcct ccaccacctg aagcctcctg gccgctctga cctccaccat caacgcctcg
     3001 atccgctcct cgaatcggcg atgccactcg ctcatgtggc cggcgaggcg ctcgtggtcg
     3061 tggtcctgtt cctctcggag ttgacggagg aaccgggcga tcttggctcc cgccaagtac
     3121 acgcccaaga tccccaagcc caagatcgtg aaggcggcga tctcccacaa aggactcatc
     3181 ttccatgaga tagcacgacc accgggtata acgtcaagcg tttactggtc aaatcggaac
     3241 atcccggact tcacggcgct gcccgtcacg gtgcccaagg cgctgtcgca caccccggaa
     3301 ctgtgtgtga ccgtgccgtt ccatggggca ggagacgccg cggtgaagac tggagacgtg
     3361 ttgttcgatg ccgtgatggc gtgcgtgcag gtcgtcgcgc agccaatgat gatccagtag
     3421 gtgccgggac tcagggccac gccaggggac accgccaccg tgatattgcc agtgccagcc
     3481 gtgtccgtca cgtcgatcag cttggtcgag ccgtcgccgg aatagacgca gaccttggcg
     3541 gtgcccgagg ccccgctggt gcgcctgtag gtgatcgaat ccacggtgat gctgtagggc
     3601 aactggaaca gccaggcgtc ccgctgcgtc agggacgaat aggcggcgct gccgatcgcc
     3661 acgccctgat cgaccgtgct ccatgtcagg
//
LOCUS       DBNGZC010000741         2026 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_946119, whole genome shotgun sequence.
ACCESSION   DBNGZC010000741 DBNGZC010000000
VERSION     DBNGZC010000741.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_946119"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="AC16G0_12725"
     CDS             <1..302
                     /locus_tag="AC16G0_12725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100513.1"
                     /translation="VKWMLSPENDSKFSQTTGALPANQSVYEEAWFKGNPFLGVFKQQ
                     LDDPAGSFNFPIQMAKWNSLIAGPLKEEFQKGLLKQQTAEATGEAIAKAISDALK"
     gene            346..1239
                     /locus_tag="AC16G0_12730"
     CDS             346..1239
                     /locus_tag="AC16G0_12730"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5100514.1"
                     /translation="MRTHLRIPSRLVPYLLLAPALGFIVLFILYPLALNVYTSFQHRV
                     FTEPTGNRFVGVANYLSVAKDPTFWLVLRQSAIWVVGSLAFQFLLGLALALLLNERFP
                     ARGLYRSLILAPWAISGVIIAIMWKWMYNPQVGVLNELLVRLGLVSHRLAFLADPGTA
                     LPAVMLTNIWRGTPFFAVMLLAALQAVPNTLYEAAAMDGAGTGQRFLHVTLPSILPMI
                     FAALLLRTIWIFNDVDLIFVMTDGGPVRASETLATFLYKKASRDLDFGMGSALAMVMF
                     AILMTITAFYYRAFRRSDRIL"
     gene            1239..>2026
                     /locus_tag="AC16G0_12735"
     CDS             1239..>2026
                     /locus_tag="AC16G0_12735"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5100515.1"
                     /translation="MPHRKPWRLVSLYLPLAGFLAFTLFPIYWTLVTSLQSEAAVARL
                     PLHLIPRELTLENYRALWGFTSFDRFFLNSLLVAGTTTLLTLAVTTLSGYALSRFRFK
                     GKTVIFFAILAIQMIPLVMIVIPLFEIFIALGLARSRLALVGTYTTFMITFCTLLMKW
                     FMDAIPAEIDEMAMIDGCSRLGALFRIVLPTIKPGLVATGIFAFIYGWNELIFAVMFL
                     NSEELMTLPVGLYSLVGQFVIKWTDIATGSIIAVLPPLLLFAYI"
BASE COUNT          304 a          701 c          590 g          431 t
ORIGIN      
        1 tggtgaagtg gatgctgagc ccggagaacg actccaagtt ctcccagacc accggagcgc
       61 tgcccgccaa ccagagcgtg tatgaggagg cctggttcaa gggcaacccg ttcctgggtg
      121 tattcaagca gcagctcgac gacccggcgg gctcattcaa cttcccgatc cagatggcca
      181 agtggaacag cctgatcgcg ggtccgctca aggaggagtt tcaaaagggg ctcctcaagc
      241 agcagaccgc cgaggcgacg ggcgaggcca tcgccaaggc tatttcggat gccctgaaat
      301 aggcggctgc ggcccccggc gggcccgggg gccgcacctt catccatgcg gacccatctg
      361 cgtatcccct cgcgtctcgt gccgtacctc ctgctcgccc cggcactcgg cttcatcgtc
      421 ctcttcatcc tctacccgct cgcgctgaat gtctacacca gcttccagca ccgcgtcttc
      481 accgagccaa ctggcaaccg gttcgtcggg gtggccaatt acctcagcgt ggccaaggat
      541 ccgaccttct ggctggtgct gcgacagtcc gccatctggg tggtgggctc gctcgccttc
      601 cagtttctcc tcggcctggc cctggccctg ctcctgaacg agcggttccc ggcgcggggg
      661 ctctaccggt ccttgatcct cgccccctgg gccatctcgg gggtgatcat cgccatcatg
      721 tggaagtgga tgtacaaccc tcaggtgggg gtcctgaacg agcttctggt gcgcctcggc
      781 ctggtaagcc accgcctggc gttcctggca gatccgggca ccgccctgcc tgcagtcatg
      841 ctcaccaaca tctggcgcgg aaccccattc tttgccgtga tgctcctggc cgccctccag
      901 gctgtgccca acaccctcta cgaggcggct gccatggacg gggccgggac cgggcagcgc
      961 ttcctccacg tcacgctgcc ctcgatcctg cccatgatct tcgcggcgct cctcctgcgc
     1021 acgatctgga tcttcaacga cgtggacctg atctttgtga tgaccgacgg cggacccgtg
     1081 cgggcctcgg agacgctggc cacgttcctc tacaagaaag cctcgcgcga tctggacttc
     1141 ggcatgggct cggcgctggc catggtcatg ttcgcaatcc tcatgaccat cacggccttc
     1201 tactaccgcg ccttccggcg ctcggacagg atcctgtgat gccacacaga aagccctggc
     1261 ggctcgtcag cctgtacctc cccctggccg gcttcctggc cttcacgctt tttcccatct
     1321 actggacgct ggtgacctcg ctccagagcg aggcggccgt ggcgcgtctt cccctgcatc
     1381 tcatcccgcg ggagctgacg ctcgagaact accgggcgct ctggggcttc accagcttcg
     1441 accggttctt cctgaacagt ctgctggtgg cggggaccac cacgctgctt actctggcgg
     1501 tcacgacgct ctcgggctat gccctctcgc gcttccggtt taagggcaag accgtcatct
     1561 tcttcgcgat cctggccatc cagatgattc ccctggtcat gatcgtgatc cccctcttcg
     1621 agatcttcat cgctctgggg ctggcccgct cgcggctggc cttggtgggg acctacacca
     1681 cgttcatgat caccttctgc accttgctca tgaagtggtt catggacgcc atcccggcgg
     1741 agatcgacga gatggcgatg atcgatggct gctcgcggct gggggcgctc ttccggatcg
     1801 tcctgccgac gatcaagccg gggctggtgg ccacggggat cttcgccttc atctatggct
     1861 ggaacgagtt gatctttgcc gtgatgttcc tcaactccga ggagctgatg accttgccgg
     1921 tggggctcta ctccttggtg ggccagttcg tgatcaagtg gacggacatc gccaccggct
     1981 ccatcattgc cgtcctgcct ccgctgctcc tgttcgcgta catcca
//
LOCUS       DBNGZC010000742         1475 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_553027, whole genome shotgun sequence.
ACCESSION   DBNGZC010000742 DBNGZC010000000
VERSION     DBNGZC010000742.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_553027"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="AC16G0_12740"
     CDS             <1..574
                     /locus_tag="AC16G0_12740"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HIR5100516.1"
                     /translation="GGACTGTGVSPKRIDGILGVCKAYTTRVGEGPFPTELSDATGEH
                     IRARGQEFGATTGRPRRTGWFDAVGVRYAVRVNGLDVLAMMKLDVLDELDPILVCTGY
                     RYRGEVLTEFPNETAVLAECQPVYESVPGWKQSTVGVITEEKLPPKCHAYIRRLESLV
                     GVPLTLVSTGPRRDQTLIRANAAWKEWGLA"
     gene            741..1022
                     /gene="rpsU"
                     /locus_tag="AC16G0_12745"
     CDS             741..1022
                     /gene="rpsU"
                     /locus_tag="AC16G0_12745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR5100517.1"
                     /translation="MSFRDREDGNGSGSHGGVAPRHRPLEVTIGERGLEGALRLFKRL
                     MLKDGILRELKHRAHYEKPGDRRRRKGREAVRRRRKQQMRARQRGEQID"
     gene            1144..1443
                     /locus_tag="AC16G0_12750"
     CDS             1144..1443
                     /locus_tag="AC16G0_12750"
                     /inference="COORDINATES: protein motif:HMM:NF012305.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR5100518.1"
                     /translation="MGTRLYVGNLPFDTDETQLRTLFSDGGRQVTEVKIITDRDTGRP
                     RGFAFVEMGSQADAEAAVSSLNGKEVGGRALTVNEAREQAPRRSGGFGGGGGRRS"
BASE COUNT          268 a          449 c          532 g          226 t
ORIGIN      
        1 cggcggcgcc tgcacaggga caggcgtgag cccgaagcgc atcgacggca tcctgggagt
       61 ctgcaaggcc tacacaaccc gcgtcggcga gggaccgttc cccacggaat tgtccgacgc
      121 caccggcgag cacatccgag cccgcggaca ggagttcggc gccaccacgg ggcgcccgcg
      181 ccgcacaggc tggttcgatg ccgtgggcgt gcgctacgca gtccgcgtga acggcctgga
      241 cgttctggcc atgatgaagc tggacgtcct ggatgaactg gaccccatcc tcgtgtgcac
      301 ggggtatcgc tatcgcgggg aggttctgac cgagtttccc aacgagaccg ccgtgctggc
      361 cgagtgccag ccggtgtacg agtcggtgcc cggctggaag cagagcaccg tcggcgtcat
      421 cacggaggag aagctccccc ccaagtgcca tgcgtacatt cgtcgtctgg agtctctggt
      481 gggtgtgcct ctcactctcg tctccaccgg gccccgccgc gatcagactc tcatccgggc
      541 caatgcagcc tggaaggaat ggggcctggc ctgaggcagg gccgcgggaa caattgccct
      601 tgacatgggc gatgtaaccc cgtagcgtga cagccataac ggttcgcacg gtcgcatgat
      661 ccgggatgac gccgcgagga cccaacctag cggcgtctat tctttccggt ggccgccaag
      721 cgcgtcacca aaggagacgt atgagcttcc gagacaggga ggatggaaat ggcagcggtt
      781 ctcatggggg tgtggccccg cggcatcggc cgctggaggt gaccatcggg gagcgaggtc
      841 tggagggagc cctgcggctg ttcaagcggc tgatgctcaa ggatggaata ctgcgcgagc
      901 taaagcaccg cgcccattat gagaagcctg gcgaccgccg ccgccggaag ggccgcgaag
      961 cggtccgccg ccgccggaag cagcagatgc gcgcgcggca acggggggag cagatcgatt
     1021 agacgcggca ccccgatatc aagatcttga tccgatcgtg gcgcgagcgg agccacgacc
     1081 gggctgagtg cttcccgctc aacattcgat gacgccggct ggccggcaga gagggtaaag
     1141 gtcatgggga cgcggttgta cgtgggcaat ctgccttttg acacggacga gacgcagctt
     1201 cgcaccctgt tttccgatgg gggacgccag gtgaccgagg tgaagatcat caccgaccgg
     1261 gatacggggc ggccacgggg attcgccttc gtggagatgg ggagccaggc ggatgcggaa
     1321 gcagcggtga gtagcctgaa tgggaaggag gtcggcggcc gggcgttgac ggtgaacgaa
     1381 gctcgcgagc aggctccgcg ccgcagcggt ggcttcgggg gcggtggcgg ccgcaggtct
     1441 tagggcgggc ccaccgacct cgctccaccg ctacc
//
LOCUS       DBNGZC010000743         1849 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1136117, whole genome shotgun sequence.
ACCESSION   DBNGZC010000743 DBNGZC010000000
VERSION     DBNGZC010000743.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1136117"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1207
                     /locus_tag="AC16G0_12755"
     CDS             <1..1207
                     /locus_tag="AC16G0_12755"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR5100519.1"
                     /translation="PQTYAPALASIRPALLAAVIAILAHIVDRYVHSRAIMPWSPEIW
                     VTAGLVGWAVLTVPLGLWPGGSVAFLLNVFLKTVVIFWLLASTVNTLTRLNQVAWWLS
                     LMAVPVAMVGIHQFQSGMFLPGAAVKRVLGYDAPLTDNPNDLALLLNLLLPLSMALFL
                     GSRRLGLRIALVTFMALSVAAVILTFSRAGFITLGVLILVYIWKLLRRSRYGCVLALL
                     GLVLISIPFLPASYLNRLSTITDIQADPTGSAQARWADTEAAARYIAEHPLVGAGVGM
                     DALALNQVRGPTWKQVHDVYLEYAVDLGILGVLLFVCLLIACIRSAILAQRLSAEIPA
                     LRGFYFLAEGIQISLLAFAVAGLFHPAAYQFYFYYIAGLAVALRPVYEAEGGAVERTR
                     SGSCDRRIE"
     gene            1210..>1849
                     /locus_tag="AC16G0_12760"
     CDS             1210..>1849
                     /locus_tag="AC16G0_12760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5100520.1"
                     /translation="MATQRVAPEGPDRLRVLQFSTLFAVGGTERHVLTLAEGLDRSRF
                     ELHVGCLKRWGEFLSRMEALRVPLEEYPIHKLYGRRTLREQLRFARYLRRNRIQIVHT
                     YNFYANVFAIPAACLAGVPVIVASIRDTGVYLADLQQRVQRATCRAAGCILVNSMAVR
                     QWLIAGGYPPHKIVVIRNGVDLSRFQAVTPHGKLRQELGLPDRGPLVAVLSRL"
BASE COUNT          281 a          575 c          586 g          407 t
ORIGIN      
        1 gcctcagacc tatgcccctg ccttggcctc catccgcccc gcgctcctgg cggcggtcat
       61 cgccatcctg gcccatatcg tcgaccggta tgtccattct cgagccatca tgccatggag
      121 cccggagatc tgggtgacgg cagggttggt cggttgggcc gtcctgacgg ttccgctggg
      181 tctgtggccg ggaggcagcg ttgcctttct cctcaacgtc ttcttgaaaa cggtcgtgat
      241 cttctggttg ctggccagca cggtgaatac gctcacgagg ctcaaccagg ttgcctggtg
      301 gctcagcctg atggccgtac ccgtggcgat ggttggcatc caccaatttc aatcggggat
      361 gttccttccc ggagcggccg tgaagcgcgt tctcggctac gacgcccccc tgacggataa
      421 cccgaatgat ttggcgctgc tcctgaacct cctgctcccc ttgagcatgg cgctcttcct
      481 ggggagccgg aggctgggcc tgcgcatcgc cctcgtgacc ttcatggcgc tcagtgtcgc
      541 ggcggtgatc ttaaccttct cgcgggcagg tttcattacc ctgggggtcc ttatcctggt
      601 ctatatctgg aagctcctca ggcggtcgcg gtatggttgc gtcctggccc tgctggggct
      661 ggtgctgatc tccattccct tccttccagc gagctacctg aaccgcctgt caaccatcac
      721 cgacatccag gctgatccga caggctcggc ccaggcgcgg tgggccgata ccgaggccgc
      781 cgcgcggtac atcgccgaac accctctggt cggagccggg gttgggatgg atgccctggc
      841 cctcaaccag gttcggggcc cgacctggaa gcaagtccat gatgtgtatc tcgagtatgc
      901 cgtcgatctg gggattctcg gggtcctatt gttcgtgtgc ctcttgatcg cctgcatccg
      961 atcggcgatc ttggcgcaac ggctgtcggc ggagatcccc gcccttcggg gattctattt
     1021 cctggccgag gggatccaga tcagcctgct ggcgttcgcc gtggccggtc tgtttcatcc
     1081 ggcggcctat caattctact tttactacat tgcggggctg gcggttgctc tccgcccggt
     1141 gtacgaagct gagggcggag ctgtggaacg aactcggagt gggtcctgcg atcggcggat
     1201 cgagtgatga tggcgacaca acgggttgca ccggaggggc cggaccggct ccgggtatta
     1261 cagttctcca ccctctttgc cgtcggtggg accgaaaggc atgtcctgac cctggcggag
     1321 ggccttgatc gctcacgatt cgaattgcac gtcgggtgcc tgaaacgttg gggagagttt
     1381 ctcagccgga tggaggcgct ccgggtcccg ctggaggaat acccgatcca taagttgtac
     1441 ggccggagaa ccctgcgaga gcagctgcga tttgcccgat accttcgacg caatcggatc
     1501 cagatcgtcc acacatataa cttttacgcc aatgtcttcg ccatcccggc ggcctgcctg
     1561 gcgggagtcc cagtcatcgt cgcctcgatc cgggataccg gggtctatct ggcagatctg
     1621 caacagcgcg tgcagagggc cacgtgccga gcggcgggat gcatcctggt caactcgatg
     1681 gcggtcaggc agtggttgat cgcggggggc tatcctccac acaagatcgt cgtgatccga
     1741 aacggggtcg acctttcgag gttccaggcc gtgacccctc atgggaagtt gcggcaggaa
     1801 ctcggcctgc cggaccgcgg cccgctggtg gcggtccttt cccgcctga
//
LOCUS       DBNGZC010000744         1335 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_65760, whole genome shotgun sequence.
ACCESSION   DBNGZC010000744 DBNGZC010000000
VERSION     DBNGZC010000744.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1335)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1335)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1335
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_65760"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="AC16G0_12765"
     CDS             complement(<1..705)
                     /locus_tag="AC16G0_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protease-associated domain-containing protein"
                     /protein_id="HIR5100521.1"
                     /translation="MKLSKAEQALAGQVSAERLWEYTLNIARKVRLSGSPDEAESFRY
                     IARTLKGWGFEIEEYRPECWTSWPGAALVEILGDAPESIPGITHAMAVSTPSDGVVLD
                     LANAGPADVIALERQPVHGRAAVCEGLAMPAKVYRVEAAGAAAQIHICGEHLHEMITS
                     VVWGSPTPETAHLLPKTPIVSVTKQGGAKLHEAMHRGRARLRIRAQVDTVWRALPVLI
                     AHLRAPKSNGDFLLFSG"
     gene            1014..1148
                     /locus_tag="AC16G0_12770"
     CDS             1014..1148
                     /locus_tag="AC16G0_12770"
                     /inference="COORDINATES: protein motif:HMM:NF017770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HIR5100522.1"
                     /translation="MAGVKCPKCSLIGPLFGIQSVNLLLTIVTLGVYSFRGTIGAICG
                     "
BASE COUNT          227 a          416 c          434 g          258 t
ORIGIN      
        1 gccgctgaag agcaggaaat caccattgct cttcggggcg cgcaggtggg cgatcaggac
       61 cggcagggcg cgccagaccg tatccacctg ggcccggata cggaggcggg cccggccgcg
      121 gtgcatcgcc tcgtgaagct tggctccacc ctgcttggtc acagacacga tgggcgtctt
      181 cggcagcagg tgggcggtct caggtgtggg tgatccccag acgacggagg tgatcatctc
      241 gtgcaggtgc tcaccacaga tgtggatctg ggccgcggca ccggctgcct cgacccggta
      301 caccttggcc ggcatggcga gaccctcgca cacggcggcg cgtccgtgca cgggctgccg
      361 ctcgagtgcg atcacgtccg ccggacccgc attcgccaga tcgaggacca ccccatccga
      421 gggcgtggag accgccatgg cgtgggtgat gcctggaatg ctctcagggg cgtcgcccag
      481 gatctccacg agagccgccc cgggccaact cgtccagcac tcgggccggt actcttcgat
      541 ctcgaatccc catcccttca gggtgcgtgc gatgtatcga aaggactcgg cttcgtccgg
      601 cgagccggac aggcggacct tccgggcgat gttcagcgtg tattcccaca gccgctccgc
      661 cgatacctgt cccgccagtg cctgctcagc ctttgaaagt ttcatgtctt cctctccgcg
      721 tagtgtcgtt gcgtccaggc ggtgcgattc gcctggagac cagtcaacta ttggaggacc
      781 ggattctgcc cgcggtgact cgcccgatga atgatgcttc attcaccggg aggatttcaa
      841 ctcagttctg caaggatcgc ttctcagcag ctcggcctgc tggccatttt tcgatgcaat
      901 actgtctggg ccccaacggc tcacgccctt cagtggccgg acgactgctc gggtgaccgc
      961 gaaggcggcc ttcagctcgc agcataggca cgagacagga agaataggcg atgatggcgg
     1021 gtgtgaagtg cccgaagtgc agtctgatag ggcccctctt tggcatccag agcgtgaacc
     1081 tgctcctgac cattgtcacc ctgggagtgt actccttcag gggtacaatt ggagcgatat
     1141 gcggatgatt tgccggacgg caaagcctcc ctgatgaccg cagggcggaa cctcgcaaga
     1201 cgtgcacgct gtcgtggcga tctcaggctg cggggggagt tctgtctact gactctggtg
     1261 ggatttcgtt gagcagaccc tggaggtcga gccgccgcgt gaacatggct agcctgccat
     1321 cggatgtgcg cggcc
//
LOCUS       DBNGZC010000745         4810 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_455934, whole genome shotgun sequence.
ACCESSION   DBNGZC010000745 DBNGZC010000000
VERSION     DBNGZC010000745.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_455934"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="AC16G0_12775"
     CDS             <1..1010
                     /locus_tag="AC16G0_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5100523.1"
                     /translation="EGRTFFVPENTEGSLSQWVYLHRQAAGLGTETAAEAVALYLPLA
                     ASRGTLGVLALRPDQRDVMQAPEQMRQLETFSSQVALALERAQLAAEAQQAQLRVETE
                     SMRSSLLSSVSHDLRTPLSTITGTASALLEAEATLDTSTRRGLLETLCQEAGRLNRLV
                     QNLLDMTRVESRSIQVRKEWHSLEEIVGTARARVESLLQNHTLTVRLPDNLGLVPLDN
                     LLIQQVLINLLENAARHTPPGTAIHLTAAEGAGEVTVEVADTGSGIAPGEEALIFGKF
                     YRGTGAAPGGAGLGLAICQGIIEAHGGRIWVESRADKGAVFRFTLPLTGSPPEMEHGD
                     E"
     gene            997..1734
                     /locus_tag="AC16G0_12780"
     CDS             997..1734
                     /locus_tag="AC16G0_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5100524.1"
                     /translation="MAMSDLHAVPDSDSTSPLPVILLVEDDAQIRKFLRATLAGQNFR
                     LIEAATAAEGIDAAVRRLPDLVILDLGLPDFDGLEVIRRLREWSRTPIIVLSAREKER
                     DKIQALDAGADDYVSKPFGLGELLARVRVALRHTVQPPGEAAEILYTVGDLSIDLARR
                     QVTVGGQPIHLTPVEFRLLAALARHPGKIVTQQQLLDEVWGPSRTEQSHYLRVYAGRL
                     RRKLEADPTNPRYLLTEPGVGYRLAAE"
     gene            1785..4481
                     /locus_tag="AC16G0_12785"
     CDS             1785..4481
                     /locus_tag="AC16G0_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018292311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HIR5100525.1"
                     /translation="MAIAGDQRPDSDALLARLKEDETRQARGRLKVFLGAAAGVGKTY
                     AMLEAARARRAEGVDVLVGWVDTHGRPETEVLLNGLEVLARRKMAYRNTSLQEFDLDA
                     TLVRRPALVLVDELAHTNVPGSRHAKRWQDVLELLDAGINVYTTVNVQHLESLNDVVA
                     KITGVQVRETVPDSVLEQADEVELIDLPPEELLQRLKDGKVYVPEQAKEAVQHFFRKG
                     NLIALRELALRRTADRVDAQMRLYMRDHAIRETWPVTERLMACIGPSPHSVRVVRAAK
                     RFADSLRAEWIVAYVETQAHARLPEADRARVTDTLRLAEQLGAETVVLSGPRVSEEIL
                     KHARVRNASKIVVGKPIRPVWKRLFSGSIADTLIRGSEDTDVYVISGEEAAHLPFVAP
                     ARPPRRQWRPWCWATAIVAASTAVAWLMFHHFDLSNLIMVYLLGVVAVASRYGRGPSA
                     LACLLSVAAFDFFFVPPYLTFAVHDTQHFITFGVMLVVALVISQLTVRIRWQAETARQ
                     RERRTAALYALSRELAGTRGVPNILQAAARHMAEVFRGQIVVFLPDASGHLVAQTDLP
                     GRFDVDTSELGTVQWVYAHQQVAGLGTATLPGAKALYLPLSASGGTLGVLGVRPENPN
                     SLDAPEHLHQLETFANQTALALERARLADEAQQAQLRVEAERLRSSLLSSVSHDLRTP
                     LASITGAASSLLEARTPLDAPTHRELLETIQEESERLSRLVNNLLQMTRLESGALQLR
                     KDWHPLEEVVGAALGRLKGPLENRPVATRLPEDLPLVPIDDVLIEQVLINLLDNAVKY
                     TPAGSPIEVAAWAEEQSVTVEVADFGPGLAIGEEGQVFEKFYRGRPAKGRGAGLGLAI
                     CRGFVEAHGGRIWAENRPEGGAIFRFSIPLVGTPPTLEPSDD"
     gene            4474..>4810
                     /locus_tag="AC16G0_12790"
     CDS             4474..>4810
                     /locus_tag="AC16G0_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5100526.1"
                     /translation="MTDTPHDELTAPAPQETAPTTGPAIVRLEDEPQIRRCLRPALPG
                     QGYRLFEAATGEAGLVEAATRQPDLIILDLGLPDMDGFEVIRRLREWTAIPIIILSAR
                     GQERDKIAAL"
BASE COUNT          804 a         1625 c         1589 g          792 t
ORIGIN      
        1 gagaggggcg cacgttcttc gtgcccgaga acacggaagg ctcgctgagc caatgggtct
       61 acctccaccg ccaagccgca ggcctcggca ccgagacggc ggcggaggcc gtcgcgctct
      121 accttccact ggccgcctcg cgcgggaccc tcggggttct ggcgctccgt cccgatcagc
      181 gcgatgtgat gcaggcaccg gagcagatgc gccagcttga gaccttcagc agccaagtgg
      241 cgctcgccct cgagagggcg cagctcgcag ccgaggcgca gcaggcgcag ctccgtgtgg
      301 agaccgaatc catgcgcagc tcgctgctca gctccgtctc gcatgacctg cgcacgccac
      361 tgtccacgat cacgggcacc gcgagcgccc tgctggaggc cgaagccacg ctggacacgt
      421 cgacgcgtcg aggacttctg gagacgctgt gccaagaggc ggggcgcctg aaccggctcg
      481 ttcagaacct gctggacatg acgcgggtcg agtcgcgatc catccaggtg cggaaggaat
      541 ggcactcgct tgaggagatt gtaggcacag cccgggctcg cgtggagtcc ttgctgcaga
      601 atcacactct aaccgtgcgg ctcccggaca acttgggcct ggtcccgctg gacaaccttc
      661 tcatccagca ggttctcatc aatctgctcg agaatgccgc ccggcacacg ccccccggga
      721 ctgcgatcca tctcacggca gccgagggag ccggggaggt caccgtcgag gtcgcggaca
      781 ccggctctgg aatcgcccct ggcgaggagg cgctgatctt cggcaagttc tatcgaggga
      841 cgggggccgc tccaggaggg gccgggctcg ggctggcaat ctgccagggc atcatcgagg
      901 cgcacggtgg tcgcatctgg gtcgagagcc gggcggacaa gggcgcggtt tttcgcttca
      961 cgctgcccct gacggggtca ccgccggaga tggagcatgg cgatgagtga cctccatgcc
     1021 gtgccagatt ccgactcgac cagccccttg ccggttatcc tcctcgtcga ggacgacgcc
     1081 cagatccgga agtttctccg cgccacactg gccgggcaga acttccgcct catcgaggcc
     1141 gccaccgccg cagagggcat cgacgcggcg gtgaggcggc tgccggatct cgtcatcctc
     1201 gacctggggc tgccggactt tgacggactg gaagtcatcc gtcgcttgcg ggagtggagc
     1261 cgcaccccga tcatcgtcct ctccgcacga gagaaagagc gcgataagat tcaagccctg
     1321 gacgcgggtg cggacgacta tgtgagcaag cccttcggcc tgggagagct tctcgcgcgg
     1381 gtgcgcgtcg cgctccgcca cacggtgcaa ccccccggcg aggccgcgga gatcctctac
     1441 accgtgggcg acctcagcat cgatttggcg aggcggcagg taactgtcgg cgggcagccg
     1501 atccacctga cacccgtcga gttccgcctg ttagccgcac tggcccgcca tccgggcaag
     1561 atcgtgaccc agcagcagct cctcgacgag gtgtggggac cgagccggac cgagcagagt
     1621 cactatctcc gggtttacgc cggccgactc cgccgaaagc tcgaggctga cccgaccaac
     1681 ccccgctacc ttctgaccga gccgggtgtc gggtatcgcc tcgccgccga atgattcatt
     1741 gtgttagaat cccgcccgtc ggaaacccac agggggctac acgcatggcg atcgcgggcg
     1801 atcagcgccc tgattctgac gccctcctgg ctcgcctcaa ggaggacgag acccgccagg
     1861 cacgcggccg gctcaaggta ttcctgggtg cggccgcggg tgtggggaag acctatgcca
     1921 tgctggaggc cgcccgtgcg cgccgcgccg agggtgtcga tgtcctggtg ggttgggtgg
     1981 atacacacgg tcgccccgag acagaggtcc tgctgaatgg gctggaggtg ctcgcccgac
     2041 gcaaaatggc ctaccggaat acgagcctgc aggaattcga cctggatgca accctcgtcc
     2101 gccgtcccgc cctcgtcctg gtggacgaac tggcccacac gaatgtcccc ggatcgcgac
     2161 atgccaagcg ctggcaggac gtcctcgagc tgctggacgc cggcatcaac gtctacacca
     2221 ccgtgaacgt tcagcacctg gaaagcttga acgacgtggt cgccaagatc acgggcgtcc
     2281 aggtccggga gacggttccc gactccgtgc tggagcaggc ggacgaggtg gagctgatcg
     2341 acctgccgcc cgaggaactg ctgcagcggc tcaaagacgg gaaggtctac gtccccgagc
     2401 aggccaagga agcggtccag catttcttcc gaaagggaaa cttgattgcg ctgcgcgaac
     2461 tggctctgcg gcgcacggcg gatcgcgtgg atgcccagat gcgtctctac atgcgcgacc
     2521 acgccatccg cgagacatgg ccggtcaccg agcggctcat ggcctgcatc ggtcccagcc
     2581 cccactcggt gcgcgtggtc cgggcggcca agcgctttgc agattctttg cgcgccgagt
     2641 ggattgtggc gtatgtcgag acgcaggcac acgcgcgcct gcccgaggcg gaccgcgccc
     2701 gcgtcaccga caccctccgg ctggcggagc aactcggagc ggagacggtc gtcctgagcg
     2761 ggccgcgcgt cagcgaggaa attctcaagc atgctcgcgt ccggaacgcc agcaagatcg
     2821 tggtgggcaa gcccatccgt ccggtgtgga agcgactctt ctccggctcc attgccgaca
     2881 cgctcatccg gggaagcgag gataccgacg tctacgtcat cagcggagaa gaggccgccc
     2941 acctgccgtt cgtcgctccg gcgcgccctc cacggaggca gtggcgtcct tggtgctggg
     3001 ccacggccat cgtggccgcc tcaacggctg tggcctggct catgtttcac cacttcgact
     3061 tgtcgaatct gatcatggtg tacttgctcg gcgtggtggc cgtggcttcc cggtatgggc
     3121 gtggcccctc cgcccttgcc tgcctcttga gcgtggctgc ctttgacttc ttctttgttc
     3181 ccccgtacct aacattcgcc gttcacgaca ctcagcactt catcaccttc ggtgtcatgt
     3241 tggtcgtggc tctcgtcatc agccagctta ccgtccgaat ccgatggcag gccgagaccg
     3301 cacgccagcg cgagcggcga acggcagccc tctacgccct gagccgggaa ctggccggca
     3361 cccgcggtgt acccaacatc ttgcaggccg ccgcccgcca catggccgag gtgttccgag
     3421 gccagatcgt ggttttcctg ccggacgcgt cagggcacct cgtggcacaa acggaccttc
     3481 cgggacggtt cgatgtggac acctccgagc ttgggaccgt ccaatgggtg tatgcccatc
     3541 aacaggtggc gggactcggg acggcgaccc tccctggggc caaggcactc tacctgcccc
     3601 tttcggcctc tggcgggacc ctgggcgtcc tgggcgtccg gccggagaat ccaaattccc
     3661 tggacgcccc cgagcatctt catcagctcg agaccttcgc caaccagacc gcgctggccc
     3721 ttgagcgagc acgcctcgcc gacgaggccc agcaggccca gctccgggtg gaggccgagc
     3781 ggcttcggag ttccctcctg agctcggtct cccacgatct gcgcaccccg ctcgcctcga
     3841 tcacgggggc cgccagtagt ctgctggagg cccggacccc gctggatgcg ccgacgcatc
     3901 gggagcttct ggagacaatc caggaggagt ccgaacgcct gagccgcctg gtgaataacc
     3961 tgctccagat gacgaggctg gagtccggcg ccttgcagct tcggaaggat tggcaccccc
     4021 tcgaagaggt tgtgggcgcc gctctgggaa ggctcaaggg tcccctggag aatcggcccg
     4081 tggccacacg gctacccgag gacctccctc tcgttcccat cgacgacgtc ctgatcgagc
     4141 aggtactgat caacttgttg gataatgccg tgaagtacac cccggccggc agcccgatcg
     4201 aggtcgcggc atgggcggag gagcagagcg tgacggtcga ggtggcggat ttcggccctg
     4261 gcctcgcgat tggagaggaa gggcaggtct tcgagaaatt ttatcgaggc cggccggcca
     4321 aggggcgggg cgcgggactc ggcctcgcca tctgtcgggg cttcgtcgag gctcacgggg
     4381 gccggatctg ggcggagaac cgtcccgagg gtggcgcgat cttccgcttc agcattcctc
     4441 tcgtcggaac tccaccgacc ctggagccga gcgatgactg acactcctca cgacgaactc
     4501 acggcacccg cgccgcaaga gacagcgccg acgacaggtc cggcgatcgt ccggctcgag
     4561 gacgagccgc agatccgccg ctgcttgcgg cccgccctgc ccggccaagg ttatcgcctt
     4621 ttcgaggcgg ccaccggcga ggcggggctg gtcgaggcgg ccacccgcca gcccgatctg
     4681 atcatcttgg acctggggct gccggacatg gacgggttcg aggtcatccg gcgcctccgg
     4741 gagtggacgg ccatcccgat catcatcctg tcggcccggg ggcaagagcg cgacaagatc
     4801 gcggcgctgg
//
LOCUS       DBNGZC010000746         1967 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_553298, whole genome shotgun sequence.
ACCESSION   DBNGZC010000746 DBNGZC010000000
VERSION     DBNGZC010000746.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_553298"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1809
                     /locus_tag="AC16G0_12795"
     CDS             76..1809
                     /locus_tag="AC16G0_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HIR5100527.1"
                     /translation="MARKHRAAHVAKVTNTIKGVTYTSYYLRRTFRQDGTVKHETLGN
                     LSDLPIHVIDLISRSLKGETFVPASEAFRITRSLPQGHVEAVLKMIRKLALEDLIASK
                     PSRRRDLVIAMIAERLLFPSSKLANTRHWRDTTLAEELGVGDATEDQLYDAMDWLLRR
                     QKAIEDKLAERHLADGAMVLYDVSSSYYEGKTCPLAHYGHDRDGKTGCPIIVYGVLTD
                     ADGRPIAVDVYPGNTGDPTTIPDQVAKLTRRFGLTRVVLVGDRGMLTQTQIDVLKRHP
                     GLGWISALLGAVIRRLLADGLLDREHLAGVRLAEISSPEFPGERRVACYNPQLAEQRR
                     HKRQELLAATQAELEALARSVARQVERPWTAAEIGVRAGKVIGRYKMAKHFQLTIRDG
                     HLGWARKQDSIRHEGLLDGIYVIRTSEPAERLSAADSVRSYKRLSLVERAFRCLKGIN
                     LLVRPIHHCLSDRVRAHILLCLLAYYVEWHLRQAWKSLLFADEELDQDRRRRDPVAPA
                     QASASARRKKKTHQTGSGLEAQSFRTLLAHLGTRSRSTCVAAGDPSGTPFLQVSEADA
                     LQAEALRLIGM"
BASE COUNT          360 a          644 c          632 g          331 t
ORIGIN      
        1 tgactaccgt ttgtagccaa aattattccg gctcgttctt gacgcatacc gggtggcctc
       61 ctaatactgg gccgcatggc acgaaaacac cgcgccgccc atgtcgccaa agtcacgaac
      121 accatcaagg gtgtcaccta taccagttac tatctccgac gcaccttccg acaggatggc
      181 acggtcaagc acgagaccct gggaaacctc tccgacctac caatccatgt catcgatctg
      241 atcagccgct ccctcaaggg cgagaccttc gtccccgcct cggaagcctt ccgaatcacg
      301 cgttcgctcc cccaggggca cgtcgaggct gtcctgaaga tgatccgcaa gctggccctg
      361 gaggacctca tcgcctccaa gccgtcgcga cgacgcgacc tggtcatcgc catgatcgcc
      421 gaacgcctcc tgttccccag ctccaagctg gccaataccc gccactggcg cgacaccaca
      481 ctggccgagg agttgggcgt cggcgatgcc accgaggacc agctctacga cgccatggac
      541 tggctgctcc gacggcagaa ggcgatcgag gacaagttgg ccgagcgcca cctggccgac
      601 ggggcgatgg tgctctacga cgtcagcagc agctattacg agggcaagac ctgcccgctg
      661 gcgcactacg gccacgaccg ggacggcaag accggttgcc cgatcatcgt ctacggcgtc
      721 ctgaccgacg ccgatgggcg gccaatcgcc gtggacgtct accccggcaa tacgggcgac
      781 cccacgacca tccccgatca ggtcgcgaag ctcacccggc gcttcggcct gacgcgcgtg
      841 gtcctggtcg gcgatcgcgg gatgctcacc cagacccaga tcgatgtgct caaacgccat
      901 cccggcctcg gctggatttc ggccctgctg ggcgcggtga tccgccgcct gctggccgat
      961 ggcctgttgg accgcgaaca cctggcgggc gtgcgcctgg ccgagatcag ctcaccggag
     1021 ttcccgggcg aacgccgggt ggcctgctac aacccccagt tggccgagca gcgccggcac
     1081 aagcgccagg agttgctggc ggcgacgcag gccgaattgg aggccttggc cagaagcgtg
     1141 gcccggcagg tggagcggcc gtggacggcg gccgagatcg gcgtgagggc gggcaaggtg
     1201 atcggccggt acaagatggc caagcatttc caactgacga tccgtgacgg gcacctgggg
     1261 tgggcccgca agcaggattc catccggcac gaagggctcc tggatgggat ctacgtgatc
     1321 cggaccagcg agcccgccga gcgcctgagc gccgcggact cggtgcgctc gtacaagcgg
     1381 ctgagcctgg tggaacgtgc cttccgctgt ctcaagggga tcaacctgct ggtacggccg
     1441 atccaccatt gcttgagtga ccgagttcgt gctcacatct tgctatgcct gttggcctac
     1501 tatgtggagt ggcacctccg ccaggcgtgg aagtccctgt tattcgcgga tgaggagttg
     1561 gaccaggacc gacggcggcg tgacccggtg gccccggctc aggcgtcggc ctcggctcgt
     1621 cgcaagaaga agacgcacca gacgggcagc ggactggagg cgcagagctt ccgcacgttg
     1681 ttggctcacc tggggacgcg gagtcgcagc acctgtgtgg cggccggtga tcccagcggg
     1741 acaccttttc tccaggtgtc tgaggctgat gccctgcagg cggaggcgct gcgattgatc
     1801 gggatgtaga cagaaccggg aaccgggaat ggacgcaagt cgttcaacac tccagatatt
     1861 tccattcctc cctgctggaa cttcgggtta gagaggcact gggccgccct tcaggggcat
     1921 gtgggtccgg tcgtggtgga tctgttcgcc gcaacgatca ccgaaat
//
LOCUS       DBNGZC010000747         3650 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1927168, whole genome shotgun sequence.
ACCESSION   DBNGZC010000747 DBNGZC010000000
VERSION     DBNGZC010000747.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1927168"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="AC16G0_12800"
     CDS             <1..301
                     /locus_tag="AC16G0_12800"
                     /inference="COORDINATES: protein motif:HMM:NF014640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR5100528.1"
                     /translation="LAQVSRQAERLRGLRQRLASWWELSRRLRQERLKVLTGKLESLS
                     PLSILARGYSICFALPERRVLKAAGDAAIGSEVAVRLHQGELHCLVQGIRPGEGG"
     gene            302..547
                     /locus_tag="AC16G0_12805"
     CDS             302..547
                     /locus_tag="AC16G0_12805"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR5100529.1"
                     /translation="MGEPTFEEALTQLEALVAKLEAGDLPLEEALRAFEEGVRLTRLC
                     TGRLEEAERRVHLLTRTPEGTEEEVPFDLGNEGETHG"
     gene            540..1436
                     /locus_tag="AC16G0_12810"
     CDS             540..1436
                     /locus_tag="AC16G0_12810"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR5100530.1"
                     /translation="MAEPDLAGIMAERRRLVDAALERWLPPQGECPPKIHEAMRYSVF
                     AGGKRLRPLLALLACEAVGGKPEDALPVAAALEMIHTYSLIHDDLPAMDDDDYRRGRP
                     TCHTVYGEAMAILAGDALLTQAFHVLANSAGREIPAVRCARIVAEIAEAAGSLGMVGG
                     QAMDIIAEGQQIQPETLLYLHTHKTGCLIRASIRAGAIAGGAEDLHLDALTRYGELVG
                     LAFQIVDDILDIEGSSQEMGKSAGSDLRKKKATYPAVLGIEVSRRDAARLLQEAKAAL
                     HPLGDAGETLSALADFVGQRRH"
     gene            1463..3367
                     /gene="dxs"
                     /locus_tag="AC16G0_12815"
     CDS             1463..3367
                     /gene="dxs"
                     /locus_tag="AC16G0_12815"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HIR5100531.1"
                     /translation="MYLERIDSPADLKALDRSDLPHVAQEIRDLIIRTVAKTGGHLAS
                     NLGSVELTLALHYVFNSPEDKILWDVGHQAYPHKILTGRRDRFQTLRQESGISGFTRR
                     DESPHDPFGAAHASTAISAALGMAEARDRQEAKFHVVAVVGDGALTAGLAWEGLNQVG
                     HLKRDLLVVLNDNTMSISPNVGAVSAYLNRVMTGRLAVRVRRETQKLLEYVPRVGTRM
                     AELARRVEEMAKGLVTPGVLFEELGFTYVGPIDGHNLDHLLTTLGNVTRLRGPVLMHV
                     VTVKGKGYAPAEGDAVKWHGAAPFEVETGEFKKKAAPMTYTEAFAQAMIKLADEDPRI
                     VGITAAMAEGTGLEKFRRAHPTRCYDVGIAEQHAVVFAAGLATQGLRPVAAIYSTFLQ
                     RAYDQVIHDVCLQNLPVTFAVDRAGIVGDDGPTHQGLYDLAFLRTVPNLTILAPRDEN
                     DVQHCLKTALSLPGPVAIRYPRGAGLGVPIEQEARVHPLGTAELLRQGDEVVLLALGT
                     MVRPALEAAERLAARGISAAVVNARFVKPLDRTLIPDLARRVGRVITIEEHALMGGFG
                     SAVLELFEEQDLTRVQVKRLGIPDLLVEQGTQASMRGRFALSVDGIQAAAESLVKVRR
                     FDLRRSEGVA"
     gene            3364..>3650
                     /locus_tag="AC16G0_12820"
     CDS             3364..>3650
                     /locus_tag="AC16G0_12820"
                     /inference="COORDINATES: protein motif:HMM:NF013856.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR5100532.1"
                     /translation="MTAARKRVDLLLVERGFADSRQDACRLILAGKVRTAAHRIEKPG
                     MALPADSPLEVAGPAHPYVSRGGVKLSHALEVFGVDPRDRLCLDVGASTGG"
BASE COUNT          605 a         1145 c         1342 g          558 t
ORIGIN      
        1 gctggcgcag gtcagccgcc aggccgagcg cttgcggggc ctccgacagc gcctggcctc
       61 ctggtgggag ttgtcgcggc ggctgcgaca ggaacgactg aaggtattga cggggaagct
      121 ggaatcgctc agtcccctct cgatcctggc gcgcgggtac agcatctgct ttgcactacc
      181 cgagcggcgc gttctgaagg cggccgggga cgccgcgatc ggctccgagg tggcggttcg
      241 tctgcatcag ggcgagctgc actgtctggt ccaggggatc cgccctgggg agggaggcta
      301 ggtgggggag ccgacgttcg aggaggccct gacacaattg gaggccctgg tggcaaagct
      361 ggaagccgga gatctcccgc tggaggaagc cctccgggcc ttcgaggagg gggtacgtct
      421 cacccggctg tgtacgggga ggctggagga ggcggagcgc cgtgtgcacc tgctgacgcg
      481 gactccggag gggacggagg aggaggtccc cttcgacctc gggaacgagg gagagaccca
      541 tggctgaacc ggacctggcc ggaatcatgg cagagcggcg caggctcgtg gatgccgcgc
      601 tggagcgatg gcttccgcca caaggcgagt gcccccccaa gattcacgag gccatgcgct
      661 acagcgtgtt cgcgggcggc aagcgccttc gccccctgct tgccctcctc gcctgcgaag
      721 cggtgggcgg gaagcccgag gacgccctac ccgtcgccgc cgccctggag atgattcata
      781 cgtactctct gattcacgac gatctccccg ccatggacga cgacgactac cgccggggga
      841 gacccacctg ccacacggtg tacggcgagg ccatggccat cctggcgggg gatgctctcc
      901 tgacccaggc cttccacgtg ctggcgaatt ccgcggggag ggagatcccg gcggtccggt
      961 gtgcccggat cgtggcggag atcgccgagg cggcgggcag cctggggatg gtgggcggcc
     1021 aggccatgga catcatcgcc gaagggcagc agatccagcc ggagacgctc ctgtacctgc
     1081 acacgcacaa gacgggctgc ttgattcggg cctccatccg ggcgggggcc atcgcgggcg
     1141 gggccgagga cctgcacctg gatgccctga cccgctacgg cgaactggtg ggtctggcct
     1201 tccagatcgt ggacgacatc ctggacatcg aggggagcag ccaggagatg ggcaagtcgg
     1261 ccgggagtga cctccggaag aagaaggcca cctatccggc tgtcctggga atcgaggtct
     1321 cccggaggga tgccgcgcgg ctcctgcagg aggcgaaggc tgccctccac cccctgggag
     1381 atgcgggtga gaccctgagc gccctggccg acttcgtcgg ccagcgacgg cactgaggca
     1441 actcgaaggc ggaggcaaca gggtgtatct ggaaaggata gattccccgg cggacttgaa
     1501 ggcattggat cgatcggatc tgccgcatgt ggcgcaggag atccgggacc tgatcatccg
     1561 gacggttgcc aagaccggtg gccacctggc ctccaacctc ggatcggtcg agttgacgct
     1621 ggccctccac tacgtcttca attcgcctga ggacaagatc ctctgggacg tggggcacca
     1681 ggcctatccc cacaagatcc tcaccggccg ccgcgaccga tttcagacgc tccgccagga
     1741 atcggggatc agtggattca cgcgacggga cgagagcccc cacgatccct tcggggccgc
     1801 ccatgccagc acggccatct cggctgcgct tggcatggct gaggcccggg atcggcagga
     1861 ggcaaagttt cacgtcgtgg cggtggtcgg ggacggcgcc ctcaccgccg gactggcctg
     1921 ggagggactg aaccaggtcg gccatctgaa gcgcgacctc ttggtagtcc tgaacgacaa
     1981 caccatgtcc atctcgccga atgtgggggc cgtctccgcc tacctgaacc gtgtgatgac
     2041 gggcaggctg gcggtgcggg tccggcggga gacgcagaag ctcctggagt acgtgccgcg
     2101 ggtgggaacc cggatggccg agctggcccg gcgcgtggag gagatggcca agggcctggt
     2161 gacgcccggt gttctcttcg aggaactggg gttcacctac gtcggaccga tcgacgggca
     2221 taacctggac catctgctga ccacactggg caacgtgacg cgactccggg gtcccgtcct
     2281 gatgcacgtc gtcacggtca aggggaaggg gtacgcgccg gccgagggag acgccgtgaa
     2341 gtggcacggg gccgcaccct tcgaggtgga gacgggggag ttcaagaaga aggctgcccc
     2401 catgacatac actgaggcct tcgcccaggc catgatcaag ctggccgacg aagatcctcg
     2461 catcgttggg atcacggcgg ccatggccga ggggacgggg ctcgaaaagt tccggcgagc
     2521 gcaccccacg cgctgctacg atgtcggcat cgccgagcaa cacgccgtgg tcttcgccgc
     2581 gggcctggca acccaggggc tgcggccggt ggcggcgatt tactccacgt tcctgcagcg
     2641 agcctacgac caggtgatcc acgacgtgtg cctgcagaac ctcccggtga cgttcgccgt
     2701 cgaccgtgcg ggcatcgtgg gagacgacgg accgacgcac caggggctct acgatttggc
     2761 attcctccgg acagtgccca acctcacgat cctcgccccg cgggatgaga atgacgtcca
     2821 gcactgcctg aagacggccc tgtccctgcc ggggccggtc gcgatccggt atccgcgagg
     2881 ggcggggctc ggtgtgccca tcgagcagga ggccagggtg caccccctgg ggacggctga
     2941 actcctgcgc cagggggatg aggtggtcct gctcgccctg ggcacgatgg tccgaccggc
     3001 tctggaggcg gcggaacggc tggcggccag agggatctcg gccgccgtgg tcaacgcccg
     3061 cttcgtcaag ccgctggacc ggacgctgat cccggacctg gcccgaaggg tgggacgggt
     3121 aatcacgatc gaagaacacg cgctgatggg ggggttcggg tcggcggtcc tggagctctt
     3181 cgaggagcag gatctcaccc gcgtccaggt gaagcgcctc ggcattccgg accttttggt
     3241 ggagcagggg acgcaggcct ccatgcgagg gcgcttcgct ctctcggtcg atggcatcca
     3301 ggccgcggcg gagtccttgg tgaaggtgcg ccgattcgac ctgcggcgga gcgagggcgt
     3361 ggcgtgacgg ctgcccgcaa gcgggtggat ctgctcctgg tggagcgggg cttcgccgac
     3421 agccggcagg atgcgtgccg cctgatcctg gcggggaagg tccgaacggc ggcgcaccgg
     3481 atagagaagc ccgggatggc gctgcccgcg gacagccccc tggaagtcgc cgggccggcg
     3541 cacccctacg tgagccgggg tggcgtgaag ctgtctcacg ccctggaggt gttcggggtg
     3601 gatccccgcg acaggttgtg cctggacgtc ggggcctcca ccggaggatt
//
LOCUS       DBNGZC010000748         2876 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_553510, whole genome shotgun sequence.
ACCESSION   DBNGZC010000748 DBNGZC010000000
VERSION     DBNGZC010000748.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_553510"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /gene="pstA"
                     /locus_tag="AC16G0_12825"
     CDS             <1..538
                     /gene="pstA"
                     /locus_tag="AC16G0_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR5100533.1"
                     /translation="VRFLADVLNGVPSIVIGIFAYTLVVLPMRRFSAVAGGVALAIIM
                     LPIVLRTTEEMVRLVPASLREAALALGIPQWKVVLRVVLPTARAGIITGVMVAVARIA
                     GETAPLLFTAFGNRFWQHGLDQPIAALPLQIFAYAIAPYDDWHRQAWAGALVLIALIF
                     SISLVARLATRGRFSGVR"
     gene            541..1299
                     /gene="pstB"
                     /locus_tag="AC16G0_12830"
     CDS             541..1299
                     /gene="pstB"
                     /locus_tag="AC16G0_12830"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR5100534.1"
                     /translation="MADGIRVKQLQAWFGNLEVLHGIDMAISPHRVTAVIGPSGCGKS
                     TFIRCLNRMHEVVPGGLAHGQVLRNGEDLYAPEVDPVNIRRRIGMVFQRPNPFPTMSV
                     FDNVAAGLRLDGVRNRGLIQDAAERALQQAALWDEVKDQLGKSGASLSGGQQQRLCIA
                     RALAVRPEVLLMDEPCSALDPIATAKIEELIVELKRELTIVIVTHNMQQAARVSEETG
                     FFLLGELVEFDETTKIFTNPQDRRTEDYITGRFG"
     gene            1369..2034
                     /gene="phoU"
                     /locus_tag="AC16G0_12835"
     CDS             1369..2034
                     /gene="phoU"
                     /locus_tag="AC16G0_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR5100535.1"
                     /translation="MQRHFHDELNQIKEKTLKLGSLVETMVEHAVASLVDRDTRLAEE
                     TIASDARVDTLEVEIDEGCIRLLALYQPAASDLRFITTAMKISTDLERMADQAVNISQ
                     RAIDLNEEPQLKPYIDIPLMSQLSQKMLRDSLDAFVRRDVALAQEVIQADSKVDALKD
                     QVFRELLTFMMEDPRTIPRAIRLILVSRHLERLADHATNIAEMVVFLVEGKDIRHAPY
                     PKT"
     gene            complement(2182..2505)
                     /locus_tag="AC16G0_12840"
     CDS             complement(2182..2505)
                     /locus_tag="AC16G0_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100536.1"
                     /translation="MNAMSFCQSLTDRLTVIQVIRGIFARSPVFRDALISDPRLGSEG
                     EVIVDVSFDRDPAAFRVVAPSAEEAYAILHELASAMVEIERGRHGWNGNGKYGHEASS
                     PEMTG"
     gene            complement(2606..>2876)
                     /locus_tag="AC16G0_12845"
     CDS             complement(2606..>2876)
                     /locus_tag="AC16G0_12845"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HIR5100537.1"
                     /translation="LEVNTVLSGPHGGDVQIAAEVGSGTIDAVFFFVDPLTAQPHDPD
                     IQALMRLCNVHNIPRATNLATADLLIAGLPKNGQAGDRHGDKTTG"
BASE COUNT          519 a          944 c          877 g          536 t
ORIGIN      
        1 tgtccgcttt ctggctgatg tcctgaacgg cgttccatcc atcgtgatcg gcatcttcgc
       61 ctacaccttg gtggtactcc ccatgcggcg tttctccgcc gtggccggcg gcgttgcgct
      121 ggcaatcatc atgctgccca tcgtcctccg gacgacggaa gagatggtgc gcctcgtccc
      181 agcctcactc cgcgaggcgg ccctggcgct gggcatcccc caatggaagg tcgtgcttcg
      241 cgtggtcctg ccgaccgccc gtgccggcat catcaccggg gtcatggttg cggtcgcccg
      301 gatcgccggg gagacggccc ccctgctctt cacggctttc ggcaaccgct tctggcagca
      361 cggcctggat cagcccatcg cagccctgcc ccttcagatc ttcgcctatg ccatcgcccc
      421 ctacgacgac tggcaccggc aggcctgggc tggagccctg gtcctgatcg ccttgatttt
      481 ttcgataagc ctggttgccc gcctggcgac caggggccga ttcagcgggg tgagatgacc
      541 atggcggacg gcattcgagt caaacaactc caagcctggt ttggaaacct agaggttctc
      601 cacggaattg acatggcgat ttcaccccac cgggtgaccg ctgtgatcgg cccctcgggg
      661 tgcggcaagt cgaccttcat tcgttgtttg aatcggatgc acgaagtcgt tccgggcgga
      721 ctagcccacg ggcaggtcct caggaacggg gaggatttgt atgcccccga ggtcgacccg
      781 gtgaacatcc ggcgtcggat cgggatggtc ttccagcggc ctaacccctt cccgaccatg
      841 tccgtgttcg acaacgtggc ggcgggcctg cggctggacg gcgtccggaa tcgcggcctc
      901 attcaggacg cggcagagcg cgccctccag caggcggctt tgtgggacga ggtgaaggat
      961 cagttgggaa aatcgggggc gagtctctcc ggggggcagc aacagcggct ctgcatcgcc
     1021 cgcgcgctgg ccgtgaggcc agaggtcctg ttgatggatg agccctgctc ggctctggac
     1081 cccatcgcga cggccaagat cgaggaactg atcgtggagc tgaaacgaga gctgaccatc
     1141 gtcatcgtca cccataacat gcagcaggcg gcccgcgtat ccgaggagac cggctttttc
     1201 ctgctgggcg agctggtgga gttcgacgag accacgaaga tcttcaccaa cccgcaggat
     1261 cggcggaccg aagactacat cacggggcgc ttcggataga tcgtcgaccc tcccgctccg
     1321 atcccatctc acgcgtcatg agcggcgcca tgcgctggcg agggtacaat gcagcggcac
     1381 tttcacgacg agctgaacca gatcaaggag aagaccctga agctcggtag tctggtggag
     1441 accatggtcg agcatgccgt ggcctccctg gtggatcgag acactcggct ggccgaggag
     1501 accatcgcct ccgacgcgag ggtggacacc ctggaggtgg agatcgatga gggctgtatc
     1561 cggctgctgg ccctctacca gcctgcagcc agcgacctcc gcttcatcac cacggccatg
     1621 aagatcagca ccgacctgga gcggatggcc gaccaagccg tgaacatctc tcagcgggcc
     1681 atcgatctta acgaagagcc ccagctcaag ccctacatcg acattcccct catgagccag
     1741 ctctcccaga agatgctgcg agacagcctg gatgcctttg tccgccggga tgtcgcgttg
     1801 gcccaagaag tcattcaggc cgacagcaaa gtggacgccc tgaaggatca ggtcttccgg
     1861 gagctcttga ccttcatgat ggaggacccc cggacgatcc cccgcgccat ccgcctcatc
     1921 ctggtgtcgc ggcatctgga gcggctcgcc gaccacgcca ccaacatcgc cgagatggtc
     1981 gtcttcctcg tcgagggcaa ggacatccgt cacgcgccgt atccgaagac ttgactcgca
     2041 catctcgtat gagccagtcg ggaagcctcc cctcacagac tgagcgattc ggaagttggg
     2101 cgtcatgttc gccctgaccc ctttggctcg cgatgaatgc cctctcaaga gagtgtgcgt
     2161 cgctccccgc gcaccggcct gttagcctgt catctcaggc gagctggcct catgaccata
     2221 cttcccattc ccgttccagc cgtggcggcc tcgttcaatc tcgaccatgg cggaggccag
     2281 ttcgtggagg atcgcatacg cctcctccgc cgagggcgcg accacccgga aggctgcggg
     2341 atcccggtca aagctgacat ccacgatcac ctcaccttcg gatccgaggc gcggatcgga
     2401 gatgagggcg tcgcggaata ccggagaccg ggcgaagatc ccccggatga cctggatcac
     2461 cgtgagacga tccgtcaagg attggcagaa tgacattgca ttcacgtgtt ccctcccgca
     2521 atccgaatgg cccatcggag ccttcgaccg cttccccctc gccagtctcg cagcgacgcg
     2581 cgctgcgcaa cgagcaggag gcaagtcagc ctgtcgtttt gtccccatgc ctatccccag
     2641 cttggccgtt cttcggcaat cctgcgatga gcagatcggc ggtcgccaag ttggtggccc
     2701 ggggaatgtt gtgaacattg cacaggcgca tgagtgcctg gatatccgga tcgtgaggct
     2761 gcgccgtcag gggatccaca aagaaaaaca ccgcatcgat cgtcccggag ccgacctctg
     2821 ctgcgatctg cacgtccccg ccgtgggggc cgctgaggac cgtgttcacc tcgagc
//
LOCUS       DBNGZC010000749         1894 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2019993, whole genome shotgun sequence.
ACCESSION   DBNGZC010000749 DBNGZC010000000
VERSION     DBNGZC010000749.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2019993"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..1280)
                     /locus_tag="AC16G0_12850"
     CDS             complement(117..1280)
                     /locus_tag="AC16G0_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100538.1"
                     /translation="MAALGIRKGATPALQSQVGRLTVLLPSVELARAELSRQGPTLDE
                     KTVHRMARQLGAEVLATRTRDLQRYREGRLPAGQEMAGQNVVAEVDGGRVRIRTQVKT
                     TKRRGVKYRRKIRVEWREPKLLIVYLSDRQGRMLKGTRPWIDGTMSGPDHLMELLAFH
                     LHRLGAARAKAVSFVSDGAPWIWNRLDWVVGRAGLDPKRVERVLDCCHAVHHVSLALQ
                     ALGMEEAERAATYRALRHQLRAGRSREVVARLRALADGQPPDSDVWREIEFLDKHEAH
                     LRYDWFRYRRRPLGSGAVESAIRRVINLRLKGNGIYWREENAEAILVLRAAALTGRWQ
                     ETMERTQAEMAKDRCRDWQWEAPDIVGELNSGMAIKPPSPQPESAQDAEAFAA"
     gene            complement(1391..1693)
                     /locus_tag="AC16G0_12855"
     CDS             complement(1391..1693)
                     /locus_tag="AC16G0_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100539.1"
                     /translation="MEIRTESRTGPLVPPPADVTTALGRRIDAVKQQWLDQLTRDPSS
                     FGRLEVEIHEEFRRLADEMTASLLAEATASDNRAEPGKKGGLAGPTPHDVPRKSDG"
     gene            1714..>1894
                     /locus_tag="AC16G0_12860"
     CDS             1714..>1894
                     /locus_tag="AC16G0_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100540.1"
                     /translation="MCEKQRSCYGDSRIIATFWESTLVQDLLSAKRDLRLSRVFKSLS
                     RYEVLILDDLGYVQQS"
BASE COUNT          299 a          673 c          581 g          341 t
ORIGIN      
        1 gttcgcaggt tggtctaccg ccggctactc gcgagagatt cctgaccact tcctgatgaa
       61 tctctagaaa ctcctggagg cgatcgtctt tgatcgggtg gattcccgaa aagggatcat
      121 gctgcaaacg cctcagcgtc ctgtgctgat tcaggttgtg gtgacggcgg cttgatcgcc
      181 atgcccgagt tcaactcccc tacgatgtcg ggagcctccc actgccagtc gcggcagcgg
      241 tccttcgcca tctcggcctg tgtcctttcc atcgtttcct gccaccgacc cgtcaatgcc
      301 gctgcgcgca gcacgaggat cgcctcggcg ttctcctcac gccaatagat cccgttgccc
      361 ttcagacgca ggttgatcac ccggcggatc gcactctcca ctgcgccact gcccagggga
      421 cgacgccggt accggaacca gtcatagcgc aggtgggcct cgtgcttgtc caggaactcg
      481 atctcacgcc acacgtccga gtcgggcggt tggccgtcgg ccaaggccct gagcctggca
      541 accacctcgc ggctgcggcc cgcgcgcaac tggtgacgaa gggcccgata cgtcgcggcc
      601 cgctccgcct cctccatccc cagcgcctgt aacgccaggc tcacgtgatg gaccgcgtgg
      661 caacaatcca gaacccgctc gacgcgcttg ggatccagcc ccgcacgccc cacgacccaa
      721 tccaaccggt tccagatcca gggggcaccg tcggacacga acgacacggc cttggctcgg
      781 gcggccccca gacggtgcag gtggaacgcc agcagttcca tcaggtggtc cggcccgctc
      841 atcgtgccgt cgatccacgg ccgcgtcccc ttgagcattc gcccctggcg gtcgctgaga
      901 tagacgatca acagcttcgg ttcccgccac tcgacacgga tcttgcgacg gtacttcacg
      961 ccccggcgtt tcgtcgtctt gacctgcgtg cggatccgca cccgcccgcc gtccacctcg
     1021 gcgacgacgt tctgtccggc catctcctga cccgccggga gtcggccttc gcggtaccgc
     1081 tgcaagtcac gcgtccgtgt cgccaggacc tcggccccca actggcgcgc catgcggtgc
     1141 accgtcttct catcgagcgt cggcccctgg cggctcagtt cggcgcgggc caactcgacc
     1201 gaggggagca agacggtgag acggcctacc tgcgattgca gggccggggt ggctcccttg
     1261 cgaatgccca gcgccgccaa ctcgggatag gtcccgccgc cctcccgacc tcggcccttg
     1321 cccgtgcgtg acctggggcc gagataggcc gtggcgaccc agacgaccag gccacccagg
     1381 aggcggagtc tcagccgtcg ctcttgcggg ggacgtcgtg aggggtcggt cccgcaagac
     1441 cccctttttt cccgggctcg gcccggttgt ccgaggccgt tgcctccgcc agcaagctgg
     1501 cggtcatctc atccgcgagg cgacggaact cctcgtggat ctcgacctcg agccgcccga
     1561 aactgctggg atctcgcgtc aattggtcaa gccactgctg cttcacagcg tcgatccgcc
     1621 gacccaaggc ggtggtgacg tctgccgggg ggggaacaag aggaccggtt cgtgactccg
     1681 tgcggatctc catctgcgcc tccgtggaga tgggtgtgcg agaagcagag atcatgctac
     1741 ggagattcgc ggatcatcgc cactttttgg gaatcgaccc tggtacagga cctgctctcg
     1801 gccaagcggg acctgcggct cagccgggtg ttcaagagcc tctcgcggta cgaggtcctg
     1861 atcctggatg acctgggcta cgtgcagcag agcc
//
LOCUS       DBNGZC010000750         2355 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1927722, whole genome shotgun sequence.
ACCESSION   DBNGZC010000750 DBNGZC010000000
VERSION     DBNGZC010000750.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1927722"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(447..725)
                     /locus_tag="AC16G0_12865"
     CDS             complement(447..725)
                     /locus_tag="AC16G0_12865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100541.1"
                     /translation="MATIVEYTDAKFPQNLYPKRIISPSHSSPCCFSEMEEIGPVQRD
                     GHWEYRYKRCRKCGFSLRVILRALPDERLLRDLRWTLENSFKRNVPDL"
     gene            complement(1001..1234)
                     /locus_tag="AC16G0_12870"
     CDS             complement(1001..1234)
                     /locus_tag="AC16G0_12870"
                     /inference="COORDINATES: protein motif:HMM:NF038399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Os1348 family NHLP clan protein"
                     /protein_id="HIR5100542.1"
                     /translation="MCQAAVEKTLGKLVTDETFRVQFFQDPAAASFSAGLELSRAELD
                     ALSRLSVEAIVQFSAGVDDRIRRLPLDAQSRRT"
     gene            complement(1599..>2355)
                     /locus_tag="AC16G0_12875"
     CDS             complement(1599..>2355)
                     /locus_tag="AC16G0_12875"
                     /inference="COORDINATES: protein motif:HMM:NF012385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR5100543.1"
                     /translation="ESELFGHERGAFTGADKQKLGRVEMAAGGTLFLDEVAELPPSAQ
                     AKLLRVLQEHEFQRVGGTATLRADVRLIAATNQDLAHAVEAGTFREDVYYRLNVFPLH
                     LPPLRERGDDVLLLAHHFVRELGARLGKGEPGLSRDAQDALRSYPWPGNIRELENAIE
                     RALILSEGGLLTAMHLGVSGVGAQRPVGASGQAGDGNAAPESLPDLEKRAILAALEAT
                     HGNKTRAAAQLGITRTKLHTRLKRFGLVPESPV"
BASE COUNT          445 a          740 c          750 g          420 t
ORIGIN      
        1 taccaagagg agcaccggga agtccgggtt gtgacgcaag ttccccaccg ccgccggctg
       61 gaagtgcgcg agcacgttgg gatcatagat gtttgggaca ccggcaggca gggccgctgc
      121 tgtggaccct gctgacgttg ccgcgacgag gatacagctc aacagcagcg gcaagatgac
      181 tatctggcct ggaggcgtcc atcggcgctc gcggggggcc gcctggcatc gtccagagcc
      241 gggcacgagg gcaatttggt cgggggatag gctgagccct ctgaccagcc gaacgacgtc
      301 acggaaaaat gtcaggagga tggaaaaggt tcgcggcttg tttcgatctt tcatgatccc
      361 cttcccttct cgtccgcggt cgcatcacag atatctgtac gcaattccac cctgacggga
      421 ccggccgccc gtgcggacag gaaatttcac agatccggga cgttccgttt gaaggagttc
      481 tccaaagtcc agcggaggtc gcgaagcaat cgctcgtccg gaagtgcccg gaggatcacg
      541 cgcaggctga atccgcattt tcggcaacgc ttgtaccgat attcccagtg accgtcccgc
      601 tggacggggc caatttcctc catctcgctg aagcagcatg gactcgaatg cgagggagaa
      661 atgattcgct tcgggtagag gttctggggg aacttggcgt ccgtgtattc aacaatggtc
      721 gccatcgcca actcctgtgt gcgtatcggc tacgcgcgat taaggtccgc ccccagggca
      781 gaggttctga ccactggtag aatcaagatg cttgggacac gtctccacct gctatctggc
      841 acaagccgag aaagccgggc cgaggaccgc aggaaagacg gcgaaggctc gggggagatc
      901 ctcatttgtc ctcctgtgtg tctcgagcag accagcacgc ctgccctgct gtggaaccag
      961 tgtgtccccg gcgcacacca ggtgcgcccc ggccgggctg tcaggtccgt cgcgattgcg
     1021 catccaacgg gaggcggcgg atccgatcgt ccacgcccgc gctgaactgc acgatcgcct
     1081 caacagaaag tcgagacagc gcgtccaatt cggctctcga gagctcgagt cccgcagaga
     1141 agctggccgc cgccggatcc tgaaagaact gaacccggaa ggtctcatcc gtcaccaact
     1201 tccccaacgt cttttcaacc gctgcctggc acatggcgcg tctttgaatc cagcgagtcg
     1261 ctcgtacgtt agcaggccgc tgaaaaaggc ccatctgcgg cgttggcgcg ctcgggcgct
     1321 cgctgccgcg tacacgtaag tacgcctcac tcggccccgg gtacccatct ggaagatggg
     1381 tgacgccgcc ttgcatctag acctttttga gcagcctgga cgaaaatggg ttcttcagcc
     1441 tcccgttaga ggaggtatgc agcgccggtg ccaatgacct ggtcgccgac taactcatcg
     1501 gaaagacgca ccagaggagg agccttgcag gatcaagacg ggtgaggtgt acaggggtga
     1561 tcgacgcagt gcttaggggt gagcagtcgc gtgtagaatc agacgggtga ttcaggaacg
     1621 agcccgaagc gcttcaaacg ggtgtggagc ttggtccggg tgataccgag ctgggccgcc
     1681 gcgcgggtct tgttgccgtg agtggcctcg agggcagcca ggatcgcgcg cttttccaga
     1741 tcaggcaggg actcgggggc ggcgttccca tctccagcct gcccgctcgc gcctaccggc
     1801 cgttgcgccc ccaccccact gacgcccagg tgcatcgctg tgaggaggcc cccctcggag
     1861 aggatgaggg cgcgctcgat cgcgttctcc agctcccgaa tgttgccagg ccatggatat
     1921 gaacgtagcg cgtcctgggc gtcgcggctg agccctggct cccccttgcc cagacgggcg
     1981 cccaactccc gcacgaagtg gtgggccaag agcaaaacgt cgtcgccccg ctcccgcaac
     2041 gggggcaggt gcagcgggaa cacgttcagc cggtagtaga catcctcgcg aaacgtgccc
     2101 gcttctaccg cgtgcgccag atcctggttc gtggcggcga tcaggcggac gtcggcccgc
     2161 agtgtggcag tgccccccac gcgctgaaac tcgtgttcct ggagcacccg caagagcttg
     2221 gcctgggcac tcgggggaag ctcggcaacc tcatccagaa aaagtgtccc tcccgcggcc
     2281 atctcgaccc ggcccagctt ctgcttgtcg gcgccggtaa aggccccgcg ctcgtggccg
     2341 aacagctccg actcc
//
LOCUS       DBNGZC010000751         3153 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1336754, whole genome shotgun sequence.
ACCESSION   DBNGZC010000751 DBNGZC010000000
VERSION     DBNGZC010000751.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3153)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3153)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3153
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1336754"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            557..2737
                     /locus_tag="AC16G0_12880"
     CDS             557..2737
                     /locus_tag="AC16G0_12880"
                     /inference="COORDINATES: protein motif:HMM:NF012464.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR5100544.1"
                     /translation="MNSLDATKHADRPDLAGPALHPSISQPRAFRSAKILDWHLDRLA
                     IVYVRQSDPHQVLNHRESRERQYGLADHAAALGWTRDRVLIIDDDQGVSARTAGVRGG
                     FQRLLAEVTMEHVGLIVGIEMSRIARSNKDWHNLLEMCAIFGTLLADEDGVYDPLDTN
                     DRLLLGLKGTISEFELVTMRNRLERGRLHKAQRGELFYRVPTGYIKLSSDRIEMDPDE
                     QVRDVVRLIFDKYDEIGSVWGVFHYLIRNHIRLGFRPFHGPHRGNLEWRRPVLVTVFE
                     ILRNPMYAGAYAYGRRTCQRARTAPEEHSGAGKWVPMDQWKVLKHDCLPAYITWAEYL
                     AHQECLRQHRSGFGSKGSPRDGSALLGGLLVCGTCGRRLQPTYRSPDRAYYNCVRYLH
                     EATEQVCFGVKATVIDDLVTQQVLRALEPAALELSCRALEDVQRERARLGKHWKQRLQ
                     RARYEAEDAERRYRAVDPENRLVARSLEQRWEETLGAERQVRDDYDRFLREQPPQLSQ
                     QERSRIAALSSDLPALWHDPGTTHRDRKEIIRHLVEKVVVHGKSDSEYVDVTIHWQGG
                     FLSQHEVLRPVRSYEQLRDFDQLMDRITTLRRDGKTTVQIADALNREGFSTPRRCGAF
                     TAEIVNALLQRRGLANEKTHTNQLGPHEWWLPKLADAIPVSAGKLGDWARRGWIHARR
                     TPAQRLWVLWADEPEANRLHKLAALFHRGMAEYPSELTTPRDRR"
     gene            complement(2802..3038)
                     /locus_tag="AC16G0_12885"
     CDS             complement(2802..3038)
                     /locus_tag="AC16G0_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100545.1"
                     /translation="MYAKLYRHKDEFVVIDDVDALYADRSGVRLLKCLCQTEEEKTIA
                     RHSDARSLERQRIPREFTTKSRVAIISTDWKTQT"
BASE COUNT          580 a         1046 c          975 g          552 t
ORIGIN      
        1 gaagaatttc agcggggttg gggccccagg ccgctgccgc agaaaattcg gggcctgctg
       61 gatcaagtgg tcaagatggt ggcctgacta ccctctgttc tcgcagagtg cagggtagat
      121 ctccgcatcg tcaaaccacc tgccggcttc acgatgcacc tcaagggcgc caggctcaaa
      181 gtggagcaca tgcccgcgat cctggagggc cgccacgttc ttgttgaggg tctgccagaa
      241 gacacaaacg gactgcacct gaagccgtgc gtcgtggacc gccatcgtcg ccgcccataa
      301 tcggcgtacc ggcgttgctg agcctggcgg attggatcgc cacacgccgg gtctcagtcc
      361 accaaggagg tccctcatgc cacaccaccg agcccgctca gtcccccccc gcgcccaccg
      421 gagccactcc agcgcctccc gttgactcga ctctggcaag ctctccccca agccgatcgc
      481 cagcgggcgc tgctgacgct cagccgcatc gtcgctcagc acctgcccag gcctcccgcg
      541 ggcaaggagg ccggccatga actcgcttga tgcaaccaag cacgccgacc gacccgatct
      601 cgccgggccg gctctccacc cctctatcag tcaaccccga gccttccgat ctgccaagat
      661 cctcgattgg cacctcgatc gcctggccat cgtctacgtg cgccaatcgg atccccacca
      721 ggtcctcaac caccgcgaat cgcgggaacg gcaatatggc cttgccgacc atgccgccgc
      781 cttgggatgg acccgagacc gcgtcctgat catcgatgac gatcaggggg tcagcgctcg
      841 cacggccggc gtgcgaggtg gatttcaacg ccttctcgcc gaagttacca tggagcacgt
      901 cggtttgatc gtcgggatcg agatgagccg catcgcccgc agcaacaaag actggcataa
      961 tctcctggag atgtgtgcca tcttcgggac gctgctcgcg gacgaggacg gtgtctatga
     1021 tcccttggat accaacgaca ggctcctgct cgggttgaag gggacgatca gcgaattcga
     1081 gcttgttact atgagaaatc gactggagcg tggccgactc cacaaggctc agcgcggcga
     1141 gctcttttac cgcgtgccca ccggctacat caagctctcc tcggatcgaa tcgagatgga
     1201 ccccgatgag caggtccgcg atgtcgtccg cctgatcttc gacaagtacg acgagatcgg
     1261 cagtgtgtgg ggcgtcttcc actacctgat ccgcaaccac atccgactcg ggtttcgtcc
     1321 gttccacgga ccccatcggg gcaacctgga atggcggcgc ccggtcctgg tcaccgtctt
     1381 cgagatcctg agaaacccga tgtacgcggg ggcctacgcc tatggccgcc gcacgtgcca
     1441 gcgcgcccgg acggccccgg aggaacacag tggcgccggc aagtgggtcc ccatggacca
     1501 gtggaaggtc ctgaagcacg attgcctgcc agcctacatc acgtgggctg agtaccttgc
     1561 gcaccaagaa tgcttgcgcc aacaccgatc cggttttggc tccaagggaa gcccccgtga
     1621 tggctcggcg ctgctcggtg ggttgctggt ctgcggcacc tgcggtcggc gtctccagcc
     1681 cacttaccgg tccccagacc gggcctatta caattgcgtc cggtatctcc atgaggcgac
     1741 cgaacaggtc tgcttcggcg tcaaggccac ggtgatcgac gacctggtga cgcagcaagt
     1801 ccttcgcgcc ctggagcccg ccgcgctgga attgagctgc cgggccctgg aggatgtcca
     1861 acgggagcgc gcccgactgg gcaagcattg gaaacagcgg ctccagcgcg cccggtacga
     1921 ggccgaagac gccgaacgac gttaccgggc cgtcgacccc gaaaaccgcc tggtggcccg
     1981 atccctggag cagcggtggg aggagacgct gggggccgaa cgtcaggtcc gtgacgatta
     2041 cgatcggttt ctccgcgaac agccgccgca gctctcacag caggagcggt ctcgaattgc
     2101 ggccctctcg tccgacctcc ccgcgctctg gcacgacccc ggcacgaccc accgggatcg
     2161 caaggagatc atccggcacc tggtggaaaa ggtcgtggtc cacggcaagt ccgatagcga
     2221 atatgtggat gtcacgatcc attggcaagg cggcttcctc agccaacacg aggtcctgcg
     2281 acccgtccgt tcgtacgagc aactccgcga cttcgaccag ctcatggacc gcatcacgac
     2341 cttgcggcgc gacggaaaga ccacggtgca gatcgccgac gccttgaacc gggaggggtt
     2401 cagcacgccg aggcggtgtg gggcgttcac tgccgaaatc gtcaacgcgt tgctgcagcg
     2461 tcgcggcctg gccaatgaga agacgcatac caaccagctc gggccgcacg agtggtggct
     2521 gccgaagctc gccgacgcga tcccggtgtc cgccggcaaa cttggcgact gggcgcgccg
     2581 ggggtggatc cacgcccgga ggacgcccgc tcaacgcctc tgggtcctct gggccgacga
     2641 gccggaggcg aaccggctcc acaagctcgc ggccctgttc caccgcggaa tggcggagta
     2701 cccgtccgaa ctcaccacgc cgagggatcg gcgttgacgt ccttcccgtt gatcctcggg
     2761 aaggaagact cgagggtcga cggcgcttaa caaggggcgc attatgtctg cgtcttccag
     2821 tcggtggaga tgatcgcgac gcgactcttg gtcgtgaact cgcgcgggat gcgctggcgc
     2881 tcgaggctcc gggcatcgga gtgccgggcg atcgtctttt cttcttccgt ctggcaaaga
     2941 cacttgagga gccgcacgcc ggatctgtcg gcgtagagcg catccacgtc gtctattacg
     3001 acaaactcgt ctttatgccg gtagagcttg gcgtacatgc cacttttaga ccccgcgaga
     3061 agatcgagga cgcagtggaa ggcttgttct gatggccagc ccgagatcta cggtttggga
     3121 ccgatgtccg gtttctgctc acgggtttgc ttc
//
LOCUS       DBNGZC010000752         2557 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1827790, whole genome shotgun sequence.
ACCESSION   DBNGZC010000752 DBNGZC010000000
VERSION     DBNGZC010000752.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1827790"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="AC16G0_12890"
     CDS             complement(<1..770)
                     /locus_tag="AC16G0_12890"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR5100546.1"
                     /translation="MTTHRVRAATLVAPGRYELREYPLPDPAPGCVLVKMELSGVCGT
                     DKHAYQGYITQYAGTGASRQLPLPIIQGHENVGTIAAIGGDGRYTDFDGIPLKAGDRV
                     VVGANVVCGTCYYCRHDFPYFCCENMVDYGNNMSAATPPHLFGGWSEYIYIVPGSFLV
                     RVPDNLPSEVAVLTEVMAVAVGLDRAKQFSAVPNESFLFDDTVVVLGVGPLGMCFLMK
                     ARMLGAGTIIAIDLSAYRLEMAKRLGADHTLNAAGTSVA"
     gene            complement(783..1895)
                     /locus_tag="AC16G0_12895"
     CDS             complement(783..1895)
                     /locus_tag="AC16G0_12895"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR5100547.1"
                     /translation="MRITAIRTRQVDIPLPAPFYPAWAPGRVETQIRVAYLCIDTDAG
                     ITGIAGHEFYGAEEQCVQRVATYLVGEDPLRIEKHAGTLRYLWPYFGTAVWFVEVALW
                     DILGKVAGLPLYRLLGNAHDALPAYASTGQNRTPAERAEDARRLQDEGFRAVKLRIHN
                     ETPADDLAQVRAVRKAVGDGMAIMVDANQTDVHDAPLPGPHWSYHRALETAQGLAELG
                     VTWLEEPLPRHDYGNLRRLRERSPIPIAGGEVNQGFADLQRLLLDGCYDILQPDVTLC
                     EGFLRLRALATMAQTASVLVNPHTWGDPLGMVANLHLAAAIPNTSYFEFPHDPPAFPA
                     GVYQQTLKQPLAIKDGMVQLPQEPGLGVELQDWIFA"
     gene            complement(1947..>2557)
                     /locus_tag="AC16G0_12900"
     CDS             complement(1947..>2557)
                     /locus_tag="AC16G0_12900"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5100548.1"
                     /translation="SPATNEALDYARKLFAEMEPTVLSWDDSGNNKYILSGRGSWTIN
                     APSIYLKAKKDKMPFAEAIQQNPPPAGPKGRFYYADIHGWGVLKFGQNVDLSKQLLRS
                     MYTEESQKLFLTLGQGYDMPVLQRFDVNPPYATDPQLRPLVGYMKNAHLAAYPAAPDV
                     RAEKAYQTYIIPNMFARAVQSSSNRDAIAFAVRALGDIGYSK"
BASE COUNT          428 a          813 c          877 g          439 t
ORIGIN      
        1 gccacgctgg tcccggctgc attcagggtg tgatccgcgc ccaggcgctt ggccatctcc
       61 agacgatagg ccgacaggtc gatggcaatg atggtcccgg cgccgagcat ccgggctttc
      121 atcaggaaac acatcccgag ggggccgacc cccagcacca ccacggtgtc gtcgaagaga
      181 aaagactcgt tggggacggc cgagaattgc ttggcccgat ccaggccgac ggccaccgcc
      241 atgacctcgg tcaacaccgc gacctccgag ggcaggttgt ctggcacccg caccaggaag
      301 ctccccggca cgatatagat gtactccgac cagccgccaa agagatgggg cggggtggcg
      361 gcgctcatgt tgttgccgta gtccaccatg ttctcgcaac agaagtaagg aaagtcgtgc
      421 cggcagtagt aacaggtgcc gcataccacg ttggcgccga cgacgacacg atcgcccgcc
      481 ttgagcggga tgccgtcgaa gtcggtgtat cgcccgtctc ctccgatggc ggcgatggtg
      541 cccacgttct catgcccctg gatgattggg agcgggagct gcctggaagc ccccgtgccc
      601 gcgtactggg tgatgtagcc ctggtaggcg tgcttgtcgg taccgcagac ccccgacagc
      661 tccatcttca ccagaacgca tcccggcgcc ggatcgggca gggggtactc gcggagctcg
      721 tagcgtcccg gcgcgaccag ggtggccgcg cgcacgcgat gggtggtcat atcgagtgct
      781 cctcaggcaa agatccagtc ctggagctcg accccgaggc cgggctcctg gggcagctgc
      841 accattccat ccttgatcgc cagcggctgc ttcagcgtct gctggtagac gccggcgggg
      901 aaggccggag ggtcatgggg aaactcgaag tacgacgtgt tggggatggc cgccgccaga
      961 tgcaggttgg ccaccatccc tagcgggtct ccccaggtat ggggattcac caggacggag
     1021 gcagtctgag ccatggtggc gagcgcccgc aggcgcagaa atccctcgca gagggtcacg
     1081 tccggctgga ggatgtcgta acagccgtcc aggagcagcc gctggagatc ggcaaaaccc
     1141 tggttcacct cgccgccggc gatggggatc ggcgaacgct cacgcagccg ccggaggttg
     1201 ccgtaatcgt gtcgcggaag cggctcttcc agccacgtca cgccgagttc cgccaggccc
     1261 tgggccgtct ccagcgcccg atggtagctc cagtggggtc cggggagggg cgcgtcgtgg
     1321 acgtcggtct ggttggcatc caccatgatg gccatgccgt cgcccacggc cttgcggacg
     1381 gccctgacct gcgccaggtc gtcggccggc gtctcgttgt ggatgcgcag cttgacggcc
     1441 cgaaacccct catcctgaag ccggcgagca tcctcggcgc gctccgcggg ggtccggttc
     1501 tgacccgtgg aggcgtaggc tggcaaggcg tcgtgggcat tgcccagcag ccggtagagc
     1561 ggaagaccgg cgaccttgcc caggatgtcc cagagggcca cctccacgaa ccacacggcg
     1621 gttccgaagt agggccagag gtagcgaagg gtgccggcgt gcttctcgat ccgcagggga
     1681 tcttccccca cgagataggt ggcgacccgc tggacgcact gctcctcggc gccgtagaac
     1741 tcgtgccccg cgatccccgt gatgcccgca tcggtgtcga tgcagaggta ggcaacccga
     1801 atctgcgtct ccacccgtcc cggcgcccag gcggggtaga acggagccgg cagggggata
     1861 tccacctgcc gggtccggat ggcggtgatg cgcatgctgt ccacctcgga cggaggccgg
     1921 gggccacagg gcccccggcg cgaaggtcac ttgctgtatc cgatgtcgcc caaggctcgc
     1981 acggcgaagg cgatggcatc tctgttcgag ctgctctgca cggcccgggc gaacatgttg
     2041 gggatgatgt acgtctggta cgccttctcg gctcggacat ccggcgccgc ggggtatgcg
     2101 gccaggtggg cgttcttcat gtacccgacc agaggccgca actgcggatc cgtagcgtag
     2161 ggcgggttca cgtcgaaacg ttgcagcacc ggcatgtcgt agccctggcc cagggtgagg
     2221 aagagcttct ggctctcctc ggtgtacatg gagcgcagca gttgcttcga cagatccaca
     2281 ttctggccga acttcagcac gccccacccg tggatgtctg cgtagtagaa gcggcccttg
     2341 gggccggcgg gcggcgggtt ctgctggatg gcctcggcga agggcatctt gtccttcttg
     2401 gccttgagat agatgctggg tgcattgatg gtccaggacc cgcggcccga gaggatgtac
     2461 ttgttattgc cgctgtcgtc ccaggagagg accgtgggct ccatctcagc aaacagcttg
     2521 cgagcgtaat ccagcgcctc attcgttgcc gggctgt
//
LOCUS       DBNGZC010000753         5024 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1827797, whole genome shotgun sequence.
ACCESSION   DBNGZC010000753 DBNGZC010000000
VERSION     DBNGZC010000753.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5024)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1827797"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1406
                     /locus_tag="AC16G0_12905"
     CDS             <1..1406
                     /locus_tag="AC16G0_12905"
                     /inference="COORDINATES: protein motif:HMM:NF014516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5100549.1"
                     /translation="QAELDLLAIEKDLAAKRAEEARLSQQLELFGIELQTVEADLARI
                     VGDVEGLRVVIAEGEARAGEVGQTLAAHEGEVTGLRERRETLATRLAEQRVTLASRTA
                     NRDETLRDLARMRHDLTVAEEETQRLTQESADLSLRCASLEATQAELRARLTSLHQEE
                     AARQATLQAAQEARATAQEAIQALEEEFRTKRREEAEASQQIAAMEARRGELKMAITH
                     LEQDLLQDHALGLPELRERFAETTLELEAARTELDELHAKLAEMGPTNLAALEEYEAL
                     CQRHEFLTSQAADLTQSVASLRQAILEVNKTINTLFTTTLQAVNDQFDHFWRRLIGGG
                     SAQLKLVDPPDEGEEQTEMEPGVEMLVRIPGKRATVLSLLSGGERALAALSLLLALFA
                     VRPSPFCVLDEVDAPLDDSNVERFVGLLREMSQTSQFIVVTHNKRTMEAASILYGITM
                     EEEGVSKMISVRMKAAA"
     gene            1540..2475
                     /gene="ftsY"
                     /locus_tag="AC16G0_12910"
     CDS             1540..2475
                     /gene="ftsY"
                     /locus_tag="AC16G0_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR5100550.1"
                     /translation="MAPESPPAPSGFFSRLKDGLRKTRDSLRSHLEGLFTGAPLDEAA
                     LDSLEEALLASDLGPQTTAAILEPARNIFLRQGATTAEALRALLADRIADLLRIEGPP
                     DAQPMAPPHVTLFVGTNGSGKTTSIAKLAKRYQDAGERVLLAAGDTFRAAAIEQLQLW
                     GQRIGAEVVAHRPGADPAAVVFDACKAARSRGVGRLLIDTAGRLHTKRNLMEELKKIH
                     RVIGREIPDAPHATFLVLDATTGLNALAQAREFHQAVALTGLIVTKLDGTAKGGAVVA
                     IAHSLQLPVRYVGVGESPDDLQPFNPREFATALFA"
     gene            2657..3784
                     /gene="ribD"
                     /locus_tag="AC16G0_12915"
     CDS             2657..3784
                     /gene="ribD"
                     /locus_tag="AC16G0_12915"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIR5100551.1"
                     /translation="MAEPNETDERWMRRALDLAARARGRTSPNPMVGAVLVKDGEVVG
                     EGFHAYAGSDHAEVVALNEAEAAAAGSTLYVGLEPCCHHGRTPPCVERIVAAGVRRVV
                     AACEDPNPAVSGKGIAALRAAGIQVDLGVLAEEATRLNEAFFTYIRTGRPFVILKAAA
                     SLDGKIATRTGESRWITGEAARHRVHQLRNEVDVVLVGIGTVLRDDPQLTTRLGITDQ
                     RDPARVIVDNLARLPLRAKVVNRASTAPTLLAVSQTAPRARLEALEREGIQVIVVDGS
                     PRRVSLAHLMEALGKLGFLSAMIEGGAEINASALREGVVDKVLLFLAPILIGGKSAPT
                     SVGGDGIESLGQATRLRDLRIERFNDDILLEGYIRKTEGTL"
     gene            3878..4528
                     /locus_tag="AC16G0_12920"
     CDS             3878..4528
                     /locus_tag="AC16G0_12920"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HIR5100552.1"
                     /translation="MFTGIIESIGTVREFSRHPHGTRTRIAAVEVAGELRAGDSIAVD
                     GACLTVSLVSGGEFGCDLSAETLERTTLGRLRSGTRVNLERSLRLGDRLGGHFVTGHV
                     DAVGHIAGRIPQGGAEFLRIGFPRVLAPLLVMKGSIAVDGISLTVAELTQDTFGVALI
                     PYTLEGTTLSQKRVGEMVNLEADLLGKYVARCLGERPTPSPSGGLTLSVLQEHGLA"
     gene            4657..>5024
                     /locus_tag="AC16G0_12925"
     CDS             4657..>5024
                     /locus_tag="AC16G0_12925"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009231 - riboflavin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HIR5100553.1"
                     /translation="MVIVVDDEDRENEGDLTIAAEKVSARAINFMAAHGRGLICMPII
                     GERLDQLQIPAMVSENTSPFETAFAVSVEAKHKVSTGISAQDRAATVKTVLDPNTRPD
                     DLARPGHMFPLRAREGGVLTR"
BASE COUNT          834 a         1701 c         1687 g          802 t
ORIGIN      
        1 ggcaggccga gctggatctg ctggcgatcg agaaggatct ggcggccaag cgcgccgagg
       61 aggcgcgcct cagtcagcag ctcgagctct tcgggatcga gcttcagacc gtggaggccg
      121 atctggcgcg catcgtcggg gatgtcgagg gtctgcgcgt cgtcatcgcc gagggcgagg
      181 cgcgtgccgg cgaggtcggt caaaccctgg ccgcccacga aggggaggtg acggggcttc
      241 gcgagcgacg cgagacgctc gccacccggc tggccgaaca gcgggtgacg ctggcctcgc
      301 ggacggcgaa ccgagacgag acgctccgcg atctcgcgcg gatgcgccac gacctcacgg
      361 tcgcggaaga ggagacccag cgtctcaccc aggaatcggc ggacctcagc ctgaggtgtg
      421 cctccttgga ggccacccag gcggagctgc gcgcgcgtct cacctccctc catcaggagg
      481 aggcggctcg ccaggcgacg ctgcaggcgg cgcaggaggc cagggcgacg gcgcaggagg
      541 ccatccaggc gttggaggag gagtttcgga ccaagcgccg ggaagaggcg gaggcaagcc
      601 agcagatcgc cgccatggag gcgcggcgcg gcgagctgaa gatggccatc acccatctgg
      661 agcaggatct cctccaggat cacgccctgg gcctgccgga gttgcgcgag cgattcgccg
      721 agacgacctt ggagctcgag gccgcccgga cggagctgga cgagctccac gccaagctcg
      781 ccgagatggg ccctaccaat ctggcagccc tggaggagta cgaggccctc tgccagcggc
      841 acgagttcct cacctcccag gccgcggacc tcacccaatc ggtggcctcg ctgcggcagg
      901 ccatcctgga ggtcaacaag accatcaaca ccctcttcac cacgacgctg caggcggtaa
      961 acgaccagtt cgaccacttc tggcgccgcc tcatcggtgg cgggagcgca caactcaagc
     1021 ttgtggaccc tccggacgag ggcgaggaac agacggagat ggagccgggc gtggagatgc
     1081 ttgtccgcat ccccggcaag cgtgccacgg ttctctcgct cctctcgggc ggcgaacgcg
     1141 ccctggcagc cctgtccctg ctcttggccc tgttcgcggt tcggcccagc cccttctgcg
     1201 tgctggatga ggtggacgcc cccctggacg actccaacgt ggagcgcttc gtcggcctgc
     1261 tccgagagat gtcccagacc tcccagttca tcgtggtcac ccacaacaag cgcaccatgg
     1321 aggcggccag cattctgtac gggatcacca tggaggagga aggcgtctcc aagatgattt
     1381 ccgtgcgcat gaaggccgcc gcataagcgt acgagccttc ggtggtcggg aacagccgag
     1441 ctgccgaacg gtcgagctgc cgaacgcctg ggagggtgtc gtggatttcc aagacctgca
     1501 atcgatcatc gcctccgtca acctgaacgg ctgccgctga tggccccaga gtctccgccc
     1561 gctccgtccg gctttttttc ccgtctgaag gatggcctgc gcaagacgcg cgacagcctc
     1621 cggagccatc tggaagggct gttcaccggc gcccctctgg atgaggctgc cctcgattcc
     1681 ctggaggagg ccctcctcgc ctccgacctc ggcccgcaaa ccacggccgc catcctggag
     1741 cccgctcgga acatcttcct gcgccaaggg gccaccacgg ccgaggcact gcgcgccttg
     1801 ctggcggacc ggatcgccga cctcctgcgc atcgagggcc ccccggatgc ccagccgatg
     1861 gcccccccgc acgtcacgct ctttgtgggg accaacggat cggggaagac caccagcatc
     1921 gccaagctcg ccaagcgata ccaggatgcc ggcgagcggg tgcttctggc cgcaggcgat
     1981 accttccgcg ctgcggcgat cgagcagctc cagttatggg gacagcggat cggcgccgag
     2041 gtcgtcgccc accgacctgg cgcggacccg gccgccgtgg tgttcgatgc ctgcaaggcg
     2101 gcaagatcgc gcggggtcgg caggctcctg attgacaccg ccggacgcct tcataccaag
     2161 cgcaacctca tggaagagct gaagaagatc caccgcgtca tcggccgaga gattccggac
     2221 gctccgcacg caacttttct ggtcttggat gccacgaccg ggctgaacgc cctggcccag
     2281 gcccgggagt tccaccaagc cgtggccctc acgggtctga tcgtcaccaa gctggatggc
     2341 accgccaaag ggggagccgt ggtggccatc gcccactccc tgcagctccc ggttcgatac
     2401 gtcggtgtgg gcgagtctcc ggacgacctt caacccttca acccgcgcga gttcgccacg
     2461 gccctctttg cctgaaacag gtattcagcc atcggccatc ggtcattggc cagagagcac
     2521 gggtgtggtc ttgctgtttt gcccgctggc cctgcgctgg aggcttctct ccgctcgggt
     2581 ttcgccttga cactccccgt ctcgcggtgc tagctttgcc tcgcaaaaat gagaaatgac
     2641 ggtaggttgc ggggccatgg ccgagccgaa cgagaccgat gagcgctgga tgcgccgcgc
     2701 gttggacctc gcggcgcgcg cccgcggacg gaccagtcca aaccccatgg tgggggcggt
     2761 cctggtcaag gacggcgagg tcgtgggcga aggcttccat gcctacgccg gctccgacca
     2821 cgccgaggtg gtggccctga acgaggccga ggcagccgcc gccggttcca cgctctatgt
     2881 gggcctggaa ccctgctgcc atcatgggcg gacgcctccg tgtgtggagc ggatcgtggc
     2941 tgccggggtc cgccgcgtgg tcgccgcgtg cgaggacccc aacccggcgg tgagcggcaa
     3001 aggcatcgca gcccttcggg cggccggcat ccaggtggac ctcggcgtcc tggccgaaga
     3061 ggcaacgcga ctcaacgagg cgttcttcac gtacatccgg acgggacggc ccttcgtgat
     3121 cttgaaggca gccgccagtc tggacggaaa gattgcgacg cgcacgggag agtcccgctg
     3181 gatcacgggc gaagccgccc ggcatcgcgt ccaccagctc cgcaacgaag tggatgttgt
     3241 tctcgtgggt atcggcacgg tcctgcggga tgatccccag ctcaccacgc gcctggggat
     3301 cacggaccag cgggaccccg cccgggtgat cgtggacaac ctggcccgtc tccccctccg
     3361 ggccaaggtg gtcaatcgcg cctccacggc accgacgttg ctcgcggtct cccagacggc
     3421 tccccgagcg aggctggagg ccttggagcg agaaggcatc caggtcatcg tcgtggacgg
     3481 gagtcctcgc cgcgtctccc tggctcatct catggaggct ctggggaagc tcggcttcct
     3541 ctcggccatg atcgaggggg gtgcggagat caacgcctcg gccctccgcg agggggtcgt
     3601 ggacaaggtg ctcttgttct tggcccctat cctgattggg gggaaatccg ccccgacgtc
     3661 cgtgggaggc gatggcatcg aatccctcgg ccaggcgaca cgattgcggg atctgcggat
     3721 cgagcgcttc aacgacgaca tcctcctgga aggatacatc cggaagaccg aaggaacgct
     3781 ctagggcgtc atccgaggag aggcgctcga ccgtccggct gttcgaagca actggtgctg
     3841 gcgaaccgtc gggctgccga actgccgaac cgttcttatg ttcacgggaa tcatcgaatc
     3901 catcggaacc gtccgggagt tctctcggca tccccatggg acgcggactc gcatcgccgc
     3961 ggtcgaggtg gccggcgaac tccgagccgg ggacagcatc gccgtggacg gggcctgttt
     4021 gaccgtcagc ctggtgtctg gtggcgaatt cggctgcgac ctctcggccg agacgctgga
     4081 gcggaccacc ttgggtcggc tccgctctgg cacgcgggtg aacctggagc ggtcgctccg
     4141 actgggggac cgcctgggcg ggcactttgt caccggccac gtggatgcgg tcggccacat
     4201 cgcaggccgg attccccagg ggggcgcgga gtttcttcgc atcggctttc cccgcgtact
     4261 ggcaccgctc ctggtcatga agggctccat cgccgtggac gggatcagcc tgacggtggc
     4321 ggagctcacg caggacacct ttggtgtggc cctcatccct tacaccttgg agggaaccac
     4381 cctcagccag aagcgcgtgg gcgagatggt gaacttggaa gccgacctcc tgggcaagta
     4441 tgtcgcccgc tgcctgggag agcgcccgac cccctcgccg agcggagggc tcaccctgtc
     4501 tgtacttcag gagcacggcc tcgcatgagc acccggacca gcccacgtcg tcgccccgat
     4561 tcgccagctc cccgtgccgg cgcgtccacc cgggccgggg cccccattcg ctttgcgcgg
     4621 atcgaggacg ccatccggga tattcgcaat gggaggatgg tcatcgtcgt ggatgacgag
     4681 gaccgggaga atgaagggga cttgaccatc gccgccgaga aggtctccgc cagggccatc
     4741 aacttcatgg cggcccacgg tcgcggcctg atctgcatgc ccatcattgg cgagcgtctg
     4801 gaccagttgc agatccccgc catggtttcg gagaatacct cgcccttcga gactgccttc
     4861 gccgtctcgg tcgaggccaa gcacaaggtc agcactggca tctcggccca ggatcgggcc
     4921 gccacggtca aaaccgtcct cgaccccaac acccgaccgg acgacctggc ccgtccgggg
     4981 cacatgttcc cgctgcgggc gcgggagggc ggggtcctca cccg
//
LOCUS       DBNGZC010000754         3044 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2610569, whole genome shotgun sequence.
ACCESSION   DBNGZC010000754 DBNGZC010000000
VERSION     DBNGZC010000754.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2610569"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="AC16G0_12930"
     CDS             complement(<1..170)
                     /locus_tag="AC16G0_12930"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017908149.1"
                     /note="Catalyzes the cycloisomerization of
                     cis,cis-muconate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HIR5100554.1"
                     /translation="MTSGAGHLLSNLFGSPTVDAIFSDQGRLQGMLDFEAALAAAEAR
                     TGVIPAAAAPAIS"
     gene            complement(246..815)
                     /gene="pcaG"
                     /locus_tag="AC16G0_12935"
     CDS             complement(246..815)
                     /gene="pcaG"
                     /locus_tag="AC16G0_12935"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003189308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HIR5100555.1"
                     /translation="MSLIPTPSQTVGPYFSLGMAWLTTTDLAKEASNGERIVIAGSVL
                     DGDGQPIPDAILEVWQANASGKYAHPEDTQDKPLDSGFSGFGRIPTDTEGRFRFVTIK
                     PGVTSGPGNTLQAPHILVAVFMRGLGKQLYTRIYFSDEPANATDPLLARIAEPARRET
                     LMASRLAGSDEYRWDIRMQRERETVFFDC"
     gene            complement(817..1521)
                     /gene="pcaH"
                     /locus_tag="AC16G0_12940"
     CDS             complement(817..1521)
                     /gene="pcaH"
                     /locus_tag="AC16G0_12940"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018610922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HIR5100556.1"
                     /translation="MGEPIGYRRPKPGTQPNYLHPPYQSSIRRAPSKPLILLPHTLSE
                     ITGPVYGHDRVRPTDSDLTQQHAGEPLGERIIVSGRVLDEDGRPVPRTLVEIWQTNAA
                     GRYHHLVDQHPAPLDPNFTGAGRALTDDHGHYRFVSIKPGAYPWRNHYNAWRPPHIHF
                     SLFGPSFLTRLVTQMYFEGDPLLRLDPIFNSVPDPSARARMVSSFDLETTIPEFAVGY
                     RFDIVLRGRNATPMES"
     gene            1914..>3044
                     /locus_tag="AC16G0_12945"
     CDS             1914..>3044
                     /locus_tag="AC16G0_12945"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR5100557.1"
                     /translation="MIEPYTLDVPRRPDPYPYYAQLREMDPAHYSQVEDIWVLTRFDD
                     CLAAFSDWQTWSSERRGNLLNDLPERIGRTLGTTDPPRHTGARRLVNTAFTPRIIAGL
                     EPLIRELSASLHDAACGKGTFEYVQDVSAPLNARILGAMFGVPEEEFLRVRGWLDDFF
                     VRDKPEPGREPRQVTAMRQLRAYVDTLVADRLCRPGDDLMSAMLRVREGDVSLSHEQV
                     AVTTMTFLTAGFESTNNLFTNMVAALAGHPDVLARLRREPAAVPAFTEEAMRWDAAAQ
                     GFVRTPTRDVELHGRVIPAGAQVLVHIGAANRDPRAFPDPDRFDLDRVSSRHLGLGQG
                     THFCIGGPLARVLARVAVEDLVARAARIQIDLRTAVRVSTPNF"
BASE COUNT          519 a          978 c         1023 g          524 t
ORIGIN      
        1 gaaatcgccg gcgccgcggc tgccgggatc acgccggtcc gcgcctcggc cgcggcgagc
       61 gccgcctcaa aatccagcat gccttggagg cgaccctggt cggagaagat ggcgtcgacc
      121 gtgggggatc cgaacaggtt gctgagcaaa tgacctgccc cggaagtcat cgccacccgt
      181 cgtcccccct gccatggcgc ccggcatgcc gacgcattcg acagccccgg cggcgagaca
      241 ctccttcagc aatcgaaaaa gaccgtctcg cgttctctct gcatgcggat gtcccagcga
      301 tattcatcgc taccggcgag gcggctcgcc atcagggtct cgcgacgcgc cggctccgcg
      361 atccgtgcca gcagcgggtc ggtggcgttg gccggctcgt cggaaaagta gatccgggtg
      421 tagagctgct tgcccagacc gcgcatgaac acggcgacga ggatgtgcgg cgcttggagc
      481 gtgttgcccg ggcccgacgt gactccaggc ttgatcgtca cgaaccgaaa ccggccttct
      541 gtatccgtcg ggatccgccc gaagccgctg aaccctgaat ccaggggttt gtcctgtgta
      601 tcctccggat gggcatactt gccggaagcg ttggcctgcc acacctcgag gatggcatcg
      661 gggatgggtt ggccgtcgcc gtcgagcacg gacccggcga tgacgatccg ctcgccgttg
      721 gatgcctcct tggcgagatc ggtggtggta agccatgcca tgccgagact gaagtagggc
      781 ccgacggtct gggagggggt gggaatcagg ctcacgtcac gactccatgg gcgtcgcatt
      841 gcgaccccgc agaacgatgt cgaagcggta accgacggcg aactccggga tcgttgtctc
      901 cagatcgaag gacgacacca tgcgggcacg cgcggatgga tcgggcacgc tgttaaagat
      961 gggatccagc cgcaacaggg gatcaccctc gaaatacatc tgggtcacca gtctggtcag
     1021 gaagctcggc ccgaacaggg aaaaatggat gtgggggggc cgccaggcgt tgtaatgatt
     1081 gcgccacgga tacgcgcccg gcttgatgct gacgaaccga tagtggccgt ggtcgtcggt
     1141 gagggctcga ccagccccgg tgaagttggg atcgagggga gccggatgtt gatccaccag
     1201 atgatggtac cgtcctgcgg cgttcgtctg ccagatctcg acgagggtgc gtggcacggg
     1261 acggccgtcc tcatccagca cgcggccgct cacgatgatc cgctcgccga gcggctcgcc
     1321 cgcatgctgc tgggtgagat cgctgtcggt gggtctcacc cgatcgtggc cgtagaccgg
     1381 tccggtgatt tcggatagcg tatgtggcaa gagaatcaac ggcttggacg gtgctcgcct
     1441 gatcgatgac tggtacggag ggtgcaagta attgggctga gtgccgggtt tcggacgccg
     1501 atagccgata ggctccccca tttcccctcc cccctgagcc agaccgtgtt ttctgtttct
     1561 tgcaccgact ggatgggttg ttgaccgtcg tacacctcac cgctgttccc ggccggcagc
     1621 gcatccgatt tctgtagctg ataaggtggt aagattctgg tgtcaatgga aaaccgcggg
     1681 tggaggcgca gaaatacggc gaaaacagcc agagggccgc gagcgggggt ggggagaggc
     1741 tggtcccgag tcggcaggct tccattgaca gccggctcct aacggtgcct aatgaagagc
     1801 cacccgaaac gaggaccagg gtggagacct ccgagctttc ccccccagtt ccagaaatag
     1861 acttgaggcg aatccagcca tcgccggcgg tatggctgtg aggaggatcg gccgtgatcg
     1921 agccctacac cctcgatgtt ccgcgccgac ccgacccgta cccgtactac gcgcagttgc
     1981 gtgagatgga cccggcccac tactcgcagg tggaggacat ctgggtcctc acccgttttg
     2041 acgactgtct ggccgccttc agcgactggc agacctggtc atcggagcgg cgcggcaacc
     2101 tcctcaacga cctgcccgag cggatcggcc gcacgctggg cacgaccgac ccgccgcgcc
     2161 acacgggggc ccgccggctg gtgaataccg ccttcacgcc gcgcatcatc gccgggctgg
     2221 aaccgctgat ccgtgagctg tcggccagcc tacacgatgc ggcctgtggc aaggggacgt
     2281 tcgagtacgt ccaggacgtg tcggcgccgc tcaacgcccg catcctgggc gccatgttcg
     2341 gcgtccccga ggaggagttc cttcgggtgc ggggctggct cgacgatttc ttcgtgcggg
     2401 acaagcccga gccggggcgc gagccgcgcc aggtgacggc catgcgccag ttgcgcgcct
     2461 acgtcgacac gctggtggcg gaccgcctct gccggccggg tgacgacctg atgtccgcca
     2521 tgctccgcgt gcgcgagggg gacgtttccc tcagccacga gcaagtcgcg gtcaccacca
     2581 tgaccttcct cacggccggg ttcgagagca ccaacaatct cttcacgaac atggtggctg
     2641 cactcgccgg gcatccggat gtgctggcac gcctccggcg agagcctgcg gccgtgccgg
     2701 cattcacgga agaggcaatg cgctgggatg ccgccgccca gggcttcgtg cgcaccccga
     2761 cgcgggacgt ggagctgcac ggcagggtta tcccagccgg ggcccaggtg ctcgtccaca
     2821 tcggcgcggc caatcgtgat cctcgcgcct tccccgatcc cgatcgcttc gatctagacc
     2881 gggtcagctc gcggcatctc ggtctcggcc aggggaccca tttctgcatc ggcgggccgc
     2941 tggcacgggt gctggcccgc gtggccgtcg aagacctcgt ggcccgggcg gcgcgcatcc
     3001 agatcgacct gcgaacggct gtgcgggtca gcacacccaa cttc
//
LOCUS       DBNGZC010000755         1212 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_362316, whole genome shotgun sequence.
ACCESSION   DBNGZC010000755 DBNGZC010000000
VERSION     DBNGZC010000755.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1212)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_362316"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1021
                     /locus_tag="AC16G0_12950"
     CDS             167..1021
                     /locus_tag="AC16G0_12950"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR5100558.1"
                     /translation="MRIGAHAQIWVAPFTGNTLDLVGRVRDMGFDAIDINVGELPPPF
                     QAAELRRRLTESHLQGSLGAFLSAQRDITSEDAGVRRTGVEFLRGVARLCGEVGAKTF
                     IGPMHSEIRRQRPDPAPVREERWKRCVEGLAAVAREAEQVGIHVAVEVLNRYESDFLT
                     TTADGVRLCHAVGNPRLGLLLDTFHMNIEEQRIGDAFRSAAGHVYHVHTCENDRGAPG
                     SGHLGWVEVRNALRDIGYDGVCTIESYNPDLPMLAQRTSFWRPVASSPDELAREGLDF
                     LRRLFDSQ"
BASE COUNT          209 a          365 c          422 g          216 t
ORIGIN      
        1 cttcatgagg gcagggtgat ggccaaggga agggcggagg acctcgagaa gtcccgcgag
       61 atccgggaag cgtacctcgg gatgtagcgg attcatctcc cacggggaac gctcgccccg
      121 tgcgccggac gggaagacgg cagcagttga ggagggacgg gcacccatgc gaatcggggc
      181 gcacgcgcag atctgggtcg cgccattcac agggaataca ctcgacctgg ttggccgggt
      241 ccgggatatg ggcttcgatg cgatcgacat caacgtgggg gagcttcccc ctccctttca
      301 ggcggccgaa ttgcgacgtc gcttgaccga atcccatctc caggggtcgc tgggggcgtt
      361 cctcagcgcc cagcgggata tcaccagcga ggacgcgggg gtgcgccgga ccggcgtcga
      421 gttcctgcgg ggggtggcgc gactctgcgg ggaggtgggc gccaagacct tcatcggtcc
      481 catgcactct gagattcgac ggcagcgtcc ggaccctgcg cccgtgcgcg aggagcgatg
      541 gaagcgctgc gtggagggat tggcggcggt ggctcgggag gcggagcagg tcgggatcca
      601 tgtggcggtg gaggtcctga atcggtacga aagtgatttc ctcacgacca cggccgacgg
      661 ggtccgcctc tgccacgccg tggggaatcc ccggctgggt ctcctgcttg acacctttca
      721 tatgaatatc gaggaacagc ggatcgggga cgccttccgg tcggccgccg gacacgtcta
      781 tcacgtccac acctgtgaaa atgatcgggg cgcccctggg agcgggcatc tgggctgggt
      841 cgaggttcga aacgccttgc gggacatcgg ctacgacggc gtgtgcacca tcgaatcgta
      901 caacccggac ctgcccatgc tcgcccagcg aacttccttc tggcggcctg tcgcttccag
      961 cccggatgag ctggcgcgcg agggtctgga cttcctccgt cggcttttcg attcccagta
     1021 atgatcgagt ccgtctggac ggcaagccgg ctggctgcct gcgtggggaa gcccacggcc
     1081 tgaaaaggct ttgatgcttc cccaaccatg cgttacgcgg cagcgcactg ccgacaagca
     1141 gtggtgatca ccaatgaaga cttggcgctc cagtgtcctg gaatcaatgc cttggccttc
     1201 tctcggtacc ag
//
LOCUS       DBNGZC010000756         3882 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_652265, whole genome shotgun sequence.
ACCESSION   DBNGZC010000756 DBNGZC010000000
VERSION     DBNGZC010000756.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3882)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3882)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3882
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_652265"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="AC16G0_12955"
     CDS             complement(<1..473)
                     /locus_tag="AC16G0_12955"
                     /inference="COORDINATES: protein motif:HMM:NF028500.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR5100559.1"
                     /translation="MSDPVGVTLETPVEHLKGIGPSRAECLARLGIRTVGEALLLVPR
                     RYEDRRALLSIGRLRLGAFQSVAGAVKAVGMGRTRRGIPYAEVMLDDGTGTLLARWYR
                     QPYLARTFRRGQRVILAGRLNPYPPREMINPDHEIQEAADSHYHTGRIVPIYPLTA"
     gene            563..757
                     /gene="rpmB"
                     /locus_tag="AC16G0_12960"
     CDS             563..757
                     /gene="rpmB"
                     /locus_tag="AC16G0_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR5100560.1"
                     /translation="MPATRCEVCGKGQQYGAQVSHAHNVSTKRRYPNVQRVHAIVNGT
                     RRYIHVCTRCLRSGKVQKAA"
     gene            complement(827..2992)
                     /locus_tag="AC16G0_12965"
     CDS             complement(827..2992)
                     /locus_tag="AC16G0_12965"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR5100561.1"
                     /translation="MRIEAVLDRIHEYNTQADVGPVMRAYVFAAKAHKGQERISGEPY
                     LTHPLEVASILTEIRMDTATITAGLLHDAVEDTHATLKEIKEHFGDEISAIVDGVTKL
                     SRIPFSTREEAQAENIRKMILAMSKDIRVILVKLADRLHNMRTLEPLPDAKRRLIAQE
                     TLDIYAPLAHRLGIYWIKAELEDLALRWLDPVAYEDLAARIARKRQEREGDINEVIQL
                     LERKLHEVDIKAQITGRPKHFYSIYKKMRDQKKDFDEIYDLTAIRVITDSLKDCYGAL
                     GVIHTLWKPISQRFKDFIAVPKSNGYQSLHTTVIGPKGEPFEIQIRTREMHRVAEEGI
                     AAHWKYKEGRTNFDPTDESFVWLRQIMEWQRELKDSKEFLDTLRVDLFPDEVYVFTPR
                     GDVKPFPQGATPVDFAFAVHTDVGLRCVGAKVNGRLVPLRTELQNGDIVEVLTSPNHT
                     PSRDWLKIVKTPRARSKLRQWIKSEERARSVTLGRDMLEKELRRLGKSASQLLRPEAL
                     ALALERYGFATEDEFFAAVGYGKVSSRQAVGRLLPPEEFQALTEAAEGREKKAERKPA
                     RPRPAEDGVSIRGVDDILVRFSKCCNPVPGDEIIGFITRGRGVSVHTADCPNAVSLMA
                     DPERQIAVHWDGQRKGSHQVKIRVEIGKDRPGILAEISTAISATNTNIAQAEIRVTEE
                     RKGLNTFTLEVSDLRQLQAAMQAIQKVDGVVGVERVKSL"
     gene            complement(3198..>3882)
                     /locus_tag="AC16G0_12970"
     CDS             complement(3198..>3882)
                     /locus_tag="AC16G0_12970"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR5100562.1"
                     /translation="PQSVSGHATGGEVIVKSRVLSVNPKAPQVEVIRQACEVLRCGCL
                     VAFPTDTLYALGASALNAAAIERVLTVKGRHHGKPLSVLVGSVETAAGLAVGLSDEVR
                     SLMHSFWPGALTVVVKASPSLPSLLTAATGNVGLRMPGGAVAQSLLSTFAGPIIGTSA
                     NKSGGADPADARTVQKAIGGQIDLILDGGRVALGVPSTVIDCTTTPVRILREGAISRA
                     KIQEKIAIA"
BASE COUNT          616 a         1298 c         1253 g          715 t
ORIGIN      
        1 gcggtcaacg gatagatggg aacgatccgg cccgtgtggt agtgcgaatc cgctgcctcc
       61 tggatctcgt ggtcggggtt gatcatctcg cgggggggat aggggttcag ccggcctgcc
      121 aggatgaccc gctgcccccg gcggaaggtc cgggccagat agggctgccg gtaccagcgc
      181 gccaagagcg tcccagtccc gtcgtccagc atcacctcgg cataggggat gccccggcgc
      241 gtgcggccca tgccgaccgc cttgactgcg cccgcgacac tctggaaggc ccccagacgc
      301 aagcgtccga tggacagaag cgcccgccga tcctcgtagc gccgcggaac caggagaagc
      361 gcctcgccca ccgtccgaat ccccaggcgc gccaggcact cggcccgcga ggggccgata
      421 cccttgaggt gctccacggg ggtctctagc gtcacgccca cggggtcgga catccgagaa
      481 aaggcttgcc ttccaggggt ggattgagta tatttcgtcg cctttaatta gcggaacctg
      541 acgacgacag gagaatgtac cgatgcctgc cacccgatgt gaggtttgcg gcaagggtca
      601 gcaatacggc gcccaggtga gtcacgccca caacgtatcc accaagcgcc gctatcccaa
      661 cgtgcagcgc gtccatgcca tcgtcaacgg aacgcgccgc tatatccatg tctgcacccg
      721 ctgtctccgc tccggcaagg tccagaaagc tgcctgatcc ccctgtctcg gacttcgcgg
      781 gcgagcctgc aggtcttggt gaagccgagc gcctggcaca caaggctcac agcgacttga
      841 cccgttccac acccaccacg ccatccacct tttgaatcgc ctgcatggcc gcttgcagtt
      901 gccgcaggtc gctcacctct agggtgaagg tgttcaggcc cttgcgctcc tccgtcaccc
      961 ggatctccgc ctgggcgatg ttggtgttgg tcgcggagat cgccgtgctg atctcggcca
     1021 ggatgccggg ccggtccttc ccgatctcca cccggatctt cacctgatgg gaccccttgc
     1081 gctggccgtc ccagtgcacg gcgatctgcc gctcgggatc cgccatcagg ctcaccgcgt
     1141 tggggcagtc cgccgtatga accgaaaccc cccggccgcg ggtgatgaag ccgatgatct
     1201 cgtcgccggg gaccgggttg cagcacttgg agaaccggac caggatatca tccacccccc
     1261 ggatgctcac cccatcctcg gccggccggg ggcgcgcggg cttgcgctcg gccttcttct
     1321 cccggccttc cgccgcctcg gtcagcgcct ggaactcttc gggcggaagg agccgtccca
     1381 cggcttgccg cgaggagacc ttgccgtagc ccacggcggc aaagaactcg tcctccgtgg
     1441 cgaagccgta gcgttcgagc gccagcgcca gcgcctcggg ccggaggagt tggctcgcgc
     1501 tcttgcccag gcggcgcaac tccttctcta gcatgtcgcg gcccaaggta acgctccggg
     1561 cccgctcctc gctcttgatc cactggcgga gcttgctccg ggcccggggg gtcttgacga
     1621 tcttcagcca gtccctgctg ggcgtgtggt tcggcgaggt cagaacctcg acgatgtcgc
     1681 cgttctgcag ctccgttcgg aggggcacca ggcggccgtt gaccttggcc cccacgcagc
     1741 gcagacccac gtcggtgtgg acggcgaagg caaaatccac gggggtcgcg ccctggggga
     1801 agggcttgac gtcgccccgc ggggtgaaga cgtacacctc atcgggaaag agatccaccc
     1861 gcagggtgtc caggaattcc ttggagtctt tcagctcgcg ctgccactcc atgatctgcc
     1921 gcagccagac gaagctctca tcggtcggat cgaaattggt ccgcccctcc ttgtacttcc
     1981 agtgggcggc gatcccctct tccgccaccc gatgcatctc tcgggtgcgg atctggatct
     2041 cgaacggctc ccccttgggg ccgatgaccg tcgtgtgcag ggactggtag ccgttgctct
     2101 tgggcaccgc gatgaaatcc ttgaagcgct gggagatcgg cttccacagg gtatggatga
     2161 ctcccagggc gccgtagcag tccttgaggg aatcggtgat cacgcggatg gcggtgaggt
     2221 cgtagatctc gtcgaagtcc ttcttctgat cccgcatctt cttgtagatg ctgtagaagt
     2281 gcttcggccg gccggtgatc tgggccttga tatccacctc gtggagcttc cgctccagca
     2341 gctggatgac ctcgttgatg tccccctcgc gctcctggcg ctttctcgcg atgcgcgccg
     2401 ccagatcctc gtaagccacc ggatccagcc agcgcagcgc caggtcctcc agttcagcct
     2461 tgatccagta gatccccagc cggtgggcca gcggcgcgta gatgtccagg gtctcctggg
     2521 cgatcagccg gcgtttggcg tcgggcagtg gctccagcgt gcgcatgttg tgcaagcggt
     2581 ccgccagctt caccagaatc acccggatgt ccttggacat ggccaggatc atcttgcgga
     2641 tattctccgc ctgcgcctcc tcgcgggtgc tgaaggggat ccgcgacagc ttggtgaccc
     2701 cgtcgacgat ggccgagatc tcgtcgccga aatgctcctt gatctccttc agggtggcgt
     2761 gggtatcctc caccgcgtcg tgcaacagac cggcggtgat ggtggcggta tccatgcgga
     2821 tttccgtgag gatggacgcg acttcaaggg gatgggtgag gtagggctcg ccggagatcc
     2881 gttcctgacc tttgtgcgcc ttggcggcga acacgtacgc ccgcatgaca ggtccgacgt
     2941 ccgcctgtgt gttgtactca tggattctgt caaggaccgc ttcgatgcgc actcgacgac
     3001 tccgcccgcc ctgccgcggc gactcagtat aacacctgac ttttcggccg tacaagggct
     3061 atctggcctg acgcactcct gccccaccgc tcgcccccgc ttgtgggcgg gcttcggcat
     3121 ggggacggtc ttcggcgtgg tcaggtggca cctgccggag cggcgggagg tccgggcatt
     3181 cgtcgccagg cgggacgtca ggcgatggca atcttctcct ggatcttcgc ccgagagatg
     3241 gcgccttcgc gaaggatgcg aacaggggtt gtggtgcagt cgatcaccgt cgagggaaca
     3301 cccagcgcca cccgcccgcc gtcgagaatc agatcgatct gtccgccaat ggccttctgg
     3361 acggtccggg catcggcggg atcagccccc ccggacttgt tcgcgctggt gccgatgatc
     3421 ggtccggcga aggtggacag caaggactgg gccacggcgc cgcccggcat ccggagtccc
     3481 acattgcccg tagccgcggt gagcagggag gggagcgagg gagacgcctt caccacgacg
     3541 gtgagcgccc cgggccagaa gctgtgcatg aggctgcgca cctcatcgga caggccgacg
     3601 gccaatccgg ccgccgtctc caccgagccg acgagcacgc tcaggggctt cccgtggtgg
     3661 cggcctttca cggtgagaac ccgctcgatg gccgcggcat tcagggcgct cgccccgagg
     3721 gcgtagagcg tgtccgtggg gaaggccacc agacagccac agcgcaggac ctcgcaggcc
     3781 tgacggatca cttcgacttg gggggctttc ggattcacgg aaagcacgcg gctcttcacg
     3841 atgacctctc ctcctgtggc atgtccgctc acggattgcg gt
//
LOCUS       DBNGZC010000757         9085 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1436138, whole genome shotgun sequence.
ACCESSION   DBNGZC010000757 DBNGZC010000000
VERSION     DBNGZC010000757.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 9085)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9085)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9085
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1436138"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1155
                     /locus_tag="AC16G0_12975"
     CDS             223..1155
                     /locus_tag="AC16G0_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR5100563.1"
                     /translation="MQALFGQGDEHARLVEEGLHVRLVVRDGMLLISGAEGSVGIAEQ
                     VLGELVTLVRQGRSVGSQEVRLALRHFSQNGPESFRELQAEAIEVSARKRPVRPKSLG
                     QKRYIDAIRAHDIVFGIGPAGTGKTYLAMAMAVTALLHKEVHRIILARPAVEAGEKLG
                     YLPGTLTEKINPYLRPLYDALYDMLETERAMRLIEQGTIEIAPLAFMRGRTLNDSFII
                     LDEAQNTTSEQMKMFLTRLGFGSKTVITGDITQVDLPAGRVSGLIEVRAILKDIPGIR
                     FIHFTEEDVVRHELVQQIVRAYEAYQGPPTDPSA"
     gene            1157..2815
                     /locus_tag="AC16G0_12980"
     CDS             1157..2815
                     /locus_tag="AC16G0_12980"
                     /EC_number="3.1.4.59"
                     /inference="COORDINATES: protein motif:HMM:NF019318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0106409 - cyclic-di-AMP phosphodiesterase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD family phosphohydrolase"
                     /protein_id="HIR5100564.1"
                     /translation="MDLEFFNKSVDHELADRPPTPLRTGWHPFAPIRRTVVQLEQQRW
                     FLPTISAFFLLATVEILGSNLILPLGSLSILGVFLLVLFLLGALYAYVRGLQPKEVRR
                     PRSLALFGAVLLTVLLLNRYGLVVITALHQSLPEVPVSALRAALPVSLGGVLLTVLFN
                     ARLAFAGALALTILTSLMLQAPLDYFVFAFVGNMVSIFSLVGRQKRTAFFRAGSLLAV
                     ANAYCLLALALIGGYLSTLRAEVVGGLMNGLIVAILATGLLPLLEQSFGRTTNFTLLE
                     LSSLDEPMLKHLILTAPGTYNHSIMMGTLAEAAAEAVGANPLLCRVGAYYHDIGKTRH
                     PSYFIENQPDAVNRHDKLAPSLSRAIVMSHVKEGIEMARAYGLPEVLVDMIPQHHGTR
                     LVSYFYQRAKEACDPGLQAVKEEDYRYPGPKPQTREAAIIMLADAVEATTRSLTDPTP
                     ARIRGTVQKIITSIFVDGQLDECDLTLLDLHLIANSFVRILTGVFHHRVEYPGDEAQD
                     ATRKRSENGDQPAKPTAEGPRRDEVAKKPGGKGPVRPDAGEGGV"
     gene            2703..3182
                     /gene="ybeY"
                     /locus_tag="AC16G0_12985"
     CDS             2703..3182
                     /gene="ybeY"
                     /locus_tag="AC16G0_12985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR5100565.1"
                     /translation="MAISLRNRQQKVHVETRWLRNLGGKALSALMREKAECSVAVVDD
                     ATMAILNHTYRGVSGPTDVLAFSMTEGRFGGLSPDLLGDVVISAETAERQARDAGRDL
                     REELAHLLIHGLLHLVGYDHGTDQERQRMWRKERAILTACGIDAAAGGRRVGTATSK"
     gene            3221..4138
                     /locus_tag="AC16G0_12990"
     CDS             3221..4138
                     /locus_tag="AC16G0_12990"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR5100566.1"
                     /translation="MSRSSSRSPRASGFRLPWGAWFALLVSLPGLASAGEAMPVVSLA
                     PDHVLQGGIATLRIEWPGPIRALRIQVGDREILTAVPVDRAPTTLLVGIDLETPPGPL
                     EVRIDAEDRTGQALKARGTLQIQAAGYPVQRLTVPKAFTDLDAATLERINREKVVLDH
                     LWGVETPTRFWHERFRLPLDGAASGSGFGVRRIINGEPRAPHTGLDFTAAAGTPVLAV
                     NAGMVAAVEDQFFAGKAVILDHGLGLYSMYFHLQESLVKPGQRVERGERIAFVGNTGR
                     TTGAHLHWGARLHGARIDPRELLKPISLE"
     gene            4273..5217
                     /gene="era"
                     /locus_tag="AC16G0_12995"
     CDS             4273..5217
                     /gene="era"
                     /locus_tag="AC16G0_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020877330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR5100567.1"
                     /translation="MELTKAEDKDAAGAEEAFRAGYVAIIGPPNAGKSTLVNRLVQQK
                     LSIVSSRPQTTWHRILGIKTLPRAQLLFLDTPGLHKSDAAFNQALVRAALAAMADADV
                     ILWVSDASRSEDPDDHLILEQLREKGLKAAAILALNKVDLVAKPLLLPLIARWQERYP
                     FVEIVPISATKGDNIDRLESLLIRYVPEGHPFYPPEELSDRPERFFIAEIIREKAFTL
                     LRQELPYAVAVEVEAVRAREGRDLTDVEATIYVEKESQKAIVIGTGGKMLKRIGETAR
                     PEIEAMLETKVFLRLWVKVLEGWRKDPEALRRFGYLEG"
     gene            5310..6059
                     /gene="recO"
                     /locus_tag="AC16G0_13000"
     CDS             5310..6059
                     /gene="recO"
                     /locus_tag="AC16G0_13000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR5100568.1"
                     /translation="MPLHSTEAIVVGGHDLAEADRIIVFYTQTLGKVRAVADGARRIR
                     TRFGGSLQLFTQGRLVYFERPNRTLHKVNEFAVVRSHHPLREDLDRIAFGSAAVEAVS
                     SGVEEEEAHPDLYRLLADGLDLLEEAARPALVMQGFLLHLLRLLGYRPELAECVHCHR
                     VLAGGTEVRISPGQGGVLCPNCWQGYVDALSVSSEALGFLRGAGGSSLRVMDRITLPP
                     LVLQEVSEIMQAFLQHVLGRRLRSADFLSRL"
     gene            6179..7078
                     /gene="glyQ"
                     /locus_tag="AC16G0_13005"
     CDS             6179..7078
                     /gene="glyQ"
                     /locus_tag="AC16G0_13005"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HIR5100569.1"
                     /translation="MTFQELILALESFWAKYGCVIQQPYDVEVGAGTMNPATFLRVLG
                     PEPWNVAYVEPSRRPADGRYGENPNRLQHYYQYQVILKPSPLDIQEIYLQSLKSFGVD
                     PLKHDISFVEDDWESPTLGAWGLGWEVWLDGLEITQFTYFQQAGGIELRPVAGELTYG
                     IERIAMFLQGVDNVYDLQWNQGVTYGDVHHKGEVEWSVHNFEAADVPLQLRLFEEYER
                     ESARLIENGLVLPAYDYCLKCSHTFNILDSRGAIGVTERTHFIGRVRALARRTAEAYL
                     KQREEMGFPLLKTVPPGARGVSQ"
     gene            7201..>9085
                     /gene="glyS"
                     /locus_tag="AC16G0_13010"
     CDS             7201..>9085
                     /gene="glyS"
                     /locus_tag="AC16G0_13010"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HIR5100570.1"
                     /translation="MGNDLLFEIGTEDIPSGYIPPALEELKAVAVRLLQEQRLNFVAA
                     RTLATPRRLTLFVDGLAERQADARREVVGPPKAVAFDADGNPTKAAQGFARAQGVPVE
                     SLEVRALDRGEYLVAVQEEKGARTPEILATLLPKLITVLSFPKFMRWGEGTFRFVRPI
                     RWLLAVYAGKVVPISVDGLQSDAKTYGHRFLSPRGVRVRNFEEYLSKLEEKYVIASTD
                     RRRALVRTLVIEAAAAVKGQPILENDLLETVTNLVEFPTAVCGSFKPEYLELPREVII
                     TPMQKHQRYFPVVDASGKLLPHFVTVSNMRAKDMDLIRQGNERVLRARLNDAEFFYKE
                     DRKHPLAERIPQLQHIIFQEKLGTVHAKVERLRRLVGLIAERADPACRAVALRAAELC
                     KADLVTSMVKEFPTLQGVMGGDYARLSGEPPEVCQAIVEHYLPRFAGDKLPESNAGAV
                     VGLADRLDSIVGCFGVGLIPTGSEDPYALRRAALGLVQTILQRRLRAPLAELVAAAQA
                     GFGPLLTRDAEKTRREVLDFLRSRMQAVLVERGLPADVVEAALSAGSDDLVDATRRAE
                     ALVAMRREMDFGELSAAFRRVVGIIPKGFDRAVEVGRLVEGAERALYAQTAELKTEVD
                     QLVA"
BASE COUNT         1544 a         2947 c         3036 g         1558 t
ORIGIN      
        1 gggccgaggt cggtctggcc cgtccgacgg ccgtgggggg aggggctgat tcaccgccgt
       61 ttccccgcct cccttgaccc tttcccattg acagcccctg gggccgggtc tatccttccg
      121 ttacgcaaac ggctcgacac aagcggaggt tggcgcagct cctgtatggg tgaaaagggg
      181 agcgaagctt cgaagcgaat atccctggcc gcaggcccgg agatgcaggc gctgttcggc
      241 cagggagatg aacatgcccg gctggtcgag gaggggctgc acgtccgcct ggtggtgcgc
      301 gacggcatgc tcctgatctc cggtgccgag ggcagcgtcg gcatcgcgga gcaggtcctc
      361 ggggaattgg tgacgctggt ccgtcagggg cgttccgtag gcagccagga agtccgactg
      421 gctctgcgcc acttctccca gaacggcccg gaaagcttcc gcgagctgca ggctgaggcg
      481 atcgaggtct cggccaggaa acgcccggtc cggcccaaga gcctcggaca gaagcgctac
      541 atcgacgcca tccgcgcaca cgacatcgtc ttcggcatcg gcccggccgg cacgggcaag
      601 acctatctcg ccatggccat ggccgtgacg gccttgctgc acaaggaagt gcaccgcatc
      661 atcctggcgc ggcctgcggt ggaggcgggc gaaaagctcg gctacctccc cggcaccctc
      721 acggagaaga tcaaccccta cctgcgaccg ctctacgacg cgctgtatga catgctcgag
      781 accgagcggg cgatgcgcct gatcgagcag gggaccatcg agatcgctcc cctggcattc
      841 atgcgtgggc ggacgctgaa tgattccttc atcatcctgg acgaggccca gaacaccacc
      901 tcggagcaga tgaaaatgtt cctcacgcgt ctgggctttg gctccaagac ggtgatcacc
      961 ggggacatca cccaagttga tctccccgct ggtcgggtgt ccggcctgat cgaggttcgc
     1021 gccatcctga aagacatccc cggcatccgg ttcatccact tcaccgaaga ggacgtggtt
     1081 cgccacgagt tggtccagca gatcgtccgg gcgtacgaag cctaccaggg tccccccaca
     1141 gacccgagtg cttagcatgg atctggaatt cttcaataag tcggtcgacc acgaactggc
     1201 ggaccggccg cccaccccgc tccggacggg ctggcacccc ttcgccccca tccgccggac
     1261 cgtggttcaa ctggagcagc agcgttggtt cctgccgacc atctcggcct tcttcctgct
     1321 ggccacggtc gagatcctgg gctccaacct gatactcccg ctggggtcct tgtccatcct
     1381 gggggtcttc ctgctggtcc tctttctcct cggggcgctc tacgcctacg tgcgcggcct
     1441 ccaacccaag gaggtccggc gtccgcgcag cctcgccctg ttcggggcgg tgctcctcac
     1501 ggtgctgctc ctcaaccggt acggcctggt ggtgatcacc gccctgcacc agagcctccc
     1561 ggaggtcccg gtctccgcgc tcagggccgc gctgccggtg tccctggggg gcgtcctgct
     1621 cacggtgctg ttcaacgccc gactcgcctt cgcgggagcg ctcgccctca cgatcctcac
     1681 gagtctcatg ctccaggccc ccctggatta tttcgtcttt gccttcgtgg ggaacatggt
     1741 gagcatcttc agtctggtgg ggcgacagaa gcggacggcc ttcttccgcg ccggaagcct
     1801 gctggccgtg gccaacgcct actgcctgct ggccctggcc ctgatcgggg gttatctcag
     1861 cacgcttcgc gccgaggtgg tgggtggcct gatgaacgga ctcatcgtcg ccattctggc
     1921 cacgggtctc ttgccacttc tagagcagtc cttcgggcgg acgacgaact tcacgctcct
     1981 ggaactgtcc agcctggacg aacccatgtt gaagcacctg attctcaccg ctccgggcac
     2041 atataaccac agcatcatga tgggcacgct ggcagaggcc gcggcggagg cggtgggggc
     2101 gaatcccctc ctctgccggg tcggcgcgta ctaccacgac atcggcaaga ccagacaccc
     2161 ctcatacttc atcgagaatc agccggacgc cgtgaaccgc cacgacaagc tggcgcccag
     2221 cctgagccgg gccatcgtga tgtctcacgt gaaggaaggg atcgagatgg ccagggccta
     2281 cgggctaccc gaggttctgg tggacatgat cccccagcat cacgggacgc gactggtgag
     2341 ctacttctac caacgcgcca aggaagcgtg cgatccgggc ctccaggcgg tgaaggagga
     2401 ggattaccgc taccccggac cgaagcccca gacgcgcgag gcggcgatca tcatgctggc
     2461 ggacgcggtc gaggccacga cccgtagcct gacggatccg acgccggccc ggatacgagg
     2521 gacggtgcaa aagatcatca ccagcatctt cgtggatggc cagttggacg aatgcgatct
     2581 caccctgctg gatctccacc tcatcgccaa cagctttgtc cggattctga cgggggtctt
     2641 ccaccatcgg gtcgagtatc cgggcgacga ggcccaggac gccacccgga agcggagcga
     2701 gaatggcgat cagcctgcga aaccgacagc agaaggtcca cgtcgagacg aggtggctaa
     2761 gaaacctggg gggaaaggcc ctgtccgccc tgatgcggga gaaggcggag tgtagcgtgg
     2821 ccgtggtgga cgatgccacc atggcgattc tgaaccacac ctaccgaggg gtatccggcc
     2881 caaccgacgt cctggccttc tccatgaccg aggggcgctt cggcgggctt tcgccggatc
     2941 tcctgggcga tgtggtgatc tctgcggaaa cggcggagcg tcaggcgcgg gatgccggcc
     3001 gcgacctccg ggaggagctg gcccacctct tgattcacgg actcctgcac ctcgtcggct
     3061 acgatcacgg cacggaccag gaacgccagc ggatgtggcg gaaggagcgg gcgatcctca
     3121 cggcgtgcgg gatcgacgct gcggccggcg gtagacgtgt cggaacggcg acctcgaagt
     3181 gaaccccaca aatgcccggc tcgcggtctc cgcgagccag atgtcacgca gctcgagccg
     3241 atccccccgg gcgtctggtt tccgcctgcc atggggcgcc tggtttgctc tcctggtaag
     3301 cctgcccggc ctggcctctg ccggcgaggc gatgccggtc gtcagtctgg cgcctgatca
     3361 tgtcctgcag ggagggatcg caaccctcag aatcgagtgg cccggcccga ttcgcgcgct
     3421 ccgcatccag gttggagatc gggagatcct cacggctgtc cctgtggacc gggcgcccac
     3481 cacgctcttg gtcgggatcg atctggagac gccgccgggc cccctcgagg tccgcatcga
     3541 tgccgaggat cggactggtc aagcgctgaa agcccgagga acactccaga tccaggctgc
     3601 cggttaccct gtccagcgac tcacggtccc caaagcgttc accgatctgg atgcggcgac
     3661 cctggagaga atcaaccgcg agaaggttgt cctggaccat ctctggggtg tggagacacc
     3721 gacgcgattc tggcacgagc gattccggct ccccctggac ggggcggcct ccggctccgg
     3781 gttcggcgtg cggcggatca tcaacggcga gccgcgtgcg ccccacacgg gcctcgattt
     3841 cacggcggcc gccgggacgc cggtcctggc cgtgaatgcg ggaatggtgg cggcagtgga
     3901 ggaccagttc ttcgccggca aggcggttat tctggatcac ggtctgggtc tatacagcat
     3961 gtacttccac ctgcaggaaa gtctggtgaa gcccgggcag cgggtggagc ggggggagcg
     4021 cattgccttt gtggggaata ccggccggac cacaggcgcc catctccact ggggggcgcg
     4081 acttcacggg gcgcgcatcg atccgcgaga actcctgaag ccgatctcgt tggagtgatc
     4141 gggcaaggga tacacgaaac gttcgggtgt tcggccgtgc ctggaacagt cgaaccgtcg
     4201 gctccgtccc gagccgcgtg ggggagcggg gacggccgag ctgccctacg tctttggtac
     4261 agcgggaagc gcatggaact caccaaggcc gaagacaaag atgccgcggg agcggaggaa
     4321 gccttccgcg ccgggtacgt cgccatcatc ggaccgccga acgcgggcaa gtccacgctc
     4381 gtgaacagac tggtccagca gaagctctcg atcgtctcct cgcggcccca gaccacctgg
     4441 catcggatcc tgggaatcaa gactcttccc cgggcgcagc tcctcttcct tgacactccg
     4501 ggcttgcaca agtcggacgc ggcgttcaac caggccctgg tccgggcggc cctcgccgcc
     4561 atggcggacg ccgacgtgat cctgtgggtg agcgacgcct cccggtccga agacccggac
     4621 gatcacctga ttctggagca actccgcgag aaggggctca aggccgcagc catcctggcg
     4681 ctgaacaagg tggacctggt cgcgaagcca ctgctgctgc ctctcatcgc ccgctggcag
     4741 gagcgctacc cctttgtcga gatcgtaccc atctctgcca ccaaggggga caacatcgac
     4801 aggctcgagt cgctcctgat ccggtatgtc ccggaagggc atccgttcta tcctcccgaa
     4861 gagctgagcg atcgcccgga acggttcttc atcgccgaga tcatccggga gaaagccttc
     4921 accctcctcc gccaagagtt gccctatgcc gtggccgtcg aggtggaggc cgtgcgggcc
     4981 agggagggac gggatctcac cgacgtggag gcgacgatct acgtggagaa ggaatcgcag
     5041 aaagcgatcg tcatcggcac cggcgggaag atgctgaaac gcatcggcga gaccgcgcgc
     5101 cccgagatcg aggcgatgct ggagacgaag gtgttcctgc ggctctgggt gaaggtgctg
     5161 gagggctggc gcaaggaccc ggaggccctg cggcgcttcg ggtacctgga aggatgacga
     5221 cagtgcgaat ttcgaattgc gaatttcgaa cttgaaatcc cttccccgca ttccgaaatt
     5281 cgaaatccgc aattcgacat cgaggaccca tgcccctgca ttccaccgag gcgatcgtcg
     5341 tcggcggcca cgacctggcg gaggcggacc ggatcatcgt gttctacacc cagaccctgg
     5401 gaaaagtccg ggcggtcgcg gatggggcac ggcggatccg cacgaggttc ggcggcagcc
     5461 tgcaactctt cacccagggg cggctggtct atttcgagcg gccgaaccgg acgctccaca
     5521 aggtgaacga gttcgccgtc gttcgatcgc atcatcccct gcgggaggat ctggaccgga
     5581 tcgccttcgg gtccgccgcg gtcgaggctg tgtccagcgg cgtggaggag gaagaggctc
     5641 acccggatct ctaccgcctg ctggccgatg gactcgatct cctcgaagag gccgcccgtc
     5701 cggccctggt gatgcaaggc ttcctgctgc acttgctgcg attgttgggg taccgcccgg
     5761 agctggcaga gtgtgtccac tgtcaccggg tcctcgccgg cggcacggaa gtccggataa
     5821 gccctggcca gggcggtgtg ctatgtccga actgctggca ggggtacgtg gatgcgctct
     5881 cggtctcgtc ggaggccctg ggctttctcc gcggcgccgg cggctcgagc ctccgggtca
     5941 tggaccggat aacccttccg cccctggttt tacaggaagt tagcgagatc atgcaggcct
     6001 tcctccagca cgtgctgggc cggcgtctca ggtccgcgga cttcctgagc cggctgtaac
     6061 cctcgatcaa taaagccgtt gcgaaatcgc cgggggcctt ttcccttgac actctcgtgc
     6121 ggaatttgtt aggttgtcac ttcatatttc ccgctcggga tccacacgga ggcagacgat
     6181 gacgttccag gagctgatcc ttgccctgga gagtttctgg gccaagtacg ggtgcgtgat
     6241 ccagcagccc tacgatgtgg aagtcggcgc cggcaccatg aacccagcaa ccttcctgcg
     6301 ggttctcggc cccgagccgt ggaacgtggc ctacgtcgag ccgtctcggc gccccgcgga
     6361 cggccgctac ggggagaacc ccaaccggtt gcagcactac taccaatacc aggtcatctt
     6421 gaagccctcc cccctggaca tccaggagat ctatctgcaa agcctgaaga gcttcggcgt
     6481 ggacccgctg aagcacgaca tcagctttgt cgaagacgac tgggagtcgc ctaccctggg
     6541 ggcgtggggt ctcggctggg aggtctggct ggacgggctg gagatcaccc agttcaccta
     6601 tttccagcag gcggggggca tcgagcttcg gcccgtggcg ggggagctca cctacggcat
     6661 cgagcgcatc gccatgttcc tccagggcgt cgacaatgtg tatgacctgc agtggaacca
     6721 gggcgtcacc tacggcgatg tccaccacaa gggtgaggtg gagtggtcgg tgcacaactt
     6781 cgaggcggcg gatgtcccgt tgcagctccg gctcttcgag gagtacgagc gggagtcggc
     6841 tcgcctgatc gagaacggtc tggttctccc cgcctatgac tattgcctga agtgctccca
     6901 caccttcaac atcctggact cgcggggagc catcggcgtg acggagcgga cccacttcat
     6961 cgggcgggtg cgagccctgg cccggcggac ggccgaggcc tacctcaaac agcgtgagga
     7021 gatgggcttc ccgctgctga agaccgtccc gcccggcgct cgcggggtct cccagtagcg
     7081 acaggcggga cagggtggcc gcgccgcgca ggtgatgccc tgcatcgaga gccaacgacg
     7141 ctgaggtcgt ctcggatctc ctgacacggg gtgcgggcga aggcaaaagc acgggacgcc
     7201 atgggaaacg acttgctctt cgagatcggt accgaggaca ttccctccgg gtacatcccg
     7261 cccgccctgg aagagctgaa ggcggtcgcg gtacggctgc tccaggagca gcgcctcaac
     7321 ttcgtcgccg cccggacttt ggcgacgccg cggcggctga ccctgttcgt ggacggcttg
     7381 gccgagcgtc aagcggacgc gcggcgcgag gtggtcggcc cgcccaaggc agtcgccttc
     7441 gacgccgacg gaaatccgac caaggcagcc caggggttcg cccgcgccca gggggtcccg
     7501 gtcgagagtc tggaggtccg ggcgctggac cggggcgaat acctggtggc ggtccaggag
     7561 gagaagggag cgcggacgcc ggagatcctg gcgaccctgc tgcccaagct catcaccgtg
     7621 ctctccttcc ccaagttcat gcgctggggc gaggggacgt tccggttcgt ccggcctatt
     7681 cgctggctcc tggcagtcta tgccggcaag gtcgtcccca tctccgtgga cggcctccag
     7741 tcggacgcca agacgtacgg gcaccgcttc ctctcgccgc gcggggttcg ggtgcggaat
     7801 tttgaagagt acctgagcaa gctggaggag aagtacgtca tcgccagcac ggatcgtcgt
     7861 cgagccctgg tgcggacact agtgatcgag gcggcggccg cggtcaaggg ccagccgatc
     7921 ctggaaaacg atctcctgga gacggtcacg aacctggtgg agtttcccac agccgtctgc
     7981 ggctccttca agcccgagta cctggagctc ccccgggagg tcatcatcac cccgatgcag
     8041 aagcaccagc gctacttccc ggtggtggac gcctcgggga agctcttgcc gcacttcgtg
     8101 acggtgtcga acatgcgcgc caaggacatg gacctgatcc gccaggggaa cgagcgggtc
     8161 ctgcgcgccc gcctgaacga cgccgagttt ttctacaagg aagaccgcaa gcatccgctg
     8221 gcggagcgga tcccgcagct ccagcatatc atcttccagg agaagctggg gacggtccat
     8281 gccaaggtgg agcggctacg gaggctcgtc ggtctcatcg ccgagcgggc cgatcctgcc
     8341 tgccgggccg tcgccctgcg cgccgcggag ctgtgcaagg cggatcttgt caccagcatg
     8401 gtcaaggagt tccccacgct ccagggggtc atgggcgggg actacgctcg gctctccggc
     8461 gagcctccgg aggtctgcca ggcgatcgtc gagcattacc tcccccggtt cgcgggcgac
     8521 aagctgccag agtccaacgc cggcgccgtg gtgggcctgg cggaccgcct ggactccatc
     8581 gttggctgct tcggcgtcgg gctcatcccc accggctcgg aggaccccta cgccctccgc
     8641 cgggcggccc tggggttggt ccagacgatc ttgcagcggc gcctccgcgc gcccctggcg
     8701 gagctggtgg cagcagcgca ggccggcttc ggcccactcc tgacacgtga tgctgagaag
     8761 acgcggcgcg aggttttgga ctttctccgc agtcggatgc aggcggtgct ggtggagcgg
     8821 ggactgcccg cggatgtggt ggaggcggcc ctgtcggccg ggtccgacga cctggtggat
     8881 gccacgcggc gcgccgaggc gctggtcgcc atgcggcgtg agatggattt cggggaactg
     8941 tctgcggctt tccggcgcgt ggtgggcatc atccccaagg ggttcgaccg ggcggtggag
     9001 gtcgggagac tcgtcgaggg ggcggagcga gccctgtacg cgcagaccgc ggagctgaag
     9061 acggaggtcg atcagctcgt cgcca
//
LOCUS       DBNGZC010000758         1278 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_66922, whole genome shotgun sequence.
ACCESSION   DBNGZC010000758 DBNGZC010000000
VERSION     DBNGZC010000758.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_66922"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="AC16G0_13015"
     CDS             <1..188
                     /locus_tag="AC16G0_13015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5100571.1"
                     /translation="LVPPGDSSAIANAVSALLGDPHRASVLGEAGRRRAPRYSAETMV
                     SALAKLYHEVVEVARPS"
     gene            250..>1278
                     /locus_tag="AC16G0_13020"
     CDS             250..>1278
                     /locus_tag="AC16G0_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100572.1"
                     /translation="MALHLVLALGLIGMPRCPAGAAGSLHPVATAMHIHSNWSTGDST
                     LDALIARAQAAGVEVIFLTENHSLRFEYGVFPLRNLLRYRVEYPSLLKQGPGTFLEAV
                     RAANARQNDVLLVPGTEVSPYYYWTGNLFDGTLTMHDGEKKLLAFGLYRTEDYTALPS
                     VGNPDARVWGWASLGLLSPGLLAIPGLWLLRLRKKQVVHLQYFRMTVERRYTGYGILC
                     LAVGFVFLANNFPFRTAPVSAYDPNEGLRPFQAVIDAVLARGGLVGWSLPEARDYQVV
                     HVAGFQATVVTEPHPSALLGTDRFTAFGGVYEDTTAFTNPGGGWDVLLTEYLRGRRTA
                     PAWAIGEAA"
BASE COUNT          199 a          412 c          417 g          250 t
ORIGIN      
        1 tcctcgtgcc gccgggtgac tcgtctgcca tagccaatgc cgtcagcgcg ctcctgggtg
       61 acccgcatcg cgccagcgtt ctgggagagg ccgggcggcg gcgggccccc cgctacagtg
      121 ccgagacaat ggtgtccgcg ctggcaaagc tctatcacga ggtcgtggaa gttgcccgac
      181 cgtcgtagcc tgcccctcgg cctcgggagt gtccaagggc ggccggattt gctccgtcgt
      241 tgccgaagta tggccttgca tctcgtgctg gccctgggcc tgatcggaat gccgcgctgt
      301 ccagccgggg ccgctggatc cttgcatccg gtcgccacgg cgatgcacat ccactcgaat
      361 tggagtaccg gggattccac gctggatgcg ttgattgccc gggcacaggc ggcgggcgtg
      421 gaggtcatct ttctgaccga gaatcattcg ctgcgcttcg agtatggcgt attccctctc
      481 cgcaatcttt tgcgctatcg cgtcgaatac ccgtcgctct tgaaacaggg accgggcacc
      541 ttcctggaag ccgtgcgggc agccaacgcc cgccagaatg acgttcttct ggtccccggg
      601 acggaagtga gcccctatta ctactggacc gggaacctct tcgacggcac gctgaccatg
      661 cacgatgggg agaagaagct cttggccttc gggctctacc ggaccgaaga ctacactgca
      721 ctgccctcgg tcgggaaccc cgatgcgagg gtatggggat gggcgagcct ggggctgctg
      781 tcgcctgggt tgctcgccat cccgggcctg tggctgctgc ggctacgaaa aaagcaggtg
      841 gtgcacctgc agtattttcg aatgaccgta gaacgccggt acacgggcta cggcatcctg
      901 tgcctggcag ttggcttcgt gttcctggcc aacaacttcc ccttccgcac ggcgcccgtt
      961 tccgcctatg atccgaatga agggctgaga ccattccagg cggtcatcga tgccgtcctg
     1021 gcgcgcgggg gactggtcgg ctggtcgctc cccgaggcac gagattatca ggtggtccat
     1081 gtcgccgggt ttcaggcaac agtcgtgacg gagccccatc cctccgccct gttgggtacg
     1141 gaccgcttca cggccttcgg cggagtctac gaggacacca ccgcgttcac caatcccggg
     1201 ggtggatggg acgtcctgct gaccgagtat ctccgcggcc ggcgcactgc gcccgcgtgg
     1261 gcgatcggag aggccgcc
//
LOCUS       DBNGZC010000759         6090 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_554519, whole genome shotgun sequence.
ACCESSION   DBNGZC010000759 DBNGZC010000000
VERSION     DBNGZC010000759.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_554519"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="AC16G0_13025"
     CDS             <1..281
                     /locus_tag="AC16G0_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR5100573.1"
                     /translation="TDRAKEVLKQNGFAVSKTEVIGVVVPDRPGGLAEILGVLQSARI
                     NVEYMYAFVQRSGDNAIIIFRFDELDKAIQTLTGGGVRVLKGEELYAL"
     gene            complement(408..1565)
                     /locus_tag="AC16G0_13030"
     CDS             complement(408..1565)
                     /locus_tag="AC16G0_13030"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5100574.1"
                     /translation="MDITDLMLDPAVGKALDWAESHAGQIAEEAVRICEIPAPTFEEG
                     ERAKYVRGRFTALGLRDVTIDGAGNVRGRRPGTGNGPGLALAAHLDTVFPRGTDVKVK
                     RWGNRLAAPGIGDNSVAIAGLLGMVEALNAGGVQTRGDLYLASNTGEEGLGDLKGMKA
                     FMADVKDKVAAAIAVEGMKVNRLIHVAVGSRRYRVTYTARGGHSWGHFGSPSAIHLLG
                     RAIADISHLEVPKEPKTTYNVGVIHGGTTVNTIAAEADMLVDMRSVEPQALAELERRV
                     LAIIERAAQEGGGQAKLEMVGDRPAGSIPADHPVVQTCRAVHQALGLQTFSEPASTDH
                     NVPLGMGLPGVCLSITEGANEHRLDEYIETGPIPTGVKNILLAAIALTAGK"
     gene            complement(1601..2905)
                     /gene="dcuC"
                     /locus_tag="AC16G0_13035"
     CDS             complement(1601..2905)
                     /gene="dcuC"
                     /locus_tag="AC16G0_13035"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HIR5100575.1"
                     /translation="MLMNIIAAVVTVLTFAAIVKKVEVRLALLTGGLVMAVIAWNPQA
                     WSDAFARNMVVAGLLQTIPPVMGFAAVMEITGCNQHLVKLLTDPLIKIRPLLIPGAML
                     VTYFINMALASAAGCTAAVGVVLIPAMMAAGIHPGMAAASVFAGTWGSIWSPGNAHNA
                     MLSKIANVPVMDVITAHTSVSLPLAVFVAVMLFMEGKLFKQDRDWTPEKAGANLGQIA
                     KTEKVALERVNIWMAVTPAVPLLILLVSNVGWVTSTFTWIPKGVTVLVAMMIGTALGM
                     VVTWTGAPTIVTKFFDGMGHAYGFVFGIIIAAGAFIGGLEAAGVIKNFIEVLKSAQHA
                     VPFFATFGPFLVAVVGGSGDAATLAFNNAVTPHAAQFGIGTVALGNLANIGGALGRSL
                     SPVAAACIIAAGFAGVNPFEITKRNVVPMIIAATTAMLLLGR"
     gene            complement(2987..4402)
                     /locus_tag="AC16G0_13040"
     CDS             complement(2987..4402)
                     /locus_tag="AC16G0_13040"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5100576.1"
                     /translation="MGPRYDVLLRNGRVVDPATARDGVSDLAVADGKIAELAPEIDPT
                     QAGEVFDLRGFCVAPGVIDLHTHVSEWLGGKCGHRMMAQVGVTTALDMSGPVDSVMGM
                     ARDHGAGMNIACIEAVRPGGTVRDTNPGREELEAVLEAALQRGAIGLKLLGGHYPLTP
                     DATARAIEVANQRRAYVGFHVGTLEHGSNIDGFLQAVDLAAGNALHIAHVNSYCRGAV
                     RPYMQETEEAIVALEQHPHLCSESYLSPLNGTSAKCVGGAPESRVTQQCLRRGGFDAT
                     TAGMEEAILAGWAQINLEAGGQVILATGAAARDFWRQRGTDTAVSFAVNPAEPRVRLA
                     TARRPSGDFVVDCISTDGGGIPRNVIVDMGLALVALRALTLGEFVRKSSTNPARILGL
                     VNKGHFGLGADADITVLDVDRREAVLSLANGRVIMHRGYVCGHGSRMITTSAGAAAVR
                     RQGLEPIVVDLAKGAFYHRQA"
     gene            complement(4455..5537)
                     /gene="purM"
                     /locus_tag="AC16G0_13045"
     CDS             complement(4455..5537)
                     /gene="purM"
                     /locus_tag="AC16G0_13045"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR5100577.1"
                     /translation="MSEVNYTQAGVNTIKEEAALAGLLTWVGRTLAFGRYRSALDFGY
                     FATVLDLGNNLGLALSSDGVGTKLLVAEMLDKYDTVGIDCIAMNVNDLLCVGAEPICM
                     LDYIAVQEADPGLLTAIGKGLYEGARQANISIPGGEIAQVRELIRGVREGRGFDLAGT
                     CVGTVPMDRIIIGRTIEPGDAVIGLRSSGIHSNGLTLAREIFFRRAGYAVDRHVPELG
                     RSIGEELLEPTRIYVREVLALLHAGFDIRAMAHITSDGLLNLARVEAKVGYVLDSLPA
                     PQPIFSLLQRLGEVTDEEMFQVYNMGVGFCLMVAAAQADRILAALKDLGVEAFYLGRA
                     VADPARKVHLPNGLVGEGNAFRRDRL"
     gene            complement(5646..>6090)
                     /gene="metG"
                     /locus_tag="AC16G0_13050"
     CDS             complement(5646..>6090)
                     /gene="metG"
                     /locus_tag="AC16G0_13050"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HIR5100578.1"
                     /translation="FGVGGVRAMAKGEALFPRVEFSLEEAPKSATPASAQGTGEEISI
                     EEFGRLDLRVAQVLTAEAVPGSKKLVKLRIATGSEERTIVAGILIDYPPAELVGKQIV
                     IVANLKPTKLMGVESRGMLLAASGADGRLVLVTPERQAVPGSKVK"
BASE COUNT          995 a         2097 c         1936 g         1062 t
ORIGIN      
        1 agaccgaccg ggcgaaggag gtgctgaagc agaacggctt cgcggtgtcc aagaccgagg
       61 tgatcggggt ggtggttccg gaccggccgg gcggcctcgc agagatcctg ggggtgctcc
      121 agtcggccag gatcaacgtc gaatacatgt acgcctttgt ccagcgctcg ggcgacaacg
      181 ccatcatcat cttccgcttc gacgaactgg acaaggcgat ccagacgctg acgggtgggg
      241 gcgtgcgcgt actgaagggg gaggagctat acgcgttgtg aactcacgca gaggcgcaga
      301 ggcggaaaga ttgagggggt gaaagggaga gggggagcac gggagaaaga tctcccacgc
      361 cccctatctc ccgtgcacct tcacgtttct gttatctgaa cccgctctta cttcccggct
      421 gtcagggcga tggccgccag caggatgttc ttcactccgg tcggaatcgg gccggtctcg
      481 atgtactcgt ccagccgatg ctcgttggcc ccctcggtga tgctgaggca tacgccgggc
      541 aggcccatcc ccaggggtac gttgtggtcg gtgctggcgg gctcgctgaa ggtctggagc
      601 ccaagggcct ggtggaccgc ccggcacgtc tgcaccacgg ggtgatccgc cgggatggag
      661 ccggcaggtc ggtcgcccac catctcgagc ttcgcctgac caccaccttc ctgggccgcc
      721 cgctcgatga tggcgaggac tcgccgttcc agctccgcca gggcctgcgg ctccaccgag
      781 cgcatgtcca ccagcatgtc ggcttcggcg gcgatggtgt tgacggtggt cccgccgtgg
      841 atcacaccga cgttgtacgt ggtcttgggc tccttcggga cctccaggtg cgagatgtcc
      901 gcgatggcac gcccgaggag gtggatggcg ctgggcgagc cgaaatggcc ccagctatgg
      961 ccgcccctcg cggtgtaggt gactctgtat cgtcggcttc ccaccgccac gtgaatgagc
     1021 cggttgacct tcatcccttc caccgcgatg gcggctgcca ccttgtcttt cacatcggcc
     1081 ataaaggcct tcatcccctt cagatcccca agaccctcct ccccggtgtt ggaggccagg
     1141 tacaggtcgc cccgggtctg gacgccgcca gcgttgagcg cctcgaccat ccccaggaga
     1201 cccgcgattg ccacgctatt gtccccgata cctggggcgg ccaggcgatt tccccagcgc
     1261 ttcaccttca cgtcggtccc tctcgggaac accgtatcca ggtgggcggc cagcgccagg
     1321 ccggggccgt tgcctgtccc cgggcgtctc ccccgaacgt tacccgcccc atcgatcgtc
     1381 acgtcccgca ggcccagggc cgtaaatcgg ccgcgaacgt acttggcgcg ctccccctcc
     1441 tcgaaggtcg gtgccgggat ctcgcagatc cggacggcct cttcggcaat ttggccggcg
     1501 tgactttcag cccagtccag ggccttgccc acagccgggt ccagcatcag gtcagtgatg
     1561 tccatccgct tcctcctcgt tctggtcctc ggactgtctc ctaccgtccc aagagcaaca
     1621 tcgccgtggt ggcggcgatg atcatgggga ccacattccg cttggtgatt tcgaaggggt
     1681 tcacgccggc gaatcccgcg gcgatgatgc aggcggccgc caccggcgac aggctgcgtc
     1741 ccagcgcgcc cccgatgtta gcaaggttcc ccagggcgac ggtgccgatc ccgaactggg
     1801 ccgcgtgggg ggtaacggcg ttgttgaagg ccagcgtcgc ggcgtcccct gatcccccca
     1861 cgaccgccac caggaacggg ccgaaggtgg caaagaatgg taccgcgtgc tgcgcactct
     1921 tgaggacctc gatgaagttc ttgatcacgc cggccgcctc caggcccccg atgaacgccc
     1981 cggcggcgat gatgatgccg aagacgaagc cgtaggcgtg gcccatgccg tcgaagaact
     2041 tcgtcacgat ggtgggcgcg ccggtccagg tgaccaccat ccccagggcg gtcccgatca
     2101 tcatggccac gagcaccgtg acccctttcg ggatccaggt gaaggtgctg gtcacccagc
     2161 cgacgttcga gaccagcagg atgaggagcg ggaccgcggg ggtcacggcc atccagatgt
     2221 tcacccgctc cagggcaact ttctcggtct tggcgatctg gcccagattg gccccggcct
     2281 tctcgggggt ccagtcccta tcctgcttga agagctttcc ctccatgaac agcatgacgg
     2341 cgacgaagac cgccaagggg agcgatacgc tcgtatgcgc cgtgatcaca tccatgaccg
     2401 gaacgttggc gatcttggaa agcatggcgt tgtgggcatt gcccgggctc cagatggagc
     2461 cccaggttcc ggcgaagact gacgccgccg ccatgcccgg atgaatgccc gccgccatca
     2521 tggccgggat caggaccacg ccgacggcgg cggtgcagcc ggcggcgctg gccaaggcca
     2581 tattgatgaa gtaggtgacc agcatggccc cgggaatcaa cagcggccgg atcttgatga
     2641 gcggatcggt gagcagcttc accagatgct ggttgcatcc ggtgatctcc atgaccgcgg
     2701 cgaagcccat gacaggggga atggtctgta gcaggccggc cacgaccatg tttctggcga
     2761 aggcatcgga ccaggcctgc ggattccacg cgataacggc catcaccagc ccgccggtga
     2821 gcagggccag gcggacctcc accttcttca cgatggccgc gaacgtgagg accgtgacca
     2881 ccgccgcaat gatgttcatc agcatcgacg cactcctttc tggcccgagg ggatctcgga
     2941 ataatgtggg aatgaccctt ccgtatgaga acagaaaata ttcaggctat gcctgccggt
     3001 gatagaaggc tcctttggcc aggtcgacca cgatcggctc cagtccctgg cgtctgaccg
     3061 ccgccgcccc ggccgacgtg gtgatcatgc ggctgccgtg gccgcacacg taccctcggt
     3121 gcatgatgac ccgaccgttc gccagactca gcaccgcctc ccgccggtcc acgtccaaaa
     3181 ctgtgatgtc ggcgtcagcg cccaagccga agtgcccctt gttgacgagg cccaatatcc
     3241 gtgccgggtt ggtgctggac ttccggacga actcgcccag cgtgagcgct cgcagggcca
     3301 ccagcgccag tcccatgtcc acgatgacgt tccgggggat acctcccccg tcggtgctga
     3361 tacaatccac cacgaaatcc ccggacggcc gcctggccgt ggccagacgc actcggggct
     3421 ccgcaggatt cacggcgaag ctcaccgccg tgtccgtgcc ccgctgccgc cagaaatccc
     3481 gggcggccgc gccggttgcc aggatcacct ggccgcccgc ctccaggttg atctgggccc
     3541 agccggccag gatggcctcc tccatgcccg ccgtggtggc atcgaaccct ccccgccgca
     3601 ggcactgctg cgtcacccga ctctcggggg cgcccccaac gcacttcgcg ctcgtcccat
     3661 tcagcggcga cagatagctc tcggagcaga gatggggatg ctgctccaac gcgacgatcg
     3721 cctcttccgt ctcctgcatg tagggcctga cggccccgcg gcagtagctg ttgacgtggg
     3781 cgatgtggag ggcgttgccg gcggccaggt caaccgcctg caggaacccg tcaatgttcg
     3841 aaccgtgctc cagggtcccg acgtgaaacc cgacatatgc ccgtctctgg ttcgcgacct
     3901 cgatcgccct ggccgtggca tccggtgtga gggggtagtg cccgccgagg agcttgagac
     3961 caatcgctcc ccgctggagc gcggcctcca ggaccgcctc caactcctcc cggccggggt
     4021 tcgtatctcg gaccgtccct cccggtcgca ccgcctcgat gcaggcgatg ttcatgcccg
     4081 cgccgtggtc ccgggccatg cccatgacgc tgtccacggg tccagacata tccagggcgg
     4141 tcgtcacacc gacctgcgcc atcatcctgt ggccgcactt ccccccgagc cactcggaga
     4201 catgggtatg cagatcgatg acgcccggcg ccacgcagaa gccgcggagg tcgaagacct
     4261 cgcccgcctg cgtgggatcg atctcgggtg ccagctcggc gatcttgccg tcggccaccg
     4321 ccaggtcgga cacgccatcc cgcgccgtag ccgggtccac caccctgccg ttccgcagca
     4381 gcacgtcgta ccggggaccc atggttgccc tctccattcg ccagtgtagc cgggggagtc
     4441 gccgggtgga ttgtctacaa tcgatcccgc cggaaggcgt tcccctcccc cacgagcccg
     4501 ttggggaggt ggactttgcg cgctggatcc gcaaccgccc tacccaggta gaaagcctcg
     4561 acgccgagat ccttcagggc ggccaggatt cgatcggcct gggcggcggc gaccatcaag
     4621 cagaaaccaa ctcccatgtt atagacctgg aacatctcct cgtcggtcac ttcccctagc
     4681 cgctgcagca ggctgaagat cggctggggg gcgggcaggc tgtccagaac atagccgacc
     4741 ttggcctcca cgcgggccag gttgagcagg ccgtcgctgg tgatgtgagc catggcccgg
     4801 atatcgaagc cggcgtgcag cagtgccagg acctcgcgga cgtagatgcg ggtgggctcc
     4861 aagagttcct cgccgatgct gcggcccagt tcaggcacgt gccggtcaac tgcgtacccg
     4921 gcgcgccgga agaatatttc ccgggccaga gtcaggccgt tgctgtggat cccgctgctg
     4981 cggagaccga tgacggcatc tccaggctcg atggtccggc cgatgatgat ccggtccatg
     5041 gggacggtgc cgacgcaggt gccggccaga tcaaagccgc ggccctcccg gaccccccgg
     5101 atgagctccc ggacctgggc gatctcgccg ccggggatgg agatgttggc ctgccgcgcc
     5161 ccctcgtaga gaccctttcc gatggccgtg agcagccccg gatcggcctc ctggacggcg
     5221 atgtaatcga gcatgcagat cggctcggcg cccacgcaga ggaggtcgtt cacgttcatg
     5281 gcgatacagt cgatgccgac ggtgtcgtac ttgtccagca tctcggccac gagcagcttg
     5341 gtgccgaccc cgtcgctgga gagggccagg ccgaggttgt tccccaggtc caggacggtg
     5401 gcgaagtacc cgaagtccag ggcggagcgg taccgtccga aggccagggt ccggccgacc
     5461 cacgtgagga ggccggccag tgccgcctcc tccttgatcg tgttcacccc cgcctgggtg
     5521 tagttgacct cgctcattct gacctcgttt cctcgccggc cccttgcccg ttgccctatg
     5581 ccctttgccc tgtgccattt gccctgtgct gtgccctgtg caccttgccc tgtgcccgtc
     5641 ggccctcact tcaccttcga gccggggact gcctgccgct cgggagtcac caggaccagc
     5701 cggccgtccg ccccggacgc agccaggagc atcccccggg actcgacccc catcagcttg
     5761 gtcggcttga ggttggccac gatgacgatc tgcttcccca ccagttcggc tggtggataa
     5821 tcgatgagga tcccggccac gatggtccgc tcctccgagc ccgtggcgat cctgagcttg
     5881 accagctttt tcgacccggg aaccgcctcg gccgtgagga cctgcgccac gcggagatcc
     5941 agtctgccga actcctcgat gctgatctcc tcgccagtcc cctgtgccga agcgggggtg
     6001 gctgatttcg gcgcctcctc cagggagaat tccacgcggg gaaagagggc ctcgcccttg
     6061 gccatggctc tcacaccgcc gacaccaaag
//
LOCUS       DBNGZC010000760         5353 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1728046, whole genome shotgun sequence.
ACCESSION   DBNGZC010000760 DBNGZC010000000
VERSION     DBNGZC010000760.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1728046"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1523)
                     /locus_tag="AC16G0_13055"
     CDS             complement(<1..1523)
                     /locus_tag="AC16G0_13055"
                     /inference="COORDINATES: protein motif:HMM:NF013206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR5100579.1"
                     /translation="MNRIVVCPEPLAAAAGAEIFRAGGSAVDAALATAFAQAVVSPGM
                     TTIAATGVMNLYHAPSRRNVLIDFLDRAGSSARPDAFAGTRPGEILFGYGSILVPTFV
                     RGTQLAFERFGSGRIAWHELLEPAIRYAEEGFTVYPYLHQYWRADNPVLQTTSPFDGY
                     HMLSTTPACAAIFTREGRVPSIGERLIQPDLGRTLRRLASEGPDEFYTGETGRRMAAD
                     LERHGAPVTERDLTLCRAEIREPLCGSYRGLRISTDPPPAIGTLLLELLNILEGYDLR
                     ALGQESADYYDLLARALFLVFADRTRLAEPGAGPALFPELTSKAHAAELCRRIGEVPR
                     RPRQAPSSLAGTTHVTTYDDQGSAVGMTHTVCYGSGVVTPGLGFMYNNGMCMFDPRPG
                     QPNSIAPGKRPVAGGGPAILFQGDGVRLVLGSPHGGRKTSSMAHVLASLLDFGLSAAA
                     AVASPRIHCEHDPRELRVDPFFPLDTRRDLERFGYLVREDPYGGRVCLVAVDPETQKA
                     "
     gene            complement(1551..2999)
                     /locus_tag="AC16G0_13060"
     CDS             complement(1551..2999)
                     /locus_tag="AC16G0_13060"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5100580.1"
                     /translation="MSRDAVSRYIETHLDSHVRKIQDLVRQPSVSLERQGLRECAELV
                     RRHLAELGCQQTALIDVGDAYPGVWARLDSGAAKTILYYSHYDVRPVGQERWDWPPFS
                     ADIVEMPPYKRVIVGRGARGMKGPLQAWLNALQAIKVVEGKLPVNILFLIEGAEILGS
                     CNYLQLAEAYREQLREADALLSPGASQNAQGEVSVVLGYKGLLYLELEASGEAWGRGP
                     SAPVHSATTAVVDSPVWRLVHALATLTDREGQRLVVDGLEPLFDRRKDISAEERDLLE
                     RLHRRFAGRDPNTVIPGLSAGAPVKVFKRGLSGQAVLEEYLYGPSVNISGLRSGYTGP
                     GSKSFLLPHQAVATLDIRLITDLDAQEVLRLLRAHLERHGFGDIRVHVYGAYNWGQTD
                     PRADVARATLETLESHGYPAVVWPMVAFGGPWAHLPKALGIPALRGAGPGYGDRMATS
                     NEFYVIEGDGTVADLADTEKFYVDLLYTYATL"
     gene            3673..4653
                     /locus_tag="AC16G0_13065"
     CDS             3673..4653
                     /locus_tag="AC16G0_13065"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR5100581.1"
                     /translation="MMRRIGVAAISMLLCLVVLTGTPSGAWSQEKYPSRAIDIIVPFN
                     PGGSTDISTRMLTDFLKTKWGIAVNVINKPGGATVPANLEVYKARPDGYTLFADNTSS
                     NVTLEIAIKDLPFKVMDRTFVAMHSASPYIITVPAASPYATLKDLLDDFKKDPEKFTY
                     VSAGALANHDIIYRQVAHALGIDPAKAKPIVISGAAQAAVVTAQNSVKMGGGTVSSQL
                     VAIKAGTIRAVAVAGKDRWPDLPNVPSTAELGYPTVQADQWNGISGPPKMSAAIVKTW
                     ENYIQEMAKDASVVSKLKTVGAWGRYMGSEDLTKYVQNTAAEMRTLWEVK"
     gene            4813..>5353
                     /locus_tag="AC16G0_13070"
     CDS             4813..>5353
                     /locus_tag="AC16G0_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100582.1"
                     /translation="MPAFFALAVEAPSSGAAILLGVGAVSQSGEKVCGRRSLLRRQPL
                     AGSAHIFRGTPMNRSLITEALSLFVLSALGIYGGLALFLNRDVRTQSSVLAPGIYVLA
                     LGGLLFLTALVYMCLASRRASRIPPASVGRRVAWVSPIVLKMVGAFALYAYLIDLVGY
                     SAPTVLFLLTEFRLLGVRSW"
BASE COUNT          923 a         1762 c         1715 g          953 t
ORIGIN      
        1 gccttctgcg tctccggatc cactgccacc agacagactc gcccgccgta ggggtcctcg
       61 cgcaccaggt aaccgaaccg ttcgaggtcg cgtcgggtgt ccagaggaaa gaacggatcc
      121 acccggagct cccgaggatc gtgctcgcag tgaatgcggg ggctggcgac cgccgccgcc
      181 gccgaaaggc cgaagtccag caggctggcc aggacgtggg ccatggagct tgtctttcgc
      241 cctccatgcg gtgagccaag gactagccgc accccatcgc cctggaacag gatggcgggg
      301 ccgccgcccg ccaccgggcg cttgccgggc gcgatagaat tcggctgtcc cggacggggg
      361 tcgaacatgc acatgccgtt gttgtacatg aaacccagac ccggcgtcac cacacccgag
      421 ccgtagcaga cggtgtgcgt catgcccacg gccgagcctt ggtcgtcgta cgttgtgaca
      481 tgggtggtgc cggccaggct cgagggtgcc tgccgcggcc gacggggaac ctcaccaatc
      541 ctgcggcata gctccgcggc gtgggccttg gaggtgagct ctgggaacag ggcggggccg
      601 gcgccgggtt cggccagccg ggtccgatcg gcaaagacca ggaacagcgc ccgggccagg
      661 aggtcgtagt agtcggcgct ttcctgtccg agggcacgca ggtcataccc ctccaggata
      721 ttcagcagct ccagcagcaa ggtgccaatg gccgggggag gatcggtgct gatgcggagc
      781 ccgcggtagc tgccgcaaag cggctcccgg atctcggccc gacacagggt cagatcgcgc
      841 tccgtcaccg gtgccccgtg gcgctccagg tccgccgcca tcctgcgacc ggtctccccc
      901 gtgtagaatt cgtcggggcc ctcgctggcg agccgccgga gcgtgcgccc caggtcgggc
      961 tggatcagcc gctccccgat gctgggcacc cgcccctccc gggtgaagat tgccgcgcag
     1021 gcgggagtgg tcgagagcat gtggtaccca tcgaagggag atgtggtctg caggacgggg
     1081 ttatccgccc gccagtactg gtgcaggtag ggatagaccg tgaacccttc ctcggcgtag
     1141 cggatggcag gctcaagcaa ttcgtgccac gcgatgcggc cgctgccgaa tcgctcgaag
     1201 gcgagctggg tcccccggac gaaggtgggg acaaggatgg acccgtagcc gaagaggatc
     1261 tccccgggac gggtgcccgc gaaggcgtcc ggacgggccg aggagccggc ccggtccaga
     1321 aaatcgatga ggacgttccg ccggctcggc gcgtgataca gattcatgac gcccgtggcg
     1381 gcgatggtcg tcatgcccgg cgagacgacc gcctgggcaa aggcggtggc cagcgcggcg
     1441 tccaccgcgg agcccccggc gcggaagatc tccgcaccgg ccgccgccgc caagggctcc
     1501 ggacagacca cgatccggtt caccgcgacc tcctccggac ggcccccgca ctagagcgtc
     1561 gcataggtgt acagcaagtc cacgtagaac ttctccgtgt ccgccaggtc agccaccgtg
     1621 ccgtcaccct cgatgacgta gaattcgttg ctggtggcca tgcggtcccc atatcccggg
     1681 ccggcgcccc gcagggccgg gatccccaac gccttgggaa ggtgggccca tggcccgcca
     1741 aaggccacca tcggccagac caccgcaggg tacccgtggc tctccagggt ctcgagtgtg
     1801 gccctcgcca cgtccgcgcg aggatccgtt tgcccccagt tgtacgcacc gtagacgtgc
     1861 acgcgaatat cgccgaaccc gtggcgctcg aggtgcgccc tcaggaggcg gagcacctcc
     1921 tgagcgtcca gatcggtgat caagcggatg tcgagggtgg caaccgcctg atggggcagg
     1981 aggaaggact tgctgccggg gccagtgtag ccgctgcgca ggccgctgat gttgacggag
     2041 ggcccgtaga gatactcctc cagaacggcc tgcccgctca ggccgcgctt gaataccttc
     2101 acgggggcgc cggcggacag accaggtatg actgtatttg gatcgcggcc cgcgaaccgg
     2161 cggtgaagcc gttccagcag atctctttcc tcggcgctga tatccttgcg gcgatcgaag
     2221 agcggctcca gcccgtccac gaccaggcgc tgaccttccc gatccgtgag tgtcgcgagg
     2281 gcgtgtacca ggcgccagac gggactgtcc accacggcag tggtggcact gtgcaccggg
     2341 gcgctgggtc cgcgccccca cgcctccccg ctggcctcca gctccagata gagcaacccc
     2401 ttgtacccga ggaccacgga cacttcaccc tgggcattct gcgaggcgcc ggggctgagg
     2461 agggcgtcgg cctcccgcaa ttgctcccgg tacgcctcgg cgagctgcag gtaattgcag
     2521 cttcccagga tctccgctcc ttcgatcagg aacaggatat tgactggtag cttcccctcc
     2581 acgaccttga tggcctgcaa ggcgttcagc caggcctgca gcggaccctt catcccccgg
     2641 gcgccccggc cgacgatcac ccgcttgtac ggtggcatct cgactatgtc cgcgctgaag
     2701 ggcggccaat cccagcgttc ttggccgacg ggccggacat catagtgcga gtagtacagg
     2761 atggtctttg cggccccgct gtccaggcgc gcccagactc ccgggtaggc gtcgcccacg
     2821 tcgatcagcg cggtctgctg acagcccagc tcggcgagat gccgccgcac cagctcagcg
     2881 cactcgcgga gtccttgcct ctccaggctg acgctcggct ggcgcacgag gtcctggatc
     2941 ttccgcacgt gggaatccag gtgagtctcg atgtaccgcg agaccgcgtc tcgactcatt
     3001 attccccctt tctgcggagc gacccgggaa cagcccagtc gccgtttgtg gcttccttgg
     3061 gaggtgtgag gggggtgagc agccgcgcca cctggtgatc acgttcggga gcgcgatggg
     3121 tcaccatgga atggactata ggccgacaac acagctcttc gcaatctgaa cttccaggac
     3181 gctcatcgaa gcccaccgcc ctttaccctt tgccctgcgc tccgcccttc acccctccgc
     3241 aattcacaat tcacaattcg aaattgcccc aggtgggctg tgccgtttac ctgtcctccc
     3301 caggcgacat ttccctcatg cgccttgcgt ccttgtcggc actttgatat accacgccca
     3361 catctttgtt ttggcctcgc aaggcggagg ggcctgaaga ccgaacacac ccatttctcc
     3421 gatgcgccgt ttccccggtt cccgtgtccg gtttcgccag gcagcccccg ttgtcgaggg
     3481 tcacacggat cctgatctgg agtactcctc ttccgcactt cccctgaccg tggtcgatga
     3541 tgctggggta gcggtgggga ctgacgagac gacgggcacc cgccaagcga tgcacacggc
     3601 tcagcggacg cgtgggcggc tggtgccagg catgcggtca acctctggca gtgagagaag
     3661 gaggtagcgt ccatgatgcg gaggatcggt gtagctgcta tttcgatgct gctctgtctc
     3721 gtcgtgttga cgggaacgcc gagcggcgcc tggtcgcagg agaagtaccc gtccagagcg
     3781 attgatatca tcgttccctt caatcccggc ggctcgactg acatctccac ccgtatgctg
     3841 actgatttct tgaagacaaa gtggggcatc gccgtgaacg tcatcaacaa gccgggagga
     3901 gcgacggttc ccgccaatct ggaggtctac aaggccagac cggatgggta tacgttgttc
     3961 gcagacaata cctcttcgaa cgttacgctc gaaatcgcca tcaaggacct gccgttcaag
     4021 gtcatggacc gcaccttcgt tgcgatgcat tcggcgagtc cgtacatcat cactgtaccg
     4081 gccgcatcgc cgtatgccac cctcaaggac ctgctggatg atttcaagaa ggatccggag
     4141 aagttcacct atgtgtcggc cggcgccttg gccaaccacg acatcatcta ccgtcaagtc
     4201 gcgcacgccc tcggcatcga tcccgcaaaa gcaaaaccca tcgtgatcag cggcgcagcc
     4261 caggcagccg tcgtcaccgc tcaaaacagc gtgaagatgg gcggcgggac cgtgagctcg
     4321 cagctcgtgg cgatcaaggc aggaacgatc cgcgcggtgg ccgtcgccgg caaggataga
     4381 tggccggatc tgccgaacgt gccctcgacg gcggagcttg gctacccgac cgtgcaggcg
     4441 gaccagtgga acggaatctc cggccccccg aagatgtctg cggctatcgt caagacctgg
     4501 gagaactata ttcaggaaat ggccaaggat gccagcgtcg tctccaagct gaagactgtg
     4561 ggtgcctggg gaaggtacat gggttccgag gacctcacaa agtacgtgca gaataccgcc
     4621 gcggagatgc gaaccttgtg ggaagtgaag tagcgcggct cgagtccaac cacctgatcg
     4681 catccggggc atcctggctc tccttgggag tgggcgcgga cggctcttcc gcagacaccg
     4741 tcgctgcggc acgcgtgaga gcacctcgcg gcgaccggga cttccagcgt cgagccatcc
     4801 cccggcggcg gcatgcccgc ctttttcgcg ctagcagtag aggctccgtc gtcaggcgct
     4861 gcgatccttc taggtgttgg cgcagtttct cagagcggcg agaaggtttg cggtcggcgc
     4921 tccctgctgc gcagacagcc cttggcgggg agcgcacaca ttttcagagg cacgccgatg
     4981 aaccgtagcc tgatcaccga ggcattgagt ctcttcgttc tcagtgccct tgggatctat
     5041 gggggacttg cgctatttct caacagagat gtgcgcactc agtcgtcggt gctagccccc
     5101 ggcatttacg tccttgccct cggtggcctg ctctttctca ccgcgctcgt gtacatgtgc
     5161 ctcgcctcca ggagggcctc tcggatcccc cccgcttccg tcgggcggcg cgttgcctgg
     5221 gtgagcccga tcgtgctcaa aatggtcggc gcctttgccc tttacgccta tctgatcgac
     5281 ttggtcgggt acagcgcgcc cacggtactc tttcttctga cagagttcag gctgcttgga
     5341 gtcaggtcct gga
//
LOCUS       DBNGZC010000761         1228 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2218818, whole genome shotgun sequence.
ACCESSION   DBNGZC010000761 DBNGZC010000000
VERSION     DBNGZC010000761.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2218818"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="AC16G0_13075"
     CDS             <1..557
                     /locus_tag="AC16G0_13075"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR5100583.1"
                     /translation="NIVSYTTTIEQGWGTGIMVPGYGFLLNNELTDFNMVPTFNSTTG
                     NPGANDVAGNKRPRSSMTPTIVLKDDKPFAAYGSPGGATIINTVFDITLDLIDHGMTI
                     QQAINAPRISVTGAGGSVSMESGFSPAVINALKALGHKSISTGGVQGSVQAVIIDLQT
                     GKQYGGADSRREGTVIGLPRPGGK"
     gene            818..>1228
                     /locus_tag="AC16G0_13080"
     CDS             818..>1228
                     /locus_tag="AC16G0_13080"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR5100584.1"
                     /translation="MEYRSAIAAANQRFMEAFRRGDAAGLANLYTADGQILPPYHNII
                     TGRTNIQAFWQGLLDTGLKTATLQTTEAKANGHFAVEVGRYTFLDADGRLRDAGKYVV
                     VWRRDMDSWLLHQDIWNTDLATSAKSTDATSKASP"
BASE COUNT          258 a          373 c          400 g          197 t
ORIGIN      
        1 gaaacatcgt cagctacacg acgacgatcg agcagggctg ggggacgggc atcatggtgc
       61 ccggctacgg attcctgctc aataacgagc tgaccgactt caacatggtg ccgacgttca
      121 actccaccac ggggaacccg ggcgccaacg atgtggccgg caacaagcgc ccccgaagca
      181 gcatgacgcc caccatcgtc ctcaaagacg acaagccgtt cgcggcctat gggtccccgg
      241 gtggcgccac gatcatcaat acagtgttcg acatcacgct ggacctgatc gaccacggca
      301 tgacgatcca gcaagccatc aatgcgccgc ggatctccgt cacgggtgcg ggaggatctg
      361 tgagcatgga atcggggttc tcgcctgccg tgatcaacgc gctcaaggct cttgggcaca
      421 agtctatctc caccggcggc gtgcagggct cggtgcaggc ggtgatcatc gatctccaga
      481 cagggaagca atacggtggc gcggactcgc gccgggaggg gacagtcatc ggactcccga
      541 gaccgggagg aaagtaaccc attggttgcg ggggggccac gcggccgccc cgcaactgcc
      601 tgaagctcct acgggaggtg ggctggcggc gggaggtatg tgagcggctg gctccggttg
      661 cggctgcccg tctgaagaac ggagagcctc gctgaggcgg agggcgaggc ggtggcctcc
      721 cctgcgcaag ggcgatggcc ttcctcggaa gcggagtagc gtgatcggac gcgaagccga
      781 aattccgcac tggtaaaagg agtcaatgaa ttcactggtg gagtatcgtt cggcaatcgc
      841 tgcagcaaac cagcggttca tggaggcctt tcggcgaggg gatgccgccg gcctggcgaa
      901 tctctacacg gccgatggtc aaattctccc cccgtaccac aatatcatca ccggccggac
      961 gaatatccag gcgttctggc aagggctcct ggacacgggt ctcaagaccg ccacgctcca
     1021 gacgaccgag gccaaagcca acggccattt cgccgtcgag gtcgggcgat acacctttct
     1081 cgacgcagac ggccggcttc gggatgctgg aaagtacgtc gtggtctggc gaagggacat
     1141 ggacagttgg ctgctccatc aggatatctg gaataccgac ctcgcgacat cagcgaagtc
     1201 gacggacgcc acctccaagg caagcccg
//
LOCUS       DBNGZC010000762         1531 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1828790, whole genome shotgun sequence.
ACCESSION   DBNGZC010000762 DBNGZC010000000
VERSION     DBNGZC010000762.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1828790"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1020
                     /locus_tag="AC16G0_13085"
     CDS             127..1020
                     /locus_tag="AC16G0_13085"
                     /inference="COORDINATES: protein motif:HMM:NF048886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkZ-related protein"
                     /protein_id="HIR5100585.1"
                     /translation="MSPDKPRHDITTRQEVEAWRDHAFRRLPELRIRGEKSALHFVDE
                     VGFCFTLSDFGLPVASLYVAVCGRRHPRWPKRTHHDPEIGLTWSLKDSLPAGRLTYYG
                     KLVKGKPTLVSLRLLPAFCALIRDGKGSGDYILDYRQGRMTRAAVAILEALHERSPLT
                     TPELRKLLAMHAPDQTAEFDRAMAELQRGMWMVKVEEVYDPDFYYRWDLLDNWLPGPV
                     KASLELSRAEAVRQLVGTYLRGAAASQPRFLAGLFGLSAGEVEAALASLEAEDGLRRN
                     ARIKGLPGAWIVWLGRADRRH"
     gene            1001..>1531
                     /locus_tag="AC16G0_13090"
     CDS             1001..>1531
                     /locus_tag="AC16G0_13090"
                     /inference="COORDINATES: protein motif:HMM:NF016163.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HIR5100586.1"
                     /translation="MRIVGIDLAWGDRKPDGLALIDAGGDGRATLRRTACVFGDEALV
                     SWVREYAGIGPALLALDGPILCQDLTGARPVDRETHRRFGRAHAGCYPANLSRCPRPN
                     RIRLRLEALGFRTDYRMRGPRQATEETKGPLRRLIEVYPHPATLALFDLPHIIRYKKG
                     PAAERARELQRFQRLLH"
BASE COUNT          241 a          496 c          531 g          263 t
ORIGIN      
        1 gaggagctga gcgaacccgc acctactttc tctgcttgtc ctttcccctg ttcgttggat
       61 agtatgacgt ggcgtcggct ccaggattcc ccgaggcagc gtgcccacct ggggagagga
      121 ccgggaatgt ctccagacaa gccacggcat gacatcacga cgcgccagga ggtcgaagcc
      181 tggcgcgatc acgccttccg ccgcctgccg gaactcagga ttcgcggcga aaagagcgcc
      241 ctccatttcg tcgacgaggt cggcttctgc ttcacgctga gcgacttcgg cctgcccgtg
      301 gcgagcctct acgtggcggt ctgcggccga cgccatcccc gctggcccaa gcgtacccat
      361 cacgaccccg agatcgggct cacctggagc ctgaaggaca gcctgccggc cggtcgcctc
      421 acctactacg gaaagctggt gaagggcaag ccgaccctgg tctcgctccg cctgttgccg
      481 gccttctgcg ccttgatccg tgacgggaag ggcagcgggg attacattct ggactaccgg
      541 cagggccgga tgacccgcgc ggccgtggcc atcctggagg ccctgcacga gagaagcccc
      601 ctgaccacgc ctgagctgcg caagctactg gccatgcacg ctcccgatca gactgccgag
      661 ttcgaccggg ccatggccga actccagcgc gggatgtgga tggtgaaggt ggaggaggtg
      721 tacgacccgg acttctatta ccggtgggac ctcctggaca actggctgcc ggggccggtc
      781 aaggcctctc tcgaattgtc gagggccgag gcggtgaggc agctcgtggg cacttatctc
      841 cgaggtgcgg ccgcgtctca gccccggttc ctggcgggtc tcttcggtct ttcggcgggg
      901 gaggtcgagg ccgctctcgc ctcgttggag gcggaggatg gcctccgccg gaatgcgcgc
      961 atcaagggcc tgccgggggc ctggatcgtg tggctgggcc gtgcggatcg tcggcattga
     1021 cctggcctgg ggagaccgga agccggatgg cctggcccta atcgatgcgg gcggcgatgg
     1081 ccgagccact ctccgacgga ccgcatgcgt cttcggcgac gaggccctgg tttcctgggt
     1141 acgagaatac gcaggaatcg ggccggccct cctggcgctg gacggaccta tcctctgcca
     1201 ggatcttacg ggcgctcgtc ccgtggaccg ggagacgcac cgccgctttg gccgcgccca
     1261 tgcggggtgc tacccggcaa acctgagccg gtgcccgcgg ccgaatcgaa tccgccttcg
     1321 tctggaggcg ctgggcttcc ggactgatta ccggatgcgg gggccgaggc aggcgacgga
     1381 ggagacaaag ggaccgttgc gtcgtctgat agaggtgtac ccgcacccgg ccacattggc
     1441 gctgttcgat ctgcctcaca tcatccggta caagaagggg ccggcggcgg agcgggcgcg
     1501 ggagctgcag agattccagc gtctgctgca c
//
LOCUS       DBNGZC010000763         4244 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1242013, whole genome shotgun sequence.
ACCESSION   DBNGZC010000763 DBNGZC010000000
VERSION     DBNGZC010000763.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1242013"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="AC16G0_13095"
     CDS             complement(<1..605)
                     /locus_tag="AC16G0_13095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100587.1"
                     /translation="MNDLGCRVLSLTTLALAALLFMAQPAPAVAADEISNTHFPKPAA
                     LQANVEFWKQIYTDYGVGDFVLHDRENLGIIYDVVRIQDTTNECRAAAAAKPEIQRLR
                     GKYADILEKLAQGVAPEELGPEGSRVAQAWGCPCSPEVLRRGAGNIRAQQGMREKVQD
                     GMKRARGLMPPILSVLRRHNVPEELAALPMVESSFNPRAQS"
     gene            1070..2950
                     /locus_tag="AC16G0_13100"
     CDS             1070..2950
                     /locus_tag="AC16G0_13100"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5100588.1"
                     /translation="MNLRTRLLISYMIFVGTLAAVGGWSAWHLHQTGQVTEHIIADNF
                     DDVIAVQEMMASIGRQDSAVLLARLGQRERARERLAQYRHLFDVSFRAAEKQITEPGE
                     AEIMESIRRGREAYYREVDALLEEIDRGGGAFLQGPEALGRNERFLRQETLFNQLRAA
                     CMRFLQLHDHAIISKSAAAARATRVWFFATIGLVASLVLSGIALAIFLSSRIVQPVRE
                     LTATAGKIAGGDLEVEARVDTQDEVGILAAEFNRMAERIRELRRSDMGKLIVAQQTTE
                     AAIDSLYDPVIVTDAHGMVTKLNPAAEELFGPETQNKGRPVGDIAPASRIAVAVAETL
                     SSQRPVAKEDAASVLSLCVDGSERAFRLRTTPMRDEDGRLLGAVTLLQDITHLREIDR
                     VKSEFIATASHELQTPLTSLHMGVHLLLEGAAGALTDKQRGVLQACHEDCGRLEELMR
                     DLLDLSKIEAGARTPRLRAIRVSELIATTAESFRAQAEAKGITLVVEASSDLPSALAD
                     RLQIERVVSNLVSNALRHTGAGGEIRISAVLRNGHVTIAVMDTGQGIPPEHLPHIFDK
                     FVQVPNAPSGRAGLGLAISKRIVEAHGGQILVQSTVGQGTTFTFTLPVASERLAGSPV
                     EG"
     gene            2961..3740
                     /locus_tag="AC16G0_13105"
     CDS             2961..3740
                     /locus_tag="AC16G0_13105"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5100589.1"
                     /translation="MGKRILIVDDEDHIRQMMRLTLETAGYEVGEAGDGLQGLANFGD
                     GSGWDAVVLDQRMPGLDGLETLRRIRERNASVRVIMVTAYASVELAVDAMKVGATDFV
                     RKPMTPEILRNAVVAAVTKPVAAPAPTSEPTTEAAIEHPYPSATRLTFINGFRSWHAP
                     DVTVAPPLEPNQRHFIVQHPEGWQREVVVEIDPEAIGYVQREAHRPIPADDEFWTTQA
                     AHFLNAFLWDMGRVPPTGKLTIDVHGIDRDVLQMATRWGSD"
     gene            complement(3880..>4244)
                     /locus_tag="AC16G0_13110"
     CDS             complement(3880..>4244)
                     /locus_tag="AC16G0_13110"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6879 family protein"
                     /protein_id="HIR5100590.1"
                     /translation="TIDIAVYNFTAAELAKALYRAKARGVQIRILVDRENAEAGFIIR
                     ELRRHDLPLRTLGVPEQSLMHHKFAVFDDRHVATGSYNWTQSAEHANYENLVILDDPE
                     AVTRYQREFNRLWREAKE"
BASE COUNT          785 a         1315 c         1368 g          776 t
ORIGIN      
        1 ttggactggg cgcgaggatt gaagcttgac tctaccatgg ggagggccgc caattcctca
       61 gggacgttgt gccgccgaag gacggacaag atgggcggca tcaagccacg ggcccgcttc
      121 atgccatcct ggaccttctc acgcatgccc tgttgcgccc ggatgttgcc cgctccccgg
      181 cggaggacct ccggcgagca tgggcaaccc cacgcctggg ccacacgcga tccctcgggc
      241 cccaattctt cgggggctac gccctgggcc aacttctcga ggatgtcagc atacttgccc
      301 cggagccgtt ggatctcggg cttggcggct gcggcggcgc ggcattcgtt tgtcgtgtcc
      361 tggatgcgca ccacgtcgta gataatgccc aaattctccc gatcgtggag gacgaagtcg
      421 cccacgccgt agtcggtgta gatctgcttc cagaattcga cgttggcctg aagggctgcc
      481 ggcttgggga aatgggtgtt tgaaatctcg tctgctgcca cagccggggc aggctgggcc
      541 atgaagagaa gagcagcgag ggccaaagtc gtcagcgaga ggacacggca gccgaggtcg
      601 ttcattctcc ctccgccccc acgcaaccga ttcacatgcg gcaaccttca cttgttcttt
      661 atctagcaga ccattttgtg gagtgcaaga aattttcgaa tcgacaccgc tacatgcttt
      721 tcgtagcgaa tgactcgacg gcacttctag ggctgctttt gccttgacag gatcgaatgc
      781 aatgttactg tttgccaatc ttgacagtgc cctgcctcag ctcccgggga aacagcatga
      841 tgaccgacgg cgagtgctgg ccccagcccc tggaacctat tcccacgccg ccttcgccgg
      901 atggtgccat ttgtatggag agatggagag gtgatgactc cagtcaggct atagttcttt
      961 accgattctg tggcttgccg cccatgggct gtcaagtcgc ccgccaggcg gtggcgtggt
     1021 gacggcggtc gaggctggag atgacgcgac gtgagctaag ggacgcggga tgaacctccg
     1081 caccagacta ctcatcagct acatgatctt cgtgggcacg ctggccgcgg tagggggctg
     1141 gagcgcttgg cacctccacc agaccggcca agtgacggag cacatcatcg cggacaactt
     1201 cgacgacgtc atcgccgtcc aggagatgat ggccagcatc ggccggcagg attcggcggt
     1261 cctcctggct cgtctcggtc agcgggagcg ggcccgggaa aggttggcgc agtaccggca
     1321 tctgtttgat gtgagcttcc gcgccgcaga aaaacagatt acagaaccgg gcgaggccga
     1381 gatcatggag tccatccgtc gcggacgcga ggcgtattac cgggaggtgg atgcgctgct
     1441 ggaggagata gacagggggg gcggtgcctt cctgcagggg cctgaggccc ttggtcgaaa
     1501 cgagcggttc ctgcggcagg agaccctctt caatcaattg cgcgccgcgt gcatgcgctt
     1561 tctgcagctt catgatcacg cgatcatttc aaagtctgcg gctgccgccc gggccacgcg
     1621 ggtgtggttc ttcgccacca tcggcctggt cgcctcgctg gtcctctcgg gcattgccct
     1681 ggccatcttt ctctctagcc ggatcgtgca gccggttcgt gagttgaccg cgacagccgg
     1741 aaaaatcgcc gggggcgacc tcgaggtgga ggcccgggtg gacacgcagg atgaagtggg
     1801 catccttgcc gccgaattca accgtatggc cgagcgcatc cgcgagctac gccgctccga
     1861 catggggaag ctgatcgtcg cgcaacagac gacggaagct gccatcgact ccctgtacga
     1921 tcccgtcatc gtcacggacg cgcacgggat ggtcacgaag ctcaacccgg ccgccgagga
     1981 gttgttcgga ccggagaccc agaacaaggg cagacccgtg ggggacatcg ctcccgccag
     2041 ccggatcgcc gtggcggtgg cggaaaccct gagttcgcag cgtccggtcg caaaggagga
     2101 tgccgcctcg gtgctctctc tctgcgtgga cggttcggag cgggcgtttc gcctccgcac
     2161 gactccgatg cgggacgagg acgggcgact ccttggggcc gtgacgctgc tgcaggatat
     2221 cacgcacctg cgggaaatcg accgggtcaa gtcggagttc atcgccaccg cctcccacga
     2281 attgcagacc ccgctcacca gcctccacat gggggtccat ctgctgctgg agggggcggc
     2341 aggcgcgctg accgacaagc aacgcggggt tctgcaggcc tgccacgagg attgcgggag
     2401 actggaggag ctcatgcgcg acctcctcga cctctccaag attgaggccg gggcccgcac
     2461 accgcgcctg cgagccatcc gcgtcagtga actgatcgcg accacggcag aatccttccg
     2521 ggcgcaagcc gaagccaagg gaatcacgct cgtggtcgag gcatcgtcgg acctcccgtc
     2581 cgccctcgcc gatcgcctgc aaatcgagcg cgtcgtcagc aacttggtga gcaacgccct
     2641 gcgccatacc ggcgccgggg gagaaattcg catcagcgcc gtcctccgga acggccacgt
     2701 gaccatcgct gtcatggata cggggcaggg catcccgccg gaacatctcc cgcatatctt
     2761 cgacaagttc gtccaggtgc caaatgcgcc ctccggccgc gccggactgg ggttggccat
     2821 ctccaagcgc atcgtcgagg ctcacggggg gcagatcctc gtccaatcga ctgtcgggca
     2881 gggcacaacg ttcacgttca ccttgccggt tgccagcgaa cggctggcag gatcacccgt
     2941 cgaggggtga ggggggcgac atgggaaaac gcatcctgat cgttgacgat gaagaccaca
     3001 tccgccagat gatgcgcttg acgctggaga ccgccgggta cgaggtgggc gaggcgggcg
     3061 atgggctcca ggggctggca aactttgggg atggctcggg ctgggatgcg gtggtactgg
     3121 atcagaggat gcccggccta gacggtctcg aaacgctccg ccgcatccgg gagcgaaatg
     3181 cgagtgtacg cgtgatcatg gtcacggcct atgcgtctgt tgagcttgcg gtggatgcca
     3241 tgaaggtcgg cgccacggat tttgtgcgta aaccgatgac gcctgagatc ctgcgaaacg
     3301 cggtagttgc agccgtgaca aagccggtgg ctgcccctgc cccgacgtcc gagccgacca
     3361 cggaagccgc catcgaacac ccgtatccat ccgctaccag actgaccttc atcaacggat
     3421 ttcggagctg gcacgctccc gacgtgacgg tggcgccgcc cttggaaccc aaccaacgcc
     3481 acttcatcgt ccagcacccc gagggatggc agcgagaggt cgtggttgaa atcgatccgg
     3541 aggcgatcgg gtacgtccag cgggaggccc accgccccat tccagccgac gacgaattct
     3601 ggacgaccca ggccgcgcat tttctgaatg cgtttctgtg ggacatgggg agggtccccc
     3661 ccaccgggaa actcacgatc gacgtgcatg ggatagaccg ggacgtcctg caaatggcaa
     3721 cacgctgggg cagcgactag gaccaacgga tcaggctaca cgaccgcccg ctctgagatc
     3781 ggatacccga acggtgtata gctcatggct catggatgga agcggcacaa tcggctatag
     3841 gcgctctgct aaattccatg cactactccc catttcacct cactctttcg cctcccgcca
     3901 caatcgattg aactctcgct ggtaccgggt gacggcttcg gggtcgtcca ggatcaccag
     3961 gttctcgtag ttggcatgct cggcgctctg ggtccagttg tagctgccgg tggccacatg
     4021 gcggtcgtcg aagacggcga acttgtgatg catcaggctc tgctccggga cgccgagggt
     4081 gcgcagcggc aggtcatgcc tgcgtagttc ccggatgatg aatcccgcct cggcgttttc
     4141 ccgatccacc aggatccgga tctggacgcc gcgggccttc gcgcggtaga gcgccttggc
     4201 aagctcagcc gcggtgaagt tgtacacggc gatgtcgatc gtcc
//
LOCUS       DBNGZC010000764         5521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2121876, whole genome shotgun sequence.
ACCESSION   DBNGZC010000764 DBNGZC010000000
VERSION     DBNGZC010000764.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2121876"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="AC16G0_13115"
     CDS             <1..222
                     /locus_tag="AC16G0_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehypoxanthine futalosine cyclase"
                     /protein_id="HIR5100591.1"
                     /translation="NIQASWLTQGPKIGQLSLQYGVNDMGSTVIEENVVTAEGAVFMV
                     PMPEIERLIRDAGFTPARRNTRYEILAVA"
     gene            559..1266
                     /locus_tag="AC16G0_13120"
     CDS             559..1266
                     /locus_tag="AC16G0_13120"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR5100592.1"
                     /translation="MALNTLEFSRIGDLAYQAIRDAILSGEFSQGTRLNQDDLARRLG
                     VSRAPIRDALNRLEAEGLVKTLSRAGGVVVAETNEQEMVDIYELRAILDTASTRLACE
                     RMSDDDLRRLQEAVEKTEQVTKAADLNAIVQAHAEFHSLIYAVCGNAELTRVARNLWD
                     RSYRFRVLALSNEENARRGLAQHKAILAALKARDPVQAAVAAEEHDQASILHLRTRMT
                     TRPGDRQNPGPPTAEKS"
     gene            1380..2372
                     /locus_tag="AC16G0_13125"
     CDS             1380..2372
                     /locus_tag="AC16G0_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR5100593.1"
                     /translation="MRNFILALALVAALLPGVASAADWQPTRPVELVVASGAGGGTDI
                     FARTVQSIILKHKLMLQPTVVLNKGGGSGAEGFVYVKSATGDPHKMVFGTNNEWMLPL
                     VAKVGWKYDELNPVAAMAFDEFLLWVRPSAPWKTAGDYIAEAKKRPGEVRMGGSQSKD
                     TDEVLVRMLEKAAGIKLMYVPFKSGGEAAVQLAGSHIDSNTNNPAENVGQWKGGQGTP
                     LCVFSKTRLPAGPKVTATMGWSDIPTCKEQGVPIDESKMPRDVFLPPGVPEAAVAYYA
                     EVFKKVSETPEWAEYIAKTSQSGRYMAPAELKAFIKVDEERGRKLFEEQGWLVK"
     gene            2441..2944
                     /locus_tag="AC16G0_13130"
     CDS             2441..2944
                     /locus_tag="AC16G0_13130"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR5100594.1"
                     /translation="MEQKSTPSVSRLTVESAFALFTCAFGALIIKGALEFNIGWGDIG
                     PEAGYFPFRVGTLIVLASLINLVRALIRRKELATQVFLTREQGRHVVAFAIPVIALVG
                     VTLGLGLYVATALYLLFTVGFIARHRSWVTIVISLGTPLVLFLLFEYVFLTPLLKGPL
                     ENWLGWY"
     gene            2958..4454
                     /locus_tag="AC16G0_13135"
     CDS             2958..4454
                     /locus_tag="AC16G0_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018484235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR5100595.1"
                     /translation="MLENFPLLAQGFALAVSPYNILLMMVGVFLGILVGVLPGLGAPN
                     GVTLLMPLTFGMNPVSAIILLTSMYWGALYGGSTTSILFNIPGEPSSVATTFDGHPMA
                     KAGRATEALTTAFLSSGFGALFGVATVTILSSWATRFALRFSPVEYFTVFMLAFGSFV
                     AFGGGSPIKTIVSIGLGFAWASMGMDTVSGSMRLTYGIPALSRGISLLVVVIGLYGIG
                     ELCQRMQEPMGSVEPVASKLSPREVLRAALVWPRYWIAFLRSACIGVWMGITPGGPTA
                     ATFMSYGIGRRFSRAGARFGTGDPEGLVSSEVSDQAAGTAAMLPMLALGLPSSATAAV
                     LLGGLMIWGLTPGPALFTERPDFVWGLIASMYVSNVVCVVMVLITVPLFAAILRAPFT
                     ITGPLIVIVCLVGTWTVGSAPIDLWMMLAFGAIGYFMSRLQYPVAPLVLAMVLGDRAE
                     DAFRQSMILSHGSILVFWSNPLAGSIATLALLCFAVPGLTALWRRVRQ"
     gene            4812..>5521
                     /locus_tag="AC16G0_13140"
     CDS             4812..>5521
                     /locus_tag="AC16G0_13140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR5100596.1"
                     /translation="MRTKRLGYTDLQLSIIGLGSFAMGGGGWKFSWGPQEDEDSIRAI
                     VRGVELGVNWIDTAPVYGLGHSEEVVGAALKRCSRKPLVATKCGRRWDGDGNILRRLR
                     KASVREEAEASLRRLGVEAIDLYQIHWPEPEEEFEEGWDAMAQLVKEGKVRYLGVSNF
                     SVAQMRRVQAIHPIASLQPPYSLLRRDIESEILPFCSQHHIGVIPYSPMQKGLLTGTF
                     SVERAKNLPPDDHRRNDP"
BASE COUNT          927 a         1717 c         1809 g         1068 t
ORIGIN      
        1 aacatccagg cctcctggct gacccaggga ccaaagatcg ggcagctttc cctccagtac
       61 ggtgtcaacg acatgggcag cacggtgatc gaagagaacg tggtgacggc cgagggggca
      121 gtcttcatgg tccccatgcc cgagatcgag cggctcatca gagacgccgg gttcaccccc
      181 gctcgacgca acacccgcta cgagatcctg gccgtggcct gagggttcgg ggccggatcg
      241 cgccattctc gtgtcccccg actggattcg gccatagttc ccacaccctt cctcgtttcc
      301 cacgcatgct cgggaacctc gatctggcgt ccctgcgtcg agagctggtc ccttccggag
      361 aacggaagca atgaggcgtc cgtccttgct cgtttcactc ctggcttcct gtgagatttg
      421 tcattctctc caattgatct ataagccata caagttactg aaataatcct tgactcattt
      481 catgccccag ttaataatcc tggctacggt atctcgtata caggcgacga tatacttctg
      541 attaaggatc agaattcgat ggcgctcaac acgctagaat ttagtcgtat cggggacttg
      601 gcgtatcagg ccattcgtga tgccatcctc tcgggtgagt tctcgcaagg aactcgcctc
      661 aaccaggatg acttggctcg acgtctcggg gtcagtcggg cgccgatcag ggatgcactc
      721 aatcgcctcg aggccgaggg cttggtcaag actctctcgc gggcgggcgg cgtggtcgtt
      781 gcggagacaa acgagcagga gatggtggac atctacgagc tccgcgccat cctggacacc
      841 gccagcaccc gtctggcgtg cgagcggatg agcgatgacg acctccgccg cctgcaagaa
      901 gccgttgaga aaaccgagca agtgacgaag gcggcggacc tgaacgcgat cgtgcaggcg
      961 catgccgaat ttcactccct catctatgcg gtctgcggga atgcggagct gacgcgggtg
     1021 gcgcgaaatc tctgggatcg ctcgtaccga ttccgggtct tggcgctgag caatgaggag
     1081 aatgcccgcc gcggtctcgc acagcacaag gcgatcctcg ccgctctgaa ggcccgggac
     1141 ccggtgcagg cggccgtggc tgctgaggag catgaccagg cgagtatcct gcacctccgg
     1201 acgcgaatga caacgcggcc aggggatcgg cagaatccag gacccccgac tgcggagaaa
     1261 tcctagatgc aggcttgggg gcgagtttcg gctgggcatc cacagccaag cagcaactgg
     1321 ctgattgcgt tttccctgtg agagtgcagt tggcaaccca aaaaggagga gtgtccccga
     1381 tgagaaactt tattctggcc cttgccctgg tagcggcgct gctgcccggc gtcgcctcag
     1441 ccgccgattg gcaacccact cggcccgtgg agctcgtggt ggcctcaggc gccggcggag
     1501 gtaccgacat ttttgcccgc accgtgcaat ccatcatact gaagcacaag ctcatgttgc
     1561 agccgaccgt ggtcctcaac aagggcggcg gctccggcgc cgagggcttc gtgtacgtca
     1621 agtcggccac aggcgatccg cacaagatgg tgttcggtac caacaacgag tggatgctgc
     1681 cgctggtcgc caaggtcggc tggaagtacg acgagctgaa ccccgttgcc gccatggcct
     1741 tcgacgagtt tctgctctgg gtgcggccca gcgctccctg gaagaccgcg ggcgattaca
     1801 tcgccgaagc caagaagcgg cccggcgagg tccggatggg cggcagccag tcgaaggata
     1861 cggatgaggt cctcgtccgc atgctcgaaa aggctgcggg gatcaagctg atgtacgtgc
     1921 ctttcaagtc gggcggcgag gcggcggtgc aactggccgg ctcccacatc gactcgaaca
     1981 ccaacaatcc ggcggagaac gtcggccagt ggaaaggcgg acaggggacg ccgctgtgtg
     2041 tcttcagcaa gacgcgcctg ccggcagggc ccaaggtgac ggcgaccatg ggctggagcg
     2101 atattccgac ctgcaaggag cagggcgtgc ccatcgacga gtccaagatg ccgcgggacg
     2161 tgttcctgcc gccgggcgtg cccgaggccg cggtggcgta ctacgcggag gtattcaaga
     2221 aggtgagcga gacgccggag tgggccgagt acatcgccaa gacgtcgcag agcggtcggt
     2281 acatggcgcc cgccgaactc aaggctttca tcaaggtcga tgaagagcgg ggccgaaagc
     2341 tcttcgagga gcagggctgg ctcgtgaaat agcggcttcg cacgtgactc cgcctcgcct
     2401 cccgagatgg tccacggggg gcgcggcgag gagcttggga atggaacaga aatcgacccc
     2461 atccgtgtca cgcctcacgg tcgagagcgc gttcgcgctg ttcacatgtg ccttcggtgc
     2521 tctcatcatc aagggcgcgc tggagttcaa catcggttgg ggggacatcg gccccgaagc
     2581 gggctatttt ccgttccgcg tcgggaccct gatcgtcctg gcctcgctca tcaacctcgt
     2641 ccgggccctc atccggcgga aggaactggc gacgcaggtc ttcctgaccc gggagcaggg
     2701 acgccacgtg gtcgccttcg ccatccccgt gatcgcactc gtcggggtga ccctcggcct
     2761 gggtctctat gtcgccacgg cgctctacct cctgttcacg gtcggattca tcgctcggca
     2821 tcggtcgtgg gtgacaatcg tgatttccct cggcacccca ttggttctct tcctgctttt
     2881 cgagtacgtg tttctcacgc cgctcctcaa gggcccgctg gagaactggc tcggctggta
     2941 ttgaggaggg aggggccatg ctcgagaact tcccgctctt ggcgcagggg tttgcccttg
     3001 ccgtgagtcc gtacaacatc ctcctgatga tggtgggcgt gttcctgggg atcctggtcg
     3061 gggtgctgcc cgggctgggg gcccccaacg gcgtcacgct gctcatgcca ctcaccttcg
     3121 gcatgaatcc cgtgtcggcg atcatcctcc tcaccagcat gtattgggga gcgctctacg
     3181 gcggatcgac cacctcgatt cttttcaaca tccccggaga gccctcgtcg gtggcgacga
     3241 cattcgacgg ccaccccatg gccaaggcgg ggcgggccac cgaggcgctg acgaccgcct
     3301 tcctgtcctc tggcttcggg gcgctgttcg gggtggcgac ggtcacgatc ctctcctcgt
     3361 gggccacccg cttcgcgctg cggttctcgc cggtcgagta cttcaccgtc ttcatgctgg
     3421 cgttcggcag cttcgtcgcc tttggcggcg gctctccgat caagacgatt gtctcgatcg
     3481 ggctggggtt cgcgtgggca tccatgggca tggacacggt gtccggctcg atgcggctga
     3541 cctatgggat ccccgcattg agccgcggca tcagcctgct ggtcgtcgtc atcggtctct
     3601 acggaatcgg ggagctctgc cagaggatgc aggagccgat gggctcggtc gagccggtcg
     3661 cttcgaagct gagcccgcgc gaggtcctca gggccgccct ggtttggccc cggtactgga
     3721 tcgcattctt gcgcagcgcc tgcatcggcg tctggatggg gatcacgccc ggcgggccga
     3781 cggccgccac gttcatgagc tacgggatcg gccggcgttt ttccagagcc ggcgcccgct
     3841 ttggcacggg cgaccccgag gggctggtgt cgtccgaggt gtccgatcag gcggcgggaa
     3901 cggccgcaat gctgcccatg ctggcactcg gcctccccag ctctgcgacg gctgccgtgc
     3961 tgctcggggg cttgatgatc tgggggctga cgccgggtcc ggcgctgttc accgagcggc
     4021 cggacttcgt ctgggggctg atcgcctcga tgtacgtctc caacgtggtc tgcgtggtca
     4081 tggtcctgat cacggtgccg ctcttcgccg cgatcctccg cgcgccgttc accatcaccg
     4141 ggccgctcat cgtcatcgtc tgcctcgtcg gcacgtggac tgtgggctcc gcccccatcg
     4201 acctctggat gatgctcgcc tttggggcga tcggctattt catgagccgg ctgcagtacc
     4261 ccgtggcgcc gctggtcctg gccatggtgc tgggcgaccg ggcggaggac gcgttccgcc
     4321 agtccatgat cctttcccat gggtccatcc tggtattctg gtcgaaccct ctcgccgggt
     4381 cgatcgccac gctggcgctc ctgtgtttcg cggtacccgg tctcacagcc ttgtggcgac
     4441 gggtgcggca atagcggacc cggtccggag aaacagcaac gcctgccaga cccgctcccg
     4501 cgggtggttc tcccctcgtg agccctgcca gaggtcgaat gactcccacc cgttgctcca
     4561 ggcgagaagt ccgggcgtgc atctattgca gccaacttcg tctgtggtaa cgggaggtgg
     4621 gtggaattcg aagcggatcc tgtctcttgc cttgccccct caggcttgcg cttctacact
     4681 gcaatccaag tactccaatt gcacgtcgtt gactgggcgg catgcctgct gagcgggccg
     4741 gaatggatgt gccggcgtct tgggcacgcg agttgggccg cggcatcggc taccgcagaa
     4801 aggattcgga gatgagaacc aagcgcctcg gctacaccga tcttcagctc tccatcatcg
     4861 gtttgggctc tttcgccatg ggcggcggcg ggtggaaatt cagctggggg ccccaggagg
     4921 acgaggactc gatacgcgcc atcgtgcgag gggtggagtt gggtgtcaac tggatcgaca
     4981 cggccccggt ttacggcctc ggccattccg aggaggtagt gggagcggcg ctcaagcggt
     5041 gtagccggaa accgctggtt gccaccaagt gtgggcgcag gtgggacggt gacgggaaca
     5101 tcttgcgccg gctgcggaag gccagcgtgc gggaggaagc cgaggccagc ttgcggcgcc
     5161 tcggggttga ggcgatcgat ctgtatcaga tccactggcc cgagcccgaa gaggagttcg
     5221 aagagggctg ggatgccatg gcgcagctcg tcaaagaggg gaaggtccgg tacctcgggg
     5281 tctccaactt cagcgtggcg cagatgagac gggtgcaggc catccatccc atcgcctccc
     5341 tgcagccgcc gtacagcctg ttacgccggg atatcgaatc cgagatcctg ccgttctgtt
     5401 cccagcatca tatcggggtg attccctata gtcccatgca gaaggggctg ctgactggca
     5461 cgttctcggt ggagcgggcg aagaatcttc cgccggacga ccaccgccgg aacgacccca
     5521 t
//
LOCUS       DBNGZC010000765         2460 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1829027, whole genome shotgun sequence.
ACCESSION   DBNGZC010000765 DBNGZC010000000
VERSION     DBNGZC010000765.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1829027"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="AC16G0_13145"
     CDS             <1..861
                     /locus_tag="AC16G0_13145"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR5100597.1"
                     /translation="IVSLGMVGIAASLIGGAPFLKAAAAGEAPPSLPEVHAHRGGAGL
                     APENTLAAFRKALDLDVDVLEMDMQITRDGEIVIIHDDTLDRTTDGRGRVTDFTLTEL
                     KQRDAGRHFGSQFTGERIPTLREVIALVQSKGNARVRLNIETKFAKGREGKPADFEEK
                     VLAILRESGFVERTILQSFYHPSLAKMKTLEPRIKLALLMGGNQASRDPVELVRQYQA
                     DYYSCDLRQVTSGLVSALHTAGLPVVPWTVNEEADVRRLLDTGVGKLAGDGIITNYPD
                     RVLNLLKVRR"
     gene            911..2257
                     /gene="ugpB"
                     /locus_tag="AC16G0_13150"
     CDS             911..2257
                     /gene="ugpB"
                     /locus_tag="AC16G0_13150"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019459816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HIR5100598.1"
                     /translation="MRVSRRTLIGLIAGLTLFGAWAAAPAEAQQRVQVEFWHGLSQPL
                     GGILEKVVADFNASQNKYQVNATFKGQYPETMMAAIAAFRSGNAPHIVQMFEVGTATM
                     MAAGRAIKPVYELAKETGVNIDPKNYVGGVRAYYSTPDGKLVSMPFNSSTTVTWYNKD
                     AFKKAGLDPEKPPRTWEETFAAARKIVAAKAAPCGYTMAWPTWTQYENFSAIHNVPLA
                     TKANGMKGMDAELKINSPLHVKHLQALMDLQKEGVFKYGGRDGAADALFPSGECAIFH
                     GSSGMRARVIREAKFQWGETFLPYYKGTPGAPKNSIIGGASFWVMASPKRTSEDYRAV
                     AEFFNYISRPEVDAKWHMDTGYVPITLGGFALASGQGYYIKNPGADIPFRQLTLTEPT
                     ENSMGLRLGNMPEIRVVIQEEMEKAFQGQQTAKQALDNAVKRGNVILRNFERTNRQ"
     gene            2342..>2460
                     /locus_tag="AC16G0_13155"
     CDS             2342..>2460
                     /locus_tag="AC16G0_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596409.1"
                     /note="with UgpEC is involved in the uptake of
                     glycerol-3-phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter permease"
                     /protein_id="HIR5100599.1"
                     /translation="MRRRVIFPNKLLPYLLVAPQLLITIVFFFWPAAQAIYYSV"
BASE COUNT          492 a          756 c          785 g          427 t
ORIGIN      
        1 atcgtcagtc tggggatggt gggtatcgcc gcgtccctta tcggcggtgc gcctttcctg
       61 aaggctgccg ccgccggaga ggcaccgccg agtctccccg aggtgcacgc ccaccgagga
      121 ggggctggcc tggcgcctga aaataccctg gcggcgttcc ggaaggctct ggacctggat
      181 gtggacgtgc tggagatgga catgcagatc acccgggacg gggagatcgt catcatccac
      241 gatgacaccc tggaccgcac caccgatggg cggggccgcg tgacggactt cacgctgacc
      301 gaactcaagc agcgggacgc gggacgccac ttcggatccc agttcactgg ggagcgcatc
      361 ccgaccctgc gcgaagtcat tgccctggtt cagtccaagg ggaacgcccg cgtacgcttg
      421 aacatcgaaa ccaagttcgc caaggggcgg gagggcaagc ccgcggattt tgaagagaag
      481 gttctggcca tcctccgcga gagcggcttt gtggagcgga cgatcctgca atccttttac
      541 cacccttcgc tggcgaagat gaagacgttg gagccacgca tcaagctggc cctgttgatg
      601 ggcggcaacc aggcatcgcg cgacccggtg gagctggtcc gacagtatca ggccgactac
      661 tactcctgtg accttcgaca ggtgacgagc ggtctggtgt cggcgctcca cacagcgggg
      721 ctcccggtgg tgccatggac tgtgaacgag gaagccgatg tgcgacgcct gctggacacc
      781 ggcgtgggga aattggcggg cgacggaatc atcacgaact atccggaccg tgtcctgaac
      841 ctcttaaagg ttcgtcggtg aacttcttga gagtggcaga tgcggcacct gtcgaaagga
      901 ggaggcagcc atgagagttt ctcgtagaac gctgatcggt ttaatcgccg gtctcacgct
      961 cttcggcgcc tgggccgccg cgccagccga agcccagcag cgcgtccagg tggaattctg
     1021 gcatggcctc agccagcccc tgggcggcat cctggagaag gtcgtggccg acttcaacgc
     1081 atcgcagaac aagtaccagg tgaacgccac gttcaaaggc cagtatcctg aaaccatgat
     1141 ggctgccatc gccgcgtttc gctcgggcaa tgcgcctcat atcgtacaga tgttcgaggt
     1201 ggggaccgcc accatgatgg cggctggcag ggccatcaag ccggtgtacg agctggccaa
     1261 ggagacgggg gtcaacatcg atcccaagaa ctacgtcggc ggcgtgcgcg cgtattacag
     1321 cacgccggac ggcaagctgg tctccatgcc attcaacagc tcgacaacag tcacgtggta
     1381 caacaaggac gccttcaaga aggcgggatt ggaccccgag aagccgcctc ggacctggga
     1441 ggagacgttt gccgcggcgc gtaagatcgt ggcggccaaa gcggctccct gcggctacac
     1501 catggcctgg cccacctgga ctcagtacga aaacttcagc gccatacaca acgtgcctct
     1561 cgccacgaag gcgaacggca tgaagggcat ggacgccgaa ctgaagatca acagcccgct
     1621 gcacgtgaag cacctgcagg ccctgatgga tctgcagaag gaaggggtgt tcaagtacgg
     1681 cgggcgagac ggggccgccg atgcgctgtt cccgtcgggc gagtgcgcca tcttccatgg
     1741 ctcatccggc atgcgcgccc gcgtcatccg ggaggcgaag ttccagtggg gcgagacctt
     1801 cctgccgtac tacaagggca caccgggggc tcctaagaac tccatcatcg gaggcgccag
     1861 cttctgggtg atggcctcgc ccaagcgcac gtcggaggac tacagggctg tggcggagtt
     1921 cttcaattac atcagccggc cggaggtgga cgccaagtgg cacatggata ccgggtatgt
     1981 gcccatcacg ctcggcggat tcgcgctggc gtccgggcag ggttattaca tcaagaatcc
     2041 gggggccgat attccgttcc gccaactcac gctcaccgag cccacggaga actccatggg
     2101 tctacggctg ggcaacatgc cggagattcg tgtcgtcatt caagaagaga tggagaaggc
     2161 cttccaaggc cagcagaccg ccaagcaggc cctggacaac gccgtgaagc gcggaaatgt
     2221 catccttcgg aacttcgagc ggaccaaccg ccagtaggct tcccccaccc cagccctctc
     2281 cccacctccg gggagagggt gaggggggag actggagctt tcccatgtcc gggagctgct
     2341 gatgcgccgg cgcgtgattt ttcccaacaa acttctcccg tacctgctcg tcgcccctca
     2401 gctcctcatc acgatcgtct tctttttttg gccggccgcc caggcgatct actattcggt
//
LOCUS       DBNGZC010000766         1604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_363492, whole genome shotgun sequence.
ACCESSION   DBNGZC010000766 DBNGZC010000000
VERSION     DBNGZC010000766.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_363492"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..266
                     /locus_tag="AC16G0_13160"
     CDS             93..266
                     /locus_tag="AC16G0_13160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100600.1"
                     /translation="MGTPAFGTAVTGEKPEDIQRGLAVWDGVVDEVIVRAVVARDAIE
                     EVLDLVEAARPSA"
     gene            396..1439
                     /locus_tag="AC16G0_13165"
     CDS             396..1439
                     /locus_tag="AC16G0_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HIR5100601.1"
                     /translation="MPSIDASRLTRICHNILLKMGASEEEATTVAQHQIGANLAGHDS
                     HGIILLPTYVDRIQKGHIVPGARFEVVHETPTTARINGNWGFGQVVQTRATRLAIEKA
                     RKNMLAAVTVAYQSHVGRLGDYPLMAAAEGMIGMLVCDSGRGPKYVVPFGGRDARLGT
                     NPISMALPSNLEAPIFVDMATSQVAGGKILVHRNRRQPVPLGWLVDKNGNPTTDPNDY
                     YAGGALLPLGGLDAGHKGYGLSTMVDVLAGVLTGLGWGIDPAGKHNDGSLFIAINVAA
                     FRPLEDFKAEVTDFAKFLKASRPAQGFEAVLYPGEIEWQRTQERLRTGIPIEDETWTE
                     IQKLAASVSVSTE"
BASE COUNT          322 a          487 c          529 g          266 t
ORIGIN      
        1 gcgcggcgcg cggcggcgag gcgcttgctc gtctccagcg tgaggcggca ggctacgagg
       61 cgatcccgca ctagagcgca catttcgagc gcatggggac ccccgccttc gggaccgccg
      121 ttaccgggga aaagccggag gacattcagc gcggcctagc tgtctgggat ggggtggtgg
      181 atgaggtcat cgtgcgagca gtcgtcgcgc gcgacgcgat cgaggaggtc ctggatcttg
      241 tcgaggcagc ccggccaagc gcatgacgcc gaacctcgcc gctcctgcta cgttctcacc
      301 ggaagaggtg gggtcacgca gcgaagccat gcaatttcat ccgcggcgac acccagcctg
      361 tgttcgacgg gaaatcctct gagaaaggac agcacatgcc gagtatagat gcttcgcgct
      421 tgacgcgtat ctgtcacaac atcctcctca agatgggtgc cagcgaggaa gaggcaacta
      481 cggtggcaca gcatcagatc ggtgccaatc tggcgggcca tgactcgcat ggcatcatct
      541 tgctccccac atatgtcgac cgaatccaga agggtcacat cgttcccggc gccagattcg
      601 aggtggtgca cgagacgccg accacggccc gcatcaacgg gaactggggc tttgggcagg
      661 ttgtacagac cagggccacc cgcctggcga tcgagaaggc caggaagaac atgctggcgg
      721 cggtgaccgt cgcctaccag agtcatgtgg gccgactggg cgactatccg ctgatggccg
      781 cggcggaagg gatgatcggc atgctcgttt gcgactcagg ccgcgggccg aaatatgtgg
      841 tgcccttcgg gggccgcgat gcccggctcg ggacgaaccc gatctccatg gcgctgccca
      901 gcaacttgga ggcgcccatc tttgtggata tggcgacgag ccaggtggcg gggggcaaga
      961 tcctcgtgca ccgaaatcgc cgccagccgg tccccttggg ctggttggtc gacaagaacg
     1021 gaaacccgac caccgacccc aatgactact atgcgggcgg ggccctgctg ccgttgggcg
     1081 gactggatgc cgggcacaaa gggtatggct tgagcacgat ggtggacgtc ctggccggag
     1141 tcctcactgg cctgggttgg gggatcgacc cggccggcaa acacaacgac ggttctctgt
     1201 tcatcgccat aaacgtggcg gcatttcgcc cgctggagga tttcaaggcc gaagtcacgg
     1261 attttgccaa gttcctgaag gccagccgtc cagcccaggg attcgaggca gtcctgtatc
     1321 cgggtgagat cgagtggcaa aggacgcagg agcggctgcg cacaggaatc cccattgagg
     1381 acgaaacctg gaccgaaatc caaaagctgg cagcctccgt gagcgtgtcg acggagtagg
     1441 tcctctggtc ccccactcga atgactgacg acgccgaccg gagtcaacga catcccggcg
     1501 tttgtgacgg gagagcgatc ccgaacccca accatccaat ctcggaggtg cccatgcttt
     1561 cagccgagat ctccagcctc ctcgaacaga caacgacgag cgcg
//
LOCUS       DBNGZC010000767         1873 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2122190, whole genome shotgun sequence.
ACCESSION   DBNGZC010000767 DBNGZC010000000
VERSION     DBNGZC010000767.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2122190"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="AC16G0_13170"
     CDS             <1..710
                     /locus_tag="AC16G0_13170"
                     /inference="COORDINATES: protein motif:HMM:NF014065.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - L-glutamate N-acetyltransferase
                     activity, acting on acetyl-L-ornithine as donor [Evidence
                     IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional ornithine
                     acetyltransferase/N-acetylglutamate synthase"
                     /protein_id="HIR5100602.1"
                     /translation="QRVAIGGMAKGSGMIAPHMATMLAVIASDAKVAAPALQGALRRA
                     VDQSFNCITVDGDTSTNDTVLCFANGAAGAPSLSPGTAAFRTFQAGLEMVCRRLAVQV
                     ARDGEGATKLVEVRVMGARTPREARLAAETVANSPLVKTTLFGEDINWGRIMMALGRS
                     GAGFDPEMVDLFVDEIPVVRKGVGLGRLAEDAANERMRRREFALVADLHAGRGQRRIW
                     TTDLSQDYVRINAGYRS"
     gene            1068..>1873
                     /gene="rpsB"
                     /locus_tag="AC16G0_13175"
     CDS             1068..>1873
                     /gene="rpsB"
                     /locus_tag="AC16G0_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR5100603.1"
                     /translation="MSAVTIKELLEAGVHFGHQTKRWNPKMKRYLFGERNGIYIIDLQ
                     KTLKKFQEAQSFVREVAARGDSVLFVGTKKQAVDAVQEEAGRSGMYFVNRRWLGGTLT
                     NFQTITKSISRLKQLEDMKATGKFEAFSKKEVLKLEREMQKLNNALLGIKGMEQIPGA
                     VFIIDPKKERIAVRESRRLAIPVVAVVDTNCDPEEIDYPIPGNDDAIRAIRLITSRLA
                     DAIVEGRGEAAKAGLTVEEEMEKAAAGPTAVAGEAAGAPSASPVASVAGAA"
BASE COUNT          356 a          548 c          675 g          294 t
ORIGIN      
        1 agcagcgtgt ggccatcggg ggcatggcca aggggtcggg catgatcgcg ccgcacatgg
       61 cgaccatgct ggcggtcatc gcctccgacg ccaaggtggc cgcgccggcg ctacaggggg
      121 ccttgaggcg cgcggtggat cagagcttca actgcatcac cgtggacggc gacaccagca
      181 ccaatgatac ggttctgtgc tttgcgaacg gggcggcggg ggcgcccagc ctctctcccg
      241 gcacggcggc cttccgaaca ttccaggcgg gtttggagat ggtgtgccgc cgtctggccg
      301 tccaggtcgc gcgggatggt gagggggcga ccaagctggt cgaggtccgg gtcatgggtg
      361 cccgaacacc cagggaggct aggctggcgg cggagacggt ggcaaactcc ccgctcgtca
      421 agacgacgct cttcggcgag gacattaact ggggacggat catgatggcc ctgggccggt
      481 cgggcgcggg gtttgatccc gagatggtgg atcttttcgt ggatgagatc cccgtggtcc
      541 ggaagggcgt cggcctcggg cgccttgccg aagacgcggc caacgagcgg atgcgccggc
      601 gggaattcgc cctggtggct gacctccacg ccgggcgcgg gcagaggcgc atctggacca
      661 ccgacctctc ccaggactac gtccggatca acgccggcta ccggagctga actacagctt
      721 gatccgcaga tgacgcagat ttcgtagatt tcaggaatcc ccgggaaaga acaaggatca
      781 ttctcgcgag agcgtggtct gcagggaacg cggaacggat ctcgtccggt acctctgaat
      841 ctgcgtagtc tgcgaaatct gcggtcaatt ttgcggtggt tgttcgactc ggcacggggg
      901 aaagacctgg ccggtgagag gggcgtgtgg tatacgaaac gacggcccgt cgtcgtggcg
      961 ggcccatttc acacgcacgc ggatccccgc ggaggtgccc cccggaaacc gggggtcccg
     1021 cggggaagtg aggcgtgcgg cggaccaacc gatatgggag gtgactcgtg agtgcggtga
     1081 ccatcaagga attgttggag gcaggggtcc acttcggaca ccagaccaag cgctggaatc
     1141 cgaagatgaa gaggtacctc ttcggtgagc ggaacggcat ctacatcatc gacctgcaga
     1201 agaccctgaa gaagtttcag gaggcccaat cctttgtgcg ggaggtggcc gcccgggggg
     1261 actcggtcct gttcgtgggg acgaagaagc aggccgtgga tgcggtccag gaggaggccg
     1321 ggcgctccgg gatgtacttc gtcaaccggc ggtggctggg cgggaccctg acgaacttcc
     1381 agaccatcac caagagcatt tcgcgcctga agcaactcga ggacatgaag gccacgggga
     1441 agttcgaggc cttctcgaaa aaagaggtcc tcaagctgga gcgggagatg caaaagctca
     1501 acaacgccct gctggggatc aagggcatgg agcagatccc gggcgcggtg ttcatcatcg
     1561 accccaagaa agagcggatc gcggtgcgcg agtcccggag actggccatc cccgtggtgg
     1621 cggtcgtgga taccaactgc gacccggagg agatcgacta ccccatcccg gggaacgatg
     1681 acgccatccg cgccattcgc ctgatcacat cacgcttggc ggatgccatc gtcgaagggc
     1741 gcggtgaggc ggccaaggca ggcctcaccg tcgaggaaga aatggagaag gctgccgcag
     1801 gccccaccgc cgtcgcaggc gaggccgcgg gtgcccccag cgcctccccc gtggcctcgg
     1861 tggccggggc ggc
//
LOCUS       DBNGZC010000768         5479 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_653297, whole genome shotgun sequence.
ACCESSION   DBNGZC010000768 DBNGZC010000000
VERSION     DBNGZC010000768.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5479)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_653297"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="AC16G0_13180"
     CDS             complement(<1..470)
                     /locus_tag="AC16G0_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR5100604.1"
                     /translation="MDLFGNLAMGFGVALTPLNLLYAFVGALLGTLIGVLPGIGPVPT
                     IAMLLPITYYLPPVSALIMLAGIYYGASYGGSTTAILVNIPGEISSVITVLDGHQMAR
                     QGRAGAALCISALGSFFAGCVATVVIAAFAPPLIEVALKVDAAEYFSLIVLGLLS"
     gene            complement(470..934)
                     /locus_tag="AC16G0_13185"
     CDS             complement(470..934)
                     /locus_tag="AC16G0_13185"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR5100605.1"
                     /translation="MSLVRNLKDFYAGLLFVAIGLVDLVIVRSYPLGTASRMGPGYFP
                     RILGILLVVLGALLSLRGFRSGGEPMSRWHWRPLTIVLLSVLIWGLTAQGLGLVVASL
                     ALVFVSSVASKEFGWREALLSGAIQAVAMAALFIYGLGIPLPVWPVFLGGGR"
     gene            complement(940..1899)
                     /locus_tag="AC16G0_13190"
     CDS             complement(940..1899)
                     /locus_tag="AC16G0_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013743337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HIR5100606.1"
                     /translation="MFKHAWIGVIILAFAGLAQAQTYPNKPIRMIVPWPPGGGVDTTA
                     RMISPPLGERLGQPIAIENRGGAGGNIGTEVAAHEKPDGYTLLMASLSPHSVNPYLYS
                     RLGFDPVKSFTPIALLWTVPNILVVPAASPFKTAKELIEYAKANPGKLNFGSGGIGSS
                     QHLSGVLLGTMAKIDIVHVAFKGAGPSQTALVAGHLDLMLDTPPALVQVAAGKLRALA
                     VTSRTRNPAIPDVPTFDEVGVPGVYVEVWYGIAGPAGIPREIVDRVNKETNAVLQTPE
                     MKKRALEYAAQIRPGTPEAFEQFMISELKRYEAVVKASGAKLE"
     gene            complement(2166..5036)
                     /locus_tag="AC16G0_13195"
     CDS             complement(2166..5036)
                     /locus_tag="AC16G0_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HIR5100607.1"
                     /translation="MPTASAAPDVSLTPSALQELLGRIIEPKRVLTRPIDRIAYAADA
                     SFYRLIPRAVVQSRSVDEIQALFGFSHTHRVPLTFRAGGTSLSGQAITDGILVEVARH
                     WRQVKVEEGGKRVRVQPGVIGGHVNQALKAFRAKMGPDPASINACMMGGILANNSSGM
                     CCGVTQNAYHTLHSLTFVLPSGTVIDTALPQADEILRQKEPTLAQGLLELRRKILASP
                     RLAERVRAKYKMKNTTGYSLNAFLDYERPVEIMRNLLIGSEGTLAFIAEAVLNTVSDL
                     PVKYAGLLLFPDVHAACAAIGPLRDADAKAVELMDRASLRSVQEQPGIPPNIKGLPQD
                     AAGLLAEFQATDERDRSTLEKRASEAVGGLRLLEPGRFTHEAAEQALLWKIRQGLYPS
                     VGAVRKQRTTVIIEDVVFPIERLADATVELIHTVGRHGYDEAIVFGHAKDGNLHLVIT
                     QSYSSQAEVDRYARFMDDMVDLVVKRHDGALKGEHGTGRNIAPFVETEWGPEGLAIMR
                     RLKSLVDPNGLLNPGVIINADPKAHLADLKPMPVVEDTVDKCIECGYCESKCPSLDLT
                     LTPRQRIVVRREMARLAESGTNRDLLAALDAEFPYMALDTCAVDGLCATACPVGIDTG
                     QLTKHFRRLRHPAWAHGVARRLAVNFARLEPTMRLGLRMGHVVQSLFGAGAMTGLTRV
                     IRAVVRQPFPLWSADMPRPARGRPATVQANAQAVYFPSCISRVMGHLPGEPHEISLME
                     AFVAVAGRAGMPVFIPQDVEGTCCGVPFSSKGYNQGHAVAMNRAIERFWAWSAGGRLP
                     IVVDTSPCTYGLLNGRDSLTPENQQRFDGLRILDGTAFVHDQLLPRLTIRRRVRSVVL
                     HPVCSVIKLNLSPKLEGIARACSEQVVVPLAAGCCGFAGDRGFLFPELTGSATRREAA
                     EVQAGGHDGYFSSSRTCEIGMSRATGQIYRSYLYLLEQATRP"
     gene            complement(5098..>5479)
                     /locus_tag="AC16G0_13200"
     CDS             complement(5098..>5479)
                     /locus_tag="AC16G0_13200"
                     /inference="COORDINATES: protein motif:HMM:NF014628.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LutC/YkgG family protein"
                     /protein_id="HIR5100608.1"
                     /translation="LVAWDVAGSLKGLGVEIVSPQADKHLVAQCELGVTGVDAALSET
                     GTLLLRSSPERPRVVSLLPRVHLAILTRAALRADLHQAFTEVKHDPYCVLVTGPSRTS
                     DIELTLTLGVHGPKSLYAWVMDAQ"
BASE COUNT         1028 a         1774 c         1777 g          900 t
ORIGIN      
        1 gagagcaggc cgagcacaat cagtgaaaaa tactccgcag cgtcgacctt gagcgccacc
       61 tcgatcaggg gcggcgcaaa ggcggcgatc accaccgtcg ccacacaccc ggcaaagaag
      121 gaccccagcg cggaaatgca cagggccgcc ccggcccgcc cctgccgagc catctggtga
      181 ccgtcgagga cggtgatgac cgaggagatc tcgccgggga tattgaccag gatggcggtc
      241 gtcgagccgc cgtaggatgc gccgtagtag ataccggcga gcatgatcag cgctgagact
      301 ggcggcagat aataggtgat ggggagcagc atggcgatcg tcggcaccgg gccaatgccg
      361 gggagaaccc cgatgagcgt gcccagcaat gcgccgacaa aagcatagag caggttgagc
      421 ggcgtcaagg ccacgccgaa tcccatggcg aggttgccga acaagtccat cagcggcctc
      481 ccccgaggaa gaccggccag accggcaatg ggatccccag accgtaaata aacagcgcgg
      541 ccattgccac cgcctggatg gcgccgctga ggagcgcctc cctccacccg aattctttac
      601 ttgccaccga agacacgaaa accaacgcca ggctggcaac caccagcccc agcccctgcg
      661 cggtcaggcc ccagatcagc acactcagca ggacaatggt cagcggtcgc cagtgccagc
      721 gggacatcgg ctcgcccccg gagcgaaaac cgcgcaggct gagaagggcc ccaagcacca
      781 cgagcaggat gccgaggatg cggggaaagt agccggggcc catgcgggag gcggttccga
      841 gggggtagga tcgaacgatc acgaggtcca ccagaccgat cgccacgaac agcaatccgg
      901 catagaagtc cttgagattg cgcaccagcg acatgccggc tactccagct tggccccgga
      961 ggccttcacg acggcctcgt agcgcttcag ttcggagatc atgaactgct caaatgcctc
     1021 cggcgtgccg gggcggatct gtgccgcgta ctccaatgcg cgcttcttca tttccggcgt
     1081 ttgcagaacc gcattggtct ccttgttcac ccgatcgacg atctccctcg ggatccctgc
     1141 cgggccggca atgccgtacc agacttccac gtacacgccg gggacgccga cctcgtcgaa
     1201 tgtcggcaca tccgggatcg ccgggttccg cgtcctggag gtcaccgcca gcgcccgaag
     1261 ttttccggcc gcgacctgca ccaaggccgg cggggtatcg agcatcaggt ccagatggcc
     1321 tgcaaccagt gccgtctggg aggggccggc tcccttgaat gccacatgca cgatgtcgat
     1381 cttcgccatg gtgcccagca ggacgcccga caggtgctgg gaggacccga tgccgcccga
     1441 cccgaagttg agcttgccgg gattggcctt cgcatactcg atcaactcct tcgccgtctt
     1501 gaagggtgag gcggccggca ccaccaggat attcggcacg gtccatagga gcgcgatcgg
     1561 cgtgaagctc ttgaccgggt cgaagccgag ccgcgagtaa aggtaggggt tgacggaatg
     1621 cgggctgagg gacgccatga gcagggtgta gccatccggc ttctcgtgcg cggccacctc
     1681 ggtgccgatg ttgccccccg cccctcctct attttcgatg gcaatgggct gtccgagtcg
     1741 ttcccccagc ggcggcgaga tcatccgcgc cgtggtgtcc acgccgcccc ctggaggcca
     1801 gggcacgatc atcctgatcg gtttgtttgg gtacgtctgc gcctgggcga gtcctgcgaa
     1861 ggccaaaatg atcacgccaa tccacgcatg cttgaacatg agtttcctcc ctgagcatct
     1921 gcggttcgct tcagccgaga ttatcccttt ctctcagcgt gtgagccgat gcctgcgaaa
     1981 cgcagagggc accggaaaat gaatgggtca cggatattat gaaaccatga tgctgtcaac
     2041 actattcccg gcggctattc ccaaaagtat ctgagtacgt aacatcggaa gagccgaagc
     2101 gcatccaaga gttgtttacg gggtatcggc ggaaccgacg acggaatgag aaggtcgtga
     2161 ggtaattacg gcctcgtcgc ctgctccagc aggtagaggt aggagcggta gatctggccc
     2221 gtggcgcggc tcatgccgat ctcgcaggtc cggctgctcg aaaagtagcc gtcgtgcccg
     2281 cccgcctgca cctcggcggc ctcgcgccgc gtggcggacc cggtcagctc ggggaagagg
     2341 aacccccggt cgccggcaaa accgcagcag cccgcggcaa gcggcaccac aacctgttcg
     2401 ctgcacgccc gcgcgatccc ttccagcttc ggcgagaggt tcagcttgat gaccgagcag
     2461 acggggtgga gcaccaccga ccggaccctc cggcggatgg tcagtcgggg cagaagctgg
     2521 tcgtggacga aagccgtgcc atccaggatg cgcaggccgt cgaaacgctg ctggttctcg
     2581 ggcgtgagag agtcccggcc attcaggagg ccgtaggtgc aggggctggt gtccaccacg
     2641 atcggcagtc ggccgcccgc cgaccatgcc cagaagcgct cgatcgcccg attcatggcg
     2701 acagcgtgcc cctggttgta acccttggac gaaaacggca cgccgcagca ggtcccctca
     2761 acgtcctgcg ggatgaagac cggcatcccc gctcgccccg ccacagccac aaaggcctcc
     2821 atcaggctga tctcatgcgg ctctcccgga agatggccca tgacgcgcga gatgcaactc
     2881 ggaaagtaaa cggcttgggc gttcgcctgc accgtggcag gccgtccccg ggcggggcgc
     2941 ggcatgtctg cgctccacaa cgggaaaggt tgccggacga ctgctcgaat cactcgggtg
     3001 agaccggtca tggccccggc gccgaagagc gattggacga cgtggcccat ccggagcccg
     3061 agacgcatcg tcggttccag gcgggcgaag ttgacggcga gcctgcgggc caccccatgg
     3121 gcccaggcgg gatggcgtag gcggcggaag tgtttggtga gttggccggt gtcgatcccc
     3181 accggacagg ccgtggcgca caggccgtcc acggcgcagg tgtccagcgc catgtacggg
     3241 aattcggcat ccagggctgc caagaggtcc cggttggtcc cgctctcggc gaggcgggcc
     3301 atctcgcgcc gcaccacgat gcgctggcgt ggggtgagcg tgagatcgag gctcggacac
     3361 ttcgactcgc agtagccgca ctcgatgcac ttgtccacag tatcttcgac gaccggcatg
     3421 ggcttgaggt cggcgaggtg ggccttggga tcagcgttga tgatgacgcc agggttcagc
     3481 agcccgttcg gatccaccag cgactttaag cgccgcatga tggccaagcc ttctggcccc
     3541 cactcggtct ccacgaaggg ggcgatgttc ctacccgttc cgtgctcccc cttgagggcg
     3601 ccgtcatgcc gcttcaccac caggtccacc atgtcgtcca tgaagcgtgc gtaccggtcc
     3661 acctcggcct gcgaactgta cgactgggtg atcaccaaat gcagatttcc gtccttggcg
     3721 tgaccaaaga cgatcgcctc gtcgtacccg tgtcggccga cggtgtggat cagctcgacg
     3781 gttgcatccg ccaacctctc gatcgggaag accacgtcct cgatgatcac cgtggtcctt
     3841 tgctttcgca cggcccccac agacgggtac agcccctggc ggatcttcca caacagagcc
     3901 tgctccgcgg cctcgtgggt gaagcggccg ggctcaagca gccgaagacc tcccaccgct
     3961 tcactggcac gcttctccag cgtagaccga tccctctcat cggtagcctg aaactccgcc
     4021 aggagcccgg cggcgtcctg ggggaggcct ttgatattcg gggggatccc aggttgctcc
     4081 tgcaccgacc ggagcgaggc gcgatccatc agttccaccg ctttggcgtc cgcatcgcgc
     4141 aacggcccga tggccgcaca cgccgcatgg acgtccggga acagcagcag ccccgcgtac
     4201 ttcacgggga ggtccgacac cgtattgagc acggcctcag cgatgaaggc cagggttccc
     4261 tccgagccga tcagcaggtt ccgcatgatc tccaccggcc gttcgtagtc gagaaaagca
     4321 ttcagcgaat agccggtggt gttcttcatt ttgtacttgg ccctgactcg ctcggccagc
     4381 cgcggactgg ccaggatctt gcggcgcagc tccaggagac cctgggcgag tgtgggctct
     4441 ttctggcgga gaatttcatc cgcctgcgga agggcggtgt cgatcacagt ccccgaggga
     4501 agcacgaagg tcagcgagtg gagcgtgtgg taggcgttct gggtaacccc gcagcacatt
     4561 ccgctggagt tattggccag gatcccgccc atcatgcagg cgttgatgga agccgggtcc
     4621 ggtcccatct tggcccgaaa tgccttgagt gcctggttca cgtggccgcc gatcaccccg
     4681 ggctggacgc ggaccctttt cccgccctcc tccactttca cttggcgcca gtgacgggcc
     4741 acctccacca agatcccgtc ggtgatcgct tggcccgaga ggctcgtacc cccggcgcgg
     4801 aacgtcaggg gaacgcgatg cgtatgactg aagccgaaga gggcctgaat ctcgtccaca
     4861 ctgcggctct gcaccaccgc ccgcgggatc aaccggtaga agctcgcgtc cgcagcgtag
     4921 gcgatgcggt cgatgggccg ggtgaggacg cgctttggct cgatgatcct tccgaggagt
     4981 tcctgcaggg cggagggggt cagggatacg tcaggagccg cagaggcggt cggcatgggc
     5041 agacctcctg gtagagcaag agagagatgc gctggacttg ggggaaagcc ggctggccta
     5101 ctgcgcgtcc atcacccacg cgtagagcga cttcggaccg tgcacgccga gggtgagggt
     5161 cagctcgata tcgctagtgc ggctggggcc ggtgaccagg acgcagtagg gatcgtgctt
     5221 cacctcggta aaggcctggt gaaggtcggc ccgcaaggcg gcccgggtca ggatcgccag
     5281 atggaccctc ggcaggagcg agacgacccg cggccgctct ggggaggagc gcagcaggag
     5341 cgtcccggtc tccgacagag ccgcgtccac acccgtcacc cctagttcac attgcgccac
     5401 cagatgtttg tccgcctgag gggaaacgat ctcgacgccg agacccttga gcgagcccgc
     5461 gacgtcccag gccacgaga
//
LOCUS       DBNGZC010000769         1953 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_555068, whole genome shotgun sequence.
ACCESSION   DBNGZC010000769 DBNGZC010000000
VERSION     DBNGZC010000769.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_555068"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="AC16G0_13205"
     CDS             <1..1079
                     /locus_tag="AC16G0_13205"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR5100609.1"
                     /translation="YFTGTVQDAWLVIAREGPPILLVRRSLERARAESALEHILPLED
                     PREIPRLLGRHGLGWLKRVGLEFDVLPLQQYVRLGRLFPTMQFVDASMLVREIRMLKS
                     AYEVGCIRRAAAMQDEMATRMRETLRAGMTELELAAEIEAEARRRGHQGIVRTRRFNM
                     ECYYGHLLAGESGAVPSWLDSATGGVGVSPAFGNGAGARRIQAHEPILLDYVGVCDGY
                     ISDQTRLFSLGSLPEDLLRGYEACLTIREDILANLRPGVPAGEVHARAIALAGAQGYG
                     ARFMNTGPAQVSFVGHGVGIELDEIPYLAKGSNLVIQAGMALAVEPKIVFPGVGIVGV
                     EDTVLVTDGEPDCLTFTPREIVVL"
     gene            complement(1142..>1953)
                     /locus_tag="AC16G0_13210"
     CDS             complement(1142..>1953)
                     /locus_tag="AC16G0_13210"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR5100610.1"
                     /translation="ELAGGWPLGSDIPFPDAQVMAEEVARLRYSLTPEEVERYRWLGA
                     KATAAVEKTLQEARPGEKESEVVGRLCHELWKDRIDPVGLLAAADDRIARYRHPIPTE
                     RRSEKLLMLSVNARKGGLIVCLTRFVHFGKASKALLDRYQANVFVDCTFMVHTRPGTL
                     ARDVFRKGLDAYRERGFAEEWKLHHQGGSIGYQNRDYRVSPDTQEVIQDNQAFCWNPS
                     LTGTKSEDTILATSRGPEMITGPKTYPTLAMEVGGFPFIRPAILEKVHEQP"
BASE COUNT          322 a          590 c          658 g          383 t
ORIGIN      
        1 tctacttcac gggcacggtg caggatgcct ggctggtgat cgctcgggag ggacccccga
       61 ttttgctcgt ccggcgaagc ctggagcgcg cccgggctga gtcggccctg gagcatatcc
      121 tgccactcga ggacccacgc gagattccga ggctgcttgg gcgtcatggc ctgggatggc
      181 tgaagcgggt cggcctggaa ttcgatgtcc tgccgctcca gcagtacgtg cgactgggtc
      241 ggctgtttcc gaccatgcag ttcgtggatg cctcgatgct cgtccgcgag attcgcatgc
      301 tcaagtccgc gtacgaggtg ggctgcatcc ggagagccgc ggccatgcaa gacgagatgg
      361 ccacacggat gcgggagact ctccgggcag gcatgacgga gctggagctg gcggcggaga
      421 tcgaggccga ggcccgccgt cgggggcacc aggggatcgt tcgtacgcgg cgattcaaca
      481 tggaatgcta ctacgggcac ctcttggccg gggagagcgg ggcggtgccc tcctggctgg
      541 attccgccac ggggggtgtt ggggtctcac ctgccttcgg aaacggggcg ggcgcgcggc
      601 ggatccaggc tcacgaaccg atcctgttgg attatgtggg ggtctgcgat gggtatatct
      661 ccgaccagac caggctcttc tccctgggga gtttgcctga agatctgctt agggggtacg
      721 aggcctgtct cacgatccgg gaagacatcc tggccaacct acgtccgggt gtacctgcag
      781 gcgaggtcca tgcccgcgca atcgccctcg cgggggcaca ggggtacggg gctcggttca
      841 tgaacacggg accagcccag gtcagcttcg tcgggcacgg ggtggggata gagttggacg
      901 agatcccgta cctcgcgaaa gggtcgaatc tggtgatcca ggctggtatg gcgctggccg
      961 tggagcccaa gattgtcttt ccgggcgtcg ggatcgtggg tgtggaggac accgtcctgg
     1021 ttacggacgg cgagccggac tgcctgacgt tcacaccgcg agagatcgtc gttctctgag
     1081 gagggtggcc gttggccacc agcatgtggc ccttacgcgt tccaggccca cctccgacgg
     1141 atcaaggctg ttcgtggact ttctccagga tcgccggtcg gatgaagggg aacccaccta
     1201 cctccatggc aagggtgggg taggtcttgg gtcccgtgat catctctgga ccccgagagg
     1261 tggcgagaat ggtgtcctct gatttcgtcc ccgtgaggct tgggttccag caaaacgcct
     1321 ggttgtcttg gatgacctcc tgcgtgtcgg ggctgacgcg gtaatcccgg ttctggtagc
     1381 cgatggaccc tccctggtga tggagcttcc actcttctgc gaagccccgc tcccgatacg
     1441 cgtccagccc cttccggaaa acgtctcgtg caagggtgcc ggggcgcgtg tggaccatga
     1501 aggtgcaatc cacgaacacg tttgcctggt acctatccag caaggctttt gaggccttgc
     1561 cgaaatggac aaaacgggtg aggcaaacga tcagtccgcc cttgcgcgca ttgacggaga
     1621 gcatcagtag tttctcgctc cgacgctcgg tcgggatggg gtgacggtat cgcgcgatcc
     1681 gatcgtcggc agcagccagc agaccgaccg gatcgattcg atccttccac aattcgtgac
     1741 acagccgccc cacgacctct gattccttct cccccggtcg cgcctcctgc aaggttttct
     1801 cgacagccgc cgtggccttc gctcccaacc accggtatcg ctccacttct tccggtgtga
     1861 gggagtagcg gagccgggcg acctcctcgg ccatcacctg agcgtccggg aaggggatat
     1921 cgctacccag cggccaacct cccgcgagtt ccc
//
LOCUS       DBNGZC010000770         1772 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_849726, whole genome shotgun sequence.
ACCESSION   DBNGZC010000770 DBNGZC010000000
VERSION     DBNGZC010000770.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_849726"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="AC16G0_13215"
     CDS             complement(<1..559)
                     /locus_tag="AC16G0_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100611.1"
                     /translation="MADRINTAGAPAWQRGFWCLIAAQFQGAFNENGLKNLVVFLILG
                     MGMHAVDRDRLVLVVGALFSVPFLLFSMAGGYLADHYSKRTVTIWTRCFELGVMALAA
                     SGLAWGNLPLTLAAVFLASTQAALFGHSKYGLLPELLPPTRLSWGNGVIELGTFLAVI
                     AGSVCGAFFAEAFHGREAWSGAIFFG"
     gene            complement(1230..>1772)
                     /locus_tag="AC16G0_13220"
     CDS             complement(1230..>1772)
                     /locus_tag="AC16G0_13220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100612.1"
                     /translation="KPWRGRLALATQPSRRPAGSQSALTPGPLPKGVVSTLHRMTLAT
                     LLVLVAAPGLASATLDSFLSSVNVQARVDLPGFYAKVSAQFGVPVPQVEAVLGMVATP
                     ADAFMVFQLGQMAHRPPETVVQTYQAHKGKGWGVIAKELGIKPGSREFHALKNGNLVY
                     GDAATNGPGKGKGKVKGNKK"
BASE COUNT          344 a          556 c          582 g          290 t
ORIGIN      
        1 ggccgaaaaa gatcgcacca gaccacgcct cacgtccatg aaaggcctcg gcgaagaagg
       61 ctccacagac actgccggcg atcactgcca ggaacgtacc gagctcgatc acgccattgc
      121 cccaggacag ccgggtaggc ggcaggagct ccggcagcaa gccgtacttc gagtggccga
      181 atagcgcggc ctgtgtgctg gccagaaaga ccgccgccag agtgaggggc agatttcccc
      241 aggcaagccc gctcgcggcc aaggccatga cccccagctc gaagcaacgg gtccagatcg
      301 tcacagtgcg tttgctgtag tggtcggcga gatatccgcc cgccatggag aagagcagga
      361 acggcaccga gaacagcgcg cccacgacga gcacgagtcg gtcgcggtcg accgcatgca
      421 tgcccatccc gagaatgagg aagaccacca gattcttcag gccgttttca ttgaacgccc
      481 cttggaactg ggctgcgatg aggcaccaaa accctcgctg ccaggcggga gcgcctgcgg
      541 tgttgatccg gtctgccatc atccctccac ctcttcgcga cgccaagtaa cgtcgtgaac
      601 ctcccccacc acgatcctga tcgcctcgcg accggctaga ccctcccgct gcgtaccctg
      661 gacaagagca gtcgaggccc ggactgcgac gctgcctacg ggcgtcaaat cgtctcccct
      721 tctacaggag cgatgagagc atggaatgtg ccagcgcgcg gtggcatggg aggactcgag
      781 gccggatggg aagatcacgg aaatacaagg acttgcacct caccgggcgc gccttggagc
      841 gggaggaacg gatacaggtg tctaggggcg gatgcgggta tgcgctccct ctgctagctg
      901 gcctcgcctc tcaggtaacc cgaggactga caaggcttct tgggttacat ggggagccga
      961 atcctcgtgc gcagagtaga gagtaagggg atggtggcgc ctgcatcaag cgcgggaggg
     1021 agcaggagag ccgacacttg gaggccagga ctcagtcggg ggatcttgca tcctctgatc
     1081 tccacggaac tcttgctcaa gtggtggtcc gggggtacga aatcccaatt gtgcaagaca
     1141 tctcctccct gggggatcaa cccgcgccga acgggggcga acggcatccg gagagggtga
     1201 ctgaggagct ttggagtggg gtgaaaggat tacttcttgt ttcccttcac tttgcccttc
     1261 cccttgccgg ggccgttcgt cgcagcatcg ccgtacacga ggttcccgtt cttgagggcg
     1321 tggaactcac gggaacccgg cttgatcccg agttccttgg cgatgacccc ccaccctttg
     1381 cccttgtggg cctggtaggt ctggacgacg gtctcggggg gccggtgggc catctgcccc
     1441 aactggaaga ccatgaaggc gtccgcggga gtcgccacca tcccgagcac cgcctccacc
     1501 tgcggcaccg ggacgccgaa ctgggcgctc accttggcgt aaaacccggg caggtcaacc
     1561 cgcgcctgga cattgacgct gctcaggaag ctgtcgagag tggcagacgc gagcccgggc
     1621 gcggcgacga ggacgagcag cgtcgcaagc gtcatccggt gcaacgtgct gactactcct
     1681 ttcgggagcg gcccgggtgt gagagcgctc tggctcccgg cgggtcgcct ggacggctgc
     1741 gtggcgagcg cgaggcgtcc acgccagggc tt
//
LOCUS       DBNGZC010000771         3608 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2122375, whole genome shotgun sequence.
ACCESSION   DBNGZC010000771 DBNGZC010000000
VERSION     DBNGZC010000771.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2122375"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="AC16G0_13225"
     CDS             <1..762
                     /locus_tag="AC16G0_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011383711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR5100613.1"
                     /translation="IQQNVDPPYQFPNVQAVVHWLKDTPLRPSNIRAPGKVANSFATE
                     SFTDEVAALVHSDPLAFRQRHLTNPRGLEVLRRLAARMAWAPRPSPHQSDPKAALLTG
                     RGLAYVHYKHSENHVAMGMEVEVQRGTGRIRVTRVVCAHDCGLMINPDCVQSQLEGSI
                     LQSISRTLLEEVTFDRSRVTSLDWSSYPILTFPDAPVMEFDLIDRPDQPPMGVGEAAS
                     TPVPAALANAVFDATGVRLRSVPFRAERVKAALEG"
     gene            908..1636
                     /locus_tag="AC16G0_13230"
     CDS             908..1636
                     /locus_tag="AC16G0_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HIR5100614.1"
                     /translation="MRGARVGGNWSKILLATLSIAMAVAAVLGGAPPAVADDAMDAQQ
                     LVDRSRLALESFMADPNMEAFRDLLKRAKGVFIAPQVLKGAFVIGASGGSGVLLTRDG
                     RTGGWGGPAFYTMGGISFGLQAGGEASEVALLAMTERGVAAFQASSMKLGAEVGYAIG
                     PIGAGAAAASANLSADIISYVRSKGVFLGMSVDGAVVATRDALNTAYYGKDVTPSDIL
                     IRQSVRNPQANRLIELIANAAATK"
     gene            2016..2723
                     /gene="deoC"
                     /locus_tag="AC16G0_13235"
     CDS             2016..2723
                     /gene="deoC"
                     /locus_tag="AC16G0_13235"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014687629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR5100615.1"
                     /translation="MLTVDELAQMIDHSILLPGQTEKDLEEGIALARKYRVKCVVPKA
                     YQAARAKELLKGSNVRLCIAIAFPQGLNLPEVKRYEAEHALEQGAQELDMVINISALR
                     SHQYDFVEKDIQGVVEVARRFDAPVKVILETCLLTDEEKVAGCRIAEQAGAAFVKTST
                     ATQPGGATVEDIRLMRATVGPKVIVKASGYVTDIQKTLALYEAGARRFGTGYTANILE
                     GLRKLGGEAGAPHPRPA"
     gene            2846..>3608
                     /locus_tag="AC16G0_13240"
     CDS             2846..>3608
                     /locus_tag="AC16G0_13240"
                     /inference="COORDINATES: protein motif:HMM:NF037995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR5100616.1"
                     /translation="MRLRRRTCSAGRWSWILVGLCVLAWNLPGVAWGADTKVAKLGLI
                     APMGHPVQQASERMAKLVAERTQNRLQIQVFPGGTLGGEIDLRDGVSLGTVQMAGIGY
                     PILNGIVNEMEILNLYYLWRDRAHMMKVINGPIGAEFAQKLRGRNVEVLAANWQQGTR
                     QSLTKRPARNPKEFNGIKIRVTAGIPVYQDLWQAMGAITVPLPFPELYSALQQGVVDA
                     VELPLDWMYNGKFYRLGKNIDLTNHFIYSNCVLINT"
BASE COUNT          709 a         1080 c         1181 g          638 t
ORIGIN      
        1 atccaacaga acgtagatcc gccttaccag ttcccaaacg tccaggcagt ggtgcactgg
       61 ctgaaggaca cgccgctacg cccctccaac attcgggcgc cagggaaggt cgccaacagc
      121 ttcgccaccg aatcgtttac cgacgaggtc gcggcgctgg ttcattccga ccccctggcc
      181 ttccgccagc gtcacctcac gaatccgcgc gggctggagg tcttgcgccg cctggccgcg
      241 cgaatggcct gggcgccccg tccatcccct caccagtctg atccaaaggc ggcactcctg
      301 accgggcggg gcctggcata cgtgcactac aagcacagtg agaaccatgt ggccatgggg
      361 atggaggtgg aagttcagcg cgggaccggc aggatacgcg tgacgcgagt agtgtgcgct
      421 cacgattgcg gtctgatgat caacccggac tgcgtgcaga gtcagctcga aggcagcatc
      481 ctccagtcga tcagccgaac gctgctggag gaggtgacct tcgatcggtc gcgggtcaca
      541 agcctggact ggtccagcta tcccatcctc acattcccgg atgctcccgt catggaattc
      601 gatttgatcg accgtccgga tcagccgccg atgggtgtgg gcgaggcggc ctcgacgccg
      661 gtgcccgccg ccctagcgaa cgcggtgttt gacgcgaccg gggtgcgcct ccgctccgtt
      721 cctttccggg ctgaacgggt caaggcggcg ttggagggat gagtgggctg aatcccggac
      781 ggggtagaat gtcattcccg agccaagcgt ttcatgtggc tctgaagccc tgtcgaggat
      841 gcgagagctt ggcgcgagga gggcaaagag gcaggaaagg attgaacgtc agaacaggag
      901 gaaggtgatg cgaggagcaa gggtaggggg caattggtcg aagatccttc tggccacgct
      961 gagcatcgca atggccgtgg cggccgtcct cggcggtgcg ccgcccgctg tcgccgatga
     1021 cgccatggac gcccagcagc tcgttgaccg atctcggctg gcgcttgaaa gcttcatggc
     1081 cgatcccaac atggaggcat ttcgcgatct cctcaaacgg gccaagggcg tgtttatcgc
     1141 gccgcaggtt ctcaagggcg ccttcgtcat cggcgcctcg ggcggaagcg gggtcctgct
     1201 cacacgggat gggaggaccg gtggctgggg ggggcctgcc ttttacacca tgggagggat
     1261 aagctttggc ctgcaggcgg gcggtgaggc atctgaggtg gcgcttctgg ccatgaccga
     1321 gcggggagta gcggcgtttc aggcgagcag catgaagctc ggcgccgagg tcggatacgc
     1381 cataggaccg attggcgctg gcgccgcggc cgcgtccgcc aacttgagcg cggacatcat
     1441 cagctacgtc cgctccaagg gggtcttcct tggcatgtcg gtggatgggg cggtcgtggc
     1501 aactcgggat gccctgaaca cggcctacta cggcaaggat gtcacccctt cggatatttt
     1561 gatccgacaa tcggtcagga acccgcaggc caaccgcctg atcgagctga ttgcgaacgc
     1621 cgccgccacg aagtgactcc tgggtcaaag cctaccggta gcgcattgac tgaccgacag
     1681 gacaagcagc ccatacccct ggacatcctg acgccgtcgg tgctaggtct cttgcgcagc
     1741 gggaatccca acctggcaga cggccagtcg gccctgacct gaacctgggg tgcgcatgat
     1801 gaagtcctat gggtgccgcc tgcgagatgc ttcccgccct gcgaattcgt ttcgagagcg
     1861 cgttcggcct gcgcccaaga cctctcccat ttttctgaga cccatctgga gggaggcaac
     1921 ctcgacacag gttcgagcga catctgtctg tcgtgtgaga atccagccct gattgatgag
     1981 atgctgctgg cacaggtagg agaaaggaga atagcgtgct taccgttgac gagctggccc
     2041 agatgatcga ccactccatc ctgttgcctg gtcagaccga gaaggacttg gaagagggca
     2101 tcgccctcgc gcggaagtac cgcgtcaagt gcgtcgtgcc caaggcgtat caagcagccc
     2161 gcgccaagga gcttctgaaa ggatcgaacg tgcggctctg catcgccatc gcctttccgc
     2221 agggcctgaa cctgcccgag gtgaaacgct acgaggccga gcatgcgctg gagcagggcg
     2281 cccaagagtt ggacatggtg atcaacatca gcgccctcag gtctcaccag tatgactttg
     2341 tcgagaagga tatccagggg gtcgtggaag tggcgcgccg tttcgacgcc cccgtcaagg
     2401 tcatcctgga aacatgtctc ctgaccgacg aagaaaaggt cgccggctgc cggatcgcgg
     2461 agcaggccgg cgccgccttc gtcaagacct ccacagcgac ccagccgggc ggcgcgaccg
     2521 tggaggacat ccggttgatg cgggccaccg taggcccaaa ggtcatcgtc aaggcgtcgg
     2581 gctacgtgac agacatccag aaaaccctgg ccctctacga ggcgggtgcc cggcggttcg
     2641 gcacggggta taccgccaac atcttggaag gactccggaa gctagggggg gaagcaggcg
     2701 caccacaccc tcgacctgcc tgagaactgc cagccagacg atgggggcag tgagacccgt
     2761 ccggataaca tgcccaaccg aattgcgcca agttaggcag caactccttg cctgatcgga
     2821 caagggaacc cggaaggagg ccaagatgag actgcggcga agaacgtgtt ctgctggacg
     2881 ctggtcctgg atcctggtgg gtctctgcgt gcttgcctgg aacttacccg gtgtcgcctg
     2941 gggagcagat accaaggtgg ccaagctggg gctcatcgcg ccgatgggtc atcccgtcca
     3001 gcaggcctcc gagcgtatgg ccaaactcgt ggcggagcgg acgcagaatc gtctccagat
     3061 acaggtcttc ccaggtggaa cgctcggcgg tgagatcgat ctccgcgacg gcgtctccct
     3121 cgggaccgtt cagatggcgg gcatcgggta tcccatcttg aacgggatcg tgaacgagat
     3181 ggagattctg aatctgtact atctctggcg ggaccgggcg cacatgatga aggtcatcaa
     3241 cggacccatc ggggccgagt ttgcccagaa gctccgcgga cggaacgtcg aggtgctggc
     3301 ggcgaactgg cagcagggca cgcggcaatc gttgaccaag cgtcctgccc gcaacccgaa
     3361 ggagttcaac ggcatcaaga tccgggtgac ggccggcatc cctgtgtatc aggacctctg
     3421 gcaggcgatg ggcgccatca cggttccact cccgttcccc gagctgtact cggccctcca
     3481 gcagggggtg gtggacgcgg tggaacttcc cctggattgg atgtacaacg gcaagttcta
     3541 caggctgggg aagaacatcg atttgaccaa ccacttcatc tattcgaact gtgtgctgat
     3601 caatacag
//
LOCUS       DBNGZC010000772         1851 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_948964, whole genome shotgun sequence.
ACCESSION   DBNGZC010000772 DBNGZC010000000
VERSION     DBNGZC010000772.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_948964"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..418
                     /locus_tag="AC16G0_13245"
     CDS             278..418
                     /locus_tag="AC16G0_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100617.1"
                     /translation="MDECPAGLSGAHWRAAIRARRARRGIRKDDFERRGVSVAEQDPF
                     LG"
     gene            460..1356
                     /locus_tag="AC16G0_13250"
     CDS             460..1356
                     /locus_tag="AC16G0_13250"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR5100618.1"
                     /translation="MVKRNGPPARLRGVVTLALAWWLFQASVGCTLGGGPESEGPGHR
                     PQRLALTPEQELAVGREAYREILAKADILPPDHPAVDRVRRVGRRIVAATQIRPLLRE
                     INLHEEGYRFEWEFNVIDSDRVNALCLPAGKVVVFTGLLGFVRSDDELATVLGHEMGH
                     ALAHHASERLALSPADVEALRAAGGRLRDVDESRRRSLLGVLDAGAALERLSYNRFQE
                     SEADHIGVFLMTFAGYDPEAALEFWGRMQGASAGSIRLPEILSNHPADARRLAQLRGW
                     VPLAEGGKRAYDRGEIAPGVSR"
     gene            complement(1373..1504)
                     /locus_tag="AC16G0_13255"
     CDS             complement(1373..1504)
                     /locus_tag="AC16G0_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100619.1"
                     /translation="MNQHSTIYSRVSSRAQDTASQEPDLKRWAEGDFTLESETDHCS"
BASE COUNT          305 a          565 c          635 g          346 t
ORIGIN      
        1 tacagtagaa cgttgctcgg tagttacggc catgggtcat ctcctgatgc tcgtgcttcg
       61 tccccagccc ctgggctcag cccgccaagg gacttgccat ctacgcgagc ggggaaatag
      121 gtgcaaagcc gatgccgaga tggttctact tgttataaaa taactcgata tccataatta
      181 gcaagattta tgctccgatg gctttcggtg gaatgatagt tgcaaggaac ggtggggtgg
      241 ttcactggaa ctctgcggac caggagagag ggctgaaatg gacgagtgtc ccgcgggact
      301 cagtggtgcg cactggcgcg ccgcgatccg ggctcgacgg gcgcggcgtg ggatcaggaa
      361 ggacgatttc gagcggcgcg gggtcagcgt cgccgagcag gatccattcc tcgggtaatt
      421 ctcagccacc cagacggcgt tcatcggggg agccttgtca tggtgaagag gaacggtcca
      481 cccgctcgac tgcggggcgt ggtgaccctt gcgttggctt ggtggctgtt ccaggcgtcg
      541 gtcggatgta cgctcggagg cgggcccgag agcgaggggc cgggccatcg cccgcagcgg
      601 ctggccctca cgcccgagca ggagctggca gtgggacgcg aggcgtatcg ggagatcctg
      661 gcgaaggctg acatactgcc gccggaccac ccggcggtag atcgcgtccg acgggtaggc
      721 cgccggatcg tcgcggcgac tcagatccga ccgcttcttc gggagatcaa ccttcacgag
      781 gaggggtatc gcttcgagtg ggagttcaac gtcatcgact cggaccgcgt caacgcgctc
      841 tgcctgcccg caggcaaggt cgtcgtcttc accgggctgc tgggattcgt ccgcagcgac
      901 gacgagctgg caacagtgct cgggcacgag atggggcatg ccttggcgca ccacgccagc
      961 gagcggctcg cgttgagtcc cgcggatgtc gaggccttgc gggcggcggg cggtcgcttg
     1021 agagacgttg atgagtcccg ccgtcgatca cttctcggtg tgctggacgc cggggccgcg
     1081 ctggagcgcc tctcgtacaa ccggttccag gagtcggagg ccgaccacat cggcgtcttc
     1141 ctcatgacct tcgcgggcta cgatcccgag gcggccctgg agttctgggg gcggatgcaa
     1201 ggggcctccg ccggctcgat ccggctcccg gagatcttgt cgaatcaccc cgccgacgca
     1261 cggcgcttgg cccagctccg cggttgggtc ccgctcgccg aggggggcaa acgggcctac
     1321 gaccgaggcg agatcgcccc cggggtctcc cgctaaatcg tctcacgggg tgtcagctgc
     1381 agtggtccgt ttcggactcc agagtgaagt cgccctcggc ccaacgcttc aggtccggct
     1441 cctgggatgc cgtgtcctgc gcccggctcg acaccctgga gtagatcgtg ctgtgctggt
     1501 tcatcatctc ccccgtgttg tggcggaccc acgagggagg ataggccggg ggcgggcggt
     1561 cgtcaaggaa atgttctaac attcactagc cagtttacag tctgtaaggc ccccatcggg
     1621 cccaccggat cgtgcatttt gcacgatgac ccaccggaag cctgggccgc tcgagcgtcc
     1681 cgggcccact ccaggttgga cacctggtgc gtaggactat gccggcagtc aataggtgca
     1741 ctcgttggac acctggttcg tgggacacga gcggaacagc cattctgggc tggccccgag
     1801 ggtatcgagc cggttgcatt agtcccttcc caaccctcgc actgtccgtc t
//
LOCUS       DBNGZC010000773         1861 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2022034, whole genome shotgun sequence.
ACCESSION   DBNGZC010000773 DBNGZC010000000
VERSION     DBNGZC010000773.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2022034"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..1476
                     /locus_tag="AC16G0_13260"
     CDS             76..1476
                     /locus_tag="AC16G0_13260"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HIR5100620.1"
                     /translation="MAQFTTPRLRSQIHRLRNQPAPQPALGLEEVLPKATIEQVLQEE
                     GGKWKSILYTPWVTFWTFFWQVLSPDRSCRAALKRLAAWMGLRGGKLDDEDTSPYCKA
                     RARLPESALRRLMRARARESHQEAPAEWRWCGRNVKAVDGSTVIMADTPANQRAYPQN
                     PSQKPGLGFPIARFVVLFCLATGSALEMAIGQYCGKQTGENALFRSLWDELEPEDVVL
                     GDRYYCSYFDLVMLKQRGVDGVFRLHQRRPCDFRRGRRLGKEDQIVTWYRPRRPDWMD
                     EATYHQIPELMEIRQVRIHVGTPGFRTQVFDLATTLLDPELYTKSDLGMLFRQRWHAE
                     LDLRSIKIVLGMDLLRCKTPEMVRKEMWMTLLGYNVIRPLMAAASKEHGQDPRHVSFK
                     GALQTVQEFAVGLREGTPQQRRWLWRILLSSIAGDAVGYRPDRVEPRARKRRPKKYPL
                     LTKPRRQAQAALRKTG"
     gene            1519..1653
                     /locus_tag="AC16G0_13265"
     CDS             1519..1653
                     /locus_tag="AC16G0_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100621.1"
                     /translation="MKAVNGQDDVIDAFVLAAHSLEVLLARGEHGLIALDVESDAGLG
                     "
BASE COUNT          334 a          540 c          644 g          343 t
ORIGIN      
        1 gggggggtga aacgagacct cacttgtcgg atgatggggg tgttgagggc cctaccaccc
       61 gcaaggaggt ctcctatggc tcagtttacc acaccacggc tccgctccca aatccatcgc
      121 ctccgcaacc agccggcacc ccagcccgcc ttgggtctcg aggaggtcct ccccaaggcc
      181 accatcgagc aggtcctcca ggaggagggg ggcaaatgga agtcgatcct ctacaccccc
      241 tgggtgacct tctggacctt cttctggcaa gtcctcagcc ccgatcgctc ctgccgggct
      301 gccctcaagc ggctcgccgc ctggatggga ttgcggggtg ggaagctcga cgacgaggac
      361 accagcccgt attgcaaggc ccgcgcccgg ctgcccgaat cggcgctgcg tcgtctgatg
      421 cgagcccgcg cccgggagtc gcaccaagag gcgcccgcgg agtggcggtg gtgcggtcgc
      481 aacgtcaagg cggtcgatgg cagcaccgtg atcatggccg acactccggc caaccagagg
      541 gcgtatcccc agaacccctc tcagaagccc ggcctgggct tcccgatcgc ccggttcgtg
      601 gtgctcttct gcctggccac cggctcggcg ctggagatgg ccatcggcca gtactgcggg
      661 aagcagacgg gggagaacgc cttgtttcgc agcctctggg acgagttgga gccggaggac
      721 gtggtcctgg gcgatcgtta ttattgttct tattttgatc tcgtgatgct gaagcagcgg
      781 ggggtggacg gcgtgttccg gctgcatcag cggcgaccct gtgacttccg ccggggccgg
      841 cggttgggga aggaggatca aatcgtgacg tggtatcggc cccggcgtcc ggactggatg
      901 gatgaagcga cctaccatca gatccctgaa ctgatggaga tccgccaagt gcggatccac
      961 gtggggacgc cgggcttccg cacccaagtg ttcgatctcg ccacaaccct cttggatccg
     1021 gaactgtaca cgaaatcgga tcttggtatg ttgttccgac agcggtggca tgcggagctg
     1081 gatctgcggt caatcaagat cgtgctgggg atggacctgc tgcggtgtaa gacgccggag
     1141 atggtgcgta aggagatgtg gatgacgctg ttgggataca atgtgatccg gccgctgatg
     1201 gcggccgcgt cgaaggagca cgggcaggat ccgcggcacg tgagtttcaa aggggcgttg
     1261 cagacggtgc aggagttcgc ggtgggcctg cgggagggaa cgcctcagca acggcggtgg
     1321 ttgtggcgga tcctgttgag ctcgatcgca ggcgacgccg tggggtatcg acctgaccga
     1381 gtggaacctc gggcgcggaa gcggcggccc aagaagtacc cgttgttgac gaaaccccgg
     1441 aggcaagccc aagccgcctt gcgcaaaaca ggttagagct acaggacgtg ccattcgggt
     1501 ctcgtgccct ttctcgggtt gaaggccgtc aatggtcaag atgatgtcat cgacgccttc
     1561 gtattggcgg cgcatagcct cgaagtcctg ctggcgcgcg gcgagcatgg tctgatagcg
     1621 ctggacgtag agagcgatgc agggctcggt tagctcaatg gcgtaggagt cgatcagttc
     1681 gtgacgaatc tggggaatcg accagtgtcg cgcgaaccgc cgatgcccga tccagcagaa
     1741 gacgtcccac ccgatggccc aggcgggcag agcgatcgtc gcctccatct cggggctctt
     1801 ggtcttggcg tgccctgggc aatcaggatt ggggcagtga ttgagcttgc agacgagttc
     1861 g
//
LOCUS       DBNGZC010000774         2468 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_166155, whole genome shotgun sequence.
ACCESSION   DBNGZC010000774 DBNGZC010000000
VERSION     DBNGZC010000774.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_166155"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..347
                     /gene="rpsJ"
                     /locus_tag="AC16G0_13270"
     CDS             39..347
                     /gene="rpsJ"
                     /locus_tag="AC16G0_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIR5100622.1"
                     /translation="MDNQKIRIKLKAYDHRLLDQAVKDIVGTVQRSGALISGPIPLPT
                     KISRYTVLRSPHVDKKSREQFEIRTHMRMMDIVQPTPQTVDQLMRLDLPAGVDVEIKL
                     "
     gene            412..1041
                     /gene="rplC"
                     /locus_tag="AC16G0_13275"
     CDS             412..1041
                     /gene="rplC"
                     /locus_tag="AC16G0_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIR5100623.1"
                     /translation="MAIGLLGRKIGMTQVFDERGKAVPVTIIQAGPCPVVQKKTAERD
                     GYEALQIGFQSEPKATRLTRPLRGHFDKVQVPYQRHLSEIRLRGGEGEFQVGQVLTVG
                     VFAVGDRVRVTGVTKGRGFQGGVKRWNYRGGPETHGAMFHRAPGSIGASSYPSRVFRG
                     HHLPGHMGAERCTVQGLRVVSVDAEKHLLLVEGAVPGPSGGLLTIRKGK"
     gene            1089..1718
                     /gene="rplD"
                     /locus_tag="AC16G0_13280"
     CDS             1089..1718
                     /gene="rplD"
                     /locus_tag="AC16G0_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR5100624.1"
                     /translation="MTTMLDVVNAKNEKVDSLELPEAIFAASVKNHVLHEVVRMQLAS
                     RRQGTSDTKGRSEVSGGGKKPWKQKGTGRARAGTTRSPLWRHGGTIFGPHPRSYAYEV
                     PKAVRRQALLAALTTKAQAGAIRLLESVSLEKPSTKQMRGMLQTVGVQGKALVVLPAR
                     DEVVEKSARNLPEVRVLTVRGLNVYDVLKADTLVLTPEGVHTLREVLAP"
     gene            1715..2005
                     /locus_tag="AC16G0_13285"
     CDS             1715..2005
                     /locus_tag="AC16G0_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR5100625.1"
                     /translation="MKGPYEVVLRPLLTEKGTRLKEDGNQYLFRVAKTANKVEIKHAI
                     EHLFKVTVLEVRTIQQRGKVKRLGRFQGRRPDWKKAIATLKAGESIELYEGV"
     gene            2030..>2468
                     /gene="rplB"
                     /locus_tag="AC16G0_13290"
     CDS             2030..>2468
                     /gene="rplB"
                     /locus_tag="AC16G0_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR5100626.1"
                     /translation="MPVRKYRPTSAGRRFQSVSSFEEITKPTPEKSLLIPLNRTGGRN
                     ALGRLTCRHQGGGHKRMYRVIDFRREKHGIPATVAAIEYDPNRSSRIALLFYADGEKR
                     YILAPLGLGVGDRVMSGPEAEVRIGNALPLRNIPVGVTVHNIEL"
BASE COUNT          462 a          762 c          881 g          363 t
ORIGIN      
        1 aggacaggcg gcgctggaat caacggacag ggtgggtgat ggacaaccag aagattcgga
       61 tcaagctcaa agcctacgat caccgactgc tggatcaggc agtgaaggac atcgtgggaa
      121 ccgtccagcg ctcgggcgcc ctgatttccg gccccatccc gctgcccacg aagatcagcc
      181 gctacacggt gctccgctcg ccccacgtgg ataagaagtc ccgggagcag ttcgagatcc
      241 ggacccacat gcggatgatg gacatcgttc agcccacccc gcagacggtg gaccagctca
      301 tgcggctgga tttgccggcg ggtgtggatg tggagatcaa actctaagca gcgatcccgc
      361 gatccgcgcc ccggagacgg gggcggtggc gggcatccgg cgcgtgcgac catggcgatc
      421 ggactcttgg gacgcaagat cgggatgacg caggtatttg acgagcgcgg gaaggccgtc
      481 ccggtgacga tcatccaggc cgggccctgt cccgtggtgc agaagaagac ggccgagcgg
      541 gacggctacg aggccctgca gatcggattc cagtccgagc ccaaggcgac ccggctgacg
      601 cggccgctgc ggggccactt cgacaaggtg caggtgccct accagcgcca cctgagcgag
      661 atccgcctgc gtggcggtga gggcgagttc caggtgggcc aggtcctcac ggtgggcgtc
      721 ttcgcggtgg gcgaccgggt gcgcgttacg ggggtcacca agggccgcgg cttccagggc
      781 ggggtgaagc gctggaacta tcggggcggt ccagaaaccc atggcgccat gttccaccgg
      841 gcaccgggct ccatcggggc gtcctcctac ccgtcccggg tttttcgcgg ccaccacctg
      901 cccggccaca tgggggccga gcgctgcacg gtgcaggggt tgcgagtggt gtcggtggac
      961 gccgagaagc acctcctgtt ggtggagggg gcggtgccgg gaccaagcgg cgggttgctc
     1021 acgattcgaa aaggcaagtg aggacgggtg actctcctgc cgatccggcc gggaggcctc
     1081 gcgagagcat gacgacaatg ctggatgtgg tgaacgcgaa gaacgagaag gtggacagcc
     1141 tggagctgcc ggaggcgatc ttcgccgcgt cggtaaaaaa tcacgtcctg cacgaggtgg
     1201 tgcggatgca gctggccagc cgccgacagg ggaccagcga caccaagggg cgctctgagg
     1261 tgtccggggg cggcaagaaa ccctggaagc agaaggggac gggccgtgcc agggccggaa
     1321 cgacccgctc gcccctgtgg cggcatggcg ggacgatctt cggtccccat ccccgctcct
     1381 acgcctacga ggtccccaag gctgtgcgcc gccaggcgct cctggcggcc ctgaccacca
     1441 aggcccaggc gggagccatt cgcctgctgg agtcggtcag cctggagaag cccagcacca
     1501 agcagatgcg gggcatgctc cagacagtgg gggtgcaggg gaaggccctg gtggtgctgc
     1561 cggcgcggga tgaggtggtg gagaaatcgg cgaggaacct gcccgaggtg cgcgtgctca
     1621 cggttcgggg cttgaatgtc tacgacgtcc tgaaggcgga cacgctggtt ctgacccccg
     1681 agggggtgca cacgctgcgt gaggtgctgg cgccatgaag ggaccatacg aggtcgtgct
     1741 gcgtccgctc ctcaccgaga aggggacgcg cctcaaggag gatgggaacc agtacctctt
     1801 ccgtgtggcc aagacggcca acaaggtcga gatcaagcac gccatcgagc acctcttcaa
     1861 ggtcaccgtg ctggaggtgc gcacgatcca gcagcggggg aaggtgaagc ggctggggcg
     1921 attccagggt cgccggccgg actggaagaa ggccatcgcc accctgaagg cgggtgaatc
     1981 catcgagttg tacgagggcg tgtagcgagc caggagcaag gattcggcca tgccagttcg
     2041 caagtatcga ccgacatcgg cgggacgccg cttccaaagc gtctccagct tcgaagagat
     2101 cacgaagccc acgcccgaaa agtctctgct gattcccctg aaccggaccg ggggacgaaa
     2161 cgccctgggg cggctcacct gccgacacca gggcggcggg cacaagcgga tgtatcgcgt
     2221 gatcgacttt cgccgggaga agcacggcat cccggccacc gtcgccgcca tcgagtacga
     2281 ccccaaccgg tcgtcccgaa tcgcgctcct cttctatgcc gacggggaga agcgctacat
     2341 cctggccccc ctgggtctcg gcgtcgggga ccgcgtcatg tccggtcccg aggcggaagt
     2401 ccggatcggc aacgcccttc cgcttcggaa catccccgtc ggggtgaccg tgcacaacat
     2461 tgaactct
//
LOCUS       DBNGZC010000775         1677 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1829976, whole genome shotgun sequence.
ACCESSION   DBNGZC010000775 DBNGZC010000000
VERSION     DBNGZC010000775.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1829976"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="AC16G0_13295"
     CDS             <1..444
                     /locus_tag="AC16G0_13295"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl trahydropterin synthase family
                     protein"
                     /protein_id="HIR5100627.1"
                     /translation="VIQLTKRIEFCAAHRYANPRWSAEENARVFGPCVNLHGHNYVLE
                     VTIQGEPAPETGMVMDLKVLKDLILETVMDRFDHKNLNVDVPLFADTIPTPENLAVVI
                     WELLAPQLKGCSLARIRLHEDGTTYVDYIGERGVAPETTGAGRAD"
     gene            434..940
                     /locus_tag="AC16G0_13300"
     CDS             434..940
                     /locus_tag="AC16G0_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HIR5100628.1"
                     /translation="MPTDPRGLPLVEMTRTYQFSAAHRMHNPAFSDAENARLYGRCNN
                     PTGHGHTYRLTVTVRGPVSPDTGWVMPAEALDALVRQQVLTRFDRANLDQLLSPADGV
                     TSTTEVLAGLLWRLLEGPLAGAMLWRLRIEETSSNFFEINRPLLQRAAVPPDRPGPKA
                     GRQKASRP"
     gene            937..1491
                     /gene="folE"
                     /locus_tag="AC16G0_13305"
     CDS             937..1491
                     /gene="folE"
                     /locus_tag="AC16G0_13305"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HIR5100629.1"
                     /translation="MMEPLVRDLLKELGEDPYREGLQKTPERVAKAMRYLTSGYEKTV
                     ADVLNDAIFTEPYDEMVVVKDIEVYSLCEHHLLPFFGKAHIAYLPAGRIVGLSKLARL
                     VDMFARRLQVQERLTTQVAEALQEALRPAGVGVVIEAAHFCMMMRGVEKQNSMAVTSC
                     MLGAFRDSRMTREEFLQLIRPTHG"
BASE COUNT          299 a          574 c          545 g          259 t
ORIGIN      
        1 gtgatccagc tcacgaagcg aatcgagttc tgtgccgccc accggtatgc gaacccgcgc
       61 tggagcgcgg aggagaacgc ccgggtattc ggcccctgcg tcaacctcca cggccacaac
      121 tatgtgctgg aggtgacgat ccaaggcgag ccggcgcccg agaccgggat ggtgatggat
      181 ctcaaggtgc tgaaggacct gatcctcgag acggtcatgg atcgcttcga ccacaagaac
      241 ctgaacgtgg atgtcccgtt gttcgccgac accatcccca ccccggagaa tctggcggtg
      301 gtgatctggg agctgctggc gccacagctc aagggctgca gcctggcccg catccggctc
      361 cacgaggatg gcaccaccta cgtggactac atcggcgagc gcggcgtagc accagagacg
      421 acgggggccg gccgtgccga ctgatccgcg gggactgccc ctggtcgaga tgacccggac
      481 ctaccagttt tccgccgccc accggatgca caatccggcc ttcagcgacg ccgagaacgc
      541 gcgtctgtac ggccgctgca acaaccccac cggccacggc cacacctacc gcctgacggt
      601 cacggtccgc ggtccggtgt cacccgacac cgggtgggtg atgccggccg aggcgctgga
      661 tgcgctggtg cgccagcagg tcctgacccg cttcgatcgg gcaaacctcg accagctcct
      721 cagtcccgcg gacggagtca ccagcaccac cgaggtgctg gccgggctcc tctggcgtct
      781 cctggagggc ccgctggccg gggcgatgct gtggcggctg cgcatcgagg agacgtccag
      841 caacttcttc gagatcaacc gcccgctgct gcagcgcgcg gccgttccgc cggatcggcc
      901 ggggcccaag gcagggaggc agaaagcgag tcgcccatga tggagccact ggtgagagac
      961 ctgctcaagg aactcggcga agacccctat cgcgaagggc tgcaaaagac gccggagcgg
     1021 gtggccaagg cgatgcgcta cctcacctcg gggtacgaga agaccgtggc cgacgtcctc
     1081 aacgatgcca tcttcaccga gccgtacgac gagatggtcg tggtcaagga catcgaggtc
     1141 tactcgctct gcgagcacca cctgctgcct ttcttcggca aggcccacat cgcgtacctg
     1201 cccgcgggcc ggatcgtggg gctgagcaag ctggcccgcc tggtggacat gtttgcccgc
     1261 cggctccagg tgcaggagcg gctcaccacc caggttgcgg aggcattgca ggaggccctc
     1321 aggcccgccg gggtgggtgt ggtcatcgag gccgcccact tctgcatgat gatgcgcggc
     1381 gtggagaagc agaacagcat ggccgtcacc tcgtgcatgc tgggcgcctt ccgggacagc
     1441 cggatgaccc gcgaggagtt cctgcagctg atccggccca ctcacggcta gggcctcgag
     1501 cgccggttga cacatctcca ttcatctgct atctaaagcc atgggtagca attcacatca
     1561 acctccggat tgaggatgca caaacccacc gacccccgcc gcggggggct gaccctgccg
     1621 gtggccccgc gcgatcccgg accatcctcc cccatcggtg tggtcgccga gctcctc
//
LOCUS       DBNGZC010000776         4727 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_751733, whole genome shotgun sequence.
ACCESSION   DBNGZC010000776 DBNGZC010000000
VERSION     DBNGZC010000776.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4727)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_751733"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="AC16G0_13310"
     CDS             complement(<1..358)
                     /locus_tag="AC16G0_13310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HIR5100630.1"
                     /translation="MVTIESTPEMVAKVYEKMARNLETVRRRLGRPLTLADKVVLGHL
                     DDPAHQDMEPGKSYLFLRPDRVIFQDVLGQTGMLQFMQTRREKVAVPTTIHCDHLVQA
                     RVEGQADLRESLEENKE"
     gene            complement(509..1396)
                     /locus_tag="AC16G0_13315"
     CDS             complement(509..1396)
                     /locus_tag="AC16G0_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009675862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5100631.1"
                     /translation="MEAVTTLPERDGLAAPALRKRSGVLRRFGRNPLAVAALGYLGLV
                     ILAAVFAPVITPHDPNRIVMGETLVPPSVGHVLGTDESGRDEFSRLLHGARISLTIGV
                     VAMLLSVTLGTALGALAGLFGGIVDALTMRLTDAMLSIPIFFFLLTVLAVFGSTVPII
                     IIAIGLSSWMTAARVVRAEVLRTINLEYVLAARGLGAGSWRIALLHLLPSAVPSMLVA
                     STIGVGQAILTESALSYLGVGVQPPVASWGNMLSNAQNYFFPAPFLAIWPGMLILFTV
                     LSFNCIGDSARDTLSPYQN"
     gene            complement(1410..2357)
                     /locus_tag="AC16G0_13320"
     CDS             complement(1410..2357)
                     /locus_tag="AC16G0_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012960480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5100632.1"
                     /translation="MLRYVMNRLVQSVVLVVFVSMLTFFLIHSAPGGPAVLLAPDMTK
                     AQIEQARKELGLDDPVPVQYARWAGHVARGNMGTSYSQGLPVTDLLVRRLPETLELVF
                     TGLALAAITGVVIGIVSARKPYSLLDKLSTGFCFFGMSVPVFWLGLMLIIFFSITLKI
                     LPSAGSYTLGAPASILDRLAHLILPAVVLATANLALIARYTRSSMLEVLNADYLRTAR
                     AKGVKESVVMNRHALRNALIPIVTVVALMVPRLVSGVAITESVFAWPGMGSLAVDSAV
                     QRDYPVVMGITLMVSVLVVVTNFITDLSYLFLDPRVKVK"
     gene            complement(2364..3743)
                     /locus_tag="AC16G0_13325"
     CDS             complement(2364..3743)
                     /locus_tag="AC16G0_13325"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HIR5100633.1"
                     /translation="MGPTEALARFAVEMDGRSLSSQVVHAGKRAILDCIGVTLAGSSE
                     PAARIVAAQVLSHGGAAESVIWGAEGARTSAQNAALANGTAAHVLDYDDYILAMPGHP
                     SVPVLPAALAVGERLHASGAAVLASFLVGFEVETKLGAFSGGQAYDVGWHPTSTLGVI
                     GATAAVGRLLGLGLPEMRYALGIAVSMAAGVRQNFGSMTKPFHVGRAAQSAILAAELA
                     QRGFTSSQEAIEGQFGFWDVFAGALNRDGDALARTLGNPFAVAKPGVSYKAWPCCAST
                     FGGVDAALQIAGGLSNPGIDSVTVDVPYTAPLILIHHRPTTPLAAKFSLEYCVAAALL
                     DGRVTLGHFTQEAVRRPDLQSLLRRIDYRVPEDWQKGAGTWKQGHGRVEVRLRDGSVR
                     RGVTSAPKGDAANPMSDEELEAKFLECASLALGEGRAKDALELIRSLERLDDVGRLTD
                     TLAGAGAAR"
     gene            complement(3882..>4727)
                     /locus_tag="AC16G0_13330"
     CDS             complement(3882..>4727)
                     /locus_tag="AC16G0_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5100634.1"
                     /translation="ADGPGHLSGRPIVFFGMRGNLKMELEATGANRDLHSGNFGGPVP
                     NPVWALVDLLGSMRFPDGRVTIEGFYERVVPPTAYEREVMRRMPFDEAAFKENLGIAE
                     FAGPKDLSYYEKIMFQPMLNITGIAGGYAGKGAKSAIPSKAVVKLETRMVHDMEPEDT
                     FSKIQRHVQRYAPNVAIRKLAATRPSKTSLDLPVSRMVIGAISDAYGVEPVVQPVLGG
                     SSPNYLFTNVLKLPAIWVTYGPHDQNNHAPNENITVKSFLDGIRASALVLQRFAAMPG
                     EHGRG"
BASE COUNT          878 a         1607 c         1476 g          766 t
ORIGIN      
        1 cctccttgtt ctcttcgagt gactcgcgca gatcggcctg gccctcaacc ctcgcctgga
       61 cgagatggtc gcaatggatg gtcgtgggca cggcgacctt ctcccgccgg gtttgcatga
      121 actgcagcat gccggtctgg ccgagcacgt cctggaaaat gacgcggtcg ggacggagga
      181 acagatagct cttgccgggc tccatgtcct gatgcgccgg gtcgtccagg tgccccagca
      241 cgaccttgtc cgccaaagtc agcggtctgc ccaaccggcg gcgaaccgtc tcgagattcc
      301 gcgccatctt ctcgtacacc ttggccacca tttccggagt cgattcgatc gtgaccattc
      361 tcttgccctc ctcgtgctcc atggacgcgc tcacacgcgc caggggacgc accctgttgt
      421 tgacccaccc gaacgatcat ccgatgcgct ggcgatggtg ttctccttcg atcgatgcaa
      481 ggctccacag ccggaccgcc ggccgggctc agttctgata cgggctcaag gtgtcacgcg
      541 ccgagtcgcc gatgcagttg aaactgagca ccgtgaacag gatcagcatg cccggccaga
      601 ttgccaggaa gggggccggg aagaaatagt tctgggcgtt gctcagcatg ttcccccacg
      661 aggcgacggg cggctgcacc cccaccccca ggtagctcag cgccgactcg gtgagaatcg
      721 cctgccccac gccgatggtc gaggccacca gcatggaggg gacggccgag ggcaacaggt
      781 ggagcagggc gatccgccag ctcccggcgc cgagccctct ggccgccaag acgtattcca
      841 ggttgatcgt gcgcaagacc tcggcccgca cgacgcgggc cgccgtcatc cagctcgaca
      901 gtccaatggc gatgatgatg atcggcacgg tcgatccgaa caccgccagg acggtcaaca
      961 ggaagaagaa gattgggatg gacagcatgg cgtcggtcag acgcatcgtc agcgcatcca
     1021 cgatcccgcc gaacaggccg gccagcgccc ccagggcggt cccgagtgtc accgaaagca
     1081 gcatggccac cacgccgatg gtcaatgaga tgcgagcacc gtggagcagg cgactgaatt
     1141 cgtcccggcc gctttcatcg gtccccagca cgtgccccac cgacggcggc accagcgttt
     1201 ctcccatcac gatccggttg ggatcgtggg gcgtgatcac cggcgcgaag accgccgcca
     1261 ggatgaccag gcccagatac cccagggcgg ccaccgccaa gggattgcga ccaaaccggc
     1321 ggaggacgcc cgaacgtttt cgcagcgccg gcgcggccag gccatcacgt tccgggagtg
     1381 tggttaccgc ttccaccgtt ccatccagct cacttgacct tcacccgagg atccaggaag
     1441 agatagctga ggtcggtgat gaaattggtg accacgacca gcaccgagac catcagcgtg
     1501 atgcccatga ccaccgggta gtcccgctgc accgcggagt cgacagccag cgagcccatt
     1561 ccaggccagg cgaacacgct ctcggtgatg gccacgccgc tcaccaggcg cgggaccatc
     1621 agcgccacca cggtcacgat ggggatcagg gcattccgca gggcatggcg gttcatgacc
     1681 acgctctcct tcacgccctt ggcccgagcc gtccggaggt aatcggcgtt caggacctcg
     1741 agcatgctgg atcgcgtata gcgagcgatc agggccaggt tcgccgtcgc caggacaacg
     1801 gccgggagga tcagatgcgc aagccgatcc agaatcgatg ccggggcacc cagggtgtag
     1861 gacccggcgg agggcaggat cttcagcgtg atggagaaga agatgatcag catcagcccc
     1921 agccagaata ccggaaccga catgccgaag aagcagaatc ccgtcgacag cttgtccaac
     1981 agcgaatacg gcttccttgc cgacacaatc ccgatcacca ctccagtgat ggccgccagc
     2041 gccagtccgg tgaacaccag ctccagcgtc tccggcagcc gcctgaccag gagatcggtc
     2101 acgggcaagc cctggctata ggaggtgccc atgttgcccc gcgccacgtg gcccgcccag
     2161 cgagcatact gcacgggcac ggggtcgtcg aggcccagtt ccttgcgcgc ctgttcgatc
     2221 tgcgccttcg tcatgtccgg cgccagcaac acggccggtc cacccggcgc cgagtggatc
     2281 aagaagaagg tcagcatgct cacaaagacg accagcacaa ccgattgaac cagtcgattc
     2341 atcacgtacc tgagcattcg gtcctacctc gccgctccgg cccctgccag ggtgtcggtc
     2401 aggcgcccca catcgtcaag gcgttcgagg gatcgtatca gctccagggc gtccttcgcc
     2461 cgtccctcgc cgagcgcgag gctcgcgcac tccaggaact tggcctccaa ttcctcgtcg
     2521 ctcatcgggt tggccgcatc gcccttgggc gcgcttgtca cgccccggcg gacgctcccg
     2581 tccctgagcc gcacctccac gcgaccgtgc ccctgcttcc acgtgccggc ccctttctgc
     2641 caatcctcgg gcacacggta gtctatccgc cggagcaacg actggagatc gggacgccgt
     2701 accgcttcct gggtaaaatg gcccaaggtc acgcgtccgt ccaggagcgc cgcggccacg
     2761 cagtactcca gggagaactt ggcggccagg ggtgttgtgg ggcgatggtg gatcaagatg
     2821 aggggcgccg tgtaggggac atccaccgtc acgctgtcga tgccgggatt tgacagcccg
     2881 ccggcgatct ggagcgcggc gtccacgccc ccgaacgtcg aggcgcagca gggccaggcc
     2941 ttgtagctca cccccggctt cgccacggca aaggggttcc ccagggtccg cgccagggcg
     3001 tcgccatccc ggttcagggc gccggcgaac acatcccaga aaccgaattg tccctcaatg
     3061 gcctcctggc tggaggtgaa accgcgctgc gccaactcgg cggcaaggat cgccgactgg
     3121 gctgcccgcc ccacatggaa cggtttggtc atgctgccaa agttctggcg aacgccggcc
     3181 gccatggaga cggcgatgcc cagggcatag cgcatctcgg gaagacccag acccagcagt
     3241 cggcccacgg cggcggtggc gccgatcacc ccgagggtgc tggtgggatg ccaccccaca
     3301 tcgtaggcct gcccccccga gaacgccccc agcttggttt ccacctcgaa gccgacgagg
     3361 aacgatgcca ggacagcggc cccggaggcg tggaggcgct cgcccacggc cagcgccgcc
     3421 ggcaggaccg gcacgctcgg atgtcccggc atggccaaga tatagtcgtc gtaatccaag
     3481 acgtgagccg ccgtcccatt ggccagggca gcattctggg cgctggtccg tgccccctcg
     3541 gccccccaga tgacgctctc ggcggcaccc ccgtggctca agacctgggc ggcgacgatc
     3601 cgcgcggccg gctccgacga gcccgccagc gtcacgccga tgcaatccag gatggctcgt
     3661 tttccagcgt gcaccacctg ggaagacagt gatcgcccat ccatctcaac cgcaaatcgc
     3721 gccagagcct cggtgggtcc catcaaatca cctcgagaat gaccaatgac caaatcccaa
     3781 atcccaatac ggcccctatt ctggcagcaa cgtcactcgt cggaaacggt agcggatccc
     3841 cgtttggtca ttggtcattt ggattttgtc attgcccgat atcatccccg gccgtgctcg
     3901 ccgggcatgg cggcaaaccg ctgcagcacg agggcgctcg cgcgtatgcc atccaagaag
     3961 gacttgacgg tgatgttctc gttgggcgcg tggttgttct ggtcgtgggg accataggtt
     4021 acccagatgg ccggcagctt cagcacgttg gtgaaaaggt aattgggact ggacccgccg
     4081 aggacaggct ggaccaccgg ttccaccccg taggcgtcgg agatggcccc gatcaccatc
     4141 ctggacacag gcaggtccag cgaggtcttg gacggtcgcg tggcggccag ctttcggata
     4201 gcgacattgg gcgcgtagcg ctggacgtgg cgctggatct tggaaaaggt atcttccggt
     4261 tccatgtcgt gcaccatccg tgtctccagc ttcaccaccg ccttggaggg gatggccgac
     4321 ttggccccct tgcccgcata gccgccggcg atgccggtga tgttgagcat cggttgaaac
     4381 atgatcttct cgtagtacga cagatccttg gggccggcga actcggcgat gccgagattc
     4441 tccttgaagg ccgcctcgtc gaagggcatc cgtcgcatca cctcgcgctc gtaggcggta
     4501 ggcggcacca cccgctcgta gaagccctcg atggtcaccc gcccatcggg gaagcgcatg
     4561 ctgccgagaa gatcgaccag cgcccagacg gggttcggca ccggtccgcc gaagttgccg
     4621 gagtggaggt cgcgattggc acccgtcgcc tccagctcca tcttgagatt gccccgcatc
     4681 ccgaagaaga cgatgggccg gccggagagg tgacccggcc cgtcggc
//
LOCUS       DBNGZC010000777         2458 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_850528, whole genome shotgun sequence.
ACCESSION   DBNGZC010000777 DBNGZC010000000
VERSION     DBNGZC010000777.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_850528"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..1215
                     /locus_tag="AC16G0_13335"
     CDS             73..1215
                     /locus_tag="AC16G0_13335"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR5100635.1"
                     /translation="MKRLSILGSTGSIGTRTLEVVEAHPDHFQVAALAAQQNLDLLES
                     QVRRHSPRVVSVGSEASARELRRRLGGNRTEVSWGKAGLQGAAADSGADLVVSAIVGG
                     AGLPPTAAAVEAGKNVALANKESLVMAGEILTADARRRGVRILPVDSEHSAIYQCLSQ
                     GQPTEVRRIVLTASGGPFRARAGATFAAITPEEALRHPTWSMGKKISIDSATLMNKGF
                     EVIEAHWFFGVPVERVDVVIHPQSIVHSLVEFVDGSLLAQLGVPDMRIPIQYALTYPE
                     RRENRVPRLRLEAMGSLTFEAVDRDKFPCLDLAYEAATAGGSFPTVLNAANEVAVQRF
                     LDRRIGFDEIPTVIRKALDAHPRRRVGSVEEVLEVDREVRLWLEAR"
     gene            1315..>2458
                     /gene="rseP"
                     /locus_tag="AC16G0_13340"
     CDS             1315..>2458
                     /gene="rseP"
                     /locus_tag="AC16G0_13340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIR5100636.1"
                     /translation="MTILAAVVVLGILVFIHELGHFLVAKRSGVGVLKFSLGFGPKVV
                     GVKRGDTEYLISALPLGGYVKMIGEDPGDESPEAADPERSFSRKSVGTRARIILAGPL
                     ANLLFPVLIFWGVFTFAGQPYFLPIIGTPEKDSPAAQAGLAAGDRVKALDGQPIERWD
                     EIETAVAASSGRKLDLTLVRDGRTVEVSVEPRAIKTQDLFGQDIETWDLGLHPFLSTR
                     IGQVLPGHVAQQAGLKSGDRIVALDGAPVAEWDQLAKAIHASPGKPIRLGVERDGGRF
                     EVTVVPRATRQKGEASEEEIGLIGIGPAPESHYRRLNPYSAAIAGVKKTTDLSVLIVQ
                     GFVKLIQAKISPKTIGGPILIGQMAGEVVQRGPIELLSFTALLSINL"
BASE COUNT          401 a          763 c          871 g          423 t
ORIGIN      
        1 aaagttgagg accggtcggc tcccacagta gccgaggggt gtgccgttgt cagtgatgtt
       61 gcgggaggtg ggatgaagcg gctttccatt ctgggatcga caggctccat cgggacgcgc
      121 acgctggagg tggtggaggc tcaccccgat catttccagg tcgcggcact ggcggctcag
      181 cagaatctcg atctcttgga gtcgcaggtc cggcgccatt ccccccgcgt ggtctcggtg
      241 ggaagcgagg ccagcgcccg ggagctccgg cgtcggctcg gcggaaaccg gaccgaggtg
      301 agctggggaa aagcgggcct gcaaggggcc gctgcagact cgggggcgga cctggtggtg
      361 tcggccatcg tggggggagc gggactcccc ccgaccgcgg cggcggtcga ggccggtaag
      421 aacgtggccc tggccaataa ggagtccttg gtcatggccg gggagatctt gaccgccgat
      481 gcccggagac gcggggtccg gatcctgccg gtggacagcg agcattccgc catctaccaa
      541 tgtctgtccc agggccagcc gaccgaggtg cggcgcatcg tcctcacggc atccggaggc
      601 ccattccgag cccgagcggg tgcaaccttc gccgccatca caccggagga ggcgctgcgg
      661 catcccacct ggtccatggg aaagaagatc tccatcgact cggccacgtt gatgaacaag
      721 ggatttgagg tgatcgaggc ccactggttc ttcggcgtgc ccgtggagcg ggtggatgtg
      781 gtcatccatc cgcagagcat tgtccattcg ctggtggagt ttgtggacgg ctccctgctg
      841 gcccagcttg gcgtgccgga catgcgcatc cccatccagt atgccctgac ctatcccgag
      901 cgccgggaga acagggtgcc gcggctccgg ctcgaggcga tgggcagtct caccttcgag
      961 gccgtggatc gagacaagtt cccctgcctg gacctggcct acgaggcggc gacggccggc
     1021 ggctccttcc ccacggtgct gaacgccgcc aatgaggtgg cggtgcagcg gttcctggac
     1081 cggcgcatcg gcttcgatga gatccccacc gtgatccgga aggcactgga tgcccatccg
     1141 cggcgtcgcg tcgggagtgt tgaggaagtc ttggaggtgg accgggaggt ccgtctctgg
     1201 ttggaagcga ggtagaaccg cgagccacgg tttgcgagcc gcgagccatg aggtgcggcg
     1261 ggaatcctac gaggctcaag gctctgggct cgtggctcga cgaatggagt tcggatgacg
     1321 attctggcgg cggttgtcgt gctgggcatc ctggtcttca tccacgagct gggtcacttt
     1381 ctggtggcca agcgctcggg ggttggggtc ctgaagttct ctctgggttt tgggcccaag
     1441 gttgtcgggg tgaaacgcgg ggatacggaa tacctcatct ccgccctgcc gctcggcggg
     1501 tacgtcaaga tgatcgggga ggatcccggc gatgagtctc cggaggcggc cgacccggag
     1561 cggtccttct cccggaagag cgtcgggacc cgtgcccgga tcatcctggc cggaccgcta
     1621 gccaacctgc tcttccccgt cctgatcttc tggggggtct tcaccttcgc cggccagccc
     1681 tacttcctgc ccatcatcgg gacacccgag aaagattccc ccgcggcgca ggcagggctt
     1741 gccgccgggg atagggtgaa ggcgctggac ggccagccca tcgagcgctg ggacgagatc
     1801 gagacggccg tcgccgcctc atccggccgc aagctcgatc tcacgctggt ccgcgacggg
     1861 cggaccgtgg aggtcagcgt cgagccgcgg gcgatcaaga ctcaggacct gttcggtcag
     1921 gatattgaga cgtgggatct ggggctccac ccgttcctgt ccacgcggat cggccaggtg
     1981 ctcccgggcc acgtggccca acaggccggt ctcaagagcg gtgatcgcat cgtggcgctg
     2041 gacggggccc ccgtcgccga gtgggaccaa ctcgccaagg ccatccacgc cagcccgggg
     2101 aaacccatcc gtctgggcgt ggagcgggac ggggggcgct tcgaagtgac ggtggtccca
     2161 cgggccacgc ggcagaaggg cgaggccagt gaggaggaga tcggcctcat cggcatcggc
     2221 ccagcgccgg aatcgcacta ccgccggctg aacccgtact ccgcagccat cgccggcgtg
     2281 aagaagacca cagatctctc ggtgctgatc gttcaaggct tcgtgaagct gatccaggcc
     2341 aagatttcac ccaaaaccat cggcggtccg atcctcattg gccagatggc gggtgaggtg
     2401 gtccagcggg ggccgataga gctactctcc ttcacggcgc tcctctcgat caacctgg
//
LOCUS       DBNGZC010000778         2017 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_752017, whole genome shotgun sequence.
ACCESSION   DBNGZC010000778 DBNGZC010000000
VERSION     DBNGZC010000778.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_752017"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..>2017
                     /locus_tag="AC16G0_13345"
     CDS             9..>2017
                     /locus_tag="AC16G0_13345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HIR5100637.1"
                     /translation="MARALLGSPANHLSRLPEVSLDVSLPLEGWVVVPPDPRPAESRD
                     AGDPERTRFVAEVTRLRIGGTWVETTGQARLTILGETVEVAYGDDVRGTFRLRHPRRF
                     DNPGSFDYPRYLATQGIALEGWTRDPVEVFRASRGSPVLAAIFRLRGLLLQRLDAAMP
                     APEAALLKASILGDRTGLTPEMNQAFLDSGTYHILAISGLNVSLLAGALFGLLRLLRA
                     SSQIAAAASILLVSLYAALAGAGPSVIRAAVMADTYLLAVVLDRRADLLNSLALSALG
                     LLWWNPRNLFDVGFQLTYLATLGIILLLPRCDQALASLPRPIRWPLESVAITLAATIM
                     TLPVLAAAFNRIAPVGFLANIPIVPLSGLITGLGTAAGAIVLAIPGGLPWLNQLNGWL
                     VDALFISARWFASWPWSSLRVYTPTPGMLAAYYGLVGAWLLRTSPADGGGASARRRYV
                     ARWGTIVCGILLAVQVGLRLYPWEGPPRVRLTMLDVGQGEAIFLDLPGGRRMLVDAGS
                     LAGDAFDVGLRVVAPFLWHEWVGHLDALVLTHAEADHINGMPSILRSFSVDEVWAGDI
                     PTTSPTALWIQEYARHRRIPYRILGADTGPVQVGEARIEFLHPPPARGSVLEEGKGKR
                     SRSNATSRVLRVSLGNQALLLTGELERDGDSVRLAESRPIHAAVLT"
BASE COUNT          282 a          678 c          682 g          375 t
ORIGIN      
        1 ccgagagcat ggcccgtgcc ctcttaggaa gcccggcaaa tcacctgagt cgcctgccgg
       61 aggtcagcct ggatgtctcc ctgcctctgg aaggctgggt cgtcgtgcca cccgatccgc
      121 gtcccgccga atctcgtgac gcgggcgacc cggagaggac tcgcttcgtc gccgaggtga
      181 cccgcctccg catcggaggg acatgggtcg aaacgacggg acaggcgcgc ctgaccatcc
      241 tgggcgagac cgtggaggtt gcctacggcg atgacgtccg cggcaccttc cgcctgcggc
      301 atccgcggcg gttcgacaac cccgggagtt ttgattaccc ccgctatctt gccacccagg
      361 gaatcgccct ggaagggtgg acgcgggatc cggtggaggt gttccgggcg tcccgcggct
      421 cgccggttct ggcggccatc ttccgcctgc gaggcctgct gctccagaga ctcgatgccg
      481 ctatgcctgc gccggaggcg gccctgctga aggcgtcgat cctgggggat cgcaccggtc
      541 tcacgccgga aatgaaccaa gccttcctgg actcgggcac gtatcacatc ttggcgatct
      601 ccggactgaa tgtgagcttg ctggccggcg cgctgttcgg cctcctccga cttctgcggg
      661 cttcgtctca gatcgccgcc gcggcctcca tactcctcgt cagcctatat gccgccttgg
      721 caggcgccgg cccatcggtg atacgggcgg ccgtgatggc cgacacctac ctcctcgccg
      781 tggtcctgga ccggcgggcc gacttgctaa acagcctggc cctgtcggcc ctggggctcc
      841 tgtggtggaa tccccgcaac ctgttcgacg tgggcttcca gctcacctac ttggccacgc
      901 tcgggatcat cctgttgctg ccgcgctgcg accaggctct ggcgagcctt ccgcgaccga
      961 tccgctggcc cttggagtcg gtcgccatca cgctggcggc gaccatcatg acgctgcccg
     1021 tcctggcagc cgccttcaac cggatagccc cagtgggatt cctggccaac attccgatcg
     1081 ttcccctgag cgggctcatc accggcctgg gaacggccgc cggggccatc gtcctggcca
     1141 ttccgggggg gcttccgtgg ttgaaccagc tcaatggctg gctcgtggac gcgctcttca
     1201 tctccgcccg ctggtttgcc tcttggccgt ggagcagcct gcgggtctat acccccactc
     1261 ccggcatgct ggcggcgtac tacggcctcg tgggcgcatg gctcttgcgc acgtcgcccg
     1321 cggatggcgg aggagcatca gcgcgccgtc ggtatgtggc gcggtgggga acgatcgtct
     1381 gcggcatcct gctggcagtc caggtgggtc tacggcttta cccgtgggaa ggaccgccga
     1441 gggtgcggct caccatgctg gatgtgggcc agggggaggc catcttcctc gacttgcccg
     1501 gcggtcgtcg gatgctggtg gatgccggca gcctggcggg cgatgccttt gatgtcgggc
     1561 tgcgggtggt ggcgccgttc ctctggcatg aatgggtcgg ccacctggac gccctcgtgc
     1621 tcacgcatgc cgaagccgat cacatcaacg ggatgccctc gattctccgc agcttcagcg
     1681 ttgacgaggt gtgggcgggg gatatcccca cgacctcccc gacagccctt tggatccaag
     1741 agtacgcccg ccatcggagg attccgtatc ggatcctggg ggcggataca ggtccggtcc
     1801 aggtggggga ggcccggatt gagttcctgc accctccccc cgccaggggg agcgtgctcg
     1861 aagagggaaa ggggaagcgg tcgcgatcca atgcaacctc ccgggtgctg cgggtaagcc
     1921 tggggaatca ggccctcctg ctcacgggag agctcgagcg ggacggagac tcggtccgcc
     1981 tggcagagtc aaggccgatc catgccgcgg tgttgac
//
LOCUS       DBNGZC010000779         1489 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_949795, whole genome shotgun sequence.
ACCESSION   DBNGZC010000779 DBNGZC010000000
VERSION     DBNGZC010000779.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1489)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_949795"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..488
                     /locus_tag="AC16G0_13350"
     CDS             120..488
                     /locus_tag="AC16G0_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100638.1"
                     /translation="MTRHELLHGAALAPGTVVIAGISPTLALAGQSAQPKMDAQLIGK
                     LKPPELVLNSTRWPTKFGWKVIADEGYGIGTVGQSPAAPFFFRLKQAKREPEVAARPE
                     RAAARVARGHGECWPIWCGD"
     gene            461..1453
                     /locus_tag="AC16G0_13355"
     CDS             461..1453
                     /locus_tag="AC16G0_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011578924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5100639.1"
                     /translation="MLAYLVRRLILAILTVWAVSVLSFVIIQLPPGDYITSYVAQMSS
                     SGSFVSEQEAEALRKQYGLDQPQYIQYIRWMQMVLQGNYGMSLEYGRPVSEVIGDRLW
                     LTMVVSVAAIIFTWFVALPIGIYSAVRQYSLGDYVATFVGFIGLAIPSFMLALILMYL
                     GFTMFNANIGGLFSDQYAEAPWSLAKAWDLTKHLPLPGVILGLAGTAQLIRIMRANLL
                     DELGKPYVMTARARGLSESKLILKYPVRVALNPFASTIGYLLPYVVSGSIIVSLVLSL
                     PTVGPLLLKALIAQDMFLAGTIVLLLGIMTVIGTFLSDLALMWIDPRIRFEGKG"
BASE COUNT          249 a          480 c          464 g          296 t
ORIGIN      
        1 tgataccgtc caatcgcctg cttcgcaaat gctcaggccg gggccgtggg ttccgctccg
       61 gggccgtccg ccggacaaag cgcgaggaag ccccgatcag gtcgaagaat cgtgcgggcg
      121 tgacgcggca tgagcttctg catggagcgg cgctggcgcc ggggaccgtg gtgatcgcag
      181 gcatcagccc gaccctggcg ctcgccgggc aatcggccca gccgaagatg gacgcccagc
      241 tcatcggaaa gctgaaacct ccggaactcg ttctgaattc tacgaggtgg cccacgaaat
      301 tcggctggaa ggtcattgcg gacgaggggt acggcatcgg gacggtcggc caatccccag
      361 ccgccccttt tttcttcagg ctaaagcagg caaaacggga acctgaggta gccgcccggc
      421 ccgaacgggc ggccgcccgc gtcgcgagag ggcatggaga atgctggcct atttggtgcg
      481 gagactgatc ctcgctatct tgacggtgtg ggcggtgtct gtcctctctt ttgttatcat
      541 ccagcttcca cccggagact atatcacctc ctacgtcgcc cagatgtcgt cctccggcag
      601 cttcgtctcc gagcaagagg ccgaggccct gcgcaagcag tacggtctgg accagccgca
      661 gtacatccag tacatccgct ggatgcagat ggtcctgcaa gggaattacg gcatgtccct
      721 ggagtacggg cgtcccgtct cggaggtcat cggggatcgc ctgtggctga ccatggtggt
      781 ctccgtggcg gccatcatct tcacctggtt cgtggccctg cccatcggga tctactcggc
      841 ggtgaggcag tattcgctag gagactacgt cgccaccttc gtcgggttca tcggcttggc
      901 catccccagc ttcatgctgg ccttgatcct catgtacctc ggcttcacca tgttcaacgc
      961 caacatcggg gggctgtttt ccgaccagta tgcggaggct ccctggagct tggccaaggc
     1021 ctgggacctc acgaagcacc tgccgctgcc cggcgtgatc ttgggcctgg cgggcacggc
     1081 gcaactcatc cggatcatgc gggcaaatct gctggacgag ttgggcaagc cctacgtcat
     1141 gaccgcccgc gcccgaggcc tctcggagtc gaagctcatc ctcaagtacc ccgtgcgggt
     1201 ggccctcaac ccctttgcca gcaccatcgg ctacctcctt ccctatgtcg tctcggggag
     1261 catcatcgtg tctctggtgc tcagcctgcc gacggtcgga ccgctcctgc tcaaggccct
     1321 gatcgcccag gacatgtttc tggccgggac catcgtcctg ctcctgggga tcatgacggt
     1381 catcgggacg ttcctctcgg acctggcgtt gatgtggatc gatccccgca tccgattcga
     1441 gggcaaggga tgagcgagtc cgaggcggtt cgcgaggcct ccaccggaa
//
LOCUS       DBNGZC010000780         1952 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2220371, whole genome shotgun sequence.
ACCESSION   DBNGZC010000780 DBNGZC010000000
VERSION     DBNGZC010000780.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1952)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1952)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1952
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2220371"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..438)
                     /locus_tag="AC16G0_13360"
     CDS             complement(76..438)
                     /locus_tag="AC16G0_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5100640.1"
                     /translation="MDDTNCLRFFLEPTQTFHRQYEALRSVFVEDRPLSDVAHQFGYT
                     DETLRSLVRDFRARCRAGQASPFSPPRDAGDLLGITPPDHRPAQKRPPSPMYVNWTSL
                     QDAIAALGSLASSCSFRS"
     gene            753..>1952
                     /locus_tag="AC16G0_13365"
     CDS             753..>1952
                     /locus_tag="AC16G0_13365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100641.1"
                     /translation="MADGTAARSSRGHRTLCLPIPEGVYRQVVHDPGEFRRTLDDSFR
                     RIPEVFPAGFARGYELKDDRVSVKQGLPIRRILLKDGTAYSIRPSFLMPYMTARVQDV
                     EGPLFLRKFGVPFWALARVFGDDPMFWYRLECGLGRFSVVGTTVRRAGLPEHLLADEH
                     HQPLDGEKISIATTVGAGCCLGAEPAESAGADDLKAAYGVFKAEARDVAPQYAPKTLN
                     TDGWKPTQAAWKALFRRVIILQCFLHAWLKVRDRAKHLKGVFTEVSRRVWEAYHAPDR
                     RCFGQRLRSLRRWATGHIAGVVLEKVQDLCRTPRRWSIAYLHPGGHRTSNMLDRQMRG
                     MNRYFDHGQHLHGSLGACRLHCRALGIAVELHAMAPGYDPGERRLALSCRTPQPAPLP
                     RVLAAKPA"
BASE COUNT          338 a          595 c          687 g          332 t
ORIGIN      
        1 gggcgctggt ggcgggcacc atgtgagagc ccggatagtt cgcctgctgc accaactgat
       61 cgaagcacaa tcgggtcaag agcggaagga acaagaagac gccagcgacc cgagtgcggc
      121 aatggcgtcc tggagtgagg tccagttgac gtacatcggc gatggcgggc gtttctgggc
      181 cggtcggtgg tccggtggcg tgatcccgag caggtcgccc gcgtcgcggg gtggcgaaaa
      241 gggggaagcc tggcctgccc gacaccgggc gcggaagtca cggacgagcg agcgcagcgt
      301 ctcgtccgtg tagccgaact ggtgggcgac atcgctcaag gggcggtcct caacaaagac
      361 gctacggagg gcctcgtatt ggcggtggaa ggtctgggtg ggttccagga agaagcgaag
      421 gcagttggtg tcatccacgc ccggagctcc gagatgaggg tcgattctcg tcgagtggcg
      481 ggcctgatgg tatgccttcg gcgtgagggc tccaaggggt gagatcgagt ccgtggcctc
      541 gtgcacggcc gattcgaggc ttgtgccggg cggaggacac agcctcaatg acaagtgaat
      601 ccgggttgaa acgtaatgaa ctggtcgtca gtcccggtag aaaggactgg agttttgctg
      661 accgtcacgg attttggggg gcagggttcc caggctgacg tgacaagaca aaacggccat
      721 gatccacggt ggcaccggga aggaggtgga tcatggccga tgggaccgcg gcacggtcct
      781 cacgggggca tcgtacgctc tgcttgccca tcccggaagg agtctatcgg caggtcgtcc
      841 acgatcccgg cgagttccgg cgtacgctcg acgactcgtt ccggcggata cccgaggtgt
      901 tcccggcggg cttcgcccgg ggctacgaat tgaaggacga tcgtgtctcg gtcaagcaag
      961 ggctgccgat ccgacgcatc ctcctgaaag acgggacggc ctacagcatc cggccgtcgt
     1021 tcctcatgcc ctacatgacc gcgcgtgttc aagacgtcga aggccccttg ttcctccgca
     1081 agttcggcgt gcctttctgg gccttggccc gcgtcttcgg cgacgacccg atgttctggt
     1141 atcgcctgga atgcggcctc ggacgcttca gtgtcgtggg gacgaccgtc cgtcgggcag
     1201 ggttgcccga gcacctgttg gccgatgagc atcaccaacc gctcgatggc gagaagatct
     1261 ccattgccac gactgtcggc gcggggtgct gtctgggggc cgagccggcc gagtcggcgg
     1321 gggccgacga cctgaaggcc gcctacgggg tcttcaaggc cgaggcccgc gacgtcgccc
     1381 cacagtacgc tcccaagacg ctcaacaccg acggctggaa gccgacgcaa gcggcctgga
     1441 aggccctgtt ccggcgtgtg atcatcctcc agtgcttcct gcacgcctgg ctcaaggtcc
     1501 gtgaccgagc caagcacctg aagggggtgt tcacagaggt ctcccgccgg gtctgggagg
     1561 cgtatcacgc gccggaccgc cgatgcttcg gccaacggct ccggtcgctg cggcgctggg
     1621 cgaccgggca catcgcgggg gttgtcctgg agaaggtcca ggatctgtgt cgcacgccgc
     1681 ggagatggtc gatcgcgtac ctgcaccccg gcgggcaccg caccagcaac atgctcgacc
     1741 gccagatgcg ggggatgaac cggtacttcg accacggcca gcatctgcac ggctcgctcg
     1801 gggcctgccg gctgcattgc cgcgccctgg gcattgctgt ggaacttcac gccatggcac
     1861 ccggctacga cccgggagaa cggcggctgg cgttgtcctg ccgaacgcct caaccggcac
     1921 cgctaccacg agtgttggct gcaaaacctg ct
//
LOCUS       DBNGZC010000781         1510 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2123351, whole genome shotgun sequence.
ACCESSION   DBNGZC010000781 DBNGZC010000000
VERSION     DBNGZC010000781.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2123351"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="AC16G0_13370"
     CDS             <1..477
                     /locus_tag="AC16G0_13370"
                     /inference="COORDINATES: protein motif:HMM:NF024989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR5100642.1"
                     /translation="GASRLVMTGLQVDAERLKVAESLGAHTVMADATDPGEAVRDLTR
                     GLGADVVFECAGHPSGVPQALTLVRKGGKIGVLGQGHHDSAFNTTILSYREVELIGVR
                     AYDPRTWYRSHDVLASRQFPLETLITHRLPLQEAARGIELMQSKQGLKIMFTPTWA"
     gene            502..1314
                     /locus_tag="AC16G0_13375"
     CDS             502..1314
                     /locus_tag="AC16G0_13375"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR5100643.1"
                     /translation="MPLTVGACTYSWLWDRPLQDAVHRIAEMGFKYFELMSTPPHCWP
                     RDYSAADRTGFRRLYESLGLRLTSVNPTFLDLNMASPNPGIREESIRQLRETVQLAHD
                     IGASIVVAPGGRKHLLIAPDQSYVWGLVKGALESLLPDCERLGVFFGLENGYNVTHTA
                     ELMVQMCRDLSHPNLKLVYDVANATMVESPLDGLGLVAMDMALLHVSDTDDKVWGHDR
                     IGTGVVDFAAVTEKVKKIGYAGPTIMEIVDRRTPDESNRLSLQRMRALGWTT"
     gene            1302..>1510
                     /locus_tag="AC16G0_13380"
     CDS             1302..>1510
                     /locus_tag="AC16G0_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100644.1"
                     /translation="MDDVVHFELFQNVSNREGDARRKAGFAEQARWLANQGAARGRDQ
                     EIPMAEVDHGACIDSNRERGLSVLSG"
BASE COUNT          287 a          444 c          514 g          265 t
ORIGIN      
        1 ggagcctcgc ggctcgtcat gaccgggctc caggtggacg ccgagcggct gaaggtcgcg
       61 gagtcgttgg gggcccacac cgtcatggca gatgcaaccg acccagggga ggcggtccgg
      121 gacttgaccc gcggtctggg cgcggatgtg gtcttcgagt gcgccggcca tcccagcggt
      181 gtgccgcagg cgctcaccct ggtgcgcaag ggcgggaaga tcggcgtcct gggtcagggg
      241 catcacgact cggccttcaa caccaccatc ctctcctacc gggaggtgga gctgatcgga
      301 gtccgcgcct acgatccgag gacctggtac cgcagtcatg acgtcctggc cagccgccag
      361 ttccccctgg aaaccctcat cacccaccgc ctgcccttgc aggaggcggc ccgtgggatc
      421 gagctaatgc agagcaagca aggtctcaag atcatgttca cgccgacctg ggcctgatcg
      481 ctgtcggaag gagaatcagc catgccactg accgttggtg cctgcacgta ctcatggttg
      541 tgggaccgtc cgctgcagga tgcggtacac cggatcgccg agatggggtt caagtatttc
      601 gagttgatgt ccacgccccc ccactgctgg ccgcgcgatt attcggcggc tgacaggaca
      661 gggttccgga gattatacga gtccctgggg ctgcggctca cgtccgtaaa cccgacattc
      721 ctggacctca acatggcaag cccgaaccca gggatccgcg aggagtcgat ccggcagctt
      781 cgggaaaccg tccaactcgc ccatgacatc ggggcgagca tcgtggtggc ccccggcggc
      841 cgaaagcatc tgctcatcgc ccccgatcaa tcctatgtct ggggcctggt gaagggggcg
      901 ctggaatcgc tcctcccgga ctgcgagcgg ctgggggtgt tctttggcct ggagaacggc
      961 tacaacgtga cgcacaccgc ggagttgatg gtccagatgt gccgcgacct gtcgcacccg
     1021 aatttgaagc tggtgtatga cgtggccaat gcaaccatgg tggaatctcc cctggacggc
     1081 ctagggctgg tggcaatgga catggcgctg ctccacgtgt cggataccga cgacaaggta
     1141 tgggggcacg atcggatcgg caccggggtt gttgacttcg ccgccgtgac cgagaaggtg
     1201 aagaagattg gttatgccgg tccaaccatc atggagatcg tggaccggcg cacacccgac
     1261 gagagcaatc ggctcagctt gcagcgtatg cgggcgctgg ggtggacgac gtagttcatt
     1321 tcgagctgtt ccagaacgtt tcgaaccggg agggagacgc gaggagaaag gcaggattcg
     1381 ccgagcaggc gaggtggctg gcgaatcagg gcgcagccag gggtcgagat caggagatcc
     1441 ctatggcaga ggtagatcac ggggcgtgca tcgactcaaa ccgagagaga ggtctgtcgg
     1501 tactctctgg
//
LOCUS       DBNGZC010000782         1616 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_68921, whole genome shotgun sequence.
ACCESSION   DBNGZC010000782 DBNGZC010000000
VERSION     DBNGZC010000782.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_68921"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            488..>1616
                     /locus_tag="AC16G0_13385"
     CDS             488..>1616
                     /locus_tag="AC16G0_13385"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5100645.1"
                     /translation="MSSMNRISRRKFLKAVGGVAGVAAGAGLLPRKAHSQTRTITIWH
                     TEASDIAVKAVQRVCDRFEQLHPGVKAIQQGIGWDSLGPKLYTGLAAGSPPDISQLNC
                     YHFSSFHSKGSIIPLDDVYAAIGLNDIVEVVRDMSLVDGHRWGISHEIGCPLIMIRKD
                     IAQQAGYTVPDDVTQPMFKGWKEQLEYLKAVTKPDKRQWGMSLPGTGYFLQEHVGRWV
                     SSNGGGYYDSKWNPIFHTEPFIQTLEFVKALSDSKVVPPDWLSQSWLGMIVEFSTGIT
                     TMIDHGYGRIAGSIEKYAPERANEQSYYPVWRPLGPNGSKPYTDLDAELWVVFKQSKN
                     QDLAKEWLKLFYTRDLYLNYIRQYPVHMFPITKSLRNDPEYK"
BASE COUNT          326 a          502 c          518 g          270 t
ORIGIN      
        1 cgcaaccacc gcacccccga caggcgtggg ctgccaggac agtgacttgc ggagaacggc
       61 tggtcccgcc ctcgtcccct gtatgccgat gtccgcagga accggtgctc tcgtcatcct
      121 tctgccattc cccgcgcggg ttcacccgac aatcgaacgc cttggggcct gcggacgtcg
      181 gttgccgatg gtctcggcac cgaaagccta gtccaagtcg gcgactggac gagcgggaat
      241 aaagggaggg cgcgggtctg cttttccctt gacacgcccg caggttcgag gatagcgtgt
      301 cgccaccctg aaaccggttt caacaccgtc agggtaatgc tggcattgac aggtctggag
      361 gtccgggcct tctgaagata agtccccggg cttcccgaca tgttcccggt ccagggcggc
      421 acgccgcggg actggagggc gggcggggtg gcccgcgtat ttgtcgtgga gaaaaggagg
      481 cgcgaccatg tccagcatga accgcatcag ccgcaggaag ttcctcaagg ccgtgggtgg
      541 agtcgcggga gtcgcggcag gagccgggct ccttccgcgc aaggcgcatt cccaaacccg
      601 caccataacg atctggcaca ccgaggcctc ggacatcgcg gtgaaggctg ttcagcgggt
      661 gtgcgatcgg ttcgagcaat tgcaccctgg ggtgaaggcg atccagcagg gaatcggatg
      721 ggacagcctg ggccccaagt tgtacaccgg gctggcggcc ggcagcccac ccgacatctc
      781 ccagctcaac tgctaccact tcagctcctt ccacagcaag ggctccatca ttcccctgga
      841 cgacgtgtac gcggccatcg gattgaacga catcgtggag gtggtgcgag acatgtcgct
      901 ggtggacggc caccgctggg ggatctcgca cgagattggc tgcccgctca tcatgattcg
      961 caaagacatc gcgcagcagg ccggatacac ggtgcccgac gacgtcaccc agccaatgtt
     1021 caaggggtgg aaggagcagc tcgaatacct gaaggccgtg accaagccgg acaagcgcca
     1081 gtgggggatg agcctgcccg ggacggggta ctttctccag gagcatgtgg ggcgctgggt
     1141 cagctccaac ggcggcgggt actacgacag caagtggaac ccgatcttcc ataccgagcc
     1201 gttcatccag acgttggagt tcgtgaaggc gctgtccgac agcaaggtgg tgccgccaga
     1261 ctggctgagc cagtcatggc tggggatgat cgtcgaattc tccacgggca tcaccaccat
     1321 gatcgatcac gggtacggcc gaatcgccgg ctccatcgag aagtacgccc cggagagggc
     1381 caacgaacag tcctactacc cggtgtggcg gccgctgggc ccgaacggca gcaagcccta
     1441 tacagacctg gacgccgagc tgtgggtggt gttcaagcag tcgaagaacc aggacctcgc
     1501 caaggaatgg ctgaagctct tctacacgcg ggacctctac ctcaactaca tccggcagta
     1561 cccggttcac atgttcccca tcaccaagtc gctgcgaaac gatccagagt acaagg
//
LOCUS       DBNGZC010000783         2321 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_69044, whole genome shotgun sequence.
ACCESSION   DBNGZC010000783 DBNGZC010000000
VERSION     DBNGZC010000783.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_69044"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="AC16G0_13390"
     CDS             complement(<1..672)
                     /locus_tag="AC16G0_13390"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR5100646.1"
                     /translation="MTVLAIDIGGTKLAAGVVDRDGRILARGEVPTLAAEGPARVLER
                     IVGLGQALLGRPEVSRGAVHRIGIGCAGPVDRLAGLVLNPPNLPGWTRVALASHLQQA
                     FGLPAVLENDANAAALGEFRYGAGKGARSLVYLTVSTGIGGGIILDGKIWHGLNDAAG
                     EIGHMTLIPDGPLCGCGNHGCLEAIASGPSIARRAREALTDGRPTRLRGMPGLTSADV
                     VRLAQE"
     gene            complement(690..1937)
                     /locus_tag="AC16G0_13395"
     CDS             complement(690..1937)
                     /locus_tag="AC16G0_13395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5100647.1"
                     /translation="MAARLFYGWIVASVVCLMWAVSIGPRQSFSIFLLAFTEDFGWSR
                     SATTAAFSIHMTFYALGGWILGMLVDRLGPRRVIAWSTVAWVLVLILCSQVQSLWQFY
                     LIYGVVGGVATGGLAYVPINSLLSRWFIRYRGSATGITQAGVPMGTAAFGPLAQFGIV
                     LIGWRMTHVAFGFLVAAISLPLVLCFLRDDPREMGLTPDGTPAAAKNPDGSEEPAARS
                     RAFSGAGLPSSYWSVFGANVLRGMTMYAVLVHQVAYLVDVGYTKMAAATYYSLASILA
                     IPAGLAAGVLSDRLGRQRAYAGIAGLYVVGYVCLLLVRSPSQTFLLYAFVLAIGMATG
                     GGPPVFAAFLTDRLQGSRVGFLLGLQNIGFGLGATFGPFLAGASFDLLGSYSPAFLLM
                     AAAMLVSSAIVSGSARRSTPAPG"
     gene            complement(2186..2317)
                     /locus_tag="AC16G0_13400"
     CDS             complement(2186..2317)
                     /locus_tag="AC16G0_13400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c(7)-type cytochrome triheme domain-containing
                     protein"
                     /protein_id="HIR5100648.1"
                     /translation="MQKGADKLNMNDMGEGKACGACHNGQKAFGVMDGDRCATCHKS"
BASE COUNT          463 a          773 c          749 g          336 t
ORIGIN      
        1 ctcttgcgcc aggcggacga cgtcggctga tgtgagaccc ggcatccctc gtagacgggt
       61 cggccggccg tcggtcaggg cctcccgcgc ccggcgtgca atggaaggac cggaggcgat
      121 cgcctccagg cacccgtggt tgccacagcc gcagagggga ccgtcgggaa tcagggtcat
      181 gtggccgatc tcgccggccg cgtcgttcag gccgtgccag atcttcccat ccaggatgat
      241 ccctccgccg atacccgtgc tcaccgtgag gtacaccagg ctccgcgccc ccttgcccgc
      301 gccgtagcgg aactcgccca aggccgcggc attggcgtcg ttctcgagaa cggccggaag
      361 gccgaacgcc tgctgaaggt gactcgccag ggcgacccgc gtccagcccg gaagattggg
      421 cggattgagg accaacccgg ccaggcgatc caccggcccg gcgcagccga tcccgatccg
      481 atggaccgca cctctggaga cctccggcct gccgaggagg gcttgcccca ggccgacgat
      541 tcgctcaagg acccgagcag gtccttccgc agccagcgtt ggcacctctc cgcgggccag
      601 gatcctgcca tccctatcca cgacccctgc cgccagcttc gtcccaccga tatctattgc
      661 cagaacggtc atgggcatgc ctcgaatcct taaccagggg caggggttga gcggcgcgcc
      721 gatcccgaga cgattgctga agagaccagc atggcagcgg ccatcaagag gaacgcgggg
      781 ctgtaactgc caaggaggtc aaaggacgcg ccggcgagga aaggaccgaa tgtcgcgccc
      841 aatccgaagc cgatgttctg caggccgagg aggaacccca cgcgcgaacc ctggagccgg
      901 tcggtgagga aggcggcgaa gaccgggggt cccccgcccg ttgccatgcc gatcgcgagg
      961 acgaaggcat acaacaagaa cgtctgcgac gggctacgaa caaggaggag gcacacgtat
     1021 ccgaccacgt acaggccggc aatcccggcg taggcccgct ggcgacccag gcgatccgag
     1081 agaacgccgg cggcgagccc cgcggggatc gccaggatcg aggccaagga gtaataggtg
     1141 gcggccgcca tcttggtgta ccccacgtct accaggtagg cgacctgatg gacgaggacc
     1201 gcatacatgg tcatgccccg gaggacgttg gcgccgaaca ccgaccagta gctgctgggc
     1261 aggcccgccc ccgagaacgc ccggcttcga gcggcgggct cctcggaccc atccgggttc
     1321 ttcgctgccg caggggtacc gtccggtgtg agccccatct cccgtgggtc gtcccgaaga
     1381 aagcagagaa ccaacggcaa ggagatcgcg gcaaccagga acccgaaggc aacgtgggtc
     1441 atccgccatc ctatgaggac aatgccgaac tgggcgagcg gtccgaaggc ggctgtgccc
     1501 atggggacgc ctgcctgggt gatccccgta gccgagcctc gataccggat gaaccaacgg
     1561 gagaggagag aattgatggg cacgtacgcc agaccacccg tggccacgcc acccaccacc
     1621 ccgtagatga ggtagaactg ccagaggctc tggacctgac tgcacaggat caacacgagg
     1681 acccatgcca cggtgctcca cgcgatcacg cgccggggcc ccaggcgatc taccagcatg
     1741 ccgaggatcc agccgcccaa ggcatagaag gtcatgtgaa tggagaaggc cgccgtcgtg
     1801 gcgctgcggc tccaaccgaa gtcttccgtg aaggcaagca ggaagatgga aaaggattgc
     1861 ctggggccga tggagacggc ccacatgaga cacacaacgc tggcaacaat ccatccgtag
     1921 aacaggcgtg cagccattcc cgtggtaact ccttccgatg ggacagagcc tcacatccga
     1981 gacagccagg ggaataaggt tggcactgtg tgccggacac gctggaagtc tgtccattcc
     2041 aagaaggcga tgtttcggga atggcccagg cgggttggtg tcgatcgcga caagcatccc
     2101 ctggcccgtc ggcgccaggc ctccgtggtc cgttcacgac gcccgtaggc gatgcgccaa
     2161 tggctacggg ctcccctcgc ctggctcagg acttatgaca cgtggcgcac ctgtcgccat
     2221 ccatcacacc gaaggccttt tgaccattgt ggcaggcgcc gcaggccttc ccctccccca
     2281 tgtcgttcat gttgagcttg tcggccccct tctgcatttt g
//
LOCUS       DBNGZC010000784         7165 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_459082, whole genome shotgun sequence.
ACCESSION   DBNGZC010000784 DBNGZC010000000
VERSION     DBNGZC010000784.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7165)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7165)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7165
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_459082"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="AC16G0_13405"
     CDS             <1..1335
                     /locus_tag="AC16G0_13405"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR5100649.1"
                     /translation="ELADRTRQLEAIRAVTQEITRELDLTTLLGLIARRATELVGATS
                     GTVFLWDEATQVLVPQAWHGPGTWKGDSRLRLGEGVAGTVAKQRVGLVVNDYRAPSDA
                     LPVFPENTKATAILGEPLLYRDRLLGVITVDDEDTGVPFSQQDRECFALFVAQAAIAI
                     ENAQLYEATRQHAAELEARVEERTRALADASRHKSEFLASMSHELRTPLNSILVFAQI
                     LLEQTGEVLSSKQTRFLSNIYNSGQHLLQLINDILDLSKVEAGKIVLQPEALPLEETL
                     EDILVIARGLANKKSQTVRMEIEAGLPSLYADPVRFKQILFNLLSNAVKFTPEGGKIT
                     VRARRLPGMVDGKLQAADRSETSEIAGLYSGMLEIAVTDTGIGIKADDFPQLFQEFVQ
                     LEGAKRQEGTGLGLALTKRLVELHGGRIWAESKGEGWGSTFVVVFPCGGVDG"
     gene            1433..1819
                     /locus_tag="AC16G0_13410"
     CDS             1433..1819
                     /locus_tag="AC16G0_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5100650.1"
                     /translation="MTRRVLVVEDNLVNLELMAAILEEEGYEVLSAETAVAGLRMAEG
                     EHPDLILMDIHLPGMTGYEATRKLKADPRTAAIPVVAVTAQAMQGEDAKARAAGCDAY
                     LTKPLDTQGFRGTLRRFLGDRGTPPT"
     gene            2120..2905
                     /gene="amrB"
                     /locus_tag="AC16G0_13415"
     CDS             2120..2905
                     /gene="amrB"
                     /locus_tag="AC16G0_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HIR5100651.1"
                     /translation="MAGTFYAGTRERLRLQVEDLLPRGVVPESAIGVVVPHAGYLYSG
                     RVAGAVYARVEFPATCVILGPNHTGLGAGAAIMTDGRWETPLGEVPIDHDLATAIRGN
                     AALLEEDSRGHLREHSIEVQLPFLQYCGRPFQFVPICLFSAEYAACQNVGQAVAAAVG
                     QTGKRVLLIASTDMSHYVSRAEAAAKDRKAIDAILALDPEGLHRVVRREGISMCGVHP
                     TVAMLIAAKALGATQADLVMYTDSGAVTRTTEEVVAYAGLIVR"
     gene            3135..3476
                     /locus_tag="AC16G0_13420"
     CDS             3135..3476
                     /locus_tag="AC16G0_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007333249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HIR5100652.1"
                     /translation="MTYIVAEPCIGTKDHACVDVCPVECFYEGEDMLYIHPEECIDCG
                     ACVPECPVAAIFEESDTPEQWKSYIAKNADIFKENPNIPKAPTREKWEELKKEPTSEA
                     AKYYQKYPPKS"
     gene            complement(3750..4271)
                     /locus_tag="AC16G0_13425"
                     /pseudo
     CDS             complement(3750..4271)
                     /locus_tag="AC16G0_13425"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
     gene            complement(4312..5094)
                     /locus_tag="AC16G0_13430"
     CDS             complement(4312..5094)
                     /locus_tag="AC16G0_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100653.1"
                     /translation="MISLQQLNALPPPERDRCYLSLVPPALCQRFGLDAAAFTDPAGR
                     PAARVEVSGKPPAVQISITHTSDALDPVFFLRLEETRYGNLQIRFIILSDPQGPRFGV
                     DLDDAGNMTGLGVRSRNTREEERAMRAGLAPGQIRPGIRLLGPALQRIETFAARLGKT
                     ALILEAFFYHNALLYERHGFGYLAGQDRMRLIHRGFLPGGVLEMKLDGSSPFRSPGAG
                     GTIRGRSWAIQDSILPQRWWPPVMYRPLGVRLDICTAPDVPF"
     gene            complement(5116..5703)
                     /locus_tag="AC16G0_13435"
     CDS             complement(5116..5703)
                     /locus_tag="AC16G0_13435"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR5100654.1"
                     /translation="MATAVAGLQAVDAWIFHLINRSFQNSLFDLLMPILSTKEYLILP
                     ALGVLLFLALRGGRREWLLLLVGVAAVALSDQWANLIKAAFHRTRPCHVFPDVHLLAG
                     CTRSFSLPSNHASNMFAIAAVGWLTLRRWRWALPLLAVAVAYSRIYLGVHYPSDVIAG
                     ALWGVLAGWACTRIAAYAIPGWSQTRPKPAPPSSA"
     gene            complement(5712..6686)
                     /locus_tag="AC16G0_13440"
     CDS             complement(5712..6686)
                     /locus_tag="AC16G0_13440"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR5100655.1"
                     /translation="MKPQPPRPGWLPILGKLLVSGCLLGLLFWRVDRSAFLRSVEALP
                     VSVFVGCVGLYTLGYVISTLRWQRLLAAEGIHLPLWRLILVYFEAAFFNLFLPTLIGG
                     DIVRGYTIYRLTRGHDASIASILVDRLSGFAAMVVIALVALAVAYRRLQDPQVTGMVL
                     AVAGAFVLAMTLLLNDRMKTGATGILRLVGLVRFQAKIQGMVEAIHRYRRHHQALGQA
                     LVLSGMLQALIIVTYYLIGTSLKLGVPLGYFFLYVPLITVMAMLPISVAGLGVREGGV
                     VYFFAKVGVEPAVALSMSLVWFSLSLAVSSLGGLAFLLDTHAAKRLEG"
     gene            complement(6683..>7165)
                     /locus_tag="AC16G0_13445"
     CDS             complement(6683..>7165)
                     /locus_tag="AC16G0_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5100656.1"
                     /translation="RREVVEHLHLYGEMHRFIPAIASWMGISLTEVETHHRPRRFGRS
                     KYGIARTLRVILDLITVKFLLSFATKPIQVFGLLGLLASGAGILIGLYLTVLKLAFGA
                     QIGGRPLLFLGILLILVGVQLTVMGLLGEMLVRVYHESQRKPIYMVKRILGHPGRENP
                     "
BASE COUNT         1439 a         2133 c         2414 g         1179 t
ORIGIN      
        1 gaactggccg accgcaccag acagcttgag gccatccgcg cggtcaccca ggaaatcacc
       61 cgtgagctgg acctgacgac gctgctgggc ctcatagccc ggcgcgcgac cgaactggta
      121 ggcgccacgt cgggaacggt gtttctgtgg gatgaggcga cccaagtcct cgtcccacaa
      181 gcctggcacg gccccgggac gtggaaaggc gacagccggc ttcgacttgg ggaaggcgtg
      241 gccggtaccg tcgcgaagca gcgggttggc ctggtcgtca acgactaccg ggcgccgtcg
      301 gatgcgctcc cggtcttccc ggagaacacg aaggcgaccg ccatcctggg tgaaccactc
      361 ctttaccgag atcggctcct gggggtcatc acggtggatg acgaagacac gggcgtgccc
      421 tttagccagc aggatcggga gtgcttcgcg ctcttcgtcg cgcaagccgc cattgcaata
      481 gagaatgccc aactctacga agccacccgt cagcacgccg ccgagctcga ggcccgggtg
      541 gaagagcgga cgcgggcact ggcagacgcc tcccgccata aatccgaatt tctggccagc
      601 atgtcccacg agcttcggac cccgctcaac tccattctgg tcttcgctca gatcctcctc
      661 gagcagacgg gggaagtgct ctcatcaaag cagactcggt tcctttcaaa catttataac
      721 agcggccaac acctcctgca gctcatcaat gacatcctgg atttgagcaa ggtcgaggcg
      781 gggaagatcg tcctccagcc cgaggccctg ccgctcgagg agaccctgga ggatatcctg
      841 gtcatcgccc gcggtctggc gaacaagaag agccagaccg tccggatgga gatcgaggcg
      901 gggttgccct cactttacgc cgacccggtc cggttcaagc agatcctgtt caatcttctg
      961 agcaatgcgg tgaagttcac gcccgagggc gggaagatca cggtccgggc gcggcggctc
     1021 cccgggatgg tcgatggcaa gttgcaggct gcagatcgct cggagacatc tgaaatcgca
     1081 ggtctgtact ctgggatgct cgagattgcc gtgaccgaca ccggcatcgg gatcaaggcc
     1141 gatgattttc ctcagctgtt ccaggagttc gtgcagcttg agggggccaa gcgtcaagag
     1201 gggaccggcc tcggcctcgc cttgaccaag cgacttgtgg aacttcacgg gggacggatc
     1261 tgggccgaat ccaagggaga ggggtggggt agcacgttcg tcgtggtctt cccctgtggg
     1321 ggcgtcgacg ggtgaaaccc agacggggaa aatgagaatg gattcccggg ggggtcgccc
     1381 ctgaagggca agggactagc gttgctcatt gaatatccct ggaggtggtc tggtgactcg
     1441 tcgggtactc gtggtggagg acaacctggt caacctggag ctgatggccg ccatcctgga
     1501 ggaggaaggg tacgaggtgc tcagcgccga gacggccgta gccggcctcc gcatggccga
     1561 aggcgagcac cccgatctca tcttgatgga cattcacctc cccggcatga ctggctacga
     1621 ggccacgcgg aagctcaagg cggacccacg caccgcggcg atcccggtgg tggccgtcac
     1681 cgcccaggcc atgcagggcg aggatgccaa ggcgcgggcg gccggctgcg atgcctacct
     1741 cacaaagccg ttggacaccc agggcttccg ggggacgctg cggcgattcc tcggcgatcg
     1801 ggggacgcca cccacgtagg cgacgacggg tcggggcggc gtcttttcgc ctgcgaggcc
     1861 gagattcaac cgcgtggggc catcggatgg agggtcggcg ttgacaaact tgggggatca
     1921 tgccagattt ccatgcggcg ataagctggg gcctcgattc gtgaggtgca gaatggtccc
     1981 ggtcgggtcc tctccgcact cgatgagacg cgcgaggtag cgcggagatc tcaagacgtc
     2041 ttcgaggggt gcatttcctc gaagacgttt tttctttccg tcgtgaggga aggggacggc
     2101 cctgatccga cgagcggcgg tggcagggac cttttacgcg gggacgcgcg agcggttgcg
     2161 gctgcaagtg gaggacttgc tcccgagggg ggtggtgccg gaatcggcca tcggcgtggt
     2221 cgtgccgcat gcagggtacc tgtactccgg acgggtggcg ggggcggtct atgcgcgcgt
     2281 agaattcccc gcgacctgcg tgatcctcgg ccccaaccac accggcctcg gggcgggggc
     2341 ggcaatcatg acggacggac gctgggagac accgttgggg gaggtcccca tcgaccatga
     2401 tctagcgacg gcgatccgcg ggaacgcagc cctcctggag gaggactccc gtgggcatct
     2461 ccgcgaacac tccatcgagg tgcagctccc cttcctgcaa tattgcgggc gccccttcca
     2521 attcgtcccc atctgcctgt tcagcgcgga gtacgctgcc tgccagaacg tggggcaggc
     2581 ggtcgcggcg gccgtcgggc agacggggaa gcgcgtgctc ttgatcgcca gcacggacat
     2641 gagtcactac gtctcccgtg cggaggcggc ggcgaaggat cggaaggcca ttgatgcgat
     2701 cctggccctg gacccggagg ggctccatcg ggtggtgcgt cgggagggta tcagcatgtg
     2761 cggcgttcat ccgaccgtgg ccatgctgat cgcggcgaag gcgctcggag cgacccaagc
     2821 tgacctcgtg atgtacacgg attctggcgc agttactcgg accacggagg aggtcgtggc
     2881 ctacgccggc ctgattgtcc gttgaagaaa gttcaggctg caaattgacg atcgccgatg
     2941 ggagtgctca actggaaatc agccctccga aatctctgac cagggctaca accagttgac
     3001 gggtttgccc tgaccgcgta gaattctttt cgcgaggctg ggagccgtca ggtcgtctgg
     3061 ttgagcgaca gatatcgcgc aacaacgtgc ggaggagaag cattcgtggg ggtcgtgaat
     3121 aggaggaggg cacgatgacc tatatcgtgg cagagccgtg catcgggacg aaggatcacg
     3181 cctgcgtaga tgtctgtccc gtcgagtgct tttacgaagg cgaggacatg ctctacattc
     3241 acccggagga gtgtatagac tgtggggcat gtgtgcctga atgtccggtg gcggccatct
     3301 ttgaagaaag cgacactcct gagcagtgga agagctacat tgccaagaac gcagacatct
     3361 tcaaggagaa ccccaacatt cccaaggcgc ccacgcgaga gaaatgggaa gagctgaaga
     3421 aagaaccgac cagcgaggcg gccaagtatt atcagaagta tccgccgaag tcctgaccgg
     3481 cccaatttct ctaccagctc acgaagaaac ttcacgccca ggcgactgaa agagtcgcct
     3541 gggcgttttg ttctcgggga agagtcggct caggaacaag tagactagct agaatccgta
     3601 tctcaccgga ccggaaacag attatgtgat ctttcgtacc tgagatctga aggaagggca
     3661 gcaagggtaa aggtatgtgt aagacttcga tgaggataga gaaccctcga ccgcagtgag
     3721 gcgctggtgc gatgggatgt atgaaccagt caagctctga ggcgccgggt ttcaagtgag
     3781 gttgggggat tctcttgtgg ccagtccacc ggtcgcggct gcgccggcag cgcccggatg
     3841 agcaagacgg gcttgaggct accgcggacg accttctccg cgatgctgcc cataaggaac
     3901 cgatccagtc cactccgccc gtgggtggcc atggcaatca gatcaaaccc cttggaccct
     3961 gcatagccga ggatcgaatc ggccacgtcg ccctcaaggg tgtcgcagtg agcggtgaca
     4021 ccctgatcct tcagcccccg ctggatcctc tccaggtaca tcatggcctc ggcgttcgcg
     4081 gccgttctgc gctcggccac gacctccggc aaggtttcga cgtaggcctt cccggaacct
     4141 cacatggcgc gctggacgac ggtgaccagg gttacttcgg ccctggtggc cttggccaca
     4201 gcaatggcat gcggcaggac catctcggcg agctgtgaac cgtcgagggg caccaggatc
     4261 ctctggtaca taccaccacc tcctctcccg tagtatcgaa gagcccagcg atcagaacgg
     4321 cacgtcgggc gccgtgcaga tgtcgaggcg gacccctaag gggcggtaca tcaccggtgg
     4381 ccaccagcgc tgcggcagaa ttgaatcctg aatggcccag cttcgaccac ggatggtccc
     4441 gccagcccct ggcgatcgaa aggggctgct cccgtcgagt ttcatctcca ggacgccgcc
     4501 aggcaggaat ccccggtgaa tcaaccgcat gcgatcctgg cctgccagat agccgaatcc
     4561 gtgccgttcg tagaggaggg cgttgtgata aaagaaagcc tccaggatga gcgcggtctt
     4621 tccgaggcgt gccgcgaagg tctcgatccg ctgcagtgca ggtccaagca gtctgatgcc
     4681 tgggcggatc tgccccgggg cgagtccggc gcgcatggcg cgctcctctt cccgtgtgtt
     4741 gcgggagcgg acacccaggc ccgtcatgtt gcctgcgtcg tccagatcga caccgaagcg
     4801 cggaccctga ggatcgctga ggatgatgaa ccggatctgc agattgccgt accgcgtttc
     4861 ttcgagccgg aggaagaaga cggggtcaag ggcatcggaa gtgtgagtga tgctgatctg
     4921 gaccgcgggg ggcttcccgg agacctccac ccgggcagcg ggtcgcccgg cgggatcggt
     4981 gaaggccgcc gcgtccagcc cgaaccgctg gcacaatgct ggaggcacca gggagaggta
     5041 gcagcgatcc ctttcaggcg ggggcaacgc attgagttgc tgcaggctga tcatgccgcg
     5101 tggtctcgcc ggacttcagg ccgagcttgg cggagccggc ttgggccgcg tctggctcca
     5161 cccggggatt gcataggctg caatccgggt gcacgcccag ccggcgagca ccccccacag
     5221 cgcaccggcg atgacgtccg acggatagtg gacgcccagg tagatccggg aatatgccac
     5281 ggcaacggcc aggaggggga gcgcccagcg ccatcgacgg agcgtgagcc atcccacggc
     5341 cgcaatggca aacatgttgc tggcgtgatt ggagggcagc gagaaggagc gagtgcaccc
     5401 ggcgaggaga tgcacatcgg ggaacacgtg acacggccgc gtgcggtgga aggcggcctt
     5461 gatcaggttg gcccattggt cgctgagggc cacggcggcc accccgacga gcagcagcag
     5521 ccactcccgc cggcctcccc gaagcgccag gaagagcagg accccaaggg cgggaaggat
     5581 gagatactcc ttggtgctga gaatcggcat caagaggtcg aacagcgaat tctggaagga
     5641 gcgattgatc aggtggaaga tccacgcgtc cacggcttgc agtcctgcca cggcagtcgc
     5701 catcggccga ctcagccctc gagccgctta gcagcgtgtg tgtcaaggag gaaggccaga
     5761 cccccgaggc tgctgacggc taggctgagg gagaaccaca cgagcgacat gctaagcgcg
     5821 acggccggct cgaccccgac cttggcgaag aagtagacga cgccaccctc gcgcacgccg
     5881 agccccgcca ccgatatggg gagcatggcc atcacggtga tcagggggac atacaggaag
     5941 aaatacccca gagggacacc cagcttcaga ctggtcccga tcagataata ggtgacaatg
     6001 atgagcgcct gcagcatgcc ggaaagcacc agggcctgtc ccaaggcttg gtggtgccgc
     6061 cgatagcggt gaatggcctc caccattccc tggatcttcg cctggaaccg caccaagccg
     6121 acgagccgca agatgcccgt ggcgcccgtc ttcatccggt cattcaagag caaggtcatg
     6181 gccaggacga aagccccggc cacggccagg accatcccgg tcacctgcgg atcttggagt
     6241 cgtcggtagg cgacggcaag cgccaccagc gcgatgacga ccatggcggc aaagccggag
     6301 agtcgatcca ccaggatcga ggcaatggag gcgtcgtgac cgcgggtgag gcggtagatc
     6361 gtgtagccgc gcacgatatc gcccccgatc aaggtcggga gaaatagatt gaagaaggcg
     6421 gcctcgaagt acaccaggat gaggcgccag aggggcaggt ggatcccctc ggcggcgagg
     6481 aggcgttgcc atcgcagcgt gctgatgacg taccccagcg tgtaaaggcc cacgcatccc
     6541 acgaagacgg agactggcaa ggcttccacg ctgcgcagaa aggcggagcg atccacccgc
     6601 cagaagagaa gccccagcag gcagccgctg accaagagct ttcccaaaat gggcaaccag
     6661 cccgggcgcg gcggctgggg cttcatgggt tttcccggcc ggggtggccc aggatgcgtt
     6721 tgaccatgta gatcggcttc cgttgcgact cgtgatagac gcggacgagc atctcgccca
     6781 ggagccccat gacggtgagt tgcacaccga ccaagatgag caggatcccc aggaagagca
     6841 gcggccggcc gccgatctgg gcgccgaagg cgagcttgag gaccgtgagg tagaggccga
     6901 tgagaatgcc cgcgcccgag gcgagcaggc ccaggagacc aaaaacttga atgggtttgg
     6961 tggcaaagct caggagaaac ttcaccgtga tgagatccaa gatcacccgg agggtccggg
     7021 cgatcccgta cttggagcgg ccgaagcgcc tcggccgatg gtgggtctcg acctcggtca
     7081 gactgatgcc catccagctt gcgatggccg ggatgaagcg gtgcatctcg ccgtagaggt
     7141 ggaggtgttc caccacctcc cgccg
//
LOCUS       DBNGZC010000785         4099 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2022946, whole genome shotgun sequence.
ACCESSION   DBNGZC010000785 DBNGZC010000000
VERSION     DBNGZC010000785.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2022946"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..983)
                     /locus_tag="AC16G0_13450"
     CDS             complement(45..983)
                     /locus_tag="AC16G0_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5100657.1"
                     /translation="MDVARGIQALQTNIEQVLVGKPEVVKFAIVALLARGHLLIEDVP
                     GVGKTTLAQALARSLGLLFQRIQFTSDLLPSDILGVSVFDPRKSEFEFKPGPIFANIV
                     LADEINRTTPKTQSSLLEAMNEAQVSLDHHTYRLPQPFMVLATQNPLEYQGTHVLPES
                     QMDRFLLRIRIGYPDETHERLILRGAGAAALDRLQVVMDAEALLDLQARAGAVKVEES
                     LLQYVMVMVQATRSHPALALGVSPRGSLALVHAAQALALCEGRHFCVPDDIKQLAVAA
                     LAHRVILRTRWDPSAEGRDDAESVIREILHDTPVPR"
     gene            complement(1224..2150)
                     /gene="galU"
                     /locus_tag="AC16G0_13455"
     CDS             complement(1224..2150)
                     /gene="galU"
                     /locus_tag="AC16G0_13455"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-alpha-D-glucose metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HIR5100658.1"
                     /translation="MALPENRPASAVQHRTVRKAVIPAAGMGTRFLPATKSMPKEMLP
                     IVDKPVLQFVIEEAVASGIDDILIVTGRGKRAIENHFDFNPELEAFLATAGKPHLIEQ
                     VRDIGDQAQIHYIRQKEQLGLGDAIRLGRDHVGDQAFAVLLGDTIIDPPEGQKPGLRQ
                     LLDVFEEKQASVVAVHRVPRDWVTRYGIVGGDPERSNHDLVRLRQLVEKPGVDEAPSD
                     LAIAGRYVFTSEIFGCIDATGRGVGGEIQLTDAMNLLAQRQPMYALAWQAKRYDIGNR
                     VEYAKCFIDFALRRPETAKAVRDHLLHILEEA"
     gene            complement(2197..3192)
                     /locus_tag="AC16G0_13460"
     CDS             complement(2197..3192)
                     /locus_tag="AC16G0_13460"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase family protein"
                     /protein_id="HIR5100659.1"
                     /translation="MIIQTKAYARAGLIGNPSDGYFGKTISFIIKNFSARVMLYESPR
                     LTILPQKCDRLEFVNFDELLDDVQVHGYYGGVRLIKAAVKRFGDYCGQIGRRIDQNFT
                     LEYDTDIPIRVGLAGSSAIVTATLRALMEFFGVQIPTPVLPGIILSGELDELKIGAGL
                     QDRVIQVYEGLVFMDFDKERMGRDGFGLYEPMDPTLLPPLFVAYHDSLAEGTEVTHND
                     LRSRFNRGEPQVLDAIKRWAELAQQAHDQLLAGRGREIGPLMDANFDLRASLLQISPG
                     NRRLVETGRRLGAAVKFAGSGGAVVGCYDGDPERLERLATAYAGMGAKLIVPQIA"
     gene            3576..4058
                     /locus_tag="AC16G0_13465"
     CDS             3576..4058
                     /locus_tag="AC16G0_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014652742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR5100660.1"
                     /translation="MGKIETRLKELGIALPRPPKAVANYIPGVKVGEILYVAGTVGTV
                     VGPNDEDVLPIKGKLGQELSIEQGYQSARLTAINHLAMIKAVIGDLDDVVRIVKVIGY
                     VNAAPGFQKAPWVLNGASDLLVEVFGPERGKHARAALYQNELTGDAPIEIEMTVQVKL
                     "
BASE COUNT          748 a         1308 c         1334 g          709 t
ORIGIN      
        1 cgaaggacca cgttcctacc gcctctcgcc tcccggccct cggctcaccg cgggaccggg
       61 gtgtcgtgca ggatttcgcg gatcacagac tcggcgtcgt cgcggccttc tgcgctgggg
      121 tcccagcggg tgcgaaggat cacgcggtgg gccaaggcgg cgacggcgag ctgcttgatg
      181 tcgtcgggga cgcagaagtg ccggccctcg cagagggcca gggcctgcgc cgcatgcacc
      241 agggccaagg acccccgggg gctcaccccg agggccagcg cgggatgact ccgcgtggcc
      301 tggaccatga ccatcacgta ctgcagcagg ctctcctcca ccttcacggc cccggctcgc
      361 gcctggaggt cgagcagcgc ctccgcgtcc atgaccacct gcaggcgatc cagggcggct
      421 gcgcccgcgc cgcgcaggat cagccgctcg tgggtttcat ccggatagcc gatccggatc
      481 cgcaggagga agcggtccat ctgggattcg ggaaggacgt gggttccctg gtactccagg
      541 ggattctgcg tggccaatac catgaacggc tgggggaggc gataggtgtg gtgatccagg
      601 gagacctgag cctcgttcat cgcctccagg agactcgact gggtcttggg cgtggtgcgg
      661 ttgatctcgt cggcaagcac gatgttggcg aatatggggc ccggcttgaa ttcgaactcc
      721 gattttctcg ggtcgaaaac cgacacgccc aggatgtcgg agggaaggag gtcgcttgtg
      781 aactggatgc gctggaagag gaggcccagg gaccgcgcca aggcctgggc caaggtggtc
      841 ttccccacgc ccgggacgtc ctcgatcagg aggtgcccgc gggccagaag ggccacaatg
      901 gcgaacttga cgacctcggg cttgcccacc aggacctgct cgatattggt ctgaagggcc
      961 tgaatgccac gggcaacatc catgtgaacc acgcgctccg atcacggacc cgatgccgaa
     1021 tccgggccgg caggcgaaga gccgccacgg ccggtgattc gagagatcag cctcactcgt
     1081 agaattgatg ctactgttca aaattgggct tggcaagcag ttttggagca gagcccaccc
     1141 acgagcgtga ggggcatggc tgctcgaatt ccagggtcgt tcgagccctc atgctcgggg
     1201 gtgaaccggt cggctgggag gtcttaggct tcttccagga tgtgaaggag atggtcgcgc
     1261 accgccttgg cagtttctgg acggcgcagg gcgaagtcaa tgaagcactt ggcgtactcg
     1321 acccggttgc cgatgtcgta gcgcttggcc tgccaggcca gggcgtacat gggttggcgt
     1381 tgcgccaaga ggttcatggc gtcggtgagc tgaatctccc cgcccacgcc tcggccggtc
     1441 gcgtcgatgc acccgaagat ctcggaggtg aacacgtatc ggccagcgat cgccaagtcg
     1501 ctcggggcct catccacgcc cggcttttcc accagttgac gcaggcggac gaggtcgtgg
     1561 ttgctccgct cggggtcccc acccacgata ccgtaacgcg tcacccaatc cctcggcacc
     1621 cggtgcaccg ccaccacgct ggcctgcttc tcttcgaaca cgtcgagcaa ctggcgcaga
     1681 cccggcttct gcccctccgg cgggtcaatg atcgtatccc ccaagagcac ggcaaacgct
     1741 tgatccccca cgtggtcccg ccccaggcgg atcgcatccc ccaggccgag ctgctccttc
     1801 tggcggatgt agtggatctg cgcctgatcg ccgatgtccc gcacctgctc gatgaggtgg
     1861 ggcttgccgg ccgtggccag gaaggcctcc agctccgggt tgaagtcgaa gtgattctcg
     1921 atggcgcgct tgccgcggcc cgtgacgatg aggatgtcat cgatcccgct ggccaccgcc
     1981 tcctcgatga cgaactgcaa gaccggcttg tccacgatgg gcagcatctc cttgggcatg
     2041 gacttggtcg ccggcagaaa ccgagtgccc atgccggcgg cggggatgac ggccttgcgg
     2101 acggttcgat gttggacggc ggacgctgga cgattctccg gcagtgccat gttcagagtt
     2161 cccctcgatc ccccagaaat ggtaaccggt cacgcgtcag gcgatttgcg gcacgatcag
     2221 ctttgcaccc atcccggcat aggcagtcgc aagccgctcc aggcgttcgg gatccccgtc
     2281 ataacatccg accacagcgc cccccgagcc cgcaaacttg accgccgccc cgaggcgccg
     2341 gcccgtctcg accagccggc ggttgcccgg gctgatctgc aagaggctgg cccggaggtc
     2401 gaagttggcg tccatcagcg ggccgatctc acggccgcgt cccgccagga gctggtcgtg
     2461 cgcctgttgc gccaactccg cccagcgctt gatggcatcc agcacctgcg gctccccccg
     2521 attgaagcgg ctccgcagat cgttgtgggt cacctccgtc ccctcggcga ggctatcgtg
     2581 atacgccacg aaaagcggtg gcaagagcgt cgggtccatc ggctcgtaca gcccgaagcc
     2641 gtcccgcccc atccgttcct tgtcgaaatc catgaagacc aagccctcat agacctggat
     2701 cacccggtcc tgcagcccgg ccccgatctt caattcgtcc agctcgccgc tgaggatgat
     2761 cccgggcaga accggcgtgg ggatctggac cccgaagaac tccatcaggg ctcgcagtgt
     2821 ggcggtgaca atggcgctgg agccggccag ccccacccgg atcgggatat ccgtgtcgta
     2881 ctccagcgtg aaattctggt cgatccggcg gccgatctgc ccacaataat cgccgaagcg
     2941 cttgacggcc gccttgatca ggcggacccc cccgtagtac ccgtgcacct gcacgtcgtc
     3001 cagcaactcg tcgaagttca cgaactccag gcggtcgcac ttctggggca ggatcgtcag
     3061 ccggggcgat tcatacagca tgaccctggc gctgaagttc ttgatgatga acgaaatggt
     3121 cttgccgaag tacccatccg acggattgcc gatgagcccg gcacgagcgt atgctttggt
     3181 ttggatgatc atatgggcct cgcgccactc cccaggtgtg aatctgcagg tttcgctcgg
     3241 aggatgtccc gagccgtaca ccaggaccgc tacggcggaa agcgctctcg gaatgagcag
     3301 gctgctgaaa actcagccgc gcccaggctg ctcaaaatgg accggatgcg aggcagcgtc
     3361 acccatcctt cggatgggta gccggcagat gggcctcttc ggcagcctgg tagacgcaga
     3421 atgccctgca tgcgagacga gtgtcaagag aaaatggcct tctggaggcc tcatcaggtc
     3481 aagcggacct tgacgccgca tcgggttccg ggtatggtgc cgcccacgtc ctagccggta
     3541 gaaagacgag gacctacgtg agaggaggag cacccatggg gaagatcgag acgcgcctga
     3601 aggaattggg aatcgcgctc ccccggccgc ccaaggcggt ggccaattac atcccggggg
     3661 taaaggtcgg ggagatcttg tacgtcgcgg gaacggtcgg aaccgtggtg ggtcccaacg
     3721 acgaggatgt cttgccgatc aaggggaagc tgggccagga gctgtctatc gagcaggggt
     3781 accagtcggc gcgactcacc gccatcaatc acctggccat gatcaaggcc gtgatcggtg
     3841 atctggacga cgtggtccgc attgtcaagg tcatcggcta cgtgaacgcc gcaccgggat
     3901 tccagaaggc gccctgggtg ctgaacggcg cctcggatct cctcgtcgag gtcttcgggc
     3961 cggagcgggg caagcatgcc cgggccgcac tctaccagaa cgagctaacc ggcgacgcac
     4021 ccatcgagat cgagatgacg gtccaggtga aactgtagcc caaagggagt gccagatggc
     4081 caaatcggtg gaggaaatc
//
LOCUS       DBNGZC010000786         1284 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1043451, whole genome shotgun sequence.
ACCESSION   DBNGZC010000786 DBNGZC010000000
VERSION     DBNGZC010000786.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1043451"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..374
                     /locus_tag="AC16G0_13470"
                     /pseudo
     CDS             99..374
                     /locus_tag="AC16G0_13470"
                     /inference="COORDINATES: protein motif:HMM:NF024932.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            454..1188
                     /locus_tag="AC16G0_13475"
     CDS             454..1188
                     /locus_tag="AC16G0_13475"
                     /inference="COORDINATES: protein motif:HMM:NF013753.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR5100661.1"
                     /translation="MASGTATASSSATWSGGAPSGSAAKDRSEASLDEFFTWLGPEKC
                     RKIRVAVMDMWKPFRNSTLRAGHAPQARIIYDKFHVMSHLSKAMDEVRRREYARLSGK
                     DRRFIKGQRYNLLSRRENLTTEGRQALRLLFRANKRLHTAYLLKESFGQLWDYNRPGW
                     ARRFFAHWKDALKWQRLEPFRKFAAMVESHWDGIEAYCHEENKVPLGFVEGLNNKIRS
                     LQGRAYGYRDEEYFRLKILTCMLPKL"
BASE COUNT          235 a          411 c          423 g          215 t
ORIGIN      
        1 cccggcttcc tcccgatggc cgaggttcga ggcgtcttcg gtgacccctg ggccgtggtc
       61 atcaccctcc gccggcgccg aaaaaaacgc gctgtggggt gtgcggacga gggtgtcgca
      121 ccttctacga ccaacggccg cggcgcgtcc gcgacctctc gtgtggcgac cggcgcgtct
      181 acctcgacct ctcggtacga cgggtcgact gccgcgggtg cggtggcgtg aagcgcgaac
      241 ggctggactg gttggcggac aacccgctct ataccaagcg gttcgcgttc tacgtcggga
      301 ggcggtgccg ggagagcacg atccaggcca tcgccgagga gttgcacctg gagtggcaca
      361 ccgtcaagga gctggacaag cagtacatgg gggagcaact ccgccgggcg ggctgcccga
      421 caccgcgggt gatcggcatc gatgagattg ctgttggcaa gcggcacagc taccgcatcg
      481 tcgtcagcga cctggagcgg gggcgcccca tctggttcgg cggcaaagga ccgctccgag
      541 gccagcctgg acgagttctt cacctggctc ggccccgaga aatgccggaa gatccgcgtg
      601 gccgtcatgg acatgtggaa gcccttccgc aactccaccc tcagggcggg ccacgccccg
      661 caggcccgga tcatctacga caagttccac gtcatgagcc acctgagcaa ggcgatggac
      721 gaggtgcggc ggcgggagta tgcccgcctc tcggggaagg accgccggtt catcaagggg
      781 caaaggtaca acctcctctc ccgccgggag aacctgacca ccgagggcag gcaggcgctg
      841 aggctgctgt tccgggccaa caagcggctg catacggcct acctgctgaa ggagtcgttc
      901 ggccagctct gggactacaa ccgccccggc tgggcgcggc ggtttttcgc gcactggaaa
      961 gacgccttga agtggcaacg tctggagccc ttccgcaagt tcgccgcgat ggtcgagagc
     1021 cactgggacg gtatcgaggc gtattgccac gaggagaaca aggtccctct gggctttgtc
     1081 gagggcctca acaacaagat ccggtccctt cagggacgcg cttacggcta ccgtgacgag
     1141 gagtacttcc ggctgaagat ccttacctgc atgctgccga agctctgaaa actacccaca
     1201 cgattccgcg aagagccatt tttctgaatc cgcctcagac cctttcactt gctgacaccg
     1261 ttgcggtgat gaccgcggcg atcg
//
LOCUS       DBNGZC010000787         3178 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1830783, whole genome shotgun sequence.
ACCESSION   DBNGZC010000787 DBNGZC010000000
VERSION     DBNGZC010000787.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1830783"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..907)
                     /locus_tag="AC16G0_13480"
     CDS             complement(89..907)
                     /locus_tag="AC16G0_13480"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIR5100662.1"
                     /translation="MEYTAVARTDVGRKRSGNEDSFCLAQELGLFVVADGMGGHAAGE
                     VASRLAVETILEWMRKYARGESRALVGPPVTSGSEQANWLLSSIRLANRMIFDAARGG
                     AEYAGMGTTLVAALATGPRVALAHVGDSRIYRVRDEQIEQLSRYHSLIQQQLEHGMIS
                     AEEAHDSQYRHLITRALGLKESVEVDLDEQDVLAGDRLLLCSDGLSDLLEDEEMLAAI
                     LEHSDDLEKTCQVLVDRANYKGGDDNITVLLIQAQEGGRAGRGILSWLRRTLTG"
     gene            complement(932..1324)
                     /locus_tag="AC16G0_13485"
     CDS             complement(932..1324)
                     /locus_tag="AC16G0_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HIR5100663.1"
                     /translation="MVRGRHTRRIPDWMIGLAIACVGLGLVWVRPAFLESTENQSFDL
                     CLKWSGSRAPAQNIVIVALDEDSISKLGNCSCPLSRIAALGSVQGDRELLQVLQGVRA
                     FLDNVGRLGGGMQRAVQSRSAAALFFKA"
     gene            complement(1371..1973)
                     /locus_tag="AC16G0_13490"
     CDS             complement(1371..1973)
                     /locus_tag="AC16G0_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014406973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIR5100664.1"
                     /translation="MAVRGMVRHSGIAAPTAWVEGRTGSAVIGVLAFVIMTALGAHVR
                     IPLPWTPVPVTLQTFFVSLAGATLGPALGPASQAIYLLVGVGGLPVFAGGGSGMSYVL
                     SGPTTGYLIGFVASSALVGWLIRRREEPGAAWILLSMTAGSLTIYACGVLWLAWSLGL
                     SFPVAMAKGFLPFLLGDVVKTGAAVGLFRGYRRRVRDVFP"
     gene            complement(2005..2640)
                     /locus_tag="AC16G0_13495"
     CDS             complement(2005..2640)
                     /locus_tag="AC16G0_13495"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR5100665.1"
                     /translation="MQAKEGQGEPPPIGGILFDMDGVVVRQRLDFLAIKREIFGTTEG
                     YILERMAELPPDARVRAESILERHETAAAMAAEPMDGILPFLRWMEARGLRRGIVTRN
                     SRKSVELVLRRLGLVFDAVVTREDAPPKPAPEPVWLACRRMKLSPAELVFVGDFEFDM
                     LAGRRAKVRTVLLRSGVTMTSEHADISVDSLQELRTWLDATGLGVLPKAGD"
     gene            complement(2645..>3178)
                     /locus_tag="AC16G0_13500"
     CDS             complement(2645..>3178)
                     /locus_tag="AC16G0_13500"
                     /inference="COORDINATES: protein motif:HMM:NF013933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HIR5100666.1"
                     /translation="LGALVGPLVVLGHGILEVLLVAGLVLGLGRLLVLGPVIGTIALV
                     GGGVLVWMAWGMFKSIKDLTLRTLSAPGGRRRHPVLAGILTTLSNPYWIVWWATIGLG
                     YIALSMRMGFVGLASFYLGHILSDLAWYGSVSLSLAMGHRLLTDRVYRGLVAVCASFL
                     LAFGLYFGYAGVRYLWV"
BASE COUNT          584 a         1141 c          975 g          478 t
ORIGIN      
        1 tcgaaatcca gttattcgcg cgcaggttcg cctcgttcac ctgttgtcta ttttgccttc
       61 ggctccttgc ttcggctgcc tccgcccgtc agccggtgag ggtccttctg agccaggaca
      121 ggatcccccg tccggcccga cctccctcct gcgcctggat caagaggacc gtgatattgt
      181 catcgccccc cttgtagtta gcccgatcta ccagaacctg gcaggtcttc tcaaggtcgt
      241 ctgagtgctc caggatggcg gccaacatct cctcatcttc caggaggtcc gacagcccgt
      301 ccgaacacag aagcaggcga tcacccgcca ggacgtcctg ctcgtccagg tccacctcga
      361 ccgactcttt cagtccaagg gcgcgcgtga tgaggtggcg atactgggag tcgtgggctt
      421 cctcggccga gatcatgccg tgctccagtt gctgctggat cagcgaatgg tacctggaga
      481 gctgctcgat ctgttcgtcg cggacccgat agatccggct gtcgccgacg tgggccagcg
      541 ccacgcgagg ccccgtggcc aaggccgcga ccaacgtggt ccccatgccg gcgtattccg
      601 cgcccccccg ggccgcatcg aagatcatcc ggttggccag gcgtatgctg ctgagtagcc
      661 agttcgcctg ctcggaaccg ctggtgactg gcggccctac cagggccctc gactctcccc
      721 gcgcgtactt tcgcatccac tccaggatcg tctccacggc aagccggctc gccacctcgc
      781 cggcggcatg gccgcccatc ccgtcggcca ccacgaacag gcccagctcc tgggccaggc
      841 agaagctgtc ctcgttgccg gagcgcttgc gcccaacgtc cgtcctggcg accgcggtgt
      901 actccatgcg cgcctcggtc ctccgggccg ttcaggcctt gaaaaatagg gcggcggcgc
      961 ttctcgactg gactgcccgc tgcatcccgc cgcccagtct gccgacgttg tcgaggaacg
     1021 cccgcacacc ctgcaggacc tgcagcaact ccctgtcgcc ctgcacactc cccagggcgg
     1081 cgattcgcga gagtggacag gaacagttcc ccagcttgct tatggaatcc tcgtcgaggg
     1141 cgacgataac gatattctgc gcgggcgcgc gagacccgga ccacttgagg caaaggtcaa
     1201 agctctgatt ctcagtcgat tccaggaagg caggccgcac ccagaccagg cccagcccca
     1261 cacaggcaat ggccaggccg atcatccagt cgggaatccg acgggtgtgc cgcccacgga
     1321 ccatgctgga tccggcggat ggggacgtcc cccgcccctt ccgtctcctc ctacgggaac
     1381 acgtcccgga cgcggcgtcg atagccacgg aagagcccta ccgccgcgcc ggtcttcacg
     1441 acatccccca acaagaacgg caggaaccct ttggccatgg ccacggggaa cgagagcccg
     1501 aggctccagg ccagccatag gacgccacag gcgtagatcg tcaggctgcc ggccgtcatg
     1561 ctgaggagga tccaagcggc cccgggctcc tcgcgtcggc gaatgagcca cccgacgagg
     1621 gccgaggacg ccacgaagcc aatgagatat ccggtggtgg ggccggaaag cacgtagctc
     1681 atcccgctgc cgcccccggc aaagaccggt aggccgccga ccccgacgag aaggtagata
     1741 gcctggcttg cgggaccgag cgcaggcccc agcgttgcgc cggccaggga cacgaagaac
     1801 gtctggagcg tgaccggcac cggggtccaa ggaagcggga tccggacgtg agcccccagg
     1861 gccgtcatga tcacgaaggc cagcaccccg atcaccgcgc tgccggtgcg cccttccacc
     1921 cacgccgtcg gtgccgcgat cccggagtgt cggaccatcc cccgcactgc catcgagaca
     1981 cctccacccg gatgagaccc cggatcaatc tcccgccttg ggaaggaccc ccagacccgt
     2041 ggcatccagc cacgtccgga gctcctgcag ggaatcgacg ctgatgtcgg cgtgctccga
     2101 ggtcatggtg acgccgctcc gcagaagcac ggtgcgcacc ttcgcccgcc gtcccgccag
     2161 catgtcgaat tcgaagtcgc cgacaaacac cagctccgcc ggcgagagct tcattcgccg
     2221 gcaggccagc cataccggct ccggcgccgg cttgggcggc gcatcttccc gggtgaccac
     2281 cgcgtcgaag acgagcccga gccgtcggag aaccagctcg acgctcttgc ggctgttccg
     2341 ggtcacgatg ccgcgtcgaa gcccccgagc ctccatccac cggaggaagg ggaggatgcc
     2401 gtccatgggc tcggccgcca tggctgcggc cgtctcgtgc cgctccagga tcgactcggc
     2461 gcggacgcgg gcgtccggcg gaagctcggc cattcgttcc agaatgtagc cctcggtcgt
     2521 gccgaagatt tcccgcttga tcgccaggaa atccagccgc tgtcggacga caaccccatc
     2581 catgtcgaaa aggatgccac cgatcggcgg cggttcgccc tgcccctcct tggcttgcat
     2641 caaatcacac ccagaggtac cggaccccgg catagccaaa atacagcccg aatgccagga
     2701 ggaagctagc acacaccgct accagtccgc gatagacgcg atcagtgagc agccggtggc
     2761 ccatggccag gctgaggctg accgaaccgt accacgccag gtccgagagg atatgtccga
     2821 ggtaaaagga ggccaggccg acgaagccca tccgcatgga gagggcgatg tagcccaagc
     2881 cgatggtggc ccaccagacg atccagtagg ggttggacag ggtcgtcaga atccccgcca
     2941 ggactgggtg ccgccggcgg ccgccggggg cgctcaaggt ccggagggtc aggtccttga
     3001 tgctcttgaa catcccccag gccatccaga ccagcacccc accgcccacg agagcgatgg
     3061 tgccgatcac gggcccgagc accagcagcc tccccaggcc cagaaccaat ccagcgacga
     3121 ggaggacttc caggatcccg tgacccaaga cgaccagggg gccgaccagg gcgcccag
//
LOCUS       DBNGZC010000788         4275 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1535336, whole genome shotgun sequence.
ACCESSION   DBNGZC010000788 DBNGZC010000000
VERSION     DBNGZC010000788.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1535336"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /gene="dnaX"
                     /locus_tag="AC16G0_13505"
     CDS             complement(<1..1269)
                     /gene="dnaX"
                     /locus_tag="AC16G0_13505"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR5100667.1"
                     /translation="MSYQVIARRWRPQAFDQIVGQRHVTETLKNAMATDHLAHALLFA
                     GPRGVGKTTTARILAKALNCAGGPTPTPCEKCVACEEIAAGRSVDCIEVDAASNTQVE
                     KTRDLLETVQYAPTRGRHKIYIIDEAHMLSSSSFNALLKTLEEPPPRVVFILATTEPH
                     RIPATIHSRCQRHDFRLLGAAEIVGRLREIVTAEGVTADEGSLAVLTQAAAGSLRDAQ
                     SLLDQAIASMGSHLEAERVAELLGLVKADALAEAAEAILVRDAARALHLVDSLASHGQ
                     DLRQFALELSAHLRDLMILKVCAEPGSLLEGARVSLEISRRQVERTTIPELELMLKTL
                     QQAEAEMRRATQPRFLLEMALIRLAEIGRLQGLQEILERLVALEARPLGGPPAPEPTT
                     QLGLFGQPPGHRTPLVVAPPPGMGGPPPPPA"
     gene            complement(1358..1457)
                     /gene="ffs"
                     /locus_tag="AC16G0_13510"
     ncRNA           complement(1358..1457)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="AC16G0_13510"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(1606..1695)
                     /locus_tag="AC16G0_13515"
     tRNA            complement(1606..1695)
                     /locus_tag="AC16G0_13515"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1659..1661),aa:Ser,seq:gga)
     gene            complement(1817..1907)
                     /locus_tag="AC16G0_13520"
     tRNA            complement(1817..1907)
                     /locus_tag="AC16G0_13520"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1870..1872),aa:Ser,seq:cga)
     gene            complement(2031..2693)
                     /locus_tag="AC16G0_13525"
     CDS             complement(2031..2693)
                     /locus_tag="AC16G0_13525"
                     /inference="COORDINATES: protein motif:HMM:NF021444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
                     /protein_id="HIR5100668.1"
                     /translation="MSDTDERPNTRKLILFGVALAIPLAAVALYFAIFDQPLPTPRAD
                     QAPAAAAASSQSDGEKPALPPNHPPIATQPGNAPSTAGQPGATTGMPPGHPQIGGAGR
                     TVRVPDTVKGKWQAVKLQVEQKTGGAAPQVFTVKLGGQLDIPGSKLRVKVGDFLPALQ
                     VSGSEVTSAGNDPANPAVLVTVSEDGKEIFKGWLFGKFPDMQPFEHPKYKITLLAGVP
                     KG"
     gene            complement(2807..2898)
                     /locus_tag="AC16G0_13530"
     tRNA            complement(2807..2898)
                     /locus_tag="AC16G0_13530"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2862..2864),aa:Ser,seq:gct)
     gene            complement(2981..3066)
                     /locus_tag="AC16G0_13535"
     tRNA            complement(2981..3066)
                     /locus_tag="AC16G0_13535"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3030..3032),aa:Ser,seq:tga)
     gene            complement(3169..>4275)
                     /gene="serS"
                     /locus_tag="AC16G0_13540"
     CDS             complement(3169..>4275)
                     /gene="serS"
                     /locus_tag="AC16G0_13540"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR5100669.1"
                     /translation="LKKAGQDATALIAEMRGVGEQIKKLDEDIRTCDEQIDCFLLSLP
                     NLPHASVPVGQDSSKNPEIRRWGEPRRFDFSPRPHWETGEALGLLDFERASRMAQARF
                     AVLTGLGARLERALINFMLDLHTKEHGYTEVFPPLLVNAAAMTGTGQLPKFAEESFKT
                     KDESLYLIPTAEVPVTNLHREEILPPGTLPLAYVAYTPCFRREAGSYGKDTRGLIRQH
                     QFNKVELVRFAEAGKSYEALEQMTAHAEAVLQRLGLPYRVVVLCTGDMGFAAAKTYDI
                     EVWLPGQPGGGGYREISSISNCEAFQARRANIRYRSAPKAPVEYVHTLNGSGVAVGRT
                     WLAILENYQEADGSVRIPEALRPYMNGLERIIKT"
BASE COUNT          694 a         1421 c         1383 g          777 t
ORIGIN      
        1 ggctggcgga gggggtgggc cccccatccc cggcgggggc gccaccacaa gcggcgtccg
       61 gtgaccaggc ggttggccga agagtccaag ctgcgtcgtc ggctccgggg ccggcgggcc
      121 gcccagagga cgggcctcca gcgccaccaa gcgctccagg atctcctgga gtccttgcag
      181 gcgcccgatc tcggccagac ggatcagggc catttccagc aggaagcgag gctgagtcgc
      241 gcgccgcatt tctgcctctg cctgctggag ggtcttgagc atcagctcca gctcggggat
      301 ggtcgtgcgc tcaacctgcc ttcggctgat ctccagggaa acgcgcgctc cttccaggag
      361 cgatcccggc tcggcgcaca ccttcaggat catcagatcc cggaggtgcg ccgacaactc
      421 cagggcaaat tgtcgcaggt cctgaccgtg agaggccaga ctgtccacca ggtgtagcgc
      481 ccgagccgcg tcccgcacga ggatcgcctc ggctgcctcg gccagcgcgt cggccttgac
      541 caggcccagg agttcggcca cgcgctcagc ctccaggtgg ctgcccatgc tggcgatggc
      601 ctggtccagg aggctctggg catcgcgcag gctgcccgcg gcggcctggg tcagaacggc
      661 cagcgatcct tcgtcggcgg tcaccccttc ggccgtcacg atctcacgca ggcgtccgac
      721 gatctcggcg gcgccgagta gccggaagtc gtggcgctgg cagcgtgagt ggatggtggc
      781 gggaatccga tggggttcgg tggtcgccag gatgaagacc acccgtgggg gcggctcctc
      841 cagcgtcttc aacaacgcgt tgaacgaact gctggagagc atgtgagctt cgtcgatgat
      901 atagatcttg tgccggcctc gtgtgggggc gtactgaact gtctccaaga ggtcgcgggt
      961 tttttcgacc tgggtgttcg atgccgcgtc cacctcgatg cagtccaccg aacggccggc
     1021 cgcgatctcc tcgcaggcca cgcacttctc gcagggtgtc ggcgttgggc ccccggcgca
     1081 attgagggcc ttggccagta tacgggccgt ggtcgtcttt ccgacccccc gcggcccggc
     1141 gaacagcaat gcgtgggcca gatggtccgt agccatggcg ttcttcagcg tctcggtgac
     1201 atggcgctgc ccgacgatct gatcgaaagc ctgtggccgc cagcgcctgg cgatgacttg
     1261 atatgacatc ggcaccctgt tggacggaag actgtcagtt cctggccgcg ggccggcggc
     1321 ggcaggccag agaagagcgc cgggcggctc atgcagctcc gaccgggtgg actcacggca
     1381 ctggccgctc tctgcttacc gttgcttcct tccggatctg gcggggttca cagggctacc
     1441 ttgcgcgaga cccgatcatc aacacggcac gaaccgtccg gcgctcacct gaacctcggg
     1501 cgacgcagcc ttcgtctccc gaggaggatg ttcacttgat cgagttgcct gtcctccgat
     1561 ccgcggtccg accgccggtc cccgggtgca gtcggacgcg gcgactggcg gagaggccgg
     1621 gattcgaacc cggggtggag tttcccccac acacgctctc caggcgtgct ccttcaacca
     1681 ctcggacacc tctcctcacg ccagcaacca cttgctcacc gcgggtggcc gggccgccgc
     1741 ggtccgtgac cggaagaagc ctcagaatcg tgtctgaccg agcccaccgt gaacggccga
     1801 tccgctcgac cagcgatggc ggagagggtg ggattcgaac ccacgtggga gttgcccccc
     1861 aacccgcttt cgaggcgggc ccgttacgac cacttcggta cctctcctac ccgaaaccca
     1921 cgggctcatc cgaattccag aaccgacgct ccgcgaccgg ccatccgctc cgactcgcga
     1981 aagcggccgg ttgtccagcg tcggtttcca atacccaatt ccgggagaat ctaacccttt
     2041 ggtacacctg cgagaagcgt gatcttgtac ttcggatgct cgaagggctg catgtccggg
     2101 aacttgccaa acagccagcc cttgaatatc tcctttccat cctctgacac cgtcaccagg
     2161 accgccggat ttgccgggtc gttccctgcc gaggtgacct cgctgccgct cacctggaga
     2221 gccggaagga agtcgccaac cttgacgcgc aacttgctcc ccgggatgtc aagttggcct
     2281 cccagcttca ccgtgaagac ttgcggcgcg gccccgcctg tcttctgctc gacctggagc
     2341 tttacggcct gccacttccc cttgactgta tcggggaccc ggactgtgcg gccggcgccc
     2401 ccaatctggg gatggccggg cggcatcccg gtggtagcac cgggttgacc ggcagtggac
     2461 ggcgcgttac cgggctgggt ggcaatcggg ggatggttcg gaggcagcgc cggtttttcc
     2521 ccatcgctct ggctgcttgc ggccgcggcg gctggcgcct ggtcggcccg aggcgtgggt
     2581 aacggctgat cgaagatggc aaagtacagg gcaaccgccg ccaggggaat ggccagtgcc
     2641 acgccgaaca ggatgagctt tcgtgtgttc ggccgctcgt ctgtatcgct catgctcttg
     2701 cctcgacacc tcggattgcg ggcgccagat cgagtgcatc cggatccttc tgggtcgtcg
     2761 ggcgacgggg tctgcgctgg cacaccagct gacgcttcaa tggaattggc ggagaggccg
     2821 ggattcgaac ccgggggcca gttttacccg accaaccgct tagcaggcgg ttgccttcgg
     2881 ccgctcggcc acctctccgt ttcgtggaat acactctgcg gtcttgtacc gaccggacga
     2941 cccgcctgac gcatgcgccg cgcgtcggat ccccgtgcgg tggcggaggg ggtaggattc
     3001 gaacccacgg accttgcggt catcccgttt caagcgggac gccttagacc actcggccac
     3061 ccctccggga atccggagcc ggagatcccg cccatccaga cgaccggccg ccgtaacctc
     3121 gtcatgatag aaaacatcgg cgcgaaaggg aagcggaaac gtgcgggtct atgtcttgat
     3181 gatccgctcg agcccgttca tgtacgggcg caaggcctca ggaatgcgaa cgctgccatc
     3241 tgcctcctga taattctcca ggatggcgag ccaggtccgg cccacggcca ctcccgagcc
     3301 attcagcgtg tgcacgtact ccacgggagc tttgggggcg gagcgatagc ggatgttcgc
     3361 ccgtcgcgcc tgaaaggcct cacagttact gatggaggag atctcccggt agccgccccc
     3421 gccgggctgg ccgggaagcc agacctcgat atcgtaggtc ttggcggcgg cgaatcccat
     3481 gtcccccgtg cacaggacga ccacgcggta ggggaggccc aagcgctgga gcaccgcttc
     3541 ggcatgtgcg gtcatctgct ccagcgcctc gtaggacttg ccggcctcgg cgaacctcac
     3601 caactccacc ttgttgaact ggtgctgtcg gatcagccct cgggtatcct ttccatagga
     3661 ccccgcttcg cggcggaagc atggtgtata ggccacatat gcgagtggca acgtcccagg
     3721 cggcagaatc tcctcccggt gcaggttggt cacggggacc tcggcggtcg ggatcaggta
     3781 gagcgactcg tccttggtct tgaacgattc ctcggcgaac ttggggagtt gtcctgttcc
     3841 ggtcatggca gccgcattca ccaggagtgg cgggaagacc tcagtatacc cgtgctcctt
     3901 ggtgtgaagg tccagcatga aattgatcag cgcccgctcc agcctggccc ccaaaccggt
     3961 cagcacggcg aagcgcgctt gggccatgcg cgaggcccgc tcgaagtcca ggagacccag
     4021 cgcctctccg gtctcccagt gcggcctggg agagaagtcg aaccgacggg gctcccccca
     4081 gcgacggatc tccgggttct tgctactatc ctgccccacg ggcaccgagg catgggggag
     4141 gtttggaagg ctcagcagga agcaatcgat ctgctcgtcg caggtccgga tgtcctcgtc
     4201 aagcttcttg atctgctcac ccacccctcg catctcggct atgagggcgg tggcgtcctg
     4261 tcccgccttc ttcag
//
LOCUS       DBNGZC010000789         2665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1631853, whole genome shotgun sequence.
ACCESSION   DBNGZC010000789 DBNGZC010000000
VERSION     DBNGZC010000789.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1631853"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="AC16G0_13545"
     CDS             <1..894
                     /locus_tag="AC16G0_13545"
                     /inference="COORDINATES: protein motif:HMM:NF013974.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate oxidoreductase subunit alpha"
                     /protein_id="HIR5100670.1"
                     /translation="VPLQMAIADRALDPPGDFGSEHTDAMSTRDQGWILGWAENPQEA
                     FDNCLINYRVGEDHRVLLPQMVCQDGYFVSHIPGKVVLPEQSQVDEFLPPYKLPHPLD
                     PHHPVSHGPQIRPDQGTIMDLQRAQAMLDTPAVIKEAIEDFNRIFGRTYSPFVEEFMT
                     DGAEMVFFLQGAHTMTARHAVQHLRAKGVKIGLVKLRFVRPWPTDDVAGILSKFKAVG
                     VIETSTSYGGAMRGGNLLHEVRASLYDAPQRPLTTSFMAGLGGEMVPLKEFYWMAEVM
                     SNALKAGKMRKNVYWVGFEEE"
     gene            981..1961
                     /locus_tag="AC16G0_13550"
     CDS             981..1961
                     /locus_tag="AC16G0_13550"
                     /EC_number="1.2.7.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004099106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate oxidoreductase subunit beta"
                     /protein_id="HIR5100671.1"
                     /translation="MAVAVKTQNLPMIKKIRNIPKDEMYVPGHRTCAGCGPALQYRLV
                     AKAAGPNTIFLGPTGCMYVANASYLCTPYRVPWMHTQITNGGAVASGIEAAYQVLIRK
                     GKWEGELPNIIVMAGDGGSVDIGLQAMSAMMYRGHDALFVCYDNESYANTGIQTSPTS
                     PYGANTTFTPPGALIPEGKKLFPKDPPQLVIGGHPAVKYVATASVAYPVDLMNKVRKA
                     LNVVGPTFLHIHAPCPKGWKFDESKTVEIAKLAVATGMWNLYEWENGEYKYQHKPKEY
                     KPVREYMKLQKRFAHLTEAHIAKMQAFVDAKLKAPGMPVEIPVPGPRQQA"
BASE COUNT          541 a          807 c          859 g          458 t
ORIGIN      
        1 gtgccgctgc agatggcgat tgccgaccgc gccctggatc ccccaggcga cttcgggtcg
       61 gagcacaccg acgccatgtc cacccgagat cagggttgga tcctcggctg ggcggagaac
      121 ccgcaggaag ctttcgacaa ctgcctgatc aactaccggg taggcgagga ccatcgggtg
      181 ctcctgcccc agatggtgtg tcaggacggc tatttcgtct cccacattcc ggggaaggtg
      241 gtcctcccgg agcagagcca ggtggacgag ttcctgccgc cctacaaact gccccatccg
      301 ctggatcccc atcacccagt ctctcacggg ccgcagatcc gcccggacca ggggacgatc
      361 atggacctgc agagggcgca ggccatgctg gacacccccg cggtgatcaa agaggccatc
      421 gaggacttca accggatctt tggccgcacg tattctcctt tcgtggaaga gttcatgacc
      481 gacggcgccg agatggtgtt cttcctccag ggcgcccaca ccatgacagc ccgccacgcg
      541 gtccagcacc tgcgggccaa gggtgtgaag atcggcctgg tgaaactccg gtttgtccgg
      601 ccatggccga ccgatgacgt ggcgggtatc ctgtcgaagt tcaaggccgt gggcgtgatc
      661 gagaccagca cgtcctacgg cggcgccatg cggggcggca acctgctgca cgaggtccgc
      721 gcgtccctct acgacgcccc ccagcggccc ctgacgacct cgttcatggc cggcctgggt
      781 ggcgagatgg tgccgttgaa ggagttctac tggatggccg aggtcatgtc caatgccctg
      841 aaggccggca agatgcggaa gaacgtgtac tgggtcggct tcgaggagga gtagagcgca
      901 agtcgtgtcg agtgaaacgt gagcgtgaat catggcggca tgattcatga taggcgacga
      961 gcgaacgaaa ggagccagca atggcggttg ccgtgaagac acaaaacctt ccgatgatca
     1021 agaagatccg gaacattccc aaggacgaga tgtacgtccc gggccaccgg acctgcgccg
     1081 ggtgcggccc ggccctgcag tatcgactcg tggccaaggc ggcaggcccg aacaccatat
     1141 tcctcgggcc caccggctgc atgtacgtgg ccaacgccag ctacctctgc accccctatc
     1201 gtgtgccctg gatgcacacc cagatcacca acggcggggc ggtggcttcg ggtatcgagg
     1261 cggcctatca agtgctgatc cgcaagggga agtgggaggg cgagttgccc aacatcatcg
     1321 tcatggccgg tgacggcggc tcggtggaca tcggcctcca ggcgatgtcg gccatgatgt
     1381 accgcgggca cgacgccctg ttcgtctgct acgacaacga gtcgtatgcg aacaccggga
     1441 tccagacatc cccgaccagc ccgtacgggg ccaacaccac cttcacgcct ccgggagccc
     1501 tgatccctga gggcaagaag ctcttcccga aggatccccc acagctcgtc atcggcggcc
     1561 atccggcggt gaagtacgtg gccacggcct ccgtggccta ccccgtggac ctcatgaaca
     1621 aggtccgcaa ggcgttgaac gtggtggggc ccaccttcct ccacatccac gccccctgcc
     1681 cgaagggctg gaagttcgac gagtccaaga cggtcgagat cgccaagctg gccgtggcga
     1741 ccggcatgtg gaacctgtat gagtgggaga acggcgagta caagtaccag cacaagccga
     1801 aggagtacaa gcctgtccgc gagtacatga agctgcagaa gcggttcgcg catctcaccg
     1861 aggcccacat cgccaagatg caggccttcg tggatgccaa actgaaggca ccgggcatgc
     1921 cggtggaaat cccggttccg ggaccgcggc agcaggctta acgaacctcg ctggaagcca
     1981 ccgagggagt tttcggatag gtctgatggc gtcggactct cgaggcagag cagggcggag
     2041 ggaaggctcc aggcagtcgg cccaacaggc tgcgtggggc tgtccccctc cgccctcctc
     2101 gcttcggagg attgttcact tccatgtgaa taatccaggt cagcaatcgg tctacgattc
     2161 agaaccgggg agaggttctg cgtgacaggg taagggagag caagtcaatt ctcggtggca
     2221 gcccattcgt gcaaccccca cggcatgagc attatactag gtgcggcaga ctggttctct
     2281 tggaggcatt cgggatcctc gaaagactgc ctgcttcacc gcggggactg caatgtgtcg
     2341 caattcccct gaacaatctt gattgtgcaa gaggtatcag gggaacgcag ctgctgtcaa
     2401 gcgtccggcc tcacctgggg cggccggcgg tgatgtgggt gggttcccat aacggatccg
     2461 atcacccatc atgtgacgat ttcgtgagag gctgaagttc gagcactcaa gaagtgcgca
     2521 gcctcccgga tgtggaggaa ggaggtgggg aatcagagaa gggccggccc ctgagaaacg
     2581 cagccaagtc gattcaggtg tggtgaccgg aggtgtaact acctaagcgg tcaacctgcg
     2641 gtgttccggt tagcggaggt ccgtg
//
LOCUS       DBNGZC010000790         1383 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_950302, whole genome shotgun sequence.
ACCESSION   DBNGZC010000790 DBNGZC010000000
VERSION     DBNGZC010000790.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1383
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_950302"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="AC16G0_13555"
     CDS             <1..516
                     /locus_tag="AC16G0_13555"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5100672.1"
                     /translation="ISAMFQREAWVVGYLEKEAPKLNYGVFPMPKGPGGWGTVGNTMG
                     LAVSKSSKVKKEAFAFAKWMMEGPQALFQYDYTGWQPFPTAYDYSSLYKKKPALKDFI
                     VYSNTPGHTVYDYENMPAISEVHNRMAERIMAAFKRADLKDNPQGIAKVVSETAEETN
                     RVLADHGMLAK"
     gene            544..>1383
                     /locus_tag="AC16G0_13560"
     CDS             544..>1383
                     /locus_tag="AC16G0_13560"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5100673.1"
                     /translation="MASQGSSVSTAPAHATEAGALQGAPARRRGGSLRALRKSQLAFL
                     LWIFAPVIALFVIIRVIPIFVILLFSFTNYSITRAFWRWTGLVNFERLIGDNNFLVAL
                     WNSTVYVLVAVPAEVLLGLFFAVLLSRRVRFESFYETLYFLPYIIPMVPAAIIWKWIY
                     APGSFGLANHYLELLGFGRVGWLTNPSIALLAVIGMHVWKNLGFFVIIFLVGLKNVPN
                     DVKEAASIDGASQWAQVLRVELPLLKPIILFGAVMATIWAWSAFTEVYIMTQGTDIST
                     GTEI"
BASE COUNT          244 a          442 c          430 g          267 t
ORIGIN      
        1 atctcggcga tgtttcagcg tgaggcctgg gtggtgggat acctggagaa agaggcgccg
       61 aagctgaatt acggggtctt cccgatgccg aagggcccgg gcggctgggg cacggtcggc
      121 aacaccatgg ggctggcggt gtcgaagtcc agcaaggtga agaaggaggc cttcgccttc
      181 gcgaagtgga tgatggaagg cccgcaggcc ctcttccagt atgactacac cggctggcaa
      241 cccttcccga ccgcctacga ctacagcagc ctctacaaga agaagcccgc cctcaaggat
      301 ttcatcgtgt actccaacac gccgggccac acggtgtacg actacgagaa catgcccgcc
      361 atctccgagg tccacaaccg gatggcggag cggatcatgg ccgccttcaa gcgcgcggac
      421 ctgaaagaca acccccaggg gatcgccaag gtcgtgagcg aaacggctga ggagacgaac
      481 cgggtcctgg cggaccacgg gatgctagcg aagtaacccc gaccggggtt gctcgggggt
      541 ctcatggcct cacagggcag ttcagtgtcc acggcaccgg cacacgcgac ggaagcgggg
      601 gccttacagg gggcccccgc ccgtcggcgg ggcggctcgc tccgcgccct tcgcaagagc
      661 cagttggcct tcctgctgtg gatcttcgcg cccgtcatcg cgctcttcgt catcatccgc
      721 gtgatcccga tcttcgtcat cctgctgttt tcgttcacga actattccat cactagggcc
      781 ttctggcggt ggacgggcct ggtgaacttc gagcgcctga tcggcgacaa caacttcctc
      841 gtggcgctct ggaactcgac cgtgtacgtc ctcgtggccg tcccggcaga agtcctgctg
      901 gggctcttct tcgcggtcct gctcagtcgc cgcgtccgtt tcgaatcctt ctacgagacg
      961 ctctacttcc ttccgtacat cattcccatg gtgcccgccg ccatcatctg gaagtggatc
     1021 tacgctcccg gatcgtttgg cctggccaat cactacctgg agctgctcgg attcggccgg
     1081 gtgggctggc tgaccaatcc cagcatcgcc ctgctggcag tcatcgggat gcacgtctgg
     1141 aagaacctgg ggttcttcgt catcatcttc ctggtggggc tcaagaacgt ccccaacgat
     1201 gtgaaggaag cggcgtccat cgacggcgcg tcgcagtggg cacaggtcct gcgggtggag
     1261 ctgcccctcc tgaagccgat catcctcttt ggcgccgtca tggcgacgat ctgggcctgg
     1321 tcggccttca cggaggtcta catcatgacc cagggcacgg atatctccac ggggacggag
     1381 atc
//
LOCUS       DBNGZC010000791         2367 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1438780, whole genome shotgun sequence.
ACCESSION   DBNGZC010000791 DBNGZC010000000
VERSION     DBNGZC010000791.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1438780"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..680
                     /locus_tag="AC16G0_13565"
     CDS             198..680
                     /locus_tag="AC16G0_13565"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR5100674.1"
                     /translation="MRMLNRLIWKVLEVVVLTAFVAMLSLVIAQVFFRYVLQVSVPWT
                     EEAARWFYAWQIFLGSAIATKENLHLRADFLLKRFPPRARACADFVISLAGLTFLAGV
                     IWGSLLMIRAVYPVEAGSFPVSTSYLYLSIPVSLLVMFCLTAKDLTRDCRTLFSRMRG
                     "
     gene            696..1988
                     /locus_tag="AC16G0_13570"
     CDS             696..1988
                     /locus_tag="AC16G0_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR5100675.1"
                     /translation="MLIFFLMMGALLVLMLLGFPVALSMGVTSVLTLGVLRGFTHIPW
                     DMLAQRIMYGVTNFTLLAIPFFILAGRLCNEAKITDRIFEFAKCLVGHFKGGLGHVNV
                     LGSMIFAGMSGSAVADAGGLGQMEIKAMVDEGYSLEFSTAVTAASATIGPIIPPSIPM
                     VIYGAMAGESVAKLLIAGFFPGIIMGAAMMALLVVMAPWYGFQAKPRARLREMWAALK
                     SGVLPLLAPVILIGGIVSGVFTPTEAAAIAVAYATVMGIFYRTLTLRSFLQVLRDSMM
                     DTSIILFIVGASSIYSWVIARYQITDVLVNWMGGIVSTPLMFLFVVNIFLLLVGCFID
                     PTPALFILVPVFMPMVKQYGIDPIHFGVIMVFNLMIGLVTPPVGTVLYTLNRVTGLSL
                     ERNSEAMLPWYIPLLVTLVLIVLFPSISLVLPTALLGK"
     gene            2128..>2367
                     /locus_tag="AC16G0_13575"
     CDS             2128..>2367
                     /locus_tag="AC16G0_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100676.1"
                     /translation="MTEEQDLDGVSRVAAFKGVAAFKAHLDTLPIAMACDDELLPPDR
                     SPLGEPLSVYGRTVSNRIVTHPMEGWDARLDGKPSE"
BASE COUNT          361 a          784 c          736 g          486 t
ORIGIN      
        1 ccagcagggg gcgcccgagg tgaagcacct ccttaaagag atcctcggtt tctagccgtc
       61 gtccatcgac agggagcccg gcgtccgccc gttcttcgct cgggcggcgc gccgggctct
      121 ccggccccat cgcggccacg acggcccccg ccgtccgtcg ccacggaatc cctcagcgac
      181 gcgaaacggg tttgcccatg cgcatgctca accgtctcat ctggaaggtt ctggaggtcg
      241 tggtcctgac cgccttcgtg gccatgcttt ccctcgtgat cgcccaggtc tttttccgct
      301 acgtcctgca ggtgtccgtc ccctggaccg aggaagccgc gcgctggttc tatgcatggc
      361 agatcttcct cggcagcgcg attgccacga aggagaacct gcacctgcgg gccgacttcc
      421 tcttgaagcg gttcccgccc agggcgcgcg cctgcgcgga cttcgtcatc agtctggccg
      481 gcctcacctt tctggcgggg gtcatctggg gaagccttct catgattcgg gcagtctatc
      541 cggtggaggc cggcagcttc cccgtcagca cgagctacct ctacctgtcc atccccgtga
      601 gcctcctcgt catgttctgc ctgacggcga aagatctcac ccgggattgc cggacgctgt
      661 tcagccggat gcggggctga ggggagggcg gggcgatgct catcttcttc ctgatgatgg
      721 gggccctgct ggtcctgatg ctcctcggct tccccgtggc gctgagcatg ggagtaacaa
      781 gcgtcctcac gctgggcgtg ttgcgggggt tcacgcacat cccctgggac atgctggccc
      841 agcgcatcat gtatggcgtg accaacttca ccctgctggc cattcccttt ttcatccttg
      901 ccggccgcct ctgcaatgag gccaagatca cggaccggat cttcgagttc gccaagtgcc
      961 tggtggggca cttcaaaggg ggcctgggcc acgtgaatgt cctgggcagc atgatcttcg
     1021 ccggcatgtc cgggtcggca gtggcggacg ccggcggcct gggccagatg gaaatcaagg
     1081 cgatggtgga cgagggctac agcctggagt tcagcaccgc ggtgacggcg gcctcggcga
     1141 ccatcggccc catcataccg cccagcatcc ccatggtgat ctacggggcc atggcgggcg
     1201 aatcggtggc caagctcctc atcgccggct tcttcccagg catcatcatg ggggcggcca
     1261 tgatggccct cctcgtggtg atggcgccct ggtacggatt ccaggcgaag ccgcgcgccc
     1321 gtctccggga gatgtgggcc gccctcaaga gcggagtcct tcccctcctg gccccggtga
     1381 tcctgatcgg aggcatcgtc tcgggggtgt tcacgcccac agaggccgcg gccatcgccg
     1441 tggcctacgc gaccgtcatg ggcattttct accgcaccct gaccctgagg agcttcctgc
     1501 aggtcctccg ggactccatg atggatacgt cgatcatcct gttcatcgtc ggcgccagct
     1561 ccatctatag ctgggtcatc gcccgctatc agattaccga tgtgctggtg aactggatgg
     1621 gcgggatcgt cagcaccccg ctgatgttcc ttttcgtcgt caacatcttt ctcctgctcg
     1681 tcggctgctt catcgatccg acgcccgcgc tgttcatcct ggtgcccgtg ttcatgccca
     1741 tggtcaagca atacggcatc gaccccattc acttcggggt catcatggtt ttcaacctca
     1801 tgatcggcct ggtcacgccg ccggtcggca cggtcctgta caccctcaac cgggtgacgg
     1861 ggctctccct ggagcggaac tccgaggcca tgctgccctg gtacatcccg ctcctggtga
     1921 cgctcgtcct catcgtcctg ttccccagca tctcgctggt cctgccgacc gccctgctcg
     1981 gaaagtagcg caggcgggga agcgtccccc ggagagtcca ttcgggagaa aaggcttgcc
     2041 tcgaatgggc tacaccagta tagacatacg gcttctttgc gtcgggcatg cggcaccacc
     2101 gcgttttggg ggaggattgg tttctccatg acggaagaac aggatctaga cggcgtttcc
     2161 cgtgtggcag cgttcaaggg tgtggctgcg ttcaaagcgc atctggacac cctgcccatc
     2221 gccatggcct gtgacgacga gcttctccca ccggatcgga gtccgctggg ggaacccctg
     2281 agcgtgtacg ggcggacggt gagcaaccgg attgtcaccc accccatgga gggctgggac
     2341 gccaggctgg acgggaagcc gtcggag
//
LOCUS       DBNGZC010000792         2537 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1730100, whole genome shotgun sequence.
ACCESSION   DBNGZC010000792 DBNGZC010000000
VERSION     DBNGZC010000792.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1730100"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="AC16G0_13580"
     CDS             complement(<1..713)
                     /locus_tag="AC16G0_13580"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5100677.1"
                     /translation="MLAVERGRGPRSLFRIYLLLLLATLFWGGSQVAGKLAIREIPPM
                     TAGVFRYGIACLVLLALFWRRLPSWADLSRQDRWYLLGLGILGTFLNHICSYLGLFLA
                     PASHAAILGPTSSPICTLALAAGFAGERLTRGQISGMVLCMLGVILGARPGGPSTAMT
                     RTVLLGDLLLVLSGLAWGAYSFLSKIAMRRMSAMTALTYGMAMGTVLLVPVGLVERPW
                     AALAGASALSWASLLYIIVA"
     gene            complement(722..>2537)
                     /locus_tag="AC16G0_13585"
     CDS             complement(722..>2537)
                     /locus_tag="AC16G0_13585"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5100678.1"
                     /translation="YPETLHVNFSGEIVAMTLVGGMRNFFGPIVGAVFFIFARDILSS
                     YTENWMVFFGIVFMAFILFSPNGITGATLALWGRLRSRRGAGRREETQVATGGPPAAM
                     AASETAVPRVIGEPILTTQGLVKRFGALAAVDGVDLTVRRGELHSIIGPNGAGKTTFF
                     NALFGILDSDAGSAQFKGERITGLRPDQIVSRGISRSFQIISVFQELTVLENVRIAVQ
                     ARSPHRFDLLSRADNLTGLVEEAGRIIGLVGLGGKEEMAASSLSHGDQRLLEIGIALA
                     TGPEVLLLDEPLAGLSPRERIRVADLIRQLAERVTVVLIEHDIDQVLALSDRITVLHQ
                     GKVIAEGTPAEIQKDPQVQVAYLGGLKVSEVEPRAASSAAPLLSVESINTLYGKSHIL
                     HDVSLEVREGEVVCFLGRNGAGKTTTLGSIMGTAPPRSGAIRFGGQSIAGAPPEEIGG
                     LGIGLVPKGRRIFPNLTVMENLLIARRNGKGRWDAGSVFRLFPKLEQLKARRGEHLSG
                     GELQMLAIARALMGNPRLLLLDEPFEGLAPAVVEGVWRALAEIRGTTTLLLVEQNADL
                     ALALGDRAYVINNGRIEHSGPSHTLMHDHDLRKRLLGV"
BASE COUNT          432 a          883 c          845 g          377 t
ORIGIN      
        1 gccacgatga tgtagaggag cgaggcccag gagagggcgg aggcgccggc gagcgcagcc
       61 cagggtcgct ccaccagtcc caccgggacc aggaggacgg tccccatggc catcccgtag
      121 gtcaatgcgg tcatggcgct cattcgccgc atggcgatct tggacagaaa cgagtaggcg
      181 ccccacgcca gcccgctcag gaccaagagg agatccccca ggagaactgt ccgcgtcatg
      241 gcggtggacg gaccgcccgg cctggcaccc aggatgactc cgagcatgca gagcaccatc
      301 ccgctgatct gcccgcgggt caggcgctct ccagcgaaac ccgctgccag cgccagggtg
      361 cagatcgggc tgctggtggg acccaggatc gccgcgtggg aggcgggcgc caggaagagg
      421 cccaggtagg aacagatgtg attaaggaac gtccccagga ttcctagacc caggaggtac
      481 cagcgatcct gccggctcaa gtccgcccat gacggcaggc gtcgccagaa cagggccagc
      541 aggacgaggc aggctatgcc gtaccggaag acgccagcgg tcatgggcgg gatctcgcga
      601 atggcgagct ttcccgccac ctgcgatccg ccccagaaaa gcgtggccag cagcaggagc
      661 agatagatcc ggaagaggga cctcggcccg cgccccctct cgacggcgag caccgccggc
      721 ctcacacacc cagcagccgc ttccgcaggt cgtggtcgtg catgagggtg tggctggggc
      781 cgctgtgctc gattcggccg ttgttgatga cgtaggcccg gtcccccagg gccagcgcca
      841 ggtcggcatt ctgctcgacc aggagcagcg tcgtggttcc ccggatctcg gccagggcgc
      901 gccacacccc ctccaccacc gccggggcga gcccctcgaa cggctcgtcc agcaggagga
      961 gccgtgggtt tcccatgagc gccctggcaa tggccagcat ctgcagctcc ccgccgctca
     1021 ggtgctcgcc gcgccgggcc ttcaactgct ccagcttggg gaacagtcga aacaccgacc
     1081 ccgcgtccca ccggcctttg ccgttccgcc gagcgatgag caggttctcc atgacggtga
     1141 gattggggaa gatgcgccgg cccttgggga ccaggccgat ccccagacca ccgatctcct
     1201 cgggcggggc gcccgcgatg gattgcccgc caaatcggat ggcgccgctc cggggcgggg
     1261 ccgttcccat gatgctgccg agcgtggtcg ttttcccggc cccgttgcgg ccgaggaagc
     1321 agaccacctc gccttcccgg acctccagcg acacgtcgtg caggatgtgg ctctttccgt
     1381 acagcgtgtt gatgctctcc acgctgagca ggggggcggc cgacgaggcg gcccggggct
     1441 cgacctcgct caccttgagg ccgcccaggt aggccacctg cacctgcggg tccttctgga
     1501 tctccgccgg cgtcccctcg gcgatcacct tgccctgatg caggacggtg atccgatccg
     1561 agagggccag gacctggtcg atgtcgtgct cgatcagcac gacggtgacc cgctcggcga
     1621 gctgccggat gagatcggcg acccggatgc gctcgcgggg cgagagcccc gccagcggtt
     1681 cgtccagcaa gagcacctct gggcctgtgg ccagggcaat gccgatctcc agcaagcgct
     1741 gatccccgtg ggacaggctg ctcgcggcca tctcttcctt gccgccgagc cccaccaggc
     1801 cgatgatcct cccggcctcc tccaccagac ccgtgaggtt gtcggcgcgg gacaggaggt
     1861 cgaagcggtg cgggctccgg gcttgcaccg cgatgcgcac gttctccagg accgtcagct
     1921 cctggaacac gctgatgatc tggaacgacc gggagatccc gcgggagacg atctggtcgg
     1981 gtcggagacc cgtgatgcgc tcccccttga actgggccga cccggcgtcg ctgtccagga
     2041 tcccaaacag ggcgttgaag aaggtggtct tgccggcccc attgggtccg atgatcgagt
     2101 gcagttcccc ccgccgcacc gtcaggtcca ccccgtccac cgcggccagg gcgccgaatc
     2161 gtttcaccaa accctgggtg gtcagtatgg gttcgccgat gacgcgcggc acggccgtct
     2221 cggaggccgc catggctgct gggggacctc ccgtggccac ctgcgtttcc tcccgacgcc
     2281 ccgccccccg gcgggagcgg agccgccccc agagagccag ggtggcgccg gtgatcccat
     2341 tcggcgagaa caggatgaag gccatgaaca cgatcccgaa gaacaccatc caattttccg
     2401 tgtagctgct caggatgtcc cgggcgaaga tgaagaacac agcgccgacg atcggtccga
     2461 agaagttccg catcccgcca accagggtca tggccacgat ctctcccgag aagttcacgt
     2521 gcagcgtctc ggggtag
//
LOCUS       DBNGZC010000793         4155 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_459370, whole genome shotgun sequence.
ACCESSION   DBNGZC010000793 DBNGZC010000000
VERSION     DBNGZC010000793.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_459370"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="AC16G0_13590"
     CDS             complement(<1..433)
                     /locus_tag="AC16G0_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR5100679.1"
                     /translation="MTVFEASVLGLVQGLTEFLPISSIAHLRVIPALLGWLDPGAAFS
                     AVIQLGTLVAVLIYFAGDLWDLVRAAVRGVRAGRPWGSHEARLAWFILLGTLPIGLCG
                     VAFKRSIVGEWRSLYVVATSLILLGLLLWLAERTAAFHRSIG"
     gene            complement(465..857)
                     /locus_tag="AC16G0_13595"
     CDS             complement(465..857)
                     /locus_tag="AC16G0_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014453759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR5100680.1"
                     /translation="MAEFTVRTRATQEMVDITGRVAELVKATGVEDGICVIYVPHATA
                     AVVINENADPNVCADILDALAGLIPEGRWRHDRIDNNAAAHIKATILGPSEAVPVQGG
                     RLRLGTWQSLMLVELDGPRDRSVIVEVR"
     gene            1046..1324
                     /locus_tag="AC16G0_13600"
     CDS             1046..1324
                     /locus_tag="AC16G0_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100681.1"
                     /translation="MPEEATRRLLKLFGMAVTDLEEQTQRTLEQIHAMGPRPDHAPIA
                     LEIVEQWLTLSREVTERWLDMTRLIVENQAKMQTELLRMLADGRRAGK"
     gene            1365..1580
                     /locus_tag="AC16G0_13605"
     CDS             1365..1580
                     /locus_tag="AC16G0_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100682.1"
                     /translation="MDPKIREEVETAVRALDEALGGLITFTMTLRPSLRNEILQICGH
                     HLERARQAKERLDALLQTGDRPQDGPT"
     gene            1818..2126
                     /locus_tag="AC16G0_13610"
     CDS             1818..2126
                     /locus_tag="AC16G0_13610"
                     /inference="COORDINATES: protein motif:HMM:NF025178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR5100683.1"
                     /translation="MGKLVKVAETRDLAPGQGKLVEVDGQPIALFNVNGVFYAVGAVC
                     PHEGGPLQDGELEDDTVICPWHSFDYNLKTGKCSVDPTLSVATFVVKTEGDDVFIEVA
                     "
     gene            2153..2461
                     /locus_tag="AC16G0_13615"
     CDS             2153..2461
                     /locus_tag="AC16G0_13615"
                     /inference="COORDINATES: protein motif:HMM:NF025178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR5100684.1"
                     /translation="MATFVKVGRVSDFSEGQVKVVQANGQRIALFHVGGRFYALEAAC
                     PHEGEPLADGTIDGLRVLCPWHGYDFHLKTGECELDPDLHALTYPVKVHRKEILIEMA
                     "
     gene            complement(2583..3509)
                     /locus_tag="AC16G0_13620"
     CDS             complement(2583..3509)
                     /locus_tag="AC16G0_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5100685.1"
                     /translation="MNTDLGELQVMDAHAHFFSHPFFMQFVDALRQELPGDDPYGGLA
                     RRLGWELPTPDHAALGRRWVQEMDEHGLERMVLIASMPGDEESILAAARAFPDRILGY
                     FMLDPTRPDAAERTRRSLAAGLRGVCLFPAMHRFHVWEERCYPVFEAAATAGAVVFTH
                     FGILKMGIRDKLGLPSKFDMRYSNPIDLHRVAKDFPATVFVMPHFGCGFLREALIVGD
                     QCANVCVDTSSSNAWVNTMPTPLDLTQVFGAALRVFGPERILFGTDSTYLPRGWRHDI
                     FDAQVQVLKTLQVPVQHARLIFRGNLARVLKI"
     gene            complement(3617..>4155)
                     /locus_tag="AC16G0_13625"
     CDS             complement(3617..>4155)
                     /locus_tag="AC16G0_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100686.1"
                     /translation="AALAVSGLVVAVVGLRHQAAEPAFGRWAKAYGGRWFAGATILQF
                     GSGIWFLSVQPPRVRDALLGGSPEDSSLLLAAVGLALVALALLAFPSRISSLRFSLGS
                     GAIGLTVILMVLLRQRVRTLWLLPHFRYEALPIASQWGAILLFLILLLIGISLVGWML
                     WRFFRQPARGSVEGGGQA"
BASE COUNT          835 a         1373 c         1283 g          664 t
ORIGIN      
        1 tgccgatgct gcggtggaag gctgcagttc tctccgcaag ccagaggagc aggccaagaa
       61 gaatcaagct cgttgccacc acgtacaggg aacgccactc gcccacgatg gatcgcttga
      121 acgccacccc gcacagcccg atgggcagcg tgcccagcag gatgaaccag gccaggcgcg
      181 cctcatggct cccccagggg cgtccagcgc gaaccccgcg gaccgccgcc cggaccaagt
      241 cccatagatc gccggcaaag tagatcagca ctgccaccag cgtgccaagc tggatgaccg
      301 ccgagaaggc cgcgccaggg tccaaccatc caaggagtgc cgggatgact cgcaggtggg
      361 cgatgctgct gatgggtaga aactccgtga gcccttggac cagacccaga acactggcct
      421 cgaagacggt catgcctcct ccctcaggcc tcctgaccgg cccctcatcg gacctcgacg
      481 atcaccgacc gatcccgcgg gccgtccagt tccacaagca tcaggctctg ccacgtcccg
      541 agcctgaggc gtcccccctg gacaggaacc gcctcgctgg gacccaggat cgtcgccttg
      601 atgtgcgccg cggcattatt gtcaatccgg tcatgtcgcc accggccctc cgggatcagt
      661 cccgccaggg catccaggat gtccgcacac acgttcgggt cggcgttctc attgatgacc
      721 accgctgccg tggcatgggg gacatagatc acacagatgc catcttcgac tcccgtggcc
      781 ttgaccagct ccgccacccg accggtgatg tccaccatct cctgggttgc ccgcgtgcgg
      841 acggtgaatt cggccatgcg aagacctcag gaggcaacgg cgatgacgat cattcacccc
      901 gaacccattc tggactcggc gcctgtactg gcatatgcta cggggcgatt caggcgcgcg
      961 taactatact cacgcccgcg gaccttgtca cgggaaaatc gggcgggagc attggccctt
     1021 caggggagaa agaagaggaa gtcctatgcc ggaggaggca actcgcaggc tgctgaagct
     1081 tttcggcatg gccgtgaccg atcttgaaga gcagacccag cgcaccctgg agcaaataca
     1141 cgccatgggt ccgcggcccg accacgcgcc gatcgccttg gagatcgtcg agcagtggct
     1201 cacgctgagc cgtgaggtga cggaacgctg gctggacatg accaggctga tcgtggagaa
     1261 ccaggccaag atgcagaccg aactcctgcg catgctcgcc gatgggcgca gggccgggaa
     1321 ataagcagga ccgacccctt tccccatccc gtggcggagg ctggatggat ccgaagatcc
     1381 gcgaggaagt ggagaccgcc gtccgggcat tggacgaggc actgggtggc ctcatcacct
     1441 tcacgatgac cttgcggccc agtctgcgaa acgagatcct gcagatctgc ggtcaccacc
     1501 tggagcgagc ccgccaggcc aaggaacggc tcgatgccct gttgcaaaca ggtgatcgac
     1561 cccaggacgg tccgacctga cggaacacat ccaggcgctt cgaaccacaa ccgctgccct
     1621 gtgcatcgcc caggcttccc ctgtcgcgac tgagactccc acaatgttga acgatccctc
     1681 cagtctgaga cccgatctcc tccgcatcac tgcgttcgcg ccgggcggag tattgccttc
     1741 cgcaggatcc tatgttagcg tgtcgccatt cgagcctcgc acggaagcat tcaccctaac
     1801 cacgaggatg gcgagccatg gggaaactcg tcaaggtggc tgaaactcgc gatctcgctc
     1861 cgggtcaggg taagttggta gaagtggacg gccagcccat cgccctcttc aacgtgaatg
     1921 gcgtcttcta cgccgtgggc gccgtctgtc cacacgaagg ggggccgctg caggacggcg
     1981 agctggagga cgacacggtc atttgtccct ggcacagctt cgactataac ctgaagaccg
     2041 gcaagtgctc ggtggatcca acactcagcg tcgcgacctt cgtcgtcaag accgagggcg
     2101 acgacgtgtt catcgaagtg gcctgaagct gggcttcgga tcggctccgc acatggcgac
     2161 atttgtcaag gtgggtcgag tgagcgattt ctcggagggc caggtcaagg tggtccaggc
     2221 gaacggccag cgaatcgccc ttttccacgt ggggggacga ttctacgccc tggaggcggc
     2281 ctgcccccat gagggggagc cgctggcgga cggaacgatc gatggcctgc gggttctctg
     2341 cccctggcac ggatacgatt ttcacctcaa gaccggggag tgcgagcttg atcccgacct
     2401 ccatgccctg acctacccgg tcaaggtgca ccggaaggag atcctgatcg agatggcctg
     2461 accctctgcg ttcttcttgc gcctgaatga atcggcccga tccggtatca gcggatcggg
     2521 cctttgtctt ttggaaaatc tgtgtaatct gcgaaatctg cggttacatg gatttctggt
     2581 cttcagatct tcagcacgcg cgccaggttc cccctgaaga tcagccgggc gtgctgcacc
     2641 ggaacctgca acgtcttcag gacttgcacc tgcgcgtcga atatgtcgtg acgccacccc
     2701 cgcgggaggt aggttgaatc ggtgccgaac aggatccgct ccggcccgaa gacgcggagc
     2761 gccgccccga agacctgcgt gaggtccagc ggagtgggca tggtattcac ccaggcgttg
     2821 gagctggagg tatccacgca cacgttggcg cattgatctc caacgatcaa cgcctcgcgc
     2881 agaaagccgc agccgaagtg gggcatgacg aataccgtcg cggggaagtc cttcgccacg
     2941 cgatgcaggt cgatcggatt cgagtagcgc atgtcgaact tgctgggcaa ccccaacttg
     3001 tcccggatcc ccatcttcaa gatgccgaag tgcgtgaaga caacggcccc cgccgtggcc
     3061 gccgcctcga acacgggata gcaccgctcc tcccacacgt ggaagcgatg catcgccggg
     3121 aacaggcaga cgccgcggag gccggccgcc agggaccgcc tggtccgttc ggccgcatcc
     3181 ggcctggtcg ggtcgagcat gaagtacccg aggatgcggt cggggaaggc acgcgctgcc
     3241 gccaggatgg actcctcgtc tcccggcatg ctggcaatga gcaccattcg ctcgagaccg
     3301 tgctcgtcca tctcctggac ccagcgcctc cccagggccg cgtggtcggg cgtgggcagc
     3361 tcccatccca gccgacgtgc gagcccgccg tacgggtcgt caccgggcaa ctcctgcctg
     3421 agtgcatcca cgaactgcat gaagaacgga tgggagaaga aatgggcgtg ggcatccatc
     3481 acctgcagct ctccgaggtc ggtattcacg atcccccctc cctgagtgtc gcaaaggacc
     3541 gccttctccc gcaatggtcc ccagctcaga agtcacctgg ccatgccgtc gccgcgcaga
     3601 cagataatgg aatgagtcag gcctgcccac cgccctccac ggaccctcga gccggctgcc
     3661 ggaagaaccg ccacagcatc caacccacga ggctgatccc gatcaacagg aggatgagga
     3721 aaagcaggat ggccccccac tgcgaggcaa tcggcagcgc ttcgtagcgg aagtgcggca
     3781 agagccagag ggtccgcacc cgctgacgca ggaggaccat caatatgacc gtgaggccga
     3841 tggcaccgga gccgagggag aaccgcaggc tcgagatccg gctggggaaa gccaagagcg
     3901 ccaaggccac cagagcaagt ccgactgcgg ccaggaggag ggaggagtcc tctggactcc
     3961 cccccaacag ggcatcccgc acccgcggtg gctggaccga aaggaaccag atccccgagc
     4021 cgaactggag gatcgtggct ccggcaaacc agcgtccccc gtacgccttg gcccatcggc
     4081 cgaaggcggg ctccgccgcc tggtgccgaa ggcccacgac ggcaacaacc agtccgctca
     4141 ccgccaatgc cgcca
//
LOCUS       DBNGZC010000794         1718 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2221040, whole genome shotgun sequence.
ACCESSION   DBNGZC010000794 DBNGZC010000000
VERSION     DBNGZC010000794.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2221040"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="AC16G0_13630"
     CDS             complement(<1..695)
                     /locus_tag="AC16G0_13630"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5100687.1"
                     /translation="MPIADHALDDIEDKVLQGERLSFSDGVRLVRQATPLQLSAWADS
                     VRRRLHPDDRVTYVIGRNINSTTVCWVQCKFCNFYRLPGAEGGYVLPQEGIFAKIREL
                     MALGGTEILIQGGLNPALRIEYFEESFRAIKANFPVHIHGLSPAEIIYLAKISGLDER
                     TCLIRLKAAGLDSIPGAGAEMLVDEVRDQIAPYKDSAAAWLKLMRTAHQLGIPSTVTM
                     MYGSVETVEQRVE"
     gene            complement(715..>1718)
                     /locus_tag="AC16G0_13635"
     CDS             complement(715..>1718)
                     /locus_tag="AC16G0_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CofH family radical SAM protein"
                     /protein_id="HIR5100688.1"
                     /translation="NLARERRSGHRTYYVRNLHINYTNICNKLCKFCSFYTAPNSTDG
                     RGYVLAPEEAAARVAQFCREPIREIHMVAGINPKLPYEYYLELLRAVKAARPEAQIKA
                     FTMIELAQIARAAKRPLEAILPELRAAGLTACPGGGAEVFSERVHEELFRAKLDNQGW
                     FEVARKVHAAGIPSNATLLYGHIETTDEKVRHLLHIRELQDETGGFMCFVPLAFDPTN
                     TELSHLPATTGYANLREIAVARLLLDNFPHVKAFWIMITPAVAQMALWYGADDIDGTV
                     SHYEITHALGTTSHRQVLGSGELLDLITEAGRVPVERDALYNLIQPEEAVSLQPSAG"
BASE COUNT          292 a          534 c          582 g          310 t
ORIGIN      
        1 tgctcgacgc gctgctctac cgtctccacc gagccataca tcatggtgac cgtggagggg
       61 atgccgagct ggtgggccgt ccgcatgagc ttcaaccacg cggccgccga atccttgtag
      121 ggagcgatct ggtcccggac ctcgtccacc agcatctccg cccccgcccc ggggatggaa
      181 tccaggccgg cggctttgag ccggatgaga caggtccgct cgtcgagccc ggagatcttg
      241 gccagataga tgatctcggc cggcgagagg ccatggatgt gaaccggaaa gtttgccttg
      301 atggcccgga aggattcctc gaagtactcg atccggagcg ccgggttcag gcctccctgg
      361 atcaggatct cggtcccgcc cagcgccatc agctcccgaa tcttggcgaa gatcccctcc
      421 tgcggaagga cgtagcctcc ctcagcgccc ggcagccggt agaagttgca gaacttgcac
      481 tgcacccagc agacggtggt ggagttgatg ttccggccga tcacgtaagt gacgcgatca
      541 tcggggtgga gacggcgacg gaccgaatcg gcccaggcag aaagctggag aggggtggcc
      601 tggcggacga ggcggacgcc atccgagaag gacagacgct cgccctgcag aaccttgtct
      661 tcgatatcgt cgagggcgtg gtcggcaatt ggcatggcgg tcggcagtca ggattcagcc
      721 cgctgacggc tgaaggctga cggcctcttc cggctggatg agattgtaca gggcgtcccg
      781 ctcgaccggc acacgtccgg cctcggtgat gagatccagc agctcgcccg accctaggac
      841 ctgccggtgg gaggtcgtcc ccaaggcgtg ggtgatctcg tagtgcgaga ccgtcccgtc
      901 gatgtcgtcc gccccgtacc acagcgccat ctgcgccacc gccggcgtga tcatgatcca
      961 gaaggccttg acgtgaggaa agttgtccag caagagtctg gcgacggcaa tttcgcgcag
     1021 gttggcgtag ccggtagtcg ctggcaggtg ggacaactcc gtgttggtcg ggtcgaaggc
     1081 cagcggcacg aagcacataa agccgccggt ctcgtcctgc agttcccgga tgtgaagcag
     1141 gtgacggacc ttctcgtcgg tggtctcgat gtggccgtac aggagggtgg cattggacgg
     1201 gatacccgcg gcatggacct tcctggcgac ctcgaaccag ccctggttgt ccagcttggc
     1261 ccggaacagc tcctcgtgga cccgctcgct gaatacctcc gccccacctc cggggcaggc
     1321 ggtgagtccc gccgcccgga gctcgggcag gatggcctcc aggggacgct ttgcggcgcg
     1381 cgcgatctgg gccagctcga tcatcgtgaa cgccttgatc tgcgcctcgg ggcgcgccgc
     1441 cttcaccgcg cgcagcagct ccaggtagta ctcataggga agcttggggt tgatcccggc
     1501 caccatgtgg atctcccgga tcggctccct gcagaactgg gccactcggg cggcagcctc
     1561 ctccggcgcc aggacatagc cgcggccgtc cgtgctgttg ggcgcggtgt agaacgagca
     1621 gaacttgcag agcttgttgc agatgttggt ataattgatg tggaggttgc ggacgtagta
     1681 ggttcggtgg ccgctccgcc gttcccgcgc caggtttg
//
LOCUS       DBNGZC010000795         1707 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2511278, whole genome shotgun sequence.
ACCESSION   DBNGZC010000795 DBNGZC010000000
VERSION     DBNGZC010000795.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2511278"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="AC16G0_13640"
     CDS             <1..410
                     /locus_tag="AC16G0_13640"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5100689.1"
                     /translation="KKRGSGQIVNIAALSGRVPRLGQIESNEAKAGLINFTESLAAEV
                     ARHGIRVNAVCPAAILTERWQAKVTRYGEEHALPFERAMEELASKAIPLGRLGMPEEV
                     AGAVVFLASDRAAFITGISIFVDGGMGRSVSVP"
     gene            451..1476
                     /locus_tag="AC16G0_13645"
     CDS             451..1476
                     /locus_tag="AC16G0_13645"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5100690.1"
                     /translation="MRRWSVLALMLAFFWPAGPAGLTFAQDARLEAARKEGKVVWYTS
                     LAVPSTEKVAKLFEAAYPGIKVEAHRTGSERVLQRLMQELQANIKNADVVHTSDAGHF
                     VLLKQKKLLMQYTPAGVEKFPAGFKDRDGYHYGLRSTLSVIAYNSKLVPAAEAPRSWK
                     DLLDPRWRGKLVTGHPGYSGVISTHVLALVNLYGWDFFKQMAQNKLMLVQSANDPAQV
                     VASGERPVAVDGSEYFFYQTKKKGNPIEIVYPKEGVPLVVSPSAITSFAPHPNAAKLF
                     TDFIFGKEVQQLLADSEGLYTGHPEVSYPPDKPKLGELKLLPADPEELEKRNEELKTR
                     FVEFFGA"
BASE COUNT          320 a          529 c          561 g          297 t
ORIGIN      
        1 tgaagaagcg gggaagcggg cagatcgtca acatcgcggc cctctccggg cgggtgccgc
       61 gcctgggtca gatcgagtcg aacgaggcaa aagccggcct gatcaatttc accgagtcgc
      121 tggccgccga ggtggccagg cacgggattc gggtcaatgc cgtctgtccc gctgccatcc
      181 tcacggagcg gtggcaggcg aaggtgacgc ggtatgggga ggaacacgca ctcccttttg
      241 agcgggcgat ggaggagttg gcgagcaagg ccatcccgct gggccgcttg ggcatgcccg
      301 aagaggtcgc tggcgccgtc gtcttcttag cctcggatcg cgcggctttc atcacaggta
      361 tctcgatctt tgtggacggg ggtatggggc gctcggtctc cgtcccgtga caagatcacc
      421 gagtagccaa gggggagagg gggaagggtc atgcgaaggt ggtcggtcct cgcgctaatg
      481 ctggcattct tctggcccgc cggtcccgcg ggcctgacat tcgcgcaaga tgcccgcctg
      541 gaggccgcca ggaaagaggg gaaggtcgtc tggtacacct ccctggcggt ccccagcacg
      601 gagaaggtcg ccaagctgtt cgaggcggcc tatcccggca tcaaggtcga ggctcaccgg
      661 acaggctcgg agcgggtcct gcagcggctg atgcaggagc ttcaggccaa catcaagaat
      721 gccgacgtgg tgcacacctc tgacgcgggc catttcgtcc tcctgaagca gaagaagctg
      781 ctgatgcagt acacgcccgc gggggtggag aagtttccgg ccgggttcaa ggatcgggac
      841 ggctatcact acggtctccg atccaccctg tcggtgatcg cctacaactc caagctcgtc
      901 ccagcggccg aggctccaag gagctggaag gacctgctgg acccaaggtg gcggggaaag
      961 ctggtgacgg gtcaccccgg ctacagcggg gtcatctcca ctcacgtcct ggcgttggtc
     1021 aacctgtacg gctgggactt cttcaagcag atggcccaga acaagctcat gctggtccag
     1081 tcggctaacg acccggcgca agtggtcgcc tccggagagc gccccgttgc ggtggatggc
     1141 tccgaatact tcttctatca aaccaagaag aaggggaacc ccatcgagat cgtttacccg
     1201 aaggaagggg ttcccctcgt cgtgtccccc agcgccatca ccagcttcgc cccgcacccc
     1261 aatgccgcca agctgttcac cgacttcatc ttcggcaagg aggtgcagca gctcctggca
     1321 gacagtgaag gactctacac cggccatccg gaggtgagct acccgccgga taaaccgaag
     1381 ctgggggagc tgaaacttct tccggcagat cccgaggagc tggagaagcg gaacgaggag
     1441 ctcaagactc gcttcgtgga gttcttcggc gcataacgag gaggccgcct gccatcagcg
     1501 gtcagccgtt ggtctcagag ccaagctgac cgctgatggc cgacgacgaa tggccaaccc
     1561 gatggctcag gccgcacccg cttttccgtc acgtctcgag gggatggatc gatccgtccc
     1621 cgtatgggcc ctggcggctc tgatcctggc cctcctgatc ctcctccccc tcggctggct
     1681 ggcctacgtc agcgtgagca gcgaggc
//
LOCUS       DBNGZC010000796         6225 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2709637, whole genome shotgun sequence.
ACCESSION   DBNGZC010000796 DBNGZC010000000
VERSION     DBNGZC010000796.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2709637"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /gene="purQ"
                     /locus_tag="AC16G0_13650"
     CDS             complement(<1..686)
                     /gene="purQ"
                     /locus_tag="AC16G0_13650"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HIR5100691.1"
                     /translation="MKVGVLIFPGTWSHQDFAHVLQDVLKVDWGYVWHKDTSVAGYDC
                     LILPGGFAHGDYLRSGAIARFSPIMEAVVRFARDGGPVMGSCNGFQILCESGLLPGAL
                     LRNTSLQYRCRWVHIRAEQTGTPFTRTLRPGQVLRIPISHGEGRYHADAEALARLKTQ
                     GQIVFRYSAPDGGLTDEANPNGSLEHIAGVCNEGRNVLGLMPHPERAAEALLGSTDGI
                     PLFRGILEALL"
     gene            complement(683..937)
                     /gene="purS"
                     /locus_tag="AC16G0_13655"
     CDS             complement(683..937)
                     /gene="purS"
                     /locus_tag="AC16G0_13655"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HIR5100692.1"
                     /translation="MIEAHVQVTLKKSVLDPQGETVRGALKTLGFQGVEDCRVGKFMV
                     LRLKETDTVQAKTQVEEMCRKLLANPVIEEYVFQLREVAG"
     gene            complement(1072..1800)
                     /gene="purC"
                     /locus_tag="AC16G0_13660"
     CDS             complement(1072..1800)
                     /gene="purC"
                     /locus_tag="AC16G0_13660"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR5100693.1"
                     /translation="MRDGRMATFRRGRKLYEGKWKILYETRDPALLIQQFKDAPTFAA
                     QSVRRDLPKRGSMKNQISAALFGLLQKNGIPTHFVEALGGREMLVRRLEMIRLEVVVR
                     DIAAGSLAKRLGMEVGTPLPVPVLECYYKSDALGDPLLNEDHVRVLGLASLEELRVIR
                     QMAFRANSILLPFFAEREILLADFKLEFGRQKGGLMLGDELTPDGCRFWDRTTRESLG
                     KDRARRSAKGEAAAYQEIFRRVCA"
     gene            complement(1809..3104)
                     /gene="purB"
                     /locus_tag="AC16G0_13665"
     CDS             complement(1809..3104)
                     /gene="purB"
                     /locus_tag="AC16G0_13665"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR5100694.1"
                     /translation="MIPRYSRPSMTAIWEPANRFALWLKIEILACEAWHELGEVPADA
                     LEEIRARAGFDIARIDAIEREVKHDVVAFLTAVAERVGPAARYLHLGLTSSDILDTAL
                     ACQMRQSADLLLTDVDGLAATLTELATKHRKTVMIGRTHGIHAEPTTFGLKLAMWLME
                     VRRARERLLRAREVVSFGKLSGAVGTFANVPPRVEEYVCARLNLSPEPVSTQIIQRDR
                     HAEFLAALAIVGASLDKFATEVRHLQRTEVREVEEPFSEGQKGSSSMPHKRNPVACEQ
                     ISGLSRLLRSNAQAGLENVALWHERDISHSSVERVILPDSTILLDYLLVRFREILEGL
                     RVYPENMRRNLEATHGVIFSQQVLLTLARAGASREAAYRIVQRNAMRAWEEGQPFRQL
                     LQEDPEVRAILGEAEIESCFDLGYHLRHVDDIFRRVGLA"
     gene            complement(3497..4729)
                     /locus_tag="AC16G0_13670"
     CDS             complement(3497..4729)
                     /locus_tag="AC16G0_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR5100695.1"
                     /translation="MIEHFQKEVLDNGIVILTEHMPQVRSVSIGVWVKVGSRFEKAER
                     AGISHFIEHLLFKGTESRSAEEIARAIDSVGGTLDAFTSRENTCFYAKVLGEHLPLAV
                     DLLSDLILHPRLDPEDLEKERRVVLEEIKMVEDDPDDLIHDLFTQQFWRDHPLGRPVL
                     GSRQTLQAITREDVREHLRDFYQPDRVIVAAAGDLDHAKVTALVRSAFGGWQGQAVVA
                     NGKSPVSYLTVHHEDRDSAQLHLVLGAEGLPYNHPSRYAFFLLNAILGSSMSSRLFQE
                     IREKRGLAYSIYSYQASYQDSGLMAVYAGTSAESYRQVVDLIRAEFARLRTESVDLEE
                     FQRAKEQLKGNLLLGLESTSSRMTRLAKAEIYFQRYFDLDEIIRGIDGVAPQDFAELN
                     RSLLEPDRYALTTIGRLN"
     gene            complement(4726..>6225)
                     /gene="pnp"
                     /locus_tag="AC16G0_13675"
     CDS             complement(4726..>6225)
                     /gene="pnp"
                     /locus_tag="AC16G0_13675"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR5100696.1"
                     /translation="LARQMGVNKPVFSPPPVDAGLQARVEDLCRSRIRELAFVPEKEE
                     QKARRKVLLDEALALAATEPEAAQLQVKEIFEELEKQEMRRLILDKGRRADGRTTTQV
                     RPITCEIGVLPRTHGSALFTRGQTQALATTTLGTSEDEQRMDDLEGESFKRFMLHYNF
                     PPFSVGEVKFMRGASRRDIGHGALAERAIQPVLPGKEEFPYTVRIVSDILESNGSSSM
                     ATVCGASLSLMDAGVPVRAAVAGVAMGLVKEGDKAVVLTDIQGLEDHLGDMDFKVAGT
                     RRGVTSIQMDMKIQGITEAIMRQALTQAQAARMEVLDIMDRAIAKPRTALSPYAPRII
                     SFKIPVDKIREVIGPGGKVIRGIVEQTGVKIDIEDDGTVAIASVDEAAAQKAMDIIGK
                     IVEVPEVGKVYLGKVVKITDFGAFVEILPGTDGLLHISQISQERVKRVEDVLREGEEV
                     MVKVIEVDKSGKIRLSRRELLKEAAEKAEGPARTGGEDRQRGGRKPSER"
BASE COUNT         1022 a         2141 c         1979 g         1083 t
ORIGIN      
        1 aggagagcct ccaggatccc acggaagagg ggtatgccgt ccgtggaacc cagaagggcc
       61 tcggcggccc gctccgggtg cggcatgagc cccaggacat tgcggccttc gttacacacc
      121 ccagcgatat gctcgagcga cccgttcggg ttcgcctcat ccgtgagtcc cccgtccggg
      181 gcgctgtagc ggaagacgat ctgtccctgg gtcttcagac gggcgagtgc ttcggcgtcc
      241 gcgtggtacc gtccttcgcc gtgagagatg gggatcctca acacctgccc cggccggagg
      301 gtccgggtga agggcgtgcc cgtctgctcc gcccggatgt gcacccagcg gcaacgatac
      361 tggagggacg tgttgcgcag aagcgccccg ggcaagagcc ctgactcgca cagaatctga
      421 aagccgttac agcttcccat gacgggcccg ccgtccctgg cgaaccggac caccgcctcc
      481 atgatgggcg agaagcgggc gatggcccca gagcggaggt agtcgccgtg ggcgaacccg
      541 ccaggcagga tcagacaatc gtaacccgcc accgaggtgt ccttgtgcca gacgtacccc
      601 cagtccacct tgaggacatc ctggaggacg tgggcgaaat cctggtgact ccaggtgccc
      661 gggaagatga ggaccccgac cttcatcctg ccacctcacg cagctggaag acatactctt
      721 cgatcaccgg gttcgccagg agcttccggc acatctcctc cacctgagtc ttggcctgga
      781 cggtgtcggt ctccttcagg cgcaggacca tgaatttccc gacgcggcag tcctccaccc
      841 cctggaagcc cagggtcttc agggcgcccc ggacggtctc accctggggg tcaaggacgc
      901 tcttcttgag cgtgacctgg acatgggctt caatcatgga tgacctcgcg acacagttgg
      961 aggcagggcc tcgcccaaac ggcggtccgc tttttgggcg attcgatcgt tcggtggtta
     1021 cagaacaccg gcagcgctgt acccccggac cagcatcccc tcttgatcag gtcaggcgca
     1081 cacccgccgg aagatctcct ggtaggccgc cgcctcgcct ttggcgctac gccgggcgcg
     1141 gtccttcccc aaactctccc gggtggtccg atcccagaag cggcagccgt ccggcgtcag
     1201 ctcgtcgccc agcatgaggc cccccttctg ccggccgaac tccagcttga aatcggccag
     1261 caggatctcc cgctcggcga agaacggcag caggatgctg ttcgcccgaa acgccatttg
     1321 ccggattacc cgcagctcct cgagggaggc caggcccagc acccggacgt gatcctcgtt
     1381 cagcagcggg tcacccaggg catcactctt gtagtaacac tccaggacgg ggaccggcag
     1441 gggcgtgccg acctccatcc ccaggcgctt ggccaggctg ccggccgcga tgtcccggac
     1501 gaccacctcc aaccggatca tctccagccg gcgcaccagc atctcccgac cacccagggc
     1561 ttccacgaag tgggtgggga tcccgttctt ctggagcaag ccgaagagcg ccgccgagat
     1621 ttggttcttc atgctcccgc gcttcggcag atcccgccgg acgctctggg cggcgaaggt
     1681 cggggcgtct ttgaactgct ggatgaggag ggctgggtcc cgcgtctcgt acaggatctt
     1741 ccactttccc tcgtacagct tgcggccgcg ccggaaggtc gccatccgcc cgtccctcac
     1801 cacccgcctc aggccagccc gacgcggcgg aagatgtcgt ccacgtggcg cagatgatat
     1861 cccagatcga agcagctttc aatttccgcc tcgcccagga tggcccgcac ctcggggtcc
     1921 tcctggagga gctgcctgaa cggctggcct tcttcccagg ctcgcatggc gttccgctgc
     1981 acgatccggt aggccgcctc ccgggaggcc ccggcgcggg ccagggtcag caggacctgt
     2041 tgggagaaga tcaccccgtg ggtggcttcg aggtttcggc gcatgttttc gggatacacc
     2101 cgcagcccct ccaggatttc gcggaagcgc accaggaggt agtccagcag gatcgtgctg
     2161 tcgggtagga tcacccgctc caccgaggag tggctgatgt cccgctcgtg ccagagggcc
     2221 acgttctcca ggccggcctg ggcgttgctc cgcaacagac gcgacagccc cgagatctgt
     2281 tcgcaggcca cggggttccg cttgtggggc atggacgagg agcccttctg tccctcggaa
     2341 aaaggctctt ccacctcccg gacctcggtg cgctgcaggt gacggacctc ggtcgcgaac
     2401 ttgtccaggg aggcccccac gatggccagg gccgccagga actcggcgtg gcggtcgcgc
     2461 tggatgatct gggtggagac gggttcgggc gagaggttca atctggcaca gacgtactcc
     2521 tccacgcggg gcgggacgtt ggcgaaggtc cccacggcgc cggacagctt gccgaagctc
     2581 accacctccc gcgcccgtag caggcgctcc cgcgcccgtc gcacctccat cagccacatg
     2641 gccagcttga gaccgaaggt cgtgggttcg gcgtgaatgc cgtgggtgcg gccgatcatg
     2701 accgtcttcc ggtgcttcgt cgccaactcg gtcagggtcg cggccagacc gtccacgtcg
     2761 gtcagcagga gatccgccga ctgtcgcatc tgacaggcca gcgccgtgtc caggatgtcg
     2821 gaggacgtga gcccgaggtg gaggtaccgg gccgccggcc ccacccgttc cgccaccgcc
     2881 gtgagaaagg ccacgacgtc atgcttcacc tctcgctcga tggcgtcgat gcgcgcgatg
     2941 tcgaagcccg ccctggcccg gatctcctcc agggcgtccg cggggacctc ccccagctcg
     3001 tgccaggcct cgcaggccag gatctcgatc ttgagccaca aggcgaaccg gttggcgggc
     3061 tcccagatgg cggtcatcga gggtcgggag taccggggga tcatgcgacc tcctcttgag
     3121 gggtacgagg ttggccggaa cgcgccgctg cagttcagaa ggttggtttc aggcttcgag
     3181 gtccgagatg ctgcaggtga aacccaggaa ccttgaacct cgcaccggca accgtcggtc
     3241 cagacagcaa accccgaatc tcgaaccgac gcgcaacgca aacaccggca gcctaccaca
     3301 gactgacggc ggagacaatg gcatccgcgc gcgatccagc caacacccca gatgaagctg
     3361 gcgggacccg accctcgatc cagaccaacg ctgctcccct cacacggaca cagggccagc
     3421 ctgtagattc tctgcctttg tcgattggta cgggcgatcg ggcggaagga agcgcgtgca
     3481 gcaggcgggc ccgaaatcag ttgaggcggc cgatggtggt cagggcgtag cggtccggct
     3541 ccaagagcga ccggttcagc tcggcgaagt cctgtggcgc gaccccgtcg ataccccgga
     3601 tgatctcgtc caggtcgaag tagcgctgga agtagatctc ggccttggcg agccgagtca
     3661 tgcggctgct ggtgctctcc agccccagga ggaggttgcc cttgagctgc tccttggctc
     3721 gctggaactc ctccagatcc accgactccg tgcgcaaccg ggcgaattcg gcccggatca
     3781 ggtccaccac ctgccggtag gattcggcac tggtgccggc atacactgcc atgagccccg
     3841 aatcctgata cgaggcctga taggaataaa tggagtaggc cagaccgcgc ttctcccgga
     3901 tctcctggaa caatcgggag ctcatcgagc tgcccaggat ggcgttcagc aggaagaagg
     3961 cgtaccggct ggggtgattg tagggaagac cctcggcccc caggaccagg tggagctgcg
     4021 ccgaatcccg atcctcgtgg tggaccgtca ggtacgacac gggcgacttg ccgttggcga
     4081 cgactgcctg tccctgccag ccgccgaagg cggaccggac cagcgccgtg accttggcgt
     4141 gatccaggtc tccggccgcg gccacgatga cccgatccgg ctggtagaag tcccgcagat
     4201 gctcccggac gtcctcgcgg gtgatggcct ggagggtttg tcgcgagccg aggacgggac
     4261 ggcccagggg gtggtctcgc cagaactgct gcgtgaagag atcgtggatc aggtcgtcgg
     4321 ggtcgtcctc caccatcttg atctcttcca ggacgacgcg ccgctccttc tccagatcct
     4381 cggggtccag acgcgggtgc aggatcaggt cactcaggag gtccacggcc aacggcaggt
     4441 gctcccccag gaccttggcg taaaagcagg tgttctcccg cgaggtgaag gcgtccagcg
     4501 tcccgcccac actgtcgatg gcccgggcga tctcctcggc gctccggctc tccgttccct
     4561 tgaacaggag gtgctcgatg aagtgggaga tcccggcccg ttcggccttc tcgaaccggg
     4621 agccgacctt gacccacacg ccgatcgaca ccgaacggac ctggggcatg tgctccgtga
     4681 ggatcacgat gccgttatcg agcacctcct tctggaagtg ctcgatcacc tttccgatgg
     4741 cttcctcccc ccccgctgcc ggtcctcgcc tccggtccgc gcggggccct cggccttctc
     4801 ggccgcctct ttcaacagtt cgcggcggct caggcggatc ttccccgact tgtccacctc
     4861 gatcaccttg accatcacct cttcgccctc cctgaggacg tcctccacac gcttgacccg
     4921 ctcctgcgag atctgcgaga tgtgcagcag gccgtccgta ccaggcagga tctccacgaa
     4981 ggcgccgaag tccgtgatct tcaccacctt gcccaggtag accttcccca cctccgggac
     5041 ctcgacgatc ttcccgatga tgtccatggc cttttgggcg gccgcctcgt ccacggaggc
     5101 gatggccacg gtcccgtcgt cctcgatgtc gatcttgacc ccggtctgct ccacgatacc
     5161 gcggatgacc ttgccgccgg ggccgatcac ttccctgatc ttgtccactg ggatcttgaa
     5221 ggagataatg cggggggcgt aggggctcag cgccgtgcga ggcttggcga tggcccgatc
     5281 catgatgtcc aggacctcca tccgggccgc ctgcgcctgg gtcaaggcct ggcgcatgat
     5341 ggcctcggtg atcccctgga tcttcatgtc catctggatg gaggtgacgc ccctccgggt
     5401 gccggccacc ttgaagtcca tgtcgcccag gtgatcctcc agcccctgaa tgtcggtgag
     5461 gacaaccgcc ttgtccccct ccttcaccag ccccatggcc acgccggcca ccgccgccct
     5521 caccggaacg cccgcatcca tgagcgacag gctcgcgcca cagaccgtgg ccatggaaga
     5581 ggagccgttg gactccagga tgtcggagac gatccgcacg gtgtagggaa actcctcctt
     5641 gccggggaga acaggctgga tggcccgctc ggccagggcc ccgtggccga tgtcccgccg
     5701 gctcgccccg cgcatgaact tgacctctcc caccgagaag ggcgggaaat tatagtggag
     5761 catgaagcgc ttgaagcttt cgccctccag gtcgtccatg cgctgctcgt cctcggaggt
     5821 tccgagcgtc gtggtggcca gcgcctgggt ctgcccccgg gtgaacaggg cggagccgtg
     5881 ggtgcgtggc aggaccccga tttcgcaggt gatgggccgg acctgggtgg tcgtccggcc
     5941 gtcggcccgt cgccccttgt ccaggatcag gcgccgcatc tcctgctttt ccagctcctc
     6001 gaagatctcc ttgacctgga gttgggcggc ctcgggctcc gtcgcagcca gggccagggc
     6061 ctcgtccagg aggaccttgc gccgcgcctt ctgctcttcc ttctcgggga cgaaggccag
     6121 ctctcggatg cggcttcggc acaggtcttc cacgcgcgcc tgcagacccg cgtccaccgg
     6181 cggcgggctg aagaccggct tgttcacccc catctgtcgt gccag
//
LOCUS       DBNGZC010000797         4095 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_950586, whole genome shotgun sequence.
ACCESSION   DBNGZC010000797 DBNGZC010000000
VERSION     DBNGZC010000797.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4095)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_950586"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="AC16G0_13680"
     CDS             complement(<1..633)
                     /locus_tag="AC16G0_13680"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR5100697.1"
                     /translation="MKIVIAGAGALGSVLGGYFAQQGADVTLLARRGHVEAIHTQGLR
                     IGGRRGEQVVRNLSAVSDPAEVKAADLLILGVKSYDTRETLASLQHLRGKVGAAISVQ
                     NGGRKEEDLAEAFGQEAVVGAASLVGAAMPEPGRVLHTNDGWTWIGELDGRTTPRVQG
                     IAELFRQAKLPIEIRSDIRTAIWCKLNQMVPAAALSCVTRLFIHQLYLYRD"
     gene            902..2653
                     /locus_tag="AC16G0_13685"
     CDS             902..2653
                     /locus_tag="AC16G0_13685"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR5100698.1"
                     /translation="MKRIVISCLIVFCLYRALPVSAQAPTASPAATPSQSPAEDPLGR
                     STPRGAVLGFIKAAQEQDYARAAQYLDARQGSKLAEELARQLKLVLDRGLSAVDIDVL
                     SAKPEGNLEDALPPNQERVGKASLGSEKLDILLSRVQRGKESPIWLFSAETLRGIPQI
                     YDEVRPPWIEGFMWTPLREIRFLHYALWEWLVLLIAIPLVSGAARLLTYVLLLVLRRA
                     LYRLTGEREDRRLETISGPIRLLAVAMVIIGGTSLIGLPLLARYYWTRVAGVLIILAV
                     TWLALRLIDVVAEAVILHLQRINRANQIALARLLRRLTSATAIILAGLALLYLANVNL
                     TAALAGLGIGGLALAFAAQKTLENLFGGILIISDQPVHVGDFCRVGDYSGTVEDIGLR
                     STRIRTPSRTLVSVPNGQMAALSVENFAVRDKIWFHHTIGVRYETSADQLRYLLAEVR
                     GMLYAHPRVESDSAWVRFARFGASSLDLEIFAYVLTGDYATFLEVQEDLLLRCMEIIE
                     ASGTRIAIPSQTSYAANDPGLDAEKGRAAIARVQRWRQDRELPFPNFAPERIADLKNS
                     LEYPVAESVLRGATSQK"
     gene            complement(2747..3910)
                     /locus_tag="AC16G0_13690"
     CDS             complement(2747..3910)
                     /locus_tag="AC16G0_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016521046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HIR5100699.1"
                     /translation="MSDEIDYSFILHHLGEDEHPFNAVSPPIFQTSNFCFDSFESFQA
                     GLIAGESHYIYSRGNNPTANLVGQKIAALEKAPRARLVSSGVSAISASIMAFVQTGDH
                     VVCVKDCYSWTRKLLESYLKRFGVEHTFVEGSGPAEFEAAIRPNTRVIYLESPTTFTF
                     KLQDLASVARIARSRGIKTVIDSTWASPIFLNPIDYGIDLVVHSVSKYMGGHSDVIAG
                     AVAGSVEDIERIFATEVRQLGTSPDPLMAWLVLRGLRTLQLRMQSIFEKTLSVARFLQ
                     AHPNVEAVFYPFLESHPQYALARSQMRGGGGLFSFRLKTRDIACVKRFIDSFTLFKRA
                     VSWGGYESLVFPDAARYQSNPPPDHISLIRLYIGLEDTHALLADLERAMGRIR"
BASE COUNT          743 a         1311 c         1260 g          781 t
ORIGIN      
        1 atctcggtag aggtagagct ggtggataaa gagacgggtg acgcaggaga gcgccgctgc
       61 cggcaccatt tgattcaact tgcaccagat cgccgtccgg atgtccgacc ggatctcgat
      121 gggcaacttg gcctgccgga acagctcggc gatgccctgg acgcgcggtg tcgtccggcc
      181 gtcaagctcg ccgatccagg tccacccgtc attggtatga agcacccgcc caggttcggg
      241 cattgccgcc ccgacgaggg atgccgcccc cacaaccgcc tcctgtccaa acgcctccgc
      301 caggtcctcc tcctttcgcc cgccgttctg gaccgaaatc gccgcaccga cctttccgcg
      361 cagatgctgg agtgacgcca gggtctcccg ggtgtcatag cttttcacgc ccaggatcag
      421 gaggtccgcg gccttcacct cggccgggtc cgacacggca gagagattgc gaacgacctg
      481 ttcgcctctc ctgccgccga tgcgaagtcc ctgtgtgtga atcgcttcga cgtgcccccg
      541 cctggcgagg agcgtcacgt ccgccccttg ctgcgcgaaa taccctccga gaacgctccc
      601 cagcgccccc gctcccgcga tgacaatttt cacgcgtctt cctccgattc taagcgtatc
      661 atcggtgctg gtggcgggcg gcaggtgcct ggcggctgac ccccttcatc caccgttcgc
      721 gaagaccctg aggtttccgc tccgtgacgg gagcgaagga atctagcacg tgtatcacgt
      781 ctggtcaatt tggccaccct cggcaatcgg gcagcctgac catcgagtca gcctccccgg
      841 cggcgcggaa tcttgacctt acgaatgacc aacggcggtt cagtacgcaa cggagggttc
      901 tatgaagagg atcgtcatct cgtgcctgat cgtgttctgt ctgtatcggg ccttgccggt
      961 gtctgcacag gcgccgaccg cgtcaccggc ggcgacgccg tcccagagtc cggctgagga
     1021 tccccttggc cggagcactc ctcgaggggc ggtcctcggg ttcatcaagg cggcccagga
     1081 acaagactac gcgcgggcgg cgcagtatct ggacgcccgg caaggctcca agctggccga
     1141 agagcttgcc cggcagctca agctggtcct ggatcgcggt ctatcggcag tggatatcga
     1201 cgtgctgagc gcaaagcccg agggcaacct ggaggacgcg ctgcccccca accaggaacg
     1261 cgtggggaaa gccagtctcg ggtccgagaa gttggatatt ctccttagcc gggtccagcg
     1321 cgggaaggaa tcgccgatct ggctgttctc cgccgagacg ctccgcggca tcccacagat
     1381 ctatgatgag gtccggcccc cgtggatcga gggcttcatg tggacccccc tgcgggagat
     1441 ccgatttctg cactacgcgc tctgggagtg gcttgtgcta ctgatcgcta tcccgcttgt
     1501 gagtggagcc gcccggctgc tgacgtatgt cctattgctg gtgctccgcc gggccctcta
     1561 tcggctgacc ggcgagcggg aagaccggcg gctggaaacc atctccggtc caatccgcct
     1621 cctcgctgtg gcgatggtga tcatcggcgg gacctcgctc ataggtctcc cgctgctggc
     1681 ccgctactac tggacccgcg tggcaggcgt cctgatcatc ctggctgtga cgtggctggc
     1741 cctgcggctg atcgacgtgg tggctgaggc ggtaatcctg cacctgcaac gcatcaaccg
     1801 agcgaaccag atcgctctcg cgcgcctgct gcgacggctc acatcggcca cggcgatcat
     1861 cctggccggc ctcgccctcc tttacctcgc caacgtcaat ctgaccgccg cgctggccgg
     1921 cctcgggatc gggggtctcg ccctggcctt cgccgcccag aaaacgctgg agaacctgtt
     1981 cggcggtatc ctgatcatct ccgatcagcc ggtgcacgtc ggggacttct gtcgcgtcgg
     2041 ggactactcg gggacggtcg aggacatcgg gttgcgctcg acccgcatcc ggacgcccag
     2101 tcgaaccctg gtgagcgttc ccaatggcca gatggccgcc ctgagcgtgg agaatttcgc
     2161 ggtgcgggac aagatttggt ttcaccacac catcggggtg cgctatgaaa cctcggccga
     2221 tcagcttcgc tacctgctcg ccgaggtacg cgggatgttg tacgctcatc cccgggtaga
     2281 gtccgattcc gcgtgggtcc ggtttgcccg gttcggcgcc tcgtccctcg atctcgagat
     2341 cttcgcctac gtcctgaccg gggattacgc gacgttcctg gaagtccagg aggacctgct
     2401 gctgcgctgc atggaaatca tcgaggcgag cggaaccaga atcgccattc catcccaaac
     2461 ttcgtacgcc gccaacgatc ccgggctgga cgctgagaag ggccgggcgg caatcgccag
     2521 ggtgcagcgg tggcgacagg accgggaact ccccttcccc aatttcgccc ccgagcggat
     2581 cgcggacctg aagaatagcc tggaataccc tgtggcggag tcggtcctgc ggggagccac
     2641 aagtcagaag tagagccacc gctggctatt tttcgaaacc gagagccttc cgctctggag
     2701 ggagaagcga ggctaggagg gggacgaatc tgtcctgcgt gaggagtcag cgaattcgtc
     2761 ccatggcccg ctcgagatcg gccagaagcg cgtgcgtgtc ctccaatcca atatacagcc
     2821 ggatcagtga gatgtgatct gggggcggat tcgattgata gcgggctgcg tccgggaaca
     2881 ccaggctttc gtacccgccc cagctcacgg ccctcttgaa aagcgtgaac gagtcgatga
     2941 agcgttttac gcatgcgata tcgcgcgttt tcagacggaa cgagaatagt cccccgccac
     3001 ctcgcatctg gctgcgggcc agcgcatact gtggatgcga ttcgaggaac gggtaaaaaa
     3061 ctgcttccac gttgggatgg gcctggagga atctcgccac ggacagggtc ttttcgaaga
     3121 tgctctgcat gcgcaactgg agcgttcgca gtccccgcag caccagccac gccatcagcg
     3181 ggtccggcga ggttcccaat tgccggacct cggtggcgaa aatcctctcg atgtcctcga
     3241 cactgcccgc caccgcgccc gcgatcacat cgctatgtcc acccatgtac ttggagacgg
     3301 agtgaaccac caggtcaatc ccgtaatcga tgggattcag gaagatggga ctcgcccagg
     3361 tactgtcgat gacggtcttg atcccccggg acctggcaat ccgcgccacg ctggccaggt
     3421 cctgcagttt gaacgtgaac gtcgtggggc tttcgaggta gatgacccgc gtgttgggtc
     3481 ggatggccgc ctcgaactct gcggggccgg agccttccac aaacgtgtgc tcgacaccga
     3541 accgcttcag gtaggactcc agcagtttgc gtgtccagct gtagcagtcc ttcacgcaga
     3601 cgacatggtc gcccgtctgc acgaaagcca tgatgctcgc ggaaatcgcg gacaccccgg
     3661 aggagacaag cctcgcgcgc ggcgcctttt cgagcgcagc gatcttctgc ccgaccaagt
     3721 tggccgtcgg gttgttcccc cgagagtaga tgtagtgact ctccccggca atgagtccag
     3781 cctggaagct ctcgaacgag tcaaagcaaa agttggaagt ttggaagatc ggcggactga
     3841 cggcgttgaa cggatgctcg tcttcgccaa gatggtggag gatgaagctg tagtcgatct
     3901 cgtcggacat tctatccaac ctcgttgttg gaccacctac ccacactggg agagacaggt
     3961 cctctagccc ttgacacccc cctcggtgag accggcaatg atgaacttct gtccgatggc
     4021 gaagacgatc atcgtcggca acaaggagat cacgataccg gcgctcatga tgtcccaact
     4081 gacaccggct ttgga
//
LOCUS       DBNGZC010000798         3400 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1043847, whole genome shotgun sequence.
ACCESSION   DBNGZC010000798 DBNGZC010000000
VERSION     DBNGZC010000798.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1043847"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="AC16G0_13695"
     CDS             <1..707
                     /locus_tag="AC16G0_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5100700.1"
                     /translation="VLVPKFEKKTGYTVKTISVGTGQALALGAKGEADVCLVHSPDSE
                     KKYVADGLLLNRRLVMHNDFIIVGPAEDPAKITGLSHAVDALKRIAEAKATFVSRGDN
                     SGTHQLEKKLWAEAKIQPSGAWYLQAGQGMGATLTIASEKRAYAVTDRGTFLAFQKRV
                     QLVNLVEKDRILLNIYSVLEPNATKFPRVNAVGGKAFADFMVSKEVQEVIRTFGVEKY
                     GEPLFFPDAGKREESL"
     gene            763..1461
                     /locus_tag="AC16G0_13700"
     CDS             763..1461
                     /locus_tag="AC16G0_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5100701.1"
                     /translation="MELLWQGITQAIRLIASGDPAVLQITWLSIKISGGATLVSLLAG
                     VPAGVWLALTRFRGRGLLVALVNTGMGLPPVVVGLFVSIFLWRSGPLGFLELLYTPTA
                     IVIAQFIIAAPIVTGLTIAAMQQLNPKLRLQLLGLGASRWQILWLLMREARLPMLAAV
                     MAGFGGVISEVGASMMVGGNLLGQTRVLTTATVLETGKGDFDVAIALSVILLVLVFGV
                     NYLLTWLQQRGRAS"
     gene            1458..2549
                     /locus_tag="AC16G0_13705"
     CDS             1458..2549
                     /locus_tag="AC16G0_13705"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5100702.1"
                     /translation="MTRTILEGRDFQVAYQGTTALKVAEIRVQEGEILSLIGPNGAGK
                     STLLRVLALLEAPTSGSLLLRGHPVSWKSRDLLTLRRRLACVFQEALLCDTTVEANAA
                     LGLRLRREPSGEVEAKVRRWLDRLGIAHLTGRRARTLSGGEAQRVSLARAFVLRPEVL
                     LLDEPFAALDPPTRQELLGLLQDLLRQEGCATVFVTHDREEALRLGDRIAVMIDGRIQ
                     QIGRPPEVFGHPASEEVARFVGVETILEGRVTGRRDGLLSVDLDGARIEALGKAAVGE
                     RVLVCLRPEDLVIRQQGKPAAQESARNHLEGVIVDATRLEAQYRLQVDCGPHVIALVT
                     KESFEEMGLAPGRRVVVTFKASAVHLIRR"
     gene            2686..2898
                     /locus_tag="AC16G0_13710"
     CDS             2686..2898
                     /locus_tag="AC16G0_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015410954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HIR5100703.1"
                     /translation="MKISARNALKGRIKQIEQGAVNTEVTVELPGGIDIVSIITKKSV
                     ADLGLAKGKEVYAVIKASSVMIGVDH"
BASE COUNT          579 a         1058 c         1131 g          632 t
ORIGIN      
        1 atgtgctggt ccccaagttc gagaagaaga ccgggtacac ggtgaagacg atctcggtgg
       61 ggacggggca agcgctggcc ctgggtgcca agggcgaggc ggacgtgtgt ctggtgcatt
      121 cgccggattc cgagaagaag tacgtggcgg acgggctgct gctcaaccgg cgactggtca
      181 tgcacaacga cttcatcatc gtggggccgg ccgaggaccc tgccaagatc acgggcctgt
      241 ctcacgccgt ggacgcgctg aagcgcatcg ccgaggccaa ggccaccttc gtttcgcggg
      301 gggataactc aggcacccac cagctcgaga agaaactctg ggccgaggct aagatccaac
      361 cgtccggtgc ctggtatctc caggccggtc agggcatggg cgccacgctc acgatcgcct
      421 cggagaagcg ggcctacgcg gtgaccgacc gggggacctt cctggccttc cagaagcggg
      481 tgcagctcgt gaaccttgtg gagaaggacc gcatcctgct caacatctat tcagtcctcg
      541 aaccaaacgc gaccaagttc ccgcgggtga acgcggtggg ggggaaggcc ttcgccgact
      601 tcatggtgtc caaggaggta caggaggtca tcaggacatt cggcgtggaa aagtacggcg
      661 aaccgctgtt cttccccgac gcagggaagc gcgaggagag tctgtaggcg gcttcccgaa
      721 atgcaacacc ttcagcgaaa accgcgcagg atgtgacgga cgatggaatt gctctggcag
      781 gggatcacgc aggccatccg gctgatcgcc tcgggcgatc cggcggtcct gcagatcacc
      841 tggctctcga taaagatctc cggtggcgcc acgctggtca gtctcctggc gggggttccg
      901 gccggcgtct ggctcgccct cacccggttt cggggccgcg gtctcctggt ggcgctggtg
      961 aacacgggca tgggactccc ccccgtggtc gtgggtctct tcgtcagcat cttcctctgg
     1021 cgcagcggcc cgctgggctt tctggagctg ctctacaccc ctacggctat cgtgatcgcg
     1081 cagttcatca tcgccgcccc catcgtcacc ggcctgacga tcgccgccat gcagcagctc
     1141 aacccgaagc tccggctcca gctcctcggg ctgggtgcct cccgctggca gatcctctgg
     1201 ctcctcatgc gcgaggcgcg cctgcccatg ctggcggcgg tgatggctgg tttcggcggg
     1261 gtgatatccg aggtgggggc ctccatgatg gtgggcggaa acctgctggg acaaacccgc
     1321 gttctgacca ccgccactgt cctggagacc ggcaagggcg attttgacgt cgccatcgcc
     1381 ctcagcgtga tcttgctggt cctggttttc ggagtcaact atctgttgac ctggctccag
     1441 cagcgcgggc gagcctcgtg accaggacga ttctcgaggg ccgagacttt caggtagcgt
     1501 atcaaggcac aaccgcgctg aaggtcgccg agatcagggt gcaggagggg gagatccttt
     1561 ccctcatcgg tcccaacggg gcaggcaagt ccacgctgct ccgcgtgctg gccctcctgg
     1621 aagctccgac ctcgggcagc ctcctgttac gagggcaccc ggtctcctgg aagtctcggg
     1681 atcttctcac cctgcgccgc aggctcgcct gtgtgttcca ggaggcgctg ctctgcgata
     1741 ccacggtgga agccaatgcg gcgctggggc tgcgccttcg ccgggagccg tccggtgagg
     1801 tggaggcaaa ggtacgccgg tggctggatc ggctgggtat cgcccacctc accggccgcc
     1861 gggcccggac gctctccggc ggcgaggctc agcgggtgag cctggctcgc gccttcgtgc
     1921 tgcggccgga ggtcctgctc ctggacgagc cctttgccgc tctggatccc ccgacgcgtc
     1981 aagagttgct cggcctgctc caggacctgc tacgccagga ggggtgcgcc actgttttcg
     2041 tcacgcacga ccgggaagag gcgcttcgcc tgggcgatcg gatcgcggtc atgatcgatg
     2101 ggcgtatcca gcagatcgga cgacccccgg aggtattcgg gcatccggcc tcggaagagg
     2161 tcgcgcgctt cgtcggggtg gagacgatcc tggagggacg cgtcacgggc aggcgcgacg
     2221 ggttgttgag cgtcgatttg gacggggcca ggatcgaggc tctcgggaag gccgccgtgg
     2281 gcgaacgggt cctcgtctgc ctgcgccctg aggatttggt catccggcaa caaggcaagc
     2341 ccgcggccca ggagagtgcg cgcaatcacc tggaaggcgt catcgtggat gccacccgcc
     2401 ttgaggccca gtatcggctc caggtggact gcggccccca cgtcatcgct ctggtgacaa
     2461 aagagtcctt tgaagagatg gggctcgccc ccggtcgccg ggtggtggtg accttcaaag
     2521 cctccgcggt tcacctcatc cgacgttgag tctccaacct ctcttctcga atccttggta
     2581 gtggccttgt gcaggcgcgc ttggcggggc atactcacac cgctgaggaa atttccgtcg
     2641 ggatctggag atcccggcct gtcttccacg agaagaggct gagccatgaa gatcagcgcc
     2701 cgcaatgccc tcaaaggccg gatcaagcag atcgagcagg gagccgtcaa cacggaagtc
     2761 acggtcgaac tcccaggcgg aattgacatt gtctcaatca tcacgaagaa gtccgtcgcg
     2821 gatttgggtc tcgccaaagg caaggaggta tatgccgtca tcaaggcctc gagcgtgatg
     2881 atcggcgtcg accattgagc ccagactcgg cgaggatcgc gggggaccgc aagcggcacc
     2941 gtgcaaaagg ctccgggcat ctttttcgag cgaacccgtt gattttcctc atagaagatt
     3001 ccaattccga ccaatcagcg ccctagcccg gtgaggcgtg atgccatcgt cgcttgtgga
     3061 gccagcaggg actcacgcga atcctccttc caactgactg ctaagatagc cgcgccttcg
     3121 accgtcattg tggttggtgc ctctggtacg gggagaacgc cacagtcgtc ggtaccgtcg
     3181 ggcacaaggg tcctgatccg tcggccgcgc tgatcaaggt ccgtcaggga ggttctgggt
     3241 cgggagagaa tttcatgatc aaccaggcct ggtggtatcc cgagaacttt gtgcgccgtc
     3301 gtccggtgct ggaggttcgc gcccgcgcct tcgccggcgt gcgcgccttc ttcgccgaac
     3361 ggggcttcgt cgaggtggaa acgccttgcc tccaggtctc
//
LOCUS       DBNGZC010000799         3414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_459507, whole genome shotgun sequence.
ACCESSION   DBNGZC010000799 DBNGZC010000000
VERSION     DBNGZC010000799.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_459507"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1717
                     /locus_tag="AC16G0_13715"
     CDS             17..1717
                     /locus_tag="AC16G0_13715"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015868715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HIR5100704.1"
                     /translation="MAFDPRELERLEGERSRWERETLSDHLEKSPERTDPFSTVSGHP
                     IRRLYTPLDAPGLDVIRDLGFPGEYPFTRGVQPTMYRGRFWTMRQYSGFGSAEETNRR
                     FKYLLQQGQMGLSIAFHLPTLLGYDSDDPMPAGEVGKCGAAVDSLQDMENLLEGLPLG
                     EVTTSMTITSTAAVAWAMYIAVCEQMGIPYDRIGGTLQNDVLKEYIAQKEYIFPLKPS
                     IRLVTDTIVFGTRHLPRWNTISISGYHIREAGATALQELAFTIADGITYVEEAIRAGL
                     DVDDFAPRLSFFWDLHNDLFEEIAKLRAARRLWARLMRERFQAKNPRSWMLRTHAQTV
                     GVSLTAQQPHVNIARVALQALAGVLGGTQSLHTNSMDEAYALPTEEAVTIALRTQQMI
                     AYETGVTNTVDPLAGSYFVEALTNEMEDGAREYLRRIDDLGGMVHAIERGFPQQEIAN
                     SAYAYQRAVEQGEQVIVGVNRFADEGEKRPIPILSIDEALRERQVERVAQLRKNRDKD
                     RWSRALDALRAGAVGDDPWGRDLLMPKILQAVKAYATVGEIMGVLREAWGEYTEPNVI
                     "
     gene            1777..2184
                     /locus_tag="AC16G0_13720"
     CDS             1777..2184
                     /locus_tag="AC16G0_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HIR5100705.1"
                     /translation="MTKERKIRILIAQPGLDGHDRGAKVVARAFRDAGMEVIYTGLRQ
                     TPEMIANTAIQEDVDVIGLSCLSGAHMHLFPKVMELLKTRGADDILVFGGGTIPEEDV
                     TRLKACGLAAVYGPGSSTQDAIAFVRQNVKRRE"
     gene            2228..>3414
                     /locus_tag="AC16G0_13725"
     CDS             2228..>3414
                     /locus_tag="AC16G0_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase C-terminal domain-containing protein"
                     /protein_id="HIR5100706.1"
                     /translation="MRAVYLVSGGVSKFAKARPDVTFQPMVKEAYDYALRDLGLTTRQ
                     AAELVDGTVASYFSDHFLRQLKAGSMVQDYLGLCPKPSKRIEAGGATGGVAFQTGWES
                     VASGRYDLCVVYGWETMSHVNTWKGNEFIALASDTNFDYPVGGFYSGYYAMMVVRHMR
                     EFGTSVEQMALVSVKNHINALHNPYAQKRKRIRIEDVRQSEMVAWPLTMLDICVMSDG
                     AAVAILASDEGLNKLEKATGKRPQPVRVTGVGGGLDAMRMADRPHGEVMLLPHEKAED
                     YRGLKYPGVHSFRAGRMASKTAYQMAGIGNPLEDLDFIELHDAYTSSEIQTYEDMGLC
                     RYGEGGKFVEAGKPFLPGVDYGYRFPEQGKIPVNPSGGLIACGHPVGATGLMQAVFAL
                     WQL"
BASE COUNT          635 a         1069 c         1180 g          530 t
ORIGIN      
        1 aggcgggagg ggaatcatgg ccttcgatcc acgggaactg gagagactcg agggagagag
       61 atcccgctgg gagcgggaga ccctaagcga ccacctcgag aagagcccgg aacggaccga
      121 tccgttcagc acggtttcgg gacaccccat ccgtcgcctg tacaccccgc tggacgcccc
      181 agggctcgac gtcatccgcg atctgggttt tcccggtgag taccccttca cccggggagt
      241 ccagcccacc atgtatcggg gccgattctg gaccatgcgg cagtactccg ggttcggctc
      301 ggcagaggag accaaccggc ggttcaagta tctcctgcag caggggcaga tgggcctgtc
      361 gattgcattc cacctgccca cgctcctggg ctacgattcc gacgatccga tgcccgcggg
      421 cgaggtgggg aagtgcgggg ccgcagtgga ttccctccag gacatggaga acctgctgga
      481 ggggctgccc ctgggcgagg tcacgacgtc catgacgatc acgtccacgg cggccgtggc
      541 gtgggccatg tacatcgcgg tctgcgagca gatgggcatt ccctacgacc ggatcggcgg
      601 gacgctccag aacgatgtcc tgaaggagta catcgcccag aaggaataca tcttccccct
      661 gaagccgtcg atcagactcg tcaccgacac cattgtcttc gggacgcggc atctgccccg
      721 ctggaacacg atcagcatca gcggctacca tattcgcgag gcgggcgcca cggccctgca
      781 ggaattggcc ttcaccatcg ccgacggcat cacctacgtg gaggaggcca tccgagcggg
      841 cctcgacgtg gatgacttcg cgccgcgcct gtccttcttc tgggatctgc acaacgacct
      901 gttcgaagag atcgccaagc tccgggccgc acgacggctg tgggcgcgtc tgatgcggga
      961 gcggttccag gccaagaatc cccgctcctg gatgctccgc acccacgctc agaccgtagg
     1021 ggtgtcgctc acggcgcaac agcctcacgt gaacatcgcc cgagtggcct tgcaggcgct
     1081 ggcgggtgtc ctgggcggca cccagtcgct ccacaccaac tccatggatg aggcctacgc
     1141 cctgcccaca gaggaggccg tgaccatcgc cctccggacg cagcagatga tcgcgtacga
     1201 gacgggggtg accaacaccg ttgacccgct ggccggctcc tacttcgtgg aggcgctgac
     1261 caacgagatg gaggatgggg ccagggaata cctgcggcgc atcgacgacc tgggcgggat
     1321 ggtccatgcc atcgagcggg gcttcccgca gcaggagatc gccaactccg cctacgccta
     1381 tcagagggcg gtggagcaag gagagcaggt catcgtgggc gtcaaccgat tcgcggacga
     1441 gggggagaag cggccgatcc ccatcctcag cattgatgag gccctgcggg agcgacaggt
     1501 ggagcgcgtg gcgcagctcc ggaagaaccg ggacaaggac cggtggagcc gcgcgctgga
     1561 cgccctccgg gccggcgccg tgggagatga tccgtggggg agggatctgc tgatgccgaa
     1621 gatcctccag gcggtgaagg cgtatgcgac cgtgggcgag atcatgggtg tcctgcgaga
     1681 ggcgtgggga gagtacaccg agccgaacgt catttgacgg taggggctca gggtcaggcc
     1741 ccggcaggtg ggagccacct gacaaaggat gccagcatga cgaaggagcg aaagatccgg
     1801 atcctgatcg cacagcccgg gctggacggg cacgaccggg gcgccaaggt ggtcgcccgg
     1861 gctttccgcg acgcggggat ggaggtgatc tacaccggcc tgcgtcagac ccctgagatg
     1921 atcgccaaca ccgccatcca ggaggacgtg gacgtcatcg gcctgagctg cctctcgggc
     1981 gcgcacatgc acctgttccc gaaggtgatg gagctgctca agacgagggg cgcggatgac
     2041 atcctggtct tcggcggcgg gaccatcccg gaggaggacg tgacgaggct gaaggcttgc
     2101 ggcctggcgg cagtgtacgg gccgggctcc agcacccagg acgccatcgc cttcgtgcgc
     2161 cagaacgtca aacgcaggga atgacgcccg gctggaccgg atcggtcccc ctgaggaggg
     2221 agggatcatg cgagctgtct atctggtttc ggggggagtc agcaagtttg ccaaggcccg
     2281 acccgatgtc accttccagc cgatggtcaa ggaggcatac gactacgccc tccgcgacct
     2341 ggggctcacg acgcggcagg cggccgagct ggtggacggg acggtcgcct cgtacttctc
     2401 cgaccacttc ctgcgccaac taaaagccgg gagcatggtc caagactacc tggggctgtg
     2461 tcccaagccc agcaagcgta tcgaggcggg cggcgccacg gggggcgtcg ccttccagac
     2521 gggatgggag tcggtggcct cgggccgcta cgacctctgc gtggtttacg gctgggagac
     2581 gatgtcccac gtgaacacct ggaaggggaa cgagttcatc gccttggcct ccgacaccaa
     2641 cttcgactac ccggtgggcg gattctactc cggctactac gccatgatgg tcgtccggca
     2701 tatgcgggag ttcgggacca gcgtggagca gatggccctc gtgtcggtga agaaccacat
     2761 caacgcgctg cacaatccct acgcccagaa gcggaagcgg atccggatcg aggacgtgcg
     2821 ccaatccgag atggtggcct ggcccctgac catgctggat atctgcgtga tgagcgacgg
     2881 ggcggccgtc gccatcctag ccagcgatga ggggctgaac aagctcgaga aggcgaccgg
     2941 caagcgcccg cagcccgtac gggtgaccgg cgtgggcggc ggtctggacg ccatgcggat
     3001 ggccgaccgc ccccacgggg aggtgatgct cctcccgcac gagaaggccg aggactaccg
     3061 gggcttgaag tacccgggcg tccactcctt ccgggccggt cgtatggcct ccaagacggc
     3121 ctaccagatg gccggaatcg ggaacccgct ggaggatctg gacttcatcg agttgcacga
     3181 cgcctacacc tcctcggaga tccagaccta cgaggacatg gggctttgtc gctacggcga
     3241 aggcgggaag ttcgtggagg cggggaagcc gttcctgccc ggcgtggatt acggctaccg
     3301 cttcccggag caagggaaga ttcccgtgaa tccctccggg gggctcatcg cctgcggcca
     3361 tcccgtgggc gcgacagggc tcatgcaggc ggtcttcgcc ctgtggcagc ttca
//
LOCUS       DBNGZC010000800         4960 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_167703, whole genome shotgun sequence.
ACCESSION   DBNGZC010000800 DBNGZC010000000
VERSION     DBNGZC010000800.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_167703"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..981)
                     /locus_tag="AC16G0_13730"
     CDS             complement(160..981)
                     /locus_tag="AC16G0_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5100707.1"
                     /translation="MGSLHDFLASADQITEVEDATAAGRGTLGVRVEVQRLNKFFGTH
                     HVLRDLDLTVDPGETFVIFGPSGCGKSVLLKHIAGLLTPDSGRLVIDGEDLQAGEGRH
                     RFRMAMVFQSSALFNSLSVGENVGLWLKEHRISSRAEIAKIVAEKLALVGLTGQEKAM
                     PSELSGGMRKRVSIARALAVNPHLILYDEPTAGLDPVLTEQIGHVIVDLKKLKITSIV
                     VTHDLNLGFHVAEHLGMIHEGRLIETGTPATIRATKNPVVRAFLTTQTKILEGGG"
     gene            complement(986..1750)
                     /locus_tag="AC16G0_13735"
     CDS             complement(986..1750)
                     /locus_tag="AC16G0_13735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family ABC transporter permease"
                     /protein_id="HIR5100708.1"
                     /translation="MNTVTSRVERFLRGLGEGVTLLLQAVYWCKAAPRNMDKILAHLL
                     EFGNATLPIASLMAIFIGGVLALQTGSRLAQFGLEGNIGGIVGLSMVKELGPVMTALL
                     VAGRVGSAIAAEVGAMNVYEEIDALRTLNINPVRYLVMPRLLASLIAVPALCMYVNVI
                     GLLGGALVSAVNPKISVSMRLFRLNFQEVVRFGDVTDSLLKSVVFGVAVAITCCYVGL
                     RTTGGPEEIGKAVTRAVVLSFMFIFVLNYLITRSLM"
     gene            complement(1747..3330)
                     /locus_tag="AC16G0_13740"
     CDS             complement(1747..3330)
                     /locus_tag="AC16G0_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100709.1"
                     /translation="MTGKGERLVWLIVWLRGSGVWALCVALVLALWLAPPSAVRAAES
                     ASRPLNVWPFFDQRDDPVDRTHEQHSLGPLWGLDRAQDGSTEDLAFRPLFHRREDKTM
                     QRLEWEVLYPLMSYSRTEKDWEFQFLYLLNFRAEGSQPKEREQRADFFPFYLSGTSET
                     GETYHAVLPFGGRVLGGRIFGEDEMDFVMFPLYARFVKQGVETRYFPWPILSVTSGEQ
                     RSGFRFVPLYGHDVKAGVFEKRFVLWPIFLHQRTGLDEENPEETLTVFPFYVAQRSKP
                     RDSTTILWPFFTYTNNRERQYRQWDVFWPLIRIARGEGRTTNWFLPFYMREQRVLRRE
                     FLLKEMKTTDLGILFPIYIRSQVEFPDSLKVRDRILWWLYSDERETGRDGSTRRVDAW
                     PIFRYTRDREGRIEFQTLALLEAFMPGNEKIEQNYSPLWAVYTYRRNPEGDEVRSFLW
                     NLVRHEETSQGRSVEVLGPILAYREGEGDAHLSLLGGLLQYDVRHGVRSVRLFRDMTF
                     GWTAPSQPVAALPPTGGNR"
     gene            complement(3351..4727)
                     /gene="dnaB"
                     /locus_tag="AC16G0_13745"
     CDS             complement(3351..4727)
                     /gene="dnaB"
                     /locus_tag="AC16G0_13745"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR5100710.1"
                     /translation="MPEAASVTDRIPPQNLEAEVSVLGAVLQETGALLKAMEVLRPGD
                     FYKEAHRKIFGACVELFEKNEPVDLVTLANELMRRKHLEEIGGASYLTSLVDAVPTAA
                     NVAHHARIVKDKALLHDLIQKGTAVVGRAYADTDNVDDVLDWAEQQIFEISQHKISRS
                     FVPVKSVLKGTFQLIEKLYDRKSHVTGVPTGFRKFDEMTAGLQPSDLVVVAGRPSMGK
                     TSFCLNIAEHAAIQEHIPVAIFSLEMSKEQLVQRMLCSVASVDSHKLRTGYLSDADWP
                     KLTTGAGRLSEAPIFIDDTPGISLLEIRAKARRLMAEQGELGLVIIDYLQLISGRGRA
                     ESRQQEISEISRSLKGMAKDLNVPVMALSQLSRAVESRDDKRPQLSDLRESGAIEQDA
                     DVVAFLYRHAVYARKDPTKQQDPDEVEDNTTEVIIGKQRNGPTGTVYLAFLREFTRFE
                     DQERQREF"
     gene            complement(4755..>4960)
                     /locus_tag="AC16G0_13750"
     CDS             complement(4755..>4960)
                     /locus_tag="AC16G0_13750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bL9 family ribosomal protein"
                     /protein_id="HIR5100711.1"
                     /translation="TPLAAPKLFGSVTSQDLAEALAAQGFSLDRKKILLGEPIKTLGV
                     HRVPVRLHAEVTVEFTVIVEREA"
BASE COUNT          892 a         1631 c         1553 g          884 t
ORIGIN      
        1 ccccgatctt caagcccggg atggaccgga aggaggtctt gtactccacc gtctgcgtga
       61 agatcgtctc cttacccacc agctcgaaga gcgcgatcag gatgaggaag gctaccacca
      121 ggaatagccc gacgcgtacc tctctggaca tggccatgac tacccgcctc cttccaggat
      181 cttcgtctgg gtggtgagaa aggctcgaac caccggattc ttcgtggccc ggatggtcgc
      241 cggggttccc gtctcgatca gacgcccttc gtggatcatc cccagatgct ccgccacatg
      301 gaatccgaga ttcaagtcat gggtgaccac gatggaggtg atcttgagct tcttcaggtc
      361 cacgatcacg tggccgatct gctccgtcag cacggggtcc agaccggccg tgggctcgtc
      421 gtacaggatg aggtgaggat tgaccgccaa ggcccgggca atcgacaccc gcttgcgcat
      481 gccgcccgac agctccgagg gcatggcctt ctcctgaccg gtgaggccga ccagggccag
      541 cttctccgcc acgatcttgg cgatctcggc ccggctcgag atgcggtgct ccttcagcca
      601 gagaccgaca ttctccccga ccgagaggga gttgaagagg gccgaggact gaaacaccat
      661 ggccatgcgg aagcggtgtc gcccctcacc cgcctgcagg tcctcgccgt cgatcactaa
      721 tcggcccgaa tcgggtgtga gcagtccggc gatgtgcttc agcaggaccg acttgccgca
      781 gcccgagggg ccgaagatca cgaaggtctc ccccgggtcc acggtgaggt cgaggtctcg
      841 cagcacgtgg tgcgtgccaa agaacttgtt gaggcgttgc acctccacgc gcacgccgag
      901 cgtgccccgc cccgccgcgg tggcgtcctc gacttccgtg atctggtcgg cgctggccaa
      961 gaaatcgtgc aggctgccca taggctcaca tgagactgcg ggtgatgagg taattcaaca
     1021 cgaagatgaa catgaaggac aggacgaccg cccgggtcac cgccttgccg atctcctcgg
     1081 gcccgcccgt ggtgcggagg ccgacgtagc agcaggtgat ggccaccgcc accccgaaga
     1141 ccaccgactt gaggaggctg tccgtcacgt cgccgaatct caccacttcc tggaagttca
     1201 gacggaacaa ccgcatgctc acgctgatct tggggttcac cgcgctcacc agggcaccgc
     1261 ccagaagccc gatgacgttc acgtacatgc acagggccgg gacggcgatc aggctggcca
     1321 ggaggcgagg catcaccagg taccgcacgg ggttgatatt cagcgtcctg agggcgtcga
     1381 tctcttcgta tacgttcatc gcccccacct cggcggcgat ggccgagccg acccgccccg
     1441 caaccaagag cgcggtcatg accgggccca actccttcac catgctgagg ccgacgatgc
     1501 cgccgatgtt cccctccaga ccgaattggg ccagacgcga gccggtttgc agggccagaa
     1561 cgcccccgat gaagatcgcc atgagggagg cgatgggcag ggtggcgttg ccgaactcca
     1621 gcaggtgggc caggatcttg tccatgttcc ggggggccgc cttgcaccag tacaccgcct
     1681 gcaagaggag ggttacccct tcccccagac cccgcaggaa ccgctcgact cgtgaggtca
     1741 cggtattcac cggttccctc ctgtcggggg cagggcggcg accggctgcg atggcgcggt
     1801 ccacccgaag gtcatgtccc ggaacaggcg caccgatcgg acgccgtgtc gcacgtcata
     1861 ctgcagcagt ccgccgagca gggagaggtg ggcgtcccct tccccttccc ggtaggccag
     1921 tatcggtccc aggacttcga cggagcgccc ctggctcgtc tcctcgtgac gcaccaggtt
     1981 ccagaggaag gatcgaacct catcgccctc gggattccgc cgataggtgt acacggccca
     2041 gagcggggaa tagttctgct cgatcttctc attccccggc atgaacgctt ccagaagggc
     2101 caaggtctgg aactcgatcc gtccctcccg gtctcgggta tagcggaata tcggccaggc
     2161 atccacccgc cgggtggagc cgtcccggcc tgtctcccgc tcgtcggaat acagccacca
     2221 gaggatgcga tcgcgcacct tgaggctgtc ggggaactcc acttggctgc gaatgtagat
     2281 cgggaacagg atgccgagat cggtagtctt catctccttc agcaggaatt cacgccgcag
     2341 cacccgttgc tcgcgcatgt agaaggggag gaaccaattg gtggtgcgtc cttctccccg
     2401 ggcgatccgg atcagcggcc agaacacgtc ccactgccgg tactgacgct cccggttgtt
     2461 ggtgtaggtg aagaacggcc acaggatcgt ggtgctatcc cgaggctttg agcgctgcgc
     2521 aacatagaag ggaaacacgg tcagcgtctc ttccgggttc tcctcgtcca ggccggttcg
     2581 ctggtggagg aagatcggcc agagcacgaa tcgcttctca aagacccctg ctttcacatc
     2641 gtgaccgtac agggggacga agcgaaaccc cgagcgctgc tctccgctgg tgaccgagag
     2701 tatcggccag gggaagtacc gggtctccac accctgcttt acgaagcggg cgtaaagggg
     2761 gaacatgacg aagtccattt cgtcctcgcc gaagattcgc ccgcccagga cgcgtccgcc
     2821 aaagggcagg accgcgtggt acgtctcgcc agtttcgctg gtcccgctca ggtaaaaggg
     2881 gaagaagtcg gcacgctgct cccgttcctt cggctgggag ccctccgccc ggaaattcaa
     2941 aagatacagg aactggaact cccagtcctt ttccgtccgg ctgtatgaca tgaggggata
     3001 cagcacctcc cactccaggc gctgcatcgt cttgtcctcc cgccggtgga acagcggccg
     3061 gaaggccaga tcttcggtgc tgccatcctg ggcccggtcc aacccccaga gcggccccag
     3121 gctatgctgc tcgtgcgtgc ggtccaccgg atcatcccgc tggtcgaaga atggccagac
     3181 gttcagcgga cgggaggcgg attcggcggc ccgcaccgcg gagggcgggg ccagccacag
     3241 ggccagaacg agagcaacgc acagcgccca gactcccgag cctctcagcc acacgatcag
     3301 ccacacgagc cgttccccct tgcccgtcat ccgagcctcc gccttgccgt ctagaattcg
     3361 cgctgccgct cctggtcctc gaaccgggta aactcccgca ggaatgccag gtagaccgtc
     3421 cccgtgggtc cgtttcgctg cttcccgatg atcacctcgg tcgtattgtc ctccacctcg
     3481 tccggatcct gctgcttggt ggggtccttg cgcgcgtaca ccgcgtgacg atagagaaag
     3541 gcgacgacat ccgcatcctg ctcgatggca cccgactcgc ggaggtccga gagttgcggc
     3601 cgcttgtcgt cgcgcgactc gaccgcgcgg gagagctggg acagggccat caccgggacg
     3661 ttcagatcct tggccatccc cttgagggag cgagagatct cggagatctc ttgctgccgg
     3721 ctctcggctc gcccccgacc cgagatgagc tgcaggtagt cgatgatcac cagacccaac
     3781 tcgccctgct ccgccatcag gcgccgcgcc ttggcgcgaa tctccaggag agagatgccg
     3841 ggcgtgtcat cgatgaagat gggggcttcg gacagccgac ccgccccggt ggtgagcttc
     3901 ggccagtcgg catcggacag gtacccggtg cgcagcttgt gcgagtccac actggccacg
     3961 gagcagagca tgcgctggac caactgctct ttggacatct cgaggctgaa gatggcgaca
     4021 gggatgtgct cttggatggc ggcgtgctcg gcgatgttca ggcagaagct ggtcttcccc
     4081 atggaggggc ggcccgccac caccaccagg tccgacggct gcagccccgc ggtcatctcg
     4141 tcgaacttcc ggaaccctgt gggcaccccg gtgacgtggc tctttcgatc gtacagcttc
     4201 tcgatgagct ggaaggtgcc tttgagaacg ctcttcaccg ggacgaagga ccggctgatc
     4261 ttgtgctggg agatttcgaa tatctgctgc tcggcccagt ccaggacatc gtccacgttg
     4321 tccgtgtcgg cataggcccg gcccaccacc gccgtgccct tctggatcag atcgtgcaag
     4381 agggccttgt ccttgacgat gcgggcgtgg tgggcgacgt tggccgcggt cggcacggca
     4441 tccaccagcg aggtcaggta gctggccccg ccgatctcct cgagatgctt ccggcgcatg
     4501 agctcgttgg ccagggtcac caggtccacg ggctcgtttt tctcgaagag ctctacgcag
     4561 gcaccaaaga tcttccggtg ggcttccttg tagaaatcgc cgggccggag aacctccatg
     4621 gccttcagga gggcccccgt ctcttgcagc accgccccca ggacggaaac ctccgcctcg
     4681 aggttctggg gggggattct gtcggttacg ctagccgcct caggcatgga cgtggtcgga
     4741 cagacgtcgc acccctaagc ctcgcgctct acgatgacgg tgaactcgac tgtcacctcg
     4801 gcgtgcagac gcaccggcac ccggtgcaca ccgagggttt tgatgggctc gcccagcaga
     4861 atcttttttc tgtccaggct gaacccctgg gccgccaggg cctcggcgag gtcctggctg
     4921 gtcacggatc cgaacagctt gggcgcggcc aggggcgtcg
//
LOCUS       DBNGZC010000801         2052 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2221483, whole genome shotgun sequence.
ACCESSION   DBNGZC010000801 DBNGZC010000000
VERSION     DBNGZC010000801.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2221483"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="AC16G0_13755"
     CDS             <1..709
                     /locus_tag="AC16G0_13755"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006636 - unsaturated fatty acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HIR5100712.1"
                     /translation="GIGICMTYHRLLTHRSFATRPRWLEYALTAIGCCASEGGAIGWV
                     ADHRKHHAHSDDEHDVHSPNRGFAWAHMLWWMTPDITSRHTPQYYKKWAPDLYNDPVH
                     RWLDGNQILFPILLFVTLYAFGGMSWLVWGGFVRTILVLHTTWLVNSATHVWGYRSHE
                     TRDTSTNLWWVALLTYGEGWHNNHHAFQTSARHGLRWWEVDMTYVAIRLMAVVGLAHS
                     VKRPKTATAARPGPPIS"
     gene            821..1252
                     /locus_tag="AC16G0_13760"
     CDS             821..1252
                     /locus_tag="AC16G0_13760"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: protein motif:HMM:NF015370.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - stearoyl-[ACP] desaturase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HIR5100713.1"
                     /translation="MDLIESLEGFVAGNLGLLAPVDHAWQPTDFLPDLTADDWAEQLT
                     RFRASARRLSNDLVVVLVGSMVTEEALPSYAVSLNRIVKDQTGTGETPWARWLRGWTA
                     EENRHGDLLNAYLRLRGRVDMRAVERTIHHLIAKGFNTGIE"
     gene            complement(1382..>2052)
                     /locus_tag="AC16G0_13765"
     CDS             complement(1382..>2052)
                     /locus_tag="AC16G0_13765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding response regulator"
                     /protein_id="HIR5100714.1"
                     /translation="IAPEMLPKVFDLFTQMDRSLDRADGGLGIGLTLVRRLTEMHGGT
                     VAAMSAGQGQGSEFVVRLPALPDKAPTDRPTLPAPAKRRVAPASGRRILVVDDNRDSA
                     DSMAMLLRLFGNDVRTVQDGRLALEVAAAYRPSVVLLDIGLPGLDGLEVCRRLRARND
                     ESQPQIVALTGYGMEEDRRRCQEAGFDSHMVKPVDLETLQEMLSRPEIEGNRPKSERE
                     GLAP"
BASE COUNT          349 a          718 c          636 g          349 t
ORIGIN      
        1 cggcatcggc atctgcatga cctaccaccg cctcctgacc caccgcagct tcgcgactcg
       61 gccccggtgg ctcgaatacg ctctgacggc catcggctgc tgcgcctccg aagggggggc
      121 gatcggctgg gtggccgatc accgtaagca ccacgcccac tccgacgacg agcacgacgt
      181 tcatagcccc aaccgcggct tcgcctgggc ccatatgctc tggtggatga cccccgatat
      241 cacctcgcgc cacacccccc aatactacaa gaaatgggcc cctgatctgt acaacgatcc
      301 cgttcaccgg tggctcgacg gtaatcagat cctgtttccc atcctgttgt tcgtgacgct
      361 ctacgccttc ggtgggatga gttggctggt ctggggcgga ttcgtccgga cgatccttgt
      421 gctccatacc acctggctgg tcaactcggc cacccatgtc tggggttatc gcagccacga
      481 gacgcgcgac acctcgacga acctctggtg ggtcgccctg ctgacctacg gcgagggctg
      541 gcacaacaac caccacgcct tccagacctc ggcccgccat ggcctgcggt ggtgggaagt
      601 ggatatgacc tacgtggcca tccgcctcat ggccgtggtc gggctggccc acagcgtgaa
      661 gcgccccaag acggccaccg cggcgcggcc cgggcccccc atctcctgaa atcgcgacgg
      721 agctgatggg ggcgtaccgc tgagccgcgc accgtcccgt acgaccgtca tgcggtgtgc
      781 aaaacccgcg caccgcagct atcgaccggg gacccccgac atggatctga tcgagagctt
      841 ggagggattc gtcgcaggaa acctgggact gcttgcgccc gtggatcatg cctggcagcc
      901 gaccgacttc ctccccgacc tgaccgcgga cgattgggcc gagcagctca cgcggttccg
      961 ggcttccgcg cggcgcctgt ccaacgactt ggtggtcgtc ctggtgggga gcatggtcac
     1021 ggaagaggcc ttgccgagtt acgccgtctc gctcaatcgg atcgtcaagg accagaccgg
     1081 gaccggcgag accccctggg cccgatggtt gcgggggtgg accgccgagg agaaccggca
     1141 cggcgacctg ctcaacgcct acctccgcct cagaggccgt gtggacatgc gtgcggtcga
     1201 gcggacgatc catcacctca tcgcaaaggg attcaacacg gggatcgagt agcaacggag
     1261 cagaaaccaa ccaccagccg tccagttagc tggacggccc agggatgggc cgtttctcac
     1321 cacttccgct cgaactcatt ctgaagctgc cggcgaccga ccctcccaaa gcagagcgga
     1381 gtcacggagc taacccctcg cgttcactct ttggtcgatt gccctctatc tccggtcggg
     1441 acaacatctc ttggagtgtt tccaggtcaa ccggcttcac catgtggctg tcgaagccgg
     1501 cttcctggca gcgccggcgg tcctcctcca tgccgtagcc cgtcaaggcc acgatctgcg
     1561 gctggctttc gtcgttccgc gcacgcaggc ggcgacagac ctctagccca tccagaccgg
     1621 gtaggccgat gtcgaggagc accacgctgg ggcggtaggc cgccgccact tccagggcca
     1681 gcctgccgtc ctggaccgtg cgcacgtcgt tgccgaacag cctcagcagc atcgccatgc
     1741 tgtccgcgga gtcccggtta tcgtccacca ccaagatgcg tcgaccggac gccggtgcaa
     1801 cgcggcgttt cgccggcgct ggtagggtcg gtcggtcggt cggggccttg tccggcagcg
     1861 cgggcaggcg cacgacgaac tcgctaccct ggccttggcc tgcgctcatg gccgccaccg
     1921 tgccaccgtg catctccgtg agccggcgca ccagggtcag gccgatgccc aagccgccat
     1981 cggcgcggtc gagagaacgg tccatctggg tgaagaggtc aaacaccttg ggcaacatct
     2041 ccggggcgat gc
//
LOCUS       DBNGZC010000802         1616 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2124515, whole genome shotgun sequence.
ACCESSION   DBNGZC010000802 DBNGZC010000000
VERSION     DBNGZC010000802.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2124515"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="AC16G0_13770"
     CDS             complement(<1..734)
                     /locus_tag="AC16G0_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_819545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose 1,6-bisphosphatase"
                     /protein_id="HIR5100715.1"
                     /translation="MEITLSVIKADVGSIGGHTRPSARMLDSVRARLREAIRAELVLD
                     GTVTYTGDDIAIIMSHRRGLGAPEVHHFAWDSFLAANKAARTGGLYGAGQDLLVDAPS
                     GNLRGAGPAVAEIEFDRNPASKDRPAEAFLVFAADKCGPGAYGYPLYSVFCDPMHNGG
                     LLLSPKLHQGFTLTIIDMDHKSDGGDRVIRLDVPERVWDAACLLQNPDRFAIEAIHSR
                     FKPDEQIVSVSATRLHNIAGKYTGKD"
     gene            1206..>1616
                     /locus_tag="AC16G0_13775"
     CDS             1206..>1616
                     /locus_tag="AC16G0_13775"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF015999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HIR5100716.1"
                     /translation="MLKRFEEFLGSHGVTYERIEHREAFTAQEEAAAAGVSGWSWAKV
                     VIVKDREGLAMAVLPACCEVEMDRLKGVIGLGEIELASVEDILKTFPGCELGAVPPFG
                     RLFAVPTIVEEAVVNQRDVIMPAGDHRSAVRMRAT"
BASE COUNT          279 a          519 c          546 g          272 t
ORIGIN      
        1 tcgtccttgc cggtgtactt gccggcgatg ttgtgcaggc gcgtcgccga caccgacacg
       61 atctgctcgt ccggcttgaa ccgggaatga atggcctcga tggcgaagcg atcggggttc
      121 tggaggaggc aggctgcatc ccacacgcgt tcggggacgt cgagcctgat cacgcggtcc
      181 ccgccgtcgc tcttgtggtc catatcgatg atggtcagcg tgaacccctg gtgaagcttc
      241 gggctcagca ggagcccgcc gttgtgcatg ggatcgcaga agaccgagta cagcggatag
      301 ccgtaggccc cgggaccgca cttgtcggcg gcgaacacca ggaaggcctc ggcagggcga
      361 tccttgctcg caggattccg atcgaactcg atctcggcca cggcgggccc ggcgccgcgc
      421 aggttgcctg agggggcatc cacgaggagg tcctgaccgg ccccgtagag gccgcccgtt
      481 ctcgccgcct tgttagccgc caggaagctg tcccaggcga agtgatgtac ctccggagca
      541 cccagtccgc gccggtggct catgatgatc gcgatgtcgt caccggtgta ggtgacggtt
      601 ccgtcgagca cgagctcggc acggatcgct tcccgcagcc tcgcgcgcac cgagtcgagc
      661 atgcgcgcgg atggcctggt atgtccgccg atggaaccga cgtcggcctt gatgacggag
      721 agcgtgatct ccatggcagg tctccttccg gtgtcgaagc gagtgtgaac tgcccagggg
      781 gctccgaaga gccttgactc ggccctgacc agaccgtctc ctttctacgc ccgggcgatg
      841 tcaaggggca agatgcaaac tgagcggttt cgtgatggat gcgaccagcg atcctggaaa
      901 tccagccccc tgctctcgag cttcggtctg aagcgacccc atttggggca actccgccac
      961 actggggcca catggcctgt cgcctgcggc cgcgaagccc ttgaattcta tggcccggta
     1021 agtctggcct cctggcccgc agattgctcc agaagaaggg cactcccgct ggccatcctg
     1081 aacggcaagg agacgtatcg gtcggggcag ccatgcgcta gagatcgccc gcggccagac
     1141 cctgccgagc gctatcctgc cgcgcccgcc gaggcccaag cggtgcttca acgagggagg
     1201 acacgatgct caaacggttc gaagaattcc tgggaagcca cggcgtgacg tacgagcgga
     1261 tcgagcaccg ggaggccttc acagcccagg aggaagcggc ggctgccggc gtctccggct
     1321 ggtcatgggc gaaagtggtg atcgtcaagg accgcgaggg gctggccatg gccgtgctcc
     1381 ccgcatgctg cgaggtcgag atggaccggc tcaagggcgt gatcggcctc ggcgagatcg
     1441 aactcgccag cgtcgaggac atcctcaaga ccttccccgg gtgcgaactc ggggccgtcc
     1501 cgcccttcgg gaggctcttc gcggttccga cgatcgtgga ggaggcagtc gtcaaccagc
     1561 gggacgtcat catgcccgcc ggagaccatc gaagcgctgt ccgcatgcgc gcgacc
//
LOCUS       DBNGZC010000803         2558 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2614115, whole genome shotgun sequence.
ACCESSION   DBNGZC010000803 DBNGZC010000000
VERSION     DBNGZC010000803.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2614115"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="AC16G0_13780"
     CDS             <1..342
                     /locus_tag="AC16G0_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR5100717.1"
                     /translation="MTLVGIFVLICGLFIDTLPAVIILVPVLAPVADQVGIDPLHFAM
                     AFVLNITIGMVTPPVGAVLFIITAVGQLNYVKLSRAIVPMVFALLVVLVIVMYIPAIS
                     LTLPRWFGFAR"
     gene            369..1382
                     /locus_tag="AC16G0_13785"
     CDS             369..1382
                     /locus_tag="AC16G0_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR5100718.1"
                     /translation="MKWRSGYLVALAIALLAFPSAALAQQKVLKYAHFQPARSDQPKH
                     AAALAFKEHVEKATNGSIKVEIFPAGQFGNDADTMSGLRLGTLEMAVAHDGAIAGIYK
                     PIGILGIPFLYDNHEHAWRVYDSPWLKEFSDDMVKKSGVRLLSLADNGVRHFTNSKRP
                     IRTPDDMKGLKIRVMPSPVFQTLVTALGASPSAIPWAELPTALQQKVVDGEENGVTNI
                     IAASLYQYQKYITLDGHVWSIHGYTINEKFYQSLTPVERKAVEEGTQIATGIHRKMTS
                     DQDKNAKEILGKLGMEVTEITPAELAQFRKLAQPPVKAWAEKEIGKNYVDSLFKTIEA
                     TRR"
     gene            complement(1894..>2558)
                     /locus_tag="AC16G0_13790"
     CDS             complement(1894..>2558)
                     /locus_tag="AC16G0_13790"
                     /inference="COORDINATES: protein motif:HMM:NF014841.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HIR5100719.1"
                     /translation="PGYGATAVAEYTLGLILALTRHIAASSYSFKHGEWVPAKFQGVD
                     LKGKILGIVGLGPIGVDMARLGAGIGMNLIGWTRNATQDRAQHGLRLVPLEEVFSSAD
                     VVTIHLALNAQTERLITRELLSRMKPTAYFINTARAKIVDNQALADLLQQGRIAGAAL
                     DVHEDEPAPMNYRFASLPNVLVTPHVGYNSKEAGQNMLRIAYATLEAFLRGEKLHVVN
                     SV"
BASE COUNT          497 a          833 c          777 g          451 t
ORIGIN      
        1 atgacgctcg tcgggatatt cgtgctgatc tgcgggctgt tcatcgacac cctccccgcc
       61 gtgatcatcc tggtgcccgt cctggctccg gtggcggacc aggttgggat cgatccgctg
      121 cacttcgcca tggccttcgt gctcaacatc accataggca tggtaacgcc cccggtcggc
      181 gccgtcctct tcatcatcac ggccgtcggc cagctcaact acgtgaaact ctcgcgggcc
      241 atcgtgccca tggtgttcgc actcctggtg gttctggtaa ttgtcatgta catcccggcg
      301 atcagcttga cactgcccag atggttcggg tttgctcgtt aaccctgatg gaagaaggag
      361 gaagggccat gaagtggaga agcggttatc tcgtcgccct ggccatcgcg ctgctggctt
      421 tcccctcggc cgccctggcc cagcagaagg ttctgaagta cgcccacttc caacccgcca
      481 ggtccgacca gccgaagcat gccgccgcgc ttgccttcaa ggagcacgtc gagaaggcga
      541 ccaacggctc gatcaaggtt gagatcttcc cggcgggcca gttcggcaac gacgctgaca
      601 ccatgtcggg actccggctc ggcaccctgg agatggccgt ggcacacgac ggcgccatcg
      661 ccgggatcta caaacccatt ggcatcctcg gcatcccctt cttgtacgac aaccacgagc
      721 acgcctggcg ggtgtacgac agtccctggc tgaaggagtt ctccgacgac atggtcaaga
      781 agtccggggt ccggctcctg tcgctcgccg acaacggcgt gcgccacttc accaacagca
      841 agcggcccat ccgcacccca gacgacatga aggggctcaa gatccgcgtg atgcccagcc
      901 ccgtcttcca gaccctcgtc accgccctcg gcgccagccc ctcggcgatc ccgtgggcgg
      961 aactgccgac ggcgctccag cagaaggtcg tggatgggga agagaatggc gtgacgaata
     1021 tcatcgccgc cagcctgtac cagtaccaaa agtacatcac gctggacggc catgtctgga
     1081 gcatccacgg ctacacgatc aacgagaagt tctaccaaag cctcacgcct gtcgagcgga
     1141 aggccgtgga ggagggcacg cagatagcca cgggcatcca ccgcaagatg accagtgatc
     1201 aggacaagaa cgccaaggag atcctcggca agctcggcat ggaggtcacc gagatcacgc
     1261 ctgccgagct ggcgcagttc cgcaagctgg cccagccgcc ggtcaaggcc tgggcggaga
     1321 aggagatcgg caagaactac gtggacagcc tcttcaagac catcgaggcg acgcgcaggt
     1381 aacgaccccg cggcccctcc cacgcactga tactggggac cccccggctc gggaccatcg
     1441 cgaggccccg agccgaggga ccccgcctgc tattccgaca tcatagaacg cataccccgc
     1501 ccatcgcgtc gcatagcctc ttgagccccc aggacttggg gagcctgctt gccgaaatcg
     1561 gcgacagccg gtgatctccg cagttcagcc aaatggtcga gataggcctt tcgcacaact
     1621 ggagtgcccg ggggacggac cccctgcgga gacgagggtc ccaatgctta atatgaggca
     1681 ctggtgagaa gtaatcacat tagctcggat acgaggtacg aaagtaccgc agatgaggtt
     1741 cttgcgggtc acagcagatt cgtcgcccct atcctgccta atcctctccg tccccggccg
     1801 ccgcaggctg ctacgtgaga cacctcggcg accgtcgagc ctcatgactg attctcgggg
     1861 acctggacgg gctgaatagt agcccagcga cgctcacacg gaattcacga catggagttt
     1921 ctctcccctc aagaacgcct cgagggtggc gtaggcgatt cgcagcatgt tctgcccggc
     1981 ctctttcgag ttgtacccga catgtggcgt gaccagcacg ttggggaggg aggcgaagcg
     2041 ataattcatg ggggccggct catcctcatg gacgtcgaga gccgcgcccg caatgcggcc
     2101 ttgctggagc aggtctgcaa gggcctggtt gtcgacgatc ttcgcccggg ccgtgttgat
     2161 gaaatacgcc gtcggcttca ttctgctgag gagctcgcgg gtgatgaggc gctcggtctg
     2221 ggcattcagt gccagatgaa tcgtcaccac gtccgcggag gagaagacct cctccagggg
     2281 gacgaggcgc aggccgtgct gggcgcgatc ctgcgtggcg ttccgcgtcc agccaatcag
     2341 gttcatccca atgccggccc cgagccgcgc catgtcgacc ccaatggggc cgagcccgac
     2401 gataccgagg atcttgcctt tgaggtctac gccttggaac ttggcgggca cccattcgcc
     2461 gtgtttgaag ctgtaagagg acgcggcaat gtgccgggtg agggctagga tgagtcccaa
     2521 cgtgtactcc gccaccgccg tcgcaccgta ccccggcg
//
LOCUS       DBNGZC010000804         5469 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_557526, whole genome shotgun sequence.
ACCESSION   DBNGZC010000804 DBNGZC010000000
VERSION     DBNGZC010000804.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5469)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5469)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5469
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_557526"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(622..3246)
                     /locus_tag="AC16G0_13795"
     CDS             complement(622..3246)
                     /locus_tag="AC16G0_13795"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR5100720.1"
                     /translation="MSAPVTNRDFRLPRAVVPTRYQIRLEPDLTTFTFAGTETVEVTV
                     RQAVTEIVLNAVELQIRSVSLQDSRGKTLHGTAVLDEAAERLTLRFSQSLSPGPCRLS
                     LEFTGILNDKLHGFYRSTVRPPASPSLSGLTGKGDAGSVLAVTQFEATEARRAFPCWD
                     EPDFKAVFEVTLVVDPDLRAISNTPVQSETPVRGTGKKAVRFAPTIKMSTYLLAFVVG
                     RLEAIEGRPLGGTPIRIWVVAGKQHLGVFAQEVAAFCLRFFEDYYAISYPGDKLDLIA
                     IPDFAFGAMENLGAITFRETALLVDEKTATHGELERVAEVVAHEIAHMWFGDLVTMAW
                     WNGLWLNEAFATFMEMLAVDAWRPEWARWVAFGVSRAAALLVDGLRSSRPIEFEVVAP
                     KDAEAMFDVLTYEKGGAVLRMLEQYLGPPVFRDGVRKYLADHQYGNAETTDLWKALGE
                     VSGQPTPSIMDGWIFRPGYPMVTVRLEDGGRTLHFSQHRFTYLRDESPPSDLWQVPIN
                     YRVQLSGEVKNLSLLLSSAEDRVELPDRPDWVVVNEGGHGFYRVRHTGDLQAKLAENP
                     SGILAPIERFNLINDVWASVLAGLAPGREYLDLTGRFREEDDRNVWTALLGSLVYLQR
                     VIAPRSRPALEALVRDRLGPAVARLGWTPAPGEGELTRQLRGELLRAMGTLGNDPAVQ
                     ATAREISLRCQDDPSAVDPNVVPAVIAVLAHCGNAADYEVFLERFKTAKTPQEEQRYL
                     YSLAGFRVPELLRRTLDFTLSGEVRSQDAPFLVRSLLMNVHAREMAWTFVKENWEVME
                     HRYPSLSGLRRMCEGITGLTTPELEADVRQFFASRNVALGGKTLEQYLEQLHIAVVFA
                     GRDGVDLEGYLGESLWAL"
     gene            complement(3423..3746)
                     /locus_tag="AC16G0_13800"
     CDS             complement(3423..3746)
                     /locus_tag="AC16G0_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100721.1"
                     /translation="MTVLLGVALWLLANQAQAEEVIFLQDGRTITADKTEVIGDRLRV
                     VKPTETFDIPRESVLTIHKVAPPQDSPNATPPAEVYRDVTQQMNEKVRQDTQKLLNPP
                     RVFPH"
     gene            complement(3990..4295)
                     /locus_tag="AC16G0_13805"
     CDS             complement(3990..4295)
                     /locus_tag="AC16G0_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100722.1"
                     /translation="MASGGRREETDARIKQWEQELERVRLALARAPEEVHARWNPGFA
                     NLYRRKEVVKSAWEAIRGIYRPEPEALQRFEQAWRAMEADWAAAQPMLAEVPACEAA"
     gene            4600..5277
                     /locus_tag="AC16G0_13810"
     CDS             4600..5277
                     /locus_tag="AC16G0_13810"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013010631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR5100723.1"
                     /translation="MRGRYEINQLEKEESWRMFRIIGELVEGFDALSRITPAITVFGS
                     AKARPGEFAYDMAEAIGREIARADFTVVTGGGPGVMEAACKGAVEAGGKAVGLNIELP
                     VEQKPNPYLTLSLNFHHFFVRKVMLVKYATAFVLLPGGFGTLDELFETVTLIQTHKIR
                     PFPMILVGWDYWRGLIDWLHDQAVAQGHLDASDLDFLTITDSPAEVVGHITRFYEGQS
                     MDLSAPE"
BASE COUNT         1040 a         1812 c         1629 g          988 t
ORIGIN      
        1 acctgcgctg tcaccgccgc gtccgccgct gtcgcttccg ccaccgccgc caccactgcc
       61 actcccacta cctccgccgg atccaccgtc gctaccgccg ccgccgccac tgccgccacc
      121 acccccatcg ccgccgccgt ctttcgcata ggcaattccg gtgtccttgt ggctaaatgg
      181 atgctgcagg tcggccaggt acggcacggt caccagcgcg ataattagaa cagtgagcca
      241 accgccaagt cttcgcatca tatccagttc ctccttcacc atccctcaac tagatcctgt
      301 cgattctacc gtcaaactcc ctcacacctc gaaactacca ggcagggttc aatgaaagta
      361 ctcagcaagg cgaatgccaa agataacggt caggagcaac ctgcgcatga agcctacaaa
      421 gcccacaagc ccaaggatat acgatgcttc caggtaaata gctcgcgata gcttatcggc
      481 tgtattcgtt gcacggggcg ggaggaatgt ggatttgccc atgcgtgggc atatgcccaa
      541 cggtcaaaac ctgattcaat tgtcggacct tagtccgcga ggtaacccat gtcttatctt
      601 ccgggcacca aagggtacgg ctcaaagagc ccagagggat tcccctagat acccctcgag
      661 atccaccccg tcacgcccgg cgaacaccac cgcgatgtgg agttgctcca ggtactgctc
      721 caacgtcttc ccccccaacg cgacgttcct ggaggcaaag aattgccgca cgtccgcttc
      781 cagttccggc gtagtgagcc ccgtgatccc ctcgcacatt cgcctgagtc cactcaggga
      841 agggtaccgg tgctccatga cttcccagtt ctccttgacg aaggtccagg ccatctcccg
      901 ggcatggacg ttcatcagca gactccgaac cagaaacggc gcatcctggc tgcgcacttc
      961 gccgctcagc gtgaaatcaa gcgtccggcg cagcagctcc ggcacgcgga atccggccag
     1021 ggagtagaga taccgctgtt cctcttgcgg cgtcttggcc gtcttgaaac gctccaggaa
     1081 cacctcgtag tcggccgcat tgccgcaatg agcgaggacc gcgatcacgg ccggcacaac
     1141 gttcggatcc accgcggaag gatcatcctg gcagcgaagg gaaatctccc ttgccgtcgc
     1201 ctggacggct gggtcgttcc caagggtccc catggcccgc aacagttcac cacgaagctg
     1261 gcgtgtcagc tccccttcgc ccggcgcggg ggtccagccg agccgggcca ccgccggtcc
     1321 aagccggtca cgcacgagag cctccaacgc cggccgactt ctcggggcga taacccgctg
     1381 caggtagacc agcgacccca gaagtgcggt ccacacattc cggtcgtcct cctcccggaa
     1441 ccgcccggtc aggtccaagt actcacgccc aggggcgagc ccggcgagga ccgaggccca
     1501 gacgtcgttg atgagattga accgctcgat gggcgcaagg atcccggacg gattctcggc
     1561 aagtttcgcc tggaggtccc cggtgtgacg cacccggtag aatccgtgcc cgccctcatt
     1621 tacgaccacc cagtctggcc gatcgggcag ctccacacga tcctccgccg atgacaggag
     1681 gaggcttagg ttcttcacct cgccgctcaa ttggacccga tagttgattg gaacctgcca
     1741 aaggtccgag ggaggtgatt cgtcgcggag gtaagtgaag cggtgctggg aaaagtggag
     1801 cgtcctccct ccgtcttcca gcctgaccgt caccatgggg taaccggggc ggaagatcca
     1861 cccatccatg atgctggggg tgggttgacc ggagacctcc ccgagcgcct tccacaggtc
     1921 ggtcgtctcg gcattgccgt actggtgatc ggcgaggtac ttccggaccc cgtcgcggaa
     1981 gaccgggggg ccgagatact gctccagcat ccggagcacc gcccccccct tctcataggt
     2041 caggacgtcg aacatggcct cggcgtcctt gggggcgacc acctcgaact cgatgggacg
     2101 actcgatcgg agcccgtcca ccaagagggc tgcagcccgc gagacgccga aggcgaccca
     2161 gcgcgcccac tccggcctcc aggcgtccac ggccagcatc tccatgaacg tggcgaaggc
     2221 ctcgttgagc cacaggccgt tccaccaggc catggtcacc agatcgccga accacatgtg
     2281 ggcaatctcg tgggccacga cctccgccac ccgttccagc tcgccatggg tggccgtctt
     2341 ctcatccacg aggagagcgg tctcgcgaaa cgtgatggcc cccagattct ccatcgctcc
     2401 gaaggcaaaa tcagggatcg caatcaagtc cagcttgtcg ccgggatagg aaatggcgta
     2461 gtaatcctcg aagaagcgca ggcagaaggc cgcgacctct tgcgcaaata caccgaggtg
     2521 ctgcttgccg gccacgaccc agatccggat cggtgtgccg cccagcggtc tcccctcgat
     2581 ggcctccagc cttccgacga cgaaagccag caagtaggtg gacatcttga tagtcggggc
     2641 gaagcgaacc gccttcttgc ccgtccctcg gacgggggtc tcgctctgga cgggtgtatt
     2701 cgagatcgcg cgcaggtccg ggtcgacgac caaggtcacc tcgaacacgg ccttgaaatc
     2761 aggctcgtcc cagcacggga aggcgcgacg cgcctccgtg gcctcgaact gggtgaccgc
     2821 tagcacggag cccgcatcgc ctttgcctgt cagcccggag agcgacggcg atgcaggtgg
     2881 cctgacggtg ctccggtaga agccgtggag tttgtcgttc aagatcccgg tgaactcgag
     2941 ggacagccgg caggggccgg gactgaggct ctgggagaag cgcagcgtga ggcgctctgc
     3001 cgcctcgtca agcaccgcgg tcccgtggag tgtcttgcct cggctgtcct ggagactgac
     3061 gctgcgtatc tgcagctcga ctgcattcag cacaatctct gttacggcct gccgaacggt
     3121 cacctcgacc gtctctgtgc ccgcaaaggt aaatgtcgtc agatccggct cgagccggat
     3181 ctggtagcgg gtggggacca cggcgcgcgg cagcctgaag tctcggttcg tcaccggagc
     3241 gctcaatcga cgatcctcct tccgtcgcca ggcaggaatg gactccctcg atacccacgg
     3301 gtttcgaggg gctcaaatat ccttcctcaa cgaggattcg tcaattccgg gaatcaggga
     3361 gaggaaccac agatcgtgga gaggccaaaa gctgaccctg gaaagctggt cgttttccag
     3421 attcagtgag gaaacacccg gggagggttg agcaacttct gagtgtcctg gcgaaccttc
     3481 tcgttcatct gctgcgtcac gtcgcggtag acctctgcgg gcggggtcgc attaggagaa
     3541 tcctggggcg gagcgacctt gtggatcgtg aggacgcttt cccgcgggat gtcgaaggtc
     3601 tcggtgggct tcacgacacg gagacgatcg ccgatgacct ccgtcttgtc cgccgtgatg
     3661 gtccgcccgt cctgcagaaa gatgacctcc tcggcctgcg cttggttagc aaggagccag
     3721 agggcgaccc ccagcaggac agtcatcaga actacacctg acaatcggtg agaagttgag
     3781 ccaaccatcg agccaccttc aatgagatcg accttgaatg aaagtatact cctcagagac
     3841 tccacgcgca cgagctaaat ccgtcgcgct cctttcgcca ggcacccccc accggcgtcg
     3901 gtagccccgg agaactcacg gctgcgctca agcccaatcc gcgccaggct acggcgagcc
     3961 atgctccgcc tggtgggtgc ccaatgccct catgccgcct cgcaggccgg gacctcggcc
     4021 agcatcggct gggcggccgc ccagtccgct tccatggccc tccaggcttg ctcgaagcgc
     4081 tgcagggctt cgggctccgg gcgatagatg ccacggattg cctcccaggc agatttcacg
     4141 acctccttcc ggcggtagag attggcgaag ccaggattcc acctggcatg cacctcctcg
     4201 ggtgcccggg ccagggcgag gcgcacccgc tcgagttcct gctcccactg cttgatccgt
     4261 gcgtctgttt cctcacgcct gccaccggat gccattctcg tcctctccac tcaatcctgc
     4321 gatcgcctcg aaatgagacc ccctgcctct ccgggaacgc atctgatcta tagcacgtct
     4381 ggcattcccg cgtcgaggcc aggctgccgc tgccccgtga tccacacgac gccaacgtcg
     4441 aagaggcgta gaatcccttg cagggtttcc cgccctagtc taccctaacg cggggtggcg
     4501 tccagctcga tccaaacgga tgggagcggg gagtcctgtc accctcggat gctggtctgg
     4561 cgccttcgtc caccacgggc ttctcacgag gagagggaca tgcggggccg ctacgagatc
     4621 aatcagctgg agaaagaaga atcctggcgg atgttccgaa tcatcggcga gctcgtcgag
     4681 ggtttcgatg ccctctcccg catcacgccg gccattaccg ttttcggcag cgccaaagcc
     4741 cggccgggag agtttgccta cgacatggca gaggccatcg gccgcgagat cgccagagcc
     4801 gacttcaccg tggtcactgg cgggggcccc ggcgtgatgg aggcggcgtg caagggtgcc
     4861 gtggaagcgg gaggcaaggc ggtcggtctg aacatcgagc tgccggtgga gcagaagccc
     4921 aacccctatc tcaccctctc actgaatttc catcacttct tcgtgcgaaa ggtgatgctg
     4981 gtgaagtacg cgactgcctt cgtcctgctg cctggggggt tcggaactct ggatgagctg
     5041 ttcgagacag tcaccctcat ccagacccac aagatccgcc cattcccgat gatcctggtg
     5101 gggtgggatt attggcgcgg tctcatcgat tggctgcacg atcaagcggt cgcccagggc
     5161 cacctcgacg cgtctgacct agatttcctg acgatcacgg acagccctgc cgaggttgtg
     5221 ggccacatca cccggttcta cgagggccag agcatggact tgagcgctcc ggagtgaccc
     5281 ggatgccggg ctgggtggac gacggtccgg gacattggag gcactctcac cgagccccga
     5341 gcctcgacct ctggccgcga tcattcgacc ctgacgacga cttgggccgg aagtcgttgg
     5401 aggaggcact ggagggcttg ctcgacgtac gtggtatcct tggattccag cgtcagcatc
     5461 acgttatgg
//
LOCUS       DBNGZC010000805         1986 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2322424, whole genome shotgun sequence.
ACCESSION   DBNGZC010000805 DBNGZC010000000
VERSION     DBNGZC010000805.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2322424"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1986
                     /locus_tag="AC16G0_13815"
     CDS             <1..>1986
                     /locus_tag="AC16G0_13815"
                     /inference="COORDINATES: protein motif:HMM:NF012901.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HIR5100724.1"
                     /translation="EMFHGRGIEPDYVIGHSVGEIVAAYVAGMLDLEDAARFVVARGR
                     LMGELPPGGKMLAIAASVEQVERWVEGRESEVAIATVNGPRSVVISGTAEAVAAVGEL
                     AQAAGRQTKELEVSHAFHSPLMDPVLDDLARVSASMHMSPARIPIVSNMTGDFFGDEV
                     SPDYWSAHMRHAVLFHQGMEKIIGAGCSLLVEIGPHPALTPAVVTAFDSASLQTVPTL
                     RRDKKDAANILGSVASLYVGGVTLKLDRLYSDPAHHRVSLPLYPFRREKYSLDPGGFL
                     DMGPEGFASMGVGAAPQELPALPEPHPLLGKVVTRNPRKTVFETAFKPVSPWTDHRVL
                     DTTVFPATGFMEMAARGFAATSGTDWRAVVLKDVTFEQPLLLSYEQEKNVGLVLDGPA
                     NPAGSTRFAIAASGDGASPYCRGRIAPAGDGQDRVDMNQELGGRKSELSIGPFYGELR
                     MRGLEYGARFANVRELWQGEPGSGEAFGRVSITSPDGETDKDPYQGAVLLDGCLHVFG
                     AALGALDNPGQPGTFVPASVQSVTLRRELPAQVWSHVKASVGADGRAALANVRILNDE
                     GELIAEFEKLELRRALSLAPAKRNGAAGEGGAKTSADQIFRSREHALQLLGPLSRKDR
                     IALLSKWLAAEIMDTMGQAGEGLNLDKLPPSTAFLEIG"
BASE COUNT          337 a          623 c          642 g          384 t
ORIGIN      
        1 gagatgtttc acggtagggg aatcgagccg gattatgtca tcggtcatag cgtcggcgag
       61 atcgtcgctg cttatgtggc cggcatgctg gatctggagg acgctgcccg ttttgtggtc
      121 gctcgtggcc gcttgatggg cgagcttccc ccgggcggga aaatgctggc catcgccgca
      181 tccgtggaac aggtggagag gtgggtcgag ggccgcgaaa gcgaggtagc catcgccacc
      241 gtcaacggcc cacgctctgt ggtcatctct ggcaccgctg aagccgtggc cgctgtcggc
      301 gaattggccc aggctgcggg acggcagacg aaggagctcg aggtatcaca tgccttccac
      361 tcgcccctca tggatcccgt cttggatgac ctggcgcgcg tttcggcttc catgcacatg
      421 tcccccgcca gaatcccgat agtttccaac atgaccggcg atttcttcgg cgacgaggtc
      481 agccccgatt actggagtgc ccatatgcgg cacgccgtgc tcttccacca gggcatggag
      541 aagataatcg gtgccggatg ctctctcctg gtcgagattg ggcctcatcc ggcgctgaca
      601 ccggccgttg tcaccgcctt tgattcagct tcacttcaaa cggtgccgac ccttcggcgg
      661 gacaagaagg atgcggccaa tatccttgga agcgtggctt cgctgtatgt agggggtgtg
      721 accctgaagc tggaccggct ttattcggat cccgcccacc accgtgtctc cttgcccctt
      781 tatcccttcc gccgggaaaa atactcgctg gatccgggag ggttccttga tatggggcct
      841 gaaggcttcg catccatggg cgtgggtgcc gccccccagg agctgcccgc gctacccgag
      901 cctcacccat tgctcggcaa ggtcgtgacc cgcaacccgc gcaagaccgt cttcgaaaca
      961 gccttcaagc cggtcagccc ctggaccgat catcgcgttc ttgatacgac ggtcttcccc
     1021 gctaccggat tcatggagat ggctgcgcgc ggtttcgcgg ccacctctgg gaccgactgg
     1081 cgcgcggtgg tgctcaagga tgtgaccttc gaacaacccc tgctgctttc ctacgagcaa
     1141 gaaaagaatg ttggcctggt cctcgatggg cccgctaacc ctgcgggcag cacccggttt
     1201 gccatcgcag ccagcgggga cggtgcttcg ccctattgcc gtggccgcat cgctccggcc
     1261 ggtgatgggc aggatcgggt cgatatgaac caggagctcg gcggccgcaa gtcagaattg
     1321 agcattggcc ccttctacgg cgagctgcgc atgcgcgggc tcgagtacgg tgcaaggttc
     1381 gccaacgtac gcgagctctg gcagggggag ccgggctccg gggaagcttt tggccgggtc
     1441 agcatcacat cgccggatgg cgagacagat aaagatccct accaaggcgc cgtcctactg
     1501 gacggatgcc tgcacgtctt tggggcggcc ctgggcgcgc tcgataaccc cggccagccc
     1561 ggcaccttcg tcccggcctc ggtccagtcc gtgaccctgc ggcgcgagct gcccgcgcag
     1621 gtctggagcc atgtgaaagc cagcgtcggg gcagatgggc gggccgctct cgccaacgtg
     1681 cgaatcctga acgacgaggg tgagctgatt gctgaattcg agaagctgga gcttcgccgc
     1741 gccctctccc tcgcgcccgc gaagcgcaat ggcgcagcgg gtgagggcgg cgcgaaaacc
     1801 tctgcggatc agatttttag gtcccgggag catgctctgc agctcctggg cccgctctca
     1861 aggaaggatc gtatcgcgct cctgtcgaag tggctcgcgg ccgagattat ggacactatg
     1921 gggcaggccg gcgaggggct gaatctggac aaactcccgc cctcgaccgc gtttctagag
     1981 atcggg
//
LOCUS       DBNGZC010000806         6506 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_366553, whole genome shotgun sequence.
ACCESSION   DBNGZC010000806 DBNGZC010000000
VERSION     DBNGZC010000806.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6506)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6506)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6506
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_366553"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1683
                     /gene="arc"
                     /locus_tag="AC16G0_13820"
     CDS             <1..1683
                     /gene="arc"
                     /locus_tag="AC16G0_13820"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HIR5100725.1"
                     /translation="QVQALEEEMGHLRRRLEQTPKEFEFLRTKLAQAREQLEQAHQQN
                     ERLVGALAQAKEQLTALRAEVDKLAAPPSSYGTVLQPHAEGTVDVWTAGRKLKVSVAP
                     SVPAASLQRGQEVVLNEALNVIALAGPEPQGEVVRLKDRLDATRLVVTLRADEERVVE
                     LAAPLTATPLQVGDHLRLDARAGLVLEKLPKAEVEDLVLEEVPNITYQDIGGLGAQIE
                     LIRDAIELPVLYAEHFAAHKLAPPKGVLLYGPPGCGKTLIAKAVANALAQQLSTQTGT
                     PSTAYFLNVKGPELLNKYVGETERKIREIFARAREKANAGCPVVIFFDEMDSLFRTRG
                     SGISSDVESTIVPQFLAELDGVEGLRHVIVIGASNRQDLIDPAVLRPGRFDVKIRIDR
                     PTQEAAQEIFGKYLTPDLPLAAGELAAHGADPAATATALIAAALAEMFVADEAHRFLE
                     VTYASGEKETLYFKDFVSGALIESVCTRAKKTAVKRMITSGARGLTTADLVEAVKAEF
                     RENEDLPNTTNPDDWAKIAGRRGERIVHVRPVFDREGKKPRKVETISTGHYL"
     gene            2288..4180
                     /locus_tag="AC16G0_13825"
     CDS             2288..4180
                     /locus_tag="AC16G0_13825"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR5100726.1"
                     /translation="MTPRPANNPWIRELDRLRWLGIWLPVTAIAVLVVLAEGVLLYLD
                     LPVWVTVGAHALMLGVITLGAYLFSTYIFRIVRGQEEEILQKRQELAARERHFRALIE
                     NSSDGIVLLNATGVYTYASPSTTRMLGYPPEELVGRSAFEIIHPDDRERAMARTIESL
                     RNPGAIIRAEVRIRHRDGSWRWIEAVVSNLLADPSVQAIVKNYRDVTQRKRDEEALRS
                     AHAELEERVRERTADLVRAHESLQSTLEQQWRAEEALRESQAQLAGIIRSAIDAIITI
                     DDQHRIVVFNAAAERLFRVSSAEVIGQSVQQFIPERFRSTHEAGLRDFARTNLETWWV
                     GMLGGVAGLRRGGQEFPFEAAVSQVEVGGRKLYTVILHDTTAQKEAEAQLRDSQEQLR
                     ALSARLLTVREEERARIAQEIHNTVGQALAVLKMDLAWVASRLPDGQTPLAERSKAML
                     ALINGTVHSVRRIATELRPAVLDDLGLVAALEWQAKEFQTRMSIACEFTSSQSDLAVT
                     PAAGTAVFRICQEALTNVARHARATRVGISLKEEEENLVLTVADNGRGITDQEIANRT
                     SLGLLGMQERALHLGGNVNIAGRSGKGTTLTVRIPLTAPTFSDAREESREQDRMLTRP
                     VDPNPR"
     gene            5527..6150
                     /locus_tag="AC16G0_13830"
     CDS             5527..6150
                     /locus_tag="AC16G0_13830"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR5100727.1"
                     /translation="MAIGGKTTELGASGMAPEITVNSRATEFGVSQRLPAVSDPVYDH
                     FLQGFRPLHADARRRSYLVAEVVTAANQNRIDPDLLFALVAVESNFNSTAVSPKGARG
                     LGQIMFATGRMVAPRIVREPEDLYSVPRNLHVTALYLRRLLIERNGDLRTALAAYRTG
                     AAVGHLLGRDDTVYVDRICRHFAFLKTKREYREMTAMAVGSAAARRD"
     gene            complement(6262..>6506)
                     /locus_tag="AC16G0_13835"
     CDS             complement(6262..>6506)
                     /locus_tag="AC16G0_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HIR5100728.1"
                     /translation="GAVRKTTEGLFPKGWAAKTGIFPLSDLRPAPAYEKIVEAHGGYG
                     ERVEKPERVRPALQRALRAVKEEHRQAVLNVICKHD"
BASE COUNT         1261 a         2095 c         1986 g         1164 t
ORIGIN      
        1 caggtgcagg ccctggagga ggagatgggc cacctgcgcc gccgcctgga gcagaccccg
       61 aaggagttcg agttcctccg gaccaagctg gcccaggccc gggagcagct ggagcaggcc
      121 caccagcaga acgagcgcct ggtgggcgcc ctggcccagg ccaaggagca gctcacggcc
      181 ctccgggccg aggtggacaa gctggcggcc cccccctcga gctacgggac cgtcctccag
      241 ccccacgccg aggggacggt ggacgtctgg acggccggcc ggaagctcaa ggtgagcgtc
      301 gcccccagcg tcccggcggc cagcctccag cggggccagg aggtcgtctt gaacgaggcc
      361 ctgaacgtga tcgccctggc cggccccgag ccccagggcg aggtggtccg gctgaaggac
      421 cgcctggacg ccacgcgcct cgtggtgacg ctccgggccg acgaggagcg ggtggtggag
      481 ctggccgccc ccctcaccgc cacccccctc caggtggggg atcacctccg cctggacgcc
      541 cgggcgggcc tggtcctgga gaagctcccc aaggccgagg tggaggacct ggtcctggag
      601 gaggtcccga acatcaccta ccaggacatc gggggcctcg gggcccagat cgagctcatc
      661 cgggacgcca tcgagctccc ggtgctctac gccgagcact tcgccgccca caagctcgcc
      721 ccgcccaagg gggtcctgct ctacggcccc ccgggctgcg gcaagaccct catcgccaag
      781 gccgtggcca acgccctggc ccagcagctc agcacccaga ccggcacccc cagcacggcc
      841 tacttcctga acgtcaaggg ccccgagctc ctgaacaagt acgtggggga gacggagcgg
      901 aagatccggg agatcttcgc ccgggcgcgg gagaaggcca acgccggctg cccggtggtc
      961 atcttcttcg acgagatgga ctccctcttc cggacccggg gctccggcat ctcctcggac
     1021 gtcgagtcca ccatcgtccc ccagttcctg gcggagctgg acggggtgga ggggctccgc
     1081 cacgtcatcg tcatcggggc gagcaaccgc caggacctga tcgaccccgc ggtcctccgg
     1141 ccgggccgct tcgacgtgaa gatccgcatc gaccggccga cccaggaggc cgcccaggag
     1201 atcttcggga agtacctcac ccccgacctc cccctggccg ccggggagct cgccgcccac
     1261 ggggccgacc ccgcggccac cgccaccgcc ctgatcgccg ccgccctggc cgagatgttc
     1321 gtcgcggatg aggcccaccg cttcctggag gtcacctacg cctccgggga gaaggagacc
     1381 ctctacttca aggacttcgt gtcgggcgcc ctcatcgaga gcgtctgcac ccgggccaag
     1441 aagaccgcgg tgaagcgcat gatcaccagc ggggcccggg gcctcaccac ggccgacctg
     1501 gtggaggccg tgaaggccga gttccgcgag aacgaggacc tccccaacac caccaacccc
     1561 gacgactggg cgaagatcgc ggggcgccgc ggcgagcgca tcgtgcacgt ccggccggtc
     1621 ttcgaccgcg agggcaagaa gccccgcaag gtcgagacca tcagcacggg acactacctc
     1681 tgagcgcccg gtccgggccc ccccgccgcc tgcctccccg gatccgtgcc ctccgtgtgc
     1741 ctgggtcaag ggatcccgct ggcaaaggac tgcctgcggc atcgcgaacg accaccgcaa
     1801 ccgccctctc aattctgcac gccacgcgcc cctgacttgt cctacagact gcacgcgccg
     1861 aaacatgtcc attcacattc cgttcacacc aggaggcacc ctcaaagcga ggcccgcaag
     1921 cctcgctcat tcccgaaaaa cgccagctcg tttttcgctt tccaaagcgt gctgagcggc
     1981 tgatggtggg catttgcccg cgaagggcaa atgcccacgg aatatcttcg agtgatgtaa
     2041 gaggtcctat gcctgatatc gcgtccccta tcgcgaaatt tcgtgttaat ccgttctcca
     2101 ctgcggggtt ccccgtgctc tcacacgcgg gagctccctc acgcgttagg caggcttcgc
     2161 ggcacctgtc ttgcactgta taatttcgga ttcgccatca tttggggact actacggttt
     2221 agggaattgt cgagatgctt atcgcaactc tgagaaattg aacccgcgag tgacaggatt
     2281 gatcgccatg accccacgac ctgcaaacaa cccctggata cgtgaactcg acaggctgag
     2341 atggctcggc atctggttgc ccgtgaccgc cattgcagtt ctggtcgtcc ttgcagaggg
     2401 cgtgctcctc tacttggatc tgcctgtatg ggtgacggtc ggagcccatg cgcttatgct
     2461 gggggtgatc accctgggag cctatctgtt ctccacgtac atcttccgga tcgtccgggg
     2521 tcaggaggaa gaaatcctcc agaagcggca ggagctggcg gcccgggaac gacatttccg
     2581 agcgctcatc gagaacagct ctgacggcat cgtgctgctc aacgccacgg gcgtctacac
     2641 gtacgctagc ccctcgacga cccggatgct cgggtacccg ccggaggagt tggttggtcg
     2701 cagcgcgttt gaaatcatcc atcccgacga ccgtgaacgc gccatggctc gcacgatcga
     2761 atcacttcga aacccgggag cgattatccg agccgaagtc cgcatccggc acagggacgg
     2821 ttcctggaga tggattgaag cggttgtcag caacttgctc gccgatccaa gcgtccaggc
     2881 gattgtcaag aactaccggg atgttaccca gcgcaagcga gatgaggagg cgctgcgcag
     2941 cgcacatgcc gagctggagg agcgcgtgcg agaacggacc gccgacctcg tccgcgccca
     3001 cgagtccctg caatcgaccc tcgagcagca atggcgtgcc gaggaggccc tacgcgaaag
     3061 tcaggcgcag ctcgccggga tcatccggtc tgccatcgac gccatcatca ccatagatga
     3121 ccagcatcgt attgtggtgt tcaacgccgc agccgagcga ttgttccggg tttcctcggc
     3181 cgaggtgatc ggacaatcag tgcagcaatt tatcccggag cgttttcgct caacgcacga
     3241 ggccggcctc cgcgactttg cacggacaaa tctcgagaca tggtgggtcg ggatgctggg
     3301 cggcgtggcg gggctccgcc gcggcggtca ggagtttccc ttcgaggcgg cggtttccca
     3361 agtggaggtg gggggaagga agctgtacac cgtcatcctg catgatacca ccgcacagaa
     3421 ggaggcagag gcgcaattgc gggactccca ggagcagttg cgtgccctgt cggcccgcct
     3481 gctgaccgtg cgcgaggagg aacgagcgcg gatcgcccag gagatccata acacggtggg
     3541 gcaggctttg gcggtcctga agatggacct cgcctgggtt gcctcgagat tgccagacgg
     3601 ccagacgccc ctggcggagc ggtccaaagc gatgctggcg ctcatcaacg gcactgttca
     3661 ttcggttcgt cggattgcga cggaactccg cccagcggtt ctggatgatc tcggactggt
     3721 ggccgccctc gagtggcaag ccaaggaatt ccagacacgt atgagtatcg cctgcgaatt
     3781 cacctctagc cagtcggacc tggccgtgac tccagcagcc ggcacggcgg tcttccgaat
     3841 ttgccaggag gcactgacga atgtggcccg tcatgcccgc gccacgcgag tcggtataag
     3901 cctaaaagag gaggaggaga acctggtgct gacggtggcc gataatgggc gaggtatcac
     3961 cgaccaagag atcgccaatc ggacatcgct cgggctcctt ggaatgcaag agcgggcgct
     4021 gcacttgggc gggaatgtca acatagcggg acggtcgggg aaaggcacca cgctaacggt
     4081 gcgtatcccg ctgacggcac ccacctttag cgatgccagg gaggaatctc gggagcagga
     4141 tcgtatgctg actcgacctg ttgatcccaa tcctcgatga gccacttcgc ggaaagagat
     4201 tcttggctcg ttcccgtcac cggggaccgc cgcttcacga gctgctcgac accgctccat
     4261 tccgtcctcc cctttcccct tggctctcgc gtgaacacga ctggaaggcg gaaaagctaa
     4321 ggggtccgag accgtcgcag caggcggctt ggggacccga catgaacccc tcggttcgtg
     4381 taggagaaac accgaccttc ctttgggctg gtatctgacg gcaattaccg ccggagtcct
     4441 attccattca cgagacttaa ggagtatctt cgcgaaatgg atcgcaagtg tgacctcaga
     4501 aagagaacga ccaggaggag ggtcaatgtc gctccaaacc cgaactgaat tcaagtacga
     4561 cctcgattcg atcacccaga acgctccgcc cgcttctggg gtcttcatga tctattcttg
     4621 ggatgcttgt gtgtttgtcg gggaatccga cgacatctgc gccagcctgt tggaaatcta
     4681 ttatgaggcg aatccttgcc tggccaagaa acacctcacg cactttagct ttgagttggc
     4741 cccgcctgag gcccgcggca cgctgcaatg cgagtgcatc ggagagctcg gaccagcatg
     4801 taatctcggg cagggattac cagagtgcag ggattgccac ctcaggcaat ggcaagggca
     4861 gggaagtgta gtgcgggtac cagctccatt ctagccagag agccgcgagc ctgcgcaccg
     4921 atctggccga gtcattccct gtgcatggga tgctactcgt tgaagacagc aggaccagac
     4981 tgcgcgtcat tgaaatcgta tgaagccctt ttcaacactc ttgcctcaaa gtgctagcct
     5041 ccagaggatc cctgtcattt gtccgccaaa tgaaacctgc catccaatag tttcccggta
     5101 attcttacac agcagcgggc cgaggtcgcc tgcgccccat cctcctcaac aaggtttgtc
     5161 cccgttcatt ttcaactgct tacgcgagaa aaccaatgcg gacagactct tcggcacgga
     5221 gtttgccctt ttcgccatgg acaccaggca gtcccgaaca tcatttactc cagatccaac
     5281 acgccacagc aatcggattt atctgtcgca tcctctgact tgggaccaca gggcttggtc
     5341 tttccagtgg tgcacgaaca gggatcactc ctcggggagg attctataga ccagggaggg
     5401 ggacccatgc cgaagtgcag tgtacagaga attcagggtt tgctatctag cctgaggacc
     5461 gtcggccccc ggcaacgaca ccgaaggggg cggggaaggt tcacgcttat tacgatcctc
     5521 ctgctgatgg ccatcggcgg aaagacgacg gagcttggag cttccggaat ggcgcctgaa
     5581 atcaccgtta actcccgtgc cactgaattt ggggtgtcgc aaagactgcc ggcagtttct
     5641 gatccggtgt atgaccactt tctccaagga ttcaggccgc tccatgccga cgctcgacgc
     5701 cgcagctatc tcgtggccga agtggtgaca gccgcgaacc agaatcggat agaccccgac
     5761 ctgctctttg ctctggttgc ggtggaatcg aatttcaaca gcacggccgt ctcacccaag
     5821 ggggcgcgcg gtcttggcca gatcatgttc gccaccggaa ggatggtggc gcccaggatc
     5881 gtgcgcgagc ccgaggatct ctacagcgtc ccccggaatc tccatgtcac ggcgctctat
     5941 cttcgacggc tcttgatcga gcgaaacgga gatcttcgga ctgcacttgc cgcctaccgt
     6001 actggcgctg ccgttgggca cttgctcggg cgagacgaca cggtatacgt ggatcgcatc
     6061 tgtaggcact ttgccttcct gaagacgaaa cgagaatacc gcgagatgac ggccatggca
     6121 gtcggaagtg ctgcggcgcg ccgagactga ataacaaggg atgacgctca ccactcgagg
     6181 acacttgtct aattctcacg gacacacccc atcaaatagc ttgcagcgcg cgtgactcgc
     6241 tgaactgagg ggaaatttcc gtcagtcgtg tttgcagatg acgttgagga ccgcttgccg
     6301 gtgttcttct ttcaccgctc gcaacgcgcg ttgtagggca ggccgcaccc gttcgggttt
     6361 ctcgactcgc tcgccatagc ctccatgggc ttcgacgatc ttctcgtagg ctggagccgg
     6421 cctcagatcg ctgagaggga agatcccggt cttcgcggcc catcccttcg gaaaaagccc
     6481 ctccgttgtc ttccggacgg cgcccc
//
LOCUS       DBNGZC010000807         3938 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1140656, whole genome shotgun sequence.
ACCESSION   DBNGZC010000807 DBNGZC010000000
VERSION     DBNGZC010000807.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1140656"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /gene="cofD"
                     /locus_tag="AC16G0_13840"
     CDS             complement(<1..862)
                     /gene="cofD"
                     /locus_tag="AC16G0_13840"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: protein motif:HMM:TIGR01819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HIR5100729.1"
                     /translation="MRIVVLAGGVGGSKFLWGLAQEVGQDDLTAIVNTGDDIELHGLQ
                     VSPDVDIVTYTLAGLVDAEKGWGFAGDTFHCLQVLKTYGQPAWFNLGDRDLATHIFRT
                     QALRQKRRPTEVADAIRRALGVQATILPMTDDPVGTHILVGDGPVRSLHFQEYLVQRG
                     AQDQVRGVEYRGSARAHPSPEVVAALDGAEAIFIAPSNPIASIGPILAVPGIRDRLRD
                     AKVPVVAVSPVVGGASLKGPTDKFLQWAKVEVSPLGVSRFYLSVLGRLDGMLIDRADA
                     ALAPAMEAEGV"
     gene            complement(1013..1678)
                     /gene="npdG"
                     /locus_tag="AC16G0_13845"
     CDS             complement(1013..1678)
                     /gene="npdG"
                     /locus_tag="AC16G0_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0006740 - NADPH regeneration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HIR5100730.1"
                     /translation="MRIAVVGGTGKEGQGLALGWARAGQELIIGSRALERAAQTAEAI
                     NREVGRAAATGMLNRQAAITGEIVVLTVPYDAHEKTLLDIREAVRGKILVDVTVPVDP
                     ERPRRLRTPIGGSATEEAQALLGSETRVVAAFQNLSYTHLAQGQAVASDVLVCGDDLQ
                     SRQEVIKLAGLLRLRALDAGPARNARVVEGLTVVLMEINRQYKVRGAGIRVTGLPEGI
                     NGK"
     gene            complement(1760..2935)
                     /gene="cofH"
                     /locus_tag="AC16G0_13850"
     CDS             complement(1760..2935)
                     /gene="cofH"
                     /locus_tag="AC16G0_13850"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013172791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HIR5100731.1"
                     /translation="MGLPCAAGSAVGRVPVSVARILDRALEGRELTREEGVILAELPP
                     DMVGPLTAVADEVRRSRVGDIITYVVNRNINFTNVCIIGCKFCAFSTGPNASDAYDLT
                     LEQIAVKAAEAWERGATEVCIQGGLPRDLDGYHYRDILRAVKRATPKMHIHAFSPMEV
                     VYGVEKTGMPLADYLAMLKDEGLGSLPGTAAEILDDGVRQLLSRLKVTVKQWVEVITT
                     AHRLGIPTTSTMMYGHVEEPAHWVNQLCLLRDIQRQTGGFTEFVPLGFIHLNTNVFRE
                     GKTRPGPTLEEHLKVHALARLMLQGAIDNIQISWVKLGRELSQRCLLAGANDYSGTLM
                     EENISRLAGATAGEYLSPEEMQERIWELDRIPAERSTTYKLLRIFPADGKSQMTNDK"
     gene            complement(3003..>3938)
                     /gene="cofG"
                     /locus_tag="AC16G0_13855"
     CDS             complement(3003..>3938)
                     /gene="cofG"
                     /locus_tag="AC16G0_13855"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF004884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HIR5100732.1"
                     /translation="VVEEGARLGCTEALFSLGDAPEAVFPEARAILQGRGYRRTLDYL
                     RAACALVLTRSPLFPHANAGLMGEPDLRALKPLNASMGLMLESASPRLLLPGGPHHGA
                     VTKHPRARLHVLEMAGELRIPFTTGLLIGIGETPEERVDSLLAIRVLHERYGHVQEVI
                     IQNFRAKARIPMHDQPEPTLEEMLRTTAVARLLLGAYMNLQAPPNLTQDYGRLLDAGI
                     NDWGGVSPVTADFINPEAPWPHLAALRAATETRGFLLRQRLPVYPEYVLDRPAFLPPE
                     LAERIRACVDGDGLVPEDRNARPATDGSTEPAEIT"
BASE COUNT          662 a         1356 c         1242 g          678 t
ORIGIN      
        1 ggacgccctc ggcttccatg gccggcgcga gtgcggcgtc tgcccggtcg atcagcattc
       61 catccaacct gcccaggacc gacaggtaga accggctcac tccgagcggc gacacctcga
      121 ccttggccca ctggaggaac ttatccgtgg gaccctttaa cgaggcgccg cccaccacgg
      181 ggctcacggc aacgacgggc accttggcgt cccgcaggcg atcgcggatc ccgggaacgg
      241 ccaggattgg gccgatgctg gcgatgggat tgctgggcgc gatgaagatc gcctccgcac
      301 catccagggc ggccacgacc tcggggctgg gatgggcgcg ggcgctcccc cggtactcga
      361 ccccccggac ctggtcctgc gcgccacgct gtaccaggta ctcctggaag tgcagggagc
      421 gcaccggccc gtcgccgacc aggatgtgcg tcccgacggg atcgtccgtc atcggcagga
      481 tcgtcgcctg gacccccagc gcgcgccgga tggcgtccgc cacctccgtt ggccgacgct
      541 tctgtcgcag cgcctgcgtg cggaagatgt gggtcgccag atcccggtca cccaggttga
      601 accaggcagg ctgaccgtat gttttcagga cctgcaggca gtgaaacgtg tccccggcga
      661 acccccagcc cttctccgca tccacgaggc cggcgagagt gtaggtcacg atatccacgt
      721 cgggtgagac ttgcaggccg tgcagctcga tgtcgtcgcc cgtgttgacg atggcggtga
      781 gatcgtcctg gcccacctcc tgcgccagcc cccagaggaa cttggagccc ccgacccctc
      841 cggccagcac cacgattcgc atcgacacat cgcctcgctt caatgaaaaa gggaaaaccg
      901 aaaatccgtg cctattgaaa acgcacaacc gacagtgccg atgcaaaacc gacaacggac
      961 aactgacaac cgatcaattc cgcacattga ccggttgtcc gttgtcagtt tctcattttc
     1021 cattgatgcc ttcgggaagg cccgtcaccc ggatcccggc gccccgaacc ttgtactgtc
     1081 ggttgatctc catcaggaca accgtcaggc cttctacgac gcgcgcgttg cgcgccgggc
     1141 cggcatccag ggcccgcagg cggagcaagc ccgccagctt gatcacctcc tggcgggatt
     1201 gcaggtcgtc accgcacacc agaacgtcgg atgcaactgc ctggccctgg gccaggtggg
     1261 tgtaggagag attctggaag gcggccacca cccgtgtctc agacccgagg agggcctgcg
     1321 cctcttccgt ggccgatccc ccgataggtg tccgcaggcg ccggggtctc tccggatcca
     1381 cggggaccgt gacgtccacc aggatcttcc cccggacggc ctcccggatg tccaggagcg
     1441 tcttctcgtg ggcgtcgtag ggaaccgtga ggaccacgat ttccccggta atggcggcct
     1501 gccggttcag catcccggtc gccgccgctc gtcccacctc ccggttgatg gcctcggcgg
     1561 tctgggccgc acgctccagc gcccgcgagc cgatgatcag ctcctgtccc gcgcgagccc
     1621 accccagggc caaaccctgc ccctccttgc ccgtaccccc gacaacggcg attcgcatct
     1681 gctcaccgca gcctaacaag caaatcccaa tgacaaaatc ccaatgacca atggattctg
     1741 agttctcatt ggaatttggt catttgtcat tggtcatttg agatttcccg tccgcgggaa
     1801 agatccggag cagcttgtac gtcgtgctcc gctctgccgg gatccgatcc agctcccaga
     1861 tgcgctcctg catttcttcg ggagacaggt actccccggc agtggccccc gccagtcggg
     1921 agatgttctc ctccatcagc gtaccgctgt agtcgttggc acccgccagc aagcaccgct
     1981 gcgacagctc gcgccccagc ttcacccacg agatctggat gttgtcgatg gcaccctgaa
     2041 gcatgaggcg ggccaaggca tgcaccttga ggtgctcctc cagcgtcggg ccgggccgcg
     2101 tcttcccttc ccggaagaca ttggtgttca ggtggatgaa gcccagtggc acgaactcgg
     2161 tgaaaccgcc cgtctgtctt tggatgtccc gcagcaggca gagctggttg acccagtgcg
     2221 cgggctcttc gacgtggccg tacatcatgg tcgaggtggt ggggatgccc aggcggtggg
     2281 ccgtggtgat gacctccacc cattgcttga ccgtgacctt cagccgggag aggagttggc
     2341 ggaccccgtc gtccaggatc tccgcggccg tccccggcag gctccccagg ccctcatcct
     2401 tcagcatggc caagtaatcg gccaagggca tccccgtctt ctcgacgcca tagaccacct
     2461 ccatgggcga gaaggcgtgg atgtgcatct tgggcgtggc ccgcttcacc gcccgcagga
     2521 tatcccggta gtggtacccg tccaggtcgc gaggcagccc cccctggatg cagacctcgg
     2581 tggcgccacg ctcccatgcc tcggccgcct tgaccgcgat ctgctccagg gtcagatcgt
     2641 aggcgtcgga cgcgttgggg ccggtgctga aggcgcagaa cttacatccg atgatgcaga
     2701 cgttggtgaa gttgatgttc cggttcacca cgtaggtgat gatgtccccc acacggctgc
     2761 ggcggacctc gtcggcgacg gccgtcaggg gaccaaccat gtccggcgga agctcggcca
     2821 ggatcacccc ctcctcgcgg gtcagttccc gcccttccag cgcccgatcc aggatccgag
     2881 cgacggagac gggaacccgc ccgaccgcgc tcccggcagc acacggcaag cccatcatct
     2941 gaatggaaac tcccctctgc taagaaacag cccacaaccg acgtctgaca gttgttgggt
     3001 cgtcaggtga tttcggcagg ctcagtcgag ccgtcggttg cgggtcttgc atttctgtct
     3061 tctggcacca ggccgtcgcc atccacacag gctcggatgc gctctgccag ctcgggcgga
     3121 aggaacgcgg ggcgatccag gacgtactca ggatacaccg gcaagcgctg gcgcaaaagg
     3181 aaaccccggg tctcggtggc agcccgcagg gccgcgaggt gcggccaggg cgcctccggg
     3241 ttgatgaagt cggccgtcac cggtgacacc ccgccccagt cgttgatccc ggcatccagc
     3301 aggcggccgt agtcctgcgt caggttgggc ggcgcctgga ggttcatgta tgcgcccagg
     3361 agcaggcgcg ccaccgctgt ggtccggagc atctcctcca gcgtgggctc cggctgatcg
     3421 tgcatcggga tcctcgcctt ggcccggaag ttctggatga tcacctcctg gacgtgtccg
     3481 tagcgctcgt gcagtacccg gatggccagg agagagtcca cccgttcttc cggcgtctcg
     3541 ccgatcccga tgagcagccc tgtggtgaag gggatccgga gttctcctgc catctccagg
     3601 acgtggaggc gcgcccgcgg atgcttggtc acggctccat ggtgcggccc gccgggcagg
     3661 agcagccggg ggctcgccga ttccagcatg aggcccatgc tggcgttcag gggcttcaag
     3721 gcccggaggt caggctcgcc catcaggcct gcgttcgcat gggggaaaag cggcgagcgc
     3781 gtgaggacca gggcgcaggc ggcgcgcagg taatccagcg tccggcggta tccccggccc
     3841 tgcagaatcg cccgggcctc ggggaagacg gcctcgggcg catcccccag actgaagagc
     3901 gcctcggtgc agccgaggcg cgccccctcc tcgaccac
//
LOCUS       DBNGZC010000808         8138 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1044620, whole genome shotgun sequence.
ACCESSION   DBNGZC010000808 DBNGZC010000000
VERSION     DBNGZC010000808.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 8138)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8138)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8138
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1044620"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1018)
                     /locus_tag="AC16G0_13860"
     CDS             complement(131..1018)
                     /locus_tag="AC16G0_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIR5100733.1"
                     /translation="MPFRADPEKEYYAILGLKEDASPEEIRKAYRKLALHYHPDRNRG
                     DAGAEERFKAISEAYGVLMDPEKRQQYDLSRQAGVGASAGRHPEPGAYSSQEDILRDL
                     LHNRDASAIFEELTREFQRMGFRFDDGFVRHMFFGGRGVVFGGVFFGGPFRGGRRGGG
                     EDIRGYGRRFEPWGPWLQTGRMTETAEEQASPGLLSRLGQAIRGVVGGLGRAAGFALG
                     AGRRGEDLTHELVITPDEARNGARKHVRLARGGDIEEVIVTIPAGVRPGTRLRLRGKG
                     NSSPAGERGDLYLRVQIEL"
     gene            1267..2220
                     /locus_tag="AC16G0_13865"
     CDS             1267..2220
                     /locus_tag="AC16G0_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR5100734.1"
                     /translation="MRREKGVALFVAGLFFCLALAGQAFAGELRIMTGPQGGAWYPLG
                     GAMAEIFKKEIPGTSASVLPGAGIINVQGVETARADIGFGNSVSTVDGLAGREPFKAP
                     VKNVRQLATLYMQYFQLIVLEDSKINDVPGLKGKSIAVQPRGNTGEQMTRELLQVYGL
                     SYKDLGKVSHVSYNDAVALMKDGHTQVYTLISTVPASSVLDLASDRKIRMLSVPEDKY
                     KELLKINSGYARREIPKGTYPGVDYPVEAFGTYTHLMIRGDLPDDLVYGITKALAKHA
                     DSLGAVVKDVQGLTVKEMAFDVGVPYHPGALKYYKEAGVLK"
     gene            2372..4450
                     /locus_tag="AC16G0_13870"
     CDS             2372..4450
                     /locus_tag="AC16G0_13870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HIR5100735.1"
                     /translation="MRGLTGRIGVCLRWLAIAMSLYHLYVGATGPPEALVFRGIHLAF
                     ASTLILLWYPFGARSPQARPAILDWACVLLSLTSLGYLFLRYDYVMTRMSYVTPLERW
                     DLILGIVLTIMVLEATRRTIGPALPITALVFLAYALLGPYLPPLLRHRGFSLELIIDQ
                     LYLTTDGIFGIPLAVSATYVILFVLFGAFLEKSGTGQLFMDFASALAGHTPGGPGKIS
                     CISSGLFGTISGSAVANVMVDGWLTIPLMKRTGFRPHFAAAVEAVASTGGQNMPPVMG
                     AAAFVMAEFLGVPYITIAKHAAIPAILYYLALFFAIHFEAQRTGLRGLPRDELPSVRS
                     VLIDRGHLFMPLLIIVYLMLMGYTAPLAALWGTLAAALSTWMGRLGWLYLVALLIENI
                     LPGNVMEVLGIGWLVLVAIRLMSAEGRAGLKPLLTKLFPDALEDGARNALAVASACAC
                     AGIVIGVIALTGAGLQFTSLVLAAAAESLVPALVLTKIAGIILGMGMPTTAAYIVQAA
                     LLIPALIKLGILPIAAHMFVFYFAIISAITPPVAMAVYAAAGIGKTNLWATGLAAMRL
                     GATGFIVPFMFVYGPSLLFVGTWFDIVTTIVSACIGVVCLSAALHGWLTKPTVWWERV
                     LLGVAAFCLIKPGIYTDILGLVFLAAVLASQWWLDIPRPAIEAKGMAEARGHAVELDA
                     AKIERAKQEG"
     gene            complement(4457..5239)
                     /locus_tag="AC16G0_13875"
     CDS             complement(4457..5239)
                     /locus_tag="AC16G0_13875"
                     /inference="COORDINATES: protein motif:HMM:NF024118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5100736.1"
                     /translation="MPFQFFGTSGAIPSPTRDNTALAFYSGEEAVLVDCSGSPYQKIL
                     KAGLDPMKVSCLIVTHRHVDHVYGIPSLAHNMGLAGRNATLHIHALGETMPSLRGFMQ
                     LFPLEEKMPYRIELHEIPAKEGHEVLRAKGLRIRSTPVKHGAPNIGLRVEFDTGSERG
                     AVVFSSDTSPCPSLLALARGADILIHEATFLHSDAQRAAEDGHTTGYQAGEVARQAGV
                     KRLILCHFSADLQDRLEELRQEALRAYAGPVELPEEFREYRI"
     gene            5959..7209
                     /locus_tag="AC16G0_13880"
     CDS             5959..7209
                     /locus_tag="AC16G0_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5100737.1"
                     /translation="MRRCGWLVLGVFALVLGLSGFVAPVMAADEFKIGVITSLSGDLA
                     TGGSVTKRGYDLWAKAVNAQGGIEIKGKKYTVKLVYGDDQSQPAQAASAAERLAAQEK
                     VDAVLGPYASGTTIAAAPVLEKYKVPMLTGSAESPLIWKQKFRYTFGTIPPVNFTGAT
                     VIKVLAALPQGPKTAVIYGTNDTFSKATAEAFQTTSEQVGIKVLRFNILPPKQDMTPL
                     MSAAKVLNPDLVAFGGHDEELVNLVKVLSQINFTPKALLMHYGVTEPAFIQALGKNAN
                     QVFGASVWTGALQTKGTVGWPTAKAYFEAAVAEYPKDPPDYTQAASSAAGLVFQAALT
                     QLGSAPPLDEGARTRLVDILEKLDLQTFYGRVKFATEGDYYHSNAGLTPLLVQITDGK
                     EAVVAPPESAQAKLVYPMTPWDKR"
     gene            7261..>8138
                     /locus_tag="AC16G0_13885"
     CDS             7261..>8138
                     /locus_tag="AC16G0_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5100738.1"
                     /translation="MLALLPQAIVDGILLGGIYLTVAMGFSLAFGVLHVIDFAVGEWI
                     MLGAFIGLTLTKWFHVDPLLTLPVIFAIFATVGWMLQPLLQRVLSGSRGRPVLMGLVF
                     TFGLALAFRGSALTVFGIFTHSLDTILSQGSLHFSRGSFFLTIPFIRLAGLVYALAVI
                     VTLNYLLKKTDFGLAVRAVAQNKEAAGLMGVDIKRTSGAVDGIYVGISAMTGVFIGAI
                     VSLNASMGPDLALFAFFVVVLAGMGYLSGVPWAAFLLGLIQSFFLIYFNPSYVLLALF
                     TILYLILLISPTGLFR"
BASE COUNT         1367 a         2590 c         2616 g         1565 t
ORIGIN      
        1 actgaggccc ggcgattcgt caacgaactt cggggagagg acattaggtc gactcctcga
       61 ggcgggaaat gggtggacgg gcaggtcgat ccagcagcct gggcccctgg cgatgggata
      121 gggaaaccga tcagagttcg atctggactc gcaggtacag atcgccgcgc tcgcccgccg
      181 ggctgctgtt ccccttgcct cgaaggcgga gcctggtccc cggccgcacc ccggcgggga
      241 tggtgacgat gacctcttcg atgtcgccgc cgcgagccag acggacgtgc ttcctggccc
      301 cgttccgggc ctcgtccggc gtgatgacga gctcgtgggt gagatcctca ccccgccggc
      361 ctgccccaag ggcaaacccg gctgcgcggc cgaggccgcc caccacccct cgaatggcct
      421 ggccgagccg cgagagcaga cccggcgatg cctgttcctc cgctgtctcg gtcatccgac
      481 cggtctgcaa ccaggggccc cacggctcga agcgcctgcc gtatcccctg atgtcctcgc
      541 ccccgccacg acggcctccc ctgaacggcc cgccgaagaa gaccccgccg aataccaccc
      601 cgcggccccc gaagaacata tgccggacga acccgtcatc gaagcgaaat cccatccgct
      661 gaaactcgcg cgtgagctct tcgaaaattg cgctggcgtc ccggttgtga agcaggtccc
      721 tgaggatatc ttcctggctg gagtaggcgc cgggctcggg gtggcgaccg gcggatgcgc
      781 ccacgcccgc ctgccgtgac aggtcgtact gctgccgctt ctcggggtcc atcaggacgc
      841 cgtaggcctc gctgatggcc ttgaagcgct cctcggcccc ggcgtcgccg cggttgcggt
      901 cgggatggta gtgcaaggcc agcttccggt aggccttccg gatctcctcc ggcgaggcgt
      961 cctccttcag gcccaggatg gcgtaatatt ccttctcagg atcggcgcga aagggcatgc
     1021 aagctcctca tggcggaatg gagggcctag tctaccgttc tgcccttgaa gcggcaagga
     1081 gtttcggggc gcggaggttt cgcgtgacca agccgttgac acgcatcctg gtgtcaagta
     1141 agttcaagcc cgcggcaagc ggggaagcag acaggcgccg gacaagcagc ggggggcagg
     1201 gcagggagtg gtgccggaat tgagagccga gggacgagag ggaccgctga aacgggaggg
     1261 gcaatcatga gacgagaaaa gggagtcgcg ctctttgtgg ctggcctgtt tttctgcctg
     1321 gccttggcgg gtcaggcttt cgctggggaa ttgcggatca tgacgggtcc ccagggaggg
     1381 gcctggtacc cgctgggcgg ggcgatggcc gagatattca agaaggagat cccgggcacc
     1441 tctgcgtcag tcctgcccgg cgccgggatc ataaacgtcc aaggggtgga aaccgcccgg
     1501 gccgatatcg gattcgggaa ctcggtctcg acagtggacg gcctggcggg gcgggagccg
     1561 tttaaggcgc cggtaaagaa tgtccgccaa ctcgcgacgc tatacatgca gtatttccag
     1621 ctcatcgtcc tggaggattc gaagatcaat gatgtaccgg gcctcaaggg gaaatccatc
     1681 gccgtccagc ctcgggggaa caccggcgaa cagatgaccc gcgagctgct tcaggtctac
     1741 ggtctctcgt acaaggacct gggtaaggtg agccacgtct cctataacga cgccgtggcc
     1801 ctcatgaagg acgggcacac gcaggtctac acgctcatca gcacggtccc cgcatcctcc
     1861 gtcctggact tggccagcga ccggaagatc cgcatgctct ccgtccccga ggacaagtac
     1921 aaggaactcc tgaagatcaa cagcgggtat gcgcgacggg agatcccgaa gggtacgtat
     1981 ccgggcgtgg attatcctgt cgaggccttc gggacctaca cccacctgat gatccggggg
     2041 gacctgccgg atgatcttgt atacggcatc accaaggccc tggccaagca cgcggacagc
     2101 ctgggggcag tggtgaagga tgtccagggg ctgacggtga aggagatggc cttcgacgtg
     2161 ggggtcccgt atcatcccgg ggcattgaag tattacaagg aagcgggggt gctgaagtag
     2221 ctcctcaaca aacaaatgac caatgaccaa atcccaatgg caaaccccga gagggaggcg
     2281 ggaccaaaag ccagttagtt cccagcgcga agtgctccgc cattggtcat tgggtcattg
     2341 gtcattggga tttccgggga gggggcgtaa cgtgcgaggc ctgaccggtc ggatcggggt
     2401 ctgcctgcgc tggctggcga tcgcgatgtc gctctaccac ctctacgtgg gagccaccgg
     2461 tccgccggag gctctcgttt ttcgtggcat ccacctggcc tttgcctcca ccctgatcct
     2521 gctctggtac cctttcggcg ctcgatcgcc gcaggcccgt ccggcgatcc tggactgggc
     2581 ctgcgtcctg ctcagcctga ccagcctcgg ctacctgttc ctgcgatacg actacgtcat
     2641 gacccggatg tcgtatgtca caccgctcga gcggtgggat ctgattctcg ggatcgtcct
     2701 gacgatcatg gtcttggagg ccacccggcg caccatcggc cctgccctgc ccatcaccgc
     2761 cctcgtcttc ctggcgtatg ccctcctcgg tccatatctc ccgccgctgc tccgacaccg
     2821 gggattctcg ctggaactca tcatcgatca actgtatctg acgacggatg ggatcttcgg
     2881 catcccgctg gccgtctcgg caacctacgt catcctgttc gtgctcttcg gcgccttcct
     2941 ggagaagtcg gggaccgggc agctcttcat ggacttcgcc tcggccctgg cggggcacac
     3001 gccgggcggc ccaggaaaga tctcgtgcat cagcagcggg ctcttcggga cgatttcggg
     3061 cagcgccgtg gccaacgtga tggtggatgg atggctcacg atccccctca tgaagcggac
     3121 cggcttccga cctcatttcg ccgcggcggt ggaggcggtg gcctccaccg gggggcagaa
     3181 catgccgccg gtgatggggg ctgcggcctt cgtcatggcc gagttcctgg gggtgcccta
     3241 catcaccatc gccaagcatg ccgccatccc ggccatcctg tactacctgg cgctcttctt
     3301 cgccatccac ttcgaagcgc agcgcacagg gctgcgcggg ctgccgcggg atgagctgcc
     3361 ttccgttcga agcgtgctga tcgaccgtgg acacctgttt atgccgctgc tcatcatcgt
     3421 atacctgatg ctcatggggt acaccgcccc cctggcggcc ctctggggga ccctggctgc
     3481 ggccctgtcc acgtggatgg gccgactggg ctggctctac ctcgtggccc tgctcatcga
     3541 gaatatcctg ccgggcaacg tgatggaggt ccttgggatc ggctggctgg tcctggtggc
     3601 gatccgcctc atgtcggcgg aaggccgggc gggcctgaag ccgctcctga cgaagctgtt
     3661 cccggatgcc ctcgaggatg gcgcacgcaa cgccctggcc gtggcatccg cctgcgcctg
     3721 cgcgggcatc gtgatcgggg tgatcgcgct gactggggcc ggcctccagt tcactagcct
     3781 ggtcctggcg gccgccgcgg agtcgctcgt tccggccctg gtcctcacca agatcgcggg
     3841 aatcatcctg ggcatgggga tgcccaccac ggccgcctac atcgtgcagg ccgccctcct
     3901 gatcccggcc ctgatcaagc tgggcatcct gcccatcgcc gcccacatgt tcgtcttcta
     3961 cttcgccatc atctcggcca tcacgccgcc ggtggcaatg gcagtgtatg cggcggcagg
     4021 gatcgggaag acgaacctgt gggccacggg tctcgccgcc atgcgcctgg gggccaccgg
     4081 cttcatcgtc cccttcatgt tcgtgtacgg gccgtcgctc ctgttcgtgg ggacgtggtt
     4141 cgacatcgtc accaccatcg tgtccgcgtg catcggggtc gtctgcctct cggcggcgct
     4201 ccacggctgg ctcacgaagc ccacggtgtg gtgggagcgg gtcctgttgg gggtcgccgc
     4261 cttctgcctc atcaagccgg ggatctacac cgacatcctg gggctggtgt tcctggccgc
     4321 agtcctcgcg tcacagtggt ggctcgatat tccgcggccg gcgatcgagg ccaagggaat
     4381 ggcggaggcc cggggccacg ccgtggagct cgacgcagcc aagatcgagc gggccaaaca
     4441 ggaagggtag aagagattag atccggtact cccgaaattc ctcgggcaat tccaccggtc
     4501 ccgcgtacgc ccgcagtgcc tcctggcgca attcctcgag acggtcctgc agatccgcgc
     4561 tgaagtgaca gaggatgagg cgcttcacgc cggcctgccg ggccacctct cccgcctgat
     4621 agcccgtggt gtggccatcc tcggccgccc gctgggcgtc cgagtgcagg aaggttgcct
     4681 cgtggatgag gatgtcggcg ccccgggcca aggcgagaag ggacgggcac ggactggtgt
     4741 cgctactgaa cacgaccgca cctcgttcgg agcctgtatc gaattccacc cggagaccga
     4801 tgtttggcgc cccgtgcttc acgggcgtgg accggatgcg gagccccttg gcccggagaa
     4861 cctcgtgtcc ctccttggcc gggatctcgt gcagctcgat ccgatagggc atcttctctt
     4921 cgagggggaa aagctgcatg aacccgcgca gactgggcat ggtttcaccc agggcgtgga
     4981 tgtgaagggt ggcgtttcgt ccggccagtc ccatgttgtg cgccagggac gggatgccat
     5041 acacatggtc tacgtgccgg tgcgtcacga tcaaacacga gaccttcatc gggtcgagcc
     5101 ccgccttcag gatcttctgg tacgggcttc ccgagcagtc cacgaggacg gcttcctctc
     5161 ccgagtaaaa ggccaaggcg gtgttgtccc gggtcggcga ggggatggcg ccggacgttc
     5221 cgaagaactg gaagggcatg gcaacctcct gaaggcgtga ggatgtccgg cacgctgccg
     5281 gggaatgtcg cctcggcgaa cgaggcgccg aagccaagat acactttccc gtggatttca
     5341 ctctagcaga gatccttttg gctcgtctat cggttttccc tccacggtcc tggcgacggg
     5401 cgtggcggtc ccttggcggg cagtgtcgca tatcggccat gaagtgacac ggcagcaggc
     5461 tgcccaaaaa aagaatatgt ggcgtctcaa attctaatag tgtatctgtt agccgtattg
     5521 ctgcagcggt gaggctgccg ggatccctcg cggatgaggc gttgcctggc tctcccgcct
     5581 tcggggcaag aaaagaatcg cacgccatct gccacatcgg ggagcggggg atgaagactt
     5641 ctcgggtcgg gtgacacctc cgactgatca ttcagatcgc aaacgatgag gtgagttttt
     5701 ccggggctcc cttgccgtaa cgggcgaggt tggtgcctct ggtctcgtcg tcgagatggc
     5761 cggctcgtat agcgggcagt ctcatccaaa ggtggctacg ccggggcgtt acgacaagcg
     5821 ctaatcggcc ggaaggcccg gtgaatgaga cggctggcgc agaactttct agtccgctca
     5881 ttctccccgt ttccatctcc ggctgatttg ttgtctgatt acctgaatcg cttcacattt
     5941 gaaaaaggga ggggagccat gcgcagatgc gggtggttgg tgcttggcgt gttcgccctg
     6001 gttctcggcc tgagcgggtt cgtcgctccc gtcatggccg ccgatgagtt caagatcggc
     6061 gtcatcacat ctctctccgg cgacctcgcc accggcggca gcgtcaccaa gcgcgggtac
     6121 gatctgtggg cgaaggctgt caacgcccag ggcggcatcg agatcaaggg gaagaagtat
     6181 acggtcaagc tcgtctacgg agacgatcaa tcccaacccg cccaagccgc ctcggcggcc
     6241 gagcgcttgg ccgctcagga aaaggttgat gccgtactcg ggccgtatgc ctcggggacg
     6301 acgatagccg cggcgcccgt cctggagaag tacaaggtcc ccatgctcac cggctcggcc
     6361 gagtcgccgc tcatctggaa gcagaagttc cgctacacct tcggcaccat cccgcccgtg
     6421 aacttcacgg gggcgacggt catcaaggtc ctggccgctt tgccccaggg gcccaagacg
     6481 gcggtgatct acgggacgaa cgataccttc tccaaggcga ccgccgaggc cttccagacc
     6541 acctccgagc aggtggggat caaggtgctt cggttcaaca tcctgccgcc caagcaggat
     6601 atgaccccgc tcatgtcggc cgcgaaggtg ctcaaccccg acctagtcgc cttcggcggc
     6661 cacgatgaag aactggtcaa cctggtcaag gtgctcagcc agatcaactt cacgcccaag
     6721 gcgctcctga tgcactatgg cgtcaccgag ccggccttca tacaggccct ggggaagaat
     6781 gccaaccagg tgttcggcgc ttcggtctgg accggtgcct tgcaaaccaa ggggaccgtg
     6841 gggtggccga cggccaaagc ctacttcgaa gctgccgtgg cggagtatcc caaggacccg
     6901 ccggactata cgcaggcggc gagctcggcg gccggtttgg tcttccaggc ggccctgacc
     6961 caactggggt cggccccgcc gctggatgag ggggcccgga caaggctggt tgacatcctg
     7021 gagaagcttg acctgcagac attctatgga cgggtgaagt tcgccactga gggagactac
     7081 tatcactcca atgccggcct gacgccattg ctggtccaga tcaccgatgg caaagaggcg
     7141 gtggtggcac cgcccgagag cgcccaggcc aagctggtct accccatgac accctgggac
     7201 aagcgataat cgcaggccga cgggtcgggg cggagcgatt ccgccccgac ccttgagacc
     7261 atgctcgccc tccttccgca agccatcgtt gacggcatcc tgctcggcgg tatctacctg
     7321 accgtcgcca tgggattctc tttggccttc ggcgtgctgc acgtcatcga ttttgccgtg
     7381 ggcgaatgga tcatgctggg cgccttcatt ggcttgaccc tcaccaagtg gttccacgtg
     7441 gatcccctct tgaccctgcc ggtgattttc gccatcttcg ccacagtcgg ctggatgctg
     7501 cagcccttgc tgcagcgggt cctgagcggc tcgcggggcc ggccggtgct catgggtttg
     7561 gttttcacct ttggcttggc tctggccttc cgggggtcgg ccttgaccgt cttcggcatc
     7621 ttcacccact cgctggatac gatcctgtcg caaggcagtc tgcacttcag tcggggctcc
     7681 ttctttctga cgatcccgtt catccggctg gccggactgg tgtacgccct agcggtgatc
     7741 gtgaccttga attaccttct caagaagacc gacttcggcc tcgccgtgcg cgccgtcgcc
     7801 cagaacaagg aggcggccgg tttgatgggg gtggacatca agcgaaccag cggggccgta
     7861 gacgggatct acgtcggcat cagcgcgatg acaggcgtct tcatcggggc catcgtgtct
     7921 ctgaacgcca gcatggggcc ggacctggca cttttcgcct ttttcgtggt cgtcctggcc
     7981 ggcatgggct atctgtccgg ggtgccctgg gcggcgttcc tgctgggact gatccaatcc
     8041 tttttcttga tctatttcaa ccccagctac gtgctcttgg ccctgttcac catcctctac
     8101 ctcatcctgt tgatctcgcc caccgggttg ttccgcaa
//
LOCUS       DBNGZC010000809         2233 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2512303, whole genome shotgun sequence.
ACCESSION   DBNGZC010000809 DBNGZC010000000
VERSION     DBNGZC010000809.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2512303"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="AC16G0_13890"
     CDS             complement(<1..800)
                     /locus_tag="AC16G0_13890"
                     /inference="COORDINATES: protein motif:HMM:NF012703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HIR5100739.1"
                     /translation="MSLPFSGIATFAKVPLYSPDETPAPRAAILGIPTDEGTTQHPGA
                     RYGPRAIREASTQFPYYKRGRGYYDPERDRPMLADLELRDAGDVDIIPTLLQQNADRI
                     AGAVRGLRERGIFPVCLGGDHSVTPAILPAFADTPIHLVQIDAHMDFVDRLAGAPATH
                     ASPMRRARELPHVKSLTQIGIRSIVSSAEDYAAARAAGNRIVTGRQVLEAPGHDWWAD
                     LAGDAYLTLDIDAFDPSVAPGTGFAEPGGLVFRDLAGLLPFLTARLRLR"
     gene            complement(797..1324)
                     /locus_tag="AC16G0_13895"
     CDS             complement(797..1324)
                     /locus_tag="AC16G0_13895"
                     /inference="COORDINATES: protein motif:HMM:NF010248.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTPase"
                     /protein_id="HIR5100740.1"
                     /translation="MGTALLISGAPGSGKTTLIRGMVAELPCRAGGFFTEEIREGEKR
                     VGFRVKALDGRTGILAHVKSVQGPRVGRYRVDVASFEAVGVDALEAATQAADLIVVDE
                     IGKMELCSPRFISALEAALQSRKPILGTILQASHPWTDTIKRRPAVDLYRLTEGNREH
                     LKDALLARLLTEIRE"
     gene            complement(1448..>2233)
                     /locus_tag="AC16G0_13900"
     CDS             complement(1448..>2233)
                     /locus_tag="AC16G0_13900"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5100741.1"
                     /translation="GALQRRDLEYALYQFRQAVRLNPSMVEAHHNLGIALLELRQPGP
                     AAEAFSRALELAPDHLEARLGLGLAYRASGKPIQAIAELTQVLPKRPDDPQLRMALGD
                     LYRTTKQPVLAAAQFRQAARLTPNDPAVHFQLGLAYADAGETDQAIEAFRKAVSLRPE
                     DADAYYNLGLVVGDKIRALLDERVAAYQKAVELRPNLADAHYNLGVALIQKAQIQTGQ
                     TEEKHALLQLALEQFRLFQQFAPSDPKAASAAHNIQVLEPQVK"
BASE COUNT          345 a          731 c          786 g          371 t
ORIGIN      
        1 cggaggcgga ggcgggccgt caggaatggc aggagacctg ccaggtcacg gaatacgagg
       61 ccccccggct ctgcgaaccc cgtgccaggc gccaccgaag gatcaaaggc gtcgatgtcc
      121 agggtcaggt aggcgtcgcc cgcgaggtcc gcccaccagt cgtgccccgg cgcttccagg
      181 acttgcctac cggtcacgat gcggtttccc gccgcccggg ccgccgcgta gtcctcggcg
      241 ctggacacga tgctgcggat gccgatctgg gtgaggctct tcacgtgggg gagctcgcgc
      301 gcacgccgca ttgggctggc gtgggtcgcc ggcgctccgg cgagtcggtc cacgaagtcc
      361 atgtgggcgt cgatctgaac cagatggatg ggcgtgtcgg cgaaagccgg caggatggcg
      421 ggggtgacgc tgtgatctcc gcccaggcag acggggaaga tcccacgctc gcggagcccc
      481 ctcaccgctc ctgcgatgcg atcggcattc tgctgcagga gggtggggat gatgtccaca
      541 tcgccggcat cgcggagctc caggtcggcc agcatcggtc ggtcccgttc cgggtcgtag
      601 tatccgcgcc cccgcttgta atagggaaat tgggtggaag cctcgcggat cgcccggggg
      661 ccgtagcgcg cgcccgggtg ctgcgtggtt ccctcgtcgg tcggaatccc caagatcgcc
      721 gcgcgggggg cgggtgtctc gtccggacta tacagaggca ccttggcgaa ggtggcgata
      781 ccggaaaagg ggaggctcac tcccggatct ccgtcaagag gcgggccaga agggcgtcct
      841 tgagatgctc gcggttcccc tccgtgagac ggtacagatc caccgcaggg cgccgcttga
      901 ttgtatcggt ccacgggtga gacgcctgga ggatggtgcc caggatcggc ttccgcgatt
      961 gcagcgccgc ctccagggcc gagatgaagc gaggggagca gagctccatc ttgccgatct
     1021 catccaccac gatcagatcg gctgcctgcg tggccgcctc cagagcatcc acgcccacgg
     1081 cctcgaagct cgccacatcc actcgatagc ggcccaccct cggcccctgt acggacttca
     1141 cgtgggccag gatgccggtc cgcccgtcca gggccttcac ccggaagccg accctctttt
     1201 cgccctcccg gatctcctcc gtgaagaagc ccccggcgcg gcatggaagt tcggcaacca
     1261 tgccgcggat gagggtggtc ttgccggatc cgggtgcacc ggagatcaag agcgcggtgc
     1321 ccatggccgt tcctcccggc ccgcagtgtc cgggcctttc acctggggga ccggatcgct
     1381 ctgtgctcgg ccgcagcggg tcccagacat cacccgtttt gcccgggggc taatcccccc
     1441 agccgcctca cttcacctga ggctcgagga cctggatatt gtgggcggcc gacgcggcct
     1501 tgggatcgga cggtgcgaac tgctggaaca gtcggaactg ctccagggcg agctggagca
     1561 gcgcgtgttt ctcctccgtc tggccggtct ggatctgcgc cttctggatc aaggccacac
     1621 cgagattgta gtgagcgtcc gctaggttgg gacggagctc gacggccttc tgataggcgg
     1681 ccaccctctc gtccaggagc gcccggatct tgtcccccac gaccaggccc aggttatagt
     1741 aggcgtcggc atcctcaggg cggagggata cggccttccg gaacgcctcg atggcctggt
     1801 cggtctcgcc agcgtcggcg taggccagcc cgagctggaa gtgcacggcg gggtcgttgg
     1861 gggtgagacg agcggcctgg cggaactgcg cggcggcgag gacgggctgt ttggtcgtcc
     1921 ggtagaggtc gcccagggcc atgcgcaact ggggatcgtc cggccgcttc ggcaggactt
     1981 gagtcaactc ggcgatggcc tgaatgggtt tgccgctggc acgataggcc agccccaggc
     2041 cgaggcgcgc ctccagatga tccggggcga gttccaacgc ccggctgaaa gcctccgcag
     2101 ccgggccggg ctgtcggagc tccaggaggg cgatgcccag attgtggtgg gcctcgacca
     2161 tcgagggatt cagccgcact gcctggcgga actggtacag ggcatactcc agatcacgcc
     2221 gctgcaatgc ccc
//
LOCUS       DBNGZC010000810         2190 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1731450, whole genome shotgun sequence.
ACCESSION   DBNGZC010000810 DBNGZC010000000
VERSION     DBNGZC010000810.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1731450"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..>2190)
                     /locus_tag="AC16G0_13905"
     CDS             complement(168..>2190)
                     /locus_tag="AC16G0_13905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIR5100742.1"
                     /translation="YPRYLGLRLSEAYRRLPGWVHPVFAAVAPRLIREAETSRNRGDW
                     IRRFIAGTVQPLPGRYIGWTRFFSEEDLARLAMPGLREQWKADVEAIPRAVFAASHPG
                     DPVDGAFRIDLSTYLPDDLLVMADRMSMAHSLELRAPFCDHRVIEESLGIPSRMKIPG
                     FRLKGLLKMAFADVLPQQVLSHQKQGFMIPLARWLRTDLREVMEDLLSRERVRKRGLF
                     VPEAVEALKQEHLRGIRTHSDRLWTLMIAELWMRQYLDSDESRQASDLRAGHAVHTPI
                     ASAVSPLPAKEMLEPFGCEQVSPPAEQTQAIPRSQPSLAGPEQEPGGEPQRMLVLNVA
                     GLGDFVMGTPALRALRQRFPLAQIWILIIPEVRGLAERCPYVDVVRTLDLRNSRSALA
                     WALGPKRRELRQLIRELRDMRFDTAVNLYRVATRGGGVRMTAFLRAIGASRTVGRYSG
                     GLGASFNLTSMTEGHETDAQLGVARLLGAPPTSQYPELWVTADDRAICHSLLRRHGVS
                     VADPVVCLHPGSARPEACWPAERFAVVGRRLAEVGARVVLFGASGHRALCARIANAIP
                     RAVSLAGETSLPVLAALLQRAVLLVTNDSGPMHMAAALGVPLVVPFGPADPAHTGPRG
                     PAEHVVFPASAGSNCSPWWKDVPAEVVADAAVRLFAESSARSPNAGDDA"
BASE COUNT          398 a          693 c          742 g          357 t
ORIGIN      
        1 cacgctggcg ttggacaggc tttccgccaa tcccggcggc gagtctgacg tctgtccgga
       61 gaccaggctg ctcctgaact ccgacggagg cacccgcgta gcggtgatca gcgtgttctc
      121 cgtggaccct cccacgtcca gccgtgtaat gatgtggagg atgcgaatca tgcgtcgtca
      181 cccgcgttgg gcgagcgggc cgaggactcg gcgaagaggc gaaccgcggc atcggcgacc
      241 acctcggcag gcacgtcctt ccaccagggc gagcagttcg agccagcgct ggcggggaaa
      301 acgacatgtt ctgccggtcc cctggggccg gtgtgggctg ggtcggcggg gccaaagggc
      361 accaccaggg gaactccgag ggctgcggcc atgtgcatgg gaccgctgtc gttcgtgacg
      421 aggagaacgg cccgctgcag gagggcagcc aggacgggga gggatgtctc accggccagg
      481 gagaccgctc tggggatggc attggcaatc cgtgcacaca gggcgcggtg gccggaagcc
      541 ccgaacagca ccacccgggc tcccacctcg gccaatcgcc tgccgaccac ggcgaagcgt
      601 tccgcaggcc aacaggcctc gggccgggca gagcccggat gcagacacac aacagggtcg
      661 gccacagaga caccgtgtct gcgcaagagg gagtgacaga tcgcccgatc atctgccgtg
      721 acccacaatt cggggtattg gctcgtgggc ggtgcaccga ggaggcgcgc gaccccaagc
      781 tgggcgtcgg tctcgtggcc ctctgtcatc gaggtcaggt tgaagctggc gccgagcccc
      841 ccgctgtatc gtcccacggt acggctggct ccgatcgccc ggaggaatgc cgtcatgcgc
      901 acacctcctc ctcgggtggc caccctataa aggttgaccg cggtgtcgaa gcgcatatcc
      961 cgaagctccc ggatcaattg ccgcagttcc cgccgcttcg gtccgagagc ccacgccagc
     1021 gccgagcgcg agttccgcag atccagggtt cggacgacat ccacataggg acagcgctcc
     1081 gctaggcctc tgacctctgg aatgatcagg atccagatct gtgccagggg aaaacgctgg
     1141 cggagagccc gcagtgccgg cgttcccatc acgaagtccc ccagacccgc cacgttcagg
     1201 acgagcatcc gttgcggctc gccccctggt tcttgctcgg ggcccgccag ggatggctgc
     1261 gacctcggaa tcgcctgggt ctgttcggcc ggcggtgaca cctgttcgca gccaaacggt
     1321 tccagcatct ctttagcggg aagtggtgat accgccgagg cgataggggt gtgaacggcg
     1381 tgtcccgccc ggagatcact agcttggcgg ctctcatcgg agtccaggta ctggcgcatc
     1441 cagagttcgg cgatcatgag ggtccacagg cgatcgctgt gggtccgaat gcctcgcagg
     1501 tgttcctgct tcagggcctc taccgcttct ggaacaaata gtcctcgctt ccggacgcgt
     1561 tcgcgggaga ggagatcttc catgacctct cgcaagtccg tccgcagcca tcgggccagg
     1621 ggaatcatga agccctgctt ttggtgggac aggacctgct gcggcaggac gtcggcaaaa
     1681 gccatcttga gaagcccctt gaggcggaag cccggaatct tcatgcgcga cggaatgccc
     1741 agggattcct cgatgactcg atggtcgcag aagggtgccc ggagctccag ggagtgggcc
     1801 atactcatcc ggtctgccat gaccaggaga tcgtcgggaa ggtaagtgct gaggtcaatg
     1861 cgaaacgcgc cgtccactgg atcgccaggg tggctggcgg cgaagacggc tcgggggatc
     1921 gcctcgacat cggccttcca ctgctctcgc aggccaggca tggcgagacg tgcgagatcc
     1981 tcttcggaaa agaagcgagt ccagccgatg taccgaccgg gcaggggctg aactgttccg
     2041 gcgatgaatc gacggatcca atcgccccga tttcggctcg tctccgcctc gcgtattagc
     2101 cgcggggcta ccgccgcgaa gactgggtga acccagccgg ggagtcggcg gtaggcctcc
     2161 gacaaacgga ggcccagata gcgggggtac
//
LOCUS       DBNGZC010000811         4108 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2322875, whole genome shotgun sequence.
ACCESSION   DBNGZC010000811 DBNGZC010000000
VERSION     DBNGZC010000811.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2322875"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="AC16G0_13910"
     CDS             <1..506
                     /locus_tag="AC16G0_13910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013426.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE family
                     protein"
                     /protein_id="HIR5100743.1"
                     /translation="QMSNALLRTPQDPLGVPAQKAVVDRIIEGARGTPGAAMVVLNDL
                     QSQIGFVSLPMQEYVARRLRVPRSQVHGVVTFYSFFTTKPRGTHTVKLCLGTACYVGG
                     VTKLIDKAQKLLGVRVGETTEDWKITLESCRCVGACSQAPIVTVDGDVYGRVRPEQLP
                     EILTRYA"
     gene            518..1069
                     /locus_tag="AC16G0_13915"
     CDS             518..1069
                     /locus_tag="AC16G0_13915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5100744.1"
                     /translation="MKEIALHILDIVENSLDAGATAIDVLVDDDAACNRLRVSVRDDG
                     RGMDARTLAAIDDPFVTSRTTRVAGLGVPLFKEAAEACNGRLEVRSTPGQGTTLEAEF
                     QRDHIDRMPLGDLPGTLLGLVVGRPEIHWHFRYRAGGETFSFDSDPVRQALGDVPLSQ
                     PAALRCIRQTLEEGFRSAQGAAA"
     gene            1120..1509
                     /locus_tag="AC16G0_13920"
     CDS             1120..1509
                     /locus_tag="AC16G0_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HIR5100745.1"
                     /translation="MGTIKSIQDLQRIRAEALEKRRAKSTEGRAQVTVFMGTCGIAAG
                     ARDAMRAILEVIETEKLEGIVVRQTGCIGLCANEPLVEVAVGDGPAVRYGRVSRERAL
                     RIMHEHVAAGTPVAEYAIPVQGQGSDR"
     gene            1506..3341
                     /locus_tag="AC16G0_13925"
     CDS             1506..3341
                     /locus_tag="AC16G0_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013432580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HIR5100746.1"
                     /translation="MIFYRSHILVCMDPECLEKGAAALRDRLCRELAARGLDQEIRVL
                     ETPRLGDCAHGPEIMVYPDEVHYSGLTLEDVPGLVQEHFLKGRVVPRLLSSMKPPSDE
                     ELGPPRPKEVRVVLRNCGVIDPESIEDYIAEDGYQALAKVLAEMAPAQVIEAVLASGL
                     RGRGCAGFPTGKKWQFARAAQGSIKYVICNADEGDPGAFMNRRVLEGDPHAVLEGMII
                     AAYAIGASRGYIYCRAEYPIAVRTLNIAIRQAREYGFLGEGILGSEFAFDLEVRMGAG
                     AFVCGEETALMASIEGQRGHPRTRPPFPAVSGLWGKPTNINNVETYANVPRIILRGPE
                     WFARMGTERSKGTKTFALAGDVSHTGMIEVPLGISLREIVYEVGGGIKGGKQFKAIQT
                     GGPMGGCLPASFLDLPVDYESLTAAGSIMGSGGMIVMDEDTCMVDIARFFMEFTQDES
                     CGKCVPCRVGTKHILETLERICGGEGKPADLGELEHLCEQIRRTSLCGLGQGAPNPVV
                     STLTHFRQEYEAHILEKRCPSLVCQALIHFWIDPEKCNGCHLCAKHCPTEAIIGKPKT
                     VHAILQEKCIKCGNCVEVCPDRFGAATKVAGRAPAGERDTVSAGR"
     gene            3374..>4108
                     /locus_tag="AC16G0_13930"
     CDS             3374..>4108
                     /locus_tag="AC16G0_13930"
                     /inference="COORDINATES: protein motif:HMM:NF024900.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR5100747.1"
                     /translation="MNDVHLSINGQGVIAESGLTILAAARAAGIYIPSLCHHPAVSSH
                     GACRICLVEVKGMRGLQTACTCPVTEGMEVATETEAIAAGRRFVLELLFSERNHYCMF
                     CQVSGDCELQALAYRYGLDHWRYPRPFKPLGLDASHPYILMEPNRCILCARCVRACAE
                     IAANHTLGLCERGSDSMLMADLGVPLGDSTCVGCGTCLQVCPTGALLDARSAYGGHEE
                     ELTHTRTTCMQCSVGCALDVQSRGGRL"
BASE COUNT          685 a         1324 c         1477 g          622 t
ORIGIN      
        1 cacagatgag caacgcgctc cttcggaccc cccaggatcc gctgggggtc cccgcccaga
       61 aggcggtcgt ggatcgcatc atcgaggggg ccagggggac ccctggcgcc gccatggtcg
      121 tgctcaacga cctgcagagc cagatcggat tcgtctccct ccccatgcag gaatacgtgg
      181 cccgccggct ccgggtaccc aggagccagg ttcacggcgt ggtcaccttc tactctttct
      241 tcaccacgaa accccggggg acgcataccg tcaagctgtg cctgggaacg gcgtgctacg
      301 tcggcggagt cacgaagctg atcgacaagg ctcagaagct tctgggggtt cgagtgggag
      361 agacgacgga ggactggaag atcacgctgg agagctgccg gtgcgtgggc gcgtgcagcc
      421 aggcccccat cgtgacggtc gacggcgacg tgtacgggcg ggtccggccg gagcagttgc
      481 cggagatcct cacccggtac gcgtgaggcg ggccagcgtg aaggagattg cgctgcacat
      541 cctggacatc gtcgagaaca gtctcgacgc aggcgccacg gcgatcgacg tcctggtgga
      601 tgacgacgct gcctgcaatc gcctgcgggt gtccgttcga gacgacggcc gggggatgga
      661 tgcgcgaacg ctggccgcca ttgacgaccc gttcgtgacg agccgcacga cccgggtggc
      721 gggactgggg gtaccgctct tcaaggaggc ggccgaggcc tgcaacggcc ggctggaggt
      781 ccgatcgacg ccggggcaag ggacgaccct ggaggcggag ttccagcggg accacatcga
      841 ccggatgccg ctcggcgacc tgccaggcac cctgctcggc ctggtggtcg ggaggcccga
      901 gatccactgg cacttccgtt accgggcggg tggcgagacg ttcagcttcg acagcgatcc
      961 cgtccgccag gcgctggggg atgtgccgct gagccagccg gccgcgctca ggtgcatccg
     1021 gcaaacgctg gaagagggat tccgcagcgc tcagggcgcc gcggcgtgag tgcgggggtc
     1081 tcggattctg tggagatgcc ggcaggcggg aggcgagacg tggggacgat caaatccatc
     1141 caggacctgc agcgcatccg ggccgaagcc ctggagaagc ggcgggccaa gagcaccgag
     1201 ggccgcgccc aggtcacggt cttcatgggg acctgcggca tcgccgccgg cgcgcgagat
     1261 gccatgcgcg ccatcctcga ggtgatcgag acggaaaaac tcgaggggat cgtggtgcgg
     1321 cagacgggct gcatcggtct gtgcgccaac gagccgctgg tggaagtggc ggtcggggat
     1381 gggccggcgg tccggtacgg ccgcgtgtcc cgggagcgcg ccctgcgcat catgcacgag
     1441 cacgtggcgg ctgggacccc cgtggccgag tacgccattc cggttcaggg acagggtagc
     1501 gaccgatgat attctatcgc tcgcacatcc tcgtctgcat ggatcccgag tgcctggaaa
     1561 agggggcggc agctcttcgg gaccgcctct gccgggagct ggccgcccgc gggctggacc
     1621 aggagatccg cgtcctggag accccccgcc tgggggattg cgcccacggc ccggagatca
     1681 tggtctatcc ggacgaggtc cactactccg ggctcaccct ggaggatgtg ccgggtctgg
     1741 tgcaggagca cttcctgaag gggcgggtcg tcccccgcct gctctcttcc atgaagcctc
     1801 cctcggacga agagctgggc ccgcctcgcc ccaaggaagt ccgggttgtc ctccggaact
     1861 gcggcgtcat cgacccagag agcatcgagg actacatcgc ggaggatggc taccaggccc
     1921 tggcgaaggt gctggctgag atggcgcccg cccaggtgat cgaagcggtc ctggcctccg
     1981 gtctccgagg gcggggctgc gccggcttcc cgaccgggaa gaagtggcaa ttcgcccgcg
     2041 cggcccaggg ctcgatcaag tacgtgatct gcaatgccga cgagggcgac ccgggggcgt
     2101 tcatgaatcg ccgggtcctg gagggggacc cgcacgccgt cctggagggc atgatcatcg
     2161 ccgcctacgc catcggcgcc agccgcggct atatctactg ccgggccgaa taccccatcg
     2221 ccgtgcggac gctgaacatc gccatccggc aggccaggga gtacggcttt ctgggcgagg
     2281 gcatcctggg cagcgagttc gccttcgacc tggaggtacg gatgggggcc ggggccttcg
     2341 tctgcggcga ggagacggcc ctcatggcct ccatcgaggg gcagcgcggc catcctcgca
     2401 cgcgcccacc cttccccgcc gtcagcggcc tgtggggcaa gccgaccaac atcaacaacg
     2461 tcgagaccta tgccaacgtg ccccggatca tcttgcgggg gccagagtgg ttcgcccgca
     2521 tgggcacgga gcggagcaag ggcaccaaga cattcgcgct ggcaggcgac gtgagccaca
     2581 ccggcatgat cgaggtgccc ctgggcatca gcctgcgcga gatcgtctac gaggtggggg
     2641 gcggtatcaa gggtgggaaa cagttcaagg ccatccagac cggcgggccc atgggcgggt
     2701 gtctgccggc gagcttcctg gacctgccgg tggactacga gtccctgacg gccgcgggct
     2761 ccatcatggg gtcgggcggg atgatcgtca tggacgagga cacctgcatg gtggatatcg
     2821 cccgcttctt catggagttc acccaggacg agagttgtgg caagtgcgtg ccctgccgcg
     2881 tgggcaccaa gcacatcctg gaaaccctgg agcgcatctg cggcggcgag gggaagccgg
     2941 cggacctcgg ggagctggag catctctgcg agcagatccg gcggaccagc ctgtgcgggc
     3001 tgggccaggg cgcccccaat cccgtggtga gcaccctcac gcacttccgc caggagtacg
     3061 aagcccacat cctggagaag cggtgcccga gcctggtctg ccaggcgctg atccacttct
     3121 ggatcgaccc ggagaagtgc aacggttgcc acctgtgcgc gaagcactgc ccgactgagg
     3181 cgatcatcgg caagcccaag acggtccacg ccatcctgca ggagaagtgc atcaagtgcg
     3241 gcaactgcgt ggaagtgtgc cccgatcgat tcggcgcggc caccaaggtg gccgggcgcg
     3301 caccggcagg cgagcgggac acagtttcgg cgggccgcta gcggttctcg taggtgcggg
     3361 gacagaggat tcgatgaacg acgtgcatct cagcatcaac ggccagggcg ttattgccga
     3421 gagcggtctg acgatcctgg cagcggcgcg tgcggccggg atctatatcc ccagcctgtg
     3481 ccatcacccg gcggtctcca gccacggcgc ctgccgcatc tgcctggtgg aggtgaaggg
     3541 catgcgcggg ctgcagacgg cctgcacctg cccagtgacg gaggggatgg aagtcgcgac
     3601 ggagacggag gcgatcgcgg caggccggcg cttcgtcctg gaactcctct tctccgagcg
     3661 gaaccactac tgcatgttct gccaggtgag cggggactgc gagctgcagg cgctggcgta
     3721 ccgttacggc ctggatcact ggcgctaccc gcgccccttc aagcccctcg ggctggacgc
     3781 ctcccaccca tacatcctca tggagccgaa tcgctgcatc ctctgcgcgc gctgcgtccg
     3841 cgcctgtgcg gagatcgcgg cgaatcatac cctggggctc tgcgagcgcg ggtccgacag
     3901 catgctcatg gccgatctgg gggtcccgct gggcgactcc acctgtgtgg gctgcgggac
     3961 gtgtctccag gtctgcccca cgggggcgct gctcgacgcc aggagcgcat acggcggcca
     4021 cgaggaagag ctcacgcaca cccggaccac ctgcatgcag tgcagcgtgg gctgcgcgct
     4081 ggatgtccaa tcgcgcggcg gccgactg
//
LOCUS       DBNGZC010000812         1790 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1341161, whole genome shotgun sequence.
ACCESSION   DBNGZC010000812 DBNGZC010000000
VERSION     DBNGZC010000812.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1341161"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1574)
                     /locus_tag="AC16G0_13935"
     CDS             complement(156..1574)
                     /locus_tag="AC16G0_13935"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HIR5100748.1"
                     /translation="MTQATPTPPRQQFDSLREQLGKQHGLPFLKLLSRPLVEQACRLC
                     NHVWRERIYTPWITLAIFLSQILSDDHSCDDAVEHFQKVRYDQGLPAVAPETSSYCEA
                     RQRLPEALTWELVRRTGQAIHRQAQAAWLFHGRSVKIIDGSTVTMPDTPGNQAAYPQL
                     DSQAPGLGFPIARLLVVFSLAVGTVLEAALGPYQGKQTGELALLRQVFDQFQPGDIAL
                     ADRFFGSYWMIAALQARGVAVVMRLHQARRADFRRGRRLGREDHLVTWTKPPHVPDWM
                     SRAEYDAMPAQLTVRELRVRVRDRTKRVRDLVIITTLTDRATYPAEELGSLYRQRWHA
                     ELDLRSLKTEMRMEMLRTKTPEMVRKEVAIHLLAYNLIRGIMAEAARVEEVSPRRLSF
                     SGALHTVRSFEEGHLYDPARIAADLPRLLELIGQKRVGDRPDRYEPRAVKRRPKPYPR
                     LKLPRRVAKRLIKRGIRLYEKA"
BASE COUNT          298 a          586 c          594 g          312 t
ORIGIN      
        1 gcttccgaac ggtcggaatc gccgaaaagg atcgaataga gcgggattct ctactttaac
       61 tttaccatca aattcgtgtc aacccgaatg gcaccaggat aacgcaagct cttttctctg
      121 acagaactgc gttgacgatt cgatcgtctc cgatcctatg ccttctcgta gagccttatg
      181 cctcgtttga tcaaccgttt ggctaccctc cgcggcagct tcagccgcgg atacggcttc
      241 ggccgtcgct tcaccgcgcg cggctcgtaa cggtccgggc gatcgcctac gcgcttctga
      301 ccgatcaact ccaacagccg cggcaggtcc gccgcgatcc gcgccgggtc gtacaggtgg
      361 ccctcctcga acgaccggac ggtatgcaac gcgcccgaga agctgagcct ccgcggactc
      421 acctcctcga cccgcgccgc ctccgccatg atcccgcgga tcaggttgta cgccagcagg
      481 tggatcgcca cctccttgcg caccatctcc ggggtcttgg tccgcaacat ctccatccgc
      541 atctcggtct tcagcgagcg cagatccaat tccgcgtgcc accgctgccg gtacaggctc
      601 cccagctcct cggcgggata ggtcgctcga tccgtgagcg tcgtgatgat gaccagatcg
      661 cgcacccgct tggtccgatc ccggacccgg acgcggagct cgcggaccgt gagctgagcg
      721 ggcatcgcgt catactcggc acggctcatc cagtcgggga cgtggggagg cttcgtccaa
      781 gtgacgaggt ggtcctctcg gcccaggcgg cgcccgcgcc ggaagtcggc ccggcgggcc
      841 tggtggagcc gcatcacgac tgcaacgccg cgggcctgca aggccgcgat catccagtac
      901 gagccgaaga accggtcagc cagggcgatg tcgccgggct ggaactgatc gaaaacctgg
      961 cggaggaggg ccagttcgcc ggtctgcttg ccctggtacg gccccagcgc ggcctcgagc
     1021 accgtgccca ccgccaggct gaagaccacg aggagccgcg cgatcgggaa cccgaggccc
     1081 ggcgcctggc tgtccagctg gggataggcc gcctggttcc ccggggtatc gggcatggtc
     1141 acggtggagc cgtcgatgat cttgaccgag cgcccgtgga acaaccaggc ggcctgggcc
     1201 tggcgatgga tcgcttgccc ggtccggcgg acgagctccc aggtcagcgc ctcgggcagg
     1261 cgttggcggg cctcgcagta gctgctcgtc tcgggggcca cggcggggag accctgatcg
     1321 tagcggacct tctggaagtg ctcgacggcg tcgtcgcagg agtggtcatc ggacaggatc
     1381 tgggacagga agatcgccag ggtaatccaa ggcgtgtaga tgcgttctcg ccagacatgg
     1441 ttacagagcc ggcacgcctg ctcgacgaga ggccgcgaga ggagcttgag gaacggcagc
     1501 ccgtgctgct tgccgagttg ctcgcgcagg gaatcgaatt gctggcgtgg gggtgtggga
     1561 gtagcctgag tcatggcgac ctcctgggtg ggtgatcggt gggtgtgtcg aaccaccaga
     1621 gtaccttccc aggaggtcgc gtgactcaac tcccagcagc attccggctt gcgttatcct
     1681 ggtgccattc ggatggaggt cagcacgcgc tggccgaggc agacggctcg gagggcggcg
     1741 ccttgacgta cagcccggcc tgtccctcgg cggccagcat cgcctcatcc
//
LOCUS       DBNGZC010000813         1806 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2222375, whole genome shotgun sequence.
ACCESSION   DBNGZC010000813 DBNGZC010000000
VERSION     DBNGZC010000813.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2222375"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1471)
                     /locus_tag="AC16G0_13940"
     CDS             complement(74..1471)
                     /locus_tag="AC16G0_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_068413152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HIR5100749.1"
                     /translation="MLRDRYDPVDLFALVPQLGLQFEPQLAQLDRLLDDDAIFRRIRT
                     ELARRYPLTPVHGRPSTPVEVILRMLVVMRLYSWSYAQTEYFVHDSLVLRQFCRVYLE
                     KVPDDTVLIRWAQTIGPETLQQLNDRVVQLARSLKVTRGRKLRVDTTAVETNIHYPTD
                     SALLGDGVRVLSRLLRRAKKVLGKAAEALGPETFRSRVRTVRKLAQQLHHIARRKGAE
                     ARDALQAAYGHLIDTAKRTGAQAGRVLEVLVRRTRPPAQRLAQRIGTFLPLLVQGIQQ
                     ASRRVLEGKAVPACQKVLSLFEPHTQIIPRFKAGKPVEFGRKLRLDEVEGGIITGYQI
                     LDQGGGQDQPYLKDSLAAHQRHFGRAPDLLAADRGMASAANEHLARQAGVKRIALPWV
                     GRASPARRQQERERWFRRGYRFRAGIEGRIHVLRRDYGMKRCRYHGERGMGQWVGWGI
                     VTHNLSQIAEAGTNR"
BASE COUNT          306 a          580 c          586 g          334 t
ORIGIN      
        1 acctgggggt tgtgacgagc cggtgcccca tcgcacaagg ggatcggcct ggtcgtctct
       61 catcctcaca tgcttacctg tttgtccccg cttccgcgat ctgggacagg ttatgagtca
      121 cgatccccca accgacccac tgacccatcc ctcgctcacc gtgatagcga caccgtttca
      181 tgccataatc ccgccgcaat acgtggatcc gcccctcgat ccccgctcgg aatcgatatc
      241 cccgccgaaa ccaccgctcc ctctcctgct gtcgtcgggc tggtgaggca cggcccaccc
      301 acggcaaggc gatccgcttc acacccgctt gtcgagccag atgctcgttc gccgccgatg
      361 ccatcccccg atcggcggcc aagagatccg gggcgcggcc gaagtgacgc tggtgcgccg
      421 ccaagctgtc cttcaggtac ggctggtcct ggccgccgcc ttggtccagg atctggtagc
      481 cggtgatgat cccaccctca acttcgtcga gccgcagctt gcggccgaac tccaccggct
      541 tgcccgcctt gaaccggggg atgatctgag tatgcggctc gaacaacgag aggaccttct
      601 ggcaagccgg gaccgctttg ccttcgagga cccgacggga cgcttgctgg atgccttgga
      661 ccagcagggg caagaaggtg ccgatccgct gtgccaggcg ttgagccggt ggcctggtcc
      721 gccgcaccag tacctccagc acccggccgg cctgggcccc ggtgcgctta gcggtatcga
      781 tcaagtggcc gtaggcggcc tgcagcgcat cgcgggcctc ggcccctttg cggcgcgcga
      841 tatggtgcaa ctgctgggcg agcttgcgga cggtgcgcac ccggctgcgg aacgtctccg
      901 gcccgagggc ctcggcggct ttgcccagca cttttttggc gcgacgcagg agtcgcgaga
      961 gcacgcgcac gccatcgccc aacagcgcgc tgtcggtggg atagtggatg ttggtctcca
     1021 cggccgtggt atcgacgcgg agcttgcggc cacgggtgac cttcaggctc cgggccaact
     1081 ggaccacgcg gtcattgagc tgctggagcg tttccggccc gatcgtctgg gcccagcgga
     1141 tgagcaccgt gtcgtcgggg actttctcca ggtagacgcg gcagaactgg cgcagcacga
     1201 ggctgtcgtg gacgaagtac tcggtctggg catagctcca ggaatagagc cgcatgacca
     1261 cgagcatgcg gaggatgacc tccaccggtg tagaggggcg gccatggacg ggggtcaagg
     1321 ggtagcgtcg cgccagctcg gtacggatcc gacggaagat cgcgtcatcg tcgagaaggc
     1381 gatcgagctg agccagttgc ggctcgaact gcaggccgag ttgcgggacc agggcgaaga
     1441 gatcgacggg atcgtagcgg tcgcggagca tggctcgggc ctccggaacg ccaccgcaag
     1501 aggagaattc accgacctcc tatagtctac cgaccgctgc caacctggtc caattcgcac
     1561 cgcaaactag ttctactgtc tcgctaccgt ctgaggttgt tcctcaatgg tggcaacatg
     1621 ttacgcttgc ggtaatgaaa gaaccttgct tgttcgttgc gtcgtagcca gcgggtgctg
     1681 cggcggcaaa gtgacaaggg gtgattcgtg ttgaggtggg actcgatcaa cccaccgatc
     1741 agttgccgaa gctgcgggaa ggtcagtgcc ggggtccgga ttttttcccc gccgcgtctc
     1801 ctgatt
//
LOCUS       DBNGZC010000814         2821 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_265365, whole genome shotgun sequence.
ACCESSION   DBNGZC010000814 DBNGZC010000000
VERSION     DBNGZC010000814.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_265365"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..174)
                     /locus_tag="AC16G0_13945"
     CDS             complement(13..174)
                     /locus_tag="AC16G0_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100750.1"
                     /translation="MTVLLPESTPQELKCIVTAEGRVQLFCLRHLANGIVLRPFPGQY
                     RLWADPTAS"
     gene            complement(237..941)
                     /locus_tag="AC16G0_13950"
     CDS             complement(237..941)
                     /locus_tag="AC16G0_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5100751.1"
                     /translation="MLRVEKLEVAYGRLQALWGVSFDVREGEIVALVGANGAGKTTTL
                     KTLSGLLHPKGGGIWLGAERVDHRSPAEIAEMGVVHVPEGRKLFPGMSVLENLLMGSY
                     PRPARPHRQRRLEDVFTIFPTLSERQRQMAGTLSGGEQQIVAIGRGLMAGPKVLMLDE
                     QSLGLAPIMVAEMFRVIAEINRKGVTVLLVEQNTEHALALAHRGYVLESGRVVLAGTG
                     QELLASSQVREAYLGL"
     gene            complement(943..1662)
                     /locus_tag="AC16G0_13955"
     CDS             complement(943..1662)
                     /locus_tag="AC16G0_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020588874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5100752.1"
                     /translation="MPLLDVSDLRKDFGGLRAVSALSFVVEAGEILGLIGPNGAGKTT
                     VFNLITGYLRPTTGDIRLEGRSLVGLKPHAVTRRGIARTFQIVKPFPKLSVQENATLA
                     AFMRLPARRDAEAEARRVLDTVGLSAKLASPASDLTLAEQKRLEMARALATRPQLLLL
                     DEPMGGLNPKEVEDASKLVRQIRDAGVTVILVEHVMKAIMRISHRVVVIHHGEKIADG
                     PPDRVVRDTGVISAYLGERVT"
     gene            complement(1649..2599)
                     /locus_tag="AC16G0_13960"
     CDS             complement(1649..2599)
                     /locus_tag="AC16G0_13960"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5100753.1"
                     /translation="MRSAAATGAIVLLLAIPFVTHNQFLLHLGITVLLWTVLGSAWNL
                     LGGYAGQVSFGHATFFGVGAYVTMILYLKLGLAPWYGMVVGGMLAALASLPIGYLCFR
                     LRGPYFSLATLAVAEIVRLVALNWEAVTNGPVGLLITTLPAVRFGGRPVNWEEQSPFF
                     YLVALLAVMAMLVTYWLSRSRLGAYLLAIREDEDTAESIGIDTVTTKVTVLALSAFFA
                     GLGGGFYGMYYRYEDPDAVFPIALSVEMVFMAVVGGLATVEGPVIGAIFLVTIGELFR
                     ERFLVGHLIFYGLFMMLTIRYLPEGIWGGIRRAFSSHASA"
     gene            complement(2596..>2821)
                     /locus_tag="AC16G0_13965"
     CDS             complement(2596..>2821)
                     /locus_tag="AC16G0_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5100754.1"
                     /translation="PTVGEILVVKCFVIVVLGGLGSVSGAIAGGVILGLVESLGAVYV
                     SVAYKDTIGFIIFLLVLLFRPSGLLGVGKS"
BASE COUNT          557 a          944 c          893 g          427 t
ORIGIN      
        1 gtgcttccgg cgctacgacg cggtcggatc cgcccagagg cgatactgcc ccgggaaggg
       61 ccgcaacaca atcccgtttg ccagatggcg caggcagaag agttggaccc tgccttccgc
      121 tgtgacgatg cacttcagct cttggggggt cgactcgggt aggaggacag tcatgcatgc
      181 agaggatcga ggtttggaag cggcggggaa tctatcggaa cactgatggc atgtcgctac
      241 aagcccaggt acgcctcgcg gacctgggag ctggccagga gttcctggcc tgtgccagcc
      301 agaaccaccc ggccggattc caggacatag ccccggtggg ccaaggcgag ggcatgctcc
      361 gtgttctgct cgaccaagag gaccgtgaca ccctttcggt tgatctccgc aattacccgg
      421 aacatctccg ccaccatgat gggcgccagc cccagcgact gctcgtccag catcagaacc
      481 ttcggtcccg ccatgagccc ccggccgatg gccacaatct gttgctcgcc gcccgagagc
      541 gtacccgcca tttgccgctg ccgctcggag agcgtcggga agatggtgaa cacgtcttcc
      601 agtcgacgct gccggtgggg tcgagcgggt cgcgggtagc ttcccatcag cagattttcg
      661 aggacactca tccccggaaa gagctttcgt ccttccggga cgtggaccac gcccatctcc
      721 gcgatctctg cgggggagcg atggtcgacc cgctcggcac caagccaaat gccgcctcct
      781 ttcgggtgga ggaggcccga cagggtcttc agcgtcgtgg tcttgcccgc cccgttggcc
      841 ccgaccagcg cgacgatttc cccctcgcgg acgtcgaagc tcacgcccca cagggcctga
      901 agcctcccat aggccacctc gagcttctcc acgcgcagca tgctaggtca cccgctcgcc
      961 gagataggcc gagatgacgc cggtgtcccg gaccacccgg tcgggagggc cgtcggcgat
     1021 cttctcgccg tgatggatca ccaccactcg atgcgagata cgcatgatgg ccttcatcac
     1081 gtgctccacc aggatcacgg tgacgccggc atcccgaatc tgcctgacca gcttcgaggc
     1141 gtcctccacc tccttcgggt tcaggccccc catgggctcg tccaggagga gaagctgggg
     1201 gcgggttgcc agcgcccggg ccatctccag acgcttctgc tccgccagcg tcaggtcgga
     1261 tgccgggctc gccagcttgg ccgagagacc taccgtgtcg aggacgcggc gtgcctccgc
     1321 ttcggcatcg cgtctggccg ggaggcgcat gaaggcggcc agggtcgcat tttcttgaac
     1381 gctcagcttg ggaaacggct tcacgatctg gaaggtacgt gcgataccgc ggcgagtcac
     1441 ggcgtggggc ttcagaccga cgaggctcct cccttccaga cggatgtccc cggtcgtcgg
     1501 gcgcaggtac cccgtgatca ggttgaagac cgtggtcttg ccggccccgt tgggaccgat
     1561 gagtcctagg atttccccgg cctccaccac gaacgacagc gcggagacgg cacggagccc
     1621 gccgaagtcc ttgcgcagat ccgaaacgtc aagcagaggc atggctggag aaggcccgac
     1681 gaatcccgcc ccagatgccc tcgggcaggt agcggatcgt cagcatcatg aagaggccgt
     1741 agaagattag gtgacccact aggaaccgct cccggaacag ctcaccgatg gtgaccagga
     1801 agatcgcgcc gatcaccggt ccctccaccg tcgccagacc accgaccacc gccatgaaga
     1861 ccatctccac cgagagagcg atgggaaaga ccgcatccgg atcctcgtag cgatagtaca
     1921 ttccataaaa acccccgccc agccccgcga aaaaggcgga cagcgccagg acggtgacct
     1981 tggtcgtcac ggtatcgatg ccgatggatt cggccgtgtc ctcgtcttcc cggatggcca
     2041 ggaggtacgc gcccaggcgc gagcgcgaca gccagtaggt gacaagcatg gccattacgg
     2101 ccagcaacgc gaccaggtag aaaaacggcg actgctcctc ccaattcacc gggcgaccgc
     2161 cgaatcgaac agccggcagc gtggtgatca gtagacccac cggtccgttg gtgacggcct
     2221 cccagttgag ggccaccagg cggacgatct cggccacggc caaggtcgcc agggagaagt
     2281 acggcccccg cagccggaag cagaggtatc cgatgggaag ggacgccagg gcggccagca
     2341 tcccgcccac gaccatcccg taccaggggg ccaggcccag cttgaggtag aggatcatgg
     2401 tgacataggc gcccacaccg aagaaggtcg cgtgtccgaa ggagacctgt ccggcatagc
     2461 cgccgaggag attccaggcg ctgcccagca ccgtccagag gagcacggtg atgcccaggt
     2521 ggagcaggaa ctggttgtgc gtgacgaagg ggatcgcgag gagcaggacg atcgccccgg
     2581 tcgcggcggc ggacctcatg atttccccac ccccagaaga cccgacggcc ggaaaagcag
     2641 caccaggaga aagatgatga agccgatggt atccttgtag gccacggaga cgtacacggc
     2701 ccccagggac tccaccaggc ccaggatgac cccgccggcg atggcccccg acaccgatcc
     2761 caggccgccc aggaccacga tgacgaagca cttgaccacc aggatctccc cgaccgtggg
     2821 a
//
LOCUS       DBNGZC010000815         1926 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1045047, whole genome shotgun sequence.
ACCESSION   DBNGZC010000815 DBNGZC010000000
VERSION     DBNGZC010000815.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1045047"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="AC16G0_13970"
     CDS             complement(<1..418)
                     /locus_tag="AC16G0_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR5100755.1"
                     /translation="MDFPILDLMDQDGCYHKLLDLLHPGGLVCPRCAARDGLNIHRHR
                     PESAVVDYRCKGCRRVFNLFTGTPWQGTHLSPSQILLILRGIAQGVPTAKLAREVGVS
                     RQDLLRLRHTIQARALTTADRSPLPDDQAEADEMYQN"
     gene            514..684
                     /locus_tag="AC16G0_13975"
     CDS             514..684
                     /locus_tag="AC16G0_13975"
                     /inference="COORDINATES: protein motif:HMM:NF013921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR5100756.1"
                     /translation="MSQVFYGRLRRQIGPIFRDLCRQRGIDLVEGHAMPDHTHLCVSI
                     PPKYGGRTRSAS"
     gene            complement(882..>1926)
                     /locus_tag="AC16G0_13980"
     CDS             complement(882..>1926)
                     /locus_tag="AC16G0_13980"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009673839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HIR5100757.1"
                     /translation="KRYLHRFIHEFPRISTLAGVDRTPYNQYDSIIRPITEWLKGQGV
                     LFKMRSRVTSLEFKPGTGQSTVQRIQYIHDGQPHEIALAPGDYVFVTNGSMTADSMLG
                     TMTTPAPMKTDPRADGAWALWEDLAKERPEFGHPSVFDGRVAESYWPSFTVTLRDPTF
                     LRLEREFTGNAPGTGGLVTFVDSNWLMSIVVAYQPHFRDQPDGVQVFWGYGLCPDRPG
                     NYVPKTMTECTGAEILTELCSHLGFTEQLPRILETSICIPCTMPFVTSQFLTRARGDR
                     PPVVPQGSTNLAFVGQFCEIPDDVVFTVEYSVRSAMTAVYSLLHLEQEPPPMYKGEHD
                     PRVLFDAIVTALR"
BASE COUNT          359 a          580 c          648 g          339 t
ORIGIN      
        1 cgttctggta catctcgtcg gcctcggcct gatcgtccgg cagtggcgag cggtccgcgg
       61 tggtcagggc gcgggcctgg atcgtgtgcc gcagccgcag cagatcctgg cggctgactc
      121 ccacctcacg ggcgagtttc gcggtgggca ccccctgcgc gatgccccgg aggatcaaga
      181 ggatctggct ggggctgagg tgggtaccct gccagggggt gccggtgaac aggttgaaga
      241 cccggcggca gcctttgcag cggtagtcaa cgaccgcaga ttcggggcga tggcgatgga
      301 tgttcagccc gtcccgggcg gcgcaacggg ggcagaccag gccgccggga tggagcaggt
      361 ccaggagctt gtggtagcaa ccatcttggt ccatcaggtc gaggatcggg aagtccatca
      421 ggtcaactcc caggggatga tcgtcaatac acccaatggc caagagaaca gaacggcaga
      481 tggctgggaa gggccaacca ccaaattggt tacatgagcc aagtcttcta cggaagactg
      541 cggcggcaga tcggcccgat ctttcgggac ctgtgccgac aacgagggat cgacctggtg
      601 gaagggcacg cgatgcccga ccacacccac ctgtgcgtga gcatcccgcc caagtacggc
      661 gggcgtacac gatcggcttc ctgaacggga agagtgtggt ttggatccat cgggagttgc
      721 tccacgagcg tcggatgacg gggctgcact tctgggcagc ggggtatggc ccctcgggag
      781 ggctttctca taccacagcc tctggccgtg gatccttatt gggagagtga gtagttcgac
      841 tatcccgatt ggggtccgtt gacacgctcc gagtggcacg cctaccggag tgccgtcacg
      901 atggcgtcga acaacacacg gggatcgtgt tcgcccttgt acatcggcgg cggctcctgc
      961 tccagatgaa ggagggaata cacggccgtc atcgccgacc gcacggaata ttcgacggta
     1021 aacaccacat cgtccgggat ctcgcagaac tgcccgacga aggcgagatt agtggaccct
     1081 tgtggcacca ccggcggccg atcacccctt gcgcgcgtca ggaactggct ggtgacgaag
     1141 ggcatcgtgc acgggatgca gatggacgtc tcgaggatgc gaggcaactg ctcggtgaag
     1201 cccagatgag agcagagctc cgtcaggatc tccgcaccgg tgcattcggt catggtcttc
     1261 ggcacgtaat tcccgggccg atcaggacaa aggccgtagc cccagaagac ctgcaccccg
     1321 tccggctggt cgcggaagtg cggctgatag gccaccacaa tggacatgag ccagttcgaa
     1381 tccacgaagg tcaccaggcc gcccgtcccc ggcgcattcc cggtgaactc ccgctccaag
     1441 cggaggaagg tcggatcgcg tagcgtcacc gtgaaggagg gccaatagga ctccgcgacg
     1501 cgcccgtcga acacggacgg atggccgaac tcgggccgct ccttcgcgag gtcctcccat
     1561 aacgcccacg cgccgtccgc cctagggtcc gtcttcatgg gggcgggtgt cgtcatcgtg
     1621 cccagcatgg aatccgctgt catcgatccg ttggtgacga acacgtagtc gcctggcgcg
     1681 agtgcgatct cgtgcggctg cccgtcgtgg atgtactgga tgcgctggac cgtgctctga
     1741 cccgtccctg gcttgaactc caggctcgtg acccggcttc gcatcttgaa gaggacgccc
     1801 tgccccttga gccattccgt gatgggccgg atgatcgagt cgtactggtt gtacggggtg
     1861 cgatccacgc ccgccagcgt gctgatacgt gggaactcgt ggatgaaccg gtggagatag
     1921 cgcttg
//
LOCUS       DBNGZC010000816         4178 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1932892, whole genome shotgun sequence.
ACCESSION   DBNGZC010000816 DBNGZC010000000
VERSION     DBNGZC010000816.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4178)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4178)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4178
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1932892"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..1239)
                     /locus_tag="AC16G0_13985"
     CDS             complement(229..1239)
                     /locus_tag="AC16G0_13985"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5100758.1"
                     /translation="MLAVVTGGTGFLGSHLVRLLCESRADVRVLLRPGSPPGSVPPGV
                     EVRVGDIRDRDALMEAFAGAATVFHLAGLTAPWGPRHRYLEINLEGTEAVLDACLAAR
                     VARLVHVSSLVVLGIECDRRGLTEEAPYATAFVSPYEESKVRAEQLVLERARATGLPT
                     VVVRPGMGWGPRERVFLPTLIRAMGSPLFFMVGNGRNTLDLSYARNVAHALWLAGTHP
                     EAVGRVYNLADGSGTTCREYLTTLARALGLPVPRLRLPRGLVQFLMRVALPPEPEPKG
                     MVLRPSQASLLRLCALFRDSEPDTSRIRKELGYVPPVDFATGVAETVDWYRQTYQDQR
                     VG"
     gene            complement(1267..2628)
                     /locus_tag="AC16G0_13990"
     CDS             complement(1267..2628)
                     /locus_tag="AC16G0_13990"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR5100759.1"
                     /translation="MEVEATMSRHVLLVEDEPILRLTLANDLAEEGYEVTAASDGTEG
                     LSRLRERSFDVTLLDLKLPKVDGLTLLRSFKAANPQGFAVMMTAYGTIQSAVAAMKAG
                     ASDYLLKPFSTEELLLLLRGLFAQRGDLPSAETKANGVRRFGDLIYASPKMARVCDLI
                     ATVARSDATVLIQGETGTGKELAARALHEYSGRRAQPMIRVSCAAIPETLLETELFGH
                     EKGAFTGAIRERRGRFELAHGGSLFLDEVADLGPAVQAKLLRVLQEKEFERVGGTRTI
                     KVDVRLISATGKRLEDEVLAGRMREDLFYRLKVITIRLPALRERKEDILPLAEHFLEK
                     FRRPLNKEIRGLSPEVCRSLHAYPWPGNVRELEASIQRAVTLTKGKLLTPEDLSFEDA
                     LTSDDQTNVSTHPLTDAIREAERRYLQEVLQSVGGQRLRAAEILGISRKTLWKKLKLL
                     GLE"
     gene            complement(2618..4069)
                     /locus_tag="AC16G0_13995"
     CDS             complement(2618..4069)
                     /locus_tag="AC16G0_13995"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5100760.1"
                     /translation="MTLRFRMILGIGTILFIVILFYSFIALRSQGNYLQGLAKHEADL
                     IAAVADRALARAMGQGETEVVQAILSRIGEHAVLAGIRIVDTTGRIRRSNRTDEIGRL
                     LPPGERPVGNAPAPVWDYREQTVGSFRPILNGPTCTGCHPRDRATLGFLNVRVSFPAI
                     DSEVSQQWSFMILMAVVSLVAAGALIAIFFTFFLGRRIDALSYTMSRVEAGDLAARVP
                     EDTPDELGLLGRSFNAMVARLADARRQLEDRHAEEIRRAQNLASLGQMAAGIAHEINN
                     PIAGMQNCVRTLLKGTRDEAQRAQYLDMLREGLGRIGRTVGQLLNFARESKPRLTQTD
                     LPPLLQRCLVLLEHDLAVRRVSFRLALDGKLPVLMVDPHQLEQVFLNVLKNALEAMPD
                     GGSLTVSAGLQPRQTGPFVEVRIADSGVGIPAENLHRIFDPFFTTKEVGEGTGLGLSV
                     SYGIVRAHGGIIDVESDVGKGTTVTIALPLNGG"
BASE COUNT          741 a         1372 c         1331 g          734 t
ORIGIN      
        1 cgggaggtca cctcgatctg gatggactca cagggtcgcc ctcgggtccg tcgtttgagc
       61 cagtaggcga ggcgtctgga cattgatggc tagctcacag gctggtggca ggaccaagca
      121 acgatcaacg attcgagggt tcgacagttc gaccggacag cgggcgtgcc agcacctgga
      181 ctgccatact gccgaatcgc cgaaccttct tgagtcacca tcccctcgtc agcccactcg
      241 ctggtcctga taggtctggc ggtaccagtc tacggtctcg gccaccccgg tcgcgaagtc
      301 gaccggaggc acgtacccca gttctttgcg aatccggctg gtgtccggct cgctgtcccg
      361 gaagagggca cacaggcgta gcagggatgc ctgagacggg cggagaacca tgcccttcgg
      421 ttcgggctcc ggcgggagcg ctacccgcat gagaaactgc accaagccgc ggggcaggcg
      481 gagccgggga accggcaggc ccagcgcccg tgcaagcgtg gtcagatact cacggcaggt
      541 ggtgccggaa ccatcggcaa ggttgtaaac acgtccgacc gcctcgggat gggtgccggc
      601 cagccagaga gcgtgggcca cgttccgggc gtagctcagg tccagcgtgt tccggccgtt
      661 gccgaccatg aagaaaagcg gcgagcccat ggcgcggatc aaggtgggca ggaacacgcg
      721 ctctcgaggt ccccacccca tgcccggccg taccaccaca gtcggcagac ccgtcgcccg
      781 cgctcgctcc aggaccagtt gctcggcccg taccttgctt tcctcgtacg ggctgacgaa
      841 ggccgtcgca tacggggcct cttcggtgag gccgcgccgg tcgcactcga ttcccaggac
      901 caccaggctg ctcacgtgga cgaggcgggc tacccgggcc gccaggcacg catccaggac
      961 tgcctctgtg ccctccagat tgatctccag gtagcggtgt ctcgggcccc agggggccgt
     1021 gagcccggcc aggtggaaga ccgttgcagc cccagcgaag gcctccatga gggcgtcccg
     1081 atcgcggatg tccccgaccc ggacctcgac cccgggtgga acagatccgg gtgggctccc
     1141 gggacggagg agcacgcgaa catctgcccg gctctcgcag agcagacgta ccaggtggga
     1201 tcccaggaac ccggttcctc ctgtcacgac ggcgagcatc cgacgagtgc tccgagtcgg
     1261 gctgacctac tccagcccga gcaacttcag tttcttccag agggtcttcc ggctgatccc
     1321 cagaatctcc gccgcacgca ggcgttggcc gccaacgctc tggaggacct cctggaggta
     1381 gcggcgctct gcctcccgga tggcgtctgt gagggggtgg gtggagacat tcgtctgatc
     1441 gtcggatgtc agggcgtctt cgaatgacag atcctccggc gtgaggagct tccccttggt
     1501 cagggtcacg gcccgctgga tgctggcctc caattctcgg acgttgccgg gccaggggta
     1561 ggcatgaagg ctccggcaga cctccgggct cagcccgcgg atctccttgt tcagcggccg
     1621 acgaaacttt tccagaaagt gctccgcgag gggaaggatg tcctcctttc tctcccggag
     1681 cgcaggcagt ctgatggtga tgactttcag gcggtaaaaa aggtcctccc gcatccggcc
     1741 ggccaggacc tcgtcctcca accgcttccc cgtggcggag atcaaccgga cgtccacctt
     1801 gatggtgcgg gtgcccccga cccgctcgaa ctccttctcc tggaggaccc gcaggagctt
     1861 ggcctggacc gccggtccca ggtcggctac ctcgtcaagg aaaagactcc ccccatgagc
     1921 cagctcgaag cgtcctcgcc gctcgcggat ggcccccgtg aaggcgccct tttcgtgacc
     1981 gaacagctcc gtctccagca acgtctccgg gatcgcagcg caggacaccc ggatcatggg
     2041 ctgcgcccgc cgcccactgt attcgtggag ggctcgggct gccaactcct ttcccgtgcc
     2101 cgtctcaccc tggatcagaa ccgtggcgtc gctccgtgcc acggtggcga tgaggtcgca
     2161 cacccgggcc atcttcggac tggcgtaaat gagatcgcca aatcgccgga caccgtttgc
     2221 tttcgtctct gcagacggga ggtctccacg ctgggcaaag aggccccgga gcaggagcag
     2281 gagctcctcg gtggagaagg gcttcagtaa atagtcgctg gccccggcct tcatggccgc
     2341 cacggccgac tggatggtcc cgtaggcggt catcatgacg gcaaagccct gagggtttgc
     2401 agccttgaaa ctcctgagga gggtcagtcc gtccaccttg ggcagcttca gatccaggag
     2461 agttacatcg aagctccgct cccggagacg gctcagaccc tcggttccgt cggaggcggc
     2521 ggtgacctcg tagccctctt ccgcgagatc gtttgccagg gtcaaccgga ggataggctc
     2581 atcctccacc aagaggacgt ggcggctcat cgtggcttca acctccattc agggggagag
     2641 caatcgtcac ggtcgttcct tttccgacat cggactcgac atcgatgatc cctccgtgcg
     2701 ccctcacgat cccgtagctc accgagagtc ccagaccggt cccctctccc acctccttgg
     2761 tggtgaagaa tgggtcgaag atccggtgga gattctccgc cggaattccc acgccgctgt
     2821 cggcaatacg gacctcgacg aaggggcccg tctgccgcgg ctgtaacccg gcgctgaccg
     2881 tgaggctgcc cccatccggc atggcttcca gggcgttctt caggacgttc aggaagacct
     2941 gctccagttg gtgcggatcg accatgagga cggggagctt gccgtccaac gcgagcctga
     3001 aggacacccg tcgaaccgcc aggtcgtgct ccagaagcac gaggcagcgc tggaggaggg
     3061 ggggaaggtc cgtctgcgtc aggcgaggct tggattcccg ggcgaagttc aagagctgac
     3121 cgacggttcg cccgatccgg cccagccctt cacgcagcat gtccagatac tgcgcccgct
     3181 gcgcctcatc cctcgttccc ttcaggaggg tccggacgca gttctgcatg ccggcaattg
     3241 gattgtttat ctcatgagca ataccggctg ccatctgccc cagggatgcc agattttggg
     3301 cccgccggat ctcctcggcg tggcggtcct ccaattgacg ccgggcgtcc gcaagcctgg
     3361 ccaccatggc gttgaagctt ctccccagga gacccagttc gtccggggtg tcctcgggca
     3421 ccctggccgc gagatccccg gcttccactc ggctcatggt gtaagacagg gcatcgatcc
     3481 ggcgccccag gaagaaggtg aagaagatgg cgatcaatgc gccggcagcc acgagagata
     3541 cgacagccat gaggatcatg aatgaccact gctgcgagac ctccgaatcg atggccggga
     3601 aggacacccg cacattgagg aaaccgaggg tcgcgcgatc ccggggatgg cagccggtgc
     3661 acgtcggacc gttgaggatc ggcctgaagg aaccgaccgt ctgttcgcgg tagtcccaaa
     3721 ccggcgccgg ggcgttgcct actggccgct ctccgggagg caagagccgg ccaatctcgt
     3781 cagtccggtt ggagcggcga attctccccg tggtatccac gatccggatc ccggccagga
     3841 ctgcgtgctc gccgatgcgt gaaaggatgg cctggacgac ctccgtttcg ccttggccca
     3901 tggctcgagc cagtgcgcga tctgccacgg cagcgatcag gtcggcttcg tgcttcgcca
     3961 acccctggag ataattcccc tggcttcgca gggcgatgaa cgagtagaag agaatgacga
     4021 tgaagaggat cgtcccgata cccaggatca tcctgaatcg aagggtcatc ggctcctcac
     4081 agaaccagtc agggttcagc ttaagactga gtactgactg ccgggtgctc agcgctttcg
     4141 ccctcgccag cctcgatatg ccgcttgatc gcctggag
//
LOCUS       DBNGZC010000817         1881 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2418092, whole genome shotgun sequence.
ACCESSION   DBNGZC010000817 DBNGZC010000000
VERSION     DBNGZC010000817.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2418092"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="AC16G0_14000"
     CDS             <1..1260
                     /locus_tag="AC16G0_14000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5100761.1"
                     /translation="VIIGASAAGLAALEAIREVDKECAVTILSREKAPLYSRVGLTHF
                     LDGTVKVSGMNTHGPEYFERLKATLLAGVAAVQVDPEAHTVKLSNRKSLKYDTLLVAS
                     GSSAVIPPIPGVKLRGVYPCITRNDAVAIDRAVAGARQVAVIGAGLIGIQAVDALAKR
                     KKKVTVIEQMPHVMPAMVDPTAARLFEDLLREKGHTVRTGIRATELLGKSGRLAGVLL
                     ENEEVIPCQLLIMAVGVRPNVEFLERTKIQKRQGLLVNEHQQTSLKGIYAAGDVAETI
                     DMFSGQRVLIAIWPEALNQGRVAGLNMAGVPTHYEGSMAMNTTSVLGLPIASLGLWQV
                     TDTADYRIHTLLQEAKREYRKLVFQDGKLVGAILVGPGMNAEAGILHNFIRSRQSFSV
                     SPEQLVTGPISWGRVLRDNRLAGRAAA"
     gene            1369..1641
                     /locus_tag="AC16G0_14005"
     CDS             1369..1641
                     /locus_tag="AC16G0_14005"
                     /inference="COORDINATES: protein motif:HMM:NF025807.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR5100762.1"
                     /translation="MKIQVALYATLSQYLPAGAENRKATIEVRDTATVRDVLTQLGIP
                     QDLPNILLVNGRQAPEDTVLKDGEVVSVFPPLAGGVDVPGSHISPS"
     gene            1706..>1881
                     /locus_tag="AC16G0_14010"
     CDS             1706..>1881
                     /locus_tag="AC16G0_14010"
                     /inference="COORDINATES: protein motif:HMM:NF018682.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HIR5100763.1"
                     /translation="MPARLGVIADDLTGANDTGVQFARHGARTLVPLDWHDLAALGRA
                     ADVLVLNTNSRGLSA"
BASE COUNT          348 a          590 c          619 g          324 t
ORIGIN      
        1 gtgatcatcg gcgccagcgc cgccgggctg gcggcgctgg aggcgatccg cgaagtggac
       61 aaggagtgcg cggtcacgat cctctcgcga gagaaggcgc cgctctactc ccgcgtgggg
      121 ctcacgcact tcctggacgg gacggtgaag gtatccggga tgaataccca cggcccggag
      181 tatttcgaac gactgaaggc gacccttctg gccggggtgg cggccgtcca ggttgaccca
      241 gaggcccata cggtgaaact gtccaaccgg aagagcttga aatatgacac gctcctcgtg
      301 gccagcggct cctccgccgt gattcccccg atcccgggag tgaagctccg gggggtttat
      361 ccctgcatca cccggaacga cgccgtggcc atcgaccggg ccgtggcggg agcccgtcag
      421 gttgctgtga tcggcgcggg gctcatcggg atccaggctg tggatgccct ggccaagcgt
      481 aagaagaagg tcacggtcat cgagcagatg ccgcacgtga tgccggccat ggtcgacccg
      541 acggcggccc gcctcttcga ggacctcttg cgggagaaag gtcacaccgt ccgcaccggc
      601 atccgggcga cggaactcct gggcaagtcc gggcggcttg caggcgtgct gctggaaaac
      661 gaggaggtca ttccctgcca gcttctcatc atggccgtgg gggtccggcc caatgttgag
      721 tttctggaac ggacgaagat tcagaagcgg caggggctgc tcgtgaacga acaccagcaa
      781 accagcctca agggcatcta cgcagcgggg gatgtggcag agaccatcga catgttctcc
      841 ggccagcggg tgctcatcgc catctggccc gaagcgctca accaggggag ggtggctggc
      901 ctgaacatgg cgggggttcc gacgcactac gagggctcca tggccatgaa tacgacctcc
      961 gtgctggggc tgcccattgc atccctgggc ctatggcagg tgaccgacac cgccgactac
     1021 cgaatccaca cgctgcttca ggaggcgaag cgcgaatacc ggaagcttgt cttccaagat
     1081 gggaagctgg tcggcgccat cctggtgggc cctggcatga acgcagaagc cgggatcctg
     1141 cacaacttca tccgcagccg ccaatccttc agcgtgagcc cggagcaact cgtgaccgga
     1201 cccatctcct ggggccgagt cctgcgcgac aaccggctgg ccggccgtgc cgccgcctga
     1261 ggactcgccc aaggggaatt gagacaccga caaccgtcaa gcggacattc gggaacattg
     1321 atcggttgac ggatttcatt ttttcattga ttcccttgga gggttcgtat gaaaatccag
     1381 gtggcactgt atgcgacgct gtcgcagtac cttccagcgg gcgcggagaa ccggaaggcg
     1441 accatcgagg ttcgcgacac ggccacggtg cgtgatgtgt tgacccaact cgggatccct
     1501 caggatttgc caaacattct cctggtgaat ggcagacagg cccccgaaga tacggttctg
     1561 aaggacggcg aagtggtctc ggtctttcct ccactcgcgg ggggcgtgga cgtgcccggt
     1621 tcccatatca gtccctcgta acgcatcagc ggccgaccgc tcggctgcat cctgccagga
     1681 gccgcgcctt tgcgggggag aggccatgcc tgcccgtcta ggcgtcatcg ccgacgatct
     1741 cacgggagcc aacgataccg gggtccagtt tgcgcggcat ggcgcccgga ccctcgtgcc
     1801 gctggactgg cacgacctgg ccgcgttggg tcgggccgcc gacgtcctcg tccttaacac
     1861 caacagccgc ggattgtcgg c
//
LOCUS       DBNGZC010000818         4579 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_558380, whole genome shotgun sequence.
ACCESSION   DBNGZC010000818 DBNGZC010000000
VERSION     DBNGZC010000818.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4579)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_558380"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="AC16G0_14015"
     CDS             complement(<1..629)
                     /locus_tag="AC16G0_14015"
                     /inference="COORDINATES: protein motif:HMM:NF014073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR5100764.1"
                     /translation="MWDQILAAVLVNLQPSVLFVVAVGTIVGTIIGALPGLSAVSGVA
                     LMLPFTFTMEPAKGLIMLAAVYQSAEYGGSISAILINTPGTSGAACTILDGTPLTQKG
                     QAQEALYTSLLAGTVGGAFGGLVLLFFTPVLADLSLFLGPAEIFWVAVAGMSLVASLS
                     GRHIFKGLIGVLLGVALTIVGQDAISGDMRFTFDIYQLVSGIPLVPSLLG"
     gene            complement(671..1675)
                     /locus_tag="AC16G0_14020"
     CDS             complement(671..1675)
                     /locus_tag="AC16G0_14020"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR5100765.1"
                     /translation="MTLKKSILLRVLVALWLTTLLLGAAPAFAGESSAIAKPAGYPSR
                     PVEFIVPFDPGGGADASQRVFNKYAEPLIGQRLVIVNKPGAGGATGWAEMVRAKPDGY
                     TLTITTPPFNIIPALVRPKQTGYKMDQFTNICIYAIVPDLLYAREGGQFKTLKDVVEY
                     AKQNPEKTKAANTGALGADFMTTLLIENATGTKFTQIPFTGGAQALQATLAGTTDVMV
                     GTSLYAMAQKGKLRPLAIATDQRDSKLPEVPTFKELGYDVFSERYRSLGGPQRLPGEI
                     INYWGQVCKQVVDNPQFQSEMDKIGQPAGYRGPAEAQQAIDKMTKDMQALVEKYSLAK
                     "
     gene            complement(1730..1948)
                     /locus_tag="AC16G0_14025"
     CDS             complement(1730..1948)
                     /locus_tag="AC16G0_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100766.1"
                     /translation="MAATWFSAGLRIYDISDPFRPEEIGAFLPETPPGQRACRISDVF
                     VEDRNVIYAADRARGGLYILEYTGRPLN"
     gene            complement(2042..2971)
                     /locus_tag="AC16G0_14030"
     CDS             complement(2042..2971)
                     /locus_tag="AC16G0_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HIR5100767.1"
                     /translation="MTNVPPVRIARNLKLIGHSDLGGAPNAGEGMGVKITRDGRRILY
                     IAHENPPMAFSILDVTDPSRPTLLWQLPVPHADARANSLALRNDLLLLAYQCKTPGQK
                     PAGFQIYDVSNPVKPREVAFFDVSGPYSQGVHFVFLTDGRFAHISTGAPDFEPSHPKD
                     HQFYMIVDLENPSKPREVGRWWMPGQRKGDKESLPPRHTAPSLDFAFRLHNAQCYPER
                     PDRAYLGYIDGGIVILDIADKGRPRQISRLDYHPPFPGFTHTVLPLFDRNLLVVTDEA
                     TGDEGIDWPKRLWMVDAREETNPVMISSFPTPE"
     gene            complement(3038..3892)
                     /locus_tag="AC16G0_14035"
     CDS             complement(3038..3892)
                     /locus_tag="AC16G0_14035"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5100768.1"
                     /translation="MTGIIDFRLRPPAGGFLTTIMFREKERTMARTRQLSLEPPPSLL
                     ELSTEKLLAEMDAAGVEIGVITGRNTGSGFGTVTNEEVVAIAAGYPGRFVACGGVNPV
                     DTAAALREVDRIAGDWRMPAISMEPGFAPQPMRANDRRLYPIYARCADRGLAVILTLS
                     LMTGPDVSYCLPVDVDQVAHDFPGLTLVIAHACWPWVQPMVGVAFRRTNVYLSPDIYA
                     INLPGREDFALAARTYLQDRFLFASGYPFAPVGGAVQAYRAMGFSGEIADKIFRRNAA
                     ALLRLTDR"
     gene            complement(3889..>4579)
                     /locus_tag="AC16G0_14040"
     CDS             complement(3889..>4579)
                     /locus_tag="AC16G0_14040"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5100769.1"
                     /translation="LEGKQELVAELEGRVGPETILASNTSGLPITQIAARARRPQRVV
                     GMKFVNPAHLVPLVEVVKGDATAPEIVTQACGLLRRIGKRPVAVMRDVPGFLNNRIQQ
                     AMRREAISLVARGIASAEDVDAAVRWGFGFRLLGAGTLETMDLVGLEQILKIHEYIIP
                     DLEAAPEPSPLLRKLVARGDTGARAGRGFLTWDAERERAARARMEALLIAALKLVRQL
                     ERTDGPQGGIA"
BASE COUNT          763 a         1505 c         1528 g          783 t
ORIGIN      
        1 cccagcaggg aggggaccaa ggggatgccc gagaccaact ggtagatatc gaaggtgaac
       61 cgcatgtccc cgctgatggc gtcctgcccg acgatggtca gggcaacccc caggagcacg
      121 ccgatcaggc ccttgaagat gtgccggcca gaaaggctcg ccaccaagga catgcccgcc
      181 acggcgaccc agaagatctc ggccggcccc aggaacaggg acagatcggc caggacgggg
      241 gtgaagaaca gcagcaccag acctccaaag gctccaccca ccgtcccggc cagcagcgat
      301 gtgtacaggg cctcctgggc ctgccccttc tgggtgaggg gagtgccgtc gagaatcgtg
      361 cacgctgccc cggaggtgcc gggggtattg atcagaatcg ccgagatgga gccgccgtac
      421 tcggccgact ggtagacggc ggcgagcatg atcaggccct tcgccggctc catggtgaag
      481 gtgaagggga gcatgagggc caccccgctc accgcgctga ggcccggcag ggcgccgatg
      541 atggtcccga cgatggtccc gaccgccacg acgaacagca ccgacggctg cagattgacc
      601 aggacggccg ccaggatctg atcccacata tgaggtcctc cggtctccgg ggccaggtca
      661 aggcgtctgc ctatttcgcc aggctgtact tctccaccag ggcctgcatg tctttggtca
      721 tcttgtcgat ggcctgctgg gcctcggccg ggccacggta gccggccggt tgaccaatct
      781 tgtccatttc agattgaaat tgcggattat cgacgacctg cttgcacacc tgaccccagt
      841 aattgatgat ctcgccgggg agcctctgcg ggccgccaag ggagcgatac cgctcggaga
      901 agacgtcgta gccgagctcc ttgaacgtcg ggacctcggg gagcttggaa tcgcgctgat
      961 cggtagcgat ggccaggggg cgcagctttc ccttctgtgc catggcatag agagaggtcc
     1021 ccaccatcac gtccgtggtg ccggcgaggg tcgcctggag cgcctgggcg ccgcccgtga
     1081 acgggatctg cgtgaacttg gttccggtcg cattttcgat gagcagcgtg gtcatgaaat
     1141 ccgctcccag cgcccccgtg ttcgccgcct tggtcttctc ggggttctgc ttggcgtact
     1201 cgacgacgtc cttcaacgtc ttgaactgac ccccctcccg ggcatagagc aggtcgggga
     1261 caatggcata aatgcagatg ttggtgaact ggtccatctt gtaacccgtc tgcttcggcc
     1321 ggaccagggc cggtatgatg ttgaacggcg gggtcgtgat ggtcaacgtg tacccgtccg
     1381 gcttggcgcg gaccatctcc gcccagcccg tggcgccgcc agccccgggc ttgttcacga
     1441 tgaccagccg ttgcccgatg agaggctccg cgtacttgtt gaacacccgc tgcgaggcgt
     1501 cggctccgcc gcccggatcg aagggcacga tgaactcaac cggccggctt ggatacccgg
     1561 ccggcttggc gatggcactc gactcgccgg cgaaagcagg cgcggcgccc aaaagcagcg
     1621 tggtcaacca caacgctacc agaaccctca gcaggatact tttcttcaga gtcatatcgg
     1681 ccccctttcc tgttgagcga accttccggc gacgttctcg cgcacgccat cagttgagtg
     1741 gacgtcccgt gtattccagg atgtacagcc caccccgcgc gcggtctgcc gcgtagatca
     1801 cgttgcgatc ctccacgaag acatcgctga tccggcaggc gcgctgtccc ggaggcgtct
     1861 cggggaggaa ggcgccaatc tcctccggcc ggaacgggtc gctgatgtcg tagatcctga
     1921 ggcccgcgct gaaccaggtg gcggccaccg tgtattgcag cctcgcactt cctggctccg
     1981 tctcgttctc gtggatgttg tgggccccga tgcggccgcc gatccggtgc agctcctcga
     2041 atcactccgg ggtggggaag ctggagatca tgaccggatt cgtttcctca cgtgcgtcca
     2101 ccatccagag ccgcttgggc cagtcgatgc cctcgtcccc cgtggcctcg tccgtcacga
     2161 ccaggaggtt ccgatcgaac agggggagca ccgtgtgcgt gaagccgggg aacggcggat
     2221 ggtaatccag ccggctgatc tggcgcggcc gtcccttgtc ggcaatgtcc aggatcacga
     2281 ttcccccgtc gatgtagccg aggtaggcgc ggtccgggcg ctcaggatag cactgggcgt
     2341 tgtggagacg aaaggcgaag tcgagggagg gcgccgtgtg gcgcggcggg agcgactcct
     2401 tgtcgccctt gcgctggccc ggcatccacc agcgtcccac ctccctgggc ttcgaggggt
     2461 tctccaggtc cacgatcatg tagaactggt gatctttcgg gtggctgggc tcgaaatcgg
     2521 gcgctcccgt ggagatgtgg gcgaagcgcc catccgtcag gaatacgaag tggaccccct
     2581 gggaatacgg gccggagacg tcgaagaatg cgacctccct gggcttgacc gggttggaga
     2641 cgtcgtagat ctggaagccg gccggctttt gcccgggcgt cttgcactgg taggccagga
     2701 ggagaaggtc gttgcgcagg gcgagggagt ttgcccgggc atcggcgtgg gggacaggaa
     2761 gttgccacag caatgtcggc ctggaggggt cggtcacgtc caggatggag aaggccatgg
     2821 gcggattctc gtgggcaatg tacaggatcc ggcgcccgtc ccgcgtgatc ttgaccccca
     2881 tgccttcgcc cgcgttggga gcgccgccca gatcggagtg gccgatgagt ttcagattgc
     2941 gtgcgattcg aaccgggggg acgttggtca tcttcgcctc ctgtgatgag agttgacatt
     3001 cccgccagcg gacgcccggc tccccagctc tgccctgtca gcggtcggtg agccggagca
     3061 gggcggcggc attgcgccgg aagatcttgt cggcgatctc gccggagaag cccatggccc
     3121 gatatgcctg caccgctccg ccgaccgggg cgaaggggta gccgctggcg aaaaggaacc
     3181 ggtcctgaag gtaggtccga gccgccaggg cgaagtcctc ccggccgggg agattgatgg
     3241 cgtagatatc gggcgacagg tagacgttgg tccgccggaa cgccacgccc accatcggct
     3301 ggacccaggg ccagcaggcg tgggcgatga ccagcgtgag ccccgggaaa tcgtgggcca
     3361 cctgatccac gtccacgggg agacagtagc tcacgtcggg accagtcatc aggctcaggg
     3421 tcaggatcac cgccagcccc cggtcggcgc agcgggcgta gatcgggtac aggcgacggt
     3481 cgttggcgcg catgggctgc ggggcaaacc caggctccat ggagatcgcc ggcatccgcc
     3541 agtctccggc gatccggtcc acctcccgaa gcgcggccgc cgtgtcgacc ggattgacgc
     3601 cgccgcacgc cacgaagcgg cccgggtatc cggcggcgat ggcgaccacc tcttcgttgg
     3661 tgaccgtgcc aaaccccgag ccggtgttgc ggccggtgat caccccgatc tccactcccg
     3721 cagcgtccat ctcggcgagc agcttttccg tggagagttc caggagcgag ggcggcggct
     3781 ccaggctgag ctgccgcgtt ctcgccatcg tccgctcttt ctcgcggaac atgatggtcg
     3841 tcagaaaccc cccggccggc ggccggagcc ggaaatcgat gatccccgtc atgcgatccc
     3901 tccctggggc ccgtccgtcc gctcgagctg ccgcaccagc ttgagtgccg cgatcaatag
     3961 cgcctccatc cgcgcacgcg cggcccgctc tcgctccgca tcccaggtga ggaatccccg
     4021 ccccgcgcgg gccccggtgt cgcctcgggc caccagtttt cggaggagcg gcgacggctc
     4081 gggcgcggcc tccagatccg ggatgatgta ctcgtggatc ttgaggatct gctccaagcc
     4141 gaccagatcc atggtttcca gcgtccccgc gcccaggagc cgaaatccga aaccccagcg
     4201 gaccgccgca tcgacatcct cggcgctggc gatgcctcgg gcgaccaggc tgatggcctc
     4261 tcgacgcatg gcctgctgga tgcgattgtt caggaagccg ggcacatccc gcatcaccgc
     4321 cacggggcgc ttgccgatcc ggcggagcag accacaggcc tgcgtcacga tctccggggc
     4381 cgtggcgtcc cccttgacca cctccaccag cggaaccagg tgcgccggat tcacgaactt
     4441 catgccgacc acccgctggg gcctccgggc gcgggccgcg atctgcgtga tgggcaggcc
     4501 cgaggtgttg ctggccagga tggtttcggg tcccacccgg ccctccagct ctgcgaccag
     4561 ctcctgcttc ccctcgagc
//
LOCUS       DBNGZC010000819         3046 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2125773, whole genome shotgun sequence.
ACCESSION   DBNGZC010000819 DBNGZC010000000
VERSION     DBNGZC010000819.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2125773"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /gene="waaF"
                     /locus_tag="AC16G0_14045"
     CDS             complement(<1..1105)
                     /gene="waaF"
                     /locus_tag="AC16G0_14045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HIR5100770.1"
                     /translation="MGADMTGGGGILEIDPQQVRRILVREVNWVGDGVLTLPALTALD
                     RRFPMAEISVLARSWVADLFTGQPAVDRVIQYQPEAYRGLLGRRRLAGEIRHQGFDLA
                     VLFPNSLDAALIPWIARVPCRVGYSTDGRRLFLTHPVRITAEGSGRHQVEHYLDIVRA
                     LGGDAGPVPRLHVTNDVRNATARFLKDNGISWSDLLVALNPGSVYGSAKRWPADRFAA
                     VADALVDGWGARIVLIGSEREVPILAEVARAMQRPGVMLGGRTDLTVLMGLLERAHLL
                     LSNDTGAMHVAAALDTPVVALFGPTDVQATGPLGCRSRVVREPVPCSPCLLRECPIDH
                     RCMTGVDVQQVLQAAGDLLETARGSRRVPFGSPE"
     gene            complement(1095..2066)
                     /locus_tag="AC16G0_14050"
     CDS             complement(1095..2066)
                     /locus_tag="AC16G0_14050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR5100771.1"
                     /translation="MAERSRWSPAVVAEWLLVAAVAKVFAWLPGSAAYAVGEGAGRLA
                     FRLDQRHRRITEENLARAFPGQYTPQALTALARSVFENLGRTAVDVARSDRLVHGPAQ
                     DILVMEGRDRILAARQRGKGVLFITAHFGPWELVPLIFALHAGWGNLVARPLDNPRLD
                     ELLTRLRERCGNRVIRKREAVHAVLQALRRGEMVGILIDQHISEREGVVVPFFGQPAS
                     TASAPALIAMRSGAAVFPGGVLRDGRGRYRLRVGEEVPVRRGGDLKADLVENTARFTA
                     AIEALIRERPDHWFWVHRRWKTSLPIDPRLMPKEGVRDQVGVARDGR"
     gene            complement(2059..>3046)
                     /gene="lpxK"
                     /locus_tag="AC16G0_14055"
     CDS             complement(2059..>3046)
                     /gene="lpxK"
                     /locus_tag="AC16G0_14055"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - lipid-A 4'-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HIR5100772.1"
                     /translation="NAGYSLGILPVHRLPCRVVCVGNLTVGGTGKTPTVVAVGERLLA
                     AGRKVCVLLRGYGRAGTAAEIVSDGRDLLLEWQRAGDEAILLGRLLPGVPVVVGGDRV
                     EAGNLAIRCFGPDTLLLDDGFQHRRLHRDVDLVLLDGTDPFGGDHLLPLGRLREPVGA
                     LRRAQAILVTRADQAGDLASLRRRLEECAPEVPVASAVHQPRRLVDLRSKRSMPLEAL
                     RGMKVLAVSGIAKPEGFLWTLRQLGATPAGVLAFPDHHQFTREDRARMGREAQTVGAE
                     CILTTEKDAVRLGQELPAGPPILALGVTIRLIQGEEIVGRVLGIAAGEVGRG"
BASE COUNT          534 a         1118 c          957 g          437 t
ORIGIN      
        1 tctctggcga gccgaagggc acgcgccggg agccgcgagc cgtctccagc aggtctccgg
       61 ctgcctggag gacttgctgc acgtcaaccc cggtcatgca ccgatggtcg atgggacact
      121 cgcgaaggag acagggactg cacggcaccg gctcccgcac cacacggcta cgacacccca
      181 ggggacccgt ggcttgcacg tcggtgggcc cgaagagggc caccacaggc gtatccaggg
      241 cggctgcgac atgcatcgcc cccgtgtcgt tgctgaggag cagatgggct cgctccaaga
      301 gacccatcaa caccgtgaga tcggtcctgc ctcccagcat gacccccggc cgctgcatcg
      361 ctctcgcgac ctctgccagg ataggaacct cacgctccga gccgatgagg acgatccgtg
      421 cgccccagcc gtccaccagc gcgtcggcca cggccgcaaa ccggtcggcg ggccagcgct
      481 tggcgctccc atacaccgag ccgggattca acgccaccag gaggtcagac cagcttatcc
      541 cattatcctt taagaatcga gcggttgcat tccgaacgtc attcgtgaca tgaagtcttg
      601 ggacaggccc tgcgtcccct ccaagcgccc ggacgatgtc taagtagtgc tctacctgat
      661 gccgtccaga tccttcggct gtgatccgga ccgggtgggt gaggaagagg cggcggccgt
      721 cggttgagta ccccacccgg caaggaacgc gcgcgatcca tggaatgagc gccgcatcca
      781 gagaattcgg aaataacacc gcgagatcga acccctggtg ccggatctct ccggccaagc
      841 gccgcctccc gagcagaccc cgataggcct ccggctgata ctggatgacc cgatccacgg
      901 caggctgccc ggtgaagaga tctgccaccc acgatctcgc caggacgctg atctccgcca
      961 tggggaagcg gcgatccagc gccgtgagtg ccggcaaggt aaggactccg tccccgaccc
     1021 agttcacttc ccggacgagg attcttcgga cctgctgggg atcgatctcg aggatgcctc
     1081 caccacctgt catgtcagcg cccatcccgg gcaacaccaa cctggtcccg gactccctct
     1141 ttcggcatca gccgtgggtc gatcgggagg gaggtcttcc agcgccggtg cacccagaac
     1201 cagtgatccg gccgctcgcg gatgagcgcc tcgatggcgg ccgtgaatcg cgctgtgttc
     1261 tcgaccaggt ccgccttgag atctccgccc cgtcgcacgg gaacctcctc gccgacccgg
     1321 agccggtacc gcccccgccc atcgcgcagg accccccccg gaaagacggc cgcccccgac
     1381 cgcatggcga tcagcgccgg agcggaggcc gtcgaggccg gttgaccgaa aaatggaacc
     1441 accacgccct ctcgctcgct gatgtgctgg tcgatgagaa tgcccaccat ctccccacgg
     1501 cgcagtgcct gcaggaccgc atgcacggcc tcgcgcttcc ggatgacccg attgccgcat
     1561 cgctcccgga gcctcgtgag caactcgtcc agccgcggat tgtccagagg ccgcgccacg
     1621 aggttgcccc agccggcgtg cagggcaaag atcagcggca ccagctccca cggcccgaag
     1681 tgggccgtga tgaagagcac acccttgccg cgctgccgcg ctgccaggat ccggtcgcga
     1741 ccttccatga caagaatatc ctgggccggc ccgtgcacca gacgatctga tcgcgccacg
     1801 tccacggccg tccggcccag gttttcgaac accgatcggg ccaaggccgt caatgcctga
     1861 ggggtgtact ggcccgggaa ggcccgcgcg aggttctcct cggtgatgcg acggtgtcgc
     1921 tggtccagtc ggaatgccag cctcccggcc ccctcgccca cggcgtaggc ggcggatccc
     1981 ggcagccagg cgaagacctt ggcgacggcg gccacgagca gccactcggc cacaaccgcc
     2041 ggactccacc gagagcgttc agccacggcc gacctccccc gccgcgatcc cgagcacccg
     2101 tcccacgatc tcctccccct ggatgagcct gatggtaacg cccagggcca ggatcggcgg
     2161 gcccgcaggc agctcctgcc ccagccgaac ggcgtccttc tccgtcgtga ggatgcactc
     2221 ggctccgacc gtctgcgcct cacgccccat ccgcgcccga tcttcccgcg tgaattggtg
     2281 atgatcggga aaggctagga ctccggccgg cgtggcgccc aattgtcgga gcgtccagag
     2341 aaacccctcc ggcttggcga tcccgctcac cgccagaacc ttcattcccc ggagcgcttc
     2401 cagcggcatt gagcgtttcg agcgaagatc cacgagccga cggggctggt ggaccgcgga
     2461 tgccaccggc acttcgggag cgcactcttc caggcggcgc cgcagcgagg caaggtcgcc
     2521 ggcctgatcg gcgcgggtca ccaggatcgc ttgggcgcga cggagggcgc ctacgggttc
     2581 cctcaggcgt cccaggggca ggaggtggtc accgccgaag gggtccgtcc catccagcag
     2641 gaccaggtcc acgtcccggt gcaaccgccg atgttgaaag ccatcgtcca ggaggagggt
     2701 gtcgggaccg aagcagcgga tggccaaatt cccggcctcc acccgatccc cgccgaccac
     2761 gacggggacc ccaggaagca ggcgcccgag caggatggcc tcatccccgg cgcgctgcca
     2821 ctccaggagc aggtcccgtc cgtcggaaac gatctccgcg gccgtcccgg cgcgcccgta
     2881 gccgcgaagg aggacgcaga ccttgcgccc ggcagccagc aggcgttcgc ccacggcaac
     2941 caccgtggga gtcttcccgg ttccgcccac ggtcaggttc cccacgcaca cgacccgaca
     3001 cggcaggcgg tggaccggca ggatgcccag gctatagccg gcgttc
//
LOCUS       DBNGZC010000820         7165 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_656959, whole genome shotgun sequence.
ACCESSION   DBNGZC010000820 DBNGZC010000000
VERSION     DBNGZC010000820.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7165)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7165)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7165
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_656959"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..119)
                     /locus_tag="AC16G0_14060"
     CDS             complement(<1..119)
                     /locus_tag="AC16G0_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR5100773.1"
                     /translation="MSGHSKWASIKHKKGKVDAQRGRVFTKLIREITVAARVGG"
     gene            397..1002
                     /locus_tag="AC16G0_14065"
     CDS             397..1002
                     /locus_tag="AC16G0_14065"
                     /inference="COORDINATES: protein motif:HMM:NF016216.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HIR5100774.1"
                     /translation="MGKARPNFFELLKTRPVIAGLRQSGSVETAIRRGVGVLFILGED
                     IFALKDSVAKAHAEDRLILAHVDLIKGIGRDEAGVRFLARDLGVDGILTTRANLIGPA
                     RREGLIAVQRLFVLDSESLEAGLPTVQRAGPDAVEVLPGVILPLIAIRLKEGSLPPLI
                     AGGLIRTPRQVEEILQAGAVGISTGEESLWGYRRGEERKPR"
     gene            999..2648
                     /gene="glpD"
                     /locus_tag="AC16G0_14070"
     CDS             999..2648
                     /gene="glpD"
                     /locus_tag="AC16G0_14070"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HIR5100775.1"
                     /translation="MIGRLEALNGQAFDLAVIGGGMAGAGIARDAALRGYRTLLLERK
                     DYAYGTTSRSSKLIHGGLRYLELFDVGLVRESLTERERLQRLAPHLVRPLPFLIPVYR
                     GAKRGMFMIRLGMKFYDLLTPGKRTEHYRTLSRQETLRREGYLEPQDLLGAGYYFDDL
                     LLFPERLCLENVLSARRWGASVVNYAEVAGIKRRARSAERRAGNSDWDIEVRDVIGGG
                     AARVTARVVVNASGPWVDDLRRRAGVDGGRRTLRTTKGIHLLLPRMTDHAVYIAAKQD
                     ERMFFVIPWREFSLIGTTDTDFTGDLDRLAATREEVEYLLKETKRVFPAAHVREDQIA
                     YTYAGVRPLTLKEGAAASAVSRQHKVIAEGDPRTFLSITGTKLTCYRSLAEEAVDQVG
                     RLLGRPAPCRTHRLALDGSDGEDTLEVRLWADVSDLARQTGLEPAQIQSLMETYGRRY
                     PAVLEVAARSPELRGRLCKQNPDIRAQLVYALEHEAVETLCDFMLRRTGIGTSACLGK
                     DCCEQIGVWMGEARGWDRRRIDREIGEYLDEIALGQQFRNQ"
     gene            2754..3722
                     /locus_tag="AC16G0_14075"
     CDS             2754..3722
                     /locus_tag="AC16G0_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5100776.1"
                     /translation="MKNSLTSDSTAVVAPGDLAAHGHAVATTWRIWLRGGGTYLYLLP
                     TLVFLALFTYYPIAFSAYLSLFRWNVLNPERIFVGLQNYLALWQEPLFWLVLRNNLFY
                     ALGTIPTTMGLALFLAVLTNQKLGRVRSLYRVALFYPTMIPMVAAAMLWVWIFNQGIG
                     LLNYYASFLGVPHIEWLYDRHWALPAIMIMSLWKNFGYFMLIYLAALQGVSADLYEAA
                     SIEGAGAWRRFWGITFPLLTPASLFVFVVAIISSFQVFDQVFVMTQGGPADQTNVLTF
                     FIYQNAFRFWDIGMGATLTTIFICLLLILIVVVFRSVGRHVYYEVE"
     gene            3737..4594
                     /locus_tag="AC16G0_14080"
     CDS             3737..4594
                     /locus_tag="AC16G0_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018527837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5100777.1"
                     /translation="MTVGERVRVWNRRFRLHRLATHLTLSLVTLVTVTPYLWLLSTSL
                     KLRTEVFTPTPKWLPWPVNFANYAEVFDRAPFGIFLINTIIVVTGILAVQLLTVTLAA
                     YVFARRKFRGADVLFLLFLIQMMIPIHATFLPNFLTLRNLDLLNTRVAMMLPFFASGY
                     GTFLLRQAFKQIPRDLEDAAVIDGCGGLRFLRHVLLPLAKPTLIAFALISLVSHWNDY
                     LWPLIVTDTPDVRTLTIGLGMFVQQESGADWTLLMAATAFVTAPLMVIFLVFQRRFIE
                     SFMFSGLKG"
     gene            4714..5994
                     /locus_tag="AC16G0_14085"
     CDS             4714..5994
                     /locus_tag="AC16G0_14085"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5100778.1"
                     /translation="MRRGWMVGLLVLVLAVAFAVGTADAASKLIRLTFYYPVGVAGPL
                     AKVIGDMTDKFNKDNPGIEVVPVYSGDYDPTLQKVQTSVMAGNPPDIFIVEISELPTL
                     LAMNAIIPLDDYVKKAEGGKYWDDFFPAFRENSMLRGKIWWIPFQRSTPVFYWNKELF
                     KKAGLDPERPPQTWTELKEVAKKLTVREGNEVKQWGVTISGGWNDWEFEGFVRQNGSW
                     LINPEGTKVNFDTKEAVEALEYWVDLMYKEKVGPPHSTWGSTPPDFVAQRTAMLYHST
                     GILTFLRNSAKFDFGVAFMPKNKTYGAEVGGGNLAISRKIPKERQDAAWKFIEWMTTS
                     ENAAQWSLASGYIATRKSAYDIPAMKEFVGKDPRYLVARDQLQFAAGKMMAPNFQKIR
                     EILKKQLDDAVDGKVTPKEAMVTVQKQVEAVIKR"
     gene            6240..7034
                     /locus_tag="AC16G0_14090"
     CDS             6240..7034
                     /locus_tag="AC16G0_14090"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR5100779.1"
                     /translation="MRFLAVQGHRGASALAPENTLAAFRKAMEIGADGMEMDLQLTRD
                     GAVVVIHDDTLERTTDGRGRVTDLTLAEVRRADAGVKFGSAFRGERIPTLTEVIDLVK
                     AGGNDRVRLNLEIKFGEGREGTPEEIEAHVLAILRATGFVERVTVQSFHHPSPAKVKG
                     LEPRIPTGLLVEARQPPPDPVALVRQYRADYYAPEHRLVTPELVRTLHQAGIPVVTWT
                     VNEEPIMRRLIDAGLGSMRGDAIISDYPDRAVNLRRTLSEPKKEGT"
BASE COUNT         1268 a         2252 c         2356 g         1289 t
ORIGIN      
        1 ccccccacgc gggcggctac cgtgatttcc cgaatgagct tggtgaagac ccggccgcgc
       61 tgggcgtcca ccttgccctt cttgtgcttg atgctggccc acttgctgtg tccggacatg
      121 gcagcacccc ctgatcgagg agagccgaaa gttaccatag cggcgcgaag gttgccaagc
      181 gaaaactccg gtaagggaag gatcgcggac catccgggct caacacacat accggcgatt
      241 cgccttgaca ggcagggaag gcgagaatag agtggcggca aagcgggaag gggtagatcg
      301 aggtcctcgc cggggcggag agttgagaaa gtcctaacgg gggcgatccg aggggatcgt
      361 ctttctgtta ggatttttta tttccgggag gagtggatgg gaaaggctcg gcccaacttc
      421 ttcgagctgc tgaagacccg cccggtgatc gcggggctgc gacagtcggg gagcgtcgag
      481 accgcgatcc ggcgcggggt gggcgtcttg ttcatcctgg gcgaagacat cttcgccctg
      541 aaggacagcg tcgccaaggc tcatgcggaa gaccggctca tcctggccca cgtcgacttg
      601 atcaagggta tcggtcgtga cgaagcaggg gtgcgcttct tggcccgcga cctgggggtg
      661 gacggcatcc tcaccacacg ggcgaatctg atcggccccg ccaggcggga ggggttgatc
      721 gccgtccagc gtctattcgt cctggattcg gaatcactgg aagcggggct ccccaccgtc
      781 cagcgagcgg gccccgacgc ggtcgaggtg ctgccggggg tgatcctgcc actgatcgcc
      841 atccgcctga aggagggatc gttgccgccc ctgatcgccg gcgggctcat ccggacgccg
      901 cggcaggtgg aagagatcct gcaggccggg gcggtgggca tttccaccgg cgaggagagc
      961 ctgtggggtt atcgacgagg cgaggagcga aagccacgat gataggtcga ctggaagcct
     1021 tgaacgggca ggcgttcgat ttggcggtca tcggcggcgg catggcgggg gcggggattg
     1081 cgcgagatgc cgcgctacgc ggctaccgga ccctgctgct ggagcggaag gattacgcct
     1141 acggcacgac cagccggtct tcgaagctga ttcatggggg cctccgatac ctggaactct
     1201 tcgacgtcgg actcgtgcgc gagtccctca cggagcggga gcggctgcag cgccttgctc
     1261 cccacctggt ccgccccctg ccgttcctga taccggtgta ccggggcgcc aaacgcggca
     1321 tgttcatgat ccggctgggg atgaagttct acgatctcct cacgccggga aagcgaaccg
     1381 agcactaccg gaccctgtcc cgacaagaga ccctgcgccg cgaggggtac ctcgagcccc
     1441 aagacctcct gggggccgga tactacttcg acgacctcct gctgttcccg gagcggttgt
     1501 gcctggagaa cgttctctcg gcacgccgct ggggggcatc ggtcgtgaac tatgcggagg
     1561 tcgcgggcat aaagcgccga gcgcggagcg ccgagcgccg agctggaaac tccgattggg
     1621 acatcgaggt ccgagatgtg atcggtggcg gggcggcccg cgtgacggcc cgggtggtgg
     1681 tgaatgcctc cggtccatgg gtggatgacc tccgccgccg tgccggcgtg gacgggggac
     1741 ggcggaccct ccgaacgacg aaggggattc atctcctgct cccacggatg accgatcacg
     1801 cggtctatat cgcggccaag caggacgagc gaatgttctt cgtcatcccc tggagggagt
     1861 tcagcctgat cggcacgacg gacacggact tcacggggga cctggaccgc ctggccgcca
     1921 ctcgcgagga ggtggagtac ctcctcaagg agaccaagcg ggtattcccc gccgctcacg
     1981 tgcgcgaaga tcagatcgcc tacacctacg ccggcgtgcg tccgctcacc ttgaaggaag
     2041 gggcggcggc gtccgcagtc tcccggcagc acaaggtcat cgccgagggc gacccccgca
     2101 cgttcctttc catcaccggg acaaaactca cctgctaccg gagcctggcg gaagaggccg
     2161 tggaccaagt cgggcgcctc ctgggccgcc ccgccccgtg ccgaacccac cgcctcgcgc
     2221 tggacggctc cgacggcgaa gacaccctgg aggtgcgcct ctgggcggac gtcagtgatc
     2281 tcgcccgaca gaccggcctg gagccggcgc aaatccagag cctgatggag acctacggac
     2341 gccggtaccc ggccgtgctg gaggtcgcag cccgatcccc ggaactgcgg gggcgcctct
     2401 gcaagcagaa ccccgacatc cgggcccaac tcgtctatgc cctggagcac gaggcggtcg
     2461 agaccctgtg cgacttcatg cttcgccgga ccggtatcgg aacaagcgcc tgtttgggca
     2521 aggactgttg cgagcagatt ggcgtctgga tgggggaggc ccgggggtgg gaccgccggc
     2581 gcatcgatcg ggagatcggg gagtacctgg acgaaatcgc gctgggacag cagtttcgga
     2641 accagtagca ggctgaaaac gttcggctgc aagaggggtt cggctgtctc ctccccgtca
     2701 cctggagcgg agcgggggcg gccgtgccgc cgaaggcttt ccgggactct gacgtgaaaa
     2761 acagcctgac ctccgattcc accgccgtgg tcgctcccgg agacctggcc gcgcacgggc
     2821 atgccgtggc aacgacgtgg cggatctggc tccgcggggg cgggacctac ctctacctgc
     2881 tccccaccct cgtctttctg gcgctcttca cgtactatcc catcgccttc tccgcctacc
     2941 tcagcttatt ccgctggaac gtgctgaacc cagagcgaat attcgtcggc ctgcagaatt
     3001 acctggcgct ctggcaggag ccgctcttct ggctcgtcct gcgcaacaat ttgttctatg
     3061 cgctgggcac gatccccaca accatgggtc tggccctttt cctggcggtc ctgaccaacc
     3121 aaaagttggg ccgcgtacgg agcctctacc gggtagccct gttctatccc accatgatac
     3181 ccatggtcgc cgccgccatg ctctgggtct ggatcttcaa tcaagggatt ggcctcttaa
     3241 actactacgc cagcttcctg ggagttccgc acatcgagtg gctgtacgat cgacactggg
     3301 ccctgccggc catcatgatc atgagcctct ggaagaactt cggctacttc atgctgatct
     3361 acctggccgc gctccagggg gtttccgccg acctgtacga ggcggccagc atcgaggggg
     3421 ccggggcctg gcgccgcttc tggggcatca cctttcccct gctcaccccg gcgagcctgt
     3481 ttgtgttcgt ggtggccatc atctcctcgt tccaggtctt cgatcaggtt ttcgtcatga
     3541 cgcagggcgg gccggcggac cagacgaacg tcctcacctt cttcatctat cagaatgcct
     3601 tccgcttctg ggacatcggc atgggagcca ccctgacgac catcttcatc tgcctgctcc
     3661 tcatcttgat cgtggtggtg tttcgatcgg tcgggcgcca cgtctattac gaggtcgagt
     3721 aatcccgggg gtctgcatga cggtcggcga gcgggtccgg gtctggaatc gacgcttcag
     3781 gctgcatcgt ctggccacgc acctgacctt gagcctcgtc accctggtga ccgtgacgcc
     3841 gtacctgtgg ctcctctcca cgtcgctcaa gctccggacc gaggtgttca cgccaacacc
     3901 caagtggctc ccctggcccg tcaacttcgc caactacgcc gaagtcttcg accgggcccc
     3961 cttcggcatc ttcctgatca acaccatcat cgtggtgacc ggcatattgg ccgtgcagct
     4021 cctgacggtc accctggcgg cctacgtctt cgcgcggcga aaattccggg gagccgatgt
     4081 cctcttcctg ctcttcctca tccagatgat gatcccgatc catgcgacgt tcttgccgaa
     4141 cttccttacg ctccgcaacc tagacctcct gaacacccga gtcgccatga tgcttccctt
     4201 cttcgccagc ggctacggga cattcctgct gcgccaggcg ttcaagcaga tcccgcggga
     4261 cctggaagat gcggcggtca tcgacggctg cggcggcctc cgattcctgc ggcacgtcct
     4321 gctgcccctg gcgaagccga ccctgatcgc ctttgccctg atcagcctgg tgagtcactg
     4381 gaacgactac ctctggccgc tgatcgtgac cgacacgccg gatgtgcgga cgctcaccat
     4441 cggtctgggc atgtttgtcc agcaggagag cggcgccgac tggacactcc tcatggcggc
     4501 gacggccttc gtcacggcgc cgctcatggt catcttcctg gtcttccagc ggaggttcat
     4561 cgagagcttc atgttttcag ggctgaaggg ctagggcaag aatgacttat cccccatgac
     4621 caaatcccaa cggggaaggc gagcacatgc gtcggccgct ggtcatcgag attggtcatt
     4681 tgtctctgcc tgaggcgaga gaggaggcgc gtcatgcgac gaggatggat ggtgggactg
     4741 ctggtcctgg tgctggccgt ggccttcgcc gtcgggacgg cggacgcggc cagcaagctg
     4801 atccggctca ccttctatta tccggtgggg gtggccggcc ccctggccaa ggtcatcggg
     4861 gacatgaccg acaagttcaa caaggacaac ccggggatcg aggtcgtccc ggtctactcg
     4921 ggcgactatg accccacgct gcagaaggtc cagacgtccg tcatggccgg aaacccaccc
     4981 gatatcttca tcgtggagat ctcggagttg ccgacgctct tggccatgaa cgccatcatc
     5041 cccctggacg actacgtcaa gaaggcggag ggggggaagt actgggatga cttcttcccc
     5101 gccttccgcg aaaacagcat gctcaggggg aaaatctggt ggatcccgtt ccagcggagc
     5161 acgccggtct tctactggaa caaggagctg ttcaagaaag caggcttaga ccccgagcgg
     5221 cccccgcaga cctggaccga gctaaaggag gtggccaaga aactcacggt ccgggagggg
     5281 aacgaggtga agcagtgggg cgtgaccatc tcgggcggct ggaacgattg ggagttcgag
     5341 ggcttcgtcc gtcagaacgg gagctggctg atcaatcccg aggggaccaa ggtcaacttt
     5401 gacaccaaag aggcggtgga ggccctggag tactgggtgg acctgatgta caaggaaaaa
     5461 gtggggccgc cgcattccac gtggggttcc acgccgcccg acttcgtggc ccagcggacg
     5521 gccatgctct accactccac ggggatcctg accttcctgc ggaactcggc aaagtttgac
     5581 ttcggggtgg ccttcatgcc caagaacaag acctacggcg ccgaggtggg aggcgggaac
     5641 ctggccatct cccgcaagat cccgaaggag cggcaggacg cggcctggaa gttcatcgag
     5701 tggatgacca ccagcgagaa tgccgcacag tggagcctcg cgtcaggcta catcgccacc
     5761 cggaagtccg cctacgatat cccggccatg aaggagttcg tcggcaagga tccccgctac
     5821 ctcgtggcgc gcgaccagct ccaattcgcg gcgggcaaga tgatggcccc caacttccag
     5881 aagatccggg agatcctcaa gaagcagctg gacgatgccg tggacggcaa ggtgaccccg
     5941 aaggaagcca tggtcacggt gcaaaagcag gtcgaggcgg tgatcaagcg gtgacgcgtt
     6001 ttccccggcg agaggcccgc gcccttgcga tgtgctctct gcaagggcca gggccttcgc
     6061 cgcgaacgga gcagcgcggg gaatggtgta gccaatattc cgcgatgcgt agtatagtcc
     6121 ccggcgcagg gctctcccgc cttcgggagc ctgcgtgtct tctttgagcg cactgggttg
     6181 tccggcggct gcgcacggtg cccggcagcg ggctggccgc gtctgcgaaa ggagatagcg
     6241 tgagattcct ggcagtgcag ggacatcggg gggcttccgc cctggcgccg gagaataccc
     6301 tggccgcctt ccggaaggcc atggagattg gcgcggacgg catggagatg gacctgcagc
     6361 tcacgcggga cggggcggtg gtggtgattc acgacgacac gctggagcgc accactgacg
     6421 gtcggggcag ggtcaccgat ctcaccctgg ccgaggtgag gcgggcggac gccggggtga
     6481 agtttggatc tgccttccgc ggggaacgca tccccaccct caccgaggtc atcgacctgg
     6541 tcaaggccgg cgggaacgac cgggtgcgac tgaacctgga gatcaagttc ggtgaagggc
     6601 gggaaggcac acctgaggag atcgaggcgc atgtcctggc cattctgcgt gccaccggat
     6661 tcgtcgagcg cgtgaccgtt caatccttcc accacccgtc cccggccaag gtgaaggggc
     6721 tggaaccccg catccctacc ggtctcctgg tggaggcgcg ccagcctccc ccggacccgg
     6781 tggccctggt tcggcagtac cgggcggatt actatgcgcc cgaacaccgc ctggtcacgc
     6841 cggagctggt gcggacgctc caccaggccg ggattcccgt ggtgacctgg accgttaacg
     6901 aggagcccat tatgcgtcgt ctgatcgacg cggggttggg ctccatgcgg ggagacgcga
     6961 tcatctcgga ctatccggat cgtgcagtga acttgcggag aaccttgtcc gaacccaaga
     7021 aggaggggac atagcaaagg catgtgttgc tttcgtcggc cactcaattt gctcgtcagt
     7081 ctggggatgg taggtatcgc cgcttccctt atcggcggtg cgtctttcct gaagggcgcg
     7141 gccgccggag aggcaccgcc gagtc
//
LOCUS       DBNGZC010000821         2208 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1441153, whole genome shotgun sequence.
ACCESSION   DBNGZC010000821 DBNGZC010000000
VERSION     DBNGZC010000821.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1441153"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="AC16G0_14095"
     CDS             <1..291
                     /locus_tag="AC16G0_14095"
                     /inference="COORDINATES: protein motif:HMM:NF048699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7305 domain-containing protein"
                     /protein_id="HIR5100780.1"
                     /translation="NPTNPTAPGAQTCTDVSIVGNGEFYGAVYAPNADINVSGNGAVF
                     GALAGKTVRINGNGGFHYDEALGTLGENVQTINGANAVTHRYSRYLWREIPF"
     gene            complement(366..1103)
                     /locus_tag="AC16G0_14100"
     CDS             complement(366..1103)
                     /locus_tag="AC16G0_14100"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HIR5100781.1"
                     /translation="MRPRIGITSWHRNDGDKLERWEAIRDTYTGAVTKAGGLPIILPI
                     ADDAPDLIGDFLNTLDGLLFTGGEDVAPAYYGEAIDERCQEPDGERDLFEIHLARAAL
                     ARQTPVLGICRGLQLLNVAAGGTLYQDIACRPGTLPQHSASAPDRQKLVHAVRILPGT
                     RLHSIMGVAESRVTSTHHQFVKDLAPGFRVTAESLEDWIVEAVEHPGYPFLLAVQWHP
                     ERMVHEHAAHLALFRGLVEAAGRARIE"
     gene            complement(1163..>2208)
                     /locus_tag="AC16G0_14105"
     CDS             complement(1163..>2208)
                     /locus_tag="AC16G0_14105"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR5100782.1"
                     /translation="ACFSLPDELATLTAAAGRMGGILTTHMRSEGDDLLESIQEVISA
                     AETARTPLQISHLKTYGERNWEKLPQAFELIESAQRRGLDVTADRYPYTAANTELQAV
                     LPRWAVEGSKAEQTARLKDPAVRERIRQEIDEGPESRNWSQVVISDVTLPQNRRYQGL
                     QVDAAARLAGKGTLDFVLDLLHEEKTQVDAIYFVMREDNLRMILRKPYVMIGSDSGCR
                     AHYGPLAKGRPHPRTFGSFVRVLGHFTREERLLDLPTAIRKMTSDPCRKLGLEDRGFL
                     RPGCKADVVLFDPDRVRDTATYEEPIRYPVGVHMVLVNGVVAVEAGKHTGARAGQILG
                     RTATKSVTGPGLA"
BASE COUNT          394 a          731 c          675 g          408 t
ORIGIN      
        1 aaccccacca accccaccgc gcctggcgcc cagacctgca ccgacgtcag cattgtcggt
       61 aatggcgaat tttacggcgc cgtgtacgct ccgaatgcgg acatcaacgt atccggcaat
      121 ggcgcggtgt tcggcgcact cgccggcaag acagtgagaa tcaacggtaa cggcggattc
      181 cattacgacg aggcccttgg gacactgggc gagaacgtgc agacaatcaa tggggcgaac
      241 gcggtgacac accgctattc ccgttacctg tggcgggaga ttccgttcta gggcgtagag
      301 cagaacacag caaagggcgg ggaggcggca ggaggccttc ccgccctttg cttttctcct
      361 gcggtctact ctattcgcgc tcgccccgcc gcctccacca atcctcgaaa cagcgccagg
      421 tgcgccgcgt gttcatgaac catccgctcg ggatgccact gaacggccaa caggaaggga
      481 taccctggat gctcgactgc ctcgacgatc caatcctcga ggctctcggc ggtgacgcgg
      541 aatccggggg cgagatcttt cacgaactgg tggtgggtgc tggtgactcg agactcggcc
      601 acacccatga tcgagtgcag cctcgttccc ggcaggatcc gaacggcgtg gacgagcttc
      661 tgtcggtcgg gggcactggc cgaatgctgc ggcagagttc ctggccggca cgcgatatcc
      721 tggtacaagg tgcccccggc ggccacgttg agcagttgga ggccccggca gatacccagc
      781 accggcgtct gccgcgccag ggccgctcgc gcgagatgga tctcgaagag atcgcgttcc
      841 ccatccggct cctggcagcg ctcgtcaatt gcctcgccgt agtaagccgg cgccacgtcc
      901 tcccctcccg tgaagaggag tccgtccagc gtgttgagga agtcgccgat caggtcgggg
      961 gcatcgtcgg caatcggcag gatgatcggc agcccacccg ccttggtgac agcgccggtg
     1021 tacgtatctc ggatcgcctc ccagcgctcc agcttgtccc cgtcattccg atgccaggat
     1081 gtaatcccaa tcctcggtcg cacgtcttct tcctttcttt cgtgaagccg attgaaattt
     1141 gcgtattctg caaaatcctc agctaagcca agcctggtcc cgtcacgctc ttcgtcgcgg
     1201 tccgtcccag gatctgcccc gcccgggccc cggtatgctt tccggcctcc accgccacga
     1261 ccccattcac caggaccatg tgcacgccga ccggatatcg gatgggctcc tcgtacgtcg
     1321 cagtgtcccg cacccgatcc ggatcgaaga ggaccacgtc cgccttgcag ccggggcgga
     1381 ggaaaccgcg atcctcgaga cccagcttcc ggcaaggatc ggaggtcatc ttccggatgg
     1441 cggtgggcag gtcgagcagg cgttcctccc gtgtgaagtg acccaggacc cggacaaacg
     1501 atccaaaggt ccgcggatgt ggacggccct tggcaagggg tccgtaatgg gcgcggcagc
     1561 ccgaatcgga accgatcatc acatacggtt tcctcaggat cattcgcaga ttgtcctcgc
     1621 gcatcacgaa gtagatggca tccacctggg tcttttcctc gtggaggagg tcgagcacga
     1681 aatccagcgt gcccttgccc gccaggcggg ccgcggcatc cacctggagg ccctggtacc
     1741 gccgattctg ggggagcgtg acatccgaga tcaccacctg gctccagttg cgggactccg
     1801 ggccctcgtc gatctcctgt cggatgcgct cgcgtacggc gggatctttc aagcgtgcgg
     1861 tctgctccgc cttgctcccc tcaacggccc agcgcgggag gaccgcctgg agttccgtgt
     1921 tcgccgccgt ataggggtag cgatcggcgg tcacatccag gccacgccgc tgcgctgact
     1981 cgatcaactc gaaggcctgg ggaagcttct cccaattgcg ctcaccgtat gtcttgagat
     2041 gggagatttg gaggggcgtc cgtgccgtct cggccgcgga aatgacctct tgaatcgact
     2101 ccagcaagtc atccccctcg ctccgcatgt gcgtcgtgag aatcccgccc atcctccccg
     2161 cagcggcggt gagggttgcc agctcgtccg gcaaggaaaa acaggcag
//
LOCUS       DBNGZC010000822         2006 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1933587, whole genome shotgun sequence.
ACCESSION   DBNGZC010000822 DBNGZC010000000
VERSION     DBNGZC010000822.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1933587"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1053)
                     /locus_tag="AC16G0_14110"
     CDS             complement(157..1053)
                     /locus_tag="AC16G0_14110"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5100783.1"
                     /translation="MTGARSSSGMIQDYRTAGGERQAKPTESRPPRPPGLASADSRQF
                     FFLRRLKAFAIVQMVEFDRLVGRDRVSDLTAVGIMTPSPSKPSRATRRRAQSFESLVQ
                     AGLTVIAKHGLYETTVQHITEAADVGKGTFYAHFPSKDDLVHHLVRHGFDEMIAAGHA
                     VPPVGGTPAERLANLIRAQHRVLGRRRDLIILLHQVRGLLILRPEARQRLRREYQRYI
                     EFLAEECRRVLGPRSLSRGEAQDLACAVAGFVSGTLSFEMLVHGVRGGRVAPNGPINA
                     FAAGVAARYVPSRRPGNGGQPR"
     gene            1052..1684
                     /locus_tag="AC16G0_14115"
     CDS             1052..1684
                     /locus_tag="AC16G0_14115"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR5100784.1"
                     /translation="MGLIMERVELETPQPIECDADHPLVSRAQAGDVAAFEALVRRYE
                     RWVFTLALRMLGDRGEAEDMAQEVFLKTYRGLKGFKGTSRFSTWLYAIASHHCLNYLR
                     ARRRRPEASGGWAEGEDGEELQAADRLPDGAPRADALLERADLARIVQTELTYLTEDH
                     RLILILRDIQGLTYGEIAEALGVELGTVRSRLHRARAEMRERLAVHLQTG"
     gene            1688..>2006
                     /locus_tag="AC16G0_14120"
     CDS             1688..>2006
                     /locus_tag="AC16G0_14120"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR5100785.1"
                     /translation="MICDEVREAFSEFYDDMLSGTRLTDLTQHLDECSECRAEWEAFS
                     TTMQVVTRLGMADPSPGFAARVRQQIEAPPWWGRLGHSLFVPLHVKVPIHAVALALLS
                     FAGL"
BASE COUNT          339 a          650 c          678 g          339 t
ORIGIN      
        1 gtgtgaagga tagctccttc ccgaatcgtg cgccagcata tgcgttgccg cccctcagtt
       61 caagaaaaaa agtcacggtg gccgcaccgg cgggcgtgag ccagccgcca cacctcccgt
      121 tgtgtatggg catcctcctt gggagcgccc ggcccctcag cggggttgcc ccccattccc
      181 gggacgccgc gacggcacgt aacgcgcggc gacgcccgcg gcaaaggcgt tgatggggcc
      241 gtttggggcg acccgtcccc ctcgaacccc gtggaccagc atctcgaagg agagagtccc
      301 cgagacgaat ccggcaacgg cgcaggccag atcctgagcc tcgccccggc tgaggctgcg
      361 cggtcccagc acgcgccggc attcctccgc gagaaactcg atgtagcgct ggtattcccg
      421 tcgcaggcgc tggcgcgcct ccggtcgaag gatgagaaga ccccgcacct ggtgcagcag
      481 gatgatcaga tcccggcggc gtcccagcac ccgatgctgg gcccggatca agttcgccag
      541 ccgctccgcc ggcgtgccac cgacgggcgg cacggcgtgc cccgcggcga tcatctcgtc
      601 gaagccgtgc cggacgaggt ggtggaccag atcgtccttg gaggggaagt gagcgtagaa
      661 ggttcccttg ccgacgtcgg cagcctcggt gatatgctgc acggtggtct cgtacaggcc
      721 gtgctttgcg atgaccgtca gtccggcctg gaccagactt tcgaagctct gggctcgccg
      781 ccgcgtggcg cgactcggtt tggagggcga aggcgtcatg atccccacag cggtgagatc
      841 ggatactcgg tcacgtccga ctaatcggtc aaattcgacc atctggacga tagcaaaggc
      901 cttcagcctt cgcaagaaaa aaaattggcg ggagtcggcc gaggccaggc caggcggacg
      961 gggtggacgg ctctccgtcg gcttggcttg acgctcgccc ccggcggtgc gatagtcctg
     1021 gatcattccc gaggaacttc ttgcaccggt catgggtcta ataatggagc gggtcgagct
     1081 ggagacgcct cagcctatcg agtgcgacgc agaccacccc ctggtgagtc gagcccaggc
     1141 gggtgacgtt gccgccttcg aggcgctggt ccggcggtac gaacgatggg tgttcaccct
     1201 ggctctgcgc atgctggggg atcgcggcga ggccgaggac atggcccagg aagtgtttct
     1261 caagacctac cggggcctca aaggcttcaa ggggacctcc cgcttctcca cgtggctcta
     1321 tgccatcgcc agtcaccatt gcctgaatta tctgcgggcc cgccgacgcc ggccggaggc
     1381 gtccgggggc tgggcagagg gagaggacgg cgaggaactc caggcggccg atcgccttcc
     1441 ggatggtgcc ccgcgggccg acgctctcct ggagcgggcg gatctggccc ggatcgtcca
     1501 gaccgaactg acctacttga ccgaggacca ccggctgatc ctgatcctgc gagacattca
     1561 ggggctgacc tatggggaga tcgccgaggc tctgggcgtg gagcttggaa cggtccggtc
     1621 ccggctccac cgcgcccgag cagagatgcg agagcggctg gcagtgcacc tccaaactgg
     1681 gtgacgcatg atctgcgacg aggtccggga agccttttcc gaattctacg atgacatgct
     1741 gtcaggcacc cgtttgacag acctcaccca gcatctggac gagtgttcgg aatgtcgggc
     1801 ggaatgggag gcgttctcga cgactatgca ggtcgtcacc cggctaggga tggccgaccc
     1861 atctcccggg tttgcggcga gggtccgcca gcagatcgag gccccgccat ggtgggggcg
     1921 gctcggccac agcctgttcg tcccgctgca tgtcaaggtg cccatccacg ccgtggccct
     1981 ggccctgctc tcgttcgccg gcctca
//
LOCUS       DBNGZC010000823         6357 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1833109, whole genome shotgun sequence.
ACCESSION   DBNGZC010000823 DBNGZC010000000
VERSION     DBNGZC010000823.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6357)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6357)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6357
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1833109"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="AC16G0_14125"
     CDS             <1..1201
                     /locus_tag="AC16G0_14125"
                     /inference="COORDINATES: protein motif:HMM:NF017686.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HIR5100786.1"
                     /translation="VVGLWLEPESPWLKAAALRIAAATPYGQAMVRTGLTRMMAGCRK
                     EALLSLLQEELGDPRVLDGFRPRRAAGGCHRACGPAITTHIFSGNVPGLPAISLVQAL
                     LVKSASLGKPASEELVFPAMFARSLAAVDSKLAASVAILPWPGGEGAIEHAAFAASEA
                     VVAYGSDPAVESIRGRVPASVRFVGHGHRLSFGVIGRESLDPGTLDELAERVAYDVSL
                     FDQQGCVSAHLLYVERGGAVPPDTFAERLASAMGAFEREMPRGKLSVEEAAAIQQGRA
                     EAELREMREEPVRLWASPHGTAWTVIYEDDPSFVASCLNRVVRVKAVRDLREIPSLVR
                     PFSRHLQTVGTAMSPERRVRLAEALAPFGACRVCAIGEMPHPPLTWHHDGGFSLLPLL
                     RWTAIEG"
     gene            1286..2059
                     /locus_tag="AC16G0_14130"
     CDS             1286..2059
                     /locus_tag="AC16G0_14130"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5100787.1"
                     /translation="MTAKVEARDIFSEFESPSGPMLALRHVDLTAQAGEFVTLVGESG
                     CGKTTLLRILAGLLPPTQGEVRIDGAPVTGPSSQVGIVFQRPVLLPWRTAMENALLPA
                     QVQGLPLDRAAAEANGLLASMGLGEFVDRFPRQLSGGMQQRVALARALLLQPGILLMD
                     EPFGALDAITREQMHLDLLQVWSARRPTILFVTHDIAEAVFLADRVVLLSPRPGTVRR
                     VFDVALPRPRVPDHRFTAEFAATCREVKHAMQMNGGAGG"
     gene            2056..2883
                     /locus_tag="AC16G0_14135"
     CDS             2056..2883
                     /locus_tag="AC16G0_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006216082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5100788.1"
                     /translation="MKTSQAVEPLLITAAPETSSRRIPWAGGKVLGPAILFLVFVGVW
                     EGTVRLRGIPPLVLPAPSLVWESLVTHTRSGLLLVHTGVTLSEILLGFLGGSVLGIGL
                     GIGVALSPRLRGLLHPYLIASQAMPKLALAPIFVMWFGFGILPKALITALIAFFPLFE
                     NTVTGLNEVESDALELFAALRASRWQTLCWLRLPNAVPYVVAGLRVAMILSVVGAVVA
                     EYVGANRGLGALIISSQGMMDTPLMFAVFVVLVALGVVLYEAIGLVERRCLARRYRE"
     gene            2941..3957
                     /locus_tag="AC16G0_14140"
     CDS             2941..3957
                     /locus_tag="AC16G0_14140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008880486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5100789.1"
                     /translation="MSRERTSTWSRRQFLRACGILGTAVATGWKRTAAEAPEKVRMAC
                     WRQPISEQANVFAAQEFGWFKEQGLDFEFVPGAGGGEALKHVLAGNADFAFTNVEPIL
                     FGAEQGAQVQGVYNIYPKNVFNVVSLKAKNITKPQELKGKRVGVYSMASGTRHNLLVI
                     LHSVGLKESDVEVVATGVLNFGPLLEGKVDATAATDTGLWAAQQKGLGEVKVIWARDY
                     LDIPTDVFAVRAETVQKKPGLIPRFLRAYRKGSQWMLDNPEKAAELAVKYATDGQDVK
                     RNLEIIKIRNASTLSQGTKKSGLGWFDIDLLRKVGKTYLELGLLKNPVDVDSLFTNRF
                     VREL"
     gene            complement(4055..4498)
                     /locus_tag="AC16G0_14145"
     CDS             complement(4055..4498)
                     /locus_tag="AC16G0_14145"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR5100790.1"
                     /translation="MTENGGPILRTPQAILARTNERIRGTFAGVLGMAFTHFAPDRVE
                     ATLEVREELKQPWGIVHGGAVMTLADTVAGAGAYLNAQGGQTVTVELKINLIGAVREG
                     RIRAVAVPVHRGRTTSVWETRITDEAEKLVAVALSTHLVLDRPSA"
     gene            5041..6018
                     /locus_tag="AC16G0_14150"
     CDS             5041..6018
                     /locus_tag="AC16G0_14150"
                     /inference="COORDINATES: protein motif:HMM:NF012449.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR5100791.1"
                     /translation="MNYQIVATLGPSSATESVWQAMLAAGVTAFRLNTSHLAPPDLDA
                     WVGRLGSFLAGRDSRPSLILDLQGSKWRLGQFPACELASGQSVALVCAASVDNSGVLP
                     VPHPDFFRAASGSSGEIVLNDAKLRLGLESQDGQSLHARVIMGGPIMPRKGITYAAST
                     YRQESLNDTDRAILARTQHLAGFRYALSYVKDAVEMANYRKQVGRPAYLIAKLERQPA
                     VDEAMQIAESADEMWLCRGDLGAELGMKAMAATVYRFSGRVTALPVPVLLAGQIFEHM
                     TEHPTPTRSEVCMAYAALMQGYHGLVLSEETAIGRDPVESCRMAALLRP"
     gene            6035..>6357
                     /locus_tag="AC16G0_14155"
     CDS             6035..>6357
                     /locus_tag="AC16G0_14155"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5100792.1"
                     /translation="MTDVRMFSGKTAFVTGSSRGIGRVIATHLANLGASVAIQGTSPT
                     SARAFGEADSLEAVARAIEAESGNKVLAVDGNLADPEVVRNLVGVIRAAFGRIDFLVN
                     CAGGD"
BASE COUNT         1015 a         1937 c         2207 g         1198 t
ORIGIN      
        1 cgtcgtgggt ctctggctcg agccggagtc gccctggttg aaggccgccg cccttcgcat
       61 cgccgccgcc acgccgtacg gccaggccat ggtgcggacg ggtctcacgc gcatgatggc
      121 cggctgccgc aaggaggcgt tgctcagtct cttgcaggag gagctgggcg atccaagggt
      181 gctggacgga ttccgaccgc ggcgtgcggc cggtggatgc caccgggcct gcggccccgc
      241 catcacgacc cacatcttct ccggaaacgt ccccgggctt cccgccatca gcttggtcca
      301 ggctctgctg gtcaagtcgg cgagtctggg caagccggcg tcggaggaac tggtctttcc
      361 cgcaatgttc gcccggtctc tcgcggcggt ggattcgaag ctcgcggcct ctgtggcgat
      421 cctcccctgg ccgggaggcg aaggcgcgat cgagcatgcc gccttcgccg cctccgaggc
      481 ggtggtggcg tatggcagcg acccagccgt cgagagcatt cggggcaggg tgccagcgag
      541 cgtccgcttc gtggggcacg gccaccgctt gagcttcggg gtcatcggtc gggaaagcct
      601 ggaccccggt acgctggatg aactcgcgga gcgcgtcgcg tacgatgtgt ccctcttcga
      661 tcagcaaggg tgcgtgtccg cccacctgct gtacgtggag cgtggggggg cggtcccgcc
      721 cgatactttc gccgagaggc tggcgagcgc catgggcgcc ttcgagcgcg agatgccccg
      781 cgggaagctg tcggtagagg aggccgcggc gatccagcag ggacgggcgg aggcggagct
      841 tcgcgagatg cgggaggagc cggtccggct gtgggcgagt ccccatggaa ctgcctggac
      901 ggtgatctac gaggacgatc cgagcttcgt agcctcgtgt ctgaaccggg ttgtgcgcgt
      961 caaggccgtg agagacctcc gagagatccc cagtctggtc cggccgttca gccggcatct
     1021 ccagacggtg gggacggcca tgagcccgga gcgccgggtc cgtctggccg aagccctggc
     1081 gccgttcggt gcctgccgcg tctgcgccat cggcgagatg ccccatccgc ccttgacctg
     1141 gcatcacgac ggcggtttca gcctgctccc cctgctgagg tggacggcga tcgaaggctg
     1201 aacccgggtt ccctgatcga cggcttgcca cattcagatt tggtcatttg tcattcgtca
     1261 ttcatctgag ccacaggtct ttaccgtgac ggcgaaggtc gaggcgcgcg atatcttcag
     1321 cgagttcgaa agcccgtcgg ggcccatgct ggcgttgcgc catgtggacc tcacggcgca
     1381 ggcgggcgag ttcgtgacgc tggtgggaga gagcggttgc gggaagacca cgctcctccg
     1441 gatcctggcg gggcttctcc cgccgacgca aggcgaggtg cggatcgacg gggcgccggt
     1501 caccggtccc agctcccagg tcggtatcgt gttccagagg ccggtcctgt tgccgtggcg
     1561 aacggccatg gagaacgcgc tgttaccggc gcaggtgcag ggtctccctc tggatcgagc
     1621 ggctgccgag gcgaatgggc tcctggcgag catgggtctg ggggaattcg tggaccgatt
     1681 cccgcggcag ctctcgggtg ggatgcagca gcgggtggct cttgcccgcg ccttgctcct
     1741 gcagcccggc atcctgctga tggacgaacc ctttggagcg ctggatgcta tcacccgcga
     1801 gcagatgcac ctggacctcc tgcaggtgtg gagtgcgcgc cggcccacca tcctgtttgt
     1861 cacccacgat atcgccgagg cggtcttcct ggcggatcgc gtggtgctgc tgagtccgcg
     1921 gcccggaacg gtgcggcggg tgttcgacgt ggccctgcct cgaccccgcg tgcccgatca
     1981 ccgcttcacc gccgagtttg cagcgacctg ccgggaggtg aagcatgcga tgcagatgaa
     2041 cgggggagcc ggaggatgaa gacaagccag gcggtcgagc cgctcctcat caccgcggcg
     2101 cccgagacgt catcccggcg aattccctgg gcaggcggga aggtcctggg cccggcgatc
     2161 ctgttcctgg tgttcgtcgg tgtgtgggag ggaacggtcc gacttcgggg cattcctccc
     2221 ctggtcttgc ccgccccgag cctcgtgtgg gagagtctgg tcacccacac caggagcggg
     2281 ctcctcctcg ttcacaccgg agtcacgctc tcggagatcc tgctgggatt tcttgggggg
     2341 agcgtcctgg gcatcggcct gggcatcggc gtcgccctct cacctcgtct gcgcgggttg
     2401 ctccacccct acctgatcgc atcccaggcc atgcccaaac tggccctggc ccccattttc
     2461 gtcatgtggt tcggcttcgg catcctcccc aaggcgctga tcacggcgct catcgccttt
     2521 tttcccctgt tcgagaatac ggtgacgggt ttgaacgagg tggaaagtga cgccctggaa
     2581 ctgttcgccg ccctgcgggc gagtcggtgg cagacgcttt gctggctgcg gctgccgaac
     2641 gcggtgccgt acgtggtcgc ggggcttcgg gtggccatga tcctcagcgt cgtaggagcc
     2701 gtggtggcgg agtatgtggg ggcgaaccgt ggcctgggag ccctaattat cagttcccag
     2761 gggatgatgg atacccccct gatgtttgcc gtgttcgtcg tgttggtggc cctgggcgtg
     2821 gtcctctacg aagcgatcgg cttggtggag cgacgctgtc ttgccaggcg gtaccgggaa
     2881 tgaggcgggt aagggcatag agcacagagc acagggcaca gggtaagaaa ggggtttgag
     2941 atgagtcgtg agcgaaccag tacgtggtcc cggcggcagt tcctccgggc gtgtggcatc
     3001 ctggggacgg cggtggcgac cgggtggaag cggactgcgg cggaggcgcc ggaaaaagtc
     3061 cggatggctt gttggcgcca gccgatctcc gagcaggcaa acgtctttgc ggcgcaggag
     3121 ttcgggtggt tcaaggagca gggtctggac ttcgagttcg tgccgggggc cggcggcggc
     3181 gaggcgctga agcacgtgct ggccgggaac gcggatttcg ccttcaccaa tgtggagccg
     3241 atcctgttcg gcgcggagca gggggcgcag gtccaaggtg tgtacaacat ctatcccaag
     3301 aacgtgttca acgtggtgtc gttgaaggcc aagaacatca ccaagcccca ggagctcaag
     3361 ggcaagcggg tcggcgttta cagcatggcc agcggcacgc ggcacaacct gctcgtcatc
     3421 ttgcattcgg tgggattgaa ggaatccgac gtggaggtgg tggcgaccgg cgtcctcaat
     3481 ttcgggcccc tgttggaagg caaggtggat gccacggccg caaccgatac cggcctctgg
     3541 gccgcgcagc aaaaggggtt gggcgaggtc aaggtgatct gggctcggga ctacctggac
     3601 atccccacag atgtgttcgc ggttcgggcc gagaccgtgc agaaaaagcc cggcctcatc
     3661 ccacgcttcc tgcgggccta tcggaagggg agtcagtgga tgctggataa tccggagaag
     3721 gcggcggaac tggcggtgaa gtacgccacg gacgggcagg acgtcaagcg caacctagag
     3781 atcatcaaga tccgcaacgc ctccacgctg agccagggga caaagaaatc cggcctggga
     3841 tggtttgaca ttgacctgct ccggaaggtg ggcaagacct atctggaact cggcttgctt
     3901 aagaatccgg tagacgtcga ttcgctcttc accaaccggt tcgtgcggga gctctgagag
     3961 agcctcccac cggctgtcct tgctcccgaa ttgggcagac ggcgagcctg aagagttctt
     4021 ggcgagcgat gctaccgtga aaatgggact tgggctatgc ggatggtcgg tcgagaacga
     4081 ggtgagtgga gagggcgacg gcgacgagct tttccgcctc gtcggtgatc cgcgtttccc
     4141 agacagaggt ggtccggcct cggtggacgg gaaccgccac cgcacggatc cttccctccc
     4201 gcacggcgcc gatgagattg atcttcagtt ccacggtcac ggtctggcca ccctgggcat
     4261 tcagataggc gcctgctccg gccacggtgt cggctaacgt catcactgcg ccgccgtgca
     4321 cgattcccca gggctgcttg agttcttccc ggacctccag tgtggcttct acccggtcgg
     4381 gggcgaagtg ggtgaacgcc atccccaaga ctccggcaaa ggtcccccga atccgctcgt
     4441 tggtccgggc aaggatcgcc tggggtgtcc tcaggattgg tcctccattc tccgtcatcc
     4501 cgacctcctc tcggcgtcga tacacaggcg attgggcagc tgggcgatct ggcgcctcga
     4561 ctcgtcagaa actccgagcg cagatgctcc agttgcccag ccgcctgcgt gccgcgcacc
     4621 gcctgccaag cgcgcgtatc ctagccggtg aggcttcgcc tggcaatcgg ggatgtggac
     4681 agccaggcaa cccggcgact ggatcccttg gcgtgtcgat ggttcgaggg tgccatccca
     4741 cctggttggg agatcgctcg gctgcttaac ctggcgactc agcaggggac ccctttggga
     4801 ttgacacccc gcgaaccctt cgatactctc ccgcccgaac aacccgaccc ccttccacat
     4861 ctctctgggc gaggctcccc tgagtctcca gccgtgcagc tttggcgctt gcgtttgtga
     4921 ttaatagtcc ctcgtagtct ggactcagac tgaaagccat ccgacgccca cgttgcttgg
     4981 aacagaagtg ccggagtacc gcgtcccgtg aatagcaccg atcggtctga ggggctaggg
     5041 atgaactatc agatcgtcgc gacgctcggg cccagcagtg ccactgaatc ggtgtggcag
     5101 gccatgcttg cggctggggt gacggcgttc cgcttgaaca cttcccactt ggccccgcca
     5161 gacctggatg cctgggtcgg ccggctcggc agcttcctgg ccggacggga ttcgcgaccg
     5221 tcgctgatcc ttgacctgca gggcagcaaa tggcgtttgg gtcagttccc tgcgtgtgag
     5281 ctcgcttctg ggcaaagtgt ggcgctggtg tgcgccgcgt cggtcgacaa ctccggagtg
     5341 ttgccggtcc cacatccgga cttctttcgg gccgcatctg gatccagtgg tgagatcgtg
     5401 ctgaatgatg ccaagctgcg tctcgggctc gagtcgcagg acggccagtc gttgcacgct
     5461 cgcgtgatca tgggcgggcc catcatgccc cggaagggga tcacgtacgc cgcctccacc
     5521 tatcgtcagg aatcgctgaa cgatacggat cgggcgatct tggcgaggac gcagcatctg
     5581 gcgggtttcc ggtatgcgtt gtcgtacgtg aaagatgccg tggagatggc gaactaccgc
     5641 aagcaggtcg gacgcccggc ttacctgatc gcgaagctgg agcgtcaacc tgcggtggac
     5701 gaggcgatgc agatcgcgga gtctgcagat gagatgtggc tgtgtcgggg cgatctcggc
     5761 gccgagttgg ggatgaaagc gatggccgcc accgtctacc gcttctcggg acgtgtgact
     5821 gcgttgccgg tgccggtgct cctggcggga cagatcttcg agcacatgac cgagcacccc
     5881 actcccaccc gatcggaagt ctgcatggcc tacgccgcac tgatgcaggg ttatcacggc
     5941 ttggtcttgt cagaggagac ggccatcggc cgcgacccgg tcgaaagctg ccgcatggcg
     6001 gcacttctgc gaccgtagct caggaggcga caggatgacg gacgtaagga tgttttcagg
     6061 gaagacggcg ttcgtgaccg ggtcgtcccg aggcatcggt cgggtcatcg cgacccacct
     6121 ggcgaacctt ggtgcttcgg tggccattca gggaacttcg cctacctcgg ctcgggcttt
     6181 tggggaggcc gattccctcg aggccgtggc ccgggcaatc gaggcggagt ccgggaacaa
     6241 ggtcctggct gttgacggaa acctcgccga cccggaggtc gtccggaacc tggtgggggt
     6301 gatccgggcc gccttcggcc gaatcgattt cctggtcaac tgcgcgggtg gcgacat
//
LOCUS       DBNGZC010000824         2305 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_461457, whole genome shotgun sequence.
ACCESSION   DBNGZC010000824 DBNGZC010000000
VERSION     DBNGZC010000824.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_461457"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="AC16G0_14160"
     CDS             <1..818
                     /locus_tag="AC16G0_14160"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR5100793.1"
                     /translation="KVSFSIRSLLIAVWSGALFLGLLGAAVWTWQGVRSEWPPGEDTS
                     LEGLQVFGTLPEFSLIERSGKRVTLAEFRGKVWVADFIYTHCTDTCPLQSAELARLQA
                     DLATEPDVRLVSITVDPAEDTPEVLAEYAARFGADRERWLFLTGPKRAIYTLAQRGFL
                     LNVEDPGDAVPSLPVAQPPSARPRPRPEPTPAPQSPPGRIEGSSALEALSSPIEATPV
                     MVHPGHLGPTFMHRPWFVLVDSQARIRGYYRSEDGKAMYRLRRDVRVLLGRKT"
     gene            complement(901..1500)
                     /locus_tag="AC16G0_14165"
     CDS             complement(901..1500)
                     /locus_tag="AC16G0_14165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5100794.1"
                     /translation="MLHKGKRVTRPVPSRQKAGHDFFGHGEEIRAALDRRLPRRPVKA
                     LDVGTGFGRNAVFLARHLPQGSHVWSIDPSEDSLKMGEEAVQKAGLADHVSLVPGAVE
                     RLPFADAEFHLAVGVMLFHHLVAVPPALREMARVTRPGGKLLLLDWGPTAHLLPFAIE
                     HRVEDFFTAHAVEKMVREVGLQPVVEQHPMWYVVEAAVE"
     gene            complement(1582..2256)
                     /locus_tag="AC16G0_14170"
     CDS             complement(1582..2256)
                     /locus_tag="AC16G0_14170"
                     /inference="COORDINATES: protein motif:HMM:NF046841.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6982 domain-containing protein"
                     /protein_id="HIR5100795.1"
                     /translation="MDTETTCTFELPAETFPVAIRVVSGGLPIVGAEVLIREVDPNFR
                     MTRKDEGAQFFLTPGAYTVVVTHGDALMKEAIHVSEVETEFTLDLSRQIAVRANLVVV
                     RYLDGRLVKGVTEDFAPGKSEFSVIQQHGKRVTIRGFEGVKAVFFVKSLDGNRLYAEQ
                     KDFANANQFGQKTIVVFRDKEEMSGYTLIGHADQPEFFLYPVDPKSNNLKVLVIRDSV
                     VEIRFA"
BASE COUNT          417 a          766 c          680 g          442 t
ORIGIN      
        1 ggaaagtcag cttctcgatt cgctcactgc tgatcgcggt ctggtccggg gcgctcttcc
       61 tcggcctctt gggcgcagcc gtctggacct ggcagggcgt gcggtccgaa tggccgccgg
      121 gggaggatac ctcgctggag ggcctccagg tcttcggcac gcttccggaa ttttcactta
      181 tcgagcggag tggaaaacga gtgacgctgg ctgagttccg gggtaaggtc tgggtcgccg
      241 attttatcta cacccattgc acggacacct gccccctcca gtctgcggag ttggcgcgct
      301 tacaggccga cctggcaacc gagccggacg tgagactcgt ctccatcacg gtggatccgg
      361 cagaggacac cccagaggtt cttgccgaat acgcggcccg ctttggggcg gaccgggagc
      421 gttggctctt cctgacgggc ccgaagaggg ccatctacac cttggcccaa aggggcttct
      481 tgctgaatgt ggaggaccct ggggatgccg tgccgtcgct ccctgtcgcg cagccgccgt
      541 cggccaggcc cagaccgagg cccgaaccca ccccggcccc ccaatctcca ccgggtcgga
      601 tcgagggatc atcggcgctc gaggccttgt cctcgccaat cgaggccaca ccggtgatgg
      661 tccatcccgg acaccttggc ccgacattca tgcaccgccc ttggtttgtc ctggtggaca
      721 gccaggctcg cattcggggc tattaccgga gcgaggatgg gaaggccatg taccgcttgc
      781 gcagggacgt gcgggtcctt ctcggaagga agacataacc gctgcaggag cgtgcgattt
      841 accggctggg atgacgaatg cttgtcccca ccctcccacg gcttgagcct cgaatagaac
      901 tcattccacc gctgcctcca ccacatacca catcggatgc tgctccacaa ccggctggag
      961 gccgacttcc cggaccatct tctccaccgc atgcgcagtg aagaagtctt ccacgcggtg
     1021 ctctatggcg aagggaagca ggtgggccgt cggcccccaa tccaggagga gcagctttcc
     1081 gccaggccgg gtcacccgcg ccatctcccg tagcgccggc ggcaccgcga ccagatggtg
     1141 aaacagcatc acgcccacgg caaggtggaa ctcagcgtct gcgaatggca accgctccac
     1201 cgcacccggg accagcgaga cgtgatctgc caggccggcc ttctgaaccg cctcctctcc
     1261 catcttcagc gaatcctcgg agggatctat ggaccagacg tggctcccct gcggtaggtg
     1321 ccgtgccagg aagaccgcat tccgtccgaa ccccgtacct acatccagcg ccttcaccgg
     1381 ccgccggggc agccgtcggt ccagggccgc ccggatctcc tccccgtggc caaagaagtc
     1441 gtggcccgcc ttctgccgtg acgggacggg gcgcgttaca cgctttccct tgtgtagcat
     1501 cttcacgccc gcattggaca ccgaacgggg tcgaatggga ggcggtctgc cggctgaaat
     1561 cagccggcgg tcgccgatgc atcaggcaaa gcgaatctcg accaccgaat ccctgatgac
     1621 caacaccttg aggttgttgc ttttgggatc gacaggatag aggaagaatt ccggctgatc
     1681 cgcgtggccg atcaaggtgt agccgctcat ctcttccttg tcgcgaaaga ccacgatggt
     1741 tttctgaccg aactgattgg cattggcaaa gtccttctgt tccgcgtaga gccgattccc
     1801 gtccaggctc ttcacaaaga aaacggcctt cacgccctcg aagccccgga tggtcactcg
     1861 ctttccgtgt tgctggatta cgctgaactc tgatttcccg ggggcaaaat cctcggtgac
     1921 ccccttgacc aaccttccat ccaggtagcg gaccaccacc agattcgccc tgacggctat
     1981 ctggcgggag aggtccaggg tgaactcagt ttccacctcg ctgacgtgga tggcttcctt
     2041 catcagggcg tcgccatgtg tcaccacgac cgtataggcg ccgggcgtga ggaagaattg
     2101 ggcgccttca tctttgcgcg tcatcctgaa attgggatcc acctctcgga tcaagacctc
     2161 cgcccccacg atgggcagcc ctcccgagac aacccggatc gccaccggga acgtctcggc
     2221 cggcaactcg aaggtgcagg tggtctccgt atccacattg acttgtatcc tctgcgatcg
     2281 ttcgaacgag ccttacacgc ccttg
//
LOCUS       DBNGZC010000825         6354 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_169661, whole genome shotgun sequence.
ACCESSION   DBNGZC010000825 DBNGZC010000000
VERSION     DBNGZC010000825.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6354
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_169661"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="AC16G0_14175"
     CDS             <1..568
                     /locus_tag="AC16G0_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5100796.1"
                     /translation="RLCDQHGVLLILDEVQTGMGRTGTLFAYEHWGVEPDILTLAKAL
                     AGGVPIGAMLAKEAVAQSFVPGSHASTFGGTPFVSAVAHAVVTAMIQEDLPGNAARVG
                     KYFLDRLADLKGRHGVVTGVRGLGLILGLEVSVPARTIVSACMARGLLVLTAGDNIVR
                     FVPPLIISEADVDRAVGILDEVLTGLGK"
     gene            599..1531
                     /gene="argF"
                     /locus_tag="AC16G0_14180"
     CDS             599..1531
                     /gene="argF"
                     /locus_tag="AC16G0_14180"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR5100797.1"
                     /translation="MNRHLITLDDLTPADVESLFELAASLKTKRRQGVPHAHLTGKTL
                     ALVFEKPSLRTRVTFEAGMTQLGGHAIYLAPADIRLGERETVQDAARNLERWVDGIVA
                     RTFSHQMVERLARYSRIPVINGLTDSHHPVQILCDLFTLWEKRGSLAGLKVAFIGDGN
                     NVCHSWLQGAAKMGLCFTLACPPGYEPDPTVMAQAQEQAKAGGATLAVSHDPGAAAQG
                     ADVLYTDVWASMGQEDERARRLRDFQGYQIDGALLRLANPEVLVMHCLPAHRGEEIAG
                     EVIDGPHSIVFDQAENRLHMEKAILVSFMGGRTN"
     gene            1623..2420
                     /locus_tag="AC16G0_14185"
     CDS             1623..2420
                     /locus_tag="AC16G0_14185"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5100798.1"
                     /translation="MRLAEKVALVTGGGAGIGRAIVLALAREGADLLILDIRLDNAEA
                     VAKEVRALGRRAAALQVDVTQQARIEQAIADGIRQFGRIDILVNNAGVNPGLGLPFTR
                     QTEGDWDTVFNVNTKSVFFTCKAIAPHFMERRFGKIVNIASIAGPMSSQTMPSYSVSK
                     MGVVTFTRIVAKDLAAYNVNVNAVCPGLLWTDMWKNIGEVIRGTNPAYAGLTPRQMFD
                     KRVEEWIPLKREQTPEDIGEATVFLVSEAARNITGQTLVVDGGAYMH"
     gene            2493..3878
                     /gene="argH"
                     /locus_tag="AC16G0_14190"
     CDS             2493..3878
                     /gene="argH"
                     /locus_tag="AC16G0_14190"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR5100799.1"
                     /translation="MRRKKLWGGRFGTDTNRQVEAFTASIHYDQRLAPHDIQGSIAHA
                     RMLGKCGILTKDETEAIVTGLAEIGEEIARGAFRIDPALEDIHTHVERRLIEKIGEVG
                     GKLHTARSRNDQVALDLRLYLREAIGQIRDQVRTLQDVLVAKAEVNLDTILPGYTHLQ
                     RAQPILLAHHLLAYFEMFQRDRDRLADCLVRVNVLPLGAAALAGTTLPIDRAYTAKLL
                     DFPRIAANSEDAVSDRDFAVEFLACGAILAMHVSRLAEELVLWASPEFGFIELPDAFA
                     TGSSIMPQKKNPDVAELARGKTGRVFGALVSLLTLLKGLPLSYNRDLQEDKEPVFDAV
                     DTIQQTLAVLPPMLKAINYRPERMRAAAADGFLNATDLADYLVTKGVPFRQAHEIVGR
                     IIQECLETNRRLEDLPLERLRHFSQSFGADVHKHISLEACVERRRSAGGTSSRLVGEA
                     VRQAKHTLRHP"
     gene            3989..4966
                     /locus_tag="AC16G0_14195"
     CDS             3989..4966
                     /locus_tag="AC16G0_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR5100800.1"
                     /translation="MAPAAVSTIRAEAEDGAIVVSWVRPSRNEDRSPLTDLAEFRLFR
                     AVSPAAEGAAAGRPTFSLLATVRADRPENASVEGDRIAFRDGGGSVGLLPDTRYTYRV
                     QAVNRKGTAGPPSVEISVDFLLPPPAPAGLSASVGDGMVDLSWQAPPQPGPPGTSLPR
                     GYNLYRAEGSEGYGGEPINGSPIAETRFRDSGVVNDHTYRYVVRSVAGGRPPWRESQD
                     SNEVTAMPMDFTPPAPPRGLVAIPGPGGIALSWEPNAEPDLLGYFIYRRELPGLTSSR
                     LLQAPIPATSYLDRTAQSGKSYVYTVTAVDRSRHRNESDPSAEASASQP"
     gene            complement(5050..5607)
                     /locus_tag="AC16G0_14200"
     CDS             complement(5050..5607)
                     /locus_tag="AC16G0_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011871317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HIR5100801.1"
                     /translation="MKARPPKSSPLALTIGHSTRTLQSFLDLLTAHGVERVVDVRTVP
                     RSRHNPQFNQDALPAALAAVGIRYAHLPGLGGLRHTRGDSVNIGWRNASFRGFADYMQ
                     TPAFVESLQEVLDFISGEQVVLMCAEAVPWRCHRSLLADALTVRGVRVEHILSGVRCQ
                     PHTLTPFARVEGTRITYPAAPEAEG"
     gene            5720..6187
                     /locus_tag="AC16G0_14205"
     CDS             5720..6187
                     /locus_tag="AC16G0_14205"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR5100802.1"
                     /translation="MKRSAYPYVQEGGGTSSRKPNNLGVTAAMADSQERRGAIRAVVP
                     GRIGGRVRATLEIRLLDLSLSGARVEHQNLLRPGFVCALELPPTLGSLSLSVRVVRSI
                     VVGTEQNEAGERLLRYESGLAFIGLTAKQHATLSSILERLNPGGGLGEGKIFL"
BASE COUNT         1092 a         2102 c         2140 g         1020 t
ORIGIN      
        1 gcggctgtgc gaccagcacg gcgtcctcct gatcctggac gaggtgcaga cggggatggg
       61 tcgcacgggg acgctgttcg cctacgagca ctggggggta gagccggaca tcctgacctt
      121 ggcaaaggcg ctggcgggtg gggtgcccat cggggccatg ttggccaagg aggccgtggc
      181 ccagagcttt gtccccggct ctcacgcctc gaccttcggg gggaccccct tcgtctcggc
      241 cgtggcccac gccgtggtga ccgccatgat ccaggaggat ctcccgggca acgccgcccg
      301 cgtgggaaaa tacttcctgg acaggctggc cgacctgaag gggaggcacg gcgtcgtgac
      361 cggggtgcgg ggccttggtc tcatcctggg gctggaggtc tcggtgcccg cccggaccat
      421 cgtctccgcc tgcatggcgc ggggtctcct cgtcctcacg gccggcgaca acatcgtgcg
      481 gttcgtccca cccctcatca tcagcgaggc cgatgtggac cgggccgtcg ggatcctcga
      541 cgaggttctc accggcctcg gcaagtagct cggtttttcc ttggcaagga gtcgtgggat
      601 gaatcgccat ctgatcacgc tggacgacct gacgccggcc gacgtggaga gccttttcga
      661 gttggcggcg tccctgaaaa ccaagcgacg gcagggggtc ccgcacgctc acctcacagg
      721 caagaccctg gccctggtct tcgagaagcc ctccctcagg acgcgggtca ccttcgaggc
      781 tgggatgacg cagctcggcg gccacgccat ctacctggct cccgccgaca ttcgcctggg
      841 agagcgcgag acggtgcagg atgcggcccg aaacctggag cggtgggtgg acgggatcgt
      901 ggcccgcacc ttcagccacc agatggtcga gcggctggcc cgctactccc ggatccccgt
      961 gatcaacggc ctcacggaca gccaccaccc ggtccagatc ctgtgcgatc tcttcaccct
     1021 gtgggagaag cgggggtccc tggccggtct gaaggtggcc ttcatcggcg acggcaacaa
     1081 cgtgtgccac tcctggctcc agggggcggc caagatgggg ctgtgcttca cgctggcctg
     1141 cccgccgggg tacgagcccg atccgacggt catggcccag gcgcaggagc aggccaaggc
     1201 cggcggggct accctggcgg tgagccacga cccgggggct gcggcgcagg gggccgacgt
     1261 cctctacacc gacgtctggg ccagcatggg ccaggaagac gagcgggcgc gacgcctccg
     1321 ggacttccag gggtaccaga tagacggcgc gctgctccga ctcgccaatc ccgaggtgct
     1381 ggtgatgcac tgcctgccgg ctcaccgcgg tgaggagatc gccggcgagg tgattgatgg
     1441 gccacacagc attgtcttcg atcaggcgga gaatcgcctg cacatggaaa aggccatcct
     1501 ggtcagcttc atgggcggga gaactaactg aagaaatcgt tcgacggttc gacggtacgg
     1561 ctgttctgaa gggccgaaca gcagagcggc cgagcactct ttctcggccg gggagcgtgg
     1621 ggatgcggct ggcggaaaaa gtggcgttgg tcaccggcgg cggcgccggg atcggtcgcg
     1681 ccatcgtctt ggcgctggcg cgcgaggggg ccgacctcct gatcctcgac atccggctgg
     1741 acaacgcgga agcagtggcc aaggaggtcc gggctctggg ccggagggcg gcggctctcc
     1801 aggtggacgt tacccagcag gcccggatcg agcaggccat cgcagacggt atccggcagt
     1861 tcggccggat cgacatcctg gtgaacaatg cgggggtcaa cccgggtctc ggcttgccat
     1921 tcactcgcca gaccgaaggc gactgggaca cagtcttcaa cgtcaacacg aagtcggtgt
     1981 tcttcacctg caaggccatc gcaccgcact tcatggagcg gcggttcggg aagatcgtca
     2041 acatcgcctc catcgcgggc cccatgtcca gccagaccat gccgtcctac agcgtgtcca
     2101 agatgggggt cgtgaccttc acccggatcg tggccaagga cctggcggcc tacaacgtga
     2161 acgtgaatgc cgtctgtccg ggtctcctct ggaccgacat gtggaagaac atcggtgagg
     2221 tcatccgggg gaccaatccc gcctacgcgg gcctcacccc tcgccagatg ttcgacaaga
     2281 gggtggagga gtggatcccg ctgaagcggg agcagacgcc cgaggacatc ggcgaggcga
     2341 cggtcttcct ggtctccgag gcggcgcgga acatcaccgg ccagaccctg gtggtggacg
     2401 gcggggcgta catgcactga aaaccagctg aacgccctgc ggctcagaat ccgcgcgctc
     2461 gcccagaagc ggcagggctg aatggatcac ccatgaggcg caagaagctc tgggggggac
     2521 gcttcgggac cgacaccaac cgtcaggtgg aggccttcac ggcctcgatc cactacgacc
     2581 agcgtctggc accccacgac atccagggga gcatcgccca cgcccggatg ctcggcaagt
     2641 gcggaatcct caccaaagat gagacggagg ccatcgtgac gggccttgcc gagatcggcg
     2701 aggagattgc gcgcggtgcc tttcgcatcg atccggccct ggaagacatc cacacccacg
     2761 tcgagaggcg gctcatcgaa aagatcggcg aggtgggagg aaagctccac accgcgcgga
     2821 gccggaacga ccaggtggcc ctggacctgc ggctctacct ccgggaggcc atcggtcaga
     2881 tccgggacca ggtccggacg ctgcaggatg tcctggtggc caaggcggag gtcaacctgg
     2941 acaccatcct gccgggctac acccacctgc agcgggccca gcccatcctc ctggcccacc
     3001 acctgcttgc gtacttcgag atgttccagc gcgaccgcga ccggctcgcc gactgccttg
     3061 tccgggtgaa cgtcctgccc ctgggggcgg cggcattggc ggggacgacc ctgcccatcg
     3121 accgagccta cacggcgaag ctgctcgact ttcctcggat tgccgccaac agcgaggacg
     3181 cagtctcgga ccgggatttc gccgtggagt tcctggcgtg cggcgcgatc ctggccatgc
     3241 acgtctcccg gctggcggag gagctggtcc tgtgggccag tccggagttc ggcttcatcg
     3301 agcttcccga cgccttcgcc accgggtcca gcatcatgcc gcagaagaag aatccggacg
     3361 tggccgagtt ggcgcgtggg aagaccggtc gggtgttcgg cgcgctggtc agcctgctca
     3421 ccctgctcaa ggggctgccc ttgagctaca accgcgatct ccaggaggac aaggagccgg
     3481 tcttcgacgc cgtggacacg atccagcaga cccttgccgt cctgcccccc atgctgaagg
     3541 cgatcaatta ccgtcccgag cggatgcggg ccgccgccgc cgacggtttc ctgaacgcca
     3601 cggatctcgc cgactatctg gtcacgaaag gcgtgccgtt ccgccaggcg cacgaaatcg
     3661 tgggccgtat catccaggag tgcctggaga cgaaccgccg cctggaggac ctcccactgg
     3721 aacgtctgcg ccatttctcc cagtccttcg gggcggacgt gcacaagcac atcagcctgg
     3781 aggcctgcgt ggagcgacgg cggtctgccg gcggcaccag ctccaggctg gtgggcgagg
     3841 ccgtccgcca ggccaagcac acgctgcgcc atccatgaac cgaccgcccc gccccgtcct
     3901 ctccgcgtgt ctctttgtct gggttgcact cctcgggttg ctcctccccg cctgcgggcg
     3961 caaggggccg cccgttccac ctcgtccggt ggcccccgcg gccgtgagca cgatccgggc
     4021 ggaagcggaa gacggcgcca tcgtggtgag ctgggtccga ccgagccgga acgaggaccg
     4081 gtcgcctctc acggatctcg ccgagttccg tctcttccgt gccgtcagtc cggccgcgga
     4141 gggggctgcc gcaggacggc cgacattctc ccttctggcg acggtgcggg ccgaccggcc
     4201 ggagaatgcc agcgtcgagg gggacaggat cgccttccgg gacggcgggg gaagcgtcgg
     4261 gctgctgccg gacacgcggt acacctatcg tgtgcaggcg gtgaatcgga aagggactgc
     4321 cggcccaccg tcggtggaaa tctcggtgga cttcctgctg ccgccgccag cacctgccgg
     4381 cctttccgcc tcggtggggg acggcatggt ggatctttcc tggcaagcgc ccccccagcc
     4441 gggacctcca ggcacttcgc tccccagggg gtacaacctg tatcgggccg aggggtcgga
     4501 agggtacgga ggcgagccca tcaacgggag cccgattgca gagacacgct tccgggacag
     4561 cggcgtggtg aacgaccaca cataccgcta cgtggtcagg agtgtggcgg gcgggcggcc
     4621 cccctggcgt gagagccagg attccaacga ggtcacagcc atgccgatgg atttcacgcc
     4681 gcctgcgccc ccccgcggtc ttgtggcgat ccccgggccg ggagggatcg ccttgagctg
     4741 ggagcccaat gccgagccgg acctcttagg gtacttcata tatcgccggg aattgccggg
     4801 tctgacgagc agccgtctcc tccaggcgcc gatccccgcc acgagctatc tcgaccgaac
     4861 ggctcagtcc ggcaagagct acgtgtacac cgtgaccgcc gtggaccgct cgcgtcatcg
     4921 aaacgaaagt gatccctccg ccgaagcctc cgcttcacag ccctgaaggg acatgcggca
     4981 tggcgtattg cttatcgcct atggcaagga cccccgcagc acttgccatg cgccatacgc
     5041 gatctgccgt catccctccg cctccggcgc agccggatac gtgatgcggg taccctccac
     5101 gcgcgcaaat ggcgtgaggg tgtggggctg acaacggacc ccgctcagga tgtgctcgac
     5161 ccggacacct cgcaccgtga gcgcgtcggc gaggagggag cgatggcagc gccatggcac
     5221 cgcctcggcg cacatcagga cgacttgctc acccgagatg aagtcgagca cctcttgcag
     5281 gctctccacg aatgccggcg tctgcatgta gtccgcaaag ccacgaaacg aggcgttgcg
     5341 ccatccgatg ttgactgaat cgccccgcgt atgccggagg ccgccgagac ccggcaggtg
     5401 cgcgtaccgg attccgacgg cggcgagcgc cgccggcaag gcatcctggt tgaactgagg
     5461 gttgtgccgc gagcgcggca ccgtacgaac gtccaccacc cgctccacgc cgtgcgccgt
     5521 gagcaggtca aggaagctct ggagggtccg cgtggaatgg ccgatggtga gcgcgagggg
     5581 cgagcttttc gggggacggg ccttcatgcc agagacctcg ggctgtcccg ctgccggagg
     5641 agagcacgca acgggaaagc cgccgggcgg cattgaaagt tcctcggacc gaataggtgg
     5701 agaagaaggc ttgacaacgg tgaaacggtc cgcataccct tacgtccaag aggggggtgg
     5761 tacttcaagc cggaagccca acaacttggg tgtcaccgcg gccatggcgg actcgcagga
     5821 acgacgtggc gccattcgtg ctgtcgtccc tggacggatt ggcggccggg tgcgcgcaac
     5881 cctggaaatc cgcctcctcg atctcagtct cagcggagcc cgagtcgagc accagaacct
     5941 tctgcgtccc ggcttcgtct gcgccctgga gcttcccccc actctcggct cgctctcgct
     6001 ctccgtccgt gtcgtccgga gcattgtcgt cggcacggag caaaacgaag caggcgagcg
     6061 gctgctgcgg tatgaaagcg gcctggcgtt catcggcctg acggcgaagc agcacgccac
     6121 cctctcgagt atcctggaac gcctgaatcc tggaggcggt ctgggggagg gaaaaatctt
     6181 cctctgattc ccctccttcg ttcgtgttcc tcctttcctt ccctccaagc aagtctaccg
     6241 caggaatcac ccgaatcgat caaattcttc actgcaatgc tcaccacgct cgtagaggta
     6301 ggcgaggcgg cattattcgg gatgcgtcga gccggggaac gcggcgagca cggc
//
LOCUS       DBNGZC010000826         3595 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_72086, whole genome shotgun sequence.
ACCESSION   DBNGZC010000826 DBNGZC010000000
VERSION     DBNGZC010000826.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_72086"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="AC16G0_14210"
     CDS             <1..1365
                     /locus_tag="AC16G0_14210"
                     /inference="COORDINATES: protein motif:HMM:NF005686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR5100803.1"
                     /translation="AVLARIDLVNPQVNAYVTLAREAALHAARTATSALRRRAALPPL
                     HGIPVSIKDLTPTKGIRTTWGSKIFENHVPDEDALIVQRLKAAGAIVVGKTNTPEFGA
                     GANTFNAVFGATRNPWNPALTCGGSSGGAAVALATGMGPIAQGSDLGGSLRIPAAFCG
                     VVGFRTTPGLVPAYPRELAWDSLSVQGPMARSVADVALMLSVIAGPDDRAPISYAVDT
                     REFLRAVRAPSVKGWRVAWTPDLAGLIPVEAEVARVAEGATQIFRSLGAKVESASPDF
                     RDVNEIVLATRGLSMVAQHAEKLPGWRDQMQKGLVWNIEQGLRLTPQEIGRAERLRTD
                     LWHRVRTFMQTRDLLILPTVAVPPFPVEQPYPTAINGRALENYTQWFFLTYAITLTGL
                     PAISIPCGFTQSGLPVGLQIVGRRRQEAAVLRAAAAFEAASPWRDRVPSIIQECHGPK
                     TGRG"
     gene            1517..3187
                     /gene="ilvD"
                     /locus_tag="AC16G0_14215"
     CDS             1517..3187
                     /gene="ilvD"
                     /locus_tag="AC16G0_14215"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR5100804.1"
                     /translation="MRSDTVKKGYERAGHRSLLRAVGLKEGDFSKPFIGVANSYVGII
                     PGHVHLQEVGRIVREAISAAGGVPFEFNTIGVDDGIAMGHSGMRYSLPSRELIADAVE
                     TMVMAHCFDGLVLIGNCDKIVPGMLMAAMRVNIPALYVSGGPMAAGKLADGKAIDLAS
                     VFEGVGAYSAGKIDEAALLELERKGCPTCGSCSGMFTANSMNCLAEALGMALPGNGTV
                     LAVDEGRKELYRTAGRQILKLVEMDLKPRDIVNRESVDNAFCLDMAMGGSTNTVLHTL
                     AIAHEAGVPYSLDRINQLSDRVPYLCKVSPAGHHHVEDVNRAGGISAILKRLAEKGDL
                     LRLERPTVTGNTLGEVIRHASVKDPEVIRPLENPYRQTGGLAVLFGNLAPEGGVVKAG
                     GVAPEMLRHEGPAVIFESQDDALAGILAGKVKAGDVVVIRHEGPRGGPGMQEMLSPTA
                     AIIGRGLGNSVSLITDGRFSGATHGGCIGHISPEAAAGGPIGLLKAGDRIRIDIDKRL
                     LEVELSEAELKVRRAAWRPPEPRVKTGWLYRYSRMVTSGSQGAVLKAD"
BASE COUNT          583 a         1125 c         1312 g          575 t
ORIGIN      
        1 gcggtcctgg cgcgcatcga cctggtcaat ccgcaggtca acgcctacgt gacgctggcg
       61 cgggaggccg cgctccacgc agcacggacg gcgacaagtg cgctcaggcg ccgggcggca
      121 cttcccccgc ttcacggcat tccggtttcc atcaaggacc tcaccccgac caagggcatc
      181 cggaccacct gggggtcgaa gatcttcgaa aaccatgtgc ccgatgagga cgcgctgata
      241 gtccagcgcc tcaaggcggc cggggcgatc gtggtcggca agaccaacac gccagagttc
      301 ggagcgggtg ctaatacctt caatgcggta ttcggcgcca cgcgcaatcc ctggaaccct
      361 gccctgacct gcggcggatc cagcggaggc gccgccgtgg ccctggccac gggcatgggg
      421 ccgatcgccc agggctcgga tctgggcggt tcgctgcgga ttcccgctgc cttctgcggg
      481 gtggtaggct tccggacgac cccgggtctt gtgccggcct acccgaggga gctggcctgg
      541 gattcgctct cggtccaagg ccccatggcc cgcagcgtgg cggacgtggc gctaatgctg
      601 tcggtgatcg cgggaccaga cgaccgggca ccgatctcct atgccgtgga cacgcgggaa
      661 ttcctcaggg ccgtccgggc gccgtcggtg aagggctggc gggttgcctg gacgccggat
      721 ctggccgggc tgattccggt ggaggccgag gtggcgcggg ttgccgaggg ggcgacgcaa
      781 atcttccgct ccctcggggc caaggtggag agcgccagtc cggactttcg cgacgtgaac
      841 gagattgtcc tggccacccg cgggctgtcc atggtcgctc agcatgcgga gaagcttcca
      901 ggctggcggg atcagatgca gaaggggctg gtctggaaca tcgagcaggg cctgcgcctt
      961 accccgcagg agatcggccg ggcggaacgg ctccgcacgg acctctggca tcgggtccgg
     1021 accttcatgc agactcgcga cttgctgatc ctgcccacgg tggcagtgcc gcccttcccg
     1081 gtggagcagc cctatcccac ggcgatcaac ggcagagcct tggagaacta cacccagtgg
     1141 ttctttctga cctacgcgat cacgctgacg ggcctgccgg cgatctcgat cccgtgtggc
     1201 ttcacccaga gcggcctgcc ggtggggctc cagatcgtgg ggcgacgccg gcaagaggcg
     1261 gcggtgttgc gcgccgcggc agccttcgag gccgcatccc cgtggaggga tcgggttccc
     1321 tccatcattc aggaatgcca cgggccgaag accggaagag gatgagggca ggcgaggttg
     1381 aaaatcttgc ccggccctgt caaacatggt agcttacaac cctttcgcga cgacaggggc
     1441 gaggggaacc atatttgtct gcagcgaggc aggtcgcggc cgtgccgcga ggcgtgcggt
     1501 ctgagggagg agtgcaatgc gtagtgacac agtcaagaag gggtatgaga gggcgggcca
     1561 ccggagtttg ctccgggcgg ttgggctgaa agagggcgac ttctccaagc cgttcatcgg
     1621 cgtggccaac tcgtacgtgg ggatcattcc gggacacgtt cacctgcagg aggtgggccg
     1681 gatcgtccga gaggcgatct ccgcagcggg gggcgttccc ttcgagttca acaccatcgg
     1741 ggtggacgac ggcatcgcca tgggacactc ggggatgcgc tactccctcc ccagccgcga
     1801 gctcatcgcc gacgcggtcg agaccatggt catggcccac tgcttcgacg gcctggtcct
     1861 gatcgggaac tgcgacaaga tcgtgccggg gatgctcatg gccgccatgc gggtgaatat
     1921 ccccgcgctc tatgtgagcg ggggacccat ggcggccggg aagctcgccg acgggaaggc
     1981 catagacctg gccagtgtgt tcgaaggggt cggggcgtac tccgccggga agatcgacga
     2041 ggcggccctc ctggagctgg aacggaaggg ctgtcccacc tgcggctcgt gctccggcat
     2101 gttcacggcg aactccatga actgcctggc ggaggcgttg ggcatggccc tgcccggcaa
     2161 cgggacagtc ctggcggtgg acgagggacg gaaggagctg taccggacgg cgggtcggca
     2221 gatcctgaag ctcgtggaga tggatctgaa gccgcgggac atcgtgaacc gcgagagcgt
     2281 ggacaacgcc ttctgtctgg acatggccat gggtgggtcc accaacacgg tcctgcacac
     2341 cctggcgatc gcccacgagg ccggcgttcc ctactcgctg gaccggatca accagctctc
     2401 ggaccgggtg ccgtacctct gcaaggtgag cccggcggga caccaccacg tggaggacgt
     2461 gaaccgggcg gggggcatca gcgccatcct gaagcggttg gcagagaagg gggacctcct
     2521 gaggctggag cgccccacgg tgacgggaaa cacgctgggc gaggtcatcc ggcacgcgag
     2581 cgtcaaggac ccggaggtca tccggcccct ggagaatccg taccggcaga caggtgggct
     2641 ggccgtcctg tttggaaacc tggcccccga gggcggcgtc gtcaaggcag ggggcgtggc
     2701 gcccgagatg cttcggcatg aagggccggc ggtgatcttc gagtctcagg acgacgccct
     2761 ggcgggcatc ctcgccggca aggtcaaggc cggggatgtc gtcgtgatcc ggcacgaggg
     2821 gccccgaggc ggacccggca tgcaggagat gctgtcaccc acggccgcca tcatcggccg
     2881 cggcttgggg aactccgtgt ccctcatcac cgacgggcgg ttctcggggg cgacccacgg
     2941 cggctgcatc ggtcacatct cgcccgaggc ggccgcgggc ggccccatcg gcctgctcaa
     3001 ggccggcgat cggatccgta tcgacatcga caagcgcctg ctggaggtgg aactctcgga
     3061 ggcggaactg aaggtgcgcc gcgctgcctg gcggcctccg gagccgcgcg tgaagaccgg
     3121 ctggctgtat cgctattcca ggatggtcac ctcggggagc cagggagcgg tgctgaaggc
     3181 cgattagcgc ggggttcgtg accggaactg ggggctgatt ggacgcgagc gtagggggct
     3241 gaccctcggg acctcgtgga cagtcaatgc gacggttccc ctctccgaca cgagccgggc
     3301 gttctccgga aggtgaatca accgcagggg gcgggcctgt aggcgggttc gccccctggt
     3361 ttttgtgcgg atgaggcggg cgtgatttcc ggctgggttg catgccggac tgccccggtc
     3421 ggtggctgtc aggcccctgc atcaccggct gatcctgcga gggaggcgag gtgcagccca
     3481 gggcagaagc ggcggtgcct gctggcgcga cggtgccagc cgatcggcgg tacatctccc
     3541 ggctcgtgcc actcttctgc gccggctggg ctgtcctcta cgccgatcga accgt
//
LOCUS       DBNGZC010000827         3522 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_266450, whole genome shotgun sequence.
ACCESSION   DBNGZC010000827 DBNGZC010000000
VERSION     DBNGZC010000827.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_266450"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="AC16G0_14220"
     CDS             <1..1072
                     /locus_tag="AC16G0_14220"
                     /inference="COORDINATES: protein motif:HMM:NF037144.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5100805.1"
                     /translation="TLTVVGFGAIDPLTGQLVTPALLAPPANAAVRSSAGGLNQMQVS
                     TGPVAGTGGGSGTPAPVALNDEVQISEDCQPLALGQLGQPHTACIAPAVIINVLSNDT
                     NAVGGTVTLASQPLLGTATVNPDNTISYTPKLDANMVDTFTYTVTVGTQTSNAALITV
                     YILPVNDQPVAVNDSIFAAVNKLVTFNVLANDTDPDGATDLANAVIAGAWPAGLGAQP
                     VPVAGSISFTPTSTGTFSFTYRAQDKAGALSANAATVTVTVSAADNIVVTKSQYVVAK
                     NRWVVSGTDSIPGGQTLSIRYANGSLAGFQIGTAVVDALGNWLFDQVGLTGQFDPRTS
                     GATKLTITSPFGGAATATINLK"
     gene            1402..1845
                     /locus_tag="AC16G0_14225"
     CDS             1402..1845
                     /locus_tag="AC16G0_14225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5100806.1"
                     /translation="MKVLVVDDSAIVGERLASILSEFIEVELLDQVKNGIEAVERIRT
                     LKPDVVILDIGLPGRNGMEILEEVKRQESAPFVVILTNYPYPQYQQRCAEAGADFFFD
                     KSIEFDRVPQTIKQLIHGSSEAGKSHAVVFTGKKKRIDKFGRQEG"
     gene            1848..>3522
                     /locus_tag="AC16G0_14230"
     CDS             1848..>3522
                     /locus_tag="AC16G0_14230"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HIR5100807.1"
                     /translation="MKAKFSPPGRKVAAIVMAVALIGQGFMAGTSQAQVQTISCPTGF
                     GAIDAVVLVRPILLDSLKAVPNPVIPRDPFTGNPTLRADFVDYVANLTAAIQLGKALF
                     WDMQVGSDNKTACATCHHQAGADGRNKNQLNPGANGGWDAFVPNYAFASTDFPFTDPT
                     VPRDVDNVAGSQGVRNSTFVSIKNGAESTTPVSDPVFSVGGANVRQVTGKNTPSAINA
                     VFNHRQFWNGRAQPEFNGVNPFGNRDAGARVWVVGSKGSPVTIDIHIQNASPASQAVG
                     PVLNSVEMSAAGRTFADVGQKLLAAKPLNGQKVSQTDSVLGPLADVTTGLKTTYTALI
                     QQAFQPKWWNSNRSVSVGGKSYSMMAANFSLFWGLSIMLYEATLVSDDSPMDQYLAVV
                     GTARPGPTDPIAAILDQAVNRLASEGITVTRESILDGLNLFERPVAPAPSFPPPPGFG
                     AGCTACHFGGELTSASVRNLTAGGLEPGDVALKNAGFDIRMERMFTKMDWTPPGPLTP
                     VPLGADVITFDPSTYTINVTGISGGPVAPIRLPVAVYDAGWYNLGVRPPA"
BASE COUNT          673 a         1140 c         1096 g          613 t
ORIGIN      
        1 cacgctgacc gtggtaggct tcggggctat cgaccccctc accggccagc tcgtcacccc
       61 ggccctcctg gcaccgccgg ccaacgcggc agtgcggtcc tctgccggcg gcctaaacca
      121 gatgcaggtg agcacgggcc ccgtggccgg gaccggtggc ggcagtggca ccccggcccc
      181 ggtggcgctg aatgacgagg tgcagatctc cgaggactgc cagccgctgg cgctcggtca
      241 gctcggtcag ccacatacgg cctgcatcgc gcccgcggtg atcatcaacg tccttagcaa
      301 tgataccaat gccgtcgggg ggaccgtgac cctggcgagc cagcccctgc ttgggactgc
      361 gacggtcaat cccgataaca cgatcagcta cacgccgaag ctggacgcga acatggtcga
      421 caccttcacc tatacggtga ccgtggggac gcagacctcg aacgccgccc tcatcaccgt
      481 gtatatcctc ccggtgaacg atcagcccgt tgccgtgaac gactcgatct tcgcggccgt
      541 caacaagcta gtcacgttca acgtgctggc caacgacacc gatcccgacg gcgccaccga
      601 tctcgcgaac gccgtgatcg ctggggcgtg gcccgcgggg ctcggcgcac agccagttcc
      661 ggtcgccggt tccatctcct tcacccccac ctcgaccggc accttcagct tcacctaccg
      721 agcccaggac aaggcgggtg ccctgtccgc caacgcggcc acggtgacgg tgaccgtgag
      781 cgccgcggac aacatcgtcg tcaccaagtc ccagtacgtg gtcgccaaga accgctgggt
      841 cgtgtcaggc acggacagca tccctggagg ccagacgctc tcgatccgct atgccaacgg
      901 cagccttgcc ggcttccaga tcgggacggc cgtggtggac gccctcggca actggctctt
      961 cgatcaggtc ggcctgactg gccagttcga tcccaggacc tcgggcgcga caaagttgac
     1021 aatcacctca ccatttggcg gcgcggccac agcgaccatc aacctgaagt agcccgaacc
     1081 ggcctaaagg ccgcgcagga gggagcccgg accgcaaggt ccgggctctt tctatgtcat
     1141 tacgacgaat gaaaaccctg gccagttgtc cgggaggagc aacccccacc cttgccctcc
     1201 ccccgttgcg gggaaaggga cgggtgggag gattttcccc tcccgaatcc ccggattttc
     1261 cccctttcca ccctgggata caccgactta tcggcgctca ctgaggacct tatattgtca
     1321 tcattcaagg aaacagtgcg gcatgtttgc tcttgagaca gagattggtc aaccggggag
     1381 gggcgaaatg gagacgacgc catgaaggtc ctagtcgtgg acgattcggc gatcgtgggg
     1441 gagcgattgg cttcgattct ttccgagttc atagaagttg aacttctgga tcaggtgaag
     1501 aatgggatcg aggctgtgga gcgcatccgg acactgaaac ctgatgtggt gatcctggac
     1561 atcgggttgc ctggacgaaa cgggatggag atcttggagg aggtgaagcg gcaagagtcg
     1621 gctccctttg tcgtgatcct caccaactac ccctatcccc agtatcagca acgctgtgca
     1681 gaggctgggg cggatttctt ctttgacaag tccatcgagt tcgacagggt ccctcagacc
     1741 atcaagcaat tgattcacgg cagttcggag gcggggaaat cccatgcggt tgttttcact
     1801 gggaagaaga agaggatcga taagttcggc cggcaggagg gatgacgatg aaggcgaagt
     1861 tcagtccacc cggaagaaag gttgctgcca tcgtgatggc agttgctctg attggccagg
     1921 gattcatggc tgggacctcg caggcgcagg tgcaaacgat ctcatgcccc acgggattcg
     1981 gggcgatcga tgccgtggtg ctcgttcgtc cgatcctgct cgactcgctc aaggcggtgc
     2041 caaatccagt tatccccagg gacccgttca ccggcaaccc gacgctgaga gcggatttcg
     2101 ttgactatgt cgcgaatctc acggctgcca tccagctcgg caaggcactc ttctgggaca
     2161 tgcaggtggg gagcgacaac aagacggcgt gcgccacctg ccaccaccag gccggcgctg
     2221 acgggcggaa caagaaccag ctcaatccag gtgccaacgg gggttgggat gcgtttgtcc
     2281 ccaactacgc cttcgcgtca acggatttcc cgttcacgga tccgaccgtc ccaagagacg
     2341 ttgacaacgt cgctggctcg cagggcgtcc ggaacagcac ctttgtcagc atcaagaacg
     2401 gggcggagtc gacgacccct gtgtccgatc cggtgttcag cgtcggcggc gcgaatgtcc
     2461 ggcaagtgac ggggaagaac accccatcgg cgatcaacgc ggtgttcaac caccgccagt
     2521 tctggaacgg gcgcgcccag ccggagttca atggcgtgaa cccgttcggc aaccgggacg
     2581 ccggcgcccg cgtctgggtc gtgggtagca aggggagccc ggtcaccatc gacattcaca
     2641 tccagaatgc aagcccggcg tcccaggcgg tagggcccgt gctcaactct gtcgagatgt
     2701 cggcggcggg ccggacgttc gctgacgtcg gccagaagct gctcgccgcc aagccgctga
     2761 acggccagaa ggtgagccag accgacagcg tcttgggccc cctggccgac gtgacgacag
     2821 gactcaagac gacgtacacg gcgctgatcc agcaagcctt tcaaccgaag tggtggaaca
     2881 gcaacaggtc ggtcagcgtc ggcggaaagt cgtactccat gatggcggcg aacttctccc
     2941 tcttctgggg cctctcgatc atgctctacg aggccaccct ggtctcggat gatagcccca
     3001 tggaccagta cctggctgtc gtcggcaccg cccgtccggg gccgaccgat cccatcgccg
     3061 ccatcctgga ccaggcggtg aaccggctgg catccgaagg catcacggtc acgcgcgaga
     3121 gcatcctgga cgggctcaac ctcttcgaac gacccgtggc tcctgcgccc tccttcccgc
     3181 cgccgcctgg cttcggggct gggtgcacgg cgtgccactt cggcggtgag ctgacgagcg
     3241 cctccgtgcg gaacttgacg gcaggtgggc tcgagcctgg agacgtggcc ctcaagaacg
     3301 ccggcttcga cattcgaatg gagcgaatgt tcacaaagat ggactggacg cccccggggc
     3361 cgctgactcc tgtgccgctt ggggccgacg tgatcacgtt tgacccgagc acctacacca
     3421 tcaacgtgac tgggataagc ggtggcccgg tcgccccgat ccggctgccc gtcgccgtct
     3481 acgatgccgg ctggtacaac ctcggcgtgc gcccccccgc gg
//
LOCUS       DBNGZC010000828         2986 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_558973, whole genome shotgun sequence.
ACCESSION   DBNGZC010000828 DBNGZC010000000
VERSION     DBNGZC010000828.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_558973"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="AC16G0_14235"
     CDS             <1..653
                     /locus_tag="AC16G0_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100808.1"
                     /translation="IDEADTIWPNSSGQITQQPLSAYVRRLGANLQVGVWPTPAATDP
                     STVLIGGVSDTSTAPMILADVTNFLSFGYVQIDAEIIAYQFLVSALPGISVITRGIGG
                     TTPASHADGATVQHLGFWIKGRRIPNPITNSLSCVEVPRAWMSHLETYVLAQVRMEQK
                     GRFREGQALLQSFERACASINADPNWKVNRGLAHAFGDPLTIGGIYWPRSGGVIVP"
     gene            663..1574
                     /locus_tag="AC16G0_14240"
     CDS             663..1574
                     /locus_tag="AC16G0_14240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100809.1"
                     /translation="MAYLAGTIINNVRTLIPEQIFLAGNPQPDTDGNIRAATLYSWLD
                     DGVRLMTNQLGWTLVDWSAVAAVTGQAIYPINSLFQLVDQAYANQIKCRLLSQPDADS
                     IYPGRGTEAQANTAYLYRVTDHIEIGFEPVPSASDPSTTLNGGINASVTSITLTSAAG
                     FLPYGYALIESELVQYQLIAGNVLQLCTRGAGGTAAVAHLTGAAVTHCSLWIKGSRTP
                     STITSSTSPVEVPLGWVSLLNTYLLSMCRQSQGNFPVAQQLLDSFNKMVETIAKQFAG
                     KDPLTMRAALESPVSTGVNFDMREQTG"
     gene            1578..2489
                     /locus_tag="AC16G0_14245"
     CDS             1578..2489
                     /locus_tag="AC16G0_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100810.1"
                     /translation="MAYLAGTIINNVRDLIPDPVYSLSVPQPDADGGLFRAQTLYRWL
                     DDGVRLMTSAAGYNLTDWTAVAAIANQPVYPIDPLFLVIDQAYSSQLRCRLINQTLMD
                     AIYPGRGTQSKARTAYVNRVTGQVELGFQPVPSAADPATTLNGGISATDTSIVLASTA
                     GFLSYGFVLIESELVRYQSISANTLNQCTRGMGGTAAVAHLTGVAATHCSLWVKGSRS
                     PMTITDSTSVVEVPLAWVSLLNLYLLGMCRNSENEFGEGGRLLKEFDAAVAGIAKGFA
                     GRDVMTPQAARQSRATTGVDTKPPGGA"
     gene            2503..>2986
                     /locus_tag="AC16G0_14250"
     CDS             2503..>2986
                     /locus_tag="AC16G0_14250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100811.1"
                     /translation="MKARWLALVLIVLLPQTASSQPSPAPVIATGILQQGTQGAVNGH
                     VLNTPQLAVMVFQVSGTFTASIAFEGSLDGTNWQRLECYDFTGVLVSTDVSSAGAWRC
                     NINGILQARARITTYMNGAVTVIGSATSTPLVFKNQTGIGPPGPTGPAGASGPAGPTG
                     P"
BASE COUNT          532 a          975 c          962 g          517 t
ORIGIN      
        1 tgatcgacga ggcggacacc atctggccga acagctcagg gcagatcacg cagcaaccac
       61 tgtccgccta tgtgcggcga ctcggggcca atctccaggt cggggtctgg ccgacgccgg
      121 ccgcgaccga cccgtccacc gtcctgatcg gcggggtgag cgataccagc accgccccca
      181 tgatcctcgc ggacgtgacc aattttctgt cgttcgggta tgtgcagatt gatgcggaga
      241 tcatcgccta tcagttcctc gtctcggccc tgccgggcat ctccgtcatc acccgcggca
      301 tcggcggcac gacgcctgcg agccatgccg acggggccac cgtccagcac ctcggcttct
      361 ggatcaaggg ccggcggatt ccgaacccga tcaccaatag cctctcgtgc gtggaggtgc
      421 cccgcgcctg gatgagccat ctggagacct acgtgctggc ccaggtcagg atggagcaga
      481 aggggcgctt ccgggagggg caggcgttac tgcagagctt cgagcgtgcc tgcgcgagta
      541 tcaacgccga cccgaactgg aaggtgaacc ggggattggc ccatgccttc ggcgatcccc
      601 tgaccatcgg cgggatctac tggccgcgat cgggcggcgt catcgtcccc tagaggagcg
      661 ccatggccta tctcgccggc accatcatca acaatgttcg caccctcatc ccggagcaaa
      721 tcttcctggc cgggaacccg cagccggaca cggacggcaa tatccgcgcc gcgaccctct
      781 attcctggct cgacgatggc gtcaggctga tgaccaacca gctcggctgg acgctggtgg
      841 actggagcgc ggtggcggcg gtgacgggac aggccatcta tcccatcaac agtctctttc
      901 aactcgtgga ccaggcgtat gccaatcaga tcaagtgccg tctcctgagc cagccggatg
      961 cggacagcat ttatcccggg cgcggcaccg aggcccaggc caacaccgcc tatctctatc
     1021 gcgtgacgga ccacatcgag atcggcttcg agcccgtgcc gagtgccagc gacccgtcca
     1081 cgacgctcaa cggcgggatc aacgcgagcg tgacgagcat caccctgacc tcagcggcgg
     1141 gcttcctgcc ctacgggtat gccctgattg agtcggaatt ggtgcagtac cagctcatcg
     1201 cgggcaacgt gctccagtta tgtacccgag gggccggcgg gacggcggcc gtcgcgcacc
     1261 tgaccggcgc ggccgtgacg cattgtagcc tctggatcaa ggggtcacga actccgagca
     1321 ccatcacgag cagcacctcg ccggtcgagg tgcctttggg ctgggtctcc ttgctcaaca
     1381 cctacctgct gtcgatgtgc cggcagagtc aaggcaattt cccggtggcg caacaactcc
     1441 tcgactcgtt caacaagatg gtcgagacga ttgccaaaca gttcgcgggg aaggatcccc
     1501 tgacgatgcg ggcggcgctg gaaagcccgg tcagtaccgg cgtgaacttc gatatgcggg
     1561 agcagacagg ataagccatg gcctatctcg ccgggaccat catcaacaat gtgcgcgacc
     1621 tgatccctga tccggtctac agcctcagtg tcccgcagcc ggacgccgac ggcggcctct
     1681 tccgggcgca gacgctctat cgatggctcg acgatggggt ccggctcatg acctcggcgg
     1741 cagggtacaa cctgacggac tggacggcgg tggcggcgat tgccaatcag ccggtgtatc
     1801 ccatcgaccc gctctttctg gtgatcgacc aggcgtattc gagccagttg cgctgtcgcc
     1861 tcatcaacca gaccttgatg gacgcgatct acccaggtcg cggcacccag agcaaggcgc
     1921 ggaccgccta cgtcaaccgc gtcacggggc aggtcgaatt ggggttccag ccggtcccga
     1981 gcgcggcgga cccggcgacg actctcaacg gcgggatttc ggcgaccgac acctccatcg
     2041 tcctcgcgtc cacagccggg tttctttcct atggcttcgt cctgatcgag tctgaactgg
     2101 tccgctatca gtccatctca gcgaatacgt tgaaccaatg cactcgtggg atgggcggga
     2161 cggcggcggt ggcgcatctg accggagtcg cggcgaccca ctgtagcctc tgggtcaagg
     2221 gctcgcggag ccccatgacg atcacggaca gcacctcagt cgtggaggtg cctttggcct
     2281 gggtgtcgct cctgaatctc tatctcctcg gcatgtgccg gaactcggag aacgagttcg
     2341 gggaaggcgg acggctgctg aaggagttcg atgccgcggt ggcggggatc gccaaggggt
     2401 ttgccgggcg ggatgtcatg acgccgcagg cggcacggca gagtcgggcc accaccggag
     2461 tcgataccaa gcctccgggt ggggcatgag aaaggaactc cgatgaaggc tcgatggctg
     2521 gcactggtcc tgatcgtgct cctgccgcag acggcctcgt cccagccctc tccggcgccc
     2581 gtgatcgcca cggggattct gcagcagggg acgcagggcg cggtgaatgg gcacgtcctc
     2641 aacacgccgc aactggctgt catggtcttt caggtctcgg gcaccttcac ggcctcgatt
     2701 gccttcgagg ggtcgctgga tggcacgaac tggcagcggc tagagtgcta cgacttcacg
     2761 ggagtcctgg tctcgactga cgtgtcctcg gccggtgcct ggcggtgcaa catcaacggc
     2821 atcctccagg cgcgagctag gatcacgacc tacatgaacg gggcggtgac ggtgatcggg
     2881 agcgcgacct cgacgcctct agtcttcaag aaccagacgg gcattggccc acctgggccc
     2941 accggcccgg ccggtgcgtc gggtccggcg ggccccaccg gaccga
//
LOCUS       DBNGZC010000829         2116 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2324169, whole genome shotgun sequence.
ACCESSION   DBNGZC010000829 DBNGZC010000000
VERSION     DBNGZC010000829.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2324169"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="AC16G0_14255"
     CDS             complement(<1..394)
                     /locus_tag="AC16G0_14255"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014663.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit C family protein"
                     /protein_id="HIR5100812.1"
                     /translation="MSELRPNGSRLGFEVLRPGLLTTVQDRGRTGYQRFGVPVSGAVD
                     QAALRVGNVLVGNPQDTAALEIALQGPQLRLLTDVVLALTGAEFAATLDAEPLPSCES
                     FAAHPGQVLDIQMAVSGLRAYLSVAGGFD"
     gene            complement(391..1107)
                     /gene="pxpB"
                     /locus_tag="AC16G0_14260"
     CDS             complement(391..1107)
                     /gene="pxpB"
                     /locus_tag="AC16G0_14260"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HIR5100813.1"
                     /translation="MRVRFLPAGDRGLVVEFGNEVSSAISAQVRTLDADLAAARIPGV
                     VEAVPTYRSLGIEYDPSEISFEALQAHVREVLAKLDPGVLPPPRRVRIPTVYGGEYGP
                     DLPFVAEHACLSQAEVIRLHSQTVYHVYMIGFAAGFAYLGGLPERLHTPRLPSPRIRV
                     PKGSVGIGGNQTGAYPAETPGGWRLIGRTYLELFDPSQEIPTPMVPGDEVEFLPVTEA
                     EFLAASREPRVASHETRGTE"
     gene            complement(1164..1931)
                     /locus_tag="AC16G0_14265"
     CDS             complement(1164..1931)
                     /locus_tag="AC16G0_14265"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010885076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HIR5100814.1"
                     /translation="MQIDLNSDMGESYGAYTIGADEEILRWVTSANAACGWHGGDPNV
                     MRRTVQKAKELGVRVGAHPSYPDLLGFGRRVMQISRQEARDYVLYQIGALRAFADALE
                     VKLQHVKPHGAIYNAAAKDRELSLGIAEGVADAGGDLVLVGLPGSEIVKAGREVGLRV
                     AREAFGDRAYNEDGSLVSRKIAGSLITDPDVVAERVLGFVRGSVTAISGREIKVEADT
                     ICLHGDTPGAGAIARRVRECLEAAGVKLTPMGDFVRR"
BASE COUNT          338 a          762 c          661 g          355 t
ORIGIN      
        1 cgtcgaagcc gcccgccacg ctgaggtagg cccgcaggcc gctgacggcc atctgaatgt
       61 ccagcacctg gcccggatgt gccgcgaagg attcgcagga cgggaggggc tctgcgtcca
      121 gcgtggccgc gaactccgcc ccggtcagcg ccaagacgac gtcggtcagc aggcggagct
      181 gcggcccttg cagcgcgatc tccaaggccg ccgtatcctg tggattccct accaggacgt
      241 ttcccacgcg cagcgcagct tggtccacgg caccgctcac agggaccccg aaacgctggt
      301 acccggttct cccccgatcc tgaaccgtgg tcaagagccc cggccggagc acctcgaatc
      361 cgagccgcga gccgtttggc cggagttcgc tcattccgtt cctctcgtct cgtgactcgc
      421 gactcgcggc tcgcggctcg ctgccaggaa ttctgcctcg gtgacgggca ggaactccac
      481 ctcatccccc ggcaccatcg gcgtggggat ctcctgcgac ggatcgaaga gctcgaggta
      541 cgtgcggccg atgagccgcc agccgccggg ggtctcggcc ggataggcgc cggtctgatt
      601 gccaccgatg cccacggagc ccttgggaac tctgatccgg ggcgagggaa ggcggggcgt
      661 gtgcagccgc tctggcagcc cgccgaggta cgcaaaccca gccgcaaagc cgatcatgta
      721 gacgtgatac accgtctgac tgtgcaggcg gatcacctcc gcctgggaga ggcaggcatg
      781 ctcggccacg aagggcagat cgggcccgta ctctcctccg tagaccgtcg ggatgcgtac
      841 ccgcctgggg ggaggcagga cgccggggtc gagtttcgcc agcacctcgc ggacgtgcgc
      901 ctgcagtgcc tcgaaagaga tttcggacgg atcgtactcg atcccgaggg atcggtaggt
      961 gggcaccgcc tcaaccaccc cggggatccg agcggccgcg agatctgcat ccagggtgcg
     1021 aacttgggca ctgatggccg aactgacctc gttcccgaac tcgaccacca agccccgatc
     1081 ccccgccggg agaaagcgga ccctcatcgc ttcaccctgt cgctgttccc gtcccagggg
     1141 ctcttccgcc tccgccccgg gaatcatcgc cggacgaagt cgcccatggg cgtgagcttg
     1201 accccggccg cctcgagaca ttccctgaca cggcgggcga tcgcccccgc acccggggtg
     1261 tccccgtgga gacagatcgt gtccgcctcg accttgatct ccctcccgct gatggcggtc
     1321 acgctgccgc ggacgaagcc gaggacccgc tcggccacta cgtccgggtc ggtgatcagg
     1381 gatccggcta tcttccgcga gaccaggctg ccgtcctcgt tgtaggcccg gtcgccgaag
     1441 gcctcgcgcg ccacgcgtaa cccaacctct cgtcccgcct tcacgatctc cgagccgggg
     1501 aggcccacca ggacgagatc cccgccggcg tcggccaccc cttcggcgat gcccagggac
     1561 agctcccgat ccttggcggc ggcattgtaa atcgctccgt ggggcttcac gtgctggagc
     1621 ttcacctcca gggcgtcggc gaacgcccgc agggccccga tctgatagag cacgtagtcc
     1681 ctggcctctt gccggctgat ctgcatgacc cgtcggccga agcccaagag gtcaggatag
     1741 ctcgggtgtg cccccacgcg cacgcccagc tctttcgcct tttgcaccgt gcgccgcatt
     1801 acgttcgggt cgcctccgtg ccagccgcag gccgcgttgg ccgaggtcac ccagcgcagg
     1861 atctcctcat ccgcgccgat ggtgtaggca ccgtacgact ctcccatgtc gctgttcagg
     1921 tcgatctgca tgctcggccc cttccctcat ttcgtggatt ggcaagcgac tgaagaagcg
     1981 attcgcgccc aggttgctca cgagagtcca gaggcaagac agcgtcatcc atcctccgga
     2041 tgggtacccg gcagatgggt ccttttcacc ggcttcctat atgccgagat agacctctcg
     2101 aacccgcttc gactcc
//
LOCUS       DBNGZC010000830         3570 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_657525, whole genome shotgun sequence.
ACCESSION   DBNGZC010000830 DBNGZC010000000
VERSION     DBNGZC010000830.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_657525"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1097
                     /locus_tag="AC16G0_14270"
     CDS             270..1097
                     /locus_tag="AC16G0_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100815.1"
                     /translation="MTTEDIMEVALDLAQLHDVPADSGVHVRSDSVKKLLATIDCDVA
                     DLLLARELKCDAVLTHHPEGRASLHGWKLIRRQIEQMVECGVPVAKAEAAVQRRMQTV
                     EVNSHTRNYGRVVQAAQLLKLPFLNVHLPCDIITRRLIAEKMAPFNRPESRASVSDVV
                     TALQEFPEQKLAATEPQVRLGAPDRLAGRVAVAMAGYTNGGVDVLRAYFEAGVGTVLM
                     MHFPENELREAREQKLAGNLVITGHMASDSIGINLYLDELERLGLKVTRAGGIITAH"
     gene            complement(1218..1451)
                     /locus_tag="AC16G0_14275"
     CDS             complement(1218..1451)
                     /locus_tag="AC16G0_14275"
                     /inference="COORDINATES: protein motif:HMM:NF013224.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR5100816.1"
                     /translation="MPVLAFIFLECTVGKAKEVATKVAKIAGVRISHAVTGAYDVIAL
                     IEAPDVNALGTTVVSKIQSVPGVLRTSTNVVVE"
     gene            complement(1504..1704)
                     /locus_tag="AC16G0_14280"
     CDS             complement(1504..1704)
                     /locus_tag="AC16G0_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100817.1"
                     /translation="MSGGLVDCPLCQGLKKIGCPTCGGSGVLRTRQEGGDILSSCKAC
                     HGIGVCQCPACQGSGLKQVLPF"
     gene            1969..3354
                     /locus_tag="AC16G0_14285"
     CDS             1969..3354
                     /locus_tag="AC16G0_14285"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014854932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR5100818.1"
                     /translation="MHPAIGYIQEQREAFVEELKAFLRIPSVSTKSEHKGDIQKAAEW
                     LAGEMRAIGLEHAQIMPTLGHPVVYADWLHAPGKPTVLIYGHYDVQPAEPLDLWISGP
                     FDPTVRNGELFARGAVDDKGQVFMHLKALQAHLTTSGKLPVNVKLLIEGEEEIGSPNL
                     DALILAQQALLRADAVVISDTAMIAKGSPGITHGLRGLIYFQIDVEGTKSDLHSGSFG
                     GAVINPGFALAQILSQLKDRNGKVTIPHFYDDVRKLAIEERRALARLPFSEKKFQKEI
                     GAPALFGEKGFTTLERLWVRPTLEINGLYSGFIGEGAKTVLPGRAMAKVSMRLVPDQN
                     PAKIARLFSQHVKRLSPKTIRVKVTEISGRGAPWLTPTDHPAMQAMARAIQQGFGKKP
                     VYTRTGGTIPVVATLTRLLKAPILLMGIGLPDENAHAPNEKLDLDHLHHGMLAAAHLW
                     NELADTKLTKA"
BASE COUNT          707 a         1154 c         1091 g          618 t
ORIGIN      
        1 ccagatacag gagtgtctgg agatagatac gcctggatgc cgtagacccc ctctgtcgac
       61 gcccacgaag gggaagaagt gcccctgcgc cccgcgcgct tcccggtggg actattgacg
      121 gttgccaggt tgagggaggg gatatacccg aatacccgaa tggcacttga ccgatgccgc
      181 caggcgtgtt agacgggaca agctatcttg gatgcaggta gagatggcaa ggcggcaaca
      241 gggccggcga atgcacggga ggagagacca tgaccacgga agacatcatg gaggtcgccc
      301 tggatttggc gcagttgcac gacgttcccg ctgactcagg ggtgcacgta cggagtgaca
      361 gcgtcaagaa gctgttggcc actatcgact gcgacgtggc ggacctgttg ctggcgcggg
      421 aactcaagtg cgacgccgtg ctcacgcatc atccggaagg gagagcctcc ctccacggct
      481 ggaagctaat ccgacgccag atcgagcaga tggtcgagtg cggggtgccg gtggccaagg
      541 ccgaggccgc cgttcagcgg cgcatgcaga ccgtcgaggt gaacagccac acccggaatt
      601 acggtcgggt ggtgcaggcc gcgcaactcc tgaagctccc cttcctcaac gtgcacctcc
      661 cctgcgacat catcacgagg cgtctcatcg cggagaagat ggccccgttc aatcgcccgg
      721 agagtcgggc cagcgtgtcc gacgtcgtca cagccctcca ggagttcccg gagcagaagc
      781 tggcggccac cgaaccgcag gtccgcctag gcgccccgga ccgtctcgct gggcgggtgg
      841 ctgtcgcgat ggctggttac accaacggcg gggttgacgt cctccgggcc tacttcgagg
      901 ccggggtcgg gaccgtcctc atgatgcact tccccgagaa cgagctgagg gaagcacggg
      961 agcagaagtt ggcaggaaat ttggtcatca ccggccacat ggccagcgat tccatcggaa
     1021 tcaatttgta tctcgacgag ctggagcgtc tggggttgaa agtgacccgg gccggaggca
     1081 tcatcaccgc gcactagacg cgcgtccgaa ccatagggca accgccggtg gcaccaacgg
     1141 gcctcgatgc cggcgaatgt gcgtccggga ggacggcgag aagtctcgat ccggggaccg
     1201 ccaggacggg aagctcctca ctccaccacg acgttcgtgg atgtccgcag aacacccggg
     1261 accgactgga tctttgagac caccgtggtt cccagggcgt tcacgtccgg cgcctcgatc
     1321 agggcgatca cgtcgtaggc gccggtcacg gcgtgtgaga tgcggacgcc cgcgatcttg
     1381 gccaccttgg tcgccacctc ttttgctttc ccgaccgtac attcgaggaa gatgaacgcg
     1441 agcacgggca tgttcggtaa ctccattctc gccatcggaa ttgcggagtt caatttgccc
     1501 tgatcagaac ggcaagacct gcttgagccc gctcccctga catgccgggc attgacacac
     1561 accaatcccg tggcaggcct tgcaggacga caggatatcg ccgccctcct gtctggtccg
     1621 caggacccct gacccgccgc aggtgggaca gccgatcttc ttcagcccct ggcacaaggg
     1681 gcagtccacc aatccgccag acattttcca atcctccatg tacactgcga accggctgca
     1741 gcggtcgagc ccgaggctct gtcctcggga ctggaatgca ttctatacca tcagaacagg
     1801 gccacctagt gcaaatcggc acccatatcc tgcccttcgc atttcatcga acaggccggt
     1861 tccgatcgtc ccccaccacc tttcattttg acctgggaag ggtgttgcga tacagtctct
     1921 cgcggcggcg gtcctgctcc tttccccatc ctttccttgg aggattccat gcacccagcc
     1981 atcggctaca tccaagagca gcgcgaggcg ttcgtggagg aactgaaggc cttcctccgc
     2041 atccccagcg tcagcaccaa gtccgagcac aagggcgaca tccagaaggc cgccgagtgg
     2101 ctcgccggag agatgcgagc gatcggcctg gaacacgccc agatcatgcc cacgctcggg
     2161 cacccggtcg tctatgccga ttggctgcat gcccccggca agcccacggt gctcatatac
     2221 ggccactacg atgtccagcc ggctgagccc ctggacctct ggatctccgg ccccttcgat
     2281 cccaccgtga gaaacggcga gctttttgcc cggggggccg tggatgacaa ggggcaggtc
     2341 ttcatgcacc tcaaggccct ccaggcgcac ctcactacga gcggcaagct tcccgtcaac
     2401 gtcaagctcc tgatcgaggg ggaggaggaa atcggaagcc ccaacttgga tgccctcatc
     2461 ctggcgcagc aggctctcct tcgggcggac gcggtcgtga tctcagacac cgccatgatt
     2521 gccaaggggt ccccgggcat tacccacggt cttcgcggcc tgatttattt ccagatcgac
     2581 gtggagggaa ccaagagcga tctccactcc ggcagcttcg gcggcgccgt gatcaatcct
     2641 ggcttcgccc tggcccagat tctgtcgcag ctcaaggatc ggaatgggaa ggtcacgatc
     2701 ccgcactttt acgatgacgt gcgcaagctc gccatcgagg agcgccgggc gctggcacga
     2761 ctccccttca gcgagaagaa attccagaag gagattgggg cgccggccct ctttggcgag
     2821 aagggcttca ccacgctgga gcgactctgg gtccggccga ccctggagat caacggtctc
     2881 tacagcggct tcatcggcga gggcgccaaa actgtcctgc caggtcgggc catggcgaag
     2941 gtgagcatgc ggctggtccc ggatcaaaat ccggcgaaga tcgcccggct cttcagccag
     3001 cacgtgaagc ggctctcgcc caagaccatc cgggtgaagg tgacggagat ctccggccgg
     3061 ggcgccccct ggctgacccc cacggatcat cccgccatgc aggccatggc gcgggccatt
     3121 caacagggat ttggaaagaa gcccgtgtac acccgcaccg gcggcacgat ccccgtcgtg
     3181 gcgaccctga ccaggcttct caaggccccg atcctgctca tgggcattgg cctgccggac
     3241 gaaaatgccc atgccccgaa cgagaagcta gacctggacc acctccacca cgggatgctg
     3301 gccgccgccc atctctggaa cgagctggca gacacgaagc tgacgaaagc ttgactgctg
     3361 ccccgaagaa tgcgacggcc cggaggggtg cgtccacccc cctccgggcc ttttggatat
     3421 ctgctcatcc acttgtgaga tatcgtggac catcaccgtg agtaatcttt ccacgaccca
     3481 gtgctctgct agccattcgt agatcgcccg agcaacctgc gtgttctttc agaccaagat
     3541 gaagaccggc aagggtgaga gcggaagatg
//
LOCUS       DBNGZC010000831         3260 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2324287, whole genome shotgun sequence.
ACCESSION   DBNGZC010000831 DBNGZC010000000
VERSION     DBNGZC010000831.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2324287"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..109
                     /locus_tag="AC16G0_14290"
     CDS             <1..109
                     /locus_tag="AC16G0_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007160194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5100819.1"
                     /translation="ATPNDVAELAAFLASEPAEYITGQAINLGGGLIMH"
     gene            433..>3260
                     /locus_tag="AC16G0_14295"
     CDS             433..>3260
                     /locus_tag="AC16G0_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011020601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR5100820.1"
                     /translation="MNIKPKIGLSLWLVLLVVTAVVPLLLFAGATLFQISKDSLIARD
                     QGQADTARALALAVDGEVRSWKAALTALAGSRTLRDGRLADFYEEALGVAAPHGGWIV
                     LTVSSGQQLINTLRPYGTPLPKTSSPETINAIFASGKPLVSDVFYGKVAQGYLVAVAV
                     PVVRAGKVVNCLTLNFYPQRLTRLLEGQQLPPSWVAAITDRQHQVIARSSDIEKRVGK
                     PVMPWLGAALTAADHGIVSGPLSDGRAGQVAFQRLQEVPWVVALAVPLAELPSSQPLV
                     LFLVGGVVVGVGAVAAALYTGRRITAPVSRLAQASERLLRGEAGDLGPPSGICEVREL
                     QQALGEASAAVQGYYQERERAAVAVETARAASAAEQALRASEERLKLFIQNAPAAIAM
                     FDRDMRYLAVSRRWLSEYGLEDRDLAGKSYYEVFPDIPDAWKIAHRRGLAGEVVQAAE
                     DRFERADGSVQWLRWEGRPWHARDGEIGGLILYTEDITERKQAEVSLQRLAAIVESSD
                     DAIIGKDLAGTMVTWNAGAERLYGYAAAEVLGQHISILVPPDGQDELPPLFQRVLRGE
                     RVTHFETKRLTKDGARLDVSLSLSPIKDRTGAIVGISAIARDITEWKRMEEALRVSEQ
                     RVRLKLDAILSPEGDLGNLELADILDVQAIQSLLENFYNLARMPVSVVDLQGRVLVGV
                     GTQEICTKFHRVHQEACQHCQESDTRLSAGVPAGEFRLYKCKNNMWDVATPIMVGGHH
                     VGNLLSGQFLFEDEPAEQELFRAQARRYGFPEDEYLQALEGVPRMSRESLGTGMAFFM
                     KFAHMLSQLSYSNLKLARSLAERDTLMQSLRTSEEQLRRQREWLRVTLTSIGDAVLTT
                     DMAGRITFLNPVAERLTGWRQDDALGRPVLSVFRTVDERTHEPTEDIVTRALREGCTV
                     LLTNHTTLVARDGREWPIEDSAAPITDNV"
BASE COUNT          581 a         1029 c         1088 g          562 t
ORIGIN      
        1 ggcgaccccg aacgatgtgg ccgaactcgc cgcattcctc gcctcggaac ctgccgagta
       61 catcacggga caggccatca atctcggagg tggcctcatc atgcactaat gaccgggagg
      121 gtttgtaggg gaatcctgtc atttggtctg acataccatc tcatctccac cctttgaacc
      181 tctcggctgc catagtacat tgcccttggc cgcatcccca tcactgcagc gaccggccag
      241 tagttggagt gcccggatta tccgcgtagc cgttaacccg tgtctgcttc cttgtgattt
      301 tcttccgtag ggagggtccc gagaccgccc tggcaatctt ccagacaggc aggccgacgg
      361 tgaccattga gttgggctga tcctcgcccg cagccggctt ggcgttgggg cttgcacgct
      421 ggggaggaaa gcgtgaacat caaaccgaag attggtttga gtctctggct cgtcctgctc
      481 gtcgtcaccg ccgtggtgcc gctcttgctc ttcgccgggg ccaccctctt ccagatctcg
      541 aaggactcac tgatcgccag agatcagggg caggccgata cagcccgagc ccttgccctg
      601 gcggtggatg gggaggtgcg gtcatggaag gccgccctca cggcgctggc cggatcgaga
      661 accctgcggg acggtcgctt ggcggacttc tacgaggaag ccctcggggt cgccgcgccg
      721 cacgggggct ggatcgtgct cacggtgtca tcggggcaac agctcatcaa caccctccgt
      781 ccctatggca ccccgctgcc caagaccagc tcgcccgaaa ccatcaacgc catcttcgcg
      841 agtggaaagc cgctggtgag cgatgtgttc tacgggaagg tcgcccaagg ctatctcgtg
      901 gccgtggcgg tgcccgtggt gcgtgcgggg aaggtggtca actgcctcac gttgaatttc
      961 tatccgcagc gtctgacccg gctcctcgaa ggccagcaac ttcccccttc ctgggtcgcc
     1021 gccatcaccg accgccagca ccaggtaatc gcccgatcca gcgatatcga gaagcgtgtg
     1081 gggaagccgg tgatgccatg gcttggagcg gccttgaccg ccgcggacca cgggatcgtc
     1141 agtggacccc tcagcgacgg ccgggcgggg caggtggcct ttcaacggct ccaggaagtg
     1201 ccgtgggtgg tggccctggc ggtgcccctg gccgagctgc cgtccagcca acccctggtg
     1261 ctcttcctcg tcgggggggt ggtggtgggc gtgggcgccg tggctgctgc tctgtacacc
     1321 ggacgcagga tcacggcacc cgtatcccgg ctggcccagg ccagcgagcg gctgctccgg
     1381 ggggaggcgg gcgacctggg tcccccgtct ggcatttgcg aggtgcgaga gctgcaacag
     1441 gcgttggggg aggcctcggc cgccgtgcag gggtactacc aggagcgcga gcgggccgcg
     1501 gtcgccgtgg aaacggccag ggcggcctcg gctgccgagc aagccctgcg ggcaagcgag
     1561 gagcggctca agctcttcat ccagaatgcg ccggccgcca tcgccatgtt cgaccgggac
     1621 atgcgctacc tcgccgtgag ccgccgctgg ctgagcgagt acggtctgga ggatcgggac
     1681 ctcgccggca aatcctacta tgaggttttt cccgacatcc cggacgcctg gaagatcgcg
     1741 caccgacgtg gtctggctgg cgaggtcgtg caggccgcgg aggaccggtt cgagcgggcg
     1801 gatgggtcgg tgcagtggct ccggtgggag gggcggccgt ggcatgcacg ggacggcgaa
     1861 atcggcggcc tcatcctcta caccgaggac atcacggagc ggaagcaggc ggaggtgagt
     1921 ctccagcgac tggcggccat cgtggaatcc tcggacgacg ccatcatcgg gaaagacctc
     1981 gccgggacaa tggtcacctg gaacgcgggg gcagagcggc tctacggcta cgctgcggcg
     2041 gaggtcctgg gacagcacat ctcgatcctc gtcccaccag acggacaaga cgagttgccc
     2101 cccctcttcc agcgggtctt acgcggggag agggtcaccc acttcgagac gaagcggctc
     2161 accaaggacg gtgctcggct ggatgtctcg ctcagcctgt ctcccatcaa ggataggacg
     2221 ggggcgatcg tcgggatatc cgccatcgcc cgggacatca ccgaatggaa gcggatggaa
     2281 gaggcgctgc gggtcagcga gcaacgtgtc cggctgaaat tggacgccat tctttcgcct
     2341 gaaggcgacc tggggaacct ggagctggcc gacatcctgg atgtccaggc gatccaatcg
     2401 ctcctggaga acttctataa cttggcgcgc atgcccgtga gtgtcgtcga cctgcaaggc
     2461 agggtgctgg tcggcgtggg cacgcaagag atctgcacga aatttcaccg ggtgcaccag
     2521 gaggcttgcc agcattgcca ggaaagcgac acgcggctct cagccggggt ccctgcggga
     2581 gagttcagac tctacaagtg caagaacaac atgtgggatg tcgccacccc gatcatggtc
     2641 gggggccacc acgttggcaa tctcctctcg ggccaattcc tattcgaaga cgagccggcg
     2701 gagcaggaac tcttccgcgc tcaggccagg cgctacggat tccccgagga cgagtacctc
     2761 caggcgctgg aaggcgtccc acgcatgagc cgggaatccc tgggcaccgg gatggcattc
     2821 ttcatgaagt tcgcccacat gctttcccag ttgagttaca gcaatcttaa gctggcacgc
     2881 tccttggcgg aacgcgacac cttgatgcag tccctgcgga cgagtgagga gcagctccgg
     2941 cgacaaaggg agtggctgcg ggtcacgctc accagcatcg gcgacgcggt gctgaccacc
     3001 gacatggccg gccggatcac gttcctcaac ccagtcgccg aaaggctgac cggctggcga
     3061 caggacgacg cgctgggccg gccggtgctg agcgtgttcc gcactgtgga cgaacgcacc
     3121 cacgagccca ccgaggacat tgtgacgcga gcgctgcgtg agggctgcac cgtcctgctg
     3181 accaaccaca ccacactggt tgctcgcgac ggtcgagaat ggcctattga agacagcgcc
     3241 gcgccaatca cagataatgt
//
LOCUS       DBNGZC010000832         6219 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_755908, whole genome shotgun sequence.
ACCESSION   DBNGZC010000832 DBNGZC010000000
VERSION     DBNGZC010000832.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6219)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6219
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_755908"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..714)
                     /gene="rimI"
                     /locus_tag="AC16G0_14300"
     CDS             complement(208..714)
                     /gene="rimI"
                     /locus_tag="AC16G0_14300"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR5100821.1"
                     /translation="MRPAPPSALRIRPMTARDLGRVVAIEAVCFGKEAWPFQTFKELL
                     AAFSKAEPTRGEMWVAEDPDTGELLGYAGVEVSALWGEMDVINIAVAPEHRRRGVGQS
                     LMQWVIDLCQQLGIPLLWLRVRASNRVARRFYRRQGFRQRGRFEGYYQDPDEPAIIMA
                     MDVGAPNF"
     gene            complement(725..1777)
                     /locus_tag="AC16G0_14305"
     CDS             complement(725..1777)
                     /locus_tag="AC16G0_14305"
                     /inference="COORDINATES: protein motif:HMM:NF014653.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HIR5100822.1"
                     /translation="MAAEQVTVSAEAARLFCQEVLERAGLPKEDAFLVADALVDADLR
                     GVSTHGLVRFPIYVARLRKRLVNPTPRMRPIRERGATLVLDGDDGFGQVVGAEGMRRA
                     VLLAREHGVGMCAIRNGNHLGALAYIAAMAVPHHMIGITLTITNPVIAPWGGIVPKLG
                     NNPLAIAIPAGDRPPIILDMACSQVARGNLILASKLHQAIPPTWALDEKGRPTQDADA
                     GLRGSLMPVGGHKGSGLALTVGILGGLLAGSAFGAGLGDIFDMTRPQRFGHLILAIDV
                     SACRPVPEFTQGVEEMVQDLKATPKVEGIEEIYMPGELEARRREERLRHGFPVSPVVL
                     DEVKAVGAEFGVQWPA"
     gene            complement(1821..3566)
                     /locus_tag="AC16G0_14310"
     CDS             complement(1821..3566)
                     /locus_tag="AC16G0_14310"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5100823.1"
                     /translation="MALEIAPAKLRGVLEIVRLANAETAPGSMIALITEQACALLEAD
                     RGTLYLLDKETGELHSSIAMGIPGKTIRLKLHQGIAGLVAATGQPTLVEDAYTDTRFY
                     RGVDDATGYRTRQILCVPIKNRRGEVQGVLELLNKRTGPFTPGDEEILSLLASQVGVA
                     LANAQLYDGLRTNLDRLSRLMRIGTAISSELDLDDLLRTISETTSHLLQAERSTVFLV
                     DRARNELWSRVAQGLDREEIRIPLNTGIAGMVASTGTPVRISDAPTDPRFNPEVDKRT
                     GYQTLTILCAPMRDPHGQVIGVFQVLNKRGGQFSALDEQLLASLSSQAAVAVSNAQLY
                     DELQRSYTHLRTAEKMKSDFLGSLSHELRTPLTPILGYAEILLSGGMGTLPAGVIPGI
                     QAIDQSGKRMLHTVENLLAFIRLDQGEVTLNREPVHLDSLFAGLLEAFRGRAEEQKLH
                     LSREVAEDLPPVLADPQELRLALMHLVDNATKFTPAGGSVSLKAKTVAGDGGLPEVEI
                     AIQDTGIGIPPDLQPRIFDRFFQVDISPARQFGGVGIGLAVVKQIIEAHGSRVLVDSE
                     PGKGSTFTLRLPVGV"
     gene            complement(3723..4622)
                     /locus_tag="AC16G0_14315"
     CDS             complement(3723..4622)
                     /locus_tag="AC16G0_14315"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR5100824.1"
                     /translation="MDAQIGLVRALVPTTLALHVEIPAEHPSVTTLGEDRMGSAVLVD
                     SQGILLTVNYVVIGGRRITATLPDGRQYPAEIVAQDFETGLAALSVPLRDQPAATPGD
                     SRSLVTGLEVFILASAGPSERRASSGVITNLGPFDAYWEYLLESAIQTSAVNPGFGGG
                     PLFDIRGRVVGITSLNLGQVGRLSLAIPIHLYTEHRDDLLRFGRVRDRIQRAWVGFYP
                     EPGPSGIVVAGLVPEAPATRSGLREGDVILAVNACEVRTREELYREMWKVPAGDPLRF
                     RIQRGGQRTTVEVVSADRAEFYR"
     gene            complement(4813..5736)
                     /gene="trxB"
                     /locus_tag="AC16G0_14320"
     CDS             complement(4813..5736)
                     /gene="trxB"
                     /locus_tag="AC16G0_14320"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR5100825.1"
                     /translation="MRQAIILGSGPAGLTAAVYAARAHLKPLVVEGHEAGGQLTLTTL
                     VENFPGFPDAVMGPDLIKAMRSQAERFGADFRQGDATAVDLLRRPFRLTIDGQQEECQ
                     ALIIATGASAKLLGLDSERKLMGHGVSTCATCDGFFFQDQEVIVVGGGDSALEEAIFL
                     TKFATRVILVHRRDALRASKIMQDKAFKNPKIEFVWDSTLDHILDIQQQKVTAARIRN
                     LKTGAVTERPVDGIFIAIGHTPNTQLFRGVLDMDEVGYIRTHDGTKTSVPGVFAAGDV
                     QDHVYRQAITAAGSGCMAAIDAERFLEHQAH"
     gene            complement(5830..>6219)
                     /locus_tag="AC16G0_14325"
     CDS             complement(5830..>6219)
                     /locus_tag="AC16G0_14325"
                     /inference="COORDINATES: protein motif:HMM:NF015823.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HIR5100826.1"
                     /translation="YRVQYYAGDRWESPPADMVYQVLLDQFSGSGLFRRVIPWRRGEA
                     EYRLEARLRRFEELDEGEGWFGLVELEYEVADTHGRSLLRAVSSRRVRAEARSVEGVV
                     EALSRGLQASLGEATARTAEAIRKVQP"
BASE COUNT          992 a         2116 c         2074 g         1037 t
ORIGIN      
        1 gtctgagcgg gacgagcgag cgccttcagg tcgtccaggg acaccatgag gaatcctcct
       61 gtgatcacac atcacaccgc gtgctgttgc acatcatatc aacaaagaag cgaggccgtc
      121 cagccacccg gcggcggccg gagatttgtc cggagttccc taccggccgc catggcctgc
      181 cgtctgccga cgccggcaaa ggggaggtca gaagttggga gcgcccacat ccattgccat
      241 gatgatggcg ggttcgtcag ggtcctggta gtagccctcg aagcggccgc gctgccggaa
      301 gccctggcgc cggtagaacc gacgggcgac ccggttcgag gccctgaccc gcagccacag
      361 cagggggatg cccagttgct ggcagagatc gatgacccac tgcatcaggg attgtcccac
      421 cccccgccgg cgatgttctg gcgctaccgc aatgttgatc acgtccatct cgccccagag
      481 ggcgctcacc tcgacccccg cgtagccaag cagttcgccg gtgtcgggat cctcggcaac
      541 ccacatctca ccccgggtgg gctccgcctt cgagaaggcg gcgagcaact ccttgaaggt
      601 ctgaaagggc caggcctcct tgccaaagca gacggcctcg atggcgacaa cccggcccag
      661 gtcgcgcgcg gtcatggggc ggatacggag ggcggagggc ggggcgggcc tcatgcgggg
      721 ctgctcacgc cggccactgg acgccgaact ctgcgcccac ggccttcacc tcgtcgagga
      781 ccaccggact caccgggaag ccgtggcgca gccgctcctc ccggcgccgc gcctccagct
      841 cgcccggcat gtagatctcc tcaatgccct cgaccttcgg cgtggccttg aggtcctgga
      901 ccatctcctc gacgccctgg gtgaactccg gcaccggacg gcaggcgctg acgtcgatgg
      961 ccaggatgag gtggccgaag cgttggggcc tcgtcatgtc gaagatatcc cccagccccg
     1021 ccccgaaggc ggagcctgcc agcagaccgc ccaggatgcc aaccgtgagg gccaagccgg
     1081 atcccttgtg tccacccaca ggcatgagcg agcccctgag gcctgcgtcg gcatcctggg
     1141 tgggccgccc cttttcgtcc agcgcccagg tagggggaat ggcctggtgg agcttcgacg
     1201 ccaggatgag gtttccccgg gcgacctgac tgcaggccat gtccaggatg atggggggac
     1261 gatcgcctgc cgggatcgcg atcgccaggg ggttgttccc gagcttgggg acgatgccgc
     1321 cccagggcgc gatcaccgga ttcgtgatgg tcaaggtgat cccgatcatg tggtggggca
     1381 ccgccatggc ggcaatgtag gccagggcgc ccaggtggtt gccgttgcgg atggcgcaca
     1441 tgcccacccc gtgctccctc gccagaagga cggcccgtcg catcccctcg gccccgacca
     1501 cctgtccgaa gccgtcatcc ccgtccagga ccagcgtggc gccccgctcc cggattgggc
     1561 gcatccgggg agtcgggttc accagccttt tccgcaggcg ggcgacgtag atcgggaagc
     1621 ggaccaggcc gtgggtggag acgccccgca ggtctgcgtc caccagggcg tcggccacga
     1681 ggaaggcgtc ctccttgggg aggccggctc gctccaagac ttcctgacag aagaggcgtg
     1741 cggcctcggc ggagacggtc acctgctcgg cagccatggg ttgctccgat tccgtgtggg
     1801 caaggtcgcc ccgtcgcccg ttagaccccc acgggcagtc tgagggtgaa tgtcgaaccc
     1861 ttgcccggct cgctgtccac cagcacgcgg ctgccatggg cctcgatgat ctgcttgacc
     1921 accgccaggc cgatccccac acccccgaac tgccgcgcgg gggagatgtc gacctggaag
     1981 aagcggtcaa agatccgggg ctgcaggtcc ggtgggatgc cgatccccgt gtcttggatg
     2041 gcgatctcta cctcgggcag cccgccgtcg ccggccaccg tcttggcctt caggctgacg
     2101 ctgccgcccg ccggtgtgaa cttggtggcg ttgtccacaa ggtgcatgag ggccagcctg
     2161 agctcctgcg ggtcggccag gaccggtggc agatcctcgg ccacctcccg gctgagatgg
     2221 agcttctgtt cctccgccct tccccggaag gcctccagga ggccggcaaa gagcgagtcg
     2281 aggtggacag gctcccggtt cagcgtgacc tctccttggt ccagacggat gaatgccagg
     2341 aggttctcga ccgtgtggag catccgtttt ccgctctggt cgatggcctg aatgccggga
     2401 atcacaccgg cgggcagcgt tcccatgccc ccggagagca agatctcggc ataacccagg
     2461 atgggcgtga gaggggtccg cagctcgtgg gagaggctgc cgagaaagtc cgacttcatc
     2521 ttctccgcgg tccggaggtg cgtgtaggag cgctgcagct catcatacag ctgggcgttc
     2581 gacaccgcca cggcagcctg ggaggacagg ctggcgagca gttgttcgtc cagcgcgctg
     2641 aactgcccgc ctcgcttgtt caagacctgg aagacgccga tcacctggcc gtggggatcc
     2701 cgcatggggg cgcacaggat cgtgagggtc tggtaccccg tccgcttgtc cacctcggga
     2761 ttgaagcggg ggtccgtggg ggcgtcgctg atccgcaccg gcgtccccgt ggaggccacc
     2821 atccccgcaa tgccggtgtt cagggggatg cggatctcct cccggtccag cccctgcgcc
     2881 acgcgcgacc acaattcgtt cctggcccgg tccaccagga agaccgtgct ccgctctgcc
     2941 tgcaggagat gggaggtcgt ctccgagatg gtccggagca ggtcatcaag gtccagctcg
     3001 gagctgatgg cggtcccgat ccgcatgagg cgggagagcc ggtccaggtt ggtccggagc
     3061 ccgtcgtaga gctgggcgtt cgccagggcg acgccgacct gggaagccag caggctcaag
     3121 atctcctcgt cccctggcgt gaaggggccc gtgcgcttgt tgaggagttc gaggacgccc
     3181 tggacctccc cccgccggtt cttgatgggc acgcagagga tctgacgggt ccggtagccg
     3241 gtcgcatcgt ccacgcctcg gtagaagcgg gtgtccgtgt aggcgtcttc taccagggtg
     3301 ggttgtcccg tggccgccac caggcccgcg ataccctggt gcagcttgag tcggatggtc
     3361 tttcccggaa tccccatggc gatggacgag tggagttcgc ccgtctcctt gtccaggagg
     3421 tacagcgttc cccgatcggc ctccagcagg gcgcaggcct gctcggtgat gagcgcgatc
     3481 atggagccgg gcgccgtctc ggcgttggcg agcctgacga tttccaggac gccccgcagc
     3541 ttggccggtg cgatctcgag tgccatgatg cctcccgctc ctgtgtgggg gtgtcgaagc
     3601 ccggggcacg ccccccccat cggactgcgt cacatcctct tttcggccgc ccgggtgcgc
     3661 cgcttcggga cgcgcggacg ggccgtctcg gcttccccct tccgccgaga agggggggcg
     3721 gcctagcggt aaaattccgc gcgatcagcg ctcaccacct cgacggtggt gcgttggccc
     3781 ccccgctgta tgcgaaagcg cagcgggtcc cccgccggga ccttccacat ctctcggtac
     3841 agctcctcgc gggtgcggac ctcgcatgcg ttgactgcca ggatcacatc tccctcgcgg
     3901 aggccggatc gggtggcagg ggcctctggc acgaggccgg ccaccacgat ccccgacgga
     3961 ccgggctcgg ggtagaagcc gacccaggcc cgttggatgc gatcccgcac ccggccgaag
     4021 cggaggagat cgtcccggtg ctcggtgtag aggtggatcg gaatcgccag gctcaggcgg
     4081 cccacctgac ccagattcag cgaggtgatg cccaccaccc gtccccggat gtcgaagagc
     4141 gggccgccgc cgaatccggg gttgaccgcg gaagtctgga tcgccgactc cagcaggtat
     4201 tcccagtacg cgtcgaaggg gcccaggttc gtgataaccc cgctactcgc ccgtcgctcg
     4261 ctcggtccag cgctggcaag aatgaatacc tccagtccgg tcaccaggct gcgcgagtcc
     4321 cccggcgtag ccgccggctg gtcccggagg gggacggaca gcgcggccag accggtctcg
     4381 aaatcctgag ccacgatttc cgctggatat tgacgtccat cgggaagtgt cgcggtgatc
     4441 cggcggccgc ctatcaccac gtagttcaca gtcaggagaa tcccctggct gtccaccaga
     4501 accgcggagc ccatccgatc ctcgccgagg gtggtcacgg atggatgctc tgcgggaatc
     4561 tcgacgtgga gggccagcgt cgtcgggacc agggcgcgga ccagaccaat ctgagcgtcc
     4621 atgtttgcct acccggcaat cgggcgatct ggcaatccgg cgatcaggca cccaaggccc
     4681 gtgatccttt tgctcgggcg acagtgtgcc aaaggctgac agtctgctga aaaaggcgtc
     4741 gtcgaccagg ctgctcagga agatccagat gcaagccggc gcacccgccg atcggccttt
     4801 ctcggcggcc ggctaatgcg cctggtgctc cagaaaccgc tcggcgtcga tggcggccat
     4861 gcaaccggag ccggccgcgg tgatcgcctg gcggtacacg tggtcctgca cgtccccggc
     4921 ggcgaacacg ccgggaacgc tggtcttggt gccgtcgtgg gtccggatgt agcccacctc
     4981 gtccatgtcc agcaccccgc ggaagagctg ggtgttgggc gtgtgcccga tggcgatgaa
     5041 gatcccgtcc accggccgtt cggtgacggc accggtcttg aggttccgga tccgggccgc
     5101 cgtcaccttc tgctgctgta tatccaggat gtgatccagc gtcgagtccc agacgaactc
     5161 gatcttcggg ttcttgaagg ccttgtcctg catgatcttg gaggcccgga gggcgtcgcg
     5221 ccggtgcacc aggatgaccc gggtggcgaa cttcgtgagg aagatggcct cttccagggc
     5281 cgagtctcct ccgccgacga cgatgacctc ctggtcctgg aagaaaaagc cgtcgcaggt
     5341 cgcgcaggtc gagaccccgt ggcccatgag cttccgctcc gagtccagcc ccaggagctt
     5401 ggccgaagcg cccgtggcga tgatgagggc ctggcactcc tcctgctggc cgtcgatggt
     5461 gaggcgaaat ggacgccgca gaagatccac ggccgtcgcg tccccctgac ggaagtcggc
     5521 gccgaagcgt tccgcctggc ttcgcatggc cttgatcagg tcggggccca tgaccgcgtc
     5581 ggggaacccc gggaagtttt ccaccagggt ggtcaaggtg agctgccctc cggcttcgtg
     5641 gccctccacc accaggggct tgaggtgggc cctggccgca tacaccgcgg ccgtgagacc
     5701 cgccggtccg gagcccagaa tgatagcctg gcgcatgatc gaagacctcc tcgcgcgttg
     5761 ccggttgata ccgcactgct tgacccggat gattcacgaa cgggagcccg aatgatccgg
     5821 atcgactgac tagggttgga ccttccgaat cgcctccgcg gtcctggcgg tggcctcgcc
     5881 caggctggcc tggagaccgc gactcagcgc ctcgacgact ccctcgacgc tacgcgcctc
     5941 cgcccggacc cgccggctac tgaccgcccg cagcaggctc cgtccatggg tgtccgccac
     6001 ctcgtattcc agctccacca gaccaaacca gccttccccc tcgtccagtt cctcgaaccg
     6061 gcgcagccgg gcctcaagtc tatactccgc ctcgccgcgg cgccagggga tcacgcgtcg
     6121 gaacaagccg ctcccgctga actgatccag caacacctga tacaccatgt ccgcgggtgg
     6181 agactcccag cggtcgccgg catagtactg cacccggta
//
LOCUS       DBNGZC010000833         1604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1833818, whole genome shotgun sequence.
ACCESSION   DBNGZC010000833 DBNGZC010000000
VERSION     DBNGZC010000833.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1833818"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="AC16G0_14330"
     CDS             complement(<1..1108)
                     /locus_tag="AC16G0_14330"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR5100827.1"
                     /translation="MEEITVTRWLKQEGDSIKESEPLLEANTDKVDIEIPAPASGTVL
                     KILAAEGLPAKVGSLLAIIGNPGESLEAASSTASGMIPAQPAPALQELPVSAPLSTSP
                     APNPPKELGFVSPVVAKMAAEHSVDLSRVRGTGLNGRITKNDVLKFIETPQPENRPLP
                     APSSQAAPAAGDQLIKHTVIRRQIAEHMLASKHASPHVLTVMEADMGRVVAHRNANKE
                     AFARDGANLTFTVYFIAAIVAGLKAQPLANASWTEDGVLLHHAINIGMATSLGAEGLI
                     VPVIKGADGLSLLGLARAVNDLSSRARARKLQPDEVHGGTFTLTNHGTAGSLFAMPII
                     NQPQSGILGTGVMQKRAVVVTDSSGGDSIAIRPMV"
     gene            complement(1177..>1604)
                     /locus_tag="AC16G0_14335"
     CDS             complement(1177..>1604)
                     /locus_tag="AC16G0_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002695116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HIR5100828.1"
                     /translation="TIRAGGDLTLVTYGACCRIALDAADKLSQVGIQAEVIDVQSLLP
                     FDIHGRILESLKKTNRVLFVDEDVPGGTSAYMLQQVIEQQGGYSWLDSPPRTLPGAPH
                     RPAYGSDGDYWSKPNVETIFDAAYEMMHEVDPAAYPSLG"
BASE COUNT          291 a          461 c          554 g          298 t
ORIGIN      
        1 agaccatggg acggatggcg atggaatccc cgccgctgga atcagtgacg accacggcgc
       61 gtttctgcat aaccccggtg ccgaggatgc cggattgcgg ctggttgatg atgggcatgg
      121 cgaacagcga cccggccgtg ccgtgattgg tgagggtgaa tgtgccgccg tggacttcgt
      181 cgggttggag cttcctggcg cgggcccggc tggagaggtc gttgaccgcg cgggcgaggc
      241 ccagcagcga caggccatcg gcacccttta tcaccggcac aatgagcccc tccgcaccaa
      301 gcgaggtggc catgccaatg ttgatggcat ggtgaaggag gacaccatcc tcggtccagg
      361 aggcgttcgc caggggctgg gccttgagac cggcgacaat ggcagcaata aagtaaacgg
      421 tgaaggtcag attggccccg tcgcgagcga aggcctcctt attggcattg cggtgcgcga
      481 ccacgcggcc catgtcggct tccatgacgg tcaggacatg aggagaggcg tgcttggagg
      541 cgagcatgtg ctcagcgatc tgcctgcgga tgaccgtatg cttgatgagc tggtcgcctg
      601 cagcgggggc ggcttgcgac gaaggagcag gaagcggtct attctccggc tggggagtct
      661 cgataaactt cagcacgtcg ttcttggtaa tgcgcccatt caggccggtg cctcgcaccc
      721 gtgacaggtc cacactatgc tcggcggcca tcttggcgac cacaggggat acgaagccca
      781 actctttggg cgggttcgga gccggagatg ttgaaagcgg ggcgctgacc ggaagttcct
      841 ggagggctgg cgcgggctgg gccgggatca tgccagaggc agtggaactg gcagcctcca
      901 gcgattcgcc cgggttgccg ataatcgcga gaagcgaccc aaccttggcc gggaggccct
      961 ccgccgcaag gatcttgagc accgtaccgg aggcgggagc ggggatctcg atatcgacct
     1021 tgtcggtgtt ggcctccagc aggggctcgg actccttgat ggagtcaccc tcctgcttta
     1081 gccaccgggt aaccgtgatc tcctccacgc cttcacccgg caatgggact aatactctgg
     1141 ttgccatgct ttctccggtt ggtggttggt cgtcgttcag ccgagcgacg ggtacgccgc
     1201 cggatcgact tcgtgcatca tctcgtaggc cgcatcgaag atggtctcga cgttgggctt
     1261 ggaccaataa tccccatccg agccataggc aggacgatgg ggcgctccgg gcagggtgcg
     1321 gggcggggaa tccagccagg agtagccgcc ctgctgctcg atgacctgct gaagcatgta
     1381 ggcgctggtg ccacccggca catcctcatc cacgaagagg acgcggtttg tcttcttcaa
     1441 tgattcgagg atgcggccgt ggatatcgaa ggggagcagg gattgcacgt cgatcacctc
     1501 agcctggata ccgacctgcg agagcttgtc cgcggcgtcc agggcaatgc ggcagcaggc
     1561 cccataggtg acgagggtca gatcgccacc cgcgcggatt gtct
//
LOCUS       DBNGZC010000834         4509 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_462090, whole genome shotgun sequence.
ACCESSION   DBNGZC010000834 DBNGZC010000000
VERSION     DBNGZC010000834.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_462090"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..1413)
                     /locus_tag="AC16G0_14340"
     CDS             complement(175..1413)
                     /locus_tag="AC16G0_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HIR5100829.1"
                     /translation="MSVRWSRIRRRAFLFLMVMGPGIITANIDNDAGGITTYSIAGTQ
                     YGYGLLWSLIPTTLSLIVIQEMCSRMGVVSGKGLAELIRERFGVRATLAVMACLVLAN
                     LANTAAEFAGVAASLEIFGIGKLVGVPLVALLVGWLVVKGSYRFVERVFLLASVVYLV
                     YIPSAFLANPPWGDVFRQTFHPVFRLETGYVTTLVTVIGTTIAPWMQFYQQSSVADKG
                     IRIKEYRYARWDVILGGFMTDIVAFFIIVACAATLFTHGVPIQSAKDAALALEPFAGR
                     YASLLFGVGLLNASVFASAVLPLSTAYAVCEGLGWETGISRTLREAPAFFGIYIGMIV
                     LGAGLILLPRAPLLQVMFLSQTLNGLLLAPVLIFMLILINDRDVMGNHVNSVLFNLIA
                     WGTAGIMIVLSGLLLITSVM"
     gene            complement(1519..2793)
                     /locus_tag="AC16G0_14345"
     CDS             complement(1519..2793)
                     /locus_tag="AC16G0_14345"
                     /inference="COORDINATES: protein motif:HMM:NF015413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR5100830.1"
                     /translation="MLATLSFTQIRHRPVQDAEGTPVGHLEDLVVLALGAKPLVTKFS
                     LRLPDQEERILPWDLVSELPTDPHAPIRLRSRSADLTAVPLRPDEMRLAKNVLDKKVV
                     DTARKRVVRVNDIELEGREGRLHVTGVEGGLRGLLRHLRSEALAERLAALFGGNLPRD
                     LVPWEALDPLENELTRAKRQAVYTKLAKLHPADIADIMEELNPSERAAILAALDESTA
                     AEAITEAEPEVQASVVQMMEPEKAADILEEMEPDEAADILHDLPEAKAEELLETMEPE
                     EAQEVEELLEHEEDTAGGLMTTEHVAFAPALTAAEAIQRIRDEAREAETIYYLYVTGP
                     QERLLGVLSLRELILADPGQRLERIMHTEVVSVRPETGLREVAELQTKYNLLAVPVVS
                     GEGEMLGIITVDDVLNLILPMIWKKRAVKKFI"
     gene            complement(2803..3438)
                     /locus_tag="AC16G0_14350"
     CDS             complement(2803..3438)
                     /locus_tag="AC16G0_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100831.1"
                     /translation="MASRGDVSFYRDLLHKEVVDCHGHRIGAVLDLAAGPCQSLPGNP
                     AIQRLVIRPHRITGSEPVAAPKTPLILPWELVEALEPRHIRLSRACAELSHSSLEAGQ
                     ILLRRQIMDQQVVDCRGLKLQRVNDITMGYSDGALCLWGMDTGFSGFLRRLGDRWGLF
                     DWLPPFRRRLWRHVIRWDFVDRLEPTRGHIRLRLSRDEVRSAVRHAPDSVA"
     gene            3695..4072
                     /locus_tag="AC16G0_14355"
     CDS             3695..4072
                     /locus_tag="AC16G0_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012909527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5100832.1"
                     /translation="MEVVNRDRSLPVITKDGSEIRSILDRTNSGATNQSLAEATVPVG
                     AETEAHYHSRSEEIYYVLGGRGLMTLGDERREVRQGDGILIPPGTCHKIRNIADEPLV
                     ILCCCAPPYSHQDTILVADRDGG"
     gene            complement(4118..>4509)
                     /locus_tag="AC16G0_14360"
     CDS             complement(4118..>4509)
                     /locus_tag="AC16G0_14360"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR5100833.1"
                     /translation="NAHQLIQTDSGPFAIAGIDDLGTGRPNLHAALRGLQPSIPTLLL
                     SHRPEIFPQAAGRNIPLTLAGHWHGGQIKLSVLGMDLSVAQFLSPYPEGLFRIDSSYL
                     YVNRGIGTTGPPIRLNASPEVTLFQLT"
BASE COUNT          780 a         1439 c         1530 g          760 t
ORIGIN      
        1 tacaagcctt cgggagccag ttctcctggt ccattcaccc gatggtcacc cgatggagcg
       61 cccgcccgcc tgcccgccgg ccggtttcgc gagtttttgc gcctcagaat atcaggcttg
      121 gcccaagggt ccaacgggtt tcttcccggc gcccccggtc cgctggcctg ggcctcacat
      181 cacgctggtg atcagaagca gcccgctgag gacgatcatg atgcccgcgg ttccccaggc
      241 aatcaagttg aagaggacgg agtttacgtg gttccccatc acatcccgat cgttgatcag
      301 gatcagcatg aagatgagga ccggggcgag gagcaggcca ttcagcgtct gcgacaggaa
      361 catgacctgg aggagcggcg cgcggggcaa gaggatcagc cccgccccca gcacgatcat
      421 gcctatgtag atcccgaaga acgcgggcgc ctcccgcagc gtgcgcgaga tccccgtttc
      481 ccacccgaga ccctcgcaga ccgcgtaagc cgtggagagt ggcaagacgg ccgaggcgaa
      541 gaccgaggcg ttgagcaggc ccacgccgaa gagcaggctg gcgtatcggc cggcgaacgg
      601 ctccagcgct agcgcggcat ccttggcgct ctggatgggc acgccatggg tgaagagggt
      661 ggcggcgcaa gcgacgatga tgaagaaggc cacgatgtcg gtcatgaacc cgcccaggat
      721 gacgtcccac cgggcgtagc ggtactcctt gatccggatg cctttgtcgg ccaccgagga
      781 ctgctggtag aactgcatcc agggggcgat ggtggtcccg atgaccgtga cgagcgtcgt
      841 cacatatcct gtttccaggc ggaagaccgg gtggaacgtc tgccggaaga cgtcacccca
      901 gggtggattg gcgaggaacg ccgaggggat gtacacgagg tagacgacgc tggccagcaa
      961 gaagacccgc tcgacgaacc ggtagcttcc cttcaccacc agccagccca cgagcagggc
     1021 aaccagcgga acgcccacca gcttgccgat accgaaaatc tccaggctgg ccgccacacc
     1081 cgcgaactcc gccgccgtgt tggccaggtt cgcgaggacc aggcaggcca tcaccgcgag
     1141 ggtggcgcgg acgccgaacc gctcgcggat cagttcggcc aggcccttgc cgctgaccac
     1201 ccccatgcgg ctgcacatct cctggatgac gatgaggctc agggtggtgg ggatgagcga
     1261 ccagagaaga ccgtagccgt actgcgtccc ggcgatggaa taggtggtga tcccgcccgc
     1321 atcgttgtcg atgttggccg tgatgatccc cggccccatg accatgagga agaggaaggc
     1381 acggcggcgg atgcggctcc agcgtacgga catagctcgg ccaggactcc gacctgccgg
     1441 cagcacgctg ccgggagggc cgcgccccga cgggtcgagg cgggggcagt cgcacaccag
     1501 gggatcgtcc ggcgaccgct agatgaattt ttttactgcc cgcttcttcc atatcatcgg
     1561 gaggatgagg ttcaggacat cgtccaccgt gatgatgccg agcatctcac cctcgccgct
     1621 caccaccggc acggccagca ggttgtactt cgtctggagc tccgccacct cgcgcagccc
     1681 ggtctcgggc cggacgctga cgacctcggt atgcatgatg cgctccaggc gctggcccgg
     1741 gtcggccagg atcagttctc gcaggctcag gacccccagc agccgctcct gggggccggt
     1801 cacgtacagg tagtaaatgg tctcggcctc cctggcctcg tcccggatcc gctggatggc
     1861 ctcggccgcc gtgagcgcgg gggcaaaggc cacgtgctcg gtggtcatga ggccgccggc
     1921 cgtgtcctct tcgtgctcca ggagctcttc cacttcctgg gcttcctccg gctccatggt
     1981 ctccagcagt tcctcggcct tggcctcggg caggtcgtgg aggatgtcgg cggcctcgtc
     2041 cggctccatc tcctccagga tatccgccgc cttctcgggc tccatcatct ggacgaccga
     2101 ggcctggacc tctggctcgg cctccgtgat ggcctcggcg gcggtggact cgtccagcgc
     2161 cgccaggatg gcggcccgct cgctggggtt caactcctcc atgatgtcgg cgatgtcggc
     2221 tgggtggagc ttggccagct tggtgtagac cgcctgccgc ttcgcgcggg tgagttcgtt
     2281 ctccagcggg tccagggctt cccacggcac caggtcgcgg ggcaggttcc ccccaaagag
     2341 cgcggccagg cgttccgcca gggcctccga ccgcaggtgc cgcaggaggc cgcgcagccc
     2401 gccttcgacc ccggtcacgt gaagccggcc ctcgcgcccc tccagttcga tgtcgttgac
     2461 ccggacaacc cgcttgcgtg cggtgtccac caccttctta tccagcacgt tcttggccag
     2521 gcgcatctcg tccggacgaa gggggactgc ggtcaggtcg gccgaccgac tgcgcaggcg
     2581 gatgggggca tggggatccg tggggagttc gctcaccaga tcccagggca ggatcctttc
     2641 ctcctggtcc ggcaagcgca ggctgaactt tgtcaccagg ggcttggcgc cgagggcgag
     2701 gaccacgagg tcctcgaggt gccccacggg cgtgccttcg gcatcctgca ccgggcgatg
     2761 gcggatttgc gtgaaagaga gtgtggccag catacgggcg acctaggcga cggaatcggg
     2821 ggcgtggcgg accgcagagc ggacctcgtc acgcgagagg cggaggcgga tatggccccg
     2881 ggtcggctcc agccgatcca caaaatccca gcggatcacg tgccgccaca ggcgtcggcg
     2941 gaaggggggg agccagtcga agagacccca ccgatcgccc agacgccgga ggaatccgct
     3001 gaaacctgtg tccatacccc aaaggcaaag ggcgccgtcg gagtacccca tggtgatgtc
     3061 attcactcgc tgaagcttca ggccgcgaca atccacgacc tgctggtcca tgatctgtct
     3121 gcggaggagg atctggccgg cctccaagga cgagtggctc aactcggcgc atgcccggga
     3181 gagtcgaatg tgtcgcggtt ccagggcctc gaccagctcc caggggagga tcagcggcgt
     3241 cttgggtgcg gccacgggtt cgctgccggt gattcggtgc ggccggataa cgaggcgttg
     3301 gatggcgggg ttgccaggaa ggctttggca tggcccggcg gcaaggtcca agaccgcgcc
     3361 gatccggtga ccatggcagt ccacgacttc tttgtggagg aggtcccggt agaagctcac
     3421 gtcgccccgg ctcgccatga gtcgcggccc ccttccggtt cggccgaacg ttccctccgc
     3481 cttgtctccg ccttcgcgcc gccgccggtt ctttgtacag cgcggtcggg aagctgtcaa
     3541 ccctcatttg cggcgcctcg tatggttttt cagcctttcc aaaccgcttg tccggccggg
     3601 agaagactgc tatcctgccc ggcgaatctc gacatcccat ccgcccgcag cggcttccga
     3661 cgttcctcag gggctgagag aggagtgaac ggccgtggaa gtggtgaacc gcgatcggag
     3721 cctccccgtc atcaccaaag atgggtccga gatccgctcg atcctggacc ggacgaattc
     3781 gggggcgacg aatcaaagcc tggccgaggc gactgtgccc gtcggtgcgg aaacggaagc
     3841 gcactaccac tcacgcagcg aggaaattta ttacgtgctt ggcgggcgag ggctcatgac
     3901 cctcggtgac gagcggcgag aggtccggca gggagacggg attcttatcc cgccggggac
     3961 ttgccacaag attcggaaca tcgccgatga acccctggtc atcctgtgct gttgtgcgcc
     4021 gccatattcc caccaggaca cgatcctggt ggccgacagg gacggaggat gaaagcgttc
     4081 gggtcgtgta tgccggacct tggtggaggg gacagcctca ggtcagttgg aagagcgtca
     4141 cctcggggga ggcgttcagg cgaatcgggg ggcccgtggt accaatccca cggttcacgt
     4201 acagatagga tgagtcgatg cggaagaggc cctctggata gggactgagg aattgggcca
     4261 cgctcaggtc catcccgagg acgctcagct tgatctgccc cccgtgccag tggcccgcca
     4321 gggtcagggg gatattgcgg ccggccgcct gcggaaagat ctcggggcga tgcgacagga
     4381 ggagggtcgg gattgatggc tgcaggcctc gcaaggccgc atgcagattg ggtcttccgg
     4441 tgccaagatc gtcgatcccc gcgatggcga aggggcccga atcggtctgg atgagctggt
     4501 gggcattgt
//
LOCUS       DBNGZC010000835         3399 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2616275, whole genome shotgun sequence.
ACCESSION   DBNGZC010000835 DBNGZC010000000
VERSION     DBNGZC010000835.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2616275"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..1131
                     /locus_tag="AC16G0_14365"
     CDS             49..1131
                     /locus_tag="AC16G0_14365"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR5100834.1"
                     /translation="MGKGRTFLDALRAQGLRQDLASQLVQVFRPHVNFRRLKPEDSLE
                     IHHDSTGRVAKVVYRQSPIDIVEATPNGDAWTASRVDVPVESRVVVVAGTVSGNLFES
                     VEALGERPQLVLDFAEIFAWDFDFAADSQPGDRFRMLVEKVFTGDLFVKYGRILAAEY
                     ENEDTVHTGLYFKGKEGSGYYTPAGESLRRAFLKSPLEFTRISSGYSRSRRHPILGGV
                     RPHLAVDYAAPLGTPVWAVAEGRVEFAGWSAGNGNTVILRHRANFKTMYNHLSRFGRS
                     IRTGATVSQRQAIGYVGSTGLSTGPHLDYRVIKDGRFVNPLKQTYLPGNPISHSAHNA
                     FSEARDSLLRQLHGTEASQVARAPRS"
     gene            2363..>3399
                     /locus_tag="AC16G0_14370"
     CDS             2363..>3399
                     /locus_tag="AC16G0_14370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5100835.1"
                     /translation="MHVTELAAALERKGHEVHVFTRLGWGQSHLDRIHGVWYHRCPFD
                     LNRNFVDEIQNMCRSFVHYLFATEDYMGRPFDVIHAHDWLASNAMVWAKHGRGRKGIL
                     TIHSTEYGRCGNNFWGGPSARIRDHERHGTYCADRVITVSRALKGEVEWMYNVPDWKV
                     AVIYNGVNVHAYDGWIPDAGMIRRGYSVGPLDPMVLFSGRMTSQKGPDLLVEAIPMIL
                     RYYPRAKFVFAGEGDLRGPCERRAGQLGVAHACRFLGWRSGAELQSLYKISDAVCVPS
                     RNEPFGITILEAWSAGRPVVASKNGGPDEFVWHEVTGLKIYPTAESVAWGIGTLFTNF
                     DWARWMGQNGRV"
BASE COUNT          620 a         1063 c         1069 g          647 t
ORIGIN      
        1 cccctcccgc ctcctccccc cgcccaacct gctcccctct ccagcaaaat gggaaaggga
       61 cgcaccttcc tggacgccct gcgggcccag ggcctgcggc aggacctcgc ctcacaactt
      121 gtccaggtct tccgtcccca cgtgaacttc cgccgcctca agcctgaaga ttcgctggaa
      181 atccatcacg actccacagg cagggtggcc aaggtggtat atcgtcagag cccgatcgac
      241 atcgtggagg ccactcccaa cggtgatgcc tggactgcct cccgcgttga cgtcccggtg
      301 gaatctcggg tcgtcgtggt agccgggacc gtgtctggaa acctgttcga gagtgtggag
      361 gccctcggag agcggccgca actcgtcctc gacttcgccg agatctttgc ctgggacttt
      421 gacttcgccg ccgattcgca gcccggggac cgcttccgga tgctggtgga gaaggtgttt
      481 accggggacc tgttcgtgaa atacggccgc atcctggccg ccgagtatga aaacgaagac
      541 acggttcaca cggggctcta cttcaagggg aaagagggta gcggatacta cacgccggcg
      601 ggcgaatcct tgcggcgagc tttcctgaag tcgccgctgg agttcacccg gatcagctcc
      661 gggtacagcc gcagccgtcg acatccgatc ctgggcggcg tgcggcccca cctggccgtg
      721 gactacgccg cgcccttggg cacccccgtc tgggccgtgg ccgagggccg ggtggaattc
      781 gcggggtgga gcgcggggaa cggcaatacc gtgatcctcc ggcaccgggc gaacttcaag
      841 acgatgtaca accacctctc ccgcttcggc aggagtatcc ggacgggcgc cacggtcagc
      901 cagcgccagg cgatcggtta cgtgggctcc accggcctct ccacggggcc gcacctggac
      961 tatcgcgtca tcaaagatgg caggttcgtc aacccgctga agcagaccta tctcccgggc
     1021 aatcccatct cccactccgc gcataacgcc ttctctgagg cgcgtgattc tctcctgcgc
     1081 caactccacg ggacagaggc gtcgcaggtg gcaagagccc cgagatcctg acccagcctt
     1141 ttccttcccc cgtaactttg caggagagaa cgggagggca agggtccctg ccggcagcgc
     1201 ccaaggctca ggagcccccg ctacgaagcc agcgtgatct gctccagccc atccagattg
     1261 gtgagggcaa ggctccgact gccgccggtg atgagccgac gggaccgaaa atcggccaag
     1321 acgtagttga cgtgcttccg cgagaaggta gtggcgcccc tctagggcgc acccggaata
     1381 tcgcgtcacc tcacgtccct ccgcacggcg ccggtttccc cgccctcgat tcccgacagg
     1441 gatggcagca ccctgaggct tagtaaggct gaagcgggtt gatcgcacca agacgcacga
     1501 tgaatggttc ttgacaagcg gatgttgctt gcggatagta cacccgttat gcgctgggag
     1561 ctgcatcgtg ttgttccacg tcatcgcagg tgtggtttgg gggcggcaaa gcgcgatccg
     1621 tgattgggcc atggatgccc tggtttcccg tgatggaggc gggaccaggg cgtttttgtt
     1681 cggcgcactg tgtccccgct tcaagaacgt cgcggtcgcc aagaggggag gggaagcctt
     1741 cgacgtgtgg cagtcgggga cttctttgtg accgctcgag gctcggatca ctcataccca
     1801 taccctcgac gttcatttct tgggccgaac gacgtgggtc ccgccggtcc tgcggtccgg
     1861 acggagaccc cggactatag aacctgacct gcatctatag tttttctgac acccggagat
     1921 gagcctcgtc tcgtggggac gaggcgaact cgattcggcc cttccttgtt cacctgattg
     1981 cccgctctgc caggacatcc aggacgaaga aatcaggagt tcgccaccca agggcggtga
     2041 agggattcga gtccgccgcg gatagcagct cctcaaatcg tcttcaatcc tgacaatcct
     2101 caaggtgtcg tggcggagat ttccaccgaa gcggctggag ggcccccgca cctctgggct
     2161 ctccacgcca agtccgcttt ctagagatct gggtgatcgt cgtcggacgg cgtcccgcga
     2221 tcacgacgcg gcgagtgccc tgacccgaaa ttcgcggcca cgtggcggcc actgagtagc
     2281 catcaatgga aggtcgatgg tcatgagaat cgcattggtc agttgggagt ctcttcactc
     2341 gattccagta gggggggtgg ctgtgcatgt cacggaactg gccgccgcct tggagcgcaa
     2401 ggggcacgag gtgcacgtct tcacccgcct aggctgggga cagtcccacc tggatcggat
     2461 ccacggggtc tggtaccacc gctgtccctt tgacttgaac cgcaacttcg tggatgagat
     2521 ccagaacatg tgccgctcct tcgtccacta cctcttcgcc accgaggatt atatgggaag
     2581 gcccttcgac gtgattcacg cccacgactg gctggcctca aacgccatgg tctgggcgaa
     2641 gcatgggcgg ggcaggaagg gtatcctgac cattcattcg acggagtacg gtcgctgcgg
     2701 taacaacttc tggggcggac cgtctgcccg catccgcgat catgagcggc acgggaccta
     2761 ctgcgccgat cgcgtaatca ccgtgtcccg ggccctgaag ggtgaagtgg agtggatgta
     2821 caacgtcccg gactggaagg tggcggtcat ctataatggc gtgaatgtcc atgcatacga
     2881 tggatggatc cccgacgctg gcatgatccg ccgaggatac agcgtcggcc ccctcgaccc
     2941 catggtgctc ttctcgggac gcatgacctc tcagaaggga ccggatcttc tggttgaggc
     3001 cattcccatg atcctccgct attatcctcg cgccaagttc gtcttcgccg gggaggggga
     3061 tctgcgcggg ccttgcgagc ggcgagccgg gcagcttggg gtggcccacg cctgccgatt
     3121 cctcggatgg cggagtgggg cggagctgca aagtctctac aagatcagcg atgcggtctg
     3181 cgtgcccagc cggaacgaac ccttcggaat caccatactg gaggcctgga gtgcgggaag
     3241 acccgtggtg gcctccaaga acggaggacc cgatgagttc gtctggcatg aggtcaccgg
     3301 gctgaagatc tatccgacgg cagaatccgt cgcctggggg atcggcactc tcttcacgaa
     3361 tttcgattgg gcccgctgga tgggtcaaaa cggcagggt
//
LOCUS       DBNGZC010000836         3829 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_170524, whole genome shotgun sequence.
ACCESSION   DBNGZC010000836 DBNGZC010000000
VERSION     DBNGZC010000836.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_170524"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /gene="mrdA"
                     /locus_tag="AC16G0_14375"
     CDS             <1..1270
                     /gene="mrdA"
                     /locus_tag="AC16G0_14375"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR5100836.1"
                     /translation="ENLGQSGVERRFDGFIRGVDGGEQIEVDARGRALRLVNRIEPRS
                     GSNIILTIDKRIQEAAEAAFAGKKGAVVAMNPTTGEILAMVSRPSFDPNLFAQRLTGE
                     EWQQIATDPSHPLQNRAYQAQYPPGSIFKLVVAIAGLESGALTPETKFNCPGQFYLGN
                     VKFDDWKKGGHGTLDLRDAIKNSCNVYFYQAGLRVGIDEMVRVAKAFGLGAPPGLGLG
                     DEVKGNLPNPQPRKRGQPGWTPGNTVISSIGQGLVVTSPMQLLGLVSAIANGGTIYRP
                     WVVKQVVSLSGEVLEEYEPQAVRQVPVKPETLAFIRQAMLGVVEGGTGARAKVPGITI
                     AGKTGTAQVVKKGEGKGHADLKDHGWFVSFAPVDNPQIAVVVLVENGGFGGLVAAPVA
                     KAVYEAALGPNQTIAGASLGGSKEDSDLED"
     gene            1344..2441
                     /gene="rodA"
                     /locus_tag="AC16G0_14380"
     CDS             1344..2441
                     /gene="rodA"
                     /locus_tag="AC16G0_14380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIR5100837.1"
                     /translation="MIDRRLISYFDWRFAGTILAMAILGILSVYSANALGTSAFRKAL
                     YLRQMLWVGMGLVALTLACMVSYRTLARFAYVIFGFSLVLLLLVLVIGKAGLGAHRWI
                     RIAGFTFQPSEFAKLGLVLFLARYFDDHREALHQPRTMLLPAIMTGACMLLVLKQPDL
                     GTALLLVFISLTMMLMLGLRWRYLVPPLVAGAVLAPIAWTFLKDYQRRRLLVFVNPDL
                     DPLGASYHIAQSKIAVGSGGVFGKGWLAATQSQLNFLPLNHTDFIFAVLAEQWGFLGC
                     LVVLLVYFYLITRGFQIAQECTDLFASLLAAGVSAMLAIQVLINIAMVLGMMPVVGIP
                     LPLLSYGGSSLLVTMLSLGLLLNLHMRRFMY"
     gene            complement(2934..3710)
                     /locus_tag="AC16G0_14385"
     CDS             complement(2934..3710)
                     /locus_tag="AC16G0_14385"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HIR5100838.1"
                     /translation="MDLRSLRAFIFDLDGCVYTGNTLVPGVQAFLKELREKERQLLFV
                     TNNSREAGEELQAKLHRLGVRAGREEILSAAEIVGPLVRERFGPSRVLAVGSDTLQRL
                     LREAGHTLLPLEAYRDAQVVVVGHDFDFEYPKLTALSRAVVAGAVFLAVNVDPRLPVE
                     LGEFYPGCGSLVEAVAVAAGVRPEVVGKPMPHIFRAALTRLGVRPQDAVMIGDSLVSD
                     IQGAQGVGLWTIWLAPQGSGPGEVRPDLTIHSFGDVSGRL"
BASE COUNT          661 a         1262 c         1182 g          724 t
ORIGIN      
        1 ggagaacctc ggccagtccg gcgtcgagcg tcggttcgac ggcttcattc ggggcgtgga
       61 tggcggcgag cagatcgagg tggatgcccg gggccgcgcc ctgcgtctgg tcaaccgcat
      121 cgagccgcgc tccggctcca acatcattct gaccatcgac aagcgcatcc aggaggcggc
      181 agaggcggcc ttcgccggca agaagggagc cgtggtcgcc atgaacccga ccacgggcga
      241 gatcctggcc atggtgagtc gcccctcctt tgacccgaac ctcttcgccc agcgcctgac
      301 cggcgaggag tggcagcaga tcgccaccga cccttcccac cccttgcaaa accgggccta
      361 tcaggcccaa taccctccgg gctcgatctt caagctggtc gttgccatcg ccggtctgga
      421 gagcggggcc ctgacccccg agacgaaatt caactgcccc ggccaattct acctgggaaa
      481 cgtgaagttc gatgactgga agaagggcgg ccacgggacg ctggacctca gggatgccat
      541 caagaactcc tgcaatgtct acttctacca ggccggtctg cgggtgggca tcgacgagat
      601 ggtccgggtc gccaaagcct tcggtctcgg cgcaccgccg gggcttggcc tgggcgacga
      661 ggtcaagggg aatctcccca acccacagcc gaggaagcgc ggccagcccg gctggacccc
      721 cggcaatacc gtgattagca gcatcggcca gggattggtg gtgacttccc ccatgcagct
      781 tctcgggctg gtctcggcca ttgcaaacgg cgggacgatc taccggccct gggtggtgaa
      841 gcaggttgtt tccctctcag gcgaggtcct cgaggaatac gagccgcagg ccgtccgcca
      901 ggtcccggtc aaacccgaga ccttggcgtt catccggcag gccatgctgg gggtggtgga
      961 gggggggacg ggcgctcgcg ccaaggtgcc cgggatcacc atcgcgggca agaccggcac
     1021 ggcccaggtg gtgaagaagg gtgaggggaa gggccatgcg gatttgaaag accacgggtg
     1081 gttcgtctcc tttgcccccg tggacaaccc ccagatcgcc gtggtggtct tggtggagaa
     1141 cggcggcttc ggcgggttgg tggccgcgcc ggtggccaag gcggtatacg aggccgccct
     1201 gggcccgaac cagaccatcg ccggggcctc tttggggggg tccaaagagg attcggatct
     1261 tgaagattga tggaagcggt cagccctcag cggtcaggat ttctccgctg ttgaaggctg
     1321 atggctcata gctgagagct tccatgattg accgacgctt gatctcgtac ttcgactggc
     1381 gcttcgccgg taccatcttg gccatggcga tcctggggat cctttcggtt tatagcgcca
     1441 acgccttggg cacgtctgcc ttccgcaagg ctctctacct ccgtcaaatg ctgtgggtcg
     1501 gcatggggct ggttgcgttg accctcgcct gcatggtgag ctaccggact ctggcgcgct
     1561 tcgcctatgt gatcttcgga ttcagcctgg ttcttctcct cctggtgctg gtcatcggca
     1621 aggcggggct gggcgcccat cgctggattc ggattgccgg gttcaccttt cagccgtcag
     1681 agttcgccaa gctcggcctg gtgctcttcc tggcgcgata cttcgacgat caccgggaag
     1741 ccctccacca gcctcggacc atgctcctgc ccgccatcat gacgggcgcg tgcatgctgc
     1801 tcgtcctgaa acagccggac ctggggaccg ccctgctcct ggtgttcatc tccctcacca
     1861 tgatgctgat gctcggcctc cgctggcgat acctcgtccc tcccctggtc gccggcgccg
     1921 tcctggcccc aatcgcctgg accttcctca aggactacca gcgacggcgt ctcctggtct
     1981 tcgtgaaccc cgacctcgat cccttgggcg ccagctacca catcgcccag tccaagatcg
     2041 cagtcggctc cggcggggtt ttcggcaagg gctggttggc cgccactcag agtcaactga
     2101 acttccttcc cctgaaccac acggatttca tctttgccgt cctggccgaa cagtggggat
     2161 tcctgggctg cctggtggtg ctgctggtct atttctacct catcacgcgg ggcttccaga
     2221 tcgcccagga gtgcaccgat ctcttcgcct ccctcctcgc cgccggagtc tcggccatgc
     2281 tggccatcca ggtcctcatc aacatcgcca tggtgctggg catgatgcct gtggtgggta
     2341 ttccccttcc ccttttgagc tatggcggct cctccctgct ggtcaccatg ctgagcctgg
     2401 ggctcctcct caacctccac atgcgccggt tcatgtactg acccgactcc gagaagaatc
     2461 gcaccaccga gcgcccaagg caccagcaca gacgctgaca ttcagacttt gggctctgat
     2521 atgtagccac ggcctacagc ggccttcggc ttttcatatc cagacccttg ctggacaatg
     2581 gctttccctg gtgtcttcgt gccttggtgg tgagggaata tcaggcaaga cgaacacgag
     2641 gctgagaaat ccggacgctg tagcctcacg tgaggagggg gggcccgccg cagatgggca
     2701 cctaacggat cggtacccgg cagatgagtc tatcttcagc ccgaacctat caccgccaag
     2761 attgccaaga ccgcgaagac ccgccagccc tcatcaatac agaagaccat caggatcgaa
     2821 ctctttcgga cgttcatccg agcggttccc atcagcagtt tgaaatcgag atgttccttt
     2881 gcggtctttg cgcgctttgc ggttgaattg cattattcag gttcagcagc ctgctatagc
     2941 cgcccagaca cgtcgccgaa ggaatggatc gtcaggtccg ggcgtacctc gccggggccg
     3001 gacccctggg gagcgagcca gatcgtccag aggcccacgc cctgcgctcc ctggatgtcc
     3061 gagacgaggc tgtccccgat catgaccgca tcctgcgggc gcacgccgag tcgcgtcagg
     3121 gccgcccgga agatgtgagg catcggcttc cctaccactt cggggcggac gccggcagcc
     3181 acggccaccg cttcgacgag gctgccgcag ccgggataga attcccccag ctcgaccggc
     3241 agccgcggat cgacattcac cgccaggaaa acagctcccg caacgacggc gcgggagagg
     3301 gcggtcagct ttggatactc gaagtcgaag tcgtgtccca ccaccacgac ctgagcgtcg
     3361 cggtaggcct ccaggggcag gagcgtgtgg ccggcctccc gcagcaggcg ctggagcgtg
     3421 tcggaaccga cggccaacac ccgcgacggg ccgaagcgct cccgcaccaa gggaccgacg
     3481 atctccgcag ccgagagaat ctcctctcgc ccggcgcgca ccccgagacg gtggagcttt
     3541 gcctgcagct cctcaccggc ctcccgcgaa ttgttggtga cgaacaggag ctgccgttct
     3601 ttctcccgca actctttcag aaacgcctgg acgccgggca ccagcgtgtt gccggtgtat
     3661 acgcacccgt ccaggtcgaa aatgaaagcc cgcagggatc tgaggtccat tgtggctccg
     3721 tgcctgcttc aaaaattccg tcgtcattcc ggcgaaagcc ggaatccagt gtcccgagga
     3781 tcctggaccc cggcgtccgc cggggtgacg atctgcggcg agagcctga
//
LOCUS       DBNGZC010000837         3179 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1834069, whole genome shotgun sequence.
ACCESSION   DBNGZC010000837 DBNGZC010000000
VERSION     DBNGZC010000837.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1834069"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="AC16G0_14390"
     CDS             <1..390
                     /locus_tag="AC16G0_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5100839.1"
                     /translation="LVLFGIVKKNSILQIDHIIGLRAEGMPRAQAIIQANRDRLRPIL
                     MTTFAFVAGMLPLLMGSGSGAGTNRAIGSVIAGGQTLALLLTLLATPVAYSLFDDLAA
                     KFAPRAWLARLWHKGSPAVAIPEARES"
     gene            561..1061
                     /locus_tag="AC16G0_14395"
     CDS             561..1061
                     /locus_tag="AC16G0_14395"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011141970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5100840.1"
                     /translation="MRPERLAERAAIRQVNTLAFGRPNEAALVDALRKRGRPYLSLVA
                     IQDGRIVGHIFFSPVILESEGGNFPIFGLAPMAVLPECQRRGIGSRLVWRGLEECARI
                     GQTVVVVLGHPGFYPRFGFVPASRKGLYCEYAVPDDAFMAAELLPGALQGRTGLVRYL
                     PEFSEV"
     gene            complement(1067..2227)
                     /locus_tag="AC16G0_14400"
     CDS             complement(1067..2227)
                     /locus_tag="AC16G0_14400"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5100841.1"
                     /translation="MAEGPLFTRPFVLICLATFFFYLSFCLILPVMPLYVAGLGGTPT
                     QIGLLIGLFAFMSMLLRPPTGWIIDRRGNMPVLLAGMAIFLLASVGYALTRSVAAILA
                     LRAFHGVGMGLFPTAATVVVAELAPLSRRGEAMGWFGIANSIGLVLGPVVGTAVAGGM
                     GYSMLFFVAGGTALLGLVCILMLPPVERPSGWTGSLHWRDFFSRAAVLPSVLLLFLCL
                     PYGMMTAFIPIIGAGRGLGNPGTIYTVFALTIVLVRAKAGRVSDRRGRAVVIIPGMLA
                     GALGFVVFGLTAGPVGVLVGAAIYGACFGSVQPALLALTTDRVPPAERGKAMGTFYTA
                     WELGISAGATGSGMLLSVVDFPLLLLVGSVFPIAGALLALRARTPSVPPRTT"
     gene            complement(2220..>3179)
                     /locus_tag="AC16G0_14405"
     CDS             complement(2220..>3179)
                     /locus_tag="AC16G0_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor-dependent isomerase,
                     group 22"
                     /protein_id="HIR5100842.1"
                     /translation="GVTPESMGVPILSSMETVEIGRMSWGLPVLVDRNAHQADHIVLV
                     NRVKPHTNFRCHVESGLMKMLVIGLGKHQGALLAHRAAVDVGLDRMVPEVARYSLSKL
                     PILFGVGMVENVRHQTARVQAMLPEALEETEARLLEEAWQLLGRIPFDFLHLLIVDQI
                     GKDVSGTGMDPNVIGRMYLFPNEEPKSPRYIRILVRDLTETTAGNAVGMGLADFATRR
                     LANKINFQYTYTNALTGLSPMRSKMPIIFETDREAIEGALKTIGLTDPPEAKLARIKN
                     TLDLEYLHVSEALLPEIRDRQDLELLEGPQEFRFSATGDLVDG"
BASE COUNT          563 a         1072 c         1043 g          501 t
ORIGIN      
        1 ctggtgctct tcggcatcgt gaaaaagaac tccatcctgc agatcgatca catcatcggc
       61 ctccgcgccg agggcatgcc gcgcgcccag gcgatcatcc aggccaaccg cgaccggctg
      121 cgccccatcc tcatgaccac cttcgcattc gtcgccggca tgctcccgct gctcatgggg
      181 agcggttccg gcgccggcac caaccgcgcc atcggctcgg tcatcgccgg cggacaaacc
      241 ctggccctcc tgctcaccct cctggcaacg ccggtggcct actcgctctt cgacgacctc
      301 gccgcaaagt tcgcccctcg ggcgtggctg gcgcggctat ggcacaaggg cagtcccgcg
      361 gttgcgatcc ccgaagcgcg ggaatcctga atcgaccccg gccgcgccgg tgttccgggg
      421 ggatgtcctc ccaggccatg aagcctctgg gagaaaggag ccgcatgttc aacccagtct
      481 ccctgtcgcc tggccattct ggtgaatcat ctcacgccgg tcggcggtgt gattcagaag
      541 cactggatcg tacgctcgtc gtgcggccag agcgactcgc ggagcgtgcc gcgatccggc
      601 aggtgaatac cctcgccttc ggccggccca acgaagcggc cctggttgac gccctgcgca
      661 agcgtggccg gccatacctg tccctggtgg ccatccagga cgggcgcatc gtgggccaca
      721 tcttcttcag cccggtgatc ctcgaatccg agggggggaa cttcccgatc tttggccttg
      781 cacccatggc ggtgctgccc gagtgccagc gacggggcat cggttcgcgg cttgtctggc
      841 ggggtctgga ggagtgcgcg cggatcggcc agacggtggt ggtggtgctc gggcacccgg
      901 gcttctatcc tcgtttcggc ttcgtgcctg ccagccgaaa gggcctctac tgcgagtatg
      961 cggtccccga cgacgccttc atggcggcgg agcttctgcc tggagccctt caagggcgta
     1021 cgggcctcgt gagatacctg ccggaattct ccgaagtgta gctgcttcag gtggttcggg
     1081 gtggcacgga aggtgtccgc gcccggaggg ccagcagggc gccggcgatg ggaaagacgg
     1141 aacccaccag gagcaacagc ggaaaatcga caacgctgag gagcatcccc gatcccgtgg
     1201 cgcccgcgct gatgccgagt tcccacgcgg tgtaaaacgt ccccatggcc ttcccgcgct
     1261 ccgcaggtgg gacccgatcg gtggtgagcg ccaggagggc aggttgtacg ctgccgaagc
     1321 atgcgccgta gatcgccgca cccacgagca cgcccaccgg acccgcagtc agcccgaaga
     1381 ccacaaaccc cagcgctccc gccagcattc ccggtatgat cacgacggcc cgaccccgcc
     1441 gatccgagac tcgacctgcc ttggcgcgga ccaggacgat ggtcagggcg aagacggtgt
     1501 agattgtgcc gggatttccc aggccccggc cggcgccgat gatggggatg aaggccgtca
     1561 tcatcccgta cggcaggcag aggaagagca gcaggacgga gggcagcaca gccgcccggc
     1621 tgaagaaatc ccgccagtgc aggcttcccg tccatcccga cggccgttcg accgggggca
     1681 gcatcagaat gcacacgagc cccagcaggg ccgtcccgcc ggccacgaaa aacagcattg
     1741 aatagcccat cccaccggcc acggccgtcc cgaccaccgg accgagaacc aggccgatgc
     1801 tgttggcaat gccgaaccag cccatggcct cccctcgcct cgagagaggc gccagctccg
     1861 ccacgaccac cgttgccgcc gtggggaaga gacccatccc gacgccgtgg aacgcgcgca
     1921 gcgccaggat cgccgcgacc gatcgggtca gcgcatatcc caccgatgcc aggagaaaga
     1981 tcgccatgcc cgccaggagg accggcatgt tcccgcggcg gtcaatgatc catccggtgg
     2041 gagggcggag cagcatggac atgaaggcga agaggccgat gaggaggccg atctgggtcg
     2101 gggtcccgcc caggcccgcg acatacaggg gcatcaccgg gaggatgagg cagaaactca
     2161 gatagaagaa gaaggtggcc aggcagatga gaacaaaggg ccgggtgaag aggggaccct
     2221 cagccatcca ccagatcgcc cgtcgccgag aaccggaact cctggggacc ttcgagcagc
     2281 tccagatctt gccggtcccg gatctccggg agcagggcct cggagacgtg gaggtattcc
     2341 aggtccagcg tgttcttgat acgcgccagc ttggcctccg gcggatcggt gaggccgatg
     2401 gtcttcaggg cgccctcgat ggcctcacgg tccgtctcga agatgatggg catcttgctc
     2461 cgcatgggag agagaccggt cagggcgttg gtgtaggtgt actggaagtt gatcttgttg
     2521 gccagccgcc gcgtggcgaa gtccgccagg cccattccca cggcgttgcc ggccgtggtc
     2581 tcggtgaggt cccgtaccag gatccgaatg tagcgggggc tcttgggctc ctcgttggga
     2641 aagaggtaca tccggccgat gacgttggga tccatgcctg tcccgctgac atccttcccg
     2701 atctgatcca cgatgagcag gtgcaggaag tcgaagggga tgcgccccag aagctgccag
     2761 gcctcctcga gcagccgggc ttccgtctcc tccagggcct caggcagcat ggcctgcact
     2821 cgggccgtct gatgccggac gttctccacc atgccgactc cgaacaggat ggggagcttg
     2881 gagagcgagt agcgggcgac ctccgggacc atgcgatcca ggcccacgtc caccgccgcc
     2941 cggtgcgcca ggagggcgcc ctggtgcttt cccaggccga tgaccagcat cttcatcagt
     3001 ccgctctcca cgtggcagcg gaagttggtg tggggcttca cccggttcac caggacgatg
     3061 tgatccgcct ggtgggcgtt cctgtccacc aggaccggca gcccccagga catccgcccg
     3121 atctccaccg tctccatgga ggacaggatg ggcacaccca tgctctcggg cgtcacccc
//
LOCUS       DBNGZC010000838         2923 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1733226, whole genome shotgun sequence.
ACCESSION   DBNGZC010000838 DBNGZC010000000
VERSION     DBNGZC010000838.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1733226"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="AC16G0_14410"
     CDS             <1..431
                     /locus_tag="AC16G0_14410"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR5100843.1"
                     /translation="RRLGCLGHSLGGQETLFLSATDRRIAAAVSSCGFSSYQAIFDAA
                     ILHNLALYVPGLLAQGDLDRVLGLVAPRPFLALVGSGDPIFPLEGVQATVRGAKRAYA
                     RSKNRLGLEIFPVGHDFSAPMREAAYAWLDRWLRPEGRQP"
     gene            580..1200
                     /locus_tag="AC16G0_14415"
     CDS             580..1200
                     /locus_tag="AC16G0_14415"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR5100844.1"
                     /translation="MGSACEIRLILSDFGGVICTFDYRIFCERLAQRTGRTADQIYTA
                     VFTGPLQGDFESGKLSGRAYHRAVMARLGADVTYEEFFPMYGDIFTEIPATCELLRRL
                     HGRYPLYLLSDTNEIHFGYVRETVEVLSLFDQFVVSYEVGAMKPDPRIYQEALRRSGL
                     PASACVFVDDREANVLGALRVGIPALRFESADRLAADLASLGVVIP"
     gene            1197..2111
                     /locus_tag="AC16G0_14420"
     CDS             1197..2111
                     /locus_tag="AC16G0_14420"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5100845.1"
                     /translation="MTAGIKVLDGHMHLLTTETEREGRDWLPSPALAAASQRRRERYE
                     RELAIPSAVPAEETVQVAASRWLADFDRYGVAAAVFLALSPRPETLRRFTLQRPDRLF
                     AFTFLNPWEPDAAEILEGDVRQRGFRGLKLYPSIQAFHVWDERAYPVYERAEALGIPI
                     LCHFGVTLDYRSDLRYSNPLDLHPVARDFPGLNFIVAHAGAGFFRETLFLAYHCANVC
                     VDISGSGTWMGYLPRPFTRQQVLERLLAVFGPARILFGTDSRHASEGYRHWLLTEHRH
                     ILETLGIPEEDQRDILGGNMARLLGISW"
     gene            2116..>2923
                     /locus_tag="AC16G0_14425"
     CDS             2116..>2923
                     /locus_tag="AC16G0_14425"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR5100846.1"
                     /translation="MGDLDVVAVGELNPDLILDGLPGPPILGREILASRATFTLGSST
                     ALCAANLAALGLKVGIVGKVGDDPLGDFVIRALGDRRIETGRVVRDAAVGTGVTVSLA
                     YPEDRAMLTYLGAMAQLMPGEVDLDYLASARHLHVSSLFLQRALQPGCLEIFRAARKR
                     GLTISLDPGWDPEEKWNAGIAALYPWLGVLFVNEAEVCALAAQAEWQGAAAALANRVE
                     TLVVKRGPAGAVAFRQGRMWQHPGFPVEAVDPTGAGDAFNAGFLFGWLAGW"
BASE COUNT          442 a          915 c          995 g          571 t
ORIGIN      
        1 ggcgcaggct gggatgcctg gggcattcac tgggggggca ggagactctc ttcctcagcg
       61 cgacggatcg acgcatcgcg gccgccgtgt cgagctgcgg attcagcagc taccaggcga
      121 tcttcgatgc tgccatcctg cacaacctgg ccctctacgt ccccggcctc ctggcccagg
      181 gggacctgga tcgggtgctg ggtctggtgg cgccgcgtcc attcctggcc ctggtcggaa
      241 gcggcgatcc catatttcct ctggagggag tccaggccac ggttcgtggg gcgaagcgcg
      301 cctacgccag gtccaagaat cgcctggggc tggagatctt cccggtgggt catgacttct
      361 ccgcccccat gcgtgaggcg gcctatgcct ggctggaccg atggctccgc cccgaggggc
      421 ggcagccttg accgccaagg cgccaaggac gccaagaatg aatcatgctc tcagacagaa
      481 gaccgggaac ggctaagagg cgacggcacc ggttttccgt ctcaggtaga taaggacatc
      541 cagtgctctt cttggcgacc ttggcgtctt ggcggtgaaa tgggatcagc gtgcgagatc
      601 cggctgatcc tcagcgactt tggcggggtc atctgcacct ttgactaccg gattttctgc
      661 gagcggctgg cccagcggac cggccggact gccgatcaga tctataccgc tgtcttcacc
      721 ggccccctcc aaggagactt cgaaagtggg aagctttcag gccgggccta ccatcgagcg
      781 gtgatggctc ggctgggtgc agacgtcacc tatgaagagt tcttccccat gtacggggac
      841 atcttcaccg agatcccggc gacgtgcgag ttgcttcgcc ggctgcacgg ccggtatccc
      901 ctctacctcc tctcggacac caacgagatt cacttcggct acgtgcgaga gacggtggag
      961 gtgctgagtc tcttcgatca gttcgtcgtc tcctacgagg tcggggccat gaagccggat
     1021 ccgcggatct accaggaggc cctccggcgg tcggggctgc cggcttcggc ctgcgtcttc
     1081 gtggacgatc gtgaagccaa cgttttgggc gccctacgcg tggggattcc tgcgctgcgg
     1141 ttcgagtcgg cggacaggct cgccgccgac ctggccagtc tgggggtggt catcccgtga
     1201 cggccggcat caaggtgctg gacggacata tgcatctcct gacgaccgag acggagcggg
     1261 agggtcgtga ctggctgccg tccccggccc tggcggcggc cagccagagg cggcgagagc
     1321 gatacgagcg tgagctggcc atcccttccg ccgtgcctgc cgaggagacg gtccaagtcg
     1381 cggcgtcgcg gtggctggcg gactttgatc gctacggcgt tgcggccgcg gtattcctgg
     1441 cgctctcacc caggccggag acgctccggc gtttcaccct ccagcggccc gatcggctct
     1501 tcgccttcac cttcctgaac ccctgggaac cggatgcggc cgaaattctg gagggggatg
     1561 tccggcagcg agggtttcgc ggtctgaagc tctatccctc gatacaggct ttccacgtgt
     1621 gggatgagcg ggcctatccc gtgtacgaac gcgccgaggc gctcggaatc ccgatccttt
     1681 gccatttcgg cgtgaccctc gactaccgct cggacctccg ctatagcaat cccctggacc
     1741 tgcatcccgt ggcccgagac ttccccggcc tcaatttcat cgtcgcgcac gccggggccg
     1801 gctttttccg ggagaccctg tttctcgcct atcattgcgc caacgtctgc gtggatatct
     1861 ccggttcggg gacctggatg ggctacctcc cgagaccgtt cacacgtcag caggtgctgg
     1921 agcggctcct ggcggtcttc ggtccggccc gaatcctgtt cggtaccgat tcgcggcacg
     1981 cctccgaggg ataccgccac tggctgctca cggagcatcg ccacattctg gagacgcttg
     2041 gcatccctga ggaggatcag cgtgatattc tcggaggaaa catggcgcgt ctcctcggaa
     2101 tctcctggtg agggtatggg cgacctggat gtggtggccg tcggggagct gaatccagat
     2161 ctgatcttgg acgggttgcc tggcccgccg attctgggaa gggagattct ggcaagccga
     2221 gcgactttca ccctggggag ctcgacggct ctgtgcgccg caaacttggc ggccctgggt
     2281 ctcaaggtgg gaatcgtagg aaaagtaggg gacgaccccc tcggcgattt cgtcatccga
     2341 gcgcttggcg atcggcggat cgagaccggc cgggtggttc gcgatgcggc tgttgggact
     2401 ggcgtgacgg tgtcgctggc ctatccggag gaccgggcca tgctgacgta cctcggagcc
     2461 atggcgcagc ttatgcccgg ggaggtggac ctggactatc tggcatctgc gaggcacctg
     2521 catgtctcgt cgctctttct ccagcgagcc ctccagccgg ggtgcctcga aatatttcgt
     2581 gccgccagga agcgcggcct cacgatttcc ttggatcccg gctgggatcc ggaggagaaa
     2641 tggaatgccg ggatcgcggc gctgtacccg tggctcggtg tcctgttcgt caacgaggcg
     2701 gaggtctgtg ccctggcggc ccaggcggag tggcaggggg cggcggcagc cctcgcgaat
     2761 cgcgtggaga cgctcgtggt caagcgcggg ccagccggcg ccgtcgcctt ccgccagggg
     2821 aggatgtggc agcatccggg attccccgtg gaggctgtgg acccgacggg cgcgggcgat
     2881 gctttcaacg ccggcttcct gttcgggtgg ctcgccggct ggc
//
LOCUS       DBNGZC010000839         6768 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2325014, whole genome shotgun sequence.
ACCESSION   DBNGZC010000839 DBNGZC010000000
VERSION     DBNGZC010000839.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6768)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2325014"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..956
                     /gene="tsf"
                     /locus_tag="AC16G0_14430"
     CDS             48..956
                     /gene="tsf"
                     /locus_tag="AC16G0_14430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HIR5100847.1"
                     /translation="MTISATMVRDLREKTGAGIMECKAALAETSGDLEKAIEVLRKRG
                     LKVAEKKAGRTAADGLVVAWTSPGKDVGALVEINCETDFVARTEQFAGLTRSLVALVG
                     ADTAAADVAILLERSLNGKRVAEVVKETIGSLGENVVIRRAARLALPSGAKGLVTSYL
                     HAGGKIGVLVEVRCASEGVAKGEGLAQLAKDLALQVCSAEPAYLTRDQVPAEILDRER
                     DILRAQPDLQGKPAAIQDKMIQGRLEKFYTERCLVDQVFIRDPEGKQKIRDVLKAAEK
                     KLGEPVTVATFARFRLGEGVEKRAAE"
     gene            1069..1788
                     /gene="pyrH"
                     /locus_tag="AC16G0_14435"
     CDS             1069..1788
                     /gene="pyrH"
                     /locus_tag="AC16G0_14435"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR5100848.1"
                     /translation="MADLKYRRILLKVSGEALAGDLEYGISTEVVSFIAEEIREVHRL
                     GVQIGVVIGGGNIFRGVEASARGMDRSSADYMGMLATVINGLALQDALEKHQVDTRVQ
                     TAIEMREVAEPFIRRRALRHLEKGRVVIFVGGTGNPYFTTDTAASLRAMEIGADVVFK
                     ATKVDGVYSADPKIDPTASKFDELTYLEVLSRRLKVMDSTAISLCMDNGFPIVVFNLN
                     NKGCLRQLVFGERVGTLVRGG"
     gene            1795..2349
                     /gene="frr"
                     /locus_tag="AC16G0_14440"
     CDS             1795..2349
                     /gene="frr"
                     /locus_tag="AC16G0_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012447887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR5100849.1"
                     /translation="MESVLRKAEQDMKRATEAMVKEFNSVRTGRASVALLDGIHVDYH
                     GSSTPLNQVAGLAVPERRLITIQPWEQSMVPVIEKAILKSDLGITPNSDGKVIRLAVP
                     ALTEERRRELVKVVHKIAEEGRVVIRNVRRDANEALKKLEKNKQVSEDGHRKGMEKIQ
                     DLTNRMIKSVDELLAKKEKEIMEF"
     gene            2374..3186
                     /locus_tag="AC16G0_14445"
     CDS             2374..3186
                     /locus_tag="AC16G0_14445"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR5100850.1"
                     /translation="MADGGIMHRQRILSALLLIPLFLLLVQFGSLIHFTLLVGLAVGL
                     AAWEFSRLCPLGADPWLSGLSVLGALIWYAATLYTGNLAGILALLSAVALLRAILVRG
                     EFRVNVQQAAWMILGVAYVGGLLSFPSLLRGLNDGRQYVYFLAFTTWAGDTGAYYVGS
                     RLGRRPLCPGISPKKTVEGAVGGIIATVILALAGSAWIWPNIPWGRAAVAGLVLAVVG
                     IAGDLCESAVKRAASAKDSGGIIPGHGGVLDRLDSLMFTGPVLFALTWIGWV"
     gene            3168..4778
                     /gene="lnt"
                     /locus_tag="AC16G0_14450"
     CDS             3168..4778
                     /gene="lnt"
                     /locus_tag="AC16G0_14450"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HIR5100851.1"
                     /translation="MDRLGVIAERAPLALDLPRPVRLGLAVGSGLLCSAAFPSWDIGA
                     LAFVGLVPLFLAVEGAPPRQAAWLGYVSGLTFYLATIWWVINTMTTYGRMPLALSLVT
                     LLLLCGVLAGYTAAFTWLLSLGERRLRLPGGLLPLTAAGLWTALELLRTYLFSGFPWA
                     LLGYSQYRQPTIRLLAAAVGVYGISALLVLVNGALADVVIRWLRPPIEQGRFRGALLS
                     VGAACLALAATLGYARAIWHDPTGGAPIRVGLLQGNIDQSVKWERSYQAKTLDIYERL
                     AQRAAAEQPALIVWPESAVPFFLRYEPELGLRVRRFAVETGIPMLVGSPDVREDQRLY
                     NSAFLLGRDGQIHGRYDKRHLVPFGEYVPLKRIFFFLDKLVVGIGDFGSGRTATVFSL
                     DGIRFGVMICYEAIFPAEVRQFARQGAEFLVNITNDAWFGRSGAPSQHLAMAAMRAVE
                     NGSYLVRAANTGITAVIAPTGEILAHTGIFMEAELVGTIRPRLEETPYTQYGDVLPWA
                     CLVFLGAYLLAVGRARWLQGAAGTASGG"
     gene            4795..5902
                     /gene="prfB"
                     /locus_tag="AC16G0_14455"
     CDS             join(4795..4863,4865..5902)
                     /gene="prfB"
                     /locus_tag="AC16G0_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157471875.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR5100852.1"
                     /translation="MIPDLQRNVDELQRKLEQLRVYLDVPGKTARLQTLESVTAAPEF
                     WGDQEKAQRVLKEQSDLRQTLEQHTGLVKELDEQRMLLELAADEEDVETLQEVSTGLQ
                     ELGRQVRTLELQTVLTGQYVKGNAIIAINAGAGGTESQDWAQMLFRMYLRWAETKRFK
                     TEVIDILEGDEAGIKNVTFTVEGNYAYGYLRAEIGVHRLVRISPFDANKRRHTSFASV
                     FVFPEISDDIDIKLDEKDLRIDTYRAGGHGGQHINVTDSAVRITHLPTGIVAQCQNER
                     SQHKNKAMAMKVLRSRLYDHYRAQKDEEMAKLEGVKQAIAWGSQIRSYVLAPYQLVKD
                     HRTGEETGNVTAVLDGDVDRFIEAYLFKPPEQKA"
     gene            6017..6259
                     /locus_tag="AC16G0_14460"
     CDS             6017..6259
                     /locus_tag="AC16G0_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004176061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100853.1"
                     /translation="MPGEDMQAELERLRAENEALKAKTKGSTSIRVSEKGGVSVYGLG
                     RFPVTLYKEQWLKLLAMAEDIKRFIAENESRLKTKD"
     gene            complement(6324..6662)
                     /locus_tag="AC16G0_14465"
     CDS             complement(6324..6662)
                     /locus_tag="AC16G0_14465"
                     /inference="COORDINATES: protein motif:HMM:NF020967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative signal transducing protein"
                     /protein_id="HIR5100854.1"
                     /translation="MARERARCPRCTQTYPHGTTVCPACHVALELPGSERDGPPAILI
                     FETWDRPSADIVAGLLESNGVTCLLRGTGDATHFGIGPQGFWRVYVQAADEPRAQEIL
                     DAEIGREEAE"
BASE COUNT         1189 a         2042 c         2387 g         1150 t
ORIGIN      
        1 ggggcgggcg ccgcaacggc cgaggcgagc agccagaagg agtccgcatg acgatctcgg
       61 caacgatggt tcgcgatctt cgcgagaaga cgggcgcggg gatcatggaa tgtaaggcgg
      121 ccctggcgga aaccagcggc gacctggaaa aggcgatcga ggtcctgcga aagcgcgggt
      181 tgaaggtcgc cgagaagaag gcgggccgga ccgcggccga cggcttggtg gtggcgtgga
      241 cctcgccagg gaaggatgtg ggggcgctgg tcgagataaa ctgcgagacg gacttcgtgg
      301 cccgcacgga gcagttcgcg ggtctcaccc gttcgctggt cgccctggtg ggtgcggaca
      361 ccgccgcggc ggatgtggcc atcctgctgg agcggtccct gaatggcaag cgagtggccg
      421 aggtggtcaa ggagaccatc ggttccctcg gagagaacgt ggtgatccgc cgggccgcca
      481 gactcgcctt gccgtcgggc gccaagggtc tggtcacgag ctacctccac gccggaggca
      541 agatcggcgt cctggtggag gtccgttgcg cgtccgaggg ggtggccaag ggtgagggac
      601 tggcccaact ggcgaaggac ctagcgcttc aggtgtgctc cgccgagccg gcctacctga
      661 cgcgcgatca ggtcccggcc gagattctgg atcgggagcg agacatcctg cgcgcccagc
      721 ccgaccttca gggcaagccg gcggcgatcc aggacaagat gatccaggga cgcctggaga
      781 agttctacac ggagcgctgc ctggtggacc aggtcttcat ccgggacccc gaagggaagc
      841 agaagatccg agacgttctg aaggcggccg agaagaagct gggagagccg gtgaccgtgg
      901 cgaccttcgc ccggttccgt ctgggtgaag gcgtcgaaaa gcgggccgcg gagtagagtt
      961 ctggccggtc cgtttggccg atctggatcc ggtcctctcc agttgacgca cccgggttgc
     1021 agactgccgc gttgccaaat gcgtggcagc cccggagagg tggggagcat ggcggaccta
     1081 aaataccggc gcatcctcct gaaggtcagc ggcgaagccc tggcgggtga cctggagtac
     1141 ggcatcagca ccgaggtcgt cagcttcatc gcggaggaga tccgggaggt ccaccgtttg
     1201 ggcgtccaga taggcgtggt gatcggcggc gggaacatct tccgcggggt cgaggccagc
     1261 gctcgcggga tggatcggtc ctcggccgat tacatgggga tgctggccac ggtcatcaat
     1321 gggttggccc tccaggacgc gctggagaag catcaggtgg acacgcgggt ccagaccgcc
     1381 atcgagatgc gcgaggtggc cgagccattc attcggcggc gggctttgcg ccacctggag
     1441 aaggggcggg tggtgatctt tgtgggcggc acggggaacc cgtacttcac cacggacacc
     1501 gctgcatccc tgcgggccat ggagatcggg gcggatgtgg tgttcaaggc gacgaaggtg
     1561 gacggggtgt acagcgccga tccgaagatc gatcccacag cctcgaagtt cgacgagctg
     1621 acctacctgg aggtgttgag ccggcgcttg aaggtcatgg actccacggc catctcgctg
     1681 tgcatggaca acggattccc catcgtggtc ttcaacctga acaacaaggg atgcctgcgc
     1741 cagctcgtct tcggtgagcg ggtgggcacc ctggtcaggg gaggctgagg cagcatggaa
     1801 agcgtgctcc gcaaggccga acaagacatg aagcgggcta ccgaggccat ggtgaaggag
     1861 ttcaacagcg tccggacagg ccgcgcctcg gtggcactcc tggatggcat ccacgtggac
     1921 taccacggga gttccacgcc cctgaatcag gtggccgggc tcgcggtgcc ggagcgacgg
     1981 ctcatcacga tccagccctg ggagcagtcc atggtgccgg tcatcgagaa ggccatcctg
     2041 aagtccgatc tggggatcac ccccaatagc gacgggaagg tgatccgcct ggcggtcccg
     2101 gccctcaccg aggagcgccg ccgcgagctg gtgaaggtcg tgcacaagat cgcagaggaa
     2161 ggccgggtgg tcatccgcaa cgttcggcgc gacgccaacg aggccctgaa gaagctggag
     2221 aagaacaagc aggtgtccga ggacgggcat cgcaagggca tggagaagat ccaggacctg
     2281 acgaaccgca tgatcaagag cgtggacgag ctcctggcga agaaggaaaa ggaaatcatg
     2341 gaattctgat tccgcccggc cccccggtgc gggatggcgg acggcggaat catgcaccgt
     2401 cagcggatcc tcagcgcgct tctgctcatc cctctgttcc tcctgctggt tcaatttggt
     2461 tcgctgattc acttcacgct cctggtcggc ctggccgtcg gcctggcggc ctgggagttc
     2521 tcccggctgt gtcctctggg ggcggatccg tggctctcgg gtctcagcgt gctcggggcg
     2581 ctcatatggt atgcggccac gctctatacc ggcaatctgg caggcatcct ggcactgctc
     2641 tcggccgtgg cgctgctccg cgccatcctg gtccgggggg agtttcgggt gaacgtccag
     2701 caggctgcct ggatgatctt gggggtggca tacgtgggag gtctgctgag ttttcccagt
     2761 ctgcttcgcg gcctcaatga cggccggcag tatgtttact tcctggcctt cacgacctgg
     2821 gccggggata ccggggctta ctacgtggga tcgcgcttgg gtcgccggcc gctctgccca
     2881 gggatcagtc ccaagaagac ggtcgaggga gccgtgggtg gaataatcgc cacggtgatc
     2941 ctggctctcg ccgggagcgc gtggatttgg ccgaatattc cgtggggtcg ggccgcggtc
     3001 gcggggctgg tgctggcggt cgttggtatc gccggcgacc tgtgcgagtc ggccgtgaaa
     3061 cgcgctgcct cggccaagga tagcggaggc atcatccccg gacacggcgg ggtcctggac
     3121 agactggaca gcctgatgtt cacggggccg gtgctcttcg ccctgacatg gatcggctgg
     3181 gtgtgatcgc cgagcgggcg cccctggccc tggatctgcc gcggccagtg cggctgggat
     3241 tggcggtggg aagcggcctg ctctgcagtg cggccttccc ctcctgggac atcggcgcgc
     3301 tcgccttcgt cggcctggtc ccgctctttc tggcggtcga gggggcgccg ccgcgccagg
     3361 ccgcctggct cggctatgtc tccgggctga ctttctatct ggcgaccatc tggtgggtca
     3421 tcaacaccat gaccacctac ggccggatgc ccctggcgtt gagcctggtg acgctcctcc
     3481 tgctctgcgg tgtcttggct ggatacacgg cggccttcac ctggctcctg tccctgggtg
     3541 agcgtcggct ccgacttccc gggggacttc tccccctgac cgccgcggga ctgtggacgg
     3601 ccctggaact cctccggacc tatctgttct cggggttccc ctgggccctg ctgggataca
     3661 gccagtaccg tcagcccacg atccgtctcc tggcggccgc cgtgggggtg tacggaatct
     3721 ctgcgctcct ggttctggtg aacggggccc tggcggatgt cgtgatccgc tggctgcgcc
     3781 ccccgatcga acaggggcgt ttccgggggg cgctcctgtc cgtgggggcg gcgtgtctcg
     3841 cgctggcagc cacgctcggc tatgcgcgag ccatctggca cgaccccacg gggggcgccc
     3901 cgatacgggt gggcctgctc caggggaaca tcgaccagtc ggtgaagtgg gagcgcagct
     3961 accaggcaaa gaccctggac atctacgagc gactggcgca gcgggcggcg gcggaacagc
     4021 cggccctgat cgtctggccg gagtcggcgg tcccgttctt cctgcgatac gaacccgaac
     4081 tcggcctgcg cgtccgccga ttcgcggtgg agacggggat ccccatgctg gtcgggagcc
     4141 cggacgtcag ggaggatcag cgattgtaca attctgcctt cctcttggga cgtgacgggc
     4201 agattcacgg acggtacgac aagcggcacc tggtgccctt cggcgagtac gtgccgctca
     4261 agcgcatctt cttcttcctg gacaagctgg tggtcgggat cggggacttc gggagcggtc
     4321 ggaccgccac cgtgttctcg ctggatggga tccgattcgg ggtcatgatc tgctacgagg
     4381 caatcttccc ggcagaagtt cgccagttcg cacgccaggg ggcagagttt ctggtgaata
     4441 tcaccaacga cgcgtggttc ggacggagcg gcgcgccttc gcagcatctg gcgatggcgg
     4501 ccatgcgtgc cgtggagaac gggagctatc tggtccgggc ggccaacacc gggatcaccg
     4561 cggtgatcgc cccgacggga gagatcctgg cgcacaccgg gatcttcatg gaagcggaac
     4621 tcgtagggac gattcgcccg cggctcgagg agaccccgta cacacaatat ggggatgtcc
     4681 tcccctgggc gtgtctggta ttcttgggtg cgtatctgct ggccgtcggc cgggcccggt
     4741 ggctgcaggg ggcggcgggc accgccagcg gcggataatt cgaaggaggc ggcaatgatt
     4801 ccggatcttc agcgcaacgt ggacgagctg caacgaaagc tggaacagct ccgggtgtat
     4861 ctttgacgtg cctggaaaga ctgcccggct ccagacgctg gaatcggtca cggcggcccc
     4921 ggaattctgg ggagaccagg agaaggccca gagggttctg aaggagcaga gcgacctccg
     4981 ccagaccctg gagcagcaca ccggcctggt gaaggagttg gacgagcagc ggatgctcct
     5041 ggagctggca gcggacgagg aggatgtcga gacgctccaa gaggtgtcga ccggtctcca
     5101 ggagcttggg cgccaggtcc gaaccctgga gcttcagacc gtcctcacgg gccagtacgt
     5161 gaagggaaac gccatcattg ccatcaatgc gggcgccgga ggcaccgagt cgcaggactg
     5221 ggcgcagatg ctgttccgca tgtacctgcg ctgggcggag accaagcggt tcaagacgga
     5281 ggtcatcgac atcctggagg gggacgaggc ggggatcaag aacgtcacct tcaccgtgga
     5341 ggggaattac gcctacgggt acctccgggc cgagatcggc gtccaccggt tggtgcgcat
     5401 ctctcccttc gacgccaaca agcggcggca cacctccttc gcctcggtct tcgtgtttcc
     5461 cgagatctcc gacgacatcg acatcaagct cgacgaaaag gatctgcgca tcgacaccta
     5521 ccgggccggc ggccacggcg gacagcacat caacgtgacc gactcggcgg tccgcatcac
     5581 ccacctgccc accgggatcg tggcccagtg ccagaacgag cggtcacagc acaagaacaa
     5641 ggccatggcc atgaaggtcc tgcggtctcg tctctacgac cattaccggg cgcagaagga
     5701 cgaggaaatg gccaagctgg aaggggtgaa gcaggcgatc gcttggggca gtcagatccg
     5761 atcctacgtt ctggcgccct accagttggt gaaggaccac cggacgggcg aggaaacggg
     5821 gaacgtcact gcagtcttgg acggagacgt ggaccggttc atcgaagcgt atctcttcaa
     5881 gccgcccgag cagaaggcat aggccgagta tccggatgca ccgattcgat ccctgtccct
     5941 gtcgctgtct ctgtcggtct tcgcggaaag cgtagcacca gcatccgggt gagcagaccc
     6001 gggaaaggaa gacatcatgc caggcgagga tatgcaggcc gagctggagc gattgcgcgc
     6061 cgagaacgaa gcactcaaag ccaagacaaa ggggtccacc agtatccggg tgagcgagaa
     6121 gggcggcgtg tccgtgtacg gcctgggccg cttcccggtg accctctaca aggagcagtg
     6181 gctcaagctc ttggcgatgg ccgaggacat caagcgcttc atcgccgaga atgagtcccg
     6241 cctgaagacc aaggactgat ttgcccctgc ctcacggggt catgcgtcag ggatgtggag
     6301 gcgtgcgggg cgaattcccg ccgtcactcg gcctcctctc gcccgatctc cgcatccagg
     6361 atctcctggg cccggggctc gtccgcggcc tggacataca cacgccagaa gccctgtggg
     6421 ccgatgccga agtgggtcgc atcgccggtc ccacgcagga ggcaagtcac gccattcgac
     6481 tcaagtaacc ccgcgacgat gtccgcggac gggcgatccc aggtctcgaa gatcaggatg
     6541 gcaggagggc cgtcgcgttc cgagccgggc agttccagcg cgacatggca cgcagggcag
     6601 accgtggttc catgtgggta cgtttgggtg cagcgggggc aacgggccct ctctcgcgcc
     6661 atcggcgacc tcattccatc cgagaatagc ctctggcgta gggctcgggc tcgggccata
     6721 tgccatacgc tatacgccaa gagcaggccc cggcccaaag ctcagcgg
//
LOCUS       DBNGZC010000840         5980 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1834399, whole genome shotgun sequence.
ACCESSION   DBNGZC010000840 DBNGZC010000000
VERSION     DBNGZC010000840.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5980)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5980)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5980
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1834399"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="AC16G0_14470"
     CDS             <1..777
                     /locus_tag="AC16G0_14470"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR5100855.1"
                     /translation="GGALGGEKDVLEGMRVGSVHLSLIGLTLMPFLDVIWGPYVFRDN
                     DHAKKVLRGEIGQSWKDRVLKESGGLRLLDYVYFGPRQLTTKNTPARTPTQLKGLKIR
                     VMEAPIYLATWKALGASPVPMAWPEVFTGLQQGTIDAQENPYELIVSNSLFEVQKYVI
                     STEHVRPFRFLLMNDAAFNKMTKEQQKMFTDTWQEIARDIEQEYVSKEQEYIGILKSK
                     GMTVIQPDLETYRTATKDVWKEFMPKAWGPGVFERVQAVK"
     gene            898..1911
                     /locus_tag="AC16G0_14475"
     CDS             898..1911
                     /locus_tag="AC16G0_14475"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR5100856.1"
                     /translation="MWTMRRGWLVVWTLFFVAVWCMPAAAQTVLKVGSQYAAKSTSIL
                     AMEKFAEYVKEKTQGKYVVRLFPDGQLGPDREMQQSLKMGTLDLLHATNNAPTLMKEG
                     RNFEATAAPYVFRTQEEYRKFLQTPLHDEMAAALEPGGSHLVGYAGDRPPRALTTSTT
                     PVRVPADMKGMKIRVPGMKALMAFFQEVGANPTPMPYTELFTALKTGVVVGQDNGIDN
                     VESAGFYEVQKYYMKLDHARGVYMLYASTPKWKTWPESLKKVLIEACQVAAEENNKLL
                     AEYEKVAYKRLQDHGMTILDVDHPAFEEIGKRVWKKFDGDLWEKGYMDRVQATLEQIR
                     KTK"
     gene            1933..2412
                     /locus_tag="AC16G0_14480"
     CDS             1933..2412
                     /locus_tag="AC16G0_14480"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR5100857.1"
                     /translation="MTFWRVVDWLVDSLAAVLLLVMSLVLAVEVVFRYGLNRPLSWTL
                     EVSLFCFVWMVWLGANGQMRSERQIRIDIVETYAPKSIQRVLLPANTVLSLVFLVFVI
                     YYGVQVTESQMSAVYDILPFSRGILYAAAPVGGSLMLLTLGRVLVRQIRRYYGGRGA"
     gene            2430..3713
                     /locus_tag="AC16G0_14485"
     CDS             2430..3713
                     /locus_tag="AC16G0_14485"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR5100858.1"
                     /translation="MDLLLLFGSLVLLMFLGLPITFSMGISGVFFLLVTGQTEMLIMI
                     PNRMVMALDSYGLMAIPFFYLAGSLMLIGGLTLRLGAFARAMIGHVTGGLSLVSVICS
                     MIFAGVSGSCVADASAVGSVLIPAMKKDRYPADFSAALTASAATCGHIIPPSIPMVLI
                     GVMQEMPIGKLFIAGAIPGLLLGFSLMATAYVISRRRNYPKEERRSTLREMSRAFLDG
                     FLTLIMPGIIVGGIVFGVVTITETGVLASAYALVLGFLYREIRPDNFWAMAKETAKGV
                     SNLLIILASAGFFGYVVMNTGVGDQLVALILSISTNKWMVLTMLVIFLLIIGCGLDVL
                     AIIFIFVPVMYPLIQKVGINPYHFSAVFVLSLGIALLTPPVGVLLYLASDMAEAPLVG
                     VIKELTPFLLVEIAVMLLVTYVPVLSTWLPSLISY"
     gene            3726..4466
                     /locus_tag="AC16G0_14490"
     CDS             3726..4466
                     /locus_tag="AC16G0_14490"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HIR5100859.1"
                     /translation="MTDLVIKPLHVGTVGRDKAIFTYLRNMGERIEVPVLAWYVEGGG
                     RRILVDTGGHDPNEVQLHAPYRRTPAQDPVAALAALGVAPEEIDTIILTHLHWDHASN
                     THRFPKARILVQREELRYAAAPLPPHQWAYRVHPPVASHWERYVAIDGDLEVAKGVSV
                     HLTPGHTPGLQGVAVRTASRVYYIASDNVPLAEMWHATDRYGASHWPGSIHVDLEAYV
                     KSLKRIEGLGDVVLPSHDASVLEQREYR"
     gene            4848..5672
                     /locus_tag="AC16G0_14495"
     CDS             4848..5672
                     /locus_tag="AC16G0_14495"
                     /inference="COORDINATES: protein motif:HMM:NF024587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HIR5100860.1"
                     /translation="MTIVAISREMGSGGYEIGAAVAKALNFEYVNRQILLEAAHAYGV
                     PEAMLVDVVERRPSLWERFDEERNRYLSFLEAAYYTFAERGNLVTASRSGPFFVRDVR
                     HALKVRIMAPVEVRVRRVMAQEGLDQKAATAKVRAYDREMSGRIDYLFEVDWTRPENY
                     DLVINTSDDTWEFYTDLLVAAARHPRYQPTPESQQKVRDLSLAAQVRAAIAKNPETRH
                     LNVKVTAQAGRVVLMGVVFSPALLDAAADVAERVPGVVSVSCETVEIPRVYPGPIM"
BASE COUNT         1096 a         1862 c         1889 g         1133 t
ORIGIN      
        1 ggcggggccc tcgggggcga gaaggatgtc ctggaaggga tgcgcgtcgg gtccgttcac
       61 ctgagcctga tcggcctcac cctcatgcct ttcctcgacg tcatctgggg gccctacgtc
      121 ttccgggaca acgaccacgc caagaaggtg cttcgcggag agatcggcca gagctggaag
      181 gaccgggtgc tcaaggagag cggaggtctc cggctcctgg actacgtgta cttcggcccg
      241 cgccaactga ccaccaagaa cacgcctgcc cggactccga cccagttgaa ggggctcaag
      301 atccgggtca tggaggcccc catctacctg gccacgtgga aggcgctggg tgcgagcccg
      361 gttcccatgg cttggccgga ggtgttcacc ggactgcagc agggcaccat cgacgcccag
      421 gagaatccgt acgagctgat cgtctccaac agcctcttcg aggtccagaa gtacgtgatc
      481 tccaccgagc acgtgcgccc gttccgcttc ctgctcatga acgacgccgc cttcaacaag
      541 atgaccaagg agcagcagaa gatgttcacg gacacctggc aggagatcgc cagggacatc
      601 gagcaggagt acgtctccaa ggagcaggag tacatcggca tcctgaagag caagggcatg
      661 accgtcatcc agccggatct cgagacctac aggacggcca ccaaggacgt gtggaaggag
      721 ttcatgccca aggcgtgggg cccgggcgtg ttcgagaggg tgcaggcggt caagtagctc
      781 cccgtccgtg cccagcggcg cccggcggga tcgccgggca acaacgagtg gggcgtcagg
      841 cccgtgagaa ccagcttcac gcgatgtgat ttgggagacc cttcaaggga gggagccatg
      901 tggacaatgc gcaggggttg gctggtcgtt tggacgctct tcttcgtcgc cgtctggtgc
      961 atgcccgccg cggcccagac cgtcttgaag gtcggatcgc aatacgcggc caagtccacc
     1021 tccatcctgg cgatggagaa gtttgccgaa tacgtcaagg aaaagaccca ggggaaatac
     1081 gtcgtccgtc tctttcccga cggacaactg ggacccgacc gcgagatgca gcagtcgctg
     1141 aagatgggca cgctggactt gctgcacgcc accaacaatg cccccaccct catgaaggaa
     1201 ggcaggaact ttgaagccac ggcggcaccc tatgtcttcc ggacccagga agaatatcgg
     1261 aaattcctcc agacgccgct gcacgatgag atggcggctg ccctggagcc gggcggcagc
     1321 cacctggtgg gctatgccgg cgaccgtccc ccccgagcct tgaccaccag caccaccccg
     1381 gtccgggttc cggcggacat gaaggggatg aagatccggg tgccggggat gaaagctctc
     1441 atggccttct tccaggaggt cggcgccaat cccacgccca tgccctacac cgagctcttc
     1501 accgccctga agacgggcgt ggtggtggga caggataacg ggatcgataa cgtcgaatcc
     1561 gccggcttct atgaagtgca gaagtattac atgaagctgg atcacgcccg gggcgtctac
     1621 atgctctacg ccagcacgcc gaaatggaag acctggcccg agagtttgaa aaaggtcctg
     1681 atcgaggcat gccaggtggc cgccgaggag aacaacaaac tcctcgccga atacgagaag
     1741 gtcgcctaca agcgactgca ggatcacggc atgacgatcc tcgacgtcga ccaccccgcg
     1801 ttcgaggaga tcggcaagcg ggtgtggaag aagttcgacg gcgatctctg ggaaaaagga
     1861 tacatggatc gggtgcaggc cacgttggag cagatcagga aaaccaagta gcctttcaaa
     1921 ggaggcgccc ccgtgacgtt ctggagggtc gtcgactggc tcgtggactc actggccgcc
     1981 gtgttgctcc tggtcatgtc gctggtcctg gccgtggagg tcgtctttcg ctatggcctg
     2041 aatcggccgt tgtcctggac ccttgaggtc tcgctgttct gcttcgtgtg gatggtctgg
     2101 ctgggagcca acgggcaaat gcgaagtgag cggcagatcc ggatcgacat tgtggagacg
     2161 tacgccccga agtccatcca gagggtcctc ttgccagcga acacagtcct ttcactggtc
     2221 ttcctggtgt ttgtcatcta ctacggtgtt caggtgacgg aatcccagat gagcgcggta
     2281 tacgacatcc tgcccttctc ccgggggatt ctgtacgctg cggctccggt gggtggaagc
     2341 ctgatgctcc tcaccctggg aagagtcctg gtccgccaga ttcgacgcta ttacggaggc
     2401 cggggggcct gagagaaagg aacggccgta tggaccttct tctccttttc gggagcctcg
     2461 tcctcctcat gttcctgggc ctcccgatca ccttttccat ggggatctcc ggagtgttct
     2521 tcctcttggt caccggccag accgagatgt tgatcatgat ccccaaccgc atggtgatgg
     2581 ccctggacag ttatggcctg atggccatcc ccttcttcta ccttgcggga tccttgatgc
     2641 tgatcggagg gctcacgctg cggctggggg ccttcgcccg ggcgatgatc ggacatgtca
     2701 ccggggggct ctcactggtg agtgtcatct gcagcatgat cttcgccgga gtctcgggct
     2761 cctgtgtcgc ggacgcctcg gccgtcggca gcgtcctcat cccggccatg aagaaggacc
     2821 ggtatccggc agacttctcg gcagctctga ccgcatcggc ggcgacgtgc ggccatatca
     2881 tccctccgag cattcccatg gtcctgatcg gggtgatgca ggagatgccc atcggcaagc
     2941 tcttcattgc tggcgcgatc cccggcctcc tgctcggttt ttcgctgatg gcgacggcct
     3001 atgtcatcag tcgtcggagg aattacccca aagaggagcg gcgctcgact ctccgggaaa
     3061 tgagccgagc cttcctggat ggattcttga ccttgatcat gccggggatc atcgtcgggg
     3121 ggatcgtctt cggcgtggtc accatcacgg agacgggtgt cctggccagt gcctacgctt
     3181 tggtcctcgg gttcctctac cgggagatcc gccccgacaa tttctgggcc atggccaagg
     3241 agaccgccaa gggagtctcc aacctgctga tcatcctggc ctccgccggc ttcttcggct
     3301 acgtggtcat gaacaccggg gtaggagacc agctcgtcgc cttgatcctc tccatcagca
     3361 ccaacaagtg gatggtcctc acgatgctgg tgatcttcct gctgatcatc gggtgcgggt
     3421 tggacgtgtt ggcgattatc ttcatcttcg tccccgtcat gtacccgctc atacagaagg
     3481 tgggcatcaa cccgtatcat ttctctgcgg tgtttgtcct gtccctgggg atcgcgctgc
     3541 tgactccccc ggtcggggtc ttgctctatc tcgcttccga catggctgag gcgccgctgg
     3601 tcggggtgat caaagagttg actcctttcc tgttggtgga gattgccgtc atgctgctgg
     3661 tgacctatgt ccccgtcctc tccacatggc tgccgagtct gatttcatac taagaggaga
     3721 cgcccgtgac ggacctggtc atcaaacccc tgcatgtcgg cacggtcggt cgcgacaagg
     3781 cgatcttcac ctacctcagg aacatggggg agcggatcga ggtgcccgtg ctcgcctggt
     3841 acgtggaggg gggcgggcgt cgcatcctgg tggacacggg aggccacgac ccgaacgagg
     3901 tccagttgca cgccccgtat cgcagaacac ccgcccagga ccccgtggcc gccttggctg
     3961 cgctgggggt ggctcccgag gagatcgaca cgatcatcct cacgcacctg cattgggacc
     4021 atgcgtccaa cacccaccgc ttcccgaagg cccgcatcct cgtccagcgg gaggagcttc
     4081 ggtacgccgc ggctcccctg ccccctcacc aatgggcgta tcgcgttcac ccgccggtgg
     4141 cttcacactg ggaaagatac gtggcgatcg acggcgatct ggaggtggca aagggcgtat
     4201 cggttcacct gaccccgggg catacgcccg gccttcaggg ggtggccgtc cgcaccgcca
     4261 gccgggtgta ctacatcgcc agcgataacg tccccctggc cgagatgtgg catgccacgg
     4321 atcgatacgg tgcgtcccat tggccgggga gcattcatgt ggacctggaa gcctacgtca
     4381 agtcgctgaa gcggatcgag gggctggggg atgtagttct accaagccat gacgccagcg
     4441 tgctcgaaca gcgcgaatac cgctagccca cctccctcgg cgttttcggc gacggccttg
     4501 cacgccgggt aagccctcct ctgggccacg tgccaggctt tcccgtggtg cccacacccg
     4561 ctggcctcgc atgccatctc tactagtgca ctctgcccga aataccccca cataaaggga
     4621 gccccccttt ggcctcgccg cacgctacga gttgcgaagc cgcgcgcgcc cggagccggg
     4681 cctccaccgg caagttctag agtatccccg gcaacctgtc cggtaccttc tttacgaccg
     4741 cgtctcgcct cggcccacct tgaccgttga cggcgctgca cacccgtgct acgagagaag
     4801 ctgcgggatg cgtccccggg cagccgaaga acgaaggagg gagggtcatg accatcgtgg
     4861 caatttcccg agaaatgggg agcgggggct acgagatcgg cgccgccgtg gccaaggcgc
     4921 tgaacttcga gtacgtgaat cggcagatcc tccttgaggc cgcgcacgcc tacggggtgc
     4981 cggaagcgat gctcgtggat gtggtggagc gtcgcccgtc cctctgggaa cgcttcgatg
     5041 aggagaggaa tcgctatctc agcttcctgg aggcggccta ctataccttc gccgagaggg
     5101 ggaatctggt cacggccagt cgcagcggcc ctttcttcgt ccgggacgtg cggcacgccc
     5161 tcaaggtgag gatcatggct cccgtcgagg tgcgcgtgcg ccgggtcatg gcgcaggagg
     5221 ggctggacca gaaagccgcc acggccaagg tccgggccta cgaccgggag atgtccgggc
     5281 gcatcgacta cctcttcgag gtggactgga cccgacccga gaattacgat ctggtgatca
     5341 acaccagcga cgatacctgg gaattctaca cggacctttt ggtggcggcg gcccgccacc
     5401 cacgctacca gcccacgccg gagtcgcaac aaaaggtccg cgacctcagc ctcgcggcgc
     5461 aggtgcgcgc cgccatcgcc aagaacccgg agacccggca cctcaatgtc aaggtcaccg
     5521 cccaggcggg ccgcgtggtg ctgatgggcg tcgtcttcag cccggcctta ctggacgccg
     5581 cggccgatgt ggccgagcgc gtcccggggg tcgtcagcgt ctcctgcgag accgtggaga
     5641 tcccgcgggt ttacccggga ccgatcatgt gacgcgaact tcacgtcgcc ggtcgccgtc
     5701 tccgcctcgc tggcggatgg cggtgagaag ctcggggaga tgaaggcctg cgggcattag
     5761 ccccgatgcg aaaatcgctc caccacaaag tcaccaagac actaaggatg ggattggtca
     5821 tcaaggactt ggataccgaa aacccggaga ccccaagagt gcgcttgagg aatagcccgt
     5881 ctgggactgg aatgcagtct gtttctttgg ggcctttgtg gcttcgtggt gagacttata
     5941 cgcatcgagg tccgctctgt gtctacgtgc ctccgtggtg
//
LOCUS       DBNGZC010000841         3684 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2027245, whole genome shotgun sequence.
ACCESSION   DBNGZC010000841 DBNGZC010000000
VERSION     DBNGZC010000841.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2027245"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            464..2557
                     /gene="acs"
                     /locus_tag="AC16G0_14500"
     CDS             464..2557
                     /gene="acs"
                     /locus_tag="AC16G0_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0046356 - acetyl-CoA catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase alpha subunit"
                     /protein_id="HIR5100861.1"
                     /translation="MLDGFFKPTAVTVVGASREEGKVGYSIVLNLIRGKFTGPIYPVN
                     PKASEILGLKCYPDVTSVPADADLAVLVIPPKICIPTLETCAGRGIRSAIVISAGFKE
                     VGGEGAEIEKALRARVRELGIRVVGPNCLGIISTQSRMNATFAAGMPPEGGISFFSQS
                     GALCTAILDWAIGNNVGFAKFISLGNKADMSEVDFIQALAEDPETRVIVGYIEGVEDG
                     RRFMEVARAASRTKPFIIVKSGGTAAGARAASSHTGTLAGSEKSFNAAFRQSGVIRAE
                     SIEELFNYARAFADQPLPQGPSLCIVTNAGGPGIIAADAVERSTVKMASLSPETVERL
                     RATLPPTAALYNPVDVIGDAKEDRYKAALDAVAADAHVDGVLALATPQDMTEYDKFAE
                     VVGQAAKASSKPFFTAFMGEASLPKAREILRRHGVPQYPYPEPAVRTFEAMLRYRQWQ
                     EYTPTPPTEFHVDKGQVALTILSARRTRRTQLGETEAREIIAAYGFRLPQNVLARTVD
                     DAVAAAKRIGFPVALKIVSPEILHKTDVGGVRLHLADADAVAQGFAGIDATVRRLFPA
                     AAIHGIAVQEMVVGGKEVILGMTRDPQFGPLLMFGLGGIYVEVLKDVAFRVAPIGPDE
                     AEVMIREIRSFPLLGGVRGEKPSDLRAIVEALGRLSQLCMDFPEILELDVNPLLVKPE
                     GGGAVAIDARLALAA"
     gene            2657..>3684
                     /locus_tag="AC16G0_14505"
     CDS             2657..>3684
                     /locus_tag="AC16G0_14505"
                     /inference="COORDINATES: protein motif:HMM:NF024891.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransacetylase family protein"
                     /protein_id="HIR5100862.1"
                     /translation="MRTLFIGSTHGNSGKTMLGMGLAPVLQENGYTVGYLKPLGKQPV
                     TVKGKVVDADAQFLREVLNLAEPLEQICPVVLTQDLVARGLRGEAGDIWPKIRQAYEA
                     VSKGKDFLLIGGAGTLWDGGFLGISGPRLAKEFHAPVLLVDPYVNEVCVDCILMAKEL
                     LGDQLIGVILNRVPPQGLPEVEQMIVPFLGTQGIEVLGVLPLDRLLDAITVRQIVDVL
                     DGKVLCCEDSLDEFVERFSVGAMDVEHALGYFQKLPNKAVITGGSHADIQLAALETST
                     KCLVLTGDQMPNPIIVGRATELRVPLISVRYDTLTTVEKLEAVLGRIRIREAKKVERA
                     RELLRDHL"
BASE COUNT          648 a         1198 c         1227 g          611 t
ORIGIN      
        1 gcagatggtg gaggacgctc gctaccacgt ggcgggacgg cgccccgtct ccttctacgg
       61 ccgggtgggc ggcatgatca tggccccaga ggagctggtg gacaaggctc tcaagctcgt
      121 cgggaaacag ggctgaggca gagcgccgct tcacgcaggc cttccagagc agcagggtgg
      181 taccccgcct ctcctccacc gcgtcggcct ccgtggggga cgccgcggat ctgtgtgcag
      241 agctgctcgg gtccgagtct ccaaagtcag aaggacgcgc acggccgttc gggcacagcc
      301 ctccaggtgt cggtgggtgc accccggaag acgtggctgg gggaggctgc ttacagccga
      361 gatcccgacc ggtaagatga agccccctag acaggcagaa ctgtaggcgg cctgcgcgca
      421 atacgcgaca aggctccaat cttcgagcgc aagaggagac accatgctgg acggattctt
      481 caagcccaca gccgtgacgg tggtcggtgc ctcccgggaa gaagggaagg tcgggtacag
      541 tatcgttctc aacctcattc gcgggaagtt caccggaccc atttaccccg tgaatcccaa
      601 ggccagcgag atcctcggcc tcaagtgtta cccggacgtg accagcgtgc ctgcggacgc
      661 cgatctcgcc gttctggtca tccccccgaa gatctgcatc cccaccctgg agacctgtgc
      721 gggcagaggc atccgttcgg ccatcgtgat ctcggccggt ttcaaagagg tcggtggcga
      781 gggggccgag atcgagaagg ccctgcgcgc gcgcgtccgg gaactgggaa ttcgcgtggt
      841 cgggccgaac tgcctgggaa tcatctccac ccagagccgg atgaacgcca ccttcgccgc
      901 cggcatgcct ccggaagggg gaatctcatt cttctcccag tcgggggcgc tctgcacggc
      961 aatcctggac tgggccatcg gcaacaacgt gggtttcgcc aagttcatca gcctggggaa
     1021 caaggcggac atgtcggagg tggacttcat tcaggccctg gccgaggacc ccgagacccg
     1081 cgtcatcgtc ggctacatcg agggcgtgga ggacggtcgc cggttcatgg aggtggcccg
     1141 ggccgccagc cggaccaaac cgttcatcat cgtaaaatcg ggtggcaccg ccgctggggc
     1201 gcgggccgcc tcctcgcaca cggggacctt ggccggttcc gagaagtcct tcaacgcggc
     1261 cttccgccag tcgggggtca tccgggccga gagcatcgaa gaacttttca actacgcccg
     1321 ggccttcgcc gaccagccgc tcccccaggg accgagcctg tgcatcgtga ccaacgcggg
     1381 cggtcccggc atcattgccg cggatgcggt ggagcggtcc acggtaaaga tggccagctt
     1441 gtcccccgag acggtggagc gcttgcgggc gaccctgccc cctaccgccg ccctgtacaa
     1501 ccccgtggac gtgatcggcg acgccaagga agatcgctac aaggccgccc tggacgccgt
     1561 ggcggctgac gcgcacgtgg atggggtgct cgccctggcc actccccagg acatgacgga
     1621 gtacgacaag ttcgccgagg tggtcggaca ggcagccaag gcgtcgagca aacccttctt
     1681 caccgcgttc atgggtgagg cgagcctgcc caaggcccgc gagatcctgc ggagacacgg
     1741 cgtcccgcaa tacccctatc cggaacccgc ggtgcgcacc ttcgaggcca tgctgcgcta
     1801 tcggcagtgg caggagtaca cgccgacgcc acccacagag ttccacgtgg acaagggaca
     1861 ggtcgcgttg acgatcctga gtgcacggcg cacacggcgg acgcagctcg gcgagaccga
     1921 ggcccgcgag atcatcgccg cctacggctt ccgcctcccc cagaacgtcc tcgcccgaac
     1981 cgtggacgat gcggtggccg cggccaagcg gatcggcttc ccggtggccc tgaagatcgt
     2041 ctccccggag atccttcaca agaccgacgt cggaggggta cggctccacc tggccgatgc
     2101 cgacgccgtg gcccaggggt ttgccgggat cgatgccacc gtccggcggc tgtttcccgc
     2161 tgccgccatc cacggtatcg ccgtccagga gatggtcgtt ggcggcaagg aggtcatcct
     2221 gggcatgacc cgggacccgc agttcggccc gctcctcatg ttcggtctgg gcggtatata
     2281 cgtggaggtc ctgaaggacg tggccttccg agtcgcgccc atcggccccg acgaggcgga
     2341 ggtcatgatc cgcgagattc gttcctttcc gctgctcggc ggcgtccgag gggaaaaacc
     2401 ctctgacctt cgcgccatcg tggaggccct ggggcggctg tcccagctct gcatggactt
     2461 ccccgaaatc ctggagctgg acgtgaaccc gctgctggtc aagcccgagg gcggcggcgc
     2521 cgtggccatc gacgcccgtc tggccttggc ggcttgagcc aatgaccaat ttccaataac
     2581 caagtccccc cctctccctc ccttttccct acggtggggg agagggacag agtgaggggc
     2641 gcgagcggag gaagccatgc ggaccttgtt catcggctcg acccacggca attccggcaa
     2701 gacgatgctc ggcatgggcc tggccccggt cctccaggaa aacggctaca cggtcggcta
     2761 cctgaagccc ttgggcaagc agcccgtcac ggtcaaaggc aaggtggtag acgccgacgc
     2821 ccagttcctg cgggaggtcc ttaacctggc cgagcctctg gaacagattt gccccgtggt
     2881 cttgactcag gacctggtgg cgcggggtct gcggggtgag gcgggggata tctggccgaa
     2941 aatccggcag gcctacgagg ccgtgtcgaa gggcaaggac ttcctgctca tcgggggggc
     3001 gggcaccttg tgggacgggg gctttctggg aatctcgggc ccgcgtctgg ccaaggagtt
     3061 tcacgcgccc gtgctcctcg tggacccata tgtcaatgaa gtctgcgtgg attgcatcct
     3121 gatggccaag gagcttttgg gagatcagct catcggcgtg atcctgaatc gcgtaccccc
     3181 gcaagggctc cccgaggtgg aacagatgat cgtccccttc ctgggcacgc aaggcatcga
     3241 ggtcctgggc gtcctgcccc tggaccggct cttggacgcc atcaccgtgc gccagattgt
     3301 ggatgtcctg gacgggaagg tgctctgctg tgaggacagt ctggacgagt tcgtcgagcg
     3361 gttcagcgtg ggcgccatgg acgtcgagca tgccctgggc tacttccaga agctccccaa
     3421 caaggcggtc atcaccggcg gcagccacgc ggacatccag ctggccgccc tggagacatc
     3481 caccaagtgc ctggtgctga ccggcgacca aatgcccaac ccgatcatcg tcggcagggc
     3541 caccgagctc cgggtccccc tgatcagcgt gcgctacgac acgctgacca ccgtggagaa
     3601 actggaggcc gtcttgggtc ggatccgaat tcgtgaggcc aagaaggtcg agcgcgcacg
     3661 ggaattgctg cgggatcatc tgaa
//
LOCUS       DBNGZC010000842         3961 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_954510, whole genome shotgun sequence.
ACCESSION   DBNGZC010000842 DBNGZC010000000
VERSION     DBNGZC010000842.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_954510"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="AC16G0_14510"
     CDS             complement(<1..441)
                     /locus_tag="AC16G0_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR5100863.1"
                     /translation="MQPTGPENDAPRISPLTQEIISRLRERGLWVDVIVPENGPVDLA
                     DVRPRHDLYVLKSETPLALSLAGALTVAGATVVNTFRSSSLTRDKVAATAVLAAAGVP
                     VPPSWTTGQPGLLRPLLAEGTLWIKPQRGTQGVGIRRVATPEDLG"
     gene            complement(470..1261)
                     /locus_tag="AC16G0_14515"
     CDS             complement(470..1261)
                     /locus_tag="AC16G0_14515"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HIR5100864.1"
                     /translation="MATRLALDAGAALRRHQEGPLEVHRKANGEVVTPADLEADAIIR
                     TGLQAAFQQDAVFSEESADTPDRLESSRVWMVDPLDSTSNFTQRGNDYSVSIGLSLEG
                     RAVLGVVYNPGRDELFAGYQRVGVMLNGSPVRVTDVADLGHARLIVSRREWRRGLDKL
                     AGTLPILPVASIAYKLARVAAGLDDGMFSAIPRKQWDTCAGVALVEAAGGRATLLDGR
                     DLRFNLPPLKQPLGMVAGGPRLHPVLLEALRRLPLTDPLLRHPHD"
     gene            complement(1285..1713)
                     /locus_tag="AC16G0_14520"
     CDS             complement(1285..1713)
                     /locus_tag="AC16G0_14520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5100865.1"
                     /translation="MSTQTSAKTVLVIEDDRPILLVIKGHLEGAGYRVLGAGSGAEGL
                     ALARAGGVDLITLDVLMQPIDGWTVFQALRVDPSTRKIPIVFITILDEHPEGLVAEGY
                     VSKPFRARDLINVVGQALRTVPPHSGAHLQPGSPSARQRE"
     gene            complement(1716..2090)
                     /locus_tag="AC16G0_14525"
     CDS             complement(1716..2090)
                     /locus_tag="AC16G0_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5100866.1"
                     /translation="MADQKTVLVADDESILRRLVRATLRDRPLRILEAASGTETLALA
                     RRERPDLVLLDVGLPELDGYAVCRALKADPATASIKIVMLTARAQRHDLERGTAVGAD
                     AYITKPFSTQRLLADLDGFLRD"
     gene            complement(2083..3561)
                     /locus_tag="AC16G0_14530"
     CDS             complement(2083..3561)
                     /locus_tag="AC16G0_14530"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5100867.1"
                     /translation="MRHAHPEARPTWRLWGAPAGSGRELHQRMLGRWRRMSLLTRFTV
                     TGLFLTVTIALGLGMVIGRYLQERALTQAGEHLGEIVRLEIDQFLRPEDFAGVPSPER
                     THAIDVKIRDTLGEGRIVKINLWNPSGMVIYSTDSSLIGQRFPIDEELAEALRGEIGM
                     DISDLTKVENRIERARYSRLMEIYVPIRIGGGPVIGAYEVYRDVNGIMRMIATTQRLL
                     WIGLASGLGILYLSLFGVVRAASRRLHRQSEELGRLEARRMADQLTTEFVSIVSHELR
                     TPLTALVGFSELLLTQPVDSGEQREWIENMHAAAKRLTKLVEDLLDVSRIEEGRVELK
                     RQPIYVKAIVKLALADFRIHSSAHRFEERYRDPLPPLLADPDKLTQVLTNLLSNAVKY
                     SPRGGPITISVTAADGTVRLSVADQGLGLPPKELPRIFERFHRVQDEDRRQIPGTGLG
                     LYITQRLVELQDGRIWAESEGPGRGSTFHVELPAASGEKGHG"
     gene            complement(3558..>3961)
                     /locus_tag="AC16G0_14535"
     CDS             complement(3558..>3961)
                     /locus_tag="AC16G0_14535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100868.1"
                     /translation="WLRPAATVAAYLVPPAIAAASACFVIFSGARLFQPTLTRLTIGY
                     GIVLVGLAAGALAGRAASVSERLAVIRAYEQRLDDLDHDLRTPMTIIRGEVELVLSQE
                     NIPAAERTRSSAAILEQLGTLELRLRRRYQP"
BASE COUNT          701 a         1304 c         1279 g          677 t
ORIGIN      
        1 gcccagatcc tctggagtgg ccacgcggcg gatgccgact ccctgcgtgc cccgctgggg
       61 cttgatccag agtgtcccct cggccaagag gggccggagc aacccgggtt gacccgtggt
      121 ccacgacggg gggaccggga cgccggccgc cgccagaacg gcagtggccg caaccttatc
      181 ccgagtcagg ctggaggatc ggaacgtgtt gacgacagtc gcaccggcga cagtcaatgc
      241 cccggccagg ctgagggcca ggggggtctc cgatttcagg acgtacaggt catgcctggg
      301 ccggacatcg gccaggtcca ccggaccatt ctccggcacg atcacatcaa cccagaggcc
      361 gcgttctcgc aggcgactga tgatctcttg cgtgagcgga ctgatacggg gggcatcgtt
      421 ctccgggccc gttggctgca agaagaatgc gatcctcgca cggtgtgggc tagtcatggg
      481 ggtgccggag caaaggatcg gttaagggta ggcggcgcaa ggcttcaagc agaaccggat
      541 gcagcctcgg accgccggcg accatcccca ggggctgctt gagcgggggc aggttgaacc
      601 ggaggtcccg cccgtcgagc aaggtggctc gaccgcccgc ggcctccacc agcgccacgc
      661 ccgcgcaggt gtcccactgt tttcgcggaa tcgcggaaaa catcccgtca tccagccccg
      721 cggcgactcg cgccagcttg taggcgatgc ttgccacggg caggatcggg agcgtgccgg
      781 cgagcttgtc gaggccacgg cgccattccc tcctcgaaac gatgagccga gcgtgtccaa
      841 gatccgccac gtcggtgacc cgcaccgggg agccattcag catcacaccc acgcgttgat
      901 accccgcaaa aagctcgtct cgcccaggat tgtatacgac gccgagcacg gcccgacctt
      961 cgagtgaaag accgatggac acgctgtagt cgttgccccg ctgcgtgaag ttgctggtgc
     1021 tgtcgagggg atccaccatc cagacccgcg aagactcgag cctgtccggt gtatccgctg
     1081 attcctcgga gaacaccgca tcctgctgga atgccgcctg gagtccggtc cggatgatag
     1141 cgtcggcctc gagatcggcg ggggtgacga cctcgccgtt cgctttgcgg tggacctcga
     1201 gtggaccttc ctggtgacgg cggagcgcgg cgcctgcgtc caacgccaaa cgggttgcca
     1261 ccctgagttc gagatccaac atggtcattc gcgttgcctg gcagaaggac ttccaggttg
     1321 caggtgcgca ccggagtgcg gtggaaccgt cctcaacgcc tgccccacga cattgatgag
     1381 atcgcgggct cggaacggct tcgagacgta gccttcagcg accaagcctt cgggatgttc
     1441 atccaggatc gtgatgaaga cgatgggaat ctttcgggtt gacggatcga cccggagcgc
     1501 ctgaaatacc gtccaaccat caatgggctg catgagaacg tcgagcgtga tcaggtccac
     1561 gccgccggct cgggcgagcg ccagaccctc cgcgccgctg ccagccccga gcacccggta
     1621 gccggcacct tcgagatgcc ccttgatgac aagcaatatc ggccggtcgt cctcgatgac
     1681 cagcactgtc ttggctgacg tttgagttga catcgctagt ctcgcagaaa accgtcaagg
     1741 tcggccagga gccgctgtgt gctgaatggt ttcgtgatgt aggcgtctgc cccaacagca
     1801 gtgccccgtt cgaggtcgtg ccgctgcgcg cgcgccgtga gcatgacgat cttgatgctg
     1861 gccgtggccg gatccgcctt cagcgcccgg cacacggcat agccatccaa ttccggtaat
     1921 ccaacatcaa gcagcacgag gtcggggcgc tcacgccgcg ccagggccag cgtctcggtc
     1981 ccgctggcgg cctccaggat ccgcagcggc cggtcccgga gcgtcgcgcg gaccagccgc
     2041 cgaaggatgc tctcatcgtc ggcaaccagc acggtcttct gatcagccat gtcctttctc
     2101 cccgctggcg gccggcagct cgacgtggaa ggtgctgccg cgccctggcc cctcgctctc
     2161 ggcccagatg cggccgtcct ggagttcgac cagccgctgc gtgatgtaga gacccagacc
     2221 ggtcccggga atctgtcgcc gatcctcgtc ttgcacgcgg tggaaccgct cgaagatgcg
     2281 cggaagttcc ttgggcggca gccccagccc ctggtcggcg acgctcaggc ggacggtccc
     2341 gtcggcggcc gtcaccgaga tcgtgatggg gccgccccgc ggtgagtatt tgacggcgtt
     2401 ggatagaaga ttcgtgagga cctgggtcag cttgtccgga tctgcgagca gcggtggcag
     2461 ggggtcacgg taccgctcct cgaaacggtg ggccgatgag tggatcctga agtcggcgag
     2521 cgccagtttt acgatcgcct tcacgtaaat cggctgccgc ttcagctcga cccgtccctc
     2581 ttcgatccgt gacacgtcca ggagatcctc caccagcttg gtcagccgct tggcggcagc
     2641 gtgcatgttc tcgatccatt cgcgctgctc ccccgaatct accggctggg tgaggagcag
     2701 ctccgagaag ccgaccagcg ccgtcagcgg tgtccgcagc tcgtgagaga cgatgctgac
     2761 aaattcggtt gtaagctggt ccgccatccg acgggcttcg agcctgccca gctcctccga
     2821 ctggcgatgc agccgccttg aagccgcgcg caccaccccg aacagcgaga ggtagaggat
     2881 gcccaacccc gatgccagtc cgatccagag gagccgctgc gtggtggcga tcatgcgcat
     2941 gatcccgttc acatcacggt agacttcata cgcgccgatg acggggccgc cgccgattcg
     3001 aatggggacg tagatctcca tcagccggga atatcgagcg cgctcgatcc gattctccac
     3061 cttggtcagg tcactgatgt ccatcccgat ctcgcccctc agcgcttcgg cgagttcttc
     3121 atcgatggga aaacgctggc caatcaacga gctatccgtt gaatagatca ccatcccgct
     3181 ggggttccag aggttgatct tcacgatgcg tccctcgccg agcgtatcgc gaatcttcac
     3241 atcaatggca tgagtacgct caggcgaggg cacccccgcg aagtcctcag gacggagaaa
     3301 ctggtcgatc tcgagacgga cgatctcgcc caagtgctcc cccgcttgcg tcaaggccct
     3361 ctcttggaga taacggccga tgaccatgcc caggcccaac gcaatcgtga cggtcaggaa
     3421 aagcccggtc accgtgaagc gcgtcagcag gctcatgcgg cgccatcttc ccagcattcg
     3481 ctggtgaagc tcccggcccg acccggccgg cgccccccag agacgccagg ttggccgtgc
     3541 ctcgggatgt gcgtgcctca tggctggtac ctccggcgga ggcgcagttc cagcgtcccc
     3601 agttgctcga ggatcgcggc gctgctgcgc gtgcgctccg cggccgggat gttctcctgg
     3661 ctgagcacca gctcgacctc gccgcggatg atggtcatgg gcgtgcggag gtcgtggtct
     3721 aggtcgtcca gacgctgttc gtaggccctt atgaccgcga gacgctccga aacggaggcg
     3781 gcgcgcccag ccagcgcacc cgcagccagg ccgacgagca cgatgccata gccgatggtc
     3841 agccgggtca gcgtcggttg aaatagccta gccccagaga atatgacgaa gcaggccgag
     3901 gcagccgcga tggccggcgg cacgaggtag gcggcgacgg tagcggccgg ccgcaaccac
     3961 g
//
LOCUS       DBNGZC010000843         1804 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_267822, whole genome shotgun sequence.
ACCESSION   DBNGZC010000843 DBNGZC010000000
VERSION     DBNGZC010000843.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_267822"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="AC16G0_14540"
     CDS             <1..924
                     /locus_tag="AC16G0_14540"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5100869.1"
                     /translation="YDWNDFTQGARNDVTQPDKSISAIPTFVDVFTFFYRKDLFQEKG
                     IKVPRTMAELEDVAKKLHDPPRMYGFVSRGLKNANATPWAWVIYSLGGEYLTKDGKSA
                     INTPEWVKSLEWYSGMLRRYAPPGVVNFNWYECSSAFMQGQVAMYYDGVNFANQFEVP
                     EKSKIAGKVGYAVLPAGPVAHVAPTYTNAMAISAQSRNKEAAYLLIQWATMKKMCVRE
                     LLSGVGVGRVSPWSDPEVKAKPKMPADWYTAYLESLKIGRACLPEIVDVTQYRDIFGV
                     AIQKAIEGGNPAELVAQANKEFQEMLDKTEK"
     gene            1012..>1804
                     /locus_tag="AC16G0_14545"
     CDS             1012..>1804
                     /locus_tag="AC16G0_14545"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5100870.1"
                     /translation="MPNPVEQADVRRYAPGPDTAGGLGLWISRFTHRNARYIFPAPAF
                     LVVALIIVYPVIYTGWMSLQEWFASSLTVPKFIGVANYQKILTADPRFQHAFFLTIWF
                     TVVAVSAETALGLGMALLFNREFWGRGLVRTLSILPMMATPTAIALVFVMMYHPTLGV
                     MNYLVSLIGLPLLKWTYSSASALYALALVDVWEWTPLIMLISLAGLAALPKEPYESAR
                     IDGASAFQCFYLITLPLLRPTLVVAILFRAIDALKTFDIIFVMTQG"
BASE COUNT          324 a          592 c          552 g          336 t
ORIGIN      
        1 tatgactgga atgatttcac tcagggcgcc cgcaacgacg tcacccagcc cgacaagagc
       61 atcagcgcca ttcccacctt cgtcgacgtt ttcaccttct tttatcggaa agatctcttc
      121 caggagaagg gcatcaaggt tccccggacg atggccgagc tggaggacgt cgccaagaag
      181 ctccacgatc ctccgcgcat gtacgggttc gtctcccggg gcctgaaaaa cgccaacgcc
      241 acgccctggg cctgggtgat ctactccttg ggaggcgagt acctgaccaa ggacgggaag
      301 tccgcgatca acacacccga gtgggtgaag agcctcgagt ggtactctgg gatgctccgc
      361 cgctacgccc cgcccggtgt ggtgaacttc aactggtacg agtgtagcag cgcgttcatg
      421 caaggccagg tggcgatgta ctacgacggc gtgaacttcg ccaaccagtt cgaggtcccc
      481 gagaagtcca agatcgcggg gaaggtgggg tatgcggtgc tgcccgccgg gccggtggcc
      541 cacgtcgccc cgacctacac caacgccatg gccatcagtg cccagagccg gaacaaggag
      601 gcggcctacc tcctcatcca gtgggccacc atgaagaaga tgtgtgtgcg tgaattgctg
      661 agcggtgtcg gcgtgggccg ggtttcaccc tggagcgacc ccgaggtgaa ggccaagccc
      721 aagatgcccg ccgattggta caccgcctac ctggaaagcc tgaagatcgg acgcgcctgt
      781 ctgccggaga tcgtggacgt cacgcagtac cgggacatct ttggcgtcgc catccagaag
      841 gccatcgagg ggggtaatcc cgcggagctg gtcgcccagg cgaacaagga gttccaggag
      901 atgctagaca agaccgagaa gtagggccga ccgtcaacct gagcctggtc ctccggctcc
      961 cgggcatccc aagggtggga gggccaggct gtttttcagg agaggggcaa gatgccgaac
     1021 ccggtcgagc aagccgacgt ccgacggtac gcccccggtc ccgacacggc ggggggcctc
     1081 ggcctctgga tctcccgctt cacccaccgg aatgcccggt acatcttccc ggcgcctgcc
     1141 ttcctggtgg ttgccctgat catcgtctat cccgtgatct ataccggctg gatgagcctg
     1201 caggaatggt tcgcgtccag cctgaccgtg ccgaagttca tcggggtggc caattaccag
     1261 aagattctca cggccgaccc tcgcttccag cacgccttct tcctgacgat ctggttcacg
     1321 gtagtcgccg tctcggccga gacggcgctc ggcctgggaa tggcgctcct cttcaaccgg
     1381 gagttctggg ggcgcggcct ggtgcgaacg ctctccatcc tgcccatgat ggccaccccg
     1441 accgccatcg ccctggtctt cgtgatgatg taccatccca ccctgggggt gatgaactac
     1501 ctggtcagcc tgatcgggct tcccctcctc aagtggacct atagcagcgc ctccgccctc
     1561 tacgccctgg ccctggtgga tgtgtgggag tggacgccgc tgatcatgct catctccctg
     1621 gcggggctgg cggcgctccc caaggagccc tacgagtccg cccggatcga cggggcctcc
     1681 gcattccagt gtttctatct gatcacgctg ccgctgcttc gaccgacgct ggtcgtcgcc
     1741 atcctgttcc gggccatcga tgccctgaag accttcgaca tcatcttcgt catgacccag
     1801 ggcg
//
LOCUS       DBNGZC010000844         5565 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1733761, whole genome shotgun sequence.
ACCESSION   DBNGZC010000844 DBNGZC010000000
VERSION     DBNGZC010000844.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1733761"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="AC16G0_14550"
     CDS             complement(<1..303)
                     /locus_tag="AC16G0_14550"
                     /inference="COORDINATES: protein motif:HMM:NF042468.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase PUA domain-containing
                     protein"
                     /protein_id="HIR5100871.1"
                     /translation="MSDSRIHHRRFFVPAACIRDGWVEFEPAQAHQLARVLRLQRGDQ
                     VSVFDGTGREVIAALERTSPRLATARILREVPAPPANALAITLAQVVPRGAAMDLIL"
     gene            complement(397..1506)
                     /gene="dnaJ"
                     /locus_tag="AC16G0_14555"
     CDS             complement(397..1506)
                     /gene="dnaJ"
                     /locus_tag="AC16G0_14555"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR5100872.1"
                     /translation="MSHKRDYYEVLGVSRDAGADEIKKAFRKLAQQHHPDKNAGNESA
                     EERFKEIGEAYEVLGNPEKRQAYDRFGVAGPGRGFEGFGGFDPGFGSIFDDIFEGFFG
                     GRGGRSSRGAHRGADLRYNLEIRFEEAALGVEKQIVVPRLETCPACQGSGAKAGSQPI
                     VCKACRGSGQVRFSQGFLTVSQTCGQCRGEGRVIEHPCQTCRGLGQAKAERTLTVKIP
                     AGVETGTRLKLSGEGEAGVRGGPPGDLYVVLMVQEHPIFTRQGDDLYCEIPIKFTQAA
                     LGTQIEIPAISGRTTLKIPPGAQTGTEIRIKGRGLPNVRGYGRGDLVARIFVEVPTHL
                     TAKQRDLLEQYARLENGAGSPLVEGFWEKVKALFD"
     gene            complement(1503..2027)
                     /gene="grpE"
                     /locus_tag="AC16G0_14560"
     CDS             complement(1503..2027)
                     /gene="grpE"
                     /locus_tag="AC16G0_14560"
                     /inference="COORDINATES: protein motif:HMM:NF013212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR5100873.1"
                     /translation="METSPEALREAFTAKSQEVERLQERLLRLHAEFENYRKRMAREK
                     AEFLKFANEGLILQILPVLDNLERAITSARAEVGSTPLIEGIEMIARLFRTTLEKAGV
                     KPMEALGQPFDPGFHQAVAQADSSSAEANLVIEEIQKGYLLEGRVLRPAMVKVSRGAP
                     SATEDGGAAEGNRA"
     gene            complement(2164..3195)
                     /gene="hrcA"
                     /locus_tag="AC16G0_14565"
     CDS             complement(2164..3195)
                     /gene="hrcA"
                     /locus_tag="AC16G0_14565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR5100874.1"
                     /translation="MLNARHQGVLRAIVLDYIRTAEPVGSRTISRKYGFPLSPATIRT
                     IMADLEELGYLAQPHTSAGRIPTDQGYRFYVDALMDRPALSRAEVERIEHQVAPEAGE
                     VDELLREAGKLLSALSPYVALVLSPRVDESHFRRVEFVSLTHDRVLVILMSDTGVVYH
                     KVVVVDEPMGQEDLDRIGRYLTDLLHGKSLQKVRDLLLAQMAEEKAQYDRLLAQALQL
                     GAKALEGAVEADVYVAGAAHIADQPEFADIGKMKALYSAFEEKSKLVKILNECLSGEG
                     CRIFIGREIPVEDIQELSLIAAPYRRGDRALGVLGMLGPTRMDYGRALALVETTATLL
                     TRVLTEGAA"
     gene            complement(3390..4070)
                     /gene="lepB"
                     /locus_tag="AC16G0_14570"
     CDS             complement(3390..4070)
                     /gene="lepB"
                     /locus_tag="AC16G0_14570"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR5100875.1"
                     /translation="MTVQRTVEEEKHTEPRKTGRALRPKSAFREYAEALIIAVLLALV
                     IRTFVVQAFKIPSGSMLPTLQIGDHILVNKFLFYFTPIKRSDIIVFKFPHDESRDFIK
                     RVIGLPGETLEIRGRQVLINGTPLSEPYAVFADGPLGRGLEREHVGPLVIPPDHLFMM
                     GDNRDRSMDSRVWGFLDVHKVKGKAFLVYFSIRSEDIPYSSILPGVVYVVAHPSLIRW
                     DRFGRLIY"
     gene            complement(4205..>5565)
                     /gene="lepA"
                     /locus_tag="AC16G0_14575"
     CDS             complement(4205..>5565)
                     /gene="lepA"
                     /locus_tag="AC16G0_14575"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR5100876.1"
                     /translation="EEILALDGSDAIPVSAKHGWGTAEVLEAIVAKIPHPTGSPSAPL
                     KALIFDSWFDSYQGVIVYVRLYDGSVAPGMRIQLMSSGATFEIGEVGIFTPQMRAADR
                     LCAGEVGYIIAGIRDVRQSRVGDTITGAEHPTARPFPGFREVRPMVFAGLYPVESQDY
                     LNLKGALEKLQLNDASFSFAPETSLALGFGFRCGFLGLLHLEIVQERLEREFGLTLLT
                     TAPTVVYRVIRTDGSVMEVENPSKLPPPQEIAAIEEPYISASIMAPTEYVGAILALCQ
                     EKRGVHKGIEYPQDSRVLIAYELPLNEIVLDFYDRLKSVSRGYASLDYDFLDYRESPL
                     IKLDILVNGEPVDALSFIIHRDQAATRGRLLAERMRELIPRQLFEVVIQAAIGGRVIA
                     RERIAPLRKAVTAKCYGGDITRKRKLLEKQAAGKRRMKQVGRVEIPQEAFMALLTVGD
                     DN"
BASE COUNT         1009 a         1842 c         1682 g         1032 t
ORIGIN      
        1 caggatgagg tccatggccg cccctcgggg aaccacctga gccagcgtga tggcaagagc
       61 gttggctggc ggtgccggca cctcgcggag aatccgagca gtcgccaggc ggggggacgt
      121 gcgctccaag gcggcgatca cctctcgacc ggtcccgtcg aagaccgaga cctgatcccc
      181 ccgttggagg cgaaggacgc gcgccaactg gtgcgcttga gccggctcga attctaccca
      241 gccatcgcgg atgcacgcgg ccggaacgaa gaacctgcga tggtggatcc tcgagtcgct
      301 catggggtga ggagggccag tgcgcaatcg acaacggaca gctgacaacc cgcggagaaa
      361 gcagtcggcg ggtccaaccg aggccggcga ctgacctcag tcgaacaagg ccttcacctt
      421 ctcccagaat ccctcgacca atggactgcc ggcgccgttc tccaggcggg catactgctc
      481 caggaggtct cgctgtttgg ccgtgaggtg ggtgggcacc tccacaaaga tccgggccac
      541 cagatcgccc cgaccgtacc ctcggacgtt cggcaacccc cggcccttga tccggatctc
      601 cgtgccggtc tgcgcccccg gagggatctt gagggtggtt cgacctgaaa tggcgggaat
      661 ctcgatctgg gtccccagcg ccgcctgcgt gaacttgatg ggtatctcac agtagagatc
      721 gtcgccctgg cgggtgaaga tcggatgctc ctgcaccatc aggacgacgt acaagtcccc
      781 aggcggtcca ccacggaccc ccgcttctcc ctcgccgctc agcttgaggc gcgtccccgt
      841 ctcgacgccg gcgggaatct tcaccgtcag ggtccgttcg gccttggcct gtcccaaccc
      901 ccggcaggtc tggcagggat gctcgatcac ccgcccctcg ccccggcact ggccgcaggt
      961 ttggctgacg gtcaggaagc cttgggagaa ccggacctgc cccgagcccc gacaggcctt
     1021 gcagacgatg ggctgcgagc ctgccttggc cccgctgccc tggcaggccg gacaggtttc
     1081 gagccgcggg accacgatct gtttttcgac cccgagggcc gcttcctcga aacggatttc
     1141 caggttatag cgaaggtccg ccccccggtg cgcgccccgg ctggagcgcc cgcctcgccc
     1201 gccgaagaag ccctcgaaga tatcgtcgaa gatgctgccg aaccccggat cgaacccccc
     1261 gaatccctcg aatccgcgtc cgggtccggc cacgccgaag cggtcatagg cttgccgctt
     1321 ctcgggattg ccgaggacct cgtaggcctc gccgatttcc ttgaagcgct cctcggccga
     1381 ctcattgccg gcgttcttgt ccgggtggtg ctgctgcgcg agcttgcgga acgccttctt
     1441 gatctcatcg gcaccggcgt cccgagaaac ccccaagacc tcgtagtaat cgcgcttgtg
     1501 actcacgccc gattgccctc agcagccccg ccgtcctccg tcgcggatgg ggcgccacgc
     1561 gagaccttga ccatggccgg acggagcacg cgaccctcca gcaggtatcc cttctgaatc
     1621 tcctcgatca cgagatttgc ctctgccgac gaggaatcag cctgggccac cgcctggtgg
     1681 aagccggggt cgaagggctg tcccagggcc tccatgggtt tcacgccggc cttctccaac
     1741 gtggtgcgga acaggcgggc gatcatctcg atgccctcga tgagcggcgt ggaaccgacc
     1801 tcggcgcggg ccgaggtgat cgcccgctcc aggttgtcca ggacgggcag gatctgaagg
     1861 atcaggccct cattggcgaa cttcagaaac tccgccttct cgcgggccat gcgtttcctg
     1921 tagttctcga actcggcatg caggcgcagc aagcgctctt gcagccgttc gacctcttgg
     1981 gatttcgccg tgaaggcttc gcgcagggcc tccggagagg tctccaccgc gggcaactct
     2041 ggggacgcgg ccgaagtcgg gggcgtctca acgggagtcg gggataattc gttttgttgt
     2101 tccatggctc cagacacacc ggacgggaga gatatttcct cccgcccggc aaccgttagg
     2161 gggttaggcc gcgccctctg tcaggacacg ggtgagcagc gtggccgtgg tctccaccag
     2221 ggccagggcc cgtccataat ccatgcgtgt tggcccgagc atcccgagga cgcccaaggc
     2281 tcgatcccct ctgcgatatg gcgcggcgat caggctcaat tcctggatgt cctccacagg
     2341 gatctcacga ccgatgaaga tcctgcagcc ttcgccggag agacactcgt tcaggatctt
     2401 caccagcttg gatttttcct cgaaggcgga atacagggcc ttcattttgc cgatgtcggc
     2461 aaactcgggc tggtcggcga tgtgggcggc accggcaacg tacacatccg cctccaccgc
     2521 accctccagc gcctttgccc cgagttggag cgcttgcgcc agcagccgat cgtactgcgc
     2581 cttctcttcc gccatttggg ccagcaggag gtcccgcacc ttttgcaggc tcttgccgtg
     2641 caacagatcc gtcagatacc ggccgattcg gtcgaggtcc tcctggccca ttggctcgtc
     2701 cacgaccacc accttatggt agaccactcc cgtatccgac atcaggatga ccaagacccg
     2761 atcgtgcgtg agagacacaa attccacccg ccggaaatgg ctctcgtcta ctcgcggtga
     2821 gagaaccaag gccacgtacg gagagagcgc cgacaggagc tttccggcct cgcgcagcaa
     2881 ctcgtccacc tcgcccgcct ctggcgccac ctggtgttcg atccgctcga cctcggcccg
     2941 gctcaatgcg ggacggtcca tcagcgcatc gacataaaat ctgtagccct ggtccgtcgg
     3001 aatcctgccg gcggaggtgt gcggctgagc caagtagccc aactcctcca ggtccgccat
     3061 gatggtgcga atagtcgcag ggctcagcgg gaatccgtac ttgcgggaaa tggtgcggga
     3121 acccaccggt tcagccgtac gaatgtaatc caggacgatg gctcggagca ccccctggtg
     3181 tcgagcgttg agcatgagga cacctcgaac actcagcact ccgcgacgaa gagtgctaaa
     3241 ttttcattca ctatagagaa gcccccagga gttgtcaaga gtcgagcccc ctgcggctca
     3301 atctctggaa ccccaaaggc ggcgaattca tgaacgtttg tggctagttg gcggggagac
     3361 agtcggtttc tgtccgttct tctcatccct caatagatga gcctcccgaa ccgatcccat
     3421 cggatgaggg acgggtgcgc aaccacgtac accacgccag gcagaatgga tgaataggga
     3481 atgtcctcac tccggatgga gaaatacacc agaaaggcct ttccttttac cttgtgcaca
     3541 tccaggaatc cccagactcg actgtccatg ctccggtccc ggttatcccc catcatgaag
     3601 aggtgatcgg gcgggatgac gagagggccg acgtgctccc gctccaatcc gcggcccaac
     3661 ggcccatccg caaacaccgc atagggttcg ctgagggggg ttccattgat gagcacctgg
     3721 cgtccgcgga tttccagggt ctctcccgga agcccgatga ctcgcttgat gaagtcccgc
     3781 gactcgtcat gagggaactt gaagacgatg atatcgctgc gtttgatcgg cgtgaaatag
     3841 aagagaaact tgttgaccag gatgtggtcc ccgatctgga gcgtgggcag catggatccc
     3901 gaggggatct tgaaggcttg caccacgaag gtccggatca ccagggccaa tagcaccgcg
     3961 atgatcaggg cctcggcgta ttcccggaaa gccgatttgg gacgcagagc ccgtccggtc
     4021 tttcttggct ctgtgtgttt ctcttcctcg actgtcctct gcacggtcag cttctcctca
     4081 aggtgccaac gcaagaccga caactgagaa tgccaattga aaacggacaa ccgacaactg
     4141 acaaccgacc aagttcttca cgtggtcttc cgttggccgg ttgtcggttg tcagtttctc
     4201 attttcaatt gtcgtccccc accgtcagca aggccatgaa cgcctcctgg gggatctcca
     4261 cgcggccgac ctgcttcatg cggcgcttcc cggccgcctg cttctccagg agcttccgct
     4321 tccgcgtaat gtcacctcca tagcacttgg ccgtcacggc tttcctgagg ggagcgatcc
     4381 gctctcgggc gatcacccgg ccgccgatgg ccgcttggat caccacctcg aagagctggc
     4441 gtgggatgag ttcccgcatc cgctccgcca agagtcgccc gcgcgttgcg gcctggtccc
     4501 ggtgaatgat gaaggacagg gcatccaccg gctcgccatt caccaggatg tccagcttga
     4561 tcagcggact ctcccggtag tccaggaagt cgtagtccag cgaggcgtac ccccggctca
     4621 ccgacttgag tcggtcgtag aagtccagga cgatttcgtt cagcggcaat tcatacgcga
     4681 tgaggacccg cgagtcctgc gggtactcga tacccttgtg gaccccccgt ttctcctggc
     4741 acagggccag gatcgccccc acatattccg tgggcgccat gatggaggca ctgatgtagg
     4801 gctcttcgat ggcggcgatc tcctggggcg gcggcaactt gctgggattc tccacctcca
     4861 tcaccgatcc atccgtccgg atcacccgat agaccacggt gggcgccgtc gtgaggaggg
     4921 tgaggccgaa ctcccgctcc agccgctcct ggacgatttc caggtgcagg agtcccagga
     4981 acccgcagcg aaacccgaag cccagcgcca gcgacgtctc gggggcgaag gagaaggagg
     5041 catcgttgag ctgcagcttc tccagcgcgc ccttgaggtt gagatagtcc tggctctcca
     5101 ccggatacag gccggcaaag accataggcc gaacctcgcg gaagccggga aatggccgtg
     5161 ccgtggggtg ctccgccccg gtgatcgtgt ccccgacgcg gctctggcgc acatcccgga
     5221 tgcccgcgat gatgtacccg acctctcccg cgcagaggcg atcggccgct cgcatttggg
     5281 gcgtgaagat gccgacctcg ccgatctcga aggtcgcccc gctggacatg agttgaattc
     5341 gcatcccggg tgcgacgctc ccgtcataca gcctgacata cacgatcacc ccctggtagc
     5401 tgtcgaacca ggagtcgaag atcagcgcct tgagcggcgc actgggcgat cccgtggggt
     5461 gcgggatctt ggcaacgatg gcctccagca cctccgccgt cccccagccg tgcttggcgc
     5521 tgaccgggat ggcgtcgctc ccgtccaggg ccaggatctc ctcca
//
LOCUS       DBNGZC010000845         4274 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1834830, whole genome shotgun sequence.
ACCESSION   DBNGZC010000845 DBNGZC010000000
VERSION     DBNGZC010000845.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1834830"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..2269)
                     /locus_tag="AC16G0_14580"
     CDS             complement(170..2269)
                     /locus_tag="AC16G0_14580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR5100877.1"
                     /translation="MALENPVLGNRPLRYSVCPHDCPSTCALEVEVQDGLRIGRIRGA
                     KANSYTAGVICEKVSRYAERVHHPDRLTRPLLRVGSKGSGAFREIGWDEALDRAAERF
                     LEAAARFGSEAVWPYYYAGTMGLVMRDGINRLRHVMRYSRQRKTICTGSAEVGWIVGT
                     GRFAGPDPREIPKADLIVVWGGNPAATQISLMTHIARARKERGAKLVVVDPYRSDTAA
                     LADLHLCLRPGTDAALACALMHCAFRDGHADRAYLQTYTDYSADLEAHLAACGPDWAA
                     GITGLTVEEIEAFARLYNTTARSYIRVGYGFTRSRNGSAAMHAVTCLPAVTGAWTHEG
                     GGAFWSNRSIYHWDKTMIEGLDALDPATRELDMCRIGAALTGDAEALRGGPPVAAMLI
                     QNTNPAVVAPDGNRVRRGLAREDLFVVVHEQFMTDTARYADVVLPATMFLEHDDLYQA
                     GGHSHIQIGPKLVEPPGECRSNHAVLQGLARRLGAKHPGFELTAMELIDWTLHASGWP
                     DAKTVMEERWVDAALPYREAHFLDGFGTPDRKFHFAPDWSRVGPDWKRMPRLPDQLDV
                     IEAATADKPFRLVTSPAKSFLNTSFTETPGSRKRERRPTALIHPADGDRLGLADDDSV
                     TIGNDRGEVTAHVRRFDGLQPGTVVVEGIWPNRDFEAGVGVNTLVGDDPIPPFGGGAF
                     HDTAVWLRPTARPTISS"
     gene            complement(2382..>4274)
                     /locus_tag="AC16G0_14585"
     CDS             complement(2382..>4274)
                     /locus_tag="AC16G0_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5100878.1"
                     /translation="EERLKLFIQNAPAAIAMFDRDMRYLAVSRRWLTDYGLGDRNIVG
                     HSHYEVFPDIPDHWKTAHQRGLAGEVVQATEDRFQRMDGTVHWLRREVRPWHKADATV
                     GGIILFTEDITERRRAEEEIRSLAKFPSENPYPVLRTTVDGTILYANLASLPLLETWK
                     TGRERILPQPWRESVRHVYAAGVPRELEAVSGERVFSIILVPILDAGYVNIYGRDITD
                     RKRAEAALRKAHDELELRVQERTTELSTAIQTLEDQSEQLRALASELTLAEQRERRRL
                     ATVLHDEHQQLLVGVKLRISPLERAQDPTVREACREITSLLEEAIEHARSLTRELSPP
                     ILQAGGLIPAMNWLARWMGEKHHLTVELRTDETAVPATEDLIILLFQAVRELLFNVVK
                     HAKVPTARLELRGEDGQVQLIVFDAGVGFDPASLRIQDATGFGLFSIRHRLELLGGRM
                     EIDSFPGQGSRITLWSPLRGATATVQPLSGQPGGPESAGIAEGASPARSRTGKIRVLV
                     VDDHRVVRQGLANLLKAEPDIEIVGEAADGETAVILTRQLLPDVVTMDVSLPGMNGIE
                     ATRVIHTECPAVQVIGLSMFEEAEQAKAMRDAGAADYLTKSEASESLVAAIRACAETK
                     RQGQGH"
BASE COUNT          675 a         1409 c         1422 g          768 t
ORIGIN      
        1 agcggctggt ttcggatttc cctcgattcg tgcggcagat tactgggtgg gccaatgcga
       61 atcccatggt gtagaagccg aagttggaat cttcggatgg ggccagatat ccgcgggcgc
      121 tcccccgctc cggaaacttg gggagcatgg accagcccgg gaccgggtgt caagaagaaa
      181 tagtggggcg ggcggtgggt ctcagccaca ccgcagtgtc gtgaaaggcg ccgccaccga
      241 agggcgggat cggatcgtcg ccgacgagcg tattgacacc cacgccggcc tcgaagtccc
      301 gattcggcca gatgccttcg accacgactg tccccggctg aaggccatcg aaccgacgaa
      361 cgtgcgcagt cacctcgccc cggtcattgc cgatggtgac agagtcgtcg tcggcgaggc
      421 cgagtcggtc gccgtcagcc gggtggatga gcgcggtcgg tcgccgctcg cgcttccgcg
      481 aaccgggcgt ctcggtgaat gaagtgttga ggaagctctt ggcgggggaa gtcaccaggc
      541 gaaacggctt gtcggcggtc gcggcctcga tgacgtccaa ctggtcggga agtcgcggca
      601 tccgtttcca gtcggggccg accctcgacc agtccggagc aaaatgaaac ttcctgtcgg
      661 gcgtgccaaa cccgtccagg aagtgagcct cacggtacgg cagggccgcg tccacccaac
      721 gctcttccat cacggtcttg gcgtccggcc aaccggaggc gtggagcgtc cagtcaatga
      781 gttccatggc ggtcagctcg aaacccggat gcttggctcc cagccggcgt gcgagtcctt
      841 gcaggaccgc gtgattcgac cggcattcac ccggtggctc gaccaacttg ggccctatct
      901 ggatatggct gtgaccgccc gcctggtaga ggtcgtcgtg ctccaggaac atggttgccg
      961 ggaggactac atcggcatag cgggccgtgt cggtcatgaa ttgctcgtgc acgacgacga
     1021 acagatcctc gcgcgcgagg ccacggcgca cgcggttccc gtccggtgcc accacggccg
     1081 gattggtgtt ctggatgagc atggcggcga cgggtggtcc gccccgcagc gcctcggcgt
     1141 cccccgtgag cgcagcgccg atgcggcaca tgtccagctc gcgcgtggcg ggatccaggg
     1201 catccagccc ttcaatcatg gtcttgtccc agtggtagat cgagcgattg ctccagaagg
     1261 cgccgccccc ttcgtgggtc caggcgccgg tgacggcggg gaggcaggtg acggcatgca
     1321 tggcggccga gccgttgcgc gagcgggtga agccgtaccc gacccggatg taagaccgcg
     1381 cggtcgtgtt gtacagcctg gcgaaggctt caatctcctc gaccgtgagg cccgtgatgc
     1441 cggcggccca atccggcccg caggcggcga gatgggcctc gagatcggcc gagtaatcgg
     1501 tgtaggtctg gagataggcg cgatcggcgt ggccgtcccg gaaggcgcag tgcatcagtg
     1561 cgcaggccag ggcagcatcc gtgccggggc ggaggcacag gtgcagatcg gccagcgctg
     1621 ccgtgtcgct ccgataaggg tccaccacga ccagcttcgc cccgcgttcc ttccgggccc
     1681 gggcgatgtg cgtcatcagg ctgatctgcg ttgcggccgg gttgccgccc cagacgacga
     1741 tcagatccgc tttcggtatc tcccgcggat cggggccggc gaaccggccg gtcccgacga
     1801 tccagccgac ttcggcggag cccgtgcaga tcgtcttccg ctggcgcgag tatcgcatga
     1861 cgtggcggag gcggttgatg ccgtcgcgca tcaccaggcc catggtcccg gcgtaatagt
     1921 agggccacac ggcctcggac ccgaagcggg ctgcggcttc caggaagcgt tccgccgccc
     1981 ggtcgagggc ctcgtcccag ccgatctcgc ggaacgcccc cgaccccttt gacccgacac
     2041 ggagcagcgg tcgggtcagc cggtcggggt ggtggacgcg ctcggcatag cggctgacct
     2101 tttcacagat cacaccggcg gtgtagctgt tcgccttggc ccctcgtatt cggccgatac
     2161 gcaggccgtc ctggacctcg acctcgaggg cgcaggttga cgggcaatcg tgtgggcaca
     2221 cggaatagcg taggggacgg tttcccagca ccggattttc caaggccatg ggggctcctt
     2281 cctacttctg ggtcgggcat cgcggctcgc cacccgatgg cgatcgcggc acgtctgtgg
     2341 cacgctgagg cgagcggggt gccgcacgtc agtggcgatt gtcaatgacc ctggccctgc
     2401 ctcttggtct cagcgcaggc ccggatggct gcgaccaggg attcggacgc ttcgctcttt
     2461 gtcagatagt ccgccgcccc ggcgtcccgc atcgcctttg cctgctcggc ctcctcgaac
     2521 atggagaggc cgatcacctg tacggcgggg cactcggtgt ggatgacccg tgtggcctcg
     2581 atgccgttca tccccggcag gctcacgtcc atggtcacca cgtcgggcaa gagctggcgg
     2641 gtcaggatca cggccgtctc cccatccgcc gcctcgccca cgatctcgat gtccggctcg
     2701 gccttcagca agttggccag gccctgccga acgaccctat gatcatccac caccaggacg
     2761 cggatcttcc ctgtccggct gcgagctggc gaggcgcctt cggcaatgcc ggcgctctcg
     2821 ggcccgcccg gctgcccgga gagcggctgg acggttgccg tggcaccccg gagcggggac
     2881 cagagcgtga tccggctgcc ctggccaggg aaactgtcga tctccatccg gcctcccagc
     2941 agctccagcc gatggcgaat gctgaagagg ccaaaaccgg tggcgtcctg tatgcggagg
     3001 gaggctggat cgaagcccac gcccgcgtcg aagacgatga gctgtacctg gccgtcctcc
     3061 cctctgagct cgagccgggc ggtcggcacc ttggcatgct tcacgacgtt gaacagcagc
     3121 tcccgcacgg cctggaagag caggatgatc aggtcttcag tcgcgggaac ggcggtctcg
     3181 tcggtccgca gctcgaccgt gagatggtgc ttctccccca tccagcgtgc cagccagttc
     3241 atggcaggga tcaggccgcc ggcctgcaag atcggcgggc tgagttcccg tgtgagggac
     3301 cgggcgtgct cgatagcctc ttccagaagg gaggtgatct ccctgcacgc ctcccgcacc
     3361 gtcggatcct gtgccctctc caaggggctt atcctgagtt tcacgcccac caggagttgc
     3421 tggtgttcat catgcagcac cgtcgccagc cgccggcgtt cccgctgctc ggccagcgtc
     3481 agctccgatg ccagcgcgcg gagctgctcc gattgatctt cgagtgtttg aatcgccgtg
     3541 gacaattcgg tcgtccgttc ctggacccgc aattccaatt cgtcatgcgc tttccgcaga
     3601 gccgcctcgg cccgctttcg gtcggtgatg tcccgtccgt aaatgttcac gtagcctgcg
     3661 tccaggatcg gcaccaggat gatcgagaag acgcgctccc cgctgacggc ctccagctcc
     3721 cgcggcaccc ctgccgcata cacatgtcgc acgctctccc gccatggctg gggaagtatc
     3781 cgctccctcc ccgttttcca cgtctcgagc aaggggagac ttgcgaggtt agcatacagg
     3841 atcgttccgt cgacagttgt ccggaggacc gggtatggat tctcacttgg gaacttcgcg
     3901 aggctacgga tctcctcttc agcgcgcctg cgctcggtga tgtcctcggt gaatagaatg
     3961 atgccgccga ccgttgcatc cgccttgtgc cagggccgta cctcccgccg gagccaatgc
     4021 accgtcccgt ccatccgctg gaagcggtct tccgtggcct gcacgacctc gccggccagg
     4081 ccgcgctggt gcgcagtctt ccagtggtcg gggatatcgg gaaagacctc gtagtgggag
     4141 tggccgacga tgttccgatc acccaggccg tagtctgtca accagcggcg gctcacggca
     4201 agatagcgca tgtcccggtc gaacatggcg atggctgccg gcgcattctg gatgaagagc
     4261 ttgagccgct cctc
//
LOCUS       DBNGZC010000846         1530 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_74022, whole genome shotgun sequence.
ACCESSION   DBNGZC010000846 DBNGZC010000000
VERSION     DBNGZC010000846.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_74022"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..892)
                     /gene="lptB"
                     /locus_tag="AC16G0_14590"
     CDS             complement(167..892)
                     /gene="lptB"
                     /locus_tag="AC16G0_14590"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HIR5100879.1"
                     /translation="MERSLKTEKLVKSFKQRVVVSDLSLSLDAGEVVGLLGPNGAGKT
                     TAFYMVLGLARPDSGRVFLNGDDVTFLPVHQRARRGMGFLPQEPSIFRKLTVEENILA
                     ILEAVGLPAPERAGRTEALLRELNIAHLANSRAYTLSGGERRRVEITRALATDPSFML
                     LDEPFAGIDPIAVYDIQGIIGQLKDRGIGVLITDHNVRETLQIVDRAYIIHEGQILLS
                     GTAAELAADERAREIYLGQRFTL"
     gene            complement(920..1429)
                     /gene="lptA"
                     /locus_tag="AC16G0_14595"
     CDS             complement(920..1429)
                     /gene="lptA"
                     /locus_tag="AC16G0_14595"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HIR5100880.1"
                     /translation="MTPVRWLLLFNVLLLVMPGTLRAAEERTPLSISADSLEVNRKDR
                     VAIYRGSVAASDKGRGLSIQADQIEFFFDEKMEDVERALATGNVRIAYGERRGVAQRA
                     EYYPGENRAVLLGHPRVWQDSDVVTGCKITLLMREDRSQVDGCEGERVNAVLYPKRGA
                     GPERAPTRR"
BASE COUNT          247 a          532 c          477 g          274 t
ORIGIN      
        1 atcgcctgct gcagcatcgg cgtcatgatc atccgctgcg tctgcagcat ccggagcttg
       61 gtgtcaaaag ccataggacg ctggcacagg ggaaagcgga agaggacgcg ggcctcgggt
      121 atcggcccca gctcctcgga tctctgctcc ccgcctcgtg cctccgttac agggtgaagc
      181 gctgccccag atagatctcg cgagcccgtt cgtccgccgc cagttcggcg gcggtgccgg
      241 acaagaggat ctgtccttcg tgaatgatat aggctcggtc cacaatctgc aaggtctctc
      301 gtacgttgtg atccgttatc aggaccccga tgccgcgatc cttaagctgg ccaatgatcc
      361 cctggatgtc gtacacggcg atggggtcga tcccggcgaa gggttcgtcc aggagcatga
      421 aggaagggtc ggtggccagc gcccgcgtga tctcgactcg ccgccgctcc ccacccgaga
      481 gcgtgtacgc ccggctgttg gccaggtggg cgatgttcaa ttcccgcagc agcgcctcag
      541 tcctccccgc ccgttccggc gcgggcagac ccaccgcctc caggatcgcc aggatgtttt
      601 cctcgaccgt cagcttgcga aagatggacg gctcttgcgg gaggaatccc atgcctcggc
      661 gcgcccgctg gtggacgggg aggaaggtga cgtcgtcgcc gttcaagaac acccgccccg
      721 aatcgggacg ggccagcccc agaaccatgt agaaggccgt ggtcttgccg gcaccgttgg
      781 gccccagcag ccccacgacc tcgcctgcgt ccagtgagag actgaggtcc gacaccacga
      841 cccgctgctt gaagctcttg accagcttct ccgtcttgag agaccgctcc atcacgtggt
      901 catcaccgcc gccgtggggt cagcggcgcg tgggggcgcg ctccggcccc gcgccccgtt
      961 tcgggtagag caccgcgttg acccgctctc cctcgcaacc gtcgacctgg ctccggtcct
     1021 cgcgcatgag caacgtgatc ttgcagccgg tgaccacgtc actgtcctgc cacacccgag
     1081 ggtgcccgag caggaccgcc cgattctccc cggggtagta ctcggcccgc tgggccacgc
     1141 cacgccgttc gccgtaggcg atgcggacgt tgcccgtggc cagcgcccgc tccacatcct
     1201 ccatcttctc gtcgaagaag aattcgatct gatccgcttg gatgctcagg ccgcgcccct
     1261 tgtcactggc ggccacgctc ccccgataga tcgccacccg atctttgcgg ttgacctcca
     1321 gcgagtcggc cgagatgctc agaggtgtcc gttcctccgc cgcccggagg gtccccggca
     1381 tgaccagaag caggacgttg aagagcagca accatcgcac gggcgtcatg gcgcgacaat
     1441 ccttcccgcc ggcatgggcg tgtctcgggc ggcagcgggc gcgatctgcg aggtgatgtt
     1501 ttggaggatc cgaactcgct ccagactcgt
//
LOCUS       DBNGZC010000847         4231 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1443244, whole genome shotgun sequence.
ACCESSION   DBNGZC010000847 DBNGZC010000000
VERSION     DBNGZC010000847.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1443244"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /gene="hypD"
                     /locus_tag="AC16G0_14600"
     CDS             complement(<1..925)
                     /gene="hypD"
                     /locus_tag="AC16G0_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HIR5100881.1"
                     /translation="MKFIDEYRDAAVARRYAAAIAQAVTRPWTIMEICGGQTHSIVKY
                     GVDTLLPASITLVHGPGCPVCVTPVEMLDKAMEIASRPEVIFCSFGDMLRVPGTERDL
                     LVVKAGGGDVRIVYSPLDAVTVARENPDRQVVFFAVGFETTAPANAMAVAHAKRQGLT
                     NFSVLVSHVLVPPAMEAILGSPGNRVQGFLAAGHVCSVMGYTEYEPIAQKYRVPIVVT
                     GFEPVDILQGIFMCVRQLEDGRVEVENQYARSVRREGNQPAIQLMREVFRVRPRKWRG
                     IGEIPESGLGLSEAYAAFDAESRFGVAEYTRD"
     gene            complement(922..1176)
                     /locus_tag="AC16G0_14605"
     CDS             complement(922..1176)
                     /locus_tag="AC16G0_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HIR5100882.1"
                     /translation="MCLAVPGKVMGITGDDPLMRTGKVSFGGIVKEVNLACVPEVKVG
                     DYVIVHAGLAISILDEQEAAITLEYLDQIDLLGRETQGSA"
     gene            1468..1875
                     /locus_tag="AC16G0_14610"
     CDS             1468..1875
                     /locus_tag="AC16G0_14610"
                     /inference="COORDINATES: protein motif:HMM:NF023395.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR5100883.1"
                     /translation="MECVFCAIRDGRLPASRVAESDRAFAIMDINPLSDGHTLIIPTT
                     HSKSLFEIGEEDLLGVARLTRRVAAGIRKGLNPDGLSLIQANGAAAFQSVPHFHLHLI
                     PRWHQDGVGFDWRLVQGDPDRIRAVAQRIRAAL"
     gene            2077..2940
                     /gene="accD"
                     /locus_tag="AC16G0_14615"
     CDS             2077..2940
                     /gene="accD"
                     /locus_tag="AC16G0_14615"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIR5100884.1"
                     /translation="MAWFKKEPLVSGKEGKTRIPEGLWVKCEGCKEIIYKQDLDQNLN
                     VCPRCQHHYRIGAKARVDLLVDAGSFREEDAGLRSLDPLNFKDLKGYPERIKTAKKAT
                     GMEEALLSGTGMIGGHRVSLAILEFGFMGGSMGSVVGEKVTRAIERALAERVPFIAVS
                     CSGGARMQEGVLSLMQMAKTSAALGRLGRAGLPYVSILTDPTTGGVTASYAMLGDILI
                     AEPKALIGFAGPRVIQDTIRQELPPGFQRAEFLLDHGFVDMIVERKNLRPVLIQLLDF
                     LAGTPVDAARS"
     gene            3014..>4231
                     /locus_tag="AC16G0_14620"
     CDS             3014..>4231
                     /locus_tag="AC16G0_14620"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR5100885.1"
                     /translation="MTYTEATQFLFGLRRFGWRPGLATVRHLLELLGNPQRTIPSLHV
                     SGTNGKGSTAAMLSAILRAAGYRTGLYTSPHLLDFTERIRVNGTPISPAEVTELTASL
                     REVCAAHFDPKPGPNPPDESLPYPTFFELTTAMAFLHFATQQVEAAVIEVGLGGRLDA
                     TNVITPQVAVVTSISLEHQEYLGRTVREIAGEKAGIIKPGVPIVTATSGEALSVIRRV
                     ANEQRAALVSVREEYAWTIRESGLDGQVFDVAGPTRRYEAMQLSLAGRHQIENAVTAI
                     AASERLARLGFRMDETAIRRGLVEMRWPGRLQFVWQRPRILLDGAHNPAGAEALAAFL
                     TEHRDDLHRLILVFGVLRDKDWEAMLSLLGPLADQTILTHPPTDRGADPRELVTADHH
                     CRKLAIATEPGEGL"
BASE COUNT          725 a         1381 c         1382 g          743 t
ORIGIN      
        1 cgtcccgcgt gtactccgcc accccgaagc ggctctcggc gtcgaaggcc gcatacgcct
       61 cgctgagtcc caggccgctt tccgggatct ctccgatccc ccgccacttg cgcgggcgga
      121 cccggaacac ctcgcgcatg agctggatgg cgggttggtt cccctcgcgt cgaaccgagc
      181 gggcatactg attttcgacc tcgacccgtc cgtcctccag ttgccggacg cacatgaaga
      241 tgccctgcag gatgtccacc ggctcgaagc ctgtcacgac gatggggacc cggtacttct
      301 gggcgatcgg ctcgtactcg gtgtagccca tgaccgagca gacgtgtccc gccgccagga
      361 agccctgcac gcggttcccg ggggatccca gaatggcttc catggcggga gggacgagga
      421 cgtgggagac caggacggag aagttggtga ggccctggcg cttggcgtgc gccaccgcca
      481 tggcgttggc cggggcggtg gtctcgaaac ccacggcgaa gaacaccact tgccggtcag
      541 ggttctcacg agcgaccgtg acggcgtcca gaggggagta gacgatgcgc acgtcgccac
      601 cgccggcctt gaccaccagg agatcgcgtt ccgtgccggg gacccggagc atgtccccga
      661 acgagcaaaa gatcacttcg ggacgcgagg cgatctccat ggccttgtcc agcatctcca
      721 cgggcgtgac acagacgggg cagccgggcc catgaaccag ggtgatggac gcagggagga
      781 gggtgtccac gccgtacttg acgatggagt gcgtctggcc gccgcagatc tccatgatgg
      841 tccaggggcg cgtcaccgcc tgtgcaatgg ctgcggcgta tcgcctggca acagcggcat
      901 cgcggtattc gtcgataaac ttcatgccga cccctgcgtc tcacggccca gcaaatcgat
      961 ctgatccaga tactccagtg tgatggcggc ttcttgctcg tccagaatgc tgatggcgag
     1021 cccggcgtgc acgatgacat agtcgccgac cttcacctcg gggacacagg cgaggttgac
     1081 ctctttgacg atcccgccga agctcacctt ccccgtgcgc atgagcgggt catcgcccgt
     1141 gatgcccatg accttccctg gaactgctag acacattgag cttcctccct tactcagtag
     1201 cgcgcagact gtcgtctatc ttagtgcttc tggcccaaag taaggtgacg tattgccggt
     1261 ccccccgacc gcccctcgga cgcctggcgc ctcgtcgatg ccggctcgcc cttaccccgg
     1321 gtcggccgcc acagaacggc ggcctccgtg ccggggacgg gctcgcctgc gggcgcggcg
     1381 tctcgggtcg gttcagggga ccgggacttc tctaggcaaa gtttacgtca tcgaatttac
     1441 gtccacgagg cctaagggaa gggacgcatg gagtgcgtgt tctgcgccat ccgtgatggg
     1501 cggctccccg cgtcgcgggt ggccgagagt gatcgcgcct tcgccatcat ggacatcaat
     1561 cccttgagcg atggccacac cctcatcatc cccacgaccc attccaagag tctgtttgaa
     1621 atcggcgagg aggatctgct gggcgtggcc aggctgaccc ggcgcgtggc tgcgggcatc
     1681 cgaaaggggc tcaacccgga cggcctcagt ctgatccagg ccaacggggc tgccgccttc
     1741 caatccgtgc cccatttcca cctccacctg atcccgcgtt ggcaccagga cggtgtcggc
     1801 tttgactgga ggcttgtcca gggagacccc gaccggatcc gcgcggtagc ccagaggatc
     1861 agggcagccc tctgagcccg atagcttcac cgcgaagacc gctgacatcc gccagattcc
     1921 atcaatcaag gagatcatct ggcggtcttc gcgcattgat ggcgaggccg ttgaaatcgg
     1981 tgcttgagct gcactctcta ggctagggtg aagcccgccc cggcttgaca gggcgggagg
     2041 cgtgggctac cgtacgctgg cgttacggag gcgcgcatgg cctggttcaa gaaagaaccc
     2101 ttggtaagcg gcaaggaggg gaaaacccgg ataccagagg ggctctgggt caagtgtgag
     2161 ggctgcaagg agatcatcta caagcaggat ctggaccaga atctgaatgt ctgcccgcgc
     2221 tgccagcacc attatcggat tggggccaag gcccgagtgg acctcttggt ggacgcggga
     2281 agcttccggg aggaagacgc aggccttcgt tccctcgacc ccctgaactt caaggacctc
     2341 aagggctatc ccgaacggat caagaccgcc aagaaggcta ccggcatgga ggaggcccta
     2401 ttatcgggga cggggatgat cggggggcac cgggtgagtc ttgcgatcct ggagttcggc
     2461 ttcatggggg gcagcatggg gtcagtcgtg ggagagaaag tcacccgggc catcgaacgg
     2521 gcgctcgcag agcgggtgcc tttcatcgcc gtttcctgct ccgggggcgc ccgaatgcag
     2581 gagggggtgc tctcgctcat gcagatggcc aagaccagcg cggcgctggg ccgactcgga
     2641 cgggcgggct tgccgtacgt ctcgatcctg acggatccga cgaccggcgg ggtcactgcg
     2701 agctacgcca tgttggggga catcctcatc gccgagccga aggccctcat cggctttgcg
     2761 gggcctcggg tcatccagga tacgatccgc caggaattgc cccccgggtt ccagcgggca
     2821 gagtttctcc tcgaccacgg ctttgtggac atgatcgtgg aacggaagaa tctgcgcccg
     2881 gtactgattc agctcctgga ttttcttgcg gggacgccgg tagacgccgc acgctcatag
     2941 accggccgtt ctactcggcc tcgggtcccg caccccgcct gcgcggcctc cttcggatcc
     3001 agggatggct tccgtgacct acactgaggc gacccagttc ctcttcggcc tgcgccgctt
     3061 cgggtggcgc ccgggccttg cgaccgtgcg gcacctcctg gaactcctgg gcaaccccca
     3121 gcggaccatc ccgagtctgc acgtctccgg caccaacggc aagggctcca ccgcagccat
     3181 gctcagcgcg atccttcggg cggccggcta caggacgggc ctgtacacct cgccgcacct
     3241 cctggacttc acagaacgga ttcgcgtaaa cgggacgccc atcagcccgg cggaggtcac
     3301 ggagctcacg gcgagcttgc gggaggtgtg cgcggcccat ttcgatccga agccgggccc
     3361 gaatcccccc gatgagagcc tcccctatcc caccttcttc gagctgacga ccgccatggc
     3421 gtttctgcat ttcgccaccc agcaggtcga ggccgccgtg atcgaggtag gtctgggcgg
     3481 ccggctggat gcgaccaacg tgatcacccc gcaggtcgcg gtggtgacca gcatctccct
     3541 ggagcatcag gagtacctgg gccggacggt ccgggagatc gccggggaga aggcgggcat
     3601 tatcaagccc ggcgttccca tcgtcaccgc gacatccggc gaggctttgt cggtcattcg
     3661 tcgcgtggcg aacgagcaac gggccgcgct ggtttccgtc agggaggagt acgcgtggac
     3721 gattcgggaa tcgggtcttg acggtcaggt ctttgatgtg gcgggcccca cccgccggta
     3781 cgaggccatg caactctcgc tggccggtcg ccatcagatc gagaacgccg tcaccgccat
     3841 cgccgcctcc gagaggctgg cgaggctagg gttccggatg gacgaaacgg cgatccgccg
     3901 cggtctcgtc gagatgcgct ggcccggcag gctacagttt gtgtggcaac ggcctcgcat
     3961 cctcttggat ggggcccaca atcccgccgg ggctgaggcc ctggcggcat tcctgacaga
     4021 gcatcgagat gacctgcatc ggctcatcct ggttttcggg gtcctccgcg acaaggactg
     4081 ggaggccatg ctgagtctcc ttggcccgtt ggccgatcag accatcctca cccacccgcc
     4141 cacggaccgg ggtgccgatc cgcgtgagct ggtcacggcc gaccaccact gccgcaagct
     4201 ggccatcgcg acggagcctg gtgaaggcct c
//
LOCUS       DBNGZC010000848         4852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_757204, whole genome shotgun sequence.
ACCESSION   DBNGZC010000848 DBNGZC010000000
VERSION     DBNGZC010000848.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_757204"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..422
                     /locus_tag="AC16G0_14625"
     CDS             39..422
                     /locus_tag="AC16G0_14625"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:114..116,aa:Sec)
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HIR5100886.1"
                     /translation="MLDGRAISLSEFKGKPVVVNFWHSGUPHCQREAPVLAAAYEKYK
                     DKGVVFVGVNVPWDKDQLARMFVAVYKVPYPVGRDASGTIAGLYTVEATPTTFFIGKN
                     GKVLERVEGEMESPDVTKHIEALLK"
     gene            complement(519..890)
                     /gene="mnhG"
                     /locus_tag="AC16G0_14630"
     CDS             complement(519..890)
                     /gene="mnhG"
                     /locus_tag="AC16G0_14630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HIR5100887.1"
                     /translation="MPSLHELLVAALLLTGTFLILLTGIGLIRFPDVFCRMHAAGKAG
                     TLGVIFLVLGTVITFAGTDQDLWLRGVLAVFFQLITAPAATHLLARACYVCDYPLSER
                     TAVDELRIFLPSRPDEVFNQE"
     gene            complement(890..1165)
                     /locus_tag="AC16G0_14635"
     CDS             complement(890..1165)
                     /locus_tag="AC16G0_14635"
                     /inference="COORDINATES: protein motif:HMM:NF015993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MrpF/PhaF family protein"
                     /protein_id="HIR5100888.1"
                     /translation="MSPFLAIVLKLCLAGLLASGLMCLYRMFIGPKAADRAVAFDTLA
                     MIFIGVICVLCIQWHSVLYFDAVWILMLVGFLGSASIAKYLEKGRIF"
     gene            complement(1176..1508)
                     /locus_tag="AC16G0_14640"
     CDS             complement(1176..1508)
                     /locus_tag="AC16G0_14640"
                     /inference="COORDINATES: protein motif:HMM:NF014012.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HIR5100889.1"
                     /translation="MLRRVAFAVIYFFKFMKELALANYQVAKLVLSPAVKLKPGFVSI
                     PLDARTDFEITSFANSITLTPGTISVYVPEDRHAIVIHAIDVGDDVDALRRATKDALE
                     SPILRFTR"
     gene            complement(1508..3079)
                     /locus_tag="AC16G0_14645"
     CDS             complement(1508..3079)
                     /locus_tag="AC16G0_14645"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter transmembrane
                     domain-containing protein"
                     /protein_id="HIR5100890.1"
                     /translation="MNSLILSILVPFLTALLLALLNGHYRLEKAVALLSSLGLTVWVL
                     WLLFYVDAHGVQVVIIGGWMAPWGIAFVADRLTAIMLCLSTGLGTLVQLYSFWTVTEQ
                     QQRYFFYPLMQAMLLGVNWSFITGDVFNLFVAYEVMLMASYGVMMVGASPVQVRQTLK
                     YIAINSIGSTLFVVGCGVVYATVGTLNMADLAVRTALITDERAGMVTAASMLLLLVFG
                     LKAATFPLIYWLPDSYPVVPPGVNGYFAGILTKVGVYSLLRVFVLCFRQEGHQFALDV
                     LLFLSGLTMLLGVLGAMCRWDIRRILSWHIISQVGYMVMGIGLAGSPDPRVVQLAVAG
                     TIFYVVHHIVVKSCLFLVGGIAERVSGSQDLKHMGGVLGLAPGVGGLFIVVALSLAGM
                     PPFSGFLSKFVLAQAGLMGGNYGVVTIAVITSFFTLYSMSKIWSYAFWRESSRKRPAL
                     TYRGMMTPTAVLVAFTIAMGLFAQPFLGLASRAAETLTRPDEYIRTVLTARQNRLASL
                     PHSAAAALAQLEEGR"
     gene            complement(3076..3570)
                     /locus_tag="AC16G0_14650"
     CDS             complement(3076..3570)
                     /locus_tag="AC16G0_14650"
                     /inference="COORDINATES: protein motif:HMM:NF012636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HIR5100891.1"
                     /translation="MELPMAAVIAVLFAAGTYLILRPNLIRLVMGFGLYSNATNLLLI
                     TVGGYTRTSGAPFVGADPKTAAGLMDPLPPDIILTAIVISFAVSALMLTICYRVYQDH
                     GTDNPQELPVDDDFGSTAEMQLDASARDDGHGQIHGALQVLPVGGGMPAAGDGQSGRK
                     AGRR"
     gene            complement(3570..3983)
                     /locus_tag="AC16G0_14655"
     CDS             complement(3570..3983)
                     /locus_tag="AC16G0_14655"
                     /inference="COORDINATES: protein motif:HMM:NF015970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HIR5100892.1"
                     /translation="MRGDPILRTVSRFAVPLTVWVSLVIFMQGHNLPGGGFIAGVMAA
                     AAGAMYLLAFGPAGATRYRWWKVSVFGLLVSVTTGTVPFLMGRPYMQHAIWHFDLPVI
                     GPYELPTATFFDLGVYLIVLGTIMTIFVELAQEGH"
     gene            complement(3980..>4852)
                     /gene="mbhE"
                     /locus_tag="AC16G0_14660"
     CDS             complement(3980..>4852)
                     /gene="mbhE"
                     /locus_tag="AC16G0_14660"
                     /inference="COORDINATES: protein motif:HMM:NF042818.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen gas-evolving membrane-bound hydrogenase
                     subunit E"
                     /protein_id="HIR5100893.1"
                     /translation="RFSSDPRAEVHLSLLPTELGPVLLSLITVALGVGIYVARPAFAR
                     WRERLQGLPSMQSLWDGLMDAVTAAAIAFSVRWQSGSLRWYLGGCLAFTVGLSGLALG
                     HAGISILQVPISLHEMTWYGLTLCTMLGMTALLVARSSTRIGSAIALTSNGFLTALLY
                     VVYRSPDILLTQILIETVSTIFLLLILYYMPTFRAEGLPPMRRLINLAMSVAVGLAMF
                     SFIVLSTSPQFREVKNLAAAYLTRSMAEAGGANAVNVIIVDFRAMDTNGEITVLVLVG
                     LLVFGLLRSRRKAA"
BASE COUNT         1000 a         1481 c         1659 g          712 t
ORIGIN      
        1 cgacggtcgg gcagacggcg cctctcttca gcctgacctt gctcgacggc cgggcgattt
       61 ccctcagcga gttcaaaggg aaaccggtcg tggtgaactt ctggcattcg gggtgaccgc
      121 actgtcaacg ggaggctccc gttctggcgg cggcctacga gaagtacaag gacaagggtg
      181 tggtgttcgt gggcgtgaat gttccctggg acaaggatca actggcccgg atgttcgtcg
      241 cggtgtataa ggtgccgtac ccggtcggcc gcgatgcctc cgggacgatc gccgggctgt
      301 acacggtgga ggccacgccc accacgttct tcatcgggaa gaatggaaaa gtgctggagc
      361 gggtcgaggg agagatggaa tcccccgatg tcaccaagca catcgaggcg ctgctgaagt
      421 agtcggcgga ctcatgacga aaaacggggt actccggaaa tggagcaccc cgttttcttt
      481 ttggccacgg gaggtatggc ggagtccggc ggggccgatc actcctggtt gaacacctcg
      541 tccggcctcg agggcaggaa tatccgcagc tcgtccaccg cggtccgctc cgacagcggg
      601 tagtcgcaga catagcaggc ccgcgccagc aggtgggtgg cggctggcgc cgtgatcaac
      661 tggaagaaga cggcgaggac gccccgcagc cagaggtcct gatcggtccc ggcgaaggtg
      721 atgacggtcc ccaagaccag gaagatgacc ccgagcgtgc cggccttgcc cgcggcgtgc
      781 atgcggcaga acacatcggg aaaacggatc agaccgatgc ccgtcagcag gatgaggaac
      841 gtgcccgtca gcagcaatgc ggcgacgagg agttcgtgga ggcttggcat tagaagatcc
      901 gccccttttc cagatacttt gcgatggaag ccgaacccag gaatcccacc agcatgagga
      961 tccacacggc gtcgaagtac aggaccgagt gccactggat gcagagcacg cagatgacgc
     1021 cgatgaagat catggccagc gtgtcgaagg ccacggcccg gtcggcggcc ttggggccga
     1081 tgaacatccg gtacaggcac atgaggccgc tggccagcag gcccgccaag cacagcttca
     1141 agacgatggc gagaaatggg ctcatggcat ccgtcctagc gcgtgaagcg cagaatgggg
     1201 gattccaagg cgtccttggt ggcccgccga agggcgtcca cgtcatcccc cacgtcgatg
     1261 gcgtggatca caatggcatg ccgatcctcc ggaacgtaga cggagatcgt gccgggagtg
     1321 agggtgatgg agttggcgaa gctggtgatc tcgaagtccg tcctggcatc cagggggatt
     1381 gagacgaatc ccggcttgag cttgacggcc ggggacagga ccagcttggc cacctggtag
     1441 ttggccaggg ccaactcctt catgaacttg aagaagtaga tcacggcgaa tgcgacacga
     1501 cgcagcatca gcgaccctcc tcgagctggg ccagggcggc ggccgcggag tggggtaggc
     1561 tggccaagcg gttttgcctg gcggtcagaa ccgtccggat gtactcatcg ggtcgcgtca
     1621 gtgtctccgc ggcccgagac gccaggccga ggaaaggctg ggcgaacaga cccatggcga
     1681 tggtgaaggc cacgagcacc gccgttggcg tcatcatgcc ccgataggtg agggctggcc
     1741 gcttccggct cgactcccgc cagaaggcgt aggaccagat cttgctcatg gagtagaggg
     1801 tgaaaaagct ggtgatcacc gcgatggtga ccaccccgta gtttccgccc atcaggcccg
     1861 cctgggccag cacgaacttg ctcaggaagc ccgagaaagg cggcatcccg gccaaggaca
     1921 aggcgaccac gatgaacagg ccgcccacgc cgggcgccag gcccaggacg ccccccatgt
     1981 gcttgagatc ctgggagccg ctcacccgct cggcgatccc gcccacgagg aagaggcagg
     2041 acttcaccac gatgtggtgg accacgtaga agatcgtgcc tgccaccgcc agctgcacca
     2101 cccgcgggtc cggactcccc gccaggccga ttcccatgac catgtagccc acctggctga
     2161 tgatgtgcca ggaaaggatt cgccggatgt cccagcgaca catggccccg agcaccccca
     2221 agagcatcgt gaggccggag aggaagagga ggacgtccag ggcgaactga tgcccctctt
     2281 gccggaagca gaggacgaag acgcggagca gcgagtagac gcccaccttg gtcaggatgc
     2341 ccgcgaagta cccgttgaca cccgggggca ccaccggata ggaatcgggc agccagtaga
     2401 tgagggggaa ggtggcggcc ttcaagccga acaccagcag gaggagcatg gaggcggcgg
     2461 tgaccatgcc ggcccgctcg tcggtgatca gagcggtgcg caccgccagg tcggccatgt
     2521 tcaacgtccc cacggtggcg tacaccaccc cgcagccgac cacgaagagc gtcgatccga
     2581 tgctgttgat ggcgatgtac ttcagggtct ggcggacctg gacggggctg gcgcccacca
     2641 tcatgacccc gtacgaggcc atcagcatca cctcataggc cacgaacagg ttgaacacgt
     2701 cgccggtgat gaaggaccag ttgacgccga gaagcatggc ctgcatcaag ggatagaaga
     2761 agtaccgctg ctgctgctcc gtcaccgtcc agaaagagta gagctgcacc agggttccca
     2821 gcccggtgga caggcagagc atgatggcgg tcaggcgatc cgccacgaag gcgatgcccc
     2881 agggggccat ccagccgccg atgatcacga cctgcacgcc gtgggcgtcc acatagaaca
     2941 gcagccagag gacccagacg gtcaggccga ggctggagag caatgccacg gccttctcca
     3001 gccggtagtg gccgttcagg agggccaaga ggagggcggt gaggaagggc accaggatgg
     3061 acaggatcag gctattcatc ggcgccccgc ctttcgcccg gactgcccat cccccgctgc
     3121 aggcatgcct cctcccaccg ggaggacctg gagcgcgccg tggatctgcc cgtgcccgtc
     3181 atcgcgagcc gacgcgtcca gctgcatctc cgccgtcgag ccgaagtcgt cgtccacggg
     3241 aagctcctga ggattgtccg tgccgtggtc ctgatagacc cgataacaga tcgtcagcat
     3301 cagggcgctg accgcaaagc tgatgacgat ggccgtcaag atgatgtccg gcggcagggg
     3361 atccatgagt cccgcggctg tcttgggatc ggcccccacg aagggtgcgc cgctggtgcg
     3421 ggtgtacccc ccgacggtga tcagcaggag gttggtggca ttcgagtaca ggccgaagcc
     3481 catgaccagc cggatcaggt tcggacgcaa gatcaggtag gtgccagcgg caaagagcac
     3541 cgcgatgacc gccgccatcg gcagttccat cagtgtccct cctgggccag ctcgacgaag
     3601 atggtcatga tcgtccccag cacgatcagg tagacgccga ggtcgaagaa ggttgcggtg
     3661 ggcagctcgt acgggccaat gaccggcaga tcgaaatgcc agatggcgtg ctgcatgtag
     3721 gggcgtccca tgaggaaggg gacggtgccc gttgtcaccg agaccaggag cccgaagacc
     3781 gacaccttcc accagcgata tcgagtcgcc ccggctggcc cgaaggccag gaggtacatg
     3841 gcccccgcgg ctgccgccat gacgccggcg atgaatccgc caccgggcaa gttgtgccct
     3901 tgcatgaaga tcaccaggga cacccacacg gtgaggggca cggcgaaacg cgacactgtc
     3961 cggagaattg gatcgcctct catgccgcct tcctccgcga tcgcagaagc ccgaacacca
     4021 gcaggcccac caggaccagc acggtgattt ccccgttggt gtccatggcc cggaagtcca
     4081 cgatgatgac attgaccgcg ttggcgccgc ctgcctcggc catggagcgt gtcaggtagg
     4141 cggcggccag gttcttgacc tcccggaact ggggactggt gctgagcacg atgaagctga
     4201 acatggcgag gcccacggcc accgacatgg ccaggttgat tagccgccgc atgggcggga
     4261 gcccctcggc ccggaaggtg ggcatgtagt acaggatgag gagcaggaag atggtggaga
     4321 cggtctcgat caggatctga gtgagcagga tgtcgggcga ccggtagacc acgtagagca
     4381 gggccgtgag aaagccgttg gaggtgaggg cgatggccga gccgattcgc gtcgaggacc
     4441 gcgccaccag cagggccgtc atccccagca tggtgcagag cgtgagtccg taccaggtca
     4501 tctcgtgcag gctgatgggg acctggagga tggagatccc ggcgtgaccg agggcgaggc
     4561 cggagagccc cacagtgaag gccagacatc cccccaggta ccagcgcagc gacccgctct
     4621 gccagcggac gctgaacgcg atggcggccg cggtcaccgc gtccataaga ccgtcccaaa
     4681 ggctttgcat gctcggcagg ccttggagtc gctcgcgcca gcgggcgaag gcgggccgcg
     4741 cgacgtagat ccccacgccc agggcgacgg tgatcaggct caggagcacc ggccccagct
     4801 cggtcgggag cagggaaagg tgaacctcgg cccgcgggtc ggaggaaaaa cg
//
LOCUS       DBNGZC010000849         1945 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2128008, whole genome shotgun sequence.
ACCESSION   DBNGZC010000849 DBNGZC010000000
VERSION     DBNGZC010000849.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2128008"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="AC16G0_14665"
     CDS             complement(<1..567)
                     /locus_tag="AC16G0_14665"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5100894.1"
                     /translation="MSALSRLLRHRRGTVGGVAIGVMVMTGALAPVIAPYSYSTQSLL
                     QRLQPPGGLHWLGTDGFGRDVLSRIIWGSRVSLEIGFLATGLALVIGIALGSAAGHFG
                     GAADTVSMRFADVFMSVPALFLILIVVALFGASLLNTGLVIAFVSWAPVARVVRSECL
                     SLREREFVRAAHALGASQARVLGQHLLRN"
     gene            complement(597..1559)
                     /locus_tag="AC16G0_14670"
     CDS             complement(597..1559)
                     /locus_tag="AC16G0_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007071436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5100895.1"
                     /translation="MEAGLRSYVLKRVSFAALMLFGVSAILFAIMHLAPGGPEAVLVG
                     GEFSPETAAQIRQRLGLDRPLLIQYASWVSAAVRGDLGRSFKTGDPVAALIFERLGPT
                     LQLTAGALGLALAVAIPLGVLAAVRWNSLWDTVGSAIALFGVSFPSFWLGIMLILLFS
                     ETLHLLPPSGLSEYGREADLASRLRHAILPTLTLGLIQMASFMRFTRSSLFEVLRQDY
                     VRTARAKGLSAARVIWRHALRNALIPVVTVVGLSLPTLVGGAVLTETVFAWPGIGRLA
                     VGAVFERDYPIIMGVNLLVAGVVITANLLTDIAYCLIDPRITFK"
BASE COUNT          394 a          611 c          663 g          277 t
ORIGIN      
        1 gttgcggagc aggtgttggc ccagcacacg tgcctggctg gcgccgaggg cgtgggcggc
       61 ccggacgaat tcccgctccc gaagactcag acactcgcta cggacgacac gggcgaccgg
      121 cgcccaggac acgaaggcga tgaccagccc cgtgttgagc aggctcgccc cgaacaacgc
      181 cacgacgatc aggatcagga atagggccgg gaccgacatg aaaacgtcgg cgaaccgcat
      241 gctgacggtg tcggcggctc caccgaaatg gcccgccgca ctgcccaggg cgatcccaat
      301 gacgagggcg agaccggtgg cgaggaagcc gatctcgagg gagacgcggc ttccccagat
      361 gatgcggctg aggacatcgc ggccgaaccc atcggtgccc agccagtgga ggccacccgg
      421 cggctgcaag cgctgcagga gtgactgggt ggagtagctg tacggggcaa tgacgggtgc
      481 caaggccccg gtcatgacca tcaccccgat ggcgacgccc cccaccgtac cccgtcggtg
      541 gcgaaggagg cgcgaaagcg ctgacatgga ttccggacgg accagtaatg gcgacatcac
      601 ttgaaagtga tccggggatc gatgaggcag taggcgatgt ccgtcaggag attggcggtg
      661 atgaccacgc cggccacgag caggttcacc cccatgatga ttggatagtc gcgttcgaag
      721 acggccccga cggccagccg gccgatcccc ggccaggcga agaccgtctc ggtgagcacg
      781 gcgccaccga ccagcgtggg cagcgacaag cccaccacgg tcacgaccgg gatcagcgca
      841 ttgcgcagcg cgtggcgcca gatgacccga gcggcggata ggcctttggc cctggcagtt
      901 cgaacgtaat cttgtcgcag gacttcaaac aggcttgagc gggtgaagcg catgaaggaa
      961 gccatctgga tcaggccgag tgtcagggtc ggaaggatcg cgtggcggag acgcgaggcc
     1021 aaatcggcct cgcggccgta ctcactgagt ccggaaggtg gcagcaggtg gagggtctct
     1081 gaaaaaagca agatgagcat gatcccgagc cagaagctcg ggaacgagac cccgaagagc
     1141 gcgatcgccg atccgacggt gtcccacagc gaattccacc tgacggcggc caagacgccg
     1201 agggggatgg cgacggcgag agccagaccc aaggcacccg ccgtcagttg tagcgtggga
     1261 cccagacgct caaagatgag ggcggccacg ggatcgccgg tcttgaagga tcgcccgaga
     1321 tcgccccgga ccgctgcgga cacccaggat gcgtattgga tgaggagtgg ccgatccagg
     1381 ccaagacgct ggcggatctg ggccgccgtc tctggtgaaa actcgccccc gacgagaacc
     1441 gcctccggcc cccccggcgc gaggtgcatg atggcgaaca ggatggcgga cacgccgaac
     1501 agcatcaagg cggcgaagct cacgcgcttc aaaacgtagg atctgagacc cgcttccatc
     1561 ggtggtcgtg ctcggcgcgc acgaggcccc ttctgggtgc cgcggctgcc ttctctattt
     1621 caagtccatg gttttctggt cgcgcgggct gttgcgaatc tgcttctgga tcgggcagcg
     1681 ctgccgctat tgcgggaccg acgcaggccc ggacacaccc tatagacagg cggagcatgt
     1741 cgtagcaagc ggggcgggtc aagccaaaac ccgcgagggg agcggaccgg ctatatggct
     1801 cccgcggtcc cgcgttcccg acctgtttgc cgttgacacg cggcacgccc ttggataggc
     1861 tgcagccagt tctctcgagc cagaaccccg cccgtgctcg agatcgctac ccccgaagaa
     1921 catcgctttg cctgtttcgc attct
//
LOCUS       DBNGZC010000850         1927 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2128011, whole genome shotgun sequence.
ACCESSION   DBNGZC010000850 DBNGZC010000000
VERSION     DBNGZC010000850.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2128011"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..804
                     /locus_tag="AC16G0_14675"
     CDS             127..804
                     /locus_tag="AC16G0_14675"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR5100896.1"
                     /translation="MEKDEFLGRVRLFQDLTPRERRDIQGLFAEQRVERDQYIFLEGD
                     AADYVYVVVEGKVKIVKQAPGGKEVILEVFAATEVFGGATLLLDRHPASAVAMEPGVV
                     LRLPRAHYKALLQRFPPLAFQIIERLADRLREAHQVIRGLAADRVEARMARLLFKLAD
                     KIGAPAGNGTRLQLQLTRQDVADMVGCTLETAIRILSRWQKEGLIKTEEGVITILNRQ
                     ELSRLLT"
     gene            953..>1927
                     /locus_tag="AC16G0_14680"
     CDS             953..>1927
                     /locus_tag="AC16G0_14680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100897.1"
                     /translation="MAFATSPSIRRLEGRAAAIFTNSDTRRLPGRGPTAASNLRFLEW
                     FLQDYSPRRGEGPLLGEFADAVLDLSPREEELLLASLLTPMRAWEVTEVLGLRGFLLK
                     DLLTGAESRVTSLGLAELAISSDILICRLLPWGGIMRPGISLIRLPAVSREEMLAYLR
                     TAYQMARPARHVSLEDFLDGAAHLYHHFFALRGKTLGGRTQESVRRTTFAPGKFTYRG
                     KDTLRIRAGLNRQMQLELVGDTGDEVRYALVDLDRAIIRASVLLRPGAVEVSAETRED
                     LAEAGRFVEACLRGLIQPVEPHPESLSSPSPSPDRHLEGRRRGAAFLVG"
BASE COUNT          299 a          631 c          659 g          338 t
ORIGIN      
        1 cctggccggt ctggctgttg ccgaggcgat cggcatctcg gccgaacggc cgccggccct
       61 tgaggcgaat ggctctgctg tccaggaagc ggcggagaca ccccgccagg atacgtgagc
      121 gggcggatgg aaaaggacga gttcctcggt cgggtgcggc tgtttcagga cctgacgccg
      181 cgcgagcggc gggacatcca agggcttttc gcggagcagc gcgtcgagcg ggatcagtat
      241 atcttcctgg agggggatgc cgccgattac gtctatgtcg ttgtcgaggg gaaggtgaag
      301 attgtgaaac aggcccccgg cggcaaggag gtgatcctgg aggtctttgc cgccacggag
      361 gtgttcggcg gcgcgacatt gctcctggat cgccatcccg cctcggcggt ggccatggag
      421 ccgggcgtgg tccttcgcct cccgcgcgcg cactacaagg cgctcctgca gcgattccct
      481 cccctggcat ttcagatcat cgagcggttg gccgaccgat tgcgggaggc ccaccaggtg
      541 atccgcgggc ttgcggctga ccgcgtggag gcccggatgg ctcgcctgtt gttcaagctg
      601 gcggacaaga tcggcgcgcc cgccgggaac gggacgcggc tgcagcttca actgacgcgt
      661 caggacgtcg ccgacatggt gggttgcacc ctggagaccg ccatccggat cctgagccgc
      721 tggcagaagg agggcctcat caagaccgaa gagggtgtaa tcacaatcct gaaccggcag
      781 gagctgagcc gcctcctcac gtagcgctgg aggcctgacg attggaggtc agaccgtgtc
      841 gccgcgtccc caccgccaac gtcatgagcc gtgtctctgc gggagcggcc gccccctcat
      901 ggcctgttgc cttccctggg aggaagcctt tcagcgtctg atcgctcgcc tcgtggcctt
      961 cgcgacatcg ccgagcatcc ggagactcga gggccgcgcc gcggccatct tcacgaacag
     1021 cgatacacgg cggctgcccg gcaggggacc gaccgctgcc agcaacctgc gcttcctgga
     1081 gtggttcctc caagactact ctccccgccg cggggaaggg cccttgctgg gcgagttcgc
     1141 ggatgcggtg ctcgatctca gtccccgcga ggaggaactg ctgctggcct ctcttctcac
     1201 gccgatgcgc gcctgggagg tgacggaagt cttgggtctc cggggcttcc tgctcaagga
     1261 cctcctcacg ggagcagaga gccgtgtgac ctccctgggc ctcgccgaat tggccattag
     1321 ctccgacatc ctgatctgtc gtctgctgcc ttgggggggg atcatgcgac cgggcatcag
     1381 cctgatccgg ctgccggccg tgagccggga ggagatgctt gcctacctcc gcaccgcgta
     1441 tcagatggcg cgtccggcgc gtcatgtctc cctggaagac ttcctggacg gcgccgccca
     1501 cctgtaccat cacttctttg ccctccgggg gaaaactctc gggggccgca cccaggagtc
     1561 ggtgcggcgg acgacctttg cgccaggcaa attcacctac cgtggaaagg acaccctccg
     1621 gattcgggcc ggtctgaatc gccaaatgca gctcgagctg gttggcgaca cgggcgacga
     1681 ggtccgctac gcgctggtgg atctcgaccg ggccatcatc cgcgccagcg tgctgctgcg
     1741 acccggcgcg gttgaggtga gcgccgagac gcgcgaagac ctggccgagg cgggacggtt
     1801 cgtggaggcc tgccttcgtg gcctgatcca accggtcgag ccgcatcctg aaagcctaag
     1861 tagcccttcc ccgtcacccg atcgacacct cgaggggcgc cgccgcggtg cggccttcct
     1921 cgttggg
//
LOCUS       DBNGZC010000851         2755 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2325968, whole genome shotgun sequence.
ACCESSION   DBNGZC010000851 DBNGZC010000000
VERSION     DBNGZC010000851.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2755)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2325968"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            249..650
                     /locus_tag="AC16G0_14685"
     CDS             249..650
                     /locus_tag="AC16G0_14685"
                     /inference="COORDINATES: protein motif:HMM:NF013231.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR5100898.1"
                     /translation="MRAIREEMRRQGTSPLSLPQLRTLAYLHRNPGSCLFHLAKYLGV
                     TRPTASTIVERLVRRRMLSRVDNPLERRRIVLTLTPLGEQYLRRARQSTQRWMATALS
                     RLSSARLRRIMQGVTLLGEPFTGAASDNGRE"
     gene            662..2047
                     /locus_tag="AC16G0_14690"
     CDS             662..2047
                     /locus_tag="AC16G0_14690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5100899.1"
                     /translation="MNRGDLWCLADSDAEAAHQLDAPRRGRAIAGAALLLAATLLGAC
                     SNGAAEKAQAKTPAAREAAPVTIDTVVSKAVPVQIRSVGSVQAYAMVTIKSQVDGEVA
                     RVHFTEGQEVKKGDLLFTLDQRPLEAMLQQAEANLARDTAQLEQAEAAVAQNTAAQKQ
                     AEANLARDTAQHENAMTQVRRYKGLIEEGAISSELYDQVRTTATALEATIQADQAAVT
                     NTRAMIRAAQATVQTAHAAIQADKALVANARVQLGYTTIRAPMEGRTGNLLVRVGSAV
                     KARDDTGAMVVINQTHPIYVSFSIPEQSLSDVKKYLKAGSVRVQALPRGQDGSPAVGQ
                     LTFVNNTVDATTGTIQLKATFPNQEGTLWPGQFADVVLTLTVQPDLLVVPSQAIQTGQ
                     QGQYVYVVRPDSTVESRPVTVGRTLGAVAVVEKGLAAGDKVVTDGQLRLAPGSRVQVK
                     TPAASASGQAQ"
     gene            2044..>2755
                     /locus_tag="AC16G0_14695"
     CDS             2044..>2755
                     /locus_tag="AC16G0_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5100900.1"
                     /translation="MNIPELFIRRPVMTTLVMAAILIFGFMGYRLLPVSDLPNVDFPT
                     IQVSAKLPGASPETMASSVATPLEKQFSTIAGIDNMTSNSQQGAIRITLQFALDRNID
                     AAAQDVQSAIAASLRQLPANMPSPPTMRKVNPADWPVFFLSLSSPTLPLSVVDEYAET
                     LLAQRISMVSGVSQVQVHGSQKYAVRVQVDPSAMAARGIGIDEVENAIAEGNVNLPTG
                     NLDGRHQTFAVEANGQLFD"
BASE COUNT          544 a          927 c          858 g          426 t
ORIGIN      
        1 cctgacgcca accgtcaaca gcatgactcc aaaatgagtg tgatgatcta ccgcacaaac
       61 cagaagaggc cattgacagc gatccaggca actgacacaa ttagtctacc taactatcag
      121 caccacatca ctcccatatc gtcatcccga aaggtcgatg gcgatgagta cggcgagagg
      181 tcccagtgac tacagcgaat cttgctgacc agtgtgccag ggttgtgatg gagaccgtcc
      241 ctcaggtcat gcgggcgatc cgcgaggaga tgcgccgaca ggggacctcg cccctttctc
      301 tcccccaact ccggaccctc gcctacctcc atcgcaaccc agggtcttgc ctgttccacc
      361 tggcgaagta cctcggcgtg actcgcccga ccgcctccac catcgtcgaa cggttggtgc
      421 ggcggcgcat gctgagtcgc gtggacaatc cgctggagcg gcggcgcatc gtactcaccc
      481 tcacgcccct gggggagcag tatcttcggc gcgcgcgcca atccacgcag agatggatgg
      541 cgaccgcgct gtcgcggctg tcatcagcca gactccggag aatcatgcaa ggggtgactt
      601 tgctgggaga gcccttcacg ggcgccgcga gtgataatgg ccgggaatga ggagaacgcg
      661 aatgaataga ggggacctct ggtgtctcgc cgatagcgat gccgaggcag cccaccaact
      721 ggatgctccc cggaggggtc gcgccatagc cggggcagcg ctgttgctgg ccgccacact
      781 cctcggggcc tgctccaatg gggcggccga aaaagcccag gccaagacgc ctgcggcgag
      841 ggaggccgcc ccggtgacca ttgacacggt ggtcagcaag gccgttccgg tccagatccg
      901 ctcggtcggc agcgtccagg cgtatgccat ggtgacgatc aagagccagg tggacggtga
      961 ggtcgcccgc gtccatttca ccgaaggaca agaggtcaag aaaggcgacc tcctcttcac
     1021 gctcgaccag cgaccgctcg aggcgatgct gcagcaggcc gaggcaaacc tggcgcgtga
     1081 cacggcgcaa ctcgaacagg ccgaagccgc ggtggcccag aacacggcgg cgcagaagca
     1141 ggccgaggcc aacctggcgc gtgacacggc gcaacacgag aatgccatga cccaggtccg
     1201 ccgctacaag ggtctcatcg aggagggggc catctcgagt gagctgtacg atcaggtgcg
     1261 caccaccgcc acggccctgg aggcgaccat ccaggcggac caggccgcgg tgacaaacac
     1321 ccgggccatg atccgagccg cgcaggccac ggtgcagacc gcccacgcgg ccatccaggc
     1381 cgacaaggcg ctggtggcga atgcgcgggt gcagctcgga tatacgacca ttcgcgctcc
     1441 catggaaggg cgcacgggga acctgctcgt ccgtgtgggc agcgccgtca aggcccgcga
     1501 tgataccggc gcgatggtgg tgatcaatca gactcacccg atctacgtga gcttctccat
     1561 ccccgagcag tctctttcgg acgtcaagaa gtatctcaag gctggaagcg ttcgggtcca
     1621 ggcgctccct cggggccagg acggctcccc ggcagtggga cagctcacct tcgtgaacaa
     1681 cacggtggat gctaccaccg gaacgatcca gctcaaggcc acctttccga accaagaggg
     1741 gaccctctgg ccggggcagt tcgccgacgt cgtgctaacg ctcaccgtcc agcccgacct
     1801 gctggtcgtc ccctcccagg ccatacaaac cggtcagcag ggccagtatg tgtacgtggt
     1861 gcgacccgac tcgacggtgg aatcacggcc cgtcacggtc ggaagaacgc tcggcgccgt
     1921 ggccgttgtg gagaaggggc tcgcagccgg agacaaggtg gtgactgacg gacagctccg
     1981 cctcgcccct ggctcgcgcg tccaggtgaa gacgcccgcg gcgagcgcgt cgggacaagc
     2041 gcaatgaata tccccgagct gttcatacgc cggccggtca tgacgaccct ggtcatggcc
     2101 gccatcctca tattcggctt catgggctac cggctactgc ccgtgagcga tctccccaac
     2161 gtggattttc caaccatcca ggtgtccgcc aagcttccgg gcgccagccc ggaaaccatg
     2221 gcctcctccg tggccacgcc cctggaaaag cagttctcga ccattgctgg catcgacaac
     2281 atgacctcga atagccagca gggcgccatt cggatcacgc tccagtttgc cctggaccgg
     2341 aacatcgatg ccgccgctca agacgtccag tcggccatcg ccgcgagcct gcgccagttg
     2401 ccggcgaaca tgcccagccc gcccaccatg cggaaggtca accccgcgga ttggcccgtc
     2461 ttcttcctgt ccctcagctc gcccaccctg cccctctccg tcgtggacga gtacgccgag
     2521 accctcctgg cccagcgcat ctccatggta agcggcgtct cgcaggtgca agtccacggc
     2581 agccagaagt acgccgtgcg ggtccaggtc gatccgtcgg ccatggcggc gcgggggatc
     2641 gggatcgacg aggtcgagaa cgccattgcg gagggcaacg tcaatctgcc gacaggaaac
     2701 ctggacgggc gtcaccagac ctttgcggtc gaggccaatg ggcagctctt cgacg
//
LOCUS       DBNGZC010000852         1537 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1143856, whole genome shotgun sequence.
ACCESSION   DBNGZC010000852 DBNGZC010000000
VERSION     DBNGZC010000852.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1143856"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /locus_tag="AC16G0_14700"
     CDS             <1..956
                     /locus_tag="AC16G0_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011699795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HIR5100901.1"
                     /translation="RRLEPLAADPAFQREWREVKLENKRRLAVLIEERTGIRVNSESL
                     FDIQVKRIHEYKRQHLNALHILTLYLRLKRDPRADIPPRTFIFGGKAAPGYFMAKRII
                     RLITATAQMVNADPAVHDRLRVVFFPDFNVKSAHHVYPAADLSEQISTAGKEASGTGN
                     MKFSLNGALTIGTLDGANVEIREEVGPENFFLFGLTVDQVHELKARGYRPRDQFDANP
                     MLREVLDFIASGDLSQGDAGLFRPLVDNLLQDDPFLLLADYQAYVDCQERVSALWRDT
                     QAWTRMVILNTARMGKFSSDRSIRDYCERVWHVKPMPVEAS"
     gene            1056..>1537
                     /locus_tag="AC16G0_14705"
     CDS             1056..>1537
                     /locus_tag="AC16G0_14705"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR5100902.1"
                     /translation="MTVDIKRIAINTGGGDAPGLNAVIHATVYAARAVGWETFGIREG
                     YDGLLDPQNYPEGGLVQLTPDMVRHIAHLGGTILGTTNRGNPFKHVVTQPDGTTHELN
                     RSTAVLEAFRKNRLDALIAVGGDGSLAIANQLYEQGLRVVGVHKTIDNDLDATLVTFG
                     L"
BASE COUNT          297 a          490 c          493 g          257 t
ORIGIN      
        1 tccggcggct ggagccgctc gccgccgacc ccgcgttcca gcgggagtgg cgggaggtga
       61 agctggagaa caagcggcgc cttgccgtgc tgatcgaaga gcgaacgggg atccgggtga
      121 actccgagtc gctgttcgac atccaggtca aacgcatcca cgaatacaag cggcagcacc
      181 tcaatgcgct gcacatcctc acgctctatc tgcgactcaa acgcgacccg cgggcggaca
      241 tcccccctcg caccttcatc tttggcggca aagcggcgcc gggctacttc atggccaagc
      301 gcatcatccg gctcatcacg gccacggccc agatggtgaa cgccgatccc gccgtgcacg
      361 accggctgcg ggtcgtgttc ttccccgatt tcaacgtgaa aagtgcgcac cacgtctatc
      421 ccgccgcgga tctgtccgag cagatctcca cggcaggcaa ggaggcctcg gggacgggca
      481 acatgaaatt ctcactcaac ggggccctga ccatcggcac gctggacggc gccaacgtgg
      541 agattcgcga ggaggtgggc ccggagaact ttttcctgtt cggcctgacc gtcgaccagg
      601 tgcatgaact aaaggcgcgg ggctatcggc cgcgcgatca gttcgacgcg aatccgatgc
      661 tccgcgaggt gctggacttc atcgcctccg gcgacctctc ccaaggcgac gcgggcctct
      721 tccgtcccct ggtggacaac ctgctccagg acgatccgtt cctcctgctg gcggattacc
      781 aggcatatgt ggactgccag gagcgggtga gcgcgttgtg gcgcgacacg caggcgtgga
      841 cgcggatggt catcctcaac accgcccgca tgggcaagtt ctcgtccgac cggtccatcc
      901 gggattactg cgaacgggtg tggcacgtga agccgatgcc ggtggaggcg agctaacagg
      961 ctgctcaaaa tggcccatct gaggattcat gaaagatgcg cacgaatctt ggattgctcg
     1021 cggagccgta gagtgagcat acaaggagga ctgccatgac cgtcgacatc aagcgcatcg
     1081 ccatcaacac gggtggcggg gatgccccgg ggctgaacgc agtgatccac gcgaccgtct
     1141 atgctgcgcg ggcggtgggg tgggagacct tcggaatccg ggaggggtat gacggcctcc
     1201 tggatcccca gaattatccg gagggcgggc tcgtccagct cacgcccgac atggtgcgac
     1261 acattgccca tctcggcggt accatcctgg gcacgacgaa ccgagggaac cccttcaagc
     1321 acgtcgtcac gcaacccgat ggcacgacgc acgagctgaa ccgctccacc gcggtcttgg
     1381 aggcattccg taagaaccgg ctggacgcgc tcatcgcggt gggtggcgac ggatcgctcg
     1441 ccatcgcgaa ccagttgtat gaacagggtc tgcgggtggt gggcgtccac aagaccatcg
     1501 acaacgatct ggacgccacg ctggtcacgt tcggcct
//
LOCUS       DBNGZC010000853         1852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_171870, whole genome shotgun sequence.
ACCESSION   DBNGZC010000853 DBNGZC010000000
VERSION     DBNGZC010000853.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_171870"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1480..>1852)
                     /locus_tag="AC16G0_14710"
     CDS             complement(1480..>1852)
                     /locus_tag="AC16G0_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HIR5100903.1"
                     /translation="GQLAFYGSDAAAINELMEREPDLAAPVAEGLPYVGAEVVWAVRH
                     EMARTVEDVLCRRTRALFLNTKAARAAAPRVASLMAQELGYDADWKARELVAFEQVAS
                     HYSLEVDASGGIGIDPGSGRA"
BASE COUNT          352 a          639 c          533 g          328 t
ORIGIN      
        1 tcggctccag gcggcatccc cccgagcgcc gcggcgaagg cggcgcgcag cagggcggtg
       61 ccccacgcca ggggcgcgcg gcgacgaaac cacgaacggc cccgccggtc attttcatga
      121 tcagcgagcc cgccacgtga gcccccgcga gcccgcgtaa ccaccaacgt gcccccgtcg
      181 cggcctggac ggttcaggcg agacaacttg accgaacata ggggggacgg acggtatgtc
      241 aaggctgcat ggctgattca ctccggatgg tttcggcgag aatttccaca ccgccagtcc
      301 gggaaaaaag aggactgcca atgcggagtc catttcctcg gcaccctcaa cgcatgacct
      361 ctgctgcatc ggcctaaacc acctgttacg atcctccaag cgtgagcccg ccccatgtcc
      421 cggcatgggg acgccccccg gacccaaccc tcacgaggca atacctgtgg cagatgctga
      481 gcctggaagt ccagaaaatg tggccccggg gaggctaggc cgctcggagg atgcccttaa
      541 tgtccgaagg ccatcgaacc cccgctcacc ctaacgagta gtgtccccag cagcggagca
      601 cttgccttac aagcggagcg gcgaaagatc gcgattcgtc ccaggccttc ccaatcaagc
      661 agttagttag attaccggtt ccctccgttg cccgttttca cccggcattt ccctaataaa
      721 ccgtcagtag ggcgtccgta agatcaggaa ccgtatcgac accagggcgg cccatcaatg
      781 ccgcgcgata gaaaggccca cgacggatcg cgttgcttat cgggggattc tttttctgga
      841 tgggtgtgat cagcaaagac tgaactcgct ggcgatgttg gccgcatttg gactcgcggt
      901 gggcgagcca agcggttacg cgccgtagtt agccccacat tgcatgggtg cccgtgcagt
      961 ccggttttgc aaatggcccg aagctccccg cccggtcgaa ctcaccctcg cagtgcccgg
     1021 cagaggtcca tccacctccc tgatccgctg gcctttggcg agcggcgtcc ggcgagtggc
     1081 ggcttcccag ctgcgcaacg ttcgtcttga gggcatggcc cttggactcg agccccaacc
     1141 tctcggtcac gccgtcgacc gcacgattct gatcctcggg tgtcggcatc tgccctcggt
     1201 ttctgacgca agagcgggcc aacaacgtcg gaagcggcga aaccacaggc aagagcaaat
     1261 tcctggtagt cctggcgtcg cctccttgcc agcggctccc ctctcattcc gcccgacgag
     1321 ccaacttctg ttgtcatccc caaaacagga tgttccccat cagcatgctg cggagttgcc
     1381 cctcgccgtg cggagcccga tcgaggagga gtacggctgc ggcggacgag accacgtgga
     1441 ccaggctgac acaacccgcc cgagaggacc catatgccct tatgcccgtc ctgagccagg
     1501 atcaattccg atccctcccg acgcgtctac ctcgaggctg tagtggctgg caacctgctc
     1561 aaaagcgacg agttctctcg ccttccaatc ggcatcgtac cccagttcct gcgccatcag
     1621 cgatgccacg cgcggagccg ccgccctggc agccttggta tttaggaaca aggcgcgtgt
     1681 gcgccggcac agcacgtctt ccaccgtccg cgccatttca tggcgcaccg cccacacgac
     1741 ctcggctccc acatacggca gaccttcggc aacgggtgcc gccaggtcgg gctctctctc
     1801 catcaattcg ttgatggctg ccgcgtcaga gccatagaac gcgagctgtc cg
//
LOCUS       DBNGZC010000854         1148 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_757552, whole genome shotgun sequence.
ACCESSION   DBNGZC010000854 DBNGZC010000000
VERSION     DBNGZC010000854.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1148)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_757552"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..938)
                     /locus_tag="AC16G0_14715"
     CDS             complement(180..938)
                     /locus_tag="AC16G0_14715"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013402459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5100904.1"
                     /translation="MRFQDKVVLITGAGVGIGRAAAVGFAQEGAKVAVNSLTPANGKE
                     TLRLLRNAGGEGIYIQGDVSKDADARRMVDETVKAFGRLNVLVNNAGIVIPGKADNTS
                     EQDWDRTLAVNLKGAFLVSKHAIPEMRKVGGGVIVHNASVAAIKGLKDRAAYSASKGG
                     LWALTKAMAADHIAENIRVNCVCPGTTYTPSLERRIQAFADPEQAKADFIARQPMGRL
                     GADHEIAAALLFAASDEAAFMNGATIAIDGGMTI"
BASE COUNT          199 a          394 c          342 g          213 t
ORIGIN      
        1 atcctccaaa ctgaagacac acccgcgccc cacggcgcga cgcgccacac ccagcttccc
       61 cgctgggtat acgggcggcg gcgccggagc aaggctgacc ggttctcgac ggagcccggc
      121 ggccgcaggg gtgcagtcac aggtcgccga attagtaacc gaacttccaa ctcgggacac
      181 tagatggtca tccctccatc gatggcgatc gtggcgccgt tcatgaaggc ggcctcgtcc
      241 gaagcagcga agaggagggc ggcggcgatc tcatgatcgg ccccgagccg acccatgggc
      301 tgccgtgcga tgaaatctgc cttggcctgc tcggggtcgg caaacgcctg gatgcgccgc
      361 tccagggacg gcgtgtaggt cgttcccggg cacacgcagt tcacccggat attctctgcg
      421 atgtggtcgg cagccatggc cttggtcagg gcccacaacc ctcccttgga ggccgagtaa
      481 gcggctcggt ctttgaggcc tttgatcgcg gccacagacg cattgtgcac gatcacgcct
      541 cccccgacct tccgcatctc cgggatggcg tgcttggaga ccaggaaggc gcccttcaag
      601 ttgaccgcca gggtgcgatc ccaatcctgc tccgaggtgt tgtccgcctt tccggggatg
      661 acgatcccgg cattattcac caggacgttg agccggccga atgccttgac ggtctcgtcc
      721 accatgcgcc gcgcgtcggc gtccttggac acatccccct ggatgtagat cccctcgccc
      781 cccgcattcc tcaacaggcg cagggtttcc ttcccattgg ccggcgtcag ggagttcacc
      841 gcgacctttg ccccttcctg ggcgaacccg accgcggcag ctcttccgat ccctacgcca
      901 gcccctgtga tcaagaccac tttatcctgg aatcgcatca tcggtcctcc cccaaagaag
      961 tgaaccgcga cgaggcaaca cgaacgcgca cccgaggcca gcgggcgaga agccaccccc
     1021 gggatgcttc tggaatccgt cccagggtag catgattccc ttggggattc atgggcatgt
     1081 ctgcgactct ccggttttcg tatggacacg tgcaatcggc ctttgatacc atgccgcggt
     1141 cgtggctg
//
LOCUS       DBNGZC010000855         6057 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_463668, whole genome shotgun sequence.
ACCESSION   DBNGZC010000855 DBNGZC010000000
VERSION     DBNGZC010000855.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_463668"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="AC16G0_14720"
     CDS             <1..476
                     /locus_tag="AC16G0_14720"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012633110.1"
                     /note="catalyzes the formation of
                     3-deoxy-D-arabino-hept-2-ulosonate 7 phosphate from
                     phosphoenolpyruvate and D-erythrose 4-phosphate, tyrosine
                     sensitive; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR5100905.1"
                     /translation="NAIRAAARPHSFLGLSAQARSTIVRTRGTAHCHLVLRGGDDRPN
                     YDTVSISIVERALANAGLPRIVVVDCSHANSHKDPELQPLVLSDCVHQIREGNRSIVG
                     LMVESNIEGGNQPIPSDLSTLKYGCSVTDPCVDWPTTTLMLRKACQALHDILPQR"
     gene            784..2001
                     /locus_tag="AC16G0_14725"
     CDS             784..2001
                     /locus_tag="AC16G0_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR5100906.1"
                     /translation="MPRALEGVRVVDMSHVLAAPTTSMYLADLGAEVIHIEREIGDDA
                     REFGPFVGGHDKNRSAYFISLNRNKKSLVLDLKNPKGREILRDLIRVSDVVLENFRPD
                     TMAKLGLGWEALQKLNPRIIYCSICGFGHDSLPEYASRPAYDMVAQAYSGIMSITGPE
                     GGPPCRVGSSVGDIISGLQATIGILAALQYRQRSGRGQYVDIAMVDGLFSVLENAVAR
                     YVNTGEVPGPLGGAHPSITPFQGFQGKDGQWVIAAVGNDKLWALYCELLDRLDLIDHP
                     MFKTNPLRCDNRKALIPILEKEMVKKPAQEWCDLFEKHGIPYSPINNLQQICQDPHIA
                     HRKMLVEIDQPVVGKMQICASPLKLSETPGEVYAPAPMLGQHSEEVLREVLGFAPEKI
                     AQLKAEGIIGKTC"
     gene            complement(1982..3334)
                     /locus_tag="AC16G0_14730"
     CDS             complement(1982..3334)
                     /locus_tag="AC16G0_14730"
                     /inference="COORDINATES: protein motif:HMM:NF014046.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="damage-control phosphatase ARMT1 family protein"
                     /protein_id="HIR5100907.1"
                     /translation="MTEQRVEGPFTIPAGCIVPAGLPPAIRTSEPGSFARYTFTQRVP
                     RILKDTLELNDFPTAIRLALEALHAELTGGVIRGLQEDTPDRVFWNRVAAPHLGRGWL
                     EVPWYWGEAYFYRRLLEVTRYFQPGPWQHVDPFGPKKATELVPEAAPKAVIALVRHLP
                     EDPRARFETLLHASLWGNRSDLSYEVAAHLGRPTGTRDEDQDLLVDDTAQVWEALRTD
                     QIRRLAMLADNAGTELLMDLLLADELLGQGRVDEVHLHLKPQPFFVSDAMPQDVVAGL
                     EALEVAGEAAGPLVRRLRDHLKHRRLQLFTHWHYTTSLFYFQLPEDLGDVLAGMDLVI
                     VKGDANYRRLLGDAHWPPTTPFAEVTGYFPASLVALRTLKAEIIVGLRTGEAERLNAE
                     DRSWLVNGRRGVVQANLWRFTDKVGRRECRIGGPIRETPARAGRPRRGMGVPLSRSCR
                     "
     gene            complement(3462..4142)
                     /locus_tag="AC16G0_14735"
     CDS             complement(3462..4142)
                     /locus_tag="AC16G0_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HIR5100908.1"
                     /translation="MRGRFLLLWLTLWAILGTPAWGQGDPPPKARIVAGYEGIVVAVG
                     DSLTAGLGIEEDLAYPALLEKKLAAEGFRYRVINAGVSGETSSGALSRIHWILRMKPD
                     IVILETGANDGLRGIDPILTQKNIEEILRVLQGARVSVILAGMEMVQNLGQEYTTAFR
                     RIYPQAAKRFGVPLIPFFLEGVAGKPAMNQADGIHPTAAGYKVVVDTLYPYVVEAIRQ
                     RRGSRGQP"
     gene            complement(4144..>6057)
                     /locus_tag="AC16G0_14740"
     CDS             complement(4144..>6057)
                     /locus_tag="AC16G0_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5100909.1"
                     /translation="GLDLVKPGSRVDYSVLLAIADETALDETASWLRSRSLQEQERVE
                     TFRSAESGVKRFLDNFLFFLGLIGVFTLLLAGIGIDSVLTALLREQERTVGIMKAMGA
                     TSKFIIVQYLSIVSTLGMAGTLLGIGLSFGLQGILPELFKGFLPPNVEFAISWSNIFE
                     GLALGTLVVALFAFLPLYRLRDVKPHSILGKEEVRARRGIPYVLVVSTVLLLFTGIIL
                     WRLRDLRLSFFFILGAILLVLVTALATEVALFLVRKWRVRSLVVRQAMRGLFRPRNAT
                     KPIIITLAASLALLFSIYLVEQNLDASFVRSYPPDAPNLFFLDIQPAQRAEFAKVLDI
                     PTEYYPVVRGRVASINGRPVDREKERRRRGDNLGREFNLTYRNHLLEDEHIIQGKSLY
                     GSSGEGTNVSVLDEVLKMADISLGDVITFNIQGVPLEARVSSIRTRTRRDIAPFFYFV
                     FAPDTLKDAPQTIFAAVRVERQRIPALQTAIVTKFPNVSVIDATETITVFAGVMHRLS
                     SIVRFFTLFSVLAGLLLIISSVFATRFARIQEAVYFKVLGARRRFVLEVFTLENLIIG
                     LISAALALLISQTVSWLISIRFFDIQYRVFPEASLVLAALTTLLVISVGVLASLSILR
                     QKPVAFLREQADE"
BASE COUNT         1172 a         1958 c         1865 g         1062 t
ORIGIN      
        1 tcaatgccat ccgcgcggct gcccgacccc acagtttcct cggcctgagc gcccaggcgc
       61 gctcaaccat cgtacggacg cgagggaccg cccattgtca cctggtcctt cggggcggcg
      121 atgaccgacc gaactacgac acggtgagca tttccatcgt ggaacgggcg ctggccaacg
      181 cgggtctccc gcggattgtc gtcgtggatt gttcccacgc caattcccac aaggatccgg
      241 agctgcagcc cctggtgctg tccgattgtg tgcaccagat ccgggaaggc aaccgctcca
      301 tcgtcggcct gatggtcgag agcaacatcg aggggggcaa tcagccgatt ccgtctgatc
      361 tgtccacgct caagtacggg tgctccgtga cggacccgtg cgtggattgg ccgaccacga
      421 ccctcatgct gcgcaaggcc tgccaggccc tccacgacat cctcccccag cgctgaaccc
      481 cctgagcggg tctgctgcct ccgtaatctc ccaccgatct tccaccgcgc gctgatgtcc
      541 acgagcctct cgaggcgtca gagagcggaa gctggtatac cgggtgcacg cccgagagga
      601 caaatggcgg atcggcattg cgttacccga tcggggcacc tatgatgcgg catcagatcg
      661 atccgcgggg atcgccgtga aacccgcgca ccggccccgc cacgacctga ttattttcag
      721 gcggcgcccg gcgactttca cccggagtgc gatcattcaa tccgtgcctc ggaggaaacc
      781 accatgccaa gagccttgga aggcgtacgc gtcgttgaca tgagccacgt gctggcggct
      841 ccgaccacca gcatgtacct ggccgacctc ggcgccgagg tgatccacat cgagcgggag
      901 atcggcgatg acgcccgcga gtttggccca ttcgtcggcg gccacgacaa gaaccgcagc
      961 gcctatttca tcagcttgaa ccgcaacaag aagagcctcg tcctcgacct gaagaatccc
     1021 aaggggcggg agatcctgcg ggatttgatc cgcgtgtccg acgtggtgct ggagaatttc
     1081 cggcccgaca ccatggcgaa gctgggccta ggctgggaag ccctgcagaa gctcaacccg
     1141 cgaatcatct actgcagcat ctgcggcttc ggccacgact ccttgcccga gtatgccagc
     1201 cgtcctgcct atgacatggt ggcgcaggcc tacagcggaa tcatgagcat caccggtccc
     1261 gaggggggac ccccgtgccg ggtcggttcc tccgtcgggg acatcatctc cggattgcag
     1321 gcaaccatcg ggatcctggc ggcgctccag taccgccagc gctccggtcg aggccagtat
     1381 gtggatatcg ccatggtgga tgggctcttc tccgtcctcg agaacgccgt ggcgcgctac
     1441 gtgaatactg gagaagtccc ggggcccttg ggtggcgccc atccgtccat cacccccttc
     1501 cagggattcc aggggaagga tgggcagtgg gtcatcgctg ccgttgggaa cgacaagctg
     1561 tgggctctgt actgcgagct tctggatcgg ttggatctta tcgatcaccc catgttcaag
     1621 acgaatcccc tgcgttgcga caaccgcaag gccctcatcc ccatcctcga gaaggagatg
     1681 gtcaagaagc cggcccagga atggtgcgac ctgtttgaga agcacgggat cccctactcg
     1741 cccataaata atctccagca gatctgccag gatccgcata tcgctcaccg gaagatgctg
     1801 gtggagatcg accagccggt ggtgggcaag atgcagatct gtgcctcgcc gctgaagctg
     1861 tccgagacac ccggggaggt ctatgcgccc gcgcccatgc ttggtcagca ctctgaagag
     1921 gtgctgcggg aagtcctggg cttcgcgccg gagaagatcg cccagctcaa ggccgaaggc
     1981 atcatcggca agacctgctg agcggcaccc ccatcccccg gcgcggacgc ccagcccgcg
     2041 ccggggtttc tcgaataggc cccccgattc tacactccct tcttcccact ttatccgtga
     2101 agcgccagag gttcgcctgc acgacgccgc gtcgaccgtt caccagccag gatcggtcct
     2161 ccgcgttgag ccgctccgcc tccccggttc gcaggccaac gataatctcc gccttgaggg
     2221 tccgcagggc caccagcgaa gcggggaaat acccggtgac ctctgcgaac ggggtcgtgg
     2281 gcggccagtg ggcgtcaccc agcaagcgcc ggtagttggc gtcccccttc acgatgacga
     2341 ggtccatgcc ggccaggaca tcccccaaat cctcggggag ctgaaagtag aacagactcg
     2401 tcgtgtaatg ccaatgggtg aagagctgca ggcgtctgtg tttcaagtgg tcacggaggc
     2461 gtcggacaag gggccccgcc gcttcgccag ccacctccag cgcctcgaga cccgcaacca
     2521 cgtcctgggg catggcgtca gagacgaaga acggctgggg cttcaggtgg aggtgcacct
     2581 cgtccacccg cccctgaccg agcaactcat ccgccaggag aaggtccatg agcaattcgg
     2641 tgcccgcgtt gtcggcgagc atcgcgaggc ggcgaatctg gtctgtgcgc aaggcctccc
     2701 acacctgcgc cgtgtcatcc accaggagat cctggtcctc atcgcgggtg ccggtggggc
     2761 ggcccaggtg cgccgccacc tcgtaactga ggtcgctccg attaccccac aggctggcat
     2821 gcagcaacgt ctcgaagcgg gctcggggat cttcggggag atgccgaacg agtgcgatca
     2881 cggctttcgg ggcggcctcg ggaaccagct cggtggcctt cttcggcccg aagggatcca
     2941 catgctgcca ggggccaggc tggaaatagc gtgtgacctc cagcaggcgt cggtagaagt
     3001 aggcctcccc ccaataccac ggcacctcca accagcctcg gccgaggtgg ggcgccgcca
     3061 ccctgttcca gaagacccgg tccggcgtgt cctcctgcaa tccccggatc acgcctccgg
     3121 tcagctcggc gtgcagggct tccagcgcca gacgaatggc agtcggaaag tcgttcagct
     3181 cgagcgtgtc cttcaggatg cgtggcaccc gctgggtgaa ggtgtagcgg gcgaaggatc
     3241 caggctcaga ggtgcggatg gcaggcggca acccggccgg tacgatgcag ccggctggaa
     3301 tcgtgaatgg accctcgacc ctctgttccg tcacctgagc cacccattgc cccgcaagaa
     3361 ccccatccgc tcgacgccct ccccatcctc gaacgtggtg gtctgatcct tgccgaccag
     3421 gcttcactgc tggccggcaa tgagcctgtg gccaggccgg ttcatggctg acctcggctc
     3481 cccctccgct ggcggatggc ctccaccaca tacgggtaga gggtatccac cacaaccttg
     3541 tatcccgctg ccgtggggtg aatgccatcg gcctggttca ttgcgggctt cccggcgacc
     3601 ccttccagga agaatgggat gagggggacg ccgaacctct tggccgcctg gggatatatc
     3661 ctccggaagg cagtcgtgta ctcttgaccg agattctgca ccatctccat ccccgcaagg
     3721 atgacgctga cgcgtgcccc ctgcagcacc cgcaggatct cttcaatgtt cttctgggtc
     3781 aggatcggat caatgccacg caacccatca ttcgcccccg tttcgaggat gacgatgtcg
     3841 ggcttcatcc ggagtatcca gtggatcctc gacaacgccc cgctgctcgt ctccccgctg
     3901 acgcccgcat tgatcacccg gtaccggaaa ccctctgcag ccagtttctt ctccagcagg
     3961 gcggggtagg cgaggtcctc ctcgatgccg agacccgccg tgaggctatc ccctacggcg
     4021 acaacgatgc cctcgtaacc ggcgacaatc cgggccttgg gcggaggatc tccctgcccc
     4081 caagcgggcg tgcccaggat cgcccaaagt gtcagccaca gcaggaggaa tcttcctcgc
     4141 atgctactcg tccgcctgtt cccttagaaa ggcgacaggc ttctggcgaa ggatggacag
     4201 ggaggccaag acgcccacgc tgatgaccag cagcgtggtg agtgccgcca agacaaggct
     4261 ggcctccggg aaaacgcgat actggatgtc gaagaagcga atgctgatga gccagcttac
     4321 ggtctgggag atgaggaggg ccagcgcggc actgatgaga ccgatgatga gattctccag
     4381 ggtgaagacc tccaggacga agcgtctccg cgccccgagg actttgaagt acaccgcttc
     4441 ctggatccgg gcgaagcggg tagcgaacac ggagctgatg atgagcagca gccctgcaag
     4501 aacgctgaag agggtgaaga agcgcacaat ggaggacagc cggtgcatca ccccggcgaa
     4561 gacggtgatg gtctcggtgg cgtcgatgac gctcacgttg ggaaacttgg tgacgatggc
     4621 tgtttgcaat gcggggatcc gctgcctttc gacccgcacg gccgcgaaga tggtctgggg
     4681 cgcgtccttc aacgtgtctg gggcgaacac aaagtagaag aagggtgcga tgtctctccg
     4741 cgttctcgtc cggatgctgc tcacccgcgc ctccagcggc accccctgaa tgttgaaggt
     4801 gatcacgtct cccagggaga tgtcggccat cttgagcacc tcgtccagga ccgagacgtt
     4861 tgtcccttcc ccgctcgacc cgtagaggct cttcccttgg atgatgtgct cgtcttccag
     4921 gagatgattg cggtaggtga ggttgaactc ccgccccagg ttgtcccccc ttcgccgccg
     4981 ctccttttct cggtccaccg gtcgcccatt gatggaagcc accctccccc gcaccaccgg
     5041 gtagtactcc gtcggaatat ccagcacctt ggcgaactcg gctctttgcg ccggttggat
     5101 gtccaggaag aagagattcg gcgcatccgg cggatacgag cggacaaagc tggcgtcgag
     5161 gttctgctcg acgaggtaga tggagaagag aagggccagc gacgctgcca gggtgatgat
     5221 gatgggcttt gtggcattcc tcggcctgaa cagcccacgc atggcttgcc gaacgaccaa
     5281 ggaacgcacc cgccacttcc tcaccagaaa gagggcgact tcggtggcca gggcggtgac
     5341 gaggaccagg agaatcgcac ccaggataaa gaaaaagctc agcctgagat ctctgagtcg
     5401 ccacaggatg atccccgtga ataggagcaa gacggttgag accacgagca cataggggat
     5461 cccccgcctc gcccggacct cctccttgcc caaaatggag tgcggcttca catcccgcag
     5521 gcggtacagg ggaaggaagg cgaacagggc cacaaccagc gtacccagag ccagcccttc
     5581 gaatatgttg ctccaggaga tggcgaactc cacgttcggt ggtaggaacc ccttgaagag
     5641 ttccggcagg atgccctgga gtccgaagct gagaccgatc cccaggagcg tgccggccat
     5701 accgagggtg gagacgatgg acaagtactg gacgatgatg aacttactgg tcgcccccat
     5761 ggccttcatg atgcccacgg tccgctcctg ctcccggaga agggcggtca ggacgctatc
     5821 gatcccgatg cccgccagca ggagggtgaa gacaccgatg aggccgagga agaagaggaa
     5881 attatccagg aaccgcttta caccggattc ggccgatctg aaggtctcca cccgttcctg
     5941 ctcttgcagg gacctggatc tcagccagga cgcagtctcg tcaagggccg tctcgtccgc
     6001 gatggcgagg agaactgagt aatccacccg gctcccaggt ttcaccagat ccaggcc
//
LOCUS       DBNGZC010000856         1063 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_757640, whole genome shotgun sequence.
ACCESSION   DBNGZC010000856 DBNGZC010000000
VERSION     DBNGZC010000856.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_757640"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..>1063
                     /gene="tyrS"
                     /locus_tag="AC16G0_14745"
     CDS             114..>1063
                     /gene="tyrS"
                     /locus_tag="AC16G0_14745"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR5100910.1"
                     /translation="MDVEREAARQLEILKARTVDLLVAEELHRKLCRSLTAGAPLRIK
                     LGLDPTAPDLHLGHTVVIHKLREFQALGHQVVFLIGDFTAMIGDPTGRSETRKPLSTE
                     QIQANAETYKRQIFKILDPDKTVVDYNSRWGKGLGSEGIIRLAGQYTVARMLERDDFH
                     KRFTEGRPISIHEFLYPLLQGYDSVALQADVELGGTDQKFNLLVGRELMRDSGMEPQV
                     IMTLPILEGLDGVQKMSKSLGNYIGIDERPDVIFGKTMSIPDSLIVRYMELATSIPSV
                     EIDRFRGALAEQAMNPRDAKIRLAKALVAQYHTPTEAEAAA"
BASE COUNT          211 a          314 c          366 g          172 t
ORIGIN      
        1 caaggagggt catggctctc cgctgttcgg caggatgccg ggcggttttc cgttgcgagg
       61 gcagggggcc tatggtagaa ggagcgcctg ctggggacgg gagaccgggg atcatggacg
      121 tcgaacgcga ggccgcgcgg caactcgaga tcctgaaggc gagaacggtg gacctcctgg
      181 tggcggagga gctgcaccga aagctgtgcc gctccctcac ggcgggggct cccctgagaa
      241 tcaagctcgg cctggaccct accgcgcccg atctgcacct ggggcacacg gtggtcatcc
      301 acaaactccg cgagtttcag gcgctgggac accaggtggt tttcctgatc ggggatttca
      361 ccgccatgat cggggatcca acgggtcggt ccgaaacccg aaagcccctg agcacggaac
      421 agatccaggc caacgccgag acctacaagc gtcagatctt caagatcctg gatccggaca
      481 aaacggtcgt ggactacaac agccggtggg gaaaggggct gggctcggag ggcatcattc
      541 gtctcgcggg ccagtacacc gttgcccgga tgctggagcg cgacgacttc cacaagcgct
      601 tcacggaggg gcggcccatc agcatccacg agttcttgta tccgctgctc cagggctacg
      661 actcggtcgc actccaagcg gacgtggagc tgggcgggac cgatcagaag ttcaacttgc
      721 tggtggggcg cgagctgatg cgggatagcg gtatggagcc tcaggtgata atgacgctgc
      781 cgatcctcga ggggctcgac ggcgtccaga agatgagcaa gagcctgggg aactacatcg
      841 gcatcgacga gcgcccggat gtcatcttcg gcaagacgat gtcgatcccc gatagcctca
      901 tcgtgcggta tatggaattg gcgacctcga tcccctcggt ggagatcgac cgcttccggg
      961 gcgcgctggc ggagcaggcg atgaaccccc gcgacgcaaa gatccgcctg gccaaggcct
     1021 tggtcgctca gtatcacacg ccgacggagg cggaggcagc ggc
//
LOCUS       DBNGZC010000857         5202 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2028227, whole genome shotgun sequence.
ACCESSION   DBNGZC010000857 DBNGZC010000000
VERSION     DBNGZC010000857.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2028227"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(342..1370)
                     /locus_tag="AC16G0_14750"
     CDS             complement(342..1370)
                     /locus_tag="AC16G0_14750"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR5100911.1"
                     /translation="MKSDLAAPLASLAVIGAGAWGTTLAAVLAERYRQVSLWAFEADL
                     ALEMVRTRENAVYLPGVRLPDAVCPTSALDMAVSGQLLVIFAVPSHAFRGVLEKASAH
                     LAPGALVVSATKGIEVQTLSTMSQIMREVLPQPHHPKLAVLSGPSFAREVAVHRPTAI
                     VTAAFDPDVARSVQRAVNGDSLRVYAATDPLGVELGGAVKNVIAIAAGVVDGLALGLN
                     ARAALITRGLAEMTRLGVTMGAEARTFAGLAGMGDLILTCTGDLSRNRRVGLELARGL
                     KLEDIISASRTVAEGINTCRSTVGLAERHGVEMPICRAVHRVLYEGQDPREAVIQLMA
                     RDLRFEGE"
     gene            complement(1367..1780)
                     /locus_tag="AC16G0_14755"
     CDS             complement(1367..1780)
                     /locus_tag="AC16G0_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100912.1"
                     /translation="MEPKEPDNSTGNIREWLEGLRGNDLTDSDVEAAAERLLAAGGSV
                     LPILMEQFKTEDETLLAVTTLTLKRWEHPDPVKPLLDLLRNPEVSDLAKALILNVLEH
                     RGLDVDDPAVFGLAINLEEYLPEPGRGGNGKMQEP"
     gene            complement(1987..2205)
                     /gene="uxx1"
                     /locus_tag="AC16G0_14760"
     CDS             complement(1987..2205)
                     /gene="uxx1"
                     /locus_tag="AC16G0_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_354666707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(2167..2169),aa:Sec)
                     /transl_table=11
                     /product="UXX-star selenoprotein family 1"
                     /protein_id="HIR5100913.1"
                     /translation="MADTVLIFGKDTUPHTTAAREDYGKRKIPFRYLNVTKDPAELKR
                     MLQYSKGDRTVPVIVEGSKVTIGFDGGG"
     gene            complement(2236..2661)
                     /locus_tag="AC16G0_14765"
     CDS             complement(2236..2661)
                     /locus_tag="AC16G0_14765"
                     /inference="COORDINATES: protein motif:HMM:NF014058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0072669 - tRNA-splicing ligase complex
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HIR5100914.1"
                     /translation="MGKGNGFEIFPLTADKGIRAWGRDLAGLFVNAARGLWSLMVEPG
                     AVRRERMIPVKVEATDRETLLVAWLNEMLYLYETREFVMADCSILTLTDTALSAETWG
                     ESIDRERHPMLGHVKAVTYHLLQVRTTPEGWQGQVVVDV"
     gene            complement(2708..3940)
                     /locus_tag="AC16G0_14770"
     CDS             complement(2708..3940)
                     /locus_tag="AC16G0_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5100915.1"
                     /translation="MMLLATLRIALRALRTNKLRAALTMLGIIIGVGAVITMVAVGSG
                     AASRIEEQIASIGSNLLMVLPGAATSGGMRMGFGSTMTLSEDDAKAIGVEIPGVVTAS
                     GSMRGVAQIVYGNMNWSTAIQGTMPDYLVIRDWELEGGRFFTPEDVDGATKVAVLGQT
                     VKESLFGDGEALGQIIRIKKVPFTVIGTLTRKGQSTWGQDQDDVVLIPLSTAKKRVLG
                     VSQANARMVGVIQIKVASADQLTDVQQQVMDLLRQRHHLQPYQDNDFDVRNLAETFAA
                     QAEASRTMTLLLGAIASVSLLVGGIGIMNIMLVSVTERTREIGLRMAVGARSRDILTQ
                     FLIEAVTLALIGGILGILLGVGGSQLIAYLASWSTLITPTAILGAFGSAAMIGIFFGY
                     YPARKAAYLDPIEALRYE"
     gene            complement(3950..4687)
                     /locus_tag="AC16G0_14775"
     CDS             complement(3950..4687)
                     /locus_tag="AC16G0_14775"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020740289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5100916.1"
                     /translation="MVLIEVEGLTKIYQTGEVAVEALRGVDLAISAGEFVAIMGPSGS
                     GKSTFMHILGCLDTPTQGSYRLEGQLVSGMTRDQRAKVRNKKIGFVFQGFNLLSRTSA
                     VENVELPLLYNGGTAKERHALALKRLHQVGLQGREGHHPNQLSGGQQQRVAIARALIN
                     DPPILLADEPTGNLDSRTSIEIMAIFQRLNRENGITVILVTHEPDVAAYASRAIHVQD
                     GRIVQDEVVAAPNDAAEQLAALVPAGD"
     gene            complement(4735..>5202)
                     /locus_tag="AC16G0_14780"
     CDS             complement(4735..>5202)
                     /locus_tag="AC16G0_14780"
                     /inference="COORDINATES: protein motif:HMM:NF052838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permuted SH3 domain-containing protein"
                     /protein_id="HIR5100917.1"
                     /translation="VVTYDVVVAVGNPDLKLKPGMTANVRILVARHDGVLTIPNAAFR
                     VRVDTPAATGTRTGGSSGQAGTPGAARTATGAGGRGLGLGAGASADGGKQRVWVLQDG
                     RPVERRIQTGLSDGEKTEVVDGLKPGELIIIGLASGQKDNGTQRPGGPPRVRL"
BASE COUNT          919 a         1799 c         1543 g          941 t
ORIGIN      
        1 cggcccccaa agtagccccc gaccccccca cttcccatga tggcgaatcg cacaatcgtc
       61 tccacttctc ctgactcctc atgcgccttt ggtatagcac cctgaagccg agcccttcaa
      121 gcaaaaagcc cgcctcgaga acagccaggg ccgcctcgac tgccgcgctg caatccatcg
      181 ctccgatgaa aacaaccgcg gccgcctgtg cgttttctgg aaggcggccg ccctggaatg
      241 ctgatcggtc caacaggctg gtgaagagtc cgcttgcacc aaatattatt ctcggcccag
      301 ttcctggcgt caataatttg acaattccct ctggcctttc ttcactcccc ctcaaaccgc
      361 aagtctcggg ccatcaactg gatcaccgcc tcacgcggat cctgaccttc atacaggacg
      421 cgatgcaccg cccggcagat cggcatctcg accccatgcc gctccgccag gccgaccgtg
      481 gatcggcagg tgttgatccc ttccgccacg gtcctcgagg cggaaataat gtcctcgagc
      541 ttcagacccc ttgccagctc caaccccacg cgccggttcc gggagaggtc gcccgtgcaa
      601 gtgaggatca ggtcgcccat gccagcaagg ccggcaaagg tccgcgcctc cgcccccatg
      661 gtcaccccca gccgcgtcat ctcggccaaa cccctggtga tgagggccgc ccgcgcattc
      721 agcccgagtg ccagcccgtc caccacgccg gctgcgatgg caatgacatt cttgaccgcg
      781 ccccccaact ccacccccag ggggtcggtt gcggcataca cgcgaaggct gtctccattc
      841 accgcacgct gaaccgatcg ggccacgtcc ggatcgaagg ctgcggtcac aatcgccgtc
      901 ggccggtgta ccgcaacctc ccgggcgaag ctaggccccg acagcaccgc cagcttcggg
      961 tggtggggct gcggcagcac ctcgcgcatg atctgggaca tggtggagag cgtctggact
     1021 tcaatcccct tcgtcgcgct gacgaccagc gcccccggcg cgagatgggc gctggccttc
     1081 tccagcacgc cccgaaaggc atgcgagggc acggcgaata tcaccaacag ttgccctgag
     1141 accgccatgt cgagagcgga tgtcgggcac acggcatcgg gaaggcggac acctggaaga
     1201 tacacggcgt tttctcgcgt gcggaccatc tcgagggcca ggtccgcctc gaaggcccag
     1261 aggctcacct gtcggtagcg ctccgccagc accgcggcca gggtcgtccc ccaggcccct
     1321 gccccgatca ccgccaggga cgcgagtgga gccgcaagat cgctcttcat ggttcctgca
     1381 tcttcccgtt cccacctcgg ccgggctccg ggaggtactc ttccagattg atggccagcc
     1441 caaacaccgc cgggtcatct acgtccagcc cgcggtgttc cagaacattc aggatcaaag
     1501 cctttgccag atcgctgacc tccgggttcc tgagaaggtc cagcagaggc ttgaccggat
     1561 ccggatgctc ccagcgcttg agcgtcaatg tggtcacggc caggagcgtc tcgtcctcgg
     1621 tcttgaactg ctccatcaga ataggcagca ccgatcctcc ggcggccagg agacgctctg
     1681 cggccgcctc tacgtcgcta tcggtcaagt cgttcccacg caacccctcc agccactcgc
     1741 gaatattccc cgtcgaattg tctggctcct tgggctccac gtctcacctc ggcaatcttg
     1801 gttttggttc gtcctggtta cctgaacaac ccgcggaagg aacaagaggc gcttgaagag
     1861 gaagtcggtc gaatggcgcc gagcgaggga ccgggatgtg gtggcaggag agggtacccg
     1921 gcaatccgcc atctcatcgc atgagatccc gctgaggtcc gcctccggcg gaccgcttca
     1981 gacggatcag ccacccccat cgaacccgat cgtgaccttg ctgccctcca cgatcaccgg
     2041 gacggtgcga tcccctttgg aatactgaag catgcgcttc agctccgccg gatctttggt
     2101 cacgttgagg taccggaacg ggatcttgcg tttgccgtaa tcctcccggg cggcagtcgt
     2161 gtggggtcag gtgtcttttc cgaagatcag gactgtgtcg gccatctgcg catgctcctc
     2221 ttctcgactc cccaattaca catccacgac cacctgcccc tgccatccct ccggggtggt
     2281 cctcacctgg agcaggtgat aggtcacggc cttcacgtgt cccagcatcg ggtgacgctc
     2341 ccgatcgatc gattcgcccc atgtctctgc gctgagggcc gtatccgtca gggtcaggat
     2401 cgagcagtcc gccatgacga actcacgcgt ctcgtacagg taaagcatct cattcaacca
     2461 tgccaccagc agggtctcgc gatcggtggc ttcgaccttt accggtatca ttcgctcgcg
     2521 gcggaccgca ccgggctcca ccataaggct ccaaagaccg cgagctgcat tcacgaacaa
     2581 accggcgaga tcgcgtcccc aggcccggat ccccttgtcc gccgtgaggg gaaagatttc
     2641 gaagccgttc cccttaccca tggatgcatg agtcatatac catgcctagg atgcgctgga
     2701 aagaccccta ttcgtaccgg agcgcctcga tggggtcgag gtaggcggcc ttccgtgcgg
     2761 ggtagtaacc gaagaagatg ccgatcatgg cggcggaacc gaaggccccg agaatcgccg
     2821 tgggcgtgat cagcgtggac caactcgcca ggtaggcgat gagctgggag ccgccgaccc
     2881 ccaggaggat ccccaggatg ccgccgatga gcgccaaggt caccgcctcg atgaggaact
     2941 gggtgaggat gtcacgactc cgcgccccca cagccatgcg gagaccgatc tcccgcgtgc
     3001 gctccgtgac ggagaccagc atgatgttca tgatgccgat gccgcccacg agaagggaga
     3061 ccgaggcgat ggcccccagg agcagcgtca tggtccggct ggcctccgcc tgggcggcga
     3121 aggtctcggc caggttgcgc acgtcgaagt cgttatcctg gtacggctgg aggtggtgcc
     3181 gctgtcgcag cagatccatg acttgttgct gcacgtcggt gagctggtcg gccgaggcca
     3241 ccttgatctg gatgacaccc accatgcggg cgttcgcctg gctcacgccc aagacccgct
     3301 ttttcgccgt gctgagcgga atcaagacca cgtcatcctg gtcctgcccc caggtggact
     3361 gccctttccg ggtgagggtg ccgatgaccg tgaagggaac cttcttgatg cggatgatct
     3421 ggccgagggc ctccccatcg ccgaagaggc tctccttgac ggtctgaccc aggacggcca
     3481 ccttggtggc cccgtccaca tcttccggcg tgaaaaagcg accgccttcc agctcccagt
     3541 cccggatcac gaggtagtcc ggcatggtcc cttggatcgc cgtggaccag ttcatgttcc
     3601 cgtagacgat ctgggccacg ccccgcatgg agccgctggc ggtcacgacc ccaggaatct
     3661 cgactccgat ggccttggcg tcgtcctcgc tgagcgtcat ggtggaacca aatcccatcc
     3721 gcatcccgcc gctggtggcg gcaccgggga gaaccatcag caggttggag ccgatactcg
     3781 cgatctgttc ctcgatccgc gaggcggccc cgcttcccac cgccaccatg gtgatgaccg
     3841 cgcccacgcc gatgatgatc cctagcatgg tgagggcggc ccgcagcttg ttcgttcgaa
     3901 gagcccgcag ggcaatgcga agcgtagcca gcagcatcat ggttcttctc taatctcccg
     3961 ccgggacgag cgcggccaat tgctccgccg catcgttggg ggccgcgacc acttcgtcct
     4021 gcacgatgcg gccgtcctgg acatgaatcg cgcgggaggc gtacgcggcc acgtccggct
     4081 catgcgtgac caggatcacc gtaatcccgt tctcccgatt gagtcgctgg aatatggcca
     4141 tgatctcgat gctggtcctg gagtccaggt tgccggttgg ctcatcggcc aggaggatcg
     4201 ggggatcgtt gatgagggcc cgcgcgatgg ccactcgctg ctgttgcccc cccgaaagct
     4261 ggttcggatg atggccctcc cggccctgga gccccacctg gtggaggcgc ttgagtgcca
     4321 gagcgtggcg ctccttcgcc gtgcccccat tgtacaagag gggcaactcg acgttctcca
     4381 cggcgctggt gcgcgagagg aggttgaagc cctgaaacac gaaaccgatt ttcttgttcc
     4441 gcaccttggc ccgctggtcc ctggtcatgc cgctcacgag ttgcccctcc aaacgataac
     4501 tcccctgggt aggcgtatcc aagcagccca ggatgtgcat gaaggtggac ttccccgagc
     4561 ccgagggacc catgatggcc acgaactccc ccgccgagat ggcaaggtcc acgccccgga
     4621 gggcttccac ggccacctcc ccggtctggt aaatcttggt gaggccctcc acttcgatga
     4681 gcaccatgac tcaataccgc ctcacgaatt tcgaatatgg actggccgga gccgctagag
     4741 gcggacgcgc ggtgggccgc ctggccgctg cgtgccgttg tccttctggc cgctggccag
     4801 gccgatgatg atcagctcgc ctggcttgag accgtccaca acctcggtct tctcgccgtc
     4861 gctcaaaccc gtctggatgc ggcgctccac gggacgcccg tcttggagca cccagacccg
     4921 ctgcttgccg ccgtcggccg atgccccggc accaagtcct aggccgcgac cgcctgcgcc
     4981 tgtcgccgtc cgggccgcac cgggagtgcc ggcctgtcca gaggaaccgc cagtgcgcgt
     5041 ccccgtcgcc gccggagtat ccacccggac gcgaaaggcg gcattcggga tggtgaggac
     5101 cccatcgtga cgggccacca ggatcctcac gttggccgtc atgcccggtt tcagcttcag
     5161 gtcggggtta cccacggcca ccacgacatc gtaggtcacc ac
//
LOCUS       DBNGZC010000858         3364 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2326344, whole genome shotgun sequence.
ACCESSION   DBNGZC010000858 DBNGZC010000000
VERSION     DBNGZC010000858.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2326344"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1509
                     /locus_tag="AC16G0_14785"
     CDS             <1..1509
                     /locus_tag="AC16G0_14785"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5100918.1"
                     /translation="GILNATLEESPPSLSIHEEATGATVWPMMPCYNNLVLFDPLKKQ
                     ESLSTIIGELAEKWEWQDGGKTLAFHLRKGVKWHDGQPFSSKDVKYTFDVVREAPGAP
                     AKLRVNPRKLWYENVAGIDAPDPDTVIFHLKRPQPSLLLMLASGYSPVYPAHVPPAEL
                     RTKCVGTGPFKLKEYRAGEYIDLVRNPDYMLKGRPYLDGIRSLVIRERGTRYSAIQAG
                     RQDIAYPLESSKVIAEEVKHAVPQIVLVETSNNVNDNILVNFRRPLFQDPRIRRAISL
                     AVDRKAYIQAGRQGAAIPGGVMLPKPYGVWGLPEQEQAKLPGFGDIAQDRAEAVKLLS
                     QAGYGSGKPLKLTVSTRAVARYVDIASFVIDQLKRVGIEATLEQVETGVWHPKLTRRD
                     YDMAVNLTGIGPDDPDANLYENFKCGSPRNYSDYCNHEVDRLIEEQSQTLDPAKRLRL
                     VQDLDVRLQLEGARLMLGWGKEYYLMWPYVKNLVPHQSVYNYGRMQEVWLDK"
     gene            1592..2542
                     /locus_tag="AC16G0_14790"
     CDS             1592..2542
                     /locus_tag="AC16G0_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5100919.1"
                     /translation="MRAYILSRLTIAILTLLGMSIVIFILMRIAPGDIVDIIFASAGY
                     VDPAAKAEITKELGLDQPMAVQYAHWLGEILRGDLGKSYRYDIPAWQVVGPRIPISVE
                     LAVMAMIIASLIGVPAGVISAIRQDRWLDYVMRVLSLAGLSMPSFWLGMVIILFLVWS
                     LGWIPALTYISPFQDLKAHLLQFIFPALAVGYRSSALIMRITRSSLLEVMREDYVRTA
                     YAKGQRERVVVWHHALRNALLPVITVLGIEFAFLVGGLVVTETVFNLPGVARFLVEAI
                     LWRDYPVVQNLVMFIAVVVILMNLAVDLLYGWLDPRVHHG"
     gene            2535..>3364
                     /locus_tag="AC16G0_14795"
     CDS             2535..>3364
                     /locus_tag="AC16G0_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5100920.1"
                     /translation="MGDGVKAEGTHGSRGRGRWRLLRRNPLGFAGGVLVLAMILMAVF
                     ADVMATQDPVRTDASHTLSPPTGLNWLGTDYLGRDIYSRIVHGARVSLVVGIASTLLG
                     AVLGGLIGLLSGYVGGLTDLVTQRIMDILQGLPLLVLALVMSAALGPSLTNVVIAISV
                     PIIPRAARVIRSSVLAIRETPYVEAARSLGVSHLRVAFRHILPNTIGPFMVLTTAQLG
                     SAILVEAALSFLGLGVPEPYPSWGRMLSVSAAEYAQKAPWLVLFPGVAISLAVFGTNL
                     L"
BASE COUNT          540 a         1122 c         1071 g          631 t
ORIGIN      
        1 ggcatcctga atgccacgct ggaggaatcc ccgcccagcc tgagcatcca cgaggaggcc
       61 acgggcgcaa ccgtctggcc catgatgccc tgctacaaca acctggtgct cttcgacccc
      121 ctgaagaagc aggagagcct gagcaccatc atcggcgagc tggccgagaa gtgggagtgg
      181 caggacgggg ggaagaccct ggccttccat ctccggaagg gggtcaagtg gcacgacggt
      241 cagcccttca gctccaagga tgtgaagtac accttcgacg tggtccggga ggcgcccggg
      301 gccccggcca agctccgggt caatcctcgg aagctctggt acgagaacgt cgccggtatt
      361 gacgcgccgg accccgacac ggtgatcttc cacctgaagc gtccccagcc ctcgctcctc
      421 ctgatgctgg cctcgggcta ctcgcctgtg tatcccgccc acgtcccgcc cgcggagctg
      481 cggacaaagt gtgtcggcac cggtcccttc aagctgaagg agtaccgggc gggcgagtac
      541 atcgacctgg tcaggaaccc cgactacatg ctcaaggggc gtccctatct cgacggcatc
      601 cgctccctcg tgatcaggga gcgggggacg cgatactcgg ccatccaggc cggtcggcag
      661 gacatcgcct acccgctgga gagctccaag gtcatcgccg aagaagtgaa gcacgccgtg
      721 cctcagatcg tcctggtcga gaccagcaac aacgtcaacg acaacatcct ggtcaatttc
      781 cgcagaccgc tcttccagga tccccgcatc cggcgggcca tcagcctggc cgtggatcgc
      841 aaggcctaca tccaggccgg tcgccagggt gccgccatcc cgggcggcgt gatgcttccc
      901 aagccctatg gtgtctgggg gctgccggag caggagcagg ccaagctccc gggctttggg
      961 gatatcgccc aggaccgcgc ggaggcggtg aagctcttgt cccaggcggg gtacggttct
     1021 ggcaagcccc tcaagctgac cgtctcgacg cgggcggtgg cccgctacgt ggacatcgcc
     1081 agcttcgtca tcgaccagct caagcgggtg gggatcgagg ccaccctgga gcaggtcgag
     1141 accggcgtct ggcatcccaa gctgacgcgg cgggactacg acatggccgt gaacctgacc
     1201 ggcatcggcc ccgatgatcc cgacgccaac ctctacgaga atttcaagtg cggctctccc
     1261 cgcaactact ccgactactg caatcacgag gtggaccggc tcatcgagga gcaatcccag
     1321 acactggatc ccgccaagcg gctgaggctt gtccaggact tggacgtccg gctccagctg
     1381 gagggggctc ggctgatgtt gggatggggc aaggagtact atctgatgtg gccctacgtg
     1441 aagaacctgg tgccacatca gtccgtgtac aactacggcc ggatgcagga ggtctggctg
     1501 gacaagtaac ccggtgcact gccgaccgac cgcgaccggg agagggggtt tccctctccc
     1561 ggtccaggtc gcgggctgaa ggccggtcgc catgcgggcc tatatcctgt ctcggctgac
     1621 catcgccatc ctgactctcc tggggatgtc catcgtcatc ttcatcctaa tgcggatcgc
     1681 cccgggcgac atcgtggaca tcatctttgc ctccgcgggc tacgtggatc cggcggccaa
     1741 ggcggagatc accaaggaac tgggtctgga tcagcccatg gcggtgcagt atgcccattg
     1801 gctgggagag attcttcgcg gcgacttggg aaagtcctac cgctacgaca tccccgcctg
     1861 gcaggtggtc ggcccccgca tcccaatctc cgtagagttg gccgtcatgg cgatgatcat
     1921 cgccagcctg attggcgtgc ccgccggggt gatcagcgcc atccggcagg atcgctggct
     1981 cgactatgtg atgcgcgtgc tcagcctggc cggtctgtcc atgccctctt tctggctcgg
     2041 catggtgatc atccttttcc tggtctggag cctgggctgg atccccgccc tcacctacat
     2101 ctcaccgttc caggacctca aggcgcacct cctgcagttc atcttcccgg ctctcgccgt
     2161 cggctatcgc agttcggcgc tcatcatgcg gatcacccgc tcctctctgc tggaggtgat
     2221 gcgcgaggac tacgtccgca ccgcctacgc caagggacaa cgggagcggg tggtggtctg
     2281 gcatcacgcg ctgcggaacg cccttctccc cgtgatcacg gtactcggca tcgagttcgc
     2341 gttcctcgtc gggggtctgg tggtgaccga gacggtcttc aaccttccgg gagtggcccg
     2401 cttcctggtg gaggccatcc tgtggcggga ttatccggtg gtccagaatc tggtcatgtt
     2461 catcgccgtg gtggttatcc tgatgaacct cgcggtggac ctgctctacg ggtggctgga
     2521 cccgagggtg caccatgggt gatggcgtaa aagccgaggg aacccacggc tcgcggggcc
     2581 ggggccgctg gcggctcttg cgccggaacc ccctggggtt tgccggaggg gtcctggtcc
     2641 tggcgatgat cctgatggcg gtctttgcgg acgtcatggc cacgcaggat cccgtccgca
     2701 ccgatgcctc gcacactctc agccccccca caggcctcaa ctggctgggg accgactacc
     2761 tcggccgcga catctacagc cggatcgtcc acggggcgcg agtctcgctg gtcgtgggga
     2821 tcgcttccac gctcctgggt gcagtccttg gcggactgat cgggctgctg tctggatacg
     2881 tgggcggcct caccgatctc gtgacccagc ggatcatgga catcctgcag ggattgcccc
     2941 tgctggtgct ggcgctggtc atgtctgcgg cgctggggcc gtccctcacc aacgtcgtca
     3001 tcgcgatctc ggttcccatc attccacggg cggcccgagt catccgctcc agcgtcctgg
     3061 ccatccggga gaccccgtat gtggaggccg cccgctccct tggcgtcagc catcttcgcg
     3121 tggccttccg ccacatcctg ccgaacacga tcgggccgtt catggtgctc accacggccc
     3181 agctcgggag cgccatcctg gtcgaggcgg ccctgtcctt tctggggctc ggtgttccgg
     3241 agccctatcc atcctggggg cgaatgctgt cggtctccgc ggccgagtac gcacagaagg
     3301 ccccgtggct tgtcctgttc ccgggcgttg ccatcagcct ggcggtcttc ggcacgaatc
     3361 tgct
//
LOCUS       DBNGZC010000859         2099 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1636933, whole genome shotgun sequence.
ACCESSION   DBNGZC010000859 DBNGZC010000000
VERSION     DBNGZC010000859.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1636933"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="AC16G0_14800"
     CDS             <1..299
                     /locus_tag="AC16G0_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="manganese ABC transporter ATP-binding protein"
                     /protein_id="HIR5100921.1"
                     /translation="LLLLDEPVSGVDVTTQHAIFAVLEELRSEGKTVVVATHDLNCVI
                     ERFDQVLCLNRSVIAYGPPKDVFRGDILDRTYGSHLMIVDVGDRRVAVTDEHSH"
     gene            357..1190
                     /locus_tag="AC16G0_14805"
     CDS             357..1190
                     /locus_tag="AC16G0_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR5100922.1"
                     /translation="MLEFLAAPFEFAFMQRALVGGVLVGMICAMVGTYVVLRGLAFVG
                     DALAHAAFPGVVIAYLLKGNIYLGAAIFALATALGIGLVSRRARMSYDTTIGILFAGA
                     FALGVLLMSPIRGYTVDLFSFLFGNILGISGRDLLFVGLLGGAVVLTVALFYKELLLL
                     SFDPTVAEAMGYPVQALNYLLLALIALTIVISIQAVGIILVVALLVTPSATAYLLTER
                     LFRMMLLGIFLAVLAAVVGLYISYYLNLASGAAIVLVSTCLFFVVLAGRRLTSRGWEP
                     A"
     gene            complement(1655..1810)
                     /locus_tag="AC16G0_14810"
     CDS             complement(1655..1810)
                     /locus_tag="AC16G0_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100923.1"
                     /translation="MRTHVALDHLIDLEERVGGLYTSFFRRFADLPAIATSGGKWPWR
                     STSTPGP"
BASE COUNT          322 a          712 c          634 g          431 t
ORIGIN      
        1 accttctcct cctggatgaa ccggtgagcg gggtggacgt caccacgcag cacgccatct
       61 ttgccgttct ggaagagctg cgaagcgagg gaaagaccgt tgtcgtggcc acgcatgacc
      121 tgaactgcgt gatcgaacgc ttcgaccagg tcctgtgcct gaatcggagc gtgatcgcct
      181 acggtccccc caaggacgtc ttccgcgggg atatcttgga tcggacttat ggcagtcacc
      241 tgatgatcgt tgacgtaggc gatcggcgcg tcgccgtgac cgacgaacac tcgcactgac
      301 acccatccga ttgccgtgag gcggaagaga aagcccagag gggaggtgtg atcgccatgc
      361 tggagtttct ggcggctccc tttgaatttg ccttcatgca gcgcgccctc gtgggtggcg
      421 tgctggtggg gatgatttgc gccatggtgg gaacctatgt ggtgctgcgg ggactggcct
      481 tcgtcgggga cgcgctggcc catgcggcgt ttcccggtgt cgtgatcgcg tacctgctca
      541 agggcaacat ttatctcgga gccgcgatct ttgccctggc cactgctctg gggatcggcc
      601 tcgtctcccg gcgcgcccgc atgagctacg acaccaccat cggcatctta ttcgccggcg
      661 ccttcgccct gggcgtcctg ctcatgagcc cgatccgggg atacaccgtg gacctgttca
      721 gcttcctgtt cgggaacatt ctggggatct cggggcggga cctgctattc gtcggactgc
      781 tggggggagc ggtggtgctg acggtcgccc tgttctacaa ggagctgctc ctcttgtcct
      841 tcgatcctac cgtggccgag gccatgggat acccggtcca ggcgctcaac tatcttctcc
      901 tggccctcat cgctctcaca atcgtcatct ccattcaggc tgtgggcatc atcctggtgg
      961 tagccctcct ggtgaccccg agcgccacgg cgtatttgct caccgagcgc ctcttccgca
     1021 tgatgctcct ggggatcttc ctggcagtcc tcgccgccgt cgtcgggctg tacatctcct
     1081 attacctgaa tctcgcctcc ggcgccgcca tcgtcctggt cagcacctgt ctttttttcg
     1141 tggtcctggc cggtcggcgt ctcacctccc gcggatggga gccagcctga cgcctcccga
     1201 ccggacaatt ccatccggct ctccggttcc cggacgccgg cacgcgccga tcggacccgg
     1261 ctgctcccgc gctcacccgt ccgaggtcgt gaagtcgcca cggggggaac ggatcgctgg
     1321 cgcgcctcgg ggcggaggtc ccacgcttcg cgcaggaccc gttcccctcc gaacttgcgc
     1381 accgtccgca tgagccgtcc cagatgcgcc tcgtgtgggg cgaaagccga tcggggaatg
     1441 gggggcatct ccccgccaat ggcgcgaatc gtgtcacgcc ctaggcggtc aagctcggag
     1501 cgctccaagt gcaccgccat cgccaatgcc tcgcgcagcg tcacatcccg gtccgcccgt
     1561 cgctcgcagg acgcgatggt ggtctgcaag ggtcgcaacc ggtgcaggat gtccgcgatc
     1621 tttcccttgc gatccacgag cgccttcacc atcttcaggg tcccggcgtg ctcgtgctcc
     1681 tccagggcca tttcccacca gaggttgcga tggccggcag atcggcgaac cgccggaaga
     1741 acgacgtgta gagcccgccc acccgctcct caagatcgat gaggtgatcc agcgccacgt
     1801 gggtgcgcat ctcatccccc cgagcttctg cagccaagct cgcatttttc ctgcccagtc
     1861 gccagaagat ctccaccagt cgcagactat tgcaggggaa gactccccgg gtcaagttga
     1921 aacccgccgg cccgccggcc cacccctcga cgatagactg tgtcttgccg ggagatgaag
     1981 ggcaatcatc cgtcgcacat cttgggcggc cccatgcccg aatatcccac gacccacctg
     2041 ttgttccaag agggtccgaa tccccttgac tttctggccg gggtctctgt aggcttcgc
//
LOCUS       DBNGZC010000860         6549 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_955570, whole genome shotgun sequence.
ACCESSION   DBNGZC010000860 DBNGZC010000000
VERSION     DBNGZC010000860.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6549)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_955570"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="AC16G0_14815"
     CDS             <1..319
                     /locus_tag="AC16G0_14815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100924.1"
                     /translation="ALSTRGMLLEVVEAPLTWCALIVIAAGLLAWSARARLQGVIHAF
                     TWFTAAARQSFGFEGLNWLVVSATRAVATGFRITQPGHLSWNLVGIVGGLLAVLVLLT
                     WGI"
     gene            321..1829
                     /locus_tag="AC16G0_14820"
     CDS             321..1829
                     /locus_tag="AC16G0_14820"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 5 family protein"
                     /protein_id="HIR5100925.1"
                     /translation="MSATAFFWLVALPLGASPVVYVIGRLGANRDPEERAPATVRWLT
                     LLILLATWLPFSLAAKEVAQKGRVTFSVGTIGLQLDGVSILLAASVLALGTAVALHSV
                     AYMAEEVGEEKYYAMLLAMIGVMIGLGCANDLFNLWVWFEAMAVSSYLLVAFYREQPA
                     SLEAGMKYLVQSALGSVLVLMGIALVLAQTGTLHLDKIRPAAHTSPALLAAGALFIIG
                     FGVKIAMVPMHTWLPDAHSQAPSGISAMLSGVVIEAGLVALLRALIPLVGVTPSWGTL
                     LMGFGALNMLYGNLLALKQEQVKRLLAFSSLSHVGYMLLGLGISLHVGQSAGAQGAFF
                     HLFNHGLMKGSAFLAAGAMLYALHLAAGSHAPLTIQDLSGAARRYPMAALALSVAVLA
                     LGGLPPLAGFMSKWQIFVAGFATHNVLIASLVIFAAMNSVLSLAYYAPLVNAVYRKEP
                     SLAVTRGAALPRSMVLPLTVLTVLIVVLGLWPSSLNWLTEPAGAALVAAIRG"
     gene            1843..2154
                     /locus_tag="AC16G0_14825"
     CDS             1843..2154
                     /locus_tag="AC16G0_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100926.1"
                     /translation="MEVLLYPPMAFGIYLVLVAVLAGVGGTLAVPSQASQLKTGAYAS
                     GEAGESTQAAPGYRQFFVVALFFAVLHLGMLLVGSSGLTWVTGVYLIGLILALVALIL
                     G"
     gene            2171..2641
                     /locus_tag="AC16G0_14830"
     CDS             2171..2641
                     /locus_tag="AC16G0_14830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIR5100927.1"
                     /translation="MSVQTAGVWTEALDKIKTWARVNSPWAIHYNSGSCNGCDIEIVA
                     TLMPRYDLERFGVKLQGSPRHADVLICTGPVTLQSRDRLRRIYEQMPDPKFVVAVGSC
                     ATSGGVFQGCYNVVGHIDGVIPVNAYIPGCPPRPEAIVDGVVKLLQSLQAPAKA"
     gene            2655..4319
                     /locus_tag="AC16G0_14835"
     CDS             2655..4319
                     /locus_tag="AC16G0_14835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HIR5100928.1"
                     /translation="MNTETALQQAEMALAVFAKGTAKPTANRLDVALDSGDLLKAATA
                     LRQAHWGYFAALSGVDLGKDANALEALYHFCEGDAIATLRAKLPRSGGSVPSLHSLHP
                     NAVFYERELAEMFGITVTDLPDSSHLFLPDGWPEGVYPLLKDVDPHPAAQSVKGVDPP
                     ESGRRGNRFVVPIGPQHPALKEPGHFEFTVDGELVTEAKVRLGYVHRGIEKATEERNY
                     VQNLYLVERVCGICSHSHANAYALGVEQLAGVQVPPRAQAIREVVACLERVHSHLLWL
                     GVASHEAGFDTLFMYSWRDREVVMDLLEELTGNRVNYSASVLGGVKFDISPAQADTIR
                     RGLDTIDERTRHYVAVATTDESLLRRTRGIGTFTKAEAEFLEVVGPVARASDVARDVR
                     TDSPYGGYKQFPVKIVVDTAGDLAARFKVRLEEIFVSTNTIRTILDHLPEGNLTTKMP
                     RRIPPGEAISRFEAPRGELFYYIRSNGTDKPDRVKIRTPSICNWISVLQKAVGSQLAD
                     VPPLLAGIDPCFSCNDRSVVINRPRRDRQVWTWEDLRQYANKRYRR"
     gene            4324..5238
                     /locus_tag="AC16G0_14840"
     CDS             4324..5238
                     /locus_tag="AC16G0_14840"
                     /inference="COORDINATES: protein motif:HMM:NF012373.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HIR5100929.1"
                     /translation="MRALTNLLFFPSGLFLIVLALAYEFVDRKLIARFQNRVGPRWFQ
                     TVADIVKLLIKEEITPATAHPILFRALPIAALSGVLTAGLYVPLLGLTPALSFRGDLI
                     VTLYLLSLLTMCIGMAGWNSTSRFTMLGATRALTQLFSYEAPFMLALLGPAIVAGSWQ
                     ISSIVAANQGYWTLLTQPFGFVVALIGLMGKLEMPPFDAPKAKSEIVAGALTEYSGRG
                     LGLFHLGKVIELVVGLTLIAALYLGGIANPLEFFGKTLVLLVILSSLHCVLTRLRIDQ
                     TVGLWWRYGAVVVLLQWLALIAWKGIVA"
     gene            5235..5669
                     /locus_tag="AC16G0_14845"
     CDS             5235..5669
                     /locus_tag="AC16G0_14845"
                     /inference="COORDINATES: protein motif:HMM:NF024634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR5100930.1"
                     /translation="MTLGMMLGDIWTSFFRKPVTQLYPFQRLEAPAHFRGKLVWTPGK
                     CTGCGLCTKDCPSNALELITIDKKAKQFVMRYHADRCTYCGQCVKNCRLECLGMSHDQ
                     WENAGFKRSDFLVHYGNEADVEHILATFGQPSAEPAVGCADD"
     gene            5602..6165
                     /locus_tag="AC16G0_14850"
     CDS             5602..6165
                     /locus_tag="AC16G0_14850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HIR5100931.1"
                     /translation="MSTSWRLSASQALSRLSVAPTTDGGSDHPRPPRVVLVGIGQELR
                     GDDAAGVKVARSLLRRQRSRQKVDPAPETPALLILEGGPAPENCTGPIRRFQPDLVIL
                     VDSAHMGEPAGTIRWLDWKDTTGLSACTHSLPPYMFARYLTDECHCAVALIGIQPVHT
                     TLGESPSSPVGRAIRRVADSLSVLLFP"
BASE COUNT         1037 a         2160 c         2121 g         1231 t
ORIGIN      
        1 cgccctctcc acaaggggca tgctgctgga ggtcgtggag gctcccctca cctggtgcgc
       61 cctgatcgtg atcgcggcgg gcctcctggc ctggtcggcg cgcgcccggc tgcagggggt
      121 tatccacgcc ttcacctggt tcacggcagc cgcccgccag agtttcggct tcgaagggct
      181 gaactggctg gtggtttccg cgacccgcgc cgttgccacg ggcttccgga tcacccagcc
      241 gggccacctg agctggaatc tggtgggtat cgtcgggggc ctgctggccg tcctggtcct
      301 cttgacatgg ggcatatgag atgagtgcga cagctttctt ctggctggtg gcgctgccgc
      361 tcggcgcgtc gcccgtcgtg tacgtaatcg gccgtctcgg ggccaaccgg gatcccgagg
      421 aacgcgcccc ggcgaccgtc cggtggctga cgctcctgat cctgctcgcc acctggctcc
      481 ccttcagcct ggccgcgaaa gaggtggcgc agaaggggcg ggtcaccttc agcgtcggga
      541 ccatcggcct ccagttggac ggggtgagca tcctcctggc cgccagcgta ctggccctgg
      601 gaaccgcggt ggccctgcac tccgtcgcct acatggccga ggaggtcggc gaggagaaat
      661 actacgccat gctcctcgcc atgatcggcg tgatgatcgg cctgggctgc gccaacgacc
      721 tgttcaatct gtgggtatgg ttcgaggcca tggccgtctc ctcctacctt ctggtcgcct
      781 tctaccgcga gcagcccgcc tccctggaag cgggtatgaa gtatctggtg cagagcgccc
      841 tggggtcggt cctggtcctg atgggcatcg ccctggtgct cgcccagacg gggacgctcc
      901 accttgacaa gatccggcct gccgcacata cctctcctgc ccttctggcc gcgggggccc
      961 tcttcatcat cggcttcggc gtgaagatcg ccatggtgcc catgcacacc tggctgcccg
     1021 acgcccattc gcaggccccc agcgggatca gcgccatgct gtcgggcgtg gtgatcgagg
     1081 ccggcctggt ggccctgctg cgggccctga ttcccctggt gggcgtgacc ccctcctggg
     1141 gaacgcttct catgggcttc ggcgccttga acatgctcta cgggaacctc ctggccctga
     1201 agcaggagca ggtgaagcgg ctcctggcct tctccagctt gagccacgtc gggtatatgc
     1261 tcctgggcct ggggatcagc cttcacgtgg gccagagcgc aggcgcccag ggtgccttct
     1321 ttcacctgtt caaccacggt ctgatgaagg ggtcggcctt cttggccgcc ggggcgatgc
     1381 tttatgccct gcacctggcg gccggaagcc atgcccctct gaccatccag gatctgagcg
     1441 gggccgccag gcgttatccg atggctgccc tggccttgag cgtggccgtc ctggctctgg
     1501 ggggcctccc accgctggcg gggttcatgt ccaagtggca gatcttcgtg gcgggctttg
     1561 cgacgcacaa tgtcctgatc gccagcctcg tgatctttgc ggcgatgaac agcgtcttgt
     1621 cgctggcata ttacgcaccc ttggtgaacg cggtctatcg caaggagccc tctctcgccg
     1681 tgacgcgagg ggcggctctc cccaggagca tggtgctgcc tctgaccgtt ctgacagtgc
     1741 tcatcgtcgt cctcgggctc tggcccagct cgctgaattg gctcacggag ccagcgggcg
     1801 ccgcgctcgt cgcggcgatc cgcggatagg aaaggagaga ggatggaggt cctgctctat
     1861 ccgccgatgg ccttcggaat ctatctcgtg ctggtggcag tcctggctgg cgtaggcggg
     1921 acactggccg tgccgagtca ggccagtcag ctaaagaccg gcgcctacgc cagcggcgaa
     1981 gccggggaga gcacccaggc ggcgcccggg taccggcagt tcttcgtcgt ggcgctattc
     2041 ttcgccgtgc tgcatctggg catgttgctg gtgggcagca gcggcctcac ctgggtgacg
     2101 ggtgtctatc tgatcggcct catcctggcc ctggtggcct tgatcctggg ctgaggggga
     2161 acaacctgcg atgagcgtgc aaactgccgg cgtctggacg gaagccctgg acaagatcaa
     2221 gacctgggcc cgcgtgaact cgccgtgggc catccactac aacagcggct cgtgcaatgg
     2281 ctgcgacatc gagatcgtgg ccacccttat gccgcgctac gatctggagc gatttggcgt
     2341 gaagctccag gggagccccc ggcacgccga cgtcctgatc tgcacgggac ccgtgaccct
     2401 gcagtcgcgc gatcgcctcc gccggatcta cgagcagatg cccgatccga agttcgtggt
     2461 ggcggtagga tcgtgcgcca cctcgggagg cgtgtttcaa ggctgctaca acgtcgtggg
     2521 ccacatcgac ggggtcatcc ccgtcaacgc ctacattccc ggctgtccgc ctcgacccga
     2581 ggcgatcgtg gacggggtcg tgaagctctt gcagagtctc caggctccgg ccaaggcgtg
     2641 acgtgggagt caagatgaat acagagacgg cactgcagca agcggagatg gcgctggccg
     2701 tctttgccaa gggcacggcc aaaccgaccg ccaacaggct ggatgtggcg ctggacagcg
     2761 gcgatctgct caaggccgcg acggccctgc gccaggcaca ttggggatac ttcgccgccc
     2821 tcagcggggt cgatctcggg aaggatgcca atgccctgga ggccctctac catttctgcg
     2881 agggggacgc cattgccacc ctgcgcgcca agctgccgcg gagcggcggg tcggtgccca
     2941 gcctccacag cctgcacccg aacgccgtgt tctacgagcg ggagttggcg gagatgttcg
     3001 gtatcaccgt gaccgacctc ccggatagct cgcacctttt cctgcccgac ggctggcccg
     3061 aaggcgtcta tcccctgttg aaggatgtcg accctcaccc ggcggcccag agcgtgaagg
     3121 gcgtggaccc tcccgagtcg gggcggcgcg ggaatcgctt cgtcgtgccc atcgggccgc
     3181 agcaccccgc gctcaaggag ccgggccact tcgagttcac cgtggacggc gagctggtca
     3241 ccgaggccaa ggtccgcttg ggctatgtcc accgtggcat cgaaaaggcc accgaagagc
     3301 ggaactacgt gcagaacctc tacttggtcg agcgggtgtg cggcatctgc tcgcacagcc
     3361 acgccaatgc ctacgccttg ggtgtggagc agctggccgg tgttcaggtg ccgccacggg
     3421 cccaggccat ccgcgaggtt gtggcgtgcc tggagcgggt ccacagccac ctcttgtggc
     3481 tgggcgtggc ctcccatgag gcgggcttcg acaccctctt catgtatagc tggcgggacc
     3541 gagaggtcgt gatggacctc ctggaggagc tcaccggtaa ccgggtcaac tactccgcca
     3601 gcgttttggg cggtgtgaag ttcgacatca gcccggccca ggcagacacc atccggcgcg
     3661 gcctggacac catcgacgag cggacgcggc actacgtcgc cgtggccacc accgacgagt
     3721 cactcctacg ccggacgcgc ggcatcggca cctttaccaa ggccgaggcg gaattcctgg
     3781 aggtcgtcgg ccccgtggcc cgtgcctccg acgtggcccg ggatgtccgt accgactctc
     3841 cctatggtgg ttacaagcag ttcccggtca agattgtggt ggataccgcc ggggacctgg
     3901 ccgcgcgctt caaggtgcgg ctggaagaga tcttcgtgag caccaacacc atccggacaa
     3961 tcctggacca cctgccggag ggcaacctaa ccacgaagat gccgcggcga atcccgccgg
     4021 gcgaggccat cagccggttc gaggcgccgc gcggggagct gttttactat atccgaagca
     4081 acggcacgga caaaccggac cgcgtgaaga tccgcacccc gagcatctgc aactggatct
     4141 cggtgctgca gaaggccgtc ggatcccagt tggccgatgt tccccccctg ctcgcgggca
     4201 tcgatccctg cttctcgtgc aacgatcgga gtgtcgtgat caatcgaccg cgtcgcgatc
     4261 gccaggtgtg gacctgggag gatctgcggc aatacgccaa caagcgttac cggcggtgag
     4321 actatgcgcg ccctgacgaa tctcctgttc tttcccagtg ggctattcct gatcgttctg
     4381 gccctggcct acgagtttgt cgatcggaag ctgatcgcac ggttccagaa ccgggtcggc
     4441 ccgcgctggt tccagaccgt ggcggatatc gtgaagctcc tgatcaagga ggagatcacg
     4501 ccggcaaccg cccatcccat cctcttccgc gcgcttccga tcgcggcctt gagcggggtt
     4561 ctgacggcgg gcctctacgt gcccctcctg ggcctcacgc cggcccttag cttcaggggg
     4621 gatctgatcg ttacgctgta cctcctctcc ctccttacca tgtgcatcgg gatggccggc
     4681 tggaacagca ccagccgctt caccatgctg ggggcgacgc gggcgctgac gcagctcttc
     4741 tcctacgagg ccccgttcat gctggccctc ctgggcccgg ccatcgtcgc ggggagctgg
     4801 cagatcagta gcatcgtggc cgccaaccag ggttactgga ccctgctcac ccagcccttc
     4861 gggttcgtgg tggccctgat cggcctcatg gggaagctgg agatgcctcc cttcgatgcc
     4921 cccaaggcca agtccgagat cgtggccggc gccttgacgg aatacagcgg ccgtggcctc
     4981 gggctcttcc atctgggcaa ggtgatcgag ctggtggtgg ggctcactct gatcgccgcc
     5041 ttgtacctgg ggggcatcgc caatccgctg gagttcttcg gcaagaccct ggtgctgctg
     5101 gtgatcctgt ccagcctgca ctgcgtgctc accaggctcc gcatcgatca gaccgtcggg
     5161 ctgtggtggc ggtacggggc tgtggtcgtc ctcctgcaat ggctggcctt gatcgcctgg
     5221 aaggggatag tggcatgaca ctcggcatga tgttgggtga catctggacc tcgttcttcc
     5281 ggaagccggt gacccagctc tacccgttcc agcggctgga ggccccggcg catttccggg
     5341 gaaagctcgt ctggacgccc gggaagtgca ccggctgcgg cctgtgcacc aaggactgcc
     5401 cgtccaacgc gctcgagctc atcaccatcg acaagaaggc caagcagttt gtgatgcgct
     5461 atcacgccga ccgctgcacc tactgcggcc agtgcgtgaa aaactgccgc ctggaatgtc
     5521 tggggatgtc tcacgaccag tgggagaacg ccggcttcaa gcggtcggat ttcttggtgc
     5581 actatggcaa cgaggcggat gttgagcaca tcctggcgac tttcggccag ccaagcgctg
     5641 agccggctgt cggttgcgcc gacgactgac gggggatcgg atcatccccg acccccccgc
     5701 gtcgtcctgg tcggcatcgg ccaggagttg cggggcgacg acgccgccgg cgtaaaagtc
     5761 gctcgcagcc tgctccgccg acagcgctcg cgccagaaag tcgatcccgc gccagagacg
     5821 ccggccctcc ttattctcga gggcgggcct gccccggaaa attgcacggg gcctatccgc
     5881 cgcttccagc cggacctcgt catcctggtg gattcggccc acatggggga accggccggt
     5941 accatccgct ggctcgactg gaaggacacc accggcctga gtgcttgcac ccacagcctg
     6001 cccccctaca tgtttgcccg gtacctcacg gacgaatgcc actgtgccgt ggcgttgatc
     6061 ggcatccagc cggtccacac cacgctgggc gaatccccct cgagccccgt cggtcgggct
     6121 atccgccggg tggcggactc gctctcggtc ttgctgtttc cctgatcgct ccatcgaaaa
     6181 aacgcgctcc cccacgatca acgaaagatt tctggtacgt cttctccttt accttccagc
     6241 acttacctac cccacggcca tggtgagaga tttctaatct cgtcttaatc cgtctcacat
     6301 gttcaaggac tatccttctg ggcaattgga gctgtctttg gcgtgaccac aagcgacaaa
     6361 cccaagggag gtacgaacat gtggcagaca ggaaagcagt gggtgacagc acggaaagga
     6421 gtactggccg tggccgtggc cgcactcctc gtcgggagtg gtttgggaat cggattggcg
     6481 ggcggaaagc cccaatcgag aacggctagc gccgccccca tggcgtctat cgcgatcccc
     6541 tcgggggcg
//
LOCUS       DBNGZC010000861         4864 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1344608, whole genome shotgun sequence.
ACCESSION   DBNGZC010000861 DBNGZC010000000
VERSION     DBNGZC010000861.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1344608"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..783)
                     /gene="sdhB"
                     /locus_tag="AC16G0_14855"
     CDS             complement(34..783)
                     /gene="sdhB"
                     /locus_tag="AC16G0_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017549054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HIR5100932.1"
                     /translation="MGTQMIELKIKRQDGPDAPSRWEEFRIPYKPKMNVISTLMEIQR
                     NPTTVDGKKTTPPVWESNCLEEVCGACSMIINGKVRQACSALIDQLTQPITLEPMTKF
                     PLVRDLMVDRKRMFDALKRVHAWIPLDGTYNLGPGPRVDPEVGSVAYNLSRCMTCGCC
                     LEVCPQISLTSSFIGAAAISQVRLFYMNPTGRMHARERLEALMGEGGLAECGKAQNCV
                     HACPKEIPLTESIAAMGGQTTRHMLRRLLGA"
     gene            complement(840..2618)
                     /gene="sdhA"
                     /locus_tag="AC16G0_14860"
     CDS             complement(840..2618)
                     /gene="sdhA"
                     /locus_tag="AC16G0_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017551099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HIR5100933.1"
                     /translation="MAARPKTIVVGGGLAGLMAVIKIAEAGVPCQLFSFVPVKRSHSV
                     CAQGGINASVNTKGEGDSPQIHFYETVKGGDFLANQPLAKGMCEAAPGIVYLLDRMGV
                     PFNRTPEGLLDFRGFGGTLYSRTAFAGATTGQQLLYALDEQVRKYEAEGLVEKFEFWE
                     FLSVVKDEEGICRGITAQNLRTMDLQVFPADAVLLATGGCGIIFGKSTNSMVCTGTAA
                     SSVYQQGAFYANGEFIQVHPTAIPAEDKVRLMSESARAEGGRMWVPKDGKPWYFLEEK
                     YPKYKNLVPRDIATREIYYVCRVLKLGVDGTDTVYLDLSHIPKETLDRKLGGILEIYE
                     KFVGDDPRKVPMKVFPGVHYSMGGLWIDYEADTSGNPVVGSPRNHQTNVPGLFAAGEV
                     DYEYHGANRLGANSLLSCFYGGMLAGPAMVSYIKAQEKSCEAVSPAVFEAERRRQEQA
                     YAAVLGLHGTENPFVIHKELGEVMTENVTVVRHNDKLRAADGALQVFMDRWKRIDIND
                     SAKWANQAVSFTRQLWNMLELARVITLGALQRDESRGAHYKPDFPERNDAQWLKTTLA
                     SWTPNGPEIRYAPVDISLLKPELRKY"
     gene            complement(2630..3256)
                     /locus_tag="AC16G0_14865"
     CDS             complement(2630..3256)
                     /locus_tag="AC16G0_14865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     transmembrane subunit"
                     /protein_id="HIR5100934.1"
                     /translation="MATSSMGQYYLRRLHSLSGIFPIGVFLLEHFFGNAFATRGPEAY
                     NRYVEFLLGMPYLPALEIGVVFGPLLFHGIYGLFIAAEGDLVHPAWGLGARYQNAAYL
                     VQRITGILLLAYISYHVWNTRVQAVFFGQHVDYAYMVRYFSPGYEKTIYIVGILCACY
                     HFANGLFNFAYKWGITVGAKAQQAMTYVSLAVFVAMSAVGIHILFSFK"
     gene            complement(3455..3766)
                     /locus_tag="AC16G0_14870"
     CDS             complement(3455..3766)
                     /locus_tag="AC16G0_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100935.1"
                     /translation="MRAVCRSVSVGLALLIAVVVTGCGQEVKRENEQLKSQVASLQKE
                     NVDLKGQMTGLKADADALKKQVENLSKEKQDLEDKVKEAEAKALAKPGTKPPLKPKKS
                     S"
     gene            complement(3864..4799)
                     /gene="mdh"
                     /locus_tag="AC16G0_14875"
     CDS             complement(3864..4799)
                     /gene="mdh"
                     /locus_tag="AC16G0_14875"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR5100936.1"
                     /translation="MARKKIALIGAGQIGGNLAHLAAAKELGDVVLFDIPAAEGTAKG
                     KALDIAQLLPVEGYDARLVGTSSYDEVAGADVCIITAGIPRKPGMSREDLLGTNIKII
                     TDVATNLKRVCPQAFCIVVANPLDAMVYAFKQLTGLPKQRVVGMAGILDSARFRAFIA
                     MELHVSVEDVSAFVLGGHGDDMVPLTRCCSVGGIPVTELLPKDRIDAIVDRTRKGGGE
                     LVALYKTGSAYYAPAASAIAMAESYLKDKKRVLPCAAFLEGEYGIRGYYFGVPVVIGA
                     GGVEQVLEVKLSEEERALVLKSLESVKKTVAETKL"
BASE COUNT          812 a         1656 c         1502 g          894 t
ORIGIN      
        1 agccccgtgc gtgggctcgc cgcggctgag ccctcaggcg ccgagcaggc gcctcagcat
       61 gtggcgggtc gtttggccgc ccatggcggc gatggactcg gtgaggggga tctccttggg
      121 gcaggcgtgg acgcagttct gggctttgcc gcactccgcc aggccgccct cgcccattag
      181 ggcctcaagc cgctcccggg cgtgcatccg tcccgtgggg ttcatgtaga agagccgcac
      241 ctgcgagatg gccgcggcac cgatgaagct ggaggtgagg ctgatctgtg ggcagacttc
      301 caggcagcag ccgcaggtca tgcagcgcga aagattgtag gccacgctcc ccacctccgg
      361 gtccacccga ggacccggcc cgaggttgta ggtgccgtcc agggggatcc aggcgtggac
      421 gcgcttgagg gcatcgaaca tccgctttcg gtccaccatg aggtcgcgga ccagcgggaa
      481 tttcgtcatc ggctccaggg tgatgggctg ggtgagttgg tcgatgaggg cgctgcaggc
      541 ctggcgcacc ttgccgttga tgatcatgct gcaggcgccg cacacctcct ccaggcagtt
      601 cgactcccag accggaggag tggtcttctt tccgtcgacg gtggtggggt tccgttgaat
      661 ctccatcaag gtggaaatca cgttcatctt gggtttataa gggatgcgga attcctccca
      721 gcgggacggg gcgtcggggc cgtcctgccg cttgatcttc aactcgatca tctgcgttcc
      781 catctgtgtc tcgcctccca tgggacatgc cggccgcggg acaggcgcgc cggacgaagt
      841 caatacttgc gaagctcggg cttgagtagc gagatgtcca ccggcgcgta gcgaatctcc
      901 ggcccgttcg gggtccagct ggccagcgtc gtcttcaacc actgggcgtc gttccgctcc
      961 ggaaagtccg gcttgtagtg ggcgccccgg ctctcgtccc gctgcaaggc ccccagggtg
     1021 atcacccggg ccagctccag catgttccag agctgccgcg tgaaggacac ggcctggttc
     1081 gcccatttgg cgctgtcgtt gatgtcgatc cgcttccagc gatccatgaa gacctgcagg
     1141 gcgccgtccg ccgcccgcaa tttgtcgttg tgccgcacca cggtgacgtt ctccgtcatg
     1201 acctccccca gctccttgtg gatcacgaac ggattttccg tcccgtggag accgaggacc
     1261 gctgcgtagg cctgctcctg tcgccggcgc tcggcctcga aaacagcggg ggacacggcc
     1321 tcgcaggatt tttcttgcgc cttgatgtag ctcaccatgg caggccccgc cagcatcccg
     1381 ccgtagaagc aggagaggag cgagttggcc cccagccggt tggctccatg gtactcgtag
     1441 tccacctcgc ccgcggcgaa tagaccgggg acgttggtct ggtggttccg cggactgccc
     1501 accacgggat tgccggaggt gtccgcctcg taatcgatcc acaggccgcc catggagtag
     1561 tggacgcccg ggaagacctt catggggacc ttgcgcgggt cgtcgccgac gaacttctcg
     1621 tagatctcca ggatcccgcc cagcttccgg tccagcgtct ccttggggat gtggctgaga
     1681 tcgaggtaca ccgtgtccgt cccgtccacc cccagcttca gcacgcggca gacatagtag
     1741 atctcgcgcg tggcaatatc ccgcgggacc agattcttgt acttggggta cttctcttcc
     1801 aggaagtacc agggcttgcc gtccttgggg acccacatcc ggcccccctc ggcccgcgcc
     1861 gactcgctca tcaggcggac cttgtcctcg gcgggaatgg ccgtggggtg gacctggatg
     1921 aactccccgt tggcgtagaa ggccccctgc tgatagacgg acgaggccgc cgtgccggtg
     1981 cagaccatgg aattggtgct cttgccgaag atgatcccgc agccgcccgt ggccaagagc
     2041 accgcatccg ccgggaaaac ctggaggtcc atggttcgga ggttctgcgc cgtgatgccc
     2101 cggcagatcc cctcctcgtc cttcaccacc gagaggaact cccagaactc gaacttctcc
     2161 accagcccct ccgcctcgta cttgcgcacc tgctcgtcca aggcgtacag gagttgctgc
     2221 cccgtggtcg ccccggcaaa cgccgtccgg ctgtagagcg tgcctccgaa gccccggaag
     2281 tccagcagac cctcgggcgt ccggttgaac ggcacgccca tgcgatccag gaggtagacg
     2341 atcccgggcg ctgcctcgca catccccttg gccaggggct ggttggccag gaagtctccc
     2401 cccttgacgg tctcgtagaa gtggatctgg gggctgtccc cttccccctt ggtgttcacg
     2461 gaggcgttga tcccgccctg agcgcagacg gaatgtgatc gcttcaccgg cacgaaggag
     2521 aacaactggc acggcacccc ggcctcggcg atcttgatca ccgccatcag gccggccagg
     2581 ccgccgccca caacgatggt cttgggtctc gcagccatga cccctccccc tacttgaagg
     2641 aaaagagaat gtggatgccc acggcgctca tggcaacgaa gaccgccagg ctgacatagg
     2701 tcatggcctg ctgcgccttg gcgcccacgg tgatgcccca cttgtaagcg aagttgaaga
     2761 gcccattggc gaagtggtaa cacgcgcaca ggatgccaac gatgtagatc gtcttctcat
     2821 agcccggcga gaagtagcgg accatgtagg cgtaatcgac gtgctgcccg aagaagacgg
     2881 cctgcacccg cgtgttccag acgtgatagc tgatgtaggc cagcaggagg attcccgtga
     2941 tccgctgcac cagataggcg gcattctggt agcgagcccc cagcccccag gcggggtgga
     3001 ccagatcccc ctcggccgcg atgaagagcc cgtaaatccc gtggaatagc aatgggccga
     3061 agaccacgcc gatctccagc gcgggcagat acggcatgcc cagcaggaac tccacatagc
     3121 gattgtaggc ctcgggcccg cgggtggcga aggcgttgcc gaagaaatgc tccagcagga
     3181 agactccgat gggaaagatg ccactcaacg agtgcaaccg gcgaaggtaa tactgcccca
     3241 tgcttgacgt ggccatgctc attcctctcc tcagtgtcgg cgcctccagg gccgggcgga
     3301 aacggcccag cgcgtgccgt gtcgggcggc cccctcttcg atcctcacgc ctccccaccg
     3361 atccggggtg cagggcgcca gcggaccaag ggcccgtccc tccgctcccc cgcgaggctc
     3421 gcggcgtcga aagaacgggc cccgacagtg gctgtcacga ggacttcttc ggcttcagcg
     3481 gcggcttggt tcccggcttg gccaaggcct tggcctccgc ctccttgacc ttatcctcta
     3541 ggtcctgctt ctccttgctc agattctcga cctgtttctt cagggcatcc gcatccgcct
     3601 tgaggccggt catctgcccc ttgaggtcca cgttctcttt ctgcaggctg gccacctgag
     3661 acttgagctg ctcgttctcc cgcttcacct cctggccgca ccccgtgacg accacggcta
     3721 tcagcaaggc gaggcccaca ctgacgcttc ggcataccgc acgcatccgt tcctgcctcc
     3781 tttcgaacga agagccgcaa accgacagcg atctcccaca gcccgattcc gttcgctgtc
     3841 cctgtccctg tcgctgtcgg ttcttacagc ttcgtctccg ccaccgtttt cttcacgctc
     3901 tccaggctct tcaggacgag ggcccgttcc tcctccgaca gcttcacctc cagcacctgc
     3961 tccacgcccc ccgccccgat caccaccggg acgccaaagt agtacccgcg gatcccgtac
     4021 tccccttcca ggaaggcggc gcagggcaga acccgcttct tgtccttgag ataggattcg
     4081 gccatggcga tggccgaggc cgcgggggcg taataagcgc tgccggtctt gtagagggcg
     4141 accagctccc cgccgccctt tcgcgtgcga tccacgatgg cgtcgatccg atccttgggg
     4201 agcagctccg tgacagggat cccccccacg ctgcaacagc gggtgagggg caccatgtca
     4261 tccccatggc cgcccaggac aaaggccgag acgtcctcca ccgagacgtg gagctccatg
     4321 gcaatgaagg cgcgaaaccg cgccgagtcg aggatccccg ccatccccac cacgcgctgc
     4381 ttggggagcc cggtgagctg cttgaatgca tacaccatgg cgtccagggg attggcgacc
     4441 acgatgcaga aggcctgggg gcagacgcgc ttcaggttcg tggccacgtc cgtgatgatc
     4501 ttgatgttgg tccccaggag gtcctcgcgg ctcatgcccg gcttgcgggg aatgccggca
     4561 gtgatgatgc agacatcagc cccagccacc tcgtcgtagg aactggtgcc gacgaggcgc
     4621 gcatcatatc cctctaccgg gaggagctgg gcgatgtcca gcgccttgcc cttggcggtc
     4681 ccctcggccg cagggatatc gaagaggacg acgtcaccga gttccttggc cgccgccaga
     4741 tgggccaggt tgccgccgat ctgtcccgcg ccgatcaagg caatcttctt gcgtgccatg
     4801 gctcccctcc ctcgccccgg gcgcccctca catgtgcgcc acgagggcgg tcgcaaactc
     4861 cgag
//
LOCUS       DBNGZC010000862         3631 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2028551, whole genome shotgun sequence.
ACCESSION   DBNGZC010000862 DBNGZC010000000
VERSION     DBNGZC010000862.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2028551"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="AC16G0_14880"
     CDS             complement(<1..642)
                     /locus_tag="AC16G0_14880"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5100937.1"
                     /translation="MLWQQIANGVATGAVYALTTLGLTMVYGVLRILHVAHAGIYALG
                     AYAGLAAFRAVGSFWLSLVAAMAVCALAGLLVERCLYAPLMHLPRIIPLIASIGLFIA
                     MEEAFRILAGPYALAYDAEIRFGRLALGPLQFSAPQTLILVVTLILLGLLWMLLSRSK
                     IGLAMRATAMDPEITSTFGVNTRAVVTLNFLIGSALAGAGGVLIGVYDNQVWPT"
     gene            complement(849..2018)
                     /locus_tag="AC16G0_14885"
     CDS             complement(849..2018)
                     /locus_tag="AC16G0_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5100938.1"
                     /translation="MGQKSWKRMGWLLAVAAISTLVAMPALAQETIKIGFFAPITGPV
                     AADGASAKQSVELAVKDINAAGGIKGKKIELIVYDDRLNPQEAVAIANKLIEKDQVAG
                     VVSGSYSGPTRVTAPIFAKAGVPMVAGYAVHPDVTKAGVSNFRNGYLGEVEGGAAGEY
                     AVKTLKSKSPAVINMDNDFGREISAGFIKRAEKLGAKVTVQQVYKYPGEKDFRPFLTR
                     IKETKPDLIFAAGYYDVAALIVRQAKELGLSVPILAEEGFDSPKFIELAGKEAEGVII
                     ATNLDRDDPRPVVQNFLKNYKQAYNIDPDMVGASSYDAFMILVNAIERAGSTDKQAVV
                     KALLDTKDYNGLTGKISRFVKGEVVKPVQFQIVKDGKFQRHGLVDNPEVITPPVQ"
     gene            complement(2192..>3631)
                     /gene="hutH"
                     /locus_tag="AC16G0_14890"
     CDS             complement(2192..>3631)
                     /gene="hutH"
                     /locus_tag="AC16G0_14890"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HIR5100939.1"
                     /translation="AARPKIEAARHVIESALAAERPIYGVSTGFGPLSDVFVGAADRE
                     ALQRNLLRSHAMGVGDPLGEAETRATVLLRANVLAKGHSGVRPEIVTLLCELLNRGVH
                     PVIPERGSVGASGDLAPLAHLALVLIGEGEARYRGERLPGAEALQRAGLTPITPAAKE
                     GLALINGTCGMTAIGALALIRAQRMVRLADVSSAMTLEALRGSQVPFDPRLQAVRPHP
                     GQATTAKNLLLLLRDSEVMVSHKDCGKIQDSYTLRCIPQVHGGVRDVLAFAQGTLTRE
                     LNAATDNPLIFPESGDILSGGNFHGHPVALALDALGMALASLCSIAERRIERLVNPLL
                     SELPAFLVKSSGLHSGFMVAQIAAASLVSEAKLLASPASVDSIPTSGSKEDFVSMGWL
                     AAVKLAQIVDRLAAILALEFLCAAQGLDFLRPLKPGRGVLVAHELLRRHVPHLEQDRV
                     LRHDLDRVLPLLEDESLLAAVEAAVGPLG"
BASE COUNT          611 a         1144 c         1254 g          622 t
ORIGIN      
        1 ggtcggccat acctggttgt catagacgcc gatgaggacg ccgcccgctc ccgccagggc
       61 tgagccgatg aggaagttca gcgtcaccac cgcacgggtg ttgactccga atgtcgaggt
      121 gatctccggg tccatggccg tggcccgcat ggccagcccg atcttggatc gggagaggag
      181 catccagagg agcccaagca ggatcagcgt cacgaccagg atgagggtct ggggagcgct
      241 gaactgcaag ggaccgaggg ccaggcgtcc gaatcggatc tccgcgtcat aggccagggc
      301 gtagggaccg gccaggatgc ggaaggcttc ctccatggca atgaagaggc cgatgctggc
      361 gatcaagggg atgatgcggg gcaggtgcat gagcggcgca tacaggcagc gctcgacgag
      421 gaggccggcc agggcgcaga cagccatagc cgccaccagg ctgagccaga agctgcccac
      481 ggcccggaag gccgccaggc ccgcgtaagc acccagggcg tagatgccgg cgtgcgccac
      541 gtgaagaatg cgcaggacgc cgtagaccat ggtgaggccc agggtcgtca aggcgtacac
      601 cgccccggtg gcgacgccgt ttgcgatctg ctgccagagc atttctgagg aaaccattga
      661 tccacagatt acgcagatta cgccgattat cccgagggct tcgggccgga acctttcagg
      721 gatctgcgaa atctgcgaaa tctgcggaaa aattcgggtc ttcggtggaa ccggaacaca
      781 agagggcggg gggccgaacc ggtcagcccc ccgcccttcg ccgctgtctc tgccgcattc
      841 tctctgggtt attgcaccgg cggcgtgatg acctcgggat tgtccacgag gccgtggcgc
      901 tggaacttgc cgtccttgac gatctggaac tggaccggct tcaccacctc gcccttcacg
      961 aagcggctga tcttccccgt gaggccgtta tagtccttgg tgtcgaggag ggccttgacc
     1021 acggcctgct tgtcggtcga gccggctcgc tcgatggcgt tgaccaaaat catgaaggca
     1081 tcgtagctgg aggcccccac catatccgga tcgatgttgt aagcctgctt gtagttcttg
     1141 aggaaattct gcaccacggg gcgggggtca tcgcgatcca gattcgtggc gatgatcacg
     1201 ccttccgcct ccttgccggc cagctcgata aacttgggtg agtcgaaccc ctcctcggcc
     1261 aggatcggga cggagagccc cagctccttg gcctggcgga cgatcagggc ggcaacgtcg
     1321 tagtagccgg cggcgaaaat gagatcgggc ttggtctcct tgattcgggt gaggaacggc
     1381 cggaagtcct tctccccggg gtacttgtac acttgctgga ctgtcacctt ggcgccgagc
     1441 ttttcggccc gcttgatgaa accggcggag atctcccggc cgaaatcgtt gtccatgttg
     1501 atgacggctg ggctcttgga cttcagggtc ttgacggcgt actcgcccgc cgcgccgcct
     1561 tccacctcac cgaggtatcc gttgcggaag ttggacacgc ctgccttggt aacgtccggg
     1621 tggacggcat agcccgccac catggggact ccggccttgg cgaagatcgg agccgtcact
     1681 cgcgtcgggc cgctgtagga gccgctgacc acgccggcga cctggtcctt ctcgatcagc
     1741 ttgttggcga tggcgacggc ctcctgaggg ttgaggcgat cgtcgtagac gatgagctcg
     1801 atcttcttgc ctttgatccc accagccgcg ttgatgtcct tcacggccag ctcgaccgac
     1861 tgcttcgcgc tcgcgccatc ggcagcgacg ggcccggtga tcggcgcgaa gaagccgatc
     1921 ttgatcgtct cctgcgccag ggcgggcatc gccacgagcg tcgagatcgc ggcgactgcg
     1981 agcagccatc ccatcctctt ccagctcttc tgacccatga atcattcctc ctcttgtgcc
     2041 gctggggtcg cgatttggaa cgccttgcgc cggattccgg caccacctgc cgaccgcagg
     2101 tgggaatggc ccaattaggc cacggatcgt ggcgcctcgt caaggaccca gtcgtccgtg
     2161 agtgttcacg ctgcggtttt cggatcgaag ttcagccgag cgggccgacg gccgcctcca
     2221 cggcggcaag gaggctctca tcctccagga gcggcagcac ccggtcgagg tcgtgccgga
     2281 ggacacgatc ctgctccagg tggggcacgt ggcggcgcag cagttcgtgc gccacgagaa
     2341 caccccggcc cggcttgagc gggcgcaaga agtccagccc ctgggcggcg cacaggaact
     2401 ccagggccag gatcgcggcc agacgatcca cgatctgggc gagcttgacg gccgccagcc
     2461 accccatgct gacgaagtcc tccttgcttc cggaggtggg aatggaatcc acgctggccg
     2521 gggacgccag gagcttcgcc tcggacacca gggaggcggc cgcgatctgc gccaccatga
     2581 atccggagtg gaggccgctg gacttcacca ggaaggccgg cagctcgctg agcagggggt
     2641 tcaccagtct ttcgatccgc cgctcggcga tgctgcagag gcttgccagg gccatgccca
     2701 gggcgtccag ggccagggcg accgggtggc cgtggaagtt gccccccgag aggatgtccc
     2761 ccgattccgg gaagatcagc gggttgtccg tggccgcgtt cagctcgcga gtgagcgtgc
     2821 cctgggcaaa ggccaggacg tcccggacgc cgccgtgaac ctgggggatg cagcgcaggg
     2881 tgtagctgtc ttggatcttg ccgcagtctt tgtgggagac cattacctcg gagtcccgga
     2941 ggagtagtag gaggttcttg gccgtggtcg cctggccggg gtgggggcgt actgcctgga
     3001 gccgcggatc gaaggggacc tgggatccgc gcagggcctc cagcgtcatg gcggaggaca
     3061 catcggccaa tctcaccatg cgttgggccc ggatcagggc cagggcgccg atggcggtca
     3121 tgccgcaggt accgtttatc agggccaagc cttctttggc cgcgggggtg atcggcgtga
     3181 ggccggcccg ctgcagcgct tcggcgcccg gtaggcgctc gccccgatac cgagcctcgc
     3241 cttcgccgat caggaccagg gccaggtggg ccaggggggc caggtctccg ctggctccga
     3301 cggaaccgcg ctccgggatg acggggtgca ccccgcgatt cagcagctcg caaagcaggg
     3361 tcacgatctc cggccggacg cccgagtgtc ccttggccag aacgttggca cggagcagga
     3421 cggtggcccg ggtctccgcc tcgccgaggg ggtcgccgac gcccatggcg tggctgcgga
     3481 ggagattccg ctgcaaggct tcacgatcgg cggcacccac gaagacatcg ctcagcgggc
     3541 cgaagcccgt gctgactccg tagatgggac gctcggcggc cagggcggac tcgatgacgt
     3601 ggcgggcggc ctcgatcttg ggccgggcag c
//
LOCUS       DBNGZC010000863         2868 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2714600, whole genome shotgun sequence.
ACCESSION   DBNGZC010000863 DBNGZC010000000
VERSION     DBNGZC010000863.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2714600"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="AC16G0_14895"
     CDS             <1..1110
                     /locus_tag="AC16G0_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100940.1"
                     /translation="CTVANRVFYGNVTIGGVRFPTMVAWSAAGDRTNNPALARSNLLD
                     TGDHIVAVKRMGRLAAVVYREWSQWLATAVAGSDATAFQFSVQDRQPGPIGAAAIADG
                     PGLRHVYLGSDLNLYTFDSVSSVLLAPTADVLRSRLDPTNRALVNVVYSPVDQEVWVT
                     LPLDGDSIPTHVMVYSLQSQGVFFQRLPTALAVTMLGTWQSELGTPTDLLPDIPTDTL
                     PDIPTNQLGFVAGRVVLLAGSLQSVFTLDALTDDTSDITATGEYLLPITEEDYEFDGL
                     NVMIATPGSPVTVSVKVGPVFDQLTETVLGVVDPGIAAGIDTNLAAPGPNLAELRTPG
                     PMRGRIVVIKFTTTTSQEVKIRRLEIFAFPKKRLA"
     gene            1107..1709
                     /locus_tag="AC16G0_14900"
     CDS             1107..1709
                     /locus_tag="AC16G0_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100941.1"
                     /translation="MRSGTRLVVGFVGLVGLAVTGCATLTPGPDDRAEPVTVDLSQFN
                     AEVRVKVEQMVVDAKKLRVEFDEKLYADLVVAGRIANEEPPPDPVAGICFPVMAQKVR
                     QRIDATGGIRPVGPFSFFEKANKVIFPLSVPDETLKMSCSALVQRTIIKAAQIIAQVA
                     GLVSSMGTSAAGSVAEGVDKLKKILSVLHTLGMPIPLGGA"
     gene            1714..2055
                     /locus_tag="AC16G0_14905"
     CDS             1714..2055
                     /locus_tag="AC16G0_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100942.1"
                     /translation="MSLSETFQWPPKPVFTKDPIPYLDEAYANTAKEVNRPGWRHAGQ
                     AAVTGGATAVVVTEPFDRLPIYITATPNWDTTVFIVARGTTGFTLNFGTAAPGGGGTA
                     DWLAVEPDQGP"
     gene            2052..2279
                     /locus_tag="AC16G0_14910"
     CDS             2052..2279
                     /locus_tag="AC16G0_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100943.1"
                     /translation="MSVRLELWDTDGVPWKEVESPTAPLEYVVPVLKGCHRTSLDEPP
                     PLESGHVCWEKRRFQRVAISGARTYIYREVA"
     gene            2280..2468
                     /locus_tag="AC16G0_14915"
     CDS             2280..2468
                     /locus_tag="AC16G0_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100944.1"
                     /translation="MTADEATRRIRKVMADGHPCQLVINVADGGRITSIEVHQRLAVD
                     RGEEVLLDFASPTEAFSR"
BASE COUNT          491 a          896 c          980 g          501 t
ORIGIN      
        1 tgcaccgtgg ccaaccgtgt gttctacggc aatgtcacca tcggcggcgt ccggtttccc
       61 accatggtgg cgtggagtgc ggcaggggat cggacgaaca acccggcgct ggcgcggtcg
      121 aacctgctcg atacggggga ccacattgtg gccgttaagc gcatggggcg gttggcggcc
      181 gtggtctatc gagaatggtc gcagtggttg gcgacggccg tggcggggag cgacgccacg
      241 gcctttcaat tcagcgtgca ggaccgccag cccggcccca ttggcgcggc ggccatcgcc
      301 gatgggccgg gcctgcgcca tgtctatctc ggctcagacc tgaacctcta cacgtttgat
      361 agcgtctcgt cggtgctgct cgcaccaacc gccgatgtgc tgcggtcgcg cctggacccg
      421 accaaccgcg ccctcgtgaa cgtcgtgtat tccccggtag accaggaagt gtgggtcacg
      481 ctgcccctgg atggcgacag cattcccacg cacgtcatgg tgtactccct ccagagtcag
      541 ggggtctttt tccagcgcct tcccactgcc ctggcggtca cgatgctcgg cacctggcag
      601 tcagaactgg gcacccccac cgatctgctg cccgacatcc cgacagacac gctgcccgac
      661 atcccgacca accagttggg attcgtcgcg ggccgagtgg tgctgctggc gggctctctg
      721 caatccgtgt ttacgcttga cgcgctgact gacgacacca gcgacatcac ggcgacgggc
      781 gagtatttgc tgcccatcac cgaggaagac tacgagttcg atggcctcaa cgtgatgatc
      841 gcgacccccg gcagcccggt cacggtctcg gtgaaggtcg ggccggtctt tgaccaactg
      901 accgaaacgg tgctgggggt ggtggacccc ggtatcgcgg cgggcatcga cacgaacctg
      961 gccgcgccag ggccgaatct cgcggaactg agaacgccag ggccgatgcg aggccggatc
     1021 gtggtcatca agttcaccac cacgacctcg caggaggtga agattcgtcg gctggaaatc
     1081 tttgccttcc ccaagaagcg ccttgcatga ggagcggcac gcgcttggtc gtcgggtttg
     1141 tggggctggt cggcctcgcc gtgaccggat gcgccacgct gacgcctggt cccgacgacc
     1201 gtgccgagcc ggtcaccgtg gacttgtcgc agttcaacgc ggaggtcaga gtcaaggtcg
     1261 agcagatggt cgttgacgcc aagaaactgc gcgtggagtt cgatgagaaa ctctatgccg
     1321 acctcgtcgt ggcgggccgc attgccaacg aggagccgcc cccagatccg gtcgccggga
     1381 tctgcttccc ggtcatggcc cagaaggtgc gccagcgtat cgacgcaacc ggcgggattc
     1441 gaccggtcgg acccttcagt ttttttgaga aggccaacaa ggtgatcttc ccgctgtccg
     1501 tgcccgacga gaccctgaaa atgtcctgct cggcgttggt ccagcgcacg atcatcaagg
     1561 cggcgcagat catcgcccag gtggcggggc tggtgtcgtc catggggacc tctgccgccg
     1621 gcagcgtggc cgagggggtt gacaaactga agaagatcct gtcggtcctt cacacgcttg
     1681 ggatgcccat tcccctcggg ggcgcatgag accgtgagtc tctccgagac cttccagtgg
     1741 cccccgaagc cggtcttcac caaggacccc atcccgtacc tggacgaggc atatgcgaac
     1801 acggccaagg aggtgaaccg gccggggtgg cgacacgcgg ggcaagcggc ggtcacgggc
     1861 ggggccacag cggttgtggt caccgaaccc ttcgaccggc tgcccatcta catcacggcc
     1921 accccgaact gggacaccac ggtgttcatc gtggcgcggg gcaccacggg gttcacgctc
     1981 aatttcggca cggcggctcc gggtggtgga ggcaccgccg actggctagc cgtggagccg
     2041 gatcaaggac catgagtgtc cgcctcgaac tctgggacac ggacggcgtg ccgtggaagg
     2101 aagtggagtc gccgaccgcg ccgctggaat acgtcgtccc ggtactgaag gggtgtcatc
     2161 gcacctcgct ggacgagcct ccgccgctgg agtcggggca tgtctgctgg gaaaagcgcc
     2221 gattccagcg cgtggcgatc tctggagcca gaacctacat ctaccgggag gtggcctgaa
     2281 tgaccgctga cgaggcgacc cgcagaatcc gcaaggtgat ggccgacggc cacccctgcc
     2341 aactcgtcat caacgtggcg gacggggggc ggatcacctc gattgaggtc catcagcggt
     2401 tggccgtgga ccggggtgaa gaagtcttgc ttgactttgc gtcaccgacg gaggcattct
     2461 cacggtagat tcgcggtagt gtactagcgc ccaccgcgcc agacaggtgc gaggccgagg
     2521 ttgaggggtg gtctcccttg accccggcct ttttgtttgc tcgcgggggc accggaaagg
     2581 gggtggcgac tcaatggcgc gacggctgcg gcgtggcgga cggcgggggc ggcggaagta
     2641 ggccctcggc cttgtcctga gtagtccggg gcaaccagtg ggttgatgta cacacgcgga
     2701 gtagctcccc gtcgtgccgg tagttgtggg ccggagccgg gcggtgctcc ccacttcaag
     2761 gaggaacgga ccatggcgcg gaagatgcgt cgcggcggtc gtcgccacaa gcgctactag
     2821 cccctgcggg cgggtagtca cttcgagggg ttcatgccag acggattc
//
LOCUS       DBNGZC010000864         2863 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1637343, whole genome shotgun sequence.
ACCESSION   DBNGZC010000864 DBNGZC010000000
VERSION     DBNGZC010000864.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1637343"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            446..1498
                     /gene="recA"
                     /locus_tag="AC16G0_14920"
     CDS             446..1498
                     /gene="recA"
                     /locus_tag="AC16G0_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005997956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR5100945.1"
                     /translation="MAEVATRKAPGKEERKDRDKALDLALSQIEKQYGKGAIMRMGIS
                     GALLPVSTISTGSLELDFALGVGGVPRGRVVEIFGPESSGKTTLALHIIAEAQKRGGD
                     VAFVDAEHALDAKYAKTLGVNIDDLLISQPDTGEQALEITEVLVRSGAIDVVVIDSVA
                     ALVPRAEIDGEMGDSLPGLQARLMSQALRKLTAAISKSKTCVIFINQMREKIGIMFGN
                     PETTTGGRALKFYASVRLDIRRIATLKDGEDVIGSRVKVKVVKNKVAPPFREAEFDLI
                     YGEGISRLGSLLDLGVTHKVVEKSGSWYAYNGERIGQGRENAKRFLQENPTMADDIEA
                     KLRAALGMTVQPEAEA"
     gene            1499..2644
                     /locus_tag="AC16G0_14925"
     CDS             1499..2644
                     /locus_tag="AC16G0_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR5100946.1"
                     /translation="MDLTELLKIAVARKSSDLHIKVGSPPVLRIDNRLVPLLEMPRLS
                     QDVVIAMAAGVMNAKQRDRFKDQNEVDLAYSVPGLGRFRVNVFQQRGMVGIVLRLIPI
                     KIQSIAELNLPPVLEKLAQEPRGMILVTGTTGSGKSSTLAAMIDYINSTRSGHVLTIE
                     DPIEFLHRDKKCLVNQREIGVDALSFSEALRSALRQDPDIILVGEMRDYETISTAILA
                     AETGHLVMSTLHTLDAPETVNRVISVFAPYQQKQIRLQLAAVLKGIVSQRLIPRADGK
                     GRVPAVEVCIATATVRECIMDADKTRQLHDVIAAGGSQYGMQTFDQSLMSLYTRGLIT
                     YEEALHWCTNADDFALRVRGIESTADSRWQQAMQSEGPQNIPGMTRL"
BASE COUNT          569 a          850 c          951 g          493 t
ORIGIN      
        1 gactggcatg gcatatcctc cttgttggag ttaagcgcaa aatgaattgt agcgcctcat
       61 cacaacggga gataggcaga ccgaaaaaac aaacgccctc gccggtccga aaggccgaca
      121 agggcatgaa ttcagtcccg gaatgcgata ccaaggcatc gtagagtttc tctccgagga
      181 ccgtgaggta ataagatgtc agatttcgtg ggattctaga ccaagactgg gggcggctgt
      241 caagtccaga gccgaatttc caacgttctt gctgctgttg tcagatctgg actcggatgg
      301 tggaagagcc atcctggatg tcgaactgcg tatgcaggtt cccggctggc tatcctgttg
      361 acttcacctg ggaaatcacg tacgctccgc caagcgccgt ttttcatcta actggctggg
      421 gccaaagggc cggagggaat tcatcatggc tgaggtggcg acgcgcaagg cacccggaaa
      481 agaggaacgc aaggaccgcg acaaagcgct cgatctggcg ctctcccaga tcgagaagca
      541 gtacggcaag ggcgcgatca tgcgcatggg gatctcgggc gccctgctgc cagtctccac
      601 catctccacg ggctccctgg agctggactt tgccttgggc gtgggagggg tccctcgcgg
      661 ccgggtcgtc gagatcttcg gccccgagtc cagcggcaag acaaccctgg ccctgcacat
      721 catcgccgag gcccaaaagc ggggcgggga tgtggccttt gtggacgccg agcacgctct
      781 ggatgcgaag tatgcgaaga cgctgggggt caatattgac gacctcctga tctctcagcc
      841 cgatacgggt gagcaagcgc tggagattac cgaggtcctg gtgcgaagcg gcgccatcga
      901 cgtggtggtg atcgattccg tggcggccct ggttccccga gccgagatcg acggggagat
      961 gggggactcg ctgccggggc tgcaagcccg cctcatgtcc caggccctgc gaaagcttac
     1021 cgcggccatc agcaagtcaa agacttgcgt gatcttcatc aaccagatgc gcgagaagat
     1081 cggaatcatg ttcggtaatc ctgagacgac aaccggcggc cgggcgctga agttctacgc
     1141 ttcggtcaga ctcgacatcc ggcgaatcgc caccctgaag gacggcgagg atgtcatcgg
     1201 tagccgggtc aaggtcaagg tggtgaagaa caaggtcgcg ccgccgttcc gcgaggcgga
     1261 gttcgacctg atctacgggg aggggatctc gcgtctgggg agcttgctgg atctgggtgt
     1321 gacccacaaa gttgtggaaa agagtggatc ctggtacgca tacaatggcg agcggatcgg
     1381 ccaggggcgc gaaaatgcca agcgcttcct tcaggagaat cccaccatgg ccgatgacat
     1441 cgaggccaag ctgcgggcgg cgttgggcat gacggtccag ccagaggcgg aggcgtagat
     1501 ggatctcaca gagctcctca agatcgcagt agcgcggaag tcctcggacc tccacatcaa
     1561 ggtggggagt ccgccggttc tccgcatcga caaccgactg gttcccctgc tggagatgcc
     1621 gcgcctgtcc caggacgtgg tcatcgccat ggctgccggc gtgatgaatg ccaagcagcg
     1681 ggaccgcttc aaggatcaga acgaggtgga tctggcctac agcgtgccgg ggctgggccg
     1741 tttccgggtc aacgtgttcc agcagcgggg gatggtgggg atcgtcctgc ggctgatccc
     1801 catcaagatt caatccattg ccgagctgaa ccttcccccg gtcctggaaa agctggccca
     1861 ggagccccgg ggtatgatcc tggtcacggg gaccaccgga tcgggcaagt cctcgaccct
     1921 ggcggccatg atcgattaca tcaactctac gcgatccggg cacgtcctca ccatcgagga
     1981 tcccatcgag tttctccacc gggacaagaa gtgtctggtc aaccagcgcg agatcggggt
     2041 ggatgccctc tccttcagcg aggctctgcg gagcgccttg cggcaggatc ccgacatcat
     2101 tctggtgggg gagatgcggg attacgagac catctccacc gccatcctgg ccgccgagac
     2161 gggccacctg gtgatgagca ccctgcatac gctggacgcc ccggagacgg tcaaccgggt
     2221 catctcggtg ttcgccccct accagcagaa gcagatccgg cttcaactgg cggccgtgct
     2281 caaggggatc gtctcccagc gcctgatccc ccgggccgac ggcaaggggc gcgtccccgc
     2341 ggtggaggtg tgcatcgcca ccgccaccgt gcgggagtgc atcatggatg cggacaagac
     2401 tcggcaactg cacgatgtga tcgccgccgg cggcagccag tacgggatgc agaccttcga
     2461 tcagtccctg atgtccctgt acacccgcgg tctcatcacc tacgaggagg ccctccactg
     2521 gtgtacgaac gccgacgact tcgccctccg ggtgcggggg atcgagtcca ccgccgattc
     2581 gcgctggcag caggcgatgc agagcgaggg gccccagaac attccgggga tgacacggtt
     2641 gtgagagccg cgagtcccga gccgggcacc cgcgccgtag cgcgggtggg cgcgaggcgg
     2701 cggtcgcgga acgcctgagg gagcaggatc gcgtgaaaac ccggggggga gtgcagcgag
     2761 cagagtaaac gtggatcgcc cggaggcctg ggaggttgca ctgcggctcc tggccctgct
     2821 ggcccgcagc acgcaggaag tccagcagct tctcgcgcgg cga
//
LOCUS       DBNGZC010000865         3559 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_660038, whole genome shotgun sequence.
ACCESSION   DBNGZC010000865 DBNGZC010000000
VERSION     DBNGZC010000865.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_660038"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..899)
                     /locus_tag="AC16G0_14930"
     CDS             complement(90..899)
                     /locus_tag="AC16G0_14930"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5100947.1"
                     /translation="MRSRWIRLWATGVVWMGAMLPSSVTSAATLDCLIEPFRIVNVAS
                     PLDGVLERVDVERGDLVKQGQVLALVEGSVQRAALEIAEARAQLETAVESGQVRLDYA
                     IRKVASNEKLLEEHGISEREVDDSRAQRDLSRIALVEAQDNKRLAVLDVRRAAAELEV
                     RTIRSPINGVVMERILSPMEQTKQTPIVKLAQLDPLRVEVFAPISLLGKVSVGMTAEV
                     VPEVPVGGVPTARVTVVDRVVDAASGTFGMRLELSNPGYRLPAGLKCKVRL"
     gene            complement(916..2910)
                     /locus_tag="AC16G0_14935"
     CDS             complement(916..2910)
                     /locus_tag="AC16G0_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR5100948.1"
                     /translation="MSDTPRIPDLPTGAYPERLEPRGNALDRLASRVRGVVLSRWMSR
                     QRRWQALVEAVAEQGRSVAGMDDTGIRSLSEDLRLRLRREGFQEDLVVRTFALVREAA
                     ERSLGQRHFDVQIVGGWILLQGLVAEMETGEGKTLTATLAAATAALAGIPVHVITVND
                     YLAERDAEWMGPIYRALGLSVGTIIHGMDPRARRAAYGCDITYCTNKEIVFDYLKDRL
                     ALGRTRGRIHLQVERLAGAAHAPQLLLRGLHYGIVDEADSVLVDEARTPLIISGAAHP
                     SAQAEKTIYETALVLSDNLEMGRDYQVEERERFVRLTEAGEFRLSELAQDLGGVWTGR
                     IRREELVRQALTSRHLFHRDKQYLVHDGKVQIIDEYTGRTMADRSWEHGLHQLIEAKE
                     GCPVTRENAPLARISYQRFFRRYLKLAGMTGTAREVAGELAAVYGLATVSIPTNRPLH
                     RRRLKDRVYPTAAVKWEAVVERIAGLHESGRPVLVGTRLVRTSEELSQRLTAAGLPHR
                     VLNARQDQEEAETIAKAGERENITVATNMAGRGTDIRLAPGVAELGGLHVILTERHEA
                     RRIDRQLFGRCGRQGDPGSFEAMVSLEDELVGGKKVRSLRWMAARMAGRRRLVPGWMG
                     GLVFRRAQRAAERLHSRMRWDLLKFDERQETMLAFSGRGE"
     gene            complement(2903..>3559)
                     /locus_tag="AC16G0_14940"
     CDS             complement(2903..>3559)
                     /locus_tag="AC16G0_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR5100949.1"
                     /translation="LATQVDVLSARLRELKARYDEQHPVDRVKAAMVLEETRYVEGDL
                     AKAREQLESLAIRSRVDGTFVVDSAEDLPDRFVRRGDLLAHVVTLDTITVRTIVAQTN
                     ADLVRHHTQRVEVRLSERVGETLSAAIKREVPGASERLPSAALGLGGGGTVAVDPRDT
                     HGITAMEKVFQIDLTLPSDRRILNVGGRVYVRFDHGWEPLAVQWARQLRQLFLARLNV
                     "
BASE COUNT          557 a         1301 c         1096 g          605 t
ORIGIN      
        1 gttcaccgca gccgcccgag ccacgagcat gctcgcggtc caggcggacc tcctcttccc
       61 agcgtcccca gctttccggc ccgtcaacgc tagaggcgca ccttgcactt caggcctgcc
      121 ggcagccggt acccggggtt gctcagctcc aaccgcatgc caaaggtgcc gcttgccgcg
      181 tccaccaccc ggtccacgac cgtgacgcgt gcggtgggga ccccgccgac cggaacctcg
      241 gggacaacct ccgccgtcat tcccacggac accttgccca gcaggctgat cggggcgaag
      301 acctccaccc tcagcgggtc gagctgggcc agcttgacga tgggtgtttg tttcgtctgc
      361 tccatcggcg acaggatgcg ttccatcacc acgccgttga ttgggcttcg gatagtccgg
      421 acctcgagtt ccgccgcggc tcgccgcacg tcgagcaccg ccagccgctt gttatcctgc
      481 gcctccacca aggcaatccg gctcaggtct cgctgcgccc gcgagtcatc cacctcccgt
      541 tcggatatgc cgtgctcttc caggagcttc tcgttgctcg cgaccttgcg gatggcataa
      601 tccagccgga cttgccccga ctcgaccgcg gtctccagtt gtgcgcgcgc ctccgcgatt
      661 tccagggccg cccgctgcac gctcccctcc accagggcca gcacctgtcc ctgcttcacc
      721 agatccccgc gctccacgtc gacccgctcc aacaccccgt ccagcggcga tgcaacgttg
      781 acgatccgga agggttcgat cagacaatcc agggtcgccg cagacgtcac gctagacggc
      841 agcatcgcac ccatccacac cacgcccgtt gcccacagcc ggatccatct ggatctcata
      901 ggacgcctct cctcgtcatt caccccgacc ggaaaaagcc agcatggttt cctgacgctc
      961 gtcaaacttg agcaggtccc agcgcatccg ggagtgcaga cgctccgcgg cccgttgggc
     1021 ccggcggaaa accagacctc ccatccatcc gggtacgagc cgacggcgtc cagccatcct
     1081 ggcggccatc cagcgcaagc tcctcacctt cttgcccccg accagctcgt cctcgagcga
     1141 caccatggcc tcgaagctgc cggggtctcc ctggcggccg caccgcccga agagttgtcg
     1201 atcgatccgg cgcgcctcgt gccgctccgt caagatcacg tgtaagccgc caagctcggc
     1261 cacaccgggc gccagacgga tgtccgtccc ccgccccgcc atattggtcg ccaccgtgat
     1321 gttctcacgc tccccggctt tggcgatggt ctcggcctct tcctggtcct ggcgtgcatt
     1381 cagcaccctg tgggggaggc cggccgccgt gagtcgttga ctcaactctt cggaggtccg
     1441 caccaggcgc gtcccgacaa ggaccgggcg gccgctctcg tgcagaccgg caattcgctc
     1501 gacgaccgcc tcccacttta cggcggcggt gggatacacc cgatccttca gtcggcggcg
     1561 gtggagcggc cgattggtcg ggatggagac cgtcgccagc ccatagaccg ccgccagctc
     1621 gccggccacc tcacgcgccg ttccggtcat ccccgccagc ttcaggtagc ggcggaaaaa
     1681 ccgctggtaa ctgatgcggg ccagcggcgc gttctctcgg gtcaccgggc aaccttcctt
     1741 ggcctcgatg agctggtgca gcccgtgctc ccacgaacgg tccgccatgg tgcggcctgt
     1801 gtattcgtcg atgatctgca ccttcccgtc gtggaccaga tactgcttgt cgcggtggaa
     1861 caggtgccgc gaagtcaagg cctgacgcac caattcctcg cgacggatgc ggcccgtcca
     1921 gacgcctccg agatcctgcg ccagctcgct caggcggaac tcgcccgcct cggtgaggcg
     1981 gacgaatcgc tcgcgctctt cgacctggta atcccgcccc atttcgagat tgtcgctcag
     2041 gacgagggcg gtctcgtaga tggtcttctc ggcctgggcg gagggatggg ccgctccgga
     2101 gatgatcaac ggcgtgcgcg cttcgtccac cagcacgctg tcggcttcgt ccacgatccc
     2161 gtagtggagg ccccggagca ggagctgcgg cgcgtgggcg gctcctgcca ggcgctcgac
     2221 ctggaggtgg atgcgaccgc gggtacgacc gagggcgagt cggtccttga gatagtcgaa
     2281 gacgatctcc ttgttcgtgc agtaggtgat gtcgcagccg tacgccgccc ggcgcgcccg
     2341 gggatccatc ccgtgaatga tggtccccac cgagagcccc aaggcgcggt agatggggcc
     2401 catccactcc gcatcgcgtt ccgccaggta gtcgttcacc gtgatgacgt ggacgggaat
     2461 ccccgccagg gcggcggtgg cggcggccag ggtggccgtg agggtcttcc cctcgcccgt
     2521 ctccatctcg gccaccaacc cctggagcag gatccagccg cccacgatct ggacatcgaa
     2581 gtgacgctgg cccaggctgc gctctgccgc ttcccgcacc agggcgaagg tgcggaccac
     2641 gagatcctct tgaaaaccct cccgtcgaag acggaggcga aggtcctcgg acagactccg
     2701 gattccggtg tcatccatcc cggcgaccga tcgtccttgt tcggcgacag cttccactag
     2761 ggcctgccag cggcgctggc gactcatcca gcggctcagc accacgcccc ggacgcggga
     2821 agccagacgg tcgagggcgt ttccccgcgg ttcaagccgt tcggggtagg cccccgtcgg
     2881 aaggtcagga atgcgtggcg tgtcagacat tgagccgggc caggaagagc tggcgaagct
     2941 gacgggccca ttggacggcc aggggctccc agccgtgatc gaaccggacg tacacccggc
     3001 cgccgacgtt caggatccgc cgatccgagg gcagcgtcag atcgatttgg aacaccttct
     3061 ccatggccgt gatcccatgc gtatcgcgcg gatccacggc gactgtgccg cctcccccga
     3121 ggccgagggc cgcgctgggc agccgctcgc tggcaccggg aacctcgcgc ttgatggcgg
     3181 cggacagcgt ctcacccaca cgctccgaca gccgcacctc gacccgttgc gtgtggtgcc
     3241 tcacgaggtc cgcgttcgtc tgggccacga tggtacggac agtgatcgtg tccagggtca
     3301 cgacgtgcgc gagaaggtcg ccgcgccgaa cgaaccggtc cggcagatcc tccgccgaat
     3361 ccaccacgaa ggttccatcc acgcggctcc ggatggcgag gctctcgagc tgctcgcgag
     3421 ccttggccag atccccttcc acgtaccggg tctcctccag caccatcgcc gccttgactc
     3481 ggtccaccgg gtgttgctcg tcgtagcggg ctttgagctc ccggagcctc gcgctcagca
     3541 cgtcgacctg ggtggccag
//
LOCUS       DBNGZC010000866         2286 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1145042, whole genome shotgun sequence.
ACCESSION   DBNGZC010000866 DBNGZC010000000
VERSION     DBNGZC010000866.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1145042"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="AC16G0_14945"
     CDS             complement(<1..364)
                     /locus_tag="AC16G0_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing response regulator"
                     /protein_id="HIR5100950.1"
                     /translation="MTRSILLVDQDPKQRAPLLRALDGNGVRVVGEAAKPDEAMRLAA
                     TAQPNVALVAITPGNEEAIRLVDRLDTELGIPSILLVEAADQPPFPQVTKSGVIGLLV
                     QPVDPAIVHAMLDVADYRF"
     gene            complement(448..2082)
                     /locus_tag="AC16G0_14950"
     CDS             complement(448..2082)
                     /locus_tag="AC16G0_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR5100951.1"
                     /translation="MLAAEEPTKESPAAPGVVVPPASAQATAAAPAPPKPDPGGGATG
                     GLGDITAKIAGKPTLEEVADATGHNKISINIMWTLVAGFLVMFMQAGFALAETGLTRA
                     KNAAHTMMMNFMVYGLGMTGYWIMGYALQMGGVGAVSSLSGGTANLANEFTITLFGKE
                     FGLFGMTGFFLSGVAYDAAVFALFLFQMVFMDTAATIPTGAMAERWKFSSFVIFAFFI
                     SMITYPLYANWVWGGGWLSTLGKNFGLGHGHVDYAGSSVVHMVGGVTALAGAWVLGPR
                     LGKYNRDGSPNAIPAHDIPMALLGAFILAFGWFGFNAGSSLAGTDLRIGVIATNTMLA
                     SASGAMGAMFYMWSRYGKPDPSMAMNGMLAGLVAITAPCAFVNSVLAFIIGAVAGVLV
                     CLSVFFVERRLKVDDPVGAISVHGTCGAWGVLSLGLFADGTYGDGFNGVTGTVRGLFY
                     GDASQFAAQVIGTLTNFVFIFVASWAFFKVCDMVMGLRVAPEVEIEGLDSPEMGALAY
                     PDFSLIPTHAGIGLSPEPATVMSGGAFATAKKPQTA"
     gene            complement(2187..>2286)
                     /locus_tag="AC16G0_14955"
     CDS             complement(2187..>2286)
                     /locus_tag="AC16G0_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014111501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR5100952.1"
                     /translation="ARTGRIGDGKIMVTDVLEVMRIRTGEMGEAAI"
BASE COUNT          462 a          732 c          704 g          388 t
ORIGIN      
        1 gaaacctata atctgcgaca tccagcattg catgaacgat cgcgggatca accggttgca
       61 cgaggaggcc gatgacgccc gacttggtca cctgagggaa cggcggctga tcggctgcct
      121 caaccaggag gatcgatggg atgcccaatt ccgtgtccag tcgatcaacc aggcggatgg
      181 cttcttcgtt tccaggggtg atcgccacca acgccacgtt gggctgggca gtggccgcca
      241 gccgcatggc ctcgtcgggc ttcgccgcct cgccgaccac ccgcacccca ttgccatcca
      301 gggcccgcag caggggcgct cgctgcttgg gatcctggtc gacgagcaat atggatcgcg
      361 tcacgtcctt tgctccctgg cgcggctgac aggccggccc gcggcgatga tgagagccgc
      421 cgcggaccgg tccgcgagag gaggctctta cgccgtctgg ggtttctttg cagtggcaaa
      481 ggcccccccg gacattacgg tggcgggctc cggagacaat cctatgccgg catgggtcgg
      541 aatcagactg aagtcaggat aagccaaggc gcccatctcg ggactatcca ggccctcgat
      601 ttctacctcg ggcgccacac gcagacccat gaccatgtcg cacaccttga agaaggccca
      661 agaggcgacg aagatgaata cgaagttggt cagcgtgccg atgacctgcg cggcgaactg
      721 actggcatct ccgtagaaca atccacggac ggttccggtc actccgttga agccgtcgcc
      781 gtaggttcca tctgcgaaga ggccgaggct caggacaccc caggccccgc aggtcccgtg
      841 gacggagatg gcgcccaccg gatcgtccac cttgagccgc cgctcgacga agaagacgct
      901 caggcagacg agaacaccgg ccaccgcgcc gatgatgaac gccaacacgc tgttgacgaa
      961 agcgcagggg gcagtgatgg ccaccagccc ggcgagcatc ccattcatcg ccatgcttgg
     1021 atcgggcttt ccgtacctcg accacatgta gaacatggca cccatggcac cggaagcgga
     1081 agccagcatc gtgttggtgg cgatcacgcc gatccggagg tccgtgccgg ccagggaact
     1141 gccggcattg aagccgaacc agccgaaggc cagaatgaag gccccaagga gcgccatcgg
     1201 aatgtcatgg gcgggaatgg cgttcgggct cccgtcccgg ttgtacttac caaggcgcgg
     1261 gccaaggacc catgccccgg ccaaggctgt aacgcctccc accatgtgga cgacggatga
     1321 gccggcatag tcgacatggc catgcccaag accgaagttc ttcccgaggg tgctcagcca
     1381 gccgcctccc cagacccagt ttgcgtagag cgggtaggtg atcatggaga taaagaacgc
     1441 gaagataacg aacgacgaga acttccatcg ctcggccatc gcgcccgtgg gaatcgtcgc
     1501 cgcggtgtcc atgaacacca tctggaataa gaacagcgcg aagactgccg cgtcgtaggc
     1561 gactccgctc aggaagaatc cagtcatgcc gaagagcccg aattcttttc cgaagagggt
     1621 gatggtgaac tcattggcca gatttgccgt tcctcccgac aatgaactca cggcgccgac
     1681 accacccatc tggagggcgt agcccatgat ccagtagccg gtcatgccga ggccgtacac
     1741 catgaagttc atcatcatgg tgtgggcagc gttcttggcg cgggtgaggc cggtctccgc
     1801 caaggcgaac ccagcctgca tgaacatgac cagaaagccg gccaccaggg tccacatgat
     1861 gttgatggag atcttgttgt ggccggtggc gtcagcgacc tcctccaagg ttggcttgcc
     1921 ggcaatcttg gccgtgatat cgccgaggcc accggtggcg ccaccgcccg gatccggctt
     1981 gggtggagca ggggctgcgg cggtggcttg tgccgatgct ggaggcacca cgactcctgg
     2041 agcggctggc gactccttgg tcggctcctc tgcggccagc atggagccag ccagcaccgg
     2101 gctaatccag atggcaatga cgagccccag cgagatcatc cttcggcaac gtctcatgac
     2161 ctctctcctc tccctgcgtc gggttgtcaa atcgcagctt cacccatctc gcccgtacgg
     2221 atgcgcatga cctcgagcac gtcagtcacc atgatcttcc catccccgat tcggccggtc
     2281 cgggct
//
LOCUS       DBNGZC010000867         6298 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1540694, whole genome shotgun sequence.
ACCESSION   DBNGZC010000867 DBNGZC010000000
VERSION     DBNGZC010000867.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6298)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1540694"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..338
                     /locus_tag="AC16G0_14960"
     CDS             18..338
                     /locus_tag="AC16G0_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HIR5100953.1"
                     /translation="MSTVVLAGKEMEVDEDGFIQDPALWDEEVAAALAKAEEGIDEMT
                     PDHWKLVHYLRNYYLQFQIAPMVRKLCKETGFSLQKVYDLFPSGPAKGACKVAGLPKP
                     TGCV"
     gene            362..757
                     /locus_tag="AC16G0_14965"
     CDS             362..757
                     /locus_tag="AC16G0_14965"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR5100954.1"
                     /translation="MRLEHDLAFQATVRDHRILMDMRGHPRFADAGPSPVELLVMAIG
                     GCIGMHVALFCEQMNLSPAGLRVDLSFALADQNGRKRVSSVYAEVDAPGIPTDLLPAA
                     ERAARLSILPNSFVKTPELDIEVRSGSGQ"
     gene            763..1359
                     /locus_tag="AC16G0_14970"
     CDS             763..1359
                     /locus_tag="AC16G0_14970"
                     /inference="COORDINATES: protein motif:HMM:NF025719.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsbRD N-terminal domain-containing protein"
                     /protein_id="HIR5100955.1"
                     /translation="MAIEDAAPLLDALKARKGAIVKAWIARTLHAYPDHTFRFLDQEK
                     DPFRNPVGHTLKEGLAALLEELLGGMNAEKITPLLNDIVRIRAVQDCTAAQAVAFPFL
                     LKNVIRETLHSSSCPPLSPSGGKDKGDAGSWSDALAPLEDRIDEMALLAFDLFMRCRE
                     SMYEVRVNETKRRLYLLQRMHASSLPSAGDDPGVPSKS"
     gene            1393..2385
                     /gene="dsrM"
                     /locus_tag="AC16G0_14975"
     CDS             1393..2385
                     /gene="dsrM"
                     /locus_tag="AC16G0_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrM"
                     /protein_id="HIR5100956.1"
                     /translation="MHAQQALAVVMILAALGFVGGQATSVRTLFGVVVPWSALAVFLL
                     GFIFQVIRWSRSPVPFRIPTTCGQQKSLAWIQASRLENPASTLGVLGRMVLEIGLFRS
                     LFRNTRSEVRQGPRLIFGENKVLWLAALLFHWALFIILLRHLRFFLEPVPTFVNVISS
                     LDGFFQIGSPGLYLTDVMILGALGYLLWRRLRDPQVRYISLFVDYLALFLLLGLVISG
                     LLMRYILRIDTVAVKQLALGLVTLAPVLPKEVGPVFFVHLFLFSALAVVLPFSKLMHM
                     AGVFLSPTRNLANTNRRQRHVNPWNPPVKVHSYEEWEEEFRGKIKAAGLPLERE"
     gene            2389..4008
                     /gene="dsrK"
                     /locus_tag="AC16G0_14980"
     CDS             2389..4008
                     /gene="dsrK"
                     /locus_tag="AC16G0_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004093072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrK"
                     /protein_id="HIR5100957.1"
                     /translation="MAEHLPKPEALVELDFRPPRKDWRDPSVEFRKGVFCYSAAPKNV
                     EYLGLPNPRKWQPGDLDWKLPPDWQRIILEGMRERLEKFRSFRLFMDICVRCGACADK
                     CHFYIGSGDPKNMPVLRAELLRSVYRRYFTLAGRLCPGLAGGRELTQAVLKEWFYYFY
                     QCTECRRCSVFCPYGIDTAEVTMIGRELLNLVGCNITWVLEPAANCDRTGNHLGIQPH
                     GFKDSIDFAVDELEEMTGVRVEAPINRKGAEILFVMPSADYFGTPHYYTLLGYLMLFH
                     EVGLDYTLSAFASEGGNFGLFSSHEMIKRLNAKIYLEAKRLGVKWVMGGECGHMWRVL
                     HQYMDTMNGPADFLEVPVSPVTGTRFEHALSTKMIHICEFTADLIHHGRLRLDPRRND
                     HWTVTFHDSCNPSRAMGLLEEPRAILRSVCNHFHEMPENTIREQTFCCGSGSGLGTDE
                     NLEMRMRGGFPRANAVKYVRDKFGVNLVACICAIDKATLPSLLEYWAPGVEVAGIHEL
                     VGNALVLQGERERTTDLRGQALAGKEAQSHA"
     gene            4001..4396
                     /gene="dsrJ"
                     /locus_tag="AC16G0_14985"
     CDS             4001..4396
                     /gene="dsrJ"
                     /locus_tag="AC16G0_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrJ"
                     /protein_id="HIR5100958.1"
                     /translation="MRDRGLILGGLALFVGLMTSPVWFNLAAGTSAKGPELKLPPREK
                     HCVAPVEYMRTSHMVLLTAWREEVVRQSKRTFVAFDGTSHTISLTQTCLKCHGAKVDF
                     CDRCHQYAGVTPYCWDCHIDPKAIPEGSA"
     gene            4393..5157
                     /gene="dsrO"
                     /locus_tag="AC16G0_14990"
     CDS             4393..5157
                     /gene="dsrO"
                     /locus_tag="AC16G0_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrO"
                     /protein_id="HIR5100959.1"
                     /translation="MSLDRRTFLGVAGLSALAVAGRSALEALAGAQGQDRAGTQSPTR
                     WAMIIDPRKCAKAEGCTDCIAACDAAHNVPQLFDRAHEVKWIWREPFARAFPDQASPY
                     SEEALTDRPVLVFCNHCDNPPCVRVCPTQATWKRRDGIVMMDWHRCIGCRYCVAGCPY
                     GSRSFNLQDPRPHIKSLNPSFPTRTKGVVEKCNFCEERLARGEPPACVTACKEKALVF
                     GNLEDAGSAVRQLLRTRYAMRRRPELGTGPAIFYLV"
     gene            5173..>6298
                     /gene="dsrP"
                     /locus_tag="AC16G0_14995"
     CDS             5173..>6298
                     /gene="dsrP"
                     /locus_tag="AC16G0_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015260806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate reduction electron transfer complex
                     DsrMKJOP subunit DsrP"
                     /protein_id="HIR5100960.1"
                     /translation="MFEKAVVGSRRYWTWIGVLFAVMLAGSLCYLRQLHVGLAITGLS
                     RDVTWGLYIAQFTFLVGVAASAVMVVLPYYLHDWKVFGKITILGEFLAIAAVTMCLLF
                     IFVDMGQPMRVWNVLLYPTPTSMMFWDMLSLNGYLLLNAVITLVTLSAERKGVPPPGW
                     IRPVIILSIPWAISIHTVTAFLYSGLPGRSFWLTALLAPRFLASAFAAGPALLILLTL
                     AIRRLTTYDPGREPIQRLSLIVTYAMIINVFFVLMELFTVFYSQIPEHMEHFQYLFLG
                     LHGHAPLAAWMWTSVCLAVASLVFLITPRYRTNEQTLAVACGMVFLSLWIDKGLGLIV
                     GGFVPSPLGHVARYAPTLPEVSITLAIWAVGFLLVTVFYKI"
BASE COUNT         1068 a         2089 c         1936 g         1205 t
ORIGIN      
        1 tgaggagaga agagaatatg agtaccgttg tcttggcagg caaggagatg gaagtggacg
       61 aggacgggtt catccaggac ccagcgctgt gggatgagga ggtggcggct gccctggcaa
      121 aggcggagga agggatcgac gagatgacgc cggaccattg gaagctcgtg cactacctga
      181 ggaactatta cctccaattc cagatcgccc ccatggtccg caagctctgc aaggaaaccg
      241 gcttctcctt gcagaaggtc tatgacctgt tcccctctgg cccggccaag ggcgcgtgca
      301 aggtggcagg cctcccgaag ccgaccggct gcgtgtagaa cgccccatgg acttgatcag
      361 cgtgcgtctg gagcatgacc tggctttcca ggccacagtc cgggaccatc ggatcctcat
      421 ggacatgcgg ggacaccccc gcttcgccga tgcgggcccc tccccggtgg agctcctggt
      481 gatggccatc ggcgggtgca tcggcatgca cgtggcgttg ttctgtgagc agatgaacct
      541 ctcgccggcc ggtctgcgcg tggatctctc ctttgccctt gccgaccaga acgggcggaa
      601 gcgcgtgtcg agcgtctatg ccgaggtgga cgcgccagga atcccgaccg acctcctgcc
      661 cgcggcggag agggctgccc ggctctcgat cctgcccaac agcttcgtca agactccgga
      721 gctggacatc gaggtccgga gcggatcggg gcagtgagga tcatggcaat cgaggatgcc
      781 gcacccctgc tggatgccct gaaggcccgg aagggcgcca tcgtcaaagc ctggatcgcc
      841 cgaacgcttc acgcgtatcc ggaccacacc ttccgatttc tggatcagga aaaagaccca
      901 tttcgcaacc ccgtcggcca caccctcaag gagggcctgg cggcgctctt ggaagagctc
      961 ctgggaggga tgaacgccga gaagatcaca ccgctcctga acgacatcgt gcggatccgg
     1021 gcggtccagg actgcaccgc cgcccaggcc gtcgcctttc cctttctctt gaagaacgtc
     1081 attcgggaaa ccctgcattc ctcctcttgc cctcccctct ccccttccgg gggaaaggac
     1141 aaaggcgacg caggaagctg gtctgacgcc ctggcgcccc tggaagaccg gattgacgag
     1201 atggccctgc tggcttttga cctcttcatg cggtgccgcg agagcatgta tgaggtcagg
     1261 gtcaacgaga ccaagcgcag gctgtacctg ctgcaacgga tgcacgcgag cagcctgccc
     1321 tcggccgggg acgatcccgg cgttccctcg aaatcctgag ggggggaagt gggtgcacgt
     1381 gaggtgaggg gcatgcacgc ccagcaggcg cttgccgtgg tcatgatcct ggcggccctc
     1441 ggattcgtcg gggggcaggc aacttccgtc cggacgctct tcggagtcgt cgtgccgtgg
     1501 tcggccctcg ccgtcttcct cctcggcttc atcttccagg tcattcggtg gtcgcgctca
     1561 cccgtcccct tccgcatccc cacgacgtgc gggcagcaga agtcgctggc atggatccag
     1621 gcgagccggc tcgagaatcc cgcttccact ctgggcgtcc tcgggcggat ggtcctggag
     1681 atcggcctct tccgctcgct cttccgcaac accaggagcg aagttcgcca gggccccagg
     1741 ctgatcttcg gcgagaacaa ggtcctctgg ttggccgccc tcctctttca ctgggcgctc
     1801 ttcatcattc tgctccggca cctgcggttt ttcctggagc ccgttcccac gttcgtcaat
     1861 gtgatctcga gcctggacgg cttcttccaa atcgggtcac ccggtctcta cctgacggac
     1921 gtcatgattc tgggcgccct ggggtacctc ctctggcgga ggctccgcga tccccaggta
     1981 cgctacatct cgctcttcgt cgactatctt gccctcttcc tgctgctcgg cctcgtcatc
     2041 tcgggtctcc tgatgcgata catcctcagg attgacaccg tggccgtgaa gcagctcgcc
     2101 ctgggcctcg tgacactcgc gcccgtgctc cccaaagagg tggggccggt cttcttcgtc
     2161 cacctgttcc tcttcagcgc gctggcggtc gtccttcctt tcagcaagct gatgcacatg
     2221 gcgggcgtct tcctaagtcc caccaggaac ctggccaaca cgaaccgcag gcagcgacat
     2281 gtcaatccct ggaacccccc tgtcaaggtc cactcctacg aggaatggga ggaagagttc
     2341 cggggcaaga tcaaggccgc cgggttgccg ctggagaggg aatagaggat ggctgagcac
     2401 ctcccgaaac ccgaagccct cgtcgagctt gacttccgtc ctccgcggaa ggattggcgg
     2461 gacccctccg ttgaattccg gaagggagtc ttctgctaca gtgccgctcc gaagaacgtg
     2521 gaatacctgg ggctccccaa cccccggaaa tggcagcccg gcgacctgga ctggaagttg
     2581 cctccggatt ggcagcggat catcctggag ggcatgcggg agcgactgga gaaattccgc
     2641 tccttccgtc tcttcatgga catctgcgtg cgttgcgggg cctgcgccga caagtgtcat
     2701 ttctacatcg gatcgggcga tcccaagaac atgccggtcc tgcgggctga actcctccgg
     2761 tcggtctacc ggaggtattt cacgctggcc ggtcgtctct gtcccggact ggccggcgga
     2821 cgagagctca cccaggccgt gctcaaggag tggttctact acttctacca gtgcacggaa
     2881 tgccgccgct gctcggtctt ctgcccgtac ggaatcgaca cggcggaagt gaccatgatc
     2941 ggccgggaac tcctgaacct cgtgggctgc aacatcactt gggtcctgga acccgcggcg
     3001 aactgcgacc ggacggggaa tcatctgggc attcagcccc acggcttcaa ggacagcatc
     3061 gacttcgccg tggatgaact cgaggagatg acgggcgtca gggtcgaggc tcccatcaac
     3121 cggaaggggg ccgagatcct gttcgtgatg ccctccgccg actacttcgg gaccccccac
     3181 tactacaccc tcctgggcta cctgatgctc ttccacgagg tcggcctgga ctacaccctc
     3241 agcgccttcg cctccgaggg aggcaacttc ggtctcttca gttcccacga gatgatcaag
     3301 cggttgaacg ccaagatcta cctggaggcc aagcggctgg gggtgaaatg ggtcatgggg
     3361 ggcgagtgcg gccacatgtg gcgggtcctg caccagtaca tggacaccat gaacgggcct
     3421 gcggacttcc tcgaagtgcc cgtgtccccg gtcacgggga cccgcttcga gcacgcgctg
     3481 tccaccaaga tgattcacat ttgtgagttc accgcggacc tgatccacca cggcagactc
     3541 cggctggacc cgaggcggaa cgatcactgg accgtcacct ttcacgattc ctgcaaccca
     3601 tctcgggcca tgggactcct ggaggagcca cgcgccatcc tccggagcgt ctgcaaccac
     3661 tttcacgaga tgcccgagaa caccatccgg gagcagacgt tctgctgcgg cagcggctcc
     3721 ggcctcggga ccgatgagaa cctggagatg cgcatgcggg gtggattccc cagggccaac
     3781 gcggtcaagt acgtccgcga caagttcggc gtgaacctcg tggcctgcat ctgcgccatc
     3841 gacaaggcca cgcttccttc gctgctcgaa tactgggcgc cgggggtgga ggtggccggc
     3901 atccacgagc tggtcgggaa cgccttggtc ctccagggcg agcgggaacg gaccacggat
     3961 ctccggggcc aggccctggc gggcaaagag gcgcaatccc atgcgtgacc ggggtctgat
     4021 cctgggcggc ctcgccctct tcgtcgggct gatgacctcc ccggtgtggt tcaacctcgc
     4081 ggctgggacg agtgccaagg gccccgagct gaagcttccc ccacgagaga agcattgcgt
     4141 ggcccccgtg gaatacatgc ggacctcgca catggtcctg ctcacggcct ggcgggagga
     4201 ggtcgtgcgg caatccaaga ggaccttcgt cgccttcgac gggacgagcc acaccatcag
     4261 cctgacccag acctgcttga agtgccacgg ggccaaggtc gacttctgtg atcgctgtca
     4321 ccagtatgcg ggcgtcacac cctactgctg ggattgccac atcgatccca aggcgatccc
     4381 cgaggggagc gcatgagcct cgacaggcga accttcctgg gcgttgccgg actctcggcg
     4441 ctggcggtcg ccggacgctc cgcgttggag gctctggcgg gagcccaagg gcaggaccgc
     4501 gccggaacgc aaagtcccac gcgctgggcc atgatcatcg atccgcggaa atgtgcgaag
     4561 gccgaaggct gtacggactg catcgccgcg tgcgacgcgg cccacaacgt cccacagctt
     4621 ttcgaccgcg cccacgaagt caagtggatc tggagagagc cttttgccag ggcgttccct
     4681 gaccaggcta gcccgtactc cgaagaggcc ctcacggacc ggccggtcct ggtcttttgc
     4741 aaccactgcg acaacccgcc ctgcgtccgt gtctgtccca cccaggccac ctggaagcgg
     4801 cgggacggca tcgtcatgat ggactggcac cggtgcatcg gttgccgata ctgcgtggca
     4861 ggctgcccct atggctcccg cagctttaac ttgcaggatc ccaggccgca catcaagtcc
     4921 ttgaatccga gtttcccaac gcggaccaag ggcgtcgtgg agaagtgcaa cttttgcgaa
     4981 gagcggctgg ccagaggcga gccgccggcc tgcgtcacgg cttgcaaaga gaaggccctg
     5041 gtcttcggga acctggagga tgccggctcg gccgtccgcc aactcctccg gacccggtac
     5101 gccatgcggc gtaggcccga gcttgggacc gggccggcaa tcttttatct ggtgtgaggc
     5161 ccgccgatgt tcatgttcga gaaggcggtc gtcggcagcc ggcgctactg gacctggatc
     5221 ggggtcctct tcgccgtcat gctcgcaggc tccctgtgct atctccgtca gctgcacgtc
     5281 ggactggcca tcaccgggct gagccgggac gtgacgtggg gcctctacat cgcccagttc
     5341 accttcctcg tgggcgtggc ggcctccgca gtcatggtcg tcctccccta ttacctgcac
     5401 gactggaagg tgttcgggaa gatcacgatc ctgggcgagt tcctcgccat cgccgctgtg
     5461 accatgtgct tgctgttcat cttcgtggac atgggtcagc ccatgcgggt ctggaacgtc
     5521 ttgctgtacc ccacgccgac ctccatgatg ttttgggaca tgctctccct caacgggtac
     5581 ctcctgctga atgccgtaat caccctggtg acgctctccg ccgagcgcaa gggcgtgccc
     5641 ccgccaggct ggatcagacc cgtgatcatc ctgtccatcc cctgggccat cagcatccac
     5701 accgtgacag ccttcctgta tagcggtctc ccggggcgaa gcttctggct gaccgctctc
     5761 ctggcccccc gcttcctggc ctcggccttc gccgcgggtc cggcactgtt gatcctgctc
     5821 acgctggcca tccgcaggct gacgacctac gatcccggcc gcgagcccat ccagaggctc
     5881 tcgctgatcg tcacctacgc catgatcatc aacgtgttct tcgtcctgat ggaactcttc
     5941 accgtcttct acagccagat cccggagcac atggagcact tccagtacct gttcctgggg
     6001 ctgcacggcc acgcgcccct ggcggcatgg atgtggacct ccgtctgtct ggccgtggcg
     6061 tccctggtgt tcctcatcac cccccgctac cggacgaacg aacagactct ggcggttgcc
     6121 tgtgggatgg tcttcctgtc gctctggatc gacaagggcc tcggcctcat cgtgggggga
     6181 ttcgtgcctt ctccccttgg ccacgtggcc cgatatgccc cgactctccc ggaggtgtcc
     6241 atcaccctgg ccatctgggc cgtcggattc ctgctggtca ccgtcttcta caagatcg
//
LOCUS       DBNGZC010000868         5744 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2327545, whole genome shotgun sequence.
ACCESSION   DBNGZC010000868 DBNGZC010000000
VERSION     DBNGZC010000868.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2327545"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /gene="nikC"
                     /locus_tag="AC16G0_15000"
     CDS             <1..812
                     /gene="nikC"
                     /locus_tag="AC16G0_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002720992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nickel transporter permease"
                     /protein_id="HIR5100961.1"
                     /translation="RRNRLMVLGTGIILGLVLVAALAPRLATHDPYEQVLNDRLLAPS
                     MTHRFGTDSLGRDIYSRVLYGARVTLTIALLVAGISTPLGMLIGIVAGYLGGAVDEIL
                     MRLADIFLAFPRLILAIAFAAALGPGVDNAIIAIAIAQWPSYARLARAETLNVKNNDY
                     IQAMRVLGAGKARIMAGHILPLTLSSIIVRMSLDMGTIILTAAGLGFLGLGAQPPMPE
                     WGLMVSDGRQFLVDQWWVSTLPGLAILAVVMGFNLLGDGIRDVLDPRQRQA"
     gene            1017..2006
                     /locus_tag="AC16G0_15005"
     CDS             1017..2006
                     /locus_tag="AC16G0_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5100962.1"
                     /translation="MTDDLLLDVQDLKVEFPTLRGTIHALQGVTFQLHRRETLGLAGE
                     TGCGKSVTALAILRLVSHPGRISGGRILFEGRDLLAVGEADMRDLRGSQMSMILQEPK
                     MALNPVIRVGEQVAETLLIHDRTLSRGAARARAVEMLATLGIPDSQRIARRYPHELSG
                     GMAQRVMIAMMLVTRPKLLIADEPTSALDVTIQAQILELMKALKREIHTAMLLITHDL
                     GIMAETCDRVAVMYAGCVVEQGPVKTVLKRPQHPYTQGLLQTIPNDVPPDRPLFSIRG
                     QVPSLLRPPTGCAFAPRCERAMPECAEKVPPQYRLEAGHYAACFLHRNRGEAA"
     gene            2154..3152
                     /locus_tag="AC16G0_15010"
     CDS             2154..3152
                     /locus_tag="AC16G0_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018752096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5100963.1"
                     /translation="MSSNGTQALLEIRDLKVHFALQEGLLRKGHAVVQALSGVSLSLA
                     KGEVFGLVGESGCGKTTLGRAVVGLAPVLSGEIRFAGGSVIGLTGRAAKERARRIQLI
                     FQDPYSSLHPRKTVRDLVGVGLKLHGLARGYEIDGRVLQILERVGLGLDHLYRYAHEF
                     SGGQRQRIALARALILKPELLILDEPTSALDVSVQAQTLNLLKELRQQFDLTYLFISH
                     NLGAVRYMSRRIGVMYLGHIVEMGATEELFRRPQHPYTRVLLSSLPSLDPEAPATRQV
                     LTGEPPTPINPPPGCPFASRCPDAQDICTREKPVLLQRGTDPTHVCACHFPMTNYQ"
     gene            3239..4237
                     /locus_tag="AC16G0_15015"
     CDS             3239..4237
                     /locus_tag="AC16G0_15015"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR5100964.1"
                     /translation="MAIVVDAHSDLLSDILGRRAAGQKKVLESIWLPAMAAGKIDVRV
                     AAIFSGPQYVPELALRRALDEVAGFLDELEATSGAALCTSHDDILRDTSEGKRAFILG
                     MEGVEPLGSDLHLLSVFHRLGVRVLGITHALRNYAADGAFFEPKRTGLVGGLSDFGVE
                     LVEKAQAMGMLLDVSHLNDPGFWDLIKLSRAPVIASHSNCRALKDHPRNLTDDQIKAV
                     ADTGGVIGINSIARFVDPPDLPHLLDHLDRLVKIGGKEHVGLGLDFCYYLLEHKSPVE
                     RSGMRPRAYLSVDGLGGDHDVPKIPVLLAERGYSADTIDLIMGENFMRVFKAVLKA"
     gene            4304..4720
                     /locus_tag="AC16G0_15020"
     CDS             4304..4720
                     /locus_tag="AC16G0_15020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HIR5100965.1"
                     /translation="MIIRPFLPEDESAVIALWERCELTRPWNDPHLDIRRKREVRPDL
                     FLVGLLDGAVVASVMAGYEGHRGWINYLAVAPEHQGKGLGRAMMAEAERLLAQAGCPK
                     INLQVRSSNRKVVDFYRRLGYSVDEVVSMGKRLTGC"
     gene            complement(4890..5693)
                     /locus_tag="AC16G0_15025"
     CDS             complement(4890..5693)
                     /locus_tag="AC16G0_15025"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR5100966.1"
                     /translation="MRQNRLRELLTAGKPTLGTHLHSTWPAVVEAVGHSGVFDYVEFV
                     SEYGPYDLTALENLCRTAELFGLSSMIKVDQEPRKFLAQRGIGAGFQSVLFADCRSVA
                     DAQECLRAARPDTPEDLGTYGAGFRRHAYQFYGAPIEQAQTAYVKAIRDVVVALMIEK
                     KGAVEHLEEILSLPGVDMIQWGAADYTISTGRPGQWFSPETRAVEKRVFETALRMGVP
                     PRCEINSPDEAKQYLDMGVRHFCVGTDLLVWFDWCRKNGEAMRRALEGN"
BASE COUNT          958 a         1842 c         1901 g         1043 t
ORIGIN      
        1 ggcgccggaa tcggctcatg gttctgggga ccggcatcat ccttgggctg gtgctggtgg
       61 ccgccctggc gccacgcctc gccacgcatg atccctacga gcaagtcctg aacgatcggc
      121 tgctcgcccc gtcgatgacg cacaggttcg ggacagacag cttggggcgg gacatctatt
      181 cgcgggtgct gtatggggcc agggtgacgc tgaccatcgc tctgctggtg gcgggcatca
      241 gcacgccgct gggcatgctg atcgggatcg tggccggcta cctcggcggc gcggtggacg
      301 agattctcat gcggctggcc gatattttcc tggctttccc gcgactcatc ctggccatcg
      361 ccttcgcagc cgccctcggg ccgggggtgg acaacgccat catcgccatc gccatcgccc
      421 agtggccttc ctacgcgcgg ctggcccggg ccgagacatt gaacgtgaaa aacaacgact
      481 acattcaagc catgcgcgtg ctgggcgccg gcaaggcgcg gatcatggcg ggtcacatcc
      541 tgccgctcac gctatcctcg atcatcgtgc ggatgagtct ggacatgggc accatcatcc
      601 tcacggcggc cgggttgggt ttcctgggcc tgggggccca gccgcccatg ccggagtggg
      661 gcctgatggt gagcgacggc cgccagttcc tggtagacca gtggtgggtc tccaccttgc
      721 ccggcctggc catcttggcg gtggtgatgg gcttcaacct gctgggtgac ggcatccggg
      781 acgtcctgga ccccaggcag cggcaggcgt agcaggctgt tgaaaatggc ccatctgctg
      841 ggacccatcc tcaggatggg taccccgccc gcctcgccac tcgctgcggc gcatctccag
      901 tacgcctgga cccagcagac tgggtacccc ggcctacggc gggccgcctt gcatctggac
      961 cattttgaac agcctggaag aagagcgatc catcagtatc ccaacggggc gcggacgtga
     1021 ctgacgacct cttgctggat gtccaggacc tgaaggtcga gttccccaca ctgcgcggca
     1081 cgatccacgc gctgcagggg gtgacgttcc agctccatcg gcgcgagacg ctgggtctgg
     1141 ccggcgagac aggctgcggc aagtcggtga cggcactggc aatcctgcgg ctcgtgagcc
     1201 acccgggccg tatcagtggt ggtcgcatcc tcttcgaagg ccgggatctg ctggcggtcg
     1261 gcgaggccga catgcgggat ctccggggca gccagatgtc tatgatcctg caggagccca
     1321 agatggcgct gaacccggtc atccgggtgg gtgagcaggt ggccgagacc ctgctcatcc
     1381 acgatcgcac gctgtcccgg ggcgcggccc gggctcgggc ggtcgagatg ctggccacgc
     1441 tgggcatccc ggattcgcag cgaatcgccc ggcgctaccc ccatgagctc tccggcggca
     1501 tggcccagcg tgtcatgatc gccatgatgc tggtgacacg gccgaagctc ctcatcgccg
     1561 acgagcccac gtcggctctg gacgtgacga tccaggcgca gatcctggag ctgatgaagg
     1621 ccctcaagcg ggagattcac acggcgatgc tgctcatcac ccatgatctc ggcatcatgg
     1681 ctgaaacgtg cgaccgggtg gccgtcatgt acgcggggtg cgtggtggag cagggaccgg
     1741 tgaagacggt gctcaagcgc ccccaacatc cctacacgca ggggctcctc cagacaatcc
     1801 cgaacgacgt gcctccggac cgaccgctgt tttcgatccg ggggcaagtg cccagcctgc
     1861 tccggccccc cacgggctgc gcctttgccc cgcggtgcga gcgggcgatg ccggagtgcg
     1921 cggaaaaggt gccgccgcag taccggctgg aggccggcca ttacgcggcc tgctttctcc
     1981 atcgcaatcg aggcgaggcg gcatgattgg gaaagcgccc gaggatcttg atgatccgca
     2041 gattacgcag attacacaga tttcacaaac gaggttcgtt ccgaatataa tctgcggaat
     2101 atgcgaaatc tgcggataga ttgttccttc gggtgtcttg gtgggatgtc ctgatgtcga
     2161 gcaacggcac gcaggcgctc ctggagattc gcgacctcaa ggtgcacttc gccctgcagg
     2221 aagggctgct ccggaagggc cacgccgtcg tccaggcgct gagcggggtc tcgctctcgc
     2281 tcgccaaggg cgaggtcttc ggcctggtcg gggagtccgg gtgcggcaag accactctgg
     2341 ggcgcgccgt ggtgggcctg gcgccggtcc tctcgggtga gatccgattc gcgggtggga
     2401 gcgtcatcgg cctgaccggc cgtgccgcca aggaacgcgc gcgccgtatc cagctcatct
     2461 ttcaggatcc ctattcgtcg cttcaccccc ggaagaccgt ccgcgacttg gtgggcgtgg
     2521 gcctgaagct ccacggcctg gccagaggtt acgagattga cgggcgggtc ctccagatct
     2581 tggagagggt cgggctgggc ctcgaccatc tgtaccgcta cgcccacgag ttctcgggtg
     2641 ggcagcgcca gcgcatcgcg ctggcccgcg ccctcatcct caagcccgag ctcctgatcc
     2701 tggatgagcc cacctcggcc ctggacgtgt cggtgcaggc gcagacgctc aatctgctca
     2761 aggagctgcg gcagcagttc gacctgacct atctgttcat ctcccacaac ctgggggcgg
     2821 tccgctacat gagccggcgc atcggcgtga tgtacctggg ccacatcgtc gagatggggg
     2881 cgaccgagga gctgtttcgc cggccccagc acccgtacac ccgggtgctc ctctcgtcct
     2941 tgccatcgct ggacccggag gcgccggcca cgcgtcaggt cttgaccggc gagcccccca
     3001 cgcccatcaa cccgccgccg ggctgtccgt tcgcgtcgcg ctgtcccgac gcgcaggata
     3061 tttgcacgcg tgagaaaccg gtcctgctcc agcgggggac cgaccccaca cacgtctgcg
     3121 cctgtcactt tccaatgacc aattaccaat gaaccaatga ccaattgtgg agaagtcaag
     3181 cgcagtttcg taattggtca ttgggatttg gtcattggtc attcacctgg aggttccgat
     3241 ggctatcgtg gttgacgccc actctgatct gctgagcgac atcctgggcc ggcgcgcggc
     3301 cggtcagaag aaagtgctgg agtccatctg gctgcccgcc atggcggcgg gaaagatcga
     3361 cgtgcgggtg gccgccatct tcagcgggcc ccaatacgtg ccggagctgg ccttgcgcag
     3421 ggccctggat gaggtggccg ggttcctgga cgagctggag gcgacatccg gcgcagcgct
     3481 ctgcaccagt catgacgaca tcctgcgcga cacatccgaa ggcaagcggg ctttcatcct
     3541 ggggatggag ggcgtggagc cgctgggatc ggaccttcac ctcctaagcg tcttccacag
     3601 gctgggtgtc cgggtgctgg ggatcaccca cgcgctcagg aactatgccg cagacggcgc
     3661 attcttcgag ccgaagcgaa ccggtctcgt gggtggcctg agcgacttcg gcgtggagct
     3721 agtggagaaa gcccaggcca tggggatgct cctcgacgtc tcgcatctga acgaccctgg
     3781 cttctgggac ctgatcaagc tctcccgggc tccggtcatt gcctcacact caaactgccg
     3841 cgccctcaag gatcacccgc gcaacctcac ggacgaccag atcaaggccg tggcggacac
     3901 cggcggcgtc atcggcatca actccatcgc ccggttcgtg gacccgccgg acctgccgca
     3961 tctcttggac cacctggatc gtctggtcaa gatcggcggc aaagagcatg tggggctcgg
     4021 tctggatttc tgctactacc tgctggagca caagagtccg gtggagcgct ccggcatgcg
     4081 cccacgcgcc tacctgtcgg tggacgggct gggcggcgac cacgacgtcc ccaagatccc
     4141 cgtcctcctg gccgagcgcg gctactcggc cgacaccatc gacctgatca tgggtgagaa
     4201 cttcatgcgg gtgttcaagg cggtgctgaa ggcgtaaccg agccaggcag aggagtagtt
     4261 gtcaagccgt cgattgaggc cgcgctggaa agttatggtc cgtatgatca ttcgcccgtt
     4321 cctgcccgag gatgaatcgg ccgtcatcgc gctctgggag cggtgtgagc tgacgcggcc
     4381 ctggaacgat cctcacctgg acatccgccg gaagcgtgag gtccggcccg acctctttct
     4441 ggtcgggctg ctggatggcg ccgtggtggc gtcggtgatg gcagggtacg agggtcaccg
     4501 aggctggatc aactatctgg cggtagcgcc ggagcaccaa ggaaaggggc tcggtcgggc
     4561 gatgatggcg gaggcggagc gactgctcgc tcaggccggg tgtcccaaga tcaacttgca
     4621 ggtgcgctct tcgaatcgaa aggtggtcga cttctatcgg cggctggggt actccgtgga
     4681 cgaagtcgtc agcatgggca agcggctaac aggctgttga aaagggccca tctgcggcgt
     4741 tggtgggctc gggcgctcgt tgcggcgtac cgaaagtacg cctcactcgg ccccgggtac
     4801 ccatccggaa gatgggtgac gccgccttgc atctgggtcc ttttgaacag cctgggcaaa
     4861 aacgggtttc tcggcggcct ggtagacact caattcccct cgagggcccg tcgcatggcc
     4921 tctccgttct tccggcacca atcgaaccat accaggagat cggtcccaac gcagaagtgg
     4981 cgcacgccca tgtcgagata ctgcttggcc tcgtccggcg agttgatctc gcagcggggc
     5041 gggacaccca tccgcaaggc cgtctcgaag acgcgtttct cgacggctcg ggtctccggg
     5101 ctgaaccatt gccccgggcg tccggtgctg atggtgtagt cggcggcgcc ccactggatc
     5161 atgtccacgc cgggcaacga caggatctcc tcgaggtgct ccaccgcacc cttcttctcg
     5221 atcatcaggg cgacgacgac atcgcggatg gccttcacat aggcggtttg ggcctgctcg
     5281 atgggagcgc cgtagaactg gtaggcgtgt cgcctgaatc cggccccgta ggtgcccagg
     5341 tcttccggcg tgtcgggccg cgcagccctg agacactcct gcgcatcggc gacgctacgg
     5401 cagtccgcga atagcacgct ctggaagccc gcaccgattc cccgctgcgc caggaacttg
     5461 cgcggctcct gatccacctt gatcatcgag gagaggccga acagctcggc cgtccggcag
     5521 aggttctcca aggcggtcag atcgtatggc ccgtactcgg acacgaactc gacgtagtca
     5581 aacacgccgc tgtgtcccac ggcctcgacc acggccggcc acgtgctgtg gagatgggtg
     5641 ccgagggtgg gcttgcctgc cgtcaacaat tcacgcagcc tattctggcg cacggtctct
     5701 ctccatcact gagggtgtgc ggaacaagag gggtagctcg acgg
//
LOCUS       DBNGZC010000869         9418 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1638067, whole genome shotgun sequence.
ACCESSION   DBNGZC010000869 DBNGZC010000000
VERSION     DBNGZC010000869.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 9418)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9418)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9418
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1638067"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..940
                     /locus_tag="AC16G0_15030"
     CDS             113..940
                     /locus_tag="AC16G0_15030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100967.1"
                     /translation="MFRQYPAGALLAFVAVISIGLWPPTKARSAQGFAPQCALPFQGI
                     AVTHPIDQTCGLEGKAKSLTARGQNRTKNNLGATGSPVTISLEDLLKLQAAVVVARIP
                     FGQPNQLPPDRTRLTGLIETTSGQRIGEGSLVEFLGFLLEAHVSDPRTGETVNCDRPG
                     NESNDIHITLADRPVARLCDGFVAEMIPHFRPSSWSVRNLNTLRGRPLRIAGHLLFDA
                     GHQPCRQGRRVGSNPARASLWEIHPVYSAEVCSEADVQRCREGDSSLWTSLHEWAGR"
     gene            complement(1018..1728)
                     /locus_tag="AC16G0_15035"
     CDS             complement(1018..1728)
                     /locus_tag="AC16G0_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR5100968.1"
                     /translation="MEPNVFSTLTIGFLLGMEHALDADHVVAVSTMVSQHKSLRRASL
                     VGVVWGLGHTTTLFLVGLAVILFRLRIPGWLAQSMEFAVGLVLVALGGSIVKGYFSGR
                     VHAHPHRHGADTHLHFHSHAVAPGHDHEHPLLQHRRSLLVGMVHGLAGSAALMLLVLA
                     TIRTPALGLLYIFVFGAGSILGMLGISTLLGVPCVLTAERSAVLHHRLRIAVGSGSVV
                     YGVWILAQVGLIQGLFRF"
     gene            2343..3347
                     /locus_tag="AC16G0_15040"
     CDS             2343..3347
                     /locus_tag="AC16G0_15040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5100969.1"
                     /translation="MITTPRVLIVDDEAPVRRQLKVGLAQHGYEVEESEEGLEAIAKI
                     KTARATNPFRFVILDIRLPDIDGLKVLQAIKGTYPDLPVVVISGYGNESTPDTVERKK
                     GSLYLDKPFDVDTLIAEISKIGPSEGMEAKPLPEAAPEVKPLESAFVFIKAKADVDLY
                     EMYMKLCVAEGVCYCDPLVGDWDMVLLLQASNRKGIDQLVKAHLSRLKEVDSFELHHS
                     ERPRLPKEYETFMQDYERKQAAVRAEEGQVDKRKPKMLTAYAVLDVEPDRLSLLYMKL
                     CLTDSVVHCDVTDDGRKMILLLQADLIQEIQMAVRNEIRLMPGVLRIKVMNALNFWTK
                     "
     gene            3367..3918
                     /gene="nuoE"
                     /locus_tag="AC16G0_15045"
     CDS             3367..3918
                     /gene="nuoE"
                     /locus_tag="AC16G0_15045"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HIR5100970.1"
                     /translation="MARASEDENTNPTHAFGRMLWRHRGEPGMLIPLLQAAQDSYGHV
                     PPLAVEQISGVTGIPPSEVYGVITFYKQFRLKPVGRHLIRLCKGTACHVQNANMIAQT
                     VADELKIPPDETTEDGIFTFSVVACLGCCSLAPVLMVDDQTYGRMTPQKVRRVLRQYR
                     QRAASSPKTASPKPASAAVAANP"
     gene            3930..5678
                     /locus_tag="AC16G0_15050"
     CDS             3930..5678
                     /locus_tag="AC16G0_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014012577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HIR5100971.1"
                     /translation="MQEVVVGLGSCGVAAGGQLVHQRFAELFHADRNGSLLKQTGCIG
                     LCYREVLVEVRDRQGRTVLYGEVSPERVDRIVAEHLGQGKVIDEWVVDTAEVFARQQR
                     IVLRNCGVIDPSSLEEYCARDGYRALENVLTQMTPPQVVDLISESGLRGRGGAGFPTG
                     TKWRLAQATKDPHKYVVCNADEGDPGAFMDRSVLEGDPHSVLEGMAIAAYAIGADAGY
                     VYVRAEYPLAVRRLRQAIVQATKHGYLGANILGKGFHFEVALKEGAGAFVCGEETALI
                     ASIEGRRGMPRVRPPFPVEAGLWGHPTCINNVETLANVPWIVLNGAGAFRAYGTAGSK
                     GTKVFALAGKVKRGGLVEVAMGTTINEVIDEIGGGTTSGRRFKAVQMGGPSGGCIPAS
                     LGNTPVDYEQLTATGAIMGSGGMVVMDDSTCMVDVARFFLSFTQNESCGKCTFCRIGT
                     KRMLEILERICAGTGKDEDLNLLAELGEQIKASSLCGLGQTAPNPVLTTLRYFLPEYR
                     THITDKKCPAKVCLPLVDFFIDPAKCNGCGLCVETCEVGAVSGEKKLPHRIDVGVCVK
                     CARCLTVCRHGAIEKR"
     gene            5683..9369
                     /locus_tag="AC16G0_15055"
     CDS             5683..9369
                     /locus_tag="AC16G0_15055"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR5100972.1"
                     /translation="MLSHPAPQQSALPVELVINGRKVAARPGQTILDVVREGRLDEIP
                     TLCHDPRLQPYGSCFLCVVEVKGSPRLLPACVTRVRDGMEVTTRSERIIHVRKRALAL
                     LLSDHYADCVCPGQLACPAGVDVQGYISLARLGYYHEALALIRERNPLPVVCGRVCVR
                     KCELQCRRNAVDEPVGINFIKRYVSQHGDGEEARSSPVTPTGKRVAIVGGGPAGLTCA
                     NYLALDGHSVTIFEALPALGGMLRYGIPEYRLPRAELDEEINRILGLGIQVMPGKRLG
                     RDFSLESLIQRDKFDVVFLAVGAPLGKKLGVPGEEDAEGVLSALDFLRQVELQGPPRL
                     SGKVAVVGGGNSAIDAARTALRCGAEEVVILYRRTRKEMPAHHEEVEAAEKEGAQLEM
                     LVAPVAILAREGRLTGVRCIRMALGEPDASGRRTPVPIPGSEFDSACGLLLPAIGQDA
                     DPATFRNEPDRTRPAISRRTTLEADSSTLATNLAGVFAGGDVVSGPSAVIDAVAHGRM
                     AADAIDRYLRTGQVTGAPPPFVSRREVFGPLADWLFAGVTHASRQRMPEREPHERTKD
                     FGQVELGLSEPQLKDEAIRCMECGCKSVFSCELKRYATEYQVDMSRFAGAVRKHRVDA
                     SHPLITLDPNKCILCGRCVRACADLVGLSVLGFVGRGFGTQVGPALGRPLAESACIAC
                     GACVETCPTGALESRLPYGRQGPWKSRRHPSICGFCSLGCRLDVHIVSDGLLWASSPA
                     DSSDGGDLCLKGRFGTGLIYGADRLRQPLVRRDGRLVETDWDEAIHQAAAVLLECRNQ
                     EGPAALAVLAGARMTLEECFLTRHLARTALGAGQTGSFGQHRRGGPRHDLDGILGETA
                     STCVREDIDAADLVLLVGADPGVTHPVLAMAIRRAAKRGAEIAAIGSSKIDLIHSGDL
                     WLDARRGTAGIILAGVIRRIFQHGQIRQSILDGGQGGLEALYRSVADAAMEEVAAVSG
                     VEGSKIEALVERLAGRRRIVAIYDLDDTLERSVDDLIALTQLLWLTGHLGKPGEGLLL
                     LQSDCNSEGARLAGIAGALTPGTMRGALVIGENPFGDPDSRRQLERLRWIVAIDHCLT
                     ETAKAAQVVLPGATLVESEGTLVSFDRRVRGISRASQPVSGLGTAEVLGRLARELGHP
                     VRSTDPARIRVELAGQQGLAPEHLEEARDRGEIWPARRDPNRSLRPRAVRLDSAAMTA
                     DVYPYASLDAHVARRLVEMGLPRP"
BASE COUNT         1707 a         2925 c         3153 g         1633 t
ORIGIN      
        1 aaggagctgg ggatatccgt gccagggaag ggcagtgttg agccagagaa aggggaggga
       61 tgagccggtt cgacaggccg gaagtgaccg gtaggaatat gtgggaatct ctgtgttcag
      121 acagtacccg gcaggcgcgc tgctcgcatt cgtggcagtg atctcgatcg gcctgtggcc
      181 tccgaccaaa gcgcggtcgg cgcaggggtt tgcgccccag tgtgccctgc ccttccaggg
      241 aatcgccgtg acgcatccca tcgaccagac gtgtggactc gagggcaagg ccaagagtct
      301 tactgcacgg ggccaaaacc gcaccaagaa caacctcggc gcaaccggca gtccggtgac
      361 catcagcctg gaagatctcc tgaagctgca agcggcagtg gtggtggcgc ggataccatt
      421 tgggcagccg aaccagcttc cacccgaccg gacacgactc acggggctca tcgagaccac
      481 ctccggccag cggatcgggg agggaagtct cgttgagttc ctcggtttcc ttctcgaggc
      541 ccacgtgtcc gatccaagga ccggcgaaac cgtcaactgt gaccggccgg gcaacgagtc
      601 gaatgacatt cacatcacgc tggccgatag gcccgtggcg cggctgtgcg acggcttcgt
      661 ggcggagatg atcccccact tccgcccgtc atcctggagc gtaaggaacc tgaacacact
      721 ccgggggcgt cccctccgga tcgcagggca tctcctcttc gacgcaggcc accagccgtg
      781 caggcaaggg aggcgagtcg gctcgaatcc ggctcgtgcc tctctgtggg agattcaccc
      841 cgtctattca gccgaggtgt gctctgaagc tgacgtgcag cggtgcaggg aaggcgacag
      901 ctcgctgtgg acgtctttgc acgaatgggc cggccgttag cctcgagtcg gtgcgcgcta
      961 cgggaaacca gtggaatgac cgtgtggcca ctcgtgctcc ggtctccctc ccaggtatca
     1021 gaacctgaat agtccctgga tcaatccgac ctgtgccagg atccacaccc cataaacgac
     1081 gctgcccgag ccgacggcga tccgcagcct gtggtggagg accgcagacc gctcggcggt
     1141 caagacgcaa gggacgccga gcagggtgct gattcccagc atccccagga tcgatcccgc
     1201 tccgaagacg aaaatgtaca ggaggccgag cgccggggtt cgaatggtcg cgaggaccag
     1261 aagcatcagc gcggcgctgc ccgccagacc atgcaccatc ccgaccagca gcgaacggcg
     1321 gtgctgcagc aaggggtgct cgtgatcgtg tcccggagct acggcgtggg aatggaagtg
     1381 aaggtgggtg tccgcgccgt gccgatgcgg gtgggcgtgg acccggccgc tgaagtagcc
     1441 cttgacgatc gagcctccga gcgccacgag aacgagcccg acggcgaact ccatggactg
     1501 cgccagccat ccgggtatcc tgagccggaa caggataacc gccaggccga ccaggaatag
     1561 cgtggtggta tgccccagac cccagacgac ccccaccagc gaggcgcgcc tgaggctctt
     1621 atgctgactg accatggtcg agacggccac gacatgatcc gcatccaggg catgctccat
     1681 acccaggagg aaaccgatcg tcagcgtgct gaagacattt ggctccattc gcctcctccc
     1741 ctgcctgcct aatctctcga gacagtttac cggatgaacc tcggatttag aggaagaaac
     1801 gacacgccgg gccgaaaagg tcctctggtc cgtacctcga acagctcccg gtggggttgg
     1861 agccgtgggg cttcatccgg ggaaaatggc gtcccggccc tctcgcattc tcccaaaggc
     1921 cttgctgtta cgatcccatc ctggggttat tcggacctag acagttacga ctctgcctgc
     1981 acacggctga ttctgtaagt gaggcgaact cttcgagtca gattgtcgca cattgtccac
     2041 gcccgccata tccttgggac ttttttgggg agttttgccc cctcttcctg gtctgcccac
     2101 cctcagaagt attccgactg aatttgaatt ggcgaataat cccccaggcc gtctttcgat
     2161 ggagccgatg aatcctttgg tggcaatagg ttaaacgccg tccactcctt tgggaccctc
     2221 ctccgtcttc ctcccccgca ggtcttgcct caattcttca attggcatgg aagtggctta
     2281 tgacatcgta gacaaatcga cgccacaccg cacttggcac tccaaccagg aggaatgtca
     2341 ggatgattac cacaccaaga gtgctgatcg tggacgatga ggccccggtc cggcgccaac
     2401 tcaaggtcgg tcttgcccaa catgggtacg aggttgagga gagcgaggaa ggcctggagg
     2461 caatcgcgaa aatcaaaacc gcgcgcgcca cgaacccttt ccggttcgtc attcttgaca
     2521 tccgcctgcc tgatattgac ggcctcaagg tgctgcaagc catcaagggc acctatcctg
     2581 accttcccgt ggtggtgatt tccggctatg ggaacgagag cacgccagac accgtcgaac
     2641 gcaagaaggg cagtctatat ctggataagc ccttcgacgt ggacaccctg attgcggaga
     2701 tcagcaagat cggcccatcc gaggggatgg aagccaagcc actaccggag gccgccccgg
     2761 aagtcaaacc gcttgaaagc gcctttgtct tcatcaaggc caaggccgat gtcgacctgt
     2821 acgagatgta catgaagctc tgcgtcgccg agggcgtctg ttactgcgac ccgctggtcg
     2881 gcgactggga catggtcctc ctgctgcagg cttccaatcg gaagggcatc gatcaactcg
     2941 tcaaggccca cctttccagg ttgaaagagg tcgattcctt cgagctgcac cactccgaaa
     3001 ggcctcgcct gcccaaggaa tatgaaacct tcatgcagga ctacgagagg aagcaagcgg
     3061 cggtgcgggc cgaggagggt caggttgaca agcggaagcc caagatgctc acggcctatg
     3121 cggtcctgga tgtcgagcca gaccggctct cgcttctcta catgaagctc tgtctcactg
     3181 acagtgtggt tcactgcgac gtcaccgacg acgggcggaa gatgatcctc ctgctccagg
     3241 ccgacctcat ccaggaaatc cagatggccg ttcggaacga gatccgcctg atgcccgggg
     3301 tgctgcgcat caaggtgatg aacgcgttga atttctggac gaagtagctt caagacgggc
     3361 acgagaatgg cacgcgcatc cgaggacgag aacacgaacc ctacccatgc cttcgggcgg
     3421 atgttgtggc ggcatcgggg cgagccgggg atgctgatcc cgctgctgca ggcagcccag
     3481 gacagttacg gacatgttcc cccgcttgcc gtcgagcaga tctccggggt caccggcatt
     3541 cccccctcgg aggtctatgg tgtcatcacc ttctacaaac agttccgcct gaaacccgtt
     3601 ggacgacacc tgatccggct ctgcaagggc actgcctgcc acgtccaaaa cgccaacatg
     3661 attgctcaga cggtggcgga cgaactgaag attcctccgg atgagacgac cgaggatggg
     3721 attttcacct tcagcgtggt ggcctgcctt gggtgctgta gcctcgcccc ggtgctgatg
     3781 gtcgacgatc agacctacgg caggatgacc ccccagaaag tgcggcgagt cctccgccag
     3841 taccgccagc gcgccgcgtc ctccccgaag accgcgtcac ccaagccggc atccgcagcc
     3901 gtggctgcga atccttgacg gaggattgcg tgcaggaagt cgtggtcgga cttggttcct
     3961 gtggtgtagc cgccggtggc cagctcgtcc accagcgctt cgccgaactg ttccacgccg
     4021 accggaatgg ctcgctcctc aagcagaccg gctgcatcgg cctatgctac cgggaggtct
     4081 tggtcgaggt gcgggaccgc cagggacgga cagtcctgta cggtgaggtc tcgcccgagc
     4141 gggtggaccg gatcgtcgcc gaacacctgg gacagggtaa ggtcattgac gagtgggtcg
     4201 tcgacacggc tgaggtcttc gccaggcagc agcgcatcgt cctccgaaac tgcggcgtga
     4261 tcgatcccag cagcctcgag gaatactgcg cgcgagacgg ctaccgcgcg ctggagaatg
     4321 tcctcacgca aatgacgccc ccgcaggtcg ttgacctcat ctccgagtcc ggcctccgag
     4381 gacgtggggg agccggcttc cccaccggca cgaagtggcg actggcgcag gcgaccaagg
     4441 acccgcacaa gtacgtcgtc tgcaatgcgg acgaggggga cccgggcgca ttcatggacc
     4501 gcagcgtgct cgaaggcgac ccccattccg tcctggaagg gatggccatt gcagcctatg
     4561 ccatcggcgc tgacgcgggg tacgtgtacg tccgggcgga atacccgcta gccgtgcgac
     4621 ggctccggca ggcgatcgtg caggcgacaa agcatgggta cctgggcgcc aacatcctgg
     4681 ggaaggggtt ccacttcgag gtcgccctga aggaaggggc tggcgccttc gtgtgcggag
     4741 aggagaccgc cctcatcgct tccatcgagg ggaggcgggg catgccgcgg gtccggccgc
     4801 cgttccccgt cgaggcggga ctgtggggcc accccacctg catcaataac gtggagaccc
     4861 tggccaacgt cccgtggatt gtgttgaacg gcgccggcgc gttcagggcc tacgggacgg
     4921 cggggtccaa ggggaccaag gtatttgcgc tggccggaaa ggtcaagcgc ggggggttgg
     4981 tggaggtcgc catggggact accatcaacg aggtcatcga cgagatcggc ggcggcacca
     5041 cctcggggcg caggttcaag gcagtgcaga tgggcggccc gtcaggcgga tgcattcctg
     5101 cctctctggg gaacacgccg gtcgattacg aacagctcac cgccaccggt gccatcatgg
     5161 gctccggcgg aatggtggtc atggatgact ccacgtgcat ggtggacgtg gcgcgtttct
     5221 ttctgagctt cacccagaac gagtcctgcg gcaagtgcac cttctgccgg atcggaacca
     5281 agcggatgct ggagatcctc gagcgcatct gcgcgggcac gggcaaggat gaggacctga
     5341 atctgctggc cgagctgggg gaacagatca aagccagctc gctctgcggc ctgggccaga
     5401 cggcgccgaa tcctgtgctc acaaccctca ggtacttcct gcccgagtac cgaacccaca
     5461 tcaccgacaa gaaatgtccg gccaaggtct gtctgcccct ggtggatttt ttcatcgacc
     5521 ccgcgaagtg caacggctgc ggcctctgcg tggagacctg cgaggtcggg gccgtcagcg
     5581 gcgagaagaa gctcccgcac cgcatcgatg tcggcgtgtg cgtgaaatgt gcccggtgcc
     5641 tgaccgtatg ccgtcatggc gcgattgaga aaaggtagtc ccgtgctctc tcatccggct
     5701 ccccagcagt ccgcgcttcc ggtagagttg gtgatcaacg ggcgcaaggt ggccgcgcgt
     5761 ccgggccaga cgatcctgga cgtggtccgg gagggtcggc tggacgagat cccgaccctc
     5821 tgccacgatc cgcgactcca gccgtacggg tcctgctttc tctgcgtcgt ggaggtcaag
     5881 gggtcccctc ggctcctacc cgcctgcgtc acccgggtcc gtgacgggat ggaggtcacg
     5941 acgcggagtg agcgcatcat ccatgtccga aagcgggccc tggcgctgct cctatccgat
     6001 cattatgcgg actgcgtctg tcccggccaa ctggcttgcc cggcgggggt ggatgtccag
     6061 gggtatatca gcctggcgag actggggtac tatcacgagg cgctggcgct catccgggaa
     6121 cgcaatcccc tgcccgtggt ctgtggccgg gtctgcgtgc gcaagtgcga gctccagtgc
     6181 cgaagaaacg ccgtggacga gcccgtcggc atcaacttca tcaagcgcta tgtctcgcag
     6241 cacggagacg gggaggaggc acggtcctcc cccgtgacgc ctacggggaa acgggtggcg
     6301 atcgtgggtg ggggacctgc cggactcacc tgcgccaact acctggcctt ggacgggcat
     6361 tccgtcacca tcttcgaggc cttgcccgca ctgggcggca tgctccgcta cgggattccg
     6421 gaataccgcc tcccgcgggc cgagttggac gaggagatca atcggatcct cggcctcggg
     6481 atccaggtga tgccggggaa gaggctcggg cgggacttca gcctcgaaag cctgatccag
     6541 cgggacaagt tcgacgtggt cttcctggcg gtgggggccc ccctgggcaa gaagctggga
     6601 gttcccggcg aggaggatgc ggagggggtt ctgtcagccc tggacttctt gcgtcaggtg
     6661 gagctccagg gtcccccccg actttcgggg aaggtcgccg ttgtcggtgg agggaactcg
     6721 gccatcgatg ccgcccggac tgcgcttcgt tgtggtgcgg aggaggtggt gatcctctat
     6781 cgccggacgc gcaaggagat gccggcgcat cacgaggagg tggaggcggc ggagaaggag
     6841 ggggcgcagt tggagatgct ggtggccccg gtggcgatcc ttgccaggga gggacgcctc
     6901 accggggtcc ggtgcatccg gatggcgctg ggtgagccgg atgcgagtgg ccggcgcaca
     6961 ccggttccca tcccgggctc cgagttcgac tcggcctgcg gtcttcttct ccctgccatc
     7021 ggtcaggacg ccgatccagc gacattccgg aacgagcccg accggactcg accggcgatc
     7081 agccgccgga ccaccctgga ggccgattca tccacgctgg cgaccaacct cgccggcgtg
     7141 tttgcggggg gggacgtcgt gtcaggtccg tccgccgtga tcgacgccgt cgcgcatggc
     7201 cgtatggccg cggatgccat cgatcgttac ctgcgcacgg gccaggtgac gggagcgccc
     7261 ccgccatttg tgagtcgccg cgaggtattc ggcccgctgg cggactggtt gttcgcaggg
     7321 gtgacacacg cttcccgcca gcgcatgccg gagcgggagc ctcacgagcg gacgaaggat
     7381 ttcggccagg tggaactcgg cctgtcggag ccgcagttga aggacgaggc gatccggtgc
     7441 atggaatgcg gctgcaagtc ggtcttcagt tgcgagctga aacggtacgc gactgagtat
     7501 caggttgaca tgtcgcgatt cgccggcgcc gtccggaaac atcgcgtgga tgcaagccat
     7561 cccctcatca cgctcgatcc caacaagtgc atcctctgcg gccggtgtgt ccgggcctgt
     7621 gccgacctgg ttggcctctc cgtcttgggt tttgtcggcc ggggtttcgg aacgcaggtg
     7681 ggacccgccc tggggagacc cctggccgag agcgcctgca ttgcctgcgg ggcctgtgtc
     7741 gagacttgcc ccacgggtgc cctggagagc cgattgccgt acggcaggca gggcccctgg
     7801 aaatccagac ggcacccctc gatctgtgga ttctgttccc tcggctgccg cctggacgtc
     7861 cacatcgtgt ccgatggctt gttgtgggca agttctcctg cggactcgag cgacggcggc
     7921 gatctctgct tgaagggacg gttcggcacc ggactgattt acggcgcgga ccggcttcgc
     7981 cagccgctgg tgagaaggga cggccgactg gttgaaacgg actgggacga ggcgatccat
     8041 caggcggccg cggtcctgct cgaatgccgg aaccaggaag ggccggcagc cctggctgtc
     8101 ctggccggcg cccgcatgac cctggaggaa tgcttcctca cccggcatct ggccaggacg
     8161 gcgttgggag ccggccaaac cggcagcttc ggacagcatc gccgaggagg tccgcgccac
     8221 gatctggatg gcatcctggg agagacggca tccacgtgcg ttcgcgagga catcgatgcc
     8281 gcagacctgg tgctgctggt gggggccgac ccgggtgtga cccacccggt ccttgccatg
     8341 gccatccggc gagcggcgaa gcggggggcg gagatcgcgg cgatcggctc gagcaagatc
     8401 gacctgatcc actccggcga tctctggctg gacgcgcgac gcggcacagc cgggatcatc
     8461 ctggcaggtg tcattcgccg gatcttccag cacggacaga tcaggcaatc cattctcgac
     8521 ggcggccagg gcggtctgga ggcgctgtac cgctccgttg ccgacgcggc catggaggaa
     8581 gtggcggccg tctccggggt cgagggatcc aagatcgagg ccctggtgga gcgacttgcc
     8641 ggccgtcggc ggattgtggc gatttatgat ctggacgaca ccctcgaacg atccgtcgac
     8701 gacttgatcg ccctgaccca gcttctttgg ctcaccggcc acctgggtaa accgggcgag
     8761 ggtctgctct tgctgcagtc cgactgcaac agcgaagggg cgaggctggc cggaattgca
     8821 ggggccctca cgccggggac gatgcggggc gcgctggtga taggtgagaa cccattcggg
     8881 gatcctgact cgcgccgcca actggagcgc ctgcgctgga tcgtcgccat cgatcattgc
     8941 ctcaccgaga ctgccaaggc agcgcaggtg gtgttgccgg gggcgaccct ggtcgaatcc
     9001 gagggcacgc tggtcagctt cgaccgacgc gtgcggggaa tcagccgggc cagccagccc
     9061 gtcagcgggc tcggcacggc cgaggtcctc ggccggctgg cgagggagct tggccatcca
     9121 gtccgctcca ccgacccggc gcgtatccgg gtggagctgg cggggcaaca gggcctggcc
     9181 ccggagcatc tggaggaggc ccgcgatcga ggcgagatct ggcccgcgcg gcgagatccg
     9241 aaccgctcgc tccggcccag ggcggtccgg ctggactcgg cggcaatgac ggcggacgtc
     9301 tacccctatg cgagcctgga cgcgcacgta gctcgacggc tagtcgagat gggtttgcca
     9361 cggccgtgag ggcggcgcag ggccgaagac tcgcgaggac caggagggaa tgcgggag
//
LOCUS       DBNGZC010000870         6243 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2327596, whole genome shotgun sequence.
ACCESSION   DBNGZC010000870 DBNGZC010000000
VERSION     DBNGZC010000870.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6243)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6243
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2327596"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="AC16G0_15060"
     CDS             <1..258
                     /locus_tag="AC16G0_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5100973.1"
                     /translation="YQAWLAKDWPGWWENAQRDELLAKFDQESDPKKRFAIWEDVQKL
                     IWEDVPIVKIGDLNGLALASSKVKGLWPTYLMPYYNVWLEK"
     gene            328..1272
                     /locus_tag="AC16G0_15065"
     CDS             328..1272
                     /locus_tag="AC16G0_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5100974.1"
                     /translation="MTQYLIRRILALVPVMLVVATLVFVLMHLTPGDPVSAMLGMDAN
                     PADVQRMRVALGLDQPLHLQYAQWIGKVLHGNFGESIFLQIPVLVAVAERLEPTLLLT
                     LLAMAVAVGLGIPAGVISATRFGSLADQGFMSLALLGVSMPEFWLGLNLIFFVAVKLG
                     WFPVAGYVPVGTSWLATLRYLAMPAFCLGFVQSALIARMTRAVMLDVLSQDYIRTGHA
                     KGLSQRRVVYRHALKNALIPTLTVIGISFAILMGGTIVIETVFNIPGVGRLLIQSVLR
                     RDYPVIQGVVLLIAGAYVLINLLVDVAYAYLDPRIKYV"
     gene            1285..2187
                     /locus_tag="AC16G0_15070"
     CDS             1285..2187
                     /locus_tag="AC16G0_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5100975.1"
                     /translation="MSQSAAPRSAQQPLGDKSAWRQTFRMLLRRRIVVFGAGLVGAAF
                     LVALLAPFLTGHNPNELQIVSRLLPPSGANLFGTDSFGRDVYSRVVYGSRLSILVGAL
                     SVTIATIGGGLVGLLAGFYPRLDGPLMRIMDGIMAFPNIVLAIALMASLGPSVLNVIV
                     SLGFVYMPRVARVVRSGVLVVREALHVEAARALGAGDMRILRLNILPNCLSPIIVQAT
                     FIFAYAVLGEAALSFLGVGVPPYIASWGNILSEGRQFITQAPWITMFPGAAIMFTVLG
                     LNVVGDGLRDALDPRMRHVYGRLS"
     gene            complement(2184..2633)
                     /locus_tag="AC16G0_15075"
     CDS             complement(2184..2633)
                     /locus_tag="AC16G0_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HIR5100976.1"
                     /translation="MALEGEQEYGGSGYDVRSRLPGLGTASRVLLLVLGSLLSGCATM
                     PPAPSVMALPGFEKPLEQFHAEDTSCREWAAQRTQETAQRASSGQFYGSGARQQWYDM
                     AYLQCMYAKGNRIPGIPTGPPPPPPGAVTPPSPSPPAGVAPPPAQTP"
     gene            complement(2900..3931)
                     /locus_tag="AC16G0_15080"
     CDS             complement(2900..3931)
                     /locus_tag="AC16G0_15080"
                     /inference="COORDINATES: protein motif:HMM:NF024260.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR5100977.1"
                     /translation="MGSVDVLSDVLRVIRLSGAIFFSAEFRAPYALAEPPAPQLAARL
                     FPATECLALFHVLAQGDCWIVPEGEAPLHMEQGDVIIFPHSHPHVVTSRVGTVPTPLD
                     GLLPAAGADGILRMNHGGEGPATRFVCGYLHCDQRFNPLLGALPTFLWVHRRQSTTTL
                     ETPGHGPARTAVVLPPDAGAWLTTTLGYLLAEVAAEQPGGRAMLSRLAELLYVEVLRR
                     YVHHLPVGQTGWLAGLNDPQVGRALRLLHHDPARVWTVETVARAAGVSRSSLALRFTE
                     LIGDTPMHYLTAWRMHLARGMLKENRFTIPEVAARVGYHSEAAFNRAFKRVAGAPPGL
                     WLKREGPRP"
     gene            4122..4472
                     /locus_tag="AC16G0_15085"
     CDS             4122..4472
                     /locus_tag="AC16G0_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015146351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR5100978.1"
                     /translation="MASTKDVVDNHLKCFGEGDLTGILSDYAPGAVLFTSDGPLRGAD
                     AMRPLFQAMIAEFGKPGAAFSLKQQSVEGDYAYILWTAETADNVYEVATDTFIVRDGK
                     IVAQSFAGKITPKG"
     gene            4593..5978
                     /locus_tag="AC16G0_15090"
     CDS             4593..5978
                     /locus_tag="AC16G0_15090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5100979.1"
                     /translation="MTTLQARTFEELKGGFKGEILLPSDGAYDSARKIWNAMIDKHPA
                     VIARCVTTSDVVRGVNFARINRLLLAIRGGGHNIAGNAMCDDGLVIDLSRMKAAQVDP
                     ARRRVTIEGGATLADLDAATQAHGLATPVGINSTTGIAGLTLGGGFGWLSRKYGMTVD
                     NLESAEVVTAAGQVVRASATEHPDLFWALRGGGGNFGVVTRFEFRLHPVGPGVLSGLI
                     VYPISEAKSVLQQYREFLAKAPDELSVWTVLRKAPPLPFLPETVHGKEMIALALIYAG
                     DPKQGEPLIQPLRKFGTPLGEHVGVQPYVAWQQAFDPLLTAGARNYWKSHNFSTLKDE
                     LLDVVVAHVGKLPSPQCEIFFGAIGGATTRPAPDSTAYAHRDAKFVMNFHGRWEEPAD
                     DKRCIGWARDFFNATTPFASGGVYVNFLTADEDDRVRSAYGPNYDRLAQVKRKYDPDN
                     LFRMNQNIVPA"
BASE COUNT         1100 a         2060 c         1970 g         1113 t
ORIGIN      
        1 tatcaggcgt ggctggcgaa ggactggccc ggctggtggg agaacgcgca gcgggatgag
       61 ttgttggcca agttcgacca ggagtcggac cccaagaagc gcttcgcgat ctgggaggat
      121 gtccagaagc tgatctggga ggacgtgccc atcgtcaaga tcggcgacct gaacgggctg
      181 gccctcgcaa gcagcaaggt caaggggctc tggccgactt acctgatgcc ctactacaac
      241 gtctggctgg agaagtagct tccgtggccg ggtgacgcct ggcgcccacg gccgggcgtc
      301 tccccattcc ctggaaagct gccgcagatg actcagtacc tgatccggcg gatcctcgcc
      361 ctcgtaccgg tgatgctcgt cgtcgcgacg ctcgtcttcg tgctcatgca cctgacgccg
      421 ggcgacccgg tcagcgccat gctggggatg gacgcgaatc cggccgatgt gcagcggatg
      481 cgggtggccc tcggtctcga ccagccgctc cacttgcagt acgcgcagtg gatcggcaag
      541 gttctccatg gaaatttcgg cgagtcgatc ttcctgcaga ttccggtcct ggttgcggtg
      601 gcggagcgcc tcgagccgac gctcctgctg accctcctcg ccatggccgt cgcggtcggg
      661 ctcggcattc ccgccggggt catctcggcg actcggttcg ggagcctcgc ggaccaaggc
      721 ttcatgagcc tcgccctcct cggcgtctcg atgccagagt tttggctggg gctgaatctg
      781 atcttctttg ttgcggtgaa gctcggctgg ttcccggtcg ccggctacgt gcccgtcgga
      841 acaagttggc tcgcgacgct gcgctacctc gccatgccgg cgttctgcct gggcttcgtc
      901 cagtccgcac tgatcgcccg catgacccgg gcggtcatgc tggacgtgct gtcacaggac
      961 tacatccgca cagggcacgc caaggggttg tcacagaggc gggtggtcta caggcacgcc
     1021 ctaaagaatg cgctcatccc gacgctcacg gtcatcggca tcagcttcgc catcctcatg
     1081 ggcgggacga ttgtgatcga gacggtattc aacattcccg gagtcggccg actgctgatc
     1141 cagtctgtcc tgcgccgcga ctacccggtc atccagggcg tcgtgctctt gatcgccggc
     1201 gcctacgtgc tcatcaactt gctggtggat gtggcctacg cctacctcga cccgcgcatc
     1261 aagtacgtgt agcaggacga cgccgtgagc caatccgcag cgcctcgatc tgcccagcag
     1321 cccctaggag acaaatccgc ctggaggcaa accttccgga tgcttctgcg ccgccggatc
     1381 gtggtgttcg gtgcagggct ggttggggcc gcgttcctcg tggcgctcct cgcgccgttc
     1441 ctgacaggcc acaacccgaa cgagttacaa atcgtgtcgc ggctcctgcc tccaagcgga
     1501 gcgaacctct tcggcactga cagctttggc cgagacgtct acagtcgcgt ggtctatggc
     1561 tcccgtcttt caatactcgt cggcgccctg tcggtgacga tcgccacgat cggcggaggc
     1621 ctcgtcggtc tcctggccgg gttctacccc cggctcgacg gcccgctcat gcggatcatg
     1681 gatggcatca tggccttccc caacatcgtc ctggccattg cgctcatggc ctcgctcggc
     1741 ccgagcgtgc tcaacgtcat cgtttcgctc ggattcgtgt acatgccgcg ggtcgcgcgt
     1801 gtcgtccgaa gtggcgtcct ggttgtccgt gaggcgctgc acgtggaggc ggctcgggcg
     1861 ttgggcgctg gagacatgcg gatcctccga ctcaatatct tgccgaactg cctctcgccc
     1921 atcatcgtcc aggcgacgtt catcttcgcc tatgcggtgc tgggggaagc ggcgctgagc
     1981 ttcctcggcg tgggcgtccc gccgtacatc gccagctggg gcaacatttt gagcgaaggc
     2041 cgccagttca tcacccaggc gccctggatc accatgttcc ccggcgccgc catcatgttc
     2101 acggtcctgg gcctgaacgt ggtgggagac ggcttgcgag acgccctaga tccgcggatg
     2161 cggcatgtct acggtcgtct gtcttagggc gtctgcgcag gcggcggtgc gacaccggcg
     2221 gggggtgagg ggctgggcgg ggtaactgcg ccgggtggcg gaggaggagg accggtcggg
     2281 atcccaggga tccgattccc cttggcatac atgcactgca gataggccat gtcgtaccac
     2341 tgctgacgcg caccggagcc atagaactga ccggacgagg ccctctgggc cgtctcctgg
     2401 gtgcgctggg ccgcccactc ccgacaggaa gtgtcctcgg cgtggaattg ctccagcggt
     2461 ttttcgaacc cagggagcgc catcacgctc ggagccgggg gcatcgtggc gcacccgctc
     2521 aagagactgc ccaggacgag caggaggact cgcgaggccg ttcccaggcc tgggaggcgc
     2581 gaccggacat cataacctga tcccccgtac tcttgttcac cctcgagcgc cacccgtgtg
     2641 cccctccttg tctttacgat tacagaacaa ttcttgaccc tccggggttc caggggcaat
     2701 ggagacgccc cctggctcac gactcccgcg aatatgcgtg gaccccgata cgactcgctt
     2761 cccacggtct acgggaaggc gaagccattg tcatggcacg gctcaccgtg aatgtgacca
     2821 cgattctctc cgggagtatc ccgcctccgg tctacgagac gcagtccccc atcacccggt
     2881 ctgcgcgccc tgctgtcgat tacggccgcg gcccctcgcg cttcagccac aacccgggtg
     2941 gtgcccccgc cacccgcttg aacgcgcggt tgaaggcggc ctcagagtgg taccccaccc
     3001 gcgccgccac ctcgggaatg gtgaaccggt tctccttgag catccctcgc gccagatgca
     3061 tgcgccaggc ggtcaagtaa tgcatcggtg tatccccgat gagctcggta aatcgcagcg
     3121 ccagcgaaga acgcgacacc cccgcagcgc gagcaaccgt ctccacggtc cagacccgcg
     3181 ccgggtcatg gtggaggagc cgcaaggccc ggccgacctg ggggtcgttc agtcccgcca
     3241 gccatcccgt ctgccccacc ggcagatgat gcacatagcg ccgcagcact tcgacgtaga
     3301 gcaattcggc gaggcgggaa agcatcgccc ggcccccggg ttgttccgcg gcgacctcgg
     3361 cgaggagata ccccagcgtg gtcgtgagcc acgccccggc atcgggcggg aggacgacgg
     3421 ccgtccgcgc cggcccgtgg cccggggttt cgagcgtggt ggtgctctgt cgccggtgga
     3481 cccacaagaa ggtcggcagc gcgcccaaca acggattgaa gcgttggtcg cagtgcaggt
     3541 agccgcagac aaaccgtgtc gccggtccct cgccgccgtg gttcatccgg aggatgccgt
     3601 ccgccccggc ggctgggagc aacccgtcca gcggcgtggg cacggtgccc acccgactcg
     3661 tcacaacatg cgggtgacta tgcgggaaga tgatcacatc cccctgttcc atgtgcagcg
     3721 gcgcttcccc ttccgggaca atccagcagt ctccctgggc caacacatga aagagcgcga
     3781 ggcattcggt cgcggggaag agacgggctg cgagttgcgg cgcaggcggc tccgcgaggg
     3841 catacggcgc cctgaactcc gcgctgaaaa agatggcgcc ggagagccgg ataactcgca
     3901 gcacatccga gagtacgtcc acagacccca ctgcctcgcc gccaactggc cgcgcgttct
     3961 ggcgtggcga gcaagaatcc cggacgatgg ggccttgtgt caaggacgaa aatagtccag
     4021 attatgtgtg cactgcgggc cgcatcgcag ccggtgagca tctttgggcg aacgccaatg
     4081 gatcaaacga atagccagaa ctaacaacgg agggatgaga aatggcttcg acaaaagacg
     4141 ttgtagacaa ccatttgaag tgttttggtg agggcgacct gacgggcatc ctctcggact
     4201 atgcgccagg tgccgtcctg ttcacatcgg acggaccgct caggggagcc gacgcgatga
     4261 ggccgttgtt ccaggcgatg attgcggagt ttggaaaacc tggcgcagcg ttcagcctga
     4321 agcaacagtc cgtcgagggc gactatgcgt acattctgtg gaccgcagag actgctgaca
     4381 acgtgtacga ggtagccacg gacacgttca tcgtgcggga cggcaagatc gtggcccagt
     4441 ccttcgccgg caagattacg cccaagggct gaacacacgc cgtcattccg ggaggccgca
     4501 gaacaagccg atcacgcgga ccggttacga ccggcagctt atcggcaggt cgttagaagc
     4561 tattggccac aagacaggat aaggaggcaa ccatgactac tttgcaagcc aggacattcg
     4621 aggagttgaa agggggcttc aagggagaga tccttcttcc gagcgacggc gcctacgaca
     4681 gcgcccggaa gatctggaac gcgatgatcg acaagcaccc cgcggtcatc gctcgctgtg
     4741 tcaccacgtc ggacgtcgtc cgcggggtga acttcgcgag aatcaacagg ctcttgctcg
     4801 ccatccgcgg cggcgggcac aacatcgccg gcaatgccat gtgcgacgac gggctcgtga
     4861 tcgacctctc aaggatgaag gccgcccagg tcgaccccgc gaggcgccgc gttaccatcg
     4921 aaggcggtgc cacgctcgcg gacctcgacg ccgccaccca ggcccacggt ctcgccacgc
     4981 cggtcggcat caactccacc accggcatcg ccggattgac gctcggcggg ggcttcggct
     5041 ggctcagtcg caagtacggc atgactgttg acaacctcga gtccgccgag gttgtgaccg
     5101 ccgccggtca ggtggtgcgt gccagcgcca ccgagcaccc cgacctgttc tgggccctcc
     5161 ggggcggtgg cggcaacttc ggagtggtca cccgcttcga gttccgcctc catcctgtcg
     5221 gccctggcgt gctgagcgga ctcatcgtgt acccgatctc cgaggcgaag tcggtgcttc
     5281 aacagtaccg cgaattcctc gccaaggcac cagatgagct ctcagtgtgg actgtccttc
     5341 gtaaggcgcc gccactgccg ttcctccccg agacggtcca cggcaaggag atgatcgcgc
     5401 tcgcgctgat ttatgcgggt gacccgaagc agggtgagcc gctcatccag ccgttgcgaa
     5461 agttcggcac acccctgggc gaacacgtcg gcgtccagcc ttacgtcgcc tggcagcagg
     5521 ccttcgaccc gctgctcacc gccggcgccc gcaattactg gaagtcccac aacttctcga
     5581 cgctcaagga cgagctcctc gacgtcgtcg tcgcgcacgt cgggaagctg ccgtcacctc
     5641 agtgcgagat cttcttcggc gcaatcggcg gcgccaccac ccggccggcc ccggactcca
     5701 cagcctacgc ccaccgcgac gccaagtttg tcatgaactt ccacggccgc tgggaagagc
     5761 cggccgacga caagcgctgc atcggctggg cgcgcgactt cttcaatgcc acgacaccct
     5821 tcgccagcgg cggcgtctac gtcaattttc tcaccgccga cgaagacgat cgcgtgcgct
     5881 ccgcctacgg cccgaactac gatcgcctcg cccaggtgaa gcgcaagtac gacccggaca
     5941 acctctttcg catgaaccag aatatcgtgc cagcctgagg gaacatggcc gggctcccag
     6001 gctcggtcct cgcgcctggg agccctgtgg cctccagttc agttgagcct cctgatcccc
     6061 catcaatgtt cctgtaggac caacacctac acagatccgg gtcgaaatct gacaggagtc
     6121 gccgatggta tctgatagac cggacaggcc taatcaagta tcttcaggcg gatgtcgtct
     6181 ccggatggag actggcgatt ggcctagggg aaggatgcgg aataatacct gcggagagat
     6241 ccg
//
LOCUS       DBNGZC010000871         5335 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1345730, whole genome shotgun sequence.
ACCESSION   DBNGZC010000871 DBNGZC010000000
VERSION     DBNGZC010000871.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1345730"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..956)
                     /locus_tag="AC16G0_15095"
     CDS             complement(228..956)
                     /locus_tag="AC16G0_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013013400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5100980.1"
                     /translation="MDMLNLEGKVAVVTGGTRGIGRAIAATLSGQGVNVLICGRSGSE
                     AERVAAELTRHGPGKCVGTHCDVRRYEQVEAMMGQAAETFGGLDILVNNAGIAGKGAV
                     AEIPLELWHAVLDTNLTGVFHCCRAAIPWMRKRGGGFIINISSLAGVNPIPNLSAYNA
                     SKFGLLGFGEAFMQEIRYDGIRVSTILPGSVDTEFGGRSPGQDGWRLAPDDVAKVVVQ
                     LLAHDPRSLPSRVEIRPSMPPKKG"
     gene            1340..2221
                     /locus_tag="AC16G0_15100"
     CDS             1340..2221
                     /locus_tag="AC16G0_15100"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR5100981.1"
                     /translation="MDETKDWIANAREGISRRQFVTGAAAAGVSLAGFAIAAGPVAGQ
                     VITTSTEGLAVGESKVTSGNFQVPIYEARPAAGGQYPVVLVIPEIWGMHEHIKDITRR
                     FAKEGFISVTFEPYAREGGVLQIPDVPGVLKVVNTVPDARVMSDLDAIVAQIKQNPSV
                     RADRIGVTGFCRGGMYALLFAAHNHDMKAAVPWYGPIKPDKTPGIRDAGPLNVAAQIK
                     CPVLGLYGEADQGIPVADVKEMEAALKAAGTVAEFVEYPGAPHAFNADYRPSYRPEAA
                     KDGWARCVAWFDKYLKG"
     gene            2358..2969
                     /locus_tag="AC16G0_15105"
     CDS             2358..2969
                     /locus_tag="AC16G0_15105"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015195753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5100982.1"
                     /translation="MAFLLARFGGKHERYVADRKRALLSELSGDVLEIGPGTGVNLRY
                     YRNGIHWIGIEPNPFMHPYLREEAKRVGLKVEIRSGTAERLDSGDESVDAVVGTLVLC
                     SVVDMRGTLREIQRVLRPGGRFVFLEHVAAPPDTWLRRLQRLVRPVSRLLADGCCPDR
                     ETWKVLLGAGFAQVDYQSFRVPIPITAPHIAGVAVKRPAGATR"
     gene            complement(3001..4341)
                     /locus_tag="AC16G0_15110"
     CDS             complement(3001..4341)
                     /locus_tag="AC16G0_15110"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR02086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR5100983.1"
                     /translation="MGQTMVEKIVSRQVGRPVRAGEKVERLPVSKIFFNDVIGPPAIK
                     ILGNLFGETFKKFGKPVKVFDPRRIFLVPDHSVPALSVEVAEGVDLMETFGREQGMRM
                     YREGDGIEHVVLIEDGHIVPWDIVLGTDSHTCTNGAMGALAFGVGTTEGAYAMATGHI
                     YDFTVPETIQFETTGTFRKGVYSKDLILHIIGKLGAGGASRRVAEFVGDGVRRLSMDA
                     RTTICNMAVEMSARTGIMPYDETLGEYLKGRAQWPVQPIASDPDAEFADQVAVDLSAL
                     EPMIAFPHKPANATPVSHVAEMIARSQAQPTTDFPAVYTDRITDAFLGACTNARYEDL
                     VEAAQVLKGHRVHPDVTFIVIPASRKVYERALDEGILQVFIKAGANVESSNCGPCFGS
                     HMGVLSRKAQMISSSNRNYQGRMGSRDARIFLASPATVTASAIEGKLADPRRYL"
     gene            complement(4407..4931)
                     /locus_tag="AC16G0_15115"
     CDS             complement(4407..4931)
                     /locus_tag="AC16G0_15115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LeuD/DmdB family oxidoreductase small subunit"
                     /protein_id="HIR5100984.1"
                     /translation="MPSGSVTSVFGNDINTDDIIPAMYLIKSDDPEYFATYAFVKFDP
                     EFRNRCLQGGANIVVAGKNFGCGSSREQAVYALQFNRVVCVIAESFPDIFYRNSLATG
                     LPLVAMADRSGIGLGDKLDVDLEAGVVHNRTRNTTLPFQMKGDDRRTFLAGGMTARVR
                     AHLEEILAQGGQKA"
BASE COUNT          980 a         1713 c         1667 g          975 t
ORIGIN      
        1 ccaggacagc cgcacgtaac cacccgattc gctcggcgta atgtcgctcg aagtggtacc
       61 tagacattgg tagccccttt cgtctttcgc gtcgagtttc gccacagaac cgtaaggtcc
      121 atggccgcgc ttcagcccat cccgcgcatt gccatcctgg ccgacaattg atctcccgga
      181 tcaaacaacg gatgcgtgca ggccggcctc ccgaccggcc gtcgccctca ccccttcttc
      241 gggggcatgg acggtcggat ctccacgcga ctgggtaggg agcggggatc gtgggccagg
      301 agttggacca cgaccttggc cacgtcatcc ggtgcgagcc gccagccatc ctggccgggc
      361 gagcggccgc cgaactcggt gtccacgctg cccggcagga tcgtgctcac ccgaatgccg
      421 tcgtatcgga tctcctgcat gaaggcctcg ccgaatccca gcagaccgaa cttcgaggcg
      481 ttgtaggcgc tgaggtttgg gatgggattg acgccggcca gactgctgat gttgatgatg
      541 aatccgcctc cccgttttcg catccaaggg atcgcggcgc ggcagcagtg gaagaccccg
      601 gtgaggttcg tatccaggac ggcgtgccac agctccaggg ggatctcggc cacggcgccc
      661 ttgcccgcga tgccggcgtt gttcaccaag atgtccaggc cgccaaaagt ctccgcggcc
      721 tgccccatca tggcctccac ctgctcgtag cgccggacgt cgcagtgtgt gcccacgcac
      781 ttgccgggtc cgtggcgcgt gagttcggcc gcgacccgct cggcctcgga gccactgcga
      841 ccgcagatca agacattcac tccctgtccc gacagggtag cggcgatggc tcgaccgatg
      901 ccgcgcgtcc cgccggtgac gaccgccact tttccctcaa gattcagcat gtccatgtct
      961 tcctccccga ggtgtgtgtc gcgtgaacgc acacaggata gcaaaccccg cttaccctga
     1021 gcaagcaaaa cgccccgcgc attcgtcttg aaccgcacag cggactggcg gaagacccca
     1081 cgctaaccgc aaattacaga gattacgcag atctttcgaa ccccccagag ggcgaggggc
     1141 cgggtgagga tgacttctgc gtaacctgcg aaatctgtgg ctcatccccg aatttgtctc
     1201 ccggtctctg cggaagtcct acgaatccgg attgccaagc tgatgttggc tgtgtaggct
     1261 aagctgcctg ctggacccga gactggtctc tccctgaccc ctgacagacg aacgaccacc
     1321 ctcatccgag gaggactata tggacgaaac gaaggactgg attgccaacg cacgggaagg
     1381 catctctcga cgacagttcg tcaccggcgc cgccgccgcg ggggtgagcc tggcgggctt
     1441 cgccatcgct gcgggtcccg tggcgggcca ggtcatcacc acatccaccg agggcttggc
     1501 cgttggggag tcgaaggtga catcggggaa tttccaggtt cctatttacg aggcgcgtcc
     1561 cgcggcgggc gggcagtatc cagtcgtcct ggtcatccca gaaatctggg gtatgcacga
     1621 gcacatcaaa gacatcacgc gccgcttcgc caaggaagga ttcatcagcg taaccttcga
     1681 gccgtacgcg cgtgaaggag gcgttctcca gatcccggat gtgccggggg tcctgaaggt
     1741 agtgaacacc gtcccggacg cgcgggtcat gagcgacctg gacgctatcg tggcccagat
     1801 caagcagaat ccctccgtgc gggcggatcg gatcggcgtc acgggttttt gccgcggcgg
     1861 gatgtacgcg ctccttttcg cggcgcataa ccacgatatg aaggcggctg ttccttggta
     1921 cgggcccatc aagccggaca aaacgccagg catccgggac gcgggcccac tgaatgtcgc
     1981 agcgcagatc aagtgtcccg tcctcggcct gtacggggaa gccgaccagg gcatccctgt
     2041 ggccgacgtg aaggaaatgg aagccgccct caaggcggct gggaccgtgg ccgagttcgt
     2101 cgaatacccc ggcgcgcccc acgcctttaa cgccgattac cggcccagct accgacccga
     2161 ggcggcgaag gacggctggg cccgctgcgt ggcctggttc gacaaatatc tgaagggata
     2221 gtagggaacc cgaagcctgg aagagccgcc gggcctcccg gagctttggg aggcccgtgg
     2281 tatctcaatc accaatggtg cgccggacct cacgcagatc ggaaccccat aaccgggcga
     2341 cctggcggaa gtggttcatg gccttcctcc tggcgcgatt cggcgggaag cacgagcgct
     2401 acgtcgccga tcggaagcgg gcgcttctct ccgaactgag tggcgacgtc ctggagatcg
     2461 gacccgggac gggtgtgaat ctgcgctact atcgcaatgg catccactgg atcgggatcg
     2521 agccgaaccc attcatgcac ccgtatctcc gggaggaagc aaagcgggtc ggcctgaagg
     2581 tcgaaatccg cagcgggacg gccgaacggc tggatagtgg ggacgagagc gtggacgccg
     2641 tcgtaggcac gctggtgctc tgctcggtag tggacatgcg gggcacgctt cgggagatcc
     2701 agcgcgttct ccgtccgggc ggtcggttcg tcttcctcga gcatgtggcc gcgcctcccg
     2761 acacgtggct gcggcgtctt cagcgtctgg tccgaccggt gtcgaggcta ctcgcggatg
     2821 gatgctgccc ggaccgcgag acgtggaagg tgctcctagg tgcgggcttt gcgcaggtgg
     2881 attatcagag cttccgagtt cccatcccca tcaccgcgcc tcacatcgca ggcgtggccg
     2941 tcaagaggcc ggcgggagcc accagatgac ggatgagccg cgcctcccac gacggactat
     3001 ctagagatat cttcgcgggt cggcgagctt gccctcgatg gcgctggccg tgaccgtggc
     3061 ggggctggcc aggaagatcc gtgcgtcgcg agaccccatg cggccctgat aattccggtt
     3121 gctgctgctg atcatctggg ccttccggga gaggaccccc atgtggctgc cgaaacaggg
     3181 accgcagttc gaggattcca cgttggcccc ggccttgatg aagacctgaa gaatcccctc
     3241 gtccagggcg cgttcgtaca cctttcgact ggcgggaatc acgatgaagg tgacatccgg
     3301 atggacccgg tgtcccttca ggacctgtgc ggcctccacc aggtcctcgt agcgggcgtt
     3361 ggtgcaagcc cccaggaagg cgtcggtgat cctgtcggtg tagacggccg gaaaatcagt
     3421 tgtcggctgc gcctggcttc tcgcgatcat ctccgcgaca tgagacaccg gcgtggcgtt
     3481 ggccggcttg tggggaaagg cgatcatggg ctccagggcg gacaggtcca ccgccacctg
     3541 atcggcaaat tccgcgtccg ggtcgctggc gatgggctgg accggccact gcgcccgacc
     3601 cttcaggtat tcccccaggg tctcgtcata cggcatgatt ccggtccggg cgctcatctc
     3661 gacggccatg ttgcagatcg tggtgcgggc gtccatgctg agccgtctga ccccatcccc
     3721 gacaaactcg gccacccgcc gcgaagcccc gccggccccc agcttcccga tgatgtgcag
     3781 gatgagatcc ttggaataca cacccttccg gaaggttccg gtggtttcga actggatcgt
     3841 ttcagggacc gtaaagtcgt aaatgtgacc tgtggccatg gcataggccc cctccgtggt
     3901 ccccacgccg aaggccaggg cgcccatggc cccattggta caggtgtgag agtcggttcc
     3961 caggacgatg tcccagggaa cgatgtgtcc gtcctcgatc agcacaacat gctcaatccc
     4021 atccccctcc cggtacatcc tcatcccctg ctcccgcccg aacgtctcca tgagatccac
     4081 cccctcggcc acctccacag ataaagccgg cacggagtgg tcggggacca ggaaaatccg
     4141 acgggggtca aacaccttta cgggcttgcc gaacttcttg aaagtctcgc cgaagagatt
     4201 gccgaggatc ttgatggcag gtggcccgat gacatcgttg aagaatatct tgctgaccgg
     4261 caggcgctcg accttctcac cggcccgaac cggtcgaccg acctgccggg atacgatctt
     4321 ttcgaccatc gtctgtccca tggttccact ccgtttcacg tctaagaggc tggtgacccc
     4381 caggatgccg gtctctcgct ctgccttcag gccttctgtc ccccctgggc aaggatctcc
     4441 tccaggtgcg cccggacgcg ggcggtcatt ccgcctgcca ggaaggtccg gcgatcgtcc
     4501 cctttcatct ggaaagggag ggtcgtgttg cgtgtccggt tgtggaccac gccggcctcc
     4561 aggtccacgt cgagcttgtc tcccaggccg atgccgcttc gatcggccat ggcgaccagc
     4621 ggcaagccgg tcgccaagga gtttcggtag aagatgtccg ggaaggattc cgcgatgacg
     4681 cataccaccc ggttgaactg gagggcgtac acggcctgct cccggctgga accgcagcca
     4741 aaattctttc cggcgacgac gatattcgcc ccgccctgca gacagcgatt gcggaattcc
     4801 ggatcgaact tcacgaaggc gtaggtggcg aagtactccg gatcgtcgct cttgatcagg
     4861 tacatggcag ggatgatgtc atcggtgttg atgtcgttcc caaagaccga cgtgaccgac
     4921 ccgctcggca tggctcacac tccaagaaat gaatgtatcg aagggtcttc cgattccgca
     4981 gcctgcgaag cccgcgaggt tggatctcaa ggcaccagcc tcgcgtaatc tatcccaatt
     5041 gccgccgaag ctcaatcggg gatttcgaag ggcgacgggg aaggcagccg gtaccgattc
     5101 gcggatgctc tgtcatgcca ggcgtcctcg tccctacggc tccctcgccg tccattcgcc
     5161 gcagctattg cccaccgcct gcagcagtct aaggcccacc gcgcagaaag ggaacactga
     5221 ccagcagggt atagccattc agtgtgagca aggcgccgac gctcgcaacg gcaaggctga
     5281 tgatgaacac ggggcgcgac ccggagagga tcgagatgga tgacatcacg atggc
//
LOCUS       DBNGZC010000872         1873 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2327807, whole genome shotgun sequence.
ACCESSION   DBNGZC010000872 DBNGZC010000000
VERSION     DBNGZC010000872.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2327807"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1563
                     /locus_tag="AC16G0_15120"
     CDS             <1..1563
                     /locus_tag="AC16G0_15120"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HIR5100985.1"
                     /translation="ADDQILAPGFIDGHTHLDCIHRLDRFLEAAIPTGLTTVITEING
                     LSNVGGYPALAAFLALLPTLPITVLATAPTISYLLSDQGDGRSMLTMEEMARFLEEPG
                     VAGLGESYWPAVLEGRHDLDLLIAKAEAMGKPAEGHSAGARGVKLAAIAAAGISSCHE
                     PITPEEIRERLRLGLFTMVREGSIRRDAGALKDALTGIDSRRLMLASDTVWPHDLLAR
                     GYLDEAARQAVAAGLTPMQALQAITLTPAEHFRIEGRVGGLAPGRQADLVLLPDLSTF
                     RPSLVMARGRVVAQERQTVVPIPPVELSPEVLPAPRLPHPLGVDDLRIPAPAGRDQVQ
                     VRVIHYSSEIVTQAQIRTLPVREGALQAAPTTDLLKVVVLDRHGRGRVARGFVSGYGL
                     RRGAIASSLSFDTSNLILLGASDADMLVAAGRMRALNGGVVVAADGAIRAEIPLPLGG
                     IISDQPMTPLAAQIVSLQQVLHELGCVRDNPFLSAQVVTFTAIPALRIRERGLWDVRQ
                     SRVVPLLVDEGT"
     gene            1574..>1873
                     /locus_tag="AC16G0_15125"
     CDS             1574..>1873
                     /locus_tag="AC16G0_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100986.1"
                     /translation="MPNREKPVPIDEEILERLNRGILLRTGAYRTDEHIVLRSGRHTS
                     EYVAKALVTTEPTFTEGLGDIIAAHFSRYEVDLVLTKGYGAGLLVHCVARAHPSRP"
BASE COUNT          293 a          668 c          590 g          322 t
ORIGIN      
        1 gcggacgacc agatcctcgc cccgggcttc attgacggcc atacccacct ggactgcatc
       61 caccgcctgg atcgcttcct cgaggccgcc atccccacgg gcctgacgac ggtgatcacc
      121 gagatcaacg gcctcagcaa cgtgggcggc tacccggccc tggccgcctt cctggccctg
      181 cttcccacct tgcccatcac cgttcttgcg acggcgccga cgatctcgta cctgctctcc
      241 gaccagggtg acggccgatc catgctcacc atggaggaga tggctcgctt cctggaggag
      301 cccggcgtgg ccgggctggg ggagagctac tggccggctg ttctagaggg gcgacacgac
      361 ctcgaccttt tgatagccaa ggctgaggcc atgggcaagc cggcagaggg acactccgcc
      421 ggtgctcggg gagtcaaact cgccgccatc gcggccgccg ggatttcctc ctgccacgag
      481 cccatcacgc ccgaggaaat ccgcgagcgc ctgcgcctcg gtctcttcac catggtccgg
      541 gaaggctcca tccggcgcga cgccggtgcc ttgaaggacg ccctcaccgg gatcgattcc
      601 cgccggctca tgctagcctc agacacggtc tggccgcatg acttgctcgc ccggggatac
      661 ctcgacgagg cggcgcgcca agcagttgcc gccgggctga cacccatgca ggccttgcag
      721 gcgatcaccc taacaccggc agaacacttc cggatcgaag gacgcgtggg cggcttggct
      781 ccgggccgtc aggcggatct cgtcttactc cccgaccttt ccacattccg cccgagcctg
      841 gtcatggccc gcggtcgggt cgtggctcag gaaaggcaaa ccgtcgtccc gatccccccg
      901 gtagaacttt ctcctgaggt cctcccggcg ccgcggctgc cgcaccccct cggcgtggac
      961 gacctgagga ttccggcccc ggccggtcgg gaccaggttc aggtgcgcgt catacactat
     1021 tcctccgaga tcgtgaccca ggcccagatc cgcacgttgc cggtgcgaga gggtgccctc
     1081 caggccgccc ccacaaccga tctcctcaag gttgtggttc tggaccgaca cggtcggggg
     1141 cgagtggccc gcggattcgt ctccggctat ggccttcgcc gcggggcgat tgcctcgagc
     1201 ctgagctttg atacttccaa cctgatcctc ctcggcgcca gcgatgccga catgctggtc
     1261 gcggcggggc gcatgcgtgc actgaacggc ggcgtggtgg tggcagccga tggggccatc
     1321 cgggcggaaa tcccccttcc cctaggtggg atcatctcag atcagccgat gacgcccctg
     1381 gctgcccaga tcgtatcgct ccagcaggtg ctgcacgagt tgggctgcgt ccgagacaat
     1441 ccattcctga gtgctcaggt ggtcaccttc actgccatcc ccgccctccg aatccgcgag
     1501 cgcgggctgt gggatgtgcg gcagagccgg gtggtcccct tgctcgtgga cgaggggacc
     1561 tagagggacg cccatgccaa atcgcgagaa gccggtcccc atcgacgagg agatcttgga
     1621 gcgcttgaac cggggcattc tcttgcggac gggcgcgtac cggaccgatg aacacatcgt
     1681 cctccggtcg ggacgccata cgtccgagta cgtggccaag gccctggtca ccaccgagcc
     1741 gaccttcacg gaagggcttg gcgacatcat tgccgcgcac ttcagccggt atgaagtgga
     1801 tctcgtgttg accaagggct acggcgccgg cctcctcgtc cactgcgtgg cccgggccca
     1861 cccgtcccgc ccc
//
LOCUS       DBNGZC010000873         1464 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2422262, whole genome shotgun sequence.
ACCESSION   DBNGZC010000873 DBNGZC010000000
VERSION     DBNGZC010000873.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1464)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2422262"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="AC16G0_15130"
     CDS             <1..258
                     /locus_tag="AC16G0_15130"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR5100987.1"
                     /translation="PERVILFGSHARGEPAADVDVVIIKETTDRFLDRLKHVYERWSL
                     PVAVDILVYTPAEWTRMVQEERGFILRVLQEGREIYARSCG"
     gene            239..664
                     /locus_tag="AC16G0_15135"
     CDS             239..664
                     /locus_tag="AC16G0_15135"
                     /inference="COORDINATES: protein motif:HMM:NF017020.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HIR5100988.1"
                     /translation="MHDPAAEAHRWWSQALNDRSFVHHMAREALYFDKGCFIAQQAGE
                     KALKACLYAEGRREVLGHALVEFVGDLARREGAFGAVAAQAARLDRYYIPARYPNGLP
                     GGTPFETYTAIDLAAAIADLDAVFAVAEQFLRARQVVKD"
     gene            699..1319
                     /locus_tag="AC16G0_15140"
     CDS             699..1319
                     /locus_tag="AC16G0_15140"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HIR5100989.1"
                     /translation="MRILAIGAHPDDIEFGCGGTLIKYARQGHEVFLLVMTDGGHGGD
                     AVIRRAEQEASARILRASKLFWGEYPDTAVSLDRTLIHRIEGIIQEVRPEFILVHYGD
                     DTHQDHRNLSTSTVTATRYTRNVLFYEGPTTQNFSPSVFVDTGSVLEDKIRSLQAHAS
                     QVGKTNIEGLSILDIARSSSHFRGIQGRVRDAEGFIPLRLFINIQP"
BASE COUNT          262 a          469 c          471 g          262 t
ORIGIN      
        1 ccggagcggg tcatcctctt cggctcgcac gcgcggggcg agccggcggc cgacgtcgac
       61 gtggtcatca tcaaggagac gacagaccgc ttcctggatc ggctcaagca tgtctatgag
      121 cgttggagcc tgccggtggc cgtggacatt cttgtgtaca caccggcaga gtggacgcgg
      181 atggttcagg aggagcgagg attcatccta cgggtcttgc aggagggaag agaaatctat
      241 gcacgatcct gcggctgagg cccatcgctg gtggtctcag gccctgaacg atcgctcctt
      301 cgtgcaccat atggctcgag aggcccttta cttcgataaa ggctgcttta tcgcccagca
      361 ggcgggggag aaggccctga aggcctgcct ctacgcagaa ggacggcggg aagtgctggg
      421 gcacgccttg gtggagttcg tgggagacct ggcgagacgc gagggggcgt ttggagccgt
      481 ggccgcccag gcggctcgcc tggaccgcta ttacatccct gcccgctacc ccaatggcct
      541 cccgggggga acccccttcg aaacctacac ggcaatcgat ctcgcggccg cgatcgcgga
      601 cttggatgcg gtctttgcgg tggcagaaca attcctgagg gctcgtcaag ttgtgaaaga
      661 ctgagggtcc gtacccattc tcctcggagg ctgagctcat gcgcatcctg gccatcggcg
      721 cccacccgga cgacatcgag ttcggctgcg gcgggaccct gatcaaatac gcacggcagg
      781 ggcacgaggt cttcctgttg gtcatgaccg atggcggcca cggcggagac gcggtgattc
      841 gccgcgccga gcaggaggcg tccgcccgca tcctccgggc gtccaagctg ttctggggag
      901 agtatccgga caccgccgtc tcactcgacc ggacactcat ccaccggatc gagggtatca
      961 tccaagaggt gcggcccgag ttcatcctcg tccattacgg cgatgatact caccaggacc
     1021 accgcaacct ctccacctcc acggtgaccg ccacgcggta cacgcggaac gtcctcttct
     1081 acgaaggccc caccacgcag aacttctccc ccagcgtttt tgtggatacc ggcagcgttc
     1141 tggaagacaa gatcagatct ctccaggccc acgcctccca ggtgggcaag acgaacatcg
     1201 agggactttc catcctggac atcgcccgct cctcctccca ctttcggggg attcagggcc
     1261 gggtgcgcga tgccgaagga ttcatccccc tccggctgtt catcaacatt caaccctgat
     1321 ggctcgagag ctgacggcgc ccatccccca ttcccgtccg tggatcgccc caggcgacgt
     1381 gcaggcggtc tcccgcgtcg tgaggggcgg tgcgctggcc cagggggcgg aggtagcggc
     1441 gttcgaggcg gaggtcggcg gaag
//
LOCUS       DBNGZC010000874         5827 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2715623, whole genome shotgun sequence.
ACCESSION   DBNGZC010000874 DBNGZC010000000
VERSION     DBNGZC010000874.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5827)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2715623"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="AC16G0_15145"
     CDS             <1..281
                     /locus_tag="AC16G0_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100990.1"
                     /translation="IPAEPGAVAFLGAADLATKFPQVSTHKLSLGTLAKMIETTSRSR
                     VWLLGTQPKAFLPGAGLSQSVSTTLEALKALLCEVLAVRPAEPALAWE"
     gene            284..2227
                     /locus_tag="AC16G0_15150"
     CDS             284..2227
                     /locus_tag="AC16G0_15150"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter transmembrane
                     domain-containing protein"
                     /protein_id="HIR5100991.1"
                     /translation="MSAANAILLTLIVCAVGAALALAASRSKAAAGWLAFLTTSLASA
                     LALYAAGTVLVSGPGEALTLWALPQYGSALRIAVDGLSAVFVSLIAVISLLAALYSIR
                     YMEHYPDYGAARYYPYFLLFVAGMYGIVCLTDLMLFFFLFWQLMTLTSYALVRYEYRK
                     PENVRAAYRYLLMMQIACGLVMLGAGLLGLNPVVLRGSERLMTYDFDAIAHGVPGLLQ
                     QGGGAVALAFLCFLLGFGIKAGMWPFGQMWLPDAHPAAPSPVSALLSGVMIKTGIYGL
                     MRAFLWLIPPAAAAEYPSGGWGLLIAGLGTITLFVGTMQAMRQEQSKRLLAFHSIGQI
                     GYILLGLGTCLALLPAGLKSEAVLALATLGFYGALFHTVNHGIFKSLLFLNAGSILTA
                     TETQDLNKLGGLMKFMPATAVMTLIASCSIAGVPLFNGFASKWSLYVAAILGSGHAGY
                     LAVCAVIAIFTSAITLASFLKFFGGSFLSRTSSLVAGRAISRGRLEVGWLMQIPQVVL
                     AALCLILGILPLSAFWLFQTAMDRSQQGLGTLLAKAPLATVSGLTGIEGVGGRAVFAP
                     LALLLIFGGTLLLAVWVSRLGEAKRRVAEPWLCGYIQENEQNRYGAHNLYREITRHFR
                     WLGGMPRPRHQMGSSTGSTSRNA"
     gene            2270..3952
                     /locus_tag="AC16G0_15155"
     CDS             2270..3952
                     /locus_tag="AC16G0_15155"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HIR5100992.1"
                     /translation="MAAQKILNQLKEQFAPAIQRADVPADNRLFLYVQPEAMKGVCEY
                     VFRTLDARYVVGIGVDDRPYSGEFLVGHNFAFDSAHVLSSILTCLPGDRPELQSISDV
                     VPPANWSEREFRDLVGIVPVGHPSPKRLILPDGWPEGAHPLRKDVPWNFVPPGYDENQ
                     SFAFDDPPEGCVVVPVGPFHTTLDEPAHFRLFVDGETVRGCEYRGFMVHRAIEKLGDS
                     VLTYNEIPMAAERICGICGCVHNVAYAQAVEQAAAITPSPRAEYVRTIMLEVERLHSH
                     LLWVGLACHILGFDTLFMQSFRIREPIMWIAEKISGNRKTYALCVIGGVRWDITPELK
                     GELRRVLDTLEKEWIAVADAVRGDRDVQKRTRGVGVADKALVKNAGLIGPIARASGVG
                     IDVRRDHPYAAYDRVDFDVVVQDGGDVWSRILVRVLEVFESIKIIRQCLEKMPEGPLQ
                     TEVRDDLPVGRLGLSSVEAPRGESHHFVITGEDNRPRRWRVRAPTYQNLQGVPAMIKD
                     QQLADMTISLGSIDPCFSCTDRLEILDIRSGAQRVYSQEELLQLTRKGRPQE"
     gene            3977..4903
                     /locus_tag="AC16G0_15160"
     CDS             3977..4903
                     /locus_tag="AC16G0_15160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory chain complex I subunit 1 family
                     protein"
                     /protein_id="HIR5100993.1"
                     /translation="MGSGLVLSLATGVANVALVLLVAPFFEGVLRKVTAKVQSRQGPP
                     LWQPYYDLLKLLGKEDIESGEVPGMQRAAAYLSFAAILTVATLVPMGAPAPLRGAGDA
                     VLLIYLLTLCGISTLLAGLAAGSTYSLLGMSREMMAMMTLEPLLAVALVIGAVHTGSL
                     RLEVVLDGSAYTSGTIPWAGMIMAGVLLLSLQAHLGRVPFDICEAETEIMEGPMAEYS
                     GPKLALFKYVRMAKLVVYSALFVALFVPWGSHGPYPVNLLVLLVKILGLVLFVTLVAA
                     THARYRIDQAIRYYAGLFAASMLALGLAVSGF"
     gene            4921..5265
                     /locus_tag="AC16G0_15165"
     CDS             4921..5265
                     /locus_tag="AC16G0_15165"
                     /inference="COORDINATES: protein motif:HMM:NF024634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5100994.1"
                     /translation="MFLLSKFKEAAICLKAGRVTLPYPVEPRPVPKGFRGRPIFDAAK
                     CIGCAGCANNCPAREILVLDVCQEIRIIKYLGRRCTYCGRCADVCPEKAISMSPEFET
                     ATNDIHDISQEL"
     gene            5272..5445
                     /locus_tag="AC16G0_15170"
     CDS             5272..5445
                     /locus_tag="AC16G0_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5100995.1"
                     /translation="MGTCQRCGRCFKEPGPLEQLKMKGYRFDLLKEDRWVYRSKAYLD
                     DQPVVRDIDIELD"
     gene            5467..>5827
                     /locus_tag="AC16G0_15175"
     CDS             5467..>5827
                     /locus_tag="AC16G0_15175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIR5100996.1"
                     /translation="MLKELCQKVFPRSLWVYHANSGGCNGCDIEVLNVLTPYYDVERF
                     GIKLVGSPRHADVMLCQGPAMRSTAKALRRAYEAMPQPKLVFAIGSCACGGGIWHDTY
                     SVLGPVEKVLPVNFYIPG"
BASE COUNT          878 a         1937 c         1934 g         1078 t
ORIGIN      
        1 gtattccggc cgaaccgggc gcggttgcct ttttgggtgc ggctgacctg gcgacgaagt
       61 tcccccaggt ctccacccac aagctgtcct tgggcacgct ggccaaaatg atcgagacca
      121 caagccggtc cagggtgtgg ctgttgggga cccagccaaa ggcgttcttg ccgggcgccg
      181 gcttgagcca gtctgtcagc acgaccctgg aagccttgaa ggctctgctc tgcgaggtgc
      241 tggctgtccg gccagcagaa cctgcgttgg cgtgggagtg attatgagtg cggcgaacgc
      301 catcctcttg accttgatcg tgtgcgcggt cggcgcggcc ctggccctgg ccgcttctcg
      361 cagcaaggcc gctgccgggt ggctggcctt cctgacgacg tccctggcca gcgccctggc
      421 gctttacgcg gcgggcacgg tccttgtgtc gggacccggg gaggccctga ccctctgggc
      481 cttgccgcaa tacggctcgg ccctgcggat cgccgtggat ggcctgagcg ccgtcttcgt
      541 ctcgctgatc gccgtcatct ccctcctggc agccctctac tccattcgct acatggagca
      601 ttaccccgac tacggtgcgg cccggtacta tccctacttc ctgctgttcg tggccgggat
      661 gtacggcatc gtctgcctga cggacctcat gctgttcttc ttcctgttct ggcaactgat
      721 gacgctcacc tcctacgccc tggtccggta tgagtaccgg aagccagaga acgtgcgggc
      781 agcctacagg tacctgctca tgatgcagat cgcctgcggg ctcgtcatgc tgggcgcggg
      841 cctgctgggt ctgaaccccg tggtcctccg cggcagcgag cgcctgatga cgtatgactt
      901 cgacgccatc gctcacggtg tgcccgggct cctccagcag ggaggcgggg ccgtggccct
      961 ggccttcctc tgcttcctcc tgggcttcgg catcaaggcc ggcatgtggc ccttcggcca
     1021 gatgtggctg cccgatgccc accccgcggc cccctcgccg gtgagcgccc tgctctcggg
     1081 cgtgatgatc aagacgggca tctatggcct catgcgcgcc ttcctgtggc tgatcccgcc
     1141 cgccgcggca gccgagtatc ccagcggggg ctggggcctg ctcatcgcgg ggctcgggac
     1201 gatcacgctg ttcgtcggga ccatgcaggc catgcggcag gagcaatcca agcggctgct
     1261 ggccttccat agcatcgggc agatcgggta catcctgctg ggcctcggga cgtgcttggc
     1321 ccttctgccc gcaggcctga agagcgaggc cgtgctggcc ctggccacgc tggggttcta
     1381 cggggcccta tttcacaccg tgaaccatgg gatcttcaag agtctgttgt tcctgaacgc
     1441 aggctccatc ctgaccgcga ccgagacaca ggacctcaac aagctcggcg ggttgatgaa
     1501 gttcatgccc gccaccgccg tcatgacgct tatcgcctcc tgctccatcg ccggcgtccc
     1561 cctgttcaat ggcttcgcca gcaagtggag tctgtacgtg gcggccattc tggggagcgg
     1621 ccacgccggg tatctcgccg tgtgcgccgt catcgcgatc ttcaccagcg ccatcaccct
     1681 ggcgtctttc ttgaagttct tcggtgggag cttcctctcg cgcacgagtt cgctggtggc
     1741 cggtcgggcg atcagccggg ggcgtctgga ggtcggctgg ctgatgcaaa tcccgcaggt
     1801 cgtgctcgcc gccctctgcc tgatcctcgg catcctgccg ctctcggcct tctggctgtt
     1861 tcagaccgcc atggaccgaa gccagcaagg cctgggaacc ctcctggcca aggctccgct
     1921 ggcgaccgtc tccgggctga ccggcatcga aggcgtcggg ggccgcgcgg ttttcgcgcc
     1981 cctggcgttg ctgctgatct ttggcgggac gctgctgctg gccgtatggg tgtcgcggct
     2041 gggcgaggca aagcgacgtg tcgccgagcc ttggctctgc gggtatatcc aagaaaacga
     2101 gcagaaccgg tacggtgccc acaacctcta tcgcgaaatc acgcgccact tccgctggct
     2161 gggcggcatg cccaggccgc ggcaccagat gggcagctcg acgggctcga catctcggaa
     2221 cgcttaggga agatccctgg gccttcgcac agaaggtggg tgccatacga tggccgcaca
     2281 gaagatcctg aaccagttga aggagcagtt cgccccggcg attcaacgcg cggacgtgcc
     2341 cgcggacaat cggctgttcc tgtacgtcca gccggaggcg atgaagggcg tctgcgagta
     2401 cgtattccgg acgctggacg cccgctacgt cgtgggcatc ggggtggacg atcgccccta
     2461 ttccggagag ttcctggtgg gccacaattt cgccttcgac tcggcccacg tcctgagcag
     2521 tatcctgacg tgcctgccgg gtgaccggcc agagctccag agcatctccg atgtcgtgcc
     2581 cccggccaac tggtcggagc gggagttccg ggacttggtg ggcatcgtac ccgtggggca
     2641 ccccagcccc aagcggctga tcctccccga tggctggcca gagggtgccc acccgttgcg
     2701 caaggacgtt ccctggaact tcgtcccccc gggctacgac gagaatcagt ccttcgcctt
     2761 cgacgatccg cccgagggct gcgtggtcgt gcccgtcggg cccttccata cgacgttgga
     2821 cgagccggcc cacttccggc tgttcgtgga tggagagacc gtccggggct gcgagtaccg
     2881 gggcttcatg gtccaccggg ccatcgagaa gctgggggat tcggtcctta cgtacaacga
     2941 gatccccatg gccgcggagc gcatctgcgg catctgcggc tgtgtgcaca acgtggccta
     3001 tgcccaggcg gtcgagcaag cggccgcgat cacgccgtcc ccccgggcgg agtacgtccg
     3061 gaccatcatg ctggaggtcg agcggctgca cagccacctg ctctgggtcg ggctggcgtg
     3121 tcatatcctc ggcttcgaca ccctgttcat gcagagcttc cgcatccgtg agccgatcat
     3181 gtggatcgcc gagaagatca gcggcaaccg gaagacgtat gcgctctgcg tgatcggcgg
     3241 cgtgcgctgg gacatcaccc ccgagctgaa gggggagctg cggcgggtcc tggacaccct
     3301 ggagaaggaa tggatcgcgg tggcggacgc cgtgcggggc gatcgggacg tgcagaagcg
     3361 gacccggggc gtgggcgtgg ccgacaaggc cctggtgaag aacgcggggc tgatcggccc
     3421 catcgcccgg gcctcgggcg tggggatcga tgtgcgccgc gaccaccctt acgcggccta
     3481 cgaccgcgtc gacttcgacg tggtggttca ggacggcggg gacgtctggt cacgcatcct
     3541 ggtgcgcgtg ctggaggtgt tcgaatccat caagatcatc cgccagtgcc tggagaagat
     3601 gcccgaggga cccctccaga ccgaggtccg ggacgacctg ccggtgggtc ggctcggcct
     3661 ctcctcggtc gaggcgcccc ggggcgaatc ccaccacttc gtgatcaccg gggaagacaa
     3721 ccggccgcgg cgctggcggg tgcgggcccc cacgtaccag aacctgcagg gggtcccggc
     3781 catgatcaag gaccagcagc tcgccgacat gacgatctct ctgggaagca tcgacccgtg
     3841 cttctcctgc accgatcggc tggagatcct ggacatccgc tccggggcgc aacgcgtcta
     3901 ctcgcaggag gagctcctgc agctcacccg gaaaggcagg ccccaggaat gatgggacag
     3961 agagggatgg aaacccatgg gtagcggcct ggttctctcg ctcgcgaccg gcgtcgccaa
     4021 cgtggcgctg gtgctcctgg tggccccgtt cttcgagggc gtcctgcgga aggtgacggc
     4081 caaggtccag tcgcgtcagg gcccgcccct gtggcagccc tactacgatc tcctgaagct
     4141 tctggggaag gaggacatcg agtcgggtga ggtccccggg atgcagcggg ccgccgccta
     4201 tctctccttc gcggccattc tgaccgtggc caccctggtg cccatgggcg cgccggcccc
     4261 cctgcgtggg gcgggggacg ccgtcttgct catttatctc ctgaccctgt gcggcatctc
     4321 cacgctgctc gcgggcctgg ccgccggctc cacctactcc ttgctgggca tgagtcgcga
     4381 gatgatggcc atgatgaccc tggagccgct cctggccgtg gccctggtca tcggcgccgt
     4441 tcacaccggc tcgctgcgcc tggaggttgt cctcgacgga tcggcctaca cctccgggac
     4501 catcccctgg gccggcatga tcatggcggg cgtcctgctg ctctcgctcc aggcccacct
     4561 gggccgggtc cccttcgaca tctgcgaggc cgagacggag atcatggaag gacccatggc
     4621 ggagtactcg ggccccaagc tggccctgtt caagtacgtg cggatggcaa agctcgtcgt
     4681 ctacagcgcg ctcttcgtgg ccctgttcgt cccctgggga agccacgggc cgtatccagt
     4741 gaacctgctg gtgctcctgg tcaagatcct ggggctggtg ctgttcgtga cgctcgtggc
     4801 ggcgacccat gcgcgctacc ggatcgacca ggccatccgg tattacgccg ggctgttcgc
     4861 ggcctccatg ctggccctgg gactcgcggt ctccgggttc tgaacaatag ggggatgcgg
     4921 atgttcctgc tgagcaagtt caaggaagcg gcgatctgcc tcaaggccgg ccgggtgacc
     4981 ctcccctatc ccgtggagcc gcggccggtg cccaagggct tccggggtcg gcccatcttc
     5041 gacgccgcca agtgcatcgg ctgcgccggc tgcgccaata actgccccgc ccgcgagatc
     5101 ctcgtcctgg acgtctgcca ggagatccgg atcatcaagt accttgggcg tcgctgcacc
     5161 tactgcggcc gctgcgcgga tgtctgcccg gagaaggcga tcagcatgag cccggagttc
     5221 gagaccgcca ccaacgacat ccacgacatc tcgcaggaac tgtaattgtt catggggact
     5281 tgccagcggt gcggccgctg cttcaaggag cccgggccgc tggagcagtt gaagatgaag
     5341 ggctaccggt tcgacctgtt gaaggaggac cggtgggtgt atcggtcgaa ggcctacctg
     5401 gacgatcaac ccgtcgtccg ggatatcgac atcgagttgg attagccccg agacagggag
     5461 cgtggcatgc tgaaggagct gtgtcagaag gtctttcccc gatcgctgtg ggtctatcac
     5521 gccaacagcg gcgggtgcaa cggatgcgac atcgaggtcc tcaacgtgct cacgccgtac
     5581 tacgacgtcg agcgcttcgg catcaagctg gtgggatccc cccgccatgc ggacgtcatg
     5641 ctgtgccaag ggccggccat gcgctcgacg gccaaggcgc tccggcgggc ctacgaggcc
     5701 atgccgcagc cgaagctcgt cttcgccatc ggctcctgcg cctgcggggg cggcatctgg
     5761 cacgacacct acagcgtcct cggccccgtg gaaaaggtgc tccccgtgaa cttctacatc
     5821 cccggct
//
LOCUS       DBNGZC010000875         3663 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2130100, whole genome shotgun sequence.
ACCESSION   DBNGZC010000875 DBNGZC010000000
VERSION     DBNGZC010000875.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3663)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2130100"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1362)
                     /locus_tag="AC16G0_15180"
     CDS             complement(4..1362)
                     /locus_tag="AC16G0_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_334096842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUAEP/CCAEP-tail radical SAM (seleno)protein"
                     /protein_id="HIR5100997.1"
                     /translation="MRVVLVSTYELGRQPFGLASPAAWLRQAGATVRCLDTAVAPLDE
                     QAICDADLVAFYVPMHTATRLAAALVPRITRINPHAHLCFYGLYAPVNEAYLRGLGAN
                     TILGGEFEAGLTGLVRRLSAGEDGGQVEPVISLDRQQFLVPDRHLLPPLSWYAQLNLG
                     DGCLRTVGYTEASRGCMHLCRHCPVVPVYAGRFRIVQRDVVLEDIRRQVAAGAEHITF
                     GDPDFFNGVGHALALVQALHAEHPKLTYDVTSKIEHLLRHRRHLPTLRDTGCLFVTSA
                     VESVDDRVLALLDKGHTRKDFEEVVRFFRGIGLNLNPTFVAFTPWISPQGYLDLLEAL
                     ADLDLVDHVAPIQLALRLLIPAGSKLLDLPEVQSLVEGFDPVGLSYSWRHPDARVDRL
                     QTEIQRLVEQAEADGSSRQEIFGRVWRSAAQAAGGVSRSAPQPRGPARTPIPYMNEPW
                     YC"
     gene            complement(1417..1737)
                     /locus_tag="AC16G0_15185"
     CDS             complement(1417..1737)
                     /locus_tag="AC16G0_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HIR5100998.1"
                     /translation="MAPRMVQCVKLCRELPGIDESTNEGEAAAVFLRSLGSEDLAKRV
                     LEKVSMDAWRMWMGHLTMVINEYRLDPASDEANEIIRDQLEQFFFGEGAALPPGYVPP
                     QSKM"
     gene            complement(2087..2716)
                     /locus_tag="AC16G0_15190"
     CDS             complement(2087..2716)
                     /locus_tag="AC16G0_15190"
                     /inference="COORDINATES: protein motif:HMM:NF033779.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0032543 - mitochondrial translation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44 domain-containing protein"
                     /protein_id="HIR5100999.1"
                     /translation="MFDLLLLAGIGYLIFWMVRRNRMSQEAPAAGYYGRTSAESVGAA
                     VAPGATLDQPPPFGAEDRELGLAHIRQMDPRFDEGAFTEWCTDTFFRIQAAWMRRDVD
                     ALKPVLTEEMQESFRKLIEDAKAKGQTNKLENITVRSVQITEVWQEQGLDYLTVRFLA
                     NNLDYTVEDATGKVVSGSATEPVKFEEYWTWVRPVGPNPWRLSAINQAD"
     gene            complement(3316..>3663)
                     /locus_tag="AC16G0_15195"
     CDS             complement(3316..>3663)
                     /locus_tag="AC16G0_15195"
                     /inference="COORDINATES: protein motif:HMM:NF013722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HIR5101000.1"
                     /translation="VHYEPRFAQEKGFSGIVCHGLLTGSMLCEMGGQIAWLATGMSFR
                     FRRPVYPGDTIACRLVISEIDERGRARAAVGFTNQHGEQVATAELTGYLPTGRACDIL
                     ADMLAEGDPTNPI"
BASE COUNT          650 a         1198 c         1177 g          638 t
ORIGIN      
        1 agctcagcag taccacggtt cgttcatgta aggaatcggc gtgcgagccg gtcctcgggg
       61 ctggggggcg gatcgggaca cgccaccggc cgcctgtgcg gcggaccgcc agacccgccc
      121 gaagatctct tgccgactcg acccgtccgc ctccgcctgc tccaccaagc gctggatttc
      181 cgtctgcagc cggtccaccc gcgcgtcggg atggcgccaa ctgtagctca ggccgaccgg
      241 gtcgaagccc tccaccagtg actgcacctc ggggaggtcc agcagcttgg agccagccgg
      301 gatcaacagc cgcagggcca gttggatagg cgcgacgtgg tccacgaggt ccagatccgc
      361 cagcgcctca agcaagtcca ggtagccctg cggtgagatc cacggggtga atgccacgaa
      421 cgtgggattc aagttcagcc caatgccgcg gaagaagcgg acgacctcct cgaagtcctt
      481 gcgggtatga cccttgtcca ggagagccag aacccggtcg tccacggact ccaccgcgct
      541 ggtcacgaac aggcagcccg tgtcgcgaag cgtcgggagg tgccgccggt gccgaagcag
      601 gtgctcgatc ttgctggtga catcgtaggt cagcttcggg tgttcggcgt ggagagcttg
      661 caccagcgcc agggcatgac cgacgccgtt gaaaaagtcc ggatccccga aggtgatgtg
      721 ctccgccccc gccgccacct gccgccggat atcttccagg accacgtcgc gctggacgat
      781 gcgaaagcgc cccgcgtaga ccgggacaac cgggcaatgg cggcagaggt gcatacagcc
      841 ccggctggcc tcggtgtagc cgaccgtgcg gaggcagccg tcgccgagat tgagttgtgc
      901 gtaccacgag aggggcggaa gcagatgtcg gtccggaacc aggaactgtt ggcggtcgag
      961 ggaaatcacc ggctcgacct ggccgccgtc ttcgcccgcg gagagtcgcc gcaccagacc
     1021 ggtcaggccc gcctcgaact ccccgccgag aatcgtgttg gccccaagcc ctcgcaagta
     1081 tgcctcgttc acgggtgcgt acaggccgta gaagcagagg tgggcgtggg gattgatgcg
     1141 tgtgatgcgg ggaacgaggg cggcggcgag acgggtcgcg gtgtgcatgg ggacatagaa
     1201 tgccaccagg tcggcatcgc aaatcgcctg ctcgtccagg ggcgccaccg cagtgtccag
     1261 gcatctgacc gtggcgcccg cctgacgcag ccaggccgcg ggcgaggcca gcccgaacgg
     1321 ctgccgaccg agctcgtagg tggagaccag gacgaccctc agcccgtact tctccacgga
     1381 tcgatccccg gcttcagccc cgctacccgt gggggctcac atcttggact ggggcgggac
     1441 gtaaccggga ggcagcgcgg ccccttcccc gaagaagaac tgctcgagtt ggtctcggat
     1501 gatctcgttc gcctcgtccg acgccgggtc gaggcgatac tcgttgatca ccatggtgag
     1561 atgccccatc cacatccgcc aggcatccat ggagaccttc tccaggacac gcttggccag
     1621 atcctcggag ccgagcgacc ggaggaagac cgcagccgcc tccccctcgt tggtgctctc
     1681 gtctattccg ggcaattccc ggcacagctt gacacattgc accatccgcg gcgccattcg
     1741 gattcctccg cccgggcacg ccctgccgct cgctcgcggc gggtacccgt ctctggtggg
     1801 tcggtttgcc agcctcttca ctcgcgggat tcagcaggat gccaaggagg atgatactcg
     1861 cctaacaccg gcctgtcgaa agatttacat gaatgggagc agctaggaac cgggcatggg
     1921 aggctgggga acacctccag caggcggacc aggtcgatgt tgggggaggc aggcacacca
     1981 tccggccgaa gctacccttg gcaataaggt ggagaggtga atggctgcgg gtccgaggcg
     2041 catccccagc agccagtcga aaggggtggg tatcccttga atcccgtcag tccgcctgat
     2101 tgatggcgga gagtcgccaa gggttcggtc ccacgggccg gacccaggtc cagtactcct
     2161 cgaattttac cggctcggtg gcgcttccgc tcacgacctt ccccgtcgcg tcctccacgg
     2221 tgtaatccag attgtttgcc aggaagcgga cggtcaggta atccagcccc tgctcctgcc
     2281 agacttcggt gatctgcacc gagcgtacgg tgatgttttc cagcttgttg gtctgcccct
     2341 tggccttggc gtcctcgatc agcttccgga agctttcttg catctcctcg gtcaagaccg
     2401 gcttgagcgc gtccacgtct cgtcgcatcc atgcagcctg gatccggaag aaggtgtccg
     2461 tgcaccactc cgtgaacgca ccctcgtcga accgtggatc catctggcgg atgtgggcga
     2521 ggcccagctc tcgatcctcg gcaccgaagg ggggcggctg gtccagtgtg gctcctggag
     2581 cgacggccgc cccgacggat tcggcggacg tccggccgta atacccggcg gccggggcct
     2641 cttgcgacat ccggttccgc cggaccatcc agaagatcaa gtacccgatg cctgccagga
     2701 gcaagaggtc gaacattccg aatccacttc cagtggcgcc ctcacctcct gcgaagccca
     2761 gactccgaaa cagcatgcct cccaggaagc cacccagaac accacccgcc agggagcgga
     2821 agaagcctcc tgcagcagac ggctgcccta gcggggtcgg agtcggagtc ggttgggtga
     2881 gaggctgggt gctgggactc gttggtcgcg agggggcgct gtagctccgg ctggggctgg
     2941 agtaacttcg cgatccccga cttcctgagg atgtgccgcc gcccgcccgt gcccagagct
     3001 ccacaggcat ggccacggca aggacggtgg ccacggccag aatcgcgccc gtcctcttct
     3061 ttacgtgtgc gttcatcaat tcctctcctt cttgtgcctg ccttcacttt acatccaacc
     3121 atccaacctg gcatattatg cgggagccga gaacccttgg caagctcggt ttcatgtcgg
     3181 ttggtgggag cgagcggata tcgaagagaa atcgcggatg gcctggatcc cgacccccac
     3241 tggaggcact gcgcgagacc gttcgacccg cctctcggtc cctgtccgca atggacatgc
     3301 cgcaccattt gaatgtcaga tgggattggt cggatcccct tcggccagca tgtcggcaag
     3361 gatgtcgcag gcccgtccgg tagggagata tccggtcaat tccgcggtcg caacctgctc
     3421 gccgtgctgg ttggtgaagc cgactgcggc tctggccctt ccccgctcgt cgatctcgct
     3481 gatgaccagt cggcatgcaa tcgtatctcc cgggtacacg ggacggcgga agcggaagct
     3541 cataccggtc gcaagccagg caatttgccc gcccatttcg cagagcatgc tgcccgtcag
     3601 aagcccgtgg cagacgatcc cggagaagcc cttctcctgg gcgaatcgag gctcgtagtg
     3661 cac
//
LOCUS       DBNGZC010000876         3028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2328011, whole genome shotgun sequence.
ACCESSION   DBNGZC010000876 DBNGZC010000000
VERSION     DBNGZC010000876.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2328011"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..309
                     /locus_tag="AC16G0_15200"
     CDS             115..309
                     /locus_tag="AC16G0_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101001.1"
                     /translation="MATRRIDMLEGGRPIVSHIFHGKDAREAQHNEQSHRKADRSLDA
                     AMRGKRYKGVRITVRRSRRG"
     gene            302..2134
                     /locus_tag="AC16G0_15205"
     CDS             302..2134
                     /locus_tag="AC16G0_15205"
                     /inference="COORDINATES: protein motif:HMM:NF027825.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HIR5101002.1"
                     /translation="MAETPITDQVFTEPDANGEDGYSVAKAEELSEWVFGLQSHAADG
                     RKKATRENHWERDWKFIGGDQWEGPLPSYRRPVTMNAWRRALHVELAVITGGRPTLKL
                     VPQGGVPESDLKVWQDALWALLKKEHLFEQHYPDALMWAWVGDGGWFKVGYGQQGTND
                     LPDVQIRAPHPNKIYPDPDSCDVTLTECAYVCFKDVLDLHTISTRYPEQGWRVKPDGM
                     VSIKWPSDRPTWASAGNETLAPAGGWKMSGDYKRARATVVECWIDDPSRETFSVPQIV
                     NLEAVVQSWLDRLLGANGWPAQAMPTQQEGLQRLATLSRSPTMARYEATKPWFAERLG
                     GLPPQLVAPEVREMPRWRWKYPYGRLITVAHDVVLRDIPNPYGEAFGWEQRWPFVFVP
                     GAHQPSTLWRSGLLSNKAETQRAINKGLSLLLENFVKVTTALVVADDNAMEDEDWDLL
                     TLVPGAKIRKRQSTEFKVVFPEPLPAHAFQYPDYMIRKLEEEVGIHDPPVAPGQSVAA
                     KTVAFLQQKGSFLLGIMAKMGDESLERLGSRVMALMRDRYLPGRPIAYFEGEKMGETR
                     PLPELPASFHFRVEATSAFQELMGSTMAMAQAGEQAKRQSRGTK"
     gene            2131..2607
                     /locus_tag="AC16G0_15210"
     CDS             2131..2607
                     /locus_tag="AC16G0_15210"
                     /inference="COORDINATES: protein motif:HMM:NF025881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HIR5101003.1"
                     /translation="MITAEYAAGFFDGEGCVSLWKDSKEYLTLSVAVANNDRDVLIAF
                     QLQFGGAVRRQRRHQRLNQKVGYRWEIRGRQARPFLELIRPIVRVKKAAVELGLEFLD
                     FRAGNRLTRCVLTSVPDETIPWRRILVRTQETVEREAGFKAQMNALNFRGLSERLN"
     gene            2608..2820
                     /locus_tag="AC16G0_15215"
     CDS             2608..2820
                     /locus_tag="AC16G0_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101004.1"
                     /translation="MPAESIGAEVSQLMRRGPSRGPMKGRRMGQRQAVAVALDEARRG
                     AFGRTAKKKATRKPMLRRTLRRIKRV"
     gene            2825..>3028
                     /locus_tag="AC16G0_15220"
     CDS             2825..>3028
                     /locus_tag="AC16G0_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101005.1"
                     /translation="MPGPTLPVTFPFQLSSVDPDGATESCRFLDDRLREIHYQIALLL
                     GLSMDTPITGPVPLSASMLGSTNQ"
BASE COUNT          585 a          917 c         1032 g          494 t
ORIGIN      
        1 caatgccgat tgccggtgcc ccttgccgaa catgcagggg gcgcactacg tgaacatcgt
       61 ggtgcggcgg aacggacgcg atgaagtcta tgaagccgac gcgctgaggg tctgatggca
      121 actcgacgca tcgacatgct cgaaggcggt cgccccatcg tgtcccacat cttccacggg
      181 aaggacgccc gcgaggccca gcacaacgag cagagccacc gcaaggccga ccgctcgctg
      241 gacgccgcga tgcggggcaa gcgctacaag ggggttcgca tcaccgtgag acggagtcgc
      301 cgtggctgaa acgccgatca ccgaccaagt ctttacggag cccgacgcca acggcgagga
      361 cggctacagt gtcgccaagg ccgaggaact gagcgagtgg gtcttcgggc tccagtcgca
      421 cgcggcggat ggccggaaga aggccacacg cgagaaccac tgggagcgcg actggaagtt
      481 cattggcggc gaccagtggg aaggcccgct cccctcctac cgccggccgg taacgatgaa
      541 cgcctggcgg cgggcgctgc acgtcgagtt ggcggtcatc acgggcggtc ggcccacgct
      601 caagctcgtc ccgcagggcg gcgtccccga gagtgacctc aaggtttggc aggacgcgct
      661 gtgggcgctc ctcaagaagg agcacctctt cgagcagcac taccccgacg cgctcatgtg
      721 ggcgtgggtc ggggacggcg gctggttcaa ggtggggtat gggcagcagg ggacaaacga
      781 cctccccgat gtgcagatcc gcgctccaca ccccaacaag atttaccccg acccggactc
      841 gtgcgacgtg acgctgaccg agtgcgccta tgtctgtttc aaggacgtgc tcgacctgca
      901 caccatcagc acgcgctatc ccgagcaggg ctggcgggtg aagcccgacg gcatggtgtc
      961 catcaagtgg ccgagtgacc ggccgacctg ggcgtcggcg ggtaacgaga cgctggctcc
     1021 ggcaggcgga tggaagatgt ccggggacta caagcgggcg cgggccacgg tggtggaatg
     1081 ctggattgac gacccctccc gcgagacgtt cagcgtgccg cagatcgtga acctcgaagc
     1141 ggtggtgcag tcctggctcg accggctcct gggggccaac ggctggcccg cgcaggcgat
     1201 gccgacgcag caggaagggc tccagcggct cgcgaccctg agccgctcgc cgacgatggc
     1261 ccgctatgag gcgaccaagc cgtggttcgc cgagcggctc ggcggattgc cgccgcagtt
     1321 ggtggctccc gaggtgcgcg agatgccgcg ctggcggtgg aagtacccct acggccggct
     1381 catcaccgtg gcgcacgacg tggtgctgcg cgacatcccg aacccgtatg gcgaagcctt
     1441 cgggtgggag cagcgatggc cgtttgtgtt cgtgccgggg gcgcaccagc cgtccacgct
     1501 ctggcgctcg ggactcttga gcaacaaggc ggagacccag cgggcgatca acaaggggct
     1561 gtccctcctg ctggaaaact tcgtcaaggt cacgacggcg ctggtggtcg ccgacgacaa
     1621 cgcgatggaa gacgaggact gggacctcct gaccctcgtg ccgggggcga agatccgcaa
     1681 gcgtcagtcc acggagttca aggtggtgtt ccccgaaccg ctgccggccc acgccttcca
     1741 gtacccggac tacatgatcc gcaaactgga ggaggaggtg ggcatccacg acccgcccgt
     1801 ggcccccggt cagtcggtgg cggcgaagac cgtcgccttc ctccagcaga aggggtcgtt
     1861 cctgctcggc atcatggcca agatggggga cgagtcactg gagcgtctcg gctcgcgggt
     1921 gatggcgctg atgcgggacc gctacttgcc gggccgacct atcgcttatt tcgaaggtga
     1981 gaagatgggg gagacgagac cactgccaga actcccagca tccttccatt tcagagtcga
     2041 agcaacctcc gcgttccaag aactcatggg ttcgaccatg gcaatggctc aagcgggtga
     2101 acaggcaaag agacaatcgc gggggaccaa gtgattaccg ccgaatacgc cgcagggttc
     2161 tttgacggag aaggttgcgt ctcgctgtgg aaggactcga aggagtacct taccctctct
     2221 gtcgctgtgg ccaacaatga ccgagatgtt ctgattgcct tccagttaca gtttgggggt
     2281 gccgttagac ggcaaaggag acaccagagg ttaaaccaga aggttggcta tcggtgggag
     2341 attcgtggtc gtcaagcaag gccattcctt gaactcatac gcccgatagt gcgagttaaa
     2401 aaggctgccg tagaactcgg ccttgagttt ctggattttc gtgctgggaa taggctaact
     2461 cgctgtgtgc tgacatcggt gccagacgag accattccat ggaggcgaat ccttgtccga
     2521 acccaagaga ccgtagagag agaagccggg ttcaaggcgc agatgaacgc cctgaacttt
     2581 cgaggtctct cagaaaggct caattaaatg ccagccgaga gtatcggggc tgaggtgtcc
     2641 cagttgatgc gacgtgggcc gagtcgcggc ccgatgaagg ggcggcggat ggggcagcgg
     2701 caggcggtcg cggtggccct ggacgaagcg cggcgcggag ccttcgggcg cacggccaag
     2761 aagaaggcca cgcgcaagcc gatgctgcgg cgcaccctga gacgcatcaa gcgggtgtaa
     2821 gccgatgccc ggcccgaccc tgcccgtcac gttcccgttt caattgtcct cggtcgatcc
     2881 agacggggcc acggagtcct gccgttttct cgatgaccgc ctccgcgaga ttcactatca
     2941 gattgccctg ctgctcgggt tgtccatgga cacgccgatc acggggccgg tgccgctgtc
     3001 ggcgagtatg ctgggctcga ccaaccag
//
LOCUS       DBNGZC010000877         2064 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2227198, whole genome shotgun sequence.
ACCESSION   DBNGZC010000877 DBNGZC010000000
VERSION     DBNGZC010000877.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2227198"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="AC16G0_15225"
     CDS             complement(<1..532)
                     /locus_tag="AC16G0_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101006.1"
                     /translation="MSAYRRETIVWSLLTLGAFAVLPWERVGKAFLAFSWPQTGLGQT
                     ATHWSLAPVGLAAGLAVLLALGGKDNRGIGRLILALSVLGLAAALYQLSTAGRAFGLG
                     AMVSLLGLLTLAGIGVAQAGYLRGGSFVGAGILWSAGLVTIFILFPLCMMLQASVVVQ
                     GHLTAAGLWRYLRSPIF"
     gene            complement(639..1658)
                     /locus_tag="AC16G0_15230"
     CDS             complement(639..1658)
                     /locus_tag="AC16G0_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5101007.1"
                     /translation="MRMRGSLTLLAIGLLLLFAVPALAGESKVVVLCSPDPAWCEAVK
                     REFSKTGTQVDFVRLSSGAGLARLRAEKANPTFDAWFGGSGDGYWVAQAEGLIEFYQP
                     KAWPELRPQLTKAVDGHYIPLYTGIHGFGINEKILKEKNLPVPQTWKELGDPKYKGLI
                     AMPNPNTSGTGYVMMTTIIQVYGEAPGFELLKAIHRNVAQYTRSGGDPSLLAGRGEAA
                     IGVSFANDVVERARKGFPITFIAPKDGTGYEIGGIGLVKGAPNRANGMKLIDWALEPE
                     NQKLGPQNGEVSIPSHSKSAVDPDVPRFESVNAINYDFGKYGTPEVRDGLIRKWTEQV
                     FPLPK"
BASE COUNT          344 a          704 c          686 g          330 t
ORIGIN      
        1 ggaagatcgg cgatcgcaga taccgccaga gccctgcggc agtgaggtgg ccctggacca
       61 ccacgctggc ctggagcatc atgcagagcg gaaacaggat aaagatcgtc accaggccgg
      121 ccgaccacag gatgccggca ccgacgaaac tgcctccccg gaggtagccg gcctgggcca
      181 ctccgatccc ggccagcgtc aggagaccca agagactgac catggccccc aggccgaagg
      241 cgcggccagc cgtggagagc tggtacaggg ccgcggccag accgaggacc gagagcgcaa
      301 ggatcaatcg cccaatgccc cggttgtcct tccccccaag cgcgagcagc accgccaatc
      361 ctgcggcgag acccacgggg gccaagctcc agtgggtcgc cgtctgtccc aggccggtct
      421 ggggccagga aaaggcgagg aaggccttcc cgacacgctc ccacggcagg acggcgaacg
      481 cccccagggt gagcaggctc cacacgatgg tctcgcggcg ataggcactc attctacgaa
      541 ccggggaatc ggggaaccgg cgagccggcg acacaagcaa ggacattgca cggtcacgcc
      601 ctccccgatt ctccgattct cccaatcacc gattcctgtc atttcggcaa tgggaatact
      661 tgctctgtcc acttcctgat caggccgtcc cggacctccg gcgttccgta cttgccgaag
      721 tcatagttga tggcgttcac gctctcgaag cgcggcacgt cgggatcgac agccgatttc
      781 gagtgggacg ggatgctcac ctcgccgttc tggggaccca gcttctgatt ctccggttcc
      841 agggcccagt cgatgagctt catgccgttc gcccggttgg gagccccctt caccaggccg
      901 atgccgccga tctcgtaccc ggtcccgtcc ttgggcgcga tgaaggtgat ggggaaacct
      961 ttcctcgccc gttccaccac gtcgttggcg aaggagacgc cgatggcggc ctccccccgg
     1021 ccggcgagga ggctgggatc gccgccgctc cgggtgtact gcgccacgtt ccggtggatc
     1081 gccttgagca gctcgaagcc gggagcctcg ccgtagacct ggatgatggt ggtcatcatc
     1141 acgtacccgg tccccgaggt gttggggttg ggcatggcga tgaggccctt gtacttcgga
     1201 tcgcccagct ccttccaggt ctgcgggacc ggcagattct tctccttcaa aatcttctcg
     1261 ttgatgccga agccgtggat cccggtgtag agcgggatgt aatgtccgtc cacggccttg
     1321 gtgagctgcg gtcgcagctc gggccaggcc ttgggctggt agaactcgat gaggccctcc
     1381 gcctgggcga cccagtagcc gtcgccgctg ccgccgaacc acgcatcgaa ggtcgggttc
     1441 gccttctcgg cgcggaggcg ggccagtccg gcccccgagc tcaggcggac gaaatccacc
     1501 tgggtgccgg tcttgctgaa ctcccgcttc accgcctcgc accaggcggg atcggggctg
     1561 cagaggacca ccaccttgct ctcgcccgcc agggccggca cggcgaagag gagcagcagg
     1621 ccgatcgcga gcagcgtcaa cgagccacgc atcctcatga cgacgcctcc ttcttggtcc
     1681 gtccgcccca gggggcgacg gggattcgcg gcccggcgtg ggcggcgccc tacgtccggg
     1741 cctggtgcgg gacggcgaac acccgctgcg aggcctcgag ggtggccatc ggcagcaggg
     1801 acagatgcgg gaaaggtacg cccggcgggc tgcgcgggtc gccgccacgg gctcaccggg
     1861 gttgtcctga aacctgtggc gttccgcgat cctccgagat gcgcctcgtc gcggcgcgcg
     1921 aacccgtgat caccgggagg tctcggctgt caggccgtca ggatagaaca gaatggcccc
     1981 ctgtgccaag agcgtccttt gcacggtccc gacgtcgacc ttccgcgggg agaggcctgc
     2041 ctggcgcgcc agcgcgtctg ccgt
//
LOCUS       DBNGZC010000878         1498 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_173777, whole genome shotgun sequence.
ACCESSION   DBNGZC010000878 DBNGZC010000000
VERSION     DBNGZC010000878.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1498)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1498)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1498
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_173777"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1357)
                     /locus_tag="AC16G0_15235"
     CDS             complement(131..1357)
                     /locus_tag="AC16G0_15235"
                     /inference="COORDINATES: protein motif:HMM:NF006771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HIR5101008.1"
                     /translation="MIRINLPRLMRDLEGLGAIGRTPGGGVSRPSFSDADMEARRWLL
                     ARIAAAGLEARTDAAGNIFGRWQTGSPVVMVGSHLDSVPNGGRFDGALGVLAGLECLR
                     RIKEEGVRLNHPLELVAFTDEEGTFGGFFGSYAFTGVLKADAIPSISDSKGLGIVDAM
                     ARHGMDATQAPSARRDPKEIRAYMELHIEQGPVLESKKIPIGVVDAIVGIRRFGITFH
                     GRADHAGTTPMTDRKDALLGATDLIQKGREMVLAEGRAASRLTVGVIQVQPGVSNIVP
                     AEAYLTYELREVSSEVLAKLADRSRSLAESVASAQGLTVSIETILEIEPVTLADEVKA
                     AIVAATNELGLSHLRLPAMAGHDAQVLGRVAPAGMIFVPSKDGRSHSPMEYTSDEDVE
                     RGTNVLLQTLLKLAAG"
BASE COUNT          269 a          523 c          431 g          275 t
ORIGIN      
        1 ccccgatcgg gaatcactcg gatgaatcgc ccgaaagacc tcgcacccga tgatttcccg
       61 catcgatgcg agcccagagt ctttgcgatc ttggcgttct tggcggtgaa atcttcgggc
      121 ctggttagga tcagcctgcg gccagcttca gcagggtctg gaggagaacg ttcgtcccgc
      181 gctcgacatc ctcgtccgag gtgtactcca ttggcgagtg gctgcggccg tccttgctgg
      241 ggacaaagat catgccggct ggagcgacac gacccaacac ctgggcatcg tgacccgcca
      301 tggcgggcag gcgaagatgg ctgagcccca gctcgttcgt ggcagctaca atggctgcct
      361 tgacctcgtc ggctaaggtg accggctcga tttccaggat cgtctcgatg gacaccgtga
      421 gcccctgagc cgaggcgacg gactcggcca gactacggga tctgtcggcc agctttgcca
      481 gcacctcgct cgatacctcg cgcagctcgt aggtaagata ggcctcggcc gggacgatgt
      541 tggaaactcc cggctgtact tgaatcacac ccaccgtgag cctgctcgcg gcccttccct
      601 cggcgagaac catctcccgc cccttttgga tcagatcggt cgccccgagg agggcgtctt
      661 tccgatccgt catgggtgtc gtccccgcgt gatcggcccg accgtggaag gtgatgccga
      721 accggcgaat gccgacgatg gcgtccacga cgccgatggg gatcttcttg gattccagca
      781 ccggcccctg ctcgatgtgc agttccatgt aggcccggat ctccttcggg tcgcggcggg
      841 ccgagggagc ctgcgtcgcg tccattccat gccgggccat cgcgtccacg atccctaacc
      901 ccttcgagtc gctgatgctc ggaatagcat ccgctttcag aacccccgtg aaagcatagc
      961 tcccaaagaa gccgccgaag gtcccctcct cgtccgtgaa tgccacaagc tccagcgggt
     1021 gattcagacg gacgccctct tccttgatcc gccgcagaca ttccaggccc gccagcactc
     1081 ccaaggcgcc gtcgaacctg ccgccgttcg ggaccgagtc gaggtgagag cccaccatga
     1141 cgacagggct accggtttgc cagcggccaa agatgttgcc cgcggcatcc gtacgcgcct
     1201 ccaatccggc ggcggcgatc cgcgccagca accagcggcg cgcctccata tccgcatcgc
     1261 tgaaggacgg ccgcgacacc ccgcctccgg gcgtccgacc gatcgccccc aacccctcca
     1321 ggtcccgcat gagcctcggc aaattgatcc ggatcatcac ctcaccccca aatgcaccac
     1381 aatggtcggg gagactaagt accgccgcta cgcctggtat cctaacaccc cccttcgcac
     1441 cgactcaagg aaatacaccc tccttcatcg agtgtgtgag tctctccggt ttgggttg
//
LOCUS       DBNGZC010000879         6888 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_759436, whole genome shotgun sequence.
ACCESSION   DBNGZC010000879 DBNGZC010000000
VERSION     DBNGZC010000879.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6888)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_759436"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="AC16G0_15240"
     CDS             complement(<1..190)
                     /locus_tag="AC16G0_15240"
                     /inference="COORDINATES: protein motif:HMM:NF024313.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5101009.1"
                     /translation="MSSVAPTVDERSQTPAESPMRLLLRRLWRNRLAVTGFALLLLVL
                     LGALLAGVLSSYDPLTMTV"
     gene            complement(183..1127)
                     /locus_tag="AC16G0_15245"
     CDS             complement(183..1127)
                     /locus_tag="AC16G0_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019120362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5101010.1"
                     /translation="MTLYVVRRLVSVVPVLILVAVASFLLVHLVPGDPAMVMLGNDAT
                     PQQIQTLRTQMGLDRSLPEQFVLWVREVLRGNLGESFFLGRPVSQALLERLPATLQLA
                     LLSLFFSLLIGVPAGILAAVRQNTRWDQLIMVTAMGGISIPSFWLGLTLILVFSVQFG
                     WLPSGGYTPLWEDVWQGLRVLILPAISLGFMQAALIARMTRSSMLEVLRQDYVRTAKA
                     KGLNWERVTLRHALKNAMIPVITTIGTAFGVLLGGAVIVEIVFTYPGLGRLVVLAVQR
                     RDYPLVQGALLLTSVIYVLVNLAVDLLYSVFDPRITYE"
     gene            complement(1220..2770)
                     /locus_tag="AC16G0_15250"
     CDS             complement(1220..2770)
                     /locus_tag="AC16G0_15250"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5101011.1"
                     /translation="MYGRLRFWMGMTVCMLVGLLLAVGPAMAGTPMTGGVLRAALTTS
                     APTLDPHSATHLAVREIGLHIFESLLTFDAKFQVVPQLAERWEVSQDGKVYTFHLRKG
                     VKFHNGKEMTAEDVRASVERFRTMAPRRNELDGLARLEVLGPTVVRMILKQPDAAFLA
                     AIANPICQLAILPKEAVEGRPINKADIIGTGPYKFVEWIPDRWVRVARLADYRPEGKE
                     EPAGFGGARHAYADEIRFIPVPEPGARVAGLQTGEYDFADFLPSASIPQLTGDRSLRI
                     VRLGPQNYPTVYFNHTGKFKNLKLRQAVQAALNMDEIMQVANDGDGRVDPGFYFKEQV
                     WHTNAGGKLYNPKNPAKAQQLLEAAGYKGEPVVFVTNSDYDYMYKAAQVIQQQLKKVG
                     MNVKLEVYDWPGSLAVRKDMSKWDLFTSSHSTRLDPSANDFYFRAKTSFLGYNNPKME
                     ALLDKASAATKFEDRYKIYEDVQRLIYEDVPMLKLYDHFTWQGYSAKLQGYTPWVQVR
                     LWNVWREK"
     gene            complement(2847..3698)
                     /locus_tag="AC16G0_15255"
     CDS             complement(2847..3698)
                     /locus_tag="AC16G0_15255"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR5101012.1"
                     /translation="MSGGRCAYRLEELSWPQAQAAIGSGAMAIIPVGSLEQHGRHLAV
                     NTDNVIADGLGEEIARRGSAQGLKLLLCPAIHYGYTMHNMDFPGTITLRMETLLELGV
                     DVVSSLVHHGCRKVVLLNAHGSNWSILDLISRRVMNRHPGALVAAISPIKMAAAELEE
                     LREAKELGGMSHGCELETSLMLHLRPELVEMDKAVRDITQPDSRFYWRDLLRGSRGVA
                     MADLTRHASRTGLVGDPTLADAAKGSRCFEVIVSTCLEFLTEFARREVRVTADFPLTH
                     PRTDIYE"
     gene            complement(3843..5018)
                     /locus_tag="AC16G0_15260"
     CDS             complement(3843..5018)
                     /locus_tag="AC16G0_15260"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR5101013.1"
                     /translation="MHATSASTDAADVREAVRQLLMELIAIPSYGAQEEAVIRHLMQR
                     FSTQGIPCRITELEGKPLNLVAEIGRGPRTILLNSHVDTVPPGERSPWLTDPLTPVEK
                     DGVIYGRGAGDAKGCLAAMIVAFEMLARRGSELPVKAILMAVGAEERGGLGTKLEVEN
                     GVRADAAIIGESTDLVPKMAHKGVLRLEVEVTGRAAHASDPEAGINAVVAMAPLVSAL
                     DRLATGVRQRSEAYTGRASLVISTIAGGVALNVIPANCVISIDRRVLPTETEADATQE
                     IRETVNRALPPASGACVKVRKVRFVPPSFTHPKEAIVAAGERAASAVLGRPIQAGGFT
                     ATCDMTYLVNEGGIPTIILGPGSIDVAHQANEHISIDQMAQAVQVYLRTIATWLTGA"
     gene            complement(5128..6549)
                     /gene="cofE"
                     /locus_tag="AC16G0_15265"
     CDS             complement(5128..6549)
                     /gene="cofE"
                     /locus_tag="AC16G0_15265"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HIR5101014.1"
                     /translation="MSSTSILIPVKSTARAKGRLGSLLDQPTRQQLSLHMLEDVLAAV
                     MPAAGPLVKSVYVATSDPEAMAIARRYGATVLEEQEQRSESYSVDAASRSCAARGVEA
                     LLTIPADVPAIQTEDIAAILREASPERAVVLVPSRDDKGTNAIWRRPPVAIPSRFGYD
                     RFRTHQAEAEARNLAWFALRLPRLAVDIDEPEDLAALLEFPGGTRTRALLEQVGVVGR
                     LPRAEASDLSIVALPGIPEVAEGDDLPRLILEAAERREDGLRTGDVLVVAQKVVSKAE
                     GRVVSLGDVEPSPLARQFAEAWGKDPRSVEVVLRESRRIVRMDRGVIIAETSHGFICA
                     NAGVDASNVPGGERVSLLPVDPDGSAARMAKGLTDVCGAEVAVIVSDTFGRPWREGLM
                     NVAIGVAGLRPLVSYIGQQDPHGHVLRVTELAVADDLAAAAGLLMGKLERIPVVRIRG
                     YRFQAAEGRASVLVRSAERDLFR"
     gene            complement(6528..>6888)
                     /locus_tag="AC16G0_15270"
     CDS             complement(6528..>6888)
                     /locus_tag="AC16G0_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HIR5101015.1"
                     /translation="PILAVPGIRERLRNTKVPLVAVSPVVGGASLKGPTDKFLQWAKV
                     EVSPLGVSRFYQSVLGRLDGMLIDRVDAALTPAMEAEGVRVWVDGIVMRGPEEKRAVA
                     RMACTLLEQMGCRPPRS"
BASE COUNT         1158 a         2421 c         2174 g         1135 t
ORIGIN      
        1 ccacggtcat ggtcagggga tcgtaggatg agagcacccc cgccaagaga gcacccagga
       61 ggaccaggag gagcagggcg aaccccgtga ccgccagccg gttgcgccag agccggcgca
      121 ggagcagtcg catcggcgac tcggccggtg tctgggatcg ctcatcgact gtcggcgcca
      181 cgctactcat acgtgatccg cggatcgaac acagaataca ggaggtccac agccaggttc
      241 accagcacgt agatcaccga ggtgagcagc agggcgccct ggaccagggg ataatcccgc
      301 cgctggaccg ccagcaccac gagcctaccc agccccgggt aggtgaagac gatctccacg
      361 atcactgcgc cgcccaggag cacaccaaag gctgtcccga tggtcgtgat gaccgggatc
      421 atggcattct tgagcgcgtg gcggagcgtg acccgctccc agttcagccc cttggccttg
      481 gctgtccgca cgtaatcctg ccgcaggacc tccagcatgc tggaccgcgt catgcgggcg
      541 atgagggccg cctgcatgaa gcccagggag atggcgggca ggatcagcac tcgcaatcct
      601 tgccagacgt cctcccacag gggggtgtag ccgccgctgg gcagccaccc gaactggacc
      661 gaaaagacca ggatcagggt gaggcccagc cagaagctgg ggatggagat cccgcccatg
      721 gcagtgacca tgatcagttg atcccagcgc gtgttctgcc gcaccgccgc caggattccc
      781 gccgggacgc caatgagcaa ggagaagaac agcgagagca gcgcgagctg cagcgtggcc
      841 ggcagccgct ccaggagagc ctgggagaca gggcgcccca ggaagaagga ctcacccagg
      901 ttcccgcgca ggacttcccg cacccagagc acaaactgct ccggcaggga gcggtccagc
      961 cccatctggg tccgcagggt ctggatctgc tgcggcgtgg catcgtttcc cagcatgacc
     1021 atggccgggt cgccggggac gagatggacc agcaggaacg aggcgaccgc caccagaatc
     1081 agtaccggga cgacgctgac aagccgccgc accacataca gcgtcatggg ctaaccggcc
     1141 cggcccgctg gttcccctcg gtaaggcggc agccactttc caatttcgaa tttcgaaatt
     1201 ccaatttcga aatgccccat cacttctccc gccagacgtt ccagagccgc acctgcaccc
     1261 agggggtgta accctgaagc ttcgccgagt agccctgcca ggtgaagtgg tcgtacagct
     1321 tcagcatggg cacgtcctcg tagatgagcc tctggacgtc ctcgtagatt ttgtaccgat
     1381 cctcgaactt cgtggcggcg ctcgccttgt ccaggagggc ctccatcttc ggattgttgt
     1441 aacccaggaa gctggtcttg gcccggaagt agaagtcgtt ggcgctgggg tctaaccggg
     1501 tggaatgact ggaggtgaat aggtcccact tgctcatgtc ctttcgaacc gccagagacc
     1561 ccggccagtc gtacacctcc agcttgacgt tcatgccgac cttcttcaac tgctgctgaa
     1621 tcacctgggc cgccttgtac atgtagtcgt agtcgctgtt ggtgacaaac accaccggct
     1681 cgcccttgta gccggcggcc tccagaagct gctgggcctt ggcgggattc ttcgggttgt
     1741 acagcttccc gcccgcgttg gtgtgccaga cctgctcctt gaagtagaag ccggggtcca
     1801 cacggccgtc gccgtcgttg gccacctgca tgatctcgtc catgttgagc gccgcctgca
     1861 ccgcctggcg cagcttcaga ttcttgaact tccccgtgtg gttgaagtag accgtcggat
     1921 agttctgcgg ccccagccgg acgatgcgca ggctccggtc cccggtgagc tgcgggatgg
     1981 aggccgaagg caggaaatcg gcaaagtcgt actcgcccgt ctgcagtccg gccacacgcg
     2041 cccccggctc aggcaccggg atgaaccgga tttcatcggc gtaagcgtgc cgcgccccgc
     2101 cgaatcccgc gggctcctct ttgccctcgg gccggtagtc cgccaagcgg gcgacccgca
     2161 cccagcggtc gggtatccac tccacgaact tgtacgggcc cgtcccgatg atgtccgcct
     2221 tgttgatggg ccggccctcc accgcctcct tggggaggat ggccaactgg cagatcgggt
     2281 tggcgatggc agccaggaag gctgcgtcgg gctgcttcag gatcatccgg accacggtcg
     2341 ggcccagcac ctccagcctg gcgaggccgt ccagctcgtt tcgcctgggc gccatggtcc
     2401 ggaaccgctc caccgaggcc cggacgtcct ctgcggtcat ctccttcccg ttgtggaact
     2461 tcacgccctt acggagatgg aaggtgtaga cctttccgtc ctggctgacc tcccaccgct
     2521 ctgccaactg cggcacgacc tggaacttgg cgtcgaaggt gagaagactc tcgaagatgt
     2581 gtaggccgat ctcccgcacc gccaggtggg tggcggagtg cggatccagc gtgggcgcgc
     2641 tggtcgtgag cgccgcgcgc aggacgccgc cggtcattgg cgttcctgcc atcgccggcc
     2701 ccaccgccag cagtagaccg accagcatgc acaccgtcat gcccatccag aagcgaagcc
     2761 ttccgtacat catgctacct cctcttcggt catgcccagc gaccgcaccc tcgcccgtac
     2821 cctggtcgtg cccgcttccc agcccgtcac tcgtagatgt ccgtccgcgg atgggtcaag
     2881 gggaagtccg ccgtgacccg cacctcgcgc cgggcgaact ccgtcaagaa ttccaggcag
     2941 gtggatacga tgacctcgaa gcaccggctg cccttggccg catcggccag cgtgggatcc
     3001 ccgaccagcc ctgtgcggga cgcgtgacgg gtaaggtccg ccatggccac gccgcggctg
     3061 ccgcgcagca aatcgcgcca gtagaaccgg gagtcgggct gggtgatgtc ccgcaccgcc
     3121 ttgtccatct cgaccagctc cggccggagg tggagcatca gcgaggtctc cagctcgcag
     3181 ccgtgggaca tcccgccaag ctccttggcc tcgcgcagct cttccagctc ggcggcggcc
     3241 atcttgatcg ggctgatcgc cgccaccagc gcccccggat ggcggttcat cacccgacgc
     3301 gagatgaggt caaggatgct ccagttgctg ccgtgggcgt tcagcagcac gaccttgcgg
     3361 cagccgtggt gcaccaggct gctcaccaca tccaccccca gctccaggag cgtttccatg
     3421 cgcagcgtga tggtccccgg gaagtccatg ttgtgcatgg tgtagccgta gtgaatcgcc
     3481 gggcacagga gcagcttcag tccctgcgcc gatccgcgcc gcgcgatctc ctcccccagg
     3541 ccgtcggcga tcacgttgtc cgtgttgacc gccaggtgcc ggccgtgctg ctccagactt
     3601 cccaccggga tgatcgccat cgccccgctg ccgatcgccg cctgcgcctg cggccagctc
     3661 agctcctcca gccggtatgc acaccgacct ccgctcatcg cctcatgcct cctctggtcc
     3721 accgaccttc atcagacacc ctgcggacgg gaggcggcag gggcctcccc cttcagcggc
     3781 ctctctcccg cgtggagacg gccggagcga ggggcaccat acgttcaagc gagccctccg
     3841 gactacgccc ccgtgagcca ggtcgcgatc gttctgagat acacctggac ggcctgtgcc
     3901 atctgatcga tggagatgtg ctcgttggcc tgatgggcaa catcgatgga tcccggtccc
     3961 aggatgatcg tcggaatgcc tccctcgttg accaagtagg tcatgtcgca ggtggcggtg
     4021 aatcccccgg cctggatcgg ccgtcccaga acggcagaag ccgcccgctc ccccgccgcc
     4081 acgatggctt ctttggggtg ggtgaaggac gggggcacga accgcacctt ccttaccttc
     4141 acgcatgctc ccgaggccgg gggcaacgct cggttgacgg tctcccggat ctcctgtgtg
     4201 gcgtccgcct cggtctcggt gggcaggacc cggcggtcga tggagatcac gcagttcgcc
     4261 gggatgacat tcagcgccac cccgcccgcg atggtggaga tcaccaggct ggcccttccg
     4321 gtgtaggcct cggagcgttg ccgcaccccg gtcgcaaggc gatccagggc cgagacaagc
     4381 ggcgccatgg cgaccacggc attgatcccc gcctccgggt cgctggcgtg cgccgcccgg
     4441 ccggtcacct ccacctccag cctcaggacg cctttgtggg ccatcttggg cacgagatca
     4501 gtggactccc cgatgatggc ggcatcggcc ctgacgccat tctccacttc gagcttggtc
     4561 cccaggccgc cccgctcctc ggcgcccacg gccatcagga tcgccttcac cggcagttcg
     4621 ctcccccggc gcgccagcat ctcgaaggcc acgatcatcg cagccagaca tcccttggca
     4681 tcccccgctc cccggccgta gatcaccccg tccttctcca cgggcgtcag cgggtcggtg
     4741 agccagggcg accgctcgcc cggcggcacc gtgtccacgt ggctattcag gagaatggtc
     4801 cgcgggcccc ggccgatctc agccaccagg ttcaggggtt tgccctcgag ttccgtgatc
     4861 cggcagggga tgccttgtgt cgagaagcgc tgcatcaggt ggcggatgac ggcctcttcc
     4921 tgggcgccgt aggaggggat ggcaatcaac tccataagca gttgcctgac tgcctcccgt
     4981 acgtcggctg cgtcagtaga agcggatgtg gcatgcatcg ggcgtcacct ctcctctcgg
     5041 gcgtctgcct ggcggtccgt cgaggcacaa tatgcctccc ggcctcacgg tgtcaatgag
     5101 tggaacggca agggtggcag gaacgaacta tcggaagaga tcccgttctg cagagcgcac
     5161 caggacgctg gctcggccct cagccgcctg gaagcgataa ccgcggatgc gcacgaccgg
     5221 gatccgttcc agcttgccca tgagcaggcc ggcggcggcg gccaggtcgt cagccacggc
     5281 cagttccgtc acgcgcaaga cgtgcccgtg cgggtcctgc tggccaatgt agctcaccag
     5341 cgggcggagc ccggcgacgc cgatggcgac gttcatgagt ccctcgcgcc agggacggcc
     5401 gaacgtgtcg gagacgatga ccgccacctc ggccccgcac acatccgtta ggcctttggc
     5461 catccgcgct gccgacccgt ccggatccac cgggaggagc gagacgcgct cccccccggg
     5521 gacgttggag gcatccacgc cggcgttggc gcagatgaag ccgtggctgg tctctgcgat
     5581 gatcacgcca cgatccatcc ggacgatgcg ccgggactcc cgcaggacga cctcgaccga
     5641 ccgggggtcc ttgccccagg cctcggcgaa ttggcgggcg agtggcgagg gctcgacgtc
     5701 cccgagggac accacgcgtc cctccgcctt cgacaccacc ttctgcgcca ccaccaggac
     5761 atcgccggtc cgcaagccgt cctctcgccg ctccgccgcc tcgaggatga ggcggggcag
     5821 gtcgtcccct tcggccacct ccgggattcc cggaagcgcc acaatggaaa ggtcggaggc
     5881 ttccgccctg gggagccggc ccaccacacc cacctgctcc agcagggcgc gcgtcctggt
     5941 gccgccgggg aattccagga gggccgccag atcttcgggc tcgtcgatgt ccaccgccag
     6001 tcggggcagc cggagggcga accaggcgag attgcgggcc tcggcctcgg cctggtgcgt
     6061 gcggaagcgg tcatagccga accgcgacgg aatcgccacc ggcggccgcc gccagatggc
     6121 attggtgccc ttgtcgtccc tcgacggaac caggaccacg gcccgctccg gactggcctc
     6181 acgaaggatg gctgcgatat cctccgtctg aatggcgggg acgtccgccg ggatcgtcaa
     6241 gagggcctcc acgccccgtg cggcgcagct ccgggacgcg gcatccaccg agtagctctc
     6301 ggagcgctgt tcctgttcct ccagcaccgt ggccccgtac cggcgcgcaa tggccatggc
     6361 ttcaggatcc gaggtggcca cgtacaccga cttcaccaag ggtcctgctg caggcatgac
     6421 cgctgcgagg acatcctcca gcatgtggag ggagagctgc tgccgcgtgg gctggtccag
     6481 gagcgatccc agacgaccct tcgcccgcgc cgtggacttg accgggatca ggatcgaggt
     6541 ggacgacatc ccatctgctc caggagcgtg cacgccatcc gggccaccgc ccgcttctcc
     6601 tcggggccgc gcatgacgat cccatccacc cacacccgga cgccctcggc ttccatggcc
     6661 ggcgtgagcg cagcatccac ccggtcgatc agcatcccat ccaacctccc caagaccgat
     6721 tggtagaatc ggctcacccc caagggtgag acctcgactt tagcccactg gagaaacttg
     6781 tccgtgggtc cctttagcga ggcaccgccc accacggggc tcacggcaac gagcggcacc
     6841 ttcgtgttcc tcagacgctc gcggatcccg ggaacggcca aaatggga
//
LOCUS       DBNGZC010000880         2686 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2130509, whole genome shotgun sequence.
ACCESSION   DBNGZC010000880 DBNGZC010000000
VERSION     DBNGZC010000880.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2130509"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1352
                     /locus_tag="AC16G0_15275"
     CDS             <1..1352
                     /locus_tag="AC16G0_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family 51"
                     /protein_id="HIR5101016.1"
                     /translation="EQFFTGGGLHTFANYERDENRRVYSLREGLVQSVNLVYIRLMRD
                     IVRYHTARLPYDADRLFADPDDPTRHRLLEEIAEAEAKGVLAKAYQTYRTLSPREVEA
                     RLLGRQAKDERHQAMLFFAWQSGVGMEALGRWLEPRVGPVPPEQLLRLIKAYGNPELT
                     LADFGFLLGRHPLEVWCAGEVMHRPGISWSDLLARSQTPRRIASAWLFKTRNRHAQDL
                     RLRIRLEEDAFARMTPSWRRLGFPFEHLVPSFATAIGASSDRPDALAELMGTILNDGV
                     RLPTLDVRTLRFAAGTPYETVLERAPGTGLRVMPAPVARILRKVMTGVVTQGTARRVA
                     GAFANSQGTPIEVGGKTGSGDNRYKTFARGGELIASRPVSRTAAFAFYIGDRYVGVIT
                     ASVSGKEAGNYSFTSSLPVAILGLLAPAIETRLQPNLSDVERTPPATQHTVGMLLPG"
     gene            complement(1356..2429)
                     /locus_tag="AC16G0_15280"
     CDS             complement(1356..2429)
                     /locus_tag="AC16G0_15280"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR5101017.1"
                     /translation="MEDITARKQAEDALRKFAHVVEQSADTVIIADPNGIIEYVNPAF
                     EQLSGFAQEEAVGKTPGILKSGAHDAQFYDQLWRTIRSGGVFRSVFTNRKKDGDLFYE
                     EETITPVTDAQGGIVHFVSVGRDITGRVQAEGQLRRSREQLRSLAAHLQSVREEERSR
                     IAREIHDELGQVLTGLKFDLAWLARGLPDDSMALKEKTRGMLSLVDDTIRTVRRILTD
                     LRPSVLDELGLAAAIEWQAQEFQTRTGIECRVSTGLTELSLDDETSTAIFRICQESLT
                     NAARHAEATRISVSLRAEEDHLILTVEDNGKGITEEEQTRTTSLGLLGMQERAFLLGG
                     QLTIVGRPGGGTTVTVRMPLRAP"
     gene            complement(2475..>2686)
                     /locus_tag="AC16G0_15285"
     CDS             complement(2475..>2686)
                     /locus_tag="AC16G0_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR5101018.1"
                     /translation="ANPTLARMMGYASPQALMAAVTNIAQQLHVNPQRRAEFRRLLDE
                     HGLVQGFEMQVRRQDGSLMWVSASA"
BASE COUNT          435 a          892 c          855 g          504 t
ORIGIN      
        1 gggagcagtt cttcaccggt ggcggcctcc acacctttgc caactacgag cgggacgaga
       61 atcggcgggt ctattccctc cgagagggat tggtccagtc ggtgaacctg gtgtacatcc
      121 ggctgatgcg cgatattgtg cgataccata ccgcccgctt gccctacgat gcagacaggc
      181 tgttcgccga ccccgacgac cccactcgac atcgcctgct ggaggagatc gcggaagcgg
      241 aggccaaggg ggttctggcg aaagcgtatc agacctaccg gaccctttcg cctcgagagg
      301 tggaggcgcg gctcctgggt cgacaggcga aggacgagcg acaccaggcc atgctgttct
      361 tcgcctggca aagcggggtg gggatggagg ccctgggccg gtggctcgag ccgcgcgtgg
      421 gtccggtgcc gccagagcaa ctcttgcggc tgatcaaagc ttacgggaat ccagagctca
      481 ccctggcgga tttcggattc ctgctgggcc gccatccgct ggaggtctgg tgtgcgggcg
      541 aggtgatgca caggccgggg atctcctggt ccgacttgct cgcccgtagc cagacgccgc
      601 gccgcatcgc ctcggcgtgg ctcttcaaga cgcggaatcg tcacgcccaa gacctgcgcc
      661 tgcgcatccg cctcgaggag gacgcatttg cccgcatgac gccctcctgg cgccgcctcg
      721 gcttcccctt cgagcacctg gttccctcgt tcgccacggc catcggcgcc tcctcggacc
      781 ggcccgacgc actggccgag ttgatgggaa ccatcctgaa cgacggggtg cggctgccca
      841 cgcttgatgt gcggactctg cgctttgcgg ccggcacgcc ttatgaaact gtcctggagc
      901 gcgcgccggg gaccggcctg cgcgtcatgc cggcaccggt ggcccgcatc ctccggaagg
      961 tgatgacggg ggttgtcacc cagggaaccg ctcgccgcgt ggccggcgcc ttcgcgaact
     1021 cccaggggac acccattgag gtgggtggca agaccgggtc aggcgacaat cggtacaaga
     1081 cttttgcccg gggcggcgag ctcatcgcct cacgccccgt gagccgcacc gccgccttcg
     1141 ctttctacat cggcgatcga tacgtcgggg tcatcacggc ctccgtcagc ggaaaggagg
     1201 ccggcaacta cagcttcacc agctccctgc ccgtcgccat cctgggattg ctggccccgg
     1261 ccatcgagac ccgcctgcag cccaacctga gcgacgtgga gaggacgccg ccggccacac
     1321 aacacaccgt cggaatgctg ttgcctggat gatcgtcagg gtgccctgag cggcatccgt
     1381 accgtgacgg ttgtaccgcc ccccggtcgg cctacgatgg tgagttgtcc gcccagcagg
     1441 aaggcccgtt cctgcattcc caggagtccg agggaggtcg tccgcgtctg ctcctcttcg
     1501 gtgatgccct tgccgttgtc ttcaaccgtg aggatgaggt gatcttcctc ggcgcggagg
     1561 ctcacgctga tgcgcgtggc ctcggcgtgg cgggcagcgt tggtcaggct ttcctggcag
     1621 atgcggaaga tcgccgtgga ggtttcgtcg tccagggaca gctccgtcag ccctgtgctc
     1681 acacggcact cgatgcctgt gcgagtttga aactcctgcg cctgccattc tatggcggcc
     1741 gccaagccga gctcatccag gacgctgggg cgcaggtcgg ttaagatccg acggaccgtg
     1801 cggatcgtgt catccacgag cgagagcatc ccgcgggtct tctccttcag ggccatcgaa
     1861 tcatcgggca gcccgcgggc cagccatgcg aggtcgaact tcaagcccgt caggacctgc
     1921 cccagttcat cgtggatctc tcgggcgatc cggctccgct cctcttcccg cacggactgg
     1981 aggtgagcgg ccagagagcg caactgctcc ctggacctgc gtagttggcc ttccgcctga
     2041 actcgcccgg tgatgtcacg accaacagac acgaaatgga cgatcccgcc ctgagcgtct
     2101 gttaccggcg tgatcgtctc ctcttcatag aacaggtctc catccttctt cctgttcgtg
     2161 aacacgctgc gaaacacccc tcccgagcga atcgtccgcc agagctggtc atagaactgg
     2221 gcgtcgtgtg cacccgactt gagtatcccc ggcgtctttc ctacggcttc ctcttgggca
     2281 aaaccggaca actgctcaaa ggccgggttg acatactcga tgatgccgtt cggatcggca
     2341 atgatgacgg tgtccgctga ctgttcaaca acatgggcaa acttccggag cgcatcctcc
     2401 gcctgcttgc gggcggtgat gtcctccatg acgccctcgt agtggcgtac gctgccctgc
     2461 gcatcgtaca cagctcaggc actggcggac acccacatca ggctgccgtc ctgccgccgg
     2521 acctgcatct cgaatccttg caccagcccg tgctcatcca ggaggcgtct gaactctgcg
     2581 cggcgctggg gattcacgtg aagttgttgc gcgatgttgg tgaccgcggc catgagtgcc
     2641 tggggagagg cgtagcccat catccgcgcc agggtggggt tggcgg
//
LOCUS       DBNGZC010000881         2206 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2620013, whole genome shotgun sequence.
ACCESSION   DBNGZC010000881 DBNGZC010000000
VERSION     DBNGZC010000881.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2620013"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="AC16G0_15290"
     CDS             complement(<1..327)
                     /locus_tag="AC16G0_15290"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5101019.1"
                     /translation="MTRVFRIPWNGHVLAGDVLAGQEPPRLLVLHGGGQSNRERFRLL
                     RDHFFNHGVGSVAFDCVGHGETGGDLKQSSLRGRTAQACAVIDALSLPQPFSVLGASM
                     GGYTAVT"
     gene            complement(400..>2206)
                     /locus_tag="AC16G0_15295"
     CDS             complement(400..>2206)
                     /locus_tag="AC16G0_15295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5101020.1"
                     /translation="EPTEDVVTRAQREGCTVLLANHTTLVARDGREWPIEDSAAPITD
                     SVGEVSGVVVVFHDVTEKRRAPAALRESEDRLRLAVESAELGTWDFDPVTGSLTWSDR
                     CKALFGMPADARVDHAIFLERLHPEDRERIHALVQQALDPGGSGLFKAEYRVRWPEGT
                     ERWILAGGRAFFGSVDRQPRAVRFIGTALDITARKQAEEALHQAHNLLEQRVRERTAE
                     LSAANTALAHRSDQLRILAAELTLAEQRERKRLATVLHDEHQQLLVAAKIRVSLLERM
                     ADPRVRDAGHEVATLLQEAIENARSLTRELSPPVSLTGGLAPAVDWLVRWMEQKYHLA
                     VETQVDETAVPASDELTALLFQSMRELLFNIVKHTTVQTARVEIAREAGQLRLSVSDA
                     GGGFYPPDLRVEGGTGGGFGLFSIRQRVELLGGRLDIDSDYGRGSRFTLWVPLRPEPA
                     DHPAGAPVPKRSAGKQGKRRGKDDEPALKKVRVLLADDHTMVRQALARMLSQEPDIEV
                     VGEASDGKLAVELTRQLHPDVVAMDVNMPVMDGIQATQAIHAEFPDVRVIGLSMLEEG
                     EERSAMREAGAVGHLSKSGPSEALTAAIRACIRGG"
BASE COUNT          341 a          796 c          697 g          372 t
ORIGIN      
        1 ggtcaccgcg gtgtagcctc ccatgctggc gcccaagacg gagaatggct ggggcaggct
       61 cagggcgtca atgacggcgc aggcttgggc ggtccgcccc cggaggctac tttgtttcag
      121 gtcgcccccg gtctccccgt gcccgacgca gtcgaaggcc accgacccca cgccatggtt
      181 gaagaaatgg tcccggagca gccggaaccg ctcgcgattc gactggccgc ccccgtgcag
      241 caccaggagc cgcggcggct cctggccggc cagcacgtct ccggcgagca catgcccgtt
      301 ccagggaata cggaagacgc gcgtcatctt cccctaccga gagggatcaa gccctcccac
      361 accacacctg cgctccggcc ccccctggcg acttcgcgct cagcctccac gaatgcaggc
      421 acggatcgcc gccgtcaggg cttccgatgg cccgctcttg ctcaagtgcc ctaccgcccc
      481 ggcctcccgc atcgcgcttc tctcctcgcc ctcctcgagc atcgagaggc cgatcacgcg
      541 cacgtcgggg aattcggcat ggatggcctg ggtcgcctgg atgccatcca tcaccggcat
      601 gttcacatcc atcgccacca cgtccggatg aagctgccgc gtgagctcta cggccagctt
      661 gccgtccgac gcctcaccca caacttcgat gtccggttcc tggctcagca tgcgcgccag
      721 tgcttgccga accatggtgt gatcgtccgc aagcagaacc cgcaccttct tcaaggccgg
      781 ctcgtcgtcc ttgcctctcc tcttcccctg cttgcctgcc gagcgcttcg ggacgggcgc
      841 ccccgcggga tggtccgccg gctcggggcg aagcgggacc cagagcgtga accggctgcc
      901 ccggccatag tcactgtcga tgtccaggcg gccccccagg agttccactc gctggcggat
      961 gctgaagagg ccaaaaccac ccccggtgcc tccctccacg cggaggtccg gcgggtaaaa
     1021 gccgcccccc gcgtccgaca cgctgaggcg tagctgcccg gcttcccgtg cgatctctac
     1081 ccgggcggtc tgcaccgtcg tatgcttcac gatgttgaac agcagttccc gcatggattg
     1141 aaagagcagt gcggtcaatt cgtcgctggc tggcacggcc gtttcgtcca cctgcgtttc
     1201 caccgcgaga tggtacttct gctccatcca ccgcaccagc cagtccacgg cgggggccag
     1261 accaccggtg agcgaaaccg gcggagagag ttcccgggtg agggagcggg cgttctcgat
     1321 ggcctcttgg aggagggtgg caacctcgtg gcccgcgtcc cgcacccgcg ggtccgccat
     1381 ccgttccagc aggctcaccc ggatcttcgc cgccaccaag agctgctggt gttcatcatg
     1441 caggaccgtc gccagccgct tccgctcccg ctgctcggcc aaggtgagct ccgcagcgag
     1501 gatgcgaagc tggtcggatc ggtgggccag cgctgtattc gcggcggaca actcggccgt
     1561 ccgctctcgg acccgctgct ccagcaggtt gtgggcttgg tgcaaggcct cctcggcctg
     1621 tttgcgggcg gtgatgtcca aggcggtgcc gatgaagcgc acggcacggg gctgacgatc
     1681 cacgctccca aagaaggccc ggcccccggc aagaatccag cgctcggtcc cctcgggcca
     1741 ccggacgcgg tattcggctt tgaatagccc gctgcccccg gggtcgagcg cctgctggac
     1801 aagcgcatgg atgcgctcgc gatcctcagg gtgcagccgt tcgagaaata tggcgtgatc
     1861 cactcgggcg tcggcaggca tgccgaagag cgccttgcag cgatccgacc aggtgaggct
     1921 gcccgtgacc gggtcgaagt cccaggtgcc taactccgcc gattcgacgg cgaggcgcag
     1981 ccggtcctcg ctctcgcgca aggccgccgg cgcacggcgt ttctcggtga cgtcgtgaaa
     2041 cacgacgacc acgccggaaa cctcgccgac actatctgtg attggcgcgg cgctgtcttc
     2101 aatgggccat tcgcgaccgt cgcgagctac cagtgtggtg tggttggcca gcaggacggt
     2161 gcagccctca cgctgcgctc gcgtcacaac gtcctcggtg ggctcg
//
LOCUS       DBNGZC010000882         2306 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_858156, whole genome shotgun sequence.
ACCESSION   DBNGZC010000882 DBNGZC010000000
VERSION     DBNGZC010000882.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_858156"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(423..1139)
                     /locus_tag="AC16G0_15300"
     CDS             complement(423..1139)
                     /locus_tag="AC16G0_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5101021.1"
                     /translation="MADPILRVRNLVAFYGDAQALWDVSFQVDPGELVVLLGANGAGK
                     TTTIRTISGMLRSAGGTVEFKGTPIHGLSSCRIVERGIGHVPEGRALFPQMTVRENLL
                     LGAYLPGPSRRRRANLDRVFSFFPVLKERQRQLAGTLSGGQQQMCAIGRGIMSEPSLL
                     MLDEPSTGLAPLLVEEIFRIIADIRREGVTVLLVEQHIERALELADRAFILADGRTVG
                     AGTGSELLANEDVRRAYLSV"
     gene            complement(1121..1882)
                     /locus_tag="AC16G0_15305"
     CDS             complement(1121..1882)
                     /locus_tag="AC16G0_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5101022.1"
                     /translation="MSALLEVRSLIKSFGGLRALDGISFKVEAGKILSIIGPNGAGKT
                     TLFNVITGAMLPDSGDILFEGRSIKGLKPNQTAQLGIGRTFQIVRPFPGLTTLDNVAL
                     AALAKAGTRREAQRRAEEILEITRLSSFANIASRNLTLARRKRLEIARAMALEPRIIL
                     LDEVMAGLTPAEVDETVALIKDLTKMGISAVAGVEHVMQAVRKISDWIVALNFGQWLA
                     EGTPEEVMRNPEVIRAYLGTHYADSKESRRWPIRS"
     gene            complement(1883..>2306)
                     /locus_tag="AC16G0_15310"
     CDS             complement(1883..>2306)
                     /locus_tag="AC16G0_15310"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5101023.1"
                     /translation="GIREDELAAEAMGVNTTRNKLAAFILSAVFPAVAGGLYAWRLSY
                     IDPSSAFPGQYEMQSILMTIFGGAGTIWGPLLGGAALSIMAEVIWARFAELHLLLFGS
                     LIVLVLLFMPEGAIPLLRRYVAKWKGHGTESPSKKAVA"
BASE COUNT          398 a          765 c          749 g          394 t
ORIGIN      
        1 gccggggtca cgcccctcgg atccctcctg gtcgggacga tcgcggaatg gttcggcgtg
       61 tcggtcgcgt acgcggtcgg cggggggctg ggggtggcct gtgttctcgc cctcggcgtg
      121 ctctggcggc gagtttggtc gcaacccgcg gcggcctcct aacctgaccc cctcccttct
      181 gggcggaacg ataaggatgt ctcgccggat cgcgtgatac gagttttcgg gaatgacctg
      241 ggcgatggtg gaacgaccga gcagagatcg acgcggccgg actcagccac gaggccgatg
      301 caatcctgtt cgtgaggtcg ggtggatttg gctcgggagg acccagcggc gccgggattc
      361 gcggggagac gtcaggattc tggagcctgc tcggaatatc cgatgggttc tcaggcccgc
      421 cttcagacgc tgagataggc cctgcgcacg tcctcgttcg ccagcagttc cgagcccgtg
      481 ccggccccca cggtcctacc gtccgccagg atgaaggccc gatcggccag ttccagcgcc
      541 cgctcgatat gctgctccac caagaggacc gtgacccctt ccctccggat gtccgcgatg
      601 atccggaaga tctcctcgac caagagcggc gccaatccgg tggacggctc gtccagcatg
      661 agcagcgacg gttcggacat gatcccgcgc ccgatggcgc acatctgctg ctgcccgccc
      721 gagagcgttc ccgccagttg ccgctgccgc tccttgagca ccggaaagaa ggagaagacc
      781 cggtccagat ttgcccggcg ccgacgggag ggccccggca gataggcgcc gaggagcaga
      841 ttttcccgga cggtcatctg gggaaagagc gcgcgcccct ccggcacgtg tccaatgccc
      901 cgctccacga tccggcagga agagagaccg tggatcggcg tccccttgaa ctccaccgtg
      961 cctcccgcgg accggagcat gccggaaatc gtgcggatgg tggtggtctt gccggctccg
     1021 ttggcgccca agagaaccac aagctcaccg gggtccacct ggaaactgac atcccagaga
     1081 gcctgggcgt ccccgtagaa ggccaccaga ttgcgcaccc tcaggatcgg atcggccacc
     1141 gtcgtgactc ctttgagtcg gcgtagtgag tgccgaggta tgcgcgaatg acctccgggt
     1201 ttcgcatgac ctcctcaggg gtgccctcgg ccagccattg gccgaagttg agcgcgacga
     1261 tccagtccga gatctttcga acggcctgca tcacgtgctc caccccggca accgccgaaa
     1321 tgcccatctt ggtgaggtcc ttgatcaggg ccactgtctc gtccacctcg gccggcgtga
     1381 gccccgccat gacctcgtcc agcaggatga tccggggctc cagggccatg gcgcgggcga
     1441 tctccagccg cttgcgtcgg gccagggtca agttgcggga tgcaatgttc gcgaaggaag
     1501 acaatcgggt gatctccagg atctcctccg cccgccgctg ggcttcgcgc cgcgtgcccg
     1561 ccttggccag ggcagccagc gccacgttgt ccagcgtggt gagacccggg aaggggcgga
     1621 cgatctggaa ggtccgaccg atgcccagtt gggcggtctg attcggcttc aaccccttga
     1681 tcgaccgtcc ctcgaacagg atgtcccccg aatcggggag catcgcgccc gtgatcacgt
     1741 tgaacagcgt cgtcttcccc gccccgttgg gtccgatgat gctgagaatt ttccccgcct
     1801 ccaccttgaa ggagatgccg tccagcgcgc gcagacctcc gaaggacttg atcagcgacc
     1861 gcacctcgag cagggcggac attcaggcca ccgctttctt ggagggggat tcagtgccat
     1921 gtcctttcca ctttgcgacg tatcgccgga gcaacgggat cgccccttcc ggcatgaaca
     1981 agagcaccag aacgatcaag gacccgaaca gcagcaggtg cagctcggca aatcgagccc
     2041 agatcacctc ggccatgatg gagagggcgg ccccgcccag gagcggtccc cagatcgttc
     2101 cggcaccgcc gaagatggtc atcaggatcg actgcatctc gtactggccc ggaaaggccg
     2161 acgacgggtc gatgtagctg aggcgccagg cgtagagccc gcctgccacc gccgggaata
     2221 cggccgagag aatgaaagcg gccagcttgt tgcgggtggt gttgacgccc atggcttcgg
     2281 cggccagctc gtcttcccgg atcccc
//
LOCUS       DBNGZC010000883         7864 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_372396, whole genome shotgun sequence.
ACCESSION   DBNGZC010000883 DBNGZC010000000
VERSION     DBNGZC010000883.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7864)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7864)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7864
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_372396"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="AC16G0_15315"
     CDS             complement(<1..1153)
                     /locus_tag="AC16G0_15315"
                     /inference="COORDINATES: protein motif:HMM:NF012678.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR5101024.1"
                     /translation="MTTSSSVMPFVFSIPTNIVFGVGALDSIGTHAKSLGFKRPLIVT
                     DKIMSRGAGITQLLAKLTEAGLEAAIWDDITAEPIDTTLTEGVTRYRSERCDGLIGFG
                     GGSAMDTAKGVGVLAITGDDDPTPYQRLGTKTVPGCVPVVAIPTTAGTGSEVTSVCVF
                     TSQRTGHKLPIKHPSLLPRIAIVDPRLMLSMPPKVTMATGIDALSHATECFTQVREHP
                     FSDTLSLQAIRLIVENLPKAIANGQDETARSNMALAATMAGTAFEIGGLQFHAYAQAL
                     GAKYHAPHGVTCGIALRAGLRQILPTATAKLARMAWAFAIDTRGMSQTQAAAKAVDAA
                     ILFINEMGIPKVTEAMRATPSDIPMLLKETLETTGVPIPGETATAVWQEI"
     gene            complement(1324..2460)
                     /locus_tag="AC16G0_15320"
     CDS             complement(1324..2460)
                     /locus_tag="AC16G0_15320"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR5101025.1"
                     /translation="MANMGHRERVMRALNHQEPDRVPVDLGATRSSSLVVEAYENLNR
                     HLGVEAPTRIFSKWLHIAHPSEVMLRRFDIDTRGVGQGGPDNWQDKTNSGDSYEDEWG
                     VVRSRPAGSLYYDLTKSPLAGDVDVRDVETFPWPDPHDPGRCRGLAEAAARLHTETDY
                     AVVLNMPGGIVHQAQFLRGFEDWFADMIANPVFFQALMEKLADLWIEMARDELDAVGG
                     NVDICFCGDDVAFQDGAMMSMDLYRKMIKPHHKRLFGYIKSRSPAKIVYHTCGSVVHL
                     IPDLIEMGVDALNPVQVSAKGMDTKRLKQEFGKDICFWGAIDTQRVLPFGKPGEVVAE
                     VKRRIEELGPGGGYVLCAVHNIQPDVSPENICAMYDAAREYGRY"
     gene            complement(2777..3808)
                     /locus_tag="AC16G0_15325"
     CDS             complement(2777..3808)
                     /locus_tag="AC16G0_15325"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008346255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR5101026.1"
                     /translation="MSTTHLPVTAETSPRGAKVREITYAEAIAEALRTEMRLDPRVFV
                     MGEDVGNFGGLFGGTKDLLREFGPERVRDTPISEAALIGAGVGAAITGMRPMVEIQFM
                     DFTACAMDQIVNQAAKIRFMLGGKAEVPLVIRTPYGTGLKLASQHSQSLEAWFVHVPG
                     LLVAMPSTPADAKGLLVTALRDPNPVIFLEHKLLYPTRGPVREEQYTIPLGVAEVKRS
                     GKDVTVVATGLMVHKALTAAEALAAEGLDVEVVDPRTLVPLDKATIIESIKKTGRLVI
                     VHEAVKRGGFGGEIAGVIAEEAFDYLDAPIKRVAGRNVPIAFNEVLEQKAVPSVEDIA
                     AAVRSLVAS"
     gene            complement(3805..4776)
                     /locus_tag="AC16G0_15330"
     CDS             complement(3805..4776)
                     /locus_tag="AC16G0_15330"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014354585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR5101027.1"
                     /translation="MPDRIARLDMLKMMVRIRAFEMKLAELVKNLTIKGTMHPYVGEE
                     AIAAGVCAALRPDDYITSTHRGHGHALAKGADMKRMMAELLGRHTGYCKGKGGSMHIA
                     DLSLGMLGCNGIVGGGMGITAGAALSIKLRGTDQVSVGFFGDGGANTGITHESMNLAA
                     TWRLPAIFVCENNQYALSMPYAKAVAMPDIAARGAGYGMPGEVVDGNDVLAVWSATRQ
                     AAERARQGQGPTLLECKTYRWLQHSLRVKGPDRPAEEEAAWKARCPIDHLRRHLAQAG
                     VLDDAGFAAIQAAVAKEVEESVVFALASPEPPLEEMELDVYAGRFIE"
     gene            5400..5660
                     /locus_tag="AC16G0_15335"
     CDS             5400..5660
                     /locus_tag="AC16G0_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101028.1"
                     /translation="MTVTGLLRARPEDCYFWATHMGAELDLLVRRGTVTLGFEFKRAD
                     TPALTRSMRVALEDLRLKYLYVMHPGADTFPLAPQVTAVALP"
     gene            6322..7317
                     /locus_tag="AC16G0_15340"
     CDS             6322..7317
                     /locus_tag="AC16G0_15340"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR5101029.1"
                     /translation="MESMTKRERVQAALSGAPVDRIPISFWRHFPDLDLDPMALAEAL
                     VAFHRRYDLDFIKVMPNGVYGVEDWGCEIAYQGGTSGARTCVRHAVQRIGDWERLHPL
                     KPTAGALGRELACLRAVCAGRGDDAPVLQTIFSPFTLARKVAGPDLVGETMLRDPDRL
                     HAALGVISLTVETYVGACLDAGADGIFFASQTATPEVMSAEEHERFVEPYDLRVLAAA
                     RRRGAIVLLHLHGDRPYLISLAARYPVQALNWHDRRTSPSLAEAAAQVSQCLVGGLDE
                     RGAMITATPQEVRAQVQDAVAQCRGRRLILGSGCVVDLQVPEANLKAAREAVEGL"
     gene            7338..>7864
                     /locus_tag="AC16G0_15345"
     CDS             7338..>7864
                     /locus_tag="AC16G0_15345"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5101030.1"
                     /translation="MQRRILVVLVTVTVILGLLSPAHAAEPLGELHVAIPWTPENLDP
                     SMNLSSIRAQVGMSLFDSLVGRDAQRHIVPELAESWKLLDDNTWEFRLRKGVVFHDGE
                     PFNAEAVRFTIQRVLDPNQKSPNRANIAEIAKIDVIDDYTVRLVTRQPYAPLLNRLLD
                     FPILPPKYTAEKGDQA"
BASE COUNT         1310 a         2624 c         2493 g         1437 t
ORIGIN      
        1 agatttcctg ccagactgcg gtggccgtct cccctgggat gggcacgccg gtggtctcga
       61 gcgtctcctt gagcagcatg ggaatgtcgc tcggggtggc ccgcatcgcc tccgtcacct
      121 tggggatgcc catttcattg ataaatagaa tcgccgcgtc cacagccttc gcggccgctt
      181 gcgtctggct catgccgcgg gtgtcgatcg cgaacgccca ggccatgcgg gcgagcttcg
      241 ccgtggcggt tggcagaatc tggcgtagcc ctgcgcgcag cgcgatgccg caggtcactc
      301 cgtgcggcgc atggtacttg gcgccgagcg cttgcgcgta cgcatggaac tgtaggcctc
      361 caatttcgaa tgccgtcccc gccatcgtcg cggcaagggc catgttgctc cgtgccgtct
      421 cgtcctgtcc gttggctatg gccttcggca ggttctccac gatcaaccgg attgcctgga
      481 gagacagcgt gtccgagaac gggtgttccc ggacctgcgt gaagcactcg gtggcatgtg
      541 acaaggcgtc gatcccggtt gccatggtga ccttcggcgg catggacagc atcagtcgcg
      601 ggtccacgat ggcgatcctg gggagcaggc tgggatgctt gatgggcagc ttgtgcccgg
      661 tgcgctggct cgtgaacacg cagaccgacg tgacctcaga tcctgtcccg gcggttgtgg
      721 ggatggcgac gaccggcaca cagccgggca ctgtcttggt accgagccgc tgatagggag
      781 tcgggtcgtc atccccggtg atggccagaa cgcccacgcc cttggcagtg tccatggcgc
      841 tcccgccgcc gaatccgatg aggccatcgc agcgctcgct gcggtaccgt gtcacgcctt
      901 cagtcaaggt tgtatcgata ggctctgcgg tgatgtcgtc ccatatggca gcctccagcc
      961 cggcctcggt caacttggcc aagagctgcg taatgccagc cccccgagac atgatcttgt
     1021 cggtcacaat gagggggcgt ttgaaaccca gggacttggc gtgtgtgccg atcgaatcga
     1081 gtgctccaac tccaaagacg atattggttg ggatgctgaa cacgaatggc ataacactgc
     1141 tggatgttgt cattggcgtg agtcctcctc gatgtatgta gctgtggtga tctgtgggcc
     1201 gcatcgcctc ccagtccgag aatggatagc gtgagattca gatctcgcct ccgagtgagg
     1261 tctggagcga tgaatagagc tcgtgccccc cgccggcccg accgtcgtca tcaagactcg
     1321 cgctcagtac cggccgtatt cccgggccgc gtcgtacatg gcgcagatat tctcgggcga
     1381 gacgtccggc tgaatgttgt gcaccgcgca caggacgtag ccgccccccg gccccaactc
     1441 ctcgatccgc cgcttcactt cggcgacgac ctcgccgggc ttgccgaagg gcaggacgcg
     1501 ttgagtgtcg atggcccccc agaagcagat atccttgccg aactcctgct tcagccgctt
     1561 cgtgtccatg cccttggccg acacctggac aggattgagc gcgtccacgc ccatctcgat
     1621 gaggtcgggg atgaggtgca ccaccgagcc gcaggtgtgg tagacgatct tggcggggct
     1681 gcgggacttg atgtatccga agagccgctt gtgatgaggc ttgatcatct tccggtagag
     1741 atccatggac atcatggcgc cgtcctggaa cgccacatcg tctccgcaga agcaaatgtc
     1801 cacgtttccc cccaccgcgt ccagttcgtc cctggccatc tcgatccaga gatcggcgag
     1861 cttttccatg agggcctgga aaaagacggg gttggcgatc atgtccgcga accaatcctc
     1921 aaatccccgc aggaactgcg cctggtgcac gatcccgccg ggcatgttga ggacgacggc
     1981 gtaatccgtt tccgtgtgca ggcgggccgc cgcctcggcc agaccccggc agcgtcccgg
     2041 atcgtgggga tccggccaag ggaacgtctc cacatccctc acgtccacat cccccgcgag
     2101 gggagatttc gtgaggtcgt agtacaggct ccccgcgggg cggcttcgca ccacacccca
     2161 ctcgtcctcg taggaatccc cggagtttgt cttatcctgc cagttgtccg ggccgccctg
     2221 accgacgccc cgggtgtcga tgtcgaaccg cctgagcatc acctcgctgg ggtgggcgat
     2281 gtgcagccac ttgctgaaga ttcgcgtggg ggcctccacg cccaggtgcc ggttcaggtt
     2341 ttcgtaggcc tcgaccacta acgaggagga gcgggtggcc cccaggtcaa caggcacccg
     2401 atccggctcc tgatggttca gcgcccgcat cacccgctcg cgatgaccca tgttcgccat
     2461 gatccctcct gccccaggcc ttccgccggt gaacctccgc acttcggcag cgcacgagcc
     2521 gccggaggcc tgcgccatgc acccgaatga gcacaattcg atccactgca ggggaagtat
     2581 aggccgagtg gtgcctgtgg atcaactggt cggcgaggat tgatcggaag ggatgtgaaa
     2641 tggcgcatga ctccgagtat gggccagttc gtggatatcc ttggtgtggg ttgccgattc
     2701 ttttggcgca cgaccccagg ttgcgccggc aagattcggg ggagggagac cggaagcggg
     2761 tccctaccgg aaagcgtcag gatgcgacca gggaccgcac cgcggcggcg atgtcctcga
     2821 cgctggggac ggctttctgt tccaggacct cgttgaaggc gatggggaca ttcctgcctg
     2881 cgacccgctt gatgggggcg tccaggtagt cgaaggcctc ctcggcgatc accccggcga
     2941 tctccccgcc gaagccgccg cgcttgaccg cctcgtggac gatcaccagg cgtccggtct
     3001 tcttgataga ctcgatgatg gtggccttgt ccagcggcac cagcgtccgc gggtccacaa
     3061 cctccacgtc aagtccttcc gccgccagcg cctcggcggc cgtgagggcc ttgtggacca
     3121 tcagaccggt ggccacgacc gtcacgtctt tccccgatcg cttgacctcg gccaccccca
     3181 gcggaatggt gtactgctcc tcccgcacgg gacccctggt cgggtagagc agcttgtgct
     3241 ccaggaagat gacggggttc gggtcgcgca aggccgtcac gagcaacccc ttggcgtccg
     3301 ccggggtgga gggcatggcc accagaaggc cgggcacgtg cacgaaccag gcctccaggc
     3361 tctgcgagtg ctgcgatgcc agcttcaggc cggttccata gggcgtccgg atgaccaggg
     3421 gaacctcggc cttgcccccg agcatgaagc ggatcttggc ggcctggttc acgatctggt
     3481 ccatggcgca ggcggtgaag tccatgaact ggatctccac catcggccgc ataccggtga
     3541 tggccgcccc gacacccgcc ccgatcaggg ccgcctcgga gatgggcgtg tcccgcaccc
     3601 gctccgggcc gaattcccga agcagatcct tggtcccgcc gaagaggccg ccgaagttcc
     3661 cgacgtcttc ccccatcacg aagacgcgcg ggtcgagccg catctcggtc cgcagcgcct
     3721 cggcgatggc ctcggcatag gtgatctcgc gaactttcgc cccgcgcggg gaggtctcgg
     3781 cagtcaccgg caggtgtgtt gtactcattc gatgaacctc cccgcgtaca catccagctc
     3841 catctcttcc agcggcggct ccggactggc cagcgcgaag accaccgatt cctcgacctc
     3901 cttggcgacg gcggcctgga tggcggcaaa gccggcatcg tccaggacgc ctgcttgcgc
     3961 gagatgcctc cgcaggtggt cgatcggaca cctggccttc caggcggcct cctcctcggc
     4021 agggcggtcc ggccctttca cccgcaggct gtgctgaagc caacggtacg tcttgcactc
     4081 gaggagtgtc gggccctggc cttgtcgggc gcgctccgct gcctgccggg tggccgacca
     4141 gaccgccagg acgtcattac catccaccac ctcgcccggc atgccgtagc ccgcgcctcg
     4201 cgcagcgatg tccggcatgg ccaccgcctt ggcgtagggc atggacagcg cgtattggtt
     4261 gttctcgcag acgaagatgg ccggcaatcg ccaggttgcg gcgaggttca tggactcgtg
     4321 ggtgatcccg gtgttggccc cgccgtcccc gaagaagccg acgctcacct ggtccgtgcc
     4381 gcgcaacttt atggagaggg ccgcccccgc cgtgatcccc atgccgcccc ccacgatgcc
     4441 gttgcagccc agcatcccca agctgaggtc ggcgatgtgc atcgagccgc ccttcccctt
     4501 gcagtacccg gtgtgccggc ccaggagctc ggccatcatg cgcttcatgt cggcgccctt
     4561 cgccagggcg tgcccgtgtc cccggtgagt gctggtgatg taatcgtcgg gccgcagggc
     4621 ggcgcacacc ccggccgcga tcgcctcctc gccgacatag ggatgcatcg tccccttgat
     4681 ggtcagattc ttgaccagct ccgccagctt catctcgaag gcccggatcc ggaccatcat
     4741 cttcaacatg tccagcctgg cgatccggtc gggcatctca ccctggcccg ttccttcacc
     4801 ggacttggcc cgagccggag tatcgcttgc cggcttggtg accctctgcc tcttcttgac
     4861 tgtcgccatt gcctgctcca ctctcgagag gaccgaacct cctcaggatg ccttgacccc
     4921 gcccaccgct cgccccgggg catccccggg gagcgaggct ccttcaggcc ggctaccttc
     4981 tggcctctac cgcttcacta tgcgccaata acgccggccc ggcaagcggc ggtatgccca
     5041 gtgggcgtag gctgagttga aatatctcgt agagcagctc ggcaggggcg atcttgcgcc
     5101 ccctcgtcag gcattccggg aacgcgataa aacgggctga actgaaccgc tggcctacaa
     5161 gctctgggga ttgtatctga accctgaagc cgggggaaga gatttgcggg tggaagtgaa
     5221 aggagacgcc tgggaggagc ggggcgggca gcacggacgc ggtggtgctc cgacctgctg
     5281 gagacacgga gcagacagtg cggcgaaggg gagccgttgt tgtccgggcg gacggctggg
     5341 gcgtggatgg gtcagatttc cttcatccat gaaatctgtg cagcaatcgc tcagatttca
     5401 tgaccgtgac ggggctcctg cgcgcacggc cggaggactg ctacttctgg gcgacccaca
     5461 tgggggcgga actcgacctg ctggtccgcc gtggcaccgt gacgctcggg ttcgaattca
     5521 agcgggccga caccccggcg ctcacaagga gcatgcgcgt cgccctggag gatctccgcc
     5581 tcaaatacct gtacgtcatg catccgggag ccgacacatt ccctctggcg ccacaggtca
     5641 ccgcggttgc cctcccctag gcaccgagcg tccttcgccc cctgcgctga tagcgcgccc
     5701 cctccaatga acgaaacccg actgctcaag ggacaccgcc gcggggcgcg gcgggaagta
     5761 gacacccccg catttgtagg ctgagggcat gttgttgacc agatgggcat tcttcctgtc
     5821 gcctaagatc ctcctgcgat cagtgcggcc tgtcgatccc aggcgtccca tccagatggg
     5881 cgggtgtgcc ccgtccgttt gcgagcggcc gaggactccc cagcggcgca ggtcgtcctg
     5941 aacctccgac gaaaagcccg tccccggctc cagacatgcc ggctccactc agactccgtc
     6001 cgcacacgcc aggttcccct tcacctctct gaaagtcacc cccagggtcc ccgcctcgag
     6061 gtgttgcgga gcgcagccta tcgtactata aactcgcaaa gctcaatcct gtcctcaagg
     6121 cggtcgacgc ctccaaggac acgcagaggc agaagaaatt ccttccccgg acggcgcgca
     6181 tctttcatca ggatccgccg gctgccgccc attacgccga gaacagcatc cggggcccgt
     6241 gacgatgcct gatccggagg gcggcgacag accggcggtc cccgccactg gttgttgcgg
     6301 gtctatgctg atggaggcct catggaatcc atgaccaagc gggaacgtgt gcaggcggcc
     6361 ctctcgggcg cgcccgtgga tcgcatccct atcagtttct ggcgccactt cccagatctc
     6421 gacctggacc cgatggccct ggccgaggcc ctcgtggcct tccaccggcg atatgacctg
     6481 gacttcatca aggtcatgcc gaacggggtg tacggcgtgg aagactgggg gtgcgagatc
     6541 gcctaccagg ggggaacctc gggcgcccgg acgtgcgtcc ggcacgccgt gcaacggatc
     6601 ggggactggg agcgtctcca tcccctcaag ccgaccgcgg gcgcgctggg gcgggaactc
     6661 gcctgtctca gagcggtttg tgcggggcgg ggagatgacg cgcccgtgct ccagacgatc
     6721 ttttctccat tcaccttggc ccgaaaggtg gcaggcccgg acctggtggg agagacgatg
     6781 ctacgcgacc cggaccgcct gcacgcggcc ctgggagtga tcagcctcac ggtcgagaca
     6841 tacgtcggag cgtgcctgga cgccggggct gacggcatct tcttcgccag ccagactgca
     6901 acgcccgagg tgatgagtgc ggaagagcac gaacgattcg tggagccgta cgatctgcgg
     6961 gtcctcgccg ccgcgcgacg caggggggcg atcgtcctcc tccacctgca tggggatcga
     7021 ccctacctga tcagcctggc cgcaaggtat cccgtccagg cgctgaactg gcacgaccgg
     7081 cgcacctcgc cgtcccttgc cgaggctgcg gcccaggtgt cgcagtgcct ggtgggagga
     7141 ctcgatgagc gcggcgctat gatcacggcc acgccgcagg aggtccgtgc ccaggtgcag
     7201 gacgccgtgg cccaatgccg tgggcgtcgt ctcatcctgg gatccggatg cgtcgtcgac
     7261 ctccaagttc ccgaggcgaa cctaaaagcc gctcgggaag cggttgaagg cctttgaccc
     7321 acaggagggt gttgatcatg cagcgtcgaa tcctcgtcgt ccttgtcact gtcacagtga
     7381 tcctgggcct actctctccg gcacacgcgg cagagcccct gggggagttg catgtggcca
     7441 ttccctggac cccggagaac ctcgacccca gcatgaacct ctcctccatc cgggcacagg
     7501 tcgggatgag tctgttcgac tcgttggtgg gcagggatgc acagagacat atcgtccccg
     7561 agttggctga gtcgtggaaa ctcctggacg acaacacgtg ggagttccgg ctccgcaagg
     7621 gcgtggtctt tcatgatggc gagcccttca acgccgaggc cgtccgcttc accatccagc
     7681 gcgtgctcga cccgaaccag aagtcgccca atcgcgccaa catcgccgag atcgccaaga
     7741 tcgacgtgat tgacgactac accgtcagac tagtcacgcg acagccctac gcgccgctcc
     7801 tgaatcggct tttggatttc ccgatcctgc cccccaagta caccgccgag aagggggacc
     7861 aggc
//
LOCUS       DBNGZC010000884         2212 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2030272, whole genome shotgun sequence.
ACCESSION   DBNGZC010000884 DBNGZC010000000
VERSION     DBNGZC010000884.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2030272"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..952
                     /locus_tag="AC16G0_15350"
     CDS             122..952
                     /locus_tag="AC16G0_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101031.1"
                     /translation="MTLLLGPMGCAPNPLAHSPSPSQLASATTGTIGVVPGRFPPEIK
                     LVTVTQSRALGAAKGAGLGFVTGAGTALGATAGMPYGCSGWVCGAALLVEAALVFVGG
                     TVGAVMGGVTGAMEPIPLKSIGPVEGDARTALADLGLQDAMATRVLRELQQRPPKQFL
                     LLPDDGPRTIDEDTDYRALESQGVGAVLEVAVRELGFESGEGEDPPLRLFLTLRARLI
                     RTTDGSTLYASTLDHRSESLKRAAWEANGFQPLRAAIDEAGRSFAERIAKTLFPIPED
                     "
     gene            1666..1926
                     /locus_tag="AC16G0_15355"
     CDS             1666..1926
                     /locus_tag="AC16G0_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HIR5101032.1"
                     /translation="MRPTRSSSTPKGRRRLRLTAVVERDRSGYVASCPQLQGCYTQGR
                     TYEEVVRNIRDAIRLHLEDRLALGEPLPTPGEVTLAVLDVAV"
     gene            1923..2072
                     /locus_tag="AC16G0_15360"
     CDS             1923..2072
                     /locus_tag="AC16G0_15360"
                     /inference="COORDINATES: protein motif:HMM:NF019540.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HIR5101033.1"
                     /translation="MSPHLPSVTAGQILRVLRRLDFEEVRSSGSHRILRNAEGKRVTL
                     PYHAG"
BASE COUNT          400 a          659 c          772 g          381 t
ORIGIN      
        1 acgccagtca gcgagatcgt cggggtgact gccatcggcg gagaggggtg ggggagtcag
       61 gccaacgcgg gtggcgaaga tgaagacgac tgcgcggcgg cggttcctgg tctggtgcgc
      121 catgaccctg ctccttgggc ccatgggctg cgccccgaat ccgctcgccc attctccatc
      181 tccatcgcaa ctggccagcg ccacgacggg aaccatcggg gtcgtcccgg gtcggttccc
      241 cccggaaatc aagctggtga cggtgacgca gagccgcgcc ctcggagctg ccaagggcgc
      301 aggcctgggt ttcgtgaccg gagcgggcac agcgctgggc gctaccgccg ggatgcccta
      361 cggttgcagt ggctgggtgt gcggggcagc gctgttggtc gaggccgcct tggtctttgt
      421 aggtggcacg gtgggcgccg tgatgggcgg ggtgacgggg gcgatggagc cgatccccct
      481 caagagtatt ggaccggtcg agggggacgc ccgaacggcg ctggcggacc tgggcctcca
      541 ggacgccatg gcgacccgcg tcctccggga actccagcaa cgcccaccga agcagtttct
      601 tctcctacct gacgacggcc cgcggacgat cgacgaggac actgactacc gcgcattgga
      661 gtcccagggc gtgggagccg ttctggaggt cgccgtccgt gaactggggt tcgagtcggg
      721 ggagggcgaa gatcctccct tgcgcctctt cctgactcta cgggcgcggc tcattcggac
      781 aacggacggg agcactctct acgcctccac cctcgatcac cggagtgaga gtctgaagcg
      841 cgccgcctgg gaagcaaacg gcttccaacc ccttcgggcg gcaatcgacg aagcgggccg
      901 gagctttgca gagcgcatcg ccaagactct cttccccatc cccgaggatt gacccttgag
      961 aagaacgccg taatggggga ggcagcaatt ttcgtcgtct tgagtcagac gggatgttgc
     1021 agtcttagtc gcgttgaggg caaggcggca ttcggaggcg ggagattggt ggaggagttg
     1081 gggagcatgg ggccgggggg aaacggggaa acggagaaac ggggaatcgg ggaaacggag
     1141 aagcggagaa tcggggaacc ggggacgagg gtgtgatgag gcgagcacta tgcggcttgg
     1201 ccttggctgc ctgattcttg cggttgctct gccggggctc ggactctcgc gggccgaggt
     1261 ggcgtaccgg ctgacctgtc agaatggaaa cggtgggacg attgccactc cgatggcgaa
     1321 caggtccgcc gaagaggagg ccgtccatct tcctccgccg accccacccg ctcccacctc
     1381 actggcgcca tcggccgttc ctgcgaatgc aactcagggt gttcacgaga ccgtccgatt
     1441 gcccgtcgag tttgccagcc ggacggaggc tgacaacctc tccgacagtc cgcgtgggtt
     1501 catcgtgaga ggcggctcgt ctgacgaaac agagacacgg ggcaaataat cactcggtgg
     1561 ctgcgccggg tgggagtatc ggggatggag gatggacatc tgcggccgaa ggacgtctgt
     1621 cggccccggc tgttcgtcac ggagggacga atgcccgtca gggccgtgcg acccacgaga
     1681 agctcgtcta ccccaaaggg gcgacgccgc ctccggttga ccgccgtggt ggagcgggat
     1741 cgatccggat acgtcgccag ttgcccgcaa ctccagggct gctatacaca gggacggacc
     1801 tacgaagaag tggtgcggaa tatccgcgac gccattcgcc tgcacttgga ggatcgcttg
     1861 gcgctcgggg aaccgcttcc cactccgggt gaggtcacct tggcggtcct ggacgttgca
     1921 gtatgagccc acacctcccc agcgttacgg ctgggcagat cctgcgggtc cttcgacgcc
     1981 tcgatttcga ggaggttcgt tcgagcggga gccatcggat actgagaaac gcggagggga
     2041 agcgcgtcac gctgccttac cacgccgggt aagtcctgca tcccaagatt ctgcagagca
     2101 tcctccgaga cgctgatatc tcagtggacg aattccgcga gcttctctaa gcacaaactc
     2161 ttcaccgcaa tgctgtaatg gtactaatgg gggacgcatt cacttcgacc ac
//
LOCUS       DBNGZC010000885         1764 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_372558, whole genome shotgun sequence.
ACCESSION   DBNGZC010000885 DBNGZC010000000
VERSION     DBNGZC010000885.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_372558"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="AC16G0_15365"
     CDS             <1..401
                     /locus_tag="AC16G0_15365"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR5101034.1"
                     /translation="HLGIPAARLGAAVNPGATARLVSLVGPAVAKEMLFGGQGLDAAH
                     ALRVGLVNHVVPDAELESFTLSLVHQIGVNAPLTIRHAKQAVNWIAVEGHRRPPRTIQ
                     EHFVRGFSSRDFKEGVAAFLARRPPKFIGR"
     gene            544..933
                     /locus_tag="AC16G0_15370"
     CDS             544..933
                     /locus_tag="AC16G0_15370"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR5101035.1"
                     /translation="MSAIEIGLRGRATTRVTPENTAQAWGSGDVPVFGTPSLVALLET
                     AAVNALAGHLQPGETTVGTWIEVSHLAASPVGVVVSAEAELTGVDGRTLTFALVAHDH
                     RQQVGEGRHRRVIVSRQRFLEKTAAKH"
     gene            complement(939..1250)
                     /locus_tag="AC16G0_15375"
     CDS             complement(939..1250)
                     /locus_tag="AC16G0_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HIR5101036.1"
                     /translation="MYAVCVTVHVLAEQVQGFIQATLENARQSRLTEPGNVRFDILQA
                     ESDPARFFLYEVYQEKEDFARHQQTPHYLKWKETVAPWMAVPRQGVRHLSLFPDEDGW
                     R"
     gene            complement(1359..>1764)
                     /locus_tag="AC16G0_15380"
     CDS             complement(1359..>1764)
                     /locus_tag="AC16G0_15380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101037.1"
                     /translation="QLTQAGIRTQLKVHEWTTYMNNLAYRHNGGPMWLIGWGNTTWDA
                     DYTYTPMFRTGQILANYWNPDFNSLLEEGQTNMNPKRRQEIYSKADKLFVEDAPCISL
                     YQQIDNYGVSRRLQWTARSDERIEGFSISIKQ"
BASE COUNT          297 a          587 c          561 g          319 t
ORIGIN      
        1 cccatctcgg catcccggcg gccaggctcg gggcggccgt caatcccggc gcgaccgcca
       61 gactcgtgag cctggtgggc ccggcggtgg ccaaggagat gctgttcggc ggccagggcc
      121 tggatgcggc gcatgccctg cgcgtgggcc tggtgaatca cgtcgtgccg gatgccgaat
      181 tggagtcgtt cactttgtcc ctggtccacc agatcggcgt caatgccccg ctcaccatcc
      241 gccacgccaa gcaggccgtg aactggatcg ccgtcgaggg gcaccgacgc cccccgcgga
      301 cgatccagga acacttcgtc cggggcttta gcagtcggga cttcaaggaa ggcgtggccg
      361 cgttcctggc gaggcgaccg cccaagttca tcggccgctg acaggctgct gaagaaggcc
      421 catctgcggc gttgtcccgc ctcggcactc gttgcggcgt acctccagta cgcctcgctc
      481 ggcctgcggc gtgccgcctt gcatctggac ctttttgagc agcctgatcg aggtgatcgg
      541 cagatgagcg cgatcgagat tggtctacgc ggcagggcca caacgcgggt cacccccgag
      601 aacactgccc aggcctgggg cagcggtgat gtcccggtct tcggcacccc cagcctggta
      661 gcgcttctgg aaaccgccgc ggtgaacgcc ttggccggac acctccagcc gggcgagacc
      721 acggtgggca cctggatcga ggtctcccat ctcgccgcgt cccccgtggg cgtggtcgta
      781 tccgccgagg cagaactcac cggcgtggac ggccggaccc tcacgttcgc gctggtcgcc
      841 cacgatcacc ggcagcaggt cggcgagggc cggcacaggc gggtgatcgt ctcacgccag
      901 cggttcctgg agaagaccgc cgccaagcac tgaccgcatt atctccaccc atcctcgtca
      961 gggaacaggc tcaggtggcg gacgccctgc cgggggaccg ccatccaggg ggcgacagtc
     1021 tctttccact tgaggtagtg cggcgtctgc tgatgacggg cgaagtcctc cttctcctgg
     1081 taaacctcat acaggaagaa ccgcgccgga tccgactccg cctgcaggat atcgaaccgg
     1141 acattgccgg gctccgtcaa ccggctctgc cgcgcgttct ccagcgtagc ctggatgaac
     1201 ccctggacct gctcggcgag gacatgcacg gtgacgcaga cagcgtacat ggcggcctcc
     1261 gaccagccat cagcactcag cggtcagcca tcagccaggc aggaagcccc tgcgccttgc
     1321 ccgagctgag agctgaccgc tgaaagtcga aagctattct actgcttgat cgagatgctg
     1381 aagccctcga tccgctcgtc ggaccgagcc gtccactgca accggcggct cacgccgtag
     1441 ttgtcgatct gctgatacag ggagatgcac ggcgcgtcct cgacaaagag cttgtcggcc
     1501 ttggagtaaa tctcctggcg tctcttggga ttcatattgg tctggccctc ttccaagagg
     1561 gaattgaagt ccgggttcca gtagttcgcc aggatctgcc ccgtccggaa catgggagta
     1621 taggtgtagt cggcgtccca ggtagtgttc ccccagccga tcaaccacat cggcccgccg
     1681 ttatgccggt aggccaggtt gttcatgtag gtcgtccact catgaacttt gagctgcgtc
     1741 cggatgccgg cctgggttaa ttgt
//
LOCUS       DBNGZC010000886         1419 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2423024, whole genome shotgun sequence.
ACCESSION   DBNGZC010000886 DBNGZC010000000
VERSION     DBNGZC010000886.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1419)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2423024"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..739
                     /locus_tag="AC16G0_15385"
     CDS             290..739
                     /locus_tag="AC16G0_15385"
                     /inference="COORDINATES: protein motif:HMM:NF025001.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR5101038.1"
                     /translation="MLLNQKGGVGKTSTCHHLAGTLAQGGKNILLVDNDPQASLTQGL
                     FGPQTTRDLDPRSTIAAVYSGPAVAEQIIHRTEISGIDLVPGSRCAMKYNNAEPWAAD
                     RDLQIGLRDVIETVRDRYDVVLIDCPPNLHLCSWAALVAGDHLLSLR"
     gene            complement(732..1340)
                     /locus_tag="AC16G0_15390"
                     /pseudo
     CDS             complement(732..1340)
                     /locus_tag="AC16G0_15390"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1313..1417)
                     /locus_tag="AC16G0_15395"
                     /pseudo
     CDS             complement(1313..1417)
                     /locus_tag="AC16G0_15395"
                     /inference="COORDINATES: protein motif:HMM:NF024980.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          259 a          464 c          450 g          246 t
ORIGIN      
        1 aggacgccta ccgccaggcg acaggtccga aggtgcgcaa tcgcggtgcg atgtttcaac
       61 atgtggcact cagacagcgt gaatagggtg gtggtagtca caaccaccac cgtccttcat
      121 cgggaaggga aaatgaggaa ctggatcgac tcggccgccg atagtgagct ggggccggtc
      181 cttcccgggg aagtgcgccg gccgcgggcc tgccggtccg gcgcggtcgg acgccctcac
      241 caaaaacttc cccgggaagt ggaactggca catgcccgcc gtctccatcg tgcttctgaa
      301 ccaaaaaggc ggggttggga agacctcgac ctgccaccac ttggcgggca cgctcgccca
      361 aggggggaag aatatcctct tggtcgacaa cgacccgcag gccagcctca cccaagggct
      421 gtttgggcca cagaccaccc gtgacctcga ccctagatcc accattgcgg cagtctactc
      481 gggccccgcc gtcgccgaac agatcatcca tcgcacggag atttccggga tcgacttggt
      541 acccggctcg cgatgcgcca tgaaatacaa caatgcggag ccatgggccg ccgaccgcga
      601 tctccagatc ggcctccgcg acgtcatcga gaccgtacgc gaccgctacg atgtggttct
      661 gatcgactgc ccaccgaatc tccacctatg ctcctgggcc gcgctggtcg ccggcgacca
      721 tctattgagc ctcaggtaag tcgctttaca cggagacccg catggcgaaa gaagctgagc
      781 aacagatcgg gtcgacgacg cagccgcgcg aatccccggt ccgcatgcgc gcggatctcc
      841 gactcggtct cgggtgtggc attgcgcatc cgctccttga cgttcccatg acaaccctcc
      901 tctgggttca gttccggggc gtacggcggg agccactcga catcgatctc ggggtgctcg
      961 gccaggaacg ccttgaccac ggcggaccga tgggcctgca agcggtcccc gatgatgatc
     1021 ttcggcccgc ggacttggcg accgagatgc tccaagtgtg cgacggtgtc ctcgccgtgg
     1081 atcgcgtgat cgaagtgctt cttgcagatt cggcccgacg cggtcagtcc gatggcggtc
     1141 gagacctggc gccttttgct gacgcggcgc agcaccggcg tttgcccctg gggagcccac
     1201 gtcgggccgg cccccaggtg gaacgagaac ccggtctcgt cgatgaagac gatcacggcg
     1261 ccgttgcggc gagccttttt ttgatgcgcg gccagtcccg atcgagccag gcccggatca
     1321 gttcctcgtc gcgctggcgg gcgtggacgg cgggcacttg aaccgtccaa cccaagtctc
     1381 tcagtcgggc cgagaggtag tgcgcatgat aagtcaggt
//
LOCUS       DBNGZC010000887         1699 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1542047, whole genome shotgun sequence.
ACCESSION   DBNGZC010000887 DBNGZC010000000
VERSION     DBNGZC010000887.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1542047"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..772)
                     /locus_tag="AC16G0_15400"
     CDS             complement(191..772)
                     /locus_tag="AC16G0_15400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101039.1"
                     /translation="MKSPLRWMLCGTTISLLSVTPLLGQHPQVRQGFWIGFGFGWGSL
                     GTSCTGCPSGDRQAGADGHLRLGGTLSPHVLLGGDIVGWTKSDSGQTVTAGNATFSAY
                     YYPMIESGLFVTGGFGGSTYAFSMNGNHATASGFGFSLGAGYDIRVARMFSITPIVHY
                     LWGSPGDLQYNGSTITPGLKTNIFDIGLNATFH"
     gene            complement(784..1362)
                     /locus_tag="AC16G0_15405"
     CDS             complement(784..1362)
                     /locus_tag="AC16G0_15405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101040.1"
                     /translation="MRRTSPVFVGVSLALIAAPPLPAQHPQVREGFWIAFGPGYASSH
                     FTCTGCTGETKSGGTFELRMGGTIRPDLLLGGDLSGWTHSENGVSETFGTAAFAGYFY
                     PMVQSGLFVKGGVGVSTAHFGVSGETAYGTGVGFLFGAGYDIRVGRNVSITPVVQFQY
                     GAVGNIKFPSGGTYFASLSQSLIGGGLDVTFH"
     gene            1454..1576
                     /locus_tag="AC16G0_15410"
     CDS             1454..1576
                     /locus_tag="AC16G0_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101041.1"
                     /translation="MSQPDIATYALTRRFHSLVAVDGLGVLVPVAARLFPEIVR"
BASE COUNT          330 a          563 c          538 g          268 t
ORIGIN      
        1 ggcgacgccg ctcgagcgac tcgttcccgt gcagccgtca cacgagtatc ccagggacga
       61 ccaaccggcc ccgaagccga tgaagaagcc tttcgggcga ggggcttcct gcgcggacac
      121 ccgcgccatg ccggccaggc cgaggctgac caccacggcg gataacacgg acgctctaat
      181 gcggctcctc tcaatggaat gtggcgttca aaccgatgtc gaaaatgttc gtcttcagcc
      241 cgggcgtgat cgtgctgccg ttgtattgca ggtctcccgg agagccccac aggtagtgaa
      301 cgatcggcgt gatcgagaac atgcgggcga cgcgaatgtc gtacccggcg ccgagcgaga
      361 acccgaagcc cgatgcggtg gcgtggttgc cgttcatgga gaacgcatag gtcgatccgc
      421 cgaagccccc ggtgacgaac aatcctgatt cgatcatggg atagtagtac gcactgaacg
      481 tcgcgtttcc agccgtgacc gtctgtccac tgtccgactt ggtccagccc acgatgtcgc
      541 cgccgagcag cacgtgagga ctcagggtcc cacccaggcg aaggtgtccg tcggctccgg
      601 cttggcgatc gccgctcgga caccccgtgc aggaagtgcc cagcgaaccc cagccgaagc
      661 cgaagccgat ccagaacccc tggcggacct gagggtgttg acccaagagt ggtgtaaccg
      721 acagcagcga aatcgtggtc ccgcacagca tccaacgcag cggcgatttc atgatctccc
      781 tcctcaatgg aacgtcacgt ccagtcctcc gccgatcagg ctctggctca agctggcgaa
      841 atacgtcccg ccgctcggga acttgatgtt gccgaccgca ccgtattgaa actgaacgac
      901 cggggtgatg gagacgttcc gtccgacccg gatgtcgtac cccgcgccga agaggaaccc
      961 caccccggtg ccgtaggccg tttccccact gaccccgaag tgcgcggtcg agacgcccac
     1021 gccgcctttg acgaagagtc cggattgcac catggggtag aagtaaccgg cgaatgcggc
     1081 cgtgccgaaa gtctcggaca caccattctc ggaatgcgtc caccccgaga ggtcgccccc
     1141 caggagcaga tcgggacgga tcgtgccgcc catccgcagt tcgaacgtcc ccccgctttt
     1201 tgtctccccg gtgcagccag tgcacgtgaa gtgggaggac gcatagcccg gcccgaacgc
     1261 gatccagaac ccctcacgca cctgcgggtg ctgggccggc aggggaggcg cggcgatcag
     1321 cgccagggag accccaacga acaccggtga agtccgccgc acagaatcct cctccgcgtt
     1381 ggccgcagta gctttcccgc acgtccgtca acacgttagg gagtcggccc atggaccgcc
     1441 agaggaatac ccgatgagcc agcccgacat tgcgacgtac gcgctcaccc gccggtttca
     1501 ttccctggtg gccgtggacg ggctcggggt gttagtgccg gtggcggccc ggctgtttcc
     1561 agaaatcgtg cgctgaggag gcgaatcgat ggatcaggaa catctgcatc gggtgctgga
     1621 ggagctgcac gcggagctgc gggcggcgaa gcccgtcgac gcggacagcc gcgacctgct
     1681 cgagcgcctg gaacgggac
//
LOCUS       DBNGZC010000888         2654 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1639445, whole genome shotgun sequence.
ACCESSION   DBNGZC010000888 DBNGZC010000000
VERSION     DBNGZC010000888.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1639445"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="AC16G0_15415"
     CDS             <1..468
                     /locus_tag="AC16G0_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012106985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HIR5101042.1"
                     /translation="ALVPLLPHGQGELARKLFAIGLFDAGFLGALCISLSTSWAVGEV
                     FGWAHSLNKGVKDAPWFYGVYLLMLLSAGAVVLIPGAPLITITMFVQVVAVTLLPSAL
                     VFLILILNSESFMGEHVNTRWQNIANWGIVIFVTVMSTLFAVSTLFPEWFGTT"
     gene            481..1761
                     /locus_tag="AC16G0_15420"
     CDS             481..1761
                     /locus_tag="AC16G0_15420"
                     /inference="COORDINATES: protein motif:HMM:NF015413.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter MgtE N-terminal
                     domain-containing protein"
                     /protein_id="HIR5101043.1"
                     /translation="MATPPTVGHKVADLPQGTGSFRFLYFSDLLKRPVCAGKIQDRRG
                     KLTDLVFALKEPYPEAAGIYLEHGWGKPTEFVPWERVLRIEDDAIFVTPPAQAGPYPP
                     FVDQAGWILLDHHLMGRTILDMDGRRIEVVNDVHLLEAKGHLLLVHVDTSFNGFLRRW
                     GLGRVRAIKEDLISWRYVQPLSVEDAVTTDKVSLSVTRRQLKELPSEDLADALEQLTG
                     SEQAALFSALDSEKAADTLMEAEPRAQRQLIANLRKERARQILGEMSVPQLANLFSVL
                     PHDDTTELMGLLPGESVERIRAILDEREAKAGELLSAEFVSFPKEATVGQVLDRVRRS
                     ELDPHHVSYIYVVAPEGSILQGVVDLRELLLARDEATLGDIMASPVVSAEEGNTQEDL
                     VQIFAKYQYRMIPVVDPQDRILGVIRYKDVMKTA"
     gene            complement(2020..>2654)
                     /locus_tag="AC16G0_15425"
     CDS             complement(2020..>2654)
                     /locus_tag="AC16G0_15425"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR5101044.1"
                     /translation="GGDIVFPHIESYLEDLEATRDGVLIEMERQAAERDFPIVGPLVG
                     RTICLLARSIGARRILELGSGFGYSAYWLARALPDDGELILTEMSETNSAQAKAFFRQ
                     GGVHCRVQVIQGDALDNIDRLPGEFDIIFNDVDKEQYPAAFHKGVPRVRRGGYFLSDN
                     MLWGGRVVDDPHHPTTQGVLELTRLLFQAPDLYTTILPIRDGLSVSLKLK"
BASE COUNT          429 a          862 c          866 g          497 t
ORIGIN      
        1 gccctggtcc cgctgcttcc ccacgggcag ggcgagctgg cccgaaagct ctttgccatc
       61 ggcctcttcg atgccgggtt cctgggggcc ctttgcatct ccctgtccac ttcctgggcc
      121 gtgggggagg tgttcggctg ggcccattcg ctcaacaagg gcgtgaagga tgccccctgg
      181 ttctacgggg tgtacctgct gatgctgctc agcgccgggg cggtcgtcct gatcccgggg
      241 gcgccgctca tcaccatcac catgttcgtc caggtcgtgg cggtcacgct gctgccgtct
      301 gccctggtgt tcctcatcct catcctgaac tccgagtcct tcatgggtga acatgtcaac
      361 acccgctggc agaacatcgc caactggggc atcgtgatct tcgtcacggt gatgtccacc
      421 ctgtttgcgg tcagcaccct cttcccggag tggttcggaa cgacatgaca ggaggcggct
      481 atggccacgc cacccaccgt gggccacaag gttgcggacc tgccccaggg gaccggcagc
      541 ttccgatttc tgtacttttc cgatctcctg aagcggccgg tgtgcgcggg caagatccag
      601 gatcgccggg ggaagctcac ggacctggtg ttcgcgctca aggagcccta tcccgaggcg
      661 gcggggattt atctggagca cggctggggg aagccaacgg agttcgtccc ctgggagcgg
      721 gtgcttcgca tcgaggacga tgcgatcttc gtgacacccc ccgcgcaggc aggcccctac
      781 cccccgttcg tggaccaggc cggctggatc ctgctggacc atcatctcat gggacggacg
      841 atcctggaca tggacggccg gcgcatcgag gtggtcaacg acgttcacct gctcgaggcc
      901 aagggccacc tgctgctggt ccatgtggat acgtccttca acgggttcct ccggcgctgg
      961 gggctcggcc gagtccgcgc catcaaggag gacctgatat cctggaggta cgtccagccc
     1021 ctctccgtgg aggatgctgt caccacggac aaggtctccc tctcggtgac gcggcggcag
     1081 ctcaaggaat tgcccagcga agacctggcg gacgccctgg agcaactcac ggggagcgag
     1141 caggcggccc tcttctccgc cctggattcc gagaaggccg cggacacgct catggaggcg
     1201 gagccgcggg cccagcgcca gctcatcgcg aacctccgga aggagcgcgc ccgtcagatt
     1261 ctcggcgaga tgtccgtccc tcaactggcg aacctcttct cggtgctccc ccacgacgac
     1321 accacggagc tgatggggct cctgccgggg gagagcgttg agcgcatccg ggctattctg
     1381 gacgaacgcg aggccaaggc gggggagctc ctgtccgcgg agttcgtctc cttccccaaa
     1441 gaggcaacag tcggccaggt gttggaccgc gtgcgccgct cggagctgga tccgcaccac
     1501 gtctcctaca tctacgtggt ggcgccggag gggagcattc tccagggtgt ggtggacctg
     1561 cgcgagctcc tcctggcgag ggacgaggcc accctgggag acatcatggc ctcgccggtg
     1621 gtctccgccg aggagggaaa tacgcaggag gacctggttc agatcttcgc caagtaccag
     1681 taccgcatga ttccggtggt ggaccctcag gatcgcattc tcggtgtcat ccgctacaag
     1741 gacgtcatga agaccgcgta acccagatca cgggttgagg agcctctgat gccacgcgtt
     1801 ccggtgaccc ccctggtgcg ttttgcgctg tacttccttc gggcctatct gatcctgctt
     1861 ctgagtctcc tgtgcctccg gtttctgcaa gtcttccggt agcgtggatg ctcaaacgcc
     1921 ggaccttcct tccccgttgt tgacgcggtg gagagctgga ggggggcgtc aagaggtttc
     1981 ccatggtgga gagcccgctc cgggcggaga cccttcgccc tacttcagct tcaagctcac
     2041 ggacagcccg tctcggatgg gaaggatcgt ggtgtagagg tcgggcgcct ggaacaaaag
     2101 gcgagtcagc tccagcaccc cctgggtggt cgggtgatgg ggatcgtcca ccacgcggcc
     2161 gccccagagc atattgtcgc tcaggaagta cccgccgcgg cggacccgcg ggacgccctt
     2221 gtggaacgcc gcggggtatt gttccttgtc cacatcgttg aagataatgt cgaactcgcc
     2281 cggcagccgg tcgatgttgt ccagggcgtc gccctggatg acctggactc gacaatggac
     2341 gccgccttgg cggaagaacg ccttggcctg ggcgctgttt gtttcggaca tctcggtgag
     2401 gatgagctcc ccgtcgtcgg gcagcgcgcg cgcaagccag taggcggagt agccgaaccc
     2461 ggacccaagc tccaggatcc gccgggcgcc gatgctccgg gccaggagac agatggtccg
     2521 ccccaccagg ggacccacga tggggaagtc gcgctcggcc gcctgccgct ccatctcgat
     2581 cagcacgccg tcccgggtgg cttccaggtc ctcgagatag ctctcgatgt gagggaacac
     2641 aatatccccg ccgc
//
LOCUS       DBNGZC010000889         3281 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1251857, whole genome shotgun sequence.
ACCESSION   DBNGZC010000889 DBNGZC010000000
VERSION     DBNGZC010000889.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1251857"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3148)
                     /gene="carB"
                     /locus_tag="AC16G0_15430"
     CDS             complement(<1..3148)
                     /gene="carB"
                     /locus_tag="AC16G0_15430"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR5101045.1"
                     /translation="MPRRDDIRKILIIGSGPIIIGQACEFDYSGTQACKALRRLGYDI
                     VLVNSNPATIMTDPTMADVTYIEPLNTRALTEIIERERPDALLPNLGGQTGLNLSSAL
                     ARDGVLEKYGVRVIGVNVDAIRRGEDRQEFKTTMTRLGLEMPRSDIAHSLAEALEVVR
                     RIGVPVVIRPAYTLGGTGGGFAYNLEEFETIVSRGLSMSLVGQVLVEESVLGWEELEL
                     EVVRDARGQKITVCFIENVDAMGVHTGDSFCTAPMLTIAPDLQRRLQRHSYDIVDAIE
                     VIGGTNIQFAHDPKTGRVVVIEINPRTSRSSALASKATGFPIAFVSSLLACGLTLDEI
                     PYWRDGTLDKYTPSGDYVVVKFARWAFEKFKGVQDKLGTQMRAVGEVMSIGKTYKEAL
                     QKAIRSLEIGRSGLGFARDFHRRSLAELLDLLDEPSSERQFILYEALRKGAGTDALHA
                     RTHIKAWFIDQMRELVELEERLLAHKGHMPPEALLTQAKRDGFADKYLAQILHLREAD
                     IRARRTELGVTEAWDAVPVSGVENAAYYYSTYNAPDRVPASRRRKVLVLGGGPNRIGQ
                     GIEFDYCCVHAAIALRQAGYESIMINCNPETVSTDYDTSDKLYFEPLTVEDVLAIYVK
                     ERPEGVIVQFGGQTPLNIAAELEAAGVRILGTSPAVIDLAEDRDRFRRVIQRLQIPQT
                     ESGMASTLEEALLIAKRIGYPLMVRPSYVLGGRAMEKVHDEEMLREYVAKAVEISPER
                     PILIDRFLENAIEAEADAIADGRDAFVPAVMEHIELAGVHSGDSACVIPPVSIPRKHL
                     DTIEEYTRKIAVEFGVVGLLNVQYAIAADTVYILEANPRASRTVPLVSKVCNIAMARL
                     ATQAMLGQKLSDLNLTRRPIPHFGVKEAVFPFNMFPEVDPVLGPEMRSTGEVLGMADS
                     YGEAFFKAEEAAGQLLPLSGTVLITVAEHDAGALEAARRFAELGYRIIATSGTRLFLE
                     ENGVAAQPILKLHEGRPNIADAIQNGEIQLVVNTPAGKLSAYDDSYIRKAAIKYKVPY
                     VTTTTAAIATARGVAAVRQ"
BASE COUNT          504 a         1087 c         1117 g          573 t
ORIGIN      
        1 cctggcggac cgcggcgact ccccgggcgg tggcgatcgc ggcggtggtc gtggtgacgt
       61 aggggacctt gtacttgatg gctgccttgc ggatgtagga gtcgtcgtag gcgctcagct
      121 tgccggcggg ggtgttgacc accagctgga tctcgccgtt ctggatggcg tcggcgatgt
      181 tcgggcgacc ctcgtgcaat ttgaggatgg gctgcgccgc gaccccgttc tcttccagga
      241 acagacgtgt gccgctggtg gcgatgatcc ggtagcccag ctcggcaaag cgccgcgcgg
      301 cctccagggc gcccgcatcg tgttccgcca cggtgatgag gaccgtcccc gaaaggggca
      361 acaactgccc cgccgcctcc tccgccttga agaaggcctc gccgtaactg tccgccatcc
      421 ccaggacctc gccggtcgac cgcatctcgg gccccagcac gggatccacc tccgggaaca
      481 tgttgaaggg gaacaccgcc tccttgacgc cgaaatgcgg gatcggccgc cgtgtcaggt
      541 tcaggtcgga gagcttctgg cccagcatcg cctgggtggc cagccgcgcc atcgcgatgt
      601 tgcagacctt gctgaccagc ggcacggtcc ggctggcgcg gggattggcc tccaagatgt
      661 aaacggtgtc ggcggcgatg gcgtactgga cgttcagcag tcccaccacc ccgaactcca
      721 cggcgatctt ccgcgtgtac tcctcgatgg tgtccaggtg cttgcgcggg atgctgaccg
      781 gcgggatcac gcaggcggag tcgcccgagt ggacgcccgc cagctcgatg tgctccatca
      841 ccgccgggac gaaggcgtcg cggccgtcgg cgatggcatc cgcctcggcc tcgatggcat
      901 tctccaggaa gcggtcgatg aggatgggcc gctccgggga gatctccacg gccttggcca
      961 cgtactcccg cagcatctcc tcgtcgtgga ctttctccat ggcgcggccg cccagaacat
     1021 aggaggggcg gaccatcagc ggatagccga tccgcttcgc gatgaggagc gcctcctcca
     1081 gcgtgctggc catgcccgac tccgtctggg ggatctggag gcgctggatg acgcggcgaa
     1141 agcggtcgcg gtcctcggcg aggtcgatga cggcgggcga ggtgccgagg atccgcacgc
     1201 ctgcggcttc cagctccgcg gcgatgttga gcggggtctg cccgccgaac tggacgatga
     1261 ccccttcagg ccgctccttg acgtagatcg ccaggacatc ctcgacggtg agcggctcga
     1321 agtacagctt gtccgaggtg tcgtagtcgg tggagacggt ctcggggttg cagttgatca
     1381 tgatcgactc gtagcccgcc tggcgcagcg cgatggccgc gtgcacgcag cagtagtcga
     1441 actcgatccc ctgcccgatc cggttggggc cgccgcccag caccaggacc ttgcgccgcc
     1501 ggctggccgg aacccggtcg ggagcgttgt acgtcgagta gtagtaggct gcgttctcga
     1561 ccccgctgac cggcacggca tcccacgcct cggtcacgcc cagctccgtg cgccgggcgc
     1621 ggatgtccgc ctcgcggaga tggagtatct gggccaggta cttgtccgcg aacccgtccc
     1681 gcttggcctg ggtgaggagt gcctcgggcg gcatgtggcc cttgtgcgcc agcaggcgct
     1741 cctccagctc caccagctcc cgcatctgat cgatgaacca cgccttgatg tgggtgcggg
     1801 cgtgcagggc gtccgtcccg gcccccttgc gcagggcctc gtacaggatg aactgccgct
     1861 cgctggaggg ctcgtccagc aggtccagga gttccgccag cgacctgcgg tgaaagtccc
     1921 tggcgaaccc gaggccggag cggccgatct ccaaggagcg gatcgccttc tgcagcgcct
     1981 ccttgtaggt cttgccgatg ctcatcacct cgccgacggc ccgcatctgg gtgccgagct
     2041 tgtcctgcac ccccttgaac ttctcgaagg cccagcgggc gaacttgacc accacgtagt
     2101 cgcccgaggg cgtgtacttg tccagggtgc cgtcccgcca gtaggggatc tcatccagcg
     2161 tcaggccgca ggccaggagg gacgagacga acgcgatggg aaagccggtg gccttggacg
     2221 ccagggccga ggagcgggag gtgcgggggt tgatctcgat caccacgacc cggccggtct
     2281 tcgggtcgtg ggcgaactgg atgttggtgc cgccgatcac ctcgatggcg tccacgatgt
     2341 cgtaggagtg ccgctgcagg cgccgctgga gatccggtgc gatggtcagc atgggagcgg
     2401 tgcagaagga gtcgcccgtg tgcacgccca tggcgtccac attctcgatg aagcagaccg
     2461 tgatcttctg cccccgggcg tcgcgcacga cctccagctc cagctcttcc cagccgagca
     2521 ccgactcttc caccagaacc tggccgacca ggctcatgga cagcccgcgg ctcacgatgg
     2581 tctcgaactc ttccaggttg taggcaaaac cgccgcccgt cccgcccaag gtataggcgg
     2641 gacggatgac gaccggaacg ccgatccgcc tgacgacctc cagcgcctcc gccaggctgt
     2701 gggcgatgtc gctccgcggc atctccaggc cgagacgcgt catggtggtc ttgaattcct
     2761 gccgatcctc cccccgccgg atggcatcca cgttgacgcc gattacccgg acgccgtact
     2821 tctccaggac cccgtctcgg gccagggcgg aggagaggtt gagcccggtc tggcctccca
     2881 gattcggcag caaggcgtcg ggacgttccc gctcgatgat ctcggtcagg gcccgcgtgt
     2941 tgagcggctc gatgtaggtg acgtcggcca tggtcgggtc ggtcatgatg gtcgcgggat
     3001 tggagttgac caggacgata tcgtagccca gccgtctgag cgccttgcag gcctgcgtgc
     3061 cggagtagtc gaactcgcag gcctggccga tgatgatcgg gccggagccg atgatcagga
     3121 tcttccggat gtcgtcacgc ctgggcatgt tcacccctca aattgactgc tggaagccgg
     3181 agtcgcgccc ttctcgagcg ggcagatcat ggcccacccg gcagaacccc ggcatctggc
     3241 tgggccgatc ttgacggtac tcctgctgcg aaaaggccgg g
//
LOCUS       DBNGZC010000890         3965 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_174537, whole genome shotgun sequence.
ACCESSION   DBNGZC010000890 DBNGZC010000000
VERSION     DBNGZC010000890.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_174537"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="AC16G0_15435"
     CDS             <1..990
                     /locus_tag="AC16G0_15435"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF017555.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR5101046.1"
                     /translation="ACAERGAQILLEEIRSDLRAFGITFDTWFSEKSLFESGEVGAAL
                     ATLRAGGYLYEEGEAIGFRASEFGDEKDRILIRGTGEPTYFTSDIAYHANKIHRGFVR
                     LINLWGADHGGYIPRVKAAIHALGHDPDILHVILLQMVRVLRGGVPVPMSKRSGQFVE
                     LREVVSEVGKDAARFIFLTRRSESQLDFDIEVAKRQSMDNPVFYVQYAHARACSMARK
                     AAEAGIGGPYTDADLSKLMLPEELAIMKHLAVYPELVRSAALAHEPHRVTTYLQELAA
                     SFHGYFTRYKDSEERVISGDRDLSRARLAMVAAVRQVVRNGLAILGVSAPERM"
     gene            1081..1911
                     /locus_tag="AC16G0_15440"
     CDS             1081..1911
                     /locus_tag="AC16G0_15440"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR5101047.1"
                     /translation="MTDMRQHLRAMFADVRRFLVTDGLQKRGIVQQNPHGDVTKEFDY
                     LAEGRIIDYCAREIPQPVRILTEERGEIRTRSGAAAWTVIVDPVDGSENFARGNEMSS
                     VSLAALPGEDVPRPEDVVVALVGRIFSGTTYEGEKGGGAWRNGQPIRASAVTSLSDAL
                     VSIDFNFRGKATVARLFPLLATIPDARRFGTAAAEFVAVAHGGADAYVDARDTLSPEN
                     YMAAFLIVAEAGGVISDRFGKPLAPIRSMTQGQSIVAAATPALHKAILTVLGEDAATR
                     "
     gene            2224..3615
                     /gene="lpdA"
                     /locus_tag="AC16G0_15445"
     CDS             2224..3615
                     /gene="lpdA"
                     /locus_tag="AC16G0_15445"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR5101048.1"
                     /translation="MSAYDVVILGAGPGGYVAALRAAQLGATVCLIENDVVGGTCLNR
                     GCIPSKALIHSAALWKRALEGTAFGVTASNWAFDWAAAQTRKSQIVGTQVKGVHMLLS
                     TAKVAVKQGVGSLLDARSVKITANGSEETVTAKAIIIASGSEPRGLTGVEIDGERVLT
                     STEALQLPALPKTFLIVGGGVIGMEFASMLSSLGTQVTVVEMLPQILPTEDPMLVRVL
                     QGALQKQGVAFHVNTKVEKVEHTGAGVRVQISGGTSVEAERVLIATGRSLNSAGIGVE
                     KAGVMLAHGAIQVNERMETSARGVYAIGDVTGISLLAHVASMQGLVAAANATGGQAVM
                     DYSAVPNCIYTDPEIASVGLSEPQAKTQGREVKVGRFNFASLGKAMCIGETAGMVKLV
                     ADVQTDKVLGVGIVGPHATDIIAEGVLAVRHGLTVAQVAEAIHAHPTLPEAMGEAAHD
                     VHGHAIHKARLRA"
     gene            3652..>3965
                     /locus_tag="AC16G0_15450"
     CDS             3652..>3965
                     /locus_tag="AC16G0_15450"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525284.1"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIR5101049.1"
                     /translation="MEILQIEGTATPAPRRLPPWLRAKAGHWERVHDIKVLLQGSRLH
                     TVCEEARCPNQGECWTRGTATFMLLGDTCTRSCGFCAVATGRPGAPDPLEPRHVAEVS
                     RRL"
BASE COUNT          695 a         1229 c         1408 g          633 t
ORIGIN      
        1 gcctgcgccg agcgcggggc ccaaatcctg ctggaggaaa tccgctcgga tctccgggcc
       61 ttcggcatca ccttcgatac ctggttctcg gagaagagcc tgttcgagag cggcgaggtg
      121 ggggccgctc tggccacgct tcgcgccggc ggctacctct acgaggaggg cgaggcgatc
      181 ggcttccgcg ccagcgagtt cggtgacgaa aaggaccgca tcctgatccg gggcacgggg
      241 gagccgacct acttcacgtc ggacatcgcc tatcacgcca ataagatcca tcgcggcttc
      301 gtccgcctca tcaatctctg gggggcggac cacggaggat acatcccccg cgtgaaggcg
      361 gctattcatg ccctcgggca cgacccggac attctccacg tgattctact ccagatggtg
      421 cgtgtgctgc gcgggggcgt gccggtaccc atgtccaagc gcagcggcca gttcgtcgag
      481 ttacgcgagg tggttagcga ggtgggaaag gatgcggcgc ggttcatttt cctcacgcgg
      541 cgctccgaat cacagctcga cttcgacatc gaggtggcca agcgtcagag catggacaac
      601 ccggtctttt acgtgcagta cgcccacgct cgggcctgca gcatggcccg gaaggcagcc
      661 gaggcgggca tcggcggacc ctataccgat gccgatctct ccaagctgat gctcccggaa
      721 gagctggcca tcatgaagca tctggcggtc tatccggagt tggtgcggag cgcggcgctg
      781 gctcacgagc cgcatcgagt gaccacgtat ctccaggaac tggccgccag cttccacggc
      841 tacttcacac gctacaagga cagcgaggag cgcgtcatct ccggcgaccg cgacctcagc
      901 cgcgccaggc tcgccatggt cgccgccgtg cggcaggtcg tgcggaacgg cctggccatc
      961 cttggggtct ccgcccccga gcggatgtga cgcctgcctc acgcagaggc gcagagaaaa
     1021 ttggacatct gcgcaatccg cgtaatctgc ggataaattg aagtcagaaa cggatcgcaa
     1081 atgactgaca tgcggcagca cctgcgggca atgttcgccg acgtccggcg gtttctcgtg
     1141 acggatggac tccagaagcg cggtatcgtg cagcagaacc cccacggcga cgtgaccaag
     1201 gagttcgatt atctggccga gggtcgcatc attgactact gcgcccgcga gatcccgcag
     1261 ccggtccgga tcctgacgga ggagcgaggc gagatccgca cccggtcagg ggccgcggcc
     1321 tggaccgtga tcgtggaccc ggtggacgga tcggagaact tcgcccgggg aaacgagatg
     1381 tcctccgtat ctctggccgc gcttcccggt gaggacgtcc cgcggcccga ggatgtcgtc
     1441 gtcgccttgg tgggccggat cttcagcggg acgacgtacg agggggagaa gggtggcgga
     1501 gcctggcgga acggccagcc gatccgagcc tccgccgtga ccagcttgtc cgatgccctc
     1561 gtctcaatcg acttcaactt ccgggggaag gcgacggtgg ccaggctgtt ccccctcctg
     1621 gccaccatcc cggacgcccg gcggttcggg acggcggccg ccgagttcgt ggcggtggcc
     1681 cacggcggtg cggatgcgta cgtggacgct cgggacacgc tgagcccaga gaactacatg
     1741 gctgcattcc tcatcgtcgc cgaggccggg ggcgtgatct cggaccgctt cggcaagccg
     1801 ctggccccga tccggtccat gacccagggc cagagcatcg tcgccgccgc cacgcccgct
     1861 ctgcacaagg cgatcctgac cgttctgggc gaggacgctg ccacgaggta atgcagacct
     1921 ttcccctagg cgaccaagta cacgttgaca cccctgggtt cgtttggtag cctctggaat
     1981 gcaacgtcgc gaggcagaag ggttcgacgc ggtggggaag tggagatccc cggtccggct
     2041 gcggttgata gattccagcg gcagcccggt aattcgtcgg ccatcgtcaa gcccccggtt
     2101 gggggcttcg ttgttcttgg ggggagagcg agagccgagc gccgagagcc aagcggtgaa
     2161 agctgagagc cgaaagcttc gagacgagcc ggattgcagt ggaggaagag gagcctgaga
     2221 cgtatgagcg cgtatgatgt ggtgatcctg ggagctggac cgggggggta tgtggcggct
     2281 ctgcgggcgg cccagttggg ggcgaccgtc tgtctcatcg agaatgacgt ggtgggaggc
     2341 acctgcctga accgcggctg catcccgtcc aaggccctca tccattctgc ggcgctgtgg
     2401 aagcgggcgc tggagggcac cgcctttggc gtcactgcga gcaactgggc cttcgactgg
     2461 gccgccgccc agacgcggaa gagccagatc gtcggcaccc aggtgaaggg tgtccacatg
     2521 ctcctgagca cggccaaggt ggcagtgaag cagggggtgg ggagtctcct ggacgcccgg
     2581 agcgtgaaga tcaccgccaa cggatcggaa gagaccgtga cggccaaggc catcatcatc
     2641 gcctcgggct cagaacccag gggcctgact ggggtagaga ttgacgggga acgggtgctc
     2701 accagcaccg aggcccttca gctccccgcc ctgccgaaga cctttctcat cgtgggcggt
     2761 ggtgtgatcg ggatggagtt cgcgtccatg ctgagcagtc tcggcaccca ggtgacggtg
     2821 gtggagatgc tgccgcagat tcttccgacg gaggatccga tgctggtgcg ggtcctccag
     2881 ggggcgctcc aaaagcaggg cgtcgccttc cacgtgaata ccaaggtgga gaaggtggag
     2941 cacaccgggg ccggcgtccg cgtgcagatc agcggcggga cgagtgtgga ggcggagcgt
     3001 gtgctgatcg cgaccggtcg gagcctgaac agcgccggca ttggcgtcga gaaggcaggg
     3061 gtcatgttgg cccacggggc catccaggtg aacgagcgga tggagacctc ggcgcgcggc
     3121 gtgtacgcca tcggcgacgt gacgggcatc tctcttctcg cccacgtggc ctcgatgcag
     3181 gggctggtgg ccgcagcgaa tgccacgggc ggacaggccg tcatggatta ttcggccgtc
     3241 ccgaactgca tctacaccga tcccgagatt gccagcgtgg gcctgtccga gccccaggcc
     3301 aagacgcagg ggcgggaggt gaaggtcggc cgcttcaact tcgcctcgct gggcaaggcc
     3361 atgtgcatcg gcgagacggc cggcatggtg aagctcgtgg ccgacgtcca gaccgacaag
     3421 gtcctggggg tcgggatcgt cggaccgcac gccaccgaca tcatcgcgga gggcgtcctg
     3481 gccgtgcgcc atggactcac ggtggcccag gtggctgagg ccatccacgc gcacccgacg
     3541 ctgcccgagg ctatgggtga ggccgcccac gatgtgcacg gccatgccat ccacaaggcg
     3601 aggctgagag cctagagtcg agagccgagc gccgagagtc gagagacgcg aatggagatt
     3661 ttgcagatcg agggaacggc gacaccggcg cccaggaggc tgccgccgtg gctgcgcgcc
     3721 aaggcgggcc actgggagcg ggttcacgac ataaaggtcc tgctccaggg gtctcgcctg
     3781 cacacggtat gcgaggaggc tcgctgcccc aaccaggggg aatgctggac gcgggggacc
     3841 gccaccttca tgctgctggg cgacacctgc acccggagtt gcggcttctg cgccgtggcc
     3901 accgggcgcc cgggggcgcc ggacccgctg gagccccggc atgtggccga ggtgagccgg
     3961 cggct
//
LOCUS       DBNGZC010000891         2548 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_174634, whole genome shotgun sequence.
ACCESSION   DBNGZC010000891 DBNGZC010000000
VERSION     DBNGZC010000891.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_174634"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="AC16G0_15455"
     CDS             <1..278
                     /locus_tag="AC16G0_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010152186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR5101050.1"
                     /translation="ELFNQPRHPYTAALMAAVPVADPRVDSGPGERKGEVPSPANPPS
                     GCYFHPRCAYAADACRTQPPDFREISPGHFVACHRSEEIKLPGISLE"
     gene            1208..1462
                     /locus_tag="AC16G0_15460"
     CDS             1208..1462
                     /locus_tag="AC16G0_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR5101051.1"
                     /translation="MLDLIWIVVIGAVVGALAKFIMPGADPGGFFVTALLGMGGALVA
                     TFLGRFVGFYGPGQGAGFLASVIGAILVLIIYRSVRKRQV"
     gene            1507..1995
                     /locus_tag="AC16G0_15465"
     CDS             1507..1995
                     /locus_tag="AC16G0_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101052.1"
                     /translation="MGILDELLGGGQRQRDFTDFVKRYEQGHPAEGYSDQEVADRYRE
                     VAHAVPSDQYAQAVQEALARLSPEERAGFLKMLQERAQTRGVALPGSTASDPKDLGQV
                     VTELHETPGRLRDILGGGSAQPQTAGSSPLAGMFASPLAKAALAGIAAMIVKRVMQGP
                     TR"
     gene            2096..>2548
                     /locus_tag="AC16G0_15470"
     CDS             2096..>2548
                     /locus_tag="AC16G0_15470"
                     /inference="COORDINATES: protein motif:HMM:NF024245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HIR5101053.1"
                     /translation="MPKLNVVLAGGLLVAALALGLIAATPNDVAAQAAKSAASAAKVD
                     INSATQAELEKLPGVGEATAKKIVAGRPYQAVGDLSKAGVPKNTIEKITPLVTVGPAA
                     AAKAATPSPAAPTAPAGTTKSATPEKTKPAPSVAKAAPSKGMVWVNTAT"
BASE COUNT          492 a          773 c          871 g          412 t
ORIGIN      
        1 gcgagctgtt caaccagccc cgacatccct atacggccgc cctgatggcg gccgtgccgg
       61 tggccgaccc gcgggtggac tcgggccctg gcgaacgcaa gggagaggtc cccagccccg
      121 ccaatcctcc gtcaggctgc tattttcacc cgcgctgcgc ctatgcggcg gatgcttgcc
      181 gcacacagcc acccgacttc cgcgagatca gcccggggca cttcgtcgcc tgccatcgct
      241 ccgaagagat caagctgccc ggaataagcc tcgaataatt cagtaggact tgccccgaga
      301 gcgttaaacc cggaaatctc gtcgccaaga tcgccaagat cgcaaagact ggccagccat
      361 ctatccataa gagcattgcg gtgaaatctt tcgggcggtc tatcccggtg attttccatc
      421 cgcgggttga attcgcgaag ttcctttggg gtctttgcgc accaacggcg aggctgtcga
      481 tacccgcggt tgaagtgcac tacgcactac ttactatgcg gactctaatg aaggcgacag
      541 tccacctcca aaaccgcaga gtccggggga gagtccgagg aggggtggcg gcccacccct
      601 ctcggcgggt acccgcgccg caggcgtggg tgcgcgggga gggcgagcgg ttgcccgtca
      661 cgatgcaagc gcgcgggcct tccccgcgaa ctacgccgtc cgggcggata tgtagcctta
      721 cttccggacc gcttagtagg tcagaagttc ggtcagggga aaatgaacct tgacaattgg
      781 cattcccttt gactacgttc cgagatggat tcgcaatcag gcaggacaga ctcaccgaga
      841 agacgcgaag agtccggcgt gctggtcgtg ggagggagag aggagggcaa aacctgcaca
      901 tcacaacaag ctcgcctgcc gtcgtgctgt tggcgatcct gggagtctgc ggctgcgcaa
      961 tgatccggaa gaatccgaag actgcgttcg gggcgggccc cggggcctga caaggaggtg
     1021 tgcgatattc gaggagctca aggagttgtc ttgggggccg agggacaacc tatagcgatc
     1081 taggagcgat gggctaggcc tccgccgggg gcgcgccgcg gccgagggcc ggaccattgc
     1141 ggagccccca gaagcacatt ccgattacgc accgactgtt accgctgaaa agccggagga
     1201 gaacaagatg ctcgacctca tctggatagt cgtgatcggt gcggtggtcg gcgccctcgc
     1261 caagttcatc atgccggggg cagatccggg aggcttcttc gtgacggcac tgctcggcat
     1321 gggaggggca ctcgtggcca ccttcctggg tcgatttgtc gggttctacg gacccgggca
     1381 gggcgcaggc ttcctcgcgt cggtcatcgg tgcaatcctg gtgttgatca tctaccgcag
     1441 cgtcagaaag cgtcaggtct agggggcgga cgttcggcgg tacaagtgca cagagaggag
     1501 tgcaggatgg gcattctcga tgagctgctt ggcggtggac aacggcagcg ggacttcacg
     1561 gacttcgtga agcggtacga gcagggacac cccgcagagg gttactcgga ccaggaggtc
     1621 gccgacaggt atcgcgaggt ggcgcacgcc gtcccctcgg accagtacgc gcaggccgtc
     1681 caggaggcgc tcgcgcggtt gtcgccggag gagcgtgcgg ggttcctcaa aatgctgcag
     1741 gaacgcgcac agacccgtgg ggtcgccctg cccgggtcga cggcatcgga tcccaaggac
     1801 ctggggcagg tagtcaccga gctacacgag acgccgggcc ggctccggga catcctcggg
     1861 ggcgggagcg cccaacctca gacggccggg tccagtccgc tggcgggcat gttcgcgtcg
     1921 ccgttggcca aggcggcgct ggcgggaatc gccgccatga tcgtcaagcg agtgatgcag
     1981 gggccaaccc gctagcgtca tcccggacgt caggaaggtg ggcttacaac gcgcctgagg
     2041 atggggttcg cgctagaccg tccggcgtaa ccgagaaggc aaaaaggaga aaagaatgcc
     2101 aaagctcaac gtcgtccttg ccggtggttt gcttgtggct gcgctggcgc tcggactgat
     2161 tgcggctact cccaacgacg tcgccgccca ggcggcgaag agcgcggctt cggccgcaaa
     2221 ggtggacatc aactccgcaa cccaggcgga attggagaag ttgccggggg tcggggaggc
     2281 gactgccaag aagatcgttg ccgggcgccc ctatcaggcg gtcggcgatc tgtctaaggc
     2341 cggtgtgccg aagaatacga tcgagaagat cacgcctctc gtgacggtgg gacccgccgc
     2401 ggccgccaag gcggcgactc catcgccggc cgcgccgacg gcgcccgccg ggaccacgaa
     2461 aagcgccacc ccggaaaaga cgaagcccgc gccgagcgtg gccaaggcag ctccctccaa
     2521 gggcatggtc tgggtgaaca cggccacc
//
LOCUS       DBNGZC010000892         1289 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1146971, whole genome shotgun sequence.
ACCESSION   DBNGZC010000892 DBNGZC010000000
VERSION     DBNGZC010000892.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1146971"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="AC16G0_15475"
     CDS             complement(<1..800)
                     /locus_tag="AC16G0_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HIR5101054.1"
                     /translation="MANALSAKEQTLVDAYWRAANYLSVGQIYLYDNPLLKKPLTKEH
                     IKPRLLGHWGTTPGLNFIYVHLNRVIRKHDLDMIYITGPGHGGPGLVANAYLEATYTE
                     VYPNISQDEEGMKRLFTQFSFPGGIPSHVAPETPGSIHEGGELGYAVSHAYGAAFDNP
                     DLIVACVVGDGEAETGPLATSWHSNKFLNPVTDGVVLPILHLNGYKIANPCVLARISH
                     EELDHLFRGYGYIPHFVEGHEPGEMHQKMAATLDAVIGEIQKIKADAKR"
     gene            complement(855..>1289)
                     /locus_tag="AC16G0_15480"
     CDS             complement(855..>1289)
                     /locus_tag="AC16G0_15480"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006911887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HIR5101055.1"
                     /translation="SLETMLLKPNMVIPGKTCLRPASVQDVATATLGCLRRNVPAAVP
                     GIVFLSGGQSDRMATAHLNAINRLPESRPWTITFSYGRALQDPALEAWHGQDANLKAG
                     QEALYCRARCNGAASLGMHTEDMETASAGAGSPAHRREWHDD"
BASE COUNT          189 a          416 c          440 g          244 t
ORIGIN      
        1 cgcttcgcgt cggccttgat cttctggatc tcaccgatca cggcgtccag cgtggccgcc
       61 atcttctggt gcatctcacc cggctcgtgc ccctccacga aatgggggat gtagccgtac
      121 ccgcggaaga ggtgatccag ctcctcgtgg ctgatccggg caaggacgca cggattggcg
      181 atcttgtagc cgttcaggtg gaggatcggc agcacgaccc cgtcggtcac cgggttcagg
      241 aacttgttgg agtgccagct cgtcgccagg ggtccggtct ccgcctcgcc gtcgcccacg
      301 acgcaggcca cgatcaggtc ggggttgtcg aaggcggccc cgtacgcgtg cgagacggca
      361 tagccgagct caccgccctc gtggatcgag ccgggggtct ccggggccac gtggctcgga
      421 atgccgccag gaaacgagaa ctgggtgaag agccgcttca tgccctcctc gtcctgggag
      481 atgttcggat agacctcggt gtaggtcgcc tcgaggtagg cgttcgcgac caggccgggc
      541 ccgccgtgcc cggggccggt gatatagatc atgtcgaggt cgtgctttct gatcacccgg
      601 ttcaggtgga cgtagatgaa gttgagtccg ggcgtggtcc cccagtggcc gaggagccgg
      661 ggcttgatgt gctccttggt cagcggcttc ttcagcagtg gattgtcgta gaggtagatc
      721 tggccgaccg acaggtaatt ggcggcccgc cagtatgcgt caacgagtgt ctgttccttg
      781 gccgacagcg cattcgccat gactgcctcc tctggtcccg ggttgcccgc cgtgctggcg
      841 gcaggccgcg cgtctcagtc gtcgtgccac tcgcgacgat gcgccggact gccggcaccc
      901 gccgatgccg tttccatgtc ctccgtgtgc atcccgagac tggccgcccc gttgcaccgg
      961 gcccggcagt acagggcctc ttgaccggcc ttcagattcg cgtcctgtcc gtgccacgcc
     1021 tcgagcgccg gatcctggag cgcgcgcccg taggagaagg tgatcgtcca gggcctggac
     1081 tcgggcaatc ggttgatggc gttcaggtgg gcggtggcca tgcggtcact ctggccgccc
     1141 gacaggaaca cgatcccggg caccgccgcc ggcacattcc ggcgcaggca acccagcgtg
     1201 gctgtcgcga catcctgcac ggacgcggga cgcaggcatg tcttgccggg gatgaccatg
     1261 ttgggcttga gcagcatcgt ctccagcga
//
LOCUS       DBNGZC010000893         3583 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2030992, whole genome shotgun sequence.
ACCESSION   DBNGZC010000893 DBNGZC010000000
VERSION     DBNGZC010000893.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2030992"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1532)
                     /gene="glpK"
                     /locus_tag="AC16G0_15485"
     CDS             complement(12..1532)
                     /gene="glpK"
                     /locus_tag="AC16G0_15485"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIR5101056.1"
                     /translation="MERYILALDQGTTSSRAILFDRNATIVAEAQREFRQIFPQPGWV
                     EHDPLEIWESQISVAREAVASAGIAPSQIAAIGITNQRETSIVWDRATGAPVHNAIVW
                     QCRRSAGLCDGLKARGLESAVRARTGLVIDAYFSGTKIQWLLENVPGLRARAERGAVC
                     FGTVDTWLTWRLSGGRAWVTDYSNASRTMLFNIHRLDWDPELCAALGIPRTMLPEVRP
                     SSAVYAHTLPNLLGAEIPIAGIAGDQQASLFGQACFHPGMAKNTYGTGCFLLVHTGDR
                     PVPSQSGLLTTIAASPAEGRAEYALEGSIFITGAAIQWLRDGLQVIATSAESEEVAAS
                     VPDTGGVYFVPAFVGLGAPHWDMYARGTVLGITRGTTRAHLVRAALEAIAYQTREVLG
                     AASADAGLRLQELRVDGGSTANNLLMQFQADILGTPVVRPVVKETTALGAAYLAGLAV
                     GYWTSREEIAQNWAVDRRFLPGMDQAKREDLFATWRRAVARAAGWALPPGGPANMR"
     gene            complement(1599..2450)
                     /gene="ugpE"
                     /locus_tag="AC16G0_15490"
     CDS             complement(1599..2450)
                     /gene="ugpE"
                     /locus_tag="AC16G0_15490"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013499904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HIR5101057.1"
                     /translation="MATPRRRESWIPHFVLLLGVVVFAFPIYFALIGSTHDAAIIATG
                     RMPLLPGGHMLENYWQALTSGTGFRVSASPVRTLMINSLIMALIIAVGKIAISIISAY
                     AVTFFSFPLRMFFFWMIFVTLMLPVEVRIIPTYKVLTDLGMVNTFSGLTIPLIASATA
                     TLLFRQVFLTIPDELVEAAKIDGAGPLRFFWQMVLPLSRTNIAALFVILFIYGWNQYL
                     WPLLITTSRSMETVVIGIVKMIGTSEAQTDWNLIMATAVLAMLPPVAVVIFMQRWFVK
                     GLIETEK"
     gene            complement(2450..3328)
                     /gene="ugpA"
                     /locus_tag="AC16G0_15495"
     CDS             complement(2450..3328)
                     /gene="ugpA"
                     /locus_tag="AC16G0_15495"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HIR5101058.1"
                     /translation="MRRRVIFPNKILPYVLVAPQLAITLAFFFWPAAQAIYYSVLLQD
                     PFGLRTQFVWFENFRTILTDPLYLGSIQVTVVFSVAVAALAMGVALLLAVMADTGIRG
                     TRIYRTLLMWPYAVAPAVAASLWLFIFHPSIGILGRALNGMGILWDYKLNGTQALLLV
                     ILASAWKQVSYNFLFFLAGLQAIPKSVIEAASIDAGPARRFRSIVFPLLSPTTFFLLV
                     VNLVYAFFDTFGVIHALTGGGPGKATETLIYKVYTDGVMNLDLGGSSAQSVILMAFVI
                     TLTALQFRFIERKVHY"
BASE COUNT          682 a         1154 c         1193 g          554 t
ORIGIN      
        1 aaaacgccaa actacctcat gttcgctggc ccgccggggg ggagcgccca cccggccgcg
       61 cgcgccaccg cccgccgcca ggtggcgaag aggtcctcac gtttcgcctg gtccatcccg
      121 gggaggaatc gtcggtctac agcccagttc tgggcgatct cctcccggct cgtccaatag
      181 cccacggcca gaccggccag gtacgccgct cccagcgccg tcgtctcctt caccaccggc
      241 cggaccacgg gcgtccccag gatgtcggct tgaaactgca tcaggaggtt gttggccgtg
      301 gacccaccgt ccacccgcaa ctcctggagg cgcagccctg cgtctgccga ggccgccccc
      361 aggacctcgc gggtctgata ggcgatggcc tccagcgccg ctcgcacaag gtgcgcccgc
      421 gtggttcctc gggtgattcc caggacggtg ccacgggcat acatatccca gtgcggggcc
      481 cccagtccca cgaaggccgg gacgaaatag acgccgccgg tgtccggaac ggaggcggcc
      541 acttcttccg actccgccga ggtggcgatc acctggaggc cgtctcgaag ccactggatc
      601 gccgccccgg tgatgaagat gctcccttcc agggcgtact ccgcccgacc ctccgcgggg
      661 ctggcggcaa tggtcgtgag cagaccggat tgcgagggca cgggacgatc tccggtatgg
      721 accaggagga agcagccggt tccgtaggta ttcttggcca taccagggtg aaagcatgcc
      781 tggccgaaaa gcgaggcctg ctgatccccg gcgatgccgg cgatggggat ctctgcgccc
      841 aggaggttcg gtaaggtgtg ggcgtaaacg gcgctggacg gtcggacctc cggtagcatc
      901 gtgcggggga tgcccagcgc cgcgcacagc tcgggatccc agtccagcct gtggatgttg
      961 aagagcatgg tgcgagaagc gttgctgtaa tcggtcaccc aggcccgccc gccggagagc
     1021 cgccaggtga gccaggtgtc cacggttccg aagcagaccg ccccccgttc ggcccgggcc
     1081 cggaggcccg gcacattctc cagcagccac tggatcttcg tcccggagaa gtaggcgtcg
     1141 atcaccagac cggtccgcgc gcgcaccgcg gactccagcc cccgggcctt gaggccatcg
     1201 cacagccccg ccgaacggcg gcactgccac acgatggcgt tgtgcacggg cgccccggtg
     1261 gcacgatccc acacgatgct cgtctcacgc tggttcgtga tcccgatggc cgcgatctgg
     1321 gatggcgcga tccccgccga agcgaccgcc tcgcgggcca cgctgatctg gctctcccag
     1381 atctccaggg gatcgtgctc cacccagccg ggttgaggaa agatctgccg gaattcccgc
     1441 tgcgcctccg ccacgatcgt tgcgttccga tcgaagagga tggcccgaga actcgtcgtg
     1501 ccctggtcca gcgccaggat gtagcgctcc atgaaacccc cgggcaattt ggcgactggg
     1561 cagccgcgag gaacggtccc acccgagtgc cagtttgcct acttttccgt ctcgatgagg
     1621 cccttcacga accagcgctg catgaagatc accaccgcca ccgggggcag catggccagg
     1681 acggccgtcg ccatgatgag gttccagtcg gtctgggcct cgctcgtccc gatcatcttc
     1741 acgatgccga tgacgacggt ctccatgctt cggctggtcg tgatcaggag cggccacagg
     1801 tactggttcc agccgtagat gaacaggatc acgaacaggg cggcgatgtt ggtgcgcgac
     1861 agcggcagga ccatctgcca gaagaagcgc agcggcccgg ccccgtcgat cttggccgcc
     1921 tcgaccagct cgtccgggat ggtgaggaac acctggcgaa agagaagcgt cgccgtggcc
     1981 gaggcgatca ggggaatggt gagcccggag aacgtgttga ccatcccgag atccgtcaag
     2041 accttgtagg tcgggatgat gcgcacctcc acgggcagca tgagggtcac gaagatcatc
     2101 cagaagaaga acatgcgcag cgggaagctg aagaaggtca cggcataggc ggagatgatg
     2161 gagatggcga tcttccccac ggcgatgatc agggccatga tcaggctgtt gatcatcagg
     2221 gtccggacgg gagacgccga gacgcggaaa cccgtgccgc tggtcagcgc ctgccagtag
     2281 ttctccagca tatgcccccc cggcaggagc ggcatccggc ccgtggcgat gatggcggcg
     2341 tcgtgggtgg aaccgatcag ggcgaagtag atgggaaatg cgaacaccac cacgcccagg
     2401 aggagcacga agtgcgggat ccagctctcg cggcgcctgg gtgtggccat cagtagtgga
     2461 cctttcgctc gatgaagcgg aactggagcg cggtgagcgt gatcacgaac gccatcagga
     2521 tcaccgactg cgccgaggag ccgcccaggt ccaggttcat gacgccatcg gtgtacacct
     2581 tgtagatcag ggtctcggtg gccttgcccg gcccgccgcc tgtcagcgcg tggatcacgc
     2641 cgaaggtgtc gaagaaggcg tagaccaggt tgaccacgag caggaagaag gtggtcggcg
     2701 acaggagcgg gaagacaatg ctccggaaac gacgcgccgg tcccgcgtcg atggaggccg
     2761 cctcgatcac cgacttcgga atcgcctgca gacccgccag gaagaacaga aagttgtagc
     2821 tgacctgctt ccaggccgag gccaggatga ccaggaggag cgcctgcgtc ccattcagct
     2881 tgtaatccca gagaatcccc atgccgttca gggcgcggcc caagatcccg atcgaggggt
     2941 gaaaaatgaa gagccagagg ctcgcggcga ccgcgggcgc cacggcgtac ggccacatga
     3001 ggagcgtccg gtatatccgc gtgcctcgaa tgcccgtgtc cgccatcacc gccaagagca
     3061 gcgccacccc catggccagc gccgccacgg cgaccgagaa gacgaccgtg acctggatgg
     3121 agcccaggta cagggggtcg gtgaggatgg tgcggaagtt ctcgaaccag acgaactgcg
     3181 tgcggaggcc gaaggggtct tggagcagga ccgaatagta gatcgcctgg gcggccggcc
     3241 aaaaaaagaa ggccagcgtg atggcgagct gtggtgcaac caggacgtat gggagaatct
     3301 tgtttggaaa gatgactcgc cggcgcatca gcgactccgg gtcgcggtca gactttgttc
     3361 tcgcatgccg tcatgccggc gaaagccggt atctagacag gccgctcttc ctcgaataac
     3421 ccattcccat gctggacccc ggcattcacc ggggtgacga acgtcaagtg ccccgtaaca
     3481 cagttcactg gttcgcgcgg acttcgcacg aaccccgctc cctctcccac accctctccc
     3541 cgggtgcggg gagaggattg gggtgagggg gtgctactgg cgg
//
LOCUS       DBNGZC010000894         2314 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_373411, whole genome shotgun sequence.
ACCESSION   DBNGZC010000894 DBNGZC010000000
VERSION     DBNGZC010000894.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_373411"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="AC16G0_15500"
     CDS             complement(<1..1022)
                     /locus_tag="AC16G0_15500"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5101059.1"
                     /translation="MTSDRQFRTPLFDALVALAEAKKVSFHTPGHKSGKGISTRFRKF
                     VGSKIFTIDLTTLDEVDCLQKPVGVIKEAQELAAEAHGADRSFFLVNGTTVGNHAMVL
                     STVLPGDEVLVARNAHKSILAGIILSGATPRFFLPDFDADLGIAVNVSAAAAEAAMRA
                     HPQARVVALTSPNYYGIAADIPTIVAAGQRQCRLLIVDEAHGPHLHFHPALPASAVDA
                     GADLTVQSTHKIVGGMTQASMLHLKGGAVDAVRVSGVLKMLQSTSPSYILMASLDLAR
                     MQMSTEGRKLLGKAIELAEEARGRINRIAGLHCLTALQVKEWGDSSLDVTKLTISVKE
                     IGLTGYA"
     gene            complement(1119..2036)
                     /gene="speE"
                     /locus_tag="AC16G0_15505"
     CDS             complement(1119..2036)
                     /gene="speE"
                     /locus_tag="AC16G0_15505"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HIR5101060.1"
                     /translation="MPLKNYRWFIEYFSSEEGHLHGIRRVLFSKETPFQSMDILELGS
                     YGKSLVLDGRIQSTSRDEFIYHETLVHAAMLTHPEPRRVFIVGGGEGATLREVLRHKT
                     VERALMVDIDEEVVARSKELLPEWHQGAFDDRRTEVRYLDARKYLEETSETFDVVIID
                     ISEPIEEGPAYLLFTKEFYEIVQERLSPAGVISLQAGTISLNDLECYAAIQKTLEKVF
                     PVVAPCWASVPSFALPWGFSVASKQQDPRLLTPEDINKLVADRIIGDLRYYDGITHQA
                     SFLLPKHLRARLASELRIIEDNHPLFAWS"
     gene            complement(2005..>2314)
                     /gene="speD"
                     /locus_tag="AC16G0_15510"
     CDS             complement(2005..>2314)
                     /gene="speD"
                     /locus_tag="AC16G0_15510"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HIR5101061.1"
                     /translation="VDVAFHEFNPFGVSGVVVIAESHLSIHTWPEYRYAAVDIFTCGD
                     VIKPERAAAYVASRFRCKNPSIIEMKRGLIPGVVGKLAHKITATEGVADAAQELSLVH
                     "
BASE COUNT          408 a          724 c          763 g          419 t
ORIGIN      
        1 gcataccccg tcaggccgat ctctttcacc gagatggtga gcttggtcac atccaggctc
       61 gaatcccccc actccttgac ctgaagggcc gtgaggcaat ggagacccgc aatcctgttg
      121 atccgccccc gagcctcctc ggccagctcg atggccttcc ccaggagctt ccgcccctcc
      181 gtggacattt gcattcgggc caagtccagg gacgccatga ggatatacga cgggctggtg
      241 ctctggagca tcttgaggac gcccgacacg cgcacggcat ccaccgcccc tcccttgagg
      301 tgcagcatgg aggcctgggt catgcccccg acgatcttgt gggtgctctg caccgtgagg
      361 tcggcccccg cgtccaccgc cgaggcgggg agggctggat ggaagtgcag atgcggccca
      421 tgcgcctcat ccacgatgag cagcctgcac tggcgctgtc cggcagcgac gatggtgggg
      481 atgtcggcgg caatcccata gtagttgggg ctggtgaggg ccaccacccg cgcctgcgga
      541 tgggcgcgca tggcggcctc ggcggcggcc gcactgacgt tcacggcaat gcccaggtcc
      601 gcgtcgaagt ccggcaggaa gaagcggggt gtcgcaccgc tcaggatgat acctgccagg
      661 atggacttgt gggcgttccg tgccaccagg acctcgtccc cgggcagaac cgtggacagg
      721 accatggcgt gattccccac cgtcgtcccg ttcaccagga agaaggagcg atcggccccg
      781 tgagcctccg ccgccagctc ttgggcctcc ttgatcactc ccaccggctt ttgcagacag
      841 tccacctcgt ccagggtggt gagatcgatg gtgaagattt tgcttccgac gaatttgcga
      901 aaccgggtgg agatgccctt cccgctcttg tgaccggggg tatggaagga gaccttcttg
      961 gcctcggcca aggcgaccag ggcgtcaaag agcggcgtgc ggaattgccg gtcggacgtc
     1021 atgacgggat acccgcctgg cggtcagcgc atcggagcgc caacgggaag cgcaggacca
     1081 gttcggggtc gcctgcagcc ccaggcccca acgggcgctc agctccatgc aaagagggga
     1141 tggttgtcct cgatgattcg cagctcgctg gccaggcggg cccggaggtg ctttggtagg
     1201 aggaagctcg cctggtgcgt gatgccgtcg tagtagcgga ggtcaccgat gatccgatct
     1261 gccaccagct tgtttatgtc ctccggcgtc agaagacggg ggtcctgctg cttggacgcc
     1321 acggagaatc cccagggcag ggcaaaggac ggcaccgagg cccagcaagg agccacgacc
     1381 ggaaacacct tctccagggt cttctggatg gccgcatagc attccagatc gttcaggctg
     1441 atggtgccgg cctggagaga gatcacgccg gcaggtgaga gccgctcctg cacgatctcg
     1501 tagaactcct tcgtgaagag gaggtaggcc gggccctcct cgatcggctc ggagatgtcg
     1561 atgatgacca cgtcgaaggt ttccgaggtc tcttccaggt atttgcgggc gtccaggtac
     1621 cggacctccg tgcggcggtc gtcgaaagct ccctggtgcc actcggggag caattccttg
     1681 ctgcgggcca cgacctcttc atcgatgtcc accatgagcg ctcgctcgac cgtcttgtga
     1741 cggagcacct cgcggagcgt ggccccctcc ccgcccccca cgatgaacac ccgccgcggc
     1801 tcgggatggg tgagcatggc ggcatggact agcgtctcgt ggtagatgaa ctcgtctctg
     1861 ctcgtggact ggatccggcc gtcgaggacc agggacttgc cgtagctccc cagctcgagg
     1921 atgtccatgg attggaaggg ggtttccttg gagaacagga cgcgccggat gccgtggagg
     1981 tgtccctcct cggagctgaa atactcaatg aaccagcgat agttcttgag cggcatcggc
     2041 cacgccctca gttgcggtga tcttgtgagc cagcttcccc accacgccgg ggatcaggcc
     2101 gcgcttcatc tcgatgatcg aggggttctt gcagcggaag cgggaggcca cataggcggc
     2161 ggcgcgttcc ggtttgatca catcgccgca ggtgaagata tccaccgccg cgtaccggta
     2221 ctcgggccag gtatgaatcg agaggtgcga ctctgcgatg accacgacgc cgctcacccc
     2281 gaagggattg aattcgtgaa aggcgacgtc cacg
//
LOCUS       DBNGZC010000895         2968 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_564324, whole genome shotgun sequence.
ACCESSION   DBNGZC010000895 DBNGZC010000000
VERSION     DBNGZC010000895.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_564324"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="AC16G0_15515"
     CDS             <1..452
                     /locus_tag="AC16G0_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017321080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome C peroxidase"
                     /protein_id="HIR5101062.1"
                     /translation="PDELVNVDGALKVPSLRNVELTAPYFHNGGQATLEQVVAYYNRG
                     GDFALENKDNIDPNVHPLLLTPVEREALVAFLKALTDDRVRYEKAPFDRPSLNVPNGG
                     SGHITEMFGASVMDDRIEIPAVGAGGNGLGLGTPVATGTPLRNFLQP"
     gene            708..>2968
                     /locus_tag="AC16G0_15520"
     CDS             708..>2968
                     /locus_tag="AC16G0_15520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HIR5101063.1"
                     /translation="MYLPWDASKARVKEAENARKNRMEIVQALSHGQVSRRDLIKWGL
                     FTTAGLLVAKQGLSPFAKSVYADGGGGGGSIPTGAPRSPLFGAKAFTQPMARFDVLPW
                     KSPLLLSPAPTAEANQTLQPVPAVLGGGYGPVEGRPPGPIWAHQRWSEYSCKVAVEAT
                     QEGAKVNTVYNPGVPSRLNSGIDPSAAIPLRFHPQMPTQSPLSVWTFNGTIPPKLVQG
                     RYGEPMLFRHHNKLPYDVTQNGGFGRHTISTHEHNGHHGAENDGFTGAYFFPGQFYDY
                     HWPIVHAGCSSMNLEATDPRCGSPDDNGGIVKLPGDWHETMSTHWFHDHMFSFTSQNV
                     YKGNAAMFNIYSSLDRGNEAINDGVNLRLPSGTVNAWGNLDYDVNLMLADKAWDGQGQ
                     LYFDIFNFDGFLGDVMTVNLAYKPYFEVERRKYRFRILNASVSRFFKLALSDGSPMIQ
                     IANDGNIFPHPVVLTELDEQGIAERYDIVIDFSRYTVGDKVWMVNLCEHRDGRGPARD
                     VSLAEALSGQSPDPCVGKFLEFRIVRDPASPDLSQVPTTMIPNPDLSQIPVARERVFE
                     FGDGGNQSSSNPITAFVGPWGIKTDGGAMLNADYSRVSAAPKYGTREVWTLKNGGGGW
                     DHPIHIHFEEGQILARNGSAGLVPAWERGRKDVYRLRPGGSVTITLQFRDWGGMFMEH
                     CHNTVHEDNAMLLRWEIDDSGAPMLRPLPTPIPTPQGVTFIDPNDVLSTNVSATTGSE
                     NITSGGNGTNVRI"
BASE COUNT          563 a          989 c          926 g          490 t
ORIGIN      
        1 accccgacga gctggtgaac gtggacgggg cgttgaaggt gccctcgctc cggaacgttg
       61 agctgaccgc tccctatttc cacaacgggg gccaggcgac gctggagcag gtggtggcgt
      121 actacaatcg gggaggcgac tttgcgctgg agaacaagga caacatcgac cccaacgttc
      181 acccgctcct gctcaccccc gtggagcggg aagccctggt cgccttcctg aaggcgttga
      241 ccgacgaccg ggtgcgctac gagaaggccc ccttcgatcg tcccagcctc aacgttccta
      301 acggcggaag cggccacatc accgagatgt tcggtgcttc ggtgatggac gaccgtatcg
      361 aaatcccggc tgtcggggct gggggcaatg gccttggtct tggaacgccc gtggccacgg
      421 gaacgccgct gaggaacttc ttgcagccgt agtggccgtc gatgtgaacc tgtcgcagcg
      481 tctgctcgtc agggcgctgc ggcgagatgg gatgtcatga tgatcgtgat ggtagacact
      541 tcggatagaa ccggcaacgc agcgctggca agcactctga ccgccggttt caaataaagc
      601 atgttgaatc tcggcggggg tgaatacgcc ttctgccaag gctgggttgg gcctacagct
      661 tctcatcaac gacactgcgg tacgcggtcg cactaggagg aacgccaatg tatcttccat
      721 gggacgcctc gaaggcgagg gtgaaggaag cggaaaacgc ccggaagaac cggatggaga
      781 tcgtgcaagc cctctcccac ggccaggtga gccgccgaga cctcatcaag tggggactct
      841 tcaccaccgc gggcctcctg gtcgcgaagc agggcctgag ccccttcgcc aagagcgtct
      901 acgctgacgg tggtggcggt ggtgggagca tccccacggg tgccccgcgg agtccactgt
      961 tcggcgccaa ggccttcacc cagcccatgg cccggttcga cgtcttgccg tggaagtcgc
     1021 ccttgctcct gagcccggcg cccacggcgg aggcgaacca gaccctccag cccgtgcccg
     1081 cggtcctggg cgggggctac ggccccgtcg agggacgccc cccgggcccc atctgggccc
     1141 accaacgctg gagcgaatac tcctgcaagg tggcggtgga ggccacgcaa gaaggggcca
     1201 aggtcaatac cgtctacaat ccgggcgtgc cctcgcgcct caattccggc atcgacccga
     1261 gtgcggccat ccccctgagg ttccaccccc agatgccgac ccagtctccc ctgtcggtct
     1321 ggaccttcaa cgggaccatc ccccccaagc tggtgcaggg gcggtatggc gagcctatgc
     1381 ttttccgcca ccacaacaag ctcccctatg atgtcaccca gaacgggggc ttcgggcggc
     1441 acaccatctc cacgcacgaa cacaacggcc accacggggc ggagaacgac ggcttcactg
     1501 gggcctactt cttccccggg cagttctacg actaccactg gcccatcgtg catgccggct
     1561 gctccagcat gaatctggaa gccacggatc cccgctgcgg cagcccggat gacaatgggg
     1621 ggatcgtgaa gctccccggc gactggcacg agaccatgag cacccactgg ttccacgacc
     1681 acatgttcag cttcacctcc cagaacgtct acaagggcaa cgccgccatg ttcaacatct
     1741 acagctccct tgatcggggg aacgaagcca tcaacgacgg cgtgaacctg cgcttgccca
     1801 gcgggacggt gaatgcctgg ggaaatctcg attacgacgt gaatctgatg ctggcggaca
     1861 aggcctggga cggccagggg cagctctact tcgacatctt caacttcgac gggttcctgg
     1921 gggatgtcat gaccgtcaac ctggcctaca agccctactt cgaggtggag cgccggaagt
     1981 accgattccg catcctgaac gcctctgtgt cccgcttctt caagctagcc ctgagcgacg
     2041 gctcccccat gatccagatc gccaacgacg ggaacatctt cccccacccg gtagtcctca
     2101 cggagctgga cgaacagggg atcgccgagc gctacgacat cgtcatcgac ttctcccgct
     2161 acaccgtggg ggacaaggtc tggatggtga acctctgcga gcaccgggac ggaagggggc
     2221 ccgccaggga cgtctcgttg gcagaggctc tctccggcca atcccccgat ccctgcgtgg
     2281 gcaagttcct ggagttccgg atcgtgcggg acccggcctc ccccgatctc agccaggtgc
     2341 cgacgaccat gattccgaac ccggatctct cccagatccc cgtggcccgg gagcgggtct
     2401 tcgagttcgg ggatggcggg aatcagtcca gctccaatcc catcacggcc ttcgtcggcc
     2461 cctggggtat caagaccgat ggcggggcca tgctgaacgc cgactacagc cgggtctcgg
     2521 cggcccccaa gtacggcacc cgcgaggtct ggaccctgaa gaacgggggc ggcggctggg
     2581 atcaccccat ccacatccac ttcgaggagg ggcagattct cgcccggaac ggcagcgccg
     2641 ggctggtgcc tgcgtgggag cgcggtcgga aggatgtcta ccgcctccgc cccggcggga
     2701 gcgtcaccat caccctgcag ttccgggact ggggcggcat gttcatggag cactgccaca
     2761 acacggtgca cgaagacaac gccatgctgc tccgctggga gatcgatgac agcggggccc
     2821 ccatgctgcg gccgctcccg accccgatcc cgacacctca gggggtgacc ttcattgacc
     2881 cgaatgacgt cctgtcgacc aacgtcagtg cgacgaccgg cagcgagaat atcaccagtg
     2941 gcgggaatgg gaccaacgtc cgtatcag
//
LOCUS       DBNGZC010000896         3929 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1839196, whole genome shotgun sequence.
ACCESSION   DBNGZC010000896 DBNGZC010000000
VERSION     DBNGZC010000896.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1839196"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="AC16G0_15525"
     CDS             complement(<1..503)
                     /locus_tag="AC16G0_15525"
                     /inference="COORDINATES: protein motif:HMM:NF014375.7"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HIR5101064.1"
                     /translation="MRVLLVSVNRESVPYPVAPLGVSYIAGAARSAGHEVEVLDLCFS
                     PDIQGDIDRAVRRFVPELIGVSIRNVDNLTFPSSVSYLPEIHTAVAALKRVSRAPMVA
                     GGPGFSIFPEQLLSLLDLEFGIIGEGEETFCLLAKYLGNGGGVPGLPSLIRRGGAVPK
                     ETQGCCS"
     gene            complement(508..1584)
                     /locus_tag="AC16G0_15530"
     CDS             complement(508..1584)
                     /locus_tag="AC16G0_15530"
                     /EC_number="1.17.98.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5101065.1"
                     /translation="MSHELEQELGFSQEEIAGCLDKQGLLSLELEFTKRCNLRCTYCY
                     SSAGDALGQELTLRELLGVIEQARELGARKIILLGGGEPFLFTQVKEVIRHIHSLGLS
                     QAVFTNGTLLTRELCRFLYDHRVSVAIKGNSFRPAIQDSLAGVTGAFGSIARGLRLLM
                     EEGYPAKDCSLCLQSVVCRPNMEEIQEMWVWARDRGITPYFEVLTNQGRARQNPDLAL
                     TPGETHTIFQTLSEIDSRGYDFHWLPRPPIAGFTCRRHLYSCLVNSQGFVQPCTGVDV
                     SIGNIREKPLGALLRESRVIHDLRGIYRRIDPKCQTCGFAGECYGCRGNAYQATGSYL
                     AADPACWLIQGNGAGARCGLGRER"
     gene            complement(1581..2894)
                     /locus_tag="AC16G0_15535"
     CDS             complement(1581..2894)
                     /locus_tag="AC16G0_15535"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HIR5101066.1"
                     /translation="MRFLPDIYALAPSEITQIQEGLLQETLVRAARTSPFYRERFSGG
                     TVNAAGVRTLADLSRLPLTSKEDIQERNQDFWAAPRDQLKELVATTGTTGRPVYIAMT
                     DPDLERLGENERRGFSWLGAKPGERFHIAVTLDNLFIAGLAYQWGLRMLGVATIRVGA
                     QPPRRHLDLMKQLRPEGVVAVPSLLLALARQAEKDGDELGTFAPRRAMLIGEAIRGQS
                     LASNTLGRLLEDSWGGVLSSTYGLTEAGLAYHECPMHQGLHSHPDLVVSEIVDDAGCS
                     LPEGQAGELVITTLQVEGMPLIRYRTGDITFIIPGACPCGRGGPRIGPILGRKQHRLK
                     VKGTTLYPRTIEDALLSMEGVENFVIEAHRGDDETDRLIVRIGTRRDDLGFQEMVKDT
                     LYAKARVTPEVHLASPAEVEGLLFEGGRRKPRVFLDLRKAQGGVR"
     gene            complement(2891..3862)
                     /locus_tag="AC16G0_15540"
     CDS             complement(2891..3862)
                     /locus_tag="AC16G0_15540"
                     /inference="COORDINATES: protein motif:HMM:NF012337.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HIR5101067.1"
                     /translation="MSPPVRVSGIGMARSSDGGAKAEDTPDQRLAVVLSDLSRGGLPV
                     RLFHAIEEVACQAAYEALSRAAVAVPFGGEGIGIAIGVDEGIDGIKARYYQGILTDGP
                     LGASPLAFPFTTPNTIAARISILFGLRGESHTLCGGSLSGAQAIGLAIEALRAGRSGA
                     VLAGVATSVEREFLDALCRLGKPDGGQPRCGACFLLLEPQAFTAETKWVAELIGYGEG
                     FGKDETTDAVQTCLDDAGLSPEHVQSVRVASVNDARSLAASLHRVGVKAPMVRSPGSD
                     LYSASFPMAVAEAAQQAANRTPEPVLVVGWDCVTGASAALVRSQGRP"
BASE COUNT          729 a         1328 c         1201 g          671 t
ORIGIN      
        1 aaggagcaac acccttgagt ctctttgggg acagctcctc cgcgtcgaat gaggctcggg
       61 aggccaggga cccctccccc gttgccgaga tacttggcca gaaggcagaa ggtctcctcc
      121 ccttccccga tgatcccgaa ctccaggtcg aggagggaca ggagctgctc cgggaagatc
      181 gagaaccccg gccctccagc caccatgggc gcccgagaga cccgtttcag tgccgccaca
      241 gccgtgtgga tctctggaag gtaggacacc gaggacggaa aggtcagatt gtcgacgttt
      301 ctgatggaga cgccgatcag ctccgggacg aatcggcgaa cggctcggtc gatatctcct
      361 tggatgtccg gcgaaaaaca gagatcgagc acctccactt catggccggc gctgcgggcc
      421 gctccggcga tgtaggatac cccgagcggt gccaccgggt aggggaccga ctctcgattc
      481 accgagacta gaagtacccg catgcctcta tctctcgcgc ccaagcccgc agcgggctcc
      541 cgcaccattc ccctgaatga gccagcaggc tgggtcggcc gcgagatagc tccccgtggc
      601 ctgataggcg tttccccggc aaccgtaaca ctctcctgcg aatccacacg tctggcactt
      661 gggatcaatc cgccggtaga ttccgcggag gtcgtgaatc accctgcttt cccgaaggag
      721 cgctcccagg ggcttctctc ggatgtttcc aatcgataca tcgacgccgg tgcagggctg
      781 gacaaacccc tgagaattga ccaggcaact atacaggtga cgccggcagg tgaaccccgc
      841 gatgggcggc cttggaagcc aatggaaatc gtatcccctg gagtcgatct ccgaaagcgt
      901 ctggaagatc gtgtgggtct ctccgggtgt cagggcgaga tcggggttct gtctggctcg
      961 tccttgattg gtcagcacct cgaaatacgg agtgatgccc cgatcccgag cccagaccca
     1021 catctcttgg atctcctcca tgttcggccg gcagaccacg gattggaggc agagagaaca
     1081 atccttcgca gggtaaccct cctccatcag gaggcgcaac ccccgggcga tcgacccaaa
     1141 ggcccccgtc acccccgcca ggctgtcctg gatggcgggg cgaaagctgt tgcctttgat
     1201 ggccacgctc acgcgatggt catacaggaa acgacacagc tcccgggtga ggagcgtccc
     1261 gttggtaaag accgcctggg agaggccgag ggagtggatg tgccggatga cctccttgac
     1321 ctgagtgaag aggaatggct cccctccccc gaggaggatg atcttcctgg cccccaattc
     1381 tcgcgcctgc tcgatcactc ccaggagctc acggagcgtc aattcttggc ccagggcgtc
     1441 gcctgcagag gagtagcaat aggtgcagcg gaggttgcac ctcttggtga actccagctc
     1501 caaggagagc agcccctgct tgtccagaca gcccgcaatc tcttcctggg aaaatcccag
     1561 ctcctgctcc aattcgtgac tcaccgcacg ccaccctgag cctttcgcag atccagaaac
     1621 accctgggtt ttctccgccc tccctcgaac aacagacctt cgacttctgc cggagacgcc
     1681 aggtgaacct ccggggtgac ccgggctttg gcgtacaggg tatccttgac catctcttgg
     1741 aagccaagat cgtcccgccg ggtgccgatg cggacgatga gtcggtccgt ctcatcgtca
     1801 ccccgatgcg cctcgatcac aaaattctcc acgccctcca tcgagagaag ggcatcctcg
     1861 atggttctcg gatacagggt ggtccccttg accttcagcc ggtgctgctt gcggcccagg
     1921 atcgggccga tgcgggggcc ccctctgccg cacgggcagg cgccagggat gatgaaggtg
     1981 atatcccccg tccggtaccg gatgagcggc atgccttcca cctgcaaggt cgtgatgacc
     2041 agctctcccg cctgtccctc tggaagcgag cacccggcgt cgtcgacgat ctcgctgaca
     2101 accaggtccg ggtgcgagtg gaggccctgg tgcatcgggc actcgtgata cgccagacct
     2161 gcttcggtga ggccgtaggt cgacgacagc actccccccc aggaatcttc gagaagacgg
     2221 ccgagggtgt tcgaggcgag gctctggccc cggatggcct cgccaatcag catcgcccgt
     2281 cgtggggcaa aggttccaag ctcgtcacca tctttctctg cctgacgtgc cagggcgagg
     2341 agcagggagg gaacggcgac caccccctca ggtcgcaact gcttcatcag gtcgaggtga
     2401 cgccggggcg gctgggcccc aacccgaatc gtggcaacgc ccaacatgcg cagtccccat
     2461 tggtatgcca gccccgcgat gaagaggttg tccagggtga ctgcaatgtg gaatcgctcg
     2521 ccgggcttcg ctcccaacca ggaaaacccg cgtcgctcgt tctcccccag gcgttccagg
     2581 tccgggtccg tcatcgcgat gtatacaggt ctccccgtcg tccccgtggt ggcaacaagc
     2641 tccttgagtt ggtccctcgg agcagcccaa aaatcctgat tgcgctcctg gatgtcctcc
     2701 ttggacgtga gcggaagacg cgagagatcg gccaacgtcc ggaccccggc cgcattcacc
     2761 gtcccgccgg agaaccgttc ccgatagaag ggagaggtgc gcgcagcccg gacgagcgtt
     2821 tcctggagca gtccttcctg gatttgcgtg atctcggaag gcgccagcgc atagatgtcc
     2881 ggcaagaatc tcatggtcgt ccctggctcc ggacgagcgc agccgatgca cctgtcacac
     2941 aatcccatcc aacgacgagg accggctccg gcgtcctgtt cgctgcctgc tgtgccgcct
     3001 ccgccacggc catggggaag gaggcggaat acaggtcgga gccgggagat cggaccatgg
     3061 gcgccttcac gcccacccga tgaagcgacg ccgccaggga acgggcgtcg ttcaccgacg
     3121 ccacccgcac cgattggacg tgttccgggg agagcccggc gtcatccaag caggtctgaa
     3181 ccgcgtccgt ggtctcatcc ttcccgaacc cttccccata cccgatcaac tccgctaccc
     3241 acttggtctc agcggtgaag gcctgcggct ccagaaggag aaagcaggct ccgcatctgg
     3301 gctggccgcc atcgggtttg ccaaggcggc acagagcgtc gaggaattcc cgctccaccg
     3361 aggtcgccac gcccgccagc acggccccgg accgtccggc ccggagggct tcgatcgcca
     3421 ggccaatcgc ctgggccccc gaaaggcttc ctccgcacag cgtatgactc tcgccccgga
     3481 ggccgaacag gatcgagatt cgcgcggcga tggtgttggg ggtcgtaaac ggaaaggcca
     3541 gcggactcgc gcccagcggt ccgtcggtca ggatcccctg gtagtagcgc gccttgatcc
     3601 catcgatccc ctcgtccacc ccgatggcga ttccgatgcc ctcgcccccg aaaggtacgg
     3661 caaccgcggc gcgggagagg gcctcgtacg ccgcctggca cgccacctcc tcgatggcat
     3721 ggaacaggcg caccggcagc cctccccgcg agaggtctga gagcaccacc gcgagacgct
     3781 ggtcgggtgt gtcctcggct ttcgcgccgc cgtccgacga ccgcgccatc ccgattccgg
     3841 agacccggac gggcgggctc atgacccacc cccccggatc agcaaggcgg cgttttgccc
     3901 tccgaacccc gcggagaggg agagggcgg
//
LOCUS       DBNGZC010000897         4103 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1252964, whole genome shotgun sequence.
ACCESSION   DBNGZC010000897 DBNGZC010000000
VERSION     DBNGZC010000897.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1252964"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..447)
                     /locus_tag="AC16G0_15545"
     CDS             complement(256..447)
                     /locus_tag="AC16G0_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101068.1"
                     /translation="MAEFFVYVCSQCDEAYAGALEPNERRIDRKETTGICPECLETLF
                     GLNEGAESRRNYSGEAPKA"
     gene            966..3296
                     /locus_tag="AC16G0_15550"
     CDS             966..3296
                     /locus_tag="AC16G0_15550"
                     /inference="COORDINATES: protein motif:HMM:NF012364.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HIR5101069.1"
                     /translation="MRPLTGWIFDVYPTTGGMVVWILDTEGKSYRLVDPFQPSFYAAG
                     PVKALRNLAEELARLRLAAPVGMTRRQEFWTGEPRDILEFILLDPEKQPILLRRLATP
                     RPGIVLYDCDLAPSLTYCHARGIFPLAKVTVRPAGDTISNFEFRISNSEKTHRRMGPD
                     NPQWCYTVEGSAWDLGYPEPPLTLMALTSQGDEHGRGAPLGRLRSLTVACEGTTTAIE
                     EGDPETLLCELNCLIRRFDPHVIVTDWGDAFLVPTLLNLGRRANVSLEFDRDMVHRRI
                     RTAGRSYFSYGRVVYQAPSYPFFGRWHLDSKNSFILKETEMAGLLELARLSKVPVQKM
                     ARTSPGSAISMMQLDRALTDGILIPWKKGEPERWKTAWDLLVTDKGGLVYQPITGLYE
                     GVAEIDFASMYPAMMVHHNIWPETVDCACCPDNAAPEIGYSTCRRREGLVTKTLRPIL
                     ARRQELKRLRNAAAGPARARYDHRQTALKWILVTCFGYLGYKNARFGRIEAHETVTAY
                     AREKLLTARTLAEDRGFRVLHAIVDSMWIQRPGATETEVLNLCRAIQEAVGIGINLEG
                     IYKWVSFLPSRVDPAVSVANRYLGAFRSGELKVRGIDLRRSDTPPLVAEAQQRMLAIL
                     AQADDLAGFAARIPKVLEVFVEYAWRLREGQVRLEALIIKRTVSKEADEYKTNSQIGL
                     ASRQMADRGVAVHPGERVRYVISDAAAPNAEARVRAFPLVAADTSYDTGQYLGLLCKA
                     VETLLWHHGYDCRRLLDLLAERNVVADRSSAGRGRA"
     gene            complement(3415..3657)
                     /locus_tag="AC16G0_15555"
     CDS             complement(3415..3657)
                     /locus_tag="AC16G0_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101070.1"
                     /translation="MRRRAWVALLAIGVMVSLSGCGSWSRYLAEERQNGAHFATWQHM
                     GYSLFRATPKETTKMDVASAQQEKWWGDPILVEPIQ"
     gene            complement(3686..>4103)
                     /locus_tag="AC16G0_15560"
     CDS             complement(3686..>4103)
                     /locus_tag="AC16G0_15560"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0032508 - obsolete DNA duplex unwinding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HIR5101071.1"
                     /translation="NNYDKAVFNGDLGRLRSINAPDQAVVVQVDDRELTYDFSELDEL
                     TLAYAVTVHKSQGCEYPAVILPLHTTHYPMLQRNLLYTAITRARRLLVLVGTKKALAI
                     AVRNDVTLRRATRLADRLVGFERPTRPPLTAPEVPR"
BASE COUNT          738 a         1344 c         1245 g          776 t
ORIGIN      
        1 caccgaatag cgttctcgga aaccacgtgg gaccagatca acgagccagg tgcctacctc
       61 gagagaggta ctgcgatctc tatcgggtat ctcaggaggc gatttgacca gcgcctccga
      121 tgatcaagaa gcaaaacctc ggggcgtcac gcctcgttaa actttcaggt aacccgttcg
      181 tcaccgcgct ggaggccagg atggtgtgcg ccgaggccaa catccatcca aacttctgat
      241 cctctctggg gaccgtcagg ctttcggagc ttcaccggaa tagttcctcc ggctctccgc
      301 cccctcattc agcccgaaaa gtgtctcgag gcactcggga cagattccag tcgtctcctt
      361 ccggtcgatc cggcgctcat tcggttcgag cgcccctgcg tatgcctcgt cacactgcga
      421 gcacacataa acgaagaact ccgccattga atcctccctc ctgctgaccg gatccctgga
      481 cggcacatcg ccccgccctt aggttgaggg atggtcagtg caaggcgggg accattcggt
      541 tattggttat atccggcctc ctgcgagttg atgttacccc tctctacgga tccgcaagac
      601 cgccgtccct ttgcaacgac tgaacccaca cccatagatg ccttgtttcg catccgaaag
      661 aggagcgcgc atttccccgt cttcaatcat gaggcaatac ttgacggcga cgcgcgaagg
      721 cattgcaccc tgttcgatct caggactgac ttctcgctct ccggacctag atacgccacg
      781 aatggtgctg tgagctatgc cgcaaaggtg tgggaggttg gccagcaacc ggaacagaaa
      841 tatccaattg acagccatac atacgtttgg tattaacgga gataacgcag gaactttccg
      901 actatgcaag ctctggtaac gtccaatctt ccggtgtttt tctggagtcc tccatgttct
      961 ccgccatgcg acccctgacg ggctggatct ttgacgtcta tccgaccact gggggaatgg
     1021 tggtgtggat cctggacacc gaggggaaga gctaccgcct cgtggatcca ttccagccca
     1081 gcttctacgc ggcgggcccg gtcaaggccc ttcggaatct ggccgaggag ttggcccggc
     1141 tgcggctggc ggcgccggtg gggatgaccc gccgacagga gttttggacc ggtgagcccc
     1201 gcgatatcct ggagttcatc ctcctcgatc ccgagaagca gccgattctc cttcgtcggc
     1261 tcgcgacccc ccggcccggg atcgtcctgt acgattgcga cctcgcaccg tcgctcacgt
     1321 attgtcatgc gcgggggatc tttccacttg ccaaggtcac cgtccggccg gcgggggata
     1381 cgatttcgaa tttcgaattt cgaatttcga attcggagaa gacacatcgg aggatgggac
     1441 cggacaatcc gcaatggtgt tacactgtcg aggggtcggc gtgggatctg ggttatccgg
     1501 agccgccgct cactctgatg gccttgacca gccaagggga cgaacatggg cgcggcgctc
     1561 cgctggggag gctccgctcc cttaccgtcg cctgcgaagg gaccacaaca gccatcgagg
     1621 agggtgatcc cgagacgctc ctctgcgagc tgaactgcct gattcgccgc ttcgaccctc
     1681 acgtcatcgt cacggactgg ggcgacgcct ttctggtccc gacccttctc aacttggggc
     1741 gccgcgccaa cgtatcgctg gagttcgacc gcgacatggt ccaccgccgc atccggaccg
     1801 cggggcgttc gtatttttcc tacggccgcg tcgtctacca ggcgccctcc taccccttct
     1861 tcggccgctg gcatctggac tcgaagaact ccttcatcct gaaggagacg gagatggcgg
     1921 gcctcctgga gctggctcgc ctgtcaaagg tcccggtcca gaagatggcc cggacctccc
     1981 cgggctcggc catcagcatg atgcagctcg accgcgcgct caccgacggc atcctgatcc
     2041 cctggaagaa gggcgagccg gagcgctgga agaccgcctg ggacctcctg gtcacggaca
     2101 agggcggcct ggtctaccag cccatcaccg gtctgtacga gggcgtggcc gaaattgatt
     2161 tcgcctccat gtatccggcc atgatggtcc atcacaacat ctggcccgag acggtggact
     2221 gcgcctgctg cccggacaat gccgcccccg agatcggcta ctcgacctgc cggcggcgcg
     2281 agggcctggt caccaagacg ttgcgcccga tcctcgcgcg gcggcaggaa ttgaagcgcc
     2341 tccgcaacgc cgccgccggc ccggcccgcg cgcgctacga ccaccggcag accgccctca
     2401 agtggatcct ggtaacctgc ttcggctacc tcggctataa gaacgcccgc ttcgggcgga
     2461 tcgaggcgca cgagacggtg accgcctacg cccgagagaa gctgctcacc gcccgcaccc
     2521 tcgccgagga ccggggtttc cgggtactcc acgctatcgt ggactccatg tggatccaga
     2581 ggccgggggc gaccgagacg gaggtcctga acctctgccg ggccatccag gaggcggtgg
     2641 ggatcggcat caacctggag gggatctaca agtgggtgag cttcctaccc tcgcgcgtgg
     2701 acccggcggt ctccgtggcc aaccgctacc tgggtgcctt ccgttcgggc gagctgaagg
     2761 tgcgagggat cgacctgcgc cggtccgaca ccccgcccct cgtggccgag gcacagcagc
     2821 gcatgctcgc gatcctggcg caggcggacg atcttgccgg gttcgcagcg cgaatcccga
     2881 aggtactcga ggtcttcgtg gagtacgcct ggaggctccg agaggggcag gtgcgcctgg
     2941 aggccctcat catcaagcgc accgtctcca aagaggcgga cgagtacaag accaacagcc
     3001 agatcggtct ggccagtcgg caaatggccg accggggcgt agctgtccat cccggcgagc
     3061 gggtgcggta cgtgatcagc gacgccgcgg cgcccaacgc cgaagcgcgg gtccgggcct
     3121 tcccgctcgt cgccgccgac acatcctatg acaccgggca gtacctggga ctcttgtgca
     3181 aggccgtcga gacgctcctc tggcaccacg ggtatgactg ccggcgactc ctggacctgc
     3241 tcgctgaacg gaacgtcgtc gcagaccgct catcggccgg gcgaggccgc gcgtgagcat
     3301 ccatctccat tcgcgactat ctcttccccc tcgcccgaga cctccagaaa taaaaagggg
     3361 gaaacctggc tcaggtttcc ccctggttgc cgtacgggtc aggagtatga ccggctactg
     3421 gatcggctcg acgagaatcg ggtcacccca ccacttttcc tgttgtgccg aggcgacgtc
     3481 catcttggtg gtttccttgg gggtcgcccg gaacagggaa taccccatgt gctgccacgt
     3541 tgcgaaatgt gccccgttct ggcgctcctc ggccaagtac cggctccagc taccacatcc
     3601 cgacagcgaa accatgaccc cgatcgcgag cagtgcaacc cacgcccgtc tccgcatagc
     3661 agtatcctcc tttctggagc gcatcctagc gcggtacttc cggtgctgtc aaggggggcc
     3721 tcgtgggtcg ttcgaatccg accaaccggt cagccaggcg cgtggcccgg cggagcgtga
     3781 catcgttgcg taccgcgatg gccaaggctt tcttcgtccc caccaggacc aggagacgtc
     3841 gcgcgcgcgt gatggcggta tacagcaggt tccgttgcag catggggtag tgggtggtat
     3901 ggagggggag gatcaccgcg ggatactcgc atccctggct cttgtggacg gtgaccgcat
     3961 aggccagtgt gagctcgtcc aattcgctga agtcgtaggt cagctcccgg tcatccacct
     4021 gcacgaccac ggcctggtcg ggcgcgttga tcgacctgag gcgccccagg tccccattga
     4081 agacggcctt gtcataattg ttt
//
LOCUS       DBNGZC010000898         1736 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_467831, whole genome shotgun sequence.
ACCESSION   DBNGZC010000898 DBNGZC010000000
VERSION     DBNGZC010000898.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_467831"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="AC16G0_15565"
     CDS             complement(<1..1152)
                     /locus_tag="AC16G0_15565"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR5101072.1"
                     /translation="MTSWFRKPTGWGIVVGLIACLLVGCGRELPLEARVEQLVTKADY
                     ARALDLIAEALRKDPAAWPVQDLRVRVLLAAGRPEEALSAYATRWSQGGPDSPDLFRR
                     IATGLLKEGLESQDGLLRNRAAAALAGFATPEHLPVFREALKHPDASVRALSVQGLGH
                     MQAEESGRLLWEALQDPSAEVRAAAADALGMRADAESQAALARTLNDPSRKVRLRTVA
                     ALARQNDATAFASLRQYLGDPDDSIRMQAAEALGHLGNREAAPELRVLLRDPNPYVRV
                     YAAEALARLEDPAGREAMRAALRHPNRSVALYAAEAFSNLGDASGTPRLIDALRPDED
                     LSVRLYAAWTLARLGDFRGLPILEELLQHGQTQVRVQATWTLGEMQQGVG"
     gene            complement(1133..1483)
                     /locus_tag="AC16G0_15570"
     CDS             complement(1133..1483)
                     /locus_tag="AC16G0_15570"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HIR5101073.1"
                     /translation="MDDLVKAVLAFRDQRDWQQFHNPKDLAISVTLEAAELLEHFQWK
                     TAGEVAAFVAAQENRKRVGEEMADVLILLISLADAMGLDLLEAARAKLQENARKYPVD
                     RARGNAKKYDELVP"
     gene            complement(1494..>1736)
                     /locus_tag="AC16G0_15575"
     CDS             complement(1494..>1736)
                     /locus_tag="AC16G0_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HIR5101074.1"
                     /translation="LLHESLFRVVPPVCIDEAICGMFDEDPGALRGTARKRREDTLDA
                     VVCSLVGWIHWWHGGRRSEVLGDIETGFIVVPKRDV"
BASE COUNT          278 a          632 c          550 g          276 t
ORIGIN      
        1 gcccacaccc tgctgcatct cccccagcgt ccaggttgcc tggacgcgga cctgcgtctg
       61 cccgtgctgg agcagttcct ccaggatcgg cagaccccgg aaatccccca ggcgtgccaa
      121 agtccaggcg gcgtagagac gcaccgagag atcctcgtcg ggtctgaggg catcgatgag
      181 gcgtggggtc ccgctggcat caccgagatt cgagaaggcc tcggccgcat acagggccac
      241 cgaccggttg gggtgccgga gggccgcccg catcgcctcc cgaccggcgg ggtcctcgag
      301 acgcgccagc gcctcggcgg catagacacg gacataggga ttcggatctc gcagcagcac
      361 tcggagttcg ggcgccgcct cccggtttcc gagatggccc aacgcttccg ccgcctgcat
      421 gcggatggag tcgtctggat cgcccagata ctggcgcaga gaggcgaagg ccgtcgcgtc
      481 attctgccgt gccagagcgg cgaccgtccg caatctgacc ttgcggctgg gatcgttgag
      541 cgtccgggcg agcgccgcct gggattcggc gtcggcccgc atccccaatg cgtccgcggc
      601 ggcggctcgc acctcggcgc tcggatcctg cagggcctcc cacagcagtc gcccgctctc
      661 ctcagcctgc atgtgtccga gtccctggac ggacagggcg cggacggacg catccgggtg
      721 cttcagcgcc tcccggaaca cgggcagatg ctcgggagtg gcaaaccctg ccagcgcggc
      781 ggcggcgcgg tttcgcagga ggccatcctg gctttccagc ccctccttca gcaggccggt
      841 ggcgatccgg cggaacaggt ccggagagtc ggggcccccc tgcgaccacc gggttgcata
      901 ggccgagagc gcctcctcgg gccggccggc cgccagcagc acccggacac ggaggtcctg
      961 caccggccag gccgcgggat ccttccggag cgcctcggcg atgaggtcga gcgcccgggc
     1021 gtagtccgcc ttcgtcacga gctgctccac gcgcgcctcc agcgggagtt cccggccgca
     1081 gcccacgagg agacaggcga tcagcccgac gacgatcccc caccccgtcg gtttacggaa
     1141 ccagctcgtc atatttcttg gcgttgcctc tcgcacgatc taccggatat ttcctggcgt
     1201 tttcctgcag ctttgctcgg gccgcctcca ggaggtccag gcccatcgca tccgccaggg
     1261 agatcaggag aatgagcaca tcggccatct cttcaccgac acgcttccgg ttctcctggg
     1321 cggcgacgaa cgccgccacc tcgccggcag tcttccactg gaagtgctcc aatagctccg
     1381 ccgcctccag cgtgacggag atcgccaggt ctttgggatt atggaactgc tgccagtcgc
     1441 gctgatcgcg gaaggccagg actgctttca cgaggtcgtc catgcgtccc ccatcacacg
     1501 tcccttttcg gcacgacgat gaatcccgtc tcgatatcgc caagcacctc gctccgtcgc
     1561 ccgccgtgcc accagtgaat ccagcccacc agggaacaaa cgacggcgtc cagggtatcc
     1621 tcgcgccgct tgcgggcggt cccccggagc gcccccggat cctcgtcaaa catgccgcat
     1681 atcgcctcgt cgatacacac cggagggacc acccggaaca atgactcgtg cagcag
//
LOCUS       DBNGZC010000899         2859 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1738143, whole genome shotgun sequence.
ACCESSION   DBNGZC010000899 DBNGZC010000000
VERSION     DBNGZC010000899.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1738143"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..2225)
                     /gene="ligA"
                     /locus_tag="AC16G0_15580"
     CDS             complement(216..2225)
                     /gene="ligA"
                     /locus_tag="AC16G0_15580"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR5101075.1"
                     /translation="MATRKDLAGRAKELRALLQHHSHAYYVLDRPEISDAEYDRLFNQ
                     LKALEAAHPELVTPDSPTQRVGGAVAEGFASVEHRVAMLSLDNAYNADDIREFEGRLQ
                     RVLPGETFTYVCEPKVDGLGVALIYEKGLLVRGATRGDGRLGEDVTQNLKTVKTIPLR
                     LRGPLANLNVLEVRGEVFMSHAAFEKLNRALEAEGEEPFANPRNAAAGSLRQKDSSIT
                     AKRPLDISVYSLSDAEPNPFTTHWEAMQALGQGGFKYNPRAAKSPDIQAVIECCASLE
                     RDRPDLGYDADGVVIKVDSLDQQRRLGSTTHHPRWAVAYKFPAQQAITVIRRIEVSVG
                     RTGALTPTAHLDPVEIAGATISRATLHNADEIERLDVRERDTVVVERAGDVIPHILRV
                     VMDKRPPDAKRFAFPTTCPVCGSAAFRPEGEVVSRCTNAACPARLKEGLLHYGSRRAM
                     DIEHLGEAVVDQLVDRGLVRSYPDLYDLGVDQLAALDRLAEKSATNLHTAIQGSKARG
                     LARLLFALGIRYVGENVGRILATEFGSVQKLQEASEEQLAAVRGIGPRIAASVALFFR
                     QPENSRLVAALRQAGVKMEEESSAGPKPLLGKTFVLTGSLEGLTRDQAKEAIQRLGGR
                     LASSVSKKTDFVVVGQDPGSKYEDAKRLGLSLLDEAAFRKLLDQS"
     gene            2480..>2859
                     /locus_tag="AC16G0_15585"
     CDS             2480..>2859
                     /locus_tag="AC16G0_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101076.1"
                     /translation="MQSISATPSRAGQFAGPQARSAAFWVRRVRLTSGLVLLTYLVTH
                     FLNHALGLISLAAMETGRLWFLLLWRNPLASVILYGAGSIHFALALWSLYQRRTLRMP
                     VWEALQLTAGLAIPPLLVSHFVGTR"
BASE COUNT          448 a          919 c          968 g          524 t
ORIGIN      
        1 tgcacacctg cgaagcttgc ggagcgaagg ggtaacggaa ccggtgactc tagctagcag
       61 aatagcatgg cggaaagcct ctcgattgtc aaccgccgca tgcccgggaa atcgggcgga
      121 cgggtaactc ggcagcccgg aaatccgggg gtctgacaat cgagaggttc ggattggtgg
      181 gatgttggat tgagtaatct ccagggtggc aaaacttacg actggtccaa gagtttccgg
      241 aacgccgcct catccaagag cgagagcccg aggcgcttgg cgtcttcgta ctttgagccg
      301 gggtcttgcc ccacgaccac gaagtccgtc ttcttgctta ccgatgaggc gagcctgccg
      361 cccagccgct ggatggcctc cttggcctgg tcgcgggtca ggccttccag gctgccggtc
      421 aggacaaagg tcttgccgag gagcggcttg gggccggcgg aggactcttc ctccatcttg
      481 acccccgctt gccggagggc ggcgacgagg cggctattct ccggctggcg gaagaacaag
      541 gcgacgctgg ctgcgatacg cggcccgatg ccgcggacag cggccagctg ctcttccgaa
      601 gcctcctgca gtttctgcac gctcccgaat tccgtcgcca ggatccggcc cacgttctcc
      661 cccacatagc ggatccccag ggcgaacagg agcctggcca gaccccgggc cttgctgccc
      721 tggatggccg tgtgcaggtt ggtcgccgat ttctccgcga gccggtcgag ggctgcaagc
      781 tgatccacgc cgaggtcgta caggtcggga taggaccgga ccagaccccg gtccacgagc
      841 tgatccacca cggcctctcc caggtgctcg atgtccatgg cccggcggct gccgtaatgc
      901 aggaggccct ccttaagccg ggcggggcag gcggcgttgg tgcagcgtga aaccacttcg
      961 ccctcggggc ggaaggcggc ggacccgcag accgggcagg tggtgggaaa ggcgaagcgc
     1021 ttcgcatccg gcgggcgctt gtccatgacg acccggagga tgtggggaat cacgtccccg
     1081 gcacgctcga ccaccacggt gtccctctcc cggacatcca gccgctcgat ctcgtcggcg
     1141 ttgtgcagcg tcgcacggct gatggtggcg ccggcgatct ccaccggatc cagatgggcc
     1201 gtaggggtga gggcgcccgt ccgacccacg ctgacctcga tcctgcggat gaccgtgatt
     1261 gcctgctggg cggggaactt gtacgccacg gcccagcggg gatggtgggt ggtggagccc
     1321 aggcggcgtt gctggtcgag ggagtccacc ttgatcacca ccccgtcggc atcgtacccg
     1381 aggtcgggac ggtcccgctc cagcgacgcg cagcactcga tgacggcctg gatgtcaggg
     1441 gacttggctg cccgcggatt gtacttgaag cctccttggc cgagggcctg catggcctcc
     1501 cagtgcgtgg tgaagggatt gggctccgcg tcgctcaggc tatagacgga aatgtccaag
     1561 ggccgcttcg ccgttatgct ggagtctttc tgtcgcaggg atccggcggc ggcgttccgg
     1621 gggttggcga agggctcttc accttccgcc tccagggctc ggttcagctt ctcgaaggcg
     1681 gcgtgggaca tgaaaacctc gccccggacc tccaggacgt tcaggtttgc cagcgggccc
     1741 cgcagtcgga gcggaatggt cttgaccgtc ttcaggttct gcgtgacgtc ttcccccaga
     1801 cggccatctc cgcgagttgc gccccggacg agcaaacctt tctcgtagat cagggcgacg
     1861 cccagcccgt ccaccttcgg ctcgcacacg taggtgaagg tctcgcccgg aagaacgcgc
     1921 tgcagcctcc cctcgaactc ccggatgtcg tcggcgttgt aggcattgtc caggctgagc
     1981 atggccacgc ggtgctctac cgaggcaaag ccttccgcca ctgcgccccc cacgcgctgt
     2041 gtgggggagt cgggggtgac gagctcgggg tgcgccgcct ccagggcctt gagttggttg
     2101 aagagacggt cgtactcggc gtcgctgatc tcgggccggt cgaggacgta gtaggcgtgg
     2161 ctgtgatgct gcaggagcgc gcgaagctcc ttggcccgcc cggcgagatc cttcctggtg
     2221 gccatggttc cctccttcac ctgggttgta gcagaaggag gggtgcttga caagcagggg
     2281 acccagaccg atttcggaaa agcattgacg ggcttgggtt gaccggctat caaggcggaa
     2341 cgtcggggct ggggtcgggc cgacagtgat ggcgacaggg acgagggtat cgcagtcgga
     2401 tgtccgatga gaccgaccgc tggcgatcga ggtcaggctg gtgtattctc tggtctgaca
     2461 gccccagacc tggtggatca tgcagagcat cagcgccact ccgagtcggg ccggacagtt
     2521 cgccggcccc caagcgcgct cggccgcatt ctgggtccga cgggtacgct tgacctccgg
     2581 cctcgtcctg ctgacctatc tagtcacgca cttcctgaat cacgccctcg gcctgatctc
     2641 cctggccgcc atggagaccg gtcgcctctg gttcctcctc ctgtggcgga accccctggc
     2701 cagcgtcatc ctctacggcg cgggatcgat ccacttcgcc ctggcgctgt ggtccctcta
     2761 ccagcggcga accctccgaa tgccggtctg ggaggctctc cagctgacgg cggggctggc
     2821 gatccccccg ctcctggttt cccactttgt tggaacccg
//
LOCUS       DBNGZC010000900         2391 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1940306, whole genome shotgun sequence.
ACCESSION   DBNGZC010000900 DBNGZC010000000
VERSION     DBNGZC010000900.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1940306"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(370..1791)
                     /locus_tag="AC16G0_15590"
     CDS             complement(370..1791)
                     /locus_tag="AC16G0_15590"
                     /EC_number="5.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF021387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase family
                     protein"
                     /protein_id="HIR5101077.1"
                     /translation="MKPEVARIIRDADVAGWKRVDVEYGITTLQIQVPGSCRVLEMQE
                     VPPLADPRAAMRGSIHRPVGSRPLPEILRGKGRPVGELTVCITTSDITRPVPYKGEAG
                     ILQPLLELLHQCGIRKEHITLLVGTGTHRPSTPAEKAAMFGEDVVAQYRIVDHECDND
                     GLLVDIGKTRTGTEIHINKCFYQADVKIATALVESHFMAGASGGRKAICPALVSRKTI
                     EKFHSAEFLDSPLATNLVLEGNPCHEESLEIAKKAGVDFIINTVLNRRLELLDVFSGD
                     LVQAHAKAVELLKRIVAVPVEQEFDVVLTHGGYVGINHYQNAKAAVNALPAVKEHGFI
                     ILAACERDVDPVGPMTYKTLLHLLKLQGPDGYMSTLLNPGWTFTKDQWEPQMWAKVLK
                     KIGEEGMIYCSAVLSEKEHQLVPGGMGWDYLSEGESTDDAEKTRDMVQNAVIYAMHHP
                     RWGGSQPRMAFLKEGPYAVPVLK"
     gene            complement(1838..>2391)
                     /locus_tag="AC16G0_15595"
     CDS             complement(1838..>2391)
                     /locus_tag="AC16G0_15595"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5101078.1"
                     /translation="RPQRGEILLEKEPIHIVTPYEIVRKKIAMVPEGRQLFGRLSVLD
                     NLLLGAYALASDQEAQDSLERVMTLFPVLRERQAQRAETLSGGEQQQLAIGRGLMSRP
                     ALLMLDEPSLGIMPKLVSEIFRTIHEISQKGVTVFLVEQNVFEALNISNRAYVLQTGR
                     IVLEGKGADLLQSDLVRKAYLGM"
BASE COUNT          393 a          816 c          734 g          448 t
ORIGIN      
        1 catcctacct ccaagtcgcc cagacgtacc ttcggcaggt ggccgcgggt aaggcggacc
       61 tcacccggcg agaccgggaa gcctgccgca cgctcttacg ccgcctcgcg cagtcggagc
      121 agcttgagtt gccgtttgcc gagcccccct catccttcac ccgtgagcac acccggctcc
      181 tggcccgatc ctgggcaaag acctcggcgg cccggtcggc tacgacgggc tcgcctcgcg
      241 gcccttctgg caaaccaacc tgcgtgaggt cccggcggat cgctgggaat ccagcccagg
      301 gcacggattc ccggcgaagc aacggggaga ggaaacgggg ccgcccgagg agcaacagag
      361 ggaggggtcc tatttgagga ccggtacggc gtagggcccc tctttgagga aagccatgcg
      421 aggctggctg ccgccccatc tggggtggtg catcgcatag atgactgcgt tctgcaccat
      481 gtccctggtc ttctcggcgt cgtctgtgga ctccccctcc gacagataat cccagcccat
      541 gccgcccggg acgagctgat gctccttctc cgagaggacg gccgagcagt agatcatgcc
      601 ctcctccccg atcttcttca ggaccttggc ccacatctga ggctcccact ggtccttggt
      661 gaacgtccac ccgggattca gcagcgtgga catgtatccg tccggcccct gaagcttcaa
      721 gaggtggaga agcgtcttgt acgtcatcgg cccgacgggg tccacatccc gctcgcaggc
      781 ggccaggatg atgaagccgt gctccttgac ggccggcagc gcgttcacgg ccgccttggc
      841 gttctggtaa tgattgatcc cgacgtatcc cccgtgcgtc aagaccacgt cgaattcctg
      901 ctcgaccggg acggcgacga tgcgcttgag cagctcgacc gccttcgcgt gggcctgcac
      961 cagatccccg gagaagacgt cgagcagttc cagcctgcgg ttcagcaccg tgttgatgat
     1021 gaagtccacc ccggccttct tggcgatctc gagggactcc tcgtggcagg ggttgccttc
     1081 cagcaccaag ttcgtggcga ggggcgaatc caggaactcc gcgctgtgga atttctcgat
     1141 ggtcttccgg ctgaccaggg cgggacagat cgccttcctg ccgccggagg cccccgccat
     1201 gaagtggctc tccaccagcg ccgtggcgat cttgacgtcc gcctgataga agcacttgtt
     1261 gatgtggatc tcggtgccgg tcctggtctt cccgatgtcc accagcaggc cgtcgttgtc
     1321 gcactcgtga tcgacgatcc ggtactgcgc caccacgtcc tcgccgaaca tggcagcctt
     1381 ttccgccggg gtgctcggcc gatgggtgcc cgttccgacc aggagcgtga tgtgctcttt
     1441 ccggatgccg cattgatgca gcaattccag gaggggctgg aggattcccg cctcgccctt
     1501 gtacggcacg ggccgggtga tgtcggaggt ggtgatgcaa accgtcagct ctccaaccgg
     1561 cctccccttg cccctcagga tctccggcag cggcctggag ccgacggggc ggtgaatgga
     1621 gccgcgcatc gccgcccgcg gatccgccag gggtgggacc tcttgcatct ccaggacccg
     1681 gcagctcccg ggcacctgga tctgcagggt cgtgatcccg tactccacgt ccacgcgctt
     1741 ccagccggcc acatcggcgt cgcggatgat gcgtgccacc tcaggcttca ctgcagaccc
     1801 ctccccgccg gccgggttcc cgttcgtgaa atgccgccta cattcccagg taagccttcc
     1861 ggaccaggtc tgattgtagc agatcagccc ccttgccttc caggacgatc cgcccggtct
     1921 gaagcacgta cgcccggttg gagatattca gggcctcgaa gacgttctgc tcgacgagaa
     1981 agacggtgac gcccttctgg ctgatctcgt ggatcgtcct gaagatctcc gacaccagct
     2041 tcggcatgat tccgagggac ggttcatcca acatcaggag ggccgggcgc gacatcaaac
     2101 cgcggccgat ggccagttgt tgctgctccc cgccactcaa ggtctccgcg cgctgcgcct
     2161 gcctctcgcg gagcaccggg aagagggtca tcaccctctc cagcgagtcc tgggcctcct
     2221 ggtcggaagc caaggcatag gccccgagca ggagattgtc gagcaccgac aatcgtccga
     2281 agagctgccg tccctccgga accatggcga tcttcttccg cacgatctcg tagggcgtga
     2341 cgatgtggat cggctccttc tccagcagga tctcccctct ctggggacgc a
//
LOCUS       DBNGZC010000901         1747 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1447500, whole genome shotgun sequence.
ACCESSION   DBNGZC010000901 DBNGZC010000000
VERSION     DBNGZC010000901.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1447500"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1747
                     /locus_tag="AC16G0_15600"
     CDS             <1..>1747
                     /locus_tag="AC16G0_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5101079.1"
                     /translation="VAYRNGAPVRLGELGQVIDSVQNDKVASWYNNRRGIVLAIQRQP
                     GTNTIEVVDAIKALLPKLQAQIPPSVNVNVLYDRSVSIRESIHDVQFTLMLTICLVVL
                     VIFLFLRNLSATVIPSLAVPMSIVGTFGVMYLLGYTIDNLSLMALTLSVGFVVDDAIV
                     MLENIVRHMEEGKGRLEAALDGAKEIGFTILSMTLSLAAVFIPVLFMRGVVGRLLHEF
                     AVTIGVAVLVSGFISLTLTPMACSRFLRPPTESHGRLYVLSERVFEGMRDLYAWTLEY
                     VMAHPRFTALTLLATIGLTAYLFVVIPKGFIPNEDTGQIFAFTEASQDVSFEAMREHQ
                     QEAATIVARNPYINNFMSSIGAGGPNLVPNTGRMMIRLKPRSMRPDVNRIIADLRPKL
                     AAVPGLKVYPQVVPSIRIGGQLTKALYQYSLQDADLTELYHWAPILYDKARALPGFSD
                     VNTDLQLTSPQAIVEIDRDQATALGVTAGQIESALGDAYGSQQVSTIYTPTNQYWVIL
                     ELQPQYQQDPASLSLLYIRSSKGQLVSLDTVTKLTQGVGPLTVNHVGQLPSVTISFNL
                     VPGVSLGQAVTSLQKV"
BASE COUNT          306 a          599 c          499 g          343 t
ORIGIN      
        1 gtggcctacc ggaacggggc tcctgtgcgg ctgggggagc tgggccaagt catcgacagc
       61 gtacagaacg acaaggtggc gagctggtat aacaaccgcc gcggcatcgt gctggccatc
      121 cagcggcagc ccggcaccaa caccatcgaa gtggtggacg ccatcaaggc gctgctcccc
      181 aagctccagg cgcagatccc accctcggta aacgtgaacg tcctctacga ccggtccgtt
      241 tcgatccgcg agtcgattca cgacgtccag ttcaccctga tgctgacgat ctgcttggtt
      301 gtgctggtga tcttcctctt cctgcgtaac ctctccgcca cggtcatccc cagcctcgcc
      361 gtccccatgt ccatcgtcgg caccttcggg gtcatgtacc ttctgggcta cacgatcgac
      421 aatctgtcgc tgatggccct gaccctttcg gtcggcttcg tggtggatga cgccatcgtg
      481 atgctcgaga acatcgtccg ccacatggag gagggcaagg gtcgcctcga ggcagcgctg
      541 gacggcgcca aggagatcgg cttcaccatc ctttccatga cgctgtccct ggcagctgtc
      601 ttcattccag tgctcttcat gcggggtgtg gtggggcggc tgctgcacga gttcgccgtc
      661 accatcggcg tcgccgtgct ggtgtccggg ttcatctcct tgaccctcac ccctatggcc
      721 tgcagtcgtt tcctgcggcc gccgacggaa tcgcacggtc gcctctacgt cctctcggag
      781 cgagtgttcg aggggatgcg cgatctttac gcgtggacgc tggagtacgt catggcgcat
      841 ccccggttca ccgcgctgac cctcctcgcc accatcgggc tcaccgccta cctgttcgtg
      901 gtgataccca agggcttcat tcccaacgag gacacggggc agatcttcgc cttcacagag
      961 gcgtcccagg atgtctcctt cgaggcgatg cgggagcatc agcaggaggc ggcaaccatc
     1021 gtggcgcgga atccgtacat caacaacttc atgtcgtcca tcggcgcggg ggggccaaac
     1081 ctggtcccga atacgggccg gatgatgatc cgcctcaagc cgaggagcat gcgaccggat
     1141 gtgaaccgga tcatcgccga cctccgaccc aaactggctg cggtgcccgg cctcaaagtc
     1201 tatccccagg tggttcccag catccggatc ggcggccagt tgaccaaggc cttgtaccag
     1261 tactccctgc aagatgcgga tctgacggag ctctatcatt gggccccgat cctctacgac
     1321 aaggcccgcg cgttgccagg attctcggac gtcaacaccg acctccagct caccagcccc
     1381 caggcgattg tcgagatcga ccgcgaccag gccaccgccc tcggggtcac ggcaggccag
     1441 atcgagagcg ccctgggtga tgcctacggc tcgcagcagg tttccactat ctacaccccc
     1501 acgaaccagt actgggtcat cctggagctg cagccgcagt accagcaaga ccccgcctcc
     1561 ctctccctgc tgtacatccg ctcctcgaaa ggacagttgg tatcgctcga caccgtgaca
     1621 aagctgaccc agggcgtcgg tccgctcacg gtcaatcacg tcggccagct tccctccgtg
     1681 accatctcct tcaaccttgt tccaggcgtg tccctcgggc aggcggtgac ttctcttcag
     1741 aaggtcc
//
LOCUS       DBNGZC010000902         3216 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_272784, whole genome shotgun sequence.
ACCESSION   DBNGZC010000902 DBNGZC010000000
VERSION     DBNGZC010000902.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_272784"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="AC16G0_15605"
     CDS             complement(<1..286)
                     /locus_tag="AC16G0_15605"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR5101080.1"
                     /translation="MNREANWPLAGFTALSPLSIGGLAGLLMVRGTPRDAADNGATAL
                     LGIALLALLASALHLGRPLRAYRSIARLSTSWLSREIILFGIFVLLLAAYA"
     gene            complement(288..1472)
                     /locus_tag="AC16G0_15610"
     CDS             complement(288..1472)
                     /locus_tag="AC16G0_15610"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR5101081.1"
                     /translation="MSVEVRVPQVTQTMSECTITRWLKHEGETVEAGEPLFEMETDKA
                     AVEVESPGAGRLAKILVASGAAPVGGVVAVILTPGENLSEPVYSPATARTSPRAVATA
                     GQPPSPLGREAGPTGRLRVSPPAKRLALEHGVDLSSVVPTGPGGLITEGDVQRYLKTA
                     PVAPALTEGEEIIPLIGVRKTIADRLSLSRRTAADVTTVAEIDMTEVAALREHEDITY
                     TAFVAAATVMALAEFPILNSRLQDDKIIVSKRVHLGVAVALDEGLKVPVIRDAHTKGL
                     RQIAAELADLARRGAAGQLAPAELTGSTFTLTNSGVFGAVLFTPIINPPESAILGMGR
                     IAKTPVVLDDRIVIRKMMYLCLTYDHRVVDGAPAVKFLQRMKLLLEVPEPMLSSESHG
                     RP"
     gene            complement(1485..2468)
                     /locus_tag="AC16G0_15615"
     CDS             complement(1485..2468)
                     /locus_tag="AC16G0_15615"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019124009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR5101082.1"
                     /translation="MRELTFREALREALQDEMRQDSSVFLLGEDIGRHGGAHKVTEGL
                     LDEFGANRVMDMPISESAIVGAAVGAAIMGMRPVAEIMFADFLTVAMDQIVNNAAKMC
                     YIHDGEISVPMVVRGNCGAGTRMGVHHGQSFEAWFAHVPGLKVVMPSTPADAKGLMKS
                     AIRDCNPVIFHEHKLLYGTKGPVPEGDYLVPLGKADVKREGADATVVATGVMVNRALG
                     AAKKLQEEGISVEVVDVRTIQPLDTETIVASACKTGRVIIAQEAPGPFSAASEIAAVI
                     GREAFGYLDMPIEMVCEPFVPVPFSPVLEDFVIPKEGQIISAVRRVLRREA"
     gene            complement(2468..>3216)
                     /locus_tag="AC16G0_15620"
     CDS             complement(2468..>3216)
                     /locus_tag="AC16G0_15620"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR5101083.1"
                     /translation="MAAIYGKATGTARGKETSHHMGDKALGLVAGSGVVGGSIAVAVG
                     TALACKLLSTSRVTLCFFGDGASNRGDFHESLNLAAIQKVPGVFICENNQYALSTPAS
                     YHLPIANVADRAASYGIPGVVADGNDVMAVHDVVQQAVARARGQEGPTLIECKTYRWR
                     GHSERDLKQGSYYRTSEEVEEWKARCPIKRLRELLLSRRALSDESVRLIDEEMIQVVD
                     KAVAFAEESPYPAPEEALENVYATQDGSFR"
BASE COUNT          538 a         1097 c          988 g          593 t
ORIGIN      
        1 gggcgtaggc cgccagcaga agaacaaaga tgccgaacag gatgatctcc cggctgagcc
       61 aggaggtaga gagccgcgcg atggatcggt aggcgcggag cggacgcccg aggtgcaacg
      121 cagaggcgag cagggcaagc agggcaattc ccaacaatgc tgtggcaccg ttatccgcgg
      181 catctctggg ggtcccccga accatcagca gacccgccag gccaccgatg ctaagcggcg
      241 ataacgcggt gaaccccgca agtggccagt tcgcctcgcg attcacctca cggcctcccg
      301 tgggattccg acgacagcat aggttccggc acctccagga ggagcttcat ccgctgcaga
      361 aacttgaccg ccggcgcgcc gtcgacgacc cggtggtcat aggtcaggca gagatacatc
      421 atcttgcgga tgacgatccg atcgtccagg actacgggtg tcttggcgat tcttcccatg
      481 cccaggattg cgctttcggg agggttgatg atgggcgtga acaggaccgc cccgaagacc
      541 ccggaattgg tgagggtgaa ggtgctgccc gtgagttccg ctggtgcgag ctggcctgcc
      601 gctccgcgcc tggccaggtc cgccaactca gcagcgatct gcctgaggcc tttggtgtgg
      661 gcgtcccgga tcaccggcac cttcaatccc tcgtccagag cgaccgccac ccccaagtgg
      721 acccgcttgc tgacgatgat cttgtcgtcc tggagtctgg agttcaggat gggaaactcc
      781 gccagcgcca tgaccgtggc cgcggccacg aaggcggtat aggtgatgtc ctcatgctcc
      841 cgtagcgcag cgacttcggt catatcgatt tctgcgacgg tggtcacatc cgcggcggtc
      901 cgtcggctga gggagagtcg gtcggcaatg gtcttccgga cgccaatcaa cggtatgatt
      961 tcctcgcctt ccgttagcgc cggtgcgacg ggcgcggtct tcaggtaacg ctggacatct
     1021 ccctcggtga tcaggccgcc gggtccggtt ggaacgaccg aactcaggtc gacgccatgc
     1081 tccaaggcca gcctcttggc cggcggcgac accctcaagc gcccggtcgg accagcctcc
     1141 ctgccgagag gggagggagg ctgcccggct gttgccacgg ctcgcggaga ggtccgcgcc
     1201 gttgccggcg aatagacggg ctccgagagg ttctcgcccg gcgtcaggat gacggcaacc
     1261 accccgccca caggagccgc cccgctggcg accaggatct tggccagcct gcccgccccg
     1321 ggcgattcga cctccacggc cgccttgtcc gtctccatct cgaacagggg ctcgcccgcc
     1381 tcgaccgtct caccctcgtg cttcagccac cgggtgatgg tgcattcgga catcgtctgg
     1441 gtcacttgtg gcactcggac ttcgacgctc atgggtcgat cccctcaagc ttcccgcctc
     1501 aggacccgcc tcacggccga gatgatctgc ccctcctttg ggatgacgaa atcctccagg
     1561 acgggagaaa aaggcaccgg cacgaagggt tcgcacacca tttcgatcgg catgtcgagg
     1621 taaccgaagg cctctctgcc gatgaccgca gcgatctcgg aagccgcgct gaagggcccc
     1681 ggggcctcct gggcgatgat gacacggccg gtcttgcagg cggacgccac gatggtctcg
     1741 gtgtccagcg gctggatcgt ccggacgtca accacctcca ccgagatccc ctcctcctgc
     1801 agtttcttgg cggctccgag ggctctattc accatgacgc ccgtggccac cacggtggca
     1861 tcagcgcctt cccgcttgac gtcggccttt ccgagcggca ctaggtagtc accttccggc
     1921 acgggtccct tggtgccgta cagcaacttg tgctcgtgga agatcaccgg attgcagtcc
     1981 cggatggcgg atttcatcag ccccttggcg tccgcgggcg tcgagggcat gaccaccttg
     2041 agtcccggga cgtgggcaaa ccaggcctca aagctctggc catggtgcac acccatgcgg
     2101 gtcccggcgc cgcagtttcc ccggaccacc atggggacgc tgatctcgcc gtcatggatg
     2161 tagcacatct ttgcagcatt attcacaatc tggtccatgg cgacagtcag gaagtccgca
     2221 aacatgatct cggccaccgg acgcatcccc atgatggccg cgccaaccgc ggctccgacg
     2281 atggcggact ccgagatggg catgtccatg acccggttgg cgccgaactc gtccagcagc
     2341 ccttccgtga ccttgtgcgc accgccgtgc cgtccgatgt cctcgcccag caagaatacg
     2401 ctgctgtcct gccgcatctc atcctggagc gcttcgcgca gtgcctctct gaatgttagc
     2461 tctctcatca tcgaaagctc ccatcctgtg ttgcgtacac gttctccaag gcctcctcgg
     2521 gcgccggata tgggctctct tctgcaaacg ccaccgcctt gtcgacgacc tggatcatct
     2581 cttcgtcgat gagacgcacg ctctcgtcgc tcagggccct ccgggagagg agcagctcac
     2641 ggagacgctt gatcgggcag cgtgccttcc actcctcgac ctcctcgctg gtccggtagt
     2701 aactcccctg cttcaggtct cgctccgagt gcccccgcca ccggtaggtc ttgcactcga
     2761 tcagggtggg cccttcctgc ccccgcgccc gggcgacggc ctgctggacg acatcgtgca
     2821 cggccatgac gtcgttgccg tccgcgacca caccaggtat gccatagctc gctgctcggt
     2881 ccgccacgtt cgcgatgggg aggtgatagg acgccggcgt ggacagggcg tactggttgt
     2941 tctcgcagat gaataccccc ggaaccttct ggatcgctgc caggttcagc gactcgtgga
     3001 agtcgccgcg gttcgaggcg ccgtcaccga agaagcacaa cgtgacccgg ctagtgctga
     3061 ggagcttgca agcgagtgcc gtgcccaccg ccaccgcgat ggaccctccc accactccgc
     3121 ttcccgccac caggccgagc gctttgtctc ccatgtggtg gctggtctcc ttgccgcggg
     3181 cggttcccgt cgccttgccg tagatcgcgg ccatgg
//
LOCUS       DBNGZC010000903         2388 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2621844, whole genome shotgun sequence.
ACCESSION   DBNGZC010000903 DBNGZC010000000
VERSION     DBNGZC010000903.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2621844"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2388)
                     /locus_tag="AC16G0_15625"
     CDS             complement(<1..>2388)
                     /locus_tag="AC16G0_15625"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR5101084.1"
                     /translation="QARDVRGSRFAAAQQAALAAVDSLRPGQRGMVIEAGQEARVLVP
                     FTEDRRALRQAIQTLQPLDVPGRISEAFRLAQANLLVGGGQAAVEVFTDGAFELSGLP
                     DLGPTAIHWRRYAQQGHNIGITAFEVRKTYFGSFDYQAFLSVANFSREPTSFDLTLTL
                     DGRSLKTERVNLEPEVKRSIVLPFTHDGGGILRAEVSTEDDLAADNHAFAVIPAPHLV
                     RVLLLSPGNAFLEKALAADPQVQVETGTVEQFGKKAGEYDVVILDRVPVKTVPAGRYL
                     FINTLPEGTSLKLQGRVEDPPIMDWDRTHPVLRYLDLSKVVVQDAMRVRPMGSGRALV
                     DSNLTPLIYALDEGGLKAILVGFDLYRTDFPLRVAFPLFVSNALRWLYPSQLEDAGLQ
                     LSTGQPLTVGLPAGSGVATVVDPTGKSHRLRPDADGRISFVETTHAGIYTVKAGTWEQ
                     RFAVNLLDEGESNIAPRAQFPAERASQAPSEDHPRTFPSRQDLWRVFAALALLLLLFE
                     GVLYYREMQGRWPLPAFGVRALVLLLVVTALAGVGLRRVTDQLNVLFLIDASDSVPLE
                     TRVKARQYLQDALKAAGPRDQFGVVIFGGSPVLETPVGTGALETKPPIVSESQSTDIG
                     AAIKLALAAFPREGAKRIVLLSDGNENEGSAREAAQRAQSEGADIDVVPLRSDHPAEA
                     LVERLVVPQEVKFGESFLVRIVAWSAKQASGRLSLYRDGEFVGAQPVKLTPGKNVFSY
                     QQSVDQGGFHIFQARLETPDDPQQENKRGVGVVAVRGRPRVLYVEKDRDQGANLL"
BASE COUNT          397 a          802 c          793 g          396 t
ORIGIN      
        1 ggaggaggtt cgcaccctga tcgcggtcct tctctacgta cagcacgcgt ggccgtcccc
       61 ggacagcgac cacccccacg ccccgcttgt tctcctgctg cgggtcatcg ggcgtctcaa
      121 ggcgggcctg gaagatgtgg aagccgccct ggtcgacgga ctgctgataa ctgaagacgt
      181 tcttgcccgg ggtgagcttc accggctggg cgcccacgaa ctcaccatct cgatacaagg
      241 agagtcgccc agaggcctgc ttcgcgctcc aggcgacaat gcgcaccagg aaggactcgc
      301 cgaacttcac ctcctggggg accaccagtc gctccaccag ggcctctgcc ggatgatcgc
      361 tccgcagggg gacgacgtcg atgtccgccc cctcggactg cgcccgctgg gccgcctccc
      421 gcgcgctccc ctcgttctcg ttgccgtcgc tcagcagcac gatccgcttt gcgccctctc
      481 tggggaaggc ggccagggcc agcttgatgg ccgcgccgat gtcggtggat tggctctcgc
      541 tcacgatggg tggcttcgtc tccagggcac ccgttcccac gggcgtctcc aagaccgggc
      601 tgccgccgaa gatcaccacc ccgaattggt cgcgggggcc cgcagccttc agcgcgtcct
      661 gcaggtactg ccgcgccttc acccgcgtct ccagaggcac gctgtccgag gcatcgatca
      721 ggaagaggac gttgagctgg tccgtgactc gtctgagtcc gaccccagcc agcgcggtga
      781 cgaccaggag gaggacgagg gcgcggacgc cgaaggccgg cagaggccag cgaccctgca
      841 tctctcgata gtacagcacg ccctcgaaga gcagcaggag gagggccagc gcggcgaaga
      901 cccgccagag gtcctggcgc gagggaaacg tgcgggggtg gtcctcgctc ggtgcctggc
      961 tggcccgctc ggcggggaac tgggcccgcg gcgcaatgtt ggactctccc tcgtccagga
     1021 gattcacggc gaagcgctgc tcccacgtcc ccgccttcac cgtgtagatg ccggcgtggg
     1081 tcgtctcaac aaaactgatc cgcccgtccg cgtccggtct cagccggtgg ctcttgcccg
     1141 tggggtctac gaccgtggcc accccagacc cggccggcag acctaccgtc agaggctggc
     1201 cggtcgagag ctgcagaccc gcgtcctcca gctgactcgg atacagccag cgcagggcgt
     1261 tgctcacgaa cagggggaat gccactcgca ggggaaaatc ggtccggtac agatcgaagc
     1321 cgacgaggat ggccttcagc ccaccttcgt ccagggcgta gatcaggggg gtgagattgg
     1381 aatcgaccag cgcacgtccg ctccccatgg ggcgaacgcg catggcatcc tggaccacca
     1441 ctttcgagag atcgaggtag cggaggacgg gatgggttcg atcccagtcc atgatgggag
     1501 ggtcttcaac ccggccctgg agtttgagcg aggttccctc cggcagggtg ttgatgaaga
     1561 ggtagcgacc ggcgggcacc gtcttcaccg gcacgcggtc caggatcacc acgtcgtact
     1621 ctcccgcttt cttcccgaac tgctccacgg tcccggtttc cacctggacc tgggggtcgg
     1681 cggccagcgc cttctccaag aaggcgttcc ccggactcag caggagcacc cgcacgagat
     1741 gcggtgcggg aattaccgcg aaggcgtggt tgtccgccgc caggtcgtcc tcggtgctga
     1801 cctccgcccg gaggatccca cccccgtcgt gggtgaaggg gagaacgatt gagcgcttga
     1861 cctcgggttc caggtttact cgttccgtct tcagcgatct cccgtcgagg gtgagcgtga
     1921 gatcaaagct ggtcggctcg cggctgaaat tcgcaacgga gagaaacgcc tggtaatcga
     1981 aggacccgaa gtaggtcttg cgaacctcga atgcggtgat gccgatgttg tgtccttgtt
     2041 gcgcgtagcg ccgccagtga atggccgtcg gccccaggtc ggggaggcct gacagctcga
     2101 aggctccatc ggtgaagact tccaccgccg cctgcccccc tccaaccagg aggttggcct
     2161 gggccagacg gaacgcctcg gaaatccgac cggggacgtc cagcggctga agcgtctgga
     2221 tggcttggcg cagagcacgt cggtcctcgg taaaggggac caggactcgc gcctcttggc
     2281 cggcttcgat gaccatgccc cgctgtccgg gccggagact gtccaccgca gccagagcgg
     2341 cctgctgggc tgcggcgaag cgcgaccccc ggacgtcccg ggcttgca
//
LOCUS       DBNGZC010000904         5723 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1543555, whole genome shotgun sequence.
ACCESSION   DBNGZC010000904 DBNGZC010000000
VERSION     DBNGZC010000904.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1543555"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="AC16G0_15630"
     CDS             <1..1020
                     /locus_tag="AC16G0_15630"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HIR5101085.1"
                     /translation="KARGMNPLGGHDEREQTLNQLLVEMDGFETNKGVIIMAATNRPE
                     ILDPALLRPGRFDRQVLVDRPDLKGREAILRVHAKGVTMAPQVELAAVAAKTPGFVGA
                     DLANIVNEAALLAARQGKDAVGMADFEEAIDRVVAGLKKTRVMSVKEREIVATHEAGH
                     AVAAASLPGTDPVAKVSIISRGIAALGYTQQLPTEDRYLMTRSELCNRLCVLLGGRTA
                     EEIVFGDVSTGAQNDLQRATDLVRHMVAQYGMSEKVGLMTFDGAGRSGLLPGLAPPRE
                     YSEETARLIDAEASRLLIEAHVKVSALLTEKRSLVEQLARRLLEKEVVERDELQALLA
                     GATLS"
     gene            1084..1284
                     /locus_tag="AC16G0_15635"
     CDS             1084..1284
                     /locus_tag="AC16G0_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101086.1"
                     /translation="MDEERSSYPYLRYTITTLRAEGVWWARARVAEKDAGGDRPVLGG
                     PWESKPEANTAARAFCDSGKAG"
     gene            1538..1858
                     /locus_tag="AC16G0_15640"
     CDS             1538..1858
                     /locus_tag="AC16G0_15640"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HIR5101087.1"
                     /translation="MVVSTLRIVTVPQSRAEVVRTLAAQLGPTRVQPGCLRCDLYRDV
                     EDRGAITLVEEWESQADLDLRLRSEDYRSVLAAIELAREPPVIHFDTVTRRAGLEIVA
                     SARG"
     gene            1952..3022
                     /locus_tag="AC16G0_15645"
     CDS             1952..3022
                     /locus_tag="AC16G0_15645"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HIR5101088.1"
                     /translation="MNFQARRADGSSDRFAPTTTLQPAKSMWKRIVARYEKPALSRSA
                     WQAANTIVSYGVLWCLMYRSVAISYWITLALAIPTAGFFVRLFIIFHDCGHGSFFQSR
                     KANEALGIFTGILVFTPYYQWRHKHAIHHATSGDLDRRGTGDVWTMTVREYLEASRWR
                     RFAYRLVRNPAVLLGIIPLLLLVVEHRFPWRKADRRERSNVWWTNLAILGMAILLSRL
                     MGLQAYLAIQATVLMIAGSVGVWLFYVQHQFDGAYWEHRDRWDYVIAALHGSSFYKLP
                     RVLQWFTGNIGFHHIHHLSPGIPNYNLEKCYRADPLFQRVARVTLLSSLKALTYRLWD
                     EERRVWVGYRHLKALRDGRTRD"
     gene            3132..3425
                     /locus_tag="AC16G0_15650"
     CDS             3132..3425
                     /locus_tag="AC16G0_15650"
                     /inference="COORDINATES: protein motif:HMM:NF012305.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR5101089.1"
                     /translation="MGTRLYVGNLPFDVDESQLRPLFQEGGRGVTEVKMVVDRETGRP
                     RGFAFVEMASQADAQAAISALNGRELGGRALTVNEARERVPRSGGFGGGRSSH"
     gene            3609..4232
                     /locus_tag="AC16G0_15655"
     CDS             3609..4232
                     /locus_tag="AC16G0_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101090.1"
                     /translation="MNIQEWLTQLVSRPAAAPLDWESYRVTMDHATWKPLWRDINATQ
                     AYEDGLEAGLRLLEATQQHRGQLGPNGYRSNQVLLYRSILLMLDKAERWDAYLAAWET
                     IWAHTNECLPVRGDALLGGDPRLPPYVRRDDGGFGVPPLPYGVRPPKTIAVHFLYPQL
                     RRKTLIEKRLAHARAGKLASDRRPVGGGALTSEEIQSRLAQIRESAG"
     gene            4334..4789
                     /locus_tag="AC16G0_15660"
                     /pseudo
     CDS             4334..4789
                     /locus_tag="AC16G0_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505542.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GH36-type glycosyl hydrolase domain-containing
                     protein"
     gene            5078..5290
                     /locus_tag="AC16G0_15665"
     CDS             5078..5290
                     /locus_tag="AC16G0_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR5101091.1"
                     /translation="MRSVGTVKWFNDNKGFGFITLDGDQKDCFVHHSAIQVEGFKSLA
                     EGDRVEFDVVQGQKGPAAENVKKIAK"
     gene            5426..>5723
                     /locus_tag="AC16G0_15670"
     CDS             5426..>5723
                     /locus_tag="AC16G0_15670"
                     /inference="COORDINATES: protein motif:HMM:NF012305.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR5101092.1"
                     /translation="MSVRLFVGNLAYDVTEAELKELLSAAGTPLSVRVLTDRETGKPR
                     GFAFVEFADAAQAEEAVRRFHQQVFKGRPLVVNEARPREEGAGPRSSASGPFGRP"
BASE COUNT         1031 a         1703 c         2001 g          988 t
ORIGIN      
        1 aaggcccggg gcatgaatcc cctggggggt cacgacgagc gcgagcagac actcaaccag
       61 cttctcgtgg aaatggacgg gttcgaaacg aacaagggcg tcatcatcat ggcggccacg
      121 aaccgcccgg agatccttga tccggccctc ctccggcccg gccggttcga ccgccaggtc
      181 ctggtggacc ggccggacct caaggggcgc gaggccatcc tccgggtcca cgccaaaggg
      241 gtgacgatgg ccccgcaggt cgagctggcg gccgtcgcgg ccaagacccc tggattcgtg
      301 ggtgcggacc tggcgaacat cgtgaacgag gccgccctcc tggccgcccg ccaggggaag
      361 gatgcagtcg ggatggccga cttcgaggag gccatcgacc gggtggtcgc cggtctgaag
      421 aagacccggg tgatgagcgt caaggagcgg gagatcgtgg ccacccacga ggccgggcac
      481 gcggtggcgg cggcgtccct gccgggcacc gacccggtgg cgaaagtctc gatcatctcg
      541 cgggggatcg cggcgctcgg ctatacgcaa caactcccga cggaggaccg gtacctgatg
      601 acccggtcgg agctgtgcaa tcggctgtgc gtgctcctgg ggggccggac cgccgaggag
      661 atcgtctttg gcgatgtctc gaccggcgcc cagaacgacc tccagcgggc caccgatctc
      721 gtgcggcaca tggtggccca gtatgggatg agcgagaagg tcggcctgat gaccttcgac
      781 ggggccggac ggtcgggcct cctgccgggc ctggccccgc cgcgcgagta cagtgaagag
      841 acggcgcggc tcatcgacgc cgaggcgagc cgcctcctga tcgaggcgca cgtgaaggtc
      901 agcgccctcc tcacggagaa gcgaagtctt gtcgagcagc tcgcgcgccg gctgctggag
      961 aaagaggtgg tcgagcgaga cgagttgcag gccctgctcg ccggtgcgac gctctcctga
     1021 gtgggcgacc gggagcagcg gacgcgatgc aggcaggaag agcaatgagg gaagaggatg
     1081 atcatggacg aggagagatc gagctatccg taccttcgct acaccatcac gacgctccga
     1141 gctgagggcg tctggtgggc cagggcccgc gtggcggaga aggatgctgg gggcgatcga
     1201 ccggtcctgg gcggcccctg ggagagcaag ccggaggcga acaccgctgc acgcgcattc
     1261 tgcgacagcg ggaaggcggg gtgatgcaag ggtcctcgtg gcgccagtgg tccggggggc
     1321 ctggcccagg cgacgtggcg gcctcgcgga tgcccctgac cctgatccga gagatcggtt
     1381 gaatacttcg gattggcgct gagcgatcgt cggagacttt ccctccctga gagtggcagg
     1441 ggattcccgg tgggctgtcg ggggcagcat gccgcatccc gtcgagagac gcgatttgcc
     1501 cgaaggacac tttgagccaa cggggtcgag gatggccatg gtcgtttcca ccctgcgaat
     1561 cgtcacggtg ccccagtccc gggccgaggt ggtccggacg ctcgccgccc agctcgggcc
     1621 gacgcgggtg caaccgggct gcctccggtg tgatctctac cgagatgttg aagaccgggg
     1681 ggccatcacc ctggtggagg agtgggaatc gcaagccgat ctggacctgc gcttgcgctc
     1741 cgaggactac cgctccgtcc tggcggcgat cgagctggcg cgagagccgc ccgtgattca
     1801 cttcgacacg gtcaccagac gcgcaggctt ggagatcgtg gcgtcagcca gagggtagcc
     1861 tcgcctgccg cacctcctgc ctcgccatgg gatgcggcag tgtgagacga acaagaggaa
     1921 agagtgggac cgcgttaaaa ggaggacccg cgtgaacttt caggcgcgcc gtgcggatgg
     1981 aagcagcgat cgattcgccc caaccacaac actccagcct gccaagtcca tgtggaagcg
     2041 gatcgtagcc cgatatgaga agcctgccct gagtcgcagc gcgtggcagg cggcgaacac
     2101 gatcgtctcc tatggggtgc tgtggtgtct gatgtaccgg agcgtggcaa tttcctactg
     2161 gatcacgctc gccctggcga tccccacggc ggggttcttt gtgcggcttt tcatcatctt
     2221 ccacgactgc ggccacggct cgtttttcca gtcccggaag gccaatgagg cactggggat
     2281 tttcaccggg attctggtct ttaccccgta ctatcagtgg cggcacaagc atgccatcca
     2341 ccatgcgacc tcgggtgacc tggatcggcg tggcacgggc gatgtctgga ccatgacggt
     2401 gcgagaatac ctcgaggcct ctcgctggcg acggtttgcc tatcggctgg tacgcaaccc
     2461 ggcggtgctg ctcggcatta tcccgctcct cttgcttgtg gttgagcatc gattcccgtg
     2521 gcggaaggca gacaggcgag agcgcagcaa cgtctggtgg acgaatctgg ccattctcgg
     2581 gatggccatc ctgctgagcc ggctgatggg tcttcaggcg tacctggcga tccaggcgac
     2641 agtcctgatg atcgcagggt cggtcggcgt gtggttgttc tacgtccagc atcagttcga
     2701 cggcgcctac tgggagcacc gtgaccgctg ggattacgtg atcgcggcgc tccacggcag
     2761 ctccttctac aagttgccca gggtgctgca gtggttcacg ggcaacatcg ggttccacca
     2821 tattcaccat ctgagtcccg ggattccgaa ctataacctg gagaagtgtt accgggccga
     2881 tccgctgttt cagagggtcg cgcgggtaac attgctctcc agcctgaaag ccctcaccta
     2941 ccgcctgtgg gatgaggagc gacgcgtgtg ggtcggctac cggcacctga aggccctgcg
     3001 cgacggacga acgagagact gaggtgtgtt cgggcggggg ggaagcggcc gcgcgatgcg
     3061 ggagacagtc aggacaaccg cttgtgttga cacactgcct cgcagccgcg gagcgaaagg
     3121 ggttgaggat catgggaacc agactgtatg tgggaaatct tccgtttgac gtggatgaat
     3181 cgcagctccg gccgctcttc caggagggcg gacgaggggt caccgaggtc aagatggtcg
     3241 tggaccggga gacggggcgc ccccggggct tcgccttcgt cgagatggcg agccaggcgg
     3301 atgcccaggc ggccatcagc gcgctgaacg gccgggaact cgggggtcga gccttgaccg
     3361 tgaacgaggc ccgagagcgg gtgccacgca gcggtgggtt cgggggcggg cgttccagcc
     3421 actaaacacg caggataggc gtttgccgtc ccctgcgctc cgcaagattt ctgaatctgt
     3481 tgacaccttg tatcctgatt ttctcttgcg gagcaccagg atgggtcacc ggatcgaatt
     3541 gttgcggagc acaccgtccg gcatttctcc ggctccccgt gccattcggc aaaatgcatg
     3601 aggactccat gaacattcag gaatggctta cgcagctcgt gtcgcgcccg gcagcggctc
     3661 cacttgactg ggagagctac cgcgtcacca tggaccatgc gacgtggaag cccctctggc
     3721 gggacatcaa tgcaacgcaa gcctacgagg atggcctgga ggcgggcttg cgcctcctcg
     3781 aggccacgca gcaacaccgc gggcagctcg gtccgaatgg gtaccgctcg aaccaggtcc
     3841 tgctctaccg cagcatcctc ttgatgctcg acaaggccga acgctgggat gcgtatctgg
     3901 ccgcctggga gacgatctgg gcgcacacga acgaatgtct tcccgtcagg ggtgatgcct
     3961 tgctgggtgg cgatccccga ctcccgccct atgtccggcg agacgatggt gggttcggcg
     4021 tcccgccgct cccgtatggc gtgcggcccc cgaagaccat cgccgtgcat ttcctctacc
     4081 ctcaactccg acgcaagacg ctcatcgaga agcgcctggc ccacgcgcgg gccgggaaac
     4141 tggcctcgga tcgccggccc gtgggcgggg gcgccctgac gtccgaggag attcagtcgc
     4201 ggctcgccca gatacgggag tcggccgggt agatgaggac gtgcctcggt ccgccccccg
     4261 gctccgtccc tgacgaccca cgcaacagaa tgaaccgccg acgatcacgt caagcgagag
     4321 tctcctggca cggcgggcgt gggaactctt gagaatggtc aacccggtga accatgggag
     4381 gtctccggag gcagtggcaa cctacaaagt cgagccgtac gtcgtcgcgg ccgacgtgta
     4441 tgcgctctcg ccgcacatcg gccgtggcgg atggacgtgg tacacgggct cggccggctg
     4501 gatgtaccgg ttgatcctgg aatcactttt ggggctgagg ctggatatag acaggctgcg
     4561 ggttgccccg tgcctcccgg cggattggac cgggttcacc gtgcactacc ggtatcggga
     4621 aactgtgtac cacatcgccg tcctgcaaac gcgcgccgga tatggcgagg tgggtgtgac
     4681 agttgatggg gttgagcagg acgacaaggc aattcgacca gttgacgacc gtcgggaaca
     4741 cgcggtcgag gtcagagtat ccgcgtcagg cgctaagtgc agcgtgtagg gtgatcgctc
     4801 ctagtcgtcg ccaggacgcc tgaggaccgg ctgtggcggc ggccaggtgc ttccggcgcc
     4861 cacgtcaaag ctgggcgatc ttcccacggg tgcgattcct tggccctggg cagaacaatc
     4921 gtgggaaatc aattgacaga gtcggcgggg tctgggatag tgtgtcatct caaatcgcca
     4981 tgtcggtgtg ttattgaatc aggggccagg aaggacccga caagaggagg gtcctttttt
     5041 tatttcctga aataccctcg aggaagggag tgtcacgatg cgcagcgttg gaaccgtgaa
     5101 gtggttcaat gacaacaagg gcttcggctt catcacgctg gacggtgacc agaaggattg
     5161 cttcgttcac cactcggcta ttcaggtcga ggggttcaag tcgctggcgg aaggggaccg
     5221 cgtggagttt gacgtcgtgc agggacagaa ggggccggcg gcggagaacg tcaagaagat
     5281 cgccaaatag aggcgaaaaa ctcaagcagg cgcaccgcac agacacagaa cgttttgggt
     5341 ctgctagcga cccacctggc gcactccggg atgactggga tgcggagtgc ccgtgcgtgc
     5401 gtacggggca accgagggag gatcgatgtc ggttcggctc ttcgtgggga atctggcgta
     5461 cgatgtgacg gaagccgagt taaaggagct tctctccgca gccgggacac cactgtccgt
     5521 gcgggttctc accgaccggg agactggcaa gccgaggggg tttgccttcg tcgagttcgc
     5581 cgatgccgcc caggcggaag aggccgtccg ccgttttcac cagcaggtgt tcaagggccg
     5641 gccgctcgtg gtgaacgagg cgcgcccccg ggaggagggg gccggcccaa ggtcgagcgc
     5701 ttccggcccg ttcggccggc cgg
//
LOCUS       DBNGZC010000905         1626 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1738455, whole genome shotgun sequence.
ACCESSION   DBNGZC010000905 DBNGZC010000000
VERSION     DBNGZC010000905.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1738455"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="AC16G0_15675"
     CDS             <1..773
                     /locus_tag="AC16G0_15675"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5101093.1"
                     /translation="RRVAMKLRGKVAIVTGGGRGIGRAIALALGREGAAVALAARSAP
                     EIEAVAREVRDLGAQALPVDTDVAREEDIARLSRRTLEAFGRIDILVNNAGIGLPLRD
                     VVDLGLDEWSRVLQVNLTGPFLCAKAVLPSMIRQRWGKIINVSSLGGQVGVAGNSAYG
                     ASKAGLLLFTRCLAAEVKRYGIDVNAICPSGTDTQLLREMGRAEGRTNLMRPEEIANT
                     ILFLVSPESSAVTGTVIEAYGLSNPLFGPLTPGEQEES"
     gene            1038..>1626
                     /locus_tag="AC16G0_15680"
     CDS             1038..>1626
                     /locus_tag="AC16G0_15680"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HIR5101094.1"
                     /translation="MISPFPSGLAPGAARRQAGALLVLFYGLVYGGAILFGGRHDRLA
                     APILSALVLGLLMLLAIGLCVRRDASWRESLGLGPQPVGTVLGWSLLGFAGTYAVNLA
                     LISLYLSLQGNLEAIAAHRATWLGKLAEVPTEAILPLAAFAAVWEETVFRGFLLGRLR
                     AAMPALNTRGAALRRDLLAVVLTALFFGAGHGYQGG"
BASE COUNT          241 a          532 c          559 g          294 t
ORIGIN      
        1 ctcggagggt ggccatgaag ctccgcggca aagtggcgat cgtcacgggg ggcggcaggg
       61 ggatcggacg cgcaatcgcc ttggcactgg gcagggaggg ggcggcggtt gccctggcgg
      121 cgcgcagtgc cccggagatc gaggccgtgg cccgcgaggt gcgggacctg ggcgcccagg
      181 ccctacctgt tgacaccgac gtcgcgcgtg aagaagatat cgcccgcctg agccgacgga
      241 cgctggaggc cttcgggcgg atcgacattc ttgtaaacaa tgccggcatc ggcctcccgt
      301 tgcgggacgt ggtggatctc ggcctggacg agtggagccg cgtcctgcag gtcaacctga
      361 cgggcccgtt tctgtgcgcg aaggccgtcc tcccgagcat gatccgccag cgctggggca
      421 agatcatcaa cgtctcttct ctgggcggcc aagtcggggt cgcgggcaac agcgcctatg
      481 gggcgtccaa ggcaggcctt ctccttttca cgcgctgcct ggcggccgag gtcaagcggt
      541 acggaatcga cgtcaacgcg atctgtccca gcggcacgga cacccaactg ctccgcgaga
      601 tggggcgggc cgagggccgc accaacctga tgcgacccga agagatcgcc aacaccatcc
      661 tgttcctggt ctcgccggag tcctcggccg tcacaggcac cgtgatcgag gcctatggcc
      721 tgagcaaccc cctcttcggt cctctcaccc cgggtgaaca ggaggagtcg tagggctcca
      781 gctagtgacc aatcccagag attcgcagca taaaccggag cccccatggg taccccgaaa
      841 gccccggccc gcggctcggc cgggttcgtg atggtgtgtc tgcatggggg tagaaacctg
      901 ctgaacatct ctgggtaggt cgtctatgtc gaagaggcag gtgctgttga ttttgatcac
      961 tggctctggg cgctactcga ctcccaggcc cgcccgccgg gatgacttgc cttggctaat
     1021 ccggctataa tgaaggcatg atctccccct tcccatcagg actcgcgccc ggcgcggcgc
     1081 ggcggcaggc cggggcgctg ctggtcttgt tctacggcct ggtctacggt ggcgcgattc
     1141 tctttggcgg caggcacgac cggctggcgg cgccgatact ttcggcgctt gtcctcgggt
     1201 tgctcatgct cctcgccatc ggactctgcg ttcgccgcga tgcgtcatgg cgtgaatcgc
     1261 tcgggctcgg cccacagccg gttgggacgg tgctcggctg gagcctgctc ggctttgccg
     1321 gcacgtacgc cgtgaacctg gcactcatca gcctgtatct cagcctccaa ggcaacctcg
     1381 aggccatcgc ggcgcaccgg gcgacttggc tcgggaagct ggctgaggta ccgacggagg
     1441 cgatccttcc gctcgccgcc tttgccgccg tctgggaaga gactgtcttc cgcggcttcc
     1501 tcctcggccg tctgcgtgcg gcgatgccgg cgctgaatac caggggggcg gcgctgcgcc
     1561 gtgacctgct ggccgtcgtg ctcacggcgc tcttcttcgg cgcgggccac ggataccagg
     1621 ggggac
//
LOCUS       DBNGZC010000906         2377 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2330263, whole genome shotgun sequence.
ACCESSION   DBNGZC010000906 DBNGZC010000000
VERSION     DBNGZC010000906.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2330263"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..395)
                     /locus_tag="AC16G0_15685"
     CDS             complement(3..395)
                     /locus_tag="AC16G0_15685"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR5101095.1"
                     /translation="MDTNVFLRLLTRDDPVKFNRCRRLFEQAVAGSITLHTSELVIAE
                     IVWTLLSYYDLAKPTVIEKVGQILNTPNLIVKNQEALIEGLALWSRHDCDFIDAYNAA
                     VMHRDGLPNLASYDSDFDSFPLIRRQEA"
     gene            complement(410..670)
                     /locus_tag="AC16G0_15690"
     CDS             complement(410..670)
                     /locus_tag="AC16G0_15690"
                     /inference="COORDINATES: protein motif:HMM:NF015947.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR5101096.1"
                     /translation="MPIAAITSKGQMTLPKTIRDSLGLKPTDRVVLVMEGDHAILYPL
                     RGTILDAAGSVKPRSGKRPLDFRAVRGEVRRRLAYRAVSRGR"
     gene            776..1540
                     /locus_tag="AC16G0_15695"
     CDS             776..1540
                     /locus_tag="AC16G0_15695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR5101097.1"
                     /translation="MPRGDLQVRLERARSVAVLTGAGVSAESGVPTFRGSDGLWRRYR
                     AEDLAAPEAFRRDPVLVWEWYDWRRQLIAKCRPNPAHHAIADLEGRFQDFLLITQNVD
                     GLHQKAGSRRMAELHGSLWRVRCLEDAEITEQHEVPLRAVPPRCTCGGLLRPDVVWFG
                     EALPPDAIREAYQAAESCDVMLVVGTSALVQPAASLPLVSKQHGAYVVEVNLEPTTLT
                     EMADESHQGKAGEILPRLLGCDSATRNARREIPEGP"
     gene            1543..>2377
                     /locus_tag="AC16G0_15700"
     CDS             1543..>2377
                     /locus_tag="AC16G0_15700"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HIR5101098.1"
                     /translation="MFSLKLAPGKDTRLLGGHPWIYRSEIARVDGEGEPGAIAHVLDN
                     RGKFLGQAMVNLKSQIAGRLLSRKEEPIDAAFLERRLREAVARCDRSVSGPDACRLVF
                     GEGDHLPGLIADRYGGLVVLQILTAGMERLREPLLEALRSVVGPQAIFERNDAPPRKL
                     EGLELRKGFAWGGGETGVWIQEGGLRYFVEVAEGQKTGFFLDQRENRRVVRSLARDRE
                     VLDCFCYSGGFALSAAAGGAASVTGIDLSAEALAWAQRSAEQNGLASRCTFIAANAFD
                     EL"
BASE COUNT          424 a          677 c          850 g          426 t
ORIGIN      
        1 aatcaggcct cctggcgtct gatgagtggg aaggaatcga agtccgaatc gtaggacgcg
       61 aggtttggca ggccgtcacg gtgcatcacg gcggcgttgt aggcatcaat gaagtcgcag
      121 tcgtgacggc tccagagagc cagcccctcg atcaaagcct cctggttctt gacgatcagg
      181 ttcggcgtgt tgaggatctg gccgaccttc tcgattacgg tgggcttagc caggtcgtaa
      241 tacgacagta gtgtccaaac gatttcggcg ataacgagtt ccgaagtgtg cagggtaatc
      301 gagccggcca cggcctgctc gaaaagacga cgacatcgat tgaatttgac agggtcgtcc
      361 cgggtcagca atcgcagaaa gacattggtg tccacgaaca gggaaggcat cagcggcccc
      421 gggatacagc ccgatatgcg agtcgccgcc ggacctcacc acggaccgcg cgaaagtcca
      481 acggcctttt tccggaccgg ggtttgaccg atccggcggc atccagaatc gttccgcgga
      541 gtgggtagag gatggcgtga tcgccttcca taaccaggac gactcggtct gtgggcttga
      601 ggcccaatga gtctcggatg gtcttgggaa gggtcatctg ccccttggat gtgatcgcgg
      661 caatcggcat ggcagaatcc tcctcttatt gatgcagtat ggctgaagta aggaaaatat
      721 gtcaactcat tactttttgg attacgaagg gaacccgatt ggactgtgaa ggatgatgcc
      781 caggggggac cttcaggtac gtctcgagcg ggcgcggtcc gtggctgtgc tgaccggcgc
      841 cggggtgtcg gcggaaagcg gcgttccgac gttccgcggg tcggacgggc tgtggcgccg
      901 gtatcgggcc gaggatctgg cagctccgga ggccttccgg cgggatccgg tgctggtctg
      961 ggaatggtac gactggcggc gccagctcat cgcgaagtgc cggccgaacc cggcccacca
     1021 tgccatcgcc gacctggaag gccgatttca agacttcttg ctgatcacgc agaacgtgga
     1081 cgggctgcac cagaaggcgg gtagcagacg aatggcggag ttgcacggga gtctctggcg
     1141 cgtccgctgc ctggaagacg cagagatcac ggagcagcac gaggttcccc tgcgggcggt
     1201 tccgccgcgc tgcacctgtg gggggctcct gcggccggac gtggtctggt tcggcgaggc
     1261 gctgccgccg gacgccatcc gagaggccta tcaggctgcg gagtcctgcg acgtcatgct
     1321 ggtcgtcggg acctcggccc tggtccagcc ggcggcttcg ctgccgctgg tgagcaagca
     1381 gcacggcgcc tacgtggtcg aggtgaacct ggagccaacg acgctgaccg agatggccga
     1441 cgagtctcac caggggaagg ccggggagat ccttccgcga cttctcggct gtgattccgc
     1501 aacccgcaat gcgagaaggg aaatcccgga ggggccttga cggtgttcag cctgaaactt
     1561 gctcccggca aggatacgcg gttgctcggg gggcatccct ggatataccg gagcgagatc
     1621 gcccgcgtgg acggcgaggg ggagccagga gccatcgccc atgtcctgga caaccggggg
     1681 aagttcctcg gccaggcgat ggtgaacctg aagtcgcaga tcgccggccg tctgctctcc
     1741 cggaaagaag aacccatcga cgcggcgttt ctggagcgcc gtctgcgtga ggcggtggcg
     1801 cgatgcgacc gcagcgtgag tgggccggac gcctgtcgtc tcgtcttcgg cgagggggat
     1861 caccttccgg gcctcatcgc ggaccggtac ggggggctgg tggtgctcca gatcctcacg
     1921 gcgggaatgg agcgactgcg cgagccgctg ttggaggcct tgcgcagcgt ggtagggccc
     1981 caggccatct tcgaacgaaa cgacgcccct ccgcgaaagc tggagggcct ggagcttcgc
     2041 aaaggcttcg cctggggtgg aggggagacc ggggtctgga tccaggaggg tgggctgcgg
     2101 tatttcgtgg aggtggccga agggcagaag accggcttct tcctcgacca gcgcgagaac
     2161 cgccgggtgg tcaggagcct cgccagggat cgcgaggtgc tggattgttt ctgctactcc
     2221 ggcggatttg ccctgagcgc ggcggccggc ggtgccgcct cggtgaccgg gatcgaccta
     2281 tcggccgagg ctctggcgtg ggcacagcgg agcgcggagc agaatggtct tgcctcccga
     2341 tgtacgttca tcgcggccaa cgcctttgac gagctcc
//
LOCUS       DBNGZC010000907         4675 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1447816, whole genome shotgun sequence.
ACCESSION   DBNGZC010000907 DBNGZC010000000
VERSION     DBNGZC010000907.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1447816"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3260
                     /gene="gltB"
                     /locus_tag="AC16G0_15705"
     CDS             <1..3260
                     /gene="gltB"
                     /locus_tag="AC16G0_15705"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HIR5101099.1"
                     /translation="TEKPYAEWLRQHSVTIQELPEAPVHEPDHETVLRRQQVFGYTHE
                     DLRILLAPMAVNGQEPVGSMGTDTSLAVLSNRPRMLYDYFKQLFAQVTNPPLDAIREE
                     LVTSMESTIGPERNLLNAEPESCHQIAIPVPILNNHEVAKLRHITTHGFRSVTLPMLF
                     PVEEDGPGMAAALEELCQKASQAIERGYNILILSDRGVDLEHAPIPALLATAAVHHHM
                     IREGTRTKVGMVVETGEAREVHHCALLMGYGAGAINPYLAFETLDDMIQQGLLPEGMT
                     HEKAVKNYIKALNKGILKVISKMGISTIQSYCGAQIFEAIGLNQDFVDRYFTWTASRI
                     GGVGLEVVAEETMRRHGQAFPARLVEKPDLDWGGEYQWRREGEYHLFNPDTVFKLQHA
                     TRSGQYQVFKEYSELVNNQSQRLCTLRGLLELKLAEKPIPLEEVEPVESIVKRFATGA
                     MSYGSISQEAHETLAIAMNRLGGRSNTGEGGEDPTRFARDPNGDWRRSAVKQVASGRF
                     GVTSEYLVNAEELQIKMAQGAKPGEGGQLPGHKVYPWIAKVRYSTPGVQLISPPPHHD
                     IYSIEDLAQLIYDLKNANPTARVSVKLVAEVGVGTVAAGVSKAHADVVLISGHDGGTG
                     ASPLTSIKHSGIPWELGLAETQQVLSLNDLRGRIVVQTDGQLKTGRDVVVAALLGAEE
                     FGFATAPLVVMGCVMMRVCHLNTCPVGVATQDPELRKRFTGQPEHVENFFRFIAQEVR
                     ELMAQLGFRSMDEMIGRSDKLDMRKAVDHWKARGLDFSKILYRPDLPPHVAVRRVQSQ
                     DHGLEKALDNRLVALAKPALEQGIPVELSLPIRNTNRTVGTILGYHVTKRYGGRGLPD
                     ETIRIKFTGSAGQSFGAFLPRGITLTLEGDANDYLGKGLSGGRIIAYPPAGSTFVPEE
                     NILVGNVVLYGATSGEAFFRGMAGERFAVRNSGAYTVVEGVGDHGCEYMTGGRVVVLG
                     KSGRNFAAGMSGGIAYVLDEAGDFERQCNKEMVDLEALGEPEDVTLVWDLLRKHAECT
                     QSARAKKILANWERMLPKFVKVMPRDYRRVLTAQAKARAEGREPQWEELLGVSHG"
     gene            3253..>4675
                     /locus_tag="AC16G0_15710"
     CDS             3253..>4675
                     /locus_tag="AC16G0_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020731107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HIR5101100.1"
                     /translation="MGKPTGFLEITRELPKRRPVEERVKDWQEIYLDFPKEKLRAQGA
                     RCMDCGIPFCHQGCPLGNVIPDWNDLVYRNRWREAIDRLHATNNFPEFTGRLCPAPCE
                     GSCVLGINQPAVTIRQIELEIIEHAFHEGWIKAEPPLDRTGKKVAVIGSGPSGLAAAQ
                     QLNRAGHWVTVFERADRIGGLLRYGIPEFKMEKRILDRRVKLLVEEGIAFVTNANVGV
                     DVPVEALRQTYDAILLAGGATAPRDLKIPGRELEGIHFAMEYLPLQNRRCQGDEIPEE
                     HFISAHGKRVVIIGGGDTGADCLGTTHRQGARSVHQFELLSEPPKERSHENPWPLWPN
                     IFRTSSAHEEGGVREYCISTTRFTGRDGRVERLHAVRVEFVSNGTSRPTMREVPGSAF
                     ELEADLVLLAMGFLGPEKDGLLAQLGVAMTDRGTVAVDADKMTSVSGIFSAGDMARGQ
                     SLIVWAIAEGRQAAKGVDRYLMGS"
BASE COUNT          843 a         1471 c         1625 g          736 t
ORIGIN      
        1 ctacggagaa gccgtacgcc gagtggcttc gccagcactc ggtgaccatc caggagttgc
       61 ccgaggcacc cgtccacgag ccggatcacg agaccgtcct gcgccgccag caggtcttcg
      121 gctacaccca cgaagatctg cggatcctcc tggcccccat ggctgtgaac gggcaagagc
      181 ccgtggggtc catggggacg gacacctcgc tggccgttct ctcgaaccgg ccgcggatgc
      241 tctacgatta cttcaagcag ctattcgccc aggtcacgaa cccgcccctg gacgccatcc
      301 gcgaggagct ggtgacgtcc atggagagca ccatcggccc ggagcggaat ctcttgaacg
      361 ccgagcccga gagctgccat cagatcgcca tcccggtgcc catcctgaac aaccacgagg
      421 tggccaagct gcgccacatc accacccacg gcttccgctc ggtgacgctc ccgatgctct
      481 tcccggtgga ggaggatgga ccgggcatgg cggcggccct ggaggagctg tgtcagaagg
      541 caagccaggc catcgagcgc ggctacaaca tcctgatcct gtccgaccgc ggcgtggatc
      601 tggagcacgc ccccatcccg gccctcctgg cgacggccgc cgtccaccac cacatgatcc
      661 gcgaggggac ccgcaccaag gtcgggatgg tggtcgagac cggcgaggcg cgtgaggtcc
      721 atcactgcgc cctgctcatg ggctacgggg cgggggccat caatccctac ctggccttcg
      781 agacgctgga cgacatgatc cagcagggac tccttccaga ggggatgacc catgagaagg
      841 ccgtcaagaa ctacatcaag gccttgaaca agggcatcct caaggtcatc tccaagatgg
      901 ggatctccac gatccagagc tattgcgggg cccagatctt cgaggccatc ggcctgaacc
      961 aggatttcgt ggaccggtac ttcacctgga cggcgtcgcg gatcgggggc gtgggcctgg
     1021 aggtggtcgc cgaggaaacc atgcggcggc atgggcaggc gttccccgcc cggctcgtgg
     1081 agaagccgga cctggactgg ggcggcgagt accagtggcg gcgggagggg gagtaccacc
     1141 tcttcaaccc cgacacggtg ttcaagctgc agcacgccac gcggagcggc cagtaccagg
     1201 tcttcaagga gtactcagag ctggtcaaca accagagcca gcggctgtgc accctgcggg
     1261 gcctgctgga gctcaagctc gccgagaagc ccatccccct ggaagaggtc gagccggttg
     1321 agagcatcgt caagcgcttc gccacggggg ccatgtccta cgggtccatc agccaggaag
     1381 cccacgagac cctggccatc gccatgaacc ggctgggcgg acggagcaac accggcgagg
     1441 ggggcgagga cccgacgcgg ttcgcgcgcg acccgaacgg ggattggcgt cggagcgccg
     1501 tgaagcaggt cgcctccggg cgattcgggg tgaccagcga gtacctggtg aacgccgagg
     1561 agcttcagat caagatggcc cagggcgcca agcccggcga gggcggccag cttcccggcc
     1621 acaaggtcta tccctggatc gccaaggtcc gctattccac gccgggggtg cagttgatct
     1681 cgcccccgcc gcatcacgac atctactcga tcgaggacct ggcccagctc atctacgacc
     1741 tgaagaacgc gaatcccacc gcgcgggtga gcgtgaagct cgtggccgag gtgggcgtgg
     1801 ggacggtggc cgccggggtg agcaaggccc acgcggacgt ggtcctgatc agcgggcacg
     1861 atggggggac cggggcctcg ccgctcacca gcatcaagca ctcgggcatc ccgtgggagc
     1921 tgggtctcgc cgagacgcag caggtcctca gcctgaacga cctgcgaggt cgcatcgtcg
     1981 tccagaccga cgggcagctc aagaccgggc gggatgtggt ggtggccgcc ctcttggggg
     2041 cggaggagtt cgggttcgcc acggccccgc tggtggttat gggctgcgtc atgatgcggg
     2101 tctgccacct gaacacctgt ccggtcggcg tggccacgca ggaccccgag ctccgcaagc
     2161 ggttcacggg tcagccggag cacgtcgaaa acttcttccg tttcatcgcg caagaggtgc
     2221 gagagctgat ggcgcagctc ggcttccgca gcatggacga gatgatcggg cgctctgaca
     2281 agctggatat gcggaaagcg gttgaccact ggaaggcgcg cgggctggac ttctctaaga
     2341 tcctctaccg tccagacctg ccgccacacg tcgccgtgcg tcgcgttcag tcgcaggacc
     2401 acggcctgga gaaggccctg gacaaccggt tggtcgcgct ggcgaagccc gctctggagc
     2461 aggggatccc cgtcgagctg tccctcccca tccggaacac caaccggacc gtggggacga
     2521 tcctgggata tcacgtgacc aagcgttacg gagggagggg actgccggac gagacgatcc
     2581 gcatcaagtt taccggctcg gccgggcaga gcttcggcgc cttcctcccg cgggggatca
     2641 cgctgacgct ggagggggat gccaacgact acctggggaa aggcctctcc ggcgggcgga
     2701 tcatcgccta tcctccggcg ggctcgacct tcgtgccgga agagaatatc ctcgtcggaa
     2761 atgtcgtgct ctacggcgcc acctccgggg aggcgttctt ccgtggcatg gccggggagc
     2821 ggttcgccgt caggaatagc ggcgcctaca cggtggtcga gggggtgggc gaccacggtt
     2881 gcgagtacat gaccggtggc cgggtggtgg tcttggggaa gagcggccgg aacttcgccg
     2941 ccgggatgag cgggggcatc gcctacgtcc tggatgaggc gggggatttc gagcggcagt
     3001 gcaacaagga aatggtggac ctggaggctt tgggcgagcc ggaggatgtc acgctggtct
     3061 gggacctcct ccggaagcat gcggagtgca cgcagagcgc ccgggccaag aagatcttgg
     3121 cgaactggga acgcatgctg ccgaaattcg tgaaagtcat gccgcgggat taccgccggg
     3181 tcttgacggc ccaggccaag gcccgcgccg aaggccgaga gccccagtgg gaggagctgt
     3241 tgggggtgag ccatgggtaa gcccaccgga tttctggaga tcacccgcga gctgccgaag
     3301 cggcgccccg tcgaagagcg ggtgaaggac tggcaggaga tctacctgga cttccccaag
     3361 gagaagctgc gggcccaggg tgcgcgctgc atggactgcg ggatcccctt ttgccatcag
     3421 ggctgccccc tcggaaatgt catccccgac tggaacgacc tggtctatcg gaaccgctgg
     3481 cgggaggcca tcgatcgtct gcatgccacg aataactttc cggagttcac cgggcggctc
     3541 tgtcccgcgc cctgcgaggg ctcgtgcgtc ctggggatca accagccggc ggtgacgatc
     3601 cggcagatcg agctcgagat catcgagcac gctttccacg agggctggat caaggccgag
     3661 ccgcccctgg atcgcaccgg gaagaaggtt gccgtcatcg ggtccgggcc aagcggcctg
     3721 gccgccgccc agcagctcaa tcgggccggt cactgggtga ccgtcttcga gcgggcggat
     3781 cggatcggag gtctcctgcg ctacgggatc ccggaattca agatggagaa gcgcatcctg
     3841 gatcgccggg tcaagcttct cgtcgaggaa ggtatcgcct ttgtgacgaa cgccaatgtg
     3901 ggtgtcgacg tgccggtcga ggccctgcgc cagacctatg acgcaatcct gctggccggc
     3961 ggcgccacgg cgccccgcga cctcaagatc cccggccgcg agttggaggg gatccatttt
     4021 gccatggagt acctgccgct gcaaaaccgg cggtgccagg gtgacgagat tccagaggag
     4081 cacttcatct cggcgcacgg aaagcgggtg gtgatcatcg gtgggggcga caccggggcc
     4141 gactgcttgg gaacgacgca ccggcagggt gcgcgctcgg tgcaccagtt cgagttgctt
     4201 tccgagcccc ccaaggaacg gtcgcacgag aacccgtggc cgctctggcc caacatcttc
     4261 cggacgtcct ccgcccacga ggaaggcggc gttcgggaat attgcatttc cacgacgcgg
     4321 ttcacggggc gggacggccg ggttgagcgt ctccacgcag tccgcgtgga gttcgtgtcg
     4381 aacggcacca gccgaccgac catgcgcgag gtccccggga gcgcgttcga gctagaggcg
     4441 gatctggtct tgctcgccat gggtttcctg ggaccggaga aagatggcct gctggcccag
     4501 ctcggggtag cgatgaccga tcggggtacc gtggctgtgg atgctgacaa gatgacgagc
     4561 gtctctggga tcttctcggc cggcgacatg gcccggggac agtccctcat cgtttgggcc
     4621 atcgccgaag gtcgccaggc tgccaaaggt gtcgacaggt acctcatggg atcaa
//
LOCUS       DBNGZC010000908         2342 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1148335, whole genome shotgun sequence.
ACCESSION   DBNGZC010000908 DBNGZC010000000
VERSION     DBNGZC010000908.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1148335"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="AC16G0_15715"
     CDS             complement(<1..532)
                     /locus_tag="AC16G0_15715"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5101101.1"
                     /translation="MRLPVIVKVSLFALLVMGVYTYVANSIPQIQSVPQQELSLEGGN
                     VTTEQLVKAGEGIFKGKGTCEICHAIGQKGTRAPDLAGVGARAGKRKPGMSAKAYLIE
                     SLLDPGAYLVEGYPNIMPKVDRPPIGLNRSELWALVTFLESQGGTPDVKLEDIPKSAG
                     FVAGGGAGPAAELKLPG"
     gene            complement(534..>2342)
                     /locus_tag="AC16G0_15720"
     CDS             complement(534..>2342)
                     /locus_tag="AC16G0_15720"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HIR5101102.1"
                     /translation="LVEELRLEEGQRPPGAGTWKWASILSGTILLLTAAAAWGQAGGG
                     EPSPAYRNFPLFGSRLVVWGAAQLHLDFAAFILGVPIFAVIVEFVGWRTREPRYDWLA
                     HEFMKLTFAAFSATALLGGALTFFLLTLYPRFFGYLAGIFGPTMWVYAGLFFLETFTL
                     YLYYYGWDWLGGSRKGIHLILGVLLNLWGTALLFVANSWVTFMVSPRGVDQAGKLTSL
                     WAAIDNSTWMPINIHRLIANVTFGGAIAAAYAAVRFLGATTDEERAQYDWMGYIGNFV
                     AISSLLFLPFAGYWLGREIYAFNQTMGITMMGGFLSWLWVIQALLIGVLFLGSNYYLW
                     LGMERIPGSERYRQFIPYMILILAAGFMVWATPHSMVVTLEESRAMGGTHHPVLGLLG
                     VMSAKNTAVNLMILTTYLSFLLYRRANKVSQKPWVKTGMAVQWALFGVAAAVVIFFGV
                     YGYFVESIVRIGFSVYQVLAVLSAIVLTILIDIPMFRGAQSTGSIRWGRIAPRSQYVL
                     ILLAVTFAWLMGLMGFARSGIRQDWHVYGVIRDTSPEAWTPALGYAAKMISLVTIVFF
                     ALVTLIFWLGGLGEKRRTPAGATEASPVAHGTTPGD"
BASE COUNT          509 a          738 c          711 g          384 t
ORIGIN      
        1 cgcccggaag cttcaattcc gccgccggcc cagccccgcc gcctgcgacg aaccctgcgc
       61 tcttggggat atcctccagc ttcacatccg gcgtcccacc ctgcgactcc aggaatgtga
      121 caagtgccca gagttcggag cggttcaagc cgatgggcgg ccggtccacc ttcggcatga
      181 tgttaggata gccttccacc aggtaagcgc cgggatccag cagggactcg atcaggtagg
      241 ccttggcgct catgccgggc tttcgttttc ccgcgcgggc gccgacgcct gccaagtcgg
      301 gcgcccgggt gcccttttga ccgatggcgt ggcagatctc acaggtaccc ttgcccttga
      361 agattccctc gccggccttc acgagctgtt ccgtggtgac gttgccgcct tccagggaca
      421 actcctgttg aggcacggac tgaatctggg ggatcgagtt ggcgacatac gtgtacacgc
      481 ccatcaccag cagcgcgaag agactgacct tcacgatcac cggcaatcgc atctcaatct
      541 cccggagtcg ttccatgcgc taccggcgac gcctcggtgg cgccggccgg cgttcttctc
      601 ttttcgccga gcccccccag ccagaagatc aaggtcacga gggcaaagaa aacaatggtt
      661 acgaggctga tcatcttggc tgcgtaaccc agggccggag tccacgcctc gggtgaggtg
      721 tcgcggatca cgccgtacac atgccagtcc tgcctgatgc ccgaccgggc aaatcccatg
      781 agccccatca gccaagcaaa tgtcaccgcc agcaggatca agacatactg tgaccggggg
      841 gcgatccgtc cccagcggat ggagcctgtc gattgggcgc cccggaacat gggaatgtca
      901 atcaggatgg tgagaacgat ggcactcaag acagccagaa cctggtacac cgagaatcca
      961 atacggacga tggactccac gaagtagccg tagacaccga aaaagatgac caccgcagcg
     1021 gccacaccaa agagtgccca ctgcacggcc ataccggtct tcacccacgg cttctgcgag
     1081 accttattcg cccgccggta caacagaaaa gagagatacg tcgtgaggat catcagattg
     1141 acagccgtgt tcttggccga catgactccc agcagaccca gcaccggatg atgggtgccg
     1201 cccatggccc gggattcctc cagggtcacc accatgctgt gcggcgtggc ccacaccatg
     1261 aatcccgctg ccaggatcag gatcatgtag ggaatgaact gccggtatcg ctctgacccg
     1321 gggatacgct ccatcccgag ccagaggtag tagttcgatc cgaggaagag gaccccgatg
     1381 agcaaggcct ggatcaccca gagccatgac aggaacccgc ccatcatcgt gatgcccatc
     1441 gtctggttga aggcgtagat ctcccgcccc agccagtagc cggcaaacgg caggaagagc
     1501 agcgacgaga tcgccacgaa gttgccgata tagcccatcc agtcatactg ggcgcgctcc
     1561 tcgtcggtgg tggctcccag gaagcggacg gcggcgtagg ctgcggcaat ggcaccgccg
     1621 aatgtcacgt tggcgatcag gcgatggata ttgatgggca tccaggtgct attgtcaatg
     1681 gcggcccaca ggctggtgag ctttcccgcc tggtccacgc cgcgaggcga gaccatgaag
     1741 gttacccagg agttcgccac gaagagcaga gccgtccccc acaggttcag gaggacaccg
     1801 agaataaggt gaatcccttt ccgggagccg cccagccaat cccaaccgta gtagtagagg
     1861 tagagcgtga acgtttccag gaagaagagt cctgcgtaca cccacatcgt cggcccgaag
     1921 atgccggcca ggtacccgaa gaagcgggga tacagggtga ggaggaaaaa ggtcagcgcg
     1981 ccccccagga gcgcggtggc ggagaaggcg gcaaaggtga gcttcatgaa ctcgtgggcc
     2041 agccagtcgt agcgtggctc ccgggtgcgc cagcccacga actccacgat gacggcgaag
     2101 atgggaacac cgaggatgaa ggcggcgaag tccagatgga gttgggcggc gccccagacc
     2161 accaagcgac tgccgaacaa cggaaagttc cgataggcgg gcgagggttc gccgcctcct
     2221 gcctggcccc aagccgcggc agccgtcagg aggagaatcg ttccggagag aatgcttgcc
     2281 cacttccagg tccctgcacc tgggggtctc tgcccttcct ctaaacgcaa ttcttctacg
     2341 ag
//
LOCUS       DBNGZC010000909         3639 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2132692, whole genome shotgun sequence.
ACCESSION   DBNGZC010000909 DBNGZC010000000
VERSION     DBNGZC010000909.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2132692"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="AC16G0_15725"
     CDS             complement(<1..300)
                     /locus_tag="AC16G0_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HIR5101103.1"
                     /translation="MPRADREPNIVPSILDRLLDDRPEVSAEAIPIRTQTVRQLERSV
                     ARDLEELLNTRREMLEELPPDFVEVSRSLVNYGPPDFTAFSLLGEKDRARIRRILE"
     gene            complement(300..1058)
                     /locus_tag="AC16G0_15730"
     CDS             complement(300..1058)
                     /locus_tag="AC16G0_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015250408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HIR5101104.1"
                     /translation="MTVPAGPMRLGELLPLAHSFADAVVDSAVKEVEAHGEKISCKAG
                     CGACCRQLVPIAEAEARRLGALVQRLPEPRRETVRARFAAARDRLEKAGLLERLRHPD
                     GWSDGEGRSIGQAYFREGIPCPFLEEESCSIYGDRPIGCREYLVTSPADNCARPTAET
                     VARVKLPLKVWTAIARFDEVPPRSHFIRWVPLILAPAWAEAHPDEPASRPGPEMLREF
                     FDHLTGKRHPQRGSESSPFDPTLVAASPDGEAGP"
     gene            complement(1151..2734)
                     /locus_tag="AC16G0_15735"
     CDS             complement(1151..2734)
                     /locus_tag="AC16G0_15735"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HIR5101105.1"
                     /translation="MTWSEPNPYDTVRYGGHALAQAHPDRLATIATLFGMKPPAVFRC
                     RILELGCGDGLNLISVGLGLPEAECVGIDLAASGIARGQEILQSIGLKNVTLSQRSIL
                     DIDPDFGQFDYIIAHGVYSWVPPEVQDKLLGICHENLAENGVAYVSYNAYPGSHQREM
                     VREMMRYHVAGFEDPAQRIEQARALVEFLAEQARTEGDPYRSVLKQERERIRGFADSS
                     LFHDDLAEENAPVYFYQFVEDAARHGLQYLSEAHFTETQAGVFPASVVAVLNQIADSL
                     IAKEQYLDFLKGRRFRQTLLCHAACELDRIPRPERMAQFYLASPLRTDSPLSDQQSRA
                     IVAFAGPLKSSIGTDSPLIKAALAALGEAWPQAVHFHDLLAVARAACGRGNGPDGASM
                     DEDIRVLGDLLLRAYAGGIIELYVHPPRFVVQPGERPVVSPLARLQSREDGKVINLRH
                     YNIEVDNPVGRYLLRRLDGSLDRAGLVDDLAALVDSGDLRIQTGEASVQDGRSVRELL
                     TAELERQLKDLGERALLVA"
     gene            complement(2830..>3639)
                     /locus_tag="AC16G0_15740"
     CDS             complement(2830..>3639)
                     /locus_tag="AC16G0_15740"
                     /inference="COORDINATES: protein motif:HMM:NF018699.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HIR5101106.1"
                     /translation="RLMTQARALFDAGQLNAAIGELTRDVKAHPADTSLRTFLFELLC
                     FAGEWDRAEKSLDVLGHQDMKAEIGVQVYRNNIKAERDRRKLFSDGLQPHFLTEPPAY
                     VDLHLAAINRLREGNTGEAREALDRAEAERPALAGTFNGRRFADFRDWDDLVGPVLEL
                     IIKDQYTWLPYEQIGRMEVNPPKQARDLVWANARIQATDGTIGEVYIPALYAGSSEHG
                     SDQVRLGRMTDWKRLSDDVSVAVGLRLFLVDDEDKPIFEARSVEFEPVKKP"
BASE COUNT          636 a         1280 c         1102 g          621 t
ORIGIN      
        1 ctccaaaatc cgccgaatcc gcgcgcgatc cttctcgccc agaaggctga acgcggtgaa
       61 gtccggaggg ccatagttga ccagcgatcg ggagacctcc acgaaatccg ggggcaactc
      121 ctcgagcatc tcccggcggg tgttgagcag ttcctctagg tcccgggcca ccgaccgctc
      181 caactgccgc accgtctggg tgcgaattgg gatggcttcc gccgacacct ccggcctgtc
      241 gtccagcaat cggtccagaa tcgagggcac aatattcggt tcccggtcag ctcggggcat
      301 cagggacctg cctctccatc gggcgatgcc gcaaccaggg tcggatcgaa aggcgaagac
      361 tccgatcctc tctgggggtg tcgcttcccg gtcagatggt cgaagaactc gcgcaacatc
      421 tccggcccag gccgggaggc cggctcgtca ggatgagcct ccgcccacgc cggggccagg
      481 atcaacggca cccagcggat gaagtgggac ctgggcggga cctcgtcgaa gcgggcaatc
      541 gcggtccaca ctttgagcgg cagcttgacg cgcgccaccg tctccgcggt cgggcgggcg
      601 cagttgtccg ccggcgaggt caccaggtac tcgcggcagc cgatcggccg atccccataa
      661 atggaacagg actcctcctc caggaacggg cacgggatcc cctcgcgaaa atacgcttgc
      721 ccgatggatc ggccttcccc atcggaccac ccgtccgggt ggcgcagcct ctccagcagg
      781 ccggccttct ccagccggtc acgggccgcc gcaaagcggg cgcgcaccgt ctcccgccgg
      841 ggctcaggaa gacgctgcac cagcgcgccc aggcgccggg cctcagcttc ggcgatgggc
      901 accagttgcc tgcagcaggc gccacaccct gccttgcagg agatcttctc cccgtgcgcc
      961 tcgacctcct tgaccgccga gtccaccacg gcgtccgcga aggagtgggc cagaggcagc
     1021 aactcgccga gccgcatcgg ccctgccggc acggtcatcg ttgtccgaag ctcccagtcg
     1081 gggccagcca atcggacttg cgcagtgacc gacccgggac aaccgagtga gtcctttgcc
     1141 atttccgcct tcaggcaacg agcagtgccc gttcccccag gtccttgagc tggcgttcca
     1201 gctcggcggt cagaagctcc cggacacttc tcccgtcctg gacagaggcc tctcccgtct
     1261 ggatgcggag atcgcccgag tccaccaagg ctgccagatc gtccaccagg ccggcccggt
     1321 ccagcgaccc gtcaagacgt cgaaggagat atcgccccac gggattgtcg acctcaatgt
     1381 tgtagtggcg gagattgatc accttgccgt cttcccggct ctggaggcgc gcaagcgggc
     1441 tcaccacggg ccgctcgccg ggctgcacaa caaaccgcgg ggggtggaca tacagctcga
     1501 tgatgccccc tgcgtatgcc cgcaacagca ggtcccccag cacacggatg tcctcgtcca
     1561 tgctggcgcc atccggcccg ttcccccgcc cgcaggctgc tcgcgccacc gccagtaggt
     1621 cgtggaagtg caccgcctgc ggccaggcct ctcccagggc cgccagcgcc gccttgatca
     1681 aggggctatc cgtgccgatc gacgacttca gcggcccggc gaaggcaacg atggcacggg
     1741 attgctggtc ggatagaggg gaatccgtcc gcagcggcga tgcgaggtag aactgggcca
     1801 tccgctccgg tcgcgggatg cgatccagct cgcacgcggc gtggcacaag agggtctgcc
     1861 ggaaccgcct cccctttaag aaatcgaggt actgctcctt ggcgatcagg ctgtcggcga
     1921 tctgattgag cacggccaca acactcgccg ggaacacccc cgcctgcgtc tcggtgaaat
     1981 gcgcctccga gaggtattgc agtccgtgcc tggctgcgtc ttcgacgaac tggtagaaat
     2041 agaccggggc gttctcctcg gccaggtcgt catgaaagag cgaactgtcg gcgaaccccc
     2101 ggatccgctc tcgttcctgc ttgaggaccg accggtacgg atcgccctcg gttctggcct
     2161 gctccgccag aaactcgacc agcgcccgcg cctgttcgat ccgctgggcg ggatcctcga
     2221 aaccagccac atggtagcgc atcatctcgc gcaccatctc gcgctggtga gagccggggt
     2281 aggcgttgta gctcacgtag gccacgccat tctccgccag gttctcgtga cagatgccga
     2341 gcagcttgtc ctgcacctcc ggcggcaccc aggagtacac cccatgggcg atgatgtagt
     2401 caaattgacc gaaatccgga tcgatgtcga ggatgctccg ctgggagagg gtcacgttct
     2461 tcagaccgat actctggagg atctcctgtc ctctcgcgat cccggacgct gccaggtcga
     2521 tgcccacaca ctccgcctcc ggcaagccca gaccgaccga gatcaggttc agcccgtcgc
     2581 cgcatcccag ctccaagata cggcagcgga ataccgccgg cggcttcatg ccgaagagcg
     2641 tggcgatcgt cgccagccga tccggatgag cctgcgccag cgcatggccg ccgtagcgca
     2701 ccgtgtcata cggattgggt tccgaccaag tcatcgccat cccatccacg ccatttgtct
     2761 cggcgtgtgg aaatctcccg cagcctgacc tgatccggcc ggacccccag atggaagcac
     2821 gttcgatcgc tacggcttct tcacgggctc gaactcgacc gagcgcgcct cgaagatggg
     2881 cttgtcctca tcgtccacaa gaaacagacg caggcccacc gcaaccgaca catcatcgct
     2941 cagccgtttc cagtcggtca tccggccgag gcggacctgg tcgctcccgt gctcgctgga
     3001 gcccgcgtac aacgccggga tatacacctc cccgatcgtg ccgtccgtcg cctggatccg
     3061 ggcattggcc cacaccaagt cgcgggcctg cttgggcggg ttgacctcca tccgtccgat
     3121 ctgctcgtag gggagccagg tgtactggtc cttgatgatc agttcgagga ccggcccgac
     3181 caggtcgtcc caatccctga agtccgcaaa ccgcctgccg ttgaacgtgc ccgccagcgc
     3241 gggccgctct gcctccgccc ggtcgagcgc ttcccgcgcc tcgcccgtgt ttccctctcg
     3301 gagacgattg atcgccgcga ggtgcagatc aacgtaggcg ggcggctccg tgagaaagtg
     3361 cggctgcagg ccatcggaaa acagcttgcg gcggtcacgc tcggccttga tattgttccg
     3421 gtagacctgc acgccgatct cggctttcat atcctggtga ccgagcacat ccagactctt
     3481 ctccgcacga tcccattccc ccgcgaagca cagcagttcg aagagaaatg tgcgcaacga
     3541 ggtgtctgct ggatgcgcct tcacgtcgcg cgtcagctct ccaatcgccg cgttcagttg
     3601 gcctgcatca aacaacgccc tggcttgggt catgagcct
//
LOCUS       DBNGZC010000910         2961 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_565162, whole genome shotgun sequence.
ACCESSION   DBNGZC010000910 DBNGZC010000000
VERSION     DBNGZC010000910.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_565162"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..1485
                     /locus_tag="AC16G0_15745"
     CDS             307..1485
                     /locus_tag="AC16G0_15745"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5101107.1"
                     /translation="MRPSLTCQGMLGVVAFLLLAGVGLAGAATPAAMDAWLKQAEMGP
                     YQEAKFDADTLYQKAKGEGKVTVYSYSSRIVEVAKTFEQRYPGIKVQGFDMDSAEIVT
                     KVQAEQSAKNYAADVVYMKDPATALNALKTPGHLVNYVPGDLVSLLRPEHREPFLIHH
                     LSFDVLVYNSTGQAKAPVSSLWDLTRPEWQGRVLFPDPQVLPEFTEVLATIVQHSPEM
                     AKEYERVFGKPLQLSPGVPDAGREWIKRLLANKATVARSTNDVAKAVGRAEPTKAPIG
                     LTAMSRLRDKEKDPKLQFDIAYDLQPVGAVTTGVLLAVPSYATHPNAAKLLIRWLMGD
                     AKGGEGYAPYFVLGDAPTRSDQPLPSGMRAIDRRWYADPAYVWANGRAILDFWVANLK
                     "
     gene            1518..>2961
                     /locus_tag="AC16G0_15750"
     CDS             1518..>2961
                     /locus_tag="AC16G0_15750"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5101108.1"
                     /translation="MESGATLPLRARLNRVAYYLRLPRVGLGMGLFAVLLVLVTLPCL
                     KLLAATLTWDAADIRLSPAAQPGALTLFHWARVFASDLSAALFYRPLFHSLVTSVSIG
                     LLSVGLGTGMAWLTTRTDLPGRGLVAAMATVPYILPSYTYALAWLTVFRTERLGGVAG
                     LFRFIVGADPPEWLAYGFVPIVVTLTLHYAPFTYLLVAGALASLDAQMEEAGEVLGAS
                     RATILRWITLPLVLPALLSGFILTFSRALGTFGTPYFLGAPVRYFTLPTTIYSSMVTR
                     TFATGYILAVVLIAVSALVIYANQRVIGTRKSYVTIGGKGFRTVQIHLGAWRFPAALA
                     SWGLVLVAVAIPLVLLAWQTLMRYPDDYSLGNLTLHYWLGASDPRFAEGELGILRNSS
                     ILGGAWNSIKLSVIVAALGGLLGILIGYVVVGGRGGCLARLLEQQAFLPYLIPSIAFG
                     AIYLSLFTTPIGPIPSLYGSFLLVAMVCIAK"
BASE COUNT          463 a         1003 c          927 g          568 t
ORIGIN      
        1 gcgctcggcc ccggtggata tcttcccgct gacgcttttc ccaaagctcg attaacccgt
       61 gatccattct cgccactccg tgggtatcct ccatacccgc ctggctcctc cgtctctgcc
      121 tcccgtacag aagctgtgtc cgaaccttac cccttattcc cccagggctg ccccagggct
      181 ggatgttgaa aggctcgccg gtcctggacg gcttgacatt cccggaccat ccctgcgaat
      241 attgcgggcg aggcgcacga gtattcatac gatcagatga ccaacgctcg agaagggagg
      301 cataatatgc gtccgtcact gacctgccag gggatgcttg gggtggtcgc gttcctgctc
      361 ttggccgggg tcggcctggc cggcgcggcg acacccgcgg caatggatgc ctggctcaag
      421 caggcggaga tgggaccgta ccaggaggcg aagttcgatg ccgacacgct gtaccagaag
      481 gccaagggcg agggaaaggt aaccgtctac tcctattcgt cccggatcgt ggaggtggcg
      541 aagaccttcg aacagcggta tccgggaatc aaggtccagg gctttgacat ggactcggcg
      601 gagatcgtga cgaaggtcca ggcggaacaa tcggccaaga attacgcggc agatgtggtg
      661 tacatgaaag atccagccac ggccctgaac gcgctgaaga cccccggtca tctcgtgaac
      721 tacgtccctg gcgacctggt ctccctcctc cggcctgagc atcgagagcc cttcctgatt
      781 caccacctga gcttcgacgt cctggtctac aactcgaccg gtcaggcgaa ggccccggtg
      841 tcaagcctat gggatctcac caggccggaa tggcaggggc gggtgttgtt ccctgacccc
      901 caggtgctgc ccgagttcac cgaagtcctg gccaccatcg tccagcacag ccccgaaatg
      961 gccaaggagt acgagcgggt cttcggcaag ccgcttcagc tcagtccggg agtgcccgat
     1021 gcggggaggg agtggatcaa gcgcttgctg gccaacaaag cgacggtcgc cagatccacc
     1081 aacgatgtgg ccaaagcggt gggccgggcg gaaccgacca aggcccccat cggattgacg
     1141 gccatgtcgc gcctgcggga caaggagaag gacccgaagc tccaattcga catcgcctat
     1201 gacctccagc cggtgggtgc ggtaaccacc ggggtcctgc tggcggtccc ctcctacgcc
     1261 acgcatccca atgcggccaa gctgctcatc cgctggttga tgggtgatgc caagggcggc
     1321 gaagggtacg cgccctactt tgttctggga gacgctccca cgcggtcgga ccagccgctg
     1381 ccgtcgggga tgcgtgccat cgatcgtcgc tggtatgcag acccggccta cgtctgggcc
     1441 aacgggcgtg caatcctcga cttctgggtg gcgaacctga agtaagcgcc cccgctggct
     1501 tgcagcctgg agatcggatg gaatcggggg cgaccctgcc gcttcgcgcc cgcttgaacc
     1561 gcgtggccta ttacctgcgt ctgccgcgcg tcggactggg catgggcctc ttcgcggtcc
     1621 tgctggtcct ggtgaccctt ccgtgcctga agctgttggc cgccaccttg acctgggatg
     1681 cggcggacat ccggctgagc cccgcggccc agcccggggc cctcaccctg ttccactggg
     1741 cgcgcgtctt cgccagcgat ctcagcgccg ccctcttcta ccggccgctt tttcattccc
     1801 tggtcacctc ggtgagcatc ggcctcctgt cggtaggcct cgggaccggg atggcctggc
     1861 tcacgacccg gacggatctg ccgggccggg gtctggtggc ggccatggcc accgtcccct
     1921 acatccttcc gtcgtacacc tatgccctgg cgtggctcac cgtgttccgg acggagcgtc
     1981 tgggcggcgt ggccggcctg tttcgtttca tagtgggcgc cgacccgccg gagtggctgg
     2041 cctacgggtt cgtgccgatc gtcgtgacgc tgacgcttca ctatgcgccc ttcacctacc
     2101 tcctcgtggc cggggccctg gccagcctcg acgcacagat ggaggaggcg ggcgaggtcc
     2161 tgggagcctc ccgggccacg atccttcgat ggatcaccct tcctctcgtc ctcccggccc
     2221 tgctgtcggg attcatcctg acgttctccc gcgccctggg gaccttcggg acgccgtact
     2281 tcctgggggc gccggtccgc tacttcacgc tgcccacgac gatctactcc agcatggtga
     2341 cccggacctt cgccaccggg tacatcctgg cagtcgtcct gatcgccgtc tcggccctgg
     2401 tgatctatgc caaccagcgc gtcatcggca cccggaagag ctacgtcacc atcggcggga
     2461 aggggttccg gaccgtccag atccacctgg gggcctggcg tttcccggcc gccctggcga
     2521 gctggggtct cgtgctcgtg gccgtggcga tccccctggt gctcctcgcg tggcagaccc
     2581 tcatgcggta cccggacgat tactccctgg gaaacctgac gctccactac tggctggggg
     2641 ccagtgatcc ccgttttgcg gaaggggaac tggggatcct ccggaacagc agcatcctgg
     2701 gaggggcgtg gaacagcatc aagctctcgg tgatcgtggc cgcgctgggc gggctcctgg
     2761 gcatcctgat cggctacgtg gtcgtgggag gacggggcgg ctgcctggcc cggctcctgg
     2821 aacagcaggc attccttccg tacctcatcc ccagcatcgc cttcggggcc atctatctct
     2881 ccctcttcac caccccgatc ggacctatcc cttccctcta cggaagtttt ctccttgtcg
     2941 ccatggtctg catcgccaag a
//
LOCUS       DBNGZC010000911         1844 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_959676, whole genome shotgun sequence.
ACCESSION   DBNGZC010000911 DBNGZC010000000
VERSION     DBNGZC010000911.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_959676"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1514
                     /locus_tag="AC16G0_15755"
     CDS             join(124..334,334..1514)
                     /locus_tag="AC16G0_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611780.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
                     /protein_id="HIR5101109.1"
                     /translation="MAPSPLTPQQQAEIEDLSRAIREAVDAEIDELAANLTTTDDAHL
                     FGDNEFKIRALAHKIAAKAVEQRLARKKNGYEGAGVTCPHCDQAAEFHAHRWHTSYSL
                     VGPIRYRRAYYLCRRCGKGLFPFDREADLTSRDLTPALERVATLSGAVADSFEKGAEL
                     LEEMAGTRLSESTVERRTEDAGQRLAELVAVGVPLGPRVDWPWYKDYEGKTCAYVELD
                     ATGVRQQGAGGAAAEGRMAYVGMVCNPSPEWPWPDEKPRPMRARYLAGLYPLEQFGPL
                     LRTPAGHVGLDRADRWIGLSDGGNGLEDRLRENFPRVEVVILDFFHPAEKLTALARLL
                     HPDDEDRAEDQARQWRCLLKEEGGAVLAAVLSEWEWPRRAGVSEAASEVIGYLERNAH
                     RMEYPEYLAKGWCIGSGAVESACKTVVGQRLKLAGMRWGQDGADALCHLRALYRSEKG
                     QWDAFWRRDFSRN"
BASE COUNT          346 a          585 c          611 g          302 t
ORIGIN      
        1 ttagcgaaat ccctcacagt agaagtaatc ggtgggcgtc ttgaaagttg gtggaatctt
       61 tgtacacaag agcgaaatcg gtgaccgttg aggagaatcc gaccccggaa acggagccct
      121 gccatggcac cctcaccgct gacgccccag caacaggccg agatcgagga cctctcgcga
      181 gccatccgcg aggcggtcga tgccgagatc gacgaactgg ccgccaacct caccaccacc
      241 gacgacgccc accttttcgg cgacaacgag ttcaagatcc gcgccctcgc ccacaagatc
      301 gccgccaagg cggtcgagca gcgcctggca cgcaaaaaaa cggctacgaa ggcgccggcg
      361 tgacctgccc gcactgtgat caggccgccg agttccacgc gcaccgctgg cacacctcgt
      421 acagcctcgt ggggccgatc cgctaccgcc gcgcctacta tctgtgccgg cgctgcggca
      481 agggcctgtt ccccttcgac cgcgaggcgg acctgacgag ccgtgacctg acaccggccc
      541 tggagcgggt cgccaccctg tctggagcgg tggccgacag cttcgagaag ggggccgaac
      601 tgctcgaaga aatggccggt acccgactct cggagtcgac tgtcgaacga aggaccgaag
      661 atgccggtca gcgtttggcc gaactcgtcg cagtcggtgt gcccctcggc ccccgagtcg
      721 actggccgtg gtacaaagat tatgaaggaa agacctgcgc ttatgtcgaa ctggacgcca
      781 ccggcgtgcg ccagcaaggc gcggggggcg cggccgccga gggccgcatg gcctacgtgg
      841 gcatggtctg caacccgtct ccggagtggc cctggcccga cgagaaaccg cgaccgatgc
      901 gggcacgtta tctcgcgggg ctgtatcccc tggagcaatt cggcccgttg ctccgcacgc
      961 ccgccgggca cgtcggtctg gatcgggccg accgctggat cgggctctcg gatggtggca
     1021 acggcttgga ggaccgactc cgggagaact tcccgcgcgt cgaggttgtc atcctggact
     1081 tcttccatcc cgcggagaaa ctcaccgccc tggcgcggct gctgcatccc gacgacgaag
     1141 accgagccga agatcaagca cggcaatggc ggtgtctgct gaaggaggaa ggcggcgcgg
     1201 tgctggcggc ggtgctgagc gaatgggagt ggccgcgtcg tgcgggcgtg agcgaggcgg
     1261 ccagcgaggt gatcggttat ctggagcgga acgctcaccg catggagtat cccgaatacc
     1321 tggccaaagg ctggtgtatc ggcagcggcg cggtggagag cgcgtgcaag accgtggtgg
     1381 gccaacggct gaaactggcc gggatgcgct ggggccaaga cggcgccgac gccctctgcc
     1441 acctccgcgc tctgtaccgc agtgagaagg gtcagtggga cgccttctgg agacgtgatt
     1501 tctcccggaa ctgatcgcct gtatcaccaa ctaacgtgac gctcaccggc tggacccagt
     1561 gctggaccat ggactgactg acgtggaact gacgggcagc atcgtgttgg gattgccccc
     1621 gtcggacggc ggcgacgatg gcccgacgac gagtggcttc ccggtctctt tgctcatgat
     1681 gaccaccggg agaggatgcg gtttttcagt ccagcttaca cccgagtgat cgggatcatg
     1741 tggcacaaaa ctgatcggga tcatgtggca cttggccctt gaacacctaa acccccttct
     1801 gcgcctcgat gtagcgccgg atggtctctt gcgagacgtt gccc
//
LOCUS       DBNGZC010000912         2968 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_761924, whole genome shotgun sequence.
ACCESSION   DBNGZC010000912 DBNGZC010000000
VERSION     DBNGZC010000912.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_761924"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1491)
                     /locus_tag="AC16G0_15760"
     CDS             complement(<1..1491)
                     /locus_tag="AC16G0_15760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HIR5101110.1"
                     /translation="MRFGLKAKETLVITLLSFLVVATTSVLHLSQLARVTVLESLRQG
                     ELMARQIYAQGSRALARGQGGTPWEILKRDPELRSLIEASVGYSPELVYVFLADQSGR
                     AILHSQPDKEGSEIPPRPTLEKLVGYDALQRFWALYSGDAVYEATLPLNLNGRPFGSV
                     RLGFSTSLLRRELNSSLQRGLVLAGLSLPLAWLVAMVLANLTLKPIRKFTRQMERMRQ
                     GEFEVSSDLAQGDEFKELASELQLLGQQLQSDRLKMLGERLDLHQVVDQLEDGLIFFN
                     QDHRILFINKAAETVLGRGVEGVVGRRLEEVLESSHPLRMLMERVFSQSVGSRNVTIS
                     LPRNEKSREFLVSAFSVRDVHKTMGAMVLLKDLDSLKTLQSLVSYSAKLTALGRLTSG
                     VAHEVKNPLNAMMIHLELLKEKLGPSSEGVQENLEVIGNEIRRLDRALQGFLKFIRPQ
                     ELALKPVELNTLLQSLIALLEVEWQKTGVRFVFRPESPLPTITADEE"
     gene            complement(1494..2846)
                     /locus_tag="AC16G0_15765"
     CDS             complement(1494..2846)
                     /locus_tag="AC16G0_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR5101111.1"
                     /translation="MAHRILIADDDESSRQGLKMLLSGAGYEVEETSDGQAALDKALT
                     FLPTVVVTDLVMPRMDGLGLLKALRAELPFATVIVLTGHATVETAVAALKEGAYDYLT
                     KPVDIPRLRILIEKALEKGEALREVTVLRRRLKDVWGIGKLVGKSPGMQEIYRLIDLA
                     APTSAPVLVTGESGTGKELVARSLHDLSPRSKGPFVAVNCSAIPETLLESEIFGHEKG
                     SFTGALERRAGCFELAHEGTLFLDEIAEMTAATQAKFLRILQDGTVRRLGGKVEMQVD
                     VRVVAATNKDPLKALKDGVFREDLYYRLNVFCIALPPLRDRRDDIPLLVQAFLEEFNG
                     KYEKRVRSVDEAVLQILLAHPWPGNVRELRNTLERAIIICEGELIAPKHLPPDLTHAT
                     GEERADVLTFPLGVTVEEAEKTLILKTLASVSNNKAKAASILGISVKTLHNKLHRYGV
                     "
BASE COUNT          516 a          967 c          916 g          569 t
ORIGIN      
        1 ttcctcatcg gcagtgatgg tggggagggg agattccggc cggaagacga accggacccc
       61 cgtcttttgc cattccacct ccagaagggc gatgaggctc tgaaggagcg tgttcaactc
      121 gaccggcttc agcgccaact cctgcggacg gatgaatttc aggaacccct gcagcgctcg
      181 gtccagccgt cggatctcgt tgccgatgac ctccaggttc tcctgcaccc cttccgacga
      241 tggcccgagc ttctccttga gaagctccag atggatcatc atggcgttga gcgggttctt
      301 gacctcgtgg gccaccccgg aagttagtct gcccagcgcc gtcagcttgg ccgagtagct
      361 cacgagggac tggagcgtct tgagggaatc caggtccttc aagagcacca tggcccccat
      421 ggtcttgtgg acgtccctca cggaaaatgc cgagaccaga aactcgcggg acttttcatt
      481 ccggggcagg ctgatggtga cgtttcggga tccgacgctc tggctgaaga cgcgctccat
      541 gagcatgcgc aagggatggg aggactcgag gacctcttcc aggcgccgcc ccaccacccc
      601 ctccacgccc cttcccaaga cggtctccgc cgccttattg atgaagagaa tccggtggtc
      661 ctggttgaag aagatgagcc cgtcctcgag ctgatccacc acctggtgca ggtctagtcg
      721 ttcgcccagc atcttcaggc ggtccgattg gagctgctgg cccaggagct ggagctccga
      781 ggccagttcc ttgaactcat ctccctgggc gagatcgcta ctgacctcga attctccttg
      841 gcgcattcgt tccatctgcc gagtgaactt ccggatcggc ttcagggtca agttggccag
      901 aaccatggcc accagccatg ccagcggcag agataatcct gccaggacga gaccccgctg
      961 cagggaggag ttcagctctc gccgcaggag gctggtggag aatcccagcc ggacgctgcc
     1021 gaacggacgg ccgttgaggt tcaggggcag cgtggcctca tacaccgcgt ccccactgta
     1081 gagcgcccag aagcgctgca aggcgtcgta gcccacgagt ttttcgaggg ttggccgcgg
     1141 cgggatttcc gacccctctt tgtctggttg gctgtggagg atggcccgac ccgactggtc
     1201 ggcgaggaag acatacacca gttcgggtga gtagccgacg ctcgcctcga tgaggctccg
     1261 caactccggg tcccgcttca aaatctccca gggggttccg ccctggcccc gcgcgagggc
     1321 ccggctgccc tgcgcgtaga tttgcctcgc catcagctcg ccctgccgca ggctctcgag
     1381 gacggtgacc cgcgccaact gggagaggtg gaggacgctg gtcgttgcca ccacgaggaa
     1441 gctgaggagg gtgatgacca gggtctcttt ggctttcagg ccgaaacgca cacctatacc
     1501 ccgtaccggt ggagcttgtt gtgtagcgtc ttgacgctga ttcccaggat ggaggccgcc
     1561 ttggccttgt tgttgctgac ggaggccagg gtcttgagga tgagggtttt ctccgcctct
     1621 tcgacggtga cacccagtgg gaaggtcagg acgtcggcgc gttcctctcc cgtggcatgg
     1681 gtgaggtccg gaggcaggtg cttgggggca atcaactcgc cctcgcagat gatgattgcg
     1741 cgttcgagcg tgttccgcag ctctcgcacg tttcccggcc aaggatgcgc caggaggatc
     1801 tgcaggaccg cctcgtccac cgaccggacc cgcttctcgt acttgccgtt gaactcctcg
     1861 agaaacgcct ggacgagcag ggggatgtca tcgcgccggt ctcgaagggg gggcagggca
     1921 atgcaaaaga cattcaggcg gtagtacagg tcctcgcgga ataccccatc cttcaaggcc
     1981 ttcaggggat ccttgttggt ggcggcgacc acgcgcacgt ccacctgcat ctccaccttt
     2041 ccccccaggc gccgcaccgt gccgtcctgc aggatgcgga ggaacttcgc ctgcgtggcc
     2101 gcggtcatct ccgcgatctc atccaggaag agggtcccct cgtgggccaa ctcgaagcag
     2161 cccgcccgcc gctccaaggc ccccgtgaac gatcccttct cgtggccgaa gatctcgctc
     2221 tccagcaggg tctccgggat ggccgagcag ttgaccgcga caaagggccc cttgctccga
     2281 ggggacaggt cgtggaggga ccgggccacc agctctttgc cagttccact ctctcccgtg
     2341 accaagacgg gcgcagaggt gggagcagcg aggtcgatca gccggtagat ctcctgcatc
     2401 cccgggctct ttcccaccag cttcccgatt ccccacacgt ccttcagccg gcgccgcagc
     2461 acggtcacct ctcggagggc ctcgcctttc tccagggcct tctcgatcag gatccggagc
     2521 cgcgggatat ctaccggctt cgtcagataa tcgtacgcgc cttccttcag ggcggccacg
     2581 gccgtctcga ccgttgcatg gcccgtgagg acgataaccg tggcgaacgg cagttcggcc
     2641 cggagcgcct tcaagagccc cagcccgtcc atcctgggca tgacaaggtc ggtcaccacg
     2701 acggtcggca ggaaggtcag ggccttgtcc agtgccgcct gtccgtcgga cgtttcctcg
     2761 acctcgtagc ccgcgccgga caggagcatt ttcagcccct gccgagagga ctcgtcgtcg
     2821 tcggcaatca ggattcggtg cgccataccc cctccttggg acgtatatca tactctaata
     2881 atgcaccatt ggtctccccg aaaattctgc gacccgcgag ggggttttcg cgaagtgcga
     2941 ggaggagacc ctccccccca ggatccca
//
LOCUS       DBNGZC010000913         3141 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_374663, whole genome shotgun sequence.
ACCESSION   DBNGZC010000913 DBNGZC010000000
VERSION     DBNGZC010000913.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_374663"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="AC16G0_15770"
     CDS             complement(<1..551)
                     /locus_tag="AC16G0_15770"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5101112.1"
                     /translation="MRDFGAQREGITLDGLVRMLRDGRITRRGFLVRAMGLAGSLAAA
                     EGLLARVAGAQTTTKNTLVVGQSADISKLDPHMSTTVNDIAITFNLFDNLLTRRRDGK
                     LYPSLASEWKLVNPTTWQFKLRPNVKFHNGDRLTSADVKFSFERSYDPNTKSITKSIL
                     TTIAQIDTPDPLTVVIHTKQPDPL"
     gene            899..1765
                     /locus_tag="AC16G0_15775"
     CDS             899..1765
                     /locus_tag="AC16G0_15775"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5101113.1"
                     /translation="MWILLALAAALFQVLRNTVMKRLGHSLDEYINVWGRFTFLLPFA
                     LILALAHGLPSIQPGFVSICLLFGLCQTVSTLALSKALKLSQISLVTALWKLSLPILL
                     LMGFLTLKETPTPIGIAGVLLSAAGVYLLNVSHARLSPWAPLTILFRDPGLRYTLLSA
                     LFFAPSVVTIKQAILLSDPYTGTFGGYLAACLWVTPLVLVTSRPQFAAVPHHWEEFAG
                     LGLFAALTTVSQGIAYTLTLSSYVEAVKQVEILLALLIGGLAFGERQTAREVAPGACV
                     MLAGVVLLSLAG"
     gene            1931..>3141
                     /locus_tag="AC16G0_15780"
     CDS             1931..>3141
                     /locus_tag="AC16G0_15780"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5101114.1"
                     /translation="MSLEPPTIRPDGRRRFIVVGTTFITLGLVLGVWYSYSVFLVAFL
                     QEFGWKRSLVAGAFSIFVLVHGSVSPIVGWLTGRIGTRRVILLGSCVLACDLLLTAET
                     REWWHLYLAFGGVVAVGLTMAGWIPAVVLVRGWFPNRFGTAIGITSAGIGVGIFAVVP
                     FAQLLIDRVGWRWAFRVLAGVIVGWVLPATAALIRESSTPTVSTSASPRRHRPVFSNT
                     GAYWTLATAIRDWHFWGLAGVFFTGNFVTQMLLVHQVAYLVDHGVTPLAAAAVGGLAG
                     LTSILGKTVCGVLSDRAGREPAYGLSILCVVASLGALVLAGRYPSSGLPYLYAVLIGL
                     GYGGIAPLAPAAASDLFSGPAFSTIFGAVYPAMCLGIASAAWLAGRIFDGTGGYALAL
                     WIALLMAILSPV"
BASE COUNT          433 a         1019 c         1014 g          675 t
ORIGIN      
        1 agcggatcgg gctgcttggt gtgaatgacg acggtgagcg gatcgggggt gtcgatctgg
       61 gcgatggtgg tcaggatgga cttggtgatg ctcttggtgt tcgggtcata agagcgctcg
      121 aagctgaact tcacgtccgc ggaggtgagc cggtccccgt tgtggaactt cacgttgggg
      181 cgcagcttga actgccaggt ggtcgggttc acgagcttcc actcggacgc cagggagggg
      241 tacagcttgc cgtcgcgccg gcgcgtgagc aggttgtcga acaggttgaa ggtgatggcg
      301 atgtcgttga cggtggtgct catatgggga tccagcttgc tgatgtcggc ggactgcccg
      361 accaccaggg tgttcttggt ggtcgtctgg gcgccggcca cccgcgccag cagtccctcg
      421 gcagcggcca gactgccggc gaggcccatg gcccgtacga ggaatccgcg tcgggtgatt
      481 cgcccatccc ggagcatccg gaccagaccg tccagcgtga tgccctcgcg ttgtgctccg
      541 aagtctctca tgacggggcc tcctttccag cagggggtga agtgccattc ggcgactggg
      601 agccgtcccg gctcgccagt gggagtggtg ggctatgtga cgggtggccg atgggcgtcc
      661 cgaggtgtcc atgcataccg gaatcgccgc ctagaaacta ttcatgaccc cggtggttgt
      721 caagtcaatt ggaggcaatg tcaatcggga gtcaccatcc atccgggaaa atgtctttgg
      781 cggtttctcc ttctaaggct atcgtgtcgc atcgcgtcta gacgagtgga cagggaggcg
      841 gcagcaggct gcacctcgcc gcccccatcg gtcagcattc ctgtgagagg ctgttgaggt
      901 gtggatcctc ctcgccctgg ctgcggccct gttccaggtc ctccgcaaca cggtcatgaa
      961 gcgcttgggg cactccctgg acgagtacat caacgtctgg ggccgcttca cctttctcct
     1021 gcctttcgcg ctgatcctgg ctcttgccca cgggctcccc agcatccagc cggggttcgt
     1081 ctccatctgc ctgctcttcg gcctgtgcca aaccgtttca accttggcgc tgtcgaaggc
     1141 gctgaagctt tcgcagatct ccctggtcac cgcactctgg aagctgagcc tccccatcct
     1201 gctcctcatg ggcttcctga cgctcaagga gacgccgacc ccgatcggaa tcgcgggcgt
     1261 cctcctctcg gcggcaggcg tctacctctt gaatgtgagc catgcccgcc tctctccctg
     1321 ggcgcccctc acgatcctct tcagggatcc gggcctccgc tacacactcc tctcggccct
     1381 gttcttcgcc ccctcggtcg tgacgatcaa gcaagcaatc ctgctctccg acccgtacac
     1441 ggggaccttc ggcggctacc tcgcggcgtg cctctgggtc acccccctgg tcctcgtgac
     1501 ctcgcgtccg caatttgcgg cggtgccgca tcactgggag gagttcgccg gactcgggct
     1561 cttcgccgcc ctcaccaccg tgtcccaggg gatcgcttat accctcaccc tctccagtta
     1621 cgtggaggcc gtgaagcaag tggaaatcct cctggccctc ctgatcggcg gtctggcctt
     1681 cggtgaacgg cagacagcca gagaggtcgc gcccggcgcc tgcgtcatgc tggccggggt
     1741 ggtgctcctg agcttggctg gctgaccata cagcccatct tccccattcg acatcaccca
     1801 ggtttttcgg catagttccc tgcggtcaag gcgggggaac cgaccccgcg catccccgac
     1861 accagttctc gccgcggggc ctccctcgac tctgcggaca cggtcggcag gctatgcccg
     1921 gctttcaacc atgagccttg agccaccaac catacgtccc gatggccggc gccggttcat
     1981 cgtcgtcggg acgaccttca tcaccctggg tctggtcttg ggcgtctggt attcctactc
     2041 ggtgttcctt gtcgcctttc tgcaggagtt tgggtggaaa cgatccctcg tcgccggagc
     2101 cttctcgatc ttcgtcctgg ttcacggttc cgtgagcccc atcgtaggat ggttgaccgg
     2161 gcgcatcggg acgaggcggg ttatcctcct gggaagctgc gtgttggcat gcgacctgct
     2221 cctcacggcg gagacgaggg aatggtggca cctctacttg gcgttcggcg gggtcgtggc
     2281 ggtgggactt accatggccg gctggattcc ggcagtggtg ctggtacgag gctggttccc
     2341 aaaccggttt ggcacggcga taggcattac ctcggccggg atcggcgtag ggatcttcgc
     2401 ggtggttccc ttcgcccagc ttctcatcga ccgggtcggc tggcgctggg ccttccgggt
     2461 tctcgccggg gtgatcgtgg ggtgggtgct gcctgcgacc gcggcgctca ttcgagaatc
     2521 ctccaccccg acggtctcga catctgcgtc cccccggcgc catcgccccg tgttttcgaa
     2581 taccggcgcc tactggacac tggccacagc catccgggac tggcatttct ggggcctggc
     2641 gggggtgttc ttcaccggga actttgtgac gcagatgctc ctggtgcatc aggtcgcgta
     2701 cctggtggac cacggggtaa ccccattggc ggcggcggca gtgggtggcc tcgctggtct
     2761 caccagcatc ctgggcaaga cggtgtgcgg cgtcctttca gatcgtgccg gccgggagcc
     2821 ggcctacggt ctatccatcc tgtgcgtggt cgcgagcctc ggcgccctgg tcctggcggg
     2881 caggtacccc tcctccggcc tgccctacct ctacgccgtc ctgattggac ttggatatgg
     2941 ggggatagcg ccgctcgccc ctgcagcggc cagcgatctc ttcagcgggc cggcattttc
     3001 gacgatcttc ggcgcggtct acccggcaat gtgcctgggg atcgcctcgg cagcctggct
     3061 cgcgggccgg atcttcgatg ggacaggggg ctacgctctg gccctctgga tagccctgct
     3121 gatggcgatc ctgtcgccgg t
//
LOCUS       DBNGZC010000914         2586 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_663676, whole genome shotgun sequence.
ACCESSION   DBNGZC010000914 DBNGZC010000000
VERSION     DBNGZC010000914.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_663676"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1291
                     /locus_tag="AC16G0_15785"
                     /pseudo
     CDS             128..1291
                     /locus_tag="AC16G0_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086730.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            1389..2378
                     /locus_tag="AC16G0_15790"
     CDS             1389..2378
                     /locus_tag="AC16G0_15790"
                     /inference="COORDINATES: protein motif:HMM:NF014653.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HIR5101115.1"
                     /translation="MQEVSSGRVRLSVPEARELAERALGRIGYDANEARIVADHVIDA
                     ALCGYEYSGLAKILNIPEHRRFKQPRRPMSVLHETEVSTLFDGGNNVGMLVMYHAARA
                     AIQKGCARGFALIGVTNSWVSGRSAYYVEMIAKADLIGIHTVSAARMVAPFGGIRPVL
                     GTNPIAFGMPSSRGPVVLDMGTSAIMATDLLLRERLGQLLPEGVALDPQGRPTRDPTQ
                     AKLGALLPFGGYKGFGLAFVMQALGVLAGSALDLEKDNGYLFVAFKPDLLIPLADFKR
                     QLSELIERVKSTPRQPGVEEIRIPSERAFRERNRNLRMGIEIDRRVYDALNAV"
BASE COUNT          450 a          790 c          895 g          451 t
ORIGIN      
        1 tgattcccgc gctgtgcgac ttgtggcgaa tgttcagcgt gattaacagt gcggttatct
       61 gctcgacgat gtgagcgttc tccagcgcac cgccatcgag ggcccgcaag ccagggattc
      121 tgtggacgag gtcctcgagt attacggtcg ggagatgatc cgcctgggga agccggtcaa
      181 gatgggggac ccccccggcg aggacaacag gtacttctac cagccccgcg gggtgggtct
      241 ggtcgtcgcc ccgtggaatt tccccctggc catctccgtc gggatgatct ctgccgccct
      301 ggtgacgggg aacgcggtcc tgtacaagcc gtcgagtcag tccctcggga tcgggtggct
      361 cgcctacgag atcctgacgc aggcaggagt gccgggcggc gtcctgaact tcgtcccggg
      421 ccgcggttcg gtcgtgggcg attatctcgt cgagcacaaa tacaccgact tcatcgtgtt
      481 caccggatcc ttggaggtgg gcttggggat cgtcgagaag gccggcagga cggtggcggg
      541 gcagaaagga cccaagaagg ttgtcatcga gacaggtggc aagaacgcca tcatcgtgga
      601 tggggatgcc gacctcgacc aggcggtgcc gggggtgatc cagtccgcct tagggttcca
      661 ggggcagaag tgctccgcct gctcgcgggt cctggtcctc gaggagtgct acgctcggtt
      721 ctgcgagcgt ctctccgagg cggtcaaggg gatcaccatc gggagccccg aggacccgaa
      781 gaatctggtg ggaccggtga tcgatggggc cgcgcagaag agcatattgg agtttatcga
      841 gaccggcaag aaagacggca gggtgctcgt ccaggttccg gcgccggagg caggctacta
      901 cgtgccgccc acggtcttcg tggacctgcc gccgacctcg agggtgatcc gggaagagat
      961 cttcgggccg gtgctctccg tcatgaagat gaagagtctc gacgaggcga tggaggtcgc
     1021 caactcctcg atctacgcct tgacgggcgg cctcttctcc cggagcccgg agaacatcga
     1081 tcgggtgcgc cgggagttct ccaccgggaa cctgtacatc aaccgcggca tcaccggcgc
     1141 catcgtcggt cgccagcctt tcggcgggtt caagatgtcg ggcgtcggtt ccaaggcggg
     1201 gggtccggac tatctccagc agttcatgga gccgcgcgtg gtgaccgaga acacgctgcg
     1261 ccggggcttc acgcccgagg atgtccagta gacaagatcc cgcggcggaa ttgggcggag
     1321 agacggcggg acgggcgtga atttcgggag ccgcgccccg ggatgcattc aacgagatac
     1381 ggagcgccat gcaggaggtt tcgtcaggca gagtgcggct aagcgtcccg gaggcgcgcg
     1441 agctggccga gcgagccctc ggccggatcg ggtacgacgc gaatgaggca cgcatcgtcg
     1501 ccgatcacgt gatcgacgcc gcgctgtgcg gctacgaata ctcggggctg gcgaagatcc
     1561 tcaacatccc cgaacaccgg cgcttcaagc agccccgccg tccgatgagc gtcctgcacg
     1621 aaacggaggt ctcgaccctc ttcgacggcg gcaacaacgt cggcatgctg gtgatgtatc
     1681 acgcggcgcg agcggcgatc cagaaggggt gtgcccgcgg gttcgcgctg atcggggtga
     1741 ccaactcctg ggtgagcggc cgcagcgcct actatgtcga gatgatcgcc aaggcagatc
     1801 tcatcggcat ccacaccgtc agtgctgcgc ggatggtggc gcccttcggg ggaatccgtc
     1861 ccgtcctcgg caccaatccg atcgccttcg gcatgccgtc ctcccgcggc ccggtggtgc
     1921 tcgacatggg cacgtccgcc atcatggcca ccgacctgct cttgcgcgag cggctggggc
     1981 agttgctgcc cgaaggcgtg gcgctcgatc cccaggggcg accgacgcgc gaccccaccc
     2041 aggcgaagct gggcgccctg ctccccttcg gcgggtacaa ggggttcggg ctcgcctttg
     2101 tcatgcaggc gctcggggtg ctggccggct ccgccctgga tctggagaag gacaacggct
     2161 acctgttcgt cgcgttcaag cccgacctgc ttattcccct ggccgacttc aagcggcaac
     2221 tgagtgaact gatcgaacgc gtcaagtcca cgccgcgcca gccgggtgtc gaggagatcc
     2281 gcatcccgtc ggagcgcgcc tttcgtgagc gcaaccggaa tttgcgcatg gggatcgaaa
     2341 tcgaccgcag ggtgtacgac gcgctcaacg ctgtttgaca tccgcccgta tcatgggagg
     2401 ctggccgctc ccaactcgat tcaggtcctc ttagagcgac gagccgcgct tgatcttgac
     2461 acggaggagg gccctgcgct gacgctgaat gccggcttca tatgatccgg gggatgccgg
     2521 tcgacaagca attgatgggc ctgggggaag cagtcccgac cgaagggagt gacgatgggt
     2581 acgaag
//
LOCUS       DBNGZC010000915         3384 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1738963, whole genome shotgun sequence.
ACCESSION   DBNGZC010000915 DBNGZC010000000
VERSION     DBNGZC010000915.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1738963"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="AC16G0_15795"
     CDS             complement(<1..1119)
                     /locus_tag="AC16G0_15795"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5101116.1"
                     /translation="MGRIPADYLPTGELRPTRIYTLPEYQHYPQRFNSTEELLDKQVA
                     AGKGDRIAIHFEDRKIPYKAVLATVNKLGSALRRLGIGEADRVMLRLPNIPPAVMANF
                     AAIKIGGVSLPTSALFSQAEIAHVSNLAEAKAVIVAGAMLEELEKARPNCKTIEHVIV
                     VGGTPEQQGAWKQKGYLLWQELIDAEKPECDPVRRDRMDVSVLLFTSGTTGLPKGTAH
                     FMEEALIVPDGFGKHCWNVSENDVIGGSAPIAMAAGYSTVSVIPYRFGAAVSLIARFT
                     PEAMFETIQNHKVTVLSILPTAYRKMLQVPDAEKKYNLSSLRICTGGGESLTAQTYYD
                     WKSKFGIEIFEGLGTTEMMYVFISSAATKRVKPGSIGPA"
     gene            complement(1137..1760)
                     /locus_tag="AC16G0_15800"
     CDS             complement(1137..1760)
                     /locus_tag="AC16G0_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101117.1"
                     /translation="MADQATCPIHGVPDCFKPALDAYVQQAYAGYCTKIDWPFVRIIP
                     KGWKPGVTLQELVAAKGVAASTTAKGTVAAAVGAAVVKAAKVEFYYSSAVEPAKNFFC
                     DNKKALHLCDQLKAKGVHVAVQDCAKQPVSFAVYNAAVTGPSAAKRAVFGAKGALEED
                     MGKAVPAVLVYAKADDKYPVEVFPRTDKDLGRLMGVEEALELLIKNA"
     gene            1759..2016
                     /locus_tag="AC16G0_15805"
     CDS             1759..2016
                     /locus_tag="AC16G0_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101118.1"
                     /translation="MLVASFFSGRVTVGVACATGMDAGGGAGFAAGGLNAVRALATDF
                     FFGAATRPLPRTGSAARAFLGLLPRAAPFAGAARRVFVLAL"
     gene            complement(2048..2458)
                     /locus_tag="AC16G0_15810"
     CDS             complement(2048..2458)
                     /locus_tag="AC16G0_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101119.1"
                     /translation="MADFKHCSLMLECSKCQMVNYLDPFTFWFFEGKIKCAGCDAIWA
                     LKIDNGQRVSGPTEAKPPHDKLPGYAQSKDYKTKITDTTKVNPPVMARADFVGKPIPI
                     FKSIRGKPVSGGPLKPEDLVGSRPRFIMEGKHYK"
     gene            complement(2517..>3384)
                     /locus_tag="AC16G0_15815"
     CDS             complement(2517..>3384)
                     /locus_tag="AC16G0_15815"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIR5101120.1"
                     /translation="GRVRASYDILGQAQGGCFSVTGFPAPGHGHPAIGLPSKHTIWLA
                     DYWGGMMGCFAGLAALWWRDNVSGLGELIEYSQVHGATRHLESALPLYGRYGVVKQRW
                     GNWDTEMCVHGIIQCGKSSYPTSKNPQEIEQGCILISAPEDDQFAKLCGVVSTLKPLA
                     GKYATTEARVQPAAQTEIYNALEAWAKDKTKEDVWKIMEDAGINSQPVWSNKEVVQQE
                     HFQMRDELHWLDDPYFGDVLSQGLPYHLSETPARHRWVFKPVGADNEYILQKYCGYGL
                     SEIRELERNEII"
BASE COUNT          536 a         1122 c         1027 g          699 t
ORIGIN      
        1 ggccggcccg atggagcccg gcttcacccg cttcgtcgcc gcgctggaga tgaatacgta
       61 catcatctcc gtggtgccca gcccctcgaa aatctctatc ccgaatttgc tcttccagtc
      121 gtagtacgtc tgggcggtca acgactcccc gccgccggtg cagatgcgta acgagctgag
      181 gttgtacttc ttctcggcgt ccggaacctg cagcatcttc cgatatgccg tgggaagaat
      241 cgagagcacc gtgaccttgt ggttctggat tgtctcgaac atggcctcgg gggtgaaccg
      301 ggcgatgagc gacacggccg ccccgaatcg gtacgggatg accgaaaccg tcgagtatcc
      361 ggccgccatg gcgatgggcg cagatccccc gatgacgtcg ttctcgctga cgttccagca
      421 gtgcttcccg aatccgtcgg gaacgatcag ggcttcctcc atgaagtggg ccgtcccctt
      481 gggcaggccg gtggttccgg aggtgaacag caggaccgag acatccatcc ggtcgcggcg
      541 gaccggatcg cactccggct tctctgcgtc tatgagctcc tgccacagga gatagccttt
      601 ctgcttccac gccccttgct gctccggcgt gcctccgacg acgatcacgt gctcgatcgt
      661 cttgcagttc ggccgtgcct tctccagctc ctccagcatg gctccggcca cgatgaccgc
      721 cttggcctcg gccaggttgg aaacgtgggc gatttccgcc tgggagaaca gggccgacgt
      781 cgggaggctc acgcccccga tcttgatggc ggcgaagttg gccatgaccg ccggcgggat
      841 gttggggagg cgcaacatca cccggtccgc ctccccgatc cccaggcgcc tcagggcgct
      901 ccccaacttg ttgacggttg ccaggaccgc tttgtagggg atcttcctgt cctcgaagtg
      961 gatggcaatc cgatccccct tccccgcagc cacctgcttg tccagcaatt cctcggtgct
     1021 gttgaagcgc tgcgggtagt gctgatactc ggggagcgtg tagatgcgtg tcggccgcag
     1081 ctccccggtc ggcaaatagt cggcaggaat tcgccccatg caccctcctc taattgtcag
     1141 gcgttcttga tcaatagctc cagagcctct tccacgccca tcagccggcc caggtccttg
     1201 tccgtgcgag ggaagacctc caccgggtac ttatcatcgg ctttggcata taccagcacg
     1261 gccgggaccg ccttccccat gtcttcctcc agggcgccct tggccccgaa gacggcgcgc
     1321 ttggcggcgc tgggcccggt cacggccgca ttgtaaaccg caaacgagac cggttgcttg
     1381 gcgcagtcct ggaccgcgac gtgaacccct ttcgccttga gctgatcgca caggtggaga
     1441 gccttcttgt tgtcacagaa gaaattcttg gcaggctcaa ccgcgctgct gtaatagaac
     1501 tcgaccttgg ccgccttgac caccgcggca ccgactgccg cggcgacggt tcccttggcg
     1561 gtagtcgacg cggcgactcc cttggccgcc acaagctcct ggagtgtcac gccgggcttc
     1621 caccccttgg ggatgatccg gacgaacggc cagtcgatct tggtgcaata gccggcgtac
     1681 gcctgctgaa cgtacgcgtc cagcgcaggt ttgaagcaat cgggaactcc atgaatcggg
     1741 cacgtggcct gatctgccat gctcgtcgcc tccttcttca gtggtcgagt tacggtcggg
     1801 gttgcctgcg cgaccgggat ggacgccggg ggaggcgctg gctttgccgc gggtggtttg
     1861 aatgcggtcc gggctttggc caccgacttc ttcttcgggg ccgccacccg ccccttgccg
     1921 cgcaccggtt cagccgcccg cgccttcttg ggtttgctcc cccgggcggc ccccttcgcc
     1981 ggggcagccc gccgagtctt tgtgctagcc ctctgacctt tcggcttcgg catttgacct
     2041 ctcccgtcta cttatagtgc ttcccctcca tgatgaagcg gggccgactg cccaccagat
     2101 cctctggctt gagtggtccg ccgctcaccg gtttgccccg gatgctcttg aagatcggga
     2161 tcggcttgcc gacgaagtcg gcccgggcca tgaccggagg attcaccttg gtggtatccg
     2221 taatcttggt cttgtaatcc ttcgactgcg cgtagcccgg cagcttgtcg tgcggcggct
     2281 tggcctcggt ggggccggag acgcgctggc cgttgtcgat cttgagcgcc cagatagcgt
     2341 cgcaacctgc gcactttatc ttcccctcga agaaccagaa ggtgaaggga tccaggtagt
     2401 tgaccatctg gcacttggaa cattccaaca tcagcgagca atgcttgaag tcagccatgc
     2461 agccaccccc ttctccgggt cgcaggggtc gcggtggcgt ccccgtcgga cacgcctcag
     2521 atgatctcgt tccgctccag ctcgcgaatc tcgctcaggc cgtagccgca atacttctgg
     2581 aggatgtact cgttgtctgc ccccaccggc ttgaagaccc accggtgccg ggccggcgtc
     2641 tcggagaggt ggtacggcag cccctggctc aggacatcgc cgaagtaggg gtcatccagc
     2701 cagtgcagct catcccgcat ctggaaatgc tcctgctgga ccacttcttt gttgctccag
     2761 accggctggc tattgatgcc ggcatcctcc atgatcttcc agacgtcttc cttggtcttg
     2821 tccttggccc aggcctccag cgcattgtag atttcggtct gggctgcagg ctggacgcgg
     2881 gcctcggtgg tggcgtactt gcccgccaga ggcttcaggg tcgaaaccac cccgcacagc
     2941 ttggcgaatt gatcatcctc cggagcagag atgaggatgc agccctgctc gatctcctgc
     3001 ggattcttcg aagttgggta ggacgacttc ccgcactgga tgatcccgtg gacgcacatc
     3061 tcggtatccc agttgcccca gcgctgcttg accaccccgt agcgcccata cagcggaagg
     3121 gcgctctcca ggtgccgcgt ggcgccgtgg acttggctgt actcgatgag ttctccgagg
     3181 cccgagacgt tatctcgcca ccacagggca gccagtccgg cgaagcagcc catcatgcct
     3241 ccccagtagt cggccagcca gatggtgtgt ttcgatggca acccgatggc ggggtggccg
     3301 tgacccggag ccgggaagcc cgtcaccgag aagcagccgc cctgggcctg gccgagaatg
     3361 tcgtacgagg cacggacccg gccc
//
LOCUS       DBNGZC010000916         2001 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1641727, whole genome shotgun sequence.
ACCESSION   DBNGZC010000916 DBNGZC010000000
VERSION     DBNGZC010000916.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1641727"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1190)
                     /locus_tag="AC16G0_15820"
     CDS             complement(156..1190)
                     /locus_tag="AC16G0_15820"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR5101121.1"
                     /translation="MKTQSKQSTRSQGSGHISRRRFMALAGATAALPLIHVQPARAAK
                     VTMKVAHVLTEADIIHRATAKFKELAEKKAGGELEVQIYPTSALGSLRVTWESMQLGN
                     LEGGNWDTVTPAMMVPQYAGTELPFVFRDLDHVHKVFDGPIGQGLEKALVDKAKVRTI
                     AVYDTTFRKIFTKTKAINSLADMKGLKIRVPEAQNYVRCMQLLGANPTPVAWGELYTA
                     LQMGVVEGFENKAEAAFNAKLHEQTRFMAYTGHIFCPNPFLCSERWFSKLSKNVQQAI
                     VEAGTDSLEWQRKEAPVSEKAFEQKLKDAGVTFTTPDPAPFRKAVEPFYKEWGDKYAG
                     NDLIKQIREA"
     gene            complement(1573..>2001)
                     /locus_tag="AC16G0_15825"
     CDS             complement(1573..>2001)
                     /locus_tag="AC16G0_15825"
                     /inference="COORDINATES: protein motif:HMM:NF013381.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HIR5101122.1"
                     /translation="SPRHFKEFILPGLKRCVDAAHEAGAYVVKHTDGNIMPILDMIVD
                     TGIDGLNPLEPVAGMDIGLIKQRYGDRIALVGNIDCGYLLSQAPVEEVRRVTRETIRV
                     AAPGGGYCLSSSNSIHSSVKPENFMAMIETWRECRAYPIS"
BASE COUNT          328 a          642 c          645 g          386 t
ORIGIN      
        1 gtcgtcacca ccaccatcgc gccgaagagg gccacggccg cccagttcac ggtcccttcg
       61 tagatacccc agacccgttg actcatgtgt cctacccgat acaacgatgc gggcggcacc
      121 gccgcccgca tcgtgtgtaa tgattccgcg ggagcttacg cctcgcggat ctgcttgatc
      181 aaatcattgc cggcgtactt gtcgccccac tccttgtaga agggttccac ggccttgcgg
      241 aagggagcgg gatccggggt ggtgaaggtc accccggcat ccttcagctt ctgctcgaac
      301 gccttctcgc tgaccggagc ctccttccgc tgccactcca gcgagtccgt gccggcttcc
      361 acgatggcct gctgcacgtt cttggacagc ttgctgaacc agcgctcgct gcacaggaag
      421 ggattgggac agaagatatg gcccgtgtag gccatgaacc tcgtctgctc gtggagcttg
      481 gcgttgaagg cggcctcggc cttgttctcg aagccctcca ccacccccat ctgcagggcc
      541 gtgtacagct ccccccaggc cacgggcgtc gggttcgcgc ccaggagctg catgcagcgg
      601 acgtagttct gggcttcggg cacccggatc ttcagcccct tcatgtcggc aaggctgttg
      661 atggccttgg tcttggtaaa gatcttccgg aaggtcgtgt cgtagaccgc gatggtccgc
      721 accttggcct tgtcgaccaa agccttctcc aggccctgtc caatgggtcc gtcgaagacc
      781 ttgtggacat ggtccaggtc gcggaacacg aagggcaact cggtgccggc gtactggggg
      841 accatcatgg ccggcgtcac cgtgtcccag ttcccgccct ccaggttccc gagctgcatg
      901 ctctcccagg tgacccgcaa gctccccagg gccgaggtcg ggtagatctg cacctccagc
      961 tcgccgccgg ccttcttctc ggctagctcc ttgaacttcg ccgtcgcccg gtggatgatg
     1021 tccgcctcgg tgaggacgtg ggcgaccttc atcgtgacct tggccgctcg cgccggctgc
     1081 acgtggatca gcggcagcgc ggcggtggca cccgccaggg ccatgaagcg gcggcgggat
     1141 atgtggcccg acccctggga tcgcgtggac tgtttcgatt gcgttttcat gattccccct
     1201 ccttgtgatc agtatgccgt gaacttggcc atggcgctgc aggacgccgc cgcgcaggaa
     1261 aactaccggg cgccgggctg gtcacgaccg gaaggatgga gctccgccag cggggtgtcg
     1321 gagcgaccgg tccgtgaaag cccggaaggc aggaatggat ggcaggcacc ctagcagcac
     1381 cccggatcgg tgtcaatcgg ggtgaaggtc acctgttcag tgttcaatac attttggagg
     1441 gcggcgagag agtcatgctt ccagtggccg cctctgcgcg ccagaggaaa agtgggctcg
     1501 atctctctga cactcacgtg gctgtggtga cgcccgaggc acggatacct aatcaggctc
     1561 gagaccggcg ggtcagctga tcggatacgc ccggcactca cgccacgtct cgatcatggc
     1621 catgaagttc tcgggcttga ccgaggagtg gatgctgttg gagctggaga ggcagtagcc
     1681 gccgcccgga gccgcgacgc ggatcgtctc ccgggtgacc cggcgaactt cctcgaccgg
     1741 ggcctggctg aggagatagc cgcagtcgat gttgccgacc agggcaatcc ggtcgccgta
     1801 gcgttgcttg atgagcccga tatccatccc cgcgacaggc tcgaggggat tcaggccgtc
     1861 gatcccggtg tccacgatca tgtcgaggat cggcatgatg ttgccgtcgg tgtgcttcac
     1921 cacataggcg ccggcctcgt gggccgcgtc cacgcaccgc ttgaggcccg gcaggatgaa
     1981 ctccttgaag tggcgcggcg a
//
LOCUS       DBNGZC010000917         6800 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1052885, whole genome shotgun sequence.
ACCESSION   DBNGZC010000917 DBNGZC010000000
VERSION     DBNGZC010000917.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6800)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1052885"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="AC16G0_15830"
     CDS             complement(<1..626)
                     /locus_tag="AC16G0_15830"
                     /inference="COORDINATES: protein motif:HMM:NF013383.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HIR5101123.1"
                     /translation="MATVTIVGAGLMGTATAYPLAENGHGIRLVGTHLDADIIQSCKE
                     RHFHPRLKRELPPHVRAFFLEEIAEAFVGTDIVVCGVNSLGIHWMAETIGPLLRPGQM
                     IIAITKGLEITAEGDLRILPDVLESELPAGVRGQVRLAAVGGPCIAGELAGRRQSCVI
                     FGSRDPATLDALGAAFRTSYYHVWTTTDLVGLEYCAALKNAYALGVGLA"
     gene            complement(798..1505)
                     /locus_tag="AC16G0_15835"
     CDS             complement(798..1505)
                     /locus_tag="AC16G0_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5101124.1"
                     /translation="MLEVHELNVYYGAIHALQGISFTLNEGEIVTLIGANGAGKTTTL
                     STISGLLRPRRGFVRFKDQDITMMPAEQIVRQGISQVPEGRKIFAPLTVQENLEMGAF
                     TRTDKAEIERSFQRVFASFPRLKERLHQLGGTLSGGEQQMLATARGMMSRPSLLLLDE
                     PSMGLSPIMVEEIFKIIQEINSQGTSILLVEQNALMALSVAHRAYVLETGRITLEGTA
                     KELRENPQVKAAYLGVH"
     gene            complement(1498..2304)
                     /locus_tag="AC16G0_15840"
     CDS             complement(1498..2304)
                     /locus_tag="AC16G0_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5101125.1"
                     /translation="MKGTEASAPSTPTLEIRGLTKYFGGLRAVSAFDLTVKSGELAGL
                     IGPNGAGKTTVFNMISGLYGPSGGEILFQGEGTIGMEPFEITQLGIGRTFQNIRLFPN
                     LTVLDNVRIAYHPHAGYGMFDAILRNKRFEARERELTERAQDFLAIFKLERIQNDLAK
                     NLPYGQQRRVEIARALASDPRLLLLDEPAAGMNPAEIVTLMELIHFIRDRFDLTILLI
                     EHQMRVVMGICEHITVMDFGEVIARGTPKEIQDNPKVIEAYLGKGAASAA"
     gene            complement(2320..3690)
                     /locus_tag="AC16G0_15845"
     CDS             complement(2320..3690)
                     /locus_tag="AC16G0_15845"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5101126.1"
                     /translation="MEINLGFLALSVIKIGVVMAWIYGLLWALEHESWSVKAVGTLLA
                     FAVIQAALSVQVGSAAIMNDFDAGLLFQACTMAMVALGLNLIYGFNGQFSLGQWGFYG
                     IGAYAAADVTYRWTNGDAKGLLVIGLGVVLGGLAIWCVNRMLLRYKGMPVLSAFTLYF
                     LGSILAWAVAVVAGSAASAALAPLFGTKGAPGLLASGPVLQVIFFLSVLLAGAFAAEV
                     SFLFGLPVLTLGSDYFGIATLGFTIVVNTLMVNSDTILPFPEMKGGRGMIGIPKLTTW
                     FWAFVFLLLIIVIMRNLIHSSTGRAIMSVREDETASKVMGIDVAYYKLLPFVIGSLFA
                     GIGGGVYAHFIGFLSPGTFDFLVGFNPLIIVVFGGLGSMSGSIAASFGWIFFLEGLLR
                     VILGQMGTEAPTWRFVLYPIALLLLMLVRPEGLLGTKEWGFFRAPRIRPRAVTEPQAA
                     AAPATK"
     gene            complement(3701..4768)
                     /locus_tag="AC16G0_15850"
     CDS             complement(3701..4768)
                     /locus_tag="AC16G0_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5101127.1"
                     /translation="MAWIGQGFLLLLAAVALFILVRDFIKDPALFIQIIISGLQLGFV
                     YALIALGYTMVYGIVKLINFAHGDVFMVGSFVSFYAVTRFQLHVWPAAFIPGLPHGWV
                     VVIGSLSVMLLSMIVCVAMAVTIERVAYKPLRNAPRIAALITAIGVSFFLEFFGALSF
                     VFSPRFIPYDRPFDVVTWYIKDGVYLMRPGMTLPDHSITFSNIFLIIVAASILVQLIL
                     QFLVRHTKIGKAMRAASFDKPTARLMGINVDRVISFTFAVGAAFAGLGGVLYAIAYSS
                     IWSQLGILPGLKAFVAAVLGGIGSIPGAFVGALIMGQAEALTMGYISTPMRDAVAFAI
                     LIIVLLVRPTGIFGEPEKEKA"
     gene            complement(4925..6076)
                     /locus_tag="AC16G0_15855"
     CDS             complement(4925..6076)
                     /locus_tag="AC16G0_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5101128.1"
                     /translation="MRAIKLVSLLLFTGIILAGCGGGGGDTIKVAILAPLSGPVPTFG
                     VMTRDGALLAIEEWNAKGGVLGKKIQAVVEDSQCTPDPAVNAANKVIDQDKVHYIVGE
                     VCSKASIPISEIANSKKVIEISPTSTNPDVTVGKDGKVKDFIFRACFIDPFQGTVGAK
                     FAIDNLKAKTAFIMLDQANDYVKGLAEFFEKAFTAGGGQIVGKETYTSKDTDFSAILA
                     KVAAAKPDLVYLPDYYNIVNLVTKQAKEKGIKAPFMGGDGWDSSDMDLKAADGGYFTN
                     HYSPQDTRPEVQGLVKAFGDKYKDDKSQPKVPDALAALAYDATNLLLQGIKEAGADDT
                     AKVKDALAKISFNAVSGNITFDANHNPVKSATILAIKDGKVAFNSVVNP"
     gene            6515..>6800
                     /locus_tag="AC16G0_15860"
     CDS             6515..>6800
                     /locus_tag="AC16G0_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101129.1"
                     /translation="MQHRAIATRGFIVGVLILQLVPLVLFPPESFSSNSQEWWLPLLL
                     AAMVLVACVQLILRGSNQLWPWHLMSFSQGFNIISRLMMLWPHATKTVGGA"
BASE COUNT         1366 a         2062 c         2157 g         1215 t
ORIGIN      
        1 gcaagcccca cacccagggc gtaggcgttc ttgagggcgg cgcagtattc taagcccacc
       61 aggtcggtgg ttgtccagac atgatagtag gatgtccgga aagcagcgcc aagcgcgtcg
      121 agcgtcgccg gatcgcggga accaaaaatg acgcaactct ggcggcggcc ggccagctcc
      181 ccggcgatgc agggcccgcc gacggcggca agccggactt ggccccggac gcccgcgggg
      241 agttcactct ccaggacgtc tggcaggatc ctgagatcac cttccgccgt gatctccaga
      301 cccttggtga tggcgatgat catctgaccg ggccggagca gcggcccgat ggtctccgcc
      361 atccagtgaa ttcccagcga gttcacgcca cagacgacga tatccgtccc cacgaatgcc
      421 tcggcgatct cctcaagaaa gaaggcgcgg acgtggggtg gaagctcgcg cttgagccgc
      481 gggtggaagt gtcgctcctt gcagctttgg atgatgtcgg catccaggtg cgttcccacc
      541 agccggatac cgtgaccgtt ctccgccagc ggataggccg tggcggttcc catgagcccg
      601 gctcccacga tcgtgacggt ggccatggcg tgctcccctg tatgattctc gcgtgcgtcg
      661 gcgtccgccg gcgccctatt cgtactcgtg cgggacgatg gcggcgggtg ggtggaggag
      721 gctggctcac aggaggagag cgagggggaa aatcgatgaa gggttggaac cagtcgttct
      781 gggaagcaca acccgtccta atggacaccc aggtaggcgg cctttacctg tgggttctcg
      841 cgcagctcct tggccgtacc ctccagggtg atgcggcccg tttccagcac gtaggcgcgg
      901 tgggcgaccg acaaggccat cagcgcgttc tgctcgacca gcaggatgct cgtgccttga
      961 gagttgatct cctgaatgat cttgaagatc tcctccacca tgatggggct gaggcccatg
     1021 gacggctcgt ccagcaggag gaggctgggg cggctcatca ttccgcgggc ggtggccagc
     1081 atctgctgct caccgccgga gagcgttccg ccgagctggt gaaggcgctc cttcaggcgg
     1141 ggaaagctgg cgaacacccg ctggaaagag cgctcgattt cggccttgtc cgtgcgggtg
     1201 aaggcaccca tctccaggtt ttcctggacc gtgagcggcg caaagatctt ccggccctcg
     1261 ggcacctggc tgatgccctg acgcacaatc tgctcggccg gcatcatggt gatgtcctgg
     1321 tctttgaaac ggacgaaacc gcggcggggg cgcagcaggc cggagatggt ggacagggtg
     1381 gtggtcttgc cggcgccgtt ggctccgatg agcgtgacaa tctcgccctc gttgagggtg
     1441 aaggagatac cctggagcgc gtgaatcgcc ccgtaataga cgttgagttc gtggacttca
     1501 agcagcactg gcggctccct tgcccagata agcctcgatc accttggggt tgtcctggat
     1561 ctccttgggt gtgccgcggg cgatcacctc gccgaagtcc atcaccgtga tgtgctcgca
     1621 gatgcccatc acgacgcgca tctgatgctc gatcaggagg atcgtcagat cgaagcggtc
     1681 ccggatgaaa tggatgagct ccatcagggt gacgatctcg gcgggattca tccccgcggc
     1741 cggttcgtcc aggaggagca ggcgtggatc ggaggcgagg gcgcgggcga tctccacccg
     1801 gcgctgctgg ccgtagggga gattctttgc caagtcgttc tgaatccgct ccagcttgaa
     1861 gatcgccagg aaatcctggg cgcgctcggt cagctcgcgc tccctggcct cgaaccgctt
     1921 attgcgcagg atggcgtcga acatcccata gccggcgtgg gggtggtagg cgatgcggac
     1981 gttatccagc accgtcaagt tggggaagag ccggatattc tgaaatgtcc gcccgatgcc
     2041 caactgggtg atctcaaagg gctccatccc gatggtgccc tctccctgga acagaatctc
     2101 gccgccagag gggccataca ggccgctgat catgttgaat acggtcgtct tgccggcgcc
     2161 gttgggaccg atgagtcccg ccagctctcc gctcttgacg gtcaggtcga aggcgctgac
     2221 cgcccggagt cccccgaaat acttggtcag gccccggatc tccagcgtgg gggtcgaggg
     2281 ggcagaggcc tcggttcctt tcatgatcct gttccgtgac tacttggttg cgggcgccgc
     2341 ggcggcctgg ggttcagtca cagcgcgcgg ccggatccgg ggcgcgcgga agaaccccca
     2401 ctccttggtt ccgagcagcc cctcgggtcg gaccagcatc aggaggagca gggcgatggg
     2461 atagagcacg aatcgccagg tgggcgcctc ggtccccatc tgaccgagaa tgacacgcag
     2521 caagccttcc aggaaaaaga tccagccgaa ggacgcggcg atcgagccgc tcatgctccc
     2581 gaggccgccg aagacgacga tgatcagagg gttgaacccg accaggaagt cgaaggtccc
     2641 gggactgaga aagccgatga agtgggcgta cacgcccccg ccaatgccgg cgaacaggct
     2701 gccgatgaca aaggggagca gtttgtagta ggccacgtcg atgcccatga cctttgaggc
     2761 ggtctcgtcc tcccggacgg acatgatggc gcggccggtg ctggagtgga tcaggttgcg
     2821 catgatcacg atgatcaaca gcaggaacac gaaggcccag aaccatgtcg tcagcttggg
     2881 gatgccgatc atgccccggc cgcccttcat ctccgggaag ggcaggatcg tgtccgagtt
     2941 caccatcagc gtattgacga caatggtgaa gcccaaggtg gcgatgccga agtagtcgct
     3001 gcccagggtc aggacgggga ggccgaacag gaagctaacc tcagccgcga aggctccggc
     3061 cagaaggacg gagaggaaga aaatcacctg aagaaccgga ccgctggcga gcagccctgg
     3121 tgcccccttg gtcccgaaga gaggggcgag ggcggcgctc gccgcactgc ccgccacgac
     3181 cgccacagcc cacgccagga tgctgcccag gaaatacaac gtgaatgccg agaggaccgg
     3241 cattcctttg tagcggagca gcatccgatt gacacaccag atcgccagtc cgcccaggac
     3301 cacgcccagg ccgatgacga ggagaccctt cgcatccccg tttgtccaac ggtaggtgac
     3361 gtccgcggcc gcgtaggcac cgataccgta aaagccccac tgccccaggc tgaactgccc
     3421 gttgaaaccg tagatcagat tcagacccag cgcgaccatg gccatggtgc aggcctgaaa
     3481 cagcaggccc gcatcgaagt cattcatgat cgctgcgctg ccgacctgga cagacagggc
     3541 tgcctggatg accgcgaagg ccagaagggt gcccaccgcc ttgacggacc aggactcgtg
     3601 ctccagcgcc cacaggagtc cgtagatcca ggccatgacc acgccgattt tgatgacaga
     3661 taatgccaga aagcctaggt tgatctccat cggtggtccc ttatgccttt tccttctccg
     3721 gctcgccgaa gatccccgtc ggccgcacca ggagaacgat gatcaggatc gcgaaggcca
     3781 cggcgtcgcg catgggcgtg gagatatacc ccatggtcag cgcctcggct tgccccatga
     3841 tcagggcgcc gacgaaggcg ccggggatgc tgccgatccc cccaagtact gccgccacga
     3901 acgccttcag accgggcagg atacccaact gggaccagat ggaggagtag gcgatggcat
     3961 acaggacgcc gcccaatccc gcgaaggccg cgcccacggc aaaggtgaag gagatgaccc
     4021 ggtccacatt gatgcccatc agccgcgccg tcggcttatc gaaggaggcg gcgcgcatgg
     4081 cctttccaat cttggtatgc cgaaccagga attgcaaaat cagctgcacc agaatcgagg
     4141 cagccacgat gatcaggaag atgttcgaaa aggtgatgct atgatcaggc agggtcatgc
     4201 cggggcgcat cagatagacg ccgtccttga tataccaggt cacaacatca aaagggcggt
     4261 catatgggat gaagcgaggc gagaacacaa agctcagggc gccaaagaac tccaggaaga
     4321 aggatacccc aatggccgtg atcagggcgg cgatgcgggg cgcgttgcgt aacggtttgt
     4381 aggcaacccg ctcgatggtg acggccatgg ccacgcacac gatcatggaa agcagcatca
     4441 cactcagcga gccgatgacg accacccagc cgtgaggcag gccggggatg aaggccgccg
     4501 gccagacgtg aagctgaaag cgagtcaccg cgtagaagct gacgaaggag cccaccatga
     4561 acacatcccc gtgggcgaag ttgatcaatt tgacgatgcc atacaccatc gtgtagccca
     4621 gtgcaatgag ggcgtacacg aagccgagct gcaggccgct gatgatgatc tggatgaaga
     4681 gcgccggatc tttgatgaaa tcgcggacca ggatgaagag ggccaccgcg gccagaagca
     4741 ggaggaaccc ctgtccgatc caggccatgc gggcgccggg tctgcgcgct gcaagagagg
     4801 acatcggggg aagtatcacc tgtggcatca atgcctgacc tcgtgacaga ggcgaggggg
     4861 actgaacgca tggagggcga ccggggtcgc cctccatgac cagtgatcga cgctgcgacg
     4921 agccttacgg attcaccacg gagttgaaag ccaccttccc gtccttgatc gccaggatgg
     4981 tggccgactt gaccgggttg tggttggcat cgaaggtgat attacccgac accgcattga
     5041 agctgatctt ggccagggca tccttcacct tggcagtgtc atccgcgccg gcctccttga
     5101 tgccctgcag cagcaggttg gtcgcgtcgt aggccaaggc ggccagggcg tccgggacct
     5161 tcggctggct cttgtcatcc ttgtacttgt cgccgaaagc cttgacaagg ccctggacct
     5221 ccgggcgcgt gtcctgtggc gagtagtggt tggtgaaata gccgccatcg gcagctttga
     5281 gatccatgtc ggacgagtcc caaccatccc cgcccatgaa cggggccttg atgcccttct
     5341 ccttcgcctg cttggtcacc aggttgacga tgttgtagta atccggcagg tacaccaaat
     5401 ccggcttggc tgccgcgacc ttcgccaaga tggccgagaa gtcggtgtcc ttgctggtgt
     5461 aagtttcctt cccgacgatc tggccgccgc cggccgtgaa ggccttctcg aagaactccg
     5521 ccaacccctt gacgtagtcg ttggcctggt caagcatgat gaaggccgtc ttcgccttga
     5581 ggttgtcgat ggcgaacttg gcccccacag tgccttggaa cgggtcgatg aagcaggcgc
     5641 ggaagatgaa gtccttgacc ttgccgtcct tgcccacggt cacgtccggg ttggtcgagg
     5701 tcgggctgat ctcgatgacc ttcttgctgt tcgcaatctc ggaaatcggg atggatgcct
     5761 tggagcagac ctcgccgacg atgtagtgga ccttgtcctg atcgatgacc ttgttggcgg
     5821 cgttcaccgc cgggtcaggg gtgcactggc tgtcctccac cacggcctga atcttcttgc
     5881 ccaggacgcc acccttggcg ttccattcct cgatcgccaa gagcgccccg tcgcgggtca
     5941 tgacgccaaa ggtgggcacc ggaccggaca gcggggccaa gattgcaacc ttgatcgtgt
     6001 caccaccgcc accaccacag ccagccagga tgatccccgt gaacaacagc agacttacca
     6061 acttgatcgc acgcatgggt gattctcctc caaagaatgg ttttgaccca ggctctactg
     6121 cgccgctaga ctgcagcaat cagccaaacg cgggaggccc gatcccttgt ttagcctgtt
     6181 ccgccggtgg tggtgggcgc aaccccctcc tttcgggtgg aatgcggcga ggcacaccac
     6241 accacgccac gctagctgac gaattgcaca agtgaatggc ggataatcct cggccggctc
     6301 tgcaaaggag cccaaagggt cgtcacgtag agtcggtccg gcgagctaag gtacttctat
     6361 atccaagccg ccaataaatg tcaagcgact tgacaggaac gcacgggcga cctataagga
     6421 tgcaaacaaa gggttttggc gcacctcggg aaagccgacg ctttgagttc gaggcctggt
     6481 gcccggttcg tcagacaagg agagatagca tatcatgcaa caccgagcaa ttgcgacacg
     6541 cgggtttatc gtcggggtcc tcatcctcca actcgttccg ctggttctgt ttcccccgga
     6601 gtccttctcc tcaaactccc aggaatggtg gctcccgctc ctgctggctg ccatggtgct
     6661 ggtagcctgc gtccagctca tcctgcgcgg cagcaatcag ctctggccat ggcacctcat
     6721 gtccttctct cagggtttca acatcatcag ccgcctgatg atgctttggc cgcacgcgac
     6781 gaagacggtt ggtggagcca
//
LOCUS       DBNGZC010000918         1986 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2719403, whole genome shotgun sequence.
ACCESSION   DBNGZC010000918 DBNGZC010000000
VERSION     DBNGZC010000918.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2719403"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..94
                     /locus_tag="AC16G0_15865"
     CDS             <1..94
                     /locus_tag="AC16G0_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HIR5101130.1"
                     /translation="WPEDRNTTHWLENRGTIPAVEISVDIVRQE"
     gene            290..562
                     /locus_tag="AC16G0_15870"
     CDS             290..562
                     /locus_tag="AC16G0_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101131.1"
                     /translation="MVEEEPYFLEMVRHLHLNPLRAGVVKDLAALDRYPCSGHAALRG
                     WLPRPRQDIETVLTQFAPRRRDARRHYQAFVAAGVAQGRRPSGQRL"
     gene            complement(664..1839)
                     /locus_tag="AC16G0_15875"
     CDS             complement(664..1839)
                     /locus_tag="AC16G0_15875"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5101132.1"
                     /translation="MWEIDAVEARTLLMDLVAIDSVNPTLVPGGAGEERVARRLAAYA
                     QSLGFAARLDYVTPTRPNVVAVCGGPTAGEAALDQRHGLLLNGHTDVVDVQGMTDPFT
                     PRVEGDRIYGRGAGDMKGGLVAALLAMKAVRDAGVRLKKSVLFTGVVDEEYASLGTEA
                     VARRYDAQAALVLDGAMAPEIAHKGFAWVTVETVGKARHGSRYLEGIDAIAMMGRFLA
                     RMDALNAKYQKEAPHPLCGHKSIHASLVEGGTALSTYPGQCTARLERRTLPREDPRLI
                     VEELAAITRDLQAQDERFSATLRLDFVRDGYEIDPSHPLVGTLTQAMLRQMGGQPTLV
                     GAGGWKDSAILGAKGIPTVIFGAGGQGAHSLEEWAEMGNVLTCARVLADVIVALCGE"
BASE COUNT          330 a          667 c          635 g          354 t
ORIGIN      
        1 ctggcccgag gataggaaca ccacacactg gcttgagaac agaggaacga ttccggcggt
       61 ggagatctcg gtcgatatag tcaggcaaga gtaagctcag gcccgacatc taacacggtc
      121 ggacctaaca cgcggctcaa ccggatgcgc ttcagtcggc ttcgccacct ttcgcgcgcc
      181 cattagccac aacgttagtt ggtcacttcg tcgttcctgt tgccccttcg cccgccgaac
      241 gtggccttta taacggtcgg gtgttgccct gaattgctgc aagtccatcg tggtggagga
      301 ggagccgtac ttcctggaaa tggtgcgcca tctccatctg aatccgctgc gggcgggtgt
      361 ggtgaaagac ctcgccgcct tggatcggta tccctgcagt ggccacgcag ccctccgggg
      421 atggttgccg cgtccccggc aggacatcga aaccgtcctc acccagttcg caccgaggag
      481 aagggacgcc cgccgccact atcaggcctt tgtggcggct ggcgttgccc aaggccggcg
      541 accgtcaggc cagcggcttt gagggtggtg tccggggaac aatctctaaa ctactcgcgc
      601 aatgtccccc atttcgtctg ccatttgatg ggtgacgatg cgagtgatgg ggggacgata
      661 tcctcactcc ccgcagagtg cgacgatcac atccgccagc actcgggcgc aggtgaggac
      721 attgcccatc tccgcccatt cctccaggct atgcgcgccc tgcccgccgg cgccgaagat
      781 gaccgtgggg atgcccttgg cccccaggat ggcggagtcc ttccagccgc cggcccccac
      841 aagggtgggc tggccgccca tttgccgcag catggcctgc gtgagggtcc cgaccagggg
      901 gtgcgaggga tcgatctcgt agccgtcccg cacaaaatcc agcctgaggg tggccgagaa
      961 gcgctcgtcc tgggcctgca agtcgcgcgt aatggccgcg agctcctcca cgatcaatcg
     1021 cggatcttcg cggggcagcg tccgccgttc gagccgggcc gtacactgac cggggtaggt
     1081 gctgagcgcg gtgcccccct cgaccaagga ggcatggatg gacttgtgcc cgcatagcgg
     1141 gtgcggggcc tccttctggt acttcgcgtt gagcgcgtcc atccgggcga gaaagcgacc
     1201 catcatggcg atggcgtcga tcccttcgag gtaccgggag ccgtggcgcg ccttgccgac
     1261 tgtttcgacc gtcacccagg cgaacccttt gtgggcgatc tccggcgcca tggcgccatc
     1321 gaggacgagc gcggcctggg cgtcgtagcg ccgggccacc gcctcggtcc cgagactggc
     1381 gtactcctcg tccacgaccc ccgtgaacag gaccgacttc ttcagccgga cgccggcatc
     1441 ccggacggct ttcatggcca ggagggcggc caccagcccg cccttcatat ccccggcgcc
     1501 gcgcccgtag atgcggtcac cctccacccg cggcgtgaag ggatcggtca ttccttgcac
     1561 atcgaccaca tccgtatggc cattcaggag caggccatgc cgttggtcga gggccgcctc
     1621 gccggccgtc ggtccgccac agaccgcgac gacgttgggc cgggtgggcg tgacgtagtc
     1681 cagtcgggct gcaaagccga gactctgtgc gtacgcggcg agccgccggg caactcgttc
     1741 ctctcccgcg ccgcccggca ccagtgttgg attgactgaa tcgatggcga cgaggtccat
     1801 gaggagggtc ctggcttcta ccgcgtcaat ctcccacatg gcggcatcct cccgaggccc
     1861 agcttgggcc gccggcatat tcgcacaggc cagcccggaa tgccaggcta gacagatcac
     1921 gggcgtgaat ggtagcctcc tgaccttcgc ggaggtactc atgggacgaa ccgttgcccc
     1981 tttcag
//
LOCUS       DBNGZC010000919         1738 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_469387, whole genome shotgun sequence.
ACCESSION   DBNGZC010000919 DBNGZC010000000
VERSION     DBNGZC010000919.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_469387"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="AC16G0_15880"
     CDS             complement(<1..630)
                     /locus_tag="AC16G0_15880"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0070085 - obsolete glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5101133.1"
                     /translation="MGYLAPLFSPFLVAFGAALIFTPICRLIAVRFSLVDQPGGRKIH
                     GNPTPLLGGVAIASAQVVGVLASGSVDRVFISIPVAAGLMLLLGVYDDLNPQGGGVRW
                     WLRLIFQGILAVFVVHTGIRISFLQSPWLSIPITLLWIVGITNAFNLLDNMNGLSAGV
                     AAIAGLTFAALAARYMDVGPEQFPTAMAGAALAGACLGFLPSNFRGRIFM"
     gene            complement(634..>1738)
                     /locus_tag="AC16G0_15885"
     CDS             complement(634..>1738)
                     /locus_tag="AC16G0_15885"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR5101134.1"
                     /translation="ADYADCAQADAVVICVPTPFTRMKAPDLSFIEAAATALAPHVAS
                     GALVVLQSTTYPGTTAELVRPILEAPGRRAGTDFLLAFSPERIDPGNKQYSAHNTPKV
                     VGGIDPASTAAAADLFGLLVPADQIQRVSGPEVAEMCKLLENIFRSVNIALVNELTKL
                     CDRMGIDIWEVIDAAATKPFGFMPFYPGPGVGGHCIPVDPYYLSWKAREYDFYTKFIE
                     LAAEVNSSMPFYTVNKLAEALNRHGKPVQGAKVLVLGAAFKKDIDDPRNSPALEVMRA
                     LRARGAEVSYHYPYVPTVSLDGEHSAGTASPARLASVPLSEAVLAAQDCVCLAVAHTA
                     FEVPWLIKHSRLLLDATGVTRRFPQERGTVIRL"
BASE COUNT          309 a          547 c          625 g          257 t
ORIGIN      
        1 catgaagatg cgtccacgaa agttgctcgg gaggaatccg aggcacgccc cggccaaagc
       61 agcccccgcc atcgccgtag ggaactgttc cgggccgaca tccatatagc gcgccgccag
      121 ggcggcaaaa gtcaggccgg cgatggcggc cacgcccgcc gacaggccgt tcatgttgtc
      181 caggaggttg aaggcattgg tgatccccac gatccagagc agcgtgatcg ggatgctgag
      241 ccagggcgat tgcaggaagc tgatccggat ccccgtgtgg acgacgaaga cggcgaggat
      301 tccctggaag atcagtcgga gccaccagcg caccccgccg ccctgcggat tcaggtcgtc
      361 gtagacaccc aggagcagca tgagtcccgc agccacgggt atgctgatga aaacccggtc
      421 caccgatcca ctcgccagca ccccgaccac ctgcgcactg gcgatggcga ctccgcccaa
      481 gagaggagtc gggttgccat ggatcttccg gccacccggc tgatccacca ggctgaagcg
      541 cacggcgatc aggcggcaga tcggggtgaa gatcagggcc gccccgaagg ccaccaggaa
      601 cgggctgaaa agaggcgcca gatatcccat ggcctacaag cggatcaccg tcccgcgttc
      661 ctgggggaag cggcgcgtga cacccgtggc atccaagagg agccggctat gcttgatgag
      721 ccaggggacc tcgaaggccg tgtgtgccac ggccaggcag acgcagtcct gggccgccag
      781 gacggcctcg ctgagcggca ccgaggcgag gcgggcgggc gaggcggtcc cggcggagtg
      841 ctccccgtcc aaggagaccg ttggcacgta cgggtagtgg tagctcacct cggccccccg
      901 cgcccggagg gcccgcatga cctccagtgc cgggctattc cgggggtcgt cgatgtcctt
      961 cttgaaggcc gcccccaaga cgaggacctt ggccccctgg acgggtttcc cgtgacggtt
     1021 cagggcctcg gcgagcttgt tcaccgtgta gaacggcata ctgctgttca cctcggcggc
     1081 cagctcaatg aacttggtgt aaaagtcgta ctcccgggcc ttccaggaga ggtagtaggg
     1141 gtccacgggg atgcagtggc cccccacgcc ggggccgggg tagaacggca tgaagccgaa
     1201 gggcttcgtg gcggcggcgt cgatgacctc ccagatgtcg atccccatgc ggtcgcagag
     1261 cttggtgagt tcgttcacca gggcgatgtt tacgctgcgg aagatgtttt ccagcagctt
     1321 gcacatctcc gccacctcgg gtccagagac acgctggatc tggtcggccg gcacgaggag
     1381 gccgaacagg tcggccgcgg ccgcggtgga ggccgggtcg atccccccga ccaccttggg
     1441 ggtgttgtgg gccgagtact gcttgttccc cggatcgatc cgctcggggg agaacgcgag
     1501 gaggaagtcg gtccccgccc gccggccggg ggcctccaga atggggcgga ccagctccgc
     1561 cgtggtcccg gggtaggtgg tggattggag gactacaagg gcgccggagg cgacgtgggg
     1621 ggccagcgcg gtggcggccg cctcgatgaa ggagaggtcc ggggccttca tgcgcgtgaa
     1681 gggggtgggc acgcagatca cgacggcgtc ggcctgcgcg cagtcggcgt agtccgcg
//
LOCUS       DBNGZC010000920         2766 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_177179, whole genome shotgun sequence.
ACCESSION   DBNGZC010000920 DBNGZC010000000
VERSION     DBNGZC010000920.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_177179"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="AC16G0_15890"
     CDS             <1..186
                     /locus_tag="AC16G0_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101135.1"
                     /translation="ENPAWGYRRVHGELARLGHHLSAATVRRILRRDHRPAPRHQDTS
                     WRAFLRTQAEGLLACDC"
     gene            complement(363..2156)
                     /locus_tag="AC16G0_15895"
     CDS             complement(363..2156)
                     /locus_tag="AC16G0_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013871683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
                     /protein_id="HIR5101136.1"
                     /translation="MRDAATVLEIIRERGRRGLPLERVYRCLFNPDLYLLAYGKIYRN
                     AGAMTPGTTSETVDGMNLGKIEVIIGALRQERYRWTPVRRTYIEKKGTAKKRPLGLPT
                     WSDKLLQEVIRLLLEAYYEPQFSGRSHGFRPGRGCHTALQEIYHQWHGTVWFIEGDIT
                     DCFGSLDHSITRSILAGKIRDGRFLRLIDGLLQAGYLEDWRYHQTLSGCPQGGVVSPV
                     LSNIYLDRLDKFIEQTLLPAHNRGARRTPYRPCMRLWQRAFRLEQQGERKAGRALRKQ
                     MKTMPSRDPDDPGYRRLRYCRYADDWLLGFTGPRQEAEQIKACIGQFLHDQLKLELSP
                     AKTLITHGRTQAARFLGYEIVVLHADHKHDRRGHRSINAAIGLKVPVDVIRAKCAPYL
                     HHGRPIRRTERIVNTDFSIVAQFQAEFRGVAEYYRLAFNRHRLGQLKYVMERSLTKTL
                     ARKYRITVPKVYRRYRAVLDTGHGPRPGLQVTLDRDGGRPPLLAQWGGISLARDITPR
                     PLNDNPPRIWNSRRSELVQRLLAEACELCGSGNQVEVHHVRALKDLNPKGKKHQPEWV
                     TRMASRRRKTLVVCRVCHEDIHAGRPARRPR"
BASE COUNT          401 a          935 c          887 g          543 t
ORIGIN      
        1 gagaacccgg cctggggcta ccgcagagtg cacggggaac tcgccaggct tggtcaccac
       61 ctcagtgcgg cgaccgtgcg gcggatcctg cgccgcgatc accggccggc gccccgccat
      121 caggacacct cctggcgggc gttcctgcgc acccaagctg agggcctgct ggcctgcgac
      181 tgttgagtcg gcctggggcg cggtgccggg attgctcccg gtccgtttcc ccaggccgct
      241 cgccgaaccc gccgtgcggt agggtaggcc gccgacgagg tgccagttga cctggtcctt
      301 ttccgacggc ccccctccga accagccgtg cccgctttcc gtagcagctg gctctccggt
      361 gctcatcgtg gtctgcgtgc tggacgtccg gcgtggatgt cctcgtggca gacgcggcag
      421 acgacgaggg tcttgcggcg gcgggaagcc atccgcgtga cccattcggg ttggtgtttc
      481 ttccccttgg ggttgagatc tttgagggca cgaacgtgat gcacctcgac ctggttccct
      541 gatccgcata gttcgcaagc ctcggccagg agccgttgca ccagctccga ccggcggctg
      601 ttccagatgc gtggcggatt gtcgttgagg ggccgtggtg tgatgtcccg cgccaggctg
      661 atgccgcccc attgcgccag cagcggcggc cttccgccat cgcggtccag cgttacctgc
      721 aatccagggc gcggaccgtg cccggtgtcg agtacggcgc ggtagcggcg gtagaccttc
      781 gggaccgtga tccggtactt gcgggccagg gtcttggtca gcgaccgctc catgacgtac
      841 ttcagctgtc cgagccggtg acggttgaac gccagccggt agtactccgc gacaccccgg
      901 aattcggcct ggaactgcgc gacgatactg aaatcagtat tgacgatgcg ttcggttcgc
      961 cggatcggcc tgccgtggtg caggtagggc gcgcatttcg cgcggatcac atcgactggc
     1021 actttcagcc cgatcgccgc gttgatgctg cggtgaccgc gccgatcgtg cttgtggtcg
     1081 gcgtgcaaca ccacaatttc atagccgagg aaccgggcgg cctgcgtccg cccgtgggtg
     1141 atcagggttt tggccgggga cagttccagt ttgagctggt cgtgcaggaa ctgcccgatg
     1201 caagccttga tctgctcggc ttcctgccgt gggccggtga agcccagcag ccagtcgtcc
     1261 gcgtaacggc agtagcgcag ccgccggtag cccggatcat ccgggtcgcg ggatggcatg
     1321 gtcttcatct gcttgcgcag tgcgcgacct gccttccgtt ctccttgttg ttccagcctg
     1381 aaggcgcgct gccacagccg catgcatggc cggtatggcg tgcgtcgtgc tccccggtta
     1441 tgggcaggca gcagggtctg ctcgataaac ttgtccagcc ggtccaggta gatattggaa
     1501 aggacgggtg ataccactcc gccttgcgga cagccgctca gcgtctggtg gtagcgccag
     1561 tcctccaggt accctgcctg gagtaatcca tcgatcaggc gcaggaaccg gccatcgcgg
     1621 attttcccgg ccaggatcga tctggtgatc gaatggtcca aggacccgaa acagtcagtg
     1681 atgtcgccct cgatgaacca caccgtccca tgccactggt gatagatctc ctgcagggcg
     1741 gtatggcacc cgcggcctgg gcggaaaccg tgggagcggc cggagaactg cggctcgtag
     1801 tacgcctcta acagcaggcg gatcacctct tgcagcagct tgtccgacca cgtcggcaac
     1861 cccagcggac gcttcttcgc cgttcccttc ttctcgatgt aggtgcgtcg caccggagtc
     1921 caccggtatc gctcctggcg gagcgcaccg atgatcacct cgatcttgcc gaggttcatg
     1981 ccgtccacgg tctcgctcgt ggtcccgggg gtcattgcgc ccgcgttccg gtagatcttc
     2041 ccgtaggcga gcagatacag gtccgggttg aacaagcacc ggtagacccg ttccagcggc
     2101 aaccctctcc tgccgcgttc acgaatgatc tccaatacgg ttgcggcgtc tcgcatctcg
     2161 cgtacctcac cgtccttgcc gatctgagca cctgggtccc ttcgcccatg tagacggctc
     2221 tcccgtcctc cctggcaggg cgtgactccc gcgactacta cggaccctcc gtgaccatag
     2281 ggctcgcgcc ccgtaggcca tcccgaattc ccctgtgcgc cagacgtatc gagcgtgacg
     2341 taggcgtccc ggtccgtccc cttgaacggg ctcgctgccc gccgcccacc ggccgggagg
     2401 gttgagacga cgaaagtcgg aatcgcccta tgccgatggc cccggcaaca ggcgccgtgc
     2461 cggggggtgt gacgtttgca ccgctggaga ctgggtttca agcagtctgg ccttcgccgt
     2521 atcacgcggg ccttgcacgg cagcaccttc agcgccttca ctgcgctccg cgctttcccg
     2581 ccatgctctt gtcccctccc ggtttcccgt tcaggtaagg cgggtggccc agaggtcacc
     2641 tcctcccgaa cctctgcccc ctgcgggggc gatctggcgc cgggttcgac ggcgcacact
     2701 ctcatcgcac cgggcgctcc acgcgtcttg ccctgtcgtc agtcgcttgg cgcggccggc
     2761 tgtccc
//
LOCUS       DBNGZC010000921         1967 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_375699, whole genome shotgun sequence.
ACCESSION   DBNGZC010000921 DBNGZC010000000
VERSION     DBNGZC010000921.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_375699"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..1492
                     /locus_tag="AC16G0_15900"
     CDS             182..1492
                     /locus_tag="AC16G0_15900"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5101137.1"
                     /translation="MLQCVMGLGVVCLAAVALAGCETKASPAVGRPPAPVSIAEAVAQ
                     DVPVYLDEVGKTVAREVVTVQPQVSGPITAIHFTDGSDLKAGNPLITIDQRPFEASLR
                     QAQANLDRDRAQLGQVESALAQSLAAEKQAEANLARDMAQLQNAQAQERRYKGLIDDG
                     AVSREQYDQVRTAALAAEATVQADQAAINNAKAAIGAAQATVENAKAAIQADQAVAEN
                     ARIQLGYTVIRSPIDGRAGQRLVDPGNVVTANSTALLTIQRLDPIYADFTVTENDLTA
                     VQRNMAQGTLRVEVRLPDEPDTPRTGQLTFLDNAVQDATGTVKLRATIPNDDHRFWPG
                     RFVKIRLVLRTLQGAVLIPADAPQMSAKGPFVYVVKADATAELRPVVLGQRQGDRIVI
                     EQGLKPGERVVMTGQLGVTPGGKVRLQEPGVGGSPPAAGTGGKS"
     gene            1489..>1967
                     /locus_tag="AC16G0_15905"
     CDS             1489..>1967
                     /locus_tag="AC16G0_15905"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5101138.1"
                     /translation="MNLSELFIRRPVMTAVLTLSVILFGVLSYLRLPVNDLPAVDYPV
                     IQVQADYPGASPDTVANNIATPLERQFMQINGLELVTSKSTQGHASLTLQFALDKSID
                     AAATDVQTAISQATGSLPVDLPSPPTYSKTTPNDQPIRYIALPSDSVTPGQLYDYAST
                     "
BASE COUNT          366 a          698 c          623 g          280 t
ORIGIN      
        1 cgggcacctt gacgattcac gcggctacca accgcaatcc ggcgaaccgg cgccgcggcg
       61 cgtggatgtt ccgtgccagg cccgcccgga atgtccttga gtacaagaat caccgacccg
      121 atgcggatgc atcagggaca gaggtaacgg catgagaatc ttccccctcg atcagcgatc
      181 catgctccaa tgcgtgatgg gactcggggt cgtctgcctc gcggccgtcg ccctggccgg
      241 ctgtgagacg aaggcctcgc ctgcggtcgg acggccgccg gcgcccgtct ccatcgcgga
      301 ggcggtcgcg caagatgtcc ccgtatacct ggatgaggtc ggcaagaccg tcgcgcgcga
      361 ggtcgtcacg gtccagccgc aggtctccgg gccaatcacc gcgatccact tcaccgacgg
      421 gtccgaccta aaggccggca acccattgat caccattgac cagcgccctt tcgaggcatc
      481 attgcgtcaa gcccaggcca atcttgatcg tgaccgcgcc caactggggc aggtcgagtc
      541 cgccctggcc cagagcctgg cggcggagaa gcaggccgag gccaacctgg cgcgcgacat
      601 ggcccagctc cagaacgccc aggcccaaga gcgtcgctac aaggggttga tcgacgacgg
      661 ggccgtctcg agggagcagt acgatcaggt ccggaccgcc gctctggcgg cggaggcgac
      721 cgtccaggcg gatcaggccg cgatcaataa cgccaaggct gccatcgggg cggcgcaggc
      781 caccgtggaa aacgccaagg cagccatcca ggcggaccag gcggtggcgg agaacgcgcg
      841 gatccagctc ggctacacgg tgatccgctc gcccatcgac ggccgggcgg gtcagcggct
      901 ggtggaccct ggaaacgtgg tgaccgcgaa cagcaccgcg ctcctgacca tccagcgcct
      961 ggacccgatc tacgccgact tcaccgtcac cgaaaacgac ctcaccgcgg tccagcggaa
     1021 catggcccag gggaccctgc gggtcgaggt gcgcctcccg gatgagcctg acacacccag
     1081 aaccggccag ctcacgtttc tcgacaatgc cgtgcaagac gccaccggca cggtgaagct
     1141 ccgcgccacg atccccaacg acgatcatcg cttctggccg ggccgattcg tcaagatccg
     1201 cctcgtcctc cgcacgctgc aaggggcggt tctgattccc gcggacgcgc cccagatgtc
     1261 ggccaagggc ccctttgtgt acgtcgtcaa ggcggacgca accgccgagc tgcgccccgt
     1321 tgtgctcgga cagcgacagg gagaccggat cgtcatcgag cagggactca agcccggcga
     1381 gcgggtggtc atgacggggc agcttggagt gacacccggg ggcaaggtcc gcctccagga
     1441 acccggcgtc gggggaagtc cgcctgcggc cggcacggga gggaagtcgt gaacctctcc
     1501 gagcttttca tccgccggcc ggtcatgacg gcggtcctga ctctctccgt gatcctgttt
     1561 ggcgtgctga gctatctgcg cctcccggtg aacgatctgc cggccgtgga ctacccggtc
     1621 atccaggtgc aggcggatta cccgggcgcg agccccgaca cggtcgcgaa caacatcgcc
     1681 accccgctgg agcggcagtt catgcagatc aacggactgg aactggtgac ctccaagagc
     1741 acccagggtc acgcgagcct cacgctccag ttcgcgctgg acaagagcat cgacgccgcc
     1801 gccacggatg tccagacggc catctcacaa gccacaggca gcctcccggt ggacttgccc
     1861 tcgccgccca cgtactccaa gactaccccc aacgaccagc cgatccggta catcgccctc
     1921 cccagcgact cggtcacgcc agggcagctc tacgactatg ccagcac
//
LOCUS       DBNGZC010000922         2890 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1349837, whole genome shotgun sequence.
ACCESSION   DBNGZC010000922 DBNGZC010000000
VERSION     DBNGZC010000922.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1349837"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="AC16G0_15910"
     CDS             <1..832
                     /locus_tag="AC16G0_15910"
                     /EC_number="1.17.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HIR5101139.1"
                     /translation="TTDVSAALKRLDLVVVQHPFMTETAKHADVVLPVAGFGEEEVTF
                     TNTERRIQLVARAVDPPAGIIPAWQQISQMANAFGARWNYASAAEVMAEIGRAVPFYA
                     AASYDNLARDYGRQWPCTKDKPLGTRFLFEEGISGRPFAFVSIPRPDALPCASEDYPF
                     ALTFGHSLYYWHQNVLVQHSETLKREYQILLLDYPQGFVDINSEDAKELQIRDGQRVR
                     LVAATGSAVTSARVTHEVKRGMIFVPFFLGEVREQILGAAAHRPGSGPAALCVRVERA
                     "
     gene            834..1916
                     /locus_tag="AC16G0_15915"
     CDS             834..1916
                     /locus_tag="AC16G0_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5101140.1"
                     /translation="MRARVIGSTHILRLVDLLREEYEVIAPFCGKGRDSYFDTVSDAN
                     RDQVQIHLHNPYYPPKRYVLPHIEPLLTLHTNDRARIEPRTEAPKRALFGIRSCDTAG
                     IYHLDRFYLGRDFKDVYYERRRQNLFLVNVACTDPDQDIDDDCFCVCADTGPAARDHF
                     DLQLMDLGEDFLVVAGSPKGEALFANPLFRKATPAHVARRREILGEVRKRFKLATSWY
                     AATVRAITAGLVSESTWEQIGNRCLECGGCSYMCPTCTCFTVTERRTGPQEIERMRIW
                     DSCALGGFTRMAGGFNPRKPVHDRRNRRFIRKLAHYFIQRELSVSCVGCGRCVEVCHG
                     NIGMPTVVEIVRRAGAETERHEPVAH"
     gene            1897..2745
                     /locus_tag="AC16G0_15920"
     CDS             1897..2745
                     /locus_tag="AC16G0_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011736637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HIR5101141.1"
                     /translation="MSQLHTENVYLPKIAIVDRVVDEIAEVKTFRWHFEDPREQAAFK
                     KFRPGQFAQVSIFGAGEFPTSLPPSPTEDEVFFTIRRVGTVTTALHQLKPGDQFGVRG
                     PYGNGFPMEEYAGRNLVFVAGGIGLIPLRSCIIYALDNRERYGKIQLFYGAREPKDLM
                     YLDLLRQWEKTEGFECYLTVDRADQTWNGHVGVVGSLFREPGVSLPVENTTAFVCGPP
                     IMFRFVIKDLKVMGFQDKDIVSTLERYMKCGVGKCGHCCIGLAYVCTDGPVFTFEQIR
                     RLGEDI"
BASE COUNT          529 a          911 c          943 g          507 t
ORIGIN      
        1 caccaccgac gtctcggcgg cgctcaagcg gctggatctc gtggtggtcc agcacccgtt
       61 catgaccgag accgccaagc acgcggacgt ggtcttgccg gtagccgggt tcggagaaga
      121 ggaagtaacc ttcacgaaca ccgagcggag gatccagctc gtcgccaggg ccgtggaccc
      181 tcccgcaggg atcatccccg cctggcagca gatcagccag atggcgaatg ccttcggcgc
      241 tcgttggaac tacgcgtcgg ccgccgaggt catggcggag atcggacggg ccgtcccgtt
      301 ctacgccgcc gcgagctacg acaacctcgc cagggattac ggacgacagt ggccctgcac
      361 caaggacaag cccctgggca ctcggttcct tttcgaggaa ggcatctcgg gccggccctt
      421 cgccttcgtc tccattccac gacccgacgc cttgccgtgc gcgtccgagg attacccctt
      481 cgccttgacc ttcggccact cgctctatta ctggcatcag aacgtcctgg tccagcatag
      541 cgagacgctg aagcgagagt accagattct cctcctggat tatccgcaag gcttcgtgga
      601 tatcaacagc gaggacgcca aagagctcca gatccgcgat gggcaaaggg tccggctggt
      661 cgcggccacc ggctccgccg tcacctcggc gcgggtgacg cacgaggtca agcgcgggat
      721 gatcttcgtc cccttcttcc tcggagaagt gcgcgaacag atcttgggcg cggcggcgca
      781 caggcccggg agcggtcccg ctgccctgtg cgtacgcgtg gagagggcgt gagatgcggg
      841 cacgggtgat aggcagcacc catatcttga gactcgtgga cctgctccgg gaagagtatg
      901 aggtcatcgc ccccttctgc ggaaaggggc gggatagcta ttttgacacg gtgtcggacg
      961 cgaaccgcga ccaggtgcag atccacttgc ataatcccta ttatcccccc aagcggtacg
     1021 tcctcccgca catcgagccc ctgctgacgc tgcataccaa tgaccgcgcc cgaatcgagc
     1081 caagaaccga ggcgcccaag cgggccctct tcggcatccg atcatgtgac acggccggga
     1141 tctaccacct ggatcgattc taccttggcc gggacttcaa ggatgtctac tacgaacggc
     1201 gccgccagaa cctcttcctg gtgaatgtcg cgtgcacgga tccggaccag gacatcgacg
     1261 acgactgctt ctgcgtgtgc gcggacaccg gccccgccgc cagggatcac ttcgacctgc
     1321 agctcatgga cctgggcgag gacttcctgg tggtcgcggg ctcgcccaag ggtgaggccc
     1381 tgtttgccaa cccgctcttc cggaaggcga cacccgcgca cgtggcccgg cggcgcgaga
     1441 tcctcggcga ggtgcggaag cggttcaagc tggcgacaag ctggtacgcc gccacggtgc
     1501 gcgccatcac cgccggactg gtctccgaga gcacgtggga gcagatcggg aaccgctgtc
     1561 tggagtgcgg gggatgcagc tacatgtgtc ccacctgcac ctgcttcacg gtgaccgagc
     1621 gacgcacggg cccgcaggag atcgaacgca tgcgcatctg ggactcgtgc gccctggggg
     1681 gattcacgcg catggcgggt gggttcaatc cacggaagcc cgtccacgat cgccggaacc
     1741 gacgcttcat ccgaaagctg gcccactact tcatccagcg ggagctgtcg gtctcctgcg
     1801 tgggctgcgg gcggtgcgtg gaggtctgcc acggaaacat cggcatgcca accgtcgtcg
     1861 agattgttcg cagagccggt gcggagacgg agcgacatga gccagttgca cactgaaaac
     1921 gtgtatctcc caaaaatcgc catcgtggat cgggtggtcg acgagatcgc cgaggtcaag
     1981 acattccggt ggcacttcga ggacccgagg gagcaggcgg ccttcaagaa gtttcgaccc
     2041 ggccagttcg cccaggtgtc catcttcggg gccggcgagt ttcccacttc cttgccgccg
     2101 agtccgaccg aggacgaggt cttcttcacc atccggcggg tggggacggt gaccacggcc
     2161 cttcaccagt tgaagccggg cgaccagttt ggcgttcgcg ggccgtacgg gaacgggttc
     2221 cccatggagg agtacgccgg acgaaacctg gtgttcgtgg cggggggcat cggtctgatc
     2281 cccctgcggt cctgcatcat ctacgccctg gataaccggg agcgctacgg caagatccag
     2341 ctcttctatg gcgcccggga gccgaaagac ctcatgtacc tcgatctgtt gcgccagtgg
     2401 gaaaagaccg agggcttcga gtgctacctg acggtggacc gcgccgacca aacctggaat
     2461 ggccatgtcg gggtcgtggg aagcctgttc cgagagccgg gggtgagcct gccggtggag
     2521 aataccacgg ccttcgtgtg cggccccccc atcatgttcc ggttcgtcat caaggacctg
     2581 aaagtcatgg ggttccagga caaggacatc gtctccacgc tggagcgcta catgaagtgc
     2641 ggcgtgggaa agtgcggcca ctgctgcatc ggccttgcct acgtgtgcac ggacggcccg
     2701 gttttcacct tcgagcagat ccgaaggctg ggagaagata tctgatggag gatctccgca
     2761 gtgcactgag ggagaaattg cagggccgcg tctgccttgt tggcatcggc aatccggacc
     2821 tgggcgacga cggattcggg gtgcgcctgg ccgaggacat ccaggccgcg ggatatccgg
     2881 atgtggtcgt
//
LOCUS       DBNGZC010000923         2154 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1545327, whole genome shotgun sequence.
ACCESSION   DBNGZC010000923 DBNGZC010000000
VERSION     DBNGZC010000923.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1545327"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1240)
                     /locus_tag="AC16G0_15925"
     CDS             complement(<1..1240)
                     /locus_tag="AC16G0_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011034316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5101142.1"
                     /translation="MDYITDTLGQVLEAQVARHPEQEFVVYPDRNLRFTYAEFDRRVN
                     ALAKGLLAIGLTKGDHLGIWATNVPDWLTLLFATAKIGVVAVTVNTSYRAHELEYLVK
                     QSDIKALCLIDGFRDNDYLAIINDLVPELKSCERGRLQSARFPRLKTVIFIGPEKHRG
                     MYNTQELLLLGAHTSDEALAHAGASLSCHEVINMQYTSGTTGFPKGVMLTHHNILNNG
                     FHIGERQQFTEAERVCLTVPLFHCFGLVLALMAVITHGATLVVLESFDPLLALASVQK
                     EKCTAIYGVPTMFIAELNHPMFPMFDLSSLRTGIMAGSPCPMEIMKRVIADMKCRDIT
                     IAYGLTEGSPVMTQTSTDDPIELRVSTVGRRLPGIEVRIVNPETNAELPPGTQGEVVC
                     RGYNVMKGYYQLPEATEQAVD"
     gene            complement(1257..1832)
                     /locus_tag="AC16G0_15930"
     CDS             complement(1257..1832)
                     /locus_tag="AC16G0_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011868797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5101143.1"
                     /translation="MAEKNTIGGKLSLVRESRKLSQDQLAERAGLTAGMVKEIESGAL
                     IPSLGPLIRIARALGVRLGTFLDDQENVGPVLVRAGEKRKVMRVSDKNHPGHSDLDFF
                     SLAANKAGRHMEPFLIDIHPSSAQEVKMSAHEGEEFIFVFAGEVEITYGTETHRLSVG
                     DSIYYDSIVAHNVHCAGDQPAQILAVVYAPF"
BASE COUNT          376 a          678 c          700 g          400 t
ORIGIN      
        1 gatccacagc ctgctcggtg gcctctggca actggtagta gcccttcatc acgttataac
       61 ctcggcagac cacctccccc tgcgtccccg gcggcagctc cgcgttcgtc tcggggttca
      121 cgatccgcac ctcgatcccc ggcaggcgac gccccaccgt ggagacgcgc agctcgatgg
      181 ggtcgtcggt ggaggtctgg gtcatgaccg gcgagccctc ggtgaggccg taggcgatgg
      241 tgatgtcgcg gcacttcatg tccgcgatca cccgcttcat gatctccatg gggcacggcg
      301 agcccgccat gatgccggtg cgcagcgagg agaggtcgaa catggggaac atgggatggt
      361 tcagctcggc gatgaacatc gtgggcacgc cgtagatagc tgtgcacttc tccttctgca
      421 ccgacgccag cgccaggagc gggtcgaagc tctccaggac caccagggtg gccccatggg
      481 tgatgacggc catgagagcc agcaccagac cgaaacagtg gaacagcggc acggtcaggc
      541 acacccgctc ggcctcggtg aactgctgtc gctcgccgat gtggaagccg ttgttcagga
      601 tattgtggtg ggtgagcatc acgcccttgg ggaatcccgt ggtgcccgag gtatactgca
      661 tgttgatcac ctcgtggcat gagaggctcg ccccggcatg cgccagcgcc tcgtcgctgg
      721 tgtgcgcgcc caggagcaaa agctcctgag tgttgtacat gccccggtgc ttctccggcc
      781 cgatgaagat cacggtcttg agcctcggga aacgcgcgct ctggagccgc ccgcgctcgc
      841 aggacttcag ctcggggacc aggtcgttga tgatggccag atagtcgttg tcgcggaatc
      901 cgtcgatgag gcagagggcc ttgatgtccg actgcttcac caggtactcc agctcgtggg
      961 cacggtagga ggtattcacc gtgaccgcca ccaccccgat cttggccgtg gcgaagagca
     1021 gtgtgagcca gtcgggcacg ttggtggccc agatccccag gtggtcgcct ttggtgaggc
     1081 caatggccag gaggcctttc gccaaagcat tcacccggcg gtcgaactcc gcgtaggtga
     1141 aacgcaggtt ccggtcggga tagaccacga actcctgctc gggatgtcgg gcaacctggg
     1201 cctccaagac ctgaccgagg gtgtcggtga tgtagtccat gcggctcctc ccgggatcag
     1261 aacggcgcgt acaccaccgc caggatctgg gccggttgat ccccggcgca gtgcacgtta
     1321 tgagcaacga tgctgtcgta gtagatcgaa tccccgacgg acagtcggtg ggtttcggtg
     1381 ccgtaggtga tctccacctc gccggcgaac acgaagatga actcctctcc ctcgtgtgcc
     1441 gacatcttga cctcctgggc agaagagggg tggatgtcga tcaggaatgg ctccatgtgg
     1501 cggcccgcct tgttggccgc caaggagaag aagtccagat cactgtgccc gggatgattc
     1561 ttgtccgaga cgcgcatgac cttgcgcttc tcgcccgccc gcaccagcac gggtccgacg
     1621 ttctcctggt cgtcgaggaa ggtgccgagc cggaccccaa gcgctcgggc gatccggata
     1681 agaggtccga gggagggaat cagggccccc gactcgatct ccttgaccat tcctgctgtc
     1741 agtcctgccc gctccgccag ttgatcctgg ctcagcttcc gcgattcgcg caccagcgag
     1801 agcttcccgc caatcgtgtt cttctccgcc atatctcacc tcgccgccct gcgtggttgg
     1861 caggcccacc cggcccattc ggcccggacc cgcgtgaggc attctgcaca aatttccgat
     1921 ttgttcaagc aaatgtgacg ccgcccggga gaaaagtccg ggtcgtccgg ccggtcgtca
     1981 atgggaacta ttggtgaggg ggtattcggg tagaggctag ggcgtggccg ccaggctcac
     2041 gacggtatcc agcacgggct tcaggacctt gagcgcgagg aggccgaagg tatcgatcgc
     2101 caggtgcgca ccgatggccg gccagaggct gttccgccag accgccaggg cgcc
//
LOCUS       DBNGZC010000924         4752 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1254938, whole genome shotgun sequence.
ACCESSION   DBNGZC010000924 DBNGZC010000000
VERSION     DBNGZC010000924.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1254938"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="AC16G0_15935"
     CDS             <1..480
                     /locus_tag="AC16G0_15935"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR5101144.1"
                     /translation="FDIGILVGLAGYGWWVENPGLKIRGLAGGMTVAGAGLLDQVVKN
                     VACRARPNAPHAGDFFSNFPCFPASYSYASFPSGHATTAFATALILSFWCPRYTSLFL
                     TLAVLVGLSRVVLGAHFPSDVMAGAVLGVGVALVVSAYVPSLRMRFEKEVAVDGVSG"
     gene            513..1049
                     /locus_tag="AC16G0_15940"
     CDS             513..1049
                     /locus_tag="AC16G0_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101145.1"
                     /translation="MTRVGDIRIVFLILLGISLSACAGQGMGYPAAAPIPYPPPGFAH
                     RVETPQMGLFYNCTNPEPGLLRLEGLAFNVWNMQPIRYLELALEGVDRSERAVSEAGG
                     EGRDYQIPTGQSTPFQVDLRQMGTEVRYDLYYQYQFVDGDHRDLISGPMVRGVHPRHS
                     GIRFLARDACSPTQHVFR"
     gene            1181..1402
                     /locus_tag="AC16G0_15945"
     CDS             1181..1402
                     /locus_tag="AC16G0_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006195783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HIR5101146.1"
                     /translation="MPRLDDYTTVTRPDDNGTYVAYVPAIPGCHAIGKTPDEARSELA
                     NVFAMIAEEYAEEGRALPPDVGALVARAS"
     gene            1392..1607
                     /locus_tag="AC16G0_15950"
     CDS             1392..1607
                     /locus_tag="AC16G0_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006195784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HIR5101147.1"
                     /translation="MPAKSGDLERVAKTLGFVKSRQRGSHARWKHPDGRATTIPIHPS
                     LEVGGWLFHEILKQLGISEQDFEQLKR"
     gene            1830..3458
                     /locus_tag="AC16G0_15955"
     CDS             1830..3458
                     /locus_tag="AC16G0_15955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR5101148.1"
                     /translation="MLETGDWVTPHFNGQPRFDKPILFYWLLTLSYRVFGVTEFAARF
                     WSALAAVTLVGMLSWAGQRWFGPLAGLLTGMAFTANLLSALLARAAVTDMLLTLFVTT
                     AILAGVWAIQADVVPGRRWTRTAWAAMGLAVLVKGPVGLLIPAMALGGSLLIFREWRT
                     GIRRLLSWDSVILFGLITAPWYGLVLLANGRAFVDGFVVKHHLTRFAGVVSGHPGPLW
                     FYLPVALLGFFPWSAYLPQALWMAVRIGRTREVKDPADRLLVACACWALGVFLFFSLA
                     GTTLPSYLFPAFPAMALLVGASAISNEKRTTDNRPRRDVDRLSVVGSQFSIASVRRER
                     VPEWPSRLSSWLIGITGFALAGGFALVPVFLDRFRPLAGGVLDGVALPAGIAWWLAGL
                     LAVGTTASLVGAGAWRLAFLSVMMVLLLFTAEIVVAPRAYAILQAALHDFAQEAGRVL
                     GPRDPVVAYGLNAPSIVFYSGRRVIPLGASAEGEQQLQRMAETGRLVVIITRISQVGR
                     LDRLPGLIRLGSRGGYAAYSTVRGGGTPGGRPRG"
     gene            3492..4490
                     /locus_tag="AC16G0_15960"
     CDS             3492..4490
                     /locus_tag="AC16G0_15960"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR5101149.1"
                     /translation="MSPLISTSAQNGLFTVIADELQAVEDRLQTPGEESGTLLRVVAN
                     YALASGGKRIRPALLLLAARACGATQTERLVTLASVAEVMHAASLIHDDIVDHSEMRR
                     GRPSAAARWGPDVSVLVGDFLYSRAIQILVNDGDMRILQAFADATLAMTEGEVLQLQY
                     LHDLKISEANYLRIVTGKTAALMAAACRAGALVADAPERRVDAFGAFGLNLGIAVQLV
                     DDALDYSACETRLGKPVGSDFREGKITYPIIHVMRAASLEDRAILESLPGRVGGGNGD
                     LSPLHALVDRYEGVQATLHLADKYLAQAHTQLHGVPESPACRALHRLADFVRERDR"
     gene            4515..>4752
                     /locus_tag="AC16G0_15965"
     CDS             4515..>4752
                     /locus_tag="AC16G0_15965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR5101150.1"
                     /translation="MENGQSTTDQRSLRGDELADRLLDFSVQVITLASRLPKNAPGRH
                     ISVQMVEAETSVGAHYEEARGAESKADFVHKLGIA"
BASE COUNT          768 a         1445 c         1558 g          981 t
ORIGIN      
        1 tttgacattg ggattctggt agggctggct gggtacgggt ggtgggttga aaatcctgga
       61 ctgaagatac gagggctggc gggggggatg acggtggcag gcgcgggact cctcgatcag
      121 gtcgtgaaga acgtcgcgtg ccgcgccagg ccaaacgccc ctcacgcggg agatttcttt
      181 agcaacttcc cgtgcttccc ggcgtcttat agttacgcct catttccgtc gggacatgcg
      241 accacggcct ttgcgacggc tctcatcctc agcttctggt gcccgagata cacgagcctt
      301 ttcctgacat tggccgtctt ggtcgggctg tcgcgcgtgg tgctgggggc gcatttccca
      361 tccgatgtca tggcgggagc ggtgcttggc gttggagtag ccctggtcgt ctccgcctat
      421 gttccgtcgc tacgaatgcg gtttgaaaag gaagtggcag tggacggagt ctccgggtga
      481 ttaggtcggc ggccgcgggg gaggattgtt caatgacaag agtgggcgac attcgaatcg
      541 tgttcttgat cctcttgggg atcagcctgt cggcgtgtgc cgggcaaggg atgggatacc
      601 cggcggccgc tcctatccct tatccaccgc cagggtttgc ccacagggtg gaaaccccac
      661 agatggggct tttctataat tgcacgaacc ctgagcctgg tctgcttcgg ctggagggat
      721 tggccttcaa cgtgtggaat atgcaaccca tacgatacct cgagctggca ttggagggcg
      781 tggatagaag cgagcgcgcg gtgtccgagg ctggcggaga aggccgggac taccagattc
      841 ccacagggca aagcacgcca ttccaggtgg atctgcggca gatgggtacc gaggtccgtt
      901 acgatctcta ctaccaatac caattcgtgg atggagacca ccgggatttg atctctggac
      961 ccatggtgag gggagtgcac ccccgacact ccggaattcg attcctggca cgcgacgcct
     1021 gttcgcccac gcagcacgtc tttcgctgag gtcaaagacc tagcccagta tctgtgcgcg
     1081 tcgttcccgg ttgaaggggg gggcatcggt gtggtacaag gagagacagg tgtagactgc
     1141 caggaagcgc gttctggtgc ctgcacttgg gagaaagcgg atgcctcgat tggatgacta
     1201 cactacggta acccgaccgg atgacaatgg cacctacgta gcatacgttc ctgcgattcc
     1261 agggtgtcac gctatcggta agactccgga tgaggccaga tccgaattgg cgaatgtctt
     1321 tgccatgatc gcggaagaat acgcggaaga ggggcgggcg ctgccacccg atgttggagc
     1381 gctggtcgcc cgtgccagct aagtctggcg acttggagcg tgtcgcaaag accctcgggt
     1441 ttgtcaagtc ccgccaaagg ggtagtcatg cgcgctggaa gcatccggac ggtcgagcca
     1501 caacgattcc gatccatcca tccctggagg ttggagggtg gctgtttcac gagattctca
     1561 agcaacttgg aatttccgag caggacttcg agcagctcaa gcgctagctc gtcgcccccg
     1621 tctgattcct ccctcatgac cacgtcctcg cctctgagcc agtgacctca tctccgctcc
     1681 aagaactatc aaaccacgcg cgttgctgga gcactgtcac gactctcctt gctcttgttc
     1741 tcctctcggg ctcactcttc ttcttccgcc tcgggactcc cggcctcttt gacgcggacg
     1801 agccggcgta cgcccaggcc gcccgcgaga tgttggagac cggggactgg gtcacgcccc
     1861 attttaatgg acaaccccgc ttcgataagc ccattctgtt ctactggctc ctcacgctga
     1921 gctatcgtgt gttcggcgtg acggagttcg ctgcgcgatt ctggtcagcc ctggccgccg
     1981 tgaccctggt cgggatgctc tcctgggcgg ggcaacgatg gttcggaccg ttggcgggtc
     2041 ttctcaccgg catggcgttc acggcaaatc tgctcagcgc cctcttggcg cgtgccgcgg
     2101 tgaccgacat gctgctcacg ctttttgtca ccacggccat cctggcgggc gtctgggcga
     2161 tccaggcgga cgtggttccg ggacggcgct ggactcgcac ggcttgggcg gccatgggtc
     2221 tggcggttct ggtgaaaggg cccgtgggcc tgctgatccc ggccatggcc ctcggaggat
     2281 ctctgctcat cttccgggag tggcggacgg ggattcgccg cttgctctcg tgggactccg
     2341 tgatcctctt tggattgatc accgccccat ggtacggcct ggtccttctg gcgaacggtc
     2401 gggccttcgt cgacgggttc gtggtcaagc atcacctgac gcgtttcgcc ggggtggtct
     2461 ccggccaccc cggccccctc tggttctatc tccccgtggc gctgctgggg ttcttcccct
     2521 ggagcgccta tctcccccag gccctgtgga tggcggtgcg catcggccgt acccgggagg
     2581 tgaaagaccc ggccgaccgt ttgctggtcg cgtgcgcctg ctgggccctg ggcgtgtttc
     2641 tgttcttctc cctggcggga accacgctgc cgagctacct ctttcccgcc ttcccggcca
     2701 tggccctcct cgtcggcgcg tcggcaattt caaatgagaa acggacaacc gacaaccggc
     2761 caaggagaga cgttgatcgg ttgtccgttg tcggttctca gttttcaatt gcctctgtcc
     2821 gcagagagcg tgtgcccgaa tggccctcgc ggttgtcgtc ctggctcatc gggatcactg
     2881 gcttcgcgct ggcgggcggc ttcgcgctgg taccggtgtt cttggatcgc ttccggccgc
     2941 tggcaggggg cgtcctcgac ggtgtcgccc tccccgccgg gatcgcgtgg tggcttgcgg
     3001 gcctcctggc ggtggggacg actgccagcc tcgtgggggc gggagcctgg cgcctcgcct
     3061 tcctctcagt catgatggtc ctcctcctct tcacggcgga gatcgtggtc gcacccagag
     3121 cctacgccat cctccaggct gcgctccatg atttcgccca ggaagcggga cgggtcctgg
     3181 gtccgaggga tccggtggtc gcctatggac tcaacgcacc aagcatagta ttctactccg
     3241 gccgtcgggt gataccgctg ggcgcctccg cggaagggga acagcagttg caacggatgg
     3301 ccgagacggg tcgtttggtc gtgatcatca cgcggatcag tcaggtcgga cgtctcgatc
     3361 ggcttcccgg cctgattcgt ctggggtcgc gcgggggcta tgcggcttat tccactgtgc
     3421 ggggaggtgg tacgcccggc ggacggccgc gcgggtgatc ggcacgacca accggttccg
     3481 atcaagctac ggtgagtcca ttgatctcca cgtctgcaca gaacgggtta ttcaccgtca
     3541 tcgccgatga actccaggca gtcgaggatc gtctgcagac ccccggagaa gagagcggca
     3601 cgctcctccg cgtggtcgcg aattacgccc tggcctctgg gggaaagcgg atccgtcccg
     3661 ccctcctgct cctggcggcg cgggcgtgtg gtgccacaca gacggagcgt ctcgtgacgc
     3721 tggcctcggt ggcagaggtc atgcacgcgg ccagtctgat ccatgacgat atcgtggatc
     3781 actccgagat gcgccggggt cgcccctctg cggcggcccg ctgggggccg gatgtctccg
     3841 tgctggtggg agacttcctc tactcccggg cgatccagat cctggtgaac gacggggaca
     3901 tgcgcatcct gcaggccttc gcggatgcga cgctggccat gaccgagggg gaggtgctgc
     3961 aacttcagta tttgcacgac ctcaagatca gcgaggccaa ctacctccgg atcgtcacgg
     4021 ggaagacggc ggccctgatg gccgccgcct gccgcgccgg cgccttggtg gcggatgctc
     4081 ccgaacgcag ggtggacgcc ttcggggcct tcggcctcaa tctggggatc gcggttcagt
     4141 tggtggacga cgccttggac tacagcgctt gcgagacgcg cttgggcaag ccggtgggga
     4201 gcgatttcag ggagggaaaa atcacgtacc cgatcatcca tgtgatgcgc gctgcctccc
     4261 tggaggatcg ggcgatactg gagagcctcc ccggccgggt gggtggcggc aacggcgacc
     4321 tgagcccgct ccacgccctc gtggaccggt acgagggggt gcaggccacc ttgcatcttg
     4381 cagacaagta cctcgctcaa gcccacaccc aattgcatgg ggtgcctgaa tcgccggcct
     4441 gccgcgccct gcacagactc gccgatttcg tccgagagcg cgaccgctga gccgcaaagg
     4501 ccgcggaatt ccaaatggaa aacggacaat cgacaaccga tcaacggagt ctgagaggag
     4561 atgagcttgc tgatcggctg cttgatttca gcgttcaagt gatcacgctg gcttctcgat
     4621 tgccgaaaaa cgctcccggt cgccatatct cggttcagat ggtggaggcc gagacttccg
     4681 tgggtgccca ctacgaagag gctcgggggg cggagagcaa ggcggacttc gtccacaagc
     4741 tcggcatcgc cc
//
LOCUS       DBNGZC010000925         2864 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2033757, whole genome shotgun sequence.
ACCESSION   DBNGZC010000925 DBNGZC010000000
VERSION     DBNGZC010000925.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2033757"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="AC16G0_15970"
     CDS             <1..812
                     /locus_tag="AC16G0_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIR5101151.1"
                     /translation="THTVYPVPHELKSHLLLELLRQGAARSILAFTRTKHRANRLADF
                     LERHGIACARIHGNRSQAQRTEALAGFKRGQFRVLVATDIAARGIDVEALGLVVNFDV
                     PHIPEDYIHRVGRTARVEATGDAYTFVSPDEEGGLRAIERHIGKRLPRPKVAGFDYEK
                     RPAERLEIPLAERIAAIRARKTDERARARANAERRTGQGVPARSAGNGRPKWGSPGAS
                     RAGSAGSKRVGAGLAWLRQSTGQADRGPVAGSLRRPGANRSAFSGRSGPTR"
     gene            1081..2016
                     /locus_tag="AC16G0_15975"
     CDS             1081..2016
                     /locus_tag="AC16G0_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-alpha-hydroxyglycine alpha-amidating
                     lyase family protein"
                     /protein_id="HIR5101152.1"
                     /translation="MLYGKGKYRYELVDPWAKLPEGDSFFDVGGVCVDGQDRVYVLSR
                     STQPITLFDRDGQIVLRWGQGFFGRAHGSCVGGDGSFYCTDDRKHVVTKFTPDGGTIL
                     MTLGTKDKPSLTGYRETPHLFERIASITHGGPPFNRPTGVAISPTGDIYVSDGYANAR
                     IHRFNSKGELISSWGEPGPGPSQFRLPHNLWVDASSRVWVADRENSRIQIFREDGKFL
                     TQWTDLIRPTDVCIHDDAVFVTELCRRVSIFNLDGELLSRWGNEGHPVESPLFYAPHA
                     VAVDSRGDLYVGEVSMTEAKVDRGPRALQKFARRA"
     gene            complement(2242..2778)
                     /locus_tag="AC16G0_15980"
     CDS             complement(2242..2778)
                     /locus_tag="AC16G0_15980"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR5101153.1"
                     /translation="MTATSGSLGKVKQIYRGRVVNLNVETVTLPNGATVELEVIRHQG
                     AAAVVPLKDDGSVILIRQYRHATGGYIYEIPAGKLDPGEDPRHCAARELEEEIGCRAS
                     SLTRLLSIFTTPGFTDEVIHIFLAAGLSPGTQRLDHDEVLEVVEMPLDLAIARIADGT
                     ILDGKTIVGLQAVYLNRK"
BASE COUNT          475 a          920 c         1004 g          465 t
ORIGIN      
        1 tcacccacac ggtctaccct gtcccccacg agttgaaatc gcacctcctg ctggaactcc
       61 tgcggcaggg cgcggctcgg agtatcctgg ccttcacgcg gaccaagcac agggccaacc
      121 ggctggccga tttcctggaa aggcacggca tcgcctgcgc ccgcatccac ggcaaccgca
      181 gccaggcgca gcggaccgag gcgctcgccg gcttcaagag gggacagttc cgggtcctgg
      241 tcgccacgga cattgcggcc cgcggcatcg atgtggaggc gctggggctg gtcgtgaact
      301 tcgatgtccc tcatattcca gaagattaca ttcatcgagt cggccgcaca gcgcgagtcg
      361 aggcgacggg cgatgcctac acgttcgtct ccccggacga ggaggggggc ctgcgcgcca
      421 tcgagcggca catcgggaag cgcctgccgc gcccgaaggt cgccgggttt gactacgaga
      481 agcggcccgc ggagcggctg gagattcctc tcgccgagcg catcgccgcc atccgtgcgc
      541 ggaagactga cgagcgggcc cgggcgagag ccaatgccga gcgccgcacc ggccaagggg
      601 tcccggcgcg gtcggccggg aacggaaggc cgaagtgggg ttctccggga gcgtcgcgag
      661 cgggctcggc gggttcaaag cgtgtcgggg cggggctggc ctggctgcga cagagcacgg
      721 gacaggccga ccgtgggcct gtcgcgggta gcctccggcg gccaggagca aaccgctccg
      781 ccttcagcgg gcgatccggc cctacgcgat gaggcagcgg atgcgcgcct ccgcgtgagt
      841 caaccagacc gccggtcgga cttccgaccg gcgtcgagcg ccatcagccg gcccggcggc
      901 aaagggtcgt tggacatggg cccacagcgt ctttcgcctt gacttcactc cccgaccgga
      961 tgtagcgtcc cgccgtccta tggcgtcccc gacagcggca gggagccgcc caaatccggc
     1021 aaggccagtc gcagtgattg gcactctgtg tatccatcct tcagggagga gtgcgcaggc
     1081 atgctttacg gcaagggaaa gtatcggtac gagctcgtgg atccgtgggc gaagctgccc
     1141 gagggggatt cgttcttcga tgtcggcggg gtgtgcgtgg acggccagga tcgcgtgtac
     1201 gtcctgagtc gcagcacgca gcccatcacc ctctttgatc gcgacggcca gatcgtcctg
     1261 cggtgggggc agggattctt cggccgggcg cacggcagtt gcgtcggggg cgacgggtca
     1321 ttctattgca ccgacgaccg gaagcacgtg gtgacgaagt tcacaccgga tggcggtacc
     1381 atcctcatga ccctgggcac caaggataaa ccctccctta cgggttaccg ggaaacgccg
     1441 cacctcttcg agcggatcgc atccatcacg cacgggggac ccccgttcaa ccggccgacg
     1501 ggcgtagcga tctcgccgac gggcgacatc tacgtctccg atgggtatgc gaacgcgcgg
     1561 atccatcgct tcaactccaa gggggagctg atctcgtcgt ggggggagcc gggccccggg
     1621 ccgtcccagt tccggctgcc ccacaacctc tgggttgacg cctcgagtcg cgtgtgggtg
     1681 gcggaccggg agaacagccg gatccagatc ttccgggagg acgggaagtt cctcacccag
     1741 tggaccgacc tcatccggcc gacggacgtg tgcatccacg acgatgccgt gttcgtgacg
     1801 gagctgtgtc ggcgcgtcag catcttcaac ctggatgggg agctgctgtc gcgctgggga
     1861 aacgaggggc atccggtgga gagccccctc ttctacgccc cgcacgccgt cgcggtcgat
     1921 tcccggggag atctgtacgt cggggaagtg agcatgaccg aggccaaggt ggatcggggc
     1981 ccgcgcgccc tgcagaaatt cgcccgccgg gcgtgacgat tgggcgtctg ccgcccgccc
     2041 tgacatcgca gccacactcc cacggccctc cggagccata cgcgctactg ggggccgttc
     2101 gttttcatcg tccaagcgga tggcagttgc ggcaatagag ggaaatggct ctgtgtgcga
     2161 aagtgagcaa ggaagtctgg ctttgctagc ctggggggcg cgtctctgca gtcgacagaa
     2221 atcagccgag ggggccgtcc atcatttccg gttgaggtac acggcctgga gaccgacgat
     2281 ggtctttccg tcgaggatgg tgccgtcggc gatccgggca atggccaggt ccagcggcat
     2341 ctccacgacc tccagcacct cgtcgtgatc caggcgctgc gtgccggggg agaggccggc
     2401 ggccaggaag atgtggatca cctcgtcggt gaaccccggc gtggtgaaga tgctgagaag
     2461 gcgcgtgagg gacgaggcgc gacagccgat ttcctcctcc agctcgcggg ccgcgcagtg
     2521 gcgcgggtcc tccccgggat cgagcttgcc ggcggggatc tcgtagatgt atccgccggt
     2581 ggcgtgccgg tattgccgga tgaggatcac gctaccgtcg tccttcagcg ggaccacggc
     2641 ggcagcgcct tggtgccgga tgacctccag ctccaccgtg gcgccattgg ggagggtcac
     2701 ggtctccacg ttcaggttga ccacccgacc ccggtaaatc tgcttcacct tcccgaggga
     2761 tcccgacgtg gcggtcatgg cgccacctgg atccgaccgt cggcaacggg cgcgatggga
     2821 ccggccttga ggcgatccag gatgaggtcg gaatccatgg gccc
//
LOCUS       DBNGZC010000926         3894 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2134152, whole genome shotgun sequence.
ACCESSION   DBNGZC010000926 DBNGZC010000000
VERSION     DBNGZC010000926.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2134152"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="AC16G0_15985"
     CDS             <1..610
                     /locus_tag="AC16G0_15985"
                     /inference="COORDINATES: protein motif:HMM:NF013811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR5101154.1"
                     /translation="LAALLNGRSLFSRVQAAGGEATFANAYTPRFLEKPPRFLSVSTV
                     ATGQAGLRFRTLEDLARGEAVFHDFTNRLLPERGYYLPPVTPGEAGGRLAKLAEAHTF
                     TMYEHFLPDLAGHAQDMERGVKLLEDLEAFLDAVLSGLNLSRTLVVLTSDHGNLEDLS
                     TKRHTRNPVPTLLWGRGAAEAVEGIRSLPDISLAILQHLNML"
     gene            657..2009
                     /locus_tag="AC16G0_15990"
     CDS             657..2009
                     /locus_tag="AC16G0_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR5101155.1"
                     /translation="MRPATTSGVPIRITILLVILALCPFIPTQASARQVNVIRVESVI
                     SPSSADYIVSAIKEAEKDKVAALVIEMDTPGGLDTSMRAIIKEMLAAERPIIVYVAPS
                     GARAASAGAFITMAANVAAMAPGTNIGAASPVALGGEMGKTMEKKVTNDAAAYMRTIA
                     EKRGRPIDLAEEWVRKATSKTETEALKAHLIDVVSPKLEDLLQTIDGRVVTTAAGKVK
                     IETKNAIIHREEMNLREKVLRIITDPTIAYLLLILGLAGLYFEFSTPGAVLPGILGGI
                     SLILALYAFQQLPINYAGVLLILLAIVMFIAEIKVVSHGVLTLGGIAAMILGSMMLID
                     TTSAPYLRISIGAILATAGATAAFFLFVVGAGVKALHSRTATGQEGLVGQVGVARTRL
                     EPRGQIYLRGELWNAECEQAIEPGESVRVAAVDGLTLRVVPIQAATAAPDAVATGRSG
                     "
     gene            2006..2800
                     /locus_tag="AC16G0_15995"
     CDS             2006..2800
                     /locus_tag="AC16G0_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HIR5101156.1"
                     /translation="MNLLPVALVPTIFIIIVLLYILSSAVKVLREYERAVIFRLGRLS
                     KAIINPGGNGNGPGLILLIPIIDKMVKVPLRTVTMDVPSQDTITKDNVSLKVNAVIYF
                     RVMDAERAVVAVEDYLFATSQMAQTTLRSVLGQSELDELLSERDKINQKLQRIIDEHT
                     EPWGIKVSAVEVKFIDLPQEMQRAMAKQAEAEREKRAKIIHAEGELLASEKLAQAAQI
                     IASEPVTIQLRYLQTLTEIGTEKNSTIIFPLPIDMLRALMGDRRPA"
     gene            2923..3801
                     /gene="folP"
                     /locus_tag="AC16G0_16000"
     CDS             2923..3801
                     /gene="folP"
                     /locus_tag="AC16G0_16000"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014737613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR5101157.1"
                     /translation="MTAYANLGGVEVGDEFPVRLIGVINVSPESFYQGSVAQGEDSLR
                     QKADQMLAEGADLLDIGAKSTAPYLPTEIGEAEEVRRLTWAVGLVRKVTTLPISADTT
                     RSRTAQAALDAGAQVINDVSGFRGDPEMAELVARRAQGVILMASGADPEARDPMGTVR
                     GLLEESLKITWKAGVPEHRVLLDPGIGFFRQAAIPWDTWDCEILRRLGELRVLGRPLL
                     VGVSRKSFIGKILGKSDPAERLVGSLAATAVAVVNGAHLIRTHDVGATREAVRMAEAL
                     RPRYDGGGRRAQGSSL"
BASE COUNT          725 a         1272 c         1237 g          660 t
ORIGIN      
        1 gctggccgcg ctgttgaacg gccgatctct cttcagccgc gtgcaggccg cgggtggcga
       61 ggcaaccttc gccaatgcct ataccccgag attcctggag aaacccccgc gcttcctctc
      121 ggtaagcacc gtggccacgg gacaggcagg actgcgattc cggacgctgg aggaccttgc
      181 caggggggag gccgtctttc acgacttcac caaccggctg ctccccgagc gcgggtatta
      241 cttgccgccc gtgacgccag gggaggcggg gggccgcctc gccaagctag ctgaggccca
      301 caccttcacc atgtacgagc acttcctgcc cgacctggcc ggccacgccc aggacatgga
      361 acgaggagtc aagctcctcg aagacctgga ggcgtttctg gacgcggtcc tatccggcct
      421 gaatctctcg cgaacattgg tggttctgac cagcgaccac ggcaacctcg aagatctttc
      481 cacgaagcgg cacacgcgga atccagtccc caccctgctc tgggggcgag gcgctgccga
      541 ggcagtcgag ggcatccgca gcctgcccga tatatccctg gctatcttgc agcacctgaa
      601 tatgctatga tacgcgcata aaaggataag gggccatgcc ggccccccga cttcccatgc
      661 gtcctgcgac cacgtcgggc gttcccatcc ggatcacgat cctccttgtg atccttgccc
      721 tgtgtccgtt catcccgacc caagcctccg cgcgtcaggt caacgtcatc cgagtggaga
      781 gcgtgatctc ccccagctct gccgactaca tcgtcagcgc catcaaggag gccgagaagg
      841 acaaggtggc cgccctggtc atcgagatgg atacccccgg cggcctggac acgtccatgc
      901 gggccatcat caaggagatg ctggcggccg agcgaccgat catcgtctac gtggcgccca
      961 gtggggcgcg cgctgcctcc gccggcgcct tcatcaccat ggccgcgaat gtggccgcca
     1021 tggctcccgg aacgaacatc ggcgccgcgt cccccgtggc cctaggcggt gagatgggca
     1081 agaccatgga gaaaaaggtc accaacgacg cggcggccta catgcggacg atcgcggaga
     1141 agcgagggcg ccctatcgac ttggccgagg aatgggtgcg caaggccacc tccaagacgg
     1201 aaaccgaggc cctgaaggcc catctcatcg acgtggtaag ccccaagctg gaggatctgc
     1261 tgcagacgat cgacggccgg gtcgtgacca cggcggcggg gaaggtcaag atcgaaacca
     1321 agaatgcgat cattcaccgg gaggaaatga acctccgcga aaaggtgttg cggatcatca
     1381 ccgacccgac catcgcctac ctgcttctga tcctgggcct ggccggcttg tatttcgagt
     1441 tcagcacgcc cggcgccgtc ctgcccggca tcctgggcgg gatcagcctg atcctggccc
     1501 tctacgcctt ccagcagctt cccatcaatt acgccggggt cctgctcatc ctcctggcca
     1561 tcgtgatgtt catcgcggag atcaaggtgg tctcccatgg ggtgctcacc ctgggcggaa
     1621 tcgcggcgat gatcctgggc tccatgatgc tcatcgacac cacctctgcg ccctacttgc
     1681 ggatctccat cggcgcaatc ctggcaacgg ccggcgccac ggctgccttc ttcctcttcg
     1741 tggtgggcgc cggggtcaag gccctgcact ccaggaccgc cacgggccag gaggggctgg
     1801 tgggccaagt cggtgtggcg cgcaccagac tcgagccgcg ggggcagatc tatttgcgtg
     1861 gagaactgtg gaacgctgag tgtgagcagg caatcgagcc cggcgagtcc gtccgagtcg
     1921 ccgcggtgga cggcctcacg ctccgggtgg ttccgatcca agccgcgacg gctgcacccg
     1981 acgccgtcgc cacggggagg tcaggatgaa cctgcttcca gttgccctcg tacccacgat
     2041 cttcatcatc atcgttctcc tctacatcct ctccagtgcg gtgaaggtgc ttcgggaata
     2101 cgagcgggcc gtcatctttc gactgggcag actgtccaag gcaatcatca acccgggggg
     2161 caacggcaac ggtcccggac ttatcctgct gatccccatc atcgacaaga tggtgaaggt
     2221 gcctctgcgg acggtaacca tggacgtgcc ctctcaggac accatcacca aggacaacgt
     2281 ctctctcaag gtgaatgccg tcatctactt ccgggttatg gacgcggaac gggcggtggt
     2341 ggccgtggag gactacctct ttgccacctc tcagatggcc cagactacct tacgcagcgt
     2401 cctgggccaa tccgagctgg acgagctcct ctcggagcgg gacaagatca accagaagct
     2461 gcagcggatc atcgacgagc acaccgagcc ctgggggatc aaggtcagcg ccgtggaagt
     2521 gaagttcatc gatcttcccc aggagatgca gcgcgccatg gccaagcagg ccgaggccga
     2581 gcgggagaag cgcgccaaga tcattcacgc cgaaggggaa ttgctggcct cggaaaagct
     2641 cgcccaagcc gcccagatca tcgcttccga gcccgtgacc atccagctcc gctacctcca
     2701 gaccctgacc gaaatcggga cggagaagaa ctcgactatc attttccccc tgcctatcga
     2761 catgctccgc gccctcatgg gggacagacg gcccgcctga aggccgcccc ggacattggt
     2821 ctcctcgacg gcgtcaggga tcgccccctg gcgccgtttg gatttctggg tgggacaggg
     2881 ggcaaaggcc tgggggcgac cccctgacac gggaggggat acatgaccgc ctatgccaac
     2941 ctggggggag tggaggtggg tgatgagttt cccgtccgcc tcatcggtgt gatcaatgtg
     3001 agtcccgagt cgttctacca gggctcggtc gcgcaggggg aggattcgct tcgacagaag
     3061 gcagaccaga tgctggccga gggggcagat ctcctcgaca ttggggccaa gtccacggcc
     3121 ccctatctgc caaccgagat cggcgaggcc gaggaggtcc ggcgcctcac ctgggcggtc
     3181 ggcctcgtgc ggaaggtcac gaccctcccc atttcggccg atacgacccg gagccggact
     3241 gcccaggctg ccttggacgc gggggcccaa gtcatcaatg atgtgagcgg cttccgcggc
     3301 gaccccgaaa tggcagaact tgtcgcacgc cgagcccagg gagtgatcct gatggctagc
     3361 ggcgccgacc ccgaagcgcg agaccccatg gggaccgtgc gaggactgct ggaagagagc
     3421 ctgaagatca cctggaaggc cggcgtcccg gagcaccggg ttcttcttga tcccggcatc
     3481 ggattcttcc gccaggcagc aatcccctgg gatacgtggg actgcgagat ccttcgccgc
     3541 ttgggcgaac tgcgcgtgct cggccggccc ctgctagtgg gtgtgtccag gaagtccttc
     3601 atcgggaaga tcctcggaaa gtcagatccc gccgaaaggc tcgtgggatc gttggccgca
     3661 accgccgtgg cggttgtgaa cggtgcccac ctgatccgca cacatgacgt gggcgcgacg
     3721 cgcgaggcgg ttcggatggc ggaggccctg cgcccgcgct acgacggcgg tgggcggcga
     3781 gcccagggca gcagcctgtg actggggacc ggaccaatgc aggatgaatt gttgctggca
     3841 gccctggaaa cattggcaga ccagctggga atcccggtga cctacgccac gctg
//
LOCUS       DBNGZC010000927         4361 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1642861, whole genome shotgun sequence.
ACCESSION   DBNGZC010000927 DBNGZC010000000
VERSION     DBNGZC010000927.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1642861"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /locus_tag="AC16G0_16005"
     CDS             <1..1494
                     /locus_tag="AC16G0_16005"
                     /inference="COORDINATES: protein motif:HMM:NF013727.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR5101158.1"
                     /translation="PKPPKEGKVQQAFSFLAEKGGPINPSLKLLDLPSAEPVGSSPEE
                     AAENSRILEKKLQEFGVEGRVTAVNPGPVITSYELEPGPGIKINRIVALADDLALGLK
                     ALSVRVVAPIPGKAAVGVEIPNQNRATVHLREVLAAKEFANESLHLPLALGKEIGGAP
                     VVADLTQMPHLLIAGETGSGKSVCINALILSLLYRSQPKNVRLLLIDPKRVELSVYNG
                     IPHLADKVVSDTKDAARRLQRVVVHMEERYKLFAQVGARNLQSYNRRMELEPPTPDDL
                     AQGSYRGQLPMLVVVIDELADLIMAAQSDVENAIMRLAQMARAVGIHLIVATQRPSVD
                     VLTGVIKANFPARISFRVASKVDSRTILDMNGAEALLGKGDMLYIPPGSSKPIRIHGC
                     NVTEVEIRRVVDFLAAQSKAPEFVWSLLPPEEEAPVEDEEGDELYRQAVEMVVQTQQA
                     SISMIQRRLRVGFNRAARMIERMEREGIVSPMDGTRPREVLVSRAES"
     gene            1496..2212
                     /gene="lolA"
                     /locus_tag="AC16G0_16010"
     CDS             1496..2212
                     /gene="lolA"
                     /locus_tag="AC16G0_16010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein periplasmic chaperone
                     LolA"
                     /protein_id="HIR5101159.1"
                     /translation="MRYGSFIPAVALGVLLEVGIGWPANGPAWLPGAPVAEAAPSLNE
                     VVDKVRETCSHAQDLSALFEQITTIRSLNQEQRASGILLLKRPRKMRWEYQKPESRLF
                     VTDGKTLWAYSPADKQVVVQEVGEAFASRLPISILAGDCQLRRDFDIREVENAATRAS
                     GNVMILDLRPKQSEAGITRMLLEVNLKTYTIERITVFDASGNTSVYKLSDLRLNPGLS
                     DQQFTFTPPAGVTVVNPPQQ"
     gene            2259..2753
                     /locus_tag="AC16G0_16015"
     CDS             2259..2753
                     /locus_tag="AC16G0_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005376039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HIR5101160.1"
                     /translation="MAKECSFDIASKVDMQEVDNAVHQTTREISQRFDFKGSQAEITR
                     EGNSLKLGAEDEFKLKQILDILETKLVKRQVPLKALTRGQVQSALGGTVRQEIALQEG
                     IPTEKAREIVKLIKGTKRKVQAQIQENQVRVSGRDRDELQAVIKFLREQDLGIHMDFT
                     NYRS"
     gene            2854..>4361
                     /locus_tag="AC16G0_16020"
     CDS             2854..>4361
                     /locus_tag="AC16G0_16020"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding response regulator"
                     /protein_id="HIR5101161.1"
                     /translation="MIKRVLVVDNDRVCVEILGDILNQEGYEVVKAFDGMEAMEVLGR
                     GDLPDLVFLDIVMPRIDGDRVFEYMRAKPQTSRIPVVILSGTLMEDQTKILAMGADAY
                     VAKGPRDRLRRNVLATLQRLESGERAANPQVLGLENLVPHEKVRELLAIRQRRKAMLE
                     AIEQGIVELDGRGWVMAVNRAGLEILRRPELDLIGTPLAEILGPDYRETMEGVLTRFL
                     GGREKTTPVTLRYRGRVIEIRLAWNNPGDPTAGFFMLLQDVTDLAAKIEALTTLNAKL
                     KEMDRVRSEFLTMVSHDLHTPLTAIKGSLDVLLHEGVGPELTRELLGIAQKNADRLFR
                     LVSDILDLARIEEGRFSLQREPFDVVVTMRGTIDRLTHLAQDKGITLALQAPDGLSLV
                     MADGFRMEQVFTNLLGNALKFTPSGGRVAVTVKELATELLVEVRDSGVGIPTEHLDRV
                     FDRFYRVPLPAGTEVEGTGLGLSICKAVVEEHGGRIWVESVVGKGTAFFLTIP"
BASE COUNT          841 a         1303 c         1495 g          722 t
ORIGIN      
        1 ccgaagccgc cgaaggaagg caaggtccag caggcctttt ccttcctggc cgagaagggg
       61 gggccgatca acccctcgct gaagctcctg gatctcccct cggcggaacc ggtgggatca
      121 agccccgagg aggcggcgga gaattcgcgc atcctggaga agaagctcca ggagttcggg
      181 gtggagggtc gggtgacggc ggtgaacccg gggccggtca tcacgagcta cgagctcgag
      241 cctggaccgg gcatcaagat caaccgcatc gtggccctgg cggacgacct ggccctgggc
      301 ctgaaggctc tcagcgtccg cgtggttgct ccgatcccgg gcaaggcggc cgtgggggtg
      361 gagatcccca accagaatcg ggccacggtc cacctgcggg aggtcctggc cgccaaggag
      421 tttgccaacg agagcctgca cctgcccctg gcgctgggga aggagatcgg cggcgccccg
      481 gtggtggcgg acctgacgca gatgcctcac ctgctgattg ccggtgagac ggggtcgggg
      541 aaaagtgtgt gtatcaacgc cctgatcttg agcctcctgt accgatccca acctaagaac
      601 gtccggctgc tgctcatcga cccgaagcgg gtggagttgt ctgtgtacaa cggcatcccg
      661 cacctggcgg acaaggtggt gtcggacacc aaggatgccg cccggagact ccagcgcgtc
      721 gtggtgcaca tggaggagcg atacaagctc ttcgcccagg tgggagcacg gaatctccag
      781 agctacaacc gccgcatgga actggagccg ccgacccccg acgatctggc gcagggctcc
      841 taccgggggc agctccccat gctggtcgtg gtgatcgacg agctggcgga cctgatcatg
      901 gcggcccaaa gcgatgtgga gaacgccatc atgcgcctgg cgcagatggc acgggccgtg
      961 ggcatccacc tgatcgtggc cacccagcgc ccgtccgtgg acgtgctcac cggggtcatc
     1021 aaggccaact tccctgcgcg catctcgttc cgggtcgcgt ccaaggtgga ctcgcggacg
     1081 atcctggaca tgaacggcgc cgaggcgctc ctgggcaagg gggacatgct atacattcct
     1141 ccgggcagct ccaagccgat ccggatccac ggctgcaacg tgaccgaggt ggagatccgt
     1201 cgcgtcgtcg atttccttgc ggcgcagtcc aaggccccgg agtttgtctg gtctctcctg
     1261 ccgcccgagg aagaggcccc tgtcgaggat gaggaggggg atgagctgta ccgccaggcg
     1321 gtcgagatgg tggtgcagac ccagcaggcc tccatctcca tgatccagcg gcggctccgg
     1381 gtcggtttca acagggcggc gcgcatgatt gagcggatgg agcgggaggg catcgtgagc
     1441 cccatggacg gtacccgccc gcgcgaagtc ctggtgtcac gggcggagtc gtaggatgcg
     1501 gtacggcagc ttcatcccgg ccgtggccct gggggtcctc cttgaggtgg ggatcggatg
     1561 gcccgcgaat ggcccagcct ggctccctgg ggcgccggtg gccgaggccg cccccagcct
     1621 caacgaggtg gtggacaaag tccgcgagac atgcagccac gcccaggatc tttcggctct
     1681 atttgagcag atcaccacga tccgcagcct caatcaggaa cagagggcca gcggaattct
     1741 gttgctgaaa cggcccagaa agatgcgctg ggaataccag aagccggagt cgagactgtt
     1801 cgtgaccgac ggcaagacct tgtgggccta tagcccggcc gacaagcagg tggtggtgca
     1861 ggaggtgggc gaagccttcg cctcgcgcct tcccatttct atcctggccg gggactgtca
     1921 gctccgcagg gacttcgaca tccgcgaggt cgagaacgcc gccacccgag cgtccggcaa
     1981 tgtcatgatc ctggatcttc ggcccaagca atccgaggcc gggatcaccc gaatgctcct
     2041 cgaggtgaac ctcaagacct acacgatcga gcgaatcacg gtcttcgacg cctcggggaa
     2101 cacgagtgtg tacaagctgt cggatctgag gctgaatcct ggactcagcg accaacagtt
     2161 cacgttcacg cccccggcgg gcgtgacggt ggtgaacccg ccccaacagt gagggccgat
     2221 ggccgtgcgc aggccgcagg ccacgaccgg atggagacat ggccaaggag tgctcgttcg
     2281 acatcgcctc aaaggtggac atgcaggagg tggacaacgc cgttcatcag accacccgcg
     2341 agatcagcca gcgcttcgat ttcaagggga gtcaggcgga gatcacgcgg gaggggaaca
     2401 gcctcaagct cggcgccgag gatgagttca agctcaagca gatcctggac atcctggaga
     2461 cgaaactggt gaagcgccag gtgccgttga aggctcttac gcgcggccaa gtccagtccg
     2521 ccttaggcgg aacggtccgg caggagatcg cgctgcagga ggggatcccc acggagaagg
     2581 cccgggagat cgtcaagctg atcaagggga cgaaacgcaa ggtccaggcc cagattcagg
     2641 agaaccaggt ccgggtctcc ggccgcgacc gggatgaact ccaggctgtg atcaagttcc
     2701 tgcgggagca agacctgggg atccacatgg acttcacgaa ctatcggtcc tgatccacgg
     2761 aggggcagat tatacatcct ggttgagctg cccggatcca agagcgcgtc catggcgaac
     2821 tccgaccccc aaccccaaac ggcggagtct caagtgatca agcgagtctt ggttgtggac
     2881 aacgaccggg tctgcgtgga gattctgggc gatatcctga accaggaggg ctatgaggtt
     2941 gtcaaggcct tcgatggcat ggaggcgatg gaggttctgg gacgggggga cctgccggat
     3001 ctggtcttcc tcgacatcgt catgccacgc atcgacggcg atcgggtctt cgagtacatg
     3061 cgcgccaagc cgcagacgag tcgcatcccg gtcgtgatcc tttcgggcac cttgatggag
     3121 gaccagacca agatcctggc catgggggcc gacgcgtatg tagccaaggg gccacgcgac
     3181 aggcttcgtc ggaacgtcct ggcgaccctg cagcgcctgg aaagcggcga gcgcgcagcc
     3241 aacccgcagg ttttgggcct ggagaacctg gtcccacacg agaaggtaag agagttgctg
     3301 gcaatccggc agcgcaggaa ggcaatgttg gaggccatcg agcaagggat cgtggaatta
     3361 gacggccgcg gctgggtcat ggccgtcaac cgtgccgggc tggagatcct caggcggccg
     3421 gaactggatc tgattggaac gccgctggcc gagattctgg gtcccgatta tcgggagacg
     3481 atggaggggg tcctcacccg gttcctgggg ggccgcgaaa agacgacgcc ggtcacgctc
     3541 cgctaccggg ggcgggtgat tgagattcgc ctcgcctgga acaatccagg cgaccccacg
     3601 gccggattct tcatgctcct ccaggatgtc acggacctcg ccgcgaagat cgaggcactg
     3661 acgacgctga acgccaagtt gaaggagatg gaccgggtgc ggtccgagtt cttgacgatg
     3721 gtctcccacg acctgcatac gccgctcacg gcgatcaagg gatcgctgga tgtgctgctt
     3781 cacgagggcg tggggcccga actgacccgg gaattgctgg gcattgccca gaaaaacgcc
     3841 gatcggctct tccgcctggt gagcgacatc ctcgacctgg cacgcatcga ggagggccga
     3901 tttagcctgc agcgtgaacc ctttgacgtg gtggtcacga tgcgggggac gatcgatcgt
     3961 ctgacgcacc tggcccagga caaggggatc acgctggccc tgcaggcgcc cgacggactt
     4021 tcgttggtga tggccgatgg cttccggatg gagcaggtgt tcaccaacct tttgggaaat
     4081 gccctaaagt tcacgccctc ggggggccgg gtggctgtga cggtgaagga gctggccaca
     4141 gaactcttgg tcgaggtccg ggactccggc gtcggaatcc cgacggagca cctggatcgg
     4201 gtcttcgatc ggttctaccg ggtcccactc ccagcgggaa cggaggtgga aggaaccggc
     4261 ctcggtcttt ccatctgcaa ggcggttgtg gaggaacacg gcgggcgcat ttgggtcgag
     4321 agcgtggtcg ggaagggcac cgcctttttc ctgaccatcc c
//
LOCUS       DBNGZC010000928         4606 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_763275, whole genome shotgun sequence.
ACCESSION   DBNGZC010000928 DBNGZC010000000
VERSION     DBNGZC010000928.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4606)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_763275"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..1098
                     /locus_tag="AC16G0_16025"
     CDS             289..1098
                     /locus_tag="AC16G0_16025"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR5101162.1"
                     /translation="MPRSLALHTWTLDTTPLADVLRIARHTGWDAVELRRLDFARASE
                     SGQSAAEVLDLVRQSGLAVACVCVEAGWMFAAGPERRRLRRVFAESCEWAAQLQCPTV
                     MSPADRGRGDPQQAADNLREVGDLAAEHGVRLALEFNSQAEQFKTLNRVREVVTRAGH
                     PHCGLLVDTYHLYRSDADLRPLRDLAPDEIAYVQYSDVPGDGLRSGVTTDRLPPGQGI
                     VPFAEIFRLLEEKGYRGSLSYEAPNPSAWARNPEDVAREALAATRAVLPGA"
     gene            1230..2351
                     /locus_tag="AC16G0_16030"
     CDS             1230..2351
                     /locus_tag="AC16G0_16030"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009207387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR5101163.1"
                     /translation="MTKYIGILTSGGDNPGLNAAIRGVGKAAIGFHGMQVIGFLDGFR
                     GFMENRTMELDEDTLSGILTLGGTILGTSRDKPHRMPVGGKLLDMTDVMVDNYHRHNL
                     DVLVCLGGGGTQKNAYRLARKGLNIITLPKTIDNDVALTDVTFGFDTALSVATEAIDR
                     LHSTAHSHHRIIVVEIMGNKVGWLALGAGIAGGADVILIPEIPYDVECIAEAIRRRSH
                     TGKRFSLVAVAEGAMAKPEAEACRTAQQKAAKARSRTARTKAEADLKQLDAQHAGNTL
                     RLAKQLESLTGLESRVTILGYLQRGGTPSAADRLLATRLGTACADLIHRGQYGVMVAA
                     RGNGTKPVPLEDVARTLKSVPLDHTWVASARRVGTCLGD"
     gene            2384..2887
                     /gene="sixA"
                     /locus_tag="AC16G0_16035"
     CDS             2384..2887
                     /gene="sixA"
                     /locus_tag="AC16G0_16035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HIR5101164.1"
                     /translation="MELYILRHAIAVTRGTEGFPQDSDRPLTDKGRSKLRRVVRGMIA
                     LNLSFDQILTSPSVRARQTAEIVARDMGAAKKLESTPHLAPGGDPRALIRQLTSPRAP
                     GERILLVGHEPYLSELISVLVCGDEHTAITLKKAGLCKLAATTLRYGRCASLEWLLAP
                     SQMERIR"
     gene            2973..3854
                     /locus_tag="AC16G0_16040"
     CDS             2973..3854
                     /locus_tag="AC16G0_16040"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011142150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HIR5101165.1"
                     /translation="MKLEKFIRPFIVPAGKKISLKKDYSPSYTAKLVKEKDATEMLQR
                     GVQWLAEYQDRLYAQSTHALLIIFQAMDAAGKDGTIKHVMSGLNPQGCQVYSFKAPSA
                     EELDHDYNWRHFKALPERGRIGIFNRSYYEEVLAVRVHPEFLAAEKLPPSLNDEGIWN
                     RRFDEINQFERYLVHNGILVLKFFLNVSKAEQKKRFLERIDQPEKNWKFSAADAKERA
                     FWDDYMQAYQDVLNHTSTKWAPWYVIPADNKWFTRLAVAAVITAKLKQIDPQYPTVDK
                     ERKGEMRLARKMLEEEP"
     gene            complement(3863..>4606)
                     /locus_tag="AC16G0_16045"
     CDS             complement(3863..>4606)
                     /locus_tag="AC16G0_16045"
                     /inference="COORDINATES: protein motif:HMM:NF017085.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HIR5101166.1"
                     /translation="GILRDLDVFRLNLQGQSQRLAEGGAIAELLAEELGRQRGPMREA
                     LTSALGSRRFLTLMRRLEVLASSSSPRHPRGRQGVAVARGAPVLIRKAQKRVLKLGRT
                     IGPDSPAADLHRLRILFKRLRYACEFFQEAFVNPALGEDPLADYIQAMVRFQDCLGEH
                     QDAVVATARIQELAKDMVRRGGLAAERLLDLGALIQVQREIARERRGRLVKLWARFDK
                     RWVRKWLGGMEGRAPRGSRTGEASHTTPA"
BASE COUNT          870 a         1517 c         1454 g          765 t
ORIGIN      
        1 accggtcagc cttgctccgg cgccgccgcc ccagaggggc gcgtcgcgcc gtggagcgtg
       61 gatgtgtctt cagtttggag gaatcgcgcc acggcgtccc gaatccggct gacccgcggg
      121 cccggcgtgc tcggggcgcc gtcacaggcc tagattatgt tcgcggagtc gcaactcggg
      181 atactagcag gatgctgaac gacttgattt cgcccaggct gccgttcacg cattcgaggc
      241 gtggggcatc gtcatgcggg ctgaggggcc gcatccggga gaggtcttat gccgagaagc
      301 ctagcgctcc acacatggac actcgacaca acaccgctcg ccgacgtgct gcgcattgcc
      361 cgtcacaccg gctgggacgc cgtcgagctt cgtcgccttg actttgcccg ggcgtccgag
      421 tccgggcagt cggccgcaga ggtcctcgat ctcgtgcggc agagcgggct ggcggtcgcg
      481 tgcgtctgcg tcgaggccgg ctggatgttc gccgctggtc ctgagcggcg acgtctccgc
      541 cgggtcttcg ccgagtcctg cgaatgggcc gcccaacttc agtgccccac ggtcatgagc
      601 ccggcggatc gcggccgggg cgatccccag caagcggctg acaatctccg cgaggttggg
      661 gatctcgcgg cggagcatgg cgtgcgactt gccttggagt tcaactcgca ggccgagcag
      721 ttcaagacgc tcaaccgggt gcgggaagtg gtgacgcgtg ccggccaccc gcactgcggt
      781 ctattggtgg acacctacca cctctatcgc agcgacgctg acctccgccc cctccgagat
      841 ctggctcccg acgagatcgc ctatgtccag tacagcgacg tgcccggaga cggcctccgg
      901 tccggggtga cgacggatcg gctcccaccg ggtcaaggga tcgttccctt tgcggagatc
      961 ttccggctgc tggaggagaa gggctaccgc ggctccctga gctatgaggc gccgaaccca
     1021 tccgcctggg cgcgcaaccc cgaggacgtt gcccgcgagg ctctggctgc gacgcgcgca
     1081 gtgttgccgg gtgcttgacg cccaggcatc catctcattc ggtcgcgtct tgggtgtcca
     1141 ttccggccag cccaggaacg cggggttctt cgcatatcgg atacgctcgc atccatggga
     1201 agagtttgag acaaggaggc atctacgtcg tgaccaagta catcggcatc ctcacgtcag
     1261 ggggcgacaa ccccggcctg aacgcggcta ttcgcggggt gggaaaggcg gcgatcggct
     1321 tccatggcat gcaggtgatc ggcttcctcg acgggtttcg cggattcatg gagaaccgca
     1381 ccatggagct ggatgaagac accttgtcgg gcatcctgac cctgggcggc accatcctcg
     1441 gcaccagccg agacaaaccg catcgcatgc ccgtcggcgg aaaactccta gacatgaccg
     1501 atgtcatggt ggacaactac caccggcaca acctggatgt cctggtgtgt ctgggtggcg
     1561 gcggcaccca aaagaatgcg taccgcctgg ccaggaaggg gctgaacatc atcacgttgc
     1621 caaagaccat cgacaacgat gtggccttga ccgatgtcac cttcggcttt gacaccgcgc
     1681 tcagcgtcgc cacggaagcc atcgaccgct tgcacagcac ggcccacagt caccaccgca
     1741 tcatcgtcgt cgagatcatg ggcaacaagg ttggctggct ggcgctgggc gccgggatcg
     1801 ccggtggcgc cgacgtgatc ctcattccag agatccccta cgacgtggag tgcatcgccg
     1861 aagccatccg ccgccgcagc cacaccggca agcgattcag tctcgtcgcc gtggccgaag
     1921 gcgcgatggc gaaacccgag gcggaggcat gccggaccgc gcagcaaaag gccgccaagg
     1981 ccagatccag gactgccagg accaaggccg aggcggacct caagcaattg gacgcccagc
     2041 atgccggcaa caccttgcgg ctggcaaaac aactggaaag tctgaccggc ctcgagtcgc
     2101 gcgtcaccat cctgggctac ctgcagcggg gcggcacgcc ctccgcggcc gatcggctgc
     2161 tggccacccg tctgggcact gcctgcgccg acctcatcca ccggggccaa tacggtgtga
     2221 tggtggcggc gcggggcaat ggcaccaagc ccgtgccgct cgaagacgtc gcccggacac
     2281 tgaagtcagt tcccctggat cacacctggg tcgccagtgc gcgccgcgtg ggaacgtgcc
     2341 tgggcgacta accgacaggc cgtaataccc atcctgaggt cccatggaac tctacattct
     2401 tcgccacgcc atagcggtga cgcggggtac cgagggcttt ccgcaggatt ctgacaggcc
     2461 gctgaccgac aagggccgct ccaaattacg tcgggtcgtg cgggggatga tcgccctcaa
     2521 cctatccttc gatcagatcc tgaccagtcc ttctgtccgt gcgcggcaaa ccgccgagat
     2581 cgtcgccaga gacatgggtg ctgccaagaa gctcgagtcg accccgcatc tggctccagg
     2641 tggcgacccg cgcgcgctga tccgtcagct cacatccccg cgcgcccccg gggagcgcat
     2701 cctcctggtc ggtcacgagc cctaccttag cgaattgatc tccgtcctcg tatgcgggga
     2761 cgagcacacc gccatcacgc tgaaaaaggc tgggctgtgc aagctggcag ccacgacgct
     2821 acgctatggc aggtgcgcaa gtctcgagtg gctgcttgct cccagccaga tggaaagaat
     2881 ccggtagact catcatcccc ttgccgccgc ccccggtttg tgagcattca tcacctttcg
     2941 atctcgcggg cgcagactag gagacagaat ccatgaaact cgaaaagttc attcgccctt
     3001 tcatcgtccc ggcaggcaag aagatctcgt tgaagaaaga ctatagcccc agctacaccg
     3061 ccaagctcgt gaaagagaag gacgccaccg agatgctgca gcggggagtg cagtggctgg
     3121 cggagtacca ggacagactg tatgcccaga gcacccacgc cctgctgatc atattccagg
     3181 ccatggatgc cgccggcaag gatgggacga tcaagcacgt catgtccggc ctgaatccgc
     3241 aaggctgcca ggtgtacagc ttcaaggccc cgtctgccga ggaactcgat cacgactaca
     3301 actggcgcca cttcaaggcc ctgccggagc gcggacgcat cggcatcttc aaccgctcct
     3361 actatgagga ggtccttgcg gtgcgcgtcc atccggaatt cctggcagcc gagaagctgc
     3421 ccccctcctt gaacgacgag gggatctgga accgccgctt cgacgagatc aatcagttcg
     3481 agcggtacct ggtccacaac ggcatcctgg tgctgaagtt cttcctgaat gtgtcgaagg
     3541 cggagcagaa gaagcgcttc ctggagcgga tcgatcaacc cgagaagaac tggaagttct
     3601 cggcggccga tgcgaaggag cgcgcattct gggatgacta catgcaggcc tatcaggacg
     3661 tgctcaacca cacgagcacg aagtgggcgc catggtatgt catcccggcg gataacaaat
     3721 ggtttacccg cctggcggtc gcggccgtca tcacggccaa gctcaaacag attgatccgc
     3781 aatacccgac ggtggacaaa gagcgcaagg gggaaatgcg cctggccagg aagatgctcg
     3841 aagaggagcc gtgaccggcc gtctaggcag gagttgtgtg cgatgcttcg ccggttctcg
     3901 accctcgcgg cgccctcccc tccatacccc cgagccactt ccgcacccag cgcttatcga
     3961 accgggccca gagcttgacg aggcgccccc ggcgctcgcg tgcaatctcc cgctgcacct
     4021 ggatgagagc ccccagatcc agaagacgct ccgcggcgag cccgccacgc cggaccatgt
     4081 ccttggccag ttcctggatc cgggccgtgg ccacgacggc gtcctggtgc tctcccaggc
     4141 agtcctggaa ccggaccatc gcctggatgt aatccgcgag ggggtcctcg ccgagcgctg
     4201 ggttcacgaa agcctcctgg aagaactcgc aggcgtacct gagtcgctta aacaggattc
     4261 tcagtcggtg caggtctgcg gcgggcgagt cggggccgat ggtccgtccc agcttcagga
     4321 cccgcttctg ggccttacgg atgagcaccg gcgccccccg ggccacggca acgccttggc
     4381 ggccgcgggg atggcgggga gaagacgagg acgccaggac ttccagacgg cgcatgagag
     4441 tgaggaagcg tcgggagccc aaggcggagg tgagagcctc acgcatgggc ccacgctgcc
     4501 gacccaactc ctccgccagg agttccgcga tggctccccc ttccgccaat ctctgagact
     4561 gcccctggag attcagcctg aagacgtcca ggtcccgaag aatccc
//
LOCUS       DBNGZC010000929         2770 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1642875, whole genome shotgun sequence.
ACCESSION   DBNGZC010000929 DBNGZC010000000
VERSION     DBNGZC010000929.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1642875"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..1560)
                     /gene="hslU"
                     /locus_tag="AC16G0_16050"
     CDS             complement(211..1560)
                     /gene="hslU"
                     /locus_tag="AC16G0_16050"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HIR5101167.1"
                     /translation="MSVDRLTPRQIVAELDKYIIGQGDAKRAVAIALRNRWRRTRLPA
                     ALRDEVAPKNIIMIGPTGVGKTEIARRLARLAEAPFIKVEASKYTEVGYVGRDVESMI
                     RDLVELAVNMVRTEKTRAVEGRAKELAEERLLDLLLPSSRPGGFGQVVAPETGPGHET
                     REKLRQRLREGSLDERLVEMEVRDRALPMMEVLGGQGMEEVQENLKEMLGNLLPQRTK
                     RRRVKVSEARRILTQEEAEKLVEKEEAVSEAIRRVEDSGIVFLDEIDKIAGREHAQGP
                     DVSREGVQRDLLPIVEGCTVSTKHGLVRTDHILFIAAGAFHVSKPSDLIPEMQGRFPL
                     RVELQSLTRDDFVRILTEPENALTKQYSALLATEEVDLSFTRDALEEIASIATEVNQS
                     TENIGARRLYTIMERLLDEISFEAPHMSGMKVEIDAQQVRKKLASIVKDQDLSRYIL"
     gene            complement(1667..2245)
                     /gene="hslV"
                     /locus_tag="AC16G0_16055"
     CDS             complement(1667..2245)
                     /gene="hslV"
                     /locus_tag="AC16G0_16055"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HIR5101168.1"
                     /translation="MTTRANHSGREVEGRSPSVRSTTIVSVRHKGRVAIAGDGQVSFG
                     EVVMKHTARKVRRMYNDTVIAGFAGAAADAFALFAKFEAKLEEFNGNLPRAAVELAKD
                     WRLDRALRRLEALLAVVDREHSLILSGTGDVIEPDDGIIGIGSGGGYATAAARALVRH
                     SSLSAREIVEEAMHIAAAICVYTNDQITVEEL"
     gene            complement(2242..>2770)
                     /locus_tag="AC16G0_16060"
     CDS             complement(2242..>2770)
                     /locus_tag="AC16G0_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR5101169.1"
                     /translation="LFYASGLRIGELVGLNRSDVDLQEGLARVLGKGRKERIVPVGKK
                     AIEALNAYLVERDALVAGSRDRTEGPQALFLNHRGGRLTARSVSRTVLWHLLASGLGK
                     KVTPHGLRHSFATHLLTAGADLRAIQELLGHARLSTTQRYTHVGLGEVMKVYDETHPR
                     AKLGSKTRRPVPGTA"
BASE COUNT          418 a         1006 c          844 g          502 t
ORIGIN      
        1 cggccgtccc ttccgccagg accgtctggg cctgggtggc gttctccgcc accttcacgc
       61 caaacccagc cttctccagc tcgtcccgga cgagggtcag gaacagccga tcgtcatcca
      121 caaccaggat cgtttccatg acgcatcacc cagccagcta cgaggagagg gggtgaatct
      181 cgtggcctcc gattgatccg cctccaaccg ctacaaaatg tagcggctga ggtcttggtc
      241 cttgacgatg ctcgccagct tcttccgcac ctgctgggcg tcgatctcca ccttcatccc
      301 agacatgtgc ggagcctcga aagagatctc atccagcagg cgctccatga tggtgtagag
      361 acggcgcgcc ccgatgttct cggtggactg gttgacctcg gtggcgatag aggcgatctc
      421 ctccagggcg tcccgcgtga aggacaggtc cacctcctcc gtcgccagga gggccgagta
      481 ctgcttggtc agggcgttct ccggctccgt caggatgcgc acgaagtcgt cccgggtcag
      541 gctctgcagc tcgacgcgaa gcgggaaccg gccctgcatc tcagggatga ggtccgaggg
      601 cttggagacg tggaaggcgc cggcggcgat gaacaggatg tgatccgtcc ggacgagccc
      661 gtgcttggtg ctgaccgtgc acccctccac gatgggcaga aggtcccgct gcacgccctc
      721 gcgggagacg tccggcccct gggcgtgctc gcgtcctgcg atcttgtcga tctcgtccag
      781 gaacacgatg cccgaatcct cgacgcggcg gatggcctcg ctgaccgcct cttccttctc
      841 caccagcttc tccgcctcct cttgcgtgag gatgcggcgc gcctcgctca ccttgaccct
      901 gcggcgcttc gtgcgctgcg gcagcaggtt tcccagcatc tccttgagat tctcctgcac
      961 ctcctccatg ccctggccgc ccagcacctc catcatgggc agggcccggt cccggacctc
     1021 catctcgacc aagcgctcgt ccagggaccc ttcccgcagg cgctggcgca gcttctcccg
     1081 ggtctcgtgg cccggccccg tctcgggggc cacgacctgg ccgaaccccc ccggccggga
     1141 cgagggcaac agcaggtcca gcaggcgctc ctcggccagc tccttcgccc ggccctccac
     1201 ggccctggtc ttctcggtcc ggaccatgtt caccgccagc tccaccaggt cgcggatcat
     1261 ggactcgacg tcccgcccga cgtagcccac ctcggtgtac ttggacgcct cgaccttgat
     1321 gaagggagcc tcggcgagtc gcgccagcct gcgggcgatc tcggtcttcc ccacgccagt
     1381 cgggccgatc atgatgatgt tcttcggcgc cacctcgtcc cggagggcgg ccggcagcct
     1441 ggtcctgcgc cagcggttcc ggagcgcgat ggccacggcc cgcttcgcgt ccccttggcc
     1501 gatgatgtat ttgtccagct ccgccacgat ctggcggggt gtcagccgat ccacgctcat
     1561 agtatagccc tcgcggaggc gcggaggcgc acgggcgcag aggggaaata gcttccgccc
     1621 cagcctcgcc tctgcgcctt ttcgcccctg cggctctgca gcatcctcaa agctcctcca
     1681 ccgtgatctg gtcgttggtg tagacgcaga tcgcggcggc aatatgcatg gcctcttcca
     1741 cgatctcccg ggccgagagg ctcgaatgcc ggaccagagc gcgtgccgcg gccgtggcgt
     1801 agcctccacc ggacccgatg ccgatgatcc cgtcgtccgg ctcgatcacg tcgccggtcc
     1861 cggagaggat cagggagtgc tctcggtcca ccacggccag caaggcctcg agtcgccgca
     1921 gggcccggtc gagtctccaa tccttcgcca gctccacggc ggcgcgggga aggttcccgt
     1981 tgaattcttc cagcttggcc tcgaacttcg caaacagcgc gaaggcgtcc gctgccgcgc
     2041 cggcgaaccc ggcgatcacc gtgtcgttgt acatccgccg gaccttgcgg gccgtgtgct
     2101 tcatcaccac ctcgccgaag gagacctggc cgtctccggc gatcgccacc cggcccttgt
     2161 gtcgaacaga cacgatggtc gtgctgcgca cgcttgggga ccgcccctcc acttccctgc
     2221 cggaatgatt ggcccgggtc gtcatgccgt ccccggaacc gggcgtcgcg tcttagagcc
     2281 gagtttcgcc cgcggatggg tctcgtcgta caccttcatg acctccccca gacccacatg
     2341 ggtgtagcgt tgggtcgtgc tcaatctggc gtgccccagg agttcctgga tggcccggag
     2401 atccgcgccg gcggtcagta ggtgggtggc gaagctgtgg cgcaggccgt ggggcgtgac
     2461 cttctttccc agaccgctcg ccagcagatg ccagaggact gttcgggaca cgctccgggc
     2521 cgtgaggcgc cctccgcgat ggttcaagaa aagagcctga ggcccctccg tacgatctct
     2581 gcttccggcc accagagcat cgcgctcgac gagataggca ttgagggcct cgatagcctt
     2641 cttccccacc ggaacgatcc gctccttccg gcccttgccc aggacccgcg ccaacccctc
     2701 ctggagatcc acgtccgacc ggttcagtcc caccagctcc ccgattcgaa gacccgaagc
     2761 gtaaaacagc
//
LOCUS       DBNGZC010000930         5195 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2720207, whole genome shotgun sequence.
ACCESSION   DBNGZC010000930 DBNGZC010000000
VERSION     DBNGZC010000930.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2720207"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..769
                     /gene="lipB"
                     /locus_tag="AC16G0_16065"
     CDS             74..769
                     /gene="lipB"
                     /locus_tag="AC16G0_16065"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIR5101170.1"
                     /translation="MGAPAAETEARARVAWQVLDLDLQPYALTLHQQEGLVGQRIAGC
                     IPDSLILVEHPPVVTLGRAKHRRNLCVGPEELAARGIEFFEITRGGDVTYHAPGQLVG
                     YPIFDLKQHGRDVLRFCRGVESALVAALGDFGLAARAIPGDAGVWVGEKKIASLGISV
                     RRWVTFHGFALNVSTDLTGFRVIRPCGKDPEVMTSMAALLGRPVPMEEVRRRVTARFA
                     EIFRFDETLGVSA"
     gene            802..2046
                     /locus_tag="AC16G0_16070"
     CDS             802..2046
                     /locus_tag="AC16G0_16070"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF052515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor-dependent isomerase,
                     group 22"
                     /protein_id="HIR5101171.1"
                     /translation="MVFPRILRVKQQLEGPTLRDIPRAVRESVQALHLADRVKPGQTV
                     AITSGSRGVANIDRITRTVVDEMKALGLKPFIVPTMGSHGEAKAEGQVKVLEHYGVTE
                     AAMGCPIKSSMAVVEIGKVKGTTVFCDKNAWEADHIAVVGRIKAHTDFNNDIESGLFK
                     MMAIGLGKQHGAENYHRAGHHYSYAEIFPLVGKAVLETGHVLLGLGIVENGYDETAKV
                     MALLPQDFEAQEKALLKEAKVWMARLPFDQIDLLIVDELGKNISGAGMDPNVIGRASV
                     QKPAGKPHVRNLFVRDLRPESEGNAIGVGFADMTTWRLVKKINYAAMYMNAITSGIAE
                     AAKVPMAFDSDRDAVQTALGMIGLTAPEQARVVRIKNTLHLTEIDVSESMLSEVKANA
                     RLSQVTDPASLTFDGSGTLPPF"
     gene            2175..2504
                     /locus_tag="AC16G0_16075"
     CDS             2175..2504
                     /locus_tag="AC16G0_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5101172.1"
                     /translation="MAGYFIDPKELKAKEIAPGTQIKVAYGEKLMFSFVDVKPGSVVP
                     MHSHPHEQGGICLEGALEFTIGSETRIVRKGEAWLIPGGVPHGVRGLQSGAYVLDIFY
                     PHREEYK"
     gene            2615..3385
                     /locus_tag="AC16G0_16080"
     CDS             2615..3385
                     /locus_tag="AC16G0_16080"
                     /EC_number="4.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase family protein"
                     /protein_id="HIR5101173.1"
                     /translation="MSRGQVVWGTFVFEFASASVPRILKEAGWDYILIDTEHASFGID
                     TVSTLLHVSSSIDLPALVRVPETQRSLLSRPLDAGAMGLMIPRVESRAQAEAIVRYTK
                     FPPTGDRGVVLGTAQNAYRPVDGRRFVRQANAELLLIMQIETLDGLEHMDQILSVPGL
                     DVALIGPYDLSTSMGIPGEMNHPRMRHAIEDFLKSCRRHGVVPGNFVTTVEDGRAWLR
                     RGMRFLIYSVDFLLVMERSQQVLSQLRPKRRTDDRRPR"
     gene            3366..4382
                     /locus_tag="AC16G0_16085"
     CDS             3366..4382
                     /locus_tag="AC16G0_16085"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0003714 - transcription corepressor
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_function: GO:0140297 - DNA-binding transcription factor
                     binding [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HIR5101174.1"
                     /translation="MTGAPARFRVVFTDPHRFPIGVERDVLSRVDCDLVPATCHNEED
                     TVTACRDAHAILTSTARFSARVIAELRQALVICRYGIGVDTVDVPAATEHGIVVANVP
                     DFCFDQAGDTAMSLILSVPRKVTYINNLIRKGVYNRDLARPIHNFRGASLGFVAFGNV
                     ARNVARKAVPFGFRLLAYDPYLKPDDVLDYPVTLVDFETLLRESDFISVHAPLTPETR
                     HLFDETAFRTMKPTAYFFNLGRGPVHDQHALYRAVKEGWIAGAGLDVLEQEPPDPTDP
                     ILTLDHVVFTPHYASYTEEAYHELRVKTAENAAAVLEGRFPKYVYNPEVKARARFLQH
                     GNPQ"
     gene            4414..4932
                     /locus_tag="AC16G0_16090"
     CDS             4414..4932
                     /locus_tag="AC16G0_16090"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HIR5101175.1"
                     /translation="MRLKGKKVAVLAENMYQEMELWVPYFRLKEEGADVKIVGPEKAT
                     YTSKHGYPVTADVAAKDVSAKDFDGVVIPGGYAPDMLRRHKPILDLVKGCFDQGKPVA
                     AICHAGWVPASAGILKGKTATCFFAIKDDVINAGAKYVDQEVVVDGNLVTSRTPNDLP
                     AFCRELVKALEK"
BASE COUNT          967 a         1620 c         1731 g          877 t
ORIGIN      
        1 cggtttggtc atcacgcgcg acgattggcg gatgggtcgg ctattggagc gagcaatggg
       61 cgtgaccatt gcgatggggg cccccgcggc cgagaccgag gcgcgcgctc gggtggcctg
      121 gcaggtcctg gacctggatc tccagccgta cgccctgacg ctgcatcagc aggaagggct
      181 ggtgggtcag cgcatcgcgg gatgcatccc ggacagcctc atcctggtcg agcacccacc
      241 cgtggtcacc ttgggccgcg ccaagcatcg gcggaatctt tgcgtcggcc cggaggagct
      301 ggcggctcgg gggatcgagt tcttcgagat cacgcgcggc ggtgacgtga cataccatgc
      361 ccccggccag ctcgtgggct acccgatctt cgatctcaaa cagcacggtc gggacgtgct
      421 ccgcttctgc cggggggtcg agtccgccct ggtcgcggcc ctgggagact tcggcctggc
      481 ggcgagggcg atacccggag acgccggcgt gtgggtgggg gagaagaaga tcgcctctct
      541 ggggatctcc gtgcgtcgct gggtgacgtt ccacggtttt gccctgaacg tctccaccga
      601 cctcaccggt tttcgggtga tccggccgtg cggcaaggat ccggaggtga tgacttccat
      661 ggccgcgctc ctgggacggc cggtgcccat ggaggaggtc cggcggaggg tgacggctcg
      721 atttgccgag atcttccgct tcgacgagac ccttggcgtt tccgcatgat cgccagatga
      781 tcgaatgaca ggaggctcag catggtattt ccgcgcattc ttcgagtgaa gcagcaactg
      841 gaaggaccga cgctccgaga catccccagg gccgtccgcg agtccgttca agcactgcac
      901 ctggccgatc gggtcaagcc gggacagacg gtggcgatca cctcgggcag tcggggagtc
      961 gccaatatcg atcggatcac ccggaccgtg gtggacgaga tgaaggcgct cggcctcaag
     1021 cctttcatcg ttccgaccat gggaagtcac ggcgaggcca aggccgaggg gcaggtgaag
     1081 gtcctggagc actacggggt caccgaggcc gccatgggct gcccgatcaa gtccagcatg
     1141 gcggtggtcg aaatcgggaa ggtcaagggc accacggtct tttgcgacaa gaacgcctgg
     1201 gaggcggacc acatcgccgt ggtggggcgg atcaaggctc acaccgactt caacaacgac
     1261 atcgagagcg gcctgttcaa gatgatggcc atcggtctgg gcaagcagca cggggcggag
     1321 aactatcatc gcgcgggcca tcactacagc tacgccgaga tcttccccct ggtgggcaag
     1381 gccgtcctgg agacgggcca tgtccttctc gggctgggga ttgtggagaa cggctacgac
     1441 gagacggcca aggtcatggc cctgcttccc caggacttcg aagcgcagga gaaggccctg
     1501 ctgaaagagg ccaaggtctg gatggcccga cttcccttcg accagatcga cctgctcatc
     1561 gtggatgagc tcgggaagaa catcagcggc gccggcatgg atcccaacgt catcgggcgc
     1621 gcctcggtcc agaagccggc ggggaagccg cacgtccgca acctcttcgt tcgcgatctt
     1681 cgcccggaga gcgaggggaa cgccatcggg gtcggcttcg ccgacatgac cacctggcgc
     1741 ctggtgaaga agatcaacta cgcggccatg tacatgaacg ccatcacgtc aggcatcgcg
     1801 gaggcggcca aggtgccgat ggccttcgac tccgaccggg acgccgtcca gacggcgctg
     1861 gggatgatcg ggctgaccgc gccagagcag gcgcgggtcg tgcggatcaa gaacacgctc
     1921 cacctgaccg agatcgacgt ctccgagtcc atgctgtccg aggtgaaggc gaacgcgcgc
     1981 ctgagccagg tcaccgatcc ggcgagcctg acattcgacg ggtcggggac tctgccgccc
     2041 ttctaaagca aatgcccaat gaccaatgac aaatgaccaa tgtgagaacc ctctggatcg
     2101 accgcaatac ccacattgat cggtcgggtc acccattggt cattgggaat tggtcatgag
     2161 atccggaggg tatcatggct ggatacttca ttgacccgaa ggaattgaag gcgaaggaga
     2221 tcgcgccggg gacccagatc aaggtcgcct acggcgagaa gctcatgttc tccttcgtgg
     2281 acgtcaagcc gggctcggtg gtccccatgc acagccatcc ccacgaacag ggcgggatct
     2341 gcctggaagg ggcgctggaa ttcaccatcg gcagtgagac gcgaatcgtg cgcaagggcg
     2401 aggcgtggtt gatccccggg ggggtacccc acggcgtgcg ggggctccag tctggtgcgt
     2461 acgtcctgga tatcttctat cctcaccgcg aggagtacaa gtagagagct gagagctgag
     2521 agccgagagt tgcgcgccgc ggccgcgcgc cgggtagagg cctgcgcgcg acggacgaaa
     2581 gaggtggggc attcgaaatc cgctgaaggc caagctgtcg aggggacaag tcgtctgggg
     2641 gacctttgtc ttcgaattcg cctcggcttc ggtaccccgg atcctcaagg aagcgggctg
     2701 ggactacatc ctgatcgaca ccgagcacgc gagcttcggc atcgacacgg tttccacctt
     2761 gctccacgtg tcctccagca tcgacctgcc ggccttggtg cgggttcccg agacccagcg
     2821 ttccctcctg agccgcccgc tggacgccgg cgccatgggt ctcatgatcc cccgcgtgga
     2881 aagccgtgcc caggccgagg ccatcgtccg gtacacgaag tttccgccga cgggtgaccg
     2941 cggggtcgtc ctcggcaccg cccagaatgc ctaccggcca gtggacggcc ggcggtttgt
     3001 gcgtcaggca aacgcagagc ttctgctcat catgcagatc gaaaccctgg acgggctgga
     3061 gcacatggac cagatcctgt cggtgccggg cctcgacgtg gcgctgatcg gtccctatga
     3121 tctgtcgacc tccatgggca ttccgggcga gatgaatcat ccgcgtatgc ggcacgccat
     3181 cgaggacttc ctcaagagct gccggcggca cggggtcgtg ccgggcaact tcgtgaccac
     3241 ggtggaggac ggacgggcgt ggctccggcg ggggatgcgc ttcctgatct acagcgtgga
     3301 tttcctcttg gtcatggaac gcagccagca ggtcttgagc caactgcgtc ccaagcggag
     3361 gaccgatgac aggcgccccc gctaggtttc gagtcgtgtt cacagacccc cacaggtttc
     3421 ccatcggcgt cgagcgggac gtcttgagcc gggtggattg cgatctggtg ccggcaacct
     3481 gccacaacga ggaggacacc gtcacggcct gccgcgacgc ccacgccatc ctcacctcca
     3541 ccgctcggtt cagcgcccgg gtgattgccg agctgaggca ggccttggtc atctgccgct
     3601 acggcatcgg ggtggacacg gtggacgtcc cggcggctac cgagcacggc atcgtcgtgg
     3661 ccaacgtgcc cgatttctgc ttcgaccagg ccggcgatac ggccatgagc ctcatcctct
     3721 cggtcccgcg caaggtgacc tacatcaaca acctgatccg gaagggagtg tacaaccggg
     3781 atctggcccg accgatccac aactttcgcg gcgccagcct gggttttgtg gccttcggga
     3841 acgtcgcccg gaatgtggcg cgcaaggccg tccccttcgg gttccggctg ctggcgtatg
     3901 acccctacct gaagcccgac gatgtgctcg attatccggt gaccctggtg gacttcgaga
     3961 ccctgctccg ggaatcggac ttcatctcgg tccacgcgcc cctgacgccg gagactcgcc
     4021 atctcttcga cgagaccgcc ttccgcacga tgaagcccac ggcctatttc ttcaacctcg
     4081 ggcgcggccc ggttcacgat caacatgccc tgtaccgggc cgtaaaggag gggtggatcg
     4141 ccggggcggg tctggacgtt ctggagcagg agccgcccga tccgaccgat cccatcctca
     4201 ccctggatca cgtcgtcttc acgccccact acgcctccta caccgaagag gcgtaccacg
     4261 agctgcgggt gaagacggcg gaaaacgcgg ccgctgtcct ggagggacga tttcccaaat
     4321 acgtgtacaa tcccgaggtg aaagccaggg cgcggtttct gcagcacggc aacccccagt
     4381 aaccatagcc acaggacgga ggaggagcga accatgcgac tcaagggaaa gaaagtagca
     4441 gtgctggccg agaacatgta ccaggagatg gagctgtggg tcccctattt tcgactgaag
     4501 gaggaaggag ccgacgtcaa gatcgtgggg ccggagaagg caacctacac cagcaagcac
     4561 ggctatcccg tcaccgccga cgtggcggcc aaggatgtgt ctgccaaaga cttcgacggg
     4621 gtcgtcatcc ctggcggcta cgccccggac atgctgcggc gccacaagcc gatcctggac
     4681 ctggtcaagg gctgcttcga ccagggcaag ccggtggcgg ccatctgcca cgcgggctgg
     4741 gtacccgcct ccgcagggat cctgaagggg aagacggcca cctgtttctt cgccatcaag
     4801 gatgacgtga tcaacgccgg cgccaagtac gtggatcagg aggtcgtggt ggacggcaac
     4861 ctggtcacct cgcgcacgcc gaacgatctc ccggccttct gccgcgagct ggtgaaggcc
     4921 ttggaaaaat aggagaagac ggtgctgcgg ttcggggtgc tgcggttcgc ggtgagagaa
     4981 cggaccgcag tacctcgaac cttgcactgc agcaccgcgc aggagcgctc atgattctcc
     5041 ccaagctgta tcgcatccga caaaagctcg atccgccggt catcgccgac gtggcggccg
     5101 cagtccgggc cgagattggc aagctggatc tcgcgggtcg cctgaagcct ggcgcgcggg
     5161 tggccgtgac cgggggcagc cgcggcgtgg cgaac
//
LOCUS       DBNGZC010000931         3532 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1545808, whole genome shotgun sequence.
ACCESSION   DBNGZC010000931 DBNGZC010000000
VERSION     DBNGZC010000931.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1545808"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1921
                     /locus_tag="AC16G0_16095"
     CDS             <1..1921
                     /locus_tag="AC16G0_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019999575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HIR5101176.1"
                     /translation="IATALGDRIRIRGVGTTGSGRELIGELVGADTITDEITAHTTGA
                     TYVSKTLLGEQVDTIFEIGGQDSKFIALEDGVVVDFAMNEACAAGTGSFWEEQAERLG
                     ISIKGEFSDLAFRSRNPVRLGERCTVFMEQEVLAWQQRGAAREDLVAGLAYSVALNYL
                     NRVVRGRRIGVGIYFQGGTAYNDAVAAAFAGLLGKRIIVPPHNGVMGAIGMALLAREK
                     VQGTGRSTTFRGFDLSTVRHSLREFTCKACSNLCDMQEFTVEGVKTYWGDKCSDKFRT
                     RRRTDRRPVLPDLMALRRARLLEGYEGPQGAGPRVGVPRAISFHDRFPFWRAFLEGLG
                     CAVVISDETNRKIAQDGIDLSVAEPCYPIRIAHGHVQDVLDKGVDWILLPNTVNAEPT
                     PDAVESQLCPWVQTLPFVVRSVPRLRAVRDRILCPTVHFRRGEAHVAQELVPLGRALG
                     ADVVQTHRAVACGFAAQRAFGAALEGLGREALDTLQAAGEPAIVLVGRPYNVHDRAIN
                     LDIPGKLRDYYGINVIPMDCLALAALDIRDVNDNMYWHSGRRILAAGKLVARFPNLHL
                     IHLTNFKCGPDSYLKHFLRTANGKPYLSLQFDGHSNDAGFLTRCEAYLDSQGMLRWWR
                     KPIQVSGDDGCTSSA"
     gene            1918..3144
                     /locus_tag="AC16G0_16100"
     CDS             1918..3144
                     /locus_tag="AC16G0_16100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101177.1"
                     /translation="MTDCGTLALAAAFRSAGIDAEVLPPPDARTLLLGARHLTGEECL
                     PAKVTLGDYLKVAEAPDFDPRRTAFMMPTTEGPCRFGQYAPFIRKVFGDLGYPEVVIF
                     SPTSGDGYQTTEGVGASLPRLSWRALVTSDILRKLLLKTRPYERTPGDADAVYHDSLQ
                     TLCAALERRGLGPRAHLQLLIETLSGIRDRFRTLPARYERGRLLIGVQGEIFCRMEEF
                     SNDQLIRHLEACGGEAWLSDLGEWVWYCNNDQELCLRRDGRRVSFAMLGAKLRDRIQR
                     ADEAALRAPCQEDFGGYEDPEDIEIILRAAMPYLPQSGASGEMVLSAGKVDYLFRRGV
                     DGIIDISPFSCMNGIVSEALYPRQSQEHAGLPIRNFYFDGKGRDLTSDLEIFLELARA
                     YQQQKPYPRRYPPCFT"
BASE COUNT          608 a         1115 c         1166 g          643 t
ORIGIN      
        1 gatcgcgacc gcccttggcg accgcatccg aatccggggc gtgggcacca cgggctcggg
       61 acgggagttg atcggggagc tggtgggcgc cgacaccatc accgacgaga tcaccgcgca
      121 caccaccggc gccacgtacg tgagcaagac tctcttgggc gagcaggtgg acaccatatt
      181 cgagatcggg ggacaggact ccaagttcat cgccttggag gatggcgtgg tggtggattt
      241 cgccatgaac gaggcctgtg cggcggggac gggctcgttt tgggaggagc aggcggagcg
      301 cttgggcatt tcgatcaagg gggagttctc ggatctcgcc ttccggtccc ggaatcccgt
      361 ccgcctgggc gagcggtgca ccgtgttcat ggagcaggag gtcctcgcct ggcagcagcg
      421 tggggcggcg cgcgaagacc tggtcgccgg actggcgtac tcggtggccc tcaactacct
      481 gaaccgggtg gtccggggtc ggcgcatcgg ggtggggatt tactttcagg ggggcacggc
      541 ctacaacgat gccgtggccg cggcctttgc cgggttgctc ggcaaacgca taatcgtgcc
      601 cccgcacaac ggggtgatgg gggccatcgg catggccctg ctggcgcgcg agaaggtgca
      661 ggggacgggc cgctccacca cgttccgcgg attcgatctc tcgacagtcc gccacagcct
      721 gcgggagttc acgtgcaagg cttgctcgaa cctttgcgac atgcaggagt tcacggtcga
      781 aggggtcaag acctactggg gtgacaagtg cagcgacaag ttccgtacac gccggcggac
      841 ggaccggcgg cctgtgctcc ccgacctcat ggctctccgc cgggcgcgac tgctggaggg
      901 ctatgagggg ccgcaaggag cgggcccccg ggtcggcgtg ccgcgagcca tctctttcca
      961 cgatcgcttc cccttctggc gggccttcct ggaagggctt ggctgcgctg tggtcatctc
     1021 ggatgagacg aatcggaaga tcgcccagga cggcatcgac ctcagcgtgg cggagccctg
     1081 ctaccccatc cggatcgccc acggccatgt gcaagacgtc ttggacaagg gagtggactg
     1141 gatcctcctc ccgaatactg tgaatgccga gccgacgccc gacgcggtgg agtcccagct
     1201 ctgcccttgg gtccagaccc tgccctttgt ggtgcgaagc gtgccgcgcc tccgggccgt
     1261 tcgggaccgg atcctctgtc ccaccgtgca ctttcgacgg ggcgaggcgc acgtggctca
     1321 ggaactcgtt cccttgggtc gcgcgctggg tgcagatgtc gtccagacgc accgggcggt
     1381 ggcctgcggg ttcgccgccc aacgggcctt cggggcagcc ctggaaggcc tgggccggga
     1441 ggccctggac acgcttcagg ccgccggtga gccggcgatc gtcctggtag gccgcccgta
     1501 caatgttcac gatcgggcaa tcaatctgga tatccccgga aagctgcggg actactacgg
     1561 catcaacgtg atccccatgg attgtttggc cctcgccgca ctggacatcc gggatgtgaa
     1621 cgacaacatg tactggcatt ccgggcggcg catcctggcc gcgggaaagc tcgtggcgcg
     1681 cttcccgaac ctgcacctca tccatctcac gaatttcaag tgcggcccgg actcatacct
     1741 caagcacttt ctgagaacgg ccaatggaaa accgtatctc agcctgcagt tcgacggcca
     1801 cagcaacgac gccggatttc tgactcgctg cgaggcctac ctcgacagtc aggggatgct
     1861 ccgatggtgg cggaagccga tccaagtctc cggggacgac ggctgtacct cgtccgcatg
     1921 acggactgcg gcactctggc cctggccgcg gcttttcgct cggcgggaat cgatgcggag
     1981 gtgcttccgc ccccggatgc ccgcacgctg ctgctggggg cacgccacct gaccggcgag
     2041 gagtgtctgc cggcgaaggt gacgctgggg gactatctga aggtagccga ggcgccggac
     2101 ttcgatccca ggcggacggc attcatgatg cccacgacgg aggggccgtg ccggttcggg
     2161 cagtatgctc ccttcatccg gaaggtcttc ggcgatctcg gttacccgga ggtggtgatc
     2221 ttctcgccca ccagcgggga cgggtaccag accaccgagg gtgtcggggc ctcgctcccc
     2281 cgattgtcct ggcgtgccct agtgacctca gacatcctgc ggaagctcct gctcaagacc
     2341 cgtccttacg agcggacccc gggagatgct gacgccgtct accacgattc actgcagacg
     2401 ctctgtgccg ccctggagcg gcgcggactc gggccccggg cacaccttca gctactgatc
     2461 gaaacgctca gcgggatccg cgaccggttt cggaccctgc cggctcgtta cgaacggggc
     2521 cggcttctga tcggggtcca gggggagatc ttctgtcgga tggaggagtt cagcaacgac
     2581 cagctcatcc gccacctgga agcctgtggc ggggaggctt ggctctcgga cctcggggag
     2641 tgggtatggt actgcaacaa cgaccaggag ctgtgcctgc gccgtgacgg gcggcgggtc
     2701 tctttcgcca tgctgggagc gaagctgcgg gatcggattc agcgcgccga cgaagcggct
     2761 ctccgggccc cctgccagga ggactttggc gggtacgaag atcccgagga tatcgagatc
     2821 atccttcgcg cggccatgcc ctacctccca cagtccgggg catcgggcga gatggtgctg
     2881 tcagcgggca aggtggacta tctcttccgg cggggcgtgg acgggatcat cgacatcagt
     2941 cccttctcct gcatgaacgg tatcgtctcc gaggccctct atccgcgcca gagccaggag
     3001 catgcgggac tccccataag gaacttctac ttcgacggta agggcaggga cctcaccagc
     3061 gacctggaga ttttcctgga attggcccga gcctaccaac agcagaagcc ttatcctcgc
     3121 cgctaccccc cctgcttcac gtgatcggtg cctgagtctc aatgcggggc ctgggatatc
     3181 gcgactctgc ttgaatgatg tgatgcaact gccggtacca acggcctcgc cgtcggttcg
     3241 caaagaccgc aaacccactt cccgacttga aagcgctggt tgggaatcac ccggagaaac
     3301 ggcccgaaag atttcgatcc tgaggatgtt ctggattgat gaggctggcg agttcttgcg
     3361 accttggcgg tgacagtatt gggctcagag tactcagacc ccgagacaaa tcgcaccaca
     3421 aagtcaccaa ggtaccaagg cagacgccaa cattctgatt ctcggccctg aaatgttgtc
     3481 agggcctgcc cggcccttcg gcatttcact tccaaatcct tcctggtcaa tc
//
LOCUS       DBNGZC010000932         1250 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2426924, whole genome shotgun sequence.
ACCESSION   DBNGZC010000932 DBNGZC010000000
VERSION     DBNGZC010000932.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1250)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1250)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1250
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2426924"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="AC16G0_16105"
     CDS             <1..449
                     /locus_tag="AC16G0_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017873382.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HIR5101178.1"
                     /translation="IAARAMQAGIALLKPGLAAQGGKPRGKVVIGTVKGDLHDIGKNL
                     VGMMLQGGGFEVIDLGADVPPQRFVEAVQANTPNFVMMSALLTTTMLSMKDTVEALKA
                     AGLRSRVKVGVGGAPVTQRFCDEVGADFYAPSATGAVTKAKEFLAV"
     gene            complement(942..>1250)
                     /locus_tag="AC16G0_16110"
     CDS             complement(942..>1250)
                     /locus_tag="AC16G0_16110"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5101179.1"
                     /translation="FGSLNVFNPRKAYDHVADFSIYVERAWRGKGVGSRLLRALIQRA
                     RHIGYHKLVLSAFPFNESGLALYKKFGFRVVGIYQEQGLLDGRWVDTIIMEKLLEGSA
                     "
BASE COUNT          230 a          388 c          406 g          226 t
ORIGIN      
        1 tcatcgccgc ccgggccatg caggccggga tcgccctcct gaagccgggt ctggccgccc
       61 agggcggcaa gccccgcggc aaggtggtca ttgggacggt gaagggggac ctccacgaca
      121 tcgggaagaa cctcgtgggg atgatgcttc aaggcggtgg cttcgaggtg atcgatcttg
      181 gcgccgacgt gcccccccag cgcttcgtgg aggcggtcca ggccaacacc cccaacttcg
      241 tgatgatgag cgccttgctc acgaccacca tgctctccat gaaggacacg gtggaagccc
      301 tcaaggcggc ggggctccgg agtcgggtca aggtcggggt gggcggcgcc cccgtgaccc
      361 agcgtttttg cgacgaggtc ggtgccgact tctacgcgcc gagcgcaacc ggtgccgtga
      421 caaaggccaa agagtttctc gcggtctagt cgatcagcac gcggcaacag gcggcgcaga
      481 gtacatccgc cgcctgttgt ccttcgtgtg caaggggggt atggaggcga aatgctccgg
      541 cgtcccagtg ggccctttct caggatcccc tgaaacccca aggcgttttc tttgcgtgct
      601 tggcgcacca acggcacggc cgtcggtgcc ggcggttgaa ctgcatcatt ccggccgaga
      661 gatcagggcg accaagatat cagcgcatga atgggcattg ggagagcggt gacgcgcttc
      721 gtgggctgac gatcacgtcg gttaatggat tggccgtctt ccaaaacgcg cattagaaac
      781 cacgtccccg aagttccagc aggttgcatc gcctcttgat cgcgtggatc gactgggaca
      841 acacggcggt tgccgtctta cgttcccctc tcgcagctcg acagttcact tcacaggcag
      901 gcgggactcg ggggcgtcac gcggcccata gcccgtcagg ttcaggccga gccctccagg
      961 agcttctcca tgatgatcgt atccacccag cgtccatcca gaaggccctg ctcctggtag
     1021 attccgacaa cccggaagcc gaacttcttg tacagggcca gaccgctctc gttgaaggga
     1081 aaggccgaga ggacgagctt gtgataaccg atgtggcggg cccgctgaat gagggcccgc
     1141 agcaggcggc ttcccactcc ctttccccgc caggcccgct cgacgtagat ggagaagtcc
     1201 gcgacgtggt cgtaagcctt gcggggattg aagacgttca ggcttccgaa
//
LOCUS       DBNGZC010000933         2280 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_470566, whole genome shotgun sequence.
ACCESSION   DBNGZC010000933 DBNGZC010000000
VERSION     DBNGZC010000933.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_470566"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1341
                     /locus_tag="AC16G0_16115"
     CDS             151..1341
                     /locus_tag="AC16G0_16115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HIR5101180.1"
                     /translation="MVSQESAEHIKQRVQAEVDKLTPDLLAISRFLHANPETAYEEHQ
                     AAELLSEVLQKHGFAVARDVAELPTAFTGRAGSGAPRITFLAEYDALPGLGHACGHNL
                     IGTASLGAALAVKTILDRVQGEVLLVGCPAEEKGGGKISLVEAGVFAGTDAAMLVHPS
                     NRTEMVKLALGMRELTVEFFGKASHAAAAPERGVNALDAVVVGYTAVGAMRQQVRPDA
                     RIHGIITHGGQAPNIIPDYAAARFFVRALDLAYMDELFRRVVNCFEAAAQATGCRVKF
                     SAASKDYHPYKPVYSFAEMFQRNLEALGESVDQGPVDRDLGSTDVGNVSQVVPTIQPM
                     LQISPREVTCHMPAFAEAAVSETGHQAMLRATKAMAMTAVDFLTDPKALARVAADFRG
                     EPSR"
     gene            1382..>2280
                     /locus_tag="AC16G0_16120"
     CDS             1382..>2280
                     /locus_tag="AC16G0_16120"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF015647.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HIR5101181.1"
                     /translation="MKVIGLMSGTSADGVDAALVEIGAEGDRPAVRLIAHETVPYPSG
                     LRERILTLASGGSSEEVCHLNAYLGELFAEAAQHVARRAGVSLADVDLIGSHGQTIQH
                     LPLPRPEGAHVVRSTLQIGEPAVIAERTGATTVADFRPRDLAAGGEGAPLTPLVHHLL
                     FSHPDRGRVILNLGGIANVTVLPAGGHPSTVTGFDTGPGNVLLDEFVRAAALSAKGYD
                     ENGRLAAAGRVHPTLLEELLSHPFIRRPPPKSTGRETFGPSLIREYRARLEHAGTNNL
                     DGLATLAAFTVAAVVQNLREFVFP"
BASE COUNT          361 a          763 c          792 g          364 t
ORIGIN      
        1 ccttgtccga gcactcggcc gcatggtatc ttccagcctc gtccgttcag gaagtctctc
       61 ggggatctga acgcggaagg gctcaccgat tttccgatgg cggagaaacg gcaggacctc
      121 ccttctagga gagcaccaag gagacgcagc gtggtcagcc aggagagtgc ggagcatatc
      181 aagcagcggg tccaggctga ggtggacaaa ctcacgccgg atctcttggc gatcagccga
      241 ttcctgcacg cgaatccgga gaccgcttac gaggagcacc aggcggccga gctcctgtcg
      301 gaggtgctgc agaagcacgg atttgcggtg gcgcgggacg tggccgagct gcccacggcg
      361 ttcacggggc gcgcgggatc cggcgcgccg cggatcacct tcctggccga atacgatgcc
      421 ctgcccggct tgggccacgc ctgcggtcac aatctgatcg ggacggcgtc gctcggagcg
      481 gccctggcgg tgaagacgat tctggaccgc gtccagggcg aggtgctcct ggtcggctgt
      541 ccggcggagg agaagggcgg cggtaagatc tccctggtgg aggccggggt cttcgccggg
      601 acggatgccg ccatgctggt gcatcccagt aaccggacgg agatggtgaa gctggcgttg
      661 gggatgcgcg agctgacggt ggagttcttc ggcaaggcga gccacgcggc ggctgccccc
      721 gagcggggag tcaacgcctt ggacgccgtg gtcgtgggct acaccgcggt cggggccatg
      781 cgacagcagg tacgccccga cgcgcggatc cacgggatca tcacccacgg cggccaggct
      841 ccgaacatca tccccgatta cgccgccgcc cgcttcttcg tgcgggccct cgacctcgcc
      901 tacatggacg agctgttccg gcgcgtggtc aattgcttcg aggcggcggc ccaggcgacc
      961 ggctgccggg tgaagttcag cgccgccagc aaggactacc acccctacaa gcccgtctat
     1021 tcgttcgccg agatgttcca gcgcaacctg gaggcgctcg gcgagagcgt ggaccagggg
     1081 ccggtggacc gggacctggg ctccaccgat gtgggcaacg tgagccaggt ggttcccacc
     1141 atccagccga tgctgcagat ttctccccgc gaagtcacct gccacatgcc tgccttcgcc
     1201 gaggcggccg tctccgagac gggccaccag gcgatgctcc gcgcgaccaa ggccatggcc
     1261 atgaccgcag tggatttcct gaccgacccg aaggcgctgg cgcgagtggc agccgacttc
     1321 cgcggcgagc cctcgcgctg agcggtcccc ggacgcccat gcattcctct ccagggctcg
     1381 gatgaaggtc atcggtctga tgtccggcac ctccgccgac ggcgtggatg ccgccttggt
     1441 ggagatcggg gcggagggag atcgcccggc cgtccgcctc atcgcccacg agaccgttcc
     1501 ctacccctcc ggcctgcggg aacgcatcct gaccctggcg tcgggtggca gcagcgaaga
     1561 ggtgtgccac ctgaatgcct atctcggcga gctgttcgcg gaggcggccc agcacgtggc
     1621 acggcgggcc ggtgtgtcgc tggcggacgt cgacctcatc ggctcccacg gacagacgat
     1681 tcagcatctt cccttgccgc gaccggaggg agcccacgtc gtgcggtcca cactccagat
     1741 cggagagccg gcggtgatcg ccgagcggac cggtgccacc accgtggccg acttccggcc
     1801 acgagacctg gcggccgggg gcgagggggc gccactcacc cccctcgtgc accatctgtt
     1861 gttcagccat ccggatcgcg ggcgggtgat cctcaacctg ggcgggatcg cgaatgtcac
     1921 tgttcttccc gcggggggcc atccctcgac ggtcacgggc ttcgacacgg ggccgggaaa
     1981 cgtcctgctg gatgaattcg tgcgggccgc ggccctgtcg gcgaagggct acgacgaaaa
     2041 cgggagactg gcggcggcgg ggcgggtaca tccgacgctc ctggaggagc tcctcagcca
     2101 tcccttcatc cgtcgccctc cgccgaagtc cacggggcgc gagaccttcg gcccgtccct
     2161 catccgtgag tatcgggcgc gtctggagca cgcggggacg aacaatctgg acggtctggc
     2221 aaccttggcc gcgttcaccg tcgcggccgt ggtccaaaat ctgcgagagt ttgtcttccc
//
LOCUS       DBNGZC010000934         2345 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_961710, whole genome shotgun sequence.
ACCESSION   DBNGZC010000934 DBNGZC010000000
VERSION     DBNGZC010000934.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_961710"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /gene="rplU"
                     /locus_tag="AC16G0_16125"
     CDS             <1..229
                     /gene="rplU"
                     /locus_tag="AC16G0_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR5101182.1"
                     /translation="VGNQVELDRVLLVSDGSSVRVGTPTLSGARVVSEIVAQTRGEKI
                     DVFKFKKRKKYRRKTGHRQELTQVKIAEIVA"
     gene            265..519
                     /gene="rpmA"
                     /locus_tag="AC16G0_16130"
     CDS             265..519
                     /gene="rpmA"
                     /locus_tag="AC16G0_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007640051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR5101183.1"
                     /translation="MAHKKGVGSSRNGRDSVGRRLGMKAFSGQAVSGGSIIVRQRGTR
                     FKPGKNVGIGGDDTLFAKIDGTVVIEHRGGNRFVSIAPAA"
     gene            606..1640
                     /gene="obgE"
                     /locus_tag="AC16G0_16135"
     CDS             606..1640
                     /gene="obgE"
                     /locus_tag="AC16G0_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012814555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR5101184.1"
                     /translation="MFIDTARILVVAGAGGRGCVSFRREKYVPRGGPNGGDGGHGGSI
                     YFEGSRSYQTLIDQKYQQLYRAGRGEHGRGKDQHGASAEDLVIKVPLGTVVRDLDTGQ
                     VLADLTQTGQRALVARGGRGGKGNARFATSIRQAPRFAEPGTPGETRTLLLELKLIAD
                     VGLVGLPNAGKSSLLAAFSAARPKIAAYPFTTLSPNLGVVDVPPYGGFVVADIPGLIH
                     GAAQGAGLGIRFLRHIERTRLLVQVVDVSETAEDPVAALTTVEAELLAYDPRLLRLPR
                     IVAANKIDQPHEGGLARLTAAATSRGLVLIPVSALTTEGVPTLVQEMAAALRASQQTS
                     SDLAQTVAGG"
     gene            1609..>2345
                     /gene="proB"
                     /locus_tag="AC16G0_16140"
     CDS             1609..>2345
                     /gene="proB"
                     /locus_tag="AC16G0_16140"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR5101185.1"
                     /translation="MTSRKPSPAVDRRKWLGRPKRVVVKVGSGVLSRGGIGLHRTVLA
                     DLAAALAGLLGQGLETILVSSGAILAGMEALGLPERPRDLPLKQAAAAVGQSHLMRAY
                     EEAFQPHGRRVAQILLTREDLRHRGRYLNARNTLFTLLRLGAIPIINENDTVAVQEIQ
                     FGDNDTLSALVANMAEADLLLILTDIEGLFSADPHRDTSARLIPVVRPQDAVAAFCAE
                     DTGSAASIGGMSSKVLAARRATAAGIPA"
BASE COUNT          390 a          773 c          813 g          369 t
ORIGIN      
        1 ggtcgggaac caggtggagc tggaccgggt gctcctggta tccgatggca gctcggtacg
       61 ggtcggtacg ccgacgctct ccggggcccg ggtggtttcc gagatcgtgg cgcagacccg
      121 gggggaaaag atcgacgtct tcaagttcaa gaagcggaag aagtaccgcc gcaagacggg
      181 ccaccgccag gagctgaccc aggtcaagat cgccgagatc gtcgcgtagc gatcgccggt
      241 ccggcggcgc gcacggaggc aggcatggca cacaagaagg gcgtgggaag ctccaggaac
      301 ggccgggaca gtgtcggccg gcggttgggg atgaaggcct tctccggcca agcggtcagc
      361 ggggggagca tcattgtccg gcagcgcggg acacggttca agcccggaaa gaacgtggga
      421 atcggcgggg acgataccct gttcgcgaag atcgatggga ccgtcgtcat cgagcaccgg
      481 ggtggcaatc ggtttgtttc gattgcgccc gccgcctgac cagattgtcg attgcagcag
      541 ttcgtcggat gtggatctga tccgagaggc tccgccaatc gtcaatcgcc aatcgtcaat
      601 cgccaatgtt cattgacact gcccgcatcc tggtggtcgc cggagcgggc ggtcgcggct
      661 gcgtgagctt tcgccgcgag aagtacgtgc cccggggcgg gccgaacggg ggggatgggg
      721 gccacggagg cagtatatac ttcgaaggca gtcggtccta ccagaccctc atcgaccaaa
      781 aataccaaca actctaccgt gccggacgcg gggagcacgg acggggcaag gatcaacacg
      841 gggcctccgc cgaggacctg gtcatcaagg tcccgttagg gacggtcgtc cgggatctcg
      901 acacaggcca ggtcttggct gatctcacac agacagggca gcgggccctg gtagctcgcg
      961 gcggccgcgg cggcaagggg aatgcgcggt tcgccacctc gatccgacag gccccccgtt
     1021 tcgccgagcc cgggacgccc ggcgagacgc gcacactcct cctcgaactc aagctcatcg
     1081 cggacgtcgg cctcgtgggt ctgccaaacg cggggaagtc ctcgctcctg gcggcttttt
     1141 ccgctgcgcg gccgaagatc gcggcctacc ccttcacgac cctcagccca aacctgggcg
     1201 tggtggacgt tccaccctac ggcgggtttg tggtggccga tattcccggc ctcattcacg
     1261 gcgcggcaca gggggcgggg ctgggcatcc gtttcttgcg ccacatcgag cggacgcgcc
     1321 tcttggtcca ggtggtggat gtgtcggaga cggctgaaga cccggtggcc gccctgacga
     1381 cggtggaagc ggagttgctc gcctacgacc cccgcctgct gcgtcttccg cggatcgtcg
     1441 ccgccaacaa gatcgaccag ccccacgagg gcgggctcgc gcgcctcacc gcggcggcca
     1501 cctcgcgcgg attagtcctg atccctgtct cagccctgac gaccgaagga gtccccaccc
     1561 tcgtgcagga gatggctgcc gcccttcggg cgtcgcagca gacgtcgagt gacctcgcgc
     1621 aaaccgtcgc cggcggttga ccggcgaaaa tggcttggcc gcccgaagcg ggtggtggtg
     1681 aaggtgggct ccggtgtcct gtcccgcggg ggcatcggcc tccaccggac agtcctggcc
     1741 gatctcgccg cagccctggc cgggctgctg ggacagggtc tggagacgat cctggtgtcc
     1801 tcaggggcca tcctcgccgg catggaggcc ttggggctcc ccgagcggcc ccgcgatctc
     1861 ccgctgaagc aggccgccgc cgccgtcggc cagagccacc tcatgcgggc ctacgaggag
     1921 gcgttccagc cccacggtcg gcgcgtagcg caaattctcc tgacccggga agaccttcgc
     1981 catcggggac ggtacctcaa cgcgaggaac accctcttca cgcttctgcg tctgggggcg
     2041 attcccatca tcaacgagaa cgataccgtg gccgtgcagg agattcagtt cggggataat
     2101 gacaccctct cggcgctggt ggcgaacatg gccgaggcgg acctcctgct gattctgacc
     2161 gacatcgagg ggctgttcag cgcggatccc caccgggaca cctccgcccg cctgattccc
     2221 gtggtgcgac cgcaggacgc cgtggccgcc ttctgcgccg aggacaccgg cagcgcggcc
     2281 agcatcggtg gcatgagttc gaaggtcctg gcggcccggc gcgccaccgc ggcgggcatc
     2341 cccgc
//
LOCUS       DBNGZC010000935         3741 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_470778, whole genome shotgun sequence.
ACCESSION   DBNGZC010000935 DBNGZC010000000
VERSION     DBNGZC010000935.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_470778"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="AC16G0_16145"
     CDS             complement(<1..1006)
                     /locus_tag="AC16G0_16145"
                     /inference="COORDINATES: protein motif:HMM:NF025875.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR5101186.1"
                     /translation="MTWAATAAEKPAGLSDAACLNCHADPALARLQPGQGGRSMYVDR
                     TVLARSQHAKVACSSCHAGITKFPHDSGSGKATCATCHQREAKAYAGSIHGRGLAKAD
                     ADVPTCQRCHGGHDILAVGDPASRVQPLHQAKICMGCHADTRITARHAEMPNVLLIRA
                     YADSVHGRAVARSGLTVAPTCTSCHGTHALMPADDPGSPVHRGNVAATCGRCHQGILA
                     EYRDSIHAAALDRGVKEAPTCTNCHGEHTIAAPTDPTSAVAPRNIAKTCGACHAAEGI
                     VAKIGLAAKRGETYADSFHGIALKFGNSEAANCASCHGFHDIRPSSDPRSRVAKANLP
                     A"
     gene            complement(1158..3266)
                     /locus_tag="AC16G0_16150"
     CDS             complement(1158..3266)
                     /locus_tag="AC16G0_16150"
                     /inference="COORDINATES: protein motif:HMM:NF013460.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HIR5101187.1"
                     /translation="MTTFFIGLVLGLLAVANDTSAAGPTSQDCLGCHADKSLTKEVAG
                     KSVSLFADEAVLKASAHGRLECTACHTGISEIPHPEKLAPVSCQKCHARAAGTFTQSV
                     HGRKGGPGLPCQDCHGTHGIAPGKQLGTTPCRACHDPTIQAYIGSVHGRALANGVKEA
                     ALCFNCHGEAHQLRNQADPASPTNRARMAETCGRCHADRALVERRHIPIPQAYQLYQK
                     SVHGRAVAAGKPAATCDDCHESHDLRRANDPKSSVYRENIPKTCGKCHAEESKAYLAS
                     IHGTAMRQGVMKSPVCTDCHGEHSIRAAQDPDSRVSVAHVSQTCGNCHEAEGISEKYG
                     IPGGRLESYADSFHGLAVRGGSKVAANCASCHGVHDIRPSSDPKSAISTGNLPATCGK
                     CHPGAGENFAKGKVHVTLTAHEQPILFYVRNFYLLLILVTISGMGAHNALDFFKKLRR
                     EYRQQSGGEAALEAGEFNGQPRVVQGWFLRMALTERWQHGLLATSFMVLVYTGFALKF
                     PDTWLFAWFVALEKGYAWRGWVHRGAAVLMVMACLWHLAYLMTRRGRSHLLAMLPRFE
                     DVKETLQNIGHLLGLRAEPPRFDRFSYIEKAEYWALIWGSVVMVVTGGLLWFENLSLR
                     FLAKWMLDVATMVHYYEAWLATLAIIVWHFYYVIFNPDVYPMNWTWLTGRISEETLRH
                     EHPREYERLREHGGLGPPTA"
     gene            complement(3664..>3741)
                     /locus_tag="AC16G0_16155"
     CDS             complement(3664..>3741)
                     /locus_tag="AC16G0_16155"
                     /inference="COORDINATES: protein motif:HMM:NF025875.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR5101188.1"
                     /translation="GACHDGKVAFSVKDAASCTKCHQKG"
BASE COUNT          650 a         1202 c         1206 g          683 t
ORIGIN      
        1 tggcgggaag attcgccttg gcgacgcgcg agcgggggtc gctggacggc cggatgtcgt
       61 ggaatccgtg acaactcgca cagttcgccg cttccgagtt cccgaacttc agggcgatcc
      121 cgtggaagct gtccgcgtag gtctccccgc gcttggccgc caagccaatc ttggccacaa
      181 tcccttcggc cgcgtgacag gcgccgcagg tcttggcaat gttccgcggc gccaccgcgg
      241 atgtggggtc cgtgggcgcg gcgatggtgt gctcgccgtg gcagttggtg caggtgggcg
      301 cctccttaac ccctcgatcc agggcggcgg cgtggatgct gtcccggtat tcggccagga
      361 tcccctgatg gcatcgcccg caggtcgcag ccacgttccc gcggtggacg ggagagccgg
      421 gatcatctgc cggcatcagg gcgtgggtgc cgtgacaact ggtgcaggtg ggcgccaccg
      481 tcagtccgct cctggccacg gccctgccgt ggacgctgtc cgcataagcc cggatgagga
      541 gcacgttcgg catctccgca tgccgcgcgg tgatccgggt gtcggcatga cagcccatgc
      601 agatcttggc ctggtggagg ggctgtaccc gggacgccgg atcccccacg gcgaggatgt
      661 cgtgtccgcc gtggcagcgc tggcaggtcg gtacgtcggc atccgccttc gccaggccgc
      721 gcccgtggat gctgccggcg tacgcctttg cctctcgctg gtggcaggtg gcgcacgtag
      781 ccttgccgga cccggaatcg tggggaaact tggtgatccc cgcgtgacag gatgagcagg
      841 cgaccttggc gtgttgcgac cgggccagga cggtccgatc aacatacatg ctccggccac
      901 cctggccggg ctgcaacctg gccagagccg gatccgcgtg gcagttgaga cacgccgcgt
      961 cggacaggcc tgcgggtttc tccgccgcgg tcgccgccca ggtcacgcct gacaggagga
     1021 gaatggcgac gcagctgcac caggctgtct tgcggactgc gtctgacgca tggcgcatgg
     1081 cccgaaacgg atctcgggcg cagatcaccc tctgccacac gccataggcc attggccccc
     1141 ccaccccact cctcgcctca agccgtcgga ggtccgagtc ccccgtgctc acgcagccgc
     1201 tcgtactcac ggggatgctc gtggcgcagc gtctcctcgg agatcctccc cgtcaaccac
     1261 gtccaattca tggggtagac gtcgggattg aagatcacgt agtagaagtg ccacacgatg
     1321 atcgccagcg tggccagcca ggcctcgtag tagtgaacca tggtggccac gtccagcatc
     1381 cacttcgcca ggaacctgag cgagaggttt tcaaaccaga gcagacctcc cgtgaccacc
     1441 atcaccacgg aaccccagat gagcgcccag tactctgcct tctcgatgta gctgaagcgg
     1501 tcgaagcgcg ggggctccgc ccgcagcccg aggagatgcc cgatgttctg gagcgtctct
     1561 ttcacatcct cgaagcgcgg cagcatggcc aggaggtgcg atctgccccg ccgggtcatc
     1621 agatacgcca ggtgccacag gcacgccatg accatgagaa cggccgcgcc gcgatggacc
     1681 cacccgcgcc acgcgtatcc cttctcgagc gccacgaacc aggcgaaaag ccacgtgtcg
     1741 ggaaacttca aggcgaagcc ggtgtagacc aagaccatga agctcgtggc gagcaacccg
     1801 tgctgccagc gctctgtcag cgccatgcgc aggaaccagc cttggacgac gcgaggctga
     1861 ccgttgaatt cgccggcctc cagggcggcc tccccgccgc tctgctggcg gtactcgcgc
     1921 cggagcttct tgaagaagtc gagggcgttg tgggccccca tcccggagat cgtgaccagg
     1981 atcaggagga ggtagaaatt tcgaacgtag aacaggatcg gctgttcgtg ggcggtgagg
     2041 gtgacgtgga ccttgccctt ggcgaaattt tcgcccgccc caggatggca tttcccgcag
     2101 gtggcgggca ggtttcccgt ggagatggcc gacttgggat cgctggacgg ccggatgtca
     2161 tgaacgccgt gacaactggc gcagttcgcc gcgaccttgg agccgccccg aaccgccagc
     2221 ccgtggaagc tatcggcata actttccagg cggcctccag gaatcccgta cttctcgctg
     2281 atcccctcgg cctcgtggca gttcccgcag gtttgggaca catgcgccac gctcacccga
     2341 gagtcaggat cctgggccgc acggatcgaa tgctccccgt gacaatcagt gcagacaggc
     2401 gacttcatca ccccctgcct catggcggtc ccgtggatgc tggccagata ggccttgctc
     2461 tcttcagcat gacacttgcc gcacgtcttg gggatgttct ctcggtagac ggaggacttg
     2521 ggatcgttgg ctcgccgcag atcgtgactc tcatggcagt catcgcaggt ggccgccggt
     2581 ttccctgccg ccaccgctcg accgtggacg ctcttctggt agagctggta cgcctgggga
     2641 atcggaatgt gccggcgctc gaccagggct cggtcggcgt ggcagcgtcc gcaggtttcc
     2701 gccatgcggg cccgattggt gggcgaggcc gggtccgcct gattccgaag ttggtgcgcc
     2761 tcaccgtgac agttgaaaca cagcgccgcc tccttgactc cgttggccaa ggcccggccg
     2821 tgcacgctcc caatgtaggc ctggatggtc ggatcgtggc aggcccgaca gggagttgtc
     2881 cccagttgct tgcctggggc gatcccatgg gtcccatggc agtcctgaca ggggagcccg
     2941 gggcccccct tgcggccatg gacgctctgg gtgaacgttc ccgcggctcg ggcgtggcac
     3001 ttctggcagg agacgggcgc cagcttctcg gggtgaggaa tctcgctgat gccggtatgg
     3061 cacgccgtgc actccagacg gccatgcgcc gaagccttca ggactgcctc gtccgcaaaa
     3121 agggagaccg acttgcccgc gacttccttc gtcaggctct tatctgcgtg gcagccgagg
     3181 caatcctggc tcgtcggtcc cgctgccgaa gtatcgttgg ccacggcgag gagacccaag
     3241 accagcccga taaagaaagt tgtcatcagt atgcgtttca ttccgcacca ttcccgtata
     3301 gaggggtaaa agcctaaagg gattgagcaa cttatgtgcc ggtaagtgca tgtcaacagg
     3361 agtaaatcac accctgttct cctgttcttg tcctgttgaa tttcacaaca cttacacccg
     3421 actagatggg acggattcaa ccgggcatac caagctcccg aaagaggcct cacagggttg
     3481 tctcatcttg ttgccggagt gtgccatttc ccacggaatt agggtcatcc gggaaacggc
     3541 tgggaacagg aacggatcag ctacctgggc gggaatcgca cagagcaaga atgcgaaagc
     3601 ccacccaggc tcttccccgg atgggcttcc acattcaggc gaggcgagaa gtcgaggatg
     3661 ttatcagccc ttctgatggc atttggtgca gctggctgca tccttcacgc tgaaggcgac
     3721 cttgccgtca tggcaggccc c
//
LOCUS       DBNGZC010000936         5454 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_81665, whole genome shotgun sequence.
ACCESSION   DBNGZC010000936 DBNGZC010000000
VERSION     DBNGZC010000936.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5454)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5454)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5454
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_81665"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /gene="murD"
                     /locus_tag="AC16G0_16160"
     CDS             complement(<1..1114)
                     /gene="murD"
                     /locus_tag="AC16G0_16160"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR5101189.1"
                     /translation="MSHRPACQTEFRGKRVTVVGLARSGFAASKILAARGASVLATDR
                     SPAEKLRTNLAELEECGVRVETGGHSPPGFLETDLLVISPGVDGRLPLIQQARATGIP
                     VWGEVELAYRLTAAPFIGITGTKGKGTTATLLGEILRQGGKDAVVAGNIGRPLCEVVP
                     GLSPETWVVAELSSFQLEEIVTFRPRIAVLLNLAPDHLDRYPDVQTYYAAKQRLFENQ
                     GREDSVVLNADSASVLALARGSQARRFQFSRRGPVGSGAFLRDDCFFLETGGVPQKVC
                     PRRELGVPGPGIVEDALAALAVANVLGVAAEPMAEALRGFRGRPHCLELVGEVGGVRY
                     VNDSKATNVFAVRRALEAMEHPVILIAGGRDKREDFR"
     gene            complement(1254..2336)
                     /gene="mraY"
                     /locus_tag="AC16G0_16165"
     CDS             complement(1254..2336)
                     /gene="mraY"
                     /locus_tag="AC16G0_16165"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR5101190.1"
                     /translation="MLYHLLVPLATYFSPLNVFRYVTFRTAGAFLTALLVALLLGPVV
                     IRKLTDMRARQVIRDDGPQIHLKKAGTPTMGGVLILLAITSATLLWAQLNNRFIWLTL
                     LATLAMGAVGFVDDFLKLKRANSVGLRPRQKFFWQILIGLLVGTYLYLFPADDYATKL
                     AIPFFKRWLPELGIWYILFAMVVLVGCSNAVNLTDGLDGLAIVPTLMAAATFTLFAYV
                     AGHATLSRYLQVIFVKGASELPIFAAAMVGASLGFLWFNAHPAQVFMGDTGSLALGGA
                     LATLALITKNELVLVMAGGCFVVEALSVIIQVFWFKRTGRRVFRMAPIHHHYELNGLP
                     EPKIIVRFWIVSFVLQVIALTTLKLR"
     gene            complement(2437..3696)
                     /locus_tag="AC16G0_16170"
     CDS             complement(2437..3696)
                     /locus_tag="AC16G0_16170"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR5101191.1"
                     /translation="MKRVGLIFGSRSVEHEVSVTTASKAYEALRQLAGEYETVLLYVN
                     RAGAWLSGKAVTRLLEIEAEGRASQDGRRRKNLQQEFKRALESLERGTGSKDAEPLYI
                     SPDPGLRHLCQDPERGGWFRRHVAPGLDIAFPTVHGTHGEDGTLQGLFELADLAYVGP
                     GVAASAAGMDKILSKLLFRGAGLPTLPATWFSRKQWLMGEAAVIAKVEASLIYPVVVK
                     PAVAGSSVGIGRAGSAAELKRAVGQAIQFGTRVLVERALDDRLEVQCAVLGNHELEVS
                     LCEELVRGAGIVSFEDKYLRRAERQEEADLAPSHIPARIPEELARRVRELALGAFRAL
                     DARGISRVDCLVERSSMQPFVNEVNTLPGSLCLRLWEASGVSSTTLIRRLLELAVEAH
                     EEKVATRFESQEGKDLVDKKHLMAPGK"
     gene            complement(3693..5330)
                     /locus_tag="AC16G0_16175"
     CDS             complement(3693..5330)
                     /locus_tag="AC16G0_16175"
                     /inference="COORDINATES: protein motif:HMM:NF019850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HIR5101192.1"
                     /translation="MALVRAILPYALVVVRRHLDCLYILQLERYDPSRYARWLGRHRR
                     LVLGAGEIATQVSLTLAATLLTWAGAHWLVSCGLWVAAGHLALVRQKSLQVSQRFQTT
                     PRAVRLACVVGGISAGLLLGMVAVGGAIRFAPGLSARLVVGGIVGMALAGLMAPVSVW
                     CAGRLLAPVERWIAQRFLQEADRRMRAYPGRVIGITGSYGKTSTKLILADLLASRYRV
                     LKTPAGVNTTMGITRVIREELSDTHEYFVVECSAFGPGEIRAVCDIVHPSLGILTAVG
                     IQHLERFGTPDRIAEAKYELLAALPAGAPAVVNADDPMCLRLAQRARAEGKRVLLYGM
                     GETEAGLAIRGTDMTLRSRGPSFRVVAGDGQTICFETRLLGRWNLSNILAGLAAAVEC
                     GVPLSAMRDAVAALAPAPRRLEVKEEAGIVKILDVANSNPRGAEMALEVLAQFPGGSR
                     ILITPGMVELGSSEAEENRRFGEKAAATCDYVVLVGAEQTRPIREGLAQRGFPADRVL
                     MARNADEVADRLAGIVRQGDVLLYENRLPDTYLEVGP"
BASE COUNT          911 a         1972 c         1751 g          820 t
ORIGIN      
        1 gacgaaaatc ttctcgcttg tcccgcccac ccgcgatgag gatgacgggg tgctccatgg
       61 cctccagggc gcggcggacg gcgaagacat tcgtggcctt cgagtcgttg acatagcgga
      121 cgccgccaac ctcacccacg agttccaggc aatgcggacg accgcgaaac ccgcggagcg
      181 cttccgccat gggttcggca gccacgccca gcacgttggc cacggccagc gccgccaggg
      241 catcctccac gatgccgggg ccgggtacgc ccagctcccg ccgcgggcag accttctggg
      301 ggactccccc ggtctccaag aagaagcagt cgtcccggag aaacgccccg cttccgacag
      361 gaccgcgccg gctgaactgg aagcgccgag cttgactccc cctggctaac gccaggaccg
      421 acgcagaatc cgcgttgagc accacgctgt cctcgcgacc ctggttttca aacagacgct
      481 gcttggccgc gtagtatgtt tggacatccg ggtatcggtc gagatgatcc ggggccaggt
      541 tcagcaacac ggcaatccgg ggtcggaacg tcacgatctc ctccagctga aaactggaga
      601 gttccgccac cacccaggtc tccggagaca ggccgggcac cacctcgcac agcggccggc
      661 cgatgttccc ggcaaccacg gcgtccttgc ccccttgccg caggatctcc cccaggagcg
      721 tcgcggtcgt ccccttgccc ttggtcccgg tgattccgat gaacggggcc gccgtcagcc
      781 gataggccag ctccacctcg ccccagaccg ggatgcccgt ggcccgcgcc tgctggatga
      841 ggggcaggcg cccgtccaca cccggactga tcaccagcag gtccgtctcc aggaagcccg
      901 gtgggctgtg accgccggtc tcgaccctga cgccgcactc ctccagctcc gccaggttcg
      961 tacggagctt ctccgccggg ctccggtcgg tggccaggac gctcgcaccg cgcgcggcca
     1021 ggatcttgct ggccgcgaag ccgctccggg ccagtcccac gaccgtgacg cgcttccccc
     1081 ggaactccgt ctggcaggct ggccggtgac tcatgggttc acgttcaagg ttcgaggttc
     1141 aaggtccaag gttcgaagtt cacgacgtgt cgccggccgt ccacacgttc catccggcct
     1201 ccccgctcgc tgcccatccc ggtcgctgtc cctgtccctg tcacgctcgg ccctcagcga
     1261 agcttgagcg tcgtgagggc gatcacctgg aggacgaagg acacgatcca gaagcggacg
     1321 atgatcttcg gctcgggcag gccgttcagt tcgtagtgat ggtggatggg cgccatccgg
     1381 aagacccgcc gtccggtccg cttgaaccag aaaacctgga tgatcaccga gagggcctcc
     1441 accacgaagc agccgccggc catcaccagg accagttcgt tcttggtgat gagggccaac
     1501 gtggccaggg cgccccccag ggccagagat cccgtatcgc ccatgaacac ctgggccgga
     1561 tgggcgttga accagaggaa ccccaggctc gcccccacca tggcggcggc gaagatgggc
     1621 agctcgctgg cgcccttcac gaagatcacc tggaggtagc gggagagggt cgcatgaccc
     1681 gccacgtaag cgaacagggt gaaggtcgcg gcggccatga gcgtggggac gatggccagg
     1741 ccgtccaggc cgtcggtcag gttcacggcg ttggagcatc ccaccaggac caccatggcg
     1801 aagaggatgt accagatgcc cagctccggc agccagcgtt tgaagaaggg gatggccagc
     1861 ttggtggcat aatcgtcggc ggggaagaga tacagatagg tccccaccag gaggccgatc
     1921 aggatctgcc agaagaactt ctgcctcggg cgcagtccga cactattggc ccgcttgagc
     1981 ttgaggaagt cgtccacgaa tcccacggcc cccatggcca gcgtggcgag gagggtgagc
     2041 cagatgaatc gattgttgag ctgcgcccag aggagcgtgg cggaggtgat cgccaagagg
     2101 atcaggactc cccccatggt gggggtgccc gccttcttca gatgaatttg cggaccgtcg
     2161 tctcggatca cctggcgggc ccgcatgtcc gtgagcttcc ggatgacgac ggggccgagg
     2221 agcagggcga ccaagagcgc agtgaggaag gcccccgccg tccggaacgt gacgtagcgg
     2281 aagacgttga gcgggctgaa gtacgtcgcc agcggaacca gcagatggta gagcacgtat
     2341 catccttctt atcggcagcc accagcttct cggccgtcag ccggggcacc ctgcctccga
     2401 tgacggctga cctctgatcg ctgcccgctg ccctgcctac ttccccgggg ccatcagatg
     2461 cttcttgtcc accaggtcct tcccctcctg cgattcgaag cgcgtggcga ccttttcctc
     2521 gtgcgcctcg accgccagct ccaggaggcg ccggatcagc gtggtcgacg agacaccgct
     2581 ggcctcccac aggcgcaggc agagcgatcc ggggagggta ttgacctcgt tcacgaacgg
     2641 ttgcatggag gaacgttcca ccaggcaatc cacgcgcgag attccgcggg cgtccagggc
     2701 ccggaaggcg ccgagggcga gctcccgcac ccgccgggcc agctcttccg ggatccgggc
     2761 cgggatgtgg ctgggcgcga gatcggcctc ctcctggcgc tccgctcgcc ggaggtactt
     2821 gtcctcgaag ctcacgatgc cggccccccg gacgagctcc tcacacaggg agacctccag
     2881 ctcgtggttg cccagaaccg cgcactggac ctccaggcgg tcgtccagcg cccgctccac
     2941 caggacgcgc gtgccgaact ggatcgcctg acccaccgcg cgcttgagtt ccgcggcgct
     3001 ccccgcccgg ccgatcccga cgctggagcc cgccaccgct ggcttgacca ccaccgggta
     3061 gatcaggctg gcctccacct tggcgatcac cgctgcctcg cccatgagcc actgcttccg
     3121 gctgaaccac gtcgccggga gcgtcggcag cccggcgccg cggaagagca gcttggagag
     3181 gatcttgtcc atgcccgcgg ccgaggccgc cacccccggg cccacgtagg cgaggtccgc
     3241 cagctcgaac agaccctgga gcgttccgtc ctcgccgtgg gtcccgtgga ccgtgggaaa
     3301 ggcgatgtcc agcccgggcg ccacgtggcg ccggaaccag ccgcccctct ccgggtcctg
     3361 gcagagatgc ctcagcccgg ggtccggcga gatatacagg ggttccgcat ccttggatcc
     3421 ggtcccgcgc tccaggctct ccagggcccg cttgaactcc tgctgcaggt tcttccggcg
     3481 ccgaccgtcc tggctggccc ggccctcggc ctcgatctcc aggagccgcg tgacggcctt
     3541 gcctgagagc caggctcccg cccgattcac atacaggagg accgtctcgt actcgcccgc
     3601 gagctggcgc aaggcctcgt aagccttgct ggcggtcgtc accgagacct cgtgctccac
     3661 cgagcggctg ccgaagatga ggccgacccg cttcatggtc caacctccag gtaggtgtcc
     3721 gggagccggt tctcgtacag caagacgtcc ccctgccgga cgatccccgc gagtctatcc
     3781 gccacctcgt cggcgttcct ggccatcagg acccgatccg cggggaagcc gcgctgggcg
     3841 aggccctcgc ggatggggcg ggtctgctct gcccccacca gcaccacgta atcgcaggtg
     3901 gccgccgcct tctccccgaa gcgccggttc tcctccgcct cgctggatcc gagttcgacc
     3961 atccccggtg tgatgaggat gcgtgagccc cctggaaact gggccagcac ctccagggcc
     4021 atctccgccc cccgagggtt gctgttggcc acgtccagga tcttgacgat gcctgcctct
     4081 tccttgacct ccagccgtcg cggggcgggg gcgagtgcgg ccacggcatc ccgcatggcg
     4141 gagagcggca cgccgcactc caccgccgcc gccaggccag ccaggatgtt ggagaggttc
     4201 cagcgcccca ggagacgcgt ctcgaagcag atcgtctggc cgtcgcccgc cacgacccgg
     4261 aaactgggtc cccggctccg cagcgtcata tccgtcccgc gaatggcgag ccctgcctcc
     4321 gtctcgccca tcccgtagag gagcacgcgc ttcccctccg cccgggcccg ctgcgccaac
     4381 ctgaggcaca tcggatcgtc cgcattcacc accgccggcg cccccgcggg cagggccgcc
     4441 aggagctcgt acttcgcctc ggcgatccga tccggcgtgc cgaaccgctc cagatgctga
     4501 atccccaccg ccgtcaggat ccccagcgac gggtgcacga tgtcgcagac cgcgcggatc
     4561 tcccccggcc cgaaggccga gcactccacc acgaaatact cgtgagtgtc cgagagctcc
     4621 tcgcggatga cccgggtgat ccccatggtg gtgttgacgc cggccggcgt cttgaggacc
     4681 cggtagcgcg acgccaagag atcggccagg atgagcttcg tgctcgtctt gccgtagctc
     4741 cccgtgatgc cgatcacccg gcccgggtaa gcccgcatgc gacggtcggc ctcctgcaag
     4801 aatcgctggg cgatccagcg ttccacgggc gcaaggagcc tgccggcgca ccacacggaa
     4861 accggcgcca tcagaccggc cagggccatg cctacgattc cgccgaccac gaggcgcgcg
     4921 gaaagacccg gcgcgaaccg gatggcgcct cccaccgcca ccatccccag caggagcccg
     4981 gcgctgattc ccccgaccac gcaagccagc cggacggctc gtggggtggt ctggaagcgt
     5041 tggctcacct gaagcgactt ctgccgcacc agggcaaggt gcccggcggc gacccacaag
     5101 ccgcaggaga cgagccagtg agcgccagcc caggttagga gcgtggcggc gagggtgagg
     5161 gaaacctgcg tcgcgatctc ccccgcgccc aggacgagtc gccggtgcct gcccagccag
     5221 cgcgcgtacc gagacgggtc gtatcgctcc agttggagga tgtagaggca atcgagatgc
     5281 ctccgcacca cgaccagcgc gtaggggagg atggcgcgaa caagcgccat gatgcctcgc
     5341 gtcaccggac cgctcaccct gacgtcccct cgtgcaagaa ggcggtcagc ctccggccga
     5401 actcctccgg cgcgtccagg aaggggaagt gccccgcttc ctcgatcacc acca
//
LOCUS       DBNGZC010000937         1950 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_178301, whole genome shotgun sequence.
ACCESSION   DBNGZC010000937 DBNGZC010000000
VERSION     DBNGZC010000937.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_178301"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            355..879
                     /locus_tag="AC16G0_16180"
     CDS             355..879
                     /locus_tag="AC16G0_16180"
                     /inference="COORDINATES: protein motif:HMM:NF017416.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR5101193.1"
                     /translation="MLTPTDHQRSFYDADHVCESLIPPDSFYRKFREVVAPLITDTQF
                     AAMYSLNTGRPAIPPSLLACATTLQFHRNLSDREMERACSYDIEVKYALGLRLDERPF
                     DHSSLGDFRQRLLTNGKEEAVFDQILHRLVATGLITRNEIQRIDATHVLADIAIPTTI
                     TLITMGIGAVLKPL"
     gene            1009..1950
                     /locus_tag="AC16G0_16185"
     CDS             1009..1950
                     /locus_tag="AC16G0_16185"
                     /inference="COORDINATES: protein motif:HMM:NF013752.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR5101194.1"
                     /translation="MVADARTVLARTQALTGDPLLARWVETLRRILRENFEDDEAGGP
                     REKRYQAKGSDLLVSPIDPDARYDAKSDTKRFTGYKANVTETVTGRFITNITALPGNR
                     PDGEPTVEAVVEQRGVGLLPATLIGDTAYSTGRARQALKGNGMELVGPLRDPAHPQDL
                     PQADVSLRPRPEHPDVTCGGDGTADCLRHPRPIRKFHFPLPVCRACPRRSDCTAAKDG
                     RWSFGISDPHEELCATEAYNLTTAFQTTMKLRPPIEGKLSELVRYHGLRRARYRTLVK
                     VRLQRYFTAAAVNINRWFALLSQPWTVGNGDPVPARG"
BASE COUNT          375 a          692 c          580 g          303 t
ORIGIN      
        1 cgccataaga tcgctaactg attttacatg gtctcgtccg cttttacgtg gtctcgtccg
       61 caggatcccc gaaccaactt gatacagccg gaaaagccct cgggacgaac ggatgccagt
      121 acgccggagg caaccccatc caatccatcg acctgcaggg ccgagtcgtg taatgccccg
      181 gaaccccgga agcctctggc agacggaccg gcgaagcctc cggccctggt gcgaggtggc
      241 tgcggatcaa gaatctgtgg gggatgcgtt ttcaggtgct cctgcctctt ggcattggct
      301 gtcgcctgcc tatttgtaat aagggaggca gcgcatgaca cgagagcccc acggatgctg
      361 acgcccacgg atcaccaacg cagcttctac gatgccgacc acgtctgtga atccctcatt
      421 ccccccgaca gcttctaccg caagttccgc gaggtcgtcg cccctctcat caccgataca
      481 cagttcgccg cgatgtactc actcaacacg ggccgtcccg cgattccgcc gtcgctcttg
      541 gcctgcgcca ccaccctcca gttccaccgg aacctctcgg accgggagat ggagcgagcc
      601 tgcagctacg acatcgaggt aaagtacgcc ctcgggctcc gcctcgatga gcggccgttc
      661 gaccactctt ccctcggcga tttccgtcag cggctcctga cgaacgggaa ggaggaggcg
      721 gttttcgacc agatcctcca tcggctcgta gccaccggcc tgatcaccag gaacgagatc
      781 cagcggatcg acgccacgca cgtcctggcc gacatcgcca tccccaccac gatcacgctc
      841 atcacgatgg gtatcggcgc agtcctgaag cccctctaga agcggcacaa gaccatcctc
      901 cagcaactcg gagagaccat cgctgtcgac gagtacacca aggccaagat caccccggcg
      961 ggcgccggcg ggcacgacct ggggcggaag aggcggaagc tcgtggcgat ggtagccgac
     1021 gcccggacgg tcctcgcgcg cacgcaagcc ctcacgggcg accccctcct cgcacgctgg
     1081 gtcgagacgc tacggcggat cctccgcgag aacttcgaag acgacgaggc cggggggccg
     1141 cgggagaagc gctaccaggc gaagggatcg gatctcctgg tctccccgat cgaccctgac
     1201 gcccgctacg acgcgaagtc ggataccaag cgcttcactg ggtacaaggc gaatgtcacg
     1261 gaaaccgtaa ccggccggtt catcaccaac atcacggcgc tccccgggaa ccggcccgat
     1321 ggcgaaccga ccgttgaggc ggtggtcgag cagcgcgggg tcggcctcct ccccgccacg
     1381 ctgattggcg ataccgcgta cagcaccggg cgcgctcggc aggccctcaa gggaaatggg
     1441 atggagctcg tcgggcccct ccgcgacccc gcgcacccgc aggatctacc ccaagcggat
     1501 gtttcactac gaccgcgccc agaacaccct gacgtgacct gcgggggtga cggtacagca
     1561 gactgtctcc gacacccccg cccgattcgg aagttccact tcccgctccc cgtgtgcagg
     1621 gcctgccccc gccggtctga ctgcacggcg gccaaggacg gccgctggag cttcggaatc
     1681 agcgatcccc acgaagagct ctgcgcgacc gaggcgtaca acctcaccac ggccttccag
     1741 acgaccatga aactccgccc gccgatcgaa gggaaactct ccgaactcgt tcgctatcac
     1801 ggcctccgcc gggcacgcta ccgcaccctg gtgaaggtcc ggctgcagcg ttacttcacg
     1861 gcggccgcgg tcaacatcaa ccggtggttc gcgctgctct cgcagccgtg gacggtgggg
     1921 aatggggacc cggtcccggc gaggggatga
//
LOCUS       DBNGZC010000938         3016 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2034509, whole genome shotgun sequence.
ACCESSION   DBNGZC010000938 DBNGZC010000000
VERSION     DBNGZC010000938.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2034509"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="AC16G0_16190"
     CDS             <1..288
                     /locus_tag="AC16G0_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013010796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein C-terminal
                     domain-containing protein"
                     /protein_id="HIR5101195.1"
                     /translation="LARALVSEPKLLLLDEPAAGLNAAETEVLVDLLFQICDRGITIL
                     LVEHDMSLVMNVSDHITVLNFGKKIAEGSAEVIEKNQEVIDAYLGTEVGNA"
     gene            281..1045
                     /locus_tag="AC16G0_16195"
     CDS             281..1045
                     /locus_tag="AC16G0_16195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019240244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5101196.1"
                     /translation="MLKVQGIETWYGAIQALKGVSLDVKQGEIVALLGAYGAGKSTTI
                     KTITGQLKPARGRIEFTGQDITGREVEDIAEAGIACVPEGRRIFAGLSVMDNLMLGAT
                     PRKNAPKADIQRDLDRIFTLFPILKEFKDRLGWQLSGGQQQMLAIGSGLMSGARLLLL
                     DEPSLGLAPVLVQEVFKVIKAINKAGTTILLVEQNARMALLIANRWFVLETGRVMLAD
                     TAPNLLNNEEMKAHYLGAHAHVKEARRRRKNERPSA"
     gene            1285..2418
                     /locus_tag="AC16G0_16200"
     CDS             1285..2418
                     /locus_tag="AC16G0_16200"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5101197.1"
                     /translation="MKQIRILSAALVSSLLVMTVSLAYAASGEILIGMDTPVTGANAA
                     NGALWIKAVKLAEKQINAAGGINGKQIKVLINDDQSTNPGALAAVNKSIEQDKALALL
                     GPTTSTQILAVSETIKQAAIPTLVGGSAVAVTRQGNPWLFRVRPDDSIVAQGMVKYIK
                     EDRKLTKVGILHDADAFGSGGADLVEKYAKEAGLTVVKREKYSGADRDFTAQLLSLKS
                     AGAEVMVVYATAGPAIAVIQRQYRQIGSPYKFLGSPTSAQENTLALSKEAAEGLIAMM
                     DNVPGEALKKYAADYRKEYNEDLNTFAAFDYDALMILANAIKKAGEDRAKIREALLAT
                     KSYVGVLGTYSFTPNGDSLHTATVVQIESGKPKLLKVVNVEPK"
     gene            2450..>3016
                     /locus_tag="AC16G0_16205"
     CDS             2450..>3016
                     /locus_tag="AC16G0_16205"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018343078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HIR5101198.1"
                     /translation="MATRILPREFYTRTNVLTIARRLLGTLLVVPAPNGTRVSGIIVE
                     TEAYHGPQDRASHAYGGRRTNRTETMYQIGGTAYVYFVYGMYHQFNVVTNVKDVPHAV
                     LVRALQPVEGIELMRERRHGQPDANLTNGPGKLCIALGIDRALDRVDLLGDRVWIERG
                     GRRMASSAIAAGPRIGIDYAEDWADKPWR"
BASE COUNT          595 a          988 c          950 g          483 t
ORIGIN      
        1 ctggcccggg ccttggtctc agagcccaag ctcctcctgc tggatgagcc ggcggccggc
       61 ctgaacgcgg cggagaccga ggtcctggtg gacctgctct tccagatctg cgaccgggga
      121 atcaccatcc tgctggtcga acacgacatg agcctggtga tgaacgtctc cgatcacatc
      181 accgtgctca acttcggcaa gaagatcgcg gagggctcag ccgaggtcat cgaaaagaat
      241 caagaggtca tcgacgccta cctgggaacg gaggtgggca atgcttaagg tgcaagggat
      301 agaaacctgg tacggggcca tccaggccct caagggcgtc tcgctggacg tgaagcaggg
      361 agagatcgtc gccctgctgg gcgcctacgg tgccgggaag agcaccacca tcaagaccat
      421 caccggccag ctcaagccgg ccaggggccg gatcgagttc acgggccagg acatcacggg
      481 acgcgaggtc gaggacatcg cggaggccgg gatcgcctgc gtgcccgagg ggcggcgtat
      541 cttcgccggc ctctccgtca tggacaacct gatgctgggc gccaccccgc ggaagaacgc
      601 ccccaaggcc gacatccagc gggacctgga tcgcatcttc accctcttcc cgatcctcaa
      661 ggagttcaag gaccggttgg gctggcagct ctccgggggt cagcagcaga tgctggccat
      721 cggcagcggc ctcatgagcg gggccaggct cctcttgctg gatgagccgt cgctcggctt
      781 ggcccccgtc ctggtgcagg aggtcttcaa ggtcatcaag gcgatcaaca aggcaggcac
      841 gaccatcctc ctggtggagc agaacgcccg catggccctt ctcatcgcca accggtggtt
      901 cgtcctggag acgggcaggg tgatgctggc cgacaccgcg cccaacctgc tcaacaacga
      961 ggagatgaag gcgcattacc tgggcgccca cgcccacgtc aaggaagccc gacgccgccg
     1021 caagaacgaa cgacccagcg cctgacgcca gggtgcgcct cccgaggccc ggcggtttcg
     1081 accgccgggc ctttaggttc tccgagtcca tcgcatccga ccgccatcga atccggagtt
     1141 gcccgagacc ttgacagccc ccggccatct ggtctagttt tcaccctcgc ggaacggggc
     1201 tgccgatccc atccgcatcc cgcgcgagtg gccgatccgc tggtctccca agtgttttcc
     1261 cccatgggtg aaaggagaga agtggtgaag cagatacgta tcctgtcagc ggctcttgtt
     1321 tcgtccctcc tggtcatgac ggtcagcctg gcctatgccg ccagcgggga gatcctcatc
     1381 gggatggata cccccgtcac gggcgccaat gcggccaacg gtgccctgtg gatcaaggcg
     1441 gtcaagctgg ccgagaagca gatcaacgcg gccggcggca tcaacggaaa acagatcaag
     1501 gtgctcatca acgacgacca gtccaccaat cccggcgccc tggccgccgt gaacaagagc
     1561 atcgagcagg acaaggcgct ggccctcttg ggcccgacca ccagcaccca gatcctggcc
     1621 gtttccgaga ccatcaagca ggcagcgatc ccgaccctgg tgggcggcag cgccgtggcc
     1681 gtcacccgcc aggggaatcc ctggctgttc cgggtccgcc cggatgactc catcgtggct
     1741 caagggatgg tgaagtacat caaggaagac aggaagctga ccaaggttgg catcttgcat
     1801 gacgccgacg ccttcggctc cggcggggcc gatctcgtcg agaagtatgc caaggaggcc
     1861 ggcctgacgg tcgtcaagcg ggagaagtat tcaggcgccg atcgggattt caccgcccaa
     1921 ctcctctcgc tgaagagcgc aggcgccgag gtgatggtgg tctacgccac cgccggcccg
     1981 gccatcgcgg tgatccagcg gcagtaccgc cagatcggct ccccctacaa gttcctcggc
     2041 tctccgacca gcgcccaaga gaacaccctg gccctgtcca aggaggcggc ggaaggcctg
     2101 atcgccatga tggacaacgt gcccggtgag gcgctgaaga agtacgcagc ggattacagg
     2161 aaggaataca acgaggatct caacaccttt gccgccttcg actacgacgc gctgatgatt
     2221 ctggccaacg ccatcaagaa ggccggtgag gaccgcgcca agatccggga agccctcctg
     2281 gccaccaaga gctacgtcgg cgtcctgggt acctactcct tcacccccaa cggcgacagt
     2341 ctgcacaccg ccaccgtcgt ccagatcgag agcggtaagc ccaagctgtt gaaggtcgtg
     2401 aacgtggagc cgaagtagtc ctggccggca cagttcaccc ttgcctccca tggccaccag
     2461 gatacttccg cgcgagttct atacccggac aaacgttctg acgatcgcgc gccggttgct
     2521 cggcacgctg ctggtcgttc cggccccgaa cgggacgcgt gtctccggga tcatcgtcga
     2581 gaccgaggcc taccacggcc cgcaggaccg cgcctcacac gcctatggcg gtcgtcgaac
     2641 caaccgaacg gagacgatgt accagatcgg tggtaccgcc tacgtctatt tcgtctacgg
     2701 catgtaccac cagttcaatg tggtgaccaa cgtcaaggac gtgcctcacg ccgtgctggt
     2761 tcgcgccctc cagccggtcg aaggcatcga actgatgcgc gagcgccggc atggacaacc
     2821 ggatgccaac ttgaccaacg gcccgggaaa gctttgcatc gccctgggaa tcgaccgggc
     2881 gctggatcgc gtcgatctgc tgggggatcg ggtctggata gaaaggggtg gcaggcggat
     2941 ggcgtcctcg gcgattgccg ctgggccccg catcgggatc gactatgcgg aggactgggc
     3001 ggataaaccc tggcgc
//
LOCUS       DBNGZC010000939         1918 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_862457, whole genome shotgun sequence.
ACCESSION   DBNGZC010000939 DBNGZC010000000
VERSION     DBNGZC010000939.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_862457"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="AC16G0_16210"
     CDS             complement(<1..603)
                     /locus_tag="AC16G0_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR5101199.1"
                     /translation="MRTFERSRPVFWVAVLILLVSTVGCAQLEYAPKGNYLFYHKQLP
                     DADRAVEAARSAGRDRECPDAFREAEKLKNDAYETYWACRTHEAIRMANEATAKANAL
                     CPRVAPAAAVPPPPAPPTVSLSANPAAIVTGQCSTLNWTSTNANTATINQGIGGVTPS
                     GSRQVCPTTTTQYEINATGPGGSRTASTTVAVNAPPPPPPG"
     gene            962..1774
                     /locus_tag="AC16G0_16215"
     CDS             962..1774
                     /locus_tag="AC16G0_16215"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR5101200.1"
                     /translation="MDPSVIPNLITDLLVRYSFQVLAALAILAAGFLCAHWAGKFIER
                     SLRRTQIETHLQRLLVRAGKLLVLAFTVILALDKFGIQVTALVAGISVAGLAGGLALQ
                     GVLANLAAGVSILFSRYFRIGDYIEIGTVKGQVTSIDLSMTVLRTLEDARVIVPNRRI
                     VGEVIYNYSAERRVTLHVEVGYGENLDKALGAVQAVLAENPRVLKDPPPEVGIVHLSD
                     AGIQFALRPWCKAEDFWRVQYEVYRAILERFRQEKIATPFPTREVRLVQTLA"
BASE COUNT          309 a          625 c          609 g          375 t
ORIGIN      
        1 cccggggggc gggggggggg gggcgttcac agccaccgtc gtggacgcgg tccgggaacc
       61 gccaggtccg gtggcgttga tctcgtactg cgtcgtggtg gtcgggcaaa cctgccggga
      121 gccgctcggc gttacgccgc caatcccctg gttgatcgtg gcagtgttgg cgttcgtcga
      181 ggtccagttc agtgtcgagc actgccccgt cacgatcgcg gccgggttgg ccgagagcga
      241 caccgtggga ggggcaggcg ggggcggcac ggcggcggcg ggcgctactc tcggacagag
      301 cgcattggcc ttggctgtgg cctcgttggc cattcgaatc gcctcgtgcg tgcggcacgc
      361 ccagtaggtc tcatacgcgt cattcttcag cttctcggcc tcccgaaacg cgtcggggca
      421 ttcccgatct ctgccggcgc tccgtgccgc ctctaccgcg cgatccgcat ccggcagttg
      481 cttgtggtag aagagataat tccctttggg ggcgtactcg agttgtgcgc acccgacggt
      541 tgaaaccaga agaatgagga ctgccaccca gaagaccggc cttgagcgtt cgaacgttct
      601 catcagtcgc ctcctttgga gatcatttcg ataagaaccg ataacgcaac gggggtttaa
      661 ccatagcatt tactccaatc gaaatccagt cacctattcc tcctaccgga ccgcgaaggt
      721 ctcgaatccg cgggtactgc ggtcgccaac gctcacggtc gtcgtcacct cgcggattct
      781 tgtcgtctcc tcgatgggct ccttttgcat ccccttcttg gtcagacccc caccggtcgc
      841 tcccccttga gcaggtcccg gatctcgccg agcaactctt gctgacttga ccctctcatc
      901 gggcccggtt atcctccgcg cagcattgaa ccgcacgttc ccacttgtga aggagggtgc
      961 catggaccca tccgtgattc cgaacctcat caccgatcta ctggtccgct acagctttca
     1021 ggtcctcgcc gccctggcca tcctggccgc gggattcctg tgcgcccact gggccgggaa
     1081 attcatcgag cggtcccttc gccggacgca gatcgaaacg cacctccagc gtctccttgt
     1141 ccgggcggga aagctcctgg ttcttgcctt cactgtcatc ctggcgctcg acaagtttgg
     1201 cattcaggtg accgccctgg tggccgggat cagcgtggcc ggcctcgccg gcggcctcgc
     1261 cctgcagggc gtcctggcga acctggcagc gggggtgagc atcctgttct cccgttactt
     1321 ccgcatcggg gattacatcg agatagggac cgtcaagggg caggtgacct ccatcgacct
     1381 gagcatgacg gtgctccgta cgctggagga cgcccgggtg atagtaccga accggcgaat
     1441 tgtcggcgag gtcatctaca actactccgc ggaacgacgc gtcacgcttc acgtcgaggt
     1501 ggggtacggc gagaacctgg acaaggcgct cggcgccgtt caggcggtcc tggcggagaa
     1561 ccctcgggtt ctcaaggacc ccccgcccga ggtcggcatc gtgcacctct cggacgcggg
     1621 gatccaattc gccctccggc cctggtgcaa agcggaggat ttctggcggg tacagtacga
     1681 ggtgtaccgc gcgatcctgg agcgcttccg ccaggagaag atcgcgaccc cgttcccgac
     1741 gcgtgaggtg cgcctggtgc agaccctggc gtgatcttcg ggacaggccg ccacgagcgc
     1801 tcgtgcctgc cgggaccttt tgacaacccg gcggaaaggg gagggaaatg ttctcgcgcc
     1861 ggtccgtcga gaccggcatg actcctgcca gagctcgaac cggagcgccc atcatgag
//
LOCUS       DBNGZC010000940         1973 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2427421, whole genome shotgun sequence.
ACCESSION   DBNGZC010000940 DBNGZC010000000
VERSION     DBNGZC010000940.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2427421"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1870
                     /locus_tag="AC16G0_16220"
     CDS             185..1870
                     /locus_tag="AC16G0_16220"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5101201.1"
                     /translation="MPSDALSFPLPTRRNLEDRAATRLLPWALGLLLVGSLLLFIAYP
                     VLTVLARSVAAKGGGLTLSHFQTFFAKAYFLRSLSNTLLLGVSVTLLVSALGFALAYL
                     VTRGPGPLRLPLRAITVAPLVAPPYLFGLALIIIAGRRGFLSQLLGVQIPIYGWPGVI
                     LAQILSLLPVAFLMIENVLLTMDPNVEDSASDLGAGETTLLRTIILPLATPGLLKAGL
                     LTFGLSIADFGNPALLGGRVSFLATDAFLMVTGDWNMEMASAISVILLVPSLAIFLAQ
                     HYWLRGKTFVTMGGAPVAAEERRIQPWLQVGLWIPCLLYATAVVASYGVIVLGAFTKL
                     VGIDNRFTLEHLVTDVGLDAIWTSMKVSLTAGVFAALVGTGLAYLCVRGQSRLRGALE
                     FVTLLGFALPGTIMGIGYLLAFNRPPLLLTGTLFILILNMIARFLAVGLEAGVSKLHQ
                     IDISIEEASADLGAGILTTFRRIVLPLMSSAFVAGFLFTFMQSMISLSAAIFLVAPGR
                     LLASVYIFNRARDGDMGIACAMSVILIASVAVCLLGLSFLAKRTGMTIFAART"
BASE COUNT          276 a          709 c          603 g          385 t
ORIGIN      
        1 cagcgctgga tcgcgcagta cggctacctc tcggcccgcg acatgcccaa tccgatgtac
       61 acgacaaagc ccaagttcat cgagtactcg tacgagtggg cggcggacaa ccacgcccgc
      121 gtcatcgaga cctggaagtc caagttcctg cagaagtagg cacccactcc aaccggaacc
      181 gtgcatgccc tctgacgcgc tgagctttcc cctgccgacc cggcggaacc tggaggaccg
      241 ggcagcgacc cgactgctgc cgtgggcgct cgggctcctc ctcgtggggt cgctcctgct
      301 cttcatcgcg tacccggtgc tgaccgtcct ggcccggagc gtggcggcaa aggggggcgg
      361 gctcaccctc tcccatttcc agaccttttt cgcgaaagcc tactttctcc gctccctgtc
      421 caacacgctc ctcctcgggg tgagtgtgac gctgctggtg tcggcgctcg ggttcgcgct
      481 ggcgtacctg gtgacccgcg ggccgggacc gctccgcctc cccctgcggg ccatcacggt
      541 cgcgcccctc gtcgccccgc cctacctctt cggcctggcc ctgatcatca tcgccggccg
      601 gcggggcttt ctgtcccagc tgctgggcgt ccagatcccg atctacggct ggccgggcgt
      661 cattctcgcg cagatcctgt cgctgctgcc cgtggccttc ctgatgatcg agaacgtgct
      721 cctcaccatg gacccgaacg tcgaggacag cgcaagcgac ctgggcgccg gggagacgac
      781 gctgctccgg acgatcatcc ttcccctagc gacgccgggt ctcctcaagg ccggactgct
      841 cacctttggg ctgtctatcg cagacttcgg gaatcctgcc ctgctgggag gccgagtctc
      901 gttccttgcc accgacgcct tcctgatggt cacgggggac tggaacatgg aaatggccag
      961 cgccatctcc gtgatcctcc tggtcccgag tctggcgatc ttcctggccc agcactactg
     1021 gctgcggggc aagaccttcg tcaccatggg gggcgccccg gtcgccgccg aggaacggcg
     1081 catccagccc tggctgcagg tcgggctctg gatcccttgc ctgctgtatg cgacggcagt
     1141 cgtcgcctcg tacggggtga tcgtcctggg ggcgtttacc aagctggtgg gcatcgacaa
     1201 ccggttcacc ctggagcacc tggtgaccga tgtcgggctc gacgccatct ggaccagtat
     1261 gaaggtctcc ctcaccgcgg gtgtcttcgc cgccctggtc gggacgggcc tggcctatct
     1321 ctgcgtcagg gggcagtcgc gcctccgcgg cgcgctggag ttcgtcaccc tgctcggttt
     1381 cgccctcccc gggaccatca tgggcatcgg ttacctgctc gccttcaacc ggccgccgct
     1441 cctgctcacc gggacgctct tcatcctgat cctgaacatg atcgcccggt tcctggccgt
     1501 gggcctggag gcgggggtga gcaagctcca ccagatagac atcagcatcg aggaggcttc
     1561 ggccgacctc ggggcgggga tcctcaccac attccgccgc atcgtgttgc cgctgatgtc
     1621 ctcggccttc gtggctggat tcctgttcac gttcatgcag tccatgatca gcctgagcgc
     1681 cgcaatcttc ctggtggccc ccgggcgact cctggcctcc gtgtacatct tcaaccgcgc
     1741 ccgcgacggg gacatgggga tcgcctgcgc catgtccgtt atcctgatcg ccagcgtggc
     1801 cgtctgccta ttgggcctgt cgttcctggc caaacggacg ggcatgacga tctttgccgc
     1861 ccgcacctga ccgcgtccgg aggagccgaa tgcctgccca caccctgttg agcctggatg
     1921 gcctcaccaa acgctacgac accgtcatcg ccgtcgaggg agtctgcgcc gcg
//
LOCUS       DBNGZC010000941         4262 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1054708, whole genome shotgun sequence.
ACCESSION   DBNGZC010000941 DBNGZC010000000
VERSION     DBNGZC010000941.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4262)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4262)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4262
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1054708"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="AC16G0_16225"
     CDS             <1..382
                     /locus_tag="AC16G0_16225"
                     /inference="COORDINATES: protein motif:HMM:NF016027.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5101202.1"
                     /translation="LLRRAALSISQAGYNTLLDVVSSRVRAVVVPYEGSGDEQRLRAE
                     LLAERGLVKVVSEEALSPARLAAAMQEVLTAPDFPALARLDLHGASRSVTILRELVDE
                     VMAARKPRRSAARPGGRRQPARRR"
     gene            complement(361..870)
                     /locus_tag="AC16G0_16230"
     CDS             complement(361..870)
                     /locus_tag="AC16G0_16230"
                     /inference="COORDINATES: protein motif:HMM:NF024104.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5101203.1"
                     /translation="MSRLDSHIQQKIAQRDSINLAARWLTGQKGVIVEFGLGSGRSYS
                     HLLERFPGREIFCFDRTEKAHPRSRPPASHLILGEFTDLLVDPALHARFAGGVILAHI
                     DIGSGGPEDEVMPELLISRIHPWLRPEAAVLSDQDLTLQPAWQLERVDTTGQVEYAER
                     YYVYRRRAG"
     gene            977..1819
                     /locus_tag="AC16G0_16235"
     CDS             977..1819
                     /locus_tag="AC16G0_16235"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR5101204.1"
                     /translation="MSLDQNSGGVLAQAWDESAWNALAGELDAWLGQRHQATLWWRDD
                     DAGRPHPALEQLLTAGGRMGIPLGVAVVPAWLTPEVAETLEAAGEIVVVLQHGFAHAN
                     HETEIQPGERKVRPAECGVGRSVGVALEELTVGATRLRARFGGRFLPLFVPPWNRIAP
                     AVIAELPRLGYRGLSTFGPRQVATPAPGLLQVNCHVDPILWRQGKRFAGPTVTLDRLR
                     AHLSARRERRVDPSEPTGILTHHRDLDPAGWAFLEELLSRLRTHPAATFPSLASLLTD
                     GTEG"
     gene            complement(1914..2588)
                     /locus_tag="AC16G0_16240"
     CDS             complement(1914..2588)
                     /locus_tag="AC16G0_16240"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR5101205.1"
                     /translation="MSEPTRHDPPYEIATEVLYSVANSPSTQPPQKAKGMCARNLSQG
                     GLLLEAQSRLSPGTRLTLLLIRENRGAIDAQGEVVWADEAAGQATFRHGIRVVRLEPS
                     QELAWRSFLDEASRELGRRGHRYDIDVPMTCCRKDNGDNLGGRAIAVNVSRGGFLVLL
                     PVRVPVDTILCLEVRTATQSLRTEARVVRLEEPRPDGLIPHGLAFVDSDEGSRLLPEL
                     FLVGIL"
     gene            complement(2786..3541)
                     /locus_tag="AC16G0_16245"
     CDS             complement(2786..3541)
                     /locus_tag="AC16G0_16245"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR5101206.1"
                     /translation="MAHWLATEGFTRLSVLEGGLSAWRKAGFPTDMLQGEAPVATDGR
                     GSAESEITRPESFLPSLATRYLTNGGLPTRRRLTTLFVDIAGSTRLVEQHPAEAVLAL
                     VQRFMHLVTDVALAYCGDVKDFEGDGALLYFESTSEASQAALAIRDAIASGVCATVCP
                     VSARMSLTVGQVVVGLVGTTMRRSIALIGESVHVGARLLKHVRPGGIIASGEFVQSLR
                     AERSPLADQFRLQDQAFEIPGADAITVTTYVIL"
     gene            complement(3566..3787)
                     /locus_tag="AC16G0_16250"
     CDS             complement(3566..3787)
                     /locus_tag="AC16G0_16250"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR5101207.1"
                     /translation="MPTSMDDVERITAHELNSRLLKGEPIAVLDVRRREAWATDPDHI
                     PGAIWVPLEEADQRARDLPPDTHIVLYCS"
     gene            complement(3908..>4262)
                     /locus_tag="AC16G0_16255"
     CDS             complement(3908..>4262)
                     /locus_tag="AC16G0_16255"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR5101208.1"
                     /translation="PAGGGTERLPRLIGESRARELIYTGEPVDAREAWRIGLVNRVAP
                     AGQALAGAQELGRTIAARSGLTLRTVKSVMDRGLCMDLLEAQQVAIDAIAELFQSEAV
                     REGVTAFLEKRPPRF"
BASE COUNT          718 a         1407 c         1435 g          702 t
ORIGIN      
        1 cctcttgcga cgggccgcgc tctcgatcag ccaggccggc tacaacacgc tgttggacgt
       61 ggtgtcgagt cgggtgaggg cggtggtggt cccctacgag gggagcggcg acgagcagcg
      121 gctgcgcgca gagcttctgg cagagcgggg gctggtcaag gtggtctccg aggaggccct
      181 ctcgccggcc cgcttggctg cggccatgca ggaggtgctg accgcgcccg atttcccggc
      241 gctggcccgg ctggatctcc atggggccag ccgttcagtc acgatcctgc gagagctggt
      301 ggacgaggtg atggccgccc ggaagccacg caggtccgcg gcgcgccccg ggggccgacg
      361 tcaaccagcc cgtcgccggt agacgtagta gcgttcggcg tattcgacct gacccgtggt
      421 gtccacccgc tcgagctgcc aggccggctg cagcgtcaag tcctgatcgc tcagcaccgc
      481 ggcttcgggc ctgagccagg ggtggatccg acttatgagc agctcgggca tgacctcgtc
      541 ttcgggcccg ccagagccaa tgtcgatgtg tgccaggatc acgccgccgg cgaagcgagc
      601 gtgcagggcc ggatcgacca gcaggtcggt gaactcgccg agaataaggt gggaggcagg
      661 cggtcgggat cggggatggg ctttttccgt gcggtcaaaa caaaagatct cacggccggg
      721 gaaccgttcc aggaggtgcg agtaagatcg cccgctgccg agtccgaact cgacgatgac
      781 acccttctgc ccggtcagcc atctcgcagc cagattgatg ctgtcgcgct gggcgatctt
      841 ctgttgaatg tgggaatcga gtcggctcat gctccgtctc ctctggcggt ggggcggggt
      901 cactcggcac gcgtgatcac tatagcatgg ccctccaggc aacaccattt cctcctgcca
      961 aggagaagcc agtccaatgt ctttggatca gaactccgga ggggtgttgg cccaggcatg
     1021 ggatgagtct gcctggaatg ccctggctgg ggagctggat gcatggctcg gccaaaggca
     1081 tcaggcaacc ttgtggtggc gggatgacga tgccggtcgg ccccatccgg ctttggagca
     1141 actcctcacc gcgggcggga gaatgggcat ccccctgggg gtggccgtgg tacccgcatg
     1201 gctgacgccc gaggtcgcgg aaacgctcga ggccgcaggg gagatcgtgg tcgtgctcca
     1261 gcatgggttt gcccacgcca accacgagac ggaaatccag cccggcgagc ggaaggtgcg
     1321 tccggcggag tgtggcgtgg gccgatccgt cggggtcgcg ctcgaagagc tgacagtggg
     1381 tgcgacccgc ctgcgtgccc gcttcggggg tcgtttcctg ccattgttcg ttccgccgtg
     1441 gaaccggatc gcccctgcgg tgatcgccga actaccgcgc ctggggtatc gcgggctctc
     1501 gacgttcggg ccccgtcagg tggccacgcc tgcgcccggc ctcctccagg tcaattgcca
     1561 cgtggatcca atcctgtggc gtcaagggaa gcgattcgcc gggccgacgg tcaccctcga
     1621 ccggctccgg gcgcatctgt cggcccgccg ggagcgccgg gtggatccat ccgagcccac
     1681 gggaatcttg acgcatcacc gcgacctgga tcctgcaggc tgggccttcc tcgaagaatt
     1741 actcagccgg ttgcggacgc accccgcggc caccttccct tccctggcgt cccttctgac
     1801 cgacgggacc gaaggctgaa cccacctgtc ttaccgcgaa ggtcgcaaag gacgcaaagg
     1861 cagacatggc ggcaggattc ggtcccggga tacagtcgag tcgcgaggaa gggttaaagg
     1921 attcccacga ggaacagttc gggcagcagc cgtgacccct cgtccgaatc gacgaaggcg
     1981 aggccgtggg ggatcagtcc gtccggcctg ggctcctcga gcctgacgac ccgagcttcc
     2041 gtcctgaggc tttgcgtcgc ggtccgaacc tccaggcaga gaatcgtatc cacgggcacg
     2101 cgcacaggca gcagcacgag gaatccgccc cggctgacgt tgaccgcaat ggcacgcccc
     2161 cccaggttgt cgccattatc cttccggcaa caggtcatgg gaacgtcgat gtcatagcgg
     2221 tggcctcggc gaccgagttc tcgactcgcc tcgtcgagga aggacctcca ggcgagttcc
     2281 tgcgacggct ccagccggac gacccggatc ccatgacgaa atgtcgcctg gccagcagcc
     2341 tcatcagccc acaccacctc gccctgggcg tcgatggctc cccgattctc tcggatgagg
     2401 agaagcgtca gacgagttcc gggggacaga cgcgactgag cctccagcaa gagcccaccc
     2461 tggctcagat tccgggcaca catccccttg gccttctgtg gcggctgggt gctgggcgag
     2521 ttcgccacgc tgtacaggac ctctgtggca atctcgtacg ggggatcgtg tcgagtcggc
     2581 tcgctcatcc agtctcgacc cttcctggag cttcgttcag cagagatcgg cgggggacac
     2641 ggacgatcca cgggatgcta tcacccgggt cgccggtgtc aagttcggag tgggtcaagg
     2701 attgagcctc ggtcaggaga cggcgccggc tcgccggtat gcgccatccg cggagcgcgc
     2761 caggaaggca gcccacgcgg ccccgtcaaa ggatgacata ggtcgtgacg gtgatggcat
     2821 ccgctccggg gatctcgaac gcttgatcct gaagccggaa ttgatcggcg agcggagagc
     2881 gctcggcacg gagtgattgg acgaactccc cgctggcgat gattcctccc ggtcggacat
     2941 gcttcaggag ccgtgcaccg acgtggacgc tctccccgat cagcgcgatg gaccggcgca
     3001 tggtcgtgcc caccaacccc accaccacct gacccacggt gagactcatg cgcgcggaga
     3061 cagggcagac cgtagcacag acgcccgagg cgattgcatc ccggatggcg agtgctgcct
     3121 gggacgcctc ggacgtggat tcaaagtaga gcagggcgcc gtccccctcg aagtccttca
     3181 cgtccccaca gtaggcgagg gccacgtccg tcaccaggtg catgaaacgc tgcaccagcg
     3241 ccagcaccgc ctccgcggga tgctgctcca ccaggcgagt ggagcccgcg atgtccacga
     3301 agagcgtcgt cagccgccgg cgtgtgggga ggccgccatt ggttagatag cgggtggcaa
     3361 ggctcggaag gaacgactcg ggtctcgtga tttccgactc ggcgctcccc cggccgtccg
     3421 ttgcgaccgg tgcttcgccc tgcagcatgt ccgtagggaa tccggccttg cgccacgccg
     3481 aaagccctcc ctcgaggacc gagaggcggg tgaacccttc cgtggccagc cagtgtgcca
     3541 cacgggcact ggttgtctcg ccgagtcagg agcagtagag cacaatgtgg gtgtccgggg
     3601 gaaggtcgcg ggcacgctgg tcggcctcct ccaacggcac ccagatcgcc cccggaatat
     3661 ggtccggatc ggtggcccag gcctcgcgcc gccgcacatc tagcaccgcg atgggttccc
     3721 ccttgagcaa tcgggaattc aactcatgcg ccgtgatccg ctcgacgtca tccatggaag
     3781 tcggcatgtg gtctccttcg aaagaagccc ttgatgtatc cccgcctagc agggattcgt
     3841 gcgaattccc aagcgagtga accgggtgcc atgcgtcccg gcgggacggc ccacgggccg
     3901 gggtatgtca gaacctcggc gggcgctttt ccaggaacgc cgtcacgccc tcccggacgg
     3961 cctcggactg gaacaattcg gcgatggcat cgatggccac ctgctgcgcc tccaagagat
     4021 ccatgcagag cccgcggtcc atgacagact tcaccgtgcg gagcgtcagc cccgagcgcg
     4081 cggcgatcgt ccggcccagc tcctgcgcac ccgccagggc ctggcccgcg ggggccacac
     4141 ggttgaccag cccgatccgc caggcctcgc gggcatccac cggctcgccg gtgtagatca
     4201 actcgcgggc gcgggactct ccgatgagtc gcggcaaccg ctccgtcccc ccgccagccg
     4261 gg
//
LOCUS       DBNGZC010000942         1594 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_82186, whole genome shotgun sequence.
ACCESSION   DBNGZC010000942 DBNGZC010000000
VERSION     DBNGZC010000942.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_82186"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="AC16G0_16260"
     CDS             <1..397
                     /locus_tag="AC16G0_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HIR5101209.1"
                     /translation="RFRNRPRREYLVLNDGSHWDVPKGHIEAGEDPETTARRELQEET
                     SLRDARFIPGFKESMRYFYRKAGEDMRKVVIYFLAETAVGDVTLSHEHCGYQWLPYEE
                     TLKRLTFKNARDLLTRAERFLQSGAASTG"
     gene            433..1266
                     /locus_tag="AC16G0_16265"
     CDS             433..1266
                     /locus_tag="AC16G0_16265"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5101210.1"
                     /translation="MQTDRASTMPIASTNGITTYYETCGAGPPLVLLHNDALNLGVWQ
                     RLLPHLALSFRIIAYDRRGHGQTEIPPPEEPYTVDVLADDLRALLDTLGVGSARLFGC
                     SGGANVALAFTLAHPDRVSHLILAEPPIMGLWNDPPIDTGGLSSETAARIMRERDLEA
                     GLDYWFRTVLSPGKAKALLRGHYGPLLLSRPTWLILAILRSAEGFDPGPRLSEVRQPV
                     FLLQGGKSRRLFSDVMDILEVRLPRARRLVLPGVDHAGLLTPGDALLSAVHSFLSGEA
                     A"
BASE COUNT          281 a          554 c          475 g          284 t
ORIGIN      
        1 ccggttccgc aatcgcccgc gacgcgaata cctggtcctc aacgacggct cccactggga
       61 tgtccccaag gggcatatcg aggccggaga ggatcccgag acgaccgcgc gtcgtgagct
      121 ccaggaagag acgagcctcc gcgacgcccg attcatcccc ggcttcaagg agagcatgcg
      181 ctacttctat cgtaaggcgg gcgaggacat gcgcaaggtg gtgatctact ttttggccga
      241 gacggccgtc ggggacgtca cgctgtccca cgaacactgc gggtaccagt ggctccccta
      301 tgaagagacc ctgaaacgct tgaccttcaa gaatgcccgc gacctgctca ccagggccga
      361 gaggttcctc cagtcggggg cagccagcac cggctgaggc ggcaccggtt cccccccctc
      421 ttgccgcaaa ccttgcaaac ggacagagct tcgaccatgc ccatcgcctc caccaacggc
      481 atcaccacct attacgaaac gtgcggggcc gggcccccgc tggtgctgct tcacaacgac
      541 gcgttgaacc tcggagtgtg gcagcgcctc ctcccgcatt tggctctttc cttccggatc
      601 atcgcgtatg accgccgggg ccacggccag accgagattc ctccgccgga ggagccgtac
      661 accgttgatg tcctggccga tgacttgcgc gccctgctgg ataccctggg tgttggatcg
      721 gcgcgtctct tcggctgctc agggggagcc aatgtggctc tggccttcac gctggcacat
      781 cccgacaggg tgagccatct gatcctggcc gaaccgccga tcatgggcct ctggaacgac
      841 ccgccgatcg acaccggcgg cctctccagc gagaccgccg cccggatcat gcgcgagcgg
      901 gacctggaag ccggcctgga ttactggttc cgcaccgtgc tctcacccgg caaggccaag
      961 gcactcctgc gggggcacta tggcccgctc ctcttgtccc gcccgacctg gctcatcctg
     1021 gccatcctcc ggtcggccga aggcttcgat ccggggcctc gtctgtcgga agtgcgccaa
     1081 cccgtcttcc ttcttcaggg cggcaagagc cgccggctct tctccgatgt gatggatatc
     1141 ctggaggtgc gcctgccccg tgcacgacgt ctcgtcctcc ccggggtgga tcatgcgggc
     1201 ctgctcactc cgggcgacgc ccttctctcg gccgtacatt ccttcctatc gggcgaagcc
     1261 gcctgagccc ggctttcaat cgctcgtccc cccacccgcg gggattggcc cgcgcaaaat
     1321 cactgaaacg cgaggatacg tctcccggag gtgccactga tccacctgcc agcgggtgat
     1381 gcagaggcat agccggcagc cgcactgttc cgatgccagc cgcagtgggc acggtcacca
     1441 gtgagaattt ggccttggtg ctacggaagt caggagggaa accggtgggt cctcaagatc
     1501 tacctgcaga agtggaatca gatgacgagc ccctgcctgg actatagcga gctaactctg
     1561 agccgaaaat cggcatctcg ccaagatcac ccaa
//
LOCUS       DBNGZC010000943        11711 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1351219, whole genome shotgun sequence.
ACCESSION   DBNGZC010000943 DBNGZC010000000
VERSION     DBNGZC010000943.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 11711)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11711)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11711
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1351219"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="AC16G0_16270"
     CDS             complement(<1..622)
                     /locus_tag="AC16G0_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018206494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5101211.1"
                     /translation="MPNDALLQVRNLQKHFEIKRGFFWGEPAKVHAVDGVSFDLFRGE
                     TLGLVGESGCGKSTTGRLILRLIEPTAGTVTFENVSVFQAGKAEMRAMRQRMQIIFQD
                     PYSSLNPRMTVEQIVGEGIVIHKLCKTKAERRERVADLLQKVGLSPEQMHRYPHEFSG
                     GQRQRVGIARALAVNPKLIIADEPISALDVSIQAQVINLLEDLQEQF"
     gene            complement(634..1626)
                     /locus_tag="AC16G0_16275"
     CDS             complement(634..1626)
                     /locus_tag="AC16G0_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008945452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5101212.1"
                     /translation="MDQTTASKNGVLLEINGLQTNFFTDAGVVRAVDGVSLKVRKGET
                     LGIVGESGCGKSVTALSILRLIPDPPGKIVAGKILLDGRNLLDLPENEMRKVRGASIS
                     MIFQEPMTSLNPVFTVGDQIAEGIRLHQHLSKHDSWNKAIEMLRLVRIPDPERRVKEY
                     PHQMSGGMRQRVMIAMALSCNPNLLIADEPTTALDVTIQAQILDLLNQLKAELGMAVM
                     LITHDLGVVADTAARVAVMYAGRVVEEAPVMELFTNPLHPYTQGLLASIPRIEKAERR
                     PRLQAIPGMVPDLLDLPRGCKFQARCSKIFEPCRGEEPQLKQIKSDHFVRCFLY"
     gene            complement(1743..2642)
                     /locus_tag="AC16G0_16280"
     CDS             complement(1743..2642)
                     /locus_tag="AC16G0_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5101213.1"
                     /translation="MTSRERRAGSGDGRQASSASPWHDALRRLQRNKLAMLGLGVLAC
                     LVLSGLAAPWISPYPYDRTDLRYGAKPPSGTHLLGTDELGRDLLTRILYGARISFAVG
                     ILASVVSLTIGVSYGSVAGSVGGRLDHLMMRVVDIMYGLPFMFFVIILMVIFGRSILN
                     LFIALGAVQWLTMARIVRGQVMSLKVREFVLSAQALGASASRIVTRHLLLNALGPIVV
                     YATLTVPAVMLEEAFLSFLGLGVQPPMASWGSLASEGAAAMETYPWLIVFPGLALTLT
                     LLSLNFLGDGLRDALDPQVTARR"
     gene            complement(2639..3556)
                     /locus_tag="AC16G0_16285"
     CDS             complement(2639..3556)
                     /locus_tag="AC16G0_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013969919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5101214.1"
                     /translation="MLGYIVRRVAGFVPVLFVIVSLAFFMMRLAPGGPFDQERVLPEQ
                     VRANIEARYHLDEPLSRQYLRYLGDVLRGDLGPSFRYPDRSVNELLGLGFPVSLSLGL
                     CGLAVAVLLGGTSGIVAGLRRNTCLDYLTMSFALGGVSVPNFVLGPVLMLVFALWLGW
                     LPVAGWGTWRHLVLPSLTLGTFYAAYVARLTRAGMLEVIGQDFVRTARAKGLREAVVV
                     LRHALPSAILPVVTYLGPASAAILTGSVVVETIFSIPGIGRYFVGSALNRDYTMVLGT
                     VVFYSVLLLLLNLVVDVLYAYLDPRVQVQ"
     gene            complement(3564..5132)
                     /locus_tag="AC16G0_16290"
     CDS             complement(3564..5132)
                     /locus_tag="AC16G0_16290"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5101215.1"
                     /translation="MKPMGHMLAILLLGAAVAAPAAGAEGQVLRINNMVEPESLDPGV
                     VTGVPEHRILSNLFEGLTTADPKDLSPRPGMATSWTVSKDGLVYTFRLREARWTDGKP
                     VTAHDFVYAWERALNPKFGAKYAQQLFYLKNGEDYNKGRITDFSQVGVKALNDLTLQV
                     TLRCPTAYFLDLTSFYTLYPVPRWAVESYGKDWVKPGKIVTNGPFRLASWVPQQELVL
                     EKNPQHWDASRVKLQKVVFLPTEDVNTAYKQFLAGESDWVPQVPTAQTEAARSRPEFY
                     VTPYLGTYYLRVNVTKPPTSDIRVRKALSLAVDREALTKYVTKAGQIPTSTFVPAGMR
                     GYEGARGLGFDAAAARKLLAQSGYPDGKGLPKIELLYNTNELHRVVTQAVQQMWKDSL
                     GIQVDLANVEWKVYLARQSTLEYQIARAGWIGDYVDPNTFLDMWVTGGGNNETGWSNK
                     RYDDLIAQAACKIVNPKERMHALQTAEQILVDEAPVLPLYTYVNTGMLSRRVKGWYPN
                     ILDQHPLKYISLEQ"
     gene            complement(5211..6125)
                     /locus_tag="AC16G0_16295"
     CDS             complement(5211..6125)
                     /locus_tag="AC16G0_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5101216.1"
                     /translation="MQQSIPASSTIELPLESRSGTRGRSPLGAAVHRLLANHVARGGL
                     VIIAVFVLMAVLSPVLLPYNPQLDSDLLARLKPPSGTHPFGTDALGRDILMRIFHGAR
                     VSLGLGVSSVLLAGLIGSVLGLLAGYAGRWVDLCLMFCMDILLAFPATLLAIAIVAMI
                     GPGLRNSLIAISVVSIPFYARITRGEVLALKEREFVTAARGLGGSPHWIVLWHIFPNT
                     LPPLAVQTTLAVAFAILEAAALGFLGLGAQPPTPEWGAMLADSYKFFTSGAWWAFFFP
                     GAAIMLSVLGFNLVGDALRDALDPRMRV"
     gene            complement(6091..7095)
                     /locus_tag="AC16G0_16300"
     CDS             complement(6091..7095)
                     /locus_tag="AC16G0_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005488866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5101217.1"
                     /translation="MGRYAITRFLTVVPVLVGVSLIVFSFTHLIPGDPAVAMLGERAT
                     PQRVAEVRAQLGLDQPLYQQYFIYVGKILRGDLGASILRGDPVLRDLVRRFPATVELA
                     TSAILIAILVGVPMGIVSAVWRNSFFDGFSRLVALTGVSMPIFWLGLMLAWCFGVVLG
                     WLPTGFRLGTDVTLVAVTHFNILDSLLRRDPAALLSSLRHLVLPAIALSTIPMAVIAR
                     MTRASLLEVLSQDYIRTAQSKGLPQRAVILRHAMRNALLPVMTVTGLQVGRLLAGAIL
                     TETIFSWPGIGLWIYESIQARDYPIVQGATLFIATIFVMVNLITDVLYATVDPRIKYD
                     "
     gene            complement(7127..8746)
                     /locus_tag="AC16G0_16305"
     CDS             complement(7127..8746)
                     /locus_tag="AC16G0_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5101218.1"
                     /translation="MDRMLRILSFILLAGLVCWGGPALAQTTFIFGGQGEPVDLNPAI
                     ITDGISSRITRQIYEGLTKYKGATTEVIPALAEKWQVSSDGTVWTFTLRKNVKFHDGT
                     PFDAAAVVWNFDWQRLSAQPQHEMLTKAGRTFEYYGAQFGGFDDKSLITKVEAVNPST
                     VRFTLKSPQGPFLANLAMFTFDLVSPTAVQKWGLEFSKHPVGTGPFKFVEWKVGQEVV
                     LEANKNYWNKAEAPKVQRVIVRNIKDNSQRLAALKAGEIHGMEGVNPDDVKVVKADPN
                     LQLILRPTNTTGYVAFNFKVKEFQDGRVRQAFAQAINKKAIVDALYGGTGMVATQFQP
                     PPLWGHNHELKDYEYNPQRARELLKDAGFGQGLKDITWDDGRKEQLQFWYMPVSRPYY
                     PNPKEIAEAIAADLAKVGISVQLQTTDWTVYLDKSRKGQVPLWMLGWTGDNGDPDNFV
                     CYFFCAYGEPRMGFYANRALTDTLLEAQKLTDRGKRATLYRKAEQMIHDDAARIFIAH
                     NQPPLAFLKKVKGYVANPTSSEYFNTVSIQP"
     gene            complement(8776..9969)
                     /locus_tag="AC16G0_16310"
     CDS             complement(8776..9969)
                     /locus_tag="AC16G0_16310"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR5101219.1"
                     /translation="MTLSQRALNLVPSATLAISATARRMRAGGIDVISFGAGEPDFDT
                     PDHIKEAGIQAIRDGFTKYTATGGIDELKDAVASKLKRDDGLTYARNQVLISCGGKHA
                     LYNVFQAMLDPGDEVIIPAPYWVSYPEMVRLCGATPVFVQTSEQEGFRLSRELLEQAV
                     TPHTKMLVLNSPSNPTGAAFTRKELEGLAALAVERGVWILSDETYEQLVYDGYRHVSI
                     ASLGSQAYARTILVNSLSKAYAMTGWRVGYTAGPAEIIAAMDGLQSQMTSNPTSVSQR
                     AAVAALLGPQEPTHRMREEFARRRRYIVDRLNAMPEVRCLNPEGAFYVFPNVSGMYGK
                     SFADTVVTNSTKFSAFLLEAARIAVVPGVEFGSDAHVRISYATSMGNIEKGMDRMASA
                     LGQLR"
     gene            complement(9966..10475)
                     /gene="coaD"
                     /locus_tag="AC16G0_16315"
     CDS             complement(9966..10475)
                     /gene="coaD"
                     /locus_tag="AC16G0_16315"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR5101220.1"
                     /translation="MQVTAVYPGTFDPFTNGHIDLVERARRIFTHLIVAVAGNPHKNP
                     LFPLEERIAIVRQATAGMPGVEVDSFQALTVDYVLSKGAQVILRGVRAVSDYEFEFAM
                     ALMNRKMSHQVETVFLMPNQAYTYLSSRLVKEVALLGGNVDDLVPPLAAKLLKERASQ
                     LPHLGRRTT"
     gene            complement(10466..11029)
                     /gene="rsmD"
                     /locus_tag="AC16G0_16320"
     CDS             complement(10466..11029)
                     /gene="rsmD"
                     /locus_tag="AC16G0_16320"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR5101221.1"
                     /translation="MRISAGLHRGRHLQSPKGLRTRPTSEFLRQAIFNMIGERIRGAV
                     VLDLYAGTGAIGLEALSRGAAGATFVERDRRAVASLRANLTALDLGARSRILLGEVLP
                     ILGELQEEGETFDCVFLDPPYAADQAVGCIETLAPGGILSENALLVVQAFHKTALPGQ
                     AGVLRRSDQRRYGESALTFYVRESPCK"
     gene            complement(11063..>11711)
                     /locus_tag="AC16G0_16325"
     CDS             complement(11063..>11711)
                     /locus_tag="AC16G0_16325"
                     /inference="COORDINATES: protein motif:HMM:NF012493.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIR5101222.1"
                     /translation="YVVCPVVEESAAELKAALQTAEAYRLGPLARFGVGLVHGRLRAG
                     EKLQAVADFREGRVRLLVATTVVVVGMDVPEATAMVIEHADRLGVAQLHQLRGRVGRS
                     SCPAVCFVVADRHEISEEARARLEVFVREGDGFALAEADLRLRGPGEFFGTRQAGLDG
                     FQLGDLGADLRLLEDARAEAMAVLAHSPELDGEWSAVRAAMEQRWKSRLGLAQVG"
BASE COUNT         2107 a         3902 c         3705 g         1997 t
ORIGIN      
        1 tgaactgttc ctggagatcc tccaggaggt tgatgacttg ggcctggatc gagacatcca
       61 gcgccgaaat cggctcgtcc gcgatgatca gcttgggatt gaccgccagg gcccgcgcga
      121 tgcccacacg ctgccgctgg ccgccgctga actcgtgcgg gtagcggtgc atctgctccg
      181 ggctcaggcc caccttctgt aggaggtccg ccacgcgttc acgtcgctcc gccttggtct
      241 tgcagagttt gtggatcacg atgccttcgc ctacgatctg ctcgaccgtc atgcgcggat
      301 tcagtgagga gtacggatcc tggaagatga tctgcatccg ctggcgcatg gcccgcatct
      361 cggccttccc ggcctggaag acagacacgt tctcgaaggt caccgtgccc gccgtgggtt
      421 cgatgaggcg gagaatcagt cgccccgtgg tggacttgcc gcagcccgat tcccccacca
      481 ggcccagggt ctccccgcgg aagaggtcga agctgacccc gtccaccgcg tggaccttgg
      541 ctggctctcc ccagaaaaat cctcgcttga tctcaaagtg cttctgcagg ttccggacct
      601 ggagcaaggc atcattgggc atgcgggttt ccttcaatat agaaagcagc gcacgaagtg
      661 atccgacttg atctgcttca actggggctc ctcgccccgg cacggctcga agatcttgct
      721 gcagcgcgcc tggaacttgc acccgcgcgg gagatccagg aggtccggaa ccatgccggg
      781 aatggcctgg agccgcggcc ggcgttccgc cttctcgatc cgggggatgg aggccagcag
      841 cccctgggtg tacggatgca gcgggttcgt gaagagttcc atcaccggcg cctcctccac
      901 cacccgtccc gcgtacatca ccgccacccg ggcggcggta tccgccacca cgcccaggtc
      961 gtgggtgatg agcatgaccg ccatccccag ctcggccttg agttggttca gcagatccag
     1021 gatctgggcc tggatggtca cgtccagggc cgtggtgggt tcgtcggcga tgagcaggtt
     1081 cggattgcag gagagggcca tggcgatcat gacgcgctgc cgcattccgc ccgacatctg
     1141 gtgcgggtac tctttcacgc gacgctcggg atccgggatg cgcacgagtc gcagcatctc
     1201 gatggccttg ttccacgaat catgcttgga gaggtgctgg tgcaggcgaa taccctcggc
     1261 gatctggtcg ccgacggtga agaccgggtt cagcgacgtc atgggctcct ggaagatcat
     1321 ggagatggag gcgccccgga ccttccgcat ctcgttctcg gggaggtcca gaaggttgcg
     1381 cccgtccaac agaatcttcc cggcgacgat cttccccggc gggtcaggga tcaggcgcag
     1441 gatcgagagg gcggtgacgc tcttgccgca gcccgattca cccacgatcc ccagcgtctc
     1501 gcccttgcgg accttgaggc tcaccccgtc taccgctcgg acgacacccg catcggtgaa
     1561 gaaattggtc tgcagcccat tgatctccag caggacgcca ttcttgcttg ccgtggtctg
     1621 atccatggat ggcccttttc gcgaaggctt ccgggaaaac gcgcgtcagt ctagcatcgc
     1681 cttcaggaca gatcaactca aaaaatacct ccgtcggtgt attctccgca acccctccag
     1741 gcctaccgcc tggccgtcac ctgggggtcc agggcgtccc ggaggccgtc gccaaggaag
     1801 ttcagggaga gcaaggtcag cgtcagcgcc aggcccggga agacgatcag ccagggatac
     1861 gtctccatgg cggccgcgcc ctccgaggcc agtgatcccc aactcgccat ggggggctgg
     1921 accccaagcc ccaggaagct caggaaggcc tcctccagca tgaccgccgg caccgtgagg
     1981 gttgcgtaca ccacgatcgg gccgagggcg ttcagcagca agtggcgtgt cacgatgcgc
     2041 gatgccgacg cccccaatgc ctgcgcggag aggacgaact cccgcacttt caggctcatc
     2101 acttgacccc ggacgatgcg tgccatggtg agccattgga ccgccccgag ggcgatgaac
     2161 aggttgagaa tgctgcgacc gaagatgacc atgaggatga tcacgaagaa catgaagggc
     2221 agcccataca tgatgtccac cacccgcatc atcaggtgat ccagtcggcc acctacagag
     2281 ccagccacgg agccatagct caccccgatg gtcagactca ccacgctggc caggattccc
     2341 accgcgaagg agatgcgcgc cccgtagaga atccgagtca agaggtcccg gcccagctca
     2401 tccgtcccca ggaggtgggt cccggacggg ggcttcgccc cgtaacgcag gtctgtccga
     2461 tcgtacggat agggggagat ccagggtgcc gccaagccgc tcaggaccag acaggcgagg
     2521 acgcccaagc ccagcatggc aagcttgttc cgctggagcc tccgcaaggc gtcatgccac
     2581 ggcgacgcgc tggatgcctg ccttccatcc ccgctcccgg ctcggcgctc gcggctcgtc
     2641 attgcacctg cacccgcggg tcgagatagg cgtacagcac gtccacgacc agattgagca
     2701 gcaggagcag gacactgtag aagaccaccg ttcccaagac catggtgtag tcgcgattca
     2761 gggcgctgcc cacgaaatac cgcccgatcc ccgggatgct gaagatggtc tccaccacca
     2821 ccgacccggt gagaatcgcc gccgacgccg ggccgagata ggtaaccacc ggcaggatgg
     2881 cgctgggcag ggcatggcgc aagacgacca ctgcctcacg caggcccttg gcccgagccg
     2941 tgcggacgaa gtcctgcccg atcacctcca gcattccggc ccgggtgagc cgcgcgacgt
     3001 aggccgcata gaaggtcccg agcgtgaggg atggcaggac cagatgacgc caggttcccc
     3061 agcccgccac gggcagccac cccagccaga gggcgaaaac cagcatcagg accggcccca
     3121 ggacgaagtt cggcaccgag acgccgccca gggcgaagga catggtgagg taatccagac
     3181 aggtatttcg ccgcaggccg gcgacaatcc cgctggtgcc acccagcagc acggccacgg
     3241 ccaggccgca cagccccagg ctgagagaca cggggaagcc gaggccgagc agttcgttga
     3301 ccgatcgatc cggatagcgg aaggacgggc ccaggtcacc ccggaggacg tccccgaggt
     3361 agcggagata ctggcgcgac agcggctcgt ccagatggta gcgggcctcg atgttggccc
     3421 gcacctgctc cgggaggact cgctcctgat cgaaaggccc ccccggcgcg aggcgcatca
     3481 tgaagaacgc cagggacacg atcacgaaga ggaccgggac gaaccccgca acccggcgga
     3541 cgatgtagcc cagcacggcc ggatcactgc tccaggctga tgtacttcaa ggggtgttgg
     3601 tccaggatgt tcggatacca gcccttcacc cggcgactga gcatacccgt gttgacgtag
     3661 gtgtagagcg gcaggacggg cgcctcgtcc acgaggatct gctccgcggt ctgcaaggcg
     3721 tgcatccgct ccttggggtt cacgatcttg cacgccgcct gggcgatcag atcatcatat
     3781 cgcttgttcg accagccggt ctcgttgttt ccccctcctg tgacccacat gtccaggaag
     3841 gtgttggggt ccacgtaatc cccgatccag cctgcccgcg cgatctgata ctcgagggtg
     3901 ctctgccggg ccaggtagac cttccactcc acgtttgcga ggtccacctg gatccccagg
     3961 ctgtccttcc acatctgctg cacggcctgg gtgacgacgc gatgcagttc gttcgtgttg
     4021 tacagcagct cgatcttggg caaccccttc ccgtccgggt acccagattg cgccagtagc
     4081 ttcctggccg cggccgcgtc aaagccgagg cctctcgccc cctcataccc gcgcatgcct
     4141 gccggcacga aggtgctcgt cgggatctga cccgccttgg tcacgtactt tgtgagcgcc
     4201 tcgcggtcca cggcgagact tagcgcttta cgcacgcgga tgtcgctcgt gggcggcttc
     4261 gtgacgttga cacggagata gtacgtcccg agatagggcg tgacatagaa ctcgggtcga
     4321 ctccgcgcgg cctcggtctg cgcggtggga acctgaggca cccagtcgct ctccccggcc
     4381 aggaactgct tgtaggcagt gttgacgtct tccgtgggaa ggaacaccac cttctggagc
     4441 ttcacccgcg acgcgtccca gtgctggggg ttcttctcca ggaccagttc ctgctgcggg
     4501 acccagctcg ccagacggaa ggggccgttg gtcacgatct tgcccggctt gacccagtcc
     4561 ttgccgtagg attcgacggc ccagcgtggc accgggtaca gcgtgtagaa gctcgtgagg
     4621 tcgagaaagt aagcggtcgg acacctgagc gtcacctgga gtgtgaggtc gttcagggcc
     4681 ttcaccccga cctgagagaa atcggtgatc cgtcccttgt tgtagtcttc tccgttcttc
     4741 aggtagaaga gctgctgggc gtatttcgcc ccgaacttgg ggttcagggc tcgttcccag
     4801 gcatacacga agtcgtgggc cgtcaccggc ttcccgtcgg tccagcgggc ctcccgcagc
     4861 ctgaaggtat acaccaggcc gtccttggag acggtccagg aggtggccat ccccggcctg
     4921 ggcgacagat ccttcgggtc ggccgtcgtc agtccttcga agaggttgct gaggatccga
     4981 tgctccggta cgcctgtgac caccccggga tccaggctct ccggctccac catgttgttg
     5041 atccggagga cctgcccctc cgcgcctgcg gccggcgcgg caaccgcggc ccccagcagc
     5101 aggatggcca gcatgtgtcc catgggcttc atcacgtctc ttccctcccc gacccctggc
     5161 tgacatgccg cttccaacgc cgcagccctg ggccctctgg cctctgcaac tcagacgcgc
     5221 atacgcgggt cgagggcgtc ccgtagcgca tcccccacga ggttgaatcc gagcaccgac
     5281 agcatgatgg ccgcccccgg aaagaaaaat gcccaccacg ccccgctggt gaaaaacttg
     5341 tagctgtccg ccagcatggc gccccattcc ggcgtcgggg gctgagcccc caggccgagg
     5401 aagccgaggg ccgccgcctc gagaatggca aaggcgactg ccagggtggt ctggaccgcg
     5461 agcggaggca gcgtgttcgg gaagatgtgc cacagcacga tccagtgagg gctgccccca
     5521 agtcctctcg cggcggtgac gaactcacgc tccttcaggg ccaggacctc gccgcgggtg
     5581 atccgggcat aaaatggaat ggagacgacg ctgatggcga tgagcgagtt gcgcaacccg
     5641 gggccgatca tggcgacgat cgcgatggcc aggagcgtcg ccgggaaggc cagaaggatg
     5701 tccatgcaaa acatcaggca cagatccacc cagcggcccg cataccctgc caagagaccc
     5761 agcactgacc cgatgagacc cgcgagcaag accgagctca cccccaaccc gagggaaacg
     5821 cgagcgccat ggaagatgcg catcaggata tcccgcccca gggcatccgt cccgaagggg
     5881 tgggtcccgc tcggaggttt gaggcgggcc aggagatccg agtcaagctg ggggttgtac
     5941 ggaagcaaga cagggctcag gacggccatc aggacgaaga cggcgatgat gacgaggcct
     6001 ccccgggcga catggttggc cagcagccga tgcacggctg cccccagggg gctccggcca
     6061 cgtgtcccgc tgcgggactc gagcggcaat tcaatcgtac ttgatgcggg gatcgactgt
     6121 tgcatacagc acgtccgtga tgagattcac catgacgaag atcgtggcga tgaacagcgt
     6181 cgccccctga acgatggggt aatcgcgcgc ctggatcgat tcatagatcc agaggccgat
     6241 gcccggccag gagaagatcg tctcggtgag gatcgccccg gccaggaggc ggccgacctg
     6301 gagaccggtg acggtcatca ccgggagcag cgcgttccgc atggcgtggc gcaggatcac
     6361 cgcccgctgg gggagcccct tggattgcgc cgtccggatg tagtcctggg agaggacctc
     6421 cagcaagctg gcgcgcgtca tccgggcaat gaccgccatg ggaatggtcg aaagggcgat
     6481 ggccggcagg accaggtggc ggaggctgga gaggagcgcg gccgggtccc gccgcagcag
     6541 gctgtccaga atattgaagt gcgtcaccgc caccagcgtg acgtcggtgc cgagacggaa
     6601 gccggtcggc agccacccca gcaccacccc gaagcaccag gccagcatca gcccgagcca
     6661 gaagatcggc atcgagacgc cggtcagcgc caccagccgc gagaatccgt cgaagaatga
     6721 gtttcgccag accgcggaga cgatccccat cgggacgccc accaggatgg cgatcaggat
     6781 ggcggaggtg gccagctcga ccgtcgccgg gaagcgtcgg accaggtcgc ggagaaccgg
     6841 gtcgcctcgc aagatcgagg cccccagatc cccccgaagg attttgccga cgtagatgaa
     6901 atactgctgg tagaggggtt gatccaggcc gagctgcgcc cgcacctcgg ccacccgctg
     6961 gggcgtcgcc cgctccccca gcatggcgac cgccggatcg ccagggatga gatgggtgaa
     7021 actgaaaacg atgagcgaga ccccgacgag gacggggacg accgtcagaa atcgtgtgat
     7081 ggcatagcgt cccatgtcgt ccccgccctc gtcggcctga acgtccctac ggctgaatgc
     7141 tgaccgtgtt gaagtactcg ctgctggtcg gattggcgac gtaccccttt accttcttca
     7201 ggaacgccag gggcggctgg ttgtgtgcga tgaagatccg ggccgcgtcg tcgtgaatca
     7261 tctgttcagc cttgcggtag agggtagccc gcttccctcg atctgtcagt ttctgggcct
     7321 cgagcaaggt atccgtcagc gcccgattgg cgtagaagcc catgcgcggt tccccgtagg
     7381 cgcagaagaa gtagcatacg aagttgtcgg ggtccccatt gtcgccggtc cacccgagca
     7441 tccagagcgg cacctgtccc ttccggctct tgtcgaggta cacggtccag tccgtggtct
     7501 gaagctgcac actgatcccc accttggcca gatcggcggc gatggcctcg gcgatctcct
     7561 tcgggttcgg atagtacggc cgcgagaccg gcatgtacca gaactggagc tgctccttgc
     7621 ggccgtcgtc ccaggtgata tccttcaggc cctggccgaa cccggcatcc ttcaacaatt
     7681 ctctcgctcg ctgggggttg tactcgtaat ccttcagctc atggttgtgg ccccagaggg
     7741 gcggcggctg gaattgcgtc gcaaccatcc ccgtcccgcc gtacaaggcg tccacgatcg
     7801 ccttcttgtt gatggcctgg gcaaaggcct ggcgtacccg tccgtcctgg aattccttca
     7861 ccttgaagtt gaatgccacg taccccgtgg tgttggtcgg gcgcaggatg agctggaggt
     7921 tcgggtcggc cttgaccacc ttcacgtcgt ccgggttcac cccctccatc ccgtggatct
     7981 ctcccgcctt cagcgcggcc agacgctgag agttgtcctt gatgttccgg acgatcaccc
     8041 gctggacctt gggcgcctcg gccttgttcc agtagttctt gttggcctcc aggacgactt
     8101 cctgtcccac cttccactcc acgaacttga agggccccgt ccccacgggg tgcttgctga
     8161 attccagccc ccacttctgc actgctgtgg gactcaccag gtcgaaggtg aacatggcca
     8221 ggttcgcgag gaatggaccc tgggggctct tgagagtgaa ccgaacggtg gatgggttga
     8281 cggcctccac cttggtgatg aggctcttgt cgtcgaagcc gccgaactgc gccccgtagt
     8341 actcgaacgt cctccccgcc ttggtcaaca tctcgtgctg gggctgtgcg ctgaggcgct
     8401 gccaatcgaa gttccagaca acggccgccg cgtcgaaggg cgtcccatcg tggaacttca
     8461 cgttctttcg gagggtgaag gtccacacgg tgccatccga gctcacttgc catttctcgg
     8521 ccagggccgg gatcacctct gtcgtggctc ccttgtactt cgtcagcccc tcgtagatct
     8581 gccgggtgat ccgggaggag atgccgtcgg tgatgatggc ggggttgaga tccaccggct
     8641 caccctggcc cccaaagatg aaggtggtct gggccaaggc cggcccaccc cagcacacga
     8701 gacccgcgag caggatgaaa cttaagatcc gcagcattcg gtccatggct caaccctcct
     8761 gttgagacga gcagattacc gcagttgtcc cagtgcgctc gccatccgat ccatgccctt
     8821 ctcgatgttc cccatcgacg tcgcgtagga gattcgaaca tgggcgtcgc tgccgaactc
     8881 cacccccggc accacggcga tccgcgcggc ctccaggagg aaggcggaga acttcgtgga
     8941 gttcgtgaca accgtgtccg caaagctctt gccgtacatg ccggagacgt tcgggaagac
     9001 gtagaaggcc ccctcgggat tgagacagcg gacctccggc atggcgttca gccggtccac
     9061 gatgtaccgc cgtcgccgcg caaattcttc ccgcatcctg tgggtcggct cctggggtcc
     9121 cagcagggcc gccacggccg cccgctggga gacggaggtc gggttagagg tcatctggct
     9181 ctgcaacccg tccatggccg cgatgatctc ggcagggccg gccgtgtagc ccacccgcca
     9241 tccagtcatg gcgtacgcct tggatagcga gttgaccagg atcgtgcggg cgtaggcctg
     9301 actccccagg gaggcgatgc tcacatggcg atagccgtcg tacaccaact gctcgtaggt
     9361 ctcgtcggac aggatccaga ccccccgctc tacggccaga gccgccagcc cttccagttc
     9421 cttccgggtg aaggccgccc cggtgggatt ggacggtgag ttcagcacca gcatcttggt
     9481 gtgaggggtc accgcctgct ccagcagttc gcggctcagg cgaaatccct cctgctccga
     9541 agtctgaacg aacaccggcg tggccccgca aagccgcacc atctcggggt agctgaccca
     9601 gtagggggcg gggatgatga cctcgtcgcc gggatccaac atcgcctgga agacattgta
     9661 cagggcgtgc ttgcccccgc aggagatcag gacttggttc cgcgcgtagg tgaggccgtc
     9721 gtcccgcttc aacttgctcg ccaccgcgtc cttcagttcg tcgatgccgc cggttgcggt
     9781 gtacttggtg aagccgtccc ggatggcctg aatcccggcc tccttgatgt ggtcaggggt
     9841 gtcgaagtcg ggttcgcccg cgccaaagct gatcacgtcg atccccccgg cgcgcatgcg
     9901 ccgggcggtg gcgctgatgg ccagggtggc agaagggacg agattcaggg ctcgctggga
     9961 gagcgtcacg tggtcctcct tcccagatga gggagctggc ttgcgcgctc cttcaggagc
    10021 ttggccgcca ggggcgggac gaggtcgtcc acgttccctc ccagaagcgc aacctctttc
    10081 accagccggg agctgagata ggtgtaggcc tggttcggca tgaggaagac ggtctcgacc
    10141 tgatggctca tcttccggtt catgagggcc atggcgaact cgaactcata gtccgagacc
    10201 gcccgcacgc cccgcaggat gacctgtgct cccttgctca acacgtagtc caccgtcagg
    10261 gcctggaagc tgtccacctc cactccgggc atgcccgccg tggcctgccg cacgatggcg
    10321 atccgctcct ccaaggggaa caggggattc ttgtgcggat tgccggccac cgccacgatc
    10381 aggtgcgtga agatccggcg cgcccgctcc accaggtcga tatgaccgtt ggtgaaggga
    10441 tcgaaggtgc cgggataaac cgcggtcact tgcatggcga ctccctgacg tagaacgtga
    10501 gtgcgctttc tccgtaccgt cgctgatcgg accgccgcag gacgccggcc tgccccggca
    10561 gggcggtctt gtggaatgcc tggaccacga gcagcgcatt ctcgctcaaa atgcctccgg
    10621 gggcaagtgt ttcgatgcac ccgaccgcct ggtccgcggc gtagggcgga tccaggaaga
    10681 cgcagtcgaa ggtctctccc tcctcctgca actcccccag gatgggcagg acctcgccca
    10741 gcaagatccg agaccgcgcc ccgaggtcca aggctgtcag attcgcccgc agactggcca
    10801 ctgcccgccg atcccgctcc acgaaggtcg cgccggccgc gccgcggctg agagcctcca
    10861 gcccgatggc ccccgtcccg gcgtacagat cgagaaccac cgccccccgg atgcgctcgc
    10921 cgatcatgtt gaagatcgcc tgccgcagga actccgatgt cggccgggtc cgaaggccct
    10981 tggggctctg aaggtgacgt ccgcggtgca gtcctgcact gattctcatc gtggtagaga
    11041 cgccagattg aacgaccgcc catcagccga cctgggccaa ccccaggcgg cttttccagc
    11101 gctgctccat ggccgcgcgg accgccgacc actccccgtc cagttcgggt gaatgcgcca
    11161 ggaccgccat cgcctcggcc cgggcatcct ccagcagccg gagatccgcg cccagatccc
    11221 ccagttgaaa cccgtcgaga cccgcctggc gcgttccgaa gaactcaccc gggccacgca
    11281 gccgaagatc ggcctccgcc agggcgaagc cgtcaccctc tcgcacgaac acctccagcc
    11341 tcgcccgggc ctcctccgag atttcatgcc ggtcggcaac cacgaaacag accgcggggc
    11401 aactcgaccg gccgacgcgt ccgcgaagct gatggagctg cgctaccccc agacgatcgg
    11461 cgtgctcgat caccatggcc gtggcctcgg gcacgtccat ccccaccaca accacggtgg
    11521 tggccaccag gagccggacc cgcccctcgc ggaagtctgc gaccgcctgc agtttctcac
    11581 cggcgcggag ccgtccgtgc accagcccca cgccgaatcg ggccaggggc ccgagccggt
    11641 acgcttcggc cgtctggagc gccgccttca gctccgccgc ggactcctcc accacggggc
    11701 agacaacgta g
//
LOCUS       DBNGZC010000944         4047 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1943498, whole genome shotgun sequence.
ACCESSION   DBNGZC010000944 DBNGZC010000000
VERSION     DBNGZC010000944.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1943498"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..168
                     /locus_tag="AC16G0_16330"
     CDS             <1..168
                     /locus_tag="AC16G0_16330"
                     /inference="COORDINATES: protein motif:HMM:NF025923.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylpyrophosphate synthetase"
                     /protein_id="HIR5101223.1"
                     /translation="ERLEHSAIEEVVVTNTIPLREDQQCKKVTVLSVATLLGEAIRRI
                     HTETSVSSLFV"
     gene            253..960
                     /locus_tag="AC16G0_16335"
     CDS             253..960
                     /locus_tag="AC16G0_16335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HIR5101224.1"
                     /translation="MATVDLKSEVRTTLGKEEAKRMRRLLRIPGIVYGGSHGTIPVVV
                     NPNELLSVLGAGENVLINLSLRKGDGAEQFTVILKELQIDPVKGRPLHADFMEISMER
                     KIRVEVPLVLTGESVGVKGKGGILEQPLRQLFVECLPLNIPEKIVVDVSLLDIGDAIH
                     VRDLPVAEGIRTLEDGDRVVASVVAPAAEEVAPVVEEEKPAEPEVVGKKEKETPEVEG
                     EAKPKAEAKPKPEAKPK"
     gene            961..1572
                     /gene="pth"
                     /locus_tag="AC16G0_16340"
     CDS             961..1572
                     /gene="pth"
                     /locus_tag="AC16G0_16340"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR5101225.1"
                     /translation="MEAIREAHWLVAGLGNPGAAYAGTRHNVGFLVVERLAAEARARF
                     GPEAFQAEAAWANVSGAAVLLLKPQAFMNLSGPPIGRWLSKLALPVDRLVVVHDDLDL
                     PLGRLRLTGGAGSGGHRGVASIQAVLGTRDFPRVRVGIGRPDEDEDAAARVLGEFRPE
                     EYPLVQETVARAVGAVLSLIGDGLTAAMSRYNVRVMPDASASA"
     gene            1610..1960
                     /gene="rpsF"
                     /locus_tag="AC16G0_16345"
     CDS             1610..1960
                     /gene="rpsF"
                     /locus_tag="AC16G0_16345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR5101226.1"
                     /translation="MRSYEVIFILDPALAEDAVEAAILAAKGVVTKEGGEVAEVQKWG
                     KKRLAYEIKKRREGHYVFFRISAPAKAVVELERHLKIAETVLKYLTVLMQTSGRKFGK
                     PKTPVAVDAAVQEA"
     gene            1963..2373
                     /locus_tag="AC16G0_16350"
     CDS             1963..2373
                     /locus_tag="AC16G0_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005009857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR5101227.1"
                     /translation="MASFNKVILMGNLTRDPELRYTPSGAPVCNFDLAVNRSYTTQGG
                     ERRDEVCYVTIVVWGKQAESCGEYLGKGRTVLVEGHLQQRSWETPEGQKRSKHEVVAE
                     RVQFLGPRKGAAAAAPGEEEPEPVRTPEQDEVPF"
     gene            2462..2698
                     /gene="rpsR"
                     /locus_tag="AC16G0_16355"
     CDS             2462..2698
                     /gene="rpsR"
                     /locus_tag="AC16G0_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR5101228.1"
                     /translation="MLKKRRTYRRQKVCKFCFDKVVQVDFKDVKRLRNFVSDRGKIIP
                     RRISGNCAYHQRQLTVAIKRARSIALLSFAAEMA"
     gene            2734..3693
                     /locus_tag="AC16G0_16360"
     CDS             2734..3693
                     /locus_tag="AC16G0_16360"
                     /inference="COORDINATES: protein motif:HMM:NF021506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HIR5101229.1"
                     /translation="MSQPRWVGQAAESILAVLLAVGLFASGGLLPVVGVTLSLLCPLP
                     LVLLRLRHGPLALGVALTLATLALAGPLSWWQALAFLLEFGLPAVLLAEGLRRGHRPE
                     LVVTGVAAVLTLGGVGVLVLSSGEWTHPLAAVARHVETLVTDMEALTARIASPGEGTS
                     PLAMPIPNLRAFLLMAFPGLFFAGSLLTAAGYVLLLRGLTRRWPAQLGGISAGPFKWE
                     LPEALVWGFIGSGALYLTGHPWLTAVGLNGLILLVALYFLQGLSIAAFLYQRFKLPRF
                     LVTLSVLLLVFQPFLALVVAGLGLFDVWFAFRRLHLPKTPRGT"
     gene            3775..>4047
                     /gene="rplI"
                     /locus_tag="AC16G0_16365"
     CDS             3775..>4047
                     /gene="rplI"
                     /locus_tag="AC16G0_16365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR5101230.1"
                     /translation="MKVILMQKVERLGEPGQQVDVADGYARKFLLPKQLAVLATPGRV
                     RSLTQLTAQAKNREDRSKREATSLADRLAPVQITLKRRASEDTPLVL"
BASE COUNT          663 a         1187 c         1513 g          684 t
ORIGIN      
        1 gagcggctcg agcactcggc catcgaggag gtcgtggtga caaacaccat ccccctgcgg
       61 gaggatcagc agtgcaagaa ggtcaccgtg ctgtcggtgg ccacgctcct gggggaggcc
      121 attcgccgta tccacacgga gacctccgtc agctcgctgt ttgtctgaat gcggcgggca
      181 cgggcggcgc ggcgggcggg atgaggcacg taacacgctc ctgaatccta caacgggcag
      241 tcggaggcga gcatggcaac ggtggacctg aagagtgaag tccggaccac gctggggaag
      301 gaggaggcga agcgcatgcg ccggctgctt cgcattccgg ggatcgtcta tggcggatct
      361 cacgggacca ttcccgtggt cgtgaacccc aacgaactgc tctcggtgct gggtgccgga
      421 gagaacgtcc tgatcaacct ctcgctgcgg aagggggatg gggcggagca gttcacggtg
      481 atcctcaagg agttgcagat cgatccggtc aaggggcggc cgctccacgc cgacttcatg
      541 gagatctcca tggagcggaa gatccgggtg gaggttcccc tggtcctgac cggcgagtcg
      601 gtcggagtga aggggaaagg cggcatcctg gagcagccgc ttcgccagct cttcgtcgag
      661 tgccttccgc tcaacatccc ggagaagatc gtggtcgacg tctccctact ggacatcggt
      721 gacgccatcc acgtgcggga tctgccggtg gccgagggga tccggaccct ggaggacggc
      781 gatcgggtgg tcgcctcggt cgtggccccg gccgcggaag aggtggcgcc ggtggtcgag
      841 gaggaaaagc cggccgaacc cgaggtggtg ggaaagaagg agaaggagac gccggaagtc
      901 gagggggagg ccaaacccaa ggccgaggcc aaacccaagc cggaggcgaa gcccaagtag
      961 gtggaggcca ttcgcgaggc gcactggctg gtggcggggc tggggaatcc cggcgccgca
     1021 tacgcgggga cgcgacacaa cgtgggcttc ctggtggtgg agcgtctggc ggcggaggcc
     1081 agggcgcggt tcggccccga ggccttccag gctgaagcgg cctgggcgaa tgtgtctggg
     1141 gcggcggtcc tgctgctcaa gccacaggcc ttcatgaatc tgagcgggcc gccgatcggg
     1201 cggtggctct ccaagctggc attgcctgta gacaggctgg tggtcgttca cgatgacctg
     1261 gatcttccgc tgggcaggct gcgcctgacc gggggggccg ggtcgggcgg tcaccgcgga
     1321 gtggcgtcca tccaggcagt gctggggacc agagatttcc cgcgggtgcg ggtggggatc
     1381 ggccgcccgg acgaagacga ggacgcggcc gcgcgggttc tgggcgaatt ccggccggag
     1441 gagtaccccc tggtgcagga gacggtggcg cgggccgtgg gggccgtgct gagtctgatc
     1501 ggcgatgggc tgacggccgc catgagtcgc tacaatgtgc gggtaatgcc ggatgcgagc
     1561 gcatccgcct gaggcatgga aggcatagtc gggagaggag gtgaacggat tgcgttccta
     1621 cgaagtcata ttcatcctgg atcccgccct ggcggaggat gcggtggaag ccgccatcct
     1681 ggccgccaag ggggtggtga ccaaggaggg gggcgaggtc gccgaggttc agaaatgggg
     1741 caagaagcgc ctcgcctatg agatcaagaa gcgacgcgaa gggcattacg tctttttccg
     1801 gatcagtgcg ccggcgaaag ccgtggtcga gctggagcga catctgaaga ttgcggagac
     1861 ggtcctcaag tacttgacag tgctcatgca gacttcgggc cgcaaatttg gcaagcccaa
     1921 gacacccgtg gccgtcgatg cggcggtcca ggaggcctga gcatggcgag cttcaacaag
     1981 gtcatcctca tggggaacct cacgcgtgac cccgagctgc gctacactcc cagcggggcc
     2041 ccggtgtgca acttcgacct ggcagtcaac cggtcgtata ccacccaggg aggggagcgg
     2101 cgggacgagg tgtgctacgt caccatcgtt gtctggggga agcaggcgga gagctgcggg
     2161 gagtacttgg gcaaggggcg tactgttctc gtcgaggggc atctccagca gcgctcctgg
     2221 gagacccccg agggacagaa gcgttcgaag cacgaggtcg tggcggagcg ggtgcagttc
     2281 ctgggcccgc gcaagggagc ggcggccgcc gccccaggcg aggaggagcc ggaacccgtc
     2341 cggacccccg agcaggatga ggtcccgttc tgaacccgga cggtatgacg ggtgcggatg
     2401 ggacccacga ggatgctgag acatcgcccc ggatgccccg cgggcgagag gagtgaggcg
     2461 cgtgctgaag aagcggcgaa cctaccggcg tcagaaggtc tgcaagttct gtttcgacaa
     2521 ggtggtgcag gtggatttca aggacgtgaa gcgcttgcgc aatttcgtca gcgaccgggg
     2581 gaagatcatt ccgcgacgga tctcgggcaa ttgcgcctac caccagcggc aactcacggt
     2641 ggcgatcaag cgggcgcgga gcatcgcgct cctgtccttt gcggcggaga tggcctaatc
     2701 tcccgagcgg ggtggcgcga gcggaggatg cgggtgtctc aaccacgatg ggtgggtcag
     2761 gccgcggaga gtatcctggc cgttctcctg gcggtcggct tgttcgcgtc gggtgggctg
     2821 ctcccggtcg tgggagtgac cctcagcctg ctctgcccgc tccccttggt gctgcttcgg
     2881 ctgcgccacg gacccttggc cctgggcgtg gccctgacgc tcgcgaccct ggcgttggcg
     2941 ggtccgctct cgtggtggca ggccctggcg tttctcctgg agttcggatt gccggccgtc
     3001 ctcttggcgg aaggcctgcg gcggggacac cggccggaac tggtggtgac gggtgtggcg
     3061 gccgtgctga ccctgggcgg ggtcggtgtg ctggtgttga gctcgggtga gtggacccat
     3121 cccctagctg ccgtggcgcg acacgtcgag accctggtga cggacatgga ggccctgacc
     3181 gcccggatcg cctctcccgg ggagggcacg tcgcccttgg ccatgcccat cccgaatctg
     3241 cgcgccttct tgttgatggc tttcccgggt ctcttctttg cgggcagtct gctcacggcg
     3301 gcgggctacg tgctgctctt gcggggcctg acccgacggt ggccggcgca gctcgggggg
     3361 atttctgccg ggccgttcaa gtgggagctt cccgaggcgc tggtgtgggg gttcatcgga
     3421 tcgggggccc tgtatctgac aggtcacccg tggctcacgg ccgtcgggtt gaacggcttg
     3481 attctcttgg tggccctgta cttcctccag ggtttgagca tcgcggcgtt tctgtaccag
     3541 cggttcaagc ttccccgctt cctggtcacg ctgtcggtgc tcctcttggt gttccagccg
     3601 ttcctggcgc tggtggtggc aggactcggg ctctttgacg tctggttcgc cttccggcgg
     3661 ctccacctcc ccaagacccc ccgcgggacc tagacgaaga cggggcccct ggtcagggga
     3721 gcggagggac ggaaaaccga gggcggatcg cacctcaatc aacggagcgc gctcatgaaa
     3781 gtgatcctga tgcagaaggt cgaacgactg ggagagcccg ggcagcaggt ggacgtcgct
     3841 gacggctacg cccgcaagtt cctcctgccc aagcagttgg ccgtcttggc cacgccgggc
     3901 cgggtgcgat cgctgacaca gctaaccgcc caggccaaga accgcgagga tcgatccaag
     3961 cgcgaggcga cctcgctggc ggatcggttg gccccggtgc agatcacgct gaagcggcgc
     4021 gcgagcgaag acacgcccct ggtcctg
//
LOCUS       DBNGZC010000945         1505 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_666489, whole genome shotgun sequence.
ACCESSION   DBNGZC010000945 DBNGZC010000000
VERSION     DBNGZC010000945.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_666489"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            542..1120
                     /locus_tag="AC16G0_16370"
     CDS             542..1120
                     /locus_tag="AC16G0_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101231.1"
                     /translation="MAEPQLATIVKDWVTAAGIVIGGTWALWKWGFSEFLRRLREIPS
                     LDGRMTAVPVPITSKEVLVTFEALWRNRGQLPVPLDPARTGFKVYELDRAFAIGPLEP
                     QLGATPAHHTALPFAGLSGFLLEPATESVLQAHFVLRRGVPYLVRSELVAPGGNASWQ
                     RDLLWSIEATASSDEATTASWLRRLVERSRTR"
     gene            1237..>1505
                     /locus_tag="AC16G0_16375"
     CDS             1237..>1505
                     /locus_tag="AC16G0_16375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101232.1"
                     /translation="MPDLAEPFETWFLQRVARAIEAGDVPADLLTGLRTEMERGRELA
                     QEEGHALAVQDLADQLGLPVDQAEMMLCALESQPTVTRELLLRRIV"
BASE COUNT          254 a          482 c          490 g          279 t
ORIGIN      
        1 ggccgactac ttcttctaca agaaggagcc cggcaactag gatctctgca caagtgcggt
       61 gggagaaccg gacggcccaa ccgtccggtt ctccgtatca gtcgctcagc ttctcaaggt
      121 agaggccagg tggaggggca ggcttgaatc gttgaaaagc tatgtgcctc ttaggcgcac
      181 catcggtttc attccaggca gcttggccgc cctgcacgtt cgagcggacc ccggcatccc
      241 gttgctcgcc cttggccgct gatgttcgtc gttcatcgca atcttccgcc tcagaggacc
      301 agacgcccca tgacctccca ccgcctcttc accctcagcc ccgcccacga gccgcgctgg
      361 atccgcctct acgtccgccg gattggcgag aagtgggcgg cggcacgtgt ggccgacgga
      421 gtgctaccgt cggagccggt tcacctgggg gcagactgga gttgcaaagg gccgcaacca
      481 agcgcagtga aagacgataa cgcggggatc gcgcctgctt tcgcaggagg ggggaaggac
      541 catggcggag ccgcagctcg ctactatcgt caaagattgg gtgaccgccg ctgggatcgt
      601 cattggcggg acgtgggcac tgtggaagtg gggcttctcg gagttcctcc gtcgcctccg
      661 ggagatcccg tcgctggatg ggcggatgac ggcggtcccg gtgccgatca cctcgaaaga
      721 ggtgctggtc accttcgagg ccctctggag aaaccgaggc cagctgcctg tcccgcttga
      781 tcctgcaagg acgggcttca aggtgtacga gttggatcgt gccttcgcca tcggcccgtt
      841 ggagccgcag ctcggggcca ccccagccca ccacacggcc ctcccctttg caggactctc
      901 ggggttcctt ctggagcccg cgaccgagag cgtcctccag gcgcacttcg tcctgcgccg
      961 aggcgtgccg tacttggtgc ggtcggaact cgtcgcgccg ggtgggaacg cgagctggca
     1021 gcgtgaccta ctgtggtcca ttgaggcgac ggcctcctcc gatgaggcga caaccgcatc
     1081 ttggctccgc cgtctagtgg agcggtcacg tacccggtga tctctaaggt cccacgtcct
     1141 accggagtag ccgcgactca tgactgatca ggggtgaggt agcccaagct gtcgcgttat
     1201 gctggtattc ccgcaatcca gccttgacag cgtcctatgc ccgatctagc ggaacccttc
     1261 gagacctggt tcttgcagcg cgtcgctcgg gccatcgagg ccggagacgt gccggcggat
     1321 ctcctgactg gactccgcac ggaaatggag cggggcaggg agctagccca agaggaaggc
     1381 cacgccttgg ctgtccagga cctcgccgac cagttgggcc tcccagttga tcaggcggag
     1441 atgatgctgt gcgccctcga atcgcagccg accgtgacgc gggaactgct gctgcgtcgg
     1501 atcgt
//
LOCUS       DBNGZC010000946         3053 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2333708, whole genome shotgun sequence.
ACCESSION   DBNGZC010000946 DBNGZC010000000
VERSION     DBNGZC010000946.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2333708"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="AC16G0_16380"
     CDS             <1..202
                     /locus_tag="AC16G0_16380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5101233.1"
                     /translation="EVREAHVFGDALHLIVTDAEGGLARVEAVLRAQGLQVGQLRPIT
                     PGLEDVFVTLLRDQTTRPSAGR"
     gene            299..1237
                     /locus_tag="AC16G0_16385"
     CDS             299..1237
                     /locus_tag="AC16G0_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5101234.1"
                     /translation="MDDDLAIQVLDLTKRFGHFLAVDRVSFQVRRGEVFGFLGPNGSG
                     KSTTIRMLCGILMPSSGTARVAGFDVARDPERVRENIGYMSQKFSLYEELTVQENLDF
                     FAGIYGLAGSEAALRREEVLALGGLAASRNVLAHDLPGGLRQRLALASAIIHRPSVLF
                     LDEPTAGVDPISRREFWELIRQMAAAGTSVLVTTHYMDEAEHCHTLALIYQGRLIAMG
                     APSHLKTGLHGSLVEVEVAPAELVPALRALQDNPAAHEVAMFGAALHVTLDSSEALPA
                     LQACLQQKGIVPQRMEPILPSLEDVFVSLVAQEVER"
     gene            1234..2370
                     /locus_tag="AC16G0_16390"
     CDS             1234..2370
                     /locus_tag="AC16G0_16390"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5101235.1"
                     /translation="MSWRVWAIMRKEFIEVWRDTRSLIFVLAMPVLMLMLYGYGISSD
                     VKRVPMVVYDRNGRPAARELVSRLTSTDYFVVTAQVDNLQALRRAIDVGQARVGLVIP
                     EDFSQNLGAGTPAPVQFVVDGSDSNTASIAIGNIASIARGTELSPRDLRPHAFVTPAP
                     TTTAPIELRAWIWYNPELKSSNFLVPGLTAVILMMLAAMLTSLTVAREWERGTMESLI
                     ASPLHPHELMVGKILPYVVLGLVDVVFILLVAVFWFHVPMRGSLTLLMVSSTVFLLGG
                     LGIGLFISAATKSQQVAFQLSTLATMLPALLLSGFFYPIENMPPVLQAITTLVPARYF
                     LVVIRGIFLKGVGLAVLWKELLFLGLFALLMLWASSAKFHKRLE"
     gene            2373..>3053
                     /locus_tag="AC16G0_16395"
     CDS             2373..>3053
                     /locus_tag="AC16G0_16395"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5101236.1"
                     /translation="MERSPRSAHPRRLVTLIKKEFIQIRRDRRMLPILVILPVMQLLI
                     LGYAVSSDIKHLATAVCDLDRTAESRAFADKFATSGYFDIRYRIADERRIKELVDEGK
                     VRVGIVIPPRFAKSLASQQTATVQLLLDGTDSNTANVAQGYVTQIVNAESARILVERV
                     GPKAQPPIALDASIQVWFNPDLKSVNFMVPGVIALILTIVTMTMTATSIVKEKERGTI
                     EQLVVSPIR"
BASE COUNT          481 a         1047 c          941 g          584 t
ORIGIN      
        1 cgaggtgcgg gaggctcacg tgtttggcga tgccctccac ctgatcgtca ccgacgccga
       61 gggcggcctt gctcgcgtcg aggcggtctt gcgggcgcaa ggcctccagg tggggcaact
      121 ccgtcccatc acgccgggac tggaggacgt cttcgtcacg ctgctgcggg atcagacgac
      181 caggccctct gcggggcgtt gagcaccatc agcgtccatc cggcttcagt tgaatgctct
      241 tttccctccc ccactacggg ggaattcttg tggcgccgtt atctttcgag gatttcgcgt
      301 ggatgacgat cttgctatcc aggtcctgga cctcacgaag cgcttcggcc atttcctggc
      361 cgtggaccgg gtgagcttcc aggttcgtcg cggcgaggtc ttcggctttc ttggccccaa
      421 cggctccggg aagtccacga cgatccggat gctgtgcggc atcctgatgc ccagttcggg
      481 gacggcccgg gtcgcgggct tcgacgtggc gcgagacccc gagcgcgtgc gcgagaacat
      541 cggctacatg tcccagaagt tctccctgta tgaggagctg acggtacagg agaacctgga
      601 tttcttcgcc gggatatatg gtctagccgg atccgaggcc gccctgaggc gagaagaggt
      661 cctggccctc ggaggtctcg ccgcttcccg gaacgtcctg gcccatgacc tgcccggcgg
      721 cctgcgccag cgtctggccc tggcctcggc catcatccac cgcccctccg tcctgttcct
      781 ggatgagccc acggcaggcg tggatccgat ctcgcggcgc gagttctggg aattgatccg
      841 gcagatggcc gcagccggca cctctgttct cgtcacaacg cattacatgg acgaggcgga
      901 acactgtcat accctggccc tcatctacca ggggcgattg atcgccatgg gggcccctag
      961 ccaccttaag actggactcc acggcagtct cgtggaggtg gaggtggccc cggccgagct
     1021 cgtccccgcc ctccgggccc tccaggataa tccggccgcc cacgaggtcg ccatgttcgg
     1081 agccgccctg cacgtcaccc tggattccag cgaggccctc cccgccttgc aggcgtgcct
     1141 gcagcaaaag gggatcgtcc cccagcggat ggaacccatc cttccctcgc tggaggacgt
     1201 cttcgtatcc ctcgtggccc aggaggtcga gaggtgagct ggcgcgtctg ggccatcatg
     1261 cgcaaggagt tcatcgaggt ctggcgcgac acgcgctccc tgatcttcgt cctggccatg
     1321 cccgttttga tgctcatgct ctacgggtac gggatcagct cggacgtgaa gcgcgtgccc
     1381 atggtggtgt acgatcgcaa cggacggcca gccgctcgcg agctggtcag ccgcctcacc
     1441 agcaccgatt actttgtggt gacggcgcag gtggacaacc tccaggcctt gcgacgcgcc
     1501 atcgacgtcg ggcaggcccg agtgggtctg gtgatcccgg aagatttcag ccagaatctg
     1561 ggcgcgggca cccccgcccc cgtgcagttc gtggtggacg gctccgacag caacacggcc
     1621 agcatcgcca tcggcaacat cgcctccatc gcccggggca ccgagctgtc cccccgcgac
     1681 ctgcgtcccc acgccttcgt gacgcccgcg ccgaccacga cggcccccat cgagctgcgc
     1741 gcctggatct ggtacaaccc ggagctgaag agctccaact tcctggtgcc gggactcacc
     1801 gcggtgatct tgatgatgct ggccgccatg ctcacctccc tgacggtggc ccgggaatgg
     1861 gagcggggaa ccatggagag cctcatcgcc tcccccctcc acccccatga actaatggtg
     1921 ggcaagattc tgccgtacgt ggtcctgggt ctggtggacg tggtgttcat cctgctcgtg
     1981 gccgtcttct ggttccacgt gcccatgcgc ggaagcctca cgctcctcat ggtctcctcg
     2041 accgtcttcc tcctgggtgg cctggggatc ggcctgttca tctcggcggc caccaagagc
     2101 cagcaggtgg ccttccagct ctccaccctg gccaccatgc tcccggccct cttgctgtcc
     2161 gggttcttct atcccatcga gaacatgccc cctgtactcc aggccatcac cactctggtt
     2221 ccggcccgct acttcctggt ggtcatccgg gggatcttcc tgaagggtgt gggcctggcg
     2281 gttctctgga aggagctgct cttcctcggc ctgttcgccc tgttgatgct gtgggccagc
     2341 tcggccaagt tccacaagcg gctggagtga gcatggagag aagcccgcga tccgcccatc
     2401 cgcggcgtct tgtgacgctg atcaagaagg agttcataca gatccgccgt gaccggcgga
     2461 tgctgcccat cctcgtcatc ttgccggtga tgcagctcct cattctgggc tatgccgtct
     2521 cctcggacat caagcacctg gccaccgccg tttgtgacct ggaccggacc gccgagagca
     2581 gggccttcgc cgacaagttc gccacctcgg gctactttga tatccggtat cgcatcgcgg
     2641 acgagcggcg gatcaaggag ctggtggacg agggcaaggt gcgggtcggg atcgtgatcc
     2701 cgccgcgctt cgccaagagt ctggccagcc agcagaccgc caccgtccag cttctgctcg
     2761 acgggaccga cagcaacacc gccaacgtgg cacagggata cgtcacccag atcgtgaatg
     2821 ccgagtccgc caggatcctg gtggagcgcg tggggccgaa ggcccagccg cccattgccc
     2881 tggacgcctc catccaggtc tggttcaacc cggacctgaa gagcgtcaac ttcatggtgc
     2941 ccggcgtgat cgccctgatt ctcaccatcg tgaccatgac catgaccgcc acctccatcg
     3001 tcaaggagaa ggagcggggc accatcgagc aactggtcgt cagccccatc cgc
//
LOCUS       DBNGZC010000947         2328 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1351667, whole genome shotgun sequence.
ACCESSION   DBNGZC010000947 DBNGZC010000000
VERSION     DBNGZC010000947.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1351667"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2328)
                     /locus_tag="AC16G0_16400"
     CDS             complement(<1..>2328)
                     /locus_tag="AC16G0_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009110509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5101237.1"
                     /translation="PAVEGQRLNASIIVQSMLKTPEEFVNIPVRINPDGSIIRIKDVG
                     RAELGTDTYDIEAFHNGRPAAGMAIRQAAGANALETADRVKAKMAEMSRYFPPGMKVV
                     YPYDTTPFIKVAIGEVVKTLFEAILLVFLVMWLFMGNFRATLIPTIAVPVVLLGTFAV
                     LSFFGFSINMLTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLSPKEATRKSMEQITS
                     ALIGIGVVLAAVFGPMAFFGGSTGVIYRQFSVTIISSMLLSVVVALILTPVLCASLLK
                     PVPAGHEPAENAIWFLRPFFAWFDRVFFRLRDRYVKVVGRSFSRKLRYAVVYVVIVVA
                     VGLLFLHSPSSYVPDEDQGILISMVMLPTGATLEQTKQVVSDVQHHFQEHEAAAVESA
                     MMISGIGFSGRAQTNGLVFIRLKDWHLRNRSDLRAKAIAERATRAFSNIRNALVFAFP
                     PPSVIELGTATGFDFQLLDRGGLGHQKLLAARNELLGMAARDRRLTKVRPNGMEDVPE
                     YRVDVDWERAGALGVPISSIHNTISAAFGSRYVNNFIQGGRVKRVFLQADAPYRMLPK
                     DIQKLYVRNTWGKMVPFSSFASAHWTSGAPRLERFNGFPSMNIQGEPAPGRSSGEAMQ
                     AMEEIVAKLPQGIGYEWTGLSYQERMGGSQAPLLYALSVIVIFLCVAALYESWPIPIA
                     NLLMLPLGLFGAALATWLRGMHNDVYFQIGFLTTLGLTTKNAILIIQFAQHRMAHGEG
                     LIEATLGAVRVRFRPVIMTSMAFFFGVLPLAIATGA"
BASE COUNT          467 a          720 c          735 g          406 t
ORIGIN      
        1 cgccccggtc gcgatggcca gcggcagaac cccaaagaaa aacgccatcg atgtcatgat
       61 gaccggccgg aatctcaccc gaaccgcccc cagcgtcgcc tcgatcagcc cttctccgtg
      121 ggccatccgg tgctgggcaa actggatgat caggatggcg ttcttggtcg tcaggccgag
      181 cgtggtgagg aagccgatct ggaagtagac atcgttgtgc atgccccgaa gccaggtggc
      241 cagcgctgcg ccgaatagtc cgagcggcag catcaacagg ttggcgatgg ggatcggcca
      301 gctctcatac agggccgcca cgcacaagaa gatgacgatc acggaaaggg cgtacagcag
      361 gggcgcctgc gagcctccca tgcgctcctg gtaggagagc ccggtccatt cgtagccgat
      421 cccttggggc aatttcgcga caatctcctc cattgcctgc atcgcttcac cggaacttct
      481 tcctggtgcc ggctctccct ggatgttcat ggatgggaag ccgttgaagc gctccagcct
      541 gggagcgccg gacgtccagt gggcggaggc aaaggaggaa aaggggacca tcttccccca
      601 cgtattccgg acataaagct tctggatatc tttgggcaac atgcggtagg gcgcgtccgc
      661 ctgcaggaaa actcgcttga cccgcccgcc ctgaatgaag ttgttgacat acctgctgcc
      721 gaaggccgcc gagatggtgt tatggatgga gctgatgggt accccgaggg cgccggccct
      781 ttcccagtcc acgtctactc gatattctgg cacgtcctcc atgccgttgg gtcggacctt
      841 ggtcaggcgc ctatctctgg ccgccatgcc gagaagctca ttgcgggccg ccaacagctt
      901 ctggtggccg aggccgcccc ggtccaggag ctggaagtcg aaccctgtgg cggtccccag
      961 ctcgatcacc gacgggggtg ggaaggcgaa caccagggca ttgcgaatgt tcgagaaggc
     1021 ccgggtagcc cgttccgcaa tggccttcgc cctcaggtcc gaccgattcc ggaggtgcca
     1081 atccttgagc ctgatgaaga ccaggccgtt ggtctgggcc cgtccggaga agccgatccc
     1141 ggatatcatc atggcggatt ccaccgccgc agcctcgtgc tcctggaaat ggtgctggac
     1201 gtcggacacg acctgcttgg tttgctccag ggtagcgccg gtgggcagca tgaccatgga
     1261 gatcaggatc ccctgatcct catccggcac ataggacgag ggcgaatgca ggaagaggag
     1321 ccctaccgcc accacgatca cgacatagac gactgcgtac cgcagcttcc tggagaagga
     1381 gcgtccgacg accttgacgt accgatcgcg gagccggaag aagacgcgat cgaaccaggc
     1441 aaagaacggg cggaggaacc agatggcatt ctccgcgggc tcatgcccgg caggcaccgg
     1501 cttgaggagc gaggcgcaga ggaccggcgt caggatcaat gccaccacca ccgagaggag
     1561 catggaggag atgatggtca cggagaactg gcgatagatc acgccggtcg aaccgccgaa
     1621 gaaggccatc gggccgaaca ccgcggcaag caccactccg ataccgatca gggcgctggt
     1681 gatctgctcc atggatttcc gcgtggcttc ctttggcgaa agcccttcct cgctcatgat
     1741 ccgttccacg ttttccacca ccacgatggc gtcgtccacc agaaggccga tggccagcac
     1801 catggcgaac atggtcagca tgttgatgga gaagccgaag aacgagagga ccgcgaaggt
     1861 tcccagcagc accaccggca ccgcgatggt cgggatcagg gtggcccgga agttccccat
     1921 gaacagccac atgaccagga acaccagaag gatcgcctcg aagagggtct tcaccacctc
     1981 gccgatggcc actttgatga agggggtggt gtcgtagggg tagacgacct tcatcccggg
     2041 gggaaagtag cggctcatct ccgccatctt ggccttgacc ctgtcggccg tttccagggc
     2101 gttcgccccc gccgcctgcc ggatggccat gccagccgct ggccggccgt tgtggaaggc
     2161 ctcgatatcg taagtgtcgg ttcccagctc tgcacgcccc acatccttga tccggatgat
     2221 ggagccgtcc ggattgatgc ggacgggtat gttgacgaac tcttcggggg tcttcagcat
     2281 gctctgcacg atgatcgaag cgttcaggcg ctgcccctcc acggccgg
//
LOCUS       DBNGZC010000948         2344 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_471949, whole genome shotgun sequence.
ACCESSION   DBNGZC010000948 DBNGZC010000000
VERSION     DBNGZC010000948.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_471949"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="AC16G0_16405"
     CDS             complement(<1..186)
                     /locus_tag="AC16G0_16405"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HIR5101238.1"
                     /translation="MRDERGVIFDLDGVLINSEPLHCQAFQEVLGSYGVTVTDRDYYT
                     NYIVYSDREVLERLLPDR"
     gene            complement(251..1630)
                     /locus_tag="AC16G0_16410"
     CDS             complement(251..1630)
                     /locus_tag="AC16G0_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR5101239.1"
                     /translation="MSHEEILVVDDEKNIRGSLEGILRDEGYRVQGVATGEELLRQVV
                     QTPPDLVILDVWLPGMDGIAALEEVKRLHPELPVIMMSGHGTVETAVKATKLGAYDFI
                     EKPLSLEKTILAVRNALDRQRLEQENLALRQTLEERYEIVGECPAIQTLRVQIQSAAP
                     SHGRVLIRGESGTGKELIARAIHRQSLRADKPFVEVNCAAIPDELIESELFGHEKGAF
                     TGATNKRRGKFEVADGGTIFLDEVGDMSLKTQAKVLRVLQEQTLERVGGTETLTVDVR
                     VIAASNKPLEEEIQKGSFREDLFYRLNVIPFEVPPLRDRKEDIPLLAAHFLRLFSREY
                     GKHPRSLCAEAMDLLVQYPWPGNVRELRNVMERLVIMVPQDPIQASDLALSLRIRMGA
                     PAETPAEPVLDWDGTLREARERFEREFILRRLGETHWNITRAAERLGLERSNLHRKIK
                     AYGIEMPRD"
     gene            complement(1680..>2344)
                     /locus_tag="AC16G0_16415"
     CDS             complement(1680..>2344)
                     /locus_tag="AC16G0_16415"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5101240.1"
                     /translation="ARRIAHEIKNPLTPIQLSTQRLRKKFAEKAPDVGLILDECTRTI
                     IQEVEGLRNLVDEFSRYARMPSLRPRPGDLHAVIRQVAQLYAGMRHGIRLRTDLDPGV
                     PTLNLDPDHMKRALINLVDNAVAAIADEEGEVAISTRFLPRSGQVVLEVADTGPGFPP
                     EDRDRVFLPYYSTKRSSGGLGLAIVQRIILEHGGQIHIDENQPRGARLIIELPVLAPV
                     AA"
BASE COUNT          390 a          788 c          723 g          443 t
ORIGIN      
        1 ccggtcgggg agcaggcgct ccagcacctc ccgatcggaa tacacgatgt agttcgtgta
       61 gtagtcccgg tccgtcaccg tcacgccata ggaccccagg acctcctgga aggcttgaca
      121 gtggagcggc tcgctgttga tcagcacgcc gtccaggtcg aagatcacac cccgctcatc
      181 gcgcatttcc tgcagctctc agcgttcagc actcagccat caaccagcag ccatgagcct
      241 cgaacccgcc tcaatcgcgg ggcatctcga tgccgtaggc cttgatcttg cgatgcagat
      301 tgctgcgctc gagaccaagg cgctccgccg cccgggtgat gttccagtgg gtctctccca
      361 gccggcgcag gatgaactct cgttcaaatc gctcccgagc ctcgcgcaac gtcccatccc
      421 agtccaagac cggctcggct ggagtctcgg ccggagcccc catacggatg cgaagtgaga
      481 gcgcgaggtc ggacgcctgg atgggatcct gcggcaccat gatgaccaag cgctccatga
      541 cattgcgaag ctcgcggacg ttccccggcc acgggtactg caccaacagg tccatggctt
      601 cggcgcacaa cgatcgcggg tgcttcccgt actcccgcga gaacaggcgg aggaaatggg
      661 cagccaggag cgggatgtcc tccttgcggt cgcgcagcgg tgggacctcg aaggggatga
      721 cattgagtcg gtagaatagg tcctcgcgga aactcccttt ctggatttcc tcctccaggg
      781 gcttgttcga cgcggcgatc acccggacat ccacggtgag ggtctccgtc ccgccgaccc
      841 gctcgagcgt ctgctcctgc aagacccgca ggaccttggc ctgggttttc aggctcatgt
      901 cccccacttc atccaggaag atggttccgc cgtcggccac ctcgaacttg ccgcgccgct
      961 tgttggtcgc cccggtgaag gcacccttct cgtggccaaa cagctcgctc tcgatcaact
     1021 cgtccgggat ggcggcgcag ttgacctcca caaagggctt gtccgcgcgc aggctttgcc
     1081 ggtgaatggc gcgggcgatg agttcttttc cggtcccgct ctccccccgg atcaggaccc
     1141 ggccgtggct gggcgccgcc gactggatct ggacgcgcag ggtctggatg gccgggcact
     1201 cgcccacgat ctcgtaacgt tcctccagcg tctgacgcaa ggccagattt tcctgctcca
     1261 ggcgctgccg gtccagggcg ttgcgcaccg ccaggatcgt cttctccagg ctgagcggct
     1321 tctcgatgaa atcgtaggcc cccagcttgg tggctttcac cgccgtctcc accgtgccgt
     1381 ggccggacat catgatcacc ggcaattcgg ggtgcagcct tttcacctcc tccagggcgg
     1441 caatcccatc cattcccggc agccagacgt ccagaatcac caggtcgggc ggcgtctgga
     1501 cgacctgcct cagaagttct tcacccgtgg ccacaccctg cacgcgatag ccctcgtccc
     1561 tgaggatgcc ctccagggac ccccgaatat tcttctcgtc gtcgacgacc aggatctctt
     1621 cgtggctcat gtttgttcag gcccgccgtg gagcagccgt gaaaccccct cggtcggggt
     1681 caggcggcga caggggcgag caccggcaac tcgatgatca gccgtgctcc ccgtggctgg
     1741 ttctcgtcga tgtggatttg tccgccatgc tccagaatga tacgctgcac gatcgccaga
     1801 cccagacccc cggacgagcg cttggtcgag tagtacggga ggaacacacg gtcgcgatcc
     1861 tctggcggga agccggggcc ggtatcggcg acctccagga cgacctggcc actccgcggc
     1921 aggaatcggg tggaaatcgc gacctccccc tcctcgtcgg caatggcggc cacggcgttg
     1981 tccaccaagt tgatgagggc tcgcttcatg tggtccgggt ccaagttgag cgttgggacc
     2041 ccgggatcca gatcggtgcg cagcctgatc ccgtgccgca tgcccgcgta gagctgcgcc
     2101 acctgccgga tcacggcatg caggtcgccg ggacgaggac gcaggctggg catgcgggca
     2161 taccgcgaga actcatccac caggtttcga aggccctcca cctcctggat gatggtccgg
     2221 gtgcactcgt ccaggatcag cccgacatcg ggcgctttct cggcgaattt ctttcttagt
     2281 cgctgggtgg agagctggat cggagtgagc gggttcttga tctcgtgggc gatgcggcgc
     2341 gcga
//
LOCUS       DBNGZC010000949         2125 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_568608, whole genome shotgun sequence.
ACCESSION   DBNGZC010000949 DBNGZC010000000
VERSION     DBNGZC010000949.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_568608"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..405)
                     /locus_tag="AC16G0_16420"
     CDS             complement(103..405)
                     /locus_tag="AC16G0_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HIR5101241.1"
                     /translation="MKVIVDLCVVPLGVGVSVSRYVAACERILNDAGLKTQLHAYGTN
                     IEGEWDAVFGAIKRCHEAVHEMGAPRISTILKLGTRTDREQTMDDKVKSVQSKLRE"
     gene            complement(829..>2125)
                     /locus_tag="AC16G0_16425"
     CDS             complement(829..>2125)
                     /locus_tag="AC16G0_16425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101242.1"
                     /translation="GNGLATDNLGIQVATQSDDPLHGQVPAGVSIEYAFDLNILRLHD
                     KRHGTAYGCDKDSRNCLEKQTPVSCQRCHYSPALDLAHVGPKGPGNPDANGRQQTLHQ
                     SMSRVIHNFHATLTNPDGTPLFPAMPSPVGRATATRDTFLARTCYQCHPGQVTKCFRG
                     VMFGAGAACQDCHGNLAQVGNDFSKNVSPTSPGTFVLAADYYTNPATPRVPWANEPAC
                     QSCHTGDVNSNLANSAGVLKASDGIRLLQAYRTTDPDAKPIIATNRRFAENVINGRQV
                     LYRLSKGHGGVFCEACHGSTHAEWPNPVPAANDNVTPTQLQGFAGKLIDCTVCHGTGL
                     FSVTTFRTLDANGLMPGPHGMHPLDVNWNRNHKEVFERVPRGTCRACHGVNLQGSVLA
                     RLPVTRTLICDEARNCRNGRITLAAGTLISCTLCHERPD"
BASE COUNT          338 a          638 c          730 g          419 t
ORIGIN      
        1 aggcggcgat ccagcgcttc gccgttcgtt gacccgacgc gtcccattcc gtaccagtgg
       61 atgatccggg ccccattcgg acgggacgtc cgggcccaag gattattcgc gcagcttgga
      121 ctggacgctc ttcaccttgt cgtccatcgt ttgctcccgg tccgtacgcg tccccagctt
      181 gaggatcgtg gatatgcggg gagcacccat ctcgtgtact gcttcatgac agcgcttgat
      241 cgccccgaac actgcgtccc actccccctc gatgttggtg ccgtatgcat gcaattgcgt
      301 cttgagaccg gcgtcgttca gaatgcgctc gcaggccgcg acatacctgg acaccgagac
      361 gcccacaccc agaggtacca cgcacagatc cacgatgacc ttcattcgtt ccgctccttc
      421 tcgaaatgct cctgaatccg ggtatgcgcc gaacgccact cgacgctcca ccccgcgtct
      481 gcttcgtcgc atgagcgtcg gtaccaccgg ctgccgcagt atgccgtcac gcactctagc
      541 gcattccttc ctcgggacca agacaaagcc agcattgtcc aatcgtgcga acacatgtct
      601 cggttaatac gcaggagacg tggtgaaccc tctactggcg gccctgacga ttgccggcga
      661 gcgaccgcag ggatccaggt ttggcgtgcg aggaggtgcc cgacgaatat gagaggagcg
      721 gtccgcgcgt gggttgggaa atcgaggacg gaacaggcgg cgcctcagtc ggatgaggga
      781 ctacctgttc cgtatgtaga gcctgtccgt ggatcgagtg gttcgccgct agtccggtct
      841 ctcgtggcac agagtacagc tgatcagcgt cccggccgcg agggtgatcc tgccgttgcg
      901 gcagttccgc gcttcatcgc agatgagggt gcgggtaacc gggaggcggg ccagcaccga
      961 cccttggaga ttgaccccgt ggcacgcccg gcaggtcccg cgcgggactc gctcgaagac
     1021 ctccttgtgg ttccggttcc agttgacgtc cagcgggtgc atgccatggg ggcctggcat
     1081 gaggccgttc gcgtccaatg tccggaaggt tgtgacggag aacaggccgg tcccgtggca
     1141 gaccgtgcag tcgatgagct tgccggcgaa tccctggagc tgggtcgggg tcacgttgtc
     1201 gttggccgcc ggcacggggt tgggccactc agcgtgggtg cttccgtggc acgcctcgca
     1261 gaataccccc ccgtgtccct tgctcaggcg atagagcacc tgcctgccgt tgatcacgtt
     1321 ctcggcgaat cggcggttcg tggcgatgat gggtttggcg tcggggtcgg tggtccggta
     1381 tgcctggagt agccggatgc cgtcggacgc cttgaggacc ccggcggagt tggcgaggtt
     1441 gctgttcacg tcacccgtgt ggcacgactg acaggcaggc tcgttggccc aggggactcg
     1501 tggcgtggcg gggttcgtgt agtaatcggc cgccaggacg aaagtgcctg ggctggtcgg
     1561 ggacacgttc ttcgagaagt cattgcccac ctgggcgagg ttcccgtgac agtcttgaca
     1621 ggccgcgccc gccccgaaca tgacgccgcg gaaacacttg gtcacctgcc cggggtggca
     1681 ctggtagcag gtgcgggcaa ggaaggtatc ccgggtggcg gtggcgcggc ccaccggcga
     1741 cggcatggcc gggaacagcg gggtgccgtc cgggttcgtg agggtcgcgt ggaaattgtg
     1801 gatgacgcgg gacatgctct ggtgcagcgt ctgctggcgc ccgttggcgt cgggattgcc
     1861 ggggcccttg gggccgacgt gggcgaggtc cagggctggt gaatagtgac accgctggca
     1921 ggagaccggc gtttgctttt ccaagcagtt gcgagagtct ttatcgcaac cataggcggt
     1981 gccatgccgt ttgtcgtgca ggcgcaggat gttgaggtcg aaggcgtact cgatgctcac
     2041 acccgctggc acttgaccat ggagggggtc gtcggattgc gtggccacct ggatgcccag
     2101 attgtccgtc gcgaggccgt taccg
//
LOCUS       DBNGZC010000950         2346 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1742451, whole genome shotgun sequence.
ACCESSION   DBNGZC010000950 DBNGZC010000000
VERSION     DBNGZC010000950.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1742451"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /locus_tag="AC16G0_16430"
     CDS             <1..170
                     /locus_tag="AC16G0_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008024819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HIR5101243.1"
                     /translation="TLLGRPAGFGRKIGDIVYRSIVESINVPPADNFQVIVEVKKENW
                     SFGNGVAQYAD"
     gene            complement(225..2054)
                     /locus_tag="AC16G0_16435"
     CDS             complement(225..2054)
                     /locus_tag="AC16G0_16435"
                     /inference="COORDINATES: protein motif:HMM:NF033559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HIR5101244.1"
                     /translation="MFIKPTKKKVGAKTYVNHVLVESVATPNGPRHRTVCSLGSLAPA
                     PAEEWLALAHKIEVALTGQQPLLRDARVDTLIAKVRAARRGARRSAGQSDLVAVHTDQ
                     VTVEEPREAGPVHVGHQLWRRLDLDVVLARAGLSPRTRVLTELMTLNRLVCPLSEHAM
                     PDWVRRTALADLLGEDFDTLVDESLYQNLNRLHPQRVAIEGALAERERTLLNLDARLF
                     LYDLTSTYFEGLCGQNPQAQRGYSRDRRPDCKQVVVGLVLDRDGFPKAHEVFEGNRPD
                     RTTVPEMLEALEERVGRTPGATVVVDRGMAFPDNLLQIQARGYHYLVASRPGERNDHL
                     AEFEQEAGWQEIVRAPSPTNPGQRKTRVLVKRKVVGDEVHVLCRSDGREAKERAIREK
                     HEQRLLADLRKLQARVATGKLKDAIKVHQAIGRLKERHPRVARYYAIGYDAATAAVTW
                     AEDVEKKAVAQRLDGGYLLKTDRQDLTDEEIWRTYILLTRVEAAFRAMKSPLCERPIF
                     HRLEHRVQTHIFLCVLAYHLLVAIEKLFLDAGIHTSWATLREELRTHQVVTVVLPASN
                     GDVLKIRRGSTPEPRHREIYATLHLPAEVMKPVKTWVRGIVTE"
BASE COUNT          365 a          805 c          766 g          410 t
ORIGIN      
        1 cgaccctgct gggaaggccg gcgggtttcg gccggaagat tggagacatc gtgtaccggt
       61 ccattgtgga gagtatcaac gtcccgccgg ccgataattt ccaggtcatc gtggaagtca
      121 agaaggagaa ctggtcgttc ggaaacggcg tcgcgcaata cgccgattaa cccaacttcc
      181 aatgtgggct gctgtcgatc cggcagtttc ggggggttac cttgtcattc agtgactatc
      241 cctcgcaccc aggtcttcac cggcttcatg acctccgcgg ggaggtgcag ggtcgcgtag
      301 atctccctgt gccggggttc cggcgtcgac ccccggcgga tcttcagcac gtccccgttg
      361 ctcgcgggca agaccaccgt cacgacctgg tgcgtcctga gttcctcgcg gagcgtcgcc
      421 caggaggtat ggatgcctgc atccaggaag agcttctcga tggccaccag caggtggtag
      481 gccagcacgc agaggaagat gtgggtctgc acccggtgct ccaagcgatg gaagatcggc
      541 cgctcgcaga gcgggctctt catcgcccgg aacgccgcct cgactcgcgt gagcaggatg
      601 taggtccgcc agatctcctc gtcggtgaga tcctgccggt cggtcttgag caggtacccg
      661 ccgtcgaggc gctgggcgac cgccttcttc tccacgtcct cggcccaggt gaccgccgcc
      721 gtcgcggcgt cgtacccgat tgcgtagtag cgggcgaccc gggggtggcg ctctttgagg
      781 cgcccgatcg cctggtgcac cttaatcgcg tccttgagct tccccgtggc cacgcgcgcc
      841 tggagcttcc gcagatccgc cagcaaccgc tgctcgtgct tctcgcggat cgcccgctcc
      901 ttcgcctccc gcccatcact ccggcacagg acgtggacct cgtctccgac caccttccgc
      961 ttcaccagca cgcgggtctt ccgctgcccc gggttcgtcg gggacggggc gcgcacgatc
     1021 tcctgccacc cggcctcttg ttcaaactcg gcgaggtggt cgttccgctc cccgggccgg
     1081 ctggccacga ggtagtggta gccgcgcgct tggatctgga gcaggttgtc ggggaaggcc
     1141 atcccccggt ccaccaccac cgtcgccccc ggggtgcgcc ccacccgctc ctccagcgcc
     1201 tccagcatct ccgggacggt ggtccgatcg gggcgattcc cctcgaacac ctcatgcgcc
     1261 ttggggaacc cgtcccgatc cagcaccagg cccaccacca cctgcttgca gtccggccgg
     1321 cggtcccgcg aatacccccg ctgggcctgc ggattttgcc cgcacagccc ctcgaagtac
     1381 gtggaggtca gatcgtacag gaagaggcgg gcatccaggt tgagcagcgt ccgctcccgc
     1441 tcggccaggg cgccctcgat cgcgacgcgc tgcgggtgca gccggttgag gttctggtac
     1501 agagactcgt ccaccagggt gtcgaagtcc tcccccagca gatcggccaa ggcggtgcgc
     1561 cggacccagt cgggcatggc atgctccgac agcggacaca cgaggcggtt gagcgtcatg
     1621 agctccgtga gcacccgtgt gcggggcgac aggccggccc gggccagcac gacgtccagg
     1681 tcgagcctcc gccagagttg gtgccccacg tgcaccggcc ccgcctctcg cggctcctct
     1741 accgtcacct ggtcggtgtg caccgcgacg agatcggact gccccgcgct ccggcgggcc
     1801 ccccgccgcg cagcccgcac cttcgcgatg agggtgtcca cccgcgcgtc gcggagcaag
     1861 ggttgctggc ccgtgagcgc gacctcgatc ttgtgcgcga gcgctaacca ctcctccgcg
     1921 ggggcggggg ccaggctgcc cagggagcac acggtgcggt gtcggggccc gttcggggtc
     1981 gccaccgact cgaccaggac gtggttgacg taggtcttgg cgccaacctt cttcttggta
     2041 ggcttgataa acatcgtgcc tacatatacg cttcagtgcc gagatgtcaa ggggaatagc
     2101 gcgacacgtg gccatttatt cgtgactatc tcgggctttc ggtgacgcgc ctcgcgaaaa
     2161 cattgagctt ttcgcccgcg cgaccttcaa aaacgccaac ccacattgga agttgggtta
     2221 gataccgttg tggagccgcg gaatcaggag gtccgcggcg gaggggggag ctatggcgac
     2281 cgcgaaacgg aaagtcggac cgaagccggg acagaagcgc caagtgggcc tccgggccag
     2341 ccgggc
//
LOCUS       DBNGZC010000951         3076 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2722234, whole genome shotgun sequence.
ACCESSION   DBNGZC010000951 DBNGZC010000000
VERSION     DBNGZC010000951.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2722234"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            383..2839
                     /locus_tag="AC16G0_16440"
     CDS             383..2839
                     /locus_tag="AC16G0_16440"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR5101245.1"
                     /translation="MTLQFGPRRGSYVDVTEILKAEKPPLSPEEIGHFETLDLIYRSL
                     CTLLYNYVPMSGHPGGSISSGRFVAGLLFDALSYDLAKPDREDADIISYAAGHKAMGL
                     YSFWAIRDEFARLAAPDLLPADVKFRLRLEDLLGFRRNPITKTPLFKQFGAKARDGHP
                     TPATPFIRLSTGASGVGVASSLGLAFGAKDYFGAAAPLVHIVEGEGGLTPGRAAEALA
                     GAGTASLGNAILHLDWNQASIDSNRVCREDDKPGDYVQWTPMELFHLHDWNVIYVPDG
                     KDFQQIFAAQRAALAIANGQPTAVVYRTVKGWQYGIEGKAAHGAGHKLCSDGFCNALA
                     ELTSRADMMMPRCEAGNPLCQMSPNGAAIMEECFWESLLIVRKVIADSRPAVEAFAAR
                     MVAARDRLNRLNRHPRAGAPRVEAVYELAAKGADTPEELKLKPGTTTTLRAELGRTLQ
                     HYNKASGGAIFAGAADLLGSTSLNTAGAGFGEGYWNAVKNPNARLLSIGGICEDGMTG
                     ILTGLSTFGHHIGSGSSYGAFIAPLSHIAARLHAIGAQAKHAVTGQPYRPIFVVCAHA
                     GLKTGEDGPTHADPQPLQLLQENFPKGTAITLTPWDPQEIWPLVSAALAKRPAVIAPF
                     VTRPNEKVLDRAKLGLAPAEEAVNGVYLLRKPRGKGQGTIVLQESAATYAFLEEALPL
                     LEKEGLDLWVYYVSSAELFDLLPPAKQRRLFPEERAREAMGITGFTLPTMFRWVVSDL
                     GRSMTLHPFSKGHFLGSGQGEVVLAEAGLDGKSQFKSIKKYVEALRQATKQAPKVVRA
                     AARPVIRRKAQPKAKARKRR"
BASE COUNT          533 a         1067 c          972 g          504 t
ORIGIN      
        1 cttcgagtac gagacgccgg aggcggagga gacgggcatc ccgcgcgccc tggcctacct
       61 caagcggatg ctggctggat agccccggca tctcggatgg cgacccgctc cccagggtca
      121 cggcgacgac ctgaatctct ccaggaccgc cgggatgggc tcggcatccc ggccgggtcg
      181 agtcttgtga ccatttccac gcatttcgcg agaaatgatt gccacggggg gagcgatcgg
      241 tctaggctgt gcgggtcaat tgagggcgac attccatcca cccccggagc ccgaggaggc
      301 cttcctcggt gatcccggca tggactgccg ctgtccattc gaagtgagag acaccgaggc
      361 ttgacgtgaa aggaggagtc gcatgacgct gcagttcgga ccgagacgtg gaagctacgt
      421 ggatgtgacc gaaatcctca aggccgagaa gcctccgctg tcgcccgagg agatcggcca
      481 cttcgagacc ctggacctca tctatcgctc cctgtgcacc ctcttgtaca actacgtccc
      541 catgtcgggg caccctggcg gctcgatctc ctccgggcgg ttcgtcgccg gcctgctatt
      601 tgacgcgctg agctatgacc tcgccaagcc cgatcgagag gacgcggaca tcatctccta
      661 cgcggccggc cacaaggcca tgggcctcta ctcgttctgg gccatccggg acgagttcgc
      721 gcgtctggcc gcgcccgacc tgctccccgc tgacgtgaag tttcgccttc gtctggagga
      781 cctgctgggc ttccgccgca accccatcac caagaccccg cttttcaagc agttcggcgc
      841 caaggcgcgg gatggccatc ccaccccggc aacaccgttc atccgactct cgaccggggc
      901 ctccggcgtc ggcgttgcca gctcgctggg gctggccttt ggcgccaagg attacttcgg
      961 tgcggctgcg cccctggtcc acatcgtcga gggcgagggc ggcttgacgc ccggccgcgc
     1021 ggcggaagcc ctggccggtg ccgggaccgc ctccctgggc aacgctatcc tccacctgga
     1081 ctggaaccag gcctccatcg attcgaatcg cgtctgccgc gaggacgaca agccaggcga
     1141 ctacgtccag tggaccccca tggagctgtt ccaccttcac gactggaacg tgatctacgt
     1201 ccccgacggc aaagacttcc agcagatctt cgccgcgcaa cgcgccgccc tggccatcgc
     1261 caacggccag cccaccgcgg ttgtctaccg cacggtgaag ggatggcagt acggcattga
     1321 aggcaaggct gcccacggcg ccggtcacaa gctctgctcc gacggcttct gcaatgcctt
     1381 ggccgagctg acgagccggg cggacatgat gatgccacgc tgcgaggccg gcaacccgct
     1441 gtgccagatg agcccgaacg gcgccgccat catggaggag tgcttctggg aatcgctcct
     1501 gatcgtgcgc aaggtgatcg cggacagtcg tcccgccgtc gaggcgttcg ccgcccgcat
     1561 ggtcgccgcc cgcgatcggc tgaaccgtct gaaccggcat ccccgagccg gcgcgccgcg
     1621 agtcgaggcc gtgtacgagc tggccgccaa gggcgcggac actcccgaag agctcaagct
     1681 caagcccggc accaccacga cgctccgggc ggagctggga cgcacgctcc agcactacaa
     1741 caaggcatcc ggcggggcga tcttcgccgg tgccgccgac ttgctcgggt caacgagcct
     1801 gaacaccgcc ggcgcgggat ttggcgaggg gtactggaac gcggtcaaga acccgaacgc
     1861 ccggctgctg tccatcggcg gcatctgcga agacgggatg acgggcatcc tgaccggcct
     1921 gtccaccttt ggccaccaca tcggcagcgg ctcctcctac ggagcattca ttgcacccct
     1981 cagtcatatc gccgcccgac tccacgccat cggggcccag gcgaagcacg ccgtcacggg
     2041 gcaaccctac cgaccgatct tcgtggtctg tgcccacgcg ggcctgaaga ccggcgagga
     2101 cgggcccacc catgcggatc cccagccgct gcagttgctc caggagaact tccccaaggg
     2161 cacggccatc acgctgacgc cctgggatcc tcaggagatc tggcccctgg tgtccgcggc
     2221 gctggccaag cggcccgcgg tcattgcccc cttcgtgacc cgccccaacg agaaggtcct
     2281 cgaccgggcg aagctgggac tcgccccggc tgaagaggcg gtgaacggcg tctacctcct
     2341 gcgcaagccc cgcgggaagg ggcaggggac aatcgtcctc caggagagcg cggccaccta
     2401 cgccttcctc gaagaggcgc tgcctctgct ggagaaggag gggctggatc tctgggtcta
     2461 ctacgtgtcc agcgccgagc tgttcgacct gctcccgccg gccaagcagc gccgtctctt
     2521 ccccgaggag cgcgcccggg aagccatggg catcaccgga ttcaccctgc cgaccatgtt
     2581 ccgctgggtg gtgtctgatc tggggcgatc catgacgctc cacccgttct cgaagggtca
     2641 cttcctcgga agcggtcagg gtgaggtggt gctggccgag gcgggtctgg atggcaagag
     2701 ccagttcaag tccatcaaga agtacgtgga agcgctccgg caagcaacga agcaggcgcc
     2761 gaaggtcgtt cgggcggcgg ctcgtccagt tatccgccgg aaggcgcagc cgaaagccaa
     2821 ggcccgcaag cggcggtaga gtccccatcg tcgggggtcg aaccgtcaga acctgtttgg
     2881 ttctttcgcg cgcagcgcgc aagaaccaag ttccaacaag aagcgggcgg gagggctcaa
     2941 gcgccctccc gcccgcgtgt ttcccaaacc ttccgtgcac attcctctca taaccaccac
     3001 tgattctggc cacccttcct tctcgtctat aattgcaccc cgtgttactc gaacgggcgg
     3061 gctggtattg agattg
//
LOCUS       DBNGZC010000952         2420 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2233308, whole genome shotgun sequence.
ACCESSION   DBNGZC010000952 DBNGZC010000000
VERSION     DBNGZC010000952.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2233308"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..762
                     /locus_tag="AC16G0_16445"
     CDS             193..762
                     /locus_tag="AC16G0_16445"
                     /inference="COORDINATES: protein motif:HMM:NF017496.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HIR5101246.1"
                     /translation="MKRNVFTPTPVKVTLEEYLALPETTLPHELVDGDLRLTAAPSTW
                     HQTVLNRLARPLEDFVESRKLGLVFRAAVDVILDRLAGLVLQPDIIYIRQNRAVIVHE
                     RIEGAPDLVVEILSPRTRDYDRTEKSVRYAAAGVAEYWLADSENGTIEIRRLDQRPPL
                     TIAVFTSSDSLTSPLLPAFALPVAPIFAR"
     gene            complement(796..1089)
                     /locus_tag="AC16G0_16450"
     CDS             complement(796..1089)
                     /locus_tag="AC16G0_16450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101247.1"
                     /translation="MRAIPFKLDELLWPKHPLHIQGAAGTAPTLWALFGVGNAFELAE
                     EFGGHHTQLVSWHSGMLTTWRIAWVVASDNPRHITQPVNRRRETIACEGSPGQ"
     gene            1679..2023
                     /locus_tag="AC16G0_16455"
     CDS             1679..2023
                     /locus_tag="AC16G0_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101248.1"
                     /translation="MGQRGAVWWEVWTIAVVFLGVYIGFEVLDLDGSQLQLRSGVALT
                     VESASVEADRSLRVCPIPLAVRLQPYLLALHTMLPLRPVTRPGRPLGGKRLLPRRQLA
                     HVARLMQSADPV"
BASE COUNT          387 a          829 c          734 g          470 t
ORIGIN      
        1 cgcaacgttg gtgactctcg gcgaggaact agacttggac accgttttca ccctcgaccg
       61 tcgcggattc ggcgtgtacc gcctccgcgg tcgccggtcc ttccagattg ttccgtgagg
      121 ccatctatag tgtaccggga cgattccgag caagccattg accgcgtgat gtgaggtgcc
      181 agggtgcatg cggtgaaaag aaacgtgttc acgccgacac cggtaaaggt taccttggag
      241 gagtacctcg ccctccccga aaccacactc cctcatgagc tcgtggatgg agacctacgt
      301 ttgacggcag cgccgagtac gtggcaccaa acggtcctca accggttagc ccgcccgctt
      361 gaggatttcg tcgagtcgcg caagctcgga ctggtgttca gggctgccgt ggatgtcatc
      421 cttgaccgcc ttgcagggtt ggtgctccag cccgatatca tctacatccg ccagaatcgt
      481 gcagtcatcg tccacgaacg cattgaaggt gctcccgatt tggtggtcga gatcctatct
      541 ccgaggaccc gggactacga tcgcaccgaa aagtccgtac gctacgccgc cgcgggcgtc
      601 gcggagtatt ggctcgccga ttcagagaac gggaccatcg agatccgccg gttggaccaa
      661 cgaccgcccc tcaccatcgc cgtcttcaca tcctcggatt cgctgacgag cccactcctg
      721 cctgccttcg cccttcccgt cgccccaatc ttcgcccgat agccccacgc acctcttttc
      781 ttctgccctg ccccgttact gccccgggct tccctcgcac gcgatcgtct cccgtcgccg
      841 gttcacgggc tgggtgatgt ggcgcgggtt gtccgacgcc accacccagg cgatgcgcca
      901 tgtcgtcagc atcccactat gccagctcac gagttgcgta tggtgtcccc cgaattcctc
      961 cgccaattcg aacgcatttc ccactccgaa cagtgcccac aacgtgggtg ccgtccccgc
     1021 ggcaccctga atgtgcaatg gatgcttggg ccacagaagc tcgtcgagct tgaatggtat
     1081 cgcacgcatc gcgctgtcat ttgccaatac cccgcccagg cccctctcct ctcttgtagg
     1141 cggtttgaat cgaggatctc aaggtcattc cggtctgaag tagttggccc ggatcagccc
     1201 tgcggtcagg ctctctgaca gtcgtctcaa caggacgagt gggtcggtgg tacccgtcgg
     1261 atcgtttgcc ccccttgtga cgatcggacg gaggaggcac catggtctcg gccaggacca
     1321 gcacttcggc ctcgagacgg gcaggcagct cgatgcgccc gccactgttc gtccgtccgc
     1381 gtccctgttc ccccgccagc agcgagcccg acgtgtcctc cggatccctg ggagatcggc
     1441 taggctcccg cggcctcgac gcgttgcggt ggcatcaacg gcacggcctg gggtgcggtc
     1501 ccggggaccc cgtgaaatac gcgagccgcc gcccttgggg gcccccacga tccgcccgag
     1561 gagaacttgg tgccccaccg acggcacggg gaaacgcgca tagtcttcca gcttgacagc
     1621 cacgcaagag cggggctatt ctgcttctcg ttgcacccgg aagccgttga ggggtccgat
     1681 ggggcagcgc ggcgcggtgt ggtgggaggt ctggacaatc gccgtcgtct tcctcggcgt
     1741 ctacatcggt ttcgaggtcc tggacctcga cgggtcgcaa ctgcagcttc ggtccggcgt
     1801 cgcgctcacg gtcgaatctg cctctgtcga agccgaccgc agtcttcgcg tgtgtccgat
     1861 cccgctcgcg gttcgcctgc aaccgtacct gctcgccctt cacacgatgc tcccgcttcg
     1921 gcccgtgacg aggccaggtc ggcccctggg tggcaagcgc ctcctcccgc gccgacagtt
     1981 ggcacacgtt gcccgcctca tgcagtccgc tgaccccgtc taacccaccc gtcgtgcctg
     2041 cagtgcctct ctggcagggg cttcgcacgc gccgaagcag ccttgctcac cggggtttct
     2101 attcgggacg tccgcttgtc ctcgcaatcg cctcggggac gcacacgaaa gggggcagcc
     2161 atggggttcg ggttcggcat cagggtggcg atgatctcgg cctgatctgg tggtgcgtct
     2221 gggcgtatcg tagcgacagc gcgcggtagg aactccctct ggccgacgag caggcacgca
     2281 aagcggggta tgccaggcgc gagcacgggc agacccggac ttcccctcgt gcgggaacgc
     2341 ccgctcgcct cctccaccgc cgaagcgaga ggcggggcat aaatcggcga gtccgtgaac
     2401 tggctcggag ccggatgccg
//
LOCUS       DBNGZC010000953         5716 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1352248, whole genome shotgun sequence.
ACCESSION   DBNGZC010000953 DBNGZC010000000
VERSION     DBNGZC010000953.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5716)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1352248"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..5499)
                     /locus_tag="AC16G0_16460"
     CDS             complement(175..5499)
                     /locus_tag="AC16G0_16460"
                     /inference="COORDINATES: protein motif:HMM:NF024180.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HIR5101249.1"
                     /translation="MPTVRIPGGREEGGGSQTQRRADGKLTVTRRSAVRVAPARGADT
                     GAAKVKFTDEDVVALELEGGFRLWIRGDDFRKEFGAQAKRGVTDGEEEWELSPDLQVG
                     SPERGMGTWLLKGLEVFGVDLTEVAASTIAGHVEATLERGPGLYACNLSGGFALKDLP
                     PHLPTDRPLLVFLHGTASSTSGSFGGLWGNKSPMEVLARHYADAAFAFEHRTLTQSPI
                     QNALDLVTALPKGARLHLVSHSRGGLLGELLCRGQRGDRGDPFEGREKALFADRKEER
                     NAIEALNTALKDKQIRVDRFVRVACPARGTTLASGRLGRWLSVLVNLLGQIPALQGNP
                     LYEALTDFLLAVVKEGTDPKTLPGLESMMPDSPLIRVLNLPGVSVQSDLSVISGDIEE
                     AGVWGKLKLLIPDLFYGGDHDLVVNTGSMYGGAGREKGARFFLDQGPSVNHFHYFINE
                     KTVQMLVEGLTRPDGGQAGFSPIEKARQEVPARAVRGAAGPRPVVFLIPGIMGTHLAV
                     DGDRIWLDPFDLAFGGMGRLRVDATNVEAQALIAMAYGELVEYLSQTHEVVTFPFDWR
                     LSIRNEAERLARALEKALDSAERANQPVRLLAHSMGGLVARAMIAEHPEIWRRICQHP
                     AGRLIMLGTPNQGSYIIVKLLVGQEEMFRYLALLDITHSGTQLLEIISRYPGVLEMLP
                     ENGTPDFFSPAYWSGLKTQNQGEDWALPDQKGLQDAVASRQLIRNSPIDPDKMLYVAG
                     CAPATPCGLRISEKGEIEFLATGRGDGRVLWDTGIPAGVRTWYMEGVAHGDLANHEPA
                     FPALLELLQSGQTARLPAAPPVAARGVAETFPLPREVVPMLPDEDALAAAAVGSRRRT
                     TRKAPVREVRVSITHGNLGYARHAVAVGHYQGDTIVGAEAYLDKVLQGRLGRRQQLGL
                     YPGGLNTAEVILNPDSYGKPGGAIVIGLGRVGELSPGGLASTFARAALAYALAVAEAA
                     DARFQTAAGVPRSAKISSLLIGTGAGGFSVDESVNAILRGVLKVNTLLRESRQDTRVV
                     IEELEFVELWRDLAIQAGRSLERVEEDSELRGFFECEAKVRQGQGGSIRVQWQDDPHW
                     WHRLQIMGKEDGSLRFSALTERARAEVSLLPAQRALLDTFIQQAITSTARSPETTRTL
                     FELLVPNRLKEQAPRRYNLVLILDEDSARYPWELLEDRWGDASQPMAVQAGLVRQLET
                     AEYRDQVRMTTQETAYVVGDPVSKFVALPGAEAEARSVAEQFATRGYTVERQIRAEAR
                     AIVTSLHARAYRIVHLAGHGVHEEVLDDAKKLACDLCGQPRPDKAGQKVSGMVIGDDI
                     FLTPADVEQMRQVPELVFINCCHLGRTDSREPDTRRDDRHRLAANVAAEFIRMGVKAV
                     VAAGWAVDDGAAKTFAHKFYDAMLEGVPFGEAVRLARSETYQRHPGVNTWGAYQCYGD
                     PDYRLVVQDDWTGRAPEERPYVSSVEVVADLGNLTGEAKTAGDERLKALKARIDQILK
                     LADQAWLKQGDVAAALGQAYGELGQFDKAIEHLGRAVQAEKAEFPVRAVEQRANFKSR
                     WAVELFRSGQKPTGGVKPSDLIQEAVREVEALKQFGPTAERWSLLGSAHKRHAWMAEG
                     DERKGALKSMASSYRKAHELAYAKGQGRLDTYPLLNWLAAEILLDWYGTPHPAALAQI
                     EDWCQRAEAHASEREKADPDFWNSVVKPECDLVRALARKTLDREKDTIVDGYRRAKAR
                     GASPREFRSVLEHLEFLVHVAIGGGPGDIQTQAEALRAITDQLSSEAG"
BASE COUNT          897 a         2009 c         1817 g          993 t
ORIGIN      
        1 gcaggactgc acggcgatac agccacagga ggagggccga ggtgggcgcg gtgagcgcgg
       61 ccgcgatcaa ggtgatggcg ggaatcatgc ccgtgagcgc gaccgtggta ttcacggcgt
      121 acccctagta aactggatgg ctgagcaggc tacgcgcggc atccagctct cctttcatcc
      181 tgcctcgctc gagagctgat ctgtgattgc ccgcaacgcc tcggcctgcg tctggatatc
      241 gcctggcccc ccgccgatgg cgacgtggac cagaaactcc aggtgctcca ggacggagcg
      301 gaactcccgc gggctggcgc cccgcgcctt ggcccgccga tagccgtcca caatcgtatc
      361 cttctcccgg tcgagggtct tcctggcaag cgctcggacc agatcgcatt ccggcttgac
      421 cacgctgttc cagaaatcgg gatcggcttt ttccctctcg gatgcgtgag cttcggccct
      481 ttgacaccag tcctcgatct gggcgagagc ggccggatgc ggagtcccat accagtcgag
      541 gaggatttcg gccgccagcc agttgagcag agggtatgtg tccagcctgc cttggccctt
      601 tgcataggcg agctcatggg ctttcctgta agaactcgcc atggatttca gcgcaccttt
      661 acgctcgtcg ccttcagcca tccaggcatg ccgcttgtgg gcgctgccga gcagactcca
      721 ccgctccgcc gtgggaccga actgcttgag agcctccacc tcgcgaaccg cttcctggat
      781 cagatcggaa ggcttcaccc cgccagtggg cttctggcca ctgcgaaaca actccacggc
      841 ccaccggctc ttgaagtttg cccgctgctc cacggcccga accgggaatt ccgccttctc
      901 ggcttggaca gcccggccta gatgctcgat ggccttgtcg aactggccca attccccgta
      961 cgcttggccc agggccgcgg ccacatcgcc ctgtttcagc caggcctggt cggccagttt
     1021 cagaatctgg tcaatcctgg ccttcagggc cttcagccgt tcgtcccccg cagtcttggc
     1081 ctcgccggtc aggttcccca ggtctgccac cacctcgaca gatgagacat agggtcgttc
     1141 ctccggggcg cggccggtcc agtcgtcctg gaccaccagt cggtagtcgg ggtcgccgta
     1201 gcactggtag gcaccccagg tgttgacgcc gggatggcgc tggtaggtct cgctgcgggc
     1261 cagacgcacg gcctcaccaa agggcacacc ctcgagcatg gcatcgtaga acttgtgggc
     1321 gaatgtcttg gccgccccgt catccactgc ccagccggcg gcgacaacgg ccttcacccc
     1381 catgcggatg aattctgcgg ccacgttggc ggctaacctg tgccgatcgt cgcgccgggt
     1441 gtccggctct ctcgaatcgg tccgccccag gtggcaacag ttgatgaaga ccagctccgg
     1501 cacctggcgc atctgctcca catcagccgg ggtcaggaag atgtcgtcgc cgatcaccat
     1561 cccgctgacc ttctgccccg ccttgtccgg gcgcggctgg ccgcagaggt cacaggcgag
     1621 tttcttggcg tcgtccagga cttcctcgtg caccccgtgt cccgccaggt gcacgatgcg
     1681 gtaggccctg gcgtgcaggc tggtgacgat tgctcgggcc tcggcgcgga tctgtcgctc
     1741 cacggtatag ccgcgtgtgg caaactgctc ggccacgctc ctggcttccg cctcggcgcc
     1801 gggcagcgcc acgaacttcg acaccgggtc ccccacgacg tacgccgttt cctgcgtggt
     1861 catgcgcacc tggtcgcggt actccgcggt ctcgagctgg cggaccaggc cggcctgcac
     1921 cgccatcggc tggctggcat cgccccagcg atcctccagc agttcccacg gatagcgggc
     1981 agagtcctcg tcgaggatga gcaccaggtt atagcgcctg ggcgcctgct ccttgaggcg
     2041 gttgggcacc agcaattcga acagggtccg cgtcgtctcg gggctgcggg cggtggaggt
     2101 gatggcctgt tggatgaacg tgtccagcag cgcgcgctgg gccggcaaga ggctcacctc
     2161 ggcgcgagcc cgttccgtga gggcgctgaa ccgcagcgag ccgtcctcct tgcccatgat
     2221 ctggaggcga tgccaccaat gggggtcgtc ctgccactga acccggatgg agccgccctg
     2281 gccctgccgt accttggcct cacactcgaa aaatccccgc agctccgaat cctcctcgac
     2341 cctttccagg gaccgccccg cctggatggc caggtctcgc cacaactcca cgaactccag
     2401 ctcctcgatg acgacccgtg tgtcttgacg cgactcccgg agcaaggtgt tgaccttgag
     2461 cacgccgcgc aggatggcgt tcaccgactc gtctaccgaa aagccgccgg cgccggtgcc
     2521 gatcaggagg gaactgatct tcgccgagcg tggtacgccg gccgcggtct gaaagcgcgc
     2581 atccgcagct tccgccaccg ccagggcata ggccagggcc gcgcgggcga aggtcgaggc
     2641 caggcccccc gggctcagct ctccgacccg gcccagcccg atcacgatgg cccctcccgg
     2701 cttgccgtat gagtcggggt tgaggatgac ctcggcggtg ttcagcccgc ctgggtagag
     2761 gcccagttgc tggcgtctgc ctagccggcc ctggagcact ttgtccaggt aggcctctgc
     2821 gcccacaatg gtgtcgccct ggtaatgtcc gaccgccacg gcatggcggg cgtagccgag
     2881 attcccgtgg gtgatggaga cccggacctc gcgaacgggc gccttgcgcg ttgtgcgccg
     2941 acgggagccg acggcggccg ccgccagggc atcctcgtcg ggcagcatgg ggaccacctc
     3001 ccgcggcagc gggaaggtct ccgccactcc cctggcggct actggcggcg cggccggcaa
     3061 gcgggccgtt tggccgctct ggagcaactc cagcagggca gggaaggcgg gctcgtgatt
     3121 cgccaggtcg ccgtgcgcca ccccctccat gtaccaggtc ctcaccccgg ccggaatccc
     3181 cgtgtcccag agaacccggc catccccgcg gcccgtggcc aggaactcaa tctccccctt
     3241 ctccgaaatc cgcaggccgc agggtgtggc gggagcgcag cccgccacat acagcatttt
     3301 gtctggatca atgggactgt tcctgatcaa ctgccgggat gccaccgcat cctgcaaccc
     3361 cttttggtcc ggcagcgccc aatcctcacc ctggttctgg gtcttgagtc cactccagta
     3421 cgccggactg aagaaatcgg gagttccatt ctccggcagc atttccagaa cacccggata
     3481 gcgggagatg atctccagga gctgcgtccc gctgtgggtg atgtccagca gcgccaggta
     3541 gcggaacatc tcctcctgtc cgaccaacag cttgacgatg atgtaggagc cctggttggg
     3601 cgtcccgagc atgatcaagc ggccggctgg gtgctggcag atccgcctcc agatttccgg
     3661 atgctcggcg atcatggcgc gggccaccag tccgcccatg gagtgagcca ggaggcgcac
     3721 cggctggttg gcccgttccg ccgagtccag tgccttctcc aaggccctgg ccagtcgctc
     3781 cgcctcattc cggatggaga ggcgccagtc gaacgggaag gtgaccacct cgtgggtctg
     3841 gctcaggtac tcgaccagct ccccgtaggc catggcgatg agagcctggg cttccacgtt
     3901 ggtcgcgtcc accctgagcc gccccatccc gccgaaggcc aggtcaaagg gatcgagcca
     3961 gatgcggtct ccgtccacgg ccaggtgggt gcccatgatg cccggtatca ggaagacgac
     4021 aggccgcggg cccgcagcgc cgcgcacggc gcgggcggga acctcctgac gggccttctc
     4081 gatgggggag aatccggcct ggccgccgtc cggccgggtg agcccctcca ccagcatctg
     4141 cacggtcttc tcgttgatga agtagtgaaa gtggttgacg ctgggtccct ggtccagaaa
     4201 gaagcgcgcg cccttttcgc gtccggcgcc gccgtacatg gagccggtgt tcaccaccag
     4261 gtcgtggtcg ccgccataga acaggtcggg gatgaggagc ttgagcttcc cccacacacc
     4321 cgcctcctcg atgtcgccgg agatcacgga caggtccgac tgcacgctca ctcccgggag
     4381 gttgagcact cggatcagcg gtgagtcggg catcattgac tcgaggccgg gcagcgtctt
     4441 ggggtcggtg ccctccttga ccactgccag caggaaatcg gtcaaggcct cgtagagcgg
     4501 attgccctgc agggcgggga tctgacccag gaggttcacc agcacggaga gccagcggcc
     4561 gagccggccc gaggccaggg tcgtcccccg cgccgggcag gccacccgca cgaagcggtc
     4621 caccctgatc tgcttgtcct tgagcgcggt gttcagggcc tcaatggcgt tacgctcctc
     4681 cttgcggtct gcaaacaggg ctttctccct gccctcgaag ggatctcccc tgtctccccg
     4741 ctggccgcgg cacaggagct cgcccaggag gcccccacgg gagtgggaga ccaggtgaag
     4801 cctcgcgcct ttcggaagag cggtgaccag atccagggcg ttctggatcg ggctctgggt
     4861 gagggttcga tgctcgaagg cgaaagcggc gtcagcgtag tgccgggcca agacctccat
     4921 gggtgacttg ttgccccaga gcccgccgaa gctgcctgag gtgctcgagg cagtgccgtg
     4981 gaggaagacg agcagcgggc gatccgtagg caggtggggt ggcaggtcct tgagggcgaa
     5041 gcccccggag agattgcagg cgtaaaggcc cgggccgcgc tccagcgtgg cctcgacgtg
     5101 ccccgcaatc gtgctggccg caacctctgt caggtccaca ccgaagacct ccaggccctt
     5161 gagcagccag gtccccatcc cccgctccgg gctgcccacc tgaaggtccg ggctcagctc
     5221 ccattcctct tccccgtccg tcacgcctcg cttggcctgg gcaccgaact ccttccggaa
     5281 gtcgtcgcct cgaatccaca gcctgaatcc gccctcgagc tccagggcga caacatcctc
     5341 gtcggtgaac ttgaccttgg ccgctcccgt gtcagccccc cgggccggag ccactcgcac
     5401 ggcgctccgg cgcgtgaccg tgagcttgcc atccgcgcgc cgctgagtct ggctgccccc
     5461 gccttcctct cgcccacccg gaattctcac ggtcggcatg gctggctcct ctccccctga
     5521 ccacacccga gatttcgaaa gcaacccctg cgcggaccgg tcctcctcaa cccgcagcca
     5581 cgcgcgtcag gaccaagaag gctcggatct tccgtgggtc cagcctgggt gctcggcgcg
     5641 ccgtatccca tgccagggcc gatccccgct accgccccag gcccgccaga taggccgtca
     5701 ggaactcgtc ccaggc
//
LOCUS       DBNGZC010000954         4434 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2523756, whole genome shotgun sequence.
ACCESSION   DBNGZC010000954 DBNGZC010000000
VERSION     DBNGZC010000954.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2523756"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="AC16G0_16465"
     CDS             <1..259
                     /locus_tag="AC16G0_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101250.1"
                     /translation="NNCLAAHERLSYSTNYPITQDWWTIPDGNCSRAAELTLANGEKS
                     HIPANVSTGTTTVNGKTVPYRIDATTCKNTVAVVPPHTIEP"
     gene            430..1449
                     /locus_tag="AC16G0_16470"
     CDS             430..1449
                     /locus_tag="AC16G0_16470"
                     /inference="COORDINATES: protein motif:HMM:NF048868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virginiamycin B lyase family protein"
                     /protein_id="HIR5101251.1"
                     /translation="MTTIGRSAGGGASRHLWFLVATGSLVVIALGSLVGVVRPWAPLG
                     FVETRMASRTDVPISIAAAPDGTIWFTLEGAVGRLRNGAIEKIPRRMADIEPLGLAVD
                     AGGAVWFTDTPMRAIGRLDVDGGSQSFRLPMSMVRLGRLAVAPDGTVWFAEPSTVSVT
                     RLRDGVFTRYLVGTPAPMGSTNVAPFGVAVDPHGTVWATLQNANQLVRIAPGNQMITF
                     EVPTSRSGLGDVAVDAVGAVWFLELAANKVGRYASGRFQEFTVPTPSAGLTALAVAPD
                     GAAWFTELRAHKLGRVYRGTITEFPLPREDARPFGLAVDASNNVWYTDLSGWLGHLPA
                     DRARG"
     gene            2258..2764
                     /locus_tag="AC16G0_16475"
     CDS             2258..2764
                     /locus_tag="AC16G0_16475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR5101252.1"
                     /translation="MPAIVVETLALDPSDPARVWAAGTGRVFRSDDSGKHWQSVGRPL
                     PTANTAVHGIAATGSSVVVSTDRGLYRSSDGGELWQLVTDNVPAHLEAGPLVRDPVDP
                     ATVYAGFAATPYAELWRRATESHASLWQVNSQKLGTATVLVIFLALDAVFGAQWLMRR
                     YRTPRQQH"
     gene            2873..4204
                     /locus_tag="AC16G0_16480"
     CDS             2873..4204
                     /locus_tag="AC16G0_16480"
                     /inference="COORDINATES: protein motif:HMM:NF025167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HIR5101253.1"
                     /translation="MTHTLGKAVAVLAFVTAVSISGTAGAHNVPGGMVMNAFVQLEGT
                     RALLVIRVPGHLLAEMSIPTDVTGASGPERGGAAMSRALTAITEGITIRERGVPLVPI
                     GAVGRLTLSSDRSFENYEEAVAHVAGPTPPQLATSAELAFLDAHITYQIADPGSDYTI
                     QTGMLPQLGESVKLVVRFIRPDGSIRPYEITSLSGPVPLDPHWYQAARVFVTAGFFHI
                     LDGIDHLLFLLCLVIPFDNLWALLPIITSFTVAHSVTLIGSAYGAVPKGEWFPPLVET
                     LIAMSIVYMALENIIRVNLRRRWLITGAFGLVHGFGLSYGLAQTLQFAGSHLLLSLLS
                     FNVGIELGQLAVLLLTLPALRLLLGQLLPGRPGRMALSSMVAGTGLYWMFERAQVLWR
                     VPWPVPNAAASVTAGRWVLLVLLMAGAGWVSRKSLRRHAAGPGPSTKEDIA"
BASE COUNT          743 a         1414 c         1445 g          832 t
ORIGIN      
        1 caacaactgt ctggcggccc acgagcggct gtcctactcc accaactacc ccatcacgca
       61 agactggtgg accatccccg acgggaactg ctcgcgtgcc gcggagctga cgctggcgaa
      121 tggggagaaa agccacatcc cggctaacgt ttcgaccggc acgaccacgg tcaatgggaa
      181 gacggttccc tatcggatag atgcaaccac gtgcaagaac acggtggccg tggtcccacc
      241 gcacacaatc gaaccatagc gagcccacgg gtgaagcctg gcctggaacc cgccgccggt
      301 tccaggccag ttgctcggtc ccgccagttt ccagaggtat gtaactcccc gacggaattt
      361 gaagggggcg ctgatgatcg ccaggcaaga atcggtgaca cgatcgaccg gatcctcggg
      421 agcgcattaa tgaccaccat cggacgcagc gcaggtgggg gggcgagtcg acatctctgg
      481 ttcttggtgg ccacggggag cctggtcgta atcgcgctgg ggtcgcttgt gggggtcgtc
      541 aggccttggg cccctctagg ctttgtcgag acacggatgg cgagccggac agacgttccc
      601 atcagcatcg ctgccgcccc tgatggaacg atctggttca ccctggaggg ggcggtcgga
      661 cgattgcgta acggcgcgat cgagaagatt ccgaggagaa tggcggacat cgaaccgctc
      721 gggctcgcgg tcgatgcggg cggggcggta tggttcaccg acacgccgat gcgggcgatc
      781 ggccggctgg atgtcgatgg cgggagtcaa tccttccgct tgcccatgtc aatggtccgg
      841 ctgggccgac tggccgtggc gcccgatggc acggtctggt tcgccgagcc cagcactgtg
      901 agcgtgacgc gcctccggga cggcgtcttc acacgctact tggtcggcac gcccgcgccc
      961 atgggctcga ccaatgtggc accatttggt gtcgccgtcg atccgcacgg gacggtgtgg
     1021 gccacgctgc agaacgccaa ccaactggtc cgcatagcgc cgggtaacca gatgatcacc
     1081 ttcgaggtgc cgacctcgcg cagcggcctc ggggatgttg ccgtcgacgc cgtcggcgcg
     1141 gtctggttct tggagctggc cgcgaataag gtagggagat acgcctcggg acgcttccag
     1201 gagttcaccg ttccaacgcc gagtgccggg ctgaccgccc tggcggtcgc gcccgatggc
     1261 gccgcctggt tcacggagtt gcgcgcccat aaactcggcc gtgtgtaccg ggggaccatc
     1321 acagaattcc cgctgccgcg agaagacgcc cgacccttcg ggctggccgt cgacgcttca
     1381 aacaacgtct ggtacacgga cctatccgga tggctcggcc atcttcctgc cgaccgggct
     1441 cggggttgag actggcccct tcccactggc tcgcgcccac cccgtttgcg atgtattgcc
     1501 gggcggtgag agttgatcag ggggtagtgg tacatcccgg ggagtgtcgg caagcaagcg
     1561 gagacctgcg gtgacctcga gttcgccatg cctggaattg gagcctgacc atatcacggc
     1621 aaaggctgtt ctcctcgccc tagctttgac catctggggt agtccggcgc tcgcccacga
     1681 atcgagcgcg tggggagggc tgtaccgctc acgggatgcc ggagcaacct ggttcctcgt
     1741 cacttctagc cgcaacgtgg tcgcggcgac ggcgctcgcc gtgagtccga tggatgccaa
     1801 ccacctcctg ctcggctcga cggacagcgg tttgctcggc tcgcggaacg ggggtcgcga
     1861 ctggacggcc gaagcgccgg gtattctggg agcggtctac gctgtggcgt tcgatgcgga
     1921 tgggcggcgt gtgctggtct ccaccggctc ggccgtcctt cgcagtgacg atggcggcag
     1981 ttggagcagg cttgcggctc cgagcagttc cgccccggcc cgcgccatcg tgcgtggtgg
     2041 cactcccggg cgtgtatatc ttgtcggtca gaggcaactc gctcgaagtg atgactgggg
     2101 gacctcatgg tcgtccacag ccgacgatct gccagacggg tcagtgactg cgttcgccgt
     2161 gtctcccggc cctccagaga ccgtctatgc aatcatcgat ggccagatct gggtgagagc
     2221 cgaggggaca cccccttggc acggtcggac agcagggatg ccggcaatcg tggtggagac
     2281 gctggcgctg gatccgagcg acccggccag ggtctgggcc gcggggacag gccgggtatt
     2341 tcggagcgat gattcgggta agcattggca atccgtagga cgaccccttc cgacggcgaa
     2401 tacggcggtg cacgggattg ccgcgacagg atcgtcggtt gtcgtttcga cggaccgggg
     2461 tctgtaccgc agctccgatg ggggtgaact ttggcaactg gtgaccgaca atgtccccgc
     2521 tcatctcgag gcgggtcccc tggtccgcga tcccgttgac ccggcaaccg tgtatgcagg
     2581 cttcgccgcg acgccctatg ccgagctctg gcggcgagcg accgaaagtc acgccagtct
     2641 ttggcaggtc aattcacaga agctggggac cgccacggtc ctcgtgatct ttctggccct
     2701 cgatgctgtc ttcggcgcgc aatggctcat gcgccgctac cggacgcccc gccagcagca
     2761 ctgagggtcg gtctgctctg gaatgacgca gtttcactcc gttggctgtc gggttgaccc
     2821 gttatgtctt tttcggcctg acggacgtcc atgagtgggg caggagagtc cgatgacgca
     2881 caccctcggc aaggcggtcg ccgtacttgc cttcgtgacc gccgtgtcca tctcggggac
     2941 cgccggagcg cacaatgttc ctggtggcat ggtgatgaac gcattcgtcc agctggaagg
     3001 cacgcgggcg cttcttgtca tacgggtccc tggccacctg cttgcagaaa tgagcattcc
     3061 gaccgacgtg acgggtgcct caggcccgga gcgcggggga gcggccatgt cgcgcgcgct
     3121 gaccgcgatc acggagggca tcaccatccg ggagcgcgga gtgcccctcg ttccgatagg
     3181 agccgtgggg cggctcacgc tttcgtccga tcgttccttc gagaactacg aggaggctgt
     3241 cgcgcacgtt gcgggcccca cccccccgca actggcgacg tccgcggagc tcgccttcct
     3301 ggatgcccac attacatacc aaatcgctga ccccggctcc gattatacga tccaaaccgg
     3361 gatgctgccg cagctcgggg aaagcgtcaa gctcgtggtg cgcttcatcc ggccagacgg
     3421 ctcgatacgg ccgtatgaga tcaccagcct ctccggaccg gtgcccctcg acccgcactg
     3481 gtatcaagcc gcccgggtgt tcgtcacggc gggcttcttt cacatcctcg acggcatcga
     3541 tcatttgctg ttccttctct gcctggtgat cccattcgat aatctctggg ccttgctccc
     3601 gatcatcact tcgttcacgg tggcccattc cgtcacgctc atcgggtccg catacggtgc
     3661 ggtaccgaag ggcgaatggt tcccacccct tgtggagacg ctgatcgcaa tgtccatcgt
     3721 gtacatggcc ttggagaaca tcatcagggt gaacctccga cgacgctggt tgatcacagg
     3781 ggccttcggt ctcgtccatg gcttcggcct ctcctacggc cttgcgcaga cactccagtt
     3841 tgccggctcc caccttctcc tgtccctgct gtcgttcaat gtggggatcg agcttggcca
     3901 gttggcggta ttgctgctca cgcttccggc cctccgtctg ctgctcggac aactgctgcc
     3961 tggacgaccc ggcaggatgg cgctgtccag catggtggca ggcacgggcc tgtattggat
     4021 gttcgaacga gcccaggttc tctggagggt cccatggcca gttccgaatg ctgccgcctc
     4081 ggtcacagcc ggacggtggg tattgctggt attgctgatg gcaggagccg gatgggtcag
     4141 ccggaagtca ctccgccgcc atgcagcggg gccagggcca agcacgaagg aggacatcgc
     4201 ataacccaaa cgacgatggc ccgctgaacg ccgcgaaatc agccagccga tacagcacgt
     4261 tccttttgtc gccacgagag cggatcccga cttgccgaaa gccgagacgg gcttcgcccc
     4321 ctgcaccacc tgccggccag acctccaccc gatttcacac tagccggcct ccggtgtcct
     4381 agggaatgaa actggcaccc aggacagctc acacggggag gagggcgaac atgg
//
LOCUS       DBNGZC010000955         3582 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_276978, whole genome shotgun sequence.
ACCESSION   DBNGZC010000955 DBNGZC010000000
VERSION     DBNGZC010000955.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_276978"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="AC16G0_16485"
     CDS             complement(<1..888)
                     /locus_tag="AC16G0_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR5101254.1"
                     /translation="MRVTRYSQYTGQWWDNLSLEDLLGELGDYLLQSGFEPGSFEDYL
                     DEMESDQTLQQLYQAILAALADQGRIRSEDVQDWMQGKETEATQEFRQLLKSLVERMI
                     QEGYISLPGDDFGRLERDPRGFMGGNGSESSQVRFEMTNRTIDFLGYRTLRDLLGSLG
                     KSSVGRHDTHELSTGVEASDAPKPYEFGDALNLDIPGTLLHAIQREGLKSPLPLEYDD
                     LLVHQSEYQSSCATVLKLDCSHSMILYGEDRFTPAKRVALALAHLIRTQYPGDSLNIV
                     LFHDGAEEVPVGRLARVAVG"
     gene            complement(950..2356)
                     /locus_tag="AC16G0_16490"
     CDS             complement(950..2356)
                     /locus_tag="AC16G0_16490"
                     /EC_number="6.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase ATPase subunit I"
                     /protein_id="HIR5101255.1"
                     /translation="MRQNLVAKLERREVLFPGVIGYEDTVVPQLINAILSRHHIILLG
                     LRGQAKSRILRSLADLLDEEIPVIAGCEINDNPYAPLCRACRERLAESGERTPVAWLP
                     RDLRYVEKLATPDVTIADIIGDVDPIRAARAGLDLSNELTIHYGLLPRANRGIFAVNE
                     LPDLAGKIQVSLFNIMQEGDVQIKGYPVRLKLDVALVFSANPEDYTARGKIITPLKDR
                     IGSEIRTHYPTDVAHGIAITAQEAWLRRHPDGKLLIPDFIQEVVEQVAFAARESKHID
                     KRSGVSQRLPISCLENVVSNAERRAVVSHERTIVPRPGDVYAAIPSITGKLELEYEGE
                     LRGSEVIVRSLIQEAVGRVFRARLGGAPLQETVGWFDAGGAVKIGDTTPAQEALSFLE
                     HVPGLVEASALLGVPEAADPGLVVSACELILEGLHVQQKIDRSEQRGYTGTPKVEKRP
                     PRDEPSTPPAGGRRRHFN"
     gene            2865..>3582
                     /locus_tag="AC16G0_16495"
     CDS             2865..>3582
                     /locus_tag="AC16G0_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009620776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5101256.1"
                     /translation="MSLLRQKPLALFWFARVATTIAFQMQAVAVGWQGYALTGSAFYL
                     GLIGLAQFLPMFLLTLVVGQVADRYDRRLVAGVCQLVESLAAGALAIGSGGGWLNREN
                     LLAIVFVTGAARAFEGPTMQALVPGLVPGPLIPRAVAWVASASQTATIFGPALGGLLY
                     AAGPTTVYTTAAGLFLGASLLVAVIRVQRVSSTREPVSLQSLFAGIAYIRSRPEILGA
                     ISLDLFAVLLGGATALLPIYA"
BASE COUNT          574 a         1181 c         1126 g          701 t
ORIGIN      
        1 ccccaccgcg acgcgggcga ggcggcccac cggaacttcc tcggccccgt cgtggaacag
       61 gacgatgttg agactgtccc cggggtactg cgtccggatg aggtgagcca gggccaaggc
      121 cacccgcttg gccggtgtga accggtcctc gccgtagagg atcatgctgt gactgcagtc
      181 cagcttgagc accgtggcgc acgagctctg atactccgac tggtggacca gcaggtcgtc
      241 atactccaga gggagcggac tcttcagtcc ctcccgctgg atcgcatgca acagggtgcc
      301 ggggatgtcc agattcagcg cgtcgccgaa ctcatagggc ttgggcgcat cggaggcttc
      361 gactcccgtg gaaagttcgt gggtatcgtg gcggccgacg ctgcttttcc ccagcgagcc
      421 caggagatcc cgcagggtgc gatacccgag gaagtcaatc gtccggttgg tcatctcgaa
      481 cctgacctgg ctgctctccg agccgttccc acccatgaac ccgcgcgggt cgcgctcgag
      541 cctcccgaag tcgtcgccgg gcagtgagat gtagccctct tggatcatcc gctctaccag
      601 agacttcagg agctgacgga actcctgggt ggcttccgtc tcttttccct gcatccaatc
      661 ctggacgtcc tctgaccgga tccggccctg atccgccagg gccgccagga tcgcctgata
      721 caactgctgg agtgtctggt ctgactccat ctcatccaga tagtcttcga agctgccggg
      781 ctcgaatccg ctctggagaa gatagtcccc caattccccg agcaggtcct ccaggctcag
      841 attgtcccac cactgtccgg tgtactggct gtaccgcgtt acccgcattt cccttgcccg
      901 cggattgccc ccagctcgtt ccggccatcc gcctcgcgca gctttcgcat tagttgaagt
      961 gtcttcgccg tcctccggct ggcggcgtcg agggctcgtc gcgaggcggc cgtttctcca
     1021 ccttgggcgt gccggtgtag ccacgctgct cgctgcgatc gatcttctgc tggacgtgaa
     1081 ggccctccag gatcaactcg caggccgaca ccaccaggcc cggatcggct gcctccggga
     1141 cgcccaacag ggctgaggcc tcgaccagcc ctggaacgtg ctccaggaag gagagggcct
     1201 cctgcgccgg cgtcgtgtcg ccgatcttca cggccccacc ggcgtcgaac cagcccaccg
     1261 tctcctggag cggtgcgcct cccaggcgcg ccctgaagac gcgtcccacg gcctcctgga
     1321 tcaacgagcg gacgatgact tcgctcccgc gcaattcccc ctcgtactcc agctccagct
     1381 tccccgtgat ggacgggatt gccgcataca cgtcgcccgg ccgggggacg atggtccgct
     1441 cgtgactcac gacggcccgg cgctcggcgt tgctcacgac attctccagg caagagatgg
     1501 ggagccgctg gctgaccccc gagcgtttgt cgatgtgctt gctctcccgg gcagcgaagg
     1561 caacttgctc cacgacctcc tgaatgaaat ccgggatcag cagcttgccg tccggatgac
     1621 ggcggagcca ggcttcctgc gcagtgattg caatgccgtg ggccacgtcg gtcgggtagt
     1681 gggtgcggat ctccgagccg atgcggtcct tcaggggcgt gatgatcttt ccccgggcgg
     1741 tgtagtcttc cgggttggcg gagaagacca gggccacgtc caacttcagg cgcacgggat
     1801 agcctttgat ctgaacgtct ccctcctgca tgatgttgaa caggctcacc tggatcttgc
     1861 cggccaggtc gggcagctcg ttgaccgcga aaatgccgcg gttcgctcgc ggcagcaggc
     1921 cataatggat ggtcagctcg ttggagaggt cgaggcccgc ccgcgctgcc cggatggggt
     1981 caacgtcccc gatgatgtcg gcgatggtga cgtccggcgt ggcgagcttc tcgacgtagc
     2041 ggaggtcccg gggcagccag gcaacggggg tgcgctctcc cgactccgcc aggcgttccc
     2101 ggcaggcccg gcacagcgga gcgtaggggt tgtcgttgat ctcacacccg gcgatcacag
     2161 ggatctcctc atccaggaga tctgcaagac tccgcaggat ccggctcttg gcctggcccc
     2221 gcagtcctaa gaggatgatg tggtgcctgg acaggatggc gttgatgagc tgggggacga
     2281 cggtgtcttc gtatccgatc acgccgggga agagtacctc gcgacgctca agcttcgcca
     2341 cgaggttctg acgcatctcg tcccgcacgc tgcgtgtgcc gatccgttcc tcgttccatt
     2401 cgctttgccg aagatccccc aacgtcctcg gacgattacc cgatttcccc tgcggcatga
     2461 ggtttttcct cccccaaaga cggcgaaagc cctcgaaata tttcgagggc tgtccggtga
     2521 cagaaaagaa gtaagcacgg aggagtctag aaagctaacc ggtgaatgtc aaaacctttc
     2581 caggccgaaa ggaggacggg tgggcgccag tcacggggat cggattgtgc tggaggaaaa
     2641 gcaggtcacc actccttgaa gagtcccgct gcatcctaaa ggaagactcc gcttcgaatg
     2701 gattcacctc tagaccagcg actcggccag gcatgataga cccaatatcc gaaattgact
     2761 tctcctccct cagggtgcta gcttccccag gtttctagga atggccgcca ggctcgagag
     2821 atcagcgcgt catggatccg tcaactcgta ccccagtccc cgttatgtcc ctcctgcgcc
     2881 agaagccgct cgcgttgttc tggtttgcgc gcgtggcgac cacgatcgcc tttcagatgc
     2941 aggcggtcgc cgtcggttgg cagggctatg cgctcaccgg aagcgccttc tatctcggat
     3001 tgatcgggct cgcgcaattc ctgccgatgt tcctcctgac cctggtggta ggccaggtgg
     3061 ccgatcgtta cgatcgccgc ctcgttgccg gtgtgtgcca gctcgtggaa tcgctcgcgg
     3121 ctggagcctt ggcaatcggc agcggcggcg gttggctgaa ccgggagaac ctcctggcta
     3181 tcgtctttgt caccggcgct gcgcgcgcat tcgagggacc gaccatgcag gcgttggtgc
     3241 caggcctggt gcctggcccg ctgatccctc gtgccgtggc ctgggtggcg tcggcgagcc
     3301 agaccgccac cattttcggg ccggccctcg gcggcctgct gtatgcagcc ggcccgacca
     3361 cggtgtatac gacggccgcc ggcctgttcc tcggggcaag cctgctggtt gccgtgatcc
     3421 gcgtgcagcg ggtgtcctcg acgcgcgagc cggtcagcct gcagtcgctg ttcgccggca
     3481 tcgcgtacat ccgcagccga ccggagatcc tgggcgcgat ctctctcgat ctcttcgccg
     3541 tgctgctggg cggggccacg gcgctgctgc cgatctacgc cc
//
LOCUS       DBNGZC010000956         4280 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_963613, whole genome shotgun sequence.
ACCESSION   DBNGZC010000956 DBNGZC010000000
VERSION     DBNGZC010000956.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_963613"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(447..920)
                     /locus_tag="AC16G0_16500"
     CDS             complement(447..920)
                     /locus_tag="AC16G0_16500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR5101257.1"
                     /translation="MRRTDKEITDRAIMQRIIAQCQICRLGLAHGDTPYVLPVSFGYD
                     GEALYFHTAREGRKIEFLTVNPTVCVEFEHDVRLVPHDTSPCKWTFSFQSVIGYGRVQ
                     ELTDATNKVAGLNQVMRHYSGREWPLDGENLEAIRVWKVPLENLTGKQSKDKLDL"
     gene            complement(1357..2139)
                     /gene="iolB"
                     /locus_tag="AC16G0_16505"
     CDS             complement(1357..2139)
                     /gene="iolB"
                     /locus_tag="AC16G0_16505"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HIR5101258.1"
                     /translation="MKQYSPKDGYTALVKPGKDGIEFLELGILRLPAGGFYRNTSQDS
                     EVCLVLLGGLANVTAGEASLTSIGSRANVFAGRATAVHIPPGLKIKVEAVSTLEVALV
                     RTPSDLPGEPRLIGPEKVKVATRGKDTFERQVHDIIDFNFPSKRLLVGETFNAPGKWS
                     SYPPHKHDTMAPPDETRMEEVYFFKINPPQGFGFQRVYSPEKGFDEAFVIRDNSLTKL
                     PFGYHPTAAAPGYALYYLWVLAGEPRNYVLHDDPDHKWVKEA"
     gene            complement(2260..3228)
                     /locus_tag="AC16G0_16510"
     CDS             complement(2260..3228)
                     /locus_tag="AC16G0_16510"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5101259.1"
                     /translation="MEIFPGVHRLPFRHRDRWLYVYLLVGDWLLLIDTGTAAVPEEVI
                     LPALHDLGIDPTSRRFLALNTHADADHAGGNGSLRLACPQALLLCHERDRNNIEDHEC
                     LWRERYTGCLAPHGLAYAPALAQKMRASMGRPIPVDLVLTGGEVIRLRSDWEVRIFST
                     PGHTRGHIAVHDSRHRTAYIGDAALGRGVPAAEGDQLALPPNYDDVDAYAETTRSLRT
                     LDLDLLCTSHYPLFRGEEIPRFLTDTEEWIGRTDEVILAVLRESRAPVGMKALIAAVN
                     RPLGGWPEGPDLGLAMALTAHLERLERLGQILQVLDARPPAWELQA"
     gene            3457..>4280
                     /locus_tag="AC16G0_16515"
     CDS             3457..>4280
                     /locus_tag="AC16G0_16515"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR5101260.1"
                     /translation="MPLENKSLVKGLAVIETLAGNARPLTAVELSQQLKLARPTLYRI
                     LATLLRRGFVIREDGKPYFRSSLKLLDLGHQVLECTDLLGAARPVLRDLATQFRETVH
                     LAVEEEGRMVYLDKVEGSGPFCTNSRPGRRVPMHCTGLGKAVLAHLPADKVRSILTHH
                     GMQPGTPRTILTMADMERELARVRRQGYAIDDVEFEEGVRCVGAPVFDHRGVPIAAIS
                     VSAPVSRMSRTRAQQVGSILRDATAKVSRAMGWRLQVATLRAGGPPARGEVGGRRR"
BASE COUNT          773 a         1317 c         1430 g          760 t
ORIGIN      
        1 ggccagagcc aaaaggtggc ccggacggaa gacggtcacg atgcctgccg tgcgccccat
       61 cgaacggaag cgcacctgca cagcatccac cgtggccagc cgctcattgg agatggccac
      121 cctgctctga gcctgccctg agcctgtcga agggacgatg tgcgtagcgg ccgaggtttc
      181 gtcgcgagtc ttcgacataa cctcattcgc acgctgattc gagcggtacc tcgagtcttg
      241 cctggggccg gcgaccggcg gcttggcttc gtcgtgggct cggacgaacg ctgttcacca
      301 cccagttgtg agcagggaac gggagggggg aatccggaga ctccggcgca tggggaccac
      361 cgaaacgcag aaggagcgct ccgccccagt cggtaatgtg cttcaggacc aacaaatctt
      421 ggggaggggc tagaggggcg ccacgactac aagtcgagtt tgtcctttga ctgcttccct
      481 gtcaggttct ccagcggaac cttccatacc cggatggcct cgaggttctc cccgtccaac
      541 ggccactccc gtccggagta gtgccgcatg acctgattga gtccggcgac cttgttggtc
      601 gcatccgtca gctcctggac acgaccatac ccgatcacgc tctggaaaga gaaggtccac
      661 ttgcaggggc tggtgtcgtg gggaaccagc cggacgtcgt gctcgaactc cacgcagacg
      721 gttggattaa cggtcaggaa ctcgatcttc cgtccctcgc gggccgtgtg aaagtagagc
      781 gcctcgccat cgtacccgaa ggacacggga agcacatagg gggtgtcccc gtgcgccagc
      841 cccaaccggc agatctgaca ctgcgcgatg atgcgctgca tgatcgcccg gtcggtgatt
      901 tccttatcgg tgcgtcgcat cgatgcctcc attcagtacg tcggctacgg ggtcactccg
      961 aggatcggat atgccttgta ctccgggaac tcgtggcatg ggaagtggtc cggacctgcg
     1021 cgcgggcagt ccgcccgaac tcgccgagga gccggaaggt tgcgccgaga tgcacatcac
     1081 cggtgtggaa cggtcggatc attcacaatg cctctccctg gagcgggaag gtcggctacg
     1141 gagattcccc cgcagacccg cgaatcatct gcgattcctc aggatggcgc aaggccttcc
     1201 gccaattggg ggcgaaacgt tcgggtgtgg gaactacacg ggaccggacg tgtctcagtc
     1261 ggaccgggtg aagcagaaca atttcaaagc aatgaggact taaacggcgg ctgtgccgca
     1321 atcctgcgtc acggggtttc ttcggcgaca gaggaattac gcctccttca cccacttgtg
     1381 atcggggtcg tcgtggagga cgtagttccg cggctcgccg gccaggaccc agaggtagta
     1441 gagggcgtag ccgggggccg ccgcggtagg atggtagccg aaggggagct tggtcaggga
     1501 attgtcccgg atgacaaagg cctcgtcgaa acctttctcg gggctgtaga cccgctggaa
     1561 gccgaagccc tgcggggggt taatcttgaa gaagtacacc tcttccatcc gggtctcgtc
     1621 ggggggtgcc atggtgtcat gcttgtgcgg ggggtagctg gaccacttgc ccggcgcgtt
     1681 gaatgtctcg ccgaccagaa gccgcttgga tgggaaattg aaatcgatga tgtcgtggac
     1741 ctgccgctcg aaggtgtcct tgcctcgcgt ggccaccttc accttctccg gaccgatgag
     1801 gcgcggctcg ccgggcagat cgctgggggt ccgcacgagg gccacctcca gggtgctcac
     1861 cgcctccacc ttgatcttca ggccgggcgg gatgtggacc gcggtggccc gcccggcaaa
     1921 tacattggcc cggctgccga tggaggtgag gctggcctcg ccggccgtta cgttggccag
     1981 gcctcccaac agaacgagac agacctcgct gtcttgactg gtgttgcggt agaagccacc
     2041 ggcaggcaat cgcaggatcc ccagttccag gaattctatg ccgtccttcc cgggcttgac
     2101 cagcgccgtg tacccgtcct tcggagaata ctgcttcatc agatgcatcg ttcgcattcc
     2161 tttcccagca aatccattct ttgtgagaat acccctggcg gtggaagaac gtagcaccgt
     2221 tcccggaaga ctgtcaatcg acggactaca tgcgggtcct caggcttgca gttcccaagc
     2281 gggtgggcga gcatcgagga cttgaaggat ctgccccagc cgctccaagc gctccagatg
     2341 ggctgtcagg gccatggcca ggcccaggtc cggcccctcc ggccagccgc ccaggggccg
     2401 gttgacagcg gcgatcaagg ccttcattcc gaccggtgcc cggctctccc ggagtaccgc
     2461 caggatgacc tcatctgtcc ggccgatcca ctcctcggtg tcggtcagga aacgcgggat
     2521 ctcctccccc cgaaagaggg gatagtggga ggtgcagagt aggtccaggt cgagggtcct
     2581 caggctccgg gtggtctcgg catacgcatc cacgtcgtcg taattggggg ggagagccaa
     2641 ctggtcgccc tccgccgccg gaacaccccg acccagggcc gcatcgccga tataggccgt
     2701 ccggtgtcga gaatcgtgga ccgctatgtg gccgcgggtg tggccgggcg tggagaagat
     2761 tcggacttcc cagtcggatc ggaggcggat gacctcgccc cccgtgagaa ccagatccac
     2821 cgggattggc ctccccatgg aggcccgcat cttctgcgcc agggcggggg cataagccag
     2881 gccatgcggc gcgaggcatc cggtgtaccg ctcccgccac aggcactcgt gatcctcgat
     2941 gttgttccgg tcgcgttcgt ggcagaggag cagggcctgc gggcacgcca gacgcaggct
     3001 cccatttccg ccggcatgat cggcatcggc gtgggtgttc agggccagga atcgacggct
     3061 ggttggatcg atgcccaagt cgtggagggc gggaagaatt acctcctccg gcacggcagc
     3121 cgttccggtg tcgatcagga gtagccagtc ccccacgagc aggtacacgt agagccatcg
     3181 gtcgcgatgc cgaaagggaa gacggtggac gccaggaaag atctccatgg attcggttct
     3241 cgctggctgc tcccagacgc agaaggcagc gcagccgaga caagggaacg gacaacttgt
     3301 atcgggaaga aggccgggtg tcaagggaca agccttctcg gggagcggtc gaggggtcgt
     3361 gccgggaatt agacttgaca agcggttcgg gggaatggta gggaaggagt gcggttgcag
     3421 atattggacg ttctgtccgc atagtgagaa acagccatgc cgctggagaa caagtctctg
     3481 gtcaagggcc tggccgtgat cgagaccctg gcagggaatg cccgcccgct caccgccgtc
     3541 gagctcagcc agcagctcaa gctcgcccgc cccacgctgt accggatcct ggccaccctt
     3601 ctccgccgag gatttgtcat ccgcgaggat gggaagccgt acttccgctc gagtctcaag
     3661 ctcctggacc ttggccatca ggtgctggaa tgcacggacc tgctcggggc cgcccgcccg
     3721 gtcctgcgtg atctcgccac gcagtttcgg gagactgtcc acctggcggt cgaggaagag
     3781 ggccgcatgg tctacctgga caaggtcgag gggtccggcc cgttctgcac caattcgcgg
     3841 cccggccggc gggtgccgat gcattgcacc ggcctcggca aggccgtgct ggcgcacctc
     3901 cccgccgaca aggtacgctc gatcctgact caccacggca tgcagcccgg aacgccacgc
     3961 acgattctga cgatggcgga catggagcgg gaactcgccc gtgtccggcg ccaagggtac
     4021 gccatcgatg acgtggaatt cgaggagggt gtgcgttgcg tgggggcgcc agtcttcgat
     4081 catcgcggcg tcccaatcgc agccatcagc gtcagcgccc ctgtctcccg catgtcgcgg
     4141 accagggccc agcaggttgg ctcgatcctg cgggatgcca ccgcgaaggt ttccagggcc
     4201 atggggtggc ggctgcaggt ggcgacgctt cgagccggag gtccaccggc gaggggcgag
     4261 gtcggcggaa ggcgccgcag
//
LOCUS       DBNGZC010000957         2663 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1944755, whole genome shotgun sequence.
ACCESSION   DBNGZC010000957 DBNGZC010000000
VERSION     DBNGZC010000957.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1944755"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="AC16G0_16520"
     CDS             complement(<1..510)
                     /locus_tag="AC16G0_16520"
                     /inference="COORDINATES: protein motif:HMM:NF012593.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HIR5101261.1"
                     /translation="MKRLRQSLALQIFLGMVLGIIVGGIFGKDVQGLKMIGDIFLRLL
                     QMLVVPFVFAVLLTGTASIGNPKDLGRVGIKVLLMYELTSLIAIALSLGVALAIQPGA
                     GLPKPTGVLPKPPVAMDFKDLFMTFFPSNPFDAASRGDMMQLIVFSLFFGVALGMVKD
                     KVPVVLQVLE"
     gene            1068..1505
                     /locus_tag="AC16G0_16525"
     CDS             1068..1505
                     /locus_tag="AC16G0_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101262.1"
                     /translation="MIHYEHRQRGTTILVTFLAGMLLLLGLASVLPASKGLLFTIIGI
                     LGICGFLFSSLTVRITDRALRWEFGFGLLRKEIPLAEIEKAEVTETTFLQGWGIHHTS
                     RGWLYNVSGFQAVVVRLKSGKTFLLGTDEPDRLLAALLNVLSL"
     gene            1538..2506
                     /locus_tag="AC16G0_16530"
     CDS             1538..2506
                     /locus_tag="AC16G0_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005980904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR5101263.1"
                     /translation="MKDLMMARGARTLLDVCVAVKPGETVLIVTDMVKFPIARVIAAA
                     AMERGAETILSVMQPRARAGQEPPRPVAEAMKNSDVIFLPVSYSVTHTHAVKNAAAAG
                     ARIVALTEFTEDMMIHGGLEANFEELKPICLKVAERFGKGTTARITTPAGTDLQMDIA
                     GRPGNALYCIVGPGQFSPVPNVEANVSPIEGTANGRIVVDASIPYLGIGVIEQPIAVE
                     VQAGFITRIEGGRQADTLRRDLESHGDKNSYNIAELGVGLNPRCRLIGLMLEDEGVLG
                     TAHIGIGTNITLGGAVKAPCHYDLLVWNPRIEIDGQVVIDGKEVRA"
BASE COUNT          542 a          771 c          884 g          466 t
ORIGIN      
        1 ctccaggacc tgaaggacca cggggacctt gtccttgacc attccgagag ccacgccaaa
       61 aaagagcgag aagacgatga gctgcatcat gtcgccgcgg ctggcagcat cgaagggatt
      121 cgagggaaag aacgtcatga agaggtcctt gaaatccatg gccacgggag gcttgggcaa
      181 cacaccggtc ggcttcggca ggccggcccc tggctggatc gcgagggcga cccctaagct
      241 cagggcgatg gcgatgaggc tggtgagctc gtacatcagg aggactttga tgccgacccg
      301 gcccaggtcc ttcgggttgc cgatggaggc cgttccggtc aggaggacgg cgaagacgaa
      361 ggggacgacc agcatttgaa ggaggcggag aaagatatca ccgatcatct tcagtccctg
      421 gacatccttg ccgaagatcc ctcccacgat gatgcccagg accattccga ggaagatctg
      481 gagtgccaac gattgccgca gtcgcttcat ggctcagacc ctccgctgca gaagttttgc
      541 tccggcgacc aggagtcaga gcgcaccgtc ggagcgggaa ggaacagtcg aacctcacat
      601 gtgtccccgc gtgtcagcga gttgccttca ccccctttct tcagcacagc atcccgctgg
      661 cccaccgggc gcaaataaca gaacagcccc tccggggaaa atctcccccg aggggctgct
      721 cgtgttgctt gggccgccat atgatgccgg gcaacaaaat catacctggc ctggcctccg
      781 cgatcccccc ccacctggac caccagacgc cggcgtgaag ggacgatatc attgtcgctg
      841 cgggtgtcaa gcaggatttg cggcggtctg tgttgtaaac agatatgggt ggcaccttcg
      901 gcctcctgca ccagacagaa ggtatcagtc agaaaggagg tcacccatgt ctgtttacac
      961 catgtgaaga agtgccatgc ctgttgcgtc caagggcgga cctcagtaat attccacctc
     1021 tcggactgaa gggtgacagc aggcttccgg gactatgagg tcggcggatg atacattatg
     1081 aacacaggca gcgggggacg acgatcctgg tgaccttcct ggcagggatg ctgctgctcc
     1141 tcgggctggc ctcggtcctg ccggcctcga aaggtctcct gttcacgatc atcggaatcc
     1201 tggggatctg cggcttcttg ttctcgtcgc tcacggtccg gataactgat cgggcgctgc
     1261 ggtgggaatt cggcttcggg ctcctgcgaa aggagatccc gctggcggag atcgagaagg
     1321 ccgaggtgac cgagacgact ttcctccagg gctgggggat ccaccacacg tctcggggat
     1381 ggctctacaa tgtatccggt ttccaggccg tcgtggtgag gctgaagagc gggaagacgt
     1441 tcctgctcgg caccgacgag ccggaccggc ttttggccgc ccttttgaat gttctctcct
     1501 tatagccatg gttgcatgcg cggtttggag gatgcccatg aaggatctga tgatggcgcg
     1561 gggggcgaga acgctcctgg atgtctgcgt ggccgtgaaa ccaggcgaga ccgtcctcat
     1621 cgtcaccgat atggtgaaat tccccatcgc ccgggtgatc gcggctgcgg caatggaacg
     1681 gggtgcggaa acgattctca gcgtcatgca gccgcgggct cgggctggcc aggagccgcc
     1741 caggccggtg gccgaggcca tgaagaattc cgacgtgatc tttctgcccg tgagctactc
     1801 tgtcacccac acccacgcgg tgaaaaacgc ggctgcggcc ggggcgcgga tcgtcgccct
     1861 cacggagttc accgaggaca tgatgatcca cggcggcctg gaggcgaact tcgaagagtt
     1921 gaaacccatc tgcctgaagg tggcggaaag gttcgggaaa ggaacaacgg cgcgcatcac
     1981 gacgcctgcc ggcaccgacc tgcaaatgga catcgccggt cgcccgggga acgccctgta
     2041 ctgcatcgtg gggccggggc agttctcgcc ggttcccaac gtggaggcga atgtctcgcc
     2101 gatcgagggg acggcgaacg gccgcatcgt cgtggacgcg agcatcccgt atctgggcat
     2161 cggggtgatc gagcagccga ttgccgtgga ggtccaggct gggttcatca cgcggatcga
     2221 ggggggccgg caggcggaca cgctcagacg ggatctggag agccacggcg acaagaacag
     2281 ctacaacatc gccgagctgg gcgtcggtct gaatcccaga tgccggctga tcgggctcat
     2341 gctggaggac gagggggtcc tgggcaccgc ccacatcggg atcggcacca acatcaccct
     2401 gggaggtgcc gtcaaggccc cctgccacta cgaccttctg gtgtggaacc ccaggatcga
     2461 aatcgacggc caggtggtca ttgacgggaa ggaggttcgc gcgtaggcga gctgaaactc
     2521 aaggggagat ggcgaggcgc gactagcgcc tggacgtgtt ccgtcggtcg aggacctctg
     2581 ccaggatggg caggaggagc aggccaccgg gaagaatgaa cacgatgagg atggggacca
     2641 gtctagcaat gccccacagg tga
//
LOCUS       DBNGZC010000958         2749 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2524096, whole genome shotgun sequence.
ACCESSION   DBNGZC010000958 DBNGZC010000000
VERSION     DBNGZC010000958.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2524096"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="AC16G0_16535"
     CDS             complement(<1..883)
                     /locus_tag="AC16G0_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015072493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5101264.1"
                     /translation="MPETTSDNARERDRWTSDGLYFEYSKAANPIAAGYITPVPLADF
                     PHHLHEEGATRVIPFDLSKELKCPGPATSPALCANFIRVRGGEGITTKPNATSELYHV
                     IRGRGRTRVHGGEIPWEEGDFFVLPAGSEAAHHAEDDAAFYWVHDEPLLTYLGVKATE
                     PRFKPTLYPRDQARAELHKVEEDPDARTKSRVSVLLANKVFDQTRTITHVLWAMFGVL
                     PAGTVQLPHRHESVALDYIIECEPGCYSLIGDGIDDQGNLKDAKRADWEPGSAFVTPP
                     GLWHSHHNESGAPAHLMP"
     gene            complement(1130..>2749)
                     /locus_tag="AC16G0_16540"
     CDS             complement(1130..>2749)
                     /locus_tag="AC16G0_16540"
                     /inference="COORDINATES: protein motif:HMM:NF013460.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR5101265.1"
                     /translation="RSLATSHPEGRRAAPPGKSPALVSVHAGLIGGSAMLNVELGFPL
                     WVRLTHWFNVLFMTLLIRSGIAILAGHPMLYWNQDCRPGSQWLKFSRKRMPKDRLWTS
                     KDEEVGYPSWLALPGGHNLGLGRYWHFAAVHGWIYATAIYVVLMLAMPPQWRRLIPSS
                     WTIIPDAWHDFVAYITFHLPGPGTTANPAHPYNALQKLTYFGVVFILTPLQLLTGVAM
                     SPTIVSRFPWYPKLFGNRQAARSVHFLGLVAFVGFLLIHVLMVVLHGFAQEMNKMVMG
                     HEDAGWWGAGVGLAILAAVALVHVAGNLVSEAAPRLAHRLLSPLVDPPRRTLLHRAIS
                     VPDYPESEISPYFRVNGYPPTAEYAAEQGGDETYERLRQGNFADYRLEVTGLVEIPLS
                     LSLNDLRAMSRREQITMHHCIQGWTAVGKWAGVRLSDVLDRCQPLPGARYLVFHSYQF
                     HKESGARYYECIDFKMARLPQTILAYEMNDVSLPVAHGAPLRLRVEPKLGFKMVKYLR
                     AIEVVADYRVVGEGMGGVREDHQQYDMGAEI"
BASE COUNT          444 a          877 c          985 g          443 t
ORIGIN      
        1 tgggcatcag gtgggccggc gcacccgact cgttgtggtg cgagtgccag aggccggggg
       61 gcgtgacgaa tgccgagccc ggctcccagt cggcgcgctt cgcatccttg aggttgccct
      121 ggtcgtcgat cccgtcgccg atcaggctgt agcagccggg ctcgcactcg atgatgtaat
      181 ccagggccac ggactcgtga cggtggggga gctgcacggt gcccgcgggg aggaccccga
      241 acatggccca gagcacgtgg gtgatcgtcc gggtctggtc gaagaccttg ttggcgagca
      301 gcacgctcac gcggcttttc gttcgggcgt cggggtcctc ctcgaccttg tggagttcgg
      361 cgcgggcctg gtccctcggg tagagtgtcg gtttgaagcg gggttcggtg gccttgaccc
      421 ccagataggt caggagcggc tcgtcgtgga cccaatagaa ggccgcgtcg tcctcggcgt
      481 ggtgcgccgc ctccgagccg gccggcagga cgaagaagtc cccctcctcc cacgggatct
      541 ctccgccatg cacccgggtc cgaccccggc cgcggatgac gtggtacagc tccgacgtgg
      601 cgttcggttt cgtggtgatc ccttcacccc cgcggacgcg gatgaagttc gcacagagcg
      661 cagggctggt ggccggcccg gggcacttca gttccttgct caggtcgaat gggatgaccc
      721 gtgtcgcccc ctcctcgtgg aggtgatgcg ggaagtcggc gaggggcacc ggggtgatgt
      781 aaccggcggc gatcgggttg gcggccttgc tgtattcgaa gtagagcccg tcactcgtcc
      841 accgatcgcg ttcgcgcgcg ttgtcgctcg ttgtttcagg catgggacct ccttcttggg
      901 ttgctggagg atgggatatc gacggtgcgg gtcattcctg cgccgggtcc ggcggccgcc
      961 cggtcgggac tcgtttcgcc ccggaccagc aggggaatac cgaagcggac agcaagttcc
     1021 agggtaacgc gagcccccgc gactccacga accatgaccc tggcaggaca tgccatcggg
     1081 aagctggccg cctgattcga agcgtctggc cggagcaccg ggcttgaagt cagatctcag
     1141 cccccatgtc gtactgctgg tggtcctcac ggaccccgcc catcccttcg ccgacgacgc
     1201 ggtagtccgc caccacctcg atcgcccgca gatacttgac catcttgaaa ccgagcttgg
     1261 gctcgacccg cagccggagc ggcgccccgt gtgccaccgg caacgacacg tcgttcattt
     1321 cataggcgag gatcgtctgg ggcagacgcg ccatcttgaa gtcgatgcac tcgtagtaac
     1381 gcgccccgct ctccttgtgg aactggtacg agtggaagac gagataacgc gcgcccggca
     1441 gcggctgaca gcggtcgagg acgtcggaga ggcggacccc cgcccatttc cccacggccg
     1501 tccagccctg gatgcagtgg tgcatcgtga tctgctcccg ccgggacatc gcgcgcaggt
     1561 cgttcagcga gagcgagagg gggatctcga ccaggccggt gacttcgagc cggtagtcgg
     1621 cgaagttccc ctgccggagc cgctcgtagg tctcgtcgcc gccctgctcc gccgcatact
     1681 cggcggtcgg ggggtagccg ttgacgcgga agtagggaga gatctccgac tcggggtagt
     1741 cggggaccga gatcgcgcgg tgcagcagcg tgcggcgggg cgggtcgacc aggggcgaaa
     1801 gcaggcggtg ggcgagccgg ggggccgcct ccgaaacgag gttgcccgcc acgtgcacga
     1861 gggccacggc ggcgaggata gccagcccga ccccggcccc ccaccatccg gcgtcctcgt
     1921 gccccataac catcttgttc atctcctggg cgaacccgtg caggaccacc atgagcacgt
     1981 ggatcaggag gaacccgacg aaggccacca ggccgaggaa gtggacgctc cgcgccgcct
     2041 ggcggttgcc gaagagcttg ggataccagg ggaagcgcga cacgatcgtc ggcgacatcg
     2101 cgacgccggt caggagctgg agcggcgtca ggatgaagac cacgccgaag taggtgagct
     2161 tctggagggc gttgtagggg tgggccgggt tggccgtggt gccggggccc ggcaggtgga
     2221 aggtgatgta ggccacgaag tcgtgccagg cgtcggggat gatcgtccag gacgacggga
     2281 tgagccgccg ccactggggg ggcatcgcca gcatcaacac gacgtagatc gcggtggcgt
     2341 agatccagcc gtgcacggcg gcgaagtgcc agtaacgccc caggccgagg ttatgcccgc
     2401 ccggcagggc cagccacgac gggtagccga cctcctcgtc cttggaggtc cagagccggt
     2461 ccttcggcat ccgcttccgc gagaacttca accactggct cccgggccgg cagtcctggt
     2521 tccagtagag catcgggtgc ccggccagga tcgcgatgcc gctgcggatc agcaaggtca
     2581 taaacaggac gttgaaccag tgcgtcaggc gcacccagag gggaaagccc agctcgacgt
     2641 tcagcatggc cgatcctccg atgagcccgg cgtggacgga gaccagggcc ggagacttgc
     2701 cgggcggcgc ggcccgccgg ccttccgggt gagatgtcgc caggcttcg
//
LOCUS       DBNGZC010000959         5188 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_180378, whole genome shotgun sequence.
ACCESSION   DBNGZC010000959 DBNGZC010000000
VERSION     DBNGZC010000959.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5188)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_180378"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="AC16G0_16545"
     CDS             <1..705
                     /locus_tag="AC16G0_16545"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIR5101266.1"
                     /translation="NLPVLMGLLAIWNNNFLGAETIAVLPYEQYLKRFPAYLQQLTME
                     SNGKHVTLDGKPVAHDTGPIYWGEPGTNGQHSFYQLIHQGTRLIPCDFIAFAQPLHPL
                     GRHHDLLLANVFAQTEALAFGKTAEAVKAEGTPDWLVPHRVFEGNRPSTTILAERLTP
                     ETLGKLVALYEHAVFAQGTLWQINSFDQWGVELGKVLAQRIIPELESQAEPTLGHDSS
                     TNTLIRRYRRLKEAVR"
     gene            784..1686
                     /locus_tag="AC16G0_16550"
     CDS             784..1686
                     /locus_tag="AC16G0_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010873024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HIR5101267.1"
                     /translation="MITMSNYTEQLQKMKTHPGFIAALDQSGGSTPHALRSYGIKEEA
                     WSNEAEMFTIVHQMRTRIITSPSFTGERILGAILFENTMDRDIAGQPSADYLWNVKRV
                     VPFLKVDKGLAAEKDGVHLMKPMPELATLLAKAKAKRIFGTKMRSVINQASAAGIKEI
                     VNQQFDVARQIIAAGLVPIVEPEVDIHCPEKAKAEALLKAALLEKLNDLPGGQLVMLK
                     LTLPEQDDFYAECVTHPRVVRVVALSGGYTREEANSRLRRNHGIVASFSRALVEGLTA
                     QQSDAEYNALLGASIQSIYDASNT"
     gene            1780..2457
                     /gene="rpe"
                     /locus_tag="AC16G0_16555"
     CDS             1780..2457
                     /gene="rpe"
                     /locus_tag="AC16G0_16555"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR5101268.1"
                     /translation="MDRGSVKIAPSILAADFARLGEQVIEAERAGADRIHVDVMDGHF
                     VPNLSMGAPIVQALRRVTKLPLEAHLMISDPDAFLDQFAAAGSDSILVHWEGNNNLHR
                     TVQRIRALGKRPGVAINPATPVVVLDEIVRIVDQVLVMTVDPGFGHQQFLPTTLPKIR
                     RVRQMIEQGKLGCDVEVEGGIDSETASLVTEAGANVLVAGTAIFGDRQGVAAGMNRLR
                     AVADLQR"
     gene            2532..3023
                     /locus_tag="AC16G0_16560"
     CDS             2532..3023
                     /locus_tag="AC16G0_16560"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HIR5101269.1"
                     /translation="MCVGIATDHGGFGLKEELLAQLRAAGHEVVDFGAHGLNPGDDYP
                     DFVIPLAQAVAAGKVERGVAICGSGVGASVCANKIPGVRAGLVHDHFSARQGVEDDDM
                     NIICMGGRTVGPSVAWDLVETFLAAGFSQAERHLRRLGKVGSLEPEVISDRRGAGRAP
                     HRT"
     gene            3131..>5188
                     /gene="tkt"
                     /locus_tag="AC16G0_16565"
     CDS             3131..>5188
                     /gene="tkt"
                     /locus_tag="AC16G0_16565"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR5101270.1"
                     /translation="MSTTQVAQPMTGVEADQLCINTIRTLSIDAVQAANSGHPGTPMA
                     LAPLVYTIWNRIMRFDPQDPIWPNRDRFVLSNGHASMLLWSVLHLTGTRAVNADYERL
                     GHPSVSLDDIRRFRQLDCKAPGHPEYHWVSGVETTTGPLGQGVATSVGMAIAQKWLAA
                     RYNRPGFTIFDYNIYAVCGDGCMMEGIASEAASLAAHLGLDNLCWVYDNNHITIEGNT
                     RITFTEDVATRFLGYGWNVLRVGDANDIDRIQRALEVFGKTKGRPTFIILDSHIGYGS
                     PHKQDTAAAHGEPLGEEEVRLAKRSYGWPEDAKFRVPDGVYEHFAAGVGARGGQARRQ
                     WTELFAGYRVKYPELATEIDLMQRRELPVGWDKNLPVFPADPKGLAGRDASGKVLNVL
                     AQNIPWFLGGSADLGPSNKTTLTYTGAGDFQADSPGGKNLHFGIREHAMAAIVNGLSL
                     SKLRAFGASFFIFSDYARPAIRLSALMELPTIFIFTHDAMGDGEDGPTHQPVEQLLSL
                     RAIPGLTMLRPGDANEVVEAYRCVMQLRHRPAVLTLSRQPLPTLDRKKYAPASGVASG
                     AYVLADAPGGNPEVILIATGSELSLVAEAHEKLLAEGIRSRVVSMPSWDIFDRQSREY
                     RDSVLPPTVKARVAVEQGSILGWERYVGESGRIIGMHTFGASAPLKELQRKFGFEPDR
                     VVAA"
BASE COUNT         1011 a         1598 c         1678 g          901 t
ORIGIN      
        1 aaccttccgg tgctcatggg cctcctggcg atctggaaca acaacttcct gggcgcagag
       61 actattgccg tcctgcccta tgagcagtac ctgaaacggt tcccggccta cctgcagcag
      121 ttgacgatgg agagcaacgg gaagcacgtc acgctcgacg ggaagccggt cgcccacgac
      181 accggtccca tctactgggg cgagcctggg accaacggcc agcactcctt ctaccagttg
      241 atccatcaag ggacccggct catcccctgc gacttcatcg ccttcgccca gccgctgcac
      301 cccctcggcc ggcatcacga cctgctcctg gcgaacgtct tcgcccagac cgaggcgctg
      361 gctttcggca agacagccga agcggtcaag gccgagggga cgccggactg gctcgtcccc
      421 caccgggtct tcgagggcaa tcggccgtca accacgattc tcgccgagcg gcttacacca
      481 gagaccctcg ggaagctcgt ggccctctac gagcacgcgg tcttcgccca ggggaccctc
      541 tggcagatca actccttcga ccagtggggg gtcgagctgg gcaaggtcct ggcccagcgt
      601 atcatcccgg aactcgaaag ccaggcagag cccacgctcg gccatgacag ctccaccaat
      661 accctgatcc gccgctatcg gaggctcaag gaggcagtgc gatgactctc ggcttggaca
      721 agccgctgtt cgtgttgcct ttcgaccgca cgtcggtccg ccccccaacc ccaggaatct
      781 gaggtgatca cgatgagcaa ctatacagag caactgcaga aaatgaagac ccatcccggt
      841 ttcattgcgg ccctggatca aagcggtggc agcacgcccc acgccctgcg gtcttacgga
      901 atcaaggaag aggcgtggtc caacgaggct gagatgttta cgatcgtgca tcagatgcgg
      961 acgcgcatca ttaccagccc cagcttcacc ggtgagcgga tcctgggcgc catcctgttc
     1021 gagaacacca tggaccggga tatcgcgggg cagccgagcg ctgattacct atggaatgtg
     1081 aaacgggtgg tgccgtttct caaagtggac aagggccttg ccgcggagaa ggacggcgtg
     1141 cacttgatga agccgatgcc cgagcttgca acgctgctcg ccaaggccaa agccaagcgc
     1201 atcttcggga ccaagatgcg ttcggtcatc aaccaagcca gcgcagcggg catcaaggag
     1261 atcgtgaacc agcagttcga cgtggctcgg cagattatcg ccgcgggcct cgtgccgatc
     1321 gtcgagccgg aagtggacat tcactgcccg gaaaaagcca aggccgaggc tttgctcaag
     1381 gcggccctcc tcgagaagct caacgatctg ccaggggggc aactggtcat gctcaagctc
     1441 accctgccgg agcaagacga cttctacgcc gagtgtgtca cccatcccag agtcgtgagg
     1501 gtggtggcgc tgtcgggcgg gtatacgcgg gaagaagcaa acagtcgcct gcgcaggaat
     1561 cacggcatcg tggcgagctt ttcgcgggcg ctggtcgagg ggctgacggc ccagcaatcc
     1621 gatgccgagt acaacgccct cctgggtgca tccattcaga gcatctatga cgcgtccaat
     1681 acctgagcgg gcgatgcccg aagaatcgga gaggaatcgg atgagggggg tggcggcgca
     1741 cgaacggtcc tagacctgtg accacgaggg acttgggata tggacagagg ttctgtgaaa
     1801 attgccccgt caattcttgc ggccgacttc gcccggttgg gcgagcaggt gatcgaagcc
     1861 gagcgggccg gggcggaccg gatccacgtc gatgtgatgg atggccactt cgtgcccaac
     1921 ctctccatgg gggcgccgat cgtccaggcg ctgcggcggg tgacgaagct accgctcgag
     1981 gcgcacctaa tgatctcgga cccggacgcc ttcctcgacc agtttgctgc ggccggttcc
     2041 gactcgatcc tcgtccactg ggaaggcaat aacaacctgc accggaccgt gcagcgaatc
     2101 agagcgttag gcaagcgccc aggggtggcc atcaacccgg caacgccagt ggtggtgctg
     2161 gacgaaatcg tccgcatcgt ggaccaggtg ctggtcatga cggtggaccc gggtttcggc
     2221 caccaacaat tcctcccgac gaccctgcca aagatccggc gtgtccgtca gatgatcgag
     2281 cagggtaagc tcgggtgtga cgtggaggtg gaggggggca tcgactcgga aacagcgtcc
     2341 ctggtaaccg aggccggagc caatgttcta gtggcgggaa cggcgatttt cggcgatagg
     2401 caaggggtgg ccgcggggat gaatcggttg cgagccgtcg ccgacctgca acggtagaat
     2461 ttgtccgacg gcgcaagtgc cggggattac gcgaagagtc agaaccaaag aaataaccac
     2521 aaggagaaga tatgtgcgtg ggaattgcca cggatcacgg tgggtttggg ttgaaggagg
     2581 aactgttggc ccagctccgc gcggcggggc atgaggtagt cgatttcggg gcgcatggcc
     2641 tgaatcctgg cgacgactat cctgattttg tcatcccgct cgctcaggcc gtggcggccg
     2701 gaaaggtgga gcgcggcgtg gcaatctgcg gcagcggcgt cggggcctcg gtttgtgcta
     2761 acaagattcc gggtgtccgc gccgggctag tgcacgacca tttctcggct cggcaagggg
     2821 tcgaggacga tgacatgaac attatatgta tgggcggccg gaccgtaggt ccatccgtgg
     2881 cctgggacct cgtcgagacg ttcctggcgg cgggcttcag tcaagccgag cgccacctgc
     2941 gtcgcctggg caaagtgggt tctttggagc cggaagtgat cagcgaccga cgaggggccg
     3001 gaagggcacc gcacaggacg tagattcata cactcgcggt cggtggcgcc cctgaccgcc
     3061 aaagcacggc gacccgccgg ccgtggtgcg gatgtcggga agaaaatcag ggtatctcaa
     3121 ggagaaaacc atgagtacca cgcaagttgc tcaaccaatg acaggtgtag aagccgatca
     3181 gttgtgtatc aataccatcc gtacactgtc gatcgatgcg gtacaggcgg ccaattccgg
     3241 acaccccggg accccgatgg cgctcgcacc gctggtctac acaatctgga atcgcatcat
     3301 gcgtttcgat ccacaggacc cgatctggcc gaaccgcgat cggttcgtgc tatcgaacgg
     3361 gcacgcctcg atgctgctct ggtcggtgct ccacctcacc gggacgcggg cggtgaacgc
     3421 cgactatgag cggctggggc acccttcggt gagcctcgac gacatccgcc gctttcgcca
     3481 gctcgactgc aaagcccctg gtcaccctga gtaccactgg gtctcgggtg tggagacgac
     3541 gaccggcccg ctgggacagg gcgtcgccac gagcgtgggg atggccatcg ctcagaagtg
     3601 gctcgccgcc cggtataacc ggcctgggtt tacgatcttc gactacaaca tttatgcggt
     3661 ctgcggagat ggctgcatga tggagggcat tgcgtcggaa gcagcgtcgc tggccgctca
     3721 tctgggcctc gacaatctct gttgggtcta tgacaacaat catatcacca tcgaaggcaa
     3781 cacgcgcatc acctttacag aggacgttgc aacccggttc ttgggttatg gatggaatgt
     3841 tctgcgggtg ggcgacgcca acgacatcga tcgcattcag agggctctcg aggtgttcgg
     3901 caagacgaag ggccggccca ccttcatcat tctcgacagc catatcggct acggttcacc
     3961 gcacaagcag gacaccgcag cggcgcacgg cgagccgctg ggcgaggagg aggtccgtct
     4021 tgccaaacgc agctacggat ggcccgagga cgcgaagttc cgcgtgcccg acggcgtcta
     4081 cgagcacttc gcggctgggg tgggcgcacg cggtgggcag gctcgacgcc agtggacgga
     4141 gctcttcgct gggtacaggg tcaagtaccc cgagctcgcg actgagatcg acctgatgca
     4201 gcgacgcgag ctgccggttg gctgggacaa gaacctccct gtcttcccgg ccgacccgaa
     4261 agggctggcc gggcgggacg cctcgggcaa ggtgctgaac gtgctggccc agaacatccc
     4321 ctggttcctg gggggctcgg cggatctcgg gccgtcgaac aagaccaccc tgacctacac
     4381 gggcgcggga gatttccagg ccgacagtcc gggtgggaag aacctgcatt tcggcatccg
     4441 cgagcacgcc atggcggcaa tcgtcaacgg gctctcgctg tccaagctcc gggccttcgg
     4501 tgccagcttc tttatcttca gcgactacgc gcggcccgcc atccggctct ccgcgttgat
     4561 ggagctgccc accatcttca tcttcacgca tgatgccatg ggtgacggcg aggacgggcc
     4621 gacccaccag cccgtggagc agttgctctc gttgcgcgcc atcccagggc tcaccatgct
     4681 ccggccgggg gacgccaacg aggtggtcga ggcctaccgc tgcgtcatgc aactgcgcca
     4741 ccggccggcc gtgctgacgc tctcccgcca gcctctgccg actctagacc ggaaaaagta
     4801 tgccccggca tccggcgtcg cgagcggtgc ctacgtgctc gccgacgccc ccggcgggaa
     4861 tcccgaggta atcctcatcg ctactggcag cgaactcagt cttgttgctg aggcccacga
     4921 gaagctgctc gccgagggca ttcgctcgcg cgtggtctcc atgccgtcct gggatatctt
     4981 cgaccgccag tcgcgggagt accgagatag cgtactgccg cccaccgtca aggcgcgggt
     5041 cgccgttgag cagggctcta tccttggatg ggagcgatat gtgggcgagt ccggccggat
     5101 catcggcatg cacacgttcg gagcctcggc gccgctcaaa gaactccaga gaaaatttgg
     5161 tttcgagccg gaccgggtgg tggcagcc
//
LOCUS       DBNGZC010000960         2149 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2233891, whole genome shotgun sequence.
ACCESSION   DBNGZC010000960 DBNGZC010000000
VERSION     DBNGZC010000960.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2233891"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="AC16G0_16570"
     CDS             <1..421
                     /locus_tag="AC16G0_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR5101271.1"
                     /translation="EDVAAGHLLAAERGKVGERYILGNRNLALCEIFECLGRIAGIRP
                     PRLKLSAGAILPVARLSEWIADHLTGRPPLIAVDAVQMARKTMFFDCQKAIRELGLPQ
                     SPVEEALAKAVRWFRANGYAPVRQGDGLTSHGGRAGR"
     gene            418..1239
                     /locus_tag="AC16G0_16575"
     CDS             418..1239
                     /locus_tag="AC16G0_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HIR5101272.1"
                     /translation="MSDLPRLLQGSLLLRPYVFMFLLLHLVGAAALLGWRRTGAFTLF
                     TWGVAFAAEASSTRTGIPFGWYYYIPRTADRELWILGVPFFDSLSFPFLLVASYALAW
                     LLLTGGRPLHPDLAGIGERKRERPGRGPHLALTTFLFVLIDVVIDPVALRGDRWFLGQ
                     IYGYPEPGMYFGVPLANFLGWGVVGLVSMGLYQTWELRQPDSGTAAFRGRALLCPGLY
                     FIVLAFNLTVTFAIGEWVLGLCGVALSLPLLLWTWRMLRGPRVLGWRPRGEGIRA"
     gene            1236..>2149
                     /gene="hpnH"
                     /locus_tag="AC16G0_16580"
     CDS             1236..>2149
                     /gene="hpnH"
                     /locus_tag="AC16G0_16580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HIR5101273.1"
                     /translation="MRFPIQLHWTLTKYILGNALRGRQRYPFVLMLEPTHLCNLECEG
                     CGKIREFENTIRDMLPLETCLKAVEECGAPIVSICGGEPTIYPHIGELVSELLRRKKF
                     VYLCTNAIKLDKWLPKWTPSPYLTINVSMDGLEPTHDLVRSRKGLYQIDIRMIKLAKA
                     QGFRVVTNTTIYKETKIEELEQLFAELEAIGVDGFLVSPAYEYTVLKGRDIFLETRQT
                     HEKFQRVWELAKRYRLFSTPMYLRYLKGERELKCSPWGNPNYNPQGWKGPCYLITDAH
                     YRTFNDLMGKTDWEKYENKRDERCNNCM"
BASE COUNT          369 a          658 c          714 g          408 t
ORIGIN      
        1 ggaggacgtg gcggcggggc atctgctcgc ggccgaacgg gggaaagtgg gggaacggta
       61 catcctcggg aaccgcaacc tggccttgtg cgagatcttc gagtgtctgg ggcggatcgc
      121 gggcatccgg ccgccccgtc tgaagctctc cgctggcgcc atcttgccgg tggccagact
      181 ttcggagtgg atcgccgacc acctgacggg gcgaccaccc ctgatcgcgg tggacgcggt
      241 gcagatggcg cggaagacga tgttcttcga ctgccagaag gcgatccggg aactgggatt
      301 gccgcaatcg ccggtggaag aggccttggc caaggcggtg cggtggtttc gggccaatgg
      361 ctatgcgccg gtccgacagg gggatgggtt gacgtcccac gggggacgtg cggggagatg
      421 agcgacctgc cccggcttct gcagggaagc ctgctcctcc gcccttacgt tttcatgttc
      481 ctgctgcttc acctcgtggg ggcggctgcg ctgctgggct ggcggcgcac cggggccttc
      541 accctcttca cctggggcgt ggcctttgcg gcagaggcca gctcgacccg gaccggcatc
      601 cccttcgggt ggtattatta catcccccgg acagccgacc gggaattatg gatcctgggg
      661 gtgccgttct tcgattcctt gtcgttcccc tttctcttgg tggcaagcta cgctctggca
      721 tggctgctgt tgacgggtgg ccgaccgctt cacccagatc tcgcggggat cggggagcga
      781 aagcgggaac gtccggggcg gggtccgcac ctcgctctga ccacgttcct cttcgtcttg
      841 atcgacgtgg tgatcgaccc ggtggccctg cggggtgacc ggtggttcct gggccagatc
      901 tacggctacc ctgagccggg aatgtatttc ggggtgcccc tggccaattt ccttggctgg
      961 ggtgtggtgg gcctggtatc catgggcctg taccagacct gggaacttcg tcagccggac
     1021 tcggggaccg cggcatttcg gggccgggcg ctcttgtgcc cgggactcta tttcatcgtc
     1081 ctggccttca acctgacggt caccttcgcc atcggggagt gggtgctggg actctgcggt
     1141 gtggcactca gcctgccgct gttgctctgg acctggcgta tgctgagggg accgcgggtc
     1201 ctcgggtgga ggccgcgggg ggagggaata cgggcatgag gtttcccata cagctccact
     1261 ggaccctgac aaaatacatc cttggcaacg ccctgcgcgg gaggcagcgc tacccgttcg
     1321 tcctgatgct ggagcccacc cacctgtgca acctggagtg cgaaggctgc ggcaagattc
     1381 gtgaattcga gaacaccatc cgggacatgc tgcccctgga gacctgcctg aaggccgtgg
     1441 aggaatgcgg ggcgcccatc gtgtcgatct gcggtggcga gccgacgatc tatccccaca
     1501 tcggagagct ggtctccgag ttgctccggc gcaagaagtt tgtctatctc tgcaccaacg
     1561 ccatcaagct ggacaagtgg ctccccaagt ggacgcccag tccctacttg accatcaacg
     1621 tctccatgga cggcctggag ccgacccacg acctggtccg ctcccgcaaa gggctctacc
     1681 agatcgacat ccggatgatc aagcttgcca aggcccaggg attccgggtc gtgaccaaca
     1741 ccacgatcta caaggagacg aagatcgagg aactcgagca gctcttcgcc gagctggagg
     1801 ccatcggcgt ggacggcttc ctggtctctc cggcgtacga gtatacggtg ctgaagggcc
     1861 gcgacatctt cctggagaca cggcagacgc acgagaagtt ccagcgcgtc tgggagctgg
     1921 ccaagcgcta ccgtctcttc agcaccccca tgtacctgcg ctacctgaaa ggggagcgtg
     1981 agctgaagtg ctccccctgg ggaaacccga actacaatcc ccaaggctgg aaggggccgt
     2041 gctacctcat cacggatgcc cactacagaa ccttcaacga cctgatgggc aagaccgact
     2101 gggaaaagta cgagaacaag cgggacgagc gctgcaacaa ctgcatgat
//
LOCUS       DBNGZC010000961         7674 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_473216, whole genome shotgun sequence.
ACCESSION   DBNGZC010000961 DBNGZC010000000
VERSION     DBNGZC010000961.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7674)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7674)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7674
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_473216"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="AC16G0_16585"
     CDS             complement(<1..391)
                     /locus_tag="AC16G0_16585"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR5101274.1"
                     /translation="MPTVPKTMRAAVIRAWNEVHIEEVPVPRFGPKEVLLRVRACGIC
                     PTDLRIVSGAYYPHWPPSLPFIYGHEWVAEVAAVGEAVTQFKVGDHVTGENHIGCREC
                     RMCRVGRYNLCFRADDGSQVLYGHNAPG"
     gene            complement(489..1247)
                     /locus_tag="AC16G0_16590"
     CDS             complement(489..1247)
                     /locus_tag="AC16G0_16590"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR5101275.1"
                     /translation="MAWGNSLFEISAAEADKIYSKTNIAVIPTGSTEQHGPHLPMGTD
                     AMAGIAFGKRVAEKLKAVFVPFSVLGVTPYHMPWKGTISLKSSTFMALFTDVVDCLYQ
                     HGIRRVLVSNGHEGNIAPLRVAADDLQQKYRDLRIVIADVWVVVNNLFPDLERTHAAE
                     MEAMEVLCYYPALFHPELGTNPSDLQEGRVGHERFRRKDVYTMFRDFREVAETGWYGS
                     LERAKVERIPQVLEKVSDVIAKYAVEMFDAVQKY"
     gene            complement(1270..2313)
                     /locus_tag="AC16G0_16595"
     CDS             complement(1270..2313)
                     /locus_tag="AC16G0_16595"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR5101276.1"
                     /translation="MTGRMFLRLGVLAALVGLLLAPVAWAAAPAPSSVINLKFSHHEP
                     PTGRTGMAFQKWADMVGEKTNGKVKIQVFAAQTLGKGPDTYAMVQNGIADIGWVMVGF
                     YAGQFPLSEVYNLPMLGVQSGKAGASALWDYYQGSPAVQKEWSNVKVLTTFSSGVQFV
                     NTAKKPVRTLQDIKGQKIRVTAWGATNFIKNMGGNPIAMSPPEMYDGISKGVIDGMVF
                     DWQGIQSSRLYEVLSYATYAPLVQVPQFLVMNPDRWKSLPPDVQKVFEEMGGRFGAQM
                     LGGAFDEADPIGVENFKKLGKEVIMLPADEQAKWQAAAKPVWAAWVDQMKSKGLDGQA
                     ALDQIQKAIAKNK"
     gene            complement(2494..3036)
                     /locus_tag="AC16G0_16600"
     CDS             complement(2494..3036)
                     /locus_tag="AC16G0_16600"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HIR5101277.1"
                     /translation="MGWILTFRRWVRLLCVTLATVGALALMAMMFLIAFDVIGRDVLD
                     SAILGTFEVVEYLMVPTVFLALAYAQLEDTHINVDVAIQFLSRRTRAALSVVTLLLTL
                     GVFLPMTWVGYREAMQVYVQRQVSTVLLIPRWPFQVLMVIGLVAFCLAIVADMLVAIA
                     RASGMEIDPKEHSGEKLVAD"
     gene            complement(3057..4358)
                     /locus_tag="AC16G0_16605"
     CDS             complement(3057..4358)
                     /locus_tag="AC16G0_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006422083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR5101278.1"
                     /translation="MTPIEIGILGTAFMVALLFSGISVGVAMALTGFLGFAVLSGITP
                     ALGLLKTVPYSTVASYTLTVIPLFILMGEMSFQSGISRDLYDGAHRWFGHLPGGLAMA
                     TIGAGAGFGAMCGSSAASTATFGSVALPEMRKYKYAPGFAAASVAAAGTLAILIPPST
                     GFIIYGVITEQSIGKLFIAGIIPGIILSALYILTIYFIAKRNPEIAPRGPATTWREKF
                     GVLRGSWPMVVLFLFVMGGIWVGIFTATEAGAMGAFGSFLFMIWRRQCNFRNMVDCLA
                     KTAKITAMVFLILIGAFIFGYFLAVTGVPEAMATYFRSLPLPPIMIILGILILYFFLG
                     CIMDMLSMVVLTTPLFFPIVVGLGFDPIWYGVMVVVAMEQGQLTPPVGLNLYVISGMA
                     RDIPMSQIIYYMWPYILDLVIFMFIMLAFPKLALFLPGLMK"
     gene            complement(4515..5108)
                     /locus_tag="AC16G0_16610"
     CDS             complement(4515..5108)
                     /locus_tag="AC16G0_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5101279.1"
                     /translation="MVIDLRKCVGCNACTVACKQEHGTPPGIHWSKVMQYEVGTYPKV
                     RLRFLPMLCMHCQDAPCLEACPAGATYRRQDGPVLVEAAKCIGCRYCIMACPYEARSY
                     NDGEPQEYFAGKGLTRVEEAGSQQHTAGVVEKCTFCVHRLDQGKEPACVATCPAEARI
                     IGDLDDKDSEVARLVTSGLAKPRLEELGTHPLVFFIE"
     gene            complement(5117..>7674)
                     /locus_tag="AC16G0_16615"
     CDS             complement(5117..>7674)
                     /locus_tag="AC16G0_16615"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR5101280.1"
                     /translation="SPREEWKVTGCRMCGNKAWCTARVKVRDGVVVKIEGDHTNPVSQ
                     GKLCSRGQAALFNLYNPYRVKAPMKRTNPKKGLTQDPRWVEISWEEALSTVADRLKAI
                     RRDDPRKLAHVWGFGGYWWVVTDDAFLPAFGTPNKLRTHGVLCPVHYGCALVQGTLLD
                     KQDVEYCQYLITIGGTLGPNIGSSHSIRALARARERGMRIVVVDPRCSHEASLADEWV
                     PIRPGTDLAFSLAMLYVLLHEIKVFDEPFLKRRTNAVYLIGDDGYYLKDGATGKPMIW
                     DPVDGRPKVFDDPTIKDYALAGRYPVGDTAGTVAFQLVKDHVKPYTPEWAEPITTVPA
                     ATVRRLSWEFVEAAHIGETIELDGYAFPFRPAIVKAERGTLGKKGGAYQHLVSKMLCM
                     LVGSLDVPGGYLGSATGPVLSPGPDGVVAPKSEATPIPFKFPPDIDLRQYYPNRHTMP
                     HMAWKAILDPVKYGVPYPIDTLMIYGGNPVTNNANPEEAVAALAKVPFVVTIAYLYDE
                     AAEFSDILLPESALLERYGIQKLGDHLVQAVDEPGLRVTGVLARTPVVERQYDTMQAD
                     DIYIELAERVGFLYGEGGLNDQLNRFYELKAPHALELDKRYGTPEIIERILKGKYGVE
                     NGLQAFTSSAVQYQMRSRKESYNYYYYPMGTTRHPFYFEHVLRTGDTLRANLERHGTA
                     IPGQDMEDVWRFYRPIPSWVGRPDENAPPDYDLWAMNWATPQFRMNCGDQTGNPLLNE
                     VVDRCDPYQFTVLLNKSTAQRKGLADGERVVVEAYWGGRTEGILRVTDLMHPDAVGIP
                     AIHGFRSMHRNPITRRGPHFNSLLSSAEGSFDPLHGGIDRSPRVKVYRAGASR"
BASE COUNT         1393 a         2507 c         2362 g         1412 t
ORIGIN      
        1 cccccggcgc gttatggccg tacagcacct gggagccgtc gtcggcccgg aaacataggt
       61 tgtagcgacc gacgcggcac atgcgacact cgcggcagcc gatgtggttc tcgccagtca
      121 cgtggtcgcc gactttgaac tgggtgaccg cctccccgac ggccgccacc tccgccaccc
      181 actcgtggcc atagatgaat ggcaggctgg gcggccaatg gggatagtag gccccgctga
      241 cgatgcgcag gtccgtgggg cagataccgc aggcccggac ccgcaagagc acttccttgg
      301 gaccgaaccg tggcaccggc acctcttcga tgtgaacctc gttccacgcc cgtatcaccg
      361 ctgcgcgcat ggttttcggc acagtaggca tagcaatctc ctggccatcc atgatgacta
      421 caatagggga tgcgggctga ggactgatag gggctttctc agtccccagc cctcgacacg
      481 cgtcccgttt aatacttctg caccgcatcg aacatctcca ccgcgtactt ggcgattaca
      541 tcggacactt tctctagaac ctggggaatc cgctcgacct tggctcgctc cagggagccg
      601 taccaaccgg tctctgccac ctcgcggaaa tcccggaaca tggtgtagac gtccttgcgc
      661 cggaaccgct cgtgccccac tctgccctcc tggagatccg aggggttggt ccctagttcc
      721 ggatggaaca gagccgggta gtagcacaga acctccatcg cctccatctc cgcggcgtgg
      781 gtccgctcca ggtccgggaa gagattgttg accaccaccc acacatccgc gatgacgatg
      841 cgcagatccc gatacttctg ctgcaggtca tccgccgcaa cgcgcagcgg agcgatgttt
      901 ccctcgtgcc cgttgctcac cagcactctg cgaatgccgt gctgatacag acagtcgacc
      961 acatcggtaa acaaggccat gaaggtcgag gacttcaggc tgatggtgcc cttccagggc
     1021 atatgatagg gagtgacacc cagcacggag aagggcacga acacggcctt caacttctca
     1081 gcgacccgct ttccgaacgc gatgcctgcc atggcatcgg tccccatggg caagtgcggg
     1141 ccgtgctgct ccgtgctccc cgtgggaatg actgcgatat tggtcttcga atagatcttg
     1201 tcggcttctg cggcgctgat ctcgaacagc gaattacccc aagccatgat gtcttcctcc
     1261 tgaatcctgt tatttattct tggcgattgc cttctggatc tggtccaggg ccgcctgacc
     1321 atccaacccc ttgctcttca tctggtccac ccacgcggcc cagaccggct tcgccgcagc
     1381 ctgccacttg gcctgctcgt cggccgggag catgatcact tccttgccca gcttcttgaa
     1441 attctccacg ccgatcgggt cggcctcgtc gaaggccccg cccagcatct gggcgccgaa
     1501 ccttccgccc atctcctcga acactttctg gacgtcgggc ggcaagctct tccagcggtc
     1561 cgggttcatg acgaggaact gggggacctg gaccaacggt gcgtaggtgg catagctcag
     1621 cacctcgtac agccggctgc tctggatccc ctgccagtcg aataccatcc cgtcgatgac
     1681 ccccttcgag atcccgtcat acatttcggg cgggctcatg gcgatggggt tgccgcccat
     1741 gttcttgatg aagttggtgg cgccccaagc cgtgacccga atcttctgtc ccttgatgtc
     1801 ctgcagggtc cgcaccggct tcttggccgt gttgacgaat tgcaccccgc tgctgaaggt
     1861 ggtcagtact ttcacgttcg accactcctt ctgcactgcc gggctacctt ggtagtagtc
     1921 ccatagcgcc gacgcgccgg ccttgccgct ctggacgccc agcatcggca ggttgtagac
     1981 ctcgctcagc gggaactggc cggcgtagaa gccgaccatc acccaaccga tgtcggcgat
     2041 tccgttctgg accatggcgt aggtgtccgg acccttgccc agggtctggg cggcaaagac
     2101 ctggatcttg accttgccgt tggtcttctc acccaccatg tccgcccact tctggaacgc
     2161 catgccggtc ctgccggtcg gcggctcatg gtgcgagaat ttcagattga tgacgctgct
     2221 cggcgcgggc gctgcggccc acgcaacggg agccagcagg aggccaacca gggccgccag
     2281 cacacccaga cgcagaaaca tccgtcctgt catcggtgtc cctccttctc tagccatgca
     2341 tctgccgcag atggttcagg ggcttccgct ccgagggata ctccgttccc cttctcacct
     2401 cctctcggaa ggccaactcg ctcaaccaga gcctacgagc gcagtcggta gccgcaccct
     2461 tcaactggtc cggcaggctg gtacgcctct ggctcagtcg gcaaccagct tctccccgct
     2521 gtgctccttc gggtcgatct ccatgccgga ggcacgagca atggccacca acatgtccgc
     2581 gacgatggcc aggcagaacg cgaccagccc gatcaccatc aggacctgga acggccatcg
     2641 cgggatgagc agcaccgtcg acacctgcct ctggacatag acctgcatgg cctctcgata
     2701 tccaacccac gtcatgggca ggaaaacccc cagggtcaga agcagggtca caacactcag
     2761 agccgcgcgc gtccgccggg agaggaactg gatcgccacg tcgacgttga tgtgggtgtc
     2821 ctcgagttgg gcgtaggcga gcgccaggaa caccgtgggg accatcaggt actcgacgac
     2881 ctcgaaggtc ccaagaatgg cactgtcaag aacgtcgcgc ccgatcacgt cgaaggctat
     2941 gaggaacatc attgccatca gggcaagggc gccgacggtt gccagggtga cgcacaggag
     3001 gcgaacccat cgccgaaacg tcagtatcca gcccatgccg ttcttccttt cttcacctac
     3061 ttcatcaggc ccggtaagaa cagcgccagc ttcgggaagg ccagcatgat gaacatgaag
     3121 atcaccaggt ccaggatgta gggccacatg taatagatga tctgcgacat cggaatgtcc
     3181 ctggccatgc cggagatgac gtacaggttg agccccaccg gcggggtgag ctgcccctgc
     3241 tccatggcca ccaccaccat caccccgtac cagatgggat cgaaacccag gccgaccacg
     3301 atgggaaaga aaaggggtgt ggtgagcacc accatggaga gcatgtccat gatgcaaccg
     3361 aggaaaaagt agaggatcag gatccccagg atgatcatga tgggcgggag cggcagggag
     3421 cgaaagtaag tcgccatggc ctccggcacc cccgtcacgg ccaggaagta gccaaagatg
     3481 aaggcgccga tcaagatcag gaataccatg gcggtgatct tggccgtctt ggcgaggcag
     3541 tctaccatgt tcctgaagtt gcactgccgc cgccagatca tgaagaggaa ggagccgaag
     3601 gcccccatgg cgcccgcctc ggtggcggtg aagatgccaa cccagatgcc ccccatgacg
     3661 aacaggaaca gcacgaccat gggccagctc cccctgagga caccgaactt ctcgcgccag
     3721 gtggtggctg gcccgcgggg ggcaatctca gggtttcgct tggcgataaa gtagatggtg
     3781 aggatgtaca gcgccgacag gatgatcccg gggatgatgc cagcgatgaa cagcttgccg
     3841 atggactgct ccgtgatcac cccgtaaatg atgaagccgg tgctgggagg gatcaggatg
     3901 gccagggtgc cggcggccgc gacgctggcg gcggcaaagc ccggcgcgta cttgtacttc
     3961 ctcatctccg gcagcgccac cgatccgaag gtggccgtgg aggccgcgct ggagccgcac
     4021 atggcgccga agccggcgcc ggcgccgatg gtggccatgg ccaggccccc aggcagatgg
     4081 ccgaaccatc ggtgggcccc gtcgtagagg tcccggctta tgccggactg gaaggacatc
     4141 tcacccatca ggatgaacag ggggatgacg gtcagggtgt agctggcgac tgtggagtag
     4201 gggacggtct tcaggagacc caaggctggg gtaatgccgg aaagcacagc gaagccgagg
     4261 aagcctgtca gcgccatggc gaccccgacg gagatgccgc tgaacagcag cgccaccatg
     4321 aacgcggtgc caagaatgcc gatctcgatg ggtgtcatgc gcgggaacct cccatgaaca
     4381 ggatcctgca cccgcatccg gtttggcgat gccgatgggg caccgcctgt catccatccc
     4441 cctccctggg ttgtggcccc ccggtcagcg ccctgccccg aagtctacgc aatgcttggc
     4501 tgatccatcc cccgctactc gatgaaaaac accaaggggt gggtccccag ctcctccagc
     4561 ctgggcttgg ctagcccact cgtaaccagt cttgccacct ctgaatcctt gtcgtccaga
     4621 tcgccgataa tcctcgcctc ggccgggcag gtggccacgc acgcgggttc cttgccctga
     4681 tcgagccggt gcacgcagaa ggtgcacttc tccaccaccc ccgcggtgtg ctgctgcgac
     4741 cccgcttcct cgacacgggt gaggcccttt cccgcaaagt attcctgcgg ctcgccatcg
     4801 ttgtagctac gcgcctcgta gggacatgcc atgatgcagt agcggcagcc aatgcacttg
     4861 gcggcttcga ccagcaccgg cccatcctgg cgtcgatagg tcgcgcctgc ggggcaggcc
     4921 tccaggcagg gcgcgtcctg gcagtgcatg catagcatgg gcaggaagcg gagccgcacc
     4981 ttgggatagg tgcccacctc gtactgcatc actttgctcc agtggatgcc ggggggcgtg
     5041 ccgtgttctt gcttgcaggc gacggtgcaa gcgttgcagc caacgcactt ccgcaggtcg
     5101 atgaccatgg cgtatctcat cgcgacgccc cggcccggta caccttcacc cgggggctcc
     5161 ggtcgatccc gccgtgcagc gggtcgaagc tgccctcggc gctagagagg agcgaattga
     5221 agtgaggacc gcgcctcgtg atcggattcc ggtgcatgga gcggaagcca tgaatggcgg
     5281 gaatccccac ggcgtccgga tgcatgagat ccgtgacacg gaggatcccc tccgttcggc
     5341 cgccccaata ggcctccacg accacgcgct ccccgtcggc gagccccttg cgctgcgccg
     5401 tactcttgtt gaggagcacg gtgaactggt acgggtcgca gcggtccacc acttcgttca
     5461 ggagaggatt gccggtctgg tcgccgcagt tcatgcggaa ctgcggtgtg gcccagttca
     5521 tcgcccagag gtcgtagtcg ggcggggcat tctcgtcggg gcgtcccacc caggatggga
     5581 tcggccgata gaaccgccac acgtcctcca tgtcctgccc cggaatggcg gttccatgcc
     5641 gctccaggtt ggctcgcagc gtatcgcccg tgcgcaggac gtgctcgaag tagaaggggt
     5701 ggcgggtggt gcccatgggg tagtagtaat agttatagga ctccttccgg ctgcgcatct
     5761 ggtactgcac ggcggagctg gtgaaggcct ggagcccgtt ttccacgccg tacttgccct
     5821 tgaggatccg ctcgatgatc tcgggtgtgc catagcgctt gtccagctcg agggcgtgag
     5881 gggccttcag ctcgtagaag cggttgagct gatcgttcag ccctccctcg ccatacagga
     5941 agcccacgcg ctccgccagc tcgatgtaga tatcatccgc ttgcatcgtg tcgtactgcc
     6001 gctccaccac tggcgtgcgc gccaggacgc cggtgacgcg cagcccgggc tcatccaccg
     6061 cctgcaccag gtgatccccc agtttttgga tcccgtagcg ttccagcagc gcgctctcgg
     6121 ggagcaggat atcggagaac tcggccgcct catcgtacag gtacgcgatg gtgacgacga
     6181 agggcacctt tgccagggcc gccacggcct cctcgggatt cgcgttgttg gtaacgggat
     6241 tgcctccata gatcatcagc gtatcgatcg ggtaggggac gccgtacttg acggggtcca
     6301 ggatcgcctt ccaggccatg tggggcatgg tgtggcggtt gggatagtac tggcggaggt
     6361 cgatgtcggg cgggaacttg aacggaatgg gggtggcctc gctcttcggc gccaccacgc
     6421 cgtcggggcc ggggctcagg accggcccgg tggccgagcc cagatacccc ccggggacgt
     6481 ccagggaccc caccagcatg cacagcatct tgctgaccag gtgctggtag gcgcctcctt
     6541 tcttgcccag ggtgccccgc tccgccttca cgatcgccgg ccggaaggga aacgcatagc
     6601 cgtccagctc gatcgtttcg ccgatgtgcg ccgcctccac gaactcccag gagaggcggc
     6661 ggacagtggc ggccgggacg gtggtgatcg gttcggccca ctcgggcgtg tacggcttca
     6721 cgtggtcttt cacgagctgg aaggcgacgg tgccagccgt atcgcccacc gggtatcgcc
     6781 ccgccagcgc gtaatccttg atggtcggat cgtcaaagac cttcggccga ccgtccaccg
     6841 gatcccagat catcggcttg ccggtggctc catccttcag gtagtagccg tcgtccccga
     6901 tgagatagac ggcattcgtc cttcgcttca ggaatggctc gtcgaacacc ttgatctcgt
     6961 gcaggaggac gtagagcatg gccagggaga aggcgagatc ggtgcctggc cggatgggga
     7021 cccactcatc ggcgagcgag gcttcgtggc tgcagcgggg atcgacgacg acgatccgca
     7081 tgccgcgctc ccgcgccctg gccagggcgc ggatcgagtg ggagctgccg atgttcggcc
     7141 ccagcgtacc cccgatcgtg atcaggtact ggcaatactc cacgtcttgt ttgtccagga
     7201 gcgtaccctg caccagggcg cagccatagt gcaccggaca gagcacaccg tgggtccgga
     7261 gcttgttcgg cgtaccgaag gccggcagga aggcgtcatc cgtcacaacc caccagtagc
     7321 ccccgaaccc ccatacatgg gccagcttcc gtggatcgtc ccgccggatc gccttcaggc
     7381 ggtccgccac cgtgctgagc gcctcttccc agctgatctc gacccagcgg gggtcctgcg
     7441 tgagcccctt cttcggattg gtgcgcttca tgggcgcctt cacccggtag gggttgtaca
     7501 ggttgaagag cgccgcctgc cccctggagc agagcttccc ttggctgacg ggattggtat
     7561 gatcgccctc gatcttgacc accaccccgt ccctcacctt gacccgcgcg gtgcaccagg
     7621 ccttgttgcc gcacatgcgg cagccggtca ccttccattc ttccctaggg cttt
//
LOCUS       DBNGZC010000962         2561 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2036954, whole genome shotgun sequence.
ACCESSION   DBNGZC010000962 DBNGZC010000000
VERSION     DBNGZC010000962.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2036954"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1869
                     /locus_tag="AC16G0_16620"
     CDS             <1..1869
                     /locus_tag="AC16G0_16620"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR5101281.1"
                     /translation="NMPGGYAGKILRVDLTSGRTWSHPWSPEEMRLYVGGAGLGAKIL
                     WEEVPATAGWDQPDNRLILATGPLAGLPLWGTGGLSVVTRGALTNGATSTQANGFFGA
                     CLKYSGYDAIVIQGEASRWVYLYINDDVAELRDAAHLLGKDTWETQDALQAEHGLAGH
                     QMSVYSIGPAGERLVRFAAIQGDYGHVASKNGCGAVMGKKRLKAVAIVRGTQALRAAD
                     PRGLFQAADEITHDLRTNPTTRSLYEYGTLPGVLNLHSFGGLPIKNYTTNVPPPGTDM
                     SVWEAPSLRQGFDHRGHQCNACGMHHCHMQVIPDGLHKGELVDEPEYEGWSGAGWAIG
                     CTDPAAISWLNTQVDRACVDVNEFGWLVGWVMECQEKGYLTPEQLGGLKLPWGDAEAA
                     ARLLAMINRREGLGDILAEGVKRAAETLGGPAAECAIYTLKGSSPRGHDHRARWDELL
                     DTCTGSTGTLETGPPVHPTELGLPARINPFSPDAVARMVGRFMGRRHFEDSLGACIFT
                     TRTLLENMCRALNAATGWEVTKEEAMQIGRRAGALFRAFNVRCGIGPELEQPSHRYAS
                     VPTDGPAKGQSILPHWDHMLDVWYDCVGYDRKTGRPRAETLRALGLERLTPDVWGT"
     gene            1878..2369
                     /locus_tag="AC16G0_16625"
     CDS             1878..2369
                     /locus_tag="AC16G0_16625"
                     /inference="COORDINATES: protein motif:HMM:NF040152.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pycsar system effector family protein"
                     /protein_id="HIR5101282.1"
                     /translation="MSVGDPVSTHLSFLQSACGTAEGAIRLADEKVGYTLLALGILVA
                     SLSLRADRLLSLLIAPDQPLVLRALLVAGAAAFLGAAGVSFVYALRSRDLSVDPPTDV
                     PSTLSRLVSLKLEGFLEELTQNLYRRAEIAGRKLILLRQCLIWAAAALAAWTWVLLIS
                     LVA"
BASE COUNT          418 a          842 c          896 g          405 t
ORIGIN      
        1 aacatgccgg gcggatacgc ggggaagatc ctgcgagttg acctgacgag cgggcggacc
       61 tggtcccacc cctggtcccc cgaggagatg cgtctctacg tcggcggcgc agggttgggc
      121 gcaaaaatcc tgtgggagga agttccggcc acggcgggtt gggaccagcc ggacaatcgc
      181 ctcatcctgg ccaccggccc gctggccggg ctgccgctat ggggaacggg cggcctgtca
      241 gtcgtgaccc gcggcgcgct gaccaacggg gcgacctcca cacaggccaa cggcttcttc
      301 ggggcgtgtc tcaagtactc gggctacgac gccatcgtga tccagggtga ggccagccgc
      361 tgggtctacc tgtacatcaa cgacgacgtg gcggagcttc gcgacgcggc ccacctgctg
      421 gggaaggaca cctgggagac ccaggacgcc ctccaggccg agcacgggct tgccggtcac
      481 cagatgagcg tgtacagcat cggccccgcc ggggagaggc tggtgcggtt tgcggccatc
      541 cagggcgatt acgggcacgt cgccagcaag aatggctgcg gcgcggtcat ggggaaaaag
      601 cggctcaagg cggtcgccat cgtccggggg acgcaggcgc tccgggccgc ggacccgcga
      661 ggcctgttcc aggcggccga cgagatcacc cacgacctga gaacgaaccc caccacccgc
      721 tcgctgtacg agtacggcac cctgcctggc gtgttgaacc tgcacagctt cggaggtctc
      781 cccatcaaga attacacgac caatgtgccc cctccaggaa ccgacatgtc ggtctgggag
      841 gcccccagcc tgcgccaggg gtttgaccac cgggggcacc agtgcaacgc ctgcgggatg
      901 caccactgtc acatgcaggt gatcccggac ggcctccaca agggcgaact ggtagacgaa
      961 ccggagtacg agggctggtc gggcgccggc tgggccatcg gttgcaccga tccggcggcg
     1021 atctcgtggc tcaacaccca ggtggaccgg gcctgcgtgg acgtgaacga gttcggctgg
     1081 ctggtcggct gggtgatgga atgccaggag aaggggtacc tcactccgga acagcttggc
     1141 gggctgaagc tcccatgggg ggacgccgag gccgcggccc ggctcctggc catgatcaac
     1201 cggcgggagg gccttggcga catcctcgcc gagggggtga agcgcgcggc ggaaacgctg
     1261 ggagggcctg cggcggagtg cgccatctac acgctcaagg ggtcttcccc gcggggccat
     1321 gatcaccggg cgcgctggga tgaactgctg gacacgtgca ccgggagcac cggcaccttg
     1381 gagaccggtc cccccgtcca cccgacggag ctcgggcttc ccgccaggat caatccattc
     1441 agtccggacg cggtggcgag gatggtcgga cggttcatgg ggcggcggca tttcgaggac
     1501 agtctcggcg catgcatctt cacgacgcgc accttgctgg agaacatgtg ccgcgccctc
     1561 aacgccgcca cgggctggga ggtcacgaag gaggaggcga tgcagatcgg ccgacgcgcg
     1621 ggggccctct tccgcgcctt caacgtgcgc tgcgggatcg gcccggagct ggagcagccc
     1681 tcccaccggt atgcctcggt acctacggac gggccggcga aaggccagag catcctaccg
     1741 cactgggacc acatgctgga cgtctggtac gactgcgtgg gatacgaccg gaagaccggg
     1801 cgcccccgcg ccgagaccct ccgggcgctc gggctggagc gtctcacccc cgatgtgtgg
     1861 gggacctagg ccgatccatg tccgtagggg atcctgtttc gacccatctc tccttcctcc
     1921 agtccgcctg tgggacggcc gagggggcca tccgactggc cgacgagaag gtaggctaca
     1981 cgctcctggc actagggatc ctcgtcgcca gcctgagctt gcgggcggac aggctcttga
     2041 gcttgttgat cgctcctgac cagccgcttg tcctccgcgc cctcctggtc gccggtgccg
     2101 ccgcgttcct gggggctgcc ggcgtctcgt tcgtctatgc gctgcgaagc cgggatctgt
     2161 ccgtggaccc gccgacggac gtcccttcga cgctttcacg cctggtctcg ttgaagctgg
     2221 agggcttctt ggaggagctg acccagaatc tctaccggcg ggcggagatc gccggaagga
     2281 agctgatcct gctccgccag tgtctgatct gggcggccgc ggctctggcc gcgtggactt
     2341 gggtactctt gatcagcctg gtagcctgag cctcccgctt ggtatccgag gccgatggcc
     2401 ggttccggcc tccgaggatt gccctgacag cagatagcga tcacccacct gtccaggagg
     2461 tgcgcatgca cagccacgat cgtgccttca tgccggcgac agagctggca tccgcgattc
     2521 gcgccaggaa gatctcgccc ggggaagtgg tggaggccat c
//
LOCUS       DBNGZC010000963         2917 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2335318, whole genome shotgun sequence.
ACCESSION   DBNGZC010000963 DBNGZC010000000
VERSION     DBNGZC010000963.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2335318"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /gene="msrP"
                     /locus_tag="AC16G0_16630"
     CDS             complement(<1..925)
                     /gene="msrP"
                     /locus_tag="AC16G0_16630"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HIR5101283.1"
                     /translation="MLITRAPKFKASEITDHGIYVNRREFMLRAAALALWPSVAAAAP
                     PPSGQPLKAIRNAAFSVSDPPTKFENVTTYNNFYEFGVDKDDPSRLAHSLRTRPWTVK
                     VDGEVARAKTYDIDELLKLFPLEERVYALRCVEGWSMVIPWIGFPLASLLKQVEPTGQ
                     AKFVEFTTLLDPEQFPGQRKGLLDFGGLQWPYIEGLRLDEAMHPLTLLTVGMYGQVLP
                     NQNGAPIRVVVPWKYGFKSAKSIVRIRLVAKEPQTAWNKAAPQEYGFYSNVNPTVDHP
                     RWSQATERRIGEFMRRKTLMFNGYADQVASLY"
     gene            complement(952..1758)
                     /gene="pgeF"
                     /locus_tag="AC16G0_16635"
     CDS             complement(952..1758)
                     /gene="pgeF"
                     /locus_tag="AC16G0_16635"
                     /inference="COORDINATES: protein motif:HMM:NF014619.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HIR5101284.1"
                     /translation="MQSRILRASGDVVHAFSTRRGGVSQPPYATLNLGQNVGDDPQAV
                     QVNRRRFFGAFGIEPPQVVRVKQVHGDGVLIVDPALAKRVGFPQVLLDERYEFDALIT
                     NVPDLALVVGTADCLPLLIHDPVQGAAAAVHAGWRSTAKRIAAKAIQAMQEAYGTNPA
                     DCRVAIGPGIRGCCFEVDRQVTEPMSAALREWEAHAKPSRPGHWRLDLAGVNRAILQE
                     TGVRPERIEDLGMCTACRTDLFFSHRAEKGRTGRMMNLILLVGRRRGAGT"
     gene            complement(1797..>2917)
                     /locus_tag="AC16G0_16640"
     CDS             complement(1797..>2917)
                     /locus_tag="AC16G0_16640"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5101285.1"
                     /translation="VKYHEDGTVSEVVPDLGAPLPVHKRRLPQTDAYDTVSTVRTANT
                     EFGEMALLEVGKGCGRGCRFCMEGEIYRPVRHRHTDAILRAAEAALRQGRRIGLVGAC
                     VSDYPWIDELVAALRARGVDFSLSSVRADSLTPALVRGLLDSGTRTLTIAPEAGTERL
                     RGVIHKSLSDQRLFEAVDLIAESDVPSVKLYFMIGQPTETAEDIEGIIHVAKTARHRI
                     LHKRRDPTRQAEFSVGVSSFVPKPWTAFQWCAMAEIRALDEKIQHLRRELSRVGIHFT
                     QDVPKWAYLQGVLARGDRHVGELLHLALEHQGDWKRAFREWPRNPDFYACRERPLRER
                     FPWDHFEVGTKRERLETEYQRAMGLLPERPVKWKASLA"
BASE COUNT          481 a          990 c          948 g          498 t
ORIGIN      
        1 tgtacaggga ggccacctgg tcggcgtagc cgttgaacat cagcgtcttc cgccgcatga
       61 actccccgat tcgccgctcg gttgcctggc tccagcgagg gtgatccacc gtgggattga
      121 cgttggaata aaagccgtac tcctggggcg cggccttgtt ccaggcggtc tgcggctcct
      181 tggccaccag gcggatgcgc acgatggact tcgcgctctt gaacccgtac ttccacggca
      241 ccacgacccg gatgggggcg ccgttctggt tcggtaacac ttgcccgtac atccccacgg
      301 tgagcaaggt cagcgggtgc atggcttcgt ccagccgcag cccctcgatg tagggccact
      361 gcaggccgcc gaaatccagg agccccttcc gctggcccgg aaactgctcg ggatcgagca
      421 aggtcgtgaa ctccacgaac ttcgcctggc cagtcggctc gacctgcttt aggagcgacg
      481 ccagggggaa accgatccag gggatcacca tggaccaccc ctccacgcag cggagggcgt
      541 agactcgctc ttccagcggg aacaacttca gcaactcgtc gatgtcgtat gtcttcgccc
      601 tggcgacctc gccgtccacc ttgacggtcc agggtcgagt ccgaaggcta tgggcgaggc
      661 gcgagggatc gtccttgtcc acgccgaact cgtagaaatt gttgtaggtg gtgacatttt
      721 cgaacttcgt cggaggatcg ctgacgctga aggccgcgtt acgaatggcc ttgaggggct
      781 ggccggaggg cggcggtgcg gcggcggcca cggacggcca cagcgccagg gccgctgctc
      841 tcagcatgaa ctcccgccgg ttcacgtaga tcccgtgatc ggtaatctcg gatgccttga
      901 actttggagc acgcgtgatc agcatggata tctcccggcg aggccctgaa atcaggtccc
      961 cgcacctctt cgcctgccaa caagtaagat gaggttcatc atccgaccgg tgcgcccttt
     1021 ctcggcccgg tgggaaaaaa acaggtcagt ccggcaggcc gtgcacatgc ccagatcctc
     1081 gatccgttcg gggcgaacac ccgtctcctg caagatcgca cggttcacgc cggccaggtc
     1141 gagccgccag tggccggggc gcgagggctt ggcatgagcc tcccactccc ggagtgccgc
     1201 agacatgggt tcggtcacct gccggtccac ctcgaagcag cagccacgga tcccgggccc
     1261 gatggccacc cggcagtccg caggattcgt cccgtaggcc tcttgcatgg cctggatcgc
     1321 cttggccgcg atgcgtttag ccgtgctgcg ccagccggca tggaccgccg ctgcggcccc
     1381 ctggaccgga tcgtggatca agaggggcag gcagtcggcc gtgccgacca ccagcgcgag
     1441 gtcgggcacg ttcgtgatca gggcatcgaa ctcgtagcgc tcgtccagca agacctgggg
     1501 gaatcccacg cgcttggcca gggcaggatc gacgatcagg acgccgtccc cgtggacctg
     1561 cttgacccgc accacctgcg gcggctcgat gccgaaggct ccgaagaagc gccgccgatt
     1621 cacctggacc gcctgcgggt catccccgac gttttgaccc agattcagcg tggcgtaggg
     1681 gggttgactc accccgcccc gtcgggtgct gaacgcatgg accacgtcgc ccgacgctcg
     1741 caggatcctg gattgcacca gtgcagagaa ttcgaccggt cgatccacgg ggcccgtcat
     1801 gccagcgagg ccttccactt caccgggcgc tcgggcagga ggcccatggc gcgctggtat
     1861 tcggtctcca ggcgctcccg cttcgtcccg acctcgaagt gatcccacgg gaaccgctcg
     1921 cggagcgggc gctcccggca ggcgtagaaa tccggattgc ggggccactc ccggaaggcc
     1981 cgcttccagt ccccctggtg ttccagggcc aggtggagca gctccccgac gtgccggtcg
     2041 ccccgtgcca ggaccccctg aaggtaggcc cacttgggca catcctgggt gaagtgaatg
     2101 cccacccggg aaagctcccg ccggagatgc tggatctttt cgtccagcgc gcggatctcg
     2161 gccatggcgc accactggaa ggcggtccag ggcttgggga cgaaggagct gacgcccacg
     2221 ctgaattccg cctgacgcgt gggatcgcgg cgcttgtgca ggattcggtg ccgggcagtc
     2281 ttggccacgt ggatgatccc ttctatgtct tcggccgtct ccgtcggctg accgatcatg
     2341 aagtacagct tcacgctcgg gacatctgac tcggcgatga gatcgactgc ctcgaacagg
     2401 cgctggtcgc tcaggctctt gtggatcaca cccctgaggc gctccgtccc ggcctcggga
     2461 gcgatggtga gtgtccgggt gccgctgtcc aggagccccc ggaccagggc aggggtcagg
     2521 ctgtcggccc ggaccgagga cagggagaag tccacccccc gggcccgcag ggcggccacg
     2581 agttcgtcga tccagggata gtccgagacg caggccccca ccaggccgat ccgccggccc
     2641 tgccggaggg ccgcctccgc cgcgcgcagg atggcgtccg tgtgccgatg ccggaccggc
     2701 cggtagatct ccccttccat gcagaagcga cagccccggc cacacccctt ccccacctcc
     2761 aggagggcca tctctccaaa ctcggtgttg gccgtccgga ccgtggagac ggtatcgtag
     2821 gcgtcggtct ggggaagacg gcgtttgtgc accggcagcg gcgcccccaa atcgggcacc
     2881 acttcgctca cggtcccatc ctcgtggtac ttcacgt
//
LOCUS       DBNGZC010000964         3948 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_379660, whole genome shotgun sequence.
ACCESSION   DBNGZC010000964 DBNGZC010000000
VERSION     DBNGZC010000964.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_379660"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            473..904
                     /locus_tag="AC16G0_16645"
     CDS             473..904
                     /locus_tag="AC16G0_16645"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5101286.1"
                     /translation="MRRRSGHVAITLGTTIGEAARVMKERRIGCVLVEDGGKLVGIFT
                     ERDILTKLVGTGYDPARVQVDGVMTRNPETLTPEDPIAYAMQLMSVGGFRHVPLVDAD
                     QHPLDILSVKDIVDYLVEHFPREVLNIPPEPGKHARIPEGG"
     gene            complement(1138..1344)
                     /locus_tag="AC16G0_16650"
     CDS             complement(1138..1344)
                     /locus_tag="AC16G0_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HIR5101287.1"
                     /translation="MSRIVKHERNSPYKVEDSDAKFPIWICGCGLSKNKPFCDGSHKK
                     TRDEETDGTYVYDDQGRVKVKTEY"
     gene            complement(1637..1825)
                     /locus_tag="AC16G0_16655"
     CDS             complement(1637..1825)
                     /locus_tag="AC16G0_16655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR5101288.1"
                     /translation="MNNWEERVSRKKRLNARILQSRMRVIPGFQELGKQVFADGAISR
                     KHKELMALAVAVSQNCFD"
     gene            complement(2129..3181)
                     /locus_tag="AC16G0_16660"
     CDS             complement(2129..3181)
                     /locus_tag="AC16G0_16660"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HIR5101289.1"
                     /translation="MAAPLPEPQPVTRWQFVCLALGLLLFIGLMYGIGLGEVLRTFRR
                     LGWLTPLIVIPYFSAYILDSFGWWWVLTRDFTRGAEWPALPLPRLFAIRAAGEAVNAI
                     TPTAYLGGEPVKAWLLRQYDLPLVPGLASVLVSKTALMLTQGAYVLLGLLIALHEWQP
                     AIPLPLAAAAGLLLVTLACILLVGAQRRGPFRFLLAFSRRWTGRKAMLVSWEPDILAL
                     DQRLREFYDGSLHDFLICCGFHFLGWVVGSFEVYVILRMLGSPVDFVNAFSIEALSGV
                     AKLSAVIVPGSLGIQEGGQVLIFLAFGLGTPVAMTFGLLRRGRELLWIGFGLVVLIQH
                     QALGWLRGRRGASREA"
     gene            3296..3502
                     /locus_tag="AC16G0_16665"
     CDS             3296..3502
                     /locus_tag="AC16G0_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101290.1"
                     /translation="MGSLSTHFLKSVSRHLSQVPTDPDDLVIAAAQLTSLLDGLDRMA
                     ELDLLTVEPATVLLPSSEGPHVNG"
     gene            3489..>3948
                     /locus_tag="AC16G0_16670"
     CDS             3489..>3948
                     /locus_tag="AC16G0_16670"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR5101291.1"
                     /translation="MSMDELTRLSIADAAERIRTGRLSPVDLTRAYLDRIQRLNGGCR
                     AYLTVLSAEALAAATAAEQEIVRGHYRGPLHGIPVALKDLVMTRGIRTTCGSLILRDW
                     VPETDATVAARLAQAGAVLLGKLNMHEFAYGPTGVNPHYGTPRNPWGHDRM"
BASE COUNT          728 a         1283 c         1238 g          699 t
ORIGIN      
        1 ctccttcgcg gacctcccga gaactccgga agggctcagc aagccaccgc gggtttctcg
       61 ggatgggtcg cgacccattt cccgagcatg tgtggaacct gcgaaagccg tggtttccgg
      121 ggtagccgaa gcacccaggg cctattgtac tccaggtgtg cccctacggg aagcaacctc
      181 cccgcaactt ttcgttggcg cttctccggc aagcgaccta tcatcagcca cgtggtggtt
      241 ttccgcctgt gccaggatca ggcgggcgca ggaaccgagg ggggccgggt ctccagcccg
      301 acgaggggcc agacaggcga gctggacctt ggggctcgtc cagaggacgg cacagggagg
      361 ggatagcatg aacatggaag aggcgatcta tgatgaggat ttgcgttttg aggatgacgc
      421 gagcccccac aaggtgctgg actaggacct cttcggagag cccatcagga ccttgcggcg
      481 gcgctcgggt cacgtggcga tcacgctggg gaccacgatc ggcgaggcgg cccgggtgat
      541 gaaggaacgt cgaatcgggt gcgtcctggt cgaggatggg ggcaaattgg tggggatctt
      601 caccgagcgg gacattctca ccaagctcgt cggcaccggg tacgatccgg ctcgggtcca
      661 ggtggacggg gtcatgaccc ggaatccgga gacgctcacc ccggaggatc ccatcgccta
      721 tgcgatgcaa ctcatgagcg tgggcggatt tcgccatgtc cccctggtgg atgcggacca
      781 gcaccccttg gacatcttgt cggtgaagga tatcgtggac tatctggtgg agcacttccc
      841 gagggaagtc ctcaacatcc cgcccgagcc gggaaagcat gctcgcatcc ccgaaggggg
      901 ctgagccttc ggtttcaggt cacccttggc tcggaggaca cccgggtctt gccttgccgg
      961 tacggccatg gctcgccgca gccttgtctt tcttcccgag cggcccgcgc gacactcgcc
     1021 attgttcccc gtgaggggct gaatatccgg ggaagcgagt aggcccaagg cacggacgcc
     1081 gacgggcctc atcctcgggg tgcccgccgg cgttcagcag ggcggaggtt cttgcggcta
     1141 gtattcggtc ttcaccttca cacgcccctg atcgtcatac acgtacgtcc cgtccgtctc
     1201 ctcatcccgg gtcttcttgt gggagccgtc acaaaagggc ttgttcttcg acaggccgca
     1261 gccgcagatc cagatgggaa acttggcgtc gctgtcttcg accttgtagg gactgttccg
     1321 ctcgtgcttc acgattctgg acatgggttc ctcctctctc gactctttcc ctgatgctcg
     1381 gcgggcaagt tgtgtgtacg atcaccctcg tgattgcata ccaccacacg agcgccccgt
     1441 caactcacct tcgcgattcc gccgggccat ctcacggctt gcccttggct tctcggatct
     1501 cctccaagac ctcgaaggca tatcgcacgg acttcaccgc cagcgtgccg cccatgagcg
     1561 accccacatc cagcgcctcc gccaattcgg catcggtccc gcccaggtcg aggacctcct
     1621 cgacacggtg ttcgattcag tcaaaacaat tctgggagac cgccacggcg agcgccatca
     1681 actccttgtg ctttcggctg atggccccgt ctgcaaagac ctgtttgccc agttcctgaa
     1741 atccagggat gacccgcatc cgactctgga ggatccttgc gttcagtcgt tttttccgcg
     1801 acacccgctc ctcccagttg ttcatgggtt gcctcctatg atcgtgggtt gaggattttt
     1861 cttcccggac gcgatttttt tcttgcgtcc ggaaagtctt gcatgtaggt tttgggtaca
     1921 ccagaaagga ggtgatccag tgggaatatt aaactggaca ggtgaggtga ttgtaacata
     1981 ggcgtttccc cgcttaggcg aacgcccggg gtttcgtcta tggaatccca gcaatcgccg
     2041 ggcctcgaag cctccggcct tgtccaccgg ggcggccagc ttgccgtgaa atcttcgagg
     2101 cctttttctt tgtccggacc cgccccgttc acgcctcgcg actcgctccc cgtcgccccc
     2161 tcaaccatcc cagtgcctga tgctggatca gaacgaccaa gccgaatccg atccacagaa
     2221 gttcccgtcc ccgccgcagc aggccgaaag tcatggcaac cggcgtcccc agaccgaagg
     2281 ccaagaagat caggacctgg ccgccttcct ggatccccag gctccccggc acgatgacgg
     2341 cagagagttt ggccacgccc gacagcgcct cgatggaaaa ggcgttgaca aaatccaccg
     2401 ggctccccag catccgcagg atcacgtaca cctcgaagga acccacgacc caccccagga
     2461 agtgaaaccc gcagcagatc aggaagtcgt gaaggctgcc gtcgtagaac tcccgtaagc
     2521 gctgatccaa ggccaaaata tccggctccc acgacaccag catggccttt cgaccggtcc
     2581 agcggcgcga gaaagccagg aggaagcgga atgggcctcg acgctgggcc ccaaccagga
     2641 ggatgcacgc cagggtgacc agcaggaggc cggccgccgc cgccaggggc agcgggatgg
     2701 ctggctgcca ctcgtgcagc gcgatcaaga ggcccaacag aacataggcg ccctgggtga
     2761 gcatcagggc ggtcttggat accagcaccg acgccaggcc ggggacgagg gggagatcgt
     2821 actgacggag gagccacgcc ttcacgggct cgccgccaag gtaggcggtg ggggtgatgg
     2881 cgttgaccgc ctcgccggcc gcacggatgg cgaagagccg cggaagggga agcgcgggcc
     2941 actcggcccc gcgcgtgaag tcgcgggtca ggacccacca ccagccgaag ctgtccagga
     3001 tgtacgcaga aaagtagggg atcacaatca gcggggtgag ccagcccaat cgccgaaacg
     3061 tccgaagaac ctcgccgagc ccgatcccat acatcaggcc aatgaagagg agcagcccca
     3121 gggccaggca cacgaactgc cagcgggtga ccggctgcgg ctcgggcaga ggcgccgcca
     3181 ctagcggcct cctgccatgg cgacgcaccc gcgtccccgg ggctttcggc tggacagccc
     3241 ttcggggatc ttggtagagt aacgcactcg aatggatcta tcccaggagg agaccatggg
     3301 ctccctctcg actcacttcc tgaagtcggt cagtcgccat ctctcacagg ttccgacgga
     3361 cccggacgat ctggtgatcg ccgccgctca gctcacctcg ctgctcgacg ggttggaccg
     3421 catggccgaa ctggatctcc tcaccgtgga gccggcgacc gtgctcttgc cgtcatcgga
     3481 gggcccccat gtcaatggat gagctgaccc gcctgagcat cgcggatgcg gccgagcgga
     3541 tccgcacagg tcgcctctct cccgtggatc tcacacgggc ctacctggat cggatccagc
     3601 ggctgaatgg ggggtgccgg gcatacctca cggtcctgag cgcggaggcg ctggccgccg
     3661 cgaccgcggc ggagcaggag atcgtccggg gacactaccg gggcccgctg cacgggattc
     3721 ccgtcgccct gaaggacctg gtcatgacgc gggggatccg gacgacctgc ggctccctga
     3781 tcctgcggga ctgggtgccc gagacggatg ccacggtggc cgcgcgtctc gcccaggcgg
     3841 gagccgtcct cttgggcaag ctcaacatgc acgagttcgc ctacggcccg accggcgtga
     3901 atccgcatta cgggacgccc aggaatccgt ggggtcatga tcgcatgc
//
LOCUS       DBNGZC010000965         2977 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_473514, whole genome shotgun sequence.
ACCESSION   DBNGZC010000965 DBNGZC010000000
VERSION     DBNGZC010000965.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_473514"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="AC16G0_16675"
     CDS             <1..431
                     /locus_tag="AC16G0_16675"
                     /inference="COORDINATES: protein motif:HMM:NF024639.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HIR5101292.1"
                     /translation="HRVDVMPGIADLVRALASRQDALVGLLTGNIERGARSKLGPTGL
                     LPFFRVGAYGSDDADRRRLPAVARERAEALVGHPFPFEQMTIIGDTPLDIDCARSCGA
                     VAVAVATGQHAADELAACVPDLLFSDFSDVAASVCALMQL"
     gene            460..1398
                     /locus_tag="AC16G0_16680"
     CDS             460..1398
                     /locus_tag="AC16G0_16680"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fpg/Nei family DNA glycosylase"
                     /protein_id="HIR5101293.1"
                     /translation="MPELPDILVYVDALEQRILGRPLERIRLASPFLLRSVEPPLETA
                     HGKTVRNLRRLGKRIVIGLDGDLFLVLHLMIAGRLHWKSRGTKIQGKVGLAAFDFPSG
                     TLTLTEAGTKKRASLHLVGGERLLRDHDPGGLDVLHADAATFRAALTRENHTLKRALT
                     DPRLFDGIGNAYSDEILHRARLSPVQLTQRLDADEIERLFGATHGTLSGWIERLRREA
                     GNGFPEHVTAFREGMAVHGRYGKPCPDCGSPVQRIVYAENETNYCARCQTGGKLLADR
                     ALSQLLRRDWPRTLEELEDMRAAARTIPAEARRPVP"
     gene            1837..>2977
                     /gene="hpnR"
                     /locus_tag="AC16G0_16685"
     CDS             1837..>2977
                     /gene="hpnR"
                     /locus_tag="AC16G0_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008169 - C-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid C-3 methylase HpnR"
                     /protein_id="HIR5101294.1"
                     /translation="MKVLCCHPSGLMYTKIFLRLEPLGVELVAAAARAARHDVRLLDL
                     QVLTHADYFAVLDDWKPDAITFGLNYLANIPEVIDLAKETKRRLPDSFFFVGGHSASF
                     TAQEILEHASEAIDCVVKGEGEEIVPLVLLAAANDRRSIRTIPGIVTLDGDGPPPRKI
                     ESLDSLMPARDLLPKRHKYFIGVLDPAASIEFTRGCPWDCVFCSAWTFYGRSYRKASP
                     EKVGEELQSIREPGVFIVDDVAFIQAEHGFAIGREIEKRNIRKRYYLETRGDVLLRNK
                     EVFQYWKRLGLATMFLGLEAIDEEGLKASRKRVKLSTNFEALEFARSLGISVAVNIIA
                     DPDWDERRFAIIREWALSIPEIVSVSVNTPYPGTETFVSESRTLTT"
BASE COUNT          520 a          980 c          955 g          522 t
ORIGIN      
        1 gccaccgggt ggatgtgatg ccgggcatcg ccgacctcgt gcgggccctg gcctcccgcc
       61 aggatgcctt ggtgggtctc ctcacgggga atatcgagcg gggcgcccga tcgaagctcg
      121 ggcccacggg tctcctgccg ttcttccggg tcggggccta tggctcggac gacgcagacc
      181 ggcgccggct cccggccgtg gcccgagagc gcgccgaggc cctggtgggg cacccgtttc
      241 ccttcgaaca gatgacgatc atcggcgaca cgccgcttga catcgactgc gcccggtcct
      301 gcggggcggt ggccgtcgcg gtcgccacgg gtcagcacgc agccgacgag ctggcggcct
      361 gtgttcccga tctcctcttc agcgacttct ccgacgtcgc cgcttccgtg tgcgcactca
      421 tgcagttgtg agacagacct ccgcggatga catgccgcca tgccggagct tcccgacatc
      481 ctggtgtatg tcgacgccct ggagcagcgg atcctcggcc ggccgctgga acgcatccgt
      541 ctcgcgagtc cgttcctgct ccgctccgta gagcccccgc tcgagaccgc acacggcaag
      601 acggtccgca accttcggcg cctcggaaaa cgtatcgtca tcggtctcga cggcgatctc
      661 ttcctcgtcc tccacctgat gatcgccggg agactccact ggaagtcccg cggaacaaag
      721 atccagggga aggtcggtct ggccgccttc gatttccctt cggggacatt gaccctgacg
      781 gaggccggca cgaagaagcg cgcatccctg cacctggtgg ggggagagag gcttctccga
      841 gatcacgacc ccggagggct ggacgttctc cacgcggatg ctgcgacctt ccgcgctgcg
      901 ctcacgcggg agaaccacac gctgaagcgg gccctgacag accctcgcct cttcgatgga
      961 atcggcaacg cttactctga cgagatcctt caccgcgcac ggctctctcc ggttcaactg
     1021 acccagaggc ttgacgcaga cgagatcgag cgactcttcg gcgccaccca tggcaccctc
     1081 tccgggtgga tcgagcgcct ccgccgggag gcgggaaacg ggttccccga gcatgtcacg
     1141 gcgtttcgcg aagggatggc cgtccatggg cgctatggga agccctgccc cgattgcggc
     1201 tcccctgtcc aacgcatcgt gtatgcggag aacgagacga actactgcgc ccggtgccag
     1261 acgggaggca agctcctggc ggatcgcgcc ctgtcacagc ttctgaggcg ggactggcca
     1321 aggacgctgg aggagctgga agacatgcgg gccgccgcac gcacgatacc cgcggaagct
     1381 cgccggcccg ttccgtgacc tctcccgcag atgcgcaatg gcacttccgg ccgacgttcc
     1441 tcccgcccca acggcaacag ggcctcggca gggccgcaaa atagtgcttg gcgtcctctc
     1501 gttatttgag tattagtgtt cagtccgact cttgcagttc ttgtatgggc gcatgggggg
     1561 aatggctccc catgcaccca gccatagaga tccttgctcg aagcccatga cggcggcgta
     1621 gggctgtgat cacacttccg accaggaagg gtgcatgaca gtcttagcag gatgccgaaa
     1681 aactcgtagt tgcgcaggct gctcaaaaag gtccggatgc aaggcggcgc gcacagggcc
     1741 gagtgaggcg tacttaggcg tacgtcgcag cgagcgcccg agcgcgccaa cgtagcagat
     1801 gggccttttt cagcagcctg ttgggggagg ggaaccatga aggtgttgtg ctgccatccc
     1861 agcgggctga tgtacaccaa gatcttcctg cggctcgagc cgcttggggt cgagctggtg
     1921 gctgcggcag cccgggcggc caggcacgat gtccgattgc tcgatctgca ggtcttaacc
     1981 cacgccgact actttgcggt cctggacgac tggaagccgg acgctataac cttcggcctc
     2041 aactacctgg ccaacatccc cgaggtcatc gatctcgcca aggagacgaa gcggaggctc
     2101 cccgactcct tcttcttcgt tggcggccac agcgcttcct tcaccgcgca ggaaatcctg
     2161 gagcacgcca gcgaagccat cgactgcgtg gtgaagggcg agggcgagga gatcgtcccg
     2221 ctcgtcctct tggcggcggc caacgaccga cgaagcatcc ggacgattcc gggcatcgtg
     2281 accctggacg gggacggccc gcccccacgg aagatcgaga gtctggacag cctgatgccc
     2341 gctcgcgatc tgctccccaa gcgccacaag tatttcatcg gcgtcctgga tcccgccgcc
     2401 tccatcgagt tcacgcgagg ctgcccgtgg gactgtgtct tctgcagcgc ctggaccttc
     2461 tacggccgga gctaccggaa ggccagcccc gagaaggtgg gcgaggagct ccagagcatc
     2521 cgggaacccg gcgtcttcat cgtggacgac gtggccttca tccaggcgga gcatggattc
     2581 gccatcggcc gcgagatcga gaagcggaac atccggaaga ggtattacct ggagacgcgt
     2641 ggcgatgtcc tgctccggaa caaggaggtc ttccagtact ggaagcggct gggcctcgcg
     2701 accatgttcc tgggtttgga ggccattgat gaggagggtc tcaaggcgag ccgcaagcgg
     2761 gtgaagctgt ccacgaactt cgaggccctt gaattcgctc gctcgctcgg gatctcggtg
     2821 gcggtcaata tcatcgccga cccggactgg gacgagcggc ggttcgccat catccgggag
     2881 tgggcccttt ccatccccga gatcgtgagc gtgagcgtga atacgcccta ccccgggacc
     2941 gaaacgtttg tctcagagtc ccgcacactc accaccc
//
LOCUS       DBNGZC010000966         2388 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1057292, whole genome shotgun sequence.
ACCESSION   DBNGZC010000966 DBNGZC010000000
VERSION     DBNGZC010000966.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1057292"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..955)
                     /locus_tag="AC16G0_16690"
     CDS             complement(17..955)
                     /locus_tag="AC16G0_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101295.1"
                     /translation="MHREPVDSTFYEPNIIVAPKHEALRPDGPEAYGVERSDVSGETR
                     QGRNVVMTWAEAKQSAHPLAKDGYKFIFHTPKYRHGAHTMPIDTDMVAVLFGPFGDLY
                     RHDRRSPFVAEGYVDIHPSDAKALGVEDGDYVWIDSDPADRPFRGWQNNKKDYEFARL
                     LCRARYYPGTPRGVTRMWFNMYGTTPGSHEGQAKRPDGLAKNPRTNYQAMFRSGSHQS
                     ATRGWLKPTWMTDSLVRKGLFGQGVGKGFLPDVHCPTGAPRESIVKITKAEPGGLEGK
                     GLWRPAALGLRPRYESEEMKRYLAGEFLGGDPRKGR"
     gene            complement(1052..>2388)
                     /locus_tag="AC16G0_16695"
     CDS             complement(1052..>2388)
                     /locus_tag="AC16G0_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin oxidoreductase"
                     /protein_id="HIR5101296.1"
                     /translation="VILREKLYDADYVRRWTDLPLLVRMDTLKYLRAAEVFGTAPASL
                     SNQTRVLKDGESEPPPGQHREMLIPEKLRGEWGDFVFWDQKAQAPQAITRDGVGAKSP
                     VTDPLLQGAVEVSLKDGKKVRCRPVFDLVQEYAAHFDPKTVEELTWAPAAAVESLARH
                     IAKVPGTTLFAIGMGPNQFFNNDNKDRDIFLLAALTGNVGKVTGNVGSYAGNYRVALF
                     NGVPQYINENPFDLELDPGKPARPRQYWRSESAHYYNHEDHVLRVGKKLLTGSTHIPT
                     PTKSLWFANANSILGNVKWHYNTVVNVLPRIEMIAVNEWWWSTSCEWADVVFAVDAWC
                     ELKHPDMTASVTNPFLSIFPRTPLKRTFGTIGDIEVLATVGEKLADLTQDKRFAECWK
                     FVREGKTDVYLQRILDHSSNTKGYRIQDLEAKALQGIPTIMLNRTNPKAVGY"
BASE COUNT          354 a          748 c          832 g          454 t
ORIGIN      
        1 ggacgcgcgc catggcctac ctccccttcc tgggatcgcc gccgaggaac tccccggcaa
       61 ggtacctttt catctcctcg ctctcgtagc gggggcgcag gcccagcgcg gcgggccgcc
      121 acagcccctt gccctccagc ccgccgggct cggccttggt gatcttcacg atggattcgc
      181 ggggggcgcc ggtggggcag tgcacgtcgg gcaggaagcc cttccccacg ccctggccga
      241 agaggccctt gcgcaccagg gagtcggtca tccaggtggg cttgagccag ccgcgggtgg
      301 cggactggtg cgagccagag cgaaacatgg cctggtagtt cgtccgcggg ttcttggcca
      361 ggccgtccgg tcgcttggcc tgcccctcgt gcgagccggg ggtcgtcccg tacatgttga
      421 accacatgcg ggtcaccccg cgcggcgttc cggggtagta gcgggcccgg cagagcagcc
      481 gggcgaattc gtaatccttc ttgttgttct gccagccccg gaaggggcgg tcggccgggt
      541 ccgagtcgat ccagacgtag tcgccgtcct cgactcccag cgccttggcg tcggatggat
      601 ggatgtccac gtagccctcg gccacgaaag gcgatcgcct gtcatgccgg tagaggtcgc
      661 cgaaggggcc gaagaggacc gccaccatgt cggtgtcgat gggcatggtg tgggcgccgt
      721 ggcgatactt gggggtatgg aagatgaact tgtagccgtc cttggccagg gggtgagccg
      781 actgcttggc ctcggcccag gtcatgacga cgttccgtcc ctggcgcgtc tcgcccgaga
      841 cgtcggagcg ctcgaccccg taggcctcgg ggccgtcggg acgaagcgcc tcgtgcttgg
      901 gggcgacgat gatgttcggc tcgtagaagg tggagtccac gggttcccgg tgcactggca
      961 ggttctcgcc ggcctggatg aactcgtcct cctcgcggta cgactccagc cgtccgctct
     1021 tggtgtacca ggggcgggag tcgttgacct gctagtagcc caccgccttc ggattcgtcc
     1081 ggttcagcat gatggtcggg attccctgca gggcctttgc ctccagatcc tggatccggt
     1141 agcccttggt gttgctggag tggtccagga tccgctggag gtacacgtcg gtcttgccct
     1201 cgcggacgaa cttccagcac tcggcaaagc gcttgtcctg cgtgaggtcg gccagcttct
     1261 cgccgaccgt ggccaggacc tcgatgtccc cgattgtccc gaaggtgcgc ttgagcggcg
     1321 tccgcgggaa gatcgagagg aacgggttgg tgacggaggc ggtcatgtcg ggatgcttca
     1381 gctcgcacca ggcgtccacg gcgaagacca catcggccca ctcgcaggac gtggaccacc
     1441 accactcgtt gaccgcgatc atctcgatcc tggggaggac gttgaccacg gtgttgtaat
     1501 gccacttcac gttgccaagg atcgagttgg cgttggcaaa ccagagggac ttcgtggggg
     1561 tcgggatgtg ggtcgatccg gtgaggagct tcttccccac gcggaggaca tggtcctcgt
     1621 ggttgtagta gtgggccgac tcggaccgcc agtattgcct gggccgggcg ggcttccccg
     1681 ggtccagttc cagatcgaac gggttctcgt tgatgtactg gggaaccccg ttgaagaggg
     1741 cgacgcgata gtttccggcg tacgagccga cgttgccggt caccttaccc acgttgcccg
     1801 tgagggcggc caggaggaaa atgtcccgat ccttgttgtc gttgttgaag aactggtttg
     1861 ggcccatgcc gatggcgaag agggtggtgc cggggacctt ggcgatgtgg cgggccagcg
     1921 attccacggc agcggccggg gcccaggtca attcctccac ggtcttcggg tcgaagtggg
     1981 ccgcatactc ctggacgaga tcgaagaccg ggcggcagcg gaccttcttg ccgtccttga
     2041 gggatacctc caccgcgccc tggagcaagg gatcggtcac cggcgatttc gcgccgacgc
     2101 cgtcgcgggt gatcgcctgc ggcgcctgcg ctttttgatc ccagaagacg aagtcacccc
     2161 actcgccccg cagcttctcg gggatcagca tctcccggtg ctgacccggc ggcggctcgc
     2221 tctcgccatc cttgagtacc cgcgtctgat tggacagact ggccggcgcg gtgccgaaga
     2281 cctcggcggc gcgcaggtac ttcagcgtgt ccatccgcac caggaggggc aggtcggtcc
     2341 agcggcggac gtagtcggcg tcgtacagct tctcccgcag gatcacat
//
LOCUS       DBNGZC010000967         4442 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1744014, whole genome shotgun sequence.
ACCESSION   DBNGZC010000967 DBNGZC010000000
VERSION     DBNGZC010000967.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4442)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4442)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4442
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1744014"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="AC16G0_16700"
     CDS             <1..784
                     /locus_tag="AC16G0_16700"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5101297.1"
                     /translation="RRVLVVGGGPAGMMAALTAAARGHRVTLCESKARLGGQLLMGDV
                     PPHKEEIRTLREHLVGQIATAGVETRLQTTATEELIKEIGAEVVIVATGAEPVQPRLP
                     VEGGRLVSAWDVLAETESAGAKVVVLGGGEVGCELAEYLAAQGKEVMILELLPELAGN
                     MEHRGRALLVQRLRELGVQVLLQCRMREVRGATVTYEQGGLMHRIEGVDTVVSAVGSA
                     AHRPLGETLQVPGATVHSIGDCVKPRRILEAIREGFELAYSI"
     gene            872..1984
                     /locus_tag="AC16G0_16705"
     CDS             872..1984
                     /locus_tag="AC16G0_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015375616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR5101298.1"
                     /translation="MRSKAVVLHAFNQPLVIEEIEVDLPKKDEVMVRVVNAGVCRSEV
                     PAIQGKRAIPLPIVLGHEGAGVVEKIGEDVKAVKPGDHVILVWRVPCGRCNSCAIGRP
                     ALCEEAAKASGKGSMPDGTLRFSKDGKPVYHFLTASCFQEYTVVHEKAVIPIPKDFPL
                     HCAAIMGCGLLTGVGAVINTAQVRAGSTVAVFGAGGVGLSAILGARLVNARKIIAVDI
                     KDSKLDTALSLGATDAVNSAKVDPVKAIRDLTGGRGVDYAFEVIGLGKVFEQAFEATG
                     RSGTTTMVGSPPPEETFTLSARLLYSQERTVKASYYGSARFYADIPWLVGLYQAGRLP
                     IDKLISRHFPLDQYNEALQALEAGEVARSVLDVTPL"
     gene            2078..2848
                     /locus_tag="AC16G0_16710"
     CDS             2078..2848
                     /locus_tag="AC16G0_16710"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HIR5101299.1"
                     /translation="MGARGLYMMDCGGGEFDYGVLVAMQRPGEKVLSPFSACLIDTDD
                     GPVLVETGVNPDGLKDPLAAAGERARSLKLVLKEEDDIRARLKEIGLKPADVRRVILS
                     HMHWDHAGGCKFFSHATFVVQRAEYRFALYPDKAFSRPYFRPLFEGMSNLDLREGDGE
                     VVPGVWVLSTPGHTPGHQSVLVSLPESGKMLLAFDAIDTWANVELETPSGIPWNAALA
                     LDSLRRLVQLAKRENATLVPGHEPGCWSKMKRSPDCYR"
     gene            2929..3981
                     /locus_tag="AC16G0_16715"
     CDS             2929..3981
                     /locus_tag="AC16G0_16715"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR5101300.1"
                     /translation="MAKTFADRRALVSLALVCAVVFFAAIPAKAAGPIILRAGASDPE
                     GTTAPALSIGKLCELANKLSNGELNAQAFYQSLGVEQQLAAAVKGGSVDIGFTAVSNL
                     ARFTDAFLQFDLPFLFRNDKDYIEVLKNHPAGKKAIAQFEKDLGVKMLLITSHTYDGE
                     VSGTNLETRNKPVKVPADIKGLKLRTASTPVEIALIKAYGANPTPVDYGQLYSALQQG
                     VVDGNAATPLPPSVSIKLYEVAKYYVATGFRYNLLPVYINQKKFDSLTPFQQKALLDA
                     AAQTEALAGQYARDKVKSSIETLEKNGVHIYHPNKEEMAQWLSVREKVWQQVVDEFKG
                     KVDLNVANEIFKMYPR"
     gene            4104..>4442
                     /locus_tag="AC16G0_16720"
     CDS             4104..>4442
                     /locus_tag="AC16G0_16720"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101301.1"
                     /translation="MGKTYVERRFLIGIALICSLVVFGAIPAAQAGAPIILRGGATDA
                     EGTTAPAQAFAKYCELANKLSNGELNVQPFYQSLGVDQQLAAAVKGGSVDIGYGSSSN
                     RARFTDAFLQF"
BASE COUNT          838 a         1337 c         1521 g          746 t
ORIGIN      
        1 ccggcgcgtc ttggtcgtgg gcggggggcc ggccggcatg atggcagcgc tcaccgccgc
       61 ggcgaggggg cacagggtca ctctgtgcga gagcaaggca cgcctgggcg ggcaactgtt
      121 gatgggagac gtccccccgc acaaggagga gatccgcact ctgcgggaac acctggtcgg
      181 gcagatcgca acggccgggg tcgaaacgcg tttgcagacc accgcgaccg aagagctgat
      241 caaggagatc ggcgcggagg tggtgatcgt ggccaccggc gctgagccgg tgcagccacg
      301 cctccccgtc gaggggggac ggctcgtctc ggcctgggac gtgctcgcag agacggagtc
      361 ggccggggcg aaggtcgtgg tgctgggcgg aggggaagtg ggttgtgagc tggcagagta
      421 cctggccgcc cagggcaagg aagtgatgat cctggagctg ctgccggaac tcgccggcaa
      481 catggaacac cgggggcggg cgctgctggt gcagcggctc cgggagctgg gggtgcaggt
      541 gctgctgcag tgccgcatgc gcgaggttcg gggggcgacg gtcacctacg agcagggcgg
      601 gctgatgcac cggatcgagg gggtcgatac cgtggtctcg gccgtgggct ccgccgccca
      661 ccggccgctg ggagagacgc tgcaagtccc aggggccacg gtccactcca tcggagactg
      721 cgtgaagccc cggcgcatcc tggaagccat acgcgagggg ttcgaactgg cctactctat
      781 ctgaaacacg acggtacggg cgcggacacg gcgacgggcc ttggctcagg ccgttcggat
      841 ggcgagtcga aaagagaaac agggggtgtc catgcgttcc aaagcggttg tccttcacgc
      901 gttcaaccag ccgctggtga tcgaggagat cgaggtcgac ctgcccaaga aagatgaggt
      961 tatggtccgg gtggtgaatg cgggagtgtg ccggagcgag gtgcccgcca tccagggtaa
     1021 gagggcaatc cccttgccca tcgtgctggg gcacgagggg gccggcgtcg ttgaaaaaat
     1081 tggcgaggat gtcaaggcag taaaacccgg cgaccacgtc atcctggtgt ggcgcgttcc
     1141 ctgcggccgc tgtaattcct gtgccatcgg tcggcccgct ctctgtgagg aggccgccaa
     1201 ggccagtggc aaggggagca tgccggacgg gacactgcgc ttctccaagg atgggaaacc
     1261 cgtctatcac ttcctcaccg cctcctgctt ccaggagtac acggtcgtcc atgaaaaggc
     1321 cgtgatcccg atccccaagg actttcccct gcattgcgcc gccatcatgg gctgtgggtt
     1381 gttgaccggg gtgggggcgg tgatcaacac ggcccaggta cgagcgggaa gcacggtggc
     1441 cgtcttcggc gcgggcggcg tcggactcag cgccatcctg ggcgccaggt tagtgaacgc
     1501 caggaagatc atcgcggtgg acatcaagga cagcaagctg gatacagctc tctccttagg
     1561 ggcgaccgac gccgtcaact ccgccaaggt cgatcctgtg aaagccattc gggacctcac
     1621 gggcggacgg ggggttgact acgcttttga agtgatcgga ctggggaagg tgttcgagca
     1681 ggccttcgag gccaccggcc gctccgggac gacgaccatg gtgggctccc cgccgccgga
     1741 agagaccttc acgctctcgg cccggctcct ctactctcag gagcggaccg taaaggcttc
     1801 ctactacggt tcggcccggt tctacgccga catcccctgg ctggttggtc tgtaccaggc
     1861 tgggaggctc ccgatcgata agttgattag ccggcacttt ccactggacc agtacaacga
     1921 agccctgcag gccctggagg ccggagaggt cgcccgcagt gtgctcgacg tgacaccact
     1981 gtgacctccg ggggtgccgg gcaaccggcg gggcatccgc ttgttgttct ggcccggcga
     2041 gcacccgggt aactccgctt tcgagagagg gtgagccatg ggggcacgag gcctgtacat
     2101 gatggattgt ggcgggggcg agtttgacta cggcgtcctg gtggccatgc agcgcccagg
     2161 cgagaaggtg ctttcgccct tcagcgcctg cctgatcgat acggacgacg gccccgtgct
     2221 ggtggagacc ggggtcaacc ccgatgggct caaagatccc cttgcggcgg cgggggagcg
     2281 ggcgcgctcc ctgaagctgg tgttgaagga ggaagacgac atccgcgcgc gcttgaagga
     2341 gatcgggctg aagccggcgg acgtgcgccg cgtgatcctg tcccacatgc actgggatca
     2401 tgcgggagga tgcaagttct tttcccatgc aaccttcgtg gtgcagcgcg ccgaataccg
     2461 cttcgccctc tacccggaca aggccttcag ccggccctac ttccggccgc tcttcgaggg
     2521 gatgtcgaat ctggacctgc gggagggcga cggagaggtg gtgccgggtg tctgggtgct
     2581 ttccactccg gggcacacgc caggccatca gtcggtcctc gtctccctcc cggaatcggg
     2641 aaagatgcta ctggcctttg acgccatcga cacctgggcc aacgtcgagc tggagacgcc
     2701 gagcgggatc ccctggaatg ctgccctggc cctggatagc cttcggcgtc tcgtgcagtt
     2761 ggccaagcgg gagaatgcca cgctggtgcc cggacacgag ccgggctgct ggagcaagat
     2821 gaaacgctcg ccggactgct accgttgagc agccagacgg gagaaaggag gcgatgatcg
     2881 agagcctgag ctgaacgttg aaacctggtc gcatgaagga ggggaacgat ggcaaagacc
     2941 ttcgctgata gacgagcact ggtatccctc gcgctagttt gcgccgtggt tttcttcgcg
     3001 gccattccgg ccaaggcggc cgggcccatc atccttcgcg ccggcgcctc cgaccccgaa
     3061 gggacgaccg cgccggccct gtccatcggg aagctgtgcg agctggcaaa caagctgagc
     3121 aacggggagt tgaatgccca ggcgttctac caatccctgg gggtggaaca gcaactggcg
     3181 gcggccgtga agggtggcag cgtggacata ggctttaccg ccgtctccaa cctggcccgg
     3241 ttcacggatg ccttcctcca gttcgacctc ccattcctct tcaggaacga caaggattat
     3301 atcgaggtat tgaagaatca tccggcgggc aagaaagcga tcgcccagtt cgaaaaagac
     3361 ctgggggtca agatgctgct gatcacgagt cacacctatg acggtgaggt cagcggcacc
     3421 aacctggaga cgcgaaacaa gccggtgaag gtcccggccg acatcaaggg gctaaagctg
     3481 cgcaccgcca gcacccccgt cgagatcgcc ttgataaagg cctatggcgc caaccccacg
     3541 ccggtggatt acggccagct ctactctgcg ctccagcagg gcgtggtcga cggcaatgcc
     3601 gcgacccccc tgcccccctc cgtgtccatc aagctgtacg aggtcgccaa gtactacgtg
     3661 gccacaggct tccgctacaa cctgcttccc gtctacatca accagaagaa gttcgattcc
     3721 ctcacgccct tccagcagaa ggccctcctg gacgcggcgg cccaaaccga ggccctggcc
     3781 gggcagtatg cccgggacaa ggtgaagtcc tccatcgaaa cgttggagaa gaacggtgtc
     3841 cacatctacc atccgaacaa ggaagagatg gcgcagtggt tgtccgttcg ggagaaggtc
     3901 tggcagcagg tggtcgacga gttcaagggc aaggtcgacc tcaacgtcgc caacgagatc
     3961 ttcaagatgt acccccgcta gacagatttg ctgaagcccc cgccggactg cgtccgttga
     4021 gcagcccggc gagagaggac gggaagtggc cagggtcctg agccgagagg gaaattctct
     4081 gatcgcatga aggagacata acgatgggaa agacctacgt tgagaggcgg ttcctgatag
     4141 gcatcgcctt gatatgctca ctcgtagtct tcggagccat cccggccgcg caggccggag
     4201 cgccgatcat cctgcgtggc ggcgccaccg acgccgaagg cacgacggcc ccggcccagg
     4261 ccttcgccaa gtattgcgag ctggcgaaca agctgagcaa cggggagctg aatgtccagc
     4321 cgttctacca gtccctcggg gtggaccagc aattggcggc agccgtgaag ggcggcagtg
     4381 tggatatcgg gtatgggtct tcatccaacc gggctcgctt cacggatgcc ttcctccagt
     4441 tc
//
LOCUS       DBNGZC010000968         1742 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1353434, whole genome shotgun sequence.
ACCESSION   DBNGZC010000968 DBNGZC010000000
VERSION     DBNGZC010000968.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1353434"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..>1742
                     /locus_tag="AC16G0_16725"
     CDS             303..>1742
                     /locus_tag="AC16G0_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HIR5101302.1"
                     /translation="MTSAHLGGQASGTGEPYRALLAVSEAIASHRDLPALFHELAGRL
                     HHVVRFDYLALVLHEAATNTMRLHALGASEPTPYIPAIVLPVENDPVGLVWQIQQPLI
                     ISNVAEESRWPQFLERVQPHGVQSLCMLPLTTARQRLGTLVFASRRPAAYEAADAGFL
                     QLVANQVAVAVENALAFQEIEAAFREIQALKDKLAKENAYLEEEVRTEHNFGEIVGES
                     AALRRVLKEVETVAPTGSTVLIRGETGTGKELIARALHELSPRRDRTFVKLNCAAIPT
                     GLLESELFGHEKGAFTGAISQKAGRFELAHQGTLFLDEVGDIPPELQPKLLRVLQEQE
                     FERLGGTKTIKVDVRLVTATHRDLARMVAECRFREDLYYRLNVFPLVLPPLRERPDDI
                     PRLVRHFTQRFARRMGRRIEAIPSAVMEALVRYPWPGNIRELQNVIERAVILSPGPSL
                     QVPPGDLQPAPAQARTSPAAPSTLADAERD"
BASE COUNT          284 a          583 c          600 g          275 t
ORIGIN      
        1 ggtgacgtcg ctggggtagt ggtgcgttct catcccagtt ctacgatgct caaccccctg
       61 atgttcccct tatgggacag actgtaagct cggatggatt gcccgcgccg catggagaat
      121 taccgcggct gcgcggcgca ccagtcgtcg gaggggcgca acgaagcaga ccaggggggt
      181 ggcggtcggc cggaggtgga ccaccggccg accgagcggt tggcttgaat gaatcggggc
      241 atagctatcc gacggccccc caggaatccg ttcgaggtgc cgctcacgcc ggggtttccg
      301 ccatgacaag cgctcactta ggcggacagg catctggcac tggcgagccc taccgggcct
      361 tgctggccgt ctcggaggcg atcgcctcgc accgcgacct gcccgcgctg ttccacgagc
      421 tggccggccg gctccatcac gtggtgcgct tcgattacct cgccctggtc ctgcacgagg
      481 cggcgaccaa caccatgcgc ctgcacgccc tgggggcctc cgaacccaca ccgtacattc
      541 ccgcgatagt tcttcctgtt gaaaatgacc cggtggggtt ggtgtggcag atccagcaac
      601 cactgatcat ctcgaacgtg gcggaggaga gtcgctggcc gcagtttctg gaacgggtgc
      661 agccgcatgg agtccaaagt ctttgcatgc tcccgttgac cacggcccgg caaaggctgg
      721 gcacgctggt cttcgcgtcg aggcgtccgg ccgcctacga ggcggcggac gcgggcttcc
      781 tccaacttgt cgccaatcag gtggccgtgg cggtcgagaa cgccctggcc ttccaggaga
      841 tcgaggcggc cttccgggag atccaggcgc tcaaggacaa gctcgccaag gagaatgcct
      901 acctggagga ggaggtccgc accgagcaca acttcggcga gatcgtcgga gagagcgccg
      961 ccctacgccg ggtgctgaag gaggttgaga cggtcgcccc cacgggatcc acggtgctca
     1021 ttcgtggcga aacgggcacg ggcaaggagc tcatcgcccg cgccctgcac gagctgagtc
     1081 cccggcggga ccggaccttc gtcaagctca actgcgcggc catccccacg gggctgctgg
     1141 agagcgagct gttcgggcac gagaagggcg ccttcaccgg agccatcagc caaaaggcgg
     1201 ggcggttcga gctggcccac caggggacgc tgttcctcga cgaggtgggc gacatccccc
     1261 cggagctgca gcccaagctg cttcgcgtgc tgcaggagca ggagttcgag cgcctcggcg
     1321 gcacgaagac gatcaaggtg gacgtgcggc tggtgacggc gacgcaccgg gacctggccc
     1381 ggatggtggc cgaatgccgt ttccgtgagg acctctacta ccggctgaac gtcttcccgc
     1441 tggtgctgcc gccgttgcgg gagcggccgg acgacatccc gcggctggtc cgccatttca
     1501 cacagcggtt cgcccggcgg atgggccggc ggattgaggc catcccgtcc gcggtcatgg
     1561 aggcgttggt gcgctacccg tggccgggca acatccggga gctgcagaac gtcatcgagc
     1621 gggcggtcat cctctccccg ggcccgtcgc tccaggtacc ccccggcgac ttgcagcccg
     1681 cccccgcgca ggcccggacg tcgcccgctg cgccgagcac cctggccgac gccgaacggg
     1741 ac
//
LOCUS       DBNGZC010000969         2641 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_473643, whole genome shotgun sequence.
ACCESSION   DBNGZC010000969 DBNGZC010000000
VERSION     DBNGZC010000969.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_473643"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="AC16G0_16730"
     CDS             complement(<1..980)
                     /locus_tag="AC16G0_16730"
                     /inference="COORDINATES: protein motif:HMM:NF014042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HIR5101303.1"
                     /translation="MTSAASEGVPRLVALGGRSGPSLLVQGFKDSGWAVTAIVTTTDC
                     GSSSGVIREQFGVPAPGDIRTVLAAAADLPSEFRLLADLFEFRFRPATESSLQNMALG
                     NLILTALVEELGDFDRAVEAAARLLNSWAEVLPVPSSPATLCARLADGTVVRGEVNVR
                     ASGKPPILEVFLCPADVQVAEGCLRAIREADLVVLGPGGLYCSVLSSLLAMPIRQAIR
                     ESRAKTAYVCNSTTQPGQTDGFDSVAHVREMLQYLGEGQLDYVLLNTYVPEDEVIAAY
                     EKDGVHFMWVTPEEIRRVKELGLTPVVGHFIESDWRGKRVLHKLDTIRHDP"
     gene            complement(977..2401)
                     /gene="glmU"
                     /locus_tag="AC16G0_16735"
     CDS             complement(977..2401)
                     /gene="glmU"
                     /locus_tag="AC16G0_16735"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIR5101304.1"
                     /translation="MIPAGNTSAVAPTRDVCAIVLAAGLGTRMRSSLVKVLHPLNGLP
                     MVAHVLDLCRRMGVKRTLGVIGHQSERLREALGDFPVEFVEQAEQRGTAHAVLQAEAA
                     LQGFEGDILVINGDVPLLGEALIQRLLATHRKSGADATLVTTRLPNPTGYGRVVRDRR
                     GVFRKIVEEVEATPAERQVREINAGIYCFRGPRLFTCLHGVKPSPVKGEFYLPEVLPM
                     LRKAKGKIATVQAEDPREVLGINTRAELAEAYGILRQRVVARLMEGGVTCLDPATTHV
                     SMLATVGEDTTLYPNVQLEGKSSIGTGCTIHAGCRIRDSHLGNRVMVLDGCVILESEI
                     ADDCSIGPFAHLRPRCQVKRRAKVGNFVEVKKSVIGEGSKVPHLTYVGDTTVGERVNI
                     GAGTITCNYDGFGKHQTIIEDEAFIGSNANLVAPVKIGRGAIIAAGSTITQEVPPDAL
                     AFGRAQQVNKEGRAAEIRNAKAKK"
BASE COUNT          451 a          902 c          808 g          480 t
ORIGIN      
        1 ggatcgtggc ggatcgtgtc cagcttgtgc aatacccgtt tcccccgcca gtcgctctcg
       61 atgaagtgtc ccacgaccgg ggtgaggccc agttccttca ctcgccggat ctcctcgggc
      121 gtgacccaca tgaagtgtac cccgtccttc tcgtaggccg cgatcacctc gtcctcgggc
      181 acatacgtgt tcaggagcac gtagtcgagt tgcccctcgc ccagatactg aagcatctcc
      241 cgcacgtggg ccacgctgtc gaaaccatct gtctgcccag gttgggtcgt ggagttgcag
      301 acgtaggctg tcttggctct ggactcccgg atcgcctgcc ggatcggcat tgccaggagg
      361 ctggagagga cactgcagta taatcctccg ggaccaagga cgaccaaatc tgcctcgcgg
      421 atcgcccgaa ggcagccctc tgccacttgt acgtccgccg ggcacaagaa cacctccaga
      481 atggggggct tccccgacgc ccggacgttc acctctcccc ggacaactgt accgtctgcc
      541 aggcgcgcgc acagcgttgc cgggctggaa ggaacgggga gcacctctgc ccaagagttc
      601 aggagtcgtg cggccgcttc gacggcccgg tcgaaatcgc ccagctcctc gaccaatgcg
      661 gtgaggatca ggtttcccag ggccatattt tgcagactgg attcggtggc cggtcgaaaa
      721 cggaactcga acaggtctgc caggagccgg aattccgacg gcagatcagc cgccgccgcc
      781 agcaccgtcc gaatgtcgcc cggcgcggga acgccgaact gctcccggat gaccccgctg
      841 ctgctcccgc agtccgtcgt ggtgacgatg gccgtgactg cccagcccga gtctttgaat
      901 ccctgtacca gcagcgaggg gcccgatcgc ccgcccaggg ccacgagccg ggggacaccc
      961 tccgacgcgg cactcgtcac ttcttggcct ttgcgtttcg gatctcggca gcccgcccct
     1021 ctttgttgac ttgctgcgcc cggccgaagg ccagggcgtc cggcggcacc tcttgggtga
     1081 tggtcgaccc ggccgcaatg atggccccgc gcccgatctt gaccggggcg acgaggttgg
     1141 cgttggagcc gatgaaggct tcatcctcga tgatggtctg gtgtttgccg aagccatcgt
     1201 aattgcacgt gatggtcccc gccccgatgt tcacgcgctc gcccaccgtg gtatcgccca
     1261 cataggtcaa gtgcggaacc ttggatccct ccccgatcac cgacttcttc acctcgacga
     1321 agttccccac cttggcccgg cgtttgacct ggcagcgcgg tcggaggtgg gcaaaaggac
     1381 cgatcgaaca gtcgtcggcg atctcgctct ccagaatcac acacccgtcc aggaccatca
     1441 cccggtttcc caggtgcgag tcccggatcc ggcaccccgc atggatcgtg cacccggttc
     1501 cgatgctgct cttgccctcg agctggacgt tgggatagag cgtggtgtcc tccccgaccg
     1561 tggccagcat cgagacgtgc gtggtcgccg gatccaggca ggtaaccccc ccctccatca
     1621 ggcgggccac cacccgttgg cgcaggatgc cgtaggcctc ggccagttcg gcccgcgtgt
     1681 tgatgcccag cacctcccgg gggtcttcgg cctggactgt ggcgatcttc cccttggcct
     1741 ttcgaagcat gggcaggacc tcgggaaggt agaactctcc cttcacggga gagggcttca
     1801 cgccgtgcag gcaggtgaac agacgggggc cgcggaagca atagatgccg gcgttgatct
     1861 ccctcacctg ccgctctgcc ggcgtggcct ccacctcttc aacgatcttg cggaaaacgc
     1921 cccggcgatc ccgcaccacg cgtccgtagc cggtgggatt gggaagccgg gtcgtgacca
     1981 gggtcgcatc ggccccggac ttccggtgcg ttgccagcag gcgttggatc aatgcctcgc
     2041 ccagaagcgg gacatcgccg ttgatgacca ggatatctcc ctcgaagccc tgcagggcgg
     2101 cctcggcctg cagcacggca tgggccgtgc cccgttgctc ggcctgctcc acgaactcca
     2161 cggggaagtc ccccaaggcc tcccgcagcc gctcgctctg atgcccgatg accccgagtg
     2221 ttcgcttgac gcccatgcgc cggcacaagt ccaggacgtg ggcgaccatg ggcaggccgt
     2281 tgagtggatg gagaaccttc accagacttg agcgcatccg ggtccccaga ccggccgcca
     2341 acacaatggc gcagacatcc cgcgtggggg ccacagcact tgtatttccg gctggaatca
     2401 tcggcgcccc tcgtcctgtg tccggattag aaacttgtca cccgatcccg gttcgaaagc
     2461 gaatgataac ataccaaatt ttgacgaaaa agaggagttt ctcgacgggg aatgacacgt
     2521 gggacctagt gtcccgagtt gaaagttcgg ttgctaatac ggcggcctgt gacagcaccc
     2581 ctgcggccgc cgggctccgt cgagaaccgg tcagccttgc tccggcgccg ccgccccaga
     2641 g
//
LOCUS       DBNGZC010000970         4320 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2724024, whole genome shotgun sequence.
ACCESSION   DBNGZC010000970 DBNGZC010000000
VERSION     DBNGZC010000970.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4320)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4320)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4320
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2724024"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="AC16G0_16740"
     CDS             <1..865
                     /locus_tag="AC16G0_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018205237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5101305.1"
                     /translation="FRKEHQWQIKELLFTLRLLGRNPSAVAGFVIIAAMVFMAVCAPL
                     LTSYNPIRISLAERLNPPSSVHPFGTDELGRDLLSRIMYGARISLRIGILVVLIAGGF
                     GAVIGAISGYVGKQADNLIMRVMDVILSFPPLVLALALAAALGPDLNNAILAVAFVMI
                     PKFARLVRGETLAVKERQFIGAARAAGATGLWIIQHHILPNCLSSVIVLATLVLGEAI
                     LIAASLSFIGLGAQPPTPEWGAMVSVGRKFLMDQWWYATFPGLFILTTVIGFNILGDA
                     LRDILDPRIRR"
     gene            870..1862
                     /locus_tag="AC16G0_16745"
     CDS             870..1862
                     /locus_tag="AC16G0_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5101306.1"
                     /translation="MADPLLEIKGLSVRFFTYQGTVRALEEVDLTIERGEVLGLVGET
                     GCGKSVMARSILRLIPDPPGKISHGEILFKGEDILKLRARRLREIRGNDISMIFQEPM
                     SSLNPVFRVGNQIEEVIMLHQKVDRRRARAAAVEMLQQVNMPDPEQVLRKYPHELSGG
                     MRQRVMIAMELSCRPDLLIADEPTTALDVTVQGQVLTILNDLVRRLGISVLFISHDLG
                     VIAQLCDRVSVMYAGKIVETAPVAGLFAEPAHPYTRGLLQAIPSLDEERDSLRVIPGI
                     VPRLVEPPGGCRFHPRCAERFAPCDRLVPRPVEVGPGHEVACHLYGEREAGKRS"
     gene            1859..2875
                     /locus_tag="AC16G0_16750"
     CDS             1859..2875
                     /locus_tag="AC16G0_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008481922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5101307.1"
                     /translation="MTPVIEVVDLVKHFPVHGGFLGRRVEHLRAVDSVSLTIQPRETL
                     GLVGESGCGKTTLGYLILNLLRPDRGKVLYRGTDIGALQGEGLRRVRRNLQIVFQDPQ
                     SSLDPRMLIKKTVGYPLEVNRIARGEEVVARVRSILAEVGLDEEHMYRYPHEFSGGQR
                     QRIGIARALITGPEFIVFDEPTSSLDVSVQAQILNLVKELQQRHGYAYLFVSHNLSVI
                     KHVSHRVAVMYLGKIVEAAPRQALFEHPLHPYTRALLASVPKPDPGKRHTLGVLTGDV
                     PSPINVPTGCRFHPRCPEVMNECRHVEPAGMEVEPGHWVACHLYGLARSQLSQKEKID
                     AARP"
     gene            2859..3965
                     /locus_tag="AC16G0_16755"
                     /pseudo
     CDS             2859..3965
                     /locus_tag="AC16G0_16755"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            3982..>4320
                     /locus_tag="AC16G0_16760"
     CDS             3982..>4320
                     /locus_tag="AC16G0_16760"
                     /inference="COORDINATES: protein motif:HMM:NF014766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HIR5101308.1"
                     /translation="MMVTASAHSNLAIQDMKIYRFDIPLREVFTIATMSLSRAQNLLV
                     ELRTNEGISGWGEASSFHSIAGETQAINIAAAKELKPYVVGKNPLAINSLIQQMEEYL
                     PHNTTLKSAID"
BASE COUNT          739 a         1382 c         1410 g          789 t
ORIGIN      
        1 gttccgcaaa gaacaccaat ggcagatcaa ggaactgctc ttcacccttc ggctgctcgg
       61 gcggaacccc tccgccgtag ccgggtttgt cattatcgcc gccatggtgt tcatggccgt
      121 ctgtgccccc ctgctcacct cctacaatcc catccggatc agtctggcgg agcgcctcaa
      181 ccctccttcg tccgtgcacc ccttcggtac ggatgagctc ggaagggacc tcttgagccg
      241 catcatgtac ggcgcccgca tctccctgcg catcggcatt ctggtcgtgc tgatcgcggg
      301 ggggttcggc gccgtcatcg gggccatcag cgggtacgtg gggaagcagg ccgacaacct
      361 catcatgcgg gtcatggatg tcattctctc ctttccgccc ctggtgctgg ccctggccct
      421 ggccgccgcg ctgggacccg acctgaacaa tgcgatcctg gccgtggcct ttgtcatgat
      481 cccgaagttc gcccgactgg tgcggggcga gaccctggcg gtcaaggagc ggcaattcat
      541 cggagccgcc cgggccgcgg gggccacggg gctgtggatc atccagcacc acatcctgcc
      601 gaattgcctt tcctcggtca tcgtcctggc gacgctggtt ctgggagagg cgatcctgat
      661 cgcggcctcg ctcagcttca tcgggctcgg ggcgcagccc ccgacgccgg aatggggggc
      721 catggtcagc gtgggtcgga aattcttgat ggatcaatgg tggtacgcca ccttccctgg
      781 tctgttcatc ctgacgaccg tcatcgggtt caacattctt ggagatgccc tgcgtgacat
      841 cctcgatccg aggatccggc gatagtgcca tggctgatcc cctgctggag atcaagggtc
      901 tctcggtccg gttcttcacc taccagggaa ccgtccgcgc tctcgaggag gtggatctga
      961 ccatcgaacg gggagaggtc ctggggctgg tcggtgagac ggggtgcggg aaatccgtca
     1021 tggcccgctc catcctgcgg ctcatccccg acccgcccgg gaagatcagc cacggcgaga
     1081 tcctcttcaa gggggaagac atcctgaagc ttcgggcgcg aaggctgcga gagatccgcg
     1141 ggaacgacat ctcgatgatc ttccaggagc ccatgagctc cctcaacccg gtgtttcgcg
     1201 tcgggaacca gatcgaggaa gtgatcatgc tgcaccagaa ggtggatcgc aggcgggcca
     1261 gagcggcggc ggtggagatg ctccagcagg tcaacatgcc cgatccggag caggtcctca
     1321 ggaaatatcc ccacgagctg tccgggggga tgcggcagcg tgtcatgatc gccatggaac
     1381 tctcgtgccg gcccgacctc ctgatcgccg acgagcccac gaccgccctg gatgtgacgg
     1441 tgcagggcca ggtgttgacc atcctgaacg atctggtgag aaggctcggg atctccgtcc
     1501 tcttcatctc ccatgacctg ggcgtcatcg cccagctttg cgaccgggtg agcgtcatgt
     1561 acgccggcaa gatcgtggag acggcgccgg tggccgggct gttcgccgaa ccggcccacc
     1621 cctacacccg ggggctgctc caggccattc cctcgctgga tgaagagcgg gattcgctgc
     1681 gggtcattcc gggaatcgtt ccacgactcg tggagccgcc aggcgggtgc cggtttcatc
     1741 cccgctgcgc ggagcgattc gcaccgtgcg accggctcgt ccccagaccg gtagaagtcg
     1801 gccccggcca tgaggtagcg tgccatctct acggggagcg agaggcaggg aagcgttcgt
     1861 gacgcccgtc atcgaagtcg tcgatcttgt gaagcacttc cccgtgcacg gcggctttct
     1921 gggccgccgg gtggagcacc tgcgggcggt cgactcggtg agcctcacga tccagccgcg
     1981 agagaccctg gggttggtcg gggaatccgg gtgcggcaag acgacgctgg ggtatcttat
     2041 cttgaacctg ctccggccgg accgagggaa ggtcctgtac cggggcacgg acatcggcgc
     2101 gcttcagggc gaagggctcc ggcgagtccg gcgaaatctc cagatcgtct ttcaggatcc
     2161 ccaatcctcc ctcgaccccc ggatgctcat caagaagact gtggggtacc ccctcgaggt
     2221 caatcgaatc gcacgcgggg aggaggttgt tgccagggtg aggagcatcc tggccgaagt
     2281 gggcctggat gaggagcaca tgtaccgcta tccccacgag ttcagcggag gccagcggca
     2341 gcgcatcggt atcgccaggg cgcttatcac cggacccgag ttcatcgtct tcgacgagcc
     2401 gacgtcgtcc ctggacgttt ccgtccaggc ccagatcctc aacctggtga aggagctgca
     2461 gcagcgacac ggctacgcct atctcttcgt ctcccacaat ctgagcgtca tcaagcatgt
     2521 gagtcaccgg gtggccgtca tgtacctggg caagatagtc gaggcggcgc ccaggcaggc
     2581 gttgttcgaa catcccctgc atccgtatac ccgtgccctg ctggcctccg tgcccaagcc
     2641 ggaccccggg aagcgccaca ccctgggggt tctgacgggg gatgtgccca gcccgatcaa
     2701 cgtgccgacg ggatgccgat tccatccgag gtgcccggag gtgatgaacg agtgccggca
     2761 cgttgagccc gccggcatgg aggtggagcc ggggcactgg gttgcctgcc acctctacgg
     2821 gctggcaagg agccagctaa gtcagaagga gaaaatcgat gccgctcggc cctgagcttc
     2881 gtttcctgga tcggacgaaa gtcctgcacg aggggaaacc ttacctgttc ttcggcggga
     2941 cagactacca tcgcctggcg agtcacccgg gtgttctccg ggcgctccgc gaggcggccg
     3001 agagcgacgg cctgagctgt gctggatccc ggaccaccac ggggaaccat cccctgctcc
     3061 ccgagctgga acggaaggtg gcggcgttcc tgggggccgg agaggcggcc ctctgctcca
     3121 gcgggtacct ctcgaacgcc gtcgctttgg aggcgatggc cggcgagttt cagcgcttct
     3181 tcatcgacgg cagcgcccac tcgagcctcg ccgcgcccgc aggccggctc tcccgagagg
     3241 cggtcgtcac cttccggcat gcggatccgg aggacctcac gcgggagctg aagacacgcc
     3301 tgcgtcccgg cgagaggcct ctggtcctca cggacggggt cgtgccggtg gatggggaga
     3361 tgcctccgct ccgaatctac tgggaggcgg tgcgagattt cggcggcgcg ctcctcgtgg
     3421 atgacgccca cggcatcgcc gtcgtggggc cgacggggaa gtgaagccca gaggaggcag
     3481 gactccctgg gggaagcttc atccagaccg ggaccctgtc caaggggctg ggtgcttttg
     3541 ggggcctcgt ggcgggccag gacggcctgg cgggcaagat cgtggagagg agtttggcct
     3601 tcgtcggagg aaccccgatt ccaccgcctc ttgccgcggc cgccctccgc tcgatcgaga
     3661 ttctccatga gagtccgggg ctgatcacgg gcctccgcgg ccgcatgctc cgcgttcggg
     3721 agcgtatccg agccatgggt ttccggattc cctcctcgcc ggcgccgatc ctctcgatca
     3781 cccaccacga cgtgaacaag aatcaacgcc tccgctcgct ccttttcgag cgagggatct
     3841 atcccccctt catccgctac cctgggagtc cgcccggcgg gaccttccgg ttcgccgtct
     3901 cgagtgcgca tgcagaggag gaggtggacc ttcttcttga gacgatcggg atgtcttgcg
     3961 attgagagag ggagaaccga gatgatggtc acggcttcag cccattccaa cctggcgatc
     4021 caggacatga aaatttatcg gttcgacatt ccgctcaggg aggtcttcac cattgccacc
     4081 atgagcctca gccgcgcgca aaatcttctc gtggagctac ggacgaacga aggcatcagc
     4141 ggctggggag aggcatcttc tttccactcg atcgccggag agacccaggc gatcaatatc
     4201 gccgcggcca aggagttgaa gccgtacgtg gttggcaaga atcctctggc gatcaattcc
     4261 ctgatccagc agatggagga gtacctgcct cacaacacga cgctgaagag cgccatcgac
//
LOCUS       DBNGZC010000971         2858 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2137994, whole genome shotgun sequence.
ACCESSION   DBNGZC010000971 DBNGZC010000000
VERSION     DBNGZC010000971.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2137994"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /gene="plsX"
                     /locus_tag="AC16G0_16765"
     CDS             complement(<1..842)
                     /gene="plsX"
                     /locus_tag="AC16G0_16765"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIR5101309.1"
                     /translation="MTRTETVRVALDAMGGDSGPEVNVEGAVAAAREFGAAVVLVGAE
                     EEIRRQMRRHDVQGLSLSVCHAPEAVEMGESPLAALRHKKHSSIRIGLELIKRSQAEA
                     FVSAGNTGAVMATALVVLGTLPGVERPAIAVVVPTLTGQAVLLDVGANVDCKARHLVQ
                     FAIMGNVYARDVLGISRPRVGLLSIGEEESKGNELTREAFKELEDEASLNFIGNVEGR
                     DVFNGSSDVIICDGFTGNVALKISEGLADTMLHLLREELRTDLRSRMGSLLLIPAFRR
                     FKRRV"
     gene            complement(863..1045)
                     /gene="rpmF"
                     /locus_tag="AC16G0_16770"
     CDS             complement(863..1045)
                     /gene="rpmF"
                     /locus_tag="AC16G0_16770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR5101310.1"
                     /translation="MPLPKRRHSNARTAKRRAHDAIQTAGLVRCTHCQAMTLPHRACR
                     SCGYYRGREVVKRSKD"
     gene            complement(1122..1634)
                     /locus_tag="AC16G0_16775"
     CDS             complement(1122..1634)
                     /locus_tag="AC16G0_16775"
                     /inference="COORDINATES: protein motif:HMM:NF014657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR5101311.1"
                     /translation="MLIEVSKIPPEGLDVVLPEEELDLGASAGVWSGPACVRADLHVD
                     RSGRGLLISGAFTGGVPLVCSRCLEGFSYQAEDRFQVYCAVAPQTALEEEHELSDDEL
                     DVTYLEEGRINTDHLLRENILLSLPIQPLCHEDCHGLCPRCGANLNQGACGCTETRVD
                     PRLQVLRKLL"
     gene            complement(1849..2283)
                     /gene="ndk"
                     /locus_tag="AC16G0_16780"
     CDS             complement(1849..2283)
                     /gene="ndk"
                     /locus_tag="AC16G0_16780"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HIR5101312.1"
                     /translation="MERTLVMVKPDAVKHGHVGEVIRRFEAAGLTLLALKMVWLERRE
                     AEGFYQVHRERPFFASLTQFMSSGPAVAMVLEGAGAIATVRTLMGATDPAKAEAGTIR
                     KDLATSVEQNAVHGSDSPGSAAYEIPFFFSSLEQHTYQAGNR"
     gene            complement(2421..>2858)
                     /locus_tag="AC16G0_16785"
     CDS             complement(2421..>2858)
                     /locus_tag="AC16G0_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR5101313.1"
                     /translation="IGTTFVDALRLFQADSETVAILMIGEIGGTAEEDAAAFVKAHVT
                     KPVVGFICGQTAPPGRRMGHAGAIISGGTGTAIEKMTAMRKAGITVVKSPAEMGQAVA
                     RVLPPVRARGKILRPAARVGAKPKSGAKAKPAKRRTAKGVRSR"
BASE COUNT          474 a          996 c          890 g          498 t
ORIGIN      
        1 acccggcgct tgaagcggcg gaacgccggg atcaggagga gcgagcccat gcggctccgc
       61 aggtcggtgc gcagctcctc ccggagcagg tggagcatgg tgtcggccaa tccctccgag
      121 atcttcaggg cgacgttgcc cgtgaagccg tcgcagatga tcacgtcgga gctgccgttg
      181 aagacgtctc ggccctcgac gttgccgata aagttcaatg aggcttcgtc ctccagttcc
      241 ttgaacgcct ccctggtcag ctcgttgccc ttgctttcct cctcgccgat gctgaggaga
      301 cccactcggg gccggctgat cccgaggaca tcgcgggcgt agacgttccc catgatggca
      361 aactgaacca gatgcctggc cttgcagtcg acgttggcgc ccacatccag gaggaccgcc
      421 tgccccgtga gggtgggaac gacgacggcg atggccggcc gttcgacgcc aggcaacgtg
      481 cccaggacca ccagggccgt ggccatcacc gccccggtgt tcccggcgct gacgaaggcc
      541 tcggcctggc tgcgcttgat taattccaac ccgatccgga tggaggagtg tttcttgtgg
      601 cgcagcgccg ccagggggct ctcgcccatc tccacggcct cgggtgcatg gcagacgctc
      661 agggacagcc cctggacgtc atgccggcgc atctggcgcc ggatttcctc ctcggccccg
      721 acgaggacca cggccgcgcc gaactctcgg gccgccgcca cggcgccctc gacattcact
      781 tctggtccgg agtccccgcc catggcatcc agggccaccc ggactgtctc ggtccgcgtc
      841 atgccaggaa cccgtcagtg ctctagtctt tggaccgctt gaccacctca cgcccgcggt
      901 agtacccgca actccggcag gctcgatgcg ggagcgtcat ggcttgacag tgggtgcagc
      961 ggaccagacc ggcggtctga atagcgtcat gcgcccgacg cttggccgtt cgggcattac
     1021 tgtgccgtcg cttggggagc ggcatgtgtc atcctctctc gtctcggatc gtcatccggg
     1081 gatcgggtca cgtcggcgga aggcggcaac cgcgcggatg cctacagcaa cttccgcaat
     1141 acctgcagtc gcgggtccac gcgggtttcc gtgcagccgc acgccccctg attcaggttt
     1201 gcgccgcacc gcgggcacag cccgtgacag tcctcgtggc agaggggttg aatggggagg
     1261 ctgagcagga tgttttcgcg caggaggtga tcggtgttga tccgtccctc ctccagatac
     1321 gtcacatcca gctcatcatc ggagagctcg tgttcctcct ctaacgctgt ctggggtgcg
     1381 accgcgcaat aaacctgaaa acgatcctcg gcctgatagg aaaatccctc gaggcagcgg
     1441 ctgcacacaa ggggaacacc gccggtaaag gccccgctga tcagcaggcc gcgtccggag
     1501 cgatcgacgt gcaggtcagc ccggacacag gcgggaccag accagactcc cgccgacgcc
     1561 cccagatcca actcttcctc cggcaggacg acatccaggc cctccggcgg aatcttcgag
     1621 acttcaatca gcatgatgca acatggtgca cgcgtcacat cctggcggga cctgctcaaa
     1681 agcgggcgta gtatactgac gacctctggg ggtgtcaagc gaaaaccccc cgttctcggg
     1741 gccggcatat gccgaaaacg ccaaggggga cgcggagttg cggacgaggt cccctccgcg
     1801 ctgtcggcgc cttccagtca ctggaagccg ggcgggctgc gccgttgtct accggtttcc
     1861 agcctggtat gtatgctgtt ccagacttga aaagaagaac gggatctcgt aggccgccga
     1921 tccgggcgaa tcggatccgt ggacggcatt ctgctccacg ctcgtggcga ggtcttttcg
     1981 gatcgtgccg gcttcggcct tggccgggtc cgtcgccccc atgagcgtcc gcaccgtggc
     2041 gatcgctccg gccccctcca gaaccatggc caccgcgggg ccagaggaca tgaattgcgt
     2101 gaggctggcg aagaagggcc tctccctgtg gacctggtaa aagccctccg cctcccgccg
     2161 ctccagccag accatcttca gggccaggag cgtgagcccg gcggcctcaa accggcgaat
     2221 cacctccccg acgtgcccgt gcttcaccgc atccggcttc accatcacca gcgtcctttc
     2281 catcatgcct ctggtctcct ctcgcccccg ctgcgcaacg tggacaaaca caaaggagac
     2341 catccgcgga gattccgcag gtggtctccc aaaagatccg gatcactccg attcgcgggg
     2401 cgcaacaccg cgcccgcggt tcaccgagac cggacgccct tggccgtgcg acgcttagcc
     2461 ggcttcgcct tggctccgga tttcggtttg gccccgaccc gggcggcggg gcggaggatc
     2521 ttcccgcggg cccgcacggg tggaagcacc cgggctaccg cctgccccat ctccgccgga
     2581 ctcttcacca ccgtgatgcc ggcctttcgc atggcggtca tcttctcgat tgccgtgccg
     2641 gtgccgccgg agatgatcgc acccgcgtgg cccatgcgcc gccccggtgg cgccgtctgc
     2701 ccgcagatga agccaaccac gggcttggtc acgtgtgcct tcacgaaggc cgccgcgtcc
     2761 tcctcggccg tgcccccgat ctcgccgatc atgaggatgg caaccgtctc ggaatccgcc
     2821 tggaacagcc gcagggcatc cacgaaggtg gtcccgat
//
LOCUS       DBNGZC010000972         7691 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1845297, whole genome shotgun sequence.
ACCESSION   DBNGZC010000972 DBNGZC010000000
VERSION     DBNGZC010000972.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7691)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7691)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7691
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1845297"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1899
                     /gene="asnB"
                     /locus_tag="AC16G0_16790"
     CDS             <1..1899
                     /gene="asnB"
                     /locus_tag="AC16G0_16790"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR5101314.1"
                     /translation="VCGIVGMFLREGAASRDRLEAAMEAVRHRGPDEAGVHCDGPTGL
                     GHRRLSVIDLTEGQQPMADAHGTAWITYNGEIYNFAAIRQELEATGVRFRTHSDTEVL
                     LEAYRAKGRACLEDFRGMFAFALWDATRRELFLARDRLGVKPLYTAETPAGFFFASEI
                     PALFALADIPRVLDPVALQHYLTLQYVPAPWTGFAAIRKLPPAHWLRVREGRVVAEEQ
                     YWDVTPVSAPHGDRREAALRLRELLAQATLLRLVSDVPVGAFLSGGVDSSITVALMAE
                     AMDRPVKTFCVGFPESRLDERRYAAEVAQRYGTDHAEVRADLDVATMLPRLVRQAGSP
                     FADASLVPTYAVAAAGRKEITVALTGDGGDEAFGGYKRYGILGFAGAAQARGLAPAWR
                     GLRKLTLFLERRINSRRQKHYPAGPADLVLEERDPVTQYLILTRIFGAEALEALVTPE
                     IRRAWTAGETSAWLRRRWSQTAEADAVCRWMDLDRTGFLAEDVLPKVDIASMAASLEC
                     RSPFLDHVVVEFAASLPGRFLMDSIRKQGKRILRDAFRDAVPAEVMDRPKMGFTPPVA
                     DWLRREVRPLVTDQLLAPGSYYDVIRRDTVGWLVRQHLDGKGAHGKALWTLLSLRLWW
                     DTFGVSLA"
     gene            1896..3011
                     /locus_tag="AC16G0_16795"
     CDS             1896..3011
                     /locus_tag="AC16G0_16795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5101315.1"
                     /translation="MRIALYELLSTVMAGGIQSTIWELSRELCRRGHRVHLYGGQGPI
                     RYEVAGDFAVWTFPFVRRERFPDLGTRFRALCERLSFARHALGPLSGGQYDVVFIRKP
                     YDMPAALWARRRSGARVVFKSGGTEFLPGYRACARRLDGFLACSRFNAEQIRARTGLL
                     PLVHYNGVNPELFRPLPRDEALAASLGIGSEEFVALSVVRLVGWKGIQVAIQAMHALR
                     PQGRYRLVVVGDGDYRTKLEAQVRELGLQGVVTLLGAVPRTEVPRYYSLAGAAVFPSI
                     GDDAFPNSVVEAMACGVPVVATPSGGIPEAVVDGETGLLVPPRDSEGVARALMRLASD
                     PSSLRSMGEKGRLTAVERFDWSRLTDDLLAVFEGRAA"
     gene            3008..3217
                     /locus_tag="AC16G0_16800"
     CDS             3008..3217
                     /locus_tag="AC16G0_16800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101316.1"
                     /translation="MRIPSMPPRDPERIVPVSSDVPVRFDSLRHPRTSTRLAYLRARI
                     AGRLAPAGYGGATFKPVARKPKGPG"
     gene            3343..4119
                     /locus_tag="AC16G0_16805"
     CDS             3343..4119
                     /locus_tag="AC16G0_16805"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5101317.1"
                     /translation="MTVIEIDGWRVVACPACRLGFLDPPLSAEEVAALYDESFYQENR
                     MVAEPDAVQAAIDRQRPRVRYIQRYCRNGRLLEIGAGMGYLLAAARDAGFEVSGLELS
                     PWAAGQARDVLGLEVAVGGVESVEFPPAAFEVIVMWHVIEHFVDPLANLRRLRRWLRP
                     EGILILETRNFMGFDARHLGAAWNGWSLPHHLWHFSPRSLHRMLQAAGFRRTHTRTDH
                     SGFVKERLRRIPVLSALRNVLSYWIPGSNVRAIGFVGEDR"
     gene            4116..5045
                     /locus_tag="AC16G0_16810"
     CDS             4116..5045
                     /locus_tag="AC16G0_16810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5101318.1"
                     /translation="MTSETGLPLICPACGAGLPAASRWNTATIACSGCTACYAAVNGI
                     LRFVPHAGYSQSFGFQWVRHARTLMDDATRSGSEQTLRRMLVTPDLVKGRRVLDAGCG
                     VGRFADVLLRWGAEVVAVDLSDSVDVAQANLRGRGGLTIAQADILRLPFPPGTFDAIV
                     SWGVLHHTTDCAAAFRALVRLLRPGGTMAIWVYGKSRSVRRRVMDAYRHVTVHLPHRL
                     LYALCFVAIPMYYVYKVPILGNLLRVVVPMSRQRSARERVLETFDYYSPRYQSKHTFP
                     EVHGWFVEAGFRDIRIFNPPVTVIGRRPMPGDG"
     gene            5099..6271
                     /locus_tag="AC16G0_16815"
     CDS             5099..6271
                     /locus_tag="AC16G0_16815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIR5101319.1"
                     /translation="MAGGASLLSRGLEFLLEWGLHRRMPGPPPPFETGRIQRILVVRK
                     DNIGDLVCTTPALRALRRAFPAAHLAILVPKHCLPVVQRNPDVDEVLTYTKAKHQSGA
                     QGLPALWSLAQLLRDLRRRRFDLAISMRRSYSQSSAWLAYATGAAWRLGYPAPATHPC
                     QFFLNLGPDGLGLTQHEVDACLELLASVGIPAAGRELTLVPDPDARAAIGRSLKEAGV
                     PQGSALALVHISSRREASRWPLAFFARAADLLRERLGLCILLSWSPGSGTSALFPGDD
                     GQAEEVAGQMATRPVFLRTPTLNDLIAALSLCDLVLSTDGGLMHMAAALDVPQVALMG
                     RSDPRQWAPVSRKCVALHRDGRADRVSVDEVVTAAADLMARWGRETASGLDSRARP"
     gene            6356..7537
                     /locus_tag="AC16G0_16820"
     CDS             6356..7537
                     /locus_tag="AC16G0_16820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5101320.1"
                     /translation="MTGCLRVAMVSLDFRREGGSEGRTGQLVDRLIAEGHEVHLVGAR
                     IRGPWDARTVLRQVPTPAHPHWLEVMLFCRRARALVAREGFDIVHNQIRPFVPGVVTA
                     GGGCHRFYVQEVLPREKGRVRAGLKRVSPLHLVLLGLERRGFRTVSCPYIIANSCLSR
                     DGILKYYPMPPDRVVVAYNGVDPVRFSPARRDLLREQRRAALGVRPQEFLVLFVGAGF
                     ARKGLGPLLAAVSRLQSRGPRPRLVVVGGGRSGPWHRHAMRLGLADRVSFVGAVSDPE
                     SYYAAADVVALPTFFDPFANVTLEAMASGLPMVTSRHNGAAEILRQGVDGLVVDCAED
                     VNGLADALAWLQDPLQRAAMGEHARETALKYPWEGPLDKTLEVYRAVMKRGRGAEAQG
                     G"
BASE COUNT         1091 a         2432 c         2808 g         1360 t
ORIGIN      
        1 gtgtgcggga tcgtggggat gtttctgcgg gaaggcgccg cgagtcggga tcgcctggag
       61 gcggcgatgg aggccgtccg tcatcgcggc ccggacgagg ccggcgtcca ttgcgacggt
      121 cccaccgggc tcggacaccg gcgcctctcc gtcatcgacc tgaccgaagg gcagcagccc
      181 atggccgatg cccatggcac cgcctggatc acgtacaatg gtgagatcta caactttgcc
      241 gcgatccggc aagagctgga ggcgaccggc gtccgcttcc gcacccacag cgacacggaa
      301 gtcctcctgg aagcgtatcg agccaagggc cgcgcttgcc tggaggactt ccgtgggatg
      361 ttcgccttcg ccctgtggga cgcgacgcgc cgggagctat tcttggcccg tgaccgcttg
      421 ggggtgaagc ccctgtacac ggcggagacc ccggcgggct tcttcttcgc ctccgagatc
      481 ccggcactct tcgccctggc cgacattccc cgggtgctgg acccggtggc gctccagcac
      541 tacctcacgc tccagtacgt gccggcgccc tggaccgggt ttgcggcgat ccggaagctc
      601 ccgcctgctc actggctgcg tgtccgggag ggccgcgtcg tcgcggagga acagtactgg
      661 gatgtcacgc cggtgtccgc tccccacggc gatcggaggg aggcggcgct ccgcctgcgg
      721 gagcttctcg cccaggctac gctgctgcgc ctggtcagcg acgttcccgt gggcgcgttt
      781 ctcagcggcg gcgtggactc cagcatcacc gtggccttga tggcagaggc catggaccgg
      841 cccgtgaaga cgttttgcgt cggcttcccg gaatcccggc tggacgagcg ccgctacgcc
      901 gccgaggtgg cgcagcggta cgggaccgat cacgcggagg tcagggcgga cctggacgtg
      961 gcgaccatgc tgccgcggct cgtgcggcag gccggctccc ccttcgcgga tgcgtccctg
     1021 gtcccgacct atgccgtggc ggccgcggga cggaaggaga tcaccgttgc cctgaccggc
     1081 gacggcgggg acgaggcctt tggaggctac aagcgctacg gcatccttgg gtttgcaggg
     1141 gccgcgcagg ccaggggcct ggccccggcc tggcgagggc tgcgcaagct cacgctgttt
     1201 ctggagcggc ggatcaactc ccgacgccag aagcactacc cggccggccc ggccgacctc
     1261 gtgctggagg agcgggaccc agtgacgcag tacctgatcc tcacgcggat cttcggggcc
     1321 gaggcgctgg aggccctggt gacgccggag atccgccggg cctggacggc cggcgagaca
     1381 tccgcgtggc tccgccgccg ctggagccag acagccgagg cagacgccgt gtgccgatgg
     1441 atggatctgg accggacggg atttctggcc gaggacgtgt tgcccaaggt ggatatcgcc
     1501 agcatggcgg cctccttgga atgccggtct cccttcctgg accatgtcgt ggtggagttc
     1561 gccgccagcc tgccggggcg gttcctcatg gattcgatcc ggaagcaagg gaagcggatc
     1621 ttgcgggatg ccttcaggga tgcggtcccg gcggaggtga tggaccggcc caagatgggc
     1681 ttcacgccgc cggtggcgga ttggctgcgc cgagaggtcc ggccgcttgt gacggatcag
     1741 ctcctggcgc cgggatccta ttacgatgtc atccggcggg atacggtggg gtggctcgtg
     1801 cgccagcacc tcgacgggaa gggagcgcac gggaaggcgc tctggaccct gctcagcctg
     1861 cgcctctggt gggacacctt cggcgtgagc ttggcatgag gatcgccctg tacgaactcc
     1921 tctcgaccgt gatggccggg gggatccaga gcaccatctg ggagctgagc cgggagctgt
     1981 gccgtcgcgg gcaccgggtg catctgtacg gcggccaggg gccgattcgg tacgaagtgg
     2041 ctggggactt cgcggtttgg acattcccct ttgtccggcg ggaacggttt ccggatctgg
     2101 ggacccggtt ccgcgccctg tgtgagcggc tgtcctttgc ccgtcacgcg ctagggccgc
     2161 tctccggtgg gcagtacgac gtggtcttca tccgcaagcc ctacgacatg ccggccgcgc
     2221 tctgggccag gcggcgctcg ggggcccgcg tggtgttcaa gagcgggggg accgagttcc
     2281 tgccggggta tcgcgcctgc gccaggcggc tggacgggtt cctggcgtgc agccggttca
     2341 acgccgagca gatccgagcc cggacgggcc tgctgcccct ggtccattac aacggggtca
     2401 atccggaact attccgaccg ctgcccaggg acgaagcgct ggcggcttcc ctggggatcg
     2461 gctcggagga attcgtggcc ttgtcggtcg tccgccttgt gggctggaag gggatccagg
     2521 tggccattca ggcgatgcac gccctccggc cccagggtcg atatcgcctt gtggtcgtgg
     2581 gggatggcga ctaccggacc aagctggagg cccaggtgcg ggagttggga ctccagggcg
     2641 tggtgaccct cctgggggcc gtgccgcgta ccgaggtgcc acgctactac agcttggccg
     2701 gggccgcggt ctttccgagc atcggggacg atgcctttcc caactcggtg gtggaggcca
     2761 tggcctgcgg cgtgccggtc gtggccacgc cctcgggcgg catccccgaa gcggtggtgg
     2821 acggggagac ggggctcctg gtcccgccgc gcgactcgga aggagtggct agggccctga
     2881 tgcgcctcgc atccgatccc tccagcttgc gatccatggg ggaaaaagga cggttgacag
     2941 cggtggagcg attcgactgg agccgcctca ccgatgacct cctcgccgtg tttgagggga
     3001 gggcggcatg agaataccgt cgatgcctcc gcgcgacccc gagcggatcg ttcccgtatc
     3061 gtccgacgtg ccggtgcgat tcgatagtct tcgccatccg agaacatcca cgcggctggc
     3121 atacctgcgc gcccgcatcg ccggcaggct cgccccggcc gggtatggcg gcgcgacctt
     3181 caagccggtg gcacgaaagc caaagggtcc aggatgaggt ccggcggctc catggaatct
     3241 cctggaccct ccatccgtcc accctccgga gacgggatgc ttatgccacc agcggcgaga
     3301 gcctctcgca gcgtgtgccc gctgtgcgga ggagtcgctc gggtgacggt gattgaaatc
     3361 gatggctgga gagtggtagc gtgtccggcc tgtcgtctgg ggttcctgga tccgccgctc
     3421 tcggccgagg aggtggccgc actctacgat gagtcgttct accaggagaa ccggatggtg
     3481 gccgagccag atgccgttca agccgccatc gacaggcagc ggccgagggt gcggtacatc
     3541 caaagatatt gccggaacgg tcgcctcctg gaaatcggag ccgggatggg gtacctgctc
     3601 gccgcggcca gggacgccgg gttcgaggtg agtggcctgg agttgtcccc ctgggccgcc
     3661 gggcaggccc gggacgtgct cggtctggag gtcgcggtgg gcggggtgga gtcggtggag
     3721 tttcctccgg ccgccttcga ggtgatcgtg atgtggcacg tcatcgaaca cttcgtggat
     3781 ccactggcca acctgcgtcg cctgcggagg tggcttcgcc ccgaagggat cttgatcctg
     3841 gagaccagaa acttcatggg cttcgatgcg cgacatctgg gggcagcatg gaacggctgg
     3901 tccctgcccc atcatctgtg gcacttcagt ccccgcagcc tgcatcggat gctgcaggcg
     3961 gcaggttttc ggcggaccca tacgcgcacg gatcattccg gattcgtcaa ggagcggctc
     4021 cgccgtatcc cggttctctc ggccctgcgc aacgtccttt cgtattggat tccgggctcg
     4081 aacgtgcggg ccatcggctt tgtgggggaa gaccggtgac gagcgaaacg gggctgccgc
     4141 ttatttgccc tgcctgcggc gcgggactgc cagctgcgtc gaggtggaac acggcgacca
     4201 ttgcctgctc gggatgcacc gcctgctacg cagccgtcaa cggcatcctc cggttcgtcc
     4261 cgcatgcagg ctattcgcaa agttttggct ttcagtgggt gcgccacgcc cgaacgctga
     4321 tggatgacgc gacacgcagc ggatccgaac agaccctgcg tcgaatgctg gtcacgcccg
     4381 atctggtgaa ggggcgtcga gtcttggacg ccggctgcgg ggtgggtcgc tttgccgacg
     4441 tcctgttgcg ctggggtgcc gaggtcgtgg cggtggatct gagcgattcc gtggacgtgg
     4501 cccaggcgaa cctccggggc cgcggggggc tgaccattgc ccaggcggat attctgcggc
     4561 tcccctttcc tccggggacg ttcgacgcga tcgtgtcctg gggggtcctg catcacacga
     4621 cggattgcgc cgcggccttc cgggcactcg ttcgtctgtt gcggcccgga ggaacgatgg
     4681 ctatctgggt ctacggcaag tcccggagcg tccggcggcg ggtcatggat gcgtatcggc
     4741 acgtcacggt gcacctgccg caccgcctgc tgtatgccct ctgcttcgtg gcgatcccga
     4801 tgtattatgt gtacaaggtt ccgatcctgg gcaatctgct gcgcgtcgtg gtccccatga
     4861 gccggcagcg cagcgcccgt gaacgggtgc tggaaacctt cgattactat tcgccgcggt
     4921 accagtccaa gcacaccttc cccgaggtcc atggctggtt cgtggaggcc gggttcaggg
     4981 atatccgaat cttcaacccg ccggtcacgg tgatcgggcg acgaccgatg cccggggatg
     5041 gctgacgggc tggctgcggg ccgacggcca gggccgagcg catcccgagg acaagacgat
     5101 ggccggtggc gcgagcctgc tgagccgtgg actggaattc ctcttggagt ggggcctgca
     5161 ccggaggatg ccgggtcccc cgcccccctt cgagacgggg cgtattcaac gcatcctcgt
     5221 cgtccgcaag gataacatcg gggacctggt gtgcacgaca ccggcgcttc gcgccctgcg
     5281 gcgggccttc cccgctgccc atctggccat cctggtgcct aagcactgcc tgccggtcgt
     5341 gcagcggaat ccggacgtgg acgaggttct cacctacacg aaagccaagc accaatccgg
     5401 tgcgcagggt ctgcctgcgc tgtggagtct ggcgcagctc cttcgcgacc tccgacggcg
     5461 acggttcgac ttggccatct ccatgcgccg ttcctacagc caatccagcg cctggctcgc
     5521 ctatgccacc ggggcagcct ggcggctcgg ctatccggcc ccggcgacgc acccgtgtca
     5581 attctttctg aacctgggac cggacgggct cggcctgacg cagcacgagg tggacgcctg
     5641 cttggagctg ctggcctcgg tcggcattcc agcggcgggg cgagagctga ccctcgtccc
     5701 cgatcccgat gcccgggccg cgatcggccg gagtctgaag gaagcggggg tccctcaggg
     5761 gagcgcgctg gcgctcgtgc acatcagcag ccgtcgggag gccagccgat ggcccctggc
     5821 gttcttcgcc cgagccgccg atctcttgcg tgaacgtctg gggctctgca tcctgctctc
     5881 ctggtctccc gggagtggta cgagtgctct gttcccgggg gacgacggcc aggctgaaga
     5941 ggtggcgggg cagatggcga cgcgaccggt attcctccgg accccgaccc tgaacgatct
     6001 gatcgccgcc ctgagtctct gcgatctggt cctctccacg gacgggggcc tgatgcacat
     6061 ggccgccgcg ctggacgtgc cccaggtggc tctcatggga cggtcggacc cgcgccagtg
     6121 ggcgccagtc agccggaagt gcgtggccct gcatcgggac ggtcgggcgg atcgggtctc
     6181 ggtggatgag gtggtgactg ccgccgcgga tctgatggcc cgctggggtc gggagaccgc
     6241 atccggactg gattcgaggg cgaggccgtg agggatgtcc ggtgagttgt gggcgaatcc
     6301 gaggccaggg ctggctcatc cggggaagca gcctgtgtca cccgcggggt cggccgtgac
     6361 cggctgcttg cgcgtggcca tggtgtccct ggatttccgt cgagaggggg ggagcgaggg
     6421 gcgcacgggg cagctggtgg atcgtttgat tgccgagggg catgaggtcc atctggtggg
     6481 ggcccgcatc cgcgggccgt gggatgcgcg gacggtcctg cggcaggtgc ccacgcctgc
     6541 ccacccgcac tggctggagg tgatgctctt ctgtcgacga gcccgagcat tggtcgcgcg
     6601 tgaaggcttc gacatcgtcc acaaccagat ccggcccttc gtccccgggg tggtcactgc
     6661 ggggggcggg tgccatcggt tctacgtgca agaggtcctc ccacgcgaga agggccgggt
     6721 gagggcggga ctgaagcggg tgtccccgtt gcatctcgtc ctgctgggcc tggagcggag
     6781 gggcttccgg accgtgagtt gtccgtacat catcgcgaac tcgtgtctga gccgggacgg
     6841 gatcctcaag tattacccca tgccccccga ccgggtggtg gtggcctaca acggggtgga
     6901 cccggtgcgt ttcagtcccg cccggcggga ccttcttcgc gaacagcggc gggcggcgct
     6961 cggggtcaga ccccaggaat tcctggtgct cttcgtgggc gccggcttcg cccggaaggg
     7021 actggggccc ctcctggcgg ccgtgtcgag gcttcagagc cgcgggcccc gaccacggct
     7081 ggtcgtggtg ggtggcgggc gaagcgggcc gtggcataga cacgccatgc gtcttggtct
     7141 cgccgatcgg gtatccttcg tgggtgccgt ttccgacccg gagagctact atgcggctgc
     7201 ggacgtggtg gcgctgccaa ccttcttcga tcccttcgcc aacgtcacgc tggaagccat
     7261 ggcgtccggt ctgccgatgg tcacctcacg tcacaacggg gcggccgaga tcctgcgcca
     7321 gggtgtggac gggctagttg tggattgtgc cgaggatgtg aatggcctgg ccgacgccct
     7381 ggcctggctc caggatccgc tgcagagggc cgccatgggc gagcacgcac gcgagacggc
     7441 gttgaagtat ccctgggaag gtccgttaga caagaccctg gaggtgtacc gggcggtgat
     7501 gaagcgaggt agaggcgctg aggcgcaggg gggctgaggc gcatgggcgt tcgcctcagc
     7561 gccccagcca gatagtgcag gagagccgga gatggatagc cttgcagtgg cgaggcgggt
     7621 gctggagatc gagggggagg ccatccgggc gttgatcccg cggctggggg aggccttcat
     7681 ccgcgccgtg g
//
LOCUS       DBNGZC010000973         1831 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1744150, whole genome shotgun sequence.
ACCESSION   DBNGZC010000973 DBNGZC010000000
VERSION     DBNGZC010000973.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1744150"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="AC16G0_16825"
     CDS             complement(<1..647)
                     /locus_tag="AC16G0_16825"
                     /inference="COORDINATES: protein motif:HMM:NF012209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase/acyltransferase domain-containing
                     protein"
                     /protein_id="HIR5101321.1"
                     /translation="MSIAELFFAKQAQRTQLFRIPRRTKVPRGRRPLPPRHQKFLLEP
                     LEPRLLLSGSGILKPVLVIPGFGGTMPAAVTDAGLEEWLTTRGISPDKLQVDPLANVY
                     DDLIQTLENVGYVEDDTLFVANWDWRVPVAPRDGVRDGGLSNVSAAAITDATYQTGLD
                     YLGYWLKQAQDAWFASHGEPLTSVDIIAHSTGGLVARSYIQSEAYGDQYDSNGNGT"
BASE COUNT          356 a          594 c          562 g          319 t
ORIGIN      
        1 gtaccattgc cattggagtc gtactggtcg ccataggctt cgctctggat gtagctgcgt
       61 gcgacgagtc cgcccgtgct gtgggcaatg atgtcaacgg aggtaagagg ttctccatgc
      121 gacgcgaacc atgcgtcctg cgcctgcttg agccagtagc ccagatagtc gagccccgtc
      181 tggtacgtcg cgtcggtgat ggcggcggcg ctgacattcg acaggccacc gtctcgcact
      241 ccgtcccggg gcgcgaccgg cacccgccag tcccagttag ccacgaagag tgtatcgtcc
      301 tcgacgtagc ccacgttctc gagggtctga atcaggtcat cgtagacgtt tgccagagga
      361 tcgacctgga gcttgtcagg cgatataccc cgggtggtaa gccactcttc cagtccagca
      421 tcggttacgg cggcaggcat ggtcccgccg aatccgggaa tgaccagcac cggcttaagg
      481 attcctgagc cgctaagcag caagcgcggc tcgagcggct cgaggaggaa cttttggtgc
      541 cgaggcggga gcgggcgccg gccacgcggg accttggttc ggcgcggaat gcgaaacaac
      601 tgggtccgct gggcctgctt ggcgaagaac aattcggcga tgctcatcgt catcctcctg
      661 cggtcgaggg aacactacgt atgattgcgt gtgatgaaac gacacgacgc cccggctgga
      721 ccgcgagcac catgcgcagt ccacaggggg ttataacgcc tgtcctgccc tgcaacctgg
      781 atcctttcgg gaatgcgaca ccccgcctag gacacaaact ggcgggcgag ggacaacgcc
      841 ccgacgagcg agccaggctg tgcccgtctc gcccatccgg ctccttgccg gtcatcgcct
      901 ggtcccatac aaacacaatg gccgagcacc ggggcgtcct tcagatcctg ccgccagggc
      961 tcggccacgt tgagcgaatg gaagaagagc gaacgatcag tggctaaggc gctgcgcgcc
     1021 cggtggtgcg ccacgaggcg agtccccgcc gtcagaccaa agcccgggaa cggattgagg
     1081 gaggcggatt cttggagagg cagaaccgcg gccgaagtca aggcgtctcc cgcagccgtg
     1141 cagtcctcgg ccccaatcgg ccagagcgca acgtccgcgt cgaacgcctc aagcaagaca
     1201 aatgcaccaa gtgcggccac cagacaggtt agggtcacac gaagaccctt gccccgcaag
     1261 aacctacgcc ggtcctccat ggtacgacga aaatgaaagg ggatgccaga cagaggcggg
     1321 ccgttgagtt gcccgggccc aggtccccgg gttgcgtcca actccacggg tctgacccta
     1381 cacatggccc cgcttcgaga ggactaacag gagctttccg agtcagaggg tgcctggctc
     1441 agtcatgcgg gtagatcgat aggtcaaccc tactcttttc gtttgccacg tcaacagaaa
     1501 attcagtcgt gtgtgagaaa ctcctacgag aatcatcccc atttcggttg cccagggcac
     1561 gctgtctcac cccggcaatt gcatcgaggg cccctccggc cgggttcccc gcgcgatcgc
     1621 cccaatttgc ttcctccaca ctgctctcac gcccattccg attaccccct tgcccgcagg
     1681 tctgctcgaa acgagaactg ccgccggggc tgacccggcg acagccgtgg tttcgtcatg
     1741 agcgccctgg tctcacatct agccgctggt gctcggaggc cccggcacgc cgagggcacc
     1801 gaaccgggtc tcgtactcca gcagcacgtt g
//
LOCUS       DBNGZC010000974         4177 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1549357, whole genome shotgun sequence.
ACCESSION   DBNGZC010000974 DBNGZC010000000
VERSION     DBNGZC010000974.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1549357"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1853
                     /locus_tag="AC16G0_16830"
     CDS             <1..1853
                     /locus_tag="AC16G0_16830"
                     /inference="COORDINATES: protein motif:HMM:NF024754.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR5101322.1"
                     /translation="SVFASDEAEDLSRVLLAAAEPGREALVRSALVTDLLGLTGDALE
                     ALTADEAAWERRLEAFQGDHERWQDQGFVRMMRELLRREGVPRRLLRFPDGERRLTNL
                     LHLVELLQAEEDAEGLGMTGLIHWLVDRRSAGTEGAEEAQLRLESDENLVKIATIHKS
                     KGLEYPIVFCPFLWDGRLVSADEETLRYHDPTANHRPTLDLGSLHQAEARAQARREEL
                     AENLRITYVALTRAKHRCYAVWGHVKAGATSPLAYLLHQPPHLGNDPMDAVETHMGTL
                     GSEEIRADLDSLAQVSGGTIAVRPISQEAPNIFRSPTTAPEELQARVFTGSLAVPWRI
                     ASFSALARESEAAVEWLDDDLGVLAQPLAPSTGALDISRFPRGPQAGLCLHALFERVD
                     FAGSKTTEETLAGETLAAHGFDPVWAPVVAGMLGRVLDCPLDPWEAPLRLRDIGRGDR
                     LDELEFHYPVARVTDAGLKRLLRAHGYGAGTRIQDEVERLTFAPVEGYMRGFIDLIVR
                     RGERFYLVDYKSNWLGEDLEAYRADRLSAVMAQEAYYLQSLIYLVALHRYLGHRLPGY
                     TYERHLGGIYYLFLRGMDPGHGPEAGVYRDRPGEALVLALDRYLATGEGA"
     gene            1861..3684
                     /gene="recD"
                     /locus_tag="AC16G0_16835"
     CDS             1861..3684
                     /gene="recD"
                     /locus_tag="AC16G0_16835"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HIR5101323.1"
                     /translation="MLDRLEALRRGDVFEDLDLHFARLISRVGDGEGSVALAAALASQ
                     WTGRGHICLDLASVAGTRVGEEVGEGVIEAPALERWVEQLRRCPAVGHPGAFTPLVLD
                     EAGRLYLYRYWDYQREVADALAHRLQDNPTDVDLPRLREGLDRLFTLSADGPDWQRIA
                     AAAAVLRRFTVISGGPGTGKTTTVIRLLALLVEQGSGRPLRIGLAAPTGKAAARMQEV
                     IRAAKRDLPVEDSVRDVIPETAATIHRLLGWRPGTVGFRHDRENPLGLDVLVVDEASM
                     VDLALMAKLIRALPQAARLVLVGDRDQLASVEAGAVLGDICGDSPGFSAAFRRRLREA
                     TGTDPGGEGDGPPIRDAVVLLQRSYRFGERSGIGQAARLVNQGEGRQAFELLEGGNFP
                     DVVWRDVETPGELRRALAATAADAFRDAASVDEPEAVLAALDGFRILCAHRSGPFGLE
                     VVNRLVEERLDAAHLISPRGAWYRGRPILVTTNDYHLHLFNGDLGVALPDPEAGQILR
                     IFFRAAEGGLRRIPPARLPVHETAYAATVHKSQGSEAERVLLILPNEASPVMTRELIY
                     TGLTRARSRVEVWGTPSVFETAVTRRLTRSSGLRDALWRSQ"
     gene            complement(3945..4148)
                     /locus_tag="AC16G0_16840"
     CDS             complement(3945..4148)
                     /locus_tag="AC16G0_16840"
                     /inference="COORDINATES: protein motif:HMM:NF021560.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HIR5101324.1"
                     /translation="MRVKYFADTDTALVEFTENKVAQTKEISENIYVDLDNQGNLVSM
                     TIEHARTNARLQEFSYQEVAGRT"
BASE COUNT          634 a         1383 c         1473 g          687 t
ORIGIN      
        1 acagcgtctt cgcctcggac gaagccgagg acctctcgcg ggtcctcctg gccgcggcgg
       61 agccgggccg ggaggcgctg gtccggtcgg ccctggtgac cgacctcttg ggtctcacgg
      121 gggatgccct ggaagccctg acggcagatg aggcggcctg ggagcggcgg ctggaggctt
      181 tccagggaga ccacgagcgc tggcaggacc agggctttgt ccggatgatg cgcgagttgc
      241 tccgccgcga gggggtgccg cgccgtctcc tcaggttccc ggacggggag cgccgcctca
      301 ccaacctgct ccacctcgtg gagctgctcc aggctgaaga ggatgcggag ggcctgggga
      361 tgaccggcct gatccactgg ctggtggacc ggcgaagcgc cggcaccgag ggggcggagg
      421 aggcgcagct tcgtctggag agcgacgaga acctggtgaa gatcgccacc atccacaaga
      481 gcaagggtct ggagtacccc atcgtgttct gccccttcct gtgggacggc cggcttgtgt
      541 ccgcggacga ggagacgctc cgctatcacg accccacggc caatcatcgg cccacgctgg
      601 acctgggttc cctacaccag gcagaggcgc gggcgcaggc ccggcgggag gagctggccg
      661 aaaatctgcg catcacgtac gtggccctca cccgggccaa gcaccgctgc tatgcggtct
      721 ggggtcacgt gaaggctgga gcgacgtcgc cgctcgcgta tctcctgcac cagccccccc
      781 acctcggcaa cgaccccatg gatgcggtcg aaacccacat gggaaccctc ggctccgaag
      841 agatccgggc ggacctggac agcctggcac aggtgtccgg gggcacgatc gcggtgcgac
      901 ccatttccca ggaggcaccg aatatcttcc ggtcgcccac gacggcacct gaggaattac
      961 aggctcgcgt cttcacaggg agcctggccg tgccctggcg gatcgcaagc ttctccgccc
     1021 ttgcgcggga gtccgaagcc gcggtggagt ggctggacga cgacctcggg gtcctagccc
     1081 aaccgctcgc gccgtcgacg ggggcgctcg acatcagccg gtttccgcgg gggccacagg
     1141 ccggcctgtg cctccacgcg ctgttcgagc gggtggattt cgcagggtcc aagacgaccg
     1201 aagagacgct ggcgggggag acgctggcgg cccacggatt tgacccggtg tgggccccgg
     1261 tcgtggccgg gatgctgggg cgcgtgctcg actgtccgct cgacccctgg gaggcgccgc
     1321 tccgcctccg ggatatcggg cgaggcgacc gcctggacga gttggaattc cattaccctg
     1381 tggctcgggt caccgacgcg ggtctgaagc gattgctccg ggcgcacggc tacggggcgg
     1441 gcacgcggat ccaggacgag gtggagcggc tcacgttcgc gcctgtcgag ggttacatgc
     1501 gggggttcat agacctcatc gtccggcggg gcgagcggtt ctacctcgtg gactacaagt
     1561 ccaactggct cggcgaggac ctcgaggcct accgcgcgga tcgcctgtct gcggtcatgg
     1621 cccaggaggc ttactacctt cagtccctga tctacctggt ggcgctccac cggtatctcg
     1681 gccatcggct gccggggtat acgtacgagc ggcacctggg cggcatctac tacctgttcc
     1741 tccgcgggat ggatccgggt cacggcccag aggccggcgt gtaccgggat cggcccggcg
     1801 aggcgctggt cctcgccctg gacaggtacc tcgcgaccgg cgagggggcg tgaggtgagc
     1861 atgctcgaca gactagaggc actgcggcga ggcgacgtat tcgaggatct cgacctccac
     1921 tttgcccggt tgatctctcg ggtgggggac ggggaaggct cggtcgccct cgccgccgcg
     1981 ctggccagcc agtggaccgg gcggggacac atctgcttgg acctcgcaag cgtggcaggg
     2041 acccgcgtgg gcgaggaggt gggggagggg gtcatcgagg ccccagccct cgaacggtgg
     2101 gtcgagcagt tgcgccggtg tcccgccgtc gggcaccccg gcgcgttcac gccgctcgtc
     2161 ctggatgagg cggggcgcct gtacctctac cggtactggg actaccagcg cgaggtggcc
     2221 gacgcgctcg cccaccgcct ccaggacaat ccgacggacg tggacctgcc gcgcctccgc
     2281 gaagggctgg accggctgtt cacgctgtcg gcggacggac ccgactggca acgcatcgcc
     2341 gccgccgccg ctgtcctccg gcgcttcacg gttatctcgg gagggccggg gacgggcaag
     2401 accacgacgg tgatccggct cctggcgctg cttgtggagc agggctccgg gcggccgctc
     2461 cggatcggcc tcgctgcccc caccggaaaa gccgccgcgc gcatgcagga ggtgatccgc
     2521 gcggccaagc gcgacctgcc cgtggaggac tccgtccggg atgtcatccc cgagacagcc
     2581 gccacgattc accggttgct cgggtggcgg ccaggaacgg tcggcttccg gcacgaccgc
     2641 gagaacccac tcgggctgga cgtcctggtc gtggacgagg cctcgatggt ggacctggcc
     2701 ctcatggcca agctgatccg ggcgcttccc caagcggcca ggctggtgct ggtgggcgac
     2761 cgggaccagc tcgcgtcggt cgaggcggga gccgtcctgg gtgatatctg cggggattcc
     2821 cccgggttct ccgcggcctt tcgcaggcgt ctccgcgagg ctaccggcac ggatccgggc
     2881 ggcgagggcg acggcccacc catccgggac gccgtcgtgc tgctgcaacg gagctatcgc
     2941 tttggagagc gaagcggcat cgggcaagcg gcccgcctgg tgaaccaggg ggaggggagg
     3001 caggcgttcg agctgctgga gggaggcaat tttcccgatg tagtttggcg ggacgtggag
     3061 acaccagggg agctacgccg agcgctggcc gcgaccgcgg cagatgcgtt ccgggacgcg
     3121 gcgtccgtcg acgagcccga agcggtgttg gccgcgctcg atgggttccg catcctctgc
     3181 gcccaccgga gcggcccctt cggcctggag gtggtaaatc ggctcgtcga ggagcggttg
     3241 gatgcagcgc atctcatcag cccacggggc gcctggtacc ggggccgacc catccttgtc
     3301 accaccaacg attatcacct ccacctcttc aacggggacc tgggcgtggc actccccgac
     3361 cccgaagcgg gtcagatcct tcgcatcttc ttccgcgcgg ccgaaggggg tctccgccgc
     3421 attccgcccg cccggcttcc cgttcacgag accgcctacg ccgccacggt ccacaagagc
     3481 cagggttcgg aggcggagcg ggtcctgctg atcctgccca acgaggcgag cccggtgatg
     3541 acccgggaac tgatctacac cggcctcacg cgagcccgct cccgcgtcga ggtctggggc
     3601 accccttccg tcttcgagac tgccgtcacc cgccgcctga cccgctcctc gggcctgcgg
     3661 gatgcgctgt ggcggtcgca atgagctggg accctcgagg attcgagaga aggggacccg
     3721 gcgcccatcg gtcatccgtg gattcgcagc cagggtaggg atccggtggc tcacgcgcgt
     3781 gcggatcacc gggacgcggc agggacgctt cgcgccccat atccgtaatg tgcttcagaa
     3841 cccacgcagt attaggtcag agcgacttcg tgcaggcaac aacccatttc agcgaaagcg
     3901 gagccggagt tggtccgcta tgcagcgcct tattcggtct acccctacgt ccggcctgca
     3961 acttcttgat acgagaactc ctgtagccgt gcattcgttc gggcatgctc gatcgtcatg
     4021 cttacgaggt tcccttgatt gtcgaggtca acgtatatgt tctcgctgat ctccttcgtt
     4081 tgggcgacct tgttttcggt aaactcgacg agagcggtat cggtgtcggc gaaatactta
     4141 actctcattg ggggttctcc ttgtacgacc tatcgaa
//
LOCUS       DBNGZC010000975         4242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1057879, whole genome shotgun sequence.
ACCESSION   DBNGZC010000975 DBNGZC010000000
VERSION     DBNGZC010000975.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1057879"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="AC16G0_16845"
     CDS             <1..729
                     /locus_tag="AC16G0_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HIR5101325.1"
                     /translation="THTAIHAVKPDHVATFLGRKLTGRYQDELGNDFSTRIQGTRIKH
                     HMGWAAIKLYDKFGLIARVECTANDVSFFQHHRTVEHRDGTKEFKLASVRKSIYSLPV
                     LRELLGAATFRYLDFLAALEDPRPGLRALEKIATPVHAGERSYRGFNLFHGPDLDLFR
                     TILRGEFTISGFQARQLRRHMADCSAGQLSRGLKRLRLHGLIKKIGKRYKYYLTTLGR
                     TVATAALKLRELVIIPMLNQPAPA"
     gene            1259..3862
                     /locus_tag="AC16G0_16850"
                     /pseudo
     CDS             1259..3862
                     /locus_tag="AC16G0_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004358198.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
     gene            3855..>4242
                     /locus_tag="AC16G0_16855"
     CDS             3855..>4242
                     /locus_tag="AC16G0_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101326.1"
                     /translation="MGKSRNKPTQAADLRRRAEKGLKAREPDADLQRSAAETQRLLHE
                     LQVHQMELKLQNEELLQVRDALELRVKEKTAEVVRRTAAEQEVREQSEQLRSLAAELT
                     LAEQRERQRLASVLHDEHQQLLVGAKL"
BASE COUNT          793 a         1431 c         1343 g          675 t
ORIGIN      
        1 acccacacgg cgatccacgc cgtgaagccc gaccacgtgg ccacgttcct cggccgcaag
       61 ctcaccggcc ggtaccagga cgagctcggc aacgacttct ccacgcgcat ccagggcacc
      121 cgcatcaagc accacatggg ctgggcggcg atcaagctct acgacaagtt cgggctcatc
      181 gcccgcgtcg agtgcacggc gaacgatgtg agttttttcc agcaccaccg cacggtggaa
      241 caccgggacg ggaccaagga gttcaagctg gcctccgtcc gcaagtcgat ctacagcctg
      301 ccggtcctcc gcgagctcct cggcgcagcg accttccgct acctcgactt tctcgccgcc
      361 ctcgaggacc cgcgccccgg cctccgggcg ctcgagaaga ttgccacccc cgtgcacgcg
      421 ggggagcgga gctaccgcgg cttcaacctg ttccacggcc ccgacctcga tctcttccgc
      481 acgatcctgc ggggcgagtt caccatcagc gggttccagg cccgccagct ccgccgccac
      541 atggccgact gctccgcggg ccagctctcg cgcggcctca agcggctgcg cctgcacggg
      601 ctcatcaaga agatcgggaa gcgctacaag tattacctca caacgctcgg gcggactgtc
      661 gccacggccg cgctcaagct gcgcgagctg gtgatcatcc cgatgctcaa tcaacccgct
      721 ccggcataac atggaagtct tgaccatttt ccacaggatc caactggtaa gagactaaat
      781 agatgccgag gccgaattca tcgcggcaca ctgtataggg gccgaacgta tacgtcaagc
      841 acctttgctg gcgggagctt ggcaactctc cccgccgatt gagcgaggcc tttgtccttg
      901 actccggacg ggaagcagag gatacttttt tgcctacgtc gctgaatctc taggctcgtc
      961 gtcacttacg gccatgctgg agcgcgggtg tcatgcctca tggcccagac ggcttaacgc
     1021 gtcatcggcc cacaaccgag cgtgccgacg ccccacccgc ccgtaacatc ccatcgatgc
     1081 cgggagatta ttttcatgcc cccgcgcaat caccccggtc ttgcgatcat tccagggaaa
     1141 ccacccgccc acagcgcgga ggagtgtgcg atccctttgc ctgcgctgtg ccgatgaacc
     1201 acttggatag gtgaggggcc tcggccgggg ccgggattcg tcggtaagga atgccgccat
     1261 gagatcgagg gctccgcgtc cccctggaaa acgtaagccg gcgcgcgtcg gtccgaaggc
     1321 ccccgcggac gccccttttc ccatcgtcgg cattggcgcc tccgccgggg gactggaggc
     1381 tctggagcag tttctgcgcc acgtcccggc tggcagcggc atggccttcg tcatcgtcca
     1441 gcatctcgac ccgacccaca agggcatcat gcccgaactg ctccagcgtg cgacggccat
     1501 gccggtcaac caggtcaagg atcgcacccg ggctcagccg gactgcgtct atgtgatccc
     1561 ccccaacaag gacctgtcca tcctgcgcgg ggtgctgcac ctgctggcgc cgacagcctc
     1621 gcgcggcctc cgcctcccga tcgatttctt cttccgctcg ctggccgcag accagcagga
     1681 ccacgccgtc ggcatcatcc tgtcgggcat gggcaccgac ggcaccctgg gtctccgggc
     1741 catcaaggaa aaagcgggtc tcgtcctggt gcaggccccg gcatcagcca agttcgacag
     1801 catgcccagg agcgccatag acgccggact ggccgacttc gtgggcccgg tggaggagct
     1861 gccaggaaag ctcctggcct ccgttcggca cgcccctctc gtgcccgcgc ccgggatccc
     1921 gctggaggcc agaacccaga gcgccctgga aaagatcgta ctcctgctcc gcgcacgcac
     1981 cggccaggac ttctcccact acaaaaagag caccgtgtac cgccggatcg agcggcgcat
     2041 gggtctgcat caggccgaca agatcgggac ttacgtccag tatctgcagg agaaccccca
     2101 ggagctggag ctgctcttca aggagctgct gatcggggtc acgagcttct tccgtgaccc
     2161 ggcggcctgg gagcaactga aagcgcatgg cattccggcg atcctggccc gccgccgcgc
     2221 cggccaagcg cttcgggcgt gggtccctgg ctgctccact ggcgaagagg cctactccct
     2281 ggccatggtc ttcaaagagg tgctggcgca gcgcaagacc acggatgcct attcgctaca
     2341 gatctttgcc accgatctgg accgagatgc catcgaccgc gcccgccaga gcgtctttcc
     2401 ggacaacatc gccgccgatg tgtcaccgga gcgtctgaag cgattcttca tcaaggacga
     2461 tcgcggtttc cggatccgca gagagatccg ggagatggtc accttcgccc agcagaacgt
     2521 cctcatggac ccgcccttta ccaagctgga catcctcagt tgccggaatc tgctgatcta
     2581 cctggacccg gaactgcaaa ggaaactcat tccgctgttc tactacagcc tgaaccccgg
     2641 cgggatcctg ttcctgggga acgcggagag tgtcgggcac gcttccggcc tgttcgcccc
     2701 gctgggcgtc aaggcacgcc tctaccgacg gaacgagtcc accctgctgc cggagccggt
     2761 cgaattgccc gccgcgttcg tccggggggc cccggaggca tcggaggtgc accgggcgcc
     2821 gattcccccc ggcagcctcc aggggctggc ggatcagctc atcctgcagc ggtatgcacc
     2881 ggcgacggtc ctcgtcaatg atcaggggga catcctctac atcagcggcc gaaccggcaa
     2941 gtacctggag ccggcggccg gcagggtcaa ctggaacgtg ctggccatgg cgcgtgaagg
     3001 gctgcgcgtc gagctggccg ccaccctgcg gaaggcgctc cggcagaagg agccggtcac
     3061 gcttcgccac gtgaaggtgc ggacgaacgg gggggagcag atcgtaaatg tcaccgtcca
     3121 gccgttggag gagccggagg cattgcgggg gatggggata gtcgtctttt cggacgtgcc
     3181 cgccgtcccc accccgaagg cgccgggccg ggcctcgcga tcctcccgtc gcagcgttac
     3241 gttggctact gtggagcggg agctcgagcg ggcccgcgac gaggcgcgaa tcatccggga
     3301 ggagatgcag acctcgcagg aagagctgaa gtccaccaac gaggagctgc agtccaccaa
     3361 cgaggagctg accacatcca aggaggagat gcagtccctg aacgaggaat tgcagacggt
     3421 caacgccgag ctgcagacca aggtggacga gctgtcgcag tcgaacaacg acatgttgaa
     3481 cctgctcaac agcacggata tcgccacggt gttcctggac ggcgcgcttc acatccggcg
     3541 gttcaccgcc cgggcgacct cgctcatcaa gctgatcccc ggggatgtgg ggcggcccgt
     3601 gaccgacctg gcgtgcgact tgctctacca gggactcccc gaggacgcgc gggaggtgct
     3661 ccggacattg agagtctgcg aaaagtagat cgccatcgag gatgggcgct ggttcctggt
     3721 gcgcatcctg ccctaccgaa cctccgagga caagatcgac ggcgtggtgc tcaccttcac
     3781 ggagatcacg atcgccaaag cgcaggaggc ggagctgcgc aaggcgctgg ccgaacgcca
     3841 gcgggatcac gctgatgggt aagagcagga ataagccgac gcaggccgcc gacctgcgcc
     3901 gccgcgcgga gaaggggctg aaagcccgcg agccggacgc cgatctgcag cgcagcgccg
     3961 ccgaaacgca gcggctcctc cacgagctac aggtccacca gatggagctg aagcttcaga
     4021 atgaagagct cctgcaggtg agggatgcgt tggaattgcg ggtcaaggag aagacggcag
     4081 aggtggtccg ccggaccgcg gcggagcaag aggtgcgcga gcaatcggag cagctccgct
     4141 cgctggccgc cgagttgacg ctggccgagc agagggaacg acagcggctg gcgtcggtgc
     4201 tgcatgatga acaccagcag ctcctggtgg gggcgaagct cc
//
LOCUS       DBNGZC010000976         3072 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1154264, whole genome shotgun sequence.
ACCESSION   DBNGZC010000976 DBNGZC010000000
VERSION     DBNGZC010000976.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1154264"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="AC16G0_16860"
     CDS             <1..212
                     /locus_tag="AC16G0_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HIR5101327.1"
                     /translation="VDSIELELHALAKSEIDSQKIGQMVMEHLRTLDDVAYVRFASVY
                     RQFKDVHQFVEEVKGILGSGKRTRE"
     gene            433..1125
                     /locus_tag="AC16G0_16865"
     CDS             433..1125
                     /locus_tag="AC16G0_16865"
                     /EC_number="1.3.1.76"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - protoporphyrin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 dehydrogenase/sirohydrochlorin
                     ferrochelatase family protein"
                     /protein_id="HIR5101328.1"
                     /translation="MRKYYPMMVDLTGKRCLVVGGGSVAERKVALLLECGATVEVVSP
                     ATTARLAALAEGGRIILRRRPIRTADMTGAFLVVAATDDPRVNGRVAERARLVGGLVN
                     VVDDPEACSFLVPSVVRRGDLTIAISTGGGSPALAKKLRQRLEQTIGPEYEEFLAALR
                     ELRLRAQGSIADPKERQAIYRRAIESDLFEHVARGDATAVAARIEDLLHAVPPAATGA
                     GTAEATVRSHDR"
     gene            1115..1933
                     /locus_tag="AC16G0_16870"
     CDS             1115..1933
                     /locus_tag="AC16G0_16870"
                     /inference="COORDINATES: protein motif:HMM:NF013725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C assembly family protein"
                     /protein_id="HIR5101329.1"
                     /translation="MTADLFIFYLALVGYLIGTLHYLLHIISKRQIIGTVATAATLAG
                     FAAHTLSQIVHVYTAGRPSFAGPYETLSFFAWATVLIYLIIEFRYRNRIMGAFALPIV
                     LLACSAAAALPRRIAELAPAAFHGVGLWVHVTFAIFGNAAFALTFCTGLMYILQERQL
                     KSRQPGTMHFRLPPLELLDDVGVKSLLFGFPLLTLALITGSIGAEQARGSFLSLRVRE
                     TWSIASWLIFAGLLYARLSVGWRGRKAAILAIIGFCLVLSTLIGVSLFRGRVIL"
     gene            2010..>3072
                     /gene="hemA"
                     /locus_tag="AC16G0_16875"
     CDS             2010..>3072
                     /gene="hemA"
                     /locus_tag="AC16G0_16875"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HIR5101330.1"
                     /translation="MEILVLGLSHKSAPIQIRERLFVPERDLPKALEMLGEPPELAER
                     MLVTTCNRVEVYAVTEGQVERAAAAITDCLARYHNLEQASFADKLYTYTAGEAVRHVF
                     RVASSLDSMVVGEPQILGQVKDAYAAARSLEAAGVILTNLLEQAFHVAKRVRTETGIA
                     SSAVSISSAAVELARKIFGSLAGRTVLILGAGEMAELALRHLLDDGVRSVLVANRTHD
                     RAVALAEQFHGRAVTYESFPQEMVAADIVISSTAAPHVILRKEDLQGIILQRRHRPIF
                     LIDIANPRDIDAACNDIDNVYLYNIDDLQAVVAANLKERQREAVRAEAIIEREVEVYL
                     TWLRQQDVVPTIVSLRSRVE"
BASE COUNT          480 a         1013 c         1023 g          556 t
ORIGIN      
        1 tcgtcgactc gatcgagttg gaactacatg ccctggcgaa atcggagatc gactcgcaaa
       61 agatcgggca gatggtcatg gagcatttgc gcacgctgga cgacgtggcc tacgtccggt
      121 ttgcctccgt gtaccgccag ttcaaggacg tccaccagtt cgtggaagag gttaaaggca
      181 tcctcggttc ggggaagcgg acccgggagt agcaggctgc tgaaaaaggc ccatctgcgg
      241 cgttggccga cctcggcgct cactgggacg tacctccagt acgtctcgct cggcctccgg
      301 ccggccgcct tgcatctgga cctttttgag cagcctgctg ggccgcgcgg ccagatggac
      361 tgccggggtt ggatgggtcc tcgccgtact ccctgcactg aacctcgatg gacggccgac
      421 acacctcacg gcatgcgcaa atactatccc atgatggtgg acctcaccgg gaagcgctgt
      481 ctcgtcgtgg gcggcggatc ggtggcggag cgcaaggtcg ccctcctcct ggagtgcggg
      541 gcgacggttg aggtcgtgag tcccgccacc accgccaggc ttgcggccct ggccgagggg
      601 ggacggataa ttctgcggcg gcgccccatc cggactgcgg acatgacggg ggcctttttg
      661 gtggtggcgg ccacggatga tccccgggtg aatggccgcg tggccgagcg tgccaggctc
      721 gtcgggggtc tggtgaatgt ggtggacgac cccgaggcgt gcagcttcct cgtcccctcg
      781 gtggtccgac ggggagacct gaccatcgcc atctccaccg gtggcggcag tccggccctg
      841 gccaagaagc tccgccagcg actggagcaa accatcggtc cggagtacga ggagttcctc
      901 gccgccctgc gcgagctacg cctgcgggcg cagggatcta ttgccgatcc aaaggagcgg
      961 caggcgatct accggcgggc catcgaatcc gacttgttcg aacacgttgc gcggggggac
     1021 gcgaccgcag tcgcggcccg gatcgaggac ctcttgcacg ccgtcccgcc ggccgccacg
     1081 ggcgccggga cggcggaagc taccgtgcga agccatgacc gctgacctct tcatcttcta
     1141 tctcgcgctt gtcggctacc tgatcggcac cctgcactac ctcttgcaca tcatcagcaa
     1201 gcggcagatc atcggcacgg tggccacggc cgcgaccttg gccgggttcg ccgcccacac
     1261 cctctcccag atcgtccacg tctatactgc cggccggccc tccttcgcgg gtccgtacga
     1321 gaccctttcc ttttttgcct gggccacggt cctgatctac ctgatcatcg agttccggta
     1381 tcggaaccgg atcatgggcg ccttcgcgct ccccatcgtg ttgctggcct gctcggccgc
     1441 cgccgcgctt ccccgtcgaa ttgccgagct ggcgccggcg gcattccacg gggtgggcct
     1501 gtgggtccac gtgaccttcg ccatcttcgg caacgccgcc ttcgcgctca cgttctgcac
     1561 cggcctgatg tacatcctcc aggagcggca actgaagtcg cgccagccgg ggacgatgca
     1621 ttttcgcctg ccgcccctgg aactgctgga tgacgtgggg gtgaagtccc tcctcttcgg
     1681 atttccgttg ctcacgctgg ccctcatcac cggatccatc ggggcagagc aagcccgggg
     1741 ctcgtttctc agcctgcgcg tgcgcgagac ctggtcgatc gcgagctggc tgatcttcgc
     1801 ggggttgctc tacgccaggc tgtccgtggg ctggcgaggc cgcaaggcgg ccatcctggc
     1861 gatcatcgga ttctgtctgg tcctctcaac gttgatcgga gtcagtctgt tccgggggag
     1921 ggtgatcctc taggggattg ggtcgggcgg agccgccggc agcgcacgcc gcggattcac
     1981 accggctgca gtctcccgct gattgagcca tggaaatctt ggtcctggga ttgagtcaca
     2041 agagcgcgcc gatccagatc cgggagcggc tattcgtccc cgagcgcgat ctccccaagg
     2101 cgctggagat gctgggggag cctccggagc ttgccgagcg catgttggtc accacgtgca
     2161 accgggtcga ggtgtatgcg gttaccgagg ggcaggtcga acgggcggcg gccgcgatca
     2221 ccgactgcct ggcgcggtat cacaacctgg agcaggccag cttcgctgac aagctgtaca
     2281 cgtacacggc cggcgaggcg gtgcggcacg tgtttcgggt cgcctccagc ctggactcca
     2341 tggtggtggg cgagccgcag atcctcggcc aggtcaagga tgcctacgct gcggcgcgca
     2401 gcctggaagc ggccggcgtc atcctgacca atctcctgga gcaggcgttc catgtggcca
     2461 agcgggttcg gaccgagacc ggcattgcca gctcggcggt ttccatctcc tcggcggctg
     2521 tggagctggc acggaagatc ttcgggagtc tggccggcag gaccgtcctc atcctcgggg
     2581 cgggcgagat ggccgagctg gccttgcgcc acctgctgga cgacggcgtg cgctcggtct
     2641 tggtggccaa ccggacgcac gatcgcgccg tggccctggc ggagcagttc catggccgcg
     2701 ccgtgaccta cgagagcttt ccgcaagaga tggtcgccgc cgacatcgtg atcagctcca
     2761 ccgccgcgcc ccatgtcatc ctgaggaagg aggacctgca gggcatcatc ctccagcgac
     2821 gccaccggcc gatcttcctt atcgacatcg ccaacccgcg cgacatcgac gcagcctgca
     2881 acgacatcga caacgtctac ctctacaaca tcgacgactt gcaggcggtg gtggcggcaa
     2941 acctcaagga gcggcagcgg gaggcggtgc gggccgaggc catcatcgag cgagaggtcg
     3001 aggtctacct cacctggctg cgccagcagg acgtggtccc gaccatcgtc tccctccgga
     3061 gtcgtgtgga gc
//
LOCUS       DBNGZC010000977         2807 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1744479, whole genome shotgun sequence.
ACCESSION   DBNGZC010000977 DBNGZC010000000
VERSION     DBNGZC010000977.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1744479"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..2578
                     /locus_tag="AC16G0_16880"
     CDS             113..2578
                     /locus_tag="AC16G0_16880"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140358 - P-type transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR5101331.1"
                     /translation="MTREQRTPSSSAETPHPEGVVDPVCGMRILSQDAVGSVVYLGKT
                     YHFCSTRCMDSFKANPKQYAQPVGDRSAAPSPADDREEVVYTCPMHPEVRQVGPGICP
                     KCGMALEPVTVAPPPTQIEYTCPMHPEIVRSEPGSCPICGMALEPRMISREDQGNPEL
                     TDMSRRFWIGLVLTLPVVVLEMGGHLTNLHMIIPPQVASWLQLAFATPVVLWAGWPFF
                     VRGWASVVRRSLNMFTLIAMGTGVAWGYSVVATLAPTLFPAAFRGEGGAVAVYFEAAA
                     VITVLVLLGQVLELRARERTSGAIRALLNLAPKTALRVSETGEDQEVALEAVVVGDRL
                     RVRPGDKVPVDGELVQGKSSLDESLVTGESMPVAKVPGDKVIGGTVNQTGSFVMRAER
                     VGRDTMLAQIVQMVADAQRSRAPIQRLADVVAGWFVPAVIAVAALAFIAWSIWGPEPA
                     LSYAMVAAVTVLIIACPCALGLATPMSIMVGVGRGAGAGVLIKNAEALERFEKVDTLV
                     IDKTGTLTEGKPAVTAVMPVEGLTADQLLGLAASVERGSEHPLAGAILAAAQAKQLSL
                     ADVQDFDAPTGKGVMGSVSGKRVALGNMKLIGDLGIDAGPLTQRAEALRAEGATVILA
                     AVDGRLAGLLAIADPIKGSTPAAIRGLKEDGIRIVMLTGDNRTTAQAVAAKLGIEEVE
                     AEVLPEDKGKVVKRLRDEGRVVAMAGDGVNDAPALAAADVGIAMGTGTDVAMESAGVT
                     LLKGDLEGIVRARRLSAATMRNIRQNLFFAFVYNTAGVPVAAGVLYPFFGILVSPVFA
                     AAAMALSSVSVIGNALRLRRVSL"
BASE COUNT          478 a          858 c          994 g          477 t
ORIGIN      
        1 cgcattgagg ggctgcggcc aagtgagggc ccggccggga tccagagctc gtggcgaatc
       61 ctcgagcctc ggacaccgag aggctcgtca cgggtaagag gaggcagggg agatgacacg
      121 agaacagcga accccatcat cctcggcaga gaccccccac ccagagggag tggtggatcc
      181 ggtctgtggg atgaggattt tgagccagga cgcggtgggc agcgttgtct acctgggtaa
      241 gacgtatcac ttctgtagta cacggtgcat ggacagcttc aaggccaatc caaagcaata
      301 tgcgcagccc gtcggggacc gctcggcggc gccttctcct gccgatgatc gagaggaggt
      361 ggtgtacacc tgcccgatgc accctgaagt gcgccaagtt ggccccggca tctgccccaa
      421 gtgcgggatg gcgctcgagc cggtcaccgt cgcccctccg cctacccaga tcgaatacac
      481 gtgcccaatg catccggaga tcgtgcggag cgagccggga tcgtgcccca tctgcgggat
      541 ggccctggaa ccacggatga ttagccggga ggatcaaggg aatccggagc taacggacat
      601 gagccgccga ttctggatcg gcctcgtcct cacccttccc gtggtggtcc tcgagatggg
      661 tgggcacctc acgaatctcc acatgatcat cccgccccag gtggccagtt ggctgcagct
      721 tgccttcgcc acgccggtgg tcctgtgggc cgggtggccg tttttcgtcc gcggctgggc
      781 ctccgtcgtc cggcggagcc tgaacatgtt cacgctcatt gccatgggca ccggcgtggc
      841 ctggggctac agtgtcgtgg ccactctcgc gcctaccctc ttcccggccg ctttccgggg
      901 cgagggcggc gccgtggcgg tttatttcga ggcagcagcg gtgatcaccg tgctggtcct
      961 gctcggccag gtgttggaac tgcgcgcccg tgagcgcacg agcggcgcca ttcgcgcact
     1021 gctcaacctg gcacccaaga cggccctgcg cgtgagcgag acaggtgagg accaggaggt
     1081 cgcgctcgag gcggtcgtcg tgggcgatcg cctgcgcgtg aggccgggcg acaaggtgcc
     1141 ggtagacggc gaactcgtcc agggcaagag tagcctggac gagtccttgg tgacgggcga
     1201 atccatgccg gtcgccaaag tgccgggaga caaggtaatc ggcggcactg tcaatcagac
     1261 cggcagcttc gtgatgcgcg ccgagagggt cggtcgcgat accatgctgg ctcagatcgt
     1321 ccagatggtg gcggacgccc agcgaagccg ggcgcccatc cagcgactgg cggacgtggt
     1381 ggccggctgg ttcgtgcccg cggtcatcgc cgtcgccgcg ctggcgttca tcgcctggtc
     1441 gatctggggt cccgagccgg ccctgagtta cgccatggtt gccgcggtga ccgtgctcat
     1501 catcgcctgt ccctgcgccc tgggtctggc aaccccgatg tcgatcatgg taggggtcgg
     1561 ccgcggcgcc ggggctggcg ttctcatcaa gaacgccgag gccctggagc gcttcgagaa
     1621 agtcgatacc cttgtcatcg ataagacggg gacgcttacc gagggcaaac ctgccgtgac
     1681 ggccgtgatg ccagtcgagg gcctcacggc ggaccagctc ctgggcctgg cggcaagcgt
     1741 ggaacggggc agcgagcatc ccctggcggg ggccattctg gccgcggcgc aggccaagca
     1801 actctcactg gcggatgtgc aggacttcga tgcgccgacg ggcaaggggg tgatgggcag
     1861 cgtgtctggg aagcgcgtgg cgctcgggaa catgaagttg attggagacc tcgggatcga
     1921 cgcggggcct ttgacgcaac gcgcggaggc gctccgggcc gagggggcca cagtcatcct
     1981 ggccgcagtg gacggccgcc tcgcagggct gctcgcaatt gccgacccga tcaaggggag
     2041 cacgccggcc gcgatccggg ggctgaagga ggacgggata cgcatcgtca tgctgacggg
     2101 cgataaccgg acgaccgccc aggccgttgc agccaagctc ggcatcgaag aagtggaggc
     2161 agaggtcttg cccgaggaca aggggaaagt ggtgaagcga ctgcgcgacg agggccgtgt
     2221 cgtggccatg gcgggggacg gcgtgaacga cgcgccggcg cttgccgccg ccgacgtggg
     2281 tatcgccatg ggcaccggca cggacgtggc gatggagagc gcgggcgtga ccctgctcaa
     2341 gggcgatctg gagggcatcg tgcgcgcccg gcggctgtcg gcagccacca tgcgcaacat
     2401 tcgccagaac ctgttcttcg ctttcgtcta taacacggca ggggtacctg tggccgcagg
     2461 agtgctctac cccttcttcg gaatcttggt gagcccggtt ttcgccgcgg cggccatggc
     2521 cctgagttcg gtctccgtca ttggaaacgc tctacggctc cgcagggtga gtctgtagaa
     2581 gtctgagccg atggcaggat ggcgttgccc ggccgccgaa gcggacgggg aagaacatgt
     2641 gctatactcc ccacaatctc gattcagccc ttatcgcgtt cgggagcttg gtgcatctct
     2701 gacactcgga acacgaaagc tgccttcagc ggatttgctg gcacagatgc cgaccgacac
     2761 atatagattt cagacgaccg tggagtggag tttcgcggtc ctgcgcg
//
LOCUS       DBNGZC010000978         3123 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2525735, whole genome shotgun sequence.
ACCESSION   DBNGZC010000978 DBNGZC010000000
VERSION     DBNGZC010000978.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2525735"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            946..2166
                     /gene="ltrA"
                     /locus_tag="AC16G0_16885"
     CDS             946..2166
                     /gene="ltrA"
                     /locus_tag="AC16G0_16885"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HIR5101332.1"
                     /translation="MLATLEQGVEGGKWFRLFDKVFSERNLLAAFQQVASKDGAAGVD
                     HVSVTQFERGLPETFWEVSDQLRNGTYRPQSIRRVHIPKPGTNETRPLGIPTVRDRVV
                     QAAVVNVIEPIFERDFAEHSYGFRPGRGCKDALRRVNELLKAGHVHAVDADLKGYFDS
                     IPHDRLMMRLRGKIADGPVLSLIESFLEANILDGLEEWNPTAGTPQGAVLSPLLSNIY
                     LDPLDHLMARAGFAMVRYADDFVILCRTPEEANRALELVRTWASENGLMLHPTKSKVV
                     DARTEGFDFLGYHFEEGKRWPREKSLTKFKDTIRAKTRRTHGDSLPKIIASLNRMLRG
                     WFEYFKHSRGWIFPELDGFIRRRLRGILQKRAKRRGTGHGLANQRWPNAFFAAHGLFS
                     LEQAHAEARQPSRR"
     gene            complement(2117..2446)
                     /locus_tag="AC16G0_16890"
     CDS             complement(2117..2446)
                     /locus_tag="AC16G0_16890"
                     /inference="COORDINATES: protein motif:HMM:NF024172.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101333.1"
                     /translation="MVGRESGEIRLDVIESASIVELDDVIDNACLEGTTVNTDEWNGY
                     NRIGKRHGRVHRTIDHYVMIGVGRTDWIRSLPPPNRTGGSPAYGSPVDGLTSERVDGP
                     RRGLVPD"
     gene            complement(2328..2972)
                     /locus_tag="AC16G0_16895"
     CDS             complement(2328..2972)
                     /locus_tag="AC16G0_16895"
                     /inference="COORDINATES: protein motif:HMM:NF024170.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR5101334.1"
                     /translation="MDFSLLDYLDEDACYARLVALFHPDGLACPRCGERQRLGIHRCH
                     RAPLVDFQCGGCGRVFNAFTGTSLQGTHRRPAQLLLILRGVAQGTPTAQMARELGCDR
                     KELLALRHRLQEHARLHLDRNPLDEAAVEADEMYQNAGEKRGRARRPRGSAAPPRQQP
                     EGPWHLRHRSTAGGRRGRSRVGGDPVGRDRVGEHRRVGRCDRQRVPGGDDGEHR"
BASE COUNT          588 a         1003 c         1043 g          489 t
ORIGIN      
        1 cacgcccggc actcctccca ccccagccgt tgcttcaggt cccggaaccc gtcctcctgc
       61 cggaaccggg cggcgaacaa ctccaccacc tgcaacccgc tcaactccac cgccgaaata
      121 atcaaccacg cccagaggac gtggtattag gaaggccccc ccgagggggc cgttcacgtg
      181 gggaagcccc cgttgggggc gagccgatca gcccacccag gctcagggcg gggcggccga
      241 actgacccgg tcccgcaggc gtttccccgg cgttcgtccc agtggccgga tcgacgacgt
      301 cacctgaggg ccctgggttc atccgatcgg atctccgacc gaggggcaga gcccaaaggc
      361 acgccggatc gctcgaagct cttcgggtca cacggccaga ggccgtggct tgtgcgtctg
      421 taagtgtgca aggtcaagga ggtgcaagtc ctcctcaggt agacgctctc cggcctgtaa
      481 ccgacggaaa ccgcgtcgcc gcgaggcggg gtgggaagca acccgaggtg aacagccagt
      541 ccgcaccgaa agtgaactcg attcggcctt ctcgcgtggc gagcctgcaa gccaaaggcg
      601 aagcccaaac cggcgcgagc cggatgcctc ccacgctgag gggtagcgtg tcaaagcgat
      661 gggagggccg aggtggtggt tggaggcgga atggctggac ggccctgcac aggacacaga
      721 gagacctgcg tggtccagac gggcgcgagc ccgccatgca ggagtcagag gagccatagt
      781 agcgaggaag ccgggtaacg ccgggggagc gaagggctcc aggaagatgg atgcgacatg
      841 acccgaccga ccgaagacaa accggcgata gtgccgctgg ctcaacaagc cggagagatc
      901 ctctcgcgat ggcgatgggt cgaaccgtcg gcctggaccg tgcgcatgtt ggcgactctc
      961 gaacagggag tggagggagg caagtggttc cggctgtttg acaaggtctt ctcggagcgg
     1021 aacctgctgg cggcgttcca gcaagtggcg tcgaaagacg gcgcggccgg agttgaccac
     1081 gtctccgtga cgcaattcga gcgagggctg ccggagacgt tctgggaggt ctcggatcaa
     1141 ttgcgaaacg gcacgtatcg gccccagtcg atccgacgcg tccacattcc caagccggga
     1201 accaacgaga cacggcccct ggggatcccc acggtccgcg atcgggtggt ccaggcggcg
     1261 gtggtgaacg tgatcgagcc gattttcgag cgcgacttcg ctgagcacag ctacgggttc
     1321 cgcccgggac gcggctgtaa agacgctttg cgccgggtta atgagctgtt gaaggcggga
     1381 catgttcacg cggtggacgc cgacctgaaa ggctacttcg actcgatccc tcacgaccgc
     1441 ctgatgatgc gcctgagagg gaagatcgcc gacggcccgg tgctgtcgct gatcgaatca
     1501 ttcctggagg cgaacatcct ggacggcctg gaggaatgga accccacggc aggcacgccc
     1561 cagggggcgg tcctgagccc gctgttgagc aatatctatc tcgacccgct cgaccatctg
     1621 atggcgcgag caggcttcgc gatggtgcgc tatgccgacg acttcgtgat cctatgccgg
     1681 acgcccgaag aggcgaaccg cgctctggaa ctggtacgga cgtgggcgtc ggagaacggc
     1741 ctcatgctgc acccgacgaa gtcgaaggtg gtcgacgcga ggaccgaggg cttcgacttc
     1801 ttgggctacc acttcgagga aggcaaacgc tggcctcggg agaagagcct gacgaagttc
     1861 aaggacacga tccgcgccaa gacccgacgg actcacggcg actcgctgcc caagatcatc
     1921 gcgtcgttga atcgcatgct tcgcggctgg tttgagtact tcaaacacag cagaggatgg
     1981 atcttcccgg aattggacgg cttcatccgc cgccggcttc ggggcatcct ccagaagcga
     2041 gcgaagcggc gcgggaccgg gcatggcctc gccaatcaac gctggcccaa cgccttcttc
     2101 gccgcgcatg ggctcttcag tctggaacaa gcccacgccg aggcccgtca accctctcgg
     2161 aggtgagacc atcaaccgga gagccgtatg cgggagatcc gcccgtacgg ttcggagggg
     2221 ggagggaccg aatccaatcg gtccttccta cccctatcat tacatagtga tcgatcgtcc
     2281 gatggacccg gccatgccgc ttgccgatgc ggttgtagcc gttccattca tcggtgttca
     2341 ccgtcgtccc ctccaggcac gcgttgtcga tcacatcgtc caactcgacg atgctcgccg
     2401 actcgatcac gtccaaccgg atctcccccg actcgcgacc gaccacgccg gccaccggcg
     2461 gtcgatcggt ggcgaaggtg ccatggcccc tccggctgtt ggcgcggcgg cgcggcggat
     2521 cctcggggtc gtcgtgcacg accccttttt tcccccgcgt tctggtacat ctcgtcggcc
     2581 tcgaccgcgg cctcgtccag ggggttgcgg tcgaggtgga gccgggcgtg ctcctggagc
     2641 cgatgcctca gggccagcag ctccttgcga tcgcagccca actcgcgggc catctgcgcc
     2701 gtgggggtgc cctgagcgac gccgcgcagg atcagcagga gctgagcggg acggcgatgg
     2761 gtcccctgca gggaggtccc cgtgaaggcg ttgaagaccc ggccgcaacc gccgcactgg
     2821 aagtccacga ggggcgcgcg atggcagcgg tggatcccca ggcgctgtcg ctccccgcaa
     2881 cgggggcatg ccaggccgtc ggggtggaac agggcgacga gtcgggcgta gcaggcgtcc
     2941 tcatccaggt agtcgagcag ggagaagtcc atggtcagga cctccgtgca acacgtcgga
     3001 tcccccgttg aggacaccac cgctcggagc acccgtcaag aggcggagca ccggtctccc
     3061 ctcatgagcc atattacggc aatcaagagg ttcccaaaac ggcctgggcg acccctatgc
     3121 cct
//
LOCUS       DBNGZC010000979         1289 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_866220, whole genome shotgun sequence.
ACCESSION   DBNGZC010000979 DBNGZC010000000
VERSION     DBNGZC010000979.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_866220"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..679
                     /locus_tag="AC16G0_16900"
     CDS             <1..679
                     /locus_tag="AC16G0_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HIR5101335.1"
                     /translation="NAPEIGKAAVKVLEAAGFEVFLADKKCCGRPMISKGLIQAAKRN
                     AAYTVDRLSEFARQGIPVVGCEPSCILTFRDEYPDLLDDPRAEHLAKHTFMIEEFLVG
                     LHEKGELDLPFSDGARSILLHGHCHQKALIGSSPSLQVLRLLPGVKVEEVDSGCCGMA
                     GSFGYEKEHYELSLAIGSRRLFPAVKARGPECEIVAAGISCRQQIAHGTGRRARHLVE
                     VLAAALP"
     gene            718..>1289
                     /gene="msrA"
                     /locus_tag="AC16G0_16905"
     CDS             718..>1289
                     /gene="msrA"
                     /locus_tag="AC16G0_16905"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011318025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR5101336.1"
                     /translation="MAWFHSERKLRAPTPGEALPGRSQRMAVPAVHAVSGVRLEPPFP
                     SGTELALFGMGCFWGAERCFWVQPGVYVTAVGYAGGYTPNPTYEEVCSGLTGHAEVVR
                     VVFDPAVVTFDALLRIFWESHDPTQGMRQGNDIGTQYRSAIYCHGEAQLAVAGASRAA
                     YQQALTAAGLAPITTEIRDAPEFYYAEAYH"
BASE COUNT          207 a          441 c          422 g          219 t
ORIGIN      
        1 caacgcgccc gaaatcggga aggccgcggt caaggtcctg gaggcggcgg gtttcgaggt
       61 cttcctggcc gacaagaaat gctgcggccg gcccatgatc tccaagggcc tcatccaggc
      121 agccaagcgg aacgcggcat acaccgtgga ccggctctcg gaattcgcgc gccagggcat
      181 ccccgtcgtg ggctgcgagc cgagctgcat cctcacattc cgcgacgagt accccgatct
      241 gctcgacgat ccccgcgccg agcacctggc caagcacacc ttcatgatag aggagtttct
      301 ggttggactg cacgagaagg gggaacttga cctcccattc tcggatggcg ccagatccat
      361 cctcttgcac gggcactgcc accagaaagc cctgatcggg tcgagtccca gcctgcaggt
      421 ccttcgcctg ctccctggcg tcaaggtcga ggaggtggat tccggctgtt gtgggatggc
      481 cgggtccttc ggctacgaga aggagcatta cgagctgtcc ctggccatcg gcagccgccg
      541 gctcttcccc gcggtcaagg ccagggggcc ggagtgcgag atcgtggccg ccggcatttc
      601 gtgtcgccag cagatcgccc acggcaccgg ccggcgcgcc cggcacctgg tggaggtcct
      661 agctgccgca ctcccctagc gtcgctgtcc gatcccgcag ttgagcggag gcaaggtatg
      721 gcgtggtttc attcagagcg gaaactgagg gcgccgaccc caggggaggc cctgccgggt
      781 cgcagccagc gtatggctgt ccccgccgtg cacgccgtct ccggcgtccg gctggaaccc
      841 ccgttcccgt ccggaaccga gttggccctc ttcggcatgg gctgcttctg gggtgcggag
      901 cgttgcttct gggttcagcc aggcgtctac gtcacggcgg tcgggtatgc cggcgggtat
      961 acacccaacc ccacctacga ggaggtctgc agcggcctca ccggccatgc cgaggtcgtg
     1021 cgtgtggtct tcgacccggc ggtcgtcacc tttgacgcgc tccttcggat cttctgggaa
     1081 agccacgatc cgacccaggg catgcgccag ggaaacgaca tcggcaccca gtaccgatcg
     1141 gccatctatt gccacgggga agcgcagcta gctgttgctg gggcgtcgcg ggcggcctac
     1201 cagcaggccc tcaccgcggc cggcctcgcc cccatcacga ccgagatccg ggacgcgccg
     1261 gagttctact acgccgaggc ctatcacca
//
LOCUS       DBNGZC010000980         3019 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_767673, whole genome shotgun sequence.
ACCESSION   DBNGZC010000980 DBNGZC010000000
VERSION     DBNGZC010000980.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_767673"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="AC16G0_16910"
     CDS             <1..868
                     /locus_tag="AC16G0_16910"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR5101337.1"
                     /translation="PVTDPSSVAMERLSKLVKERTGGKVDFTHFGGGQIGGERDMVEG
                     VQLGTVGMVITGVTGHPIYDAFFLPYVFRDREHMWKVVYGPIGDEWNERMIKERGMRV
                     LGYMYRGPRQLTTTKRMVKTAGDVKGLKIRVPEIAPMLATWKALGASPTPMAWPEVFT
                     GLQQGSIEGQENPLELIASSKLWEVQKYLTLTNHVRVAWIVLLDERLWKRMTPETQQI
                     MTSSWREVSADMEKQILASEQKLIDEIKSHGVTVTEPDVESFRQAVKDVWRDVAPKAW
                     GPGVYERIQATP"
     gene            1350..1580
                     /locus_tag="AC16G0_16915"
     CDS             1350..1580
                     /locus_tag="AC16G0_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HIR5101338.1"
                     /translation="MRRTTVFAEESVLNALRAIARRRGITLADVTREALSAYVSKHRG
                     KGKPLSIIGIGRSGRRDVAERAEELLKEGFGR"
     gene            1580..1996
                     /locus_tag="AC16G0_16920"
     CDS             1580..1996
                     /locus_tag="AC16G0_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005193455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR5101339.1"
                     /translation="MPGLLLDSGALYALADRDDKWHARMVEALEGHPGDRVVPVTVLT
                     EACYLLNTHLGIAAERRLIRAILGGELLLEGVILSDLGRADQVLQKYSDANIGLVDAT
                     VVAIAERLGIRTVATTDRRHFSLFRPKHCRAFELLP"
     gene            2764..>3019
                     /locus_tag="AC16G0_16925"
     CDS             2764..>3019
                     /locus_tag="AC16G0_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5101340.1"
                     /translation="MGSRVQGKVVIVTGAGSIGPGMGNGKASSILYAREGARVFLVDQ
                     NLGAAQETERAIAAEGGAGLPYQCDVTSSAACQRMAEACLA"
BASE COUNT          552 a          944 c          973 g          550 t
ORIGIN      
        1 gccggtcacc gatcccagct cggtggccat ggagcggctc tccaagctgg tcaaggagcg
       61 gaccggcggc aaggtcgact tcacccactt cggcgggggg cagatcggcg gcgagcggga
      121 catggtcgag ggcgtccagc tcgggaccgt cggcatggtc atcaccggcg tgacggggca
      181 cccgatatac gacgccttct tcctgccgta tgttttccgg gatcgcgagc acatgtggaa
      241 ggtggtctat gggccgatcg gggatgagtg gaacgagcgc atgatcaagg agcggggcat
      301 gcgcgtcctc ggctacatgt accggggacc gcggcagctc accacgacca aacgcatggt
      361 caagacggcg ggagacgtga aaggattgaa gatccgcgtg cccgagatcg cgcccatgct
      421 cgccacctgg aaggcgctgg gagccagccc gacccccatg gcgtggcccg aggtcttcac
      481 cggcctgcag caggggtcca tcgagggaca ggagaacccc ctggagctca tcgcgtcgtc
      541 caagctgtgg gaggtgcaga agtacctgac gctgaccaat cacgtccgcg tggcctggat
      601 cgtcctcctg gacgagcgac tctggaagcg catgacgccc gagacgcagc agatcatgac
      661 cagctcgtgg cgcgaggtct cggccgacat ggagaagcag atcctggcca gcgagcagaa
      721 gctgatcgac gagatcaagt cccatggggt cacggtcacc gagccggacg tggagtcgtt
      781 ccggcaggcg gtgaaggacg tgtggcgcga tgtcgccccc aaagcctggg gaccgggtgt
      841 ctacgagcgc atccaagcca ccccctgaag aggcgcaccc acgatcggcg atcggccgcg
      901 tcatttcaat ggcgcgaccg atcgctccgc tcagggttac gcgctccggg ctcgataact
      961 ccagaggagt tgcatcgacc aatctcccgg actcccaggc agggcgagca ctggggcttg
     1021 gttaacgcct caaatgtcct tccgcgtgcg gcaagttttc cccgcatggc gcctctgcat
     1081 ctacatatcc cgctttagtc gatagagcga atcgcccggc gcgccgtcgg gagcctgacc
     1141 gttgcctttg cgccaggctc cccgaggaca tggaggccaa gaatcgggac cagcccaccg
     1201 taagaacagg aggttagtcc ggatatggtg tccggtaccc ttcacgccct gtgctcccga
     1261 ccgcctattc cagcgtcttc ttggctcaac agatctgttg acacgtaagc gcacgtatgt
     1321 gagcatacgt atgctagacg gaggagccga tgagacggac gacagtattc gccgaggagt
     1381 cagtgctgaa tgccttgcgt gcgatcgccc gacgccgagg tatcacgctg gctgacgtaa
     1441 ccagagaggc gctctccgcc tatgtctcca agcaccgcgg caagggcaaa ccattgagca
     1501 tcatcggcat cgggcggagc ggccggcggg acgttgcgga gcgcgccgag gaactcctca
     1561 aggaaggctt tggccgctga tgcctggact actgctggat agcggtgcgc tctatgccct
     1621 tgcggaccga gacgacaaat ggcatgcacg gatggtcgag gcgctagagg gccatccggg
     1681 tgatcgggtc gtcccggtca cggtgctgac cgaggcgtgc taccttctta atactcacct
     1741 ggggatcgcc gcggagcgca ggctgattcg agccatcctt gggggagagc tgctcctaga
     1801 gggagtgatc ctgtcggacc tggggcgggc tgaccaagtc cttcagaagt attcggacgc
     1861 gaacatcggc ctggttgacg cgaccgtggt ggccatcgcc gagcgcctcg gcatccgtac
     1921 tgtcgccacc accgaccgca gacacttcag tctcttccgc cccaagcact gtcgcgcctt
     1981 cgagcttctc ccgtagaacc agcacgttgc ccgctcgcct ccctgatttg ccagcatcct
     2041 tcgtcgcgcc ggcgccccgg gctgactcca tacccccgcg cttcggcgtc cacccggcgc
     2101 gttgtcggga gcctggccgt tgcctttgcg ccaggctccc cgacgacata gccggccaag
     2161 aattggatcc agcccctcgt agaatccaga ggttagcccc gatatggaac ctgctgtccg
     2221 cgatgtcaga gctagtcgga aagatgactc ttctcttctc ccttcggtaa agcaaggcgt
     2281 gcccaccgcc atgtccggcg gcatccggac aggcttatac tgcatgggga tagcacgccg
     2341 gtggtaagga ctccctattt ttcaattcct ttctcgtcgc cgtacctgcg tcgcgaaaca
     2401 cccccacgac cggcaaatcc cacgagccct ttcgcgtctc gtctcacctg cctcttgtgt
     2461 aaagacacta cgccttccat ctctcgatgt ctgtcaggga aagccgatta cttcggcgtg
     2521 ctcgtcaggg aaatccccaa gaatcgtgtc ggggaaaacc ctggggcgag cttatccctc
     2581 gaaggctagc tcttggtgct ggcgcttgac caactgccca ttacgagcgc gttttggctt
     2641 gacgcaaacg gtctgtctcg ggcatgctcc gcccgtctcc tggacgaccc cgcacgttgc
     2701 gccgggtttc gttgcgctgc cgcgtggagc gagactggtc cggaccacga gaggagttgt
     2761 gagatgggaa gtcgggtgca gggaaaggtt gtcatcgtga ccggggcggg atccatcggt
     2821 ccggggatgg gcaatggaaa ggcttccagc atcctgtatg cgcgcgaggg cgcacgggtt
     2881 ttcctggtgg accagaacct gggggcggcc caggagacgg agcgggcgat cgcggcggaa
     2941 gggggtgcgg ggcttcccta ccagtgcgat gtgacctctt ccgcagcctg ccagcgaatg
     3001 gcggaggcct gtctcgcgg
//
LOCUS       DBNGZC010000981         2113 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_182230, whole genome shotgun sequence.
ACCESSION   DBNGZC010000981 DBNGZC010000000
VERSION     DBNGZC010000981.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_182230"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="AC16G0_16930"
     CDS             complement(<1..626)
                     /locus_tag="AC16G0_16930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010511595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-5-phosphate-dependent protein subunit
                     beta"
                     /protein_id="HIR5101341.1"
                     /translation="MLDLRVNEEVLKSTVKFCREKGITLPTFAMMQDPRLMPESIRKI
                     LAATSLWDVHPANLYRITWKNEPKERGGLFGNVNHIVLPTTLTGCSANILVLLGRYFP
                     TGAHKVGATFGCLVPRLVTGQFNPLTTKAVWPSTGNFCRGGAYLSALLHCHAIAILPE
                     GMSRERFEWLRKIAGEVIATPGCESNVKEIFDKCWELRASGQDLMIFN"
     gene            complement(690..1871)
                     /locus_tag="AC16G0_16935"
     CDS             complement(690..1871)
                     /locus_tag="AC16G0_16935"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR5101342.1"
                     /translation="MKFRYICSECGLDFPIDPKLMVCPQCSLAQVADQPLRGVLEVEL
                     RGKVPASWEIPWLLPLEEQYFPPIPVGGTPLWAPQRLRRELDFPGLYIKDDGLNPTCS
                     LKDRASFLVSAFAAKFGIQEIVLASSGNAGSSMAGVGAAAGQKVTLFLPKTAPVAKLI
                     QALQYGAKVFRVGGSYDDAYDLSLAYTAKFGGLSRNTAYNPMTIEGKKTVSLEMFRQL
                     GRAPDYVFVSAGDGCILSGVYKGFRDLRSLGLLDRLPTIFATQAEGSDALARAFDSGV
                     FLRAAGHTVADSISVDVPRNGFHTLRQLREHGGQVVRVSDEQILAAQARLSRSAGLFA
                     EPAGAAAFAGFLEARPRLPKAAVTVVLVTGNGLKDTATAGRSVRIPEKIITGLDEVNG
                     G"
BASE COUNT          361 a          685 c          696 g          371 t
ORIGIN      
        1 tggttgaaga tcatgaggtc ctggcccgag gcccggagct cccagcactt gtcgaagatc
       61 tccttgacgt tggactcgca accgggggtg gcgatgacct ctcccgcgat cttccggagc
      121 cactcgaacc gctctcgcga catcccctcg ggcaggatgg cgatggcatg gcagtggagc
      181 aaggccgaga ggtaggcccc gccccggcag aagtttcccg tggagggcca gacggccttg
      241 gtggtcaggg ggttgaactg accggtgacc aggcgcggca cgagacaccc gaaggtagcc
      301 cccaccttgt gggcgccggt gggaaagtag cgtcccagga ggaccaggat gttggccgaa
      361 cagccggtga gcgtcgtggg cagcacgatg tggttgacat tcccgaagag tccgcctcgc
      421 tccttgggtt cgttcttcca cgtgatccgg tagaggttgg ccgggtggac atcccacaga
      481 ctggtcgcgg ccaggatctt ccggatggat tccggcatga gccgggggtc ctgcatcatg
      541 gcgaaggtgg gcagtgtgat gcccttctcc cgacagaact tgacggtgct cttcagcact
      601 tcttcattca cccgtaggtc caacaccgct gtctctcttc tcggttgagg atttgcaggg
      661 ggtcgccgcc ccgtggcgcg gctgcttgct cacccgccgt tcacctcatc caagccggtg
      721 atgatcttct ccggaatccg cacgcttcgg ccggccgtgg cggtatcttt gaggccgttg
      781 cccgtgacca ggacgaccgt cacggccgcc ttgggaagcc ggggccgcgc ctccaggaat
      841 ccggcgaagg ccgccgcccc ggccggctcc gcaaacaggc cggccgaacg cgacagtcgc
      901 gcctgggcgg ccaggatctg ctcgtcggac acccgcacca cctgtccgcc gtgctcccgg
      961 agctgccgga gcgtatggaa gccgtttctc ggcacgtcca cggaaatcga gtcggccaca
     1021 gtatggccgg ccgcgcgcag gaagaccccc gaatcgaagg cccgggccag cgcgtccgag
     1081 ccttccgctt gggtggcgaa gattgtgggc agtcgatcca ggaggcccaa ggaccgcagg
     1141 tcccggaagc ccttgtagac cccggagaga atgcagccgt cgccggcgga cacgaagacg
     1201 taatcgggcg cccggcctag ctgacggaac atctccaggg acacggtctt cttgccctcg
     1261 atggtcatgg ggttgtaagc ggtgttgcgg ctcaggccgc cgaacttggc agtgtaggcc
     1321 agggacaggt cgtaggcgtc gtcgtaggaa ccgcccaccc ggaatacctt ggcgccgtac
     1381 tgcaaggcct ggatgagctt agccacaggg gcggtcttgg gcaggaagag cgtcaccttc
     1441 tggcccgccg ccgcccccac gccggccatg gacgagccgg cgttgccgga ggaagccagg
     1501 acgatttcct ggatgccgaa cttggccgcg aaggccgata ccaggaacga ggcccggtcc
     1561 ttcagggagc aggtaggatt caggccgtcg tccttgatat acaggccggg aaaatccagc
     1621 tcacgccgga gccgttgcgg cgcccagagc ggcgtccctc ccacaggaat gggcggaaag
     1681 tactgctcct ccaagggcag cagccagggg atctcccagc ttgccgggac cttcccccgc
     1741 agttcaacct ccagcacccc gcgtagcggc tggtctgcca cctgggccag ggagcattgc
     1801 gggcagacca tgagcttggg gtcgatggga aagtcgaggc cgcattcgct gcagatgtag
     1861 cggaatttca tcagacaacc cgaattcttc ccgcggagcc gatgacgccc cctcgagcga
     1921 acagggcgag gcgctcccgc tggtatatgc catccccccg gaggcgcctc gcctgtggcg
     1981 attcgctact cggcggcctt caccgcctgc aggaacgcat tcatcatctc cgtaccctcg
     2041 gccccgaact tctggctgat gatcttggtg acctcgggtg tggtcttgtc cttgaatgcc
     2101 ttcttctgct cgt
//
LOCUS       DBNGZC010000982         2455 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_965703, whole genome shotgun sequence.
ACCESSION   DBNGZC010000982 DBNGZC010000000
VERSION     DBNGZC010000982.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_965703"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..99
                     /locus_tag="AC16G0_16940"
     CDS             <1..99
                     /locus_tag="AC16G0_16940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020148745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR5101343.1"
                     /translation="PQTGEAIKIPAKRVVKFRVAKAMKDSVLGGKK"
     gene            455..>2455
                     /gene="glgB"
                     /locus_tag="AC16G0_16945"
     CDS             455..>2455
                     /gene="glgB"
                     /locus_tag="AC16G0_16945"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HIR5101344.1"
                     /translation="MATLTKEVIRAIVRGEHGDPFGVLGPHAVKKGGRSVVAVRAFCP
                     GAEKVTVLPMGSDRPPRPMDRIHPEGLFEAVFPQSQAPTGYRLEVADSAGSRQVEDPY
                     RFPSTLSDFDLLLIGEGRHYRSYDKLGAHLLTLDGVPGVLFAVWAPNARRVSVVGDFN
                     RWDGRVHPMRLHPGNGIWEIFLPDLQAGMLYKFEILSRTSGLLALKADPYAFAFERIN
                     PPTASLVTDLEYQWTDAEWMAERAARNALAAPIAIYEVHLGSWRRNAQGEFLTYREAA
                     EALGQYVVEMGYTHVELLPITEHPFYGSWGYQTLGLYAPTGRYGSPQDFMAFVDHLHG
                     QGIGVILDWVPAHFPRDPHGLAYFDGTHLYEHDDPRQREHPDWGTRIFNYGRNEVANY
                     LIGSALFWLDRYHIDGLRLDAVASMLYRDYGRKEGDWIPNQYGGRENLEAVAFLKRFN
                     ETVYKYHPDVMTIAEESTAWPMVSRPTHLGGLGFGFKWNMGWMHDLLFYMSRDPIYRK
                     HHHNNLTFGLLYAWHENFILPLSHDEVVHGKGSLQQKMPGDDWQKFANLRLLFAYQWA
                     QPGTKLLFMGGEIGQWREWNHDGSVDWDFARHGNHQGVQNWVRDLNRLYTSEASLFDL
                     DFEPAGFDWIDCNDPDQSTLSFLRFSRDGAEAVAVVFNFTPVP"
BASE COUNT          450 a          786 c          804 g          415 t
ORIGIN      
        1 ccccagaccg gtgaagccat caagataccc gccaagcgcg ttgtcaaatt ccgggtggcc
       61 aaggccatga aggactccgt gctcggagga aagaagtagg ggcggtaccc tgcgaagtac
      121 gggccccggc ggaaaacccc aggacgacca agtcgggggt tgccgccggg gtccgatccc
      181 ttcagcccca ggcacgacac cggtgcgggc ggtgctcgct tggaaccggc tgcgcccgcc
      241 tccacggcag cccgacaaat cttcgatcaa tcaacctctg acgaagtctg ccaccagagc
      301 cgaatcttcc cgggagcggg ctgatctcgg gccaaggcct gtgaccccgg agactccgga
      361 tgattgtcaa tcaactctga tgtgatatgc tgggctcgcc gaggaccgcg gtcatataga
      421 atcattcggc aagagggcag ggaggcccgg aacagtggcg accctcacca aggaagtcat
      481 ccgagccatc gtccgagggg agcacgggga cccctttggg gtcctcggcc cgcatgccgt
      541 caagaagggc ggaaggagtg tcgtcgccgt ccgcgcgttc tgtccggggg cggagaaggt
      601 cacggtcttg ccaatgggat cggaccgacc gccgcgaccg atggaccgga ttcatccgga
      661 ggggctgttc gaagcggtct ttccgcagag tcaggccccg acaggctatc gactggaagt
      721 ggcggacagc gcgggtagcc gacaggtcga ggacccatac cgcttcccct ccacgctcag
      781 cgatttcgat ctgctcctga tcggtgaggg gagacattat cgatcttacg acaaactggg
      841 ggcgcatctt ttgaccctgg atggcgttcc gggtgtcctc ttcgccgtgt gggcgcccaa
      901 cgcgcgtcgg gtcagcgtgg tgggggattt caatcgctgg gacggccggg tgcaccccat
      961 gcggctgcat ccgggcaacg gtatctggga gatctttctc ccggacctgc aagcggggat
     1021 gctctacaag ttcgagatcc tgtcccggac cagcgggctc ctggcgttga aggcggaccc
     1081 ctacgccttc gccttcgagc ggatcaaccc accaaccgcc tcgctggtga ccgatctcga
     1141 gtaccagtgg accgacgcgg aatggatggc ggagcgtgcc gcgcggaacg ccctggccgc
     1201 ccccatcgcc atctacgagg tgcaccttgg gtcgtggcgg cggaacgcgc agggcgagtt
     1261 cctgacctat cgggaagcgg ccgaggcgct gggccagtac gtggtggaga tgggctacac
     1321 gcacgtggag ctcctgccga tcacggagca cccgttttac ggctcctggg gctatcagac
     1381 gctcggcctg tacgcgccga cgggtcgata cggcagcccg caggacttca tggccttcgt
     1441 ggaccacctg cacgggcagg ggatcggcgt catcctggac tgggtgcccg cgcacttccc
     1501 gagggatccc cacggcctgg cgtacttcga cgggacccac ctgtacgagc acgatgaccc
     1561 gcgccagcgc gagcacccgg attgggggac gcggatcttc aactacggac ggaatgaggt
     1621 ggccaactac ctcatcggga gcgcgctctt ctggctggac cggtatcaca tcgacggcct
     1681 gcgtcttgac gccgtcgcct ccatgctcta ccgggattac ggccgcaagg agggagactg
     1741 gatcccgaac cagtacggcg ggcgtgagaa cctggaggcg gtcgccttcc tgaagcgttt
     1801 caacgagaca gtgtacaagt accacccgga cgtgatgacc atcgcggagg agtccacggc
     1861 ctggcccatg gtttcccggc cgacgcacct gggcggcctg gggttcggct tcaagtggaa
     1921 catgggttgg atgcacgacc tgctcttcta catgagccgg gatcccatct accggaagca
     1981 ccaccacaac aatctcacct tcggcctgct gtacgcatgg cacgagaact tcatccttcc
     2041 cctgtcccac gacgaggtgg tccacgggaa gggctcgctc cagcagaaga tgccggggga
     2101 cgactggcag aagttcgcca acctgcgcct gctcttcgcc taccagtggg cccagccggg
     2161 cacgaagctg ctcttcatgg ggggagaaat cgggcagtgg cgcgagtgga accacgacgg
     2221 atcggtggac tgggacttcg cgcggcatgg caatcaccag ggcgtgcaga actgggtccg
     2281 cgacctgaac cggctctaca cgtccgaggc atcgctcttc gacctcgact tcgagcccgc
     2341 aggcttcgac tggatcgact gcaacgaccc ggaccagagc acgctgtcct tcctccggtt
     2401 ctcccgcgac ggagccgagg cggtggcggt cgtcttcaac ttcacgcccg tgcct
//
LOCUS       DBNGZC010000983         2973 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2628883, whole genome shotgun sequence.
ACCESSION   DBNGZC010000983 DBNGZC010000000
VERSION     DBNGZC010000983.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2628883"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1563)
                     /locus_tag="AC16G0_16950"
                     /pseudo
     CDS             complement(70..1563)
                     /locus_tag="AC16G0_16950"
                     /inference="COORDINATES: protein motif:HMM:NF018012.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
     gene            complement(1627..2796)
                     /locus_tag="AC16G0_16955"
     CDS             complement(1627..2796)
                     /locus_tag="AC16G0_16955"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF027964.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR5101345.1"
                     /translation="MRILVLGTGLQGRASLHHLDRSPTVSHVIAADADLAGLTRYLDR
                     LKTSKVEAVALDANNRGQVAALMRKVQAVIVLLPVSFHVPLTRLAVESGIQLVNSSYV
                     PPEFQEIGRDAAARGLAILPEFGFDPGIDLVLAGQAVKELDEVHEFYSYGAGFPEPRA
                     ATGPLRYKISWTFDGVLKSYMRDAHVIREGKTVAVSGRDIFAPSNIHTVNMEGYGALE
                     AYPNGDVVHYLDAMGVTATVRNAGRFAMRWSGHCAFWYALAQLGFLDHVPVQAGSASV
                     VPRDFVRLLLEPRLQYAEDERDVAVIRVDVRGMKNGRRRRLVYQVIDLRDLESGLLAM
                     NRTVGYTASIGVQMILRGDIQKRGLLSPLADIPADIFFRELRGCGIHVQREEGDW"
BASE COUNT          536 a         1004 c          893 g          540 t
ORIGIN      
        1 gatgccgagg tcgcagggag agccccggtc gtgccggcag gaccctccca tgccggtgac
       61 ctcttggact caccccagaa aatgcgcgta cttctttcgc agcgctgtca caatcgcggg
      121 gtccaccgga agcggagcgt gctcatgcag gatgcgcttg gccgcctctc tggcacgagc
      181 atacgtatcc ttggagccgt ccgactccca cttctcccgc ctctcccgat cgctgatggt
      241 atacggcgcg aactcggacc gcatatactt caccgtatgc tgctcgccca ggtagtcatg
      301 gcccgggccg acctccagca tgagatccag ggcgatcgtg tcgtcgttga cctcgatgcc
      361 gcgcaacacc cgatgggttc tggcaaggat ctcgttgtcg atcacgacct tttcatagga
      421 tgccgtgaga tcaaactcca tcaagcctgc ggcatcgtgt atgaagttcg cgcccgataa
      481 cgccaccagc agactcccca tcccgctctc ataaccggcc tgacaatcca ccagtttgga
      541 gtcggtcatc ccggcggtcg agtagtatgg taattgatag tgctgcgcca tctggctaca
      601 gccggcatgg atcatggccc gctcgattgc gccgctgagg tgcgccagag tgcgcatgtc
      661 cgcgatcgat cccacgctgc cgcagatcac cggcgttccc ttgttgaccg cctgcgtcag
      721 gacgactccc gcaagcgtct cagccgtgtg catcacgaca tttgccgcga gcgtcacggg
      781 cgacgtcaag ccgcccagcg gctctgtcgg gaccacgacc ggaagcccct tttcggcgag
      841 gtaacatgtg aagtccccgt agcgatcgtc gatcttgaag ggactgatca tcagcgtgat
      901 gaagctcacg aagggccgcg cccggagttg ctcagcgccc ccggccagca tctcggccat
      961 ctccaccact tcacgcagac cctgagatcc gtacatcccg ccctggacgt gcttgccggt
     1021 attgctgatg gcggcgtaga agcgattgag atccacatgc tccggctcgc ggatatcgtt
     1081 ggggtacacg ttgatcagaa agaaatgcac ccaatcgcag gcgtcgacca gccgtgccat
     1141 gtcccgcaca tcggccaggg tcgaggggcg ccgcgcgccg gtgtcgatgt ccaggacatg
     1201 gatggccgtc ccccccgtcc ccaggtacac attcggccct tcgaggacga tgtcctgccg
     1261 ggggtcgcga ccgcacaggg tgatgctgtg cggcgcggag gcgatggaat cctcaaccat
     1321 gtcgcgcggg attctcacga tgcaggagga ttcatcgacg gtcgctcccg cccgcctgag
     1381 atactcccgc cccacgcgtg tgaagacctt gacgccgccc ttctccagga gggtcatcac
     1441 cgcgtgatga atcttctgca catcgacgtc ggagaacggt ttgtactgtc cgccctttga
     1501 aggcatgctg aggacctccc cggtcatcgg gatgcgatag ccgcgtcgaa tctcgtccct
     1561 gccaggccgc ccgcgatgga gccggccatt accaccgccc cgctaccgtc gcccaatctc
     1621 caggacctac cagtccccct cctcacgctg cacatggatg ccgcacccgc gcaactccct
     1681 gaagaagatg tcggcaggga tgtctgcaag gggagagagg agccctcgct tttggatgtc
     1741 cccccggaga atcatctgga ccccgatgct ggccgtgtac cccaccgtcc ggttcatggc
     1801 cagcagaccg ctttccagat cgcgcaggtc aatcacctgg tacacgaggc gtcgcctccg
     1861 gccgtttttc atcccgcgca catccacccg gatcaccgcc acgtcccgtt cgtcttccgc
     1921 atactgaagc cggggctcca gaaggagccg gacgaaatct ctgggcacga ccgacgcgct
     1981 ccccgcctgg accggcacgt ggtccaggaa gcccaactga gccagagcgt accagaacgc
     2041 gcagtggccg gaccagcgca tggcaaagcg gcctgcattc ctgaccgtgg ccgtcacacc
     2101 catggcgtcg aggtagtgca ccacatcgcc gttgggatag gcctccaggg caccgtaccc
     2161 ttccatgttc accgtgtgga tgttggacgg ggcgaagatg tcgcgtcccg aaaccgccac
     2221 ggtctttccc tcacggatga cgtgcgcgtc ccgcatgtag gatttcagga cgccatcgaa
     2281 agtccaggag atcttatagc ggagggggcc ggtggccgcc ctgggctcgg ggaagcccgc
     2341 cccgtaggag tagaactcgt ggacctcgtc caactccttc accgcctggc cggccaggac
     2401 caggtcaatt cccgggtcga agccgaactc cgggaggatc gccaggccgc gtgcggcagc
     2461 gtcccgtccg atctcctgaa actcgggcgg cacataggag gagttgacca gctgaatgcc
     2521 gctttccacg gccagccgcg tgaggggcac gtggaaggag actggaagca ggacgatgac
     2581 cgcctgaacc ttccgcatca gcgccgccac ctggcctcga ttgttggcgt ccagcgcaac
     2641 cgcctcgacc ttggatgtct tcaggcgatc gaggtagcgc gtcagcccgg cgaggtctgc
     2701 atcggccgcg atgacgtggg agacggtcgg gctccggtcc aggtggtgca ggctggctcg
     2761 cccttgaaga cccgtgccca gcaccaggat tctcacggct tgatctcctc cccgtgggtg
     2821 tcgcgaatca gccttagaag gccagcagtt gcgtgagcga ggtgacgtcc ggcgtacgcg
     2881 gcagatcgaa gatggcggcc tgcagcctcc cgcaggcctt ctcgcccacg accttcgtcg
     2941 tcaacgagcg gaacttggcc ttcaggtcgt cat
//
LOCUS       DBNGZC010000984         1242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_474866, whole genome shotgun sequence.
ACCESSION   DBNGZC010000984 DBNGZC010000000
VERSION     DBNGZC010000984.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1242
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_474866"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="AC16G0_16960"
     CDS             complement(<1..313)
                     /locus_tag="AC16G0_16960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101346.1"
                     /translation="MLARRRTATLISVCSMLMVFGVVVFFDGSAEGRRKPGAAPKSSG
                     VEKAQDPEPAEATPDAETFLRTLHQAGIRLEGQSRDEILQDAADLFNRMQEVTPPPEV
                     TG"
     gene            complement(349..>1242)
                     /gene="bamJ"
                     /locus_tag="AC16G0_16965"
     CDS             complement(349..>1242)
                     /gene="bamJ"
                     /locus_tag="AC16G0_16965"
                     /inference="COORDINATES: protein motif:HMM:NF053689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BamJ family beta-barrel-assembly machinery
                     protein"
                     /protein_id="HIR5101347.1"
                     /translation="AMMISQTATRWAVVLALLCGIAAAPRPAGAAATPTQETPPEMQA
                     ESEAAAVDKGGAPSQGPQEGAPFKNWKEKLSYSLGVDLASNLRWQRIDVDVDLLVEAL
                     RDTLAGKKLLLTGEEVLATVQRFEAERKHDFEHAKAMRAERNKAQGEAFVAQNVKKDG
                     VVILPSGLQYKILKQGDGKKPTRDDKVVVQYRGTLLDGTEFDSSSKRNGPVTVPVKGL
                     IKGWSEALQLMPVGSKWQIFIPSQLAYGEKVVAGIGPNALVIFEVELLSIADSSGDGA
                     QPSATDTKTSRLRTETRPASR"
BASE COUNT          183 a          423 c          366 g          270 t
ORIGIN      
        1 ccccggtgac ttcgggtggc ggtgtcacct cttgcatccg attgaagagg tctgccgcgt
       61 cttgcagaat ttcatccctg ctttggcctt ccagccttat gcccgcctgg tgcagtgtcc
      121 tgaggaaggt ctctgcgtcc ggggtggcct cggccggttc gggatcctgc gctttctcca
      181 cacccgagga ttttggagcg gcgcctggct ttcgacgccc ctccgcgctt ccgtcgaaga
      241 acaccaccac gccgaagacc atcagcattg aacagacgct gatgagcgtt gccgtacgtc
      301 tccttgccag catgggtatt tccccttccg tccactcctc tggggcggct acctggaagc
      361 cggtctggtc tcggtcctca atcgcgatgt cttcgtatcg gtggccgaag gttgggcgcc
      421 atccccggag gagtccgcaa tggaaagcag ctccacctcg aagatgacca aggcgttggg
      481 cccgatccca gccactacct tctctccata cgccagttgc gacgggatga agatctgcca
      541 cttggagccc acaggcatga gctgcagcgc ctcggaccag cccttgatga gacccttgac
      601 gggcacggtg accggtccgt tccgcttgga ggagctatcg aactctgtcc cgtccaggag
      661 cgtgccccga tactggacga cgaccttgtc gtcacgggtg ggcttcttgc cgtcgccctg
      721 cttcaggatc ttgtactgga gaccgctggg caggatcacc acgccgtcct tcttgacatt
      781 ctgcgcgacg aaggcctctc cctgtgcctt gtttcgttcc gcccgcatgg ccttggcgtg
      841 ctcgaagtcg tgcttccgct ccgcctcgaa ccgctgcacg gtggcgagca cctcctcgcc
      901 ggtcagcagt agcttcttgc ccgccagcgt gtcgcgcagg gcctcgacga gcagatccac
      961 atccacgtct atccgctgcc accggagatt actggccaag tcaaccccca gcgaatagct
     1021 cagcttttcc ttccagttct tgaagggcgc gccctcctgg ggcccctggc tgggtgcgcc
     1081 ccctttgtcg actgctgcag cctccgattc ggcctgcatc tcggggggcg tttcttgggt
     1141 tggggtcgct gccgctccgg cgggccgcgg agccgcggcg atcccgcaga ggagcgccaa
     1201 aaccactgcc catcgggttg ccgtctggga gatcatcatt gc
//
LOCUS       DBNGZC010000985         1742 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_571716, whole genome shotgun sequence.
ACCESSION   DBNGZC010000985 DBNGZC010000000
VERSION     DBNGZC010000985.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_571716"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="AC16G0_16970"
     CDS             <1..324
                     /locus_tag="AC16G0_16970"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR5101348.1"
                     /translation="LTEAEFKMRFRGSPILRAKRRGFLRNICVALGNLKRPEAVTALV
                     KTLREDPEALVRAHAAWALGQIGTEEAKAAIREAARQEADPSVLAEIRAARRGLAAVD
                     GPCLE"
     gene            415..>1742
                     /locus_tag="AC16G0_16975"
     CDS             415..>1742
                     /locus_tag="AC16G0_16975"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018195989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HIR5101349.1"
                     /translation="MNYPIWDLPILGGGVLIGTIAILHVFVSHFAVGGGLFLVLTERK
                     AYRENDPDLLAYLKTHSTFFVLVTLVFGAVSGVGIWWSIGLVAPAATSTLIHVFVWVW
                     AMEWVAFVTEIAAAFVYYYGWNRLDRRTHEIVGWIYFGAAWLSLFQINGILTFMLTPG
                     RWLQTSSMLDGFFNPTMWPSALIRTAVAVALAGLYALVTAAVGSPQALRERLTQYAAK
                     WLVLGAAVIPVGGIWYIGSLPPLAREISMGGAPAVTIFAGLTIVFSGLLVALVLFSAY
                     LYPRSFNIGLALLAVILGLMTTGVTEWVREAVRKPYIIYGYMWSNSIRVAEAKAFEQT
                     GLLSRAKWVAESEAAPSPRSRVGMEVFQLACQSCHTIEGYNGIRIMVKGWPRALIRYS
                     LDHLDELKGFMPPFVGNTTEREALADWLYALGRPASASAARAMAGRFPVPP"
BASE COUNT          253 a          541 c          613 g          335 t
ORIGIN      
        1 ctgaccgagg cggaattcaa gatgcgcttc cggggaagtc cgatcctgcg ggcgaagcgt
       61 cgagggttcc tgcggaacat ctgcgtggcc ctcggaaact tgaagcgccc ggaggccgtt
      121 accgccctgg tcaagacgct ccgggaggac ccggaggccc tggtccgggc ccacgccgcc
      181 tgggcgctgg ggcagatcgg gacggaggag gcgaaggcgg cgatccggga agccgccagg
      241 caagaggccg acccctcggt tctggcagag atccgggcag cccggcgtgg attggcggcg
      301 gttgacgggc cgtgcctgga ataggtagta tctccccctg tgtgaacgag agcacgagcc
      361 cgcggcctgg ggtgccggcc gacgcaattc cctgcatgtg atgggggtct gtcgatgaac
      421 tacccgatct gggacctccc gatcctgggc ggtggggtgt tgatcggcac catcgccatc
      481 ctgcacgtct tcgtttccca cttcgccgtg ggcggagggc tttttctcgt cctcacggag
      541 cgcaaggcgt accgggaaaa cgaccccgac ctcctggcgt acctgaagac ccatagcacg
      601 tttttcgtcc tcgtgaccct ggtcttcggg gccgtgagcg gtgtgggaat ctggtggagc
      661 atcggcctgg tggcaccggc ggccacctcg accctgattc atgtgttcgt gtgggtctgg
      721 gccatggaat gggtggcgtt cgtcaccgag atcgccgccg ccttcgtcta ctactatggc
      781 tggaaccggc tcgaccggcg gacccacgag atcgtcgggt ggatctattt cggcgcggcg
      841 tggttgagcc tgtttcagat caacggcatc ctcaccttca tgctcacgcc gggtcggtgg
      901 ctgcagacca gctccatgct ggacgggttc ttcaatccga cgatgtggcc atcggcgctc
      961 atccggaccg cggtggcagt ggcgctggcc ggtctgtacg ccctggtgac ggccgcggtc
     1021 ggctccccgc aggccctccg cgagcggctg actcagtatg ccgcaaagtg gctggtcctg
     1081 ggggcggcgg tcatcccggt ggggggcatc tggtacatcg gctcgctgcc gccgctggcg
     1141 agagagatct ccatgggcgg ggccccggca gtcacgatct tcgcgggtct cacgatcgtg
     1201 ttctcggggt tgcttgtcgc cctggtcctt ttctccgcct atttgtatcc gcggagcttc
     1261 aacatcggcc tggctctcct cgcggtcatc ctcgggctaa tgaccaccgg cgtcaccgag
     1321 tgggtccgag aggcggtgcg gaaaccctac atcatctacg gctacatgtg gtccaactcg
     1381 atccgggtgg cggaggcaaa ggcattcgag cagactgggc tcctgtcgcg ggcgaagtgg
     1441 gtcgcggaga gcgaggcggc gccgtcccct cggtcccggg tcggtatgga ggtcttccag
     1501 ctggcgtgcc agagctgcca tacgatcgag ggatataacg gcatccggat catggtgaag
     1561 gggtggccgc gggcactcat ccgatacagc ctggatcacc tggacgagtt gaagggcttc
     1621 atgccgccct tcgtgggcaa cacgaccgag cgggaggccc tggcggattg gctgtatgcg
     1681 ctcggccggc ccgcctcggc ttctgcggcg cgggcgatgg ctggccgttt cccggttccg
     1741 cc
//
LOCUS       DBNGZC010000986         1856 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2431924, whole genome shotgun sequence.
ACCESSION   DBNGZC010000986 DBNGZC010000000
VERSION     DBNGZC010000986.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2431924"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1080
                     /locus_tag="AC16G0_16980"
     CDS             127..1080
                     /locus_tag="AC16G0_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5101350.1"
                     /translation="MPEFVSQVAAKIKGELGKVVVGQEAVIEQILVALLAQGHVLLEG
                     VPGTAKTLMAKCLAHILGVGFNRVQFTPDLMPSDVLGTSVFEPGSGTFRLRTGPIFTE
                     ILLADEINRTPPKTQAALLEGMEERQVTIDGQRHPLPPLFLVLATQNPVEYEGTYPLP
                     EAQLDRFFMKVAIGYPSEAEEQQVVLRHHGGFDPRNLDQAGLARFKDREVVLRCREHV
                     QKVSVEEGISRYTVQIARASRETPACVMGASPRASVALLVAAKCLAAIRGKTYVTPDD
                     VKSVAAPVLRHRLILKPEAEIEGYTADRVVEDLLAQIPVPR"
     gene            1180..>1856
                     /locus_tag="AC16G0_16985"
     CDS             1180..>1856
                     /locus_tag="AC16G0_16985"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR5101351.1"
                     /translation="MQVRLIPTPRLVFLFALGMLPLALVAQVPEVRWGVGFFDVVLLL
                     LVATDFQRAPGAAVLLVERRVGPRLAIGEHETVTLLVRSATPHDLVVELRDEPPLDFL
                     SAGRLLTLRVPPLGRAQGSYSVTAPARGDFAFAGFTARVPGPLGLVRRQWRSPHRTEV
                     QVYPNFRLAARMELLGRRSHLLQTGLHSLRRRGEGRAFESLRDYVQGDDTRHIDWKAT
                     ARRRKPIV"
BASE COUNT          289 a          601 c          646 g          320 t
ORIGIN      
        1 acgtcggatg aggcccttct gaagtttgcc cagacggtgc accgcctcga ggggcgcctg
       61 cggctccggc cggtccacgg agcggcaggc gggatgcgaa agcctcacta gaccaacgga
      121 gggacgatgc cggaattcgt gagtcaggtg gcggcaaaga tcaagggtga attgggtaaa
      181 gtcgtcgtgg ggcaggaggc ggtcatcgag caaatcctgg tggccctcct ggctcagggc
      241 cacgttctgt tggagggtgt ccccggaacg gcgaagaccc tgatggcgaa atgcctggcc
      301 cacatcttgg gagtcgggtt caaccgggtc caattcacgc cggatctgat gccctcggac
      361 gtgctgggca cgtcggtgtt cgaaccgggg tccggcactt tcagactacg caccgggccc
      421 atcttcaccg agattctcct ggccgacgag atcaatcgga cgccgcccaa gacgcaggct
      481 gccttgctcg agggcatgga ggaacgacaa gtcaccatcg acggccagcg ccacccgctc
      541 ccgcccttgt tcttggtgct ggccacccag aacccggtgg agtacgaggg aacgtacccg
      601 cttcccgagg cccagctcga ccggttcttc atgaaggtgg ccatcgggta ccccagcgag
      661 gcggaggagc agcaggtggt cttgcgccac cacggggggt tcgacccgcg caacctcgac
      721 caggccggcc tggcgcgctt caaggatcgg gaggtggtcc tgcgctgccg cgagcacgtc
      781 cagaaggtca gcgtggagga ggggatctct cgctacacgg tgcagatcgc ccgcgccagc
      841 cgcgagactc cggcgtgcgt gatgggggcg agccccaggg cctcggtcgc cctgctggtg
      901 gcggccaagt gcctggctgc aatccggggg aagacctatg tgacacccga tgatgtgaag
      961 agcgtggccg cgccggtgct ccgccaccgc ctgatcctga agcccgaggc ggagatcgag
     1021 ggctacacgg cggaccgggt ggtggaggat ctgctggcac agattcccgt tccccgctga
     1081 tcgccggagc gaccggcagg agcgatcagt gccgaatcct ggccctctgc ttggctcgaa
     1141 gctgacagcc attggttgac tgctgaaggt acggttccca tgcaagtccg tctgatcccg
     1201 acgccccgcc tggtgttctt gttcgccctg gggatgcttc ccctggcgct ggtcgcccag
     1261 gtgccggaag tgcgctgggg tgtgggattc ttcgatgtgg tcctgctact cctcgtcgcg
     1321 acggatttcc agcgggcacc gggcgccgcc gtgctcctgg tcgagcggcg ggtggggccg
     1381 cgcctggcca tcggcgagca tgagacggtc acccttctgg tccgcagtgc gaccccgcac
     1441 gacctggtgg tggagctacg cgatgagcca ccgctggatt tcctctcggc gggacgcctc
     1501 ctcaccctcc gtgtaccgcc gctgggtcgg gcacagggct cctattcggt cacggcaccg
     1561 gcccgtgggg atttcgcctt cgctgggttc accgcccgtg tgccaggccc gttgggcctg
     1621 gttcggcgcc agtggcgctc cccgcaccgg accgaggtgc aggtgtatcc caatttccgc
     1681 ttggcggcga ggatggagct gctgggccgc cgctcccacc tcctccagac gggcctccac
     1741 tccctgcggc gacgggggga ggggcgagcg ttcgaatccc tccgggacta cgttcagggg
     1801 gacgacactc gtcacatcga ttggaaggcc acggcccgac ggagaaagcc catcgt
//
LOCUS       DBNGZC010000987         4633 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_475418, whole genome shotgun sequence.
ACCESSION   DBNGZC010000987 DBNGZC010000000
VERSION     DBNGZC010000987.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_475418"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="AC16G0_16990"
     CDS             complement(<1..925)
                     /locus_tag="AC16G0_16990"
                     /inference="COORDINATES: protein motif:HMM:NF012385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR5101352.1"
                     /translation="MPPLVLVVEDEVILGDSISVYLERHGYATSVARSGEDALRLAET
                     TSPDVAVMDIRLPGMDGMEVLRRLRELSPGTEVVMMTAHASVASAVEAMRRGAFDYLT
                     KPLDLDELLLAIEKALSHQRMRQEWSYLKARDETGGHLAEILGESPGMRTLRQQIARI
                     AALDAPGSGDAPTVLILGETGVGKEMVARALHYTSPRASGPFVEINCAAIPTSLLEAE
                     LFGSEKGAYTDARAAKPGLFEAGDGGTVFLDEIGHMDHVLQVKLLKAIEEKAVRRVGG
                     LRTKPFNARIGAATHRDLEAAIAGGTFRPDLY"
     gene            complement(929..2374)
                     /locus_tag="AC16G0_16995"
     CDS             complement(929..2374)
                     /locus_tag="AC16G0_16995"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5101353.1"
                     /translation="MPIERSHPGKTLIHQFALLSFLVIGLITLALSLLIGYNLRKDLL
                     DREWGTTADFIRTEAQQRLVLADFLAPMTPSAQAHFEQLYHETVMMPEIVRVKIYDGT
                     GTVIWSDEPRLRGQSFPDNFHLMQALSGRTTINLDTDEAKDENLYERNAFAQLVEVYV
                     PIVFPGSSRVVGVVESYKMPTQVIASIRRGQLTVVSTAVAGGIMLYLSLFWIVRRAGR
                     RLGEQHQDLAQRTQELTAANQELRAVQAQLLEAERLAAIGEVVTAVAHGIRNPLANIR
                     ASAQVAGLDCGACQASKIGPRNLANIMAEVDRLEGRLKELLQFVRPAERPCACLDLNT
                     VLGDALQLMGGRLAKAPLRLIQELAPNLPPFRGNAMLVEQVFLSLLGNALEALPNGNG
                     TITVRTGTTEDAVGTPRLFAEVQDSGSGIPPGQLSKIFEPFYTTKAQGTGLGLAIAKK
                     FTEAQAGTIEVSSPSAGGAAFRVTFPVAQEV"
     gene            complement(2752..3567)
                     /locus_tag="AC16G0_17000"
     CDS             complement(2752..3567)
                     /locus_tag="AC16G0_17000"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5101354.1"
                     /translation="MLGFDGAHGGRKGKAVVLFGLLLVILLTGYSSTLAKAIPRQWES
                     SQAERSYTEGLDRYKAKDYSGAIPLLQRALELEPEFDDAESYLGWSLYRIGRYPEATL
                     HFRQVILRQPGRAEAYDGLGWSRFQVNRYNLARAAVRPAQAQDPRHRDARVGYAFSLF
                     ELARYVEALPQLERLIQEGEGTAPSRSLPDVEKVRARLAWALFYLGDYRRAREQFLKG
                     LEAQPDWAGLHNGLAWTFLRLGDRPQALASFRRALELQPDFADALDGLTQAGR"
     gene            3739..4050
                     /locus_tag="AC16G0_17005"
     CDS             3739..4050
                     /locus_tag="AC16G0_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101355.1"
                     /translation="MARVTVTLSRLVRGILASAPTSALRTTAPTLTIRARRTRIPPAP
                     AAGGIASAAYASIAVAATAAIIATPAASTILRIGDTGLPMPGDELQVHQKWHDHQRQK
                     H"
     gene            complement(4419..>4633)
                     /locus_tag="AC16G0_17010"
     CDS             complement(4419..>4633)
                     /locus_tag="AC16G0_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101356.1"
                     /translation="LGQNRGRDRDRGMEARDQNEAAEEAAEAQAEAAREANENQMGAA
                     EAQARGQVEARGRAAEGEAPRGQDPR"
BASE COUNT          818 a         1568 c         1382 g          865 t
ORIGIN      
        1 agtagagatc gggacggaag gtcccgcctg cgatggcagc ctccagatcc cggtgcgtcg
       61 cggcgccgat gcgggcgttg aagggcttcg tccgaagccc ccccacccgg cgcaccgcct
      121 tctcctcgat cgccttgagg agcttcacct ggaggacgtg gtccatgtgg ccgatttcat
      181 ccaggaagac ggtgccgccg tcgcccgcct cgaacagacc gggcttggcc gcccgggcgt
      241 ccgtgtaagc acccttctca gagccgaaaa gttccgcctc cagcagcgag gtaggaatgg
      301 ccgcgcagtt gatctccacg aagggtcccg aagccctggg gctcgtgtaa tggagcgccc
      361 gggcgaccat ctcctttccg acccccgtct ctcccaggat caagacggtc ggggcatccc
      421 ccgagccggg ggcatccagc gccgcgatgc gtgcgatttg ctgccgaagg gtgcgcattc
      481 cgggggattc acccaggatc tcggcgaggt gaccgcccgt ctcatctcgg gccttcagat
      541 acgaccattc ctgccgcatc cgctggtggg acagggcctt ttctatcgcc agaaggagtt
      601 catccaggtc gaggggtttc gtcaagtagt cgaaagcccc ccgtctcatc gcttcgaccg
      661 ccgatgcgac ggaggcgtgg gccgtcatca tgacgacctc ggtccccggt gacagctcgc
      721 ggagtctccg gaggacctcc atcccgtcca tccctgggag tcggatatcc atcaccgcca
      781 catccgggct cgtggtctct gcaagtcgga gggcatcttc gccggagcgg gctaccgacg
      841 tggcgtagcc atggcgctcc agatagacgg agatggaatc ccccaagatg acctcatcct
      901 caaccaccag caccaggggc ggcatgtctc agacctcttg ggcgacgggg aacgtgacgc
      961 ggaaggcggc gccgccggcc gatgggctcg agacctcgat ggtcccggcc tgggcctcgg
     1021 tgaatttctt ggcaatggcc aggcccagcc cggtcccttg cgccttggtg gtgtagaagg
     1081 gctcgaagat cttcgaaagc tggccgggcg gaatcccgct tccgctgtcc tgaacttccg
     1141 cgaagagtct gggagtcccg accgcgtcct ccgtcgttcc agtgcggacc gtgatggtcc
     1201 cattgccgtt gggcagggcc tccagggcgt tccccaggag actcaagaag acttgctcca
     1261 cgagcatcgc gttccctcgg aacggcggca ggttgggagc aagttcctgg atgagacgga
     1321 gcggggcctt ggccagacgc ccccccatga gctgcaatgc gtcgcccaga accgtgttga
     1381 gatcgagaca ggcacacggg cgctccgccg gccggacaaa ctgcaataac tccttcaggc
     1441 ggccctccag cctgtccacc tcggccatga tgttggcaag gttcctcggc ccaattttgg
     1501 atgcctgaca tgccccgcag tccaggccgg ccacttgagc cgaggctcgg atattggcca
     1561 gcgggttgcg gatgccgtgg gccaccgccg tcaccacctc gccaatggcg gccaggcgct
     1621 ccgcttccag gagttgcgcc tgcacggccc ggagctcctg gtttgcggcg gtgagttcct
     1681 gggtccgttg ggccagatcc tgatgctgct ctcccagcct cctgccggcg cgcctgacga
     1741 tccagaagag cgagaggtac agcatgatgc cgccggcgac ggcggtgctg acgaccgtga
     1801 gctggccgcg tcggatgctc gcgatgacct gggtgggcat cttgtaactc tcgacgacgc
     1861 cgaccacacg ggagctcccc ggaaagacga tgggcacgta cacctcgacg agttgggcaa
     1921 aagcattccg ctcgtagagg ttctcgtcct tcgcctcgtc cgtgtccaga ttgatcgttg
     1981 tccgcccgga aagcgcctgc atgaggtgga agttatccgg aaagctctgg ccacggagac
     2041 gcggctcgtc ggaccagatg accgttcctg tgccatcata gatcttcaca cgaacaatct
     2101 cgggcatcat gacagtctca tgatacagtt gctcgaagtg tgcctgggcg ctcggggtca
     2161 ttggtgccaa gaaatctgct agaaccaggc gttgttgggc ttcggtgcgg atgaagtccg
     2221 cggtcgtgcc ccattcccgg tccagcaggt ccttccgcaa gttgtagccg atgagcaggg
     2281 atagagccag cgtgatcagc ccgatgacga gaaagctcag gagggcgaac tgatggatca
     2341 gcgtcttgcc cgggtgcgac cgctcgatgg gcaacgaccg tgttccggga gaggcctggg
     2401 agaccgcctc agatccgatg gtgagcaggc ctgccggagt gagggacaat cttcccccgc
     2461 cctctgaaac tttcggggct ctgaaagagg ggaaggccgc caattcgggc gaggtcatca
     2521 cccgttgcat tccccgcccg gctatttcct ggttcatctg tctcatcgcg acaccccgtt
     2581 cccacacgca gaacactccg ttgggaagcg ccacctcaca atttcgcgca gggtcagtcc
     2641 gaaggcaacc gtcgaccttt tcggtcgaga tccaaccctc gctcttcggt gtactgcctg
     2701 cccgtcaatt cacccccggc gcttcgggtg aaggtcgaac cgggttgggg ctcaccgccc
     2761 cgcctgggtg aggccgtcca gggcatctgc aaaatccggc tgcagttcca gtgcgcgccg
     2821 aaagctcgcc aacgcctggg ggcggtcacc cagacgaagg aaggtccacg ccaggccatt
     2881 gtgcaagccc gcccagtcag gttgggcctc caggcccttg aggaactgct ccctggcacg
     2941 ccggtagtcc ccgaggtaga agagagccca ggccaagcgg gcccggacct tctcgacatc
     3001 cgggagagat cgcgaagggg cagtcccctc accctcttga atcaggcgct ccaactgcgg
     3061 aagggcctcc acgtatcgag ccaactcgaa cagggaaaat gcatagccca ccctggcatc
     3121 tctgtggcgt gggtcctggg cctgggccgg ccggaccgcc gcccgcgcca ggttgtaccg
     3181 gtttacctga aagcgactcc atcccagtcc gtcgtacgcc tccgccctac ccggttggcg
     3241 gagaatgact tgacggaagt ggagggtggc ctcgggatat ctgccgatgc ggtaaagact
     3301 ccatcccagg tacgactcgg catcatcgaa ctccggctcc aactcgaggg cacgttgaag
     3361 caaggggatc gcacccgaat aatccttcgc cttgtaccga tcgagtccct ctgtgtagct
     3421 ccgctcggcc tgggagctct cccattgtct gggaatagct ttcgcaaggg tgctggaata
     3481 acccgtcaac aagatgacga gtagcaggcc gaaaaggact accgctttcc ctttgcgccc
     3541 tccatgcgcc ccatcaaacc cgagcatgag ccacacccct cctgtctatc tcggcgtggt
     3601 ggcgttggca atcaaggcga gctcacgatc ttcggcttcc gcctcgcggt agttcgcttc
     3661 catctcgtgg gcccggaccg aggccccgcg attcatgccc cggccctgat cctggcctga
     3721 tgccggactt ccgacgccat ggcccgcgtg accgtgaccc tttccagact cgtgcgcggc
     3781 atcttggcct cggctcccac ctcggccctg cgcacgaccg cccccaccct cactatccga
     3841 gccaggcgca cccgcattcc ccccgcgccc gccgccggag gcatcgcgtc cgccgcttac
     3901 gccagcattg ccgtcgccgc cactgctgcc atcattgcca cccccgccgc ctccaccatc
     3961 cttcgcatag gcgataccgg tctgcccatg cccggggatg aactgcaggt tcaccagaaa
     4021 tggcacgatc atcagaggca gaagcattga catcaggcgc tccaagagcc tgttcatcgg
     4081 gtcgtcactc ctcgcaacat tacagccaag ttcgacagca ctcgtgagga aatacttggt
     4141 gggcagaaaa tcttcaccct ccacaatcgt catataacgc catcggttcc cctaaatccg
     4201 aaaaaacctg aggatttgag agatatggca acccttcctc aacctcaagt agcctttcga
     4261 cctcttggtg cgcctgctga gctttttcgc agaaatccta aggaagagag ggggcatggg
     4321 atttcccatg cccccaccat cggctttcaa ggattgtcgc gagattctga ggcgctgact
     4381 tcgtccagcc tcgcgacctt gttcgttgct cccacctcct agcgcggatc ctggccgcga
     4441 ggggcttctc cctcggctgc ccggccgcgg gcctcgacct gaccccgcgc ctgcgcctca
     4501 gcggccccca tctggttctc attagcctcg cgagcggcct ctgcctgggc ctcagcagcc
     4561 tcttcggcag cctcgttctg atcgcgggcc tccatccctc gatcgcgatc ccgtccgcga
     4621 ttctgcccca gat
//
LOCUS       DBNGZC010000988         3637 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_279990, whole genome shotgun sequence.
ACCESSION   DBNGZC010000988 DBNGZC010000000
VERSION     DBNGZC010000988.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_279990"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1710
                     /locus_tag="AC16G0_17015"
     CDS             <1..1710
                     /locus_tag="AC16G0_17015"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017721873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR5101357.1"
                     /translation="TRAHEYGGGAYLVAEGVVYFSNFADQRLYRHRPGRGGTPEPVTP
                     AGNDCYADGIWDRRRRRILSVREDHTGGGEPANTLVAVDAERGGPGEILLSGRDFYMA
                     PRLDPDGTRLAFLAWDHPNMPWDAAEVWVASVRADGSLAAPEHIAGGKGDSAIQPNWS
                     PEGILHFVSDRTGWWNLYRVAAGRVEALTQLEGEFAQPPWVFGMSHHAFARRLVSAYS
                     QGGMWKLATVDPKARRLDPVGMPYTGFASVHSDGKWVALLAGSPVEPWSVIRLDPDSG
                     QQEIIRRATELRFDPGYLSTPLPIEFPTAGARTAHGIFYAPRNKDFVAPERERPPLLV
                     MIHGGPTSATTTVLRLGIQYYTSRGFAVLDVNYGGSTGYGREYRERLYGQWGVVDVDD
                     CCHGALYLAAQGLVDPTRLAITGGSAGGYTTLACLAFRDVFAAGASHYGVSDCEILVK
                     ETHKFEARYLYTLIGPYPERRDLYLERSPLHHLESLRRPVIFFQGLDDKVVPPNQAEM
                     MWSALRERGVPTAYVPFPGEQHGFRRAENIRRALEGEFYFFSRVFGFEPADAIQPVPI
                     ENL"
     gene            complement(1796..>3637)
                     /locus_tag="AC16G0_17020"
     CDS             complement(1796..>3637)
                     /locus_tag="AC16G0_17020"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR5101358.1"
                     /translation="EVVRFAREHSYLRFPGELARAVPATPPPADVSTLTIADQQAQLD
                     RMLQDLQTMTARSVFRAYGYVPAHGSPLTLFTSMFLHGGWLHLFGNMLFLWLAGTSLE
                     DRWGRVIFLVLYLASGAIATLTHASLNPESTVPMVGASGAIAGLMGAFLVRLATTRIR
                     FFYWIYFIQGTFSAPAYVVLPLWLLQQFAMAGSGQAGGVAVWAHIGGFAVGVAAAFLI
                     LGTELEEKFLAPVIQKKTTWTVSERMNAALNRLDRGEVDRAITELQALLRLNPESIEA
                     RTPLIAAYEQKGDHAAAGIESARLVGGYVWMRDMEGALTAAQEHDRSYRDVPLFMRDL
                     LALAAHREKHEQYAEAAQLYQRAIAAWPDDPLTPKALLAYGQSMLQNFREPGGALKIL
                     EQAQAHPKATPEFLKASEALIAEARQALGAEGQPHQVAPSPAPELSVSEPAAEPPAEA
                     AAEEVPVEVPPAPVPDRSLVAVPMRAVGIDGRGLQLQSRSGKLGRLPWQQVAAVSVAS
                     IGVYNASDPIAASLLLDLLMRPKSTPAGEVVHSVRLSIQDLAIPQLQAESSSVRAFQR
                     FVATILKATGAPPHPSRDACLGLGGFPAYPDLADYEAELLLGLPAAE"
BASE COUNT          619 a         1172 c         1245 g          601 t
ORIGIN      
        1 acgcgggctc acgagtacgg cggcggggcg tacctcgtgg ccgaaggtgt agtgtatttt
       61 tccaatttcg ccgatcagcg gctgtaccgt caccggccgg ggcgaggcgg cacgccggag
      121 cccgtgacgc ccgccggcaa tgactgctat gccgacggca tatgggaccg gcgccggagg
      181 cgaatcctct ccgtccggga ggatcatacc ggcggcggcg agccggccaa caccctcgtg
      241 gccgtggatg ccgagcgtgg tggcccgggg gagatcctcc tcagcggcag agatttctat
      301 atggcccccc gcctcgaccc cgacggcacg cgcctggctt ttctcgcctg ggatcacccg
      361 aacatgccat gggatgccgc ggaggtctgg gtggcgtccg tgcgagcgga cgggagcctg
      421 gccgcaccgg agcacatcgc gggcggaaag ggggattccg ccatccagcc gaactggtcg
      481 ccggaaggca ttctccactt tgtctccgat cggaccggct ggtggaacct ctatcgcgtt
      541 gcagccggcc gggtcgaggc ccttacccag ctcgagggcg agttcgcgca gccgccttgg
      601 gtgttcggca tgagccacca cgccttcgca cgtcgcctcg tctccgctta ctcccagggc
      661 gggatgtgga agctcgcgac cgtagacccg aaggcccgcc ggctcgatcc tgtgggcatg
      721 ccatacacgg gtttcgcgtc cgttcactcc gatgggaaat gggtcgcgct cctggccggc
      781 tctccggtcg agccgtggtc agtcatcaga ttggatccgg acagcggaca gcaggagatc
      841 atccgtcggg ccacggagct tcggttcgat ccgggatacc tctccactcc tttgccgatc
      901 gagtttccca cggcgggagc caggacggcc cacgggatct tttacgcgcc gaggaacaag
      961 gacttcgtcg cgccggagag ggagcgcccg ccgcttctgg tcatgatcca cggggggccg
     1021 acctcggcca ctaccacggt cctccggctg ggcatccagt attacaccag tcgcgggttc
     1081 gccgtgctcg acgtgaacta tggcggcagc acggggtacg gccgcgaata ccgggagcga
     1141 ctgtatggac agtggggcgt cgtcgacgtg gacgactgct gccacggcgc gctctacctg
     1201 gcggcacaag ggctcgtgga cccaacgcgt ctggcgatca ccggggggag cgccggcggg
     1261 tatacgacgc tcgcctgcct ggccttccgc gacgttttcg ccgccggcgc cagccactac
     1321 ggcgtgagtg attgcgagat cctggtaaag gagacgcaca agttcgaggc ccgctacctg
     1381 tacacactca ttggccccta cccggagcgt cgcgatctgt acctggagcg ctcgcccctc
     1441 caccatctgg aaagtctccg acggccggtg atcttcttcc agggtctgga tgacaaggtg
     1501 gtcccgccaa accaggcgga gatgatgtgg agcgccctgc gagagcgggg cgtccccaca
     1561 gcctacgtgc ccttccccgg cgagcagcac ggcttccgga gggcggagaa catccggcgg
     1621 gcgctggagg gggagttcta tttcttctct cgcgtcttcg gtttcgagcc ggccgatgcg
     1681 atccagcccg tgccgatcga gaatctgtga gcggtgagtc tgatcccctc ccaatggagg
     1741 agagagacgc gatgctgtca cgccagctcg aaccgccaga acggcggata cgggatcact
     1801 cggcggcggg tagaccaagc agaagctcgg cctcgtagtc ggcaaggtcc gggtacgcgg
     1861 ggaaaccgcc aaggccgagg caggcatcgc gactcggatg ggggggcgcg cctgtcgcct
     1921 tgaggatagt cgccacgaac cgctggaagg cgcgaacgga agaggattcc gcctggagct
     1981 gcggaatggc caggtcctgg atggagaggc ggacggagtg gaccacctca cctgcggggg
     2041 tggatttggg ccgcatgagg agatccagga gcagtgaagc cgcaatcgga tcgctcgcgt
     2101 tgtacacgcc gatgctcgca acggagacag ccgccacctg ctgccacggg agacggccca
     2161 atttccccga gcggctttgc aactgcaggc cgcggccgtc gatgcccacc gcccgcatgg
     2221 gcaccgccac cagcgagcga tctggcacag gagcgggagg gacctccacc gggacctctt
     2281 ccgccgcggc ctccgctggc ggctccgcag caggctccga gacggacagc tcgggcgcag
     2341 gcgagggagc cacctggtgt ggctgtcctt ctgcgccaag cgcctgtcga gcctcggcga
     2401 tcagcgcttc gctggccttc aggaattccg gcgtcgcctt ggggtgagcc tgggcctgct
     2461 caaggatctt cagggcaccg ccgggctccc ggaagttctg gagcatcgac tgcccatagg
     2521 cgaggagcgc cttgggggta agaggatcgt cgggccaggc ggcgatcgct ctctggtaga
     2581 gctgcgccgc ctcggcgtat tgttcatgct tctcccggtg ggccgccagg gccagcaggt
     2641 cccgcatgaa gagcggtacg tcccgatagc tacgatcgtg ctcttgggcg gcggtcaggg
     2701 ccccttccat gtctcgcatc catacgtagc cgcccaccag cctcgccgat tctatgcccg
     2761 cggcggcatg atcccccttc tgttcgtagg cagcgatcag gggcgttcga gcctcgatgc
     2821 tctcgggatt gagccggagc agtgcctgga gctccgtgat agccctgtcg acctcacctc
     2881 ggtcgaggcg gttcaaggct gcgttcatcc gttcggatac cgtccaggtg gttttcttct
     2941 ggatgacggg cgccaggaac ttctcctcca actcggtccc caggatcaga aaggccgccg
     3001 cgaccccaac ggcgaagccg ccgatatggg cccaaacggc cacgccccct gcctggccgc
     3061 tgcctgccat ggcgaactgc tgcaagagcc agagggggag gaccacgtag gccggcgcag
     3121 agaatgtgcc ctggatgaag tagatccagt agaaaaaccg gatgcgcgtc gtcgccagcc
     3181 ggaccaagaa tgcccccatc aacccggcga tggccccgga ggctcccacc atggggacgg
     3241 tgctctccgg attcaggctg gcgtgggtca gggtggctat ggcgccgctt gccagataca
     3301 gaacgagaaa tatgacgcgt ccccacctgt cctccaggct ggttcctgcg agccatagaa
     3361 agagcatgtt cccgaagagg tgcagccacc ccccatgcag gaacatcgag gtgaacaggg
     3421 tgagagggct tccgtgtgcc gggacatagc cgtaggcccg gaacacagag cgcgcggtca
     3481 tcgtctggag atcctggagc attcgatcca actgggcctg ctggtcggcg atagtcaggg
     3541 tagaaacatc ggctggcggg ggcgtggctg ggacggcgcg cgcaagctcc ccggggaagc
     3601 ggaggtagga gtgctcgcgc gcgaagcgca cgacctc
//
LOCUS       DBNGZC010000989         2945 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2432432, whole genome shotgun sequence.
ACCESSION   DBNGZC010000989 DBNGZC010000000
VERSION     DBNGZC010000989.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2432432"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..108
                     /locus_tag="AC16G0_17025"
     CDS             <1..108
                     /locus_tag="AC16G0_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002900913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101359.1"
                     /translation="IGKYVINHSFILPGLIGVSTSCVVGYFIALSRGLV"
     gene            704..1471
                     /locus_tag="AC16G0_17030"
     CDS             704..1471
                     /locus_tag="AC16G0_17030"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5101360.1"
                     /translation="MRLHAHANRRLPRWFFVCALCLALVGPEALAHSPLVAEVNAFSS
                     RYHERLARIDTMRDELRQVTRNGADAQELIAFSQVCFIWGDIRATTIKQKLEAYDEGR
                     QAAKRAVEMDPRNPEAHFWYGTNTARWGATEGVVRSLFLLPSVQQEIQMVLDLDPNFT
                     PVYALAGNVLYEVPPLMGGDLRKAEEMFRKGLEQDPRFTGMRIGLAKTLIKLGRTAEA
                     RKELQAVLNEKNPRNMGEWVMKDTKEARQLLQSLDSN"
     gene            1719..1958
                     /locus_tag="AC16G0_17035"
     CDS             1719..1958
                     /locus_tag="AC16G0_17035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101361.1"
                     /translation="MDAIWAVFHEPGSHRYAVHFLDPGTRDLENRVVVGKDFHGGLEL
                     ARAALKGQGMGDGLFLDRTLRRPLSTPVLSDLSAL"
     gene            2308..>2945
                     /locus_tag="AC16G0_17040"
     CDS             2308..>2945
                     /locus_tag="AC16G0_17040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101362.1"
                     /translation="MVGILSLLLLVFFAAALTASQGLPPNLLAVVPGDQRPEVENILA
                     NPTFVRGLDFAASADLQVYTYLLEHPDLNAALAQALGIAHYEVVRLGPGHYRGTDGNG
                     NVGTIDVFRDEGHERAFLEQGVSSGWWFGDIGGRVVALILFAPEAEGVRGTVTVWARI
                     DQGMVDHLLRALRPMLGGFLDRKLREQFDIPTRVAETAAQHPDQFCPHLWAVS"
BASE COUNT          502 a          915 c          964 g          564 t
ORIGIN      
        1 atcggaaagt acgtgatcaa ccacagcttc atcctgcctg gtttgatcgg cgtgtcgaca
       61 tcgtgcgtgg tgggctactt catcgcgctg agcagggggc tggtctagtc ttcaagccct
      121 tcgatgaccc gtggggagat cttcgtgagg ccgcttgtaa tccgggaccc ctgtttcctc
      181 aatcactcga gggtttccgt cttggtgggc tatggtccag aggccgagtt cggtgcgcgc
      241 agaataccgc ctacatgcag gatattgcgt tgcaggagga attccactcg acgccgagtc
      301 agacctgcct gcccctgcgt ctagtgctca tgaatcttcc cggcgggtcg tcacctcccg
      361 gctcttcgaa atgcttgagg gcactcagcg aggacgccgg caggggcgcg accgcagcaa
      421 atcgtgcggt ggacgagtgg ctccggcaag ctatcgctgg ggtgcatgag cgccgcgtga
      481 ccggcgagat gatcgattcc tgtcccgacc agggcactga ggtgccgctc tcaggagggg
      541 gcatcaatcc atgagatcag gcagccgacg catccgaagc cgctgaggtg agtcggtcca
      601 gcagtggccc gtggatgggt tgattcttat cccgcgacgg cgcggggatt tgtgtgagag
      661 caacgggcga caagacgccc ggacgataga gggaaagcga tccgtgagac tgcatgcgca
      721 cgcaaaccgc cgattgcccc gctggttctt cgtctgcgcg ctctgcctcg ccttggtcgg
      781 accggaggcg ttggcccact cgccgctggt agccgaagtc aatgccttct cctcgcggta
      841 ccatgagcgc ctggcccgga tcgataccat gcgggacgag ttgcgacagg tgacccggaa
      901 cggagccgat gcgcaggagc tgatcgcctt ctcccaggtc tgcttcatct ggggcgacat
      961 tcgcgccacc acgatcaagc agaagctcga ggcgtacgac gagggccgcc aggccgcgaa
     1021 gcgggcggtc gagatggatc cgcgtaatcc ggaggcgcat ttctggtacg gcacgaatac
     1081 cgcccgctgg ggagccacag agggtgtcgt gcgatccctc tttctcctcc cctccgtgca
     1141 gcaggagatc cagatggtgc tggatctcga cccgaacttc acccccgtct atgcgctggc
     1201 cggcaacgtg ttgtacgagg tgccgccgct catgggaggc gacctgcgca aggcggagga
     1261 gatgtttcgc aaggggctcg agcaggatcc ccgcttcacc gggatgcgca ttggcttggc
     1321 aaagacgctg atcaagctgg gccggaccgc cgaggcgcgg aaagagcttc aggccgtgct
     1381 gaatgaaaag aatccccgca acatgggcga gtgggtcatg aaggacacga aggaagcccg
     1441 ccagctcctg cagagtctcg acagcaactg aggcgcgggc tccttccccg gatctgggcg
     1501 tgtgatgccg ctcggtgtgg cctctctgcc ggtcctgaac gccctcctca acgctgtcag
     1561 cgcgctgctg ctcgccggcg gacatgcctt cgtccgacgg atcgaggcgc acatgctcgg
     1621 cgcgtttggc gtatccgtcg gatacctccc gcttcgctac ttcgctggca tgacccgctt
     1681 cgcgggtcaa ggggtgggtg cgccaagccc attttacgat ggatgcgatt tgggcggtat
     1741 tccacgaacc gggctcccac cgctatgcgg tccacttcct cgatcccggc acgcgagacc
     1801 tcgaaaacag ggtggtggtc ggaaaggatt tccatggagg gctggagctc gccagggccg
     1861 cgctcaaggg acagggtatg ggagatggtc ttttcctcga ccggacgctt cgccgcccgc
     1921 tctcgacgcc agttctctcg gatctgtcag cgctgtgacg actcgattca gattgaagcg
     1981 tagatcccgc ctcctgccgc ttcgcaatcc ggacgtcgcg ggtcaccatt ccctctaagg
     2041 tcaagcctcg caccgctcga tgatcagaag gggccccgcg agtcacggca aggaatcagg
     2101 ccgggccttg ctgagattcc gcacgcacac gtgcagaacc gcacccgacg tgcaaagact
     2161 gtctgtgcga ggcgttcctt gtgccgcctt tcctgcggac ttgcccgttc tcccgttggc
     2221 tcggtttatg ctctctagga ggccaggcag cgaggaaacg atgtccagca aggttgcggg
     2281 aagggaacgg cgctgatcat gcggtcgatg gtaggaatcc tcagcctgct tcttctggtg
     2341 ttcttcgcgg cggcgctcac tgcgagccag ggtctgccac cgaacctcct ggccgtggtc
     2401 ccgggggatc agcgccccga ggtcgagaac atcctcgcca acccgacctt cgtgcggggg
     2461 ctggattttg cagcgtccgc ggatctgcag gtttacacct acctgctcga gcaccccgac
     2521 ctgaatgcgg ccctggccca ggccctgggc atcgcgcact acgaggtcgt ccgcctcggc
     2581 cccgggcact atcgcgggac ggatggtaac ggcaatgtgg gcaccatcga cgtcttccgt
     2641 gacgagggac acgagcgggc cttcctggag caaggggtct cttccggctg gtggttcggc
     2701 gacatcgggg gccgcgtggt ggccctcata ttgttcgcgc ccgaggcgga gggcgtgcgg
     2761 gggaccgtca cagtgtgggc ccgtattgac caggggatgg ttgatcacct cctacgcgcg
     2821 ttacggccga tgctgggggg gtttctggac cgaaaactcc gcgagcagtt cgacatcccg
     2881 actcgggtag ccgagactgc tgcacagcac cccgaccagt tctgccccca cctgtgggca
     2941 gtttc
//
LOCUS       DBNGZC010000990         2225 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_966914, whole genome shotgun sequence.
ACCESSION   DBNGZC010000990 DBNGZC010000000
VERSION     DBNGZC010000990.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_966914"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="AC16G0_17045"
     CDS             complement(<1..896)
                     /locus_tag="AC16G0_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5101363.1"
                     /translation="MHESPLLEVEDLRVEFPTPGGSVRAADGVSFRVWRGEIVGLVGE
                     SGCGKSVTTLALLRLVRPPGRIASGRIMFKGRDLLALPEHEMRLLRGGEMAMIFQNPM
                     TALDPVFTVGDHLTEVFRAHPQLHTGDLQTAAAALLERVRIPNPRAQVKAYPHQFSGG
                     MAQRVMIGMGLAGRPSLLIADEPTTALDVSVQAQILDLLRAIRTELQTAIILLTHDLA
                     ITAQLCDRLVVMYAGQVLEASRTRDLFRSPQHPYTRGLLDSMPRLGHMHPRLEQIPGQ
                     PPDLRALPVGCRFAPRCPRAEER"
     gene            complement(898..1803)
                     /locus_tag="AC16G0_17050"
     CDS             complement(898..1803)
                     /locus_tag="AC16G0_17050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5101364.1"
                     /translation="MTAHAASPQAPSAVRELAGRRLPRFQSGDVLVLAAFLTLAIIAL
                     CALIPSTLAPHPPNRQEITMRLRPPGWMARGNPAYPLGTDQLGRDLLSRVIHGARISL
                     IVALSALAISGTVGVSIGLLAGYYRGWLDDWIMRLADVQLAIPYVLLAIAIIAVIGPS
                     NRNVVLVLAVNGWVVYARLARGQALSLREREFILAYRALGARDWRIVLQHILPNLLSA
                     VIVVASLELAGIITLESSLSFLGLGIQPPTVSWGYMLADGRTYLLGGAWWVVTFPGLA
                     ITAAVLSINVLADWLRDVLDPRLKI"
     gene            complement(1800..>2225)
                     /locus_tag="AC16G0_17055"
     CDS             complement(1800..>2225)
                     /locus_tag="AC16G0_17055"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5101365.1"
                     /translation="GAGGIRYLILPAATLALYPMARITRLVRSSLVDVLNQDYILAAR
                     ARGLWEALVVGKHALKNAALPVVTIVALQFGYMLGGAVITETIYGWPGIGHYTVTAIY
                     ERDFPVVQAAVVLASGMFIGLNLLVDLLYGWLDPRIRYT"
BASE COUNT          378 a          748 c          791 g          308 t
ORIGIN      
        1 cgctcctcgg cccgcgggca gcggggagcg aagcgacagc ccacggggag ggctcgcagg
       61 tccgggggct ggccggggat ctgctccagc cgggggtgca tgtgccccag gcgcggcatg
      121 gagtcaagga ggccccgggt gtaggggtgc tgcggggaac gaaacaggtc gcgggtgcgg
      181 ctcgcctcca gcacctgtcc cgcgtacatc accacgagcc ggtcgcagag ctgggcggtg
      241 atggccaggt cgtgggtgag gaggatgatg gcggtctgta gctccgtccg gatcgcgcgc
      301 aggaggtcca gtatctgggc ctgcacgctc acatccaggg ccgtggtggg ttcgtccgcg
      361 atcagcaggg acggccgccc ggccaggccc atgccgatca tcacccgctg ggccatgcca
      421 ccgctgaact ggtgtgggta ggccttcacc tgcgctctcg gattggggat gcgaacccgc
      481 tcgaggagcg cggctgccgc ggtctgcagg tcgcccgtgt gaagctgtgg atgggcccgg
      541 aacacctcgg tcaggtggtc gcccaccgtg aagacggggt cgagagccgt catggggttc
      601 tggaagatca tggccatctc cccgccccgg agtaatcgca tctcgtgttc ggggagggcc
      661 aggaggtccc tccccttgaa catgattcgc ccggaggcga tccgccccgg cggtcgcacc
      721 agacgcagca gggccagggt ggtgaccgat ttcccgcagc cgctctcgcc cacgaggccc
      781 acgatctcgc cccgccagac acggaaggac acgccgtcgg ccgcgcgcac cgaccccccg
      841 ggcgtcggaa actccacccg aaggtcctcc acctctagga gcggcgactc atgcatctca
      901 gatcttcaag cgggggtcca gcacatcgcg cagccagtcg gccaggacgt taatgctcag
      961 gaccgctgcg gtgatcgcca gtccgggaaa ggtgaccacc caccacgctc cgcccagcag
     1021 gtaggttcgc ccgtccgcca gcatgtaacc ccagctcacg gtgggcggct gaatccccag
     1081 gcccaggaac gagagcgagg actccagggt gatgatcccc gccagctcca gcgatgccac
     1141 cacgatcacg gcgctcagca ggttgggcag gatgtgctgc aggacgatac gccagtctcg
     1201 ggcgcccagg gcgcggtagg ccaggatgaa ctcccgctcg cgcagggaga gggcctggcc
     1261 gcgggccaga cgggcataca cgacccagcc gttcaccgcc aggaccagca ccacgttgcg
     1321 gttgctgggg ccgatcaccg cgatgatggc gatggcaagc aagacgtagg ggatggcgag
     1381 ctgcacgtcc gccagccgca tgatccagtc gtccagccag ccccggtagt atcccgccag
     1441 gagcccgatg gacacgccca ccgtccccga gatcgccagc gcgctcagcg ccacgatgag
     1501 cgagatgcgc gcgccatgaa tcacccgcga gaggaggtcc cgcccaagct ggtcggtccc
     1561 cagcgggtag gcgggattcc cgcgcgccat ccagccgggc ggccggagcc gcatcgtgat
     1621 ctcctgccgg ttggggggat ggggggcgag ggtcgagggt atcaaggcgc agagggcgat
     1681 gatggccagg gtcaggaagg cggcgagcac caagacgtcc ccgctctgga agcggggcag
     1741 ccgtcggccg gcgagctcgc gcaccgcgct cggcgcctgg ggcgatgcgg cgtgggcggt
     1801 catgtgtagc ggatcctggg gtccagccag ccgtacaaga ggtccaccag gaggttgagc
     1861 ccgatgaaca tgccggaggc caggaccacg gcggcctgca ctaccggaaa gtcgcgctcg
     1921 tagatcgccg tcacggtata gtgaccgatc cccggccagc catagatcgt ttcggtgatc
     1981 acggcgccgc ccagcatgta cccgaactgt agcgccacga tcgtgaccac tggcagggct
     2041 gcgttcttca gcgcgtgctt gcccaccacc agcgcttccc acaggccgcg ggcgcgcgct
     2101 gccaggatgt agtcctggtt cagcacgtcc accaggctgg agcggaccag gcgggtgatg
     2161 cgcgccatgg ggtacagcgc cagcgtggcc gcgggcagga tcagatacct gatccctccc
     2221 gcgcc
//
LOCUS       DBNGZC010000991         1577 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_280309, whole genome shotgun sequence.
ACCESSION   DBNGZC010000991 DBNGZC010000000
VERSION     DBNGZC010000991.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_280309"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="AC16G0_17060"
     CDS             <1..1019
                     /locus_tag="AC16G0_17060"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013416163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HIR5101366.1"
                     /translation="EATLCVLDAVQQKDSRLRFEWVPLAMGWEAIRKNGSAIPESTVE
                     ALEKCDAWIMGPHDSASYPAAEREKLNPSGTLRHRFDLYANIRPARAYPGLHAVCPKM
                     DLVIARENTEGFYADRNMAQGPGEFMPTPDVALAVGKITRQASERIARTAFALARRRR
                     RKLTIVHKANVLRTTFGLFMDVCRGVGAEFPDVAVDDYHIDAMTAHLVRRGADFDVIL
                     TENMFGDILSDLAGELSGSLGMAPSINAGTSRAMAQAAHGSAPDIAGRGIGNPVGIIL
                     SAAMLLTWLSGEHGDAAAAEAGRAIEQAVAKTLEVGPRTRDLGGTAGTKEFTSAILGH
                     LNGS"
     gene            1095..1571
                     /locus_tag="AC16G0_17065"
     CDS             1095..1571
                     /locus_tag="AC16G0_17065"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR5101367.1"
                     /translation="MQKAEWIVAALCVLLGLGILWQAWGMEYLTSIGPGPGFFPRWLG
                     LMLTGLSAAWLLSAVWRSGRKEDRRFFPEWQGLRRILLVVGAIIAVGVAMEFVGFQLA
                     MFAFVVFVLTALGWRGWGLTVVLSAVMSFGVYHAFTRWLDVTLPQASIEVLRRWGF"
BASE COUNT          257 a          493 c          539 g          288 t
ORIGIN      
        1 tcgaggccac gctttgtgtg ctggatgcgg tgcagcagaa ggacagtcgc ctccgatttg
       61 aatgggtgcc gctggccatg ggctgggagg cgatccggaa gaacggaagc gccatcccgg
      121 agtccaccgt ggaggcactc gagaagtgcg acgcctggat catgggtccc cacgactcgg
      181 cctcctaccc ggcggccgag cgggaaaagc tgaaccccag cggcacgctg cggcatcgct
      241 tcgatctcta tgcgaacatc cggccggctc gcgcgtaccc cggcttgcac gccgtttgtc
      301 ccaagatgga cctggtgatc gcgcgagaaa acaccgaggg attctacgcc gatcggaaca
      361 tggcccaggg acccggcgaa ttcatgccga ccccggacgt ggcgctggct gttggcaaga
      421 tcacccgcca ggcctcggag cggatcgccc ggacggcatt tgcgctggcg cggcgacggc
      481 gacggaaact tacgatcgtg cacaaggcca atgtgctccg cacgaccttc gggctgttca
      541 tggacgtctg ccgtggagtc ggcgccgagt ttcccgacgt ggctgtcgac gactaccaca
      601 tcgatgccat gacagcccac cttgtccgcc gcggggccga tttcgacgtc atcctcacgg
      661 agaatatgtt cggcgacatc ctgtcggatc ttgcgggaga gctgagcggg agcctgggca
      721 tggccccctc gatcaatgcg ggaaccagcc gggccatggc gcaggcagcc cacggttcgg
      781 ctccggatat cgcaggtcga ggtatcggga acccagtggg gatcattctc tcggcggcga
      841 tgctcctcac gtggctctcc ggggagcacg gggacgccgc cgcggcagag gccggccgcg
      901 ctatcgagca ggccgtggcc aagacccttg aggtcggacc caggacccga gacctgggcg
      961 ggaccgccgg caccaaggaa ttcacctcgg ccatccttgg acacctcaac ggttcgtagc
     1021 agacacacca gccaagctga cccaagcggt cgttgcggga agcgccgaag gcatctggac
     1081 aaggagtcga gttgatgcag aaagcggagt ggatcgtcgc ggccctgtgc gtcctcttgg
     1141 gccttggaat cctttggcag gcctggggga tggagtacct cacctccatc ggccccgggc
     1201 ctgggttctt cccccggtgg ctggggctca tgctcacggg gttgagtgcc gcctggctgc
     1261 tgtcagccgt gtggcgctcc ggacgaaagg aggatcgtcg tttcttccct gagtggcaag
     1321 gtcttcggcg catcctgctt gtcgtggggg cgatcatcgc cgtgggcgtc gcgatggagt
     1381 tcgtcggctt tcaactcgcc atgttcgcct tcgtcgtctt cgtgctcacg gccctgggct
     1441 ggcggggatg gggactcacg gtcgttctct ccgccgtgat gagcttcggg gtgtatcacg
     1501 ccttcacgcg ctggctggac gtgaccctcc cgcaggcgtc catcgaggtc ctgaggcgct
     1561 ggggttttta gggagag
//
LOCUS       DBNGZC010000992         2163 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_671121, whole genome shotgun sequence.
ACCESSION   DBNGZC010000992 DBNGZC010000000
VERSION     DBNGZC010000992.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_671121"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..2011)
                     /locus_tag="AC16G0_17070"
     CDS             complement(56..2011)
                     /locus_tag="AC16G0_17070"
                     /inference="COORDINATES: protein motif:HMM:NF014073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR5101368.1"
                     /translation="MWDSAVVALGMFLDPFHLLMLLVGVVEGIVIGLLPGMGGTACVA
                     ILLPFIYQLGPHTAMAVLVGALAVVHTSDTITAVLIGTPASSASATTVMDGHPMARQG
                     QAARALSAAFLSSLIGGFIGVLALTLSIPVARPLVLSFGSPELFMLCFLGLGFAAFLT
                     GGTPLKGLVSAALGLLVGAVGVAPNVAHYRYTFDQLYLMDGLPLVCVALGLFGVAEVV
                     DLLAAGGKIAERMDLGHGWMQGVRDVIQHRWMLVRGSLIGVWTGILPGIGASAGSWMS
                     YGHAVVVSKDREQFGKGDVRGIIAPEAANNAVEAGDLIHTLLFSVPGGAPSAILMGAL
                     IVFGIQPGPRMITEHMDLLFTVIWSFALANIIGAALCLALVIPLARLTAIRFDYLAPA
                     ILIIMIFGAYQSTRHMGDLSVMLGLGLLGWGMKRLGWARPPFLLAFVLSNPTERYLWI
                     SVNRYGLSWLERPGSLAILALLLASLVYWIVSQKRKKLGDAVQVRPEWRFRPKAVFTL
                     GLMLLLAAAAWDASGFPFYAGLLPMVVAIPGVAMAAWQFVLDLRGGDEKKEVLDLATS
                     EAAASRKGTLRALLVFGIFVAYYCLILAVGFRIATLVFLVGFLRFLPRAGWRRTAIYT
                     ACMFAVIEAFTRLLGMELPEGIWQLWP"
BASE COUNT          457 a          720 c          696 g          290 t
ORIGIN      
        1 cgccgacgtg cgggtaatcg cgtgtggcgc tcgttcacag acgagtggga ggtgatcagg
       61 gccatagttg ccagatccct tccggcaatt ccatgcccag cagccgggtg aacgcctcga
      121 tcacggcaaa catacaggcc gtgtagatcg cggtgcgcct ccagcccgca cgcggcaaga
      181 accgtaggaa gcccaccagg aataccaggg tcgcaatccg gaacccaacc gcgaggatga
      241 ggcagtaata cgccacgaag atcccgaaga ccagcagggc gcgcaatgtc cctttgcggg
      301 aggccgcggc ctcggaggtg gccagatcca gcacctcctt cttctcatcc ccgcccctga
      361 gatccaggac gaactgccag gccgccatag cgactccggg gatggcgacc accatgggga
      421 ggagacccgc atagaatgga aaccctgagg cgtcccaagc cgcagccgcc agaagaagca
      481 tgagccccag cgtgaagacg gccttcggcc ggaagcgcca ttccggcctc acctgcacgg
      541 catcgcccag tttcttccgc ttctggctca caatccaata caccaggctg gcgagcagga
      601 gcgcgaggat ggccaggctc cccggacgtt ccagccagct cagcccatac cgattgacgg
      661 agatccagag gtatcgttcg gtgggattgc tcaggacgaa ggcgagaagg aagggtgggc
      721 gcgcccagcc gaggcgcttc attccccacc ccagcaggcc gagccccagc atcaccgaga
      781 gatcacccat gtgacgcgta ctctgatacg cgccgaagat catgatgatg aggatcgccg
      841 gagccaggta atcaaagcgg atggccgtca ggcgggccag cggtatgacc agcgccaggc
      901 acagggccgc gccgatgatg ttggccaggg cgaagctcca gatgacggtg aagagcaggt
      961 ccatgtgctc cgtgatcatc cggggccctg gctggattcc gaagacgatg agggctccca
     1021 tcaggatggc cgagggtgcc cctcccggaa cgctgaacag gagcgtgtga atgagatccc
     1081 cggcctccac tgcgttgttg gcggcctcgg gcgcgatgat cccgcggaca tcgcctttcc
     1141 cgaactgttc ccggtccttg gagacgacca cagcatgtcc atagctcatc caggagcccg
     1201 cgctggcacc gatgcccggc aggatccccg tccagacgcc gatcagggac ccccgcacga
     1261 gcatccagcg gtgctggatc acatcccgga cgccctgcat ccatccgtgg ccgagatcca
     1321 tccgctcggc gatcttgccg ccagcggcca aaagatccac gacctcggcc accccgaaca
     1381 gccccagggc cacacagacc aggggtaggc catccatgag gtagagctgg tcgaaggtgt
     1441 agcggtagtg ggccacgttg ggggcgacac cgactgcccc cacgaggagg cccagggctg
     1501 cagagaccag acccttcagg ggagtcccgc ccgtgagaaa ggcggcgaag ccgagaccga
     1561 ggaagcagag catgaacagc tccggcgagc cgaagctgag gaccaacggt cgggccacgg
     1621 gaatcgagag cgtgagggcc agaaccccga tgaatccccc gatcagggag gagaggaagg
     1681 ccgcggacag cgcccgggcc gcctggccct gccgcgccat cgggtgacca tccatcacgg
     1741 tggtggccga ggccgacgag gccggcgtcc cgatgagcac agcggtgatg gtgtccgagg
     1801 tatgcacgac cgccagtgcg cccaccagca cggccatggc ggtgtggggg cccaactggt
     1861 agatgaaggg aagcaggatg gccacgcagg cggtcccgcc catccccgga aggagcccga
     1921 tcacgatccc ctcgaccacg cccaccagga gcatgagcag gtggaacgga tcgaggaaca
     1981 ttcccagggc cacaacggcg ctatcccaca tgcacactcc ctcggccccg gccgcaaacc
     2041 gcgagcccaa ggccgcgtga cctatccgcg atgaatcttc cgcccgatct atttcaccac
     2101 gacgctgtac ttcttttcca ggaggtccag gaggtagcgc cgggcctccg gcgggagctg
     2161 gaa
//
LOCUS       DBNGZC010000993         2599 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1261076, whole genome shotgun sequence.
ACCESSION   DBNGZC010000993 DBNGZC010000000
VERSION     DBNGZC010000993.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1261076"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="AC16G0_17075"
     CDS             complement(<1..1044)
                     /locus_tag="AC16G0_17075"
                     /inference="COORDINATES: protein motif:HMM:NF013974.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR5101369.1"
                     /translation="MTRRVLMGNEAIGRGIVECGCAVAASYPGTPASEILEAVVTSAR
                     ETGQRIHAEWSANEKVAYEVALAASYTGKRAAVAMKQVGLNVASDPFMRSAYLGVKGG
                     LVIICADDPGPHSSQTEQDSRFYAMFAKIPVFDPSSPREAKEMVGPAFDLSEAYEIPV
                     MLRPTTRVCHARQAVPLQEPVREGRPARFQKDPRRWCATPRFVLDLHRGLSEKLARLA
                     ADPRLAPVLLAGDGSFPRTCLIASGAAFAHAWDVLEEVGLFGKVDLYQVKVPFPLAAD
                     FISTVVPAYERVLVIEETYPVIEMQLADRRIEGRRSKLVPNEGELTPEVIRDVLERFL
                     GLAPTPQTSAPQAG"
     gene            complement(1041..1919)
                     /locus_tag="AC16G0_17080"
     CDS             complement(1041..1919)
                     /locus_tag="AC16G0_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5101370.1"
                     /translation="MGSLQDLVQFGFAGLTSGAIYAMVAIGFGVVHNTLGIVNFTQVD
                     FVSLGGMLLYAALLGAKLPAIVALPVAVAGVTLAGALAEFLGLRPSRSRNHLVLILLT
                     IGMSIALRGVMKQVWGKNSLAVPPLTGETPITVLGATMVPQAVWILLITALAIALLVL
                     FFRKTTTGLAMRAVASNPTAAAVVGLKVWRVRTLSFALAGALGGLAGVLVTPITTLRY
                     DVGVLLGLKGFSAAILGGFGSFPGAILGGLSLGLLESLGAAYVSSAWKDSIAFLVLLL
                     VLFVRPTGLTGKAEAP"
     gene            complement(2201..>2599)
                     /locus_tag="AC16G0_17085"
     CDS             complement(2201..>2599)
                     /locus_tag="AC16G0_17085"
                     /inference="COORDINATES: protein motif:HMM:NF020419.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HIR5101371.1"
                     /translation="AYVEPVVSLDLDLVVAVDQLLQVETLLASAFRLERHAHSLNVSQ
                     SGSDLRIQIQTDPRYIPFVERAVPREVLGISLPVASIEDVMQGKVWAATDPSRRPSKR
                     LKDLADIARLLEVAPHLRPLVPAEVLAKLV"
BASE COUNT          497 a          825 c          891 g          386 t
ORIGIN      
        1 gccggcctgc ggcgcagacg tctgcggtgt cggggcgagt ccgaggaaac gttcgagaac
       61 gtcgcggatg acctcgggcg tgagctcccc ttcattcggg acgagcttcg agcgcctgcc
      121 ctcgatccgc cggtctgcga gctgcatctc gatcaccggg tacgtttcct cgatgaccag
      181 gacgcgctcg taggcgggga ccaccgtgct gatgaagtcc gcggccaggg ggaagggcac
      241 cttcacctga tagaggtcca ccttcccgaa gagccccacc tcctccaaga catcccaggc
      301 gtgggcgaag gccgcccccg aggcgatgag gcaggtgcgc gggaacgagc cgtctcccgc
      361 caggagcaca ggggcgagac gcgggtcggc ggccaggcgc gcgagcttct cgctgagtcc
      421 gcggtgcagg tccaagacga agcggggcgt ggcgcaccag cgcctgggat ccttctggaa
      481 tcgggccggt cgcccctccc ggacaggctc ctggagcggg acggcctgcc gggcgtggca
      541 gacccgcgtc gtcggccgga gcatcaccgg gatctcgtac gcttccgaca agtcgaaggc
      601 cggcccgacc atctccttgg cctcgcgggg gctcgagggg tcgaagaccg gaatcttggc
      661 gaacatggcg tagaaccggc tgtcctgctc ggtctgggag ctgtggggcc cggggtcgtc
      721 ggcgcagatg attaccaggc cgcctttgac cccgagatag gcggagcgca tgaagggatc
      781 cgaggcgaca ttcaacccca cctgcttcat ggccacggcg gcgcgcttcc ccgtgtagct
      841 cgcggccagg gccacctcgt aggccacctt ctcgttggcc gaccactcgg cgtggatccg
      901 ctgtcccgtc tcccttgcgg aggtcacgac cgcttccagg atctccgagg cgggcgtgcc
      961 ggggtaggaa gccgccacgg cgcagccgca ctccacgatc ccccgaccga tggcctcgtt
     1021 ccccatgagc accctgcgcg tcacggtgct tctgcctttc ccgtgaggcc tgtcggtctc
     1081 acgaagagga ccaggaggag gacgaggaag gcgatgctgt ccttccaggc gctgctcacg
     1141 taggccgcgc ccaaagactc caggaggccc agggagaggc cgcccaggat ggcgccgggg
     1201 aacgagccga acccgcccag gatggccgcg gagaaaccct tgaggccgag gaggacgccc
     1261 acgtcgtagc gaagcgtcgt gatgggcgtg accaggacac cggccagacc tcccagggcg
     1321 ccggccagag cgaagctcag cgtcctgacc cgccagacct tgaggccgac gacggccgcg
     1381 gcggtgggat tggaggccac ggctcgcatg gcgagccccg tagtggtctt cctgaaaaag
     1441 aggactaaca gggctatggc cagggccgtg atcaggagga tccagacggc ctggggcacc
     1501 atggtcgctc ccagaacggt gatcggcgtc tcccccgtga ggggtggcac ggccagggaa
     1561 tttttccccc agacctgttt catgacgccg cggagagcaa tggacattcc gatggtcagc
     1621 aggatgagga cgaggtggtt ccgggagcgc gagggtcgca gccccaggaa ctccgccaac
     1681 gccccggcga gggtgacgcc cgccacggcc acgggcaggg ccacgatcgc gggaagcttg
     1741 gcgccgagga gcgcggcgta gaggagcatc ccgcccagag acacgaagtc cacctgggtg
     1801 aagttgacga tgcccaaggt gttgtggacc acgccgaaac cgatggccac catggcatag
     1861 atggccccgc tggtcagacc cgcgaacccg aactggacaa ggtcttgaag cgaacccatc
     1921 gccggcggga ccctcccttg atcctgaacc tcaaacgagg cagaaggaaa ccgatacata
     1981 tatgaatcga tccggtcaag gcaaggagaa tgaaagtttc cgggaacttg ggaccaaaga
     2041 gcgcaccttt cctctcggcc agattagggc agttcaaccg ccgctactac cggagtaacc
     2101 gttggtgcgc aaagatattt cccaaattcg aaccgccgac gggaaatcag ccggatgaat
     2161 agctgggaag ttttcgatcc tcaggttggc tacgcggcga tcagaccagc ttcgcgagga
     2221 cctcggcagg gacgaggggg cgcagatgcg gggcgacttc gaggaggcgg gcaatgtcgg
     2281 ccaggtcctt gaggcgcttg ctgggccggc gggaggggtc cgtggccgcc caaaccttcc
     2341 cctgcatgac gtcctcgatc gaggccacgg gcaaactgat tcccaggact tcgcgcggga
     2401 cggcacgctc cacgaacggg atgtatcgag gatcggtctg gatctggatg cggagatcgg
     2461 accccgactg tgagacattc aggctgtgcg cgtgccgttc aagtcgaaat gcagaagcaa
     2521 ggagagtctc cacctgaaga agctggtcca ccgccacgac cagatctagg tcgagactga
     2581 ccaccggctc aacgtaggc
//
LOCUS       DBNGZC010000994         3384 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2039894, whole genome shotgun sequence.
ACCESSION   DBNGZC010000994 DBNGZC010000000
VERSION     DBNGZC010000994.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2039894"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="AC16G0_17090"
     CDS             <1..353
                     /locus_tag="AC16G0_17090"
                     /inference="COORDINATES: protein motif:HMM:NF025559.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HIR5101372.1"
                     /translation="VEIARKQGKALASQLGGNSLQDFASGKEPWVRGDALRVEVLQET
                     KSAYDFNVTRCRYAEMYRTLGIPELGAVLSCGRDYALGEGFNPNLKLARTQTIMEGAP
                     FCDFRYRVEPPGDQ"
     gene            467..880
                     /locus_tag="AC16G0_17095"
     CDS             467..880
                     /locus_tag="AC16G0_17095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR5101373.1"
                     /translation="MKFHTQYLTFKTRRHREYVNITREVEAALKTSGIREGMILVSAM
                     HITAAVYVNDAEDGLIQDIDEWLEGVAPTRPDYRHHRTGESNGDAHLKNLLMHHQVLI
                     PVTQAKLDFGPWQQVYYAEFDGQRPQRVIIKVMGE"
     gene            896..1081
                     /locus_tag="AC16G0_17100"
     CDS             896..1081
                     /locus_tag="AC16G0_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101374.1"
                     /translation="MDPAEDPAFRALVEQAKRVCAKNPTLLPPYLAAIREKGRGLPEE
                     ALRAILDEVKAATLISS"
     gene            1337..2005
                     /locus_tag="AC16G0_17105"
     CDS             1337..2005
                     /locus_tag="AC16G0_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR5101375.1"
                     /translation="MLSEAVQDYLKAIYKLQQTGGIVTTSALAEAMGVAAPSATGMVK
                     RLAGLKLVRHHPYRGVLLTKAGQKMALEVLRHHRLLELYLAEALGYTWDKVHEEAERL
                     EHVISEELEEKIFQALGRPTRDPHGEPIPGRDGTLAAVEIEPLSELAAGTAGVIRQVS
                     DSHAEMLRYLGARGLVPDAAVEILDRAPFNGPITVRIGKVSHMLGRELAGHIWVQIST
                     NGVS"
     gene            2053..>3384
                     /locus_tag="AC16G0_17110"
     CDS             2053..>3384
                     /locus_tag="AC16G0_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HIR5101376.1"
                     /translation="MADNDALTEGQTSSPESSTSVLSGDERTLAAAAEVLEGRSHEAW
                     HRRLLPFLGPAFIASVAYVDPGNFATNIQGGAQFGYLLLWVIVASNLMAMLVQALSAK
                     LGIATGRNLAEMCRDQFPRWVVWTMWGLMEIVAMATDLAEFLGAALGFNLLFGMPLFV
                     AGLLTGVATFAILALERHGFRPLEAIITSLVGVIAFCYLIETFLGRPDWGQIVYHSLV
                     PTFSGAESVILATGILGATVMPHVVFLHSALTQGRIVTRKPEQLRRLFRYECVDVTIA
                     MGVAGLVNGAMLVMAAATFFQKGLTTVGTIEQAHQTLVPLLGRASSWVFAISLLASGL
                     SSSTVGTMSGQIIMQGFVHFGIPIWLRRLLTMLPALVVIAVGMEPTRTLVLSQVVLSF
                     GLPFALVPLVLFTARSDLMGVLVNRRLTTLLASLVTLLIIALNLFLLYQIIW"
BASE COUNT          595 a         1025 c         1123 g          641 t
ORIGIN      
        1 tcgtggagat cgcccgcaag caggggaagg cactggcaag tcaactcgga ggaaacagcc
       61 tgcaggactt cgcgagcggc aaagagccat gggtgagggg ggacgccctc cgggtcgagg
      121 tcctgcaaga gaccaaatcg gcgtacgact tcaacgtcac ccgatgccgg tacgcggaga
      181 tgtaccggac gctgggcatc ccggagcttg gcgctgtgct gtcctgcggg cgggactacg
      241 ccctgggcga aggcttcaac ccgaatctga agctcgcgcg aacccagacg atcatggagg
      301 gggcgccctt ttgcgacttc cggtaccggg tggagccccc aggggatcag tagggaccgt
      361 ggacgtcgca ggttcttttc cagaaaaccg tttcggaccg tctgcggaat ctgcaagatc
      421 tctgatgaat tcagaagttc ttccggcggt ctcctggaga accggcatga agttccacac
      481 ccagtacctg accttcaaga cgaggcgcca ccgggaatat gtcaacatca cccgggaggt
      541 ggaagcagcc ctcaagacga gtggcattcg cgaggggatg attctggtct ccgccatgca
      601 catcacggct gccgtgtatg tgaatgacgc cgaggacggt ctcatccagg acatcgacga
      661 gtggctggag ggggtggcac ccaccaggcc agactaccgt caccacagga cgggcgagag
      721 caacggcgac gcccacctga aaaatctgct catgcaccat caggtcctca tcccggtcac
      781 gcaggccaag ctggacttcg ggccgtggca gcaggtgtac tatgcggaat tcgatgggca
      841 gcggccacag cgcgtgatca tcaaggtcat gggcgagtag cggcaggagg gagagatgga
      901 ccctgcagag gatccggcct tccgggccct ggtggaacag gccaagcgag tgtgcgcgaa
      961 gaatccgact ctcctgccac cctatctcgc cgccatccga gagaagggga ggggtctccc
     1021 agaggaggcg ctccgagcca tcctggacga ggtgaaggca gcgacgctga tatcctcctg
     1081 aggccagcgg gagcggcggg accctctgcc accgcgcaca cgtctgggaa gcttgccctc
     1141 tggccgtgcc gggacgggcg gtgcccgcct gtccactgcg catccccagc cacccctgca
     1201 atgttgacca tccctagcct gccacggtag cccgaccggt ttcgagacat ccggcttccc
     1261 caccgacttc gtgcttgacg ggggatggct gggcggatac atttagccaa gactaaagat
     1321 attcttcggg ggctgcatgc tcagcgaggc ggtgcaggat tatctcaagg ccatctacaa
     1381 gctccagcag accgggggga ttgtaaccac ctcggccctg gcggaggcca tgggggtggc
     1441 cgccccctcg gccacgggca tggtgaagcg gctggccggg ctgaagctgg tgcgccacca
     1501 cccgtaccgg ggggtccttc tgaccaaggc cgggcaaaag atggcgcttg aggtgctccg
     1561 gcaccaccgg ctcttggagt tgtatctggc cgaggccttg ggctatacct gggacaaggt
     1621 ccacgaggag gccgagcggc tggagcacgt catttccgag gagttggagg agaagatctt
     1681 ccaggcgttg gggcggccca cccgggaccc gcatggggag cctattcccg gtcgggacgg
     1741 aactctggcg gcggtggaga tcgagccgct ctccgagctg gctgcgggaa ccgcgggagt
     1801 catccgtcag gtcagcgaca gtcatgcgga gatgcttcgg tatctcgggg cgcggggcct
     1861 ggtgccggat gcggcggtcg agatcctgga cagggcgccc ttcaacggac ccatcacggt
     1921 aaggataggc aaggtctctc acatgctagg gcgcgaactt gcggggcata tctgggttca
     1981 aatcagcacg aatggagtgt cctgaccggt tcgggagctg tcggtggtcg gagaggtttt
     2041 ggggtgtgag tcatggccga caatgacgcg ttgactgaag ggcaaacatc ttctcccgaa
     2101 tcttcaacca gcgtattgtc gggcgacgag cggaccctcg ccgccgcggc ggaggtgttg
     2161 gagggccgaa gccacgaggc ctggcaccgc cgcctcttgc cgttcctggg accggccttc
     2221 atcgccagcg tggcgtacgt ggatccgggc aacttcgcca ccaacatcca gggcggcgcc
     2281 cagttcggat acctgctgct ctgggtgatc gtcgccagca atctgatggc catgctggtg
     2341 caagcgcttt ccgccaagct ggggatcgcc accgggagga acctggccga gatgtgccgc
     2401 gaccagtttc cccgctgggt cgtttggacc atgtggggtc tgatggagat cgtggccatg
     2461 gccacggact tggccgagtt cctgggtgcg gcgctaggat tcaacctgct gttcggcatg
     2521 ccacttttcg tcgcgggcct gctcacgggc gtcgcgacct ttgccatcct ggcgctggag
     2581 cggcacgggt tccgccctct cgaggcgatc atcacctccc tggtcggggt catcgccttc
     2641 tgctacctga tcgaaacctt cctcgggcga cccgactggg ggcagatcgt ctaccactct
     2701 ttggtgccaa ccttcagcgg ggcagagagc gtgattctcg ccaccggcat cttgggcgcg
     2761 accgtgatgc cccacgtggt gttcctgcac tccgccctaa cccagggacg catcgtgacg
     2821 cgtaagcccg agcagttgcg ccgcctgttt cgctacgagt gcgtggatgt gaccatcgcc
     2881 atgggtgtgg ccggattggt gaacggggcg atgctagtca tggccgccgc cacatttttc
     2941 cagaaaggtt tgaccactgt ggggacgatt gagcaggcgc atcagacctt ggtgccgctt
     3001 ctgggtcgag cctcgagttg ggtctttgcc atctccctcc tggcatccgg tctgtcctcc
     3061 tcaaccgtgg gaaccatgtc cgggcaaatc atcatgcagg gcttcgtgca cttcgggatc
     3121 ccgatctggc ttcgccggtt gctcacgatg ctgccggcgc tggttgtgat cgcggtcggc
     3181 atggagccga cgcgcacgct agtcctcagc caagtggtgc tgagcttcgg tctgcccttt
     3241 gccctggtgc ccctggtgct gttcacggcg cgatccgatt tgatgggagt cctggtcaat
     3301 cgccggctca cgactctctt ggccagcctg gtgacgctcc tgatcattgc gttgaacctc
     3361 ttcctgctgt atcagatcat ctgg
//
LOCUS       DBNGZC010000995         2203 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_769608, whole genome shotgun sequence.
ACCESSION   DBNGZC010000995 DBNGZC010000000
VERSION     DBNGZC010000995.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_769608"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="AC16G0_17115"
     CDS             complement(<1..293)
                     /locus_tag="AC16G0_17115"
                     /inference="COORDINATES: protein motif:HMM:NF013460.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101377.1"
                     /translation="MFRRGHAAGIQRFTANERLQHALLMVSILILLVTGFSLLFHDLW
                     IGRWLLWLEGGIEGRGLIHRWAAGVLMAVCGHHVLYVLFTERGHEQFMQMRPR"
     gene            complement(337..1599)
                     /locus_tag="AC16G0_17120"
     CDS             complement(337..1599)
                     /locus_tag="AC16G0_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101378.1"
                     /translation="MGGAIALGLLVFAAAAGAAPAGKAVAVADRECLRCHGDPALTRV
                     QPGERGRTMFVDQAQLARSRHAKVACTACHAGITRLPHVPKLSRAECGGCHQREATAY
                     AGSIHGRGVAKADVDVPTCQRCHGGHDIVAAADPASRVSPLRQGQTCMECHADAKITT
                     RHPDMPNILLIRAYADSVHGRAVAKKGLAVAPSCTGCHGTHDLKPADDPGSPVHRANV
                     AQTCGRCHQGILATYRDSIHAVALAKGVADAPTCTGCHGEHTIAAPTDPKSRVAPGNI
                     SKTCGACHGAERIVKAYGLATKREQTYADSFHGIAVKFGNVEAANCASCHGFHDIRPS
                     SDPRSRVAKANLPATCGSCHPGAGARFAEGRVHIEKTKESAPGVFYVRTFYIWFIGIL
                     MVCFLGYMTMEIVGYRRRSRLGSPPEGR"
     gene            complement(1709..>2203)
                     /locus_tag="AC16G0_17125"
     CDS             complement(1709..>2203)
                     /locus_tag="AC16G0_17125"
                     /inference="COORDINATES: protein motif:HMM:NF013460.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HIR5101379.1"
                     /translation="SHLLAMLPRFEDVKEVVQNLGYLLGLRTEPPRFDRFSYIEKAEY
                     WALIWGSVVMAATGFMLWFENQTLQYFPKWFTDLATIVHYYEAWLATLAIIVWHIYYV
                     IFNPDVYPMNWTWLTGKVSGQMLRHEHPREYERLCQREEVDVAGGGSEASDSGLGEAP
                     ERAG"
BASE COUNT          385 a          750 c          691 g          377 t
ORIGIN      
        1 caccggggcc gcatctgcat gaactgctcg tggccacgct ccgtgaacag gacatacaag
       61 acatgatgcc cgcacaccgc catcaggacc ccggcggccc agcggtggat cagcccgcgc
      121 ccctcgatcc ctccttccag ccagaggagc catcgtccga tccagaggtc atggaagagc
      181 aggctgaacc ccgtgaccaa caggataagg atgctgacca tgaggagcgc gtgctgcaag
      241 cgctcgttcg ccgtgaacct ctgaatccct gcggcatgac ctcgcctgaa caccttgacc
      301 ccctacccct gtgtcgaccc cggtcacgcg tcggtgtcac cgaccctcag gaggactccc
      361 caggcggctg cggcgacggt acccgacgat ctccatggtc atgtagccga ggaagcagac
      421 catcagaata ccgatgaacc agatgtagaa tgtccggaca tagaagacac ccggggcact
      481 ctccttggtc ttttcgatgt gcacccgccc ctcagcaaac ctggctcccg ctccggggtg
      541 acacgaaccg caggtggccg gcagattggc cttggcaacg cgggagcggg gatcgctgga
      601 cgggcggata tcgtggaagc cgtggcaact ggcacaattg gccgcttcga cattgccaaa
      661 cttgacggcg atcccgtgga aactgtcggc gtaggtctgc tccctcttgg tcgccaggcc
      721 atacgcctta acgatgcgct cggccccgtg acaggcgccg cacgtcttcg agatgttccc
      781 cggcgccact cgagacttcg gatccgttgg cgcggcaatg gtgtgctcgc cgtggcagcc
      841 ggtacaagtc ggagcatcgg caacgccctt ggccagggcc accgcatgga tgctatcgcg
      901 gtacgtcgcc aggatcccct ggtgacaacg cccgcaggtc tgtgccacgt tcgcgcggtg
      961 cacgggggag ccgggatcat ccgcaggctt gaggtcatgg gtcccgtggc agcccgtgca
     1021 cgagggcgcc accgcaaggc ccttcttggc cacggcccgt ccgtgcacgc tgtcggcata
     1081 ggcgcgaatc agaaggatgt tcggcatgtc cggatgtcgg gtcgtgatct tggcatccgc
     1141 gtggcattcc atgcaggtct gcccttgacg gagcggagac acccgcgatg ccggatccgc
     1201 cgcggccacg atgtcgtgcc caccgtggca gcgctggcag gtcgggacgt ccacatctgc
     1261 cttggccacg ccccgcccat gaatgctgcc ggcgtaggct gtcgcctcgc gctggtgaca
     1321 gcccccacac tcggcccgac tcagcttggg tacgtgcggg agcctggtga tcccggcatg
     1381 gcaggccgtg caggccacct tggcatgacg tgagcgggcg agctgagcct ggtccacgaa
     1441 catggtccgc ccccgctcgc cgggctggac gcgggtaagc gcagggtccc catggcaccg
     1501 caggcactcg cggtccgcca cagcaaccgc tttccccgct ggggctgccc ccgccgccgc
     1561 tgcaaagacc aggaggccaa gagctatcgc cccgcccatc cccaggatgg ctgcagaggg
     1621 ggtccgatat ctggcccttc gcgagggaca gtccccgtcc gtcgggcctg gggtgccgcg
     1681 ggccatccgt ctcctcacgc cgcttcgttt atccggcacg ctctggtgcc tccccgagtc
     1741 cgctgtctga ggcttcactc cccccaccgg cgacgtcgac ctcttcccgc tggcagagcc
     1801 gctcatattc ccggggatgt tcgtgccgga gcatctgccc cgagactttc ccggtgagcc
     1861 aggtccaatt catcgggtat acgtccgggt tgaagatcac gtagtagatg tgccacacga
     1921 tgatggccag ggtggccagc cacgcctcgt agtagtggac gatggtggca aggtccgtga
     1981 accacttggg gaagtactgc agcgtctggt tctcgaacca gagcatgaaa cccgtcgctg
     2041 ccatcaccac cgatccccag atcaaggccc agtactccgc tttctcgatg tagctgaacc
     2101 ggtcgaagcg cggaggctca gtccggaggc cgaggagata cccgaggttc tggaccacct
     2161 ctttgacatc ctcgaagcgc ggcaacatgg ccaggagatg gga
//
LOCUS       DBNGZC010000996         1659 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1156606, whole genome shotgun sequence.
ACCESSION   DBNGZC010000996 DBNGZC010000000
VERSION     DBNGZC010000996.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1156606"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1023)
                     /gene="rlmKL"
                     /locus_tag="AC16G0_17130"
                     /pseudo
     CDS             complement(31..1023)
                     /gene="rlmKL"
                     /locus_tag="AC16G0_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004576445.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
     gene            complement(1060..1365)
                     /locus_tag="AC16G0_17135"
     CDS             complement(1060..1365)
                     /locus_tag="AC16G0_17135"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR5101380.1"
                     /translation="MASSGGGLGGSGGIGDLLQSIRRRIEQAKTYPDAARRAGMEGTV
                     EVRFRIGREGSAEGLEIVHSSGYPELDEIAMQTIRRAGPYPAVSGRIRIPLSYRLDR"
BASE COUNT          293 a          557 c          544 g          265 t
ORIGIN      
        1 ctccatagac tcaagggggc ggggcgatga tcagggatgg cgcgtgatgc gccagcaggt
       61 gtggatgcgg gggttacggg cgaagtcgtg cgggatggtg gcgggcgata ggtcctccaa
      121 gtggaggtcg gagagcgcgg ggtgatccag cttgaagcgc tggaagtttg tggagaacag
      181 caggacgccc ttcggggcga ggagatccgc cgtggcgcgg atgagcggga tgtgatcgcg
      241 ctgcacgtcg aaggtgcgct cgcccatgcg cttggacgtg gagaaggtcg gcgggtccaa
      301 gaagatgagg tcataccgct cgggccggcg agcctccacc cacgtgagac agtcggcgtg
      361 cacgaagcga tggaccgccc cctcgacgaa accgttcaga accatgttgc gccgcgccca
      421 gtcaagatag acccgggaca tgtccacgct ggtcgtggat acggcgccac ccgaggcggc
      481 gtacacgctc gccgtgccgg tgtaggcgaa cagattcagg aagtgcaggc ccagcgccaa
      541 ctcgcggatc atcgcccgcg tgggccgatg gtccaggaaa aggcccgtat ccaggtagtc
      601 cgtaaggttc accaggaatt gcagactacc ctcgctcacc tcgctgaagt cgcccgtcgt
      661 tgcctgacgc tcgtactggg ccagaccctt ctgcctgcgg cgcaccttga gcaccacgtt
      721 cctggccgga atgccaagca cctccgggag gaccgccatg gcttcgcgta acctggcctc
      781 ggcacgtgcg ggagccaccg tcgccggcgc cgcgtactcc tgcacgtgcg cccacccctc
      841 gtagaggtcc acggccaggg cgtactccgg catgtcggca tcgtagagcc gatagcaggt
      901 gataccctcg cggcgcgccc acttgccgag cagtcgcagg ttcttccgca atcggttggc
      961 gaacatctcc gctccggctg gcggagctga aggctgatct ttagcagggg tcacggattt
     1021 ggccccttga atcgacgaaa gttcatgagg aagtcgtccc tagcggtcca gacggtagga
     1081 gagcggaata cggatgcggc cgctcaccgc cggatacgga ccggcgcgcc ggatggtctg
     1141 catggcaatc tcgtccagct cggggtaccc cgaggaatgg acaatctcta gcccctccgc
     1201 actgccctcg cggccgatac ggaagcgaac ctccaccgtt ccctccatgc cggcgcgccg
     1261 cgccgcgtcg ggataggtct tggcctgctc gatccggcgc cggatggact ggaggagatc
     1321 gccgatgcca ccgctgccac caagaccacc tccactcgag gccatcccgg atcccccgcc
     1381 tttgcccgcg ccgttcccgg agccgccacc cgcatccttt ccagcgccct gtccctccct
     1441 gtcggtccct ccaccagcga aacccgaccc acgattcgat gtgtcaatac catcgcggcc
     1501 caacccgctc ccacttccgg caggaatcag gaagatcccc gcgatctcag cctcgggctt
     1561 cggcgctgga ggcacgggag cggcttcggg gacagaggtc gcaggccccg cccgaagccg
     1621 ggtaacatca ctctgacccg ctccgggcgg tccggagag
//
LOCUS       DBNGZC010000997         2805 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1261512, whole genome shotgun sequence.
ACCESSION   DBNGZC010000997 DBNGZC010000000
VERSION     DBNGZC010000997.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1261512"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1553
                     /locus_tag="AC16G0_17140"
     CDS             165..1553
                     /locus_tag="AC16G0_17140"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006456728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5101381.1"
                     /translation="MNAKHQVIILGGGFGGLYAAQALRSAPVDVTLIDRRNYHLFQPL
                     LYQVATGSLSPGEIAAPLRGVLSHQQNTRVLLGEVEDIDPGAKRVVLRDGEQLAYDSL
                     IVATGSQSFYFGNEPWREWAPSLKSVEEATTIRHKILYAFEAAERVCEPGARSSWLTF
                     VIVGAGTTGLELAGALAEIARKTLRNNFRCIRPEEAQIILLDGSPRVLPTYAEDLSAK
                     AEQALQRLGVQVRLKVHVTGIDAEGVTFQTPDGTERLAAKTVLWAGGITASAFGRILA
                     NRARAETDKGGRIQVAPDLTLPQYPDIYVVGDLALVLRPDGRPLPGVAPVAMQQGAYA
                     AKAILRRLEGRTKLPPFRYVDKGSMAVIGRASAVADLFGVHLSGFPAWLIWLFVHLMY
                     LVEFQSRILVLIQWGFQYLTFWRGARLITGHAASDSIFTDGAVRPVPELSAGQPVGPD
                     RRAGRSEHGEKL"
     gene            1537..2496
                     /gene="mbfA"
                     /locus_tag="AC16G0_17145"
     CDS             1537..2496
                     /gene="mbfA"
                     /locus_tag="AC16G0_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron exporter MbfA"
                     /protein_id="HIR5101382.1"
                     /translation="MGRNFRDLSEREILALAISLEEEDGRIYGDFADGLRDSYPVTAK
                     VFEEMQAEEAGHRNSLIETYRQRFGEHIPLIRRQDVKGFVDRRPEWLVRPLGIKVVRK
                     QAEIMELETRRFYERAAQQITDASVRKLLDDLAEVERRHSSLAESLGKERLTPDVRTE
                     EEAAERRLFVLQIVQPGLAGLMDGSVSTLAPLFAAAFATRSSWAAFLVGLAASIGAGI
                     SMGFAEALSDDGSLTGRGHPWLRGVVCGLMTTAGGIGHTLPFLIPSFQVATTVAVVVV
                     ALELAAISYIRHRYMDTPFLSAAFQVVVGGVLVFLTVILIGSS"
BASE COUNT          451 a          873 c          963 g          518 t
ORIGIN      
        1 ggtgggttcg cgagggattc cacccatcga gtctgcaggg ccgcccctga gcgggaattc
       61 cttccggatg gcatcatctt caagctgctg agcgtccttg gcgaaggaac gctgaaattg
      121 ttccaactcg cgcgcgctct cgccccgcct gggtggaggt atgtatgaat gccaagcacc
      181 aggtcatcat cctggggggt ggttttggcg ggctgtatgc ggcgcaggcg ctccgatcgg
      241 ctccggtgga tgtcaccctc atcgatcgac gcaactatca cctcttccag cccctgctgt
      301 accaggtggc caccggctcg ctctcgccgg gggagatcgc ggcgccgctc cgcggcgtcc
      361 tgagtcacca gcagaacacg cgcgtcctgc tgggtgaggt cgaggacatc gatccggggg
      421 caaagcgggt ggtgctgcgg gatggggaac agctcgccta cgattcgctg atcgtggcca
      481 ccggctcgca gagcttctac ttcggcaacg agccgtggcg ggaatgggcg cccagcctga
      541 agagcgtgga ggaggcgacg accatccgcc acaagattct gtacgccttt gaggccgcag
      601 agcgggtatg tgagccgggt gcgcgctcct catggctgac cttcgtcatc gtcggcgccg
      661 ggaccacggg tctcgagctg gcgggtgccc tggcggagat cgcccggaaa acgctcagga
      721 acaacttccg ctgcatccga ccggaggagg cgcagatcat cctgctggac gggtctcccc
      781 gggtcctccc gacctatgcg gaggacctct ctgccaaggc ggaacaagcg ctgcagcggc
      841 tgggtgtgca ggtgcggctg aaggtgcacg tcacgggtat cgacgcggaa ggcgtcacct
      901 tccagacgcc ggacggcacc gagcgactcg ccgcgaagac cgtcctgtgg gccgggggaa
      961 tcacggcgtc ggccttcggg agaatcctcg ccaatcgcgc ccgggcggag acagacaagg
     1021 gtggtcgaat ccaggtggca ccggatctca cgctgccaca gtaccccgac atctacgtcg
     1081 tcggggatct ggccctggtc ctgcgccccg atggcaggcc gctgccgggc gtggccccgg
     1141 tggcgatgca gcaaggggcc tatgccgcga aggcgatcct gaggcggctg gagggccgca
     1201 cgaagctgcc gccgttccgg tacgtcgaca aggggagcat ggcggtcatc ggccgtgcat
     1261 cggcagtggc cgatctcttc ggcgttcacc tctcggggtt tcccgcatgg ctgatctggc
     1321 tgttcgtgca tctgatgtac ctggtggagt tccagagtcg catcctggtc ctcatccagt
     1381 ggggctttca gtacctgacc ttctggcgcg gggcacggct gatcacggga catgccgcgt
     1441 cggactccat ctttacagat ggggccgtgc ggcccgtgcc ggagctatcg gcaggccagc
     1501 cggtcggccc cgaccggcgt gcggggagga gcgagcatgg ggagaaactt tagggatctc
     1561 tccgagcgcg agattcttgc cctggcgatt tccttggagg aggaggatgg acgcatctac
     1621 ggcgacttcg ccgacggtct ccgggacagc tatccggtga cggccaaggt cttcgaggag
     1681 atgcaggccg aagaggcagg gcatcggaac agtctgatcg agacctaccg acagcgcttt
     1741 ggcgagcaca ttcccctgat ccgccgccaa gacgtgaagg gctttgtgga ccggcgcccc
     1801 gagtggttgg tgcggccgct gggaatcaag gtcgtcagga agcaggcgga gatcatggaa
     1861 ctggagaccc gtcggtttta cgaacgagcc gcacagcaaa tcaccgatgc ctcggtgcgc
     1921 aagctcctgg atgacttggc cgaggtcgaa cgccgacact cctccctggc ggaatccctt
     1981 ggaaaagaac gcctcacgcc tgacgtgcgg acggaagagg aggccgcaga gcgccggctg
     2041 ttcgtgctcc agatcgtcca accgggtctg gcggggctga tggacgggtc ggtctcgacc
     2101 ctggcaccct tgttcgccgc cgccttcgcc acgcgcagta gctgggcggc cttcctcgtg
     2161 ggcttggccg cctccatcgg ggccgggatc tccatggggt ttgccgaggc cctgtcggat
     2221 gacggctctc tgacggggcg gggtcatcct tggctccgcg gagtggtctg cgggctgatg
     2281 accacggcgg ggggcatcgg ccacaccttg cccttcctca tcccgagctt ccaggtcgcg
     2341 acgaccgtcg ccgtcgtggt ggtggccctg gaacttgccg ccatcagtta tatccgacac
     2401 cggtacatgg acacgccatt cctctccgcg gcgttccagg tcgtcgtggg tggggtgctg
     2461 gtgttcctga cggtgatcct catcggcagt tcgtgatggt ttgaccccgc gacacctcag
     2521 cctcactgag agtgtctgtt gatcagggac actcaggcgt atgacgcatc cccatcgccc
     2581 tcgcatgacc gggcaggtct cacattgtct ccggtgatgc ctggatcgtt ggcagcggtg
     2641 cttctccttg acgcccggta cgtagcgccc tagactccgg ctcccatcga gatgttttgc
     2701 ccggtgggct gtaccgcgac gtacgtgata cgaccggcgc atcttctcgc caacgcggga
     2761 gggggagacg gtggaagagc aggctgtcct tgagcgcctg cgcaa
//
LOCUS       DBNGZC010000998         1719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2237610, whole genome shotgun sequence.
ACCESSION   DBNGZC010000998 DBNGZC010000000
VERSION     DBNGZC010000998.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2237610"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="AC16G0_17150"
     CDS             complement(<1..789)
                     /locus_tag="AC16G0_17150"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013528.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR5101383.1"
                     /translation="MTGLGGVKRWRIADADPTSADALALALNVSSLLAALLIRRGCNT
                     AEAAQTFLDAPLTALEDPWQMAGMVAAVDRLRAAVARREPILVCGDFDVDGVSGTAIL
                     VGGLQRAGAEVVYALPRRLEHGYGLPASIVEEAAAKGIQLLVTVDQGITAHEAVALAR
                     VRGLDVIVCDHHLPAPSLPPATAILNPRRPDCSYPFKDLGGAGSAFKRLQALFGPGAS
                     EELLPFLDLVALGTIADLVPLLGENRILVTHGLAQLAATERPGIR"
     gene            complement(809..1717)
                     /gene="secF"
                     /locus_tag="AC16G0_17155"
     CDS             complement(809..1717)
                     /gene="secF"
                     /locus_tag="AC16G0_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIR5101384.1"
                     /translation="MGKTNFDFIGKRHITYIISTVLVLTGIVALIQISRGAANMGIDF
                     AGGTSIQVKFTQPLDLGVVRDALSRNGIKDSEPQQFADGRSVLIRLKRSGSEGTSLSD
                     QVQAALKKEIANNSFTVEASSEIGPAIGKDLQQKALLAIVMSLAGIVVYIAWRFKFDF
                     GVAATIATFHDVLAVLGVVFILHKEITLLIITALLTIGGYSLTDTVVVYDRIRENLRS
                     RRKEQLADTINASINQVLSRTAMTSLTTLLAALALFLLGGEVLHDFAFALLIGIVVGT
                     YSSWFVASPLIYEWRIRTESTRRGRK"
BASE COUNT          305 a          542 c          593 g          279 t
ORIGIN      
        1 gcggatgccg gggcgttccg tggccgccag ttgggcgagc ccatgcgtca ccaggatgcg
       61 attctccccc aggaggggga ccagatccgc gatcgtgccc agggcgacga gatccaggaa
      121 cggcaggagt tcctcgctgg cccccgggcc aaacagggcc tggagccgct tgaaggcgct
      181 ccccgctccg cccaggtcct tgaatgggta ggaacagtcc ggccggcgtg ggttgaggat
      241 ggccgtggca ggaggcaggc tgggcgccgg caggtgatga tcgcagacga tcacatccag
      301 tccccggacc ctggccaggg ccactgcctc gtgcgccgtg atcccctgat ccaccgtgac
      361 caagagctgg atgcccttgg cggccgcctc ctccacgatg gaagccggaa ggccatagcc
      421 gtgctcgagg cgacggggca aggcgtagac cacctcggcc cccgcgcgct gtagcccccc
      481 cacgagtatg gccgtgccgc tcacgccatc cacgtcgaag tcgccgcaga cgaggattgg
      541 ctcccgccgt gctacggcgg cgcgcaagcg gtccacggcg gcgaccatcc ccgccatctg
      601 ccagggatct tccagggccg tgaggggagc atccaggaag gtctgcgcgg cttcggccgt
      661 gttgcagccg cgccggatca agagagccgc caggagggag gagacgttca gtgccagggc
      721 cagggcgtcg gccgaggtcg ggtcggcatc cgcgattcgc cagcgcttga cgccgcccag
      781 acccgtcacg ggtagccgcg tccccggcct acttgcgacc gcggcgagtg gattcggtcc
      841 ggatccgcca ctcgtagatt aggggcgagg ccacgaacca ggaggagtat gtccccacca
      901 cgattccaat caggagggca aaggcgaagt cgtgcagaac ctccccgccc aagaggaata
      961 aggccagggc cgcgagcaag gtggtgaggg acgtcatggc cgtccggctg agcacctggt
     1021 tgatgctggc gttgatggtg tcggccagtt gttctttgcg gcggctgcgg aggttttcgc
     1081 gaatgcgatc gtacaccacc accgtgtccg tcagagaata tccgccgatg gtcaaaagcg
     1141 ccgtgatgat gagcagcgtg atctccttgt gcaggatgaa cacgacaccc agaaccgcca
     1201 ggacgtcgtg gaaggtggcg atggtggcgg ccacgccgaa gtcgaacttg aaccgccagg
     1261 cgatatacac cacgatcccg gccagggaca tgactatggc gagcagggcc ttctgctgaa
     1321 ggtccttgcc gatggccggg ccgatttcgc tcgaggcttc caccgtgaag gaattgttcg
     1381 cgatctcctt cttcagggcg gcctggacct gatccgacag gctggtgcct tcggacccgc
     1441 ttcgcttgag gcgaatgagc acgctgcggc cgtcggcaaa ctgctggggc tcgctgtcct
     1501 tgatcccgtt cctggagagg gcgtcgcgca ccaccccgag gtccaggggc tgggtgaact
     1561 tgacctggat agaggtcccc ccggcgaagt cgatgcccat gttggcggcc ccccgggaga
     1621 tctggatcag ggccacgatc cccgtcagga ccaggacggt cgagatgatg taggtgatat
     1681 ggcgcttgcc gatgaaatcg aagttggtct ttcccagga
//
LOCUS       DBNGZC010000999         6446 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2040298, whole genome shotgun sequence.
ACCESSION   DBNGZC010000999 DBNGZC010000000
VERSION     DBNGZC010000999.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6446)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2040298"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="AC16G0_17160"
     CDS             complement(<1..458)
                     /locus_tag="AC16G0_17160"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008514453.1"
                     /note="catalyzes the formation of 5-phospho-alpha-D-ribose
                     1-diphosphate and nicotinate from nicotinate
                     D-ribonucleotide and diphosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIR5101385.1"
                     /translation="MSESLFHTATDAEIKAGQITDVYFERTLQILNRLGITKRVVAEV
                     ILKSFPPGWTWGVLAGIEEVAALLTGLPVTVHTFLEGSIFGTMQPVLTIEGRYVDFAQ
                     YETALLGLLCQASGVATKAARCKKAAAGHPVVSVGARRMHPALAPMIERNA"
     gene            complement(511..1413)
                     /gene="rsmA"
                     /locus_tag="AC16G0_17165"
     CDS             complement(511..1413)
                     /gene="rsmA"
                     /locus_tag="AC16G0_17165"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR5101386.1"
                     /translation="MTQAPPRAPSLSSRTRALLAARGLHPRRARGQHFLIDPRVRDSI
                     VAAAGVGPGDTVLEIGPGTGVLTEALLGVGASVLALEVDRDLAVLLREQLADETGLTL
                     WIGDALRFDFAAHLGDHPARGAIQVVANIPYNITSPLILRLLKCRELFSALHLTVQRE
                     VAERLSAAPGTKAYGALTLACQYWASVRRVLPIRRTAFYPAPEVDSTLVRFDLLEAPR
                     VRVESAEQLFGVIRAAFGQRRKMLRNALGHGGWRPAAVDAALAMSGIAGARRGETLAL
                     EEFAELCEALPARAGGTDRQGEVG"
     gene            complement(1410..1556)
                     /locus_tag="AC16G0_17170"
     CDS             complement(1410..1556)
                     /locus_tag="AC16G0_17170"
                     /inference="COORDINATES: protein motif:HMM:NF024820.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5101387.1"
                     /translation="MNLGLLYQKAGNLTEAVQTWERALQLNPKHPLARIYLSQATASG
                     TSVE"
     gene            complement(1557..2123)
                     /locus_tag="AC16G0_17175"
     CDS             complement(1557..2123)
                     /locus_tag="AC16G0_17175"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5101388.1"
                     /translation="MSEVRRILRQGTAAFEAGDYDKAEGLLGEVLSQEPAFASVYNML
                     GVIAGHKNAPQKAAALFRQALGLNPDYREAQVNLAITLAEMGAYDQAAQTVGDLEQRE
                     TREETRLSPGVLGKLANAHADLGRKYHALGLYAQAILEFDKALELCPAFPDIHNRRAL
                     SCRELGDHAGAQASLLRALQLNPNYVEA"
     gene            complement(2172..3113)
                     /locus_tag="AC16G0_17180"
     CDS             complement(2172..3113)
                     /locus_tag="AC16G0_17180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR5101389.1"
                     /translation="MAMHACDGFGQRGRHTRLVGVLLLAVTAALPLQGCMIYDYLYGG
                     GGSTRRETQRSDQDLLRSAESQLQRNQYEDARKDLQRLMNQYPDSDLVSVARLASAKA
                     LYMQRKYDEARAEYQRFLDLHPQHERADEAHYYLGMTYFRQSDTPDRDQTFTRKALDE
                     FELLITQMPDSQYVPDARERTSVCRRKLAEKEVYVGTFYYTRGNYAAAAGRFTAMLAQ
                     YGGAGFDDQALYYLGESLWQLEQKAEARAAFQRLVQDYSQSEWAAPAARRLGVTLVRT
                     GPPKPEGPGMFSSMWLGMQQTWEEVVDTVKGYSIFRR"
     gene            complement(3307..4554)
                     /gene="clpX"
                     /locus_tag="AC16G0_17185"
     CDS             complement(3307..4554)
                     /gene="clpX"
                     /locus_tag="AC16G0_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR5101390.1"
                     /translation="MAKAVKSGSKLQCSFCGKSQDDVRKLIAGPTVYICDECIELCND
                     IIAEEWEEEKSKGFSQLPKPAEIKAILDQYVIGQERAKKTLSVAVHNHYKRINARQSF
                     DDVELSKSNILLIGPTGSGKTLLAQTLAKILDVPFTIADATTLTEAGYVGEDVENIIL
                     RLLQVADYDVERAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPQQEYLQVDTTNILFICGGAFVGLDKIIQGRVGERRVGFGAQVHGRRDR
                     KLSEVLGELQPEDLLRYGLIPELVGRLPVAAILHELDEKLLVRILKEPKNSLLRQYQK
                     FFDFEHVKLTFTEDAVAAVAEEAAKREAGARGLRAILEDIMLDIMYELPSQSGISECI
                     INRDVVIERKHPIILYKKKAESA"
     gene            complement(4607..5266)
                     /gene="clpP"
                     /locus_tag="AC16G0_17190"
     CDS             complement(4607..5266)
                     /gene="clpP"
                     /locus_tag="AC16G0_17190"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009370198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HIR5101391.1"
                     /translation="METYLVPRVVEQTSRGERAYDIFSRLLKDRIIFIGMPIDDAWAN
                     LVIAQLLFLEAEDPDKDIHLYINSPGGSVTASLAIYDTMQFVKPAIETICMGQAASGA
                     ALLLAAGTKGKRMALPHSRIMIHQPYGGAQGQAVDIQIQAKEILRMREELNQIIARHT
                     GQPLERVEKDSDRDFFMSPEEARVYGLIDEVIFYRDLAKKPASGDIALPAAAEGAALK
                     A"
     gene            complement(5349..>6446)
                     /gene="tig"
                     /locus_tag="AC16G0_17195"
     CDS             complement(5349..>6446)
                     /gene="tig"
                     /locus_tag="AC16G0_17195"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR5101392.1"
                     /translation="ELIPDSYREALAQAALQPVSQPQVDEVHFHEGEPLRYRAVVEVK
                     PSVEVRDYRGIPLSRDKIAVADQEVDRALEYLREEAAEYVPMEGWPAVRDDLVILDHE
                     GSVHGKPFKGGSGKNMALILGQEGYLPGFSEQILGMQKGDAKRFALPFPEDIPRKDLA
                     GRTAEFKVTVKEVKKRRLPELNDEFAKSAGDVDSVPALRDKLRQDILARKGREQESGL
                     KRALLEKLAAAHELEVPEAMVEAEASSILQDMLMTLRATGGRIEGLARDPEALRAKAS
                     EAGRKRAKESLLLEAVAKQEGLTVSEAEIEGEIEAMARAYRQEPAVARQALEDPVRRA
                     SLTARLLQRKALDLLFQQATITESYNLITPA"
BASE COUNT          971 a         2232 c         2062 g         1181 t
ORIGIN      
        1 gcgttccgct cgatcatcgg cgccagggct ggatgcatgc gtctggcccc gacgctcacc
       61 accgggtgcc ccgccgccgc cttcttgcac cgggcggcct tggtcgccac ccccgaggcc
      121 tggcacagga gccccagcag ggccgtctcg tactgggcga aatccacgta gcgcccctcg
      181 atggtcagga ccggctgcat cgtcccgaag atgcttcctt cgagaaaggt gtggaccgtc
      241 acgggcaagc cggtcaggag ggccgcgacc tcctcgatgc cggccaggac cccccaggtc
      301 cacccggggg ggaagctctt caggatcacc tcggccacca cccgcttggt gattcccagg
      361 cgattgagga tctggagggt gcgctcgaaa tagacgtcgg tgatctgccc ggccttgatc
      421 tccgcatccg tggccgtgtg aaacagcgat tcgctcatgg gttctcccgt cggctgatag
      481 ggctttccgg cagcgacagc gatcgggacc tcacccgact tcgccctgcc tgtcggtccc
      541 tccggccctc gccggcagcg cctcgcagag ttccgcgaat tcctcgaggg ccagcgtctc
      601 tccacgccga gcgccggcga ttccgctcat cgccagcgcg gcatccaccg ccgccgggcg
      661 ccagcccccg tgaccgaggg cattccggag catctttcgc cgctggccga aggccgcccg
      721 gatcaccccg aagagttgct cggcgctctc cacccgcacc cgcggcgcct ccagcagatc
      781 gaatcgcacc agcgtcgaat ccacctccgg ggccgggtaa aaggcggtcc gccggatggg
      841 caggacacgg cgcaccgagg cccagtactg gcaggccagg gtcagggcgc cgtaggcctt
      901 ggtccccggc gcggcgctga gtcgctccgc cacctcgcgc tgcacggtga ggtgcaacgc
      961 cgagaagagt tcccggcact tgagcagtcg gaggatcagg ggactcgtga tgttgtacgg
     1021 gatgttggcc accacttgga tggccccccg cgctggatgg tccccgaggt gcgccgcaaa
     1081 gtcgaaacgc agggcgtccc cgatccacag ggtgagaccc gtctcgtccg ccagctgctc
     1141 gcgcagaagg acagccagat cccgatcgac ctccagcgcc agcacgctgg ccccgacgcc
     1201 aagcagtgcc tccgtgagga cgcccgtgcc gggtccgatt tccagcaccg tgtcgccggg
     1261 acccacaccg gccgccgcca cgatggagtc ccgcacgcgt gggtcgatca ggaagtgttg
     1321 tccgcgcgcc cgacgcgggt gaaggccgcg ggccgcgaga agcgctctcg tgcgggagga
     1381 gagcgagggg gcacgtggag gcgcctgggt cactcgacgg aggtcccgga ggccgtggcc
     1441 tggctcaggt agatgcgggc gagcggatgc ttgggattga gctgcagggc gcgctcccac
     1501 gtctgcaccg cctcggtcag gttccccgcc ttctggtaga gcaggccgag gttcacctaa
     1561 gcttcaacgt agttcgggtt gagctgcaat gcgcgcagca gcgaggcctg tgcgcccgca
     1621 tgatctccca gttcgcggca actcagcgcc cggcggttat gaatatccgg gaatgccggg
     1681 cacagctcca aggccttgtc gaattccagg atcgcctggg catacagtcc cagggcgtga
     1741 tacttccgcc ccaggtcggc gtgggcgttg gccagcttgc ccaagacgcc gggactgagg
     1801 cgcgtctcct ccctcgtctc gcgctgctcc agatcgccga ccgtctgcgc ggcttgatcg
     1861 taggccccca tctccgccag ggtgatggcc agattgactt gggcttcccg gtagtcgggg
     1921 ttgaggccga gggcttgccg gaacagagcc gccgccttct gcggcgcgtt cttgtggccc
     1981 gcgatcactc ccagcatgtt gtacacgctg gcgaaggctg gctcttggct caggacctcg
     2041 cccaggaggc cctccgcctt gtcgtagtcg ccagcctcga aggcggccgt gccctgccgg
     2101 aggatccggc gaacctcgct cattcccctc tgctcagcct ctcgcgttta gtctggcgtg
     2161 acgagccgtc cctagcgccg gaagatggag tatcccttga cggtgtccac cacctcttcc
     2221 caggtctgtt gcatccccag ccacatgctg ctgaacatcc cgggcccctc gggcttgggg
     2281 ggaccggtcc ggaccagggt gacccccagc cgccgcgcag ccggcgccgc ccactcggac
     2341 tgggaataat cctggaccag acgttggaac gcggctcgcg cctctgcttt ttgttccaac
     2401 tgccagaggg actcccccag gtagtagagg gcttggtcgt cgaaacccgc cccgccgtac
     2461 tgcgccagca tggcggtgaa acgtcccgcc gcagccgcgt agttcccgcg ggtgtagtag
     2521 aaggtgccga cgtacacctc cttctccgcc agcttccggc ggcagacgct ggtccgctcg
     2581 cgggcgtcgg ggacgtactg ggagtcaggc atctgggtga tgagcagctc gaactcgtcc
     2641 agggcctttc gggtgaacgt ctgatcgcgg tccggggtgt cggactggcg gaagtaggtc
     2701 atccccaggt aataatgagc ctcgtccgcc cgttcatgct gggggtggag gtccaggaag
     2761 cgctggtatt cggcccgggc ctcgtcatac tttcgctgca tgtagagcgc cttggccgac
     2821 gcgagcctgg cgaccgacac caggtcgctg tcgggatact gattcatgag ccgctggagg
     2881 tccttacggg cgtcctcgta ttggttgcgc tggagctggg actccgccga ccggagcagg
     2941 tcctgatcgc ttcgctgggt ttcccgccgg gtgctccccc cgccgccgta gagatagtcg
     3001 tagatcatgc agccctgaag cggcagggct gccgtgaccg ccaggagcag caccccgacc
     3061 aatctggtat gtcgtcctcg ctgcccgaag ccatcgcacg catgcatcgc catcacactt
     3121 cctcctcgga attctcccac gccgagaaag gggatggacc agagcctgct cgccggttga
     3181 cgagccgcgc gcgttgcctg agagggcacg cgacacactc cgggtcctga gaagagtggc
     3241 tgccgaggcg ataggcgcgg cccgagcgaa ccggtccgtc acctcgccgg aaaagcggcg
     3301 gacgcgtcag gcggactcgg ccttcttctt gtagaggatg atggggtgct tccgctcgat
     3361 caccacgtct cggttgatga tgcactcgct gatccccgac tgggagggca gttcgtacat
     3421 gatgtcgagc atgatgtctt ccaggatcgc gcgcagcccg cgggcccccg cctcgcgttt
     3481 cgccgcctct tcagccaccg ccgccaccgc atcctcggtg aaggtgagct tcacgtgctc
     3541 gaagtcgaag aacttctggt actggcgcaa cagcgaattc tttggctcct tcaggatgcg
     3601 gaccaagagc ttctcgtcca gctcgtggag gatggcggcc acgggaagcc ggcccaccag
     3661 ctccgggatc aggccgtagc ggagcaagtc ctcgggctgg agctccccca gcacctcgga
     3721 cagcttccgg tctctccgcc catggacctg ggctccgaag ccgacgcgac gctcccccac
     3781 ccgtccctgg atgatcttat ccaggcccac gaaagccccg ccgcagatga agaggatgtt
     3841 ggtggtgtcc acctggaggt attcctgctg cgggtgtttg cggccgccct gaggcggcac
     3901 gttggccacc gtcccctcca ggatcttcag gagggcctgc tgcactccct cccccgagac
     3961 gtcccgggtg atggaggggt tctccgactt gcgagcgatc ttgtcgatct cgtcgatgta
     4021 gatgatcccc cgctcggccc gctccacatc gtagtcggcc acctgcagca ggcgcagaat
     4081 gatgttctcg acgtcctcgc ccacgtaccc ggcctcggtc agggtggtgg catcggcgat
     4141 ggtgaagggc acgtccagga tcttggccaa ggtctgggcc aagagcgtct tcccgctgcc
     4201 cgtgggaccg atgaggagga tgttcgactt ggagagctcg acgtcgtcga agctctggcg
     4261 ggcgttgatc cgcttgtagt ggttgtggac cgccaccgac agggtcttct tggcgcgttc
     4321 ctggccgatc acatactggt ccagaatggc cttgatctct gccggcttgg ggagctggct
     4381 gaagcccttg gatttctcct cctcccactc ctcggcgatg atgtcgttgc agagttcgat
     4441 gcactcgtcg cagatgtaca ccgtgggacc ggcgatcagc ttgcggacat catcctggct
     4501 cttgccgcag aacgagcact ggagcttgct gccactcttg acggctttgg ccatgcggat
     4561 ctctctcctc gcgccggcgg cgaagccccg agggccggcc gggccctcaa gccttcaggg
     4621 ccgcgccctc ggctgccgcg gggagcgcga tgtctcccga cgccggcttc ttggccagat
     4681 cccggtagaa gatcacctcg tcgatcagcc cgtacaccct ggcctcttcc ggcgacatga
     4741 aaaagtcgcg atcgctgtcc ttttccacgc gctccagcgg ctggccggtg tggcgggcga
     4801 tgatctggtt caactcctcc cgcatgcgca ggatctcctt ggcctggatc tggatgtcca
     4861 ccgcctggcc ctgggccccg ccgtagggtt ggtggatcat gatccgcgaa tggggcagcg
     4921 ccatgcgctt gcccttcgtc ccggccgcca agaggagcgc cgcgccgctg gccgcctggc
     4981 ccatgcagat ggtctcaatc gccggcttga cgaactgcat ggtgtcgtag atggccaggc
     5041 tggccgtcac cgatcccccg ggggagttga tgtacaggtg aatatccttg tccgggtcct
     5101 cggcttctag gaagagcagc tgggcgatca cgagattcgc ccaggcatca tcgatgggca
     5161 tgccgatgaa gatgatccgg tccttcagca ggcgcgagaa aatatcgtac gcgcgctcgc
     5221 cccggctcgt ctgctccacc accctgggaa ccaaatacgt ctccactctt cacctcggat
     5281 ccacccgctc gacggcgggc ggcgtccttc ggctctgcgc cgtgtccctc gccggccctg
     5341 accaaaggtc acgccggcgt gatgaggttg tagctctccg tgatcgtggc ctgctggaag
     5401 aggaggtcca gggccttgcg ctgtaacagg cgagccgtga ggctggcccg acgcaccggg
     5461 tcctccagag cctgccgagc gacggcaggc tcctgccggt aggctcgggc catcgcctcg
     5521 atctctccct cgatctcggc ctcgctcacg gtcagaccct cttgcttggc caccgcctcg
     5581 agcaagagcg actccttggc gcgcttgcgg ccggcctcgc tggccttggc ccggagcgcc
     5641 tcggggtccc gggcgagccc ctcgattcga ccgccggttg cccgcagcgt catcagcatg
     5701 tcctgcagga tggaagatgc ctcggcctcc accatggcct cgggtacctc caactcgtgg
     5761 gccgcggcca gcttctccag aagtgcgcgc ttcagcccgg actcctgctc gcgccccttc
     5821 cgcgccagga tgtcttgccg cagcttgtcc cggagggcgg ggacgctgtc cacgtcgccc
     5881 gcgctcttgg cgaactcgtc gttcagttcg ggcagccggc gcttcttgac ctctttcacc
     5941 gtcaccttga actcggccgt acgccccgcc agatctttcc gggggatatc ctcggggaaa
     6001 ggcagggcga agcgcttcgc gtcccccttc tgcatgccaa gaatctgctc gctgaacccg
     6061 ggcaggtatc cttcctgtcc caggatcagc gccatgttct tcccgctgcc gcccttgaac
     6121 ggtttgccgt ggacgctccc ttcgtggtcc aggatgacaa gatcgtcgcg gacggccggc
     6181 cacccctcca tggggacgta ctccgccgcc tcctcccgaa ggtactccag cgcccggtcc
     6241 acctcctgat ccgcaaccgc tatcttgtcc cgcgacagcg ggatgccccg gtagtcccga
     6301 acctccaccg agggcttcac ctccaccacg gctcgatagc gcagcggctc gccttcgtga
     6361 aagtggactt cgtccacctg cggctggctc acgggttgaa gggccgcctg ggccagggcc
     6421 tcgcggtacg agtcggggat cagctc
//
LOCUS       DBNGZC010001000         1627 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_88041, whole genome shotgun sequence.
ACCESSION   DBNGZC010001000 DBNGZC010000000
VERSION     DBNGZC010001000.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_88041"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="AC16G0_17200"
     CDS             <1..266
                     /locus_tag="AC16G0_17200"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5101393.1"
                     /translation="GTQTSTLVKTPTLEVIRLILQADKEVCHHHEMTGEIMVHCLEGL
                     ITVQINGDSRVLEAGQMLYLAGRESHAIRGLDDASVLLTILFR"
     gene            682..1044
                     /locus_tag="AC16G0_17205"
     CDS             682..1044
                     /locus_tag="AC16G0_17205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR5101394.1"
                     /translation="MEDHLFPAPTKGLTDKRAQLAPATIQAGREFGKQVFAEGARPAK
                     TKQLIAVAVAHVTQCPYCIRGHTKQALKAGASREELMEAIWVAAEMRAGGAYAHSLLA
                     IEAMNESEGRPGGEASTS"
     gene            1228..1419
                     /locus_tag="AC16G0_17210"
     CDS             1228..1419
                     /locus_tag="AC16G0_17210"
                     /inference="COORDINATES: protein motif:HMM:NF024975.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR5101395.1"
                     /translation="MEIVQRTSKGFEVQPKRWIVERTFAWLGRYRRLSKDYERQTETS
                     EAVIQVAMIHLMLSRLCRT"
BASE COUNT          313 a          507 c          541 g          266 t
ORIGIN      
        1 gcggcacgca gacgtcgacc ctggtgaaga cccccaccct cgaggtcatc cgcctgatct
       61 tgcaagcgga caaggaggtc tgccaccacc acgagatgac cggcgagatc atggtccatt
      121 gcctggaggg tctcatcacg gtccagatca atggcgactc gcgcgtcctg gaggccggcc
      181 agatgctcta cctcgccggc cgtgagtcgc acgccatccg cggcctcgac gatgcctcgg
      241 tgctgctgac gatcttgttt cgttgacgga caggccatcc tacggccttt ccggccattt
      301 ctctccggag gttcccatgt ccattctggg gcgatcgccg cgcggtgatc ggcggatgtg
      361 tgccggggga caaccatgag aagtacatcg agtggccagg caacgagtag accagagcat
      421 caggcgggaa cggaggccgg agcaggcgcg gtgcccggcg actgcccgga ggcctcggga
      481 acgctcgcgg ccttggatat tgtcggagag gccagcaggg actcgttccc ggccagtgat
      541 tcgccgggct ggacgaccct ggccgtcggg cctcccgcgc gcaggaacgc tgatcgatcc
      601 gcaccgacct acaccgattt cctctggccg agcatcaacc tcaattgcga tccaggagaa
      661 acccatgtga tggaggatat gatggaggac catctttttc cagcgccaac caagggactg
      721 acggacaagc gggcacagct cgcgcccgcc acgatccaag ctgggcggga gttcggcaag
      781 caggtgtttg ccgagggtgc ccggccggcc aagaccaagc aactcattgc ggtggcggtt
      841 gcacatgtga cccagtgccc gtactgtatt cgcggccaca ccaagcaggc cctgaaggcc
      901 ggcgcatcgc gggaggagct gatggaagcg atctgggtcg ccgccgagat gcgagccggc
      961 ggggcgtatg cacactcact gcttgcaatc gaagcgatga acgaatcgga gggcaggccg
     1021 ggcggcgagg cgtccacatc ctgattgcag aagagcccca gcgccgcctg gcggtggtgg
     1081 tgaccgccgc cagtgtcccg gaccgggacg ggggcaaact cgtcctgaag agcatcgatc
     1141 tccgctaccc ccggttgaag gcggatctgg gccgacgggg cgtaccaggc gatcatcgcc
     1201 tggacacggt ggacctgcct ggccgtattg gagatcgtac agcggacgtc caaagggttc
     1261 gaggtacaac cgaagcggtg gatcgtggag cggacgttcg cctggctggg gcggtatcgt
     1321 cgcttgagca aggactacga gcggcagacc gagacgagcg aggcggtgat ccaggtggcg
     1381 atgattcatc taatgctcag ccgcttatgt cgtacctgat cccgggctac tcgcgcactt
     1441 tctgagagga tgatcccgga tgaaccctcc gtacacctac atcgccgacc tggcccagga
     1501 agcggagccc ccggccgacg gcatcctgag tcgcacactg tacaacgacg aatacctcaa
     1561 ggcggtcatc ttcggcttcg gggtggggca ggagctctcg gagaacacct cgacgatgcc
     1621 cgccacc
//
LOCUS       DBNGZC010001001         7103 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2237895, whole genome shotgun sequence.
ACCESSION   DBNGZC010001001 DBNGZC010000000
VERSION     DBNGZC010001001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2237895"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="AC16G0_17215"
     CDS             complement(<1..880)
                     /locus_tag="AC16G0_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013032296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR5101396.1"
                     /translation="MRTRPGDPYPLGATWDGVGVNFALFSEHAEGVELCLFDGPEGSE
                     EVARIPLREQTDLVWHIYLPEVRPGQRYGYRVHGPYDPARGHRFNPAKLLLDPYAKAI
                     DGAIRWGDALFGYQVSHPDGDLSLDDRDSAASLSKSVVIDPAFTWGEDRPPRIPWNET
                     VIYEVHVKGFTARHPDVPKSLRGTYAGLACPAAIDHLRSLGITAVELLPVHQFVADRH
                     LVERGLTNYWGYNSIGFFAPDVRFARDGILGRQVIEFKTLVKTLHQAGIEVILDVVYN
                     HTGEGSHLGPTISFRGV"
     gene            complement(877..1011)
                     /locus_tag="AC16G0_17220"
     CDS             complement(877..1011)
                     /locus_tag="AC16G0_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101397.1"
                     /translation="MDHAVLPVPGGVQAEAVPHHGQPFCLRLTQPPLAALMLRPEGKR
                     "
     gene            complement(1031..1465)
                     /locus_tag="AC16G0_17225"
     CDS             complement(1031..1465)
                     /locus_tag="AC16G0_17225"
                     /inference="COORDINATES: protein motif:HMM:NF014062.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR5101398.1"
                     /translation="MGWRAPRIFWEYLLLQLPDVFLAGVVLWLLLRWGILSWGWAVGL
                     FGLWVAKDLAMFLCLRNAFGPTRTGFGALVGVHGVAKERLSPRGHVLLQGELWWAETV
                     QPEQVVAPGTPIVVRATRGLTLLVEGEPLSSAEDRRGTQADG"
     gene            complement(1625..1927)
                     /locus_tag="AC16G0_17230"
     CDS             complement(1625..1927)
                     /locus_tag="AC16G0_17230"
                     /inference="COORDINATES: protein motif:HMM:NF024245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HIR5101399.1"
                     /translation="MTATPRDLINLNAASQHELTQLPRIGVDKARRIVHYRAIRKGFR
                     DWEDFAGISGITAEDVEAIRARAWIGPPAQPEPIGLDRGRTGHAEAMVRRPKAKAS"
     gene            complement(2090..3994)
                     /gene="treZ"
                     /locus_tag="AC16G0_17235"
     CDS             complement(2090..3994)
                     /gene="treZ"
                     /locus_tag="AC16G0_17235"
                     /EC_number="3.2.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033942 -
                     4-alpha-D-(1->4)-alpha-D-glucanotrehalose trehalohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HIR5101400.1"
                     /translation="MGARIKATAVGATCLPDARVRFRVWAPRCRSLAVRIVAPRPLGP
                     QEMGRSRGGWFEVTLPSIGPGARYVYVLDGQRERPDPASRALPEGVHGPSEVIDTAAF
                     AWTDSGWRGMPLKNMVLYELHVGSFTPEGTFEAVIPRLEQLRDLGVTAVELMPVASFP
                     GAHNWGYDGVGLFAPQRTYGGPAGLQRLVDACHARGLAVVLDVVYNHLGPEGNYLAEY
                     GPYFTDRYTTPWGPAINYDGDDARGVREFILANALYWVREYHVDGLRLDAIHGIFDGS
                     PVHILRELNQAVQRLAHRLDRSVPVVAESDLNDRRVIDPVRKGGYGLAGQWDDDFHHS
                     LHTLLTGERAGYYQDFGSLEMLAKAYAQGFVYDGQYSSFRGRGHGTPTSDIPPDRFVV
                     FAQNHDQVGNRARGDRLSALVEPRALKLAAATVLLSPYVPLIFMGDEYGERAPFLFFT
                     DFGDPFLQAAVSRGRRDEFAAFGWTGDVPDPQDPATFAVSKLNLSLSEQEPHNRLREF
                     YRALLALRQDYPVLGATGRRNLRARVIEGRTLAVFRRGANAAAFFLLHFAAESRLVRL
                     RVPAGAWRRLMDSAEERFGGPGAKSPAFLPLERGVWAEIEMAGWGACLYLREGSGVTV
                     PVAGGTGILE"
     gene            complement(4278..4703)
                     /locus_tag="AC16G0_17240"
     CDS             complement(4278..4703)
                     /locus_tag="AC16G0_17240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5101401.1"
                     /translation="MPERTILIVDDDAEMRALLVEVLGHEGYRVAEAADGAEALIRLR
                     SECFDGIIIDKNMPGLSGLDLLPGLRMICPDVPVILITAFGDASTYREALEKGALEYL
                     FKPFRLDDLLRVLRGAIPKRPIPTGNGPLASPLGPGGPQ"
     gene            complement(4962..6350)
                     /locus_tag="AC16G0_17245"
     CDS             complement(4962..6350)
                     /locus_tag="AC16G0_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR5101402.1"
                     /translation="MRPEPSRILAVEDDPEMLALLQEHLESEGHRVIAVREGAQALQR
                     LRAEPIDLVLTDLKMPNVDGMEVLRAAREGAPDIPVILITAFGSIETAIQAMRQGAYD
                     YVTKPFALEEISLLVAKALETRHLRAENLRLREELEGRYRFENLLGKSPAMQTVFDLI
                     RRVAGGESNVLITGATGTGKELVARAIHFNSSRAKGPFVPVNCAAIPEGLLESELFGH
                     VKGAFTGAVAAHRGLFLGAAGGTLFLDEIASMPLALQAKVLRAIQEREVRPVGGSEVS
                     PVDLRLVAASNRDLKTLVTAGEFREDLYYRLAVIPVHLPPLRERLEDIPLLAEAFLRK
                     YAVSVGRAIKGFSPEAMAQLLAHPWPGNVRELENVVERAVNLCGHEEISAADLSLDAG
                     VAGTGFSLKGLRPRPTLEELNQAYTAITLEQVNNDKEQAARILGISKRTLYRWLQKPG
                     EDDDKTTTSVTS"
     gene            complement(6434..>7103)
                     /locus_tag="AC16G0_17250"
     CDS             complement(6434..>7103)
                     /locus_tag="AC16G0_17250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5101403.1"
                     /translation="LDAAHPLARHMAIILQQIERIGKIIRQLLEYTRPRRSQIRPTPA
                     GPIVTRTLELMSPLARQRQVRLQADVPADLPPLAADPDQLQQVLLNLLTNALHATSAG
                     GQVTVSARRRETLPPDARPHLERGRAEDPCLWLTVEDTGSGMTPEQMERIFEPFFSTK
                     GGGLGTGLGMSIVEDILRVHHGAIRVSSAKDAGTSVRLLWPIADHGREASPQQGTTPP
                     EDAP"
BASE COUNT         1220 a         2381 c         2299 g         1203 t
ORIGIN      
        1 ccacgccccg gaaagagatc gtgggtccca ggtgactccc ttccccggtg tgattgtaca
       61 caacgtccag gatcacctcg attcccgcct ggtgcagggt cttcaccagc gtcttgaact
      121 cgatcacctg ccggcccaag atgccatccc tggcaaaccg cacgtccggc gcgaagaacc
      181 cgatggaatt gtagccccag taattggtca acccgcgctc caccagatgc cggtcggcca
      241 cgaattgatg gaccggcagg agttccaccg cggtgatccc cagggaccgg aggtggtcga
      301 tggcggccgg gcacgccagc ccggcgtagg tcccccggag agatttggga acatccgggt
      361 ggcgggcagt gaagcccttg acgtgcacct cgtagatcac ggtttcgttc caggggatcc
      421 gcggcggccg gtcctcaccc caggtgaagg ccgggtcgat caccacgctc ttggaaagac
      481 tggcggcgct gtcccgatcg tccaggctga ggtcaccatc cgggtggctc acttgatagc
      541 caaagagggc gtcgccccag cggatggccc cgtcgatcgc cttggcatag gggtccagga
      601 gcagcttcgc cgggttgaac cgatggccac gggcggggtc gtagggtccg tgaacccggt
      661 agccgtagcg ctgccccggc cggacctcgg gcagatagat gtgccagacc aggtcggttt
      721 gctcccgcaa cggaatccgg gcgacttcct cgctcccctc cggtccgtcg aacaggcaca
      781 gctccacccc ctcggcgtgc tcggagaaga gggcgaagtt gaccccgacg ccgtcccagg
      841 ttgcgcccag gggatatggg tcgccgggtc gtgttctcat cgctttcctt ccggccgaag
      901 catcagcgca gccagcggag gctgcgtgag gcgcaggcag aacggctggc cgtggtgcgg
      961 gacggcctcg gcctggacgc cgcccgggac cggcaggacg gcatgatcca tccggacgag
     1021 ggcgccccat ttacccgtca gcctgtgttc ctctcctatc ttcggccgat gacagtggtt
     1081 ccccctcgac caggagtgtg aggccacggg tggcccggac gacaatgggc gttccgggag
     1141 cgacgacctg ctcaggttga acggtctcgg cccaccacaa ttcgccctgc aggaggacat
     1201 gccccctggg agagagccgt tcctttgcga caccgtggac cccgacgagg gcgccgaacc
     1261 ctgtgcgcgt gggcccgaag gcattccgca ggcagaggaa catcgccagg tccttggcca
     1321 cccagagacc gaacagaccg acggcccagc cccaggagag tatcccccac ctgaggagca
     1381 gccagaggac cacacccgcc aggaaaacgt ccgggagttg aaggagcagg tactcccaga
     1441 atatccgggg tgcccgccac cccaccgcca ctcctcctgt cctcccccca tggtctcgct
     1501 cttcctcact gcttcctgcc gctggggacc tccctcgtat tctcggccgg tcgcacggcc
     1561 tgttcgatcg gcgccagcgg ggccgccatc tccttccgaa tcgcctccgg ggtgctctac
     1621 acgctcacga cgcctttgcc ttgggccggc gcaccatggc ctcagcgtgc cccgtccggc
     1681 cgcggtccag ccctatcggt tccggctgtg cgggcggtcc gatccatgcc cgggcccgaa
     1741 tcgcttccac gtcctcggca gtgatcccgg agatcccggc aaaatcctcc caatcgcgga
     1801 accctttgcg tatggctcgg taatgcacga tgcgccgggc cttgtccacg ccgatccggg
     1861 gaagttgagt cagttcgtgc tggctggcag cgttgagatt gatcaggtcc ctcggcgtcg
     1921 cagtcatgga cgacctccgc cttctccccg tcctccgggg gtaacgaaaa tgggtctgtg
     1981 acgatcagtc ccctgtgtcc aagaggggac cgggggagtg aagagcacga agtagaatcc
     2041 cgccacgacg gcaagcttca tggctgaacc ttctgcattt ggggcacctc tattccagta
     2101 ttccagtgcc gcctgccacg ggaaccgtca caccagagcc ctcacggaga tagaggcaag
     2161 ccccccaccc cgccatttcg atctctgccc aaacgccccg ctctaggggg aggaacgctg
     2221 ggctcttcgc ccctgggccg ccaaatcgct cttcggcgct gtccatcagg cggcgccaag
     2281 ctccagccgg aacccgcagt cggaccaaac ggctttcggc cgcaaaatgc aaaagaaaaa
     2341 acgccgccgc atttgctcca cgtcggaaca ccgccagcgt ccggccctcg atcactcggg
     2401 ctcggagatt ccgcctgccc gtggcaccga ggaccgggta gtcttgccgc aaggcaagca
     2461 aggctcggta gaactcccgg agcctgttgt gcggctcctg ttcggacagg ctcaagttca
     2521 gcttggagac ggcgaaggtc gcggggtcct gggggtccgg gacatccccc gtccacccga
     2581 acgcggcgaa ttcatctcgt cggcctcggc tgaccgccgc ttgtaggaaa gggtcgccga
     2641 agtcggtgaa aaagagaaag ggcgcccgct ccccgtactc atcgcccatg aagatcagcg
     2701 gaacgtatgg ggagagcagg acggtcgcgg ctgcgagctt cagcgccctc ggctccacga
     2761 gggcggataa ccggtctccc ctggctcgat tgccgacctg atcgtggttc tgggcgaata
     2821 ccacgaacct gtccggtggg atgtcggagg ttggcgttcc atgcccgcga ccgcggaacg
     2881 aggaatactg gccgtcgtac acgaatcctt gagcatatgc cttcgccaac atttccaggg
     2941 agccgaagtc ctggtagtac ccggcgcgct cccccgtgag gagggtgtgc aggctatggt
     3001 ggaagtcgtc gtcccactgt cccgccagtc catacccacc cttgcgcacc ggatcgataa
     3061 cccgacggtc gttgagatcg ctctcggcca ccacgggcac actgcggtcc aggcgatggg
     3121 ccagccgctg gaccgcttgg ttcagctcgc gcaggatgtg cacggggctc ccgtcaaaga
     3181 tgccgtggat ggcgtccagg cgtagaccgt cgacgtggta ctcgcgaacc cagtacaagg
     3241 cattggccag gatgaactcc cgcacgccgc gggcgtcatc tccgtcatag ttgatggcgg
     3301 gtccccaagg ggtcgtgtac cggtcggtga agtacggacc atactcggcg aggtagtttc
     3361 cttccggccc gaggtggttg tacaccacat ccagcacgac ggccagaccg cgggcgtgac
     3421 aggcgtccac cagccgctgg agcccggccg gaccgccgta ggtgcgttgc ggggcgaaca
     3481 gacccacgcc gtcatagccc cagttgtggg cgccggggaa gctcgccact ggcatcagct
     3541 cgacggcggt gacccccagg tcccggagtt gttccagccg agggatgacg gcctcgaagg
     3601 taccctcggg tgtgaagctg ccaacgtgca gttcgtaaag gaccatgttc ttcaacggca
     3661 tgccgcgcca gcccgaatcc gtccaggcga aggccgcggt gtctatgacc tcggagggtc
     3721 cgtgaacgcc ttcgggcaga gcccgcgaag cggggtcggg ccgctcgcgc tgcccatcca
     3781 ggacatagac ataccgggca cccggaccga tcgacggtag cgtgacctca aaccaccccc
     3841 ctcttgaccg gcccatctcc tgggggccga gaggcctggg agccacgatc cgcacggcca
     3901 ggctgcgaca gcggggggcc cagacccgga agcgcacacg agcgtccggc agacacgtgg
     3961 cccccacagc ggttgccttg atccttgctc ccatgcgtcc ggcacccccg gaaaggggtg
     4021 gtcccaccgc cagcgtatct gccatcatgc gccggccacg acgccctctg ccaacgcgta
     4081 tgccggcatc caggtcgggc gccggctgaa tcggcggacc gtctgcacga tcggctcggg
     4141 atccccccag tcgctccagt acacgttccg gctagccttg ccgccagtcg tgtcccttcc
     4201 ccctcccttc gtcagcgcct cgaattaccg gctccgaacg ctgtggggtc ccatcccagt
     4261 agaatggcca ggccaactca ttggggtccg ccgggcccga ggggagacgc cagaggccca
     4321 ttgccggtcg ggatgggtcg ttttgggatg gcccccctca ggacccggag caagtcgtcc
     4381 aagcggaagg gcttgaagag gtactcaagg gctcccttct ccaatgcctc tcgatacgtg
     4441 gaggcatctc cgaaggcggt gattaggatg acgggaacgt caggacagat catccgcaat
     4501 cccggcaaga gatccagacc gctcaggccg ggcatattct tatcgatgat gatgccatcg
     4561 aaacactcgg atcgaaggcg aatcaatgcc tcggctccat cggcggcctc tgccacgcga
     4621 tatccctcat gacccaacac ctctactaag agggcccgca tctccgcatc atcgtcaacg
     4681 atcaggattg tgcgttccgg catgtcacat tccccccttg ggactccgag tccctttcca
     4741 ccgcgatccc tcctggtgag ggatggcaag ttcgatgccc gattttcggg ctctacgaca
     4801 tatctatggg tgacaccaaa gcatgagaaa gacaggcgga agctcccgga gcgggaggcc
     4861 atggtgcaga agcgtgatca gaggtgaagc gacatattgg cgagaaggcg gccgtgctgg
     4921 ggcaggctgt cggggggaaa gcgacgtagg aaaagcgcgt gtcaggatgt cacggaagtt
     4981 gtcgtcttgt catcatcttc tccgggcttc tgcagccagc ggtagagcgt tcgcttgctg
     5041 atccccaaga tcctggcggc ctgctccttg tcgttattca cctgctctag tgtgatggcg
     5101 gtataggcct gattaagctc ctccagggtt ggccgcggcc tcagcccctt caggctgaaa
     5161 ccggtcccgg cgacgcccgc atccaggctc agatccgccg ccgagatctc ctcgtggccg
     5221 cagaggttca ccgcgcgctc taccacgttc tccagctcac ggacgttccc tggccagggg
     5281 tgggccagaa gctgcgccat ggcctcgggg gaaaagccct tgatggctcg cccaacggaa
     5341 accgcatact tccgcagaaa ggcctcggcc aggaggggga tgtcctccag acgctcgcgc
     5401 aggggcggga ggtggacggg gatgaccgcc agccggtagt acaagtcctc gcgaaactcc
     5461 ccggctgtca ccagggtctt cagatcacgg ttgctggccg ccaccagccg taggtccaca
     5521 gggctcacct cgcttccgcc caccggacgc acctcgcgct cctgaatcgc gcgcaggacc
     5581 ttggcctgga gggccaaggg catgctcgcg atctcatcca ggaacagcgt gccccccgcg
     5641 gcccccagga acagaccgcg atgcgcagcc acggccccgg tgaaggcgcc cttcacgtga
     5701 ccaaagagtt cgctctccag gaggccctcg ggtattgctg cgcagttgac cgggacgaag
     5761 ggtcccttgg cccgcgagga gttgaagtgg atggctcgcg ccacgagctc cttcccggtc
     5821 ccggtggcgc cagtgatgag gacgttgctc tcgccgcccg ccacgcgccg gatgaggtcg
     5881 aagacggtct gcatggctgg gctttttccc aggaggttct cgaagcggta gcggccttcc
     5941 agctcctctc gcaggcgcag gttctcggcg cgcagatggc gggtctccaa cgccttggcc
     6001 accagcagac tgatctcctc cagggcgaag ggcttggtca cgtagtcata ggccccttgg
     6061 cgcatggcct ggatggcggt ctcgatgctg ccgaaggcag tgatcaggat gaccgggatg
     6121 tccggcgcgc cctcccgggc ggcccgcagg acctccatcc cgtccacgtt gggcatcttg
     6181 aggtcggtga ggaccaggtc aatgggctcc gcacgcagtc gctggagcgc ctgggcgccc
     6241 tcgcgcaccg cgatgacgcg gtgaccctcg gattccaggt gctcctggag gagggccagc
     6301 atttccgggt cgtcctcgac ggcgaggatt cgagacggtt cgggccgcat ggctcacctg
     6361 tgactctgtg ctgcgtggtt gaccggagga gtcggcgtcg aacctcctcg acccccgatg
     6421 atctcaccgc agctcacggg gcgtcctccg ggggcgtcgt cccctgctga ggggacgctt
     6481 cccttccgtg gtccgcgata ggccagagga ggcgcacgct cgtccccgcg tccttggcgc
     6541 tgctcacccg gatcgcgcca tggtgcacgc ggaggatgtc ctcgacgatg gacatgccca
     6601 gaccggtgcc cagaccgccg cccttggtcg agaagaacgg ctcgaagatc cgctccatct
     6661 gctcgggcgt catcccgctg ccggtatcct ctaccgtgag ccagaggcag gggtcctcgg
     6721 cccgaccgcg ctccagatgc gggcgggcat ccgggggcag ggtctcccgc cgccgggccg
     6781 agaccgtgac ctgcccgcct gcggacgtgg cgtggagggc gttggtcagc agatttagga
     6841 gaacctgctg gagctggtca ggatccgcgg ccaggggagg aaggtcggcg ggcacatcgg
     6901 cctggagcct gacctgccgc tgacgcgcca agggagacat gagctccagg gtccgggtga
     6961 cgatggggcc cgcgggcgtg gggcggatct gggagcggcg aggccgggtg tactccagga
     7021 gctgccggat gatcttgccg atgcgctcga tctgctggag gatgatcgcc atgtggcgcg
     7081 ccagcgggtg agcggcgtcc agc
//
LOCUS       DBNGZC010001002         7340 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1849132, whole genome shotgun sequence.
ACCESSION   DBNGZC010001002 DBNGZC010000000
VERSION     DBNGZC010001002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7340)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7340)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7340
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1849132"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="AC16G0_17255"
     CDS             <1..207
                     /locus_tag="AC16G0_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008942888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIR5101404.1"
                     /translation="RVFDIVWVITKGEPYRSTEVFSIYAYKQAFVYLNFDQGSAAAIV
                     GAIIIMIVGGVLYWGLSRLLEVSR"
     gene            211..1014
                     /locus_tag="AC16G0_17260"
     CDS             211..1014
                     /locus_tag="AC16G0_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5101405.1"
                     /translation="MLAPGWKKAFFGLTLAAVCLLIAFPILWITLTSLRPASEVFYVH
                     RGTHFTLRNFLEVLAKPEIKHSFFNSGLIATLATILSIQVTITSGYMLSRFRGKMANT
                     WFGLIYVFRAIPYIAWVLPLYVVEQNLGILDSYWGLLLPHMSVHICFFSWLMKGFFDG
                     IDPDMEFAAQIDGCSRWGAFFKIALPQAVPGVAALAILTWLWTWNEFLFALILTGSKT
                     PMLTVTMAQFVHELGIEWNLMSATAVMALLPAIIVVLFAQEYVVKGLRV"
     gene            1116..1868
                     /locus_tag="AC16G0_17265"
     CDS             1116..1868
                     /locus_tag="AC16G0_17265"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5101406.1"
                     /translation="MKLEGQVALVTGGSRSIGRAIALGLAREGADLAVNYVQHADAAQ
                     GAVREIQALGRRAIAVRADTSRQAEVRAMVAEVTQALGPIDILVNSAGVQKRVFFLDL
                     YEEDWDWMLDVNLKGYFLVGQAVAAGMKARGKGKIVNVSSEAGGFPAPRMTAYCVSKA
                     GVAMLTKCMALELAAHGIRVNALAPGLTRTDINRKDLEDETFLKARLQRIPLGRVMNP
                     EDLVGAAVFLASTDSDSMTGQTLQVDGGRGIA"
     gene            2007..2765
                     /locus_tag="AC16G0_17270"
     CDS             2007..2765
                     /locus_tag="AC16G0_17270"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5101407.1"
                     /translation="MGKVALVTGAAAGIGYAIATRLALAGYDIAIADVDAAGAERAAH
                     GLHRDGRRATASVCDVGDRLAVHRMVESISRTLGDVDLLVNNAGIARLGPLASFSEAD
                     WRELFRVNVDGVFFCCQAVLPGMLERGRGNIVNISSWNGKLGAPNFGAYSATKAAVIS
                     LTQALAREVAARGIRVNAVCPGIVAGTPMRKEVERQGQAFGLPPSSERAKVVPMGRLA
                     TPEDIAGAVAFLASDEAAYMTGQAINVTGGLWLH"
     gene            2817..3938
                     /locus_tag="AC16G0_17275"
     CDS             2817..3938
                     /locus_tag="AC16G0_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101408.1"
                     /translation="MQKTRTLEAGLALVCERVGSTLEQVAREDIYPHAAPSATGVWEG
                     KREGMWTSGFWAGLLWQKAALTGRPEDADTARVWTLRLAPHLDHKTHDIGFVFSTSAV
                     LGWEIGRVAACRDLGLQAADRLADMFNPAAGVIPVGSQAEIAAGLDDVTIDCMANLPL
                     LWWAWQATGEERFRQIAVSHADRTAGWHVKQDGRIIQSTHFDPVTGTMTKQDTHQGSG
                     TEGCWSRGQAWAVYGFAAAYRATGEEHFLEVADRAATYYLCRAGSDVVPMYCFDDPGG
                     PDVPRDSSAAAITCAGLWILATHAQDPIRRQRSRDRAETLLRALVTDHLTPQGEADRR
                     PRGMLPHGCYSYRAGWDTDHELIWGDYFLLQALRAWAQR"
     gene            3993..5516
                     /locus_tag="AC16G0_17280"
     CDS             3993..5516
                     /locus_tag="AC16G0_17280"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HIR5101409.1"
                     /translation="MAPEIVTASQAAQLIPDGATVVLSGCLSVLEPDAALRGIETRFL
                     EEGHPRDLTVIHPVMVASAKGTGINRFAHNGLMRRVIGGSFSIFPDYEITGMIKQDRV
                     EAYNLSIGTIFHWLRESGAGRPGLLTGVGLHTYLDPRVEGGRANAAAREPLCQVVDFA
                     GREWLFYPALKVDVAIIRGTSADSHGNISIEHEPASLGIYYLALAAKHSGGKVIAQVK
                     QRVEAGTIHPRLTVVPGSLVDAIVVDPTQPQTAMGEHPEFSGEARIPLHADPVPLTVN
                     SVIARRAMQEVQDGQLVNLGYGIPALIPALGFPTRLHERVAFSIEHGPVGGLPEGMEA
                     FGATANPDILMDSTCVFDLYDGGLLDVTLLGLAQVDREGNVNVSKFAHMAPGSGGFCN
                     ICHRTKKVVFCGTFTAGGLQAEVVNGEVVIRKEGKLRKFVRKVEQITLSGRAAREKGQ
                     EILYVTERGVLRLAKDGLELIEVAPGIDPATHILPHMEFSISIPAGVRSMDRALFHA"
     gene            5835..6743
                     /locus_tag="AC16G0_17285"
     CDS             5835..6743
                     /locus_tag="AC16G0_17285"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013608620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR5101410.1"
                     /translation="MRPEALTRVDPAKVLAEKNRQKKEAMLQYLAQKFRIDVFDILHD
                     KGTGHWGGASSAAELLTALYFDGMNVRPEEPRWPDRDRLVLSKGHASAILYTVLAYRK
                     FFPVEELDTFRQLNSRLQGHPCLNETPGVDMSTGALGHGLSVSLGMALAARVQQKKYW
                     TYVIMGEGCLDEGQTWEGIMAAAKFKPERLVAMIDYNGFQLDGPSNEIMPLAPLFEKF
                     HAFGWNVSPREWNGHSIPEILQSFEWVRQQKEWPVALIYKTQKGRGISFTQDTHKFHG
                     AVIDDQSYAKGRPELMRTLAELEVAL"
     gene            6740..>7340
                     /locus_tag="AC16G0_17290"
     CDS             6740..>7340
                     /locus_tag="AC16G0_17290"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR5101411.1"
                     /translation="MKTASKAIAMRDAFGQALMELAGEMPEMVVLDADVSSSTKTAGF
                     GTAHPDRFFNVGVAEANMADIAAGLATCGLRPVISTFSLFLTLKCADQIRNTICYNNL
                     PVVLAGGYGGLSDSYDGASHQAILDIAMMRALPNMVVIVPADAVEIRQALRQALRRNG
                     PTFIRICRNETPVLFEDAAPLEIGKIRKLREGSDVTIGVC"
BASE COUNT         1297 a         2237 c         2541 g         1265 t
ORIGIN      
        1 cgggtcttcg acattgtctg ggtcatcacc aaaggggagc cgtatcgctc gacggaggtc
       61 ttcagcatct acgcctacaa gcaggccttc gtgtacctca acttcgacca aggctcggcc
      121 gcggcgatcg tcggggcgat catcatcatg atcgtcggcg gcgtcctcta ctggggtctg
      181 agccgcctgc tggaggtctc gaggtaaact atgctggcgc cgggatggaa gaaggccttc
      241 ttcgggctca ccctggccgc cgtctgcctg ctcattgcct ttcccatcct ctggatcacc
      301 ctgacctcgc tgcgtcccgc ctcggaggtt ttttacgtgc atcggggaac gcactttacc
      361 ttgcggaact tcctggaggt cctggccaag ccggaaatca agcactcttt cttcaacagc
      421 ggcctgatcg ccacgctggc gacgatcctc tccatccagg tcaccatcac cagcggctac
      481 atgctttcgc gcttccgtgg gaagatggcg aacacgtggt ttggcttgat ctacgtgttt
      541 cgggccatac cctacatcgc gtgggtgttg cccctctacg tggtggaaca gaatctcgga
      601 atcctggatt cgtactgggg gctcctcctg ccccacatga gcgtgcacat ctgcttcttc
      661 tcttggctca tgaaggggtt cttcgacggg atcgacccgg acatggagtt tgcggcccag
      721 atcgacggct gttcgcgctg gggcgccttc ttcaagatcg ccctgcccca ggccgtcccg
      781 ggggtggctg cgctggctat cctcacctgg ctctggacct ggaacgagtt cctgtttgcc
      841 ctgatcctga ccgggagcaa gacccccatg ttgacggtga ccatggccca gtttgtccac
      901 gaactgggga tcgagtggaa cctgatgagc gcgaccgcgg tgatggcgct tctgcccgcc
      961 atcatcgtgg tcctgttcgc tcaggagtat gtggtcaagg ggctgcgggt atgatgcggc
     1021 cagtgcttgt caagaaaccg ggaaggacgg ctcgcatctg acggccgcct ccctcccctg
     1081 agttgccatc acgctcacac acaggagaga ccaacgtgaa gcttgaaggg caggtggccc
     1141 tcgtcaccgg gggctcgcgc agcatcggac gcgccatcgc gctcggcctc gctcgtgagg
     1201 gggcggattt ggccgtgaat tacgtgcagc acgccgacgc ggcgcagggc gccgtgcgtg
     1261 agatccaggc attgggtcgc cgggccatag ccgtccgcgc cgacacgtcc cggcaggcgg
     1321 aggtccgggc catggtagcg gaggtgacgc aggccctcgg cccgatcgac atcctggtga
     1381 acagtgcggg cgttcagaag cgggtcttct tcctggacct ctacgaggag gactgggatt
     1441 ggatgctgga cgtgaatctg aaggggtatt tcctggtggg acaggcggtg gccgccggca
     1501 tgaaggccag gggcaagggc aagatcgtca acgtgtcctc ggaggcaggc gggtttccgg
     1561 ccccccgaat gacagcctac tgtgtgtcca aggccggggt ggccatgctc accaagtgca
     1621 tggcgctgga gcttgcggcc catgggatcc gggtcaatgc cctggcaccg ggcctcacgc
     1681 gcaccgacat caatcggaag gacctggagg acgagacctt tctgaaggcg cggctccagc
     1741 ggattcccct cggtcgggtg atgaaccccg aggatctggt gggggcggcc gtctttctgg
     1801 cctcgacgga ttccgacagc atgacggggc agactcttca agtggacggg ggaaggggga
     1861 ttgcctgaga cggggggcgg agagcagaga gccgcgagca gagagggcgg agggcacgga
     1921 ggacgggtca gggagcgcaa gagtacagag cacagggtac ggggtgcagg acagacggca
     1981 cgggcagcag acagggggaa ggacacatgg gaaaggtggc gctggtcacg ggggcggctg
     2041 cgggcatcgg gtatgccatc gccacgcggt tggcactggc gggatacgat atcgccattg
     2101 ccgacgttga cgcggcgggg gccgagcggg cggcccatgg gctgcatagg gatgggcggc
     2161 gggccacggc cagcgtctgc gatgtgggcg accgtttggc cgtccaccgg atggtcgaga
     2221 gcatttcccg gactctgggc gacgtcgacc tgctggtcaa taacgccggg atcgcgcgcc
     2281 tgggcccctt ggcgagtttt tccgaggcgg actggcgtga gcttttccgg gtaaacgtgg
     2341 acggcgtctt cttctgttgc caggccgtgc ttccagggat gctggagcga gggcggggca
     2401 atatcgtgaa catctcctcg tggaatggga agctcggggc gccgaacttc ggggcctaca
     2461 gcgcgaccaa ggcagccgtg atctcactca cgcaggcttt ggcgcgggag gtggcggccc
     2521 gcgggatccg ggtgaatgcc gtctgtccgg ggatcgtggc cggcaccccc atgcgaaaag
     2581 aggtggagcg ccaggggcaa gccttcgggc tgcccccgtc ctctgaacga gcgaaggtgg
     2641 tccccatggg ccgtctggcc acgcccgagg acatcgcagg cgcggtggca ttccttgcct
     2701 cggatgaagc cgcctacatg acgggccagg cgatcaacgt gaccggaggg ctgtggctgc
     2761 actgatggga tttcggatgg cggatctcga atctcggatg tggggaagcg aaaacaatgc
     2821 agaagacgcg gacgctggaa gcagggctgg ccctggtatg cgagcgggtc ggcagcacgc
     2881 tggagcaggt cgcgcgtgag gacatctacc cgcacgccgc gccctcggcc accggcgtgt
     2941 gggaggggaa gcgggaaggg atgtggacga gcggcttttg ggccgggctc ctgtggcaga
     3001 aggccgcgct aaccggcagg ccggaagacg cagacacggc cagggtgtgg accctgcgcc
     3061 tcgcgcccca tctggatcac aagacccacg acatcggatt tgtgttctcg acctccgccg
     3121 tcttggggtg ggagatcggc cgggtcgcgg cctgccggga cctggggctg caggcggcag
     3181 acagactcgc ggacatgttc aaccctgccg ccggggtcat ccctgtcggc tcccaggcgg
     3241 agatcgcggc tggcctggac gacgtgacga tcgactgcat ggcgaaccta ccgctgctct
     3301 ggtgggcatg gcaggcgacg ggggaggagc gcttccgcca gatcgccgtc tctcacgcgg
     3361 atcggaccgc cggctggcac gtgaagcagg atggacggat catccagtcc acccacttcg
     3421 acccggtcac gggaacgatg acgaagcagg atacgcatca aggctccggg acggaaggat
     3481 gctggtctcg agggcaggcc tgggccgtct acggcttcgc cgccgcgtac cgggcgacgg
     3541 gcgaggaaca cttcctggag gtagccgacc gtgcggccac atattatttg tgccgggccg
     3601 gaagcgacgt cgttcccatg tattgcttcg acgatcccgg cgggccggat gtcccccggg
     3661 attcgtcggc ggcggccatc acctgtgcgg gcctgtggat tctcgcaaca catgcgcagg
     3721 acccgatccg gcggcagcga tcccgggacc gcgccgagac gctgctccgg gccctggtga
     3781 cggaccacct cacgccgcag ggagaggccg accgtcggcc gcgggggatg ctgccccatg
     3841 gctgctacag ctatcgggcg ggttgggata ccgaccacga gctgatctgg ggcgactatt
     3901 tcctgctgca ggcgttgcgg gcgtgggccc aaagataagg caataagtgc ctggccccag
     3961 agcgggaagg gcgggggtga aggaagggga gaatggcacc cgagatcgtc acggcctccc
     4021 aagcggcaca actgatcccg gacggtgcga ccgtggtcct gagcggctgc ctctcggtgt
     4081 tggagccgga tgcggcgctt cgagggatcg agacgcggtt cctcgaggaa gggcatcctc
     4141 gggatctcac ggtgattcat ccggtgatgg tggcgagcgc caagggcacc ggcatcaacc
     4201 gtttcgcgca caatggcctg atgcggcggg tgatcggcgg gtcattcagc atcttcccgg
     4261 attacgagat caccgggatg atcaagcaag accgggtcga ggcttacaac ctgtccattg
     4321 ggacgatctt ccactggctg cgggagagcg gggcggggag gccgggactc ctcaccgggg
     4381 tggggcttca cacctacctc gatccccggg tcgaaggggg ccgagcaaac gccgcggcga
     4441 gggagccatt gtgccaggtg gtggacttcg cggggcgaga gtggctcttt tatccggccc
     4501 tcaaggtgga cgtggccatc atccggggga cttcggccga ctcgcacgga aatatctcca
     4561 tcgagcatga gccggcatcc ctggggatct actacctggc gctcgcggcg aagcactccg
     4621 gcggaaaggt catcgcccag gtgaagcagc gggtggaggc tggaacgatc cacccccgcc
     4681 tcaccgtcgt ccccggaagc ctggtggacg caatcgtggt ggacccgacc cagccccaaa
     4741 ccgccatggg tgagcatccg gaattctccg gtgaggcgcg catccctttg catgccgatc
     4801 ccgttccgct gacggtcaac tcggttatcg ccagacgcgc catgcaggag gtccaggatg
     4861 gccaacttgt caacctgggc tacgggattc cggctctgat tcctgccctg ggcttcccga
     4921 cgcggctgca cgagcgcgtc gccttcagca tcgagcacgg gccggtgggc ggcctccccg
     4981 aggggatgga ggcctttggc gccacggcca atcccgacat cctgatggac tccacatgcg
     5041 tgtttgatct gtacgacggg ggactcttgg acgtgaccct gctggggctc gcccaggtag
     5101 atcgggaggg gaatgtcaac gtcagcaagt tcgcccacat ggcaccgggc agcggcggct
     5161 tctgcaacat ctgtcaccga acgaagaagg tcgtgttctg cggaacattc acggcgggcg
     5221 gtctccaggc cgaggtcgtg aatggcgagg tcgtcatccg gaaggaaggc aagctcagga
     5281 agttcgtccg aaaagtcgag cagatcaccc tgtccggcag ggccgcccgc gaaaagggac
     5341 aggagatcct gtacgtcacc gagcggggcg tcctgcgtct ggctaaggac ggcctggagc
     5401 tcatcgaggt ggcccccggc atcgatcccg ccacccacat ccttccgcac atggagttct
     5461 cgatcagcat cccggcagga gttcgcagca tggaccgcgc actgtttcac gcatgaccca
     5521 ccggtccgcc atggcgtgct tggcgccttg gcggttgagt caagatccgt ttcatagact
     5581 tcgctttgcg ctcccccgca ttgtcagcaa ttcctatcca gccgatcccg cggattgacc
     5641 ggaggagcgg gaagggggat agtgggagtc tcgcaaggcc gtcggtggga cgacttggcc
     5701 cggacggcgc attggtgttc cggggcgcga cgaaggattg cggggagacg ggcggcggtc
     5761 gtccgcgaat tccccggcat gtgggtcagc ccggagaccg accagaggag gtcgcaccgc
     5821 agaaaggggg tgccatgaga ccagaggcat tgacacgagt ggatccggcc aaggttctgg
     5881 cggagaagaa ccgccagaaa aaggaggcca tgctgcagta cctggcccag aaattccgga
     5941 tcgatgtttt cgacatcctg catgacaagg gcacgggcca ctggggcggc gcgtcctcgg
     6001 cggcggagct tctaaccgcg ctctatttcg atgggatgaa cgtccggccg gaggagccac
     6061 gctggccgga tcgcgatcgg cttgttctta gcaaggggca tgcctccgcc atcctctaca
     6121 cggtgctcgc ctaccgcaag ttcttcccgg tcgaagagct ggacaccttt cgccagctca
     6181 acagccggct ccagggtcac ccgtgcttga acgaaacgcc gggggtggat atgtccaccg
     6241 gcgccctggg ccacggcctg tccgtgtccc tgggcatggc gctggccgcg agggtccagc
     6301 agaagaaata ctggacctac gtgatcatgg gcgaggggtg tctggacgag ggacagacct
     6361 gggagggcat catggccgcg gccaagttca agcccgagcg gctggttgcc atgatcgact
     6421 acaacgggtt ccagctcgac gggcccagca acgagattat gccgctggcc cccctgttcg
     6481 agaagtttca cgccttcggc tggaacgtgt cacccaggga gtggaacggc cacagcatac
     6541 cggagatcct gcaatccttc gagtgggtgc ggcagcaaaa ggaatggccg gtcgcgctca
     6601 tctacaagac gcaaaagggt cggggcatct ccttcaccca ggacacccac aagttccacg
     6661 gggccgtgat cgacgatcaa agctacgcca aagggcggcc cgaactcatg cggactctcg
     6721 cagaactgga ggtggccctg tgaagactgc cagcaaagcc attgcgatgc gagacgcctt
     6781 cggtcaggcc ctcatggagc tggccggcga gatgcccgag atggtcgtcc tggacgccga
     6841 cgtgtcctcc agcaccaaga ccgccggctt cggcacggcc cacccggacc ggttcttcaa
     6901 cgtcggcgtg gccgaggcca acatggcgga catcgccgcc ggccttgcca cctgcggcct
     6961 gcgccccgtg atcagcacct tctccctgtt cctgaccctg aagtgcgcgg accagatccg
     7021 caatacgatc tgctacaaca acctgcccgt ggtgttggcc ggcgggtacg gcggcctctc
     7081 ggattcctat gacggggcga gccaccaggc gatcctggat atcgccatga tgcgcgccct
     7141 gcccaacatg gtggtgatcg tcccggccga cgccgtggag attcggcagg cgctgcgcca
     7201 ggccctccgg cgcaacgggc cgaccttcat ccggatctgc cgcaacgaga cgcccgtcct
     7261 gttcgaggac gcggcgcccc tggaaatcgg gaagattcgc aagttgcggg aaggatcgga
     7321 cgtcaccatc ggcgtgtgcg
//
LOCUS       DBNGZC010001003         1950 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_281480, whole genome shotgun sequence.
ACCESSION   DBNGZC010001003 DBNGZC010000000
VERSION     DBNGZC010001003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_281480"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="AC16G0_17295"
     CDS             <1..1268
                     /locus_tag="AC16G0_17295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HIR5101412.1"
                     /translation="WIEHHEDDLAAGLTRLVSVPSIVGSEGPCQQVVAELMKAVCDVV
                     DIWEPDASWLEKHPAYFLRGVSFSGRPNVVGVLRGRGGGRSIILNAHVDVVDPGPADA
                     WKYSPWSGALSGGKLYGRGSVDDKAGLAVIIFVARCIRSLGLSLQGDVILQSTVDEEW
                     GGGGTLATIQRGYRADAAIVFEPTTLEICPAARGGQAFRVTVPGKGAHPFRSYEGVSA
                     LEKAIPLLAALKRLEGERQGRFRTPLFERYPIFLPIVIGKISADVIPSKVPERCVFDG
                     LMGYAPDEAYLAARQELEACVAGAAGTDPWLKEHSPVVEWLGLNKEGAQTPVDHPLVT
                     LMGESFVRVMGGRPSIVGFPAGCDLPHLRRHAGIPSLVFGPGNPVIAHGSNEHVTLEE
                     VVNAAKILAVALLRWCGGDESSAVLPQTM"
     gene            1333..>1950
                     /locus_tag="AC16G0_17300"
     CDS             1333..>1950
                     /locus_tag="AC16G0_17300"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5101413.1"
                     /translation="MGTRNLLLWRVPWIAAVCLALALIGPGLAGVAGAGEKPKLVVWW
                     NKGYYKEEDEGMQKVAEAFAKENNVDLEITFTIQDDLGPKIISALIARRVPDVAFCFF
                     NDFHISPKFAWDGKLTDVSDLVNELKPRYLESHMATGRLFNNAEKKWAYYAVPIEAQA
                     LGSHYWKDLVQEAGFDPDPKKIPMKWNEHWAFWKKVQDNLRKKDPA"
BASE COUNT          357 a          588 c          668 g          337 t
ORIGIN      
        1 agtggattga acaccacgag gacgacctgg ccgcggggct caccaggcta gtctcggtcc
       61 ccagcatcgt cggctctgaa ggaccctgcc agcaggtggt ggcggaattg atgaaggcgg
      121 tgtgtgacgt cgtcgacatc tgggagccgg atgcctcctg gctggagaag cacccggcat
      181 atttcctgcg gggcgtgagc ttcagcgggc gccccaacgt ggtcggcgtg ctccgaggac
      241 gcgggggcgg caggtccatc atcctcaacg cccatgtgga cgtggtggac ccgggtccgg
      301 ccgacgcctg gaagtactcc ccgtggtctg gggccctctc cggcggaaag ctgtacggca
      361 ggggctccgt tgacgacaag gccggtctcg ccgtgatcat cttcgtggcg cggtgtattc
      421 gcagcctcgg cctgtcgctc cagggggacg tcatcctgca gagcacggtt gacgaggagt
      481 gggggggagg cgggacgctg gcgacgatcc agcggggcta tcgggccgat gcggccatcg
      541 tcttcgaacc cacgaccctg gagatttgtc ccgccgccag gggcggccag gccttcaggg
      601 tgaccgtgcc tggcaagggg gcgcatccgt ttcgatccta cgagggggtg agcgccctgg
      661 agaaggccat cccgctgctg gcggctttga agcggctgga aggagaaaga caggggcggt
      721 ttagaacgcc gctcttcgag cgctatccca tctttctgcc gatcgtcatc gggaagatct
      781 cggcggatgt gatcccctcc aaggttccgg agcgctgcgt gttcgatggc ctgatgggct
      841 atgcgcccga tgaagcctat ctggccgccc ggcaggaact ggaagcctgc gtggcggggg
      901 cggccggaac cgatccctgg ctcaaggaac attccccggt ggtcgagtgg ctggggttga
      961 acaaggaagg ggcgcaaacc ccggtggatc atcccctggt cacgctgatg ggagaatcct
     1021 tcgtccgggt catgggtggg cggcccagca tcgtcggatt cccggcaggc tgcgaccttc
     1081 cccatctcag gaggcatgcc ggaattccca gcctggtgtt cggccctggg aatcccgtga
     1141 tcgcccatgg ctcgaatgag cacgtgacgc tcgaggaggt ggtcaacgcg gccaaaattc
     1201 tggcggtcgc cttgctccga tggtgcggcg gcgacgagag ttccgctgtc ctgccgcaga
     1261 ccatgtgaac cgggcggtcc catcgcccgc ggcaatcccc gtttggtccc gatggagaga
     1321 ggaggagaac ccatgggtac acggaatctg ctcctgtggc gcgttccctg gatcgcggcc
     1381 gtctgcctgg cgctggccct catcgggccc ggcctggccg gggtggcggg cgcaggggag
     1441 aagcccaagc tggtcgtctg gtggaacaag ggatactaca aagaagagga cgaaggcatg
     1501 cagaaggtcg cggaggcctt cgccaaggaa aacaatgtcg acctggagat caccttcacc
     1561 atccaggacg acctgggccc caagatcatc agcgcgctca tcgcccgccg agtacccgat
     1621 gttgccttct gcttcttcaa cgacttccac atctcaccca agttcgcgtg ggatgggaag
     1681 ctgaccgatg tctcggacct cgtcaacgaa ctgaagcccc gctacctgga atcccacatg
     1741 gccacggggc ggcttttcaa caatgcggaa aagaaatggg cgtattacgc cgtccccatc
     1801 gaggcgcagg ccctgggcag ccactactgg aaggacctgg tgcaggaagc cgggttcgat
     1861 cccgacccca agaagatccc catgaagtgg aatgagcact gggccttctg gaagaaagta
     1921 caggacaacc tgcggaagaa ggacccggcc
//
LOCUS       DBNGZC010001004         2756 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1457108, whole genome shotgun sequence.
ACCESSION   DBNGZC010001004 DBNGZC010000000
VERSION     DBNGZC010001004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1457108"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="AC16G0_17305"
     CDS             <1..194
                     /locus_tag="AC16G0_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101414.1"
                     /translation="VTPEEIRRVKELGLTPVVGHFIESDWRGKRVLHKLDTIRHDPHR
                     LAAVLRTLVTDAGSRTVVV"
     gene            288..1298
                     /locus_tag="AC16G0_17310"
     CDS             288..1298
                     /locus_tag="AC16G0_17310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR5101415.1"
                     /translation="MRYRTFGNTDLRASEVGFGVWTVGTSMWGIKDEGVGIRLVQQAL
                     DLGITFFDTADVYGDGLGETILAKAFKGRRDRVVYASKFGYDFYTHPGVQPGQRERPQ
                     DWTPAFVRRACEASLARLDTDHIDLYQLHNPRIDTLRKDDLFAELERLKSEGKIRHYG
                     ATLGPAIDRRQIEEGKCCVAERRMITQIIYNMLEQQIGRPLIPEAKQHGVGLMVRVPH
                     SSGLLEGKYTQETTFGTDDHRFFRVNTDERKKAWLDVGLKKVGKLSFLTEGKGRTIAQ
                     AAIRWILAEPCFACVLPNIYNEGQLHEFAGAADVPDLTAEEVRQVNDLYERNFDLEPA
                     AV"
     gene            1406..>2756
                     /gene="glmS"
                     /locus_tag="AC16G0_17315"
     CDS             1406..>2756
                     /gene="glmS"
                     /locus_tag="AC16G0_17315"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 -
                     L-glutamine:D-fructose-6-phosphate transaminase
                     (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR5101416.1"
                     /translation="MCGIVGYIGDKKVVPILLDGLKRLEYRGYDSAGLAVIEQGRMVV
                     HRSVGRIKELENAVWGSNLIGTIGLGHTRWATHGRPTEENAHPHTDCTGDVVVVHNGI
                     IETYLALKEKLLAEGHFFRSETDTEVIAHLIEKYLKEDRDIEVATRRALNDVTGAYAI
                     GVLWKGDPGRIVGAKCGSPLVVGLGEGDFFFASDIPAILNHTRNVLFLDDEEIVVLTK
                     DGVHITTLAGEEVQRSVEKVLWSPILAEKGGYKHFMLKEIYEQPRAVRDTIRGRFSEE
                     SSQIFFEGMEALDEVLPEIQKVTLIACGTAWHAGLVGKFLIEELCRVPVEVDYSSEFR
                     YRDPVVESRTLVMAVSQSGETLDTLAGIKEARQRGCPTFAVCNVVGSSITREVDGVLY
                     THAGPEIGVAATKTFTAQIAALYLFALYFGRKRGIVDAARMRELLADLVRLPQQIEQV
                     "
BASE COUNT          537 a          819 c          952 g          448 t
ORIGIN      
        1 gggtcacgcc cgaggagatc cggcgagtga aggaactggg cctcaccccg gtcgtgggac
       61 acttcatcga gagcgactgg cgggggaaac gggtattgca caagctggac acgatccgcc
      121 acgatccgca cagactggcg gctgtgctgc ggacgttggt gacggatgcc gggtcccgga
      181 cggttgtggt gtgatgacgg gtagatacag gaccgataac gacagggaca acgagagcga
      241 gcagaattac ctgccagaga tccctgtcgg tcatcaaggg gggagccatg aggtaccgca
      301 ccttcggtaa cacggatctc cgggcgtcgg aggttggctt cggcgtctgg acggtcggca
      361 ccagcatgtg gggaatcaag gacgagggcg ttggcatccg gctggtgcaa caagccctcg
      421 acctggggat cacgttcttc gacacggccg acgtgtatgg cgatggcctg ggggagacca
      481 tcctcgccaa agccttcaaa ggcaggcgtg atcgcgtggt gtatgcctca aagtttggct
      541 atgacttcta tactcatccg ggggtgcagc cgggccagcg ggagcgcccg caggactgga
      601 ccccggcgtt tgtgcgccgg gcgtgcgagg ccagcctcgc gcgtctggac acggaccaca
      661 ttgatctcta tcagttgcat aatccccgca tcgatacgct tcgcaaggat gacctgttcg
      721 ccgagctgga acggctcaag tccgagggca agatccggca ttacggggca accctgggac
      781 cagccatcga ccggcgccag atcgaagaag ggaagtgctg cgtggccgag cgacggatga
      841 tcacccagat catctacaac atgctggagc agcagatcgg ccggccgctc atcccggagg
      901 cgaagcagca tggggtgggc ctcatggtcc gggtgcccca ctcgtcgggc cttctcgagg
      961 gcaagtacac ccaggagacg acattcggaa cggatgacca ccggttcttc cgggtgaaca
     1021 cggacgaacg aaagaaggcc tggctggacg tgggcctgaa gaaggtgggg aagctcagct
     1081 tcctgacgga agggaaaggc cgaacgatcg cccaggccgc catccggtgg atcctggctg
     1141 agccctgctt cgcctgcgtc ctgcccaaca tctacaacga ggggcagctt cacgagttcg
     1201 ccggcgctgc ggatgttccg gacctcacgg cggaggaggt ccgtcaggtg aacgatctgt
     1261 acgagcgaaa cttcgacctg gagccggcgg cggtctaagc agagacgcag ggggggatcg
     1321 gagcgttggg gaaagggtga ctccagcctc gaacctcgaa cgttggacct tggacctcgg
     1381 acctcgaacg aagggacggc agatcatgtg cggcatcgtg gggtacatcg gggataagaa
     1441 ggttgttccc attctgctcg acggcctgaa gcgcctggag tatcgggggt acgactccgc
     1501 cgggctggcc gtcatcgagc agggccgaat ggtggtccac cgctccgtcg gaaggatcaa
     1561 ggagctggag aacgccgtct gggggagcaa cctgattggc accatcggtc tgggccacac
     1621 ccgctgggcc acccacgggc ggcccaccga ggaaaacgcc cacccccaca ccgactgcac
     1681 cggcgacgtg gtggtcgtcc acaacgggat catcgagacc tacctggccc tcaaggagaa
     1741 gcttctggcc gagggccatt tcttccgctc cgagaccgac accgaggtca tcgcccacct
     1801 catcgagaag tacctcaagg aggaccggga catcgaggtg gcaacgcggc gcgcgctgaa
     1861 cgatgtcacg ggcgcgtatg ccatcggcgt cctctggaag ggcgacccgg gccggatcgt
     1921 gggggccaag tgcggcagcc ctctggtggt cggattgggc gagggggatt tcttcttcgc
     1981 gtcggacatc ccggccatcc tgaatcatac gcggaacgtc ctcttcctcg acgatgaaga
     2041 gatcgtggtg ctgacaaagg acggcgtcca catcacgacg ctggcgggcg aggaggtgca
     2101 gcggtcggtg gagaaagtgc tctggagccc catcctggcg gagaagggcg gctacaagca
     2161 tttcatgctg aaggagatct acgagcagcc ccgcgccgtg cgggacacga tccgcggccg
     2221 gttctccgag gagagctccc agatcttctt cgaggggatg gaggcgctgg acgaggtcct
     2281 gccggagatc cagaaggtga ccctcatcgc ctgcggcacg gcctggcatg caggcttggt
     2341 tggcaagttc ctgatcgagg agctgtgccg cgtcccggtc gaggtggatt acagctccga
     2401 gttccgctat cgcgatccag tggtggaatc ccggacgctg gtcatggcgg tgagccagtc
     2461 cggggaaacg ctggacacgc tggccggcat caaggaagcg cggcagcgcg gctgccccac
     2521 cttcgccgtc tgcaacgtgg tgggaagctc catcacgcgg gaggtggacg gcgtcctcta
     2581 cacgcatgcc ggacccgaga tcggcgtggc cgccaccaag accttcaccg cgcagatcgc
     2641 ggccctctac ctcttcgccc tgtatttcgg gcggaagcga ggaatcgtgg acgcggcgcg
     2701 catgcgggaa ctcctggccg acctggttcg cctgccccag cagatcgagc aggtcc
//
LOCUS       DBNGZC010001005         5369 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_185100, whole genome shotgun sequence.
ACCESSION   DBNGZC010001005 DBNGZC010000000
VERSION     DBNGZC010001005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_185100"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="AC16G0_17320"
     CDS             <1..297
                     /locus_tag="AC16G0_17320"
                     /inference="COORDINATES: protein motif:HMM:NF014950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5101417.1"
                     /translation="DLPETIRSPKAKEGASLSMSLPDAGLSLEEVERELILRALQKHD
                     WNQSRAARYLGITRHTLLYRMEKHNIVRPGGRGGQAEDEAIERQGSGGAEGPRS"
     gene            323..1207
                     /locus_tag="AC16G0_17325"
     CDS             323..1207
                     /locus_tag="AC16G0_17325"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5101418.1"
                     /translation="MSEMTWGILGGLGCGLTWGMISLLVRSLSGAVTPVGITAVRSIL
                     GGGTLLSLALATGHGGEIAEMPLWVVLTLWGTIILSMGFGDTMFFASMDHLGVTRALT
                     LTMINPLLTTVVGVGLLGEPVTLPRGLGMLLVVGGLVLVIWGKGVGGGERHGSMQRGL
                     RLVCLAAGSWAISAVWMKPAFQAVSVIAAAAVRVPAAGLVLWLTPWTRGVFAAIALTT
                     RGERLLLGAICVLSAVASLFFTTGIKYGGVAVGNVLSATAPLFTLPFEVFVLGHHPSR
                     QTAVGAVVTIAGIGLMNL"
     gene            1314..2249
                     /locus_tag="AC16G0_17330"
     CDS             1314..2249
                     /locus_tag="AC16G0_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5101419.1"
                     /translation="MRVLVTGGAGFIGSHVADAYLQAGHTVGVVDDLSTGAKENLDPR
                     VRFWQADIRRAELWGILAEFRPEVVSHHAAQMSVSASVRDPRGDADINILGTLNLLEA
                     AVRHGVRRVVFASTGGAMYGEQPVLPTPETIFPEPVSPYGVAKLAVERYLHAFRVMHG
                     LPAVVLRYANVYWPRQNPHGEAGVVAIFCKAILEWRTLTINGDGDQTRDYIYVGDVVR
                     ANLLATQATVGPGVSSLNIGTGRETSVNELVRSLGDIAGGELPRRHAPPRPGEQQRSA
                     LDAALARRVLGWESTTNLGTGLAQTFRWFDGRGRR"
     gene            2676..3860
                     /locus_tag="AC16G0_17335"
     CDS             2676..3860
                     /locus_tag="AC16G0_17335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HIR5101420.1"
                     /translation="MNGSTSFDVLVIGAGPAGSTVAQQLAVGGFHVAVLEEHAVIGEP
                     VDCTGVIGTEAFADFDLPRGLVVGSIEAVTIHSPAAIPVTHRATAPLAYIVDRAELDR
                     TLATRAQTAGVTYHLSTQVVDVARTDRGVKVTCRRPDGEVCKLSGRVLVLAGGPRFAF
                     HERLGLKSCCLMWRSAHVELPGDGLPHAQVYLGRDVAPGAFAWAVPIKGSERQRVRLG
                     VNSHGDAPRYLRKLCEERFPHLIPRNEPFSYRTWVVPIHPLAETFGDRVLAVGDAAGQ
                     VKPTSGGGIYYGMLSARAAADTVSEAFTRGVLDREQLSTYEKRWRSRLSLDLKVGTLF
                     RRLFSRMTDRDVDDFFRALQADGLMARIAEKVSFDWHGALILFLLKHPSLARILLRRC
                     WD"
     gene            4169..5365
                     /locus_tag="AC16G0_17340"
     CDS             4169..5365
                     /locus_tag="AC16G0_17340"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5101421.1"
                     /translation="MRQQPRHPPIDVLVVGAGMHVCGRGTHTCGTVLPAIVQAHRDGL
                     VGRMLVAATSAKSIQAFDQKLAVLNQRLGTAAQFEGLPRAGRDPEVWRKALAECGERS
                     CVIIVVPDDLHGLIAGEAIRRRLPVLVEKPLTPTLAEAQALVNLARERGVYGAVDFHK
                     RWDLVNLKLKETMEDSRIGVPLYAVIEYSQRRVIPEEIFRSWVEQANPFQYLCVHFVD
                     VLHFALKAIPRRAMGVGQRCHLRRHSINAYDSVQACVEWVVPGSDVPFVSTFGANWID
                     PNTTSALSYQSLKVIGTLGRFESDQKDRGLRVITEADGIEDLNPYFTQSYPLADASRE
                     YRGYGIDSIRTFLEDVRRLHDGECAPSDLEPLRPTLRQALVSTAVVDAVNQSLCADGT
                     WVPIHV"
BASE COUNT          856 a         1712 c         1810 g          991 t
ORIGIN      
        1 gaccttcccg agaccatccg ctcgcccaaa gccaaggagg gcgcctccct gtccatgagc
       61 ctgcccgatg ccggcctgtc gctggaagag gtggagcgag agttgatcct gcgggcgctg
      121 cagaaacatg actggaatca gtctcgagcg gcgcgctacc tcgggatcac ccgccacacc
      181 ctgctgtatc ggatggagaa gcacaacatc gtccgacccg gtgggcgggg gggccaagcc
      241 gaggatgagg cgatagagag acaggggagc ggcggtgcag aggggccgcg gagctgagag
      301 acactgggat gcattttttt caatgagcga aatgacctgg ggcatcctgg gcggtctggg
      361 gtgcggcctg acatggggga tgatcagcct cctcgtccgc tccctgtccg gggcggtcac
      421 accggtcggg atcacggcgg tccgctccat tcttggtggc ggcaccctcc tgagcctggc
      481 tctggccacg gggcatgggg gcgagattgc ggagatgccg ctgtgggtcg tcctgaccct
      541 gtggggcacg atcatcctct ccatggggtt cggcgacacg atgttcttcg ccagcatgga
      601 ccacctgggc gtcacccgcg ccttgaccct gaccatgatc aatcccctcc tcaccaccgt
      661 ggtgggcgtc ggcctcctgg gggagcctgt gacgctcccg cggggactcg ggatgctcct
      721 ggtcgtcggc ggcctggtcc tcgtcatctg ggggaaaggg gtgggcgggg gcgaacggca
      781 cggctcgatg caacgtggtc tccgactcgt ctgcctggcc gcgggctcgt gggccatctc
      841 cgcggtctgg atgaagcccg ccttccaggc cgtgtcggtc atagctgccg cggcggtgcg
      901 ggtcccggcg gcgggtctgg ttctctggtt gacgccttgg acccgggggg tcttcgcggc
      961 catcgcactc acgacgcgag gggagcgcct gctcctcggc gccatctgcg tcctgagcgc
     1021 cgtcgcctca ctcttcttca ccacggggat caagtacggt ggtgttgcgg tgggaaacgt
     1081 cctttcggcc accgcgcccc tgttcaccct ccccttcgag gtgtttgtgc ttggacacca
     1141 cccctcccgg cagacggcag tcggcgccgt cgtgacgatc gccggcattg gcctcatgaa
     1201 tctgtgaaag cgtggtgatg tgggcggacg attccccgcc gttcatccca ctgacccact
     1261 ccgggggtgc acccgctcct ggcagcgggg gaagaaaatc agagaggagt tccatgcgag
     1321 tcttggtcac cgggggagca ggcttcatcg gctcgcacgt ggcggatgcg tatcttcagg
     1381 ccgggcatac cgtcggcgtg gtggatgacc tctccaccgg cgcaaaggag aacctggacc
     1441 cgcgggtgcg gttctggcag gcggacattc gccgcgccga gctgtgggga atcctggcgg
     1501 aatttcgacc cgaggtggtc agccatcacg ccgcccagat gagtgtctcg gcctccgtgc
     1561 gggacccccg aggcgatgcg gacatcaata tcctgggaac gctcaatttg ctggaggcag
     1621 ccgtccggca cggggtccgg cgggtggtct tcgcctcgac cgggggcgcc atgtacggag
     1681 aacagccggt cctcccgacg ccggagacga tcttccccga gccggtgagc ccgtacgggg
     1741 tggcgaagct ggcggtggag cggtatctcc acgccttccg ggtgatgcac gggctcccgg
     1801 cggtcgtcct ccggtacgcg aatgtctatt ggccccggca aaacccccac ggggaggcgg
     1861 gggtggtcgc catcttctgc aaggccattc tcgagtggcg gacgctcacg atcaacgggg
     1921 atggggacca gacccgcgac tacatctacg tcggggacgt cgtccgggcc aatctcctgg
     1981 ccacgcaggc gacggttggc ccgggagtct ccagcctcaa catcggcacg ggaagggaga
     2041 cgtccgtgaa cgagctcgtg cggtccctgg gcgacatcgc tggcggtgag ctgccgcgcc
     2101 gccacgcccc accccgtccc ggggagcagc agcggagcgc cctcgacgct gcccttgcca
     2161 ggcgggttct gggttgggag tcgacgacca acctcgggac gggtctggcc cagaccttcc
     2221 ggtggtttga cggcagggga cgccgttagc caagattact cacggaaaat tcgtgagtaa
     2281 tctggaacca atggccaatg acacaattcc aattaccaat cgccagaggg gggtcccacc
     2341 gctgtgtgca gggtcacgtg caccaagctc tccgctctcg atcattggcc attgggattt
     2401 ggtcactccc tcaggattcg ggagtcctgt tctcgaagat tgcggagcat aaggaaagcg
     2461 cagccggagg tcgcctggag acttcacatt cagttccctt cggctccgct gggtccacgg
     2521 agcatttgac atatccgggg catccgtgct aaaaggccgc cgttcccctt gtgcgtggac
     2581 gctgatcgtt cctcctattc tccatactcc ccctgtctag tcgcctcggc acctggggga
     2641 gcggatcgcg cagttctgac cgagggttcc cctgcatgaa tggatcgaca tcctttgatg
     2701 tcctcgtgat cggggcgggg ccggccggct ccaccgtggc acaacaactc gccgtcggcg
     2761 ggtttcacgt cgcggttctc gaagagcatg cggtcatcgg ggagccggtg gactgcaccg
     2821 gcgtgatcgg caccgaggcc ttcgcggact tcgatctgcc ccgaggtctc gtcgtcggct
     2881 ccatcgaggc ggtgacaatc cactcgcctg ccgcgattcc cgttacccac cgggccacgg
     2941 caccgctggc gtatattgta gaccgcgccg aattggaccg aaccttggcg acccgagcgc
     3001 aaacggccgg cgtcacgtat cacctgagca cccaagttgt ggatgtcgcc cggaccgacc
     3061 ggggtgtgaa ggtgacctgt cgccgtcctg atggcgaggt ctgcaagctc tccggaaggg
     3121 tgctggtcct ggcgggcggg cctcggttcg cgtttcacga gcgactgggc ctcaagtcat
     3181 gttgcctgat gtggcggagc gctcacgttg aactccccgg ggacgggctg ccccatgcgc
     3241 aggtgtatct gggccgggac gtggcccccg gtgcctttgc ctgggcggtc cccatcaagg
     3301 ggtcggaaag acaacgtgtc cgtctcgggg tgaacagcca cggagacgcc ccgcggtatc
     3361 tccggaagct gtgcgaggag aggttccccc atctcatccc caggaatgaa ccgttctcgt
     3421 accggacctg ggtcgtgccg atccaccccc tggccgagac cttcggggac cgggtcctgg
     3481 cggttgggga tgcggctggt caggtgaagc ccacctcggg cggcgggatc tactatggga
     3541 tgctcagcgc ccgggcagcc gccgacaccg tgtcggaggc attcacgcgt ggagtgctgg
     3601 accgcgagca gctctcgacc tacgagaagc gctggcggtc gcggctgagc ctcgacctca
     3661 aggtcgggac actcttccgg cgcctttttt cgcgaatgac cgaccgggac gtggacgatt
     3721 tcttccgggc gctgcaggcc gacggcctca tggcccggat agcggagaag gtgagcttcg
     3781 actggcacgg cgccctgatc ctgttcctgc tgaagcatcc ctcgcttgcg cgtatcctcc
     3841 tgcgccgctg ctgggactga cgggccgacc cccccctccg gcgcagagcc catggcccgt
     3901 tcagcctgcg gaattccact ggcatccgga ttcgcccgga cgtggtctct gttctccgtt
     3961 tcctcttcca gatgtcgcgc gtcgcatggt ttccattccc ccgtatccag tcggaaggac
     4021 gtccgcagag gaaagcgagc aggaacatgc ggggcgcggg atgattcacg tgtaagggtt
     4081 attgccgccc gcgccggttt tctgtagact cagcaagatt ccgtgcctca taggccgcgt
     4141 tcgaaatctt tcaataaggc ccttcgccat gcgtcagcag ccgcgccatc cacccatcga
     4201 tgtcctcgtg gtcggggccg gcatgcacgt gtgcgggcga gggacccaca cctgtgggac
     4261 ggttcttccg gcgatcgtgc aggcccatcg ggacggcctc gtcggacgca tgctggttgc
     4321 ggccaccagc gccaagtcca tccaagcatt tgaccagaag ctggcggtcc tgaaccagag
     4381 gcttggcacc gcagcccagt tcgagggcct cccccgagcg ggtcgcgatc ccgaggtctg
     4441 gcggaaggct ctggcggagt gtggggagcg atcctgcgtc atcatcgtgg tgcccgacga
     4501 cctgcacggg ctcatcgccg gcgaggcgat tcgccgccgg cttccggttc tggtggaaaa
     4561 gcccctcacg ccgaccctgg cggaggccca agccctggtg aatctggcac gggagcgtgg
     4621 ggtgtacggc gcggtggact tccacaagcg ctgggatctg gtgaatctga aactcaagga
     4681 gacgatggag gacagccgaa tcggcgtgcc tctgtatgcc gtcatcgaat acagccagcg
     4741 ccgggtgatc ccggaagaga tcttccggag ctgggtggag caggcgaatc cgttccagta
     4801 tctgtgtgtc catttcgtgg acgtcctgca tttcgccctg aaagccatcc cgcggcgcgc
     4861 catgggcgtg gggcagcgct gccatctccg acgacactcg atcaacgcct acgattcggt
     4921 gcaggcctgc gtcgagtggg tggtcccggg gtcggatgtg cccttcgtct ccacctttgg
     4981 ggcgaactgg atcgatccca acacgacctc ggccctgtcc taccagtcgc tcaaggtgat
     5041 cggaacgctg ggccgattcg agagcgatca gaaggaccgc gggctccggg tcatcaccga
     5101 ggcggacggc atcgaggatc tcaatccgta ctttacgcag agctatccct tggcggacgc
     5161 atccagggag taccgggggt atggcattga cagcatccga accttcctgg aagatgtccg
     5221 caggctccat gacggcgagt gcgcgccgag cgatctggaa ccgctgcggc cgaccctgcg
     5281 gcaggccctg gtgtccaccg ccgtggtgga cgcggtgaat cagagcctct gcgccgatgg
     5341 aacctgggtg ccgatccacg tgtaggcag
//
LOCUS       DBNGZC010001006         5124 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_770787, whole genome shotgun sequence.
ACCESSION   DBNGZC010001006 DBNGZC010000000
VERSION     DBNGZC010001006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_770787"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1397
                     /locus_tag="AC16G0_17345"
     CDS             147..1397
                     /locus_tag="AC16G0_17345"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR5101422.1"
                     /translation="MGKVRVLVCPKCQRQVAPAEKKFSCPECHVILDVQVDLDHLSAD
                     FQEKLLRRQDSTIWKWHEFMPVEDLSCVVSLGEGGTPLVHTEALAAATGLERLFIKND
                     TLLPTGSLKDRSNAVGISKAKEWGATTAAVVSTGNAAASVAAYAAVAGMQAVVIIPAS
                     TSPQKVAQAAIYGARIIPIEGSFEQAAGIYQAALEEFGWYNCLSSNPYRLEGKKSYAF
                     EVWEQLEGEAPDWMIHPTAGGAGVAAAYKGFRELKGLGWVEKLPRMVAAQADACAPVV
                     RAFEAGAEAITPVEAGETIAESIRVGKPSGMATRCLLEVRDSGGAAVGVTDDEIRSAQ
                     SLLARTAGVFGEPSGVASLAAALKLKAQGKIQAHELVVCTVTGHGLKQVGVLDPARLV
                     SRPIPATLTALRQRLREVANDDAA"
     gene            1387..2733
                     /locus_tag="AC16G0_17350"
     CDS             1387..2733
                     /locus_tag="AC16G0_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008454169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5101423.1"
                     /translation="MQRSTATPNIRVPPPGPNAQRWMAYHRAAAARATYVEGFVWDRA
                     APAIGPFCTDPDGNVFLDFASHVGSSPLGYNHPELLAVSDRLKSIDPDRYAGTDFVSA
                     YGKDPDRLDIPTPSHLHHKLLETTAPFDFGTAFFSNSGAEAVENAIKICYDARRNCGY
                     GFCFLGAFHGRTLGALSLNRSKRLHRSWYPQIPNIVHLPYCDCPDECHCGFWLGPPHS
                     MSQLAHFLDPAHGLIDPEEVAYIIIEPVQGEGGYNVPNPGFMREIGRIASQYHIPLIA
                     DEIQSGMGRTGRWWASEHFDIHPDIIVAGKALRVGATIGRTELFPKEEMRLGSTWGEG
                     NALSSAIGFTTIQVIQRDGLLAHATRMGERLRHELRSMQRRFDFIVGVRGLGLMDAVE
                     LDSVERRSRIVHACLERGFLLLGCGHTAIRFLPPLDLTERELGLALEILSEVLALV"
     gene            2903..3160
                     /locus_tag="AC16G0_17355"
     CDS             2903..3160
                     /locus_tag="AC16G0_17355"
                     /inference="COORDINATES: protein motif:HMM:NF046758.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FitA-like ribbon-helix-helix domain-containing
                     protein"
                     /protein_id="HIR5101424.1"
                     /translation="MATLYVRDMPDELYKHLKKRATEEGRSISQETVQLLRLALLTNR
                     PKPDLEFKAWIDRVAEQRERWVKEGRKFPNSTTLIREDRDR"
     gene            3157..3624
                     /locus_tag="AC16G0_17360"
     CDS             3157..3624
                     /locus_tag="AC16G0_17360"
                     /inference="COORDINATES: protein motif:HMM:NF013970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HIR5101425.1"
                     /translation="MIPVVLDTGVIVKIFVEDQDSSRATALLRAAAEGNFTLAAPDFM
                     AIEFGNVLWKYVRRSVLREEEAWQALGEFPYDRIEWLAARPLLKDAFKFAREYDVAVY
                     DGAFLAGAESLAVDFVTSDEALHRKVSHRLPWVKLLRDFDAGSAAGSTGHRTP"
     gene            3621..4856
                     /locus_tag="AC16G0_17365"
     CDS             3621..4856
                     /locus_tag="AC16G0_17365"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIR5101426.1"
                     /translation="MTPVRTIIHLDMDAFYASVEQLDHPAYRGKPVVVGADPHGGKGR
                     GVVAACSYEARPFGVRSALPISQAYRLCPQAVFVRPRMKRYAEMSERMFAILREFTDL
                     VEPLSIDEAFLDVTASQRLFGSAETIGRALKARIRTELGLVASIGLAPNKFLAKVGSD
                     LGKPDGFVVVPPGSEREFLAPLPISRLWGVGPKTEARLKRLGLQTIGQIARLGVEGLE
                     TALGNAGRDLWELANGMDDRPVVPEQEAKSIGAETTFEQDTDDREEVRKTILELSDRV
                     GRRLRREGYVAHGVTLKFRDHNFQTLTRASMLPHPTDLGEELFRAAATLLQGVKWAGK
                     RVRLLGVTATRLLPATGAPGDQLHLFQKAAEPKERLARTVDAIRERYGSEAITRASLV
                     SSTPTRKASKSARKDSLPR"
     gene            4919..>5124
                     /locus_tag="AC16G0_17370"
     CDS             4919..>5124
                     /locus_tag="AC16G0_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HIR5101427.1"
                     /translation="MKPQITQIAQISGSEMERDPRTFALIGAAMEVHRVLGPGFLEAV
                     YQEAFARELSQKDIPFRIEVELPV"
BASE COUNT          948 a         1557 c         1686 g          933 t
ORIGIN      
        1 ggggcttctc caattcaaac cgatgacatt gatgacagtt ggcgagtctt tgcgggcttg
       61 gcgatcttgg cgaggaaatt ttcggattga aaagagagaa tagaaaacag gcggagttcc
      121 tttcctagac aaaagccgag gaggccatgg gaaaagttcg agtgctcgta tgtccgaaat
      181 gtcagcggca ggttgccccc gcggagaaga agttctcttg tccggagtgc cacgtaatcc
      241 tggatgtgca ggtggaccta gatcacctat ccgccgactt ccaggagaag ctgttgcgcc
      301 ggcaggattc gaccatctgg aagtggcacg agttcatgcc ggtggaggat ctctcctgcg
      361 tggtgagcct gggtgaaggt gggacgcccc tggtgcatac cgaggccctg gcggccgcca
      421 cgggcctgga gcggctgttc atcaagaacg acacactcct gccgaccggc tcgctgaagg
      481 atcgcagcaa cgccgtgggc atctccaagg cgaaggagtg gggagccacg acggcagccg
      541 tggtgtccac gggaaacgcc gccgcctcgg tggcggccta cgcggcggtg gcagggatgc
      601 aagccgtggt aatcattcct gcatccacct cgccccagaa ggtggcccag gccgccatct
      661 atggggcccg tattattcca atagaaggaa gcttcgagca ggcggccggc atctatcagg
      721 cggctctgga ggaattcggc tggtacaatt gtctctccag caatccgtat cgcctggaag
      781 ggaaaaaatc ctacgccttc gaggtctggg agcaactgga gggggaggcg ccggactgga
      841 tgatccaccc caccgcggga ggcgccggtg tggcggctgc ctacaaaggc tttcgcgagc
      901 tgaaggggct aggttgggtg gagaagctgc cccgcatggt ggccgcgcag gcagatgcct
      961 gcgcccccgt ggtccgggcc ttcgaggccg gtgcggaagc gatcaccccg gtcgaggcgg
     1021 gcgagaccat cgccgagagt atccgcgtcg ggaagccctc cggcatggcc actcgatgcc
     1081 tcctggaggt ccgggattca ggcggggcag ccgtgggagt gaccgacgac gagatccggt
     1141 ctgcgcaatc gctcctggcc agaaccgccg gggtcttcgg cgaacccagc ggggtagcgt
     1201 cgctggccgc ggcgctcaag ctcaaggcgc aggggaagat ccaggctcac gaactggtcg
     1261 tctgcaccgt gaccggtcac ggtctgaagc aggtgggggt gctggacccg gcccgcctcg
     1321 tcagccggcc tatcccggcc acactcacgg ccctgcgcca gcgcctgagg gaggtggcga
     1381 atgacgatgc agcgtagcac cgccactccc aacatccggg ttccgccgcc tggtccgaat
     1441 gcccagcggt ggatggccta tcatcgggct gcagcggcgc gagcgaccta cgtcgaggga
     1501 ttcgtctggg accgggcggc gcctgccatc gggccgtttt gcaccgaccc tgacgggaac
     1561 gtcttcctcg actttgcctc ccatgtcggc agcagtcccc tgggctacaa ccatcccgag
     1621 ctcctggcgg tgagcgaccg gctgaaatcc atcgacccgg accggtacgc cgggacggac
     1681 ttcgtctcgg cgtacggcaa ggacccggac cggctggaca tcccgacccc gtcccacctc
     1741 catcacaagc tcctggagac cacggcgccc ttcgacttcg ggacagcgtt cttctccaat
     1801 tctggcgccg aggcggtgga gaacgccatc aagatctgct acgacgcccg gcggaattgc
     1861 gggtacgggt tctgcttcct gggggcgttt cacggccgga ccctgggcgc cctgtccctc
     1921 aaccgaagca agcggctgca ccggtcctgg tatccccaga ttccgaacat cgtccacctg
     1981 ccctattgcg attgccccga cgagtgtcac tgcgggttct ggctgggccc cccgcactcg
     2041 atgagccaac tggcgcattt tctggatccg gcccacggcc tcatcgaccc ggaggaggtg
     2101 gcctacatca tcatcgagcc agttcagggc gagggcggct acaacgtccc gaacccggga
     2161 ttcatgcgcg agatcggccg gatcgccagt caatatcaca tccccctgat cgccgatgag
     2221 atccagtcgg ggatgggtcg gacaggccgg tggtgggctt ccgagcattt cgatatccat
     2281 ccggacatca tcgtggccgg aaaggccctc cgggtgggag ccacgatcgg ccggaccgag
     2341 ctgtttccca aggaggagat gcggctggga agcacctggg gcgaggggaa cgccctcagc
     2401 tcagccatcg ggttcaccac catccaggtg atccagcggg acggcctcct ggcgcacgcc
     2461 accaggatgg gggagcgtct gcggcatgag ctccggtcca tgcagcgtcg ctttgacttc
     2521 atcgtggggg tccggggtct gggcctgatg gatgccgtgg agctggactc ggtggaacgc
     2581 cggagccgga tcgtacacgc atgtctggag cgcggcttcc tcctgctcgg ctgcgggcat
     2641 acggcgatcc gcttcctgcc acccttggac ctgacggagc gagagctggg tctggccttg
     2701 gaaatcctgt ccgaggtgct ggccctagtg tagctctctg tggcttactc acgcccatca
     2761 acgcggccct ttctccttcc ctggatccca acctgaatcg cttcgctaag taggttcatg
     2821 ttgacaggaa gttttggcgc ttgcatactg ctagcagaat aattgcattc agaggattta
     2881 gactgtcttc agggaggaag tcatggcaac gctttatgtc cgtgacatgc cagatgagct
     2941 ctacaagcac ctgaaaaaac gggcgaccga ggagggccgg agtatcagcc aagagacggt
     3001 tcaattgctc cggctcgctc tcctcactaa tcgaccgaaa cccgatttgg agttcaaggc
     3061 ttggatcgat cgggttgccg agcagcgaga acgatgggtg aaagaagggc ggaaattccc
     3121 gaatagtacg accctcattc gtgaggaccg cgaccggtga tacccgttgt cttggatacc
     3181 ggcgtaattg tgaagatttt tgtggaggac caggactctt caagagcaac agccctgctc
     3241 cgggcggcag ctgagggaaa tttcaccctt gcagcgccgg acttcatggc tatcgagttt
     3301 ggcaacgtct tgtggaagta cgttcgtcgt agcgtcctca gggaagagga ggcgtggcaa
     3361 gcactgggcg aatttcccta cgatcgcatc gagtggctgg ctgcccgtcc attgctcaag
     3421 gacgccttca agttcgcaag ggagtatgat gtcgctgtct acgatggcgc tttcctggca
     3481 ggggccgaaa gtctggcggt ggactttgta acgtccgacg aggccttgca tcggaaggtt
     3541 agccatcgcc tcccctgggt gaaactactc cgagatttcg atgcggggtc ggcagcggga
     3601 agtaccggcc acaggacccc atgactccgg tcaggaccat catccacctc gacatggatg
     3661 ccttctatgc gtcggtggag cagctcgacc acccggcgta tcggggaaag ccggtcgtgg
     3721 tgggcgctga tccacacggc gggaaagggc gcggcgtggt cgccgcctgc tcttatgagg
     3781 cgcgaccgtt cggggtccgc tccgccctgc cgatttccca ggcctaccgg ctgtgccctc
     3841 aggccgtctt cgtccgccct cgcatgaagc gctatgcgga aatgtcggag cgcatgttcg
     3901 ccatcctgcg agagttcacc gatctggtgg agcccctctc catcgacgag gcgttcctgg
     3961 acgtcacggc cagccagcgc ctcttcggat cggcggagac gatcggccgg gccctgaagg
     4021 cccggatccg caccgagctg gggctggtgg cctccatcgg tctcgcgccc aacaagttcc
     4081 tggccaaggt cggctcggac ctgggaaagc cggacgggtt cgtggtggtc cctcctggtt
     4141 cggagcggga attcctggcc cctctgccga tctcccggct gtggggggtg gggccaaaga
     4201 cggaggcgcg cctgaagcgg ctgggtctcc agaccatcgg acagatcgcc cggctgggtg
     4261 tcgaagggct ggagacggcg ctgggcaatg cggggcgcga cctgtgggag ctggcgaacg
     4321 gcatggacga tcgcccggtc gtgccggagc aagaggccaa gtccatcggc gccgagacca
     4381 ccttcgagca ggacacggat gatcgcgagg aggtccggaa gaccatcctg gaactgtcgg
     4441 atcgggtggg ccgccgcctg cgtcgcgagg gatacgtggc tcatggcgtg acgctgaagt
     4501 tccgggatca caacttccag accctgaccc gagcgtccat gcttccccac ccgaccgatc
     4561 tgggcgaaga gctgttccgg gcggccgcga cgctcctcca gggtgtgaag tgggccggaa
     4621 agcgcgtccg cctcctcggt gtgacggcca cccggctgct cccggccaca ggggcgcctg
     4681 gcgaccagct ccatctattc cagaaagccg cggaacccaa ggagcggttg gctcgcacgg
     4741 tagatgctat ccgggagcga tacggatcgg aggccatcac ccgggcctcg ctcgtctcga
     4801 gcacccccac ccgcaaggcg tcgaagtcag ccaggaagga ctccctccct cgatgacacg
     4861 tgcgagtcgg gatgatagaa ttggtgccag gcgggggggc cgaaataaga agactttatt
     4921 gaaaccgcag attacgcaga ttgcgcagat ttccggatcg gaaatggaaa gagaccccag
     4981 aacttttgcc ctgatcggtg cggcgatgga agtgcatcga gtcctgggcc caggcttcct
     5041 tgaggccgtc taccaagaag cattcgcgag agaactctct cagaaggata tcccctttcg
     5101 cattgaagtc gagcttccag tctt
//
LOCUS       DBNGZC010001007         8036 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1061323, whole genome shotgun sequence.
ACCESSION   DBNGZC010001007 DBNGZC010000000
VERSION     DBNGZC010001007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8036)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8036
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1061323"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="AC16G0_17375"
     CDS             complement(<1..997)
                     /locus_tag="AC16G0_17375"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose dehydrogenase family protein"
                     /protein_id="HIR5101428.1"
                     /translation="MKVSVIGTGYVGLVTGACLAEIGHQVVCMDDDSAKVEVLRQGSL
                     PIYEPHLEEMVRRCRSAGRLVFTADVTEAVREAAVIFICVNTPPLPNGEADLCYVELA
                     TRRIAEHATQSTLITEKSTVPVQTSVWIEKTLAIYSQRPGIEFDVASNPEFTREGMAV
                     EDFLHPDRIVVGVSNPRAEQTLRRLYAPIVEGRFRCPIHTDCRQDRAVPFLVTNLASA
                     ELIKHASNSFLAVKITFANALADICELAGADILQVVKGVGLDRRIGRAFLNAGIGFGG
                     SCFPKDLKAFVKIAEKNGYDFALLKEVDRINQGRRELVLEKLRRALWILRGKRIGL"
     gene            complement(1034..2005)
                     /gene="gmd"
                     /locus_tag="AC16G0_17380"
     CDS             complement(1034..2005)
                     /gene="gmd"
                     /locus_tag="AC16G0_17380"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013377091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR5101429.1"
                     /translation="MSPHTAVITGITGQDGSYLADFLLTQGYRVVGMVRRSSTENFER
                     IEHLRGRVEILQADLLDQLSLINLLRAVRPQEVYNLASMSFVPTSWEQPVLTGEFTAL
                     GVTRILEAVRLVDREIRVYQASSSEMFGKVQAVPQTEATPFYPRSPYGVAKLYGHWIT
                     VNYRESYDLFACSGILFNHESPRRGKEFVTRKVTDAVARIHAGTATTLPLGNLQARRD
                     WGYAADYVEAMWRMLQQPTPDDYVIATGETHSVQELCEVAFARVGRRWQDHVIVDPAL
                     VRPAEVDLLQGDAGKAKRVLGWQPKVGFRALVELMVDADLARQAGRA"
     gene            complement(2002..3063)
                     /locus_tag="AC16G0_17385"
     CDS             complement(2002..3063)
                     /locus_tag="AC16G0_17385"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004475 - mannose-1-phosphate
                     guanylyltransferase (GTP) activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HIR5101430.1"
                     /translation="MQRLFAVVLAGGSGERFWPLSTPSHPKQFPPLLEGRSFFQHTVE
                     RAARLVGETKVLVAPRRDLLPIVQEQLPGFPPENCLLEPLGRDTAACIGLAALRIERR
                     QPGAYMLVAPSDHYIRPFHAFEETIRHGLALLERVDGLVTLGIPPTRPETGYGYILAG
                     ESVPEYAAGRRCLRFTEKPDRTTAERFLADGRYYWNSGIFLWGAGAILKAMAQHLPEH
                     WACLEEIRQALGTADEAGVVERVFPRMARVSVDVGVLERAENVCMVPATFAWDDVGAW
                     SALHRIFPLDECGNAVVGRHVARDSHGCVVYGTTRAIGTIGVRDLVVVETPEGVLVCP
                     KDRAQEVKDLVRLLREKSS"
     gene            complement(3205..3570)
                     /locus_tag="AC16G0_17390"
     CDS             complement(3205..3570)
                     /locus_tag="AC16G0_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015225451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HIR5101431.1"
                     /translation="MRSRPLEHMAKPWGCFCTLEEGPGYKIKRLVIEPGHRLSLQKHR
                     CRAEHWVVVSGTAWIVCGDRRLRLRERECAVIPRQAWHRIENRGRKTLVIIEVQHGAY
                     LGEDDILRRQDDYGRIAKQ"
     gene            complement(3609..5063)
                     /locus_tag="AC16G0_17395"
     CDS             complement(3609..5063)
                     /locus_tag="AC16G0_17395"
                     /inference="COORDINATES: protein motif:HMM:NF014877.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohexomutase domain-containing protein"
                     /protein_id="HIR5101432.1"
                     /translation="MTEIRFGTDGWRGVIADDVTFANVRLVARAIARRVRAEAGGQEP
                     AHVLVGHDTRFLSREFARAAAEAMAGEGVRVSLAEAFAPTPAFSYAVVRLHAAGAVVI
                     TASHNPARYTGVKFKSSYGGSAPIVFTRGVEEEIGRLRAASREPRAEGNLEIRNSKFE
                     IESFDARGPWLDRLEELVDVSRIGRSGIRVALDVMYGAGQGLLAERLRRAGCTVRELH
                     ADLNPAFGGLHPEPIPAHLGEFIDSLQAWEGPGRRVGFAFDGDADRVAPVDEAGNVVT
                     PHQTLALLVRHMVQQRKERGTVVKSVNIGHMVDAEARVLGLPLVVTPVGFKFLAEAMR
                     QHDALVGGEESGGFAVRGHIPERDAGLIALLLLECMAMTGKSLGTLVREMEAEHGPHR
                     YGRLDLTLASLAERDRVVAAISLSPPDRLLSQPVRHVETMDGVKLTRADESWIMVRAS
                     GTEPLLRIYAEAPTDAEVGALLDWGRGLALGKTQ"
     gene            complement(5185..6258)
                     /locus_tag="AC16G0_17400"
     CDS             complement(5185..6258)
                     /locus_tag="AC16G0_17400"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5101433.1"
                     /translation="MRLQADPQAARVTLEHVTKRFGQALAVDDVGLVIEPGSLVTLLG
                     PSGCGKTTTLRIVAGLETATSGRVFIGDVEVTHLPPNVRDVTMMFQSYALFPHLSVFE
                     NVAYGLRVLKRPEAEIRKAVEDVLALVGMGGMGARSPSALSGGQQQRVALARSLVMQP
                     KVLLFDEPLSNLDAKLRKRMREEIRLLQQRLGITAIYVTHDQAEALAISDQIVVMDKG
                     RISQIGSPDELYRRPGNRFVAEFIGEANFLPARLEAMEDGQAQVLLGPQRLQVRSPAL
                     QPGPVTVLARPEAIRIRTQGTGLPGTVQKVSYLGSSADYTVETELGAVLITDYEMQGG
                     VLSAGTAVRLEFLAHGVYPLPGV"
     gene            complement(6255..>8036)
                     /locus_tag="AC16G0_17405"
     CDS             complement(6255..>8036)
                     /locus_tag="AC16G0_17405"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5101434.1"
                     /translation="AGCGLVLWLTVAAAVRPYAFGLGGILALTICVLVLAFGLSQGGY
                     IRADTFVVTCILALAFFVLLFVLYPIYAVLQHSVMDARGIRLGLFLETLRGYKAFWRV
                     LRNSLGLAVFVGIVSTLIGLAFALAATRSRSRITVLLRSFSLLPMITPPFVVGLAIIL
                     LFGLQGFVTSTLLGLRTRGIFGFPGLALAQTLSFAPVAFLVIASVVESVNPALEEASL
                     TLRAGPWETFWKVTLPLLRPGLANAFLLTIIESLADFGNPIVLGGEFEVLSTEIYFAI
                     IGRFDQTLAASLGIVLLVFSLTTFLLQQVWLGERSYVTVTGKPQSGKPMALPRGLEIG
                     LLGFCAFWCLSTLALYLFVIYGGFVEIWGINNAFTLRHYHQLLRDGMDALYTTLILAG
                     VSAPLTAAVGILLAYLVVRHQFPGRGVLEFTSMLSFAVPGTVIGIGYIMAFNQSPFLL
                     TGTATILVISFIFRNMPVGIRSGIAALAQIDRALEEASVTLRASFATTLRRIVLPLIR
                     PAIISALVFSFVRSVTAISAVVFLVSAKWQLSTKVILDQAENGRYGLATAYSTVLIVI
                     MALAIVLMYLLVGKTATRGGVEGGGLA"
BASE COUNT         1409 a         2767 c         2644 g         1216 t
ORIGIN      
        1 ggagcccgat gcgcttgccg cgcaggatcc agagggcccg ccgcagcttc tccagcacca
       61 gctcgcggcg cccttggttg atccggtcca cttccttgag cagggcgaag tcgtagccgt
      121 tcttctccgc gatcttgacg aaggccttca ggtccttggg gaagcaggag ccgccgaagc
      181 cgatcccggc gttgaggaac gcccggccga tgcgcctgtc cagccccacg cccttgacca
      241 cctggaggat atcggccccc gccagctcgc agatatccgc cagggcgttg gcaaaggtga
      301 tcttcacggc caggaaggag ttcgacgcgt gcttgatcag ttcggcgctg gccaggttcg
      361 tcacgaggaa gggcaccgcc cgatcctgcc ggcagtcggt gtggatcgga cagcggaacc
      421 gcccctccac gatgggcgcg tacagcctgc gcagggtctg ctcggcccgg gggttggaaa
      481 cgcccacgac gatccggtcc ggatggagga agtcctccac cgccatcccc tcccgggtga
      541 actcggggtt actggcgacg tcgaattcta tgccgggccg ctggctgtag atggccaggg
      601 tcttttcgat ccagacgctg gtctggacgg ggaccgtgga cttctccgtg atgagcgtgc
      661 tctgggtcgc atgctccgcg atccgacggg tcgccagctc gacgtagcag agatccgcct
      721 cgccattggg aagcggcggc gtgttcacgc agatgaagat cacggccgcc tcccggaccg
      781 cctccgtcac gtccgcggtg aacaccaggc gtccggcgga acggcaccgc cgcaccattt
      841 cctccaggtg aggctcgtaa atcggcaggc tgccctgtcg cagcacctcg accttggccg
      901 agtcatcgtc catgcacacc acctgatggc cgatctccgc caggcacgcc ccggtcacga
      961 gtcccacgta gcctgtgccg atcacgctca ccttcatggg taccgctccc tccttctccc
     1021 cgacgcccgg gggctaggcg cggccggcct gccgcgccag gtcggcgtcc accatcagct
     1081 cgacgagcgc gcggaagccc accttgggtt gccagcccag gacccgcttg gccttgccgg
     1141 catccccctg gaggagatcg acctcggcgg ggcggaccag ggcgggatcc acgatcacgt
     1201 ggtcctgcca gcggcggccc acccgggcga aggcgacctc gcagagctcc tggacggaat
     1261 gggtctcccc ggtggcgatg acgtagtcgt cgggggtcgg ctgttgcagc atgcgccaca
     1321 tggcctccac gtagtcggcc gcgtagcccc agtcccggcg ggcctggagg ttgcccaggg
     1381 ggagcgtggt ggccgtgccg gcgtggatgc gggcgacggc gtcggtgacc ttccgcgtga
     1441 cgaactcctt gccgcggcgg ggggactcgt ggttgaagag gatgcccgag caggcgaaga
     1501 ggtcgtagct ctcccggtag ttcacggtga tccagtggcc gtagagcttg gccacgccgt
     1561 aggggctccg ggggtagaag ggggtggcct cggtctgggg gaccgcctgg accttgccga
     1621 acatctcgct gctgctggcc tggtagaccc ggatctcgcg atccaccagc cggaccgcct
     1681 ccaagatccg ggtcaccccc agggcggtga actccccggt gaggaccggc tgctcccagg
     1741 aggtggggac gaaggacatg gaggccaggt tgtagacctc ctggggccgg acggcccgca
     1801 ggaggttgat gagggagagc tggtccagga gatcggcctg caggatctcc acccgccccc
     1861 gcaggtgctc gatgcgctcg aagttctcgg tgctggagcg gcggaccatg cccaccaccc
     1921 ggtagccctg ggtgaggagg aagtcggcca ggtaggagcc atcctggccg gtgatgccgg
     1981 tgatgacggc ggtgtgaggg ctcatgagga tttctcccgc aacaggcgaa ccaggtcttt
     2041 gacctcttgc gcgcggtcct tggggcagac cagcacaccc tcgggtgtct ccaccacgac
     2101 caggtcgcgc acgccgatcg tcccgatggc ccgggtcgtc ccgtacacga cacacccgtg
     2161 gctgtctcgg gccacatggc gcccgaccac cgcgttgccg cattcgtcca ggggaaaaat
     2221 ccgatggagc gcgctccagg cccccacatc gtcccaggcg aacgtcgcgg ggaccatgca
     2281 cacgttctcg gcccgctcca ggacgcccac atcgaccgac acgcgcgcca tccgggggaa
     2341 gacccgctcc accaccccgg cctcgtcggc cgtccccagg gcctgccgga tttcctcgag
     2401 gcaggcccaa tgctcgggga gatgctgggc catggccttg aggatcgccc ccgcccccca
     2461 caggaagata ccgctgttcc agtaatagcg gccgtccgcc aggaagcgct ccgccgtcgt
     2521 ccgatccggc ttctccgtga agcgcaggca gcgcctgccc gcggcgtact ccggcacgga
     2581 ctcgccggcc aggatgtagc cgtagcccgt ctctggccga gttggcggga tccccagggt
     2641 caccaagccg tccacccgct ccaggagcgc caggccgtgc cggatggtct cctcgaaggc
     2701 atggaacggg cggatgtagt gatccgaggg ggccaccagc atgtaggccc cgggttgccg
     2761 gcgctcgatg cggagcgccg ccaggccgat gcaggcggcc gtgtcacggc ccaggggttc
     2821 gaggagacag ttctctggcg gaaagccggg caactgctcc tggacgatgg gtagcagatc
     2881 ccgccgcggg gccaccagga ccttggtctc gccgaccagt ctggcggccc gctccaccgt
     2941 gtgctggaag aagctccgcc cctccaggag cggcgggaat tgcttcgggt gagagggtgt
     3001 cgacagcggc cagaaccgct ccccgctgcc gcctgccagg accaccgcaa acaatcgttg
     3061 catgctgtca ccttcgccca tcaagggaag tgtcgcagcc caactctggt atgcgaccct
     3121 ccgacgcgaa tgaccaatga caaatgacta agtcccaatg aaacccgcgt accctgccat
     3181 gggtcacttg gaatgggtca ttggtcattg cttagcgatg cggccgtaat catcctgccg
     3241 ccggaggatg tcgtcctcgc cgaggtaggc gccgtgctgc acctcgatga tgacgagcgt
     3301 cttgcggccg cgattctcga tccggtgcca ggcttggcgc gggatgaccg cgcactcacg
     3361 ctcccgcaac ctgagacgcc ggtcgccgca gacaatccag gccgtccccg atacgaccac
     3421 ccagtgctcg gcccggcaac ggtgtttctg gaggctcagc cggtggcccg gctcgatcac
     3481 cagccgcttg atcttgtagc ctgggccttc ctccaaggtg cagaaacagc cccacggctt
     3541 cgccatgtgc tccagcggcc gactcctcac ctgcgcagga tcaggcgctg cgggttgtgt
     3601 cttccggctc actgcgtctt ccccaaggcc aagccccggc cccagtcgag caacgcgccg
     3661 acctcggcgt ccgtcggggc ctcggcgtag atccgaagca ggggctccgt gcccgaggcc
     3721 cggaccatga tccagctctc gtccgctcgg gtcagcttga ccccgtccat ggtctctacg
     3781 tgtcggaccg gctgggacag caggcggtca ggtggcgaga ggctgatggc agccaccacc
     3841 cgatcccgct cggccagaga cgccagggtg aggtcgagac gcccgtaacg gtgggggccg
     3901 tgctccgcct ccatctcgcg gaccaaggtc cccagggact tgcctgtcat cgccatgcat
     3961 tccaggagca gcagcgcgat cagccccgcg tcgcgctcgg ggatgtggcc ccgcacggcg
     4021 aagcctccgc tctcctcccc acccaccagg gcgtcatgct gccgcatagc ctcggccagg
     4081 aatttgaacc cgacgggggt caccaccagg ggaagtccca ggacccgggc ctcggcatcc
     4141 accatgtggc cgatgttcac ggacttcacc acggtccccc gctccttgcg ctgctgcacc
     4201 atgtggcgca ccaggagcgc caaggtctgg tgcggtgtca cgacgttgcc ggcctcatcc
     4261 accggggcca cgcggtcggc gtccccgtcg aatgcgaagc cgacccggcg gcccgggccc
     4321 tcccaggcct gcagagagtc gatgaactcc ccgagatggg cagggatcgg ttccgggtgc
     4381 aagccgccaa aggcaggatt caggtctgca tggagttccc ggacggtgca tccggcgcgg
     4441 cgcagccgct ccgccaggag tccctgcccg gccccgtaca tcacgtccaa ggccacccgg
     4501 atgccgctcc tgccgatccg cgagacatcc acgagttcct cgagtcgatc cagccacggc
     4561 ccacgggcat caaaggactc aatttcgaat ttcgagtttc gaatttcgag attgccttcg
     4621 gctcgcggct cgcggctcgc ggctcgaagc cgtccgatct cctcttccac gccccgggta
     4681 aagacgatgg gggcggagcc gccgtagctc gacttgaatt tgaccccggt gtatctggcc
     4741 gggttgtggc tggccgtgat gaccacggcc cccgccgcgt gcaaccggac gaccgcatag
     4801 gagaaggctg gcgtcggggc gaaggcctcg gccagactca cccggacgcc ctctcccgcc
     4861 atggcctcgg cggcggcccg ggcgaattca cgagacagga atcgagtatc atggccgacc
     4921 agaacgtgcg ccggctcctg tccgccggcc tcggcgcgca cccgtcgggc gatggcccgg
     4981 gccaccaggc gaacattggc gaaggtcacg tcatcggcaa tgaccccacg ccacccatcc
     5041 gtcccgaacc gaatctcagt catgcgacat acccagaggc gttcgacacg cggcgtattc
     5101 tgcgcgagcc gcctcggccc gtcaagaaaa accagggcca gacgacgggg cattcagctt
     5161 tgggagtcga tgtctggcag aaggctacac acccggcaag gggtagaccc cgtgcgctag
     5221 gaactccagg cggacggccg tcccggccga caagaccccg ccttgcatct cgtagtcggt
     5281 gatcaggacg gctcccagct ccgtctcgac cgtgtagtcg gcggaggagc cgaggtagga
     5341 gaccttctgg acggtgccgg ggagaccggt cccctgagtt cggatccgga tggcctccgg
     5401 ccgggccagc actgtcaccg gcccgggctg gagcgcgggg ctcctcacct ggagccgctg
     5461 cggcccgagg agaacctggg cctgaccgtc ctccatcgcc tccagtcggg ccggcaggaa
     5521 gttggcctct ccgatgaact cggccacgaa ccggttgccc ggccgccgat acaactcgtc
     5581 cggcgagccg atctgcgaga tccgcccctt gtccatcacc acgatctggt ccgagatggc
     5641 cagggcctcg gcctggtcgt gggtcacgta gattgcggtg atccccagcc gctgctggag
     5701 caatcggatc tcctcgcgca tccgcttcct cagcttggca tccaagttgg aaagcggctc
     5761 gtcgaagagc aggaccttgg gctgcatgac caacgaccgg gccagcgcca cccgctgctg
     5821 ctggccgcct gaaagggcgc tgggactgcg cgcgcccatc ccgcccatgc ccaccagggc
     5881 caggacatcc tccacggcct tccggatctc cgcctcgggc cgcttgagca cccgcagccc
     5941 gtaggccaca ttctcgaaca cactgagatg tgggaacagg gcgtaggatt ggaacatcat
     6001 ggtcacatcg cgaacgttgg gtggcaggtg ggtgacctcg acgtccccga tgaacacgcg
     6061 accgctcgtg gcggtctcca gcccggccac gatgcgtagg gtcgtcgtct tcccacagcc
     6121 ggatggtccc aggagggtca ccagggatcc aggctcgatc accaagccca catcgtctac
     6181 cgccagcgcc tggccgaagc gcttggtcac gtgctccagg gtgacccgcg ccgcctgagg
     6241 atccgcctgc agtctcatgc cagacctccg ccctcaacac cgcctcgcgt tgccgtcttg
     6301 cctaccaaaa ggtacatgag cacgatcgcc agcgccatga tcacgatgag gaccgtgctg
     6361 tacgccgtgg ccaggccgta ccgcccgttc tccgcctggt ccaggatgac cttggtggag
     6421 agctgccact tggcgctcac caggaagacc acggcgctga tggcggtcac cgatcgcacg
     6481 aagctgaaga ccaatgcgga gatgatggcc ggccggatca agggcaggac gatccgcctg
     6541 agtgtcgtgg cgaaggaggc ccgcaaggtc accgacgcct cttccagcgc ccggtcgatc
     6601 tgcgccaggg ccgcgatacc gctccggatg cccacgggca tgttccggaa gatgaaggag
     6661 atcaccagga tggtggccgt cccggtcagg aggaagggcg actgattgaa ggccatgatg
     6721 tagccgatcc caatcaccgt gcccggcacc gcgaaggaga gcatggaggt gaattccagg
     6781 acaccccgcc ccgggaactg atggcggacc accaggtacg ccagcaggat ccccaccgcc
     6841 gcggtcagcg gcgccgagac cccggccagg atgagcgtgg tgtacaaggc gtccatcccg
     6901 tcccgcagga gttgatggta gtgcctcagg gtgaacgcgt tgttgatccc ccagatctcc
     6961 acgaacccgc cgtagatgac aaacaggtag agcgccaggg tggaaaggca ccagaaggcg
     7021 cagaagccca gcaacccgat ctccaggccc cgtggcaagg ccatgggctt ccccgactgc
     7081 ggcttgcccg tgaccgtgac gtaggatcgc tcgcccagcc agacctgctg taacagaaag
     7141 gtcgtcaggc tgaagaccaa gaggacgatc cccagggatg cggccagcgt ctggtcgaac
     7201 cggccgatga tggcgaagta gatctccgtg gagaggacct cgaactcgcc ccccaagacg
     7261 atggggttgc cgaaatccgc cagggactcg atgatggtga ggaggaacgc gttggccagc
     7321 cccggccgga ggaggggcag cgtcaccttc cagaaggtct cccacgggcc cgcccgcaag
     7381 gtcaagctgg cctcctccag ggccgggttg accgactcca ccacgctggc gatcaccagg
     7441 aaggccacgg gcgcaaagga aagagtctgc gccaaggcca gcccggggaa tccgaagatc
     7501 ccccgggtcc ggagccccag gagcgtgctc gtcacgaatc cctgcaggcc gaagagcagg
     7561 atgatggcca ggccgaccac gaaggggggc gtgatcatgg gcagcaggga aaacgagcgc
     7621 agcaggaccg tgatccgcga ccgggagcgg gtggcggcca gggcgaaggc cagaccgatc
     7681 agcgtcgaca cgattcccac gaagacggcc agccccaggc tgttccggag gacgcgccag
     7741 aaggccttgt agcctcggag ggtctccagg aacagcccca ggcggatccc ccgggcatcc
     7801 atcacgctgt gctggaggac ggcatagatg gggtagagga caaacagcag gacgaagaag
     7861 gccagggcca ggatgcaggt gacgacgaaa gtgtccgctc gaatgtaccc gccctgagac
     7921 aggccgaagg ccagcaccag cacgcagatg gtcagggcca ggatgccccc cagcccaaag
     7981 gcgtagggcc tgaccgcggc tgccaccgtc aaccagagca ccagaccgca gccggc
//
LOCUS       DBNGZC010001008         3389 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2041213, whole genome shotgun sequence.
ACCESSION   DBNGZC010001008 DBNGZC010000000
VERSION     DBNGZC010001008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2041213"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="AC16G0_17410"
     CDS             complement(<1..182)
                     /locus_tag="AC16G0_17410"
                     /inference="COORDINATES: protein motif:HMM:NF019228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13-like element transposase-related
                     protein"
                     /protein_id="HIR5101435.1"
                     /translation="MGDHDFPSAASGVIIPHGLYDVKRNDGHVNLGTSHDTSEFACDS
                     IEWWWESRGRTLYPRAT"
     gene            530..2224
                     /locus_tag="AC16G0_17415"
     CDS             530..2224
                     /locus_tag="AC16G0_17415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="COG3415 family protein"
                     /protein_id="HIR5101436.1"
                     /translation="MQRRYEICRAYFHEGATADQLAERFQLHVGTVRAIVRDFARDPD
                     VNSFFAAAQTGRKTAPKRTAIHERACALRRQGATLADIHAALDQEGFEVSESYLFRVL
                     RRAGLTATRHHRATRQPGEYAKDGSVVPEVADVRALSLEDGRQFPTQVAGLFLFLPVL
                     LDLDLPQAVTQAGLPGSEPIPPLQALLALLVPKLLGKRRVSHISDLCEDQGAGLFAGL
                     NVLPKVTYATDYSYKTERTMTEKLVAAVIAKTPLGDPPLSFNLDFHAIPFRGAEPDLE
                     NHWVPTRNRAGPAVMAFVAQAAGRRVLCYATANILRAETDGLVPKFAEYWKEQTGQYP
                     ARLLFDSRATTYAGLSQLTQRQVGFITIRRRGSGMLARVGRLPGDRWQCCQISQAKGQ
                     RRQVAYVDELVRLDGYEGTVRQLVVTGLGHESPTFFLTNDLPQPQTARDVIQTYASRN
                     HVENHLGEQITFFHLDCLCSEVRLNVDFDLTLTVLADLLYRGLAERLKGFAQAGPSRL
                     FRKFVDTPGQIEITANGVIVRLNKRAHNPLLKEAGLTQPTRAVPWLGGRNVRLVCP"
     gene            2516..>3389
                     /locus_tag="AC16G0_17420"
     CDS             2516..>3389
                     /locus_tag="AC16G0_17420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR5101437.1"
                     /translation="MPLVIKAVETHGRQDAYRLIGLSRDLRQTEDTIAWVIDELNDEQ
                     TGQDENYAFNLSMVLVEADPTLLLPRESAVLEARHFLPDLRAPLTERLRMLSWDSPTC
                     WQRLEEFCEEGKDKRYTNEVNLGHANRIVEALARYGQECEEKVRSVLSQKVNDYSHHP
                     MKWLEPLVVRLAGQAHLESTIPLLIAKLQEDADLLNEECAEALTRIDTPAVLHAVAEA
                     FPRAEHHFRLYATGPLEHIHSALAVETCSHLLGQEKDEDIRRELAHALLGHFAQEGIE
                     AARRLLVGRELDFES"
BASE COUNT          612 a         1189 c          989 g          599 t
ORIGIN      
        1 gtcgctcggg ggtagagcgt tcggccccgg ctctcccacc accactcgat gctgtcgcag
       61 gcgaactcgc tggtgtcgtg gctggtgccc aggttcacgt gaccgtcgtt ccgcttcacg
      121 tcatacaacc cgtgcgggat gatcacgccc gaggcggcgc tggggaagtc atgatcgccg
      181 attttcgcgg aggcccaact tgtctacttc gcacccaatt gtcttcgggc gaccgtatgg
      241 tttttcctgg ccctgacggt tcctgaagtc cgcggcatga ggaccccata ggcaagttct
      301 ccgcgattcc cgccccctcc gcgccgcctt tccctcgcct tcattccccc ctcgttcctg
      361 tccaatcccc tcctttcttg cctttctcgc acgccgccct tctcctactc ccaggatcaa
      421 actcctccca ggacgtccct tgggggcgga caacttgccg attcgggact caaccgcgat
      481 ggctcgctcc caagaactcg ctcgcctctt ccaacaacct cattccccgg tgcagcgccg
      541 ctacgagatc tgccgtgctt acttccacga aggcgcaacc gccgatcaac tcgccgagcg
      601 gttccagctc cacgtcggca cggtccgagc gatcgtccgc gacttcgctc gcgaccccga
      661 cgtcaactcc ttcttcgcag ccgcccagac cgggcgcaag accgccccga aacgcacggc
      721 gatccacgaa cgcgcctgcg cactccgccg ccaaggggcc accctggccg acatccacgc
      781 cgcactcgac caggaggggt tcgaggtcag cgaatcgtat ctcttccgcg tcctgcgccg
      841 cgccggcttg actgcaaccc ggcaccatcg tgcgacgcgg caaccgggcg agtacgccaa
      901 ggatggctcc gtcgtgccgg aggtcgccga cgtccgcgcc ttgtccctcg aggacggacg
      961 gcaattcccc acccaggtcg ccggcctctt cctgttcctt cccgtgttgc tggacctcga
     1021 cctgccccag gcggtcaccc aggcgggtct gcccggctcc gagccgatcc cgccgctgca
     1081 ggcgctgctc gccttgctgg tccccaagct cctcggcaag cgacgtgtca gccacatcag
     1141 cgacctgtgt gaggaccagg gcgcggggct gttcgcgggg ctcaatgtgt tgcccaaggt
     1201 cacctacgcg accgactact cctacaagac cgaacggacc atgaccgaga agctggtcgc
     1261 cgccgtgatc gccaagaccc cgttgggaga tcctccgctg agcttcaatc tcgacttcca
     1321 cgccatcccc tttcgcggcg ccgagcccga tctggagaac cactgggtcc cgacgcggaa
     1381 ccgggccgga cccgccgtga tggcgttcgt ggcgcaagcg gccggccggc gggtgctctg
     1441 ctacgcgacg gccaacatcc tgcgtgctga gaccgacggc ttggttccca agttcgcgga
     1501 atactggaaa gagcagacgg ggcaataccc cgcccgcttg ctcttcgatt cgcgcgcgac
     1561 cacctatgcc gggttgagcc aactgactca acgccaggtc ggcttcatca cgattcgccg
     1621 ccgtggctcg ggcatgttgg cgcgcgtggg ccgtctcccc ggggaccgct ggcagtgttg
     1681 tcagatctct caggccaaag gccagcgccg ccaggtcgcg tacgtcgacg aactggtccg
     1741 gctcgacggc tatgaaggta ccgtgcggca actggtcgtg acggggctgg ggcacgaatc
     1801 ccccaccttc ttcttgacca acgaccttcc acaaccgcag acggcccgcg acgtcatcca
     1861 gacctacgcc agccggaatc acgtagagaa tcatcttggt gaacagatca cgttcttcca
     1921 tcttgattgc ttgtgtagcg aggtacgcct gaatgtggac ttcgacctga cgttgacagt
     1981 gctggcggat ctgctgtacc gcggcctcgc cgaacggctg aaaggcttcg cgcaggcagg
     2041 cccgtccagg ttgtttcgga agttcgtcga cacaccgggc cagatcgaga tcacggcgaa
     2101 cggggtcatc gtccgtctga acaaacgggc gcacaatccg ctgctgaagg aagccgggct
     2161 gacgcaaccg acacgggccg taccgtggct gggcggtcga aacgttcgcc tcgtgtgtcc
     2221 ctgacgaccc cagaccgaga ctcgccgtgg tcatgcacgg cgatgtgtcc acccgttcag
     2281 gagtgccggg acagttgagt gtcgatcagg catgttatgc ctccgcgaaa atcggcggag
     2341 aacagtccct caccaggaag aggacttctg accgaacacc gccagagtga aggagcaaca
     2401 gccgtgcgct tacccgaagc ccagatccag gaagccatcc tccaccccga cccagagatt
     2461 cgggacaggg cgaccagcta ctttgcccag tcgtactccc ccgacccttc cctacatgcc
     2521 actggttatc aaggcggttg agacgcacgg cagacaggat gcctaccggc tgatcgggct
     2581 gtcgagggat ttgcggcaga ccgaagacac catcgcctgg gtgattgacg aattgaacga
     2641 cgagcagacc ggccaggacg agaactacgc cttcaacctg tcgatggtcc tcgtggaggc
     2701 cgaccctacc ctgcttctac ccagggaatc cgccgtcttg gaagcccgcc acttcctccc
     2761 cgacctgcga gcgcccctca ccgaacgcct gcgtatgctt tcctgggatt cccccacctg
     2821 ctggcagagg ctggaggaat tctgcgagga gggcaaggac aagcggtaca ccaacgaggt
     2881 caacctgggc cacgccaacc gcatcgtgga ggccctggct cggtacggcc aggagtgcga
     2941 ggagaaggtc cgttcggtcc tgtcccagaa agtcaatgat tacagccacc accccatgaa
     3001 gtggctggaa ccgctggtgg tgcggttggc cggacaggcg cacctggaat ccaccattcc
     3061 cctgctcatt gccaagctcc aggaagacgc cgacctcctc aacgaggagt gtgccgaggc
     3121 cctgaccagg atcgacacgc ctgccgtgtt gcacgccgtc gccgaggcat tccccagagc
     3181 cgaacaccac ttccgcctct acgccaccgg cccgctggaa cacattcact ccgccttggc
     3241 cgtggagacg tgttcccacc tgctcggcca ggagaaggac gaggatattc gcagggaact
     3301 ggcgcacgcc ctcttgggcc atttcgccca ggaaggaatc gaggcggcac ggcgactgct
     3361 cgtgggccga gaactcgatt tcgagagca
//
LOCUS       DBNGZC010001009         1550 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2339575, whole genome shotgun sequence.
ACCESSION   DBNGZC010001009 DBNGZC010000000
VERSION     DBNGZC010001009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2339575"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..856
                     /locus_tag="AC16G0_17425"
     CDS             218..856
                     /locus_tag="AC16G0_17425"
                     /inference="COORDINATES: protein motif:HMM:NF019228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13-like element transposase-related
                     protein"
                     /protein_id="HIR5101438.1"
                     /translation="MEDSIERFDTLIRSGARRLTGFQRRLFLAEVATELCGGSARQAE
                     RRFGWGRDTVEKGLHESLKGVRCLENFAARGRQRSEQKDPQLAADIRAIVEPHTQTDP
                     ELKSARRYTNLSAAEVRQALINRGYGDEELPAERTLRDILNRMNYRLKRIQKGKPLKK
                     TDETDAIFANVKAVRAQVRGDPKTLEISVDTKAKVALGDYARGGKNPDRRPR"
     gene            858..1445
                     /locus_tag="AC16G0_17430"
     CDS             858..1445
                     /locus_tag="AC16G0_17430"
                     /inference="COORDINATES: protein motif:HMM:NF019228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13-like element transposase-related
                     protein"
                     /protein_id="HIR5101439.1"
                     /translation="MTNGWDHDPPAKERLVPFGILMLASGALMLLFGSRETSDAWVDA
                     LQCWWRQVRSGLGHIKRLVIYLDNGPKNSGRRTQFLKRVVQFADWSGLEIRLVYYPPY
                     HSKYNPIERCWSALEQKWNGVLLNCLKVVLQCALRMTWKGQHPTVKRLYGVYPDGVRV
                     AAQERKPYEAPRQRSATLPKYDILIKPRVAESQGK"
BASE COUNT          305 a          460 c          525 g          260 t
ORIGIN      
        1 agcgacatct ggagccccgg agggcagggt gggcaggtcg aggcctgccc tctcaggggc
       61 gattggcaag gcccctgaac catattatga ccgggagtag ttgtttaggc gaccggtggt
      121 tggaagctta cctggaactg agctttcccg ccttgccaca tgatgcgaga ttatcggtcg
      181 atcgatcact gtctcgccgg cgaggagggc agccgtcatg gaagactcca tcgagcgctt
      241 cgacaccttg attcgttcgg gggccagacg gctcaccgga tttcaacgcc gcttgttcct
      301 ggccgaggtg gctacggagc tgtgcggcgg cagcgcccgt caggccgaac ggcgcttcgg
      361 ctggggacgc gacacggtcg agaagggcct gcacgagtct ctcaaggggg tgcgctgcct
      421 ggagaacttc gccgcgcgcg gccggcagcg tagcgaacag aaggacccgc aattggcggc
      481 cgacatccgc gccatcgtcg aaccccacac acaaaccgat ccggaactca agtcggcgcg
      541 tcggtacacc aacctgtcgg cggccgaagt gcgtcaagcc ttgatcaacc ggggctatgg
      601 cgacgaggaa ttgcccgccg agcgcacgct gcgggacatc ctcaatcgga tgaactaccg
      661 cctcaagcgg atccagaagg gtaagccgct gaagaagacc gacgagaccg acgcgatctt
      721 cgccaatgtg aaggcggtgc gggcgcaggt ccggggcgat cccaagacgt tggagatctc
      781 cgtggacacg aaggccaagg tggccctggg cgactacgct cgcgggggga aaaacccgga
      841 ccgacgccca cggtgacgtg acgaacggtt gggatcatga cccgccggcc aaggagaggc
      901 tggtcccgtt cggcatcctg atgctggcga gtggggcgtt gatgctcctg ttcgggtcac
      961 gggagaccag cgatgcctgg gtcgatgcgc tgcagtgctg gtggcggcag gtccgctccg
     1021 gcctcgggca catcaaacgc ctggtgattt acctggacaa tggcccgaag aactcgggtc
     1081 ggcggacgca gttcctgaag cgggtggtcc agttcgcgga ctggtcgggg ttggagatcc
     1141 gcctggtgta ctatccaccg taccacagca agtacaatcc gatcgaacgg tgctggtcgg
     1201 ccctggagca gaagtggaac ggcgtgttgc tgaactgctt gaaggtggtc ctgcaatgcg
     1261 ccctgcggat gacctggaag ggccagcatc ccaccgtgaa acgcttgtac ggcgtgtatc
     1321 ccgacggcgt tcgcgttgcg gcccaggaga ggaagccgta cgaggcccca cggcaacgtt
     1381 cggcgaccct gccgaaatac gacattctca tcaagccaag ggtcgccgag tcgcagggaa
     1441 agtaattctt gccggtcgcc taaggacgac atcgaccggt tctttgatgg ctaccatccg
     1501 aagaccggcg aggcccttgt gtcagcgggc gctcaaaccc agccaccgat
//
LOCUS       DBNGZC010001010         2530 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2728681, whole genome shotgun sequence.
ACCESSION   DBNGZC010001010 DBNGZC010000000
VERSION     DBNGZC010001010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2728681"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="AC16G0_17435"
     CDS             <1..634
                     /locus_tag="AC16G0_17435"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR5101440.1"
                     /translation="EQRKRRILLASVREEKAGHLASIKELERSAKDLQALIARLQSEE
                     ERQRRASRAAPQRQTPGGKEREDFPDIHDDGRFAQLKGELPWPAAGPLVSTFGRQEHP
                     RFHTITFNRGIEIGAPAGKDIAAVADGTVIYADWFKGYGRLLILDHGGGYFTLYAHAA
                     EILAKAGDSVSRGQVIGRVGDTGSLEGPQLYFELRHKGKPQDPVVWLQPR"
     gene            789..2147
                     /locus_tag="AC16G0_17440"
     CDS             789..2147
                     /locus_tag="AC16G0_17440"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR5101441.1"
                     /translation="MRRARHTGMLKGTIIAGTLILLLFTGGVQRGTTAVEDTYEKLKI
                     FTEVLSLVQSNYVDDVNSKDLIYGAVKGMLETLDPHSSFMPPEAFKEMQVETQGSFGG
                     LGIEITVKDRMLTVVAPIDGTPAEKAGIHPGDRIVKIEGAPTKDMTLMDAVRKLRGPR
                     GSKVTISILREGASEPVDVTLVREVIEVKSIRSKDLGNGMYYVRITSFQERTSKDLED
                     ALQQAKKAGANSLVLDLRNDPGGLLNQAVAVSDMFLDKGQLIVYTQGRQKNQDLRFTA
                     DHSNGLPRLPMVVLVNGGSASASEIVAGALQDWKRAVILGTKTFGKGSVQTVIPLSDG
                     SGLRLTTAKYYTPKGRSIHGVGITPDILVEQPRPELAKAQKPAEEQKTGEQLLQQGQV
                     KPPREQKIGDQEDQGVEIGRRDVVDITKDVQLQRAMEILKATGILEKNGTTQKAETKT
                     AG"
     gene            2177..>2530
                     /locus_tag="AC16G0_17445"
     CDS             2177..>2530
                     /locus_tag="AC16G0_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR5101442.1"
                     /translation="MELILGIESSCDETAAAVLEDGGRLRSNVISSQVELHAPYGGIV
                     PELASRRHLEVILPILDRALADAGIGRADVTALAVTVGPGLVGSLLVGVSVAKALAYA
                     RHLPLAAVNHLEGHIH"
BASE COUNT          486 a          762 c          891 g          391 t
ORIGIN      
        1 ggagcagcgc aagcggcgca tcctcctggc cagcgtgcga gaagagaagg cgggccacct
       61 ggcctcgatc aaggaattgg agcggtcggc gaaggatctc caggcgctga tcgccaggct
      121 ccagagcgag gaggagcgtc agcggagggc cagtcgggcg gcgccgcagc gccagacacc
      181 cggcgggaag gagcgggagg acttcccgga tatccacgat gacggccggt tcgcgcagct
      241 caagggggag ttgccctggc cggctgccgg gcccctcgtc tcgacattcg ggcggcagga
      301 gcacccccgg ttccacacca tcaccttcaa ccgggggatt gagattggcg cccccgcagg
      361 gaaagacatc gcggcggtgg cggacgggac ggtgatctac gccgattggt tcaaggggta
      421 cggtcgactg ctcatcctcg accacggggg cggctacttc acgctttacg ctcacgccgc
      481 cgaaattctg gccaaggcgg gagacagcgt ctcgcggggg caggtgatcg gccgggtggg
      541 ggacaccggg tcgctggagg gcccgcaatt gtatttcgaa ttgcgtcaca aggggaagcc
      601 acaagacccg gtggtctggc tgcagccccg ataggaccag gacggctgcc cgcgaaatcg
      661 cacgcgggag atgcagccgg aggagctgat ccgcattcat ctgtgggcac ggggggatcc
      721 cggccggcag tctggcgtgt tgcccgtctg ccggacgccc atggcatcgc acgagggagg
      781 gccgtttcat gcgtcgcgcg cggcacactg gtatgttgaa gggtaccatc attgccggta
      841 ccttgatcct gcttctcttc acgggtggcg tccagcgagg caccacggca gtcgaggaca
      901 cctacgagaa gctcaagatt ttcaccgagg tcctctccct ggtccagtcc aactacgtgg
      961 acgatgtaaa ttccaaggac ctgatctacg gggcggtcaa ggggatgctg gagaccctgg
     1021 acccccacag ctccttcatg ccacccgagg ccttcaagga gatgcaggtc gagacccagg
     1081 ggagcttcgg cgggctgggc atcgagatca cggtgaagga ccggatgctc accgtggttg
     1141 cccctattga cgggacgccg gccgagaagg cggggatcca tccgggggac cggatcgtca
     1201 agatcgaggg ggcgccgacc aaggacatga ccctgatgga tgccgtgcgc aaactccgcg
     1261 ggcccagggg ttccaaggtg acgatcagca tcttgcggga gggcgcctcg gagcctgtgg
     1321 acgtcacctt ggtgcgcgag gtgatcgagg tcaagagcat ccgcagcaag gaccttggca
     1381 acgggatgta ctatgtccgc atcacctcct tccaggagcg gacctccaaa gacctggagg
     1441 acgcgctgca gcaggccaag aaggccggcg ccaactcgct ggttctggac ctgcgcaatg
     1501 acccgggtgg tctcctgaac caggcggtgg cggtgagcga catgttcctg gacaagggcc
     1561 agctcatcgt ctacacgcag ggtcgccaga agaaccaaga tctccgcttc accgcggacc
     1621 acagcaacgg gttgccccgg ctgcccatgg tggtgctggt gaacggcggc tcggcgagcg
     1681 cctccgagat cgtggcgggc gccttgcagg attggaagcg ggcagtgatc ctggggacca
     1741 agacctttgg caaggggtcg gtgcagaccg tgatcccgct gtccgatgga tcgggcctgc
     1801 gcctgaccac ggccaaatat tacaccccca aggggcggtc catccacggc gtcgggatca
     1861 cgccggacat cctggtggag caaccccggc cggagctcgc caaggcgcag aagccggcag
     1921 aggaacagaa gacgggagag caattgctcc agcaggggca ggtgaagccc ccgcgggagc
     1981 agaagatcgg cgaccaggaa gaccagggcg tggagatcgg gcgccgggac gtggtggaca
     2041 tcacgaagga tgttcagctc cagcgcgcca tggagattct gaaagccacg gggattctgg
     2101 agaagaatgg caccacgcag aaggccgaga ccaagacggc cggctgagct tattatatgg
     2161 atctagcgta tcacacatgg agttgatcct cgggattgag agttcctgcg acgagacggc
     2221 cgccgccgtc ctggaagacg gcggccgtct ccgcagtaac gtgatttctt cacaggtcga
     2281 gctgcacgcc ccgtacgggg ggatcgtccc cgaactcgcg tcccgccggc acctcgaggt
     2341 gatcctcccg atcctggatc gagccctggc agatgccggg atcggccggg cggacgtgac
     2401 ggccttggcc gtcacggtgg ggccgggact ggtgggatcg ctgctggtgg gtgtttcggt
     2461 ggcaaaggcg ctggcctacg cccggcacct tccgctggct gcggtcaacc acctcgaagg
     2521 gcacatccac
//
LOCUS       DBNGZC010001011         1667 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2142852, whole genome shotgun sequence.
ACCESSION   DBNGZC010001011 DBNGZC010000000
VERSION     DBNGZC010001011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2142852"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(271..717)
                     /locus_tag="AC16G0_17450"
     CDS             complement(271..717)
                     /locus_tag="AC16G0_17450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5101443.1"
                     /translation="MSIVHRRQESRVEGVIMLRRVLVIDDDPDVCPLVSAMLEPGNYE
                     VHGASGGESGIVLADAEVPDLILLDLQMPGMDGYQVCRLLREGERTNRIPILMLTASD
                     DPALNRTAYAAGAHACILKPFRREALVAVIEAALTRPPRAKAPPGQ"
     gene            complement(763..>1667)
                     /locus_tag="AC16G0_17455"
     CDS             complement(763..>1667)
                     /locus_tag="AC16G0_17455"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5101444.1"
                     /translation="RELVEAILSQDGFDVELAADGTEALARATANPPDLILLDVLMPG
                     MTGLEACRRLKQTPATQTVPIIIVTAVAQVATKEAALTSGADDFMTKPIRPDDLRARV
                     VAMLKVRRIHQELDRTLTYLHELEAARHAQRQAVLDQVVAEPSPVTPQPSQPISILLV
                     DDEVLTRDFYGDLLAEHGFRVFAASSGPEGLTLARQHAPEVVILDILMPDMSGLEVLE
                     RLREEHPSVPVIMLTGRPTSQNAIASLKLGAFDFIVKGLNHSLVVMAVHRAVRYRRET
                     LQQQAAVQRLQARIADLEKTAAQA"
BASE COUNT          292 a          548 c          532 g          295 t
ORIGIN      
        1 catggtcacc accctgcggc agatgcaccc ttcctggaag atctgaacgc cggctcaacc
       61 tgtggtatgc ctgccatctg cagccgtgac gcttcccgtc ttcccgcgca aaatttcccg
      121 ccccccgccg ctactcgatt catgatcgtc ccttcctcag tcaaccggcg atgctgccgc
      181 catacgcttt gcctggttgc cttggcgacc agggaaccca gcgcctgccc gaagtgagcg
      241 cagcagggaa acccgcctcg cggattgggg ctactggccc ggcggcgctt tcgctcgcgg
      301 cgggcgggtc agggcggcct cgattaccgc gacgagcgct tccctccgaa agggcttcag
      361 gatgcaggcg tgcgccccgg cggcgtaggc ggtgcgattc agggccggat catcgctcgc
      421 agtcagcatg aggataggga ttcgattcgt acgttcgccc tcacgcagga gccggcagac
      481 ctggtagccg tccatccccg gcatctgcaa atcgaggagg atgaggtccg gcacctcggc
      541 gtcggccaag acgatgccgc tttctccgcc gctggctcca tgcacctcgt agttcccagg
      601 ctccaacatg gcggagacaa gcgggcagac atcgggatca tcgtctatca ccagcacgcg
      661 ccgcagcatg atcactccct ccaccctgga ctcttgacgg cgatgcacga tgctcacttg
      721 cacgtctcgt ggagcaagca tagggcaacc ccaagtttca aatcaagcct gggctgcggt
      781 cttctccaaa tcggcgatgc gggcctggag ccgctgaacc gccgcctgct gctggagagt
      841 ctcccggcga tagcgcacgg cccggtggac cgccatgacc accaggctgt gattcagacc
      901 tttcacgatg aaatcgaagg cgccgagctt cagggaggca atggcattct gggaggtggg
      961 gcgcccggtg agcatgatga ccggcacgct gggatgctcc tcccggagtc gctccaggac
     1021 ctccagaccc gacatgtcgg gcatcagaat gtcaaggatc acgacttccg gtgcgtgctg
     1081 gcgggccagg gtgaggcctt cgggtccgga actggcggcg aagacgcgaa acccgtgctc
     1141 cgccaggagg tccccataga agtcccgggt gaggacctcg tcatccacca gcagaatgga
     1201 aatgggttgt gagggttgcg gggtgaccgg agaaggctcc gcgacgactt gatccaagac
     1261 cgcctggcgc tgagcgtgac gggcggcctc gagttcgtgg aggtacgtca acgtgcggtc
     1321 cagttcctgg tggatccggc ggaccttcag catcgctacg acccgggccc gcaggtcgtc
     1381 cggccggatc ggcttcgtca tgaagtcgtc ggccccgctt gtcagcgcgg cctccttggt
     1441 ggccacttga gcgaccgcgg tgacaatgat gatgggaacg gtttgtgtgg caggggtctg
     1501 cttcagccgc cggcacgcct cgagtcccgt catcccgggc atcaacacat ccagaaggat
     1561 cagatccggc gggttggcgg tcgcccgggc caaggcctca gtcccgtcgg cggccagctc
     1621 cacgtcgaaa ccatcctgcg acaggatcgc ctccacgagt tcccgat
//
LOCUS       DBNGZC010001012         2632 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2529515, whole genome shotgun sequence.
ACCESSION   DBNGZC010001012 DBNGZC010000000
VERSION     DBNGZC010001012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2529515"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="AC16G0_17460"
     CDS             complement(<1..603)
                     /locus_tag="AC16G0_17460"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014114.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HIR5101445.1"
                     /translation="MLAKPERLRRGDRVRLIAPASPFDPALLRAGRRVLEDLGLIAVV
                     SRDEFARQGFLAGSDHRRAENLKRALQEEETKAVWCIRGGYGTARVLSLLDLAHLRRH
                     PKLLVGFSDVTALLVQLARPGGFVTISGPVITQLPRLPATDLRWLKALLFRTRAPQRL
                     PLGRTRTLVPGKAEGTLVAGNLSILASLAGTPFAPDLHDAV"
     gene            complement(872..>2632)
                     /locus_tag="AC16G0_17465"
     CDS             complement(872..>2632)
                     /locus_tag="AC16G0_17465"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR5101446.1"
                     /translation="RLFANQAAIAIANARLFRAERDRRAQLEAVRGVSADIAQELELN
                     KVLHLVAQRACELTRATASDIALWDPERQVLLSEASFGHAVPHPASPRRLGEGAMGTV
                     AEKRHGMIINDYRSSPIAHPDTLAHTKITASLVEPLLYRDTLLGVIGVDHETPGEVFT
                     SGDQALLRLFADQAAIAIANARLFKDQQRAFAALERAQDALIRTEKLRGLGQMAAGIA
                     HDLNNMLATILGQVELLRLRPHSPEVADGLQVLQTATNDGALVVRRLQDFARQRGGAP
                     LGACDLTRLVPEALEITRPRWREEPSRKGIVIETVVDLAGLPLIQGNPAEIREALTNL
                     IFNAVDAMPRGGRLRFTGEEVDGSNAGRPGELPGDPPTRASEPSWVELSVSDTGIGMT
                     EDVRRNAFDPFFTTKGPHGTGLGLSVVYGIMERHRGQIEVTSAPGQGTTFRLRFRSAP
                     TDTTQSAQRPMQALVPGRRILVVDDDASVRRTLAALLQASGQEVIQADGGAAALDCLG
                     TTPVDLVLTDLGMPDISGWDVARAAKARNPDLPVVLLTGWGDQVGAEAPSATPVDRVL
                     SKPVPRSTVLAVIAELTMGR"
BASE COUNT          408 a          880 c          933 g          411 t
ORIGIN      
        1 cacggcgtcg tggagatccg gggcaaaggg cgtccccgcc aggctggcca gtatagagag
       61 attgccggcc accaacgtcc cctcggcctt ccctgggacc agggtgcgcg tccggccaag
      121 cggcagacgc tgtggcgctc gtgtcctgaa gagcaacgcc ttgagccagc gcaggtcggt
      181 tgccggcagc cggggcaact gggtgatgac cgggccggag atagtcacaa agccgccggg
      241 tcgcgccaac tgcaccagca gggcggtgac gtccgagaat ccgaccagca gcttcgggtg
      301 ccgtcgcagg tgtgccaggt cgagcaagga gagtactcga gccgtcccgt agccaccgcg
      361 gatgcaccac acggccttgg tctcctcctc ttgcagcgcc ctcttcaaat tttcggcgcg
      421 gcgatgatcg ctcccggcca ggaatccctg tcgcgcaaac tcgtcccggc tcaccaccgc
      481 gatgagaccg aggtcttcga gcacccgccg cccggccctg agcaatgcgg gatcgaaggg
      541 gctggcaggc gcgatcaggc ggacccggtc ccccctccgc aggcgctctg gtttggcaag
      601 catgggccga cccatgaatc atcctcgcgc tgacatccaa acacgcagcc gtgccgaccc
      661 ggatccggca cccccagcct ggatgcgttc cccgttccgg tttccggctg ctggagatgc
      721 tcggcgagac cgcgatgccc tggtcgacca aggccgagta acggcgtgaa ccgagaggat
      781 atcgttgtta ggtaccgcca gcctggcctg ggtgtcaagc ggcaatcgcg gatctgccga
      841 tgttagcaac tcgcggccat gggccgggtc tctatcgccc catcgtgagt tccgcgatca
      901 ccgccagcac cgtgctccgc ggcacgggct tgctcagcac gcgatccaca ggggtggccg
      961 agggcgcctc tgcgcccacc tggtcccccc agccggtgag gaggacaacg gggaggtccg
     1021 ggttgcgcgc cttggcggcc cgcgccacgt cccagccgga gatgtcgggc atgccgaggt
     1081 cggtgagcac gaggtccacg ggagtagtcc cgaggcagtc gagggcggcg gcgcctccat
     1141 ccgcctggat cacctcctgg ccgctcgcct ggagaagggc ggccagggtt cgccggacgg
     1201 aggcgtcatc atccacgacc aggatccggc ggccgggcac cagggcttgc atcgggcgct
     1261 gcgcggactg cgttgtgtcc gtgggggcag accggaagcg gaggcgaaag gtggtgccct
     1321 ggccaggggc cgaggtcacc tcgatctggc cgcggtgccg ctccatgatg ccgtacacca
     1381 cagagagtcc gagcccggtg ccgtgcgggc ccttggttgt aaagaaggga tcgaaggcgt
     1441 tccgtcgcac atcctcggtc atgccgatcc ccgtatccga caccgacagc tccacccacg
     1501 agggctcgga cgcccgggtg ggagggtcgc cgggcagctc cccgggacga ccggcgttgg
     1561 atccatccac ctcctcgccc gtgaagcgaa ggcgcccacc tcttggcatg gcatccacgg
     1621 cgttgaaaat gagattcgtg agggcctcgc ggatctccgc ggggttaccc tggatcagcg
     1681 ggagaccggc gaggtccacg actgtctcga tgacgattcc cttccggctc ggctcctccc
     1741 gccagcgggg ccgggtgatc tccagcgcct cgggcaccag acgcgtcagg tcgcaggcgc
     1801 ccaggggagc cccgccgcgc tgccgggcga agtcctgcag acgccgcacc accagggccc
     1861 cgtcgttggt cgccgtctgt agcacttgca acccatccgc cacctcgggg gagtgtgggc
     1921 gcagacgcag cagttcgact tggcccagga tggtcgccag catgttgttc aggtcgtgcg
     1981 cgattcctgc ggccatctgc ccgaggccgc ggagcttctc ggtgcggatc agtgcgtcct
     2041 gggcccgctc aagggcggcg aaggctcgct gctggtcctt gaagagccgg gcgttggcga
     2101 tggcgatggc cgcctgatcc gcgaagagcc ggagcaacgc ctggtccccg ctggtgaaga
     2161 cctcgccagg tgtttcgtgg tcgacgccga tcacgcccag gagcgtgtcg cgatacagga
     2221 gcggctccac cagggaggcg gtgatcttcg tgtgggcgag ggtatcgggg tgggcgatgg
     2281 gactcgagcg gtagtcgttg atgatcatcc cgtggcgctt ctcggccacg gtgcccatgg
     2341 cgccctcccc cagccggcgc gggctggcag gatgggggac ggcatggccg aaggaggctt
     2401 ctgagaggag gacctggcgc tctggatccc agagcgcgat gtcggaggcg gtcgcgcggg
     2461 tgagctcgca tgcgcgttgc gccacgaggt gcaggacctt gttcaactcg agttcctggg
     2521 cgatgtccgc gctgacgccg cgaaccgcct cgagctgcgc gcgccgatcg cgctccgccc
     2581 ggaagagccg ggcattggcg atggcgatgg ccgcctgatt ggcgaagagc cg
//
LOCUS       DBNGZC010001013         2555 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1158217, whole genome shotgun sequence.
ACCESSION   DBNGZC010001013 DBNGZC010000000
VERSION     DBNGZC010001013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2555)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2555)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2555
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1158217"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="AC16G0_17470"
     CDS             <1..644
                     /locus_tag="AC16G0_17470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HIR5101447.1"
                     /translation="AQVDRFLPKYNRGDKQLHPDNPITIAPQANEDWVMEIRKQNDEA
                     MRRAQGVIQEAYQDFHQIFGRQGGSPFVEEYMTEDADVILFGQGTLSLPVKVGVRKLR
                     EQGHKVGFVRLKWLRPFATQEVQGALQRAKAVGVVDRDYSFGSPNHGAVLYNEVRSAL
                     YDLPQRPHVQSFICGLGGREVTLDSVTEMSEILLKTAKTGRMDQPNYWIGVRG"
     gene            650..1702
                     /locus_tag="AC16G0_17475"
     CDS             650..1702
                     /locus_tag="AC16G0_17475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012021147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR5101448.1"
                     /translation="MPTELTVLPTQILQAFKGVKKVTPEEYFTSGHRTCQGCESALVM
                     KLMAKAAGPRTVILGSTGCMYVANTTYYSTSWVVPWMHTQLGASGSAAMGTAAGLKAL
                     MRKGRIKQEPINVIAFCGDGGGADMGLSSISAALTYPEYNFLILMYDNESYANTDIQL
                     SGMTPYGANTTFSPPGKARRLLHRRWKKNVAGMLAAGHPECRYVATVDASYALEMMNR
                     VRKALSIGGPTFIHSLDPCPKGWDYDPYISHELGELAVETGVWPLFEVEDGILKFYGK
                     SKGIAQGRKRKPVREYLLKQGRFAHFLDEDIEYFQKKIDQMWDRWLVPGVCAFDMAED
                     LKKALDKSEAELEAVE"
     gene            2025..>2555
                     /locus_tag="AC16G0_17480"
     CDS             2025..>2555
                     /locus_tag="AC16G0_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5101449.1"
                     /translation="MNNAAQNKAVLVNRWWQLAACIVAMMAIANLQYAWTLFTIPLTK
                     SLHVKLSAVQIAFTLFILTETWLVPFEGYLVDRLGARLVVTVGGLLVGISWVWSGLAQ
                     TLHSLYLAYALGGIGAGAVYGACVGTALKWFPDRRGLAAGATAGAYGIGTALTVIPIN
                     RMIAGSGYQHAFIVWGV"
BASE COUNT          472 a          827 c          808 g          448 t
ORIGIN      
        1 aggcgcaggt ggaccggttc ctccccaagt acaaccgggg ggacaagcag ctccaccccg
       61 acaaccccat caccatcgcc ccccaggcca acgaggattg ggtcatggaa atccggaagc
      121 agaacgacga ggccatgcgg cgagcccagg gagtgatcca ggaggcctat caggatttcc
      181 atcagatctt tggtcgccag ggtggtagcc cctttgtcga ggagtacatg accgaggatg
      241 ccgatgtgat cctcttcggc cagggcacgc tctccctgcc ggtcaaggtc ggagtgcgga
      301 agctccgcga gcagggacac aaggtggggt tcgtccgcct gaagtggctg cggcctttcg
      361 cgacgcagga agtccagggg gcgctccagc gggccaaggc ggtgggcgtc gtcgaccggg
      421 attattcctt cggctccccc aaccacggcg ccgtgctcta caacgaggtc cgctccgccc
      481 tgtacgatct cccgcagcgg ccgcacgtgc agagcttcat ctgcggcctc ggcggccgcg
      541 aggtcaccct ggacagcgtg accgagatga gtgagatcct gctcaagacc gccaagacgg
      601 gcagaatgga tcagcccaat tactggattg gagtgagggg gtaatcgcca tgccgacaga
      661 gcttaccgtg ctgccaaccc agatcctgca ggcattcaaa ggggtcaaga aagtcacccc
      721 ggaagagtac ttcacctccg ggcaccggac ctgtcagggc tgcgaatccg ccctggtcat
      781 gaagctcatg gccaaggccg ccggccctcg gaccgtgatc ctgggctcca ccgggtgcat
      841 gtacgtggcc aacaccacct actattcgac ctcctgggtg gtgccctgga tgcacaccca
      901 gctcggggcc tcagggtcag ccgccatggg aaccgccgct gggctcaagg cgctcatgcg
      961 taagggccgg atcaagcagg agcccatcaa cgtcatcgcc ttctgcggcg atgggggcgg
     1021 ggcggatatg ggcctctcct ccatctcggc ggccctgacc taccccgagt acaacttcct
     1081 gatcctgatg tacgacaacg agtcctatgc caacaccgac atccagcttt ccggcatgac
     1141 tccctacggg gccaacacca ccttctcacc gcccggcaag gcccgccgac tgctccaccg
     1201 gcgctggaag aagaacgtgg ccgggatgct ggctgctggc cacccagagt gccggtacgt
     1261 ggccacggtg gatgcctctt atgccctgga gatgatgaat cgcgtccgga aggcccttag
     1321 catcggcgga cccaccttca tccactcctt ggacccctgc cccaagggct gggactacga
     1381 cccgtacatt tcccacgagt tgggcgagct ggcggtggag acgggggtct ggcccctctt
     1441 cgaggtggag gacggtatcc tgaagttcta cgggaagtcc aagggtatcg cccaaggacg
     1501 gaagcgcaag cccgtgcgcg agtacctcct gaagcagggg cgcttcgccc acttcctcga
     1561 cgaggacatc gaatacttcc agaagaagat cgaccagatg tgggaccgct ggctggttcc
     1621 gggtgtctgc gccttcgaca tggccgagga cctgaagaag gccctggaca agtcggaggc
     1681 tgagctcgag gccgtcgagt agggactgat cagcaaccgg ggcatcccag ggggtgcccc
     1741 ggttgctgac ggttcccgcc tccggaagag cggttctttc catcacagga aggccgtgca
     1801 ttcagcacgg ggagaagagg ggattcccct tgcgattctc gtcgtgccca ctagtctgcc
     1861 cgggcgatcg ttcgcccttt ccgtccgtct gcccgatctc caccagatcg ccatcagcct
     1921 gtcggtgctc acgcgattcg ctcggagccg tcccgctcgc ggggagttgc cgtggaacac
     1981 tttagcatcg tggagaatta cagcacaggg ggagggggct ccatatgaac aacgcggcgc
     2041 aaaacaaggc agttctggtc aatcgctggt ggcagttggc ggcctgcatc gtggccatga
     2101 tggccatcgc caacctgcaa tacgcctgga cactctttac catcccgctc accaagagcc
     2161 tgcacgtcaa gctgtcggcc gtccagatcg cgttcacgct cttcatcctc accgagacct
     2221 ggctggttcc cttcgaaggc tacctcgtgg atcgcctggg agcacggctc gtggtgacgg
     2281 tgggaggcct gctggtgggg ataagctggg tgtggtccgg gctggcccaa acactccact
     2341 ctttgtatct cgcgtacgcc ctcggaggca tcggagccgg agcggtgtac ggcgcctgcg
     2401 tggggaccgc cctgaagtgg ttccccgacc gacggggcct tgccgccggc gccacagccg
     2461 gcgcctacgg catcggcacg gccctgactg tcatccccat caaccggatg atcgccggca
     2521 gcggctacca gcacgcgttc atcgtgtggg gggtg
//
LOCUS       DBNGZC010001014         1471 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2435412, whole genome shotgun sequence.
ACCESSION   DBNGZC010001014 DBNGZC010000000
VERSION     DBNGZC010001014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1471)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2435412"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="AC16G0_17485"
     CDS             complement(<1..255)
                     /locus_tag="AC16G0_17485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101450.1"
                     /translation="MQRKALRRFGQQSGGFILVLALAFAALLGVASVLAIGPSVGEAL
                     RSGQELRDKRLLFRADGGATLCRAELRNRLNSMLATKLAAI"
     gene            939..>1471
                     /locus_tag="AC16G0_17490"
     CDS             939..>1471
                     /locus_tag="AC16G0_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HIR5101451.1"
                     /translation="MNTFVERHAETIRGVLSCFDRVVITGTLPDLCHAGAMAQYLGAR
                     DIRLFDYPRWAEPFRDELRAHAERLAQESRLTIEFIRRGKDFRKEERIKRLVAQRGEH
                     PGLVHIFSAMEPCPSFRPWHDKRSGKTGLKPTEAKCLHYYFYFIDPALGLCYLRVPTW
                     APFRLQFYCNGHHVLAC"
BASE COUNT          259 a          515 c          431 g          266 t
ORIGIN      
        1 gatggccgcc agttttgtcg ccagcatcga attcagccgg ttcctcagct cggcccggca
       61 gagtgttgcc cccccgtcgg cccggaaaag gagccgcttg tcgcgcagtt cctggcccga
      121 tcgcagggcc tcccccacgc tggggccgat ggccagcaca ctggcgacgc ccagcagggc
      181 cgcgaaggcc agggccagga cgaggatgaa gccccctgac tgctggccga accggcgtag
      241 cgccttgcgc tgcatgtcac cctccaccgc cgactaggag gctgtcggag caacatcctc
      301 cggcttattt ggacgagtct tcaacctcta tcatctatga aatgatggat cgagccgcga
      361 tcggcgatcc agagccaaag atgatggtcc cttttcttgt gtgcgatacc taacccaacc
      421 actccgtcag accccgagag tccagacccc ccaacccgcc gatccccagg aaggccggtg
      481 cgtcacctaa ccacagaacg agacgagtct tgccgggatt atgtctggtt cccagacgac
      541 agccccgaga catgtacctc ggatggccta tccaccaccc tcgacgcccc tgtcggattg
      601 attccctatg gcctctctgc acccaaacca agccggccca ccgaggagtc cgtcctccag
      661 tgggccgtgg gttagcctgg cagcccgagg ggctgccgat tcgcaatgtc cctcagacga
      721 ggtcacccgc ttgccctcct gcgtgggtca cgggaaaccg gcgacgctgg gcaaggttac
      781 cttgaaggtg ttggctgtgt caagggtgag gccgggtggt tgtctttgct cagagaattt
      841 gtcctcccct aacccggcat agccggcacc aaacagggca tgagatgcgg atctatccgt
      901 ggtgagtatt gggtcggtgc gctacactga tcagcgttat gaacaccttc gttgaacgac
      961 acgccgagac gattcgcggg gtcctctcct gctttgaccg ggtggtgatc accgggaccc
     1021 tccccgacct ctgccacgcg ggggccatgg cccagtacct cggggcgcgg gacattcggc
     1081 tcttcgacta cccccggtgg gccgagccgt tccgcgatga gctccgggcc cacgccgagc
     1141 ggctcgcgca ggagtcccgg ctcacgatcg agttcatccg ccggggcaag gacttccgca
     1201 aggaagaacg gatcaagcgc ctcgtcgccc agcgcgggga gcaccccggt ctcgtccaca
     1261 tcttctcggc gatggagccc tgcccctcgt tccggccctg gcacgacaag cggagtggga
     1321 agacgggcct caagcccacc gaggccaagt gcctccacta ctacttctac ttcatcgacc
     1381 cggccctcgg gctctgctac ctccgggtgc cgacctgggc cccgttccgc ctccagtttt
     1441 actgcaacgg ccaccacgtc ctcgcttgcc a
//
LOCUS       DBNGZC010001015         3642 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1748823, whole genome shotgun sequence.
ACCESSION   DBNGZC010001015 DBNGZC010000000
VERSION     DBNGZC010001015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3642)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3642)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3642
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1748823"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="AC16G0_17495"
     CDS             complement(<1..185)
                     /locus_tag="AC16G0_17495"
                     /inference="COORDINATES: protein motif:HMM:NF040043.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HIR5101452.1"
                     /translation="MQDLSRDLLRGAVDLHIHAAPDIVPRLLDDLALAKAARDAGMAG
                     IVLKSHHMLTADRAQIA"
     gene            complement(361..1020)
                     /locus_tag="AC16G0_17500"
     CDS             complement(361..1020)
                     /locus_tag="AC16G0_17500"
                     /inference="COORDINATES: protein motif:HMM:NF013353.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HIR5101453.1"
                     /translation="MPPRIALIHAVTTAIPPTLHAFKEGWPEARLSNLLDDDLMPAYT
                     REGGLTPHIVERICELALYAARTGADGILFTCSVFPQAEEMAKQLVRVPLLKPDEAMI
                     AAALEKGKRIGVVVTNPPAGPAAEVQLRAAAAVQGRDIEIVQSLAEGAFAIGNTGDAV
                     THDRMVIEAAERIADQVDVLCLAQVSMALARAAVQSRVKVPVLTSPATAVARLKRLFN
                     G"
     gene            complement(1112..2086)
                     /locus_tag="AC16G0_17505"
     CDS             complement(1112..2086)
                     /locus_tag="AC16G0_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012694954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HIR5101454.1"
                     /translation="MKRLVAITAILLLSVAAVAVAPAADYPAKGKSITMILGTSAGGS
                     TDVGARILAAALEKDLVATGTRIQVENKPGAGWQIGLTALARSRPDGYTIGFTILPQT
                     ITNYLDPERKAVFNRKSFQPLGMQVVDPGVIAVKASSPYKTLKDVIEAAKATPGKLKA
                     SATGILSDDHLAVLQLEKLTGAKFAIVQFDGSAKAMTALLGGHTDVYFGNIGDTFPQA
                     KAGEVRVLCVMDDQESKFLPGKTSFSQGIKLESSSSRGLSAPTGTPKDVVDFLAGAIK
                     KAIDNPDHMKKMEEQGLTVRYMDPAGMEKYWSDMEEQIRPLMSIAKEK"
     gene            complement(2150..3634)
                     /locus_tag="AC16G0_17510"
     CDS             complement(2150..3634)
                     /locus_tag="AC16G0_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR5101455.1"
                     /translation="MLGFSVALTPTNLLFAFAGSLFGTIVGILPGLGPAAGTALLIPL
                     TFRLDPTGAIIMLAAIYYGSQYGGTITSVLMNVPGEAASAITCLDGYQMAKQGRAGAA
                     LSIAAIGSFIGGTLATLGLVVAAQPLTRVALRFGPVEFFALTVLGIVLVMGLAGKSMV
                     KALIMAVFGLLIGMVGMDPTRGSPRFTFGLVELLDGVGFIPVIMGLFGVAEIFENLES
                     DDQHVIEAQLSTLVPSAADLKASGWAIGRGTVVGFLLGLIPGMTGTASSVLAYVAERK
                     ASRYPEKFGTGVIEGVAGPETANNAHANAALIPLFTMGIPASPSIAVLMGAFMMNGLI
                     PGPLLFREHAALAWGVIASLYIGNVILLVLNLPLIPLWVKFLKIPYTILMTIVLAIMM
                     VGAYTVSNTVFDIFVMLLFGIIGYVLRKLDFPLAPIVLTLIMGPPMEKSLRQSLEMSQ
                     GDVSILVTSPIAASLLAISAFIMFSPMLKFAWKRWIVQPTAQRV"
BASE COUNT          688 a         1153 c         1175 g          626 t
ORIGIN      
        1 tgagcgatct gggcccgatc ggcggtcagc atgtggtggg acttcaacac gatccctgcc
       61 atgccggcgt cgcgggcggc cttggctagc gcaaggtcgt ccagcagccg ggggacgatg
      121 tccggggccg cgtggatatg gagatccacg gcaccacgca gcagatcacg ggagagatcc
      181 tgcattttac accctctttc ggatgccgac gggaaatcgc cgggacagcg agcccagacc
      241 cgctccgtct cgggccgtca agttgtccgt agtcatcacc ggaaagcttc ttcgcctggg
      301 gataatacgc caaaggcgtc agcagcgtca aagccggacc gggagccgac ttgctcgccg
      361 tcacccgttg aagaggcgtt tcaggcgggc gaccgccgtg gcggggctgg tcaggaccgg
      421 aacctttacc ctggattgga cggcggccct agccagagcc atggacacct gggcaaggca
      481 gaggacgtcc acctggtccg cgatgcgctc tgcagcttcg atcaccatcc gatcatgggt
      541 gacggcatcc cccgtgttcc cgatggcgaa agcgccttct gccagagact gcacgatctc
      601 gatgtcccga ccttggacgg cagcggccgc ccggagctgc acctctgccg caggacccgc
      661 cgggggattg gtcaccacca cgccgatccg tttccccttc tccagcgcgg ccgcgatcat
      721 cgcctcgtcc ggcttgagca gcggcacccg gacgagctgc tttgccatct cctccgcctg
      781 agggaacacg gaacacgtga aaaggatccc gtcggccccc gtccgggcgg cgtacagggc
      841 gagctcacag atccgctcga cgatgtgggg tgtcagccct ccctcgcggg tgtaggcagg
      901 catcagatcg tcatccaaca ggttgctgag cctggcctcg ggccatccct ccttgaaggc
      961 gtgaagggtg ggtgggatgg ccgtggtgac ggcatggatt agtgcgatgc gcggaggcat
     1021 gggtggctcc cgcccggtgg cagcgatgat cgacagcgac ggggcgagcc ggtgaccggg
     1081 cacgcccctt gtcgctgttc ctgttgagtg cttacttctc cttggctatg ctcatcaggg
     1141 gcctgatctg ctcttccata tcgctccagt acttctccat cccggcggga tccatatagc
     1201 ggacggtcaa cccctgctcc tccatcttct tcatgtgatc gggattgtcg atggccttct
     1261 tgatcgcgcc cgcgaggaag tccacgacgt ccttgggcgt gccggtcggg gcggacaggc
     1321 cgcgcgagga cgacgactcc agcttgatgc cttgcgagaa gctggttttc cccggcagga
     1381 acttgctctc ctggtcatcc atcacacaga gcacacgcac ctcgcctgcc ttggcttggg
     1441 ggaaggtgtc gccgatattc ccaaagtaga cgtccgtgtg gccgccaagc agggcggtca
     1501 tcgccttggc cgacccgtcg aactgcacga tggcaaactt tgccccggtc agcttctcga
     1561 gctggagaac ggccagatgg tcatcgctga gaattcccgt cgccgaagcc ttgagcttgc
     1621 cgggagtggc cttggccgcc tcgatgacgt cctttaaagt cttgtacgga ctgctggcct
     1681 tcaccgcgat tacgccggga tccaccacct gcatcccgag gggctggaag gatttccgat
     1741 tgaagacggc ctttcgctcc ggatccaggt aattcgtgat cgtctgcggc agaatggtga
     1801 acccgatggt gtagccgtca ggccgggacc gcgccagcgc ggtgagaccg atctgccacc
     1861 cagcgccggg cttgttctcg acctggatgc gcgttcccgt tgcgaccaag tccttttcca
     1921 gagccgcggc caggatgcgt gcgccgacat ccgtggaccc gccggcactc gtgccgagga
     1981 tcatggtgat ggacttcccc tttgcggggt aatccgccgc aggggcgacg gcgaccgcag
     2041 caaccgacag gagcaggatg gcggtgatgg ctacgagacg cttcatttcc ggtcctcctt
     2101 tcgccgggaa tggagccacc ggcagcggac agcaggatcg cccgaagatc tacacccgct
     2161 gggcagtagg ctggactatc cagcgcttcc acgcaaactt tagcatcggg ctgaacatga
     2221 tgaacgccga tatggccaac aggctggccg caatcgggct ggtaaccaag atggagacat
     2281 ccccctggga catctccagg gactggcgaa gggacttttc catgggcggc cccatgatga
     2341 gggtgaggac gatgggggcc agcgggaaat ccagcttgcg cagcacgtac ccgatgattc
     2401 cgaagaggag catgacgaag atgtcgaaga cggtattgct cacggtgtat gcgccaacca
     2461 tcatgatcgc caggacgatg gtcatcaaga tcgtgtaagg aatcttgagg aacttcaccc
     2521 agaggggaat gaggggaagg ttgagaacga gcaggattac gtttccgatg tacaggcttg
     2581 cgatgacccc ccaggccagg gcggcgtgct ctctgaaaag gagcgggccc ggaatgagcc
     2641 cgttcatcat gaaggcaccc atgaggacgg cgatggaggg cgaggcggga atccccatgg
     2701 tgaagagcgg gatgagtgca gcgttggcat gcgcattgtt cgccgtctcc ggcccagcca
     2761 ccccctcgat gacgcccgtc ccgaacttct ccggatacct tgatgccttg cgctcggcca
     2821 cgtaggccag cacggaactc gccgtccccg tcatccccgg gatcaacccc agcaggaacc
     2881 ccaccaccgt cccgcggccg atggcccagc cggaggcctt gaggtccgca gcgctcggca
     2941 caagcgtgct gagctgcgct tcgatgacat gctggtcgtc ggactccagg ttctcgaaga
     3001 tctccgccac cccgaacagg cccatgataa ccgggatgaa gcccaccccg tcgaggagct
     3061 cgaccaggcc gaaggtgaac cggggggagc cccgcgtggg gtccataccc accatgccga
     3121 tcaagaggcc gaagaccgcc atgatgagcg ccttgaccat ggatttgccg gccagcccca
     3181 tcaccagcac gatgcccagg acggtgaggg cgaagaactc gaccgggcca aagcgcaagg
     3241 cgaccctcgt cagcggctgg gcggcgacca ccaaaccaag ggtggccagc gtgcctccga
     3301 tgaacgaacc gatggccgcg atgctgagcg ccgcccctgc ccgaccctgc ttggccatct
     3361 ggtagccgtc gaggcaggtg atggcggagg cggcctcccc cggaacgttc atcaacacgg
     3421 aggtgatcgt cccgccgtac tgcgagccat agtagatcgc cgccagcatg atgatggccc
     3481 cggtcggatc cagacggaag gtcaggggaa tcagcagggc ggtcccggcg gccggaccca
     3541 gacccggcag gatgccgaca atcgtcccga agagggagcc cgcgaaggcg aataggagat
     3601 ttgtcggtgt gagggccacc gagaatccaa gcatcaggct gc
//
LOCUS       DBNGZC010001016         1775 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1850793, whole genome shotgun sequence.
ACCESSION   DBNGZC010001016 DBNGZC010000000
VERSION     DBNGZC010001016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1850793"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="AC16G0_17515"
     CDS             complement(<1..694)
                     /locus_tag="AC16G0_17515"
                     /inference="COORDINATES: protein motif:HMM:NF024776.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5101456.1"
                     /translation="MRPITFTAEDRQALAHDRYYHPDPRVQRKMEVLWLKSHGLTHDQ
                     IATYADVSRSTVQRYLDQYLAGGLQRLRQCQWHQPQSALAEHQDSLEEYFRKQPVRSA
                     KQARAIIEQRTGLRRSLSQVRHFLKGRLGLRWRKTGAIPVPPKKTVEEHAREQAAFVQ
                     EKLEPRLEQARQGQRQLFCVDAAHFVFAPFLGCLWCAARLFVRAASGRKRFNVLGALD
                     AVTHRLIRVTNHD"
     gene            complement(1310..1576)
                     /locus_tag="AC16G0_17520"
     CDS             complement(1310..1576)
                     /locus_tag="AC16G0_17520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101457.1"
                     /translation="MTHATRESKSRGVAPGEHKEGTITKTIEHYTSMVPSGTYLTLAI
                     GSIGLAAAMKLMGRDKDAQFIGHWVPTILILGLYNKLVKVQGSD"
BASE COUNT          298 a          576 c          584 g          317 t
ORIGIN      
        1 agtcgtgatt ggtcacccgg atcagccggt gcgtcaccgc gtccagcgcc cccaggacgt
       61 tgaatcgctt gcgacccgag gccgcccgca cgaacagccg cgccgcgcac cacaagcaac
      121 ccaggaacgg cgcgaacacg aagtgggcgg cgtcgacgca gaacagttgc ctctggccct
      181 ggcgggcctg ctccaggcgc ggttccaact tctcctgcac gaaggcagcc tgctctcggg
      241 cgtgctcctc gaccgtcttc ttgggaggga cggggatcgc cccggtcttg cgccaacgca
      301 gccccagccg gcccttgagg aagtggcgga cctggctgag gctcctgcga aggccggtcc
      361 gctgctcgat gatagcccgg gcctgcttgg cggagcggac cggttgcttc cggaagtact
      421 cctccaggga gtcctggtgc tcggccagtg cgctctgggg ctgatgccat tgacattgac
      481 gcagacgctg cagtccgccc gcgaggtatt ggtcgaggta acgttggacg gtgcttcggg
      541 agacgtcggc ataggtggcg atctggtcgt gggtcaggcc gtggctcttg agccagagga
      601 cttccatctt gcgctggacg cggggatcgg ggtggtagta ccggtcgtgg gccagggcct
      661 gccggtcttc cgccgtgaag gtgatcggcc gcatgacggg tccactcgat gctcctcaag
      721 tcgctccccg tcagcagttt aaacccacgc acggccgacg acaatttgtg tcggacctgg
      781 ccgcgcggag tatataacac gactttgtca gtcaggcagg gaactgctct ggcgcgctca
      841 taagctcacg cgacagcaac gccttgaccg cttcgaggcg gagccagtcg cagacctggc
      901 gatgcacctc cggcagcgac caccgacacc ggtccggccg agggggaaaa agctcgccgc
      961 gggcgccccg ggcgggctcg tccttgccgg ccttgccacc cctgccgggc caggaagctg
     1021 tacgccagca tcacgctcac tgcgtgccgg tggaagcccc tgatggttgc cgtctccggc
     1081 ttcgggcacg aatcagatcg tcgccgctcg catgaagccg gccagccgtt cggtcagcgt
     1141 agggcaccac cgaatttccg ggccacatcc gcgatgaacc tcgcgtagcc gccggggagc
     1201 cgagggtcgc tcggcaggaa acggctcatg aaggatcaga cgcctctcgc cggacctctc
     1261 gggcctcggc acggaccagg gctgcttgcg gctcccgttc tcgaccgggt cagtccgatc
     1321 cctggacctt gacgagcttg ttgtacagtc ccaggatcag gatcgtcggc acccaatggc
     1381 cgatgaactg ggcgtccttg tcccggccca tgagcttcat ggcggccgcc aggccgatcg
     1441 agccgatcgc aagagtgagg tatgtccctg agggtaccat gctcgtgtag tgctctatcg
     1501 tcttggtgat cgtgccctcc ttgtgctccc cgggggcgac gcccctactc ttactctcac
     1561 gcgtggcatg tgtcatgttg cgactcctca tgaaaagaga accgagaatc aagtcaatgg
     1621 tgacagaaat cgagcaattg gttgaccgat cttgtgcttc gcctgtacgc cgaggatcgg
     1681 ccacgcagga attccgctgc ccacgtcgat gagctgaggt cgccggtggg gcgcacacct
     1741 cggtgtggcg agccccggaa caggaaacgc ggaag
//
LOCUS       DBNGZC010001017         3328 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2143640, whole genome shotgun sequence.
ACCESSION   DBNGZC010001017 DBNGZC010000000
VERSION     DBNGZC010001017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3328)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3328)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3328
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2143640"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="AC16G0_17525"
     CDS             <1..895
                     /locus_tag="AC16G0_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HIR5101458.1"
                     /translation="TATATTDAHEWKYPYSVGETAYAWVPWGILIVCCAIWGMPEWRV
                     YLNNLLKGITFKTTLFGSAFAGSLSLPTWQMPALHSLVQRMPPVAPVNAKPEAAVFVI
                     NYLSASGTGVFVAALLSGLALGLSPTQWGEAVRRTARRLVIPILVIGQVLGLGFLTRY
                     SGTDAVLGLAFTSAGPLYPFFAAYLGWLGVFLTGSDTASNALFGSLQRITAQQLHLNE
                     ILIVATNSTGGVMGKMIDAQSIMVACAACYDDPYERTHALGPIFRTVFWHSVAGAAVI
                     GLIAFLQAYVFTGIIPIPPIK"
     gene            1007..1747
                     /locus_tag="AC16G0_17530"
     CDS             1007..1747
                     /locus_tag="AC16G0_17530"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017721486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR5101459.1"
                     /translation="MAVKAQLFITCLAEQFYPNVLKGMVALLERLGVQVAFPQEQTCC
                     GQPFFNSGYRTQARELAVKWLEVFGRSEGFIVSPSGSCVDMVRHHYPNLFPAGTLEHR
                     QAEEVASRTFEFTQFLVNVLKVTDVGARFPQKVTYHASCHLLRGLSAREEPKMLLRGV
                     KGLELIPLSNEETCCGFGGVFSVIYPEVSQAMMEAKIRAIEASGAQAVVACDAGCLMN
                     IAGGLRKAHSPIKAMHLIEVLTADRGTP"
     gene            1744..3174
                     /locus_tag="AC16G0_17535"
     CDS             1744..3174
                     /locus_tag="AC16G0_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017721485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HIR5101460.1"
                     /translation="MSQATVPIDFRTNAQHVPPKMPAAVQKATAKFLGTRSALVEALG
                     NDLWQTLRQAGHDVRLHAITHLDHYLEQAERAVTKAGGHVHWARDAGEANRIVLEIAR
                     QHHVKKVVKSKSMATEEIGLNHAMEARGIQIIETDLGEYIVQLLGVGPAHIVVPAFHL
                     SKEEIAALFREKLGVDAPAEAEKLTQVARTKLREEFLTADLGISGGNFLVAETGTLVT
                     VTNEGNGRMCTTIPPVHVAIVGIDKIVPDWESLTVLLKVLARSATGQKITCYTQFISG
                     PPRADAETGPKEFHLVLLDNGRSRILQSPETRETLLCIRCGACLNICPVFNNVGGYTY
                     GYPISGPTGVLFASQILGVKAAGGLPFASTLCGACNDICPVKIPITEILLHLRHRVVE
                     GDEVEKPAAPYHLRVGAQVGSVALGTPWLYRVGSQILKRIQAPFVRDGWMASLPAPLN
                     RWTMSRPFPAFAPGFRRWWRGRSERG"
BASE COUNT          578 a         1054 c         1085 g          611 t
ORIGIN      
        1 gacggctacg gcgaccactg acgctcatga gtggaagtat ccgtactcag tcggcgagac
       61 ggcctacgcc tgggtgccgt gggggatcct tatcgtctgt tgcgccattt ggggcatgcc
      121 ggaatggcgg gtctacctga acaatctgct caagggcatc accttcaaga ccaccctctt
      181 cggctccgcg ttcgccggct cactctccct tccgacgtgg cagatgccgg cgctccatag
      241 tctggtccag cgcatgcccc ccgtggcgcc ggtcaatgcg aagccggagg cggcagtgtt
      301 cgtcatcaac tatctgtcgg cgtcagggac cggcgtgttc gtagccgcgc tgctgtccgg
      361 cctggctctg gggttgagcc ccacccagtg gggcgaagcc gtccgccgca ccgcaagacg
      421 cttggtgatc cccatcctgg tcatcgggca ggtgctgggg ctcgggttcc tcacgcggta
      481 ctctgggacg gatgctgtcc tgggactggc gttcaccagt gcaggtcccc tctacccatt
      541 cttcgcggcg tacctcggct ggctgggggt gttcctcacc ggctccgata cggcgtccaa
      601 cgccctgttc gggagcctcc agcggatcac cgcgcagcaa ctgcatctga acgagatcct
      661 gatcgtcgcg accaactcca ccggtggcgt catgggcaag atgatcgacg cccagtcgat
      721 catggtggct tgcgccgcct gctacgacga tccctacgag cggacccatg ccctggggcc
      781 gatcttccgt acggtgttct ggcattcggt cgccggagcg gccgtgatcg ggctgatcgc
      841 ttttctgcag gcctatgtgt ttactgggat cattccgata ccacccatca agtgagtggc
      901 tgctcggttc gagacggggg gcgggtcgcc gcccccgtcc gtttctggta tgctcccaac
      961 ttttgcagac cggagaggac atcgtcacta gacggaaggg atccgcatgg ccgtcaaggc
     1021 gcaacttttc atcacctgcc tcgccgagca gttctatccg aatgtcctga aaggcatggt
     1081 cgcgctcctg gaacggctgg gggtccaggt ggccttcccc caggagcaga cgtgttgcgg
     1141 gcagcccttc ttcaatagcg gctaccggac tcaggcgagg gaactcgccg tcaaatggct
     1201 ggaggtcttc ggccggagcg agggcttcat cgtttcgccc tccggctcgt gcgtggacat
     1261 ggtccggcac cactatccca acctgttccc tgccgggacg ctggagcacc ggcaggcgga
     1321 ggaggtggcc tcccggacct tcgagttcac ccagtttctg gtcaacgtcc tgaaggtcac
     1381 ggacgtgggg gcacgcttcc cccaaaaggt gacgtaccat gcatcctgcc acctcctccg
     1441 cgggctctcg gctcgcgagg agcccaagat gcttctccgg ggagtcaagg ggctggaact
     1501 gatcccgctg tcgaatgagg agacctgctg cggcttcggc ggagtcttca gcgtgatcta
     1561 tccggaggtg agccaggcca tgatggaggc caagattcgc gccatcgagg ccagcggggc
     1621 ccaggctgtg gtcgcctgcg atgccgggtg tctcatgaac atcgccggcg ggttgcgcaa
     1681 ggcgcactcg ccgatcaagg ccatgcatct gatcgaagtg ctgacagcgg atcgaggtac
     1741 gccatgagcc aggccaccgt tcccatagac tttcggacca atgcgcaaca cgtccctccg
     1801 aagatgcccg cggcggtgca gaaggctaca gctaagttcc tgggcactcg gtcggccctg
     1861 gtagaggccc tcggcaacga cctgtggcaa acgctgcgcc aggcaggcca cgatgtccgc
     1921 ctacacgcca tcacacacct cgaccactat ctcgaacaag ccgaacgagc ggtgaccaag
     1981 gcgggggggc acgtccactg ggcgcgggac gcaggggagg ctaatcggat cgtcttggag
     2041 atcgctcgcc agcaccacgt gaagaaggtg gtgaaatcga agagcatggc caccgaggag
     2101 atcggcctca accatgctat ggaggcgagg ggcatccaga tcatcgagac cgacctaggc
     2161 gagtacatcg tccaactcct cggcgtcggg ccggcgcaca tcgtcgtgcc ggcgttccac
     2221 ctgtccaagg aagagatcgc cgccctgttc cgagaaaagc tcggagtgga cgcaccggcg
     2281 gaggcggaga agcttaccca agtcgcgcgc acgaagcttc gcgaggaatt tctgaccgcc
     2341 gacctgggca tctccggggg caatttcctc gtggccgaga ccgggaccct cgtcacggtg
     2401 acgaacgagg ggaacggacg gatgtgcacg accatccccc ccgtgcatgt ggccattgtc
     2461 ggaattgaca agatcgtgcc ggattgggag agcctcaccg tcctcctgaa ggtgctggcg
     2521 cggagcgcca ccgggcagaa gatcacctgc tacacccagt tcatcagcgg cccaccgcga
     2581 gcggatgcag agaccggacc caaagagttc catctcgtcc tcctggacaa cgggcgctcc
     2641 cgtatcctgc agtcccccga gacccgcgag actctcctct gcatccgttg cggagcctgt
     2701 ctcaacatct gtcccgtctt caacaacgta ggggggtaca cgtacgggta tccgatctcc
     2761 ggacccacgg gggtgttatt cgcaagccag atcctcggcg tgaaggccgc gggaggactg
     2821 ccgttcgcct cgacgttgtg cggtgcctgc aacgacatct gcccggtgaa gattcccatc
     2881 acggagatcc tgctccactt gcgccatcgg gtggtcgagg gagatgaagt ggagaaaccg
     2941 gctgcgccct accatctccg ggtgggggcg caggtgggct ccgttgcgct ggggacgcca
     3001 tggctgtaca gggtgggctc ccagattctg aagcgaatac aggcgccgtt cgtgcgcgac
     3061 ggctggatgg ccagtttgcc ggcccccctg aatcgatgga ccatgtcgcg gccattcccc
     3121 gcctttgccc ccggtttccg gcggtggtgg cggggtcgct cggaaagggg ctaggcggtc
     3181 aggggaaggc ttggaggatg tcatggattt ggcgcgcgaa gacattctag ggaaagtacg
     3241 aaaggcgttg ggcgggcggc cgggctcaca gcctgccggc aggggggtgc cggtcctcgc
     3301 cgtcccggct gcggccaggg ttgcccct
//
LOCUS       DBNGZC010001018         2142 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2143734, whole genome shotgun sequence.
ACCESSION   DBNGZC010001018 DBNGZC010000000
VERSION     DBNGZC010001018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2143734"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /locus_tag="AC16G0_17540"
     CDS             complement(<1..743)
                     /locus_tag="AC16G0_17540"
                     /inference="COORDINATES: protein motif:HMM:NF015410.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HIR5101461.1"
                     /translation="MAAAVRALSRLFRWGAPPPALGTTYSPETEVSLRFRGLLEAEDP
                     VPSAGDGEALRRALLPAVKAFIATERDEIEAAHRGGASGLEVVARHADLVDAVVCELF
                     RLADEGAPRAVRERTEGCAVVALGGYGRRELNPASDVDVMFLYPRRVDGYVTAMLDHV
                     LYFLWDLGFEVGHSCRSLSDTVQMLDADLTVRTALLEARFLAGSYGVYAGLQERMWRA
                     FQGRRAQQYVQHKLIEQERRHAKHGGSVYV"
     gene            complement(915..1328)
                     /locus_tag="AC16G0_17545"
     CDS             complement(915..1328)
                     /locus_tag="AC16G0_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020878603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR5101462.1"
                     /translation="MPRIAVDLIIELPEGRIVLIRRKNPPHGWALPGGFLEYGESLEQ
                     AAVREAKEETGLDVELIRQLHTYSEPARDPRGQTVSTVFIARSHGIPRAGDDAGEVRV
                     VSLTALPSPLAFDHERILADYRGSREGAPPDRDRP"
     gene            complement(1399..>2142)
                     /locus_tag="AC16G0_17550"
     CDS             complement(1399..>2142)
                     /locus_tag="AC16G0_17550"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012035746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HIR5101463.1"
                     /translation="PDGTELTAAETQRYARQMIIPNWGEATQRKLKASKIFIAGAGGL
                     GSPVSFNLAVAGIGLIRICDFDSPELSTLNRQFLHDDSRVGMNKAESAKRTLSRLNPH
                     VRIEAITEKIDEASVDRLVADSDLILDCMDNYPTRFILNAAAIRKGIPLIHGSIWGFE
                     GRVTFIQTPETACLACLFKESPPREVFPVLGATPGITGTIQAMEAIKYLAGVGSLLKG
                     RLLCCDFLEMRFFEVKVRKDPACAACGGA"
BASE COUNT          351 a          745 c          707 g          339 t
ORIGIN      
        1 acgtacaccg acccaccgtg cttggcatga cggcgctcct gctcgatgag cttgtgctga
       61 acgtactgct gggcccggcg cccctggaac gcccgccaca tccgctcctg gaggccggca
      121 tacaccccgt agctacccgc caagaaccgg gcctccagca gggcggtccg gaccgtaagg
      181 tccgcgtcca gcatctgcac cgtgtcgctc aaggagcggc aactgtgccc aacctcgaag
      241 cccaggtccc acaagaagta gaggacgtgg tccagcatgg cggtgacgta gccatccacg
      301 cgcctggggt acaggaacat cacatccacg tcggaggcgg ggttgagctc gcgccggccg
      361 taacccccca gggccacgac cgcgcaaccc tccgtccgct cgcggactgc ccggggtgcc
      421 ccctcgtcgg ccaagcggaa gagctcgcac accacggcat ccaccaggtc tgcatgccgg
      481 gccaccacct ccaggccact ggccccgccc cgatgggccg cctcgatttc atcgcgctcc
      541 gtggcgatga aggccttgac cgcggggagc aacgccctgc gcaaggcctc tccatctccc
      601 gcggagggca cgggatcctc ggcctccagc aggccacgga aacggaggct cacctccgtc
      661 tccgggctgt acgtggtccc caaggcgggc gggggtgcgc cccagcggaa gagcctgctc
      721 agggcgcgaa cagcagccgc catgttgcct ccattggcct gtcacctctg ctccgcacta
      781 tacccggccg gtcctgctcg ggtcaaggcg gccagcggac gcagagggca gacgaatccc
      841 tgccgggggc aggctgcggg ggcgcgtcac gacctctgac atgcaggcag ggcgggatcc
      901 ggtgggccgc ggcctcaggg gcgatcccgg tcaggagggg ctccctcccg ggacccccgg
      961 tagtctgcga ggatgcgctc gtggtcgaag gccaaggggg aaggaagagc cgtcagggag
     1021 accacgcgca cctcgccagc atcgtcgccg gcccggggaa tcccgtgcga tctggcgatg
     1081 aacaccgtgc tcaccgtttg gccccgcggg tcgcgggccg gttccgagta ggtatgcaac
     1141 tgccggatca gctcgacatc caggccggtc tcctccttgg cctcgcgcac ggccgcctgt
     1201 tccaggctct cgccgtattc caggaagccg cccggcagcg cccatccgtg aggcgggttc
     1261 ttgcggcgga tcaggacgat ccggccctcc gggagttcga tgatcaggtc caccgcgatc
     1321 cggggaatcc tcgaggggga ggcgtcggtc cactcctccg ggtcatggct atcggccgcc
     1381 cgcgcctccg gctgcacgtc aggccccccc gcaggcagcg cacgccggat ccttccgcac
     1441 ctttacctcg aagaagcgca tctccagaaa gtcgcagcag aggagccggc ccttgagcag
     1501 gctccccacc ccggccaggt acttgatcgc ctccatggcc tggatggtgc cggtgatgcc
     1561 cggggtggcc ccgaggacgg gaaacacctc tcgtggaggc gactccttga agaggcaggc
     1621 cagacaggcc gtctcgggcg tctggatgaa cgtcacccgc ccttcgaatc cccagatgct
     1681 cccatgaatg agggggatcc ctttgcgaat ggccgcggcg ttcaggatga agcgcgtggg
     1741 atagttatcc atgcagtcga ggatcaggtc cgagtcggcg accaagcgat ccacactcgc
     1801 ctcgtcgatc ttctccgtga tggcctcgat ccgaacgtgg gggttgagtc tggacagggt
     1861 ccgcttggcc gactcggcct tgttcatccc gacgcgcgag tcgtcgtgga ggaactgccg
     1921 gttgagagtg gagagctccg gggagtcgaa atcgcagatc cggatgaggc cgatgcctgc
     1981 aaccgccagg ttgaaggaca ccgggctccc cagaccgccg gcgcctgcga tgaagatctt
     2041 tgaagccttg agctttcgct gggtcgcctc tccccagttg gggatgatca tctgccgagc
     2101 gtaccgctgt gtctccgcgg ctgtgagttc agtcccgtct gg
//
LOCUS       DBNGZC010001019         3620 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2436051, whole genome shotgun sequence.
ACCESSION   DBNGZC010001019 DBNGZC010000000
VERSION     DBNGZC010001019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3620)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2436051"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..818)
                     /locus_tag="AC16G0_17555"
     CDS             complement(291..818)
                     /locus_tag="AC16G0_17555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101464.1"
                     /translation="MTRIFRIEDGRAVRERQRALPRGTVVDAWPDVFDPRTIWIGETT
                     KRMLEETGAPLPASAVVEDSRIGVFFPDEPHDQASLPPEESLRVRVLSGHGMAVVWYG
                     STEGKGMRPLPEPVSPEDAFFFLMRMGSRINHAWRLFRTREEAMEFMRRHFPDNAEAR
                     TWAEALPAASYKELL"
     gene            complement(938..1243)
                     /locus_tag="AC16G0_17560"
     CDS             complement(938..1243)
                     /locus_tag="AC16G0_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR5101465.1"
                     /translation="MGLVRVAGSSELLPGQGRVVRAEGLEIALFNVDGSFYGIGNGCR
                     HRGGPLGEGSLEGSVVTCPWHGWEYDVTTGVSRVNPAMRVPVYRTEVKGDDVFVELP"
     gene            complement(1284..2129)
                     /locus_tag="AC16G0_17565"
     CDS             complement(1284..2129)
                     /locus_tag="AC16G0_17565"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HIR5101466.1"
                     /translation="MSITVQDAFGQGFELGAPARRIVSLIPSITEILFSVGVGEQVVG
                     VTKFCTEPAEAVRGKPRIGGPKNPRREAILGLMPDLVIANVEENHQADVDALRAAEVP
                     VFVTYPRTVREGIQLIRDLGALVGKSSSADAIAGMSEEALTAIEQHTDGCERVRVFCP
                     IWRRPYMTINADTYMDSMLWTCGGENIFRDSPVRYPRVDLAEMARLRPDVVLLPDEPY
                     PFGQKHLEDFRGMADAPAILTGRLHFVDGKVLSWYGPRIAESLRTLRGILHAGSGRRR
                     DLVIR"
     gene            complement(2178..3620)
                     /locus_tag="AC16G0_17570"
     CDS             complement(2178..3620)
                     /locus_tag="AC16G0_17570"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006968637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIR5101467.1"
                     /translation="MPVERLDEHRWQIPARSKPGMKVPAIIFANEKLMEQIRHDLSLE
                     QAANAATLPGVVRAAYAMPDIHQGYGLPIGAVVATRTDGGVVTPGGVGYDINCGVRLL
                     RTDLTQDQVLPHIKDLVNLLFAEVPTGVGSHGKVRLTKANQTRPLVEGARWAVAEGMG
                     QAEDLLYTEGSGCLPGADPDAVSHKAYERGRDQAGTLGSGNHFLEVQVVEQIYDAQAA
                     NVLDLFPGQVTVLIHTGSRGFGHQVCTDYLASMDRAAKRYGIQLADRQLACAPFDSEE
                     AQAYLGAMRAAANYAWVNRQCLTHWTREVFQRIFKKSPASLGMSVVYDVAHNIAKVEE
                     HMVEGQSRPLIVHRKGATRAFPPGHPELPERYRSIGQPVLVPGDMGRASFVLVGTKGA
                     MEETFGSTCHGAGRLLSRAAAIRAGKGRSIERELADTYGVYARASGRDSMKEEMPEAY
                     KNVQDVVTVCHNAGISTMVAKLKPLGCIKG"
BASE COUNT          616 a         1274 c         1085 g          645 t
ORIGIN      
        1 ggtccgccgc caggcagcca tgaagggcgg tgccgccccg gcggggaccg ggtctgccaa
       61 gtccaggcat cggccagagg ccgcacccca ggctgagccg cctgccagcc cgccgccttc
      121 cccgaccgaa ggggaagcgc ctccgactcc gactgcgtag gcctcccggt ctgcgctttc
      181 ttggaatccg tgcggttttc ccatccccga gcccttcgcc tcgaggagag gtgtgacaca
      241 tccggggctc ggggatcaaa cgcaggtcct cgctagcgct cactgcccgc tcaaagcagt
      301 tctttgtagc ttgcggccgg aagggcctcg gcccaggtcc gagcctctgc gttgtccggg
      361 aagtgccggc gcataaactc catcgcctcc tccctggtgc ggaagagcct ccaggcatgg
      421 ttgatgcggc tccccatgcg catcaggaag aagaaggcgt cttcgggtga aaccggttcc
      481 ggcagggggc gcatcccctt cccctcggtc gagccatacc agaccaccgc catcccgtgc
      541 cccgagagaa cccgcacgcg gagagattcc tctggaggca gggaggcctg gtcgtgcggc
      601 tcgtctggga agaagacgcc gatgcgagag tcctcgacca cggcacttgc agggaggggg
      661 gctcccgtct cttccagcat ccgcttcgtc gtctcgccga tccagatagt gcgagggtcg
      721 aacacgtccg gccaggcgtc caccacggta ccccgcggca gcgcccgctg ccgctcccgg
      781 accgcccggc cgtcttcaat gcgaaagatt cgagtcatga tgcgctaccg cttctccttc
      841 cgccccagtc attggcgcgt cgtcgagccg cggaccaggc ttgcaaagac gctctgcgtt
      901 tcgcatctac cgcagagatt cggcgccgcc gttcccttca cggcagctca acgaagacgt
      961 cgtcgccttt cacctcggtt cgatagaccg gaacccgcat ggcagggttc acgcgactca
     1021 ccccagtggt gacgtcgtat tcccaaccgt gccaggggca tgtcaccacc gacccctcca
     1081 gcgagccctc gcccagcggt cctccgcggt gccggcagcc attaccgatc ccgtagaatg
     1141 acccatcgac gttgaacaac gcgatctcga ggccctccgc cctgacgact ctcccttgcc
     1201 caggaagaag ctctgaagag ccagccacgc gtaccagccc catggtcctc tccgacaggc
     1261 attcaaccgt cagaaatcac ccgtcatcga ataaccaagt cccgacgccg tcctgacccg
     1321 gcatgcagga tccctcgcaa tgttcggagg ctttccgcga tccgcgggcc ataccacgag
     1381 aggaccttgc catcgacgaa gtgcagacgc ccggtgagaa tggcgggcgc gtccgccatg
     1441 ccgcggaagt cttcaaggtg cttctgcccg aaaggatacg gctcgtccgg caggagcacc
     1501 acgtccggcc tcagcctggc catttctgcc agatcgactc tgggatatcg gaccggggaa
     1561 tctcgaaaaa tattctcgcc gccgcaagtc caaagcatgc tatccatgta cgtgtcggcg
     1621 ttgatcgtca tgtagggtcg ccgccaaatc gggcagaaga cgcgaactcg ctcgcatcca
     1681 tccgtgtgct gttcgatggc ggtcagggcc tcctcgctca ttcccgcgat ggcgtctgcg
     1741 gacgacgatt tcccgacgag ggcccccaga tcgcggatga gctggatccc ctcgcgaacc
     1801 gtccggggat aggtcacgaa aaccggcacc tcggcggccc gaagggcgtc cacgtccgcc
     1861 tggtgattct cctcgacgtt ggcaatgacg aggtccggca tcagaccgag gatggcttca
     1921 cgacgcggat tcttcggccc cccgattctt ggcttccccc ggaccgcctc ggctggttcg
     1981 gtgcagaact tcgtgacgcc gacaacctgc tcgcccacac ccacactgaa tagaatctca
     2041 gtgatgctgg ggatcaggct gacaatgcgc cgggccggcg cccccagctc gaacccctgc
     2101 ccgaaggcat cttgaaccgt gatgctcatt cctcgttccg caaattcgca atccgcaatt
     2161 cgaatttcga aatggcgtca acccttgatg cacccgaggg gcttgagctt ggccaccatc
     2221 gtggaaatcc cggcattgtg gcacaccgtc acgacgtcct ggacgttctt gtaggcttcg
     2281 ggcatctcct ctttcatcga gtcgcggccg ctcgccctgg cgtacacacc gtaggtgtct
     2341 gccagttcac gttcgatgga ccgacccttg ccggccctga tggcggcggc ccggctgagg
     2401 agccgcccgg ccccatggca ggtgctgccg aaggtctcct ccatcgcccc cttggtcccc
     2461 accagaacga acgaagcccg ccccatgtcg ccgggaacca ggaccggttg gccgatggac
     2521 cggtagcgct ccggcagctc ggggtgtccc ggtgggaagg cgcgggtcgc tcccttgcgg
     2581 tggacgatga ggggcctgga ctgcccctcg accatatgct cttccacctt cgcaatgttg
     2641 tgggcgacat catacaccac cgacattccg aggctggcgg gtgatttctt gaagatccgc
     2701 tggaagacct cccgtgtcca atgcgtgagg cactgccggt tgacccaggc gtagttggcc
     2761 gctgcccgca tggcgccgag gtacgcctgc gcctcttctg aatcgaaggg ggcgcacgcc
     2821 agctgtcggt cggcgagttg gatgccgtac ctctttgccg cccgatccat gctcgccagg
     2881 taatcggtgc agacctggtg cccgaagccg cgcgagccgg tgtgaatgag gacggtcacc
     2941 tggccgggga agagatccag gacgttggcc gcctgcgcat cgtagatctg ctccaccacc
     3001 tgcacttcca agaaatgatt ccccgaaccc aacgtccccg cctgatcgcg cccccgctcg
     3061 tacgccttgt ggctcacggc gtcagggtcc gccccaggaa gacagccgga gccctccgtg
     3121 tacaggagat cctccgcctg acccattccc tccgccaccg cccagcgtgc gccctctacc
     3181 aggggtcggg tctgattggc cttggtgagc cgaaccttcc cgtgcgagcc cacgcccgtc
     3241 ggaacctcgg caaagagcag gttcaccagg tctttgatgt gcggcaggac ctggtcttgc
     3301 gtgaggtcag ttcgcaggag gcgcaccccg cagttgatgt cataccccac gccccccggc
     3361 gtgacgacgc ccccatccgt ccgggtcgcc accaccgccc cgatgggcag gccgtagccc
     3421 tggtggatgt cgggcatggc ataggccgcc cggaccacgc cgggcagcgt ggccgcgttc
     3481 gccgcctgct ccagcgagag gtcatggcgg atctgctcca tcagcttctc gttggcgaag
     3541 atgatcgcgg gcaccttcat ccccggcttg ctgcgcgccg ggatctgcca gcgatgctcg
     3601 tcaagccgtt cgacaggaat
//
LOCUS       DBNGZC010001020         3073 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_772098, whole genome shotgun sequence.
ACCESSION   DBNGZC010001020 DBNGZC010000000
VERSION     DBNGZC010001020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_772098"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..455)
                     /locus_tag="AC16G0_17575"
     CDS             complement(183..455)
                     /locus_tag="AC16G0_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR5101468.1"
                     /translation="MTKADLVERAAKAAKVTKKAAEAVLDSFMEGVRDSLKKGKRVSL
                     VGFGTFSVGKRAARNGRNPQTGEAIKIKAAKVPRFKAGKTLKDAIR"
     gene            complement(606..1940)
                     /locus_tag="AC16G0_17580"
     CDS             complement(606..1940)
                     /locus_tag="AC16G0_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HIR5101469.1"
                     /translation="MSLELVPRESPPPRKAAPLADRMRPRSLEEFVGQAHLLGEGKIL
                     RVALEQGEVPSLILWGPPGTGKTSLAMLMAAHVKAAFIPFSAVTSGIKEIKEVIAEAD
                     RRLRGGGQRTVLFVDEIHRFNKSQQDAFLPHVEHGTITLIGATTENPSFEVIAPLLSR
                     AKVLVLHPLTDVELQRIVERALHDPERGLGRSGFQVPDEALAAIIRWANGDARAALNV
                     LELAARLAEVSPQPRVISLAVVQEAAQQRSLLYDKAGEEHYNLISALHKSLRGSDPDA
                     ALYWLARMLASGEDPLYIARRLVRFASEDIGNADPSALTLAVAAKEAYHFLGSPEGEL
                     ALAQCVTYLATAPKSNAVYTAFGEAQADVAAAPADPVPLHIRNAPTPLMGELGYGEGY
                     KYAHDYEGAYVGQDYLPERLRGRVYYRPTDRGLEVEIQKRLAAWRRKPTAKG"
     gene            complement(2177..2785)
                     /gene="pyrE"
                     /locus_tag="AC16G0_17585"
     CDS             complement(2177..2785)
                     /gene="pyrE"
                     /locus_tag="AC16G0_17585"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR5101470.1"
                     /translation="MLTDSEMRLVDDLFGIQALKFGEFRLKSGALSPFYVDLRVIISH
                     PRTLRGVALRILDVLRPLHFDRIAGIPYAGLPIATAVSLAGDLPMLYARKEIKEHGIR
                     RTIEGKFVAGDVVVMIDDVITDGASKFEAAEPLREAGLVVQDFVVFLDREQGGAERLR
                     QKGLGLHSALRISAVLDHLRQTGHLPADQHARCLEFIARVRF"
BASE COUNT          542 a          963 c         1006 g          562 t
ORIGIN      
        1 gaccggggac cggatgaaga ttctcctgcg gaggcggaag gatccggaga caacgaaggc
       61 ctcgggggtg accccgatcc cttgggttct gagaatccgt cgttccgaaa tccgcaggaa
      121 tctgcgtaat ctgcggaaga aacagagctg gctcttcctc cggtctgcgg ttggcgattg
      181 ggctagcgga tcgcgtcctt caaggtcttg ccggccttaa agcgggggac cttcgctgct
      241 ttgatcttga tggcctcacc cgtttgagga ttccggccgt tccgggcggc acgcttgccg
      301 accgagaagg tgccgaagcc gaccaggctc acccgcttcc ctttcttcaa tgagtcccgc
      361 acgccctcca tgaagctgtc cagtactgcc tccgctgcct tcttggtgac cttggccgcc
      421 ttggccgctc gctccacaag atctgctttc gtcatgcgtc gaccctcctc tcctgccttc
      481 gggaagaccc cgggtattga tgaagactga ctgcaaagac gccgcgacgg ttcttatctc
      541 aaaagtcacg gaggagtgtc aaggcaaaaa gcgaaagaac cgagccgcca tgtagccgcc
      601 ggtcactagc ccttcgccgt tggctttcga cgccaggccg ccaaccgctt ttggatctcc
      661 acctccagac cgcggtccgt ggggcggtag tagacccgtc cacggagccg ctcggggagg
      721 tagtcctgcc cgacgtaggc gccctcgtag tcatgggcgt acttgtaacc ctcgccgtag
      781 ccaagctctc ccatcagcgg cgtgggggca ttccggatgt ggaggggcac cgggtccgcc
      841 ggggccgcgg ccacatccgc ctgcgcctcg ccgaaggcgg tgtagacggc attggacttc
      901 ggcgccgtcg ccaggtaggt cacgcactgg gccagggcca attctccctc cgggctcccc
      961 aggaaatgat aagcctcctt ggccgcgaca gccaaggtca gggcagacgg atcggcgttc
     1021 ccgatgtcct ctgaggcgaa gcggaccaga cggcgggcga tatacagggg gtcctcgccg
     1081 gaggcaagca tgcgcgccaa ccagtacagg gcggcgtccg ggtcgctgcc ccggaggctc
     1141 ttgtgcagcg ccgagatgag gttgtagtgc tcctctcccg ccttgtcgta gagaaggctc
     1201 cgctgctgcg cggcctcctg cacgacggcc aggctgatga cgcggggctg gggggaaacc
     1261 tcggccaatc tggctgcgag ttccagcaca ttcagggcgg cccgcgcatc cccattcgcc
     1321 caccggatga tggcggccag cgcctcgtcc gggacctgga atccgctgcg gcccagcccc
     1381 cgctccgggt cgtggagcgc ccgttccacg atgcgctgca gctcgacgtc ggtcaaggga
     1441 tgcaggacca agaccttggc acgagacagg agcggggcga tcacctcgaa ggagggattc
     1501 tcggtggtgg ccccgatcag ggtgatggtg ccgtgctcga catgaggcag gaaggcatcc
     1561 tgctggctct tgttgaatcg gtggatttcg tcgacgaaca ggacggtgcg ctggcctcca
     1621 ccccgcaatc gtcgatcggc ctcggcgatg acctccttga tctccttgat gcccgaggtc
     1681 accgccgaga acgggatgaa ggcggccttg acgtgggccg ccatgagcat ggcgagagac
     1741 gtcttcccgg tcccgggggg tccccacagg atcagggacg ggacctcgcc ctgctcgagg
     1801 gccacccgga ggatcttgcc ctcgcccagg aggtgcgcct ggccgacgaa ctcctccaga
     1861 ctgcgcggcc gcatgcggtc ggccaagggc gccgccttcc gcggtggagg agattcgcgc
     1921 gggaccagtt cgaggctcat gagcgagtcc gggttccagg ttcgatgttc aaggttcaag
     1981 gttgaacttc gaaccttgaa cggagaacgg ttgccacatc tggcagaatg cggaaaatct
     2041 cgttttcacc cagactgctc aaaaaggtcc agatgcaagg cggcgagcgc agggccgagt
     2101 gaggcgtact taagggtacg ccgcagcgag cgcccgagcg agacaacgcc gcagatgggc
     2161 ctttttcagc agtctgctag aagcggaccc tggcgatgaa ctccaggcat cgggcgtgct
     2221 ggtccgcggg cagatggccg gtctgccgaa ggtggtccag cacggcggag atccggaggg
     2281 cgctgtgcag gccgaggccc ttctggcgca gccgctcggc cccgccctgc tcccggtcca
     2341 gaaatacgac gaagtcctgg accaccaggc cggcttcccg aagcggttcg gccgcctcga
     2401 acttgctggc gccgtcggtg atcacgtcgt cgatcatgac aaccacgtca cccgccacga
     2461 acttgccctc gatggtccga cggatgccgt gctccttgat ctctttcctg gcgtagagca
     2521 tgggaaggtc tcccgccagc gaaacggcgg tggcgatggg aagtccggcg tacgggatgc
     2581 cggcgatgcg gtcgaaatgg agcggccgca ggacatccag gatgcgcagg gcaacccccc
     2641 ggagggtccg cggatgggaa atgatgaccc gcaggtcgac gtagaagggg gagagggcgc
     2701 cggacttgag gcggaactcg ccgaacttca gcgcctggat gccgaacagg tcgtcgacga
     2761 gccgcatctc cgaatcagtg agcatggttc tcctcgtccg gcactcgata gtctcgacaa
     2821 taatgccctg tcccgcagca ttttgcgggg tatcgtctcg tactttggta cgtcattccg
     2881 tgcttgacac ggaatccagt ggggtcatct ggataccggc tttcgctggt atgacgagct
     2941 tacggcaagc cacgggggag aaccctcatg gactcaatga ccaactcgaa gttctctcgt
     3001 agttcattgg gatttactta ttgttcattg gccctctggc attcggctgc aatctgttgc
     3061 gccgccgcgg ccg
//
LOCUS       DBNGZC010001021         2031 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2633452, whole genome shotgun sequence.
ACCESSION   DBNGZC010001021 DBNGZC010000000
VERSION     DBNGZC010001021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2633452"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="AC16G0_17590"
     CDS             <1..778
                     /locus_tag="AC16G0_17590"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HIR5101471.1"
                     /translation="GYLVVFGEILLENLGLPVPGIVVLIVGGAFAAAGRLSLLLVVVL
                     AILAAVLGDVVWYALGRWRGRPVLTFLCQLSLNPDTCVGTTERFFLRYGMPTLIVAKF
                     LPGVNTVTPPLLGILRTRLWPFLAYDLGGALFYTAVAVGLGYVVGIEVVHQAQAAASR
                     MGLWLGWGALAVGLVSLGCRVWVRFKVHRALRTVGLTPEELWRRRAGENNLVVIDVRT
                     QLAVQEQPERIPGAMRGGHEELDRMAGTLPRGGTIVTYCV"
     gene            1035..1709
                     /locus_tag="AC16G0_17595"
     CDS             1035..1709
                     /locus_tag="AC16G0_17595"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR5101472.1"
                     /translation="MPTSSSVTFSLDAGKASGYLARPGKGIPGVIVLQEWWGLVPHIK
                     DVAGRFAGQGYLALAPDLYHGKSTLEAAEAEHLMKGLDWARAAKEIAGAVRHLREVEG
                     ATKVGVVGFCMGGALTIIAATQPGVDAYAAFYGFPPAGAAPLEKVAAPGLLFFGEQEP
                     FFSVPDAKAFAEAQRKRGREAEVIIYPGAGHAFFNNERAEAYRKDAANDAWRRTLELF
                     GRHLRG"
BASE COUNT          298 a          640 c          710 g          383 t
ORIGIN      
        1 tggatacctc gtggtcttcg gggagatcct cttggaaaac ctcggcctgc cggttcccgg
       61 catcgttgtc ctgatcgtcg gcggcgcctt cgccgccgca ggccggctct ccctcttact
      121 cgtcgtcgtc ctcgccatcc tggcggcggt cctgggcgat gtggtctggt acgccctggg
      181 ccgctggcgg ggccggccgg tcctgacgtt cctctgccag ctctccctca accccgacac
      241 ctgcgtgggg acaacggaac gcttcttcct gcggtacggc atgccgaccc tcatcgtggc
      301 aaagtttctg cccggagtga acacggtgac gccgcctttg ctcgggattc tccggacgcg
      361 tctctggccg tttctggcct acgaccttgg gggtgccctg ttctacacgg ccgtggctgt
      421 gggactcggg tacgtggtcg ggatcgaagt cgtgcaccag gcccaggcgg ccgcttcgcg
      481 gatgggtttg tggctcggtt ggggcgccct ggccgtcggt ctggtctccc ttggctgtcg
      541 agtctgggtt cgattcaagg tccatcgagc cttgcggacc gtcggtctca cgccggagga
      601 gctttggcgg cggcgtgcgg gagagaacaa cctcgtcgtc atcgacgtgc gcacgcagct
      661 tgcggtccag gagcagccgg agcgcattcc cggcgccatg cggggaggac atgaggagct
      721 cgataggatg gcagggacac tgccgcgcgg tgggacgatc gtcacctact gcgtctgacc
      781 caacgaggtc tcgagcgtcc gggcggcgct caagcttcgg gcgcaggggt tcccggacgt
      841 atgggtgctc cgcggggggc tgaatgcctg gatccgggcc ggctatccca ccgaaccctt
      901 tcaggagatg gcggcgtgag gcctgaccag gcctcttgcc tttgtcttgg aggagaagca
      961 gacctcccgt gccacgagga tattcccggc ggcagtcggg aggcaagacc aaagcgttcg
     1021 aacagggggg gattatgccg acatccagca gcgtgacgtt ttccttggac gccgggaagg
     1081 cctcggggta tctcgcacgg ccgggcaaag ggattcccgg ggtcatcgtg cttcaggaat
     1141 ggtgggggct ggttccgcac atcaaggatg tggccggacg cttcgcaggg caggggtacc
     1201 tcgccctcgc ccccgatctg tatcacggga agagcacctt ggaggcggcc gaggctgagc
     1261 acctcatgaa gggtctggat tgggcccggg ctgccaaaga gatagccggc gcggtgcgcc
     1321 acctccgcga ggtcgagggc gcgacaaagg tgggtgtcgt gggcttctgc atgggcggcg
     1381 ccctcacgat catcgcagcc acccagccgg gggtcgatgc ctatgccgcc ttctacggct
     1441 tcccgccggc tggcgccgct cccctggaga aggtcgcggc gcccgggctc ctcttcttcg
     1501 gcgaacagga gccgtttttc tctgtgcccg acgccaaggc cttcgccgaa gcccagcgaa
     1561 agcgcggccg ggaggccgag gtgatcatct acccgggtgc agggcacgcc ttcttcaata
     1621 acgagcgagc cgaagcctac cggaaggacg ccgccaatga cgcctggcgg cggacgctgg
     1681 aactctttgg tcgccatctc cggggatagg gagatggtga gcgtctgatg ccggtggggg
     1741 acccctcacc tctccgaccg ttccggaccg cgtcctgcct ccgccgaccg cgcgtccggt
     1801 caatttcaac ttgacacaga cagcgggaat gatagcgtct ggctccctgg aacatctgcc
     1861 tgccgcaagg agaggtgcgg agtcatgaga catcggccgg attgcgcggt cggcggtgac
     1921 ggttcttggt tggagatcag cccggacgtg cggccctctg tggatggatg acccccagag
     1981 ggccgtgctg tattcagagt tccggcgggc tctcccagcg ccttgcggct g
//
LOCUS       DBNGZC010001022         3298 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1264410, whole genome shotgun sequence.
ACCESSION   DBNGZC010001022 DBNGZC010000000
VERSION     DBNGZC010001022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1264410"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="AC16G0_17600"
     CDS             complement(<1..990)
                     /locus_tag="AC16G0_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5101473.1"
                     /translation="MGTTRDIKQRKLVEETVISHPPPRGMHVPAVEVKDLRHMYGVRE
                     ALAGVSLTVFRGEIFALLGPNGGGKTTLFKILCTLMSATSGEVRLFGHDLGRESDAVR
                     RRLGVVFQRPSLDPKLTVLENLRHHGHLYGMRGEPLRARVQAMLDRLGLADRSRDRVE
                     TLSGGLQRRTELAKALIHQPELLLLDEPSTGLDPGARRDFTHYLGHLRDQEGITALLT
                     THILDEAERCDRVGILHHGKLVALGTPEELKAQVGGDVVVIHSHTPEALRTKMRERFG
                     CESALVDGNLRLELPRGHEFVREVVEEFPREVESVTFGKPTLEDVFIHLTGHRL"
     gene            complement(1034..1939)
                     /gene="cyoE"
                     /locus_tag="AC16G0_17605"
     CDS             complement(1034..1939)
                     /gene="cyoE"
                     /locus_tag="AC16G0_17605"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR5101474.1"
                     /translation="MTLHVAAAHPTQACARRRTADFLILTKPRVVLMVLVSTAVGFYL
                     GAPGPVDAFRLLHSLLGTALAAGGTIALNQFLERDGDTLMERTRHRPLPDRRMEPGQA
                     LCFGTVITAGGILYLALFVDALPALVTVLTTGSYLFLYTPLKRKTTLCSIPGAVSGAL
                     PPVAGWVAAGGEFGPGAWILFAILFLWQIPHSLAIAWIYRADYARAGIRLLPVIDPEG
                     ASAGWQVVNYSLALLTTGLFPTLTGLTGPLYFYGALMLGVGLLACGVVLAVSRSVEDA
                     RRLLYASLLYLPALLVLMALDKARF"
     gene            complement(1936..2895)
                     /locus_tag="AC16G0_17610"
     CDS             complement(1936..2895)
                     /locus_tag="AC16G0_17610"
                     /EC_number="1.17.99.9"
                     /inference="COORDINATES: protein motif:HMM:NF014665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIR5101475.1"
                     /translation="MAEPKRLRAHTEPAANPWPHRVAVVTAGATFVLILVGGLVTNTE
                     SGLAVPDWPTTFGYNMFLYPWSQMVGGILYEHSHRLIGSVVGLLTLTLTVVTWAVEPR
                     RWLRWLAGAALAAVILQGVLGGLRVVLVAETLAIIHGILAQAFFGLMGSLVLFTSREW
                     SEPVMPLRSGDAGALRWLSLLTTVTIFLQIVFGALLTHLEARLDAHLTLAALIAVLVP
                     LLGGRVWGNQAEHRKITRSVAILCGLLGLQLLLGLGSYVGRFAEFSLPLAPVSRLALP
                     IAHRLTGGLMLVTSLALTLRLHKLLGSDESVPDRGSVSRRVSA"
BASE COUNT          640 a         1100 c         1068 g          490 t
ORIGIN      
        1 gaggcggtga cccgtcaggt ggatgaaaac atcctccagg gtcggctttc cgaaggtgac
       61 ggactcgacc tcgcggggaa actcctccac aacctcccgg acgaactcgt gcccgcgagg
      121 cagttccagg cggaggttcc catccaccag ggccgattcg cagccgaacc gctcgcgcat
      181 cttcgtccgg agagcctccg gagtgtggga atggatcacg acgacgtcgc cccccacctg
      241 agccttcaac tcttccggcg tccccagagc caccagcttg ccatggtgga gaatccctac
      301 ccggtcacag cgctcggcct cgtccaggat gtgagtcgtg aggagcgcgg tgatcccctc
      361 ttggtctcgc aggtgaccga ggtaatgcgt gaaatcccgg cgggcgccgg ggtcgagacc
      421 agtgctgggc tcgtccagga gcaggagttc cggctggtgg atcagcgcct tggccagctc
      481 ggtccgccgc tgcaggcccc cggagagggt ctccacccgg tcgcgggagc gatcggcaag
      541 ccccaggcgg tccagcatgg cctgcaccct cgcccggagg ggctcgcccc gcatgccgta
      601 gaggtgcccg tggtgccgga ggttttccag gaccgtgagc tttggatcca gactgggacg
      661 ctgaaagacc acgcccagac gccgcctgac ggcatccgac tcgcggccca gatcatggcc
      721 gaacagccgc acctccccag aggtggcaga catgagggtg cagaggatct tgaagagggt
      781 ggtcttgcct cccccattcg gtccgagcag agcgaaaatc tccccccgga aaacggtcag
      841 gctgacgccg gccagcgcct cccggacccc atacatgtgg cgaaggtctt tcacctcgac
      901 cgccgggacg tgcatcccgc gtggtggggg gtgtgagatc accgtctcct cgaccagttt
      961 tctctgcttg atgtctcgcg tggtccccac gtatagcccg acctttcttg acaggacacc
     1021 gtcgtcagcc tggctagaac cgcgccttgt ccagagccat caggacgagg agggccggca
     1081 ggtacaacag ggaagcatac aacagccggc gcgcatcctc caccgaccga gagaccgcca
     1141 ggacgacccc acacgcgagg agacccaccc ccagcatgag cgccccgtag aagtagagcg
     1201 gcccggtgag cccggtgagc gtggggaaga gacccgtggt caggagtgcc agcgagtagt
     1261 tcacaacctg ccagccggcg ctcgccccct cagggtcgat cacgggcagc aaccggatgc
     1321 ccgcacgggc gtaatccgcg cgatagatcc aggcgatggc cagggaatgg gggatctgcc
     1381 agaggaacag gatggcgaag agaatccagg cgccgggccc gaactccccc ccagctgcga
     1441 cccacccggc caccggcggc aaggccccgg agaccgcgcc cggtatgctg cacagcgtgg
     1501 tcttgcgctt gaggggagtg tacaggaaca agtagctccc cgtcgtgagc accgtcacca
     1561 gggcggggag ggcgtccacg aagagggcca ggtacaagat cccgccagca gtgatgaccg
     1621 tcccgaagca gagcgcctga cccggctcca tccgcctgtc gggcagcggc cgatggcgcg
     1681 tgcgctccat tagggtgtct ccatcccgtt cgagaaattg atttagggcg atcgtgcctc
     1741 ccgccgccag cgccgtcccc agcaggctgt ggagcagccg gaaagcgtcc accggtccgg
     1801 gagcaccgag gtagaacccc accgcggtgc tgaccaggac catgaggacc acccgcggct
     1861 tcgtcaggat gaggaaatcc gccgtccgtc gccgcgcgca cgcctgcgtt gggtgtgccg
     1921 ccgccacatg cagggtcatg ccgacacccg cctcgagacc gaccctcggt cgggaacaga
     1981 ctcgtccgat ccgagcagct tgtgcaggcg cagggtcagg gcgagactgg tgacaagcat
     2041 caaccctccc gtgaggcggt gggcaatagg aagcgccaac cttgagacgg gagccagggg
     2101 caagctgaat tccgcgaacc gacccacata ggagccgagg cccagcagga gctgcagtcc
     2161 caggagcccg cacaggatgg ccaccgaccg ggtgattttc cgatgctccg cctgattacc
     2221 ccacacccga ccaccgagca ggggcaccag gacagcaatc aacgcggcca gggtgaggtg
     2281 agcgtcaagc ctcgcctcca gatgcgtgag caaggctcca aagacgatct ggaggaagat
     2341 ggtcacggtg gtgaggagac tcagccatcg cagggcaccg gcgtccccgc tccgcagtgg
     2401 catgacaggc tcgctccact cccgggaggt gaagaggacc aggctcccca tcagaccgaa
     2461 gaaggcctgg gccaggatgc cgtggatgat ggcaagggtt tccgccacca agaccacgcg
     2521 cagacccccc aggaccccct ggaggatcac cgcggcgagc gccgcgcccg ccagccagcg
     2581 aagccaccgt cgaggctcta ccgcccatgt caccacagtc agggtcaggg tcagaaggcc
     2641 gacgaccgag ccgatgagcc ggtggctatg ctcgtagagg atcccgccga ccatctgcga
     2701 ccagggatac aggaacatgt tgtacccgaa ggtggtcggc cagtccggca ccgccaggcc
     2761 tgactccgtg ttggtgacga gtccgccaac caggatcagg acgaaagtgg ctccggctgt
     2821 cacgacggcc actcgatggg gccaaggatt cgcagccggt tcggtgtgcg cccgaaggcg
     2881 ctttggctct gccattcgcc gtctccttcg cgtgggacgc cgactccccc gagacggggg
     2941 catcgggttt ggaggtgaat ctccacgggg tcactggtct gatttcgaag ggaaggatga
     3001 catgagaccg cctggagatg gcgccttcaa gatctcggcg gctcatcacc ggaaactctg
     3061 gcaggtgtct cccgtcagcc agaagtctgc cgtcactgtt ttataagccc ctaaggcaga
     3121 tcgttcaagc ccgaacctca tgcgtcgcgg tacgctacgg gcgaagaggg ccggaacttg
     3181 acctggagcg tatttaccgt gcggtgcgga agttaggcga cgcttccgcc acagccgcac
     3241 agtccgggat caccttctcc agttcatccc agctttcgtc cacccggcac acatccgt
//
LOCUS       DBNGZC010001023         3698 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1459487, whole genome shotgun sequence.
ACCESSION   DBNGZC010001023 DBNGZC010000000
VERSION     DBNGZC010001023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3698)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3698)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3698
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1459487"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="AC16G0_17615"
     CDS             <1..1245
                     /locus_tag="AC16G0_17615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR5101476.1"
                     /translation="IVSTPIVFSLALSAVTLLWNRDMLVPQLMVQRMFAGIDSFPMMA
                     IPFFMLAGSLMDTGGISTRLVRFSNYLVGWIQGGLAHVVIVASCFFAGITGSAVADAT
                     AIGSTLIPIMNKRGFPKLFSATVVAAAGVVGPIIPPSIPMVLYGVMAGVSIGALFMGG
                     FIPGIMIGLGLMAVAYVQAKKNNYPRETVRPTAREFFEATISAMGAIFMPLIILGGIF
                     LGVFTATEAAVVATVYAFIMGKFVYRELQWRDLPEILYRGGLSTAMILIIVGVANLVG
                     YIMAVERIPLQVAELFLSITSNKWMMLLLINILLLIVGCFIDGASALIIFTPVLLPLI
                     QKLGIDPIFFGVVICVNLMIGTITPPVGLCLYVACGIADVRLEQISRTIIPFLVVEIV
                     VLFLITYVPNLIMFLPHLFGMR"
     gene            1332..2162
                     /locus_tag="AC16G0_17620"
     CDS             1332..2162
                     /locus_tag="AC16G0_17620"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR5101477.1"
                     /translation="MDRIISVSSMAFDGYSLEQALDELAALGVRYVEPASVDKVFQHL
                     VETDFCEARAAWLQDQMGRRNLSCLSLSAHVDLTQADSTGRFLRRLEFAHAIGARIVN
                     TIAGPAGNLRGFQANIPAIAKRAADLGIVVALENHGDLVDRGRQIVEFVQAVGSPAIR
                     ANYDTGNAWYYARGGLNPAKELAEIAPVVAHVHLKAPRMDDGMMRWVALGEGVLDLPA
                     VGRVLREHLPGVPVSIELSLRQRSRDFEPRWRTPEVPSLPEIRGIITRSLLALEGVLS
                     "
     gene            2365..3507
                     /gene="thiO"
                     /locus_tag="AC16G0_17625"
     CDS             2365..3507
                     /gene="thiO"
                     /locus_tag="AC16G0_17625"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HIR5101478.1"
                     /translation="MPVQKQDVLIVGGGIIGCSIAYFLAKAGVKPLVMERDQVGAEAS
                     SGAAGLLTAQAHSDETGPLLDLKLASRALYATLALELRERTDMDIEYRTLGHLVPALT
                     EAELTVVNKRVAWQVSLGLPDIRLTAHEARELEPGLTPEVMGGGWFPEDHHVNNTAVT
                     QALASATIRLGGQVRTGTAVVEVLCTGERVTGVRTALETVAAGTVVLSAGAWSRDFEA
                     SAGLPLPIVPVKGQIVVARLEKPALRHVVYGEAGYAIPRASGEHILGSTVEYVGYDKR
                     VTLEGLTGIMAGITRMVPTLQQAEMAASWGCLRPAAPDGLPLLGRVPGREGLVVATGH
                     FRNGILLAPITGKLIAELIEEGKASIPLDPFRPDRPFPSGLPPQAY"
BASE COUNT          606 a         1165 c         1214 g          713 t
ORIGIN      
        1 attgtcagca ccccgatcgt ctttagcctg gccctgagcg ccgtgaccct gctctggaac
       61 cgagacatgc tggtcccgca gctcatggtt caacgcatgt tcgcgggcat cgactccttc
      121 cccatgatgg ccatcccctt cttcatgctg gcgggctccc tcatggatac cggaggaatc
      181 tccacgcggc tggtccgctt ctccaactac ctggtgggct ggatccaggg gggtctggcc
      241 cacgtggtga tcgtggcctc ctgcttcttt gccggcatca cgggctcggc cgtggcggac
      301 gccacggcca tcggctcgac cctgatcccc atcatgaaca agcgggggtt ccccaaattg
      361 ttctcggcca ccgtggtggc ggccgccggc gtcgtggggc cgatcatccc gcccagcatc
      421 cccatggtcc tgtacggggt catggcggga gtctccatcg gcgccctgtt catggggggc
      481 ttcattcccg ggatcatgat cggcctcggc ctcatggcgg tggcctacgt gcaggccaag
      541 aagaacaact acccgcggga gacggtgcgg cccaccgcca gggagttctt cgaggccacg
      601 atcagcgcca tgggcgccat cttcatgccc ttgatcatcc tgggcgggat cttcttggga
      661 gtgttcacgg ccaccgaggc cgccgtcgtg gcaacggtgt acgccttcat catgggaaag
      721 ttcgtctacc gggaattgca gtggcgagac cttcccgaga tcctgtacag ggggggcctg
      781 agcacggcca tgatcctcat catcgtcggg gtggccaatc tggtcggcta catcatggcg
      841 gtggagcgga ttcccctgca ggtggccgag ctgtttctta gcatcacctc gaacaagtgg
      901 atgatgctgc tgctcatcaa catcctgctc ctcatcgtcg gctgcttcat tgacggcgcc
      961 tcggccctga ttatcttcac gcccgtgctc ctgcccctga tccagaagtt ggggatcgac
     1021 cccattttct ttggggtggt gatctgcgtg aatctgatga tcggcaccat cacgcccccc
     1081 gtgggcctct gtctctacgt ggcctgcggg atcgccgacg tccggttgga gcagatcagc
     1141 cgcacgatca tcccgttcct ggtcgtcgag atagtggtgc tgttcctcat cacgtacgtg
     1201 ccgaatctga tcatgttcct cccgcacctg ttcggcatgc ggtagaaagt cagaaggcgt
     1261 tcgaccgttc gacggttcag ccgtcgggga cgagagaatc ctcgaacagc cgaaggcctt
     1321 gcgagggaat cgtggatcgg atcatctccg tgagcagcat ggccttcgac ggctactcgc
     1381 tggagcaggc gctggatgag ctggcggcgc tgggcgtccg atatgtggag ccggccagcg
     1441 tggacaaggt tttccagcac ctggtggaga cggatttctg tgaggcccgg gccgcctggc
     1501 tgcaggacca gatggggcgt cgcaaccttt cctgcctgtc tctgtccgcc catgtggacc
     1561 tcacccaggc ggattcgacg ggccgattcc tgcgtcgact agagtttgcc cacgccatcg
     1621 gcgcccggat cgtgaacacc atcgccgggc cggccgggaa tttgagaggg tttcaggcaa
     1681 acatcccggc gatcgcgaag cgggcggcgg acctgggaat cgtcgtggcc ctggagaacc
     1741 acggggacct cgtggatcgg gggcggcaga tcgtggagtt cgtccaggcg gtgggcagcc
     1801 cggcgatcag ggccaactac gacaccggga acgcctggta ttatgcccgg ggaggcctga
     1861 atcccgccaa ggagctggcc gaaatagcgc cggtcgtcgc ccatgtccac ttgaaggcac
     1921 cgcgcatgga cgacggaatg atgcgctggg tggccctggg ggagggggtc ctggaccttc
     1981 cggccgtggg ccgcgtcctc cgcgagcacc tgcccggagt tcccgtcagc atcgagctgt
     2041 cgctccgtca acggtcgcgg gacttcgagc cccgctggcg aacccccgag gtcccttccc
     2101 tgccagaaat ccgcggcatc attacccgct cgctcctggc tctcgaaggc gtcctatcct
     2161 gatgtcccta tccgggggaa gtcgaggtcc ggatcaagga ttccgcaggc atgccgtctg
     2221 gacctgcgcg cttcccatgc tacaatgcgc ctgagtcgcc ggatgatccg atcgacggga
     2281 cctaacatta caggggaaag tcggggtgcc gggggctagc tttcccgagt cccgtctggc
     2341 cgtgcagaat gacccgagga gaggatgccc gttcagaagc aggatgtcct catcgtgggc
     2401 ggggggatca tcgggtgttc catcgcctac ttcctggcga aggccggggt gaagccgctc
     2461 gtcatggaac gggaccaggt gggagcggaa gcttcgagtg gggcggcagg cctactgaca
     2521 gcccaggcgc attccgacga gacgggtccg ctgctggacc tcaagctcgc cagccgggcg
     2581 ttgtatgcca cgctggcact cgagctccgc gagcggacgg acatggacat cgagtaccgg
     2641 acgctgggtc accttgttcc cgcgctcact gaagcggaac tcaccgtcgt caacaagcgg
     2701 gtcgcctggc aggtttccct gggactgcct gacatacggc tcaccgctca tgaggcgcgc
     2761 gagctggagc ccggcctgac cccagaggtg atgggaggag gctggttccc ggaggatcat
     2821 cacgtcaaca acacggctgt cacccaggcc ctggccagcg ccacgatacg cctgggcggt
     2881 caggtgcgca cgggcactgc cgtcgtcgag gttctgtgca caggcgagcg agtgacgggg
     2941 gtgcggacgg cgctggagac cgtggctgcc ggcaccgtcg tcctttctgc cggggcctgg
     3001 tcgcgggatt tcgaggcgtc ggcgggactt cctcttccca tcgttccagt caaggggcag
     3061 atcgtggtgg ccagactgga gaaacccgcg ctccgtcacg tcgtgtacgg cgaagcaggc
     3121 tatgcgatcc cgcgtgcttc gggcgagcac attcttggca gcacggtgga gtacgtcggc
     3181 tacgacaagc gcgtgaccct cgagggattg acgggcatca tggcgggaat cacccgcatg
     3241 gtcccgaccc tccagcaggc cgagatggct gcgagttggg ggtgcctgcg gcctgcggcc
     3301 ccggatggcc taccccttct ggggcgagtc ccggggcgtg agggtttggt tgtggccacc
     3361 ggtcacttcc gaaatgggat cctgctggcc cctatcacgg gaaagctcat cgccgagctg
     3421 atcgaagagg gaaaggcttc gatccccctg gaccctttcc gccccgacag accgtttccg
     3481 tccggcctgc ctccgcaagc ctactgacct cgattctccg aaccgccaag actgaacgtt
     3541 ctccttagga tcgaggatgt caccagactc tgtctggacg gcaatgccgc cacgcctagg
     3601 tcgttccagg tgtttgatcg gaggggtctc agaggcgacg gacaggcact ttcgaccggc
     3661 ttccagaatt tccctgggag gaaggaccgt tgtcggag
//
LOCUS       DBNGZC010001024         3903 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2436748, whole genome shotgun sequence.
ACCESSION   DBNGZC010001024 DBNGZC010000000
VERSION     DBNGZC010001024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3903)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3903)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3903
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2436748"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..588
                     /gene="aroF"
                     /locus_tag="AC16G0_17630"
     CDS             <1..588
                     /gene="aroF"
                     /locus_tag="AC16G0_17630"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR5101479.1"
                     /translation="GEEGLRLLAAARDETGLAIVTEVLKPEDVDLVAETADMLQIGAR
                     NMQNFALLKRVGEARKPVLLKRGISSTIQEFLMAAEYVLSEGNYDVVLCERGIRTFET
                     ATRFTLDLNAVPVIKKLSHLPVMVDPSHGTGHWEYVTAMAKAGIACGADALMIEVHVR
                     PEIAFSDGIQSLKPSLFRQLMQEIAPFVQAAGRRL"
     gene            complement(611..1324)
                     /locus_tag="AC16G0_17635"
     CDS             complement(611..1324)
                     /locus_tag="AC16G0_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020265377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5101480.1"
                     /translation="MRLEVADLHVAYQRDLFVLRGLDLVAAPGKITIVIGPNGSGKST
                     LLKTIAGNLKAGKGRVMLDGQDITGIESDEGISRGISYIPQDRAVFPQMTVTENLELG
                     CWIFRKDRARVRERLAWALERFPALMEKRRLRAGELSGGLQRMLDIAKSLLSNPSLLL
                     VDEPSVGLAPLVAQTIYAELHSLRQEGRTILLVDQDVRSAMQIADYVYVLDLGTVQTH
                     GSRDAFEGKLGEMVRSWLL"
     gene            complement(1321..2037)
                     /locus_tag="AC16G0_17640"
     CDS             complement(1321..2037)
                     /locus_tag="AC16G0_17640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5101481.1"
                     /translation="MTLWIEAVSKHFGGLQALCDVSISFAAGEFAGLIGPNGSGKTTL
                     INCISGLCRPDAGSIRFGRHTISRLPPHRIYRLGIGRTFQISKVFNRLTVLENLRIPA
                     LTEGKLPRREVDGRSREILKQINLAQHGHVNAENLSGGQRKLLELGMIMMTDPQFLLL
                     DEPFGGVHPELRNQLEGYLRWLNQAGKTIILVSHEMASVFRICQRLVVLDHGVLITDG
                     PPEAVRSDERVISAYLGGQA"
     gene            complement(2034..2972)
                     /locus_tag="AC16G0_17645"
     CDS             complement(2034..2972)
                     /locus_tag="AC16G0_17645"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5101482.1"
                     /translation="MKGIVPGLLFVAAVAFPLFFQDYWLYVATIGLYYAILASSWSML
                     AGQVGLISFAHAAFAGIGAYTSGLLVTHLGLPIPVAMVGGALAAAPIGLGIGALTLRM
                     RGPYLALTTLAFSEIFRIFITAEYGLTQGSLGLKVPLMLGGSKPLSYYTGLGLFLVTV
                     ATLNGVLRSRVGLFLVAMREDEDGAETRGVNTVLYRLIAFVVTSAFAGLAGGFYAHVV
                     GLVSPQMTRIGEMGLILAMSVFGGLESLLGAALGAIILQGLTEYLRIFAEWRLAIFGA
                     LVLFTLKFAPAGILPSLYASGDRLVHRWWSRGPQSP"
     gene            complement(2969..3859)
                     /locus_tag="AC16G0_17650"
     CDS             complement(2969..3859)
                     /locus_tag="AC16G0_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007068600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR5101483.1"
                     /translation="MLFLSQFANALVIGTLYALMALGLSFVFGVLKLVNFAHGEAIMV
                     GGYLYYLLASLKLPLPLALLSSIPALFLFGVFLERLFLRAPYQHKMARYDEYVILVTF
                     ALGIFLQNLAFPIFGPYNRQAPPLFVGTIRLGELVVAGDRLAGALIAFATIGVLIFLL
                     YRTYLGKGLRAVAQNPGAASIVGIKVLRMRSVGFGIGVTLAGVAGALFGPIFLVNPEM
                     GTSFAVKAFVIVVLGGMGSVTGSILGAYILAIVESFGSILLPDPTRALAYKDAYGLLL
                     LVVVLLARPQGLFGERERRA"
BASE COUNT          787 a         1254 c         1264 g          598 t
ORIGIN      
        1 ggagaggaag gcctccggct cctggcggcg gcacgagacg aaacaggatt ggccatcgtg
       61 accgaggtct tgaagccaga ggacgtggac cttgtggccg agaccgcgga catgcttcag
      121 atcggggccc gcaacatgca gaacttcgcc ctgctgaagc gagtgggcga ggcgaggaag
      181 ccggttctcc tcaagagagg catctcgtcg accatccagg aattcctgat ggcggcggag
      241 tacgtcctct cggagggcaa ctacgatgtg gtcctctgcg agcgaggcat ccggaccttc
      301 gagaccgcca cccgcttcac cttggacctg aatgcggtcc cggtcatcaa gaagctgagc
      361 cacctgccgg tcatggtgga ccccagccac ggcaccggcc actgggagta cgtcacggcc
      421 atggccaagg ccggcatcgc ctgcggggcg gacgccctca tgatcgaggt ccacgtccgt
      481 cccgagatcg ccttcagcga cggcatccag tccctcaagc cgtccctgtt ccgacaactg
      541 atgcaggaaa tcgccccctt cgtccaggcg gccggccggc gcttataggg ccaggaagcg
      601 ttcacgttcc tcagagaagc cagctccgca ccatctctcc aagctttccc tcgaaggcgt
      661 cccgggatcc gtgcgtttgc accgtcccga gatccaggac gtacacgtaa tcggcgatct
      721 gcatggccga gcgcacgtcc tggtcgacga ggaggatggt ccgcccctcc tgtctcagtg
      781 aatgcagctc cgcgtagatg gtctgggcca ccaggggcgc cagccccacg ctgggctcgt
      841 ccacgagaag aagcgatgga ttggagagca gcgacttggc gatatccagc atgcgctgca
      901 gtcctccgga aagttctccg gcgcgcagac ggcgcttttc catgagcgcc ggaaaccgct
      961 ccagcgccca ggccagtcgc tcgcgcaccc tcgcccggtc tttccggaag atccagcagc
     1021 caagctccag gttctcggtc acggtcatct gcgggaacac cgcccggtcc tgagggatat
     1081 aggagatacc ccgcgagatg ccctcatccg actcgattcc ggtgatgtcc tgcccatcca
     1141 gcatcacgcg acccttgccc gccttcaggt tgccggcgat ggtcttgagc agggttgatt
     1201 tgccggagcc gttgggcccg atgacgatgg tgatcttgcc cggagcggcg acgaggtcca
     1261 gccccctcag aacgaagagg tctcgctggt aggccacgtg caggtccgcc acctcgagcc
     1321 tcatgcttgc ccccccagat aggcactgat cacccgttcg tcggagcgga ccgcctcggg
     1381 agggccgtcg gtgataagga ccccgtggtc cagcaccacc aggcgttgac agatcctgaa
     1441 gacggaggcc atctcgtggc tgaccaggat gatggtcttg ccggcctggt tcagccagcg
     1501 caggtacccc tccaactggt tcctcagctc cggatgcacg ccgccgaatg gctcgtccag
     1561 caacaggaac tgcggatccg tcatcatgat cattccgagc tccaggagtt ttcgctgacc
     1621 cccggagagg ttctcggcgt tcacgtgacc gtgctgcgcg aggttgatct gcttcaggat
     1681 ctcccgggac cgcccatcca cctcccggcg agggagcttg ccctctgtga gggcggggat
     1741 cctcaggttc tcgaggacgg tcagccggtt gaacaccttg gaaatttgaa atgtccgccc
     1801 gatccccagc cggtagattc ggtgaggggg gaggcgggag atcgtgtgcc ggccgaaccg
     1861 gatcgagcct gcatccggcc ggcacagccc cgagatgcag ttgattaggg tggtcttgcc
     1921 ggacccgttg ggaccgatca gacccgcgaa ctcccctgcg gcgaaagaga tcgacacgtc
     1981 gcagagggcc tggaggccac cgaagtgctt gctgaccgcc tcgatccaga gcgtcatggc
     2041 gactgcggcc ctcgactcca ccaccgatgc accagcctgt ccccagacgc gtacaggctg
     2101 ggcaggatcc ccgcgggcgc gaacttgagg gtgaagagaa ccaaggcgcc aaagatggca
     2161 aggcgccact cggcgaagat ccggagatac tccgtcaggc cctgcaggat gatggcaccc
     2221 agcgccgcgc ccaggaggga ttccaggcct ccgaagacgc tcatggcgag gatcaggccc
     2281 atctcgccga tccgggtcat ctgcggactc accagtccca ccacgtgggc gtagaagcct
     2341 ccggcgaggc ctgcaaaggc actggtcacc acgaaggcga tcagccggta gaggaccgtg
     2401 ttgacccccc gagtttcggc gccatcctcg tcctcgcgca tggccaccag gaacagaccc
     2461 acccgggatc gaaggacacc gttgagagtc gcgacggtca cgaggaagag acccagtccg
     2521 gtgtagtacg agagcggctt gctgcccccg agcatgaggg ggaccttcaa cccgagggat
     2581 ccttgggtca ggccgtattc ggcggttatg aagatgcgga agatctccga gaaggccaac
     2641 gtggtcaggg cgaggtaggg tccacgcatc cgcaaggtca gggcgccaat ccccaggccg
     2701 atgggcgcgg ccgccagggc gccgcctacc atcgccacag ggatcggcag gcctaggtgg
     2761 gtgaccagga gtcccgaggt gtaggcgccg atacccgcga atgccgcgtg ggcgaacgag
     2821 atgagtccaa cctggccggc cagcatcgac caggacgagg ccaggatcgc atagtagagg
     2881 ccgatggtgg cgacgtagag ccagtagtct tggaagaaga ggggaaaggc gaccgcggca
     2941 acgaagagca ggcccggcac gatgcccttc atgcgcgcct ctcccgctcg ccgaaaagcc
     3001 cctgcggccg ggccagcagc accaccacga gcaggagaag gccgtacgca tccttgtagg
     3061 ccaaggcgcg ggtggggtcg ggcaagagga tggagccgaa gctctcgacg atggcgagga
     3121 tgtaggcccc caggatgctg cccgtgacag agcccatgcc tcccagcacc acgatgacga
     3181 aggccttgac cgcaaagctg gttcccatct cggggttaac caggaatatc ggcccgaaga
     3241 gcgcccccgc gactcctgcc agggtgacgc cgatcccgaa acctaccgat cgcatgcgga
     3301 ggaccttgat ccccacgatg gatgcggccc ctggattctg ggcgacggcg cgcagacctt
     3361 ttcccaggta cgtccggtag agcaggaaga tcaggacgcc aatcgtggca aacgcaatca
     3421 gcgcgccggc cagccgatcc ccggcgacga ccagctcgcc caggcgaatg gttcccacga
     3481 aaagcggggg ggcctgacgg ttatagggac cgaagatggg gaaggcgagg ttctggagaa
     3541 agattcccag ggcaaaggtg accaggatga cgtactcatc atatcgcgcc atcttgtgct
     3601 ggtagggcgc ccgcaggaag agtctctcca ggaagacccc gaagagaaag agcgccggga
     3661 tgctggacag gagcgccagc gggagcggca gcttcagact ggcgagcaga tagtagaggt
     3721 acccccccac catgatggcc tcgccgtggg caaagttcac cagcttcagg acgccgaaga
     3781 cgaaagacag ccccagcgcc atcagggcgt acagcgtccc gatcaccagc gcattggcga
     3841 actgcgagag gaagagcatg caaggctctc gcgatcaccc cctccaaacc ggagggggtg
     3901 atc
//
LOCUS       DBNGZC010001025         2150 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1750137, whole genome shotgun sequence.
ACCESSION   DBNGZC010001025 DBNGZC010000000
VERSION     DBNGZC010001025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1750137"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="AC16G0_17655"
     CDS             complement(<1..773)
                     /locus_tag="AC16G0_17655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101484.1"
                     /translation="MSRRCENRRAAREARERGDIAPPQTLEAPLYRPFALLALAVTLG
                     VATPIGAVALYRLYAPAGHVPAIWPSLHAHLQIFGFAGVLIMGVGQHLLLRFSHGPIR
                     RPRCMSWVLGLIALGLLARLAAALSPGASLWVTSGLAEFLAYALFAAWVTACVRATDP
                     RFASDWLMVTGAWWFVAATGAEFAAIVRAVAIGIDPATAVPGPGSYVMSLYGGVFGWV
                     LGVALRVAPMFLAVRNLTRMGGAILLGLNVAVILGLLAEG"
     gene            complement(828..1817)
                     /locus_tag="AC16G0_17660"
     CDS             complement(828..1817)
                     /locus_tag="AC16G0_17660"
                     /inference="COORDINATES: protein motif:HMM:NF019352.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR5101485.1"
                     /translation="MSTRTFAASMGLIVLLGIARPGTAAGEEQVRRENAFSATTFSTA
                     PQTAEIKRFTIAVEEVLLKIEDGISYYAWTFGGTVPGPTLRVREGDHVEITLENHGTV
                     SHGIDLHAAAVAPGKAFAPVAPGGRHTLRFVARQPGLFMYHCSALPIITHIATGMYGV
                     LLVDPKEGRAPAKEFVLVQGEFYGEPDGSGVVRGDSTKMMEGSPTFVVFNGGIERYLT
                     EPLMVKPDELVRLYMVNAGPNTVSALHVIGTLFDRVSPDGNPRNALVGVQTQFIPPGG
                     GAVFEFHLHEPGDYPVVTHVMSHVYKGAIGVFRAEEPASDGSHGHPAPSAMRH"
BASE COUNT          408 a          684 c          706 g          352 t
ORIGIN      
        1 ccctcggcca gcaagccgag gatcacggcg acattcaatc cgaggaggat cgctcctccc
       61 attcgggtga ggttgcggac ggcgaggaac atgggggcca cgcgcagagc gacgccaagc
      121 acccacccga acacgccccc gtacaggctc atgacgtacg atcctggccc cggaacggcc
      181 gtggccgggt cgatcccgat ggcgaccgcc cggacgatgg cggcaaactc ggccccggtc
      241 gcggcgacga accaccacgc gccggtgacc atcagccagt cggaggcgaa acgcgggtct
      301 gtggctcgca cgcacgcggt gacccaggca gcgaaaagcg cgtaggcgag aaactcggcc
      361 aaacccgagg tgacccagag ggaggcgccg ggggacaggg cggcggccag gcgcgccagc
      421 aaacccaggg cgatgagccc caggacccac gacatgcatc gtggacggcg gataggccca
      481 tgggagaagc gtaacagcag atgctgaccc acgcccatga tgaggacacc ggcaaatccg
      541 aaaatctgca ggtgggcatg aagtgacggc caaatggccg gcacgtggcc ggcgggagcg
      601 tacaggcgat acagcgctac ggctccaatc ggcgtggcaa cgcccaaggt aacggccagg
      661 gcaaggagcg cgaagggacg gtagagcggg gcctcaaggg tctgaggagg cgcgatgtcg
      721 ccacgctccc gggcctcacg ggcggcccgg cggttttcgc agcgtctgct cacagcgcgc
      781 tctcccgaag cgacactcat ggccgttccc tttgccgaat gccgttgtca gtggcgcatg
      841 gccgaaggcg ccgggtggcc gtgggatccg tcggaggcag gctcctcggc ccggaagacg
      901 ccgatggccc ccttgtagac atgggacatc acgtgggtca ccaccggata atcccccggc
      961 tcatggagat ggaactcgaa gaccgcaccg ccgcccggtg ggatgaattg ggtctgcacg
     1021 ccgaccaggg cattgcgggg gttgccgtcc ggtgaaacac ggtcgaagag ggtccctatg
     1081 acgtgaaggg ccgagacggt gttggggccg gcattgacca tgtacaggcg caccaactcg
     1141 tcgggcttca ccatcagagg ctcggtcagg tagcgctcga tgccaccgtt gaagaccacg
     1201 aaagtgggag acccctccat catcttggtc gagtccccgc gcactacgcc gctgccgtcc
     1261 ggttccccgt agaattcccc ttgtaccaga acgaactcct tggccggcgc cctgccttct
     1321 ttcgggtcca ccaggagcac gccgtacatg ccggtggcga tgtgcgtgat gatgggcaac
     1381 gcgctgcaat gatacatgaa caaaccgggc tgacgggcga cgaatcggag cgtgtgccgt
     1441 cccccgggcg cgacgggtgc gaaggctttg ccgggcgcca cggctgcggc gtggaggtca
     1501 atcccgtggg agaccgtccc atggttctcc agggtgatct ccacgtgatc tccttctcgg
     1561 acccgtaggg taggtccagg caccgttccg ccaaaggtcc aggcgtagta gctgatcccg
     1621 tcctcgatct tcagcaggac ctcctcgacc gcgatcgtga agcgcttgat ctcggccgtc
     1681 tgcggcgccg tcgagaaggt ggtggcgctg aacgcgttct cgcgccggac ctgctcctcc
     1741 ccggcggctg tccccggccg ggcgattccc agaaggacga tcaggcccat gctggctgcg
     1801 aatgtccgtg ttgacatgcg tggctcctct atcagttgtc agacttcgcc ggcgagtgta
     1861 agagaaagat cccgcagggt ctgtgacttt agtcccacgc agtcgaacta tcaggcccgc
     1921 ggggtttcct ccacgctgcc ccacacgtta ccttcatggg accatttgtc tgaggagcct
     1981 tgacaatgtg catggggcgc ccatatgatt cgtgcccgga tatcgctgac cttttcttct
     2041 ccgtccctcc aaacagttgt tgtgcgaggt gcaatatgaa accgctggat acccctctgc
     2101 tacttgagat ctatcgtaaa atggtcgccg tccggacctt cgaggagacg
//
LOCUS       DBNGZC010001026         2841 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_772886, whole genome shotgun sequence.
ACCESSION   DBNGZC010001026 DBNGZC010000000
VERSION     DBNGZC010001026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_772886"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..188)
                     /locus_tag="AC16G0_17665"
     CDS             complement(30..188)
                     /locus_tag="AC16G0_17665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101486.1"
                     /translation="MPRILETLLRRCIALGHPRWSLDQITAALREAAAGRGPYAVLFA
                     DMLTTLGG"
     gene            complement(552..>2841)
                     /locus_tag="AC16G0_17670"
     CDS             complement(552..>2841)
                     /locus_tag="AC16G0_17670"
                     /inference="COORDINATES: protein motif:HMM:NF013986.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HIR5101487.1"
                     /translation="LAAEALRQPRRGLPSPASGNLGSKPSSGNGDGPTGRPLSLENVL
                     LLRDLVRPCQPRKPGNGQDAYLGIDIGSVSTNLVVLSDVGEVLHDIYVRTEGRPIEVV
                     SAGLRSIAEAFGDCIRIQGAGTTGSGRELIGALVGADTITDEITAHKTGAMYVSETLL
                     GEQVDTIFEIGGQDAKFIALHDGVVIDFAMNEACAAGTGSFLEEQAERLGVSIKEEFA
                     RLALQSRHPVRLGERCTVFMEQDVNAWQQRGATQEDLVAGLAYSVALNYLHRVVRGRP
                     IGEVIYFQGGTAYNDAVAAAFAGILGKRIVVPPYNGVIGAIGMALLAREKIRETGLPT
                     TFRGLDLGTVHYTLREFTCKACSNVCDMQEFTVEGAKTYWGDKCSDKFRQRRQTQRKP
                     VIPDLMTWRRERLLDGYAGPRGSGPRVGLPRAMFFYDRFPFWRTVLEDLGCAVVLSDE
                     TNRKIAQNGIDLSVAEPCYPIRVAHGHVQDLLDKGVDWILLPATINAEPTPDAAESQM
                     CPWAQTLPCVLRSVSRLKEIRERILSPSVHFRLGEGHAARDLLPLGHALGAGSHRTLR
                     AVAHGFAAQRAFRQALEAAGRQALAVLRRNREPGIVLVGRPYNIHDRAVNLDVPGKLR
                     DYYGINVLPMDCLPLDDEDILDVNENMYWHSGRRILAAGKLVARSQHLHIIFLTNFKC
                     GPDSYIKHFLGTACGKPFLSLQFDGHSNDAGVMTRCEAYLDSQGMLRWWRTPASVVNR
                     KSVDKFPARGAPLGPWNMLTNL"
BASE COUNT          517 a          879 c          914 g          531 t
ORIGIN      
        1 cgacggtaac gccggaaccc ttcatggtct taccctccca aggttgtgag catgtcggcg
       61 aaaaggacgg catacggccc tcgacctgcc gccgcctcgc gcagtgcggc ggtgatctgg
      121 tccagagacc agcgaggatg acccagcgcg atgcagcggc ggaggagggt ttcgagtatg
      181 cggggcatgg ctcacctcgt cttgcagctc gaccacgctg accatgccct atcaagaggg
      241 caattcgagt gccaaggaaa gaacgcaagt tggctgtgga agtggcgggg ccgatgtgct
      301 gattttcaaa tggcaaggag catcgtgcga tggatgtgtt gatgccgaga atccacgaga
      361 gcgcccaagc atgcagacgg cgaatctgaa gggctgccca aggaccttgg cgcttctgga
      421 tggggggagg acagcaggaa ggccgcggag tcgatgcgcc gacgggggag ggtggtcgcc
      481 agaggctcgg caaggcagcc cgtcatgcag gtcagatggc gcctgcgttt cacggacgat
      541 ggtgtgctga ctcagaggtt cgtcagcata ttccaaggcc cgaggggtgc tcctcgggcc
      601 gggaacttgt ctacggactt gcgatttacc acactagctg gtgtccgcca ccatcgaagc
      661 atgccctggc tgtccaggta ggcctcgcaa cgggtcatga ccccggcgtc gttgctatgg
      721 ccgtcgaact ggaggctcag gaagggcttt ccgcacgccg tccccaggaa atgcttgatg
      781 taggagtcgg gaccgcactt gaagttcgtc agaaatatga tgtgcaagtg ttgggagcgg
      841 gccaccaact tgcctgccgc caggatgcgc cgacccgagt gccaatacat gttctcgttg
      901 acatccagga tgtcttcatc gtccaggggc aagcaatcca ttggtaggac gttgatccca
      961 taatagtctc gaagttttcc cgggacgtcc agattgaccg cccggtcatg gatgttgtag
     1021 gggcgcccca ccaagacgat ccccggctcc cgattccttc ggagtacggc caacgcctgg
     1081 cgccccgccg cctccaaagc ctgccggaag gcccgctgtg cggcgaaccc gtgtgccacc
     1141 gcgcgaagcg ttcgatggga tcctgcaccc agcgcgtgcc ccagggggag caggtcgcgt
     1201 gcggcgtgtc cttctccgag acggaagtgc acgctggggg aaaggatccg ctcgcggatt
     1261 tccttgagtc gtgagacact ccgcaatacg cagggaagcg tctgggccca tgggcacatc
     1321 tgcgattccg cggcgtctgg ggtcggttcc gcattgattg tcgcaggcag gaggatccag
     1381 tccacgccct tatccaggag gtcctggacg tggccatggg ccacccggat cggatagcag
     1441 ggctctgcca cgctgaggtc aataccgttc tgggcaatct tccggtttgt ctcgtcggag
     1501 aggaccacgg cgcagccgag gtcctccaag acggtcctcc agaaggggaa ccggtcgtag
     1561 aagaacatcg cccgagggag tccaacccgg ggaccggatc cacgcggccc ggcatagcca
     1621 tccagcagcc gttcacgtcg ccacgtcatc aggtcgggaa tcaccggttt ccgctgggtc
     1681 tgtctccgtt ggcggaactt gtcactgcac ttgtcgcccc agtaggtctt cgcaccttcg
     1741 acggtgaact cctgcatgtc gcagacattc gagcaggcct tacaggtgaa ttccctgagg
     1801 gtgtagtgca cggtgccaag atccaggccg cggaaagtgg tggggagtcc tgtctctcgg
     1861 attttctccc gggccaggag ggccatcccg atggcgccga tgactccgtt gtagggcggc
     1921 acgacgatac gcttgcccag gataccggcg aaggcggccg caacggcgtc gttgtatgcc
     1981 gtgccaccct ggaagtagat cacctccccg ataggccgcc cgcggacgac ccggtgcagg
     2041 tagttgaggg ccacagagta ggcaagtccc gccaccagat cctcttgcgt cgccccccgc
     2101 tgctgccagg cgttgacgtc ctgctccatg aacaccgtgc accgctcgcc gagacgcaca
     2161 ggatgacgag actgtaaggc gaggcgggcg aattcctctt tgatggagac ccccaggcgc
     2221 tctgcctgtt cttccaggaa ggagcctgtc cctgctgcgc acgcctcgtt catggcaaaa
     2281 tctatcacca cgccgtcatg gagggcgatg aacttggcat cctgtcctcc gatctcgaag
     2341 atggtgtcca cctgctcccc gagaagggtc tcggacacat acatggcgcc ggtcttgtgg
     2401 gcggtgatct cgtcggtgat ggtgtcggcc ccgacgaggg ctccgatcag ctctcgaccg
     2461 gaacccgtcg ttccagcacc ttgaatccgg atgcagtccc cgaacgcctc ggcgatgctc
     2521 cgaaggcctg cgctcaccac ttcaatcggg cgtccctcgg tgcggacgta gatgtcgtgc
     2581 agcacctcgc ccacgtcgct gaggacaacc aggtttgtgc tgaccgagcc gatatcgata
     2641 cccaggtagg cgtcctgccc attcccgggc ttgcggggtt ggcagggtcg caccagatcg
     2701 cggaggagca gcacattctc aagactcagg ggtcggcccg tcggcccgtc tccgttccca
     2761 ctcgatggtt tgctgcccag gtttccgctt gccgggctgg ggagcccccg ccgcggctga
     2821 cgcagcgcct cggctgctaa c
//
LOCUS       DBNGZC010001027         6106 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1063292, whole genome shotgun sequence.
ACCESSION   DBNGZC010001027 DBNGZC010000000
VERSION     DBNGZC010001027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1063292"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1614
                     /locus_tag="AC16G0_17675"
     CDS             <1..1614
                     /locus_tag="AC16G0_17675"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR5101488.1"
                     /translation="LRGYVRDQGGGLVMLGGEESFGVGGYYRTPIEEALPVTMEARQK
                     VEVPSLAVILVIDRSGSMEMSTQDSRFTKMDLAKEAAQLVVELLDERNEVGVVAFDTA
                     WDWVVPIGPAKNKDRILKAISTIKAGGGTDLFPPLKDAYQTIFDRRALLRHVLVLSDG
                     EVQAADFAGLVRRMQMDKITVSSVAIGSDADVRFMTDLSRWGRGRFYYTDDIYSIPRI
                     FTLETQLASKASIIEEPFRVGVASEGHEILQDIRWDQAPPLGGYVSATPKPTADTLLL
                     SHQRDPILATWRYGLGRTAAFTSDAKGKWGILWLKWGDYAKFFGQMVRWTLRTTQQRE
                     VVTSVVQRDGRGEIQLEAVDQKGDFVNFLEANAGVISPDKVQRVLPLQQIGPGRYRGT
                     FDAANEGAYLVGVAERKDQKMVGSEVASLVIPYSPELRALSVNEPLLRDVAALSGGAA
                     PSQPGDVFTQHRRPARVWVEAWPQLLALALFLFLLDVAIRRFWLRGWALRRSAGRDGA
                     TRSGEGTGSWGGGSASPMAARFGSRGRRT"
     gene            1791..3134
                     /locus_tag="AC16G0_17680"
     CDS             1791..3134
                     /locus_tag="AC16G0_17680"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5101489.1"
                     /translation="MGAVLLLGAPSGWAGEQTATASSKVRLAVIPFTGPAAGVPEGFG
                     EALGQAIRHGLSQVRAVRPVDSGEILGSSQRLHISLADRLSDEAALRLVKDLQAQGLV
                     TGTYALDGESLRVQARLADPSGAGQVILGEEAIGPLAQFLSAQGRITRQILQAFRVRL
                     TPYDERRLQAAFSEQTGSLEAYALYGRAGWQQGLGTKEGYEQAVALLSKALETDQNFA
                     LAHFALGISLQATNNRWKASGEFRKTIQLDGTFADAHKWLGDLLVKSPRRLYDQAIQA
                     FQKALELSPDYAEAMVGLGDARQAKGQFDEAIGDYKRALQLEPENARVHFGLGKIYYN
                     EKQLYHEAVAEYQQAISLDPKYLEAHMSLGEIYEEKGLYQEAIARYNQVLGLDAQHPG
                     ATYGLALAYERVDPKKAIEQWERYIDLASTLSTEKDWVDIAKKHLEKLRRGDKVN"
     gene            3261..4277
                     /locus_tag="AC16G0_17685"
     CDS             3261..4277
                     /locus_tag="AC16G0_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5101490.1"
                     /translation="MHAEERAEQFRHAFTSLTGEIGKAVVGHQEIVRGVLICLFCGGH
                     ALLEGVPGLGKTLLVKTLAESLDLHFSRIQFTPDLMPADIIGTNIVVETPQGGRHFAF
                     QRGPLFGQIVLADEINRATPKTQSALLEAMQELAVTAGGVHHPLERPFFVLATQNPIE
                     MEGTYPLPEAQLDRFLLKLRVKYPSAADLAEIIERTTRPQAQQIQKVATSPEVETMKA
                     LVREVPIPQPVKEYVTQLVLSTRPEREGAPEMVRRFVRYGSSPRGAQALVLGAKVQAL
                     MDGRFNVSFEDVRQVALESLRHRLILSFEGEAEGIDPDQIIEDLLTRVPTSPAKVALL
                     PDDL"
     gene            4549..4920
                     /locus_tag="AC16G0_17690"
     CDS             4549..4920
                     /locus_tag="AC16G0_17690"
                     /inference="COORDINATES: protein motif:HMM:NF015834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HIR5101491.1"
                     /translation="MDERETFVRRTLAGTLVAGAAGVVVLLLLGWRAWALAFGLGSAI
                     SVGNFHLIVRAVRGMDTPGPGVSRHLWKGTLIRFVLVAAVLVLVVVVLRIDVLALVAG
                     LLVTQGIMVVYWLMWSLRTTE"
     gene            5010..5615
                     /gene="atpF"
                     /locus_tag="AC16G0_17695"
     CDS             5010..5615
                     /gene="atpF"
                     /locus_tag="AC16G0_17695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR5101492.1"
                     /translation="MRRLISLVRLSEFSLLCLVTALPAGVVLAAEEAARHEEPGIINL
                     NVTLLIQVVNFLILIALLSKFLFKPLTRFLAERAEGIEKSLTEARLAREAAAKAQEEY
                     RNQILTTQREAALLREQAQREVEAERQRLLQESREEAQRLVVDGKAQIEAETRRAKAS
                     LRQEVIGLSLLVAERILGRAMTADDQQRLAEQYLRDLGREN"
     gene            5612..>6106
                     /gene="atpH"
                     /locus_tag="AC16G0_17700"
     CDS             5612..>6106
                     /gene="atpH"
                     /locus_tag="AC16G0_17700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HIR5101493.1"
                     /translation="MIAGSLAKRYAKALVEVAAASDDLEAVRQELTDFAELLRVQREF
                     RIFVENPSVQGRDKASVLERIVAALGLRSLTSTFLRILLEGGRLGALEGILRAYVALM
                     DERLGRVRAMVTAATPLPAEQQERLRRRLAEVTGKQVYLEVHDDPSILGGLITQIGSL
                     VYDGS"
BASE COUNT         1044 a         1853 c         2162 g         1047 t
ORIGIN      
        1 ctgcgaggct acgtgcggga ccaggggggt ggtctggtga tgctgggtgg cgaggagagc
       61 ttcggcgtcg gagggtacta ccggaccccc atcgaggagg cgcttccggt gaccatggaa
      121 gcacgccaga aggtcgaagt cccctccttg gccgtgatcc tggtgattga tcggtccggc
      181 agcatggaga tgagcacgca agattctcgg ttcaccaaga tggatctggc caaggaggcg
      241 gcgcagctcg tggtggagct tctggatgag cggaacgagg tcggggtggt cgccttcgac
      301 accgcctggg actgggtggt ccccatcggc ccggccaaga acaaggatcg gatcctgaag
      361 gccatctcca ccatcaaggc gggcggcggt accgatctat tcccgccctt gaaggatgcc
      421 taccagacga tcttcgaccg gagggcgctc ctccgccacg ttctcgtcct gtccgatggc
      481 gaggtgcagg ccgcggattt tgcgggcctg gtgcgtcgga tgcagatgga caagatcacg
      541 gtctcctccg tggccatcgg cagtgacgcg gacgtgcgct tcatgacgga cctgtcccgc
      601 tggggacgag gccgcttcta ttacaccgac gatatctaca gcatcccccg gatcttcacc
      661 ctggagaccc agctggccag caaggcctcc atcatcgagg agcccttccg ggtgggggtg
      721 gcgagcgagg gtcatgagat cctccaggac atccgctggg atcaggcgcc gcctctgggc
      781 ggctacgtgt ccgccacgcc caagcctacg gcggatacgc tcctcctctc gcatcagcgg
      841 gaccccatct tggcgacgtg gcggtacggc ttgggaagga ccgcggcctt cacctccgac
      901 gccaagggga agtggggcat cctctggctg aagtgggggg actacgccaa gttcttcggt
      961 cagatggtcc ggtggacgct gcggacgacg cagcagcggg aggtggtgac ctccgtggtg
     1021 cagcgcgacg gccgggggga gatccaactg gaggcggtgg atcagaaggg ggatttcgtc
     1081 aacttcctgg aggcgaacgc cggagtgatc tccccggaca aggtccagcg ggtcctgccc
     1141 ctccagcaga tcggccctgg gcgctatcgc gggaccttcg acgccgccaa tgagggggcg
     1201 tatctggtag gggtagccga gcggaaggac cagaagatgg tcgggtccga agtggcctcc
     1261 ctggtgattc cctattcacc ggagctgcga gccctgtcgg tgaacgagcc tctgctccga
     1321 gacgtggcgg cactctctgg cggcgccgca ccctcccagc ccggggatgt gttcacccag
     1381 catcgtcggc cggcccgcgt gtgggtggag gcgtggccgc agctcctggc gctggcgctg
     1441 ttcctgttcc tgctggatgt ggccatccgg cgattctggc tacggggatg ggccctccgg
     1501 cgctcggccg gccgggatgg ggcgactagg tcgggtgagg ggactgggag ttgggggggc
     1561 gggtcagcct ccccgatggc cgcgcggttc gggagtcgtg gacgacggac gtagaccgcc
     1621 tcggcaaccc gcggcttccc tgtgagagca tgggggcgcg ttgagtgtgg catctcggca
     1681 acaacgaatg cgggggtatc ggcatgctgg cacacggggg gagaggggac gcacatgggg
     1741 tgacgaaaag gaacaggaat tcggggcgcg ccacggcact gatcctgctg atgggggccg
     1801 ttctgctgct gggcgctcct tccgggtggg ccggcgagca gacggcgacc gcttcatcga
     1861 aggtccggct cgcggtgatc ccgttcaccg gaccagccgc cggggtcccg gagggattcg
     1921 gggaggccct gggccaggcg atccgacacg ggctgtcgca ggtccgagcg gtccggccgg
     1981 tggactctgg cgagatcctt ggctcgagcc agcgcctgca tatctcgctg gccgatcggc
     2041 tgtcggacga ggcggccctc cgtctggtca aggacctcca ggcgcagggg ctggtgaccg
     2101 ggacctacgc cctggatggg gagtccctga gagtccaggc gcggctagct gatccaagcg
     2161 gggctggcca ggtcatcctg ggggaggaag ccattggacc cttggcccag ttcctgtcgg
     2221 cccagggccg gatcacgcgt cagatcctgc aggcgttccg tgtgcggctg acgccgtacg
     2281 acgagcggcg attgcaggcg gccttcagcg agcagaccgg ctcccttgaa gcctatgccc
     2341 tgtacgggcg ggccgggtgg cagcaggggc ttggcaccaa agagggatac gagcaggccg
     2401 tcgccctcct gagcaaggcg ctggagaccg accagaattt cgccctggcg cacttcgcct
     2461 taggcatctc gctccaggcg accaacaacc gatggaaggc ttcgggtgag tttcgcaaga
     2521 cgatccagct cgacgggacc ttcgcggacg cccacaagtg gctgggggac cttctggtca
     2581 agagcccgcg ccgtctatac gatcaggcga ttcaggcctt ccagaaggcc ctggagctgt
     2641 cgccagacta cgcggaggcc atggtcggcc tcggggatgc ccgccaggcg aaagggcaat
     2701 tcgacgaggc catcggggat tacaagcggg ccctgcagct cgagcccgag aacgcccgcg
     2761 tccacttcgg gctgggcaag atctattaca acgagaagca gctttaccac gaggccgtgg
     2821 ccgagtacca gcaggccatc agcctggacc ccaagtatct ggaggcgcac atgagcttgg
     2881 gggagattta cgaggagaaa ggcctgtatc aagaggccat cgcccggtac aaccaagtcc
     2941 tcggcctaga cgcccagcac cctggcgcga catacgggct ggccctggcc tatgagcggg
     3001 tcgacccgaa gaaggccatc gagcagtggg agcgatacat cgatctggcc tccacgctat
     3061 cgacggagaa ggactgggtg gatatcgcca agaaacattt ggagaagttg cgacgggggg
     3121 ataaggtcaa ctgagccaag gagcccctac gcggcagatg tgaatcagga aaggtgaggc
     3181 gggagcaggg gaagccgcag caaaccggga gtcggcaggc cgggacgtgc cgcccctaac
     3241 gagagaccaa cgagggaacc atgcacgctg aagagagggc cgaacagttt cgccatgcat
     3301 tcacttcgct caccggggag atcgggaagg ccgtggtggg ccaccaggag atcgttcgcg
     3361 gcgtcctgat ctgtctgttc tgcgggggcc acgccctgct ggaaggcgtg ccgggcctgg
     3421 ggaagacgct cttggtcaag acgctggcgg agagcctgga tctccacttc tcccggatcc
     3481 aattcacgcc cgatttgatg ccggcggaca tcatcgggac caacattgtg gttgagactc
     3541 cccagggtgg gcgacacttt gccttccagc gaggaccgct cttcgggcag atcgtcctgg
     3601 cggacgagat caaccgcgcc acccccaaga cccagtcggc cctgctggag gccatgcagg
     3661 agttggcggt cacggccggc ggcgttcacc accccctgga gcggccgttc ttcgtcctgg
     3721 ccacccagaa ccccatcgag atggagggga cgtacccgct gccggaggcc caattggatc
     3781 ggttcctgct caagctccgt gtgaagtatc cgagtgccgc ggacctggcg gaaatcatcg
     3841 agcggaccac ccggccgcag gcgcagcaga tccagaaggt ggctacgagc cccgaggtcg
     3901 agaccatgaa ggcgctggtg cgggaagtgc ccattcccca gccagtgaag gagtacgtga
     3961 cccagctcgt gctctccacg cgaccggagc gggagggggc cccagagatg gtccggcggt
     4021 tcgtgcgtta cggcagctcc ccgcggggcg cccaggcttt ggtcctgggg gcgaaggttc
     4081 aggcccttat ggatgggcga ttcaacgtga gctttgagga cgtgcggcag gtggcgctgg
     4141 agtcactgcg ccaccggttg attctctcct tcgaggggga ggcagaagga atcgatccgg
     4201 accagatcat cgaggacctc ttgacccgag tgccgacctc tccggccaaa gtggccctct
     4261 tgcctgacga cctgtagtcc taggagtcgc tgtcgggcgc ttcgagggtg cgtgcggcgt
     4321 gatgtatgtg accgtgcgtc gtccataaga gagccgccca gcgcttgacg ccgaggacca
     4381 cggggccatg cttgggtgtg tcaaaaagcc cttgacacct tttttcttcg cgtgttagct
     4441 tgagccgctc ttcgcgggtc ttgcggccgg aacgatgtct tcaggcgccc ggcttcccgg
     4501 agcggtgagc tggaacgagc ctctcagtcc ctctttccca tttcagtctt ggacgagcga
     4561 gagacctttg tccgtcgaac cctggccgga acgctcgtcg cgggcgcggc cggggtggtc
     4621 gtcctgctgc tcttgggttg gagggcttgg gcgctggcct tcggtttggg ttccgccatc
     4681 agcgtgggga acttccacct gatcgtccgc gccgtccggg gcatggacac gccgggtccg
     4741 ggggtaagcc gacacctctg gaaggggact ctgattcgct ttgtgctcgt tgcggcggtg
     4801 ttggtcctgg tggtcgtcgt gctccggatc gacgtgctgg cgctggtggc gggcctcttg
     4861 gtcacgcagg gcatcatggt cgtctattgg ctgatgtggt ctctccggac gaccgaatga
     4921 gtctcgtctg accgccgagg aacaccaagg aacactgcct gttcatgcgt cgatgggatc
     4981 acgggcatcc tgcggatgct ggaggcgcaa tgagacgcct gatttcgctc gtccgcctct
     5041 cggagttctc cctcctctgc ctggtcacgg ctctcccggc cggggtcgtg ctggcggcag
     5101 aagaagccgc gcggcacgag gaaccgggaa tcatcaatct gaacgtcacc ctgctcattc
     5161 aggtggtgaa cttcctcatt ctgatcgccc tcctgtccaa attcctcttc aagccgctga
     5221 cgcggttcct ggccgaacgc gcggagggga tcgagaaatc cctgacagag gctcgactcg
     5281 cccgggaggc cgccgccaag gcgcaggagg agtaccgaaa ccagattctg accacccagc
     5341 gcgaggcggc cctgctgcgt gaacaagccc agcgcgaggt ggaggcggag cgccaacgcc
     5401 tgctccagga gtcgcgggag gaggcccagc ggttggtggt cgatggcaag gcgcagatcg
     5461 aggcggagac gcggcgggcc aaggccagct tgcggcagga ggtgatcggc ctgtcgctcc
     5521 tggtggccga gcggatcctg ggccgggcca tgaccgccga cgaccaacag cggctggccg
     5581 agcagtacct gcgagatttg gggagggaga attgatcgcc gggtcactgg ccaagcgcta
     5641 cgccaaggcc ctggtggagg tcgccgccgc ctcggacgat ctggaggcgg tgcgccagga
     5701 gttgaccgac ttcgcagaac tcttgcgagt tcagcgcgag ttccggatct tcgtggagaa
     5761 ccccagcgtc caggggcgtg acaaggcatc ggtcttggag cggatcgtgg ccgcgctggg
     5821 cctccggtcg ctgacctcga ccttcctgcg aattctcctg gagggggggc gcctcggcgc
     5881 gctggagggc atccttcgcg cctacgttgc tctcatggac gaacgactcg ggcgggtcag
     5941 ggcaatggtg accgcagcca caccccttcc agccgaacag caagagcgcc tccggcggcg
     6001 cctggcggag gtcacgggga agcaggtgta cctggaagtc cacgacgatc cgagcatcct
     6061 gggtgggctg atcacccaga tcggcagcct cgtctacgat gggagc
//
LOCUS       DBNGZC010001028         2242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1159840, whole genome shotgun sequence.
ACCESSION   DBNGZC010001028 DBNGZC010000000
VERSION     DBNGZC010001028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1159840"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="AC16G0_17705"
     CDS             <1..870
                     /locus_tag="AC16G0_17705"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HIR5101494.1"
                     /translation="RPAPLRHAVRGGGGDSSWEALVKPAKPARVGRRLILAFGHLEVT
                     VEAQGIHGRRILRLPADVDLAAILHSYGVMPLPPYIRREAAAHEPRAETAIDGRSPGP
                     GGRGLDFERYQTVYAQEEGAVAAPTAGLHFTPELLHRIRQRGVLVHPVTLHVGPGTFQ
                     PVKVQTVSRHRMDPEHYTIPEETARAIRAAKEEGRRVIAVGTTSVRTLEHSAWQNHAV
                     RAEEAEADLFITPGYGFRVVDALITNFHLPRSTLLMLVAAFAGLELIRRAYAEAIAER
                     YRFYSYGDAMLIL"
     gene            907..2055
                     /gene="tgt"
                     /locus_tag="AC16G0_17710"
     CDS             907..2055
                     /gene="tgt"
                     /locus_tag="AC16G0_17710"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR5101495.1"
                     /translation="MKFELLITDQTSGARRGRLTTPHGRVETPVFMPCGTQATVKTLD
                     PRDLAAEGCELVLSNTYHLYLRPGHEVIQDLGGLHRFMAWDRAILTDSGGYQVLSLAA
                     LRRISEEGVAFQSHLDGSRHFLSPEKAMEIQLALGADILMALDECPPHDADPDYHRAS
                     MERTARWAARCRRAHPGDEPALFGIVQGGTHLDLRAQSAEETQRVGFTGYALGGLGIG
                     EGPAQMYAVAEHTTALLPADAPRYLMGVGTPRDLVECMARGVDMFDCVLPTRNARNGT
                     LFTGTGRLNIKGAAYARDERPVDPACACYTCQHFSRAYLRHLYVADEILGLRLNTIHN
                     LHYYVDLMQRARQAIESGTFSTWRAETLARMRPDPTTPNQTEEIQTHE"
     gene            2048..>2242
                     /gene="yajC"
                     /locus_tag="AC16G0_17715"
     CDS             2048..>2242
                     /gene="yajC"
                     /locus_tag="AC16G0_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR5101496.1"
                     /translation="MSELAYALGPQPGGGGAAGGGFSMLVPLLLMFAVFYFLLIRPQQ
                     KKQRTHQDMLKSLKVGDRIIT"
BASE COUNT          381 a          758 c          735 g          368 t
ORIGIN      
        1 cggccggccc cgctccgcca cgcggtccgg ggcggaggcg gcgactcgtc ctgggaagcg
       61 ctggtcaagc ccgcaaagcc ggcccgggtg ggccggcggc tgattctggc tttcggacat
      121 ctggaggtga cggtggaggc gcaggggatc cacggccgcc ggatcctgcg ccttccggcc
      181 gacgtggacc tggctgcgat cctgcactcc tatggcgtca tgccgctgcc gccgtacatc
      241 aggcgggaag ccgcagctca cgagccgcga gccgagacgg ccattgacgg tcgcagcccg
      301 gggcccggag gtcgggggtt ggatttcgag cggtaccaga cggtgtatgc gcaggaggag
      361 ggcgccgtgg ctgcgcccac cgcgggcctg catttcacac cggagctgct tcatcggatt
      421 cgccagcggg gcgtcctggt gcatcccgtg accttgcatg tcgggccagg gacttttcag
      481 cccgtcaagg tgcagacagt gtcccgccac cggatggacc cggagcacta taccatcccg
      541 gaggagaccg cccgggccat tcgggcggcc aaggaagaag gccggcgagt gatcgccgtg
      601 ggaacgacca gtgtgcgcac cctcgagcac agcgcatggc aaaatcacgc cgtccgggca
      661 gaggaggcgg aggcggacct cttcatcact cccggctacg gctttcgcgt ggtggacgcc
      721 ctgatcacca acttccacct gccccgctcc acgctcctca tgctggtcgc cgcctttgcc
      781 ggtctggaac tcatccgtcg agcctacgcc gaggccatcg cggagcgcta ccgcttttat
      841 agctatggcg atgccatgct gatcctttga tgggtccggc catgtgtcgg atcagtccgg
      901 gtggcgatga aatttgagct cctcataacg gatcagacga gcggcgcccg ccgcggacgg
      961 ctgaccaccc cccatggcag ggtggagacc ccagtcttca tgccctgcgg gacccaggcc
     1021 acggtcaaga ccctggaccc gcgcgatctc gccgcggagg ggtgcgagct ggtcctgagc
     1081 aacacctacc atctgtatct tcggccggga cacgaggtca tccaagacct cggtggcctg
     1141 caccgcttca tggcatggga ccgggccatc ttgaccgaca gcgggggcta ccaggtcctc
     1201 agtctggccg ccctgcggag gatcagcgag gaaggggtcg cgtttcagtc ccacctggat
     1261 ggctcccgcc acttcctgag ccccgaaaag gccatggaga tccagctcgc cctcggcgcg
     1321 gatatcctca tggcgctgga cgaatgcccg cctcacgacg cggatcctga ctatcaccgc
     1381 gcatccatgg agcgtaccgc tcgctgggcg gcgcggtgcc gccgggctca cccaggcgat
     1441 gagcccgccc tcttcgggat cgttcagggg ggcacccatc tggacctccg ggcccaatcc
     1501 gccgaggaga cgcagcgggt tggcttcacg ggttacgccc tggggggcct gggaatcggc
     1561 gaagggccgg cacagatgta cgcggtcgcg gagcatacca ccgccctgct cccggcggac
     1621 gccccgcgtt acctgatggg cgtgggaacg ccccgagacc tcgtggagtg catggctcgc
     1681 ggcgtggaca tgttcgactg cgtcctgccg acgcggaacg cgcgaaatgg aacgctcttc
     1741 accggaaccg gccgactcaa catcaagggg gccgcctacg cacgagatga gcgaccggtg
     1801 gatcctgcct gtgcctgcta tacgtgccaa cacttttccc gggcctacct gcgccacttg
     1861 tacgtagccg acgagatcct cggtcttcgc ttgaacacca tacacaatct gcactactat
     1921 gtagacctga tgcagcgcgc ccgccaggcc atagagtccg ggacattctc gacctggcgg
     1981 gcggagaccc tggcacggat gcggcccgac cccacaaccc cgaaccagac agaggagatc
     2041 cagacccatg agtgagctcg cctatgcctt gggaccccag ccgggaggtg gcggcgcggc
     2101 cggtggcgga ttcagcatgc tcgtgccgct gctcctgatg ttcgccgtct tctatttcct
     2161 tctcatccgg ccgcagcaga agaagcaacg gacgcaccag gacatgttga agagtctgaa
     2221 ggtcggcgat cggatcatca cc
//
LOCUS       DBNGZC010001029         2104 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_675310, whole genome shotgun sequence.
ACCESSION   DBNGZC010001029 DBNGZC010000000
VERSION     DBNGZC010001029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_675310"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            534..1199
                     /locus_tag="AC16G0_17720"
     CDS             534..1199
                     /locus_tag="AC16G0_17720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101497.1"
                     /translation="MSEWKEGGLGQRPEAEERGKSRSRASACLLAVLGLTAVLASACV
                     SRVATPRPWQDFGTTSVGSLRGELSRLSAPEATERWTYFAVGAAEDHRSRGDMCERGY
                     SLPEFVAYLQYTAKDDQLLSEALSEFWTNHHCRRQIQKVEPPVPSRNGGSEAWESAGR
                     LLLAVRQSEDITLLQVLVREAETHPADPVALTKGLVARARLEEMTRRGVQSPFRVSVP
                     ALR"
     gene            complement(1577..1831)
                     /locus_tag="AC16G0_17725"
     CDS             complement(1577..1831)
                     /locus_tag="AC16G0_17725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101498.1"
                     /translation="MARSKRPYVCLPCWDENGKTGVPATHRLLVREVGEKWGDEVWGC
                     RVHIAELSAHLLGSGKPLDLIVHNMMTGACVIEAHPRPVG"
BASE COUNT          372 a          687 c          668 g          377 t
ORIGIN      
        1 gtctgccggt ggttctcgtc caagaagctc acgacctgtg gctcgatcca agtatccggg
       61 aagaagcacg attgccgccc ctcctgcgtc catcttcagc cgatgggatg gtcgcgcaac
      121 ttggcagcac gcgggtcaaa tgcccggcga atcagacccc tgagttctgc ggcacgttgt
      181 tcaggaagga gaataactgt gccgcggtcg ctctgcactg cggtgcgtcg gcaccttgcc
      241 taccatccac tgacgcctcc cggcctctcc gttgccaaga cggcgcgcca gctcggcgtc
      301 tcggccccgg ggatcgcaat ggccgtcgcc aggacgaacg cgagatatgt ccacttcgtc
      361 agctacgggc gtgttccgca ttcgtccacg gtgacgttcc gccagcgctc ggcctctgcg
      421 agccagcgac tccaccgcct gaattcgctt gacagggtgc tgtctgttcg gcagccagct
      481 tccccaaccc tcttcccgac tgcgcctgga gcgtggagcg aaaggaggcc aggatgagtg
      541 agtggaagga aggcggcctg gggcaacggc cggaggccga ggagcggggc aagtcccggt
      601 cgagggcgtc agcctgtctc ctggcagtgc tcgggctgac ggcggtgctc gcttcggcat
      661 gtgttagccg ggtcgcgacc ccccggccgt ggcaagactt cgggacgacc tcagttggaa
      721 gtctgcgcgg ggagctttct cgcctatccg cgcctgaggc cacggagcgc tggacgtact
      781 tcgcggtcgg ggccgcggaa gatcaccgct cccgaggcga catgtgcgag cggggatata
      841 gcctgccgga gttcgtcgcc tacctgcaat acacggcgaa ggatgaccaa ctgttgtccg
      901 aggcgctttc ggaattctgg acaaatcacc actgtcggcg tcagatccag aaggtcgagc
      961 cgcccgtccc cagcaggaac ggagggagcg aggcgtggga gtccgcgggc cgtttgctct
     1021 tggcggtccg ccagagcgag gatatcaccc tcctgcaggt cctggtccgg gaggctgaga
     1081 cgcatccggc cgaccccgtc gccctgacga aaggcctcgt ggcccgcgcc cggctcgaag
     1141 aaatgactcg ccgcggggtg cagtctccct tcagggtgtc agtcccggcg ctcaggtgaa
     1201 cgtggagcca gcagtgcccc tgaacggcga cggtagttgg gtgtttgatg gcgctcgcgt
     1261 acctgaggat tgtgtgtcgc tgacgtggtc tgcggtgaag gcacgccctc aacttgccca
     1321 ggtcatccga ttggaccagc cggaagcggg ggaaaatcga tagcggagtc gcccatggag
     1381 cacctggggg cgggcgaggc ttgtgccgag atgtctatcc tcgccaaggc tgacgacatt
     1441 acgcgactcg acccacgtcc agagtaagag cggcaggaac atcaacggct cgcggctatt
     1501 cacctcgccg ccacatccaa aaccttagtc tcgggcaact tccccgtccc gtgggcatgt
     1561 ctccctgggt cctccctcac cccaccggcc tcgggtgcgc ctcgatcaca cacgccccgg
     1621 tcatcatgtt gtgcacgatc aggtcaagtg gcttccccga cccgaggagg tgcgcggaca
     1681 gctcggcaat gtgcactcgg catccccaca cctcatcccc ccatttctcc ccgacctctc
     1741 gcaccaaaag tcggtgggtg gcagggaccc ctgttttgcc gttctcatcc caacacggca
     1801 ggcatacata ggggcgtttc gatcgcgcca tccctgtgac ctcgttcccc tgagcatggc
     1861 ccctcgcgag aggggtagga ccaggacgtt ccgcagccat gcctggcggg ggataccacg
     1921 cggctatacg tagttgagta tttcagccaa tgcgctacct atcctccgaa gcagaaaccc
     1981 gacgagggcc ccaagaaaaa gtcgacgatg gccccacgcc ctactttgct caatccaaac
     2041 gtcggcccac cgtggagtgg accggagcgg agcgagtcaa gggcaagcgg gtgttagccc
     2101 ttgt
//
LOCUS       DBNGZC010001030         2065 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1750291, whole genome shotgun sequence.
ACCESSION   DBNGZC010001030 DBNGZC010000000
VERSION     DBNGZC010001030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1750291"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="AC16G0_17730"
     CDS             complement(<1..1173)
                     /locus_tag="AC16G0_17730"
                     /inference="COORDINATES: protein motif:HMM:NF024867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation SMC family protein"
                     /protein_id="HIR5101499.1"
                     /translation="MQLSKISLFGFKSFADKTELTFEPGITVVVGPNGCGKSNVSDAI
                     RWALGEQSAKLLRGDRMDDLIFAGNARRKPIGLAEVSLTLTRNEGAIPTEYEEVNVTR
                     RLYRSGESEYLLNKVPCRLREITDLFIDTGLAGEPYALIEQGSIGSVVNARPADRRVL
                     IEEAAGIMKYKTKKRAAVAKLEATEQNLLRLRDVIVEVERQRNSLKRQANKAERYKQL
                     DLRATHLKLYLKYREHTALWEELQSLLAQLRPLHEVQIGLRAGIAASETALEERRLQA
                     LSQEQAVAAAQEAVYALRGQIDRDEAELRNLGQQIEAAEARRTETAASLGSLGDTVRR
                     LLGELEGGSSRVAVQEREVASLEAGLEAEGQTLHEVESGLATGVAELDRLRHQAAHQ"
     gene            complement(1272..2000)
                     /locus_tag="AC16G0_17735"
     CDS             complement(1272..2000)
                     /locus_tag="AC16G0_17735"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:NF025973.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III family protein"
                     /protein_id="HIR5101500.1"
                     /translation="MDDRLAAFRAALNYRFRNASLLSLALSPLQPPLTPEAAAARQRL
                     EFLGDAAWNFSVAAAVFQVHLGATAGDLTRLRAAWCSTSGLAQLARQFGLPAPEDPVS
                     LAPSDRVMAEMLEAVLGAMVEDGGLEAVQSLALHVVMREGLAMVPPPLDPKSALQLLA
                     QARHLRLPAYRLLERRGPSHHPTFRVQVTLRDQGVDVQADAEGNSRQSAEQEAARLAL
                     LKLAEASESRPQHLVPDLPPGPQP"
BASE COUNT          354 a          644 c          700 g          367 t
ORIGIN      
        1 ctggtgcgcg gcctgatgtc gcaggcgatc cagctcggcc acaccggttg ccaggccgct
       61 ctcgacttcg tggagcgtct gtccctcggc ctcgaggccg gcttccagag atgccacctc
      121 ccgctcctgg accgccacgc gggaggagcc tccttccagt tcccccagca ggcggcggac
      181 ggtgtccccg agggatccga gggacgcggc ggtctcggtc cggcgggcct cggcggcctc
      241 gatctgctgg ccgagattcc gcagctcggc ctcgtcacga tcgatctgtc cgcgcagggc
      301 gtagacggct tcctgggctg cggccaccgc ctgctcctgg ctgagggcct ggagacggcg
      361 ctcctccagc gctgtctcac tggcggcgat gccggcgcgc aggccgatct ggacctcgtg
      421 caagggacga agctgggcca gaagcgattg aagctcctcc cagagcgccg tgtgctcgcg
      481 atatttgagg tacagcttca ggtgcgtggc gcgaaggtcg agctgcttgt accgctccgc
      541 cttgttcgcc tgtcgcttca gagaattccg ctggcgttcg acctcgacga tcacgtcgcg
      601 gaggcgcagg aggttttgtt ccgttgcctc cagcttggcc accgccgcgc gcttctttgt
      661 cttgtacttc atgatccccg ccgcttcctc gatgagaacc cggcggtcgg cgggtctggc
      721 gttgacgacg ctgccgatgc ttccctgctc gatgagcgcg taaggttccc cggccaggcc
      781 ggtatcaatg aacaagtcgg tgatctcgcg gagacggcac gggaccttgt tcagcaagta
      841 ttcgctctcg ccggagcggt agagccgccg ggtgacgttc acctcctcgt attcagtagg
      901 gatcgcaccc tcgttgcgcg tgagggtgag cgatacctcg gccaggccga tcggcttgcg
      961 ccgggcgttc ccggcaaaga tcagatcgtc catccggtcg ccgcggagaa gcttggcgct
     1021 ctgctcgccc aaggcccaac gaatcgcgtc cgagacatta ctctttccgc acccgttggg
     1081 cccgacgacg acggtgatgc ccggctcgaa tgtgagttcc gtcttgtccg cgaaagattt
     1141 gaaaccgaaa agggagattt tagagagttg catagatgtc tctcacccca ggagtctcgt
     1201 caacggggaa aaggccctta agatttcgcg aacgctacca tgaggcggaa gttgatgtca
     1261 agctggaaat actacggttg ggggcctgga ggtagatctg gtacgagatg ttgtggccga
     1321 gactcggagg cttccgccag tttgaggagc gccagacgag ccgcctcctg ctctgcggat
     1381 tggcggctat tgccttcggc atccgcctgg acatccacgc cttggtcccg aagcgtaacc
     1441 tggacgcgaa aggtcgggtg atgggacggc cctcgccgct cgagaagtcg gtaggccggt
     1501 aaccggaggt gacgagcctg cgccaagagt tgcagggcgg actttggatc caggggcggg
     1561 ggaaccatgg cgaggccctc ccgcatgacg acatgcaggg ccagagactg tacggcctcg
     1621 agccctccgt cctctaccat ggcgcccagg accgcttcca gcatttcggc catgacccgg
     1681 tcgctcgggg cgagagagac cgggtcctcc ggggcgggaa gcccaaactg ccgggcgagc
     1741 tgtgccagac cggaggtgct gcaccacgca gctcggagtc gagtgaggtc gccggcggtt
     1801 gcgcccagat ggacctgaaa gacagccgcg gccacagaga agttccaggc ggcatccccc
     1861 agaaactcca accgctgccg tgcggccgcc gcctcaggcg tgaggggggg ctgaagcggg
     1921 ctcagggcca gcgaaagcag cgacgcattt cggaagcggt agttcagggc tgcccggaag
     1981 gcggccagcc ggtcatccat ggggaacgca ggaggatcag gaggagacat gaaaccaaag
     2041 ttaacacggg gcctggggag ggtca
//
LOCUS       DBNGZC010001031         1820 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2144849, whole genome shotgun sequence.
ACCESSION   DBNGZC010001031 DBNGZC010000000
VERSION     DBNGZC010001031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2144849"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..789)
                     /locus_tag="AC16G0_17740"
     CDS             complement(172..789)
                     /locus_tag="AC16G0_17740"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR5101501.1"
                     /translation="MPDLTGLIVHWGYLAIFLFVVLGNMGLPVPEESILILSGYLVWQ
                     QDLRLPIVLVVGVLSAMAGDNLGYWLGRRYGQEAIERYGRWILATPKRLEATRRFVTR
                     YGAIGVFSARFIAGLRFLAGPLAGSTGLRPAVFFVANALGALIYVPTMVGAGYAVGYG
                     LGDYVKQLQRMVGRIEHVVLVAAILSTLVLLGWRALQASRPRKHP"
     gene            complement(1169..>1820)
                     /locus_tag="AC16G0_17745"
     CDS             complement(1169..>1820)
                     /locus_tag="AC16G0_17745"
                     /inference="COORDINATES: protein motif:HMM:NF052145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA-type ATPase lid domain-containing protein"
                     /protein_id="HIR5101502.1"
                     /translation="GHMDHVLQVKLLKAIEEKAVRRVGGLRSKPFNARIVAATNRDLD
                     AAIAGGTFRPDLYYRIKALTIEVPPLRERGKDTLLLARDFLERFTRQYGLPQATLAPE
                     AEELVFEYPWPGNVRELAHVIERAVLLHGGPVLQAEQLGLAVEKAKALLVVKPGAPVQ
                     VDFSNGGIVLEDVERHLITEALQVAGWNRGRAAQLLGISKETLRYRIEKHQLQATA"
BASE COUNT          323 a          575 c          618 g          304 t
ORIGIN      
        1 agtacccgcc cccctttggc atgctgctcc ggcagaccgc ccgactcatc aagactggcg
       61 ttgcgggtgg acgtcttcgg cgattacagc atggagcgtg gccacgccga tgacatgctc
      121 cagccgggcg ggccggagag gaacgggacg gcatgggggg gtggtcgtgg ttcagggatg
      181 ctttcttggg cgcgaggcct gcagcgcccg ccacccgagg aggaccaggg tcgagaggat
      241 cgccgcgacc aggacgacat gctcgattcg gcccaccatg cgctgcaact gcttcacata
      301 gtcacccagc ccatacccga cggcatagcc cgccccgacc attgtcggca cgtagataag
      361 ggcgccgagc gcgttggcca cgaagaagac ggcgggccga agacccgtgc tgcccgccag
      421 gggccccgcc aggaagcgaa gtccggcgat gaagcgcgcg gagaagaccc cgatggcccc
      481 gtagcgcgtc acgaagcgac gtgtggcttc gagtcgtttg ggcgttgcca ggatccatcg
      541 gccgtaccgc tcgatggcct cctggccgta tcgtcgcccg agccagtacc cgagattgtc
      601 gcccgccatg gcgctgagga cgccgacgac caggacaatc ggcaatctga gatcctgttg
      661 ccataccagg tagccggaca ggatgaggat cgactcttca ggaacgggga gccccatgtt
      721 cccgaggaca acgaagagga agatggcaag gtagccccag tgaacaatca acccggtgag
      781 gtcaggcatg tctgttgtcg cttgcggccg gagattcagc tcggaagaca cctttgatcc
      841 gctcgcgcct gcctcgcttg ctccccatta ccagcgggcc agccaagagg tggcgccggg
      901 gtgcgtggcg actggagaag tgtacgccca ccggcgccgg ttgtccacga gttcgcggga
      961 gagtgaggaa ctcgtcttcg cccaggctgc tcaagaggat ccgggtgcag ggcggcgcac
     1021 acatctatcc ttgggtgccc ggtgtggggt agccacccgg aaggcgggac tccacacatg
     1081 ggccctgctt cagcagcctg ctagcgggag aaccgaagtc gttgcggcga tgggggcttt
     1141 gtgcgggggc cagggtgggg ctcgggtctc aggccgtggc ctggagctga tgcttttcta
     1201 tccggtaccg aagggtctcc ttggagatcc ccaggagttg tgctgcccgg cctcggttcc
     1261 agccagcgac ctggagggcc tccgtgatga gatgtcgctc gacatcttcc agcacgatac
     1321 cgccgttcga gaagtccacc tgcaccggtg caccaggctt gaccacgagc agagccttgg
     1381 ctttttccac ggcgagcccc agctgctcgg cctgcaacac gggacctccg tggagaagaa
     1441 cggctcgctc aatgacgtgc gccagctcgc ggacgttccc cggccacggg tactcgaaga
     1501 ccaattcctc cgcctcgggg gcgagggtcg cctggggaag gccgtactgc cgcgtgaatc
     1561 gttcgaggaa gtcccgagcc agcagcaggg tgtccttccc ccgctcgcga agtggaggga
     1621 cctcgatggt caaggccttg atccggtagt agagatcggg acggaaggtc ccgcctgcga
     1681 tggcagcatc cagatcccgg ttcgtcgcgg cgacgatgcg ggcgttgaag ggcttcgacc
     1741 gaagtccccc cacccggcgc actgccttct cctcgatcgc cttgaggagc ttcacctgga
     1801 ggacgtggtc catgtggccg
//
LOCUS       DBNGZC010001032         5109 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1852210, whole genome shotgun sequence.
ACCESSION   DBNGZC010001032 DBNGZC010000000
VERSION     DBNGZC010001032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5109)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5109)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5109
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1852210"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /gene="epmA"
                     /locus_tag="AC16G0_17750"
     CDS             <1..942
                     /gene="epmA"
                     /locus_tag="AC16G0_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HIR5101503.1"
                     /translation="RGFVEVETPCLQVSPGLDSGIQALVTEVAGPLGRNRRRLYLHTS
                     PEFAMKKLLVAGVERLFQIAHVWRDGERSPLHHPEFMLLEWYRAHVSYEVLMEDCEGL
                     LKASAAAAGVVELRHGATRCDPTAPARRLTVAEAFARYCGIDLLATTPDPHAPDTTRL
                     AAEARRIGLHTGAGDRWEDVFHRILMDRIEPHLGDGAPTILCEYPVSRAALSRPSPRD
                     PRVAERFELYVCGLELANAYSELTDPVEQRRRFEADQALLERRYGARYPIDEDFLAAL
                     DAGMPESAGIALGLDRLVMLLAGADRIDDVLWAPVAE"
     gene            1230..2324
                     /locus_tag="AC16G0_17755"
                     /pseudo
     CDS             1230..2324
                     /locus_tag="AC16G0_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002632369.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
     gene            2553..3575
                     /gene="pdhA"
                     /locus_tag="AC16G0_17760"
     CDS             2553..3575
                     /gene="pdhA"
                     /locus_tag="AC16G0_17760"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HIR5101504.1"
                     /translation="MAKRPNKATVESAPEVARKPPMDKKVMMDLLYQMLLVRRFEEKA
                     AEMYAQGKIGGFLHLDVGMEASCVGAMSVLRPDDYAIAGYREHGHALVKGSDARRAMA
                     ELFGRVDGLSKGKGGSMHFFDKEHNFMGGTGIVGGQLPIGTGIGFAINYQGGDQVCLC
                     FFGDGSVAQGVFHESLNMAALWKLPVIYICENNLYAMGTALARTQSVTDIYKKAEAYG
                     IPGEQVDGMDVLAVREATIRAVERARREKIPTLLETRTYRFRGHSMRDPAAALYRTRE
                     EVEEWKKSDPIVLLWDKLSSEGIISDAEYKEMDKEIAQVVEEAVRFADASPQPPLSEL
                     LTDVYV"
     gene            3651..4628
                     /locus_tag="AC16G0_17765"
     CDS             3651..4628
                     /locus_tag="AC16G0_17765"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HIR5101505.1"
                     /translation="MAIITYREALNQALREEMRRDPRVFLMGEEVGVYQGAYKVSQNL
                     LEEFGPKRVIDTPICEAGFVGVGIGSAMAGLRPVVEIMTFNFAIVALDQIVNHAAKIL
                     YMSGGQFKVPMVIRGPGGPAVQLAAQHSQSMESYFYHVPGLMVVRPSTPRDAKGLLKS
                     AIRDDNPVIFIESETLYGLKGEVPDEEYTIPLGVAEVKREGTDVTVIGHMGMLHRALE
                     VAADLEKEGISVEVVDPRTLRPMDTSTILTSVKKTHHAVVLEAGAGFAGLGAEIATLI
                     SEGAFDFLDSPVVRVNGANCPMPYARNLEQAKTPDKARIIAGIKQALSV"
     gene            4852..>5109
                     /locus_tag="AC16G0_17770"
     CDS             4852..>5109
                     /locus_tag="AC16G0_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2118 domain-containing protein"
                     /protein_id="HIR5101506.1"
                     /translation="MPSQVIMPKLSDAMVEGKVLQWMKKEGDRVQGGDVLASIETDKA
                     EIELEAFGSGVVRKILVDEGQAVPVGQLIAVIAEPDDDISEL"
BASE COUNT          956 a         1519 c         1738 g          896 t
ORIGIN      
        1 cgcggcttcg tcgaggtgga gacaccttgc ctccaggttt cgccgggtct ggactcgggc
       61 atccaggcgc ttgtgaccga agtcgccggc cccctcggcc gcaaccggcg gcgactttac
      121 ctgcacacct cgccggagtt cgccatgaag aagctcttgg tggcgggtgt cgagcgcctc
      181 ttccagatcg cccatgtctg gcgcgacggt gagcgctccc ctctccatca cccggagttc
      241 atgctcctcg aatggtatcg ggcgcatgtg tcatacgagg tgctgatgga ggactgcgag
      301 gggcttctga aggctagcgc cgctgctgcg ggtgtcgtgg agttgcggca cggtgccacg
      361 cgctgcgacc ccacggctcc cgcccggagg ctgacggtcg ccgaggcttt tgcccgctat
      421 tgcggcatcg acctcctagc gaccaccccc gatcctcacg cacccgacac tactcggctt
      481 gccgcggagg cgcgacgaat tggcctccac acgggagcgg gggacaggtg ggaggatgta
      541 ttccaccgca tcctgatgga tcgcatcgaa ccccacctcg gcgatggcgc ccccacgatc
      601 ctgtgcgaat atcctgtgtc gcgggcggca ttgtcgcgcc cgagtccacg tgacccgcgg
      661 gttgccgagc gcttcgagct gtatgtctgt ggcctcgagc tggcgaatgc ctacagcgag
      721 ctgacggacc ccgtcgagca gcgccgccgc tttgaggccg atcaggcgct gctggaacgg
      781 cgctacggtg cgcgctaccc catcgacgag gacttcctgg cggcgcttga tgccgggatg
      841 cccgagagcg ccggcattgc cctgggcctg gaccggctgg tgatgctctt ggccggggca
      901 gacaggatcg atgatgtgct ctgggccccg gtggcggagt gagccagtgc agtgaagaaa
      961 aacgatcgcc gggctcaccc tttggtgggg ggtatccggg tgggtggtga gcctgagcga
     1021 tccgtcggaa acggaacacc aagaggggga gccaggggtc ggagggggca aggcgtccag
     1081 gccaaatcct cattggggtt gaattccagg ggtacgtatc ttaaggttaa accatacacg
     1141 gctggctccc cgcctctttt gtctccgatg tcggggacgt gacgggctgc gagacgactc
     1201 gggcgaaggc agggaaagga ttgtcgcgaa tgatcacgga acaggccgtg cttcaagcct
     1261 tatctacagt gaaggacccg gagatccacc gcgacttggt atccctggga atgatcaaag
     1321 gcctcgagat cgacggcccg aaggtgagct tcgaggtcgt cctgaccacg cccgcgtgcc
     1381 cgctcaagga gcagatcgag cacgactgcc gcctggcatt ggacgctatc ccaggcatcg
     1441 gccaggtggc catcaggtgg ggggcgagcg tcgccgcccc gcgcggggtc gtggatagga
     1501 gcggcatccc gggcgtcaaa aacatcattg ccgtggcgtc gggaaagggt ggggtgggca
     1561 aatccacggt cagcgtgaat ttggccgtgg ccctcgccga aaccggcgcc aaagtgggac
     1621 tgctggatgc ggacgtctat gggcccagca tccccttgat gatggggatc caccgattac
     1681 ccgacctcaa ctcggagcag cgcatcatcc cgctggaagc gcacgggatc aagctgatgt
     1741 cccttggttt catgtaacca gaccacaccg ctcctgtggt ctggcggggg ccgatgctct
     1801 ccaaggccct tacgcagttt ttccgggacg tggcctgggg ggagttggac tatctgctgg
     1861 tggatctgcc gccggggacc ggggatgcac agctcacgct gtcgcaatcg ctgaacctct
     1921 caggagttgt gatcgtcatg acaccccagg atgtggccag gaccatcgcc agccgggtgc
     1981 tggtgatgtt ccgccagatg aaggtgccga tcttggggat cgtggagaac atgagcagct
     2041 ttgtctgtcc tacgtgtcac aaagagagtc agatcttcag tcacggggga ggtcggaagg
     2101 ccagcgagcg actggaggtc ccgttcctgg gggagattcc tctggacggc gacctgtgcg
     2161 agggtgggga cgacggccga ccgatcctgg tgcgaaatcc cgggtcgccg gtggcggagg
     2221 tcttccgccg cttggcggga agtctcgccg gacgggtgag cgtcgaagcc gtgaggtcgc
     2281 aaggcgggtt cggtcagccc cttcccatca tcagagtcgg gtagacgtcc gaggagggcc
     2341 agggccaacc cggcggggat gcgcgggtcc ccccggagac gtgtaaaacc aagacacgac
     2401 gtggtgaatc gggaaagcct gcgacaggcg gcaagaggtc agaattcggc taaatacatt
     2461 tgcaagcgca acctttttgg ctatactgcc cgccgatgtc cacacccgtc ggaaacagcc
     2521 cgcccccaat gaacccgcag aaggaaccgg tcatggcaaa gcgtccaaat aaagccacag
     2581 tcgaatcggc ccccgaggtc gcgaggaaac cgccgatgga caagaaggtc atgatggatc
     2641 ttctgtatca gatgctcctc gtccgccgct tcgaggagaa agcggcggag atgtacgctc
     2701 agggcaagat cggcggcttc ctgcacctag acgtgggcat ggaggcctcc tgcgtagggg
     2761 ccatgtcggt cctgcgcccc gacgactacg cgattgccgg atatcgcgag cacggccatg
     2821 ccctggtcaa gggcagcgat gcccggcgcg ccatggcgga gctgttcggc cgagttgacg
     2881 gtctgtcgaa ggggaaaggc ggctccatgc acttcttcga caaggaacac aacttcatgg
     2941 gcggcaccgg gatcgtgggc ggtcaactgc ccatcgggac cggcatcggc ttcgccatca
     3001 actaccaggg cggcgaccag gtttgcctat gctttttcgg ggacgggtcg gtggcgcaag
     3061 gcgtgttcca cgagtccctg aacatggcag cgctgtggaa actgccggtg atctacatct
     3121 gcgagaacaa cctgtacgcc atggggaccg cgctggcccg cacccagtcg gtgaccgaca
     3181 tttacaagaa ggcggaagcc tacgggatcc ccggcgagca ggtggacggc atggatgtct
     3241 tggcggttcg cgaggccacc atccgggccg tggagcgcgc ccgccgcgag aagatcccca
     3301 ccctcctcga gacccggact taccggttcc gcggtcactc catgcgggac ccggcggctg
     3361 cactctatcg cacgcgcgaa gaggtggagg agtggaagaa gtcggacccc atcgtcctcc
     3421 tgtgggataa gctgagcagc gagggaatca tcagcgacgc cgaatacaaa gagatggaca
     3481 aggagatcgc ccaggtggtg gaggaggcgg ttcgattcgc cgatgcgtcc ccccagccgc
     3541 cgctctccga actcctcacc gacgtgtacg tctaagacgc ccacagccga caggaacagc
     3601 gactgccaag ggtgggccct gtcggaactc gttgtcggag gtcgccgggc atggccatca
     3661 tcacgtatcg cgaggcattg aatcaggcac tgcgggagga aatgcggcgg gacccgcggg
     3721 tgttcctcat gggcgaagag gtcggggtct accagggcgc ctacaaggta agccagaatc
     3781 ttttggagga gttcgggccg aagcgggtga ttgacacccc catctgcgaa gcgggtttcg
     3841 tcggcgtggg gatcggatcc gccatggccg gccttcgccc ggttgtggag atcatgacat
     3901 tcaacttcgc catcgtcgcg ctggatcaga tcgtgaacca cgcggcgaag atcctgtaca
     3961 tgtccggcgg gcagttcaag gtccccatgg tgatccgggg gccgggcgga ccggcggtgc
     4021 aactcgcagc gcagcactct caaagcatgg agagctactt ctaccacgtt ccggggctga
     4081 tggtcgttcg cccctcgacg cccagggatg ccaagggcct cttgaagagc gccatccggg
     4141 acgacaaccc cgtgatcttc atcgagtcgg agacccttta cggactgaag ggggaggtcc
     4201 cggacgaaga gtacacgatc ccgctgggtg tggccgaggt gaagcgggag gggaccgacg
     4261 tcacggtgat cggacacatg gggatgcttc accgcgcgct ggaggtcgcc gcagacctgg
     4321 agaaggaagg catctcggtg gaggtggtgg acccgcggac gctccggccc atggatactt
     4381 cgacgatcct gacctcggtg aagaagacgc atcacgctgt ggtcctggag gccggcgcgg
     4441 gctttgccgg cctgggggcg gagatcgcca ccctcatctc cgagggggct tttgattttc
     4501 tggactcgcc ggtggtgcgt gtgaacgggg caaactgccc catgccctac gccaggaacc
     4561 tggagcaggc caagaccccg gacaaagccc gcatcatcgc gggcatcaag caggccctgt
     4621 ctgtctagtg cagcagggcg agcgaacgtc tatccacgca tccgcgccgc tgaatagtct
     4681 ttgggtgcct ccaggatttg cgccggccgc tcgtctcggg cgtacggagg ctctcaaaca
     4741 gatatcgaag aacagacgaa gccgtccggg ttgatccggc aatcgccgcg gctgcccggt
     4801 ctttcgtcgt cactcgtcgg tttcctattt tccactagag cggagctgcc catgccatcc
     4861 caagtcatca tgccgaagct cagcgacgcc atggttgagg gaaaagttct gcagtggatg
     4921 aagaaagaag gggatcgcgt tcaggggggc gacgtgctgg cttctatcga gacggacaag
     4981 gctgagatcg agctggaggc cttcggctcc ggcgtcgtcc ggaagatcct ggtggatgag
     5041 ggccaggctg taccggtggg acagctcatc gccgtcatcg ccgagccgga tgatgacatc
     5101 agcgagttg
//
LOCUS       DBNGZC010001033         2561 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2240896, whole genome shotgun sequence.
ACCESSION   DBNGZC010001033 DBNGZC010000000
VERSION     DBNGZC010001033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2240896"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="AC16G0_17775"
     CDS             complement(<1..786)
                     /locus_tag="AC16G0_17775"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017952515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR5101507.1"
                     /translation="MLDKFHEACGVFGILGSGEAANLTYLGLYALQHRGQESAGIVTS
                     DGKTLHLERAMGLVADVFTETRLRRLNGNLAIGHVRYSTTGSSQLKNAQPILASCRHG
                     SVALAHNGNLVNAQILKAELETQGSIFNSSTDSEVIVHLIARSKSGDLVESTAEALSR
                     VSGAYSLVIMNETELLGIRDPRGFRPLSLGRLGNAWVLASESCAFDLIEAKFVRDLEP
                     GEFLRITPDGIQSYYPLPATGSAQCIFEYIYFSRPDSLLFGQNV"
     gene            complement(927..2093)
                     /gene="alaC"
                     /locus_tag="AC16G0_17780"
     CDS             complement(927..2093)
                     /gene="alaC"
                     /locus_tag="AC16G0_17780"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HIR5101508.1"
                     /translation="MEDFKRIRRLPPYVFAIVNDMKVKARARGEDIIDFGMGNPDLPT
                     PDHIVDKLCEAVRNPRNHRYSASRGITKLRHAICDWYRRRFGVELDPETEAIATIGAK
                     EGLSHLALAVVESGDVALVPNPTYPIHAYAVIIAGGDVRHVRLGPGEDFFANLLAAFR
                     ESWPQPKLLILSFPHNPTSAVVEPEFFTKIVAFAQEHELIVIHDFAYADLTFDGYQAP
                     SFLQTPGARDVGVEFFTLSKSYNMPGWRVGFAVGNPRIIAALTRIKSYLDYGMFQPIQ
                     IASIIALNGPQDCVGEIVRTYASRRDVLCDGLSRVGWKVDKPRGTMFVWARIPDQFRL
                     KGSLEFSKFLLDQAKVAVSPGIGFGQYGDEYVRFALVENEHRTRQAIQGIKRSL"
     gene            complement(2189..>2561)
                     /locus_tag="AC16G0_17785"
     CDS             complement(2189..>2561)
                     /locus_tag="AC16G0_17785"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIR5101509.1"
                     /translation="ERLSKKVRIQARYRRSLDVLRIAKDLGQPTTKTGIMLGLGETEE
                     EVLTLMRDARAAGCDILTIGQYLQPTKAHLPIVEFVHPDRFKSLEEAGYVLGFRSVYS
                     GPLVRSSYHAELAARRAEGIG"
BASE COUNT          471 a          814 c          801 g          475 t
ORIGIN      
        1 cacgttctgg ccgaacagca ggctgtccgg ccgggaaaag taaatatact cgaagatgca
       61 ctgcgccgag ccggtggccg gcaacgggta ataggactgg atcccgtcgg gcgtgatgcg
      121 gaggaactct ccgggttcca ggtcccggac gaacttggcc tcgatcaagt cgaaggcgca
      181 ggactcggac gccaggaccc aggcattccc caggcgcccc agggacagcg gccggaaccc
      241 gcgaggatct cggatcccca acagctcggt ctcattcatg atcaccagag agtatgcccc
      301 cgacacgcgg ctgagggcct cggccgtgga ttcgaccaga tccccgctct tggagcgggc
      361 gatcaggtgg acgatcacct cgctgtccgt gctggaattg aagatggagc cctgggtctc
      421 cagttcggcc ttcaggatct gggcgttcac caggttcccg ttgtgggcca gggccaccga
      481 gccgtgtcgg cagctagcca ggatcggctg ggcgttcttg agctgcgagc tgcccgtggt
      541 ggaatagcgg acatggccga tggccaggtt gccgttcagt cggcggaggc gggtctcggt
      601 gaagacgtcg gccaccagac ccatggctcg ctccaggtgg agggtcttgc catccgaggt
      661 gacgatcccg gcgctttcct gtccccggtg ctggagcgca tacaacccga ggtaggtcag
      721 gttggccgcc tcgccgctcc ccaggatccc gaagacccca caggcttcat ggaacttatc
      781 cagcatggcc tcccccatca ctcccgaggt agccgatctc cttcagacaa acaaagtgcc
      841 aatgcaaaac cgacaacgga caactgacaa ccggccatcg tggttcattg atcggttgtc
      901 ggttttcaat tttcaattgc cggatctcac aagctccgct tgatcccctg gatcgcctgc
      961 cgcgtccggt gttcgttttc caccagggcg aagcgcacgt actcgtcgcc gtactggccg
     1021 aagccgatcc cgggggagac cgcgaccttg gcctggtcca aaaggaactt ggagaattcc
     1081 agggagccct tcaggcggaa ctgatccggg atcctggccc acacgaacat cgtccctcgc
     1141 ggcttgtcca ccttccagcc gacacgactc aacccgtcgc acaggacgtc gcgacgcgag
     1201 gcatacgtcc ggacgatttc ccccacacag tcctgcggcc cgttcagggc gatgatcgag
     1261 gcgatttgaa tcggctggaa catgccgtaa tccagatagc tcttgatccg cgtcagcgcc
     1321 gcgatgatcc gggggttgcc cacggcaaaa ccgacgcgcc agccgggcat gttgtagctc
     1381 ttggatagcg tgaagaactc cacgccaacg tctctggccc cgggcgtctg cagaaagctc
     1441 ggcgcctggt atccatcgaa ggtcaggtcg gcataggcaa agtcgtggat cacgatcagc
     1501 tcgtgctcct gcgcgaaggc cacgatcttc gtgaagaatt ccggctccac gacggccgag
     1561 gtcgggttat gtgggaagct gaggataagg agcttgggct gaggccagga ttctcgaaag
     1621 gccgccagca ggttggcgaa gaagtcttcc cccggtccca ggcgcacgtg acgcacgtcc
     1681 ccgccagcga tgatcacggc gtaggcgtgg atgggatacg tggggttggg aaccagcgcc
     1741 acatcaccgg actcgacgac ggccagggcg aggtgggata agccctcctt ggccccgatg
     1801 gtggcgatgg cctcggtttc gggatcgagt tccacgccga atcgccggcg gtaccaatcg
     1861 cagatggcgt gacgcagctt ggtgatcccg cgggaggcag agtaccgatg attccgggga
     1921 ttacggacgg cctcgcacag cttgtcaacg atatgatcag gggtgggcag gtccgggttc
     1981 cccattccga agtcgatgat gtcttcgccc cgcgcccgcg ccttcacctt catgtcgttc
     2041 acgatggcga atacgtatgg gggcagcctc cgtatgcgct tgaagtcctc catggggccc
     2101 cctccatccg cgaaaagcgt tcggctgttc gatcgttctg cagttcggct gttccggaac
     2161 ttccgaaccg ccgaacgccc ttggtgtctt atccgatccc ctctgccctt cgtgccgcca
     2221 gttcggcgtg gtacgagctc cgaaccaggg gaccggaata gaccgacctg aaccccagca
     2281 cgtacccggc ctcctccagc gacttgaacc gatcagggtg gacgaattcc acgatcggta
     2341 ggtgcgcctt cgtcggctgg aggtactggc cgatcgtcag gatgtcgcag cccgcggcgc
     2401 gcgcgtcccg catcagcgtc aggacctcct cctcggtctc gcccaacccc aacatgatcc
     2461 cggtcttggt ggtcggctgg cccaggtctt tggcaatgcg caggacgtcc agggagcgtc
     2521 ggtagcgggc ctggatgcgg accttcttcg acagccgctc c
//
LOCUS       DBNGZC010001034         3767 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1750762, whole genome shotgun sequence.
ACCESSION   DBNGZC010001034 DBNGZC010000000
VERSION     DBNGZC010001034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1750762"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="AC16G0_17790"
     CDS             <1..779
                     /locus_tag="AC16G0_17790"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HIR5101510.1"
                     /translation="ADVLDTRQPKRQRRTIALWVWLGGALLLLISGSLYFCFSQNPII
                     VKYRALAQFYSSKREVAVFVSRFGSYAPLAFILIQASQVVLAPVPGEATGILGGYLFG
                     TGLGFLYSTIGLTLGSVLAFGLGRWLGLPIVRRVVTKEVYHKFDFISRAGGELVTLVC
                     FLIPGFPKDYLCFLLGVSPLQFGMFLVISFFGRMPGTWLLSVQGAKVRSAQYTEFVIY
                     LLVAAAAAVLAYIYRDAIFQWMRRRHGDQVGKKSSDTMDL"
     gene            913..2721
                     /locus_tag="AC16G0_17795"
     CDS             913..2721
                     /locus_tag="AC16G0_17795"
                     /inference="COORDINATES: protein motif:HMM:NF025925.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HIR5101511.1"
                     /translation="MAERRATFISRGTRHSTNADATFLELARLVGLQLPSLCGGRGKC
                     AKCRCQPHGPVTEPTALELELLTTEEMARGIRLGCQARPLGVVEVSIEEEVLDKGDYA
                     EVTVEPKPLLRKELLELPIPALHDDLSDIERVQKTLGTQIRVGLEALRTLPLALRQDS
                     FRSTAVLWDHGDAGEELLTIEAGDQREEAYGIAFDVGTTTVVGYLIDLFSWKEVARAS
                     KLNGQQPWGADVLSRSSHALEQDRGLSDLQTAVIGTLNQIIGECCSQIDTRRIYYMTV
                     VGNPIMHHLALGITPATIAVSPYLPVIRDGLSVRAADLGIALPNAWAYFLPLIGAYVG
                     ADTLAVIMATEMDLSDETQLAVDIGTNGEMVLGSRQHLLACSAPAGPAFEGAEIRHGM
                     RATAGAIDRVRIWEDVRVWTIGDVSARGICGSGLIDAVAQMVCRGIVDPRGRLLPRPL
                     QEGRLPSRLLERLAGQGHTWEFVLSQDPYVAVTQDDVRKLQLAKGTIRCGVQILMEEL
                     GIDVSRIARVSLAGAFGNFVDKRSALGIGMLPPAIPQGRILGVGNAAGQGSRMTLLSR
                     TIRQRAEALARRVEYFELSKHPRFEEIFTESMGFTE"
     gene            complement(2889..3089)
                     /locus_tag="AC16G0_17800"
     CDS             complement(2889..3089)
                     /locus_tag="AC16G0_17800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101512.1"
                     /translation="MVKEAEMAEAKTTNLPRLISLLIALVGCVVVAVPIAFNTGGDPA
                     PLILIGSGVTVVALGLFLFFSI"
     gene            complement(3126..3320)
                     /locus_tag="AC16G0_17805"
     CDS             complement(3126..3320)
                     /locus_tag="AC16G0_17805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101513.1"
                     /translation="MSSISIDEAILRELLLRKEELVRSITAGMASGEWDQVMGAFDGL
                     LVAIKRIEESLETEGRKPAS"
     gene            complement(3339..>3767)
                     /locus_tag="AC16G0_17810"
     CDS             complement(3339..>3767)
                     /locus_tag="AC16G0_17810"
                     /inference="COORDINATES: protein motif:HMM:NF014724.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR5101514.1"
                     /translation="LLRVGIRIPDEQENSVKLLEDLRVPRKAILTIQERSDSTRGELQ
                     AVAQFLKEHPARTLIVVTSKAHTRRASKIFSKGLGPGSRLIMHPAGNDPFDPSRWWHD
                     RSDTKSVFYEYLGLVDDWRLWLWGEMLGQVTAVPPAVKVL"
BASE COUNT          657 a         1196 c         1194 g          720 t
ORIGIN      
        1 tggcagacgt cctagataca cgacagccta agcgacagag gagaaccatc gccctatggg
       61 tctggctcgg gggggcattg ctcctcctga tcagcggcag cctgtatttc tgcttctctc
      121 aaaacccgat catcgtcaag taccgggccc tggcgcaatt ctattccagc aagagagagg
      181 tcgcggtatt cgtcagccgc ttcgggtcat acgccccgct cgccttcatc ctcatccagg
      241 cctcgcaggt ggtgctggcg ccggtccccg gggaggccac cggaatcctg ggcggatatc
      301 tcttcgggac tgggttgggc ttcctctact ctacgatcgg cctcaccctg ggatccgttc
      361 tcgcctttgg actcggccgc tggctcggct tgcccatcgt gcgccgggtg gtcaccaagg
      421 aggtgtacca caaatttgac ttcatctccc gcgccggcgg ggagctggtt accctggtct
      481 gttttctcat tcccggcttt cccaaggatt acctgtgctt cctcctgggc gtgagtcctc
      541 tccagttcgg gatgttcctg gtcatcagtt tcttcggccg gatgcccggg acctggctcc
      601 tgtccgtcca gggagccaag gtgcgaagcg cccagtacac ggagttcgtg atttacctcc
      661 tggtggcggc cgcggccgcc gtgctggcct acatctaccg cgacgcaatc ttccagtgga
      721 tgcgccgacg gcacggggac caggtgggca agaaatcgtc ggacaccatg gacctctagc
      781 gcaggttggc cgcaaaaacc tcctcgcgct cgtgtgagca atccccaacg acagtcagct
      841 tcgcggggct gatgattcgc gtgtcagacc ggcacctgcc tgccgaagag cgatatcaga
      901 tgaggagacc ggatggctga gcggagggct acgttcattt cccgaggcac tcgccacagc
      961 accaacgcgg atgccacctt cctagagttg gcgcgcctcg tcgggctcca gctcccgtct
     1021 ctgtgtgggg ggcgggggaa atgcgccaag tgtcgctgcc agccccacgg gcccgtgacg
     1081 gagcccaccg cgctggaact ggagctgctg accacagagg agatggcacg ggggatccgc
     1141 ctgggttgcc aagcccggcc cctgggagtg gtggaggtgt ccatcgagga ggaggtcctc
     1201 gacaaggggg actacgccga ggtgacggtg gagccgaaac cgcttctccg gaaggagctt
     1261 ctggagctgc cgatacctgc gctccacgac gacctatcgg acatcgagcg ggtccaaaag
     1321 acgctgggga cgcagatccg ggtcggcctg gaggcgctgc ggacgttacc gctggcgctg
     1381 cggcaggatt cgttcaggtc cacggcggtc ctgtgggacc acggcgatgc cggggaggag
     1441 ctccttacga tcgaggccgg cgaccagcgg gaggaggcct acggaatcgc cttcgacgtc
     1501 gggaccacca ccgtcgtggg ctacctcatc gatctcttct cctggaaaga ggtcgcccgc
     1561 gcttccaaac tcaacgggca gcagccctgg ggggcggacg tcctttctcg ctcgagccac
     1621 gccctggagc aggatcgcgg gctgtcggac ctccagaccg ccgtcattgg gacgctcaat
     1681 caaatcatcg gcgaatgctg cagccagata gatacacggc ggatctatta catgacggtg
     1741 gttggaaatc ctatcatgca tcatctggct ctgggcatca cgcctgccac catcgccgtg
     1801 agcccgtact tgcccgtgat ccgagacggc ctaagcgtcc gggcggccga cctggggatc
     1861 gccctcccga acgcctgggc gtacttcctg cccctcatcg gagcctacgt gggcgcggac
     1921 accctggcgg tgatcatggc cacggagatg gacctgtcgg atgagactca gctggcagtg
     1981 gatatcggca ccaacgggga gatggtcttg ggctctcggc agcatctcct ggcgtgttcg
     2041 gcgccggcgg gcccggcgtt cgaaggggcg gagatccggc atggtatgcg ggccacggcc
     2101 ggcgccatcg accgtgttcg gatctgggag gatgtccggg tttggacgat cggggatgta
     2161 tcggctcggg ggatctgcgg ctccggcctg atcgatgcgg tggcccagat ggtttgccgt
     2221 gggatcgtgg atccgcgggg acgcttgctg ccccggccgc tccaggaggg ccggcttccg
     2281 tcgaggctcc tggagcgtct ggccggacag gggcacacct gggagttcgt cctgtcccaa
     2341 gatccttacg tggctgtcac ccaggacgat gtgcgcaagc tgcaacttgc caagggcaca
     2401 atccgctgcg gcgtccagat cctgatggag gagctgggga tcgatgtctc tcgaatcgcg
     2461 cgggtgtcgc tggctggtgc attcggcaac ttcgtggaca agcggagcgc gctgggcatt
     2521 ggcatgcttc cccccgctat cccccagggg cgcatcctgg gagtcgggaa cgctgccggt
     2581 caaggatccc gcatgacctt gctttcgaga accatccggc agcgggccga agcgctggcg
     2641 cgccgggtgg agtactttga gctttccaag catccccgct tcgaggaaat tttcacggag
     2701 tctatgggct tcaccgaata gacggcggtc tcccaggggc caggacgtat cctgggctcc
     2761 acaagccacg gagaggaaat cgatgcccgg cgggtgaatg cagggccgga aggcgcagga
     2821 gcttcgctac cggtccgatg cgttcacgtg catgcgagga ggctacccgc ccaggggtct
     2881 gcggccggtc aaatcgaaaa gaagagaaag agaccgagcg caaccacggt gactcccgag
     2941 ccgatgagga tcagcggcgc agggtcccct cctgtattga acgcgatggg aacagccacc
     3001 accacacaac ctaccagggc aatgagaagc gagatcagcc tcggaagatt cgtcgtcttg
     3061 gcctcagcca tttccgcctc cttgaccatt tccgcctcct taaccattcg cgtgtccttg
     3121 cccttctagg atgccggctt ccgtccctct gtctccagac tctcctcaat tcgcttaatg
     3181 gccacgagca ggccatcaaa ggcgcccatc acctggtccc actctcccga cgccatacca
     3241 gccgtaatac tccggaccag ctcctctttc cggaggagaa gctcccgcaa gatcgcctca
     3301 tcgatggaga tgctggacat cgcccgccct tcgtaccgtc agagcacctt caccgctggc
     3361 ggcaccgccg ttacctgccc gagcatctct ccccagagcc atagtcgcca gtcgtccaca
     3421 aggcccagat actcgtaaaa aacgctcttc gtgtccgacc ggtcgtgcca ccagcggctg
     3481 ggatcgaagg ggtcgtttcc cgcggggtgc atgatcagtc gactccccgg ccccagaccc
     3541 tttgaaaaga ttttggaagc ccgccgagtg tgggcttttg aggttacgac gattaacgtg
     3601 cgcgccgggt gctctttcaa gaactgggcc actgcctgca gctccccccg ggtgctgtcg
     3661 cttcgctctt ggatggtgag gattgccttc cggggaacgc ggaggtcttc gaggagcttt
     3721 accgaattct cctgctcatc ggggatccgg atgccaaccc gcaacag
//
LOCUS       DBNGZC010001035         2205 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2731342, whole genome shotgun sequence.
ACCESSION   DBNGZC010001035 DBNGZC010000000
VERSION     DBNGZC010001035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2731342"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="AC16G0_17815"
     CDS             <1..739
                     /locus_tag="AC16G0_17815"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5101515.1"
                     /translation="SYMGAALRLDFGIPFQRPGETVTELIGRTWPVTAHLGLMTIGLA
                     FAVGLPLGIIAAVRQNSWVDYLATVFSLFGFITPHFVIAIGLILLFTNVLGWLPTGGW
                     DGPRQWIMPTLAYAAGPSAIIARFTRSSVVDVIGADYVRTALAKGLSGRAVLLKHVLK
                     NALIPMLTIAGPLAADLITGSFFVETIFRVPGLGKYFTTSIFARDYPMIMACTLLWAT
                     VIAIAFLITDALYAVADPRVKLGEQGR"
     gene            749..1627
                     /locus_tag="AC16G0_17820"
     CDS             749..1627
                     /locus_tag="AC16G0_17820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5101516.1"
                     /translation="MATASRFRMGGAAASLWRDAARRFARNRLAMAGLVGVVGLVLMA
                     TFAPWIAPYHYAKPHYEAVWQFPNPNWPMGTDGLGRDFLSRVIYGSRVSITVGVVVQL
                     VAFVVGVPFGAIAGLHGGKADYLLMRVVDIASAFPRLLFAILLMTLLGSGLSNILIAI
                     AVTSWIEVARLTRGQILSLREKEFVAAAVCLGAGSRRIVMRHLLPNCLTPLIVSLTLG
                     IPRVIFTEAGLSFLGLGVNPPEPSWGQMVGESVAYIRYYWYMALFPAMMIGVTMLAFT
                     LTGDGLRDALDPSRKD"
     gene            complement(1782..>2205)
                     /locus_tag="AC16G0_17825"
     CDS             complement(1782..>2205)
                     /locus_tag="AC16G0_17825"
                     /inference="COORDINATES: protein motif:HMM:NF014609.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIR5101517.1"
                     /translation="SRMKTLIPLKSPEILDALKPDFSRMERLCMSLDCTGLYPFAIHS
                     LPDRIFDARQFPRASGFPEDVATGIAATALAFGLVAYGLIPCDEKRITVQQGRAMGRP
                     SEIFVCFDLPEGGSKPQGCFFGGRAVLSSSRPPSGQTH"
BASE COUNT          349 a          739 c          707 g          410 t
ORIGIN      
        1 gagttacatg ggtgcggcgc tgcgcctcga cttcggcatc ccttttcaaa gaccggggga
       61 gacagtgacg gagctcatcg gccgcacctg gcccgtgacc gcccacctgg gtttgatgac
      121 catcggcctg gcgttcgccg tcggcctgcc gctgggcatc atcgccgccg tcagacagaa
      181 cagttgggtc gattacctgg ccacggtctt ctcgctcttc ggcttcatca cgccccactt
      241 cgtcatcgcc atcggtctca tcctgttgtt caccaacgtc ctgggctggc tgccgaccgg
      301 tggctgggat gggccgagac agtggatcat gccgacgctc gcctatgcgg cgggcccttc
      361 ggcgatcatc gcccgtttca cgcgctccag cgtggtggac gtgatcggcg ccgactacgt
      421 ccgcacggcg ctcgccaagg gtctgagcgg gcgggcggtg ctgctcaagc acgtgctcaa
      481 gaacgccctc atccccatgc tcaccatcgc cgggccgctg gcggcggacc tcatcacggg
      541 gtccttcttc gtcgagacca tcttccgcgt gcccggactg ggcaagtact tcaccaccag
      601 catcttcgcc cgcgattatc cgatgatcat ggcctgcacc ctcctttggg ccacggtcat
      661 cgccatcgcc ttcctgatca ccgacgccct ctacgcagtg gccgacccgc gggtcaaact
      721 gggagagcag ggaaggtagg aaggcgcggt ggccacagcg agtcgcttca ggatgggtgg
      781 ggctgccgcc agcctctggc gggacgcggc gcggcggttt gcccgcaacc gactggccat
      841 ggccggcctt gtcggcgtgg tcggcctggt gctgatggcg accttcgcgc cgtggatagc
      901 gccgtaccat tacgccaagc cccactacga agcggtctgg cagttcccga accccaattg
      961 gcccatgggg acggacgggc tgggccgcga cttcctgagc cgggtcatct acggttcgcg
     1021 cgtgtccatc acggtcggcg tcgtcgtcca actggtggcc ttcgtcgtcg gcgtgccctt
     1081 tggggccatc gccgggctgc acggcggcaa ggcggactat ctactgatgc gggtggttga
     1141 catcgcgtcg gccttccccc gcctgctctt tgccatcctc ctcatgacgc tgctcggcag
     1201 cggcctcagc aatatcctca tcgccatcgc cgtgacgagc tggatcgagg tggcgcgcct
     1261 cacgcgcggc cagatcctgt ccttgcgcga gaaggagttc gtcgccgccg ccgtctgcct
     1321 gggcgcgggc tcacgccgca tcgtcatgcg ccacctcctg cccaactgcc tgacgccgct
     1381 catcgtgtcc ctgaccctgg gcatcccgcg ggtcatcttc accgaggccg gcctgagctt
     1441 cctcggcctg ggcgtgaacc cacccgagcc gagctgggga cagatggtcg gggagagcgt
     1501 ggcctacatc cgctactatt ggtacatggc gctgttcccg gcgatgatga tcggcgttac
     1561 catgctcgcc tttaccctca ccggcgacgg cctgcgcgac gctctcgacc ccagccgcaa
     1621 ggattgaggc tctcgtcggc cgtccgccag gagaggggac ggtcatgaaa ctatgagttt
     1681 ctcaaattgc agatccgagc agctggcgga ttggattccc ggcattcgtg tgatcgcggt
     1741 tggcggttct gaaacgagca gggggagccc cggcgttcgc ctcaatgcgt ttgtccggat
     1801 gggggccgac tgctggacag gaccgcgcgg ccgccgaaga agcagccctg aggcttggac
     1861 ccaccctctg gcaaatcgaa gcacacgaag atctccgatg ggcgacccat cgcacggcct
     1921 tgctgcacgg tgatccgctt ctcatcgcag ggaatcaacc cgtaggcgac aagcccgaag
     1981 gccagggccg tcgcggcgat ccccgtggcc acgtcttccg ggaagccaga ggccctgggg
     2041 aactgacggg cgtcgaagat gcgatcaggc aaggaatgga tcgcaaacgg atacaggccc
     2101 gtgcaatcga gcgacatgca gaggcgctcc atgcgcgaga agtcgggctt gagggcgtca
     2161 aggatctccg gactcttcaa aggaatgagc gttttcatcc gactg
//
LOCUS       DBNGZC010001036         2004 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2634668, whole genome shotgun sequence.
ACCESSION   DBNGZC010001036 DBNGZC010000000
VERSION     DBNGZC010001036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2634668"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="AC16G0_17830"
     CDS             complement(<1..1255)
                     /locus_tag="AC16G0_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003114719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5101518.1"
                     /translation="MNPFELGLDRGPANYTALTPLSFLARSAAVYPEKTAVIHGDRTF
                     TYAEFYARCRRLASALARRGIGVGDTVTIMAPNVPALLEAHYGVPMVGAVLNALNYRL
                     DARSIGFILDHGEAKCLIADTEFSATIEQALALTSRKIPVIAIDDPLNAGQTGRSPLG
                     ETDYEAFLRGGDPDFDWRRPEDEWQAICLLYTSGTTGNPKGVVYSHRGAYLNALGNAL
                     TFGLTPRTVYLWTLPMFHCNGWTYTWAVTAVGATHVCLRKVDPALIFPAIQKHRVTHM
                     CAAPIVLSVLIHAPAEVKARFDHSVEVATGGAAPPSAVVAAMEAMGFRVTHLYGLTES
                     YGPSTVCAWQAEWDALPQKERMAKLARQGVQYPTLEDMMVADSLSLLPVPKDGRTMGE
                     VMLRGNTLMKGYLKNQEATEEAFDGN"
     gene            complement(1269..>2004)
                     /locus_tag="AC16G0_17835"
     CDS             complement(1269..>2004)
                     /locus_tag="AC16G0_17835"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5101519.1"
                     /translation="VLITGASSGLGRHFAHTLARAGAAVALAARSADKLAEAAAEIRS
                     LGGKSVAVTLDVTNGASVREAVATVEAELGPVTILVNNAGVVVGKSLLDHDENDWDRV
                     MDTNLKGAWLVAQEVARRMVAHSQGGAIINIASILGLRVVGGVPVYSASKAGLIHLTK
                     VLALELARHKIRVNAIAPGYITTDLNRDFLQSQAGQAIIRRIPQRRAGEPDDLDGVLL
                     LLASDASRYMTGVVIPVDGGHLTSTL"
BASE COUNT          350 a          643 c          668 g          343 t
ORIGIN      
        1 agttcccatc aaaggcctcc tccgtggcct cttgattctt gaggtagccc ttcatcaggg
       61 tgttgccgcg gagcatcacc tcccccatgg tccggccatc cttgggcacg gggaggaggc
      121 tcagcgagtc cgcgaccatc atgtcttcca gggttgggta ctgcacgccc tgacgagcca
      181 gcttggccat ccgctccttc tgtggaagcg cgtcccattc cgcctgccag gcgcagaccg
      241 tggacggtcc gtaggattcc gtcaggccgt acagatgggt gacgcggaag cccatggcct
      301 ccatggcggc gacgaccgcc gacgggggag cggccccccc ggtcgccacc tccacgctgt
      361 gatcgaagcg tgccttcacc tcggccggcg catggatgag cacggagagc acgatgggcg
      421 cagcgcacat gtgcgtgacc cggtgtttct ggatggcggg gaagatcagg gcggggtcta
      481 ccttccgcag acagacgtgg gtggcgccca cggccgtgac cgcccaggtg taggtccagc
      541 cgttacaatg gaacatgggc agcgtccaca agtagactgt gcgtggcgtc aggccgaagg
      601 tcaaggcgtt tcccagggca ttcaggtagg cgccgcggtg gctgtagacg acccctttgg
      661 gatttcccgt ggtacccgag gtgtagagca ggcagatcgc ctgccactcg tcctcggggc
      721 ggcgccagtc gaagtcggga tcgcctcccc gaaggaaggc ctcgtaatcc gtctctccca
      781 gggggctccg gcccgtctgg ccggcattga ggggatcgtc gatggcgatg acgggaatct
      841 tgcgcgaggt gagggccaac gcctgctcga tggtggcgga gaactccgtg tcggcgatga
      901 gacacttggc ctccccgtga tccaggatga agccgatgga cctggcatcc agccgataat
      961 tgagggcatt gagcacggct cccaccatgg gcacgccgta atgcgcctcg agcagtgccg
     1021 gcacgttggg tgccatgatg gtcacggtgt caccgacccc gatcccacgc cgggcaaggg
     1081 ccgaggccag tctgcggcag cgggcataga actcagcata cgtgaaggtc cgatcgccgt
     1141 gaatgaccgc ggtcttctcc ggatagacgg ccgcggagcg cgcaagaaaa gacaaggggg
     1201 tcagggcggt atagttcgcc ggtccccgat ccaggcccaa ctcgaatgga ttcatttggt
     1261 gtcgcccctc acaaggtgct cgtcaggtgg ccgccgtcca cgggaatgac caccccggtc
     1321 atatagcgcg aggcatcgga tgccagcagc aagaggacgc cgtcgaggtc gtccggctct
     1381 cccgcacggc gctggggaat ccgacggatg atggcctgcc ctgcctggct ctggaggaaa
     1441 tcgcggttga gatccgtcgt gatgtagcca ggggcaatgg cgttcacgcg aatcttgtgg
     1501 cgagccaact ccagcgccag gaccttggtg agatggatga gcccggcctt ggaggcagag
     1561 tacaccggga ctccccccac gacccgaagg ccgagaatcg aggcgatgtt gatgatggcc
     1621 cccccctggg agtgggcgac catgcgtcgg gccacctctt gtgcgacaag ccaggcgccc
     1681 ttgaggttgg tatccatcac ccggtcccaa tcattctcgt cgtggtcgag gagcgacttg
     1741 ccgacgacca cccccgcgtt gttgacgagg atcgtaaccg gccccagttc ggcttcgact
     1801 gtggctaccg cttcgcggac gctggcgcca ttcgtgacgt ccagggtcac agccacggac
     1861 ttgccgccga ggctgcgaat ctccgccgcc gcctcggcca gtttgtccgc cgagcgcgcc
     1921 gccagagcca ccgcagcgcc agcccttgcc agcgtgtgtg caaagtgacg ccccagaccg
     1981 ctcgaggcgc ccgtgatcag aacc
//
LOCUS       DBNGZC010001037         3237 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1360264, whole genome shotgun sequence.
ACCESSION   DBNGZC010001037 DBNGZC010000000
VERSION     DBNGZC010001037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1360264"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="AC16G0_17840"
     CDS             <1..291
                     /locus_tag="AC16G0_17840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5101520.1"
                     /translation="GYVPLNDVTMQIVNGESGAAVDKVMIDGRMVLENGHLLTVDEAK
                     VRVDAERAVARLKEANAGSLAFARSLERYVAAFCIANAKRPTDLRRTVQAEF"
     gene            510..1439
                     /locus_tag="AC16G0_17845"
     CDS             510..1439
                     /locus_tag="AC16G0_17845"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HIR5101521.1"
                     /translation="MASNVAKYIWMNGEYVERSEAKIHVGTAAVLTGANVFEGLRGYY
                     NPDERQVYIFRLQEHLVRLRQSMKVMRMTPPFTSQELTSACTGLVVKNGFREDVQIRP
                     TVYFGEGTPYAYTPDKITTGAFILANPRRCTLDDEKDFACCVSAWRRLSDNMMPPRVK
                     AGANYHQSRLVNMQANVDGYDGAIILNDRDKVAEGPGACVMIVRDGRPIMPPVTAGVL
                     ESITRNTLVDLFAAEMSLQMVEREVDRTELYLADEVFFCGSAWEITPITSVDRYPVGD
                     GKIGPVTRKIRQIYQDVIRGHMPKYKHWLTPAY"
     gene            complement(1718..2458)
                     /locus_tag="AC16G0_17850"
     CDS             complement(1718..2458)
                     /locus_tag="AC16G0_17850"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR5101522.1"
                     /translation="MGQDVLTLSVLGGVTFVATTVQGLTGFAFAVIMMPTVTLLLGIR
                     TAAPLVSLTALTLYIVNSLRYRAWLNFREVWRLGAAAGAGVPVGLWLVAAIADAIVTG
                     FLGAVLIAHGLYGLTHPSPPRRCSSRWAYLAGFLAGSLGGAYNTPGPPLAVYGALRQW
                     PKEEYRAGLQALFFVSGSVTVLSHAVAHHLTSPILTLYAYTAPVLLAGILTGTWLDRY
                     VDTDRFRRLVLAMILLLGLALVLNLGRR"
     gene            complement(2585..>3237)
                     /locus_tag="AC16G0_17855"
     CDS             complement(2585..>3237)
                     /locus_tag="AC16G0_17855"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5101523.1"
                     /translation="TTYYIQQGGTKYSPVRHQKRAQVLCFIRGAGKVQMEKRCFAFSE
                     IAACWEPGSGPVLIQAAGAPVEYLEILIELQALEAVPLHATDPYCVLYSQCEPYAEAI
                     KSPKTVSRTIVPAGTIPRFCMGSVETEGPDTVGAHSHPMLEQLFFGLPGNACVVTADQ
                     AEAALEDRALLHIPSGSWHGTRVEEGRKLHYLWMDFFKHEEDLAYIQAQHQPIKTP"
BASE COUNT          630 a          997 c         1067 g          543 t
ORIGIN      
        1 ggatacgtcc cgctgaacga cgtcacgatg cagatcgtca acggggaaag cggcgccgcc
       61 gtggacaagg tcatgatcga cggccgcatg gtcctggaga atgggcatct cctgacagta
      121 gacgaggcaa aagtccgcgt tgacgccgag cgggctgtcg cccggctgaa ggaagccaac
      181 gccgggagct tggccttcgc gcgctccctc gagcggtatg tggcggcctt ctgcatcgcg
      241 aacgcgaagc ggcccaccga ccttcgccgg accgtgcagg cggaattcta ggtgtcggcc
      301 cgggtcgctt gtcgcacggg ctctcagcgt gctcacttcg gggtcaccga aatccccgag
      361 accagcgttg ctttcgttgt gaggcggctc aaaggcaagg aggcgacggc gatgtctttg
      421 gcttgccgtc cgcttggttt cctgataaaa gccgaccaac agtctgcgga aagtcctggc
      481 ggcaagtgcc cgaaaaggga ggtaatccaa tggccagcaa cgtggcgaag tacatctgga
      541 tgaacggcga gtacgtggag cggtccgagg cgaagattca cgtggggacg gcggctgttc
      601 tcacaggggc gaacgtcttt gaggggctgc gtggttatta caacccggac gagcggcagg
      661 tctacatctt ccgattgcag gagcacctcg tccgcctgcg gcagtccatg aaggtcatgc
      721 gcatgacgcc gcccttcacc agtcaggaac tcacctcagc ctgcacgggc ctggtggtca
      781 agaacggctt ccgcgaggat gtgcagatcc gcccgacggt gtacttcggc gaggggactc
      841 cctacgccta cacgccggac aagatcacca ccggggcgtt catcctggcg aatccccgtc
      901 gctgcacttt ggacgacgag aaagactttg cctgctgcgt cagcgcctgg cggcgcctct
      961 ccgacaacat gatgccgccc cgggtgaagg cgggggccaa ctaccaccag agccgtctgg
     1021 tgaacatgca ggccaacgtg gacggctacg acggggccat catcctcaac gatcgggaca
     1081 aggtcgcgga ggggcctggg gcgtgcgtga tgatcgtgcg cgacggccgg cccatcatgc
     1141 cgcccgtcac cgcgggagtg ctggagagca tcacccggaa cacgctggtc gatctgttcg
     1201 cggccgagat gagcctccag atggtggagc gggaggtgga tcggaccgag ctgtacctgg
     1261 ccgacgaggt attcttctgc ggctcggcct gggagatcac gccgatcacc tccgtggatc
     1321 ggtaccccgt gggggacgga aaaatcggcc ccgtgacgcg gaagatccgc cagatctacc
     1381 aggatgtcat tcgcggccat atgcccaagt acaagcactg gctaacgccg gcgtactgag
     1441 cgtcgccgcg ctcgagcgag cttatcgacc gcaggtggat ctcgggggaa gcggcttcgg
     1501 agtgtgaact ggaggagtca agctacgcta gcgacccgtt ttccactacc gaccaagcat
     1561 ctcatccaag aatcatcgat tgaaggttcg aatccctggt ggggagcaag ttggggctcg
     1621 aaccctacct tgccctgctg cacgtcttgg gaactcgcca gcttggagcc gagataggga
     1681 gttgtcagaa ccgacccttc ggtctacctg ccgagggtca acgtcggccg aggttgagga
     1741 ccagcgctag cccgaggagc aggatcattg ccaagaccag gcgccggaaa cgatccgtgt
     1801 ccacgtagcg atccagccag gtgcccgtca ggatcccggc gaggagaacg ggggccgtgt
     1861 aggcgtagag cgtgaggatg ggtgaggtca aatggtgggc cacggcgtgc gagagaaccg
     1921 tcacgctgcc gctgacgaag aacaaggcct ggaggccggc gcggtattcc tccttgggcc
     1981 actggcgcaa cgcgccatag accgccaggg gcgggcccgg agtgttgtac gcgcccccta
     2041 agctcccggc caggaagcca gccaggtagg cccagcggga cgagcagcgc cggggcgggc
     2101 tgggatgggt gagcccgtac agcccgtgcg caatcaagac agcgcccagg aatccggtga
     2161 cgatggcatc ggcgatggcc gcgaccagcc aaagacccac cggcactccc gcgccggccg
     2221 ccgcccccag ccgccagacc tcccgaaagt tcagccaggc gcgataccgc agactgttta
     2281 cgatgtagag cgtcagggcg gtcagggaga ccaaaggggc cgccgtgcga atgcccagga
     2341 ggagggtgac ggtgggcatc atgatcacgg cgaaggcaaa ccccgtcagt ccctgaacgg
     2401 tcgtcgcaac gaaagtcacc ccgcccagca cactcaatgt gagaacatct tgccccatcc
     2461 ccgtcggctc cccgttttcc acggacctcc ggggcgttct tggccacgcc acggtgagcg
     2521 agtccaaatc ggagatcccc gtcgtgatcg ggtgaaggca gatcagcgtg cggccgcgcg
     2581 gtcctcaggg tgtctttatc ggctgatgct gggcctggat ataggcgagg tcttcctcgt
     2641 gcttgaagaa atccatccac aggtagtgca gtttccggcc ttcctcgacc cgggtgccat
     2701 gccaggaccc tgacggaata tggaggagtg ctcggtcctc caaggctgcc tcggcctgat
     2761 cggcggtcac cacgcacgca ttgccgggca acccaaagaa gagctgttcc agcatgggat
     2821 gcgagtgggc acctaccgtg tcgggtccct ccgtctcgac ggaacccatg cagaaccggg
     2881 ggatcgttcc cgcgggcaca atggtgcggc tgacggtctt cggcgacttg atcgcctcgg
     2941 catacggctc acactgcgag tagagcacac aatatgggtc cgtcgcgtgg agaggcaccg
     3001 cctccagtgc ctgcaactcg attaggatct cgaggtactc caccggtgcg ccggctgcct
     3061 ggatgaggac cggcccgctc cccggttccc agcaagcggc gatctcggaa aaggcgaagc
     3121 acctcttctc catctgcacc ttgcctgcgc cacggataaa gcagaggact tgggcccgtt
     3181 tctggtgacg aaccggcgaa tattttgtcc cgccctgctg gatatagtag gtcgtct
//
LOCUS       DBNGZC010001038         1856 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_676192, whole genome shotgun sequence.
ACCESSION   DBNGZC010001038 DBNGZC010000000
VERSION     DBNGZC010001038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_676192"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="AC16G0_17860"
     CDS             <1..231
                     /locus_tag="AC16G0_17860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101524.1"
                     /translation="IGAGSGALGGTLAGVGAGQATAGTVQSRYDIAYQQCMYAKGNQI
                     PMRGKVQPHNGAPPPPGPSGFPSSPPPPPPPR"
     gene            803..>1856
                     /locus_tag="AC16G0_17865"
     CDS             803..>1856
                     /locus_tag="AC16G0_17865"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5101525.1"
                     /translation="MPSIPFSLLPILFLTLIFYLSFVSRVILGPLLPILEGDLGLGHG
                     EAGSLFLFSASGYCAGLLGSGFVSARLNHRRTIMLSAIAVGASMLVASWSTSVAGLQV
                     GLALLGFSAGLYLPSGIASLTDQVGEAHWGKALSIHEYAPNLGYITAPMLAEALLHFL
                     SWRGVLATVGILGILLGILFQRVGRGGDRWGEPPGFETAKRLLQDSSLWIMAALFAVG
                     IGSTMGIYLMMPLYLVSEIRMDREFANTLIGFSRVAGLAVILFSGLITDRIGHRRALA
                     LFLTATGVLTVLLGILRGPAITPVLVFLQAASAACFFPSAFSIVSHLFVSQVRNLAVS
                     LVVVAGSLVGAGAIPMG"
BASE COUNT          272 a          615 c          579 g          390 t
ORIGIN      
        1 atcggggccg ggagcggggc cctcgggggg acgctggccg gggtcggcgc gggccaagcg
       61 acggccggga cggtgcagtc tcgctacgat atcgcgtatc aacagtgcat gtacgcgaag
      121 gggaaccaga tcccgatgcg cgggaaagtg cagccacata atggtgcgcc acccccgccc
      181 gggccttccg gttttccctc atcgccgccg cctcccccac cacctcgctg agggcaacct
      241 gcccacctgc cccctggcct ccgggtcagg gggttttcgt ttctccgggg acaggggtcg
      301 tcagaaagtt cgccacaaca atgcccctgg aaaggattgc catcgacatc ccggatcgct
      361 gagcgatagg cttccacgcg agccgcccgc caccatctcc acgcgagggg actcactcca
      421 gcccatttca caagaaggtt ctggacccaa ttaaggccaa gtcgtcaaat atgctgaggg
      481 ggcttcggaa ttcttgagat ccatcgtggc agatgtcgtg aagccgtgtc cggtggccga
      541 tgatctgtca gaggcgaccc agccgttctt gcggtgtccc tggcgtcgct tcgaggaccg
      601 gcgcaagatg cctcaagctc gtcgggaata atccagccgg cacaagtctc cgagctgcca
      661 aatgtctgca tccgagtctc ggcgtgagaa aggacttgcc cccagtctgg atcggccgta
      721 gggtttccgg catgagcccg tgccctcgtc tcgcctcccc gtggcgccac aagcctgcgg
      781 ggagtgtctc ggtctgatgc ccatgccctc cattccgttt tccctgctgc ccatcctgtt
      841 tctcacgctg attttctacc tgagcttcgt ctcgcgggtc atccttgggc cgctcctgcc
      901 catcctggaa ggcgacctcg gcctgggtca cggggaggcc ggctcactct tcctgttctc
      961 cgcctcgggg tactgcgcgg gcctcttggg ctcgggattc gtgtccgcac gactcaatca
     1021 ccggcgcacg atcatgttgt ccgccatcgc cgtcggggcg agcatgctcg tcgcatcctg
     1081 gtccacctcc gtggcgggtc ttcaggtggg cctggctctc ctcggcttct cggcaggcct
     1141 gtatctgccg tcgggaatcg ccagcctgac ggaccaggtc ggcgaggcgc actggggaaa
     1201 ggccctgtca attcacgaat acgcccccaa ccttggttac ataacggcgc cgatgctggc
     1261 cgaggcgttg ctgcacttcc tctcctggcg tggcgtcctc gccacggtgg ggattctggg
     1321 gattctcctg gggatccttt ttcaaagagt cgggcgagga ggagaccgct ggggggaacc
     1381 gcctgggttc gagacggcaa agcgtctcct ccaagattcc tccctgtgga tcatggcggc
     1441 gctgttcgcc gtcgggatcg gctccaccat ggggatctac ctgatgatgc cgctgtatct
     1501 ggtgagcgag atccggatgg accgggagtt tgccaatacg ctcatcggat tctcgcgggt
     1561 ggccggcctt gccgtcatcc tgttctcggg actgatcacg gaccggattg gccaccggcg
     1621 ggcactggcc ctattcctga cggccacggg agtcctgacg gtcctgctgg ggatcttgcg
     1681 cggtccggcg atcaccccgg tcctggtctt cctccaggca gcctctgcgg cctgcttctt
     1741 cccctcggcc ttttccatcg tgtctcacct cttcgtatcg caggtccgga acctcgccgt
     1801 atccttggtc gtcgttgccg gctcgctggt cggcgccgga gcgattccga tgggga
//
LOCUS       DBNGZC010001039         4037 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1265790, whole genome shotgun sequence.
ACCESSION   DBNGZC010001039 DBNGZC010000000
VERSION     DBNGZC010001039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1265790"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            438..902
                     /locus_tag="AC16G0_17870"
     CDS             438..902
                     /locus_tag="AC16G0_17870"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5101526.1"
                     /translation="MPNRRSFNRFMCLALVLAVAAPQLAMAAGSGPAPAPVAPRSAES
                     EYNKGLQAKAAKRYSDAIVSFRKAVDIKANYPEAWNELGFALRQTGQYAEAIKAYDEA
                     LRLRPNFPEALEYKGEAYVKMGKLDDAKAILARLTPIDAARAKDLDEAIQGK"
     gene            1240..2436
                     /locus_tag="AC16G0_17875"
     CDS             1240..2436
                     /locus_tag="AC16G0_17875"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008932804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR5101527.1"
                     /translation="MTTTQTFHPPARLLLGPGPSNVEERVLRVMSAQLVGYFDACLLD
                     VAAEISRLLNLAFGTRNKVTFPISGTGSAGMEAALVNVVEPGDKVLVGVSGYFGDRML
                     QIVERCGGVPIALEVEWGKPIQPDQLRAALKKEQGVKVLAVVHAETSTGVLQPLEGLR
                     EIADQQGALFLVDAVTSLAGHPVGVDQHGVDVCYSGTQKALSAPPGLAPVTFSERAME
                     RIRRRTHKVQSFYLDVALFEKYWGTNGSYHHTPPITFYYALLEALKIVEEEGLQARYE
                     RHRLNHLALVAGLEAMGLQMQVEPAYRLWTLNTVKIPNGVDDAKVRGRLLREFGIEIG
                     GGLGTLKGKVWRIGLMGVNSTGNNVLLLLSALERILQSEAFPCSSGIPAANEVYQKNP
                     RSQGAA"
     gene            complement(2900..4003)
                     /locus_tag="AC16G0_17880"
     CDS             complement(2900..4003)
                     /locus_tag="AC16G0_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HIR5101528.1"
                     /translation="MKARAAVLFEARQKLSIEQVDVSDPRAGEVLVHMAAGGVCHSDL
                     HVMKGDLAAPLPAILGHEGAGVVAGVGPEVKDVQPGDHVIPLWRLSCGECEYCTGGRP
                     ALCTAGIEVRRTGTLPGGGTGFTLRGKEIKRFGGVSTFSEYSVIPEKALLKIPKEFPL
                     DRAALLGCAVITGYGAVVNAAQVRPGSLVAVFGAGGVGLNVIQTAALSGAAVVIAVDL
                     RDNKLEFARQFGATHSVNASQGNPVEQVRAITGGRGVDYAFEVVGLPATMRQAFDVLA
                     RRGVAVVVGIGPTTAEVSIPVIPLAWEDRVLMGSLYGSARPRIDILRLIDLYRAGKLK
                     LDELLTRSYPIEQINDAYAALERGEVARSVVTF"
BASE COUNT          768 a         1253 c         1277 g          739 t
ORIGIN      
        1 tataagaatg ctgtgagaga cgacgttagg taagcgtttc ggtcttggtg gcattacccg
       61 cagcagattt ttcgcttcct tgcacgtcaa gccaatccta tcctcggggg ttaaaccatt
      121 cccgccggca gcattgcctc ggcctcttcc ctctcgaacg ggttcctcct cacacctcgg
      181 gcaggcctcc gcgacttcgg ccggataagt atggagaggg gggcggaagg ggggagcgga
      241 ccggtacgcc ttgccgcagc gcacttcgga tacaaccaat gatggacctc ggccgtccgg
      301 ctgcctcaat tcggagaggt acttatggcg catgcttttg agtggaggag gggtaacaaa
      361 atgaaagtgg gaacttttcc caccacttcg gggtttagat agacgaaatc aaccttgaat
      421 cccgaaggag gagccaaatg cccaatcgga ggtctttcaa tcgcttcatg tgtctggcct
      481 tagttttggc cgtggcagcg ccccagttgg ccatggctgc cggcagtggg ccggcaccgg
      541 caccggtggc cccccgttcc gccgaatcgg agtacaacaa gggccttcag gccaaggcgg
      601 cgaagcggta ttcggacgcc attgtcagct tccggaaggc cgtggacatc aaggcgaact
      661 acccggaagc ctggaacgag cttgggtttg ctctgcgtca gaccggccag tacgccgagg
      721 cgatcaaggc ttacgacgag gccctgcgcc ttcggccgaa tttccccgaa gccctggaat
      781 acaagggcga ggcgtatgtg aagatgggaa agctcgatga tgccaaggcg atcttggccc
      841 gcttgacccc catcgacgca gctcgggcga aggacctgga cgaggccatc cagggcaaat
      901 agccaccggg cagcggtgga atgggcagag aacagaccgg acagaagaca gaggtcagag
      961 gacagaaggc agacaacagg aggctgcagg aagaattaac ttgaacgaac ttcgcaggtt
     1021 gggatagagt ttccctggcc tttgtaccag ggatcccatc cctcatgcgg gtggacgccc
     1081 gcagggttcc tgggttcggc gtgggtttgg atcgaggtgg ctcgcctggg cgcagaggtt
     1141 gcgccctcgc cccgattccc ctcgctacaa cgggaagcca cggaggcaca tgagaaaagg
     1201 cccccgtggc ttttttgttt cccagaggag gaaggaccca tgacaaccac gcaaaccttt
     1261 catccacccg cgcgactcct gctcggcccc ggccccagca acgtggagga gcgggtgctc
     1321 cgcgtcatga gcgcccagct cgtcgggtac tttgacgcct gtcttctgga tgtggcggcg
     1381 gagatcagcc gtctcctcaa tctcgccttc ggcacgagga acaaggtcac cttccctatc
     1441 tccggcaccg gcagcgctgg gatggaagcc gccctcgtca acgtcgtcga gcctggagac
     1501 aaggtcctgg tcggtgtgag tggctatttc ggcgaccgga tgcttcagat cgtcgagcga
     1561 tgtggagggg tgccgattgc cctggaggtg gagtggggga agccgattca gcccgaccag
     1621 ctccgcgccg ccctcaagaa ggagcagggc gtgaaggtcc tcgccgtggt gcatgcggag
     1681 acctcgaccg gcgtcctcca accgctggag gggttgcgag agattgcgga ccagcagggc
     1741 gccctcttcc tggtggatgc ggtgaccagc ctggcaggtc atcccgtggg cgtggatcag
     1801 catggggtcg atgtgtgcta ttcaggtacc cagaaggccc tgagcgcccc accgggcctc
     1861 gcaccggtga cgttctccga gcgggcgatg gaacggatcc ggcgacgaac ccacaaggtc
     1921 cagagcttct atctggacgt cgccctcttc gagaagtact gggggacgaa cggttcttac
     1981 caccacactc cgcccatcac gttctactac gccctgctgg aggccttgaa gatcgtcgaa
     2041 gaggaagggc ttcaagctcg ctacgagcgt caccgcttga accacttggc attggtggcg
     2101 gggttggagg ccatggggct ccagatgcag gtcgagcccg cgtaccgtct ctggaccctg
     2161 aacacggtga agattccgaa cggcgttgac gatgccaagg tccggggccg cctcctccgg
     2221 gagttcggga tcgagatcgg tggtggcctg gggacgctca aggggaaggt ctggcgaatc
     2281 ggcctgatgg gagtgaactc gacgggaaat aacgtcctct tgctcctctc ggccttggag
     2341 cggatcctgc agtccgaagc ttttccgtgc agctcaggca tccccgcagc caacgaggtc
     2401 taccagaaga atccgcggag tcaaggcgcg gcataagggg aactgccaag tgtctgccgg
     2461 tgcgtgagcg ttgcatccaa gggacaaacg gtgcctggcg gaatgtccgt gccggggtgc
     2521 tcgctgaagg tgtggcggag agtgatacgg cccttcggag aggatgaatt ctccggaggg
     2581 ccgttgtgtt tccggacggg ttgtgtgtag accggccagc attggcctac ataccctagg
     2641 ccttcacgag aagggcaaat tctccgtgcc tgccggtcaa cggctagctc tgatccgaaa
     2701 ttcggcatct cgccaagatc acccaacccc aggctgggta cccggatcgc caagacccac
     2761 gatcataatc tgggcacaaa aggcaagaga cccaccgccc ctggagggac tcatccgata
     2821 tgggtgattc cgtgattaaa tctggccttt ctttgcgccc ttggcgtctt tgcgagagac
     2881 ggatttcttc ggattggggc tagaaggtca ccacgcttcg ggcaacttca ccgcgctcca
     2941 gcgcggcgta ggcgtcgttg atctgctcga tgggatagct gcgcgtcagc aactcgtcca
     3001 attttagctt gccggcccgg tacaggtcga tgaggcgcag gatgtctatg cggggccgcg
     3061 ccgagccgta gagtgatccc atgaggaccc ggtcctccca agccagcggg atcaccggaa
     3121 tggagacctc ggcggttgtc gggccgatgc ccacgaccac cgccacacct cgtctggcca
     3181 agacatcgaa cgcctgccgc atggtggcgg gcaggcccac cacctcaaag gcgtagtcca
     3241 cacctcggcc cccggtgatc gcccggacct gctccacggg attgccctgg gatgcattca
     3301 cgctgtgggt ggcgccgaac tgccgcgcga actccagctt gttgtcccgg agatccaccg
     3361 cgatgaccac cgcagccccg gacagggccg cggtctgaat cacattgagg ccaaccccgc
     3421 ccgcaccaaa gacggccacc aggctgccgg gccggacctg ggccgcgttg accacggctc
     3481 cgtaaccggt gatcacagcg catccgagca gggcggccct gtccagggga aactccttcg
     3541 ggatcttgag cagcgccttc tccggaatca ccgagtactc cgagaaggtg gagaccccgc
     3601 cgaatcgctt gatctccttc cccctcagcg tgaaaccggt cccgccgccg gggagcgttc
     3661 cagtcctccg gacctcgatg ccggccgtgc acagcgcagg gcggccgccg gtgcagtact
     3721 cgcactcgcc gcaggagagc cgccagagcg ggatcacgtg gtctcccggc tgcacgtcct
     3781 tcacctcggg tcccaccccg gcgacgaccc cagctccttc atgtcccaga atcgcgggca
     3841 atggcgcagc caggtcgcct ttcattacgt gcaggtcact gtggcagacc ccgcctgccg
     3901 ccatgtggac cagcacttcc cctgcccgcg gatccgatac gtccacctgc tcgatcgaca
     3961 gcttctgccg cgcctcgaac aacaccgctg ctcttgcctt catggttcgg ctccctgcgc
     4021 tgagtgtgtt tggaaac
//
LOCUS       DBNGZC010001040         3457 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_774038, whole genome shotgun sequence.
ACCESSION   DBNGZC010001040 DBNGZC010000000
VERSION     DBNGZC010001040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_774038"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="AC16G0_17885"
     CDS             <1..872
                     /locus_tag="AC16G0_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5101529.1"
                     /translation="TGEPSELDLLAMACLLPSLLYVLVLFVYPFLYGIYQSVHPLKVA
                     GFSLANYVAFFTDEFQYKTVWITFRLAVPNTIVVVAISLLFAYNMRRGIWLERTITTI
                     LVLPISLGVILLGQGILGFYGGKGWFNQLLLGLGVLEDGLTLTHNFTGVMLSLFMQQF
                     PFCFLMRLGYISGIDPSIENSARTLGANPWTVFRRVMLPLIAPGLAIAFALVFVMSFG
                     TFASAIMVGQPSGSTRTIAIAAYAQAFEMFDMPYASAIAVIMGLFQLAGLLVILLLRK
                     RMVTAATMGVGKR"
     gene            964..1785
                     /locus_tag="AC16G0_17890"
     CDS             964..1785
                     /locus_tag="AC16G0_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012978361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5101530.1"
                     /translation="MRGRMWGLAVYMFVLGFILNLLGIVSHVVVSSFAKRWFGTPFPR
                     ALTTEWYSYAWKEFGLAQVLGVTVLVALSVVFLALLMGFPAAYILARRNFQWKPVLLL
                     LYFLPLLIPQMTYGIPLATTMFRYGIGGSVPGVILAVMVPMVPLAIFILMPFFEQISI
                     NLEWGAAMMGANRFQIFRRVLLPLTLPGLLTAGILVLVNTVSNFELTFLVSGAGSQTL
                     VVALFYNVFAGGVRPVYSVDAMAVMYMTMVMLLLIIALRFVRPTQMVFRLDQPRK"
     gene            complement(1906..2286)
                     /locus_tag="AC16G0_17895"
     CDS             complement(1906..2286)
                     /locus_tag="AC16G0_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HIR5101531.1"
                     /translation="MGYRLLADLLVLVHLAFVLFVVLGGLLVLRWPRLAWLHIPAAAW
                     GAFIEAVGWICPLTPLENLLRRHAGEAGYAGGFIEHYIITLLDPPGLTRNVQFVLTAI
                     VVAVNLAVYAVLFRRWGSARKSRP"
     gene            complement(2472..2975)
                     /locus_tag="AC16G0_17900"
     CDS             complement(2472..2975)
                     /locus_tag="AC16G0_17900"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HIR5101532.1"
                     /translation="MEQTGAYWYALRTRSRHEKRVEEQLSAQGIEPFLPLIQRWRQWK
                     DRRKQVAFPLFPGYCFARFLPKDRVTVLKSHGVVQIIGNREGPVSVPEAEIEAVRRLV
                     TSTLPYDPHPYLHEGMPVEVIRGPLAGVRGVLLRKGARARLVISVSLIQQAASVELDA
                     TDVAPLS"
     gene            complement(3125..>3457)
                     /locus_tag="AC16G0_17905"
     CDS             complement(3125..>3457)
                     /locus_tag="AC16G0_17905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101533.1"
                     /translation="ILTAWLIGVSAAGTGWAQPAVSEAPASSTDLSGTQFLAALAGGV
                     GSLFYIPFKALAICPGMALASGASLAVTGGNRDTAEYLLRVGCTGTYFITPGMVRGQE
                     EFRGSGDP"
BASE COUNT          585 a         1136 c         1051 g          685 t
ORIGIN      
        1 gcaccggcga accgagcgag ctggacctgc tggcgatggc ctgcctgctc ccctcgctcc
       61 tttacgtgct cgtgctgttc gtctacccgt ttctctacgg aatctaccag agcgtgcatc
      121 cgctcaaggt ggccggcttt agcctggcca actacgtggc cttcttcacc gatgaatttc
      181 agtacaagac cgtgtggatc accttccggc tggccgtccc caacaccatt gtggtggtag
      241 ccatcagcct gcttttcgcc tacaacatgc ggcggggaat ctggctggag cgaaccatca
      301 ccacgatcct ggtcctcccc atctcgctcg gcgtgatcct gctgggccag gggatcctgg
      361 gattctatgg ggggaagggg tggttcaacc agttgctcct gggcctgggc gtgctggagg
      421 atgggttgac gctcacccac aacttcaccg gagtgatgct ctccctgttc atgcagcaat
      481 ttcccttctg cttcctcatg cgtctgggtt acatctcggg catcgacccc agcatcgaga
      541 actcggcgcg cacactcggg gcgaacccct ggactgtctt ccggcgggtc atgttgcccc
      601 tgatcgcccc cggactcgcc atcgcgtttg ccttggtctt tgtgatgagc ttcggtacct
      661 tcgcctcggc catcatggtg gggcaaccat cgggctccac tcggaccatc gccatcgccg
      721 cctatgcgca ggccttcgag atgtttgaca tgccctacgc ctcggccatc gcggtgatca
      781 tgggactgtt ccagttggcc ggcctgctgg tgatactgct cctgcgcaag cgcatggtca
      841 cggcggctac catgggggtc ggcaagcggt aaagacgcca acgaataccg ggaaacggac
      901 acccggtcaa tggccctttg atttcaggaa tcgtttgtcc ttgaggaggt ctgaggaaaa
      961 gagatgcgcg gccgaatgtg gggactggca gtgtacatgt tcgtgctggg gttcatcctg
     1021 aacctgcttg gcatcgtgag ccatgtcgtg gtcagctcgt tcgccaagcg gtggttcggc
     1081 accccgtttc cgcgcgcgct caccaccgag tggtacagct atgcctggaa ggagtttggc
     1141 ctcgcccagg tcctgggggt gaccgtcttg gtagccctga gcgtggtttt cctggccctt
     1201 ctcatggggt tcccggccgc ctacatcctg gctcgccgca acttccagtg gaagccggtg
     1261 ctgctcctgc tctacttcct ccccctcttg atcccccaga tgacctacgg catcccgctg
     1321 gcaaccacca tgttccggta cgggatcggc gggtccgtgc ccggcgtcat cctggccgtg
     1381 atggtcccca tggtccccct ggccatcttc atcctgatgc ccttcttcga gcagatcagc
     1441 atcaacctcg agtggggcgc cgccatgatg ggggccaacc ggttccagat cttccgccgc
     1501 gttctcttac cgctcaccct gcccgggctc ctcacggccg ggatcctcgt cctcgtgaac
     1561 acggtctcga acttcgaatt gaccttcctg gtctcggggg ccgggtccca gacgcttgtc
     1621 gtggccctgt tctacaacgt cttcgcgggt ggggtccgcc ccgtctattc ggtggacgcc
     1681 atggccgtga tgtacatgac catggttatg ctcctcctca tcatcgccct gcgcttcgtc
     1741 cgtccgacgc agatggtctt ccgcctcgat cagccgcgga agtaaactgc gcgcctggct
     1801 tcacctatcg gaagcgtccc cgcatccgtt ccgaacggaa aatcacgaaa gtaccattgt
     1861 tctcgtcgcc agttcttttc aggcgtgtgc gcgaatcagc gggaattacg gcctggactt
     1921 tctggccgag ccccagcgtc ggaagaggac cgcgtagact gcgaggttga cggccacaac
     1981 gatggcggtc aggacgaact ggacgttgcg ggtgagcccc ggcgggtcca ggagtgtgat
     2041 gatgtagtgt tcgatgaagc cgccggcata ccccgcctca cccgcatgcc gccggaggag
     2101 attctccaga ggggtcaggg ggcaaatcca gcccacggct tcgatgaagg cgccccaggc
     2161 ggcagccggg atgtggagcc aggcgagtcg gggccatcgc agaaccagga gccccccgag
     2221 gacgacgaag aggacgaacg ccaggtggac caggaccaag aggtcggcga ggagccggta
     2281 acccatgcgt gcccctccac ccccgatctc acaccctacc catccactcg gtaccgcgtc
     2341 acgacctggg ggttgacctc cattcccaga ccggggcgat cagtaggctc gatgtggccg
     2401 gtgtgatcga cgaccatggg ccccttcatc agctccgcgc cttctgcatc ctgcgagcgg
     2461 acgttcgagg ctcaggacaa gggggcaacg tccgtcgcgt ccagctccac ggatgcagcc
     2521 tgctgaatca ggctgacgct gattacaagt ctcgccctcg cccccttgcg gagcagcacg
     2581 ccgcggaccc cggccagggg gccgcggatc acttcgaccg gcatcccctc gtggaggtag
     2641 gggtgggggt cgtagggcag cgtactggtg acgagacgac gcacagcctc gatctcggcc
     2701 tcggggaccg agacgggccc ctcgcgattg ccgatgatct gcacgacgcc gtgggacttc
     2761 aggacggtga ctcgatcctt ggggagaaag cgtgcgaagc aatagccagg aaacagcgga
     2821 aaggccacct gcttgcgacg gtctttccac tgccgccacc gctggatcag cggcaggaag
     2881 ggctcaattc cctgggccga gagttgttcc tccacgcgct tttcgtggcg cgagcgagtg
     2941 cgaagggcat accagtaggc accggtctgt tccatggctt cattcgatcc tctgcagggg
     3001 ggccccatgc tgcatgagtc ctgcggggaa gactatccga aacctgaaac ggcaagacgt
     3061 atacgcattg cagcgagcgg aacccgccgt cagcgtggcc cgtcgtcagc attgacacct
     3121 cagcctatgg gtccccggat cctcggaact cctcctgacc ccgaaccatt ccaggggtga
     3181 tgaagtacgt ccccgtgcag cctacgcgca acaagtactc tgccgtatca cggtttcccc
     3241 cggtcaccgc caggctggcc cctgacgcca gcgccattcc ggggcatatc gcgagggcct
     3301 tgaatgggat gtagaagagg ctgcccacac cacccgccag ggcggccaga aattgcgtcc
     3361 cggagagatc cgtgctggat gccggcgctt ccgaaaccgc cggctgggcc caccccgtcc
     3421 ctgcggcgct caccccgatc agccaggccg tgaggat
//
LOCUS       DBNGZC010001041         2174 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2438169, whole genome shotgun sequence.
ACCESSION   DBNGZC010001041 DBNGZC010000000
VERSION     DBNGZC010001041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2438169"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="AC16G0_17910"
     CDS             <1..707
                     /locus_tag="AC16G0_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101534.1"
                     /translation="VALRVAPMFLAGRNLTRMGGVILLGMNGAVILGLLAEGWPPASR
                     PAQLLQAFAELGVSAALIAAAVAVGAWRHEPRHTLALHLDRTEARFFRLAFASAGLAA
                     AGLTLGAVLAMTGTPRGLLVDAVRHLLTVGFVIGMICAMGFRFLPVIEGVALPLPWLR
                     AVCFWALATSTILRTLEVGADYLHEGFLRPAAVSGFLAWLALGCWGLAASLTMLGGIR
                     ARRAGRAVPSLPSSPA"
     gene            792..1502
                     /locus_tag="AC16G0_17915"
     CDS             792..1502
                     /locus_tag="AC16G0_17915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101535.1"
                     /translation="MLFGLVQVVKKAPAGVRLAIRARPKVFITVTIGVFLLDLFLPPL
                     VLSLARKPVDFFTFNPWLPKLPGYLVSNQAPAATRFGFLLRFALFWFSADSPYGGVDW
                     GYAVDASDLIRFAFTSLVVGAYFALWLYRRDQRQHVGLTGRMGRHGGALGAIASVLGF
                     STGPCSVMGCGAPVIPVLGLAFIGLSSGTLTLLTELSKVGTTIILAALTLAVLYLGWL
                     VSADFRLYQSTRYGHVNT"
     gene            1786..2067
                     /locus_tag="AC16G0_17920"
     CDS             1786..2067
                     /locus_tag="AC16G0_17920"
                     /inference="COORDINATES: protein motif:HMM:NF015862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG/HbpS family heme-binding protein"
                     /protein_id="HIR5101536.1"
                     /translation="MRELLVSLMLCGIVLFGAQAQAQQQAPPAQVPEKMPFDIPYGTT
                     ITLERAKPLIAAAQAEAKKHDWKLAIAVVDPAGDLVAFEKMDGTQLASV"
BASE COUNT          318 a          743 c          663 g          450 t
ORIGIN      
        1 gcgtcgctct gcgcgtggcc cccatgtttc ttgccggccg caacctcacc cgaatgggtg
       61 gagtgatcct cctcggcatg aatggcgccg tgatcctcgg cttgctggcc gagggttggc
      121 ccccggcgtc gcgtccggct caactcctcc aggccttcgc agaactgggg gtctccgccg
      181 ctctcatcgc tgccgccgtg gccgtgggcg cctggcgaca cgagccccgc cacacgcttg
      241 cccttcacct ggatcggacc gaagcgagat ttttccgttt ggctttcgcc agcgccggcc
      301 ttgcggcggc cggtctcacc ctgggggctg tgctcgccat gacgggcacc ccgcgcggcc
      361 tcctggtgga cgccgtgcgc cacctgctca ccgtggggtt cgtcatcggc atgatctgcg
      421 caatgggatt tcgattcttg cccgtgatcg aaggcgtcgc gctgcccctc ccctggctgc
      481 gcgccgtctg cttttgggcc ctggcgacca gcacgatcct ccgcacgctg gaggtcggag
      541 cggattacct gcacgagggc tttctccgcc ccgctgccgt ctcggggttc ctcgcatggc
      601 tcgcgctcgg ctgctggggg ctggccgcga gtctcaccat gctcggaggg atccgcgcac
      661 gccgtgccgg acgtgcggtc ccgtctctgc cgtcttcccc cgcttgatct tcagagaacc
      721 gtcacaggac gaaaagggct tgcccggccc cggttggcat tgtaataaat gatcatccga
      781 ggggtgaggg gatgcttttc ggactcgtcc aagtagtcaa gaaggcaccc gctggggtca
      841 ggctggccat ccgggctcga ccgaaggtct tcatcacagt cacgatcggc gtcttcctcc
      901 ttgacttgtt cctgcctcct ctggttctct ctctggctcg gaagcccgtg gacttcttca
      961 cgttcaatcc gtggctgccg aaattgcccg ggtacctcgt ctcaaatcag gcgcccgccg
     1021 ccacgcggtt cgggttcctt ctcaggtttg cccttttctg gttctcggcg gacagcccgt
     1081 acgggggtgt ggattggggg tacgctgtgg atgcgagcga cctcatcagg ttcgcattca
     1141 cctcactcgt ggtcggcgcc tacttcgccc tctggctgta tcgccgagac cagcgtcaac
     1201 acgttggatt gactggaagg atgggccgac acgggggggc gctgggcgct attgcgagcg
     1261 tgctcgggtt ttccacaggg ccttgcagcg tgatggggtg cggcgccccc gtcatcccgg
     1321 tcctgggatt ggccttcatc ggcctctcca gcggcacgct cacgcttctg accgaactct
     1381 ccaaggttgg caccaccatc atcctcgccg ccttgacact ggccgtgctg tacctcggat
     1441 ggctcgtcag cgccgacttc cgactctacc agtccacccg gtacggacac gtcaacacct
     1501 gagattcatc tttactggtc gacaaccaag cccctgcgga cccgcgtgag gcatcttcag
     1561 gcgactttcc gtatctggat cgcgtttcgc cctcccggct ggctatagcc ggcccggatc
     1621 gcgtagcgaa cgtcatggca ctggattcaa tctcgcctga cacacgggtc agccctgcgt
     1681 tgtctacgag caggtccggc tgaaaaggcg ctacctggac tgccccgccg ggagaggcgg
     1741 gcacccgctt ttcaccttgt caaacgaaac agagggagga tgcacatgcg tgagctgctt
     1801 gtgtcattga tgctttgcgg tatcgttctg ttcggcgcgc aggcgcaggc gcagcagcag
     1861 gcgcccccgg cccaggtccc cgagaagatg ccgttcgaca tcccctacgg caccacgatc
     1921 accctcgagc gcgccaagcc gcttatcgcc gcggcccagg ccgaggcgaa gaaacacgat
     1981 tggaagctgg ccattgcggt cgtggacccg gccggggacc tcgtcgcctt cgagaagatg
     2041 gacggcactc agctcgcctc ggtgtgaatc tcgcagggca aggcccttac agccgcacgc
     2101 ttccgccgtg agacccgggt gttcttcaat gcgatggaaa ccggccatcc ctatatcgac
     2161 actctcgacc ccac
//
LOCUS       DBNGZC010001042         1706 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_676669, whole genome shotgun sequence.
ACCESSION   DBNGZC010001042 DBNGZC010000000
VERSION     DBNGZC010001042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_676669"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="AC16G0_17925"
     CDS             complement(<1..559)
                     /locus_tag="AC16G0_17925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101537.1"
                     /translation="MLIPMLVMGCAHTEQTQPTEGRVFFRAGTAATGTSAAAVASGPS
                     VRRLGMLTPTFDPPWQATWIEGEPKAMGGQGAATGFVTGMAILQAMPIVILTWPAAVG
                     VVVGATALGALGQHIENTPLAHVSSEDRATLLAAAASLHPDRLLRDAMANMLTDLTAR
                     PPIPILWYPTWGPDTQGTDPLADART"
     gene            complement(841..1353)
                     /locus_tag="AC16G0_17930"
     CDS             complement(841..1353)
                     /locus_tag="AC16G0_17930"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013705942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR5101538.1"
                     /translation="MTQHPSDFSFCPRCGGALELRTLKAGEPQRLVCQACGFIFFQDP
                     KVATGAIFSLNGGIILVQRAIEPRSGKWVFPGGYVDRGETLEAAALREVKEESGLEVR
                     LTRLLGAYSSPGNAVILIVYAGEVTGGTLRTDDENQDIRPFPPAEIPWDELAFPSTRQ
                     ALRDYLGFAG"
     gene            complement(1350..>1706)
                     /locus_tag="AC16G0_17935"
     CDS             complement(1350..>1706)
                     /locus_tag="AC16G0_17935"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR5101539.1"
                     /translation="ADDQASADRPASPLVLINPRIAPSSDQMEEGWEGCLSLPNLRGK
                     VPRYKEIEVEAVDRRGRRIHRKAADFYARVIQHECDHLAATLFIDRMKDFQTLTFLDE
                     YARYWAPREESEDVEA"
BASE COUNT          294 a          546 c          601 g          265 t
ORIGIN      
        1 gggtgcgtgc atccgccaga ggatcagtgc cttgagtatc aggcccccag gtgggatacc
       61 acaggatagg gatcggcggt cgggccgtca gatcggtcag catattcgcc atggcgtcgc
      121 gcagcaggcg gtcaggatgc aaactggcgg ccgcggccag gagggtggcg cgatcctcgc
      181 tgctcacgtg cgccaggggc gtattttcaa tatgctggcc gagagcgccc agggcggttg
      241 cccccacgac caccccgacg gcggcaggcc acgtcaggat gacgatgggc atcgcctgga
      301 ggatcgccat cccggtgacg aatccggtgg ccgcaccttg gccccccatc gccttcggct
      361 ctccttcgat ccaggtcgct tgccatgggg gatcaaaggt gggtgtgagc ataccgaggc
      421 gccggacaga cggaccgctc gcgacggcgg cagcgctcgt tcccgtggcg gcggtccccg
      481 cacggaagaa aacccgaccc tcggtcggtt gcgtctgttc ggtgtgtgcg caccccatga
      541 cgagcatggg gatgagcatc gaggcgagga tggcccgaat gcgccgccct ttcccgcctc
      601 cgacaggcag cccctcgtcg ggcggcaggg gctggtcagg acggccccgc aggagaaggc
      661 cagggggagg gtctggagga gcgaagccgg gtgacctcat ccgcggaaca atagcaccga
      721 gccgggatgc ggacaatgaa gatttcgacc gcacgccggc cccagccatc ggcggtcagc
      781 gtctcggaga gtgtcgagga ctggggtgaa cagggcgtca ggtgggtcca ggccccggaa
      841 tcagcctgca aaacccaggt agtcgcgcag ggcctgcctg gtgctaggaa aggcaagctc
      901 atcccagggg atctcggccg gagggaaggg ccggatgtcc tggttctcgt cgtcggtccg
      961 gagagtgccg cccgtcacct cacccgcgta cacgatgagg atcaccgcat tgccggggga
     1021 agagtaggcg ccaagcaaac gggtcagtcg gacctccagg ccggactcct ccttgacctc
     1081 gcggagggcg gcggcctcca gggtttcccc acgatccaca tacccgccgg gaaagaccca
     1141 cttgccggag cgcggctcga tggcgcgctg gacgaggatg atcccgccgt tcagggagaa
     1201 gatggccccg gtggccacct tggggtcctg gaagaatatg aagccgcacg cctggcacac
     1261 gagccgctgt ggttcgccgg ccttgagcgt gcgaagctcc agggcgcccc cgcagcgtgg
     1321 gcagaaggag aaatcggagg ggtgctgggt cacgcctcga cgtcctcgct ctcctcccga
     1381 ggcgcccaat agcgtgcata ctcgtccagg aaggtcagcg tctggaagtc tttcatccgg
     1441 tcgatgaaga gcgtggccgc caggtggtcg cactcgtgct ggatcacccg ggcgtagaaa
     1501 tcagcagcct tgcggtggat ccgcctgccc cgccggtcca ccgcctcgac ctcgatctcc
     1561 ttgtagcgcg gcaccttccc ccggagattg ggcaagctca ggcagccttc ccagccctcc
     1621 tccatctgat cgctggaggg tgcgatccgt ggattgatca ggaccaaggg ggaggcgggc
     1681 cggtctgcgg aggcctggtc gtccgc
//
LOCUS       DBNGZC010001043         7731 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1064676, whole genome shotgun sequence.
ACCESSION   DBNGZC010001043 DBNGZC010000000
VERSION     DBNGZC010001043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7731)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7731)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7731
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1064676"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..1374
                     /locus_tag="AC16G0_17940"
     CDS             196..1374
                     /locus_tag="AC16G0_17940"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014866372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010285 -
                     L,L-diaminopimelate:2-oxoglutarate transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HIR5101540.1"
                     /translation="MGGHVQARRLQVLPPYVFAELDRMKQEQVKRGVDIISLGIGDPD
                     LPTPPHIIQALAMAAADPRNHQYPSYEGMLSFRKAVADWYQARFGVALDPNKEVLTLI
                     GSKEGVGHLPLALIDPGDVVLVPDPAYPVYQAGTLFAGGEAFPLPLTRERGFLPDLDA
                     VPADVLGRARILWLNYPNNPTGAIAPREFLAKAVEFARRHRLILAHDAPYSELAFDGY
                     RPESILSIEGAKEVAIEFHSVSKTYNMTGWRLAFAVGNAEILAGLGRIKQNLDSGVFQ
                     AVQFAGIAALTGPQQCVAENCRIYQERRDVLVKGFREMGFEVASPKATFYLWLPVPKG
                     FTSASFTAELLVKAGVVVTPGSGFGAAGEGYVRSTFTVSVDRIREAVERIKKLGIRGG
                     "
     gene            1431..1787
                     /gene="folB"
                     /locus_tag="AC16G0_17945"
     CDS             1431..1787
                     /gene="folB"
                     /locus_tag="AC16G0_17945"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR5101541.1"
                     /translation="MQDRILIQGIQFYGYHGVRAEERKLGQLFQADVELRLDLRAAGQ
                     RDDLAASVDYGKVSAAVMDVGTHDPCKLLERLAERIATVLLERFSVEQVTVRVTKPHP
                     PMSNVIGGVSVEITRP"
     gene            1784..2284
                     /gene="folK"
                     /locus_tag="AC16G0_17950"
     CDS             1784..2284
                     /gene="folK"
                     /locus_tag="AC16G0_17950"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008633520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HIR5101542.1"
                     /translation="MSVQAYIAIGSNLGDRRANCHQALDRLGRLPGTTLLRVSSLIET
                     QPEEGVAGGAFLNGVAEIVTELAPCPLLNHLQAIEVSLGRVAPHGRGTARTMDLDLLL
                     YGDLVMHEADLSIPHPRMASRRFVLAPLAELAPGLHHPVLRVMIQELLRRLDPTGCVS
                     PSGVPA"
     gene            2281..2937
                     /locus_tag="AC16G0_17955"
     CDS             2281..2937
                     /locus_tag="AC16G0_17955"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HIR5101543.1"
                     /translation="MKGTPTSPRYIVVEGPIGVGKTSLVERLSHRLGARTLLEVVEDN
                     PFLGEFYKDPHRYAFQTQLWFLLNRYKQQQELAQFDLFGQTLVTDYLFAKDKIFAYVN
                     LEDHELALYERIHALLEVRVPTPDLVIFLQASTEALLSRIGTRGKPYERGITAKYLED
                     LNAAYTHFFFHYTASPLLVVITSDIDFVNRPEDLEDLVKEIGQTGGGTHYYVPLGSRR
                     "
     gene            3153..3998
                     /gene="panC"
                     /locus_tag="AC16G0_17960"
     CDS             3153..3998
                     /gene="panC"
                     /locus_tag="AC16G0_17960"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HIR5101544.1"
                     /translation="MQIIQGPAEMQRMVEESRQAGKSMGLVPTMGALHDGHLSLIRRC
                     RAENDVVLMSLFVNPTQFNRKDDLDRYPRDLDRDARLARSAGVDVIFAPAVEGIYPEG
                     FATYVSVERLTDRWEGAARPGHFRGVATVCAKLFTICRPHRAYFGQKDYQQSLVVRRL
                     VADLNLGLDIVVLPTVRESDGLALSSRNVLLNPEERRQATILSRALFQAQAVVGKGER
                     DAAQLRAAIETQIQSVPLAKLDYVGVCDPDTLEPLTQIAGRAVVLVSASFGEIRLIDN
                     ILLEP"
     gene            4134..4679
                     /locus_tag="AC16G0_17965"
     CDS             4134..4679
                     /locus_tag="AC16G0_17965"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017285962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5101545.1"
                     /translation="MFQVPEILATKRLVLRRPRMSDAVAIFAEYAQDAEVTKYLVWRP
                     HREIKETADFLAGCLARWESGEELTWGLTQKNVDRVIGMVACRMRGHAADIGYVLARE
                     YWRRGYATEAVRVVVEWVGSLAPVFRVWAVCDTANAASARVLEKVGMSREGILRRWIM
                     HPNVSSEPRDCLVYSKVRDEV"
     gene            4725..5642
                     /locus_tag="AC16G0_17970"
     CDS             4725..5642
                     /locus_tag="AC16G0_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR5101546.1"
                     /translation="MERMKATDFDPQLLRLFDKYVHGHISRREFLDGASKFAVGGLTA
                     TALWEMLRPNYALAQQVAKDDKRIKAEYAMYPSPQGNASDGMMRGLLARPATGDKFPA
                     VVVIHENRGLNPYIEDVVRRLAVAGFMALGPDALWPLGGYPPGDKYGAEADEKAVAMQ
                     RTLDGKKITEDFVAAANFLLKHSQSTGKLGAVGFCFGGGMVNTLAVRLPQLAAGVPFY
                     GAAPPVEDVPKIKAALLLHFAETDPGINGRWPAYEAALRANGVRYEAYVYPGTNHGFH
                     NDTTPRYDDAAAKLAWQRTTDHFNKTLKS"
     gene            5943..6602
                     /locus_tag="AC16G0_17975"
     CDS             5943..6602
                     /locus_tag="AC16G0_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011500268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HIR5101547.1"
                     /translation="MSSNQAKPKIVCLPNGPYYLLNDMTSVAVPNLRRANGEPCASVR
                     GVALCRCGGSKNKPFCDGTHGTIGFKDQNVADKNQNRRVTYVGKRISIHDNRAICAHA
                     GFCTDSLGTVFRSQQEPWIDPDGASVDQIMETIRRCPSGALSYSIDNVEARDPERAPM
                     VTVTNDGPYAVTGGIELVGIPFGEGASTEHYTLCRCGASKNKPLCDGSHWEVGFRDPV
                     G"
     gene            7027..7434
                     /locus_tag="AC16G0_17980"
     CDS             7027..7434
                     /locus_tag="AC16G0_17980"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR5101548.1"
                     /translation="MVEIDVTYRGGLRCEAVHGPSGQRLVTDAPVDNRGKGESFSPTD
                     LVATALGTCIPTIMGIVAEREKIDLTGLRVTVTKEMAAEPPRRIAKLTTRIVMPRGLD
                     ERQKARLEKAAHTCPVHPTLQGNVDMPIEFVYS"
BASE COUNT         1455 a         2356 c         2527 g         1393 t
ORIGIN      
        1 gggccgcggg ctgggtcgtg ggaaaggcgc ccggtctgta tgatatgctg gatgtcttgg
       61 gactgaagaa gtagagcggc gggctgagaa gcacagggca cggggcacag ggcaaagcgc
      121 agagggcaaa gagcgggagg cgagagagag cgcggcaccc gctgttgatt atcgaaattc
      181 aaggagagca agcttatggg tgggcatgtg caggcgcggc gactccaggt tcttcccccg
      241 tatgtgttcg ccgagttgga ccgaatgaag caggagcagg tgaagagggg ggtggacatc
      301 atcagcctgg ggatcggcga cccggacctg ccgacgcccc cgcacatcat ccaggccctg
      361 gcgatggctg ctgccgatcc acggaatcac cagtacccat cctacgaagg gatgctgagc
      421 ttccggaagg cggtggccga ctggtatcag gctcgcttcg gcgttgctct ggatccaaac
      481 aaggaggtcc tgaccctgat cggctcgaag gagggggtcg ggcaccttcc gctggccctc
      541 atcgatcccg gcgacgtggt cctggtcccc gatcccgcct accctgtgta ccaagcgggc
      601 accctctttg ccggcggcga agccttcccc ttgccgctca cccgcgagcg cggcttcctc
      661 cccgacctgg acgccgttcc ggctgacgtc ctcggacggg ccaggatcct gtggctgaat
      721 tatccgaaca atccaacggg cgcgattgcg ccccgggagt tcctggccaa ggctgtggag
      781 ttcgcccgcc ggcaccgatt gatcctggcc cacgatgcgc cctactccga gctggccttc
      841 gacggctacc gccccgagag catccttagc atcgaggggg caaaagaggt cgcgatcgag
      901 tttcactcgg tctcgaagac gtacaacatg accgggtgga gactggcatt cgccgtgggg
      961 aatgcggaga tcctggcggg acttgggcgg atcaagcaga atctcgattc gggggtcttc
     1021 caggcggtgc agtttgccgg catcgccgcc ttgaccggtc cccagcagtg cgtcgcggag
     1081 aactgccgca tctaccagga acggcgggac gtgctcgtca aaggcttccg agagatggga
     1141 ttcgaagtgg cctcacccaa ggcgacgttc tacctctggc tgccggtgcc caagggcttc
     1201 acgtccgcca gcttcaccgc cgaactactg gtgaaggcag gcgtcgtggt cacgccgggg
     1261 agcgggttcg gcgccgcggg ggagggctac gtccgctcca ccttcaccgt cagcgtggac
     1321 cgcattcgcg aggcggtgga acggatcaag aagctgggga tccggggcgg ctgaggtagg
     1381 gtgttcggcg gttcggccgt actgtcgggc cgccgttcga tttgctcgcc atgcaggacc
     1441 gcatcctgat tcagggcatc cagttctacg ggtaccatgg cgttcgtgcg gaagagcgga
     1501 agctcggcca gctcttccag gcggacgtgg agctgcgctt ggaccttcgt gccgcgggcc
     1561 agcgggacga cctcgcggcg agcgtggact acgggaaggt gagcgccgcg gtgatggacg
     1621 tcggcaccca cgatccatgc aagctcctcg aaaggctagc cgagcggatc gcgacggtcc
     1681 tcctcgagcg gttttccgtg gagcaggtca cggtccgggt cacgaagcct catccgccga
     1741 tgtccaacgt gatcggtggc gtctctgtgg agatcacgcg gccgtgagcg tgcaggcgta
     1801 tatcgccatc ggctccaacc tgggcgatcg ccgggcgaat tgccaccagg ccctggaccg
     1861 tctcgggcgt ctccccggga cgaccctgct gcgggtctcc tctctcatcg agacgcagcc
     1921 cgaggagggc gtagccggcg gagctttcct gaacggggtg gcggagatcg tgacggagct
     1981 ggctccttgc ccgctcctga atcacttgca ggcgatcgag gtctcactgg ggcgagtggc
     2041 cccgcatggg cgaggaaccg cccggacgat ggatttggac ctcttgctct acggggacct
     2101 ggtgatgcac gaggccgacc tcagcatccc ccatccccgc atggcctcgc ggcggttcgt
     2161 cctcgcgccc cttgcggagc tggcgcccgg gcttcaccat cctgttctca gagtcatgat
     2221 ccaggaactc ctgcgtcgcc tggatccgac cgggtgcgtt tcgccctcgg gggtgccggc
     2281 atgaaaggga ctccgacctc gccccgctac attgtggtgg aaggtcccat cggtgtgggg
     2341 aagacgagtc tcgtggagcg actctcccac cgcctggggg cgcgcacgct tctggaggtg
     2401 gtggaggaca atccattcct cggagagttc tacaaggatc cacacaggta cgccttccag
     2461 acccaactct ggttcctgtt gaatcgttac aagcagcagc aggagctggc gcagttcgac
     2521 ctgttcggtc agaccctggt caccgactac ttgtttgcca aagacaagat cttcgcctat
     2581 gtgaacctgg aggatcacga attggccttg tacgagcgca tccacgccct cttggaggtg
     2641 cgcgtgccca cgcccgactt ggtgatcttt ctccaggcga gcaccgaggc tctgctctca
     2701 cggatcggaa cacggggcaa gccatacgag cgggggatca ccgccaagta tctggaggat
     2761 ctgaacgcgg cctacaccca tttcttcttc cactacacgg cgtcgccgct cctggtggtg
     2821 attaccagcg acatcgactt cgtcaaccgc ccggaggatc tcgaggatct ggtcaaagag
     2881 atcgggcaaa cagggggagg gacacactac tacgtcccgc ttggatcccg cagataggac
     2941 aaccgggacc gagacggcag acgggccgga aatctcatcc gcaggatcga cggataccgg
     3001 agccgcaccg ggaccgagcg ccattggaga ccgagcgagg agtacaccgt cgtcatgggc
     3061 cctccggagc tgtccggagg gcccatgtgc ttgttccgga aggtatccgt tcgcgaccga
     3121 acgccgttag ccattggcca ggggtgcgtg ggatgcagat cattcaaggc cctgccgaga
     3181 tgcagcgaat ggtcgaggaa tctcgccagg cggggaagtc catgggcctg gtcccaacca
     3241 tgggggcgct gcacgatggg cacctcagcc tgatccggcg atgtcgggcc gagaacgacg
     3301 tggtcctcat gagtctgttc gtgaacccga cccaattcaa tcggaaggac gatctggacc
     3361 gctatccgcg ggacctcgac cgggacgcgc gcctggcccg ctccgctggc gtggacgtca
     3421 tcttcgcccc ggcagtggaa ggaatctatc cggaggggtt cgcgacctac gtcagcgtgg
     3481 agcgcctgac cgaccggtgg gagggggcgg cgcgtcccgg ccatttccgc ggcgtcgcca
     3541 cggtgtgcgc caaactgttc acaatctgcc gtccccaccg cgcgtatttc ggccagaagg
     3601 attaccagca gtccctggtg gtccgccgcc tggtcgccga tctcaacctt ggcctggaca
     3661 tcgtggtgct gcccacggtc cgcgaatcgg acgggttggc cctcagttcc cggaacgtcc
     3721 tgctgaatcc cgaggaacgc cggcaggcca ccatcctgtc gcgggcgctc ttccaggccc
     3781 aggccgtggt cgggaagggc gagcgtgacg ccgcgcagct ccgcgccgcc atcgagacgc
     3841 aaatccagag tgtgccactg gccaagctcg actacgtcgg ggtctgcgac cccgacacgc
     3901 tggagcccct cacccagatc gcgggccgcg ccgtcgttct cgtctccgcc agcttcggcg
     3961 aaatccgcct gatcgacaac atcctgctag agccgtagta tatccccgat cacctgtcct
     4021 gtcctcctgg cgaatccatg ggttgcgaga atctgtcaac gaatcgtttc agggggcccg
     4081 ccgcagtgac ccctgagcgt cggcgtcata tctcgcagag gatgacagag ttcatgttcc
     4141 aggttcccga gatattggcc accaagcggc tggtcttgcg tcgtccccga atgtcggatg
     4201 cggtcgccat ctttgcggag tacgctcaag atgcagaggt gaccaagtat ctggtctggc
     4261 gcccgcaccg tgagatcaag gagacagcag acttcctggc gggatgcctg gcgcggtggg
     4321 agtccggtga ggagttgact tggggtctca cccaaaagaa cgtcgatcgc gtgatcggga
     4381 tggttgcgtg tcggatgcgt ggacacgcag cggatattgg gtatgtcctg gcccgggagt
     4441 attggcgccg aggctacgcg acagaggcgg ttcgtgtggt ggtcgaatgg gtaggctcct
     4501 tggcgccagt gttccgcgtg tgggcagtgt gtgataccgc caatgcggct tcagctcgtg
     4561 tgctagagaa agtgggaatg agccgagagg gaatactccg ccgatggatt atgcacccga
     4621 acgtttcctc agagccccgg gattgtcttg tgtactccaa agtgcgtgac gaggtctagg
     4681 agggcgttca cacctatcgc aggaatctgc aaggagtcaa tcagatggaa agaatgaagg
     4741 cgaccgattt cgatccccaa ctgcttcggc tcttcgacaa gtacgttcat ggccacatca
     4801 gccgccgtga gttcctggat ggtgcgtcaa agttcgccgt tggcggcctg acggcaacgg
     4861 cattgtggga gatgctccga cccaactatg ccttggcgca gcaggtggcc aaggacgaca
     4921 agcggatcaa ggccgagtat gcgatgtacc cctcgccgca gggcaacgcg tccgatggaa
     4981 tgatgcgagg tttacttgcc cggcccgcaa ccggcgacaa attccccgcc gtggtggtga
     5041 tccacgaaaa ccgcggactg aatccgtata tagaggatgt cgtccggcgc ctggccgtcg
     5101 ccggcttcat ggctcttggg ccggatgccc tgtggcctct cggcggctat ccgcctggcg
     5161 acaagtacgg agccgaggcc gatgaaaaag cggttgcgat gcagaggacg ctggatggca
     5221 agaaaataac cgaggacttc gtggctgcgg caaacttcct gttgaagcac tcacaatcga
     5281 ccggtaagct cggtgccgtg ggcttctgct tcggcggcgg tatggtcaac acgctggctg
     5341 ttcggttgcc gcagttggct gccggggtcc ccttctacgg cgccgcgccg cccgttgagg
     5401 acgtgccaaa gatcaaggcc gctcttctgc ttcactttgc agagacagat cccggcatca
     5461 acggcaggtg gcccgcctat gaggccgccc tcagggcgaa cggcgtcagg tacgaggcgt
     5521 atgtgtatcc ggggacgaat cacggtttcc acaacgacac gacaccccgg tatgacgatg
     5581 ccgcagccaa gctcgcctgg caacgcacca ccgaccactt caacaagacg ttgaaatcgt
     5641 agaatccgtt tggtccgagg gatcggcagg tgcgaaacgc tgacacccaa gtacctcatc
     5701 acggcaaatc gaataacacg tgaatcgcca agacgcccag gtcgccaaga atgttccaat
     5761 atcgcccagg ctgctcaaac aggtccagca aggcggcgca cccatgtctc gatgggtatc
     5821 cggtgcggtt gggggcgcac ttactctgta cgccgcagcg ccacgcgagc gcgccgggta
     5881 cccatccgga ggatgggtgt cgccaggtgg gcctttttcg gcaacctgct gagaggtgaa
     5941 gcatgagctc gaatcaggcg aaacccaaga ttgtgtgctt gcccaatggc ccctattacc
     6001 tgctgaacga catgacgtcc gtcgcggtgc caaacctgcg ccgcgccaac ggtgagccgt
     6061 gcgccagtgt gcgcggggtg gcgctgtgcc gctgcggcgg ctcgaagaac aagccgttct
     6121 gcgatggaac gcatggcacg atcggcttca aggaccagaa tgtcgccgac aagaatcaga
     6181 atcgtcgagt gacctacgtg gggaaacgca tcagcattca tgacaatcgc gccatctgcg
     6241 cgcacgccgg cttctgcacc gacagcctgg ggacagtgtt ccggtctcag caagagccct
     6301 ggatcgaccc ggacggcgcc agcgtcgacc aaatcatgga aaccatcagg cgatgcccgt
     6361 ccggcgcact gagctactcc atcgacaatg ttgaggcccg agatccagag cgtgcaccga
     6421 tggtgacggt cacgaacgac ggcccctatg cagtcaccgg gggcatcgag ctggttggca
     6481 tcccattcgg cgagggggcc tccaccgaac actacacgct ctgtcgctgc ggtgcgtcca
     6541 agaataaacc cttatgcgat ggctcgcact gggaggttgg attccgggat ccagtcggct
     6601 gatggtctgt gcggtctgat cgcagatgtc gcccgacacg tctatcagcc ggactactca
     6661 aaggctgagc cgtttggccg ccggttgtca tcgtaggtga gcgtgggtcg gaacgcggtc
     6721 gcgccaaggg ggcggacggc cccgagccat acaagcccgc ggcacacctg cgcggcacag
     6781 acgtccgcac gtcggaagag atgtggccgg ccgtcgcctg ccaactgacg agagaaccgt
     6841 ccacaagaga ctctccccgg tcttactatt cgtttcaaaa ccggaacatc ccgaagcaaa
     6901 tcattgaagg gctcggtcgg ggggcgtatc ctaccccgcg attgagactg tccgtattcc
     6961 cggagagcgc accggttgat ggcgcgacgt tagccaataa ccttctctgc agcgtggagg
     7021 ttccacatgg ttgagatcga tgttacctat cgcggcgggc ttcgatgtga agcggtccac
     7081 gggccgagcg gtcaaaggct cgtcaccgac gcgccggtgg acaaccgcgg aaagggggaa
     7141 agcttttccc ctacggatct cgtcgccacc gccctgggga cgtgtattcc caccatcatg
     7201 gggatcgtgg ccgaacgtga gaagatcgac ctcaccgggt tgcgggtcac tgtcaccaag
     7261 gagatggctg ccgagccacc ccggcgaatc gcgaagctca ccacccgaat cgtcatgccc
     7321 aggggactgg acgagagaca gaaggccagg ctggagaagg ccgcccacac ctgccccgtc
     7381 cacccgaccc tgcagggcaa cgtcgacatg cccatcgagt tcgtgtactc gtagactgat
     7441 cggaaatctc ccgcagtctt tcgccgtcca atgctcagcc ggaagaggaa ggtcaccagg
     7501 ctggtctgcc gaggatagtc gggatggcat cgagtccggc tgcggtgtcg ggaggtcaaa
     7561 catgccaggc gtggaggaag ccgtggggtc gaggaggaac atcgacgcga tcgcgctctg
     7621 cgacaccgac aacgttgcga cggcgttgcg ggagcttgtt gcgggggagg agatcgtggt
     7681 cggcgttctg aactgcaggg ttcatgtgcg cgtgcgccag gccatccctg t
//
LOCUS       DBNGZC010001044         1788 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1853843, whole genome shotgun sequence.
ACCESSION   DBNGZC010001044 DBNGZC010000000
VERSION     DBNGZC010001044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1853843"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="AC16G0_17985"
     CDS             complement(<1..212)
                     /locus_tag="AC16G0_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006419932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreAB"
                     /protein_id="HIR5101549.1"
                     /translation="MPGTPSPDPISTRRQRIAELARDSPQAAFTTLAHHIDTDWLLEA
                     YRRTRKDGAAGVDGQTAADYAADQGG"
     gene            1025..>1788
                     /locus_tag="AC16G0_17990"
     CDS             1025..>1788
                     /locus_tag="AC16G0_17990"
                     /inference="COORDINATES: protein motif:HMM:NF013698.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HIR5101550.1"
                     /translation="MSTSSSLDCIGVGIDTARYGHRVSFLRPDRQPAAKPLTVMENRA
                     GYQALEERLKQLRERHPQAHFHVRIDAAGQYAVNLEQFLRGLDLPMTLSIGEPKRNKD
                     YQKAHFPKRTTDDTESQAMARFAVVELPAATPSPSTPMVLLREVAGRLQAQVKQTTQA
                     INRLHNLLARAFPELATLTDDLAAGWVLRLLDKYPTADRIAQAHRATLEKIPYLPHEQ
                     AEALHQAAQRSVASLRGAVAEALVRELVTEVRHSQNA"
BASE COUNT          251 a          686 c          521 g          330 t
ORIGIN      
        1 ttgcccccct ggtcggccgc gtagtccgca gccgtctgtc cgtccacccc cgcggccccg
       61 tccttgcgcg tgcgccggta ggcctctagc agccagtcgg tgtcgatgtg atgggcgagc
      121 gtcgtgaacg ccgcctgcgg cgaatccctc gccagttccg ctatccgctg tcgtcgcgtt
      181 gagatggggt cagggctcgg tgtccctggc atcgttccct cccgcggtct cctggcctgg
      241 cgcctccttc cctcggcggg gtccctcggg tcggttcccc cgcgtcgtcg gtaccccgag
      301 gcgctccgac tccccgtcgc ccgtcccacc gcgcttcgtt gccttcgcga ggcggtacca
      361 cggctgcacc catctttcgc tcccgtggcc gccgggtgcc ccggccgcgg gcctggagtt
      421 ggtcacccgg tacctccggc cgggatttgc cgtgaaggcg gcggggcctc ccaggttcct
      481 gggggaccca cgtgagcgtg ccctgttctt cgaccccggc gggatcacga acgccaggcc
      541 cttgcggcgt cgtgatgcgg ccttccgtca actgcacgac gtcggctccc gcgagcagga
      601 tttcggggct caatcgcacg gcccgctcgc tcgctgtcta cgcttcgcag cccgagttac
      661 cccggcccca cgcaagactc gcttccggcc gctggccaag ctttaccggg cggggctggt
      721 tacccacagg gtcccgacga aaggtttccg ctacatcgct tcccccttgt atgtttcact
      781 gccggcagtt ggcttgccat actgctggca gtgtcgtgag gagagctggc ttccccctgt
      841 ggcgtcgacc tcttgggata gattgttccc tccggcttgg gctcaaggct ttggtacatc
      901 ttgcggcctg gaccgctcat cacaaggatc acctcgccca gaatctccgg aggagactca
      961 cgccgccctc tccgacacgc gttggccctg gcatctcacc gtctcctcgc gccggaggtc
     1021 tcccgtgtcc acctcctcgt cccttgactg cattggcgtc gggatcgata ccgcacggta
     1081 cggtcatcgt gtctccttcc tcaggcccga tcgccagccg gccgccaagc cgctcaccgt
     1141 gatggagaac cgcgctggct atcaggcctt ggaggaacgc ctgaaacagc tccgtgaacg
     1201 gcatccccag gctcatttcc acgtgcgcat cgatgccgct ggccagtacg ccgtcaacct
     1261 cgagcagttc ctccgcggcc tcgacctgcc catgaccctg tccatcggcg agcccaagcg
     1321 caacaaggat tatcagaagg ctcactttcc caaacgcacc accgacgaca ccgagagcca
     1381 ggccatggcc cgcttcgccg tcgtggaact ccctgccgcc acgccgtcac cctcaacgcc
     1441 catggtcctg ttgcgcgagg tcgccggccg gttgcaggcc caggttaaac agacgaccca
     1501 ggccatcaac cggctccaca acctcctggc ccgagccttc cccgaactcg ccaccctgac
     1561 cgacgacctc gccgccggtt gggtcctgcg gttgctcgac aagtatccga ccgccgaccg
     1621 catcgctcag gcccaccgcg ccacactgga gaagatcccg tacctgcccc acgaacaagc
     1681 cgaagcgttg caccaggccg cgcagcgctc ggtggcttcc ttgcgcggcg ccgtggccga
     1741 agcactggtc cgcgaactcg tgaccgaggt ccgtcacagc cagaacgc
//
LOCUS       DBNGZC010001045         1990 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_92571, whole genome shotgun sequence.
ACCESSION   DBNGZC010001045 DBNGZC010000000
VERSION     DBNGZC010001045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_92571"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="AC16G0_17995"
     CDS             <1..747
                     /locus_tag="AC16G0_17995"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015734725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5101551.1"
                     /translation="LDEKIAFVTGAGRGLGQAGAIAFARAGANAVLVSRSRSQLEETA
                     AAVERLGRKALVAPADVRKPEEVEEAVRAAVQTFGRIDILFNNAGTNVRKPVVEMTDE
                     DWHTIVETNVKGVFVVARAVARQMIAQRSGRMINMSSVTATLAERDKVVYASSKGAIA
                     QFTKGLAIELAPYGINVNAVAPGYLMTPLVKGYLEADAERYKRILARIPLSRIGKPED
                     LGGALVFLASDAASYITGATIAIDGGWAAG"
     gene            818..940
                     /locus_tag="AC16G0_18000"
     CDS             818..940
                     /locus_tag="AC16G0_18000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101552.1"
                     /translation="MTKKYIQDGEHLIELEVWVQNGKGEVTVQGTSTVRLPARS"
     gene            950..>1990
                     /locus_tag="AC16G0_18005"
     CDS             950..>1990
                     /locus_tag="AC16G0_18005"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HIR5101553.1"
                     /translation="MARSKLMNPAQAAALIQDGRTVVVEGAGRLLLSEAILTALEQRF
                     LETEKPEDLTLIQACGFGNDGEAGASHFAHAGLVKRVISPGWGDSPRMAQLALANQVE
                     AYCFPQGAVGQMIWASASKSPRVISHVGLGTFVDPRQDGGKMNAVTHEDLVEVIELDG
                     AEWLTFRVLPIDVAIVRGTTADEFGNIRMEHEPAILEPLTMALAAHASGGLVLAEVKR
                     LVRRGSLHPQAVRVPGILVDGIVVNPEAWQIYGEERYNPVFSGETKAPRRPPPDIEIS
                     GRMVVARRAAMELFPGAVINIGFGISDGIPVVATQEGLVEDLTFTIEQGTIGGTPQRG
                     RAVAAQVNVEAII"
BASE COUNT          374 a          580 c          714 g          322 t
ORIGIN      
        1 ctggatgaaa agatcgcctt tgtcaccggg gcggggcgcg gcctgggaca ggcaggggca
       61 atcgcctttg ccagggccgg cgccaacgcg gtgctggtga gccgttcgcg gagtcagctc
      121 gaggaaacgg cggcggctgt cgagcggttg gggcgcaagg ccctggtggc gcccgccgac
      181 gtgcggaagc cggaggaggt cgaggaggcc gtccgcgccg cggtgcagac cttcggacgg
      241 atcgatatcc tgttcaacaa cgccgggacc aacgtgcgca agccagtggt ggagatgacg
      301 gatgaggact ggcacaccat cgtggagacc aacgtgaagg gggtcttcgt ggtggcccgg
      361 gccgtggcga ggcagatgat cgcccagcgg agcggtcgga tgatcaacat gtcctcggtg
      421 accgcgaccc tcgcggaacg ggacaaggtg gtgtacgcct ccagcaaagg cgccatcgcg
      481 cagttcacga aggggctggc aatcgagctg gcgccttacg ggatcaacgt gaatgccgtc
      541 gctcccggct atctgatgac tcctttggtg aaagggtatt tggaagccga cgccgaacgg
      601 tacaagcgca ttctcgcgag gatcccgctc tcgagaatcg gaaagccgga ggatcttggc
      661 ggggccctgg tattcctggc ctcggacgcc gccagctaca tcaccggcgc caccatcgcc
      721 atcgacgggg gctgggcggc cggataggtc gcggcgagct tgcgggcgag aacgggaagt
      781 acggtggatc gggcatgcct tgacccgtca agggaaggtg acgaagaaat acatccagga
      841 cggcgagcac ctaatcgaac tggaggtctg ggtgcaaaac gggaagggag aggtcacggt
      901 ccaggggacc tcgaccgtcc ggctcccggc gcgaagttag gagatgcgga tggcccgttc
      961 gaagctgatg aatccggcgc aggcggcggc cctcatccaa gacggtagga ccgtggtcgt
     1021 cgagggggcc ggccgtctgt tactctctga ggcgatcttg accgctctgg agcaaagatt
     1081 cttggagacc gaaaagccgg aagacctcac ccttatccaa gcgtgcggct tcgggaatga
     1141 cggggaagca ggcgcgagcc attttgcgca tgccggactc gtgaagcgtg tgatctcccc
     1201 cggctggggg gattctccca ggatggccca gctcgccctt gccaatcaag ttgaggcgta
     1261 ttgtttcccc cagggcgcgg tgggccagat gatctgggcg agcgccagca agagcccccg
     1321 cgtgatttcc cacgtggggc tgggcacctt cgtggacccc cgccaggacg gcgggaaaat
     1381 gaatgccgtg actcacgagg acctggtgga ggtcatcgag ctagacggtg ccgagtggct
     1441 caccttccgc gtcctaccga ttgatgtcgc catcgtccgg gggaccacgg ccgacgagtt
     1501 cggcaacatc agaatggagc acgagccagc catcctggag ccgctgacca tggccctggc
     1561 agcgcacgca tccggcggcc tcgtgttggc cgaggtcaag cggctcgtgc gacggggcag
     1621 tctgcacccg caggccgtca gggtgcccgg catcctggtg gatgggatcg tggtgaatcc
     1681 ggaggcatgg cagatctatg gagaggagcg gtacaacccc gtcttttcgg gggaaaccaa
     1741 ggcgccccgg aggcctcccc ccgacataga aatctccggg cggatggtcg tggcccgtcg
     1801 cgccgccatg gaattgtttc ctggagcggt tatcaacatc ggcttcggga tctcggacgg
     1861 catccccgtc gtggccacac aagaaggact cgtcgaggat ctcaccttca cgatcgagca
     1921 ggggacgatc ggtgggacgc cgcagcgcgg gcgggcggtt gctgcccagg tcaacgtgga
     1981 ggcgatcatc
//
LOCUS       DBNGZC010001046         1914 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2242661, whole genome shotgun sequence.
ACCESSION   DBNGZC010001046 DBNGZC010000000
VERSION     DBNGZC010001046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2242661"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="AC16G0_18010"
     CDS             <1..503
                     /locus_tag="AC16G0_18010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HIR5101554.1"
                     /translation="CAPHYKRVQVQERKHARRAGAGGPPPAAHPAHGFSRGCMAGYGF
                     CFISHIGEVGGCGYLPLLAGSVRQSSLVEVYRESPLFKSIRDANLLQGRCGICEYRVL
                     CGGCRARALGASGNYLEEEPFCTYQPDPRVPRELVNVEALVSAAAAHEIRTEAPPPKD
                     FIPPAF"
     gene            631..1614
                     /gene="hemB"
                     /locus_tag="AC16G0_18015"
     CDS             631..1614
                     /gene="hemB"
                     /locus_tag="AC16G0_18015"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HIR5101555.1"
                     /translation="MHYPIYRPRRMRETETIRRMVRETRLTLDAFIMPFFVTHGSGVR
                     REVDSMPGNYQLSVDELVREVKETAALGIPGVLLFGLPAVKDAVGSEAYARDGIVQQA
                     VRAIKDSVSDLVVITDFCLCEYTSHGHCGIVERGRVKNDPTLELLAKTALSHAEAGAD
                     MVAPSDMMDGRVAAIREALDEAGHEETPILAYSAKYASGFYGPFRDAAESTPQFGDRR
                     AYQMDPANADEALREVALDLDEGADMIMVKPALPYLDVIWRVKTEFGVPVAAYNVSGE
                     FAMLKAAARLGWLDEERVMLEMLTAIKRAGADLILTYFARDAARLLRQQER"
     gene            1749..>1914
                     /locus_tag="AC16G0_18020"
     CDS             1749..>1914
                     /locus_tag="AC16G0_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008475320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR5101556.1"
                     /translation="MGDRSRQLFQEASRYFPGGVNSPVRAFRAVGGDPFFVASASGSR
                     ITDVDGKSYID"
BASE COUNT          313 a          613 c          656 g          332 t
ORIGIN      
        1 cctgcgcgcc gcattacaag cgggtccagg tgcaggagcg aaaacacgcc cgccgtgcgg
       61 gggccggggg tccgccgccg gccgcccatc cggcgcacgg gttcagccgg gggtgcatgg
      121 ccggctatgg attctgcttc atctcccaca tcggggaggt gggcggttgc ggctacctgc
      181 ccctcttggc ggggagcgtt cgccagtcct ccctggtgga ggtctaccgc gaatccccac
      241 tgttcaagag tatccgggac gcaaatctcc tccaggggcg ctgcggcatc tgcgagtacc
      301 gggtgctgtg cggcggatgt cgggcccgcg ccctgggagc ctcggggaac tacctggagg
      361 aggagccctt ctgcacatac cagcctgacc cgcgggtccc gcgggaactg gtcaacgtgg
      421 aggcgctggt ctcggccgcg gcggcgcacg agatccggac agaggcgccc ccgcccaagg
      481 acttcattcc cccggccttc tgaggggggt cggcaagagc cgcccgcgcc acttgagatc
      541 gggcaagcga catttgacca ctgaccacca cccgctgatt acggacggtt ggctgtccgt
      601 attccattcg tcatttcaac ggagttgacc atgcattacc ccatctatcg accccgacgg
      661 atgcgcgaga ccgagacgat ccgccgcatg gtgcgcgaga cgcgcctcac gctggatgcg
      721 ttcatcatgc ccttcttcgt gacccacggc agcggcgtgc gccgcgaggt cgacagcatg
      781 ccggggaatt atcagctttc cgtggatgaa ctggtgcgcg aggtgaagga gacggcggcg
      841 ctgggcatcc ccggggtcct cctcttcggg ctccccgcgg tgaaggatgc ggtggggtcc
      901 gaggcctacg cccgcgacgg catcgtccag caggcggtcc gggcgatcaa ggattcggtc
      961 tctgacctcg tggtcatcac cgacttctgt ctctgcgagt acaccagtca cggccattgc
     1021 ggcatcgtcg agcgcggtcg ggtcaagaac gaccccacgc tggagctctt ggccaagacc
     1081 gccctctcgc acgccgaggc gggcgcggat atggtggcgc cctcggatat gatggacggc
     1141 cgggtggcgg ccatccgcga ggcgttggac gaggcgggcc atgaggaaac acccatcctg
     1201 gcgtactccg caaagtatgc cagcgggttc tacgggccgt tccgcgatgc ggcggagtct
     1261 acgccccagt tcggcgatcg ccgggcgtac cagatggacc cggccaatgc cgacgaggcg
     1321 ctgcgcgaag tggccctgga cttggacgag ggagcggaca tgatcatggt caagcccgcc
     1381 ctcccctacc tggacgtcat ctggcgcgtg aagacggagt tcggcgtgcc cgtggccgcc
     1441 tacaacgtga gcggagaatt cgccatgctg aaggcggcgg cacgcctcgg ctggctggac
     1501 gaagagcgag tcatgctgga gatgctgacc gccatcaagc gggccggtgc tgacctgatc
     1561 ctgacctact tcgcgcggga cgccgcgcga ctcctgcggc agcaggaacg gtaagccgcg
     1621 ggtgggcgag gtcacctgcc tcgatttatt tcccccaaaa ttcttgtgag ggcttgacag
     1681 gacgggagag tctcgcgttt aatagccgcg ctcgccacca agcagtccgc cgtcgggagg
     1741 gggatttcat gggggatcgt tccagacagt tgttccagga ggcctcccgt tattttccgg
     1801 gtggagtgaa tagccctgtc cgggccttcc gcgcagtggg gggcgatccg ttcttcgtcg
     1861 cgtcggcctc cggcagccga atcaccgacg tggacggcaa gagctatatc gact
//
LOCUS       DBNGZC010001047         1915 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_972979, whole genome shotgun sequence.
ACCESSION   DBNGZC010001047 DBNGZC010000000
VERSION     DBNGZC010001047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_972979"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="AC16G0_18025"
     CDS             <1..951
                     /locus_tag="AC16G0_18025"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase family protein"
                     /protein_id="HIR5101557.1"
                     /translation="YHHYRGERWRFKVVSRRGAFHGVTGGALRVLGTTLPARQMMEPL
                     PPGTIFAESPYCYRCPLHLTYPACDIACARDVERLIQFEEPAQVMAFIGEPIQQGFGA
                     CRPPREYWPIIREICDRYGILLIVDEVICGFGRTGEWFGIQHFDIQPDIMLMAKGISS
                     GYVPLGAVGATEAVVGPVENFANLQTYMNHPVACAAGLKNIEILKRENLIQNARAMGE
                     YFLEGLRSLLRHPAVGEVRGTGLWTALDLTTDRTTRAGFPADRLASIVDRARQRGLII
                     KSMGMALEFAPPLVITRPDIDEAVRILDECLTEEETAMAL"
     gene            1422..>1915
                     /locus_tag="AC16G0_18030"
     CDS             1422..>1915
                     /locus_tag="AC16G0_18030"
                     /inference="COORDINATES: protein motif:HMM:NF021997.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HIR5101558.1"
                     /translation="MDKKTQRKVSRRDFLKVGAAGLGVAALGSLPGKAFSAAPAVIKG
                     TKLTILQGTYFIAPGQDLYKKQAAEWGQENGVEMSADFLNWPDLQPKIAAAIQGGGVD
                     IVELWPSWNHLYKNNLVDMTKEAEEFGKRGGGFEKYVLNSAKVGNRWLGIPHGMSNAS
                     MAYR"
BASE COUNT          355 a          587 c          653 g          320 t
ORIGIN      
        1 taccatcact accggggcga gcggtggcgg ttcaaggtgg tctcacgacg gggggccttc
       61 cacggcgtca cgggcggcgc cctgcgggtg ctcggcacca cactcccggc acgtcagatg
      121 atggagccgc tcccgcccgg caccatcttc gccgagtcgc cgtactgcta ccgctgcccc
      181 ctgcacctca cctacccggc ctgcgacatc gcctgcgcgc gggacgtgga gcgcctcatc
      241 cagttcgagg agccggccca ggtcatggcc ttcatcgggg agcccatcca gcagggcttc
      301 ggggcgtgcc gcccacccag ggaatactgg cctatcatcc gggagatctg cgaccggtac
      361 ggcatcctgc tcatcgtgga cgaggtgatc tgcggcttcg gtcggacggg cgaatggttc
      421 ggcatccagc atttcgacat ccagccggac atcatgctca tggccaaggg gatctccagt
      481 gggtacgtgc ccctgggggc ggtgggggcc accgaggcag tcgtcgggcc cgtggagaat
      541 ttcgccaatc tccagaccta catgaatcac ccggtggcct gcgcagcggg gctcaagaac
      601 atcgagatcc tgaagcggga gaacctgatc cagaatgccc gcgccatggg cgagtacttc
      661 ctggaggggc tgcggtcgct cctgcgccat cccgcggtcg gcgaggtgcg ggggaccggt
      721 ctctggactg ccctggacct cacgacggac cggaccaccc gcgcgggatt cccggcggac
      781 cgcctggcga gcatcgtgga ccgggcgcgg cagcggggac tcatcatcaa gtccatgggc
      841 atggcgctgg agttcgcccc tccgctggtc atcacgcggc cggacatcga cgaggcggtc
      901 cggatcctgg atgagtgcct gacagaggaa gaaacggcga tggccctctg agcccgacgg
      961 gctctgaggt aaggcgaccc aatggtctgg gccgggggat gacagccaga atcacgttga
     1021 caccgcccct ccatgagagg tagcgtgtcc tcatccggca agggccccca ctgtggcttg
     1081 gaaaggacag acatgccggt gagggaaatc tctcccgtat gtgcgagcga ccccctgggg
     1141 aggtgcgtcc cggagggtcg ttgtgcttct tggcaacccc gcccctcagc cacgacctcg
     1201 acgaggcgtg ccgtcgggca gctctccaag ccattatgcc gcagctaatc gaatgcgatt
     1261 gcactgcact ccgtccaatt tatactccca tcctgaccgc cgggaatggt ggtcagtctg
     1321 gaaatgaagg ggggtgaggg agactcaaag gttggtgcaa gaggaggggc cttatatatg
     1381 gggaggggcc ttttttatac cgtgttgaaa ggaggcgctg gatggacaag aagacacaac
     1441 ggaaggtgag tcggcgcgac ttcctgaagg tgggagccgc cggcctgggc gtggccgcgc
     1501 ttggatcact gcccggcaag gcgttcagtg cggcgcccgc cgtcatcaag gggaccaagc
     1561 tgacgatcct gcaggggacg tacttcattg cccccgggca ggatctctac aagaagcaag
     1621 ccgcagagtg gggacaggag aacggcgtcg agatgagcgc ggatttcctg aactggccgg
     1681 accttcagcc caagatcgcg gcggccatcc agggtggtgg cgtggacatc gtggagctgt
     1741 ggccctcctg gaaccacctg tacaagaaca acctggtgga tatgaccaag gaggccgagg
     1801 agtttggaaa gcggggcggc ggcttcgaga agtacgtcct gaactccgcc aaggtgggca
     1861 accgctggct cggcattcct cacgggatgt cgaacgcatc catggcctac cggat
//
LOCUS       DBNGZC010001048         7562 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_775129, whole genome shotgun sequence.
ACCESSION   DBNGZC010001048 DBNGZC010000000
VERSION     DBNGZC010001048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7562)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7562)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7562
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_775129"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..755
                     /locus_tag="AC16G0_18035"
     CDS             255..755
                     /locus_tag="AC16G0_18035"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR5101559.1"
                     /translation="MANLTMKKADIIAVVIVIPICLYVFYESTKWPVPALLGRPFVIP
                     RGVAAFLLVAVGILLYRAVKGQAFPLESPLVGADLRRVSLTAVLTFGYLPLLERIGFI
                     GTTFLYMLLFAWVLGERRKPRLILFAIIVPLVMYTLFSTALHVPLPRGLIENALHEHG
                     FAVIIR"
     gene            777..2327
                     /locus_tag="AC16G0_18040"
     CDS             777..2327
                     /locus_tag="AC16G0_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009832686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR5101560.1"
                     /translation="MVDLALLFQGFATVLSGYHIVALVLGSFFGIIIGAIPGLTATMG
                     IALLVPFTFGMNPFTGIVMLLGIYSGAIYGGGIASILIKTPGTPAAAATVFDGYPLAQ
                     KGAAGKAIGMATVASGIGGTFTALCLTFLAPILANFALRFSAPEYFALAIFGLSVTVT
                     LTGKSPIKGIISGCLGLLIAMIGLDPMGGFPRFTFGTTQLTGGFSFVPMLIGLFALSE
                     GFRQVEVILTAPRVSAVLTNILPKLRELRECSVTFVRSCIIGLIVGITPAIGAETACF
                     VSYSEAKRTSKHPELFGTGILEGVAAPQCAENASTGGDLLPMLTLGLPGDAATAVLMG
                     ALTIHNLQPGPLLFRDHSDLVHQIFAGMITANVVFVILGLTFARFFAKVINIDRRFLV
                     PLIFIACMIGSYAIDNDIFDTITCVFFGFIGYVMIRYDYPVSPMVLAQILGVMMESNF
                     RRSMAMSQDNLLILVTRPIALTILILAAFTTITGIRRQRKALQMEAAMAAEAAAKAAA
                     VLAADRHN"
     gene            2848..4707
                     /locus_tag="AC16G0_18045"
     CDS             2848..4707
                     /locus_tag="AC16G0_18045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5101561.1"
                     /translation="MRGFRRLLGRSLRFKLAAGVILILIVAMGLVFAVQYHWFYQEMI
                     DRLGLSSTPLSDVIKGSLTHAMQTRNLSEVRAIIDNVSRQEGVIKVFVVDKKGMIKFS
                     PVAEEIGTSVALTDPTCQLCHRVGAESRNKTVIFTAAGGQRVFRNVNPIANEPACFGC
                     HDPKGRLNGVLISDFSMTEVDRRLASRFREMVLGLLLVVGATGLTITLTMNRLVIGKL
                     EQVVRATKLLGRGKLDLRLDVASRDEIGELATSFNEMVEGLRRAQALRERKELLESVL
                     NNVNDCVIVFAPDGSVVALNGGTERVFGLPAAEVVGRRYAFFGEDQEALLARARSGEP
                     VNLELKLQAEDGRSFPAHVYLAPLRGEGEDLLACVAVAQDLTEEKAKERLQLQLVHSE
                     KLAAVGRLAAGVAHELNNPLGNALLYAKLLLEDASPSELGRANARRIVENTLRCKAIV
                     KSLLDYAKQSEVQLTWTDLNEVIQGSVDLVANELRLHRVRCEVQLDRGLPNVRCDRRQ
                     IQQVLVNLLQNGIEAVEGQGRLMIFSRRTENGDGVVVGVRDDGPGIPSEALSRVFEPF
                     YTTKEQGTGLGLAICYGIVARHHGRIWVESSEDGGAPGSTFFVELPLAAEGQA"
     gene            4704..6125
                     /locus_tag="AC16G0_18050"
     CDS             4704..6125
                     /locus_tag="AC16G0_18050"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR5101562.1"
                     /translation="MNGDRVGEQNLIGRSVLLVDDERDALDACSQALGKTRYLVETAS
                     DPLEALERVRARSYDAAVLDLKLPQMSGIELLRAIRKIDPEVAVILVTGYATIETAVQ
                     AMQEGASDYLAKPFTPDDLRLGVRKAIERQDLLRENRELRERLGPRGEAPALLWSSQK
                     IQEVRRLIARVGATDSTVLIYGETGTGKELVARQIHAASRRASQPLIAVDCAAIPSEL
                     LESEFFGHEKGAFTGAIRRRKGSFELAHGSTLFLDEIGNMGLDLQGKILRALQEREVQ
                     PVGSERKIPVDVRILAATNRHLREAMEQGGFREDLYYRLNVVPLFLPPLRDRKEDIPL
                     LVQHFLDKYASKLNRHIQRVEPDAMRILCQYAWPGNVRELESAIERAMTLTDGAVLEA
                     EAFRHLAWETERSPKPAGSKVKPGRDEADANLPPLHQVERDYILEVLQATRWNRKEAS
                     KILGISTVTLWRKIGGKTTDDSK"
     gene            6303..6752
                     /locus_tag="AC16G0_18055"
     CDS             6303..6752
                     /locus_tag="AC16G0_18055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5101563.1"
                     /translation="MNAHIAPPCSDPVAPGTRRRLMVVDDALESRQALTEYFVARGYE
                     VSLATDGIQALAKTLTSAVDVVIMKATLPGLEGYEAAAILRKIAPRVQVILTMEAGVE
                     AQPPEQRRTERFRCFPTPLNLPEIAGAVEGAWAGTAPDLEREPGDPG"
     gene            6749..7411
                     /locus_tag="AC16G0_18060"
     CDS             6749..7411
                     /locus_tag="AC16G0_18060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5101564.1"
                     /translation="MSGRRTLAVLLIGAGACTAWLGDRRPAMAASADPEAGRAVFARK
                     QCVRCHAPRGEAGAGPPLEELRRPQGAMEMAGRLWNHVPAMSAALLQGGLGWPEINAA
                     EMTNLMAYLLADPTRDPAPDPAKGYVALFKKQCLKCHSFRREGGRVEPDLANRRPDYE
                     SAAAWAATMWTHTPRMAEMASRQQILFPRFSGDEMGNLLAFLRGGAVTAPATGPGTAP
                     GR"
BASE COUNT         1279 a         2411 c         2483 g         1389 t
ORIGIN      
        1 cgacgggttc cgcaaggccg ctgaggatcc ggaatacatc aagctcatgg atgacttgac
       61 gcttccccgc gcgtacatgg agtacgacaa gttcggggag ttcctgggag ggctcgagga
      121 cagtttgaag cctgttctgg accaggtggg tctcctgaag aagtaagctc gttccacgca
      181 gtcatggccg ggagggagag gcgatactct tcctcccggc actacggcaa tcccagagca
      241 tcgcggagcc tcctatggcg aacttgacca tgaagaaggc agacatcatc gccgtcgtga
      301 ttgtcattcc gatctgtttg tatgtgttct acgagtcgac caagtggcct gtgccggcgc
      361 tgcttggccg gcctttcgtg attccgcgag gggtcgcggc cttcctcctg gtcgccgtcg
      421 ggatcctcct gtatcgcgcg gtgaagggtc aggcgtttcc tctggagagc ccactggtgg
      481 gggcggatct tcgccgggtg agcctgacgg cggtactgac cttcgggtac ttgcccctct
      541 tggagagaat cggattcatc ggcacgacct tcctctatat gctgcttttc gcctgggtgc
      601 tgggcgagcg ccgcaagccc cggctgattc tctttgcaat catcgtcccg cttgtcatgt
      661 acacgctctt ttccaccgcc ctgcacgtcc cgctgccacg cgggttgatc gaaaacgccc
      721 tgcacgagca cggctttgcc gtcatcatcc ggtagcattt acctgggagc ccttgcatgg
      781 tcgaccttgc cctgctgttc caaggcttcg cgacggtatt gtcgggctac catatcgtgg
      841 cgctggtgct gggctccttc ttcggcatca tcatcggagc cattcccgga ctgaccgcaa
      901 ccatggggat cgccctcctg gtccccttta ccttcggtat gaaccccttc accgggatcg
      961 tgatgctcct cggcatctac tcgggtgcga tctacggcgg gggcatcgct tccatcctca
     1021 tcaagacgcc ggggaccccg gccgccgccg cgaccgtttt cgacgggtac cccctcgccc
     1081 agaagggcgc ggccggaaag gccataggca tggccacggt cgcgtcgggc atcggcggga
     1141 cattcaccgc actctgcctc accttcctcg cgccgatcct ggccaatttc gccctgcgct
     1201 tttccgcgcc cgagtatttc gccttggcga tcttcggctt gtcggtgacg gtcaccctga
     1261 ccggcaagtc tcctatcaag gggattatct cgggctgcct ggggctcctg atcgccatga
     1321 tcggtctgga tcctatggga gggttcccgc gattcacctt tgggacgacg cagttgaccg
     1381 gcggcttctc cttcgtcccg atgctgatcg gcctcttcgc cctgtccgag ggattccgcc
     1441 aagtcgaagt tattttgacc gccccgaggg tgagcgcggt cctcacaaac atcctgccca
     1501 aactgcgcga gctccgggaa tgctcggtga ccttcgtccg gtcttgcatc atcggtctga
     1561 tcgtcgggat cacgcctgcc atcggcgccg agaccgcctg ctttgtgagt tacagtgagg
     1621 ccaagcggac ctccaagcat cccgagctct tcggcaccgg gatcctggag ggcgtggccg
     1681 ccccccagtg cgccgagaac gccagcaccg gcggcgacct cctccccatg ctcacccttg
     1741 ggctgccggg cgatgccgcg accgcggtgc tcatgggggc tctcaccatc cacaacctgc
     1801 agcccggccc attgcttttc cgggatcact ctgacctggt ccaccagatc ttcgccggga
     1861 tgatcacggc gaatgtcgtg ttcgtgatcc tgggtctcac cttcgcacgg ttcttcgcca
     1921 aggtcatcaa cattgaccgc cgattcctgg ttcccctgat cttcatcgcg tgcatgatcg
     1981 gctcgtatgc catcgataac gacatcttcg acacgatcac ctgcgtgttc ttcgggttca
     2041 tcggttatgt tatgatccgc tacgactacc cggtatcgcc catggtcctg gcccagatcc
     2101 tgggcgtcat gatggaatcg aacttccgcc gctccatggc catgtcccag gataacctct
     2161 tgatcctggt gacccggccg attgcactga ccatcctgat tctggccgcc ttcacgacga
     2221 tcacgggcat ccggcgccag cggaaggcct tgcagatgga ggcggccatg gcggcagagg
     2281 ccgcggccaa ggcagcggct gtcctggcag cggataggca caactgagca ttgcatcggc
     2341 cgggaggagt catggtttga aaagggtgcc cagggtctcg cgccctcgat cggcgatgga
     2401 gacgacggga cagacgctca ccaatctgaa gctccgctga tattcttcat tcgggaggga
     2461 ggggggactt ccaaccgaca aaaagaaggc aaccaggacc gcgtggcggg tgcggagatg
     2521 tctttttccc cgccgtcggc cttccccgtg tcggtcgtcg gtgtcggctc ggaggagggt
     2581 ctccagaaat agctggtcgt ggggagatct ccgtcgtgcc cctgaggagc ccgagacggg
     2641 ccccccacgc acggcggttc accctggcca actttcggag gtgatcgaat gccggtccag
     2701 caagtccctg aagtccaccc ggcggcggag gagcacgctc cccaagagca ggcagccgat
     2761 gctcgcgccc ctggtgggcc tggtacgccc acgccgtcgc ccggtcggct cgtcgtcccc
     2821 gggggaccgg gaggctcggg gcgaagtttg agaggattcc gacggctcct gggtcggagc
     2881 ctgcgcttca agctggcggc gggcgtgatc ctgattctga ttgttgccat gggtttggtc
     2941 tttgccgttc agtaccactg gttctatcaa gagatgatcg accgcctggg cctctcctcc
     3001 acgcccttga gcgacgtgat caagggaagc ctcacccacg ccatgcagac gcggaacctc
     3061 tccgaggtca gagccatcat cgacaatgtg agccgacagg aaggggtcat caaggtattc
     3121 gtggtggaca agaagggaat gatcaagttc tctcccgtcg cggaggagat cgggacgagc
     3181 gtcgcgctga cggatccgac ctgccagctc tgccacaggg tcggcgcgga aagccggaac
     3241 aagaccgtca tctttaccgc cgccggaggc caacgagttt tccggaacgt gaacccgata
     3301 gccaatgagc cggcgtgttt tggctgtcac gatccaaagg ggaggttgaa cggcgtcctc
     3361 atcagcgatt tctccatgac tgaggtcgac cggcggcttg ccagtcggtt ccgcgagatg
     3421 gtcctgggac ttctcctggt ggtcggggcg accggactga ccatcacgct caccatgaac
     3481 cggctggtga tcggcaagct ggagcaggtc gttcgggcca ccaagcttct cgggcggggt
     3541 aagctggacc tcaggctgga tgtggcgtcc cgggacgaga tcggggaatt ggcgacctcg
     3601 ttcaacgaga tggtggaggg gctgcgccgg gcccaggcac tccgggagcg gaaggaactc
     3661 ctggagagtg tcctgaacaa cgtgaacgac tgcgtcatcg tctttgcgcc tgacggttcc
     3721 gtcgtcgccc tgaacggggg aacggagcgc gtgttcgggc tccccgcggc ggaggtcgtg
     3781 ggccggcgct atgcgttctt cggggaggac caagaggctc tcctggcgcg ggcccgatcc
     3841 ggagaaccag tcaaccttga gctgaaactc caggccgagg acggccgctc cttccccgct
     3901 cacgtgtatc tggcgccgct ccggggggag ggggaggatc tcttggcctg cgtggcggtc
     3961 gcccaggacc tgaccgagga gaaggccaag gagcggcttc aactgcagct cgtccactcg
     4021 gagaagctcg cggccgttgg gcggctggct gcgggagtcg ctcacgagct gaacaacccc
     4081 ctggggaacg ccctgctgta cgcgaagctg ctgctggagg atgcctcccc ctccgagctt
     4141 gggcgcgcga acgcgcggcg catcgtcgag aataccctcc ggtgcaaggc gatcgtgaag
     4201 agcctcctgg attacgccaa gcaatcggag gtccagttga cgtggacgga tctgaacgag
     4261 gttatccagg gctcggtgga cctcgtggcc aacgagctgc gcctccatcg cgtccgctgt
     4321 gaggtccaac tggatcgggg cctgcccaac gtccgctgtg atcggcgcca gatccagcag
     4381 gtcctggtca atctgctcca gaacggcatc gaagccgtcg aaggacaggg caggctgatg
     4441 atcttcagcc ggcgcacgga gaatggggat ggggtcgtgg tgggggtccg ggacgacgga
     4501 ccgggcatcc cgagcgaggc cctctcccgg gtgttcgagc ccttctacac cacgaaggag
     4561 caaggcacgg ggctgggcct cgcgatctgc tacgggattg tggcgcggca tcacgggaga
     4621 atttgggtgg agagttcgga ggacggaggg gcgccgggca gcacattctt cgtggagctg
     4681 ccgctggcgg cggagggtca ggcatgaacg gggatcgcgt gggggagcaa aaccttatcg
     4741 gacggtcggt cctgctcgtg gatgacgagc gggacgccct ggatgcctgt tcccaggccc
     4801 tgggcaagac ccgctacctg gtggagacgg cctccgaccc cctggaggcc ctggagaggg
     4861 tgcgggcccg atcctacgat gcggccgtgc tggacctgaa gctgccgcag atgagcggga
     4921 tcgagctttt acgggccatc cggaagatcg accccgaggt cgcggtgatc ctggtcaccg
     4981 gctacgccac catcgagacg gccgtgcagg ccatgcaaga gggggccagc gactacctcg
     5041 ccaagccctt cacgcccgac gatctcaggc tgggcgtccg gaaggcgatc gagcggcagg
     5101 acctcctccg cgagaaccgg gaactccgag agcgcctggg tcctcggggc gaggcccccg
     5161 ccctgctctg gtcgagccag aagatccagg aggtccgacg actgatcgcg cgcgtgggtg
     5221 cgaccgactc aaccgtcctg atctacggcg agacggggac gggtaaggaa ttggtggctc
     5281 gccagatcca cgccgcaagc cgccgggcca gccagcccct catcgccgtg gattgcgccg
     5341 ccatcccctc cgagctgttg gagagcgaat tcttcggcca cgagaagggc gccttcactg
     5401 gggccatccg gcggcggaag ggaagcttcg aattggccca cggctccacg ctgttcctcg
     5461 acgagatcgg caacatgggt ctggaccttc aggggaagat cttgcgggcc ctccaggagc
     5521 gggaggtcca gccggtcggg agcgagcgga agattccggt ggacgtgcgg attctggccg
     5581 ccaccaaccg gcatctgcgc gaggccatgg agcagggtgg tttccgcgag gatctctact
     5641 atcgtctgaa cgtggtccct ctcttcctgc cgccgctgcg ggaccggaaa gaggatatcc
     5701 ccctgctggt ccagcatttt ctggacaaat acgccagcaa gctgaaccgg cacatccagc
     5761 gggtggagcc cgacgccatg cggatcctgt gtcaatacgc ctggccgggg aacgtgcggg
     5821 agctggaaag cgccatcgaa cgggccatga cattgaccga tggggcggtc ctggaagcgg
     5881 aggcgttccg ccatctcgcc tgggagacag agcgatcccc gaagcctgcg ggctcgaagg
     5941 tgaaacccgg tcgggacgag gcggatgcca atcttccgcc cctgcatcag gtcgagcggg
     6001 actatatcct ggaggtcttg caggccacgc gctggaatcg gaaggaggcg tccaagatcc
     6061 tcggcatcag caccgtcacc ctctggcgca agatcggcgg caagaccacg gacgattcca
     6121 aatgacaacg aatcgtttca ttctgaagtc acgcccgcaa gtgccgaaga ctgcgcgggc
     6181 ggtagccttg gatactgcat tctgaaacga aggcgctcct ccctccccgg gcagctcccc
     6241 catttcgcgc ctcttttcgc ctggcctcca tcttgcttct tctcgcctca aggactccac
     6301 ccatgaacgc tcacatcgca cccccgtgct ccgatccggt cgcgcctggg acccgtcggc
     6361 gtctcatggt ggtcgacgac gccctggaat cgcgtcaggc cctcacggag tatttcgtgg
     6421 cgcgaggcta tgaggtcagc ctggcgaccg atggaatcca ggcgctcgcc aagactctca
     6481 cctccgcggt ggatgtcgtc atcatgaagg ccacgctgcc gggcctcgaa ggatacgagg
     6541 ccgccgccat tctccggaag atcgcccccc gggttcaggt catcctcacc atggaggccg
     6601 gtgtcgaagc ccaacccccg gagcagcgac gcacggagcg cttccgctgc ttccccactc
     6661 ccttgaatct gccggagatt gccggggcgg tcgaaggagc gtgggcgggg accgccccgg
     6721 acctggagcg cgagccgggg gatccgggat gagcggaaga cgaacgctgg cggtgctcct
     6781 catcggcgcg ggtgcgtgca cggcctggct gggtgaccgc cggccggcaa tggccgcctc
     6841 ggctgatccc gaggccggcc gtgctgtctt cgcccgaaag caatgcgttc ggtgccacgc
     6901 gcctcggggg gaggcggggg ccggaccgcc gctggaagag ctgcgccgac cccagggagc
     6961 gatggagatg gcgggccggc tctggaatca tgtgccggcc atgtcggcgg cactcctcca
     7021 gggcgggttg gggtggcccg agatcaacgc cgcggagatg accaacctga tggcctatct
     7081 cctggccgat ccgacgcgag atcccgcacc agaccccgcc aaagggtatg tggcgctctt
     7141 caagaagcaa tgcctgaagt gccatagctt ccggcgagaa ggcggccggg tcgagcccga
     7201 cctggcgaat cggcggccgg actacgagtc cgccgcagcc tgggccgcca ccatgtggac
     7261 gcataccccg cgcatggcgg agatggcgag ccgtcagcag atcctgtttc cgcggttctc
     7321 gggcgatgag atgggcaacc tcctggcctt cctccggggt ggcgccgtga cggcgcctgc
     7381 gaccggacca ggcacggctc cgggccgctg acgcccgatt cccccgcgca caaggagagg
     7441 gtgggtgaag attcctgaac agatccagcg gctggcgctc gtcattgcgg tcctcgttgc
     7501 cggcgtcctg accatccggt tcgtcatcgt cccgcgctcg ctggtggcca tggacctgca
     7561 tc
//
LOCUS       DBNGZC010001049         6455 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_388275, whole genome shotgun sequence.
ACCESSION   DBNGZC010001049 DBNGZC010000000
VERSION     DBNGZC010001049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6455)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6455)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6455
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_388275"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="AC16G0_18065"
     CDS             complement(<1..309)
                     /locus_tag="AC16G0_18065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HIR5101565.1"
                     /translation="MTFLNPAGLLFLLAIPVVIVFHLLKIRRQQVAVSSTLLWADSLR
                     DQQASAPFRRLKPNLLLLLQLLTILVLALTLARPVRSVLASGYERNVFILDVSASMQA
                     R"
     gene            complement(306..1187)
                     /locus_tag="AC16G0_18070"
     CDS             complement(306..1187)
                     /locus_tag="AC16G0_18070"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR5101566.1"
                     /translation="MAGRTFDRDFLQRLEELSIVSRKVSVGRAKGERRSRRKGAGVEF
                     QDYRPYVPGDDLRYVDWSIYCRLERLLLKLFVEEEDLCLHLLVDGSGSMGFGDPPKLD
                     YALQVAAALGYIGLTNLERVALGLFSGDQIRSLRPRRGRGQVFSLLDFLSTAEAGGAT
                     HLNGRLAEYALRSRIPGVAVVLTDLLDPGGYADGLKALLGRRFQVFVVHLVSEEELAP
                     SLRGDLTLLDAEGGAQREVSVDGWALEQYQERLRQHFQEAEGFCAHHHIDYLRTSTAV
                     PFTDLILRHLRRGGFLR"
     gene            complement(1237..2679)
                     /locus_tag="AC16G0_18075"
     CDS             complement(1237..2679)
                     /locus_tag="AC16G0_18075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101567.1"
                     /translation="MNSRELINATLRSLARRLRWVRACAAGVRFLVVGLALALIPLCL
                     KGLFPTAGPVAALALATGLAALGFLYGLLVRLPLSRVARLADQRLHFKERLTSAEERL
                     ALAEPDDVVRAQLTQTVAGLRGVRPRAVFPLRLPREAGWAPPVAALTLALALLPPIPL
                     RLPTPTDEPATQVAKVDEETRENPLEQKPTKPATPNTPFAKGREQKVQRGPLSPRDHQ
                     GDLAAVFKDTKVSEQRPDFGSFVKQGDERLKLLARPDSLPDLSRDFTQSPYQVAIRRM
                     QEQLRSGRLQGLTWDQIERLLSEMGQSQQRLGENGLPDDLMSELNGESGGSPDKMLSA
                     LSRALDRLRDKGGDSQGKGKNLKEAPDSREGQRPGQGEGESDGREDGSAAGSLPGTER
                     SLQTRGESTPRIATQKQDSMLEGDLREGQMEAYNTNLSGGGAQNPSRLPFMDVFSQYK
                     KSMEETLTKEPIPFNYREQVKQYFQSLESR"
     gene            complement(2672..3838)
                     /gene="mnmA"
                     /locus_tag="AC16G0_18080"
     CDS             complement(2672..3838)
                     /gene="mnmA"
                     /locus_tag="AC16G0_18080"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR5101568.1"
                     /translation="MTALAGARDTITVAMSGGVDSAVAAALLVGQGLPTVGITLRIWP
                     SLRPADPLERFDSCCSPGAVEDARGVAETLGIPYYVLNYEEEFDREVIQYFTDSYLRG
                     ETPNPCVSCNSRLKFGSLLRRARGWGATAVATGHYARVTRDQVTGRYLLRRAVDVRKD
                     QSYFLYGLTQEQLGAARFPVGHLRKEETRRLARELGLKVAEKADSQEICFVPRDYRAY
                     LQTRVGSALSPGAIRDTSGAVRGKHPGVAFFTVGQRRGLGVGGSQALYVIDLDPKRNE
                     VIVGEDRDLWAQEVEVAQVNLIAVDRLIAPRRVLAKLRYAQAPAPATLESRGEDRVRL
                     RFDDPQRAVAPGQAAVFYDAEDPDLVVGGGTIRRRGHAGNGSGKGDGLPGSVHE"
     gene            complement(3835..5016)
                     /locus_tag="AC16G0_18085"
     CDS             complement(3835..5016)
                     /locus_tag="AC16G0_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018703150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR5101569.1"
                     /translation="MRMVYLDHNATTPLHPQVLAAMTPYLGERFGNPNSLHAWGREAR
                     QALESSRAAIASALHVADKDTIVFTSGGTEADNLALLGVAAAQQAKGRHIIVSAVEHH
                     AILSPAEFLARQGFELTRLRVDGDGLLDPEEVQRAIRPDTILVSLMHSNNETGVLFPV
                     EQVGRICGEANVTFHTDAVQSLGKLPLDVDALSVDLLSLSAHKIRGPKGVGALYIRRG
                     TKLQPLQRGGAQERSRRAGTENVAGAVGLAKAIELFQPDLDGTAKRLADLRDRLEHGL
                     VGALAGVTRNGHPTERLPQTTNLAFAGVEAESLILALDLQGVGVSSGAACTSGSLEPS
                     HVLTAMGLSRARVGSSLRFSLGTGTTPEEIDYVLQVLPPIVERMRRLNAWTPAQAGVG
                     S"
     gene            complement(5189..5614)
                     /locus_tag="AC16G0_18090"
     CDS             complement(5189..5614)
                     /locus_tag="AC16G0_18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR5101570.1"
                     /translation="MLKRDRERVGADFGEIRAFLGEGTQFKGVLSFAGAVRVDGQLEG
                     EIVGDEVLIIGEPGQVKAEIEVGTVVVSGRVQGTITAKQRVELLRPSRVTGTIRTPCL
                     VVAEGAVFNGNCEMTGPDEGKVVRLEPKQEEGLQAVDRV"
     gene            complement(5828..>6455)
                     /locus_tag="AC16G0_18095"
     CDS             complement(5828..>6455)
                     /locus_tag="AC16G0_18095"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5101571.1"
                     /translation="LYNSVSIALMTTAAVLVVGTLGSFALARIRFRGSRTIQSMTLFT
                     YIIPSSFLSIPFFKLMADYDLIGTQLSVILTMITFASPYAVWILWDYGKTVPQDIDEA
                     AAIDGAGPLALFFRIYLPLIMPPMVAIATYAFLYSWNEYLYAVLFLSGETHITIPVAM
                     GNFLATDNPPWNLLMAESVLYSIPPVILYYCFRRHLLSGLVTGAVSGT"
BASE COUNT         1151 a         2149 c         2067 g         1088 t
ORIGIN      
        1 ccgggcttgc atgctggccg agacgtccag gatgaagacg ttccgctcgt agcccgaggc
       61 caggactgac cgcactggtc tggccagggt cagggcaagc accaggatgg tgaggagctg
      121 gagcagcagc aagaggtttg gcttcagccg gcggaacggc gcgctggcct gctggtcgcg
      181 taggctgtcc gcccacagca gcgtgctact caccgccacc tgctgccgcc ggatcttcag
      241 cagatggaac acgataacca ccgggatcgc caggaggaac agcagccccg ccggattcag
      301 gaatgtcatc ggaggaaccc accccttcgc aggtgtcgca ggatcaagtc ggtaaacgga
      361 accgccgtgc tcgtgcgcag gtaatcaatg tggtggtgcg cgcagaaccc ctccgcctct
      421 tgaaagtgct gcctcagccg ctcctggtat tgctcgaggg cccaaccgtc cacgctaacc
      481 tcccgctgtg cgcctccctc ggcgtccagg agcgtgaggt cccctcgcag gctgggggcc
      541 agttcctcct cgctcaccag atggaccaca aacacctgga agcggcggcc caggagcgcc
      601 tttagcccat cggcatatcc gcccggatcc aggagatcgg tcagcaccac cgccacgccg
      661 ggtatccggc tacgaagcgc gtattccgcc agacgcccgt tcaggtgggt ggcacccccg
      721 gcctcggcgg tggacaggaa gtccagaaga gagaagacct gccctcggcc ccgtcgcggg
      781 cggaggcttc gaatctgatc gccggagaag agacccaggg cgacccgctc caggttggtg
      841 aggccgatat acccaagggc cgccgccacc tggagggcgt agtccagctt cggcgggtcg
      901 ccgaacccca ttgacccgct gccgtccacc aacaggtgga gacacaggtc ctcttcctcc
      961 acgaaaagct tgaggagcag ccgctccagg cggcagtaga tgctccagtc cacataccgg
     1021 aggtcgtccc ccggaacgta ggggcggtaa tcttggaact cgacgcccgc ccccttgcgc
     1081 cggctgcgcc gctcgccttt ggctcgcccc acgctcacct tccgcgagac gatggagagc
     1141 tcctccagcc gttgcaggaa gtctcgatcg aatgtccgcc ctgccatcgc ttcccctctc
     1201 gacccgcccc ggacacggca gccggagacc ctgccctcag cgggattcca gcgactggaa
     1261 gtattgcttc acctgctccc gatagttaaa ggggatcggc tctttggtga gcgtctcctc
     1321 catggatttc ttgtactgcg agaacacatc catgaatggc aaccgcgagg ggttctgggc
     1381 accgccgccg gagagattgg tgttgtacgc ctccatctgg ccctcgcgca ggtccccctc
     1441 cagcatggag tcttgcttct gcgtggcgat ccggggtgtg gattcccccc gcgtctggag
     1501 cgatctctcc gtgccgggca atgaaccggc ggcagacccg tcctcgcgcc cgtcggattc
     1561 gccttccccc tgtcccggcc gttgcccctc acgactgtct ggcgcttcct tcaggttctt
     1621 gcccttcccc tgggagtccc cgcccttgtc gcgaagtcga tccaaggcgc gtgaaagcgc
     1681 ggagagcatt ttgtccgggg agcccccact ttctccattc agctcgctca tcagatcgtc
     1741 cggcagccca ttttcaccga ggcgctgctg gctctgcccc atctcggaga gcagtcgctc
     1801 gatctgatcc caggtgaggc cctggaggcg tccggatcgg agctgctctt gcatccgccg
     1861 gatcgccacc tggtaggggc tctgggtgaa gtcgcggctc aggtcaggca agctatccgg
     1921 ccgcgccaag agcttcagcc gctcatcccc ttgcttcacg aaactgccga agtcaggccg
     1981 ctgttcgctc accttggtat ccttgaagac ggcggcgagg tccccctggt gatcccgggg
     2041 agacaggggg ccgcgctgca ctttctgttc gcgtcccttg gcaaaggggg tgttcggggt
     2101 cgcaggtttc gtaggctttt gctccagcgg gttctcgcgg gtctcctcgt cgacctttgc
     2161 cacctgggtg gccggctcat cggtcggcgt gggcaaccgg aggggaatgg ggggcaagag
     2221 cgccagggcg agggtcagcg cagccaccgg tggtgcccac cccgcctcgc ggggcagtcg
     2281 cagcgggaag acggcgcgcg gtcggacccc ccggagacct gcgactgtct gggtcaattg
     2341 cgcgcggacc acgtcatccg gctcggccag agccagacgc tcttctgcgc tggtcagccg
     2401 ctccttgaag tgcaggcgct ggtcggccag gcgtgccacc cgcgagagag gaagtcgcac
     2461 cagcaggccg tagagaaatc cgagggccgc caggccagtt gccaaggcca gggcggcgac
     2521 cggacccgcg gttgggaaga gccctttcag gcagagcggg atcagggcca gagccaggcc
     2581 taccaccagg aagcggacgc cggcggcgca agcgcggacc cagcgtaggc gacgggcaag
     2641 agaacgaagc gttgcgttga tcagttcgcg actattcatg gacactccct ggaaggccgt
     2701 cccccttccc ggatccgttc ccggcatgtc ctcgtcggcg gatcgtcccg ccccccacca
     2761 cgaggtcggg gtcctctgcg tcgtagaaaa ccgccgcctg ccccggggcg acagcccgct
     2821 gggggtcatc gaagcgcaag cggacccggt cctcaccccg cgactccagc gtggccgggg
     2881 ctggggcctg ggcatagcga agcttcgcca agacccgccg tggggcgatc agacgatcca
     2941 ccgcgatgag gttcacctgt gccacctcga cctcctgcgc ccagaggtcg cgatcctccc
     3001 ccacgatcac ttcgtttcgc ttggggtcga ggtcgatcac gtacaaagcc tggcttcctc
     3061 ccaccccaag accgcgtcgc tgtcccacgg tgaagaacgc cacgcccgga tgcttgccgc
     3121 ggacggcccc gctggtgtcc cggatggctc ccggcgatag ggcagagccg acccgcgtct
     3181 gcaggtaggc gcgatagtct cgcgggacga agcagatctc ctggctgtcc gctttctccg
     3241 ccaccttcaa gcccagctca cgggcgaggc gacgcgtttc ctccttacgc aggtggccga
     3301 ccgggaaccg ggcagccccc aactgctcct gggtgaggcc gtagaggaaa tagctctggt
     3361 ccttccggac atccaccgct ctgcggagga ggtagcggcc cgtcacctga tcgcgcgtca
     3421 cccgggcgta atggcccgtc gccaccgcag ttgcgcccca gccccgcgcc cgcctcagga
     3481 gcgagccgaa cttgagccgg gagttgcagg acacgcaggg gttgggtgtt tccccgcgga
     3541 ggtaagaatc ggtgaaatac tggatgacct cgcggtcgaa ctcctcctcg tagttcaaga
     3601 cataatacgg gatccccaaa gtctcggcca cgcccctggc gtcctccacc gcccccggcg
     3661 aacagcaact gtcaaaccgc tccagcgggt ccgccgggcg cagactgggc cagatccgga
     3721 gggtgatccc gaccgtgggt aggccctgcc ccaccaggag cgccgccgcc acggcggaat
     3781 cgaccccgcc gctcatggcc acggtgatcg tgtcgcgtgc tcctgcgagg gctgtcacga
     3841 ccccacccct gcctgcgcgg gcgtccaagc gttcagacgg cgcatccgct cgacaatcgg
     3901 ggggaggact tgcagcacgt agtcgatctc ctccggtgtg gtccccgttc ccagagagaa
     3961 tcggagcgag ctccccaccc gcgcccgcga aagccccatg gcggtcagga cgtgtgaggg
     4021 ttccagcgag cccgaggtgc atgcggcgcc cgaggaaact cccacaccct ggaggtccag
     4081 ggcaaggatc aaggactccg cttccactcc ggcgaaagcc aggttagttg tttgtggcaa
     4141 tcgctcggtc ggatgaccgt ttcgtgtcac cccggccagg gcgccaacca gcccgtgctc
     4201 taaccggtcc cgcaggtccg ccagccgctt ggctgtcccg tccagatccg gttggaacag
     4261 ctcgatggcc ttggcgaggc cgaccgctcc ggccacgttc tccgtgcccg cacggcgcga
     4321 gcgttcctgg gcccccccac gttgcagggg ttgcagtttc gtcccgcgac ggatgtagag
     4381 ggcccccacg cccttcggcc cgcgaatttt atgggccgag agagacagga gatccaccga
     4441 caaagcgtcc acatccaggg ggagcttccc caggctctgg acggcatcgg tgtggaatgt
     4501 gacgttggct tcgccacaga tccgcccaac ctgttcgacg gggaacagga cgccggtctc
     4561 gttgttgcta tgcatgaggc tgaccaggat ggtgtccggc cggatggcgc gctgtacctc
     4621 ctcagggtcc aggagcccgt cgccatctac gcggagacgc gtcagttcaa agccctgccg
     4681 tgcgaggaat tccgcgggag ataggatggc atggtgctcg acggccgaga caatgatgtg
     4741 tcggcccttg gcctgctgcg ccgcggccac acccaggagg gccaggttgt cagcctccgt
     4801 ccctccggag gtgaacacga tggtatcctt gtccgcgacg tgcagggcac tcgcgatggc
     4861 agcccgcgag gactcgagag cctggcgggc ctcgcggccc caggcgtgga gggagttggg
     4921 gttcccgaac cgctccccga ggtaaggcgt catggccgcc aacacttggg ggtgaagcgg
     4981 ggtagtagca ttgtgatcga gataaaccat ccgcatgcca cgttccccag actggaggtg
     5041 gagcccaaca cagcctcaga ccaggacaag gctatcgcac gcaggaggag cggacggcag
     5101 gcggaacgca ccggggaatg cgagtgacga cgatgccgga agccagagtg cgtaagtggt
     5161 ccggaacctg gaaggcaggg gcgtgggctt acacccggtc aaccgcctgc aggccctcct
     5221 cctgtttcgg ctccaggcgc acgaccttgc cttcatccgg gccggtcatc tcacagttcc
     5281 cgttgaacac ggcgccttcg gccaccacca aacagggggt gcggatcgtg ccggtcacgc
     5341 gactcggtcg caggagctcc acgcgctgct tcgcggtgat tgtcccctgg acccgaccgc
     5401 tcacgaccac tgtcccgacc tcgatctcgg ctttcacctg gccgggctcg ccgattatga
     5461 gcacctcatc gccaacgatc tctccctcga gttgaccgtc aacgcggacg gctccagcga
     5521 aactcaacac ccccttgaac tgggttccct ccccaaggaa ggctcggatc tcgccgaagt
     5581 cggcgccgac ccgctccctg tcccgcttga gcacggtgac ctcctagggt aatgaggacg
     5641 actcttcatc cgcacagaat aaaagtcttc ggtggacatt tacgctgaga atattaatga
     5701 cgaccctaat ggtgacgaat ggtgtcacgg tagcagagca tttctgggct gtcaattcga
     5761 aagtcgccga tgtttcccct gctgacgagc gcaactcggt tacgcttcgc cgttaggaag
     5821 catcgagcta cgtcccggac accgcccccg tcacaagccc gctcaggaga tggcgccgga
     5881 agcaataata gaggatgacg ggcggaatgg agtagaggac cgactcggcc atcagaaggt
     5941 tccagggtgg gttgtccgtg gccaggaagt tgcccatggc cacggggatc gtgatgtggg
     6001 tctcgcctga gaggaacaga acggcgtaca ggtactcgtt ccacgaatac aaaaaggcat
     6061 aggtcgcgat ggcaaccatg ggcggcatga tgagcgggag gtagatccgg aagaatagag
     6121 ccaacggccc tgcgccgtcg atggccgccg cctcatcgat gtcctgaggg acagtcttcc
     6181 cgtagtccca caggatccag acagcatagg gggacgcgaa cgtgatcatg gttagaatca
     6241 ccgagagttg cgttccgatc agatcatagt ccgccatgag cttgaagaag gggatggaca
     6301 agaaggacga ggggatgatg taggtgaaga gcgtcatgct ctggatggtc cggctccccc
     6361 gaaagcggat ccgggcgagg gcgaagctgc ccaaggtgcc aaccacgagg accgccgccg
     6421 tggtcatgag ggcgatgctc acgctgttgt aaagc
//
LOCUS       DBNGZC010001050         3309 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_388277, whole genome shotgun sequence.
ACCESSION   DBNGZC010001050 DBNGZC010000000
VERSION     DBNGZC010001050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_388277"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1369)
                     /locus_tag="AC16G0_18100"
     CDS             complement(<1..1369)
                     /locus_tag="AC16G0_18100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011733967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HIR5101572.1"
                     /translation="MTTALLSAALVLLAAILVLALLLLRKVSQGDARALTPRLDAFEK
                     AQERTERAVREEVAQSRDESGKAAREQRQELTGAFKTFGDSVVQRMMDVAIMQKAQLD
                     AFSDQLASFAKASGERLDAVRAESATGARQLREEVLVTLKGLSDTMTKTMGELANSQM
                     TQLEAMATQLGKLSESNEKKLESVRTTVESKLQSLQSDNAAKLEQMRATVEEKLQGTL
                     DKRLGESFKQVSERLEQVYKGLGEMQSLATGVGDLKKVLTNVKTRGTWGEVQLGAMLE
                     QVLNPDQYAANVVTKDGGGPVEFAVKLPGRGDDQDELVWLPIDAKFPIEDYQRLVDAQ
                     EKGEADAAEAAAKQLENRVKACARDICEKYLNPPRTTDFGVLFLPIEGLFAEVVRRAG
                     LADLVQREHRVMIAGPTTLWSILNSLQMGFRTLAIQKRSSEVWNLLAAVKAEWSKYGE
                     ILDKVH"
     gene            complement(1576..2835)
                     /locus_tag="AC16G0_18105"
     CDS             complement(1576..2835)
                     /locus_tag="AC16G0_18105"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5101573.1"
                     /translation="MSIHGGRDHGMDRRTFLEIGAGAMGGLLVGLGPTRAWGADVKVA
                     GLLALTGTNAAWGQKTANSFQLAWDIINQQGGVKALGGAKVAYSVADTESRPEVAGSQ
                     TERLIQQANIAVVGCNQSAASVVASQVSERLQVPFMTPTDVDPLVTSRGFKYSFRLPP
                     LVDAYAKDLLTYAVEMGKATGKPAKKLAILCENSIIGQSAAKASAQFGKELGFEVMDV
                     STYDAATQPDFTGYISKYKSGGVQILIGHNKPTDAVLITRTLKELDLNLIMYGGLLGG
                     HESVEYIRSLGADSDYVTFVTAWAPDLKVPGMDELAKQYTAKYKVDTDAYFAAGFSAA
                     SVLWDALERARSTDRKALRDAIAATEMKTGDRFYLMLHGCKFQPNGDNARAGAVVEQI
                     RDGKWHTVAPAQFAAAKPVWPKPNWRR"
     gene            complement(2889..>3309)
                     /locus_tag="AC16G0_18110"
     CDS             complement(2889..>3309)
                     /locus_tag="AC16G0_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5101574.1"
                     /translation="NLELGAFTPKARGRVRQSMEEVFALFPRLRERQDQVVETLSGGE
                     QQMVAIGRALMAVPRLLMLDEPSLGLDPLQVRSIFRTITRVNSEGRSILLVEQNLGMA
                     LRISHRAYVLETGRVVLEGTGESLLRDERTKRAFLGM"
BASE COUNT          498 a         1146 c         1012 g          653 t
ORIGIN      
        1 tgtggacctt gtccaggatc tccccgtact tgctccattc tgccttgacc gcagccagga
       61 gattccagac ctcgctggat cgcttctgga tagccagcgt tcggaagccc atctgcaggc
      121 tgttcaagat ggaccagagc gtggtggggc cggcgatcat cacccggtgc tctctctgaa
      181 cgaggtcggc caggcccgct cgacgcacga cttcggcaaa gaggccttcg atgggaagga
      241 acaggacgcc aaagtccgtg gtcctcggcg gattcaagta cttttcacag atgtcccgcg
      301 cgcacgcctt gacccggttt tcaagctgct tggccgcggc ctcggcggca tcagcctcgc
      361 ccttctcctg ggcatccacc agtcgctgat agtcctcgat ggggaacttg gcgtcgatgg
      421 ggagccacac gagttcgtct tggtcatccc cacgccccgg cagcttgacg gcaaactcaa
      481 ccgggccgcc accgtccttc gtcaccacgt ttgcagcgta ctgatctgga ttgaggacct
      541 gctccagcat ggcgccgagc tggacctcgc cccaggtgcc acgggtcttg acgtttgtca
      601 gcaccttctt gagatcgccc acgccggtgg cgaggctctg catctcgccg aggcccttgt
      661 acacctgctc cagtcgctcg ctgacctgct tgaacgattc gcccaggcgc ttatccagcg
      721 tcccctgcag cttctcctcc acggtcgcgc gcatctgctc cagcttggcg gcgttgtcgc
      781 tctggagact ttggagcttg gactccacgg tcgtcctgac cgattccagc ttcttctcgt
      841 tggattccga cagtttcccc aactgcgtgg ccatggcttc cagctgggtc atctgggagt
      901 tggccaattc gcccatcgtc ttggtcatgg tatcagacaa ccccttcagg gtgacgagga
      961 cttcctctcg caattgcctg gcgcctgtgg ccgactcggc gcgcacagcg tcgagtctct
     1021 ccccgctggc cttggcgaag gaggccagtt gatcggagaa ggcgtcaagc tgcgccttct
     1081 gcataatcgc cacatccatc atgcgttgca ccacggagtc gccgaaggtc ttgaacgcgc
     1141 cagtgagttc ctgccgctgt tctcttgccg cctttcccga ttcgtctctg ctctgggcca
     1201 cctcctctct gaccgcacgc tccgtcctct cttgggcctt ctcgaaggca tccaggcgtg
     1261 gagtgagagc cctggcatcg ccctgcgaca ccttcctcag gagcagcaag gcgaggacca
     1321 ggatggcggc cagcaggaca agagccgcac tgagcagcgc agtggtcatg cgttccctct
     1381 ccgtgatggc gggcaggatg cgattctacc cgccccaaag atcggggagt ctccggttcc
     1441 gctctcgccc ccgtgtggcc ccggtggcgt gggccacacg ggggctcacc ctctacgggt
     1501 ggatttcgga cacagtcccc tagggggctt tccggcgcac gcgttggggc ggtcacacct
     1561 ccaggcgaac agggatcacc gtcgccagtt cggcttgggc cacacgggct tggctgccgc
     1621 gaactgcgcc ggggcaaccg tgtgccactt gccatcccgg atctgctcga ccacggctcc
     1681 ggccctggcg ttgtccccgt tcggctggaa cttgcagccg tgcagcatga ggtagaaacg
     1741 gtcgccggtc ttcatttcag tcgccgcgat ggcgtcccgg agggcctttc gatctgtgct
     1801 gcgggctcgc tccaaggcgt cccacagcac cgaggcggcg gagaaccccg ccgcaaagta
     1861 ggcgtcggtg tccaccttgt acttggcggt gtattgcttg gccagctcat ccatccccgg
     1921 gaccttcagg tccggcgccc aggcggtgac gaaggtcacg tagtcggaat ccgcgcccag
     1981 ggatcggatg tactccacgc tctcgtgccc gcccaggaga cctccataca tgatgaggtt
     2041 caggtccagc tccttcaggg tgcgggtgat gagcacggcg tccgtgggct tgttgtgccc
     2101 gatcaggatc tgcacgccgc cgctcttgta cttggagatg taccccgtga agtccggctg
     2161 ggtcgcggcg tcgtaggttg acacgtccat cacctcgaag cccagctcct tgccgaattg
     2221 cgccgaggcc ttggcggcgc tctggccgat gatggagttc tcgcagagaa tggccagctt
     2281 cttggccggc ttgccggtcg cctttcccat ctcgacggca taggtcagca ggtccttggc
     2341 gtaggcatcg accaggggcg gcagccggaa cgagtacttg aatcctcgcg aggttacgag
     2401 cgggtccacg tccgtggggg tcatgaaggg gacctgcagg cgttcgctga cctggctcgc
     2461 caccacgctg gcggcgctct ggttgcagcc caccaccgcg atgtttgcct gctggatcag
     2521 ccgctccgtc tgcgagcccg cgacctccgg ccggctctcc gtgtcggcca cggagtaggc
     2581 taccttggcc ccgcccaggg ccttcacccc accctgctgg ttgatgatgt cccaggcgag
     2641 ctggaagctg ttggccgtct tctgtcccca ggcggcgttg gtccccgtga gggcgagcag
     2701 cccggcgact ttcacgtccg ccccccacgc ccgggtcggc cccaggccca ccaagaggcc
     2761 tcccatcgct ccagcgccga tctccaggaa cgtccgccgg tccatgccat ggtcgcgtcc
     2821 gccgtgtatg ctcatcgcca tccctccttg gcccgctcac ggccgctgag ccgtaaatgg
     2881 gccacgcctc acatccccag gaacgcccgc ttggtccgct cgtcgcgcag gaggctctcc
     2941 cccgtgcctt ccagaaccac ccgcccggtc tcgaggacgt aggcgcggtg ggagatccgg
     3001 agcgccatcc ccaggttctg ctccaccagc aggatggatc gcccttcgct gttgacccgc
     3061 gtgatggtgc ggaagatgct ccgcacctga agaggatcga ggcccagcga aggctcgtcc
     3121 agcatcaaca ggcgaggcac ggccatcaag gcccggccga tggccaccat ctgctgctcg
     3181 ccgcccgaga gggtttccac cacctggtcc tggcgctccc ggaggcgggg gaagagggcg
     3241 aagacctctt ccatgctctg ccgcaccctc ccgcgggcct tgggcgtgaa ggcccccagc
     3301 tccaggttc
//
LOCUS       DBNGZC010001051         2212 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1655029, whole genome shotgun sequence.
ACCESSION   DBNGZC010001051 DBNGZC010000000
VERSION     DBNGZC010001051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1655029"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="AC16G0_18115"
     CDS             <1..353
                     /locus_tag="AC16G0_18115"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR5101575.1"
                     /translation="GELLTQQKARGVGRLYCTFAATCRSNDSGNGPLPCATRDLSSSG
                     VALRLPAPVSPGTPLQVTVLTPFGVISADTQVVWAEPRDGLPRGAPCRHGLRFLRLES
                     SSDLPLKVLLAGLR"
     gene            920..1441
                     /locus_tag="AC16G0_18120"
     CDS             920..1441
                     /locus_tag="AC16G0_18120"
                     /inference="COORDINATES: protein motif:HMM:NF013629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR5101576.1"
                     /translation="MASPARLKQFEAACRAHGLPVTVQRRCIFEALCDRTDHPTPDHV
                     YALVKDILPGISRTTVYRVLETLVRVGVLTKACSPSAAGRVDPRTSRHHHLVCQHCNR
                     LFDVDEDAVKYRIRPPDVRRQGFAIQGYSIYFTGLCAACQNPAVTQPTRRAGLAPVRK
                     ASRPRPTTIRARG"
     gene            1797..>2212
                     /locus_tag="AC16G0_18125"
     CDS             1797..>2212
                     /locus_tag="AC16G0_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HIR5101577.1"
                     /translation="MSTETKCPFPHTAGGGTSNRDWWPNQLRLDILHQQSSKSNPMGK
                     DFNYPKEFKSLDLAAVKKDLAALMTDSQDWWPADFGHYGPLFIRMAWHSAGTYRIGDG
                     RGGGGRGQQRFAPINSWPDNVSLDKARRLLWPIKQK"
BASE COUNT          395 a          770 c          648 g          399 t
ORIGIN      
        1 tgggggagct gctcacccag cagaaagccc gcggcgtcgg gcgcctctat tgtaccttcg
       61 ccgccacctg ccggagcaat gactccggca atggcccgct cccctgcgcg acccgggatc
      121 tctcctccag tggggtcgcc ctccgcctgc cggcgccggt gtcgccgggc acgcccctcc
      181 aggtgacggt cctcacgccc tttggcgtca tctcggccga cacccaagtc gtctgggccg
      241 agccccgcga cgggctcccc cggggtgccc cctgccgtca cggcctgcgg tttctgcgtc
      301 tcgagtcgtc gagcgatctg cccctgaaag tgctgctggc tggactgcgg taggcaaagg
      361 caatcatcgt gggagccgga agccccgtgg ggctggctcg acacggcccg gagtgcattc
      421 gcgacagtga gtgactccct tctgggggcg tgggatccga cctccggcct gaaaatcatc
      481 cggatgcccg agccgagagg gaaaggggct ccccggcgga actctctggg ttctgttgcc
      541 gacttgtgct ccctacggcg gtgcaattcg ccccatggct caagggttgt tggttaaaat
      601 ccagccctcg atttggatca atgaatctga aacgcttctt cccaataggg tagctccccc
      661 cagtctggtt cgtgcgcaga ctgtagggaa caggccctcg gcaccctaac ggagcgaacc
      721 ggcccatcac tccaagccgt ccgtctggtt tggtttgcca tcgttattcg atactcggcc
      781 cgcgctccct atcccacaag atgtccactg gcctttatct ccgagactcc ttgacaagtt
      841 tctctagatg gtacaatgta ccatctgttc tgtttgaccc ggccttgtcc tctctgtcgt
      901 ccaccgaccg gagacgcctg tggcgagtcc cgcacgcctc aagcagttcg aagccgcctg
      961 ccgggcccat ggcctgccgg tcaccgtgca acgccgctgc atctttgaag ccctatgcga
     1021 ccgcaccgac caccccaccc ccgatcacgt gtacgccctg gtgaaggata ttttaccggg
     1081 tatttcgcgc acgacggtgt atcgggtgct cgagacgctc gtccgggtgg gggttcttac
     1141 gaaagcgtgc agccctagtg ctgcgggtcg tgtggacccc cggacgtccc ggcaccatca
     1201 tctcgtctgc cagcactgca accgcctctt cgatgtggac gaggacgccg tcaagtaccg
     1261 gattcggccg cccgatgtcc ggcgccaggg cttcgccatc caggggtact cgatctactt
     1321 caccggcctg tgtgccgcat gccaaaatcc ggcggtaacc caaccgactc ggcgagctgg
     1381 gctggcaccg gtccggaaag cctcgcgccc aagaccgaca accatacgcg cccggggcta
     1441 gccctggacg caagtccccg caagcccgca gcgccccaga aaggagccgc ctcatgacac
     1501 accagccgaa gaaattgacc agcatcagcg gcgcccctgt ccccgataac cggaacgtca
     1561 tgaccgctgg cccgcgcagc ccactccttg tgcgggattg gcaactgttc cttccttgac
     1621 agtcttgtcc catccggcaa gacaccgcca ccccggtcca gccggaatcg ggcctcctgg
     1681 cccggcgcgg gagggctccc tgcgtggcga agcggatcgt cgcgcgacaa caaagggcag
     1741 gttcctatca ccaactggcc gagtaattga gtgcaagcaa ccaaaggaga gaacccatgt
     1801 caaccgaaac caagtgcccg ttccctcaca ccgctggcgg cggcacgtcg aaccgcgact
     1861 ggtggcccaa ccagttgcgc ctggacatcc tgcaccagca gtcctccaaa tccaatccga
     1921 tgggcaagga cttcaattac ccgaaagagt tcaagagcct cgacctcgct gctgtgaaga
     1981 aagacctcgc cgcgctgatg accgactctc aggattggtg gccggcggac ttcggtcact
     2041 acggaccctt gttcattcgc atggcgtggc acagcgccgg cacgtaccgc atcggcgacg
     2101 gccgcggcgg cggcggcagg ggccagcagc gctttgcgcc gatcaatagc tggccggaca
     2161 acgtcagcct ggacaaggcg cgtcgtctgc tctggccgat caagcagaag ta
//
LOCUS       DBNGZC010001052         2685 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2533549, whole genome shotgun sequence.
ACCESSION   DBNGZC010001052 DBNGZC010000000
VERSION     DBNGZC010001052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2533549"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1751
                     /locus_tag="AC16G0_18130"
     CDS             <1..1751
                     /locus_tag="AC16G0_18130"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HIR5101578.1"
                     /translation="KPGVDPLGPDNVMVFATGPCNGSPISGTNRFACATKSPLTGGYG
                     EGEAGGWWGPELKFAGFDGIIITGRSPKPVYLWIADGKAELRDGAHLWGKISGQVQES
                     LTAETDKRARVLQCGIAGEKGIRLANMVNELKHFIGRSGLGAVMGSKRLRAIAVRGHS
                     KLEAKDPQGVQAVLKWFREHYNRDADMLHKFGTSRNIKGMNADGILPSYNFQKGQFEH
                     WEGITGQHMAETILEDEGTCFACAVACKREVNVPELGVSSEYGGPEYETIAASGSACG
                     VGDIKAIAKFHQICGEYVMDTISVGMTIAFAMECFENGILTTADTDGIELRFGNADAL
                     IKVTEKMAKREGFGDVLADGAKRAAERIGRGAERFALTVKGQEIPMHEPRGKQGLSLA
                     YATTASGADHMRAPHDPIYEGFHPEGAHALEAMGLCEPLPRLELSPRKVRAFYYANNW
                     WSLCSSAGICQLAAVPIDILAITQVVNLVRALTGWDTSLWELVKVAERGKALARVINC
                     REGFTAKDDTLPKRLQEAFTDGPLKDVRIDPDKFSRALRLYQQMEGWDTETGWPTFSK
                     LAELGIEWAAKPGETW"
     gene            1807..2601
                     /locus_tag="AC16G0_18135"
     CDS             1807..2601
                     /locus_tag="AC16G0_18135"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5101579.1"
                     /translation="MRLEGKVAIVTGGAQGIGRGIVLCMAKEVADVAIADLKVEEAAK
                     VADEVKALGRKAMVVKTDVTKQAEFEALFERVKKELGKIDILVNNAGTASPPGNPFTN
                     NVEKDWDVVWAVNVKSIFFACKAIAPYFIQRKTGKIINIASIAGTMNSPNMPPYSVSK
                     MGVVTFTKIVAKELATHGINVNAICPGILWTAFWQETAERMAKGGGQYAGMTPRQVFE
                     SRVNAVIPMKREQTPEDIGNAAVFLASEDARNVTGQALHVDGGVVM"
BASE COUNT          520 a          816 c          941 g          408 t
ORIGIN      
        1 tgaagccggg ggtagacccc ctgggcccgg ataacgtcat ggtcttcgcc accggtccgt
       61 gcaacgggag tcccatctcc ggcaccaacc gcttcgcctg cgcgaccaag tctcccctca
      121 cgggcgggta tggtgaaggc gaggccggtg gctggtgggg gccggagctg aagttcgccg
      181 gattcgacgg catcatcatc accggccggt ctccaaagcc ggtgtacctg tggatcgcag
      241 acggcaaggc cgagctgcgg gacggcgccc atctctgggg caagatctcc ggccaggttc
      301 aggagagcct gacagccgag acggacaagc gggcccgcgt cctccagtgc gggattgccg
      361 gcgagaaggg catccgcctg gccaacatgg tgaacgagct gaagcacttc atcggccgga
      421 gcggtctcgg cgcggtcatg ggaagcaagc ggctccgggc catcgcggtc cgcggccact
      481 ccaagctgga ggccaaggac ccgcagggcg tccaggccgt cctgaagtgg ttccgcgagc
      541 attacaaccg cgacgccgac atgctccaca agttcggcac ctcgcggaac atcaaaggca
      601 tgaatgccga cgggattctg ccaagctaca actttcagaa gggccagttc gagcactggg
      661 aggggatcac cggccagcac atggccgaga ccatcctgga ggacgagggg acctgttttg
      721 cttgcgccgt cgcctgcaag cgcgaggtca acgtgcccga gctgggtgtc tcctcggaat
      781 acggcgggcc ggagtacgag acgatcgcgg ccagcggctc ggcctgcggg gtgggggata
      841 tcaaagccat cgccaagttc caccagatct gtggcgagta cgtcatggac accatctccg
      901 tggggatgac catcgctttc gccatggagt gctttgagaa tggcatcctg accacggccg
      961 acaccgacgg gatcgagctg cgcttcggca acgccgatgc tctgatcaag gtgaccgaga
     1021 agatggcgaa gcgcgagggc ttcggcgacg tcctggcgga cggggccaag cgggccgccg
     1081 agcggatcgg ccgcggggcg gagcggttcg ccctcacggt caagggtcag gagattccca
     1141 tgcacgagcc gcggggcaag cagggcctgt ccctggccta tgccacgaca gcctccggag
     1201 ccgaccacat gcgggccccg cacgacccga tatacgaggg cttccacccc gagggggccc
     1261 acgccctgga agccatgggt ctgtgcgagc cgctgcccag actggagctg tcgccacgca
     1321 aagtgcgggc gttctattat gccaacaact ggtggagcct gtgcagctcg gcggggatct
     1381 gtcagctggc ggcggtcccc atagatatcc tggcgatcac ccaggtggtg aatctggtgc
     1441 gggccctcac cggctgggat accagcctgt gggagctggt caaggtggcc gagcgtggga
     1501 aggcgctggc gcgggtcatc aactgccgcg agggcttcac ggccaaggac gatacgttgc
     1561 cgaagcgtct ccaggaggcc ttcactgacg gcccgttgaa ggacgtccgg attgacccag
     1621 acaagttcag ccgggcgctc cgcctgtatc agcagatgga gggctgggac acggagaccg
     1681 gctggccgac gttctccaag ctggccgagc tgggtatcga gtgggccgcg aagccggggg
     1741 agacctggta agtcgcagtc ccggcagcag aagagtggat ccccacacct gagaaaggac
     1801 ttcagcatgc gactggaagg aaaggtggcc atcgtaacag gaggcgccca gggtatcggg
     1861 cgtggtatcg tgctgtgcat ggccaaggaa gtggcggacg tcgccattgc cgatctgaag
     1921 gtcgaagaag ccgccaaggt ggcggacgag gtcaaggccc tgggccggaa ggccatggtg
     1981 gtcaagaccg acgtgaccaa gcaggcggag ttcgaggcgc tcttcgaaag ggtgaagaag
     2041 gagctgggga agatcgacat cctggtgaac aacgcgggga cggccagccc gcccggcaat
     2101 cccttcacca acaacgtcga gaaggactgg gacgtggtct gggcggtcaa cgtgaagtcc
     2161 atcttcttcg cctgcaaggc catcgccccc tacttcatcc agcggaagac ggggaagatc
     2221 atcaacatcg cctccatcgc ggggaccatg aactcgccga acatgccccc ctacagcgtg
     2281 tccaagatgg gggtggtcac gttcaccaag atcgtggcca aggagctggc gactcacggg
     2341 atcaacgtca acgccatctg ccccggcatc ctctggaccg cgttctggca ggagacggcc
     2401 gagcggatgg cgaagggcgg cgggcagtat gccgggatga caccgcgcca ggttttcgag
     2461 tcgcgggtga atgccgtcat ccccatgaag cgcgagcaga cgcccgaaga catcgggaac
     2521 gcagcggtgt tcctcgcatc cgaggacgcc cgcaacgtca ccgggcaggc cctccacgtg
     2581 gacggcggcg tggtgatgta gcggggcgga tcagggggtg ggctgtcttt catcgagcgg
     2641 ggtgccggat atgccgggac cccgctcgtg cgtcccggcc atccg
//
LOCUS       DBNGZC010001053         2084 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1361985, whole genome shotgun sequence.
ACCESSION   DBNGZC010001053 DBNGZC010000000
VERSION     DBNGZC010001053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1361985"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="AC16G0_18140"
     CDS             <1..274
                     /locus_tag="AC16G0_18140"
                     /inference="COORDINATES: protein motif:HMM:NF013481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HIR5101580.1"
                     /translation="GERAYNLARAFTVRESGGQADDRLPAKLSRRLQDGASRDQVITH
                     QDLQPVLQEYYRLRGWSESGTPTEVKLKELGLADVAQQLAEKGDGR"
     gene            271..738
                     /locus_tag="AC16G0_18145"
     CDS             271..738
                     /locus_tag="AC16G0_18145"
                     /inference="COORDINATES: protein motif:HMM:NF024644.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5101581.1"
                     /translation="MSRMIVVDPAKCTNCRMCELACSLITTGAFAPSRSRIRVHAFAA
                     EFKYIPLACFQCAAAPCVKVCPAGALVQGDGLVRFVKEACIGCRMCMLGCPFGVIAFD
                     SAEGVVAKCDTCNGDPECVRFCTPHALEFRESELADVPKGRDFARRVAEAAKG"
     gene            781..2055
                     /locus_tag="AC16G0_18150"
     CDS             781..2055
                     /locus_tag="AC16G0_18150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5101582.1"
                     /translation="MSQRRIVIVGNSAASLAALESIRRLDGTSPITLIADEAIPAYSR
                     VLLPYLVSGERRDLSLRPPDYARRMGARMLLGHRAVQVDTDALVLADGTRLPFDRLLL
                     ATGSRAAIPDIEGIDTPGVSPLKTMTDALRVREQLARARHAVILGGGLICLLVVRALL
                     KMGLSVAIAVSSDRLLTRMLDEEAAALLQNRLAEAGVRILTRTDAARIVGNEGRVRSV
                     VTAVGEVLPADMVILAKGIRPDIDLARAGGLATDRGVLVDEYLRTSRPSVFAAGDCAE
                     GSDMLVPGKKTIPGTWFEAVAQGECAGANMLDLASPSPGAFKMNVMEILGVAVASMGL
                     IQAPDSDGRVVVRARNGNYRKLVISRDRIVGAVLVGDVSEASAIAMLIRRRLTLSELR
                     PFDLSQPICYADLAMQCGSRTFDSVGCRPHWS"
BASE COUNT          322 a          669 c          742 g          351 t
ORIGIN      
        1 cggcgagcgg gcctacaacc tggcacgcgc attcacggtg cgggagagcg gcggccaggc
       61 cgacgaccgg ctcccggcca agctgtcccg gcggctgcag gacggggcat cccgggacca
      121 ggtcatcact caccaggacc tccagcccgt gctgcaagaa tattaccggt tgcgcggctg
      181 gagcgagagc ggcacaccca ccgaggtgaa actcaaggaa ttgggcctgg cggacgtggc
      241 ccagcagctt gcggaaaaag gagacggccg atgagtcgca tgatcgtcgt ggatcctgcc
      301 aagtgcacca attgccggat gtgtgagctg gcctgctcgc ttatcaccac tggcgccttt
      361 gccccctcgc ggagccgcat ccgggtccac gcctttgccg cggagttcaa gtatatcccc
      421 ctggcctgct tccagtgtgc ggcggcgccg tgcgtcaagg tctgcccggc cggcgccctc
      481 gtgcaggggg acggactggt gcgctttgtg aaggaggcct gcatcggctg ccgcatgtgc
      541 atgctcggct gcccgttcgg ggtgatcgcg ttcgatagcg cggagggggt agtcgccaag
      601 tgcgacacct gcaacggcga tcccgagtgc gtgaggtttt gcacgccgca tgctctggag
      661 ttccgggagt cggaactggc ggacgtgccc aaggggcgag acttcgctcg tcgagtcgcc
      721 gaggcagcca agggctagcg gcccggccca ccggcgggat cgccggtgca aggagaacag
      781 gtgtcgcaac ggcgaatcgt catcgtcggc aacagcgccg ccagcctggc tgccctggag
      841 agcatccggc gcctggacgg cacatccccc atcaccctga tcgcggatga ggcgatcccc
      901 gcctattccc gggtgttgct gccctacctg gtgagcggag agcgtcgcga cctgtcgctc
      961 cgaccgcccg actacgcccg gcggatgggc gcgcggatgc tgcttggcca cagggctgtg
     1021 caggtcgaca ccgatgcgct ggtgctggcc gacgggaccc gcctgccctt cgatcggctc
     1081 ctgctggcca ccggatcccg ggccgcgatc ccggacatcg aagggatcga cacgccgggg
     1141 gtctctcccc tcaagacgat gaccgacgcg ctgcgggtcc gggaacagtt ggcgagggcg
     1201 cgccatgcgg tcattctcgg aggtgggctc atctgcctcc tggtggtccg cgcgctgctc
     1261 aagatgggtc tctcggtggc gatcgcggtc tcgtcggatc ggctcctcac gcgcatgctg
     1321 gacgaggagg ccgcggccct tctccagaat cgcctggcgg aagccggggt ccgtatcctc
     1381 acacgcaccg acgccgcgcg cattgttggc aacgagggta gggtccgttc cgtggtgacg
     1441 gcggtcggcg aggtcctccc ggcggacatg gtgattctgg cgaaagggat ccggccggat
     1501 atcgacctgg cccgggccgg cgggctggcc accgaccgcg gtgtcctggt cgacgagtac
     1561 ctgcgcacga gccgcccaag cgttttcgcg gcaggcgatt gcgccgaggg atccgatatg
     1621 ctcgtgcccg ggaagaagac gattcccggg acgtggttcg aggccgtggc ccagggcgaa
     1681 tgcgcagggg ccaacatgct ggatctggcg agtcccagtc cgggggcctt caagatgaac
     1741 gtgatggaga tcctgggagt cgctgtggcc tccatggggc tgatacaagc gccggactcg
     1801 gatgggcggg tcgtggtgcg tgcgcgaaat ggcaactatc ggaagctcgt gatctcacga
     1861 gaccggatcg tgggggctgt gctcgtgggc gatgtgtccg aggcgagcgc gatcgcgatg
     1921 ctgatccgtc gcaggctcac gctctccgaa ctccggccgt tcgatctctc ccagcccatc
     1981 tgctatgcag acctcgcgat gcagtgtggt tcgaggacct tcgactccgt gggctgccgg
     2041 ccgcactggt cttgacccgg cgaccaagag gcgattcgag cccg
//
LOCUS       DBNGZC010001054         3371 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1462614, whole genome shotgun sequence.
ACCESSION   DBNGZC010001054 DBNGZC010000000
VERSION     DBNGZC010001054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1462614"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="AC16G0_18155"
     CDS             complement(<1..201)
                     /locus_tag="AC16G0_18155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HIR5101583.1"
                     /translation="MRGLGAMVLVGMLMLQGCVAVAVGLVAGAAAGVTYTVLGNAEKT
                     FDEDFDSVTVALQKALTSLDIKT"
     gene            complement(225..908)
                     /locus_tag="AC16G0_18160"
     CDS             complement(225..908)
                     /locus_tag="AC16G0_18160"
                     /inference="COORDINATES: protein motif:HMM:NF024938.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HIR5101584.1"
                     /translation="MTTPPPASRNRLLPEPEALDFLQSAGLSVIPHRLARTPEEAAAA
                     ARDLGCPVAAKIAAHALAHQSDIGGVILNLSTPEEAAAAFTTLRNRLAARLASIQFHG
                     TMIAKMAPPGGLEVILGMARDPQFGPALMFGLGGVFVEVYNDVVFRLPPLTPAEARSM
                     LTEVKAARLLIGYRGLPARDLESLAAGVCTLARLAADHPEIQEIDLNPVIAYERGCVI
                     VDAKILVRP"
     gene            complement(905..2230)
                     /locus_tag="AC16G0_18165"
     CDS             complement(905..2230)
                     /locus_tag="AC16G0_18165"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012965984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043758 - acetate-CoA ligase (ADP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HIR5101585.1"
                     /translation="MTHPLHSLFCPASVAVVGASGAPEKYGYIILKNILDAGFHGPIY
                     PINPKAAEILGRPCRPRIADLPRGVDLAVIIIPARTVPQAVAECGERGIKSAVVISGG
                     FAESGDEGGLLQREVVETARRSGVRLLGPNCQGVNNPHHNLCASWPLLTQRGEIALIS
                     QSGTVGAALMDWASEEQLGSSAFVSLGNRADVDEADLIEYLAQDPHTRVIALYLEGIK
                     DLERFRRTARSCPKPIVILKSGRTSEGRQAVASHTGSLAGRDELYSALFRQLRIHRAD
                     TLEELYDAAKVLAYLPRPAGRRLLVITSSGGSGILAADWAEWSALEIPPLPPQVAETV
                     RALVPPHCIVSNPLDLTGDATAARYRQTLDATASHFDAQVIIFGDPIQEAAEVVTPGA
                     SQLVIFLGGAEVERRERLRMHQKKVPVFPTPERGLRAYARLLAFEEVPR"
     gene            2455..2688
                     /locus_tag="AC16G0_18170"
     CDS             2455..2688
                     /locus_tag="AC16G0_18170"
                     /inference="COORDINATES: protein motif:HMM:NF013224.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HIR5101586.1"
                     /translation="MNKAHVLVRLTPGLETSAVGQIRTIPGVVDIIMVFGRWDAIVQA
                     EAKTLPALSRLIVSQIRGIQGVQATETLVTAEL"
     gene            complement(2725..3039)
                     /locus_tag="AC16G0_18175"
     CDS             complement(2725..3039)
                     /locus_tag="AC16G0_18175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5101587.1"
                     /translation="MTASRIEALEQMLLRNPEDPTLHYMLGNEYFKAQIYAQAVTALR
                     RYLSLADDEGAAYRLLAQSLDRLGKLDEARRAYQDGLTVATRHGHQPMIEEYTQALKD
                     LE"
BASE COUNT          598 a         1060 c         1156 g          557 t
ORIGIN      
        1 ggtcttgatg tccagactcg tgagggcctt ctggagcgcg accgtcacgg agtcaaagtc
       61 ctcgtcgaag gttttctctg cgttccccag gaccgtgtag gtcaccccgg ccgcggcgcc
      121 ggccacgagc ccgaccgcca ccgccacgca gccctgcagc atcagcatcc ccacgaggac
      181 catcgctccc aatccgcgca tgctcgtctc ccctgtcctc aacttcacgg ccgcaccagg
      241 atcttggcat ccacgatcac gcatccccgc tcgtaggcga tgacggggtt caaatcgatt
      301 tcttggatct cggggtgatc cgcggcgagg cgggccaacg tgcagacgcc ggcggcgagg
      361 ctctccaggt cgcgcgctgg aaggccacgg tatccgatca agaggcgggc cgccttcacc
      421 tccgtcagca tgctccgagc ctccgccgga gtcaagggcg ggaggcgaaa gaccacgtcg
      481 ttatacacct caacgaacac gccacccagg ccgaacatca gcgcgggacc gaactggggg
      541 tcgcgcgcca tgcccaggat aacctcgagg ccaccgggag gggccatctt ggcgatcatc
      601 gtgccgtgaa actgaatgct tgccagacgg gcggccagac ggttgcggag cgtggtgaat
      661 gccgccgcgg cttcctcggg tgtggagaga ttcaggatca ccccaccgat gtcgctctgg
      721 tgcgcgaggg cgtgcgccgc gatcttggcg gcgacagggc agcccagatc gcgggcggct
      781 gcggcggcct cctcgggtgt ccgggccagt cggtggggaa tcacggacag accggcgctt
      841 tgcaggaaat ccagcgcctc gggctccggc agcaggcggt tcctgctcgc gggcggtggc
      901 gtggtcaccg ggggacctcc tcgaaggcga gcaacctggc gtaggcgcga agcccgcgct
      961 ccggcgtggg gaagaccggg accttcttct ggtgcattcg gagccgctcc ctccgctcca
     1021 cctcggcccc gcccagaaag atgacgagct gggaggcgcc gggggtgacg acctcggctg
     1081 cctcctgaat gggatcgccg aagatgatga cctgagcgtc gaagtgggac gccgtggcgt
     1141 ccagcgtttg gcggtaccgg gcggcggtgg catcccccgt cagatccagc ggattggaga
     1201 cgatgcagtg gggagggacg agcgcgcgca cggtctctgc cacctggggg ggcagtggcg
     1261 ggatctccaa tgccgaccat tcggcccaat cggcggccaa gatcccagac ccgccggaac
     1321 tggtgatcac cagcaggcgg cgccccgcgg gccgtgggag ataggccagg accttggcgg
     1381 cgtcgtagag ctcctccagg gtgtccgccc gatgaatcct gagttgccgg aacagagcgc
     1441 tgtagagttc atcccgaccg gcaagagacc ccgtgtggga ggccacggcc tgccgcccct
     1501 cgctggtgcg gcccgatttc aggatcacga tgggcttcgg gcaggaacga gccgtcctgc
     1561 ggaatcgctc cagatccttg atcccttcga gatagagggc gatgactcgc gtgtggggat
     1621 cctgggcgag gtactcgatc aagtcggcct catccacgtc tgcgcgattc ccgaggctga
     1681 cgaaggcgga ggagcccaac tgctcctcgg aggcccagtc catcagcgcc gcacccaccg
     1741 tcccgctctg ggagatgagg gcgatctccc ctctctgggt gagcaggggc caagaggcgc
     1801 acaggttgtg gtgggggttg ttcaccccct ggcagttcgg tccaaggagc cggacgccgg
     1861 agcggcgtgc cgtctccacg acctcgcgct ggagcaggcc gccctcgtct ccggactcgg
     1921 caaatccccc gctgatcacg acggcagact ttattccgcg ctcgccgcac tcggccaccg
     1981 cctgcggcac cgtccgggcc ggaatgatga tgaccgccag gtccaccccg cgggggagat
     2041 cggcgatgcg cgggcgacag ggacggccca ggatctcggc ggccttgggg ttgatgggat
     2101 agatcggccc atggaagccg gcatccagaa tgttcttgag aatgatgtag ccatacttct
     2161 ctggcgcgcc ggacgcgccg acgacagcca cgctggcagg gcagaacagg gagtgcaggg
     2221 ggtgagtcat gatattccac cagggataga catatgcggg acagcacggg cgctgtgtag
     2281 catgcgcgcg cgaggattgt caaggcggcg gtaggcggac cggaacagag cctcgggctt
     2341 ttcacttgac aatttcggcg ctgccttgcg ataagaacaa acagcctgta cctgtgcgaa
     2401 gaaactgcac cgcaggacaa gccggcagga caacggtgga ccagggaggc gaccatgaac
     2461 aaggcgcacg tgctggttcg gctcacgccg gggctggaaa caagtgcggt ggggcagatc
     2521 cggaccattc cgggtgtggt ggatatcatc atggtctttg ggcggtggga tgcgatcgtc
     2581 caggcggagg ccaagaccct gccggctctg tccagattga ttgtcagtca gatccggggg
     2641 atccagggcg tccaggcgac ggagaccttg gtcacggcgg agctatagcg gagcggcgga
     2701 gtctggcgtc ggcaaccggc ggcgctactc cagatccttc agcgcctgcg tgtactcctc
     2761 gatcatgggc tggtggccgt ggcgcgtggc caccgttagc ccgtcctgat aggcgcggcg
     2821 cgcttcgtcg agcttcccca gcctgtccag ggactgggcg agcaagcggt aggcggcccc
     2881 ctcgtcgtcc gcgagcgaaa gataccggcg aagcgccgtg acggcttggg catagatttg
     2941 cgccttgaag tattcattgc ccagcatata atgaagcgtc ggatcctccg ggttccggag
     3001 cagcatctgc tccagggcct cgattcgtga agccgtcatt ccggtgcctc ctctgaatct
     3061 aggagcaacc ttcattcctg gttgcatcat agtcaagatt gtcccgaaca tcaagacgct
     3121 ccggatgtgc ctgccgtcct ccctttgggc tggccggcag caaagggctt gcgccagggt
     3181 ccccccgacg ggcttcgcgg aaatctgatg ggcggcaaag ggcaacgaag accaatgggc
     3241 ggcacggacg ccatgggcac gctgaaacag cgcgtggccg cctttgaaca gcagaatgag
     3301 gagttgtcca actccctgaa ggagttgcgg gcctactacg aggacctgct ctcgagcctg
     3361 caggacggcg t
//
LOCUS       DBNGZC010001055         1457 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2733904, whole genome shotgun sequence.
ACCESSION   DBNGZC010001055 DBNGZC010000000
VERSION     DBNGZC010001055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1457)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2733904"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..505
                     /locus_tag="AC16G0_18180"
     CDS             284..505
                     /locus_tag="AC16G0_18180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101588.1"
                     /translation="MGTRLKRIVKVLLCLCAIELFLPGGTLIVLGYLLTGGRHIHTSA
                     KSEGGLQTWRHIRGLVFANTAGGQPACRV"
     gene            668..910
                     /locus_tag="AC16G0_18185"
     CDS             668..910
                     /locus_tag="AC16G0_18185"
                     /inference="COORDINATES: protein motif:HMM:NF038399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP-related RiPP peptide"
                     /protein_id="HIR5101589.1"
                     /translation="MSQRDVERTLGRLLTDQGFRDDFFSDPAHACLQVGIQLTPQEVE
                     ALLQAPRPALVSLGARLDDRICRLHIQGGEIASQGA"
     gene            922..1308
                     /locus_tag="AC16G0_18190"
     CDS             922..1308
                     /locus_tag="AC16G0_18190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101590.1"
                     /translation="MLYKHSAAMAKAEPAGMALRGNARRSGPWHGGPRGLSRIIQYTD
                     EAVPSNEYPERIVSPPRAAACCALSMVNVGDPEEETGWLFQYRRCQRCGFTVRRILRE
                     VPDEELLAELRRSLAHAFVRNAREAA"
BASE COUNT          259 a          440 c          479 g          279 t
ORIGIN      
        1 cgccgcttac tcgcttccaa ctctacaccc gcctgaagcg ctaccacatc gacgttccct
       61 ccgactagtc tgcgggactg ctttcgagcg gtgtctccag caacgtgcaa taccgcacgg
      121 gcgcgtgcag agtcgcactc ctgggtgtgt aagtcttcct cccccagtca tggtcgcgat
      181 ttttcggcat gttacgcact cggattattg gcatgcgctg tgcttttcca aaggcgtgaa
      241 acaccccacg acgataacta cgagatagga gatgatcaca ggaatgggta cacggctcaa
      301 gcggattgtc aaggtgctgc tctgcctgtg tgccatcgag ctgtttctgc ccggcggcac
      361 tctgatcgta ctgggctact tgctgacggg tggacgccac atccacactt cggccaagag
      421 cgagggaggt cttcagacct ggcgccatat tcgggggctc gttttcgcga acacggctgg
      481 cggccaaccg gcgtgcaggg tgtgaagtgc cgccggttgc cggggagagc tgagatgtct
      541 tggacatgga tgtggattgg aggatcgctg gtgctgggat ttcacctcgg cgtgacgctc
      601 atgagcctgt tggtcatttc acggacccgc gggtgagtga ggaagtcagg ataacaagga
      661 ggacatgatg agtcagcggg atgtggagcg gaccctgggc cgattgctca cggatcaggg
      721 cttccgggac gatttcttct cggatccggc ccacgcctgt ctacaggtcg ggatccagtt
      781 gacgccgcag gaggtcgagg cgctcctgca ggccccccgc ccggcgctcg tgagcctcgg
      841 cgcccgcctc gatgaccgga tctgtcggct ccacatccag gggggcgaga tcgcgagcca
      901 gggggcgtga cgggggatgc catgctgtac aaacacagcg ccgccatggc gaaggcagag
      961 cctgcgggca tggccttgcg tgggaatgcg agaaggtccg gcccatggca cggggggccg
     1021 cgcggtctat caaggatcat tcagtacacg gatgaagcgg tgccgtccaa cgaataccct
     1081 gagcggatcg tctccccgcc tcgggccgcc gcctgctgcg ccctcagtat ggtgaacgtc
     1141 ggggacccgg aggaggagac tggctggctc tttcagtacc gccggtgcca gcggtgcggc
     1201 ttcacggtcc gccggatcct ccgcgaggtc cctgacgaag aactgctggc ggaactccgg
     1261 cgctccctcg cccatgcctt tgtccgcaac gcgagggagg cggcgtagcg tgtcggggat
     1321 cggtgtcgtc agtccgaacc ggagcaaggc ttacggagat aacaccagtg aaccctgaaa
     1381 agaagaccga taggttcatc acactcggcg ggcccccgcc cgtcccgtac tgcccccagt
     1441 tccgatggtt cgtgctc
//
LOCUS       DBNGZC010001056         5052 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_190218, whole genome shotgun sequence.
ACCESSION   DBNGZC010001056 DBNGZC010000000
VERSION     DBNGZC010001056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_190218"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="AC16G0_18195"
     CDS             complement(<1..1102)
                     /locus_tag="AC16G0_18195"
                     /inference="COORDINATES: protein motif:HMM:NF018513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR5101591.1"
                     /translation="MVIAVIFACFFLLLALRVPVGFSLGIVSMGFLIARGEVLLAVSQ
                     RMSAAADSFPLLAIPFYIIMGKLVNEAGLTDRIFGFARSLVGHIRGGLAHVVILNSMV
                     FAGMSGAAVADVAGMGAMQIKAMTDNGYDKDFSAAVTAAGSTIAPIIPPSISMVVYGV
                     LASTSVGALFMGGFIPGFLMGGFMMVYSYILAIRRNYPLSPRATLRELLSSFVYSIPG
                     LMTPVILLSGIAFGIVTPTEAAVVAVFYTGIVGIVVYRRLGIGAIIRILRETIQTTGV
                     ICFVMTTASLFAWIMAREQVPQRFAEFILSITDSPAVFLLFINILGLIMGCFFDGISI
                     LVIVVPIVLPILDKMGIDRIHFGVMFTLNTVIG"
     gene            complement(1096..1590)
                     /locus_tag="AC16G0_18200"
     CDS             complement(1096..1590)
                     /locus_tag="AC16G0_18200"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR5101592.1"
                     /translation="MDLVERLLAGIDWITDKVTLLILILLNLIVGLQVFSRYVLNHSL
                     FWSEELARYLFIYLVFLSGAMVLRQDRHIQVTALVDILPAPLKRAIIVLGELLMLSFA
                     GVVLVESIRLAAMVGTVLTAAMEIPWSLVYLGIILGMAIMVLALVGSLWARLTGRREE
                     RGAW"
     gene            complement(1595..2569)
                     /locus_tag="AC16G0_18205"
     CDS             complement(1595..2569)
                     /locus_tag="AC16G0_18205"
                     /inference="COORDINATES: protein motif:HMM:NF015445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid TRAP transporter substrate-binding
                     protein SiaP"
                     /protein_id="HIR5101593.1"
                     /translation="MNSRWIVALLVAVAFLVVVPQASAQVVIKWGVVLAPEHPFTLGM
                     KKTAEIVAQKTNNRIQIQIFPGAQLGTGKDMIEATVFGSQSMVTEGAAMFSQWAPRLS
                     IMEAPYLFRDVNHMIKAIKSPVGQEMVDELTAKRGLRSLGVVYYGVRHLTANKPVHAP
                     QDVKGLKLRVPEVPLYLEMAKSWGANPTPMAFAELYLALKQGTVDAEENPIPTINAGK
                     FYEVQKYLILTGHIMVPQLHVISDKVWKSLAAADQKILQEAVDEGIAYSTDMLIKQEQ
                     TQAEEFKKKGMQVITPDVAAFKKASMAVIPKMEGVWGKGLYEKLAEIR"
     gene            complement(2651..3565)
                     /locus_tag="AC16G0_18210"
     CDS             complement(2651..3565)
                     /locus_tag="AC16G0_18210"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012904.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR5101594.1"
                     /translation="MSSITHPSSKSRLSGIVAALLTPMRDGGTRLDPEAASRLGSWLV
                     ERGSHGLYVAGTTGEGLYLSSDEHRELTRAVVKAARGIPVVVHVGALTTATAVTLARQ
                     AVQAEASAVAAIPPSYYSLTRAELVAYFTAIGKAAEPLPVYLYNIPSHARNDLRPELV
                     KDIRQAVPNVTGIKDSSGFPGRIVDLVRTMGRGFDVVCGNDEKDLSAFQDGAMGIVSS
                     GAGVFPELYLELYAAWKAGRLAEAQAAQGRIVAMQEALGNGARLGWYKHVLAQRGVPI
                     GGVRAPLLDPTPAEQEAMRVRLTELKLL"
     gene            complement(3558..4973)
                     /locus_tag="AC16G0_18215"
     CDS             complement(3558..4973)
                     /locus_tag="AC16G0_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HIR5101595.1"
                     /translation="MQKRKIGILTFSDGRKFAHDLQYGMNRGFQERLVKTLEATGEVE
                     CVAGELIWTPELARSEAKKLAAAGVEATILNFAIWAFPHFPAIATEFAPGPFLCLSNV
                     NPQAPGLVAMLAAAGTLNQIGVFHARVSGDVEEPAVLRKVLAFLRAASAVNRLKGKTY
                     GLFGGRSMGMYTAVPALDQWRREFGVDIEHLDQWEIVRRSEGIDQDRVTRARQWLEKH
                     CGQVAYDGKALTPESLERQIRSYHAVRELIREWDLDFVGIKGQPELTNSFCTMDLTEA
                     FLNDPYDWEGPHDPIVCATEADSDGALSMQLFKELAGTPVLFADVRHYDADGDFWDMV
                     NSGQHATYFAGRSFDPAVNLPKVKLLPENFYFPAGGAAVHHVAAPGEVTLARLTRKRG
                     KYWMAVAPAEFLALPPGQLEAKVRATTGEWPHAFTRLRVPPDRFIATYSSNHIHGVYG
                     DYVEELKWVCKILGMEAEIYV"
BASE COUNT          940 a         1685 c         1561 g          866 t
ORIGIN      
        1 gcccgatcac cgtattgagg gtaaacatca cgccgaaatg aatccggtcg atgcccatct
       61 tgtccaggat gggcaggacg ataggcacca cgatgaccag gatggagatc ccgtcgaaga
      121 agcaccccat gatcaggccc aggatgttga tgaacaggag gaagactgcc gggctgtccg
      181 tgatcgacag gatgaactcc gcgaagcgct gggggacctg ctcgcgagcc atgatccagg
      241 cgaacaggga ggccgtggtc atcacaaagc agatcacgcc cgtggtctgg atcgtctcgc
      301 gcaggatgcg gatgatcgcg ccgattccca gcctgcgata gaccacgatg cccacgattc
      361 cggtatagaa gacggcgacg accgcggctt cggtgggggt gacgatgccg aaggcgatcc
      421 cgctcaggag gatcaccggg gtcatcaggc ccgggatcga gtacacgaag ctcgagagca
      481 gttctcgcag ggtcgcccgg ggcgacagag gatagttgcg acggatggcc aggatgtacg
      541 aatacaccat catgaaaccg cccatcagaa agccggggat gaatccgccc atgaacaggg
      601 ccccgaccga ggtgctggcc aggaccccgt acaccaccat ggagatgctg ggcgggatga
      661 tcggggcgat ggtggagcct gccgccgtga cggcggcgga gaaatccttg tcgtacccgt
      721 tgtcggtcat cgccttgatc tgcatcgccc ccatgccggc cacatccgcc accgctgcgc
      781 ccgacatgcc ggcaaagacc atcgagttca ggatgacgac gtgggccaga ccgccgcgga
      841 tatgccccac cagggatcgg gcaaagccga agatcctgtc cgtgagcccc gcctcgttca
      901 ccagcttccc catgatgatg taaaacggga tggccagaag cgggaaggaa tccgcggcgg
      961 ccgacatccg ctgggagacc gccagcaaga cctcccctcg cgcgatcagg aatcccatcg
     1021 agacgatccc cagggagaaa ccgacgggaa cgcggagcgc caggagcaag aagaagcagg
     1081 caaagatgac cgcgatcacc atgcgccccg ctcctctctc cgacccgtga ggcgcgccca
     1141 aagcgatccc accaacgcca gcaccatgat cgccattccg agaatgatgc cgagatagac
     1201 aagggaccat gggatttcca tggctgccgt caggacggtc cccaccatgg cggccagccg
     1261 gatgctttcc accagaacaa cccctgcgaa gctgagcata agcagctccc ccagcacgat
     1321 gatcgcgcgc ttgagggggg cgggcaggat gtccacaagg gcggtcacct ggatgtgccg
     1381 gtcctgccga aggaccatcg cccccgacag gaagaccagg tagatgaaca agtagcgcgc
     1441 cagctcctcc gaccagaaga gggagtggtt cagcacatac cgggagaaca cctgcaaccc
     1501 gacgatgagg ttcaggagga tcaggataag cagcgtgacc ttatccgtga tccagtcgat
     1561 gcccgccagg agccgctcca cgaggtccac ggcctcaccg gatctccgca agcttttcgt
     1621 acagcccttt gccccacacc ccttccatct tggggatgac cgccatggac gccttcttga
     1681 aggcagccac atccggggtg atcacctgca tccccttctt cttgaactct tccgcctgcg
     1741 tctgctcctg cttgatcagc atgtcggtgc tgtatgcgat cccctcgtcc acggcctcct
     1801 gcaggatctt ctggtcggcc gccgcgaggc tcttccagac cttgtcgctg atcacatgga
     1861 gctggggtac catgatgtgc ccggtgagga tcaggtactt ttgcacctcg tagaacttcc
     1921 ctgcgttgat ggtggggatg gggttctcct cggcgtccac cgtgccctgt ttcagggcca
     1981 gatacagctc ggcgaaggcc atcggcgtcg gattggcgcc ccaggatttg gccatttcca
     2041 ggtagagcgg cacctcgggg acgcggagct tgaggccctt cacgtcttga ggcgcatgga
     2101 caggcttgtt ggccgtcaga tgccggaccc cgtagtacac gacccccaat gagcgcaggc
     2161 cgcgcttcgc cgtcaattcg tccaccatct cctggccaac ggggctcttg atcgccttga
     2221 tcatgtggtt cacatcacgg aagagatagg gcgcctccat gatggagagc cggggcgccc
     2281 actggctgaa catggccgcg ccttcggtga ccatggattg gctgccgaag accgtcgcct
     2341 cgatcatgtc tttgccggtt ccaagctggg cgccggggaa gatctgaatt tgaatccgat
     2401 tgttggtttt ctgcgcaacg atctccgccg tcttcttcat gcccagggtg aaaggatgct
     2461 ccggcgccag cacgaccccc cactttatga ccacctgtgc cgaggcctgc ggcacgacga
     2521 caaggaacgc cacggcgacc aagagcgcca cgatccatcg agagttcatt gcgtgttcct
     2581 ccttctgaga cctccgttca gccatgcgat ccccttgccc accgggtgtt cgggtctcaa
     2641 ccccccaccc ctagagcagc ttcagctctg tcaatcgcac tcgcatcgcc tcttgctcgg
     2701 ccggggtcgg gtcaagaagc ggcgcgcgaa ccccgcctat cgggacaccg cgctgcgcca
     2761 ggacgtgctt gtaccagccc aggcgagcac cgtttccgag ggcttcctgc atcgccacga
     2821 tacggccttg cgcagcctgc gcctccgcca ggcgaccggc cttccaggcc gcatacagct
     2881 ccaggtacag ttcggggaat acccccgcgc ctgacgagac gatgcccatc gcaccgtcct
     2941 ggaaggcgct aagatccttc tcgtcgttcc cgcagaccac gtcgaagccc cggcccatcg
     3001 tcctgaccag atcgacgatc cgccccggaa agccgctgct gtccttgatt cccgtcacgt
     3061 tcggaacggc ctggcggatg tccttgacca gctcaggacg caaatcattc ctggcgtggc
     3121 tcggaatgtt gtagagatac acgggaagcg gctccgcggc cttgccgata gctgtgaagt
     3181 aggcgaccaa ttccgcccgg gtcaggctgt agtagcttgg ggggatggcc gccacggccg
     3241 acgcctcagc ctggacggcc tggcgagcta gcgtcaccgc cgtggccgtg gtgagcgccc
     3301 ccacgtggac caccaccggg atcccgcgcg ccgccttcac gaccgcccgc gtcaactccc
     3361 gatgctcgtc cgagctgagg taaagcccct ctcccgtggt ccccgcgacg tacagtccgt
     3421 gactgccccg ctcgaccagc caggatccca ggcgggaggc ggcctccggg tccaggcggg
     3481 tgcctccgtc ccgcatgggc gtcaagaggg cggcgacgat gccggacaga cgtgatttcg
     3541 atgacggatg cgtgatgcta gacatagatc tcggcctcca tccccagtat cttgcacacc
     3601 cacttgagtt cttccacgta gtcgccgtag accccgtgga tgtggttgga gctatacgtg
     3661 gcgatgaacc ggtctggcgg gacgcggagc cgggtgaagg cgtgcggcca ctccccggtg
     3721 gtggcccgca ccttcgcctc cagctgtccc ggcggcaggg ccaggaactc ggcgggggcg
     3781 acggccatcc agtacttgcc acgcttgcgc gtcaggcgag ccagagtgac ctcgcccggc
     3841 gcggccacgt ggtgcaccgc cgctccaccc gccggaaagt agaaattctc cggtagcagc
     3901 ttcaccttgg ggaggttcac cgcggggtcg aaggaccgac cggcgaaata ggtggcgtgc
     3961 tggcccgagt tcaccatgtc ccagaaatct ccgtcggcgt cgtagtgccg gacatccgcg
     4021 aagaggacgg gcgtccccgc cagctccttg aagagctgca tgctgagggc gccgtccgaa
     4081 tccgcctcgg tggcgcagac gatcgggtca tgcggccctt cccaatcgta aggatcgttc
     4141 aggaacgcct cggtgaggtc catggtgcag aacgagttgg tcagctccgg ctgtcccttg
     4201 atcccgacga agtccaggtc ccactcccgg atcagctcgc ggacggcatg gtaactccgg
     4261 atctgccgct ccaggctctc gggtgtgagg gccttcccat cgtaggccac ctggccgcaa
     4321 tgcttttcca accactgccg agcccgggta acccggtcct ggtcaatccc ctcggagcga
     4381 cgcacgatct cccactggtc caggtgctcg atgtccaccc cgaactcgcg ccgccactgg
     4441 tccagggcag gaacggcggt gtacatcccc atggaccggc cgccgaagag gccgtaggtc
     4501 ttgcccttca gccggttcac ggccgaggcc gcccgcagga aggccaggac cttccgcaac
     4561 acggcgggct cctcgacgtc gcccgagacc cgcgcgtgga acaccccgat ctggttcaac
     4621 gtgccggccg cggccagcat ggcgaccagg cccggcgcct gggggttcac gttggaaagg
     4681 cagaggaagg ggccgggggc aaactctgtg gcgatggccg ggaagtgggg gaaggcccag
     4741 atggcgaagt tcaggatggt ggcttcgacc cctgccgcag ccagcttctt cgcctcggaa
     4801 cgcgccagct ccggcgtcca gattagttcg ccggccacgc attccacctc gcccgtggcc
     4861 tccagggtct ttaccaggcg ctcctggaag ccccggttca tgccgtactg caagtcgtga
     4921 gcaaacttcc gcccgtctga gaacgtcagg attccaatct tgcgtttttg cattgcagcc
     4981 tcctctcacg cgttcggccg gggcctcctc aggatgcggc ccccagctcg gccagggcgt
     5041 gcatcgtggc cc
//
LOCUS       DBNGZC010001057         2097 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1655677, whole genome shotgun sequence.
ACCESSION   DBNGZC010001057 DBNGZC010000000
VERSION     DBNGZC010001057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1655677"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1307)
                     /locus_tag="AC16G0_18220"
     CDS             complement(<1..1307)
                     /locus_tag="AC16G0_18220"
                     /inference="COORDINATES: protein motif:HMM:NF015089.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR5101596.1"
                     /translation="MSHQPPAFHKNAVLFGHDPTPRLLAFELEGDDRIRVFTRTPDGR
                     LESQTHPFRPFVLLSSTELLSGWTGGFDVEALTGDGLYRFLALFHGWDDALKARAHLT
                     KITGKAQTAPDAPFLFFSDPIQQHLMLSGQTHFLELPFRDLRRMQLDIETYCATGYEF
                     PNAARAEDRITAIALSDNTGWEHVISGTQLAEPDMLRELVRLVTERDPDVIEGHNFFR
                     FDLEYITARAARHKVKLHLGRGGAVLRGHPSRMQIAERTITYKKFEVFGRHIIDTWIL
                     AQHYDVTARELEGYGLKEIARHFGLATDERTYILPEKVSWYFDHEPDTLFRYALDDVR
                     ETRGVSEILSQSYFIQAQIFPFSYQNVALRGNATKIDALLIREYLRQRRAIPAPEPPQ
                     EVLGGHTEIAHQGVARRILHCDVTSLYPSVMLTFGAFPRRDSLG"
     gene            complement(1307..1738)
                     /locus_tag="AC16G0_18225"
     CDS             complement(1307..1738)
                     /locus_tag="AC16G0_18225"
                     /inference="COORDINATES: protein motif:HMM:NF020967.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative signal transducing protein"
                     /protein_id="HIR5101597.1"
                     /translation="MFCPVCGSEYIAGVATCSDCDVPLIPGPPPGDDLADEPIFDDPV
                     VMAIEVFSPAEAEIVCALLQAHGIPCILKAENQYAVDATYTVGPLARRRILVRESDLE
                     RARELLRAAPQPDPCMLSEGESPQAPQTPEEEPSVPSTSDR"
     gene            complement(1827..>2097)
                     /locus_tag="AC16G0_18230"
     CDS             complement(1827..>2097)
                     /locus_tag="AC16G0_18230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101598.1"
                     /translation="VPRLAQPRTPRKGPADTQATTIAGRSGFGSCIGLVGVAAATYVT
                     WPANTLGDYLHHRRTQHEIIRSLERRIGAEPDVLMRGFYQAWLAE"
BASE COUNT          349 a          654 c          738 g          356 t
ORIGIN      
        1 cccaggctgt cacgccgcgg gaaggcgccg aaggtcagca tcaccgacgg atagagggat
       61 gtcacgtcgc agtgcaggat gcggcgggcc accccctggt gggcgatctc ggtgtgcccg
      121 cccaggactt cctgcggggg ctccggcgcg gggatggccc gacgctggcg caggtattcc
      181 cggatcagca gggcgtcgat cttggtggcg ttcccccgca gggccacgtt ctggtaggag
      241 aacgggaaaa tctgcgcctg gatgaagtag ctctggctca ggatctcgct caccccgcgg
      301 gtctcccgga cgtcgtccag ggcgtagcgg aacagggtgt ccggttcgtg gtcgaaatac
      361 cacgagacct tctcgggcag gatataggtc cgttcatccg tggccaaacc gaagtgcctg
      421 gcgatctcct ttaggccgta cccttccaac tcgcgcgcgg tcacgtcgta gtgctgggcc
      481 aggatccagg tgtcgatgat gtggcggccg aagacctcga acttcttgta ggtgatcgtc
      541 cgctcggcga tctgcatgcg ggagggatgc ccccgcagga ccgccccacc ccgccccagg
      601 tgcagtttca ccttgtgacg cgccgcccgg gctgtgatgt actccagatc gaagcggaag
      661 aagttgtgcc cctcgatgac atccgggtct cgctccgtga cgaggcggac cagttcccgg
      721 agcatgtcgg gctcggccaa ctgcgtgccc gagattacgt gctcccagcc ggtgttgtcc
      781 gagagggcga tggcggtgat gcgatcctcc gcccgcgccg cgttcgggaa ctcgtagcct
      841 gtagcgcagt aggtctcgat gtccagctgc atccggcgca ggtcccggaa cggaagctcc
      901 agaaagtggg tctgcccgga gagcatgagg tgctgctgga tgggatcgct gaagaacagg
      961 aaaggcgcgt cgggggcggt ctgcgccttg cccgtgatct tggtgaggtg cgcgcgggcc
     1021 ttcagggcgt cgtcccagcc gtggaacagg gccaggaacc ggtacagacc atcgcccgtc
     1081 agcgcctcca cgtcgaagcc gccggtccag cccgaaagca gctccgtgct ggaaagcagg
     1141 acgaagggcc ggaaggggtg ggtctgggac tcgaggcgcc cgtccggtgt gcgtgtgaag
     1201 acccggatac ggtcgtcccc ctccagctcg aaggccagca ggcgaggcgt cggatcgtgg
     1261 ccgaacagga ccgcgttctt gtggaaggcc gggggctgat gactcattac cggtcagacg
     1321 tggaaggcac gctgggttcc tcctcgggcg tctgcggcgc ttgcgggctc tccccttcgg
     1381 acagcatgca ggggtcgggc tgaggggcgg cccgaaggag ttcccgggca cgctcgagat
     1441 cactttcgcg gaccaggatg cgtcggcgcg ccagcgggcc cacggtgtac gtggcatcca
     1501 cggcatactg gttctccgct ttgaggatgc agggaatgcc gtgggcttgg agcagggcgc
     1561 agacgatctc ggcctcggcg gggctgaaga cctcgatggc catgacgacc ggatcgtcga
     1621 agatcggctc gtcggccagg tcgtcgcctg gcggcggacc gggaatcagc gggacatcgc
     1681 agtccgaaca ggttgcaacg cccgcgatat actcactccc gcagaccgga cagaacatgt
     1741 gcggctcgtg cctcggggtg cagaaggggc cctcgccggg tccagaagga ctcaagactc
     1801 tggatggcct gggccaagtc ccccttttac tcggccagcc aggcctggta aaatccgcgc
     1861 atcaagacat cgggctccgc cccgatccgc cgctccaggc tgcggatgat ctcgtgctgg
     1921 gtgcggcggt ggtgaagata atcccccagg gtgtttgccg gccatgtcac gtacgtcgcg
     1981 gccgcgaccc cgaccaagcc gatgcaggac ccgaaaccgg aacgcccagc aatcgtcgta
     2041 gcttgggtgt cagcgggccc cttccgcggt gtccgaggct gagccagcct gggtact
//
LOCUS       DBNGZC010001058         7586 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1655691, whole genome shotgun sequence.
ACCESSION   DBNGZC010001058 DBNGZC010000000
VERSION     DBNGZC010001058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7586)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7586)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7586
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1655691"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="AC16G0_18235"
     CDS             complement(<1..237)
                     /locus_tag="AC16G0_18235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5101599.1"
                     /translation="MSAPGVRILTARWVFPVTSPPIADGAVAIQGGEILAVGPRDEVA
                     RAFSGTPRWDLGDAAILPGLVNCHTHLELGGLMRP"
     gene            complement(270..917)
                     /locus_tag="AC16G0_18240"
     CDS             complement(270..917)
                     /locus_tag="AC16G0_18240"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HIR5101600.1"
                     /translation="MTAPMQGSPNRAMTGAILAGGRSTRMGTNKAVLDFGGQRLIVGL
                     LQKIRPLFPEVVIIANEFAPYADLEVSMFSDCIPDKGSLGGIYTAVLHSTFPQTFCIA
                     CDMPLASPAVIAYLRDQGPGYDVVVPRTEEGYQPLHAVYGKACLPRMEAMIRADNLKI
                     DRLFPAVRVRTVEAEELRRLDPSLRCFVNVNTREELAAAMRLAAEGKDESRGGQP"
     gene            complement(914..1573)
                     /gene="mobB"
                     /locus_tag="AC16G0_18245"
     CDS             complement(914..1573)
                     /gene="mobB"
                     /locus_tag="AC16G0_18245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HIR5101601.1"
                     /translation="MIPIVSVVGRSNSGKTTLLEKLIRTLADRGWRIGTIKHHFRGPV
                     QVDVPGKDSWRHKQAGARAVALASAQTFFVIRDTDGEIPLETMAHLALFGVDCILTEG
                     FKSGSMPKIEVNRDGPGAPLLCGPDDHLVAVVSDRNRGVPVPHFGPEDVSSLADFIER
                     EFLKPSHSSLVDVLLGGRRVPLDNQTQEILARVIRCLVGDSRDPGSGPPIEIRIHEQT
                     P"
     gene            complement(1628..2074)
                     /gene="rpoZ"
                     /locus_tag="AC16G0_18250"
     CDS             complement(1628..2074)
                     /gene="rpoZ"
                     /locus_tag="AC16G0_18250"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR5101602.1"
                     /translation="MSQVPFEDLLEHVDSKYRLVIVASKRAKQLNRGAVSLIQPKSHK
                     PTYQALEEIASGKLGYEIDALAGTAAGELPEATAKPTWFRSLSAEEVVPEEAVTETEE
                     HFEEEEAAAEAPVAGAADDLLMNEFEEESLEDLGEIDALDHEQTEE"
     gene            complement(2115..2699)
                     /gene="gmk"
                     /locus_tag="AC16G0_18255"
     CDS             complement(2115..2699)
                     /gene="gmk"
                     /locus_tag="AC16G0_18255"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR5101603.1"
                     /translation="MLVVVSAPSGAGKTSLCEWVIKAVPGLAHSVSYTTRPPRPDERD
                     GHDYHFADPAVFQAMVDRAEFAEWAVVHGHLYGTSRALLERYFAAGQDVILDIDTRGA
                     AILRKAHPDAVFVFIVPPSWALLEERLRSRRSDAEAEIQRRLQRAREEVRHYAEYQYI
                     IVNDVFARAAEELKAIILAERRRSSRVDLGFLQT"
     gene            complement(2749..3039)
                     /locus_tag="AC16G0_18260"
     CDS             complement(2749..3039)
                     /locus_tag="AC16G0_18260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006425055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix/biofilm biosynthesis
                     regulator RemA family protein"
                     /protein_id="HIR5101604.1"
                     /translation="MATEVLNIGFGNVVLRSRIVAILSARSSKDSKAVFSEPMKRLRD
                     DAREAGRLVDATCGRRTQTLIVTDSGHVVLSAIQSSTLAQRVGGSDEVRERT"
     gene            complement(3144..4010)
                     /locus_tag="AC16G0_18265"
     CDS             complement(3144..4010)
                     /locus_tag="AC16G0_18265"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIR5101605.1"
                     /translation="MTGFGRGEATRRDRVFTVEVHSVNHRFLEVRCRVPKRLTGLEPR
                     IQQTVQQRFARGHFEVSVLDKDLEGRSRTLRVDVPLALQYVDALRTLQRELQLPGEVT
                     LEMLAAQHELIAVEESEDSLDETWGEVVPALAAALDALTEMRGREGEALVRAIAKHLE
                     EVEAILDGIVVRAPDLVRAQRERLRERVVDLLEGHLPDPTRLEQEVAFLAERGDVAEE
                     CDRLRSHLIQFGKALTQAGPQGRRLDFLIQEMHREANTMGSKAGDATLAQDVVGLKLI
                     VERLREQVQNIE"
     gene            4183..4473
                     /locus_tag="AC16G0_18270"
     CDS             4183..4473
                     /locus_tag="AC16G0_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR5101606.1"
                     /translation="MPIYEYECESCDNRFELMQKFSDKPLKKCVKCGGPVHKVLSAPA
                     LLFKGTGWYVTDYANPDRKRAQKADKDNGGNGDKKAEPAASKATATTNKSNN"
     gene            complement(4481..4927)
                     /locus_tag="AC16G0_18275"
     CDS             complement(4481..4927)
                     /locus_tag="AC16G0_18275"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR5101607.1"
                     /translation="MGVASLLSTDGFSSQQTSRYLIPLLHWVIPGASAASLSLLHAGI
                     RKGMHMFEFGVLAWLWYRALKWGEAGWRPQAAVRAFLLAAAFGTADEIHQVFEPHRGA
                     SAVDVGWDSLGALFGLGSCWMLRGDGEPSGKASAPGGVPSGGVRLG"
     gene            complement(4996..6684)
                     /locus_tag="AC16G0_18280"
     CDS             complement(4996..6684)
                     /locus_tag="AC16G0_18280"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR5101608.1"
                     /translation="MRWSRSLIPTRKEDPADAEAVSHKLMIRAGFVRQLAAGIYVYLP
                     LGQRVMDKINAIIREEINAIGGQEITMPVILPAEIWQQSGRWDTVGSEMFRLKDRNQR
                     DMCLGMTHEEVIAWLAAREIHSYRDLPQLWYQIQTKERDEARPKSGVLRTREFVMKDS
                     YSLDRDMAGLEVQYEAHRKAYCRIYDRCGVKYVVVESDPGMMGGAGSDEFMAPSQAGE
                     DRVALCPSCQYAANVELAASRPPLPAFPNWSLEEVSTPNARTIAEVSRLLQIEPALTI
                     KSLVVVGEGGPALALVRGDQQLHERKLQRILGPLRPAHQEEIEQATGGEAGFVGPVGL
                     SLRTIADACLRTGCYVAGANKPDTHLRGVVAGRDFQAEFADLHEAKAGDGCGRCGAPL
                     QVESVIEIGNIFRLGTKYSIPLRAVYLDEQGHEQPIVMGSYGIGPARIAAAAIEQNHD
                     RYGMIWPESIAPFEVHLLVVNTKDQKMGELAELLYRDLTAAGLDVLYDDRDERPGVKF
                     KDADLLGVPLRVTVGSRAIKEGLIEVRRRRTGEESLVAPAEAVARVRSLLGRAT"
     gene            complement(6772..>7586)
                     /gene="ispG"
                     /locus_tag="AC16G0_18285"
                     /gene_synonym="gcpE"
     CDS             complement(6772..>7586)
                     /gene="ispG"
                     /locus_tag="AC16G0_18285"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR5101609.1"
                     /translation="ALQCLEGGVDCLRINPGNIGATWRVREVTKAAAERRVPIRIGVN
                     AGSLEKHLLQRFGEPTPEAMVESALFHVRILEELNYPEMKISLKSSDPKMMIAAYRLL
                     ADKVPYPFHLGVTEAGTAFTGAIKSAVGIGTLLAEGIGDTIRVSLTEDPTEEIRAGFE
                     ILKALGLRKRGLNVIACPSCGRVEIDLVGLTKEVEKRLEVLNIHRPLNVAVMGCAVNG
                     PGEARGADIGIAGGNGVGILIKNGQIVRKVKESELADVLIEEVKALAARTEE"
BASE COUNT         1245 a         2699 c         2344 g         1298 t
ORIGIN      
        1 cggcctcatc agcccgccga gttccaggtg ggtgtgacag ttcacgagtc ccggcaggat
       61 ggccgcgtct cccaggtccc agcggggggt gccggagaac gccctcgcca cttcgtcccg
      121 cggacccacg gcaaggatct ccccgccctg gatcgccacc gcgccgtcgg cgatcggggg
      181 cgaggtcacg gggaacaccc accgcgccgt caggatacgc acgccagggg cgctcatctc
      241 ggcagcgcct cctgtccctg tccctgtggc tatggctgtc ctcctcggct ctcgtccttc
      301 ccctccgccg ctagacgcat ggcggccgcc aattcctccc gggtgttcac gttgacgaag
      361 cagcggagcg atgggtccag gcggcgcagt tcctccgcct ccacggtccg gacccgaacc
      421 gcggggaaca gccggtcgat cttcaggttg tcggcccgga tcatcgcctc catccggggc
      481 aggcaggcct tgccatagac ggcgtgcaac ggctgatagc cctcctccgt ccgcgggacc
      541 accacgtcgt atcccggccc ttgatctcgg aggtaggcga tgaccgccgg gctcgccagg
      601 ggcatgtcgc aggcgatgca gaaagtctgc gggaatgtgc tgtgaaggac cgcggtatag
      661 attccgccca gggatccctt gtccgggata caatcggaaa acatggacac ctcgagatcc
      721 gcatagggag cgaactcgtt ggcgatgatc acgacctcgg ggaagagcgg gcggatcttc
      781 tggaggaggc ccacgatgag gcgctggccg ccgaagtcca gcaccgcctt attggttccc
      841 atgcgggtgg agcggccgcc ggcaaggatg gcacccgtca tggctcgatt gggcgagcct
      901 tgcatgggtg ccgtcatggc gtttgttcgt ggatgcggat ctcgatcggg gggccgctgc
      961 ccgggtcccg gctgtccccc accaggcacc ggatcacgcg ggccaggatc tcctgggtct
     1021 gattgtccag cgggacccgc cgcccgccca gcagcacatc caccagcgaa gagtggctgg
     1081 gcttcaggaa ctcacgctcg atgaagtccg ccagggagga cacgtcctcc gggccgaagt
     1141 ggggaaccgg cacccctctg ttccggtcgg acaccacggc gaccagatga tcgtcaggtc
     1201 cgcagagcag cggcgcgcct ggcccgtctc ggttcacctc gatcttgggc atggaaccgg
     1261 acttgaaccc ttccgtgagg atacaatcca ccccgaagag ggccaggtga gccatcgtct
     1321 ccagggggat ctcgccatcc gtgtctcgga tgacaaagaa cgtctgggcg gaggcgaggg
     1381 cgacggcccg cgctcccgct tgcttgtggc gccaggaatc cttccctggc acgtccacct
     1441 ggaccgggcc gcgaaaatga tgcttgatcg tcccgatccg ccacccgcgg tccgcgagcg
     1501 ttcggatcag tttctccagc aacgtggtct ttccgctgtt ggatcgcccg acgacggaga
     1561 cgatggggat catcggtcac ccgcaggcca aagggtcggc ggtccggagc ggtccgccgc
     1621 tccgaggcta ctcctcggtt tgctcgtggt ccagggcgtc gatttcgccc aggtcctcca
     1681 ggctctcctc ctcgaactcg ttcatcaaga ggtcgtcggc cgcacccgcc accggggcct
     1741 ctgccgctgc ttcctcttcc tcgaagtgct cttcggtctc cgtgaccgcc tcctcgggca
     1801 caacctcttc ggcgctcagg ctccggaacc aggtgggctt cgccgtggct tcgggcaatt
     1861 cgcccgccgc cgtcccggcc agggcatcga tctcatagcc cagcttgccc gaggcgattt
     1921 cctccagcgc ctgataggtt ggcttgtggc ttttcggctg aatcaaggag acggctcccc
     1981 ggttcagctg cttggcccgc ttggacgcca caatgaccag ccggtacttg ctgtccacat
     2041 gctccaggag atcctcgaat ggcacttgcg acatcgcctg ctcctctccg accgtccccg
     2101 ccccgcggcg gtgatcaggt ctgcaagaat ccaagatcca cccggctgct ccgccgccgt
     2161 tcggccagga tgatggcctt cagttcctcc gcggcccggg cgaacacgtc gttcacaatg
     2221 atgtactggt actcggcgta gtgcctgacc tcctctcggg cccgctgcag gcggcgctgg
     2281 atctcggcct cggcgtcgct tcgccgcgac cgcagccgct cttccaggag ggcccaggac
     2341 ggcggcacga tgaacacgaa gaccgcgtcc ggatgggcct tccgcaatat ggccgctccc
     2401 cgcgtgtcga tgtccaggat gacatcctgc ccggcggcaa agtatcgctc caggagcgcc
     2461 cggctcgtcc cgtagaggtg cccgtggacc acggcccact cggcaaactc ggcccggtcc
     2521 accatggcct ggaacactgc cggatccgcg aagtgatagt cgtggccgtc cctttcgtcg
     2581 ggccgaggag gccgggtggt gtaggagacc gagtgcgcca ggcccggcac cgccttgatc
     2641 acccactcgc agagtgaggt cttcccggcc ccggacggcg cggaaaccac gacgagcagc
     2701 gggcccgccc ttccccactc gggcaggtcg gcttgccggt cggaggtctc aggtccgctc
     2761 acggacctca tccgaccctc ccacccgctg ggcgagcgtg ctggactgga tggccgagag
     2821 cactacgtgg ccgctgtcgg tcacgatcaa ggtctgcgtc cgcctgccac acgtcgcgtc
     2881 cacgagtctt cccgcctccc gcgcgtcgtc ccgcaggcgc ttcatgggct ccgagaagac
     2941 agccttggaa tccttggacg accgggcgct caagatcgcc acgatccggg atcgcaagac
     3001 cacgttgccg aagccgatgt tcaagacctc ggtagccacc gcgcccaccg agtccccctg
     3061 cactaccgcc gcgaggcctt cggcctcccc ccgccgggtg ccggagagat cctgccgccc
     3121 ctcggactct ccgcgggccc aggctactcg atgttctgaa cttgctcgcg gaggcgctcg
     3181 acgatgagct tcagccccac cacatcctgg gccaaggtcg cgtccccggc cttggatccc
     3241 atggtgttgg cctcgcggtg catctcctgg atgaggaagt cgagcctgcg accttgcggg
     3301 cccgcctgtg tcaaggcctt tccgaactgg atcaggtggc tccgcagacg gtcgcactcc
     3361 tcggccacgt cgccgcgctc cgccaggaaa gccacctctt gctccaatcg ggtgggatcc
     3421 gggaggtgac cctccaggag gtccacgacc cgctcgcgca gacgttcgcg ctgcgcgcgc
     3481 accagatctg gcgcccgcac cacgatgccg tccaggatgg cctccacctc ttccaggtgc
     3541 ttcgcgatgg ccctgaccag agcctcgccc tcccggccac gcatctccgt cagggcatcc
     3601 agggcggcgg cgagcgcggg aaccacctca ccccaggtct cgtccaggga atcctcggat
     3661 tcctcgacgg cgatcagttc gtgctgggcg gcgagcatct ccagcgtcac ctcgccgggc
     3721 aactgaagtt cccgctgcaa ggtgcggagg gcgtccacgt actgcagtgc cagcgggacg
     3781 tccacccgga gcgtccggct acggccttcc aggtccttgt ccagcaccga aacttcaaaa
     3841 tgaccccggg cgaaccgctg ctggactgtc tgctggatcc ggggctccag gccggtcaga
     3901 cgcttcggca cccggcagcg cacctccagg aagcgatggt tgaccgagtg aacctcgacc
     3961 gtgaagacgc ggtcgcgcct cgtggcctca cctcggccaa acccggtcat gctctgccgc
     4021 acgcccgccg tctcggtccc cgtagaaacc tcccaagtcc ttggaatgat ttaaccagtt
     4081 accctaacct ggtcagcctc gaaagtcaaa tatcttgaca atttgggtct tctcgcctat
     4141 ggttctaccg gtctcaaatc tgctgccagg agggtcagtg acgtgccgat ctatgagtac
     4201 gagtgcgaat cctgcgacaa tcgattcgaa ctgatgcaga aattcagcga taaacccctg
     4261 aagaaatgtg tgaaatgtgg cggccccgtc cacaaggtcc tgtccgctcc cgcgctgctc
     4321 ttcaagggaa ccggctggta cgtaacggat tacgccaatc ccgaccggaa gagggcccag
     4381 aaggcggaca aggataacgg cggcaacggc gacaagaagg ccgagccggc agcctcgaaa
     4441 gccaccgcga caaccaacaa aagcaacaac tgatcctggc tcaacccagc cggacgcccc
     4501 cggacgggac accgccgggg gcgctggctt ttccggacgg ctccccatcg ccccgcagca
     4561 tccagcagct ccccaggcca aaaagggccc ccaggctgtc ccagccgaca tccaccgcgg
     4621 atgcgccgcg gtgtggttcg aaaacctgat ggatctcatc tgcagtaccg aaggcggcgg
     4681 ccaacaggaa ggcccggacg gcggcctgtg gcctccagcc tgcctctccc catttcagcg
     4741 cgcggtacca cagccaagcc agaacgccga actcgaacat gtgcatcccc ttccggatcc
     4801 cggcgtgcag gaggctcagg ctcgcggcgg aggcgccggg gatcacccag tgcaggaggg
     4861 ggatcaggta tcgactggtt tgctgcgagg agaacccatc cgtcgagagc aggctggcta
     4921 cccccatcca caggaaaggc ggaccccagc ggacgaggag ccacccgatg gtcctccccc
     4981 ggaagaccga ccggctcatg tggcccgacc cagcaggctt cgaacgcggg ctacggcctc
     5041 cgccggggcg accagactct cctcgcccgt acggcggcgg cggacctcga tcaggccctc
     5101 cttgatggcc cgacttccca cggtgacccg aagcggcacg cccaagaggt cggcgtcctt
     5161 gaacttcacg ccgggccgct cgtctcgatc gtcgtagagc acgtccaatc ccgccgcggt
     5221 cagatcccgg tagagcaatt ccgccagctc ccccatcttc tgatccttgg tgttgaccac
     5281 gagcagatgg acctcaaacg gcgcaatgct ctcgggccag atcatcccgt accgatcgtg
     5341 gttctgctcg atggccgcgg cagcgatcct ggccgggccg atgccgtaac tccccatgac
     5401 gatgggttgc tcatgcccct gctcatccaa atagaccgcc cggagcggga tcgagtactt
     5461 ggttccaagc ctgaagatgt tcccgatctc gatcaccgac tcaacctgga ggggggcccc
     5521 gcagcgtccg cagccgtccc ctgccttggc ctcatgcagg tcggcgaact cggcctggaa
     5581 gtctcgaccg gcgaccacgc cgcgaagatg ggtgtccggc ttgttggcgc cggccacgta
     5641 gcagccggtg cggaggcagg cgtcggcgat ggtccggagc gagaggccca cgggtcccac
     5701 gaaaccggcc tccccccccg tggcctgctc gatctcctcc tggtgggcgg gccgcaaggg
     5761 tcccagaatc cgctgcagct tccgctcgtg gagttgctgg tcgccccgga cgagcgccag
     5821 cgccgggccc ccctctccca ccaccacgag ggatttgatg gtgagggccg gctcgatctg
     5881 aaggagtcgg gacacctcgg cgatggtgcg ggcgttgggc gtggacactt cttccaggct
     5941 ccaattgggg aacgccggaa gcggcggccg ggacgcggcc agctccacgt tggcggcgta
     6001 ctgacaactg ggacacaggg ccacccgatc ttcccccgcc tggctcggcg ccatgaattc
     6061 gtcggagccg gcgccgccca tcatgcccgg atcgctttcc accacgacgt acttcacgcc
     6121 gcagcgatca tagatcctgc agtaggcttt gcggtgggcc tcgtactgaa cctccaggcc
     6181 cgccatgtcc cgatccaggc tgtaggagtc cttcatgacg aactcccgcg tccgcaggac
     6241 gccgcttttc ggccgggctt catcccgctc cttggtctgg atctgatacc aaagctgcgg
     6301 caggtcgcgg taggagtgga tctcccgcgc cgcgagccag gcgatgacct cctcgtgggt
     6361 catccccagg cacatgtctc gctggttccg gtccttcagc cggaacatct cgctgccgac
     6421 ggtgtcccag cggccggact gctgccagat ctccgccggc aggatcaccg gcatggtgat
     6481 ctcctggccg ccgatggcgt tgatctcctc ccggatgatg gcgttgatct tgtccatcac
     6541 gcgttgcccc aggggaaggt acacgtaaat cccggcggcc agctgccgca cgaagcccgc
     6601 ccggatcatc aacttgtggc tgaccgcctc ggcatcggcc ggatcttcct tccgggtcgg
     6661 aatcagcgac cgtgaccagc gcatggtggc gtcctctcct cccgtggaaa cacgaaggag
     6721 gacgcagaac gcgtccccct catcccttcg gccgcggccc gcgggcgcga cctactcctc
     6781 cgtcctggcg gccagcgcct tgacttcctc gatcaggaca tcggccaact ccgactcctt
     6841 caccttgcgg acgatctggc cattcttgat caggatcccc acaccgttgc cgccagcgat
     6901 gccgatgtcg gccccgcggg cctcgcccgg accgttcacg gcgcaaccca tcaccgcgac
     6961 gttcaagggg cggtggatgt tgaggacctc caggcgcttc tcgacctctt tcgtcagccc
     7021 caccaggtcg atctccaccc ggccgcagga ggggcaggcg atgacgttca gcccccgctt
     7081 ccggagcccc aaggccttca ggatctcgaa gccggcccgg atctcctcgg tggggtcctc
     7141 ggtgagactc acccggatgg tgtcgccgat cccctccgcc aggagcgtgc cgatccccac
     7201 cgccgacttg atcgccccgg tgaaagccgt gcccgcctcg gtcaccccca ggtggaacgg
     7261 gtagggaacc ttgtccgcca agagacgata ggccgcgatc atcattttgg ggtcggagga
     7321 tttgagtgag atcttcatct ctggatagtt caattcctcc aggatccgga cgtggaacag
     7381 ggcgctctcc accatggcct cgggcgtggg ctcgccgaag cgttgcagga ggtgcttctc
     7441 cagcgagccc gcgttcacgc cgatccggat cggcaccctg cgctccgccg ccgccttggt
     7501 cacctcccgc acgcgccagg tggctccgat gtttccgggg ttgatccgga ggcaatccac
     7561 gcccccctcc aggcactgga gggcga
//
LOCUS       DBNGZC010001059         5367 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1655760, whole genome shotgun sequence.
ACCESSION   DBNGZC010001059 DBNGZC010000000
VERSION     DBNGZC010001059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5367)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5367)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5367
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1655760"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="AC16G0_18290"
     CDS             <1..188
                     /locus_tag="AC16G0_18290"
                     /inference="COORDINATES: protein motif:HMM:NF012355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR5101610.1"
                     /translation="GTTVTWTNQDDMVHTVTSAAKVFSSASLETDDTFTYTFTTPGTY
                     TYFCKLHPHMTATVIVQ"
     gene            220..1194
                     /locus_tag="AC16G0_18295"
     CDS             220..1194
                     /locus_tag="AC16G0_18295"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR5101611.1"
                     /translation="MNEQLLKHQHNRIQTDPSQDGIDRRGFLECMAWVGTGLLWTVSG
                     GVLSSQRLTPALAAEMPRGEFHFAQISDSHIGFNADANRDVTGTLQQAIAKLNALQPA
                     PAFLLHTGDLTHGQKPSEFDALAENLKGASAKQVFYAPGEHDVFADGGKEFLTRHGKG
                     MAGNGWQSFDYQGVHFIGLNNVADLKAGGMGSLGKDQLEWLEKDVEGQASSTPLVVFA
                     HIPLWAVYPQWGWGTDDSAQALSYLKRFGSVTVLNGHIHQILQKVEGNITFHTAMSTA
                     FPQPAPGSAPSPGPMKVPAEELRHLLGIREVNVVPGKDALAVIDSRLA"
     gene            complement(1340..1642)
                     /locus_tag="AC16G0_18300"
     CDS             complement(1340..1642)
                     /locus_tag="AC16G0_18300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101612.1"
                     /translation="MQGTPDDAIPLLTKEEAIAEVRRWLEVEKVDPEKIFADTGDTTV
                     RYKDLIAHLERETPDGMLLLFAISRGRLIKKTREQEMQALLDIAIPPPKPEGPAPS"
     gene            complement(1685..1996)
                     /locus_tag="AC16G0_18305"
     CDS             complement(1685..1996)
                     /locus_tag="AC16G0_18305"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5101613.1"
                     /translation="MGQELVDVHQHRGRFFEVLQQTERSQTAVMTIAPGQDAGPEESH
                     PGDQVIYVIEGEVLIRLSGTEQKAAAGSLLTIPAGTPHHVRNPGRAPLFFVTVYAPPV
                     Y"
     gene            2129..2368
                     /locus_tag="AC16G0_18310"
     CDS             2129..2368
                     /locus_tag="AC16G0_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101614.1"
                     /translation="MPTQQELEEEDRRVRQLRLVVDLALSVIGQTNMTLTQAEEMVSA
                     TRGLALSLFPGKELAFDLIYLPRFRRLLTERYRLH"
     gene            2460..3368
                     /locus_tag="AC16G0_18315"
     CDS             2460..3368
                     /locus_tag="AC16G0_18315"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HIR5101615.1"
                     /translation="MSIAGGMDQAPLRGRQAGCDTIQVFTKSNRQWKAKPLAPREVEA
                     FKANLVSTGIRPVVAHGCYLVNLAAPRALVWKKSVAAFREEMERAERLGIPYLVTHPG
                     AHLGAGEAEGIARLAEALNVLHAALPGYAVQVLLETTAGQGSSLGYRFEQLAAILAKV
                     DRADRVGICLDTCHLFAAGYDLRSADGYRRTMRELAAILGIARVKAIHLNDSQKGLGS
                     RVDRHEHIGEGTLGLSAFRLVLNDARFRRVPMILETPKDPDFVTADRRNLARLRALLN
                     GRGQRGGGILRATVPVATRRSNRRLL"
     gene            3365..4369
                     /locus_tag="AC16G0_18320"
     CDS             3365..4369
                     /locus_tag="AC16G0_18320"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR5101616.1"
                     /translation="MTNPRILRVHPLAGVEGGRATIFGEGLCEPGAELPSPHFEAIES
                     RPLIASTTRIVAPIPEGAVSGFIRVVWPDAVAQGPFFEVAAKLADELHPVANPVVDRE
                     GYIITTFSGSRGQAAPSGVSVFRIPSDGGAEPLVNDLVNPTGLALDADGTLFISCRND
                     GTIRRLTPGGQVETFADGMGTATGIAFDQAGTLFVGDRAGGIFAVDRLGAVRRFVKLP
                     ASVAAYHLTFGPDGNLYVTAPTLSNDDPIFRVNPSGTVDCFFEHLARPQGLAFDRDGN
                     LFVVAIHRGERGIFKVSSDGKAELGVAGENLVGLTFDARGRMIIASTNAIYRLGWTGD
                     "
     gene            4561..>5367
                     /locus_tag="AC16G0_18325"
     CDS             4561..>5367
                     /locus_tag="AC16G0_18325"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5101617.1"
                     /translation="MAEMDDLIHDLRKRIAGEVRFDPTTKVLYSTDASIYEIEPLGVV
                     IPKTVEDVMAVVEVCGRYQVPTLPRGAGTALAGQSVGRAVHLDMSKYLNKILEINLEE
                     RWARIQPGVVLDELNAVLRPHDLWFAPDVSPSNRATLGGMMGNNSSGARSIIYGKTID
                     HVLEMRVLLADGEALTARALTDEELARKQDAPGTEGEIIRQIRRLTTTQREEILRRYP
                     KIMRRVGGYNLDAFVDGTAFNLARMLVGSEGTLAATLEAKINLVPRPRPTE"
BASE COUNT          914 a         1810 c         1728 g          915 t
ORIGIN      
        1 aaggcaccac cgtgacctgg accaaccagg atgacatggt gcataccgtg acctcggcgg
       61 cgaaggtctt ctcgtcggcg agtctggaga ccgacgacac cttcacgtac accttcacca
      121 ctcccggaac ctacacctac ttctgcaagc tccaccccca catgaccgcc acggtgatcg
      181 tgcagtaacc cagtggctgc ggccaccaag gaggacgata tgaacgagca gcttctcaag
      241 catcagcaca atcggatcca gaccgaccca agtcaggacg gcattgaccg ccggggattc
      301 ctcgagtgca tggcgtgggt cgggacagga ctcctctgga ccgtcagcgg gggagtcctc
      361 tcatcccaga ggctcacccc agccctggct gcggagatgc cgcgagggga gttccacttc
      421 gcccagatca gcgacagcca catcggcttc aacgcggacg ccaatcggga cgtgacgggg
      481 accctgcagc aggccattgc caagctcaat gcccttcagc cggccccggc cttcctgctc
      541 cacaccgggg atctcaccca cggccagaag ccctccgagt tcgatgcctt ggccgagaac
      601 ctaaaggggg ccagcgccaa gcaggtgttc tatgcccccg gggagcatga cgtcttcgcc
      661 gatgggggga aggagttcct gactcgccac gggaaaggga tggcgggaaa cggctggcag
      721 agcttcgact accagggggt ccacttcatt ggcctgaaca atgtggcgga cttgaaggcc
      781 ggcggcatgg gcagcctggg caaagaccag ctcgaatggc tggagaaaga cgtagagggg
      841 caggccagca gcacgcccct cgtggtcttc gcccacattc cgctgtgggc tgtgtatccc
      901 cagtgggggt ggggcacgga cgatagcgcc caggccctct cctacctcaa gcggttcgga
      961 tcggtgacgg tcttgaacgg ccacatccac cagatcctgc agaaggtgga gggcaacatc
     1021 accttccata cggccatgtc gacggccttc ccccagccgg cccccggctc tgccccctcc
     1081 ccaggaccga tgaaggtgcc cgcggaggaa ctccgccacc tgctgggcat ccgtgaggtg
     1141 aatgtggttc cgggcaagga cgccctggcc gtcatcgatt cgcggttggc ctaacccctg
     1201 gaaggccatc ccgtcgcccc tcccgacccc gggcgccctc gaatccgtca gggattccgg
     1261 gcgcccggcc cacattccac ctcgggggat cagcctgctg aaggacggtc ttcgcggccg
     1321 gttggtgacg gcccctgagc tacgagggag ccggtccctc cggtttcggc gggggaatgg
     1381 cgatgtctag gagcgcctgc atctcctgtt cgcgcgtctt cttgatgagt ctcccccgcg
     1441 agatggcaaa gaggagaagc atcccgtccg gcgtttcgcg ctccaggtgg gcgatgagat
     1501 ccttgtagcg tacggtcgtg tcccccgtgt cggcgaagat cttctccggg tcgaccttct
     1561 ccacctccag ccaacgcctc acctcggcga tggcttcttc cttggtcaaa agggggatcg
     1621 cgtcatccgg cgtaccctgc atcggacttc tccttccgct tccgtcttgg cacgcggcgc
     1681 cccctcaata caccggtggg gcgtacactg tcacgaagaa gaggggcgcc cgtccggggt
     1741 tcctgacgtg gtggggcgtc ccggcgggga tcgtgaggag agagcccgcg gccgccttct
     1801 gctcggtccc ggagagccgt atcagcacct ctccctcgat gacgtagatc acctgatccc
     1861 ccgggtgcga ctcctccggc ccggcatcct ggccgggagc gatggtcatg acggcggtct
     1921 ggcttcgctc cgtctgttgc aggacctcga aaaaccgccc acgatgttga tggacgtcca
     1981 ccagttcctg gcccatgccc ccacctcctc tcgacacgca ttgtatgagg cctaccgccg
     2041 gaccacaagg ggccatcccc catcgccgct cggtcttgga aaccgctgct tggcctgtta
     2101 tagtcctgca accgggaggg gacgtggagt gcccactcag caggagcttg aagaagaaga
     2161 ccgccgcgtg cgtcagcttc gcctcgtggt ggacctggcc ctgagtgtga tcggccagac
     2221 caatatgact ctcacccagg ccgaggagat ggtctccgcc actcgcgggt tggcgctcag
     2281 cctgtttcct ggcaaggaac tcgccttcga cctcatctat cttccacgct tccgccggct
     2341 gctgaccgag cggtacagat tgcattgagc ctggggtgat caaaacgaag cgagcccaag
     2401 accggacagg caaccgtccg tccgcggcga ggcgtccatg cgtccgcctc ggctctcaca
     2461 tgtcgatcgc cggggggatg gaccaggctc ccttgcgggg tcgtcaggcg ggctgtgaca
     2521 cgatccaggt cttcaccaag agcaaccggc agtggaaggc caagccgctg gctccgcggg
     2581 aggtcgaggc cttcaaggcc aacctcgtct ccaccggcat ccgcccggtg gtggcccacg
     2641 gctgctacct ggtcaatctg gccgccccgc gcgcccttgt ctggaagaag tcggtcgcgg
     2701 cctttcgcga ggagatggag cgggcggagc gtctcggcat tccctacctg gtcacccacc
     2761 ccggcgccca ccttggcgcc ggcgaggcgg aggggatcgc tcgcctggca gaggccctga
     2821 acgtcctgca tgcggcacta cccggctacg ccgtccaggt cctgctggaa accacggcgg
     2881 gccagggcag tagcctgggg taccgcttcg agcagctggc tgcgattctg gcgaaggttg
     2941 accgcgccga tcgggtgggg atctgcctgg atacctgcca cctcttcgcc gcgggctacg
     3001 acctccggtc cgcggacggt taccgccgga ccatgcggga gctggcggca atcctgggca
     3061 ttgcccgggt gaaggccatc cacctgaatg attcgcagaa gggcctcggc agccgggtgg
     3121 atcggcacga acatatcggc gaggggacgc tgggcctctc tgccttccgt ctggtcctga
     3181 acgatgcccg cttccggcgc gtgcccatga ttctggagac gcccaaggac cccgattttg
     3241 tcacggcgga tcggcgaaac ctggcgcggc tccgcgcctt gctgaacggc agggggcaaa
     3301 ggggcggcgg gattttgcgt gcgacggtcc cggtggcaac tcgacgttcc aacaggagac
     3361 ttctgtgacg aatccccgta tcctgcgcgt tcaccccctg gccggcgtcg agggtggccg
     3421 ggccacgatc ttcggcgagg ggctgtgcga acccggggct gaactcccct cgcctcactt
     3481 tgaggccatc gagtcccggc ccctgatcgc ctcgactacc aggatcgtcg cgcccatccc
     3541 cgaaggtgcc gtctctggat tcatccgcgt cgtgtggccc gacgcggtgg cccaggggcc
     3601 attcttcgag gtggcggcca agctggcgga cgaactccac ccggtggcga atccggtggt
     3661 ggaccgagaa gggtacatca tcaccacctt cagcggaagc cggggtcagg ccgccccgag
     3721 cggcgtctcc gtcttccgga tcccctccga cggcggggcg gaaccgctcg tgaacgacct
     3781 cgtcaatcca accggcctcg ccctggatgc cgacggtacg ctgttcatct cctgtcggaa
     3841 tgacggcacc attcgccggc tcactccggg aggacaggtc gagaccttcg ccgacgggat
     3901 gggcaccgcg accggcatcg ccttcgacca ggcggggacc ctcttcgtgg gcgaccgggc
     3961 cggcgggatc ttcgccgtgg accgactggg ggccgtccgc cggttcgtca agctcccggc
     4021 cagcgtggcg gcctaccatc tcaccttcgg accggatggc aacctctatg tcacggcgcc
     4081 caccctgtcc aacgacgatc ccatcttccg cgtcaaccca tcgggaacag tcgattgctt
     4141 cttcgagcac ctggcccgcc cccagggcct ggcctttgac cgggacggga acctcttcgt
     4201 ggtcgcaatc caccggggag agcgggggat cttcaaggtc tcctccgatg ggaaggcgga
     4261 gctgggtgta gccggcgaga atctggtcgg gctcaccttc gatgcccggg gccggatgat
     4321 catcgccagc accaacgcca tctaccggtt ggggtggacg ggcgactgat gctagatgat
     4381 cgatgatggt gggcacccat cgcgggccgc ccgcagcagg cggtgtgata ggtcccgcaa
     4441 ggctccgtaa agccgaacct cggaaggact atccgccttt gacgttgggc attgggattt
     4501 ggtcatttcc gaaaacattc gcgaacgagc tcagaaatat tcccggttga ggagtggccc
     4561 atggccgaga tggacgacct gattcacgac ctgcgcaagc gcattgccgg ggaggtgcgc
     4621 ttcgatccca caaccaaggt gctgtacagc accgatgcca gcatctacga gatcgagcct
     4681 ctgggcgtcg tgatccccaa gaccgtcgag gacgtcatgg ccgtggtcga ggtctgcggc
     4741 cggtaccagg tgccgaccct gccccgaggc gccgggaccg ccctggccgg ccagtccgtc
     4801 gggcgagccg tccacctgga catgtcgaag tacctgaaca agatcctgga aatcaatctg
     4861 gaggagcgct gggctcgcat ccagcccggg gtcgtcctgg acgagctgaa cgcggtcctc
     4921 cggccccacg acctctggtt tgcgcccgac gtctccccca gcaaccgcgc caccctgggc
     4981 ggcatgatgg gcaataactc ctcgggggcg cggtccatca tctacgggaa gaccatcgat
     5041 cacgttctgg agatgcgggt cctcctggcc gacggcgagg cgctcacggc ccgggccctg
     5101 accgacgagg agttggcgcg gaagcaggat gcgccgggta cggaggggga gatcatccga
     5161 cagatccggc gcctgaccac gacccagcgc gaggagatcc tgcggcgcta tcccaagatc
     5221 atgcgccgcg tgggcggcta caacctggac gccttcgtgg acggcacggc cttcaacctg
     5281 gctcggatgc tcgtgggctc cgaggggacc ctggccgcga ccctggaggc caagatcaat
     5341 ctggtgccgc gtcccaggcc caccgag
//
LOCUS       DBNGZC010001060         2694 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1362697, whole genome shotgun sequence.
ACCESSION   DBNGZC010001060 DBNGZC010000000
VERSION     DBNGZC010001060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1362697"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="AC16G0_18330"
     CDS             complement(<1..693)
                     /locus_tag="AC16G0_18330"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5101618.1"
                     /translation="MSQDRGKITRRELLKRAAIVSCVLPAAGSLLSAFPARVQAAAAG
                     PKKDLIFVEGTDITVLDPMMVTDTPSNCPNLLVYDGLVSYDKDMNIVPVLATKWSISP
                     DKKAWTFSLRPNVKFSNGDPFTSKSVQFTFERMMDQSTAAPSRSQYLAVERVETPDDL
                     TARFVTKYPFPDLLRNLAQPNLLAYNPAHTKKYSVQEYARNPLGTGPFMLKEWVSGDR
                     VVFVPNPHYWGGP"
     gene            complement(737..1708)
                     /locus_tag="AC16G0_18335"
     CDS             complement(737..1708)
                     /locus_tag="AC16G0_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5101619.1"
                     /translation="MSSPATGEHLLSGQPDRGPALRSSGSTENDRVRANGAPGTSPAG
                     SKHSWSRLRQFRRNRGGVAGFFVVVNFALVAVLANEIVPFNPVEQNLSVALQGPGESH
                     WLGTDELGRDILSRIIMGARVSLLMSVAAVIAASALGTLVGSAAGYFGGGLDHVAMRC
                     IDVLLALPDILLAITIIAVLGVGLINVVVAIAISGVPVFARLARAVTLTVKENEYVQA
                     ARAMGAADGRILARHVLPNIVPPLLVQCTLLMATAILTASSLSFLGLGAQPPTPEWGA
                     MLSSGRSYLTSSPHVGTFPGIALMLVVLGFNMLGDGLRDALDPRLKR"
     gene            complement(1753..2673)
                     /gene="nikB"
                     /locus_tag="AC16G0_18340"
     CDS             complement(1753..2673)
                     /gene="nikB"
                     /locus_tag="AC16G0_18340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011228608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease"
                     /protein_id="HIR5101620.1"
                     /translation="MSRYLLHRLLALIPVLFGVSLIVFSILKFVPGDPALQVGGLDAS
                     PEDLAAIRHQMGLDRPIHVQYLYFVGNAMRGDFGRSIRSHRPVTEELWSRLPNTFVLT
                     VAAMIIAIIAGTGLGILAATRQYTLWDNVSMALAMLGISVPIFWLGLMLMLLFALQLR
                     WFPTAGTGTPWHLVLPSLTLGTASSAIIARQVRSAMLEVLRQDYVRTAQAKGLRPRAV
                     VLRHALKNAMIPAVTIIGLQFGYLLAGAVVTETVFAWPGVGRLLVDAIKYRDFPVVQA
                     TILWLAVIFSTVNLAVDLVYGYLDPRIKYR"
BASE COUNT          513 a          865 c          884 g          432 t
ORIGIN      
        1 cggtcccccc cagtagtgcg ggttgggcac gaagaccacc cggtcgcccg acacccactc
       61 cttcagcatg aagggacccg tgcccagagg attacgggcg tactcctgga ccgagtactt
      121 cttcgtatgg gcggggttgt aggccaggag gttgggctgg gcgagattcc gcagcaggtc
      181 gggaaagggg tacttggtca cgaacctggc ggtgagatcg tccggggtct ccacccgctc
      241 cacggccagg tattgcgagc ggctgggggc ggccgtggac tgatccatca tccgctcgaa
      301 cgtgaactgc accgacttgg aggtgaaggg atcgccgttg gagaacttca cgtttggccg
      361 cagggagaag gtccaggcct tcttgtccgg actgatgctc cacttggtcg ccaggaccgg
      421 gacgatgttc atgtccttgt cgtagctgac caggccgtca tagaccaaga ggtttggaca
      481 gttgcttggc gtgtccgtca ccatcatggg atccagcacg gtgatatcgg tcccctccac
      541 gaagatcaga tccttcttcg gtcctgcggc cgcagcctgg actcgagccg ggaatgccga
      601 cagcaggctt ccagcagccg gcaggacgca ggacacaatg gctgctcgct tcaaaagctc
      661 ccttcgggtg atcttgcctc tgtcttggct catcttcccc tccttctcgt cgacttcgag
      721 aatgctcccc tggcgttcac cgcttgagtc tcggatcgag ggcgtcgcgc aggccgtcgc
      781 ccaacatgtt gaaccccagc accaccagca tgagggcgat ccccgggaag gtcccaacgt
      841 ggggactgct ggtgaggtaa gaccggccgc tgctgagcat ggccccccac tcgggcgtcg
      901 gcggctgcgc gcccagccca aggaaagaga ggctggaggc cgtgaggatc gccgtggcca
      961 tcaggagcgt gcactggacc aagagcggcg gcacgatgtt gggcaggacg tggcgcgcca
     1021 ggatgcgtcc gtcggcggca cccatggccc tggcggcctg cacatactca ttctccttca
     1081 ccgtcagggt gacggcgcga gcgagccgcg caaacacggg aaccccgctg atcgcgatgg
     1141 ccaccaccac gttgatcagg ccgaccccca gcacggcaat gatggtgatc gccaggagaa
     1201 tgtccggcag cgccagcagg acatcgatgc agcgcatcgc cacgtgatcc agcccgcccc
     1261 cgaagtaccc ggccgccgaa cccaccagcg tgcccagcgc cgaggcggcg atgacggctg
     1321 cgacactcat gaggagtgac accctggcgc ccatgatgat gcggctgaga atgtcgcgcc
     1381 ccagctcatc ggtccccagc cagtggcttt cccccgggcc ctggagggcc accgagaggt
     1441 tctgctccac ggggttgaag gggacgatct cgttggccag gacggctacc agggcgaagt
     1501 tcaccacgac gaagaagccc gccacgccgc cccggttccg ccggaactgc cgtagcctcg
     1561 accacgaatg cttcgaccct gccggtgacg ttcccggcgc accgttcgct cttacccggt
     1621 cgttttccgt ggatccggat gaccgaagag cgggtccacg gtccggttgc ccggaaagga
     1681 ggtgctcgcc agtcgccggt gaggagatcc gaccgagtcc cgtctccggc gtctggggcg
     1741 aattcggctg gctcatctgt acttgatccg tggatccaga tagccgtaga ccaaatccac
     1801 ggcgaggttg accgtggaaa aaatgaccgc cagccagaga atggtcgcct gcacgaccgg
     1861 gaagtcccga tacttgatgg cgtccaccag cagccgtccc acacccggcc aggcgaaaac
     1921 cgtctccgtg accaccgcac ccgccaggag atacccgaac tggaggccga tgatggtgac
     1981 ggccgggatc atggcgttct tgagggcgtg tcgcagcacc accgcgcgag gacgcagccc
     2041 tttggcctgg gcggtgcgga cgtagtcctg ccggagcacc tccagcatgg cggagcgcac
     2101 ctgccgcgcg atgatggcgg aggaggcggt ccccagcgtg agagagggga ggacgagatg
     2161 ccacggcgtg cccgtccccg ccgtcgggaa ccagcggagc tgcaaggcga agaggagcat
     2221 caacatgagg ccgagccaga atatcggaac cgagataccc agcattgcca aggccatgct
     2281 gacgttgtcc cacagcgtgt actgccgggt tgcggcgagg atgccgaggc cggttccagc
     2341 aatgatcgcg atgatcatgg ctgcgacggt cagcacgaag gtgttcggca gccgcgacca
     2401 cagctcctcc gtgaccgggc gatggctccg gatcgaccgg ccgaagtccc cccgcatggc
     2461 attgcccacg aagtagagat actgcacgtg gatcggccgg tccagaccca tctgatggcg
     2521 gatggccgcc agatcctcgg gcgaggcgtc cagaccccct acctggagcg ccggatcacc
     2581 cgggacgaac ttcaggatcg agaagacgat gagcgagacg ccgaacagga cgggaatgag
     2641 agccagcaac ctgtggagaa ggtaacgact catcgttatg gccgcctcga cgtt
//
LOCUS       DBNGZC010001061         1409 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1955072, whole genome shotgun sequence.
ACCESSION   DBNGZC010001061 DBNGZC010000000
VERSION     DBNGZC010001061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1409)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1955072"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1281)
                     /locus_tag="AC16G0_18345"
     CDS             complement(85..1281)
                     /locus_tag="AC16G0_18345"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5101621.1"
                     /translation="MHRLRSPGLWTLAVLSLSLAQGWEAAGASLTLGEINPLTGRFAA
                     QGNAVHRGIQLAVEEAAAHGNSAVSLITRDDEGKPDRAVAAAEELTTRQRVAALVGGY
                     VDTLVGPISEIAERRRVPYVATASLDERLTQRGYRYFFRISHLQGYVESMVGVVQDLF
                     RPQSVAILYSGTPGASQLALRQRERLQQAAIRVPVFEMFASGIADFTPLLGRVRHAGV
                     DVLIADAFFADHLVLVRQLHGLAVNVKAFLGAFGMEFPEVTRDLGPSSEFLYGTTGWE
                     PGIAEPGTDAASRSFVERYRSRFGGEPPPLAMHGYVAARAVLGAAERTERRTEPIDAE
                     SIRRGLLQLDLPTPLERLQFDERGEARHYRRLIVQIQEGSRVVVYPPDRATGKARYPM
                     PAWNNR"
BASE COUNT          234 a          488 c          471 g          216 t
ORIGIN      
        1 gccacccacg gccgcctcaa gcacggtgtc cctctccccg cggtccgggg ctcgcaaggc
       61 atcgttggcg atccgccgtg caggtcatcg gttattccaa gccggcatcg gatagcgtgc
      121 ctttcccgtg gcacgatccg gaggatacac cacgacgcgc gatccctcct ggatctggac
      181 gatgagacgg cggtaatgcc tggcctcgcc gcgctcgtca aactgcaacc gctccagcgg
      241 ggtgggcagg tccagttgga gcagaccccg ccggatcgac tcggcgtcga tgggttctgt
      301 ccggcgttcc gttcgctcag ccgctcccag gacggcccgg gcggcgacgt agccgtgcat
      361 ggccagggga ggcggttcgc ccccgaaacg ggaacggtac cgctcaacaa aggagcggga
      421 ggcggcatcg gtcccgggct cggcgatccc cggctcccaa ccggtggtcc cgtacaggaa
      481 ctcgctggaa gggccgagat ccctcgtgac ctcggggaat tccatcccga aggcccccag
      541 gaatgccttg acgttcaccg cgagcccgtg gagctgccgc accaggacaa gatggtcggc
      601 aaagaatgca tcggcgatga ggacatccac gcccgcatgg cgcacgcgac ccagaagcgg
      661 ggtgaagtcg gcgatgcccg aggcaaacat ctcgaagacc ggcacgcgga tggccgcctg
      721 ctgcagccgc tcgcgctggc ggagcgcgag ctgggatgcc cccggtgtgc cggagtagag
      781 aatggccacg ctctgcggtc ggaacagatc ctgaaccact ccgaccatgc tctccacgta
      841 gccctgcagg tgactgatgc ggaagaagta tcggtatccc cgctgggtga gccgctcatc
      901 cagggatgcc gtggccacgt aggggacacg acggcgctcc gcaatctcgc tgatcgggcc
      961 gaccagggta tccacatacc ctcccaccag ggcggcgacc cgctggcggg tggtcagctc
     1021 ttcggcggcg gccaccgcgc ggtccggctt cccctcgtcg tctcgggtga tcaagctgac
     1081 ggcggagttt ccgtgcgccg ccgcctcttc caccgccagc tggatgccgc ggtgcacggc
     1141 attcccctgc gccgcgaaac gccctgtcag gggattgatc tccccgagcg tcaggctggc
     1201 ccccgccgct tcccagcctt gtgccaagct caggctgagg acggcaagag tccagaggcc
     1261 gggtgaccga agccgatgca tgctctcctc gacgattcac ttcggggcgg gtctggggcg
     1321 caagccgatc tcgcacggca agtggaggag ccgccccgtc cgcttccatc gggtggacag
     1381 gatggactct cgcgggtcga gccccgacc
//
LOCUS       DBNGZC010001062         1598 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1855959, whole genome shotgun sequence.
ACCESSION   DBNGZC010001062 DBNGZC010000000
VERSION     DBNGZC010001062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1855959"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="AC16G0_18350"
     CDS             complement(<1..404)
                     /locus_tag="AC16G0_18350"
                     /inference="COORDINATES: protein motif:HMM:NF020977.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR5101622.1"
                     /translation="MPDQPGLGARLQAALNEARKRRDTARTLLLSTILADHKNREIEL
                     KRLPTDDESAEVLRRGVKRRREAAEQYTAGKRPELAAKELAEVAMLEEFLPQQASADE
                     IRAVVREAIAAGATDVGKVMGQVMPRLKGRADG"
     gene            complement(397..585)
                     /gene="rpsU"
                     /locus_tag="AC16G0_18355"
     CDS             complement(397..585)
                     /gene="rpsU"
                     /locus_tag="AC16G0_18355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003368017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HIR5101623.1"
                     /translation="MSEVIIHEDENFERALKRFKKKCEKAGILSDLRKHRHYEKPSER
                     RKRKMNAARRKNRRVRPA"
     gene            complement(628..966)
                     /locus_tag="AC16G0_18360"
     CDS             complement(628..966)
                     /locus_tag="AC16G0_18360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015257645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HIR5101624.1"
                     /translation="MGDCIFCKVANGEIPAAVIKRTEKFVAFQDINPQAPTHFLVIPT
                     YHVASLDQTKDGGLLGDLLATARDIARELGISNKGYRVVINTNPDGGQTVFHLHAHVL
                     GGRALQWPPG"
     gene            complement(970..>1598)
                     /locus_tag="AC16G0_18365"
     CDS             complement(970..>1598)
                     /locus_tag="AC16G0_18365"
                     /inference="COORDINATES: protein motif:HMM:NF013705.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HIR5101625.1"
                     /translation="GEVRRHAQSIFGQFLSVSPCPTCAGEGSVVQHPCETCHGDGRVK
                     AEKTIQIDVPAGVADHHYLTMRGQGVPGPRNGPAGDLIAVLEIQEDPRFERHGDDLLY
                     DLPVSFSQAALGEDVEVPTPYGPVTLSVQPGTQTGTIYRLRGKGLPRLGEGGRGDLHV
                     RTHVWTPSQLTKDQERLFREMKQVEGNPPSAESLGRSFWNKLKDALGA"
BASE COUNT          235 a          541 c          513 g          309 t
ORIGIN      
        1 ccgtccgccc gccccttcag gcggggcatg acctgtccca tgaccttgcc cacgtccgtg
       61 gcgccggcgg cgatcgcttc ccgcacgacg gcacgaattt cgtcggcgga cgcctgctgg
      121 gggaggaatt cctccagcat cgccacctcg gcgagctctt tcgccgccaa ctccggacgc
      181 ttcccggcgg tgtactgctc ggccgcttcg cgccggcgct tcacgccgcg gcgcagcacc
      241 tccgccgact cgtcgtcggt ggggaggcgc ttcagctcga tttcgcgatt cttgtggtct
      301 gcgaggatgg tggagagaag gagggtgcgg gcggtgtcgc gccgtttccg cgcctcgttg
      361 agcgcggcct gtagccgtgc cccgaggccg ggttgatcag gcagggcgca ccctccggtt
      421 cttacggcgc gccgcgttca tcttgcgctt gcgacgctca ctcggcttct cgtaatgacg
      481 gtgcttgcga aggtccgaca ggatgccggc cttctcgcac ttcttcttga agcgcttcag
      541 cgcccgctca aaattctcat cctcgtggat gatcacttcc gacatctcac acgctccatt
      601 taacctgcga agggggcgga agttagccta gccggggggc cattgcaatg cccgccctcc
      661 gagcacatgg gcatgcagat ggaacaccgt ctggcccccg tccgggttgg tattgatgac
      721 gacgcgatag cccttgttcg agatccccag ttcccgggcg atgtccctgg ccgtggccag
      781 gaggtccccc agcaaccctc catccttggt ctggtcgagc gacgcgacgt ggtaggtcgg
      841 gatcacgagg aaatgcgtgg gcgcctgggg gttgatgtcc tggaaggcca cgaacttctc
      901 ggtgcgtttg atcaccgcgg ccgggatttc gccgttcgcc actttgcaga agatgcagtc
      961 gcccatggct caggccccca atgcgtcctt cagcttgttc cagaagctcc ggcccaggga
     1021 ttcggccgac gggggattcc cctcgacctg cttcatctcc cggaacaggc gctcctggtc
     1081 cttggtgagc tgggaggggg tccagacgtg cgtgcgcacg tggaggtcgc cccgtccccc
     1141 ctcgcccagc cgggggagtc ccttcccccg caggcgataa atcgtccccg tctgcgtccc
     1201 cggttgcacg ctcagggtaa ctggcccgta gggagtcggg acctcgacgt cttcaccgag
     1261 cgccgcctga ctgaaggaca cgggaagatc gtagaggaga tcatcgccgt ggcgctcgaa
     1321 ccgcggatcc tcctggattt cgagcaccgc gatcaggtcg cccgcgggac cgttgcgggg
     1381 ccccggcact ccctgcccgc gcatcgtcag atagtgatga tcagccacgc cagccggcac
     1441 atcgatctgg atcgtcttct cggctttgac gcgaccatcg ccgtggcagg tctcacacgg
     1501 gtgctggacg acgcttccct caccggcgca ggtgggacag ggtgatacgg agaggaactg
     1561 cccgaatatc gattgcgcgt gccgccgtac ctcgccgg
//
LOCUS       DBNGZC010001063         3335 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2637351, whole genome shotgun sequence.
ACCESSION   DBNGZC010001063 DBNGZC010000000
VERSION     DBNGZC010001063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2637351"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="AC16G0_18370"
     CDS             complement(<1..138)
                     /locus_tag="AC16G0_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid kinase family protein"
                     /protein_id="HIR5101626.1"
                     /translation="MTLIVQKYGGTSVGDVDRITNVARRVTEARRAGQDVVVVVSAMA
                     GE"
     gene            complement(244..864)
                     /gene="plsY"
                     /locus_tag="AC16G0_18375"
     CDS             complement(244..864)
                     /gene="plsY"
                     /locus_tag="AC16G0_18375"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004620074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIR5101627.1"
                     /translation="MIRDVALVLGAYLLGSVPFGLLIARLRGGVDLRRVGSGNIGATN
                     VLRAMGTGAAALTLVGDIGKGALAVGIGRALEVSPALLAIIALSAVIGHLLPVFLGFR
                     GGKGVATTLGVVLAAMPVVGVLLLVVWIAVAIVWRYSSLAALVAAAALPVLAWGLDGR
                     PMMVVLAGVLFLLILLRHRENIARLCQGTEGKIGQKVAAPGSPEVT"
     gene            complement(992..1525)
                     /gene="pgsA"
                     /locus_tag="AC16G0_18380"
     CDS             complement(992..1525)
                     /gene="pgsA"
                     /locus_tag="AC16G0_18380"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR5101628.1"
                     /translation="MNLPNVLSLVRIFLVPVLVVFLIVVPRPYNLTAAVVFLAAVLTD
                     WLDGRIARSTRQVTTLGKLLDPMADKLLISASLISLVQVGRVQAWMVVLIVGRDLAIT
                     GLRGIAAAQNVIIQANDFGKATMIAEVIAVALLILNWPAISMSLGQAALVVAILLSLV
                     SGGVYFQKFWRSIDLGK"
     gene            complement(1544..2359)
                     /locus_tag="AC16G0_18385"
     CDS             complement(1544..2359)
                     /locus_tag="AC16G0_18385"
                     /inference="COORDINATES: protein motif:HMM:NF024793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeA family protein"
                     /protein_id="HIR5101629.1"
                     /translation="MSYEQFYHLREQPFSNTPDARFFFEVDAHSDVLARLMYAVNTMK
                     GLAVVIGDVGTGKTTLARRMLDQLDEDQYESALLVIIHSSISAEWLLRKIATQIGVDQ
                     VAEEKIAILTQIYHRLVELHQLGRKAVVLIDEANMLAQQPLMEELRGFLNLEVPGSKL
                     ITFILLGLPNLESNLALDAPLVQRVAVKCRLTSLKPETTKAYILHRLKVAGAAREIFS
                     DEVLNQIALDSKGIPRLINTICDNVLLEGFLRKREQIDVDIVTDVVKDLGLEA"
     gene            complement(2379..>3335)
                     /locus_tag="AC16G0_18390"
     CDS             complement(2379..>3335)
                     /locus_tag="AC16G0_18390"
                     /inference="COORDINATES: protein motif:HMM:NF024579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101630.1"
                     /translation="RLAAPAAGAAPETLEPLATPLASDAGTSVPREVTPSASLQDLVL
                     PEEPSLELEAETLGTPSEPQDDTAEQQPAGLPDEIEEAQVAPTAAELLATSGPAASVE
                     EFGAEFSLTETGGAAPAEGFVDVGAELENELAAPDQTAASPGGGPLMNGLLEEFQKGV
                     REHLDEKDFETHYNLGIAYKEMELYEEAVQEFRLAGRDPGRALACADLLGLCFVAMGQ
                     VDQAVLEYRAGLDIQGHPKEAYHPLRYNLGLAYETQGDFPHALEQFELLPAEDPRFHE
                     VREKLKSLRERMPRPVAAPGPSAPTPPAPAPRRAKEKKISFI"
BASE COUNT          603 a         1180 c         1007 g          545 t
ORIGIN      
        1 ctcgcctgcc atggccgaga caaccaccac cacgtcctgc cccgccctcc gggcctcggt
       61 cacgcggcgg gccacgttcg tgatgcggtc cacgtcaccc accgaggttc ccccgtactt
      121 ttgcacgatc agcgtcatcg ctcacctcgt tggtcgtgtc cagggtcgcc ggtattctcc
      181 cgccccttct ccgcctgcac ccctcgagtc gccgtccgtt catccggtcc tgcgagggtt
      241 cgatcaggtg acctccggac ttccgggagc agcaaccttc tgaccgatct tcccctcggt
      301 tccttgacac agacgggcga tgttttcccg atgccgcaac aggatgagga ggaagaggac
      361 cccggccagg accaccatca tcggccgccc gtcgagcccc caggccagga ccggcaatgc
      421 cgcggcggcc accagagccg ccagcgagga ataccgccag acgatcgcca ccgcgatcca
      481 gaccaccaag agcagcaccc ccacgaccgg catggccgcc aggaccacgc ccagggtcgt
      541 ggcgaccccc ttgccacccc ggaaacccag aaagaccggc agcaggtgcc cgatcaccgc
      601 cgacagagca atgatggcca ggagggccgg cgagacctcc agcgcacgac caatgccgac
      661 tgccagcgcc cccttgccga tgtcacccac cagggtgaga gccgccgccc ccgtccccat
      721 tgcgcggagc acgttcgtgg ccccgatgtt cccgcttccc acccgccgaa gatccacacc
      781 accccggagc ctggcgatca ggaggccgaa gggcaccgag ccgagcaggt acgcgcccag
      841 caccaaggcg acatcgcgga tcacagcccc accccacagg ccgacgatcc ggcactgcga
      901 ccatcaggca accgggtaat cccgtggagt cgagcccccg gttgtcccga ggtaccgagt
      961 agcccgccag ccgttttgcc cgggtgtccg gtcacttgcc cagatcgatc gaccgccaga
     1021 acttctggaa gtagacccca cctgacacga gggacaggag gatcgccacc acaagagccg
     1081 cttgccccag ggacatggag atcgccggcc agttcagaat cagcagggca acggcgatca
     1141 cctcggcaat catggtggcc ttgccgaagt cattggcctg gatgatgaca ttctgggccg
     1201 ccgcgatccc gcggagcccg gtgatggcca ggtcgcggcc cacaatcagc accaccatcc
     1261 acgcctggac ccgacccact tgcaccaggg agatcaggct ggccgagatc agcagcttgt
     1321 ccgccatggg atccagcaac ttccccaggg tggtgacctg gcgggtgctt cgggctatgc
     1381 gaccgtcgag ccagtcggtc aggaccgcag ccagaaacac caccgcggcc gtcagattgt
     1441 acggccgcgg gaccacgatc agaaagacca ccaggaccgg caccaggaag atccggacca
     1501 ggctgagcac gttgggaaga ttcacgccac cctcgcggcc cgcttacgcc tccagaccca
     1561 agtctttcac cacatccgtg acaatatcca catcgatctg ttcccgcttc cggaggaagc
     1621 cctccagcag gacgttgtcg cagatcgtgt tgatcagccg gggaataccc ttggagtcca
     1681 gagcgatctg gttcaagact tcatccgaga agatctcccg agcggcgccg gccaccttca
     1741 gacggtggag aatatacgcc ttggtggtct cgggcttgag tgaagtcaag cgacacttca
     1801 cggccacccg ctggaccagc ggggcatcca gcgccagatt ggactccagg tttgggagac
     1861 ctaagaggat gaaggtaatg agcttgcttc cgggcacctc caggttgagg aagccccgca
     1921 gctcctccat cagcggctgc tgcgcgagca tgttggcctc gtcgatcagc accaccgcct
     1981 tccgcccgag ctggtgcagt tccaccagcc ggtggtagat ctgagtcagg atggcgatct
     2041 tttcctcggc cacctggtcc accccgatct gcgtggcgat cttgcgcagc agccactccg
     2101 ccgagatgga ggagtggatg atcaccagga gtgcggactc gtattggtcc tcgtccagtt
     2161 ggtccagcat tcgtctggcc aaggtggtct tcccggtccc cacatccccg atcaccaccg
     2221 ccaggccctt catcgtgttc acggcataca tgagtctggc cagcacatcg gaatgggcgt
     2281 ccacctcgaa gaaaaatcgg gcatctggcg tattggagaa cggctgctcg cgtaggtggt
     2341 agaactgctc gtagctcatg ccggctttgc ctctggcgtc agatgaagga gatcttcttt
     2401 tccttggcgc gcctgggtgc cggcgcgggt ggggtcgggg cgcttggtcc aggcgccgcc
     2461 actggccgcg gcatccgctc tcggagcgac ttcagcttct cccgcacctc atggaatcgc
     2521 ggatcctcgg ccggcagcag ctcgaactgc tccagcgcgt gggggaagtc accttgggtc
     2581 tcgtaagcca gccccaggtt atagcggagg gggtggtagg cctccttcgg atgcccctgg
     2641 atgtctagtc ccgcccggta ctccaggacg gcctgatcca cctgtcccat ggccacgaaa
     2701 cacaacccga ggagatctgc gcacgccagg gcccgccccg gatcgcgacc cgccaggcgg
     2761 aactcttgga ccgcctcctc gtaaagttcc atctccttat acgcgatgcc caggttgtaa
     2821 tgcgtctcga agtccttctc atccaggtgc tcgcgcaccc ccttctggaa ctcctccagc
     2881 aggccgttca tcagcggccc gcctccggga gaggcggcag tctggtctgg agccgccagc
     2941 tcattctcca gctcggcacc cacgtcgaca aagccctcgg cgggagcggc accacccgtc
     3001 tccgtcagcg agaactctgc cccgaactcc tcgacggaag ctgcggggcc cgaagtcgcc
     3061 aagagctcgg cggcggttgg ggcgacctgg gcctcctcga tctcgtccgg aagcccggcg
     3121 ggctgctgct ccgcggtgtc gtcctggggc tcggagggag tccccagcgt ctctgcctcg
     3181 agttccaggg acggctcttc ggggagcacc aagtcctgaa ggctcgccga gggggtcacc
     3241 tcccgcggga cagacgtgcc ggcgtcggag gcaaggggtg tcgccaaggg ttccagggtc
     3301 tcaggcgccg caccggcggc tggggccgcc agccg
//
LOCUS       DBNGZC010001064         1830 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1066925, whole genome shotgun sequence.
ACCESSION   DBNGZC010001064 DBNGZC010000000
VERSION     DBNGZC010001064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1066925"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="AC16G0_18395"
     CDS             <1..335
                     /locus_tag="AC16G0_18395"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HIR5101631.1"
                     /translation="NVFFGIHDPTTLNRQGADAGTQYRSAIFYHDEEQKAIAEQLIQE
                     LNSQHIWDHPIVTQVVKFDKYYKAEDYHQEYYANNPFQPYCMAVVAPKVRKFRYHFME
                     MLKKPETA"
     gene            complement(620..1045)
                     /locus_tag="AC16G0_18400"
     CDS             complement(620..1045)
                     /locus_tag="AC16G0_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR5101632.1"
                     /translation="MPIESDNELREILKSTRTVASVGVSSNPDKPSYGIFQYLMEAGY
                     RMIPVNPTTPQILGRQTYPDVPSIPEKIDVVQVFRKPEDVPPVVEQAIKAGAKVVWMQ
                     EGVVNEEAAARAEKAGLKVVMDRCMRETHRRLFGGLTQL"
     gene            complement(1145..>1830)
                     /locus_tag="AC16G0_18405"
     CDS             complement(1145..>1830)
                     /locus_tag="AC16G0_18405"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5101633.1"
                     /translation="RALPEPWQLAPTLVSAGWDSAMLSGLEEDFPGPTLGPVLQWLVS
                     GLAVHFLLDEVAMGAERVSAIVKDVKAYAYLDQAPIQQVDIPESLEKTLAILRPKFDG
                     IQIVRDYALDLPRVEAYGGELNQVWTNILDNAAYAMQGTGTLQLRATRQNGEVLVEIQ
                     DNGPGIPPEIQQRIFEPFFTTKPPGQGTGLGLHIAYSIINNHYGRIRLTSQPGSTCFQ
                     VWLPIELPR"
BASE COUNT          343 a          564 c          570 g          353 t
ORIGIN      
        1 tgaacgtctt ctttggcatc cacgatccga ccaccctcaa ccgccagggc gcggacgctg
       61 gcacgcagta ccgctcggcc atcttctacc atgacgagga gcagaaggcg atcgccgagc
      121 agctcatcca ggagctcaac agccagcaca tctgggatca cccgatcgtg acccaggtcg
      181 tgaagttcga caagtactac aaggcggaag actatcacca ggagtactac gccaacaacc
      241 cgttccagcc ctactgcatg gcggtggtgg cacccaaggt gcgcaagttc cggtatcact
      301 tcatggagat gctgaagaag cccgagacgg cgtgagggcg ttcgaacgtt gaaatgctgg
      361 aacgttggaa agagagagca gcgagcggcg catgtgccgc tcgctgcttt tttgttcgaa
      421 tagctgtcag ctcaaacgcc ccaccccgtc cctccccaaa tgccagcaat tatgttggtt
      481 tgcaggcagg gggaatgatt actctgttgg aaatccaatc cgccttgagg gcatttgggg
      541 accccctccg ccacgctccg gggactcggg gtggctccgc accgccgaag gcgggcgggc
      601 cgggtagggc gtggtccgct cacaattgcg tcaggccgcc gaacaggcgg cggtgagtct
      661 cgcgcatgca gcggtccatg accaccttca gacccgcctt ctcggcccga gccgccgctt
      721 cctcgttcac gacgccctcc tgcatccaga ccactttggc gccagcctta atggcctgct
      781 ccaccaccgg cggcacatcc tccggcttgc ggaagacctg caccacgtcg atcttctccg
      841 ggatggatgg cacatccggg taggtctgcc tgccgaggat ctgtggcgtc gtagggttga
      901 cggggatcat tcggtagccc gcctccatca ggtattgaaa gatgccgtag ctgggtttat
      961 cgggattcga cgacacgccg acggatgcaa ccgttcgggt gctttttagg atctcccgca
     1021 gctcgttgtc agattcaata ggcatcatag actccagtgc aaagagggga tattgggtgt
     1081 cggaggtcga acgcccgaac ccggcctccg accgcctgcc ccgcccgtgg cggggttgac
     1141 ttttctacct tggaagctct atcggaagcc aaacctggaa acaggtgctt cccggctgtg
     1201 aggtgaggcg gatgcggccg taatgattgt tgatgatgct gtacgcgatg tgcaggccga
     1261 gaccggtgcc ctgccccggg ggcttggtcg taaagaaggg ctcgaagatg cgctgctgga
     1321 tctcgggggg aatgccggga ccgttgtcct gaatctccac caacacctcc ccgttctggc
     1381 gggttgcgcg caactggagt gtccctgtgc cctgcatggc gtaggccgcg ttgtccagga
     1441 tgttggtcca gacctggttc agctcgccgc cataggcttc cacgcgcggc aggtccaggg
     1501 cgtagtcccg gacgatctgg atgccgtcga acttggggcg gaggatcgcc agcgtcttct
     1561 ccaggctttc cgggatgtcc acctgctgga tgggcgcctg atcgaggtac gcataggcct
     1621 tgacgtcctt gacgatggcc gacactctct cagcgcccat tgctacttcg tcaagcagaa
     1681 aatggactgc caggccggac accagccatt ggagcaccgg gccgagggtt ggccccggga
     1741 aatcctcttc gagccccgag agcatcgcag aatcccaccc ggcggaaacg agcgtcggcg
     1801 ccagctgcca gggttcgggg agagcgcgct
//
LOCUS       DBNGZC010001065         3154 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2534624, whole genome shotgun sequence.
ACCESSION   DBNGZC010001065 DBNGZC010000000
VERSION     DBNGZC010001065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2534624"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="AC16G0_18410"
     CDS             <1..202
                     /locus_tag="AC16G0_18410"
                     /inference="COORDINATES: protein motif:HMM:NF015366.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HIR5101634.1"
                     /translation="VFPAGTPMEIVKYMEQSIKKAMEDPEHVKRMQEAGLTLKFMGIE
                     EYGKFLDSQNDRAKQLIGLYRK"
     gene            199..657
                     /locus_tag="AC16G0_18415"
     CDS             199..657
                     /locus_tag="AC16G0_18415"
                     /inference="COORDINATES: protein motif:HMM:NF018979.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HIR5101635.1"
                     /translation="MTAVGRVVWGILLVFGGFVVHESLDLSYYGSDFGPGPGFFSFWL
                     GVLLIVLSLIQVAGTFRGPRELLPEDFIPGADGIKRMLCILGALVASLLLMKPLGFSL
                     TMLAFCIFLFRTLGSQSWWLTLTLAVAGSFGLFYVFGLLQVILPTGLFGI"
     gene            671..2182
                     /locus_tag="AC16G0_18420"
     CDS             671..2182
                     /locus_tag="AC16G0_18420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012694953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HIR5101636.1"
                     /translation="MDAFQHLMMGFGVALSPMNLLFATIGCVLGTLIGVLPGLGPAAG
                     TALLIPITFKLDPTAAIIMLAAIYYGAMYGGTITSVLINVPGEAASVVTCLDGYEMAK
                     KGRAGAALGIAAIGSFIGGTVATFGLVLVALPLTRFALKFGPPEYFALLCVGLSLVMG
                     LAGKSLIKALTMGILGLLFSLVGVDPIEGAPRFTFGIAPLMDGIRFVPVVMGFFGIGE
                     ILINAEKSLVGVMAGEGKVTSFFPRRDELWISTTSILRGTGLGFFLGLIPGTNSAVAS
                     FMSYAVEKKVSRHPETFGTGVIEGVAAPETANNAYANAAMIPLFTLGLPGSPTIAILM
                     GALIMNGLTPGPLLFSQHAAFVWAVIASLYIGNVILLILNLPLVGWWAKLLRIPYNIL
                     VVFILVFCIIGGYSLNNSMFDVGVMILCGLLGYIFKKLEFPLAPAILTLILGPLMERS
                     LRESLSLSQGDYSIFITRPIALFFLIVTAVILTTSAWQAWPAAVREESKEAQV"
     gene            2405..>3154
                     /locus_tag="AC16G0_18425"
     CDS             2405..>3154
                     /locus_tag="AC16G0_18425"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012927147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR5101637.1"
                     /translation="MTDQGHLDQLCINTIRMLAADAVQKAKSGHPGLPMGTAAMAYVL
                     WTRFLKHNPANPKWPDRDRFILSAGHGCLLQYSLLHLTGYDLSLEDLQSFRQWGSRTP
                     GHPEYSLTPGIETSTGPLGQGVGNGIGMAIAEAWLAAQFNRQGHTIVDHTTYAIVSDG
                     DLMEGVASEAASLAGHLKLGKLIYLYDNNHISIDGSTDLAFTEDVGRRFEAYGWHVQR
                     VADGNDLAAVEAAIRAGKAERSRPSIILARTH"
BASE COUNT          524 a          996 c         1001 g          633 t
ORIGIN      
        1 tgtgttcccg gctgggaccc ccatggagat cgtgaagtac atggaacaat ccatcaagaa
       61 ggcgatggaa gacccggaac acgtgaagcg gatgcaggag gcaggactca ccctcaagtt
      121 catgggcatt gaggagtacg ggaagttcct cgactcccag aacgatcggg cgaagcagct
      181 catcggcctg taccgcaaat gacagcggtc ggccgggtcg tctggggaat cctgctggtc
      241 tttggcggat tcgtcgtcca cgagtcgctg gacctcagct actacggcag cgacttcggg
      301 ccgggacccg gcttcttctc gttctggctt ggggtcctgc tgatcgtctt gtccctcatc
      361 caggttgccg ggaccttccg cggaccgcgg gaactcctgc cagaggactt catcccgggt
      421 gcggatggca tcaagcgcat gctgtgcatc ctcggggctc tggtggcgtc cctgctcctg
      481 atgaaaccct tgggcttcag cctgaccatg ctggctttct gcatcttcct gttcaggaca
      541 ctggggagtc agtcctggtg gctcacgctg accctggccg tggcgggcag cttcggcctg
      601 ttctacgtgt ttggactgtt gcaggtgata ctgcccacgg gactcttcgg catctaaagg
      661 gggggagcgc ttggatgcct ttcaacactt gatgatgggt ttcggggtgg cactctcccc
      721 gatgaacctg ctcttcgcca caatcggctg cgtgctggga accctgatcg gtgttctgcc
      781 cgggctggga ccggcggcag ggacggcgct cctcattccc atcacgttca agctggatcc
      841 gacggcggcc atcatcatgc tggcggccat ctactacggg gccatgtacg gggggaccat
      901 cacctcggtc ctcatcaacg tccccggcga ggcggccagc gtggtgacgt gcctggacgg
      961 ctatgagatg gccaagaagg ggcgggccgg agcagccctg ggaatcgcgg ccatcggctc
     1021 tttcatcggc ggcacggtgg ccaccttcgg cctggtgctg gtggcgctac cgctcacgcg
     1081 cttcgccttg aagttcgggc ccccggagta tttcgccctc ctctgcgtgg gtctctccct
     1141 ggtcatgggt ctggccggaa agtccctgat caaggccctg acgatgggga ttcttggcct
     1201 cctcttctcc ctcgtgggcg tcgatcccat tgaaggcgcc ccgcgcttca ccttcggcat
     1261 cgcgcccctg atggatggaa tccggttcgt tccggtggtc atgggcttct tcggcattgg
     1321 ggagatctta attaatgcgg agaaatccct ggtgggggtc atggcgggcg agggaaaggt
     1381 cacctcgttc ttcccccggc gggacgagtt gtggatcagc acgacgtcca ttctgcgcgg
     1441 caccggtctg ggattcttcc tgggcctgat cccgggtacg aactctgccg tggcgtcgtt
     1501 catgtcctat gctgtcgaga agaaggtttc aaggcatccg gagacgttcg ggacgggcgt
     1561 gatcgagggc gtggcggcgc cggagacggc caacaacgcc tacgcgaatg ccgccatgat
     1621 cccgctcttc accctgggac tgccgggctc gcccacgatc gccatcctga tgggcgccct
     1681 gatcatgaac ggcttgacgc cggggccgct cttgttctcg cagcacgccg ccttcgtctg
     1741 ggcggtcatc gccagcctgt acatcgggaa tgtgattctg ctgatcctga acctgcccct
     1801 cgtcggctgg tgggcaaaat tgctgaggat tccttataac atcctggtgg tgttcatcct
     1861 cgtcttctgc atcatcgggg gttacagcct gaacaacagc atgttcgatg tcggggtgat
     1921 gatcctctgc gggctcctgg ggtacatctt caagaaactg gagttccctt tggcgccggc
     1981 gatcctcacg ctgatcttgg gccccctgat ggagcgaagt ctgcgcgagt ccctgtccct
     2041 ctcccagggg gattacagca tctttatcac acggcccatc gcgctgtttt tcctgatcgt
     2101 gacggcggtg atcctgacga cctccgcttg gcaggcctgg cccgctgccg tgcgcgagga
     2161 gagcaaggaa gcgcaggtct agccgcccga cacggcttgt tccgatggtc cagcccgacg
     2221 atccgccgcc ggaggtcgta tctcgactgc gccctcgggc ggcgacgtcc ttgccagatc
     2281 cactcccgca cagccacatc cctcacgcga cacacacctg actgacaact gatgccctgg
     2341 tatgctaggg tataaaaacc tggcgtggac cggtgaatca cctgccttga gtagggaggc
     2401 tcgcatgaca gaccaggggc atctcgatca actctgcatc aacacgattc gcatgctcgc
     2461 cgcggatgcc gtccagaagg cgaagtcggg ccatccgggc ttgcccatgg ggacggccgc
     2521 catggcctac gtcctgtgga cgcgattcct gaagcataat cccgccaacc ccaagtggcc
     2581 cgaccgggac cgcttcatcc tctcggccgg tcacggctgc ttgctgcagt acagcctgct
     2641 gcacctgacc ggttacgacc tgagcttgga ggacctccag agcttccgcc agtggggcag
     2701 ccggacgccg ggccacccgg agtacagcct cacacctggg atcgagacga gcacgggccc
     2761 cctggggcag ggcgtgggca acgggatcgg gatggccatc gccgaggcct ggctggcggc
     2821 gcaattcaac cggcaagggc acaccatcgt ggatcacacc acctacgcca tcgtcagcga
     2881 cggagacctg atggagggtg tggcgtccga ggccgcctcc ctggccggac acctgaagct
     2941 ggggaagctg atctacctgt atgacaacaa ccacatctcg atcgacggga gcaccgacct
     3001 ggccttcaca gaggacgtgg gccggcgctt tgaggcctac ggatggcacg tccagcgggt
     3061 cgccgacggc aacgacctgg ccgccgtgga ggcggcgatc cgtgctggga aggcggagag
     3121 gtcgcggccc tccatcatcc tggcccggac ccac
//
LOCUS       DBNGZC010001066         1916 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2345248, whole genome shotgun sequence.
ACCESSION   DBNGZC010001066 DBNGZC010000000
VERSION     DBNGZC010001066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2345248"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..667)
                     /locus_tag="AC16G0_18430"
     CDS             complement(<1..667)
                     /locus_tag="AC16G0_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR5101638.1"
                     /translation="MLSDRFYITTPIYYVNATPHLGHAYTTILADTVARYHRLAGRQV
                     YFLTGTDEHGDKIAQAAARATETPQAYADRISAAFRETWRRMALSFDQFIRTTEERHK
                     RVVAAVLQKIYDSGDIYFGEYGGYYCVRCERFYTEKEMVDGKCPQHQTPLTYVKEENY
                     FFRMSKYQDWLIRHIQEHPDFIRPERYRNEALGFLREPLEDLCISRPRARLEWGIPMP
                     FDDR"
     gene            complement(679..1686)
                     /locus_tag="AC16G0_18435"
     CDS             complement(679..1686)
                     /locus_tag="AC16G0_18435"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 domain-containing protein"
                     /protein_id="HIR5101639.1"
                     /translation="MTRLVRVEFGGSARYQYCVSPNDPLRPGEVVVVETDWGVGMGEV
                     RAVLGRISPEQMKTSVRPILRKATATDLSRRAALGQREREARAFCRERILARNLPMKL
                     TEVRIGLDSSKAMFYFTADGRVDFRELVKDLIQHLRTRVELRQIGVRDEAKVLGSSGP
                     CGRPVCCRAFLQAFAPVSIRMAKDQNLALNPGKLSGMCGRLKCCLAFEHSLYADLKRG
                     LPKVGSRVATCRGSGLVQGLNILDQTVQVGLETGGQVTLRPTEIQARPRHDTPQGPAG
                     GKTSGDQASDGREGAPAARRSQSKRSGDRRGKTSEPANHEPPAPENGDVSAEVKALEE
                     E"
     gene            complement(1689..>1916)
                     /locus_tag="AC16G0_18440"
     CDS             complement(1689..>1916)
                     /locus_tag="AC16G0_18440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101640.1"
                     /translation="VAVAKVGGSAVALLPPDRRTAAEQLAAAHSMGAILNIYAAQVEA
                     QWALARYANPRLTAERMLFRMRQAIGRGQRG"
BASE COUNT          297 a          654 c          616 g          349 t
ORIGIN      
        1 accgatcatc gaagggcatg gggatgcccc actccagacg ggcgcgcggg cgcgagatgc
       61 acaggtcctc caggggctcg cgcaggaacc ccagcgcctc gttccggtag cgctccggcc
      121 ggatgaagtc cggatgctcc tgaatgtggc ggatcagcca atcctggtac ttgctcatcc
      181 ggaagaagta attctcctct ttgacgtacg tgagcggcgt ctggtgttgg gggcacttgc
      241 cgtccaccat ctccttctcg gtgtagaagc gctcgcaccg gacgcagtag tagccgccgt
      301 actcgccgaa gtagatgtct ccgctgtcgt agatcttctg gaggacggcc gccacgaccc
      361 gcttgtgccg ctcctcggtg gtccggatga actgatcaaa gctgagggcc atgcgccgcc
      421 aggtctctcg gaaggcggcg ctgattcggt cggcgtacgc ctggggagtc tccgtcgccc
      481 gagcggccgc ctgagcgatc ttgtccccgt gctcgtccgt gccggtgagg aagtacacct
      541 gccgcccggc cagccgatga tagcgcgcca ccgtgtccgc caggatggtg gtgtaggcgt
      601 ggcccagatg cggcgtggcg ttcacatagt aaatgggagt ggtgatgtaa aatcgatcgc
      661 tcagcatgca gtctccattc attcctcctc cagggccttc acttccgccg aaacgtcgcc
      721 gttctcggga gcaggcggct cgtggttcgc aggctcggag gtcttccccc ggcggtcccc
      781 gctccgcttc gattggctgc ggcgtgctgc cggggcccct tccctcccgt ccgaggcctg
      841 gtccccggat gtcttccccc cggcgggccc ttggggcgta tcatgacggg ggcgagcctg
      901 aatctccgtg ggccggaggg taacctgtcc gcccgtttcg aggcccacct gaaccgtctg
      961 gtctaggatg ttcagcccct gcaccaggcc ggacccccgg catgtcgcga cgcggctgcc
     1021 caccttcggc agaccgcgtt tcagatcggc gtaaagcgag tgttcgaagg ctaggcagca
     1081 tttgagccgg ccgcacatcc cggagagctt gcccggattc agggccaggt tctgatcctt
     1141 ggccatccgg atgctcaccg gggcgaaggc ctggagaaag gcccggcagc agaccggccg
     1201 gccacacggc cccgaactgc ccagcacctt ggcctcatcg cgcaccccga tctgccgcaa
     1261 ttccacccgc gttcgcaggt gctggatgag gtccttcacc agctcccgaa agtccactcg
     1321 gccgtcggcc gtgaagtaga acatggcctt gctggaatcc aggccgatgc gcacctccgt
     1381 cagcttcatg gggagattgc gggctaggat gcgttcgcgg caaaaagcgc gcgcctctcg
     1441 ctcccgctga cccaaggcgg cgcgccggga caagtcggtg gctgtggcct tgcgcagaat
     1501 tgggcgaacg gacgttttca tctgctccgg agagatgcgc cccaggacgg cccggacctc
     1561 acccatgccc acgccccagt cggtttctac cacgaccacc tcaccgggcc ggagggggtc
     1621 attgggcgag acgcagtact ggtatcgggc cgaacccccg aattccacgc gtacgaggcg
     1681 agtcatgcct atcccctttg ccctctcccg attgcctggc gcatccggaa gagcatgcgt
     1741 tccgcggtca ggcgggggtt ggcatagcgg gccagggccc actgtgcttc cacctgcgcc
     1801 gcatagatgt tgaggatcgc gcccatcgag tgggccgccg ccagttgctc cgccgcggtt
     1861 cgcctgtccg ggggcaagag cgcgaccgcg ctccccccga ccttggccac cgccac
//
LOCUS       DBNGZC010001067         3173 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_483253, whole genome shotgun sequence.
ACCESSION   DBNGZC010001067 DBNGZC010000000
VERSION     DBNGZC010001067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_483253"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1520
                     /gene="trpE"
                     /locus_tag="AC16G0_18445"
     CDS             33..1520
                     /gene="trpE"
                     /locus_tag="AC16G0_18445"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIR5101641.1"
                     /translation="MYTPDLETFRRLATQGNLIPVARQILADMETPVSAFRKIDSGNY
                     AFLLESVEGGEQWGRYSFLGSNPSLVLRATGERLELLRGEGSEVLTDVRDPLTALKRV
                     LDEHKAVSVPGLPRFTGGMVGYLGYDIVRHIERLPSLSTDDLHLPDALFLLTDTLLIF
                     DNVTHQITVVSNASLRDHSPAGVEAAYRESQQKIEAIIAALRRPVGAPMGSAVRARAP
                     EPDSTFSREEFCKVVERAKAYVQAGDVIQAVLSQRLTVRTGADPFDVYRALRIVNPSP
                     YMYYLRLGELKIVGSSPEMLVRLEGERIDLRPIAGTRPRGKTEAEDLRLAEELLADPK
                     ERAEHIMLVDLGRNDVGKVARVGSVEVPELMTVERYSHVMHLVSHVRGALEPGRDAFD
                     VLRACFPAGTVTGAPKVRAMEIIEELEPKRRGPYAGAVGYASFSGNLDTCITIRTILF
                     SHGAATIQVGAGIVADSDPAGEYQETMNKARGLLRAIELVEDGLD"
     gene            1617..2207
                     /locus_tag="AC16G0_18450"
     CDS             1617..2207
                     /locus_tag="AC16G0_18450"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIR5101642.1"
                     /translation="MILMIDNYDSFTYNLVQYIRELGAEVEVHRNDRISLQEIRTMAP
                     ARIVLSPGPCTPAEAGICCDVIRGFGSTTPLLGVCLGHQCIGAAYGGRIIRAARLMHG
                     KTSPILHDGRTVFAGLPNPFEATRYHSLLIERQSIPGCLAISAETAEGEIMGVRHRSH
                     PVEGVQFHPESILTQSGKALLRNFLTQPAPVTEERR"
     gene            2204..3097
                     /gene="trpD"
                     /locus_tag="AC16G0_18455"
     CDS             2204..3097
                     /gene="trpD"
                     /locus_tag="AC16G0_18455"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR5101643.1"
                     /translation="MIIEAIRKVVDRQDLTRDEAFSVMDAIMSGQATDAQIAAFLTAL
                     RMKGETVEELIGFARVMREKVSLVKTRARVQTSLSDTGREMLVDTCGTGGDATGSFNV
                     STATAFVVAGAGIPVAKHGNRSVSSLCGSADVVEALGVRLDVPPDRVGKCIDEVGIGF
                     CYAPLLHKAMKYVMLARKEIRIRTVFNILGPLTNPAKASAQVIGVYDGKLTEVMAEVL
                     KELGTVRAFVVHGEDGLDEISTTCESRVAELRDGWMRSYTIRPEDFGLPRASMADLRG
                     RCAASTISIGDAEGPSASSPR"
     gene            3079..3173
                     /locus_tag="AC16G0_18460"
                     /pseudo
     CDS             3079..3173
                     /locus_tag="AC16G0_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018094983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
BASE COUNT          544 a         1037 c         1091 g          501 t
ORIGIN      
        1 gcggggacgc cccagttgcg cgaggcgata gcatgtacac tccggacctg gagaccttcc
       61 gccgattggc cacgcagggc aatctcattc cagtcgcgcg acagatcttg gcggacatgg
      121 agacccccgt ctccgccttc cgcaagatcg acagcggcaa ttacgccttc ctcctggaga
      181 gcgtggaggg aggcgaacag tggggacgct acagcttcct gggcagcaac cccagcctgg
      241 tcctgagggc cacaggggag cggctcgaac tcctgcgggg tgaggggagc gaggttctga
      301 cggatgtccg cgacccgctg acggccctga agcgggtgct ggacgagcac aaggcggtga
      361 gcgtgcctgg gcttccccgc ttcaccggcg gcatggtagg gtacctgggg tacgacatcg
      421 tccgccacat cgagcggcta cccagcctga gcaccgacga cctgcacctt cccgatgccc
      481 tgttcctgct caccgatacg ctgctcatct tcgacaacgt gacccatcag atcaccgtgg
      541 tgagcaacgc cagcctgcgc gatcactcgc ccgcaggggt ggaggcagcc taccgggaat
      601 cgcagcagaa gatcgaggcc atcatcgccg cccttcgccg gcccgtgggc gcgcccatgg
      661 gcagcgcggt ccgcgccagg gctccggagc cggattccac cttcagccgg gaggagttct
      721 gcaaggtggt ggagcgggca aaggcctacg tacaggccgg cgacgtgatc caggccgtgc
      781 tgtcccagcg gttgacggtc cgtacagggg ccgacccctt cgacgtctac cgcgccctcc
      841 gcatcgtgaa cccctccccc tacatgtatt acctccgcct gggggagttg aagatcgtgg
      901 gcagctcccc ggagatgctg gtcaggctgg agggggagcg catcgacctc cgccccatcg
      961 ccgggacgcg accgcggggc aagacggagg cggaggatct gcggctcgcc gaagagttgc
     1021 tggccgatcc caaggaacgg gccgagcaca tcatgctggt ggatctcggt cggaacgatg
     1081 tgggcaaggt ggcgcgggtg ggaagcgtgg aggtgccgga gctcatgacc gtggagcggt
     1141 actcccacgt catgcacctc gtgtcccacg tccgcggcgc gctggagccg ggacgggatg
     1201 ccttcgatgt gcttcgggct tgcttccccg cggggaccgt cacgggtgcc cccaaggtcc
     1261 gggccatgga gatcatcgag gaattggagc ccaagcgccg cggcccttac gccggggcag
     1321 tcggctacgc cagcttctcc ggcaacctcg acacgtgcat caccatccgc accatcctgt
     1381 tctcccatgg agcggccacc atccaggtgg gggccggcat cgtggcggac tccgacccgg
     1441 cgggagagta ccaagagacc atgaacaaag cgcggggatt gctgcgggcg atcgagctgg
     1501 tagaggacgg actggattag cgcatggagc gttcggcggt tcgggtgttc ggccgtccag
     1561 gcaaaagttg attcgacgaa ctgccgaaca gccgggctca cgaacggggc ttcgccatga
     1621 tcctcatgat cgacaactac gactcgttta cctacaacct cgtccagtac atccgggaac
     1681 tcggcgccga ggtcgaggtg caccggaacg accggatctc gttgcaggag atccggacca
     1741 tggctcccgc gcggatcgtc ctctctccgg gcccctgcac cccggccgag gcgggaatct
     1801 gctgcgatgt gattcggggc tttggctcca cgacgccgct cttgggcgtg tgtctcgggc
     1861 accagtgcat cggcgccgct tacggaggca ggatcatccg ggccgcgcgg ctcatgcacg
     1921 ggaagacctc gcccatcctc cacgacggac ggaccgtgtt cgccggactc cccaatccct
     1981 tcgaggccac acgctaccac tcgctcctca tcgagcggca gagcatcccc gggtgcctcg
     2041 cgatctcggc ggagaccgcc gagggggaga tcatgggtgt ccggcatcgg tcgcaccccg
     2101 tggagggcgt gcagttccac ccagagtcca tcctgaccca gtcggggaag gcgttgctga
     2161 ggaatttcct gacccagccc gcgcccgtca cggaggagcg ccgatgatca tcgaagcaat
     2221 tcgcaaggtc gtggaccgcc aggacctcac ccgcgatgag gccttcagcg tcatggacgc
     2281 catcatgtcc ggtcaggcca cggacgccca gatcgccgcc tttctgaccg cgctccggat
     2341 gaagggggag acggtggagg agctgatcgg cttcgcccgg gtgatgcggg agaaggtctc
     2401 gttggtaaag acgcgggccc gggtccagac ctcgctctcg gacacgggcc gggaaatgct
     2461 ggtggatacc tgcggcaccg gcggcgacgc cacggggagc ttcaacgtct ccacggccac
     2521 ggcctttgtc gtggctgggg ccggcatccc ggtcgccaag cacggcaatc gctccgtcag
     2581 cagcctgtgc ggcagcgccg acgtagtgga ggcgctcggc gtccgccttg atgtcccccc
     2641 ggatcgggtg ggcaagtgca tcgacgaggt cggcatcggc ttctgctacg ccccgctcct
     2701 gcacaaggcc atgaagtacg tgatgctggc ccggaaggag atccggatcc gcacggtgtt
     2761 caacatcctt ggcccgctga ccaaccccgc caaggcctcg gcgcaggtga tcggggtgta
     2821 cgacgggaag ctgaccgagg tcatggccga ggtgctgaag gaactgggga cggtgcgggc
     2881 cttcgtggtc cacggggagg acggtctgga cgagatctcc accacctgcg agagtcgggt
     2941 tgcggagctg cgggacgggt ggatgcgaag ctacacgatc cgcccggagg acttcggcct
     3001 gccccgagcc agcatggcgg atcttcgggg gcggtgcgcc gcgagcacga tcagcatcgg
     3061 agacgccgag ggcccgtccg cgtcatcgcc gaggtgaaga aggcatcccc gtccaaaggg
     3121 gtgatccgac ccgacttcgt ccccgcggaa ctggcgcgcg cctatgccgg ggg
//
LOCUS       DBNGZC010001068         3573 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_776891, whole genome shotgun sequence.
ACCESSION   DBNGZC010001068 DBNGZC010000000
VERSION     DBNGZC010001068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_776891"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..808
                     /locus_tag="AC16G0_18465"
     CDS             <1..808
                     /locus_tag="AC16G0_18465"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004793 - threonine aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HIR5101644.1"
                     /translation="LERDANIYTSEVGGVAGVGGLVVNRVPGTDGVLAPSDVEAAIRP
                     ARLHSPVTGLICVENSHNTAGGTVWTPAQMAAVKAVAVRHGIPVHLDGARIFNAAVAL
                     GVQAREIARHTDSATFCLSKGLSAPVGSVIVGSKAFIQTARKMRQMLGGTTRQGGVIA
                     AAGLVALRTMIDRLAEDHAHARILAEKLSTIPGLEVHVKKVQTNIVRTKTSGLGVPAA
                     TVAEKLAQHNLYVLAQQADTLRWLTHRHVTAGDVDEAAATMTKVAEVLRR"
     gene            853..1806
                     /locus_tag="AC16G0_18470"
     CDS             853..1806
                     /locus_tag="AC16G0_18470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR5101645.1"
                     /translation="MRFGFPIPTRGALATLDAIRRLGQAADRYQYDSVWITDHVVIPK
                     ATTSKYPYSPDGSFDLGAAQHYLDALTVMAYLAAVTQRTAIGSSVLILPYRNPMLVAK
                     MVATLDVLSQGRAILAVGVGWMREEFAALNLTTFEERGAATDEYIRILRELWTKEWPS
                     FQGRFFSFDEVRFYPKPVQKPHPPIWIGGHTKAAIRRAALLGDGWHPIGLRPPAGLYP
                     EDYAKAAADLRAQAEAAGRDPKAISLSLRAPIRFTDGGTSGSRTPFIGSRDQIVEDIR
                     TYQRLGVSHLIFDVAGTSIEAILEQLQRFSEEVRPVVRGRP"
     gene            1936..2388
                     /locus_tag="AC16G0_18475"
     CDS             1936..2388
                     /locus_tag="AC16G0_18475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HIR5101646.1"
                     /translation="MLTIAPEDRAFLHQARVGRLATASAAGEPHVVPVCFVFDGTCLY
                     SAIDAKPKRVEPRRLRRLLNLRENPRACLIVDHYEEEWARLRYVLVSGRVEVLETGSD
                     RERALVLLQEKYRQYRSMPGFGQGPVIRLIPEKVATWRGASTRSKPPL"
     gene            2630..3094
                     /locus_tag="AC16G0_18480"
     CDS             2630..3094
                     /locus_tag="AC16G0_18480"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HIR5101647.1"
                     /translation="MQIGPNTTVTIDYTLRLDSGEVVDSSSESGPLTFTFWQGQIIPG
                     LERELVGLQNGDQKEIRVTSEEGYGERQTEALQQVPVDRFPTDITPEPGFRLTVRGPQ
                     GEEVPFTIVAVADGKVTRDFNHPLAGEALNFSVTVRDVQAEGGKRLIVPGEA"
     gene            3094..3402
                     /locus_tag="AC16G0_18485"
     CDS             3094..3402
                     /locus_tag="AC16G0_18485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101648.1"
                     /translation="MAMRTEVEDVVETSSDTETGGFPGLCRVCGGVVPRAGDQTSCTP
                     SICSEQCLARAIVEYWAAFGVEAQVSLRDGFARLEGLRGILPRTHSTAPQSIPQVSRE
                     "
BASE COUNT          633 a         1127 c         1184 g          629 t
ORIGIN      
        1 cctggagcga gacgccaaca tctacacgtc cgaggtgggc ggagtcgccg gggtcggcgg
       61 cctcgtcgtg aaccgggtcc ccggcacgga cggcgtcctg gcgccgtcgg atgtcgaggc
      121 ggcgatccgg ccggcgcgcc tccactcccc ggtgacgggc ttgatctgcg tggagaacag
      181 ccacaacacg gctggaggca cggtctggac accggcgcaa atggcggcag tcaaggcggt
      241 cgcggtgcgg cacgggatcc ccgtccacct cgacggggcc cggatcttca acgcggccgt
      301 ggccctgggg gtccaggctc gtgagatcgc ccggcacacc gactcggcca cgttctgtct
      361 gtccaagggc ctgagcgcgc ccgtgggctc cgtgatcgtg ggatcgaagg ccttcatcca
      421 gacggctcgc aagatgcggc agatgctggg tggaacgacg cgtcagggcg gcgtgatcgc
      481 ggccgcggga ctggtggccc tccggaccat gattgatcgc ctggcggagg atcatgccca
      541 cgcacggatt ttggcggaaa agctcagcac gatccccgga ctggaggtgc acgtgaagaa
      601 ggtccagacg aacatcgtgc ggacgaagac ctcgggtctg ggcgtccccg ccgccaccgt
      661 ggccgagaaa ctggctcagc acaatctcta cgtactggcg cagcaggcgg acacactgcg
      721 ctggctgacc caccgacacg tgacggcggg cgacgtggat gaggccgccg ccacgatgac
      781 caaggtagcg gaggtgctgc gaaggtaggg agcggcccgg aaatggtgaa cgggatccga
      841 aaggagtcgg ccatgcgctt cggatttccg atccctacac gcggcgccct ggcgacgctg
      901 gacgccatcc ggaggttagg tcaggccgcg gataggtacc agtacgattc ggtctggatc
      961 accgaccacg tggtcatccc gaaggccacg acctccaagt atccctactc gcccgacggc
     1021 agcttcgatc tcggcgcggc ccagcattat ctcgacgcgc tcacggtcat ggcctacctg
     1081 gcggcagtga cgcagcgcac ggccatcggg tcgagcgtcc tgatcctgcc gtaccggaac
     1141 cccatgctcg tggccaagat ggtggccacg ctggacgttc tatctcaggg gcgcgcgatc
     1201 ctggcggtcg gggtgggctg gatgcgcgag gagtttgccg ccctgaatct cacgaccttt
     1261 gaggagcgcg gggccgccac ggacgagtac atccggatcc tgcgggagct gtggacgaag
     1321 gagtggccga gcttccaggg tcggttcttc tcattcgacg aggtccggtt ctatccgaag
     1381 ccggtgcaga agccgcaccc gcccatctgg atcggcgggc acaccaaagc agccatccgg
     1441 cgggcggcac ttctcggcga cggatggcat cccatcgggc tgcgcccgcc cgccggactt
     1501 tacccggagg attatgcgaa ggcagccgcc gacctccgcg cccaggccga ggcggcgggc
     1561 cgggacccca aggcgatcag cttgtccctg cgcgcgccga tccggtttac ggatggtggg
     1621 acgtcgggca gccgcacgcc cttcatcggc tcgcgggatc agatcgtgga agatattcgc
     1681 acctatcagc gcctcggggt gagccacttg atattcgatg ttgccggaac gagcatcgag
     1741 gccatcctgg agcaactcca gcgcttctcc gaagaggtgc ggcccgtggt caggggccgg
     1801 ccttaggtaa gagcgggatt ctcaaatgta aaaccgacaa cggacaaccg gtcagctcaa
     1861 acattggccg aaattgatcg gttgtccgtt gtcggttgtc cgttttgcat tggctcttgc
     1921 tgccgctccg ccagcatgct caccatcgct cctgaggacc gtgcattcct gcaccaggct
     1981 cgggtcggca ggctcgccac ggcctcggcg gccggcgagc ctcacgtcgt cccggtctgc
     2041 tttgtcttcg acgggacctg cctgtactcc gccatcgatg ccaagcccaa gcgcgtggag
     2101 ccgcggcggc tccgccggct cctgaacctc cgggagaacc cgcgggcctg tctgatcgtg
     2161 gatcactacg aagaggaatg ggcgcgtctc cgctacgtcc tggtgagtgg cagggtcgag
     2221 gtgctggaaa ctggttcgga ccgggagcgg gccttggtgt tgctccagga aaagtatcgc
     2281 cagtatcggt ccatgcccgg cttcggtcag ggtcctgtca ttcgtctcat tcctgagaaa
     2341 gttgccacct ggcgcggtgc ctccacgcgc tccaagcctc ccctctgact tgaaagtctt
     2401 tcttggcctc ggagtccttg gcgctatgct ggttaggggc tctgaatcca gtcgaatcca
     2461 gtcgaaatgc cttgacagaa caggctattt cgcctacttt tccacgacaa gaccggctgg
     2521 cgcatagggc ccacagatag tttttgcgcg gcaattgccc ccaaagactc tcttaggtgt
     2581 tcttgggggt tttgatttct ccggacactg ggagtcaagg agaggtcagg tgcagatcgg
     2641 accaaacacc accgtaacaa ttgactacac gctgcggctg gactcagggg aagtcgtgga
     2701 ctcttcaagt gagagcgggc ccctgacctt cacgttctgg cagggccaaa tcattcccgg
     2761 cctggagcgc gagctggtgg gtttgcagaa cggcgatcag aaagagattc gcgtgacttc
     2821 cgaagaggga tacggggagc gacaaaccga ggccctccaa caagtaccgg tggatcgctt
     2881 tcccacagac atcacgcccg agcccggatt ccggctcact gttcggggcc ctcaaggcga
     2941 agaggtgccc ttcaccatcg tcgccgtcgc cgacggaaaa gtcacacggg atttcaatca
     3001 ccccttggcc ggcgaggccc tcaacttctc cgtgaccgtg cgcgacgttc aggcggaggg
     3061 gggaaagcgg ctgatcgtcc cgggggaggc atgatggcca tgcggaccga agtggaggat
     3121 gtggtcgaga cttcatcgga taccgagacc gggggattcc cgggcctctg ccgagtctgc
     3181 ggcggcgtcg tgccgcgggc gggcgaccag acgagttgca cacccagcat ctgcagcgag
     3241 cagtgtctgg cccgggccat cgtggagtac tgggcggctt tcggcgtgga ggcgcaggtg
     3301 agcctgcgtg atggcttcgc ccgtctggaa ggccttcgcg ggatcctgcc ccgcacgcac
     3361 tccaccgccc cgcagtcgat tcctcaggtt tctcgggagt agccggcagg gggcgggtat
     3421 agcttcggtc cggaggccgt cccgccccat catcgcgttc atacgcccac aagccgcaca
     3481 tacggctcgc caatgaaagt ccggccgtgg cgcgccgaca agctagctcc ttgcacagcc
     3541 tgagttcaaa atctcactgc gaagcctgca aag
//
LOCUS       DBNGZC010001069         3181 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_679386, whole genome shotgun sequence.
ACCESSION   DBNGZC010001069 DBNGZC010000000
VERSION     DBNGZC010001069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_679386"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            449..1522
                     /locus_tag="AC16G0_18490"
     CDS             449..1522
                     /locus_tag="AC16G0_18490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014653.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HIR5101649.1"
                     /translation="MTDYASKRVNPRRLERLVSSAFQRVGVPLSEADLTATILVDADL
                     HGIDSHGVLNLYGTYIKGLQTGSINPTPEIKISQGSPTTVSVDGDRGLGLLVSHRAMG
                     ECIRMAQEYGTGWATACNSTHSGAGAYYVRMAAARRMIGIHCSTGGSTVAAPGGKSRL
                     IGNNVIAFGAPGGHHGPFVLDMAPTMAIANKLHMLQWEGKRMPEGWAIDAAGRPITDP
                     NVYFATEGAILPLGSTPAYGVHKGFGLLLLSDVLTGLLSGDGGSLLRRKGEHSHAFCA
                     LRVDAFPTGGEFTALMDVMVEKLHATPTVEGAGRIRYPGERSNLTYQERSAKGIPLRQ
                     YVVDDLRTMSLELHLSMDDIWEE"
     gene            1715..2926
                     /locus_tag="AC16G0_18495"
     CDS             1715..2926
                     /locus_tag="AC16G0_18495"
                     /inference="COORDINATES: protein motif:HMM:NF012885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101650.1"
                     /translation="MTDESTSMPWRTDHWFCSPHNFDPDIRVPPHPAELHDITVRDGE
                     ECADVAFSVEDKVRIAEALALVGVKRMELFLTVPGWLEAVRAILRRKLPLALYVTWHP
                     GRVERVLDLGVRHIMVWYRILEEHQRHDLRRSRAELLDQAANQIAAARQGGCEANLFI
                     VEATRAPLDQLKETVRVGKQAGASAVTVVDSFGVARPAAIAFLVGQVREWSGLAVDVH
                     CHNDFGLSTANVLAAYEAGATGLNVAVNGLGYRAGNAALDEVAMALKVLYGLDTGLRL
                     ELLPWLSHLVEEITGIPVDYFKPIVGKGAFSVEQWKTGNAMETAGARPVVFPFEPEVI
                     GRSPRVVVGKWSDVGAVAKKLTEYGLTASPEQLNQILVACQRAGIAHHRPLGDDEFLA
                     IATAGGARASE"
BASE COUNT          564 a          969 c         1080 g          568 t
ORIGIN      
        1 tggtcggcat ggcaacctcc tatgtgaatt cgcggaccac tccgaagcaa tgcccgtgcc
       61 aggccatcgg actctggcat ccggcccgcc tattggcgac cctgggccca ataggcgggc
      121 cggatcccac agattcccgg tggtttctga aatgccgcag attgtacgcg gtttctgccc
      181 ccgatcaggg cggcaatgct tgctgtcagt ggggccagtc taccgtccga aaactcacac
      241 aggctgtctg agagtgtgtc acagcggaac tcttattgac gaagtggaag aaccttagta
      301 gggtcgattc gcgaatcggg aagaagggac cgggacaggg ggccggcatg gtctccggat
      361 tgcgcgaatt ggtgtggtga ccgcggcggc tccggccgcc ggcttctccc gatcccacgt
      421 gttcctcggg tcacaagggg agagagcgat gacggattat gcatcgaagc gggtgaatcc
      481 acggaggctc gagcggctcg tctcatctgc atttcagcgg gtgggtgttc ccctcagcga
      541 ggcggatctg accgccacga tcctcgtgga tgctgatctg cacggcattg actcgcacgg
      601 ggttctcaac ctctacggga cctacatcaa agggcttcag acggggagca tcaatcccac
      661 ccccgaaatc aagatttccc aaggcagtcc cacgacggtg tccgtggacg gcgacagggg
      721 gctgggactg ctggtcagcc accgggccat gggcgaatgc atccggatgg cgcaggagta
      781 cgggacgggg tgggccacgg cgtgcaacag cacgcactcc ggcgctggcg cctactatgt
      841 ccgcatggcg gccgcccgga ggatgattgg cattcattgt tccacgggcg gatcgacggt
      901 ggctgcccca ggagggaaga gccggctcat cggcaacaac gtcatcgcct ttggggcccc
      961 cggggggcac cacgggccct tcgtcctgga catggcgccc accatggcca tcgccaacaa
     1021 gctgcacatg ctccagtggg aaggtaagcg gatgcctgaa gggtgggcca tcgacgccgc
     1081 cgggcgaccg atcacggatc cgaacgtcta ctttgcaacc gaaggcgcca ttctccccct
     1141 gggcagcact cccgcgtacg gtgtccacaa gggatttggc ctcctgctgc tgtctgatgt
     1201 tctcaccggg ctcttgtctg gggacggcgg cagcctgctc cgccgaaagg gggagcacag
     1261 ccacgccttc tgcgcgcttc gtgtcgacgc cttcccgacg ggcggcgagt tcacggcgct
     1321 catggacgtt atggtcgaga aactccacgc caccccgacc gtcgagggcg ctggacgtat
     1381 ccggtatcct ggcgaacgta gcaatctgac ctatcaggag cgaagcgcca aagggatccc
     1441 gcttcgccag tatgtggtgg atgatctgcg aacaatgagc ctggagctgc acctttcgat
     1501 ggacgacatc tgggaagagt gacctctcag acccattcgg ttgttctgtt tatcggtcat
     1561 atacgttcca ggctcctctt cttgccttcg agtgtgttag cctcccgccg ttactgcaca
     1621 ggtctggggg tatcgtcgct ttcgctaact gtgcccaccc aaacaagccc tggaatctag
     1681 gcaaaccgct ccatatcgaa agcaggtgat tgcgatgacg gacgaatcga cctcgatgcc
     1741 ctggcggacg gatcactggt tctgcagccc gcacaacttc gatcctgaca tccgggtgcc
     1801 gccccatccg gcggaactcc acgacatcac ggtgcgggac ggtgaagagt gcgcggacgt
     1861 cgcattcagc gtggaagaca aggtgcggat cgccgaggcg ctggcgctgg tgggcgtcaa
     1921 gcgcatggag ctgttcttga cggtccccgg atggctggag gcggtgcggg cgatcctccg
     1981 gcgcaagctg ccgctggcgc tttacgtgac gtggcacccc gggcgggtcg agcgcgtcct
     2041 ggatctgggt gtgcggcaca tcatggtgtg gtaccggatc ctggaggaac accagcgaca
     2101 cgacctgcgg cgatcccggg cagagctgct ggaccaagcg gcgaaccaga tcgcggccgc
     2161 ccggcagggg ggttgcgagg caaacctgtt catcgtcgag gccacccgcg caccccttga
     2221 tcagttgaag gagaccgtgc gcgtaggcaa gcaggcaggg gcctcggcgg ttaccgtggt
     2281 ggatagcttc ggggtggccc ggccggccgc catcgccttt ctggtcgggc aggtgcggga
     2341 atggtccggc ctggccgtgg acgtccactg ccacaacgac ttcgggctgt ccacggcgaa
     2401 cgtcttggcg gcctacgaag ccggagcgac cgggttgaac gtggctgtca acgggctcgg
     2461 ctatcgggca gggaacgcag cgctggacga ggtcgccatg gcgctgaagg tcctgtacgg
     2521 cctggatacc gggttgcgtc tggaactgct gccctggctg agtcacttgg tcgaggagat
     2581 cactggcatc cccgtggact acttcaagcc tatcgtgggg aagggggcgt tcagcgtaga
     2641 gcagtggaag acgggcaacg cgatggagac ggcaggcgcc cgccccgtgg tattcccctt
     2701 cgagcccgag gtcatcggcc gctcgccgcg cgtcgtggtg gggaaatgga gcgacgtggg
     2761 tgccgtggcc aagaagctga cggagtatgg cctcaccgcc tcgcccgaac aactgaatca
     2821 gatcctggtc gcctgccagc gggccgggat cgcccatcac cgcccgcttg gcgatgacga
     2881 gttcctggcg atcgcgacgg cgggaggcgc ccgggcgagc gagtgaggcc tgcttctgcg
     2941 cggtgtcgtg ggcggccatg acggtcgtcc gaggcgactg agggacctgg cgcagggaag
     3001 gcatgcacga ggagggaaag atgaaaaagc gtctgacagc acgcccaagt cgatcaggct
     3061 tcccagttct taccggcggg ttgctcattg ctgcgctggc cgtgctctgg acggcggtcg
     3121 cacccgaggc gcgtgcggcg gtcgatatgc gcctgaccca tcccgccgac ctgacgcacc
     3181 c
//
LOCUS       DBNGZC010001070         1552 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2244563, whole genome shotgun sequence.
ACCESSION   DBNGZC010001070 DBNGZC010000000
VERSION     DBNGZC010001070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2244563"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1328
                     /gene="thrC"
                     /locus_tag="AC16G0_18500"
     CDS             33..1328
                     /gene="thrC"
                     /locus_tag="AC16G0_18500"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007222783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR5101651.1"
                     /translation="MGYVKGLKCRECARPYPAKPLHVCEFCFGPLEVDYDYPAIGAAM
                     TRQGIEAGPPSIWRYGPLLPVDGEVTVGRYCGFTPLVQARNLGKALGLNRLYVKNDTV
                     NHPTLSFKDRVVAVAVNKAREFGFPVVACASTGNLANSVAAHAAEGGLSSFVFIPSDL
                     EQGKVLGTSIYAPNVVAVDGTYDEVNRLCSEVADKYGWAFVNINIRPYYGEGSKTFGY
                     EIAEQLGWRAPDHVVVPAAGGSLITKIWKGLHEFHKLGFIPGVPTRMHVAQAAGCGPI
                     VTAVKAGSEIIRPVKQPRTIAKSLAIGNPADGYYAFKVVKDSGGAGEHATDEEIVEAM
                     KLLAATEGIFAETAGGVTVAATKKLVQQGAIGRDEVTVISVTGNGLKTQEALTAALRV
                     PLHIKPSLKSFDEALAQASAIGAAGRDPSGDELLSQKGV"
     gene            1334..>1552
                     /locus_tag="AC16G0_18505"
     CDS             1334..>1552
                     /locus_tag="AC16G0_18505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018285917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR5101652.1"
                     /translation="MSVLVRIPTPLRAITKGQGELKAEAVSVKELIDRLDQEYTGLRT
                     RLCEDDGNIRRFINVYVNEEDIRFLQGTA"
BASE COUNT          303 a          480 c          526 g          243 t
ORIGIN      
        1 ggcatgccag aagctcagga ggcaatgctg atatggggta tgtgaagggt ctgaagtgcc
       61 gcgagtgcgc gcgaccgtac ccggcgaaac cattgcatgt ctgcgagttc tgtttcggcc
      121 cgctggaagt ggactacgac tacccggcca tcggggcggc catgacccgg caggggatcg
      181 aggccgggcc gccgagcatc tggcgctatg gccccctcct gcccgtggac ggcgaggtga
      241 ccgtcggccg gtattgtgga ttcacgccgc tggtccaggc cagaaatctg gggaaggccc
      301 tgggcctcaa ccgcctctac gtcaagaacg acacggtcaa ccatccgaca ctctccttca
      361 aagaccgggt ggtggccgtg gccgtcaaca aagcacggga gttcggcttt ccggtggtgg
      421 cttgcgcctc cacaggcaac ctggcgaact cggtggcggc ccacgcggcc gaggggggat
      481 tgagcagctt cgtgttcatc ccgtccgacc tggagcaggg gaaagtcctg gggacctcga
      541 tctatgcccc caacgtcgtg gcggtggacg gcacctacga cgaggtgaat cggctgtgca
      601 gcgaggtggc ggacaagtac ggctgggcct tcgtgaacat caacatccgc ccgtactacg
      661 gcgagggttc caagaccttc ggttacgaga tcgccgagca gttgggctgg cgggcgcccg
      721 accacgtcgt cgtcccggcg gcagggggct cgctcatcac gaagatctgg aagggtctgc
      781 acgagttcca caagctcggt ttcatcccag gcgttcccac gcggatgcac gtcgcccagg
      841 cggccggctg cggtccgatc gtgactgcgg tgaaggcagg gagtgaaatc atccgcccgg
      901 tgaagcaacc ccgaaccatc gccaagtcgc tggccatcgg caaccccgcc gacggctatt
      961 acgccttcaa ggtggtcaag gacagcggcg gcgcggggga gcacgccacc gacgaggaga
     1021 tcgtggaggc catgaagctc ctggcggcca cggaagggat cttcgccgag acggcagggg
     1081 gcgtcactgt cgccgcgacc aagaagctgg tgcaacaggg ggccatcgga cgcgacgagg
     1141 tcacggtcat ttccgtgacc ggcaacgggt tgaagaccca ggaggcccta acggccgccc
     1201 tccgagtccc cctccacatc aagccgagcc tcaagtcctt cgacgaggcc ctggcccagg
     1261 cgtcggcgat cggcgccgcg ggccgggatc cgtcggggga cgagcttctc tcacaaaagg
     1321 gggtgtgact accatgagtg tgctggtgcg aattcccacg ccgcttcgcg cgatcaccaa
     1381 agggcagggc gaactgaaag ccgaagcggt cagcgtcaag gaattgatcg accggttgga
     1441 ccaggaatac actggcctca gaacacgact ctgcgaggac gacgggaata tccgccgctt
     1501 catcaacgtc tacgtgaacg aagaggacat ccgcttcctg caggggacgg ca
//
LOCUS       DBNGZC010001071         1456 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_288100, whole genome shotgun sequence.
ACCESSION   DBNGZC010001071 DBNGZC010000000
VERSION     DBNGZC010001071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1456)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_288100"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="AC16G0_18510"
     CDS             <1..692
                     /locus_tag="AC16G0_18510"
                     /inference="COORDINATES: protein motif:HMM:NF018894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR5101653.1"
                     /translation="EPGDTVGDQTVRHLARGADVLIYDAQFAPALLERRKGWGHSTWV
                     EAIKVAKDADVRCLMLFHHDPDSNDACVDRFVQLARDQWPRTWGAAEGYQITCRTPEV
                     RVETAAPRVGPRVTARLPVRLRGKRRDGSAIDHEAVLSNLSLKGTFVVVPEPPDLNSE
                     VEIVLMESGESEAVITGQVVRIDLDPETGQPAVGIVFPMETRRIRPLAGPGGPEASER
                     SALTTPGEIPP"
     gene            894..>1456
                     /locus_tag="AC16G0_18515"
     CDS             894..>1456
                     /locus_tag="AC16G0_18515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIR5101654.1"
                     /translation="MAPIPVLTEASILPSRYQVPEACTLVIFGASGDLTKRKLLPALY
                     ALAHDGLLPERFAVVGFARREKSHEAFREEMRAAVEEFSRLRPPKPEIWDHLAQGLYY
                     LSAAFEDAAGYERLRDLLAETDRRHETGGNRVYYLATPPSAYSAIVARLGAAGLVSNA
                     WDGPQDDAGGAPGVGWSRIIIEKPFGR"
BASE COUNT          250 a          465 c          495 g          246 t
ORIGIN      
        1 ccgagccggg ggataccgtg ggagaccaga ccgtccggca tctggcccgg ggggccgatg
       61 tcctgatcta tgacgcccag ttcgcaccgg ccctgctcga acggcggaag ggttggggtc
      121 acagtacctg ggtcgaagcg atcaaggtag caaaagacgc ggatgtccgc tgcctgatgc
      181 tgttccacca cgaccccgac tccaacgacg cctgcgtgga ccgattcgtc cagctggccc
      241 gcgatcagtg gcctcgaacg tggggagctg ccgaggggta ccaaatcacg tgtcggacac
      301 ccgaggtccg ggtggagacc gccgcccctc gcgtcgggcc acgggttacc gcgcggcttc
      361 cggtgcgcct gcgcgggaaa cgaagagacg gctcagccat cgatcacgaa gccgtgttgt
      421 ccaacctgtc cctgaaggga accttcgtgg tggtgcccga gcctcccgac ctgaattcgg
      481 aggtcgagat cgtgctgatg gagtccgggg agagcgaggc ggtgataacc ggccaggtcg
      541 tccggatcga tttggacccg gaaaccggtc aacctgcagt cggcatcgtc ttccccatgg
      601 agacacggcg aattcgaccg ttggccggcc cggggggacc ggaggcgagt gagaggagcg
      661 cgctcacgac gcccggggag atcccgccct gatcggtggt tgccgtccct tccctcgccg
      721 ctccctcgtg gttgatcaaa tcccagacgc atggccggat cctgaggtcc cgggccaggt
      781 gatgggtggt atccggccgt gtccgtcagg cctcttccag gcaaggaccg agaaatcctc
      841 cgtccttggc cctcctggcg ccttggcggt tgatgtcctg aggtgatatg cccatggcac
      901 ccattcccgt tttgaccgag gcctcgattc tgccctcgcg ctaccaggtc ccggaggcct
      961 gcaccctggt gatcttcggg gcatccggag acctgacgaa gcgcaagctc ctgccggccc
     1021 tctacgccct ggcacacgac ggcctgctgc cggaacggtt cgcggtggtc gggttcgcgc
     1081 ggcgggaaaa gagtcatgaa gccttccgag aagagatgcg agcggcggtg gaggagtttt
     1141 cccggctccg tccaccaaag ccggagatct gggatcatct ggcccaggga ctctactacc
     1201 tctcggctgc gttcgaagat gcggccggtt acgagagatt gagggacctc ctagccgaga
     1261 cggatcgccg ccacgagaca ggcggcaacc gggtgtacta cctggccacc cctcccagcg
     1321 cctactccgc aattgtcgca aggctggggg cagcgggtct ggtgagcaat gcctgggacg
     1381 gcccccagga tgacgcgggg ggagcacccg gcgtgggctg gagccggatc atcatcgaga
     1441 aacccttcgg tcggga
//
LOCUS       DBNGZC010001072         2542 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2638269, whole genome shotgun sequence.
ACCESSION   DBNGZC010001072 DBNGZC010000000
VERSION     DBNGZC010001072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2638269"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="AC16G0_18520"
     CDS             complement(<1..1273)
                     /locus_tag="AC16G0_18520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HIR5101655.1"
                     /translation="MTMAQDDILLEVRAEDLKQEQKKLWAVAFGVIAFLFTAFQLYTA
                     GAGPYIALVQRAVHVCFAVVLCAMAYRMKMEKAGRQPLAAYLFDLVEIVATLAVTVYL
                     VMNVERLVMQIGFARPNMTDTIFGYVMTFVVINASRKAIGWAFTWMALAFIAYSILGP
                     WAPGLLAHRGYTPNEVAALLLVNPLGIYGAITGTSANIIAIYIIFGAVMLRTGAAKAF
                     LNLALMLAGRLTGGAGQVAVVASAMMGMVNGSAVANAATVGAFTIPMMVKRGYPRPFA
                     GGVESAASSGGQITPPVMGVGAFIMAEILGIPYLKIAQMAAIPAVLYYVGLGMVVYFE
                     AKRRNLGRLSGSDIPRWRTYWKELLLLLVPVIVLVYLIVMMFSPRYAGFWAVAINIVM
                     FFGFMIPFGKGFSLERAAGSIKNSGSQIVAGM"
     gene            complement(1408..2409)
                     /locus_tag="AC16G0_18525"
     CDS             complement(1408..2409)
                     /locus_tag="AC16G0_18525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HIR5101656.1"
                     /translation="MKTGKWRLLVLVALVGLAVVLSSPSAQAGTAGLKADWQTREYFF
                     GGWPSTAGQYMWAALTWTIIANDTGLRITVVDTAGTEESMHMLQNKKADMANYDAALV
                     KRDFKDKHDLRTIFPFAPAVWQFAVAKDANIKTLKDLDGKKWNPGPAGGGSTFVTMQI
                     MDMLGIKPVYHQATLTDAGEAYSDRQIVGFTYRGTGGDPTSAMVEANAARPVYFVSLT
                     EEEIAKIHAKWPNLNKYVVRAKLYPGQNDPFTTIATLTGNAIAASKDMPEEAVYTVVK
                     SYWKNFKNIAKQFPGVSGASPNDVVASEIPLHMGAIKYYEETGIKIPQSLYPPEAKK"
BASE COUNT          462 a          824 c          772 g          484 t
ORIGIN      
        1 ccatgccggc cacgatctgc gagccggaat tcttgatgct ccccgcagcc ctctcgaggc
       61 tgaagccttt tccaaacggg atcatgaagc cgaaaaacat gacgatgttt atggccactg
      121 cccagaatcc cgcgtaccgg gggctgaaca tcatgacgat caggtacacc aggacgatca
      181 cgggcaccag gagcaggagc agttctttcc agtaggtccg ccacctgggg atatccgagc
      241 cggacaagcg gccaaggttc ctcctctttg cctcgaagta gaccaccata cccaatccga
      301 cgtaatagag cacggccggg atcgccgcca tctgggcgat ctttaggtag gggatgccga
      361 ggatctccgc catgatgaag gcacccaccc ccatgaccgg gggagtgatc tggccgccgc
      421 tggaggccgc cgactccacg cccccggcga agggacgcgg atagccccgt ttcaccatca
      481 tcggaatggt gaacgcaccc acggttgcgg cattggccac ggcgctgccg ttcaccatgc
      541 ccatcatggc gctggcgacc acggcaacct ggccggcgcc acccgtcagc cgaccggcca
      601 gcatcagggc caggttcagg aaagccttgg ccgccccggt ccgaagcatg acggcgccga
      661 agatgatgta aatggcgatg atgtttgccg aggtcccggt gatcgctccg tagatcccca
      721 gtggattcac cagcaggagc gccgccacct cgttgggggt gtagccacgg tgggccagca
      781 ggccgggggc ccacgggccc aggatgctgt aggcgatgaa ggcgagggcc atccaggtga
      841 aggcccagcc gatggctttt cgagaggcat tgataaccac gaacgtcatg acgtacccga
      901 aaatcgtgtc cgtcatgttc ggcctggcga agccgatctg catcaccagc cgctcgacgt
      961 tcatgaccag gtacaccgtg acggcaaggg tggccacgat ctccacgagg tcgaagaggt
     1021 aggcggccag gggctgtcgc cccgccttct ccatcttcat ccgataggcc atggcgcaca
     1081 ggacgacggc gaagcagacg tggaccgccc gctgaaccag ggcaatgtac gggccggcgc
     1141 cggcggtgta gagctgaaac gccgtgaaca ggaaggcgat gactccaaag gctaccgccc
     1201 agagcttctt ctgctcctgc ttgagatctt cagcccggac ctcgagcagg atgtcatctt
     1261 gcgccatcgt catgggcgtg aaccctccag tctggaccta atggacgttc ttgctggaac
     1321 acccccctcg cccctcccgt cgggcatgcc ccgaggcgag cgcaggggcg cacccggggc
     1381 acgcttgacg gcccttccgc tgccaggcta tttcttggct tcaggcgggt agagactctg
     1441 cgggatcttt atcccggttt cctcgtagta cttgatcgcc cccatgtgca ggggaatctc
     1501 gctcgcgacc acatcgtttg gcgacgctcc cgagacgccg gggaattgct tggcgatatt
     1561 cttgaagttc ttccagtagg acttcaccac cgtgtagacc gcctcctcgg gcatgtcctt
     1621 gctggcggcg atcgcgttgc ccgtcaacgt ggcgatggtc gtgaacgggt cgttctggcc
     1681 ggggtagagc ttcgcccgca ccacgtactt gttcaggttg ggccactttg cgtggatctt
     1741 ggcgatctcc tcctcggtca ggctgacgaa gtagacgggc cgcgccgcat tggcttccac
     1801 catggcgctg gtgggatcgc cgccggttcc gcgataggtg aaccccacga tctggcggtc
     1861 cgagtacgct tcgccggcat ccgtcaacgt ggcctgatgg tatacgggct tgatcccgag
     1921 catatccatg atctgcatgg tgacgaaggt gctcccgcca ccggccggtc cggggttcca
     1981 cttcttcccg tccagatcct tcagggtctt gatgttggcg tccttggcga cggcaaactg
     2041 ccacacggcg ggggcaaagg ggaagattgt ccggagatcg tgcttgtcct tgaaatcccg
     2101 cttgaccagg gcggcgtcgt agttggccat gtccgccttc ttgttctgga gcatatgcat
     2161 gctctcttcg gtgcccgcgg tgtccaccac ggtgatccgc agaccggtgt cattggcgat
     2221 gatggtccat gtgagggccg cccacatgta ctgacccgct gtcgaaggcc atccgccaaa
     2281 gaaatactcc cgagtctgcc agtcagcctt caacccggcc gtgccagcct gggctgacgg
     2341 cgaactcagc acgaccgcaa ggcccaccaa cgccacgagc accagcagcc gccacttccc
     2401 ggttttcatt ttggagccct ccttcctgat gtatcaggcg ataccgcagg ggcagtgtct
     2461 acgtccctcg acttgccagc ctacgccgac ggggcgccgg ccgccggttt cctgtacttc
     2521 cgcagactcc ccgccttcat ga
//
LOCUS       DBNGZC010001073         5007 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_191851, whole genome shotgun sequence.
ACCESSION   DBNGZC010001073 DBNGZC010000000
VERSION     DBNGZC010001073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_191851"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="AC16G0_18530"
     CDS             complement(<1..861)
                     /locus_tag="AC16G0_18530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HIR5101657.1"
                     /translation="MGTEILTIGTELLLGQIVDTNASWISQRLAEAGIDVFYKTTVGD
                     NWGRIEAALRLAMDRADAIIITGGLGPTEDDLTRDVLAAVLARPLKLDPAVLQHIQER
                     FTRRKITMTENNRKQAMVPEGAEVLHNPRGTAPGLLLKHQGRVLVCMPGVPAEMKPML
                     AEQVIPRIREAFGIKGRIVSRVLKACGISESKLDHLIGDYFREMRNPTIGVLAHAGEI
                     HVRLTCKGDDPEDITRKLDDLEAKIRARLGHLIFGRDDEKLEALVGRLLRERKATLAV
                     AESFTGGVIAN"
     gene            complement(958..1458)
                     /locus_tag="AC16G0_18535"
     CDS             complement(958..1458)
                     /locus_tag="AC16G0_18535"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF016489.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A family protein"
                     /protein_id="HIR5101658.1"
                     /translation="MATRSSAEASQGHGRIGPAQRLLATGAGVGYLPFAPGTAGSLLG
                     AALCVPLLFLPEPVYLGATVVLTAMAVWAAGGMALELGIADPAPVVIDEIVGMLWAAI
                     ALRPHPYDVTVAFLLFRVFDVVKPVPIPWLERLPGGLGIVADDVAAGLLARVLWWLLQ
                     VNFDFL"
     gene            complement(1461..2837)
                     /gene="rimO"
                     /locus_tag="AC16G0_18540"
     CDS             complement(1461..2837)
                     /gene="rimO"
                     /locus_tag="AC16G0_18540"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR5101659.1"
                     /translation="MPKRTKTDPAKPLTVSLVSLGCPKNQVDAEVMLGQLAAAGYRVA
                     EAADQADLVVVNTCSFIQEAKEESIRTVLEAADLKRTGRCRAVVVSGCLAQRYGQELP
                     VLLPEVDAFVGTGEFPRIADIAHRVLHRPREERVWVTGHSALVTAELPRRLLTPGHYA
                     YLKVSEGCDRTCSFCAIPAIRGRMQSRTREDILAEARRLADSGVREVILVSQETTAYG
                     ADRGEPHGLVSLLTALLGIPSLRWIRLHYLYPTQVTPELIGLLAAEPRLCRYVDLPLQ
                     HSQGPILKAMRRGGDARSLRRLVSRIREAVPDVTLRTSFITGFPGETRAHFQGLLAFV
                     KEMRFDRLGVFRYSDEEGTAAAALHPKVPRRVAEARRARLMAVQSRIAEEKGRALIGS
                     IQEVLVDGPAEGFPGLVCGRTAAHAPEVDGTIYLYGPAVPAGSFVRARIREAFEHDLS
                     GEILGLTG"
     gene            complement(2930..4885)
                     /locus_tag="AC16G0_18545"
     CDS             complement(2930..4885)
                     /locus_tag="AC16G0_18545"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIR5101660.1"
                     /translation="MSAVTNDSRSTILVVEDEVGIARMIQVLLEARGFVALVSHSGDD
                     AFDRLASQTVDLVLLDLMMPGMDGYEVCRRLKADPRWSHIPVVMLTAKDTVRDKILGL
                     EIGADDYITKPFNTDELVARINVLLRIQAMSQELVRRNRELQALNAVALAVNRSLSLE
                     EILEGALQRTLENLRLETGLVHLVEPKTGHLVLAAWRGLPTGSLAAFSRLLPGTGWAW
                     QAMAQQEALVVTDATREESLGALAFKPHQPLVGIPLRLRDRELGVLTVIGHPGQPLSA
                     EDIGLLEAIGRQVAIALENAHLYADSRRKAREFEGLNQIGRAMAATFNLQEILTRISE
                     AVSNLFHARAMSLMLLSSDGQTLSTVAGSNLFDEKPHAEQSQGARQGSSPSFVAVLEK
                     RPVAAPDLFADEAYAQWLQAARDNGYRSFLAIPLIVQERALGCMNLYLTEPHEFDNDE
                     IQILSTFASQAAISIENARLFEEARQLAITDELTGLANHRQFYQQLTREVQRAERYDR
                     PLTLLMIDVDFFKEYNDRYGHLAGDHALRDLAYVMRCNARNVDILARYGGEEFAIILP
                     ETDLNRAAFQAERIRAAVAAHPFRSNDPAFRGVLTVSIGVTTLAPGIQKIEELVHDAD
                     QAMYLAKTRGRNRVEMAPIRGRSADST"
BASE COUNT          838 a         1700 c         1646 g          823 t
ORIGIN      
        1 gttggcgatg acgcctcccg tgaaggattc ggccacggcc agcgtggcct ttcgctcccg
       61 gagcaggcgg ccgaccagcg cctccagttt ctcgtcgtcc cgaccgaaga tgaggtgacc
      121 cagtcgcgcc cggatcttcg cctccagatc gtccagcttt cgtgtgatgt cctcgggatc
      181 atcccccttg caggtcaggc gcacgtggat ctccccggcg tgcgccagga ccccgatggt
      241 gggattacgc atctcgcgga agtagtcccc gatgaggtga tccagcttcg actcgctgat
      301 cccgcaggcc ttgaggaccc gggacacgat ccggcccttg atgccgaagg cctcccggat
      361 ccgggggatc acttgctcag ccagcatggg cttcatctcg gccgggacgc cgggcatgca
      421 gaccaagacg cgcccctgat gcttcagcag gaggcccggg gccgtgccgc gcggattgtg
      481 cagcacctcg gccccttccg ggaccatggc ttgcttgcgg ttgttctccg tcatcgtgat
      541 cttgcggcgg gtgaagcgtt cttgaatgtg ctgcaggacg gccgggtcca gcttgagcgg
      601 ccgcgccagg acggcggcca ggacgtcgcg ggtcagatca tcctccgtcg gtcccaggcc
      661 cccggtgatg atgatggcgt cggctcggtc catggcgagg cgaagcgccg cctcgatccg
      721 gccccagtta tcccccacgg tcgtcttgta gaagacatcg atgcccgcct cggccagccg
      781 ctgcgagatc cagctcgcgt tggtatccac gatctgcccg agcagcaact cggtcccaat
      841 cgtcagaatc tctgtcccca tgtcagttca ctccagggag ccctccgcaa tttcgaaatt
      901 cgaatttcga atcgacagga aggccatccg actgtccggc ccgcgagcgc gcgcccctca
      961 cagaaaatcg aagttcacct gcagcagcca ccacagcact cgggccagga gaccggcggc
     1021 cacgtcgtcc gccacgatgc ccagcccgcc gggcagccgc tccagccagg ggatggggac
     1081 gggcttcacc acgtcgaaga cccgaaacag gagaaacgcg acggtgacgt cgtaggggtg
     1141 cggccgcagg gcgatggcgg cccacagcat cccgacgatc tcgtctatca cgaccggcgc
     1201 cggatccgct atgcccagct ccagcgccat cccgcccgcc gcccacaccg ccatcgccgt
     1261 gaggacgacc gtcgcgccga gatacacggg ttcgggtagg aagagcaggg ggacgcagag
     1321 ggcggcgcca agcaggctgc ccgcggtgcc cggggcgaag ggcagatacc cgacacccgc
     1381 cccggtcgcc aagaggcgct gggccggacc gatccgcccg tgcccctggg aggcctcggc
     1441 ggacgagcgc gttgccatgg ctaccccgtg agccccagga tctcgcccga gaggtcatgc
     1501 tcgaaggctt cccggatgcg ggcccgcacg aagctcccag caggaaccgc cggcccgtag
     1561 agatagatcg tgccgtccac ctcgggcgcg tgagccgccg tccggccgca gacgagaccc
     1621 gggaatccct cggcggggcc gtccaccagg acctcctgga tgctgccgat cagggcacgt
     1681 cccttttcct cggcgatccg cgattgaacc gccatgaggc gggcccgccg ggcctcggcc
     1741 acgcgccggg gcaccttggg atggagcgcc gccgccgcgg tgccctcctc gtcagaatag
     1801 cggaagaccc ccagccgatc gaaccgcatc tccttcacga aggccagcag gccttggaag
     1861 tgggcccgcg tctcaccggg aaaccccgtg atgaaggagg tgcggagcgt cacatcgggc
     1921 acggcctcac gaatacgcga gaccagccgg cggaggctac gcgcatcgcc cccgcgcctc
     1981 atggccttga ggatcggccc ctggctgtgc tgcagcggca gatcgacgta acggcagagc
     2041 cgcggctccg ccgccagcag gccgatcaac tcgggagtca cctgggtggg atagaggtag
     2101 tgaagacgga tccagcgaag tgacgggatt cccaggagcg ccgtgagcag cgagacgagc
     2161 ccgtgcggct ctccccggtc cgccccgtag gcggtcgtct cctgggaaac caggatgacc
     2221 tcccggacgc cggagtccgc gagacgcctg gcctcggcca ggatatcctc ccgcgtccga
     2281 ctctgcatcc ggccgcggat ggcggggatc gcgcagaagc tgcaggtgcg gtcgcagccc
     2341 tcggagacct tgaggtaggc gtaatgaccc ggcgtgagca ggcgccgtgg cagttcggcc
     2401 gtcaccaggg ccgagtgccc cgtcacccac acccgctcct cccgcggtcg atggagcacc
     2461 cgatgggcga tgtccgcgat ccgtggaaac tcccccgttc cgacgaaggc atccacctcg
     2521 ggaaggagga ctggcagttc ctggccgtag cgctgggcca ggcatccgct caccaccacg
     2581 gcccggcagc ggccggtccg cttcaaatcc gcggcctcca ggacggtccg gatggattcc
     2641 tccttggcct cctggatgaa gctgcaggtg ttcacgacca ccagatcggc ctggtccgcc
     2701 gcctcggcca cccgataccc cgccgccgcg agctgaccca gcatcacctc ggcgtccacc
     2761 tggttcttgg gacacccgag gctcaccagg ctgaccgtga gcggtttcgc gggatctgtc
     2821 ttcgtgcgtt tcggcatgca gcatcctcgg ttatcgccga ccgccagacg cgatcttcat
     2881 ggcgagcgtg gaagggaagg ggatggatga ccagggatac cggaatggtt tacgtgctgt
     2941 ccgctgagcg gccccggatc ggcgccatct ctactcggtt gcggccgcgg gtcttggcga
     3001 ggtacatggc ctggtcggca tcgtgcacca gctcttcgat cttctgaatc ccgggggcca
     3061 gggtcgtcac gccgatgctc accgtcagca caccccggaa ggcggggtcg ttgctccgga
     3121 agggatgcgc ggccaccgca gcccggatgc gctcggcctg gaacgccgcc cggttcaggt
     3181 cagtttccgg gaggatgatg gcgaattcct cgcccccgta ccgagccagg atgtccacgt
     3241 tgcgggcgtt gcaccgcatg acgtaggcca gatcgcgcag ggcgtgatcc ccggccaggt
     3301 gcccatagcg gtcattgtac tccttgaaga agtccacatc gatcatgagc agggtcaggg
     3361 gccggtcgta ccgctcggcg cgctggacct cgcgcgtcaa ctgctggtag aactggcggt
     3421 ggttggccag gccggtcaac tcatcggtga tggcgagctg ccgcgcctcc tcgaacagtc
     3481 tggcgttctc gatggagatg gcggcctggc tggcgaaggt ggagagaatc tggatctcat
     3541 cgttgtcgaa ttcgtggggc tcggtgagat acaggttcat gcagcccaga gcgcgctcct
     3601 ggacgatgag cgggatggcc aggaaggacc ggtatccatt gtctcgggcc gcctgtagcc
     3661 actgcgcgta ggcttcatct gcgaaaaggt cgggggccgc gaccgggcgc ttctccagca
     3721 cggcgacgaa gcttgggctg cttccctgcc gtgcgccctg gctctgctcc gcgtgtggct
     3781 tctcgtcgaa aaggttggac ccggccaccg tggagagggt ctgcccgtcg gagctgagga
     3841 gcatgaggga catggcccgg gcgtggaaca ggttggacac ggcctcgctg atccgcgtca
     3901 ggatctcttg gaggttgaag gttgcggcca tggcccgacc gatctgattc aagccttcga
     3961 actcccgggc cttgcgccga ctgtcggcat acaggtgggc gttctccagg gcgatggcca
     4021 cctgccgccc gatggcctcg agcaaaccga tgtcctccgc ggagagcggt tggccgggat
     4081 gccctatgac ggtgagaacg cccagctccc gatcgcgcaa gcggaggggg atgcccacca
     4141 gcggctggtg gggcttgaag gccaacgccc ccaaactctc ctcccgcgtc gcatccgtga
     4201 cgaccagggc ctcctgctgt gccatggcct gccaggccca tcccgtcccc gggaggagac
     4261 gggaaaacgc cgccaaggac cccgtcggta gtccccgcca ggccgccagg accaggtgtc
     4321 cggtcttggg ctccaccagg tgaacgagac cggtctccag ccgcaaattc tccagcgttc
     4381 tctgcagggc gccttccagt atttcctcca ggctgagcga gcgattcacc gccagggcca
     4441 ccgcattcag cgcctgcaac tcgcggttgc gacggaccaa ttcctggctc atggcctgga
     4501 tccggagaag cacattgatg cgggccacga gttcgtccgt gttgaagggc ttggtgatgt
     4561 agtcgtcagc cccgatctcc aggcccagga tcttgtcccg caccgtatcc ttggccgtga
     4621 gcatcaccac gggaatgtgg gaccagcgcg ggtcggcctt cagacggcga cagacctcat
     4681 agccatccat ccccggcatc atcagatcca agagcacgag atccacggtc tgggaggcca
     4741 ggcggtcgaa ggcgtcgtcc cccgagtggc tcaccagcgc cacaaacccg cgcgcctcca
     4801 gcaggacctg gatcatgcga gcgatcccga cttcgtcctc cacgacgagg atggtggatc
     4861 gggagtcgtt cgtgacagca gacatcgtcg gctcgccttt ccttcgccaa tcgaccacgc
     4921 gcctcaagga tgggttcttt tcagcagcct gcgcaaatac gagtttgtca gcaggctgtt
     4981 aagcggctgc gggcaccttt gggatgg
//
LOCUS       DBNGZC010001074         2009 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_679930, whole genome shotgun sequence.
ACCESSION   DBNGZC010001074 DBNGZC010000000
VERSION     DBNGZC010001074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_679930"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="AC16G0_18550"
     CDS             complement(<1..190)
                     /locus_tag="AC16G0_18550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIR5101661.1"
                     /translation="MENAKLQLAVGTFVLLGLMCLGYLSIKLGKLEVVGGQAYEIQAE
                     FDSASGLKPGAAVEIAGVE"
     gene            complement(200..985)
                     /locus_tag="AC16G0_18555"
     CDS             complement(200..985)
                     /locus_tag="AC16G0_18555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5101662.1"
                     /translation="MRGVNLTIPAGKLTTIIGRSGEGKSVLLKHIIGLMRPDRGEVWV
                     DGLEISRLTGKRLNQVRERFAMLFQGAALFDSLTVAENVAFPLREKRRLPEPEVSRRV
                     EAKLDQVGLSGMGHKYPAELSGGMMKRAGLARALVMEPEIILFDEPTTGLDPLMAKAI
                     RELIVATQRAVGFTAVMVSHDIPQVFAISDWVAMLRGGTIAAMAPPAAFQQTADPDIR
                     EFISVDNPFVRQARHGRRNGNGNGRSDTSLLYSHTLQSLYAGR"
     gene            complement(1105..1875)
                     /locus_tag="AC16G0_18560"
     CDS             complement(1105..1875)
                     /locus_tag="AC16G0_18560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family ABC transporter permease"
                     /protein_id="HIR5101663.1"
                     /translation="MTTAVTRIGRITLALIQEMGRMLIFLLATFTWMVRPPHRPVQMV
                     RQLNFIGVKSTLVVVLTAGFTGMVLGLQLQYTLQKFGSEAVLGSVVGLSIIRELGPVF
                     AALMVTARAGSAMTAEIGLMQITEQVDALETMAISPLQYLIAPKLVACVIGVPLLVAI
                     FDVVGISGGYLVGVKLLGGNAGSYWGSLGSAVEWKDVSGGILKSVSFGLLVSWVCCYK
                     GYYSRPSAEGLGKATTEAVVLSSILILVSDYFLTSVLL"
BASE COUNT          393 a          685 c          564 g          367 t
ORIGIN      
        1 cctccacccc cgcaatctcc acggcggccc ccggcttcag ccccgacgcc gaatcgaact
       61 cagcctgaat ttcgtacgcc tgccccccga ccacctccag cttccccagc ttgatggaca
      121 ggtagccgag acacatgagg cccaagagca cgaaggttcc gactgctagc tgtagcttgg
      181 cattctccat ggccgtcctc tatcgtccag cataaagaga ttgcagcgta tgactgtaca
      241 ggagggaggt gtcagacctt ccgttcccat tcccattcct gcgcccatgc cgggcctgcc
      301 tcacgaaagg gttgtcgacg ctgatgaact cacggatgtc cggatcagcg gtctgctgga
      361 atgccgccgg cggggccatg gccgcgatgg tgccacctcg gagcatggcc acccaatccg
      421 aaatggcgaa gacttgcgga atgtcgtgac tcaccatcac cgccgtaaac ccgacggccc
      481 gctgtgtggc cacaatcaat tcccggatgg ccttggccat cagcgggtcc agtcccgtgg
      541 ttggctcatc aaacaaaatg atttctggtt ccatcaccag cgcccgggcg agtccggccc
      601 gcttcatcat ccccccgctc agttcggccg ggtacttatg ccccatgccc gagaggccta
      661 cctgatccag ctttgcttca acccgacggc tcacctccgg ctcgggcagg cggcgcttct
      721 cgcgcaaggg aaaggccacg ttctccgcga cggtcaggga atcgaacagg gcggcgccct
      781 ggaagagcat ggcaaaccgc tcgcgtacct ggttcaggcg tttccccgtc aggcgtgaga
      841 tctccagccc gtccacccac acctcgccac ggtcgggccg catcaggccg atgatatgtt
      901 tgagcaggac gctcttgccc tcgccgctcc ggccgatgat ggtggtgagc ttacccgccg
      961 gaatggtgag gtttacccca cgcaagaccg gttgcccccc gagcgttttc tcgactccca
     1021 caagtttgat cggcgacgga gcacccgtcc ttgccaagcc ccgagcgact tgttgaggca
     1081 gaatccgtaa tctgctgatg ttcatcatag gagcaccgac gtcaggaaat aatccgagac
     1141 caggatgaga atcgacgaca ggaccaccgc ttcggtggtg gctttcccta acccctccgc
     1201 gctcggtctg ctgtagtacc ccttgtagca gcacacccag cttacgagca gcccgaaact
     1261 caccgacttc aggatgcctc cagacacgtc cttccactcc accgccgatc ccagagaccc
     1321 ccagtaggag cctgcatttc ccccaaggag ctttacgccg accaggtaac cgccggagat
     1381 gcccaccacg tcgaagatcg ccaccaagag tggcacgccg atcacgcagg cgacgagttt
     1441 gggggcgatc aggtactgca gcgggctgat cgccatggtc tccagggcgt ccacctgctc
     1501 ggtgatctgc atgaggccga tctcggccgt catggctgag ccggcccgcg ccgtcaccat
     1561 caaggcggcg aacaccggcc ccagctctcg gatgatgctg agccccacca cggatccgag
     1621 gaccgcctcg gacccaaact tctggagcgt gtattggagt tgtaggccca gcaccatgcc
     1681 ggtgaagccg gccgtcagga ctacgaccag cgtggatttg accccgatga aattcagttg
     1741 cctgaccatt tgcacaggtc gatggggcgg ccgcaccatc catgtgaagg tggccagcag
     1801 gaagatcagc atccgtccca tttcctgaat cagggccaga gtgatgcggc cgatgcgtgt
     1861 aacagcagtg gtcaacacct gtgcacctat gtcagcggcc ggataacatg gcggcctgct
     1921 cccggtcgcc ccgaccgcta tcctcacgaa cggcatgctc tcgccgctgc agatacgcat
     1981 tgcggacggc gctgtacatg tcgacggtg
//
LOCUS       DBNGZC010001075         1956 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_484165, whole genome shotgun sequence.
ACCESSION   DBNGZC010001075 DBNGZC010000000
VERSION     DBNGZC010001075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_484165"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1879)
                     /locus_tag="AC16G0_18565"
     CDS             complement(113..1879)
                     /locus_tag="AC16G0_18565"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5101664.1"
                     /translation="MGAENLEALLRATRSVMSGLDLPGILQRIVAEASHMARAPHVAV
                     LLLDREAQVLRVAALAGNPVPPGFEIPLGTDLSGVVAQTGESVFSPNSSTDPRNLLAE
                     RDRENGLVTYLGLPIKGREGILGVLTFETTTPRRYTQDELAYLTSFADNAAMAIENAW
                     LHRTVQEQAARLEARVQERTTALEEALRAKAEFLARMSHELRTPLNFILGFADLLHQG
                     IGGALTAKQVQFVDRIRTGGKHLLDLVHDILELSLVEGGKRGLRLEKIPLAPLVQEVL
                     DIFSIQALQKRLCVVTSLEPGLCIVVERRRLLQILSNLLGNAMKFTPEGGTITLTARH
                     VKSSWSRQVDAPRHVASSTTRPDNISDACIEIAVKDTGIGIPAADIERIFRGFEQVDG
                     STSRRFGGAGIGLSLVRTLVELHGGRVWAESAGPGTGTRFVVQLPLLPEPSPKRIVVV
                     EDDVAIAEALAALLRNEGYGVEGANTGAEGLAAILANPPDLILLDIGLPDMDGREVLK
                     RLRAGERTHSLPILVLTGLGEDLGEGALALGADEFLTKPISASVLTRIVGEILRGGVP
                     KPQDVFPVQTHDEPGHEGGGTG"
BASE COUNT          358 a          666 c          577 g          355 t
ORIGIN      
        1 ccagaatgcg ttccgacatg tttggctccc tcttgaccgg gttgtccccc accgcgaata
       61 cccttcgagc ccccgagtca ccgcgagggg gcattcacct tgcgagaagc catcaccctg
      121 ttccgccgcc ttcgtggccc ggttcgtcat gcgtctgaac cgggaaaacg tcctgcggct
      181 tggggacgcc ccctctcagg atctctccca caatccgggt gaggacgctg gccgagattg
      241 gcttggtcag gaactcgtcg gcccccaagg cgagagcccc ctcacccaga tcttcaccca
      301 gccccgtgag aacgaggatg ggcaggctgt gcgtccgttc ccctgcacgg aggcgcttga
      361 gcacctcccg gccgtccata tccgggagac cgatatcgag caagatcaga tccggcggat
      421 tcgccaggat tgcggcgagc ccctccgctc ccgtattcgc cccttccaca ccatagccct
      481 cattccggag gagggcagcc aacgcctccg cgatggcgac atcatcttcc accaccacga
      541 tccgcttcgg tgatggctcc gggagcagcg ggagttgcac cacgaagcgc gtgccggtgc
      601 ccggcccggc actctccgcc cagacccgac ctccgtgcag ctcgaccagg gtgcgaacca
      661 aggacaaacc aattcctgcc ccgccgaagc gtcgactcgt tgagccatcg acttgctcga
      721 aaccgcggaa gatccgctcg atatctgcag cagggatgcc gatgccggtg tccttgactg
      781 cgatctcgat gcatgcatca ctgatgttat ccggacgtgt cgtcgatgaa gcgacgtgtc
      841 ggggcgcgtc gacctgacgg ctccaggaac tcttgacatg tcgtgccgtg agggtgatcg
      901 tgcccccttc gggggtgaac ttcatggcgt ttcccagcag attggagagt atctggagca
      961 acctgcgccg ctccacgaca atgcaaagtc ccggctccag cgatgtgaca acgcagagac
     1021 gcttctgcaa ggcctggatg ctgaagatgt ccagcacctc ttgcaccagg ggcgccaagg
     1081 gaatcttctc caggcggagg cctcgctttc ccccctcgac cagggataat tccaggatgt
     1141 cgtggacgag atccagcagg tgcttgcctc ccgtacggat acggtcgaca aactggactt
     1201 gcttcgccgt caacgccccg ccgatgccct ggtgcaggag gtccgcaaag cccaggatga
     1261 agttcagggg tgtcctgagc tcgtgcgaca tccttgccag gaactcggcc ttcgcccgga
     1321 gcgcctcttc cagggcggtg gtccgctcct ggactcgggc ttccaaccgg gcagcctgct
     1381 cttggaccgt gcgatgcagc caggcgttct cgatcgccat ggccgcgttg tcggcaaagg
     1441 aggtcagata cgccagttcg tcttgggtgt agcggcgggg cgtggtggtc tcgaaggtca
     1501 acacgccgag aatcccctcc cggcccttaa tgggaagacc gagatacgtg accaacccat
     1561 tttcccgatc ccgttcggct aggaggtttc gcggatccgt ggaggagttc ggactgaaaa
     1621 cggactctcc cgtttgcgcc acgacgccag acagatcggt ccccagcgga atctcaaacc
     1681 ctggcgggac ggggttcccc gccagcgccg ccacccggag gacctgcgcc tccctgtcca
     1741 acagaagcac ggctacgtgg ggtgccctgg ccatgtggga agcttccgcc acaattcgct
     1801 gcaggattcc cgggagatcc aatcccgaca tgacggatcg tgtcgcccgc aggagcgcct
     1861 ccagattctc cgcgcccatc atcgccccga acctctcaag ggcagcagga cggtaaagac
     1921 gcttccacta ccctccccgg acgactcggc ccagat
//
LOCUS       DBNGZC010001076         2303 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1857183, whole genome shotgun sequence.
ACCESSION   DBNGZC010001076 DBNGZC010000000
VERSION     DBNGZC010001076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1857183"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="AC16G0_18570"
     CDS             <1..1040
                     /locus_tag="AC16G0_18570"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR5101665.1"
                     /translation="GLDTVFFCNSGAEAVDGALKFAHAFTRRKRFLYCDHGFHGLTLG
                     TLAVSGCREFRADYEEILTAGTEIPFGDSARLETEISRGDVAAFVVEPVVGHGVFIPP
                     HDFLPRARELCTRHGTLLIADEVQTGFGRTGKLFACEHWNVVPDIMTISKSLSGGYCP
                     IGATLYPRKVYDKVFSSLDRCMVHSTTFGQNDLAAVCGLATLEVIREEGLVENSQRMG
                     AYLLDGLKRLGEKYELIQEVRGLGLMIAIEFGQPKSLTLKAGWKMLHGLNQDLFCQSI
                     LVPLIMDHRILAQVSSHGRDIIKLIPPLVFDQADADRFIGAFDQVLAATHRLPGPIWE
                     VGSRLAKAALR"
     gene            1070..2185
                     /locus_tag="AC16G0_18575"
     CDS             1070..2185
                     /locus_tag="AC16G0_18575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HIR5101666.1"
                     /translation="MNPLRDPEQGRSGIPTPQSALRGILGFRAAYGGLAVGILVFAAV
                     LAWQGLDDVTAALRQAGWGIIAVAILHLPQIWADAMGWRRLVPSGQQPPRRTMIWARW
                     IGESINDLLPVLQMGGNIVKAWILANRGVRIGPAGASVVVDVTLVVLTQILFTLFGVS
                     VLVPSLGGGRALLVVLVGAAIMTALLAGFYAVQRRGFFGLLVRMSRRILGGPEWASFS
                     DGAATMDADILRQYGDRRVLMTSGCWHMLAWFVGIAEVWFALYLLGQPVNVRTALLFE
                     SLGQAIRTGAFAVPGALGIQEGGYVLVGSALGIGPGVALGLSLVRRVRELLLGIPGLA
                     SWQATTICRAFRKRAVSPGTDRPDQSADLSPQRKEIS"
BASE COUNT          349 a          737 c          785 g          432 t
ORIGIN      
        1 cgggtctcga cacggtcttc ttttgcaact ccggggcaga ggccgtcgat ggcgccctca
       61 agttcgcgca cgccttcacc aggaggaaga ggttcctcta ctgtgaccac gggtttcacg
      121 gcctgacact cggtaccctg gccgtaagcg gatgcaggga gttccgggct gactacgagg
      181 aaatcctgac tgctgggacg gagatcccct ttggcgactc cgcgcggctg gagaccgaga
      241 tctcgcgagg cgacgtggcc gcgttcgtgg tggagcccgt tgtcggccac ggcgtgttca
      301 ttccccccca cgactttctc ccgcgggcgc gggagctttg cacccgccac ggcaccctgc
      361 tgatcgccga tgaagtccag accggtttcg gccgcacggg caagcttttc gcctgcgagc
      421 actggaacgt ggtccccgac atcatgacca tctcgaagtc cctgtcgggc gggtactgtc
      481 ccatcggcgc caccctttac ccgcggaagg tctacgacaa ggtcttcagc tccctggacc
      541 ggtgcatggt gcactccacg acattcgggc agaatgacct ggccgcggtc tgtggtctcg
      601 ccaccctcga ggtgatccga gaggaaggcc tcgtcgagaa cagccaacgg atgggggcgt
      661 acctcctgga cggcctgaag cgcctcggtg agaaatacga actcatccag gaggtgcggg
      721 gcctgggcct catgatcgcc atcgagttcg ggcagccaaa atcgctgacc ctgaaggcgg
      781 gctggaagat gctccacgga ctcaaccagg atctcttctg ccagtcaatt ctggtgcccc
      841 tgatcatgga ccaccgcatc ctggcccagg tctccagcca cggcagggac atcatcaagc
      901 tgatcccccc cctggtcttc gaccaggccg acgcagaccg gttcatcggg gccttcgacc
      961 aggtcctggc cgcaacgcac cggctaccgg ggccgatctg ggaggtcggc tcccgcctgg
     1021 cgaaggcggc tctgcgctag atgcgacggg ctgccggcag gctcgagtca tgaatcccct
     1081 tcgcgatccg gagcagggac ggtctgggat cccgacccca cagagcgccc tccgcgggat
     1141 cctcggtttc cgcgcggcat acggcggcct ggcggtgggc atcctggtgt tcgctgccgt
     1201 cctcgcctgg cagggattgg acgacgtgac ggcggccctg cggcaggcgg gatggggaat
     1261 catcgcggtc gcgatcctcc atctccctca gatctgggct gacgccatgg gctggcggag
     1321 gctggtcccg tcggggcagc agccgccgcg ccggacgatg atctgggctc gttggatcgg
     1381 cgagtcgatc aacgacctgc ttcccgtcct ccaaatgggt ggaaacatcg tgaaggcatg
     1441 gatcctcgcg aatcgggggg tccgtatcgg gccggccggc gccagcgtcg tggttgacgt
     1501 caccctcgtt gtgctgacgc agatcctctt caccctcttc ggtgtgagcg tcttggtccc
     1561 gagcctcggc ggtgggagag ccctgctggt cgtcctggta ggggccgcca tcatgacggc
     1621 gctcctggcg gggttctacg cggtccagcg gcggggattc ttcggcctcc tcgttcgcat
     1681 gtcgaggagg atcctggggg gaccggaatg ggcctccttt tccgacgggg ccgccacgat
     1741 ggacgcggat atccttcggc agtatggaga ccgccgggtg ttgatgacgt cggggtgctg
     1801 gcacatgctc gcctggtttg tcgggatcgc cgaggtgtgg ttcgcgctct accttctggg
     1861 tcagcctgtc aatgtccgca cggcgttgct tttcgagagc ctgggtcagg caatccggac
     1921 cggggcgttt gcggttccgg gtgcgctcgg cattcaggag gggggctacg tcctggtggg
     1981 cagcgccctc ggcatagggc ccggcgtggc tcttggcctg tcactggtcc ggagggtgcg
     2041 ggaactgctg cttggcatcc ccgggctggc ctcttggcaa gcgacgacca tctgccgggc
     2101 cttccggaaa cgcgcggtga gcccaggcac cgacaggccg gatcagtcgg ccgacctgtc
     2161 tccgcagcgc aaggagatat cgtgagccgg gaaattccag agacctggaa ggtggaccgt
     2221 tcccccctgg tgcatgccat caacgcaggg ggccgactgc tcggccgacg ggcgatgggc
     2281 attttccggg cggagaacct gct
//
LOCUS       DBNGZC010001077         7902 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2441904, whole genome shotgun sequence.
ACCESSION   DBNGZC010001077 DBNGZC010000000
VERSION     DBNGZC010001077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7902)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7902)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7902
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2441904"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /gene="acpS"
                     /locus_tag="AC16G0_18580"
     CDS             complement(<1..279)
                     /gene="acpS"
                     /locus_tag="AC16G0_18580"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIR5101667.1"
                     /translation="MILGLGTDLVAVSRVEEILARHQERFLHRVFTRAEQAECLGRAR
                     PARHLAARLAAKEAAMKALGTGWGLGVRWQDIEVRSGGKTPPALRLGGQ"
     gene            complement(332..1075)
                     /locus_tag="AC16G0_18585"
     CDS             complement(332..1075)
                     /locus_tag="AC16G0_18585"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF003625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HIR5101668.1"
                     /translation="MTILFAGLDHIAGLRQAGGGRDPDPTVAAALAELAGAGGISVSF
                     GRDRAGVQERDLRLLREVSRTAVNVCLPPREEFVKLALDVRPDVVTLIPEGDEGPAGE
                     RGLDVEDRRSELAPVIQTLRAAGIGVSVLVDPVPAQVKAAHRVGVDAVWLFTGGLSWA
                     GDLAVRVAEFEGLVNAAKVGHKLRLAVHAGGGLGYQSVGSVSHVLEIEAVHVGHSLVA
                     RASLVGMAEAVRELLRAMEGPSGLRTPAA"
     gene            complement(1104..2453)
                     /gene="glmM"
                     /locus_tag="AC16G0_18590"
     CDS             complement(1104..2453)
                     /gene="glmM"
                     /locus_tag="AC16G0_18590"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR5101669.1"
                     /translation="MPRLFGTDGVRGIANQEPMTPETVVKLGRAAAYLFKAPGSRRSF
                     VIGKDTRLTGYMLETALTAGITSMGVDVLLVGPLPTPGIAFITRSLRADAGVVISASH
                     NSFEDNGIKFFSGEGLKLPDAIETQVEELILSGEIDRIRPGANEIGKAYRIGDAGGRY
                     IEFAKNTFPKALTLRGLKIVVDCAHGAAYRVSPAVLQELGAEVIALNVAPDGLNINAG
                     CGSLFPAVAQQAVVKEGAHIGFTHDGDGDRVLCVDEKGALVDGDQVLTICALDMLAEG
                     RLRGHAVVATVMSNLGLEVAMRENGIKVVRTPVGDRYVLEEMLRSGFSLGGEQSGHII
                     FGEHNTTGDGIVTALQVLAAMLRAKQPLSELAARMPCFPQVLENVRVQRKQELGTLPG
                     VQAKIREAEAALGDSGRVLVRYSGTEPLVRVMIEGPDEKTIRRWAGEIVAAIRTTLG"
     gene            complement(2531..3199)
                     /locus_tag="AC16G0_18595"
     CDS             complement(2531..3199)
                     /locus_tag="AC16G0_18595"
                     /inference="COORDINATES: protein motif:HMM:NF019561.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIR5101670.1"
                     /translation="MGRIERGMWTWLLDNIGLKLLSLILAAFLWVVVLGEQKVEVSVN
                     IPLEYTLPKNLILVNDPPDNLEVHLRGPKTLATTLAPREVVLSSVPSKFAEGENIITI
                     NRDNIRAPRGIDVLQVTPHRIRLVLEAVVEREVEVKPRLEGAPADGFLVKAVTSSPAR
                     VKLVGPRSELRRLTQVSTLPIRLDGQAASFSTRVMLEPVGGEVHVRGDTPISVQVEIT
                     PKKS"
     gene            complement(3296..4141)
                     /gene="cdaA"
                     /locus_tag="AC16G0_18600"
     CDS             complement(3296..4141)
                     /gene="cdaA"
                     /locus_tag="AC16G0_18600"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013162682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HIR5101671.1"
                     /translation="MLDLIHQFRWIDALDILIVAFAIYQIILALRGTRAFQMLLGLAL
                     LYVASWVSLRIGLITVNWALSNLLAVWFLLIIILFQPELRRALASVGRRGSLLRAFSR
                     YQEAHMVDEVVRAVASLAAKKIGAIVVVERDSRLSDTVDSGTAVDAMVSRRLLESIFF
                     PYSPLHDGAVIISRDRIVAAGCFLPLSINPHLPRDLGTRHRAAVGVTEETDAIAIVVS
                     EETGAISLVMGGEIAQHLDAASLRQRLGGVLGPPLAAGRPVKGEAPSSLQELANPHGN
                     GPQRL"
     gene            complement(4437..6254)
                     /gene="ftsH"
                     /locus_tag="AC16G0_18605"
     CDS             complement(4437..6254)
                     /gene="ftsH"
                     /locus_tag="AC16G0_18605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR5101672.1"
                     /translation="MSPFFKNLALWLVIGLIMVLVFNIFNQTQPMQKELIFSDFMNKV
                     ERGEVAEVTIRGNDVTGRLSDNTVFRTYTPDDKDMIPVLRKQGVKITAKPAEGQPWYM
                     SVLVSWLPMLLFIGVWVFFMRQMQGGGAKALSFGKSRARLLSDKQNKVTFADVSGVDE
                     AKEELQEIIEFLKDPQKFQKLGGRIPKGVLLMGPPGTGKTLLARAIAGEANVPFFSIS
                     GSDFVEMFVGVGASRVRDLFEQGKKNAPCIIFMDEIDAVGRHRGAGLGGGHDEREQTL
                     NQLLVEMDGFESNDGVILIAATNRPDVLDPALLRPGRFDRQVVVARPDVKGREGILKV
                     HTRKIPLGPDVDLQVLARGTPGFSGADLANLVNEGALLAARANKKLVEMSDFENAKDK
                     VLMGVERKSLVISAKERKITAYHEAGHALVAKLLPGTDPIHKVTIIPRGRALGVTQQL
                     PTEDKHNYSKEFLQNDIAILYGGRVAEEIVFGTQEITTGAMNDLERATELSRKMVCEW
                     GMSERMGPLQFGKREEMIFLGREIAQHQDYSEQTAMEIDREVRRIVMENYERAKDLIQ
                     RRLETLHTLAEALLEREVLDGPEIDGIVKGAGSPAPAAA"
     gene            complement(6447..7028)
                     /gene="hpt"
                     /locus_tag="AC16G0_18610"
     CDS             complement(6447..7028)
                     /gene="hpt"
                     /locus_tag="AC16G0_18610"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR5101673.1"
                     /translation="MQYRIGPTLISATDIQTRIRELATAISADYAGRDLLLICVLKGA
                     AIFWADLCRSLSIPCQSDFVAMESYGNSTSPLEEPRFTKAADVALSNRDILIVEDIID
                     TGHTGVRLRALLQSQSPRSLAMCSLLDKAERRQVAVPIEYCGFVIPNAFVVGYGLDYA
                     QKYRHLPQIATLEALPGEGPPLQSLDDGAEPLA"
     gene            complement(6988..>7902)
                     /gene="tilS"
                     /locus_tag="AC16G0_18615"
     CDS             complement(6988..>7902)
                     /gene="tilS"
                     /locus_tag="AC16G0_18615"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF023162.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR5101674.1"
                     /translation="VVFREDASNASDAYRRNRIRHHLLPLLAKEYNPRIARSLAALAS
                     LVREDEAALATMAGGLLAAGARTVGRGIRLDLARSRSAPPAVARRALQEAFRLASQEV
                     HGLTRRHLEALLGLLTRDAVVQLPGGLVATRIGDAVEIGPRPMSDGVRSRRAASAWNA
                     FPEVSVRPGRWVSWDPLGCRVRVRRVTAGAVPSASQGGRQEILSPTVLAGSLSLRAWQ
                     PGDRFRPLGLRGQKKLQDFFVDAKVPRGQRARIPLLLADGRIAWVVGQRVAEDFRWAG
                     RGTACLAEVEFQEETDAVPDRPDPHQRD"
BASE COUNT         1342 a         2810 c         2479 g         1271 t
ORIGIN      
        1 ctggccgccc agccggagcg cgggcggggt cttccctccc gaccgcacct cgatgtcctg
       61 ccaccgcacg cccagccccc aacccgtccc cagggccttc atggccgcct ccttggcggc
      121 caggcgagcc gccagatgcc tggcgggacg cgcccgtccc agacactcgg cctgttccgc
      181 gcgggtgaac acccggtgca ggaagcgctc ctggtgccgc gcgagaatct cctccacacg
      241 cgagacagcg accaaatcgg tgcccagccc cagaatcatg tggcgtctct cagggcagtc
      301 ggaggctctt ggccttcacc catggccgcc atcacgccgc aggagtccgg aggccggatg
      361 gaccctccat ggcccgaagc aactcccgca ccgcctcagc catgcccacc aggctggctc
      421 gggcgaccag gctgtgaccg acgtggaccg cctcgatctc cagaacgtgg ctgaccgaac
      481 ccacgctctg gtaccccagg ccgccgcccg cgtgcaccgc gagccggagc ttgtgcccga
      541 ccttggccgc attcaccagg ccctcgaact ctgcgacccg gacggcaaga tcaccggccc
      601 aggagaggcc cccggtgaac agccagactg catccacccc gacccggtgc gcggccttga
      661 cctgcgccgg cacgggatcc accaggacgc tgaccccgat ccccgccgcc cggagcgtct
      721 ggatgaccgg tgccagctcg gagcgacgat cctccacgtc caggccccgc tcccccgccg
      781 gaccctcgtc accttcaggg atcagcgtga ccacatccgg tcggacgtcc agcgccagct
      841 tcacgaactc ctcgcgcggc ggcaggcaca cgttcacggc cgtccgcgac acctcgcgaa
      901 gcagccgcag gtcccgctcc tgcactccgg cccggtcgcg gccgaagctg acgctgatcc
      961 cgcctgcacc ggccaactcc gccaaggcgg cggcgaccgt ggggtccgga tcccgcccgc
     1021 ctcctgcctg tcgcaggccg gcgatatgat ccagaccggc aaacaggatg gtcatggcgt
     1081 cacctcacct cgcggctcgc agctcatccc agggtagtcc ggatcgccgc cacgatctcc
     1141 ccggcccagc gacggatcgt cttctcgtcc ggcccctcga tcatgacccg gaccagcggc
     1201 tcagtgccgg agtagcgcac cagaaccctc cccgagtccc ccagggccgc ctcggcctcg
     1261 cgaatcttgg cctggacccc cgggagcgtc ccgagttcct gcttccgctg cacccgcaca
     1321 ttctccagca cctgggggaa gcagggcatg cgcgccgcca gctcggagag gggctgcttg
     1381 gcccgcagca tcgccgccag gacctgcaag gcggtcacga tcccgtcccc cgtggtgttg
     1441 tgctcgccga agatgatgtg ccccgactgc tcaccaccca ggctgaaacc ggagcggagc
     1501 atctcctcca gcacgtagcg atcccccacc ggcgtccgca ccaccttgat cccgttctcc
     1561 cgcatggcca cttccaggcc gaggttgctc atgaccgtgg ccacgacggc atgcccccgg
     1621 agacgtccct ccgccagcat gtccagggcg cagatggtca ggacctggtc cccgtccacc
     1681 agggctccct tttcatccac gcacaggacg cgatcgccgt ccccgtcgtg ggtgaaaccg
     1741 atgtgggccc cctccttcac caccgcctgc tgtgccaccg cggggaagag cgacccgcaa
     1801 cccgcgttga tgttcaggcc gtccggcgcc acgttcaggg caatcacctc tgcgcccaac
     1861 tcctgcagga cggcgggaga gacccggtag gcggctccgt gagcgcagtc caccacgatc
     1921 ttgagcccgc gcagtgtcag ggccttggga aacgtgttct ttgcaaactc gatgtaccgg
     1981 ccgcccgcgt cgccaatgcg gtaggccttc ccgatctcgt tggccccagg gcggatgcga
     2041 tcaatctccc cgctcaggat cagctcctcc acctgggtct caatcgcatc gggcagcttc
     2101 aggccctccc ccgagaagaa cttgataccg ttatcctcga aggagttgtg cgaggcggag
     2161 atgacgaccc cggcgtcggc gcgcaggctc cgcgtgatga aggcgatgcc cggcgtgggc
     2221 agcgggccca ccaggaggac gtccacgccc atggaggtga tcccggcggt gagggccgtc
     2281 tccagcatgt agccggtcag ccgcgtgtcc ttgccgatca cgaagctccg gcggctgccc
     2341 ggcgccttga acagataggc ggcggcccgg cccagcttga cgaccgtctc gggcgtcatg
     2401 ggctcctgat tggcgatacc ccggactcca tccgtcccga acaagcgtgg catgcctgtc
     2461 acctcccgac aaccatcggc cgcccgaggc cgcaggacca cccccgggtc gtctgacgga
     2521 tcaagccagt tcaggatttc ttcggcgtga tctcgacctg cacgctgatg ggcgtgtcgc
     2581 cccggacgtg gacctcaccc ccgaccggtt ccagcatcac ccgcgtcgag aaggaggccg
     2641 cctgcccatc cagccggatc ggcagggtgg acacctgcgt gagtcgtcgc agttcgctcc
     2701 tcggaccaac caacttgacc cgggccgggc tggaggtgac ggctttcacg aggaagccat
     2761 cggcaggcgc accctccagt cgcggcttca cctccacctc ccgctccacc actgcctcca
     2821 ggaccagccg gatccggtgc ggggtgacct gcagcacatc gatcccccgc ggggcgcgga
     2881 tattatcccg gttgatcgtg atgatgttct ctccctccgc gaacttgctg gggacgctgc
     2941 tcaagacaac ctcacgcggc gccaaggtgg tcgccagggt cttgggtccg cgcaggtgga
     3001 cctccaggtt gtcgggagga tcgttcacca ggatgaggtt cttaggcagg gtgtactcca
     3061 ggggaatgtt cacgctgacc tcgaccttct gctcccccag gaccacgacc cacaggaatg
     3121 ccgccaggat gagggacaag agctttagac cgatgttatc caggagccag gtccacatgc
     3181 cccgctcgat tcgccccatc gtgcccgctg ccgctgcttg atcgcgcgcc gcccggccac
     3241 tttagcagtc ggaagcttga tgctatcaca cacccggagc agtgcgcacc cgttctcaga
     3301 gccgctgcgg cccgtttccg tgcgggttgg ccaactcctg aagcgaggaa ggcgcctcgc
     3361 ctttcaccgg ccggccggca gccaggggtg gacccagcac gccgccgagt cgctgccgga
     3421 ggctagccgc atccaggtgc tgggcgatct cgccgcccat gaccagagag atcgcccccg
     3481 tttcctcgga gaccacgatg gcaatggcgt cggtctcctc ggtcacgccg acggcggccc
     3541 gatgccgcgt ccccagatcg cgtggcagat gggggttgat gctgagcggc aggaagcagc
     3601 cagccgccac gatgcggtcg cggctgatga tgaccgcccc gtcgtggagc ggcgagtagg
     3661 ggaagaagat ggactccagg aggcggcggg agaccatggc gtccaccgcc gtgcctgaat
     3721 ccacggtgtc ggagagacgc gaatcccgtt ccaccacgac gatggccccg atcttcttgg
     3781 cggccagcga ggccacggcc cgcacgactt cgtcgaccat gtgggcttcc tgataccggg
     3841 agaacgcccg caggaggctg ccccgccggc cgacgctggc cagcgcccgg cgcagttccg
     3901 gttggaacag gatgatgatg agcaggaacc agacggccag caggttgctc agggcccagt
     3961 tcacggtgat gaggccgatg cgcaaagaca cccaggacgc gacgtagaga agcgccaacc
     4021 ccaggagcat ctggaacgcc cgcgttccac ggagcgccag gatgatctgg tagatggcaa
     4081 aggccacgat caggatatcc agggcgtcga tccaccggaa ctggtgaatc agatccagca
     4141 tccccttaca ccccctgggg cgcgccgggc cggcacggat ccgacaccag cggtgtgccg
     4201 tcacggccaa gtgacgcaga cggatgcggg aggaagcgcg ggaagggcag gctgctgcgg
     4261 gcggacgcgg ccccggcgag cttgctagcc ggccggcccg ccggactcat caggggggaa
     4321 gctgccgtcg gtccggtctg agcgatgggg accccctgat ccatgtgcca tccggtccac
     4381 tgttctgtga ctcaccatcc gggcaaacaa ccgcccggcc gaaccccaca ccggagctag
     4441 gccgccgcgg gcgcggggga gccggccccc ttcacgatgc catcgatctc cgggccgtcc
     4501 aagacctcgc gctccaagag ggcctcggcc agggtatgca gggtctcgag gcggcgctgg
     4561 atgagatcct tggctcgctc gtagttctcc atgacgatgc ggcggacctc gcggtcgatc
     4621 tccatcgccg tctgctcgct gtaatcctgg tgctgcgcga tctcgcgacc caggaagatc
     4681 atctcctcgc gcttgccgaa ctgcaggggg cccatgcgct cactcatccc ccactcgcag
     4741 accatcttcc gcgacagctc ggtggcccgc tccagatcgt tcatggcgcc tgtggtgatc
     4801 tcctgggtgc caaagacgat ctcttcagcc acccggcccc cgtagaggat ggcgatgtcg
     4861 ttctgcagaa attccttgga gtaattgtgc ttgtcctcgg tgggcaactg ttgcgtgacg
     4921 cccaaggcac gaccccgcgg gatgatggtg accttgtgga tgggatccgt gcccggcagg
     4981 agcttggcca ccagcgcatg gccggcctcg tggtaggcgg tgattttccg ttccttggcg
     5041 ctgatcacca ggctcttccg ctccacgccc atgaggacct tgtccttggc gttctcgaaa
     5101 tccgacatct cgaccagctt cttgttcgcc cgggccgcca ggagcgcccc ctcgttcacc
     5161 aggttggcca ggtccgcccc cgagaagccc ggcgtgccgc gcgccaagac ctggagatcc
     5221 acgtccgggc ccagggggat cttccgggtg tgcaccttca ggatcccttc gcggcccttc
     5281 acgtccggcc gcgccacgac cacttgccga tcgaaccgcc caggccgcag cagcgccggg
     5341 tccaggacat ccggccggtt cgtggccgcg atgaggatga cgccgtcatt gctctcgaac
     5401 ccgtccattt ccaccaggag ctggttcagg gtctgctccc gctcgtcgtg gccgcccccc
     5461 aaccccgcac cccggtgacg cccaacggcg tcgatctcgt ccatgaagat gatgcagggg
     5521 gcgttcttct tcccctgctc gaagagatcc cggacgcggg aggcgcccac gcccacgaac
     5581 atctccacga aatcggagcc ggagatggag aagaagggca cgttggcctc gccggcgatg
     5641 gcccgggcca aaagggtttt gcccgtgccg ggaggaccca tgagcagcac gcctttcggg
     5701 atgcggcccc ccagcttctg gaacttctgg ggatctttga ggaactcgat gatctcctgg
     5761 agttcttcct tggcttcgtc cacgccggac acatcggcaa aggtcacctt gttctgctta
     5821 tccgagagca ggcgtgcccg actcttgccg aaggacaatg ccttggcgcc cccgccctgc
     5881 atctgcctca tgaagaagac ccacaccccg atgaacagga gcatggggag ccaggaaacg
     5941 aggacgctca tgtaccaggg ctgcccctcg gcgggcttgg ccgtgatctt gacgccctgt
     6001 ttgcgcagga ccgggatcat gtccttgtcg tcgggcgtgt aggtccggaa aaccgtgtta
     6061 tcggaaagcc gacccgtgac gtcattgccc cggatcgtga cctcggcgac ctcgccgcgc
     6121 tccaccttgt tcatgaagtc gctgaagatc agctccttct gcatcggctg ggtctggttg
     6181 aagatgttga acaccagcac catgatgagg ccgatgacca gccataaggc aagattcttg
     6241 aaaaacgggc tcacccgaat tctcctccat caggtcgcac gcgcggccgt ttattcgttg
     6301 ggcttttgta ccacgaaagt ccgcgataca caacaggaaa tgtgagattt cgaccgtcgg
     6361 aaactgcgta catcatcgcc agtgagaaag atctgcccag gaatccacgg agtctaccgc
     6421 gggagagcat gctccccccg ccccgttcag gcgagaggct cggcgccgtc gtccagtgat
     6481 tggagcggag gcccctctcc ggggagcgcc tccagcgttg cgatctgggg caggtgccga
     6541 tacttctgcg cgtagtccag gccgtacccc acgacaaagg cattgggaat cacgaagccg
     6601 caatactcga tgggaaccgc gacctggcgg cgctccgcct tgtccagcag gctgcacatg
     6661 gccagactgc gcggcgactg gctctggagc agcgctcgca gacggacgcc ggtgtggccg
     6721 gtgtcgatga tgtcctccac gatcagaatg tcccggttgg acagggcgac atctgccgcc
     6781 ttggtgaagc gaggctcttc caggggcgag gtggaattgc cgtagctctc catggccacg
     6841 aagtcgctct ggcaggggat gctgagactg cggcagaggt ccgcccaaaa gatcgccgcc
     6901 cctttcaaga cgcagatcag caagaggtcc cggcccgcgt agtctgcgga gatcgccgtg
     6961 gccagctccc ggatgcgggt ctggatgtca gtcgcgctga tgagggtcgg gccgatccgg
     7021 tactgcatcc gtctcctcct gaaattcgac ttccgcaagg caggccgtgc ccctgcctgc
     7081 ccagcggaaa tcctcggcga cccgctgacc caccacccat gcgatccgtc catcggcaag
     7141 gaggaggggt atcctggcgc gctgcccgcg gggaaccttg gcatcaacga agaagtcctg
     7201 gagctttttc tgcccgcgga gacccaaagg ccgaaaccga tccccgggtt gccatgcccg
     7261 caagctgagg gagccggcca gcacggttgg actcaggatc tcctgacggc ctccctgcga
     7321 ggcagacggg acggcgcccg ccgtcacccg gcggacacgc acgcggcagc cgagcgggtc
     7381 ccagctcacc cacctgccgg gtcggacgga aacctcggga aacgcattcc aggctgaggc
     7441 ggcccgtcga gatcggacac catcggacat tggccgcggc ccgatctcca cggcgtcgcc
     7501 gatccgcgtc gccaccagac ccccgggaag ctgcaccacg gcatcccgcg tgagcaggcc
     7561 gagaagggcc tcgaggtgcc gccgcgtcaa gccgtgtacc tcctggcttg ccaggcggaa
     7621 ggcctcctga agcgctcgac gcgccacggc cgggggggcg gagcgagatc gcgcgaggtc
     7681 gagccggatg ccccttccca ccgttcgggc ccctgcggcc aggagacccc cggccatggt
     7741 tgccagcgcc gcctcatcct cgcgcacgag gctcgccaat gcggccagcg acctcgcgat
     7801 tcgtgggttg tactctttgg ccagcagggg gaggagatga tgccggatcc ggttccggcg
     7861 atacgcgtcg gaagcgttgg acgcatcctc acggaagacc ac
//
LOCUS       DBNGZC010001078         1785 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_877401, whole genome shotgun sequence.
ACCESSION   DBNGZC010001078 DBNGZC010000000
VERSION     DBNGZC010001078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_877401"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..565)
                     /locus_tag="AC16G0_18620"
     CDS             complement(65..565)
                     /locus_tag="AC16G0_18620"
                     /inference="COORDINATES: protein motif:HMM:TIGR01462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HIR5101675.1"
                     /translation="MVTNNIVDRLKEKLASLEREFRVDLPKRIQHARELGDLRESGEY
                     ETAKDRQGFLNARIGFLQRRIAELSSIDLSRVPRDRVAFGSTVHLVEEETGQEQTYRL
                     VASEEVDSSQGWISPGSPVGQGLIGKREGDLIEIRTPNGARRYEVVKVITLHDELAQE
                     AEETNG"
     gene            complement(711..>1785)
                     /gene="gltX"
                     /locus_tag="AC16G0_18625"
     CDS             complement(711..>1785)
                     /gene="gltX"
                     /locus_tag="AC16G0_18625"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR5101676.1"
                     /translation="RAAKQSLRHLCPCRLQGPAPGRRQAIRFHVARAGKVIWDDLVQG
                     RIEVAVEEIDDFIILRSDEGETPVYNFAAAVDDAMMGITHVVRGADHIPNTPKQILLY
                     EALGYTLPAFGHIPLVLGTDREKLSKRHGDVALHAYREQGYLPEAMVNYLVRLGWASG
                     DQEVFTREEIIRAFRLERVGASPGIFDRAKLDWLNAFHLRQTPPETLADLLSPFWEPV
                     GVSRAAVESRGRDWLAQALPLFVERSRTLLELARSMVFLFASPREHDPKAAAKFLDLE
                     HLGYLREVRLALAEAPDFSTEALEASVRKLADARGKKLVEYAQPIRVALTGTTVSPPI
                     FPVMVLLGREVVLDRLAEVLGPN"
BASE COUNT          279 a          635 c          562 g          309 t
ORIGIN      
        1 cagggcgtgc ttgagcaggc tttcgttctt ggtgtactcg gtgagcagct cccatgtggc
       61 ctgcctatcc attcgtctcc tccgcctcct gtgccagctc gtcgtgcagc gtgatcacct
      121 tcaccacctc gtatcgccgc gccccgttgg gtgtccggat ctcgatcagg tccccctccc
      181 gcttcccgat gaggccctgg cccacgggcg agccggggga gatccagccc tgcgagctgt
      241 ccacctcctc cgaggcgacc aggcggtacg tctgctcctg tcccgtctct tcctccacca
      301 gatggaccgt ggacccgaag gcgactcgat cgcgggggac ccgcgacaga tcgatgctcg
      361 acagctcggc gatgcgccgc tgaaggaatc cgatccgcgc attcaggaag ccctgtcgat
      421 ccttggcggt ctcgtactcg ccgctctccc gcaggtcgcc cagctcgcgg gcgtgctgga
      481 tgcgcttggg gaggtccaca cggaattccc gctccaggct ggccagcttc tccttcagcc
      541 gatccacgat gttgttcgtt accacctctc acctcccaat catcacaaag gttcgaccgt
      601 tcgaggctgc gagcgttcgg cacgccccag gcagtcgaac ggtccctcgg ttgatcgttg
      661 cttgcgacgg agcgcccggg cgggcggcgt cccgcgtccc gcccgcgccg tcagttgggg
      721 ccgagcacct cggccagacg atccaggact acctcgcgcc ccagaaggac catgacagga
      781 aagatgggcg ggctgacggt ggtcccggtc agggccaccc ggatcggctg cgcgtattcc
      841 acgagcttct tgccgcgcgc gtcggcaagc ttccgcaccg aagcctccaa cgcctccgta
      901 ctgaaatccg gcgcctcggc cagtgccaga cggacctcgc gcaggtaccc gagatgctcc
      961 aggtccagaa acttggctgc ggccttgggg tcgtgctcac gcggcgaggc gaagaggaac
     1021 accatggagc gcgccagctc caggagagtt cgggaccgct ccacgaacag cggcaatgct
     1081 tgcgcgagcc agtccctccc tcgggattcg accgccgccc gcgacacccc gaccggctcc
     1141 cagaaaggcg acagaagatc cgccaacgtt tctggcggcg tttgccgcag gtggaacgcg
     1201 ttgagccagt ccagcttggc ccggtcgaag atccccggcg acgcgccgac tcgctccaag
     1261 cggaaggccc ggatgatctc ctcccgcgtg aatacctcct ggtctccgga ggcccagccc
     1321 agtcgcacga ggtagttgac catggcctcc ggcaggtagc cctgctcccg gtaggcgtgc
     1381 agtgccacgt ccccgtgccg cttggaaagc ttctcccggt cggtcccgag gaccaggggg
     1441 atgtgcccga aggcaggcag cgtgtatccc agggcctcgt acaggaggat ctgcttcggc
     1501 gtgttcggga tgtggtccgc accccgcacg acgtgagtga tgcccatcat ggcatcgtcc
     1561 acggcggcgg caaagttata gacgggggtc tcgccctcgt ccgatctgag gatgatgaag
     1621 tcgtcgatct cctccacggc gacctcgatc cgtccttgca ccagatcgtc ccagatgacc
     1681 ttccccgccc gcgccacgtg gaaccggatg gcctgccgcc gtccaggcgc ggggccctgc
     1741 aggcggcacg ggcagaggtg gcgcagcgac tgcttggcag cccgg
//
LOCUS       DBNGZC010001079         1726 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2346757, whole genome shotgun sequence.
ACCESSION   DBNGZC010001079 DBNGZC010000000
VERSION     DBNGZC010001079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2346757"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..554
                     /gene="lspA"
                     /locus_tag="AC16G0_18630"
     CDS             75..554
                     /gene="lspA"
                     /locus_tag="AC16G0_18630"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR5101677.1"
                     /translation="MSFYLIALGVLLLDQATKQVIIRSLRLGEGFPVVPGYFDITFVL
                     NPGAAFSFLATLSERVRNPFFIAISLAAIVLIVVYHSRFAHQHRLASFALALVLGGAT
                     GNLVDRLRYGVVVDFLDLHIHQYHWPVFNVADSAISVGVSLMLLEMLLDWRDGKRSE"
     gene            584..1561
                     /locus_tag="AC16G0_18635"
     CDS             584..1561
                     /locus_tag="AC16G0_18635"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR5101678.1"
                     /translation="MADQLHEYRVAERDAGQRLDAFLARASGLSRARVQRLIQDGHVL
                     LGGHPQKSHHRVSPGETIQLRVPPPTPLDLTPEAIPLDILYEDEDILVLNKPAGMVVH
                     PGAGRATGTLVHALLAHCQELPGIGGVERPGIVHRLDRDTSGGLIIAKTEAAHLSLSR
                     QFKARQVRKTYLALVHGEVRQEKGRIEAAIGRRENDRKRMGVRTRGGREARTAYRVLR
                     RLPGMTLLELDLETGRTHQIRVHLAHIAHPVVGDEVYGGRRERRHAGLDAQPAKRQLL
                     HAWRLGFHHPRTGAWMEFTAPVPEDLLSLAGLPRLFPSPTAGQSAESPA"
BASE COUNT          279 a          573 c          582 g          292 t
ORIGIN      
        1 tgcaggcagg ctagggcgca aggaccgatg cacagattcc agcgaccgat gcgggatgac
       61 cgaagagcat cgtcatgagc ttttacctca ttgccctggg cgtcctcctc ttggaccagg
      121 ccacaaagca ggtcatcatc cgaagtctcc ggctggggga ggggtttccc gtggtccccg
      181 gctactttga catcaccttc gtgctgaatc cgggtgcggc cttcagcttc ctggccacgc
      241 tctcggagcg ggtgcggaac cccttcttca tcgccatttc cctcgcggcg atcgtcttga
      301 tcgtcgtgta tcattcgcgt ttcgcccatc agcatcgcct cgcgtccttc gccttggccc
      361 tggtcctggg gggggccacg ggcaacctcg tggatcggct gcgctacggt gtggtggtgg
      421 acttcctgga tctccacatc caccagtatc actggccggt cttcaacgtc gcagactccg
      481 ccatctccgt gggggtcagc ctgatgctcc tggagatgct gctcgactgg cgtgacggga
      541 agcgatcgga atagccgttg gccgcgaaag gcggacagcg ccaatggctg accaactgca
      601 cgagtaccgg gttgccgagc gtgatgcggg gcagcgcctg gacgcgttcc tcgcccgggc
      661 gagcggatta tcgcgcgccc gggtgcagcg actcatccag gacgggcacg tccttctggg
      721 cgggcacccg cagaagtctc accaccgggt gagtccgggc gagacgatcc agcttcgcgt
      781 gccgcccccc acgcccctcg acctcacgcc ggaagccatc cccctcgaca tcctctacga
      841 agacgaggac atcctcgtcc tgaacaagcc ggccggcatg gtggtccatc cgggcgccgg
      901 gagggccacc gggaccctgg tccacgcact cctggcccac tgccaggagc tgcccggtat
      961 tggcggcgtc gagcggcccg gcatcgttca ccggctggac cgagacacct ccggcggcct
     1021 catcatcgcc aagacggagg ccgcacacct atccctcagc cggcagttca aggcgcgcca
     1081 ggtgcggaag acgtacctgg cgctggtcca cggcgaggtg cgtcaggaga aggggcggat
     1141 cgaggcggcg atcggccggc gagaaaacga ccggaagcga atgggcgtcc ggacgagagg
     1201 cggacgcgag gcccgtaccg cctaccgcgt gcttcggcgg ctgccgggca tgacgctgct
     1261 cgagctggac ctggagaccg gacggaccca ccagatccga gttcacctgg cccacatcgc
     1321 tcacccagtg gtgggtgatg aggtgtacgg agggcggcga gaacgccgac acgccgggtt
     1381 ggacgctcag ccggcgaagc gccaactgct tcacgcctgg cgtctgggat ttcatcatcc
     1441 tcggacgggg gcgtggatgg aattcaccgc gcccgtgccg gaggatctcc tgagtctggc
     1501 aggcttgcct cggctgttcc catccccgac ggccggtcaa tcggctgagt cgcccgcctg
     1561 accgggaggg cgtagaggcg aagacccgga tgcgcagcct ggacacggcc tccgtctttt
     1621 tcagccacat gaggccggga ttgggagagg gtgggtcttt cggacgggtc gcgtccaggg
     1681 gcggccgccg gccatagcgg caaggctcag gcggtcgcta ccgctt
//
LOCUS       DBNGZC010001080         1361 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2536211, whole genome shotgun sequence.
ACCESSION   DBNGZC010001080 DBNGZC010000000
VERSION     DBNGZC010001080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1361)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2536211"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..717
                     /locus_tag="AC16G0_18640"
     CDS             295..717
                     /locus_tag="AC16G0_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101679.1"
                     /translation="MIDCEGGVGADLETIRTVLELLEAHQIRFSSDDGLDLTPEAVLL
                     RLQDPEAFEAWTCGGTVAQLRAWYAHDWKCRAHTRSKRRCKNWVAVVEQGRCYNAPEP
                     GFGELEGFHPHEPAFLFCRLHLSRLTPSDGVNRHRRPS"
BASE COUNT          238 a          448 c          453 g          222 t
ORIGIN      
        1 gatgcttcgc accgccaccg gcgttccgtt tcgtggtcga atcccgtccc gtcgaacacc
       61 gcgagcggga gccggaggag gaggtaacgg gaggtgtgac ggacggttgt cggggatttg
      121 cgggcgaccc gactaccggg caatgaggta caaagttata ctaacgggca atgggggatg
      181 gttggggccc cgaccaccga tcatcccgga ggcactttga ggagaccagc cgggcacttt
      241 cgggagacga accaggtact ttcgggagac gaagggaccg gcgggaggcg agggatgatc
      301 gactgcgaag ggggagtcgg cgcggatctc gagacgatcc gcacggtcct ggagttgctc
      361 gaggcgcatc agatccggtt ttccagcgac gacggtctcg acctgacgcc ggaggcggtc
      421 ctcttgcgcc tccaggaccc cgaagccttc gaggcgtgga cctgcggcgg tacggtggcc
      481 caactccgcg cctggtatgc ccacgattgg aagtgccggg cccacacccg gtcgaagcgg
      541 cggtgcaaga actgggtggc ggtcgtggag cagggccgtt gctacaacgc ccccgagcca
      601 ggattcggcg aattagaggg cttccacccg cacgagcccg cgttcctgtt ctgccgattg
      661 cacctgtcgc gactcacccc gtctgatggt gtgaacagac accgccggcc ctcttgagga
      721 gaaaggccgg tcgatctcgc gggtttggac cgaggtgatg gacctgccga gggtggaccg
      781 ggtgccgatg atccgagcgg cgccgccggt gccgaccctc gggccatccg gcgtcccgat
      841 gccggggcgc gtcgccacga ggaaggaccg agttgaggtc ctactcccgc ctgctcggct
      901 cgacatcatc cattgggcca gaccactgcc aaacaggctc atcaggccac cgaccgccgt
      961 cccaacaacc tggatccaag gattcacgtc agccatctcg ccccctccag aacgtcggcc
     1021 accggatagg cacacggccg ctgatatttc ccatcagcgt aacagaaccc gggttacccc
     1081 gacgttcccc gcgcagcccg caggccatgg cacgcccccc ttgcgcgcgt cgtgggccgg
     1141 cttgctccac ccccgcctca gttcgcgacg gggttgccgg acaggcgagg cgggcgttgg
     1201 accatctggc ttcttgccgt cgtctgggag agccgaagat tgacgacatc attcgttttg
     1261 acgcaccgca accatgaaga tcgccccgcg gttcctcgcc ttcggaccca tcgcctggtc
     1321 atacgctgag aggagccggg ccgggttcag ctcgccacgc c
//
LOCUS       DBNGZC010001081         4625 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2150510, whole genome shotgun sequence.
ACCESSION   DBNGZC010001081 DBNGZC010000000
VERSION     DBNGZC010001081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2150510"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1683
                     /locus_tag="AC16G0_18645"
     CDS             <1..1683
                     /locus_tag="AC16G0_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5101680.1"
                     /translation="SWVNQDKQAATQKAKDLVRAAVHRVGFHKALVRKQVPINPKVLV
                     VGGGIAGIHAALTLANAGKQVYLVEREPTIGGHMAMFDKTFPTLDCAACILTPKMSAV
                     KTHPNITLWTYSEVVGVDGYVGNYKIKVKRKPRYIKEDLCVGCMECVTACTFKVGRFP
                     DEFNQGLSKRKPVYIPFPQATPQLVLFDPNACIQLKTGKCKQSCVEACDRKAIDFKQI
                     AETEEIEVGTVILATGFQTFDATRLPYYGYGIYPNVYTSLEVERLINASGPTGGEVVL
                     RDGRKPEAVGIVHCVGSRDEKTNRWCSRVCCMYSLKLAHLVKEHTDAKIYNFYIDMRT
                     PGKSYEEFYDKLLKEGVHFIRGRVAEVTDWARTSEEEGKLVVRVEDTLVGTVRRIPLD
                     MVILSVGLEPRADAQEVRRLFNISCSTEGFFLERHPKLAPVNTFTDGIFIAGACQGPR
                     DIPDAVAQAGAAAAEALVLIDTGHVELEPNTAYIVEEDCSGCKSCVPLCPYTAIKFSE
                     EKKKASINEALCKGCGTCVAACPSGSIRQNLFEDEQIFSEINGLLSELAQVS"
     gene            1705..2235
                     /locus_tag="AC16G0_18650"
     CDS             1705..2235
                     /locus_tag="AC16G0_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HIR5101681.1"
                     /translation="MPTQKPHNGDGTPASWEPRIVAFFCNWCTYTAADLAGVSRMKHA
                     PNVRIIRVMCSGRVDPQFVLDAFAKGADGVLIGGCHIGDCHYVEGNYKAVRRVQMLRR
                     ILQGLGIQDDRFRLEWISASEGEKVKLVINDMAEKVRALGPLGLPQRFQEWDQEMESL
                     QHTVEAELEEVGAHAR"
     gene            2225..3205
                     /locus_tag="AC16G0_18655"
     CDS             2225..3205
                     /locus_tag="AC16G0_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIR5101682.1"
                     /translation="MPAKPKVAFYWCASCGGCEESVVDLAEGILGVVEAVDIIFWPVA
                     MDFKRRDVEAMADGAILASLINGAVRTTEQEEMVRLLRRKSQLVIAYGACAQLGGIPG
                     LANQFSREQILKYAYEESPSTQNAEKAYPTPQFQDNGRTATLPGLWKIVRTLDQVIPV
                     DYYLPGCPPTPKLLQQALTALLSGAAPPKGSVLAPDIALCEECPRKETKPTDLSFPAF
                     KRPHTSEVDPVKCLLAQGFLCMGAATRGGCEALCVKGNMPCSGCFGPTSRVKDQGAKF
                     MSSLCSNIAATDEKEIDSTLATIPDPVGTFYRYGLAASLLRARVRRQPEA"
     gene            3214..>4625
                     /locus_tag="AC16G0_18660"
     CDS             3214..>4625
                     /locus_tag="AC16G0_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HIR5101683.1"
                     /translation="MQTRTISIDPITRLEGHGKIDIFLDAKGEVERAYFQVPELRGFE
                     IFSIGRPAEDMPQITSRICGVCPTAHHMAGTKALDDLYNVEPPPTAKRIRELVYNTFM
                     LEDHALHVYILSGPDFIVGPTAPKELRNVVGVIQKVGVDVGKKVIGMRRRLRELIAYF
                     GGKVIHPVLGLPGGVSKGLSPDDLPGFKQLAEDGLEFARFTLQVFKDIVLKNPEYVKL
                     VTSDTYTHKTYYMGMVDEHNRVNFYDGKIRVVDPLGKEFTSFPADHYLANIAEHVEPW
                     SYVKFCYLKSLGWKGFVDGPDSSVYSVAPLARLNAADGMATPEAQKAYEEYFATLGGK
                     PVHFTLANHWARVIEMLYAAERMRELVNAPDITGKNIRTIPTATPKVGIGVVEAPRGT
                     LFHHYETDEQGLIKKANLIVATQGNSARMAMGIEKSAKGLIHAGRVDDGLLNMIEMAF
                     RAYDPCHGCATHSLPGEMPL"
BASE COUNT          900 a         1490 c         1452 g          783 t
ORIGIN      
        1 tcgtgggtga atcaggacaa gcaggccgcc acccagaagg ccaaggatct ggtcagggcg
       61 gctgtgcacc gggtgggctt ccacaaagcc ctggtgcgca agcaagtccc catcaacccc
      121 aaggttctgg tggtcggagg cggcattgcg ggcatccacg ccgccctgac cttggccaac
      181 gccggcaaac aggtctatct cgtcgagcgg gagcccacca tcggtggcca catggccatg
      241 ttcgacaaga ccttccccac gctcgactgc gcggcgtgta tcctcacccc caagatgtcg
      301 gcggtgaaga cccatcccaa catcaccctg tggacgtact cggaagtggt cggcgtagac
      361 ggctacgtcg gcaattacaa gatcaaggtg aagcggaagc cccgctacat caaggaagac
      421 ctgtgcgtgg gctgcatgga gtgcgtgacg gcctgcactt tcaaggtcgg ccgctttccc
      481 gacgagttca accagggtct gagcaagcgc aagccggtct acatcccgtt tccccaggcc
      541 accccgcagc tcgtcctctt cgaccccaat gcctgcatcc agctgaagac cggcaagtgc
      601 aagcagagct gcgtcgaggc gtgcgatcgc aaggcgatcg atttcaagca gatcgcagag
      661 acggaagaga tcgaggtggg cacggtcatc ctggccacgg gcttccagac tttcgatgcc
      721 acccgcctcc cctactacgg atacgggatc taccccaacg tctacacctc cctggaggtc
      781 gaacgcctga tcaatgcctc cgggcccacg gggggggagg tcgttctgcg ggatggccgg
      841 aagcccgagg cggtagggat cgtccactgc gtcggctccc gcgacgagaa gaccaatcgc
      901 tggtgctctc gggtctgctg catgtactcc ctgaaactcg cccacctggt gaaggagcac
      961 acggacgcaa agatctataa tttctacatc gatatgcgga cccccgggaa gagctacgag
     1021 gagttctacg ataagctgct caaggagggc gtccacttca tccggggtcg tgtggccgag
     1081 gtgaccgact gggcgcgaac ctccgaggag gaggggaagc tggtggtccg cgtggaggac
     1141 acgctggtgg ggacggtgcg gcgtatcccc ctggacatgg tgatcctgtc ggttggcctc
     1201 gagccccgag ccgacgctca ggaagtccgg cgcctcttca acatctcctg ctccacggaa
     1261 gggttcttcc tggagcgcca tccaaagctc gcgcccgtga acaccttcac cgacggcatc
     1321 ttcattgccg gggcttgcca gggtccccgc gatatcccgg atgccgtggc gcaggcgggt
     1381 gcggcggcgg ccgaggcctt ggtcctcatt gacaccggcc atgtggagct ggagccgaac
     1441 accgcctaca tcgtcgaaga ggattgctcc gggtgcaagt cctgcgtgcc cctctgcccc
     1501 tatactgcca tcaagttctc ggaagagaag aagaaggcct ccatcaacga agccctgtgc
     1561 aagggctgcg gcacctgcgt ggcggcctgt ccgtcgggct cgatccggca gaatctcttc
     1621 gaggacgaac agatcttcag cgagatcaac ggactgttgt cggaattggc ccaggtctca
     1681 taagggaggc aaaacaccgt gtctctgccg actcaaaaac cacacaacgg ggacgggacg
     1741 ccggcgagct gggagccgcg catcgttgca tttttctgta actggtgcac ctacaccgcc
     1801 gcggatctcg ccggggtttc gcgcatgaag catgcgccca acgtgcgcat catccgggtc
     1861 atgtgctccg ggcgggtgga cccgcagttc gtcctggacg cctttgccaa gggggccgac
     1921 ggggtgctca tcggcggctg ccacatcggc gactgccact acgtcgaagg caactacaag
     1981 gccgtccggc gtgtccagat gctccggcgc atcctgcagg ggttggggat tcaggacgat
     2041 cgcttccgcc tggagtggat ttctgcctcc gagggagaaa aggtgaagct ggtcatcaac
     2101 gacatggcag agaaagtccg ggcgctgggc cccttgggcc tgccccagcg attccaagaa
     2161 tgggaccagg agatggagtc gctgcagcac acagtcgaag cagagctgga ggaggtcggc
     2221 gctcatgccc gctaagccga aggttgcgtt ctactggtgc gcctcctgcg ggggctgcga
     2281 ggaatccgtg gtggacctgg ccgaggggat cctcggcgtc gtggaggccg tggacatcat
     2341 cttctggccg gtggccatgg acttcaagcg ccgcgacgtc gaggccatgg ccgacggcgc
     2401 aatcttggcc agcctgatca atggcgccgt ccgcaccacc gagcaggaag agatggttcg
     2461 tctgctccgc cggaagagcc aactggtcat cgcctatggc gcctgcgccc aactcggcgg
     2521 gatcccgggc ctggcgaacc agttcagccg ggagcagatc ctgaagtacg catacgagga
     2581 atcgccgtcc acccagaacg ccgaaaaggc ctaccccacg ccgcaattcc aggacaatgg
     2641 ccggaccgcc acgcttcccg gactgtggaa gatcgtgcgc acgctggacc aggtgatccc
     2701 ggtggattac tatctgcccg gctgtccgcc gacgccgaag ctcctgcagc aggccctcac
     2761 cgcgctgctc tcgggcgccg cgccgccgaa gggaagcgtc ctcgccccgg atatcgccct
     2821 gtgcgaggag tgcccccgta aagagaccaa gcccacggat ctgtcctttc cggcattcaa
     2881 gcgaccccac acctccgagg tggatccggt caagtgcctt ctggcccagg gattcctgtg
     2941 catgggcgca gcgacccgcg gcggctgcga ggcgctgtgc gtgaagggaa acatgccgtg
     3001 cagcgggtgt ttcggcccga cctcccgtgt caaggatcag ggcgcgaagt tcatgtcctc
     3061 gctctgctcc aacatcgccg ccaccgacga gaaggagatc gactcgacgc tcgccaccat
     3121 cccggacccg gtggggacgt tctaccgtta tggtctcgcg gcttccctcc tccgcgcacg
     3181 cgtgcgccgg cagcccgagg cataggagca gtcatgcaga ctcgaaccat cagcatcgac
     3241 ccgatcaccc gcctggaagg ccacggcaag atcgacatct tcctggacgc caagggggag
     3301 gttgagcgcg cctatttcca ggttcccgag ctacgcggct tcgagatctt cagcatcggc
     3361 cgtcctgccg aggacatgcc ccagatcacc agccggatct gcggcgtttg cccgacagct
     3421 caccacatgg cggggaccaa ggccctggac gacctgtaca atgtcgagcc gccgccgacc
     3481 gcgaagagga ttcgcgagtt ggtctacaac accttcatgc tcgaggatca cgccctccac
     3541 gtgtacatcc tgagcgggcc cgacttcatc gtcggaccca cggcccccaa ggagctgcgg
     3601 aacgtcgtgg gcgtgattca aaaagtcggc gtggacgtcg ggaagaaggt catcggcatg
     3661 cgccgccgcc tacgggagtt gattgcctac ttcggcggga aggtgatcca cccggtcctc
     3721 ggccttcccg ggggcgtgtc caagggactc tcgccggacg acctgcccgg cttcaagcag
     3781 ctcgcggagg acggcctcga attcgcccgg ttcacgctgc aggtcttcaa agacatcgtc
     3841 ctcaagaacc ccgagtacgt gaagctcgtc acctcggata cctacaccca caagacgtac
     3901 tacatgggaa tggtggacga gcacaaccgg gtgaatttct acgacggcaa gatccgcgtg
     3961 gtggatcccc tcggcaagga attcaccagc ttccccgcag accattacct cgccaacatc
     4021 gccgagcacg tggagccctg gagctatgtg aagttctgct acctgaagtc gctgggctgg
     4081 aagggttttg ttgacgggcc cgacagcagc gtctattccg tggccccgtt ggcgcgcctg
     4141 aacgcggccg acggcatggc caccccggag gcccagaagg cgtacgagga atacttcgcc
     4201 acgctgggcg gaaagcccgt gcacttcacg ctcgccaacc actgggcgcg agtcatcgag
     4261 atgctgtacg ccgccgagcg gatgcgggag ctggtgaacg ctcccgatat cacgggcaag
     4321 aacatccgga ccatccccac ggccacaccc aaggtgggca tcggcgtggt cgaagccccg
     4381 cgcgggaccc ttttccatca ttacgagacc gatgaacagg gactgatcaa gaaggccaat
     4441 ctgatcgtgg ccacgcaggg gaactcggcg cggatggcca tgggtatcga gaagagcgcc
     4501 aagggcctca tccacgcggg cagggtggat gacggcctcc taaacatgat cgagatggca
     4561 tttcgagcct acgatccgtg ccatggctgc gccacccact cgcttcccgg cgagatgccg
     4621 ctgat
//
LOCUS       DBNGZC010001082         2726 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2736537, whole genome shotgun sequence.
ACCESSION   DBNGZC010001082 DBNGZC010000000
VERSION     DBNGZC010001082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2736537"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2616
                     /gene="rpoB"
                     /locus_tag="AC16G0_18665"
     CDS             <1..2616
                     /gene="rpoB"
                     /locus_tag="AC16G0_18665"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR5101684.1"
                     /translation="DDIDHLGNRRVRAAGELLEDQFRIGLARMERAVRERMSTQELET
                     LMPHDLVNAKPVTAALKEFFGSSQLSQFMDQTNPLAELTHKRRLSALGPGGLSRERAG
                     FEVRDVHPTHYGRMCPIETPEGPNVGLIASLSTYARVNDFGFIETPYRKVRDGRVGDE
                     IEYLTADEEEKHTIAQANAELDGRGRFVSDRVSARMGGNSVMVTPDTIEYMDVSPKQL
                     VGISTSLIPFLEHDDANRALMGANMQRQAVPLLRPTAPTVGTGMEFVAAKDSGAVVVA
                     KRGGVVESVSAERILVRADPKETRSDGQDNGSGVDIYTLLKFRRSNQNTCINQKPIVA
                     KGDRVMKGQVIADGPATQQGELALGQNVLVAFMPWGGYNFEDAILISERLVKDDRYTS
                     IHIEDFEIEARDTKLGREEITRDIPNVGEEALKDLDESGIIRIGAEVKPGDILVGKVT
                     PKGESVLTPEERLLRAIFGEKAEDVRDASLYVPPGISGTVVDVKILSRKGAEKDERAK
                     SIEDEEVSRLRKDFEDEIQIIQSERDVKIRKQLEDRIVGKDVRGRGSRKVIIPKRHKL
                     TAEVLEGLTTEDMQDVGLQLDDETAAEVTQLIEGAENQVHVLESLLEERIGRVKRGDE
                     LAPGVFKLVKVFVAMKRKLSVGDKIAGRHGNKGVIAKILPEEDMPYLPDGTPVEVVLN
                     PLGVPSRMNVGQVLETHLGWAAAALGLRAASPVFDGASEKDIKLMLKRGNLPPSGKVL
                     LCDGRSGKPFHQEVAVGYIYLMKLAHLVDDKIHARSIGPYSLVTQQPLGGKAQFGGQR
                     FGEMEVWALEAYGAAHTLQEVLTVKSDDVIGRTKMYESIVKGENALEPGLPESFNVLV
                     KELQSLALDVELVKE"
BASE COUNT          539 a          848 c          935 g          404 t
ORIGIN      
        1 gacgacatcg accacctggg caaccgccgc gtgcgggccg cgggggaact cttggaagat
       61 cagttccgca tcggactggc ccggatggag cgagcggtcc gcgagcggat gagcacccag
      121 gagctggaaa ccctgatgcc ccacgacctg gtgaatgcca agccggtgac tgcggcgctg
      181 aaggaattct tcggctcgtc gcagctctcg cagttcatgg accagaccaa cccgctggcc
      241 gagttgaccc acaagcggcg cctctcggcc ctgggcccag gaggcctcag ccgcgagcgg
      301 gccgggttcg aggtccgaga cgttcacccg acacactatg ggcggatgtg ccccatcgag
      361 accccggaag ggcccaacgt gggtctcatc gcctcgctgt ccacctatgc ccgggtgaac
      421 gattttggat tcatcgagac cccctaccgc aaggtgcggg acgggcgggt gggcgatgag
      481 atcgagtacc tcacggcgga cgaggaggag aaacacacca tcgcccaggc caacgccgag
      541 ctggacggcc gcggtcggtt cgtgtcggac cgggtgtcgg cccgcatggg cggcaactcg
      601 gtcatggtga cgcccgacac gatcgagtac atggacgtct ccccgaagca gctggtgggg
      661 atctccacct ccctcatccc gttcctggag cacgacgacg ccaaccgggc cctgatgggc
      721 gccaacatgc agcgccaggc ggtgcccctg ttacgtccca ccgctcccac ggtgggaacc
      781 ggcatggagt tcgtggcggc caaggattcc ggcgccgtgg tcgtggccaa gcgggggggc
      841 gtggtggagt cggtctcggc cgagcggatc ctggtgcgtg ccgatcccaa agagacacgc
      901 tccgacgggc aggacaacgg ctcgggtgtg gacatctaca ccctcttgaa gttccgccgc
      961 agcaaccaga atacctgcat caaccagaag cccatcgtgg ccaagggtga tcgggtgatg
     1021 aagggccagg tgattgcgga cggacccgcc acccagcagg gcgaactggc cctggggcag
     1081 aacgtcctgg tggccttcat gccctggggc gggtacaact tcgaggacgc catcctcatc
     1141 agcgagcgcc tggtgaagga cgaccgctac acctccatcc acatcgagga cttcgagatc
     1201 gaggcccggg acacaaagct gggccgcgag gagatcaccc gggacatccc caacgtgggg
     1261 gaagaagcgc tgaaggacct ggacgaaagc gggatcatcc gcatcggggc cgaggtcaag
     1321 cccggtgaca tcctggtggg caaggtgacc cccaagggcg agtcggtcct caccccggaa
     1381 gagcgtctct tgcgcgccat cttcggcgag aaggccgagg acgttcgaga cgcctccctg
     1441 tacgtccccc cggggatcag cgggaccgtg gtggacgtga agatcctctc ccgcaagggg
     1501 gcggagaagg atgagcgggc caagtccatc gaggacgaag aggtttcgcg cctgcgcaag
     1561 gacttcgagg acgagatcca gatcatccag tccgagcggg acgtcaagat ccggaaacag
     1621 ctggaagacc ggatcgtggg caaggacgtc cgggggcgcg gcagccggaa ggtgatcatt
     1681 ccgaagcggc acaagctcac cgccgaggtc ctggaggggc tgaccaccga ggacatgcag
     1741 gacgtgggtc tgcagctgga cgacgagacg gccgccgagg tgacccagtt gatcgagggg
     1801 gcggagaacc aagtccacgt cctggagagc ctgctggaag agcgcatcgg ccgtgtgaag
     1861 cggggggacg aactcgcccc cggcgtcttc aagctggtga aggtcttcgt ggccatgaag
     1921 cgcaagctgt cggtgggcga taagatcgcc ggccggcacg gcaacaaggg cgtcatcgcc
     1981 aagatcctac ccgaagagga catgccctac ctgcccgacg ggacgccggt ggaggtggtg
     2041 ctgaatcccc tgggcgtgcc gtcccgtatg aacgtgggcc aggttctgga gacccatctc
     2101 ggctgggcgg ccgccgccct gggactgcgg gcggcctcgc cggttttcga cggcgcctcc
     2161 gagaaggaca tcaagctgat gctgaagcgg ggcaacctcc cgccctcggg gaaggtgctg
     2221 ctctgcgatg gccgcagcgg gaagcccttc caccaggagg tcgccgtggg gtacatctac
     2281 ctgatgaagc tggcccacct ggtggacgac aagatccacg cccgctccat cggaccctat
     2341 tccctggtca cccagcagcc cctgggcggc aaggcccagt tcggcggcca gcgattcggc
     2401 gaaatggagg tctgggcctt ggaggcctac ggagcggccc acacgctcca ggaagtcctc
     2461 acggttaaat ccgacgacgt gatcgggcgc accaagatgt atgagtccat cgtcaaaggc
     2521 gagaacgcgc tggagccggg tctgcccgag tccttcaacg tgctggtgaa ggaactccag
     2581 agcctggccc tggacgtcga gttggtcaag gaatagtacg gagtcgcgcc ggcgcgatta
     2641 ctaaaggagc acccagtgga gaagttattc gggatcatcc aagagaatcc tgttgacacc
     2701 accagcttac gggccatccg catctg
//
LOCUS       DBNGZC010001083         3178 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1165019, whole genome shotgun sequence.
ACCESSION   DBNGZC010001083 DBNGZC010000000
VERSION     DBNGZC010001083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1165019"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..1657)
                     /gene="guaA"
                     /locus_tag="AC16G0_18670"
     CDS             complement(125..1657)
                     /gene="guaA"
                     /locus_tag="AC16G0_18670"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR5101685.1"
                     /translation="MDKVLILDFGGQYTQLIARRVRELGVYSEIQPFRLPLHEIRAFQ
                     PTALILSGSPASIYDPTAPRPDPGILDLGVPVLGICYGLCILADLSGGEVSRAARREY
                     GPADLLVDRGEDLFAGLPGSLKVWMSHGDKVDKLPAAFSVLAHSGNSPYAAFRHRDRP
                     FFGVQFHPEVVHTPQGKDILANFLFRIARCEPSWDMKSFVASSVERIREQVGDGQVVC
                     GLSGGVDSAVAALLVHRAIGDRLTCIFVDNGLLRLNEAWQVVETFGTHLGLNLIAVDA
                     SDRFLAKLAGVEDPERKRKIIGAEFIAVFEEQAKRLGRFDFLAQGTLYPDVIESVSVK
                     GPSVTIKTHHNVGGLPEKMGFKLVEPLRELFKDEVRAVGRELGLPDVILWRQPFPGPG
                     LAVRCIGDITRERLDLLRKADVIVEEEIRKAGLYQEIWQAFAVLLPIRTVGVMGDERT
                     YEHVVALRAVHSQDGMTADWARLPYDVLATISSRIANEVKGVNRVVYDVSSKPPSTIE
                     WE"
     gene            complement(1721..2074)
                     /locus_tag="AC16G0_18675"
     CDS             complement(1721..2074)
                     /locus_tag="AC16G0_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR5101686.1"
                     /translation="MFRFEQLKVWHKAVELYDVADDLADRFPAKVKFGLADQFRRAAL
                     SISSNIAEGSGRETAKEFRHFLSIAKGSAYEMVSIAFVCRRRGLITDQQHADLYCRAE
                     EVSKMLTGLKRSPVS"
     gene            complement(2161..>3178)
                     /gene="guaB"
                     /locus_tag="AC16G0_18680"
     CDS             complement(2161..>3178)
                     /gene="guaB"
                     /locus_tag="AC16G0_18680"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR5101687.1"
                     /translation="VMTKENLVTAPIGTSLEEAKEILHRHRIEKLPVVDEDFNLKGLI
                     TIKDIEKRIKYPDACKDVLGRLRVGAAVGVGPEALERVPQLIRAGADVLVVDTAHGHS
                     AAVIDMVKTIKRRYPGAEVVAGNIVTAEAAEDLIQAGADAIKVGIGPGSICTTRVVAG
                     AGVPQISAIRQCAAVADRSDVPLISDGGIKYSGDITKAIAAGAHSVMLGGLFAGTEES
                     PGETVLFQGRTYKVYRGMGSLGAMQSRGARERYYQDLEADMEKLVPEGIEGRVPYKGT
                     LSALVYQLVGGLRAGMGYCGCRTIEDLRTKAKFVRLTFAGLRESHVHDVIVTKEAPNY
                     RLES"
BASE COUNT          539 a         1118 c          958 g          563 t
ORIGIN      
        1 acggcttacc cgtgcagacg acgggatcta cagtaatgcg ctcgaacttc attggctcct
       61 caccccactc tctcccatgg gtttcgcgaa gcaagcgtcc tggaaaggcc tcgcggtcgg
      121 cgtcctattc ccactcgatg gtgctggggg gcttgctgct cacgtcgtag acgacgcggt
      181 tgacgccctt gacctcgttg gcgatccggc tgctgatggt cgccagcaca tcgtagggca
      241 ggcgggccca gtccgcggtc atgccgtcct ggctgtggac ggcgcggagc gccacgacgt
      301 gctcgtaggt cctctcgtct cccatgacgc ccacggtccg gatgggcaac aggacggcga
      361 aagcctgcca gatctcctgg tacaggcccg ccttccggat ctcctcctcc acgatcacgt
      421 ccgccttgcg gaggagatcg agccgctccc gcgtgatatc gccgatgcag cgcacagcca
      481 gccccggccc ggggaacggc tggcgccaga gaatcacgtc cggcaatccc agctcgcgtc
      541 ccactgctcg cacctcgtcc ttgaacagct cccgcagggg ctcgaccagc ttgaagccca
      601 tcttctcggg gagaccgccc acgttatggt gcgtcttgat cgtgaccgag ggtcctttca
      661 ccgagacgct ctcgatcacg tccgggtaga gcgtaccctg ggccagaaag tcgaacctcc
      721 cgagtcgctt cgcctgctcc tcgaagacag caatgaactc ggccccgatg atctttcgct
      781 tccgctcggg gtcttccacc cccgccagct ttgccaggaa gcggtcgctg gcgtccacgg
      841 caatcaggtt cagccccagg tgggtcccga aggtctccac cacctgccag gcctcgttca
      901 accggagcaa cccgttatcc acgaagatgc aggtcaggcg atcgccgatg gcccgatgga
      961 ccagcagggc cgccaccgct gaatccacac cgccgctcaa cccgcagacc acctggccgt
     1021 ccccaacctg ttcccggatt cgctccacgc tgctggccac gaacgacttc atgtcccatg
     1081 acggctcgca cctggcgatc cggaacagga agttcgccag gatgtccttc ccctggggcg
     1141 tgtgcacgac ctcgggatgg aactgcaccc cgaagaatgg ccgatcccgg tggcggaaag
     1201 cggcgtaggg agagttgccc gaatgggcca aaacggagaa tgcggccggc agcttgtcca
     1261 ccttgtcccc gtggctcatc cagaccttga ggcttccggg aaggccggcg aacagatcct
     1321 ccccccggtc caccaggagg tcagcggggc cgtactcccg ccgggccgcc cgtgacacct
     1381 cgcctccgga gaggtcggcc aggatgcaca gcccgtagca gatcccaagc accggcaccc
     1441 cgaggtccag gatccccgga tcgggacgcg gggccgtggg gtcgtaaatg ctggccgggc
     1501 tgcccgagag gatcaaggcg gtgggttgga aggcccggat ctcgtggagc ggcagacgga
     1561 agggctggat ctcggagtag acgccgagct cccgcacccg tcgggcgatc aactgcgtgt
     1621 actgaccgcc gaagtcgagg atcagcactt tatccatgct agcgcctagc gccgaggccc
     1681 gagcgccaag aatatagctg ggctgcagag gtcctcggag tcaagaaaca ggtgagcgct
     1741 taagccctgt gagcatcttg gagacttctt ccgcccggca gtaaaggtcg gcatgttgct
     1801 ggtcggtgat caggccgcgc cgccggcaga caaatgcaat actcaccatc tcataggccg
     1861 accctttagc tatgctgaga aagtgccgga actcctttgc ggtttctcgg cctgaacctt
     1921 cggcgatgtt cgaggatatc gagagcgctg cgcggcgaaa ctgatctgcc aacccaaact
     1981 tcacctttgc cgggaaccga tcggccaagt catccgccac atcgtaaagc tcgaccgcct
     2041 tgtgccaaac cttgagctgc tcgaaccgaa acatagcagt ctcctccggc cgtcgtctgg
     2101 tgatccgctt ctcttggcgc tcggcgctcg gcgctcggcg ctggtcccgg ctctcggctc
     2161 tcagctctcc agccggtagt tcggcgcctc cttcgtgacg atgacgtcgt ggacatggct
     2221 ctcgcgcaaa ccggcgaagg tcaggcggac gaacttggcc ttggtccgca gatcctcgat
     2281 ggtccggcag ccgcagtacc ccattccggc ccgcagccca cccaccaact ggtacaccag
     2341 ggccgagagg gttcccttgt acggaacacg cccctcgatc ccctcgggca ccagcttctc
     2401 catgtccgcc tcgagatcct gatagtaccg ctctcgcgcc ccccgggact gcatggcccc
     2461 gagcgagccc ataccgcggt agaccttgta ggtccgcccc tggaacagca ccgtctcgcc
     2521 ggggctctcc tcggttcccg caaacaggcc gcccagcatc accgagtgcg ccccggcggc
     2581 gatggccttg gtgatatccc cggagtactt gatgcccccg tccgagatca ggggcacgtc
     2641 cgaccggtcc gccacggcag cgcactggcg gatggcgctg atctgcggca cgccggcccc
     2701 cgccaccacg cgggtggtgc agatggagcc gggtccgatt cccaccttga tggcgtcggc
     2761 cccggcctgg atgagatcct cggcagcctc ggcggtgacg atgttgcccg ccaccacctc
     2821 ggccccgggg tagcgccgct tgatggtctt gaccatgtcg atgacggcgg cagagtgtcc
     2881 gtgcgccgta tccaccacca ggacgtccgc gcccgcccgg atgagctgcg ggacccgctc
     2941 cagggcctcg ggccccaccc cgaccgccgc gcccacccgg agtcgaccca ggacatcctt
     3001 gcaggcgtcg ggatatttta tacgcttctc gatgtccttg atggtgatca gccccttcag
     3061 gttgaagtcc tcatccacga ccggcagctt ctcgatccgg tggcggtgga ggatctcttt
     3121 tgcctcctcc aggctcgtgc cgatcggcgc cgtcaccagg ttctccttgg tcatcacg
//
LOCUS       DBNGZC010001084         1778 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1465377, whole genome shotgun sequence.
ACCESSION   DBNGZC010001084 DBNGZC010000000
VERSION     DBNGZC010001084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1465377"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="AC16G0_18685"
     CDS             <1..261
                     /locus_tag="AC16G0_18685"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR5101688.1"
                     /translation="HRVMASTLAAVVALALFPLALGHTLYNAALRRLHASIPNLIATQ
                     EVTGGVLLAWLLLHEVPPWNALVGAALTLVGVGLVLCCPSVN"
     gene            457..642
                     /locus_tag="AC16G0_18690"
     CDS             457..642
                     /locus_tag="AC16G0_18690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101689.1"
                     /translation="MPNLYTIYCPAVPEHRVSWVPIPEPYQTLPLMAPGKPGKNPELV
                     RVLQRCPNCVVRLLMGR"
     gene            715..1218
                     /locus_tag="AC16G0_18695"
     CDS             715..1218
                     /locus_tag="AC16G0_18695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR5101690.1"
                     /translation="MRHGTVVVGLLLVVTLSGCVSRHNIKDFQPGNTDEAQVMSALLK
                     IPTGINVKSVDIVMSPYADDVYIGNFHKYLGVASPSAPRTITKEELRSAYTQLFKSVK
                     DLSLDVVNFQLTVTGDRAAAEARMELLVRIEAARKESRLETIRNDILWRLKRTPAGWK
                     IQEEIYE"
     gene            complement(1290..>1778)
                     /locus_tag="AC16G0_18700"
     CDS             complement(1290..>1778)
                     /locus_tag="AC16G0_18700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR5101691.1"
                     /translation="PYRILGADTGPVQVGEARFEFLHPLTSRGSVLEEGKGTRSRSNA
                     TSLVMRVSLGNQALLLTGDLERDGESVLLEESRPIHAEVLTVPHHGSRMASTAGFIQT
                     VRPDVALVSVGYRNRFRHPHPEVVERYLALGVRLLRTDLNGAINVEMTGENSRAWGRR
                     KE"
BASE COUNT          329 a          593 c          520 g          336 t
ORIGIN      
        1 catcgcgtga tggcgagcac cctggccgcc gtcgtcgccc tggccctgtt cccgctggcc
       61 ctcggccaca cgctgtacaa cgcggccctg cggcggcttc acgccagcat cccgaatctc
      121 atcgccacgc aggaagtcac gggcggagtc ttgctcgcct ggctgctcct ccacgaggtg
      181 cccccctgga acgccctggt cggcgccgcc ctcaccctgg tcggcgtcgg tctggtccta
      241 tgctgcccct cggtgaactg acgatcagac aagccggcac ggtggagatt ccgtgcggcc
      301 actcgcgcga tcactcgctg ctcgactggt tgggcgggcg aatcgcccag gggtcagatt
      361 gtctgcctgc ccgagcgatc aaagaccatg ctccctcttg ccagttcggc tgcactcgcg
      421 tataagaata ggcaagacat gaatctggag gctgcgatgc cgaatctcta cacgatctac
      481 tgtccggcgg tgccggaaca tcgtgtgagc tgggtcccca tcccggaacc gtatcagacg
      541 cttcccctca tggccccggg caagccggga aagaacccgg aactcgtgcg ggtgctccag
      601 cgctgtccga actgtgtggt gcgcctgttg atgggacgct gaaatcgacg ggatggcacc
      661 agcggatctc gggtaatccg gcgaactgag ccgcggcaca aagggaggta tctcgtgagg
      721 catgggacgg tggtggtggg actcttgctc gttgtgacgc tctccggctg tgtttctcgg
      781 cacaacatca aggattttca acccgggaat accgacgaag ctcaggtgat gtccgcgctt
      841 ctaaaaatcc ctaccgggat caacgtcaag tccgtggata tcgtcatgtc accctatgcc
      901 gatgacgtgt acatcggaaa cttccataag tatctgggtg tggcgtcgcc cagcgccccg
      961 cgaaccatca ccaaggagga gctccgttcc gcgtataccc agctcttcaa gtcggtcaag
     1021 gacctctccc tggatgtggt gaactttcaa ctcacggtga ccggcgatcg agcagcggcc
     1081 gaggcccgga tggaactctt ggtcaggata gaggccgcca ggaaggagtc caggctggaa
     1141 accattcgca atgacatctt gtggcggctg aaacgcacac ccgcaggatg gaaaatccag
     1201 gaggagatct acgagtaggc cgaccccctc gcaccattcg actgtcggcc gatcctgaaa
     1261 acccgtcggc cgaagggccg tgctgccggc tactccttcc gcctccccca cgctcggctg
     1321 ttttcaccgg tcatttccac gttgatggcc ccattcaggt ctgtccgtaa cagtctcacc
     1381 cccagggcca ggtaacgctc caccacctcg gggtgcgggt ggcggaaacg attccgatat
     1441 cccaccgaca ccagtgccac atccggccgg accgtctgga taaagcccgc ggtgctcgcc
     1501 atccgggacc cgtgatgtgg gaccgtcaac acctcggcat ggatcggcct tgactcttcc
     1561 aggaggaccg attctccatc ccgctcgaga tctcccgtga gcaggagtgc ctgattcccc
     1621 aggcttaccc gcatcaccag ggaggttgca ttggatcgcg accgcgtccc cttgccctct
     1681 tcgagcacgc tccccctgct ggtgagagga tgcaggaact cgaaccgggc ctcccccacc
     1741 tggaccggac ccgtatccgc ccccaggatc cgatacgg
//
LOCUS       DBNGZC010001085         1663 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_583246, whole genome shotgun sequence.
ACCESSION   DBNGZC010001085 DBNGZC010000000
VERSION     DBNGZC010001085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_583246"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="AC16G0_18705"
     CDS             complement(<1..662)
                     /locus_tag="AC16G0_18705"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5101692.1"
                     /translation="MATRLTVITENTVPGKADGLYAEHGFSLFLDRPEVKLLFDTGPA
                     GIATLHNAPRLGLDVRAADGIVLSHGHQDHTGGLAGVLHAMDKRVRVYAHPGIFADRY
                     GKRGDRVRYSGLLFKREALEGLGADFDLSSDFREIAPGVHLSGEIPRRRAFETGDAEL
                     FVKRGGELVKDPLPDDQSLGVETAEGLALILGCCHAGLINTLDHFQARLPGKPIHTVI
                     GGT"
     gene            complement(728..922)
                     /locus_tag="AC16G0_18710"
     CDS             complement(728..922)
                     /locus_tag="AC16G0_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgaP family membrane protein"
                     /protein_id="HIR5101693.1"
                     /translation="MTLERWIRLIAGSFILLSLVLSQLQSPYWLLFTAFVGVNLFQSA
                     LTRWCLMEDILRKLGVREST"
     gene            complement(919..1392)
                     /locus_tag="AC16G0_18715"
     CDS             complement(919..1392)
                     /locus_tag="AC16G0_18715"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR5101694.1"
                     /translation="MSQSAEETSRTMLRREEGYRFRVRFDHEGMPDLLTDEAPPIGEG
                     KGPTPSRLLAPAGANCLAASLLFCLEKARLSLEGLEAEAVTEFTRNEAGRLRIGGIRV
                     RLSPQWTGETAAKAQRCLGIFENFCVVTESVRHGVPVTVEVDGMPSVSGGAGGKS"
BASE COUNT          291 a          578 c          501 g          293 t
ORIGIN      
        1 gtgcctccga tcaccgtgtg gatcggcttg cccggaagcc ttgcctggaa atgatcgagc
       61 gtgttgatga gacccgcatg acagcatccg aggatcaagg ccagcccctc cgcggtttcc
      121 acccccagtg actgatcgtc tggcaggggg tccttgacca attcgccgcc tcgcttcacg
      181 aacagctcgg catcccccgt ctcgaaggcg cgccgacgcg ggatttcacc gctcagatgc
      241 acgcccggtg cgatctctcg aaaatccgag gaaaggtcga agtcggcgcc cagtccttcc
      301 agggcctccc gcttgaagag gagcccgctg tagcgcacgc ggtcgccccg cttgccgtag
      361 cggtccgcga aaatgccggg atgggcgtac acgcggaccc gcttgtccat ggcgtgcagg
      421 acaccggcaa ggccgcccgt gtgatcttgg tggccgtggc tcagcacgat gccgtcggcg
      481 gcccggacat ccagtcccag gcgcggcgcg ttgtggagcg tggcgatgcc cgccggaccc
      541 gtatcgaaca ggagcttgac ctccggccgg tccaggaaca aggaaaaacc gtgctccgcg
      601 tacaggccgt ccgccttccc cggcaccgtg ttctcggtga tcaccgtgag tctggtcgcc
      661 acgctctcat cctttctcgc cccttggcgt tcttggcgcc ttggcgattg gaattcccgg
      721 ccgaccctta ggtggattcc cggacgccca gcttccgcag gatatcctcc atgaggcacc
      781 agcgcgtcag ggccgactgg aacagattga cgccgacaaa agccgtgaac agcaaccagt
      841 aggggctctg tagctggctc aggaccaggc tcaggaggat gaagctcccg gcgatgagac
      901 ggatccagcg ctcgagtgtc atgactttcc tcccgctccc ccggaaaccg agggcatacc
      961 atccacctcc accgtcaccg gcaccccgtg ccgcaccgac tcggtcacca cgcagaagtt
     1021 ctcgaagatc ccgaggcaac gctgggcctt ggccgccgtc tccccggtcc attgcggtga
     1081 gaggcgcaca cggatgcccc cgatccgcag ccggcccgcc tcgttccgcg tgaactctgt
     1141 gacggcctcg gcctccaggc cctccaggct gaggcgcgcc ttttccaggc agaagagcag
     1201 gctggcggcc agacagttgg cccccgcggg ggccagcagg cgggatggag tcggcccctt
     1261 cccctcgccg atcgggggag cctcgtcggt gagcaggtca ggcatcccct cgtgatcgaa
     1321 gcggacccgg aagcggtatc cctcctcccg tcgcaacatg gtccgactgg tctcctcggc
     1381 tgactggctc atggcttctc cttgctctga tccgactcgt tctcagtgct cgacgagcac
     1441 ccctgggtcc ctcaggtggg tacaccagct ttgcgcaaga cgaactgcag gacgagccag
     1501 acccccacca cagccgcgat tgttccgatt tgtccgagcc aatccattgt accctccttg
     1561 tgaggcacag ggcaaagcgt tgacccggac tattcgcgaa cgagttcgcg aatagtcgcg
     1621 ggaatgacca aatcccaatg cccaatggcc aattgcggat tgc
//
LOCUS       DBNGZC010001086         3400 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_96386, whole genome shotgun sequence.
ACCESSION   DBNGZC010001086 DBNGZC010000000
VERSION     DBNGZC010001086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_96386"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="AC16G0_18720"
     CDS             <1..222
                     /locus_tag="AC16G0_18720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101695.1"
                     /translation="GLGLGDRQGKGRILLGVKPDDSAGVAFYDKDGTLRAVLGLTAIP
                     VAPTGLVPPEPASAMALFDRDGKVVHQVP"
     gene            367..507
                     /locus_tag="AC16G0_18725"
     CDS             367..507
                     /locus_tag="AC16G0_18725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101696.1"
                     /translation="MRSVRWTIPGFTLLLLGGGNARAAVAVQEGTQIIQDMAILRRDN
                     IR"
     gene            516..1670
                     /locus_tag="AC16G0_18730"
     CDS             516..1670
                     /locus_tag="AC16G0_18730"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018132288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmpA family aminopeptidase"
                     /protein_id="HIR5101697.1"
                     /translation="MPSHETDGGRRKGVSRPRARDLGLVAAELPTGMENAITDVAGVR
                     VGHTTLISGDGPLRPGEGPVRTGVTVVLPQADNLFRLKVRAAVHTINGFGKTCGFEEV
                     RELGMIEAPIALTATLNVGLVLDALVQYSVRQSPEIGIRTSNVNVVVGECNDGFLNDM
                     QGRHVRAEHVWAAIDAAASGPVPEGAIGAGTGTSCFGWKGGIGTASRMVPPEAGGFTV
                     GALAQTNFGRPRDLTICGVPIGQHLEPPGSSGPPAPERGSVMIVLATDAPLSARQLWR
                     LSVRAAAGLVRVGSHHGHGSGDFVIAFSTANKIEHTPVSITTTQTVLADEPKAMAWLF
                     PAVVESVEEAVLNSLAQAKTVVGRDGNTRHALPLGVVAEMVRRCRPAAAE"
     gene            1984..3390
                     /locus_tag="AC16G0_18735"
     CDS             1984..3390
                     /locus_tag="AC16G0_18735"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5101698.1"
                     /translation="MRMSALVGIGVLAFLAVGLVAPTTTVSAGETSLAYFQQAKINWR
                     QFEGQKLTIGLNKHPYTESLLPLIPEFEALTGIKVEYLILPENEYGAKLTADLSQQRG
                     EFTVIMAGAMRNWAYVTPGWMVPLDQFLNDPKLTDRSWYKLEDFFHSLIAANRWNGKA
                     AGGTGEGPLWSIPVMSESYVLAYRKDVFDRYKIKVPRTYEEMAEAARLVKKNAGIDGL
                     VSRGTPNFPTIATGYLSGVKSYTDGKWAELDEKMNAQFQDPRVVKFSQMWTDMLRESG
                     PANWASMTWYDGMETFAAGQAGMYPDCDFFAATYEDPKKSKVAGKVGYALLPPGPAGT
                     TYSGLFTWALGMSSATKNKGAAWLFIQWATSPRTLLNATVEFQNYSPTRLSVMNDPRT
                     QKVMGGWGNGTYVKTVQENLKTARIAWVPEPERPRLGDVWARALHEIYFKRLSAEDAM
                     KRATDEVNKIFTEIGLRK"
BASE COUNT          632 a         1002 c         1210 g          556 t
ORIGIN      
        1 gggttgggac tgggcgaccg acaggggaag ggtcgcattc tcctcggggt gaagcccgat
       61 gattcggccg gtgtggcctt ctatgacaag gatggcactc ttcgggccgt cctgggactg
      121 accgccattc cggtggcgcc aaccgggctc gttccgcccg aacctgcctc ggcgatggcg
      181 ctgttcgaca gggacgggaa ggtggtgcac caggtcccgt aagtggaacc tttgctttgt
      241 gcactcgtca gcctcgaggc ggtggcgaaa cgtaaaccgg gtggaaggta tcatcgggct
      301 gattcagccg aagcccgtgg agggtcggtg gtaggaggcg gggaatattt cccggaggag
      361 gcatccatgc ggagcgtcag gtggactatt ccgggattca cgctgctgct gctcggcgga
      421 ggaaacgcgc gggcggcagt ggcggtgcag gaggggaccc aaatcatcca ggacatggca
      481 atcctccgtc gagataatat ccgatgactg cggcgatgcc atcccatgag accgacggtg
      541 gccggcggaa gggagtgagc cggccgcggg cgcgtgacct ggggctggtg gcagcggaat
      601 tgccgacggg aatggaaaac gccatcaccg acgtggccgg agtccgcgtg ggacatacca
      661 cgctcatctc gggcgacgga ccgctccgcc caggtgaggg acccgtgcgg accggggtga
      721 cggtcgtcct gccgcaagcc gacaacctgt tccgcctgaa ggtgcgggcg gcggtgcata
      781 cgatcaacgg gtttgggaag acgtgcgggt tcgaagaggt tcgcgagctg ggcatgatcg
      841 aggcgcccat cgccttgacc gccacgctga atgtgggtct ggtgctggat gccctggtgc
      901 agtattcggt ccgccagagc ccggagatcg ggatccggac cagcaatgtc aacgtggtcg
      961 tgggcgagtg taacgatggg ttcctgaacg atatgcaggg gcggcacgtc cgtgccgagc
     1021 acgtttgggc ggccatagac gccgctgcct cggggccggt tccggaaggg gccatcggcg
     1081 caggcaccgg gaccagctgt ttcggctgga aggggggaat cggaacggcc agccggatgg
     1141 tcccaccgga ggcgggaggc ttcacggtcg gcgccctggc gcagacgaat tttggccgac
     1201 cgcgcgacct caccatctgc ggggtcccca tcggccaaca tctggaaccc cccggatcct
     1261 ccggtccgcc cgcacctgag aggggatcgg ttatgatcgt gctggccacc gatgcgcccc
     1321 tgtctgcccg ccagctctgg cgcctgagcg tccgggcagc cgctggactc gtgcgggtgg
     1381 ggagtcatca cggccacggg agcggcgact tcgtgatcgc cttcagcacc gcgaacaaga
     1441 tcgagcacac gccggtctcc atcacgacga cccagacggt gttggccgat gagccgaagg
     1501 cgatggcgtg gctctttccg gcggttgtcg aaagcgtcga ggaggcggtg ctcaactctc
     1561 tggcgcaggc gaagacggtc gtgggtcggg acgggaacac acggcatgcg ctgccgctcg
     1621 gggtggtggc ggagatggtc cggcggtgca gaccggcggc agccgagtga ttcctcgagg
     1681 cgcgagagat gccggggcga cgccaaagac cttgaccggc cggggatggt ggatagacgc
     1741 gtgtccgctt caggcaaggg agtgatgctc ccggagcgac cccacgttcc gacggcagag
     1801 gagacgtccc gtgatggccg tccggggtgg cacaacccgg ggagcctttt tcgtttctga
     1861 atgcgcactg cacgcccgcg cctgaggatg catgcgtacc aggaggaggt gagaactgga
     1921 gacgttttgg gcgacaggct ggctcaaatc ctcggaggcg agaaggagga cgcacagatg
     1981 cggatgcgaa tgagtgcgct cgtcgggatc ggggtgctgg cgttcctggc ggtcggactg
     2041 gttgccccga ccacgacggt cagtgcgggc gagacttcgc tggcctactt tcagcaagcc
     2101 aagatcaact ggcggcagtt cgaagggcag aagctgacca tcggactcaa caaacatccc
     2161 tacacagagt cgctgctgcc gctcatcccg gagttcgagg cgttgaccgg gatcaaggtg
     2221 gaatacctca tcctgccgga gaacgagtac ggcgccaagc tcaccgccga cctgtcccag
     2281 cagcgcgggg agttcaccgt catcatggcg ggggccatgc ggaactgggc ctacgtgacc
     2341 cccggctgga tggtgccgct ggaccagttc ctgaatgacc cgaagctcac ggataggtcg
     2401 tggtacaagc tggaggactt cttccacagt ctcatcgccg ccaatcgctg gaacggcaag
     2461 gccgccggcg ggacggggga ggggccgctc tggtccatcc cggtcatgtc agagagctac
     2521 gtcttggcct accggaaaga tgtcttcgac cggtacaaga tcaaggttcc caggacctac
     2581 gaggagatgg ccgaggccgc ccgtctggtc aagaagaacg ccgggatcga cggcctggtg
     2641 tcccgcggga cgccgaactt ccccaccatc gccaccgggt acctcagtgg cgtgaagtcg
     2701 tacaccgacg gcaagtgggc cgagctggac gagaagatga acgcccagtt ccaggatccg
     2761 cgcgtggtca agttcagtca gatgtggacc gacatgcttc gcgagagcgg cccggccaac
     2821 tgggccagca tgacctggta cgatggcatg gagaccttcg ctgccggcca ggccgggatg
     2881 tatccggact gcgatttctt cgccgccacc tacgaggatc cgaagaagtc caaggtggcg
     2941 gggaaggtcg ggtatgctct cctgccgcct ggtcccgccg gtacgacgta ctccgggttg
     3001 ttcacctggg ccctcggcat gagcagcgcc acaaagaaca agggggcggc ctggctgttc
     3061 atccagtggg ccacctcgcc gcgcaccctc ctgaacgcga cggtggagtt tcagaactat
     3121 tctccgaccc ggctgtcggt catgaatgat ccccggaccc agaaggtgat gggcggctgg
     3181 gggaacggga cctacgtgaa gacggtgcag gaaaacctca agaccgcgag aatagcctgg
     3241 gtacccgagc cggagcgtcc caggttggga gacgtctggg cccgggccct gcacgagatc
     3301 tacttcaagc ggctgtccgc ggaagatgcc atgaagcgcg ccacagacga ggtgaacaag
     3361 atcttcaccg agatcggact ccgcaagtaa cggcgctcgt
//
LOCUS       DBNGZC010001087         3519 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1165260, whole genome shotgun sequence.
ACCESSION   DBNGZC010001087 DBNGZC010000000
VERSION     DBNGZC010001087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1165260"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1251)
                     /locus_tag="AC16G0_18740"
     CDS             complement(<1..1251)
                     /locus_tag="AC16G0_18740"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR5101699.1"
                     /translation="MTRTEQLRKDVFRILLVSTVDKAVLGQDFYFRFPHLSLPTLASY
                     TPEGAEVEIVDEKFRPLPDPRGYDLVGITAMTPLAPRAYRIADTCRRQGVPVVLGGYH
                     PTVLPDEALGHADSVCIGEAERIWPAIVADAMAGRLQERYRSETFPCLEDLPKPRRDL
                     LKIPRSRRFEHINVYFLQTTRGCPYRCSFCAVTSVLGGKLRHRPVAEVEAEIESLGIR
                     RLDRQAKRDRFHNIIFFTDDNIVGHRSYSRELLRMVSSFNLKWVGQASTNVADSEEIL
                     TLLRDSGCMGLAVGFETLSQKNIRDVGKGVNRTQEYLDRIRKIHSYGIGLAGNFIFGF
                     DQDDETTFAEVVEFVDAARLDGFYYSLLTPYPGTPFFDQMETEGRILERDWSLYDTDH
                     VVYAPRLMDAETLMKGYRWAWRRSL"
     gene            complement(1274..2275)
                     /locus_tag="AC16G0_18745"
     CDS             complement(1274..2275)
                     /locus_tag="AC16G0_18745"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5101700.1"
                     /translation="MAWLTLAGVGCSTVVKNVDQAVYSKPKYGTDRFVLVKGYHIHYV
                     EGGQGPPILLIPGAFTTYRTWNRVLPALSLHHRVLAVDYVGVGDSDKPEKGFQYTIQE
                     QADVMAEMVQALGLSRVNVVGASYGGAVALNLAARYPNLVSKVVCIEGGALITPEALN
                     YSRLGDLIEWPILGSIIWGFMKSGLFDGITARSVMGTAWEELTPEEKKDVTGIFAANI
                     MTASRSSWIKIYRAITSRIDFIQTVEQTRVPVLYLYGEDSKYRAVAEMNAKRFEEHNP
                     NIEVISFQGGIHDLHLQYPRDVSRIISRFLGGAPEVIAGGGAVGGIGQPADVGGAIP"
     gene            complement(2340..3002)
                     /locus_tag="AC16G0_18750"
     CDS             complement(2340..3002)
                     /locus_tag="AC16G0_18750"
                     /inference="COORDINATES: protein motif:HMM:NF026968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR5101701.1"
                     /translation="MKVDRGEEFLSGYTRVHARHPRPMVQECLPPGGEALGVGVLMNF
                     ASESKAAFAYRRLREYPITGGPSTLRESIWDDALVGIAERLLSLLGWVGVAMVEFKVD
                     PRDGRPKLLEVNPRFWGSLQHAIACGVDFPFLLYSLAMEGDIRRLDGYQVGRRSRSLL
                     HGDLLHFLKNPNRLHLKPSFYDISIPDDVLSLEDPFPTFGRIASIVAIVLDEELRAIA
                     GG"
     gene            3260..>3519
                     /locus_tag="AC16G0_18755"
     CDS             3260..>3519
                     /locus_tag="AC16G0_18755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101702.1"
                     /translation="MGKSTSYRPVASSRSKASRNPFGCLATEGWTSLRRQYRENAAGA
                     NRVLSPTAAKNPRRPSDRTAARVLFQKPSSISTRGAMPHRLGV"
BASE COUNT          678 a         1172 c         1034 g          635 t
ORIGIN      
        1 gaggctgcga cgccaggccc agcgataccc cttcatcagc gtctctgcat ccatcaggcg
       61 gggagcgtac acaacatgat cggtgtcata cagggaccag tccctctcga ggatgcggcc
      121 ctcggtctcc atctggtcga agaaaggcgt ccccgggtag ggggtcagga gagagtagta
      181 gaagccatcc agtcgagccg catccacaaa ctccaccacc tcagcgaagg tggtctcgtc
      241 atcctggtcg aacccgaaga tgaagttgcc ggcaagcccg atcccgtacg aatggatctt
      301 gcggatccgg tcgaggtact cctgggtccg gttcacaccc ttgcccacat cccggatgtt
      361 cttctgagag agcgtctcga atccgacggc cagccccata cagccggaat cccgaaggag
      421 cgtcaggatc tcctcgctat cggcgacgtt cgtggaagct tgccccaccc atttcagatt
      481 gaagctcgag accattcgca ggagttccct cgaatagctc cgatggccca caatgttgtc
      541 atccgtgaag aaaatgatgt tgtggaaccg gtcccgctta gcctggcggt ccaagcggcg
      601 gatccccagg gattcgatct cagcctcgac ctccgccacg ggccgatggc gaagcttccc
      661 tcccaggacg ctggtcacag cacagaacga acaccggtag gggcagcccc gtgtcgtctg
      721 caggaaatac acgttgatgt gctcgaaacg cctggaccgg ggaatcttga ggaggtcccg
      781 ccggggcttg ggcaggtcct ccaggcaggg gaaggtctcg gaccggtatc gttcctggag
      841 cctgcccgcc atggcatccg ccacgatagc gggccagatc ctttccgctt ccccgatgca
      901 cacgctgtcg gcatgcccga gcgcctcgtc cggcaggacg gtgggatgat acccacccag
      961 caccaccggc acgccctgcc ttcggcatgt gtctgcaatg cggtacgccc tgggcgcgag
     1021 cggagtcatg gccgtgatgc ccacgaggtc ataccccctt gggtccggga gcgggcggaa
     1081 cttctcatcc acgatctcca cctccgcccc ttccggggta taggaagcga gtgtgggcaa
     1141 gctcagatgg gggaaccgga aatagaaatc ctgccccagc acggccttgt ccaccgtgga
     1201 gacaagcagg attcggaata catcctttct gagctgttcg gtccgcgtca cgaagtgtcc
     1261 ccctgccgag ccattaggga atggcccctc cgacatcagc cggctgaccg attccgccaa
     1321 cggccccccc gcccgcaatg acttcgggtg cccctcccaa aaagcgcgag atgatccggc
     1381 tcacgtcccg ggggtactgg aggtgcaagt cgtggattcc cccctggaac gagatcacct
     1441 cgatgttcgg attgtgctcc tcgaatcttt tcgcgttcat ctccgcgacc gcccggtatt
     1501 ttgaatcctc cccatacagg tacaacactg gcacccgcgt ctgctccacc gtctggatga
     1561 agtcaatccg gctcgtgatc gcccgataga ttttgatcca cgacgatcgg gacgcggtca
     1621 tgatgttcgc cgcgaagatg ccggtgacat ccttcttttc ctcaggggtg agttcctccc
     1681 acgccgtccc catcaccgac cgggcggtga tcccgtcgaa gaggccggac ttcatgaagc
     1741 cccagatgat gctgcccagg atcggccact cgatgaggtc ccccagcctg ctgtaattca
     1801 acgcctccgg cgtgatcagg gctccgcctt cgatgcacac caccttgctg accaggttcg
     1861 ggtagcgggc ggccaggttc agggccacgg cccccccata ggaggcacca accacgttca
     1921 ccctggaaag ccccagcgcc tggaccatct ccgccatcac gtcggcctgc tcttggatag
     1981 tatactggaa ccccttctcc ggcttgtcgg agtctcccac gccgacgtaa tcgactgcca
     2041 ggaccctgtg gtgcaacgac agcgccggca gcacccggtt ccaggtccgg taggtcgtga
     2101 aggcccccgg gatgaggagg atcggcggcc cctggccgcc ctccacgtaa tgaatgtggt
     2161 agcccttgac caggacaaat ctatcggttc cgtacttcgg cttcgaatag accgcctggt
     2221 cgacgttctt cacgacggtg ctgcagccga cgcctgcgag cgtgagccag gccacggcga
     2281 tgatccgcca cgacctccgc catgcctgcc tgtacctgag ctctttagac agtaccatct
     2341 cagccacccg cgattgctct cagttcctcg tcgagcacga tggcgacgat ggaggcaatc
     2401 ctcccgaagg tcggaaaagg atcttccagc gagaggacgt cgtcgggtat ggagatgtcg
     2461 taaaacgacg gcttcaagtg gagccggttt ggattcttca gaaaatgcag caggtctccg
     2521 tgaagcaacg accggcttct ccgaccgacc tgatacccgt ccagtcggcg gatgtcccct
     2581 tccatcgcca gagagtacag gagaaagggg aaatcaaccc cacaggcaat cgcgtgttgg
     2641 agggatcccc agaatcgcgg attcacctcc agcagctttg gccgcccatc ccgggggtcc
     2701 accttgaatt ccaccatcgc gacccccacc cagccgagca gagacagaag cctctccgcg
     2761 attcccacca gggcgtcatc ccaaatgctt tctcgaagcg tgcttggacc gccggtgatt
     2821 ggatattcgc gcaagcgccg gtacgcgaag gctgccttgg attccgaggc gaagttcatc
     2881 aggacgccca cgcccaaggc ctccccgcct ggcgggaggc attcctgcac cattggtctg
     2941 ggatggcggg cgtgcactct ggtgtacccg gacaggaatt cctccccgcg gtccaccttc
     3001 acgatccccc gccccccccg acgaatggcg cggcttgatg aggaccgggt agggaagcgc
     3061 ggtggccagg gttgccgcct cctccggccc ctcaggcaag aacgtggcgg gcacatccat
     3121 gccgtgcgcc ctcgcgaacg aggccaggaa cgccttgtcg tcgatacgga aggtgagatc
     3181 gggatcggcg aagggaatcc ggaccagctt ttccaggcgc tcgcggttcc tcgtgacgag
     3241 caattgtgtg gagagctcca tggggaagag cacgtcatac cggcccgtgg ccagttcccg
     3301 ctccaaggcc tccaggaatc ccttcggctg tctggcgacg gagggatgga cgagcctgcg
     3361 acggcagtac cgtgagaacg cggctggagc gaaccgggtc ctctccccca cggcggcaaa
     3421 gaacccccgc cggcccagcg accgcacagc cgccagggtc ttgttccaga aaccgtccag
     3481 tatcagcact cgaggagcca tgccgcaccg cctgggcgt
//
LOCUS       DBNGZC010001088         3602 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2246092, whole genome shotgun sequence.
ACCESSION   DBNGZC010001088 DBNGZC010000000
VERSION     DBNGZC010001088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2246092"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1023)
                     /locus_tag="AC16G0_18760"
     CDS             complement(166..1023)
                     /locus_tag="AC16G0_18760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008382863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HIR5101703.1"
                     /translation="MTTGYRIVRKEVFSPVTYLWEVEAPALALAAQPGHFLMVRMHER
                     GERIPLTVADFDRAAGTVSVVIQAVGKSTREMMQLEAGDRLLDFIGPLGEASHIQHRS
                     KVILVGGGLGVAPIYPQLRAYKALGSTTLSIIGFRTQALIFWEEKFRDQSDELHIATD
                     DGSYGHKGFVSQVLQELLNRHKDTEEIVAIGQLPMMQACCEVSRPFGIRTMVSLNSIM
                     VDGTGMCGSCRVTVGGKMRFACVDGPDFDGHQVDFAELLRRQTRFRPEEQESLHHFED
                     ECKLMGQAR"
     gene            complement(1120..3141)
                     /locus_tag="AC16G0_18765"
     CDS             complement(1120..3141)
                     /locus_tag="AC16G0_18765"
                     /inference="COORDINATES: protein motif:HMM:NF014176.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR5101704.1"
                     /translation="MIGILVVGLPLLLLGAALEVSTSPLLCNQCHIMKPYYQAWKTSK
                     HNFVPCVDCHYPPGLRDTLVVKYQALSQVAKWATQTYSSKPFAEIEDASCLRSQCHAT
                     RLLEGKVLFKRGIIFDHRPHLEKPRRGRQLRCTSCHSQIVVGTHIEVTEATCFLCHFK
                     GKQTARDLQPIGGCPMCHQPPKGQIQLAAGVTFDHKEFVEARGARCQNCHLDAVQGDG
                     QAPRERCFTCHNQPEKLARYDDHEFLHDFHVTRHNIACLRCHTEIKHQIQTKKEPMSV
                     GCEGCHEAKHGGQRAMYLGIGGKGSPTIPSHMYTARVECVACHITPKVKDGHSRQFSG
                     QTFTASQAACVSCHGQQYAGMLDAWKRTLDTMLTSLKPKMEAVKKAVEGPAKDSKVLA
                     DARRLVEEAEFNFDFVENGKGVHNPFYAADLLQKVNASANRVMRLVGKSPIMIPQENL
                     VRGGYCATLCHSQAGVIFKPEVQFEKTTRVPHQNHFNQYGAVCTDCHSAEKHKAVTIT
                     REGCQACHHSAANDRCTTCHAAQAGLYGATLETVQSVKKEPNVMAGKVDCVGCHDLTR
                     KHSVATVTEKCIGCHDKGYRDMLAMWQGQVGGALEAARASLEKGEAALAAAKKDRREV
                     TAAAEFLAKARKDYAVVVKAKGIHNPDLAEAIFVESKKSAEQALGLLAK"
BASE COUNT          596 a         1166 c         1118 g          722 t
ORIGIN      
        1 gtgtagccta gcgccacctc gctgaagttc tgcccgcgag ccacaggatc ctgctcggta
       61 atgggggtcc ggccctgggg gatggtgtgg atcgtcttga ccgttgtcat cggaataatc
      121 tcccacgagc gggtcaaacg cagggggtga tccaccctgc ggccttcagc gcgcctgccc
      181 catcaatttg cactcgtctt cgaagtggtg gagggattcc tgctcctccg ggcggaagcg
      241 cgtctggcga cgcaggagct ctgcgaagtc cacctggtgc ccgtcaaagt ccgggccgtc
      301 cacgcaggcg aagcgcatct tcccccccac agtcacccgg caactcccgc acatgccggt
      361 cccgtccacc atgatggagt tgaggctcac catcgtccgg atgccgaagg gccggctcac
      421 ctcgcagcag gcctgcatca tggggagctg gccgatggcc acgatctctt ctgtatcctt
      481 gtgccgattc agcagctcct ggaggacctg gctgacgaag cccttatggc cgtaggagcc
      541 atcatccgtg gcgatgtgaa gctcgtcgct ctggtcccgg aacttctctt cccagaagat
      601 cagggcttgg gtccggaagc cgatgatcga gagggtggtg ctccccaggg ccttgtaggc
      661 ccgaagctgg gggtagatgg gggccacgcc caggccgccc ccaaccagga tgaccttgga
      721 ccggtgctgg atgtggctcg cctcccccaa tgggccgatg aagtcgagga gccggtcgcc
      781 cgcctccagt tgcatcatct cccgggtgga cttccccacg gcctggatga ccacgctgac
      841 ggtgcccgcg gcccgatcga agtcggccac ggtgagtggg atacgctccc cccgctcatg
      901 catccgcacc atgaggaagt ggccgggctg ggcggctagg gccagggcgg gggcctccac
      961 ctcccagagg taggtgaccg gggagaagac ctctttccgt acgatccgat agccggtggt
     1021 catgtctgca caccctgcct ctggtttatg gcgcatcgcc gaggcgccgg acctgccatg
     1081 gcccattgcc atgcgccgta cgccgtctgc cattcgcgat tatttggcga ggaggccgag
     1141 ggcctgctcc gcgcttttct tgctctctac aaagatggcc tcggccaaat ccggattgtg
     1201 gatccccttg gccttcacca cgaccgcgta gtccttgcgc gccttggcga ggaactcggc
     1261 ggccgcggtc acttcccgcc gatccttctt cgcggcagcc agagccgcct ctcccttctc
     1321 cagcgaggct cgagcggctt ccagggcccc tcccacctgc ccctgccaca tggccagcat
     1381 gtcgcggtac cccttgtcgt gacacccaat gcacttctcc gtgaccgtcg cgacgctgtg
     1441 tttcctggtc aggtcgtgac atccgacgca gtccaccttg ccggccatca cgttgggttc
     1501 ctttttcacc gactggaccg tctccagcgt ggccccgtac aggcccgcct gggcggcatg
     1561 acaggtcgtg cacctgtcgt tggcggcgct atggtggcag gcctggcaac cctctcgcgt
     1621 gatggtgact gccttgtgct tctccgcgga atggcagtcg gtgcacaccg ccccgtactg
     1681 attgaaatga ttctggtgcg gcacccgtgt cgtcttctcg aactgaacct cgggcttgaa
     1741 gatcacacct gcctggctat ggcaaagcgt ggcgcagtag ccacctcgga ccaagttctc
     1801 ctgggggatc atgatcgggc tcttcccaac gagccgcatg acccgatttg ccgaggcatt
     1861 taccttttgg agcagatcgg cggcatagaa gggattgtgg acgcccttcc cgttctccac
     1921 gaaatcgaag ttgaactccg cctcctccac gaggcgtctg gcatccgcca ggaccttcga
     1981 gtccttggcc ggcccctcca cggccttctt caccgcctcc atcttgggct tcaaagaggt
     2041 cagcatggtg tccagcgtgc gcttccaggc gtccagcatg ccggcgtact gctgtccatg
     2101 gcaggagacg caggccgcct gggatgccgt gaaggtctgc ccggagaatt gtcggctgtg
     2161 tccgtccttg accttgggtg tgatgtggca ggccacgcat tccacgcgtg ccgtgtacat
     2221 gtggctcggg atggtgggcg agccctttcc tccgatcccg agatacatag cccgctggcc
     2281 tccatgcttt gcctcgtgac aaccttcgca gcccacgctc attggttctt tcttcgtctg
     2341 gatctggtgc ttgatctccg tatgacaccg caggcacgcg atgttgtgac gcgtcacgtg
     2401 gaaatcgtgg aggaactcat ggtcgtcata cctggccagc ttctcgggct ggttgtggca
     2461 ggtgaagcac cgctcccgag gcgcctgccc atcgccctgc acggcgtcga gatggcagtt
     2521 ctggcatctg gccccacgag cctccacgaa ctccttgtga tcgaaggtga cgcccgcggc
     2581 caactggatc tgtcccttgg ggggttggtg gcacatggga cagccgccga tgggctgcag
     2641 gtctcgggcg gtctgcttcc ctttgaagtg acacaggaag caggttgcct cggtcacctc
     2701 gatgtgtgtg ccgacaacga tctgggagtg acagctcgtg cagcggagct ggcggccgcg
     2761 gcgcggcttt tccaggtgcg gccggtggtc gaagatgatc ccccgcttga acaagacctt
     2821 cccttccagg agccgcgtgg catggcactg cgaccgcagg caactggcgt cctcgatctc
     2881 ggcgaagggc ttggagctgt aggtctgcgt cgcccacttc gcgacctgag acagcgcctg
     2941 gtacttcacc accagggtat cgcggagacc cggggggtaa tgacagtcca cgcacgggac
     3001 gaagttgtgc ttggaggtct tccaggcctg gtaatacggc ttcatgatat ggcactggtt
     3061 gcacaggagc ggcgacgtgc tgacctccaa ggcggcaccc aggaggagga gcggcaggcc
     3121 gacgaccaag atgccgatca ccttgaaaaa gcggggacgg atgtgcaggc ggtcctgtcg
     3181 gcctggtggg gggacgtgta acccccagaa ggccatgaac ttctgccagt gggtctccag
     3241 atgaagatcc gggtcttggc ccgataggtt tcgccagcac cgccgaaacc atccgggctc
     3301 acccgataca ggtgcctcag gcatccttcc ccccctcgcc tgaaaaggtg aaattagcgc
     3361 actttatcac cagttcccga aaaaccgcaa gcctcgttcc tcgcgccggg agacaacgtc
     3421 aggagaaacg cacgattgaa cttggggtca ggaggcgggg ggatggcacg cctgacaggc
     3481 gcgccgatcc acagccagcg ctttgtgttt gctcaccgag tggcaggtgc cacaccccac
     3541 accggattcc gcgtgcagtt gatgcgggag cttcttcccc tccagcttca cttctgccgg
     3601 ga
//
LOCUS       DBNGZC010001089         3630 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1270283, whole genome shotgun sequence.
ACCESSION   DBNGZC010001089 DBNGZC010000000
VERSION     DBNGZC010001089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1270283"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="AC16G0_18770"
     CDS             complement(<1..954)
                     /locus_tag="AC16G0_18770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002029742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HIR5101705.1"
                     /translation="MGSEFRIEKDFLGTREVPADAYYGVQTLRGAENFPITGHRLNPA
                     LIRAMAIVKKAAALANRDTGYLPLRIANAIAAAADDVIAGKLHDQFIVDPIQGGAGTS
                     INMNANEVIANRALEILGEPKGNYAVVSPNTHVNMAQSTNDAFPTAVHIAALDVGREM
                     LAALRELRDAFAAKEKEFDGLIKMGRTHLQDAVPIRLGQEFGAYRRLLDRDIARILNV
                     GLHLVEVNMGATAVGTGLNADPAYIQQVVKYLAEFSGYPIVSAENLVDATQNTDSYTE
                     VSATLKVCMMNLSKIANDLRLMASGPRVGLYEIQLPARQPGS"
     gene            complement(1027..2361)
                     /locus_tag="AC16G0_18775"
     CDS             complement(1027..2361)
                     /locus_tag="AC16G0_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015767469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter family
                     protein"
                     /protein_id="HIR5101706.1"
                     /translation="MTLTMLLEFGVVLAAIVLGSRAGGVGLGFWGGVGVLVLTFGFGL
                     PPTSPPIDVMLIILAVIMAASVMEAAGGIDYLVSVAERIIRRNPRQITVVAPVVAYAF
                     TLGAGTGHVFYPLLPIIHDVARENGIRPERPIAISTIASQQAITASPVSAAMAAMIAL
                     FEPAGYGLTQIMLISIPATLLGVIVAALIQTRVGKELKDDPEYNRRLQAGEIEAPSGQ
                     QQKAARVALPPSARTSALLFLLGVVFVVVAGLFPGLRTLVPKADKMVSLDMATTIQII
                     MLSLAGLMLFVTKAPAARVTKTSVAQAGLTAVVGIFGLAWLGDTFIKGNEKVIIGAIG
                     DITTTYPITFALGLFFASVLLFSQAATTRALMPLGISLGIAAPNLMAMWPCVNGYFFL
                     PTYGTIIAAINFDRTGTTRIGRFVLNHSFMLPGLAATSCAVVIGLVLARLVF"
     gene            complement(2437..3246)
                     /locus_tag="AC16G0_18780"
     CDS             complement(2437..3246)
                     /locus_tag="AC16G0_18780"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR5101707.1"
                     /translation="MNRRQAGSTKAVILLLAVLLSLAAVSQAVADGLEDATRRGSLLV
                     GVKTDFPPFGYLDQAGTIQGFDVEIAEALARALFKEGGRLKPVVVTSGNRIPFLYSEW
                     IDLIIASMTITEERRQVLEFSEPYFLSGSLLLVPKDSPIKGLEDLTGKSVGIIEGAIQ
                     DKDLATVAPAARRVRFRQVPEAVQALKQKRVDAFCQDDVLVLTLAKENPNLRTAGKPF
                     LPRPYAIAARKGEVQLIRWVNEQLARMKIDWTYDRLWQKYFGDMGGPLLKP"
BASE COUNT          651 a         1139 c         1204 g          636 t
ORIGIN      
        1 gctgccgggc tggcgcgcgg ggagctggat ctcgtagagc cccacgcgcg gccccgaggc
       61 catgagtcgg aggtcgttgg caatcttcga caggttcatc atgcagacct tgagcgtggc
      121 cgacacctcg gtgtagctgt ccgtgttctg ggtcgcatcc acgagattct cggcgctcac
      181 gatgggatat ccgctgaact cggcgagata cttgacgact tgctggatgt aggccggatc
      241 cgcgttcagg ccggtcccca ccgcggtcgc acccatattc acctcgacca ggtgcaagcc
      301 gacattcagg atgcgcgcga tgtcgcggtc gagcaggcgg cggtaggcgc cgaactcctg
      361 accgaggcgg atggggaccg catcctgcag gtgggtgcgg cccatcttga tgaggccgtc
      421 gaattccttt tccttcgccg cgaacgcgtc ccgcagctcc cgcagggcgg ccagcatctc
      481 cctccccacg tcgagcgccg cgatgtgcac cgcggtcgga aaggcgtcgt tcgtcgactg
      541 cgccatgttg acgtgggtgt tcggggagac gactgcgtaa ttccccttcg gctcccccag
      601 gatctcgagg gcgcgattcg cgatcacctc gttggcgttc atgttgatgg aggtgccggc
      661 gccgccctgg atcgggtcca cgatgaactg gtcgtggagc ttgccggcga tcacatcatc
      721 cgcggcggcg gcgatggcgt tcgcgatccg cagcgggagg tacccggtgt cgcggttggc
      781 gagggccgcg gccttcttca cgatcgccat ggcgcggatg agcgcgggat tcaatcggtg
      841 gccggtgatc ggaaagttct cggcgccccg cagggtctgg acgccgtagt aggcgtccgc
      901 gggcacctcg cgcgtgccga ggaaatcctt ctcgatgcgg aactcgctgc ccatggaatc
      961 ctccttcctt ggttcggccg gaaatcgtcc gtcggggcca aatggagcgg ttcgccagcc
     1021 gggccgtcag aacacgagcc gggcgagcac gaggccgatc acgaccgcac agctcgtcgc
     1081 ggccaggccg gggagcatga aagaatggtt cagcacgaac cgcccgatgc gggtcgtgcc
     1141 ggtccggtcg aaattgatcg cggcaatgat ggtcccgtac gtggggagga agaagtagcc
     1201 attcacgcac ggccacatgg ccatgaggtt cggggccgca atgcccaggc tgatccccag
     1261 cggcatgagg gcccgggtgg ttgccgcctg gctaaagagc aggaccgaag cgaaaaacag
     1321 ccccagggca aacgtgatgg gataggtggt ggtgatatca ccgatggccc cgatgatgac
     1381 cttctcgttg cccttgatga aggtgtcacc cagccaggca agcccgaaga tgcccacgac
     1441 cgcggtgagt cccgcctgtg cgacgctggt cttggtcact ctggcggccg gtgccttggt
     1501 cacaaacagc atgagacccg ccagggagag catgatgatt tggatggtgg tcgccatgtc
     1561 cagggagacc atcttgtcgg ctttggggac gagcgtacgg agacccggaa acaggcccgc
     1621 caccacgacg aaaaccaccc cgagcagaaa gagcagggcg ctcgtcctgg cgctgggcgg
     1681 cagcgcaacc ctggcggcct tctgttgctg tcccgagggc gcctcgatct cgccggcctg
     1741 caatcgccgg ttgtactccg ggtcatcctt gagctcctta cccacccggg tctggatgag
     1801 ggcggccacg atcacaccca gcagcgtcgc cgggatggag atcagcatga tctgggtcag
     1861 gccgtatccg gccggctcga acagggcgat catcgccgcc atcgcggccg acacgggcga
     1921 ggcggtgatg gcctgctgcg aggcgatggt ggagatggcg atgggccgtt cgggtcggat
     1981 gccgttctcg cgggcgacat catgaatgat ggggagcaga ggatagaaga catgtcccgt
     2041 gccggctccc agggtgaagg cgtaggcgac gaccggggcc acaacggtga tctgccgggg
     2101 gttccgccgg ataatgcgct cggccacgct gaccagatag tcgatcccgc cagccgcctc
     2161 catcaccgag gcggccatga tcacggccag gatgatgagc atgacgtcga tggggggact
     2221 cgtggggggc aggccgaagc cgaaggtcag gaccagcacc cccacccctc cccagaaccc
     2281 gaggccgacg ccgccggccc ggctgccgag cacgatcgcc gccaggacca cgccaaactc
     2341 aagaagcatt gttagcgtca tcggtcgcgc tccttacgta ggtgacagcc ccgaaacccg
     2401 aggggacgcc gcagcaatga gcggaccgcc gccccgtcac ggcttgagca acggccctcc
     2461 catgtcgccg aaatatttct gccagagccg gtcgtaggtc cagtcgatct tcatgcgcgc
     2521 gagctgctcg tttacccacc ggatgagctg aacctcccct ttacgtgcgg cgatcgcata
     2581 gggacggggc aggaatggct tgccggccgt ccgcaggttg gggttctcct tggccagggt
     2641 gagcaccagg acatcgtcct gacagaaagc atccacacgc ttttgcttca gggcctggac
     2701 ggcctcgggg acctgacgaa accggacccg gcgggccgcg ggcgccaccg tggcgaggtc
     2761 cttgtcctgg atcgctccct cgatgatccc cacgctcttc cccgttagat cctccaggcc
     2821 cttgatgggg ctgtccttgg ggaccaagag gagggatccc gagaggaaat acggctccga
     2881 gaactccagc acctggcgcc gttcctcggt gatggtcatg ctggcaatga tcaggtcaat
     2941 ccattcgctg tagagaaacg gtatgcggtt gcccgacgtg accaccaccg gtttcagtct
     3001 gcctccttcc ttgaagaggg cccgggccag ggcctcggcg atctcgacat caaaaccctg
     3061 aatcgtccct gcctggtcca ggtagccgaa gggcggaaag tcggtcttca cgccgacgag
     3121 caggctgccc cgtcgcgtgg cgtcttccag cccgtcggca accgcctgac tgacggcggc
     3181 caggctcagg agcaccgcca gaaggaggat gaccgccttg gtggatccgg cctgtctgcg
     3241 attcatcgaa cgcctcctct gcgagggcat gaacgggcag catcaggtaa ggctgcccgc
     3301 ccgcatctca gggcatcggc gccgcgcccc tcttccgaac cagtacacga ccaggtgtcg
     3361 caattctggc ctgcgatcta caatggcccg atcaaactta cgaataatcg cggagattgt
     3421 ctattggacc ttggtccaat gagagtggcg tgggggggcc gatctcaacg ttgcgaagga
     3481 gacgaagagc cgttggatga gaaatgggaa gtgggaaccc cgtttcgggt cctgacgatc
     3541 gcgtgcagga tctccgacta ttcctgcacc tccttccagg acttgtccgg attgaattcc
     3601 accatgttgt gggcgatggc tgcgcttctc
//
LOCUS       DBNGZC010001090         2469 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1560425, whole genome shotgun sequence.
ACCESSION   DBNGZC010001090 DBNGZC010000000
VERSION     DBNGZC010001090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1560425"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="AC16G0_18785"
     CDS             <1..651
                     /locus_tag="AC16G0_18785"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5101708.1"
                     /translation="QPIYRDAVINGKFLAGMTIISLMLLGILLLISGMGLLILGVVPG
                     WEEVGRLFVYFLVSIVYIGFWLAASILCSVLFRSMATSALAALALWIFCAFFMPMGAS
                     LAADAMAPVPGGASTDAQAVIRHEELRDLVSSFSPITLYSQGTSIVLDPLRSTTRSLI
                     LMGPMERLSISRFQNPLPLLQSALIVAPHLVSLVAITAVCFGICYACFMRQEIRST"
     gene            706..1164
                     /locus_tag="AC16G0_18790"
     CDS             706..1164
                     /locus_tag="AC16G0_18790"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR5101709.1"
                     /translation="MSAWDPLVGLDALRREIDRTFDTFGSDRWPQNWRVAFLPGRAAR
                     QYPLINLSEDEGHLYVEALAPGVDPKTLDLSVLQGTLTIKGEKHGLAQVSAEAYHRNE
                     RAAGRFVRTIELPVEVSAAGVHADYRNGLLLVTLPKSEAAKPKQIPISVA"
     gene            1190..1588
                     /locus_tag="AC16G0_18795"
     CDS             1190..1588
                     /locus_tag="AC16G0_18795"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR5101710.1"
                     /translation="MAEKTVPSVQSEAGPARREETRAQERYITPPVDIFETKDGLTVV
                     ADLPGVEQKALDVRVADGVLTIQGKTAHVAPGRMVEQEFELLNFYRQFELPEEIDSDK
                     IAAELKHGVLALRLPKKEKAKPRKVEVSVS"
     gene            2154..>2469
                     /locus_tag="AC16G0_18800"
     CDS             2154..>2469
                     /locus_tag="AC16G0_18800"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5101711.1"
                     /translation="MSSGQDVTTETILLVDDDRFILQMCRDLLESEGYKTICLEEGGS
                     LQAVVENTQPSLILLDIMMPGSDGLTLCRQLRTGQTAFRGPIVICSAKHYRADKRVAR
                     EVG"
BASE COUNT          444 a          760 c          806 g          459 t
ORIGIN      
        1 cagcccatct accgcgacgc cgtgatcaac gggaagttcc tggcggggat gacgatcatc
       61 agcttgatgc tgctgggtat cctgctgctc atctcgggca tggggctctt gatcctgggg
      121 gtggtgccgg gctgggaaga ggtgggccgt ctcttcgtgt attttttggt cagcatcgtg
      181 tacatcgggt tctggctggc ggcctccatc ctctgctcgg tccttttccg gagcatggcg
      241 acctcggccc tggcggcttt ggccctctgg atcttctgcg ccttcttcat gcccatgggg
      301 gccagcctgg cggcagacgc catggccccg gtgcccgggg gtgcctcgac ggacgcgcag
      361 gcggtgatcc gccacgagga gctgcgggac ctggtctcca gcttctcgcc tatcaccctc
      421 tacagccagg ggacctccat cgtgctggac cccttgcgca gcacgacccg atcgctgatc
      481 ctgatgggcc ccatggagcg gctgtccatc agccgattcc agaacccgct gcccctgctc
      541 cagagcgcgc tgatcgtggc gccgcatctg gtctccctgg tggccatcac ggctgtgtgt
      601 ttcggaatct gttacgcctg tttcatgcga caggagatcc gctccacatg atgtgaacca
      661 gggcgcggcg acgcccgctc ccctcaccag aaaggaggaa cggtcatgtc tgcttgggat
      721 ccccttgttg gactggatgc tttgcggcga gagatcgacc ggaccttcga taccttcggg
      781 tccgatcgct ggccgcaaaa ctggcgggtg gctttcttgc cgggacgggc cgctcgccag
      841 tacccgctga tcaacctgag cgaggacgaa ggacacctct acgtggaggc gctggcgccc
      901 ggcgtggacc cgaagacact ggacctgtcg gttctccagg ggacactgac aatcaagggg
      961 gagaaacacg gtctggccca ggtgtcggca gaggcttatc atcgaaatga gcgggcggcg
     1021 gggcggttcg tccgcaccat tgaactgccc gtagaggtga gtgcggctgg agtgcacgcg
     1081 gattatcgaa acggattgct cctggtgacg ctgccaaaga gcgaggcagc gaagcccaag
     1141 cagatcccga tcagcgtggc ctgaagccgg gagaaaggag gtgacaacca tggcggagaa
     1201 gacggttccc agcgtgcaaa gcgaggcggg tcccgcccgg cgcgaggaga cccgcgccca
     1261 ggagcgctac atcacgcctc cggtggatat cttcgagacg aaggacggcc tcacggtggt
     1321 ggcggacctt cctggggtgg agcaaaaggc cctggatgtt cgagtggcag acggcgtctt
     1381 gaccatccag ggaaagacgg cacacgtggc ccccgggagg atggtcgagc aggagttcga
     1441 gttgttgaac ttctaccggc agttcgagtt gccggaagaa atcgattccg acaagattgc
     1501 agccgaattg aagcacggag tcctggccct ccgccttccc aagaaggaaa aagccaagcc
     1561 gcggaaggtc gaggtcagcg tgagctagtg tggtgaatcg gaagcccgta gacaaattcc
     1621 cggcccgagg ggcacccctc gggccttgga atttgttggc gaatctctgc ctcagcacac
     1681 tagcgtctgt ccgtgcggct ttacgatttc ggccggatat ctcgctcacg cagttctgat
     1741 ccacgagcca gtccaccggt ggcaggctgg cagtcaccgc gccgatacgc acggctctct
     1801 ggccagggga gaggaagccc gcttccatga aaacctctgg cgccgggagc acggtcttcg
     1861 cacgttcccg aggctgggat ctccaactcg ccgcggctcc tgccatccgt ctgttttcac
     1921 caccgtgccc cgctgcacgg gttgtcttac cgatatgcgc tgagaaaacc tgcacttgac
     1981 acttcggcgc gctcaggcta tgaaagggac agttgccgtc agtttcggag gatggaccag
     2041 cttcgtcgcg ccgtcggctt ctcaccgaag gccaggagga atggcgcgcg ccgtccggga
     2101 cacgtcggac gcaccgcaga atgatgcacg aggtggaatc cggtggaggg tgcatgtcca
     2161 gcggacagga cgtgaccaca gagacgatcc tcctggtgga cgacgaccgc ttcatcctcc
     2221 agatgtgccg ggacctcctg gagtcggagg ggtacaagac catctgcctg gaggaggggg
     2281 gctcccttca ggccgtcgtg gagaacaccc agccgtccct tatcttgctg gacatcatga
     2341 tgcccggcag cgacggcctc acgctctgcc ggcagctccg gactggccag accgctttcc
     2401 gaggtcccat cgtcatctgc tcggccaagc actaccgcgc ggacaaacgt gtcgcccgcg
     2461 aggtgggcg
//
LOCUS       DBNGZC010001091         2764 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_583651, whole genome shotgun sequence.
ACCESSION   DBNGZC010001091 DBNGZC010000000
VERSION     DBNGZC010001091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_583651"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /gene="coxB"
                     /locus_tag="AC16G0_18805"
     CDS             <1..840
                     /gene="coxB"
                     /locus_tag="AC16G0_18805"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR5101712.1"
                     /translation="FLTVIFFAIKYRRRSEKERPRPIHGSLALEIVWTVIPLGLTMIM
                     FSWGAWLYFSMSRPPADAMEIFVVGKQWMWKLQHPDGQREINELHVPVGRPVKLTMTS
                     EDTIHSFFVPEFRVKKDVVPGRYSTVWFQATKPGEYHLFCTQYCGTSHATMIGRIVVM
                     PQDAFEGWLRGGGTGVSLAAAGEKLFQQLACVSCHVSSGTGVGPTLVGIFGKPVKLTT
                     GQTVTADEAYIRESILDPTAKIVAGYAPVMPPFRGQVNEDQLVQLLAYIKSLATEEKA
                     KAQ"
     gene            840..2456
                     /gene="ctaD"
                     /locus_tag="AC16G0_18810"
     CDS             840..2456
                     /gene="ctaD"
                     /locus_tag="AC16G0_18810"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HIR5101713.1"
                     /translation="MSTAASDPTVNYLNVDHSVKSWLLTTDHKRIAILYLLAATLFFL
                     LGSVYAGLIRLNLMTPYGDLIQAETYNKLFTMHGITMIFFFLIPSIPAVMGNFLIPMM
                     IGARDVAFPRLNLLSWYVYVLGGTFTLVAATLGGVDTGWTFYAPYSTVYSNTNVIITA
                     LGVFITGFSSILTGLNFIVTIHKLRAPGMTWFRMPLFIWAHYATSLIMILGTPVIAIT
                     IFLVAIERGLHLGIFDPALGGDPILFQHLFWFYSHPAVYIMVLPSMGVVSELIACFSR
                     KRIFGYEFVAFSSLAIAILGFLVWGHHMFVSGQSVYAGMIFSLLSFLVAIPSAIKVFN
                     WTATMYKGSLAYHAPMLYAMGFIGLFTIGGLTGLFLATLGADVHVHDTYFVIAHFHYI
                     MVGGAMMGYLGGIHFWWPKMTGRMYPEAWSRAAGLILFVGFNLTFFPQFILGFLGMPR
                     RYHVYPPEFQVLNVMSSAGATILGVAYVIPLIYLLWSLRYGQVAGPNPWGAAGLEWTT
                     PSPPPTHNFEKVPIVTEEAYAYGPTEAEVG"
     gene            2449..>2764
                     /locus_tag="AC16G0_18815"
     CDS             2449..>2764
                     /locus_tag="AC16G0_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HIR5101714.1"
                     /translation="MANTESPLQHHFAGLEQQHEASSLGMWAFLVTEIMFFGGLFTLF
                     IVYVTAYAEAFADGSHHLDIVLGATNTAVLIGSSFTMAMAVRSAQLGKQKPLLGFLLL
                     TML"
BASE COUNT          467 a          930 c          787 g          580 t
ORIGIN      
        1 ttcctgacgg tgatcttctt cgccatcaag tatcgcaggc ggtcggagaa agaacggcct
       61 cgccccattc acggctcctt ggcccttgag atcgtctgga ccgtcatccc gctcggcctg
      121 accatgatca tgttcagttg gggcgcctgg ctctacttca gcatgtcccg cccgccggcg
      181 gacgccatgg agatcttcgt cgtcggcaag cagtggatgt ggaagctcca gcaccccgac
      241 ggccagcggg agatcaacga gcttcacgtc ccggtggggc ggcccgtgaa gctgaccatg
      301 acctccgagg acacgatcca cagcttcttc gttccggagt ttcgcgtcaa gaaggacgtg
      361 gtgccgggac gctacagcac cgtgtggttc caggccacca agcccgggga gtaccacctc
      421 ttctgcaccc agtactgcgg gaccagccac gccaccatga tcggccggat cgtggtcatg
      481 ccgcaggatg cattcgaggg ctggctcagg ggcggcggca ccggcgtgtc gctggcggcg
      541 gccggggaga agctcttcca gcagctcgcc tgcgtcagct gccatgtttc cagcggcacc
      601 ggcgtggggc ccacgctggt ggggatcttc ggcaagccgg tgaagctcac gacggggcag
      661 acggtgaccg ctgacgaggc ctacatccgc gagtcgatat tggatcccac ggccaagatc
      721 gtggcgggat acgcaccggt catgcccccg ttcaggggac aggtcaacga ggaccaattg
      781 gtccagctcc tggcgtatat caagtctctg gcgacagaag agaaggcgaa ggcacagtga
      841 tgagcaccgc tgcatcggac ccaaccgtca attacctcaa cgtcgaccac agcgtgaagt
      901 cgtggctctt gaccaccgac cacaagcgca tcgcgatcct ttacctgctc gccgcgacgc
      961 tcttcttcct gctcggttcg gtgtacgccg ggctcatccg gttgaatctg atgaccccct
     1021 acggcgatct catccaggcg gagacctaca acaagctctt caccatgcac ggcatcacca
     1081 tgatcttctt cttcctgatc ccctccatcc cggccgtgat gggaaacttc ctcattccca
     1141 tgatgatcgg agcgcgtgac gtcgccttcc cccgcctcaa cctgctgagc tggtatgtgt
     1201 atgtcctcgg cggcaccttt accctcgtgg cggcgaccct ggggggcgtg gacaccggtt
     1261 ggaccttcta cgccccttac agcactgtct attccaatac gaacgtcatc atcacggcgt
     1321 tgggggtctt catcacaggc ttctcctcga tcttgaccgg gctgaacttc atcgtcacga
     1381 tccacaagct gcgggccccg ggcatgacct ggttccggat gccgctcttc atttgggcgc
     1441 attacgccac cagcctcatc atgatcctgg ggaccccggt catcgccatc acgatcttcc
     1501 tggttgccat cgagcgaggg ctgcatctcg ggatcttcga tcccgctctg ggcggggacc
     1561 cgattctctt ccagcatctg ttctggttct actcgcaccc tgccgtgtac atcatggtcc
     1621 tcccctccat gggcgtcgtc agcgagctga tcgcctgctt ctcccgcaag cggatcttcg
     1681 gctacgagtt cgtcgccttt tccagtctgg ccatcgccat cctgggcttc ctggtctggg
     1741 ggcaccacat gttcgtgagc ggtcagtccg tgtatgcggg catgatcttc tccctcctga
     1801 gcttcctggt cgccattccc tccgcgatca aggtcttcaa ctggaccgcc accatgtaca
     1861 agggatccct cgcctaccat gcccccatgc tctacgccat gggcttcatc gggctgttca
     1921 ccatcggcgg gctcaccggc ctcttcctcg ccaccctggg ggcggacgtc cacgtgcacg
     1981 acacctactt cgtcatcgcc cattttcact acatcatggt tgggggggcc atgatgggat
     2041 acctgggcgg aatccacttc tggtggccca agatgaccgg gcgcatgtat ccagaagcct
     2101 ggtcgcgggc agccggtctg atcctgttcg tgggattcaa cctgaccttc tttccccagt
     2161 tcatcctggg cttcctggga atgccgcgcc gttaccacgt ctaccccccg gaattccagg
     2221 tgctgaacgt gatgtcctcg gcgggggcaa ccatcctcgg cgtggcctac gtgatcccct
     2281 tgatctatct tctgtggtcg ttgcggtatg gccaggtggc gggccccaac ccgtgggggg
     2341 ccgcggggct cgagtggacg acgccatccc caccgccgac gcacaacttt gagaaggtcc
     2401 cgatcgtgac cgaagaagcg tatgcctatg ggcccacgga ggcggaagtt ggctaacacc
     2461 gagtcgcccc tgcaacacca tttcgccggc ctggagcagc agcacgaggc ctccagcctg
     2521 ggaatgtggg ccttcctggt caccgagatc atgttcttcg gcggcctctt caccctgttc
     2581 atcgtgtacg tgaccgcgta cgccgaggcc ttcgccgatg gaagccacca cctcgatatc
     2641 gtgctcggcg ccaccaacac ggcggtactg atcggcagca gcttcaccat ggccatggca
     2701 gttcgaagcg ctcaactggg caaacagaag cccctcctcg ggttcctcct gctcacgatg
     2761 ctcc
//
LOCUS       DBNGZC010001092         2101 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_878283, whole genome shotgun sequence.
ACCESSION   DBNGZC010001092 DBNGZC010000000
VERSION     DBNGZC010001092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_878283"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="AC16G0_18820"
     CDS             <1..914
                     /locus_tag="AC16G0_18820"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="complex I 51 kDa subunit family protein"
                     /protein_id="HIR5101715.1"
                     /translation="RGEFVKGAQVLGRALDEAYAHGYLGQNILGSDFNLEVLVHRGAG
                     AYICGEETGLLESLEGKRGQPRMKPPFPAISGLYRCPTVVNNVETLSNIPHIVLRGAD
                     WYAGIGPPKSAGPRLFSVSGHVARPGNYELPMSITLRELVFEHAGGMRDGRHLKAVIP
                     GGSSAPVLTEQHLDLTMDFDSLAAAGSMGGSGGVIVMDDTTCMVRVGEIVSRFYHHES
                     CGQCTQCREGTAWLHKVLRRIEHGQGRKEDLDLLLDMCDNMKGKTICVLSDAAAMPIE
                     SYLRHFQDEFERHVLERACPFEGRGPF"
     gene            997..>2101
                     /locus_tag="AC16G0_18825"
     CDS             997..>2101
                     /locus_tag="AC16G0_18825"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024900.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HIR5101716.1"
                     /translation="MPTLKINGIEVTVPAQTTIIEAARAAGIEVPRYCYHPGLSVVGQ
                     CRMCQVEVEKVPKLAIACNTAVADGMVVSTNSPKVEKARRAVLEFYLLNHPLDCPICD
                     KGGECPLQDYTLTYGPGESRSVEEKVKRAKHRVLGPHIVFDAERCILCTRCVRFCNEI
                     PKTGELGVFSRGDRSEINLFPKKVLDNAYSGNVIDLCPVGALTSREYRFRSRPWDLVR
                     HTDSVCPLCSAGCNVILDVRHRQRGAELLRIRPRENPAVNRWWMCDEGRFNFSYLQDP
                     RRLRVPLWRESDRQMEVGWEDLLHRLARAVRRVALRPGEAVVGCIVSSRHTNEELFLI
                     RRFFREILRTPHLDFRVRSLQFQDADASEDGILR"
BASE COUNT          350 a          695 c          706 g          350 t
ORIGIN      
        1 gccggggcga attcgtgaag ggggcccagg tcctcggacg ggccctggat gaggcctacg
       61 cccacggcta cctgggccag aacatcctgg gcagcgactt caacctggag gtcctggtgc
      121 atcggggggc gggggcctac atctgcggcg aggaaaccgg gctcttggag tcgctggagg
      181 gcaagcgcgg ccagccgcgg atgaagccgc ccttccccgc catctcgggt ctgtaccggt
      241 gtcccacggt ggtcaacaac gtcgagacgc tcagcaacat cccccacatc gtcttgcgcg
      301 gggccgactg gtatgcgggc atcggcccac ccaagagcgc cggaccgcgc ctgttttccg
      361 tgagcggtca cgtggcccgg cccggcaact acgagctccc catgagcatc accctgcggg
      421 agctggtctt cgagcacgcc ggcggcatgc gggacggccg gcacctgaag gccgtgatcc
      481 cgggagggtc ctcggccccc gtgctcacgg agcagcacct ggacctgacc atggatttcg
      541 actccctggc ggccgccggg tccatggggg gctcgggcgg cgtgatcgtc atggacgaca
      601 ccacctgcat ggtccgggtg ggcgagatcg tctcgcgctt ctaccaccac gaatcctgcg
      661 gccagtgcac gcagtgccgc gagggcaccg cctggctgca caaggtcctg cggcggatcg
      721 agcacggcca ggggcggaaa gaggatctcg acctgctcct ggatatgtgc gacaacatga
      781 aggggaagac catctgcgtc ctgagcgacg ccgcggccat gcccatcgag agctacctcc
      841 ggcatttcca ggacgagttc gagcggcatg tcctggagcg ggcctgcccc ttcgaagggc
      901 gaggtccgtt ctgatggcac ctgggcaatc agcaactcgt ctgtcttctg attgcctgat
      961 tgccagaata ccgggccaat catcaaggat gagcggatgc cgacgttgaa gatcaatggc
     1021 atagaggtca ccgtccccgc ccagacgacc atcatcgagg cggccagggc ggcggggatc
     1081 gaggtccctc gctactgcta tcaccccggt ctgagcgtgg tggggcagtg ccggatgtgc
     1141 caggtggaag tcgagaaggt gcccaagctc gccatcgcct gtaacaccgc ggtggccgac
     1201 ggtatggtcg tctccacgaa ctcacccaag gtggagaagg cgcgccgggc ggtgctggag
     1261 ttctatctcc tgaaccaccc cctggactgt cccatctgcg acaagggggg cgagtgcccc
     1321 ctccaggatt acacgctcac ctacggaccc ggggaaagcc ggtccgtgga ggagaaggtc
     1381 aagcgggcca agcatcgggt cctgggccct cacatcgtct tcgacgcgga gcggtgcatc
     1441 ctctgcaccc gttgcgtccg cttctgtaac gagatcccca agaccgggga gctgggggtt
     1501 ttctcccgag gcgaccgctc cgagatcaac ctcttcccga aaaaggtgct ggacaacgcc
     1561 tactccggca acgtgatcga cctgtgcccc gtgggggccc tgacctcccg cgagtatcga
     1621 ttccgttccc ggccctggga cctcgtgcgc cacaccgaca gcgtctgccc cctctgcagc
     1681 gcgggctgca acgtcattct ggacgtccgc caccggcagc ggggcgcaga actgctccgc
     1741 atccggccgc gggagaatcc ggcggtcaat cggtggtgga tgtgcgacga ggggcggttc
     1801 aacttctcct acctgcagga tccgaggcga ctccgcgtcc cgctgtggcg cgagagcgac
     1861 cggcagatgg aggttggctg ggaggatctg ctgcatcggc tggcgcgggc ggtccggcgc
     1921 gtcgccttac ggcccgggga agcggtggtc gggtgcatcg tctcctcccg ccacaccaac
     1981 gaggagctgt tcctgatccg acggtttttc cgggagatcc tccggacgcc gcatctggat
     2041 ttccgtgtcc gctccctgca gttccaggac gccgacgcct ccgaggacgg aatcctgcgg
     2101 c
//
LOCUS       DBNGZC010001093         1773 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1756404, whole genome shotgun sequence.
ACCESSION   DBNGZC010001093 DBNGZC010000000
VERSION     DBNGZC010001093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1756404"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="AC16G0_18830"
     CDS             <1..191
                     /locus_tag="AC16G0_18830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020732616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101717.1"
                     /translation="LRGLKENVIMGRLVPAGTGMRWYRETRIATPEVAPPRPEEPEPT
                     AHQEAAAELLQAMDVNLP"
     gene            281..658
                     /gene="rpsL"
                     /locus_tag="AC16G0_18835"
     CDS             281..658
                     /gene="rpsL"
                     /locus_tag="AC16G0_18835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009016331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR5101718.1"
                     /translation="MPTITQLVRRGRRQIPSKEKAPALQACPQKRGVCVRVYTTTPKK
                     PNSALRKVARVRLTNQIEVTTYIPGIGHNLQEHSIVLIRGGRVKDLPGVRYHVIRGAL
                     DTAGVADRRQSRSLYGAKRPKAS"
     gene            762..1232
                     /gene="rpsG"
                     /locus_tag="AC16G0_18840"
     CDS             762..1232
                     /gene="rpsG"
                     /locus_tag="AC16G0_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR5101719.1"
                     /translation="MPRRKVAEKRDVLPDPVYGNKLVTKFVNSMMGQGKRNTAERIFY
                     GCMHLIEDRTKMDAQKLFKQAVDNVKPVLEVKSRRVGGATYQVPVEVRAERRTSLALR
                     WIIGFARKRTEKTMPERLAAELIEAANNRGSSVKKREDTHKMAEANKAFAHYRW"
     gene            1297..>1773
                     /locus_tag="AC16G0_18845"
     CDS             1297..>1773
                     /locus_tag="AC16G0_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR5101720.1"
                     /translation="MAETLSLDRTRNIGIMAHIDAGKTTTTERILYYTGKTHKIGEVD
                     EGSTEMDWMEQEKERGITITAAATTCYWNEHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VAVFDAVAGVEPQSETVWRQAEKYAVPRIAFVNKMDRPGADYFGVVSQIQKDLGANA"
BASE COUNT          367 a          546 c          604 g          256 t
ORIGIN      
        1 agctgcgagg cctgaaggag aacgtgatca tgggtcgcct ggtcccggcg ggcaccggca
       61 tgcgctggta tcgcgaaacg cgcatcgcca cgcccgaggt ggcgccgccg cggcccgaag
      121 agcctgagcc gactgcgcat caagaggccg cggcagagct gttgcaggcc atggacgtga
      181 atctcccatg atcgtgctgg ccagaaaccc cttgacaaac ggagacccag cacgtaaact
      241 ccgccggttc tctacgctct gtaggaatta aggacgatcc atgccgacca tcacacagct
      301 cgtgcggcga gggcgccggc agatccccag caaggaaaag gcgccggccc tccaggcctg
      361 tccgcagaag cgaggggtgt gcgtccgcgt ctataccacg acgcccaaga agccgaactc
      421 ggccctgcga aaggtcgcgc gcgtgcgctt gaccaatcag attgaggtga ccacctatat
      481 cccggggatc gggcacaacc tgcaggaaca ctccatcgtc ctcatccggg gtgggcgcgt
      541 caaggacctc ccgggtgtcc gctaccatgt gatccggggc gctttggaca cggccggggt
      601 ggccgaccgg cggcagagtc gctccctcta cggggccaag agacccaagg ctagctaagc
      661 aaaccggcac tgaagacagg gggcccactt gccctccagt gccggttttc ttctctgtgg
      721 caggtccccc gagcggcggg ccggcgagtg aggagtcggg aatgccgaga cggaaggtcg
      781 ccgagaagcg ggatgtcctg cccgatcccg tgtacggcaa caagctggtc acgaagttcg
      841 tgaactccat gatggggcag gggaagcgga acaccgccga gcgcatcttc tacggctgca
      901 tgcacctcat cgaggatcgg accaagatgg atgcgcagaa gctcttcaag caggcggtgg
      961 acaacgtgaa gccggtgctg gaggtcaagt cccgccgggt ggggggcgcg acctatcagg
     1021 ttccggtgga ggtgcgggcc gagcgccgga cctccctggc cctgcgctgg atcattggat
     1081 ttgcccgcaa gcggaccgag aagacgatgc cggaacggct ggcggcggaa ctgatcgagg
     1141 cggccaacaa ccgcgggtcc tcggtcaaga agcgtgaaga tacccacaag atggcggagg
     1201 ccaacaaggc tttcgcgcat taccgttggt aatgggcagg atcttcgaac acgatcgctg
     1261 gtaaccggta cacccggaag cggcaaggga accaccgtgg ctgagacgct ctcgctggat
     1321 cgaacccgga acatcggcat catggcgcac atcgatgccg gcaagacgac cacgaccgag
     1381 cggatcttgt actacaccgg caagacccac aagatcggcg aggtggacga gggttcgacc
     1441 gagatggact ggatggagca ggagaaggag cgggggatca ccatcaccgc ggcggccacg
     1501 acgtgctact ggaacgagca ccgcatcaac atcatcgaca ccccgggcca cgtggacttc
     1561 accgtggagg tggagcgctc cctgcgcgtg ctggacggag ccgtggcggt gttcgacgcg
     1621 gtggccggcg tggagccgca gtcggagacg gtctggcgcc aggccgagaa gtacgccgtg
     1681 ccccgcatcg cgttcgtcaa caagatggat cgcccgggcg ccgactactt cggcgtcgtg
     1741 tcccagatcc agaaggacct cggggcgaac gcc
//
LOCUS       DBNGZC010001094         3548 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1365733, whole genome shotgun sequence.
ACCESSION   DBNGZC010001094 DBNGZC010000000
VERSION     DBNGZC010001094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1365733"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..549
                     /locus_tag="AC16G0_18850"
     CDS             133..549
                     /locus_tag="AC16G0_18850"
                     /inference="COORDINATES: protein motif:HMM:NF023395.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR5101721.1"
                     /translation="MTECIFCKIVAGEVKGNVVYENESVMAFLDIRPASRGHVMVIPK
                     VHADRLDVLEDGNVGSLFTGVKAVMRLLEKALRPEGMNIGWNHGWAAGQHVNHLHVHI
                     IPRYAGDGGGGIQSLIRSSAKEDLSSIAAQIRAAAG"
     gene            884..1438
                     /locus_tag="AC16G0_18855"
     CDS             884..1438
                     /locus_tag="AC16G0_18855"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:1094..1096,aa:Sec)
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR5101722.1"
                     /translation="MHIARRIIRGVVLATVACLLLAGTAQALQVGQKAPDFTLPAPDG
                     KQVKLDDLLGKGPVVIFTFIQAFGGVUTKEINGFNAALPQFEAVNAQVVGVSADAGGT
                     LLSFTKTNDIRFPLLADFSRKMLPAYDAMVTDEKSPIYRYGKRAYFILDKQGTIKYIK
                     VMDNPLDLLAPEEVLKAVKESGAA"
     gene            1435..1650
                     /locus_tag="AC16G0_18860"
     CDS             1435..1650
                     /locus_tag="AC16G0_18860"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR5101723.1"
                     /translation="MRSSFLALGLTILLVGAPAVWASPDFASMQIAPYDPPKAAPGLS
                     LPDLQGKTVNLADLKGNVVLIWFWATW"
     gene            1663..1977
                     /locus_tag="AC16G0_18865"
                     /pseudo
     CDS             1663..1977
                     /locus_tag="AC16G0_18865"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
     gene            complement(2074..2790)
                     /locus_tag="AC16G0_18870"
     CDS             complement(2074..2790)
                     /locus_tag="AC16G0_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HIR5101724.1"
                     /translation="MIFRAIAVVVMVCCVSSPASAQQLDQLFKGLGIGQQSGLTDAKI
                     GSGLKEALKVGTENTVNLTGRPDGYFMNQAIKILMPPKLQTLEKGLRTVGYGPQVDEF
                     ILSMNRAAEQAAPSAKKIFWDAIGEMTFDDARKILSGGDTAATEYFKGKTTDRLTTAF
                     RPVVEKATNEVGVTRQYKDLVGRAQAIPFLKVESLDVDNYVVGKSLDGLFYVLAGEER
                     KIRTDPAARVTDLLKEVFKK"
     gene            3046..>3548
                     /locus_tag="AC16G0_18875"
     CDS             3046..>3548
                     /locus_tag="AC16G0_18875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5101725.1"
                     /translation="MNTRQARAKLVTACAAHFLHDGFTDLLFVLFPVWARAFSLSFAQ
                     VGLLKTCYSGAMALFQVPAGLLAERWGEARLLAAGTALVGAGFLLLGTAGGFGLLLVF
                     LLGGGLGASVQHPLGSSLVSRAYEEGSRRVALGTYNFSGDFGKMVLSTCAGLVVAWAG
                     WRIAVRGV"
BASE COUNT          623 a         1104 c         1134 g          687 t
ORIGIN      
        1 aaagaggatg cccggacctc ccggcccctg gagcatcgag ggcttagcgc ttgcgcagca
       61 ggagagcggg cggatacaat gtcgcagcgt atccctgtcg agatggcgac ggagccgaga
      121 aggagcgaac gcatgaccga gtgtatcttt tgtaagatcg ttgcgggcga ggtgaagggg
      181 aatgtggtgt acgagaacga aagcgtcatg gcgttcctgg acatccgacc cgcgtcgcgg
      241 gggcacgtca tggtgatccc gaaggtgcac gccgaccgac tggacgttct ggaggacggg
      301 aacgtcggat cgctcttcac cggggtgaag gccgtgatgc gcttgctgga gaaggccctc
      361 cggcccgagg ggatgaacat cggctggaac cacggctggg ctgccggaca gcacgtgaac
      421 cacctgcacg tccacatcat cccgcgctac gccggcgatg gcggcggcgg catccagtct
      481 ctgatccgaa gctcggccaa ggaggatttg tccagcatcg ccgcgcagat tcgggcggcg
      541 gcagggtagc tgccaggcct ggctcggtcg gatctcagat ctacgccctg gaagactcta
      601 cgtctcaaag gtgccagtct gtcctgcgtc tctgcaccta tctacccggc ttttcgcttc
      661 tctctcaccg atggcgtgat cccagggtct ccgaacaggg tttcagccgg gtatatgacg
      721 ctgcgggttc cacgcgtggg aacctatcgc gcccaaggat agttgtaggt tgtggggtgg
      781 aagcatcctt gatccccgct tgcggcagcc gaaaatgatc tttgcgaatc gcgcttagcg
      841 tggcaggatc atctcggcaa taacctcaca aaggaggggg cacatgcaca tagcacgtcg
      901 aatcattcgg ggagtcgttc tcgccacggt ggcctgcctg ctcctggccg gcacggcgca
      961 ggccctccag gtgggccaga aggcgccgga cttcacactc ccggccccgg acggaaagca
     1021 ggtcaagctg gacgacctcc tgggcaaggg cccggtggtc atcttcacct tcatccaggc
     1081 tttcggcggt gtctgaacga aggagatcaa cggcttcaac gctgcgctgc cgcagttcga
     1141 agccgttaac gcccaggtcg tgggcgtgag cgccgatgca ggcgggacgc tgctttcctt
     1201 cacaaagaca aacgacatcc gcttcccgct cctcgccgat ttctctcgca agatgctgcc
     1261 ggcctacgat gccatggtga cggacgagaa gagtccgatc taccgctacg gcaagcgcgc
     1321 ctacttcatc ctcgacaagc aggggaccat caagtacatc aaggtgatgg ataatcccct
     1381 ggacctcttg gcgcccgaag aggtgctgaa ggcggtgaaa gagagtggcg cagcatgaga
     1441 agcagttttc tggcgctcgg cttgacgata ttgctcgtcg gggcgccggc cgtctgggcc
     1501 agcccggatt tcgcctcgat gcagatcgcc ccgtacgacc cgcccaaggc ggcgcctggc
     1561 ttgtcgcttc cggacctcca ggggaagacg gtcaatctcg cggacctcaa gggcaatgtg
     1621 gtcctcatct ggttctgggc cacctggtga ccagattgcc gggaggagtt gccttccgtc
     1681 aataagcttt acaccgaatt caagaaccag gggctagaga tcctgctggt gaatatccgg
     1741 gagggccgcg acttggtcga gcggactgcg atggaacgcg gctacgttgc ccccatcttg
     1801 ctcgaccaga gcggggatac ggccgggagg caatggggcg tgtgggggac ccccacagcc
     1861 tacatcatcg atcgccaagg tcgactcaat gggcggatcg tgggggcacg cgactggagc
     1921 aagcccgcgg cccgtgaatt gatccgagcg ctgcttcagg cggacgcgaa gtcctgaagt
     1981 gcaacatggc gcgggggggg cggagcaact ccgccccacc acctctcccg tcatcccggc
     2041 tatcgattct gaaccgcctc tgccatccga aggttatttc ttgaatactt ccttcagaag
     2101 atctgtcacc cgcgcggcgg ggtccgtgcg gatcttgcgc tcctctcccg cgaggacgta
     2161 gaagagtcca tccagggact tccccacgac gtagttatcc acgtccaggg attcgacctt
     2221 gaggaagggg atggcttggg cgcggccgac cagatccttg tactgccgcg tgacgcccac
     2281 ctcgttggtg gccttctcca ccaccggccg gaaggcggtc gtcagcctgt ccgtggtctt
     2341 ccccttgaaa tactccgtgg ccgcggtgtc gcctccggag aggatcttgc gggcgtcgtc
     2401 gaaggtcatc tcgccgatgg catcccagaa gattttcttg gcggacggag cggcttgctc
     2461 ggcggcccgg ttcatgctca ggatgaattc atccacctgc ggcccatagc cgacggttcg
     2521 caaacccttc tccagggttt ggagcttcgg cggcatcagg atcttgatcg cctggttcat
     2581 gaagtacccg tctgggcgtc ccgtcaggtt gaccgtgttc tcggtcccga ccttgagcgc
     2641 ctccttgagg ccggagccga tcttggcgtc cgtgaggccg ctttgctggc cgattcccag
     2701 ccccttgaag agctgatcga gttgctgggc cgatgcaggc gaactcacgc agcacaccat
     2761 cacaaccacg gcgatcgcac ggaatatcat ggccgcaact ccttcccccc tcgaacacgc
     2821 gtgatcaacc gaccgatctt ctcgaccaga aggccagacc tttgacgcga cggcaccgag
     2881 cctaccacaa atccatctca tcgggggacg ttttcctgcg gcggcgccga tcacccccgg
     2941 ccctcggccg aaggaggcgg cggtgatcga gcatgagggg gacggcgtcg aatccacctc
     3001 gctttcgctt gacaggagtg caggcggctt gaaagaatgc cgcgcatgaa cacgcgtcag
     3061 gcgcgggcga aactggtgac agcctgcgcc gcccactttc tgcacgacgg attcaccgat
     3121 ctcctcttcg tgctctttcc cgtctgggcg cgagcgttct cgctttcatt cgcccaggtg
     3181 gggctactca agacatgcta ttccggggcg atggccctgt tccaggtccc ggcgggtctc
     3241 ctggccgagc ggtgggggga ggcccgcctg ctggcagccg ggaccgcgct ggtcggagca
     3301 ggctttctcc tcctcggcac ggcaggaggc ttcggcctcc tcctcgtgtt tcttctcggc
     3361 ggcggtctcg gggcgtcggt ccagcatccc ctgggctcct ctctggtatc gcgtgcctac
     3421 gaggaaggat cacggcgcgt ggcgctcggg acctacaatt tttccggcga tttcggcaag
     3481 atggtcctgt cgacttgcgc cggcctcgtc gttgcctggg cagggtggcg tatcgcggtg
     3541 cgaggcgt
//
LOCUS       DBNGZC010001095         3547 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_878789, whole genome shotgun sequence.
ACCESSION   DBNGZC010001095 DBNGZC010000000
VERSION     DBNGZC010001095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_878789"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(263..595)
                     /locus_tag="AC16G0_18880"
     CDS             complement(263..595)
                     /locus_tag="AC16G0_18880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5101726.1"
                     /translation="MEKAKRKRLEAKGWKVGTAAEFLGLTPEESAYVELKVALGSKLR
                     ELRNRKRLSQVELAKRVGSSQSRVAKLEAADPSVSLDLLIRSLIALGVSRQDLARVIA
                     SPIPRAAA"
     gene            complement(613..942)
                     /locus_tag="AC16G0_18885"
     CDS             complement(613..942)
                     /locus_tag="AC16G0_18885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002763716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HIR5101727.1"
                     /translation="MSREDKPLVWLHGEVKTPPFSPAGRVEVGVLLRRLQRGESLGLP
                     RSRPMPSIGSRCHELRVTDQGVAWRLIYRADPDAIIILEVFSKKTRHTPQDVITICIQ
                     RLRAYDS"
     gene            complement(1119..2255)
                     /locus_tag="AC16G0_18890"
     CDS             complement(1119..2255)
                     /locus_tag="AC16G0_18890"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HIR5101728.1"
                     /translation="MKVTDLETYLVDTKGLLKWVFVKLTTDEGIVGYGEAGNAFRERA
                     QAAAIQELKPLVMGRDPFQIRSLWSQVYKTHLDVRGPSSLHVSALGAVEVALWDIVGK
                     ALRASIYNLLGGKCRDRVRAYLHAQVWGGEAEPGPEVYVELARARVAEGFSAIKFDPF
                     WFTAPIDANISRDELRRVVRIVAAVRKAVGDDVDVGIEFHAKFNTPAAIRIGQALEEF
                     DPYFIEEPTGSEDLDALRLVADNLRAPVASGERLVTRYAFKPMIDRHAVDILQVDPGR
                     AGGLFEARIVADMAEVALTPVIVHQPYGPIYDAMAVQLAAMIPNLLTVEWPKYYYAVE
                     PSDLRVRLVKEPLTVEQGHVHVPTKSGLGVELDEAAVQRYCIDW"
     gene            complement(2302..>3547)
                     /locus_tag="AC16G0_18895"
     CDS             complement(2302..>3547)
                     /locus_tag="AC16G0_18895"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HIR5101729.1"
                     /translation="RASTSEAKFLAKCIIGDMRHGVSEGVLLDSVARAAGAPLDTVRR
                     AAMFLGDVGEVVQRGFDGGAEALATAGPRLFHPIKPMLAQTAHTLEEAWGSAPGQLAM
                     EYKLDGARIQIHLDRDGIRLFSRRLQDVTDSLPDIVAEVRGAIASRRAILEGEVIAVD
                     ATGRIRPFQDLLQRYRRVHQVEDAQREVPLRLFLFDLLIDGEEPLFDRPYSERWQRLV
                     AAHGGLLLVPRCTPQVLAEAQAFWKEAMEAGHEGVMLKRLNSPYTPGVRGGSWLKLKR
                     ANNLDLVIVAADYGYGRRHGWLSNYHLAARDEETGEFRPVGKTFKGLTDEEFQGMTAR
                     LLAIQRREESGTVWVEPAVVVEVQYSDIQKSPLYPDGMALRFARIARIRDDKIPAEAD
                     TIQFMRRLFDHQGTRPERGREP"
BASE COUNT          604 a         1205 c         1103 g          635 t
ORIGIN      
        1 gatggaaagg ttctggaggc gtcgcgcccg ctgccccttc aggattgccc gacgcagctc
       61 ttcatccaag gcgaactcca cggggcgctc ttcaatttcc tggagcaccc ctcgtgggtc
      121 ataatacttc ggcaggtcct ctcggactcg actcggccgc cgcgccatgc ctatctcctc
      181 cgctgctgct gatagtaccg tcgttccgtc aaccgtccgt ccgggactcc gtccgttgcc
      241 aagtacgtca agttacgaaa cgtcaagctg ctgcgcgggg gattggcgag gcgatgactc
      301 tcgccaggtc ttggcgggat actccaaggg caatgagcga ccggatgagg agatcgaggg
      361 acaccgaggg gtcggccgcc tcgagcttcg ccacccggga ctggctggac cccacacgct
      421 tcgccagctc cacttgcgag agacgctttc ggtttctcag ttccctcagc ttgctgccca
      481 gcgccacctt cagctcgaca taagccgatt cttccggtgt caatcccaga aattcggccg
      541 ccgtccccac tttccaccct ttcgcttcca gtcgcttccg ctttgccttc tccatgaccg
      601 tcctctcagc acctaggagt cgtaagcccg caaccgctga atgcagatgg tgatcacgtc
      661 ctgaggcgta tgtctcgtct tcttggagaa aacttccaga atgatgattg cgtcgggatc
      721 cgcccggtat ataagccgcc aggcaacccc ctggtcggtt acccgcaact catgacacct
      781 cgatccgatt gaaggcatcg ggcgagagcg gggcaacccc aggctctcac ctcgctgcaa
      841 gcgccgcagg agcaccccca cttcaacgcg ccctgcgggc gagaacgggg gtgtcttgac
      901 ttccccatga agccagacca agggcttgtc ttcgcggctc atgcctggag agagtatgtc
      961 agatcggaca tactgtcaag cgcctattga gggagagaca acgtcagtct ctaggcccag
     1021 gccgggcttt gtggggatgt aatgggagat gccccgtaga tacggactcg aatgaactgg
     1081 gcagaggacc tcccatgacg agaaggagac tgcggcggct accagtcgat gcagtacctc
     1141 tgcaccgccg cctcgtccag ctccacgccc aggccggact tcgtagggac gtggacgtgg
     1201 ccctgctcca cggtgagcgg ctccttgacg agccggacgc ggaggtcgga cggctccacc
     1261 gcgtagtagt actttggcca ctccacggtc aggaggttgg ggatcatggc ggcgagctgg
     1321 accgccatgg cgtcgtagat cgggccgtag ggctggtgca cgatgacggg cgtcaaggcc
     1381 acctcggcca tgtcggccac gattcgggct tcgaagagcc cgcctgcccg gccgggatca
     1441 acctggagga tgtccacagc gtgccggtcg atcatgggct tgaaggcgta gcgggtgacg
     1501 agcctttcgc ccgaggccac gggcgcccgc aggttgtcgg ccacgagacg caacgcgtcc
     1561 aggtcctcgg agccggtcgg ctcctcgatg aagtaggggt cgaactcctc cagggcctgt
     1621 ccgatccgga tggccgcggg cgtgttgaac ttggcgtgga actcgatccc cacgtccacg
     1681 tcgtcgccca ccgccttgcg cacggccgcc acgattcgca ccacccgccg cagctcgtcc
     1741 cggctgatgt tggcatcgat gggcgcggtg aaccagaagg ggtcgaactt gatggccgag
     1801 aagccctcgg ccacgcgcgc ccgcgccagc tccacgtaca cctccgggcc cggctccgcc
     1861 tcgccgcccc agacctgggc gtgcaggtac gcccgcaccc tgtcccggca ctttccccct
     1921 agcaagttgt agatagaagc gcgaagcgcc ttccccacga tgtcccacag ggcgacctcg
     1981 accgctccca gggccgagac atgaagggag ctggggcccc gtacgtccag gtgcgtctta
     2041 tagacctggg accagagcga ccggatttgg aatgggtccc ggcccatcac cagcggcttc
     2101 aactcctgga tggcggcagc ctgggcccgc tcgcggaagg cgttgcccgc ctcgccatag
     2161 cccacgatgc cctcgtcggt cgtgagcttc acgaacaccc actttaggag gcccttggtg
     2221 tccaccaggt aggtttcgag gtcggtgacc ttcacggcag ccctccttta gaaacagtgg
     2281 ccggcgattg gagaagcagc ttcaaggctc tcgccctcgc tccgggcgcg tgccctggtg
     2341 gtcgaagaga cggcgcatga actggatggt gtccgcctcg gcggggatct tgtcgtcgcg
     2401 gatgcgggcg atccgggcga agcgtagcgc catgccatcc gggtacagcg ggctcttctg
     2461 gatgtcggaa tactggactt ccaccacgac cgcgggttcc acccacacgg tgccgctctc
     2521 ctcgcggcgc tggatggcca ggagcctggc ggtcatgccc tggaactctt cgtcggtcag
     2581 gcccttgaat gtcttgccaa ccggtcggaa ctcacccgtc tcctcatccc gggcggccag
     2641 gtggtaattg ctcagccagc cgtggcgccg gccgtagccg tagtctgcgg ccacgatgac
     2701 cagatccaga ttgttggccc gcttgagttt gagccagctc cccccacgca ctccgggcgt
     2761 gtacgggcta ttcagccgct tgagcatcac accttcgtgg cccgcctcca tggcctcctt
     2821 ccagaatgcc tgagcttccg ccagaacctg cggcgtgcag cgtgggacca gaagcagtcc
     2881 gccgtgcgcc gcgaccaggc gctgccagcg ttccgaatag ggccgatcga agagcggctc
     2941 ctcgccatca atcaggaggt caaacaggaa gaggcggagg gggacttctc gctgcgcatc
     3001 ttccacctgg tgcacccgcc ggtaccgctg gagcaggtcc tggaagggtc gaatgcgccc
     3061 agtagcatcc accgcaatca cctcgccctc caggatggcc cggcggctgg cgatcgcccc
     3121 ccggacctcg gccacgatgt ccggaaggct atcggtcaca tcctgcaggc ggcgagagaa
     3181 cagccggatc ccgtcgcggt ccaggtgaat ctggatgcgt gccccgtcca gcttgtattc
     3241 catggcgagc tgaccgggcg cgctgcccca ggcttcctcc agcgtgtgcg ccgtctgcgc
     3301 cagcatcggc ttgatgggat ggaatagcct ggggccagca gtggcaagcg cctctgcccc
     3361 cccgtcaaag ccgcgctgga ccacctcgcc gacatccccc aggaacatgg ccgcccgccg
     3421 gactgtgtcc aagggcgcac cggctgcacg cgccacgctg tccaagagga ccccctcgct
     3481 cacgccgtgc cgcatgtcgc cgatgatgca tttggcgagg aacttggcct cggaggtgga
     3541 tgcgcgc
//
LOCUS       DBNGZC010001096         7459 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1271147, whole genome shotgun sequence.
ACCESSION   DBNGZC010001096 DBNGZC010000000
VERSION     DBNGZC010001096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 7459)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7459)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7459
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1271147"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(442..1233)
                     /gene="uppS"
                     /locus_tag="AC16G0_18900"
     CDS             complement(442..1233)
                     /gene="uppS"
                     /locus_tag="AC16G0_18900"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013640988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HIR5101730.1"
                     /translation="MGLWKRLWKLVYHIYQRNLLAEIGRHELPQHVGLILDGNRRYAR
                     SVGLPDLLEGHRLGAKKLEEVLTWCEELNIRMVSIWILSTENLQRSPEELSGLLSLIE
                     NKLRDAARDPKTHKNRMRIRAIGKLELLPPSMQEAIREAEEATREYNGFFLNVAVGYG
                     GRQEIADAVQSLLRDRTAQGMSLQAAIYEVTPDNVGKYLYTYDLPDPDLIIRTSGEVR
                     LSGFLLWQSAYSEYHFCDAYWPAFRRIDLLRAIRSYQQRERRFGF"
     gene            complement(1235..2230)
                     /locus_tag="AC16G0_18905"
     CDS             complement(1235..2230)
                     /locus_tag="AC16G0_18905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101731.1"
                     /translation="MWAVFVLIGWMAWGELSAPGYALAAGGTGALRSGTFDRTALQNE
                     LSRSLSELRAWSDEQLRTYGDRLAQLQASLEGESGLSPSERDDLNQRLLTRAAQVGLL
                     LRERARAPAAMEGKRGASAVESGPVWLVGLAGWFTTESGLLLGLVGGLVIAFALGNLF
                     GYRRGARQASYYGEADPRFRFLRRPAAPSARRKEPGRITLAMIRGALSDGRTVLLQLG
                     YDIAPSRRSHYLDLVRQMQEALDGAQGLTHSAWEDPRHPHRFYELLVCQRLEALDTLT
                     VTEGLLAKLGEEIEACRISGGLILRRVWWSVPAGSESARSHLPIPGAASIRGGVS"
     gene            complement(2392..2694)
                     /locus_tag="AC16G0_18910"
     CDS             complement(2392..2694)
                     /locus_tag="AC16G0_18910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HIR5101732.1"
                     /translation="MDTLAVILGMAIATYATRAPALLLLSRRLPPGLERFLSHIPAAI
                     FAALLAPPLLVPGGTFNPGADAAAAIPAAIAAWWTRRVLPTILAGLLAYWLIRWLG"
     gene            complement(2676..3395)
                     /locus_tag="AC16G0_18915"
     CDS             complement(2676..3395)
                     /locus_tag="AC16G0_18915"
                     /inference="COORDINATES: protein motif:HMM:NF015549.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIR5101733.1"
                     /translation="MSQPPLSPSAGSPSRRLREGLWATLPLIPGVAVFGVVYAVLARQ
                     AGLNLWATLGMSALVFAGASQFIAVSMWGQAGGGAIILTTCIINLRHLLMGASLAPYL
                     RGERRAWKALLAFAMADESYALAVSRYLRGDGSREFFLGANLGVYAGWVLGSLAGGLV
                     GGLVVDPGRWGLGLVFPLTFLGLLVPLLTRWVTAAVALISGIVAVTTAAWLPGKTHLF
                     LAILLASGIGMLLERWWTPSR"
     gene            complement(3392..4081)
                     /gene="nth"
                     /locus_tag="AC16G0_18920"
     CDS             complement(3392..4081)
                     /gene="nth"
                     /locus_tag="AC16G0_18920"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011290565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR5101734.1"
                     /translation="MARTVRAATKTGGRGSAPAGPTKQQVGKILTVLGRTYPDARCEL
                     NFTTPLELLVATILSAQCTDARVNLVTPQLFKKYRSSKDFANADPAILEGEIRTTGFF
                     RSKAKNIIACCQTLVRDFRGVVPRSMEELVTLPGVGRKTANIVLYNAYGIPGFGVDTH
                     VVRVTNRLGLVDTEDAEKIEEAVCRVIPREEWGQATHLFIFHGRRTCNAKQPLCPQCT
                     VRALCPWPGKR"
     gene            complement(4081..4464)
                     /locus_tag="AC16G0_18925"
     CDS             complement(4081..4464)
                     /locus_tag="AC16G0_18925"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5101735.1"
                     /translation="MSWQYDHTHFKCSDHEKTVAFFKENFGAREAKRWEVNGMAIVTL
                     DIGGLMYNFSPKRANEIPDTTTGKVHYGVYHIALKTNDLVSEVARMKSRGVKITQEVG
                     QANPTTKFAFVEGPDGISIEVLQRD"
     gene            complement(4518..5957)
                     /locus_tag="AC16G0_18930"
     CDS             complement(4518..5957)
                     /locus_tag="AC16G0_18930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025951261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HIR5101736.1"
                     /translation="MASRPSASYSITMRVEIQNRPGMLGRVTSAIGKAGGDIGAVDIA
                     GFGKDTIVRDITVNARDEAHEKVIAVAVGKLPGVKVVNVSDRTFLVHLGGKITVNAKR
                     PIKTRTDLSMVYTPGVARVCMAIHEDPKKAYQLTIKKNCVAVITDGSAVLGLGNIGPA
                     AAMPVMEGKAALFKEFAGVDAFPICLDTQDPDEIVRTAKLIAPGFGGINLEDISAPRC
                     FEIEERLQKELDIPVFHDDQHGTAVVVLAALLNALRIVKKKLKDLKIVVSGVGASGVA
                     CTKVMMAMGARNIIGLDTKGVLHRDRKEHMNAVKTWYAKVTNPQNLKGGILDVMRGAD
                     MYLGLSGPGSLPLKAVKRMARDPIIFAMANPVPEIMPEEAAPYVRVMATGRSDYPNQI
                     NNVCCFPGFFRGLLDSQSPCVNQAMKIGAAKAIASLVSARELNEEYITPSVFDTRVAP
                     AVAEAVARAAHQTGVARRARKAMGGYRPL"
     gene            complement(6016..7155)
                     /locus_tag="AC16G0_18935"
     CDS             complement(6016..7155)
                     /locus_tag="AC16G0_18935"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012175004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR5101737.1"
                     /translation="MIATRAQEIEPFVVMEVLERAQELEARGEHLIHLEVGEPDFPTP
                     SCIQEALAKAVKDGMTHYTHSLGIPPLREAIAEHYHQKYGVEVSPDRILVTAGTSPAM
                     WMVFSAILEPGDEVLVSDPHYACYPNFIRFCGGELACVDILEESGFQLRPEAVKARIT
                     PRTKALLINSPANPTGKLLSAERMAALANLGPMVLSDEIYHGLVYEGREHTILEFTDR
                     AVVFNGFSKAFAMTGWRLGYVIVPPELIRPLQKMHQNFFISANAFVQAAGIAALREAL
                     PDSERMRQSYDERRRYLVPGLKKLGFGIATEPNGAFYVFANAKAFSGDSYSFAFEILE
                     RAKVSLAPGVGFGKNGEGYLRFSYANSLENLGEGLRRLEVFLAQR"
BASE COUNT         1326 a         2506 c         2370 g         1257 t
ORIGIN      
        1 ccgcccgaca tcaaggttat ctaccaacgc tggttcaagg gattctcgta agcgggaggg
       61 aatctcggct gcagcctgcg gtccccgaat gatcatcctc gccttggctg gatgccatgg
      121 aaccgagacc atcccagggg gacacgatcc tctgccccgg ctgcggcggc cgcggacagt
      181 tcgagtgggc cccgagtacg aaggtcccga agacctgggc cccgagcgcc atctgcgcca
      241 tgtgtcaggg gactgggcgc gtccccgcgt cctggatgag cggccgaaac aaatgaccaa
      301 ttccaaaatc ccaatggagg cccgagcctg gtccgcagtc tcagcctgcg ttacagttgt
      361 cggtaccacg tttggtcatt ggtcaattga gatttggtca ttacccggat tctccacgaa
      421 cgtgatcgcg gataatccgg gtcagaaccc gaagcggcgc tcgcgttgct ggtaacttcg
      481 gatggcgcgc aggagatcga tgcgccggaa ggccggccaa taggcgtcgc agaaatggta
      541 ctcgctgtat gcactctgcc agagcaggaa gccgctcaat cgcacctccc cgctggtccg
      601 gatgatgagg tcgggatcgg gcaggtcgta ggtgtaaagg tacttcccca cattgtctgg
      661 ggtgacctcg tagatcgccg cctggagcga catcccctgc gccgtccggt cgcgcaagag
      721 gctctgcacc gcatccgcaa tctcctggcg cccgccgtat cccactgcca cgttcaggaa
      781 gaagccgttg tactcccggg tggcctcctc cgcttcgcgg atcgcctcct gcatcgaggg
      841 aggcagcaat tcaagtttgc cgatggcccg aattcgcatc cggttcttgt gagtcttggg
      901 gtcccgcgcc gcatcgcgca gcttgttctc gatgagggag aggagacccg aaagctcctc
      961 gggcgatcgc tgcaggttct ccgtggacag gatccagatg ctcaccatcc ggatgttcag
     1021 ttcctcgcac caggtgagca cctcctccaa cttcttggcc cccaggcgat gcccttcgag
     1081 cagatcgggt aatccgacgg atcgcgcgta gcgccgattg ccgtccagga tgagacccac
     1141 gtgctggggc agctcgtgcc ggccgatctc cgccaggaga tttcgttgat agatgtggta
     1201 cacgagcttc cacagcctct tccacaatcc cacgtcagga cactcccccg cggatcgacg
     1261 cggcgcccgg aatcggtagg tgggagcgtg cgctctctga tcccgcggga acgctccacc
     1321 agacgcgccg caggatgagc ccaccggata tacgacacgc ctcgatctct tcgcccagct
     1381 ttgccaggag gccttcggtg acagtcaggg tatccagggc ctccagccgc tgacacacca
     1441 gcaactcgta gaatcggtga ggatgccggg gatcctccca ggcggagtgc gtcagccctt
     1501 gcgcgccgtc caaggcttct tgcatctgcc gcaccaaatc caggtagtgg gaccggcgcg
     1561 acggcgcgat gtcgtatccc agttgcagca ggaccgttcg cccgtcgctc agcgccccgc
     1621 ggatcatggc caaggtgatc cgtccgggct ccttccggcg cgccgatggc gcggccggcc
     1681 gcctcaggaa tcggaaccgc gggtccgcct cgccgtaata cgaggcttgg cgcgcgcccc
     1741 ggcgatagcc gaagaggttc ccgagggcaa aggcaatcac caggccaccc acaagcccca
     1801 ggaggaggcc cgattccgtc gtgaaccagc cggccagacc caccagccac accggtccgg
     1861 attcgacggc tgaagccccg cgcttgccct ccatcgccgc aggtgccctg gcacgctccc
     1921 gcagcagcag acccacctgg gccgcgcggg tcaggaggcg ttggttcagg tcgtctcgct
     1981 ccgatggcga cagacccgat tccccttcca gggaggcctg gagctgcgcc agccggtccc
     2041 cgtacgtccg gagctgctca tccgaccacg cccgtaactc cgatagcgag cgggataact
     2101 cgttctgaag cgccgtccgg tcgaacgtgc cggagcgaag ggcccccgtg ccccccgcgg
     2161 cgagcgcgta gccaggagcg gataactcac cccatgccat ccagccgatc agcacgaaaa
     2221 ccgcccacat ctcaggacgc catggcttca cacggatcag gggtaggatg gatggatatg
     2281 aacttcgctc aggcgaggga cgcgaaagac catacttgag gatgcacggc attgcaactg
     2341 aataaaccgg ctccgaggcg acgcgcaacc cggacccatc caattgctcg atcatcccag
     2401 ccatcggatc agccagtagg ccagcagacc cgccaggatc gtcgggagaa cccgacgcgt
     2461 ccaccacgcc gcaatggcgg cagggatcgc ggccgcggca tccgctcccg gattgaaagt
     2521 ccccccgggc accagaagcg gaggcgccag gagggcggcg aaaatcgcgg ccgggatgtg
     2581 gctcaggaat cgctcgagac ccggaggcaa ccgccgggac agcagcaaga gtgcgggggc
     2641 ccgcgtcgcg taggtggcga tcgccattcc caggatcacc gcgagggtgt ccaccacctc
     2701 tccaggagca tgccgattcc gctcgccagc agaatcgcca ggaacaggtg cgtcttgccg
     2761 ggcaaccagg cggctgtggt caccgccacg atccccgaaa tgagggcgac cgccgccgtc
     2821 acccagcggg tcaagagcgg cacgaggagt cccaggaagg tcaggggaaa tacgagcccg
     2881 agtccccaac ggccggggtc caccaccagc ccgcccacca ggccacctgc caggctgccg
     2941 agtacccagc cggcgtagac gcccaggttc gcccccagga aaaactcgcg gctcccgtca
     3001 ccccgcaggt aacgggagac agccagggca taggattcat ccgccatcgc gaaggccagg
     3061 agcgccttcc atgccctgcg ctctccccgc aggtacgggg ccagcgaggc ccccatcagg
     3121 aggtggcgga gattgatgat gcaggtggtg aggatgatgg cgccgccgcc ggcctgcccc
     3181 cacatgctga cggcgatgaa ctgggacgct cccgcaaaga ccagggcgga catccccagg
     3241 gtagcccaga gattcagccc ggcctgccgc gccaggacgg catacaccac gccgaagacc
     3301 gctaccccgg ggatcagggg aagggtggcc cacaggcctt ctctcaggcg ccgcgagggg
     3361 gaaccggcgg acggcgacag tgggggctgg ctcatcgctt cccgggccag gggcagaggg
     3421 cgcgcaccgt acactggggg cacaggggtt gcttcgcgtt gcacgtgcgg cggccgtgaa
     3481 agatgaagag gtgggtcgcc tgcccccact cctcccgggg gatcacccgg cacactgcct
     3541 cctcgatctt ctccgcgtcc tccgtgtcca ccagacccag gcgattggtg acgcggacca
     3601 cgtgggtgtc caccccgaat cctggaatcc cgtaggcatt gtacaggacg atgttggcgg
     3661 tcttccggcc cacgcccggg agggtgacca gctcttccat gctccgtggc accacccccc
     3721 ggaaatcccg gaccagcgtc tgacagcagg cgatgatgtt cttggccttg cttcggaaga
     3781 agccggtggt gcggatctcc ccctcgagga tggcgggatc cgcgttggcg aaatccttgg
     3841 acgatcggta cttcttgaag agctgaggtg tgaccaggtt gacccgggcg tcggtacact
     3901 gggcggagag gatggtggcc accagaagct ccagcggcgt cgtgaagttg agctcgcagc
     3961 gggcatccgg ataggtacgc cccaggacgg tcaggatctt tccgacctgc tgcttcgtgg
     4021 ggccggcggg cgcgctgccg cgcccgccgg tcttcgtcgc tgcccgcacc gtgcgcgcca
     4081 tcagtctcgc tgcagaacct cgatggagat cccatccggc ccctcgacga acgcgaactt
     4141 ggttgtcggg ttcgcctggc cgacctcctg ggtgatcttc acaccgcgac tcttcatccg
     4201 tgccacctcg gagaccaggt cattcgtctt gagggcgatg tgatacacgc cgtagtgcac
     4261 cttccccgtc gtcgtgtccg ggatctcgtt cgctcgtttg ggggagaagt tgtacatgag
     4321 ccccccgatg tccagggtca cgatggccat cccgttcacc tcccaccgct ttgcctccct
     4381 ggcgccgaag ttctccttga agaaagccac ggtcttctca tggtcgctgc acttgaagtg
     4441 ggtatgatcg tactgccagc tcatcctggt cctccttgag ggacagcggg ttctggatcc
     4501 ccctccacac ggccgggcta caacggtcgg tagcccccca tcgccttgcg ggctcggcgt
     4561 gcgacaccgg tttggtgcgc ggcgcgcgcc acagcctcgg ccacggctgg cgcgacccgc
     4621 gtgtcgaaga cgctgggggt gatgtactcc tcgttcagct cccgagcgct caccagcgag
     4681 gcgatggcct tggccgcgcc gatcttcatg gcctggttca cgcaggggga ttgcgagtcc
     4741 agcaggcctc ggaagaatcc cgggaagcag cagacgttgt tgatctggtt cgggtaatcc
     4801 gaccgccccg tggccatcac ccggacgtag ggggcggcct cctccggcat gatctcggga
     4861 accggattcg ccatggcaaa gatgatggga tcgcgggcca tccgcttcac ggccttgagg
     4921 ggcagcgacc ccggccccga gaggcccagg tacatatcgg cgccccgcat cacgtccagg
     4981 ataccgccct tcaggttctg cgggttggtg accttggcat accaggtctt gaccgcattc
     5041 atgtgctcct tgcggtcgcg atgcaggacg cctttcgtgt ccaggccgat gatattgcgg
     5101 gcgcccatgg ccatcatgac cttggtgcag gccacgccgg acgcgccgac gccgctcacc
     5161 acgatcttca ggtccttgag cttcttcttg acgatccgca gggcgttgag cagggcggcc
     5221 aggaccacca cggccgtccc gtgctgatcg tcgtgaaaca ccgggatgtc cagctccttc
     5281 tgcagacgct cctcgatctc gaagcagcgg ggggcggaga tatcttccag gttgatgccc
     5341 ccgaagcccg gggcgatgag cttcgccgtc cgcacgatct cgtccgggtc ctgggtgtcg
     5401 agacagatgg ggaaggcgtc cacgcccgca aactccttga acagggccgc cttgccctcc
     5461 atgaccggca tggccgccgc cggaccgatg ttgcccagac ccaggacggc ggagccatcg
     5521 gtgatcaccg ccacgcagtt cttcttgatg gtgagctggt aggccttctt ggggtcctcg
     5581 tggatcgcca tgcagacccg cgccaccccc ggcgtataga ccatggagag atcggtccgg
     5641 gtcttgatcg gtcgcttggc gttcaccgtg attttccccc ccaggtggac gaggaaggtc
     5701 cgatccgaga cgttcaccac cttgacaccc gggagcttcc ccacggcgac ggcgatgacc
     5761 ttctcgtggg cctcatccct ggcgttcacg gtgatgtccc ggacgatggt gtccttgccg
     5821 aagccggcga tgtccacggc gccgatgtcg ccccccgcct tgccgatggc agaggtgacc
     5881 cgtcccagca ttcccgggcg gttctggatc tccacgcgca tggtgatgct gtagctggca
     5941 ctcggtcgtg aagccatgat gcctcccccc tccgatgcgt tggttgatcg ggatgagcgc
     6001 gccgcggcct ccccactacc gctgcgccaa gaagacctcc aaccggcgga gcccctcgcc
     6061 caggttctcc agcgagttgg cgtaggagaa ccgcaggtag ccctcgccgt tcttgccgaa
     6121 acctacaccg ggagccaggc tgaccttggc gcgctccagg atctcgaagg cgaagctgta
     6181 cgaatccccg ctgaaggcct tggcgttggc aaagacgtag aaggccccgt tcggctccgt
     6241 ggcgatgccg aagcccagtt tcttgaggcc gggaacgagg taccgccgcc gctcgtcgta
     6301 actctggcgc atgcgttccg agtccggcag cgcctctcgc aacgcggcga tcccggcggc
     6361 ctgcacaaag gcattggcgg agatgaaaaa gttctggtgc atcttctgga gcgggcggat
     6421 cagttcgggc ggcacgatga cgtagcccag ccgccacccg gtcatggcga acgccttgga
     6481 gaagccgttg aagaccacgg cgcgatccgt gaactccagg atcgtatgct cccgcccctc
     6541 gtacaccagg ccgtggtaaa tctcgtcgga gagcaccatc ggccccaggt tcgccagggc
     6601 ggccatccgt tccgccgaga ggagcttccc cgtgggattg gccggagaat tgatgagaag
     6661 ggccttggtc cgcggggtga tccgcgcctt cacggcctcc ggccggagct ggaacccgct
     6721 ctcttccaga atgtccacgc acgccagctc accgccgcag aaccggatga aattggggta
     6781 gcaggcgtag tgcgggtcgg agacgaggac ctcgtcaccc ggctccagga tggcggagaa
     6841 gaccatccac atggcgggcg aggtccccgc cgtcaccaga atgcgatcgg gagacacctc
     6901 cacgccatac ttctggtgat agtgctccgc gatcgcctcc cgcaaggggg ggatgcccag
     6961 gctgtgggtg tagtgggtca tgccatcctt gacagccttg gccaaagctt cctggatgca
     7021 ggacggggtg ggaaaatccg gctcgcccac ctccaggtgg atgaggtgct ctcccctggc
     7081 ctccagctcc tgggcccgct ccaggacctc catcaccaca aagggctcga tctcctgggc
     7141 tcgcgttgcg atcaccggga acctcctcac acgcagaata agagatgaca ctggcccgca
     7201 gcagggttac ggcggacgac ccgaggcggg gggatgataa cacagccatc ccgcgaagca
     7261 aaccctttgg ccacgaggac gaacaatcca acggttgaag gcgaaccgct ggatgctcag
     7321 agactggtga gcacgtgcat cgtcgccgcc gatccccatt agtggtcggg aagccgactc
     7381 ctggatggcc ctccgggtct actcctcggc gaggcgaaag gccgtctcgg ccaagacggc
     7441 cgctcccgcg gcggcatcc
//
LOCUS       DBNGZC010001097         3606 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1070056, whole genome shotgun sequence.
ACCESSION   DBNGZC010001097 DBNGZC010000000
VERSION     DBNGZC010001097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1070056"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(363..866)
                     /locus_tag="AC16G0_18940"
     CDS             complement(363..866)
                     /locus_tag="AC16G0_18940"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5101738.1"
                     /translation="MNRQDILTMLEYTAWANERVLEAAAALSTEAFLRDMGNSFPSVR
                     DTLAHILGAEWVWLRRWQGESPTTFAGAGELTTLPRLKDRYAAVERERRAFVEALPEA
                     RLAEPFTYRDMAGNSHSLLLVQSLQHVVNHGTYHRGQVTTLIRQLGAKPVSTDLSRFY
                     LDRAAGA"
     gene            complement(879..1559)
                     /locus_tag="AC16G0_18945"
     CDS             complement(879..1559)
                     /locus_tag="AC16G0_18945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HIR5101739.1"
                     /translation="MTEYSGPTCALCGVRACTAEPDTKTPPPFCPMPAEEELLQEVER
                     QYLEREDLRRLAVESARTEAAGYCTRTRVEEIMDFARRIGAQRLGIAHCVGLMQEAKV
                     ARDIFVAGGFDVYTVCCKAGSIAKEKVGLRDDEKVHPGQYEAMCSPVGQAALLHSAGT
                     QLNVVIGLCVGHDSLFFMHSKAPATVLVAKDRVLGHNPVAALHTTHSYYRRLTQPREG
                     SQTPHGGK"
     gene            complement(1609..2034)
                     /locus_tag="AC16G0_18950"
     CDS             complement(1609..2034)
                     /locus_tag="AC16G0_18950"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5101740.1"
                     /translation="MRKPMETLLAETPVKAALDRMQTLGLSSLPVQNQAGSFLGIVRA
                     ADLSCRVASAESEGVAPIKNCLASSAVTDTPIALLWLMSQGQDIVPIPETKRRSYVEE
                     NLSAQAIKLTAGYLRRIHEIAPKGATAGDRYADMRSINR"
     gene            complement(2186..>3606)
                     /locus_tag="AC16G0_18955"
     CDS             complement(2186..>3606)
                     /locus_tag="AC16G0_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR5101741.1"
                     /translation="GWRKRLAIARELVKAPDVLLMDEPTNHLDVEGILWLEDLLTAGS
                     LAYLVVSHDRYFLENVARRMLELNRCYAGGFFETEGRYSDFLVKREEAIREQATYQDT
                     LANKVRREVEWLRRGAKARTRKAQARIKQAGRLIQELQEVTARSKTAKAGIDFTASER
                     RSKKLLVARDLTKAFGPRRIVSGLNLTLAPGMRLGLLGPNGSGKSTLLKLLAGTLAPD
                     RGQIERAEGLRIVAFDQYRAGLETAASLRRALAPEGDAVVYRGQSIHVVSWAKRFLFR
                     PEQLEIPVGRLSGGEQARILIATLMREPADLLILDEPTNDLDIPTLEVLEESLADFPG
                     GLVLVTRDRFMLERVSTLILALDGAGGALFFGDYAQWEGAQEAESAAARVAVAPTKVP
                     PREAGGPKRLTYLEKREWEQMEQAILAAEARLVVCRQALEDPGVASDPVALQQRYEAL
                     EAARAEVERLFARWAELESRQP"
BASE COUNT          578 a         1257 c         1125 g          646 t
ORIGIN      
        1 gagacattcg cgggcgtagg aggaacaaac caaagtcgga gcgcgccccg cgggcatcgt
       61 ggtaccgcca tgcgagatta ccagaatgcc tcacgcgcca caacgcttca cgcacaagga
      121 ccggcgaggg ggagctatcg gggagtagag ggatgctgcc agcgaggcgc cttgggcccc
      181 tcactcgtcc gccgaagttg cgggcagact ctgggcggac tgatcagggt atacgacgcg
      241 gccttccgga gggtcgggtg ggttgatgat ggaagaagcc ttgtggcgag aggtcccgag
      301 ccctcctgct gcccggatcc gcccaggccg gcaggcggcc atccaatgca gcacacggcg
      361 cctcaggcac cggccgctcg gtccagatag aaacgactca ggtccgtgga gacaggcttg
      421 gcgccaagct gccggataag cgtcgtgacc tggcctcgat ggtaggtccc atgatttacg
      481 acatgctgga gactctggac cagcaggagg ctgtgcgagt ttcccgccat gtcccggtag
      541 gtgaacggct cggccaggcg cgcttcgggc agggcttcca cgaaggcacg gcgctcgcgt
      601 tcgacggcgg cataccgatc tttcaagcgt ggcagcgtgg taagctcgcc cgccccggcg
      661 aaggtcgttg gagactcccc ctgccatcgg cgcagccaga cccactcggc gcccagtatg
      721 tgggctaagg tgtcccgcac cgaagggaag ctgttcccca tgtcccggag gaaggcctcg
      781 gtcgaaagtg ctgcggcggc ctcgagcacc cgttcgttgg cccaagcggt gtactccagc
      841 atggtgagaa tgtcctgtcg attcatgggc ggctccttct attttccccc atggggagtc
      901 tgcgacccct ccctcggctg cgtcaacctg cggtaatagg aatgggtcgt gtgaagagcg
      961 gcgaccgggt tatgccccag gacccgatcc ttggccacca ggaccgtggc tggggctttg
     1021 gagtgcatga agaagaggct gtcgtgccca acgcacagcc cgatcaccac gttgagctgg
     1081 gtcccggccg aatggagcag ggcggcttgc ccgaccggac tgcacatggc ctcgtactgg
     1141 cctgggtgta ccttctcgtc gtcccgcagg ccgacctttt ccttggcgat ggaccctgcc
     1201 ttgcagcaga cggtgtagac gtcaaacccg cccgccacga aaatgtccct cgccaccttg
     1261 gcctcctgca tgaggccgac gcaatgcgcg atccccagcc gttgcgcgcc gatgcgccgg
     1321 gcgaagtcca tgatttcttc gacgcgggtc ctcgtgcagt agcccgcggc ctccgtccgc
     1381 gccgactcca ccgccagtct ccgcagatcc tcccgctcca ggtactgccg ctcgacctcc
     1441 tgcagcaact cctcttccgc cggcatcggg cagaagggcg gcggcgtctt cgtgtccggc
     1501 tcggcggtgc aggccctcac cccgcagagc gcgcacgtgg gtccgctgta ttccgtcatg
     1561 atgactctgt cctcctcgtg ggcgcgcccc aatcgggcgg cagtggactc agcggttgat
     1621 gctgcgcatg tcggcatacc ggtcgccggc agtggcgccc ttcggggcga tctcatggat
     1681 gcgccgcaga tacccggcgg tcaacttgat cgcctgcgcc gagaggttct cctcgacata
     1741 gctgcgccgc ttggtctcgg gaatcggcac aatgtcctgt ccctgggaca taagccagag
     1801 cagggcaatg ggtgtgtccg tcaccgccga ggaggcaaga caattcttga tcggagccac
     1861 cccttcgctc tcggcgctcg cgactcgaca cgaaaggtcg gccgccctca cgatcccgag
     1921 aaacgaccct gcctgattct gaacgggcaa ggaactcagc cccagcgtct gcatccgatc
     1981 cagggccgcc ttgaccggtg tctccgccag cagggtctcc atcggctttc gcatgctgtc
     2041 cctgattttt ttcctgcctc ctccccaggg taaaccgtca ctcttccaga gcctcggcgt
     2101 ccctttcgag tcattctcac acagtagccg cgcccggaca atcctccgcc ccatgcccat
     2161 cccggtcccg acggcagagg cctcgttacg gttgccggct ctccagctcc gcccagcggg
     2221 cgaacagtcg ctccacttcg gcgcgagccg cctccagcgc ctcgtagcgc tgctgcaggg
     2281 ccacaggatc cgaggccacc ccggggtcct ccagcgcttg ccggcaaacc acgagacgtg
     2341 cttccgccgc caggatcgcc tgctccatct gctcccattc gcgcttttcg agataggtca
     2401 ggcgctttgg accaccggct tcccggggcg gtaccttggt cggggcaaca gccacccgcg
     2461 cggccgcaga ctccgcctcc tgcgccccct cccattgggc gtagtcgccg aagaacagcg
     2521 ctcccccggc accgtccaga gccaggatga gtgtcgagac gcgctccagc atgaagcggt
     2581 cgcgcgtgac cagcaccagt cctcccggaa agtcggcgag actctcttcc aggacttcga
     2641 gcgtcgggat gtccaggtca ttcgtcggct cgtcgaggat cagcagatcg gccggctccc
     2701 gcatcagcgt ggcgatcagg atccgcgcct gctcaccccc cgagagccgt cctaccggga
     2761 tctccagttg ctctgggcgg aagaggaaac gtttggccca ggagactacg tggatggatt
     2821 gtcctcgata caccaccgcg tccccctccg gggcgagggc gcgtcggagg gaggcagccg
     2881 tctcgagtcc cgcgcgatac tgatcgaagg cgacgattcg caagccttcc gcgcgttcaa
     2941 tctggccgcg gtccggcgcc agggtgccgg cgagcagctt caggagcgtg ctcttgccac
     3001 tgccgttcgg ccccagcaag ccgagccgca tgccgggagc cagggtcagg ttcaggccgg
     3061 agacgatccg ccgcgggccg aaggccttgg tgagatctcg ggcgaccagg agcttcttcg
     3121 acctgcgctc cgaggcggtg aagtcgatcc cggccttggc ggtcttgctc ctggccgtga
     3181 cctcctgcag ttcctggatc agccgccccg cttgcttgat ccgggcctgg gccttccgcg
     3241 tgcgggcctt ggcccctcgc cgaagccact ccacttctcg ccggaccttg ttcgccagcg
     3301 tgtcctgata ggtcgcctgc tctcggatgg cctcctccct cttcaccagg aagtcgctgt
     3361 accgcccttc tgtctcgaag aaccccccgg cgtagcaccg gttcagctcc agcatccgcc
     3421 tggcgacgtt ctccaggaag tagcggtcgt ggctgacgac caggtacgcc aggctcccgg
     3481 ccgtgagcag atcctcgagc cacagaatcc cttcgacatc cagatggttt gtcggctcat
     3541 ccatcagcag gacgtcgggg gccttcacca gctcgcgggc aatggccagc cgcttccgcc
     3601 atcctc
//
LOCUS       DBNGZC010001098         3016 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_193945, whole genome shotgun sequence.
ACCESSION   DBNGZC010001098 DBNGZC010000000
VERSION     DBNGZC010001098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_193945"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="AC16G0_18960"
     CDS             complement(<1..644)
                     /locus_tag="AC16G0_18960"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR5101742.1"
                     /translation="MANTGKIVQVIGPVVDVEFDPGKLPAIYNALEVSGRKVEDIFSY
                     SERLVLEVAQHLGESHVRTVAMSSTDGLTRGMEVKDTGSPIMIPVGPNTLGRIINIIG
                     EPVDKLGPITSDTMYPIHRPAPGFEEQETKVQMLETGIKVVDLLEPYTKGGKTGLFGG
                     AGVGKTVIIMELIRNIAQEHGGISVFSGVGERTREGNDLWLEMKESGVINKTALV"
     gene            complement(810..1706)
                     /gene="atpG"
                     /locus_tag="AC16G0_18965"
     CDS             complement(810..1706)
                     /gene="atpG"
                     /locus_tag="AC16G0_18965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HIR5101743.1"
                     /translation="MATLRDIRRRITSVKSTQQITRAMKLVAAAKLRRAQERILEARP
                     YSHKMGEVLAWLALRSPRGLHPLLERRTSEENGTSRKLLLVVSGDRGLCGAFNSNIIR
                     RSVEFLRTYQNDPEISLVLIVVGRKVRDFYRRRGQYAVRSEYTNFFDKLAYAHAAQIG
                     QDVIRGYVGEEVDEVHLIYNEFKSVATQRVVVEQLLPIQPQEVPADAPVVEFQFEPSP
                     AAVLERLLPKHVEVQIYRALMESLAAEYGARMTAMENATKNASEMIDLLTLQFNKARQ
                     ERITKELLEIVGGAEALRQSEG"
     gene            complement(1776..>3016)
                     /gene="atpA"
                     /locus_tag="AC16G0_18970"
     CDS             complement(1776..>3016)
                     /gene="atpA"
                     /locus_tag="AC16G0_18970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR5101744.1"
                     /translation="SVPVGEAMIGRVVNALGQPIDDKGPIITTQFNPIERLAPGVVDR
                     RPVKEPLQTGIKAIDAMIPIGRGQRELIIGDRQTGKTAIAVDTIINQKGKDVYCFYVA
                     TGQKRSTVAQVVKVLEEAGAMAYTTVVVASASEPAPLQYISPYTGCTMGEYFRDTKRH
                     ALCIYDDLTKHAAAYRQLSLLLRRPPGREAYPGDVFYLHSRLLERAAKLNDQLGGGSL
                     TSLPIIETQLGDVSAYIPTNVISITDGQIYLETDLFYAGIRPAVNVGLSVSRVGGAAQ
                     IKAMKQVAGKLRLDLAQYREMAAFAQFGSELDKATQAQLNRGQRMVEVLKQGQYRPVP
                     VERQVLIIHAGTSGHLDDLPAESVASFEETLYKFAEMRHPEIYRELVEKKEIGDALRG
                     RMDECIQACKAEFLATAKAA"
BASE COUNT          475 a         1012 c          953 g          576 t
ORIGIN      
        1 acgagggcgg tcttgttgat gacccccgac tccttcatct ccagccagag gtcgttcccc
       61 tcgcgcgtcc gctcgcccac gcccgagaag accgagatgc cgccgtgctc ctgggcgatg
      121 ttgcggatga gctccatgat gatgacggtt ttccccacgc ccgccccgcc gaagagtccg
      181 gtcttgcccc ccttggtgta gggctccagc aggtccacga ccttgatgcc cgtctccagc
      241 atctggacct tggtctcctg ctcctcgaag ccgggtgccg gccggtggat ggggtacatg
      301 gtgtccgagg tgatcggccc cagcttgtcc actggctccc cgatgatatt tatgatcctg
      361 cctagggtgt tcgggccgac cgggatcatg atcgggctac cggtgtcctt gacctccatt
      421 ccccgcgtca aaccgtccgt ggaggacatg gccaccgtcc ggacatggct ctcgcccagg
      481 tgctgggcca cctccagcac gagccgttca ctgtacgaga agatatcctc caccttccga
      541 ccgctgacct ccagggcgtt atagatggcc gggagtttcc cggggtcgaa ctccacgtcc
      601 acgaccggtc caatgacctg cacgatcttc cccgtgttcg ccatcttgcg ctcccttgac
      661 gcgcgcccag gcgcgcgcca tccctattgc gtcgtggctt gtagcccttc aagcctcaga
      721 gagttgcggt agtcccagtc gtccgccgcc cccgtgtcgg ccgagtggca ggggaacgat
      781 ccagcaatcg aacgcctctt tccgccacgt catccctccg actgccgcaa ggcctcggcg
      841 ccgcccacga tctccagcag ttccttggtg atccgctcct ggcgtgcctt gttgaactgc
      901 agggtgagga ggtcgatcat ctccgaggcg ttcttggtgg cgttctccat ggcggtcatg
      961 cgcgcaccgt actccgccgc cagggactcc atgagtgcgc ggtagatctg cacctccacg
     1021 tgcttgggta agaggcgctc cagaaccgcg gccggcgacg gctcgaactg aaactccacc
     1081 accggggcgt ccgcgggaac ttcctgcggc tggatgggca ggagctgctc caccaccacg
     1141 cgctgcgtgg cgacggattt gaactcgttg tagatgaggt gcacctcgtc cacctcttct
     1201 ccgacatagc cccggatcac gtcctggccg atctgggcgg cgtgtgcgta ggcgagttta
     1261 tcgaagaagt tggtgtactc ggagcgcaca gcgtactggc cgcgtcgccg gtagaagtcc
     1321 cggaccttcc gcccgaccac gatcaggacc aggctgatct cggggtcgtt ctgataggtc
     1381 cggaggaact ccaccgaccg ccggatgatg ttgctgttga acgcgccaca gaggccccgg
     1441 tccccgctga ccaccaagag cagcttccgg ctggtgccgt tctcctccga cgtccgccgc
     1501 tccaaaagag gatggagccc ccgcggggac cgcagggcca gccaggccaa gacctcaccc
     1561 atcttgtggc tgtaggggcg ggcctccaga atccgctcct gcgcgcggcg gagcttggcc
     1621 gccgccacca gcttcatggc gcgggtgatc tgctgggtgc tcttgacgct ggtgatgcgt
     1681 cgtcggatgt cgcgcagggt cgccatggat ctagaatgca ggcttcagga aagagttatc
     1741 gaatcgccaa ccaccaatcc aaaatccgga cgacgtcagg ccgccttggc cgtcgccagg
     1801 aactccgcct tgcacgcctg gatgcactca tccatccggc cgcgcagggc atcaccgatc
     1861 tccttcttct ccaccaactc ccggtagatc tcggggtggc gcatctcggc gaacttgtag
     1921 agcgtctcct cgaagctggc cacgctctcc gcgggcagat cgtccaggtg ccccgaggtc
     1981 ccagcgtgga tgatgaggac ctgccgctcg accggcaccg gccggtactg accctgcttc
     2041 agcacctcca ccatgcgctg accccgattg agctgggcct gggtggcctt gtccagctcc
     2101 gatccgaact gggcgaaggc cgccatctcc cggtactggg ccaggtccag tcgcagcttg
     2161 cccgccacct gtttcatggc cttgatctgc gcggccccgc ccacgcggga caccgacagg
     2221 cccacgttca cggccgggcg gatgccggca tagaacaggt ccgtctccag gtagatctgg
     2281 ccgtcggtga tggagatgac attggtggga atgtacgccg acacgtcgcc gagttgggtc
     2341 tcgatgatgg gcagggaggt gagcgagccg cctcccagtt gatcgttgag cttcgccgcg
     2401 cgttccagga ggcgggagtg gaggtagaac acgtccccgg gataggcctc gcgccccggc
     2461 ggccgccgca agaggagtga gagctgccgg taggccgccg cgtgcttcgt caggtcgtca
     2521 tagatgcaca gggcgtggcg cttggtatcg cggaagtact cgcccatggt gcagccggtg
     2581 tacggggaga tgtactggag cggcgctggc tcggacgccg aggccaccac cacggtggtg
     2641 taggccatgg cccccgcctc ctccagcacc ttgaccacct gggcgacggt ggagcgcttt
     2701 tggcccgtgg ccacatagaa gcagtaaacg tccttgccct tctggttgat gatggtgtcc
     2761 actgcgatgg cggtctttcc cgtctggcgg tcgccgatga tcagctcgcg ctggccccgg
     2821 ccgatgggga tcatggcgtc gatggccttg atgccggtct ggagcggctc cttcacgggg
     2881 cgccggtcca ccacgccagg cgccaggcgc tcgatggggt tgaactgcgt ggtaatgatg
     2941 ggacccttat cgtcgatggg ttggcccaag gcgttgacga cccgcccaat catggcctcg
     3001 cccactggta cggaca
//
LOCUS       DBNGZC010001099         4618 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2537599, whole genome shotgun sequence.
ACCESSION   DBNGZC010001099 DBNGZC010000000
VERSION     DBNGZC010001099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2537599"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="AC16G0_18975"
     CDS             <1..394
                     /locus_tag="AC16G0_18975"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HIR5101745.1"
                     /translation="VGVATAMIDVSDGVASDANHLCSESRVGIRLQAGRMPIHPGARV
                     MARLLGQEALDLALRGGEDYELLFAASADPVARLAEAAPGLPVTWVGEVTPGPPVPSL
                     VHADGREEVLAGGFDHFKGSHLHAGRSG"
     gene            476..898
                     /locus_tag="AC16G0_18980"
     CDS             476..898
                     /locus_tag="AC16G0_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101746.1"
                     /translation="MGLNLWTRRLRLPAALRGAVTIVAFTESGQMRRMRLPLRLVAIA
                     VLLLLSLITGSMVSLISVFRGQVDFARMTYLERENQSLTSLLEGQAEQLSRLKVEVAR
                     LREFEKNLRVVSGMDSQTEPIVGTGQARGAKPESPRKR"
     gene            966..1865
                     /locus_tag="AC16G0_18985"
     CDS             966..1865
                     /locus_tag="AC16G0_18985"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HIR5101747.1"
                     /translation="MSPGAEGSGSSIHAKLEAEAFEGLRTLVLTAGGIDLNLYKDRCV
                     LRRIAVRQRACGAPDLRAYLRLIAHDPVERGRLVKTLTIHVSQFFRNLTAFRAIQDEV
                     LPAILRQKRAGGGRALRFWSVGCACGEEAYSLAILLLAEASDAVREYPSTIFGTDIDP
                     DCLQHAKRGRYPEGSLAHVPAAWKRRFFVQTGSAYEVVPEVRRLVYFKVHNVLDPPPH
                     SRIDLLVFRNVLIYMTEPLQESVLLSLYEALNPGGFLVLGKVEGLTGTVRDCFEMVNV
                     AERIYRKPVQSSPRPGPQARDLE"
     gene            2007..3275
                     /locus_tag="AC16G0_18990"
     CDS             2007..3275
                     /locus_tag="AC16G0_18990"
                     /inference="COORDINATES: protein motif:HMM:NF012245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR5101748.1"
                     /translation="MESSTTIGKKLYAGFLLVIFATAVLSTIFSLVLRKFIPQEYIMI
                     ITWLIAIVAGMVLAFLMSRSLTTEIRSLATTARTVAEGNLTRDVSVNTSDEVGELATA
                     FNQMVGSLREVVREVKTTSQAVTTSAVALSASAEEMNSSTEEIAGTVEQIAKGAEHQA
                     SLVERTSKVIREMADAVTEIATRAKSAAEAAAEAGYTAQTGGKSAQEAMDKMKGVFSI
                     IEGAAGGVKNFSEKTQQIGSIVDVITKIAQQTNLLALNATIEAARAGEAGRGFAVVAE
                     EVRKLASEAGGSAEKIAELIKQVQTENTKVAASMEVATREMTSGREVLTYTSDALQDI
                     VRVVVEEVKKVQEISALTQQQTQGAQEMVKTIDEIAKVAEDNAASTEEASAATTEQTA
                     SMEQMARSAQQLSGLADKLKTMVARFNVGA"
     gene            3262..3801
                     /locus_tag="AC16G0_18995"
     CDS             3262..3801
                     /locus_tag="AC16G0_18995"
                     /inference="COORDINATES: protein motif:HMM:NF013731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HIR5101749.1"
                     /translation="MSGRDSEEHSLVPLDWDATTETTEDLQRFVLFQACDEWFGLPIQ
                     SLREIQPLERVTRVPNAPKELLGILNLRGRILALFELGQCLGIPPGTQPNTHAVVLDV
                     GDPELQVGFAVQRIAQIQGLAPSAIGPPPPREGGPSGVEGVCEVEGQVVSLLAPARVF
                     ARPLSDWGVTVEIQGPVGG"
     gene            3826..4242
                     /locus_tag="AC16G0_19000"
     CDS             3826..4242
                     /locus_tag="AC16G0_19000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006692844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5101750.1"
                     /translation="MSIKILVVDDAIFMRSMIRDIFARGPFVIAGEAENGTEAVRLYR
                     ELGPDLTTMDIVMPQMDGITALKEIIQMDPAAKVVMCSALGQEALIAESIEAGACDFI
                     IKPFQPARVLKVVQSVLGLDEQFQPVTTLRPRGEAR"
     gene            4239..>4618
                     /locus_tag="AC16G0_19005"
     CDS             4239..>4618
                     /locus_tag="AC16G0_19005"
                     /inference="COORDINATES: protein motif:HMM:NF013502.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HIR5101751.1"
                     /translation="MSRNDGERLGNEQRQLPAPVGLAVGASAGGPRALLTLLAMLDLP
                     APIFAFLVQHIHPKFTRILVRRLSEVSAFPVQLAEDLASVEPGRLYLAPGGRHLVVER
                     VRPVTYRTRLTDDPPRHGVRPAVD"
BASE COUNT          815 a         1444 c         1576 g          783 t
ORIGIN      
        1 ggtgggtgtg gccacggcca tgatcgacgt gagcgacggt gtcgcctccg acgccaacca
       61 cctttgcagc gagagccgtg tcggcatccg tctgcaggca ggccgcatgc ccatccatcc
      121 cggcgccagg gtgatggccc ggctccttgg ccaagaggcc ctggaccttg cgctccgcgg
      181 cggggaggac tatgagctcc tgttcgcggc cagcgccgat ccggtggcgc gtttggcaga
      241 ggccgcaccg ggacttcccg tgacctgggt cggcgaggtg acccccggtc cgccggtccc
      301 cagcctggtc cacgccgacg gcagggagga ggtcctcgcc ggaggcttcg atcacttcaa
      361 ggggtctcat cttcacgctg gacgcagcgg ctgaccgccg ccgattccca agcggccaga
      421 gccgcgaggg cgtgcgatca ggatatcggc ctggatttga tgaaaggggg catctatggg
      481 tctgaacctc tggacccgcc ggctccgtct cccggctgcg cttcggggcg cggtgaccat
      541 cgtggcgttt accgagtcag gccagatgcg tcgcatgcgg ctgcctctgc ggcttgtggc
      601 tatcgccgtc ctccttctcc tgagcctgat caccggatcc atggtgtcgc tcatcagcgt
      661 cttccgcggg caggtggatt tcgcccgcat gacgtacctg gagcgggaga accagtcgct
      721 cacctccctt ctggaggggc aagcagagca actgagtcgc ctgaaggtgg aggtcgcacg
      781 cctccgggag tttgaaaaga acctccgcgt ggtctcgggg atggactccc aaacggagcc
      841 aatcgtggga accggccagg cgcggggcgc caagccggaa tcgccccgga aacgataggg
      901 gaccggacgc gtcgctggga ttgacacggc ccgagtggag tcctgagggg gtgcggggcg
      961 gagggatgag ccctggcgca gagggatcag gatcgagcat ccacgccaag ctggaggcag
     1021 aggccttcga agggctccgg accctcgttc tcacggccgg cggcatcgat ctcaacctgt
     1081 acaaggaccg gtgcgtcctt cgccggattg ccgtcaggca gcgggcctgc ggggcgcccg
     1141 atctccgggc ctatctcagg ctgattgctc acgacccggt ggagcggggg agactcgtca
     1201 agacgctgac gatccatgtc tcccagttct ttcgcaatct caccgcgttt cgtgccatcc
     1261 aagacgaggt cctgcccgcc atcctcaggc agaagcgggc gggcggcggc cgcgcgctgc
     1321 gcttctggag cgtgggctgc gcctgcggag aggaggcata ctctctggcc atcctgctcc
     1381 tggcagaggc gtcggacgcg gtgcgggagt atccgagcac catcttcggc accgatattg
     1441 acccggactg cctgcagcat gcaaagaggg gacggtatcc cgagggcagt ctggcccacg
     1501 tgccggctgc ctggaagcgg cgattctttg tgcagaccgg cagcgcctac gaggtcgtcc
     1561 ccgaggttcg cagactcgtc tacttcaaag tccataacgt tctggaccca cccccgcata
     1621 gtcgcatcga cctcttggtc ttccgcaacg ttctgatcta catgacggag cctctccaag
     1681 aaagcgtgct cctgagcctt tacgaggcgc tcaatcccgg cgggttcctg gtgctgggca
     1741 aggtggaggg cctgaccgga acggtccgcg actgtttcga gatggtcaac gtcgccgagc
     1801 gcatttaccg gaagccggtc caatcctcgc cccgaccggg accgcaagcc agagatctcg
     1861 aatagcgcaa aggtcccgcc aatacgggct ttccccttga cttggcgatt tggggtaggt
     1921 atggtacgaa ccgttcacat tggcgtgctg cgtcatcatg cgtatgaggt agtgcggcgc
     1981 gttgcgcatg tgcggggagg catagaatgg aatcttcgac gacgatcggc aagaaactgt
     2041 acgctggctt tctcctggtc atcttcgcga ccgcggttct ctccaccata ttctccctgg
     2101 tcctgcggaa attcattccg caggagtaca tcatgatcat cacctggttg atcgccatcg
     2161 tcgccggcat ggtgctggcc ttcctgatgt ccaggagtct gacgacggaa attcgatccc
     2221 tggccacgac tgctcggacg gtggcagagg gcaacctcac ccgggacgtg tccgtgaaca
     2281 cctcggacga ggtgggcgag ctggctacgg cgttcaacca gatggtgggg agtctgcggg
     2341 aggtggtgcg cgaggtcaag accacgtccc aggctgtcac cacctctgcc gtcgccttgt
     2401 ccgcctcggc ggaggagatg aactcctcta ccgaggagat cgccggcacg gtcgagcaaa
     2461 ttgccaaggg tgccgagcac caggcaagcc tggtggagcg gacctccaag gtcattcgag
     2521 agatggccga cgccgtcacc gagatcgcga cgcgggccaa gtcggccgcc gaggccgcgg
     2581 ccgaggcggg gtacacggcg cagaccggag ggaaatccgc ccaggaggcc atggacaaga
     2641 tgaagggcgt cttcagcatc atcgaagggg ccgccggtgg ggtcaagaac ttcagcgaga
     2701 agacacagca gatcgggagc atcgtggatg tcatcaccaa gatcgcccag cagaccaacc
     2761 tgctcgcgct gaatgccacg atcgaggcgg cccgggcggg agaggctggt cggggcttcg
     2821 ccgtggtggc ggaagaggtc cggaagctgg ccagcgaggc gggcggctcc gccgagaaga
     2881 tcgccgagct gatcaagcag gtccagacgg aaaataccaa ggtggcggcc tccatggagg
     2941 tggccacgcg ggagatgacc tcgggccgcg aggtgttgac ctacaccagc gacgccctgc
     3001 aggacattgt gcgggtggtg gtggaggagg tcaagaaggt ccaggaaatc tcagccctca
     3061 cccagcaaca gacgcagggt gcccaggaga tggtgaagac gatcgacgag atcgccaagg
     3121 tggcggagga caacgcggcc tcgacggaag aggcctcggc tgcgaccacc gagcagaccg
     3181 cctccatgga gcagatggcg cgctcggccc agcagttgag cggtttggct gacaaactca
     3241 agacgatggt ggcgcgcttc aatgtcgggg cgtgattccg aggaacacag cctagtccct
     3301 ctcgattggg acgcgactac cgagacgacg gaggacctcc agcggttcgt cctgttccag
     3361 gcctgcgatg aatggttcgg tctgcctatc cagtcgctcc gggagatcca gcccctggag
     3421 cgagtgacgc gggtgccgaa cgctcccaaa gagctcctgg gcatcttgaa cttgcgaggg
     3481 cggatcctcg ccctcttcga actgggccag tgcctgggga tccctcccgg aactcagccg
     3541 aacacccacg cggttgttct ggatgtcggc gacccggaac tccaggtcgg cttcgcggtt
     3601 cagcggatcg cccaaattca gggactcgcc ccctcggcca tcggcccccc ccctccgcgt
     3661 gagggggggc cgagcggtgt ggagggggtt tgcgaggtgg agggtcaggt ggtgagtctc
     3721 ctggctccag cgcgggtctt tgcgcgaccg ctgtcggact ggggggtgac ggtggagatc
     3781 caggggccgg tggggggctg accccgctga cgagtggagg cagccgtgag cataaaaatc
     3841 ctggtcgtgg acgacgcgat cttcatgcga agcatgatcc gcgacatctt tgctcgcggg
     3901 cccttcgtca tcgccggcga ggcggagaac gggacggagg cggtgcgtct gtaccgggaa
     3961 ttgggcccgg atctgacgac catggacatc gtcatgcccc agatggacgg tatcacggct
     4021 ttgaaggaga tcatacagat ggacccggcc gccaaggtgg tgatgtgcag cgccctaggg
     4081 caggaggcct tgatcgcaga atccatcgag gccggcgcct gcgacttcat catcaagccc
     4141 tttcagccag cccgcgtgct gaaggtggtg cagagcgtcc tgggcctcga cgagcagttc
     4201 cagcccgtga cgaccctccg ccctcgggga gaggcgcggt gagccgcaac gacggcgagc
     4261 gactgggcaa cgagcaacgc caactgccgg cgcccgtggg gctggccgtc ggggcgtccg
     4321 cgggaggccc cagggccctc ctcaccctct tggccatgct ggacttgcca gcccccatct
     4381 tcgccttcct ggtccagcac atccatccca aattcacccg catcctggta cgccggctgt
     4441 ccgaggtctc agcattcccc gtccagctgg cggaggatct cgccagcgtg gaacccggac
     4501 gattgtactt ggcgcccggg gggcgccacc tggtggtgga gcgcgtccga ccagtcacct
     4561 atcgcacgcg cctgaccgat gacccgccgc gccacggggt gcgtcccgcc gtggacga
//
LOCUS       DBNGZC010001100         2988 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1467019, whole genome shotgun sequence.
ACCESSION   DBNGZC010001100 DBNGZC010000000
VERSION     DBNGZC010001100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1467019"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="AC16G0_19010"
     CDS             <1..473
                     /locus_tag="AC16G0_19010"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5101752.1"
                     /translation="AVHVDAADTRRMLESGVSVAHCPRSNTRLSEGVAPVAEFLRQGI
                     PVGLGTDSLASVPSLDLWDEMRAALAAHAGRLSPEGVLGLATLGGARVLGLENSIGSL
                     APGKQADLIAVAAEAVKASDPIGSLIAGTRGESVLLSLIEGEVRFSRSEGMPCA"
     gene            464..1303
                     /locus_tag="AC16G0_19015"
     CDS             464..1303
                     /locus_tag="AC16G0_19015"
                     /EC_number="4.2.1.151"
                     /inference="COORDINATES: protein motif:HMM:NF014658.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthetic enzyme MqnA/MqnD family
                     protein"
                     /protein_id="HIR5101753.1"
                     /translation="MRLRLGRVRYINCEPVYYGLEQGLVAADCDILDGTPAELNGLLR
                     AGDLDVSVVSAMEAALRPAAYRLLPDLAIACDGPVESVQLLCERPVEELDGQAVSLTR
                     HSLTSVYLVKLLLEKAYQVRPRFVADDQAAEARLVIGDEALRLGPTCPHPLDLGKAWH
                     ALTGLPFVFAVWAVRAEVWAARTDEVRRLHQALLQSKAYSRAHPEAVLTLAAARVDMP
                     PEACRRYLRERLCFDLGPRHLVGLRTFLQMLADAGALPAMPSLSFISSGDPTEDTAWR
                     AGA"
     gene            1285..2097
                     /locus_tag="AC16G0_19020"
     CDS             1285..2097
                     /locus_tag="AC16G0_19020"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HIR5101754.1"
                     /translation="MAGGRLSFIQISDLHILADDDRSQYGAKTAAILQQAVPLLNALR
                     PDFVVASGDLVSDESGESYRRLQALMSPLEAPIHLMLGNHDDRVVFRQIFRPTETPSR
                     QPVYEAFEQAGIRFLLLDSGLPGKEEGLLDPGQLRWLETELANHPDQPTWLFLHHQPL
                     PIYIRWLDQLGLQNREQLLAILARHRQLKVVGYGHIHQSRRWRYGHTLFAGVPALAFQ
                     FSAVSQEPQITLDTPAFRRFEVADGEHRSWLHFLDGRVVAEPGLLSTPIYVR"
     gene            2226..2975
                     /locus_tag="AC16G0_19025"
     CDS             2226..2975
                     /locus_tag="AC16G0_19025"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR5101755.1"
                     /translation="MAKEFARCGLAVSEEPFEVEGSRFRNIVGTCAGTTAAEERILVV
                     AHYDTKPGTPGADDNASAVAAMLEVARLLAPRRWQRTLQFVGFTLEEYGYQGSRRFAR
                     QAKREGRQILGVLDLEMVGFTSQRQRLPAGIRARTSGDFIGVVGNRRSRGLVEAFAAS
                     ARQSVPTLPVETLIVTWNGRLLPIVRLSDHAPFWDAGFAAVMITDTAFLRNPHYHEPS
                     DTLETLDLPFLTKVTTATAATAAHLVLPDLS"
BASE COUNT          454 a         1024 c         1006 g          504 t
ORIGIN      
        1 acgccgtcca cgtggatgcg gccgataccc gccgcatgct ggagagcggc gtgagcgtgg
       61 cgcattgccc gcggagcaac acccgcctct ccgagggcgt ggcgccggtc gcggagttcc
      121 tgcggcaggg gatccccgtg ggattgggaa cggactccct ggccagtgtt cccagcctgg
      181 atctctggga tgagatgcgg gctgccctcg cggcgcacgc aggccggctc tcgccggagg
      241 gggtgctcgg cctggcgacc ctgggcgggg cccgggttct cgggctcgaa aattccatcg
      301 ggagtctcgc gccgggtaag caggccgacc tgattgccgt ggcggcggag gcggtgaagg
      361 catcggaccc catcggatcg ttgatcgccg ggacgcgggg agagagcgtg ttgctcagct
      421 tgatcgaggg ggaggtccgg ttcagccgga gcgagggaat gccgtgcgcc tgagactggg
      481 tcgggttcgc tacatcaact gtgagccggt gtactacggg ctggagcagg ggttggtcgc
      541 cgccgattgc gacatcctgg atggaacccc ggcagagttg aacggcctgc tccgcgcggg
      601 ggacttggat gtctcggtgg tctcggccat ggaggccgcc ctgcgccctg cggcctaccg
      661 gctgctgccg gatctggcca tcgcctgcga cggtccggtg gagagcgtcc agctcctctg
      721 cgagcggccg gtggaggagc tggatggcca ggccgtctcg ctcacccgcc actcgctcac
      781 gtcggtctat ctggtgaagc tcctattgga gaaggcgtac caggtccggc cgcgcttcgt
      841 ggcggacgac caggcggcag aagccagact cgtgatcggg gatgaggccc ttcgcctcgg
      901 cccgacctgc cctcaccccc tggacctggg gaaggcctgg catgccctga ctggacttcc
      961 cttcgtcttc gccgtgtggg cggtccgcgc cgaggtgtgg gccgcccgga cggatgaggt
     1021 gcgccggctg catcaggcgc tcctgcagtc gaaggcctac agccgggccc accccgaggc
     1081 cgtgctgacc ctggccgcag cgcgggtcga tatgccccct gaggcgtgtc gccggtatct
     1141 ccgggagcgc ctgtgctttg atctcgggcc gcgtcacctc gtgggcctcc ggacgtttct
     1201 gcagatgctg gccgacgccg gagccctgcc ggcgatgccg tccctctcat tcatttcgag
     1261 cggggacccg acggaggata cggcatggcg ggcgggcgcc tgagcttcat ccagatctcg
     1321 gacctccaca tcctggcgga cgacgatcgc tcccagtacg gggcgaagac ggccgccatt
     1381 ctccagcaag cggtccccct cctgaacgcc ctccggcccg atttcgtcgt cgccagcggc
     1441 gacctggtga gcgacgaatc gggggagtcc tatcgacgtt tgcaggcgct catgtcgccc
     1501 ctggaagcgc cgattcacct catgctcgga aaccatgacg atcgggtcgt cttccggcag
     1561 atcttccgcc cgaccgagac cccgtcgcgc cagcccgtgt atgaggcgtt cgaacaggcc
     1621 ggtatccgct ttctcctcct ggactcgggg ctgcccggca aggaagaagg gctcctcgac
     1681 cccggccaac tccgctggct ggagacggag ctggcgaacc atcccgacca gccgacctgg
     1741 ctgttcctcc accaccagcc gctacccatc tacatccggt ggctggacca actgggcctg
     1801 cagaaccggg agcaactcct cgccatcctg gcccgccacc ggcaactcaa ggtcgtgggc
     1861 tacggccaca ttcaccagtc acggcgctgg cggtacggcc acacactctt cgcgggcgtc
     1921 cccgcgctgg ccttccagtt ctccgcggtg agccaagagc cacagatcac gctggatacc
     1981 cccgccttcc gccgattcga ggtcgccgac ggcgaacatc gatcgtggct gcactttctg
     2041 gacggccggg tcgtggccga gcccggactt ttgtcgacac ctatctacgt tcgttgatgc
     2101 cgtcgcccga catgacccag gatacgactg cctcgcagaa gagacttcat gcagacctcc
     2161 tggccctcat cgggccgcga gaccctcatg cgaacctcca cgcgttaaac gcggccgcgg
     2221 atcatgtggc gaaggaattc gcgcgctgcg ggctcgcggt ctcggaggag cccttcgagg
     2281 tggagggcag ccggttccgg aatatcgtgg ggacctgcgc cgggacgacg gcagcggagg
     2341 agcgaatcct ggtcgtggcc cactacgata ccaagcccgg gacgcctggc gccgacgaca
     2401 acgccagcgc ggtggcggcg atgctggagg tggccaggct tctcgcgccc cgtcgctggc
     2461 agcggacgct tcaattcgtg ggcttcacgc tggaggagta tggctaccag gggagccgcc
     2521 ggtttgcgcg gcaggccaag cgggaggggc ggcagatcct cggcgtcctg gatttggaga
     2581 tggtgggatt cacgagccag cgccagcgcc tccccgcggg catccgcgct cggacgagcg
     2641 gggacttcat cggggtggtg ggaaaccggc gatcccgggg actggtcgag gccttcgcgg
     2701 catcggcccg gcagtccgtc cccactctgc cggtggagac gctcatcgtc acctggaacg
     2761 gaaggctact gcccatcgtc cgcctgagcg accacgcccc cttctgggac gcaggatttg
     2821 ccgccgtcat gatcaccgac accgccttcc tacgcaaccc ccactaccac gagccgagcg
     2881 ataccctgga gaccctggat ctccccttcc tcaccaaggt aaccaccgcc accgcagcca
     2941 cggcagccca tctggtccta cctgatttgt cctgattccg cttgacat
//
LOCUS       DBNGZC010001101         2079 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1561545, whole genome shotgun sequence.
ACCESSION   DBNGZC010001101 DBNGZC010000000
VERSION     DBNGZC010001101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1561545"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1172
                     /locus_tag="AC16G0_19030"
     CDS             129..1172
                     /locus_tag="AC16G0_19030"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5101756.1"
                     /translation="MTAKERDVRVGRREFLKWTGAAVAGLSLGRPSPAETAVAGKVLQ
                     YAGGSAESVTALDQLFTAKTGIPAESWRAGGVAVVQKVEAEFQAGRVLHNVIGNTEVE
                     VMIRWAEQGHLLNYDSPEASHFPREFRLPGYWVPQKVLLNTIAYNTGMVKPAEAPKHW
                     ADLMDPRWKDKIVMLDARASGSGVHLVYAMRKLLGKEFAARMAKQNVMLKRSGGDVSN
                     TVVAGERPIGAGIQEYYVYSRAKQGGPIAAILPDEGVPMTLYALCIPKNGPDLEAAKR
                     YVDFALGNEAQAMWQEKFATPVLRDDAPPYPREFGLRPLKEVKIIFSALEHLKEEGAT
                     KREFMDEWKQVSG"
     gene            1202..>2079
                     /locus_tag="AC16G0_19035"
     CDS             1202..>2079
                     /locus_tag="AC16G0_19035"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5101757.1"
                     /translation="MTVVRGPLWWAQAAVLLLLGYLILFPSVVLMLESVRDATGRWTM
                     AWYVQVYSTPGNYQALVNSLILGAGSAAFATILGTLLAWIVVRTDTPCRGLLEVAALV
                     PFICPPFIGALAWTLLGSPNAGLLNQWARKLLSIERLVNMYSLGGMVLVIGLYTAPFV
                     LLIVGGALRSMDASTEEASMMAGAGQAATTTLITLPMVAPAILAGAFLAFVLALEQFG
                     VPAVLGLPARIPVLTTKIWTTISEYPPQYGQGASLCVVLLLLTVAGVVLQRQLLRRRR
                     FTTVTGKGTRPRLVRLG"
BASE COUNT          326 a          670 c          731 g          352 t
ORIGIN      
        1 ggtgtcgccc tgcgggagcc tcattccccg cgacccggcg atcaggtgcg tggagggctg
       61 gcagcccacg gggagcacca gagcggaggc gtcataggtc accggacggt tcacaaggag
      121 ggcacgccat gacggccaag gaacgggatg tgagggtggg tcggcgggaa ttcctgaagt
      181 ggacgggggc ggcggttgcc gggctcagcc tgggtcgtcc ctccccggca gagactgcgg
      241 tcgccggcaa ggtgctgcag tacgccgggg gctcggcgga gtcggtgacc gctttggatc
      301 agctcttcac ggccaagacc gggatcccgg ccgaatcctg gcgtgccggc ggggtcgccg
      361 tggtgcaaaa ggtcgaggcg gagttccagg cgggccgggt cctccacaac gtgatcggca
      421 acaccgaggt ggaggtcatg atccgctggg cggagcaggg acatctcttg aactacgact
      481 cgcccgaggc ttcccatttc ccgcgggagt ttcgcctgcc gggctactgg gtgccccaga
      541 aggtcctgct caacaccatc gcgtacaaca cgggcatggt caagccggca gaggcgccca
      601 agcactgggc ggacctcatg gacccccgct ggaaggacaa gatcgtcatg ctggacgcgc
      661 gggccagcgg ctccggcgtc cacttggtct acgccatgcg caagctgctg ggaaaggagt
      721 tcgccgcgcg catggccaag cagaacgtca tgctgaaacg gagcggcggg gacgtgtcca
      781 acaccgtggt cgccggcgag cggcccatcg gcgcgggaat ccaggagtat tacgtgtact
      841 cccgggccaa gcagggcggt ccgatcgccg ccattctgcc ggacgagggc gttcccatga
      901 cgctctatgc cctgtgtatt cccaagaacg gacctgatct cgaggccgcc aagcgctacg
      961 tggactttgc cctggggaac gaggcgcagg ccatgtggca ggaaaagttc gccacaccgg
     1021 tgctgcggga cgacgccccg ccctacccgc gcgagttcgg cctccgcccc ctcaaggagg
     1081 tgaagatcat cttcagcgct ctcgagcatc tgaaagagga aggggccacg aagcgggagt
     1141 tcatggacga atggaaacag gtgtcaggct agggtcgtcg ccctgggcca ggcccacctg
     1201 ggtgacggtc gtccggggtc ccctctggtg ggcgcaggca gccgttctgc tcctcctcgg
     1261 gtacttgatc ctcttcccgt cggtggtcct gatgctggag tcggtccggg acgccacggg
     1321 acgatggacc atggcctggt atgtccaggt ctattccacc ccgggcaatt accaggcgct
     1381 ggtcaacagc ctgatcctgg gggcgggctc ggcggccttc gccacgatcc tggggacgct
     1441 tctggcctgg atcgtggtcc ggactgacac gccgtgtcgc ggcctcctgg aggttgccgc
     1501 gctcgtccca ttcatctgcc cgcccttcat cggggcgctg gcctggacct tgctggggag
     1561 cccgaacgcc ggcctgctga accagtgggc gcgaaagctc ctgagcatcg agcggctggt
     1621 gaacatgtac tcgctcggcg gcatggtcct cgtgatcgga ttgtacacgg ccccgttcgt
     1681 cttgctgatc gtggggggcg ccctccgctc catggatgcg agcacggaag aggcctccat
     1741 gatggccggc gcgggccagg cggcgaccac caccctgatc acgctcccca tggtggcgcc
     1801 cgcgatcctg gccggggcgt tcctggcttt cgtcctggcg ctcgagcagt tcggcgtgcc
     1861 ggcggttctc gggcttccgg cgcggattcc ggtcctgacg acgaagatct ggaccaccat
     1921 ctcggagtat cctccgcaat acggccaggg agcctccctc tgcgtcgtgc tgctcctgct
     1981 caccgtggcg ggcgtggtgc tgcagcgaca gctcttgcgg cggcggcgat tcaccacggt
     2041 caccggaaag ggaacgcgcc cccggctggt ccgactggg
//
LOCUS       DBNGZC010001102         6406 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1467351, whole genome shotgun sequence.
ACCESSION   DBNGZC010001102 DBNGZC010000000
VERSION     DBNGZC010001102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6406)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1467351"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(543..1508)
                     /locus_tag="AC16G0_19040"
     CDS             complement(543..1508)
                     /locus_tag="AC16G0_19040"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR5101758.1"
                     /translation="MSGAINAAMVIGAIFGAEIFLPSTRLGHALERASFLVTFVTKLL
                     VYGTLVVLVVGGRPGGRLAALGAAALFSPDLARAIYGETKVPTAQLMATWLLCVGVGI
                     FVLQMSRLIGYRTLVDILVGRYHRSRLEERFFLFIDIAGSTPLAERLGPAAVHRFLGE
                     VFRVASDPIDDHQGEVYQYVGDEIVITWTVANGRDAARPLACFFAVEQALERTASDFG
                     RSFGAVPRLRAALHAGSVITGEVGGSRRAIVYHGDVMNTASRMEQSARDLQRQFLVSG
                     DALERIAGLDGFTLEDLGLQQLRGRAAAMHVYAVTADSSRAVGRP"
     gene            complement(1836..2600)
                     /locus_tag="AC16G0_19045"
     CDS             complement(1836..2600)
                     /locus_tag="AC16G0_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin-like domain"
                     /protein_id="HIR5101759.1"
                     /translation="MISTEIALIVFTCVFGGALLGMFLRAVTPEHHLSQASQDVIKLG
                     MGLIATMAALVLGLVIATAKSSYDSQDAAVKHSAAKVLLLDRVLANYGPETREARDLL
                     RRTVANRLEAIWPEDRSQPAGLGGAPEVESIAQGIEARILQLAPQNETQRWLRSQALQ
                     IGSDIMETRWLMLGGLGGSVPLPFLVVVVFWLTIIFGSFGLFAPRNGTVVTVLFLCAL
                     SVAASIFLILEMDRPFEGLMKISSAPVRYTLSQLGQ"
     gene            3390..4559
                     /gene="lptF"
                     /locus_tag="AC16G0_19050"
     CDS             3390..4559
                     /gene="lptF"
                     /locus_tag="AC16G0_19050"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HIR5101760.1"
                     /translation="MKLIDRAILREMGLAFSLGLMVYTFVLLTNKILRLVELIVNKGV
                     GIFTVLQLFLYILPYSLVVTIPMSVLLATLTTYSRLGADGEILALRGAGLSLIRLTRP
                     AVWFGLASMLLTFTITIWILPYSNHAFKNLVFQMARRQATVGIQEGIFNADLEGLILY
                     VEHVDPRTSKLEGVFLVDNRNPAERRVIVAQSGQFTSDPKNLRMGLDLSQGSIQLSTK
                     ELVGRYRLLSFADYHLTFDLGRGLGDPIQRSPGEQELTLAELRERAASLRAQGQNFHP
                     PLVELHKKLAIPFSCLLFTLVGVPLGSRIRRVGRGLSLAISVGCALGYYMLIVAGEGL
                     GDRGLVPEALAMWLPNLLIAMGGSLLLMWAEIHPATLRQALTSRQFRPAVNGPRG"
     gene            4565..5641
                     /gene="lptG"
                     /locus_tag="AC16G0_19055"
     CDS             4565..5641
                     /gene="lptG"
                     /locus_tag="AC16G0_19055"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HIR5101761.1"
                     /translation="MRLLDRYIARECMKILLLCLVVFMGVYLIVDLFEKFSRFLEAQM
                     PPALILRYYLFRLPKILTEVLPVAVLLASLLSLGGLARHNELLAMKMGQVGTLRIALP
                     CMVLGLLASGIAWVTTEYVSPRTNERALNIERTQVRRLPAHQTTRDSDIWYRAQGNRF
                     VHISLIETESGLIRGMSVFQLSPASELVQRMDAQEATWGPDGWTLRDGWTLDLTRNPI
                     RVAPFQTMRIALPERPEEFARVARSPEEMSYVQLRRYIDQLVQSGVSATRYRVDLYAK
                     VAIALVSLVMALIGVSFGLRTGKGGAMLWVGVCIPMGFLYWMLLSVGVSLGRGGVLPP
                     FVAAWLPNFVFGLGGLFSLWRLRG"
     gene            complement(5834..>6406)
                     /locus_tag="AC16G0_19060"
     CDS             complement(5834..>6406)
                     /locus_tag="AC16G0_19060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR5101762.1"
                     /translation="MGADVIVTEIDPLKSIEAVMDGYRVMSMAEAARIGDVFVTVTGN
                     KNVLHKEHFEAMKNGAIVANSGHFNVEIDIPALEKMASSRRPMREFVEEFALKDGKRI
                     YLLGDGRLINLAAAEGHPASVMDMSFANQALASEYAVKHRGKLERKVHRVPMAIDKEI
                     ARLKLESMGVSIDTLTEEQRKYLESWEAGT"
BASE COUNT         1087 a         2156 c         2013 g         1150 t
ORIGIN      
        1 agctgggtgt ggcgcgtcgc gccgagggcg cagccttcct gggcaggtcg atgcttccgc
       61 agccacggcg tcccgaagcc ggctggcccg cgggcccggc gggctcgggg cgagccggaa
      121 cggcctcaat catgtggtca acttcaggga caggacacta ggtcgaatgg aaccgctgaa
      181 ctggaatacg tccgtgaacc acgcacctgc actgaagctg cgtgaccagt agaccgcgat
      241 gatcagtctg ccgctcacag gcaggtccac agggagcgta aacatccgta tccggtcgag
      301 aaaaacgaaa ggaagacagg gcgccccacg agaaccagga tcgcctccgc gacttcctgc
      361 acgttcgttc ccgactgcct cttcgtgacg cccatcaggc gctccccaga atgcaggcca
      421 cctccgggac tggcgcccag ccccgtgggg cccaacgaaa tacccgtgag cggtcggtgt
      481 cgatcactcg ctcgtcgcga cagcctacat gccggtccgc tcgattggcg tgtcagccct
      541 cgtcaaggtc ttccgaccgc tcgcgaagag tccgccgtga ccgcgtagac gtgcatcgcc
      601 gcggcacgac cacgcaattg ctgcaggccg aggtcctcca gcgtgaagcc gtcgagaccc
      661 gcgatccgct ccagcgcgtc acccgagacc aggaactggc gctggaggtc tcgcgccgac
      721 tgttccatgc gtgatgcggt gttcatcacg tcgccgtggt agacgatcgc gcgccggctg
      781 ccccccactt cgccggtgat gaccgatcca gcgtgcagcg cagcgcggag acgcggcacc
      841 gcgccgaagg agcggccgaa gtccgacgca gtccgctcca gcgcctgctc gaccgcgaag
      901 aagcacgcga gtggtcgagc ggcgtcgcgt ccatttgcca cggtccaggt gatcacgatc
      961 tcgtcgccga cgtactgata gacctcaccc tggtgatcgt cgatcgggtc cgaggccacg
     1021 cggaagactt ctcccagaaa gcgatggacc gcggcggggc cgaggcgctc ggcgagtggc
     1081 gtcgaaccgg cgatgtcgat gaagaggaag aaccgctcct ccaggcgcga gcggtgatag
     1141 cggccgacca ggatgtcgac cagggtgcgg tacccgatca gacggctcat ctggagcacg
     1201 aagatgccga cgcccacgca caacagccaa gttgccatga gctgggccgt cgggaccttg
     1261 gtctctccgt agatcgccct tgcgaggtcg gggctgaaca gcgcggcggc gccgagagcg
     1321 gcgaggcgcc cgccaggtct gcctcccacg acaaggacga ccagggtgcc gtagaccagc
     1381 aacttggtca cgaaggtgac gaggaacgac gcccgttcga gcgcgtgacc gagtcgtgtc
     1441 gatggcagga agatctcggc gccgaaaatc gccccgatca ccatcgcggc gttgatggcg
     1501 ccgctcaagg cgccctggag agaaccgagg agtggcgcct cgcggaacac ggagccaccc
     1561 gcacccccga agactgcgcc ggccaccaca ccccaagtca gcgcgcggac cagttgagcc
     1621 acccggcgtc gtgtgcctgt tcgcatcgtc ggcttctcag gcagccaggg aaatcgtcgg
     1681 ccggtgctcc atgcccatca tcgcctccac tgcccaacgc cagcgcacgc gacgcgcggc
     1741 acacagacta tggacgccgc caatacctcg ccccgcgcgt ccggttgacg cagagcttgg
     1801 gtggtggggc ctctgccgct cttcgcctcg agcgcctact ggccgagctg cgagagcgtg
     1861 tagcgcaccg gagcgctgga gatcttcatc agcccttcga acggccggtc catctccagg
     1921 atcaggaaga tggaggcagc caccgacagc gcacacagga acaggaccgt gaccaccgtt
     1981 ccgttgcggg gggcgaagag gccgaaactc ccgaagatga tcgtcagcca gaacaccacc
     2041 accacgagga agggtagcgg gaccgagccg cccaggccac ccagcatgag ccagcgcgtc
     2101 tccatgatgt cgctgccgat ctgcaaggcc tgggaccgga gccagcgctg ggtctcgttc
     2161 tgcggcgcga gttggagaat ccgggcctcg attccctggg cgatcgactc tacctccggc
     2221 gctcccccca accccgccgg ttgggagcga tcttccggcc agattgcctc gagcctgttg
     2281 gccacggtcc gtcggagcag atcccgtgcc tccctcgtct ctggcccgta gttggccagc
     2341 acccgatcca acagcaatac tttggcggca ctgtgcttga ccgccgcatc ctgcgagtcg
     2401 tacgaactct tggccgtggc gatcaccagc ccgagaacga gtgcagccat cgtcgcgatc
     2461 agccccatcc ccagcttgat gacatcctgg gaagcctggc tgagatgatg ttcgggcgtc
     2521 accgcgcgga ggaacatgcc gagcagcgcg ccgccgaaca cgcaggtgaa gacgatcaag
     2581 gcgatttcag tcgagatcac ctggagcctc ctcctgtctc aaggcgagtt gttgcggctt
     2641 ggatcgacga cgtcccctca tcctgccgcg ccctcggtct gcctttccgc tgacaagcta
     2701 cagccttgcg gttcttcgat cctgacctct cctggacaga gcgaccgcat agcacccaag
     2761 gctgtgacag tcgatcctcc cggaatatac gagtggtcac ggactcaagg gcgatttccc
     2821 cgggatcacg cacagaatcc gcataacatc cctcgcatgc gaccctggtt acacggcatc
     2881 caccgggctc cgaacctcat ggccgccgcg aggcaggaca tgggtgtaga gagtcgtatt
     2941 cttgacgtgc atgtcgaaag agagatccgc gagtatccga ggagaagttc cctccctcag
     3001 ccgggatagg ggcaggtgaa cgactttgcg gtaaaggctg cggacagttg gacagggcgc
     3061 ggatcccgaa ccgtcgggct ggtggaagga taaagtggtc ggcgtgccaa cggaagctgc
     3121 gcgcatcgag cgccccctgt atgtggcacg atcccgagcg ggattcctgg ctccttttac
     3181 cctgtgcgat tcgtcgtgtc aaccggggag ttgccctgga ggaccggcag cgaagcttgg
     3241 ccgtatccaa ggaagcagtc cccgaccagg gtgcccgagg ctggacgatc gtcttcggcg
     3301 ggtatgggtc cgagcaaaaa ctgctaggtc gcgtgtaacc gctgaagtac aatgcgcccg
     3361 tgccgttcgc ctggcaagtg gggagctgca tgaagctgat tgaccgcgcg atcctgagag
     3421 agatgggcct ggcgttcagc ctcggcctca tggtttacac cttcgtcctc ctgacaaaca
     3481 agatcctccg cctcgtcgag ctcatcgtga acaagggcgt gggcatcttc acggtcctcc
     3541 agctcttcct ctacatcctg ccctattcgc tggtcgtgac cattcccatg tcggtcttgc
     3601 tggccaccct cacgacgtac tcgcgtctcg gcgccgatgg agagatcctc gccctgcgcg
     3661 gcgctggcct gagcctgatc cggctcaccc gtcccgccgt ctggttcggc ctcgccagca
     3721 tgctgctcac cttcaccatc accatctgga tcttgcctta cagcaatcat gccttcaaga
     3781 acctggtctt ccagatggcg cggcggcagg ccacggtggg aatccaggag ggtatcttca
     3841 acgccgacct ggaagggctc atcctctacg tggagcacgt ggatccgcgc acctccaagc
     3901 tggagggggt cttcctggtg gataaccgca atcccgccga gcgccgtgtg atcgtggcgc
     3961 agagcggcca gttcacctcg gatccgaaga acctgcgcat gggcctcgat ctcagccagg
     4021 gctccatcca gttgtcgacc aaagaactgg tcggccgcta tcgcctcctc agcttcgccg
     4081 actatcacct gaccttcgat ctcgggagag gcctcggcga tcccatccag cgctcgcccg
     4141 gcgagcagga gctcaccctg gccgagctcc gtgagcgggc ggccagcctc cgtgcccagg
     4201 ggcaaaactt tcatcctccc ttggtggaac tccacaagaa gcttgccatc cccttttcct
     4261 gcctgctctt caccctggtg ggcgtccccc tggggagtcg gatccggaga gtggggcgcg
     4321 gcctgagtct ggcgatctcg gtgggatgcg ccctggggta ttacatgctg atcgtcgcgg
     4381 gcgagggtct gggcgatcgg ggcctggttc ccgaagctct agccatgtgg ctccccaacc
     4441 tcctgatcgc catgggtgga agcctcctgc tcatgtgggc ggagattcat cccgccaccc
     4501 ttcgccaggc cctgacctcg cggcagttcc ggcccgcggt caacgggcct cgcggctgat
     4561 cccggtgcgg ctcctggatc ggtacatcgc ccgtgagtgc atgaagatcc ttctcctgtg
     4621 cctggtggtc ttcatggggg tgtatctgat cgtggatctc ttcgagaagt tctcgcgctt
     4681 tctcgaagcc cagatgccgc cggcgctgat cctccgatac tatcttttcc gtttgcccaa
     4741 gatcctcacc gaggtcctgc ccgtggccgt gctcctggcc agcctgctct cgctgggcgg
     4801 cctggcccga cacaacgagc tcctggccat gaagatggga caggtcggca cgctgcggat
     4861 cgccttaccc tgcatggtct tgggcctcct ggccagcggc atcgcctggg tcaccaccga
     4921 atatgtgtcc ccccggacca acgagcgggc gctgaacatc gagcggacac aggtccgccg
     4981 tcttccggcg caccagacga cccgcgacag cgatatctgg tatcgcgccc agggaaaccg
     5041 cttcgttcac atctccttga tcgaaacgga gtccggcctc atccggggga tgagcgtgtt
     5101 tcagctctcg ccagcctccg aactggtgca gaggatggat gcgcaggagg cgacgtgggg
     5161 acccgacggc tggacgctcc gcgatggctg gacgctcgat ctcacccgga accccatccg
     5221 ggtcgcgccg ttccagacga tgcggatcgc cctccccgag cggcccgagg agttcgcccg
     5281 ggtcgcccgc tccccggagg agatgagcta cgtccagttg cggcgttaca tcgaccagct
     5341 ggtccagagc ggggtgagcg cgacccggta ccgcgtggac ctgtacgcca aggtcgccat
     5401 cgccctggtg agcctggtca tggccctcat cggcgtgagc ttcggcttgc ggaccgggaa
     5461 agggggggcc atgctctggg tgggagtctg catccccatg ggcttcctgt actggatgct
     5521 cctctccgtg ggcgtttctc tggggcgggg aggggtcctg cctccgtttg tcgccgcctg
     5581 gctgccgaac ttcgtgtttg gcctcggcgg cctgttctcc ctctggcgcc tccggggctg
     5641 acagccgcac aggcgcaggg gcgatagggc gcagagacct cccagcacgg tcggcacccc
     5701 ggaaacgaaa cagccgggaa gttggtggac ttcccggccg tggtatttgc cgttgtcttt
     5761 cgccccggtg cccctgcccc tcggcgcctc cggaccgtct cccctctcgg ctctcgcctc
     5821 tcagctctca gctctacgtc cccgcctccc atgactcgag gtacttgcgc tgctcctcgg
     5881 tcagggtgtc gatgctgacg cccatggatt cgagcttcag ccgggcgatc tccttgtcga
     5941 tcgccatcgg cacccggtgg accttcctct ccagcttgcc ccgatgcttc acggcatact
     6001 ccgaggccag cgcctggttg gcgaaggaca tgtccatgac gctggccggg tgtccctcgg
     6061 ccgccgccag gttgatgagc cggccatcgc cgagcaggta gatgcgcttg ccgtccttca
     6121 gcgcgaactc ctcgacgaac tcgcgcatcg ggcggcgcga gctggccatc ttctccagcg
     6181 ccgggatgtc gatctcgacg ttgaagtgtc cggagttggc gacgatggcg ccgttcttca
     6241 tcgcctcgaa gtgctccttg tggagcacgt tcttgttgcc ggtgacggtg acgaagacat
     6301 cgccgatgcg ggcggcttcg gccatggaca tgacgcggta gccgtccatc acggcttcga
     6361 tggacttgag cggatcgatc tcagtgacga tgacgtcagc gcccat
//
LOCUS       DBNGZC010001103         1090 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1271847, whole genome shotgun sequence.
ACCESSION   DBNGZC010001103 DBNGZC010000000
VERSION     DBNGZC010001103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1271847"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="AC16G0_19065"
     CDS             complement(<1..904)
                     /locus_tag="AC16G0_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5101763.1"
                     /translation="MAGPRLHYAWVVLAVTFVCLLTAAAVRSLPGILMHPLEAEFGWN
                     RASIGLAVSINVLLFGLAGPLLGRVMDRVGPRAVASAATLLIALGAAGTLGMTRPWHM
                     DLLWGLVVGIGSGGATMVMGSTVANRWFSRQRGLALGILGAATSAGQIVFTPTLMQLT
                     LRWGWRGSTLFIAGIVGILVFPLVALFLRNGPADIGLQPYGASALAAGGAPPPPDPHP
                     MRAAVRTGDFWLLAGSFAICGLTTSGLIQTHLISHGTEHGFSEMTMAATLGVMGATDV
                     VGTIASGWICDRFGARGPLAFYYLF"
BASE COUNT          210 a          391 c          350 g          139 t
ORIGIN      
        1 ggaacaggta gtagaaagcc agaggccctc gggcgccgaa ccggtcgcag atccagccgg
       61 atgctatcgt ccccacgacg tccgtcgccc ccatgacgcc gagcgtggcc gccatggtca
      121 tctcggaaaa cccgtgctcc gtcccgtggg agatcaggtg cgtctggatc aggcccgagg
      181 tcgtgagtcc gcagatggcg aagctccccg ccaggagcca gaagtctccc gtccggactg
      241 cggcgcgcat gggatgggga tcgggtggag gcggagcccc cccggcggcc aacgcgcttg
      301 ccccgtaggg ctggagccct atgtcagccg gcccgttgcg gaggaacagc gccaccaagg
      361 gaaagaccag gatgcccacg atgcccgcaa tgaagagcgt gctcccccgc cacccccagc
      421 ggagggtgag ctgcatgagc gtcggggtga agacgatctg cccggccgag gtcgccgccc
      481 ccaagatccc cagggcgagg ccgcgctgac ggctgaacca ccggttggcg acggtggacc
      541 ccatgaccat ggtagccccg ccggacccga ttccgaccac gaggccccag agcaggtcca
      601 tgtgccaggg ccgggtcatg ccaagcgtgc ccgccgcccc gagcgcgatc aggagggtgg
      661 ccgcgctggc cactgcccgg gggcccaccc ggtccatcac ccgccccagc agggggccgg
      721 caaggccgaa caggagaacg ttgatcgaaa cggccagccc aatcgacgcc cggttccagc
      781 cgaactccgc ctccagcggg tgcatcagga ttcccggcaa agaccgcacg gcggcggccg
      841 tcaagagaca gacgaaggtc acggccagta caacccaggc gtagtgcaga cgcgggccgg
      901 ccatgggccg cacaggccct actcccactc cccttgatgc cataccacac actcctttgg
      961 tgggcgtccc acgaaggctc agccaccatg ctcggagaca tctccctgac gctcgctact
     1021 atgctgtgtc gatggccagt aagtgtatct acacccattg acccaataaa cgacctagcc
     1081 gggcctcacc
//
LOCUS       DBNGZC010001104         6423 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2247702, whole genome shotgun sequence.
ACCESSION   DBNGZC010001104 DBNGZC010000000
VERSION     DBNGZC010001104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 6423)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6423)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6423
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2247702"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="AC16G0_19070"
     CDS             <1..497
                     /locus_tag="AC16G0_19070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013932306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIR5101764.1"
                     /translation="GDTAQLTKVPAFQAEAKKWLVTAWEDVEDWSAAILTFEDGTKAT
                     VMSTDVSLGGVKNLVTAYLSNSVVQVNINPNTSLQIYAPDGQIWGDEYLTEKVETKSG
                     WQFPSPDEDWMRGYPQEMEDFVDAVREGREPLSGLLLAHETVEVIYAAYVSAQEGRRV
                     ELKR"
     gene            861..2351
                     /locus_tag="AC16G0_19075"
     CDS             861..2351
                     /locus_tag="AC16G0_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HIR5101765.1"
                     /translation="MNEILLGKGEQPVHLLAKYSNRHGLIAGATGTGKTVSLSVLAEG
                     FSRLGVPVFMADVKGDVAGLALAGASSEKIKARVAEIGIEGFAHEASPVVFWDLYGQA
                     GHPVRATVSEMGPNLLGRILQLNDTQAGVLEIAFQLADDRGLLLLDLDDLRALLGFLA
                     DNHQEISTKYGLVSTQSVAAIQRALLSLEREGGDALFGEPALELGDLLRTDLSGRGVV
                     NILAADRLILKPQLYSSFLLWLLSELFENLPEVGDLDKPKLVFFFDEAHLLFNEAPPA
                     LRQQVEQVVRIIRSKGVGVYFCSQFPDDVPNEILGQLGNRIQHALRAYTPRDQKAVRT
                     AAETFVANPKLDVAKVISQLGVGEALVSTLLEKGVPMPVERTMMCPPRCRIGAITPDE
                     RAAVRARSPVGAKYDSRVNRESAYEILTRRTVSPEAPPTSPDRARAEKPSGAGGQLGE
                     LLWGTKGRQGLVEAMAKQAARTVGSQIGRQILRGVLGGILGGSRGR"
     gene            2486..2887
                     /locus_tag="AC16G0_19080"
     CDS             2486..2887
                     /locus_tag="AC16G0_19080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101766.1"
                     /translation="MFFRAGAVWFMLLAIAVAAGAARTTLLQPQVGEQAAHVIGTLAG
                     VAIFAVVIWRTVPWIAPRSDRGQLVRVGIGWVAATVLFEFGFGRYVMGHPWSRLLADY
                     NLFAGRLWVLVLLTLLLMPIAAAEFRGRASR"
     gene            2989..3510
                     /locus_tag="AC16G0_19085"
     CDS             2989..3510
                     /locus_tag="AC16G0_19085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101767.1"
                     /translation="MGAISEALVDETWQEIGRLSETQARSRMDQIARAQPHLLAFVLG
                     TTGNLRADAQQVAVYLFAVIHRMFEKAVGRLTRAMPPEIDVVYERNQRFLEKFEHSHP
                     RFLEQAAKSMTSSQPFVMKYLVEALMETPEGDAPVALSEEETGLIFLVLKTVVDILDG
                     LAQRSSEAGDDTA"
     gene            3910..4725
                     /locus_tag="AC16G0_19090"
     CDS             3910..4725
                     /locus_tag="AC16G0_19090"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR5101768.1"
                     /translation="MKAREIHEYNQLTWREMNDAIEMQKVVILPTGSTEQHGPHLPLD
                     VDLFLAASVCHELGRRAPDKVLVLPAIPFGLNMHHIDFPGTIHIQPDHFINFCLDVTK
                     SVSYHGFKRIALVNGHGSNGPMVDLVARRTVLETPSICCAFGYFSMGWEEVLKLLESE
                     VHAHADEMETSLYLHLAKERVRLDQAVFGNDHVGHYMSSDSTKTVRFNDFWGRWTQTG
                     VHGDPTKATAEKGKQILEIVMKHMLAWLEEYRTWPIEPRSDQHRLPVQKEIRW"
     gene            4770..6323
                     /locus_tag="AC16G0_19095"
     CDS             4770..6323
                     /locus_tag="AC16G0_19095"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5101769.1"
                     /translation="MTRRELFAWIAGGILGFLLLFGFAGPAPAGTVLKIAADGDISDV
                     DLHMTTHYLNRVVLLNVYDMLFALGEDMGPKPSLVDKYTVSSDGLVYTLTLRRGVKFH
                     NGKTLDAKDVVYSITKMQNKGPRSGQFKKLIKSLEAADPLTVKIMLNEVNAGFIATLA
                     NPIAPAVIYPDGEAERQGGTITKPVGTGPFEFVEWKKDSVVRLKKFAGYTVDDRPTSG
                     FTGKRLELVDQVDFIPIRDSSVRAAALERGDVDVALALTQQDVERYKGKPGLTVAAKP
                     GVSFADLRFGFKKGIFMNNQKLRQAVGYAIDKEEIVKAVTFGLGKSAPAGIPVGTPYF
                     GAVHAADPFGKPNPEKAKQLLKEAGYKGEEIILDSHLQPSVYPEIAVVLQAQFQAVGL
                     NARVRTLESAALQTTGDGGEFAFFLSGLSPRPDAEIYYCQFWESSSTSTGYKNPEYDR
                     LCRAARTTMKQEERTKVFVDLETLLRTDVPWIPLIYLPQVEGWRSNVQGWQHWSAGYA
                     RAWNVTVGK"
BASE COUNT         1184 a         1973 c         2123 g         1143 t
ORIGIN      
        1 tgggcgatac ggcccagctc accaaggtcc cagccttcca ggccgaggcg aagaagtggc
       61 tggtgacggc gtgggaggat gtcgaggatt ggtcggccgc catcctcacc ttcgaggacg
      121 gcaccaaggc cacggtgatg tccacggacg tgagcttggg cggggtgaag aacctggtga
      181 ccgcctatct ctccaacagc gtggtccagg tgaacatcaa ccccaacacc agcctgcaga
      241 tctatgcccc ggacggacag atctgggggg acgagtacct cacggagaag gtggagacga
      301 aaagcggctg gcagtttccc agccccgacg aggactggat gcgcggctac ccccaggaga
      361 tggaggattt cgtggacgcc gtccgcgagg gccgcgagcc gttatcgggt ctccttctgg
      421 cccacgagac cgtggaggtc atatacgcgg cctacgtctc ggcgcaagag ggtaggcggg
      481 tggagttgaa gcggtaacgg accgcactga tagcctcggg tcgagcttca acaacacgtc
      541 gagaacgcgg gcctcgcgga ggattttgcc gaggaggcgg gcagcctcgg gcgaagagcc
      601 atccattccg tagcttgaca gcctatcggg tcggcggtac ggttgaccaa ttccgccggt
      661 tccccgtggc gctcttcagg aggggggtcg ggtgaggtca gccgagagcg aggtggcagg
      721 acgtatttcc agactccccc ctcgccatct tcgatcggcc ctctgggaca agcggccccc
      781 agagggccgt tgtgtctcct ggtcacatgt cggacggtga ctgcggccgg ggcagatgaa
      841 gagacgactg gaggtcagga atgaatgaga tactccttgg caagggtgag cagcctgtcc
      901 atttgctcgc caaatacagc aaccggcatg gcctgatcgc tggcgccacc ggtaccggca
      961 agaccgtctc gctcagcgtc ctggcagagg gcttctcgcg tctgggcgtg ccggtgttca
     1021 tggccgacgt gaaaggcgat gttgccggcc tcgccctggc gggtgcctcc agcgagaaga
     1081 tcaaggcccg cgttgccgaa atcggcatcg agggattcgc tcacgaggcc agtccggtgg
     1141 tgttctggga tctatacggc caggccggcc atcctgtccg ggcgacggtc agcgaaatgg
     1201 gtcccaacct gctgggtcgc atactccagc tcaacgatac ccaggccggg gtgctcgaaa
     1261 tcgccttcca gctggcggat gaccggggct tgctgttgct cgacctcgac gatcttcgcg
     1321 ctctgctcgg gttcctcgcg gacaaccacc aggagatctc gacgaagtat ggtctggtca
     1381 gcacgcaatc ggtggcagcc atccagcggg ccctgttgtc gctggagcga gagggcgggg
     1441 atgccctctt cggcgagccg gcgctggagc tgggtgacct gctccgcacc gatctcagcg
     1501 gccgcggcgt cgtcaatatc ctcgccgccg atcggctgat tttgaagccc cagctctact
     1561 cgagctttct gctgtggctg ctgtcggagt tgttcgagaa tttgcccgag gtaggcgacc
     1621 tggacaaacc caagctggtg ttcttcttcg acgaggccca cttgctgttc aacgaagccc
     1681 cgccggcgct gcgccagcaa gtggagcagg tcgtgcgaat catccggtcc aaaggcgtgg
     1741 gcgtgtactt ctgctcgcag ttcccggacg acgtgccgaa cgagattctc ggccagctcg
     1801 ggaaccgcat ccagcacgcc ctgcgcgcct acaccccgcg tgaccagaag gcggtgcgga
     1861 ccgctgccga aacatttgtt gccaatccga agctcgacgt cgccaaggtc atctcccagc
     1921 ttggggttgg tgaggcgctg gtgtccacgc tcctggagaa aggtgtgccg atgccagtcg
     1981 agcgcaccat gatgtgtccg ccgcgttgtc ggataggggc gatcacgccg gacgagcgcg
     2041 cggcggtccg ggcgcgcagc ccggtcggtg caaagtacga ctcccgggtg aatcgcgagt
     2101 cggcttacga aatattgacc cggcgaaccg tatcgccaga ggcgcccccg acatcgccgg
     2161 accgagcacg ggcggagaag ccatctggcg ccggcgggca gctgggtgaa ttgctctggg
     2221 gcaccaaggg ccgccagggc ctggtggaag ccatggcgaa gcaggctgcg cgcactgtcg
     2281 ggagccagat cggccggcag atcctccgcg gggtgctggg cggcatcctc ggcggttcgc
     2341 gcgggcgtta gtgtggcgga gaatcgtggc gggtgatggc tgtcgccgtc caagccgatg
     2401 gagctgaagc ggtcagtcgc gttcgacgcc cgccgttcat cggcaagccg tcggacggtt
     2461 agcgggatag ctgaagggag cgtgggtgtt cttcagggcc ggggccgtgt ggttcatgct
     2521 gctcgccatc gccgtcgcgg ctggagccgc gagaacgacg ctgctgcaac cgcaggtcgg
     2581 cgagcaggca gcccacgtga tcgggacgct ggccggggtg gccatcttcg cggtcgtcat
     2641 ttggcgcacg gtgccctgga tcgcgccccg ttccgatcgg gggcaactgg tccgggtcgg
     2701 aattgggtgg gtcgcggcga ctgtgctctt cgagttcggt tttgggcgct acgtgatggg
     2761 ccatccctgg tcccggctcc tggccgacta caacctcttc gctggccggt tgtgggtgct
     2821 ggttctcctg acgttgcttc tgatgccgat cgcggcagcg gagttccgag ggcgcgcttc
     2881 ccgatagcca cgaacccggc tgctacgcat accggttgct gatccgtcac catccctggc
     2941 tgcagtcgca gcactcacgc cgatggcgaa ggcaaaggga ggccaacggt gggcgcgatt
     3001 tctgaggcgc tggtagatga aacttggcag gagatcggtc gcctttcgga gacgcaggca
     3061 cggagccgca tggaccagat tgccagggcg cagccccacc tcctggcgtt tgtcctgggg
     3121 acgacaggaa acctgcgggc ggatgctcag caagtggcgg tgtacctgtt cgcggtcatt
     3181 caccgcatgt ttgagaaagc cgtcggacgg ctcacccgtg ccatgcctcc ggagatcgac
     3241 gtagtctacg agcgcaacca aagatttctc gagaagtttg agcatagcca tccgcgcttc
     3301 ttggagcagg ccgcgaagtc gatgacttcc tcgcaaccct tcgtgatgaa gtacctggtg
     3361 gaagccctga tggagactcc ggagggcgac gctcccgtgg ccctatccga ggaggaaacc
     3421 ggcctgatct tcctcgtcct caagaccgtg gtcgacatcc tggatgggct ggcacagcgg
     3481 tcatcagagg caggtgacga cacggcttga gtcaaccaag ctatcctcga acgcgccgca
     3541 actcctgcat catccgcaac gtgggcgaag ccttctcctg agatgaccac gaacgggcca
     3601 aggcttggga acaacatggt ccccgacctg cggataaatc ggacgaccag ccacgcacat
     3661 cggacttcgc gactcgaatg tcacgacgtg tgttttcttt ctctcggcat tcccgcctgg
     3721 cccgcccact ggtccaggac cttgggttgt tgattcgcaa caccagatgc tcgctgcctg
     3781 aaatccttga caggcaacat cccatctgat accacaccca ctcagcaaac cctgtccagt
     3841 cgaatgccct gcttcagcca tcgtcggtga gagatgtacc ctgcgatctg atgccccaag
     3901 gaggaccccg tgaaagctcg tgagatccac gaatacaacc agctgacctg gcgcgaaatg
     3961 aacgatgcca tcgagatgca gaaggtggtg atcctgccca ccggctcgac cgagcagcac
     4021 gggccgcacc tgcccctgga cgtggatctt ttcctggcgg cctccgtctg ccacgaactg
     4081 ggccgacgag ccccggacaa ggttctggtg ctgcctgcca tcccgttcgg tctgaacatg
     4141 caccatatcg acttcccagg aacgatccac atccagccgg atcatttcat caacttctgc
     4201 ctggacgtga ccaagagcgt gtcctaccac ggcttcaagc ggatcgccct ggtgaacggt
     4261 cacggctcga acggcccgat ggttgacctg gtggcccggc gcaccgtcct ggagaccccg
     4321 tccatttgct gcgccttcgg ctatttctcc atgggatggg aggaggttct gaagctcctg
     4381 gagagtgagg tgcatgcgca cgccgacgag atggagacgt ccctctacct gcacctcgcc
     4441 aaggagcgtg ttcggttgga ccaggcggtt ttcggcaacg accacgtcgg gcactacatg
     4501 agctcggact ccacgaagac tgtccgcttc aacgacttct gggggcgctg gacccagacc
     4561 ggcgtgcatg gcgatccgac caaggccacc gccgagaaag gaaagcagat cctggagatt
     4621 gtgatgaagc atatgctggc ctggctcgaa gagtaccgga cctggcccat cgagcctcgc
     4681 tcggatcagc accggctgcc cgtccagaag gaaattcgct ggtagtcgct cgtttcaggg
     4741 caacgccttt cgaggaagga ggaccgacca tgacacgccg tgagttgttt gcctggatcg
     4801 cggggggcat tctgggcttc ctgctcctgt tcgggttcgc gggcccggcg cccgcaggca
     4861 cggtcctgaa gatcgcggcc gacggggaca tctccgacgt cgacctacat atgacgaccc
     4921 attatctgaa ccgggtcgtt ctgctcaacg tgtacgacat gctgtttgct ctgggcgagg
     4981 acatggggcc caagccgtcc ctggtggaca agtacacggt aagctccgac ggcctggtct
     5041 acaccctgac gcttcgccgc ggcgtgaagt tccacaacgg caagaccctg gacgccaagg
     5101 atgtcgtgta ctccatcacc aagatgcaga acaagggacc gcggtccggg cagttcaaga
     5161 agcttatcaa gagcctcgag gccgccgatc cgctgaccgt gaagatcatg ctcaatgagg
     5221 tgaacgccgg cttcatcgcc acgctggcga accccatcgc cccggcggtg atctacccgg
     5281 acggcgaggc agagcgacag gggggcacga tcaccaagcc ggtgggcacg ggcccgttcg
     5341 agttcgtgga gtggaagaag gacagcgtcg tccggctgaa gaagttcgcg ggctacaccg
     5401 tggacgatcg ccccacctcc gggttcacgg ggaagcggct ggagctggtg gatcaggtgg
     5461 acttcatccc catccgggac tcctcggtgc gggcggcggc gctggagcgg ggcgacgtgg
     5521 acgtggccct ggccctcacg cagcaggacg tggaacgcta caagggcaaa ccgggcctca
     5581 cagtcgccgc caagccgggc gtctcgttcg ccgacctccg cttcgggttc aagaagggta
     5641 tcttcatgaa caaccagaag cttcgccagg cggtggggta cgccatcgac aaggaggaga
     5701 tcgtcaaggc ggtgaccttc gggttgggaa agtccgcgcc ggccgggatc cctgtcggga
     5761 ccccatactt cggcgcggtg catgccgccg atccattcgg caagcccaat cccgagaagg
     5821 ccaagcagct cctgaaagaa gccggctaca agggtgagga gatcatcctc gactcgcacc
     5881 tgcagcccag cgtctatccg gagatcgcgg tggtcctcca ggcgcagttc caggcggtag
     5941 ggctgaacgc tcgggtacgc acgctggagt cggcggcgct gcagacgaca ggggatgggg
     6001 gggagtttgc cttcttcctc tcggggctca gcccgcgccc ggatgccgag atctactact
     6061 gccagttctg ggagagcagt agcacgtcga ccggctacaa gaaccccgag tacgaccggc
     6121 tatgccgggc cgcgcgaacg acgatgaagc aggaggagcg gaccaaggtc ttcgtcgacc
     6181 tggagaccct gctccgtacc gacgtgccgt ggattccgct catctacctg ccgcaagtgg
     6241 agggctggcg cagcaacgtc caggggtggc agcactggtc cgcggggtat gcacgcgcct
     6301 ggaacgtcac ggtagggaaa tagtgccaag tgcctagtgc cgagagccta gactagagtt
     6361 gagccggggc gaggagcgtg atccgccccg gctcgatctt tcgatccgat cgcggacgtc
     6421 gca
//
LOCUS       DBNGZC010001105         1631 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1070894, whole genome shotgun sequence.
ACCESSION   DBNGZC010001105 DBNGZC010000000
VERSION     DBNGZC010001105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1070894"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="AC16G0_19100"
     CDS             complement(<1..201)
                     /locus_tag="AC16G0_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008957146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5101770.1"
                     /translation="MELSTFLQALITGILMAGIYALVGIGLTLIFGVVRIVNFAHGEF
                     VMLGMYLTFWLWKLWGMDPYLSL"
     gene            complement(292..1551)
                     /locus_tag="AC16G0_19105"
     CDS             complement(292..1551)
                     /locus_tag="AC16G0_19105"
                     /inference="COORDINATES: protein motif:HMM:NF024825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5101771.1"
                     /translation="MGRDRRFHDLVSRREFLKGATALGVGAGLAGFPVRRSHAAAAKD
                     VNIAVLYPTSGSNARFGQMCVNAIKQAVEDVNSAGGIKSLGGAKLNPTYADIQSDATV
                     TRNQAERLMAAGNLAAATGCYVSTFTLVATEVAERYKIPFVTGSVADKLTERGFKYTF
                     QVSPKASHFGAMQMDFAAKLAGKGKRAAVIYEDTDYGTATTKGVVDGAKTVGFEVVMN
                     EPYVAKFTDATPLVNKIKASKPDFLFPVSYITDALLIIRTMKQMNVNAGIFGGGAGYL
                     IPDFFKDMGKDADYVFSVASWNNDINCAEVAKIADTYEKKYGEFLMEHAGEAYVMIWV
                     IADALQRAASTDPVKVRDAMAKTNLTKGPGSVMPGCHVEFDDKGWNKHVHPVMTQWQN
                     GKLRCVAPESDARVKPVFPIPAWDKRG"
BASE COUNT          281 a          516 c          510 g          324 t
ORIGIN      
        1 gagggagaga taggggtcca tcccccagag cttccagagc cagaaggtga ggtacatccc
       61 cagcattacg aactcgccgt gggcgaagtt cacgatccgg accacgccga agatgagggt
      121 tagtccgatc cccaccaggg cgtagatccc tgccatcagg atgccggtga tgagggcttg
      181 caggaaggtc gaaagctcca ttgcacgcgc gcgggggtca agggacaccg cccctgaccc
      241 ccgcgtccta catgcgtaac cgctgccccc gagtgcgggg cgtcctcaca tttatccgcg
      301 cttgtcccag gccgggatcg gaaagaccgg cttcacccgg gcgtccgatt ccggggccac
      361 gcagcgaagc ttcccgttct gccactgggt catgaccggg tggacgtgct tgttccaccc
      421 cttgtcgtcg aactccacgt ggcagccggg catgaccgaa cccggcccct tggtcagatt
      481 ggtcttggcc atggcatccc gcaccttcac ggggtcggtc gaggccgctc gctggagggc
      541 atcggcgatg acccagatca tgacgtaggc ttcgccggca tgctccatga ggaattcacc
      601 gtacttcttc tcgtaggtgt cggcgatctt ggcaacctcg gcacaattga tgtcgttgtt
      661 ccaggacgcc accgagaaga cgtaatcagc gtccttgccc atgtccttga agaagtcggg
      721 gatgaggtag cccgcgccgc ccccgaagat gcccgcgttg acgttcatct gcttcatcgt
      781 ccggatgatg agcagggcgt cggtgatgta ggagaccggg aacaggaaat cgggcttgga
      841 tgccttgatc ttgttgacca ggggcgtggc gtcggtgaac ttggccacgt acggctcgtt
      901 catcaccact tcgaagccga ccgttttggc cccgtccacg acccctttgg tggtcgcggt
      961 gccatagtcg gtgtcttcat agatgacggc tgcccgcttg cccttccccg ccagcttggc
     1021 ggcaaagtcc atctgcatcg cgccgaagtg gctggccttg ggggagacct ggaaggtgta
     1081 cttgaagcca cgctcggtga gcttgtcggc aaccgaaccc gtgacgaacg ggatcttgta
     1141 gcgctcggcc acctcggtgg cgaccagggt gaacgtgctg acgtagcagc ccgtggccgc
     1201 cgccaggttc cccgcggcca tgaggcgttc ggcctggttc cgcgtgaccg ttgcgtcgct
     1261 ctggatgtcg gcgtatgtcg gattcagctt ggccccaccc agggacttga tcccgccggc
     1321 gctgttgacg tcctccaccg cctgcttgat ggcattcacg cacatctgac cgaagcgggc
     1381 attggaaccg gacgtgggat acaggacggc gatgttgaca tccttggctg cggcggcgtg
     1441 cgacctgcga accgggaagc cggccaggcc tgcaccaacc cccagggccg tcgccccctt
     1501 caggaactcc cgccgactga ccagatcatg gaatcgtctg tctctcccca tcgttttgtc
     1561 ctcctgagtg tgagccgctg ttgctcggat gaaaacgacc tgcatcgtat gtcagggcct
     1621 tccggaaagt c
//
LOCUS       DBNGZC010001106         2858 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_486707, whole genome shotgun sequence.
ACCESSION   DBNGZC010001106 DBNGZC010000000
VERSION     DBNGZC010001106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_486707"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="AC16G0_19110"
     CDS             <1..966
                     /locus_tag="AC16G0_19110"
                     /inference="COORDINATES: protein motif:HMM:NF013722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR5101772.1"
                     /translation="KRMKGGVSAVTGTRKGVAPRYENIVLDEDFGPITEVVDDHKVKR
                     FAFIMDDYHPWYFGESPFGQRIGHAGILANDLLQLFTQVYDANTVVGLHTHEELWFVN
                     PVRVPEQVTLYGKYVEKYEHRGKGYVVMEADARGEDGRVLLRHRGVEILHIEPGPVVG
                     KSTAQARERRVTGEYRKDVEPVSHARVGLAPGTPLVPLHKQPLQDQIAVFSLVGKHVR
                     NVHTDIEVARRAGLKDTLAQGMMESIYLTELLTSFFGPTWFTTGWQKVKFIRPVYCGD
                     RLTARGVVTGDRHESGRTRLEVDIWVENAAGEMTAVGWASGRLAE"
     gene            1421..>2858
                     /locus_tag="AC16G0_19115"
     CDS             1421..>2858
                     /locus_tag="AC16G0_19115"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR5101773.1"
                     /translation="MASLTAIGHSLQEPFDPRRFLAEFSVHLQRLMPHDRLLIVYLEE
                     GGMLSVFAEHALQGPMIHEGRYTITFDPGGRYTPAELTLGPVLAGEAMRVGDFQDDSR
                     FAGPGVDTPRAVKIGLRARLAVPLTSGRRIIGALLVGSYTPNRFTEAQLAAARQVADL
                     IAPFIENIVLLHREQRRRSRLARLVGLACVFGASLDLKGSFTHVAAAVRPILDFDVMR
                     AGLLGVSGRDLELIGAVEDHPGPAAPSRISLDDLSMAAKIQSGEPVLIRDAQEELDRA
                     HPGDRGIIADGIRACLAVPLQFCEEVGGFLFFAKRRPGWYDGSDVEIASAIASQVVVA
                     IQHQRLAEEQGRRAHIEGRARQLEQRLTTLRQELGERFGFDRILGRAPGLQEVLARAA
                     KVAATETTVLLTGESGTGTELVARAIHYSSPRADGPYMAINCAAIPETLVESELFGHE
                     RGAFTGADRQKPGRFELAAGGTLFLDEIG"
BASE COUNT          495 a          882 c          967 g          514 t
ORIGIN      
        1 aaacgtatga aagggggcgt tagcgccgtg acaggaactc gcaagggtgt agctcctcgg
       61 tatgagaaca tcgtgctgga tgaggatttc ggcccgatca ccgaggtggt ggacgatcac
      121 aaggtgaaga ggttcgcctt catcatggac gattaccatc cctggtactt tggcgagtcc
      181 ccctttgggc agcgcatcgg gcacgcaggc atcctcgcca acgacctgct ccagctcttc
      241 acacaggtct acgacgccaa tacggttgtg gggctgcaca cgcacgagga gctctggttc
      301 gtgaatccgg ttcgtgtccc cgagcaagtg accctctacg gcaaatacgt cgagaagtac
      361 gagcaccgcg gcaagggcta cgtggtgatg gaggcggatg cgcgggggga ggacggccga
      421 gtgctcttgc gccaccgagg agtggagatc ctgcacatcg agcctggccc agtcgtgggg
      481 aaaagcacgg cgcaggcgcg tgagcggcgg gtgaccggcg agtaccggaa ggacgtcgag
      541 ccggtgagcc acgccagagt cggcctggcg ccgggcacac ccctggtccc cctgcacaag
      601 caaccgcttc aagatcagat cgccgtcttc tccctggtgg ggaagcatgt ccgaaacgtg
      661 cacactgaca tcgaggtggc ccggagagcg gggctcaagg atacgctggc ccagggtatg
      721 atggaatcga tctacctcac cgagctcctc accagcttct tcggccccac ctggttcacg
      781 accggatggc agaaggtgaa gttcatccgg ccggtctatt gcggagacag actgaccgcg
      841 cgcggcgtgg tcaccggcga ccgtcatgag agcggcagga cccggctcga ggtggacatc
      901 tgggtggaga atgcggccgg cgaaatgacg gcggtaggct gggctagcgg ccgccttgcg
      961 gagtagcgaa tccgggcgta tttcgggtgg tgggcatctg cagatgggcc tgttgtcgca
     1021 gtggctatga tgtagcgata caggatgggc tgaacccgcc cccccacctc cttcctcgct
     1081 ctcttctgtg cggtgcgccc acacaggtgc gatcggccga tcccctggcc accccccagc
     1141 ggatgcgtcc gcctgattga cttcccgtca tacgcctcct atcatccccc gtacctcctg
     1201 cgccgaagga gtactcccaa catggcgagc cttcggttgc ggaaatattg agtagacaat
     1261 cgccagctcg ttcttttctc agcttgcgag agggagctgg gccaagtcgg catgacgcga
     1321 cgatgcgtct gcgcgaaaac agcgggggtc ggcgcggtcc ggcgtgacgc acacttagct
     1381 cggagacagg atggacgttg cacttgctga ccctgacctg ttggcttctc tgactgccat
     1441 cggccattcc ctacaggagc ccttcgatcc gcgacgcttt ctggcagagt tctcggtgca
     1501 tctgcagcgg ttgatgcccc acgatcgcct cctgatcgtc tatctcgaag agggcggaat
     1561 gctttctgtc tttgccgagc acgccctgca ggggccgatg atccacgagg gccgctacac
     1621 catcaccttc gaccccggcg gccgctacac acccgccgaa ttgaccttgg gtccggtgct
     1681 tgccggcgag gcgatgcggg ttggggactt ccaggatgat tcgcgttttg ccgggccggg
     1741 cgttgatacg cccagggcgg tgaagatcgg tctgcgggcc cggcttgcgg ttccactcac
     1801 cagcggccga cggatcatcg gggccctgct ggtggggagt tacaccccca acagattcac
     1861 cgaggctcag ctcgccgcgg cccggcaggt ggccgatctg atcgcgccct tcatcgaaaa
     1921 cattgtgctg ctccaccgcg agcagcggcg ccggagccgc cttgcgaggc tggtcggcct
     1981 ggcctgtgtc ttcggggcca gcctggatct caagggaagc ttcacgcatg tggcggcggc
     2041 agtccgaccc attctggact tcgatgtcat gcgggcgggc ctgctcggcg tcagcgggcg
     2101 cgatctggag ttgatagggg cggtggagga ccatccgggc ccggccgcgc cgtcgaggat
     2161 ctcgctggat gatctctcca tggcggccaa gatccagtcg ggcgaaccgg tgctgatccg
     2221 ggacgcccag gaagaactgg atcgcgcgca tccgggggat cgcggcatca ttgcagatgg
     2281 aattcgcgcg tgtctggcgg tgccgctcca gttctgcgag gaggttgggg gtttcctgtt
     2341 cttcgccaag cgccgacccg ggtggtacga cgggtccgat gtcgagattg caagcgccat
     2401 tgcgtcgcag gtcgtggtgg caattcagca ccagagactc gcggaggaac agggacgccg
     2461 cgctcacatc gaggggcggg cgcgccaact ggaacagcgt ctgaccacgc ttcggcagga
     2521 actgggagag cgattcgggt tcgaccggat ccttgggcgc gctccgggcc tccaggaggt
     2581 gctcgcccgg gcggccaagg tcgcggcgac cgagacgacc gtcctcctta cgggagagag
     2641 cgggactggg acggaactgg ttgcccgcgc catccactac agcagcccac gcgccgatgg
     2701 cccctacatg gccatcaact gcgctgccat tcccgaaaca ctcgtggagt cggagctgtt
     2761 cggccacgag cgcggggcct tcacgggggc ggatcgccag aagcccgggc gcttcgagct
     2821 ggcagctggc ggcacgctct tccttgacga gatcggcg
//
LOCUS       DBNGZC010001107         4281 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1071376, whole genome shotgun sequence.
ACCESSION   DBNGZC010001107 DBNGZC010000000
VERSION     DBNGZC010001107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1071376"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1793
                     /locus_tag="AC16G0_19120"
     CDS             <1..1793
                     /locus_tag="AC16G0_19120"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5101774.1"
                     /translation="AAPPISRAAVLSGTSAADLLNIVRTLAAPDMEGRATGSPGMQRA
                     ARYIAAEFQQTGLLPSADGGSYLQAFDVVTGIHLGDQNRLQLLRAEGSQGYQVRRDFT
                     PFSFSDSGRAQGEVAFVGYGITAPELHYDDYTGVDVAGKIVLLLSGDPRERDITGPFR
                     QPEAYRYTEVRYKVLNAREHGARGVILVTNPVAHGEEPEHLFAIRGVTSVSQSGILAI
                     NALRRVADAILAGSGHSLRELQETIDRDLRPRSFVVSALTAEIDVELIHEHGQGWNVI
                     GLLPGTDPTLREQAIVVGAHYDHLGHGGETSLAPERYGEIHPGADDNASGVAGVIGLA
                     RLLAAEGGARRTIVFIAFAGEEMGLLGSSHYVRAAAVPLEHTAAMLNMDMIGRLRDDT
                     LYVFGVDTGKEFREVLDAANRDVGLTFRLSGDGYGPSDHTPFYGKNLPVLFFFTGPHP
                     DYHRPSDTPDKINASGLSRVVQLVAGVLRRLAEGSAPVTFVRVKGSSPPRPSAERGQG
                     YGPYFGLVPEFGQPDDGVRLNGVLAGSPAEKAGLRTGDLIVRFDGRTVRNLEDFVFVL
                     RSKRAGDQVEVVYRRGSEEHTVGAVLGVRP"
     gene            1960..2178
                     /locus_tag="AC16G0_19125"
     CDS             1960..2178
                     /locus_tag="AC16G0_19125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101775.1"
                     /translation="MQREEMVVDVLTVRGWYRGFITLPAGGRLLDFLNTKPEMIALTG
                     VKDPSGVHHSFVALNTSQVLAIRPGVQA"
     gene            2213..2965
                     /locus_tag="AC16G0_19130"
     CDS             2213..2965
                     /locus_tag="AC16G0_19130"
                     /inference="COORDINATES: protein motif:HMM:NF012774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HIR5101776.1"
                     /translation="MGRAGRQDAGDPPGQWQFLRDDREIAQSMTHTSPAGSLAAIDSP
                     GRRAKLSGWSAVALLLVLACLGLTRPGGAGTPNTSKTPKPSQALPSQAPIQGQTWGTV
                     RRVESAAGLVVATPELARIEVRLLGVEPPVVIRPARDGAKPTPDQPYGGQAETYLRDL
                     LQDKQVVLDFYGKDRAGRSLAVVWLGDINVNLTLVKQGLAWISPSIPITRVRVELEVA
                     ERQAQVGKYGLWALPDPEPPWEFRKRHRLPAE"
     gene            3003..>4281
                     /locus_tag="AC16G0_19135"
     CDS             3003..>4281
                     /locus_tag="AC16G0_19135"
                     /inference="COORDINATES: protein motif:HMM:NF012492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR5101777.1"
                     /translation="MTLPTSDPSTPLPLGEDDRRFLEAFRQRYPFPLDPFQEQAIRWV
                     LSGDSVIVSAPTGAGKTLIAEFAIYRALAIQRSVVYTTPLKALSNQKYADFVRQYGND
                     RVGILTGDVKVNPRAPILVMTTEILRNLLFTEKPQEIATAVLDECHYLGDEGRGTVWE
                     EIVINCPARVQIVALSATVSNLHEIAAWISQVHRPIHAISHPVRPVPLQHLLCHQDGE
                     IVSLEAPRRARSVVPEAGYRGSRERAGRRGFAMHGRMHRRPANPVRVIPQLRERHWLP
                     AIYFIFSRSGCERALRRFLEDGGSLLETHRLPEVEEAVANTLRDYPSIVPETEVNTLL
                     LHGLRRGAGLHHAGVLPALKRLTEVLFERGLVKVVFATETMSLGIHMPAKSVVIQGVR
                     KRSEAGFRQLSLNELTQMAGRAGRRGIDPEGTCL"
BASE COUNT          723 a         1402 c         1472 g          684 t
ORIGIN      
        1 cagccgcgcc gcccatcagc agggcagcgg tcctctccgg aacctctgcc gcggacctgt
       61 tgaacatcgt gcggaccctg gcggctcccg acatggaggg ccgcgcgacc gggtcgcccg
      121 ggatgcagcg ggcggcacgg tacatcgcag ccgaattcca gcaaaccggc ctcctgccct
      181 ccgcagacgg ggggtcctat cttcaggcct tcgacgtggt cacgggcatt cacctgggcg
      241 accagaaccg cttgcagctc ctgcgtgcgg aggggagcca ggggtaccag gtcagacggg
      301 acttcacgcc gttcagcttc tccgacagcg ggcgagccca gggggaagtg gccttcgtcg
      361 gctatggcat cacggcgccc gagcttcact acgacgacta caccggcgtg gacgtcgccg
      421 gcaagatcgt cctgctgctg tcgggcgacc cccgagagcg cgacatcact ggcccgttcc
      481 ggcagcccga ggcttaccgg tacaccgagg tgcggtacaa ggtcctcaac gcacgggagc
      541 atggtgcccg cggggtcatc ctggtgacga accctgtcgc ccacggggag gagcccgagc
      601 acctcttcgc catccgggga gtcacctcgg tgtcccaaag cggtatcctg gcgatcaatg
      661 cgctgcggcg ggtggccgat gccatcctgg cagggagcgg gcattccctg cgtgagcttc
      721 aggagaccat tgaccgtgac ctgcgccctc gctcgttcgt ggtgtcggca ctcacggccg
      781 agatcgacgt ggagctgatc cacgagcacg gccaaggctg gaacgtcatc ggtctcctgc
      841 cggggaccga tcccacgctg cgggagcagg cgatcgtggt gggggcccat tacgatcacc
      901 tcggccacgg tggggagacc tccctcgcgc cagagcgcta tggggagatt catccgggag
      961 cggacgacaa cgcctcgggc gtggccgggg taatcggcct ggctcgcctc ctcgccgccg
     1021 agggcggggc gcgacggacg atcgtcttca tcgcctttgc cggtgaggag atgggtctgc
     1081 tcgggtcctc gcactacgtc cgggcggcgg cggttcccct ggagcacacg gcggccatgc
     1141 tgaacatgga catgatcggt cgcctgcggg atgacaccct ctacgtcttt ggcgtggaca
     1201 ccgggaagga gttccgggag gtgttggacg cggccaatcg ggatgtgggg cttacgttcc
     1261 ggctgagcgg agatggctac ggcccgtcgg accacacgcc gttctacggc aagaatctgc
     1321 cggtgctttt cttcttcacc gggccgcatc cggactatca tcgaccctcc gataccccgg
     1381 acaagatcaa tgcctccggc ctgtctcggg tggtacagct cgtggcggga gtcctccggc
     1441 gcctcgccga gggaagtgca ccagtcacgt tcgtccgggt gaagggctcc tcgcctcccc
     1501 ggcccagtgc cgaaagaggg cagggctatg gaccctattt cggcctggtc ccggagttcg
     1561 gacagccaga cgacggggtg cggctgaacg gcgtgctcgc cggcagcccg gcggagaagg
     1621 cgggacttcg aacgggagac ctgatcgtgc gcttcgatgg gcggacggtc cggaacctgg
     1681 aggattttgt ctttgttctc cggagcaaac gtgccggaga ccaagtggag gtcgtctatc
     1741 gacgcggaag tgaggaacac acggtcggcg ccgtgttggg ggtccggccc tgaaatcggg
     1801 tggcgggggt gaggggccgg aggtagggaa ggacgtgttc gccgttgaca ggacgagtgg
     1861 cattgggtag cctgcaggtg cgtcaagagt cgacgaggcc ccggagcggt caggcaacgg
     1921 gtccgagagc catatccgcc acaccagggg gacaggcgaa tgcagcgaga agagatggtg
     1981 gtggatgtcc tcaccgttcg tggatggtat cggggattca tcaccctgcc ggctggcggg
     2041 cggctcctgg atttcctcaa caccaagccc gagatgatcg cgctcaccgg agtaaaggac
     2101 ccctcggggg tccaccattc gtttgtggcc ctcaacacat ctcaggtctt ggccatccga
     2161 ccaggggttc aggcatagac atccagggag gagatgaacc ccgcacccct ggatgggacg
     2221 cgcgggccgc caggatgccg gagatcctcc tgggcagtgg cagtttctcc gggacgacag
     2281 agagatcgcc caatccatga cgcacacctc gcctgctggc tcgctcgccg ctattgactc
     2341 acccggccgg agggccaagc tttccggatg gagcgcagtg gcgctgctcc tggtcctggc
     2401 gtgcctcggg ctgacccgac ctggtggggc cgggactccc aacacctcca agaccccgaa
     2461 gccgagccag gcgcttccct cgcaggcacc cattcaggga cagacgtggg gcacggtccg
     2521 gagggtggag agcgccgcag gcttggtggt ggcgactccg gagcttgcgc gcatcgaagt
     2581 tcgcctcctg ggggtcgagc cacccgtcgt catacggcca gcccgcgacg gagccaagcc
     2641 cacccccgac cagccctacg gcgggcaggc ggagacctat ctccgggatc tcctgcagga
     2701 caagcaggtg gtactcgact tctacgggaa agaccgagcg ggtcgaagct tggcggtggt
     2761 ctggctcggc gacatcaacg tcaacctcac cctggtgaag caagggctgg cctggataag
     2821 cccctccatc cccatcacca gggtgcgggt ggaactggag gtggcggaac gacaggcgca
     2881 agtcggcaag tacgggctgt gggcgcttcc cgacccggag ccgccctggg agttccggaa
     2941 gcgccaccgc ctccccgccg agtgagcggc gtggcgcaca ggggaagagg aagaaggcga
     3001 gggtgaccct gccgacgtcg gacccatcca cgccgctccc cctcggagag gatgaccgcc
     3061 ggttcctgga ggccttccgg cagcgctatc cctttccgct cgacccgttc caggagcagg
     3121 ccatccgctg ggtgctgagc ggggactccg tgatcgtctc agccccgacg ggcgccggca
     3181 agaccctgat tgcggagttt gccatctacc gcgccttggc cattcaacgc tcggtcgtct
     3241 ataccacccc cctcaaggcc ctgagcaatc agaaatacgc ggacttcgtc cgccagtacg
     3301 gcaacgatcg agtgggcatc ctgacgggcg acgtgaaggt gaacccgcgg gcccccatcc
     3361 tggtcatgac cacggagatc ctgcgcaacc tcctgttcac ggagaagccg caggagatcg
     3421 ccacggcggt cctggatgag tgccactatc tcggggatga gggccgcggc accgtgtggg
     3481 aggagatcgt catcaactgc cccgccaggg tgcagatcgt ggcgctctcg gcgaccgtga
     3541 gcaacttaca cgagatcgcg gcctggatca gccaggtgca tcgccccatc cacgccatct
     3601 cccacccggt gcgacccgtc cccctgcagc atctgctttg ccatcaagat ggggagatcg
     3661 tctccctgga ggcgcccagg cgcgcccggt ccgttgttcc ggaggccggc taccgcggca
     3721 gccgggagcg ggcgggacgc cgaggattcg cgatgcacgg acggatgcat cgccgccccg
     3781 ccaatcccgt tcgagtcatc ccgcagctcc gcgagcgaca ctggctcccg gcgatctact
     3841 tcatcttcag ccgatcggga tgcgagcgag cccttcgtcg cttcctggag gacggcggct
     3901 cgctgctgga gacccaccgc cttcccgagg tcgaggaggc cgtcgccaac accctgcggg
     3961 attaccccag catcgtcccg gagacggagg tcaacactct cctgctccac gggctgcgtc
     4021 gcggagcagg cctccaccac gcaggggttc tgccggcgct caagcggttg accgaggtgc
     4081 tcttcgagcg ggggctggtg aaggtggtct tcgccaccga gaccatgagc ctggggatcc
     4141 acatgccggc caagagcgtg gtgatccagg gggtccgcaa gcggagcgag gccggcttcc
     4201 gtcagctgag cctcaacgag ctgacgcaga tggcgggccg ggcggggcgg cgcggcatcg
     4261 acccggaggg aacctgcttg a
//
LOCUS       DBNGZC010001108         3388 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2050717, whole genome shotgun sequence.
ACCESSION   DBNGZC010001108 DBNGZC010000000
VERSION     DBNGZC010001108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2050717"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1354)
                     /locus_tag="AC16G0_19140"
     CDS             complement(<1..1354)
                     /locus_tag="AC16G0_19140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101778.1"
                     /translation="MEAAPGQTFRLGTWEGALEGVMDYGRQRTKTDGQATSSFDNFRV
                     EERLRLRNVDAFILDPRLATLTFGGTFGLTQEWQKFDGTSDYTDGTLWGYEGFLRLLP
                     EAPYSLNVFANRDQDVNSLIFGGRTETVEQNFGAKLFAKRIYIPSSLTFRQEQTTQET
                     RTGDVVARQDQVRNIVTYEGSRGWENAEATLGYEFTDLTDKILPDLSFRSHDGNLTYS
                     LDFGPELNRRWDSRMRIFSRTGETSLTTGTVDELLRIDHSDRLQTNYRYFLLFNDTAS
                     GSTTTQTGDFHLHHQLYESLTTDLRLGAVGALLPGGSKQTYLSRLDFSYLKKLPGDGR
                     LHIGLGGGLQYDDNSFQTQETTVLQETHTFATPFALPIRLANPFVITESVVVTKTAVG
                     PLPAGCAPPSGPPTPLQVGRDFTLLTTASLTEIVPIPCAGATPGINPGDTIAVDYRFA
                     V"
     gene            complement(1415..1738)
                     /locus_tag="AC16G0_19145"
     CDS             complement(1415..1738)
                     /locus_tag="AC16G0_19145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR5101779.1"
                     /translation="MPRRIRIVGAILLAGLLASAALAVEGDVVLSRKGGTGGVPLAVF
                     PHWFHRIRYKCYACHDAIFKMKQGADDVTMDAITAGKFCGACHDGKTALGVTFETCNR
                     CHIER"
     gene            complement(1738..2745)
                     /locus_tag="AC16G0_19150"
     CDS             complement(1738..2745)
                     /locus_tag="AC16G0_19150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c(7)-type cytochrome triheme domain-containing
                     protein"
                     /protein_id="HIR5101780.1"
                     /translation="MPEASGGAGSARATPAPPTRRVRRDLLLENEELKRQLQEANQAL
                     KAREATPAPQETRVPAENAHAYEEAEGLLPKDAAGRVDWIQALSSGTVRPRPGITAQT
                     PEQAALDLDVELTESGNALFSATYPHRPHTQWLACGNCHPAIFPISRKVPPSVITMAK
                     IRNGEQCGVCHGKVAFPVDKECGRCHTKTPARSDWHPVEEPRTPIERATSPDEALRLL
                     PVTAGMPDWAKALADGLIAPRPGIDPKAEEQPAFPLDVELVPADNALFKVVFPHGTHT
                     ALLACTTCHPGIFQMAKGADPITMGAIYAGQYCGSCPGKVAFAVPTGCPRCHPALAGG
                     K"
     gene            complement(2895..>3388)
                     /locus_tag="AC16G0_19155"
     CDS             complement(2895..>3388)
                     /locus_tag="AC16G0_19155"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5101781.1"
                     /translation="VLRPSPSQFANPIEMARLDDGRMYAAIKLGRPGTAMGGWGELLS
                     PVEIIDVMRYVRTLAQPSATGTGAGQLDLSVGQQVYRQHCVQCHGEKGDAQTPLGQSL
                     TPHPRDFTQVREMTAISDRDLTQAIALGKPGTAMAPWRGVLNSDDIRRVVLYIRRTFQ
                     RAP"
BASE COUNT          556 a         1092 c         1112 g          628 t
ORIGIN      
        1 ggacagcgaa ccgatagtcc accgcgatcg tatccccggg gttgatcccg ggggtcgccc
       61 ccgcgcaggg gatggggacg atctccgtca ggctggccgt cgtcagcagg gtgaagtcgc
      121 gaccgacctg cagcggtgtc ggcggtcccg agggcggcgc gcagccggcc ggcagggggc
      181 ccaccgcggt cttggtgacc acgacgcttt ccgtgatcac gaagggattc gcaagccgaa
      241 tcggcagggc gaatggggtg gcgaaggtgt gggtttcctg caggacggtt gtctcctgcg
      301 tctggaagct gttgtcgtca tactgcagtc ccccgcccag cccgatgtgc agccgcccgt
      361 cgcccggaag cttcttgaga tagctgaagt ccagacggct gagataggtc tgcttgctcc
      421 ccccaggaag caatgcgccg accgccccta accggaggtc ggtcgtcaga ctctcgtaga
      481 gctggtggtg gaggtggaag tccccggttt gggtcgtggt gcttccgctc gctgtatcgt
      541 tgaacaggag gaagtaccgg tagttggtct gaagtcggtc tgaatggtca atgcggagaa
      601 gctcgtccac cgtacccgtg gtcaggctcg tttcccccgt ccggctgaag atccgcatcc
      661 gggaatccca gcgccgattc agctcgggac cgaagtccag gctatacgtg agattcccat
      721 cgtggctccg aaaactgagg tcggggagta tcttatcggt gaggtcggtg aactcgtagc
      781 ccaaggtggc ctcggcgttc tcccagccgc gcgagccctc gtaggtgacg atgttccgca
      841 cctgatcttg ccgggccacc acgtcgcccg tgcgcgtctc ttgggtagtc tgctcctggc
      901 ggaaggtgag gctggacgga atatagatcc ttttggcaaa gagcttcgcg ccgaagttct
      961 gctccacggt ctcggtccgc ccgccgaaga tcagactgtt aacgtcctgg tctcggttcg
     1021 cgaacacgtt gagggagtag ggagcctcgg ggaggaggcg gagaaaaccc tcgtatcccc
     1081 acagggttcc gtcggtgtaa tccgacgtgc cgtcgaactt ctgccattct tgggtgaggc
     1141 cgaaggttcc cccgaaggtg agcgtggcca ggcgagggtc caggatgaag gcgtccacgt
     1201 tccgcagcct gaggcgctcc tcgacccgga agttgtcaaa ggaagaggtc gcctgtccgt
     1261 cggtcttcgt ccgctggcgc ccgtagtcca tcacgccttc cagggcgccc tcccatgtcc
     1321 ccaggcgaaa ggtctggccg ggggcggcct ccacgaaaaa aaaagacgct ccaggccgcc
     1381 agcagcccgg cgcaccatcc tagcgctctg cgcctcatcg ctcgatgtga caccggttgc
     1441 aggtttcaaa ggtgacgccc aacgccgttt ttccgtcatg gcaggccccg cagaacttcc
     1501 cggcggtgat ggcgtccatg gtgacgtcgt ccgctccctg cttcatcttg aagatggcgt
     1561 catgacaggc atagcatttg tagcggatac ggtggaacca gtgcgggaag acagccagcg
     1621 gaacccctcc agtcccgccc tttcggctca agacgacatc cccttcgacc gccagcgctg
     1681 cgctggccag gagtcctgcc aagaggatcg cgcccacaat ccggatgcgg cgtggcatca
     1741 cttccccccc gccagggcgg gatgacacct cggacatccc gtggggacag cgaaggccac
     1801 cttgccgggg cagcttccac agtactgccc agcataaatc gcccccatgg tgatggggtc
     1861 tgcccccttc gccatctgga agatacctgg atggcaggtg gtgcaggcga gcaaggcggt
     1921 gtgggtccca tgcgggaaga ccaccttgaa cagcgcgttg tctgccggga ccagctcgac
     1981 gtccagcgga aaggcaggct gctcctccgc cttcgggtca atgcccgggc ggggcgcaat
     2041 cagtccgtcg gccagggcct tggcccagtc aggcatgccc gcagtgaccg gcaggagcct
     2101 gagggcctcg tccggactgg tggcccgctc gatgggcgtg cggggctcct cgaccggatg
     2161 ccaatcgctc cgagccggag tcttggtgtg gcaccggcca cattccttgt ccaccgggaa
     2221 cgccaccttt ccatggcaga ccccacactg ctccccattg cggatcttcg ccatggtgat
     2281 cacgctggga ggcaccttcc gtgagatggg gaaaatggcc ggatggcagt tgccacaggc
     2341 cagccattgg gtgtgcggtc ggtgagggta ggtcgcggag aagagcgcat ttccagattc
     2401 ggtcagctcc acgtccagat ccagcgccgc ctgctcgggc gtctgtgcag tgatacccgg
     2461 ccgcggcctg accgtgcccg agctgagcgc ctgaatccag tccacccggc ccgctgcatc
     2521 cttcggcagg agtccctccg cctcttcgta tgcgtgcgca ttctcagcgg gcacgcgcgt
     2581 ctcctggggg gccggagtcg cttccctggc cttcagcgcc tggttggcct cttggagctg
     2641 ccgcttcagc tcctcgtttt ccaagaggag atcccgccgg acgcggcggg tgggcggtgc
     2701 cggagtcgcc cgcgccgatc cggccccgcc ggatgcctca ggcacgccgt cgaagaaaac
     2761 gggcaggaga ctttgacgcg attccgcgct gcagccggta agcagcgcag ttgcgaagta
     2821 agccgccgca ccaatcgctc gcatgcggtg ccttggagtc acgaagaggt tcctctactc
     2881 tcccagtgcg ggtcttacgg cgcgcgctgg aatgttcggc ggatgtagag gacgactcgc
     2941 ctgatgtcgt cggaattcag cacgccacgc caaggcgcca tggccgtgcc cggtttcccc
     3001 agggcgatcg cctgggtcag gtccctgtcg ctgatggctg tcatctctcg gacctgggtg
     3061 aaatcgcggg gatggggagt caggctctga ccaagcggcg tctgggcgtc gcccttctcc
     3121 ccgtggcatt gcacgcagtg ctggcgatac acctgctgac ccacggagag gtcgagctgt
     3181 cccgctcctg tcccggtcgc cgaaggctgc gcgagcgtcc ggacgtatcg catcacatcg
     3241 atgatctcca ccgggctcag cagctctccc cagccaccca tggccgtgcc gggtcggccc
     3301 agcttgatgg cagcgtacat ccgtccgtcg tccagacgcg ccatctcgat cggattggcg
     3361 aactggctcg gcgacggtcg aaggactt
//
LOCUS       DBNGZC010001109         4344 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2538732, whole genome shotgun sequence.
ACCESSION   DBNGZC010001109 DBNGZC010000000
VERSION     DBNGZC010001109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2538732"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..548)
                     /locus_tag="AC16G0_19160"
     CDS             complement(60..548)
                     /locus_tag="AC16G0_19160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010548065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5101782.1"
                     /translation="MKVLRIVPDRCTGCMRCELACSYMQTGAFQPSRSVIRVSPFEGH
                     TSYAPYTCTQCAEGWCMTACPVEAISISPVGAKVVREDQCVGCKLCTIACPYGTIFYN
                     PESQKAFKCNLCGGSPACAEACPTAAIEYVEAETIDWLGAFAEGRGQRELAEAHAGVE
                     VG"
     gene            1269..1928
                     /locus_tag="AC16G0_19165"
     CDS             1269..1928
                     /locus_tag="AC16G0_19165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR5101783.1"
                     /translation="MPPAPAPDVPFDPNTLLAKIGEGRSIGDYRKNRTVFAQGDRADA
                     VFFLRKGKVKLTVVSNQGKEAVVAILDEGAFFGEGCLVAQPLRMATAITLTPCSILRL
                     EKLAMVRLLHAEPAFAERFIAHLLARNIRIEEDLVDQLFNSAEKRLARVLLLLANFGK
                     EGKPEVVIPKISQETLAEMIGTTRSRVNFFMNKFRKLGFLDYNDGLEVNSSLLNIILH
                     D"
     gene            2403..2873
                     /locus_tag="AC16G0_19170"
     CDS             2403..2873
                     /locus_tag="AC16G0_19170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5101784.1"
                     /translation="MDFGIVPRRLLVIDDDPSFRITVECLLRKLGHTVDVAESGSAGI
                     ALLRQKRVDLVMTDLSMPGLTGWDVARLVKVMHPRLPVLLVTGCAPTIPPDQPERTWV
                     DAILAKPCGVAAMQAVIGALTRDLAEGACSGGPERAGSTTVRGWPFESPLVEDA"
     gene            3012..3290
                     /locus_tag="AC16G0_19175"
     CDS             3012..3290
                     /locus_tag="AC16G0_19175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101785.1"
                     /translation="MATIVEYTDRKRPRNEYPSRIISPTRAQACCFSDMEELGTSHET
                     DRWVFQYKRCRQCGFTVRVILREIPDEVLVWTLRQILATAFQRNQPEF"
     gene            complement(3690..>4344)
                     /locus_tag="AC16G0_19180"
     CDS             complement(3690..>4344)
                     /locus_tag="AC16G0_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011385441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HIR5101786.1"
                     /translation="AWHENFILPLSHDEVVHGKGSLQQKMPGDDWQKFANLRSFFGFM
                     YGHPGKKLLFMGGEFGQTSEWYHEVSLSWHLLGMGPYHTGVQRLVQDLNRLYREEPAL
                     HQVDFEPAGFQWLDCNDWEGSVIAFLRRARDPEDFLVVVCNFTPVVRQGYRIGVPRGG
                     FSQELGNTDGAVYGGSNVGNAGRVLAEAVPKHGHNQSLRLTLPPLATLVLKPERVAA"
BASE COUNT          771 a         1440 c         1337 g          796 t
ORIGIN      
        1 ggtcaggatg gcgttcattc cggcggtccc cccgcccacg atcacgtgtc gcctggccat
       61 catccaacct ccacgccggc atgcgcttcc gccagctccc gttggcccct gccctcggcg
      121 aacgcaccca gccagtcgat cgtctccgcc tcgacatact cgatggccgc cgtgggacac
      181 gcctccgcac acgcgggact gcctccgcag aggttacact tgaaggcctt ctggctctcc
      241 gggttgtaga agatggtgcc gtacgggcag gcgatcgtgc acagcttgca cccgacgcat
      301 tgatcttccc ggaccacctt ggcccccacc ggtgagatgc tgatggcctc caccgggcac
      361 gcggtcatgc accagccttc cgcgcactgc gtgcaggtgt acggggcgta gctggtgtgc
      421 ccctcgaagg gcgagacccg gatcaccgac cggctgggct ggaaggcacc cgtctgcatg
      481 tacgagcacg ccagctcgca gcgcatgcag ccggtgcagc gatcgggaac gattcgcaac
      541 accttcatgg atacccctct gggaaaagtg agaagtgagt cgcgtggccg gggacggtgc
      601 agtcccccct catgcctgac gcgcgccgga ccgttcactg cagcgtgatt cttatagcat
      661 gccaaggccg aatcgtccaa gggcggaaga ctcgaaaacc acatcgctga attccgcgca
      721 ctgacgagcg gaaagatatc cgataccttc ctcacactcc cgtaatttcg ctgttctttt
      781 tccatcgact ccctgctatc atttgtgcat gcacaggcca gcccgcctgc gtatccgaag
      841 gtcctcaacg ggcgtccctt cctgcttgtc tcctgcagcg cccacgtcgg ggaatcttgt
      901 ctgcttggac ccgctcgaaa gccggcgccg aatcgagaga ctgatgtccg ggttctcggc
      961 gcagttcata atatttccca cgaaacaggt cgagggccgg agcgcggtcg ctgagcggac
     1021 ggttgcctta cttccatctc ttcgcgtctc caggggacgc ccgggcaatc cctgggggaa
     1081 gtcagccggc ccttctcacg caccgcatcc aacgcctggg ctctcagtca ccggccgagg
     1141 cccaggccaa ggcatggact gaggcgacat gaccgagcgt acgccttgtc cagggaaccg
     1201 ggaggagtag cgtgagtaca cccaagagtc gcaagggcca gacgctgaag cgcgtcaatg
     1261 ggagcgcgat gcctccggca cccgctccag atgtgccgtt cgacccaaat acgctccttg
     1321 ccaagatcgg ggaagggcga tcgatcgggg actaccgcaa gaaccggact gtctttgcgc
     1381 aaggagacag ggcggatgcc gtgttcttcc tccggaaagg aaaggtcaag ctgacggttg
     1441 tgtccaatca aggcaaggag gcggtcgtgg ccattctgga cgaaggcgcc ttcttcgggg
     1501 aaggctgcct ggtcgcgcag ccgctccgaa tggcgacggc catcacgttg acgccctgct
     1561 ccatcctgcg gctggaaaaa ttggccatgg tgcgcttgct ccatgccgag ccagcctttg
     1621 ctgagcggtt catcgcgcat ctgctcgccc gcaacatccg tatcgaagag gacctcgtgg
     1681 atcaactgtt caattccgcg gagaagcggc tcgcgcgggt gctgctgctg ctggccaact
     1741 tcgggaagga ggggaaaccg gaggtcgtga ttccaaagat cagccaggag accctggcgg
     1801 agatgatcgg caccacccgc tcgcgggtca acttcttcat gaacaagttc cgcaagttgg
     1861 ggttcctcga ttacaacgac gggctggagg tcaacagttc cctcctgaat atcattctac
     1921 acgactgacg gccgcccccg accggccgcg ggcacgcatc ccaccgtttt cgaccgcctg
     1981 tgcaatattg aacagatcgc ccagccaaca ctgagtactc tcctcccatt cggatcctgt
     2041 cccgattctc acacgcgatg cggaggcagg acagcctcca gcacgaggga ttgccgagta
     2101 gccgcttccg ttgcagccaa acaacatgcc gggaggtcgt ggtcgacagg gggtcccccg
     2161 gtcgcggaag tctcgatctt cgcattgcca ctcaatcgat ccagggcaca atcccagcgt
     2221 tcggggatga tcgcctccgt ctccaactga gcaatcgcga tgaagggaag gacggaccgt
     2281 cgagccaggc ggcaaccggc gaggtcccac ggctctcgat ttcccgtccc gggcccggca
     2341 gcccagtggt cgtgtttcgc atgtagggtt cccccgcgcg gaccgccacg gaggaagcca
     2401 tgatggactt cggcattgtc ccacgacgcc tcttggtcat cgacgatgat ccatcattcc
     2461 ggataacggt ggagtgtctt ctccggaaac ttggacacac ggtggatgtg gccgagagtg
     2521 ggtctgccgg tatcgcactc ctccgccaga agcgggtgga cctggtgatg acagacctca
     2581 gcatgccggg actcacgggg tgggatgtgg cccggctcgt gaaagtcatg cacccgcgcc
     2641 ttccggtgct cctcgtgacc gggtgcgcgc cgaccattcc ccccgaccag cccgagcgga
     2701 cgtgggtgga tgcgatcctg gcgaagccgt gcggggtggc agcaatgcag gcggtgattg
     2761 gcgcgctgac ccgggacctc gccgaggggg cgtgctccgg cggcccggag agggcggggt
     2821 ccacgacggt gagaggatgg ccgttcgaat ctccgctggt ggaagatgcg tagggcgcgc
     2881 cggcggacgt gcccggcatt tccataccgc cgttcctccc gaccgccgag cacggacgga
     2941 gaccgctgac cgcatgccta ctctctaggc agcaagttca ttggcaccat ccgagtcagg
     3001 aggggcggcc catggccacg atcgtcgagt acacggatcg gaagaggcct cggaatgagt
     3061 atccgagccg catcatctcc cccacccgtg cccaggcctg ctgtttttcg gacatggagg
     3121 agctcgggac gtctcacgag acggaccgct gggtctttca gtacaagcgc tgccggcaat
     3181 gcggcttcac ggtccgggtg attctgcggg aaattcccga cgaagtcctg gtgtggacgt
     3241 tgcgtcagat tctggccaca gccttccaac ggaaccagcc ggaattctaa taggggtgcg
     3301 cggccggagg ggttgcagac cacacccctg ccccgaaccg cgtcatcggc tggggtctcc
     3361 ttcggccctt ccccctccca acgatcgctc ttgctcggac cgtttgatcc acgcctcaat
     3421 ctcctcggcg gcaatcttct gcgcagccag gaccatgatg gcgcgcctgg tttcctctct
     3481 catttcggcc tcggggaccg ccagctctcg agccgcctcg cgcgcacggt ttacccaggg
     3541 ctcatgaggt tggacttcac gatctcccgc ttcctctgac atcgcaccct ccttgctccc
     3601 ctcctcaccc cagatttacg gcctggcctt cgcccaggag ggggccgaac caggcccagt
     3661 tgttgaggta ggaatcgtcc ggcacgtcgt caggcggcaa cccgctccgg cttgagcacc
     3721 agggtcgcca gcgggggaag ggtcaggcgc agggactggt tgtgcccgtg cttggggacg
     3781 gcctcggcca ggacgcgccc ggcgttgccc acgttgctcc cgccgtagac cgcgccatcc
     3841 gtgttcccca gttcttgaga gaacccgcct cggggcacgc cgatccggta gccttggcgg
     3901 accaccggcg tgaagttgca gacgacaacc aggaagtcct cggggtcccg cgcgcgcctc
     3961 aggaaggcga tcacgcttcc ctcccagtcg ttgcagtcga gccactggaa gcccgcgggc
     4021 tcgaaatcca cctggtgcag ggcgggctct tcccggtaca ggcgattcag atcctggacc
     4081 agtcgttgca cccccgtgtg gtacggcccc atccccagca ggtgccagct cagactgacc
     4141 tcgtggtacc actcgctcgt ctggccgaat tccccgccca tgaacaagag cttcttgccc
     4201 gggtggccgt acatgaagcc gaagaagctt cgcaggttgg cgaacttctg ccaatcgtcc
     4261 cccggcatct tctgctggag cgagcccttc ccgtggacca cctcgtcgtg ggatagggga
     4321 aggatgaagt tctcgtgcca cgcg
//
LOCUS       DBNGZC010001110         2335 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1562691, whole genome shotgun sequence.
ACCESSION   DBNGZC010001110 DBNGZC010000000
VERSION     DBNGZC010001110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1562691"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="AC16G0_19185"
     CDS             complement(<1..1179)
                     /locus_tag="AC16G0_19185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR5101787.1"
                     /translation="MKSYRLLALLWLLALTAFHLWLIGSGRWPLAPDEAHYWEWSRRL
                     DWSYYSKGPMVAYLIALSTRLGGDTEFWVRLPAVLLGTGTAAIGYLLARRIFQSERAG
                     FLCVVLLSLMPLYAAGSFLMTIDPPFVFCWGLASLCLFQILQHRGEMAWYQVGVALGL
                     GLLSKYTMLMLLPCVLLWLLLSPRLRPWLRRREPYEAAVLGLLIFSPVIIWNARHRWL
                     SGRHVLIQAGAGGERGLGPSLLGGPEFLGTQLGVISPFLFVLLLLAAAWAWRQGLRRG
                     RDDLLLLACLSLPVFLFFQVWSFVTKVQANWAAHAYFTAAVAAAGWRESWVDWGEHRR
                     GTRRLNGLLLASILLPAVVLPVSFFPDLLGVFGARVPAAADLVSKRLRGWPELGVAVG
                     E"
     gene            complement(1295..1816)
                     /gene="purE"
                     /locus_tag="AC16G0_19190"
     CDS             complement(1295..1816)
                     /gene="purE"
                     /locus_tag="AC16G0_19190"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR5101788.1"
                     /translation="MASKTSAAAPLVGILMGSDSDLPTMQEAVKILQDFGIPLEVRIC
                     SAHRTPDRLAGYARQAEGRGLRVLIAGAGGAAHLAGVLAAHVTVPVIGVPMEAGPLHG
                     LDALLSTVQMPGGIPVATVAIGRAGARNAALLAVQILALSDRRLQRKLRTYRARMAAE
                     VAAKDQKVQAEQA"
     gene            complement(1851..>2335)
                     /locus_tag="AC16G0_19195"
     CDS             complement(1851..>2335)
                     /locus_tag="AC16G0_19195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012531883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR5101789.1"
                     /translation="AGLMIMDGNHSVLEFYVRFGDPEAQPLLVRMESDLLPVLEAVLD
                     RRLHELEIAWSPSAAVCVVMVAGGYPGAYDQGKVITGLPAAARLKDVVIFHAGTSLHG
                     GKVVTSGGRVLGVTALGRDISEAIPRAYRAVEKIRWDGVHYRTDIGRKAINRPTAQGS
                     "
BASE COUNT          454 a          781 c          784 g          316 t
ORIGIN      
        1 ctcgccgacc gccaccccca gttccggcca cccgcgcagc cgcttgctga cgaggtcggc
       61 ggcggccggg accctggccc cgaagacccc gaggaggtcg ggaaagaagc tcacgggaag
      121 caccacggcc ggcaggagta tagaggcaag caagagacca ttcaagcgtc tcgttcctcg
      181 acgatgctcc ccccaatcca cccagctctc acgccagccg gccgctgcca ccgccgcggt
      241 gaagtaggcg tgggccgccc agttggcttg caccttggtg acgaagctcc agacctgaaa
      301 gaacaggaac accgggagcg acaagcacgc caggaggagc aggtcgtcgc gccctcgccg
      361 caatccttga cgccaggccc aggccgcggc gagcagcagg aggacaaaca ggaacgggga
      421 gatgactccc aattgtgtgc cgaggaactc gggtcctccc aggagggacg ggcccaggcc
      481 tcgctcaccg cctgccccgg cctggatcag gacatgtcga cccgagagcc agcggtggcg
      541 agcgttccag atgatcaccg ggctgaagat caagagcccc aagacggccg cctcataggg
      601 ctcgcggcga cggagccacg ggcgaagccg gggcgagagg agcaaccaga ggagcacgca
      661 gggcaggagc atcagcatgg tgtacttgct gagaaggccg agccccaagg cgacgccgac
      721 ctggtaccag gccatctccc cccggtgctg gaggatttgg aaaagacaca ggctggctag
      781 accccagcag aagacaaagg gtgggtcgat ggtcatgagg aaggacccgg cggcgtacag
      841 gggcatcagg ctgagaagaa ccacgcagag aaatcccgcg cgctcgctct ggaagattcg
      901 acgcgccagc agatagccga tggccgcggt gccggttccc aggaggaccg ccggaagccg
      961 gacccagaac tccgtgtcgc cgcccagcct ggtgctgagg gcgatgaggt aggcgaccat
     1021 ggggcccttg ctgtaatagc tccagtcgag tcggcgggac cactcccaat agtgagcctc
     1081 gtcgggcgcc agcggccagc ggccgctccc gatgagccac aggtgaaacg ccgtgagcgc
     1141 cagcagccac aggagggcga ggaggcggta ggatttcacg cggccggccc ctcgctctgt
     1201 gctgcctgtg tcatgcatgc ggcgcgcggg tggcgcgctt gaggagagag aggtgagggg
     1261 tggagcagga catgcggtcc ccgggaaggt gccatcaagc ctgctcggcc tggaccttct
     1321 ggtccttggc ggcgacctcg gccgccatgc gggcgcgata ggtgcggagt tttcgctgga
     1381 gacgacgatc gctgagcgcc aggatctgca ctgccagcaa ggccgcgttc ctggcgccgg
     1441 cccgcccaat ggcgacggtg gcgacgggaa ttccgccggg catctgcacc gtggacagca
     1501 gggcatccag accgtgcaag gggccggcct ccatcggcac accgatgacg ggaacggtca
     1561 cgtgggccgc cagcacgccc gccagatggg ccgccccccc cgccccggcg atcaggacac
     1621 ggaggccccg tccttccgcc tggcgcgcgt aaccggcgag ccgatcggga gtccggtgag
     1681 ccgagcagat ccgcacctcc agcggaatcc caaagtcctg gaggatcttc accgcctcct
     1741 gcatcgtggg cagatccgaa tcgctgccca tcaggatccc cacgagggga gccgcagccg
     1801 aagtcttcga tgccatggta tctccggagt gctcggctat cggtcaggca ctacgagccc
     1861 tgagccgtcg ggcggttgat ggcctttcgg ccgatgtcgg tccggtagtg gaccccgtcc
     1921 cagcggatct tctccacggc acggtaggcc cgggggatcg cctccgagat atcccttccc
     1981 agagcagtca ctcccagcac gcggccgcca ctggtcacca ccttgcctcc atggagggac
     2041 gttccggcgt ggaaaataac cacgtccttg aggcgggccg cagccggcag ccccgtgatc
     2101 accttcccct ggtcatatgc gccgggatag cctcccgcca ccatgaccac acagacggca
     2161 gcactgggag accaagcgat ctccagttcg tgcagccgcc gatccaagac ggcctccagg
     2221 acgggtaaca gatcgctctc catccggacg aggaggggct gggcctcggg atctccgaaa
     2281 cggacgtaga actccagcac gctgtgattt ccatccatga tcatgagacc ggcat
//
LOCUS       DBNGZC010001111         1551 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2248516, whole genome shotgun sequence.
ACCESSION   DBNGZC010001111 DBNGZC010000000
VERSION     DBNGZC010001111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2248516"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="AC16G0_19200"
     CDS             complement(<1..254)
                     /locus_tag="AC16G0_19200"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HIR5101790.1"
                     /translation="MRALTGVRVLDLSRFVAGPFCCQILGDHGADVIKIEKSDGEPAR
                     RMPPFFKDESLYFLAYNGSKRSVTLNLRSQAGLSLLRRLIA"
     gene            616..1551
                     /locus_tag="AC16G0_19205"
                     /pseudo
     CDS             616..1551
                     /locus_tag="AC16G0_19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008931874.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
BASE COUNT          294 a          511 c          490 g          256 t
ORIGIN      
        1 gcgatcaagc gtcggagcag gctcaggccg gcctgcgagc ggagattcag cgtgacgctg
       61 cgcttgcttc cgttataggc gaggaagtac aagctctcgt ccttgaaaaa agggggcatc
      121 cgacgcgccg gctcgccgtc ggacttctct atcttgatga cgtccgcccc gtggtcgccg
      181 agtatctggc agcaaaacgg ccccgccaca aaccgcgaga ggtcaaggac ccgaacgccg
      241 gtcaacgccc gcatgccaga acctccttgg agtgtcgagt ggacccgggg attggcggag
      301 acgaggccgg caggttcccc agggcgaccg agagcgtcca gatacgtttg ctcgaagctc
      361 agcacgcacc cctaacatag accctctggg tatgtcaaag ccatttcgct gcggaggcgg
      421 gcaggtcgtg aatcggcttg accggaccac ccttggttat ggcggttgac acccaagggt
      481 acctccgata cccttccggc cgtggcgtca gcgccagacc ggcacgaaac tgtcggaggc
      541 tgccgtcgcc acgcaggcgg gaggggcaac ttcctccgca ggggaggcaa ccacgcagaa
      601 cagggcacgg aagtcatgac gacccggcgc ttacagaacg tgaacgttgc cgcgttcgat
      661 ctgatgccgt ctccccagga gatccagacg cgggttccga tctcggaggc cgctgcgaac
      721 tcggtcttcg cggggcgcga gacgatcatg cgcatcctgg accgcaccga cccccggctc
      781 ttggtcatcg ttggcccttg ctcgatccac gatctcgccg ccggctatga ctacgcccaa
      841 cgactccagc gcctggcggc ggaggtccag gagacgctgg tcctggtcat gcgggtctac
      901 ttcgtcaagc cacgaaccgc gaccggctgg aaagggttca tcaacgaccc ctacctggac
      961 gactccttcc gcatcgagga ggggatggag aaggcccggg gcttcctgct gcgggtggcg
     1021 gagctgggcc tgcccatcgc cacggaagcg ctggattcca tcaccccgca atacctcggc
     1081 gatctcatcg cctggaccgc catcggcgcc cgcaccgcat agtcccaaac ccaccgagag
     1141 ctggcgagtg gcttgtccac cccggtgggg ttcaagaacg ctacggatgg cgatatccag
     1201 gtggcaatca atgccatccg cgcggctgcc cgaccccaca gtttcctcgg cctgagcgcc
     1261 caggggcgct caaccatcgt acggacgcga gggaacgccc attgtcacct ggtccttcgg
     1321 ggcggcgatg agcgaccgaa ctacgacacg gtgagcattt ccatcgtgga gcgggcgctg
     1381 gccaacgcag gcctcccgcg gattgtcgtc gtggattgtt cccacgccaa ttcccacaag
     1441 gatccggagc tacagcccct ggtgctgtcc gattgtgtgc accagatccg ggaaggcaac
     1501 cgctccatcg tcggcctgat ggtcgagagc aacatcgagg ggggcaatca g
//
LOCUS       DBNGZC010001112         2929 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_684035, whole genome shotgun sequence.
ACCESSION   DBNGZC010001112 DBNGZC010000000
VERSION     DBNGZC010001112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_684035"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..853)
                     /gene="pssA"
                     /locus_tag="AC16G0_19210"
     CDS             complement(53..853)
                     /gene="pssA"
                     /locus_tag="AC16G0_19210"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HIR5101791.1"
                     /translation="MKRNRGAMRRRMRRGVYLLPSMLTLGNLSCGFYALIAVYNDEYT
                     AAAIAILLAEVFDYLDGGVARMTGATSDIGIELDSLADLVSFGVAPGLLAYVFALKPF
                     GWIGGLAAFAFAACGAFRLARFNVQTRVLDKRFFVGLPIPAAAAAVATFVLFMKESSA
                     VVAFGRELVPAQATSAGVLLGMYGLGFLMVSRIRYRSLKGLEMRRRRPFTVLTTLLLV
                     LLIVASHPSFFLFLFFFGYAASGIVRLVPAWQKRHPVETTDQPATGKQ"
     gene            complement(1008..1637)
                     /locus_tag="AC16G0_19215"
     CDS             complement(1008..1637)
                     /locus_tag="AC16G0_19215"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HIR5101792.1"
                     /translation="MPIERDGMVFILPTLVVSGLLFAFRFPSTATAVLLLAAFLVYFF
                     RDPERVIPQWERLILAPADGRIVVIKPFPDWKGPFGEPLVRVSIFLSVLDVHVNRAPL
                     SAVVNGVTHTPGRFVAAWGETASAENEQTLIHFASPDGDVWVKQIAGLLARRIVCRLQ
                     PGQKIDAGDRFGLIRFGSRVDCIFPAAAEIKVRRGQMVKGGSTILGVIP"
     gene            complement(1759..>2929)
                     /locus_tag="AC16G0_19220"
     CDS             complement(1759..>2929)
                     /locus_tag="AC16G0_19220"
                     /inference="COORDINATES: protein motif:HMM:NF014342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HIR5101793.1"
                     /translation="DVLLNRTPFYPEAGGQVGDVGTLTADGTLVEVLDTRRPVPTLVL
                     HSVRVKRGRLTSGQTVQAVVDGSRRRLTAKNHTATHLLHAALRQVLGDHVKQAGSLVA
                     PDRLRFDFSHFSPLTSREMDHIEELVNEQVWGNRSLEIEVMDLDRALALGAMALFGEK
                     YGERVRVVKVPDFSVELCGGIHVGATGEIGLFKIASPGGVGSGVRRIEAFTGPGAFRH
                     VKLEEQVLSQAAERIKARPMELPEKVEKLTGAARDLEREVQRLQGKLAVGELDRLVSQ
                     RRDVDGIQVVAAAVELLDSKGLRELGDRVREALQSGVVVLVTQTGGRVTWLSMVTKDL
                     TSRVHAGRLAQTLAKLTGGGGGGRPDMAEAGGKDPSRIPEALEKIPALIRTQLGA"
BASE COUNT          557 a         1044 c          868 g          460 t
ORIGIN      
        1 gatcaggatc cgttcgccca tgatggacct cctccagccg atcagccggg cctcactgct
       61 tgccggtcgc gggctgatcg gtcgtctcga cgggatgtcg cttctgccac gccggcacca
      121 gacgcacgat gccggaggct gcatacccaa agaagaacag gaacaggaag aagctggggt
      181 gcgacgccac gatcaacagg accagcagca gcgtggtgag cacggtgaag gggcgccgcc
      241 gtcgcatctc caatcccttc aggctgcggt agcggatccg actcaccatg aggaacccca
      301 gaccgtacat ccccaacaag actccggcgg aagtcgcctg ggccgggacc agctcccgcc
      361 cgaaggcaac caccgccgag gactccttca tgaacagcac gaacgtcgcc accgccgcgg
      421 ccgccgccgg gatgggaagg ccgacgaaga agcgcttgtc caggacgcgc gtctgcacgt
      481 taaaccgggc gagccggaac gccccgcagg ccgcgaaggc gaaggccgcc aggcccccga
      541 tccacccgaa gggcttcagg gcaaacacat aggccaagag ccctggagcc accccgaatg
      601 agaccaagtc ggccagggag tccagctcga ttccgatgtc cgacgtggcg cccgtcatcc
      661 gggccacacc tccgtccagg tagtcaaaga cctccgccag caagatggcg atggctgcag
      721 ccgtgtattc gtcgttgtac acggcgatca aggcgtagaa gccgcacgaa agattcccca
      781 gcgtcagcat gctcgggagt aagtagacgc ctcgtcgcat ccgccgtcgc atcgcgcctc
      841 ggtttcgttt catccgaatc ctccggccgc ttcaggcccg cgaagcgtga gaggccactt
      901 gagtacaaaa aactggaacc cggcaactga tcgtcaattc accagtggcc ggaaccgttg
      961 accggctctc ggcggtcagt tctgggtgga gcttccgccc tccagcctca cgggatcacg
     1021 ccaagaatcg tgctcccccc cttgaccatc tggccgcgcc gtaccttgat ctcggccgcg
     1081 gccgggaaga tgcaatccac gcgggagccg aatcgaatca agccgaaccg atcgcccgca
     1141 tcgatcttct gccccggctg caaccggcag acaatgcggc gagccaggag cccggcaatt
     1201 tgctttaccc agacatcccc atccggggaa gcgaaatgga tcagagtctg ctcattctcg
     1261 gctgaggcgg tctcgcccca ggccgccacg aagcgccccg gggtgtgggt caccccgttc
     1321 accactgccg agagcggcgc ccggttcacg tgaacatcca gcaccgagag aaagatgctc
     1381 acccggacca gcggttcgcc gaatggtccc ttccagtccg ggaagggctt aatgacaacg
     1441 atccgtccgt ccgccggtgc caggatcagc cgctcccact gagggatcac gcgctcaggg
     1501 tcacggaaga agtagaccaa gaaggccgcc aggaggagca ccgcggttgc ggttgacgga
     1561 aagcggaatg cgaacagcag gccgctcacc accagggtgg gcagaatgaa gaccatcccg
     1621 tcccgctcga tgggcaaccg aaatcccctg gctaagtcat accctggcaa gtatagccgc
     1681 catcgactgc acaatccaac aaaaacctga tccttccccc agtcctgcgc ggattcgcct
     1741 tccgcgcccg tctgctgcct aggctccgag ttgcgtccgg atcagcgccg gaatcttctc
     1801 cagggcctcg gggatgcggg aaggatcctt gcccccggct tctgccatgt cgggtcggcc
     1861 ccctccgccc cctccggtca gcttggccag ggtctgcgcc agcctgcccg catgcacccg
     1921 ggaagtaagg tccttggtga ccatggagag ccacgtcacg cgcccgcccg tctgcgtgac
     1981 cagcaccacg acgccgcttt gcagggcttc ccgcacccgg tcgcccagct cgcgcaaccc
     2041 cttcgaatcc agaagctcca ccgcagccgc caccacctgg atcccgtcaa cgtctctccg
     2101 ctgcgatacg agccgatcca attcccccac ggcaagcttg ccctgcaggc gctggacctc
     2161 gcgctctaga tcccgcgccg ccccggtcag tttttccacc ttctccggca gctccatggg
     2221 gcgagccttg attcgctcgg ccgcctggct gaggacctgt tcctcaagtt tcacgtgacg
     2281 gaaggcgcct ggaccggtga aggcctcgat ccgccggaca cccgagccca cgccgcccgg
     2341 gctggcgatc ttgaagaggc cgatctcgcc cgtcgccccc acgtgaatcc cgccgcacag
     2401 ctcgacgctg aagtccggca ccttcacgac gcggacacgt tcaccgtact tttcgccgaa
     2461 gagggccatg gccccgaggg ccagggcccg gtccagatcc atgacctcga tctcgaggct
     2521 ccggtttccc cagacctgct cgttcaccaa ctcctcgata tggtccatct cgcgactggt
     2581 cagggggctg aagtggctga agtcgaagcg caggcggtcc ggagcgacca gcgatccggc
     2641 ctgcttcacg tgatctccca ggacttggcg cagcgcggcg tgcaagaggt gggtggccgt
     2701 gtgattcttg gccgtcagcc tccgacggct gccatccacc acagcctgga ctgtctgacc
     2761 cgaggtgagc cgaccccgct tcacccggac gctgtgcaga accagtgtcg ggaccggtcg
     2821 tcgggtgtcc aggacttcga ccagcgtccc atccgcggtc agcgtgccca cgtctcccac
     2881 ctgaccgccg gcctcggggt aaaagggcgt gcgattcaac aggacgtcg
//
LOCUS       DBNGZC010001113         3072 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1367726, whole genome shotgun sequence.
ACCESSION   DBNGZC010001113 DBNGZC010000000
VERSION     DBNGZC010001113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1367726"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /gene="hisIE"
                     /locus_tag="AC16G0_19225"
     CDS             complement(<1..591)
                     /gene="hisIE"
                     /locus_tag="AC16G0_19225"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HIR5101794.1"
                     /translation="MDVTVPQDLRFDASGLIPAIAQDAENGQVLMHAYMDAEALARTL
                     ETGLAHYYSRSRGRLWRKGEESGHVQHVRAILYDCDQDALLLKVDQAVAACHTGNRSC
                     FYRELSVGEGRGVRAEDRRFDPTVVYSGLGILLDVYGVIQDRKIHPPEGSYVATLFAK
                     GSDQILKKVAEESAEVLLAAKAGDRKQIVYEMADLWF"
     gene            complement(658..1140)
                     /locus_tag="AC16G0_19230"
     CDS             complement(658..1140)
                     /locus_tag="AC16G0_19230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016873252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HIR5101795.1"
                     /translation="MEFQTVAIQTPEGANIILGQSHFIKTVEDLHEILVSAVPGGKFG
                     IAFCESSGPCLIRSAGNDEGLREVAVKNAQAVAAGHFFVLVLKDAYPINVLNAVKQCQ
                     EVCAIYCASANPIEVVVAITAQGRGVMGVVDGLPPKGVEGSADILARKDFLRTIGYKL
                     "
     gene            complement(1164..2000)
                     /gene="hisF"
                     /locus_tag="AC16G0_19235"
     CDS             complement(1164..2000)
                     /gene="hisF"
                     /locus_tag="AC16G0_19235"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR5101796.1"
                     /translation="MLAKRIIPCLDVKEGRVVKGTRFVDLRDAGDPVGVAALYDQQGA
                     DELIFLDITASYERRRIMIDVVARTADQAFMPLTVGGGIRSLEDIRDLLKAGADKVSL
                     NTAAVREPELIRQSAERFGSQCIVLAIDAKRSAERRALSAEDESRRYQLGARSSWEVY
                     VHGGRTPTGKDVVAWAVEGERLGAGEILLTSMDRDGTKDGYDLELTRAVAEAVGIPVI
                     ASGGAGTLEHLYQGLTAGKADAVLAASMFHYREVSIPAAKEYLAGRGVRVRLQAGGPG
                     LG"
     gene            complement(2000..2731)
                     /gene="hisA"
                     /locus_tag="AC16G0_19240"
     CDS             complement(2000..2731)
                     /gene="hisA"
                     /locus_tag="AC16G0_19240"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HIR5101797.1"
                     /translation="MGIEIIPAIDLRDGRVVRLIQGDPARETRYSQEPAAVAEEWERR
                     GAPRLHLVDLDGALGGSPANQQALEAILRVIRIPAEVGGGLRSLEAIRAVLDLGAAVA
                     VLGTAAIRDPGFLEVACTAFPGRVALGLDTRGGMLAVSGWTEATRIRATDLAYQVSDL
                     PLAAIIHTDIRRDGTLEGPDLEGLHAVASTTRHPVIASGGIASTKDIQALKTLEPHGL
                     AGAIIGKALYDGRLTLEAALAAGRA"
     gene            complement(2749..>3072)
                     /gene="hisH"
                     /locus_tag="AC16G0_19245"
     CDS             complement(2749..>3072)
                     /gene="hisH"
                     /locus_tag="AC16G0_19245"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR5101798.1"
                     /translation="VRRFADRMSDPEENGVRLKVPHMGWNRVRIDRPAPILADLPDEP
                     YFYFVHSYYVEPADPTVIAGSTQHGIWFTSVIWRDNLFATQFHPEKSQATGLQLLKNF
                     AALAR"
BASE COUNT          518 a         1113 c          931 g          510 t
ORIGIN      
        1 gaaccagagg tcggccatct cgtagacgat ctgcttgcga tctcccgcct tggccgccag
       61 caagacctct gccgactcct ccgccacctt cttcaggatc tggtccgatc ccttggcaaa
      121 cagggtcgcc acataggacc cctctggggg atggatcttc cggtcctgga tcacgccgta
      181 cacatccagc aggatcccca ggccagagta cacgacggta gggtcgaagc ggcgatcctc
      241 cgccctcaca ccgcgcccct ccccaacaga aagctcccgg tagaagcagg accggttgcc
      301 cgtatggcag gccgccaccg cctgatccac cttcaggagc agggcgtcct gatcgcaatc
      361 gtacaggatc gcccggacgt gctgcacgtg acccgactcc tcgcctttgc gccacagcct
      421 ccccctggag cggctgtagt aatgcgccag gccggtctcg agggttcgcg ccaaggcctc
      481 cgcatccatg taggcgtgca tgagaacctg tccgttctct gcgtcctggg caatcgcggg
      541 tatcaacccg ctcgcatcga atcgaagatc ctgggggacc gtcacgtcca cgcttcgcct
      601 ccgccgatcc ttctgatccg cccgccagcc ggaagagacg cggcggccgg tgggacctca
      661 gagcttatag ccgatcgtcc gcaagaagtc cttccgggcc aggatgtccg cggagccctc
      721 gacacccttg gggggcaggc cgtccacgac acccatcacc ccccggccct gagccgtgat
      781 ggcgacgacg acctcgattg ggttcgccga ggcacagtaa atggcacaga cctcctgaca
      841 ctgcttcacg gcgttcagga cattgatggg ataggcgtcc ttcagcacca ggacgaagaa
      901 atggccggcg gcgaccgcct gggcattctt gacggcgacc tcgcggagac cctcgtcgtt
      961 acccgcggat cggatcaggc acggaccgga ggactcgcag aaggcgatgc cgaatttccc
     1021 gcctggcacc gcggagacca ggatctcatg caggtcttcg accgtcttga tgaaatggct
     1081 ctgcccgagg atgatgttcg ccccttcggg agtctggatc gccaccgttt ggaattccat
     1141 ggtcgccctc ctggtgtccc tgctcaaccc aatcccggcc cgcctgcctg aagtcggacg
     1201 cgcacgcccc ggcccgccag gtattccttc gccgctggaa tcgagacctc ccggtagtgg
     1261 aacatggatg ccgccaggac tgcgtcggcc ttccccgcgg tgagaccttg atacagatgc
     1321 tccagagtcc cagccccacc cgaggcgatg accgggatcc cgaccgcctc ggcaacggcg
     1381 cgggtcagct ccagatcgta gccgtccttc gtgccgtcgc gatccatgct ggtcagcaag
     1441 atctcgcccg cgccgagcct ctcaccctcc accgcccagg ccacgacatc ctttccggtg
     1501 ggcgtccgcc caccgtgcac gtacacctcc caggagctcc gagcgcctag ctgatagcgc
     1561 cgagactcgt cctcagcgct cagcgctcgg cgctcggcgc tccgcttggc gtcgatcgcc
     1621 agcacgatgc actggctacc gaaccgctcg gcggattgcc ggatcagctc gggctcccgc
     1681 accgctgcgg tgttcagcga gaccttgtcg gccccggcct tcagcagatc ccggatgtcc
     1741 tccagggacc ggatcccccc cccaaccgtc agcggcatga aggcctgatc ggctgtgcgc
     1801 gccaccacgt cgatcatgat gcgccgacgc tcgtaggatg ccgtgatgtc caggaagatc
     1861 agctcgtcgg cgccctgctg atcatacagg gcggccaccc ccactggatc cccggcgtcc
     1921 cgcaggtcga cgaaccgcgt gcccttgacg acccgcccct ccttcacgtc gaggcagggg
     1981 atgatccgtt tcgccagcat caggcccgac ccgcagccag ggccgcctcc agcgtcaggc
     2041 gaccgtcgta gagcgccttg ccaatgatcg ccccggcgag accgtgaggc tccagggtct
     2101 tcaaggcctg gatgtccttg gtagaggcga tgccgcccga ggcgatcacc gggtgtcgcg
     2161 tcgtcgatgc cacggcgtgc agcccctcca ggtccggccc ctccagggtt ccgtcccgac
     2221 gaatgtccgt gtggatgatg gccgccaggg gaaggtcgct cacctggtac gccaagtccg
     2281 tcgcccggat cctcgtcgcc tcggtccagc cggacaccgc cagcatcccg ccccgggtgt
     2341 ccaggccgag ggccacgcgg ccgggaaagg ccgtgcaggc gacctccaga aacccggggt
     2401 cacggatggc tgcagttccg aggacggcca cggccgcccc aaggtccaga acggctcgga
     2461 tcgcctccaa gctgcggagc ccgccgccga cctcagccgg gatccggatc actcgcaaga
     2521 tagcctccag agcctgctgg ttggctggcg atccccccag ggccccgtca aggtccacca
     2581 gatgcaggcg gggcgcgccg cgccgctccc actcctccgc cacggcggcc ggctcctggc
     2641 tgtaccgagt ctcgcgcgct ggatccccct ggatcagccg gacgactcgt ccgtcgcgaa
     2701 gatcgatggc gggtataatc tctatgccca cgcgaccccg tttgcccgct agcgcgccag
     2761 ggcggcgaag ttcttcagga gttgaagacc ggtcgcctgg ctcttctccg gatggaattg
     2821 tgtggcgaag aggttgtcgc gccagatcac cgacgtgaac cagattccat gctgcgtgga
     2881 cccagctatg accgtcggat ccgcaggctc cacgtaatag gagtggacga agtagaagta
     2941 gggctcgtca ggcaagtcgg ccaggatggg ggcgggccgg tcgattcgga cgcgattcca
     3001 ccccatgtga ggcaccttca accggacccc gttctcctcg gggtccgaca tcctgtccgc
     3061 aaaccgtcgc ac
//
LOCUS       DBNGZC010001114         8959 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1367849, whole genome shotgun sequence.
ACCESSION   DBNGZC010001114 DBNGZC010000000
VERSION     DBNGZC010001114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 8959)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8959)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8959
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1367849"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1877
                     /locus_tag="AC16G0_19250"
     CDS             <1..1877
                     /locus_tag="AC16G0_19250"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5101799.1"
                     /translation="WLWDEAEQLLTPGAWHGRGEWMRGRRLKLGEGLAGTVAERRAGL
                     LVNDYPTSPHAHPLVVEQTDTTAMLAEPLLYRGRLLGVISADNQGTGRTFTEKDRQTL
                     GLFANQVAIAIENARLFHESQRLAQENVLRLRTISILNEIGMAMQGTMNLDALLQVIL
                     TGATFGEGLGFNRAILLLVDEARNLLEGRMGVGPSSGEEAAQIWGALTSETRTLRDVI
                     AERAKQLGRGADTPFDRLARSLVIPLRADAGALALTALEGRPLRITNARHDPRVTPQW
                     EGRLDVDEFACVPLVAKGKVVGVIAVDNKFNRRPITDQDLEFLSVFAAQAGLAVGSAQ
                     VYTRLEDASREIQRSQHRLLQQERLAALGEMAAHVVHEIRNPLVSIGGFARRLAQRLR
                     GREPEGQYAQIIAREVDRLERIVRDVGGLSRQVNLSLVDTDVHELLQECLILFAERIA
                     HQRIQVRMNLVERPPVLALDPVQVKQAVLNLLTNALEAMPDGGTLTLGTQIVPGDQGS
                     VEDQTIGQSAAEAVAAEGQESTRRPTAEPTERPGADGWVAISVGDTGGGIPQEILDEV
                     FNPFFTTKDAGTGLGLTLVRRVARAHGGRVEVDNRPGEQVTFRLCLPMGDVGRTPPAF
                     "
     gene            1959..2336
                     /locus_tag="AC16G0_19255"
     CDS             1959..2336
                     /locus_tag="AC16G0_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008731809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5101800.1"
                     /translation="MEERATSMREILVVDDEEDIRLLYQEELQEAGYRVSVAADGAEA
                     LRMVKQSRPDLMTIDIKMPEMDGIELLRRVREIHRDLPIIICTAYGHFRQDFGTWASD
                     AYLTKSSDLMELKEKIRELLGGP"
     gene            2554..2892
                     /locus_tag="AC16G0_19260"
     CDS             2554..2892
                     /locus_tag="AC16G0_19260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR5101801.1"
                     /translation="MKKIEAIIKPFKLDEVKNALAEVGIQGITVSEVKGFGRQKGHTE
                     LYRGAEYTIDFLPKVKVEIVVPEDKCTQVVETIQASAKTGRIGDGKIFILPCEEVIRI
                     RTGERGEAAI"
     gene            3006..4418
                     /gene="glnA"
                     /locus_tag="AC16G0_19265"
     CDS             3006..4418
                     /gene="glnA"
                     /locus_tag="AC16G0_19265"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR5101802.1"
                     /translation="MTPKEVMKFSKDHGARMVDLKFMDYPGMWQHFTVPISQLEEGSF
                     EEGFGFDGSSIRGWQAIHASDMLVIPDATSAVMDPFTQTPTLSLICNIVDPITKEKYT
                     RDPRNIAQKAEAYLKSTGIADTAYFGPEAEFFIFDDIRYDSQQNCGYYFIDSVEGQWN
                     TGRAEGPNLGYKARYKEGYFPVPPTDSMADIRSEMVLTMEQIGIQIEAQHHEVATAGQ
                     AEIDMRFNTLTKIADSLMWYKYILKNVARKHGKTVTLMPKPLFGDNGTGMHTHQSLWK
                     GGKPLFAGNGYAGLSDMALWYIGGILKHAAALCALVAPTTNSYKRLVPGFEAPVNLAY
                     SSRNRSAAIRIPMYSPSPKAKRVEVRFPDCSSNGYLAFSAMLMAGLDGVQNKIDPGQP
                     LDKDIYELGPEELKDVPSVPGSLDAALLALENDHEFMLKGDVFTQDAIDTWIWYKRTK
                     EVDPVRLRPHPYEFVLYYDI"
     gene            4646..5332
                     /locus_tag="AC16G0_19270"
     CDS             4646..5332
                     /locus_tag="AC16G0_19270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR5101803.1"
                     /translation="MTESIRDRLAAVQERIGAAASRVGRDATSVRLVAVSKMMPVDVI
                     RRALDAGVTTLGENRVQEANAKISLLPGLATWHLVGHLQTNKAKLAVQLFEMIHSLDS
                     LKLAQDLDKHGRQLNTVVRCLVEVNLGSEESKSGITEEEVRALLEGAKLLPNLRIEGL
                     MAIPPFIPDAEGVRPFFRRLRNVRDKLEGEGWRLPELSMGMTHDFEVAIEEGATLVRI
                     GTAIFGARPA"
     gene            5429..6079
                     /gene="fsa"
                     /locus_tag="AC16G0_19275"
     CDS             5429..6079
                     /gene="fsa"
                     /locus_tag="AC16G0_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HIR5101804.1"
                     /translation="MQIFLDTADVKEIRELSSLGLVDGVTTNPSLVAKEGRAFREIVD
                     EIIRIVDGPISLEVVSTDAQGMLAEGRDLAKIHANVVVKIPLIAEGLKAVRQLTLEGI
                     RTNVTLCFSPSQAILAAKAGAAYISPFVGRLDDISHVGMDLVRQIVAIYKNYGYPTKV
                     LAASLRSPLHFVDAAMAGAHVCTMPPAVLRQLLKHPLTDSGLERFLADWKKVPKLS"
     gene            6617..7423
                     /locus_tag="AC16G0_19280"
     CDS             6617..7423
                     /locus_tag="AC16G0_19280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR5101805.1"
                     /translation="MEHRRKDKSEYVVQAVDRALDVLEAFNYQQEELGVTELSNKLML
                     HKNKVFRLLASLETRGYVEQDQKTGFYRLGLKTFEVANVFLHHLGLRRQARPVLEELV
                     GKCNETAYLAMTDGVDVVYVLMHETTHTVRVIPRLGFRLPAYCTASGKVQLAYESQDR
                     LQQLLSQGPLRQLTANTITDHDRLREHLAQVSQQGFAVDNEELEEGVRCVAAPVRDYS
                     HRVVAGVGLSGPVSRFGLDRIRDEFVPLVKEAAVKISQRLGYEIVPEDVN"
     gene            7595..>8959
                     /locus_tag="AC16G0_19285"
     CDS             7595..>8959
                     /locus_tag="AC16G0_19285"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR5101806.1"
                     /translation="MPEIKSSDIPVLPWPAVPRPTPDEVYKGLSEAAREYPSWLEANV
                     HWRDVGKKPEKLNRLRVLDCSIKMNIGHWCSSLFAEHGAEVIMVEPPGGDPTRKHTPF
                     GREHWMFKDGVTGEAVGGKFLHECRNKLSITLNLETPEGRELLKKLVVDADVLIENYP
                     PGQFDAWGIGYRQLSQINPRLVYCWVGQRGQWGPLKDRPWMYDPVAQNACGFTHGTGA
                     PVAFGGRPTRSGFWVCDHVGGTAAGMGIMAALNYRERISGRGQFVEATAAAGLIRIID
                     YNWGWQGMDGSVRPRFGNWDLAINIYAVNPCKDGQIMVGGGHDRLWFRIWKTVGKDRP
                     ELEQHIIEDPALKIVVDRLPYYAQVETYTTLSEWFKDNTRLEAETKLQAEEVASGGVT
                     FTDEVAEFPHFKYRGHVNEIDDALYGKVLHAPPPQLAEKAPPRLKWIGRPTGYDNEEV
                     YRRIF"
BASE COUNT         1738 a         2682 c         2983 g         1556 t
ORIGIN      
        1 tctggctgtg ggacgaggcg gagcagttgc tgacgccggg cgcatggcac gggcgggggg
       61 agtggatgcg agggcggcgc ctcaagctgg gcgaggggct cgccggtact gtcgccgagc
      121 ggcgcgcagg cctcctggtg aacgattacc cgacctcgcc tcacgcccac ccgctggtgg
      181 tggagcagac cgacaccacc gccatgctgg cggagccctt gctgtaccga ggccgactcc
      241 tgggcgtgat ctccgcggac aaccagggga cgggaaggac gttcacggag aaggaccgtc
      301 agacgcttgg tctcttcgcc aaccaggtgg ccatcgccat cgagaatgcc cggctgttcc
      361 acgagagcca gcgcctggcg caggaaaacg tgctgcggct gcgcacgatc tccatcctga
      421 acgaaatcgg aatggccatg cagggcacca tgaacttgga tgccttgctg caggtgatcc
      481 tcaccggcgc aaccttcggc gagggcctgg ggttcaaccg ggccatcctg ctgctggtgg
      541 acgaggcgcg gaacctcctg gaggggcgca tgggggtggg tcccagttcg ggagaggagg
      601 cggcgcagat ctggggcgcg ctcacgtcgg agacccgcac cctgcgggat gtcattgccg
      661 agcgtgccaa gcagctcggt cggggagcgg acaccccgtt cgacaggctg gcgcggagtc
      721 tggtgatccc gctgcgagcc gacgcgggcg ctctggcgct cacggcgctt gagggacggc
      781 cgctccggat caccaatgcg cggcacgatc cccgggtcac tccccagtgg gagggcaggc
      841 tggatgtgga cgagttcgcc tgcgtccccc tggtggccaa gggcaaggtc gtgggcgtca
      901 tcgcggtgga caacaagttc aaccggcggc ccatcaccga ccaggacctg gagttcctgt
      961 cggtcttcgc cgctcaggcg ggactggccg taggcagcgc ccaggtctat acgcgtctgg
     1021 aggacgcgag ccgggaaatc cagcggagcc aacatcgcct cttgcagcag gagcggctgg
     1081 cggccctggg cgagatggcg gcccacgtgg tccatgaaat ccggaacccc ctggtctcca
     1141 tcgggggctt cgcgcggcgg ctcgctcagc ggctgcgagg tcgcgaaccc gaggggcagt
     1201 atgcgcagat catcgcccgg gaggtcgacc ggctcgaacg gatcgtccgg gacgtcggcg
     1261 gcctctcacg ccaggtgaac ctcagcctgg ttgacaccga cgtgcacgag ctgctgcagg
     1321 aatgcctgat cctcttcgcc gagcggatcg cccaccagcg catccaggtc cggatgaacc
     1381 tggtggagcg gccgccggtc ctggccctgg accccgtgca ggtgaagcag gccgtcttga
     1441 acttgctgac gaacgcgctg gaggccatgc cggatggagg caccctgacc ctcggcacgc
     1501 agatcgtgcc gggggaccaa gggtcggtgg aggatcaaac aatcggccaa tcggcagcgg
     1561 aggctgtggc ggcggagggc caggagtcaa cgcggcgacc caccgccgag ccgaccgagc
     1621 gaccgggggc ggacgggtgg gtggcgatca gtgtggggga caccggcggg ggcatcccgc
     1681 aggagatcct ggacgaggtg ttcaatccgt tcttcacgac caaggacgcc gggaccggcc
     1741 tgggactgac gctggtgcgc cgggttgccc gcgcccacgg aggccgcgtg gaggtggaca
     1801 accgccccgg ggagcaagtc accttccgcc tctgccttcc catgggagac gtggggcgaa
     1861 ctccccctgc gttttgagcc gaggcgttgc agaggggaat gcgacctttc ggctccgaag
     1921 gagtccggcg cgagcggccg catgattggg aggcatccat ggaagaacga gccacgagca
     1981 tgagagagat cctggtggtg gatgatgaag aggacatccg cctcctgtac caagaagagc
     2041 tgcaggaggc agggtatcga gtgagcgtgg ccgccgacgg cgccgaggcg ctgcggatgg
     2101 tcaaacagtc gcggccagat ctcatgacca tcgatatcaa gatgcccgag atggatggga
     2161 tcgagttgct gcgacgcgtg cgcgagatcc atcgggacct gccgatcatc atctgcacag
     2221 cctacggcca cttccggcag gacttcggga cttgggccag cgatgcatac ctcaccaagt
     2281 cctccgacct catggaactg aaggagaaga tccgcgagct actgggtgga ccctgacatg
     2341 gtgaaaaaag tggcaggatt ccaccgggat gctcaggcgg gtggcagagg atcagtcccg
     2401 cgcgctgacg ggattccccg cgagatcgag caaatcccag tccggtgagc ggttggctga
     2461 cccggatcgc tggccccaag gcgggggttg cttggcatag ggattgctgc cctcatagcc
     2521 gggttctccg gacatcaggg cgggaggaga cggatgaaaa aaatcgaagc catcatcaag
     2581 ccgttcaagc tggacgaagt gaagaacgcc ctggcggagg ttggcatcca gggaatcacg
     2641 gtcagcgagg tcaaggggtt cggccggcaa aagggccaca cggagttgta ccggggcgcc
     2701 gaatacacca ttgatttcct gcccaaggtc aaggtggaga ttgtagtccc ggaagacaag
     2761 tgcactcagg tggtggagac cattcaggca tcggccaaga ccggtcgcat cggggacggg
     2821 aagatcttca tcctgccgtg cgaggaggtc atccggattc gcaccgggga acgcggcgag
     2881 gcggcaattt agaagcggct cccgatcccc cggtggcgga tccgggggga aggggaacag
     2941 acatctcggg accccggccg atggcctcga gcctacggcc tctccaggag gaggagcgag
     3001 aaaccatgac gccgaaagaa gtcatgaagt tttccaagga ccatggcgca cggatggtgg
     3061 acctcaagtt catggactat cccgggatgt ggcagcactt caccgtaccg atctctcagc
     3121 tcgaggaagg ctccttcgag gaggggttcg gattcgacgg cagttcgatc cgcggctggc
     3181 aggcgatcca tgccagcgac atgctggtga tccccgacgc caccagcgcc gtcatggacc
     3241 ccttcacgca gactccgacc ctgagcctca tctgcaatat cgtcgatccc atcaccaagg
     3301 agaaatacac ccgggacccg cgcaacatcg cccagaaggc ggaggcctac ctgaagtcca
     3361 ccggcatcgc cgacaccgcg tacttcggac ccgaagcgga gttcttcatc ttcgacgaca
     3421 tccgctacga ctcgcagcag aactgtggct actacttcat cgactcggtc gagggccagt
     3481 ggaatacggg ccgagccgag ggaccgaacc ttggctacaa ggcccggtac aaggaggggt
     3541 acttcccggt gccccccacc gacagcatgg ccgacatccg gtccgagatg gtcttgacca
     3601 tggagcagat cggcatccaa atcgaggcac agcaccacga ggtggccacg gccgggcagg
     3661 ccgagatcga tatgcggttc aacaccctga cgaagatcgc cgacagtctc atgtggtaca
     3721 agtacattct caagaacgtg gcccggaagc acgggaaaac cgtcaccctc atgcccaagc
     3781 cgctcttcgg cgataacggg accggcatgc acacgcacca gagcctctgg aagggcggga
     3841 agccgctgtt tgccggcaac ggctacgctg ggctgtcgga catggccctc tggtatatcg
     3901 gcgggatcct caagcacgcc gcggcgctgt gcgccttggt ggcgcccacc accaactcgt
     3961 acaagcgtct ggtgccgggc ttcgaggcac cggtgaatct ggcctactcc agccggaatc
     4021 gcagcgccgc catccgcatc ccgatgtact cgcccagccc caaggccaag cgggtggagg
     4081 tgcgcttccc ggattgcagc agcaacgggt acctcgcctt ctcggccatg ttgatggccg
     4141 ggctggacgg tgtccagaac aagatcgatc caggtcagcc cctggacaag gacatctacg
     4201 aattgggtcc agaagagctg aaggatgtgc cctcggtgcc gggctccctg gatgccgccc
     4261 tgcttgccct ggagaacgac cacgagttca tgctgaaagg ggatgtgttc acccaggatg
     4321 ccatcgacac ctggatctgg tacaagcgga caaaggaagt ggatccggtc cgcctgcgtc
     4381 cccaccccta cgagttcgtg ttgtactacg acatctagag cggcctgtcc tgtcatgggt
     4441 cagtgccccg cggcatccag cgtcgcgggg cgcttttttt cgcacgcatg gaggaaacgg
     4501 tcgccccgag cagcctgcct ggaggagatg tcgatgaggc gtgagggtct ggggatcctc
     4561 gggtctgggg ttggagtgag accgggcgct tcgttgacac catccgatag ctctgctagc
     4621 gtacggtgca tgcggaggcg ctgtcgtgac tgagagcatc cgtgaccgtc tggccgccgt
     4681 tcaagagcgg atcggcgccg ccgcgtcccg cgtgggcagg gacgccacgt cggtcagact
     4741 ggtggctgtg agcaagatga tgccggtaga cgtgatccgc cgggcgctag acgcgggggt
     4801 cacgaccctc ggggagaacc gggtccaaga ggcaaacgca aagatctccc tgcttccggg
     4861 cttggccacc tggcacctgg tggggcacct ccagacaaac aaggccaagc tggcggtgca
     4921 gcttttcgag atgatccact ccctggattc cttgaaactg gcccaggatc tggacaagca
     4981 tggtcgccag ttgaacacgg tcgtgcgctg cctggtcgag gtgaatctcg gcagtgagga
     5041 aagcaagagc ggcatcacag aagaggaggt ccgggccctc ctcgagggcg caaagctctt
     5101 gcccaacctt cggatcgaag gcttgatggc cattccccca tttatccccg atgccgaggg
     5161 tgtgcgtccc ttcttccgcc gtctgcgaaa cgtgcgagac aaactggagg gagagggttg
     5221 gcggcttccc gaattgtcga tggggatgac tcacgatttc gaggtggcga ttgaagaggg
     5281 ggcgaccctg gtgcggatcg ggacggcgat cttcggtgct cgacccgctt gacccacccg
     5341 acagggttct gcggctgccc cggtgctggc agcaacgatt gttttccctg tcggatctcc
     5401 ctgttagcgt cggacaagga ggccgatcat gcagatattt ctggacaccg ccgatgtgaa
     5461 ggagatccgg gaactgagta gccttggttt ggtggacggc gtcaccacca acccttcgct
     5521 cgtggcgaag gagggacggg ccttccgcga gatcgtggac gagatcatcc gaatcgtgga
     5581 cggcccaatc agccttgaag tggtgagcac cgacgcccaa ggcatgctgg ccgaagggcg
     5641 ggacctggcc aagatccatg cgaacgtcgt ggtgaagatt cccctcatcg ccgagggctt
     5701 gaaggcggtg aggcagctca cgctggaagg gatccggacg aacgtgacct tgtgcttctc
     5761 gccgagccag gccatcctcg cggccaaggc cggtgcggcc tacatcagcc cattcgtggg
     5821 ccgactcgac gatattagcc atgtcggaat ggacctggtg cgccagatcg tggcgatcta
     5881 taagaattac ggctatccga cgaaggtctt ggccgcgagt ctccgaagtc cccttcattt
     5941 cgtggacgcg gccatggcgg gtgcgcacgt gtgtaccatg cccccggcgg tgctccgtca
     6001 actcctgaag cacccgttga ccgacagtgg gctagagcga ttcctggccg actggaagaa
     6061 ggtccccaag ctctcctgac acgctcgccg tcgcgttttt ccgccccgcg cctatggctt
     6121 gtgcgcctga acgagaggcc tgcgtcttgg tgtgtcttct cctgcaggcc ccagctcagc
     6181 gcgcgagcat tttacttttt ctctcgatac aagcagggtg gaggaaagca tcctataagg
     6241 ctaaccagat gcatttgggt tcactaatac ttttaaaaca accattgaca actcggcaac
     6301 gacgcgtgcc agtccgctga agcttgtaaa tggtcaaaat gattacaata agaaaactgc
     6361 atgaaaccac tcgtacccct atgctgggat gctagtgcag acttgtctga gaattttgtc
     6421 tatttttgca aactcagttt tttgcttgac accgctagaa cgttatttta tatcctattg
     6481 ctcattcagt cttctaatct ggaaatcccg ataattcacg cgccgatctc ccaacagaat
     6541 ccttcgaatg cccgcctggg cgtgggccgc ggagcgattc gaatcggccc tcatgtcctt
     6601 ctttggagcg ctctgaatgg aacaccggcg aaaagataag tccgagtacg tggtccaggc
     6661 cgtggatcgg gccttggacg tcctcgaggc cttcaactac cagcaagagg aactgggggt
     6721 caccgagctt tccaacaaat tgatgttaca caagaacaag gtcttccgct tgctggcctc
     6781 cctggagact cgaggctacg tcgagcagga tcagaagaca gggttctatc gcctgggtct
     6841 caagaccttc gaagtggcca acgtcttcct gcatcacctc ggcctgcggc gacaggcgag
     6901 acctgtcctg gaggaattgg tcggcaagtg caacgagacg gcttacctgg ccatgaccga
     6961 tggtgtggac gtcgtgtatg tcctgatgca cgagaccacc cacacggtac gggtgattcc
     7021 tcgattaggc tttcgcctgc ccgcctactg caccgcctcg ggtaaggtgc agctcgccta
     7081 cgaatcgcag gatcgcctgc agcaactcct ttcgcagggt cccctgcgcc agctcaccgc
     7141 gaacacgatc acggaccatg atcggctgcg agagcacctc gcccaggtct cgcagcaagg
     7201 atttgctgtg gacaacgagg agctcgagga gggggtccgc tgcgtggctg cacccgtgcg
     7261 ggattattcc caccgggttg tggcgggggt ggggctgtcg ggccctgtct cccggttcgg
     7321 tctggaccgt atccgcgacg agttcgttcc cctggtgaaa gaggcagcgg tgaagatctc
     7381 gcagcggctc gggtatgaaa ttgttccgga ggatgtgaac tgacgctgcg gtcgtcgcgg
     7441 cgtgtcgttg caccgggcag cacgcaggtc catgcatggc attccggccc aaggggagtg
     7501 gccgccagga aaggaggtgg gaggggggag cgagggttcc tgggagaaac gatttggagt
     7561 gcggcaatgt cctactcaag ggggggaaaa cagcatgcca gagatcaaga gttccgacat
     7621 ccccgtgttg ccgtggcccg cggtgcccag gccgacgccc gatgaggtgt acaagggcct
     7681 aagcgaggca gcgcgggagt accccagttg gctggaggcc aatgtccact ggcgcgatgt
     7741 gggcaagaag ccggagaagc tcaatcgcct gcgggtcctc gactgctcga tcaagatgaa
     7801 catcggtcac tggtgctcgt cgctcttcgc ggagcatggg gccgaggtca tcatggtgga
     7861 gcctcccggg ggggatccca cccggaaaca cacccccttc ggccgcgagc actggatgtt
     7921 caaggacggc gtgaccggcg aggcggtggg aggcaaattc cttcacgagt gccggaacaa
     7981 gctctcgatc acgctgaacc tggagactcc cgagggccgc gagctgctga agaagttggt
     8041 ggtggacgcg gatgtcctga tcgagaacta cccgcccgga cagttcgacg cctgggggat
     8101 cggctaccgg cagctctccc agatcaaccc gcgcctggtc tattgctggg tggggcagcg
     8161 ggggcagtgg gggccgttga aggaccgccc ctggatgtat gatccagtcg cccagaacgc
     8221 ctgtggcttc acccacggca cgggtgcccc ggtggccttc ggcggccggc ccacccggtc
     8281 tggcttctgg gtgtgcgacc atgtcggcgg aaccgcggcc ggcatgggga tcatggccgc
     8341 cctgaactac cgcgagcgga tctcgggtcg cggccagttc gtggaggcca cggcagcggc
     8401 tggcctcatc cggatcattg actacaactg gggctggcag gggatggatg ggagcgtccg
     8461 gccccgcttc gggaactggg acctggcgat caacatctac gccgtcaacc cctgcaagga
     8521 cggtcagatc atggtgggcg gcggtcacga tcgcctttgg ttccgaatct ggaagaccgt
     8581 ggggaaggac cggccggagc tggagcaaca tatcatcgag gatccggccc tcaagatcgt
     8641 ggtggaccgc ctcccctact atgcccaggt cgagacctac acgaccttgt ccgagtggtt
     8701 caaggataac acccgcctcg aggccgagac caagctccag gccgaagagg tggcgtccgg
     8761 cggcgtcacc ttcaccgacg aggtggcgga gttcccgcac ttcaagtacc ggggccatgt
     8821 gaacgagatc gacgacgcgc tgtacggcaa ggttctgcac gcgccgccgc cccaattggc
     8881 ggagaaggcc ccgccgcggc tgaagtggat cggccggccg accggctacg acaatgaaga
     8941 ggtctaccgc cgcatcttc
//
LOCUS       DBNGZC010001115         2071 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_487858, whole genome shotgun sequence.
ACCESSION   DBNGZC010001115 DBNGZC010000000
VERSION     DBNGZC010001115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_487858"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1742)
                     /locus_tag="AC16G0_19290"
     CDS             complement(<1..1742)
                     /locus_tag="AC16G0_19290"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5101807.1"
                     /translation="MHGPNVHIDDEVLGKAYDTRLALRLLAYLRPSAVLVCLAFLFLT
                     GYTGAQLLGPYLVKVAIDRYVAAKDLAGLNRVALAYVGTIVLGFVFHFAYSYTTQVVG
                     QRVMHDIRRQLFGHLQGHDLAFFDKNPVGRLMTRVINDVENLNELLSSGVVAFLGDSL
                     MILGIAGAMFLLDWRLALVGLSLLPGIILATHAYREKARETYRESRRILSRLNGYLNE
                     NISGMATVQAFGQERRTYLKFKDINTKNRDALLRSIYYNALFFPLVEVGSAATVALIL
                     WRGGGMLLGGVLLPGVVVAFIQYVQRMYQPIRDLAEKYNIFQAAMASSERIFRLLDEP
                     ARVLCPAEPRQPQIVRGGIEFRNVWLSYLPGEPVLRDISFRVEPGEKIALVGPTGHGK
                     TSIISALCRFYDVERGQILVDGVDIREWDKTSLRKYIGLVLQDVYLFSGTIGENISLG
                     SPWITPDEIQTAARRVHASRFIKRLPHGYEEEVQERGSTLSQGERQLLSFARALAFDP
                     TILILDEATSSVDTETELLIQEALRVLLQDRTAIIVAHRLSTIQFVDRILVIHKGRIR
                     EEGTHGELLARQGL"
     gene            complement(1745..>2071)
                     /locus_tag="AC16G0_19295"
     CDS             complement(1745..>2071)
                     /locus_tag="AC16G0_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5101808.1"
                     /translation="QRTALARALVKNPPILILDDAFSSVDVQTEERILNELRTFMLGR
                     TTILISHRISTVRDADQIVYLKDGRIVEQGTHEELLAREGLYHRLYQRQLLASELEAL
                     AERENG"
BASE COUNT          381 a          659 c          681 g          350 t
ORIGIN      
        1 tagaggcctt gcctggccag gagctctccg tgggtgccct cctctcggat tcgccccttg
       61 tggatgacca ggatccgatc cacgaactga atcgtggaga ggcgatgggc cacgatgatg
      121 gccgttcggt cctgaagcag gacccggagc gcctcctgga tgagcaactc cgtctccgtg
      181 tccacggaac tcgtggcctc atccaggatc aggatggtgg ggtcgaacgc cagggcccgg
      241 gcgaaggaga ggagttggcg ctcaccctgg gaaagggtgg agccgcgctc ctgcacctcc
      301 tcctcatacc catgcggcag ccgtttgatg aagcggctag catggacccg gcgggccgcc
      361 gtctggatct cgtccggggt gatccaggga ctgcccaggc tgatgttctc gccgatggtc
      421 ccagagaaca gatacacatc ctgtaacacc aaaccgatat atttccgcaa actggtcttg
      481 tcccactccc ggatgtccac cccgtccacc aggatctgtc cccgctccac atcgtagaag
      541 cggcagagcg cagaaatgat ggacgtcttg ccgtgtcccg tgggacccac cagggcgatc
      601 ttctcgcccg gctccacgcg aaatgagatg tcgcgcagca ccggctcccc ggggagatag
      661 gagagccaga cgttccggaa ctcgatcccg ccccgcacga tctgcggctg gcgcggctcg
      721 gctgggcaga ggacccgagc gggctcatcc agcagacgaa agatccgctc gctgctcgcc
      781 atagccgcct ggaagatgtt gtacttctcg gccaggtcgc ggatgggctg gtacattcgc
      841 tgcacatact ggatgaaggc caccaccaca cccggcagca ggactccccc cagcagcatc
      901 ccgccgcctc gccacaggat gagcgccacg gtcgcggcgc tgcccacctc caccaaaggg
      961 aagaagagag cgttgtagta gatgctccgc agcagggcat cccgattctt ggtgttgatg
     1021 tccttgaact tgagataggt ccgccgctcc tggccgaagg cctggaccgt ggccatgccc
     1081 gagatgttct cgttcagata gccgttcaag cgggagagga tacgccggct ctcccgatag
     1141 gtctcccggg ctttctcccg gtacgcgtgg gtggccagga tgatcccggg gaggaggctc
     1201 agccccacca gggccagccg ccagtcgagc aggaacatgg cgccggcgat gccaaggatc
     1261 atcagactgt cgccgaggaa ggccacgacg ccggaactca agagctcgtt caggttctcg
     1321 acatcattga tcacccgggt catgagccgg cccacgggat tcttgtcgaa gaaggcgagg
     1381 tcgtggccct ggaggtgtcc gaagagctgg cggcggatgt cgtgcatcac ccgctgaccg
     1441 acgacctggg tggtgtagga gtaggcgaag tggaacacga accccaggac gatcgtcccc
     1501 acgtacgcca gggcgacgcg gttcaagccg gcgaggtctt tcgcggcgac gtaccggtcg
     1561 atggcgacct tcaccaggta gggcccgagg agctgggccc ccgtgtaccc cgtgaggaac
     1621 aggaaggcca ggcagaccag gacggcggac ggccggaggt aggcgagcag gcggagggcg
     1681 agccgggtat cgtacgcctt gcccagcacc tcgtcatcga tgtggacgtt cggcccgtgc
     1741 atggctagcc gttctcccgc tcggccaggg cctccagctc gctggccaag agctgccgct
     1801 ggtagagccg atgatacaac ccctcgcgcg ccaggagttc ctcgtgggtg ccctgctcca
     1861 cgatccggcc gtccttcaga tagacgatct ggtccgcgtc ccgcacggtg gagatgcggt
     1921 gagagatgag gatcgtcgtc cgccccagca tgaaggtccg cagctcgttc aggatgcgct
     1981 cctcagtctg gacgtccacg ctgctgaagg catcgtccag gatcaggatg ggggggttct
     2041 tgaccagggc gcgggccagg gctgtgcgct g
//
LOCUS       DBNGZC010001116         2110 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2051572, whole genome shotgun sequence.
ACCESSION   DBNGZC010001116 DBNGZC010000000
VERSION     DBNGZC010001116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2051572"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1628
                     /locus_tag="AC16G0_19300"
     CDS             33..1628
                     /locus_tag="AC16G0_19300"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5101809.1"
                     /translation="MKTKLALVVTLAMVCAVVIPSLAHAAGTLRIAQNTSDLRSMDPH
                     FATTTEDRSLVDMIFNGLVRYKPGDGSVFEPDLATSLPTPRMEGGKQVWAFQLRKGVM
                     CHPMSGVPSYELTSEDVLYSLQKSADKNRSAYAADYTGMTFEAPDASTLKISLEKPLS
                     PILFFAKVANYSGGFIVCKKAAEKLGPDALKTHPVGTGPFMFKSYSPQERVQLVANPA
                     YFRGKPQLDGVDYRFMPDLSSRELALRNGQMDVINGAPDKAWVDKMKGVSGVKVDVFG
                     VGEVASLYFNTNAQPLNKAEARKAIAYALDRDEFLALVGPGIAENVYSPVPVKFLAGG
                     LTRQELAAKGLDYKNDLAKAKKLLADAGYPNGFSLEVLTSEMTGYKVLYESMQAQLAK
                     AGIKLNVKLVDHSSFHSMIRKDANPLVIYIAWRPNADVFLTQFLHSDSIVVTGKKPNT
                     NFSHYDKIDGLIEQARNEIDPAKQIALWKEAQIKALEDMVVYPIQYQNQVYARSTAVD
                     FGHDLISVLALYPGIDEKTRLTK"
     gene            1706..>2110
                     /locus_tag="AC16G0_19305"
     CDS             1706..>2110
                     /locus_tag="AC16G0_19305"
                     /inference="COORDINATES: protein motif:HMM:NF040248.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5101810.1"
                     /translation="MFKYIAQRLVLSIPVFLGVATIVFLVVRVIPGDPAVAALGDYAS
                     KEAVEALRERMGLNASLPVQYVRFLGGLVHGDLGKSMITGTSIRADILHALPYTIELT
                     VLAIIIGTLLGIPIGVYTAIRRNRMADYQWRVQ"
BASE COUNT          413 a          673 c          639 g          385 t
ORIGIN      
        1 tcgttccaag gcctcacaag gggaggaaac ctatgaaaac caaactcgcg ctggtagtga
       61 ccctggcaat ggtgtgtgca gttgtcatcc catctctggc ccacgccgct ggcacgctcc
      121 gcatcgccca gaacacctcg gatctccgga gcatggatcc ccacttcgcc accaccacgg
      181 aagatcgcag cctggtggac atgatcttca acgggctggt ccgctataag ccgggcgatg
      241 gctcggtctt cgagccagac ctggccacga gcttgccgac gccgaggatg gagggcggca
      301 agcaggtgtg ggcctttcag cttcgcaagg gggtcatgtg tcacccgatg agcggcgtgc
      361 cctcctacga gctgaccagc gaggacgtcc tgtattctct gcagaagtcg gcggacaaga
      421 atcggtcagc ctacgccgcg gattacacgg ggatgacctt cgaggcgccg gacgcctcca
      481 cgctgaagat ctcgctggag aagccgctat cgccgatcct gttttttgcc aaggtggcca
      541 actattcggg cggattcatc gtctgcaaga aggccgcgga gaagctgggc cccgacgcct
      601 tgaaaaccca ccccgtcggg accggcccct tcatgttcaa gagctacagc ccgcaggagc
      661 gggtgcaact ggtggcgaat cccgcctact tccgggggaa gccgcaactg gatggggtgg
      721 actatcgctt catgccggat ctgagcagcc gggagctggc cctacgtaat ggtcagatgg
      781 atgtgatcaa cggggccccc gacaaggcct gggtggacaa gatgaagggg gtctccggcg
      841 tcaaggtgga cgtcttcggt gtgggcgagg tggcgtcgct ttacttcaac acgaacgccc
      901 agccgctcaa caaggcggag gcgcgcaagg ccatcgccta cgccctggac cgggacgagt
      961 tcctggctct ggtgggcccc ggaatcgcag agaacgtcta ctcccccgtg cccgtcaagt
     1021 tcctggccgg cggccttacc cggcaggagc tggcggccaa gggcctggac tacaagaacg
     1081 atctggcgaa ggccaagaag ctcctggcgg acgccggtta tcccaacggc ttttccctgg
     1141 aggtgctcac ctccgagatg accgggtaca aggtgctcta cgaatccatg caggcccagc
     1201 tcgccaaggc gggcatcaag ctcaacgtga agctggtgga ccattcgagc ttccacagca
     1261 tgatccgcaa ggacgccaac ccgctggtaa tctacatcgc ctggcgtccc aatgcggacg
     1321 tgttcctcac gcagttcctg cactcagatt cgatcgtcgt caccggcaag aagcccaaca
     1381 ccaacttctc gcactatgac aagatcgacg ggctcatcga gcaggcccgg aacgagattg
     1441 atccggcaaa gcagatagcc ctctggaaag aagcccagat caaggccctg gaagacatgg
     1501 tcgtgtatcc catccagtac cagaaccagg tctacgcccg gtcgaccgcc gtggacttcg
     1561 ggcacgatct catctcggtc ctggcgctct atcccggcat cgacgagaag acccgcctga
     1621 cgaagtaacc gaactccaac cgcgcggtgg gcgcgccaac aggcgggcgc gtccaccgcg
     1681 tttggtctct cgccaccgag cggccgtgtt caaatacatc gcgcaacgac tcgtcctttc
     1741 gatcccagtg ttcctgggcg tggccaccat tgtcttcctg gtggtccgcg tcattcccgg
     1801 cgaccccgcc gttgccgcct tgggtgacta tgcgtccaag gaggcggtgg aggccctgcg
     1861 ggaacggatg ggcctgaatg catcgctgcc ggttcagtat gtcaggttcc tgggcggcct
     1921 cgtccacggg gatctgggca agtccatgat caccggcacg tcgatccgtg ccgatatcct
     1981 gcacgccctt ccctatacca tcgagttgac tgtcctggcg atcatcatcg gcactctcct
     2041 ggggatcccg atcggcgtct acactgccat ccgtcgcaat cgaatggcgg attatcagtg
     2101 gcgggtccag
//
LOCUS       DBNGZC010001117         1365 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2051601, whole genome shotgun sequence.
ACCESSION   DBNGZC010001117 DBNGZC010000000
VERSION     DBNGZC010001117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1365)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2051601"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            429..632
                     /locus_tag="AC16G0_19310"
                     /pseudo
     CDS             429..632
                     /locus_tag="AC16G0_19310"
                     /inference="COORDINATES: protein motif:HMM:NF024412.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            613..>1365
                     /locus_tag="AC16G0_19315"
     CDS             613..>1365
                     /locus_tag="AC16G0_19315"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HIR5101811.1"
                     /translation="MSGDGPNASDVTAWPASATGLDPAARRSFPPEVALHLVKMACER
                     PEDRGRSLALWDCREMARQLVSEGVVPSISPETVRRVLEHHHLKPWRQHLWLSPEVPR
                     DAAFAAAVREISDLYTRPLKADEMVQCVDEKTSLQPRPRKSPTLAAQPGRSVRVEHEY
                     GRCGALNLFAAFDTRTGKVYGRTAERKRQVEFIAFLEQLDRGIPATITTIPVILDNLW
                     MHKGQQVQAWLAQPPRFIFHHPPVHCSWMNQVE"
BASE COUNT          242 a          454 c          454 g          215 t
ORIGIN      
        1 cgacaaagcc actccaggga agggtgtaga gcggaaagcg cgtccgcgtg cggccccaga
       61 ggaaccggtc gcctgtctcg gaacttcgtt cgtcgtgccg agctgtgcct gaggaatcgc
      121 accacaaaga cacaaagaag gcgcaaaaac acgaaggtta acgcgagggc accatgaatg
      181 ctccgatcgg cgcaccttcg atcattatgt tcaggaaacc acggcctttg gccgtgcgac
      241 ccgaagagct tcgagcgatc cggcgtgcct tctgccgagc ccctcggctc tgtccggggt
      301 gacggcgagg gcctaagtac aacgccgcag agatagggta ccgaattgac gaaacggcat
      361 gggcatggcg attcttgggg cttgaggggg actttccttt ctacgccctt tgaaccttcg
      421 gtgtcgccat gcgcggccgc aaatccgccc tgaccatcgt cctgaccccg gccgaccgag
      481 cggaaatcga gcgctggcaa cgctccacca aaggccccgc cggcttggct cgccgcggcc
      541 gcatcctcct gctgctcgaa ggcggcctga ccctgaagga caccgccgag cggtgcggga
      601 tgagcgtgcg gcatgtccgg agatgggccg aacgcttccg acgtcacggc ctggccggcc
      661 tccgcgaccg gcctcgaccc ggccgcacgc cggtcttttc cccccgaggt ggcattgcac
      721 ctggtcaaga tggcctgcga gcgacccgag gaccgcggcc ggtcgctggc gctgtgggac
      781 tgtcgcgaga tggcccgtca gttggtctcc gagggggtcg tgcccagcat ctccccggag
      841 accgtccggc gggtcctgga gcaccatcac ctcaagccct ggcggcagca cctctggctc
      901 tccccggagg tcccacggga cgcggccttc gcggcggcgg tccgggagat cagcgacctg
      961 tacacccggc cgctcaaagc ggacgagatg gtccagtgcg tggatgagaa gaccagcttg
     1021 cagccccgcc cacgtaagtc gccgacgctc gcggcccaac ccgggcggtc ggtccgcgtc
     1081 gagcacgagt acgggcggtg cggggccttg aacctgttcg ccgccttcga cacccgcacg
     1141 ggcaaggtct acggacggac ggccgagcgg aagcgacagg tggagttcat cgcgttcctg
     1201 gagcaactgg accgcgggat cccggcaacg atcacgacga ttcctgtgat cctggacaac
     1261 ctgtggatgc acaagggcca gcaggtgcag gcgtggctgg cgcagcctcc gcggttcatc
     1321 ttccatcacc cgccggtgca ttgctcgtgg atgaatcagg tggag
//
LOCUS       DBNGZC010001118         2137 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2051764, whole genome shotgun sequence.
ACCESSION   DBNGZC010001118 DBNGZC010000000
VERSION     DBNGZC010001118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2051764"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2137)
                     /locus_tag="AC16G0_19320"
     CDS             complement(<1..>2137)
                     /locus_tag="AC16G0_19320"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HIR5101812.1"
                     /translation="TDQDEPFASFYEQRRILVPLAKIPIQLKQAVLSVEDSHFYEHHG
                     LHPRAILRAMIMNVLSRRKAQGGSTITQQLARVLFLTPEKSFARKVKEALLALEIEKR
                     YSKDRILELYFNQVYFGHGAYGVEAAAQTYFKKSVGDLTLPECALLAGLPSAPNRFSP
                     IVDPARARRRRDHVLSRMVDEKYITRAQAEVASRTAFDETVFTRSRTIAPYFVEHIRQ
                     ALEETYGAYALYNSGLKVYTTLNLKMQRGAEEALVGGLREIDKSHGYRREPPKAAEIA
                     RTRIGLYTPRVGEILPGTVVMVKAKSLGVQLGRYRGEIPFDSLKWTKLDNPSLAFRGG
                     APILVRVLAVDERRKVVELALDQDPELEGAFVALDPRDGGIRAMIGGYDFERSKFNRA
                     TQAKRQPGSAFKPFVYAAAFDRGFTPSTIIDDSPISFHFHAGGKAVEWSPENYDRKFH
                     GPTTLRRGLENSVNVVTVKLMERVGVDQVIRMAHQVGIESDLRREVALALGVSEVTPL
                     ELVSAYGVFANGGVRVEPFAIRKVTDSQGRTLEEHVVEPQEVMRPETAYVLVHVMKGV
                     VERGTAARARVLRRPLAGKTGTSSEAPDVWFVGFTPSLVAGVWIGYDVKRSLGSAETG
                     GRMALPLWINFMQKVLADTPPEDFVVPDNVVGMPVNPFTGRPVSPDERGSIMEYYIRG
                     TEPKISEVTAASPGSPPSPSPSPASPSPPP"
BASE COUNT          395 a          672 c          677 g          393 t
ORIGIN      
        1 ggggggaggg gagggagagg cgggagacgg cgaaggcgag ggaggactgc ccggcgatgc
       61 agcggtgacc tctgatattt ttggctcggt tccccgaata tagtattcca tgatgcttcc
      121 ccgctcatcg ggggaaacgg ggcggcccgt gaatggattc acgggcatgc cgaccacgtt
      181 gtccggtacc acgaagtcct cgggcggggt atccgccaag accttctgca tgaaattgat
      241 ccagaggggc agcgccatcc ggccgccagt ctccgccgag cccaatgagc gcttgacgtc
      301 gtagccgatc cacactcctg cgaccaggct tggggtgaaa ccgacaaacc atacatctgg
      361 ggcttcggag ctggtgcccg tcttcccggc caggggtcgg cgcaacacgc gagctcgcgc
      421 cgccgtcccc cgctccacga cccccttcat cacgtgcacc agaacgtagg ccgtctccgg
      481 acgcatgacc tcctgaggct cgaccacgtg ttcttccagg gtgcgcccct gactatcagt
      541 taccttgcga atggcgaagg gctccacccg gacaccgcca ttggcgaaca caccataggc
      601 cgacaccaac tctaagggcg tgacttcgga gacacccaag gccagagcca cctcgcgccg
      661 gagatcgctc tcgatgccca cctggtgggc catgcggatc acctggtcca cccccacccg
      721 ctccatcagt ttcacggtca cgacgttgac ggagttctcc aggccccggc gaagggtcgt
      781 cggcccgtgg aatttccggt cgtagttctc cggagaccat tcaaccgcct tcccaccggc
      841 atggaagtga aagctgatgg gggagtcatc gatgatggtg gacggagtga agccgcgatc
      901 gaaagcggca gcatacacga aaggcttgaa ggcggatccc ggctgacgtt tcgcctgcgt
      961 ggcccggttg aacttgctcc gttcgaaatc gtacccgccg atcatcgccc ggatcccccc
     1021 atcccgagga tcgagggcca cgaaggcccc ttcgagttcc gggtcctgat ccaaggccag
     1081 ctccacgacc ttccgccgct catctactgc gagaacgcgc accaggatcg gtgccccccc
     1141 gcgaaaggcc agggaagggt tatcgagctt ggtccacttc agactgtcga aggggatctc
     1201 gccccggtat cgaccgagct ggacccccaa ggacttggcc ttcaccatga cgacggtccc
     1261 cggcagaatc tcgccaacgc gtggcgtgta gaggccaatg cgggtccggg cgatctcggc
     1321 agccttgggc ggctctcgac ggtagccgtg actcttgtcg atctcacgca gaccgcccac
     1381 caaggcctcc tcggccccac gctgcatctt caagttgagg gtggtataca ccttcaggcc
     1441 gctgttgtag agggcgtaag ccccgtacgt ttcctccagg gcctggcgaa tgtgctccac
     1501 gaagtaggga gcaatggttc ggctccgggt gaagaccgtc tcgtcgaagg cggtccggga
     1561 ggccacctcg gcctgggccc gggtaatgta tttctcatcg accatgcggc tcaggacgtg
     1621 gtcgcgtcgg cggcgggcgc gagcgggatc gacgatgggg gagaatcggt tgggcgcact
     1681 cggaagccct gcgagcagcg cgcactccgg gagggtgaga tctcccaccg acttcttgaa
     1741 gtaggtctgg gctgccgcct cgaccccgta ggctccatgg ccgaagtaga cctggttgaa
     1801 gtacagttcc aggattctat ccttggaata ccgcttctcg atctccagcg ccaggagggc
     1861 ttccttcact ttccgggcga agctcttctc aggtgtcaag aagaggacgc gggcgagttg
     1921 ctgcgtgatg gtgctgcctc cttgggcctt ccgccgcgag agaacattca tgatcatggc
     1981 ccggaggata gcccgaggat gcaggccgtg atgttcgtag aagtgtgagt cctccacgga
     2041 caacacggcc tgcttgagct ggatggggat ctttgcgagc gggaccagaa ttcgtcgctg
     2101 ctcgtaaaac gaggcgaagg gttcgtcctg gtccgtg
//
LOCUS       DBNGZC010001119         1913 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1661478, whole genome shotgun sequence.
ACCESSION   DBNGZC010001119 DBNGZC010000000
VERSION     DBNGZC010001119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1913)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1913)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1913
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1661478"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="AC16G0_19325"
     CDS             <1..428
                     /locus_tag="AC16G0_19325"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR5101813.1"
                     /translation="TGARHHGYCSDITRTFFLGPPPAAYVEIYTLELTVSRRVLEEIR
                     AGVSLSHLDETAHRIVDEAGYGQYFPHRVGHGLGIEGHEAPSVVTGNATVTTPGLTFT
                     VEPGIYIPNLGGVRVEENVAVTAEGADVLTAYPRELRSL"
     gene            complement(591..980)
                     /locus_tag="AC16G0_19330"
     CDS             complement(591..980)
                     /locus_tag="AC16G0_19330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5101814.1"
                     /translation="MGESWREPWVLVVEDTELTRCLIADELREAGCQVWTAADGVEAV
                     EILETRRVDLLFTDCDMPRMNGLELIRWSRAHLPHIPTVLMTGHDRDTIGADAWDCGT
                     LRILLKPFSIQHVLLLVKELRGTAVEG"
     gene            complement(1446..>1913)
                     /locus_tag="AC16G0_19335"
     CDS             complement(1446..>1913)
                     /locus_tag="AC16G0_19335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101815.1"
                     /translation="RHAGNKAIEWPIAALLYPVAGLLCGQLWRFYPEERTLFVFSIAL
                     PTMSLFLSIWVGGVTTQAALQHLIPCFPRAVDLRRKGRAEWFLGTFRCHRYWLLATRA
                     SWRVLGSLLLTGHYEARKTDRLIRPVMTPSPGAVRRPILNSPERGIQTGFPGD"
BASE COUNT          361 a          588 c          637 g          327 t
ORIGIN      
        1 acacgggggc tcgccaccac ggctactgca gcgacatcac caggaccttc tttctgggcc
       61 cgccacccgc ggcctacgtg gaaatctaca cgctggagct cacggtcagc cggcgcgtcc
      121 tggaggaaat ccgggccgga gtcagtcttt cgcacctgga cgaaacggcc catcggatcg
      181 tggacgaggc gggctacggt caatacttcc cgcatcgtgt gggccacggt cttgggatcg
      241 agggacacga ggccccttcg gtggtcacgg gcaatgccac agtcaccacg ccgggtctca
      301 cctttactgt cgagcccggg atctacatcc cgaacctggg tggcgtgcgt gtggaggaga
      361 acgttgccgt tacggcggag ggtgcggacg tgctgactgc ctacccgaga gagcttcgct
      421 ccctttgacc gggcacggac gccgcggcag gtgcgggcac caggccagat cggcaggcct
      481 cttttaggag agacacgcgg tggcgtcgcg taagaattcc gcaggcgggc aggatgctcc
      541 tggggggacg agtcgggcga cggggccagg aagctatgag gggaggccga ctatccttcg
      601 accgccgtgc cccgcagctc cttcacgagc aggagaacgt gctgtatgga aaagggcttt
      661 aggaggatgc gcagcgttcc gcaatcccag gcgtccgccc cgatcgtatc gcgatcatgt
      721 ccggtcatca acaccgtggg gatgtgaggc aggtgtgccc ggctccagcg gatgagctcc
      781 aggccattca ttcggggcat gtcgcaatcg gtgaagagga ggtccacccg ccgcgtctcg
      841 aggatctcca cggcctcgac cccgtcggcg gccgtccaca cctggcaacc cgcctccctc
      901 agctcgtcgg caatcaggca gcgtgtcagc tcggtgtcct ctaccacaag cacccagggt
      961 tcgcgccagc tttcccccat caggtcctct cctctcaacc cgaaggctgg acttattctt
     1021 ccggaagaaa tccttcctga tccttcgttc gaatcagctt ctccgatgag atcgggcttc
     1081 catcacgtta gcaaccgtct cagggatctt ccgggggtag gctccgcaga atcgagccgt
     1141 actggggcgg ggtcagcccc ggtctgtgga tatggatacc actcgagcgg cgagggggga
     1201 atcagcagaa ggctgattct tgtgtcagaa agggacacac gtcggcgtcc gtgcgggtcc
     1261 tacggggatc aggtggagaa gggacatggc gggcactctg ggggccatat ctggactggg
     1321 agaggaagga agaggaggcg tgccgtccag atgcacaacg acccactggg ggcggtggac
     1381 ctccagaggg tctgcaagct cgtatcccgg agcctatccg gccggcatcg ccaccctctc
     1441 cgggctcagt cgccggggaa ccccgtttgg atgccccttt ccggggagtt gagaatgggg
     1501 cgtctcacgg cgccggggga tggcgtcatg actgggcgga tcaggcggtc agtctttctt
     1561 gcctcgtagt gtccggtgag gagcaggctc ccaagcacgc gccacgaagc ccgggtggcc
     1621 aatagccagt accgatggca ccggaaagta ccgaggaacc actccgcccg ccccttgcga
     1681 cggaggtcca ctgcacgcgg aaaacacgga atgaggtgct gaagtgcagc ctgggtggta
     1741 accccaccga cccagatgct caggaagagc gacatcgtcg gcagggctat tgaaaacaca
     1801 aacagggtcc tctcctccgg gtagaaacgc cagagttgtc cgcagaggag accggccacc
     1861 gggtagagca gggcggcaat gggccactcg atggccttgt tgcccgcatg gcg
//
LOCUS       DBNGZC010001120         2376 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1760538, whole genome shotgun sequence.
ACCESSION   DBNGZC010001120 DBNGZC010000000
VERSION     DBNGZC010001120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1760538"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..520)
                     /locus_tag="AC16G0_19340"
     CDS             complement(32..520)
                     /locus_tag="AC16G0_19340"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR5101816.1"
                     /translation="MSFLPRFSRCLEILTAGLLVVVVGLVSAQVVARYVFNASLTWSE
                     EAARMTFVYLTFLGAALALGRNQNLKIDLFVGRLSRRARLWLDLALHSLSLVFVLVVL
                     VYSVPLLQRLYPTPTPALQWSMVTFYAGIPAGSLAMLVYLVRNVRAGVTELRRGEAGP
                     PC"
     gene            complement(567..1862)
                     /locus_tag="AC16G0_19345"
     CDS             complement(567..1862)
                     /locus_tag="AC16G0_19345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR5101817.1"
                     /translation="MIDVSIIVFGVFLVLVVLGIRIAFALGATACLIILLYHQGAIGI
                     SSIFYSNMAKFPLLAIPYFILAGFVMERSGVSARLVNLASLIVGPIPGGLAIVGVAVC
                     VFFGGISGSGPADAAAIGAVLIPAMIEKGYDRNFAAALIATAGSTAILIPPSIAFVVY
                     GVITEVSVPALFAGGIFPGLVAGAALIVPAYLISKKRGWGGERWGTPRELWIAFRRAF
                     WGLMAPAVILGSMYGGICTPTEAAIIAVVYGVVLGLLYRTIGWAELYACMRDAAVTSA
                     VVMFIVAFAGLFGWAADRVGAIDHVLKSLLSLTNNGLVMIFLVNGVLFIAGMLLDAIS
                     IFYVFLPIFIPMMSHFGWDPLWFGVVMTYNLAIGQATPPVAVNLYVTTALAKSKLDQV
                     AVAVVPFVVAMVGGLIVVILWQWLSLAPPIWFGLYKLGQ"
     gene            complement(1859..>2376)
                     /locus_tag="AC16G0_19350"
     CDS             complement(1859..>2376)
                     /locus_tag="AC16G0_19350"
                     /inference="COORDINATES: protein motif:HMM:NF016200.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR5101818.1"
                     /translation="RVFLTPHGGAMSEGLRWLGRNAEEVVCAAMMIFMTVLGFANVAA
                     RYLGYSMAYTEELLVMLLVWLTMLGTAAGFKRNAHLSMTFLRERLPNAGQKTLEALSM
                     GLTVGAVLVVMIVCAVYQIPDEILLHSRTLSLDLPTVYYSLAIPVGGVLVIVRVLQAS
                     WRDWKSLGRAG"
BASE COUNT          519 a          793 c          761 g          303 t
ORIGIN      
        1 atcaagacca gcatgaccac gaagagcaag gtcagcatgg cggtccggcc tcgccccgtc
       61 gcagctccgt cacccctgcc cggacgttcc gcacgaggta gacgagcatg gccaggctgc
      121 cagccgggat cccggcatag aacgtcacca tggaccactg gagggccggc gtcggcgtcg
      181 gatacagccg ctggagcaac gggacgctgt agacgagcac cacgaggacg aagaccaggg
      241 acaggctatg caatgcgaga tcgagccaca accgggcccg ccggctcagc cttcccacga
      301 agagatcgat cttcaggttt tgattgcgac ccagcgccag ggccgcccca aggaaggtca
      361 gatacacaaa ggtcatccgg gcggcctcct cgctccaggt cagggaggcg ttgaagacgt
      421 agcgcgccac gacctgggcc gagaccagac cgaccacgac gacaagcagg cccgccgtca
      481 ggatctcgag acaccggctg aagcggggga gaaagctcat ggccgcgacc acgcgagcga
      541 catcacccca aaacggttga aggccttcac tgacctagct tgtacagccc gaaccaaatt
      601 ggcggtgcca gggacaacca ctgccagagg atgacgacga tcaatccacc caccatggcc
      661 acgacgaacg gcacgaccgc cacggccact tggtcgagct tgctcttggc cagggcggtg
      721 gtcacgtaca ggttcaccgc caccggcggc gtcgcctggc cgatggccag gttgtacgtc
      781 atcaccaccc cgaaccagag cgggtcccac ccgaagtggc tcatcatggg gatgaagatc
      841 ggcaggaaga cgtagaagat ggagatggca tccaggagca tcccggcgat gaagagcacc
      901 ccgttgacga ggaagatcat caccaggccg ttgttggtca ggctgagcag gctcttgagc
      961 acgtggtcga tggcgcccac gcgatcggct gcccagccga agaggcccgc aaaggccacg
     1021 atgaacatga ccaccgccga ggtgaccgcc gcatcccgca tgcacgcgta cagctccgcc
     1081 cagccgatgg tccggtagag caaccccagc accaccccat acaccacggc gatgatggcg
     1141 gcctcggtcg gggtgcagat accgccgtac atgctcccca ggatcaccgc cggtgccatg
     1201 agcccccaga aggcccgcct gaaggcgatc cagagctccc ggggggtccc ccaccgctcg
     1261 cccccccagc cccgcttctt cgagatgaga taggccggca cgatgagcgc cgccccggcc
     1321 accagcccgg ggaagatgcc cccggcgaac agggccggga ccgagacctc ggtgatcacg
     1381 ccgtagacaa cgaaggcgat ggagggcggg atcaggatgg ccgtggagcc tgcggtggcg
     1441 atgagggcgg ccgcgaagtt gcggtcgtac cctttctcga tcatggccgg gatcagcacg
     1501 gcgccgatgg ccgcggcgtc cgccggaccg gagccggaga tcccgccaaa gaagacgcag
     1561 accgcgaccc ccacgatggc cagtccgccg gggatgggcc cgacgatcag gctcgccagg
     1621 ttgaccagtc gtgcggagac ccccgaccgc tccatgacga acccggcgag gatgaagtac
     1681 gggatggcga gcagcgggaa cttcgccatg ttggagtaaa aaatggacga gatgccgatc
     1741 gcgccctgat gatacaagag aatgatcagg caggccgtcg cacccagcgc gaacgcgatg
     1801 cggatcccca gcacgacgag gaccaagaac accccgaaga caatgatgga aacgtcaatc
     1861 acccggccct cccgagactc ttccaatccc gccaggaggc ctggaggacc cggacgatga
     1921 ccaggacgcc gcccacgggg atggcgaggc tgtagtagac cgtgggcaaa tccagggaca
     1981 gcgtccgact gtgcaggagg atctcatcgg ggatctggta gaccgcacag acgatcatca
     2041 ccacgagcac cgcaccgacc gtcaggccca tcgagagggc ctccagggtc ttctggccgg
     2101 cgttggggag tcgctctcga aggaaggtca tgctgaggtg ggcgtttcgc ttgaacccgg
     2161 ccgcggtgcc cagcatggtc agccagacca gcagcatgac cagcaactcc tccgtgtagg
     2221 ccatggagta ccccaggtac cgcgccgcca cgttcgcgaa tcccagcacg gtcatgaaga
     2281 tcatcatggc cgcgcacacg acctcttccg cgttgcggcc gagccagcgc agtccctcgc
     2341 tcatcgcgcc cccatggggt gtgagaaaga cgcggg
//
LOCUS       DBNGZC010001121         9290 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1760913, whole genome shotgun sequence.
ACCESSION   DBNGZC010001121 DBNGZC010000000
VERSION     DBNGZC010001121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 9290)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9290)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9290
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1760913"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..1122
                     /locus_tag="AC16G0_19355"
     CDS             25..1122
                     /locus_tag="AC16G0_19355"
                     /EC_number="1.17.98.2"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5101819.1"
                     /translation="MSRRIAYRLKRRTRGRPFESIQIEVTSRCGMRCRMCPKVALATH
                     WTELDLSWETFHAIAQAFPLVRHVHLQGWGEPLLHPRLFEMIALAKGAGCRVGLTTNG
                     MDLDQEAGRRLLELQLDVLSVSIAGATRETHEGIRGRTDFCLILENVRHLLEARRGRG
                     GKGPKVELSYLMTRTNISELPRAVELAAALGVDELYATNLDYLAASAHQNLAVFACPS
                     HRESFARSLEEARNTARRLGLSFRPYPLDLEEVAVCEAHPTKTLFISSEGWVAPCTYL
                     TLPGQTQIPRWFGGGQVAVPAMRFGNVRDQDLLDIWEAPAYREFRRRFADRRLGLATR
                     TLAAMSAGGSADSEMPSPPEPCRSCYKLLGV"
     gene            1336..2874
                     /locus_tag="AC16G0_19360"
     CDS             1336..2874
                     /locus_tag="AC16G0_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007527360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HIR5101820.1"
                     /translation="MPATKEKGSGGSTLIKSEDWMTVWLGFLIILLVLVGLRFKTPGF
                     RWMSDGDFQSVVAQNTPAVDNLVKVAQEKNEAGLAAAATALKAAMGAQDRQAIGDAAK
                     KMGAAAAQDAGLKKTAAGISRKLSGDAGATMGKVFSGDNLWTSILIGVGFLILAAIGN
                     ALMGRSVGKFIIGFPVVFILTWLSLFIAGNTTVTYYGLEFALWALILGLFISNVLGLP
                     TWMKEAVRTEYYIKVGLVVMGAGILFGEILQAGAYGVIQSVVVVVAVWYFAYWLGQKL
                     RVDDEFSAMLATAVSICGVSAAIAAAGAIQGDKKKLSYTTSIVLVVAVPMMILMPIVG
                     KAIGLPELVAGAWIGGTIDTTGAVVAAGALYGDAAMKVATIVKFSQNVLIGFAAFLLA
                     IAWSVKRGAETGEKPSAMVIWERFPKFVLGFLVASLVFSFFLSPDTINAVKGPLNFIR
                     LLTFALAFVCIGLETNFKELLQLEGGRPAAAFLGAQAFNLIWTLIIAFLLFGGILFSM
                     PVIK"
     gene            2986..3219
                     /locus_tag="AC16G0_19365"
     CDS             2986..3219
                     /locus_tag="AC16G0_19365"
                     /inference="COORDINATES: protein motif:HMM:NF014950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5101821.1"
                     /translation="MASDLSLRPGRLEPSLPRDQERSLGKVREDAERDHILSVLKLTE
                     GRRGRAAQILGISRKTLWKKLKHLGVSRSLDVT"
     gene            3385..4491
                     /locus_tag="AC16G0_19370"
     CDS             3385..4491
                     /locus_tag="AC16G0_19370"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5101822.1"
                     /translation="MPIVVKKVRRLGAGGGGAGGGGSTSSLRPEFVPKTPACSKACPN
                     HHDIRAVLTTIAQADAHGKTSDQALTEAFCLLTDLNPLPASCGRACPHQCELQCYRLD
                     VDEAVSINCLERYIGDYALEKGLPLTPLTADRHPERVAVIGSGPAGLSCAYQLARRGY
                     SVTVFEAFPRPGGMLRYGLPSYRLPRQVLDGEITRLGSLGIEIRCNTALGKEVTLDAL
                     RQEYSAIFVGIRAQLGTKLGLPGEDAPNVLLAAEFLHRVNGGERLVVGGRVLVVGGSD
                     TAVDAARVARRLGAQATIVSRRPTQEMPAIRHEVEEAQREGVQIEGPAVPLVIVTEAG
                     RATGLTCRRETPSGHPGSAESEFFLPADSIIVAA"
     gene            4729..5037
                     /locus_tag="AC16G0_19375"
     CDS             4729..5037
                     /locus_tag="AC16G0_19375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101823.1"
                     /translation="MRLGYFTKQPRTPLATIPLEERLQHPEQEVNLGLTETQIIAEAR
                     RCLSCGLCFECDTCWQYCQEQAIQKPAEKGSPYRIKLEFCTGCKKCAEQCPCGYIEMR
                     "
     gene            5080..6294
                     /gene="dsrA"
                     /locus_tag="AC16G0_19380"
     CDS             5080..6294
                     /gene="dsrA"
                     /locus_tag="AC16G0_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - dissimilatory sulfite reductase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     alpha"
                     /protein_id="HIR5101824.1"
                     /translation="MAADEKKLPPTPKLDELESGPWPSFVKEIKRAAEKNPMSQDLLG
                     VLELSYTQKIAHWKHGGIVGVRGYGGGVIGRYCDIPEQFPNVAHFHTLRVNHPAGWFY
                     TTKALRNICDLWERHGSGLTNLHGSTGDIILLGTRTEELEATFGELTQAGFDLGGSGS
                     VIRTPSCCVGPARCEWACFDTLRFIYDVTMHFQDSIHRPSFPYKFKIKASGCANDCVA
                     AIARADLSIIGTWRGPIQVDQDAVGAYAKDGIDIQKDVCDPCPSKCISWNGTTLAIDN
                     PWCVKCMHCINVMPKALRPGRERGATILIGGKAPILKGAMLSSVLVPFMQMDPPYTEF
                     TALAEKILDVWGEEGKNRERVGEFIQRVGLGNFLEAIGLEPMPEMVAHPRENPYIFYE
                     EYLEEESTQSQS"
     gene            6342..7424
                     /gene="dsrB"
                     /locus_tag="AC16G0_19385"
     CDS             6342..7424
                     /gene="dsrB"
                     /locus_tag="AC16G0_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0018551 - dissimilatory sulfite reductase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     beta"
                     /protein_id="HIR5101825.1"
                     /translation="MAETAVQRKTDIGPPHYQMFLPPMVKKNYGQWRYHDILKPGVLV
                     HVAESGDKLYTVRAGTPRLLSIQTIREICDLADTYCDGYLRFTSRHNIEFLLTDSAKI
                     EPLITDLAARGFPVGGAGNAISSMVHTQGWIHCHTAATDASGIVKAVMDELIDHFTTT
                     KLPAKLRLALACCLNMCGAVHCSDIAILGIHRRPPKVDHQRLSKVCEIPNVVASCPTA
                     AIRPTRIDGKETVEIIEENCMFCANCYTVCPALPINNARNDGCSIWVGGKVSNARHAP
                     MFSKLAVPFLPNNPPRWPEVVAAVKNIVEVWAADARKYERMGEWIERIGWPRFFQKTG
                     IPFTKQHIDDYRHADQTWQRSIHFKA"
     gene            7435..7692
                     /locus_tag="AC16G0_19390"
     CDS             7435..7692
                     /locus_tag="AC16G0_19390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101826.1"
                     /translation="MAEPVSTEELAEAMYHMVQESAGSRRLRPTDLTRAMIERFGPER
                     CTKEHCKQALRRLVDAERCVYTFYDGSYVELPPQEKGKAEA"
     gene            7713..9206
                     /locus_tag="AC16G0_19395"
     CDS             7713..9206
                     /locus_tag="AC16G0_19395"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinic acid a,c-diamide synthase CobB"
                     /protein_id="HIR5101827.1"
                     /translation="MLDPVSVHTAACPRVMLCGLRGSTGKTLVALGLISAWEARGVSV
                     APCKKGPDYIDPAWHTRAGTRPCRNLDTFLMSDEQIRRSVGEHGRTADLALIEANHGI
                     FDGVGAEAAASSAELAKLLQAPVVLVVDCTKTTRTAAAMVLGCQHMDPDLRLAGVILN
                     RVATERQERAVREAVETTCGLPVLGSLPRLSNLEFPERHLGLLTPAEHPAVAEPIRIA
                     RQAVEAHVDLDAIQRLAATAPPLAWTAEAESRIDGRGVRIGVVRDSAFTFYYPENLEG
                     LERQGAEIVEVNALKDPDLPPLDALYIGGGYPETHAAALAANVGFRGALHREIEAGLP
                     IIAECGGLIYLGEAMVTDGFAHPMVGIFPVTFELGKRPWGHGYTVVEVDTPNPFFAPG
                     TALRGHEFRYSYVRSARTEDLRLAFRMQRGTGFDTKRDGLVHKNVLASFCHFHAAGTP
                     EWAPALVRQAHLHAQRRRLAARSRDVLHRDWTTPEPAGSVALPVGTI"
BASE COUNT         1668 a         2974 c         2998 g         1650 t
ORIGIN      
        1 caccagcctg tgagctagcc atcaatgtcc agacgcatcg cctacaggct caaacgacgg
       61 acccgagggc gaccctttga gtccatccag atcgaggtga cctcccgttg cggcatgcgc
      121 tgccggatgt gccccaaggt cgccctggcg acacactgga cggaattgga tctgtcctgg
      181 gagaccttcc acgccatcgc gcaggccttc ccgctggtcc ggcacgttca cctgcagggc
      241 tggggcgagc ccctcttgca cccccgcctc ttcgagatga tcgccctggc caagggggcg
      301 ggctgccggg tgggactgac cacaaatggc atggatctgg atcaggaggc gggaaggcgt
      361 ttgctggagc tgcaactgga tgtcctgtcc gtctccattg cgggcgcgac gcgggagacg
      421 catgagggca tccgggggcg caccgacttt tgcctgatcc tcgagaacgt ccgacacctc
      481 ctggaggcgc gtcgtgggcg aggggggaag ggaccaaagg tcgagctttc ctatctcatg
      541 acccggacca atatatcgga gttgccgcgg gcagtggagc tggccgcagc cctcggcgtg
      601 gatgagctgt acgccaccaa ccttgactac ctcgcggcat cggcgcacca gaacctcgcc
      661 gtcttcgcct gtccttcgca tcgggagtct tttgcccgca gcctggaaga ggcccgaaac
      721 acggcccggc gccttggcct gtccttccgt ccctacccgc tcgatctcga agaggtggcc
      781 gtctgcgagg cacacccgac gaagaccctg ttcatctcca gcgagggctg ggtcgctccg
      841 tgcacgtatc tcacgctacc cggtcagacc cagatcccac gatggttcgg aggcgggcag
      901 gtagcggtgc cagccatgcg atttggaaac gtccgggatc aagaccttct cgacatctgg
      961 gaggctccgg cctaccggga gttccgccgg cggttcgccg accggcggct ggggctcgcc
     1021 actcggaccc tcgctgccat gtcggctggc ggtagcgccg attcagaaat gccctctccg
     1081 cccgagccgt gccgcagttg ctataagctg ctcggggtat aacccctcag tctttccgtt
     1141 acccctcggt aacactcgcc ggtcctgctg ttgccttttg gtaacccccg gggtcaaccc
     1201 caccgccatc ccaacaccca acttcccgat tcttcgcgtg ttgcacgccc acaagctggc
     1261 ggcagcgttc ttgctatctc ccacaggtgg gacaggggtg ctcagatcgt tcccatcaga
     1321 gaggaggaag cgacaatgcc tgcaaccaag gagaaaggga gtggaggctc taccctgatc
     1381 aagagcgagg actggatgac ggtctggctc gggttcctca tcatcctgct tgtcctggtc
     1441 ggtctccgat tcaagacccc aggcttcagg tggatgagtg acggggattt ccaatccgtg
     1501 gtggcgcaaa acacgcctgc ggtggacaac ctggtcaagg tcgcccagga aaagaacgaa
     1561 gcgggtcttg ccgccgccgc cacggccctc aaggccgcca tgggggccca ggaccgccag
     1621 gccatcggcg atgccgccaa gaagatgggc gcggcggcgg cgcaggacgc cggcttgaag
     1681 aagactgcgg ccggaatcag tcggaagctc agcggggatg cgggcgccac catggggaag
     1741 gtcttctcgg gggataacct ctggacgtcc atcctcatcg gtgtgggctt cctgatcctg
     1801 gccgccatag gcaatgcgct catgggcagg agcgtgggaa agttcatcat cggtttcccg
     1861 gtcgtattca tcctgacgtg gctgtccctg ttcattgcgg gcaacaccac cgtcacgtat
     1921 tacggcctgg aatttgcgct gtgggcgctc atcctcgggc tgttcatcag caacgtcctc
     1981 ggccttccaa cctggatgaa ggaggcggtc cggacggagt actacatcaa ggttggattg
     2041 gtggtcatgg gggccggaat cctctttggg gagatcctcc aggcgggcgc ctacggggtg
     2101 atccagtccg tcgtggtcgt ggtggccgtc tggtatttcg cctactggct gggccagaag
     2161 cttcgggtgg atgacgagtt ttccgccatg ctggcgaccg cggtctcgat ctgcggcgtc
     2221 tcggcggcca tcgccgccgc gggggcgatc cagggcgaca agaagaagct cagctacacc
     2281 acttccatcg tcctggtggt cgccgtgccg atgatgatcc tgatgccgat cgtcggaaag
     2341 gctattgggc tcccggaact ggtggcgggg gcctggatcg gtgggaccat cgacacgacc
     2401 ggcgccgtgg tggcagcggg cgcgttgtat ggcgacgccg ccatgaaggt ggccaccatc
     2461 gtgaagttct cgcagaacgt cctgatcggc ttcgcagcct tcctgctggc gatcgcctgg
     2521 tcggtcaagc gtggggctga aaccggtgaa aagcccagcg ccatggtgat ctgggagcga
     2581 ttcccgaagt tcgtcctcgg cttcctggta gcctcgctcg tcttctcctt cttcctgagc
     2641 ccggatacca tcaacgcggt caagggcccg ctgaacttca tccggctgct gacgtttgcg
     2701 ctggcctttg tctgcatcgg cctggagacc aacttcaagg agttgctgca gttggagggc
     2761 ggccggccgg ctgcggcctt cctcggggcc caggcattca atctcatctg gacgctgatt
     2821 atagcgttcc tgctgttcgg cgggatcctg ttctcgatgc cggtcatcaa gtagcgagct
     2881 ctttcagccc ccacgtcggc ccatcccttg ctttggcaat ggcctaggga tgggtcggcg
     2941 agggggcgtt tccttgttct ctcgttgtcg taggtgggtc cgacgatggc ttctgatcta
     3001 agcctcagac ccggtcgctt ggagccatct cttccccgag accaggagcg atctctggga
     3061 aaagtccggg aagacgcgga acgggaccat atcctctccg tcctcaagct gacagagggg
     3121 aggcgggggc gcgcggccca gatcctgggg atcagtcgga agactctctg gaagaagttg
     3181 aagcacctgg gggtctcccg atctctggat gttacctaaa ggtaacactc caagatgact
     3241 tgttaccttt cggtaacatt ttcatctggg tggtttccct cgaatgtctt gctctcaagt
     3301 gattcgaagg ggaatctgac tcggcagaaa ccttgctctt gtcctaacgc ggtgactcag
     3361 cagagagcct aggaggatcg gagagtgccc atcgtcgtga agaaggtccg gaggttgggg
     3421 gcaggaggag gcggggcggg cggaggcggg agcacgtcgt cgctccggcc cgagttcgtt
     3481 ccaaagacac ctgcctgctc caaggcttgc cccaatcatc acgacattcg ggccgtcctg
     3541 accaccatcg cccaggccga tgcgcacggc aagaccagcg accaggccct cacggaggct
     3601 ttttgcctcc tgaccgatct gaaccctctg cccgcctcct gcggccgggc ctgcccccat
     3661 cagtgcgaac tccagtgcta tcgtctcgac gtggacgagg cggtctcgat caattgtctc
     3721 gaacggtaca tcggcgacta cgccctagaa aaggggctgc cccttactcc cctcacagca
     3781 gaccggcatc ccgaacgggt tgcggtcatc ggatcgggac ctgcgggcct ttcttgcgcg
     3841 taccagctgg cacgccgagg ctattcagtc acggtcttcg aggccttccc caggccgggc
     3901 ggcatgttgc ggtacgggct tccgagctat cggctgcccc gccaggtgct tgacggcgag
     3961 atcactcgtc tcggatccct ggggatcgaa atccgctgca acaccgccct gggaaaagag
     4021 gtcacactcg acgcacttcg ccaggaatat tcggcgatct tcgtcggtat ccgggcgcag
     4081 cttgggacga agctggggtt gcccggcgag gacgccccga atgtcctgtt ggcggccgaa
     4141 ttccttcacc gggtgaacgg cggcgagcgg ttggtcgtcg gagggcgggt cctggttgtg
     4201 gggggaagcg acacggctgt ggatgcagcg cgcgtggcgc ggcgactggg ggcccaggcg
     4261 accatcgtct cgcgacggcc cacccaggag atgcccgcca tccgccacga agttgaggag
     4321 gcgcagcgcg agggggtgca gatcgagggg ccggccgtcc ccttggtgat cgtgacggaa
     4381 gccggacggg ccaccggcct cacctgtcgc cgggagactc cgtccggaca ccctgggtcg
     4441 gctgaatccg aattcttcct gccggcggac agcatcatcg tcgcggcgta gcccgagcgg
     4501 gattacacca acctggaatc cctctgcggg gaggacggct ccatcctgac cagcgatcag
     4561 gcagagacga tcgttcccgg cacctttgcg gccagcgacg acctggatct gggcatcgtt
     4621 agcaccgccc tgtatcgcgg gcgccgggtc gccgagacca tccatgcgag gttccgggga
     4681 attcaagagt cggcgccgat cctgcctccg gtcatcggca aggacaggat gcggctcggt
     4741 tacttcacca agcagccgcg gacgccgctg gcaacgatcc ctctggagga gcggctccag
     4801 catccggaac aggaggtcaa tcttggattg accgagaccc agatcatcgc cgaggcgagg
     4861 cgctgcctga gctgcggtct gtgcttcgag tgcgacacct gctggcagta ctgccaggag
     4921 caggctatcc agaagccggc ggagaaagga tctccatacc ggatcaagct ggagttctgc
     4981 accggctgca aaaagtgcgc agaacagtgt ccctgcggct acatcgagat gcggtaggac
     5041 tcatcacgcg ggcaaagccg cccaggagga accccgatca tggctgccga cgagaagaag
     5101 ctgccgccca cacccaagct ggatgaactg gagagcggcc cctggcccag tttcgtcaag
     5161 gagatcaagc gggccgccga aaagaacccc atgagccagg atctgctcgg cgtcctggag
     5221 ctttcctaca cccagaagat cgcccactgg aaacacgggg ggatcgtggg cgtccggggt
     5281 tacgggggcg gggtcatcgg tcgctactgc gacatcccgg aacagtttcc caacgtcgcc
     5341 catttccaca ccctccgggt gaaccacccg gcaggctggt tctacaccac gaaggcgctg
     5401 cgcaatatct gcgacctgtg ggagcggcac ggctccggac tcactaacct ccacggatcc
     5461 accggggaca tcatcctgct ggggacacgc accgaagagt tggaggccac gttcggcgag
     5521 ttgacccagg cgggtttcga cctggggggc tccggctcgg tcatccggac gccaagctgt
     5581 tgcgtgggtc ccgcgcgttg cgagtgggcc tgctttgaca cgctccgatt catctatgac
     5641 gtgaccatgc acttccagga ctcgattcac cggccttcct tcccctacaa gttcaagatc
     5701 aaggcctccg gctgcgccaa cgactgcgtg gcggccatcg cccgcgcgga tctcagcatc
     5761 atcggcacct ggcgcggccc gatccaggtg gaccaggatg ccgtgggggc atacgccaag
     5821 gatggcatcg acatccagaa agacgtctgc gacccgtgtc ccagcaagtg catctcttgg
     5881 aatggcacga cgctcgctat cgacaatccg tggtgcgtca agtgcatgca ctgcatcaac
     5941 gtgatgccca aggccctccg gcctggccgc gagcggggcg cgaccattct gatcggcggc
     6001 aaggccccca tcctgaaagg ggcgatgctt tcctcggtgc tggtgccctt catgcagatg
     6061 gatccgccct acaccgagtt cacggccctg gcagagaaga tcctggatgt ttggggcgag
     6121 gaagggaaga accgggagcg ggtgggagag ttcatccagc gcgtggggct gggtaatttc
     6181 ctggaggcca tcggcctcga gcccatgccc gagatggtgg ctcaccctcg cgagaacccg
     6241 tacatcttct acgaagagta ccttgaagaa gagtcgaccc agagccagtc gtaatcggcg
     6301 cggccgaagg gagccgtgcc ttcagaaggg agaatcgaga catggccgag accgctgtcc
     6361 agcgcaagac cgacatcggg cccccccact accagatgtt cctgcctccc atggtcaaga
     6421 agaactacgg ccagtggcgg taccacgaca tcctcaagcc cggggtcttg gtccacgtgg
     6481 ccgagtcagg cgacaagctg tacaccgtgc gcgccgggac cccacggctg ctgagcatcc
     6541 agacgatccg cgagatctgc gacctggcgg atacctactg tgacggatac ctgcgcttca
     6601 ccagccggca caacattgag ttcctgctca ccgattcggc caagatcgag cccctcatca
     6661 cggatctggc cgcccgcggc tttcccgtgg gaggggcggg caacgccatc tccagcatgg
     6721 tgcacaccca gggatggata cactgccaca ccgccgccac ggacgcctcc ggcatcgtca
     6781 aggccgtgat ggatgagctg atcgaccact tcaccacgac gaagctcccg gccaagcttc
     6841 gcctggccct ggcctgctgc ctgaacatgt gcggcgccgt gcactgctcc gacatcgcca
     6901 tcctggggat ccatcgccgg ccacccaagg ttgaccatca gcggctctcc aaggtctgcg
     6961 agattcccaa cgttgtcgcc tcctgtccca ccgccgccat tcgacccacg cggatcgacg
     7021 ggaaggagac ggtggaaatc atcgaagaga actgtatgtt ctgcgccaac tgctacacgg
     7081 tctgcccggc gctgcccatc aacaacgcgc ggaacgacgg ctgctccatc tgggtcgggg
     7141 gcaaggtcag caatgcgcgg cacgccccca tgttctccaa gctggccgtt cccttcctgc
     7201 ccaacaaccc tcctcgctgg cccgaggtgg tggcggcggt gaaaaacatc gtcgaggtct
     7261 gggcggcgga tgcccggaag tacgagcgga tgggcgaatg gatcgagcgc atcggctggc
     7321 cgcgcttttt ccagaagacc ggtatcccgt tcacgaagca gcacattgac gattaccgac
     7381 acgcggacca gacgtggcaa cgctcgatcc acttcaaggc gtgaggaggc ttccatggcc
     7441 gaaccggtga gcaccgagga gttggccgag gccatgtatc acatggtgca ggagagcgca
     7501 gggagccgga ggctccggcc gacggatctg acaagggcga tgatcgagcg cttcggcccg
     7561 gagcgctgca ccaaggagca ctgcaagcag gcgctgcggc ggctcgtgga tgccgagcgt
     7621 tgcgtctaca ccttctacga tggaagctac gtcgagcttc ccccgcaaga gaagggcaag
     7681 gcagaggcat gaatgatttc catgaggtcc acatgctgga tccggtaagc gtccataccg
     7741 ccgcctgccc gcgggtgatg ctctgcggcc tcaggggaag caccggcaag acgctggtcg
     7801 ccctggggct gatcagcgcc tgggaggcgc ggggtgtctc cgtcgcgccg tgcaagaagg
     7861 gcccggacta cattgatcct gcctggcaca cccgcgcggg aacgcgcccg tgccgcaacc
     7921 tcgacacctt cctgatgagc gacgagcaga tccggcgctc ggtgggagag catggccgca
     7981 cggccgatct cgccctcatc gaggccaacc acggcatctt cgatggcgtg ggggccgagg
     8041 cggcggccag ttcggccgag ctggccaagc tgctccaggc gccggtggtg ctggttgtcg
     8101 attgcaccaa gaccacccgg accgccgcgg ccatggtgct cgggtgccag cacatggatc
     8161 cggacttgcg cctagccggg gtcatcctaa accgcgtggc cacggaacgc caggaacgtg
     8221 ctgtacgaga ggcagtagag acgacctgcg ggctgcccgt cctggggagt ctcccgcgac
     8281 tgtcgaacct ggaatttccc gagcgacatc tggggctgct taccccggcc gagcatcccg
     8341 ccgtggcgga gcccatccgg atcgcccgcc aggcggtgga ggcgcatgtg gacctggacg
     8401 cgatccagcg cctggcggcg actgcgcccc ctctggcgtg gaccgcggaa gcggagtctc
     8461 ggatcgatgg ccgaggcgtc cggatcggag tcgtccggga cagcgccttc accttctact
     8521 accccgagaa tctggaggga ctcgagcgac agggtgccga gatcgtggaa gtcaacgccc
     8581 tgaaggatcc cgacctgccc ccgctggacg cgctctacat cggcgggggc taccccgaga
     8641 cccacgccgc cgccttagcc gccaatgtcg ggttccgggg cgcgctccac cgagaaatcg
     8701 aggctggact tcccatcatc gccgagtgcg gcgggctgat ctatctcggc gaggccatgg
     8761 tcacggacgg gttcgcccat cccatggtgg ggatcttccc cgtgaccttc gagctgggca
     8821 agcggccgtg ggggcacggc tacaccgtgg tcgaggtgga cacccccaac ccattcttcg
     8881 cgccggggac ggccctccgg gggcacgagt tccggtactc ctacgtgcgc tccgcccgga
     8941 ccgaggacct gcgcctggcc tttcgcatgc agcgcggcac cggcttcgac acgaagcgag
     9001 acggcctcgt gcacaagaat gtcctggcct ccttctgtca tttccatgcg gccgggacgc
     9061 ccgagtgggc tccggcactc gttcgtcagg cccacctgca cgcccagcgc cgacgcctgg
     9121 cggcgagatc gagggatgtg ctccacaggg attggacgac accggaacct gcaggctcgg
     9181 ttgcccttcc cgtcgggacg atatgaccat cctttgacct gggccactcg gcggtggggc
     9241 agtggcagga ctgaggaact tacctgagga gagaagagaa tatgagtacc
//
LOCUS       DBNGZC010001122         1612 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1565284, whole genome shotgun sequence.
ACCESSION   DBNGZC010001122 DBNGZC010000000
VERSION     DBNGZC010001122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1565284"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..1310
                     /locus_tag="AC16G0_19400"
     CDS             255..1310
                     /locus_tag="AC16G0_19400"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HIR5101828.1"
                     /translation="MEDTGRRFDPSKLSAFCVAVLEKAGVSRENAEIVAHSLLAANVR
                     GVDTHGITRLPIYVERLQAGLTKGRVQGVVIFETPTTAVYDGQDGLGQVVGTKAMRLA
                     IAKAESAGVGLVTARNSTHYGTAGYYAMMALDKDMIGIALTNSPSLMAPWGGKRAFLG
                     SNPIAIAVPAGKEKPFVLDMATTVVARGAIILAAKKGETIPISWGLNKDGEPTTNAKE
                     ANEGALLPLGGHKGYGLAMAIEVLVATLAGGPFGPHIGELYKNPTRTQGVGHFFGAIR
                     IDRFRPAAEFKADMDAMIQEVKAEPLAKGFDKIMVAGEPEFLVEQERREKGVPLSDAV
                     VNDLSKLGASIGIPFDA"
BASE COUNT          305 a          499 c          521 g          287 t
ORIGIN      
        1 tgcccacccg gagactgttc ggaacgatcc agggatcgtc aggtggactc aacctccgcc
       61 ccgagatgcc tcggggattg cgccgatcaa ggatccaagc ccagtttctc cttgacagtg
      121 cttgaggcca tacggcatgc ttcggcgact tcggctggcc aacgtcgggt cactggtctg
      181 cccgtactca gcgaaggctc tcactggatt catgcacccg gaagaacacc caggatttgt
      241 ggagggacga tagcatggaa gataccggac gccgcttcga cccgtcgaag ctctcggcat
      301 tttgcgtggc cgtcttggag aaggcgggcg tatccagaga gaatgcggag atcgtcgccc
      361 actccctgct ggccgccaat gtgcgagggg tggacaccca tggcattaca cgcctcccca
      421 tatatgtgga gcggctgcag gcagggctca ccaaaggccg agtccagggg gtcgtgatct
      481 tcgagacccc aaccaccgcc gtctacgatg ggcaggacgg gctggggcag gtcgtgggca
      541 ccaaggcgat gcgactggcc atcgccaagg ccgagtcggc aggggtaggg ctggtgaccg
      601 cgcgcaactc cacccactac ggtacggcgg gctactatgc catgatggcg ctggacaagg
      661 acatgatcgg gatagccctc accaactccc cctccctcat ggcaccctgg ggtgggaagc
      721 gggcctttct gggatccaat ccgattgcca tcgctgtgcc tgccgggaag gagaaaccct
      781 tcgtgctgga catggccacc accgtcgtgg cacgaggcgc catcatcctg gccgccaaga
      841 agggggagac catccccatc agctggggct tgaacaagga tggcgagccg acgaccaacg
      901 ccaaggaggc caatgaaggg gcgctccttc ccctcggcgg tcacaagggt tatggtctcg
      961 ccatggccat agaagtcctg gtggcgacgc tggccggcgg ccccttcggt ccccacatcg
     1021 gagagctgta caagaacccc acgcgcaccc agggcgtggg ccacttcttt ggcgccatca
     1081 ggatcgatcg gttccgcccg gctgccgagt tcaaggccga catggacgcc atgattcagg
     1141 aggtgaaggc ggagcccctg gccaagggct tcgacaagat catggtcgcc ggcgaacccg
     1201 agttcctcgt cgagcaggag cggcgggaga agggcgtccc cctcagcgat gccgtggtga
     1261 atgacctatc caagctggga gctagcatcg gtatcccctt cgacgcctaa caggctgcgt
     1321 gtccggatgt ctccgggtgt agagtcgttt gggtgtggtc cggttccccc tcatccgatg
     1381 ggctgcatgc tcgcccgagg tggacaatct ggtaaatgaa aaagcgccga gagcccatgg
     1441 ctctcggcgc tttgcgtttg cgcgacagaa cgtgccggct acttgccggt gaacgcgccg
     1501 gtccagagca ccccttcctt ggccgagggc ttggatgaaa tctggccggt gtccgcgagg
     1561 aagtcgaggt gcttctgcac catctgctcg ttcatcatgc cgtccgggga ca
//
LOCUS       DBNGZC010001123         1852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1565348, whole genome shotgun sequence.
ACCESSION   DBNGZC010001123 DBNGZC010000000
VERSION     DBNGZC010001123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1565348"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..396)
                     /locus_tag="AC16G0_19405"
     CDS             complement(109..396)
                     /locus_tag="AC16G0_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIR5101829.1"
                     /translation="MAAYIIVDVDVQDVTRFETYKQLVPATIAQYGGRYLVRGGKHET
                     LEGNWRPTRLVVLEFPSGEAARRWYESEEYQKIKPLRREHARGDMVLVEGV"
     gene            complement(555..>1852)
                     /locus_tag="AC16G0_19410"
     CDS             complement(555..>1852)
                     /locus_tag="AC16G0_19410"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004035 - alkaline phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0106219 - zinc ion sensor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HIR5101830.1"
                     /translation="AAPARAKNIILLIGDGMGSSQFGVAWLYSNRVLNNDLRMIEVMR
                     AGRTAYLANDTADATVTESAAAATQIACGVKVPARAPGMGADGKTPCKTILEIAKERG
                     MATGLVTTSGITDATPASFSAHVSDRSDEASVAAQELAVGVDILMGGRKNFFLPQAAE
                     GSRKDGRNLLEEAQKAGYTVVGTAEELQKAPAGKILGLFNMGNMAFEIDRASTKEPSL
                     AAMTTKTLDVLSRNPKGFFAMIEGGRIDHASHRNDAAGTIQDMLAFDQAVGVALDFAK
                     KHPDTFLLVTADHETGVMSLIGPSKTGKEYAGIDLEAIRKVKASHELIAAKLGKSPTP
                     QRVKAVVKEYMDIEITDDEAKMVASDTIRGLDPTDHNYAYPPRLGFVLRPYLPVDWGS
                     QTHTASPVMAFGLGPGSEKIVGFRHNTELFSIMKTALGN"
BASE COUNT          295 a          634 c          537 g          386 t
ORIGIN      
        1 aggtaggcga gcacattcgt atccacccca accgacgtcg tcactgctgc cccttcagca
       61 tcccccgtct tggcgattcg gggatcccgg tggccatggc ctgcccggct agacaccctc
      121 cacgaggacc atatcgcctc gggcgtgctc gcgccggagc ggcttgatct tctgatactc
      181 ctcggactcg taccaccgtc tggcagcctc cccactcggg aactccagca cgacgaggcg
      241 ggtagggcgc cagttcccct ccaacgtctc gtgcttgccg ccccgcacca ggtagcgtcc
      301 cccgtactgg gcgatggtgg cagggactaa ttgcttgtac gtctcgaatc gagtcacatc
      361 ctgcacgtcc acgtccacaa taatgtaggc cgccatgttt tcctccaacg aatcaggctg
      421 cattccaatt caagaccggc aatttctcaa gggcgatcct gggggttccg ggttttcggt
      481 ttccttgctc tagatggctc ctctcatact tgaaggcgtg gtggcttggt ggtaaataga
      541 ctttcgcgtc ggggctagtt ccccagcgcc gtcttcatga tggagaacaa ctcggtgttg
      601 tggcgaaatc caacgatctt ctcggacccg ggccccaggc cgaaggccat gacgggcgat
      661 gcggtatgcg tctggctccc ccagtcgacc ggcaggtagg gacgcaggac gaagcccagc
      721 ctgggggggt acgcgtaatt gtggtcggtc gggtccagcc cccgaatggt gtcggacgcc
      781 accatcttcg cctcgtcgtc ggtaatctcg atgtccatgt attccttgac caccgccttg
      841 accctctggg gagtcgggct cttgcccagc ttggccgcga tcaactcgtg agacgccttc
      901 accttccgga tggcctccag atcgatgccg gcatactcct tgcccgtctt gctggggccg
      961 atcagcgaca tgacgccggt ctcgtggtcc gcggtgacga gcagaaacgt atcgggatgc
     1021 ttcttggcga aatccagcgc cacgcccaca gcctggtcga aggcgagcat atcctggatc
     1081 gtccccgccg cgtcgttgcg gtgcgaggcg tggtcgatcc gccctccctc tatcatggca
     1141 aagaaaccct tggggttcct ggagagcacg tccagagtct tggttgtcat cgcggccagg
     1201 ctgggttcct tggtgctggc ccggtcgatc tcgaaagcca tgttccccat gttaaagagt
     1261 ccgaggatct tcccagccgg cgccttctgt agttcctcgg ccgtccccac caccgtgtac
     1321 cccgccttct gggcctcttc cagaagattc cgcccgtcct tccggcttcc ctcggccgcc
     1381 tggggaagga agaaattctt ccgaccaccc atgaggatgt ccacgcccac ggcgagttcc
     1441 tgcgccgcca ccgaggcctc gtcgctccgg tccgagacgt gggccgagaa ggatgccggc
     1501 gtggcatcgg tgatccccga ggtcgtcacg agccccgtgg ccatgccccg ttccttggcg
     1561 atctccagga tcgtcttgca cggggttttc ccatcagcgc ccatgccggg cgcacgagcc
     1621 ggcaccttga ccccgcaggc gatctgcgtg gcggccgcgg ccgactccgt caccgtcgca
     1681 tcggccgtgt cgttcgcgag gtacgccgtc cgaccggccc gcatgacctc gatcatcctc
     1741 aaatcgttgt tcagcacgcg attgctgtac agccaggcta ccccgaactg gctcgacccc
     1801 atcccgtcgc cgatcaagag aatgatgttt ttcgccctcg ccggcgctgc cc
//
LOCUS       DBNGZC010001124         3218 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_2054174, whole genome shotgun sequence.
ACCESSION   DBNGZC010001124 DBNGZC010000000
VERSION     DBNGZC010001124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_2054174"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="AC16G0_19415"
     CDS             complement(<1..1210)
                     /locus_tag="AC16G0_19415"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5101831.1"
                     /translation="MQPDLGGLRGRLEAIVGGAYVGAGPVEGTRYAVDGMFPALSVQP
                     GSQDEVATILAACAEAGAAIIPWGGGTAMGLGNPPTRADVAVDLGRLNRVVDHDVANL
                     NVSVEGGMRLADLQATLALQRQYLPLDPPAEMKATIGGLMAANGSGPARLLYGTARDW
                     VLGMRIVLPDGERIRCGGKVIKNVSGYDMNKLFIGSLGTLGIITEVTFKLLPMPGVRA
                     SVIGLFPELGQAAGIVAKVLDSVLLPEAIEFLNPEAVRLLAPALGLEGPTGNGLAVAL
                     AGTPETVERQVRDFVAIFQEGQADRTTTLQGDRTVPAWQAIRDVFDLPSGPPTERVIC
                     KIAVPISRTAETLAAAEELGRHHNLRAAVTGHAGSGIVVACYLLPKDAPPAEALADSL
                     EEFRREVEARE"
     gene            complement(1200..2600)
                     /locus_tag="AC16G0_19420"
     CDS             complement(1200..2600)
                     /locus_tag="AC16G0_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR5101832.1"
                     /translation="MAVRALVSDLQQIVGKPNVLSSGVDLQLYGYDAYLEERRPDAVV
                     FVNSTEEVAGVVRACNKHAVPFVPRGGGTNLSGGTIAIKGGVVVEMIRMNRILEVDRA
                     NLRARVQPGLFNLEFGNALAPLGYQYLPDPASQKAATLGGNVAENSGGPHCFKYGVTT
                     NHVLGLTLVLSDGSVVRTGGKAPDVPGYDLTGAFVGSEGTFGICTESVTKIVHLPEGV
                     KTLLAIYDTIEEGSESVSAIVAAGMVPATLEMMDQLVIKAVEESIHCGFPLDCATVLI
                     IEVDGLKDDLEIQAKAIADLCRAHGAREVRVAKDDAERAALWAGRRGAFGAVARLTPS
                     YLVCDGTVPRTALPKVLRKVAEVGKKYNLQIPNVFHAGDGNLHPLILFDWRDAEQKAK
                     VLRAGMEILQLCVELGGTISGEHGVGLEKIDAMRLVQGEPESRAQLRLKKAFDPPNLA
                     NPGKMFPLPEEVRHAA"
     gene            complement(2951..>3218)
                     /locus_tag="AC16G0_19425"
     CDS             complement(2951..>3218)
                     /locus_tag="AC16G0_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003282764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HIR5101833.1"
                     /translation="LAAFETGVLYQLVHALALIGVAWATTRWPGRATVIAGWLFLAGT
                     LLFSGSLYALALSGAFFFGAITPLGGVALIAGWLVLAWAAWQER"
BASE COUNT          541 a         1152 c          998 g          527 t
ORIGIN      
        1 cctctctggc ctccacctcg cgtcgaaatt cctcaaggct gtcggccagg gcttccgccg
       61 gaggcgcatc tttcgggagc aggtaacacg ccacgacgat gccgctgccg gcatgccccg
      121 tcaccgcagc ccggaggttg tggtggcggc ccagctcttc tgccgctgcc aaggtctctg
      181 ccgtccggct gatgggcacg gcgatcttgc agatcacccg ctccgtcggc ggaccggacg
      241 ggaggtcgaa tacatcgcgg atggcctgcc acgcggggac cgtccggtcg ccttgaaggg
      301 tcgtcgtcct gtccgcctgt ccctcctgga agatcgcgac gaaatcccgc acctggcgct
      361 ccaccgtctc cggagttccc gccaaggcca ctgccaagcc gttgccggtc gggccttcca
      421 ggccgagggc cggcgcgagc agtctgacgg cctccgggtt caggaactca attgcctcgg
      481 ggagcagaac agaatccagg accttggcca cgatcccggc tgcctgaccg agttcaggga
      541 acagcccgat cacgctggcc cgaaccccgg gcatggggag cagcttgaac gtgacctcgg
      601 tgatgatccc cagggtgccc aggctgccta tgaacagctt gttcatgtcg tagcccgaga
      661 cgttcttgat gaccttgccg ccgcaacgga tccgctcgcc gtcgggcagg acgatccgca
      721 ttcccaggac ccagtcccgc gccgtcccgt acaggagccg ggccgggccg ctgccgttcg
      781 ccgccatgag cccaccgatc gtggccttca tttctgccgg tgggtccaag gggagatact
      841 gtcgctgcaa cgccagcgtc gcctgtagat cagccagccg catgccgcct tccacgctga
      901 cgttcagatt ggccacgtcg tgatccacca cccggttgag tcgtcccagg tccacggcca
      961 catccgcccg ggtgggggga ttgcccaacc ccatggccgt cccgccgccc caggggatga
     1021 tggccgcccc cgcctcggcg caggccgcca ggatcgtcgc cacctcgtcc tgagagcccg
     1081 gctgcacgct gagcgccggg aacatcccat ccaccgcata gcgggtccct tcaaccggac
     1141 cggcacccac gtaagccccg ccgacaatcg cctccagccg tccccgcaat cctcccagat
     1201 caggctgcat ggcgcacctc ctcgggaagc gggaacatct ttcctggatt cgcgaggttt
     1261 ggaggatcga aggccttctt cagccggagc tgcgcccggc tctccggctc cccctggacg
     1321 agccgcatgg catcgatctt ctccagcccc accccgtgct cgccgctgat ggttccgccc
     1381 agctccacgc agagctgcag tatctccatg ccggccctaa ggactttggc cttctgttcg
     1441 gcatcccgcc aatcgaacag gatcagcggg tgcaggttgc cgtccccggc gtggaacacg
     1501 ttggggatct gcaggttgta tttcttgccc acctcggcca ccttgcgcag gaccttgggg
     1561 agcgcggtgc gaggaaccgt gccatcgcag acgaggtagc tgggtgtgag gcgcgccacc
     1621 gcaccgaagg cgccacgcct gccagcccac agggccgccc gctctgcgtc gtccttggct
     1681 acccggacct cgcgggcacc gtgcgcccga cacaggtcgg cgatggcctt ggcctgaatc
     1741 tccaggtcgt ccttcagccc gtccacctcg atgatgagca ccgtggcgca gtccaggggg
     1801 aacccacagt ggatggattc ttccaccgcc ttgatgacca gctgatccat catctccagg
     1861 gtggccggca ccatcccggc ggccacgatg gccgacacgc tctcgctgcc ctcctcaatc
     1921 gtgtcgtaga tggccagaag ggtcttcacg ccctcgggca ggtgcacgat ttttgtcacg
     1981 ctctcggtgc agatcccgaa ggtgccctca ctccccacga aggccccggt gagatcgtag
     2041 cccgggacgt cgggtgcctt tccgcccgtc cgcaccaccg agccgtcgga caggaccagc
     2101 gtcaaaccca ggacgtggtt cgtggtcacg ccgtacttga agcagtgggg cccgcccgag
     2161 ttctcggcca cattcccccc cagggtagcc gccttctggc tggccgggtc cggcaagtat
     2221 tggtagccga ggggggccaa ggcgttgcca aactccaaat tgaaaaggcc cggctggacc
     2281 cgggcccgaa gattggcgcg gtcgacctcc agaatccggt tcatccggat catctcgacg
     2341 acgacgccgc ccttgatcgc gatggtgccg ccgctgaggt tcgtcccccc gccccgaggg
     2401 acaaagggaa cggcgtgctt gttgcaggca cgcacgaccc cggccacctc ttcggtggag
     2461 ttgacgaaga ccaccgcgtc cgggcgccgc tcctccaggt atgcgtcgta cccgtaaagc
     2521 tggaggtcga cgcccgagct caggacattc ggtttgccca cgatttgctg caggtcgctg
     2581 acgagcgcgc gtacggccat tggcctcctc ctgatggggc cgcgccgctc cgcaaagggc
     2641 cggagtctcg cgggccagct acatgagaca atggcaacag gctgccgacc tcggccacgt
     2701 gcgtctgctg gtaggttggg ggtctcggat ttcccgccgg tcccaggcca tgaccctacg
     2761 ggagggactg acaagagtac gtgccacccg gtaaatctgg gtcttgagga ccgactcaca
     2821 catccactcg aagcagagcc ggggcgacgc gggaaaatcc cgacgccgct ctcacggtat
     2881 ggccagcccg aggtggcgtc aagcacggat tttcagcttg cctgcacaac cggacaggtc
     2941 ctgccgcggg tcagcgctcc tgccacgcag cccaggcaag gaccagccag cccgcgatca
     3001 gggccacccc gccgagaggc gtgatcgcgc cgaagaagaa agccccgctc agggcgagcg
     3061 cgtacaagct gccagaaaac aggagcgtgc ccgcaaggaa cagccagccc gcgatgaccg
     3121 tagcccttcc cggccagcgg gtcgtagccc aggctacgcc gatcaacgcc agggcgtgca
     3181 ccagctggta gagcacgccc gtttcaaagg cagccagc
//
LOCUS       DBNGZC010001125         1891 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Candidatus Methylomirabilales bacterium isolate
            MAG05494 SRR21617313_k127_1173570, whole genome shotgun sequence.
ACCESSION   DBNGZC010001125 DBNGZC010000000
VERSION     DBNGZC010001125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414902
            Sequence Read Archive: SRR21617313
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Methylomirabilales bacterium (soil metagenome)
  ORGANISM  Candidatus Methylomirabilales bacterium
            Bacteria; Candidatus Methylomirabilota; Candidatus
            Methylomirabilia; Candidatus Methylomirabilales.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 00:51:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,886
            CDSs (total)                      :: 3,860
            Genes (coding)                    :: 3,804
            CDSs (with protein)               :: 3,804
            Genes (RNA)                       :: 26
            tRNAs                             :: 24
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 56
            CDSs (without protein)            :: 56
            Pseudo Genes (ambiguous residues) :: 0 of 56
            Pseudo Genes (frameshifted)       :: 4 of 56
            Pseudo Genes (incomplete)         :: 36 of 56
            Pseudo Genes (internal stop)      :: 19 of 56
            Pseudo Genes (multiple problems)  :: 3 of 56
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Candidatus Methylomirabilales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR21617313_k127_1173570"
                     /isolate="MAG05494"
                     /isolation_source="soil"
                     /db_xref="taxon:3159508"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="24.55 N 113.64 E"
                     /collection_date="2019-12-01"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..>1891
                     /locus_tag="AC16G0_19430"
     CDS             106..>1891
                     /locus_tag="AC16G0_19430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5101834.1"
                     /translation="MRSISIRNVRSLLALLLILLLAMVWPTGASAQSSTPPTPQFAFL
                     GFIQEATLDTAGAICTPTEPRLAGGTMTVNGIKMIVPCNSIIQLPANTMTWADLFDPA
                     KSTAVGTFLGTVPANPLVGGLKTGLALADNPMPFPSFEVSVNGNVVGKNPDGTDKYIV
                     GLIVPVTQQGLNGSSGLVSFIDYVSGSFRVGGVVGDANCSQGGVPGGGLLCSGTLVQF
                     NDPVTNAGPDGTSGRWGLTHSPDPRFSGDTANVTIHTSTGFPLCIPRVAPPGIDTECP
                     LKNRPLNSDARFGAPDPFLAGTAPRKIFDMPTPAAADLDPTLPDPRKQIPLMVGDQVV
                     YAGTLYKIAPLLLRTDPITGATIPDNTATNSYISAHTVEDVLGIFTQPGVPPAYVYIE
                     AFLIGTNGAAVNGILQEASTRMTVVGFTSDPTRLVDIYAQDVNPCSGQVSLRLLASED
                     PAVDAIRGRFVHRVLGGLFMPPTRNYTIRSRTQTLDPISGLRVDFVAANGILTGEFSL
                     PNFEYVFPENHRLGDPFLPNNYQDMPFLALGNGPVDGFGTASPIVGQLNPWPGSPTPA
                     SVACPTGGGTTPVVRIDPPVVSVGPGAAV"
BASE COUNT          326 a          670 c          565 g          330 t
ORIGIN      
        1 caggctgagt cctgagaagg cacgcatcta cccagcgtgt ggtttatcgt ctgtgcgcca
       61 catgcactct acaactgtta cttgtcacac acgggaggaa tcaccatgag atccatatct
      121 attcggaacg tccgatcgct cttggctctg cttctgattc tgcttctggc gatggtctgg
      181 ccgaccggag cgtcggcgca gtcgtcgacg ccaccgacgc cgcagttcgc cttcctgggg
      241 ttcatccagg aagccactct ggacacggca ggggccattt gtacaccaac cgagccgcgg
      301 cttgcgggcg gcacaatgac cgtcaacggc atcaagatga tcgtgccctg caacagcatc
      361 atccagttgc cggcgaacac gatgacctgg gccgacctgt tcgaccccgc gaaatcgaca
      421 gcggtcggca cgtttcttgg cacggttccc gcgaacccgc ttgtcggggg tctgaagacg
      481 ggcctcgcac tggcggacaa cccgatgccc ttcccctcgt tcgaggtgag tgtcaacggc
      541 aacgtggtgg ggaagaaccc ggacggcacc gataagtaca tcgtcgggtt gatcgtcccc
      601 gtcacgcagc aaggcctgaa cggcagctcg ggactcgtca gcttcatcga ctacgtgtcc
      661 ggctcgttcc gcgtcggtgg agtcgtcggc gatgcgaact gctcgcaggg aggcgtgcct
      721 ggggggggac tgctctgcag cgggaccctg gtccagttca acgacccggt cacgaacgcc
      781 gggccggacg gcaccagcgg gcggtggggc ttgacgcaca gccccgatcc gcgcttcagc
      841 ggcgacactg ccaacgtgac catccatacc tccacgggct tcccgctgtg catcccgcga
      901 gtggcgcctc cgggcatcga tacggaatgc ccgctgaaga accggcccct gaactccgac
      961 gcgagattcg gcgcccccga tcctttcctg gcaggcacgg caccccggaa gatcttcgac
     1021 atgccgacgc cggctgcggc ggatctggat cctacgctcc ccgacccacg taagcagatc
     1081 cccctgatgg tcggtgacca ggtcgtgtac gcgggcacgc tctacaagat cgctcccctg
     1141 ctacttcgca cggacccgat taccggagcc accatccccg acaacaccgc gacgaacagc
     1201 tacatctccg cccacacggt ggaagacgtc ctggggatct tcacccagcc gggcgtcccg
     1261 cccgcctatg tgtatatcga ggcgttcttg atcggcacca atggcgccgc cgtcaacggc
     1321 atcctccagg aggcctcgac gcgcatgacc gtggtcgggt tcaccagcga cccgacccgg
     1381 ctggtggaca tctacgccca agacgtcaac ccctgctcag gccaggtgtc gctgcggctg
     1441 ctcgccagcg aggacccggc cgtcgacgcg atccggggcc gcttcgtgca ccgcgtcctc
     1501 ggcgggctct tcatgccgcc gacccgcaac tacaccatcc ggagccggac ccagaccttg
     1561 gatcccatca gcgggctgcg ggtggacttt gtcgcggcca acggtatcct tacaggggag
     1621 ttcagcctgc ccaacttcga gtacgtcttc cccgagaacc atcgactcgg cgacccgttc
     1681 ctcccgaaca actaccagga catgccgttc ctcgccttgg gcaacggacc ggttgatggc
     1741 tttggcaccg cttccccgat cgtgggtcag ctcaatccgt ggcctgggag ccccacgcca
     1801 gcctcggtgg cctgcccgac cggcggcgga accacccccg tcgtccgcat cgacccgcca
     1861 gttgtctccg tgggcccggg cgccgcggtg a
//
